Tsc1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tsc1
Entrez Gene ID	60445
Full Name	TSC complex subunit 1
General protein information	Preferred Names TSC complex subunit 1 Names hamartin tuberous sclerosis 1 protein homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3p12

mRNA and Protein(s)

mRNA	Protein	Name
XM_017592016.1	XP_017447505.1	hamartin isoform X3
XM_006233844.2	XP_006233906.1	hamartin isoform X1
XM_006233846.2	XP_006233908.1	hamartin isoform X1
XM_006233845.2	XP_006233907.1	hamartin isoform X1
XM_006233849.2	XP_006233911.1	hamartin isoform X2
NM_021854.1	NP_068626.1	hamartin
XM_006233847.3	XP_006233909.1	hamartin isoform X1
XM_006233843.2	XP_006233905.1	hamartin isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TSC1	NP_000359.1
Bos taurus (cattle)	TSC1	XP_005213508.1
	TSC1	XP_001103846.2
Mus musculus (house mouse)	Tsc1	NP_075025.2
Xenopus tropicalis (tropical clawed frog)	tsc1	XP_002944681.2
Pan troglodytes (chimpanzee)	TSC1	XP_001168992.1
Gallus gallus (chicken)	TSC1	XP_415449.2
Canis lupus familiaris (dog)	TSC1	XP_005625233.1
Rattus norvegicus (Norway rat)	Tsc1	NP_068626.1
Danio rerio (zebrafish)	tsc1a	NP_956346.1

Gene Ontology
Bibliography

Related articles in PubMed

Tumor suppressor Tsc1 is a new Hsp90 co-chaperone that facilitates folding of kinase and non-kinase clients.
Woodford MR, Sager RA, Marris E, Dunn DM, Blanden AR, Murphy RL, Rensing N, Shapiro O, Panaretou B, Prodromou C, Loh SN, Gutmann DH, Bourboulia D, Bratslavsky G, Wong M, Mollapour M
The EMBO journal36(24)3650-3665(2017 12)

Somatic Mutations in TSC1 and TSC2 Cause Focal Cortical Dysplasia.
Lim JS, Gopalappa R, Kim SH, Ramakrishna S, Lee M, Kim WI, Kim J, Park SM, Lee J, Oh JH, Kim HD, Park CH, Lee JS, Kim S, Kim DS, Han JM, Kang HC, Kim HH, Lee JH
American journal of human genetics100(3)454-472(2017 Mar)

Intellectual ability in tuberous sclerosis complex correlates with predicted effects of mutations on TSC1 and TSC2 proteins.
Wong HT, McCartney DL, Lewis JC, Sampson JR, Howe CJ, de Vries PJ
Journal of medical genetics52(12)815-22(2015 Dec)

Survival benefit and phenotypic improvement by hamartin gene therapy in a tuberous sclerosis mouse brain model.
Prabhakar S, Zhang X, Goto J, Han S, Lai C, Bronson R, Sena-Esteves M, Ramesh V, Stemmer-Rachamimov A, Kwiatkowski DJ, Breakefield XO
Neurobiology of disease8222-31(2015 Oct)

Regulation of mTORC1 by PI3K signaling.
Dibble CC, Cantley LC
Trends in cell biology25(9)545-55(2015 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TSC1 is selectively induced by ischemia in hippocampal CA3 neurons; in CA1 neurons, TSC1 was unaffected by ischemia but was upregulated by ischemic preconditioning preceding ischemia; overexpression of TSC1 increased resistance to oxygen glucose deprivation by inducing productive autophagy
Title: Tsc1 (hamartin) confers neuroprotection against ischemia by inducing autophagy.

Hamartin-Hsp70 plays a crucial role as a scaffolding protein that transfers the Akt signal to tuberin.
Title: Hamartin-Hsp70 interaction is necessary for Akt-dependent tuberin phosphorylation during heat shock.

findings show that endoplasmic reticulum (ER) stress regulates TSC1/TSC2 complex to limit mTOR activity
Title: Tuberous sclerosis complex activity is required to control neuronal stress responses in an mTOR-dependent manner.

These data strongly suggest the effects of Akt/TSC1-TSC2/mTOR signal pathway on hepatocyte growth, protein synthesis and cell cycle, and prove its contribution to liver regeneration.
Title: The variation of AkT/TSC1-TSC1/mTOR signal pathway in hepatocytes after partial hepatectomy in rats.

The TSC1 is thought to be involved in protein translational regulation and cell growth, and loss of their function is a cause of TSC and lymphangioleiomyomatosis (LAM).
Title: TSC2 modulates actin cytoskeleton and focal adhesion through TSC1-binding domain and the Rac1 GTPase.

The following Tsc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tsc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa60529	XM_017592016.1 Latest version!	Rattus norvegicus tuberous sclerosis 1 (Tsc1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa05290	NM_021854.1 Latest version!	Rattus norvegicus TSC complex subunit 1 (Tsc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa25661	XM_006233844.2 Latest version!	Rattus norvegicus tuberous sclerosis 1 (Tsc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa25661	XM_006233845.2 Latest version!	Rattus norvegicus tuberous sclerosis 1 (Tsc1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa25661	XM_006233847.3 Latest version!	Rattus norvegicus tuberous sclerosis 1 (Tsc1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa25661	XM_006233846.2 Latest version!	Rattus norvegicus tuberous sclerosis 1 (Tsc1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa25661	XM_006233843.2 Latest version!	Rattus norvegicus tuberous sclerosis 1 (Tsc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa25662	XM_006233849.2 Latest version!	Rattus norvegicus tuberous sclerosis 1 (Tsc1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tsc1 ( XM_017592016.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447505.1 Rattus norvegicus tuberous sclerosis 1 (Tsc1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ORa60529
Clone ID Related Accession (Same CDS sequence)	XM_017592016.1
Accession Version	XM_017592016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	hamartin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTG ATGTTGTGGT TCTCACAACT GGTGTCTTGG TGTTGATCAC CATGCTACCG 
ATGATCCCGC AGTCAGGGAA GCAGCACCTT CTCGACTTCT TTGACATCTT TGGCCGTCTT 
TCATCATGGT GCCTGAAGAA ACCAGGCCAT GTGACAGAAG TCTACCTGGT CCACCTCCAT 
GCCAGTGTTT ATGCCCTCTT CCATCGCCTT TATGGAATGT ACCCTTGTAA CTTCGTCTCC 
TTCCTGCGCT CTCACTACAG CATGAAGGAA AACGTGGAGA CTTTTGAAGA AGTGGTCAAG 
CCAATGATGG AGCATGTGCG AATTCACCCG GAATTAGTGA CTGGATCCAA GGACCATGAA 
CTGGACCCTC GAAGGTGGAA GACATTAGAA ACTCATGATG TTGTAATAGA GTGTGCCAAA 
ATCTCTCTGG ACCCTACCGA AGCCTCATAT GAAGATGGCG ATGCTGTGTC TCACCAGCTC 
TCTGCCTGCT TCCCTCACCG TTCAGCTGAT GTCACCACCA GCTCTTACGT GGACACACAG 
AATAGCTATG GGGGCGCTAC TTCCACCCCT TCCTCCACCT CTCGGCTGAT GTTGTTCAGT 
ACCCCCGGGC AGCTACCTCA GAGTTTGAGT TCACTATCAA CACGGCCGTT ACCTGAGCCG 
CTGCAAGCTA GTCTTTGGAG CCCATCTGCA GTCTGTGGTA TGACCACTCC TCCTACGTCT 
CCTGGAAATG TCCCGGCTGA TTTGTCACAT CCATACAGTA AAGCCTTTGG TACTACCACT 
GGTGGAAAAG GAACTCCTTC AGGAACCCCA GCGACCTCTC CTCCCCCAGC CCCGCCTTGT 
CCCCAAGACG ACTGTGCGCA CGGTCCAGCC TCCCAGGCCT CAGCCACACC CCCCAGGAAG 
GAAGAAAGAG CAGATTCTTC AAGGCCTTAC CTACCCAGAC AGCAGGATGT TCCGAGTGAC 
CGCGGATTAG AGGATCTACC TGGAAGCAAA GGTTCCGTCA CTCTGAGGAA TCTACCAGAT 
TTCCTAGGTG ACCTGGCTTC AGAGGAAGAC AGTATAGAGA AAGATAAGGA AGAAGCTGCG 
ATATCTAAAG AGCTTTCTGA GATCACCACT GCAGAGGCGG ATCCTGTAGC TCCTCGAGGG 
GGCTTTGACT CTCCCTTCTA CCGAGACAGT CTCTCTGGTT CTCAGCGGAA GACTCACTCA 
GCAGCCTCTG GGACTCAGGG CTTCAGCGTG AACCCTGAGC CTTTGCACTC CTCCCTGGAC 
AAACATGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCCACC CTCTGGCAGT 
GCTGATGCCA GTCCCGCTGG GGACAGGGAT CGCCAGACTT CTCTGGAGAC CAGTATCCTC 
ACTCCCAGCC CTTGCAAAAT TCCACCTCAG AGGGGAGTGA GCTTTGGAAG TGGGCAGCTT 
CCCCCATATG ATCATCTCTT TGAGGTGGCC TTGCCAAAGA CTGCCTGTCA CTTTGTCAGC 
AAAAAGACTG AAGAGCTGTT GAAGAAAGCG AAAGGAAACC CTGAGGAAGA CTGTGTGCCC 
TCTACCTCCC CAATGGAAGT ACTGGACAGA CTGCTAGAGC AGGGAGCAGG GGCACACAGC 
AAGGAACTGA GCAGGTTGTC CTTGCCCAGC AAGTCTGTTG ACTGGACCCA CTTTGGAGGC 
TCTCCCCCCT CAGATGAGAT CCGGACCCTC CGAGACCAGT TGCTCTTACT GCACAATCAG 
CTGCTGTACG AACGCTTTAA GCGGCAGCAG CATGCACTGA GGAACAGAAG GCTGCTGCGC 
AAGGTGATCC GAGCTGCGGC CCTGGAGGAG CACAACGCAG CGATGAAAGA TCAGTTGAAG 
TTACAAGAGA AGGACATCCA GATGTGGAAG GTGAGTCTGC AGAAAGAACA AGCCCGATAC 
AGTCAGCTTC AGCAACAACG TGACACCATG GTGACCCAGC TGCATAGCCA AATCAGACAG 
CTGCAGCATG ACCGAGAAGA ATTCTACAAC CAGAGTCAGG AGTTACAGAC GAAGCTGGAG 
GACTGCAGAA GCATGATCGC GGAGCTTCGA GTGGAGCTGA AGAAGGCCAA CAGCAAGGTG 
TGCCACACCG AGCTGCTGCT CAGCCAGGTC TCTCAGAAGC TCTCCAATAG TGAGTCAGTG 
CAGCAGCAGA TGGAGTTCTT GAATAGGCAG CTTCTGGTGC TCGGGGAGGT CAATGAGCTG 
TACCTGGAGC AGCTGCAGAG CAAGCATCCT GACACCACCA AGGAAGTAGA AATGATGAAA 
ACTGCTTATC GGAAAGAACT AGAGAAAAAC AGAAGCCACC TTCTCCAGCA GAACCAGAGG 
CTGGATGCCT CACAGAGGCG AGTTTTAGAA CTGGAATCTC TTCTGGCCAA GAAAGACCAC 
CTTCTCCTAG AGCAGAAGAA GTACCTTGAG GATGTCAAGA GCCAGGCGAG TGGACAGCTG 
CTGGCTGCAG AGAGCAGGTA TGAGGCTCAG AGAAAGATCA CCCGGGTGTT GGAACTGGAG 
ATCCTAGACT TGTATGGTAG ATTGGAGAAA GATGGCCGCC TACAGAAACT AGAAGAGGAC 
AGAGCAGAGG CAGCAGAGGC AGCTGAAGAG AGGCTTGACT GTTGTACTGA TGGATGCTCA 
GATTCCTTGT TAGGACATAA TGAAGAGGCT GCTGGTCACA ATGGTGAGAC CAGGACCTCC 
AGACCTGGTG GCACCCGGGC CAGCTGTGGA GGTAGAGTCA CTGGAGGCAG CAGCAGCAGC 
AGCAGTGAGC TTTCCACTCC AGAGAAACCC CCGAACCAGA GGTTCAGCAG CCGGTGGGAG 
CCCACCATGG GTGAGCCCTC CAGCAGCATC CCCACCACTG TTGGCTCACT TCCCAGTTCC 
AAAAGCTTCT TGGGCATGAA GACCCGGGAG CTGTTCCGTA ATAAGAGTGA GAGCCAGTGT 
GATGAGGATG GCATGACCAT GAGTAGCTTT TCTGAGACCC TGAAGACAGA ACTGGGCAAA 
GACTCGGCGG GCATGGAAAA CAAGACTCCC CCAAGTCTAG ACGCCCCACA CCCATCTTCC 
CCAAGCTCAG ACAGTATGGG GCAGCTCCAT ATCATGGACT ACAATGAGAC TCATCACGAA 
CACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447505.1
CDS	187..3315
Translation	MDTDVVVLTTGVLVLITMLPMIPQSGKQHLLDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENVETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKTLETHDVVIECAKISLDPTEASYEDGDAVSHQLSACFPHRSADVTTSSYVDTQNSYGGATSTPSSTSRLMLFSTPGQLPQSLSSLSTRPLPEPLQASLWSPSAVCGMTTPPTSPGNVPADLSHPYSKAFGTTTGGKGTPSGTPATSPPPAPPCPQDDCAHGPASQASATPPRKEERADSSRPYLPRQQDVPSDRGLEDLPGSKGSVTLRNLPDFLGDLASEEDSIEKDKEEAAISKELSEITTAEADPVAPRGGFDSPFYRDSLSGSQRKTHSAASGTQGFSVNPEPLHSSLDKHGPDTPKQAFTPIDPPSGSADASPAGDRDRQTSLETSILTPSPCKIPPQRGVSFGSGQLPPYDHLFEVALPKTACHFVSKKTEELLKKAKGNPEEDCVPSTSPMEVLDRLLEQGAGAHSKELSRLSLPSKSVDWTHFGGSPPSDEIRTLRDQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRAAALEEHNAAMKDQLKLQEKDIQMWKVSLQKEQARYSQLQQQRDTMVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRSMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQSKHPDTTKEVEMMKTAYRKELEKNRSHLLQQNQRLDASQRRVLELESLLAKKDHLLLEQKKYLEDVKSQASGQLLAAESRYEAQRKITRVLELEILDLYGRLEKDGRLQKLEEDRAEAAEAAEERLDCCTDGCSDSLLGHNEEAAGHNGETRTSRPGGTRASCGGRVTGGSSSSSSELSTPEKPPNQRFSSRWEPTMGEPSSSIPTTVGSLPSSKSFLGMKTRELFRNKSESQCDEDGMTMSSFSETLKTELGKDSAGMENKTPPSLDAPHPSSPSSDSMGQLHIMDYNETHHEHS

Target ORF information:

RefSeq Version	XM_017592016.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tuberous sclerosis 1 (Tsc1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592016.1

ORF Insert Sequence:

ATGGACACTG ATGTTGTGGT TCTCACAACT GGTGTCTTGG TGTTGATCAC CATGCTACCG 
ATGATCCCGC AGTCAGGGAA GCAGCACCTT CTCGACTTCT TTGACATCTT TGGCCGTCTT 
TCATCATGGT GCCTGAAGAA ACCAGGCCAT GTGACAGAAG TCTACCTGGT CCACCTCCAT 
GCCAGTGTTT ATGCCCTCTT CCATCGCCTT TATGGAATGT ACCCTTGTAA CTTCGTCTCC 
TTCCTGCGCT CTCACTACAG CATGAAGGAA AACGTGGAGA CTTTTGAAGA AGTGGTCAAG 
CCAATGATGG AGCATGTGCG AATTCACCCG GAATTAGTGA CTGGATCCAA GGACCATGAA 
CTGGACCCTC GAAGGTGGAA GACATTAGAA ACTCATGATG TTGTAATAGA GTGTGCCAAA 
ATCTCTCTGG ACCCTACCGA AGCCTCATAT GAAGATGGCG ATGCTGTGTC TCACCAGCTC 
TCTGCCTGCT TCCCTCACCG TTCAGCTGAT GTCACCACCA GCTCTTACGT GGACACACAG 
AATAGCTATG GGGGCGCTAC TTCCACCCCT TCCTCCACCT CTCGGCTGAT GTTGTTCAGT 
ACCCCCGGGC AGCTACCTCA GAGTTTGAGT TCACTATCAA CACGGCCGTT ACCTGAGCCG 
CTGCAAGCTA GTCTTTGGAG CCCATCTGCA GTCTGTGGTA TGACCACTCC TCCTACGTCT 
CCTGGAAATG TCCCGGCTGA TTTGTCACAT CCATACAGTA AAGCCTTTGG TACTACCACT 
GGTGGAAAAG GAACTCCTTC AGGAACCCCA GCGACCTCTC CTCCCCCAGC CCCGCCTTGT 
CCCCAAGACG ACTGTGCGCA CGGTCCAGCC TCCCAGGCCT CAGCCACACC CCCCAGGAAG 
GAAGAAAGAG CAGATTCTTC AAGGCCTTAC CTACCCAGAC AGCAGGATGT TCCGAGTGAC 
CGCGGATTAG AGGATCTACC TGGAAGCAAA GGTTCCGTCA CTCTGAGGAA TCTACCAGAT 
TTCCTAGGTG ACCTGGCTTC AGAGGAAGAC AGTATAGAGA AAGATAAGGA AGAAGCTGCG 
ATATCTAAAG AGCTTTCTGA GATCACCACT GCAGAGGCGG ATCCTGTAGC TCCTCGAGGG 
GGCTTTGACT CTCCCTTCTA CCGAGACAGT CTCTCTGGTT CTCAGCGGAA GACTCACTCA 
GCAGCCTCTG GGACTCAGGG CTTCAGCGTG AACCCTGAGC CTTTGCACTC CTCCCTGGAC 
AAACATGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCCACC CTCTGGCAGT 
GCTGATGCCA GTCCCGCTGG GGACAGGGAT CGCCAGACTT CTCTGGAGAC CAGTATCCTC 
ACTCCCAGCC CTTGCAAAAT TCCACCTCAG AGGGGAGTGA GCTTTGGAAG TGGGCAGCTT 
CCCCCATATG ATCATCTCTT TGAGGTGGCC TTGCCAAAGA CTGCCTGTCA CTTTGTCAGC 
AAAAAGACTG AAGAGCTGTT GAAGAAAGCG AAAGGAAACC CTGAGGAAGA CTGTGTGCCC 
TCTACCTCCC CAATGGAAGT ACTGGACAGA CTGCTAGAGC AGGGAGCAGG GGCACACAGC 
AAGGAACTGA GCAGGTTGTC CTTGCCCAGC AAGTCTGTTG ACTGGACCCA CTTTGGAGGC 
TCTCCCCCCT CAGATGAGAT CCGGACCCTC CGAGACCAGT TGCTCTTACT GCACAATCAG 
CTGCTGTACG AACGCTTTAA GCGGCAGCAG CATGCACTGA GGAACAGAAG GCTGCTGCGC 
AAGGTGATCC GAGCTGCGGC CCTGGAGGAG CACAACGCAG CGATGAAAGA TCAGTTGAAG 
TTACAAGAGA AGGACATCCA GATGTGGAAG GTGAGTCTGC AGAAAGAACA AGCCCGATAC 
AGTCAGCTTC AGCAACAACG TGACACCATG GTGACCCAGC TGCATAGCCA AATCAGACAG 
CTGCAGCATG ACCGAGAAGA ATTCTACAAC CAGAGTCAGG AGTTACAGAC GAAGCTGGAG 
GACTGCAGAA GCATGATCGC GGAGCTTCGA GTGGAGCTGA AGAAGGCCAA CAGCAAGGTG 
TGCCACACCG AGCTGCTGCT CAGCCAGGTC TCTCAGAAGC TCTCCAATAG TGAGTCAGTG 
CAGCAGCAGA TGGAGTTCTT GAATAGGCAG CTTCTGGTGC TCGGGGAGGT CAATGAGCTG 
TACCTGGAGC AGCTGCAGAG CAAGCATCCT GACACCACCA AGGAAGTAGA AATGATGAAA 
ACTGCTTATC GGAAAGAACT AGAGAAAAAC AGAAGCCACC TTCTCCAGCA GAACCAGAGG 
CTGGATGCCT CACAGAGGCG AGTTTTAGAA CTGGAATCTC TTCTGGCCAA GAAAGACCAC 
CTTCTCCTAG AGCAGAAGAA GTACCTTGAG GATGTCAAGA GCCAGGCGAG TGGACAGCTG 
CTGGCTGCAG AGAGCAGGTA TGAGGCTCAG AGAAAGATCA CCCGGGTGTT GGAACTGGAG 
ATCCTAGACT TGTATGGTAG ATTGGAGAAA GATGGCCGCC TACAGAAACT AGAAGAGGAC 
AGAGCAGAGG CAGCAGAGGC AGCTGAAGAG AGGCTTGACT GTTGTACTGA TGGATGCTCA 
GATTCCTTGT TAGGACATAA TGAAGAGGCT GCTGGTCACA ATGGTGAGAC CAGGACCTCC 
AGACCTGGTG GCACCCGGGC CAGCTGTGGA GGTAGAGTCA CTGGAGGCAG CAGCAGCAGC 
AGCAGTGAGC TTTCCACTCC AGAGAAACCC CCGAACCAGA GGTTCAGCAG CCGGTGGGAG 
CCCACCATGG GTGAGCCCTC CAGCAGCATC CCCACCACTG TTGGCTCACT TCCCAGTTCC 
AAAAGCTTCT TGGGCATGAA GACCCGGGAG CTGTTCCGTA ATAAGAGTGA GAGCCAGTGT 
GATGAGGATG GCATGACCAT GAGTAGCTTT TCTGAGACCC TGAAGACAGA ACTGGGCAAA 
GACTCGGCGG GCATGGAAAA CAAGACTCCC CCAAGTCTAG ACGCCCCACA CCCATCTTCC 
CCAAGCTCAG ACAGTATGGG GCAGCTCCAT ATCATGGACT ACAATGAGAC TCATCACGAA 
CACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc1 ( NM_021854.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_068626.1 Rattus norvegicus TSC complex subunit 1 (Tsc1), mRNA.

Price & Availability↑

CloneID	ORa05290
Clone ID Related Accession (Same CDS sequence)	NM_021854.1
Accession Version	NM_021854.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	hamartin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB011821.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB011821.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCCAGC TAGCCAACAT TGGGGAGCTA CTCTCCATGC TGGACTCTTC CACACTGGGT 
GTGCGTGATG ACGTGACAAC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG 
CTTGTAAACA CGTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT CTTGCACATC 
CTGACCACCC TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAGTATGTA 
GGCAAAGCTG CCACCCGCTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTACT GAAATGTCTC 
AAGATGGACA CTGATGTTGT GGTTCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTA 
CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT 
CTTTCATCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTACCT GGTCCACCTC 
CATGCCAGTG TTTATGCCCT CTTCCATCGC CTTTATGGAA TGTACCCTTG TAACTTCGTC 
TCCTTCCTGC GCTCTCACTA CAGCATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCTAC CGAAGCCTCA TATGAAGATG GCGATGCTGT GTCTCACCAG 
CTCTCTGCCT GCTTCCCTCA CCGTTCAGCT GATGTCACCA CCAGCTCTTA CGTGGACACA 
CAGAATAGCT ATGGGGGCGC TACTTCCACC CCTTCCTCCA CCTCTCGGCT GATGTTGTTC 
AGTACCCCCG GGCAGCTACC TCAGAGTTTG AGTTCACTAT CAACACGGCC GTTACCTGAG 
CCGCTGCAAG CTAGTCTTTG GAGCCCATCT GCAGTCTGTG GTATGACCAC TCCTCCTACG 
TCTCCTGGAA ATGTCCCGGC TGATTTGTCA CATCCATACA GTAAAGCCTT TGGTACTACC 
ACTGGTGGAA AAGGAACTCC TTCAGGAACC CCAGCGACCT CTCCTCCCCC AGCCCCGCCT 
TGTCCCCAAG ACGACTGTGC GCACGGTCCA GCCTCCCAGG CCTCAGCCAC ACCCCCCAGG 
AAGGAAGAAA GAGCAGATTC TTCAAGGCCT TACCTACCCA GACAGCAGGA TGTTCCGAGT 
GACCGCGGAT TAGAGGATCT ACCTGGAAGC AAAGGTTCCG TCACTCTGAG GAATCTACCA 
GATTTCCTAG GTGACCTGGC TTCAGAGGAA GACAGTATAG AGAAAGATAA GGAAGAAGCT 
GCGATATCTA AAGAGCTTTC TGAGATCACC ACTGCAGAGG CGGATCCTGT AGCTCCTCGA 
GGGGGCTTTG ACTCTCCCTT CTACCGAGAC AGTCTCTCTG GTTCTCAGCG GAAGACTCAC 
TCAGCAGCCT CTGGGACTCA GGGCTTCAGC GTGAACCCTG AGCCTTTGCA CTCCTCCCTG 
GACAAACATG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCC ACCCTCTGGC 
AGTGCTGATG CCAGTCCCGC TGGGGACAGG GATCGCCAGA CTTCTCTGGA GACCAGTATC 
CTCACTCCCA GCCCTTGCAA AATTCCACCT CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG 
CTTCCCCCAT ATGATCATCT CTTTGAGGTG GCCTTGCCAA AGACTGCCTG TCACTTTGTC 
AGCAAAAAGA CTGAAGAGCT GTTGAAGAAA GCGAAAGGAA ACCCTGAGGA AGACTGTGTG 
CCCTCTACCT CCCCAATGGA AGTACTGGAC AGACTGCTAG AGCAGGGAGC AGGGGCACAC 
AGCAAGGAAC TGAGCAGGTT GTCCTTGCCC AGCAAGTCTG TTGACTGGAC CCACTTTGGA 
GGCTCTCCCC CCTCAGATGA GATCCGGACC CTCCGAGACC AGTTGCTCTT ACTGCACAAT 
CAGCTGCTGT ACGAACGCTT TAAGCGGCAG CAGCATGCAC TGAGGAACAG AAGGCTGCTG 
CGCAAGGTGA TCCGAGCTGC GGCCCTGGAG GAGCACAACG CAGCGATGAA AGATCAGTTG 
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA 
TACAGTCAGC TTCAGCAACA ACGTGACACC ATGGTGACCC AGCTGCATAG CCAAATCAGA 
CAGCTGCAGC ATGACCGAGA AGAATTCTAC AACCAGAGTC AGGAGTTACA GACGAAGCTG 
GAGGACTGCA GAAGCATGAT CGCGGAGCTT CGAGTGGAGC TGAAGAAGGC CAACAGCAAG 
GTGTGCCACA CCGAGCTGCT GCTCAGCCAG GTCTCTCAGA AGCTCTCCAA TAGTGAGTCA 
GTGCAGCAGC AGATGGAGTT CTTGAATAGG CAGCTCCTGG TGCTCGGGGA GGTCAATGAG 
CTGTACCTGG AGCAGCTGCA GAGCAAGCAT CCTGACACCA CCAAGGAAGT AGAAATGATG 
AAAACTGCTT ATCGGAAAGA ACTAGAGAAA AACAGAAGCC ACCTTCTCCA GCAGAACCAG 
AGGCTGGATG CCTCACAGAG GCGAGTTTTA GAACTGGAAT CTCTTCTGGC CAAGAAAGAC 
CACCTTCTCC TAGAGCAGAA GAAGTACCTT GAGGATGTCA AGAGCCAGGC GAGTGGACAG 
CTGCTGGCTG CAGAGAGCAG GTATGAGGCT CAGAGAAAGA TCACCCGGGT GTTGGAACTG 
GAGATCCTAG ACTTGTATGG TAGATTGGAG AAAGATGGCC GCCTACAGAA ACTAGAAGAG 
GACAGAGCAG AGGCAGCAGA GGCAGCTGAA GAGAGGCTTG ACTGTTGTAC TGATGGATGC 
TCAGATTCCT TGTTAGGACA TAATGAAGAG GCTGCTGGTC ACAATGGTGA GACCAGGACC 
TCCAGACCTG GTGGCACCCG GGCCAGCTGT GGAGGTAGAG TCACTGGAGG CAGCAGCAGC 
AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA CCCCCGAACC AGAGGTTCAG CAGCCGGTGG 
GAGCCCACCA TGGGTGAGCC CTCCAGCAGC ATCCCCACCA CTGTTGGCTC ACTTCCCAGT 
TCCAAAAGCT TCTTGGGCAT GAAGACCCGG GAGCTGTTCC GTAATAAGAG TGAGAGCCAG 
TGTGATGAGG ATGGCATGAC CATGAGTAGC TTTTCTGAGA CCCTGAAGAC AGAACTGGGC 
AAAGACTCGG CGGGCATGGA AAACAAGACT CCCCCAAGTC TAGACGCCCC ACACCCATCT 
TCCCCAAGCT CAGACAGTAT GGGGCAGCTC CATATCATGG ACTACAATGA GACTCATCAC 
GAACACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068626.1
CDS	1..3492
Translation	MAQLANIGELLSMLDSSTLGVRDDVTTIFKESLNSERGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAATRLSILSLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVVVLTTGVLVLITMLPMIPQSGKQHLLDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENVETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKTLETHDVVIECAKISLDPTEASYEDGDAVSHQLSACFPHRSADVTTSSYVDTQNSYGGATSTPSSTSRLMLFSTPGQLPQSLSSLSTRPLPEPLQASLWSPSAVCGMTTPPTSPGNVPADLSHPYSKAFGTTTGGKGTPSGTPATSPPPAPPCPQDDCAHGPASQASATPPRKEERADSSRPYLPRQQDVPSDRGLEDLPGSKGSVTLRNLPDFLGDLASEEDSIEKDKEEAAISKELSEITTAEADPVAPRGGFDSPFYRDSLSGSQRKTHSAASGTQGFSVNPEPLHSSLDKHGPDTPKQAFTPIDPPSGSADASPAGDRDRQTSLETSILTPSPCKIPPQRGVSFGSGQLPPYDHLFEVALPKTACHFVSKKTEELLKKAKGNPEEDCVPSTSPMEVLDRLLEQGAGAHSKELSRLSLPSKSVDWTHFGGSPPSDEIRTLRDQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRAAALEEHNAAMKDQLKLQEKDIQMWKVSLQKEQARYSQLQQQRDTMVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRSMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQSKHPDTTKEVEMMKTAYRKELEKNRSHLLQQNQRLDASQRRVLELESLLAKKDHLLLEQKKYLEDVKSQASGQLLAAESRYEAQRKITRVLELEILDLYGRLEKDGRLQKLEEDRAEAAEAAEERLDCCTDGCSDSLLGHNEEAAGHNGETRTSRPGGTRASCGGRVTGGSSSSSSELSTPEKPPNQRFSSRWEPTMGEPSSSIPTTVGSLPSSKSFLGMKTRELFRNKSESQCDEDGMTMSSFSETLKTELGKDSAGMENKTPPSLDAPHPSSPSSDSMGQLHIMDYNETHHEHS

Target ORF information:

RefSeq Version	NM_021854.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus TSC complex subunit 1 (Tsc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021854.1

ORF Insert Sequence:

ATGGCCCAGC TAGCCAACAT TGGGGAGCTA CTCTCCATGC TGGACTCTTC CACACTGGGT 
GTGCGTGATG ACGTGACAAC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG 
CTTGTAAACA CGTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT CTTGCACATC 
CTGACCACCC TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAGTATGTA 
GGCAAAGCTG CCACCCGCTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTACT GAAATGTCTC 
AAGATGGACA CTGATGTTGT GGTTCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTA 
CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT 
CTTTCATCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTACCT GGTCCACCTC 
CATGCCAGTG TTTATGCCCT CTTCCATCGC CTTTATGGAA TGTACCCTTG TAACTTCGTC 
TCCTTCCTGC GCTCTCACTA CAGCATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCTAC CGAAGCCTCA TATGAAGATG GCGATGCTGT GTCTCACCAG 
CTCTCTGCCT GCTTCCCTCA CCGTTCAGCT GATGTCACCA CCAGCTCTTA CGTGGACACA 
CAGAATAGCT ATGGGGGCGC TACTTCCACC CCTTCCTCCA CCTCTCGGCT GATGTTGTTC 
AGTACCCCCG GGCAGCTACC TCAGAGTTTG AGTTCACTAT CAACACGGCC GTTACCTGAG 
CCGCTGCAAG CTAGTCTTTG GAGCCCATCT GCAGTCTGTG GTATGACCAC TCCTCCTACG 
TCTCCTGGAA ATGTCCCGGC TGATTTGTCA CATCCATACA GTAAAGCCTT TGGTACTACC 
ACTGGTGGAA AAGGAACTCC TTCAGGAACC CCAGCGACCT CTCCTCCCCC AGCCCCGCCT 
TGTCCCCAAG ACGACTGTGC GCACGGTCCA GCCTCCCAGG CCTCAGCCAC ACCCCCCAGG 
AAGGAAGAAA GAGCAGATTC TTCAAGGCCT TACCTACCCA GACAGCAGGA TGTTCCGAGT 
GACCGCGGAT TAGAGGATCT ACCTGGAAGC AAAGGTTCCG TCACTCTGAG GAATCTACCA 
GATTTCCTAG GTGACCTGGC TTCAGAGGAA GACAGTATAG AGAAAGATAA GGAAGAAGCT 
GCGATATCTA AAGAGCTTTC TGAGATCACC ACTGCAGAGG CGGATCCTGT AGCTCCTCGA 
GGGGGCTTTG ACTCTCCCTT CTACCGAGAC AGTCTCTCTG GTTCTCAGCG GAAGACTCAC 
TCAGCAGCCT CTGGGACTCA GGGCTTCAGC GTGAACCCTG AGCCTTTGCA CTCCTCCCTG 
GACAAACATG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCC ACCCTCTGGC 
AGTGCTGATG CCAGTCCCGC TGGGGACAGG GATCGCCAGA CTTCTCTGGA GACCAGTATC 
CTCACTCCCA GCCCTTGCAA AATTCCACCT CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG 
CTTCCCCCAT ATGATCATCT CTTTGAGGTG GCCTTGCCAA AGACTGCCTG TCACTTTGTC 
AGCAAAAAGA CTGAAGAGCT GTTGAAGAAA GCGAAAGGAA ACCCTGAGGA AGACTGTGTG 
CCCTCTACCT CCCCAATGGA AGTACTGGAC AGACTGCTAG AGCAGGGAGC AGGGGCACAC 
AGCAAGGAAC TGAGCAGGTT GTCCTTGCCC AGCAAGTCTG TTGACTGGAC CCACTTTGGA 
GGCTCTCCCC CCTCAGATGA GATCCGGACC CTCCGAGACC AGTTGCTCTT ACTGCACAAT 
CAGCTGCTGT ACGAACGCTT TAAGCGGCAG CAGCATGCAC TGAGGAACAG AAGGCTGCTG 
CGCAAGGTGA TCCGAGCTGC GGCCCTGGAG GAGCACAACG CAGCGATGAA AGATCAGTTG 
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA 
TACAGTCAGC TTCAGCAACA ACGTGACACC ATGGTGACCC AGCTGCATAG CCAAATCAGA 
CAGCTGCAGC ATGACCGAGA AGAATTCTAC AACCAGAGTC AGGAGTTACA GACGAAGCTG 
GAGGACTGCA GAAGCATGAT CGCGGAGCTT CGAGTGGAGC TGAAGAAGGC CAACAGCAAG 
GTGTGCCACA CCGAGCTGCT GCTCAGCCAG GTCTCTCAGA AGCTCTCCAA TAGTGAGTCA 
GTGCAGCAGC AGATGGAGTT CTTGAATAGG CAGCTCCTGG TGCTCGGGGA GGTCAATGAG 
CTGTACCTGG AGCAGCTGCA GAGCAAGCAT CCTGACACCA CCAAGGAAGT AGAAATGATG 
AAAACTGCTT ATCGGAAAGA ACTAGAGAAA AACAGAAGCC ACCTTCTCCA GCAGAACCAG 
AGGCTGGATG CCTCACAGAG GCGAGTTTTA GAACTGGAAT CTCTTCTGGC CAAGAAAGAC 
CACCTTCTCC TAGAGCAGAA GAAGTACCTT GAGGATGTCA AGAGCCAGGC GAGTGGACAG 
CTGCTGGCTG CAGAGAGCAG GTATGAGGCT CAGAGAAAGA TCACCCGGGT GTTGGAACTG 
GAGATCCTAG ACTTGTATGG TAGATTGGAG AAAGATGGCC GCCTACAGAA ACTAGAAGAG 
GACAGAGCAG AGGCAGCAGA GGCAGCTGAA GAGAGGCTTG ACTGTTGTAC TGATGGATGC 
TCAGATTCCT TGTTAGGACA TAATGAAGAG GCTGCTGGTC ACAATGGTGA GACCAGGACC 
TCCAGACCTG GTGGCACCCG GGCCAGCTGT GGAGGTAGAG TCACTGGAGG CAGCAGCAGC 
AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA CCCCCGAACC AGAGGTTCAG CAGCCGGTGG 
GAGCCCACCA TGGGTGAGCC CTCCAGCAGC ATCCCCACCA CTGTTGGCTC ACTTCCCAGT 
TCCAAAAGCT TCTTGGGCAT GAAGACCCGG GAGCTGTTCC GTAATAAGAG TGAGAGCCAG 
TGTGATGAGG ATGGCATGAC CATGAGTAGC TTTTCTGAGA CCCTGAAGAC AGAACTGGGC 
AAAGACTCGG CGGGCATGGA AAACAAGACT CCCCCAAGTC TAGACGCCCC ACACCCATCT 
TCCCCAAGCT CAGACAGTAT GGGGCAGCTC CATATCATGG ACTACAATGA GACTCATCAC 
GAACACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc1 ( XM_006233844.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233906.1 Rattus norvegicus tuberous sclerosis 1 (Tsc1), transcript variant X2, mRNA.

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CloneID	ORa25661
Clone ID Related Accession (Same CDS sequence)	XM_006233844.2 , XM_006233846.2 , XM_006233845.2 , XM_006233847.3 , XM_006233843.2
Accession Version	XM_006233844.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006233844.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC TAGCCAACAT TGGGGAGCTA CTCTCCATGC TGGACTCTTC CACACTGGGT 
GTGCGTGATG ACGTGACAAC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG 
CTTGTAAACA CGTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT CTTGCACATC 
CTGACCACCC TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAGTATGTA 
GGCAAAGCTG CCACCCGCTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTACT GAAATGTCTC 
AAGATGGACA CTGATGTTGT GGTTCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTA 
CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT 
CTTTCATCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTACCT GGTCCACCTC 
CATGCCAGTG TTTATGCCCT CTTCCATCGC CTTTATGGAA TGTACCCTTG TAACTTCGTC 
TCCTTCCTGC GCTCTCACTA CAGCATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCTAC CGAAGCCTCA TATGAAGATG GCGATGCTGT GTCTCACCAG 
CTCTCTGCCT GCTTCCCTCA CCGTTCAGCT GATGTCACCA CCAGCTCTTA CGTGGACACA 
CAGAATAGCT ATGGGGGCGC TACTTCCACC CCTTCCTCCA CCTCTCGGCT GATGTTGTTC 
AGTACCCCCG GGCAGCTACC TCAGAGTTTG AGTTCACTAT CAACACGGCC GTTACCTGAG 
CCGCTGCAAG CTAGTCTTTG GAGCCCATCT GCAGTCTGTG GTATGACCAC TCCTCCTACG 
TCTCCTGGAA ATGTCCCGGC TGATTTGTCA CATCCATACA GTAAAGCCTT TGGTACTACC 
ACTGGTGGAA AAGGAACTCC TTCAGGAACC CCAGCGACCT CTCCTCCCCC AGCCCCGCCT 
TGTCCCCAAG ACGACTGTGC GCACGGTCCA GCCTCCCAGG CCTCAGCCAC ACCCCCCAGG 
AAGGAAGAAA GAGCAGATTC TTCAAGGCCT TACCTACCCA GACAGCAGGA TGTTCCGAGT 
GACCGCGGAT TAGAGGATCT ACCTGGAAGC AAAGGTTCCG TCACTCTGAG GAATCTACCA 
GATTTCCTAG GTGACCTGGC TTCAGAGGAA GACAGTATAG AGAAAGATAA GGAAGAAGCT 
GCGATATCTA AAGAGCTTTC TGAGATCACC ACTGCAGAGG CGGATCCTGT AGCTCCTCGA 
GGGGGCTTTG ACTCTCCCTT CTACCGAGAC AGTCTCTCTG GTTCTCAGCG GAAGACTCAC 
TCAGCAGCCT CTGGGACTCA GGGCTTCAGC GTGAACCCTG AGCCTTTGCA CTCCTCCCTG 
GACAAACATG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCC ACCCTCTGGC 
AGTGCTGATG CCAGTCCCGC TGGGGACAGG GATCGCCAGA CTTCTCTGGA GACCAGTATC 
CTCACTCCCA GCCCTTGCAA AATTCCACCT CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG 
CTTCCCCCAT ATGATCATCT CTTTGAGGTG GCCTTGCCAA AGACTGCCTG TCACTTTGTC 
AGCAAAAAGA CTGAAGAGCT GTTGAAGAAA GCGAAAGGAA ACCCTGAGGA AGACTGTGTG 
CCCTCTACCT CCCCAATGGA AGTACTGGAC AGACTGCTAG AGCAGGGAGC AGGGGCACAC 
AGCAAGGAAC TGAGCAGGTT GTCCTTGCCC AGCAAGTCTG TTGACTGGAC CCACTTTGGA 
GGCTCTCCCC CCTCAGATGA GATCCGGACC CTCCGAGACC AGTTGCTCTT ACTGCACAAT 
CAGCTGCTGT ACGAACGCTT TAAGCGGCAG CAGCATGCAC TGAGGAACAG AAGGCTGCTG 
CGCAAGGTGA TCCGAGCTGC GGCCCTGGAG GAGCACAACG CAGCGATGAA AGATCAGTTG 
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA 
TACAGTCAGC TTCAGCAACA ACGTGACACC ATGGTGACCC AGCTGCATAG CCAAATCAGA 
CAGCTGCAGC ATGACCGAGA AGAATTCTAC AACCAGAGTC AGGAGTTACA GACGAAGCTG 
GAGGACTGCA GAAGCATGAT CGCGGAGCTT CGAGTGGAGC TGAAGAAGGC CAACAGCAAG 
GTGTGCCACA CCGAGCTGCT GCTCAGCCAG GTCTCTCAGA AGCTCTCCAA TAGTGAGTCA 
GTGCAGCAGC AGATGGAGTT CTTGAATAGG CAGCTTCTGG TGCTCGGGGA GGTCAATGAG 
CTGTACCTGG AGCAGCTGCA GAGCAAGCAT CCTGACACCA CCAAGGAAGT AGAAATGATG 
AAAACTGCTT ATCGGAAAGA ACTAGAGAAA AACAGAAGCC ACCTTCTCCA GCAGAACCAG 
AGGCTGGATG CCTCACAGAG GCGAGTTTTA GAACTGGAAT CTCTTCTGGC CAAGAAAGAC 
CACCTTCTCC TAGAGCAGAA GAAGTACCTT GAGGATGTCA AGAGCCAGGC GAGTGGACAG 
CTGCTGGCTG CAGAGAGCAG GTATGAGGCT CAGAGAAAGA TCACCCGGGT GTTGGAACTG 
GAGATCCTAG ACTTGTATGG TAGATTGGAG AAAGATGGCC GCCTACAGAA ACTAGAAGAG 
GACAGAGCAG AGGCAGCAGA GGCAGCTGAA GAGAGGCTTG ACTGTTGTAC TGATGGATGC 
TCAGATTCCT TGTTAGGACA TAATGAAGAG GCTGCTGGTC ACAATGGTGA GACCAGGACC 
TCCAGACCTG GTGGCACCCG GGCCAGCTGT GGAGGTAGAG TCACTGGAGG CAGCAGCAGC 
AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA CCCCCGAACC AGAGGTTCAG CAGCCGGTGG 
GAGCCCACCA TGGGTGAGCC CTCCAGCAGC ATCCCCACCA CTGTTGGCTC ACTTCCCAGT 
TCCAAAAGCT TCTTGGGCAT GAAGACCCGG GAGCTGTTCC GTAATAAGAG TGAGAGCCAG 
TGTGATGAGG ATGGCATGAC CATGAGTAGC TTTTCTGAGA CCCTGAAGAC AGAACTGGGC 
AAAGACTCGG CGGGCATGGA AAACAAGACT CCCCCAAGTC TAGACGCCCC ACACCCATCT 
TCCCCAAGCT CAGACAGTAT GGGGCAGCTC CATATCATGG ACTACAATGA GACTCATCAC 
GAACACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233906.1
CDS	426..3917
Translation	MAQLANIGELLSMLDSSTLGVRDDVTTIFKESLNSERGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAATRLSILSLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVVVLTTGVLVLITMLPMIPQSGKQHLLDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENVETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKTLETHDVVIECAKISLDPTEASYEDGDAVSHQLSACFPHRSADVTTSSYVDTQNSYGGATSTPSSTSRLMLFSTPGQLPQSLSSLSTRPLPEPLQASLWSPSAVCGMTTPPTSPGNVPADLSHPYSKAFGTTTGGKGTPSGTPATSPPPAPPCPQDDCAHGPASQASATPPRKEERADSSRPYLPRQQDVPSDRGLEDLPGSKGSVTLRNLPDFLGDLASEEDSIEKDKEEAAISKELSEITTAEADPVAPRGGFDSPFYRDSLSGSQRKTHSAASGTQGFSVNPEPLHSSLDKHGPDTPKQAFTPIDPPSGSADASPAGDRDRQTSLETSILTPSPCKIPPQRGVSFGSGQLPPYDHLFEVALPKTACHFVSKKTEELLKKAKGNPEEDCVPSTSPMEVLDRLLEQGAGAHSKELSRLSLPSKSVDWTHFGGSPPSDEIRTLRDQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRAAALEEHNAAMKDQLKLQEKDIQMWKVSLQKEQARYSQLQQQRDTMVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRSMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQSKHPDTTKEVEMMKTAYRKELEKNRSHLLQQNQRLDASQRRVLELESLLAKKDHLLLEQKKYLEDVKSQASGQLLAAESRYEAQRKITRVLELEILDLYGRLEKDGRLQKLEEDRAEAAEAAEERLDCCTDGCSDSLLGHNEEAAGHNGETRTSRPGGTRASCGGRVTGGSSSSSSELSTPEKPPNQRFSSRWEPTMGEPSSSIPTTVGSLPSSKSFLGMKTRELFRNKSESQCDEDGMTMSSFSETLKTELGKDSAGMENKTPPSLDAPHPSSPSSDSMGQLHIMDYNETHHEHS

Target ORF information:

RefSeq Version	XM_006233844.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tuberous sclerosis 1 (Tsc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233844.2

ORF Insert Sequence:

ATGGCCCAGC TAGCCAACAT TGGGGAGCTA CTCTCCATGC TGGACTCTTC CACACTGGGT 
GTGCGTGATG ACGTGACAAC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG 
CTTGTAAACA CGTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT CTTGCACATC 
CTGACCACCC TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAGTATGTA 
GGCAAAGCTG CCACCCGCTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTACT GAAATGTCTC 
AAGATGGACA CTGATGTTGT GGTTCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTA 
CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT 
CTTTCATCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTACCT GGTCCACCTC 
CATGCCAGTG TTTATGCCCT CTTCCATCGC CTTTATGGAA TGTACCCTTG TAACTTCGTC 
TCCTTCCTGC GCTCTCACTA CAGCATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCTAC CGAAGCCTCA TATGAAGATG GCGATGCTGT GTCTCACCAG 
CTCTCTGCCT GCTTCCCTCA CCGTTCAGCT GATGTCACCA CCAGCTCTTA CGTGGACACA 
CAGAATAGCT ATGGGGGCGC TACTTCCACC CCTTCCTCCA CCTCTCGGCT GATGTTGTTC 
AGTACCCCCG GGCAGCTACC TCAGAGTTTG AGTTCACTAT CAACACGGCC GTTACCTGAG 
CCGCTGCAAG CTAGTCTTTG GAGCCCATCT GCAGTCTGTG GTATGACCAC TCCTCCTACG 
TCTCCTGGAA ATGTCCCGGC TGATTTGTCA CATCCATACA GTAAAGCCTT TGGTACTACC 
ACTGGTGGAA AAGGAACTCC TTCAGGAACC CCAGCGACCT CTCCTCCCCC AGCCCCGCCT 
TGTCCCCAAG ACGACTGTGC GCACGGTCCA GCCTCCCAGG CCTCAGCCAC ACCCCCCAGG 
AAGGAAGAAA GAGCAGATTC TTCAAGGCCT TACCTACCCA GACAGCAGGA TGTTCCGAGT 
GACCGCGGAT TAGAGGATCT ACCTGGAAGC AAAGGTTCCG TCACTCTGAG GAATCTACCA 
GATTTCCTAG GTGACCTGGC TTCAGAGGAA GACAGTATAG AGAAAGATAA GGAAGAAGCT 
GCGATATCTA AAGAGCTTTC TGAGATCACC ACTGCAGAGG CGGATCCTGT AGCTCCTCGA 
GGGGGCTTTG ACTCTCCCTT CTACCGAGAC AGTCTCTCTG GTTCTCAGCG GAAGACTCAC 
TCAGCAGCCT CTGGGACTCA GGGCTTCAGC GTGAACCCTG AGCCTTTGCA CTCCTCCCTG 
GACAAACATG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCC ACCCTCTGGC 
AGTGCTGATG CCAGTCCCGC TGGGGACAGG GATCGCCAGA CTTCTCTGGA GACCAGTATC 
CTCACTCCCA GCCCTTGCAA AATTCCACCT CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG 
CTTCCCCCAT ATGATCATCT CTTTGAGGTG GCCTTGCCAA AGACTGCCTG TCACTTTGTC 
AGCAAAAAGA CTGAAGAGCT GTTGAAGAAA GCGAAAGGAA ACCCTGAGGA AGACTGTGTG 
CCCTCTACCT CCCCAATGGA AGTACTGGAC AGACTGCTAG AGCAGGGAGC AGGGGCACAC 
AGCAAGGAAC TGAGCAGGTT GTCCTTGCCC AGCAAGTCTG TTGACTGGAC CCACTTTGGA 
GGCTCTCCCC CCTCAGATGA GATCCGGACC CTCCGAGACC AGTTGCTCTT ACTGCACAAT 
CAGCTGCTGT ACGAACGCTT TAAGCGGCAG CAGCATGCAC TGAGGAACAG AAGGCTGCTG 
CGCAAGGTGA TCCGAGCTGC GGCCCTGGAG GAGCACAACG CAGCGATGAA AGATCAGTTG 
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA 
TACAGTCAGC TTCAGCAACA ACGTGACACC ATGGTGACCC AGCTGCATAG CCAAATCAGA 
CAGCTGCAGC ATGACCGAGA AGAATTCTAC AACCAGAGTC AGGAGTTACA GACGAAGCTG 
GAGGACTGCA GAAGCATGAT CGCGGAGCTT CGAGTGGAGC TGAAGAAGGC CAACAGCAAG 
GTGTGCCACA CCGAGCTGCT GCTCAGCCAG GTCTCTCAGA AGCTCTCCAA TAGTGAGTCA 
GTGCAGCAGC AGATGGAGTT CTTGAATAGG CAGCTTCTGG TGCTCGGGGA GGTCAATGAG 
CTGTACCTGG AGCAGCTGCA GAGCAAGCAT CCTGACACCA CCAAGGAAGT AGAAATGATG 
AAAACTGCTT ATCGGAAAGA ACTAGAGAAA AACAGAAGCC ACCTTCTCCA GCAGAACCAG 
AGGCTGGATG CCTCACAGAG GCGAGTTTTA GAACTGGAAT CTCTTCTGGC CAAGAAAGAC 
CACCTTCTCC TAGAGCAGAA GAAGTACCTT GAGGATGTCA AGAGCCAGGC GAGTGGACAG 
CTGCTGGCTG CAGAGAGCAG GTATGAGGCT CAGAGAAAGA TCACCCGGGT GTTGGAACTG 
GAGATCCTAG ACTTGTATGG TAGATTGGAG AAAGATGGCC GCCTACAGAA ACTAGAAGAG 
GACAGAGCAG AGGCAGCAGA GGCAGCTGAA GAGAGGCTTG ACTGTTGTAC TGATGGATGC 
TCAGATTCCT TGTTAGGACA TAATGAAGAG GCTGCTGGTC ACAATGGTGA GACCAGGACC 
TCCAGACCTG GTGGCACCCG GGCCAGCTGT GGAGGTAGAG TCACTGGAGG CAGCAGCAGC 
AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA CCCCCGAACC AGAGGTTCAG CAGCCGGTGG 
GAGCCCACCA TGGGTGAGCC CTCCAGCAGC ATCCCCACCA CTGTTGGCTC ACTTCCCAGT 
TCCAAAAGCT TCTTGGGCAT GAAGACCCGG GAGCTGTTCC GTAATAAGAG TGAGAGCCAG 
TGTGATGAGG ATGGCATGAC CATGAGTAGC TTTTCTGAGA CCCTGAAGAC AGAACTGGGC 
AAAGACTCGG CGGGCATGGA AAACAAGACT CCCCCAAGTC TAGACGCCCC ACACCCATCT 
TCCCCAAGCT CAGACAGTAT GGGGCAGCTC CATATCATGG ACTACAATGA GACTCATCAC 
GAACACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc1 ( XM_006233846.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233908.1 Rattus norvegicus tuberous sclerosis 1 (Tsc1), transcript variant X3, mRNA.

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CloneID	ORa25661
Clone ID Related Accession (Same CDS sequence)	XM_006233844.2 , XM_006233846.2 , XM_006233845.2 , XM_006233847.3 , XM_006233843.2
Accession Version	XM_006233846.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006233846.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC TAGCCAACAT TGGGGAGCTA CTCTCCATGC TGGACTCTTC CACACTGGGT 
GTGCGTGATG ACGTGACAAC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG 
CTTGTAAACA CGTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT CTTGCACATC 
CTGACCACCC TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAGTATGTA 
GGCAAAGCTG CCACCCGCTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTACT GAAATGTCTC 
AAGATGGACA CTGATGTTGT GGTTCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTA 
CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT 
CTTTCATCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTACCT GGTCCACCTC 
CATGCCAGTG TTTATGCCCT CTTCCATCGC CTTTATGGAA TGTACCCTTG TAACTTCGTC 
TCCTTCCTGC GCTCTCACTA CAGCATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCTAC CGAAGCCTCA TATGAAGATG GCGATGCTGT GTCTCACCAG 
CTCTCTGCCT GCTTCCCTCA CCGTTCAGCT GATGTCACCA CCAGCTCTTA CGTGGACACA 
CAGAATAGCT ATGGGGGCGC TACTTCCACC CCTTCCTCCA CCTCTCGGCT GATGTTGTTC 
AGTACCCCCG GGCAGCTACC TCAGAGTTTG AGTTCACTAT CAACACGGCC GTTACCTGAG 
CCGCTGCAAG CTAGTCTTTG GAGCCCATCT GCAGTCTGTG GTATGACCAC TCCTCCTACG 
TCTCCTGGAA ATGTCCCGGC TGATTTGTCA CATCCATACA GTAAAGCCTT TGGTACTACC 
ACTGGTGGAA AAGGAACTCC TTCAGGAACC CCAGCGACCT CTCCTCCCCC AGCCCCGCCT 
TGTCCCCAAG ACGACTGTGC GCACGGTCCA GCCTCCCAGG CCTCAGCCAC ACCCCCCAGG 
AAGGAAGAAA GAGCAGATTC TTCAAGGCCT TACCTACCCA GACAGCAGGA TGTTCCGAGT 
GACCGCGGAT TAGAGGATCT ACCTGGAAGC AAAGGTTCCG TCACTCTGAG GAATCTACCA 
GATTTCCTAG GTGACCTGGC TTCAGAGGAA GACAGTATAG AGAAAGATAA GGAAGAAGCT 
GCGATATCTA AAGAGCTTTC TGAGATCACC ACTGCAGAGG CGGATCCTGT AGCTCCTCGA 
GGGGGCTTTG ACTCTCCCTT CTACCGAGAC AGTCTCTCTG GTTCTCAGCG GAAGACTCAC 
TCAGCAGCCT CTGGGACTCA GGGCTTCAGC GTGAACCCTG AGCCTTTGCA CTCCTCCCTG 
GACAAACATG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCC ACCCTCTGGC 
AGTGCTGATG CCAGTCCCGC TGGGGACAGG GATCGCCAGA CTTCTCTGGA GACCAGTATC 
CTCACTCCCA GCCCTTGCAA AATTCCACCT CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG 
CTTCCCCCAT ATGATCATCT CTTTGAGGTG GCCTTGCCAA AGACTGCCTG TCACTTTGTC 
AGCAAAAAGA CTGAAGAGCT GTTGAAGAAA GCGAAAGGAA ACCCTGAGGA AGACTGTGTG 
CCCTCTACCT CCCCAATGGA AGTACTGGAC AGACTGCTAG AGCAGGGAGC AGGGGCACAC 
AGCAAGGAAC TGAGCAGGTT GTCCTTGCCC AGCAAGTCTG TTGACTGGAC CCACTTTGGA 
GGCTCTCCCC CCTCAGATGA GATCCGGACC CTCCGAGACC AGTTGCTCTT ACTGCACAAT 
CAGCTGCTGT ACGAACGCTT TAAGCGGCAG CAGCATGCAC TGAGGAACAG AAGGCTGCTG 
CGCAAGGTGA TCCGAGCTGC GGCCCTGGAG GAGCACAACG CAGCGATGAA AGATCAGTTG 
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA 
TACAGTCAGC TTCAGCAACA ACGTGACACC ATGGTGACCC AGCTGCATAG CCAAATCAGA 
CAGCTGCAGC ATGACCGAGA AGAATTCTAC AACCAGAGTC AGGAGTTACA GACGAAGCTG 
GAGGACTGCA GAAGCATGAT CGCGGAGCTT CGAGTGGAGC TGAAGAAGGC CAACAGCAAG 
GTGTGCCACA CCGAGCTGCT GCTCAGCCAG GTCTCTCAGA AGCTCTCCAA TAGTGAGTCA 
GTGCAGCAGC AGATGGAGTT CTTGAATAGG CAGCTTCTGG TGCTCGGGGA GGTCAATGAG 
CTGTACCTGG AGCAGCTGCA GAGCAAGCAT CCTGACACCA CCAAGGAAGT AGAAATGATG 
AAAACTGCTT ATCGGAAAGA ACTAGAGAAA AACAGAAGCC ACCTTCTCCA GCAGAACCAG 
AGGCTGGATG CCTCACAGAG GCGAGTTTTA GAACTGGAAT CTCTTCTGGC CAAGAAAGAC 
CACCTTCTCC TAGAGCAGAA GAAGTACCTT GAGGATGTCA AGAGCCAGGC GAGTGGACAG 
CTGCTGGCTG CAGAGAGCAG GTATGAGGCT CAGAGAAAGA TCACCCGGGT GTTGGAACTG 
GAGATCCTAG ACTTGTATGG TAGATTGGAG AAAGATGGCC GCCTACAGAA ACTAGAAGAG 
GACAGAGCAG AGGCAGCAGA GGCAGCTGAA GAGAGGCTTG ACTGTTGTAC TGATGGATGC 
TCAGATTCCT TGTTAGGACA TAATGAAGAG GCTGCTGGTC ACAATGGTGA GACCAGGACC 
TCCAGACCTG GTGGCACCCG GGCCAGCTGT GGAGGTAGAG TCACTGGAGG CAGCAGCAGC 
AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA CCCCCGAACC AGAGGTTCAG CAGCCGGTGG 
GAGCCCACCA TGGGTGAGCC CTCCAGCAGC ATCCCCACCA CTGTTGGCTC ACTTCCCAGT 
TCCAAAAGCT TCTTGGGCAT GAAGACCCGG GAGCTGTTCC GTAATAAGAG TGAGAGCCAG 
TGTGATGAGG ATGGCATGAC CATGAGTAGC TTTTCTGAGA CCCTGAAGAC AGAACTGGGC 
AAAGACTCGG CGGGCATGGA AAACAAGACT CCCCCAAGTC TAGACGCCCC ACACCCATCT 
TCCCCAAGCT CAGACAGTAT GGGGCAGCTC CATATCATGG ACTACAATGA GACTCATCAC 
GAACACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233908.1
CDS	2542..6033
Translation	MAQLANIGELLSMLDSSTLGVRDDVTTIFKESLNSERGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAATRLSILSLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVVVLTTGVLVLITMLPMIPQSGKQHLLDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENVETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKTLETHDVVIECAKISLDPTEASYEDGDAVSHQLSACFPHRSADVTTSSYVDTQNSYGGATSTPSSTSRLMLFSTPGQLPQSLSSLSTRPLPEPLQASLWSPSAVCGMTTPPTSPGNVPADLSHPYSKAFGTTTGGKGTPSGTPATSPPPAPPCPQDDCAHGPASQASATPPRKEERADSSRPYLPRQQDVPSDRGLEDLPGSKGSVTLRNLPDFLGDLASEEDSIEKDKEEAAISKELSEITTAEADPVAPRGGFDSPFYRDSLSGSQRKTHSAASGTQGFSVNPEPLHSSLDKHGPDTPKQAFTPIDPPSGSADASPAGDRDRQTSLETSILTPSPCKIPPQRGVSFGSGQLPPYDHLFEVALPKTACHFVSKKTEELLKKAKGNPEEDCVPSTSPMEVLDRLLEQGAGAHSKELSRLSLPSKSVDWTHFGGSPPSDEIRTLRDQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRAAALEEHNAAMKDQLKLQEKDIQMWKVSLQKEQARYSQLQQQRDTMVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRSMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQSKHPDTTKEVEMMKTAYRKELEKNRSHLLQQNQRLDASQRRVLELESLLAKKDHLLLEQKKYLEDVKSQASGQLLAAESRYEAQRKITRVLELEILDLYGRLEKDGRLQKLEEDRAEAAEAAEERLDCCTDGCSDSLLGHNEEAAGHNGETRTSRPGGTRASCGGRVTGGSSSSSSELSTPEKPPNQRFSSRWEPTMGEPSSSIPTTVGSLPSSKSFLGMKTRELFRNKSESQCDEDGMTMSSFSETLKTELGKDSAGMENKTPPSLDAPHPSSPSSDSMGQLHIMDYNETHHEHS

Target ORF information:

RefSeq Version	XM_006233846.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tuberous sclerosis 1 (Tsc1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233846.2

ORF Insert Sequence:

ATGGCCCAGC TAGCCAACAT TGGGGAGCTA CTCTCCATGC TGGACTCTTC CACACTGGGT 
GTGCGTGATG ACGTGACAAC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG 
CTTGTAAACA CGTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT CTTGCACATC 
CTGACCACCC TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAGTATGTA 
GGCAAAGCTG CCACCCGCTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTACT GAAATGTCTC 
AAGATGGACA CTGATGTTGT GGTTCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTA 
CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT 
CTTTCATCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTACCT GGTCCACCTC 
CATGCCAGTG TTTATGCCCT CTTCCATCGC CTTTATGGAA TGTACCCTTG TAACTTCGTC 
TCCTTCCTGC GCTCTCACTA CAGCATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCTAC CGAAGCCTCA TATGAAGATG GCGATGCTGT GTCTCACCAG 
CTCTCTGCCT GCTTCCCTCA CCGTTCAGCT GATGTCACCA CCAGCTCTTA CGTGGACACA 
CAGAATAGCT ATGGGGGCGC TACTTCCACC CCTTCCTCCA CCTCTCGGCT GATGTTGTTC 
AGTACCCCCG GGCAGCTACC TCAGAGTTTG AGTTCACTAT CAACACGGCC GTTACCTGAG 
CCGCTGCAAG CTAGTCTTTG GAGCCCATCT GCAGTCTGTG GTATGACCAC TCCTCCTACG 
TCTCCTGGAA ATGTCCCGGC TGATTTGTCA CATCCATACA GTAAAGCCTT TGGTACTACC 
ACTGGTGGAA AAGGAACTCC TTCAGGAACC CCAGCGACCT CTCCTCCCCC AGCCCCGCCT 
TGTCCCCAAG ACGACTGTGC GCACGGTCCA GCCTCCCAGG CCTCAGCCAC ACCCCCCAGG 
AAGGAAGAAA GAGCAGATTC TTCAAGGCCT TACCTACCCA GACAGCAGGA TGTTCCGAGT 
GACCGCGGAT TAGAGGATCT ACCTGGAAGC AAAGGTTCCG TCACTCTGAG GAATCTACCA 
GATTTCCTAG GTGACCTGGC TTCAGAGGAA GACAGTATAG AGAAAGATAA GGAAGAAGCT 
GCGATATCTA AAGAGCTTTC TGAGATCACC ACTGCAGAGG CGGATCCTGT AGCTCCTCGA 
GGGGGCTTTG ACTCTCCCTT CTACCGAGAC AGTCTCTCTG GTTCTCAGCG GAAGACTCAC 
TCAGCAGCCT CTGGGACTCA GGGCTTCAGC GTGAACCCTG AGCCTTTGCA CTCCTCCCTG 
GACAAACATG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCC ACCCTCTGGC 
AGTGCTGATG CCAGTCCCGC TGGGGACAGG GATCGCCAGA CTTCTCTGGA GACCAGTATC 
CTCACTCCCA GCCCTTGCAA AATTCCACCT CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG 
CTTCCCCCAT ATGATCATCT CTTTGAGGTG GCCTTGCCAA AGACTGCCTG TCACTTTGTC 
AGCAAAAAGA CTGAAGAGCT GTTGAAGAAA GCGAAAGGAA ACCCTGAGGA AGACTGTGTG 
CCCTCTACCT CCCCAATGGA AGTACTGGAC AGACTGCTAG AGCAGGGAGC AGGGGCACAC 
AGCAAGGAAC TGAGCAGGTT GTCCTTGCCC AGCAAGTCTG TTGACTGGAC CCACTTTGGA 
GGCTCTCCCC CCTCAGATGA GATCCGGACC CTCCGAGACC AGTTGCTCTT ACTGCACAAT 
CAGCTGCTGT ACGAACGCTT TAAGCGGCAG CAGCATGCAC TGAGGAACAG AAGGCTGCTG 
CGCAAGGTGA TCCGAGCTGC GGCCCTGGAG GAGCACAACG CAGCGATGAA AGATCAGTTG 
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA 
TACAGTCAGC TTCAGCAACA ACGTGACACC ATGGTGACCC AGCTGCATAG CCAAATCAGA 
CAGCTGCAGC ATGACCGAGA AGAATTCTAC AACCAGAGTC AGGAGTTACA GACGAAGCTG 
GAGGACTGCA GAAGCATGAT CGCGGAGCTT CGAGTGGAGC TGAAGAAGGC CAACAGCAAG 
GTGTGCCACA CCGAGCTGCT GCTCAGCCAG GTCTCTCAGA AGCTCTCCAA TAGTGAGTCA 
GTGCAGCAGC AGATGGAGTT CTTGAATAGG CAGCTTCTGG TGCTCGGGGA GGTCAATGAG 
CTGTACCTGG AGCAGCTGCA GAGCAAGCAT CCTGACACCA CCAAGGAAGT AGAAATGATG 
AAAACTGCTT ATCGGAAAGA ACTAGAGAAA AACAGAAGCC ACCTTCTCCA GCAGAACCAG 
AGGCTGGATG CCTCACAGAG GCGAGTTTTA GAACTGGAAT CTCTTCTGGC CAAGAAAGAC 
CACCTTCTCC TAGAGCAGAA GAAGTACCTT GAGGATGTCA AGAGCCAGGC GAGTGGACAG 
CTGCTGGCTG CAGAGAGCAG GTATGAGGCT CAGAGAAAGA TCACCCGGGT GTTGGAACTG 
GAGATCCTAG ACTTGTATGG TAGATTGGAG AAAGATGGCC GCCTACAGAA ACTAGAAGAG 
GACAGAGCAG AGGCAGCAGA GGCAGCTGAA GAGAGGCTTG ACTGTTGTAC TGATGGATGC 
TCAGATTCCT TGTTAGGACA TAATGAAGAG GCTGCTGGTC ACAATGGTGA GACCAGGACC 
TCCAGACCTG GTGGCACCCG GGCCAGCTGT GGAGGTAGAG TCACTGGAGG CAGCAGCAGC 
AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA CCCCCGAACC AGAGGTTCAG CAGCCGGTGG 
GAGCCCACCA TGGGTGAGCC CTCCAGCAGC ATCCCCACCA CTGTTGGCTC ACTTCCCAGT 
TCCAAAAGCT TCTTGGGCAT GAAGACCCGG GAGCTGTTCC GTAATAAGAG TGAGAGCCAG 
TGTGATGAGG ATGGCATGAC CATGAGTAGC TTTTCTGAGA CCCTGAAGAC AGAACTGGGC 
AAAGACTCGG CGGGCATGGA AAACAAGACT CCCCCAAGTC TAGACGCCCC ACACCCATCT 
TCCCCAAGCT CAGACAGTAT GGGGCAGCTC CATATCATGG ACTACAATGA GACTCATCAC 
GAACACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc1 ( XM_006233845.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233907.1 Rattus norvegicus tuberous sclerosis 1 (Tsc1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa25661
Clone ID Related Accession (Same CDS sequence)	XM_006233844.2 , XM_006233846.2 , XM_006233845.2 , XM_006233847.3 , XM_006233843.2
Accession Version	XM_006233845.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006233845.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC TAGCCAACAT TGGGGAGCTA CTCTCCATGC TGGACTCTTC CACACTGGGT 
GTGCGTGATG ACGTGACAAC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG 
CTTGTAAACA CGTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT CTTGCACATC 
CTGACCACCC TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAGTATGTA 
GGCAAAGCTG CCACCCGCTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTACT GAAATGTCTC 
AAGATGGACA CTGATGTTGT GGTTCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTA 
CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT 
CTTTCATCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTACCT GGTCCACCTC 
CATGCCAGTG TTTATGCCCT CTTCCATCGC CTTTATGGAA TGTACCCTTG TAACTTCGTC 
TCCTTCCTGC GCTCTCACTA CAGCATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCTAC CGAAGCCTCA TATGAAGATG GCGATGCTGT GTCTCACCAG 
CTCTCTGCCT GCTTCCCTCA CCGTTCAGCT GATGTCACCA CCAGCTCTTA CGTGGACACA 
CAGAATAGCT ATGGGGGCGC TACTTCCACC CCTTCCTCCA CCTCTCGGCT GATGTTGTTC 
AGTACCCCCG GGCAGCTACC TCAGAGTTTG AGTTCACTAT CAACACGGCC GTTACCTGAG 
CCGCTGCAAG CTAGTCTTTG GAGCCCATCT GCAGTCTGTG GTATGACCAC TCCTCCTACG 
TCTCCTGGAA ATGTCCCGGC TGATTTGTCA CATCCATACA GTAAAGCCTT TGGTACTACC 
ACTGGTGGAA AAGGAACTCC TTCAGGAACC CCAGCGACCT CTCCTCCCCC AGCCCCGCCT 
TGTCCCCAAG ACGACTGTGC GCACGGTCCA GCCTCCCAGG CCTCAGCCAC ACCCCCCAGG 
AAGGAAGAAA GAGCAGATTC TTCAAGGCCT TACCTACCCA GACAGCAGGA TGTTCCGAGT 
GACCGCGGAT TAGAGGATCT ACCTGGAAGC AAAGGTTCCG TCACTCTGAG GAATCTACCA 
GATTTCCTAG GTGACCTGGC TTCAGAGGAA GACAGTATAG AGAAAGATAA GGAAGAAGCT 
GCGATATCTA AAGAGCTTTC TGAGATCACC ACTGCAGAGG CGGATCCTGT AGCTCCTCGA 
GGGGGCTTTG ACTCTCCCTT CTACCGAGAC AGTCTCTCTG GTTCTCAGCG GAAGACTCAC 
TCAGCAGCCT CTGGGACTCA GGGCTTCAGC GTGAACCCTG AGCCTTTGCA CTCCTCCCTG 
GACAAACATG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCC ACCCTCTGGC 
AGTGCTGATG CCAGTCCCGC TGGGGACAGG GATCGCCAGA CTTCTCTGGA GACCAGTATC 
CTCACTCCCA GCCCTTGCAA AATTCCACCT CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG 
CTTCCCCCAT ATGATCATCT CTTTGAGGTG GCCTTGCCAA AGACTGCCTG TCACTTTGTC 
AGCAAAAAGA CTGAAGAGCT GTTGAAGAAA GCGAAAGGAA ACCCTGAGGA AGACTGTGTG 
CCCTCTACCT CCCCAATGGA AGTACTGGAC AGACTGCTAG AGCAGGGAGC AGGGGCACAC 
AGCAAGGAAC TGAGCAGGTT GTCCTTGCCC AGCAAGTCTG TTGACTGGAC CCACTTTGGA 
GGCTCTCCCC CCTCAGATGA GATCCGGACC CTCCGAGACC AGTTGCTCTT ACTGCACAAT 
CAGCTGCTGT ACGAACGCTT TAAGCGGCAG CAGCATGCAC TGAGGAACAG AAGGCTGCTG 
CGCAAGGTGA TCCGAGCTGC GGCCCTGGAG GAGCACAACG CAGCGATGAA AGATCAGTTG 
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA 
TACAGTCAGC TTCAGCAACA ACGTGACACC ATGGTGACCC AGCTGCATAG CCAAATCAGA 
CAGCTGCAGC ATGACCGAGA AGAATTCTAC AACCAGAGTC AGGAGTTACA GACGAAGCTG 
GAGGACTGCA GAAGCATGAT CGCGGAGCTT CGAGTGGAGC TGAAGAAGGC CAACAGCAAG 
GTGTGCCACA CCGAGCTGCT GCTCAGCCAG GTCTCTCAGA AGCTCTCCAA TAGTGAGTCA 
GTGCAGCAGC AGATGGAGTT CTTGAATAGG CAGCTTCTGG TGCTCGGGGA GGTCAATGAG 
CTGTACCTGG AGCAGCTGCA GAGCAAGCAT CCTGACACCA CCAAGGAAGT AGAAATGATG 
AAAACTGCTT ATCGGAAAGA ACTAGAGAAA AACAGAAGCC ACCTTCTCCA GCAGAACCAG 
AGGCTGGATG CCTCACAGAG GCGAGTTTTA GAACTGGAAT CTCTTCTGGC CAAGAAAGAC 
CACCTTCTCC TAGAGCAGAA GAAGTACCTT GAGGATGTCA AGAGCCAGGC GAGTGGACAG 
CTGCTGGCTG CAGAGAGCAG GTATGAGGCT CAGAGAAAGA TCACCCGGGT GTTGGAACTG 
GAGATCCTAG ACTTGTATGG TAGATTGGAG AAAGATGGCC GCCTACAGAA ACTAGAAGAG 
GACAGAGCAG AGGCAGCAGA GGCAGCTGAA GAGAGGCTTG ACTGTTGTAC TGATGGATGC 
TCAGATTCCT TGTTAGGACA TAATGAAGAG GCTGCTGGTC ACAATGGTGA GACCAGGACC 
TCCAGACCTG GTGGCACCCG GGCCAGCTGT GGAGGTAGAG TCACTGGAGG CAGCAGCAGC 
AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA CCCCCGAACC AGAGGTTCAG CAGCCGGTGG 
GAGCCCACCA TGGGTGAGCC CTCCAGCAGC ATCCCCACCA CTGTTGGCTC ACTTCCCAGT 
TCCAAAAGCT TCTTGGGCAT GAAGACCCGG GAGCTGTTCC GTAATAAGAG TGAGAGCCAG 
TGTGATGAGG ATGGCATGAC CATGAGTAGC TTTTCTGAGA CCCTGAAGAC AGAACTGGGC 
AAAGACTCGG CGGGCATGGA AAACAAGACT CCCCCAAGTC TAGACGCCCC ACACCCATCT 
TCCCCAAGCT CAGACAGTAT GGGGCAGCTC CATATCATGG ACTACAATGA GACTCATCAC 
GAACACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233907.1
CDS	277..3768
Translation	MAQLANIGELLSMLDSSTLGVRDDVTTIFKESLNSERGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAATRLSILSLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVVVLTTGVLVLITMLPMIPQSGKQHLLDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENVETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKTLETHDVVIECAKISLDPTEASYEDGDAVSHQLSACFPHRSADVTTSSYVDTQNSYGGATSTPSSTSRLMLFSTPGQLPQSLSSLSTRPLPEPLQASLWSPSAVCGMTTPPTSPGNVPADLSHPYSKAFGTTTGGKGTPSGTPATSPPPAPPCPQDDCAHGPASQASATPPRKEERADSSRPYLPRQQDVPSDRGLEDLPGSKGSVTLRNLPDFLGDLASEEDSIEKDKEEAAISKELSEITTAEADPVAPRGGFDSPFYRDSLSGSQRKTHSAASGTQGFSVNPEPLHSSLDKHGPDTPKQAFTPIDPPSGSADASPAGDRDRQTSLETSILTPSPCKIPPQRGVSFGSGQLPPYDHLFEVALPKTACHFVSKKTEELLKKAKGNPEEDCVPSTSPMEVLDRLLEQGAGAHSKELSRLSLPSKSVDWTHFGGSPPSDEIRTLRDQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRAAALEEHNAAMKDQLKLQEKDIQMWKVSLQKEQARYSQLQQQRDTMVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRSMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQSKHPDTTKEVEMMKTAYRKELEKNRSHLLQQNQRLDASQRRVLELESLLAKKDHLLLEQKKYLEDVKSQASGQLLAAESRYEAQRKITRVLELEILDLYGRLEKDGRLQKLEEDRAEAAEAAEERLDCCTDGCSDSLLGHNEEAAGHNGETRTSRPGGTRASCGGRVTGGSSSSSSELSTPEKPPNQRFSSRWEPTMGEPSSSIPTTVGSLPSSKSFLGMKTRELFRNKSESQCDEDGMTMSSFSETLKTELGKDSAGMENKTPPSLDAPHPSSPSSDSMGQLHIMDYNETHHEHS

Target ORF information:

RefSeq Version	XM_006233845.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tuberous sclerosis 1 (Tsc1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233845.2

ORF Insert Sequence:

ATGGCCCAGC TAGCCAACAT TGGGGAGCTA CTCTCCATGC TGGACTCTTC CACACTGGGT 
GTGCGTGATG ACGTGACAAC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG 
CTTGTAAACA CGTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT CTTGCACATC 
CTGACCACCC TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAGTATGTA 
GGCAAAGCTG CCACCCGCTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTACT GAAATGTCTC 
AAGATGGACA CTGATGTTGT GGTTCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTA 
CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT 
CTTTCATCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTACCT GGTCCACCTC 
CATGCCAGTG TTTATGCCCT CTTCCATCGC CTTTATGGAA TGTACCCTTG TAACTTCGTC 
TCCTTCCTGC GCTCTCACTA CAGCATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCTAC CGAAGCCTCA TATGAAGATG GCGATGCTGT GTCTCACCAG 
CTCTCTGCCT GCTTCCCTCA CCGTTCAGCT GATGTCACCA CCAGCTCTTA CGTGGACACA 
CAGAATAGCT ATGGGGGCGC TACTTCCACC CCTTCCTCCA CCTCTCGGCT GATGTTGTTC 
AGTACCCCCG GGCAGCTACC TCAGAGTTTG AGTTCACTAT CAACACGGCC GTTACCTGAG 
CCGCTGCAAG CTAGTCTTTG GAGCCCATCT GCAGTCTGTG GTATGACCAC TCCTCCTACG 
TCTCCTGGAA ATGTCCCGGC TGATTTGTCA CATCCATACA GTAAAGCCTT TGGTACTACC 
ACTGGTGGAA AAGGAACTCC TTCAGGAACC CCAGCGACCT CTCCTCCCCC AGCCCCGCCT 
TGTCCCCAAG ACGACTGTGC GCACGGTCCA GCCTCCCAGG CCTCAGCCAC ACCCCCCAGG 
AAGGAAGAAA GAGCAGATTC TTCAAGGCCT TACCTACCCA GACAGCAGGA TGTTCCGAGT 
GACCGCGGAT TAGAGGATCT ACCTGGAAGC AAAGGTTCCG TCACTCTGAG GAATCTACCA 
GATTTCCTAG GTGACCTGGC TTCAGAGGAA GACAGTATAG AGAAAGATAA GGAAGAAGCT 
GCGATATCTA AAGAGCTTTC TGAGATCACC ACTGCAGAGG CGGATCCTGT AGCTCCTCGA 
GGGGGCTTTG ACTCTCCCTT CTACCGAGAC AGTCTCTCTG GTTCTCAGCG GAAGACTCAC 
TCAGCAGCCT CTGGGACTCA GGGCTTCAGC GTGAACCCTG AGCCTTTGCA CTCCTCCCTG 
GACAAACATG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCC ACCCTCTGGC 
AGTGCTGATG CCAGTCCCGC TGGGGACAGG GATCGCCAGA CTTCTCTGGA GACCAGTATC 
CTCACTCCCA GCCCTTGCAA AATTCCACCT CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG 
CTTCCCCCAT ATGATCATCT CTTTGAGGTG GCCTTGCCAA AGACTGCCTG TCACTTTGTC 
AGCAAAAAGA CTGAAGAGCT GTTGAAGAAA GCGAAAGGAA ACCCTGAGGA AGACTGTGTG 
CCCTCTACCT CCCCAATGGA AGTACTGGAC AGACTGCTAG AGCAGGGAGC AGGGGCACAC 
AGCAAGGAAC TGAGCAGGTT GTCCTTGCCC AGCAAGTCTG TTGACTGGAC CCACTTTGGA 
GGCTCTCCCC CCTCAGATGA GATCCGGACC CTCCGAGACC AGTTGCTCTT ACTGCACAAT 
CAGCTGCTGT ACGAACGCTT TAAGCGGCAG CAGCATGCAC TGAGGAACAG AAGGCTGCTG 
CGCAAGGTGA TCCGAGCTGC GGCCCTGGAG GAGCACAACG CAGCGATGAA AGATCAGTTG 
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA 
TACAGTCAGC TTCAGCAACA ACGTGACACC ATGGTGACCC AGCTGCATAG CCAAATCAGA 
CAGCTGCAGC ATGACCGAGA AGAATTCTAC AACCAGAGTC AGGAGTTACA GACGAAGCTG 
GAGGACTGCA GAAGCATGAT CGCGGAGCTT CGAGTGGAGC TGAAGAAGGC CAACAGCAAG 
GTGTGCCACA CCGAGCTGCT GCTCAGCCAG GTCTCTCAGA AGCTCTCCAA TAGTGAGTCA 
GTGCAGCAGC AGATGGAGTT CTTGAATAGG CAGCTTCTGG TGCTCGGGGA GGTCAATGAG 
CTGTACCTGG AGCAGCTGCA GAGCAAGCAT CCTGACACCA CCAAGGAAGT AGAAATGATG 
AAAACTGCTT ATCGGAAAGA ACTAGAGAAA AACAGAAGCC ACCTTCTCCA GCAGAACCAG 
AGGCTGGATG CCTCACAGAG GCGAGTTTTA GAACTGGAAT CTCTTCTGGC CAAGAAAGAC 
CACCTTCTCC TAGAGCAGAA GAAGTACCTT GAGGATGTCA AGAGCCAGGC GAGTGGACAG 
CTGCTGGCTG CAGAGAGCAG GTATGAGGCT CAGAGAAAGA TCACCCGGGT GTTGGAACTG 
GAGATCCTAG ACTTGTATGG TAGATTGGAG AAAGATGGCC GCCTACAGAA ACTAGAAGAG 
GACAGAGCAG AGGCAGCAGA GGCAGCTGAA GAGAGGCTTG ACTGTTGTAC TGATGGATGC 
TCAGATTCCT TGTTAGGACA TAATGAAGAG GCTGCTGGTC ACAATGGTGA GACCAGGACC 
TCCAGACCTG GTGGCACCCG GGCCAGCTGT GGAGGTAGAG TCACTGGAGG CAGCAGCAGC 
AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA CCCCCGAACC AGAGGTTCAG CAGCCGGTGG 
GAGCCCACCA TGGGTGAGCC CTCCAGCAGC ATCCCCACCA CTGTTGGCTC ACTTCCCAGT 
TCCAAAAGCT TCTTGGGCAT GAAGACCCGG GAGCTGTTCC GTAATAAGAG TGAGAGCCAG 
TGTGATGAGG ATGGCATGAC CATGAGTAGC TTTTCTGAGA CCCTGAAGAC AGAACTGGGC 
AAAGACTCGG CGGGCATGGA AAACAAGACT CCCCCAAGTC TAGACGCCCC ACACCCATCT 
TCCCCAAGCT CAGACAGTAT GGGGCAGCTC CATATCATGG ACTACAATGA GACTCATCAC 
GAACACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc1 ( XM_006233847.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233909.1 Rattus norvegicus tuberous sclerosis 1 (Tsc1), transcript variant X4, mRNA.

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CloneID	ORa25661
Clone ID Related Accession (Same CDS sequence)	XM_006233844.2 , XM_006233846.2 , XM_006233845.2 , XM_006233847.3 , XM_006233843.2
Accession Version	XM_006233847.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233847.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC TAGCCAACAT TGGGGAGCTA CTCTCCATGC TGGACTCTTC CACACTGGGT 
GTGCGTGATG ACGTGACAAC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG 
CTTGTAAACA CGTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT CTTGCACATC 
CTGACCACCC TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAGTATGTA 
GGCAAAGCTG CCACCCGCTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTACT GAAATGTCTC 
AAGATGGACA CTGATGTTGT GGTTCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTA 
CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT 
CTTTCATCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTACCT GGTCCACCTC 
CATGCCAGTG TTTATGCCCT CTTCCATCGC CTTTATGGAA TGTACCCTTG TAACTTCGTC 
TCCTTCCTGC GCTCTCACTA CAGCATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCTAC CGAAGCCTCA TATGAAGATG GCGATGCTGT GTCTCACCAG 
CTCTCTGCCT GCTTCCCTCA CCGTTCAGCT GATGTCACCA CCAGCTCTTA CGTGGACACA 
CAGAATAGCT ATGGGGGCGC TACTTCCACC CCTTCCTCCA CCTCTCGGCT GATGTTGTTC 
AGTACCCCCG GGCAGCTACC TCAGAGTTTG AGTTCACTAT CAACACGGCC GTTACCTGAG 
CCGCTGCAAG CTAGTCTTTG GAGCCCATCT GCAGTCTGTG GTATGACCAC TCCTCCTACG 
TCTCCTGGAA ATGTCCCGGC TGATTTGTCA CATCCATACA GTAAAGCCTT TGGTACTACC 
ACTGGTGGAA AAGGAACTCC TTCAGGAACC CCAGCGACCT CTCCTCCCCC AGCCCCGCCT 
TGTCCCCAAG ACGACTGTGC GCACGGTCCA GCCTCCCAGG CCTCAGCCAC ACCCCCCAGG 
AAGGAAGAAA GAGCAGATTC TTCAAGGCCT TACCTACCCA GACAGCAGGA TGTTCCGAGT 
GACCGCGGAT TAGAGGATCT ACCTGGAAGC AAAGGTTCCG TCACTCTGAG GAATCTACCA 
GATTTCCTAG GTGACCTGGC TTCAGAGGAA GACAGTATAG AGAAAGATAA GGAAGAAGCT 
GCGATATCTA AAGAGCTTTC TGAGATCACC ACTGCAGAGG CGGATCCTGT AGCTCCTCGA 
GGGGGCTTTG ACTCTCCCTT CTACCGAGAC AGTCTCTCTG GTTCTCAGCG GAAGACTCAC 
TCAGCAGCCT CTGGGACTCA GGGCTTCAGC GTGAACCCTG AGCCTTTGCA CTCCTCCCTG 
GACAAACATG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCC ACCCTCTGGC 
AGTGCTGATG CCAGTCCCGC TGGGGACAGG GATCGCCAGA CTTCTCTGGA GACCAGTATC 
CTCACTCCCA GCCCTTGCAA AATTCCACCT CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG 
CTTCCCCCAT ATGATCATCT CTTTGAGGTG GCCTTGCCAA AGACTGCCTG TCACTTTGTC 
AGCAAAAAGA CTGAAGAGCT GTTGAAGAAA GCGAAAGGAA ACCCTGAGGA AGACTGTGTG 
CCCTCTACCT CCCCAATGGA AGTACTGGAC AGACTGCTAG AGCAGGGAGC AGGGGCACAC 
AGCAAGGAAC TGAGCAGGTT GTCCTTGCCC AGCAAGTCTG TTGACTGGAC CCACTTTGGA 
GGCTCTCCCC CCTCAGATGA GATCCGGACC CTCCGAGACC AGTTGCTCTT ACTGCACAAT 
CAGCTGCTGT ACGAACGCTT TAAGCGGCAG CAGCATGCAC TGAGGAACAG AAGGCTGCTG 
CGCAAGGTGA TCCGAGCTGC GGCCCTGGAG GAGCACAACG CAGCGATGAA AGATCAGTTG 
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA 
TACAGTCAGC TTCAGCAACA ACGTGACACC ATGGTGACCC AGCTGCATAG CCAAATCAGA 
CAGCTGCAGC ATGACCGAGA AGAATTCTAC AACCAGAGTC AGGAGTTACA GACGAAGCTG 
GAGGACTGCA GAAGCATGAT CGCGGAGCTT CGAGTGGAGC TGAAGAAGGC CAACAGCAAG 
GTGTGCCACA CCGAGCTGCT GCTCAGCCAG GTCTCTCAGA AGCTCTCCAA TAGTGAGTCA 
GTGCAGCAGC AGATGGAGTT CTTGAATAGG CAGCTTCTGG TGCTCGGGGA GGTCAATGAG 
CTGTACCTGG AGCAGCTGCA GAGCAAGCAT CCTGACACCA CCAAGGAAGT AGAAATGATG 
AAAACTGCTT ATCGGAAAGA ACTAGAGAAA AACAGAAGCC ACCTTCTCCA GCAGAACCAG 
AGGCTGGATG CCTCACAGAG GCGAGTTTTA GAACTGGAAT CTCTTCTGGC CAAGAAAGAC 
CACCTTCTCC TAGAGCAGAA GAAGTACCTT GAGGATGTCA AGAGCCAGGC GAGTGGACAG 
CTGCTGGCTG CAGAGAGCAG GTATGAGGCT CAGAGAAAGA TCACCCGGGT GTTGGAACTG 
GAGATCCTAG ACTTGTATGG TAGATTGGAG AAAGATGGCC GCCTACAGAA ACTAGAAGAG 
GACAGAGCAG AGGCAGCAGA GGCAGCTGAA GAGAGGCTTG ACTGTTGTAC TGATGGATGC 
TCAGATTCCT TGTTAGGACA TAATGAAGAG GCTGCTGGTC ACAATGGTGA GACCAGGACC 
TCCAGACCTG GTGGCACCCG GGCCAGCTGT GGAGGTAGAG TCACTGGAGG CAGCAGCAGC 
AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA CCCCCGAACC AGAGGTTCAG CAGCCGGTGG 
GAGCCCACCA TGGGTGAGCC CTCCAGCAGC ATCCCCACCA CTGTTGGCTC ACTTCCCAGT 
TCCAAAAGCT TCTTGGGCAT GAAGACCCGG GAGCTGTTCC GTAATAAGAG TGAGAGCCAG 
TGTGATGAGG ATGGCATGAC CATGAGTAGC TTTTCTGAGA CCCTGAAGAC AGAACTGGGC 
AAAGACTCGG CGGGCATGGA AAACAAGACT CCCCCAAGTC TAGACGCCCC ACACCCATCT 
TCCCCAAGCT CAGACAGTAT GGGGCAGCTC CATATCATGG ACTACAATGA GACTCATCAC 
GAACACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233909.1
CDS	209..3700
Translation	MAQLANIGELLSMLDSSTLGVRDDVTTIFKESLNSERGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAATRLSILSLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVVVLTTGVLVLITMLPMIPQSGKQHLLDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENVETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKTLETHDVVIECAKISLDPTEASYEDGDAVSHQLSACFPHRSADVTTSSYVDTQNSYGGATSTPSSTSRLMLFSTPGQLPQSLSSLSTRPLPEPLQASLWSPSAVCGMTTPPTSPGNVPADLSHPYSKAFGTTTGGKGTPSGTPATSPPPAPPCPQDDCAHGPASQASATPPRKEERADSSRPYLPRQQDVPSDRGLEDLPGSKGSVTLRNLPDFLGDLASEEDSIEKDKEEAAISKELSEITTAEADPVAPRGGFDSPFYRDSLSGSQRKTHSAASGTQGFSVNPEPLHSSLDKHGPDTPKQAFTPIDPPSGSADASPAGDRDRQTSLETSILTPSPCKIPPQRGVSFGSGQLPPYDHLFEVALPKTACHFVSKKTEELLKKAKGNPEEDCVPSTSPMEVLDRLLEQGAGAHSKELSRLSLPSKSVDWTHFGGSPPSDEIRTLRDQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRAAALEEHNAAMKDQLKLQEKDIQMWKVSLQKEQARYSQLQQQRDTMVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRSMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQSKHPDTTKEVEMMKTAYRKELEKNRSHLLQQNQRLDASQRRVLELESLLAKKDHLLLEQKKYLEDVKSQASGQLLAAESRYEAQRKITRVLELEILDLYGRLEKDGRLQKLEEDRAEAAEAAEERLDCCTDGCSDSLLGHNEEAAGHNGETRTSRPGGTRASCGGRVTGGSSSSSSELSTPEKPPNQRFSSRWEPTMGEPSSSIPTTVGSLPSSKSFLGMKTRELFRNKSESQCDEDGMTMSSFSETLKTELGKDSAGMENKTPPSLDAPHPSSPSSDSMGQLHIMDYNETHHEHS

Target ORF information:

RefSeq Version	XM_006233847.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tuberous sclerosis 1 (Tsc1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233847.3

ORF Insert Sequence:

ATGGCCCAGC TAGCCAACAT TGGGGAGCTA CTCTCCATGC TGGACTCTTC CACACTGGGT 
GTGCGTGATG ACGTGACAAC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG 
CTTGTAAACA CGTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT CTTGCACATC 
CTGACCACCC TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAGTATGTA 
GGCAAAGCTG CCACCCGCTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTACT GAAATGTCTC 
AAGATGGACA CTGATGTTGT GGTTCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTA 
CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT 
CTTTCATCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTACCT GGTCCACCTC 
CATGCCAGTG TTTATGCCCT CTTCCATCGC CTTTATGGAA TGTACCCTTG TAACTTCGTC 
TCCTTCCTGC GCTCTCACTA CAGCATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCTAC CGAAGCCTCA TATGAAGATG GCGATGCTGT GTCTCACCAG 
CTCTCTGCCT GCTTCCCTCA CCGTTCAGCT GATGTCACCA CCAGCTCTTA CGTGGACACA 
CAGAATAGCT ATGGGGGCGC TACTTCCACC CCTTCCTCCA CCTCTCGGCT GATGTTGTTC 
AGTACCCCCG GGCAGCTACC TCAGAGTTTG AGTTCACTAT CAACACGGCC GTTACCTGAG 
CCGCTGCAAG CTAGTCTTTG GAGCCCATCT GCAGTCTGTG GTATGACCAC TCCTCCTACG 
TCTCCTGGAA ATGTCCCGGC TGATTTGTCA CATCCATACA GTAAAGCCTT TGGTACTACC 
ACTGGTGGAA AAGGAACTCC TTCAGGAACC CCAGCGACCT CTCCTCCCCC AGCCCCGCCT 
TGTCCCCAAG ACGACTGTGC GCACGGTCCA GCCTCCCAGG CCTCAGCCAC ACCCCCCAGG 
AAGGAAGAAA GAGCAGATTC TTCAAGGCCT TACCTACCCA GACAGCAGGA TGTTCCGAGT 
GACCGCGGAT TAGAGGATCT ACCTGGAAGC AAAGGTTCCG TCACTCTGAG GAATCTACCA 
GATTTCCTAG GTGACCTGGC TTCAGAGGAA GACAGTATAG AGAAAGATAA GGAAGAAGCT 
GCGATATCTA AAGAGCTTTC TGAGATCACC ACTGCAGAGG CGGATCCTGT AGCTCCTCGA 
GGGGGCTTTG ACTCTCCCTT CTACCGAGAC AGTCTCTCTG GTTCTCAGCG GAAGACTCAC 
TCAGCAGCCT CTGGGACTCA GGGCTTCAGC GTGAACCCTG AGCCTTTGCA CTCCTCCCTG 
GACAAACATG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCC ACCCTCTGGC 
AGTGCTGATG CCAGTCCCGC TGGGGACAGG GATCGCCAGA CTTCTCTGGA GACCAGTATC 
CTCACTCCCA GCCCTTGCAA AATTCCACCT CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG 
CTTCCCCCAT ATGATCATCT CTTTGAGGTG GCCTTGCCAA AGACTGCCTG TCACTTTGTC 
AGCAAAAAGA CTGAAGAGCT GTTGAAGAAA GCGAAAGGAA ACCCTGAGGA AGACTGTGTG 
CCCTCTACCT CCCCAATGGA AGTACTGGAC AGACTGCTAG AGCAGGGAGC AGGGGCACAC 
AGCAAGGAAC TGAGCAGGTT GTCCTTGCCC AGCAAGTCTG TTGACTGGAC CCACTTTGGA 
GGCTCTCCCC CCTCAGATGA GATCCGGACC CTCCGAGACC AGTTGCTCTT ACTGCACAAT 
CAGCTGCTGT ACGAACGCTT TAAGCGGCAG CAGCATGCAC TGAGGAACAG AAGGCTGCTG 
CGCAAGGTGA TCCGAGCTGC GGCCCTGGAG GAGCACAACG CAGCGATGAA AGATCAGTTG 
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA 
TACAGTCAGC TTCAGCAACA ACGTGACACC ATGGTGACCC AGCTGCATAG CCAAATCAGA 
CAGCTGCAGC ATGACCGAGA AGAATTCTAC AACCAGAGTC AGGAGTTACA GACGAAGCTG 
GAGGACTGCA GAAGCATGAT CGCGGAGCTT CGAGTGGAGC TGAAGAAGGC CAACAGCAAG 
GTGTGCCACA CCGAGCTGCT GCTCAGCCAG GTCTCTCAGA AGCTCTCCAA TAGTGAGTCA 
GTGCAGCAGC AGATGGAGTT CTTGAATAGG CAGCTTCTGG TGCTCGGGGA GGTCAATGAG 
CTGTACCTGG AGCAGCTGCA GAGCAAGCAT CCTGACACCA CCAAGGAAGT AGAAATGATG 
AAAACTGCTT ATCGGAAAGA ACTAGAGAAA AACAGAAGCC ACCTTCTCCA GCAGAACCAG 
AGGCTGGATG CCTCACAGAG GCGAGTTTTA GAACTGGAAT CTCTTCTGGC CAAGAAAGAC 
CACCTTCTCC TAGAGCAGAA GAAGTACCTT GAGGATGTCA AGAGCCAGGC GAGTGGACAG 
CTGCTGGCTG CAGAGAGCAG GTATGAGGCT CAGAGAAAGA TCACCCGGGT GTTGGAACTG 
GAGATCCTAG ACTTGTATGG TAGATTGGAG AAAGATGGCC GCCTACAGAA ACTAGAAGAG 
GACAGAGCAG AGGCAGCAGA GGCAGCTGAA GAGAGGCTTG ACTGTTGTAC TGATGGATGC 
TCAGATTCCT TGTTAGGACA TAATGAAGAG GCTGCTGGTC ACAATGGTGA GACCAGGACC 
TCCAGACCTG GTGGCACCCG GGCCAGCTGT GGAGGTAGAG TCACTGGAGG CAGCAGCAGC 
AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA CCCCCGAACC AGAGGTTCAG CAGCCGGTGG 
GAGCCCACCA TGGGTGAGCC CTCCAGCAGC ATCCCCACCA CTGTTGGCTC ACTTCCCAGT 
TCCAAAAGCT TCTTGGGCAT GAAGACCCGG GAGCTGTTCC GTAATAAGAG TGAGAGCCAG 
TGTGATGAGG ATGGCATGAC CATGAGTAGC TTTTCTGAGA CCCTGAAGAC AGAACTGGGC 
AAAGACTCGG CGGGCATGGA AAACAAGACT CCCCCAAGTC TAGACGCCCC ACACCCATCT 
TCCCCAAGCT CAGACAGTAT GGGGCAGCTC CATATCATGG ACTACAATGA GACTCATCAC 
GAACACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc1 ( XM_006233843.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233905.1 Rattus norvegicus tuberous sclerosis 1 (Tsc1), transcript variant X1, mRNA.

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CloneID	ORa25661
Clone ID Related Accession (Same CDS sequence)	XM_006233844.2 , XM_006233846.2 , XM_006233845.2 , XM_006233847.3 , XM_006233843.2
Accession Version	XM_006233843.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006233843.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC TAGCCAACAT TGGGGAGCTA CTCTCCATGC TGGACTCTTC CACACTGGGT 
GTGCGTGATG ACGTGACAAC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG 
CTTGTAAACA CGTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT CTTGCACATC 
CTGACCACCC TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAGTATGTA 
GGCAAAGCTG CCACCCGCTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTACT GAAATGTCTC 
AAGATGGACA CTGATGTTGT GGTTCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTA 
CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT 
CTTTCATCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTACCT GGTCCACCTC 
CATGCCAGTG TTTATGCCCT CTTCCATCGC CTTTATGGAA TGTACCCTTG TAACTTCGTC 
TCCTTCCTGC GCTCTCACTA CAGCATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCTAC CGAAGCCTCA TATGAAGATG GCGATGCTGT GTCTCACCAG 
CTCTCTGCCT GCTTCCCTCA CCGTTCAGCT GATGTCACCA CCAGCTCTTA CGTGGACACA 
CAGAATAGCT ATGGGGGCGC TACTTCCACC CCTTCCTCCA CCTCTCGGCT GATGTTGTTC 
AGTACCCCCG GGCAGCTACC TCAGAGTTTG AGTTCACTAT CAACACGGCC GTTACCTGAG 
CCGCTGCAAG CTAGTCTTTG GAGCCCATCT GCAGTCTGTG GTATGACCAC TCCTCCTACG 
TCTCCTGGAA ATGTCCCGGC TGATTTGTCA CATCCATACA GTAAAGCCTT TGGTACTACC 
ACTGGTGGAA AAGGAACTCC TTCAGGAACC CCAGCGACCT CTCCTCCCCC AGCCCCGCCT 
TGTCCCCAAG ACGACTGTGC GCACGGTCCA GCCTCCCAGG CCTCAGCCAC ACCCCCCAGG 
AAGGAAGAAA GAGCAGATTC TTCAAGGCCT TACCTACCCA GACAGCAGGA TGTTCCGAGT 
GACCGCGGAT TAGAGGATCT ACCTGGAAGC AAAGGTTCCG TCACTCTGAG GAATCTACCA 
GATTTCCTAG GTGACCTGGC TTCAGAGGAA GACAGTATAG AGAAAGATAA GGAAGAAGCT 
GCGATATCTA AAGAGCTTTC TGAGATCACC ACTGCAGAGG CGGATCCTGT AGCTCCTCGA 
GGGGGCTTTG ACTCTCCCTT CTACCGAGAC AGTCTCTCTG GTTCTCAGCG GAAGACTCAC 
TCAGCAGCCT CTGGGACTCA GGGCTTCAGC GTGAACCCTG AGCCTTTGCA CTCCTCCCTG 
GACAAACATG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCC ACCCTCTGGC 
AGTGCTGATG CCAGTCCCGC TGGGGACAGG GATCGCCAGA CTTCTCTGGA GACCAGTATC 
CTCACTCCCA GCCCTTGCAA AATTCCACCT CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG 
CTTCCCCCAT ATGATCATCT CTTTGAGGTG GCCTTGCCAA AGACTGCCTG TCACTTTGTC 
AGCAAAAAGA CTGAAGAGCT GTTGAAGAAA GCGAAAGGAA ACCCTGAGGA AGACTGTGTG 
CCCTCTACCT CCCCAATGGA AGTACTGGAC AGACTGCTAG AGCAGGGAGC AGGGGCACAC 
AGCAAGGAAC TGAGCAGGTT GTCCTTGCCC AGCAAGTCTG TTGACTGGAC CCACTTTGGA 
GGCTCTCCCC CCTCAGATGA GATCCGGACC CTCCGAGACC AGTTGCTCTT ACTGCACAAT 
CAGCTGCTGT ACGAACGCTT TAAGCGGCAG CAGCATGCAC TGAGGAACAG AAGGCTGCTG 
CGCAAGGTGA TCCGAGCTGC GGCCCTGGAG GAGCACAACG CAGCGATGAA AGATCAGTTG 
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA 
TACAGTCAGC TTCAGCAACA ACGTGACACC ATGGTGACCC AGCTGCATAG CCAAATCAGA 
CAGCTGCAGC ATGACCGAGA AGAATTCTAC AACCAGAGTC AGGAGTTACA GACGAAGCTG 
GAGGACTGCA GAAGCATGAT CGCGGAGCTT CGAGTGGAGC TGAAGAAGGC CAACAGCAAG 
GTGTGCCACA CCGAGCTGCT GCTCAGCCAG GTCTCTCAGA AGCTCTCCAA TAGTGAGTCA 
GTGCAGCAGC AGATGGAGTT CTTGAATAGG CAGCTTCTGG TGCTCGGGGA GGTCAATGAG 
CTGTACCTGG AGCAGCTGCA GAGCAAGCAT CCTGACACCA CCAAGGAAGT AGAAATGATG 
AAAACTGCTT ATCGGAAAGA ACTAGAGAAA AACAGAAGCC ACCTTCTCCA GCAGAACCAG 
AGGCTGGATG CCTCACAGAG GCGAGTTTTA GAACTGGAAT CTCTTCTGGC CAAGAAAGAC 
CACCTTCTCC TAGAGCAGAA GAAGTACCTT GAGGATGTCA AGAGCCAGGC GAGTGGACAG 
CTGCTGGCTG CAGAGAGCAG GTATGAGGCT CAGAGAAAGA TCACCCGGGT GTTGGAACTG 
GAGATCCTAG ACTTGTATGG TAGATTGGAG AAAGATGGCC GCCTACAGAA ACTAGAAGAG 
GACAGAGCAG AGGCAGCAGA GGCAGCTGAA GAGAGGCTTG ACTGTTGTAC TGATGGATGC 
TCAGATTCCT TGTTAGGACA TAATGAAGAG GCTGCTGGTC ACAATGGTGA GACCAGGACC 
TCCAGACCTG GTGGCACCCG GGCCAGCTGT GGAGGTAGAG TCACTGGAGG CAGCAGCAGC 
AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA CCCCCGAACC AGAGGTTCAG CAGCCGGTGG 
GAGCCCACCA TGGGTGAGCC CTCCAGCAGC ATCCCCACCA CTGTTGGCTC ACTTCCCAGT 
TCCAAAAGCT TCTTGGGCAT GAAGACCCGG GAGCTGTTCC GTAATAAGAG TGAGAGCCAG 
TGTGATGAGG ATGGCATGAC CATGAGTAGC TTTTCTGAGA CCCTGAAGAC AGAACTGGGC 
AAAGACTCGG CGGGCATGGA AAACAAGACT CCCCCAAGTC TAGACGCCCC ACACCCATCT 
TCCCCAAGCT CAGACAGTAT GGGGCAGCTC CATATCATGG ACTACAATGA GACTCATCAC 
GAACACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233905.1
CDS	455..3946
Translation	MAQLANIGELLSMLDSSTLGVRDDVTTIFKESLNSERGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAATRLSILSLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVVVLTTGVLVLITMLPMIPQSGKQHLLDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENVETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKTLETHDVVIECAKISLDPTEASYEDGDAVSHQLSACFPHRSADVTTSSYVDTQNSYGGATSTPSSTSRLMLFSTPGQLPQSLSSLSTRPLPEPLQASLWSPSAVCGMTTPPTSPGNVPADLSHPYSKAFGTTTGGKGTPSGTPATSPPPAPPCPQDDCAHGPASQASATPPRKEERADSSRPYLPRQQDVPSDRGLEDLPGSKGSVTLRNLPDFLGDLASEEDSIEKDKEEAAISKELSEITTAEADPVAPRGGFDSPFYRDSLSGSQRKTHSAASGTQGFSVNPEPLHSSLDKHGPDTPKQAFTPIDPPSGSADASPAGDRDRQTSLETSILTPSPCKIPPQRGVSFGSGQLPPYDHLFEVALPKTACHFVSKKTEELLKKAKGNPEEDCVPSTSPMEVLDRLLEQGAGAHSKELSRLSLPSKSVDWTHFGGSPPSDEIRTLRDQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRAAALEEHNAAMKDQLKLQEKDIQMWKVSLQKEQARYSQLQQQRDTMVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRSMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQSKHPDTTKEVEMMKTAYRKELEKNRSHLLQQNQRLDASQRRVLELESLLAKKDHLLLEQKKYLEDVKSQASGQLLAAESRYEAQRKITRVLELEILDLYGRLEKDGRLQKLEEDRAEAAEAAEERLDCCTDGCSDSLLGHNEEAAGHNGETRTSRPGGTRASCGGRVTGGSSSSSSELSTPEKPPNQRFSSRWEPTMGEPSSSIPTTVGSLPSSKSFLGMKTRELFRNKSESQCDEDGMTMSSFSETLKTELGKDSAGMENKTPPSLDAPHPSSPSSDSMGQLHIMDYNETHHEHS

Target ORF information:

RefSeq Version	XM_006233843.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tuberous sclerosis 1 (Tsc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233843.2

ORF Insert Sequence:

ATGGCCCAGC TAGCCAACAT TGGGGAGCTA CTCTCCATGC TGGACTCTTC CACACTGGGT 
GTGCGTGATG ACGTGACAAC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG 
CTTGTAAACA CGTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT CTTGCACATC 
CTGACCACCC TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAGTATGTA 
GGCAAAGCTG CCACCCGCTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTACT GAAATGTCTC 
AAGATGGACA CTGATGTTGT GGTTCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTA 
CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT 
CTTTCATCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTACCT GGTCCACCTC 
CATGCCAGTG TTTATGCCCT CTTCCATCGC CTTTATGGAA TGTACCCTTG TAACTTCGTC 
TCCTTCCTGC GCTCTCACTA CAGCATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCTAC CGAAGCCTCA TATGAAGATG GCGATGCTGT GTCTCACCAG 
CTCTCTGCCT GCTTCCCTCA CCGTTCAGCT GATGTCACCA CCAGCTCTTA CGTGGACACA 
CAGAATAGCT ATGGGGGCGC TACTTCCACC CCTTCCTCCA CCTCTCGGCT GATGTTGTTC 
AGTACCCCCG GGCAGCTACC TCAGAGTTTG AGTTCACTAT CAACACGGCC GTTACCTGAG 
CCGCTGCAAG CTAGTCTTTG GAGCCCATCT GCAGTCTGTG GTATGACCAC TCCTCCTACG 
TCTCCTGGAA ATGTCCCGGC TGATTTGTCA CATCCATACA GTAAAGCCTT TGGTACTACC 
ACTGGTGGAA AAGGAACTCC TTCAGGAACC CCAGCGACCT CTCCTCCCCC AGCCCCGCCT 
TGTCCCCAAG ACGACTGTGC GCACGGTCCA GCCTCCCAGG CCTCAGCCAC ACCCCCCAGG 
AAGGAAGAAA GAGCAGATTC TTCAAGGCCT TACCTACCCA GACAGCAGGA TGTTCCGAGT 
GACCGCGGAT TAGAGGATCT ACCTGGAAGC AAAGGTTCCG TCACTCTGAG GAATCTACCA 
GATTTCCTAG GTGACCTGGC TTCAGAGGAA GACAGTATAG AGAAAGATAA GGAAGAAGCT 
GCGATATCTA AAGAGCTTTC TGAGATCACC ACTGCAGAGG CGGATCCTGT AGCTCCTCGA 
GGGGGCTTTG ACTCTCCCTT CTACCGAGAC AGTCTCTCTG GTTCTCAGCG GAAGACTCAC 
TCAGCAGCCT CTGGGACTCA GGGCTTCAGC GTGAACCCTG AGCCTTTGCA CTCCTCCCTG 
GACAAACATG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCC ACCCTCTGGC 
AGTGCTGATG CCAGTCCCGC TGGGGACAGG GATCGCCAGA CTTCTCTGGA GACCAGTATC 
CTCACTCCCA GCCCTTGCAA AATTCCACCT CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG 
CTTCCCCCAT ATGATCATCT CTTTGAGGTG GCCTTGCCAA AGACTGCCTG TCACTTTGTC 
AGCAAAAAGA CTGAAGAGCT GTTGAAGAAA GCGAAAGGAA ACCCTGAGGA AGACTGTGTG 
CCCTCTACCT CCCCAATGGA AGTACTGGAC AGACTGCTAG AGCAGGGAGC AGGGGCACAC 
AGCAAGGAAC TGAGCAGGTT GTCCTTGCCC AGCAAGTCTG TTGACTGGAC CCACTTTGGA 
GGCTCTCCCC CCTCAGATGA GATCCGGACC CTCCGAGACC AGTTGCTCTT ACTGCACAAT 
CAGCTGCTGT ACGAACGCTT TAAGCGGCAG CAGCATGCAC TGAGGAACAG AAGGCTGCTG 
CGCAAGGTGA TCCGAGCTGC GGCCCTGGAG GAGCACAACG CAGCGATGAA AGATCAGTTG 
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTGAGTC TGCAGAAAGA ACAAGCCCGA 
TACAGTCAGC TTCAGCAACA ACGTGACACC ATGGTGACCC AGCTGCATAG CCAAATCAGA 
CAGCTGCAGC ATGACCGAGA AGAATTCTAC AACCAGAGTC AGGAGTTACA GACGAAGCTG 
GAGGACTGCA GAAGCATGAT CGCGGAGCTT CGAGTGGAGC TGAAGAAGGC CAACAGCAAG 
GTGTGCCACA CCGAGCTGCT GCTCAGCCAG GTCTCTCAGA AGCTCTCCAA TAGTGAGTCA 
GTGCAGCAGC AGATGGAGTT CTTGAATAGG CAGCTTCTGG TGCTCGGGGA GGTCAATGAG 
CTGTACCTGG AGCAGCTGCA GAGCAAGCAT CCTGACACCA CCAAGGAAGT AGAAATGATG 
AAAACTGCTT ATCGGAAAGA ACTAGAGAAA AACAGAAGCC ACCTTCTCCA GCAGAACCAG 
AGGCTGGATG CCTCACAGAG GCGAGTTTTA GAACTGGAAT CTCTTCTGGC CAAGAAAGAC 
CACCTTCTCC TAGAGCAGAA GAAGTACCTT GAGGATGTCA AGAGCCAGGC GAGTGGACAG 
CTGCTGGCTG CAGAGAGCAG GTATGAGGCT CAGAGAAAGA TCACCCGGGT GTTGGAACTG 
GAGATCCTAG ACTTGTATGG TAGATTGGAG AAAGATGGCC GCCTACAGAA ACTAGAAGAG 
GACAGAGCAG AGGCAGCAGA GGCAGCTGAA GAGAGGCTTG ACTGTTGTAC TGATGGATGC 
TCAGATTCCT TGTTAGGACA TAATGAAGAG GCTGCTGGTC ACAATGGTGA GACCAGGACC 
TCCAGACCTG GTGGCACCCG GGCCAGCTGT GGAGGTAGAG TCACTGGAGG CAGCAGCAGC 
AGCAGCAGTG AGCTTTCCAC TCCAGAGAAA CCCCCGAACC AGAGGTTCAG CAGCCGGTGG 
GAGCCCACCA TGGGTGAGCC CTCCAGCAGC ATCCCCACCA CTGTTGGCTC ACTTCCCAGT 
TCCAAAAGCT TCTTGGGCAT GAAGACCCGG GAGCTGTTCC GTAATAAGAG TGAGAGCCAG 
TGTGATGAGG ATGGCATGAC CATGAGTAGC TTTTCTGAGA CCCTGAAGAC AGAACTGGGC 
AAAGACTCGG CGGGCATGGA AAACAAGACT CCCCCAAGTC TAGACGCCCC ACACCCATCT 
TCCCCAAGCT CAGACAGTAT GGGGCAGCTC CATATCATGG ACTACAATGA GACTCATCAC 
GAACACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc1 ( XM_006233849.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233911.1 Rattus norvegicus tuberous sclerosis 1 (Tsc1), transcript variant X6, mRNA.

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CloneID	ORa25662
Clone ID Related Accession (Same CDS sequence)	XM_006233849.2
Accession Version	XM_006233849.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	hamartin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006233849.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC TAGCCAACAT TGGGGAGCTA CTCTCCATGC TGGACTCTTC CACACTGGGT 
GTGCGTGATG ACGTGACAAC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG 
CTTGTAAACA CGTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT CTTGCACATC 
CTGACCACCC TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAGTATGTA 
GGCAAAGCTG CCACCCGCTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTACT GAAATGTCTC 
AAGATGGACA CTGATGTTGT GGTTCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTA 
CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT 
CTTTCATCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTACCT GGTCCACCTC 
CATGCCAGTG TTTATGCCCT CTTCCATCGC CTTTATGGAA TGTACCCTTG TAACTTCGTC 
TCCTTCCTGC GCTCTCACTA CAGCATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCTAC CGAAGCCTCA TATGAAGATG GCGATGCTGT GTCTCACCAG 
CTCTCTGCCT GCTTCCCTCA CCGTTCAGCT GATGTCACCA CCAGCTCTTA CGTGGACACA 
CAGAATAGCT ATGGGGGCGC TACTTCCACC CCTTCCTCCA CCTCTCGGCT GATGTTGTTC 
AGTACCCCCG GGCAGCTACC TCAGAGTTTG AGTTCACTAT CAACACGGCC GTTACCTGAG 
CCGCTGCAAG CTAGTCTTTG GAGCCCATCT GCAGTCTGTG GTATGACCAC TCCTCCTACG 
TCTCCTGGAA ATGTCCCGGC TGATTTGTCA CATCCATACA GTAAAGCCTT TGGTACTACC 
ACTGGTGGAA AAGGAACTCC TTCAGGAACC CCAGCGACCT CTCCTCCCCC AGCCCCGCCT 
TGTCCCCAAG ACGACTGTGC GCACGGTCCA GCCTCCCAGG CCTCAGCCAC ACCCCCCAGG 
AAGGAAGAAA GAGCAGATTC TTCAAGGCCT TACCTACCCA GACAGCAGGA TGTTCCGAGT 
GACCGCGGAT TAGAGGATCT ACCTGGAAGC AAAGGTTCCG TCACTCTGAG GAATCTACCA 
GATTTCCTAG GTGACCTGGC TTCAGAGGAA GACAGTATAG AGAAAGATAA GGAAGAAGCT 
GCGATATCTA AAGAGCTTTC TGAGATCACC ACTGCAGAGG CGGATCCTGT AGCTCCTCGA 
GGGGGCTTTG ACTCTCCCTT CTACCGAGAC AGTCTCTCTG GTTCTCAGCG GAAGACTCAC 
TCAGCAGCCT CTGGGACTCA GGGCTTCAGC GTGAACCCTG AGCCTTTGCA CTCCTCCCTG 
GACAAACATG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCC ACCCTCTGGC 
AGTGCTGATG CCAGTCCCGC TGGGGACAGG GATCGCCAGA CTTCTCTGGA GACCAGTATC 
CTCACTCCCA GCCCTTGCAA AATTCCACCT CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG 
CTTCCCCCAT ATGATCATCT CTTTGAGGTG GCCTTGCCAA AGACTGCCTG TCACTTTGTC 
AGCAAAAAGA CTGAAGAGCT GTTGAAGAAA GCGAAAGGAA ACCCTGAGGA AGACTGTGTG 
CCCTCTACCT CCCCAATGGA AGTACTGGAC AGACTGCTAG AGCAGGGAGC AGGGGCACAC 
AGCAAGGAAC TGAGCAGGTT GTCCTTGCCC AGCAAGTCTG TTGACTGGAC CCACTTTGGA 
GATGAGATCC GGACCCTCCG AGACCAGTTG CTCTTACTGC ACAATCAGCT GCTGTACGAA 
CGCTTTAAGC GGCAGCAGCA TGCACTGAGG AACAGAAGGC TGCTGCGCAA GGTGATCCGA 
GCTGCGGCCC TGGAGGAGCA CAACGCAGCG ATGAAAGATC AGTTGAAGTT ACAAGAGAAG 
GACATCCAGA TGTGGAAGGT GAGTCTGCAG AAAGAACAAG CCCGATACAG TCAGCTTCAG 
CAACAACGTG ACACCATGGT GACCCAGCTG CATAGCCAAA TCAGACAGCT GCAGCATGAC 
CGAGAAGAAT TCTACAACCA GAGTCAGGAG TTACAGACGA AGCTGGAGGA CTGCAGAAGC 
ATGATCGCGG AGCTTCGAGT GGAGCTGAAG AAGGCCAACA GCAAGGTGTG CCACACCGAG 
CTGCTGCTCA GCCAGGTCTC TCAGAAGCTC TCCAATAGTG AGTCAGTGCA GCAGCAGATG 
GAGTTCTTGA ATAGGCAGCT TCTGGTGCTC GGGGAGGTCA ATGAGCTGTA CCTGGAGCAG 
CTGCAGAGCA AGCATCCTGA CACCACCAAG GAAGTAGAAA TGATGAAAAC TGCTTATCGG 
AAAGAACTAG AGAAAAACAG AAGCCACCTT CTCCAGCAGA ACCAGAGGCT GGATGCCTCA 
CAGAGGCGAG TTTTAGAACT GGAATCTCTT CTGGCCAAGA AAGACCACCT TCTCCTAGAG 
CAGAAGAAGT ACCTTGAGGA TGTCAAGAGC CAGGCGAGTG GACAGCTGCT GGCTGCAGAG 
AGCAGGTATG AGGCTCAGAG AAAGATCACC CGGGTGTTGG AACTGGAGAT CCTAGACTTG 
TATGGTAGAT TGGAGAAAGA TGGCCGCCTA CAGAAACTAG AAGAGGACAG AGCAGAGGCA 
GCAGAGGCAG CTGAAGAGAG GCTTGACTGT TGTACTGATG GATGCTCAGA TTCCTTGTTA 
GGACATAATG AAGAGGCTGC TGGTCACAAT GGTGAGACCA GGACCTCCAG ACCTGGTGGC 
ACCCGGGCCA GCTGTGGAGG TAGAGTCACT GGAGGCAGCA GCAGCAGCAG CAGTGAGCTT 
TCCACTCCAG AGAAACCCCC GAACCAGAGG TTCAGCAGCC GGTGGGAGCC CACCATGGGT 
GAGCCCTCCA GCAGCATCCC CACCACTGTT GGCTCACTTC CCAGTTCCAA AAGCTTCTTG 
GGCATGAAGA CCCGGGAGCT GTTCCGTAAT AAGAGTGAGA GCCAGTGTGA TGAGGATGGC 
ATGACCATGA GTAGCTTTTC TGAGACCCTG AAGACAGAAC TGGGCAAAGA CTCGGCGGGC 
ATGGAAAACA AGACTCCCCC AAGTCTAGAC GCCCCACACC CATCTTCCCC AAGCTCAGAC 
AGTATGGGGC AGCTCCATAT CATGGACTAC AATGAGACTC ATCACGAACA CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233911.1
CDS	455..3931
Translation	MAQLANIGELLSMLDSSTLGVRDDVTTIFKESLNSERGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAATRLSILSLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVVVLTTGVLVLITMLPMIPQSGKQHLLDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENVETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKTLETHDVVIECAKISLDPTEASYEDGDAVSHQLSACFPHRSADVTTSSYVDTQNSYGGATSTPSSTSRLMLFSTPGQLPQSLSSLSTRPLPEPLQASLWSPSAVCGMTTPPTSPGNVPADLSHPYSKAFGTTTGGKGTPSGTPATSPPPAPPCPQDDCAHGPASQASATPPRKEERADSSRPYLPRQQDVPSDRGLEDLPGSKGSVTLRNLPDFLGDLASEEDSIEKDKEEAAISKELSEITTAEADPVAPRGGFDSPFYRDSLSGSQRKTHSAASGTQGFSVNPEPLHSSLDKHGPDTPKQAFTPIDPPSGSADASPAGDRDRQTSLETSILTPSPCKIPPQRGVSFGSGQLPPYDHLFEVALPKTACHFVSKKTEELLKKAKGNPEEDCVPSTSPMEVLDRLLEQGAGAHSKELSRLSLPSKSVDWTHFGDEIRTLRDQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRAAALEEHNAAMKDQLKLQEKDIQMWKVSLQKEQARYSQLQQQRDTMVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRSMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQSKHPDTTKEVEMMKTAYRKELEKNRSHLLQQNQRLDASQRRVLELESLLAKKDHLLLEQKKYLEDVKSQASGQLLAAESRYEAQRKITRVLELEILDLYGRLEKDGRLQKLEEDRAEAAEAAEERLDCCTDGCSDSLLGHNEEAAGHNGETRTSRPGGTRASCGGRVTGGSSSSSSELSTPEKPPNQRFSSRWEPTMGEPSSSIPTTVGSLPSSKSFLGMKTRELFRNKSESQCDEDGMTMSSFSETLKTELGKDSAGMENKTPPSLDAPHPSSPSSDSMGQLHIMDYNETHHEHS

Target ORF information:

RefSeq Version	XM_006233849.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tuberous sclerosis 1 (Tsc1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233849.2

ORF Insert Sequence:

ATGGCCCAGC TAGCCAACAT TGGGGAGCTA CTCTCCATGC TGGACTCTTC CACACTGGGT 
GTGCGTGATG ACGTGACAAC CATCTTCAAG GAGTCCCTCA ATTCTGAACG TGGGCCTATG 
CTTGTAAACA CGTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT CTTGCACATC 
CTGACCACCC TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAGTATGTA 
GGCAAAGCTG CCACCCGCTT ATCCATCCTC TCGCTGCTGG GGCATGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTCTGC CTTCTTTACT GAAATGTCTC 
AAGATGGACA CTGATGTTGT GGTTCTCACA ACTGGTGTCT TGGTGTTGAT CACCATGCTA 
CCGATGATCC CGCAGTCAGG GAAGCAGCAC CTTCTCGACT TCTTTGACAT CTTTGGCCGT 
CTTTCATCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTACCT GGTCCACCTC 
CATGCCAGTG TTTATGCCCT CTTCCATCGC CTTTATGGAA TGTACCCTTG TAACTTCGTC 
TCCTTCCTGC GCTCTCACTA CAGCATGAAG GAAAACGTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGACATTA GAAACTCATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCTAC CGAAGCCTCA TATGAAGATG GCGATGCTGT GTCTCACCAG 
CTCTCTGCCT GCTTCCCTCA CCGTTCAGCT GATGTCACCA CCAGCTCTTA CGTGGACACA 
CAGAATAGCT ATGGGGGCGC TACTTCCACC CCTTCCTCCA CCTCTCGGCT GATGTTGTTC 
AGTACCCCCG GGCAGCTACC TCAGAGTTTG AGTTCACTAT CAACACGGCC GTTACCTGAG 
CCGCTGCAAG CTAGTCTTTG GAGCCCATCT GCAGTCTGTG GTATGACCAC TCCTCCTACG 
TCTCCTGGAA ATGTCCCGGC TGATTTGTCA CATCCATACA GTAAAGCCTT TGGTACTACC 
ACTGGTGGAA AAGGAACTCC TTCAGGAACC CCAGCGACCT CTCCTCCCCC AGCCCCGCCT 
TGTCCCCAAG ACGACTGTGC GCACGGTCCA GCCTCCCAGG CCTCAGCCAC ACCCCCCAGG 
AAGGAAGAAA GAGCAGATTC TTCAAGGCCT TACCTACCCA GACAGCAGGA TGTTCCGAGT 
GACCGCGGAT TAGAGGATCT ACCTGGAAGC AAAGGTTCCG TCACTCTGAG GAATCTACCA 
GATTTCCTAG GTGACCTGGC TTCAGAGGAA GACAGTATAG AGAAAGATAA GGAAGAAGCT 
GCGATATCTA AAGAGCTTTC TGAGATCACC ACTGCAGAGG CGGATCCTGT AGCTCCTCGA 
GGGGGCTTTG ACTCTCCCTT CTACCGAGAC AGTCTCTCTG GTTCTCAGCG GAAGACTCAC 
TCAGCAGCCT CTGGGACTCA GGGCTTCAGC GTGAACCCTG AGCCTTTGCA CTCCTCCCTG 
GACAAACATG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCC ACCCTCTGGC 
AGTGCTGATG CCAGTCCCGC TGGGGACAGG GATCGCCAGA CTTCTCTGGA GACCAGTATC 
CTCACTCCCA GCCCTTGCAA AATTCCACCT CAGAGGGGAG TGAGCTTTGG AAGTGGGCAG 
CTTCCCCCAT ATGATCATCT CTTTGAGGTG GCCTTGCCAA AGACTGCCTG TCACTTTGTC 
AGCAAAAAGA CTGAAGAGCT GTTGAAGAAA GCGAAAGGAA ACCCTGAGGA AGACTGTGTG 
CCCTCTACCT CCCCAATGGA AGTACTGGAC AGACTGCTAG AGCAGGGAGC AGGGGCACAC 
AGCAAGGAAC TGAGCAGGTT GTCCTTGCCC AGCAAGTCTG TTGACTGGAC CCACTTTGGA 
GATGAGATCC GGACCCTCCG AGACCAGTTG CTCTTACTGC ACAATCAGCT GCTGTACGAA 
CGCTTTAAGC GGCAGCAGCA TGCACTGAGG AACAGAAGGC TGCTGCGCAA GGTGATCCGA 
GCTGCGGCCC TGGAGGAGCA CAACGCAGCG ATGAAAGATC AGTTGAAGTT ACAAGAGAAG 
GACATCCAGA TGTGGAAGGT GAGTCTGCAG AAAGAACAAG CCCGATACAG TCAGCTTCAG 
CAACAACGTG ACACCATGGT GACCCAGCTG CATAGCCAAA TCAGACAGCT GCAGCATGAC 
CGAGAAGAAT TCTACAACCA GAGTCAGGAG TTACAGACGA AGCTGGAGGA CTGCAGAAGC 
ATGATCGCGG AGCTTCGAGT GGAGCTGAAG AAGGCCAACA GCAAGGTGTG CCACACCGAG 
CTGCTGCTCA GCCAGGTCTC TCAGAAGCTC TCCAATAGTG AGTCAGTGCA GCAGCAGATG 
GAGTTCTTGA ATAGGCAGCT TCTGGTGCTC GGGGAGGTCA ATGAGCTGTA CCTGGAGCAG 
CTGCAGAGCA AGCATCCTGA CACCACCAAG GAAGTAGAAA TGATGAAAAC TGCTTATCGG 
AAAGAACTAG AGAAAAACAG AAGCCACCTT CTCCAGCAGA ACCAGAGGCT GGATGCCTCA 
CAGAGGCGAG TTTTAGAACT GGAATCTCTT CTGGCCAAGA AAGACCACCT TCTCCTAGAG 
CAGAAGAAGT ACCTTGAGGA TGTCAAGAGC CAGGCGAGTG GACAGCTGCT GGCTGCAGAG 
AGCAGGTATG AGGCTCAGAG AAAGATCACC CGGGTGTTGG AACTGGAGAT CCTAGACTTG 
TATGGTAGAT TGGAGAAAGA TGGCCGCCTA CAGAAACTAG AAGAGGACAG AGCAGAGGCA 
GCAGAGGCAG CTGAAGAGAG GCTTGACTGT TGTACTGATG GATGCTCAGA TTCCTTGTTA 
GGACATAATG AAGAGGCTGC TGGTCACAAT GGTGAGACCA GGACCTCCAG ACCTGGTGGC 
ACCCGGGCCA GCTGTGGAGG TAGAGTCACT GGAGGCAGCA GCAGCAGCAG CAGTGAGCTT 
TCCACTCCAG AGAAACCCCC GAACCAGAGG TTCAGCAGCC GGTGGGAGCC CACCATGGGT 
GAGCCCTCCA GCAGCATCCC CACCACTGTT GGCTCACTTC CCAGTTCCAA AAGCTTCTTG 
GGCATGAAGA CCCGGGAGCT GTTCCGTAAT AAGAGTGAGA GCCAGTGTGA TGAGGATGGC 
ATGACCATGA GTAGCTTTTC TGAGACCCTG AAGACAGAAC TGGGCAAAGA CTCGGCGGGC 
ATGGAAAACA AGACTCCCCC AAGTCTAGAC GCCCCACACC CATCTTCCCC AAGCTCAGAC 
AGTATGGGGC AGCTCCATAT CATGGACTAC AATGAGACTC ATCACGAACA CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tumor suppressor Tsc1 is a new Hsp90 co-chaperone that facilitates folding of kinase and non-kinase clients.
The EMBO journal36(24)3650-3665(2017 12)
Woodford MR,Sager RA,Marris E,Dunn DM,Blanden AR,Murphy RL,Rensing N,Shapiro O,Panaretou B,Prodromou C,Loh SN,Gutmann DH,Bourboulia D,Bratslavsky G,Wong M,Mollapour M

Somatic Mutations in TSC1 and TSC2 Cause Focal Cortical Dysplasia.
American journal of human genetics100(3)454-472(2017 Mar)
Lim JS,Gopalappa R,Kim SH,Ramakrishna S,Lee M,Kim WI,Kim J,Park SM,Lee J,Oh JH,Kim HD,Park CH,Lee JS,Kim S,Kim DS,Han JM,Kang HC,Kim HH,Lee JH

Intellectual ability in tuberous sclerosis complex correlates with predicted effects of mutations on TSC1 and TSC2 proteins.
Journal of medical genetics52(12)815-22(2015 Dec)
Wong HT,McCartney DL,Lewis JC,Sampson JR,Howe CJ,de Vries PJ

Survival benefit and phenotypic improvement by hamartin gene therapy in a tuberous sclerosis mouse brain model.
Neurobiology of disease8222-31(2015 Oct)
Prabhakar S,Zhang X,Goto J,Han S,Lai C,Bronson R,Sena-Esteves M,Ramesh V,Stemmer-Rachamimov A,Kwiatkowski DJ,Breakefield XO

Regulation of mTORC1 by PI3K signaling.
Trends in cell biology25(9)545-55(2015 Sep)
Dibble CC,Cantley LC

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Tsc1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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