TSC1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015473685.1	XP_015329171.1	hamartin isoform X1
XM_005213451.3	XP_005213508.1	hamartin isoform X1
XM_010818988.2	XP_010817290.1	hamartin isoform X1
XM_015473685.2	XP_015329171.1	hamartin isoform X1
XM_010810528.3	XP_010808830.1	hamartin isoform X2
XM_010810529.3	XP_010808831.1	hamartin isoform X3
XM_005213449.3	XP_005213506.1	hamartin isoform X1
XM_005194031.3	XP_005194088.1	hamartin isoform X1
XM_015465522.1	XP_015321008.1	hamartin isoform X1
XM_010818998.2	XP_010817300.1	hamartin isoform X4
XM_005213450.4	XP_005213507.1	hamartin isoform X1
XM_005213449.4	XP_005213506.1	hamartin isoform X1
XM_024999614.1	XP_024855382.1	hamartin isoform X5
XM_024999615.1	XP_024855383.1	hamartin isoform X6
XM_015473686.1	XP_015329172.1	hamartin isoform X1
XM_010810528.2	XP_010808830.1	hamartin isoform X2
XM_010810529.2	XP_010808831.1	hamartin isoform X3
XM_010810530.2	XP_010808832.1	hamartin isoform X4
XM_010810531.2	XP_010808833.1	hamartin isoform X5
XM_005213450.3	XP_005213507.1	hamartin isoform X1
XM_005194032.3	XP_005194089.1	hamartin isoform X1
XM_015465521.1	XP_015321007.1	hamartin isoform X1
XM_005194033.3	XP_005194090.1	hamartin isoform X1
XM_010818993.2	XP_010817295.1	hamartin isoform X2
XM_024999613.1	XP_024855381.1	hamartin isoform X2
XM_010818996.2	XP_010817298.1	hamartin isoform X3
XM_010819002.2	XP_010817304.1	hamartin isoform X5
XM_010810527.2	XP_010808829.1	hamartin isoform X1
XM_010810530.3	XP_010808832.1	hamartin isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa220959	XM_024999614.1 Latest version!	Bos taurus TSC complex subunit 1 (TSC1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa07129	XM_005194031.3 Latest version!	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa07129	XM_005213451.3 Latest version!	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa07129	XM_005194032.3 Latest version!	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa07129	XM_005194033.3 Latest version!	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa07129	XM_005213450.4 Latest version!	Bos taurus TSC complex subunit 1 (TSC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa07129	XM_010818988.2 Latest version!	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa07129	XM_015473685.2 Latest version!	Bos taurus TSC complex subunit 1 (TSC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa07129	XM_005213449.4 Latest version!	Bos taurus TSC complex subunit 1 (TSC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa07129	XM_015473686.1 Latest version!	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa07129	XM_015465522.1 Latest version!	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa07129	XM_015465521.1 Latest version!	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa07129	XM_010810527.2 Latest version!	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa07130	XM_024999613.1 Latest version!	Bos taurus TSC complex subunit 1 (TSC1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa07130	XM_010818993.2 Latest version!	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa07130	XM_010810528.3 Latest version!	Bos taurus TSC complex subunit 1 (TSC1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa52450	XM_010810529.3 Latest version!	Bos taurus TSC complex subunit 1 (TSC1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa52450	XM_010818996.2 Latest version!	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa52451	XM_010810530.3 Latest version!	Bos taurus TSC complex subunit 1 (TSC1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa52451	XM_010818998.2 Latest version!	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa52452	XM_010819002.2 Latest version!	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa52452	XM_024999615.1 Latest version!	Bos taurus TSC complex subunit 1 (TSC1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa52452	XM_010810531.2 Latest version!	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
View Old Accession Versions >>
OBa07129	XM_015473685.1	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa07129	XM_005213449.3	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa07130	XM_010810528.2	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa52450	XM_010810529.2	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa07129	XM_005213450.3	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa52451	XM_010810530.2	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
Hide Old Accession Versions >>

TSC1 ( XM_024999614.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855382.1 Bos taurus TSC complex subunit 1 (TSC1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa220959
Clone ID Related Accession (Same CDS sequence)	XM_024999614.1
Accession Version	XM_024999614.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	hamartin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCAC GCCTCTGTGG GCCACTCCAA GTAGATGGGA GTTTATCCAG AGGCCAGGGG 
AGCCACCCAT GGATCTCAGA CAGTGGTGTC TGGTATGTTC AGATTTGGGT TTTAGAAAAC 
TGCTCTCCTT TACTTACTGT CTTCGGGGCA AGGCGGGGCA CACATACGCA GAAAGTCCCA 
TTGGCAGCCT GTGTGCGTGT CTGTCTTGCA CACGTGTCCC GTGTCCAGCT AGGACCCCCC 
CGGTGGATTT CTTTTTTAGG CCATGTGACA GAAGTCTATC TCGTCCACCT CCACGCCAGC 
GTCTACGCTC TCTTCCATCG CCTGTATGGG ATGTACCCTT GCAACTTTGT CTCCTTTCTG 
CGTTCTCACT ACAGTATGAA GGAAAACCTG GAGACTTTTG AAGAAGTGGT CAAGCCAATG 
ATGGAGCATG TGCGAATTCA CCCGGAATTA GTGACTGGAT CCAAGGACCA TGAACTGGAC 
CCTCGAAGGT GGAAGAGATT AGAAACACAT GATGTTGTCA TAGAATGTGC CAAAATCTCT 
CTGGACCCTA CAGAGGCCTC ATATGAAGAT GGCTATTCTG TGTCTCACCA AATCTCAGCC 
CGTTTTCCTC ACCGTTCAGC CGATGTCTCC GCCAGCTCTT ACGTTGACAC ACAGAATAGC 
TATGGGAATG CTACTTCTAC CCCCTACTCT ACTTCTCGGC TGACGTTAAA CACGCCAGGG 
CAGCTACCTC AGACTCTGAG TTCCCCGTCG ACACGGCTAT TAGCTGAGCC GCCACAAGCT 
ACTCTTTGGA GCCCATCTGC AGTTTGTGGG ATGACCACTC CTCCAACTTC TCCTGGAAAT 
GTCCCACCTG ATCTGTCACA TCCTTACAGT AAAGTCTTTG GTACAACTGG TGGGAAAGGA 
ACTCCTCTGG GAACCCCTGC AACCTCTCCT CCTCCAGCCC CACCCTGCCA TTCGGACGAC 
TGCGTGCACC TTTCACTCCC ACAGGCCACG GCCCCCAAGA AGGAAGAGAG GACAGACTCC 
GCAAGGCCCT GTCTACACAG ACAACCCCAT GTTCCGAATG ACAGAGGATT AGAAGAGCTG 
CACGGAGGCA AGGGATCTGT CACTCTCAGT GATCTTCCAG GGTTTTTAGG AGACCTGGCT 
TCTGAAGAAG ACAGTATTGA GAAGGATAAA GAAGAAGCGG CCATATCTAA AGAGCTGTCT 
GAGATCACGA CGGCCGATGC CGAGCCCCTG GTTCCTCGAG GAGGCTTCGA CTCACCCTTC 
TACCGGGACA GTCTGCCGGG CTGCCCGCGG AAGACCCTCT CAGCTTCCTC CAGCACTCAG 
GGCACCAGCG CGAACCCTGA GCCTTTAAAC TCCTCCCTGG ACAAGTCGGG GCCTGACACA 
CCAAAGCAAG CCTTCACGCC CATAGACCTG CCCTGCGGAG GCACCGGGGA GAGCCCTGCC 
GGGAACAGGG AGCGCCAGGC CTCTCTGGAG GCCAGCATCC TCACCCCCAG CCCCTGTAAA 
ATTCCACCTC AGAGGGGAGT GGGGTTTGGC AGTGGGCAGC CTCCCCCATA CGATCATCTT 
TTTGAGGTGG CATTGCCAAA GACTGCCCAT CATTTTGTCA GCAAGAAGAC GGAGGAACTG 
CTCAAGAAGG CAAAGGGAAG TGCAGAGGAG GACTTCACGC CCTCCGCCTC CCCTATGGAA 
GTGCTGGACA GGCTGATACA GCAGGGAGCG GACGTGCACA GCAAGGAGCT CAACAGGTTG 
TCTTTACCCA GCAAGTCTGT CGACTGGACC CACTTCGGAG GCTCTCCTCC CTCAGATGAG 
ATCTGCACCC TCCGTAACCA GTTGCTTTTA CTGCACAACC AGTTACTCTA CGAGCGTTTC 
AAGCGGCAGC AGCACGCGCT GCGCAACAGG CGGCTCCTGC GCAAGGTGAT CCGGGCCTCG 
GCTCTGGAGG AGCACAACGC CGCCATGAAA GACCAGTTGA AGTTGCAAGA GAAAGACATC 
CAGATATGGA AGATTAGTCT GCAGAAAGAA CAAGCCAGGT ACAATCAGCT TCAGGAGCAG 
CGAGACACAG TGGTCACCCA GCTTCATAGC CAGATCAGAC AGCTTCAGCA CGACCGGGAG 
GAGTTCTACA ACCAGAGCCA GGAGTTACAG ACGAAGCTGG AGGACTGCAG GAACATGATC 
GCAGAGCTGC GCGTGGAGCT GAAGAAGGCC AACAGCAAGG TGTGCCACAC CGAGCTGCTG 
CTCAGCCAGG TGTCCCAGAA GCTCTCAAAT AGTGAGTCGG TCCAACAGCA GATGGAGTTC 
TTGAACAGGC AACTGTTGGT TCTTGGGGAA GTCAACGAGC TGTATTTGGA ACAGCTGCAG 
AACAAGCATT CAGACACCAC CAAGGAAGTA GAAATGATGA AGGCTGCATA TCGGAAAGAG 
CTAGAAAAAA ACAGAAGCCA CGTCCTCCAG CAGAATCAGA GGCTTGATAC CTGTCAGAAG 
CGGATTTTGG AACTGGAATC TCATCTGGCT AAGAAAGACC ACCTTCTTTT AGAACAGAAA 
AAGTATCTAG AGGATGTCAA ACTGCAGGCA AGAGGACAGC TACAGGCTGC AGAGAGCAGG 
TATGAGGCCC AAAAGAGGAT CACCCAGGTG TTTGAATTGG AGATCTTAGA TTTATACGGC 
AAGTTGGAGA AGGATGGCCT CCTGAAAAAA CTTGAAGAAC AAAAACCAGA AGCAGCTGAA 
GCAGCAGAAG AAAGGCTTGA CTACTGTCAG GACGGCTGCT CGGATTCGGT GACAGGGCAC 
AACGAAGAGG CCCCTGGCCG TAACGGTGAG ACCAAGCCCT CCAGGCCTGG CGGCGCCCGG 
GGCGGTGGCG GTGGCGGCGG CAGAGGTGGC GGCAGCAGCA GCGAGCTCTC CACCCCGGAG 
AAGCCCCCCA ACCCCAGGGC GGGCCCGTTC AGCAGTCGGT GGGAGACGGT GGTGGGTGAG 
CCCACTTCCG GCACCCCCAC GACGGTGGGC TCGCTTCCCA GCTCAGAGAG CTTCCTGGGC 
ATGAAGGCAC GAGAGCTGTT TCGTAACAAG AGCGAGAGCC AATGCGACGA GGATGGCCTG 
ACTGTTGGCA GCCTTTCTGA GACCCTAAAG ACAGAGCTGG GCAAAGACTC GGGTGTGGAA 
GCCAGGGCCC CCCTGACCCT TGATGGCCCA CTGCTGCCTG CCCCAAAGCC GGACAGCGTG 
GGCCAGCTCC GCATCATGGA CTACAACGAG GCCCAGCTTG AGCACAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855382.1
CDS	3567..6797
Translation	MSARLCGPLQVDGSLSRGQGSHPWISDSGVWYVQIWVLENCSPLLTVFGARRGTHTQKVPLAACVRVCLAHVSRVQLGPPRWISFLGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGGSPPSDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_024999614.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TSC complex subunit 1 (TSC1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999614.1

ORF Insert Sequence:

ATGAGTGCAC GCCTCTGTGG GCCACTCCAA GTAGATGGGA GTTTATCCAG AGGCCAGGGG 
AGCCACCCAT GGATCTCAGA CAGTGGTGTC TGGTATGTTC AGATTTGGGT TTTAGAAAAC 
TGCTCTCCTT TACTTACTGT CTTCGGGGCA AGGCGGGGCA CACATACGCA GAAAGTCCCA 
TTGGCAGCCT GTGTGCGTGT CTGTCTTGCA CACGTGTCCC GTGTCCAGCT AGGACCCCCC 
CGGTGGATTT CTTTTTTAGG CCATGTGACA GAAGTCTATC TCGTCCACCT CCACGCCAGC 
GTCTACGCTC TCTTCCATCG CCTGTATGGG ATGTACCCTT GCAACTTTGT CTCCTTTCTG 
CGTTCTCACT ACAGTATGAA GGAAAACCTG GAGACTTTTG AAGAAGTGGT CAAGCCAATG 
ATGGAGCATG TGCGAATTCA CCCGGAATTA GTGACTGGAT CCAAGGACCA TGAACTGGAC 
CCTCGAAGGT GGAAGAGATT AGAAACACAT GATGTTGTCA TAGAATGTGC CAAAATCTCT 
CTGGACCCTA CAGAGGCCTC ATATGAAGAT GGCTATTCTG TGTCTCACCA AATCTCAGCC 
CGTTTTCCTC ACCGTTCAGC CGATGTCTCC GCCAGCTCTT ACGTTGACAC ACAGAATAGC 
TATGGGAATG CTACTTCTAC CCCCTACTCT ACTTCTCGGC TGACGTTAAA CACGCCAGGG 
CAGCTACCTC AGACTCTGAG TTCCCCGTCG ACACGGCTAT TAGCTGAGCC GCCACAAGCT 
ACTCTTTGGA GCCCATCTGC AGTTTGTGGG ATGACCACTC CTCCAACTTC TCCTGGAAAT 
GTCCCACCTG ATCTGTCACA TCCTTACAGT AAAGTCTTTG GTACAACTGG TGGGAAAGGA 
ACTCCTCTGG GAACCCCTGC AACCTCTCCT CCTCCAGCCC CACCCTGCCA TTCGGACGAC 
TGCGTGCACC TTTCACTCCC ACAGGCCACG GCCCCCAAGA AGGAAGAGAG GACAGACTCC 
GCAAGGCCCT GTCTACACAG ACAACCCCAT GTTCCGAATG ACAGAGGATT AGAAGAGCTG 
CACGGAGGCA AGGGATCTGT CACTCTCAGT GATCTTCCAG GGTTTTTAGG AGACCTGGCT 
TCTGAAGAAG ACAGTATTGA GAAGGATAAA GAAGAAGCGG CCATATCTAA AGAGCTGTCT 
GAGATCACGA CGGCCGATGC CGAGCCCCTG GTTCCTCGAG GAGGCTTCGA CTCACCCTTC 
TACCGGGACA GTCTGCCGGG CTGCCCGCGG AAGACCCTCT CAGCTTCCTC CAGCACTCAG 
GGCACCAGCG CGAACCCTGA GCCTTTAAAC TCCTCCCTGG ACAAGTCGGG GCCTGACACA 
CCAAAGCAAG CCTTCACGCC CATAGACCTG CCCTGCGGAG GCACCGGGGA GAGCCCTGCC 
GGGAACAGGG AGCGCCAGGC CTCTCTGGAG GCCAGCATCC TCACCCCCAG CCCCTGTAAA 
ATTCCACCTC AGAGGGGAGT GGGGTTTGGC AGTGGGCAGC CTCCCCCATA CGATCATCTT 
TTTGAGGTGG CATTGCCAAA GACTGCCCAT CATTTTGTCA GCAAGAAGAC GGAGGAACTG 
CTCAAGAAGG CAAAGGGAAG TGCAGAGGAG GACTTCACGC CCTCCGCCTC CCCTATGGAA 
GTGCTGGACA GGCTGATACA GCAGGGAGCG GACGTGCACA GCAAGGAGCT CAACAGGTTG 
TCTTTACCCA GCAAGTCTGT CGACTGGACC CACTTCGGAG GCTCTCCTCC CTCAGATGAG 
ATCTGCACCC TCCGTAACCA GTTGCTTTTA CTGCACAACC AGTTACTCTA CGAGCGTTTC 
AAGCGGCAGC AGCACGCGCT GCGCAACAGG CGGCTCCTGC GCAAGGTGAT CCGGGCCTCG 
GCTCTGGAGG AGCACAACGC CGCCATGAAA GACCAGTTGA AGTTGCAAGA GAAAGACATC 
CAGATATGGA AGATTAGTCT GCAGAAAGAA CAAGCCAGGT ACAATCAGCT TCAGGAGCAG 
CGAGACACAG TGGTCACCCA GCTTCATAGC CAGATCAGAC AGCTTCAGCA CGACCGGGAG 
GAGTTCTACA ACCAGAGCCA GGAGTTACAG ACGAAGCTGG AGGACTGCAG GAACATGATC 
GCAGAGCTGC GCGTGGAGCT GAAGAAGGCC AACAGCAAGG TGTGCCACAC CGAGCTGCTG 
CTCAGCCAGG TGTCCCAGAA GCTCTCAAAT AGTGAGTCGG TCCAACAGCA GATGGAGTTC 
TTGAACAGGC AACTGTTGGT TCTTGGGGAA GTCAACGAGC TGTATTTGGA ACAGCTGCAG 
AACAAGCATT CAGACACCAC CAAGGAAGTA GAAATGATGA AGGCTGCATA TCGGAAAGAG 
CTAGAAAAAA ACAGAAGCCA CGTCCTCCAG CAGAATCAGA GGCTTGATAC CTGTCAGAAG 
CGGATTTTGG AACTGGAATC TCATCTGGCT AAGAAAGACC ACCTTCTTTT AGAACAGAAA 
AAGTATCTAG AGGATGTCAA ACTGCAGGCA AGAGGACAGC TACAGGCTGC AGAGAGCAGG 
TATGAGGCCC AAAAGAGGAT CACCCAGGTG TTTGAATTGG AGATCTTAGA TTTATACGGC 
AAGTTGGAGA AGGATGGCCT CCTGAAAAAA CTTGAAGAAC AAAAACCAGA AGCAGCTGAA 
GCAGCAGAAG AAAGGCTTGA CTACTGTCAG GACGGCTGCT CGGATTCGGT GACAGGGCAC 
AACGAAGAGG CCCCTGGCCG TAACGGTGAG ACCAAGCCCT CCAGGCCTGG CGGCGCCCGG 
GGCGGTGGCG GTGGCGGCGG CAGAGGTGGC GGCAGCAGCA GCGAGCTCTC CACCCCGGAG 
AAGCCCCCCA ACCCCAGGGC GGGCCCGTTC AGCAGTCGGT GGGAGACGGT GGTGGGTGAG 
CCCACTTCCG GCACCCCCAC GACGGTGGGC TCGCTTCCCA GCTCAGAGAG CTTCCTGGGC 
ATGAAGGCAC GAGAGCTGTT TCGTAACAAG AGCGAGAGCC AATGCGACGA GGATGGCCTG 
ACTGTTGGCA GCCTTTCTGA GACCCTAAAG ACAGAGCTGG GCAAAGACTC GGGTGTGGAA 
GCCAGGGCCC CCCTGACCCT TGATGGCCCA CTGCTGCCTG CCCCAAAGCC GGACAGCGTG 
GGCCAGCTCC GCATCATGGA CTACAACGAG GCCCAGCTTG AGCACAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_015473685.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329171.1 Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa07129
Clone ID Related Accession (Same CDS sequence)	XM_015473685.1 , XM_005213451.3 , XM_010818988.2 , XM_015473685.2 , XM_005213449.3 , XM_005194031.3 , XM_015465522.1 , XM_005213450.4 , XM_005213449.4 , XM_015473686.1 , XM_005213450.3 , XM_005194032.3 , XM_015465521.1 , XM_005194033.3 , XM_010810527.2
Accession Version	XM_015473685.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329171.1
CDS	164..3646
Translation	MAQQANIGELLSMLDSPLLSVRDDVTTVFKENLNSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSALLLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGGSPPSDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_015473685.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473685.1

ORF Insert Sequence:

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_005213451.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005213508.1 Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X6, mRNA.

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CloneID	OBa07129
Clone ID Related Accession (Same CDS sequence)	XM_015473685.1 , XM_005213451.3 , XM_010818988.2 , XM_015473685.2 , XM_005213449.3 , XM_005194031.3 , XM_015465522.1 , XM_005213450.4 , XM_005213449.4 , XM_015473686.1 , XM_005213450.3 , XM_005194032.3 , XM_015465521.1 , XM_005194033.3 , XM_010810527.2
Accession Version	XM_005213451.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213451.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005213508.1
CDS	864..4346
Translation	MAQQANIGELLSMLDSPLLSVRDDVTTVFKENLNSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSALLLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGGSPPSDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_005213451.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005213451.3

ORF Insert Sequence:

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_010818988.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817290.1 Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X5, mRNA.

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CloneID	OBa07129
Clone ID Related Accession (Same CDS sequence)	XM_015473685.1 , XM_005213451.3 , XM_010818988.2 , XM_015473685.2 , XM_005213449.3 , XM_005194031.3 , XM_015465522.1 , XM_005213450.4 , XM_005213449.4 , XM_015473686.1 , XM_005213450.3 , XM_005194032.3 , XM_015465521.1 , XM_005194033.3 , XM_010810527.2
Accession Version	XM_010818988.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818988.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817290.1
CDS	412..3894
Translation	MAQQANIGELLSMLDSPLLSVRDDVTTVFKENLNSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSALLLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGGSPPSDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_010818988.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818988.2

ORF Insert Sequence:

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_015473685.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329171.1 Bos taurus TSC complex subunit 1 (TSC1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa07129
Clone ID Related Accession (Same CDS sequence)	XM_015473685.1 , XM_005213451.3 , XM_010818988.2 , XM_015473685.2 , XM_005213449.3 , XM_005194031.3 , XM_015465522.1 , XM_005213450.4 , XM_005213449.4 , XM_015473686.1 , XM_005213450.3 , XM_005194032.3 , XM_015465521.1 , XM_005194033.3 , XM_010810527.2
Accession Version	XM_015473685.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473685.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329171.1
CDS	162..3644
Translation	MAQQANIGELLSMLDSPLLSVRDDVTTVFKENLNSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSALLLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGGSPPSDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_015473685.2
Organism	Bos taurus(cattle)
Definition	Bos taurus TSC complex subunit 1 (TSC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473685.2

ORF Insert Sequence:

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_005213449.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005213506.1 Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X2, mRNA.

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CloneID	OBa07129
Clone ID Related Accession (Same CDS sequence)	XM_015473685.1 , XM_005213451.3 , XM_010818988.2 , XM_015473685.2 , XM_005213449.3 , XM_005194031.3 , XM_015465522.1 , XM_005213450.4 , XM_005213449.4 , XM_015473686.1 , XM_005213450.3 , XM_005194032.3 , XM_015465521.1 , XM_005194033.3 , XM_010810527.2
Accession Version	XM_005213449.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213449.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005213506.1
CDS	335..3817
Translation	MAQQANIGELLSMLDSPLLSVRDDVTTVFKENLNSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSALLLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGGSPPSDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_005213449.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005213449.3

ORF Insert Sequence:

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_005194031.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005194088.1 Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa07129
Clone ID Related Accession (Same CDS sequence)	XM_015473685.1 , XM_005213451.3 , XM_010818988.2 , XM_015473685.2 , XM_005213449.3 , XM_005194031.3 , XM_015465522.1 , XM_005213450.4 , XM_005213449.4 , XM_015473686.1 , XM_005213450.3 , XM_005194032.3 , XM_015465521.1 , XM_005194033.3 , XM_010810527.2
Accession Version	XM_005194031.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005194031.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005194088.1
CDS	335..3817
Translation	MAQQANIGELLSMLDSPLLSVRDDVTTVFKENLNSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSALLLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGGSPPSDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_005194031.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005194031.3

ORF Insert Sequence:

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_015465522.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321008.1 Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa07129
Clone ID Related Accession (Same CDS sequence)	XM_015473685.1 , XM_005213451.3 , XM_010818988.2 , XM_015473685.2 , XM_005213449.3 , XM_005194031.3 , XM_015465522.1 , XM_005213450.4 , XM_005213449.4 , XM_015473686.1 , XM_005213450.3 , XM_005194032.3 , XM_015465521.1 , XM_005194033.3 , XM_010810527.2
Accession Version	XM_015465522.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321008.1
CDS	636..4118
Translation	MAQQANIGELLSMLDSPLLSVRDDVTTVFKENLNSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSALLLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGGSPPSDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_015465522.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465522.1

ORF Insert Sequence:

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_005213450.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005213507.1 Bos taurus TSC complex subunit 1 (TSC1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa07129
Clone ID Related Accession (Same CDS sequence)	XM_015473685.1 , XM_005213451.3 , XM_010818988.2 , XM_015473685.2 , XM_005213449.3 , XM_005194031.3 , XM_015465522.1 , XM_005213450.4 , XM_005213449.4 , XM_015473686.1 , XM_005213450.3 , XM_005194032.3 , XM_015465521.1 , XM_005194033.3 , XM_010810527.2
Accession Version	XM_005213450.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005213450.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005213507.1
CDS	381..3863
Translation	MAQQANIGELLSMLDSPLLSVRDDVTTVFKENLNSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSALLLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGGSPPSDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_005213450.4
Organism	Bos taurus(cattle)
Definition	Bos taurus TSC complex subunit 1 (TSC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005213450.4

ORF Insert Sequence:

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_005213449.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005213506.1 Bos taurus TSC complex subunit 1 (TSC1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa07129
Clone ID Related Accession (Same CDS sequence)	XM_015473685.1 , XM_005213451.3 , XM_010818988.2 , XM_015473685.2 , XM_005213449.3 , XM_005194031.3 , XM_015465522.1 , XM_005213450.4 , XM_005213449.4 , XM_015473686.1 , XM_005213450.3 , XM_005194032.3 , XM_015465521.1 , XM_005194033.3 , XM_010810527.2
Accession Version	XM_005213449.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005213449.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005213506.1
CDS	352..3834
Translation	MAQQANIGELLSMLDSPLLSVRDDVTTVFKENLNSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSALLLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGGSPPSDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_005213449.4
Organism	Bos taurus(cattle)
Definition	Bos taurus TSC complex subunit 1 (TSC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005213449.4

ORF Insert Sequence:

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_015473686.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329172.1 Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X4, mRNA.

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CloneID	OBa07129
Clone ID Related Accession (Same CDS sequence)	XM_015473685.1 , XM_005213451.3 , XM_010818988.2 , XM_015473685.2 , XM_005213449.3 , XM_005194031.3 , XM_015465522.1 , XM_005213450.4 , XM_005213449.4 , XM_015473686.1 , XM_005213450.3 , XM_005194032.3 , XM_015465521.1 , XM_005194033.3 , XM_010810527.2
Accession Version	XM_015473686.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329172.1
CDS	636..4118
Translation	MAQQANIGELLSMLDSPLLSVRDDVTTVFKENLNSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSALLLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGGSPPSDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_015473686.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473686.1

ORF Insert Sequence:

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_005213450.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005213507.1 Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa07129
Clone ID Related Accession (Same CDS sequence)	XM_015473685.1 , XM_005213451.3 , XM_010818988.2 , XM_015473685.2 , XM_005213449.3 , XM_005194031.3 , XM_015465522.1 , XM_005213450.4 , XM_005213449.4 , XM_015473686.1 , XM_005213450.3 , XM_005194032.3 , XM_015465521.1 , XM_005194033.3 , XM_010810527.2
Accession Version	XM_005213450.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213450.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005213507.1
CDS	241..3723
Translation	MAQQANIGELLSMLDSPLLSVRDDVTTVFKENLNSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSALLLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGGSPPSDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_005213450.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005213450.3

ORF Insert Sequence:

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_005194032.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005194089.1 Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa07129
Clone ID Related Accession (Same CDS sequence)	XM_015473685.1 , XM_005213451.3 , XM_010818988.2 , XM_015473685.2 , XM_005213449.3 , XM_005194031.3 , XM_015465522.1 , XM_005213450.4 , XM_005213449.4 , XM_015473686.1 , XM_005213450.3 , XM_005194032.3 , XM_015465521.1 , XM_005194033.3 , XM_010810527.2
Accession Version	XM_005194032.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005194032.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005194089.1
CDS	241..3723
Translation	MAQQANIGELLSMLDSPLLSVRDDVTTVFKENLNSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSALLLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGGSPPSDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_005194032.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005194032.3

ORF Insert Sequence:

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_015465521.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321007.1 Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA.

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CloneID	OBa07129
Clone ID Related Accession (Same CDS sequence)	XM_015473685.1 , XM_005213451.3 , XM_010818988.2 , XM_015473685.2 , XM_005213449.3 , XM_005194031.3 , XM_015465522.1 , XM_005213450.4 , XM_005213449.4 , XM_015473686.1 , XM_005213450.3 , XM_005194032.3 , XM_015465521.1 , XM_005194033.3 , XM_010810527.2
Accession Version	XM_015465521.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321007.1
CDS	164..3646
Translation	MAQQANIGELLSMLDSPLLSVRDDVTTVFKENLNSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSALLLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGGSPPSDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_015465521.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465521.1

ORF Insert Sequence:

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_005194033.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005194090.1 Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa07129
Clone ID Related Accession (Same CDS sequence)	XM_015473685.1 , XM_005213451.3 , XM_010818988.2 , XM_015473685.2 , XM_005213449.3 , XM_005194031.3 , XM_015465522.1 , XM_005213450.4 , XM_005213449.4 , XM_015473686.1 , XM_005213450.3 , XM_005194032.3 , XM_015465521.1 , XM_005194033.3 , XM_010810527.2
Accession Version	XM_005194033.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005194033.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005194090.1
CDS	864..4346
Translation	MAQQANIGELLSMLDSPLLSVRDDVTTVFKENLNSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSALLLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGGSPPSDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_005194033.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005194033.3

ORF Insert Sequence:

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_010810527.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808829.1 Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa07129
Clone ID Related Accession (Same CDS sequence)	XM_015473685.1 , XM_005213451.3 , XM_010818988.2 , XM_015473685.2 , XM_005213449.3 , XM_005194031.3 , XM_015465522.1 , XM_005213450.4 , XM_005213449.4 , XM_015473686.1 , XM_005213450.3 , XM_005194032.3 , XM_015465521.1 , XM_005194033.3 , XM_010810527.2
Accession Version	XM_010810527.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810527.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808829.1
CDS	412..3894
Translation	MAQQANIGELLSMLDSPLLSVRDDVTTVFKENLNSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSALLLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGGSPPSDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_010810527.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810527.2

ORF Insert Sequence:

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGGCTCTCCT 
CCCTCAGATG AGATCTGCAC CCTCCGTAAC CAGTTGCTTT TACTGCACAA CCAGTTACTC 
TACGAGCGTT TCAAGCGGCA GCAGCACGCG CTGCGCAACA GGCGGCTCCT GCGCAAGGTG 
ATCCGGGCCT CGGCTCTGGA GGAGCACAAC GCCGCCATGA AAGACCAGTT GAAGTTGCAA 
GAGAAAGACA TCCAGATATG GAAGATTAGT CTGCAGAAAG AACAAGCCAG GTACAATCAG 
CTTCAGGAGC AGCGAGACAC AGTGGTCACC CAGCTTCATA GCCAGATCAG ACAGCTTCAG 
CACGACCGGG AGGAGTTCTA CAACCAGAGC CAGGAGTTAC AGACGAAGCT GGAGGACTGC 
AGGAACATGA TCGCAGAGCT GCGCGTGGAG CTGAAGAAGG CCAACAGCAA GGTGTGCCAC 
ACCGAGCTGC TGCTCAGCCA GGTGTCCCAG AAGCTCTCAA ATAGTGAGTC GGTCCAACAG 
CAGATGGAGT TCTTGAACAG GCAACTGTTG GTTCTTGGGG AAGTCAACGA GCTGTATTTG 
GAACAGCTGC AGAACAAGCA TTCAGACACC ACCAAGGAAG TAGAAATGAT GAAGGCTGCA 
TATCGGAAAG AGCTAGAAAA AAACAGAAGC CACGTCCTCC AGCAGAATCA GAGGCTTGAT 
ACCTGTCAGA AGCGGATTTT GGAACTGGAA TCTCATCTGG CTAAGAAAGA CCACCTTCTT 
TTAGAACAGA AAAAGTATCT AGAGGATGTC AAACTGCAGG CAAGAGGACA GCTACAGGCT 
GCAGAGAGCA GGTATGAGGC CCAAAAGAGG ATCACCCAGG TGTTTGAATT GGAGATCTTA 
GATTTATACG GCAAGTTGGA GAAGGATGGC CTCCTGAAAA AACTTGAAGA ACAAAAACCA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTT GACTACTGTC AGGACGGCTG CTCGGATTCG 
GTGACAGGGC ACAACGAAGA GGCCCCTGGC CGTAACGGTG AGACCAAGCC CTCCAGGCCT 
GGCGGCGCCC GGGGCGGTGG CGGTGGCGGC GGCAGAGGTG GCGGCAGCAG CAGCGAGCTC 
TCCACCCCGG AGAAGCCCCC CAACCCCAGG GCGGGCCCGT TCAGCAGTCG GTGGGAGACG 
GTGGTGGGTG AGCCCACTTC CGGCACCCCC ACGACGGTGG GCTCGCTTCC CAGCTCAGAG 
AGCTTCCTGG GCATGAAGGC ACGAGAGCTG TTTCGTAACA AGAGCGAGAG CCAATGCGAC 
GAGGATGGCC TGACTGTTGG CAGCCTTTCT GAGACCCTAA AGACAGAGCT GGGCAAAGAC 
TCGGGTGTGG AAGCCAGGGC CCCCCTGACC CTTGATGGCC CACTGCTGCC TGCCCCAAAG 
CCGGACAGCG TGGGCCAGCT CCGCATCATG GACTACAACG AGGCCCAGCT TGAGCACAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_010810528.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808830.1 Bos taurus TSC complex subunit 1 (TSC1), transcript variant X4, mRNA.

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CloneID	OBa07130
Clone ID Related Accession (Same CDS sequence)	XM_010810528.3 , XM_010810528.2 , XM_010818993.2 , XM_024999613.1
Accession Version	XM_010810528.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	hamartin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810528.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGGT 
GGGAAAGGAA CTCCTCTGGG AACCCCTGCA ACCTCTCCTC CTCCAGCCCC ACCCTGCCAT 
TCGGACGACT GCGTGCACCT TTCACTCCCA CAGGCCACGG CCCCCAAGAA GGAAGAGAGG 
ACAGACTCCG CAAGGCCCTG TCTACACAGA CAACCCCATG TTCCGAATGA CAGAGGATTA 
GAAGAGCTGC ACGGAGGCAA GGGATCTGTC ACTCTCAGTG ATCTTCCAGG GTTTTTAGGA 
GACCTGGCTT CTGAAGAAGA CAGTATTGAG AAGGATAAAG AAGAAGCGGC CATATCTAAA 
GAGCTGTCTG AGATCACGAC GGCCGATGCC GAGCCCCTGG TTCCTCGAGG AGGCTTCGAC 
TCACCCTTCT ACCGGGACAG TCTGCCGGGC TGCCCGCGGA AGACCCTCTC AGCTTCCTCC 
AGCACTCAGG GCACCAGCGC GAACCCTGAG CCTTTAAACT CCTCCCTGGA CAAGTCGGGG 
CCTGACACAC CAAAGCAAGC CTTCACGCCC ATAGACCTGC CCTGCGGAGG CACCGGGGAG 
AGCCCTGCCG GGAACAGGGA GCGCCAGGCC TCTCTGGAGG CCAGCATCCT CACCCCCAGC 
CCCTGTAAAA TTCCACCTCA GAGGGGAGTG GGGTTTGGCA GTGGGCAGCC TCCCCCATAC 
GATCATCTTT TTGAGGTGGC ATTGCCAAAG ACTGCCCATC ATTTTGTCAG CAAGAAGACG 
GAGGAACTGC TCAAGAAGGC AAAGGGAAGT GCAGAGGAGG ACTTCACGCC CTCCGCCTCC 
CCTATGGAAG TGCTGGACAG GCTGATACAG CAGGGAGCGG ACGTGCACAG CAAGGAGCTC 
AACAGGTTGT CTTTACCCAG CAAGTCTGTC GACTGGACCC ACTTCGGAGG CTCTCCTCCC 
TCAGATGAGA TCTGCACCCT CCGTAACCAG TTGCTTTTAC TGCACAACCA GTTACTCTAC 
GAGCGTTTCA AGCGGCAGCA GCACGCGCTG CGCAACAGGC GGCTCCTGCG CAAGGTGATC 
CGGGCCTCGG CTCTGGAGGA GCACAACGCC GCCATGAAAG ACCAGTTGAA GTTGCAAGAG 
AAAGACATCC AGATATGGAA GATTAGTCTG CAGAAAGAAC AAGCCAGGTA CAATCAGCTT 
CAGGAGCAGC GAGACACAGT GGTCACCCAG CTTCATAGCC AGATCAGACA GCTTCAGCAC 
GACCGGGAGG AGTTCTACAA CCAGAGCCAG GAGTTACAGA CGAAGCTGGA GGACTGCAGG 
AACATGATCG CAGAGCTGCG CGTGGAGCTG AAGAAGGCCA ACAGCAAGGT GTGCCACACC 
GAGCTGCTGC TCAGCCAGGT GTCCCAGAAG CTCTCAAATA GTGAGTCGGT CCAACAGCAG 
ATGGAGTTCT TGAACAGGCA ACTGTTGGTT CTTGGGGAAG TCAACGAGCT GTATTTGGAA 
CAGCTGCAGA ACAAGCATTC AGACACCACC AAGGAAGTAG AAATGATGAA GGCTGCATAT 
CGGAAAGAGC TAGAAAAAAA CAGAAGCCAC GTCCTCCAGC AGAATCAGAG GCTTGATACC 
TGTCAGAAGC GGATTTTGGA ACTGGAATCT CATCTGGCTA AGAAAGACCA CCTTCTTTTA 
GAACAGAAAA AGTATCTAGA GGATGTCAAA CTGCAGGCAA GAGGACAGCT ACAGGCTGCA 
GAGAGCAGGT ATGAGGCCCA AAAGAGGATC ACCCAGGTGT TTGAATTGGA GATCTTAGAT 
TTATACGGCA AGTTGGAGAA GGATGGCCTC CTGAAAAAAC TTGAAGAACA AAAACCAGAA 
GCAGCTGAAG CAGCAGAAGA AAGGCTTGAC TACTGTCAGG ACGGCTGCTC GGATTCGGTG 
ACAGGGCACA ACGAAGAGGC CCCTGGCCGT AACGGTGAGA CCAAGCCCTC CAGGCCTGGC 
GGCGCCCGGG GCGGTGGCGG TGGCGGCGGC AGAGGTGGCG GCAGCAGCAG CGAGCTCTCC 
ACCCCGGAGA AGCCCCCCAA CCCCAGGGCG GGCCCGTTCA GCAGTCGGTG GGAGACGGTG 
GTGGGTGAGC CCACTTCCGG CACCCCCACG ACGGTGGGCT CGCTTCCCAG CTCAGAGAGC 
TTCCTGGGCA TGAAGGCACG AGAGCTGTTT CGTAACAAGA GCGAGAGCCA ATGCGACGAG 
GATGGCCTGA CTGTTGGCAG CCTTTCTGAG ACCCTAAAGA CAGAGCTGGG CAAAGACTCG 
GGTGTGGAAG CCAGGGCCCC CCTGACCCTT GATGGCCCAC TGCTGCCTGC CCCAAAGCCG 
GACAGCGTGG GCCAGCTCCG CATCATGGAC TACAACGAGG CCCAGCTTGA GCACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808830.1
CDS	251..3730
Translation	MAQQANIGELLSMLDSPLLSVRDDVTTVFKENLNSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSALLLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGGSPPSDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_010810528.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TSC complex subunit 1 (TSC1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810528.3

ORF Insert Sequence:

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGGT 
GGGAAAGGAA CTCCTCTGGG AACCCCTGCA ACCTCTCCTC CTCCAGCCCC ACCCTGCCAT 
TCGGACGACT GCGTGCACCT TTCACTCCCA CAGGCCACGG CCCCCAAGAA GGAAGAGAGG 
ACAGACTCCG CAAGGCCCTG TCTACACAGA CAACCCCATG TTCCGAATGA CAGAGGATTA 
GAAGAGCTGC ACGGAGGCAA GGGATCTGTC ACTCTCAGTG ATCTTCCAGG GTTTTTAGGA 
GACCTGGCTT CTGAAGAAGA CAGTATTGAG AAGGATAAAG AAGAAGCGGC CATATCTAAA 
GAGCTGTCTG AGATCACGAC GGCCGATGCC GAGCCCCTGG TTCCTCGAGG AGGCTTCGAC 
TCACCCTTCT ACCGGGACAG TCTGCCGGGC TGCCCGCGGA AGACCCTCTC AGCTTCCTCC 
AGCACTCAGG GCACCAGCGC GAACCCTGAG CCTTTAAACT CCTCCCTGGA CAAGTCGGGG 
CCTGACACAC CAAAGCAAGC CTTCACGCCC ATAGACCTGC CCTGCGGAGG CACCGGGGAG 
AGCCCTGCCG GGAACAGGGA GCGCCAGGCC TCTCTGGAGG CCAGCATCCT CACCCCCAGC 
CCCTGTAAAA TTCCACCTCA GAGGGGAGTG GGGTTTGGCA GTGGGCAGCC TCCCCCATAC 
GATCATCTTT TTGAGGTGGC ATTGCCAAAG ACTGCCCATC ATTTTGTCAG CAAGAAGACG 
GAGGAACTGC TCAAGAAGGC AAAGGGAAGT GCAGAGGAGG ACTTCACGCC CTCCGCCTCC 
CCTATGGAAG TGCTGGACAG GCTGATACAG CAGGGAGCGG ACGTGCACAG CAAGGAGCTC 
AACAGGTTGT CTTTACCCAG CAAGTCTGTC GACTGGACCC ACTTCGGAGG CTCTCCTCCC 
TCAGATGAGA TCTGCACCCT CCGTAACCAG TTGCTTTTAC TGCACAACCA GTTACTCTAC 
GAGCGTTTCA AGCGGCAGCA GCACGCGCTG CGCAACAGGC GGCTCCTGCG CAAGGTGATC 
CGGGCCTCGG CTCTGGAGGA GCACAACGCC GCCATGAAAG ACCAGTTGAA GTTGCAAGAG 
AAAGACATCC AGATATGGAA GATTAGTCTG CAGAAAGAAC AAGCCAGGTA CAATCAGCTT 
CAGGAGCAGC GAGACACAGT GGTCACCCAG CTTCATAGCC AGATCAGACA GCTTCAGCAC 
GACCGGGAGG AGTTCTACAA CCAGAGCCAG GAGTTACAGA CGAAGCTGGA GGACTGCAGG 
AACATGATCG CAGAGCTGCG CGTGGAGCTG AAGAAGGCCA ACAGCAAGGT GTGCCACACC 
GAGCTGCTGC TCAGCCAGGT GTCCCAGAAG CTCTCAAATA GTGAGTCGGT CCAACAGCAG 
ATGGAGTTCT TGAACAGGCA ACTGTTGGTT CTTGGGGAAG TCAACGAGCT GTATTTGGAA 
CAGCTGCAGA ACAAGCATTC AGACACCACC AAGGAAGTAG AAATGATGAA GGCTGCATAT 
CGGAAAGAGC TAGAAAAAAA CAGAAGCCAC GTCCTCCAGC AGAATCAGAG GCTTGATACC 
TGTCAGAAGC GGATTTTGGA ACTGGAATCT CATCTGGCTA AGAAAGACCA CCTTCTTTTA 
GAACAGAAAA AGTATCTAGA GGATGTCAAA CTGCAGGCAA GAGGACAGCT ACAGGCTGCA 
GAGAGCAGGT ATGAGGCCCA AAAGAGGATC ACCCAGGTGT TTGAATTGGA GATCTTAGAT 
TTATACGGCA AGTTGGAGAA GGATGGCCTC CTGAAAAAAC TTGAAGAACA AAAACCAGAA 
GCAGCTGAAG CAGCAGAAGA AAGGCTTGAC TACTGTCAGG ACGGCTGCTC GGATTCGGTG 
ACAGGGCACA ACGAAGAGGC CCCTGGCCGT AACGGTGAGA CCAAGCCCTC CAGGCCTGGC 
GGCGCCCGGG GCGGTGGCGG TGGCGGCGGC AGAGGTGGCG GCAGCAGCAG CGAGCTCTCC 
ACCCCGGAGA AGCCCCCCAA CCCCAGGGCG GGCCCGTTCA GCAGTCGGTG GGAGACGGTG 
GTGGGTGAGC CCACTTCCGG CACCCCCACG ACGGTGGGCT CGCTTCCCAG CTCAGAGAGC 
TTCCTGGGCA TGAAGGCACG AGAGCTGTTT CGTAACAAGA GCGAGAGCCA ATGCGACGAG 
GATGGCCTGA CTGTTGGCAG CCTTTCTGAG ACCCTAAAGA CAGAGCTGGG CAAAGACTCG 
GGTGTGGAAG CCAGGGCCCC CCTGACCCTT GATGGCCCAC TGCTGCCTGC CCCAAAGCCG 
GACAGCGTGG GCCAGCTCCG CATCATGGAC TACAACGAGG CCCAGCTTGA GCACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_010810528.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808830.1 Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X7, mRNA.

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CloneID	OBa07130
Clone ID Related Accession (Same CDS sequence)	XM_010810528.3 , XM_010810528.2 , XM_010818993.2 , XM_024999613.1
Accession Version	XM_010810528.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hamartin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810528.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGGT 
GGGAAAGGAA CTCCTCTGGG AACCCCTGCA ACCTCTCCTC CTCCAGCCCC ACCCTGCCAT 
TCGGACGACT GCGTGCACCT TTCACTCCCA CAGGCCACGG CCCCCAAGAA GGAAGAGAGG 
ACAGACTCCG CAAGGCCCTG TCTACACAGA CAACCCCATG TTCCGAATGA CAGAGGATTA 
GAAGAGCTGC ACGGAGGCAA GGGATCTGTC ACTCTCAGTG ATCTTCCAGG GTTTTTAGGA 
GACCTGGCTT CTGAAGAAGA CAGTATTGAG AAGGATAAAG AAGAAGCGGC CATATCTAAA 
GAGCTGTCTG AGATCACGAC GGCCGATGCC GAGCCCCTGG TTCCTCGAGG AGGCTTCGAC 
TCACCCTTCT ACCGGGACAG TCTGCCGGGC TGCCCGCGGA AGACCCTCTC AGCTTCCTCC 
AGCACTCAGG GCACCAGCGC GAACCCTGAG CCTTTAAACT CCTCCCTGGA CAAGTCGGGG 
CCTGACACAC CAAAGCAAGC CTTCACGCCC ATAGACCTGC CCTGCGGAGG CACCGGGGAG 
AGCCCTGCCG GGAACAGGGA GCGCCAGGCC TCTCTGGAGG CCAGCATCCT CACCCCCAGC 
CCCTGTAAAA TTCCACCTCA GAGGGGAGTG GGGTTTGGCA GTGGGCAGCC TCCCCCATAC 
GATCATCTTT TTGAGGTGGC ATTGCCAAAG ACTGCCCATC ATTTTGTCAG CAAGAAGACG 
GAGGAACTGC TCAAGAAGGC AAAGGGAAGT GCAGAGGAGG ACTTCACGCC CTCCGCCTCC 
CCTATGGAAG TGCTGGACAG GCTGATACAG CAGGGAGCGG ACGTGCACAG CAAGGAGCTC 
AACAGGTTGT CTTTACCCAG CAAGTCTGTC GACTGGACCC ACTTCGGAGG CTCTCCTCCC 
TCAGATGAGA TCTGCACCCT CCGTAACCAG TTGCTTTTAC TGCACAACCA GTTACTCTAC 
GAGCGTTTCA AGCGGCAGCA GCACGCGCTG CGCAACAGGC GGCTCCTGCG CAAGGTGATC 
CGGGCCTCGG CTCTGGAGGA GCACAACGCC GCCATGAAAG ACCAGTTGAA GTTGCAAGAG 
AAAGACATCC AGATATGGAA GATTAGTCTG CAGAAAGAAC AAGCCAGGTA CAATCAGCTT 
CAGGAGCAGC GAGACACAGT GGTCACCCAG CTTCATAGCC AGATCAGACA GCTTCAGCAC 
GACCGGGAGG AGTTCTACAA CCAGAGCCAG GAGTTACAGA CGAAGCTGGA GGACTGCAGG 
AACATGATCG CAGAGCTGCG CGTGGAGCTG AAGAAGGCCA ACAGCAAGGT GTGCCACACC 
GAGCTGCTGC TCAGCCAGGT GTCCCAGAAG CTCTCAAATA GTGAGTCGGT CCAACAGCAG 
ATGGAGTTCT TGAACAGGCA ACTGTTGGTT CTTGGGGAAG TCAACGAGCT GTATTTGGAA 
CAGCTGCAGA ACAAGCATTC AGACACCACC AAGGAAGTAG AAATGATGAA GGCTGCATAT 
CGGAAAGAGC TAGAAAAAAA CAGAAGCCAC GTCCTCCAGC AGAATCAGAG GCTTGATACC 
TGTCAGAAGC GGATTTTGGA ACTGGAATCT CATCTGGCTA AGAAAGACCA CCTTCTTTTA 
GAACAGAAAA AGTATCTAGA GGATGTCAAA CTGCAGGCAA GAGGACAGCT ACAGGCTGCA 
GAGAGCAGGT ATGAGGCCCA AAAGAGGATC ACCCAGGTGT TTGAATTGGA GATCTTAGAT 
TTATACGGCA AGTTGGAGAA GGATGGCCTC CTGAAAAAAC TTGAAGAACA AAAACCAGAA 
GCAGCTGAAG CAGCAGAAGA AAGGCTTGAC TACTGTCAGG ACGGCTGCTC GGATTCGGTG 
ACAGGGCACA ACGAAGAGGC CCCTGGCCGT AACGGTGAGA CCAAGCCCTC CAGGCCTGGC 
GGCGCCCGGG GCGGTGGCGG TGGCGGCGGC AGAGGTGGCG GCAGCAGCAG CGAGCTCTCC 
ACCCCGGAGA AGCCCCCCAA CCCCAGGGCG GGCCCGTTCA GCAGTCGGTG GGAGACGGTG 
GTGGGTGAGC CCACTTCCGG CACCCCCACG ACGGTGGGCT CGCTTCCCAG CTCAGAGAGC 
TTCCTGGGCA TGAAGGCACG AGAGCTGTTT CGTAACAAGA GCGAGAGCCA ATGCGACGAG 
GATGGCCTGA CTGTTGGCAG CCTTTCTGAG ACCCTAAAGA CAGAGCTGGG CAAAGACTCG 
GGTGTGGAAG CCAGGGCCCC CCTGACCCTT GATGGCCCAC TGCTGCCTGC CCCAAAGCCG 
GACAGCGTGG GCCAGCTCCG CATCATGGAC TACAACGAGG CCCAGCTTGA GCACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808830.1
CDS	241..3720
Translation	MAQQANIGELLSMLDSPLLSVRDDVTTVFKENLNSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSALLLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGGSPPSDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_010810528.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810528.2

ORF Insert Sequence:

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGGT 
GGGAAAGGAA CTCCTCTGGG AACCCCTGCA ACCTCTCCTC CTCCAGCCCC ACCCTGCCAT 
TCGGACGACT GCGTGCACCT TTCACTCCCA CAGGCCACGG CCCCCAAGAA GGAAGAGAGG 
ACAGACTCCG CAAGGCCCTG TCTACACAGA CAACCCCATG TTCCGAATGA CAGAGGATTA 
GAAGAGCTGC ACGGAGGCAA GGGATCTGTC ACTCTCAGTG ATCTTCCAGG GTTTTTAGGA 
GACCTGGCTT CTGAAGAAGA CAGTATTGAG AAGGATAAAG AAGAAGCGGC CATATCTAAA 
GAGCTGTCTG AGATCACGAC GGCCGATGCC GAGCCCCTGG TTCCTCGAGG AGGCTTCGAC 
TCACCCTTCT ACCGGGACAG TCTGCCGGGC TGCCCGCGGA AGACCCTCTC AGCTTCCTCC 
AGCACTCAGG GCACCAGCGC GAACCCTGAG CCTTTAAACT CCTCCCTGGA CAAGTCGGGG 
CCTGACACAC CAAAGCAAGC CTTCACGCCC ATAGACCTGC CCTGCGGAGG CACCGGGGAG 
AGCCCTGCCG GGAACAGGGA GCGCCAGGCC TCTCTGGAGG CCAGCATCCT CACCCCCAGC 
CCCTGTAAAA TTCCACCTCA GAGGGGAGTG GGGTTTGGCA GTGGGCAGCC TCCCCCATAC 
GATCATCTTT TTGAGGTGGC ATTGCCAAAG ACTGCCCATC ATTTTGTCAG CAAGAAGACG 
GAGGAACTGC TCAAGAAGGC AAAGGGAAGT GCAGAGGAGG ACTTCACGCC CTCCGCCTCC 
CCTATGGAAG TGCTGGACAG GCTGATACAG CAGGGAGCGG ACGTGCACAG CAAGGAGCTC 
AACAGGTTGT CTTTACCCAG CAAGTCTGTC GACTGGACCC ACTTCGGAGG CTCTCCTCCC 
TCAGATGAGA TCTGCACCCT CCGTAACCAG TTGCTTTTAC TGCACAACCA GTTACTCTAC 
GAGCGTTTCA AGCGGCAGCA GCACGCGCTG CGCAACAGGC GGCTCCTGCG CAAGGTGATC 
CGGGCCTCGG CTCTGGAGGA GCACAACGCC GCCATGAAAG ACCAGTTGAA GTTGCAAGAG 
AAAGACATCC AGATATGGAA GATTAGTCTG CAGAAAGAAC AAGCCAGGTA CAATCAGCTT 
CAGGAGCAGC GAGACACAGT GGTCACCCAG CTTCATAGCC AGATCAGACA GCTTCAGCAC 
GACCGGGAGG AGTTCTACAA CCAGAGCCAG GAGTTACAGA CGAAGCTGGA GGACTGCAGG 
AACATGATCG CAGAGCTGCG CGTGGAGCTG AAGAAGGCCA ACAGCAAGGT GTGCCACACC 
GAGCTGCTGC TCAGCCAGGT GTCCCAGAAG CTCTCAAATA GTGAGTCGGT CCAACAGCAG 
ATGGAGTTCT TGAACAGGCA ACTGTTGGTT CTTGGGGAAG TCAACGAGCT GTATTTGGAA 
CAGCTGCAGA ACAAGCATTC AGACACCACC AAGGAAGTAG AAATGATGAA GGCTGCATAT 
CGGAAAGAGC TAGAAAAAAA CAGAAGCCAC GTCCTCCAGC AGAATCAGAG GCTTGATACC 
TGTCAGAAGC GGATTTTGGA ACTGGAATCT CATCTGGCTA AGAAAGACCA CCTTCTTTTA 
GAACAGAAAA AGTATCTAGA GGATGTCAAA CTGCAGGCAA GAGGACAGCT ACAGGCTGCA 
GAGAGCAGGT ATGAGGCCCA AAAGAGGATC ACCCAGGTGT TTGAATTGGA GATCTTAGAT 
TTATACGGCA AGTTGGAGAA GGATGGCCTC CTGAAAAAAC TTGAAGAACA AAAACCAGAA 
GCAGCTGAAG CAGCAGAAGA AAGGCTTGAC TACTGTCAGG ACGGCTGCTC GGATTCGGTG 
ACAGGGCACA ACGAAGAGGC CCCTGGCCGT AACGGTGAGA CCAAGCCCTC CAGGCCTGGC 
GGCGCCCGGG GCGGTGGCGG TGGCGGCGGC AGAGGTGGCG GCAGCAGCAG CGAGCTCTCC 
ACCCCGGAGA AGCCCCCCAA CCCCAGGGCG GGCCCGTTCA GCAGTCGGTG GGAGACGGTG 
GTGGGTGAGC CCACTTCCGG CACCCCCACG ACGGTGGGCT CGCTTCCCAG CTCAGAGAGC 
TTCCTGGGCA TGAAGGCACG AGAGCTGTTT CGTAACAAGA GCGAGAGCCA ATGCGACGAG 
GATGGCCTGA CTGTTGGCAG CCTTTCTGAG ACCCTAAAGA CAGAGCTGGG CAAAGACTCG 
GGTGTGGAAG CCAGGGCCCC CCTGACCCTT GATGGCCCAC TGCTGCCTGC CCCAAAGCCG 
GACAGCGTGG GCCAGCTCCG CATCATGGAC TACAACGAGG CCCAGCTTGA GCACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_010818993.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817295.1 Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X7, mRNA.

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CloneID	OBa07130
Clone ID Related Accession (Same CDS sequence)	XM_010810528.3 , XM_010810528.2 , XM_010818993.2 , XM_024999613.1
Accession Version	XM_010818993.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hamartin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818993.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGGT 
GGGAAAGGAA CTCCTCTGGG AACCCCTGCA ACCTCTCCTC CTCCAGCCCC ACCCTGCCAT 
TCGGACGACT GCGTGCACCT TTCACTCCCA CAGGCCACGG CCCCCAAGAA GGAAGAGAGG 
ACAGACTCCG CAAGGCCCTG TCTACACAGA CAACCCCATG TTCCGAATGA CAGAGGATTA 
GAAGAGCTGC ACGGAGGCAA GGGATCTGTC ACTCTCAGTG ATCTTCCAGG GTTTTTAGGA 
GACCTGGCTT CTGAAGAAGA CAGTATTGAG AAGGATAAAG AAGAAGCGGC CATATCTAAA 
GAGCTGTCTG AGATCACGAC GGCCGATGCC GAGCCCCTGG TTCCTCGAGG AGGCTTCGAC 
TCACCCTTCT ACCGGGACAG TCTGCCGGGC TGCCCGCGGA AGACCCTCTC AGCTTCCTCC 
AGCACTCAGG GCACCAGCGC GAACCCTGAG CCTTTAAACT CCTCCCTGGA CAAGTCGGGG 
CCTGACACAC CAAAGCAAGC CTTCACGCCC ATAGACCTGC CCTGCGGAGG CACCGGGGAG 
AGCCCTGCCG GGAACAGGGA GCGCCAGGCC TCTCTGGAGG CCAGCATCCT CACCCCCAGC 
CCCTGTAAAA TTCCACCTCA GAGGGGAGTG GGGTTTGGCA GTGGGCAGCC TCCCCCATAC 
GATCATCTTT TTGAGGTGGC ATTGCCAAAG ACTGCCCATC ATTTTGTCAG CAAGAAGACG 
GAGGAACTGC TCAAGAAGGC AAAGGGAAGT GCAGAGGAGG ACTTCACGCC CTCCGCCTCC 
CCTATGGAAG TGCTGGACAG GCTGATACAG CAGGGAGCGG ACGTGCACAG CAAGGAGCTC 
AACAGGTTGT CTTTACCCAG CAAGTCTGTC GACTGGACCC ACTTCGGAGG CTCTCCTCCC 
TCAGATGAGA TCTGCACCCT CCGTAACCAG TTGCTTTTAC TGCACAACCA GTTACTCTAC 
GAGCGTTTCA AGCGGCAGCA GCACGCGCTG CGCAACAGGC GGCTCCTGCG CAAGGTGATC 
CGGGCCTCGG CTCTGGAGGA GCACAACGCC GCCATGAAAG ACCAGTTGAA GTTGCAAGAG 
AAAGACATCC AGATATGGAA GATTAGTCTG CAGAAAGAAC AAGCCAGGTA CAATCAGCTT 
CAGGAGCAGC GAGACACAGT GGTCACCCAG CTTCATAGCC AGATCAGACA GCTTCAGCAC 
GACCGGGAGG AGTTCTACAA CCAGAGCCAG GAGTTACAGA CGAAGCTGGA GGACTGCAGG 
AACATGATCG CAGAGCTGCG CGTGGAGCTG AAGAAGGCCA ACAGCAAGGT GTGCCACACC 
GAGCTGCTGC TCAGCCAGGT GTCCCAGAAG CTCTCAAATA GTGAGTCGGT CCAACAGCAG 
ATGGAGTTCT TGAACAGGCA ACTGTTGGTT CTTGGGGAAG TCAACGAGCT GTATTTGGAA 
CAGCTGCAGA ACAAGCATTC AGACACCACC AAGGAAGTAG AAATGATGAA GGCTGCATAT 
CGGAAAGAGC TAGAAAAAAA CAGAAGCCAC GTCCTCCAGC AGAATCAGAG GCTTGATACC 
TGTCAGAAGC GGATTTTGGA ACTGGAATCT CATCTGGCTA AGAAAGACCA CCTTCTTTTA 
GAACAGAAAA AGTATCTAGA GGATGTCAAA CTGCAGGCAA GAGGACAGCT ACAGGCTGCA 
GAGAGCAGGT ATGAGGCCCA AAAGAGGATC ACCCAGGTGT TTGAATTGGA GATCTTAGAT 
TTATACGGCA AGTTGGAGAA GGATGGCCTC CTGAAAAAAC TTGAAGAACA AAAACCAGAA 
GCAGCTGAAG CAGCAGAAGA AAGGCTTGAC TACTGTCAGG ACGGCTGCTC GGATTCGGTG 
ACAGGGCACA ACGAAGAGGC CCCTGGCCGT AACGGTGAGA CCAAGCCCTC CAGGCCTGGC 
GGCGCCCGGG GCGGTGGCGG TGGCGGCGGC AGAGGTGGCG GCAGCAGCAG CGAGCTCTCC 
ACCCCGGAGA AGCCCCCCAA CCCCAGGGCG GGCCCGTTCA GCAGTCGGTG GGAGACGGTG 
GTGGGTGAGC CCACTTCCGG CACCCCCACG ACGGTGGGCT CGCTTCCCAG CTCAGAGAGC 
TTCCTGGGCA TGAAGGCACG AGAGCTGTTT CGTAACAAGA GCGAGAGCCA ATGCGACGAG 
GATGGCCTGA CTGTTGGCAG CCTTTCTGAG ACCCTAAAGA CAGAGCTGGG CAAAGACTCG 
GGTGTGGAAG CCAGGGCCCC CCTGACCCTT GATGGCCCAC TGCTGCCTGC CCCAAAGCCG 
GACAGCGTGG GCCAGCTCCG CATCATGGAC TACAACGAGG CCCAGCTTGA GCACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817295.1
CDS	241..3720
Translation	MAQQANIGELLSMLDSPLLSVRDDVTTVFKENLNSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSALLLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGGSPPSDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_010818993.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818993.2

ORF Insert Sequence:

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGGT 
GGGAAAGGAA CTCCTCTGGG AACCCCTGCA ACCTCTCCTC CTCCAGCCCC ACCCTGCCAT 
TCGGACGACT GCGTGCACCT TTCACTCCCA CAGGCCACGG CCCCCAAGAA GGAAGAGAGG 
ACAGACTCCG CAAGGCCCTG TCTACACAGA CAACCCCATG TTCCGAATGA CAGAGGATTA 
GAAGAGCTGC ACGGAGGCAA GGGATCTGTC ACTCTCAGTG ATCTTCCAGG GTTTTTAGGA 
GACCTGGCTT CTGAAGAAGA CAGTATTGAG AAGGATAAAG AAGAAGCGGC CATATCTAAA 
GAGCTGTCTG AGATCACGAC GGCCGATGCC GAGCCCCTGG TTCCTCGAGG AGGCTTCGAC 
TCACCCTTCT ACCGGGACAG TCTGCCGGGC TGCCCGCGGA AGACCCTCTC AGCTTCCTCC 
AGCACTCAGG GCACCAGCGC GAACCCTGAG CCTTTAAACT CCTCCCTGGA CAAGTCGGGG 
CCTGACACAC CAAAGCAAGC CTTCACGCCC ATAGACCTGC CCTGCGGAGG CACCGGGGAG 
AGCCCTGCCG GGAACAGGGA GCGCCAGGCC TCTCTGGAGG CCAGCATCCT CACCCCCAGC 
CCCTGTAAAA TTCCACCTCA GAGGGGAGTG GGGTTTGGCA GTGGGCAGCC TCCCCCATAC 
GATCATCTTT TTGAGGTGGC ATTGCCAAAG ACTGCCCATC ATTTTGTCAG CAAGAAGACG 
GAGGAACTGC TCAAGAAGGC AAAGGGAAGT GCAGAGGAGG ACTTCACGCC CTCCGCCTCC 
CCTATGGAAG TGCTGGACAG GCTGATACAG CAGGGAGCGG ACGTGCACAG CAAGGAGCTC 
AACAGGTTGT CTTTACCCAG CAAGTCTGTC GACTGGACCC ACTTCGGAGG CTCTCCTCCC 
TCAGATGAGA TCTGCACCCT CCGTAACCAG TTGCTTTTAC TGCACAACCA GTTACTCTAC 
GAGCGTTTCA AGCGGCAGCA GCACGCGCTG CGCAACAGGC GGCTCCTGCG CAAGGTGATC 
CGGGCCTCGG CTCTGGAGGA GCACAACGCC GCCATGAAAG ACCAGTTGAA GTTGCAAGAG 
AAAGACATCC AGATATGGAA GATTAGTCTG CAGAAAGAAC AAGCCAGGTA CAATCAGCTT 
CAGGAGCAGC GAGACACAGT GGTCACCCAG CTTCATAGCC AGATCAGACA GCTTCAGCAC 
GACCGGGAGG AGTTCTACAA CCAGAGCCAG GAGTTACAGA CGAAGCTGGA GGACTGCAGG 
AACATGATCG CAGAGCTGCG CGTGGAGCTG AAGAAGGCCA ACAGCAAGGT GTGCCACACC 
GAGCTGCTGC TCAGCCAGGT GTCCCAGAAG CTCTCAAATA GTGAGTCGGT CCAACAGCAG 
ATGGAGTTCT TGAACAGGCA ACTGTTGGTT CTTGGGGAAG TCAACGAGCT GTATTTGGAA 
CAGCTGCAGA ACAAGCATTC AGACACCACC AAGGAAGTAG AAATGATGAA GGCTGCATAT 
CGGAAAGAGC TAGAAAAAAA CAGAAGCCAC GTCCTCCAGC AGAATCAGAG GCTTGATACC 
TGTCAGAAGC GGATTTTGGA ACTGGAATCT CATCTGGCTA AGAAAGACCA CCTTCTTTTA 
GAACAGAAAA AGTATCTAGA GGATGTCAAA CTGCAGGCAA GAGGACAGCT ACAGGCTGCA 
GAGAGCAGGT ATGAGGCCCA AAAGAGGATC ACCCAGGTGT TTGAATTGGA GATCTTAGAT 
TTATACGGCA AGTTGGAGAA GGATGGCCTC CTGAAAAAAC TTGAAGAACA AAAACCAGAA 
GCAGCTGAAG CAGCAGAAGA AAGGCTTGAC TACTGTCAGG ACGGCTGCTC GGATTCGGTG 
ACAGGGCACA ACGAAGAGGC CCCTGGCCGT AACGGTGAGA CCAAGCCCTC CAGGCCTGGC 
GGCGCCCGGG GCGGTGGCGG TGGCGGCGGC AGAGGTGGCG GCAGCAGCAG CGAGCTCTCC 
ACCCCGGAGA AGCCCCCCAA CCCCAGGGCG GGCCCGTTCA GCAGTCGGTG GGAGACGGTG 
GTGGGTGAGC CCACTTCCGG CACCCCCACG ACGGTGGGCT CGCTTCCCAG CTCAGAGAGC 
TTCCTGGGCA TGAAGGCACG AGAGCTGTTT CGTAACAAGA GCGAGAGCCA ATGCGACGAG 
GATGGCCTGA CTGTTGGCAG CCTTTCTGAG ACCCTAAAGA CAGAGCTGGG CAAAGACTCG 
GGTGTGGAAG CCAGGGCCCC CCTGACCCTT GATGGCCCAC TGCTGCCTGC CCCAAAGCCG 
GACAGCGTGG GCCAGCTCCG CATCATGGAC TACAACGAGG CCCAGCTTGA GCACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_024999613.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855381.1 Bos taurus TSC complex subunit 1 (TSC1), transcript variant X5, mRNA.

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CloneID	OBa07130
Clone ID Related Accession (Same CDS sequence)	XM_010810528.3 , XM_010810528.2 , XM_010818993.2 , XM_024999613.1
Accession Version	XM_024999613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	hamartin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGGT 
GGGAAAGGAA CTCCTCTGGG AACCCCTGCA ACCTCTCCTC CTCCAGCCCC ACCCTGCCAT 
TCGGACGACT GCGTGCACCT TTCACTCCCA CAGGCCACGG CCCCCAAGAA GGAAGAGAGG 
ACAGACTCCG CAAGGCCCTG TCTACACAGA CAACCCCATG TTCCGAATGA CAGAGGATTA 
GAAGAGCTGC ACGGAGGCAA GGGATCTGTC ACTCTCAGTG ATCTTCCAGG GTTTTTAGGA 
GACCTGGCTT CTGAAGAAGA CAGTATTGAG AAGGATAAAG AAGAAGCGGC CATATCTAAA 
GAGCTGTCTG AGATCACGAC GGCCGATGCC GAGCCCCTGG TTCCTCGAGG AGGCTTCGAC 
TCACCCTTCT ACCGGGACAG TCTGCCGGGC TGCCCGCGGA AGACCCTCTC AGCTTCCTCC 
AGCACTCAGG GCACCAGCGC GAACCCTGAG CCTTTAAACT CCTCCCTGGA CAAGTCGGGG 
CCTGACACAC CAAAGCAAGC CTTCACGCCC ATAGACCTGC CCTGCGGAGG CACCGGGGAG 
AGCCCTGCCG GGAACAGGGA GCGCCAGGCC TCTCTGGAGG CCAGCATCCT CACCCCCAGC 
CCCTGTAAAA TTCCACCTCA GAGGGGAGTG GGGTTTGGCA GTGGGCAGCC TCCCCCATAC 
GATCATCTTT TTGAGGTGGC ATTGCCAAAG ACTGCCCATC ATTTTGTCAG CAAGAAGACG 
GAGGAACTGC TCAAGAAGGC AAAGGGAAGT GCAGAGGAGG ACTTCACGCC CTCCGCCTCC 
CCTATGGAAG TGCTGGACAG GCTGATACAG CAGGGAGCGG ACGTGCACAG CAAGGAGCTC 
AACAGGTTGT CTTTACCCAG CAAGTCTGTC GACTGGACCC ACTTCGGAGG CTCTCCTCCC 
TCAGATGAGA TCTGCACCCT CCGTAACCAG TTGCTTTTAC TGCACAACCA GTTACTCTAC 
GAGCGTTTCA AGCGGCAGCA GCACGCGCTG CGCAACAGGC GGCTCCTGCG CAAGGTGATC 
CGGGCCTCGG CTCTGGAGGA GCACAACGCC GCCATGAAAG ACCAGTTGAA GTTGCAAGAG 
AAAGACATCC AGATATGGAA GATTAGTCTG CAGAAAGAAC AAGCCAGGTA CAATCAGCTT 
CAGGAGCAGC GAGACACAGT GGTCACCCAG CTTCATAGCC AGATCAGACA GCTTCAGCAC 
GACCGGGAGG AGTTCTACAA CCAGAGCCAG GAGTTACAGA CGAAGCTGGA GGACTGCAGG 
AACATGATCG CAGAGCTGCG CGTGGAGCTG AAGAAGGCCA ACAGCAAGGT GTGCCACACC 
GAGCTGCTGC TCAGCCAGGT GTCCCAGAAG CTCTCAAATA GTGAGTCGGT CCAACAGCAG 
ATGGAGTTCT TGAACAGGCA ACTGTTGGTT CTTGGGGAAG TCAACGAGCT GTATTTGGAA 
CAGCTGCAGA ACAAGCATTC AGACACCACC AAGGAAGTAG AAATGATGAA GGCTGCATAT 
CGGAAAGAGC TAGAAAAAAA CAGAAGCCAC GTCCTCCAGC AGAATCAGAG GCTTGATACC 
TGTCAGAAGC GGATTTTGGA ACTGGAATCT CATCTGGCTA AGAAAGACCA CCTTCTTTTA 
GAACAGAAAA AGTATCTAGA GGATGTCAAA CTGCAGGCAA GAGGACAGCT ACAGGCTGCA 
GAGAGCAGGT ATGAGGCCCA AAAGAGGATC ACCCAGGTGT TTGAATTGGA GATCTTAGAT 
TTATACGGCA AGTTGGAGAA GGATGGCCTC CTGAAAAAAC TTGAAGAACA AAAACCAGAA 
GCAGCTGAAG CAGCAGAAGA AAGGCTTGAC TACTGTCAGG ACGGCTGCTC GGATTCGGTG 
ACAGGGCACA ACGAAGAGGC CCCTGGCCGT AACGGTGAGA CCAAGCCCTC CAGGCCTGGC 
GGCGCCCGGG GCGGTGGCGG TGGCGGCGGC AGAGGTGGCG GCAGCAGCAG CGAGCTCTCC 
ACCCCGGAGA AGCCCCCCAA CCCCAGGGCG GGCCCGTTCA GCAGTCGGTG GGAGACGGTG 
GTGGGTGAGC CCACTTCCGG CACCCCCACG ACGGTGGGCT CGCTTCCCAG CTCAGAGAGC 
TTCCTGGGCA TGAAGGCACG AGAGCTGTTT CGTAACAAGA GCGAGAGCCA ATGCGACGAG 
GATGGCCTGA CTGTTGGCAG CCTTTCTGAG ACCCTAAAGA CAGAGCTGGG CAAAGACTCG 
GGTGTGGAAG CCAGGGCCCC CCTGACCCTT GATGGCCCAC TGCTGCCTGC CCCAAAGCCG 
GACAGCGTGG GCCAGCTCCG CATCATGGAC TACAACGAGG CCCAGCTTGA GCACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855381.1
CDS	348..3827
Translation	MAQQANIGELLSMLDSPLLSVRDDVTTVFKENLNSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSALLLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGGSPPSDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_024999613.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TSC complex subunit 1 (TSC1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999613.1

ORF Insert Sequence:

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGGT 
GGGAAAGGAA CTCCTCTGGG AACCCCTGCA ACCTCTCCTC CTCCAGCCCC ACCCTGCCAT 
TCGGACGACT GCGTGCACCT TTCACTCCCA CAGGCCACGG CCCCCAAGAA GGAAGAGAGG 
ACAGACTCCG CAAGGCCCTG TCTACACAGA CAACCCCATG TTCCGAATGA CAGAGGATTA 
GAAGAGCTGC ACGGAGGCAA GGGATCTGTC ACTCTCAGTG ATCTTCCAGG GTTTTTAGGA 
GACCTGGCTT CTGAAGAAGA CAGTATTGAG AAGGATAAAG AAGAAGCGGC CATATCTAAA 
GAGCTGTCTG AGATCACGAC GGCCGATGCC GAGCCCCTGG TTCCTCGAGG AGGCTTCGAC 
TCACCCTTCT ACCGGGACAG TCTGCCGGGC TGCCCGCGGA AGACCCTCTC AGCTTCCTCC 
AGCACTCAGG GCACCAGCGC GAACCCTGAG CCTTTAAACT CCTCCCTGGA CAAGTCGGGG 
CCTGACACAC CAAAGCAAGC CTTCACGCCC ATAGACCTGC CCTGCGGAGG CACCGGGGAG 
AGCCCTGCCG GGAACAGGGA GCGCCAGGCC TCTCTGGAGG CCAGCATCCT CACCCCCAGC 
CCCTGTAAAA TTCCACCTCA GAGGGGAGTG GGGTTTGGCA GTGGGCAGCC TCCCCCATAC 
GATCATCTTT TTGAGGTGGC ATTGCCAAAG ACTGCCCATC ATTTTGTCAG CAAGAAGACG 
GAGGAACTGC TCAAGAAGGC AAAGGGAAGT GCAGAGGAGG ACTTCACGCC CTCCGCCTCC 
CCTATGGAAG TGCTGGACAG GCTGATACAG CAGGGAGCGG ACGTGCACAG CAAGGAGCTC 
AACAGGTTGT CTTTACCCAG CAAGTCTGTC GACTGGACCC ACTTCGGAGG CTCTCCTCCC 
TCAGATGAGA TCTGCACCCT CCGTAACCAG TTGCTTTTAC TGCACAACCA GTTACTCTAC 
GAGCGTTTCA AGCGGCAGCA GCACGCGCTG CGCAACAGGC GGCTCCTGCG CAAGGTGATC 
CGGGCCTCGG CTCTGGAGGA GCACAACGCC GCCATGAAAG ACCAGTTGAA GTTGCAAGAG 
AAAGACATCC AGATATGGAA GATTAGTCTG CAGAAAGAAC AAGCCAGGTA CAATCAGCTT 
CAGGAGCAGC GAGACACAGT GGTCACCCAG CTTCATAGCC AGATCAGACA GCTTCAGCAC 
GACCGGGAGG AGTTCTACAA CCAGAGCCAG GAGTTACAGA CGAAGCTGGA GGACTGCAGG 
AACATGATCG CAGAGCTGCG CGTGGAGCTG AAGAAGGCCA ACAGCAAGGT GTGCCACACC 
GAGCTGCTGC TCAGCCAGGT GTCCCAGAAG CTCTCAAATA GTGAGTCGGT CCAACAGCAG 
ATGGAGTTCT TGAACAGGCA ACTGTTGGTT CTTGGGGAAG TCAACGAGCT GTATTTGGAA 
CAGCTGCAGA ACAAGCATTC AGACACCACC AAGGAAGTAG AAATGATGAA GGCTGCATAT 
CGGAAAGAGC TAGAAAAAAA CAGAAGCCAC GTCCTCCAGC AGAATCAGAG GCTTGATACC 
TGTCAGAAGC GGATTTTGGA ACTGGAATCT CATCTGGCTA AGAAAGACCA CCTTCTTTTA 
GAACAGAAAA AGTATCTAGA GGATGTCAAA CTGCAGGCAA GAGGACAGCT ACAGGCTGCA 
GAGAGCAGGT ATGAGGCCCA AAAGAGGATC ACCCAGGTGT TTGAATTGGA GATCTTAGAT 
TTATACGGCA AGTTGGAGAA GGATGGCCTC CTGAAAAAAC TTGAAGAACA AAAACCAGAA 
GCAGCTGAAG CAGCAGAAGA AAGGCTTGAC TACTGTCAGG ACGGCTGCTC GGATTCGGTG 
ACAGGGCACA ACGAAGAGGC CCCTGGCCGT AACGGTGAGA CCAAGCCCTC CAGGCCTGGC 
GGCGCCCGGG GCGGTGGCGG TGGCGGCGGC AGAGGTGGCG GCAGCAGCAG CGAGCTCTCC 
ACCCCGGAGA AGCCCCCCAA CCCCAGGGCG GGCCCGTTCA GCAGTCGGTG GGAGACGGTG 
GTGGGTGAGC CCACTTCCGG CACCCCCACG ACGGTGGGCT CGCTTCCCAG CTCAGAGAGC 
TTCCTGGGCA TGAAGGCACG AGAGCTGTTT CGTAACAAGA GCGAGAGCCA ATGCGACGAG 
GATGGCCTGA CTGTTGGCAG CCTTTCTGAG ACCCTAAAGA CAGAGCTGGG CAAAGACTCG 
GGTGTGGAAG CCAGGGCCCC CCTGACCCTT GATGGCCCAC TGCTGCCTGC CCCAAAGCCG 
GACAGCGTGG GCCAGCTCCG CATCATGGAC TACAACGAGG CCCAGCTTGA GCACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_010810529.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808831.1 Bos taurus TSC complex subunit 1 (TSC1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa52450
Clone ID Related Accession (Same CDS sequence)	XM_010810529.3 , XM_010810529.2 , XM_010818996.2
Accession Version	XM_010810529.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	hamartin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810529.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGATGAGATC 
TGCACCCTCC GTAACCAGTT GCTTTTACTG CACAACCAGT TACTCTACGA GCGTTTCAAG 
CGGCAGCAGC ACGCGCTGCG CAACAGGCGG CTCCTGCGCA AGGTGATCCG GGCCTCGGCT 
CTGGAGGAGC ACAACGCCGC CATGAAAGAC CAGTTGAAGT TGCAAGAGAA AGACATCCAG 
ATATGGAAGA TTAGTCTGCA GAAAGAACAA GCCAGGTACA ATCAGCTTCA GGAGCAGCGA 
GACACAGTGG TCACCCAGCT TCATAGCCAG ATCAGACAGC TTCAGCACGA CCGGGAGGAG 
TTCTACAACC AGAGCCAGGA GTTACAGACG AAGCTGGAGG ACTGCAGGAA CATGATCGCA 
GAGCTGCGCG TGGAGCTGAA GAAGGCCAAC AGCAAGGTGT GCCACACCGA GCTGCTGCTC 
AGCCAGGTGT CCCAGAAGCT CTCAAATAGT GAGTCGGTCC AACAGCAGAT GGAGTTCTTG 
AACAGGCAAC TGTTGGTTCT TGGGGAAGTC AACGAGCTGT ATTTGGAACA GCTGCAGAAC 
AAGCATTCAG ACACCACCAA GGAAGTAGAA ATGATGAAGG CTGCATATCG GAAAGAGCTA 
GAAAAAAACA GAAGCCACGT CCTCCAGCAG AATCAGAGGC TTGATACCTG TCAGAAGCGG 
ATTTTGGAAC TGGAATCTCA TCTGGCTAAG AAAGACCACC TTCTTTTAGA ACAGAAAAAG 
TATCTAGAGG ATGTCAAACT GCAGGCAAGA GGACAGCTAC AGGCTGCAGA GAGCAGGTAT 
GAGGCCCAAA AGAGGATCAC CCAGGTGTTT GAATTGGAGA TCTTAGATTT ATACGGCAAG 
TTGGAGAAGG ATGGCCTCCT GAAAAAACTT GAAGAACAAA AACCAGAAGC AGCTGAAGCA 
GCAGAAGAAA GGCTTGACTA CTGTCAGGAC GGCTGCTCGG ATTCGGTGAC AGGGCACAAC 
GAAGAGGCCC CTGGCCGTAA CGGTGAGACC AAGCCCTCCA GGCCTGGCGG CGCCCGGGGC 
GGTGGCGGTG GCGGCGGCAG AGGTGGCGGC AGCAGCAGCG AGCTCTCCAC CCCGGAGAAG 
CCCCCCAACC CCAGGGCGGG CCCGTTCAGC AGTCGGTGGG AGACGGTGGT GGGTGAGCCC 
ACTTCCGGCA CCCCCACGAC GGTGGGCTCG CTTCCCAGCT CAGAGAGCTT CCTGGGCATG 
AAGGCACGAG AGCTGTTTCG TAACAAGAGC GAGAGCCAAT GCGACGAGGA TGGCCTGACT 
GTTGGCAGCC TTTCTGAGAC CCTAAAGACA GAGCTGGGCA AAGACTCGGG TGTGGAAGCC 
AGGGCCCCCC TGACCCTTGA TGGCCCACTG CTGCCTGCCC CAAAGCCGGA CAGCGTGGGC 
CAGCTCCGCA TCATGGACTA CAACGAGGCC CAGCTTGAGC ACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808831.1
CDS	382..3849
Translation	MAQQANIGELLSMLDSPLLSVRDDVTTVFKENLNSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSALLLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_010810529.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TSC complex subunit 1 (TSC1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810529.3

ORF Insert Sequence:

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGATGAGATC 
TGCACCCTCC GTAACCAGTT GCTTTTACTG CACAACCAGT TACTCTACGA GCGTTTCAAG 
CGGCAGCAGC ACGCGCTGCG CAACAGGCGG CTCCTGCGCA AGGTGATCCG GGCCTCGGCT 
CTGGAGGAGC ACAACGCCGC CATGAAAGAC CAGTTGAAGT TGCAAGAGAA AGACATCCAG 
ATATGGAAGA TTAGTCTGCA GAAAGAACAA GCCAGGTACA ATCAGCTTCA GGAGCAGCGA 
GACACAGTGG TCACCCAGCT TCATAGCCAG ATCAGACAGC TTCAGCACGA CCGGGAGGAG 
TTCTACAACC AGAGCCAGGA GTTACAGACG AAGCTGGAGG ACTGCAGGAA CATGATCGCA 
GAGCTGCGCG TGGAGCTGAA GAAGGCCAAC AGCAAGGTGT GCCACACCGA GCTGCTGCTC 
AGCCAGGTGT CCCAGAAGCT CTCAAATAGT GAGTCGGTCC AACAGCAGAT GGAGTTCTTG 
AACAGGCAAC TGTTGGTTCT TGGGGAAGTC AACGAGCTGT ATTTGGAACA GCTGCAGAAC 
AAGCATTCAG ACACCACCAA GGAAGTAGAA ATGATGAAGG CTGCATATCG GAAAGAGCTA 
GAAAAAAACA GAAGCCACGT CCTCCAGCAG AATCAGAGGC TTGATACCTG TCAGAAGCGG 
ATTTTGGAAC TGGAATCTCA TCTGGCTAAG AAAGACCACC TTCTTTTAGA ACAGAAAAAG 
TATCTAGAGG ATGTCAAACT GCAGGCAAGA GGACAGCTAC AGGCTGCAGA GAGCAGGTAT 
GAGGCCCAAA AGAGGATCAC CCAGGTGTTT GAATTGGAGA TCTTAGATTT ATACGGCAAG 
TTGGAGAAGG ATGGCCTCCT GAAAAAACTT GAAGAACAAA AACCAGAAGC AGCTGAAGCA 
GCAGAAGAAA GGCTTGACTA CTGTCAGGAC GGCTGCTCGG ATTCGGTGAC AGGGCACAAC 
GAAGAGGCCC CTGGCCGTAA CGGTGAGACC AAGCCCTCCA GGCCTGGCGG CGCCCGGGGC 
GGTGGCGGTG GCGGCGGCAG AGGTGGCGGC AGCAGCAGCG AGCTCTCCAC CCCGGAGAAG 
CCCCCCAACC CCAGGGCGGG CCCGTTCAGC AGTCGGTGGG AGACGGTGGT GGGTGAGCCC 
ACTTCCGGCA CCCCCACGAC GGTGGGCTCG CTTCCCAGCT CAGAGAGCTT CCTGGGCATG 
AAGGCACGAG AGCTGTTTCG TAACAAGAGC GAGAGCCAAT GCGACGAGGA TGGCCTGACT 
GTTGGCAGCC TTTCTGAGAC CCTAAAGACA GAGCTGGGCA AAGACTCGGG TGTGGAAGCC 
AGGGCCCCCC TGACCCTTGA TGGCCCACTG CTGCCTGCCC CAAAGCCGGA CAGCGTGGGC 
CAGCTCCGCA TCATGGACTA CAACGAGGCC CAGCTTGAGC ACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_010810529.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808831.1 Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X8, mRNA.

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CloneID	OBa52450
Clone ID Related Accession (Same CDS sequence)	XM_010810529.3 , XM_010810529.2 , XM_010818996.2
Accession Version	XM_010810529.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hamartin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810529.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGATGAGATC 
TGCACCCTCC GTAACCAGTT GCTTTTACTG CACAACCAGT TACTCTACGA GCGTTTCAAG 
CGGCAGCAGC ACGCGCTGCG CAACAGGCGG CTCCTGCGCA AGGTGATCCG GGCCTCGGCT 
CTGGAGGAGC ACAACGCCGC CATGAAAGAC CAGTTGAAGT TGCAAGAGAA AGACATCCAG 
ATATGGAAGA TTAGTCTGCA GAAAGAACAA GCCAGGTACA ATCAGCTTCA GGAGCAGCGA 
GACACAGTGG TCACCCAGCT TCATAGCCAG ATCAGACAGC TTCAGCACGA CCGGGAGGAG 
TTCTACAACC AGAGCCAGGA GTTACAGACG AAGCTGGAGG ACTGCAGGAA CATGATCGCA 
GAGCTGCGCG TGGAGCTGAA GAAGGCCAAC AGCAAGGTGT GCCACACCGA GCTGCTGCTC 
AGCCAGGTGT CCCAGAAGCT CTCAAATAGT GAGTCGGTCC AACAGCAGAT GGAGTTCTTG 
AACAGGCAAC TGTTGGTTCT TGGGGAAGTC AACGAGCTGT ATTTGGAACA GCTGCAGAAC 
AAGCATTCAG ACACCACCAA GGAAGTAGAA ATGATGAAGG CTGCATATCG GAAAGAGCTA 
GAAAAAAACA GAAGCCACGT CCTCCAGCAG AATCAGAGGC TTGATACCTG TCAGAAGCGG 
ATTTTGGAAC TGGAATCTCA TCTGGCTAAG AAAGACCACC TTCTTTTAGA ACAGAAAAAG 
TATCTAGAGG ATGTCAAACT GCAGGCAAGA GGACAGCTAC AGGCTGCAGA GAGCAGGTAT 
GAGGCCCAAA AGAGGATCAC CCAGGTGTTT GAATTGGAGA TCTTAGATTT ATACGGCAAG 
TTGGAGAAGG ATGGCCTCCT GAAAAAACTT GAAGAACAAA AACCAGAAGC AGCTGAAGCA 
GCAGAAGAAA GGCTTGACTA CTGTCAGGAC GGCTGCTCGG ATTCGGTGAC AGGGCACAAC 
GAAGAGGCCC CTGGCCGTAA CGGTGAGACC AAGCCCTCCA GGCCTGGCGG CGCCCGGGGC 
GGTGGCGGTG GCGGCGGCAG AGGTGGCGGC AGCAGCAGCG AGCTCTCCAC CCCGGAGAAG 
CCCCCCAACC CCAGGGCGGG CCCGTTCAGC AGTCGGTGGG AGACGGTGGT GGGTGAGCCC 
ACTTCCGGCA CCCCCACGAC GGTGGGCTCG CTTCCCAGCT CAGAGAGCTT CCTGGGCATG 
AAGGCACGAG AGCTGTTTCG TAACAAGAGC GAGAGCCAAT GCGACGAGGA TGGCCTGACT 
GTTGGCAGCC TTTCTGAGAC CCTAAAGACA GAGCTGGGCA AAGACTCGGG TGTGGAAGCC 
AGGGCCCCCC TGACCCTTGA TGGCCCACTG CTGCCTGCCC CAAAGCCGGA CAGCGTGGGC 
CAGCTCCGCA TCATGGACTA CAACGAGGCC CAGCTTGAGC ACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808831.1
CDS	241..3708
Translation	MAQQANIGELLSMLDSPLLSVRDDVTTVFKENLNSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSALLLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_010810529.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810529.2

ORF Insert Sequence:

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGATGAGATC 
TGCACCCTCC GTAACCAGTT GCTTTTACTG CACAACCAGT TACTCTACGA GCGTTTCAAG 
CGGCAGCAGC ACGCGCTGCG CAACAGGCGG CTCCTGCGCA AGGTGATCCG GGCCTCGGCT 
CTGGAGGAGC ACAACGCCGC CATGAAAGAC CAGTTGAAGT TGCAAGAGAA AGACATCCAG 
ATATGGAAGA TTAGTCTGCA GAAAGAACAA GCCAGGTACA ATCAGCTTCA GGAGCAGCGA 
GACACAGTGG TCACCCAGCT TCATAGCCAG ATCAGACAGC TTCAGCACGA CCGGGAGGAG 
TTCTACAACC AGAGCCAGGA GTTACAGACG AAGCTGGAGG ACTGCAGGAA CATGATCGCA 
GAGCTGCGCG TGGAGCTGAA GAAGGCCAAC AGCAAGGTGT GCCACACCGA GCTGCTGCTC 
AGCCAGGTGT CCCAGAAGCT CTCAAATAGT GAGTCGGTCC AACAGCAGAT GGAGTTCTTG 
AACAGGCAAC TGTTGGTTCT TGGGGAAGTC AACGAGCTGT ATTTGGAACA GCTGCAGAAC 
AAGCATTCAG ACACCACCAA GGAAGTAGAA ATGATGAAGG CTGCATATCG GAAAGAGCTA 
GAAAAAAACA GAAGCCACGT CCTCCAGCAG AATCAGAGGC TTGATACCTG TCAGAAGCGG 
ATTTTGGAAC TGGAATCTCA TCTGGCTAAG AAAGACCACC TTCTTTTAGA ACAGAAAAAG 
TATCTAGAGG ATGTCAAACT GCAGGCAAGA GGACAGCTAC AGGCTGCAGA GAGCAGGTAT 
GAGGCCCAAA AGAGGATCAC CCAGGTGTTT GAATTGGAGA TCTTAGATTT ATACGGCAAG 
TTGGAGAAGG ATGGCCTCCT GAAAAAACTT GAAGAACAAA AACCAGAAGC AGCTGAAGCA 
GCAGAAGAAA GGCTTGACTA CTGTCAGGAC GGCTGCTCGG ATTCGGTGAC AGGGCACAAC 
GAAGAGGCCC CTGGCCGTAA CGGTGAGACC AAGCCCTCCA GGCCTGGCGG CGCCCGGGGC 
GGTGGCGGTG GCGGCGGCAG AGGTGGCGGC AGCAGCAGCG AGCTCTCCAC CCCGGAGAAG 
CCCCCCAACC CCAGGGCGGG CCCGTTCAGC AGTCGGTGGG AGACGGTGGT GGGTGAGCCC 
ACTTCCGGCA CCCCCACGAC GGTGGGCTCG CTTCCCAGCT CAGAGAGCTT CCTGGGCATG 
AAGGCACGAG AGCTGTTTCG TAACAAGAGC GAGAGCCAAT GCGACGAGGA TGGCCTGACT 
GTTGGCAGCC TTTCTGAGAC CCTAAAGACA GAGCTGGGCA AAGACTCGGG TGTGGAAGCC 
AGGGCCCCCC TGACCCTTGA TGGCCCACTG CTGCCTGCCC CAAAGCCGGA CAGCGTGGGC 
CAGCTCCGCA TCATGGACTA CAACGAGGCC CAGCTTGAGC ACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_010818996.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817298.1 Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa52450
Clone ID Related Accession (Same CDS sequence)	XM_010810529.3 , XM_010810529.2 , XM_010818996.2
Accession Version	XM_010818996.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hamartin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818996.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGATGAGATC 
TGCACCCTCC GTAACCAGTT GCTTTTACTG CACAACCAGT TACTCTACGA GCGTTTCAAG 
CGGCAGCAGC ACGCGCTGCG CAACAGGCGG CTCCTGCGCA AGGTGATCCG GGCCTCGGCT 
CTGGAGGAGC ACAACGCCGC CATGAAAGAC CAGTTGAAGT TGCAAGAGAA AGACATCCAG 
ATATGGAAGA TTAGTCTGCA GAAAGAACAA GCCAGGTACA ATCAGCTTCA GGAGCAGCGA 
GACACAGTGG TCACCCAGCT TCATAGCCAG ATCAGACAGC TTCAGCACGA CCGGGAGGAG 
TTCTACAACC AGAGCCAGGA GTTACAGACG AAGCTGGAGG ACTGCAGGAA CATGATCGCA 
GAGCTGCGCG TGGAGCTGAA GAAGGCCAAC AGCAAGGTGT GCCACACCGA GCTGCTGCTC 
AGCCAGGTGT CCCAGAAGCT CTCAAATAGT GAGTCGGTCC AACAGCAGAT GGAGTTCTTG 
AACAGGCAAC TGTTGGTTCT TGGGGAAGTC AACGAGCTGT ATTTGGAACA GCTGCAGAAC 
AAGCATTCAG ACACCACCAA GGAAGTAGAA ATGATGAAGG CTGCATATCG GAAAGAGCTA 
GAAAAAAACA GAAGCCACGT CCTCCAGCAG AATCAGAGGC TTGATACCTG TCAGAAGCGG 
ATTTTGGAAC TGGAATCTCA TCTGGCTAAG AAAGACCACC TTCTTTTAGA ACAGAAAAAG 
TATCTAGAGG ATGTCAAACT GCAGGCAAGA GGACAGCTAC AGGCTGCAGA GAGCAGGTAT 
GAGGCCCAAA AGAGGATCAC CCAGGTGTTT GAATTGGAGA TCTTAGATTT ATACGGCAAG 
TTGGAGAAGG ATGGCCTCCT GAAAAAACTT GAAGAACAAA AACCAGAAGC AGCTGAAGCA 
GCAGAAGAAA GGCTTGACTA CTGTCAGGAC GGCTGCTCGG ATTCGGTGAC AGGGCACAAC 
GAAGAGGCCC CTGGCCGTAA CGGTGAGACC AAGCCCTCCA GGCCTGGCGG CGCCCGGGGC 
GGTGGCGGTG GCGGCGGCAG AGGTGGCGGC AGCAGCAGCG AGCTCTCCAC CCCGGAGAAG 
CCCCCCAACC CCAGGGCGGG CCCGTTCAGC AGTCGGTGGG AGACGGTGGT GGGTGAGCCC 
ACTTCCGGCA CCCCCACGAC GGTGGGCTCG CTTCCCAGCT CAGAGAGCTT CCTGGGCATG 
AAGGCACGAG AGCTGTTTCG TAACAAGAGC GAGAGCCAAT GCGACGAGGA TGGCCTGACT 
GTTGGCAGCC TTTCTGAGAC CCTAAAGACA GAGCTGGGCA AAGACTCGGG TGTGGAAGCC 
AGGGCCCCCC TGACCCTTGA TGGCCCACTG CTGCCTGCCC CAAAGCCGGA CAGCGTGGGC 
CAGCTCCGCA TCATGGACTA CAACGAGGCC CAGCTTGAGC ACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817298.1
CDS	241..3708
Translation	MAQQANIGELLSMLDSPLLSVRDDVTTVFKENLNSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSALLLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_010818996.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818996.2

ORF Insert Sequence:

ATGGCCCAGC AAGCCAATAT TGGGGAGCTC CTCTCCATGC TGGACTCCCC TCTGCTGAGT 
GTGCGTGATG ATGTAACAAC TGTCTTTAAA GAGAATCTCA ATTCTGACCG TGGTCCTATG 
CTTGTGAACA CCCTGGTGGA TTATTATCTG GAAACCAACT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC CCATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAGGCCG CCTCTCGGTT ATCCGCCCTC TTGCTACTGG GCCACGTCGT GAGGCTGCAG 
CCGTCATGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTCTACT AAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGAGTCT TAGTGTTGAT AACCATGCTC 
CCAATGATTC CGCAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT CTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAGTCTATCT CGTCCACCTC 
CACGCCAGCG TCTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACCTGG AGACTTTTGA AGAAGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAGGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA CCGTTCAGCC GATGTCTCCG CCAGCTCTTA CGTTGACACA 
CAGAATAGCT ATGGGAATGC TACTTCTACC CCCTACTCTA CTTCTCGGCT GACGTTAAAC 
ACGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCGTCGA CACGGCTATT AGCTGAGCCG 
CCACAAGCTA CTCTTTGGAG CCCATCTGCA GTTTGTGGGA TGACCACTCC TCCAACTTCT 
CCTGGAAATG TCCCACCTGA TCTGTCACAT CCTTACAGTA AAGTCTTTGG TACAACTGCA 
GGTGGGAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGACG ACTGCGTGCA CCTTTCACTC CCACAGGCCA CGGCCCCCAA GAAGGAAGAG 
AGGACAGACT CCGCAAGGCC CTGTCTACAC AGACAACCCC ATGTTCCGAA TGACAGAGGA 
TTAGAAGAGC TGCACGGAGG CAAGGGATCT GTCACTCTCA GTGATCTTCC AGGGTTTTTA 
GGAGACCTGG CTTCTGAAGA AGACAGTATT GAGAAGGATA AAGAAGAAGC GGCCATATCT 
AAAGAGCTGT CTGAGATCAC GACGGCCGAT GCCGAGCCCC TGGTTCCTCG AGGAGGCTTC 
GACTCACCCT TCTACCGGGA CAGTCTGCCG GGCTGCCCGC GGAAGACCCT CTCAGCTTCC 
TCCAGCACTC AGGGCACCAG CGCGAACCCT GAGCCTTTAA ACTCCTCCCT GGACAAGTCG 
GGGCCTGACA CACCAAAGCA AGCCTTCACG CCCATAGACC TGCCCTGCGG AGGCACCGGG 
GAGAGCCCTG CCGGGAACAG GGAGCGCCAG GCCTCTCTGG AGGCCAGCAT CCTCACCCCC 
AGCCCCTGTA AAATTCCACC TCAGAGGGGA GTGGGGTTTG GCAGTGGGCA GCCTCCCCCA 
TACGATCATC TTTTTGAGGT GGCATTGCCA AAGACTGCCC ATCATTTTGT CAGCAAGAAG 
ACGGAGGAAC TGCTCAAGAA GGCAAAGGGA AGTGCAGAGG AGGACTTCAC GCCCTCCGCC 
TCCCCTATGG AAGTGCTGGA CAGGCTGATA CAGCAGGGAG CGGACGTGCA CAGCAAGGAG 
CTCAACAGGT TGTCTTTACC CAGCAAGTCT GTCGACTGGA CCCACTTCGG AGATGAGATC 
TGCACCCTCC GTAACCAGTT GCTTTTACTG CACAACCAGT TACTCTACGA GCGTTTCAAG 
CGGCAGCAGC ACGCGCTGCG CAACAGGCGG CTCCTGCGCA AGGTGATCCG GGCCTCGGCT 
CTGGAGGAGC ACAACGCCGC CATGAAAGAC CAGTTGAAGT TGCAAGAGAA AGACATCCAG 
ATATGGAAGA TTAGTCTGCA GAAAGAACAA GCCAGGTACA ATCAGCTTCA GGAGCAGCGA 
GACACAGTGG TCACCCAGCT TCATAGCCAG ATCAGACAGC TTCAGCACGA CCGGGAGGAG 
TTCTACAACC AGAGCCAGGA GTTACAGACG AAGCTGGAGG ACTGCAGGAA CATGATCGCA 
GAGCTGCGCG TGGAGCTGAA GAAGGCCAAC AGCAAGGTGT GCCACACCGA GCTGCTGCTC 
AGCCAGGTGT CCCAGAAGCT CTCAAATAGT GAGTCGGTCC AACAGCAGAT GGAGTTCTTG 
AACAGGCAAC TGTTGGTTCT TGGGGAAGTC AACGAGCTGT ATTTGGAACA GCTGCAGAAC 
AAGCATTCAG ACACCACCAA GGAAGTAGAA ATGATGAAGG CTGCATATCG GAAAGAGCTA 
GAAAAAAACA GAAGCCACGT CCTCCAGCAG AATCAGAGGC TTGATACCTG TCAGAAGCGG 
ATTTTGGAAC TGGAATCTCA TCTGGCTAAG AAAGACCACC TTCTTTTAGA ACAGAAAAAG 
TATCTAGAGG ATGTCAAACT GCAGGCAAGA GGACAGCTAC AGGCTGCAGA GAGCAGGTAT 
GAGGCCCAAA AGAGGATCAC CCAGGTGTTT GAATTGGAGA TCTTAGATTT ATACGGCAAG 
TTGGAGAAGG ATGGCCTCCT GAAAAAACTT GAAGAACAAA AACCAGAAGC AGCTGAAGCA 
GCAGAAGAAA GGCTTGACTA CTGTCAGGAC GGCTGCTCGG ATTCGGTGAC AGGGCACAAC 
GAAGAGGCCC CTGGCCGTAA CGGTGAGACC AAGCCCTCCA GGCCTGGCGG CGCCCGGGGC 
GGTGGCGGTG GCGGCGGCAG AGGTGGCGGC AGCAGCAGCG AGCTCTCCAC CCCGGAGAAG 
CCCCCCAACC CCAGGGCGGG CCCGTTCAGC AGTCGGTGGG AGACGGTGGT GGGTGAGCCC 
ACTTCCGGCA CCCCCACGAC GGTGGGCTCG CTTCCCAGCT CAGAGAGCTT CCTGGGCATG 
AAGGCACGAG AGCTGTTTCG TAACAAGAGC GAGAGCCAAT GCGACGAGGA TGGCCTGACT 
GTTGGCAGCC TTTCTGAGAC CCTAAAGACA GAGCTGGGCA AAGACTCGGG TGTGGAAGCC 
AGGGCCCCCC TGACCCTTGA TGGCCCACTG CTGCCTGCCC CAAAGCCGGA CAGCGTGGGC 
CAGCTCCGCA TCATGGACTA CAACGAGGCC CAGCTTGAGC ACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_010818998.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817300.1 Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X9, mRNA.

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CloneID	OBa52451
Clone ID Related Accession (Same CDS sequence)	XM_010818998.2 , XM_010810530.2 , XM_010810530.3
Accession Version	XM_010818998.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hamartin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818998.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCAC GCCTCTGTGG GCCACTCCAA GTAGATGGGA GTTTATCCAG AGGCCAGGGG 
AGCCACCCAT GGATCTCAGA CAGTGGTGTC TGGTATGTTC AGATTTGGGT TTTAGAAAAC 
TGCTCTCCTT TACTTACTGT CTTCGGGGCA AGGCGGGGCA CACATACGCA GAAAGTCCCA 
TTGGCAGCCT GTGTGCGTGT CTGTCTTGCA CACGTGTCCC GTGTCCAGCT AGGACCCCCC 
CGGTGGATTT CTTTTTTAGG CCATGTGACA GAAGTCTATC TCGTCCACCT CCACGCCAGC 
GTCTACGCTC TCTTCCATCG CCTGTATGGG ATGTACCCTT GCAACTTTGT CTCCTTTCTG 
CGTTCTCACT ACAGTATGAA GGAAAACCTG GAGACTTTTG AAGAAGTGGT CAAGCCAATG 
ATGGAGCATG TGCGAATTCA CCCGGAATTA GTGACTGGAT CCAAGGACCA TGAACTGGAC 
CCTCGAAGGT GGAAGAGATT AGAAACACAT GATGTTGTCA TAGAATGTGC CAAAATCTCT 
CTGGACCCTA CAGAGGCCTC ATATGAAGAT GGCTATTCTG TGTCTCACCA AATCTCAGCC 
CGTTTTCCTC ACCGTTCAGC CGATGTCTCC GCCAGCTCTT ACGTTGACAC ACAGAATAGC 
TATGGGAATG CTACTTCTAC CCCCTACTCT ACTTCTCGGC TGACGTTAAA CACGCCAGGG 
CAGCTACCTC AGACTCTGAG TTCCCCGTCG ACACGGCTAT TAGCTGAGCC GCCACAAGCT 
ACTCTTTGGA GCCCATCTGC AGTTTGTGGG ATGACCACTC CTCCAACTTC TCCTGGAAAT 
GTCCCACCTG ATCTGTCACA TCCTTACAGT AAAGTCTTTG GTACAACTGC AGGTGGGAAA 
GGAACTCCTC TGGGAACCCC TGCAACCTCT CCTCCTCCAG CCCCACCCTG CCATTCGGAC 
GACTGCGTGC ACCTTTCACT CCCACAGGCC ACGGCCCCCA AGAAGGAAGA GAGGACAGAC 
TCCGCAAGGC CCTGTCTACA CAGACAACCC CATGTTCCGA ATGACAGAGG ATTAGAAGAG 
CTGCACGGAG GCAAGGGATC TGTCACTCTC AGTGATCTTC CAGGGTTTTT AGGAGACCTG 
GCTTCTGAAG AAGACAGTAT TGAGAAGGAT AAAGAAGAAG CGGCCATATC TAAAGAGCTG 
TCTGAGATCA CGACGGCCGA TGCCGAGCCC CTGGTTCCTC GAGGAGGCTT CGACTCACCC 
TTCTACCGGG ACAGTCTGCC GGGCTGCCCG CGGAAGACCC TCTCAGCTTC CTCCAGCACT 
CAGGGCACCA GCGCGAACCC TGAGCCTTTA AACTCCTCCC TGGACAAGTC GGGGCCTGAC 
ACACCAAAGC AAGCCTTCAC GCCCATAGAC CTGCCCTGCG GAGGCACCGG GGAGAGCCCT 
GCCGGGAACA GGGAGCGCCA GGCCTCTCTG GAGGCCAGCA TCCTCACCCC CAGCCCCTGT 
AAAATTCCAC CTCAGAGGGG AGTGGGGTTT GGCAGTGGGC AGCCTCCCCC ATACGATCAT 
CTTTTTGAGG TGGCATTGCC AAAGACTGCC CATCATTTTG TCAGCAAGAA GACGGAGGAA 
CTGCTCAAGA AGGCAAAGGG AAGTGCAGAG GAGGACTTCA CGCCCTCCGC CTCCCCTATG 
GAAGTGCTGG ACAGGCTGAT ACAGCAGGGA GCGGACGTGC ACAGCAAGGA GCTCAACAGG 
TTGTCTTTAC CCAGCAAGTC TGTCGACTGG ACCCACTTCG GAGGCTCTCC TCCCTCAGAT 
GAGATCTGCA CCCTCCGTAA CCAGTTGCTT TTACTGCACA ACCAGTTACT CTACGAGCGT 
TTCAAGCGGC AGCAGCACGC GCTGCGCAAC AGGCGGCTCC TGCGCAAGGT GATCCGGGCC 
TCGGCTCTGG AGGAGCACAA CGCCGCCATG AAAGACCAGT TGAAGTTGCA AGAGAAAGAC 
ATCCAGATAT GGAAGATTAG TCTGCAGAAA GAACAAGCCA GGTACAATCA GCTTCAGGAG 
CAGCGAGACA CAGTGGTCAC CCAGCTTCAT AGCCAGATCA GACAGCTTCA GCACGACCGG 
GAGGAGTTCT ACAACCAGAG CCAGGAGTTA CAGACGAAGC TGGAGGACTG CAGGAACATG 
ATCGCAGAGC TGCGCGTGGA GCTGAAGAAG GCCAACAGCA AGGTGTGCCA CACCGAGCTG 
CTGCTCAGCC AGGTGTCCCA GAAGCTCTCA AATAGTGAGT CGGTCCAACA GCAGATGGAG 
TTCTTGAACA GGCAACTGTT GGTTCTTGGG GAAGTCAACG AGCTGTATTT GGAACAGCTG 
CAGAACAAGC ATTCAGACAC CACCAAGGAA GTAGAAATGA TGAAGGCTGC ATATCGGAAA 
GAGCTAGAAA AAAACAGAAG CCACGTCCTC CAGCAGAATC AGAGGCTTGA TACCTGTCAG 
AAGCGGATTT TGGAACTGGA ATCTCATCTG GCTAAGAAAG ACCACCTTCT TTTAGAACAG 
AAAAAGTATC TAGAGGATGT CAAACTGCAG GCAAGAGGAC AGCTACAGGC TGCAGAGAGC 
AGGTATGAGG CCCAAAAGAG GATCACCCAG GTGTTTGAAT TGGAGATCTT AGATTTATAC 
GGCAAGTTGG AGAAGGATGG CCTCCTGAAA AAACTTGAAG AACAAAAACC AGAAGCAGCT 
GAAGCAGCAG AAGAAAGGCT TGACTACTGT CAGGACGGCT GCTCGGATTC GGTGACAGGG 
CACAACGAAG AGGCCCCTGG CCGTAACGGT GAGACCAAGC CCTCCAGGCC TGGCGGCGCC 
CGGGGCGGTG GCGGTGGCGG CGGCAGAGGT GGCGGCAGCA GCAGCGAGCT CTCCACCCCG 
GAGAAGCCCC CCAACCCCAG GGCGGGCCCG TTCAGCAGTC GGTGGGAGAC GGTGGTGGGT 
GAGCCCACTT CCGGCACCCC CACGACGGTG GGCTCGCTTC CCAGCTCAGA GAGCTTCCTG 
GGCATGAAGG CACGAGAGCT GTTTCGTAAC AAGAGCGAGA GCCAATGCGA CGAGGATGGC 
CTGACTGTTG GCAGCCTTTC TGAGACCCTA AAGACAGAGC TGGGCAAAGA CTCGGGTGTG 
GAAGCCAGGG CCCCCCTGAC CCTTGATGGC CCACTGCTGC CTGCCCCAAA GCCGGACAGC 
GTGGGCCAGC TCCGCATCAT GGACTACAAC GAGGCCCAGC TTGAGCACAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817300.1
CDS	729..3962
Translation	MSARLCGPLQVDGSLSRGQGSHPWISDSGVWYVQIWVLENCSPLLTVFGARRGTHTQKVPLAACVRVCLAHVSRVQLGPPRWISFLGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGGSPPSDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_010818998.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818998.2

ORF Insert Sequence:

ATGAGTGCAC GCCTCTGTGG GCCACTCCAA GTAGATGGGA GTTTATCCAG AGGCCAGGGG 
AGCCACCCAT GGATCTCAGA CAGTGGTGTC TGGTATGTTC AGATTTGGGT TTTAGAAAAC 
TGCTCTCCTT TACTTACTGT CTTCGGGGCA AGGCGGGGCA CACATACGCA GAAAGTCCCA 
TTGGCAGCCT GTGTGCGTGT CTGTCTTGCA CACGTGTCCC GTGTCCAGCT AGGACCCCCC 
CGGTGGATTT CTTTTTTAGG CCATGTGACA GAAGTCTATC TCGTCCACCT CCACGCCAGC 
GTCTACGCTC TCTTCCATCG CCTGTATGGG ATGTACCCTT GCAACTTTGT CTCCTTTCTG 
CGTTCTCACT ACAGTATGAA GGAAAACCTG GAGACTTTTG AAGAAGTGGT CAAGCCAATG 
ATGGAGCATG TGCGAATTCA CCCGGAATTA GTGACTGGAT CCAAGGACCA TGAACTGGAC 
CCTCGAAGGT GGAAGAGATT AGAAACACAT GATGTTGTCA TAGAATGTGC CAAAATCTCT 
CTGGACCCTA CAGAGGCCTC ATATGAAGAT GGCTATTCTG TGTCTCACCA AATCTCAGCC 
CGTTTTCCTC ACCGTTCAGC CGATGTCTCC GCCAGCTCTT ACGTTGACAC ACAGAATAGC 
TATGGGAATG CTACTTCTAC CCCCTACTCT ACTTCTCGGC TGACGTTAAA CACGCCAGGG 
CAGCTACCTC AGACTCTGAG TTCCCCGTCG ACACGGCTAT TAGCTGAGCC GCCACAAGCT 
ACTCTTTGGA GCCCATCTGC AGTTTGTGGG ATGACCACTC CTCCAACTTC TCCTGGAAAT 
GTCCCACCTG ATCTGTCACA TCCTTACAGT AAAGTCTTTG GTACAACTGC AGGTGGGAAA 
GGAACTCCTC TGGGAACCCC TGCAACCTCT CCTCCTCCAG CCCCACCCTG CCATTCGGAC 
GACTGCGTGC ACCTTTCACT CCCACAGGCC ACGGCCCCCA AGAAGGAAGA GAGGACAGAC 
TCCGCAAGGC CCTGTCTACA CAGACAACCC CATGTTCCGA ATGACAGAGG ATTAGAAGAG 
CTGCACGGAG GCAAGGGATC TGTCACTCTC AGTGATCTTC CAGGGTTTTT AGGAGACCTG 
GCTTCTGAAG AAGACAGTAT TGAGAAGGAT AAAGAAGAAG CGGCCATATC TAAAGAGCTG 
TCTGAGATCA CGACGGCCGA TGCCGAGCCC CTGGTTCCTC GAGGAGGCTT CGACTCACCC 
TTCTACCGGG ACAGTCTGCC GGGCTGCCCG CGGAAGACCC TCTCAGCTTC CTCCAGCACT 
CAGGGCACCA GCGCGAACCC TGAGCCTTTA AACTCCTCCC TGGACAAGTC GGGGCCTGAC 
ACACCAAAGC AAGCCTTCAC GCCCATAGAC CTGCCCTGCG GAGGCACCGG GGAGAGCCCT 
GCCGGGAACA GGGAGCGCCA GGCCTCTCTG GAGGCCAGCA TCCTCACCCC CAGCCCCTGT 
AAAATTCCAC CTCAGAGGGG AGTGGGGTTT GGCAGTGGGC AGCCTCCCCC ATACGATCAT 
CTTTTTGAGG TGGCATTGCC AAAGACTGCC CATCATTTTG TCAGCAAGAA GACGGAGGAA 
CTGCTCAAGA AGGCAAAGGG AAGTGCAGAG GAGGACTTCA CGCCCTCCGC CTCCCCTATG 
GAAGTGCTGG ACAGGCTGAT ACAGCAGGGA GCGGACGTGC ACAGCAAGGA GCTCAACAGG 
TTGTCTTTAC CCAGCAAGTC TGTCGACTGG ACCCACTTCG GAGGCTCTCC TCCCTCAGAT 
GAGATCTGCA CCCTCCGTAA CCAGTTGCTT TTACTGCACA ACCAGTTACT CTACGAGCGT 
TTCAAGCGGC AGCAGCACGC GCTGCGCAAC AGGCGGCTCC TGCGCAAGGT GATCCGGGCC 
TCGGCTCTGG AGGAGCACAA CGCCGCCATG AAAGACCAGT TGAAGTTGCA AGAGAAAGAC 
ATCCAGATAT GGAAGATTAG TCTGCAGAAA GAACAAGCCA GGTACAATCA GCTTCAGGAG 
CAGCGAGACA CAGTGGTCAC CCAGCTTCAT AGCCAGATCA GACAGCTTCA GCACGACCGG 
GAGGAGTTCT ACAACCAGAG CCAGGAGTTA CAGACGAAGC TGGAGGACTG CAGGAACATG 
ATCGCAGAGC TGCGCGTGGA GCTGAAGAAG GCCAACAGCA AGGTGTGCCA CACCGAGCTG 
CTGCTCAGCC AGGTGTCCCA GAAGCTCTCA AATAGTGAGT CGGTCCAACA GCAGATGGAG 
TTCTTGAACA GGCAACTGTT GGTTCTTGGG GAAGTCAACG AGCTGTATTT GGAACAGCTG 
CAGAACAAGC ATTCAGACAC CACCAAGGAA GTAGAAATGA TGAAGGCTGC ATATCGGAAA 
GAGCTAGAAA AAAACAGAAG CCACGTCCTC CAGCAGAATC AGAGGCTTGA TACCTGTCAG 
AAGCGGATTT TGGAACTGGA ATCTCATCTG GCTAAGAAAG ACCACCTTCT TTTAGAACAG 
AAAAAGTATC TAGAGGATGT CAAACTGCAG GCAAGAGGAC AGCTACAGGC TGCAGAGAGC 
AGGTATGAGG CCCAAAAGAG GATCACCCAG GTGTTTGAAT TGGAGATCTT AGATTTATAC 
GGCAAGTTGG AGAAGGATGG CCTCCTGAAA AAACTTGAAG AACAAAAACC AGAAGCAGCT 
GAAGCAGCAG AAGAAAGGCT TGACTACTGT CAGGACGGCT GCTCGGATTC GGTGACAGGG 
CACAACGAAG AGGCCCCTGG CCGTAACGGT GAGACCAAGC CCTCCAGGCC TGGCGGCGCC 
CGGGGCGGTG GCGGTGGCGG CGGCAGAGGT GGCGGCAGCA GCAGCGAGCT CTCCACCCCG 
GAGAAGCCCC CCAACCCCAG GGCGGGCCCG TTCAGCAGTC GGTGGGAGAC GGTGGTGGGT 
GAGCCCACTT CCGGCACCCC CACGACGGTG GGCTCGCTTC CCAGCTCAGA GAGCTTCCTG 
GGCATGAAGG CACGAGAGCT GTTTCGTAAC AAGAGCGAGA GCCAATGCGA CGAGGATGGC 
CTGACTGTTG GCAGCCTTTC TGAGACCCTA AAGACAGAGC TGGGCAAAGA CTCGGGTGTG 
GAAGCCAGGG CCCCCCTGAC CCTTGATGGC CCACTGCTGC CTGCCCCAAA GCCGGACAGC 
GTGGGCCAGC TCCGCATCAT GGACTACAAC GAGGCCCAGC TTGAGCACAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_010810530.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808832.1 Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X9, mRNA.

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CloneID	OBa52451
Clone ID Related Accession (Same CDS sequence)	XM_010818998.2 , XM_010810530.2 , XM_010810530.3
Accession Version	XM_010810530.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hamartin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810530.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCAC GCCTCTGTGG GCCACTCCAA GTAGATGGGA GTTTATCCAG AGGCCAGGGG 
AGCCACCCAT GGATCTCAGA CAGTGGTGTC TGGTATGTTC AGATTTGGGT TTTAGAAAAC 
TGCTCTCCTT TACTTACTGT CTTCGGGGCA AGGCGGGGCA CACATACGCA GAAAGTCCCA 
TTGGCAGCCT GTGTGCGTGT CTGTCTTGCA CACGTGTCCC GTGTCCAGCT AGGACCCCCC 
CGGTGGATTT CTTTTTTAGG CCATGTGACA GAAGTCTATC TCGTCCACCT CCACGCCAGC 
GTCTACGCTC TCTTCCATCG CCTGTATGGG ATGTACCCTT GCAACTTTGT CTCCTTTCTG 
CGTTCTCACT ACAGTATGAA GGAAAACCTG GAGACTTTTG AAGAAGTGGT CAAGCCAATG 
ATGGAGCATG TGCGAATTCA CCCGGAATTA GTGACTGGAT CCAAGGACCA TGAACTGGAC 
CCTCGAAGGT GGAAGAGATT AGAAACACAT GATGTTGTCA TAGAATGTGC CAAAATCTCT 
CTGGACCCTA CAGAGGCCTC ATATGAAGAT GGCTATTCTG TGTCTCACCA AATCTCAGCC 
CGTTTTCCTC ACCGTTCAGC CGATGTCTCC GCCAGCTCTT ACGTTGACAC ACAGAATAGC 
TATGGGAATG CTACTTCTAC CCCCTACTCT ACTTCTCGGC TGACGTTAAA CACGCCAGGG 
CAGCTACCTC AGACTCTGAG TTCCCCGTCG ACACGGCTAT TAGCTGAGCC GCCACAAGCT 
ACTCTTTGGA GCCCATCTGC AGTTTGTGGG ATGACCACTC CTCCAACTTC TCCTGGAAAT 
GTCCCACCTG ATCTGTCACA TCCTTACAGT AAAGTCTTTG GTACAACTGC AGGTGGGAAA 
GGAACTCCTC TGGGAACCCC TGCAACCTCT CCTCCTCCAG CCCCACCCTG CCATTCGGAC 
GACTGCGTGC ACCTTTCACT CCCACAGGCC ACGGCCCCCA AGAAGGAAGA GAGGACAGAC 
TCCGCAAGGC CCTGTCTACA CAGACAACCC CATGTTCCGA ATGACAGAGG ATTAGAAGAG 
CTGCACGGAG GCAAGGGATC TGTCACTCTC AGTGATCTTC CAGGGTTTTT AGGAGACCTG 
GCTTCTGAAG AAGACAGTAT TGAGAAGGAT AAAGAAGAAG CGGCCATATC TAAAGAGCTG 
TCTGAGATCA CGACGGCCGA TGCCGAGCCC CTGGTTCCTC GAGGAGGCTT CGACTCACCC 
TTCTACCGGG ACAGTCTGCC GGGCTGCCCG CGGAAGACCC TCTCAGCTTC CTCCAGCACT 
CAGGGCACCA GCGCGAACCC TGAGCCTTTA AACTCCTCCC TGGACAAGTC GGGGCCTGAC 
ACACCAAAGC AAGCCTTCAC GCCCATAGAC CTGCCCTGCG GAGGCACCGG GGAGAGCCCT 
GCCGGGAACA GGGAGCGCCA GGCCTCTCTG GAGGCCAGCA TCCTCACCCC CAGCCCCTGT 
AAAATTCCAC CTCAGAGGGG AGTGGGGTTT GGCAGTGGGC AGCCTCCCCC ATACGATCAT 
CTTTTTGAGG TGGCATTGCC AAAGACTGCC CATCATTTTG TCAGCAAGAA GACGGAGGAA 
CTGCTCAAGA AGGCAAAGGG AAGTGCAGAG GAGGACTTCA CGCCCTCCGC CTCCCCTATG 
GAAGTGCTGG ACAGGCTGAT ACAGCAGGGA GCGGACGTGC ACAGCAAGGA GCTCAACAGG 
TTGTCTTTAC CCAGCAAGTC TGTCGACTGG ACCCACTTCG GAGGCTCTCC TCCCTCAGAT 
GAGATCTGCA CCCTCCGTAA CCAGTTGCTT TTACTGCACA ACCAGTTACT CTACGAGCGT 
TTCAAGCGGC AGCAGCACGC GCTGCGCAAC AGGCGGCTCC TGCGCAAGGT GATCCGGGCC 
TCGGCTCTGG AGGAGCACAA CGCCGCCATG AAAGACCAGT TGAAGTTGCA AGAGAAAGAC 
ATCCAGATAT GGAAGATTAG TCTGCAGAAA GAACAAGCCA GGTACAATCA GCTTCAGGAG 
CAGCGAGACA CAGTGGTCAC CCAGCTTCAT AGCCAGATCA GACAGCTTCA GCACGACCGG 
GAGGAGTTCT ACAACCAGAG CCAGGAGTTA CAGACGAAGC TGGAGGACTG CAGGAACATG 
ATCGCAGAGC TGCGCGTGGA GCTGAAGAAG GCCAACAGCA AGGTGTGCCA CACCGAGCTG 
CTGCTCAGCC AGGTGTCCCA GAAGCTCTCA AATAGTGAGT CGGTCCAACA GCAGATGGAG 
TTCTTGAACA GGCAACTGTT GGTTCTTGGG GAAGTCAACG AGCTGTATTT GGAACAGCTG 
CAGAACAAGC ATTCAGACAC CACCAAGGAA GTAGAAATGA TGAAGGCTGC ATATCGGAAA 
GAGCTAGAAA AAAACAGAAG CCACGTCCTC CAGCAGAATC AGAGGCTTGA TACCTGTCAG 
AAGCGGATTT TGGAACTGGA ATCTCATCTG GCTAAGAAAG ACCACCTTCT TTTAGAACAG 
AAAAAGTATC TAGAGGATGT CAAACTGCAG GCAAGAGGAC AGCTACAGGC TGCAGAGAGC 
AGGTATGAGG CCCAAAAGAG GATCACCCAG GTGTTTGAAT TGGAGATCTT AGATTTATAC 
GGCAAGTTGG AGAAGGATGG CCTCCTGAAA AAACTTGAAG AACAAAAACC AGAAGCAGCT 
GAAGCAGCAG AAGAAAGGCT TGACTACTGT CAGGACGGCT GCTCGGATTC GGTGACAGGG 
CACAACGAAG AGGCCCCTGG CCGTAACGGT GAGACCAAGC CCTCCAGGCC TGGCGGCGCC 
CGGGGCGGTG GCGGTGGCGG CGGCAGAGGT GGCGGCAGCA GCAGCGAGCT CTCCACCCCG 
GAGAAGCCCC CCAACCCCAG GGCGGGCCCG TTCAGCAGTC GGTGGGAGAC GGTGGTGGGT 
GAGCCCACTT CCGGCACCCC CACGACGGTG GGCTCGCTTC CCAGCTCAGA GAGCTTCCTG 
GGCATGAAGG CACGAGAGCT GTTTCGTAAC AAGAGCGAGA GCCAATGCGA CGAGGATGGC 
CTGACTGTTG GCAGCCTTTC TGAGACCCTA AAGACAGAGC TGGGCAAAGA CTCGGGTGTG 
GAAGCCAGGG CCCCCCTGAC CCTTGATGGC CCACTGCTGC CTGCCCCAAA GCCGGACAGC 
GTGGGCCAGC TCCGCATCAT GGACTACAAC GAGGCCCAGC TTGAGCACAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808832.1
CDS	729..3962
Translation	MSARLCGPLQVDGSLSRGQGSHPWISDSGVWYVQIWVLENCSPLLTVFGARRGTHTQKVPLAACVRVCLAHVSRVQLGPPRWISFLGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGGSPPSDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_010810530.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810530.2

ORF Insert Sequence:

ATGAGTGCAC GCCTCTGTGG GCCACTCCAA GTAGATGGGA GTTTATCCAG AGGCCAGGGG 
AGCCACCCAT GGATCTCAGA CAGTGGTGTC TGGTATGTTC AGATTTGGGT TTTAGAAAAC 
TGCTCTCCTT TACTTACTGT CTTCGGGGCA AGGCGGGGCA CACATACGCA GAAAGTCCCA 
TTGGCAGCCT GTGTGCGTGT CTGTCTTGCA CACGTGTCCC GTGTCCAGCT AGGACCCCCC 
CGGTGGATTT CTTTTTTAGG CCATGTGACA GAAGTCTATC TCGTCCACCT CCACGCCAGC 
GTCTACGCTC TCTTCCATCG CCTGTATGGG ATGTACCCTT GCAACTTTGT CTCCTTTCTG 
CGTTCTCACT ACAGTATGAA GGAAAACCTG GAGACTTTTG AAGAAGTGGT CAAGCCAATG 
ATGGAGCATG TGCGAATTCA CCCGGAATTA GTGACTGGAT CCAAGGACCA TGAACTGGAC 
CCTCGAAGGT GGAAGAGATT AGAAACACAT GATGTTGTCA TAGAATGTGC CAAAATCTCT 
CTGGACCCTA CAGAGGCCTC ATATGAAGAT GGCTATTCTG TGTCTCACCA AATCTCAGCC 
CGTTTTCCTC ACCGTTCAGC CGATGTCTCC GCCAGCTCTT ACGTTGACAC ACAGAATAGC 
TATGGGAATG CTACTTCTAC CCCCTACTCT ACTTCTCGGC TGACGTTAAA CACGCCAGGG 
CAGCTACCTC AGACTCTGAG TTCCCCGTCG ACACGGCTAT TAGCTGAGCC GCCACAAGCT 
ACTCTTTGGA GCCCATCTGC AGTTTGTGGG ATGACCACTC CTCCAACTTC TCCTGGAAAT 
GTCCCACCTG ATCTGTCACA TCCTTACAGT AAAGTCTTTG GTACAACTGC AGGTGGGAAA 
GGAACTCCTC TGGGAACCCC TGCAACCTCT CCTCCTCCAG CCCCACCCTG CCATTCGGAC 
GACTGCGTGC ACCTTTCACT CCCACAGGCC ACGGCCCCCA AGAAGGAAGA GAGGACAGAC 
TCCGCAAGGC CCTGTCTACA CAGACAACCC CATGTTCCGA ATGACAGAGG ATTAGAAGAG 
CTGCACGGAG GCAAGGGATC TGTCACTCTC AGTGATCTTC CAGGGTTTTT AGGAGACCTG 
GCTTCTGAAG AAGACAGTAT TGAGAAGGAT AAAGAAGAAG CGGCCATATC TAAAGAGCTG 
TCTGAGATCA CGACGGCCGA TGCCGAGCCC CTGGTTCCTC GAGGAGGCTT CGACTCACCC 
TTCTACCGGG ACAGTCTGCC GGGCTGCCCG CGGAAGACCC TCTCAGCTTC CTCCAGCACT 
CAGGGCACCA GCGCGAACCC TGAGCCTTTA AACTCCTCCC TGGACAAGTC GGGGCCTGAC 
ACACCAAAGC AAGCCTTCAC GCCCATAGAC CTGCCCTGCG GAGGCACCGG GGAGAGCCCT 
GCCGGGAACA GGGAGCGCCA GGCCTCTCTG GAGGCCAGCA TCCTCACCCC CAGCCCCTGT 
AAAATTCCAC CTCAGAGGGG AGTGGGGTTT GGCAGTGGGC AGCCTCCCCC ATACGATCAT 
CTTTTTGAGG TGGCATTGCC AAAGACTGCC CATCATTTTG TCAGCAAGAA GACGGAGGAA 
CTGCTCAAGA AGGCAAAGGG AAGTGCAGAG GAGGACTTCA CGCCCTCCGC CTCCCCTATG 
GAAGTGCTGG ACAGGCTGAT ACAGCAGGGA GCGGACGTGC ACAGCAAGGA GCTCAACAGG 
TTGTCTTTAC CCAGCAAGTC TGTCGACTGG ACCCACTTCG GAGGCTCTCC TCCCTCAGAT 
GAGATCTGCA CCCTCCGTAA CCAGTTGCTT TTACTGCACA ACCAGTTACT CTACGAGCGT 
TTCAAGCGGC AGCAGCACGC GCTGCGCAAC AGGCGGCTCC TGCGCAAGGT GATCCGGGCC 
TCGGCTCTGG AGGAGCACAA CGCCGCCATG AAAGACCAGT TGAAGTTGCA AGAGAAAGAC 
ATCCAGATAT GGAAGATTAG TCTGCAGAAA GAACAAGCCA GGTACAATCA GCTTCAGGAG 
CAGCGAGACA CAGTGGTCAC CCAGCTTCAT AGCCAGATCA GACAGCTTCA GCACGACCGG 
GAGGAGTTCT ACAACCAGAG CCAGGAGTTA CAGACGAAGC TGGAGGACTG CAGGAACATG 
ATCGCAGAGC TGCGCGTGGA GCTGAAGAAG GCCAACAGCA AGGTGTGCCA CACCGAGCTG 
CTGCTCAGCC AGGTGTCCCA GAAGCTCTCA AATAGTGAGT CGGTCCAACA GCAGATGGAG 
TTCTTGAACA GGCAACTGTT GGTTCTTGGG GAAGTCAACG AGCTGTATTT GGAACAGCTG 
CAGAACAAGC ATTCAGACAC CACCAAGGAA GTAGAAATGA TGAAGGCTGC ATATCGGAAA 
GAGCTAGAAA AAAACAGAAG CCACGTCCTC CAGCAGAATC AGAGGCTTGA TACCTGTCAG 
AAGCGGATTT TGGAACTGGA ATCTCATCTG GCTAAGAAAG ACCACCTTCT TTTAGAACAG 
AAAAAGTATC TAGAGGATGT CAAACTGCAG GCAAGAGGAC AGCTACAGGC TGCAGAGAGC 
AGGTATGAGG CCCAAAAGAG GATCACCCAG GTGTTTGAAT TGGAGATCTT AGATTTATAC 
GGCAAGTTGG AGAAGGATGG CCTCCTGAAA AAACTTGAAG AACAAAAACC AGAAGCAGCT 
GAAGCAGCAG AAGAAAGGCT TGACTACTGT CAGGACGGCT GCTCGGATTC GGTGACAGGG 
CACAACGAAG AGGCCCCTGG CCGTAACGGT GAGACCAAGC CCTCCAGGCC TGGCGGCGCC 
CGGGGCGGTG GCGGTGGCGG CGGCAGAGGT GGCGGCAGCA GCAGCGAGCT CTCCACCCCG 
GAGAAGCCCC CCAACCCCAG GGCGGGCCCG TTCAGCAGTC GGTGGGAGAC GGTGGTGGGT 
GAGCCCACTT CCGGCACCCC CACGACGGTG GGCTCGCTTC CCAGCTCAGA GAGCTTCCTG 
GGCATGAAGG CACGAGAGCT GTTTCGTAAC AAGAGCGAGA GCCAATGCGA CGAGGATGGC 
CTGACTGTTG GCAGCCTTTC TGAGACCCTA AAGACAGAGC TGGGCAAAGA CTCGGGTGTG 
GAAGCCAGGG CCCCCCTGAC CCTTGATGGC CCACTGCTGC CTGCCCCAAA GCCGGACAGC 
GTGGGCCAGC TCCGCATCAT GGACTACAAC GAGGCCCAGC TTGAGCACAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_010810530.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808832.1 Bos taurus TSC complex subunit 1 (TSC1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa52451
Clone ID Related Accession (Same CDS sequence)	XM_010818998.2 , XM_010810530.2 , XM_010810530.3
Accession Version	XM_010810530.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	hamartin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810530.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCAC GCCTCTGTGG GCCACTCCAA GTAGATGGGA GTTTATCCAG AGGCCAGGGG 
AGCCACCCAT GGATCTCAGA CAGTGGTGTC TGGTATGTTC AGATTTGGGT TTTAGAAAAC 
TGCTCTCCTT TACTTACTGT CTTCGGGGCA AGGCGGGGCA CACATACGCA GAAAGTCCCA 
TTGGCAGCCT GTGTGCGTGT CTGTCTTGCA CACGTGTCCC GTGTCCAGCT AGGACCCCCC 
CGGTGGATTT CTTTTTTAGG CCATGTGACA GAAGTCTATC TCGTCCACCT CCACGCCAGC 
GTCTACGCTC TCTTCCATCG CCTGTATGGG ATGTACCCTT GCAACTTTGT CTCCTTTCTG 
CGTTCTCACT ACAGTATGAA GGAAAACCTG GAGACTTTTG AAGAAGTGGT CAAGCCAATG 
ATGGAGCATG TGCGAATTCA CCCGGAATTA GTGACTGGAT CCAAGGACCA TGAACTGGAC 
CCTCGAAGGT GGAAGAGATT AGAAACACAT GATGTTGTCA TAGAATGTGC CAAAATCTCT 
CTGGACCCTA CAGAGGCCTC ATATGAAGAT GGCTATTCTG TGTCTCACCA AATCTCAGCC 
CGTTTTCCTC ACCGTTCAGC CGATGTCTCC GCCAGCTCTT ACGTTGACAC ACAGAATAGC 
TATGGGAATG CTACTTCTAC CCCCTACTCT ACTTCTCGGC TGACGTTAAA CACGCCAGGG 
CAGCTACCTC AGACTCTGAG TTCCCCGTCG ACACGGCTAT TAGCTGAGCC GCCACAAGCT 
ACTCTTTGGA GCCCATCTGC AGTTTGTGGG ATGACCACTC CTCCAACTTC TCCTGGAAAT 
GTCCCACCTG ATCTGTCACA TCCTTACAGT AAAGTCTTTG GTACAACTGC AGGTGGGAAA 
GGAACTCCTC TGGGAACCCC TGCAACCTCT CCTCCTCCAG CCCCACCCTG CCATTCGGAC 
GACTGCGTGC ACCTTTCACT CCCACAGGCC ACGGCCCCCA AGAAGGAAGA GAGGACAGAC 
TCCGCAAGGC CCTGTCTACA CAGACAACCC CATGTTCCGA ATGACAGAGG ATTAGAAGAG 
CTGCACGGAG GCAAGGGATC TGTCACTCTC AGTGATCTTC CAGGGTTTTT AGGAGACCTG 
GCTTCTGAAG AAGACAGTAT TGAGAAGGAT AAAGAAGAAG CGGCCATATC TAAAGAGCTG 
TCTGAGATCA CGACGGCCGA TGCCGAGCCC CTGGTTCCTC GAGGAGGCTT CGACTCACCC 
TTCTACCGGG ACAGTCTGCC GGGCTGCCCG CGGAAGACCC TCTCAGCTTC CTCCAGCACT 
CAGGGCACCA GCGCGAACCC TGAGCCTTTA AACTCCTCCC TGGACAAGTC GGGGCCTGAC 
ACACCAAAGC AAGCCTTCAC GCCCATAGAC CTGCCCTGCG GAGGCACCGG GGAGAGCCCT 
GCCGGGAACA GGGAGCGCCA GGCCTCTCTG GAGGCCAGCA TCCTCACCCC CAGCCCCTGT 
AAAATTCCAC CTCAGAGGGG AGTGGGGTTT GGCAGTGGGC AGCCTCCCCC ATACGATCAT 
CTTTTTGAGG TGGCATTGCC AAAGACTGCC CATCATTTTG TCAGCAAGAA GACGGAGGAA 
CTGCTCAAGA AGGCAAAGGG AAGTGCAGAG GAGGACTTCA CGCCCTCCGC CTCCCCTATG 
GAAGTGCTGG ACAGGCTGAT ACAGCAGGGA GCGGACGTGC ACAGCAAGGA GCTCAACAGG 
TTGTCTTTAC CCAGCAAGTC TGTCGACTGG ACCCACTTCG GAGGCTCTCC TCCCTCAGAT 
GAGATCTGCA CCCTCCGTAA CCAGTTGCTT TTACTGCACA ACCAGTTACT CTACGAGCGT 
TTCAAGCGGC AGCAGCACGC GCTGCGCAAC AGGCGGCTCC TGCGCAAGGT GATCCGGGCC 
TCGGCTCTGG AGGAGCACAA CGCCGCCATG AAAGACCAGT TGAAGTTGCA AGAGAAAGAC 
ATCCAGATAT GGAAGATTAG TCTGCAGAAA GAACAAGCCA GGTACAATCA GCTTCAGGAG 
CAGCGAGACA CAGTGGTCAC CCAGCTTCAT AGCCAGATCA GACAGCTTCA GCACGACCGG 
GAGGAGTTCT ACAACCAGAG CCAGGAGTTA CAGACGAAGC TGGAGGACTG CAGGAACATG 
ATCGCAGAGC TGCGCGTGGA GCTGAAGAAG GCCAACAGCA AGGTGTGCCA CACCGAGCTG 
CTGCTCAGCC AGGTGTCCCA GAAGCTCTCA AATAGTGAGT CGGTCCAACA GCAGATGGAG 
TTCTTGAACA GGCAACTGTT GGTTCTTGGG GAAGTCAACG AGCTGTATTT GGAACAGCTG 
CAGAACAAGC ATTCAGACAC CACCAAGGAA GTAGAAATGA TGAAGGCTGC ATATCGGAAA 
GAGCTAGAAA AAAACAGAAG CCACGTCCTC CAGCAGAATC AGAGGCTTGA TACCTGTCAG 
AAGCGGATTT TGGAACTGGA ATCTCATCTG GCTAAGAAAG ACCACCTTCT TTTAGAACAG 
AAAAAGTATC TAGAGGATGT CAAACTGCAG GCAAGAGGAC AGCTACAGGC TGCAGAGAGC 
AGGTATGAGG CCCAAAAGAG GATCACCCAG GTGTTTGAAT TGGAGATCTT AGATTTATAC 
GGCAAGTTGG AGAAGGATGG CCTCCTGAAA AAACTTGAAG AACAAAAACC AGAAGCAGCT 
GAAGCAGCAG AAGAAAGGCT TGACTACTGT CAGGACGGCT GCTCGGATTC GGTGACAGGG 
CACAACGAAG AGGCCCCTGG CCGTAACGGT GAGACCAAGC CCTCCAGGCC TGGCGGCGCC 
CGGGGCGGTG GCGGTGGCGG CGGCAGAGGT GGCGGCAGCA GCAGCGAGCT CTCCACCCCG 
GAGAAGCCCC CCAACCCCAG GGCGGGCCCG TTCAGCAGTC GGTGGGAGAC GGTGGTGGGT 
GAGCCCACTT CCGGCACCCC CACGACGGTG GGCTCGCTTC CCAGCTCAGA GAGCTTCCTG 
GGCATGAAGG CACGAGAGCT GTTTCGTAAC AAGAGCGAGA GCCAATGCGA CGAGGATGGC 
CTGACTGTTG GCAGCCTTTC TGAGACCCTA AAGACAGAGC TGGGCAAAGA CTCGGGTGTG 
GAAGCCAGGG CCCCCCTGAC CCTTGATGGC CCACTGCTGC CTGCCCCAAA GCCGGACAGC 
GTGGGCCAGC TCCGCATCAT GGACTACAAC GAGGCCCAGC TTGAGCACAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808832.1
CDS	762..3995
Translation	MSARLCGPLQVDGSLSRGQGSHPWISDSGVWYVQIWVLENCSPLLTVFGARRGTHTQKVPLAACVRVCLAHVSRVQLGPPRWISFLGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGGSPPSDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_010810530.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TSC complex subunit 1 (TSC1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810530.3

ORF Insert Sequence:

ATGAGTGCAC GCCTCTGTGG GCCACTCCAA GTAGATGGGA GTTTATCCAG AGGCCAGGGG 
AGCCACCCAT GGATCTCAGA CAGTGGTGTC TGGTATGTTC AGATTTGGGT TTTAGAAAAC 
TGCTCTCCTT TACTTACTGT CTTCGGGGCA AGGCGGGGCA CACATACGCA GAAAGTCCCA 
TTGGCAGCCT GTGTGCGTGT CTGTCTTGCA CACGTGTCCC GTGTCCAGCT AGGACCCCCC 
CGGTGGATTT CTTTTTTAGG CCATGTGACA GAAGTCTATC TCGTCCACCT CCACGCCAGC 
GTCTACGCTC TCTTCCATCG CCTGTATGGG ATGTACCCTT GCAACTTTGT CTCCTTTCTG 
CGTTCTCACT ACAGTATGAA GGAAAACCTG GAGACTTTTG AAGAAGTGGT CAAGCCAATG 
ATGGAGCATG TGCGAATTCA CCCGGAATTA GTGACTGGAT CCAAGGACCA TGAACTGGAC 
CCTCGAAGGT GGAAGAGATT AGAAACACAT GATGTTGTCA TAGAATGTGC CAAAATCTCT 
CTGGACCCTA CAGAGGCCTC ATATGAAGAT GGCTATTCTG TGTCTCACCA AATCTCAGCC 
CGTTTTCCTC ACCGTTCAGC CGATGTCTCC GCCAGCTCTT ACGTTGACAC ACAGAATAGC 
TATGGGAATG CTACTTCTAC CCCCTACTCT ACTTCTCGGC TGACGTTAAA CACGCCAGGG 
CAGCTACCTC AGACTCTGAG TTCCCCGTCG ACACGGCTAT TAGCTGAGCC GCCACAAGCT 
ACTCTTTGGA GCCCATCTGC AGTTTGTGGG ATGACCACTC CTCCAACTTC TCCTGGAAAT 
GTCCCACCTG ATCTGTCACA TCCTTACAGT AAAGTCTTTG GTACAACTGC AGGTGGGAAA 
GGAACTCCTC TGGGAACCCC TGCAACCTCT CCTCCTCCAG CCCCACCCTG CCATTCGGAC 
GACTGCGTGC ACCTTTCACT CCCACAGGCC ACGGCCCCCA AGAAGGAAGA GAGGACAGAC 
TCCGCAAGGC CCTGTCTACA CAGACAACCC CATGTTCCGA ATGACAGAGG ATTAGAAGAG 
CTGCACGGAG GCAAGGGATC TGTCACTCTC AGTGATCTTC CAGGGTTTTT AGGAGACCTG 
GCTTCTGAAG AAGACAGTAT TGAGAAGGAT AAAGAAGAAG CGGCCATATC TAAAGAGCTG 
TCTGAGATCA CGACGGCCGA TGCCGAGCCC CTGGTTCCTC GAGGAGGCTT CGACTCACCC 
TTCTACCGGG ACAGTCTGCC GGGCTGCCCG CGGAAGACCC TCTCAGCTTC CTCCAGCACT 
CAGGGCACCA GCGCGAACCC TGAGCCTTTA AACTCCTCCC TGGACAAGTC GGGGCCTGAC 
ACACCAAAGC AAGCCTTCAC GCCCATAGAC CTGCCCTGCG GAGGCACCGG GGAGAGCCCT 
GCCGGGAACA GGGAGCGCCA GGCCTCTCTG GAGGCCAGCA TCCTCACCCC CAGCCCCTGT 
AAAATTCCAC CTCAGAGGGG AGTGGGGTTT GGCAGTGGGC AGCCTCCCCC ATACGATCAT 
CTTTTTGAGG TGGCATTGCC AAAGACTGCC CATCATTTTG TCAGCAAGAA GACGGAGGAA 
CTGCTCAAGA AGGCAAAGGG AAGTGCAGAG GAGGACTTCA CGCCCTCCGC CTCCCCTATG 
GAAGTGCTGG ACAGGCTGAT ACAGCAGGGA GCGGACGTGC ACAGCAAGGA GCTCAACAGG 
TTGTCTTTAC CCAGCAAGTC TGTCGACTGG ACCCACTTCG GAGGCTCTCC TCCCTCAGAT 
GAGATCTGCA CCCTCCGTAA CCAGTTGCTT TTACTGCACA ACCAGTTACT CTACGAGCGT 
TTCAAGCGGC AGCAGCACGC GCTGCGCAAC AGGCGGCTCC TGCGCAAGGT GATCCGGGCC 
TCGGCTCTGG AGGAGCACAA CGCCGCCATG AAAGACCAGT TGAAGTTGCA AGAGAAAGAC 
ATCCAGATAT GGAAGATTAG TCTGCAGAAA GAACAAGCCA GGTACAATCA GCTTCAGGAG 
CAGCGAGACA CAGTGGTCAC CCAGCTTCAT AGCCAGATCA GACAGCTTCA GCACGACCGG 
GAGGAGTTCT ACAACCAGAG CCAGGAGTTA CAGACGAAGC TGGAGGACTG CAGGAACATG 
ATCGCAGAGC TGCGCGTGGA GCTGAAGAAG GCCAACAGCA AGGTGTGCCA CACCGAGCTG 
CTGCTCAGCC AGGTGTCCCA GAAGCTCTCA AATAGTGAGT CGGTCCAACA GCAGATGGAG 
TTCTTGAACA GGCAACTGTT GGTTCTTGGG GAAGTCAACG AGCTGTATTT GGAACAGCTG 
CAGAACAAGC ATTCAGACAC CACCAAGGAA GTAGAAATGA TGAAGGCTGC ATATCGGAAA 
GAGCTAGAAA AAAACAGAAG CCACGTCCTC CAGCAGAATC AGAGGCTTGA TACCTGTCAG 
AAGCGGATTT TGGAACTGGA ATCTCATCTG GCTAAGAAAG ACCACCTTCT TTTAGAACAG 
AAAAAGTATC TAGAGGATGT CAAACTGCAG GCAAGAGGAC AGCTACAGGC TGCAGAGAGC 
AGGTATGAGG CCCAAAAGAG GATCACCCAG GTGTTTGAAT TGGAGATCTT AGATTTATAC 
GGCAAGTTGG AGAAGGATGG CCTCCTGAAA AAACTTGAAG AACAAAAACC AGAAGCAGCT 
GAAGCAGCAG AAGAAAGGCT TGACTACTGT CAGGACGGCT GCTCGGATTC GGTGACAGGG 
CACAACGAAG AGGCCCCTGG CCGTAACGGT GAGACCAAGC CCTCCAGGCC TGGCGGCGCC 
CGGGGCGGTG GCGGTGGCGG CGGCAGAGGT GGCGGCAGCA GCAGCGAGCT CTCCACCCCG 
GAGAAGCCCC CCAACCCCAG GGCGGGCCCG TTCAGCAGTC GGTGGGAGAC GGTGGTGGGT 
GAGCCCACTT CCGGCACCCC CACGACGGTG GGCTCGCTTC CCAGCTCAGA GAGCTTCCTG 
GGCATGAAGG CACGAGAGCT GTTTCGTAAC AAGAGCGAGA GCCAATGCGA CGAGGATGGC 
CTGACTGTTG GCAGCCTTTC TGAGACCCTA AAGACAGAGC TGGGCAAAGA CTCGGGTGTG 
GAAGCCAGGG CCCCCCTGAC CCTTGATGGC CCACTGCTGC CTGCCCCAAA GCCGGACAGC 
GTGGGCCAGC TCCGCATCAT GGACTACAAC GAGGCCCAGC TTGAGCACAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_024999615.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855383.1 Bos taurus TSC complex subunit 1 (TSC1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa52452
Clone ID Related Accession (Same CDS sequence)	XM_024999615.1 , XM_010810531.2 , XM_010819002.2
Accession Version	XM_024999615.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	hamartin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACCG ACGTCATTGT CCTCACAACA GGAGTCTTAG TGTTGATAAC CATGCTCCCA 
ATGATTCCGC AGTCGGGGAA ACAGCACCTT CATGATTTCT TTGATATCTT TGGCCGTCTT 
TCGTCATGGT GCCTGAAGAA ACCAGGCCAT GTGACAGAAG TCTATCTCGT CCACCTCCAC 
GCCAGCGTCT ACGCTCTCTT CCATCGCCTG TATGGGATGT ACCCTTGCAA CTTTGTCTCC 
TTTCTGCGTT CTCACTACAG TATGAAGGAA AACCTGGAGA CTTTTGAAGA AGTGGTCAAG 
CCAATGATGG AGCATGTGCG AATTCACCCG GAATTAGTGA CTGGATCCAA GGACCATGAA 
CTGGACCCTC GAAGGTGGAA GAGATTAGAA ACACATGATG TTGTCATAGA ATGTGCCAAA 
ATCTCTCTGG ACCCTACAGA GGCCTCATAT GAAGATGGCT ATTCTGTGTC TCACCAAATC 
TCAGCCCGTT TTCCTCACCG TTCAGCCGAT GTCTCCGCCA GCTCTTACGT TGACACACAG 
AATAGCTATG GGAATGCTAC TTCTACCCCC TACTCTACTT CTCGGCTGAC GTTAAACACG 
CCAGGGCAGC TACCTCAGAC TCTGAGTTCC CCGTCGACAC GGCTATTAGC TGAGCCGCCA 
CAAGCTACTC TTTGGAGCCC ATCTGCAGTT TGTGGGATGA CCACTCCTCC AACTTCTCCT 
GGAAATGTCC CACCTGATCT GTCACATCCT TACAGTAAAG TCTTTGGTAC AACTGCAGGT 
GGGAAAGGAA CTCCTCTGGG AACCCCTGCA ACCTCTCCTC CTCCAGCCCC ACCCTGCCAT 
TCGGACGACT GCGTGCACCT TTCACTCCCA CAGGCCACGG CCCCCAAGAA GGAAGAGAGG 
ACAGACTCCG CAAGGCCCTG TCTACACAGA CAACCCCATG TTCCGAATGA CAGAGGATTA 
GAAGAGCTGC ACGGAGGCAA GGGATCTGTC ACTCTCAGTG ATCTTCCAGG GTTTTTAGGA 
GACCTGGCTT CTGAAGAAGA CAGTATTGAG AAGGATAAAG AAGAAGCGGC CATATCTAAA 
GAGCTGTCTG AGATCACGAC GGCCGATGCC GAGCCCCTGG TTCCTCGAGG AGGCTTCGAC 
TCACCCTTCT ACCGGGACAG TCTGCCGGGC TGCCCGCGGA AGACCCTCTC AGCTTCCTCC 
AGCACTCAGG GCACCAGCGC GAACCCTGAG CCTTTAAACT CCTCCCTGGA CAAGTCGGGG 
CCTGACACAC CAAAGCAAGC CTTCACGCCC ATAGACCTGC CCTGCGGAGG CACCGGGGAG 
AGCCCTGCCG GGAACAGGGA GCGCCAGGCC TCTCTGGAGG CCAGCATCCT CACCCCCAGC 
CCCTGTAAAA TTCCACCTCA GAGGGGAGTG GGGTTTGGCA GTGGGCAGCC TCCCCCATAC 
GATCATCTTT TTGAGGTGGC ATTGCCAAAG ACTGCCCATC ATTTTGTCAG CAAGAAGACG 
GAGGAACTGC TCAAGAAGGC AAAGGGAAGT GCAGAGGAGG ACTTCACGCC CTCCGCCTCC 
CCTATGGAAG TGCTGGACAG GCTGATACAG CAGGGAGCGG ACGTGCACAG CAAGGAGCTC 
AACAGGTTGT CTTTACCCAG CAAGTCTGTC GACTGGACCC ACTTCGGAGG CTCTCCTCCC 
TCAGATGAGA TCTGCACCCT CCGTAACCAG TTGCTTTTAC TGCACAACCA GTTACTCTAC 
GAGCGTTTCA AGCGGCAGCA GCACGCGCTG CGCAACAGGC GGCTCCTGCG CAAGGTGATC 
CGGGCCTCGG CTCTGGAGGA GCACAACGCC GCCATGAAAG ACCAGTTGAA GTTGCAAGAG 
AAAGACATCC AGATATGGAA GATTAGTCTG CAGAAAGAAC AAGCCAGGTA CAATCAGCTT 
CAGGAGCAGC GAGACACAGT GGTCACCCAG CTTCATAGCC AGATCAGACA GCTTCAGCAC 
GACCGGGAGG AGTTCTACAA CCAGAGCCAG GAGTTACAGA CGAAGCTGGA GGACTGCAGG 
AACATGATCG CAGAGCTGCG CGTGGAGCTG AAGAAGGCCA ACAGCAAGGT GTGCCACACC 
GAGCTGCTGC TCAGCCAGGT GTCCCAGAAG CTCTCAAATA GTGAGTCGGT CCAACAGCAG 
ATGGAGTTCT TGAACAGGCA ACTGTTGGTT CTTGGGGAAG TCAACGAGCT GTATTTGGAA 
CAGCTGCAGA ACAAGCATTC AGACACCACC AAGGAAGTAG AAATGATGAA GGCTGCATAT 
CGGAAAGAGC TAGAAAAAAA CAGAAGCCAC GTCCTCCAGC AGAATCAGAG GCTTGATACC 
TGTCAGAAGC GGATTTTGGA ACTGGAATCT CATCTGGCTA AGAAAGACCA CCTTCTTTTA 
GAACAGAAAA AGTATCTAGA GGATGTCAAA CTGCAGGCAA GAGGACAGCT ACAGGCTGCA 
GAGAGCAGGT ATGAGGCCCA AAAGAGGATC ACCCAGGTGT TTGAATTGGA GATCTTAGAT 
TTATACGGCA AGTTGGAGAA GGATGGCCTC CTGAAAAAAC TTGAAGAACA AAAACCAGAA 
GCAGCTGAAG CAGCAGAAGA AAGGCTTGAC TACTGTCAGG ACGGCTGCTC GGATTCGGTG 
ACAGGGCACA ACGAAGAGGC CCCTGGCCGT AACGGTGAGA CCAAGCCCTC CAGGCCTGGC 
GGCGCCCGGG GCGGTGGCGG TGGCGGCGGC AGAGGTGGCG GCAGCAGCAG CGAGCTCTCC 
ACCCCGGAGA AGCCCCCCAA CCCCAGGGCG GGCCCGTTCA GCAGTCGGTG GGAGACGGTG 
GTGGGTGAGC CCACTTCCGG CACCCCCACG ACGGTGGGCT CGCTTCCCAG CTCAGAGAGC 
TTCCTGGGCA TGAAGGCACG AGAGCTGTTT CGTAACAAGA GCGAGAGCCA ATGCGACGAG 
GATGGCCTGA CTGTTGGCAG CCTTTCTGAG ACCCTAAAGA CAGAGCTGGG CAAAGACTCG 
GGTGTGGAAG CCAGGGCCCC CCTGACCCTT GATGGCCCAC TGCTGCCTGC CCCAAAGCCG 
GACAGCGTGG GCCAGCTCCG CATCATGGAC TACAACGAGG CCCAGCTTGA GCACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855383.1
CDS	905..4024
Translation	MDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGGSPPSDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_024999615.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TSC complex subunit 1 (TSC1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999615.1

ORF Insert Sequence:

ATGGACACCG ACGTCATTGT CCTCACAACA GGAGTCTTAG TGTTGATAAC CATGCTCCCA 
ATGATTCCGC AGTCGGGGAA ACAGCACCTT CATGATTTCT TTGATATCTT TGGCCGTCTT 
TCGTCATGGT GCCTGAAGAA ACCAGGCCAT GTGACAGAAG TCTATCTCGT CCACCTCCAC 
GCCAGCGTCT ACGCTCTCTT CCATCGCCTG TATGGGATGT ACCCTTGCAA CTTTGTCTCC 
TTTCTGCGTT CTCACTACAG TATGAAGGAA AACCTGGAGA CTTTTGAAGA AGTGGTCAAG 
CCAATGATGG AGCATGTGCG AATTCACCCG GAATTAGTGA CTGGATCCAA GGACCATGAA 
CTGGACCCTC GAAGGTGGAA GAGATTAGAA ACACATGATG TTGTCATAGA ATGTGCCAAA 
ATCTCTCTGG ACCCTACAGA GGCCTCATAT GAAGATGGCT ATTCTGTGTC TCACCAAATC 
TCAGCCCGTT TTCCTCACCG TTCAGCCGAT GTCTCCGCCA GCTCTTACGT TGACACACAG 
AATAGCTATG GGAATGCTAC TTCTACCCCC TACTCTACTT CTCGGCTGAC GTTAAACACG 
CCAGGGCAGC TACCTCAGAC TCTGAGTTCC CCGTCGACAC GGCTATTAGC TGAGCCGCCA 
CAAGCTACTC TTTGGAGCCC ATCTGCAGTT TGTGGGATGA CCACTCCTCC AACTTCTCCT 
GGAAATGTCC CACCTGATCT GTCACATCCT TACAGTAAAG TCTTTGGTAC AACTGCAGGT 
GGGAAAGGAA CTCCTCTGGG AACCCCTGCA ACCTCTCCTC CTCCAGCCCC ACCCTGCCAT 
TCGGACGACT GCGTGCACCT TTCACTCCCA CAGGCCACGG CCCCCAAGAA GGAAGAGAGG 
ACAGACTCCG CAAGGCCCTG TCTACACAGA CAACCCCATG TTCCGAATGA CAGAGGATTA 
GAAGAGCTGC ACGGAGGCAA GGGATCTGTC ACTCTCAGTG ATCTTCCAGG GTTTTTAGGA 
GACCTGGCTT CTGAAGAAGA CAGTATTGAG AAGGATAAAG AAGAAGCGGC CATATCTAAA 
GAGCTGTCTG AGATCACGAC GGCCGATGCC GAGCCCCTGG TTCCTCGAGG AGGCTTCGAC 
TCACCCTTCT ACCGGGACAG TCTGCCGGGC TGCCCGCGGA AGACCCTCTC AGCTTCCTCC 
AGCACTCAGG GCACCAGCGC GAACCCTGAG CCTTTAAACT CCTCCCTGGA CAAGTCGGGG 
CCTGACACAC CAAAGCAAGC CTTCACGCCC ATAGACCTGC CCTGCGGAGG CACCGGGGAG 
AGCCCTGCCG GGAACAGGGA GCGCCAGGCC TCTCTGGAGG CCAGCATCCT CACCCCCAGC 
CCCTGTAAAA TTCCACCTCA GAGGGGAGTG GGGTTTGGCA GTGGGCAGCC TCCCCCATAC 
GATCATCTTT TTGAGGTGGC ATTGCCAAAG ACTGCCCATC ATTTTGTCAG CAAGAAGACG 
GAGGAACTGC TCAAGAAGGC AAAGGGAAGT GCAGAGGAGG ACTTCACGCC CTCCGCCTCC 
CCTATGGAAG TGCTGGACAG GCTGATACAG CAGGGAGCGG ACGTGCACAG CAAGGAGCTC 
AACAGGTTGT CTTTACCCAG CAAGTCTGTC GACTGGACCC ACTTCGGAGG CTCTCCTCCC 
TCAGATGAGA TCTGCACCCT CCGTAACCAG TTGCTTTTAC TGCACAACCA GTTACTCTAC 
GAGCGTTTCA AGCGGCAGCA GCACGCGCTG CGCAACAGGC GGCTCCTGCG CAAGGTGATC 
CGGGCCTCGG CTCTGGAGGA GCACAACGCC GCCATGAAAG ACCAGTTGAA GTTGCAAGAG 
AAAGACATCC AGATATGGAA GATTAGTCTG CAGAAAGAAC AAGCCAGGTA CAATCAGCTT 
CAGGAGCAGC GAGACACAGT GGTCACCCAG CTTCATAGCC AGATCAGACA GCTTCAGCAC 
GACCGGGAGG AGTTCTACAA CCAGAGCCAG GAGTTACAGA CGAAGCTGGA GGACTGCAGG 
AACATGATCG CAGAGCTGCG CGTGGAGCTG AAGAAGGCCA ACAGCAAGGT GTGCCACACC 
GAGCTGCTGC TCAGCCAGGT GTCCCAGAAG CTCTCAAATA GTGAGTCGGT CCAACAGCAG 
ATGGAGTTCT TGAACAGGCA ACTGTTGGTT CTTGGGGAAG TCAACGAGCT GTATTTGGAA 
CAGCTGCAGA ACAAGCATTC AGACACCACC AAGGAAGTAG AAATGATGAA GGCTGCATAT 
CGGAAAGAGC TAGAAAAAAA CAGAAGCCAC GTCCTCCAGC AGAATCAGAG GCTTGATACC 
TGTCAGAAGC GGATTTTGGA ACTGGAATCT CATCTGGCTA AGAAAGACCA CCTTCTTTTA 
GAACAGAAAA AGTATCTAGA GGATGTCAAA CTGCAGGCAA GAGGACAGCT ACAGGCTGCA 
GAGAGCAGGT ATGAGGCCCA AAAGAGGATC ACCCAGGTGT TTGAATTGGA GATCTTAGAT 
TTATACGGCA AGTTGGAGAA GGATGGCCTC CTGAAAAAAC TTGAAGAACA AAAACCAGAA 
GCAGCTGAAG CAGCAGAAGA AAGGCTTGAC TACTGTCAGG ACGGCTGCTC GGATTCGGTG 
ACAGGGCACA ACGAAGAGGC CCCTGGCCGT AACGGTGAGA CCAAGCCCTC CAGGCCTGGC 
GGCGCCCGGG GCGGTGGCGG TGGCGGCGGC AGAGGTGGCG GCAGCAGCAG CGAGCTCTCC 
ACCCCGGAGA AGCCCCCCAA CCCCAGGGCG GGCCCGTTCA GCAGTCGGTG GGAGACGGTG 
GTGGGTGAGC CCACTTCCGG CACCCCCACG ACGGTGGGCT CGCTTCCCAG CTCAGAGAGC 
TTCCTGGGCA TGAAGGCACG AGAGCTGTTT CGTAACAAGA GCGAGAGCCA ATGCGACGAG 
GATGGCCTGA CTGTTGGCAG CCTTTCTGAG ACCCTAAAGA CAGAGCTGGG CAAAGACTCG 
GGTGTGGAAG CCAGGGCCCC CCTGACCCTT GATGGCCCAC TGCTGCCTGC CCCAAAGCCG 
GACAGCGTGG GCCAGCTCCG CATCATGGAC TACAACGAGG CCCAGCTTGA GCACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_010810531.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808833.1 Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa52452
Clone ID Related Accession (Same CDS sequence)	XM_024999615.1 , XM_010810531.2 , XM_010819002.2
Accession Version	XM_010810531.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hamartin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810531.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACCG ACGTCATTGT CCTCACAACA GGAGTCTTAG TGTTGATAAC CATGCTCCCA 
ATGATTCCGC AGTCGGGGAA ACAGCACCTT CATGATTTCT TTGATATCTT TGGCCGTCTT 
TCGTCATGGT GCCTGAAGAA ACCAGGCCAT GTGACAGAAG TCTATCTCGT CCACCTCCAC 
GCCAGCGTCT ACGCTCTCTT CCATCGCCTG TATGGGATGT ACCCTTGCAA CTTTGTCTCC 
TTTCTGCGTT CTCACTACAG TATGAAGGAA AACCTGGAGA CTTTTGAAGA AGTGGTCAAG 
CCAATGATGG AGCATGTGCG AATTCACCCG GAATTAGTGA CTGGATCCAA GGACCATGAA 
CTGGACCCTC GAAGGTGGAA GAGATTAGAA ACACATGATG TTGTCATAGA ATGTGCCAAA 
ATCTCTCTGG ACCCTACAGA GGCCTCATAT GAAGATGGCT ATTCTGTGTC TCACCAAATC 
TCAGCCCGTT TTCCTCACCG TTCAGCCGAT GTCTCCGCCA GCTCTTACGT TGACACACAG 
AATAGCTATG GGAATGCTAC TTCTACCCCC TACTCTACTT CTCGGCTGAC GTTAAACACG 
CCAGGGCAGC TACCTCAGAC TCTGAGTTCC CCGTCGACAC GGCTATTAGC TGAGCCGCCA 
CAAGCTACTC TTTGGAGCCC ATCTGCAGTT TGTGGGATGA CCACTCCTCC AACTTCTCCT 
GGAAATGTCC CACCTGATCT GTCACATCCT TACAGTAAAG TCTTTGGTAC AACTGCAGGT 
GGGAAAGGAA CTCCTCTGGG AACCCCTGCA ACCTCTCCTC CTCCAGCCCC ACCCTGCCAT 
TCGGACGACT GCGTGCACCT TTCACTCCCA CAGGCCACGG CCCCCAAGAA GGAAGAGAGG 
ACAGACTCCG CAAGGCCCTG TCTACACAGA CAACCCCATG TTCCGAATGA CAGAGGATTA 
GAAGAGCTGC ACGGAGGCAA GGGATCTGTC ACTCTCAGTG ATCTTCCAGG GTTTTTAGGA 
GACCTGGCTT CTGAAGAAGA CAGTATTGAG AAGGATAAAG AAGAAGCGGC CATATCTAAA 
GAGCTGTCTG AGATCACGAC GGCCGATGCC GAGCCCCTGG TTCCTCGAGG AGGCTTCGAC 
TCACCCTTCT ACCGGGACAG TCTGCCGGGC TGCCCGCGGA AGACCCTCTC AGCTTCCTCC 
AGCACTCAGG GCACCAGCGC GAACCCTGAG CCTTTAAACT CCTCCCTGGA CAAGTCGGGG 
CCTGACACAC CAAAGCAAGC CTTCACGCCC ATAGACCTGC CCTGCGGAGG CACCGGGGAG 
AGCCCTGCCG GGAACAGGGA GCGCCAGGCC TCTCTGGAGG CCAGCATCCT CACCCCCAGC 
CCCTGTAAAA TTCCACCTCA GAGGGGAGTG GGGTTTGGCA GTGGGCAGCC TCCCCCATAC 
GATCATCTTT TTGAGGTGGC ATTGCCAAAG ACTGCCCATC ATTTTGTCAG CAAGAAGACG 
GAGGAACTGC TCAAGAAGGC AAAGGGAAGT GCAGAGGAGG ACTTCACGCC CTCCGCCTCC 
CCTATGGAAG TGCTGGACAG GCTGATACAG CAGGGAGCGG ACGTGCACAG CAAGGAGCTC 
AACAGGTTGT CTTTACCCAG CAAGTCTGTC GACTGGACCC ACTTCGGAGG CTCTCCTCCC 
TCAGATGAGA TCTGCACCCT CCGTAACCAG TTGCTTTTAC TGCACAACCA GTTACTCTAC 
GAGCGTTTCA AGCGGCAGCA GCACGCGCTG CGCAACAGGC GGCTCCTGCG CAAGGTGATC 
CGGGCCTCGG CTCTGGAGGA GCACAACGCC GCCATGAAAG ACCAGTTGAA GTTGCAAGAG 
AAAGACATCC AGATATGGAA GATTAGTCTG CAGAAAGAAC AAGCCAGGTA CAATCAGCTT 
CAGGAGCAGC GAGACACAGT GGTCACCCAG CTTCATAGCC AGATCAGACA GCTTCAGCAC 
GACCGGGAGG AGTTCTACAA CCAGAGCCAG GAGTTACAGA CGAAGCTGGA GGACTGCAGG 
AACATGATCG CAGAGCTGCG CGTGGAGCTG AAGAAGGCCA ACAGCAAGGT GTGCCACACC 
GAGCTGCTGC TCAGCCAGGT GTCCCAGAAG CTCTCAAATA GTGAGTCGGT CCAACAGCAG 
ATGGAGTTCT TGAACAGGCA ACTGTTGGTT CTTGGGGAAG TCAACGAGCT GTATTTGGAA 
CAGCTGCAGA ACAAGCATTC AGACACCACC AAGGAAGTAG AAATGATGAA GGCTGCATAT 
CGGAAAGAGC TAGAAAAAAA CAGAAGCCAC GTCCTCCAGC AGAATCAGAG GCTTGATACC 
TGTCAGAAGC GGATTTTGGA ACTGGAATCT CATCTGGCTA AGAAAGACCA CCTTCTTTTA 
GAACAGAAAA AGTATCTAGA GGATGTCAAA CTGCAGGCAA GAGGACAGCT ACAGGCTGCA 
GAGAGCAGGT ATGAGGCCCA AAAGAGGATC ACCCAGGTGT TTGAATTGGA GATCTTAGAT 
TTATACGGCA AGTTGGAGAA GGATGGCCTC CTGAAAAAAC TTGAAGAACA AAAACCAGAA 
GCAGCTGAAG CAGCAGAAGA AAGGCTTGAC TACTGTCAGG ACGGCTGCTC GGATTCGGTG 
ACAGGGCACA ACGAAGAGGC CCCTGGCCGT AACGGTGAGA CCAAGCCCTC CAGGCCTGGC 
GGCGCCCGGG GCGGTGGCGG TGGCGGCGGC AGAGGTGGCG GCAGCAGCAG CGAGCTCTCC 
ACCCCGGAGA AGCCCCCCAA CCCCAGGGCG GGCCCGTTCA GCAGTCGGTG GGAGACGGTG 
GTGGGTGAGC CCACTTCCGG CACCCCCACG ACGGTGGGCT CGCTTCCCAG CTCAGAGAGC 
TTCCTGGGCA TGAAGGCACG AGAGCTGTTT CGTAACAAGA GCGAGAGCCA ATGCGACGAG 
GATGGCCTGA CTGTTGGCAG CCTTTCTGAG ACCCTAAAGA CAGAGCTGGG CAAAGACTCG 
GGTGTGGAAG CCAGGGCCCC CCTGACCCTT GATGGCCCAC TGCTGCCTGC CCCAAAGCCG 
GACAGCGTGG GCCAGCTCCG CATCATGGAC TACAACGAGG CCCAGCTTGA GCACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808833.1
CDS	299..3418
Translation	MDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGGSPPSDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_010810531.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810531.2

ORF Insert Sequence:

ATGGACACCG ACGTCATTGT CCTCACAACA GGAGTCTTAG TGTTGATAAC CATGCTCCCA 
ATGATTCCGC AGTCGGGGAA ACAGCACCTT CATGATTTCT TTGATATCTT TGGCCGTCTT 
TCGTCATGGT GCCTGAAGAA ACCAGGCCAT GTGACAGAAG TCTATCTCGT CCACCTCCAC 
GCCAGCGTCT ACGCTCTCTT CCATCGCCTG TATGGGATGT ACCCTTGCAA CTTTGTCTCC 
TTTCTGCGTT CTCACTACAG TATGAAGGAA AACCTGGAGA CTTTTGAAGA AGTGGTCAAG 
CCAATGATGG AGCATGTGCG AATTCACCCG GAATTAGTGA CTGGATCCAA GGACCATGAA 
CTGGACCCTC GAAGGTGGAA GAGATTAGAA ACACATGATG TTGTCATAGA ATGTGCCAAA 
ATCTCTCTGG ACCCTACAGA GGCCTCATAT GAAGATGGCT ATTCTGTGTC TCACCAAATC 
TCAGCCCGTT TTCCTCACCG TTCAGCCGAT GTCTCCGCCA GCTCTTACGT TGACACACAG 
AATAGCTATG GGAATGCTAC TTCTACCCCC TACTCTACTT CTCGGCTGAC GTTAAACACG 
CCAGGGCAGC TACCTCAGAC TCTGAGTTCC CCGTCGACAC GGCTATTAGC TGAGCCGCCA 
CAAGCTACTC TTTGGAGCCC ATCTGCAGTT TGTGGGATGA CCACTCCTCC AACTTCTCCT 
GGAAATGTCC CACCTGATCT GTCACATCCT TACAGTAAAG TCTTTGGTAC AACTGCAGGT 
GGGAAAGGAA CTCCTCTGGG AACCCCTGCA ACCTCTCCTC CTCCAGCCCC ACCCTGCCAT 
TCGGACGACT GCGTGCACCT TTCACTCCCA CAGGCCACGG CCCCCAAGAA GGAAGAGAGG 
ACAGACTCCG CAAGGCCCTG TCTACACAGA CAACCCCATG TTCCGAATGA CAGAGGATTA 
GAAGAGCTGC ACGGAGGCAA GGGATCTGTC ACTCTCAGTG ATCTTCCAGG GTTTTTAGGA 
GACCTGGCTT CTGAAGAAGA CAGTATTGAG AAGGATAAAG AAGAAGCGGC CATATCTAAA 
GAGCTGTCTG AGATCACGAC GGCCGATGCC GAGCCCCTGG TTCCTCGAGG AGGCTTCGAC 
TCACCCTTCT ACCGGGACAG TCTGCCGGGC TGCCCGCGGA AGACCCTCTC AGCTTCCTCC 
AGCACTCAGG GCACCAGCGC GAACCCTGAG CCTTTAAACT CCTCCCTGGA CAAGTCGGGG 
CCTGACACAC CAAAGCAAGC CTTCACGCCC ATAGACCTGC CCTGCGGAGG CACCGGGGAG 
AGCCCTGCCG GGAACAGGGA GCGCCAGGCC TCTCTGGAGG CCAGCATCCT CACCCCCAGC 
CCCTGTAAAA TTCCACCTCA GAGGGGAGTG GGGTTTGGCA GTGGGCAGCC TCCCCCATAC 
GATCATCTTT TTGAGGTGGC ATTGCCAAAG ACTGCCCATC ATTTTGTCAG CAAGAAGACG 
GAGGAACTGC TCAAGAAGGC AAAGGGAAGT GCAGAGGAGG ACTTCACGCC CTCCGCCTCC 
CCTATGGAAG TGCTGGACAG GCTGATACAG CAGGGAGCGG ACGTGCACAG CAAGGAGCTC 
AACAGGTTGT CTTTACCCAG CAAGTCTGTC GACTGGACCC ACTTCGGAGG CTCTCCTCCC 
TCAGATGAGA TCTGCACCCT CCGTAACCAG TTGCTTTTAC TGCACAACCA GTTACTCTAC 
GAGCGTTTCA AGCGGCAGCA GCACGCGCTG CGCAACAGGC GGCTCCTGCG CAAGGTGATC 
CGGGCCTCGG CTCTGGAGGA GCACAACGCC GCCATGAAAG ACCAGTTGAA GTTGCAAGAG 
AAAGACATCC AGATATGGAA GATTAGTCTG CAGAAAGAAC AAGCCAGGTA CAATCAGCTT 
CAGGAGCAGC GAGACACAGT GGTCACCCAG CTTCATAGCC AGATCAGACA GCTTCAGCAC 
GACCGGGAGG AGTTCTACAA CCAGAGCCAG GAGTTACAGA CGAAGCTGGA GGACTGCAGG 
AACATGATCG CAGAGCTGCG CGTGGAGCTG AAGAAGGCCA ACAGCAAGGT GTGCCACACC 
GAGCTGCTGC TCAGCCAGGT GTCCCAGAAG CTCTCAAATA GTGAGTCGGT CCAACAGCAG 
ATGGAGTTCT TGAACAGGCA ACTGTTGGTT CTTGGGGAAG TCAACGAGCT GTATTTGGAA 
CAGCTGCAGA ACAAGCATTC AGACACCACC AAGGAAGTAG AAATGATGAA GGCTGCATAT 
CGGAAAGAGC TAGAAAAAAA CAGAAGCCAC GTCCTCCAGC AGAATCAGAG GCTTGATACC 
TGTCAGAAGC GGATTTTGGA ACTGGAATCT CATCTGGCTA AGAAAGACCA CCTTCTTTTA 
GAACAGAAAA AGTATCTAGA GGATGTCAAA CTGCAGGCAA GAGGACAGCT ACAGGCTGCA 
GAGAGCAGGT ATGAGGCCCA AAAGAGGATC ACCCAGGTGT TTGAATTGGA GATCTTAGAT 
TTATACGGCA AGTTGGAGAA GGATGGCCTC CTGAAAAAAC TTGAAGAACA AAAACCAGAA 
GCAGCTGAAG CAGCAGAAGA AAGGCTTGAC TACTGTCAGG ACGGCTGCTC GGATTCGGTG 
ACAGGGCACA ACGAAGAGGC CCCTGGCCGT AACGGTGAGA CCAAGCCCTC CAGGCCTGGC 
GGCGCCCGGG GCGGTGGCGG TGGCGGCGGC AGAGGTGGCG GCAGCAGCAG CGAGCTCTCC 
ACCCCGGAGA AGCCCCCCAA CCCCAGGGCG GGCCCGTTCA GCAGTCGGTG GGAGACGGTG 
GTGGGTGAGC CCACTTCCGG CACCCCCACG ACGGTGGGCT CGCTTCCCAG CTCAGAGAGC 
TTCCTGGGCA TGAAGGCACG AGAGCTGTTT CGTAACAAGA GCGAGAGCCA ATGCGACGAG 
GATGGCCTGA CTGTTGGCAG CCTTTCTGAG ACCCTAAAGA CAGAGCTGGG CAAAGACTCG 
GGTGTGGAAG CCAGGGCCCC CCTGACCCTT GATGGCCCAC TGCTGCCTGC CCCAAAGCCG 
GACAGCGTGG GCCAGCTCCG CATCATGGAC TACAACGAGG CCCAGCTTGA GCACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_010819002.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817304.1 Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X10, mRNA.

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CloneID	OBa52452
Clone ID Related Accession (Same CDS sequence)	XM_024999615.1 , XM_010810531.2 , XM_010819002.2
Accession Version	XM_010819002.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hamartin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819002.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACCG ACGTCATTGT CCTCACAACA GGAGTCTTAG TGTTGATAAC CATGCTCCCA 
ATGATTCCGC AGTCGGGGAA ACAGCACCTT CATGATTTCT TTGATATCTT TGGCCGTCTT 
TCGTCATGGT GCCTGAAGAA ACCAGGCCAT GTGACAGAAG TCTATCTCGT CCACCTCCAC 
GCCAGCGTCT ACGCTCTCTT CCATCGCCTG TATGGGATGT ACCCTTGCAA CTTTGTCTCC 
TTTCTGCGTT CTCACTACAG TATGAAGGAA AACCTGGAGA CTTTTGAAGA AGTGGTCAAG 
CCAATGATGG AGCATGTGCG AATTCACCCG GAATTAGTGA CTGGATCCAA GGACCATGAA 
CTGGACCCTC GAAGGTGGAA GAGATTAGAA ACACATGATG TTGTCATAGA ATGTGCCAAA 
ATCTCTCTGG ACCCTACAGA GGCCTCATAT GAAGATGGCT ATTCTGTGTC TCACCAAATC 
TCAGCCCGTT TTCCTCACCG TTCAGCCGAT GTCTCCGCCA GCTCTTACGT TGACACACAG 
AATAGCTATG GGAATGCTAC TTCTACCCCC TACTCTACTT CTCGGCTGAC GTTAAACACG 
CCAGGGCAGC TACCTCAGAC TCTGAGTTCC CCGTCGACAC GGCTATTAGC TGAGCCGCCA 
CAAGCTACTC TTTGGAGCCC ATCTGCAGTT TGTGGGATGA CCACTCCTCC AACTTCTCCT 
GGAAATGTCC CACCTGATCT GTCACATCCT TACAGTAAAG TCTTTGGTAC AACTGCAGGT 
GGGAAAGGAA CTCCTCTGGG AACCCCTGCA ACCTCTCCTC CTCCAGCCCC ACCCTGCCAT 
TCGGACGACT GCGTGCACCT TTCACTCCCA CAGGCCACGG CCCCCAAGAA GGAAGAGAGG 
ACAGACTCCG CAAGGCCCTG TCTACACAGA CAACCCCATG TTCCGAATGA CAGAGGATTA 
GAAGAGCTGC ACGGAGGCAA GGGATCTGTC ACTCTCAGTG ATCTTCCAGG GTTTTTAGGA 
GACCTGGCTT CTGAAGAAGA CAGTATTGAG AAGGATAAAG AAGAAGCGGC CATATCTAAA 
GAGCTGTCTG AGATCACGAC GGCCGATGCC GAGCCCCTGG TTCCTCGAGG AGGCTTCGAC 
TCACCCTTCT ACCGGGACAG TCTGCCGGGC TGCCCGCGGA AGACCCTCTC AGCTTCCTCC 
AGCACTCAGG GCACCAGCGC GAACCCTGAG CCTTTAAACT CCTCCCTGGA CAAGTCGGGG 
CCTGACACAC CAAAGCAAGC CTTCACGCCC ATAGACCTGC CCTGCGGAGG CACCGGGGAG 
AGCCCTGCCG GGAACAGGGA GCGCCAGGCC TCTCTGGAGG CCAGCATCCT CACCCCCAGC 
CCCTGTAAAA TTCCACCTCA GAGGGGAGTG GGGTTTGGCA GTGGGCAGCC TCCCCCATAC 
GATCATCTTT TTGAGGTGGC ATTGCCAAAG ACTGCCCATC ATTTTGTCAG CAAGAAGACG 
GAGGAACTGC TCAAGAAGGC AAAGGGAAGT GCAGAGGAGG ACTTCACGCC CTCCGCCTCC 
CCTATGGAAG TGCTGGACAG GCTGATACAG CAGGGAGCGG ACGTGCACAG CAAGGAGCTC 
AACAGGTTGT CTTTACCCAG CAAGTCTGTC GACTGGACCC ACTTCGGAGG CTCTCCTCCC 
TCAGATGAGA TCTGCACCCT CCGTAACCAG TTGCTTTTAC TGCACAACCA GTTACTCTAC 
GAGCGTTTCA AGCGGCAGCA GCACGCGCTG CGCAACAGGC GGCTCCTGCG CAAGGTGATC 
CGGGCCTCGG CTCTGGAGGA GCACAACGCC GCCATGAAAG ACCAGTTGAA GTTGCAAGAG 
AAAGACATCC AGATATGGAA GATTAGTCTG CAGAAAGAAC AAGCCAGGTA CAATCAGCTT 
CAGGAGCAGC GAGACACAGT GGTCACCCAG CTTCATAGCC AGATCAGACA GCTTCAGCAC 
GACCGGGAGG AGTTCTACAA CCAGAGCCAG GAGTTACAGA CGAAGCTGGA GGACTGCAGG 
AACATGATCG CAGAGCTGCG CGTGGAGCTG AAGAAGGCCA ACAGCAAGGT GTGCCACACC 
GAGCTGCTGC TCAGCCAGGT GTCCCAGAAG CTCTCAAATA GTGAGTCGGT CCAACAGCAG 
ATGGAGTTCT TGAACAGGCA ACTGTTGGTT CTTGGGGAAG TCAACGAGCT GTATTTGGAA 
CAGCTGCAGA ACAAGCATTC AGACACCACC AAGGAAGTAG AAATGATGAA GGCTGCATAT 
CGGAAAGAGC TAGAAAAAAA CAGAAGCCAC GTCCTCCAGC AGAATCAGAG GCTTGATACC 
TGTCAGAAGC GGATTTTGGA ACTGGAATCT CATCTGGCTA AGAAAGACCA CCTTCTTTTA 
GAACAGAAAA AGTATCTAGA GGATGTCAAA CTGCAGGCAA GAGGACAGCT ACAGGCTGCA 
GAGAGCAGGT ATGAGGCCCA AAAGAGGATC ACCCAGGTGT TTGAATTGGA GATCTTAGAT 
TTATACGGCA AGTTGGAGAA GGATGGCCTC CTGAAAAAAC TTGAAGAACA AAAACCAGAA 
GCAGCTGAAG CAGCAGAAGA AAGGCTTGAC TACTGTCAGG ACGGCTGCTC GGATTCGGTG 
ACAGGGCACA ACGAAGAGGC CCCTGGCCGT AACGGTGAGA CCAAGCCCTC CAGGCCTGGC 
GGCGCCCGGG GCGGTGGCGG TGGCGGCGGC AGAGGTGGCG GCAGCAGCAG CGAGCTCTCC 
ACCCCGGAGA AGCCCCCCAA CCCCAGGGCG GGCCCGTTCA GCAGTCGGTG GGAGACGGTG 
GTGGGTGAGC CCACTTCCGG CACCCCCACG ACGGTGGGCT CGCTTCCCAG CTCAGAGAGC 
TTCCTGGGCA TGAAGGCACG AGAGCTGTTT CGTAACAAGA GCGAGAGCCA ATGCGACGAG 
GATGGCCTGA CTGTTGGCAG CCTTTCTGAG ACCCTAAAGA CAGAGCTGGG CAAAGACTCG 
GGTGTGGAAG CCAGGGCCCC CCTGACCCTT GATGGCCCAC TGCTGCCTGC CCCAAAGCCG 
GACAGCGTGG GCCAGCTCCG CATCATGGAC TACAACGAGG CCCAGCTTGA GCACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817304.1
CDS	299..3418
Translation	MDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVSASSYVDTQNSYGNATSTPYSTSRLTLNTPGQLPQTLSSPSTRLLAEPPQATLWSPSAVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPPCHSDDCVHLSLPQATAPKKEERTDSARPCLHRQPHVPNDRGLEELHGGKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLVPRGGFDSPFYRDSLPGCPRKTLSASSSTQGTSANPEPLNSSLDKSGPDTPKQAFTPIDLPCGGTGESPAGNRERQASLEASILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTEELLKKAKGSAEEDFTPSASPMEVLDRLIQQGADVHSKELNRLSLPSKSVDWTHFGGSPPSDEICTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRASALEEHNAAMKDQLKLQEKDIQIWKISLQKEQARYNQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQNQRLDTCQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGKLEKDGLLKKLEEQKPEAAEAAEERLDYCQDGCSDSVTGHNEEAPGRNGETKPSRPGGARGGGGGGGRGGGSSSELSTPEKPPNPRAGPFSSRWETVVGEPTSGTPTTVGSLPSSESFLGMKARELFRNKSESQCDEDGLTVGSLSETLKTELGKDSGVEARAPLTLDGPLLPAPKPDSVGQLRIMDYNEAQLEHS

Target ORF information:

RefSeq Version	XM_010819002.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 1 (TSC1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819002.2

ORF Insert Sequence:

ATGGACACCG ACGTCATTGT CCTCACAACA GGAGTCTTAG TGTTGATAAC CATGCTCCCA 
ATGATTCCGC AGTCGGGGAA ACAGCACCTT CATGATTTCT TTGATATCTT TGGCCGTCTT 
TCGTCATGGT GCCTGAAGAA ACCAGGCCAT GTGACAGAAG TCTATCTCGT CCACCTCCAC 
GCCAGCGTCT ACGCTCTCTT CCATCGCCTG TATGGGATGT ACCCTTGCAA CTTTGTCTCC 
TTTCTGCGTT CTCACTACAG TATGAAGGAA AACCTGGAGA CTTTTGAAGA AGTGGTCAAG 
CCAATGATGG AGCATGTGCG AATTCACCCG GAATTAGTGA CTGGATCCAA GGACCATGAA 
CTGGACCCTC GAAGGTGGAA GAGATTAGAA ACACATGATG TTGTCATAGA ATGTGCCAAA 
ATCTCTCTGG ACCCTACAGA GGCCTCATAT GAAGATGGCT ATTCTGTGTC TCACCAAATC 
TCAGCCCGTT TTCCTCACCG TTCAGCCGAT GTCTCCGCCA GCTCTTACGT TGACACACAG 
AATAGCTATG GGAATGCTAC TTCTACCCCC TACTCTACTT CTCGGCTGAC GTTAAACACG 
CCAGGGCAGC TACCTCAGAC TCTGAGTTCC CCGTCGACAC GGCTATTAGC TGAGCCGCCA 
CAAGCTACTC TTTGGAGCCC ATCTGCAGTT TGTGGGATGA CCACTCCTCC AACTTCTCCT 
GGAAATGTCC CACCTGATCT GTCACATCCT TACAGTAAAG TCTTTGGTAC AACTGCAGGT 
GGGAAAGGAA CTCCTCTGGG AACCCCTGCA ACCTCTCCTC CTCCAGCCCC ACCCTGCCAT 
TCGGACGACT GCGTGCACCT TTCACTCCCA CAGGCCACGG CCCCCAAGAA GGAAGAGAGG 
ACAGACTCCG CAAGGCCCTG TCTACACAGA CAACCCCATG TTCCGAATGA CAGAGGATTA 
GAAGAGCTGC ACGGAGGCAA GGGATCTGTC ACTCTCAGTG ATCTTCCAGG GTTTTTAGGA 
GACCTGGCTT CTGAAGAAGA CAGTATTGAG AAGGATAAAG AAGAAGCGGC CATATCTAAA 
GAGCTGTCTG AGATCACGAC GGCCGATGCC GAGCCCCTGG TTCCTCGAGG AGGCTTCGAC 
TCACCCTTCT ACCGGGACAG TCTGCCGGGC TGCCCGCGGA AGACCCTCTC AGCTTCCTCC 
AGCACTCAGG GCACCAGCGC GAACCCTGAG CCTTTAAACT CCTCCCTGGA CAAGTCGGGG 
CCTGACACAC CAAAGCAAGC CTTCACGCCC ATAGACCTGC CCTGCGGAGG CACCGGGGAG 
AGCCCTGCCG GGAACAGGGA GCGCCAGGCC TCTCTGGAGG CCAGCATCCT CACCCCCAGC 
CCCTGTAAAA TTCCACCTCA GAGGGGAGTG GGGTTTGGCA GTGGGCAGCC TCCCCCATAC 
GATCATCTTT TTGAGGTGGC ATTGCCAAAG ACTGCCCATC ATTTTGTCAG CAAGAAGACG 
GAGGAACTGC TCAAGAAGGC AAAGGGAAGT GCAGAGGAGG ACTTCACGCC CTCCGCCTCC 
CCTATGGAAG TGCTGGACAG GCTGATACAG CAGGGAGCGG ACGTGCACAG CAAGGAGCTC 
AACAGGTTGT CTTTACCCAG CAAGTCTGTC GACTGGACCC ACTTCGGAGG CTCTCCTCCC 
TCAGATGAGA TCTGCACCCT CCGTAACCAG TTGCTTTTAC TGCACAACCA GTTACTCTAC 
GAGCGTTTCA AGCGGCAGCA GCACGCGCTG CGCAACAGGC GGCTCCTGCG CAAGGTGATC 
CGGGCCTCGG CTCTGGAGGA GCACAACGCC GCCATGAAAG ACCAGTTGAA GTTGCAAGAG 
AAAGACATCC AGATATGGAA GATTAGTCTG CAGAAAGAAC AAGCCAGGTA CAATCAGCTT 
CAGGAGCAGC GAGACACAGT GGTCACCCAG CTTCATAGCC AGATCAGACA GCTTCAGCAC 
GACCGGGAGG AGTTCTACAA CCAGAGCCAG GAGTTACAGA CGAAGCTGGA GGACTGCAGG 
AACATGATCG CAGAGCTGCG CGTGGAGCTG AAGAAGGCCA ACAGCAAGGT GTGCCACACC 
GAGCTGCTGC TCAGCCAGGT GTCCCAGAAG CTCTCAAATA GTGAGTCGGT CCAACAGCAG 
ATGGAGTTCT TGAACAGGCA ACTGTTGGTT CTTGGGGAAG TCAACGAGCT GTATTTGGAA 
CAGCTGCAGA ACAAGCATTC AGACACCACC AAGGAAGTAG AAATGATGAA GGCTGCATAT 
CGGAAAGAGC TAGAAAAAAA CAGAAGCCAC GTCCTCCAGC AGAATCAGAG GCTTGATACC 
TGTCAGAAGC GGATTTTGGA ACTGGAATCT CATCTGGCTA AGAAAGACCA CCTTCTTTTA 
GAACAGAAAA AGTATCTAGA GGATGTCAAA CTGCAGGCAA GAGGACAGCT ACAGGCTGCA 
GAGAGCAGGT ATGAGGCCCA AAAGAGGATC ACCCAGGTGT TTGAATTGGA GATCTTAGAT 
TTATACGGCA AGTTGGAGAA GGATGGCCTC CTGAAAAAAC TTGAAGAACA AAAACCAGAA 
GCAGCTGAAG CAGCAGAAGA AAGGCTTGAC TACTGTCAGG ACGGCTGCTC GGATTCGGTG 
ACAGGGCACA ACGAAGAGGC CCCTGGCCGT AACGGTGAGA CCAAGCCCTC CAGGCCTGGC 
GGCGCCCGGG GCGGTGGCGG TGGCGGCGGC AGAGGTGGCG GCAGCAGCAG CGAGCTCTCC 
ACCCCGGAGA AGCCCCCCAA CCCCAGGGCG GGCCCGTTCA GCAGTCGGTG GGAGACGGTG 
GTGGGTGAGC CCACTTCCGG CACCCCCACG ACGGTGGGCT CGCTTCCCAG CTCAGAGAGC 
TTCCTGGGCA TGAAGGCACG AGAGCTGTTT CGTAACAAGA GCGAGAGCCA ATGCGACGAG 
GATGGCCTGA CTGTTGGCAG CCTTTCTGAG ACCCTAAAGA CAGAGCTGGG CAAAGACTCG 
GGTGTGGAAG CCAGGGCCCC CCTGACCCTT GATGGCCCAC TGCTGCCTGC CCCAAAGCCG 
GACAGCGTGG GCCAGCTCCG CATCATGGAC TACAACGAGG CCCAGCTTGA GCACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TSC1
Entrez Gene ID	533440
Full Name	TSC complex subunit 1
General protein information	Preferred Names TSC complex subunit 1 Names hamartin tuberous sclerosis 1 protein
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	TSC1	NP_000359.1
Bos taurus (cattle)	TSC1	XP_005213508.1
	TSC1	XP_001103846.2
Mus musculus (house mouse)	Tsc1	NP_075025.2
Xenopus tropicalis (tropical clawed frog)	tsc1	XP_002944681.2
Pan troglodytes (chimpanzee)	TSC1	XP_001168992.1
Gallus gallus (chicken)	TSC1	XP_415449.2
Canis lupus familiaris (dog)	TSC1	XP_005625233.1
Rattus norvegicus (Norway rat)	Tsc1	NP_068626.1
Danio rerio (zebrafish)	tsc1a	NP_956346.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TSC1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: