TSC1A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	TSC1A
Entrez Gene ID	337167
Full Name	TSC complex subunit 1a
Synonyms	fa99f04,wu:fa99f04,zgc:63657
General protein information	Preferred Names TSC complex subunit 1a Names hamartin tuberous sclerosis 1a
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005171797.4	XP_005171854.1	hamartin isoform X2
XM_005171797.3	XP_005171854.1	hamartin isoform X2
NM_200052.1	NP_956346.1	hamartin
XM_005171795.4	XP_005171852.1	hamartin isoform X1
XM_021476019.1	XP_021331694.1	hamartin isoform X3
XM_005171796.3	XP_005171853.1	hamartin isoform X1
XM_005171796.4	XP_005171853.1	hamartin isoform X1
XM_005171795.3	XP_005171852.1	hamartin isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TSC1	NP_000359.1
Bos taurus (cattle)	TSC1	XP_005213508.1
	TSC1	XP_001103846.2
Mus musculus (house mouse)	Tsc1	NP_075025.2
Xenopus tropicalis (tropical clawed frog)	tsc1	XP_002944681.2
Pan troglodytes (chimpanzee)	TSC1	XP_001168992.1
Gallus gallus (chicken)	TSC1	XP_415449.2
Canis lupus familiaris (dog)	TSC1	XP_005625233.1
Rattus norvegicus (Norway rat)	Tsc1	NP_068626.1
Danio rerio (zebrafish)	tsc1a	NP_956346.1

Gene Ontology
Bibliography

Related articles in PubMed

Heat-induced masculinization in domesticated zebrafish is family-specific and yields a set of different gonadal transcriptomes.
Ribas L, Liew WC, D?az N, Sreenivasan R, Orb?n L, Piferrer F
Proceedings of the National Academy of Sciences of the United States of America114(6)E941-E950(2017 Feb)

Transcriptomic profiling of mTOR and ryanodine receptor signaling molecules in developing zebrafish in the absence and presence of PCB 95.
Frank DF, Miller GW, Connon RE, Geist J, Lein PJ
PeerJ5e4106(2017)

Maternal exposure to the water soluble fraction of crude oil, lead and their mixture induces autism-like behavioral deficits in zebrafish (Danio rerio) larvae.
Wang Y, Zhong H, Wang C, Gao D, Zhou Y, Zuo Z
Ecotoxicology and environmental safety134P123-30(2016 Dec)

Genome wide analysis reveals Zic3 interaction with distal regulatory elements of stage specific developmental genes in zebrafish.
Winata CL, Kondrychyn I, Kumar V, Srinivasan KG, Orlov Y, Ravishankar A, Prabhakar S, Stanton LW, Korzh V, Mathavan S
PLoS genetics9(10)e1003852(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TSC1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSC1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa142569	XM_021476019.1 Latest version!	Danio rerio tuberous sclerosis 1a (tsc1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa00014	NM_200052.1 Latest version!	Danio rerio TSC complex subunit 1a (tsc1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa10423	XM_005171796.4 Latest version!	Danio rerio tuberous sclerosis 1a (tsc1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa10423	XM_005171795.4 Latest version!	Danio rerio tuberous sclerosis 1a (tsc1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa10424	XM_005171797.4 Latest version!	Danio rerio tuberous sclerosis 1a (tsc1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa10424	XM_005171797.3	Danio rerio tuberous sclerosis 1a (tsc1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa10423	XM_005171796.3	Danio rerio tuberous sclerosis 1a (tsc1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa10423	XM_005171795.3	Danio rerio tuberous sclerosis 1a (tsc1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TSC1A ( XM_021476019.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021331694.1 Danio rerio tuberous sclerosis 1a (tsc1a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa142569
Clone ID Related Accession (Same CDS sequence)	XM_021476019.1
Accession Version	XM_021476019.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	hamartin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGG AGCTGTGGAG CCCTTCCTCC ATATGTGGGA TGAGCACCCC TCCCAGTTCT 
AGAGGAATGT CCCCAACCAC AGTTTCTGAC ATCTCACTTA GTGCCTCACA CCTCTCAGGA 
CGGATCCCCA GCACCCCAGG GGATGGAAAG TGGACTCTAT CTCCCACCAC ACCCTCGGGG 
TCATCCCCTC TCCCATCGCT CTCAGAGGAA ATAGGGCAGG CTGCCCAAAA CACCAGCAGT 
ACCCAGGCTA AGACGTGCAA AGAGGTGAAG AAAGTCACAT CCAGAAGTAT TAATAACAGC 
AGTGAGAATG GAGGTGATGC CAAGACTCCA GTGTCGTTGG CACAGCTCTC TGACATTGTC 
AAAAGGACAG AGGGGGAGCG AGAGGATGAT ACAATCAATG AGGAGATTTT GAGTCTAACC 
CATGGGAAAC TAGACTTTGG ATCTAGGCCT GGTCTGGAAT CCTCATTTTC TGGCTCCTTA 
GACACCCTTC TCTCATCTCA TCCTAATCAT GAGCAGAATT TGGTATCCAC ACCAGACAGG 
GTTGAATGCT CGGCTAAAGC AGGAGAGCAG CAGCTTCCTA GGTCTCTCTG GCCGTTCACC 
CCCATTGAAA ATGGGAAGCA CAGTAAATGT TCTCTGTCTG ATCTCACAAA AGAATCCATG 
CCTTATGAGC CACTGTTTGA CCTCGCCCTG CCGAAGGTGG CATCTCTGTT TTTGGAGAGG 
AAGACTGCTG AGGCCTTTCG GAGGGTTGAA GGAGAAAGAG ATCACCAAGA TGAGATGGAA 
GGAGAGGATC TTTCTGTCAC CTCCCCTCTG GAGGTGTTGG ACCGGGTTAT CCAGCAGGGT 
CACGACACTC ATGGGAAAGT GCTGAAGAGA ACTTTCTCTT CTCCAAGCCA ATCATCAGAC 
CCACACTTGA AAGGTAAACA GCATAGCAAT AAAGGAAAAG GTCTTGTGCC TGCAGTGTCG 
GATGAGCTAG ACATGTTACG CAGTCAATTG CTGCTGCTGC ATAACCAGTT GCTTTATGAG 
AGACATAAGA GAGAGCAACA TGCGCTTCGT AACCGTCGTC TGTTTGGCCG CATCGTCAAC 
ACTACTGCAC TGGAAGAGCA AAATAACTCC ATGAAAACGC AGCTCAAACT TCAGGAAGTT 
GAGATCCAGG CTGTAAAGGT AAGCCTGAAA GAGGAGCAGC GGCGCTCTGT GCACATACAG 
GAAGACAGAG AGGCTGTCGT GAACCAGTTA CAGATTCAAA TTCAGCAGTT ACAGAAGGAG 
CGGAATGACT ACTACTCAAA GATGCAGGTG CTAAAGAGCG AGTTGCAGGA GAATCAGACA 
AAGGCTGGCA AAATGGAGGC AGAACTGCAG AAAGCCAATA ACAAAGTGTG TAATATGGGT 
CACATGCTCA GCCAGCTATC TATCAAGCTG AACAATAGTG AAAACATGGA GCAGCAGATG 
GCTTTCCTGA ATAAGCAGCT GCTGCTTTTG GGTGAAGCCA ACAAGTTATA CATGGAAGAA 
ATTGACCGCT TGGGCCCTGA GGCGCACAAG GAGCTGAATA TGCTGCAAGC ATCTTCAGTG 
CGGGAGGGTG AGCGTTTGAG GCAGAGCTCT CTTCAGCAGA GTCAGAGACT TGAGGCTGCA 
CAGCAGCGTA TCACAGACCT GGAGAACCAA CTCACCAAGA AAGAGCAAGT CATCCGGGAG 
CAAAAGAAAC TGCTGGACAA TGTCAAACGA CAGGCCACGG AGCAGCTGCA GGCTTCAGAA 
AACAGGTATT TGGCTCAGAA GAACATCACT CAGGCATTAC AAACTGAGCT GCTTGAGCTC 
TATAGCAAGA TAGAGCTCAA AAACCTGAAA ACTAATGCAC CAGCAGAGTC CATTTCTGAT 
CCCAGCAGTC CCACCAACCA GAGGCCAAAT GGCAACAGTG CAGGCCCATC TGCTCCATCT 
CTACCGGCTG ACCTGATGAA AAGAGAGGAA GGGGCGCATG TGTGTGTCAA TGGTGAAATA 
TCTGCAGCGT CTCCTCAAAC ACCCACAGCA CTCATTAATG GCAGTCAGGA AGAAGATCTT 
TCCGCCCTTA GTCATTCATT TCCCTCCCTC CCCTGCCAAC CCTTTGCAGT TGGCTCTTAC 
CCCAGCACCA GAAGCTTTTT GGGTAAGAAA GCCAGAGAAA TGTTCCGCAA TAAGAGTGAG 
AGCCACTATG ATGGCGACTC GCCCACCCTC ACCTGCCTCT CAAAGGATCT TAAAGTTGAG 
CCAATCACAG ACCCCACAGA GAGTTTGGAG TCCGAGGAAG CTGCTGACCA GTTTGACCTG 
CAGCCAATGG ATAGACCCCG GGCATATAAT GTTCAGAGTA GGAGGCATCA GGATCTGCGG 
ATCATGGACT ATAATGAGAC CCATCATGTG CATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331694.1
CDS	478..2853
Translation	MKEELWSPSSICGMSTPPSSRGMSPTTVSDISLSASHLSGRIPSTPGDGKWTLSPTTPSGSSPLPSLSEEIGQAAQNTSSTQAKTCKEVKKVTSRSINNSSENGGDAKTPVSLAQLSDIVKRTEGEREDDTINEEILSLTHGKLDFGSRPGLESSFSGSLDTLLSSHPNHEQNLVSTPDRVECSAKAGEQQLPRSLWPFTPIENGKHSKCSLSDLTKESMPYEPLFDLALPKVASLFLERKTAEAFRRVEGERDHQDEMEGEDLSVTSPLEVLDRVIQQGHDTHGKVLKRTFSSPSQSSDPHLKGKQHSNKGKGLVPAVSDELDMLRSQLLLLHNQLLYERHKREQHALRNRRLFGRIVNTTALEEQNNSMKTQLKLQEVEIQAVKVSLKEEQRRSVHIQEDREAVVNQLQIQIQQLQKERNDYYSKMQVLKSELQENQTKAGKMEAELQKANNKVCNMGHMLSQLSIKLNNSENMEQQMAFLNKQLLLLGEANKLYMEEIDRLGPEAHKELNMLQASSVREGERLRQSSLQQSQRLEAAQQRITDLENQLTKKEQVIREQKKLLDNVKRQATEQLQASENRYLAQKNITQALQTELLELYSKIELKNLKTNAPAESISDPSSPTNQRPNGNSAGPSAPSLPADLMKREEGAHVCVNGEISAASPQTPTALINGSQEEDLSALSHSFPSLPCQPFAVGSYPSTRSFLGKKAREMFRNKSESHYDGDSPTLTCLSKDLKVEPITDPTESLESEEAADQFDLQPMDRPRAYNVQSRRHQDLRIMDYNETHHVH

Target ORF information:

RefSeq Version	XM_021476019.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tuberous sclerosis 1a (tsc1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476019.1

ORF Insert Sequence:

ATGAAGGAGG AGCTGTGGAG CCCTTCCTCC ATATGTGGGA TGAGCACCCC TCCCAGTTCT 
AGAGGAATGT CCCCAACCAC AGTTTCTGAC ATCTCACTTA GTGCCTCACA CCTCTCAGGA 
CGGATCCCCA GCACCCCAGG GGATGGAAAG TGGACTCTAT CTCCCACCAC ACCCTCGGGG 
TCATCCCCTC TCCCATCGCT CTCAGAGGAA ATAGGGCAGG CTGCCCAAAA CACCAGCAGT 
ACCCAGGCTA AGACGTGCAA AGAGGTGAAG AAAGTCACAT CCAGAAGTAT TAATAACAGC 
AGTGAGAATG GAGGTGATGC CAAGACTCCA GTGTCGTTGG CACAGCTCTC TGACATTGTC 
AAAAGGACAG AGGGGGAGCG AGAGGATGAT ACAATCAATG AGGAGATTTT GAGTCTAACC 
CATGGGAAAC TAGACTTTGG ATCTAGGCCT GGTCTGGAAT CCTCATTTTC TGGCTCCTTA 
GACACCCTTC TCTCATCTCA TCCTAATCAT GAGCAGAATT TGGTATCCAC ACCAGACAGG 
GTTGAATGCT CGGCTAAAGC AGGAGAGCAG CAGCTTCCTA GGTCTCTCTG GCCGTTCACC 
CCCATTGAAA ATGGGAAGCA CAGTAAATGT TCTCTGTCTG ATCTCACAAA AGAATCCATG 
CCTTATGAGC CACTGTTTGA CCTCGCCCTG CCGAAGGTGG CATCTCTGTT TTTGGAGAGG 
AAGACTGCTG AGGCCTTTCG GAGGGTTGAA GGAGAAAGAG ATCACCAAGA TGAGATGGAA 
GGAGAGGATC TTTCTGTCAC CTCCCCTCTG GAGGTGTTGG ACCGGGTTAT CCAGCAGGGT 
CACGACACTC ATGGGAAAGT GCTGAAGAGA ACTTTCTCTT CTCCAAGCCA ATCATCAGAC 
CCACACTTGA AAGGTAAACA GCATAGCAAT AAAGGAAAAG GTCTTGTGCC TGCAGTGTCG 
GATGAGCTAG ACATGTTACG CAGTCAATTG CTGCTGCTGC ATAACCAGTT GCTTTATGAG 
AGACATAAGA GAGAGCAACA TGCGCTTCGT AACCGTCGTC TGTTTGGCCG CATCGTCAAC 
ACTACTGCAC TGGAAGAGCA AAATAACTCC ATGAAAACGC AGCTCAAACT TCAGGAAGTT 
GAGATCCAGG CTGTAAAGGT AAGCCTGAAA GAGGAGCAGC GGCGCTCTGT GCACATACAG 
GAAGACAGAG AGGCTGTCGT GAACCAGTTA CAGATTCAAA TTCAGCAGTT ACAGAAGGAG 
CGGAATGACT ACTACTCAAA GATGCAGGTG CTAAAGAGCG AGTTGCAGGA GAATCAGACA 
AAGGCTGGCA AAATGGAGGC AGAACTGCAG AAAGCCAATA ACAAAGTGTG TAATATGGGT 
CACATGCTCA GCCAGCTATC TATCAAGCTG AACAATAGTG AAAACATGGA GCAGCAGATG 
GCTTTCCTGA ATAAGCAGCT GCTGCTTTTG GGTGAAGCCA ACAAGTTATA CATGGAAGAA 
ATTGACCGCT TGGGCCCTGA GGCGCACAAG GAGCTGAATA TGCTGCAAGC ATCTTCAGTG 
CGGGAGGGTG AGCGTTTGAG GCAGAGCTCT CTTCAGCAGA GTCAGAGACT TGAGGCTGCA 
CAGCAGCGTA TCACAGACCT GGAGAACCAA CTCACCAAGA AAGAGCAAGT CATCCGGGAG 
CAAAAGAAAC TGCTGGACAA TGTCAAACGA CAGGCCACGG AGCAGCTGCA GGCTTCAGAA 
AACAGGTATT TGGCTCAGAA GAACATCACT CAGGCATTAC AAACTGAGCT GCTTGAGCTC 
TATAGCAAGA TAGAGCTCAA AAACCTGAAA ACTAATGCAC CAGCAGAGTC CATTTCTGAT 
CCCAGCAGTC CCACCAACCA GAGGCCAAAT GGCAACAGTG CAGGCCCATC TGCTCCATCT 
CTACCGGCTG ACCTGATGAA AAGAGAGGAA GGGGCGCATG TGTGTGTCAA TGGTGAAATA 
TCTGCAGCGT CTCCTCAAAC ACCCACAGCA CTCATTAATG GCAGTCAGGA AGAAGATCTT 
TCCGCCCTTA GTCATTCATT TCCCTCCCTC CCCTGCCAAC CCTTTGCAGT TGGCTCTTAC 
CCCAGCACCA GAAGCTTTTT GGGTAAGAAA GCCAGAGAAA TGTTCCGCAA TAAGAGTGAG 
AGCCACTATG ATGGCGACTC GCCCACCCTC ACCTGCCTCT CAAAGGATCT TAAAGTTGAG 
CCAATCACAG ACCCCACAGA GAGTTTGGAG TCCGAGGAAG CTGCTGACCA GTTTGACCTG 
CAGCCAATGG ATAGACCCCG GGCATATAAT GTTCAGAGTA GGAGGCATCA GGATCTGCGG 
ATCATGGACT ATAATGAGAC CCATCATGTG CATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1A ( NM_200052.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_956346.1 Danio rerio TSC complex subunit 1a (tsc1a), mRNA.

Price & Availability↑

CloneID	ODa00014
Clone ID Related Accession (Same CDS sequence)	NM_200052.1
Accession Version	NM_200052.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-09
Organism	Danio rerio(zebrafish)
Product	hamartin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC055190.1. ##Evidence-Data-START## Transcript exon combination :: BC055190.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCCAAAG AGCAGCCAAG TGTGTTTGAC CTCATCCCTC TTCTGGGGTC CACTGACCTA 
CATGAGCTGG AACAGGTCAA AACCCTGCTG CAGGAAACTC TCAGTGCAGA TAAAGGGACC 
ATGTTGCTGA ATAGCCTTGT AGACTATTTC CTTCAGACAA ACTCATCTCA AGCTGTGGAC 
ATCCTGTCTT CTGTCCGGGA GCCTCATGAT AAGTATCTTT TAGATAAGAT GAATGAATGC 
ATGGGCAAGC AGAGCTGTCG TCTCTCCACC ATCACCCTCC TTGGTCATGT TGTTCGCAAA 
CAGCCACCAT GGATCCACAA GATCGCACGC TTCCCTCTGC TTGTCTCACT GCTCAAATGC 
CTCAAGACTG ACTCAGATGT AGTAGTTCTC ATAACCGGTG TGCTGGTGCT GATCACTCTA 
ATGCCCATGA TTCCTCAAAC CAACAAACAA CTTCTTTGCG ACTACTTTGA TATCTTTGGA 
CGTCTTGCTG CCTGGAACCA ACGTCATCCA GGCCATGCTC AGGGAGTGTT TGCAGTCCAT 
CTACATGCCA GCGTGTACTC TCTTTTCCAT CGTTTATATG GCATGTATCC TTGTAATTTC 
GTTTCGTACC TGCGTGCCTA TTACAGTATG AAGGAAAATG TTGACACGTT CGAGGAAGTG 
GTGAAGCCCA TGTTGGAACA TGTTCGGATA CATCCTGAGC TCATCACTGG AACCAAAGAC 
TACGAGGTTG ATCCAATTAG ATGGAAGAGA TTTGAGCCAC ATGATATTGT GATTGAATGT 
GCCAAAATTT CACTGGATTC TAAAGAGGCA TCATCTGAAG AGGGCTACTC CTCTTTGGCG 
GATCACATAC AGCGACGCGC TGTACACCAT AGCCAATCAT GTTCAGAGCT CAGTTTCCAT 
GAAATCTCAT CAGTCACACC AATAGGTGCT ATTTGTCAGT CTCTCTCTAA ATCACAACCT 
TATCTCACTG TAACCCAAGA CTCAGGTTCC AGCGCACAGA CCCCCAACAA TCAAAGTGAT 
GAGGTCAAAT CCTCAGGGAT GAAGGAGGAG CTGTGGAGCC CTTCCTCGAT ATGTGGGATG 
AGCACCCCTC CCAGTTCTAG AGGAATGTCC CCAACCACAG TTTCTGACAT CTCACTTAGT 
GCCTCACACC TCTCAGGACG GATCCCCAGC ACCCCAGGGG ATGGAAAGTG GACTCTATCT 
CCCTCCACAC CCTCGGGGTC ATCCCCTCTC CCATCGCTCT CAGAGGAAAT AGGGCAGGCT 
GCCCAAAACA CCAGCAGTAC CCAGGCTAAG ACGTGCGAAG AGGTGAAGAA AGTCACATCC 
AGAAGTATTA ATAACAGCAG TGAGAATGGA GGTGATGCCA AGACTCCAGT GTCGTTGGCA 
CAGCTCTCTG ACATTGTCAA AAGGACAGAG GGGGAGCGAG AGGATGATAC AATCAATGAG 
GAGATTTTAA GTCTAACACA TGGGAAACTA GACTTTGGAT CTAGGCCTGG TCTGGAATCC 
TCATTTTCTG GCTCCTTAGA CACCCTTCTC TCATCTCATC CCAACCATGA GCAGAATTTG 
GTATCCACAC CAGACAGGGT TGAATGCTCG GCTAAAGCAG GAGAGCACCA GCTTCCTAGG 
TCTCTCTGGC CGTTCACCCC CATTGAAAAT GGGAAGCACA GTAAATGTTC TCTGTCTGAT 
CTCACAAAAG AATCCATGCC TTATGAGCCG CTGTTTGACC TCGCCCTGCC GAAGGTGGCA 
TCTCTGTTTT TGGAGAGGAA GACTGCTGAG GCCTTTCGGA GGGTTGAAGG AGAAAGAGAT 
CACCAAGATG AGATGGAAGG AGAGGATCTT TCTGTCACCT CCCCTCTGGA GGTGTTGGAC 
CGGGTTATCC AGCAGGGTCA CGACACCCAT GGGAAAGTGC TGAAGAGAAC TTTCTCTTCT 
CCAAGCCAAT CATCAGACCC ACACTTGAAA GGTCTTGTGC CTGCAGTGTC GGATGAGCTA 
GACATGTTAC GCAGTCAATT GCTGCTGCTG CATAACCAGT TGCTTTATGA GAGACATAAG 
AGAGAGCAAC ATGCGCTTCG TAACCGTCGT CTGTTTGGCC GCATCGTCAA CACTACTGCA 
CTGGAAGAGC AAAATAACTC CATGAAAACG CAGCTCAAAC TTCAGGAAGT TGAGATCCAG 
GCTGTAAAGG TAAGCCTGAA AGAGGAGCAG CGGCGCTCTG TGCACATACA GGAAGACAGA 
GAGGCTGTCG TGAACCAGTT ACAGACTCAA ATTCAGCAGT TACAGAAGGA GCGGAATGAC 
TACTACTCAA AGATGCAGGT GCTAAAGAGC GAGTTGCAGG AGAATCAGAC AAAGGCTGGC 
AAAATGGAGG CAGAACTGCA GAAAGCCAAT AACAAAGTGT GTAATATGGG TCACATGCTC 
AGCCAGCTAT CTATCAAGCT GAACAATAGC GAAAACATGG AGCAGCAGAT GGCTTTCCTG 
AATAAGCAGC TGCTGCTTTT GGGTGAAGCC AACAAGTTAT ACATGGAAGA AATTGACCGC 
TTGGGCCCTG AGGCGCACAA GGAGCTGAAT ATGCTGCAAG CATCTTCAGT GCGGGAGGGT 
GAGCGTTTGA GGCAGAGCTC TCTTCAGCAG AGTCAGAGAC TTGAGGCTGC ACAGCAGCGC 
ATCACAGACC TGGAGAACCA ACTCACCAAG AAAGAGCAAG TCATTCGGGA GCAAAAGAAA 
CTGCTGGACA ATGTCAAACG ACAGGCCACG GAGCAGCTGC AGGCTTCAGA AAACAGGTAT 
TTGGCTCAGA AGAACATCAC TCAGGCATTA CAAACTGAGC TGCTTGAGCT CTATAGCAAG 
ATAGAGCTCA AAAACCTGAA AACTAATGCA CCAGCAGAGC CCATTTCTGA TCCCAGCAGT 
CCCACCAACC AGAGGCCAAA TGGCAACAGT GCAGGCCCAT CTGCTCCCTC TCTACCGGCT 
GACCTGATGA AAAGAGAGGA AGGGGCGCAT GTGTGTGTCA ATGGTGAAAT ATCTGCAGCG 
TCTCCTCAAA CACCCACAGC ACTCATTAAT GGCAGTCAGG AAGAAGATCT TTCCGCCCTT 
ACTCATTCAT TTCCCTCCCT CCCCTGCCAA CCCTTTGCAG TTGGCTCTTA CCCCAGCACC 
AGAAGCTTTT TGGGTAAGAA AGCCAGAGAA ATGTTCCGCA ATAAGAGTGA GAGCCACTAT 
GATGGCGACT CGCCCACCCT CACCTGCCTC TCAAAGGATC TTAAAGTTGA GCCAATCACA 
GACCCCACAG AGAGTTTGGA GTCCGAGGAA GCTGCTGACC AGTTTGACCT GCAGCCAATG 
GATAGACCCC GGGCATATAA TGTTCAGAGT AGGAGGCATC AGGATCTGCG GATCATGGAC 
TATAATGAGA CCCACCATGT GCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956346.1
CDS	151..3537
Translation	MAKEQPSVFDLIPLLGSTDLHELEQVKTLLQETLSADKGTMLLNSLVDYFLQTNSSQAVDILSSVREPHDKYLLDKMNECMGKQSCRLSTITLLGHVVRKQPPWIHKIARFPLLVSLLKCLKTDSDVVVLITGVLVLITLMPMIPQTNKQLLCDYFDIFGRLAAWNQRHPGHAQGVFAVHLHASVYSLFHRLYGMYPCNFVSYLRAYYSMKENVDTFEEVVKPMLEHVRIHPELITGTKDYEVDPIRWKRFEPHDIVIECAKISLDSKEASSEEGYSSLADHIQRRAVHHSQSCSELSFHEISSVTPIGAICQSLSKSQPYLTVTQDSGSSAQTPNNQSDEVKSSGMKEELWSPSSICGMSTPPSSRGMSPTTVSDISLSASHLSGRIPSTPGDGKWTLSPSTPSGSSPLPSLSEEIGQAAQNTSSTQAKTCEEVKKVTSRSINNSSENGGDAKTPVSLAQLSDIVKRTEGEREDDTINEEILSLTHGKLDFGSRPGLESSFSGSLDTLLSSHPNHEQNLVSTPDRVECSAKAGEHQLPRSLWPFTPIENGKHSKCSLSDLTKESMPYEPLFDLALPKVASLFLERKTAEAFRRVEGERDHQDEMEGEDLSVTSPLEVLDRVIQQGHDTHGKVLKRTFSSPSQSSDPHLKGLVPAVSDELDMLRSQLLLLHNQLLYERHKREQHALRNRRLFGRIVNTTALEEQNNSMKTQLKLQEVEIQAVKVSLKEEQRRSVHIQEDREAVVNQLQTQIQQLQKERNDYYSKMQVLKSELQENQTKAGKMEAELQKANNKVCNMGHMLSQLSIKLNNSENMEQQMAFLNKQLLLLGEANKLYMEEIDRLGPEAHKELNMLQASSVREGERLRQSSLQQSQRLEAAQQRITDLENQLTKKEQVIREQKKLLDNVKRQATEQLQASENRYLAQKNITQALQTELLELYSKIELKNLKTNAPAEPISDPSSPTNQRPNGNSAGPSAPSLPADLMKREEGAHVCVNGEISAASPQTPTALINGSQEEDLSALTHSFPSLPCQPFAVGSYPSTRSFLGKKAREMFRNKSESHYDGDSPTLTCLSKDLKVEPITDPTESLESEEAADQFDLQPMDRPRAYNVQSRRHQDLRIMDYNETHHVH

Target ORF information:

RefSeq Version	NM_200052.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TSC complex subunit 1a (tsc1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200052.1

ORF Insert Sequence:

ATGGCCAAAG AGCAGCCAAG TGTGTTTGAC CTCATCCCTC TTCTGGGGTC CACTGACCTA 
CATGAGCTGG AACAGGTCAA AACCCTGCTG CAGGAAACTC TCAGTGCAGA TAAAGGGACC 
ATGTTGCTGA ATAGCCTTGT AGACTATTTC CTTCAGACAA ACTCATCTCA AGCTGTGGAC 
ATCCTGTCTT CTGTCCGGGA GCCTCATGAT AAGTATCTTT TAGATAAGAT GAATGAATGC 
ATGGGCAAGC AGAGCTGTCG TCTCTCCACC ATCACCCTCC TTGGTCATGT TGTTCGCAAA 
CAGCCACCAT GGATCCACAA GATCGCACGC TTCCCTCTGC TTGTCTCACT GCTCAAATGC 
CTCAAGACTG ACTCAGATGT AGTAGTTCTC ATAACCGGTG TGCTGGTGCT GATCACTCTA 
ATGCCCATGA TTCCTCAAAC CAACAAACAA CTTCTTTGCG ACTACTTTGA TATCTTTGGA 
CGTCTTGCTG CCTGGAACCA ACGTCATCCA GGCCATGCTC AGGGAGTGTT TGCAGTCCAT 
CTACATGCCA GCGTGTACTC TCTTTTCCAT CGTTTATATG GCATGTATCC TTGTAATTTC 
GTTTCGTACC TGCGTGCCTA TTACAGTATG AAGGAAAATG TTGACACGTT CGAGGAAGTG 
GTGAAGCCCA TGTTGGAACA TGTTCGGATA CATCCTGAGC TCATCACTGG AACCAAAGAC 
TACGAGGTTG ATCCAATTAG ATGGAAGAGA TTTGAGCCAC ATGATATTGT GATTGAATGT 
GCCAAAATTT CACTGGATTC TAAAGAGGCA TCATCTGAAG AGGGCTACTC CTCTTTGGCG 
GATCACATAC AGCGACGCGC TGTACACCAT AGCCAATCAT GTTCAGAGCT CAGTTTCCAT 
GAAATCTCAT CAGTCACACC AATAGGTGCT ATTTGTCAGT CTCTCTCTAA ATCACAACCT 
TATCTCACTG TAACCCAAGA CTCAGGTTCC AGCGCACAGA CCCCCAACAA TCAAAGTGAT 
GAGGTCAAAT CCTCAGGGAT GAAGGAGGAG CTGTGGAGCC CTTCCTCGAT ATGTGGGATG 
AGCACCCCTC CCAGTTCTAG AGGAATGTCC CCAACCACAG TTTCTGACAT CTCACTTAGT 
GCCTCACACC TCTCAGGACG GATCCCCAGC ACCCCAGGGG ATGGAAAGTG GACTCTATCT 
CCCTCCACAC CCTCGGGGTC ATCCCCTCTC CCATCGCTCT CAGAGGAAAT AGGGCAGGCT 
GCCCAAAACA CCAGCAGTAC CCAGGCTAAG ACGTGCGAAG AGGTGAAGAA AGTCACATCC 
AGAAGTATTA ATAACAGCAG TGAGAATGGA GGTGATGCCA AGACTCCAGT GTCGTTGGCA 
CAGCTCTCTG ACATTGTCAA AAGGACAGAG GGGGAGCGAG AGGATGATAC AATCAATGAG 
GAGATTTTAA GTCTAACACA TGGGAAACTA GACTTTGGAT CTAGGCCTGG TCTGGAATCC 
TCATTTTCTG GCTCCTTAGA CACCCTTCTC TCATCTCATC CCAACCATGA GCAGAATTTG 
GTATCCACAC CAGACAGGGT TGAATGCTCG GCTAAAGCAG GAGAGCACCA GCTTCCTAGG 
TCTCTCTGGC CGTTCACCCC CATTGAAAAT GGGAAGCACA GTAAATGTTC TCTGTCTGAT 
CTCACAAAAG AATCCATGCC TTATGAGCCG CTGTTTGACC TCGCCCTGCC GAAGGTGGCA 
TCTCTGTTTT TGGAGAGGAA GACTGCTGAG GCCTTTCGGA GGGTTGAAGG AGAAAGAGAT 
CACCAAGATG AGATGGAAGG AGAGGATCTT TCTGTCACCT CCCCTCTGGA GGTGTTGGAC 
CGGGTTATCC AGCAGGGTCA CGACACCCAT GGGAAAGTGC TGAAGAGAAC TTTCTCTTCT 
CCAAGCCAAT CATCAGACCC ACACTTGAAA GGTCTTGTGC CTGCAGTGTC GGATGAGCTA 
GACATGTTAC GCAGTCAATT GCTGCTGCTG CATAACCAGT TGCTTTATGA GAGACATAAG 
AGAGAGCAAC ATGCGCTTCG TAACCGTCGT CTGTTTGGCC GCATCGTCAA CACTACTGCA 
CTGGAAGAGC AAAATAACTC CATGAAAACG CAGCTCAAAC TTCAGGAAGT TGAGATCCAG 
GCTGTAAAGG TAAGCCTGAA AGAGGAGCAG CGGCGCTCTG TGCACATACA GGAAGACAGA 
GAGGCTGTCG TGAACCAGTT ACAGACTCAA ATTCAGCAGT TACAGAAGGA GCGGAATGAC 
TACTACTCAA AGATGCAGGT GCTAAAGAGC GAGTTGCAGG AGAATCAGAC AAAGGCTGGC 
AAAATGGAGG CAGAACTGCA GAAAGCCAAT AACAAAGTGT GTAATATGGG TCACATGCTC 
AGCCAGCTAT CTATCAAGCT GAACAATAGC GAAAACATGG AGCAGCAGAT GGCTTTCCTG 
AATAAGCAGC TGCTGCTTTT GGGTGAAGCC AACAAGTTAT ACATGGAAGA AATTGACCGC 
TTGGGCCCTG AGGCGCACAA GGAGCTGAAT ATGCTGCAAG CATCTTCAGT GCGGGAGGGT 
GAGCGTTTGA GGCAGAGCTC TCTTCAGCAG AGTCAGAGAC TTGAGGCTGC ACAGCAGCGC 
ATCACAGACC TGGAGAACCA ACTCACCAAG AAAGAGCAAG TCATTCGGGA GCAAAAGAAA 
CTGCTGGACA ATGTCAAACG ACAGGCCACG GAGCAGCTGC AGGCTTCAGA AAACAGGTAT 
TTGGCTCAGA AGAACATCAC TCAGGCATTA CAAACTGAGC TGCTTGAGCT CTATAGCAAG 
ATAGAGCTCA AAAACCTGAA AACTAATGCA CCAGCAGAGC CCATTTCTGA TCCCAGCAGT 
CCCACCAACC AGAGGCCAAA TGGCAACAGT GCAGGCCCAT CTGCTCCCTC TCTACCGGCT 
GACCTGATGA AAAGAGAGGA AGGGGCGCAT GTGTGTGTCA ATGGTGAAAT ATCTGCAGCG 
TCTCCTCAAA CACCCACAGC ACTCATTAAT GGCAGTCAGG AAGAAGATCT TTCCGCCCTT 
ACTCATTCAT TTCCCTCCCT CCCCTGCCAA CCCTTTGCAG TTGGCTCTTA CCCCAGCACC 
AGAAGCTTTT TGGGTAAGAA AGCCAGAGAA ATGTTCCGCA ATAAGAGTGA GAGCCACTAT 
GATGGCGACT CGCCCACCCT CACCTGCCTC TCAAAGGATC TTAAAGTTGA GCCAATCACA 
GACCCCACAG AGAGTTTGGA GTCCGAGGAA GCTGCTGACC AGTTTGACCT GCAGCCAATG 
GATAGACCCC GGGCATATAA TGTTCAGAGT AGGAGGCATC AGGATCTGCG GATCATGGAC 
TATAATGAGA CCCACCATGT GCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1A ( XM_005171795.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005171852.1 Danio rerio tuberous sclerosis 1a (tsc1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10423
Clone ID Related Accession (Same CDS sequence)	XM_005171795.4 , XM_005171796.3 , XM_005171796.4 , XM_005171795.3
Accession Version	XM_005171795.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171795.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG AGCAGCCAAG TGTGTTTGAC CTCATCCCTC TTCTGGGGTC CACTGACCTA 
CATGAGCTGG AACAGGTCAA AACCTTGCTG CAGGAAACTC TCAGTGCAGA TAAAGGGACC 
ATGTTGCTGA ATAGCCTTGT AGACTATTTC CTTCAGACAA ACTCATCTCA AGCTGTGGAC 
ATCCTGTCTT CTGTCCGGGA GCCTCATGAT AAGTATCTTT TAGATAAGAT GAATGAATGC 
ATGGGCAAGC AGAGCTGTCG TCTCTCCACC ATCACCCTCC TTGGTCATGT TGTTCGCAAA 
CAGCCACCAT GGATCCACAA GATCGCACGC TTCCCTCTGC TTGTCTCGCT GCTCAAATGC 
CTCAAGACTG ACTCAGATGT AGTAGTTCTC ATAACTGGTG TGCTGGTGCT GATCACTCTA 
ATGCCCATGA TTCCTCAAAC CAACAAACAA CTTCTTTGCG ACTACTTTGA TATCTTTGGA 
CGTCTTGCTG CCTGGAACCA ACGTCATCCA GGCCATGCTC AGGGAGTGTT TGCAGTCCAT 
CTACATGCCA GCGTGTACTC TCTTTTCCAT CGTTTATATG GCATGTATCC TTGTAATTTC 
GTTTCGTACC TGCGTGCCCA TTACAGTATG AAGGAAAATG TTGACACGTT CGAGGAAGTG 
GTGAAGCCCA TGTTGGAACA TGTTCGGATA CATCCTGAGC TCATCACTGG AACCAAAGAC 
TACGAGGTTG ATCCAATTAG ATGGAAGAGA TTTGAGCCAC ATGATATTGT GATTGAATGT 
GCCAAAATTT CACTGGATTC TAAAGAGGCG TCATCTGAAG AGGGCTACTC CTCTTTGGCG 
GATCACATAC AGCGACGCGC TGTACACCAT AGCCAATCAT GTTCAGAGCT CAGTTTCCAT 
GAAATCTCAT CAGTCACACC AATAGGTGCT ATTTGTCAGT CTCTCTCTAA ATCACAACCT 
TATCTCACTG TAACCCAAGA CTCAGGTTCC AGCGCACAGA CCCTCAACAA TCAAAGTGAT 
GAGGTCAAAT CCTCAGGGAT GAAGGAGGAG CTGTGGAGCC CTTCCTCCAT ATGTGGGATG 
AGCACCCCTC CCAGTTCTAG AGGAATGTCC CCAACCACAG TTTCTGACAT CTCACTTAGT 
GCCTCACACC TCTCAGGACG GATCCCCAGC ACCCCAGGGG ATGGAAAGTG GACTCTATCT 
CCCACCACAC CCTCGGGGTC ATCCCCTCTC CCATCGCTCT CAGAGGAAAT AGGGCAGGCT 
GCCCAAAACA CCAGCAGTAC CCAGGCTAAG ACGTGCAAAG AGGTGAAGAA AGTCACATCC 
AGAAGTATTA ATAACAGCAG TGAGAATGGA GGTGATGCCA AGACTCCAGT GTCGTTGGCA 
CAGCTCTCTG ACATTGTCAA AAGGACAGAG GGGGAGCGAG AGGATGATAC AATCAATGAG 
GAGATTTTGA GTCTAACCCA TGGGAAACTA GACTTTGGAT CTAGGCCTGG TCTGGAATCC 
TCATTTTCTG GCTCCTTAGA CACCCTTCTC TCATCTCATC CTAATCATGA GCAGAATTTG 
GTATCCACAC CAGACAGGGT TGAATGCTCG GCTAAAGCAG GAGAGCAGCA GCTTCCTAGG 
TCTCTCTGGC CGTTCACCCC CATTGAAAAT GGGAAGCACA GTAAATGTTC TCTGTCTGAT 
CTCACAAAAG AATCCATGCC TTATGAGCCA CTGTTTGACC TCGCCCTGCC GAAGGTGGCA 
TCTCTGTTTT TGGAGAGGAA GACTGCTGAG GCCTTTCGGA GGGTTGAAGG AGAAAGAGAT 
CACCAAGATG AGATGGAAGG AGAGGATCTT TCTGTCACCT CCCCTCTGGA GGTGTTGGAC 
CGGGTTATCC AGCAGGGTCA CGACACTCAT GGGAAAGTGC TGAAGAGAAC TTTCTCTTCT 
CCAAGCCAAT CATCAGACCC ACACTTGAAA GGTAAACAGC ATAGCAATAA AGGAAAAGGT 
CTTGTGCCTG CAGTGTCGGA TGAGCTAGAC ATGTTACGCA GTCAATTGCT GCTGCTGCAT 
AACCAGTTGC TTTATGAGAG ACATAAGAGA GAGCAACATG CGCTTCGTAA CCGTCGTCTG 
TTTGGCCGCA TCGTCAACAC TACTGCACTG GAAGAGCAAA ATAACTCCAT GAAAACGCAG 
CTCAAACTTC AGGAAGTTGA GATCCAGGCT GTAAAGGTAA GCCTGAAAGA GGAGCAGCGG 
CGCTCTGTGC ACATACAGGA AGACAGAGAG GCTGTCGTGA ACCAGTTACA GATTCAAATT 
CAGCAGTTAC AGAAGGAGCG GAATGACTAC TACTCAAAGA TGCAGGTGCT AAAGAGCGAG 
TTGCAGGAGA ATCAGACAAA GGCTGGCAAA ATGGAGGCAG AACTGCAGAA AGCCAATAAC 
AAAGTGTGTA ATATGGGTCA CATGCTCAGC CAGCTATCTA TCAAGCTGAA CAATAGTGAA 
AACATGGAGC AGCAGATGGC TTTCCTGAAT AAGCAGCTGC TGCTTTTGGG TGAAGCCAAC 
AAGTTATACA TGGAAGAAAT TGACCGCTTG GGCCCTGAGG CGCACAAGGA GCTGAATATG 
CTGCAAGCAT CTTCAGTGCG GGAGGGTGAG CGTTTGAGGC AGAGCTCTCT TCAGCAGAGT 
CAGAGACTTG AGGCTGCACA GCAGCGTATC ACAGACCTGG AGAACCAACT CACCAAGAAA 
GAGCAAGTCA TCCGGGAGCA AAAGAAACTG CTGGACAATG TCAAACGACA GGCCACGGAG 
CAGCTGCAGG CTTCAGAAAA CAGGTATTTG GCTCAGAAGA ACATCACTCA GGCATTACAA 
ACTGAGCTGC TTGAGCTCTA TAGCAAGATA GAGCTCAAAA ACCTGAAAAC TAATGCACCA 
GCAGAGTCCA TTTCTGATCC CAGCAGTCCC ACCAACCAGA GGCCAAATGG CAACAGTGCA 
GGCCCATCTG CTCCATCTCT ACCGGCTGAC CTGATGAAAA GAGAGGAAGG GGCGCATGTG 
TGTGTCAATG GTGAAATATC TGCAGCGTCT CCTCAAACAC CCACAGCACT CATTAATGGC 
AGTCAGGAAG AAGATCTTTC CGCCCTTAGT CATTCATTTC CCTCCCTCCC CTGCCAACCC 
TTTGCAGTTG GCTCTTACCC CAGCACCAGA AGCTTTTTGG GTAAGAAAGC CAGAGAAATG 
TTCCGCAATA AGAGTGAGAG CCACTATGAT GGCGACTCGC CCACCCTCAC CTGCCTCTCA 
AAGGATCTTA AAGTTGAGCC AATCACAGAC CCCACAGAGA GTTTGGAGTC CGAGGAAGCT 
GCTGACCAGT TTGACCTGCA GCCAATGGAT AGACCCCGGG CATATAATGT TCAGAGTAGG 
AGGCATCAGG ATCTGCGGAT CATGGACTAT AATGAGACCC ATCATGTGCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171852.1
CDS	222..3635
Translation	MAKEQPSVFDLIPLLGSTDLHELEQVKTLLQETLSADKGTMLLNSLVDYFLQTNSSQAVDILSSVREPHDKYLLDKMNECMGKQSCRLSTITLLGHVVRKQPPWIHKIARFPLLVSLLKCLKTDSDVVVLITGVLVLITLMPMIPQTNKQLLCDYFDIFGRLAAWNQRHPGHAQGVFAVHLHASVYSLFHRLYGMYPCNFVSYLRAHYSMKENVDTFEEVVKPMLEHVRIHPELITGTKDYEVDPIRWKRFEPHDIVIECAKISLDSKEASSEEGYSSLADHIQRRAVHHSQSCSELSFHEISSVTPIGAICQSLSKSQPYLTVTQDSGSSAQTLNNQSDEVKSSGMKEELWSPSSICGMSTPPSSRGMSPTTVSDISLSASHLSGRIPSTPGDGKWTLSPTTPSGSSPLPSLSEEIGQAAQNTSSTQAKTCKEVKKVTSRSINNSSENGGDAKTPVSLAQLSDIVKRTEGEREDDTINEEILSLTHGKLDFGSRPGLESSFSGSLDTLLSSHPNHEQNLVSTPDRVECSAKAGEQQLPRSLWPFTPIENGKHSKCSLSDLTKESMPYEPLFDLALPKVASLFLERKTAEAFRRVEGERDHQDEMEGEDLSVTSPLEVLDRVIQQGHDTHGKVLKRTFSSPSQSSDPHLKGKQHSNKGKGLVPAVSDELDMLRSQLLLLHNQLLYERHKREQHALRNRRLFGRIVNTTALEEQNNSMKTQLKLQEVEIQAVKVSLKEEQRRSVHIQEDREAVVNQLQIQIQQLQKERNDYYSKMQVLKSELQENQTKAGKMEAELQKANNKVCNMGHMLSQLSIKLNNSENMEQQMAFLNKQLLLLGEANKLYMEEIDRLGPEAHKELNMLQASSVREGERLRQSSLQQSQRLEAAQQRITDLENQLTKKEQVIREQKKLLDNVKRQATEQLQASENRYLAQKNITQALQTELLELYSKIELKNLKTNAPAESISDPSSPTNQRPNGNSAGPSAPSLPADLMKREEGAHVCVNGEISAASPQTPTALINGSQEEDLSALSHSFPSLPCQPFAVGSYPSTRSFLGKKAREMFRNKSESHYDGDSPTLTCLSKDLKVEPITDPTESLESEEAADQFDLQPMDRPRAYNVQSRRHQDLRIMDYNETHHVH

Target ORF information:

RefSeq Version	XM_005171795.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tuberous sclerosis 1a (tsc1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171795.4

ORF Insert Sequence:

ATGGCCAAAG AGCAGCCAAG TGTGTTTGAC CTCATCCCTC TTCTGGGGTC CACTGACCTA 
CATGAGCTGG AACAGGTCAA AACCTTGCTG CAGGAAACTC TCAGTGCAGA TAAAGGGACC 
ATGTTGCTGA ATAGCCTTGT AGACTATTTC CTTCAGACAA ACTCATCTCA AGCTGTGGAC 
ATCCTGTCTT CTGTCCGGGA GCCTCATGAT AAGTATCTTT TAGATAAGAT GAATGAATGC 
ATGGGCAAGC AGAGCTGTCG TCTCTCCACC ATCACCCTCC TTGGTCATGT TGTTCGCAAA 
CAGCCACCAT GGATCCACAA GATCGCACGC TTCCCTCTGC TTGTCTCGCT GCTCAAATGC 
CTCAAGACTG ACTCAGATGT AGTAGTTCTC ATAACTGGTG TGCTGGTGCT GATCACTCTA 
ATGCCCATGA TTCCTCAAAC CAACAAACAA CTTCTTTGCG ACTACTTTGA TATCTTTGGA 
CGTCTTGCTG CCTGGAACCA ACGTCATCCA GGCCATGCTC AGGGAGTGTT TGCAGTCCAT 
CTACATGCCA GCGTGTACTC TCTTTTCCAT CGTTTATATG GCATGTATCC TTGTAATTTC 
GTTTCGTACC TGCGTGCCCA TTACAGTATG AAGGAAAATG TTGACACGTT CGAGGAAGTG 
GTGAAGCCCA TGTTGGAACA TGTTCGGATA CATCCTGAGC TCATCACTGG AACCAAAGAC 
TACGAGGTTG ATCCAATTAG ATGGAAGAGA TTTGAGCCAC ATGATATTGT GATTGAATGT 
GCCAAAATTT CACTGGATTC TAAAGAGGCG TCATCTGAAG AGGGCTACTC CTCTTTGGCG 
GATCACATAC AGCGACGCGC TGTACACCAT AGCCAATCAT GTTCAGAGCT CAGTTTCCAT 
GAAATCTCAT CAGTCACACC AATAGGTGCT ATTTGTCAGT CTCTCTCTAA ATCACAACCT 
TATCTCACTG TAACCCAAGA CTCAGGTTCC AGCGCACAGA CCCTCAACAA TCAAAGTGAT 
GAGGTCAAAT CCTCAGGGAT GAAGGAGGAG CTGTGGAGCC CTTCCTCCAT ATGTGGGATG 
AGCACCCCTC CCAGTTCTAG AGGAATGTCC CCAACCACAG TTTCTGACAT CTCACTTAGT 
GCCTCACACC TCTCAGGACG GATCCCCAGC ACCCCAGGGG ATGGAAAGTG GACTCTATCT 
CCCACCACAC CCTCGGGGTC ATCCCCTCTC CCATCGCTCT CAGAGGAAAT AGGGCAGGCT 
GCCCAAAACA CCAGCAGTAC CCAGGCTAAG ACGTGCAAAG AGGTGAAGAA AGTCACATCC 
AGAAGTATTA ATAACAGCAG TGAGAATGGA GGTGATGCCA AGACTCCAGT GTCGTTGGCA 
CAGCTCTCTG ACATTGTCAA AAGGACAGAG GGGGAGCGAG AGGATGATAC AATCAATGAG 
GAGATTTTGA GTCTAACCCA TGGGAAACTA GACTTTGGAT CTAGGCCTGG TCTGGAATCC 
TCATTTTCTG GCTCCTTAGA CACCCTTCTC TCATCTCATC CTAATCATGA GCAGAATTTG 
GTATCCACAC CAGACAGGGT TGAATGCTCG GCTAAAGCAG GAGAGCAGCA GCTTCCTAGG 
TCTCTCTGGC CGTTCACCCC CATTGAAAAT GGGAAGCACA GTAAATGTTC TCTGTCTGAT 
CTCACAAAAG AATCCATGCC TTATGAGCCA CTGTTTGACC TCGCCCTGCC GAAGGTGGCA 
TCTCTGTTTT TGGAGAGGAA GACTGCTGAG GCCTTTCGGA GGGTTGAAGG AGAAAGAGAT 
CACCAAGATG AGATGGAAGG AGAGGATCTT TCTGTCACCT CCCCTCTGGA GGTGTTGGAC 
CGGGTTATCC AGCAGGGTCA CGACACTCAT GGGAAAGTGC TGAAGAGAAC TTTCTCTTCT 
CCAAGCCAAT CATCAGACCC ACACTTGAAA GGTAAACAGC ATAGCAATAA AGGAAAAGGT 
CTTGTGCCTG CAGTGTCGGA TGAGCTAGAC ATGTTACGCA GTCAATTGCT GCTGCTGCAT 
AACCAGTTGC TTTATGAGAG ACATAAGAGA GAGCAACATG CGCTTCGTAA CCGTCGTCTG 
TTTGGCCGCA TCGTCAACAC TACTGCACTG GAAGAGCAAA ATAACTCCAT GAAAACGCAG 
CTCAAACTTC AGGAAGTTGA GATCCAGGCT GTAAAGGTAA GCCTGAAAGA GGAGCAGCGG 
CGCTCTGTGC ACATACAGGA AGACAGAGAG GCTGTCGTGA ACCAGTTACA GATTCAAATT 
CAGCAGTTAC AGAAGGAGCG GAATGACTAC TACTCAAAGA TGCAGGTGCT AAAGAGCGAG 
TTGCAGGAGA ATCAGACAAA GGCTGGCAAA ATGGAGGCAG AACTGCAGAA AGCCAATAAC 
AAAGTGTGTA ATATGGGTCA CATGCTCAGC CAGCTATCTA TCAAGCTGAA CAATAGTGAA 
AACATGGAGC AGCAGATGGC TTTCCTGAAT AAGCAGCTGC TGCTTTTGGG TGAAGCCAAC 
AAGTTATACA TGGAAGAAAT TGACCGCTTG GGCCCTGAGG CGCACAAGGA GCTGAATATG 
CTGCAAGCAT CTTCAGTGCG GGAGGGTGAG CGTTTGAGGC AGAGCTCTCT TCAGCAGAGT 
CAGAGACTTG AGGCTGCACA GCAGCGTATC ACAGACCTGG AGAACCAACT CACCAAGAAA 
GAGCAAGTCA TCCGGGAGCA AAAGAAACTG CTGGACAATG TCAAACGACA GGCCACGGAG 
CAGCTGCAGG CTTCAGAAAA CAGGTATTTG GCTCAGAAGA ACATCACTCA GGCATTACAA 
ACTGAGCTGC TTGAGCTCTA TAGCAAGATA GAGCTCAAAA ACCTGAAAAC TAATGCACCA 
GCAGAGTCCA TTTCTGATCC CAGCAGTCCC ACCAACCAGA GGCCAAATGG CAACAGTGCA 
GGCCCATCTG CTCCATCTCT ACCGGCTGAC CTGATGAAAA GAGAGGAAGG GGCGCATGTG 
TGTGTCAATG GTGAAATATC TGCAGCGTCT CCTCAAACAC CCACAGCACT CATTAATGGC 
AGTCAGGAAG AAGATCTTTC CGCCCTTAGT CATTCATTTC CCTCCCTCCC CTGCCAACCC 
TTTGCAGTTG GCTCTTACCC CAGCACCAGA AGCTTTTTGG GTAAGAAAGC CAGAGAAATG 
TTCCGCAATA AGAGTGAGAG CCACTATGAT GGCGACTCGC CCACCCTCAC CTGCCTCTCA 
AAGGATCTTA AAGTTGAGCC AATCACAGAC CCCACAGAGA GTTTGGAGTC CGAGGAAGCT 
GCTGACCAGT TTGACCTGCA GCCAATGGAT AGACCCCGGG CATATAATGT TCAGAGTAGG 
AGGCATCAGG ATCTGCGGAT CATGGACTAT AATGAGACCC ATCATGTGCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1A ( XM_005171796.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005171853.1 Danio rerio tuberous sclerosis 1a (tsc1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa10423
Clone ID Related Accession (Same CDS sequence)	XM_005171795.4 , XM_005171796.3 , XM_005171796.4 , XM_005171795.3
Accession Version	XM_005171796.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	hamartin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171796.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG AGCAGCCAAG TGTGTTTGAC CTCATCCCTC TTCTGGGGTC CACTGACCTA 
CATGAGCTGG AACAGGTCAA AACCTTGCTG CAGGAAACTC TCAGTGCAGA TAAAGGGACC 
ATGTTGCTGA ATAGCCTTGT AGACTATTTC CTTCAGACAA ACTCATCTCA AGCTGTGGAC 
ATCCTGTCTT CTGTCCGGGA GCCTCATGAT AAGTATCTTT TAGATAAGAT GAATGAATGC 
ATGGGCAAGC AGAGCTGTCG TCTCTCCACC ATCACCCTCC TTGGTCATGT TGTTCGCAAA 
CAGCCACCAT GGATCCACAA GATCGCACGC TTCCCTCTGC TTGTCTCGCT GCTCAAATGC 
CTCAAGACTG ACTCAGATGT AGTAGTTCTC ATAACTGGTG TGCTGGTGCT GATCACTCTA 
ATGCCCATGA TTCCTCAAAC CAACAAACAA CTTCTTTGCG ACTACTTTGA TATCTTTGGA 
CGTCTTGCTG CCTGGAACCA ACGTCATCCA GGCCATGCTC AGGGAGTGTT TGCAGTCCAT 
CTACATGCCA GCGTGTACTC TCTTTTCCAT CGTTTATATG GCATGTATCC TTGTAATTTC 
GTTTCGTACC TGCGTGCCCA TTACAGTATG AAGGAAAATG TTGACACGTT CGAGGAAGTG 
GTGAAGCCCA TGTTGGAACA TGTTCGGATA CATCCTGAGC TCATCACTGG AACCAAAGAC 
TACGAGGTTG ATCCAATTAG ATGGAAGAGA TTTGAGCCAC ATGATATTGT GATTGAATGT 
GCCAAAATTT CACTGGATTC TAAAGAGGCG TCATCTGAAG AGGGCTACTC CTCTTTGGCG 
GATCACATAC AGCGACGCGC TGTACACCAT AGCCAATCAT GTTCAGAGCT CAGTTTCCAT 
GAAATCTCAT CAGTCACACC AATAGGTGCT ATTTGTCAGT CTCTCTCTAA ATCACAACCT 
TATCTCACTG TAACCCAAGA CTCAGGTTCC AGCGCACAGA CCCTCAACAA TCAAAGTGAT 
GAGGTCAAAT CCTCAGGGAT GAAGGAGGAG CTGTGGAGCC CTTCCTCCAT ATGTGGGATG 
AGCACCCCTC CCAGTTCTAG AGGAATGTCC CCAACCACAG TTTCTGACAT CTCACTTAGT 
GCCTCACACC TCTCAGGACG GATCCCCAGC ACCCCAGGGG ATGGAAAGTG GACTCTATCT 
CCCACCACAC CCTCGGGGTC ATCCCCTCTC CCATCGCTCT CAGAGGAAAT AGGGCAGGCT 
GCCCAAAACA CCAGCAGTAC CCAGGCTAAG ACGTGCAAAG AGGTGAAGAA AGTCACATCC 
AGAAGTATTA ATAACAGCAG TGAGAATGGA GGTGATGCCA AGACTCCAGT GTCGTTGGCA 
CAGCTCTCTG ACATTGTCAA AAGGACAGAG GGGGAGCGAG AGGATGATAC AATCAATGAG 
GAGATTTTGA GTCTAACCCA TGGGAAACTA GACTTTGGAT CTAGGCCTGG TCTGGAATCC 
TCATTTTCTG GCTCCTTAGA CACCCTTCTC TCATCTCATC CTAATCATGA GCAGAATTTG 
GTATCCACAC CAGACAGGGT TGAATGCTCG GCTAAAGCAG GAGAGCAGCA GCTTCCTAGG 
TCTCTCTGGC CGTTCACCCC CATTGAAAAT GGGAAGCACA GTAAATGTTC TCTGTCTGAT 
CTCACAAAAG AATCCATGCC TTATGAGCCA CTGTTTGACC TCGCCCTGCC GAAGGTGGCA 
TCTCTGTTTT TGGAGAGGAA GACTGCTGAG GCCTTTCGGA GGGTTGAAGG AGAAAGAGAT 
CACCAAGATG AGATGGAAGG AGAGGATCTT TCTGTCACCT CCCCTCTGGA GGTGTTGGAC 
CGGGTTATCC AGCAGGGTCA CGACACTCAT GGGAAAGTGC TGAAGAGAAC TTTCTCTTCT 
CCAAGCCAAT CATCAGACCC ACACTTGAAA GGTAAACAGC ATAGCAATAA AGGAAAAGGT 
CTTGTGCCTG CAGTGTCGGA TGAGCTAGAC ATGTTACGCA GTCAATTGCT GCTGCTGCAT 
AACCAGTTGC TTTATGAGAG ACATAAGAGA GAGCAACATG CGCTTCGTAA CCGTCGTCTG 
TTTGGCCGCA TCGTCAACAC TACTGCACTG GAAGAGCAAA ATAACTCCAT GAAAACGCAG 
CTCAAACTTC AGGAAGTTGA GATCCAGGCT GTAAAGGTAA GCCTGAAAGA GGAGCAGCGG 
CGCTCTGTGC ACATACAGGA AGACAGAGAG GCTGTCGTGA ACCAGTTACA GATTCAAATT 
CAGCAGTTAC AGAAGGAGCG GAATGACTAC TACTCAAAGA TGCAGGTGCT AAAGAGCGAG 
TTGCAGGAGA ATCAGACAAA GGCTGGCAAA ATGGAGGCAG AACTGCAGAA AGCCAATAAC 
AAAGTGTGTA ATATGGGTCA CATGCTCAGC CAGCTATCTA TCAAGCTGAA CAATAGTGAA 
AACATGGAGC AGCAGATGGC TTTCCTGAAT AAGCAGCTGC TGCTTTTGGG TGAAGCCAAC 
AAGTTATACA TGGAAGAAAT TGACCGCTTG GGCCCTGAGG CGCACAAGGA GCTGAATATG 
CTGCAAGCAT CTTCAGTGCG GGAGGGTGAG CGTTTGAGGC AGAGCTCTCT TCAGCAGAGT 
CAGAGACTTG AGGCTGCACA GCAGCGTATC ACAGACCTGG AGAACCAACT CACCAAGAAA 
GAGCAAGTCA TCCGGGAGCA AAAGAAACTG CTGGACAATG TCAAACGACA GGCCACGGAG 
CAGCTGCAGG CTTCAGAAAA CAGGTATTTG GCTCAGAAGA ACATCACTCA GGCATTACAA 
ACTGAGCTGC TTGAGCTCTA TAGCAAGATA GAGCTCAAAA ACCTGAAAAC TAATGCACCA 
GCAGAGTCCA TTTCTGATCC CAGCAGTCCC ACCAACCAGA GGCCAAATGG CAACAGTGCA 
GGCCCATCTG CTCCATCTCT ACCGGCTGAC CTGATGAAAA GAGAGGAAGG GGCGCATGTG 
TGTGTCAATG GTGAAATATC TGCAGCGTCT CCTCAAACAC CCACAGCACT CATTAATGGC 
AGTCAGGAAG AAGATCTTTC CGCCCTTAGT CATTCATTTC CCTCCCTCCC CTGCCAACCC 
TTTGCAGTTG GCTCTTACCC CAGCACCAGA AGCTTTTTGG GTAAGAAAGC CAGAGAAATG 
TTCCGCAATA AGAGTGAGAG CCACTATGAT GGCGACTCGC CCACCCTCAC CTGCCTCTCA 
AAGGATCTTA AAGTTGAGCC AATCACAGAC CCCACAGAGA GTTTGGAGTC CGAGGAAGCT 
GCTGACCAGT TTGACCTGCA GCCAATGGAT AGACCCCGGG CATATAATGT TCAGAGTAGG 
AGGCATCAGG ATCTGCGGAT CATGGACTAT AATGAGACCC ATCATGTGCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171853.1
CDS	187..3600
Translation	MAKEQPSVFDLIPLLGSTDLHELEQVKTLLQETLSADKGTMLLNSLVDYFLQTNSSQAVDILSSVREPHDKYLLDKMNECMGKQSCRLSTITLLGHVVRKQPPWIHKIARFPLLVSLLKCLKTDSDVVVLITGVLVLITLMPMIPQTNKQLLCDYFDIFGRLAAWNQRHPGHAQGVFAVHLHASVYSLFHRLYGMYPCNFVSYLRAHYSMKENVDTFEEVVKPMLEHVRIHPELITGTKDYEVDPIRWKRFEPHDIVIECAKISLDSKEASSEEGYSSLADHIQRRAVHHSQSCSELSFHEISSVTPIGAICQSLSKSQPYLTVTQDSGSSAQTLNNQSDEVKSSGMKEELWSPSSICGMSTPPSSRGMSPTTVSDISLSASHLSGRIPSTPGDGKWTLSPTTPSGSSPLPSLSEEIGQAAQNTSSTQAKTCKEVKKVTSRSINNSSENGGDAKTPVSLAQLSDIVKRTEGEREDDTINEEILSLTHGKLDFGSRPGLESSFSGSLDTLLSSHPNHEQNLVSTPDRVECSAKAGEQQLPRSLWPFTPIENGKHSKCSLSDLTKESMPYEPLFDLALPKVASLFLERKTAEAFRRVEGERDHQDEMEGEDLSVTSPLEVLDRVIQQGHDTHGKVLKRTFSSPSQSSDPHLKGKQHSNKGKGLVPAVSDELDMLRSQLLLLHNQLLYERHKREQHALRNRRLFGRIVNTTALEEQNNSMKTQLKLQEVEIQAVKVSLKEEQRRSVHIQEDREAVVNQLQIQIQQLQKERNDYYSKMQVLKSELQENQTKAGKMEAELQKANNKVCNMGHMLSQLSIKLNNSENMEQQMAFLNKQLLLLGEANKLYMEEIDRLGPEAHKELNMLQASSVREGERLRQSSLQQSQRLEAAQQRITDLENQLTKKEQVIREQKKLLDNVKRQATEQLQASENRYLAQKNITQALQTELLELYSKIELKNLKTNAPAESISDPSSPTNQRPNGNSAGPSAPSLPADLMKREEGAHVCVNGEISAASPQTPTALINGSQEEDLSALSHSFPSLPCQPFAVGSYPSTRSFLGKKAREMFRNKSESHYDGDSPTLTCLSKDLKVEPITDPTESLESEEAADQFDLQPMDRPRAYNVQSRRHQDLRIMDYNETHHVH

Target ORF information:

RefSeq Version	XM_005171796.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tuberous sclerosis 1a (tsc1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171796.3

ORF Insert Sequence:

ATGGCCAAAG AGCAGCCAAG TGTGTTTGAC CTCATCCCTC TTCTGGGGTC CACTGACCTA 
CATGAGCTGG AACAGGTCAA AACCTTGCTG CAGGAAACTC TCAGTGCAGA TAAAGGGACC 
ATGTTGCTGA ATAGCCTTGT AGACTATTTC CTTCAGACAA ACTCATCTCA AGCTGTGGAC 
ATCCTGTCTT CTGTCCGGGA GCCTCATGAT AAGTATCTTT TAGATAAGAT GAATGAATGC 
ATGGGCAAGC AGAGCTGTCG TCTCTCCACC ATCACCCTCC TTGGTCATGT TGTTCGCAAA 
CAGCCACCAT GGATCCACAA GATCGCACGC TTCCCTCTGC TTGTCTCGCT GCTCAAATGC 
CTCAAGACTG ACTCAGATGT AGTAGTTCTC ATAACTGGTG TGCTGGTGCT GATCACTCTA 
ATGCCCATGA TTCCTCAAAC CAACAAACAA CTTCTTTGCG ACTACTTTGA TATCTTTGGA 
CGTCTTGCTG CCTGGAACCA ACGTCATCCA GGCCATGCTC AGGGAGTGTT TGCAGTCCAT 
CTACATGCCA GCGTGTACTC TCTTTTCCAT CGTTTATATG GCATGTATCC TTGTAATTTC 
GTTTCGTACC TGCGTGCCCA TTACAGTATG AAGGAAAATG TTGACACGTT CGAGGAAGTG 
GTGAAGCCCA TGTTGGAACA TGTTCGGATA CATCCTGAGC TCATCACTGG AACCAAAGAC 
TACGAGGTTG ATCCAATTAG ATGGAAGAGA TTTGAGCCAC ATGATATTGT GATTGAATGT 
GCCAAAATTT CACTGGATTC TAAAGAGGCG TCATCTGAAG AGGGCTACTC CTCTTTGGCG 
GATCACATAC AGCGACGCGC TGTACACCAT AGCCAATCAT GTTCAGAGCT CAGTTTCCAT 
GAAATCTCAT CAGTCACACC AATAGGTGCT ATTTGTCAGT CTCTCTCTAA ATCACAACCT 
TATCTCACTG TAACCCAAGA CTCAGGTTCC AGCGCACAGA CCCTCAACAA TCAAAGTGAT 
GAGGTCAAAT CCTCAGGGAT GAAGGAGGAG CTGTGGAGCC CTTCCTCCAT ATGTGGGATG 
AGCACCCCTC CCAGTTCTAG AGGAATGTCC CCAACCACAG TTTCTGACAT CTCACTTAGT 
GCCTCACACC TCTCAGGACG GATCCCCAGC ACCCCAGGGG ATGGAAAGTG GACTCTATCT 
CCCACCACAC CCTCGGGGTC ATCCCCTCTC CCATCGCTCT CAGAGGAAAT AGGGCAGGCT 
GCCCAAAACA CCAGCAGTAC CCAGGCTAAG ACGTGCAAAG AGGTGAAGAA AGTCACATCC 
AGAAGTATTA ATAACAGCAG TGAGAATGGA GGTGATGCCA AGACTCCAGT GTCGTTGGCA 
CAGCTCTCTG ACATTGTCAA AAGGACAGAG GGGGAGCGAG AGGATGATAC AATCAATGAG 
GAGATTTTGA GTCTAACCCA TGGGAAACTA GACTTTGGAT CTAGGCCTGG TCTGGAATCC 
TCATTTTCTG GCTCCTTAGA CACCCTTCTC TCATCTCATC CTAATCATGA GCAGAATTTG 
GTATCCACAC CAGACAGGGT TGAATGCTCG GCTAAAGCAG GAGAGCAGCA GCTTCCTAGG 
TCTCTCTGGC CGTTCACCCC CATTGAAAAT GGGAAGCACA GTAAATGTTC TCTGTCTGAT 
CTCACAAAAG AATCCATGCC TTATGAGCCA CTGTTTGACC TCGCCCTGCC GAAGGTGGCA 
TCTCTGTTTT TGGAGAGGAA GACTGCTGAG GCCTTTCGGA GGGTTGAAGG AGAAAGAGAT 
CACCAAGATG AGATGGAAGG AGAGGATCTT TCTGTCACCT CCCCTCTGGA GGTGTTGGAC 
CGGGTTATCC AGCAGGGTCA CGACACTCAT GGGAAAGTGC TGAAGAGAAC TTTCTCTTCT 
CCAAGCCAAT CATCAGACCC ACACTTGAAA GGTAAACAGC ATAGCAATAA AGGAAAAGGT 
CTTGTGCCTG CAGTGTCGGA TGAGCTAGAC ATGTTACGCA GTCAATTGCT GCTGCTGCAT 
AACCAGTTGC TTTATGAGAG ACATAAGAGA GAGCAACATG CGCTTCGTAA CCGTCGTCTG 
TTTGGCCGCA TCGTCAACAC TACTGCACTG GAAGAGCAAA ATAACTCCAT GAAAACGCAG 
CTCAAACTTC AGGAAGTTGA GATCCAGGCT GTAAAGGTAA GCCTGAAAGA GGAGCAGCGG 
CGCTCTGTGC ACATACAGGA AGACAGAGAG GCTGTCGTGA ACCAGTTACA GATTCAAATT 
CAGCAGTTAC AGAAGGAGCG GAATGACTAC TACTCAAAGA TGCAGGTGCT AAAGAGCGAG 
TTGCAGGAGA ATCAGACAAA GGCTGGCAAA ATGGAGGCAG AACTGCAGAA AGCCAATAAC 
AAAGTGTGTA ATATGGGTCA CATGCTCAGC CAGCTATCTA TCAAGCTGAA CAATAGTGAA 
AACATGGAGC AGCAGATGGC TTTCCTGAAT AAGCAGCTGC TGCTTTTGGG TGAAGCCAAC 
AAGTTATACA TGGAAGAAAT TGACCGCTTG GGCCCTGAGG CGCACAAGGA GCTGAATATG 
CTGCAAGCAT CTTCAGTGCG GGAGGGTGAG CGTTTGAGGC AGAGCTCTCT TCAGCAGAGT 
CAGAGACTTG AGGCTGCACA GCAGCGTATC ACAGACCTGG AGAACCAACT CACCAAGAAA 
GAGCAAGTCA TCCGGGAGCA AAAGAAACTG CTGGACAATG TCAAACGACA GGCCACGGAG 
CAGCTGCAGG CTTCAGAAAA CAGGTATTTG GCTCAGAAGA ACATCACTCA GGCATTACAA 
ACTGAGCTGC TTGAGCTCTA TAGCAAGATA GAGCTCAAAA ACCTGAAAAC TAATGCACCA 
GCAGAGTCCA TTTCTGATCC CAGCAGTCCC ACCAACCAGA GGCCAAATGG CAACAGTGCA 
GGCCCATCTG CTCCATCTCT ACCGGCTGAC CTGATGAAAA GAGAGGAAGG GGCGCATGTG 
TGTGTCAATG GTGAAATATC TGCAGCGTCT CCTCAAACAC CCACAGCACT CATTAATGGC 
AGTCAGGAAG AAGATCTTTC CGCCCTTAGT CATTCATTTC CCTCCCTCCC CTGCCAACCC 
TTTGCAGTTG GCTCTTACCC CAGCACCAGA AGCTTTTTGG GTAAGAAAGC CAGAGAAATG 
TTCCGCAATA AGAGTGAGAG CCACTATGAT GGCGACTCGC CCACCCTCAC CTGCCTCTCA 
AAGGATCTTA AAGTTGAGCC AATCACAGAC CCCACAGAGA GTTTGGAGTC CGAGGAAGCT 
GCTGACCAGT TTGACCTGCA GCCAATGGAT AGACCCCGGG CATATAATGT TCAGAGTAGG 
AGGCATCAGG ATCTGCGGAT CATGGACTAT AATGAGACCC ATCATGTGCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1A ( XM_005171796.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005171853.1 Danio rerio tuberous sclerosis 1a (tsc1a), transcript variant X2, mRNA.

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CloneID	ODa10423
Clone ID Related Accession (Same CDS sequence)	XM_005171795.4 , XM_005171796.3 , XM_005171796.4 , XM_005171795.3
Accession Version	XM_005171796.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171796.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG AGCAGCCAAG TGTGTTTGAC CTCATCCCTC TTCTGGGGTC CACTGACCTA 
CATGAGCTGG AACAGGTCAA AACCTTGCTG CAGGAAACTC TCAGTGCAGA TAAAGGGACC 
ATGTTGCTGA ATAGCCTTGT AGACTATTTC CTTCAGACAA ACTCATCTCA AGCTGTGGAC 
ATCCTGTCTT CTGTCCGGGA GCCTCATGAT AAGTATCTTT TAGATAAGAT GAATGAATGC 
ATGGGCAAGC AGAGCTGTCG TCTCTCCACC ATCACCCTCC TTGGTCATGT TGTTCGCAAA 
CAGCCACCAT GGATCCACAA GATCGCACGC TTCCCTCTGC TTGTCTCGCT GCTCAAATGC 
CTCAAGACTG ACTCAGATGT AGTAGTTCTC ATAACTGGTG TGCTGGTGCT GATCACTCTA 
ATGCCCATGA TTCCTCAAAC CAACAAACAA CTTCTTTGCG ACTACTTTGA TATCTTTGGA 
CGTCTTGCTG CCTGGAACCA ACGTCATCCA GGCCATGCTC AGGGAGTGTT TGCAGTCCAT 
CTACATGCCA GCGTGTACTC TCTTTTCCAT CGTTTATATG GCATGTATCC TTGTAATTTC 
GTTTCGTACC TGCGTGCCCA TTACAGTATG AAGGAAAATG TTGACACGTT CGAGGAAGTG 
GTGAAGCCCA TGTTGGAACA TGTTCGGATA CATCCTGAGC TCATCACTGG AACCAAAGAC 
TACGAGGTTG ATCCAATTAG ATGGAAGAGA TTTGAGCCAC ATGATATTGT GATTGAATGT 
GCCAAAATTT CACTGGATTC TAAAGAGGCG TCATCTGAAG AGGGCTACTC CTCTTTGGCG 
GATCACATAC AGCGACGCGC TGTACACCAT AGCCAATCAT GTTCAGAGCT CAGTTTCCAT 
GAAATCTCAT CAGTCACACC AATAGGTGCT ATTTGTCAGT CTCTCTCTAA ATCACAACCT 
TATCTCACTG TAACCCAAGA CTCAGGTTCC AGCGCACAGA CCCTCAACAA TCAAAGTGAT 
GAGGTCAAAT CCTCAGGGAT GAAGGAGGAG CTGTGGAGCC CTTCCTCCAT ATGTGGGATG 
AGCACCCCTC CCAGTTCTAG AGGAATGTCC CCAACCACAG TTTCTGACAT CTCACTTAGT 
GCCTCACACC TCTCAGGACG GATCCCCAGC ACCCCAGGGG ATGGAAAGTG GACTCTATCT 
CCCACCACAC CCTCGGGGTC ATCCCCTCTC CCATCGCTCT CAGAGGAAAT AGGGCAGGCT 
GCCCAAAACA CCAGCAGTAC CCAGGCTAAG ACGTGCAAAG AGGTGAAGAA AGTCACATCC 
AGAAGTATTA ATAACAGCAG TGAGAATGGA GGTGATGCCA AGACTCCAGT GTCGTTGGCA 
CAGCTCTCTG ACATTGTCAA AAGGACAGAG GGGGAGCGAG AGGATGATAC AATCAATGAG 
GAGATTTTGA GTCTAACCCA TGGGAAACTA GACTTTGGAT CTAGGCCTGG TCTGGAATCC 
TCATTTTCTG GCTCCTTAGA CACCCTTCTC TCATCTCATC CTAATCATGA GCAGAATTTG 
GTATCCACAC CAGACAGGGT TGAATGCTCG GCTAAAGCAG GAGAGCAGCA GCTTCCTAGG 
TCTCTCTGGC CGTTCACCCC CATTGAAAAT GGGAAGCACA GTAAATGTTC TCTGTCTGAT 
CTCACAAAAG AATCCATGCC TTATGAGCCA CTGTTTGACC TCGCCCTGCC GAAGGTGGCA 
TCTCTGTTTT TGGAGAGGAA GACTGCTGAG GCCTTTCGGA GGGTTGAAGG AGAAAGAGAT 
CACCAAGATG AGATGGAAGG AGAGGATCTT TCTGTCACCT CCCCTCTGGA GGTGTTGGAC 
CGGGTTATCC AGCAGGGTCA CGACACTCAT GGGAAAGTGC TGAAGAGAAC TTTCTCTTCT 
CCAAGCCAAT CATCAGACCC ACACTTGAAA GGTAAACAGC ATAGCAATAA AGGAAAAGGT 
CTTGTGCCTG CAGTGTCGGA TGAGCTAGAC ATGTTACGCA GTCAATTGCT GCTGCTGCAT 
AACCAGTTGC TTTATGAGAG ACATAAGAGA GAGCAACATG CGCTTCGTAA CCGTCGTCTG 
TTTGGCCGCA TCGTCAACAC TACTGCACTG GAAGAGCAAA ATAACTCCAT GAAAACGCAG 
CTCAAACTTC AGGAAGTTGA GATCCAGGCT GTAAAGGTAA GCCTGAAAGA GGAGCAGCGG 
CGCTCTGTGC ACATACAGGA AGACAGAGAG GCTGTCGTGA ACCAGTTACA GATTCAAATT 
CAGCAGTTAC AGAAGGAGCG GAATGACTAC TACTCAAAGA TGCAGGTGCT AAAGAGCGAG 
TTGCAGGAGA ATCAGACAAA GGCTGGCAAA ATGGAGGCAG AACTGCAGAA AGCCAATAAC 
AAAGTGTGTA ATATGGGTCA CATGCTCAGC CAGCTATCTA TCAAGCTGAA CAATAGTGAA 
AACATGGAGC AGCAGATGGC TTTCCTGAAT AAGCAGCTGC TGCTTTTGGG TGAAGCCAAC 
AAGTTATACA TGGAAGAAAT TGACCGCTTG GGCCCTGAGG CGCACAAGGA GCTGAATATG 
CTGCAAGCAT CTTCAGTGCG GGAGGGTGAG CGTTTGAGGC AGAGCTCTCT TCAGCAGAGT 
CAGAGACTTG AGGCTGCACA GCAGCGTATC ACAGACCTGG AGAACCAACT CACCAAGAAA 
GAGCAAGTCA TCCGGGAGCA AAAGAAACTG CTGGACAATG TCAAACGACA GGCCACGGAG 
CAGCTGCAGG CTTCAGAAAA CAGGTATTTG GCTCAGAAGA ACATCACTCA GGCATTACAA 
ACTGAGCTGC TTGAGCTCTA TAGCAAGATA GAGCTCAAAA ACCTGAAAAC TAATGCACCA 
GCAGAGTCCA TTTCTGATCC CAGCAGTCCC ACCAACCAGA GGCCAAATGG CAACAGTGCA 
GGCCCATCTG CTCCATCTCT ACCGGCTGAC CTGATGAAAA GAGAGGAAGG GGCGCATGTG 
TGTGTCAATG GTGAAATATC TGCAGCGTCT CCTCAAACAC CCACAGCACT CATTAATGGC 
AGTCAGGAAG AAGATCTTTC CGCCCTTAGT CATTCATTTC CCTCCCTCCC CTGCCAACCC 
TTTGCAGTTG GCTCTTACCC CAGCACCAGA AGCTTTTTGG GTAAGAAAGC CAGAGAAATG 
TTCCGCAATA AGAGTGAGAG CCACTATGAT GGCGACTCGC CCACCCTCAC CTGCCTCTCA 
AAGGATCTTA AAGTTGAGCC AATCACAGAC CCCACAGAGA GTTTGGAGTC CGAGGAAGCT 
GCTGACCAGT TTGACCTGCA GCCAATGGAT AGACCCCGGG CATATAATGT TCAGAGTAGG 
AGGCATCAGG ATCTGCGGAT CATGGACTAT AATGAGACCC ATCATGTGCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171853.1
CDS	232..3645
Translation	MAKEQPSVFDLIPLLGSTDLHELEQVKTLLQETLSADKGTMLLNSLVDYFLQTNSSQAVDILSSVREPHDKYLLDKMNECMGKQSCRLSTITLLGHVVRKQPPWIHKIARFPLLVSLLKCLKTDSDVVVLITGVLVLITLMPMIPQTNKQLLCDYFDIFGRLAAWNQRHPGHAQGVFAVHLHASVYSLFHRLYGMYPCNFVSYLRAHYSMKENVDTFEEVVKPMLEHVRIHPELITGTKDYEVDPIRWKRFEPHDIVIECAKISLDSKEASSEEGYSSLADHIQRRAVHHSQSCSELSFHEISSVTPIGAICQSLSKSQPYLTVTQDSGSSAQTLNNQSDEVKSSGMKEELWSPSSICGMSTPPSSRGMSPTTVSDISLSASHLSGRIPSTPGDGKWTLSPTTPSGSSPLPSLSEEIGQAAQNTSSTQAKTCKEVKKVTSRSINNSSENGGDAKTPVSLAQLSDIVKRTEGEREDDTINEEILSLTHGKLDFGSRPGLESSFSGSLDTLLSSHPNHEQNLVSTPDRVECSAKAGEQQLPRSLWPFTPIENGKHSKCSLSDLTKESMPYEPLFDLALPKVASLFLERKTAEAFRRVEGERDHQDEMEGEDLSVTSPLEVLDRVIQQGHDTHGKVLKRTFSSPSQSSDPHLKGKQHSNKGKGLVPAVSDELDMLRSQLLLLHNQLLYERHKREQHALRNRRLFGRIVNTTALEEQNNSMKTQLKLQEVEIQAVKVSLKEEQRRSVHIQEDREAVVNQLQIQIQQLQKERNDYYSKMQVLKSELQENQTKAGKMEAELQKANNKVCNMGHMLSQLSIKLNNSENMEQQMAFLNKQLLLLGEANKLYMEEIDRLGPEAHKELNMLQASSVREGERLRQSSLQQSQRLEAAQQRITDLENQLTKKEQVIREQKKLLDNVKRQATEQLQASENRYLAQKNITQALQTELLELYSKIELKNLKTNAPAESISDPSSPTNQRPNGNSAGPSAPSLPADLMKREEGAHVCVNGEISAASPQTPTALINGSQEEDLSALSHSFPSLPCQPFAVGSYPSTRSFLGKKAREMFRNKSESHYDGDSPTLTCLSKDLKVEPITDPTESLESEEAADQFDLQPMDRPRAYNVQSRRHQDLRIMDYNETHHVH

Target ORF information:

RefSeq Version	XM_005171796.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tuberous sclerosis 1a (tsc1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171796.4

ORF Insert Sequence:

ATGGCCAAAG AGCAGCCAAG TGTGTTTGAC CTCATCCCTC TTCTGGGGTC CACTGACCTA 
CATGAGCTGG AACAGGTCAA AACCTTGCTG CAGGAAACTC TCAGTGCAGA TAAAGGGACC 
ATGTTGCTGA ATAGCCTTGT AGACTATTTC CTTCAGACAA ACTCATCTCA AGCTGTGGAC 
ATCCTGTCTT CTGTCCGGGA GCCTCATGAT AAGTATCTTT TAGATAAGAT GAATGAATGC 
ATGGGCAAGC AGAGCTGTCG TCTCTCCACC ATCACCCTCC TTGGTCATGT TGTTCGCAAA 
CAGCCACCAT GGATCCACAA GATCGCACGC TTCCCTCTGC TTGTCTCGCT GCTCAAATGC 
CTCAAGACTG ACTCAGATGT AGTAGTTCTC ATAACTGGTG TGCTGGTGCT GATCACTCTA 
ATGCCCATGA TTCCTCAAAC CAACAAACAA CTTCTTTGCG ACTACTTTGA TATCTTTGGA 
CGTCTTGCTG CCTGGAACCA ACGTCATCCA GGCCATGCTC AGGGAGTGTT TGCAGTCCAT 
CTACATGCCA GCGTGTACTC TCTTTTCCAT CGTTTATATG GCATGTATCC TTGTAATTTC 
GTTTCGTACC TGCGTGCCCA TTACAGTATG AAGGAAAATG TTGACACGTT CGAGGAAGTG 
GTGAAGCCCA TGTTGGAACA TGTTCGGATA CATCCTGAGC TCATCACTGG AACCAAAGAC 
TACGAGGTTG ATCCAATTAG ATGGAAGAGA TTTGAGCCAC ATGATATTGT GATTGAATGT 
GCCAAAATTT CACTGGATTC TAAAGAGGCG TCATCTGAAG AGGGCTACTC CTCTTTGGCG 
GATCACATAC AGCGACGCGC TGTACACCAT AGCCAATCAT GTTCAGAGCT CAGTTTCCAT 
GAAATCTCAT CAGTCACACC AATAGGTGCT ATTTGTCAGT CTCTCTCTAA ATCACAACCT 
TATCTCACTG TAACCCAAGA CTCAGGTTCC AGCGCACAGA CCCTCAACAA TCAAAGTGAT 
GAGGTCAAAT CCTCAGGGAT GAAGGAGGAG CTGTGGAGCC CTTCCTCCAT ATGTGGGATG 
AGCACCCCTC CCAGTTCTAG AGGAATGTCC CCAACCACAG TTTCTGACAT CTCACTTAGT 
GCCTCACACC TCTCAGGACG GATCCCCAGC ACCCCAGGGG ATGGAAAGTG GACTCTATCT 
CCCACCACAC CCTCGGGGTC ATCCCCTCTC CCATCGCTCT CAGAGGAAAT AGGGCAGGCT 
GCCCAAAACA CCAGCAGTAC CCAGGCTAAG ACGTGCAAAG AGGTGAAGAA AGTCACATCC 
AGAAGTATTA ATAACAGCAG TGAGAATGGA GGTGATGCCA AGACTCCAGT GTCGTTGGCA 
CAGCTCTCTG ACATTGTCAA AAGGACAGAG GGGGAGCGAG AGGATGATAC AATCAATGAG 
GAGATTTTGA GTCTAACCCA TGGGAAACTA GACTTTGGAT CTAGGCCTGG TCTGGAATCC 
TCATTTTCTG GCTCCTTAGA CACCCTTCTC TCATCTCATC CTAATCATGA GCAGAATTTG 
GTATCCACAC CAGACAGGGT TGAATGCTCG GCTAAAGCAG GAGAGCAGCA GCTTCCTAGG 
TCTCTCTGGC CGTTCACCCC CATTGAAAAT GGGAAGCACA GTAAATGTTC TCTGTCTGAT 
CTCACAAAAG AATCCATGCC TTATGAGCCA CTGTTTGACC TCGCCCTGCC GAAGGTGGCA 
TCTCTGTTTT TGGAGAGGAA GACTGCTGAG GCCTTTCGGA GGGTTGAAGG AGAAAGAGAT 
CACCAAGATG AGATGGAAGG AGAGGATCTT TCTGTCACCT CCCCTCTGGA GGTGTTGGAC 
CGGGTTATCC AGCAGGGTCA CGACACTCAT GGGAAAGTGC TGAAGAGAAC TTTCTCTTCT 
CCAAGCCAAT CATCAGACCC ACACTTGAAA GGTAAACAGC ATAGCAATAA AGGAAAAGGT 
CTTGTGCCTG CAGTGTCGGA TGAGCTAGAC ATGTTACGCA GTCAATTGCT GCTGCTGCAT 
AACCAGTTGC TTTATGAGAG ACATAAGAGA GAGCAACATG CGCTTCGTAA CCGTCGTCTG 
TTTGGCCGCA TCGTCAACAC TACTGCACTG GAAGAGCAAA ATAACTCCAT GAAAACGCAG 
CTCAAACTTC AGGAAGTTGA GATCCAGGCT GTAAAGGTAA GCCTGAAAGA GGAGCAGCGG 
CGCTCTGTGC ACATACAGGA AGACAGAGAG GCTGTCGTGA ACCAGTTACA GATTCAAATT 
CAGCAGTTAC AGAAGGAGCG GAATGACTAC TACTCAAAGA TGCAGGTGCT AAAGAGCGAG 
TTGCAGGAGA ATCAGACAAA GGCTGGCAAA ATGGAGGCAG AACTGCAGAA AGCCAATAAC 
AAAGTGTGTA ATATGGGTCA CATGCTCAGC CAGCTATCTA TCAAGCTGAA CAATAGTGAA 
AACATGGAGC AGCAGATGGC TTTCCTGAAT AAGCAGCTGC TGCTTTTGGG TGAAGCCAAC 
AAGTTATACA TGGAAGAAAT TGACCGCTTG GGCCCTGAGG CGCACAAGGA GCTGAATATG 
CTGCAAGCAT CTTCAGTGCG GGAGGGTGAG CGTTTGAGGC AGAGCTCTCT TCAGCAGAGT 
CAGAGACTTG AGGCTGCACA GCAGCGTATC ACAGACCTGG AGAACCAACT CACCAAGAAA 
GAGCAAGTCA TCCGGGAGCA AAAGAAACTG CTGGACAATG TCAAACGACA GGCCACGGAG 
CAGCTGCAGG CTTCAGAAAA CAGGTATTTG GCTCAGAAGA ACATCACTCA GGCATTACAA 
ACTGAGCTGC TTGAGCTCTA TAGCAAGATA GAGCTCAAAA ACCTGAAAAC TAATGCACCA 
GCAGAGTCCA TTTCTGATCC CAGCAGTCCC ACCAACCAGA GGCCAAATGG CAACAGTGCA 
GGCCCATCTG CTCCATCTCT ACCGGCTGAC CTGATGAAAA GAGAGGAAGG GGCGCATGTG 
TGTGTCAATG GTGAAATATC TGCAGCGTCT CCTCAAACAC CCACAGCACT CATTAATGGC 
AGTCAGGAAG AAGATCTTTC CGCCCTTAGT CATTCATTTC CCTCCCTCCC CTGCCAACCC 
TTTGCAGTTG GCTCTTACCC CAGCACCAGA AGCTTTTTGG GTAAGAAAGC CAGAGAAATG 
TTCCGCAATA AGAGTGAGAG CCACTATGAT GGCGACTCGC CCACCCTCAC CTGCCTCTCA 
AAGGATCTTA AAGTTGAGCC AATCACAGAC CCCACAGAGA GTTTGGAGTC CGAGGAAGCT 
GCTGACCAGT TTGACCTGCA GCCAATGGAT AGACCCCGGG CATATAATGT TCAGAGTAGG 
AGGCATCAGG ATCTGCGGAT CATGGACTAT AATGAGACCC ATCATGTGCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1A ( XM_005171795.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005171852.1 Danio rerio tuberous sclerosis 1a (tsc1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10423
Clone ID Related Accession (Same CDS sequence)	XM_005171795.4 , XM_005171796.3 , XM_005171796.4 , XM_005171795.3
Accession Version	XM_005171795.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	hamartin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171795.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG AGCAGCCAAG TGTGTTTGAC CTCATCCCTC TTCTGGGGTC CACTGACCTA 
CATGAGCTGG AACAGGTCAA AACCTTGCTG CAGGAAACTC TCAGTGCAGA TAAAGGGACC 
ATGTTGCTGA ATAGCCTTGT AGACTATTTC CTTCAGACAA ACTCATCTCA AGCTGTGGAC 
ATCCTGTCTT CTGTCCGGGA GCCTCATGAT AAGTATCTTT TAGATAAGAT GAATGAATGC 
ATGGGCAAGC AGAGCTGTCG TCTCTCCACC ATCACCCTCC TTGGTCATGT TGTTCGCAAA 
CAGCCACCAT GGATCCACAA GATCGCACGC TTCCCTCTGC TTGTCTCGCT GCTCAAATGC 
CTCAAGACTG ACTCAGATGT AGTAGTTCTC ATAACTGGTG TGCTGGTGCT GATCACTCTA 
ATGCCCATGA TTCCTCAAAC CAACAAACAA CTTCTTTGCG ACTACTTTGA TATCTTTGGA 
CGTCTTGCTG CCTGGAACCA ACGTCATCCA GGCCATGCTC AGGGAGTGTT TGCAGTCCAT 
CTACATGCCA GCGTGTACTC TCTTTTCCAT CGTTTATATG GCATGTATCC TTGTAATTTC 
GTTTCGTACC TGCGTGCCCA TTACAGTATG AAGGAAAATG TTGACACGTT CGAGGAAGTG 
GTGAAGCCCA TGTTGGAACA TGTTCGGATA CATCCTGAGC TCATCACTGG AACCAAAGAC 
TACGAGGTTG ATCCAATTAG ATGGAAGAGA TTTGAGCCAC ATGATATTGT GATTGAATGT 
GCCAAAATTT CACTGGATTC TAAAGAGGCG TCATCTGAAG AGGGCTACTC CTCTTTGGCG 
GATCACATAC AGCGACGCGC TGTACACCAT AGCCAATCAT GTTCAGAGCT CAGTTTCCAT 
GAAATCTCAT CAGTCACACC AATAGGTGCT ATTTGTCAGT CTCTCTCTAA ATCACAACCT 
TATCTCACTG TAACCCAAGA CTCAGGTTCC AGCGCACAGA CCCTCAACAA TCAAAGTGAT 
GAGGTCAAAT CCTCAGGGAT GAAGGAGGAG CTGTGGAGCC CTTCCTCCAT ATGTGGGATG 
AGCACCCCTC CCAGTTCTAG AGGAATGTCC CCAACCACAG TTTCTGACAT CTCACTTAGT 
GCCTCACACC TCTCAGGACG GATCCCCAGC ACCCCAGGGG ATGGAAAGTG GACTCTATCT 
CCCACCACAC CCTCGGGGTC ATCCCCTCTC CCATCGCTCT CAGAGGAAAT AGGGCAGGCT 
GCCCAAAACA CCAGCAGTAC CCAGGCTAAG ACGTGCAAAG AGGTGAAGAA AGTCACATCC 
AGAAGTATTA ATAACAGCAG TGAGAATGGA GGTGATGCCA AGACTCCAGT GTCGTTGGCA 
CAGCTCTCTG ACATTGTCAA AAGGACAGAG GGGGAGCGAG AGGATGATAC AATCAATGAG 
GAGATTTTGA GTCTAACCCA TGGGAAACTA GACTTTGGAT CTAGGCCTGG TCTGGAATCC 
TCATTTTCTG GCTCCTTAGA CACCCTTCTC TCATCTCATC CTAATCATGA GCAGAATTTG 
GTATCCACAC CAGACAGGGT TGAATGCTCG GCTAAAGCAG GAGAGCAGCA GCTTCCTAGG 
TCTCTCTGGC CGTTCACCCC CATTGAAAAT GGGAAGCACA GTAAATGTTC TCTGTCTGAT 
CTCACAAAAG AATCCATGCC TTATGAGCCA CTGTTTGACC TCGCCCTGCC GAAGGTGGCA 
TCTCTGTTTT TGGAGAGGAA GACTGCTGAG GCCTTTCGGA GGGTTGAAGG AGAAAGAGAT 
CACCAAGATG AGATGGAAGG AGAGGATCTT TCTGTCACCT CCCCTCTGGA GGTGTTGGAC 
CGGGTTATCC AGCAGGGTCA CGACACTCAT GGGAAAGTGC TGAAGAGAAC TTTCTCTTCT 
CCAAGCCAAT CATCAGACCC ACACTTGAAA GGTAAACAGC ATAGCAATAA AGGAAAAGGT 
CTTGTGCCTG CAGTGTCGGA TGAGCTAGAC ATGTTACGCA GTCAATTGCT GCTGCTGCAT 
AACCAGTTGC TTTATGAGAG ACATAAGAGA GAGCAACATG CGCTTCGTAA CCGTCGTCTG 
TTTGGCCGCA TCGTCAACAC TACTGCACTG GAAGAGCAAA ATAACTCCAT GAAAACGCAG 
CTCAAACTTC AGGAAGTTGA GATCCAGGCT GTAAAGGTAA GCCTGAAAGA GGAGCAGCGG 
CGCTCTGTGC ACATACAGGA AGACAGAGAG GCTGTCGTGA ACCAGTTACA GATTCAAATT 
CAGCAGTTAC AGAAGGAGCG GAATGACTAC TACTCAAAGA TGCAGGTGCT AAAGAGCGAG 
TTGCAGGAGA ATCAGACAAA GGCTGGCAAA ATGGAGGCAG AACTGCAGAA AGCCAATAAC 
AAAGTGTGTA ATATGGGTCA CATGCTCAGC CAGCTATCTA TCAAGCTGAA CAATAGTGAA 
AACATGGAGC AGCAGATGGC TTTCCTGAAT AAGCAGCTGC TGCTTTTGGG TGAAGCCAAC 
AAGTTATACA TGGAAGAAAT TGACCGCTTG GGCCCTGAGG CGCACAAGGA GCTGAATATG 
CTGCAAGCAT CTTCAGTGCG GGAGGGTGAG CGTTTGAGGC AGAGCTCTCT TCAGCAGAGT 
CAGAGACTTG AGGCTGCACA GCAGCGTATC ACAGACCTGG AGAACCAACT CACCAAGAAA 
GAGCAAGTCA TCCGGGAGCA AAAGAAACTG CTGGACAATG TCAAACGACA GGCCACGGAG 
CAGCTGCAGG CTTCAGAAAA CAGGTATTTG GCTCAGAAGA ACATCACTCA GGCATTACAA 
ACTGAGCTGC TTGAGCTCTA TAGCAAGATA GAGCTCAAAA ACCTGAAAAC TAATGCACCA 
GCAGAGTCCA TTTCTGATCC CAGCAGTCCC ACCAACCAGA GGCCAAATGG CAACAGTGCA 
GGCCCATCTG CTCCATCTCT ACCGGCTGAC CTGATGAAAA GAGAGGAAGG GGCGCATGTG 
TGTGTCAATG GTGAAATATC TGCAGCGTCT CCTCAAACAC CCACAGCACT CATTAATGGC 
AGTCAGGAAG AAGATCTTTC CGCCCTTAGT CATTCATTTC CCTCCCTCCC CTGCCAACCC 
TTTGCAGTTG GCTCTTACCC CAGCACCAGA AGCTTTTTGG GTAAGAAAGC CAGAGAAATG 
TTCCGCAATA AGAGTGAGAG CCACTATGAT GGCGACTCGC CCACCCTCAC CTGCCTCTCA 
AAGGATCTTA AAGTTGAGCC AATCACAGAC CCCACAGAGA GTTTGGAGTC CGAGGAAGCT 
GCTGACCAGT TTGACCTGCA GCCAATGGAT AGACCCCGGG CATATAATGT TCAGAGTAGG 
AGGCATCAGG ATCTGCGGAT CATGGACTAT AATGAGACCC ATCATGTGCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171852.1
CDS	217..3630
Translation	MAKEQPSVFDLIPLLGSTDLHELEQVKTLLQETLSADKGTMLLNSLVDYFLQTNSSQAVDILSSVREPHDKYLLDKMNECMGKQSCRLSTITLLGHVVRKQPPWIHKIARFPLLVSLLKCLKTDSDVVVLITGVLVLITLMPMIPQTNKQLLCDYFDIFGRLAAWNQRHPGHAQGVFAVHLHASVYSLFHRLYGMYPCNFVSYLRAHYSMKENVDTFEEVVKPMLEHVRIHPELITGTKDYEVDPIRWKRFEPHDIVIECAKISLDSKEASSEEGYSSLADHIQRRAVHHSQSCSELSFHEISSVTPIGAICQSLSKSQPYLTVTQDSGSSAQTLNNQSDEVKSSGMKEELWSPSSICGMSTPPSSRGMSPTTVSDISLSASHLSGRIPSTPGDGKWTLSPTTPSGSSPLPSLSEEIGQAAQNTSSTQAKTCKEVKKVTSRSINNSSENGGDAKTPVSLAQLSDIVKRTEGEREDDTINEEILSLTHGKLDFGSRPGLESSFSGSLDTLLSSHPNHEQNLVSTPDRVECSAKAGEQQLPRSLWPFTPIENGKHSKCSLSDLTKESMPYEPLFDLALPKVASLFLERKTAEAFRRVEGERDHQDEMEGEDLSVTSPLEVLDRVIQQGHDTHGKVLKRTFSSPSQSSDPHLKGKQHSNKGKGLVPAVSDELDMLRSQLLLLHNQLLYERHKREQHALRNRRLFGRIVNTTALEEQNNSMKTQLKLQEVEIQAVKVSLKEEQRRSVHIQEDREAVVNQLQIQIQQLQKERNDYYSKMQVLKSELQENQTKAGKMEAELQKANNKVCNMGHMLSQLSIKLNNSENMEQQMAFLNKQLLLLGEANKLYMEEIDRLGPEAHKELNMLQASSVREGERLRQSSLQQSQRLEAAQQRITDLENQLTKKEQVIREQKKLLDNVKRQATEQLQASENRYLAQKNITQALQTELLELYSKIELKNLKTNAPAESISDPSSPTNQRPNGNSAGPSAPSLPADLMKREEGAHVCVNGEISAASPQTPTALINGSQEEDLSALSHSFPSLPCQPFAVGSYPSTRSFLGKKAREMFRNKSESHYDGDSPTLTCLSKDLKVEPITDPTESLESEEAADQFDLQPMDRPRAYNVQSRRHQDLRIMDYNETHHVH

Target ORF information:

RefSeq Version	XM_005171795.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tuberous sclerosis 1a (tsc1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171795.3

ORF Insert Sequence:

ATGGCCAAAG AGCAGCCAAG TGTGTTTGAC CTCATCCCTC TTCTGGGGTC CACTGACCTA 
CATGAGCTGG AACAGGTCAA AACCTTGCTG CAGGAAACTC TCAGTGCAGA TAAAGGGACC 
ATGTTGCTGA ATAGCCTTGT AGACTATTTC CTTCAGACAA ACTCATCTCA AGCTGTGGAC 
ATCCTGTCTT CTGTCCGGGA GCCTCATGAT AAGTATCTTT TAGATAAGAT GAATGAATGC 
ATGGGCAAGC AGAGCTGTCG TCTCTCCACC ATCACCCTCC TTGGTCATGT TGTTCGCAAA 
CAGCCACCAT GGATCCACAA GATCGCACGC TTCCCTCTGC TTGTCTCGCT GCTCAAATGC 
CTCAAGACTG ACTCAGATGT AGTAGTTCTC ATAACTGGTG TGCTGGTGCT GATCACTCTA 
ATGCCCATGA TTCCTCAAAC CAACAAACAA CTTCTTTGCG ACTACTTTGA TATCTTTGGA 
CGTCTTGCTG CCTGGAACCA ACGTCATCCA GGCCATGCTC AGGGAGTGTT TGCAGTCCAT 
CTACATGCCA GCGTGTACTC TCTTTTCCAT CGTTTATATG GCATGTATCC TTGTAATTTC 
GTTTCGTACC TGCGTGCCCA TTACAGTATG AAGGAAAATG TTGACACGTT CGAGGAAGTG 
GTGAAGCCCA TGTTGGAACA TGTTCGGATA CATCCTGAGC TCATCACTGG AACCAAAGAC 
TACGAGGTTG ATCCAATTAG ATGGAAGAGA TTTGAGCCAC ATGATATTGT GATTGAATGT 
GCCAAAATTT CACTGGATTC TAAAGAGGCG TCATCTGAAG AGGGCTACTC CTCTTTGGCG 
GATCACATAC AGCGACGCGC TGTACACCAT AGCCAATCAT GTTCAGAGCT CAGTTTCCAT 
GAAATCTCAT CAGTCACACC AATAGGTGCT ATTTGTCAGT CTCTCTCTAA ATCACAACCT 
TATCTCACTG TAACCCAAGA CTCAGGTTCC AGCGCACAGA CCCTCAACAA TCAAAGTGAT 
GAGGTCAAAT CCTCAGGGAT GAAGGAGGAG CTGTGGAGCC CTTCCTCCAT ATGTGGGATG 
AGCACCCCTC CCAGTTCTAG AGGAATGTCC CCAACCACAG TTTCTGACAT CTCACTTAGT 
GCCTCACACC TCTCAGGACG GATCCCCAGC ACCCCAGGGG ATGGAAAGTG GACTCTATCT 
CCCACCACAC CCTCGGGGTC ATCCCCTCTC CCATCGCTCT CAGAGGAAAT AGGGCAGGCT 
GCCCAAAACA CCAGCAGTAC CCAGGCTAAG ACGTGCAAAG AGGTGAAGAA AGTCACATCC 
AGAAGTATTA ATAACAGCAG TGAGAATGGA GGTGATGCCA AGACTCCAGT GTCGTTGGCA 
CAGCTCTCTG ACATTGTCAA AAGGACAGAG GGGGAGCGAG AGGATGATAC AATCAATGAG 
GAGATTTTGA GTCTAACCCA TGGGAAACTA GACTTTGGAT CTAGGCCTGG TCTGGAATCC 
TCATTTTCTG GCTCCTTAGA CACCCTTCTC TCATCTCATC CTAATCATGA GCAGAATTTG 
GTATCCACAC CAGACAGGGT TGAATGCTCG GCTAAAGCAG GAGAGCAGCA GCTTCCTAGG 
TCTCTCTGGC CGTTCACCCC CATTGAAAAT GGGAAGCACA GTAAATGTTC TCTGTCTGAT 
CTCACAAAAG AATCCATGCC TTATGAGCCA CTGTTTGACC TCGCCCTGCC GAAGGTGGCA 
TCTCTGTTTT TGGAGAGGAA GACTGCTGAG GCCTTTCGGA GGGTTGAAGG AGAAAGAGAT 
CACCAAGATG AGATGGAAGG AGAGGATCTT TCTGTCACCT CCCCTCTGGA GGTGTTGGAC 
CGGGTTATCC AGCAGGGTCA CGACACTCAT GGGAAAGTGC TGAAGAGAAC TTTCTCTTCT 
CCAAGCCAAT CATCAGACCC ACACTTGAAA GGTAAACAGC ATAGCAATAA AGGAAAAGGT 
CTTGTGCCTG CAGTGTCGGA TGAGCTAGAC ATGTTACGCA GTCAATTGCT GCTGCTGCAT 
AACCAGTTGC TTTATGAGAG ACATAAGAGA GAGCAACATG CGCTTCGTAA CCGTCGTCTG 
TTTGGCCGCA TCGTCAACAC TACTGCACTG GAAGAGCAAA ATAACTCCAT GAAAACGCAG 
CTCAAACTTC AGGAAGTTGA GATCCAGGCT GTAAAGGTAA GCCTGAAAGA GGAGCAGCGG 
CGCTCTGTGC ACATACAGGA AGACAGAGAG GCTGTCGTGA ACCAGTTACA GATTCAAATT 
CAGCAGTTAC AGAAGGAGCG GAATGACTAC TACTCAAAGA TGCAGGTGCT AAAGAGCGAG 
TTGCAGGAGA ATCAGACAAA GGCTGGCAAA ATGGAGGCAG AACTGCAGAA AGCCAATAAC 
AAAGTGTGTA ATATGGGTCA CATGCTCAGC CAGCTATCTA TCAAGCTGAA CAATAGTGAA 
AACATGGAGC AGCAGATGGC TTTCCTGAAT AAGCAGCTGC TGCTTTTGGG TGAAGCCAAC 
AAGTTATACA TGGAAGAAAT TGACCGCTTG GGCCCTGAGG CGCACAAGGA GCTGAATATG 
CTGCAAGCAT CTTCAGTGCG GGAGGGTGAG CGTTTGAGGC AGAGCTCTCT TCAGCAGAGT 
CAGAGACTTG AGGCTGCACA GCAGCGTATC ACAGACCTGG AGAACCAACT CACCAAGAAA 
GAGCAAGTCA TCCGGGAGCA AAAGAAACTG CTGGACAATG TCAAACGACA GGCCACGGAG 
CAGCTGCAGG CTTCAGAAAA CAGGTATTTG GCTCAGAAGA ACATCACTCA GGCATTACAA 
ACTGAGCTGC TTGAGCTCTA TAGCAAGATA GAGCTCAAAA ACCTGAAAAC TAATGCACCA 
GCAGAGTCCA TTTCTGATCC CAGCAGTCCC ACCAACCAGA GGCCAAATGG CAACAGTGCA 
GGCCCATCTG CTCCATCTCT ACCGGCTGAC CTGATGAAAA GAGAGGAAGG GGCGCATGTG 
TGTGTCAATG GTGAAATATC TGCAGCGTCT CCTCAAACAC CCACAGCACT CATTAATGGC 
AGTCAGGAAG AAGATCTTTC CGCCCTTAGT CATTCATTTC CCTCCCTCCC CTGCCAACCC 
TTTGCAGTTG GCTCTTACCC CAGCACCAGA AGCTTTTTGG GTAAGAAAGC CAGAGAAATG 
TTCCGCAATA AGAGTGAGAG CCACTATGAT GGCGACTCGC CCACCCTCAC CTGCCTCTCA 
AAGGATCTTA AAGTTGAGCC AATCACAGAC CCCACAGAGA GTTTGGAGTC CGAGGAAGCT 
GCTGACCAGT TTGACCTGCA GCCAATGGAT AGACCCCGGG CATATAATGT TCAGAGTAGG 
AGGCATCAGG ATCTGCGGAT CATGGACTAT AATGAGACCC ATCATGTGCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1A ( XM_005171797.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005171854.1 Danio rerio tuberous sclerosis 1a (tsc1a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa10424
Clone ID Related Accession (Same CDS sequence)	XM_005171797.4 , XM_005171797.3
Accession Version	XM_005171797.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	hamartin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171797.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG AGCAGCCAAG TGTGTTTGAC CTCATCCCTC TTCTGGGGTC CACTGACCTA 
CATGAGCTGG AACAGGTCAA AACCTTGCTG CAGGAAACTC TCAGTGCAGA TAAAGGGACC 
ATGTTGCTGA ATAGCCTTGT AGACTATTTC CTTCAGACAA ACTCATCTCA AGCTGTGGAC 
ATCCTGTCTT CTGTCCGGGA GCCTCATGAT AAGTATCTTT TAGATAAGAT GAATGAATGC 
ATGGGCAAGC AGAGCTGTCG TCTCTCCACC ATCACCCTCC TTGGTCATGT TGTTCGCAAA 
CAGCCACCAT GGATCCACAA GATCGCACGC TTCCCTCTGC TTGTCTCGCT GCTCAAATGC 
CTCAAGACTG ACTCAGATGT AGTAGTTCTC ATAACTGGTG TGCTGGTGCT GATCACTCTA 
ATGCCCATGA TTCCTCAAAC CAACAAACAA CTTCTTTGCG ACTACTTTGA TATCTTTGGA 
CGTCTTGCTG CCTGGAACCA ACGTCATCCA GGCCATGCTC AGGGAGTGTT TGCAGTCCAT 
CTACATGCCA GCGTGTACTC TCTTTTCCAT CGTTTATATG GCATGTATCC TTGTAATTTC 
GTTTCGTACC TGCGTGCCCA TTACAGTATG AAGGAAAATG TTGACACGTT CGAGGAAGTG 
GTGAAGCCCA TGTTGGAACA TGTTCGGATA CATCCTGAGC TCATCACTGG AACCAAAGAC 
TACGAGGTTG ATCCAATTAG ATGGAAGAGA TTTGAGCCAC ATGATATTGT GATTGAATGT 
GCCAAAATTT CACTGGATTC TAAAGAGGCG TCATCTGAAG AGGGCTACTC CTCTTTGGCG 
GATCACATAC AGCGACGCGC TGTACACCAT AGCCAATCAT GTTCAGAGCT CAGTTTCCAT 
GACTCAGGTT CCAGCGCACA GACCCTCAAC AATCAAAGTG ATGAGGTCAA ATCCTCAGGG 
ATGAAGGAGG AGCTGTGGAG CCCTTCCTCC ATATGTGGGA TGAGCACCCC TCCCAGTTCT 
AGAGGAATGT CCCCAACCAC AGTTTCTGAC ATCTCACTTA GTGCCTCACA CCTCTCAGGA 
CGGATCCCCA GCACCCCAGG GGATGGAAAG TGGACTCTAT CTCCCACCAC ACCCTCGGGG 
TCATCCCCTC TCCCATCGCT CTCAGAGGAA ATAGGGCAGG CTGCCCAAAA CACCAGCAGT 
ACCCAGGCTA AGACGTGCAA AGAGGTGAAG AAAGTCACAT CCAGAAGTAT TAATAACAGC 
AGTGAGAATG GAGGTGATGC CAAGACTCCA GTGTCGTTGG CACAGCTCTC TGACATTGTC 
AAAAGGACAG AGGGGGAGCG AGAGGATGAT ACAATCAATG AGGAGATTTT GAGTCTAACC 
CATGGGAAAC TAGACTTTGG ATCTAGGCCT GGTCTGGAAT CCTCATTTTC TGGCTCCTTA 
GACACCCTTC TCTCATCTCA TCCTAATCAT GAGCAGAATT TGGTATCCAC ACCAGACAGG 
GTTGAATGCT CGGCTAAAGC AGGAGAGCAG CAGCTTCCTA GGTCTCTCTG GCCGTTCACC 
CCCATTGAAA ATGGGAAGCA CAGTAAATGT TCTCTGTCTG ATCTCACAAA AGAATCCATG 
CCTTATGAGC CACTGTTTGA CCTCGCCCTG CCGAAGGTGG CATCTCTGTT TTTGGAGAGG 
AAGACTGCTG AGGCCTTTCG GAGGGTTGAA GGAGAAAGAG ATCACCAAGA TGAGATGGAA 
GGAGAGGATC TTTCTGTCAC CTCCCCTCTG GAGGTGTTGG ACCGGGTTAT CCAGCAGGGT 
CACGACACTC ATGGGAAAGT GCTGAAGAGA ACTTTCTCTT CTCCAAGCCA ATCATCAGAC 
CCACACTTGA AAGGTAAACA GCATAGCAAT AAAGGAAAAG GTCTTGTGCC TGCAGTGTCG 
GATGAGCTAG ACATGTTACG CAGTCAATTG CTGCTGCTGC ATAACCAGTT GCTTTATGAG 
AGACATAAGA GAGAGCAACA TGCGCTTCGT AACCGTCGTC TGTTTGGCCG CATCGTCAAC 
ACTACTGCAC TGGAAGAGCA AAATAACTCC ATGAAAACGC AGCTCAAACT TCAGGAAGTT 
GAGATCCAGG CTGTAAAGGT AAGCCTGAAA GAGGAGCAGC GGCGCTCTGT GCACATACAG 
GAAGACAGAG AGGCTGTCGT GAACCAGTTA CAGATTCAAA TTCAGCAGTT ACAGAAGGAG 
CGGAATGACT ACTACTCAAA GATGCAGGTG CTAAAGAGCG AGTTGCAGGA GAATCAGACA 
AAGGCTGGCA AAATGGAGGC AGAACTGCAG AAAGCCAATA ACAAAGTGTG TAATATGGGT 
CACATGCTCA GCCAGCTATC TATCAAGCTG AACAATAGTG AAAACATGGA GCAGCAGATG 
GCTTTCCTGA ATAAGCAGCT GCTGCTTTTG GGTGAAGCCA ACAAGTTATA CATGGAAGAA 
ATTGACCGCT TGGGCCCTGA GGCGCACAAG GAGCTGAATA TGCTGCAAGC ATCTTCAGTG 
CGGGAGGGTG AGCGTTTGAG GCAGAGCTCT CTTCAGCAGA GTCAGAGACT TGAGGCTGCA 
CAGCAGCGTA TCACAGACCT GGAGAACCAA CTCACCAAGA AAGAGCAAGT CATCCGGGAG 
CAAAAGAAAC TGCTGGACAA TGTCAAACGA CAGGCCACGG AGCAGCTGCA GGCTTCAGAA 
AACAGGTATT TGGCTCAGAA GAACATCACT CAGGCATTAC AAACTGAGCT GCTTGAGCTC 
TATAGCAAGA TAGAGCTCAA AAACCTGAAA ACTAATGCAC CAGCAGAGTC CATTTCTGAT 
CCCAGCAGTC CCACCAACCA GAGGCCAAAT GGCAACAGTG CAGGCCCATC TGCTCCATCT 
CTACCGGCTG ACCTGATGAA AAGAGAGGAA GGGGCGCATG TGTGTGTCAA TGGTGAAATA 
TCTGCAGCGT CTCCTCAAAC ACCCACAGCA CTCATTAATG GCAGTCAGGA AGAAGATCTT 
TCCGCCCTTA GTCATTCATT TCCCTCCCTC CCCTGCCAAC CCTTTGCAGT TGGCTCTTAC 
CCCAGCACCA GAAGCTTTTT GGGTAAGAAA GCCAGAGAAA TGTTCCGCAA TAAGAGTGAG 
AGCCACTATG ATGGCGACTC GCCCACCCTC ACCTGCCTCT CAAAGGATCT TAAAGTTGAG 
CCAATCACAG ACCCCACAGA GAGTTTGGAG TCCGAGGAAG CTGCTGACCA GTTTGACCTG 
CAGCCAATGG ATAGACCCCG GGCATATAAT GTTCAGAGTA GGAGGCATCA GGATCTGCGG 
ATCATGGACT ATAATGAGAC CCATCATGTG CATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171854.1
CDS	222..3557
Translation	MAKEQPSVFDLIPLLGSTDLHELEQVKTLLQETLSADKGTMLLNSLVDYFLQTNSSQAVDILSSVREPHDKYLLDKMNECMGKQSCRLSTITLLGHVVRKQPPWIHKIARFPLLVSLLKCLKTDSDVVVLITGVLVLITLMPMIPQTNKQLLCDYFDIFGRLAAWNQRHPGHAQGVFAVHLHASVYSLFHRLYGMYPCNFVSYLRAHYSMKENVDTFEEVVKPMLEHVRIHPELITGTKDYEVDPIRWKRFEPHDIVIECAKISLDSKEASSEEGYSSLADHIQRRAVHHSQSCSELSFHDSGSSAQTLNNQSDEVKSSGMKEELWSPSSICGMSTPPSSRGMSPTTVSDISLSASHLSGRIPSTPGDGKWTLSPTTPSGSSPLPSLSEEIGQAAQNTSSTQAKTCKEVKKVTSRSINNSSENGGDAKTPVSLAQLSDIVKRTEGEREDDTINEEILSLTHGKLDFGSRPGLESSFSGSLDTLLSSHPNHEQNLVSTPDRVECSAKAGEQQLPRSLWPFTPIENGKHSKCSLSDLTKESMPYEPLFDLALPKVASLFLERKTAEAFRRVEGERDHQDEMEGEDLSVTSPLEVLDRVIQQGHDTHGKVLKRTFSSPSQSSDPHLKGKQHSNKGKGLVPAVSDELDMLRSQLLLLHNQLLYERHKREQHALRNRRLFGRIVNTTALEEQNNSMKTQLKLQEVEIQAVKVSLKEEQRRSVHIQEDREAVVNQLQIQIQQLQKERNDYYSKMQVLKSELQENQTKAGKMEAELQKANNKVCNMGHMLSQLSIKLNNSENMEQQMAFLNKQLLLLGEANKLYMEEIDRLGPEAHKELNMLQASSVREGERLRQSSLQQSQRLEAAQQRITDLENQLTKKEQVIREQKKLLDNVKRQATEQLQASENRYLAQKNITQALQTELLELYSKIELKNLKTNAPAESISDPSSPTNQRPNGNSAGPSAPSLPADLMKREEGAHVCVNGEISAASPQTPTALINGSQEEDLSALSHSFPSLPCQPFAVGSYPSTRSFLGKKAREMFRNKSESHYDGDSPTLTCLSKDLKVEPITDPTESLESEEAADQFDLQPMDRPRAYNVQSRRHQDLRIMDYNETHHVH

Target ORF information:

RefSeq Version	XM_005171797.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tuberous sclerosis 1a (tsc1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171797.4

ORF Insert Sequence:

ATGGCCAAAG AGCAGCCAAG TGTGTTTGAC CTCATCCCTC TTCTGGGGTC CACTGACCTA 
CATGAGCTGG AACAGGTCAA AACCTTGCTG CAGGAAACTC TCAGTGCAGA TAAAGGGACC 
ATGTTGCTGA ATAGCCTTGT AGACTATTTC CTTCAGACAA ACTCATCTCA AGCTGTGGAC 
ATCCTGTCTT CTGTCCGGGA GCCTCATGAT AAGTATCTTT TAGATAAGAT GAATGAATGC 
ATGGGCAAGC AGAGCTGTCG TCTCTCCACC ATCACCCTCC TTGGTCATGT TGTTCGCAAA 
CAGCCACCAT GGATCCACAA GATCGCACGC TTCCCTCTGC TTGTCTCGCT GCTCAAATGC 
CTCAAGACTG ACTCAGATGT AGTAGTTCTC ATAACTGGTG TGCTGGTGCT GATCACTCTA 
ATGCCCATGA TTCCTCAAAC CAACAAACAA CTTCTTTGCG ACTACTTTGA TATCTTTGGA 
CGTCTTGCTG CCTGGAACCA ACGTCATCCA GGCCATGCTC AGGGAGTGTT TGCAGTCCAT 
CTACATGCCA GCGTGTACTC TCTTTTCCAT CGTTTATATG GCATGTATCC TTGTAATTTC 
GTTTCGTACC TGCGTGCCCA TTACAGTATG AAGGAAAATG TTGACACGTT CGAGGAAGTG 
GTGAAGCCCA TGTTGGAACA TGTTCGGATA CATCCTGAGC TCATCACTGG AACCAAAGAC 
TACGAGGTTG ATCCAATTAG ATGGAAGAGA TTTGAGCCAC ATGATATTGT GATTGAATGT 
GCCAAAATTT CACTGGATTC TAAAGAGGCG TCATCTGAAG AGGGCTACTC CTCTTTGGCG 
GATCACATAC AGCGACGCGC TGTACACCAT AGCCAATCAT GTTCAGAGCT CAGTTTCCAT 
GACTCAGGTT CCAGCGCACA GACCCTCAAC AATCAAAGTG ATGAGGTCAA ATCCTCAGGG 
ATGAAGGAGG AGCTGTGGAG CCCTTCCTCC ATATGTGGGA TGAGCACCCC TCCCAGTTCT 
AGAGGAATGT CCCCAACCAC AGTTTCTGAC ATCTCACTTA GTGCCTCACA CCTCTCAGGA 
CGGATCCCCA GCACCCCAGG GGATGGAAAG TGGACTCTAT CTCCCACCAC ACCCTCGGGG 
TCATCCCCTC TCCCATCGCT CTCAGAGGAA ATAGGGCAGG CTGCCCAAAA CACCAGCAGT 
ACCCAGGCTA AGACGTGCAA AGAGGTGAAG AAAGTCACAT CCAGAAGTAT TAATAACAGC 
AGTGAGAATG GAGGTGATGC CAAGACTCCA GTGTCGTTGG CACAGCTCTC TGACATTGTC 
AAAAGGACAG AGGGGGAGCG AGAGGATGAT ACAATCAATG AGGAGATTTT GAGTCTAACC 
CATGGGAAAC TAGACTTTGG ATCTAGGCCT GGTCTGGAAT CCTCATTTTC TGGCTCCTTA 
GACACCCTTC TCTCATCTCA TCCTAATCAT GAGCAGAATT TGGTATCCAC ACCAGACAGG 
GTTGAATGCT CGGCTAAAGC AGGAGAGCAG CAGCTTCCTA GGTCTCTCTG GCCGTTCACC 
CCCATTGAAA ATGGGAAGCA CAGTAAATGT TCTCTGTCTG ATCTCACAAA AGAATCCATG 
CCTTATGAGC CACTGTTTGA CCTCGCCCTG CCGAAGGTGG CATCTCTGTT TTTGGAGAGG 
AAGACTGCTG AGGCCTTTCG GAGGGTTGAA GGAGAAAGAG ATCACCAAGA TGAGATGGAA 
GGAGAGGATC TTTCTGTCAC CTCCCCTCTG GAGGTGTTGG ACCGGGTTAT CCAGCAGGGT 
CACGACACTC ATGGGAAAGT GCTGAAGAGA ACTTTCTCTT CTCCAAGCCA ATCATCAGAC 
CCACACTTGA AAGGTAAACA GCATAGCAAT AAAGGAAAAG GTCTTGTGCC TGCAGTGTCG 
GATGAGCTAG ACATGTTACG CAGTCAATTG CTGCTGCTGC ATAACCAGTT GCTTTATGAG 
AGACATAAGA GAGAGCAACA TGCGCTTCGT AACCGTCGTC TGTTTGGCCG CATCGTCAAC 
ACTACTGCAC TGGAAGAGCA AAATAACTCC ATGAAAACGC AGCTCAAACT TCAGGAAGTT 
GAGATCCAGG CTGTAAAGGT AAGCCTGAAA GAGGAGCAGC GGCGCTCTGT GCACATACAG 
GAAGACAGAG AGGCTGTCGT GAACCAGTTA CAGATTCAAA TTCAGCAGTT ACAGAAGGAG 
CGGAATGACT ACTACTCAAA GATGCAGGTG CTAAAGAGCG AGTTGCAGGA GAATCAGACA 
AAGGCTGGCA AAATGGAGGC AGAACTGCAG AAAGCCAATA ACAAAGTGTG TAATATGGGT 
CACATGCTCA GCCAGCTATC TATCAAGCTG AACAATAGTG AAAACATGGA GCAGCAGATG 
GCTTTCCTGA ATAAGCAGCT GCTGCTTTTG GGTGAAGCCA ACAAGTTATA CATGGAAGAA 
ATTGACCGCT TGGGCCCTGA GGCGCACAAG GAGCTGAATA TGCTGCAAGC ATCTTCAGTG 
CGGGAGGGTG AGCGTTTGAG GCAGAGCTCT CTTCAGCAGA GTCAGAGACT TGAGGCTGCA 
CAGCAGCGTA TCACAGACCT GGAGAACCAA CTCACCAAGA AAGAGCAAGT CATCCGGGAG 
CAAAAGAAAC TGCTGGACAA TGTCAAACGA CAGGCCACGG AGCAGCTGCA GGCTTCAGAA 
AACAGGTATT TGGCTCAGAA GAACATCACT CAGGCATTAC AAACTGAGCT GCTTGAGCTC 
TATAGCAAGA TAGAGCTCAA AAACCTGAAA ACTAATGCAC CAGCAGAGTC CATTTCTGAT 
CCCAGCAGTC CCACCAACCA GAGGCCAAAT GGCAACAGTG CAGGCCCATC TGCTCCATCT 
CTACCGGCTG ACCTGATGAA AAGAGAGGAA GGGGCGCATG TGTGTGTCAA TGGTGAAATA 
TCTGCAGCGT CTCCTCAAAC ACCCACAGCA CTCATTAATG GCAGTCAGGA AGAAGATCTT 
TCCGCCCTTA GTCATTCATT TCCCTCCCTC CCCTGCCAAC CCTTTGCAGT TGGCTCTTAC 
CCCAGCACCA GAAGCTTTTT GGGTAAGAAA GCCAGAGAAA TGTTCCGCAA TAAGAGTGAG 
AGCCACTATG ATGGCGACTC GCCCACCCTC ACCTGCCTCT CAAAGGATCT TAAAGTTGAG 
CCAATCACAG ACCCCACAGA GAGTTTGGAG TCCGAGGAAG CTGCTGACCA GTTTGACCTG 
CAGCCAATGG ATAGACCCCG GGCATATAAT GTTCAGAGTA GGAGGCATCA GGATCTGCGG 
ATCATGGACT ATAATGAGAC CCATCATGTG CATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1A ( XM_005171797.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005171854.1 Danio rerio tuberous sclerosis 1a (tsc1a), transcript variant X3, mRNA.

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CloneID	ODa10424
Clone ID Related Accession (Same CDS sequence)	XM_005171797.4 , XM_005171797.3
Accession Version	XM_005171797.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	hamartin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171797.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG AGCAGCCAAG TGTGTTTGAC CTCATCCCTC TTCTGGGGTC CACTGACCTA 
CATGAGCTGG AACAGGTCAA AACCTTGCTG CAGGAAACTC TCAGTGCAGA TAAAGGGACC 
ATGTTGCTGA ATAGCCTTGT AGACTATTTC CTTCAGACAA ACTCATCTCA AGCTGTGGAC 
ATCCTGTCTT CTGTCCGGGA GCCTCATGAT AAGTATCTTT TAGATAAGAT GAATGAATGC 
ATGGGCAAGC AGAGCTGTCG TCTCTCCACC ATCACCCTCC TTGGTCATGT TGTTCGCAAA 
CAGCCACCAT GGATCCACAA GATCGCACGC TTCCCTCTGC TTGTCTCGCT GCTCAAATGC 
CTCAAGACTG ACTCAGATGT AGTAGTTCTC ATAACTGGTG TGCTGGTGCT GATCACTCTA 
ATGCCCATGA TTCCTCAAAC CAACAAACAA CTTCTTTGCG ACTACTTTGA TATCTTTGGA 
CGTCTTGCTG CCTGGAACCA ACGTCATCCA GGCCATGCTC AGGGAGTGTT TGCAGTCCAT 
CTACATGCCA GCGTGTACTC TCTTTTCCAT CGTTTATATG GCATGTATCC TTGTAATTTC 
GTTTCGTACC TGCGTGCCCA TTACAGTATG AAGGAAAATG TTGACACGTT CGAGGAAGTG 
GTGAAGCCCA TGTTGGAACA TGTTCGGATA CATCCTGAGC TCATCACTGG AACCAAAGAC 
TACGAGGTTG ATCCAATTAG ATGGAAGAGA TTTGAGCCAC ATGATATTGT GATTGAATGT 
GCCAAAATTT CACTGGATTC TAAAGAGGCG TCATCTGAAG AGGGCTACTC CTCTTTGGCG 
GATCACATAC AGCGACGCGC TGTACACCAT AGCCAATCAT GTTCAGAGCT CAGTTTCCAT 
GACTCAGGTT CCAGCGCACA GACCCTCAAC AATCAAAGTG ATGAGGTCAA ATCCTCAGGG 
ATGAAGGAGG AGCTGTGGAG CCCTTCCTCC ATATGTGGGA TGAGCACCCC TCCCAGTTCT 
AGAGGAATGT CCCCAACCAC AGTTTCTGAC ATCTCACTTA GTGCCTCACA CCTCTCAGGA 
CGGATCCCCA GCACCCCAGG GGATGGAAAG TGGACTCTAT CTCCCACCAC ACCCTCGGGG 
TCATCCCCTC TCCCATCGCT CTCAGAGGAA ATAGGGCAGG CTGCCCAAAA CACCAGCAGT 
ACCCAGGCTA AGACGTGCAA AGAGGTGAAG AAAGTCACAT CCAGAAGTAT TAATAACAGC 
AGTGAGAATG GAGGTGATGC CAAGACTCCA GTGTCGTTGG CACAGCTCTC TGACATTGTC 
AAAAGGACAG AGGGGGAGCG AGAGGATGAT ACAATCAATG AGGAGATTTT GAGTCTAACC 
CATGGGAAAC TAGACTTTGG ATCTAGGCCT GGTCTGGAAT CCTCATTTTC TGGCTCCTTA 
GACACCCTTC TCTCATCTCA TCCTAATCAT GAGCAGAATT TGGTATCCAC ACCAGACAGG 
GTTGAATGCT CGGCTAAAGC AGGAGAGCAG CAGCTTCCTA GGTCTCTCTG GCCGTTCACC 
CCCATTGAAA ATGGGAAGCA CAGTAAATGT TCTCTGTCTG ATCTCACAAA AGAATCCATG 
CCTTATGAGC CACTGTTTGA CCTCGCCCTG CCGAAGGTGG CATCTCTGTT TTTGGAGAGG 
AAGACTGCTG AGGCCTTTCG GAGGGTTGAA GGAGAAAGAG ATCACCAAGA TGAGATGGAA 
GGAGAGGATC TTTCTGTCAC CTCCCCTCTG GAGGTGTTGG ACCGGGTTAT CCAGCAGGGT 
CACGACACTC ATGGGAAAGT GCTGAAGAGA ACTTTCTCTT CTCCAAGCCA ATCATCAGAC 
CCACACTTGA AAGGTAAACA GCATAGCAAT AAAGGAAAAG GTCTTGTGCC TGCAGTGTCG 
GATGAGCTAG ACATGTTACG CAGTCAATTG CTGCTGCTGC ATAACCAGTT GCTTTATGAG 
AGACATAAGA GAGAGCAACA TGCGCTTCGT AACCGTCGTC TGTTTGGCCG CATCGTCAAC 
ACTACTGCAC TGGAAGAGCA AAATAACTCC ATGAAAACGC AGCTCAAACT TCAGGAAGTT 
GAGATCCAGG CTGTAAAGGT AAGCCTGAAA GAGGAGCAGC GGCGCTCTGT GCACATACAG 
GAAGACAGAG AGGCTGTCGT GAACCAGTTA CAGATTCAAA TTCAGCAGTT ACAGAAGGAG 
CGGAATGACT ACTACTCAAA GATGCAGGTG CTAAAGAGCG AGTTGCAGGA GAATCAGACA 
AAGGCTGGCA AAATGGAGGC AGAACTGCAG AAAGCCAATA ACAAAGTGTG TAATATGGGT 
CACATGCTCA GCCAGCTATC TATCAAGCTG AACAATAGTG AAAACATGGA GCAGCAGATG 
GCTTTCCTGA ATAAGCAGCT GCTGCTTTTG GGTGAAGCCA ACAAGTTATA CATGGAAGAA 
ATTGACCGCT TGGGCCCTGA GGCGCACAAG GAGCTGAATA TGCTGCAAGC ATCTTCAGTG 
CGGGAGGGTG AGCGTTTGAG GCAGAGCTCT CTTCAGCAGA GTCAGAGACT TGAGGCTGCA 
CAGCAGCGTA TCACAGACCT GGAGAACCAA CTCACCAAGA AAGAGCAAGT CATCCGGGAG 
CAAAAGAAAC TGCTGGACAA TGTCAAACGA CAGGCCACGG AGCAGCTGCA GGCTTCAGAA 
AACAGGTATT TGGCTCAGAA GAACATCACT CAGGCATTAC AAACTGAGCT GCTTGAGCTC 
TATAGCAAGA TAGAGCTCAA AAACCTGAAA ACTAATGCAC CAGCAGAGTC CATTTCTGAT 
CCCAGCAGTC CCACCAACCA GAGGCCAAAT GGCAACAGTG CAGGCCCATC TGCTCCATCT 
CTACCGGCTG ACCTGATGAA AAGAGAGGAA GGGGCGCATG TGTGTGTCAA TGGTGAAATA 
TCTGCAGCGT CTCCTCAAAC ACCCACAGCA CTCATTAATG GCAGTCAGGA AGAAGATCTT 
TCCGCCCTTA GTCATTCATT TCCCTCCCTC CCCTGCCAAC CCTTTGCAGT TGGCTCTTAC 
CCCAGCACCA GAAGCTTTTT GGGTAAGAAA GCCAGAGAAA TGTTCCGCAA TAAGAGTGAG 
AGCCACTATG ATGGCGACTC GCCCACCCTC ACCTGCCTCT CAAAGGATCT TAAAGTTGAG 
CCAATCACAG ACCCCACAGA GAGTTTGGAG TCCGAGGAAG CTGCTGACCA GTTTGACCTG 
CAGCCAATGG ATAGACCCCG GGCATATAAT GTTCAGAGTA GGAGGCATCA GGATCTGCGG 
ATCATGGACT ATAATGAGAC CCATCATGTG CATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171854.1
CDS	217..3552
Translation	MAKEQPSVFDLIPLLGSTDLHELEQVKTLLQETLSADKGTMLLNSLVDYFLQTNSSQAVDILSSVREPHDKYLLDKMNECMGKQSCRLSTITLLGHVVRKQPPWIHKIARFPLLVSLLKCLKTDSDVVVLITGVLVLITLMPMIPQTNKQLLCDYFDIFGRLAAWNQRHPGHAQGVFAVHLHASVYSLFHRLYGMYPCNFVSYLRAHYSMKENVDTFEEVVKPMLEHVRIHPELITGTKDYEVDPIRWKRFEPHDIVIECAKISLDSKEASSEEGYSSLADHIQRRAVHHSQSCSELSFHDSGSSAQTLNNQSDEVKSSGMKEELWSPSSICGMSTPPSSRGMSPTTVSDISLSASHLSGRIPSTPGDGKWTLSPTTPSGSSPLPSLSEEIGQAAQNTSSTQAKTCKEVKKVTSRSINNSSENGGDAKTPVSLAQLSDIVKRTEGEREDDTINEEILSLTHGKLDFGSRPGLESSFSGSLDTLLSSHPNHEQNLVSTPDRVECSAKAGEQQLPRSLWPFTPIENGKHSKCSLSDLTKESMPYEPLFDLALPKVASLFLERKTAEAFRRVEGERDHQDEMEGEDLSVTSPLEVLDRVIQQGHDTHGKVLKRTFSSPSQSSDPHLKGKQHSNKGKGLVPAVSDELDMLRSQLLLLHNQLLYERHKREQHALRNRRLFGRIVNTTALEEQNNSMKTQLKLQEVEIQAVKVSLKEEQRRSVHIQEDREAVVNQLQIQIQQLQKERNDYYSKMQVLKSELQENQTKAGKMEAELQKANNKVCNMGHMLSQLSIKLNNSENMEQQMAFLNKQLLLLGEANKLYMEEIDRLGPEAHKELNMLQASSVREGERLRQSSLQQSQRLEAAQQRITDLENQLTKKEQVIREQKKLLDNVKRQATEQLQASENRYLAQKNITQALQTELLELYSKIELKNLKTNAPAESISDPSSPTNQRPNGNSAGPSAPSLPADLMKREEGAHVCVNGEISAASPQTPTALINGSQEEDLSALSHSFPSLPCQPFAVGSYPSTRSFLGKKAREMFRNKSESHYDGDSPTLTCLSKDLKVEPITDPTESLESEEAADQFDLQPMDRPRAYNVQSRRHQDLRIMDYNETHHVH

Target ORF information:

RefSeq Version	XM_005171797.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tuberous sclerosis 1a (tsc1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171797.3

ORF Insert Sequence:

ATGGCCAAAG AGCAGCCAAG TGTGTTTGAC CTCATCCCTC TTCTGGGGTC CACTGACCTA 
CATGAGCTGG AACAGGTCAA AACCTTGCTG CAGGAAACTC TCAGTGCAGA TAAAGGGACC 
ATGTTGCTGA ATAGCCTTGT AGACTATTTC CTTCAGACAA ACTCATCTCA AGCTGTGGAC 
ATCCTGTCTT CTGTCCGGGA GCCTCATGAT AAGTATCTTT TAGATAAGAT GAATGAATGC 
ATGGGCAAGC AGAGCTGTCG TCTCTCCACC ATCACCCTCC TTGGTCATGT TGTTCGCAAA 
CAGCCACCAT GGATCCACAA GATCGCACGC TTCCCTCTGC TTGTCTCGCT GCTCAAATGC 
CTCAAGACTG ACTCAGATGT AGTAGTTCTC ATAACTGGTG TGCTGGTGCT GATCACTCTA 
ATGCCCATGA TTCCTCAAAC CAACAAACAA CTTCTTTGCG ACTACTTTGA TATCTTTGGA 
CGTCTTGCTG CCTGGAACCA ACGTCATCCA GGCCATGCTC AGGGAGTGTT TGCAGTCCAT 
CTACATGCCA GCGTGTACTC TCTTTTCCAT CGTTTATATG GCATGTATCC TTGTAATTTC 
GTTTCGTACC TGCGTGCCCA TTACAGTATG AAGGAAAATG TTGACACGTT CGAGGAAGTG 
GTGAAGCCCA TGTTGGAACA TGTTCGGATA CATCCTGAGC TCATCACTGG AACCAAAGAC 
TACGAGGTTG ATCCAATTAG ATGGAAGAGA TTTGAGCCAC ATGATATTGT GATTGAATGT 
GCCAAAATTT CACTGGATTC TAAAGAGGCG TCATCTGAAG AGGGCTACTC CTCTTTGGCG 
GATCACATAC AGCGACGCGC TGTACACCAT AGCCAATCAT GTTCAGAGCT CAGTTTCCAT 
GACTCAGGTT CCAGCGCACA GACCCTCAAC AATCAAAGTG ATGAGGTCAA ATCCTCAGGG 
ATGAAGGAGG AGCTGTGGAG CCCTTCCTCC ATATGTGGGA TGAGCACCCC TCCCAGTTCT 
AGAGGAATGT CCCCAACCAC AGTTTCTGAC ATCTCACTTA GTGCCTCACA CCTCTCAGGA 
CGGATCCCCA GCACCCCAGG GGATGGAAAG TGGACTCTAT CTCCCACCAC ACCCTCGGGG 
TCATCCCCTC TCCCATCGCT CTCAGAGGAA ATAGGGCAGG CTGCCCAAAA CACCAGCAGT 
ACCCAGGCTA AGACGTGCAA AGAGGTGAAG AAAGTCACAT CCAGAAGTAT TAATAACAGC 
AGTGAGAATG GAGGTGATGC CAAGACTCCA GTGTCGTTGG CACAGCTCTC TGACATTGTC 
AAAAGGACAG AGGGGGAGCG AGAGGATGAT ACAATCAATG AGGAGATTTT GAGTCTAACC 
CATGGGAAAC TAGACTTTGG ATCTAGGCCT GGTCTGGAAT CCTCATTTTC TGGCTCCTTA 
GACACCCTTC TCTCATCTCA TCCTAATCAT GAGCAGAATT TGGTATCCAC ACCAGACAGG 
GTTGAATGCT CGGCTAAAGC AGGAGAGCAG CAGCTTCCTA GGTCTCTCTG GCCGTTCACC 
CCCATTGAAA ATGGGAAGCA CAGTAAATGT TCTCTGTCTG ATCTCACAAA AGAATCCATG 
CCTTATGAGC CACTGTTTGA CCTCGCCCTG CCGAAGGTGG CATCTCTGTT TTTGGAGAGG 
AAGACTGCTG AGGCCTTTCG GAGGGTTGAA GGAGAAAGAG ATCACCAAGA TGAGATGGAA 
GGAGAGGATC TTTCTGTCAC CTCCCCTCTG GAGGTGTTGG ACCGGGTTAT CCAGCAGGGT 
CACGACACTC ATGGGAAAGT GCTGAAGAGA ACTTTCTCTT CTCCAAGCCA ATCATCAGAC 
CCACACTTGA AAGGTAAACA GCATAGCAAT AAAGGAAAAG GTCTTGTGCC TGCAGTGTCG 
GATGAGCTAG ACATGTTACG CAGTCAATTG CTGCTGCTGC ATAACCAGTT GCTTTATGAG 
AGACATAAGA GAGAGCAACA TGCGCTTCGT AACCGTCGTC TGTTTGGCCG CATCGTCAAC 
ACTACTGCAC TGGAAGAGCA AAATAACTCC ATGAAAACGC AGCTCAAACT TCAGGAAGTT 
GAGATCCAGG CTGTAAAGGT AAGCCTGAAA GAGGAGCAGC GGCGCTCTGT GCACATACAG 
GAAGACAGAG AGGCTGTCGT GAACCAGTTA CAGATTCAAA TTCAGCAGTT ACAGAAGGAG 
CGGAATGACT ACTACTCAAA GATGCAGGTG CTAAAGAGCG AGTTGCAGGA GAATCAGACA 
AAGGCTGGCA AAATGGAGGC AGAACTGCAG AAAGCCAATA ACAAAGTGTG TAATATGGGT 
CACATGCTCA GCCAGCTATC TATCAAGCTG AACAATAGTG AAAACATGGA GCAGCAGATG 
GCTTTCCTGA ATAAGCAGCT GCTGCTTTTG GGTGAAGCCA ACAAGTTATA CATGGAAGAA 
ATTGACCGCT TGGGCCCTGA GGCGCACAAG GAGCTGAATA TGCTGCAAGC ATCTTCAGTG 
CGGGAGGGTG AGCGTTTGAG GCAGAGCTCT CTTCAGCAGA GTCAGAGACT TGAGGCTGCA 
CAGCAGCGTA TCACAGACCT GGAGAACCAA CTCACCAAGA AAGAGCAAGT CATCCGGGAG 
CAAAAGAAAC TGCTGGACAA TGTCAAACGA CAGGCCACGG AGCAGCTGCA GGCTTCAGAA 
AACAGGTATT TGGCTCAGAA GAACATCACT CAGGCATTAC AAACTGAGCT GCTTGAGCTC 
TATAGCAAGA TAGAGCTCAA AAACCTGAAA ACTAATGCAC CAGCAGAGTC CATTTCTGAT 
CCCAGCAGTC CCACCAACCA GAGGCCAAAT GGCAACAGTG CAGGCCCATC TGCTCCATCT 
CTACCGGCTG ACCTGATGAA AAGAGAGGAA GGGGCGCATG TGTGTGTCAA TGGTGAAATA 
TCTGCAGCGT CTCCTCAAAC ACCCACAGCA CTCATTAATG GCAGTCAGGA AGAAGATCTT 
TCCGCCCTTA GTCATTCATT TCCCTCCCTC CCCTGCCAAC CCTTTGCAGT TGGCTCTTAC 
CCCAGCACCA GAAGCTTTTT GGGTAAGAAA GCCAGAGAAA TGTTCCGCAA TAAGAGTGAG 
AGCCACTATG ATGGCGACTC GCCCACCCTC ACCTGCCTCT CAAAGGATCT TAAAGTTGAG 
CCAATCACAG ACCCCACAGA GAGTTTGGAG TCCGAGGAAG CTGCTGACCA GTTTGACCTG 
CAGCCAATGG ATAGACCCCG GGCATATAAT GTTCAGAGTA GGAGGCATCA GGATCTGCGG 
ATCATGGACT ATAATGAGAC CCATCATGTG CATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Heat-induced masculinization in domesticated zebrafish is family-specific and yields a set of different gonadal transcriptomes.
Proceedings of the National Academy of Sciences of the United States of America114(6)E941-E950(2017 Feb)
Ribas L,Liew WC,D?az N,Sreenivasan R,Orb?n L,Piferrer F

Transcriptomic profiling of mTOR and ryanodine receptor signaling molecules in developing zebrafish in the absence and presence of PCB 95.
PeerJ5e4106(2017)
Frank DF,Miller GW,Connon RE,Geist J,Lein PJ

Maternal exposure to the water soluble fraction of crude oil, lead and their mixture induces autism-like behavioral deficits in zebrafish (Danio rerio) larvae.
Ecotoxicology and environmental safety134P123-30(2016 Dec)
Wang Y,Zhong H,Wang C,Gao D,Zhou Y,Zuo Z

Genome wide analysis reveals Zic3 interaction with distal regulatory elements of stage specific developmental genes in zebrafish.
PLoS genetics9(10)e1003852(2013 Oct)
Winata CL,Kondrychyn I,Kumar V,Srinivasan KG,Orlov Y,Ravishankar A,Prabhakar S,Stanton LW,Korzh V,Mathavan S

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TSC1A cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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