TSC1 gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_015279760.1	XP_015135246.1	hamartin isoform X1
XM_004945922.2	XP_004945979.1	hamartin isoform X2
XM_015279761.1	XP_015135247.1	hamartin isoform X4
XM_004945922.3	XP_004945979.1	hamartin isoform X2
XM_004945923.3	XP_004945980.1	hamartin isoform X3
XM_004945923.2	XP_004945980.1	hamartin isoform X3
XM_415449.6	XP_415449.2	hamartin isoform X1
XM_015279761.2	XP_015135247.1	hamartin isoform X4
XM_415449.5	XP_415449.2	hamartin isoform X1
XM_015279760.2	XP_015135246.1	hamartin isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa43675	XM_015279761.2 Latest version!	Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OGa43672	XM_015279760.2 Latest version!	Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa43672	XM_415449.6 Latest version!	Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa43673	XM_004945922.3 Latest version!	Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa43674	XM_004945923.3 Latest version!	Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OGa43673	XM_004945922.2	Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa43674	XM_004945923.2	Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa43675	XM_015279761.1	Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OGa43672	XM_415449.5	Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa43672	XM_015279760.1	Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

TSC1 ( XM_015279761.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135247.1 Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OGa43675
Clone ID Related Accession (Same CDS sequence)	XM_015279761.1 , XM_015279761.2
Accession Version	XM_015279761.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	hamartin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAAG GTCACGTGGC AGAGATTTAC CTTGTCCATC TTCATGCCAG TGTTTATGCT 
CTCTTTCATC GGCTTTATGG AATGTATCCT TGCAATTTTG TCTCCTTTCT GCGTTCTCAC 
TACAGTATGA AGGAAAACTT GGAGACCTTT GAAGAGGTAG TCAAGCCAAT GATGGAACAT 
GTGCGAATTC ATCCAGAATT AGTGACTGGA TCTAAGGACC ATGAACTGGA CCCACGAAGG 
TGGAAAAGAC TAGAAACTCA TGATGTTGTG ATAGAATGTG CCAAAATCTC CCTGGACCCT 
GCAGAAGCCT CATATGAAGA TGGCTATTAC TCTGCGTCTC GGAAATCCTG TACAAACTTA 
AAACATCATC AAACTGACCC CAGTGCCAGC CATTACATTG ACACACAGAG CAGCTATGGG 
ACGTCTACCC CATATTCCAC TCCTCGGCTA ACACTATCAC AACTGCCAGG GCAGCTACCT 
CAGATTCTGA GCCCGCAGTC AATAAAGCTG TCAACTGAGC CACAGCAGGT TACCATCTGG 
AGTCCTTCTG CAGTTTGTGG TATGACCACT CCACCAACCT CCCCTGGAAT TGTCCCATCA 
GAGTCATCCC AGTCAACATC ACAACCTTAC AGCAAAGCTT TTGGCACATC TGCAGTGGGG 
AAGGGAACAC CTTTGGGGAC ACCAGCCACA TCTCCTCCAC CCTGCACTTC AGATGACTTT 
GTGCATGTCT CACTTCCTTC AGCTGCTGCC ACACCTCCTA AAAAGGAGGA CAAACCAGAT 
TCTGGGAGGC CTTCACTGTA CCGACAGCAA AATGTCATAA GCAGCGATAA ATCATTGGAC 
ACATCTGGTA GTAAAAGTTC AGTAACCTTA AGTGATCTTC CGGAGTTTTT AGGCGGTTTG 
TCTTTTGAAG ATAGTGCTGA AAAGGACAGA GAAGAAGATG CAATATCTAA AGAGATCTCT 
GAGATCACGA CTGATGCTGA ACACATGGTG CCTAGAGGAG GATTTGACTC TCCATTTTAT 
CACACAAATG AAAATCTGTC AGGTTCTCAG AAGAAGACCC AGTCAGTAGT CTCTAGTGTG 
CAGGGACACA GTCTGACCTC TGAGCCTTTA ACGTCTTCTC TGGACAAGCC TGGGCCTGAG 
AGTGCACTGG AGACACCCAA ACAAACATTT ACTCCCATAG ACAAGCCCTG CGGAGGCTCT 
GGTGAAAGCC CTGCTGGTAA CAGGGAAGGA ACCTCTGGGG AGACAAGTAT TCTCACTCCC 
AGCCCTTGCA AAGCATCAGC ACAAGGAAGA GCAGTGTTTG GGAGTGGGCA GCCTCCCCTG 
TATGAGCACC TTTTTGAGGT TGCGCTACCA AAGACTGCCT ACCTCTTTGT TGGCAAGAAG 
ACTGAGGAGC TGCTAAAGAA AGCCAAGGGA ACCCAGGATA AAGACTGCAT GTCCTCTACT 
TCTCCTGTGG AAGTACTGGA CAGACTGATA CAGCAAGGGG CAGATGCACA CACTAAGGAA 
CTGAACAAAT TGTCTCTGCC AAGCAAATCT GCTGACTGGA CTCACTTTGG AGGTTCTCCC 
CCTTCAGATG AGATTCACAC CCTGCGTAAC CAATTGCTGT TGCTGCACAA CCAGTTGCTG 
TATGAACGCT TCAAAAGACA GCAGCATGCC CTTCGGAACC GGCGGCTCTT GCGGAAAGTG 
ATTAAAGCCA CAGCACTGGA GGAACATAAT GCTGCCATGA AAGATCAGCT GAAATTACAG 
GAAAAAGAAA TCCAGGCCTT GAAACTGAGT CTGCAGAAAG AACAGGCCAG GTACCACCAG 
TTTCAGGAGG AGCACGAAGG GATAGTGGCT CAGCTTCACA GCCAGATCAG ACAGCTGCAA 
CATGACCGTG AGGAATTCTA CAACCAGAGC CAGGAATTGC AGACCAAGCT GGAAGACTGC 
AGGAACATGA TTGCAGATCT GAGGTTAGAA TTGAAAAAGG CTAACAACAA AGTCTGTCAC 
ACAGAACTGC TTCTTAGCCA AGTTTCTCAA AAGCTTTCCA ACAGTGAATC AGTGCAACAG 
CAGATGGAGT TCTTGAACAG ACAACTTCTG GTTCTTGGAG AGGTCAATGA GTTGTACCTG 
GAGCAGCTGC AGCACAAGCA CACAGACACT ACAAAGGAGG TTGAAATGTT GCATGCTGCT 
TTTCGAAAGG AACTGGAGAA AGCAAAATTG TGTGTTCAGC AGCAAAGCCA AAGGCTCGAT 
GCTTCTCAGA AACGGATAGC TGAACTGGAA TCTCAGCTTT CTAAAAAGGA CCACCTCTTC 
TTGGAGCAAA AGAAATACCT GGAAGATGTC AAAATCAAAG CAAGAGGTCA GTTGCAAGCA 
GTAGAGAGTA GATACAGGGC CCAGAAAAGG ATCACCCAGG CGTTTGAGCT GGAGATCTTG 
AACCTGTTTG GCCGACTGGA GAACAGCCCG CTGAAAAAAC TTGAAGATGA AAAAACAGAA 
GCAGCTGAAG CAGCAGAAGA AAGGCTGGAT TGTGGTAATG AAGGTTGTGC AGATACTGTG 
GCAGGATACA GCGAAGAAAC CATTGATAGA AATGGAGAAA CCAAACCTCC CAGCACTCGA 
GGTAGCAGTA AAGGTGGCAG CAACAGTGAG CTCTCCACCC CAGAAAAAAA ACAGAACCAG 
AGATTCAGCA GTCACTGGGA GACCTCCATG TTGCGGGAGC CCTCCACTAC TGTCCCACTG 
ACTGTAGGTT CACTCCCCAG CTCCAAGAGC TTCCTTGGTA TGAAGTCACG AGAGTTGTTT 
CGCAACAAAA GTGAGAGCCA GTGTGATGAG CATGGTGTAA CCATCAACAG CCTTTCTGAT 
ACTCTAAAGA CTGAACTATG TAAAGATCCA AGCATGGAGA CGAAGGCTCC TCCAAGCCCC 
GATAACCTTA GCCTCTCACC TAAGATCCAG GAAAGCAGTG TTGGACAGCT TCATATCATG 
GACTACAATG AAACTCATCA TGACCACAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135247.1
CDS	77..3049
Translation	MSQGHVAEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPAEASYEDGYYSASRKSCTNLKHHQTDPSASHYIDTQSSYGTSTPYSTPRLTLSQLPGQLPQILSPQSIKLSTEPQQVTIWSPSAVCGMTTPPTSPGIVPSESSQSTSQPYSKAFGTSAVGKGTPLGTPATSPPPCTSDDFVHVSLPSAAATPPKKEDKPDSGRPSLYRQQNVISSDKSLDTSGSKSSVTLSDLPEFLGGLSFEDSAEKDREEDAISKEISEITTDAEHMVPRGGFDSPFYHTNENLSGSQKKTQSVVSSVQGHSLTSEPLTSSLDKPGPESALETPKQTFTPIDKPCGGSGESPAGNREGTSGETSILTPSPCKASAQGRAVFGSGQPPLYEHLFEVALPKTAYLFVGKKTEELLKKAKGTQDKDCMSSTSPVEVLDRLIQQGADAHTKELNKLSLPSKSADWTHFGGSPPSDEIHTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKATALEEHNAAMKDQLKLQEKEIQALKLSLQKEQARYHQFQEEHEGIVAQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIADLRLELKKANNKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQHKHTDTTKEVEMLHAAFRKELEKAKLCVQQQSQRLDASQKRIAELESQLSKKDHLFLEQKKYLEDVKIKARGQLQAVESRYRAQKRITQAFELEILNLFGRLENSPLKKLEDEKTEAAEAAEERLDCGNEGCADTVAGYSEETIDRNGETKPPSTRGSSKGGSNSELSTPEKKQNQRFSSHWETSMLREPSTTVPLTVGSLPSSKSFLGMKSRELFRNKSESQCDEHGVTINSLSDTLKTELCKDPSMETKAPPSPDNLSLSPKIQESSVGQLHIMDYNETHHDHS

Target ORF information:

RefSeq Version	XM_015279761.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279761.1

ORF Insert Sequence:

ATGTCTCAAG GTCACGTGGC AGAGATTTAC CTTGTCCATC TTCATGCCAG TGTTTATGCT 
CTCTTTCATC GGCTTTATGG AATGTATCCT TGCAATTTTG TCTCCTTTCT GCGTTCTCAC 
TACAGTATGA AGGAAAACTT GGAGACCTTT GAAGAGGTAG TCAAGCCAAT GATGGAACAT 
GTGCGAATTC ATCCAGAATT AGTGACTGGA TCTAAGGACC ATGAACTGGA CCCACGAAGG 
TGGAAAAGAC TAGAAACTCA TGATGTTGTG ATAGAATGTG CCAAAATCTC CCTGGACCCT 
GCAGAAGCCT CATATGAAGA TGGCTATTAC TCTGCGTCTC GGAAATCCTG TACAAACTTA 
AAACATCATC AAACTGACCC CAGTGCCAGC CATTACATTG ACACACAGAG CAGCTATGGG 
ACGTCTACCC CATATTCCAC TCCTCGGCTA ACACTATCAC AACTGCCAGG GCAGCTACCT 
CAGATTCTGA GCCCGCAGTC AATAAAGCTG TCAACTGAGC CACAGCAGGT TACCATCTGG 
AGTCCTTCTG CAGTTTGTGG TATGACCACT CCACCAACCT CCCCTGGAAT TGTCCCATCA 
GAGTCATCCC AGTCAACATC ACAACCTTAC AGCAAAGCTT TTGGCACATC TGCAGTGGGG 
AAGGGAACAC CTTTGGGGAC ACCAGCCACA TCTCCTCCAC CCTGCACTTC AGATGACTTT 
GTGCATGTCT CACTTCCTTC AGCTGCTGCC ACACCTCCTA AAAAGGAGGA CAAACCAGAT 
TCTGGGAGGC CTTCACTGTA CCGACAGCAA AATGTCATAA GCAGCGATAA ATCATTGGAC 
ACATCTGGTA GTAAAAGTTC AGTAACCTTA AGTGATCTTC CGGAGTTTTT AGGCGGTTTG 
TCTTTTGAAG ATAGTGCTGA AAAGGACAGA GAAGAAGATG CAATATCTAA AGAGATCTCT 
GAGATCACGA CTGATGCTGA ACACATGGTG CCTAGAGGAG GATTTGACTC TCCATTTTAT 
CACACAAATG AAAATCTGTC AGGTTCTCAG AAGAAGACCC AGTCAGTAGT CTCTAGTGTG 
CAGGGACACA GTCTGACCTC TGAGCCTTTA ACGTCTTCTC TGGACAAGCC TGGGCCTGAG 
AGTGCACTGG AGACACCCAA ACAAACATTT ACTCCCATAG ACAAGCCCTG CGGAGGCTCT 
GGTGAAAGCC CTGCTGGTAA CAGGGAAGGA ACCTCTGGGG AGACAAGTAT TCTCACTCCC 
AGCCCTTGCA AAGCATCAGC ACAAGGAAGA GCAGTGTTTG GGAGTGGGCA GCCTCCCCTG 
TATGAGCACC TTTTTGAGGT TGCGCTACCA AAGACTGCCT ACCTCTTTGT TGGCAAGAAG 
ACTGAGGAGC TGCTAAAGAA AGCCAAGGGA ACCCAGGATA AAGACTGCAT GTCCTCTACT 
TCTCCTGTGG AAGTACTGGA CAGACTGATA CAGCAAGGGG CAGATGCACA CACTAAGGAA 
CTGAACAAAT TGTCTCTGCC AAGCAAATCT GCTGACTGGA CTCACTTTGG AGGTTCTCCC 
CCTTCAGATG AGATTCACAC CCTGCGTAAC CAATTGCTGT TGCTGCACAA CCAGTTGCTG 
TATGAACGCT TCAAAAGACA GCAGCATGCC CTTCGGAACC GGCGGCTCTT GCGGAAAGTG 
ATTAAAGCCA CAGCACTGGA GGAACATAAT GCTGCCATGA AAGATCAGCT GAAATTACAG 
GAAAAAGAAA TCCAGGCCTT GAAACTGAGT CTGCAGAAAG AACAGGCCAG GTACCACCAG 
TTTCAGGAGG AGCACGAAGG GATAGTGGCT CAGCTTCACA GCCAGATCAG ACAGCTGCAA 
CATGACCGTG AGGAATTCTA CAACCAGAGC CAGGAATTGC AGACCAAGCT GGAAGACTGC 
AGGAACATGA TTGCAGATCT GAGGTTAGAA TTGAAAAAGG CTAACAACAA AGTCTGTCAC 
ACAGAACTGC TTCTTAGCCA AGTTTCTCAA AAGCTTTCCA ACAGTGAATC AGTGCAACAG 
CAGATGGAGT TCTTGAACAG ACAACTTCTG GTTCTTGGAG AGGTCAATGA GTTGTACCTG 
GAGCAGCTGC AGCACAAGCA CACAGACACT ACAAAGGAGG TTGAAATGTT GCATGCTGCT 
TTTCGAAAGG AACTGGAGAA AGCAAAATTG TGTGTTCAGC AGCAAAGCCA AAGGCTCGAT 
GCTTCTCAGA AACGGATAGC TGAACTGGAA TCTCAGCTTT CTAAAAAGGA CCACCTCTTC 
TTGGAGCAAA AGAAATACCT GGAAGATGTC AAAATCAAAG CAAGAGGTCA GTTGCAAGCA 
GTAGAGAGTA GATACAGGGC CCAGAAAAGG ATCACCCAGG CGTTTGAGCT GGAGATCTTG 
AACCTGTTTG GCCGACTGGA GAACAGCCCG CTGAAAAAAC TTGAAGATGA AAAAACAGAA 
GCAGCTGAAG CAGCAGAAGA AAGGCTGGAT TGTGGTAATG AAGGTTGTGC AGATACTGTG 
GCAGGATACA GCGAAGAAAC CATTGATAGA AATGGAGAAA CCAAACCTCC CAGCACTCGA 
GGTAGCAGTA AAGGTGGCAG CAACAGTGAG CTCTCCACCC CAGAAAAAAA ACAGAACCAG 
AGATTCAGCA GTCACTGGGA GACCTCCATG TTGCGGGAGC CCTCCACTAC TGTCCCACTG 
ACTGTAGGTT CACTCCCCAG CTCCAAGAGC TTCCTTGGTA TGAAGTCACG AGAGTTGTTT 
CGCAACAAAA GTGAGAGCCA GTGTGATGAG CATGGTGTAA CCATCAACAG CCTTTCTGAT 
ACTCTAAAGA CTGAACTATG TAAAGATCCA AGCATGGAGA CGAAGGCTCC TCCAAGCCCC 
GATAACCTTA GCCTCTCACC TAAGATCCAG GAAAGCAGTG TTGGACAGCT TCATATCATG 
GACTACAATG AAACTCATCA TGACCACAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_015279761.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135247.1 Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OGa43675
Clone ID Related Accession (Same CDS sequence)	XM_015279761.1 , XM_015279761.2
Accession Version	XM_015279761.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	hamartin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279761.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAAG GTCACGTGGC AGAGATTTAC CTTGTCCATC TTCATGCCAG TGTTTATGCT 
CTCTTTCATC GGCTTTATGG AATGTATCCT TGCAATTTTG TCTCCTTTCT GCGTTCTCAC 
TACAGTATGA AGGAAAACTT GGAGACCTTT GAAGAGGTAG TCAAGCCAAT GATGGAACAT 
GTGCGAATTC ATCCAGAATT AGTGACTGGA TCTAAGGACC ATGAACTGGA CCCACGAAGG 
TGGAAAAGAC TAGAAACTCA TGATGTTGTG ATAGAATGTG CCAAAATCTC CCTGGACCCT 
GCAGAAGCCT CATATGAAGA TGGCTATTAC TCTGCGTCTC GGAAATCCTG TACAAACTTA 
AAACATCATC AAACTGACCC CAGTGCCAGC CATTACATTG ACACACAGAG CAGCTATGGG 
ACGTCTACCC CATATTCCAC TCCTCGGCTA ACACTATCAC AACTGCCAGG GCAGCTACCT 
CAGATTCTGA GCCCGCAGTC AATAAAGCTG TCAACTGAGC CACAGCAGGT TACCATCTGG 
AGTCCTTCTG CAGTTTGTGG TATGACCACT CCACCAACCT CCCCTGGAAT TGTCCCATCA 
GAGTCATCCC AGTCAACATC ACAACCTTAC AGCAAAGCTT TTGGCACATC TGCAGTGGGG 
AAGGGAACAC CTTTGGGGAC ACCAGCCACA TCTCCTCCAC CCTGCACTTC AGATGACTTT 
GTGCATGTCT CACTTCCTTC AGCTGCTGCC ACACCTCCTA AAAAGGAGGA CAAACCAGAT 
TCTGGGAGGC CTTCACTGTA CCGACAGCAA AATGTCATAA GCAGCGATAA ATCATTGGAC 
ACATCTGGTA GTAAAAGTTC AGTAACCTTA AGTGATCTTC CGGAGTTTTT AGGCGGTTTG 
TCTTTTGAAG ATAGTGCTGA AAAGGACAGA GAAGAAGATG CAATATCTAA AGAGATCTCT 
GAGATCACGA CTGATGCTGA ACACATGGTG CCTAGAGGAG GATTTGACTC TCCATTTTAT 
CACACAAATG AAAATCTGTC AGGTTCTCAG AAGAAGACCC AGTCAGTAGT CTCTAGTGTG 
CAGGGACACA GTCTGACCTC TGAGCCTTTA ACGTCTTCTC TGGACAAGCC TGGGCCTGAG 
AGTGCACTGG AGACACCCAA ACAAACATTT ACTCCCATAG ACAAGCCCTG CGGAGGCTCT 
GGTGAAAGCC CTGCTGGTAA CAGGGAAGGA ACCTCTGGGG AGACAAGTAT TCTCACTCCC 
AGCCCTTGCA AAGCATCAGC ACAAGGAAGA GCAGTGTTTG GGAGTGGGCA GCCTCCCCTG 
TATGAGCACC TTTTTGAGGT TGCGCTACCA AAGACTGCCT ACCTCTTTGT TGGCAAGAAG 
ACTGAGGAGC TGCTAAAGAA AGCCAAGGGA ACCCAGGATA AAGACTGCAT GTCCTCTACT 
TCTCCTGTGG AAGTACTGGA CAGACTGATA CAGCAAGGGG CAGATGCACA CACTAAGGAA 
CTGAACAAAT TGTCTCTGCC AAGCAAATCT GCTGACTGGA CTCACTTTGG AGGTTCTCCC 
CCTTCAGATG AGATTCACAC CCTGCGTAAC CAATTGCTGT TGCTGCACAA CCAGTTGCTG 
TATGAACGCT TCAAAAGACA GCAGCATGCC CTTCGGAACC GGCGGCTCTT GCGGAAAGTG 
ATTAAAGCCA CAGCACTGGA GGAACATAAT GCTGCCATGA AAGATCAGCT GAAATTACAG 
GAAAAAGAAA TCCAGGCCTT GAAACTGAGT CTGCAGAAAG AACAGGCCAG GTACCACCAG 
TTTCAGGAGG AGCACGAAGG GATAGTGGCT CAGCTTCACA GCCAGATCAG ACAGCTGCAA 
CATGACCGTG AGGAATTCTA CAACCAGAGC CAGGAATTGC AGACCAAGCT GGAAGACTGC 
AGGAACATGA TTGCAGATCT GAGGTTAGAA TTGAAAAAGG CTAACAACAA AGTCTGTCAC 
ACAGAACTGC TTCTTAGCCA AGTTTCTCAA AAGCTTTCCA ACAGTGAATC AGTGCAACAG 
CAGATGGAGT TCTTGAACAG ACAACTTCTG GTTCTTGGAG AGGTCAATGA GTTGTACCTG 
GAGCAGCTGC AGCACAAGCA CACAGACACT ACAAAGGAGG TTGAAATGTT GCATGCTGCT 
TTTCGAAAGG AACTGGAGAA AGCAAAATTG TGTGTTCAGC AGCAAAGCCA AAGGCTCGAT 
GCTTCTCAGA AACGGATAGC TGAACTGGAA TCTCAGCTTT CTAAAAAGGA CCACCTCTTC 
TTGGAGCAAA AGAAATACCT GGAAGATGTC AAAATCAAAG CAAGAGGTCA GTTGCAAGCA 
GTAGAGAGTA GATACAGGGC CCAGAAAAGG ATCACCCAGG CGTTTGAGCT GGAGATCTTG 
AACCTGTTTG GCCGACTGGA GAACAGCCCG CTGAAAAAAC TTGAAGATGA AAAAACAGAA 
GCAGCTGAAG CAGCAGAAGA AAGGCTGGAT TGTGGTAATG AAGGTTGTGC AGATACTGTG 
GCAGGATACA GCGAAGAAAC CATTGATAGA AATGGAGAAA CCAAACCTCC CAGCACTCGA 
GGTAGCAGTA AAGGTGGCAG CAACAGTGAG CTCTCCACCC CAGAAAAAAA ACAGAACCAG 
AGATTCAGCA GTCACTGGGA GACCTCCATG TTGCGGGAGC CCTCCACTAC TGTCCCACTG 
ACTGTAGGTT CACTCCCCAG CTCCAAGAGC TTCCTTGGTA TGAAGTCACG AGAGTTGTTT 
CGCAACAAAA GTGAGAGCCA GTGTGATGAG CATGGTGTAA CCATCAACAG CCTTTCTGAT 
ACTCTAAAGA CTGAACTATG TAAAGATCCA AGCATGGAGA CGAAGGCTCC TCCAAGCCCC 
GATAACCTTA GCCTCTCACC TAAGATCCAG GAAAGCAGTG TTGGACAGCT TCATATCATG 
GACTACAATG AAACTCATCA TGACCACAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135247.1
CDS	129..3101
Translation	MSQGHVAEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPAEASYEDGYYSASRKSCTNLKHHQTDPSASHYIDTQSSYGTSTPYSTPRLTLSQLPGQLPQILSPQSIKLSTEPQQVTIWSPSAVCGMTTPPTSPGIVPSESSQSTSQPYSKAFGTSAVGKGTPLGTPATSPPPCTSDDFVHVSLPSAAATPPKKEDKPDSGRPSLYRQQNVISSDKSLDTSGSKSSVTLSDLPEFLGGLSFEDSAEKDREEDAISKEISEITTDAEHMVPRGGFDSPFYHTNENLSGSQKKTQSVVSSVQGHSLTSEPLTSSLDKPGPESALETPKQTFTPIDKPCGGSGESPAGNREGTSGETSILTPSPCKASAQGRAVFGSGQPPLYEHLFEVALPKTAYLFVGKKTEELLKKAKGTQDKDCMSSTSPVEVLDRLIQQGADAHTKELNKLSLPSKSADWTHFGGSPPSDEIHTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKATALEEHNAAMKDQLKLQEKEIQALKLSLQKEQARYHQFQEEHEGIVAQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIADLRLELKKANNKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQHKHTDTTKEVEMLHAAFRKELEKAKLCVQQQSQRLDASQKRIAELESQLSKKDHLFLEQKKYLEDVKIKARGQLQAVESRYRAQKRITQAFELEILNLFGRLENSPLKKLEDEKTEAAEAAEERLDCGNEGCADTVAGYSEETIDRNGETKPPSTRGSSKGGSNSELSTPEKKQNQRFSSHWETSMLREPSTTVPLTVGSLPSSKSFLGMKSRELFRNKSESQCDEHGVTINSLSDTLKTELCKDPSMETKAPPSPDNLSLSPKIQESSVGQLHIMDYNETHHDHS

Target ORF information:

RefSeq Version	XM_015279761.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279761.2

ORF Insert Sequence:

ATGTCTCAAG GTCACGTGGC AGAGATTTAC CTTGTCCATC TTCATGCCAG TGTTTATGCT 
CTCTTTCATC GGCTTTATGG AATGTATCCT TGCAATTTTG TCTCCTTTCT GCGTTCTCAC 
TACAGTATGA AGGAAAACTT GGAGACCTTT GAAGAGGTAG TCAAGCCAAT GATGGAACAT 
GTGCGAATTC ATCCAGAATT AGTGACTGGA TCTAAGGACC ATGAACTGGA CCCACGAAGG 
TGGAAAAGAC TAGAAACTCA TGATGTTGTG ATAGAATGTG CCAAAATCTC CCTGGACCCT 
GCAGAAGCCT CATATGAAGA TGGCTATTAC TCTGCGTCTC GGAAATCCTG TACAAACTTA 
AAACATCATC AAACTGACCC CAGTGCCAGC CATTACATTG ACACACAGAG CAGCTATGGG 
ACGTCTACCC CATATTCCAC TCCTCGGCTA ACACTATCAC AACTGCCAGG GCAGCTACCT 
CAGATTCTGA GCCCGCAGTC AATAAAGCTG TCAACTGAGC CACAGCAGGT TACCATCTGG 
AGTCCTTCTG CAGTTTGTGG TATGACCACT CCACCAACCT CCCCTGGAAT TGTCCCATCA 
GAGTCATCCC AGTCAACATC ACAACCTTAC AGCAAAGCTT TTGGCACATC TGCAGTGGGG 
AAGGGAACAC CTTTGGGGAC ACCAGCCACA TCTCCTCCAC CCTGCACTTC AGATGACTTT 
GTGCATGTCT CACTTCCTTC AGCTGCTGCC ACACCTCCTA AAAAGGAGGA CAAACCAGAT 
TCTGGGAGGC CTTCACTGTA CCGACAGCAA AATGTCATAA GCAGCGATAA ATCATTGGAC 
ACATCTGGTA GTAAAAGTTC AGTAACCTTA AGTGATCTTC CGGAGTTTTT AGGCGGTTTG 
TCTTTTGAAG ATAGTGCTGA AAAGGACAGA GAAGAAGATG CAATATCTAA AGAGATCTCT 
GAGATCACGA CTGATGCTGA ACACATGGTG CCTAGAGGAG GATTTGACTC TCCATTTTAT 
CACACAAATG AAAATCTGTC AGGTTCTCAG AAGAAGACCC AGTCAGTAGT CTCTAGTGTG 
CAGGGACACA GTCTGACCTC TGAGCCTTTA ACGTCTTCTC TGGACAAGCC TGGGCCTGAG 
AGTGCACTGG AGACACCCAA ACAAACATTT ACTCCCATAG ACAAGCCCTG CGGAGGCTCT 
GGTGAAAGCC CTGCTGGTAA CAGGGAAGGA ACCTCTGGGG AGACAAGTAT TCTCACTCCC 
AGCCCTTGCA AAGCATCAGC ACAAGGAAGA GCAGTGTTTG GGAGTGGGCA GCCTCCCCTG 
TATGAGCACC TTTTTGAGGT TGCGCTACCA AAGACTGCCT ACCTCTTTGT TGGCAAGAAG 
ACTGAGGAGC TGCTAAAGAA AGCCAAGGGA ACCCAGGATA AAGACTGCAT GTCCTCTACT 
TCTCCTGTGG AAGTACTGGA CAGACTGATA CAGCAAGGGG CAGATGCACA CACTAAGGAA 
CTGAACAAAT TGTCTCTGCC AAGCAAATCT GCTGACTGGA CTCACTTTGG AGGTTCTCCC 
CCTTCAGATG AGATTCACAC CCTGCGTAAC CAATTGCTGT TGCTGCACAA CCAGTTGCTG 
TATGAACGCT TCAAAAGACA GCAGCATGCC CTTCGGAACC GGCGGCTCTT GCGGAAAGTG 
ATTAAAGCCA CAGCACTGGA GGAACATAAT GCTGCCATGA AAGATCAGCT GAAATTACAG 
GAAAAAGAAA TCCAGGCCTT GAAACTGAGT CTGCAGAAAG AACAGGCCAG GTACCACCAG 
TTTCAGGAGG AGCACGAAGG GATAGTGGCT CAGCTTCACA GCCAGATCAG ACAGCTGCAA 
CATGACCGTG AGGAATTCTA CAACCAGAGC CAGGAATTGC AGACCAAGCT GGAAGACTGC 
AGGAACATGA TTGCAGATCT GAGGTTAGAA TTGAAAAAGG CTAACAACAA AGTCTGTCAC 
ACAGAACTGC TTCTTAGCCA AGTTTCTCAA AAGCTTTCCA ACAGTGAATC AGTGCAACAG 
CAGATGGAGT TCTTGAACAG ACAACTTCTG GTTCTTGGAG AGGTCAATGA GTTGTACCTG 
GAGCAGCTGC AGCACAAGCA CACAGACACT ACAAAGGAGG TTGAAATGTT GCATGCTGCT 
TTTCGAAAGG AACTGGAGAA AGCAAAATTG TGTGTTCAGC AGCAAAGCCA AAGGCTCGAT 
GCTTCTCAGA AACGGATAGC TGAACTGGAA TCTCAGCTTT CTAAAAAGGA CCACCTCTTC 
TTGGAGCAAA AGAAATACCT GGAAGATGTC AAAATCAAAG CAAGAGGTCA GTTGCAAGCA 
GTAGAGAGTA GATACAGGGC CCAGAAAAGG ATCACCCAGG CGTTTGAGCT GGAGATCTTG 
AACCTGTTTG GCCGACTGGA GAACAGCCCG CTGAAAAAAC TTGAAGATGA AAAAACAGAA 
GCAGCTGAAG CAGCAGAAGA AAGGCTGGAT TGTGGTAATG AAGGTTGTGC AGATACTGTG 
GCAGGATACA GCGAAGAAAC CATTGATAGA AATGGAGAAA CCAAACCTCC CAGCACTCGA 
GGTAGCAGTA AAGGTGGCAG CAACAGTGAG CTCTCCACCC CAGAAAAAAA ACAGAACCAG 
AGATTCAGCA GTCACTGGGA GACCTCCATG TTGCGGGAGC CCTCCACTAC TGTCCCACTG 
ACTGTAGGTT CACTCCCCAG CTCCAAGAGC TTCCTTGGTA TGAAGTCACG AGAGTTGTTT 
CGCAACAAAA GTGAGAGCCA GTGTGATGAG CATGGTGTAA CCATCAACAG CCTTTCTGAT 
ACTCTAAAGA CTGAACTATG TAAAGATCCA AGCATGGAGA CGAAGGCTCC TCCAAGCCCC 
GATAACCTTA GCCTCTCACC TAAGATCCAG GAAAGCAGTG TTGGACAGCT TCATATCATG 
GACTACAATG AAACTCATCA TGACCACAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_015279760.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135246.1 Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X2, mRNA.

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CloneID	OGa43672
Clone ID Related Accession (Same CDS sequence)	XM_015279760.1 , XM_415449.6 , XM_415449.5 , XM_015279760.2
Accession Version	XM_015279760.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGC AGGGGAATGT TGGTGAGCTC CTCTCAATGC TGGATTCTCC AATTCTGGGT 
GTCCTGGAAG ACATAACAGC TGCATTCAAA GATAATCTCA TTTGTGACCG TGGACCTATG 
CTTGTGAACA GCCTGGTAGA CTATTACTTG GAAACCAATT CTCAGCAGGC ACTGCATATA 
CTGTCCACAT TGCAGGAGCC TCATGACAAG CACCTTCTGG ACAAAATTAA TGAATATATG 
GGCAAAGCTG CTACTCGTTT ACCCACTCTC TCGCTGCTGG GACATGTGAT AAGGCGACAA 
CCATCGTGGA AACATAAGCT TTCTCAAGCA CCTCTCCTAC TTTCATTACT TAAATGTCTC 
AAGACTGACA CAGATGTAGT AGTACTCACC ACAGGTGTCC TAGTGCTAAT AACCATGTTG 
CCAATGATTC CTCAGTCTGG CAAACAGTAC CTCCATGATT TCTTTGGTAT CTTTGGGCGT 
CTCTCAGCCT GGTGCTTGCA GAATCCAGGT CACGTGGCAG AGATTTACCT TGTCCATCTT 
CATGCCAGTG TTTATGCTCT CTTTCATCGG CTTTATGGAA TGTATCCTTG CAATTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACTTGG AGACCTTTGA AGAGGTAGTC 
AAGCCAATGA TGGAACATGT GCGAATTCAT CCAGAATTAG TGACTGGATC TAAGGACCAT 
GAACTGGACC CACGAAGGTG GAAAAGACTA GAAACTCATG ATGTTGTGAT AGAATGTGCC 
AAAATCTCCC TGGACCCTGC AGAAGCCTCA TATGAAGATG GCTATTACTC TGCGTCTCGG 
AAATCCTGTA CAAACTTAAA ACATCATCAA ACTGACCCCA GTGCCAGCCA TTACATTGAC 
ACACAGAGCA GCTATGGGAC GTCTACCCCA TATTCCACTC CTCGGCTAAC ACTATCACAA 
CTGCCAGGGC AGCTACCTCA GATTCTGAGC CCGCAGTCAA TAAAGCTGTC AACTGAGCCA 
CAGCAGGTTA CCATCTGGAG TCCTTCTGCA GTTTGTGGTA TGACCACTCC ACCAACCTCC 
CCTGGAATTG TCCCATCAGA GTCATCCCAG TCAACATCAC AACCTTACAG CAAAGCTTTT 
GGCACATCTG CAGTGGGGAA GGGAACACCT TTGGGGACAC CAGCCACATC TCCTCCACCC 
TGCACTTCAG ATGACTTTGT GCATGTCTCA CTTCCTTCAG CTGCTGCCAC ACCTCCTAAA 
AAGGAGGACA AACCAGATTC TGGGAGGCCT TCACTGTACC GACAGCAAAA TGTCATAAGC 
AGCGATAAAT CATTGGACAC ATCTGGTAGT AAAAGTTCAG TAACCTTAAG TGATCTTCCG 
GAGTTTTTAG GCGGTTTGTC TTTTGAAGAT AGTGCTGAAA AGGACAGAGA AGAAGATGCA 
ATATCTAAAG AGATCTCTGA GATCACGACT GATGCTGAAC ACATGGTGCC TAGAGGAGGA 
TTTGACTCTC CATTTTATCA CACAAATGAA AATCTGTCAG GTTCTCAGAA GAAGACCCAG 
TCAGTAGTCT CTAGTGTGCA GGGACACAGT CTGACCTCTG AGCCTTTAAC GTCTTCTCTG 
GACAAGCCTG GGCCTGAGAG TGCACTGGAG ACACCCAAAC AAACATTTAC TCCCATAGAC 
AAGCCCTGCG GAGGCTCTGG TGAAAGCCCT GCTGGTAACA GGGAAGGAAC CTCTGGGGAG 
ACAAGTATTC TCACTCCCAG CCCTTGCAAA GCATCAGCAC AAGGAAGAGC AGTGTTTGGG 
AGTGGGCAGC CTCCCCTGTA TGAGCACCTT TTTGAGGTTG CGCTACCAAA GACTGCCTAC 
CTCTTTGTTG GCAAGAAGAC TGAGGAGCTG CTAAAGAAAG CCAAGGGAAC CCAGGATAAA 
GACTGCATGT CCTCTACTTC TCCTGTGGAA GTACTGGACA GACTGATACA GCAAGGGGCA 
GATGCACACA CTAAGGAACT GAACAAATTG TCTCTGCCAA GCAAATCTGC TGACTGGACT 
CACTTTGGAG GTTCTCCCCC TTCAGATGAG ATTCACACCC TGCGTAACCA ATTGCTGTTG 
CTGCACAACC AGTTGCTGTA TGAACGCTTC AAAAGACAGC AGCATGCCCT TCGGAACCGG 
CGGCTCTTGC GGAAAGTGAT TAAAGCCACA GCACTGGAGG AACATAATGC TGCCATGAAA 
GATCAGCTGA AATTACAGGA AAAAGAAATC CAGGCCTTGA AACTGAGTCT GCAGAAAGAA 
CAGGCCAGGT ACCACCAGTT TCAGGAGGAG CACGAAGGGA TAGTGGCTCA GCTTCACAGC 
CAGATCAGAC AGCTGCAACA TGACCGTGAG GAATTCTACA ACCAGAGCCA GGAATTGCAG 
ACCAAGCTGG AAGACTGCAG GAACATGATT GCAGATCTGA GGTTAGAATT GAAAAAGGCT 
AACAACAAAG TCTGTCACAC AGAACTGCTT CTTAGCCAAG TTTCTCAAAA GCTTTCCAAC 
AGTGAATCAG TGCAACAGCA GATGGAGTTC TTGAACAGAC AACTTCTGGT TCTTGGAGAG 
GTCAATGAGT TGTACCTGGA GCAGCTGCAG CACAAGCACA CAGACACTAC AAAGGAGGTT 
GAAATGTTGC ATGCTGCTTT TCGAAAGGAA CTGGAGAAAG CAAAATTGTG TGTTCAGCAG 
CAAAGCCAAA GGCTCGATGC TTCTCAGAAA CGGATAGCTG AACTGGAATC TCAGCTTTCT 
AAAAAGGACC ACCTCTTCTT GGAGCAAAAG AAATACCTGG AAGATGTCAA AATCAAAGCA 
AGAGGTCAGT TGCAAGCAGT AGAGAGTAGA TACAGGGCCC AGAAAAGGAT CACCCAGGCG 
TTTGAGCTGG AGATCTTGAA CCTGTTTGGC CGACTGGAGA ACAGCCCGCT GAAAAAACTT 
GAAGATGAAA AAACAGAAGC AGCTGAAGCA GCAGAAGAAA GGCTGGATTG TGGTAATGAA 
GGTTGTGCAG ATACTGTGGC AGGATACAGC GAAGAAACCA TTGATAGAAA TGGAGAAACC 
AAACCTCCCA GCACTCGAGG TAGCAGTAAA GGTGGCAGCA ACAGTGAGCT CTCCACCCCA 
GAAAAAAAAC AGAACCAGAG ATTCAGCAGT CACTGGGAGA CCTCCATGTT GCGGGAGCCC 
TCCACTACTG TCCCACTGAC TGTAGGTTCA CTCCCCAGCT CCAAGAGCTT CCTTGGTATG 
AAGTCACGAG AGTTGTTTCG CAACAAAAGT GAGAGCCAGT GTGATGAGCA TGGTGTAACC 
ATCAACAGCC TTTCTGATAC TCTAAAGACT GAACTATGTA AAGATCCAAG CATGGAGACG 
AAGGCTCCTC CAAGCCCCGA TAACCTTAGC CTCTCACCTA AGATCCAGGA AAGCAGTGTT 
GGACAGCTTC ATATCATGGA CTACAATGAA ACTCATCATG ACCACAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135246.1
CDS	176..3646
Translation	MAQQGNVGELLSMLDSPILGVLEDITAAFKDNLICDRGPMLVNSLVDYYLETNSQQALHILSTLQEPHDKHLLDKINEYMGKAATRLPTLSLLGHVIRRQPSWKHKLSQAPLLLSLLKCLKTDTDVVVLTTGVLVLITMLPMIPQSGKQYLHDFFGIFGRLSAWCLQNPGHVAEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPAEASYEDGYYSASRKSCTNLKHHQTDPSASHYIDTQSSYGTSTPYSTPRLTLSQLPGQLPQILSPQSIKLSTEPQQVTIWSPSAVCGMTTPPTSPGIVPSESSQSTSQPYSKAFGTSAVGKGTPLGTPATSPPPCTSDDFVHVSLPSAAATPPKKEDKPDSGRPSLYRQQNVISSDKSLDTSGSKSSVTLSDLPEFLGGLSFEDSAEKDREEDAISKEISEITTDAEHMVPRGGFDSPFYHTNENLSGSQKKTQSVVSSVQGHSLTSEPLTSSLDKPGPESALETPKQTFTPIDKPCGGSGESPAGNREGTSGETSILTPSPCKASAQGRAVFGSGQPPLYEHLFEVALPKTAYLFVGKKTEELLKKAKGTQDKDCMSSTSPVEVLDRLIQQGADAHTKELNKLSLPSKSADWTHFGGSPPSDEIHTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKATALEEHNAAMKDQLKLQEKEIQALKLSLQKEQARYHQFQEEHEGIVAQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIADLRLELKKANNKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQHKHTDTTKEVEMLHAAFRKELEKAKLCVQQQSQRLDASQKRIAELESQLSKKDHLFLEQKKYLEDVKIKARGQLQAVESRYRAQKRITQAFELEILNLFGRLENSPLKKLEDEKTEAAEAAEERLDCGNEGCADTVAGYSEETIDRNGETKPPSTRGSSKGGSNSELSTPEKKQNQRFSSHWETSMLREPSTTVPLTVGSLPSSKSFLGMKSRELFRNKSESQCDEHGVTINSLSDTLKTELCKDPSMETKAPPSPDNLSLSPKIQESSVGQLHIMDYNETHHDHS

Target ORF information:

RefSeq Version	XM_015279760.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279760.1

ORF Insert Sequence:

ATGGCACAGC AGGGGAATGT TGGTGAGCTC CTCTCAATGC TGGATTCTCC AATTCTGGGT 
GTCCTGGAAG ACATAACAGC TGCATTCAAA GATAATCTCA TTTGTGACCG TGGACCTATG 
CTTGTGAACA GCCTGGTAGA CTATTACTTG GAAACCAATT CTCAGCAGGC ACTGCATATA 
CTGTCCACAT TGCAGGAGCC TCATGACAAG CACCTTCTGG ACAAAATTAA TGAATATATG 
GGCAAAGCTG CTACTCGTTT ACCCACTCTC TCGCTGCTGG GACATGTGAT AAGGCGACAA 
CCATCGTGGA AACATAAGCT TTCTCAAGCA CCTCTCCTAC TTTCATTACT TAAATGTCTC 
AAGACTGACA CAGATGTAGT AGTACTCACC ACAGGTGTCC TAGTGCTAAT AACCATGTTG 
CCAATGATTC CTCAGTCTGG CAAACAGTAC CTCCATGATT TCTTTGGTAT CTTTGGGCGT 
CTCTCAGCCT GGTGCTTGCA GAATCCAGGT CACGTGGCAG AGATTTACCT TGTCCATCTT 
CATGCCAGTG TTTATGCTCT CTTTCATCGG CTTTATGGAA TGTATCCTTG CAATTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACTTGG AGACCTTTGA AGAGGTAGTC 
AAGCCAATGA TGGAACATGT GCGAATTCAT CCAGAATTAG TGACTGGATC TAAGGACCAT 
GAACTGGACC CACGAAGGTG GAAAAGACTA GAAACTCATG ATGTTGTGAT AGAATGTGCC 
AAAATCTCCC TGGACCCTGC AGAAGCCTCA TATGAAGATG GCTATTACTC TGCGTCTCGG 
AAATCCTGTA CAAACTTAAA ACATCATCAA ACTGACCCCA GTGCCAGCCA TTACATTGAC 
ACACAGAGCA GCTATGGGAC GTCTACCCCA TATTCCACTC CTCGGCTAAC ACTATCACAA 
CTGCCAGGGC AGCTACCTCA GATTCTGAGC CCGCAGTCAA TAAAGCTGTC AACTGAGCCA 
CAGCAGGTTA CCATCTGGAG TCCTTCTGCA GTTTGTGGTA TGACCACTCC ACCAACCTCC 
CCTGGAATTG TCCCATCAGA GTCATCCCAG TCAACATCAC AACCTTACAG CAAAGCTTTT 
GGCACATCTG CAGTGGGGAA GGGAACACCT TTGGGGACAC CAGCCACATC TCCTCCACCC 
TGCACTTCAG ATGACTTTGT GCATGTCTCA CTTCCTTCAG CTGCTGCCAC ACCTCCTAAA 
AAGGAGGACA AACCAGATTC TGGGAGGCCT TCACTGTACC GACAGCAAAA TGTCATAAGC 
AGCGATAAAT CATTGGACAC ATCTGGTAGT AAAAGTTCAG TAACCTTAAG TGATCTTCCG 
GAGTTTTTAG GCGGTTTGTC TTTTGAAGAT AGTGCTGAAA AGGACAGAGA AGAAGATGCA 
ATATCTAAAG AGATCTCTGA GATCACGACT GATGCTGAAC ACATGGTGCC TAGAGGAGGA 
TTTGACTCTC CATTTTATCA CACAAATGAA AATCTGTCAG GTTCTCAGAA GAAGACCCAG 
TCAGTAGTCT CTAGTGTGCA GGGACACAGT CTGACCTCTG AGCCTTTAAC GTCTTCTCTG 
GACAAGCCTG GGCCTGAGAG TGCACTGGAG ACACCCAAAC AAACATTTAC TCCCATAGAC 
AAGCCCTGCG GAGGCTCTGG TGAAAGCCCT GCTGGTAACA GGGAAGGAAC CTCTGGGGAG 
ACAAGTATTC TCACTCCCAG CCCTTGCAAA GCATCAGCAC AAGGAAGAGC AGTGTTTGGG 
AGTGGGCAGC CTCCCCTGTA TGAGCACCTT TTTGAGGTTG CGCTACCAAA GACTGCCTAC 
CTCTTTGTTG GCAAGAAGAC TGAGGAGCTG CTAAAGAAAG CCAAGGGAAC CCAGGATAAA 
GACTGCATGT CCTCTACTTC TCCTGTGGAA GTACTGGACA GACTGATACA GCAAGGGGCA 
GATGCACACA CTAAGGAACT GAACAAATTG TCTCTGCCAA GCAAATCTGC TGACTGGACT 
CACTTTGGAG GTTCTCCCCC TTCAGATGAG ATTCACACCC TGCGTAACCA ATTGCTGTTG 
CTGCACAACC AGTTGCTGTA TGAACGCTTC AAAAGACAGC AGCATGCCCT TCGGAACCGG 
CGGCTCTTGC GGAAAGTGAT TAAAGCCACA GCACTGGAGG AACATAATGC TGCCATGAAA 
GATCAGCTGA AATTACAGGA AAAAGAAATC CAGGCCTTGA AACTGAGTCT GCAGAAAGAA 
CAGGCCAGGT ACCACCAGTT TCAGGAGGAG CACGAAGGGA TAGTGGCTCA GCTTCACAGC 
CAGATCAGAC AGCTGCAACA TGACCGTGAG GAATTCTACA ACCAGAGCCA GGAATTGCAG 
ACCAAGCTGG AAGACTGCAG GAACATGATT GCAGATCTGA GGTTAGAATT GAAAAAGGCT 
AACAACAAAG TCTGTCACAC AGAACTGCTT CTTAGCCAAG TTTCTCAAAA GCTTTCCAAC 
AGTGAATCAG TGCAACAGCA GATGGAGTTC TTGAACAGAC AACTTCTGGT TCTTGGAGAG 
GTCAATGAGT TGTACCTGGA GCAGCTGCAG CACAAGCACA CAGACACTAC AAAGGAGGTT 
GAAATGTTGC ATGCTGCTTT TCGAAAGGAA CTGGAGAAAG CAAAATTGTG TGTTCAGCAG 
CAAAGCCAAA GGCTCGATGC TTCTCAGAAA CGGATAGCTG AACTGGAATC TCAGCTTTCT 
AAAAAGGACC ACCTCTTCTT GGAGCAAAAG AAATACCTGG AAGATGTCAA AATCAAAGCA 
AGAGGTCAGT TGCAAGCAGT AGAGAGTAGA TACAGGGCCC AGAAAAGGAT CACCCAGGCG 
TTTGAGCTGG AGATCTTGAA CCTGTTTGGC CGACTGGAGA ACAGCCCGCT GAAAAAACTT 
GAAGATGAAA AAACAGAAGC AGCTGAAGCA GCAGAAGAAA GGCTGGATTG TGGTAATGAA 
GGTTGTGCAG ATACTGTGGC AGGATACAGC GAAGAAACCA TTGATAGAAA TGGAGAAACC 
AAACCTCCCA GCACTCGAGG TAGCAGTAAA GGTGGCAGCA ACAGTGAGCT CTCCACCCCA 
GAAAAAAAAC AGAACCAGAG ATTCAGCAGT CACTGGGAGA CCTCCATGTT GCGGGAGCCC 
TCCACTACTG TCCCACTGAC TGTAGGTTCA CTCCCCAGCT CCAAGAGCTT CCTTGGTATG 
AAGTCACGAG AGTTGTTTCG CAACAAAAGT GAGAGCCAGT GTGATGAGCA TGGTGTAACC 
ATCAACAGCC TTTCTGATAC TCTAAAGACT GAACTATGTA AAGATCCAAG CATGGAGACG 
AAGGCTCCTC CAAGCCCCGA TAACCTTAGC CTCTCACCTA AGATCCAGGA AAGCAGTGTT 
GGACAGCTTC ATATCATGGA CTACAATGAA ACTCATCATG ACCACAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_415449.6 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_415449.2 Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X1, mRNA.

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CloneID	OGa43672
Clone ID Related Accession (Same CDS sequence)	XM_015279760.1 , XM_415449.6 , XM_415449.5 , XM_015279760.2
Accession Version	XM_415449.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_415449.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGC AGGGGAATGT TGGTGAGCTC CTCTCAATGC TGGATTCTCC AATTCTGGGT 
GTCCTGGAAG ACATAACAGC TGCATTCAAA GATAATCTCA TTTGTGACCG TGGACCTATG 
CTTGTGAACA GCCTGGTAGA CTATTACTTG GAAACCAATT CTCAGCAGGC ACTGCATATA 
CTGTCCACAT TGCAGGAGCC TCATGACAAG CACCTTCTGG ACAAAATTAA TGAATATATG 
GGCAAAGCTG CTACTCGTTT ACCCACTCTC TCGCTGCTGG GACATGTGAT AAGGCGACAA 
CCATCGTGGA AACATAAGCT TTCTCAAGCA CCTCTCCTAC TTTCATTACT TAAATGTCTC 
AAGACTGACA CAGATGTAGT AGTACTCACC ACAGGTGTCC TAGTGCTAAT AACCATGTTG 
CCAATGATTC CTCAGTCTGG CAAACAGTAC CTCCATGATT TCTTTGGTAT CTTTGGGCGT 
CTCTCAGCCT GGTGCTTGCA GAATCCAGGT CACGTGGCAG AGATTTACCT TGTCCATCTT 
CATGCCAGTG TTTATGCTCT CTTTCATCGG CTTTATGGAA TGTATCCTTG CAATTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACTTGG AGACCTTTGA AGAGGTAGTC 
AAGCCAATGA TGGAACATGT GCGAATTCAT CCAGAATTAG TGACTGGATC TAAGGACCAT 
GAACTGGACC CACGAAGGTG GAAAAGACTA GAAACTCATG ATGTTGTGAT AGAATGTGCC 
AAAATCTCCC TGGACCCTGC AGAAGCCTCA TATGAAGATG GCTATTACTC TGCGTCTCGG 
AAATCCTGTA CAAACTTAAA ACATCATCAA ACTGACCCCA GTGCCAGCCA TTACATTGAC 
ACACAGAGCA GCTATGGGAC GTCTACCCCA TATTCCACTC CTCGGCTAAC ACTATCACAA 
CTGCCAGGGC AGCTACCTCA GATTCTGAGC CCGCAGTCAA TAAAGCTGTC AACTGAGCCA 
CAGCAGGTTA CCATCTGGAG TCCTTCTGCA GTTTGTGGTA TGACCACTCC ACCAACCTCC 
CCTGGAATTG TCCCATCAGA GTCATCCCAG TCAACATCAC AACCTTACAG CAAAGCTTTT 
GGCACATCTG CAGTGGGGAA GGGAACACCT TTGGGGACAC CAGCCACATC TCCTCCACCC 
TGCACTTCAG ATGACTTTGT GCATGTCTCA CTTCCTTCAG CTGCTGCCAC ACCTCCTAAA 
AAGGAGGACA AACCAGATTC TGGGAGGCCT TCACTGTACC GACAGCAAAA TGTCATAAGC 
AGCGATAAAT CATTGGACAC ATCTGGTAGT AAAAGTTCAG TAACCTTAAG TGATCTTCCG 
GAGTTTTTAG GCGGTTTGTC TTTTGAAGAT AGTGCTGAAA AGGACAGAGA AGAAGATGCA 
ATATCTAAAG AGATCTCTGA GATCACGACT GATGCTGAAC ACATGGTGCC TAGAGGAGGA 
TTTGACTCTC CATTTTATCA CACAAATGAA AATCTGTCAG GTTCTCAGAA GAAGACCCAG 
TCAGTAGTCT CTAGTGTGCA GGGACACAGT CTGACCTCTG AGCCTTTAAC GTCTTCTCTG 
GACAAGCCTG GGCCTGAGAG TGCACTGGAG ACACCCAAAC AAACATTTAC TCCCATAGAC 
AAGCCCTGCG GAGGCTCTGG TGAAAGCCCT GCTGGTAACA GGGAAGGAAC CTCTGGGGAG 
ACAAGTATTC TCACTCCCAG CCCTTGCAAA GCATCAGCAC AAGGAAGAGC AGTGTTTGGG 
AGTGGGCAGC CTCCCCTGTA TGAGCACCTT TTTGAGGTTG CGCTACCAAA GACTGCCTAC 
CTCTTTGTTG GCAAGAAGAC TGAGGAGCTG CTAAAGAAAG CCAAGGGAAC CCAGGATAAA 
GACTGCATGT CCTCTACTTC TCCTGTGGAA GTACTGGACA GACTGATACA GCAAGGGGCA 
GATGCACACA CTAAGGAACT GAACAAATTG TCTCTGCCAA GCAAATCTGC TGACTGGACT 
CACTTTGGAG GTTCTCCCCC TTCAGATGAG ATTCACACCC TGCGTAACCA ATTGCTGTTG 
CTGCACAACC AGTTGCTGTA TGAACGCTTC AAAAGACAGC AGCATGCCCT TCGGAACCGG 
CGGCTCTTGC GGAAAGTGAT TAAAGCCACA GCACTGGAGG AACATAATGC TGCCATGAAA 
GATCAGCTGA AATTACAGGA AAAAGAAATC CAGGCCTTGA AACTGAGTCT GCAGAAAGAA 
CAGGCCAGGT ACCACCAGTT TCAGGAGGAG CACGAAGGGA TAGTGGCTCA GCTTCACAGC 
CAGATCAGAC AGCTGCAACA TGACCGTGAG GAATTCTACA ACCAGAGCCA GGAATTGCAG 
ACCAAGCTGG AAGACTGCAG GAACATGATT GCAGATCTGA GGTTAGAATT GAAAAAGGCT 
AACAACAAAG TCTGTCACAC AGAACTGCTT CTTAGCCAAG TTTCTCAAAA GCTTTCCAAC 
AGTGAATCAG TGCAACAGCA GATGGAGTTC TTGAACAGAC AACTTCTGGT TCTTGGAGAG 
GTCAATGAGT TGTACCTGGA GCAGCTGCAG CACAAGCACA CAGACACTAC AAAGGAGGTT 
GAAATGTTGC ATGCTGCTTT TCGAAAGGAA CTGGAGAAAG CAAAATTGTG TGTTCAGCAG 
CAAAGCCAAA GGCTCGATGC TTCTCAGAAA CGGATAGCTG AACTGGAATC TCAGCTTTCT 
AAAAAGGACC ACCTCTTCTT GGAGCAAAAG AAATACCTGG AAGATGTCAA AATCAAAGCA 
AGAGGTCAGT TGCAAGCAGT AGAGAGTAGA TACAGGGCCC AGAAAAGGAT CACCCAGGCG 
TTTGAGCTGG AGATCTTGAA CCTGTTTGGC CGACTGGAGA ACAGCCCGCT GAAAAAACTT 
GAAGATGAAA AAACAGAAGC AGCTGAAGCA GCAGAAGAAA GGCTGGATTG TGGTAATGAA 
GGTTGTGCAG ATACTGTGGC AGGATACAGC GAAGAAACCA TTGATAGAAA TGGAGAAACC 
AAACCTCCCA GCACTCGAGG TAGCAGTAAA GGTGGCAGCA ACAGTGAGCT CTCCACCCCA 
GAAAAAAAAC AGAACCAGAG ATTCAGCAGT CACTGGGAGA CCTCCATGTT GCGGGAGCCC 
TCCACTACTG TCCCACTGAC TGTAGGTTCA CTCCCCAGCT CCAAGAGCTT CCTTGGTATG 
AAGTCACGAG AGTTGTTTCG CAACAAAAGT GAGAGCCAGT GTGATGAGCA TGGTGTAACC 
ATCAACAGCC TTTCTGATAC TCTAAAGACT GAACTATGTA AAGATCCAAG CATGGAGACG 
AAGGCTCCTC CAAGCCCCGA TAACCTTAGC CTCTCACCTA AGATCCAGGA AAGCAGTGTT 
GGACAGCTTC ATATCATGGA CTACAATGAA ACTCATCATG ACCACAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_415449.2
CDS	224..3694
Translation	MAQQGNVGELLSMLDSPILGVLEDITAAFKDNLICDRGPMLVNSLVDYYLETNSQQALHILSTLQEPHDKHLLDKINEYMGKAATRLPTLSLLGHVIRRQPSWKHKLSQAPLLLSLLKCLKTDTDVVVLTTGVLVLITMLPMIPQSGKQYLHDFFGIFGRLSAWCLQNPGHVAEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPAEASYEDGYYSASRKSCTNLKHHQTDPSASHYIDTQSSYGTSTPYSTPRLTLSQLPGQLPQILSPQSIKLSTEPQQVTIWSPSAVCGMTTPPTSPGIVPSESSQSTSQPYSKAFGTSAVGKGTPLGTPATSPPPCTSDDFVHVSLPSAAATPPKKEDKPDSGRPSLYRQQNVISSDKSLDTSGSKSSVTLSDLPEFLGGLSFEDSAEKDREEDAISKEISEITTDAEHMVPRGGFDSPFYHTNENLSGSQKKTQSVVSSVQGHSLTSEPLTSSLDKPGPESALETPKQTFTPIDKPCGGSGESPAGNREGTSGETSILTPSPCKASAQGRAVFGSGQPPLYEHLFEVALPKTAYLFVGKKTEELLKKAKGTQDKDCMSSTSPVEVLDRLIQQGADAHTKELNKLSLPSKSADWTHFGGSPPSDEIHTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKATALEEHNAAMKDQLKLQEKEIQALKLSLQKEQARYHQFQEEHEGIVAQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIADLRLELKKANNKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQHKHTDTTKEVEMLHAAFRKELEKAKLCVQQQSQRLDASQKRIAELESQLSKKDHLFLEQKKYLEDVKIKARGQLQAVESRYRAQKRITQAFELEILNLFGRLENSPLKKLEDEKTEAAEAAEERLDCGNEGCADTVAGYSEETIDRNGETKPPSTRGSSKGGSNSELSTPEKKQNQRFSSHWETSMLREPSTTVPLTVGSLPSSKSFLGMKSRELFRNKSESQCDEHGVTINSLSDTLKTELCKDPSMETKAPPSPDNLSLSPKIQESSVGQLHIMDYNETHHDHS

Target ORF information:

RefSeq Version	XM_415449.6
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_415449.6

ORF Insert Sequence:

ATGGCACAGC AGGGGAATGT TGGTGAGCTC CTCTCAATGC TGGATTCTCC AATTCTGGGT 
GTCCTGGAAG ACATAACAGC TGCATTCAAA GATAATCTCA TTTGTGACCG TGGACCTATG 
CTTGTGAACA GCCTGGTAGA CTATTACTTG GAAACCAATT CTCAGCAGGC ACTGCATATA 
CTGTCCACAT TGCAGGAGCC TCATGACAAG CACCTTCTGG ACAAAATTAA TGAATATATG 
GGCAAAGCTG CTACTCGTTT ACCCACTCTC TCGCTGCTGG GACATGTGAT AAGGCGACAA 
CCATCGTGGA AACATAAGCT TTCTCAAGCA CCTCTCCTAC TTTCATTACT TAAATGTCTC 
AAGACTGACA CAGATGTAGT AGTACTCACC ACAGGTGTCC TAGTGCTAAT AACCATGTTG 
CCAATGATTC CTCAGTCTGG CAAACAGTAC CTCCATGATT TCTTTGGTAT CTTTGGGCGT 
CTCTCAGCCT GGTGCTTGCA GAATCCAGGT CACGTGGCAG AGATTTACCT TGTCCATCTT 
CATGCCAGTG TTTATGCTCT CTTTCATCGG CTTTATGGAA TGTATCCTTG CAATTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACTTGG AGACCTTTGA AGAGGTAGTC 
AAGCCAATGA TGGAACATGT GCGAATTCAT CCAGAATTAG TGACTGGATC TAAGGACCAT 
GAACTGGACC CACGAAGGTG GAAAAGACTA GAAACTCATG ATGTTGTGAT AGAATGTGCC 
AAAATCTCCC TGGACCCTGC AGAAGCCTCA TATGAAGATG GCTATTACTC TGCGTCTCGG 
AAATCCTGTA CAAACTTAAA ACATCATCAA ACTGACCCCA GTGCCAGCCA TTACATTGAC 
ACACAGAGCA GCTATGGGAC GTCTACCCCA TATTCCACTC CTCGGCTAAC ACTATCACAA 
CTGCCAGGGC AGCTACCTCA GATTCTGAGC CCGCAGTCAA TAAAGCTGTC AACTGAGCCA 
CAGCAGGTTA CCATCTGGAG TCCTTCTGCA GTTTGTGGTA TGACCACTCC ACCAACCTCC 
CCTGGAATTG TCCCATCAGA GTCATCCCAG TCAACATCAC AACCTTACAG CAAAGCTTTT 
GGCACATCTG CAGTGGGGAA GGGAACACCT TTGGGGACAC CAGCCACATC TCCTCCACCC 
TGCACTTCAG ATGACTTTGT GCATGTCTCA CTTCCTTCAG CTGCTGCCAC ACCTCCTAAA 
AAGGAGGACA AACCAGATTC TGGGAGGCCT TCACTGTACC GACAGCAAAA TGTCATAAGC 
AGCGATAAAT CATTGGACAC ATCTGGTAGT AAAAGTTCAG TAACCTTAAG TGATCTTCCG 
GAGTTTTTAG GCGGTTTGTC TTTTGAAGAT AGTGCTGAAA AGGACAGAGA AGAAGATGCA 
ATATCTAAAG AGATCTCTGA GATCACGACT GATGCTGAAC ACATGGTGCC TAGAGGAGGA 
TTTGACTCTC CATTTTATCA CACAAATGAA AATCTGTCAG GTTCTCAGAA GAAGACCCAG 
TCAGTAGTCT CTAGTGTGCA GGGACACAGT CTGACCTCTG AGCCTTTAAC GTCTTCTCTG 
GACAAGCCTG GGCCTGAGAG TGCACTGGAG ACACCCAAAC AAACATTTAC TCCCATAGAC 
AAGCCCTGCG GAGGCTCTGG TGAAAGCCCT GCTGGTAACA GGGAAGGAAC CTCTGGGGAG 
ACAAGTATTC TCACTCCCAG CCCTTGCAAA GCATCAGCAC AAGGAAGAGC AGTGTTTGGG 
AGTGGGCAGC CTCCCCTGTA TGAGCACCTT TTTGAGGTTG CGCTACCAAA GACTGCCTAC 
CTCTTTGTTG GCAAGAAGAC TGAGGAGCTG CTAAAGAAAG CCAAGGGAAC CCAGGATAAA 
GACTGCATGT CCTCTACTTC TCCTGTGGAA GTACTGGACA GACTGATACA GCAAGGGGCA 
GATGCACACA CTAAGGAACT GAACAAATTG TCTCTGCCAA GCAAATCTGC TGACTGGACT 
CACTTTGGAG GTTCTCCCCC TTCAGATGAG ATTCACACCC TGCGTAACCA ATTGCTGTTG 
CTGCACAACC AGTTGCTGTA TGAACGCTTC AAAAGACAGC AGCATGCCCT TCGGAACCGG 
CGGCTCTTGC GGAAAGTGAT TAAAGCCACA GCACTGGAGG AACATAATGC TGCCATGAAA 
GATCAGCTGA AATTACAGGA AAAAGAAATC CAGGCCTTGA AACTGAGTCT GCAGAAAGAA 
CAGGCCAGGT ACCACCAGTT TCAGGAGGAG CACGAAGGGA TAGTGGCTCA GCTTCACAGC 
CAGATCAGAC AGCTGCAACA TGACCGTGAG GAATTCTACA ACCAGAGCCA GGAATTGCAG 
ACCAAGCTGG AAGACTGCAG GAACATGATT GCAGATCTGA GGTTAGAATT GAAAAAGGCT 
AACAACAAAG TCTGTCACAC AGAACTGCTT CTTAGCCAAG TTTCTCAAAA GCTTTCCAAC 
AGTGAATCAG TGCAACAGCA GATGGAGTTC TTGAACAGAC AACTTCTGGT TCTTGGAGAG 
GTCAATGAGT TGTACCTGGA GCAGCTGCAG CACAAGCACA CAGACACTAC AAAGGAGGTT 
GAAATGTTGC ATGCTGCTTT TCGAAAGGAA CTGGAGAAAG CAAAATTGTG TGTTCAGCAG 
CAAAGCCAAA GGCTCGATGC TTCTCAGAAA CGGATAGCTG AACTGGAATC TCAGCTTTCT 
AAAAAGGACC ACCTCTTCTT GGAGCAAAAG AAATACCTGG AAGATGTCAA AATCAAAGCA 
AGAGGTCAGT TGCAAGCAGT AGAGAGTAGA TACAGGGCCC AGAAAAGGAT CACCCAGGCG 
TTTGAGCTGG AGATCTTGAA CCTGTTTGGC CGACTGGAGA ACAGCCCGCT GAAAAAACTT 
GAAGATGAAA AAACAGAAGC AGCTGAAGCA GCAGAAGAAA GGCTGGATTG TGGTAATGAA 
GGTTGTGCAG ATACTGTGGC AGGATACAGC GAAGAAACCA TTGATAGAAA TGGAGAAACC 
AAACCTCCCA GCACTCGAGG TAGCAGTAAA GGTGGCAGCA ACAGTGAGCT CTCCACCCCA 
GAAAAAAAAC AGAACCAGAG ATTCAGCAGT CACTGGGAGA CCTCCATGTT GCGGGAGCCC 
TCCACTACTG TCCCACTGAC TGTAGGTTCA CTCCCCAGCT CCAAGAGCTT CCTTGGTATG 
AAGTCACGAG AGTTGTTTCG CAACAAAAGT GAGAGCCAGT GTGATGAGCA TGGTGTAACC 
ATCAACAGCC TTTCTGATAC TCTAAAGACT GAACTATGTA AAGATCCAAG CATGGAGACG 
AAGGCTCCTC CAAGCCCCGA TAACCTTAGC CTCTCACCTA AGATCCAGGA AAGCAGTGTT 
GGACAGCTTC ATATCATGGA CTACAATGAA ACTCATCATG ACCACAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_415449.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_415449.2 Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X1, mRNA.

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CloneID	OGa43672
Clone ID Related Accession (Same CDS sequence)	XM_015279760.1 , XM_415449.6 , XM_415449.5 , XM_015279760.2
Accession Version	XM_415449.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_415449.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGC AGGGGAATGT TGGTGAGCTC CTCTCAATGC TGGATTCTCC AATTCTGGGT 
GTCCTGGAAG ACATAACAGC TGCATTCAAA GATAATCTCA TTTGTGACCG TGGACCTATG 
CTTGTGAACA GCCTGGTAGA CTATTACTTG GAAACCAATT CTCAGCAGGC ACTGCATATA 
CTGTCCACAT TGCAGGAGCC TCATGACAAG CACCTTCTGG ACAAAATTAA TGAATATATG 
GGCAAAGCTG CTACTCGTTT ACCCACTCTC TCGCTGCTGG GACATGTGAT AAGGCGACAA 
CCATCGTGGA AACATAAGCT TTCTCAAGCA CCTCTCCTAC TTTCATTACT TAAATGTCTC 
AAGACTGACA CAGATGTAGT AGTACTCACC ACAGGTGTCC TAGTGCTAAT AACCATGTTG 
CCAATGATTC CTCAGTCTGG CAAACAGTAC CTCCATGATT TCTTTGGTAT CTTTGGGCGT 
CTCTCAGCCT GGTGCTTGCA GAATCCAGGT CACGTGGCAG AGATTTACCT TGTCCATCTT 
CATGCCAGTG TTTATGCTCT CTTTCATCGG CTTTATGGAA TGTATCCTTG CAATTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACTTGG AGACCTTTGA AGAGGTAGTC 
AAGCCAATGA TGGAACATGT GCGAATTCAT CCAGAATTAG TGACTGGATC TAAGGACCAT 
GAACTGGACC CACGAAGGTG GAAAAGACTA GAAACTCATG ATGTTGTGAT AGAATGTGCC 
AAAATCTCCC TGGACCCTGC AGAAGCCTCA TATGAAGATG GCTATTACTC TGCGTCTCGG 
AAATCCTGTA CAAACTTAAA ACATCATCAA ACTGACCCCA GTGCCAGCCA TTACATTGAC 
ACACAGAGCA GCTATGGGAC GTCTACCCCA TATTCCACTC CTCGGCTAAC ACTATCACAA 
CTGCCAGGGC AGCTACCTCA GATTCTGAGC CCGCAGTCAA TAAAGCTGTC AACTGAGCCA 
CAGCAGGTTA CCATCTGGAG TCCTTCTGCA GTTTGTGGTA TGACCACTCC ACCAACCTCC 
CCTGGAATTG TCCCATCAGA GTCATCCCAG TCAACATCAC AACCTTACAG CAAAGCTTTT 
GGCACATCTG CAGTGGGGAA GGGAACACCT TTGGGGACAC CAGCCACATC TCCTCCACCC 
TGCACTTCAG ATGACTTTGT GCATGTCTCA CTTCCTTCAG CTGCTGCCAC ACCTCCTAAA 
AAGGAGGACA AACCAGATTC TGGGAGGCCT TCACTGTACC GACAGCAAAA TGTCATAAGC 
AGCGATAAAT CATTGGACAC ATCTGGTAGT AAAAGTTCAG TAACCTTAAG TGATCTTCCG 
GAGTTTTTAG GCGGTTTGTC TTTTGAAGAT AGTGCTGAAA AGGACAGAGA AGAAGATGCA 
ATATCTAAAG AGATCTCTGA GATCACGACT GATGCTGAAC ACATGGTGCC TAGAGGAGGA 
TTTGACTCTC CATTTTATCA CACAAATGAA AATCTGTCAG GTTCTCAGAA GAAGACCCAG 
TCAGTAGTCT CTAGTGTGCA GGGACACAGT CTGACCTCTG AGCCTTTAAC GTCTTCTCTG 
GACAAGCCTG GGCCTGAGAG TGCACTGGAG ACACCCAAAC AAACATTTAC TCCCATAGAC 
AAGCCCTGCG GAGGCTCTGG TGAAAGCCCT GCTGGTAACA GGGAAGGAAC CTCTGGGGAG 
ACAAGTATTC TCACTCCCAG CCCTTGCAAA GCATCAGCAC AAGGAAGAGC AGTGTTTGGG 
AGTGGGCAGC CTCCCCTGTA TGAGCACCTT TTTGAGGTTG CGCTACCAAA GACTGCCTAC 
CTCTTTGTTG GCAAGAAGAC TGAGGAGCTG CTAAAGAAAG CCAAGGGAAC CCAGGATAAA 
GACTGCATGT CCTCTACTTC TCCTGTGGAA GTACTGGACA GACTGATACA GCAAGGGGCA 
GATGCACACA CTAAGGAACT GAACAAATTG TCTCTGCCAA GCAAATCTGC TGACTGGACT 
CACTTTGGAG GTTCTCCCCC TTCAGATGAG ATTCACACCC TGCGTAACCA ATTGCTGTTG 
CTGCACAACC AGTTGCTGTA TGAACGCTTC AAAAGACAGC AGCATGCCCT TCGGAACCGG 
CGGCTCTTGC GGAAAGTGAT TAAAGCCACA GCACTGGAGG AACATAATGC TGCCATGAAA 
GATCAGCTGA AATTACAGGA AAAAGAAATC CAGGCCTTGA AACTGAGTCT GCAGAAAGAA 
CAGGCCAGGT ACCACCAGTT TCAGGAGGAG CACGAAGGGA TAGTGGCTCA GCTTCACAGC 
CAGATCAGAC AGCTGCAACA TGACCGTGAG GAATTCTACA ACCAGAGCCA GGAATTGCAG 
ACCAAGCTGG AAGACTGCAG GAACATGATT GCAGATCTGA GGTTAGAATT GAAAAAGGCT 
AACAACAAAG TCTGTCACAC AGAACTGCTT CTTAGCCAAG TTTCTCAAAA GCTTTCCAAC 
AGTGAATCAG TGCAACAGCA GATGGAGTTC TTGAACAGAC AACTTCTGGT TCTTGGAGAG 
GTCAATGAGT TGTACCTGGA GCAGCTGCAG CACAAGCACA CAGACACTAC AAAGGAGGTT 
GAAATGTTGC ATGCTGCTTT TCGAAAGGAA CTGGAGAAAG CAAAATTGTG TGTTCAGCAG 
CAAAGCCAAA GGCTCGATGC TTCTCAGAAA CGGATAGCTG AACTGGAATC TCAGCTTTCT 
AAAAAGGACC ACCTCTTCTT GGAGCAAAAG AAATACCTGG AAGATGTCAA AATCAAAGCA 
AGAGGTCAGT TGCAAGCAGT AGAGAGTAGA TACAGGGCCC AGAAAAGGAT CACCCAGGCG 
TTTGAGCTGG AGATCTTGAA CCTGTTTGGC CGACTGGAGA ACAGCCCGCT GAAAAAACTT 
GAAGATGAAA AAACAGAAGC AGCTGAAGCA GCAGAAGAAA GGCTGGATTG TGGTAATGAA 
GGTTGTGCAG ATACTGTGGC AGGATACAGC GAAGAAACCA TTGATAGAAA TGGAGAAACC 
AAACCTCCCA GCACTCGAGG TAGCAGTAAA GGTGGCAGCA ACAGTGAGCT CTCCACCCCA 
GAAAAAAAAC AGAACCAGAG ATTCAGCAGT CACTGGGAGA CCTCCATGTT GCGGGAGCCC 
TCCACTACTG TCCCACTGAC TGTAGGTTCA CTCCCCAGCT CCAAGAGCTT CCTTGGTATG 
AAGTCACGAG AGTTGTTTCG CAACAAAAGT GAGAGCCAGT GTGATGAGCA TGGTGTAACC 
ATCAACAGCC TTTCTGATAC TCTAAAGACT GAACTATGTA AAGATCCAAG CATGGAGACG 
AAGGCTCCTC CAAGCCCCGA TAACCTTAGC CTCTCACCTA AGATCCAGGA AAGCAGTGTT 
GGACAGCTTC ATATCATGGA CTACAATGAA ACTCATCATG ACCACAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_415449.2
CDS	219..3689
Translation	MAQQGNVGELLSMLDSPILGVLEDITAAFKDNLICDRGPMLVNSLVDYYLETNSQQALHILSTLQEPHDKHLLDKINEYMGKAATRLPTLSLLGHVIRRQPSWKHKLSQAPLLLSLLKCLKTDTDVVVLTTGVLVLITMLPMIPQSGKQYLHDFFGIFGRLSAWCLQNPGHVAEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPAEASYEDGYYSASRKSCTNLKHHQTDPSASHYIDTQSSYGTSTPYSTPRLTLSQLPGQLPQILSPQSIKLSTEPQQVTIWSPSAVCGMTTPPTSPGIVPSESSQSTSQPYSKAFGTSAVGKGTPLGTPATSPPPCTSDDFVHVSLPSAAATPPKKEDKPDSGRPSLYRQQNVISSDKSLDTSGSKSSVTLSDLPEFLGGLSFEDSAEKDREEDAISKEISEITTDAEHMVPRGGFDSPFYHTNENLSGSQKKTQSVVSSVQGHSLTSEPLTSSLDKPGPESALETPKQTFTPIDKPCGGSGESPAGNREGTSGETSILTPSPCKASAQGRAVFGSGQPPLYEHLFEVALPKTAYLFVGKKTEELLKKAKGTQDKDCMSSTSPVEVLDRLIQQGADAHTKELNKLSLPSKSADWTHFGGSPPSDEIHTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKATALEEHNAAMKDQLKLQEKEIQALKLSLQKEQARYHQFQEEHEGIVAQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIADLRLELKKANNKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQHKHTDTTKEVEMLHAAFRKELEKAKLCVQQQSQRLDASQKRIAELESQLSKKDHLFLEQKKYLEDVKIKARGQLQAVESRYRAQKRITQAFELEILNLFGRLENSPLKKLEDEKTEAAEAAEERLDCGNEGCADTVAGYSEETIDRNGETKPPSTRGSSKGGSNSELSTPEKKQNQRFSSHWETSMLREPSTTVPLTVGSLPSSKSFLGMKSRELFRNKSESQCDEHGVTINSLSDTLKTELCKDPSMETKAPPSPDNLSLSPKIQESSVGQLHIMDYNETHHDHS

Target ORF information:

RefSeq Version	XM_415449.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_415449.5

ORF Insert Sequence:

ATGGCACAGC AGGGGAATGT TGGTGAGCTC CTCTCAATGC TGGATTCTCC AATTCTGGGT 
GTCCTGGAAG ACATAACAGC TGCATTCAAA GATAATCTCA TTTGTGACCG TGGACCTATG 
CTTGTGAACA GCCTGGTAGA CTATTACTTG GAAACCAATT CTCAGCAGGC ACTGCATATA 
CTGTCCACAT TGCAGGAGCC TCATGACAAG CACCTTCTGG ACAAAATTAA TGAATATATG 
GGCAAAGCTG CTACTCGTTT ACCCACTCTC TCGCTGCTGG GACATGTGAT AAGGCGACAA 
CCATCGTGGA AACATAAGCT TTCTCAAGCA CCTCTCCTAC TTTCATTACT TAAATGTCTC 
AAGACTGACA CAGATGTAGT AGTACTCACC ACAGGTGTCC TAGTGCTAAT AACCATGTTG 
CCAATGATTC CTCAGTCTGG CAAACAGTAC CTCCATGATT TCTTTGGTAT CTTTGGGCGT 
CTCTCAGCCT GGTGCTTGCA GAATCCAGGT CACGTGGCAG AGATTTACCT TGTCCATCTT 
CATGCCAGTG TTTATGCTCT CTTTCATCGG CTTTATGGAA TGTATCCTTG CAATTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACTTGG AGACCTTTGA AGAGGTAGTC 
AAGCCAATGA TGGAACATGT GCGAATTCAT CCAGAATTAG TGACTGGATC TAAGGACCAT 
GAACTGGACC CACGAAGGTG GAAAAGACTA GAAACTCATG ATGTTGTGAT AGAATGTGCC 
AAAATCTCCC TGGACCCTGC AGAAGCCTCA TATGAAGATG GCTATTACTC TGCGTCTCGG 
AAATCCTGTA CAAACTTAAA ACATCATCAA ACTGACCCCA GTGCCAGCCA TTACATTGAC 
ACACAGAGCA GCTATGGGAC GTCTACCCCA TATTCCACTC CTCGGCTAAC ACTATCACAA 
CTGCCAGGGC AGCTACCTCA GATTCTGAGC CCGCAGTCAA TAAAGCTGTC AACTGAGCCA 
CAGCAGGTTA CCATCTGGAG TCCTTCTGCA GTTTGTGGTA TGACCACTCC ACCAACCTCC 
CCTGGAATTG TCCCATCAGA GTCATCCCAG TCAACATCAC AACCTTACAG CAAAGCTTTT 
GGCACATCTG CAGTGGGGAA GGGAACACCT TTGGGGACAC CAGCCACATC TCCTCCACCC 
TGCACTTCAG ATGACTTTGT GCATGTCTCA CTTCCTTCAG CTGCTGCCAC ACCTCCTAAA 
AAGGAGGACA AACCAGATTC TGGGAGGCCT TCACTGTACC GACAGCAAAA TGTCATAAGC 
AGCGATAAAT CATTGGACAC ATCTGGTAGT AAAAGTTCAG TAACCTTAAG TGATCTTCCG 
GAGTTTTTAG GCGGTTTGTC TTTTGAAGAT AGTGCTGAAA AGGACAGAGA AGAAGATGCA 
ATATCTAAAG AGATCTCTGA GATCACGACT GATGCTGAAC ACATGGTGCC TAGAGGAGGA 
TTTGACTCTC CATTTTATCA CACAAATGAA AATCTGTCAG GTTCTCAGAA GAAGACCCAG 
TCAGTAGTCT CTAGTGTGCA GGGACACAGT CTGACCTCTG AGCCTTTAAC GTCTTCTCTG 
GACAAGCCTG GGCCTGAGAG TGCACTGGAG ACACCCAAAC AAACATTTAC TCCCATAGAC 
AAGCCCTGCG GAGGCTCTGG TGAAAGCCCT GCTGGTAACA GGGAAGGAAC CTCTGGGGAG 
ACAAGTATTC TCACTCCCAG CCCTTGCAAA GCATCAGCAC AAGGAAGAGC AGTGTTTGGG 
AGTGGGCAGC CTCCCCTGTA TGAGCACCTT TTTGAGGTTG CGCTACCAAA GACTGCCTAC 
CTCTTTGTTG GCAAGAAGAC TGAGGAGCTG CTAAAGAAAG CCAAGGGAAC CCAGGATAAA 
GACTGCATGT CCTCTACTTC TCCTGTGGAA GTACTGGACA GACTGATACA GCAAGGGGCA 
GATGCACACA CTAAGGAACT GAACAAATTG TCTCTGCCAA GCAAATCTGC TGACTGGACT 
CACTTTGGAG GTTCTCCCCC TTCAGATGAG ATTCACACCC TGCGTAACCA ATTGCTGTTG 
CTGCACAACC AGTTGCTGTA TGAACGCTTC AAAAGACAGC AGCATGCCCT TCGGAACCGG 
CGGCTCTTGC GGAAAGTGAT TAAAGCCACA GCACTGGAGG AACATAATGC TGCCATGAAA 
GATCAGCTGA AATTACAGGA AAAAGAAATC CAGGCCTTGA AACTGAGTCT GCAGAAAGAA 
CAGGCCAGGT ACCACCAGTT TCAGGAGGAG CACGAAGGGA TAGTGGCTCA GCTTCACAGC 
CAGATCAGAC AGCTGCAACA TGACCGTGAG GAATTCTACA ACCAGAGCCA GGAATTGCAG 
ACCAAGCTGG AAGACTGCAG GAACATGATT GCAGATCTGA GGTTAGAATT GAAAAAGGCT 
AACAACAAAG TCTGTCACAC AGAACTGCTT CTTAGCCAAG TTTCTCAAAA GCTTTCCAAC 
AGTGAATCAG TGCAACAGCA GATGGAGTTC TTGAACAGAC AACTTCTGGT TCTTGGAGAG 
GTCAATGAGT TGTACCTGGA GCAGCTGCAG CACAAGCACA CAGACACTAC AAAGGAGGTT 
GAAATGTTGC ATGCTGCTTT TCGAAAGGAA CTGGAGAAAG CAAAATTGTG TGTTCAGCAG 
CAAAGCCAAA GGCTCGATGC TTCTCAGAAA CGGATAGCTG AACTGGAATC TCAGCTTTCT 
AAAAAGGACC ACCTCTTCTT GGAGCAAAAG AAATACCTGG AAGATGTCAA AATCAAAGCA 
AGAGGTCAGT TGCAAGCAGT AGAGAGTAGA TACAGGGCCC AGAAAAGGAT CACCCAGGCG 
TTTGAGCTGG AGATCTTGAA CCTGTTTGGC CGACTGGAGA ACAGCCCGCT GAAAAAACTT 
GAAGATGAAA AAACAGAAGC AGCTGAAGCA GCAGAAGAAA GGCTGGATTG TGGTAATGAA 
GGTTGTGCAG ATACTGTGGC AGGATACAGC GAAGAAACCA TTGATAGAAA TGGAGAAACC 
AAACCTCCCA GCACTCGAGG TAGCAGTAAA GGTGGCAGCA ACAGTGAGCT CTCCACCCCA 
GAAAAAAAAC AGAACCAGAG ATTCAGCAGT CACTGGGAGA CCTCCATGTT GCGGGAGCCC 
TCCACTACTG TCCCACTGAC TGTAGGTTCA CTCCCCAGCT CCAAGAGCTT CCTTGGTATG 
AAGTCACGAG AGTTGTTTCG CAACAAAAGT GAGAGCCAGT GTGATGAGCA TGGTGTAACC 
ATCAACAGCC TTTCTGATAC TCTAAAGACT GAACTATGTA AAGATCCAAG CATGGAGACG 
AAGGCTCCTC CAAGCCCCGA TAACCTTAGC CTCTCACCTA AGATCCAGGA AAGCAGTGTT 
GGACAGCTTC ATATCATGGA CTACAATGAA ACTCATCATG ACCACAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_015279760.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135246.1 Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X2, mRNA.

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CloneID	OGa43672
Clone ID Related Accession (Same CDS sequence)	XM_015279760.1 , XM_415449.6 , XM_415449.5 , XM_015279760.2
Accession Version	XM_015279760.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279760.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGC AGGGGAATGT TGGTGAGCTC CTCTCAATGC TGGATTCTCC AATTCTGGGT 
GTCCTGGAAG ACATAACAGC TGCATTCAAA GATAATCTCA TTTGTGACCG TGGACCTATG 
CTTGTGAACA GCCTGGTAGA CTATTACTTG GAAACCAATT CTCAGCAGGC ACTGCATATA 
CTGTCCACAT TGCAGGAGCC TCATGACAAG CACCTTCTGG ACAAAATTAA TGAATATATG 
GGCAAAGCTG CTACTCGTTT ACCCACTCTC TCGCTGCTGG GACATGTGAT AAGGCGACAA 
CCATCGTGGA AACATAAGCT TTCTCAAGCA CCTCTCCTAC TTTCATTACT TAAATGTCTC 
AAGACTGACA CAGATGTAGT AGTACTCACC ACAGGTGTCC TAGTGCTAAT AACCATGTTG 
CCAATGATTC CTCAGTCTGG CAAACAGTAC CTCCATGATT TCTTTGGTAT CTTTGGGCGT 
CTCTCAGCCT GGTGCTTGCA GAATCCAGGT CACGTGGCAG AGATTTACCT TGTCCATCTT 
CATGCCAGTG TTTATGCTCT CTTTCATCGG CTTTATGGAA TGTATCCTTG CAATTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACTTGG AGACCTTTGA AGAGGTAGTC 
AAGCCAATGA TGGAACATGT GCGAATTCAT CCAGAATTAG TGACTGGATC TAAGGACCAT 
GAACTGGACC CACGAAGGTG GAAAAGACTA GAAACTCATG ATGTTGTGAT AGAATGTGCC 
AAAATCTCCC TGGACCCTGC AGAAGCCTCA TATGAAGATG GCTATTACTC TGCGTCTCGG 
AAATCCTGTA CAAACTTAAA ACATCATCAA ACTGACCCCA GTGCCAGCCA TTACATTGAC 
ACACAGAGCA GCTATGGGAC GTCTACCCCA TATTCCACTC CTCGGCTAAC ACTATCACAA 
CTGCCAGGGC AGCTACCTCA GATTCTGAGC CCGCAGTCAA TAAAGCTGTC AACTGAGCCA 
CAGCAGGTTA CCATCTGGAG TCCTTCTGCA GTTTGTGGTA TGACCACTCC ACCAACCTCC 
CCTGGAATTG TCCCATCAGA GTCATCCCAG TCAACATCAC AACCTTACAG CAAAGCTTTT 
GGCACATCTG CAGTGGGGAA GGGAACACCT TTGGGGACAC CAGCCACATC TCCTCCACCC 
TGCACTTCAG ATGACTTTGT GCATGTCTCA CTTCCTTCAG CTGCTGCCAC ACCTCCTAAA 
AAGGAGGACA AACCAGATTC TGGGAGGCCT TCACTGTACC GACAGCAAAA TGTCATAAGC 
AGCGATAAAT CATTGGACAC ATCTGGTAGT AAAAGTTCAG TAACCTTAAG TGATCTTCCG 
GAGTTTTTAG GCGGTTTGTC TTTTGAAGAT AGTGCTGAAA AGGACAGAGA AGAAGATGCA 
ATATCTAAAG AGATCTCTGA GATCACGACT GATGCTGAAC ACATGGTGCC TAGAGGAGGA 
TTTGACTCTC CATTTTATCA CACAAATGAA AATCTGTCAG GTTCTCAGAA GAAGACCCAG 
TCAGTAGTCT CTAGTGTGCA GGGACACAGT CTGACCTCTG AGCCTTTAAC GTCTTCTCTG 
GACAAGCCTG GGCCTGAGAG TGCACTGGAG ACACCCAAAC AAACATTTAC TCCCATAGAC 
AAGCCCTGCG GAGGCTCTGG TGAAAGCCCT GCTGGTAACA GGGAAGGAAC CTCTGGGGAG 
ACAAGTATTC TCACTCCCAG CCCTTGCAAA GCATCAGCAC AAGGAAGAGC AGTGTTTGGG 
AGTGGGCAGC CTCCCCTGTA TGAGCACCTT TTTGAGGTTG CGCTACCAAA GACTGCCTAC 
CTCTTTGTTG GCAAGAAGAC TGAGGAGCTG CTAAAGAAAG CCAAGGGAAC CCAGGATAAA 
GACTGCATGT CCTCTACTTC TCCTGTGGAA GTACTGGACA GACTGATACA GCAAGGGGCA 
GATGCACACA CTAAGGAACT GAACAAATTG TCTCTGCCAA GCAAATCTGC TGACTGGACT 
CACTTTGGAG GTTCTCCCCC TTCAGATGAG ATTCACACCC TGCGTAACCA ATTGCTGTTG 
CTGCACAACC AGTTGCTGTA TGAACGCTTC AAAAGACAGC AGCATGCCCT TCGGAACCGG 
CGGCTCTTGC GGAAAGTGAT TAAAGCCACA GCACTGGAGG AACATAATGC TGCCATGAAA 
GATCAGCTGA AATTACAGGA AAAAGAAATC CAGGCCTTGA AACTGAGTCT GCAGAAAGAA 
CAGGCCAGGT ACCACCAGTT TCAGGAGGAG CACGAAGGGA TAGTGGCTCA GCTTCACAGC 
CAGATCAGAC AGCTGCAACA TGACCGTGAG GAATTCTACA ACCAGAGCCA GGAATTGCAG 
ACCAAGCTGG AAGACTGCAG GAACATGATT GCAGATCTGA GGTTAGAATT GAAAAAGGCT 
AACAACAAAG TCTGTCACAC AGAACTGCTT CTTAGCCAAG TTTCTCAAAA GCTTTCCAAC 
AGTGAATCAG TGCAACAGCA GATGGAGTTC TTGAACAGAC AACTTCTGGT TCTTGGAGAG 
GTCAATGAGT TGTACCTGGA GCAGCTGCAG CACAAGCACA CAGACACTAC AAAGGAGGTT 
GAAATGTTGC ATGCTGCTTT TCGAAAGGAA CTGGAGAAAG CAAAATTGTG TGTTCAGCAG 
CAAAGCCAAA GGCTCGATGC TTCTCAGAAA CGGATAGCTG AACTGGAATC TCAGCTTTCT 
AAAAAGGACC ACCTCTTCTT GGAGCAAAAG AAATACCTGG AAGATGTCAA AATCAAAGCA 
AGAGGTCAGT TGCAAGCAGT AGAGAGTAGA TACAGGGCCC AGAAAAGGAT CACCCAGGCG 
TTTGAGCTGG AGATCTTGAA CCTGTTTGGC CGACTGGAGA ACAGCCCGCT GAAAAAACTT 
GAAGATGAAA AAACAGAAGC AGCTGAAGCA GCAGAAGAAA GGCTGGATTG TGGTAATGAA 
GGTTGTGCAG ATACTGTGGC AGGATACAGC GAAGAAACCA TTGATAGAAA TGGAGAAACC 
AAACCTCCCA GCACTCGAGG TAGCAGTAAA GGTGGCAGCA ACAGTGAGCT CTCCACCCCA 
GAAAAAAAAC AGAACCAGAG ATTCAGCAGT CACTGGGAGA CCTCCATGTT GCGGGAGCCC 
TCCACTACTG TCCCACTGAC TGTAGGTTCA CTCCCCAGCT CCAAGAGCTT CCTTGGTATG 
AAGTCACGAG AGTTGTTTCG CAACAAAAGT GAGAGCCAGT GTGATGAGCA TGGTGTAACC 
ATCAACAGCC TTTCTGATAC TCTAAAGACT GAACTATGTA AAGATCCAAG CATGGAGACG 
AAGGCTCCTC CAAGCCCCGA TAACCTTAGC CTCTCACCTA AGATCCAGGA AAGCAGTGTT 
GGACAGCTTC ATATCATGGA CTACAATGAA ACTCATCATG ACCACAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135246.1
CDS	178..3648
Translation	MAQQGNVGELLSMLDSPILGVLEDITAAFKDNLICDRGPMLVNSLVDYYLETNSQQALHILSTLQEPHDKHLLDKINEYMGKAATRLPTLSLLGHVIRRQPSWKHKLSQAPLLLSLLKCLKTDTDVVVLTTGVLVLITMLPMIPQSGKQYLHDFFGIFGRLSAWCLQNPGHVAEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPAEASYEDGYYSASRKSCTNLKHHQTDPSASHYIDTQSSYGTSTPYSTPRLTLSQLPGQLPQILSPQSIKLSTEPQQVTIWSPSAVCGMTTPPTSPGIVPSESSQSTSQPYSKAFGTSAVGKGTPLGTPATSPPPCTSDDFVHVSLPSAAATPPKKEDKPDSGRPSLYRQQNVISSDKSLDTSGSKSSVTLSDLPEFLGGLSFEDSAEKDREEDAISKEISEITTDAEHMVPRGGFDSPFYHTNENLSGSQKKTQSVVSSVQGHSLTSEPLTSSLDKPGPESALETPKQTFTPIDKPCGGSGESPAGNREGTSGETSILTPSPCKASAQGRAVFGSGQPPLYEHLFEVALPKTAYLFVGKKTEELLKKAKGTQDKDCMSSTSPVEVLDRLIQQGADAHTKELNKLSLPSKSADWTHFGGSPPSDEIHTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKATALEEHNAAMKDQLKLQEKEIQALKLSLQKEQARYHQFQEEHEGIVAQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIADLRLELKKANNKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQHKHTDTTKEVEMLHAAFRKELEKAKLCVQQQSQRLDASQKRIAELESQLSKKDHLFLEQKKYLEDVKIKARGQLQAVESRYRAQKRITQAFELEILNLFGRLENSPLKKLEDEKTEAAEAAEERLDCGNEGCADTVAGYSEETIDRNGETKPPSTRGSSKGGSNSELSTPEKKQNQRFSSHWETSMLREPSTTVPLTVGSLPSSKSFLGMKSRELFRNKSESQCDEHGVTINSLSDTLKTELCKDPSMETKAPPSPDNLSLSPKIQESSVGQLHIMDYNETHHDHS

Target ORF information:

RefSeq Version	XM_015279760.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279760.2

ORF Insert Sequence:

ATGGCACAGC AGGGGAATGT TGGTGAGCTC CTCTCAATGC TGGATTCTCC AATTCTGGGT 
GTCCTGGAAG ACATAACAGC TGCATTCAAA GATAATCTCA TTTGTGACCG TGGACCTATG 
CTTGTGAACA GCCTGGTAGA CTATTACTTG GAAACCAATT CTCAGCAGGC ACTGCATATA 
CTGTCCACAT TGCAGGAGCC TCATGACAAG CACCTTCTGG ACAAAATTAA TGAATATATG 
GGCAAAGCTG CTACTCGTTT ACCCACTCTC TCGCTGCTGG GACATGTGAT AAGGCGACAA 
CCATCGTGGA AACATAAGCT TTCTCAAGCA CCTCTCCTAC TTTCATTACT TAAATGTCTC 
AAGACTGACA CAGATGTAGT AGTACTCACC ACAGGTGTCC TAGTGCTAAT AACCATGTTG 
CCAATGATTC CTCAGTCTGG CAAACAGTAC CTCCATGATT TCTTTGGTAT CTTTGGGCGT 
CTCTCAGCCT GGTGCTTGCA GAATCCAGGT CACGTGGCAG AGATTTACCT TGTCCATCTT 
CATGCCAGTG TTTATGCTCT CTTTCATCGG CTTTATGGAA TGTATCCTTG CAATTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACTTGG AGACCTTTGA AGAGGTAGTC 
AAGCCAATGA TGGAACATGT GCGAATTCAT CCAGAATTAG TGACTGGATC TAAGGACCAT 
GAACTGGACC CACGAAGGTG GAAAAGACTA GAAACTCATG ATGTTGTGAT AGAATGTGCC 
AAAATCTCCC TGGACCCTGC AGAAGCCTCA TATGAAGATG GCTATTACTC TGCGTCTCGG 
AAATCCTGTA CAAACTTAAA ACATCATCAA ACTGACCCCA GTGCCAGCCA TTACATTGAC 
ACACAGAGCA GCTATGGGAC GTCTACCCCA TATTCCACTC CTCGGCTAAC ACTATCACAA 
CTGCCAGGGC AGCTACCTCA GATTCTGAGC CCGCAGTCAA TAAAGCTGTC AACTGAGCCA 
CAGCAGGTTA CCATCTGGAG TCCTTCTGCA GTTTGTGGTA TGACCACTCC ACCAACCTCC 
CCTGGAATTG TCCCATCAGA GTCATCCCAG TCAACATCAC AACCTTACAG CAAAGCTTTT 
GGCACATCTG CAGTGGGGAA GGGAACACCT TTGGGGACAC CAGCCACATC TCCTCCACCC 
TGCACTTCAG ATGACTTTGT GCATGTCTCA CTTCCTTCAG CTGCTGCCAC ACCTCCTAAA 
AAGGAGGACA AACCAGATTC TGGGAGGCCT TCACTGTACC GACAGCAAAA TGTCATAAGC 
AGCGATAAAT CATTGGACAC ATCTGGTAGT AAAAGTTCAG TAACCTTAAG TGATCTTCCG 
GAGTTTTTAG GCGGTTTGTC TTTTGAAGAT AGTGCTGAAA AGGACAGAGA AGAAGATGCA 
ATATCTAAAG AGATCTCTGA GATCACGACT GATGCTGAAC ACATGGTGCC TAGAGGAGGA 
TTTGACTCTC CATTTTATCA CACAAATGAA AATCTGTCAG GTTCTCAGAA GAAGACCCAG 
TCAGTAGTCT CTAGTGTGCA GGGACACAGT CTGACCTCTG AGCCTTTAAC GTCTTCTCTG 
GACAAGCCTG GGCCTGAGAG TGCACTGGAG ACACCCAAAC AAACATTTAC TCCCATAGAC 
AAGCCCTGCG GAGGCTCTGG TGAAAGCCCT GCTGGTAACA GGGAAGGAAC CTCTGGGGAG 
ACAAGTATTC TCACTCCCAG CCCTTGCAAA GCATCAGCAC AAGGAAGAGC AGTGTTTGGG 
AGTGGGCAGC CTCCCCTGTA TGAGCACCTT TTTGAGGTTG CGCTACCAAA GACTGCCTAC 
CTCTTTGTTG GCAAGAAGAC TGAGGAGCTG CTAAAGAAAG CCAAGGGAAC CCAGGATAAA 
GACTGCATGT CCTCTACTTC TCCTGTGGAA GTACTGGACA GACTGATACA GCAAGGGGCA 
GATGCACACA CTAAGGAACT GAACAAATTG TCTCTGCCAA GCAAATCTGC TGACTGGACT 
CACTTTGGAG GTTCTCCCCC TTCAGATGAG ATTCACACCC TGCGTAACCA ATTGCTGTTG 
CTGCACAACC AGTTGCTGTA TGAACGCTTC AAAAGACAGC AGCATGCCCT TCGGAACCGG 
CGGCTCTTGC GGAAAGTGAT TAAAGCCACA GCACTGGAGG AACATAATGC TGCCATGAAA 
GATCAGCTGA AATTACAGGA AAAAGAAATC CAGGCCTTGA AACTGAGTCT GCAGAAAGAA 
CAGGCCAGGT ACCACCAGTT TCAGGAGGAG CACGAAGGGA TAGTGGCTCA GCTTCACAGC 
CAGATCAGAC AGCTGCAACA TGACCGTGAG GAATTCTACA ACCAGAGCCA GGAATTGCAG 
ACCAAGCTGG AAGACTGCAG GAACATGATT GCAGATCTGA GGTTAGAATT GAAAAAGGCT 
AACAACAAAG TCTGTCACAC AGAACTGCTT CTTAGCCAAG TTTCTCAAAA GCTTTCCAAC 
AGTGAATCAG TGCAACAGCA GATGGAGTTC TTGAACAGAC AACTTCTGGT TCTTGGAGAG 
GTCAATGAGT TGTACCTGGA GCAGCTGCAG CACAAGCACA CAGACACTAC AAAGGAGGTT 
GAAATGTTGC ATGCTGCTTT TCGAAAGGAA CTGGAGAAAG CAAAATTGTG TGTTCAGCAG 
CAAAGCCAAA GGCTCGATGC TTCTCAGAAA CGGATAGCTG AACTGGAATC TCAGCTTTCT 
AAAAAGGACC ACCTCTTCTT GGAGCAAAAG AAATACCTGG AAGATGTCAA AATCAAAGCA 
AGAGGTCAGT TGCAAGCAGT AGAGAGTAGA TACAGGGCCC AGAAAAGGAT CACCCAGGCG 
TTTGAGCTGG AGATCTTGAA CCTGTTTGGC CGACTGGAGA ACAGCCCGCT GAAAAAACTT 
GAAGATGAAA AAACAGAAGC AGCTGAAGCA GCAGAAGAAA GGCTGGATTG TGGTAATGAA 
GGTTGTGCAG ATACTGTGGC AGGATACAGC GAAGAAACCA TTGATAGAAA TGGAGAAACC 
AAACCTCCCA GCACTCGAGG TAGCAGTAAA GGTGGCAGCA ACAGTGAGCT CTCCACCCCA 
GAAAAAAAAC AGAACCAGAG ATTCAGCAGT CACTGGGAGA CCTCCATGTT GCGGGAGCCC 
TCCACTACTG TCCCACTGAC TGTAGGTTCA CTCCCCAGCT CCAAGAGCTT CCTTGGTATG 
AAGTCACGAG AGTTGTTTCG CAACAAAAGT GAGAGCCAGT GTGATGAGCA TGGTGTAACC 
ATCAACAGCC TTTCTGATAC TCTAAAGACT GAACTATGTA AAGATCCAAG CATGGAGACG 
AAGGCTCCTC CAAGCCCCGA TAACCTTAGC CTCTCACCTA AGATCCAGGA AAGCAGTGTT 
GGACAGCTTC ATATCATGGA CTACAATGAA ACTCATCATG ACCACAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_004945922.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004945979.1 Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA.

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CloneID	OGa43673
Clone ID Related Accession (Same CDS sequence)	XM_004945922.2 , XM_004945922.3
Accession Version	XM_004945922.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	hamartin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004945922.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGC AGGGGAATGT TGGTGAGCTC CTCTCAATGC TGGATTCTCC AATTCTGGGT 
GTCCTGGAAG ACATAACAGC TGCATTCAAA GATAATCTCA TTTGTGACCG TGGACCTATG 
CTTGTGAACA GCCTGGTAGA CTATTACTTG GAAACCAATT CTCAGCAGGC ACTGCATATA 
CTGTCCACAT TGCAGGAGCC TCATGACAAG CACCTTCTGG ACAAAATTAA TGAATATATG 
GGCAAAGCTG CTACTCGTTT ACCCACTCTC TCGCTGCTGG GACATGTGAT AAGGCGACAA 
CCATCGTGGA AACATAAGCT TTCTCAAGCA CCTCTCCTAC TTTCATTACT TAAATGTCTC 
AAGACTGACA CAGATGTAGT AGTACTCACC ACAGGTGTCC TAGTGCTAAT AACCATGTTG 
CCAATGATTC CTCAGTCTGG CAAACAGTAC CTCCATGATT TCTTTGGTAT CTTTGGGCGT 
CTCTCAGCCT GGTGCTTGCA GAATCCAGGT CACGTGGCAG AGATTTACCT TGTCCATCTT 
CATGCCAGTG TTTATGCTCT CTTTCATCGG CTTTATGGAA TGTATCCTTG CAATTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACTTGG AGACCTTTGA AGAGGTAGTC 
AAGCCAATGA TGGAACATGT GCGAATTCAT CCAGAATTAG TGACTGGATC TAAGGACCAT 
GAACTGGACC CACGAAGGTG GAAAAGACTA GAAACTCATG ATGTTGTGAT AGAATGTGCC 
AAAATCTCCC TGGACCCTGC AGAAGCCTCA TATGAAGATG GCTATTACTC TGCGTCTCGG 
AAATCCTGTA CAAACTTAAA ACATCATCAA ACTGACCCCA GTGCCAGCCA TTACATTGAC 
ACACAGAGCA GCTATGGGAC GTCTACCCCA TATTCCACTC CTCGGCTAAC ACTATCACAA 
CTGCCAGGGC AGCTACCTCA GATTCTGAGC CCGCAGTCAA TAAAGCTGTC AACTGAGCCA 
CAGCAGGTTA CCATCTGGAG TCCTTCTGCA GTTTGTGGTA TGACCACTCC ACCAACCTCC 
CCTGGAATTG TCCCATCAGA GTCATCCCAG TCAACATCAC AACCTTACAG CAAAGCTTTT 
GGCACATCTG CAGTGGGGAA GGGAACACCT TTGGGGACAC CAGCCACATC TCCTCCACCC 
TGCACTTCAG ATGACTTTGT GCATGTCTCA CTTCCTTCAG CTGCTGCCAC ACCTCCTAAA 
AAGGAGGACA AACCAGATTC TGGGAGGCCT TCACTGTACC GACAGCAAAA TGTCATAAGC 
AGCGATAAAT CATTGGACAC ATCTGGTAGT AAAAGTTCAG TAACCTTAAG TGATCTTCCG 
GAGTTTTTAG GCGGTTTGTC TTTTGAAGAT AGTGCTGAAA AGGACAGAGA AGAAGATGCA 
ATATCTAAAG AGATCTCTGA GATCACGACT GATGCTGAAC ACATGGTGCC TAGAGGAGGA 
TTTGACTCTC CATTTTATCA CACAAATGAA AATCTGTCAG GTTCTCAGAA GAAGACCCAG 
TCAGTAGTCT CTAGTGTGCA GGGACACAGT CTGACCTCTG AGCCTTTAAC GTCTTCTCTG 
GACAAGCCTG GGCCTGAGAG TGCACTGGAG ACACCCAAAC AAACATTTAC TCCCATAGAC 
AAGCCCTGCG GAGGCTCTGG TGAAAGCCCT GCTGGTAACA GGGAAGGAAC CTCTGGGGAG 
ACAAGTATTC TCACTCCCAG CCCTTGCAAA GCATCAGCAC AAGGAAGAGC AGTGTTTGGG 
AGTGGGCAGC CTCCCCTGTA TGAGCACCTT TTTGAGGTTG CGCTACCAAA GACTGCCTAC 
CTCTTTGTTG GCAAGAAGAC TGAGGAGCTG CTAAAGAAAG CCAAGGGAAC CCAGGATAAA 
GACTGCATGT CCTCTACTTC TCCTGTGGAA GTACTGGACA GACTGATACA GCAAGGGGCA 
GATGCACACA CTAAGGAACT GAACAAATTG TCTCTGCCAA GCAAATCTGC TGACTGGACT 
CACTTTGGAG GTTCTCCCCC TTCAGATGAG ATTCACACCC TGCGTAACCA ATTGCTGTTG 
CTGCACAACC AGTTGCTGTA TGAACGCTTC AAAAGACAGC AGCATGCCCT TCGGAACCGG 
CGGCTCTTGC GGAAAGTGAT TAAAGCCACA GCACTGGAGG AACATAATGC TGCCATGAAA 
GATCAGCTGA AATTACAGGA AAAAGAAATC CAGGCCTTGA AACTGAGTCT GCAGAAAGAA 
CAGGCCAGGT ACCACCAGTT TCAGGAGGAG CACGAAGGGA TAGTGGCTCA GCTTCACAGC 
CAGATCAGAC AGCTGCAACA TGACCGTGAG GAATTCTACA ACCAGAGCCA GGAATTGCAG 
ACCAAGCTGG AAGACTGCAG GAACATGATT GCAGATCTGA GGTTAGAATT GAAAAAGGCT 
AACAACAAAG TCTGTCACAC AGAACTGCTT CTTAGCCAAG TTTCTCAAAA GCTTTCCAAC 
AGTGAATCAG TGCAACAGCA GATGGAGTTC TTGAACAGAC AACTTCTGGT TCTTGGAGAG 
GTCAATGAGT TGTACCTGGA GCAGCTGCAG CACAAGCACA CAGACACTAC AAAGGTTGAA 
ATGTTGCATG CTGCTTTTCG AAAGGAACTG GAGAAAGCAA AATTGTGTGT TCAGCAGCAA 
AGCCAAAGGC TCGATGCTTC TCAGAAACGG ATAGCTGAAC TGGAATCTCA GCTTTCTAAA 
AAGGACCACC TCTTCTTGGA GCAAAAGAAA TACCTGGAAG ATGTCAAAAT CAAAGCAAGA 
GGTCAGTTGC AAGCAGTAGA GAGTAGATAC AGGGCCCAGA AAAGGATCAC CCAGGCGTTT 
GAGCTGGAGA TCTTGAACCT GTTTGGCCGA CTGGAGAACA GCCCGCTGAA AAAACTTGAA 
GATGAAAAAA CAGAAGCAGC TGAAGCAGCA GAAGAAAGGC TGGATTGTGG TAATGAAGGT 
TGTGCAGATA CTGTGGCAGG ATACAGCGAA GAAACCATTG ATAGAAATGG AGAAACCAAA 
CCTCCCAGCA CTCGAGGTAG CAGTAAAGGT GGCAGCAACA GTGAGCTCTC CACCCCAGAA 
AAAAAACAGA ACCAGAGATT CAGCAGTCAC TGGGAGACCT CCATGTTGCG GGAGCCCTCC 
ACTACTGTCC CACTGACTGT AGGTTCACTC CCCAGCTCCA AGAGCTTCCT TGGTATGAAG 
TCACGAGAGT TGTTTCGCAA CAAAAGTGAG AGCCAGTGTG ATGAGCATGG TGTAACCATC 
AACAGCCTTT CTGATACTCT AAAGACTGAA CTATGTAAAG ATCCAAGCAT GGAGACGAAG 
GCTCCTCCAA GCCCCGATAA CCTTAGCCTC TCACCTAAGA TCCAGGAAAG CAGTGTTGGA 
CAGCTTCATA TCATGGACTA CAATGAAACT CATCATGACC ACAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004945979.1
CDS	219..3686
Translation	MAQQGNVGELLSMLDSPILGVLEDITAAFKDNLICDRGPMLVNSLVDYYLETNSQQALHILSTLQEPHDKHLLDKINEYMGKAATRLPTLSLLGHVIRRQPSWKHKLSQAPLLLSLLKCLKTDTDVVVLTTGVLVLITMLPMIPQSGKQYLHDFFGIFGRLSAWCLQNPGHVAEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPAEASYEDGYYSASRKSCTNLKHHQTDPSASHYIDTQSSYGTSTPYSTPRLTLSQLPGQLPQILSPQSIKLSTEPQQVTIWSPSAVCGMTTPPTSPGIVPSESSQSTSQPYSKAFGTSAVGKGTPLGTPATSPPPCTSDDFVHVSLPSAAATPPKKEDKPDSGRPSLYRQQNVISSDKSLDTSGSKSSVTLSDLPEFLGGLSFEDSAEKDREEDAISKEISEITTDAEHMVPRGGFDSPFYHTNENLSGSQKKTQSVVSSVQGHSLTSEPLTSSLDKPGPESALETPKQTFTPIDKPCGGSGESPAGNREGTSGETSILTPSPCKASAQGRAVFGSGQPPLYEHLFEVALPKTAYLFVGKKTEELLKKAKGTQDKDCMSSTSPVEVLDRLIQQGADAHTKELNKLSLPSKSADWTHFGGSPPSDEIHTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKATALEEHNAAMKDQLKLQEKEIQALKLSLQKEQARYHQFQEEHEGIVAQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIADLRLELKKANNKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQHKHTDTTKVEMLHAAFRKELEKAKLCVQQQSQRLDASQKRIAELESQLSKKDHLFLEQKKYLEDVKIKARGQLQAVESRYRAQKRITQAFELEILNLFGRLENSPLKKLEDEKTEAAEAAEERLDCGNEGCADTVAGYSEETIDRNGETKPPSTRGSSKGGSNSELSTPEKKQNQRFSSHWETSMLREPSTTVPLTVGSLPSSKSFLGMKSRELFRNKSESQCDEHGVTINSLSDTLKTELCKDPSMETKAPPSPDNLSLSPKIQESSVGQLHIMDYNETHHDHS

Target ORF information:

RefSeq Version	XM_004945922.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004945922.2

ORF Insert Sequence:

ATGGCACAGC AGGGGAATGT TGGTGAGCTC CTCTCAATGC TGGATTCTCC AATTCTGGGT 
GTCCTGGAAG ACATAACAGC TGCATTCAAA GATAATCTCA TTTGTGACCG TGGACCTATG 
CTTGTGAACA GCCTGGTAGA CTATTACTTG GAAACCAATT CTCAGCAGGC ACTGCATATA 
CTGTCCACAT TGCAGGAGCC TCATGACAAG CACCTTCTGG ACAAAATTAA TGAATATATG 
GGCAAAGCTG CTACTCGTTT ACCCACTCTC TCGCTGCTGG GACATGTGAT AAGGCGACAA 
CCATCGTGGA AACATAAGCT TTCTCAAGCA CCTCTCCTAC TTTCATTACT TAAATGTCTC 
AAGACTGACA CAGATGTAGT AGTACTCACC ACAGGTGTCC TAGTGCTAAT AACCATGTTG 
CCAATGATTC CTCAGTCTGG CAAACAGTAC CTCCATGATT TCTTTGGTAT CTTTGGGCGT 
CTCTCAGCCT GGTGCTTGCA GAATCCAGGT CACGTGGCAG AGATTTACCT TGTCCATCTT 
CATGCCAGTG TTTATGCTCT CTTTCATCGG CTTTATGGAA TGTATCCTTG CAATTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACTTGG AGACCTTTGA AGAGGTAGTC 
AAGCCAATGA TGGAACATGT GCGAATTCAT CCAGAATTAG TGACTGGATC TAAGGACCAT 
GAACTGGACC CACGAAGGTG GAAAAGACTA GAAACTCATG ATGTTGTGAT AGAATGTGCC 
AAAATCTCCC TGGACCCTGC AGAAGCCTCA TATGAAGATG GCTATTACTC TGCGTCTCGG 
AAATCCTGTA CAAACTTAAA ACATCATCAA ACTGACCCCA GTGCCAGCCA TTACATTGAC 
ACACAGAGCA GCTATGGGAC GTCTACCCCA TATTCCACTC CTCGGCTAAC ACTATCACAA 
CTGCCAGGGC AGCTACCTCA GATTCTGAGC CCGCAGTCAA TAAAGCTGTC AACTGAGCCA 
CAGCAGGTTA CCATCTGGAG TCCTTCTGCA GTTTGTGGTA TGACCACTCC ACCAACCTCC 
CCTGGAATTG TCCCATCAGA GTCATCCCAG TCAACATCAC AACCTTACAG CAAAGCTTTT 
GGCACATCTG CAGTGGGGAA GGGAACACCT TTGGGGACAC CAGCCACATC TCCTCCACCC 
TGCACTTCAG ATGACTTTGT GCATGTCTCA CTTCCTTCAG CTGCTGCCAC ACCTCCTAAA 
AAGGAGGACA AACCAGATTC TGGGAGGCCT TCACTGTACC GACAGCAAAA TGTCATAAGC 
AGCGATAAAT CATTGGACAC ATCTGGTAGT AAAAGTTCAG TAACCTTAAG TGATCTTCCG 
GAGTTTTTAG GCGGTTTGTC TTTTGAAGAT AGTGCTGAAA AGGACAGAGA AGAAGATGCA 
ATATCTAAAG AGATCTCTGA GATCACGACT GATGCTGAAC ACATGGTGCC TAGAGGAGGA 
TTTGACTCTC CATTTTATCA CACAAATGAA AATCTGTCAG GTTCTCAGAA GAAGACCCAG 
TCAGTAGTCT CTAGTGTGCA GGGACACAGT CTGACCTCTG AGCCTTTAAC GTCTTCTCTG 
GACAAGCCTG GGCCTGAGAG TGCACTGGAG ACACCCAAAC AAACATTTAC TCCCATAGAC 
AAGCCCTGCG GAGGCTCTGG TGAAAGCCCT GCTGGTAACA GGGAAGGAAC CTCTGGGGAG 
ACAAGTATTC TCACTCCCAG CCCTTGCAAA GCATCAGCAC AAGGAAGAGC AGTGTTTGGG 
AGTGGGCAGC CTCCCCTGTA TGAGCACCTT TTTGAGGTTG CGCTACCAAA GACTGCCTAC 
CTCTTTGTTG GCAAGAAGAC TGAGGAGCTG CTAAAGAAAG CCAAGGGAAC CCAGGATAAA 
GACTGCATGT CCTCTACTTC TCCTGTGGAA GTACTGGACA GACTGATACA GCAAGGGGCA 
GATGCACACA CTAAGGAACT GAACAAATTG TCTCTGCCAA GCAAATCTGC TGACTGGACT 
CACTTTGGAG GTTCTCCCCC TTCAGATGAG ATTCACACCC TGCGTAACCA ATTGCTGTTG 
CTGCACAACC AGTTGCTGTA TGAACGCTTC AAAAGACAGC AGCATGCCCT TCGGAACCGG 
CGGCTCTTGC GGAAAGTGAT TAAAGCCACA GCACTGGAGG AACATAATGC TGCCATGAAA 
GATCAGCTGA AATTACAGGA AAAAGAAATC CAGGCCTTGA AACTGAGTCT GCAGAAAGAA 
CAGGCCAGGT ACCACCAGTT TCAGGAGGAG CACGAAGGGA TAGTGGCTCA GCTTCACAGC 
CAGATCAGAC AGCTGCAACA TGACCGTGAG GAATTCTACA ACCAGAGCCA GGAATTGCAG 
ACCAAGCTGG AAGACTGCAG GAACATGATT GCAGATCTGA GGTTAGAATT GAAAAAGGCT 
AACAACAAAG TCTGTCACAC AGAACTGCTT CTTAGCCAAG TTTCTCAAAA GCTTTCCAAC 
AGTGAATCAG TGCAACAGCA GATGGAGTTC TTGAACAGAC AACTTCTGGT TCTTGGAGAG 
GTCAATGAGT TGTACCTGGA GCAGCTGCAG CACAAGCACA CAGACACTAC AAAGGTTGAA 
ATGTTGCATG CTGCTTTTCG AAAGGAACTG GAGAAAGCAA AATTGTGTGT TCAGCAGCAA 
AGCCAAAGGC TCGATGCTTC TCAGAAACGG ATAGCTGAAC TGGAATCTCA GCTTTCTAAA 
AAGGACCACC TCTTCTTGGA GCAAAAGAAA TACCTGGAAG ATGTCAAAAT CAAAGCAAGA 
GGTCAGTTGC AAGCAGTAGA GAGTAGATAC AGGGCCCAGA AAAGGATCAC CCAGGCGTTT 
GAGCTGGAGA TCTTGAACCT GTTTGGCCGA CTGGAGAACA GCCCGCTGAA AAAACTTGAA 
GATGAAAAAA CAGAAGCAGC TGAAGCAGCA GAAGAAAGGC TGGATTGTGG TAATGAAGGT 
TGTGCAGATA CTGTGGCAGG ATACAGCGAA GAAACCATTG ATAGAAATGG AGAAACCAAA 
CCTCCCAGCA CTCGAGGTAG CAGTAAAGGT GGCAGCAACA GTGAGCTCTC CACCCCAGAA 
AAAAAACAGA ACCAGAGATT CAGCAGTCAC TGGGAGACCT CCATGTTGCG GGAGCCCTCC 
ACTACTGTCC CACTGACTGT AGGTTCACTC CCCAGCTCCA AGAGCTTCCT TGGTATGAAG 
TCACGAGAGT TGTTTCGCAA CAAAAGTGAG AGCCAGTGTG ATGAGCATGG TGTAACCATC 
AACAGCCTTT CTGATACTCT AAAGACTGAA CTATGTAAAG ATCCAAGCAT GGAGACGAAG 
GCTCCTCCAA GCCCCGATAA CCTTAGCCTC TCACCTAAGA TCCAGGAAAG CAGTGTTGGA 
CAGCTTCATA TCATGGACTA CAATGAAACT CATCATGACC ACAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_004945922.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004945979.1 Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA.

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CloneID	OGa43673
Clone ID Related Accession (Same CDS sequence)	XM_004945922.2 , XM_004945922.3
Accession Version	XM_004945922.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	hamartin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004945922.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGC AGGGGAATGT TGGTGAGCTC CTCTCAATGC TGGATTCTCC AATTCTGGGT 
GTCCTGGAAG ACATAACAGC TGCATTCAAA GATAATCTCA TTTGTGACCG TGGACCTATG 
CTTGTGAACA GCCTGGTAGA CTATTACTTG GAAACCAATT CTCAGCAGGC ACTGCATATA 
CTGTCCACAT TGCAGGAGCC TCATGACAAG CACCTTCTGG ACAAAATTAA TGAATATATG 
GGCAAAGCTG CTACTCGTTT ACCCACTCTC TCGCTGCTGG GACATGTGAT AAGGCGACAA 
CCATCGTGGA AACATAAGCT TTCTCAAGCA CCTCTCCTAC TTTCATTACT TAAATGTCTC 
AAGACTGACA CAGATGTAGT AGTACTCACC ACAGGTGTCC TAGTGCTAAT AACCATGTTG 
CCAATGATTC CTCAGTCTGG CAAACAGTAC CTCCATGATT TCTTTGGTAT CTTTGGGCGT 
CTCTCAGCCT GGTGCTTGCA GAATCCAGGT CACGTGGCAG AGATTTACCT TGTCCATCTT 
CATGCCAGTG TTTATGCTCT CTTTCATCGG CTTTATGGAA TGTATCCTTG CAATTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACTTGG AGACCTTTGA AGAGGTAGTC 
AAGCCAATGA TGGAACATGT GCGAATTCAT CCAGAATTAG TGACTGGATC TAAGGACCAT 
GAACTGGACC CACGAAGGTG GAAAAGACTA GAAACTCATG ATGTTGTGAT AGAATGTGCC 
AAAATCTCCC TGGACCCTGC AGAAGCCTCA TATGAAGATG GCTATTACTC TGCGTCTCGG 
AAATCCTGTA CAAACTTAAA ACATCATCAA ACTGACCCCA GTGCCAGCCA TTACATTGAC 
ACACAGAGCA GCTATGGGAC GTCTACCCCA TATTCCACTC CTCGGCTAAC ACTATCACAA 
CTGCCAGGGC AGCTACCTCA GATTCTGAGC CCGCAGTCAA TAAAGCTGTC AACTGAGCCA 
CAGCAGGTTA CCATCTGGAG TCCTTCTGCA GTTTGTGGTA TGACCACTCC ACCAACCTCC 
CCTGGAATTG TCCCATCAGA GTCATCCCAG TCAACATCAC AACCTTACAG CAAAGCTTTT 
GGCACATCTG CAGTGGGGAA GGGAACACCT TTGGGGACAC CAGCCACATC TCCTCCACCC 
TGCACTTCAG ATGACTTTGT GCATGTCTCA CTTCCTTCAG CTGCTGCCAC ACCTCCTAAA 
AAGGAGGACA AACCAGATTC TGGGAGGCCT TCACTGTACC GACAGCAAAA TGTCATAAGC 
AGCGATAAAT CATTGGACAC ATCTGGTAGT AAAAGTTCAG TAACCTTAAG TGATCTTCCG 
GAGTTTTTAG GCGGTTTGTC TTTTGAAGAT AGTGCTGAAA AGGACAGAGA AGAAGATGCA 
ATATCTAAAG AGATCTCTGA GATCACGACT GATGCTGAAC ACATGGTGCC TAGAGGAGGA 
TTTGACTCTC CATTTTATCA CACAAATGAA AATCTGTCAG GTTCTCAGAA GAAGACCCAG 
TCAGTAGTCT CTAGTGTGCA GGGACACAGT CTGACCTCTG AGCCTTTAAC GTCTTCTCTG 
GACAAGCCTG GGCCTGAGAG TGCACTGGAG ACACCCAAAC AAACATTTAC TCCCATAGAC 
AAGCCCTGCG GAGGCTCTGG TGAAAGCCCT GCTGGTAACA GGGAAGGAAC CTCTGGGGAG 
ACAAGTATTC TCACTCCCAG CCCTTGCAAA GCATCAGCAC AAGGAAGAGC AGTGTTTGGG 
AGTGGGCAGC CTCCCCTGTA TGAGCACCTT TTTGAGGTTG CGCTACCAAA GACTGCCTAC 
CTCTTTGTTG GCAAGAAGAC TGAGGAGCTG CTAAAGAAAG CCAAGGGAAC CCAGGATAAA 
GACTGCATGT CCTCTACTTC TCCTGTGGAA GTACTGGACA GACTGATACA GCAAGGGGCA 
GATGCACACA CTAAGGAACT GAACAAATTG TCTCTGCCAA GCAAATCTGC TGACTGGACT 
CACTTTGGAG GTTCTCCCCC TTCAGATGAG ATTCACACCC TGCGTAACCA ATTGCTGTTG 
CTGCACAACC AGTTGCTGTA TGAACGCTTC AAAAGACAGC AGCATGCCCT TCGGAACCGG 
CGGCTCTTGC GGAAAGTGAT TAAAGCCACA GCACTGGAGG AACATAATGC TGCCATGAAA 
GATCAGCTGA AATTACAGGA AAAAGAAATC CAGGCCTTGA AACTGAGTCT GCAGAAAGAA 
CAGGCCAGGT ACCACCAGTT TCAGGAGGAG CACGAAGGGA TAGTGGCTCA GCTTCACAGC 
CAGATCAGAC AGCTGCAACA TGACCGTGAG GAATTCTACA ACCAGAGCCA GGAATTGCAG 
ACCAAGCTGG AAGACTGCAG GAACATGATT GCAGATCTGA GGTTAGAATT GAAAAAGGCT 
AACAACAAAG TCTGTCACAC AGAACTGCTT CTTAGCCAAG TTTCTCAAAA GCTTTCCAAC 
AGTGAATCAG TGCAACAGCA GATGGAGTTC TTGAACAGAC AACTTCTGGT TCTTGGAGAG 
GTCAATGAGT TGTACCTGGA GCAGCTGCAG CACAAGCACA CAGACACTAC AAAGGTTGAA 
ATGTTGCATG CTGCTTTTCG AAAGGAACTG GAGAAAGCAA AATTGTGTGT TCAGCAGCAA 
AGCCAAAGGC TCGATGCTTC TCAGAAACGG ATAGCTGAAC TGGAATCTCA GCTTTCTAAA 
AAGGACCACC TCTTCTTGGA GCAAAAGAAA TACCTGGAAG ATGTCAAAAT CAAAGCAAGA 
GGTCAGTTGC AAGCAGTAGA GAGTAGATAC AGGGCCCAGA AAAGGATCAC CCAGGCGTTT 
GAGCTGGAGA TCTTGAACCT GTTTGGCCGA CTGGAGAACA GCCCGCTGAA AAAACTTGAA 
GATGAAAAAA CAGAAGCAGC TGAAGCAGCA GAAGAAAGGC TGGATTGTGG TAATGAAGGT 
TGTGCAGATA CTGTGGCAGG ATACAGCGAA GAAACCATTG ATAGAAATGG AGAAACCAAA 
CCTCCCAGCA CTCGAGGTAG CAGTAAAGGT GGCAGCAACA GTGAGCTCTC CACCCCAGAA 
AAAAAACAGA ACCAGAGATT CAGCAGTCAC TGGGAGACCT CCATGTTGCG GGAGCCCTCC 
ACTACTGTCC CACTGACTGT AGGTTCACTC CCCAGCTCCA AGAGCTTCCT TGGTATGAAG 
TCACGAGAGT TGTTTCGCAA CAAAAGTGAG AGCCAGTGTG ATGAGCATGG TGTAACCATC 
AACAGCCTTT CTGATACTCT AAAGACTGAA CTATGTAAAG ATCCAAGCAT GGAGACGAAG 
GCTCCTCCAA GCCCCGATAA CCTTAGCCTC TCACCTAAGA TCCAGGAAAG CAGTGTTGGA 
CAGCTTCATA TCATGGACTA CAATGAAACT CATCATGACC ACAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004945979.1
CDS	224..3691
Translation	MAQQGNVGELLSMLDSPILGVLEDITAAFKDNLICDRGPMLVNSLVDYYLETNSQQALHILSTLQEPHDKHLLDKINEYMGKAATRLPTLSLLGHVIRRQPSWKHKLSQAPLLLSLLKCLKTDTDVVVLTTGVLVLITMLPMIPQSGKQYLHDFFGIFGRLSAWCLQNPGHVAEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPAEASYEDGYYSASRKSCTNLKHHQTDPSASHYIDTQSSYGTSTPYSTPRLTLSQLPGQLPQILSPQSIKLSTEPQQVTIWSPSAVCGMTTPPTSPGIVPSESSQSTSQPYSKAFGTSAVGKGTPLGTPATSPPPCTSDDFVHVSLPSAAATPPKKEDKPDSGRPSLYRQQNVISSDKSLDTSGSKSSVTLSDLPEFLGGLSFEDSAEKDREEDAISKEISEITTDAEHMVPRGGFDSPFYHTNENLSGSQKKTQSVVSSVQGHSLTSEPLTSSLDKPGPESALETPKQTFTPIDKPCGGSGESPAGNREGTSGETSILTPSPCKASAQGRAVFGSGQPPLYEHLFEVALPKTAYLFVGKKTEELLKKAKGTQDKDCMSSTSPVEVLDRLIQQGADAHTKELNKLSLPSKSADWTHFGGSPPSDEIHTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKATALEEHNAAMKDQLKLQEKEIQALKLSLQKEQARYHQFQEEHEGIVAQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIADLRLELKKANNKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQHKHTDTTKVEMLHAAFRKELEKAKLCVQQQSQRLDASQKRIAELESQLSKKDHLFLEQKKYLEDVKIKARGQLQAVESRYRAQKRITQAFELEILNLFGRLENSPLKKLEDEKTEAAEAAEERLDCGNEGCADTVAGYSEETIDRNGETKPPSTRGSSKGGSNSELSTPEKKQNQRFSSHWETSMLREPSTTVPLTVGSLPSSKSFLGMKSRELFRNKSESQCDEHGVTINSLSDTLKTELCKDPSMETKAPPSPDNLSLSPKIQESSVGQLHIMDYNETHHDHS

Target ORF information:

RefSeq Version	XM_004945922.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004945922.3

ORF Insert Sequence:

ATGGCACAGC AGGGGAATGT TGGTGAGCTC CTCTCAATGC TGGATTCTCC AATTCTGGGT 
GTCCTGGAAG ACATAACAGC TGCATTCAAA GATAATCTCA TTTGTGACCG TGGACCTATG 
CTTGTGAACA GCCTGGTAGA CTATTACTTG GAAACCAATT CTCAGCAGGC ACTGCATATA 
CTGTCCACAT TGCAGGAGCC TCATGACAAG CACCTTCTGG ACAAAATTAA TGAATATATG 
GGCAAAGCTG CTACTCGTTT ACCCACTCTC TCGCTGCTGG GACATGTGAT AAGGCGACAA 
CCATCGTGGA AACATAAGCT TTCTCAAGCA CCTCTCCTAC TTTCATTACT TAAATGTCTC 
AAGACTGACA CAGATGTAGT AGTACTCACC ACAGGTGTCC TAGTGCTAAT AACCATGTTG 
CCAATGATTC CTCAGTCTGG CAAACAGTAC CTCCATGATT TCTTTGGTAT CTTTGGGCGT 
CTCTCAGCCT GGTGCTTGCA GAATCCAGGT CACGTGGCAG AGATTTACCT TGTCCATCTT 
CATGCCAGTG TTTATGCTCT CTTTCATCGG CTTTATGGAA TGTATCCTTG CAATTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACTTGG AGACCTTTGA AGAGGTAGTC 
AAGCCAATGA TGGAACATGT GCGAATTCAT CCAGAATTAG TGACTGGATC TAAGGACCAT 
GAACTGGACC CACGAAGGTG GAAAAGACTA GAAACTCATG ATGTTGTGAT AGAATGTGCC 
AAAATCTCCC TGGACCCTGC AGAAGCCTCA TATGAAGATG GCTATTACTC TGCGTCTCGG 
AAATCCTGTA CAAACTTAAA ACATCATCAA ACTGACCCCA GTGCCAGCCA TTACATTGAC 
ACACAGAGCA GCTATGGGAC GTCTACCCCA TATTCCACTC CTCGGCTAAC ACTATCACAA 
CTGCCAGGGC AGCTACCTCA GATTCTGAGC CCGCAGTCAA TAAAGCTGTC AACTGAGCCA 
CAGCAGGTTA CCATCTGGAG TCCTTCTGCA GTTTGTGGTA TGACCACTCC ACCAACCTCC 
CCTGGAATTG TCCCATCAGA GTCATCCCAG TCAACATCAC AACCTTACAG CAAAGCTTTT 
GGCACATCTG CAGTGGGGAA GGGAACACCT TTGGGGACAC CAGCCACATC TCCTCCACCC 
TGCACTTCAG ATGACTTTGT GCATGTCTCA CTTCCTTCAG CTGCTGCCAC ACCTCCTAAA 
AAGGAGGACA AACCAGATTC TGGGAGGCCT TCACTGTACC GACAGCAAAA TGTCATAAGC 
AGCGATAAAT CATTGGACAC ATCTGGTAGT AAAAGTTCAG TAACCTTAAG TGATCTTCCG 
GAGTTTTTAG GCGGTTTGTC TTTTGAAGAT AGTGCTGAAA AGGACAGAGA AGAAGATGCA 
ATATCTAAAG AGATCTCTGA GATCACGACT GATGCTGAAC ACATGGTGCC TAGAGGAGGA 
TTTGACTCTC CATTTTATCA CACAAATGAA AATCTGTCAG GTTCTCAGAA GAAGACCCAG 
TCAGTAGTCT CTAGTGTGCA GGGACACAGT CTGACCTCTG AGCCTTTAAC GTCTTCTCTG 
GACAAGCCTG GGCCTGAGAG TGCACTGGAG ACACCCAAAC AAACATTTAC TCCCATAGAC 
AAGCCCTGCG GAGGCTCTGG TGAAAGCCCT GCTGGTAACA GGGAAGGAAC CTCTGGGGAG 
ACAAGTATTC TCACTCCCAG CCCTTGCAAA GCATCAGCAC AAGGAAGAGC AGTGTTTGGG 
AGTGGGCAGC CTCCCCTGTA TGAGCACCTT TTTGAGGTTG CGCTACCAAA GACTGCCTAC 
CTCTTTGTTG GCAAGAAGAC TGAGGAGCTG CTAAAGAAAG CCAAGGGAAC CCAGGATAAA 
GACTGCATGT CCTCTACTTC TCCTGTGGAA GTACTGGACA GACTGATACA GCAAGGGGCA 
GATGCACACA CTAAGGAACT GAACAAATTG TCTCTGCCAA GCAAATCTGC TGACTGGACT 
CACTTTGGAG GTTCTCCCCC TTCAGATGAG ATTCACACCC TGCGTAACCA ATTGCTGTTG 
CTGCACAACC AGTTGCTGTA TGAACGCTTC AAAAGACAGC AGCATGCCCT TCGGAACCGG 
CGGCTCTTGC GGAAAGTGAT TAAAGCCACA GCACTGGAGG AACATAATGC TGCCATGAAA 
GATCAGCTGA AATTACAGGA AAAAGAAATC CAGGCCTTGA AACTGAGTCT GCAGAAAGAA 
CAGGCCAGGT ACCACCAGTT TCAGGAGGAG CACGAAGGGA TAGTGGCTCA GCTTCACAGC 
CAGATCAGAC AGCTGCAACA TGACCGTGAG GAATTCTACA ACCAGAGCCA GGAATTGCAG 
ACCAAGCTGG AAGACTGCAG GAACATGATT GCAGATCTGA GGTTAGAATT GAAAAAGGCT 
AACAACAAAG TCTGTCACAC AGAACTGCTT CTTAGCCAAG TTTCTCAAAA GCTTTCCAAC 
AGTGAATCAG TGCAACAGCA GATGGAGTTC TTGAACAGAC AACTTCTGGT TCTTGGAGAG 
GTCAATGAGT TGTACCTGGA GCAGCTGCAG CACAAGCACA CAGACACTAC AAAGGTTGAA 
ATGTTGCATG CTGCTTTTCG AAAGGAACTG GAGAAAGCAA AATTGTGTGT TCAGCAGCAA 
AGCCAAAGGC TCGATGCTTC TCAGAAACGG ATAGCTGAAC TGGAATCTCA GCTTTCTAAA 
AAGGACCACC TCTTCTTGGA GCAAAAGAAA TACCTGGAAG ATGTCAAAAT CAAAGCAAGA 
GGTCAGTTGC AAGCAGTAGA GAGTAGATAC AGGGCCCAGA AAAGGATCAC CCAGGCGTTT 
GAGCTGGAGA TCTTGAACCT GTTTGGCCGA CTGGAGAACA GCCCGCTGAA AAAACTTGAA 
GATGAAAAAA CAGAAGCAGC TGAAGCAGCA GAAGAAAGGC TGGATTGTGG TAATGAAGGT 
TGTGCAGATA CTGTGGCAGG ATACAGCGAA GAAACCATTG ATAGAAATGG AGAAACCAAA 
CCTCCCAGCA CTCGAGGTAG CAGTAAAGGT GGCAGCAACA GTGAGCTCTC CACCCCAGAA 
AAAAAACAGA ACCAGAGATT CAGCAGTCAC TGGGAGACCT CCATGTTGCG GGAGCCCTCC 
ACTACTGTCC CACTGACTGT AGGTTCACTC CCCAGCTCCA AGAGCTTCCT TGGTATGAAG 
TCACGAGAGT TGTTTCGCAA CAAAAGTGAG AGCCAGTGTG ATGAGCATGG TGTAACCATC 
AACAGCCTTT CTGATACTCT AAAGACTGAA CTATGTAAAG ATCCAAGCAT GGAGACGAAG 
GCTCCTCCAA GCCCCGATAA CCTTAGCCTC TCACCTAAGA TCCAGGAAAG CAGTGTTGGA 
CAGCTTCATA TCATGGACTA CAATGAAACT CATCATGACC ACAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_004945923.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004945980.1 Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OGa43674
Clone ID Related Accession (Same CDS sequence)	XM_004945923.3 , XM_004945923.2
Accession Version	XM_004945923.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	hamartin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004945923.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGC AGGGGAATGT TGGTGAGCTC CTCTCAATGC TGGATTCTCC AATTCTGGGT 
GTCCTGGAAG ACATAACAGC TGCATTCAAA GATAATCTCA TTTGTGACCG TGGACCTATG 
CTTGTGAACA GCCTGGTAGA CTATTACTTG GAAACCAATT CTCAGCAGGC ACTGCATATA 
CTGTCCACAT TGCAGGAGCC TCATGACAAG CACCTTCTGG ACAAAATTAA TGAATATATG 
GGCAAAGCTG CTACTCGTTT ACCCACTCTC TCGCTGCTGG GACATGTGAT AAGGCGACAA 
CCATCGTGGA AACATAAGCT TTCTCAAGCA CCTCTCCTAC TTTCATTACT TAAATGTCTC 
AAGACTGACA CAGATGTAGT AGTACTCACC ACAGGTGTCC TAGTGCTAAT AACCATGTTG 
CCAATGATTC CTCAGTCTGG CAAACAGTAC CTCCATGATT TCTTTGGTAT CTTTGGGCGT 
CTCTCAGCCT GGTGCTTGCA GAATCCAGGT CACGTGGCAG AGATTTACCT TGTCCATCTT 
CATGCCAGTG TTTATGCTCT CTTTCATCGG CTTTATGGAA TGTATCCTTG CAATTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACTTGG AGACCTTTGA AGAGGTAGTC 
AAGCCAATGA TGGAACATGT GCGAATTCAT CCAGAATTAG TGACTGGATC TAAGGACCAT 
GAACTGGACC CACGAAGGTG GAAAAGACTA GAAACTCATG ATGTTGTGAT AGAATGTGCC 
AAAATCTCCC TGGACCCTGC AGAAGCCTCA TATGAAGATG GCTATTACTC TGCGTCTCGG 
AAATCCTGTA CAAACTTAAA ACATCATCAA ACTGACCCCA GTGCCAGCCA TTACATTGAC 
ACACAGAGCA GCTATGGGAC GTCTACCCCA TATTCCACTC CTCGGCTAAC ACTATCACAA 
CTGCCAGGGC AGCTACCTCA GATTCTGAGC CCGCAGTCAA TAAAGCTGTC AACTGAGCCA 
CAGCAGGTTA CCATCTGGAG TCCTTCTGCA GTTTGTGGTA TGACCACTCC ACCAACCTCC 
CCTGGAATTG TCCCATCAGA GTCATCCCAG TCAACATCAC AACCTTACAG CAAAGCTTTT 
GGCACATCTG CAGTGGGGAA GGGAACACCT TTGGGGACAC CAGCCACATC TCCTCCACCC 
TGCACTTCAG ATGACTTTGT GCATGTCTCA CTTCCTTCAG CTGCTGCCAC ACCTCCTAAA 
AAGGAGGACA AACCAGATTC TGGGAGGCCT TCACTGTACC GACAGCAAAA TGTCATAAGC 
AGCGATAAAT CATTGGACAC ATCTGGTAGT AAAAGTTCAG TAACCTTAAG TGATCTTCCG 
GAGTTTTTAG GCGGTTTGTC TTTTGAAGAT AGTGCTGAAA AGGACAGAGA AGAAGATGCA 
ATATCTAAAG AGATCTCTGA GATCACGACT GATGCTGAAC ACATGGTGCC TAGAGGAGGA 
TTTGACTCTC CATTTTATCA CACAAATGAA AATCTGTCAG GTTCTCAGAA GAAGACCCAG 
TCAGTAGTCT CTAGTGTGCA GGGACACAGT CTGACCTCTG AGCCTTTAAC GTCTTCTCTG 
GACAAGCCTG GGCCTGAGAG TGCACTGGAG ACACCCAAAC AAACATTTAC TCCCATAGAC 
AAGCCCTGCG GAGGCTCTGG TGAAAGCCCT GCTGGTAACA GGGAAGGAAC CTCTGGGGAG 
ACAAGTATTC TCACTCCCAG CCCTTGCAAA GCATCAGCAC AAGGAAGAGC AGTGTTTGGG 
AGTGGGCAGC CTCCCCTGTA TGAGCACCTT TTTGAGGTTG CGCTACCAAA GACTGCCTAC 
CTCTTTGTTG GCAAGAAGAC TGAGGAGCTG CTAAAGAAAG CCAAGGGAAC CCAGGATAAA 
GACTGCATGT CCTCTACTTC TCCTGTGGAA GTACTGGACA GACTGATACA GCAAGGGGCA 
GATGCACACA CTAAGGAACT GAACAAATTG TCTCTGCCAA GCAAATCTGC TGACTGGACT 
CACTTTGGAG ATGAGATTCA CACCCTGCGT AACCAATTGC TGTTGCTGCA CAACCAGTTG 
CTGTATGAAC GCTTCAAAAG ACAGCAGCAT GCCCTTCGGA ACCGGCGGCT CTTGCGGAAA 
GTGATTAAAG CCACAGCACT GGAGGAACAT AATGCTGCCA TGAAAGATCA GCTGAAATTA 
CAGGAAAAAG AAATCCAGGC CTTGAAACTG AGTCTGCAGA AAGAACAGGC CAGGTACCAC 
CAGTTTCAGG AGGAGCACGA AGGGATAGTG GCTCAGCTTC ACAGCCAGAT CAGACAGCTG 
CAACATGACC GTGAGGAATT CTACAACCAG AGCCAGGAAT TGCAGACCAA GCTGGAAGAC 
TGCAGGAACA TGATTGCAGA TCTGAGGTTA GAATTGAAAA AGGCTAACAA CAAAGTCTGT 
CACACAGAAC TGCTTCTTAG CCAAGTTTCT CAAAAGCTTT CCAACAGTGA ATCAGTGCAA 
CAGCAGATGG AGTTCTTGAA CAGACAACTT CTGGTTCTTG GAGAGGTCAA TGAGTTGTAC 
CTGGAGCAGC TGCAGCACAA GCACACAGAC ACTACAAAGG AGGTTGAAAT GTTGCATGCT 
GCTTTTCGAA AGGAACTGGA GAAAGCAAAA TTGTGTGTTC AGCAGCAAAG CCAAAGGCTC 
GATGCTTCTC AGAAACGGAT AGCTGAACTG GAATCTCAGC TTTCTAAAAA GGACCACCTC 
TTCTTGGAGC AAAAGAAATA CCTGGAAGAT GTCAAAATCA AAGCAAGAGG TCAGTTGCAA 
GCAGTAGAGA GTAGATACAG GGCCCAGAAA AGGATCACCC AGGCGTTTGA GCTGGAGATC 
TTGAACCTGT TTGGCCGACT GGAGAACAGC CCGCTGAAAA AACTTGAAGA TGAAAAAACA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTG GATTGTGGTA ATGAAGGTTG TGCAGATACT 
GTGGCAGGAT ACAGCGAAGA AACCATTGAT AGAAATGGAG AAACCAAACC TCCCAGCACT 
CGAGGTAGCA GTAAAGGTGG CAGCAACAGT GAGCTCTCCA CCCCAGAAAA AAAACAGAAC 
CAGAGATTCA GCAGTCACTG GGAGACCTCC ATGTTGCGGG AGCCCTCCAC TACTGTCCCA 
CTGACTGTAG GTTCACTCCC CAGCTCCAAG AGCTTCCTTG GTATGAAGTC ACGAGAGTTG 
TTTCGCAACA AAAGTGAGAG CCAGTGTGAT GAGCATGGTG TAACCATCAA CAGCCTTTCT 
GATACTCTAA AGACTGAACT ATGTAAAGAT CCAAGCATGG AGACGAAGGC TCCTCCAAGC 
CCCGATAACC TTAGCCTCTC ACCTAAGATC CAGGAAAGCA GTGTTGGACA GCTTCATATC 
ATGGACTACA ATGAAACTCA TCATGACCAC AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004945980.1
CDS	224..3679
Translation	MAQQGNVGELLSMLDSPILGVLEDITAAFKDNLICDRGPMLVNSLVDYYLETNSQQALHILSTLQEPHDKHLLDKINEYMGKAATRLPTLSLLGHVIRRQPSWKHKLSQAPLLLSLLKCLKTDTDVVVLTTGVLVLITMLPMIPQSGKQYLHDFFGIFGRLSAWCLQNPGHVAEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPAEASYEDGYYSASRKSCTNLKHHQTDPSASHYIDTQSSYGTSTPYSTPRLTLSQLPGQLPQILSPQSIKLSTEPQQVTIWSPSAVCGMTTPPTSPGIVPSESSQSTSQPYSKAFGTSAVGKGTPLGTPATSPPPCTSDDFVHVSLPSAAATPPKKEDKPDSGRPSLYRQQNVISSDKSLDTSGSKSSVTLSDLPEFLGGLSFEDSAEKDREEDAISKEISEITTDAEHMVPRGGFDSPFYHTNENLSGSQKKTQSVVSSVQGHSLTSEPLTSSLDKPGPESALETPKQTFTPIDKPCGGSGESPAGNREGTSGETSILTPSPCKASAQGRAVFGSGQPPLYEHLFEVALPKTAYLFVGKKTEELLKKAKGTQDKDCMSSTSPVEVLDRLIQQGADAHTKELNKLSLPSKSADWTHFGDEIHTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKATALEEHNAAMKDQLKLQEKEIQALKLSLQKEQARYHQFQEEHEGIVAQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIADLRLELKKANNKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQHKHTDTTKEVEMLHAAFRKELEKAKLCVQQQSQRLDASQKRIAELESQLSKKDHLFLEQKKYLEDVKIKARGQLQAVESRYRAQKRITQAFELEILNLFGRLENSPLKKLEDEKTEAAEAAEERLDCGNEGCADTVAGYSEETIDRNGETKPPSTRGSSKGGSNSELSTPEKKQNQRFSSHWETSMLREPSTTVPLTVGSLPSSKSFLGMKSRELFRNKSESQCDEHGVTINSLSDTLKTELCKDPSMETKAPPSPDNLSLSPKIQESSVGQLHIMDYNETHHDHS

Target ORF information:

RefSeq Version	XM_004945923.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004945923.3

ORF Insert Sequence:

ATGGCACAGC AGGGGAATGT TGGTGAGCTC CTCTCAATGC TGGATTCTCC AATTCTGGGT 
GTCCTGGAAG ACATAACAGC TGCATTCAAA GATAATCTCA TTTGTGACCG TGGACCTATG 
CTTGTGAACA GCCTGGTAGA CTATTACTTG GAAACCAATT CTCAGCAGGC ACTGCATATA 
CTGTCCACAT TGCAGGAGCC TCATGACAAG CACCTTCTGG ACAAAATTAA TGAATATATG 
GGCAAAGCTG CTACTCGTTT ACCCACTCTC TCGCTGCTGG GACATGTGAT AAGGCGACAA 
CCATCGTGGA AACATAAGCT TTCTCAAGCA CCTCTCCTAC TTTCATTACT TAAATGTCTC 
AAGACTGACA CAGATGTAGT AGTACTCACC ACAGGTGTCC TAGTGCTAAT AACCATGTTG 
CCAATGATTC CTCAGTCTGG CAAACAGTAC CTCCATGATT TCTTTGGTAT CTTTGGGCGT 
CTCTCAGCCT GGTGCTTGCA GAATCCAGGT CACGTGGCAG AGATTTACCT TGTCCATCTT 
CATGCCAGTG TTTATGCTCT CTTTCATCGG CTTTATGGAA TGTATCCTTG CAATTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACTTGG AGACCTTTGA AGAGGTAGTC 
AAGCCAATGA TGGAACATGT GCGAATTCAT CCAGAATTAG TGACTGGATC TAAGGACCAT 
GAACTGGACC CACGAAGGTG GAAAAGACTA GAAACTCATG ATGTTGTGAT AGAATGTGCC 
AAAATCTCCC TGGACCCTGC AGAAGCCTCA TATGAAGATG GCTATTACTC TGCGTCTCGG 
AAATCCTGTA CAAACTTAAA ACATCATCAA ACTGACCCCA GTGCCAGCCA TTACATTGAC 
ACACAGAGCA GCTATGGGAC GTCTACCCCA TATTCCACTC CTCGGCTAAC ACTATCACAA 
CTGCCAGGGC AGCTACCTCA GATTCTGAGC CCGCAGTCAA TAAAGCTGTC AACTGAGCCA 
CAGCAGGTTA CCATCTGGAG TCCTTCTGCA GTTTGTGGTA TGACCACTCC ACCAACCTCC 
CCTGGAATTG TCCCATCAGA GTCATCCCAG TCAACATCAC AACCTTACAG CAAAGCTTTT 
GGCACATCTG CAGTGGGGAA GGGAACACCT TTGGGGACAC CAGCCACATC TCCTCCACCC 
TGCACTTCAG ATGACTTTGT GCATGTCTCA CTTCCTTCAG CTGCTGCCAC ACCTCCTAAA 
AAGGAGGACA AACCAGATTC TGGGAGGCCT TCACTGTACC GACAGCAAAA TGTCATAAGC 
AGCGATAAAT CATTGGACAC ATCTGGTAGT AAAAGTTCAG TAACCTTAAG TGATCTTCCG 
GAGTTTTTAG GCGGTTTGTC TTTTGAAGAT AGTGCTGAAA AGGACAGAGA AGAAGATGCA 
ATATCTAAAG AGATCTCTGA GATCACGACT GATGCTGAAC ACATGGTGCC TAGAGGAGGA 
TTTGACTCTC CATTTTATCA CACAAATGAA AATCTGTCAG GTTCTCAGAA GAAGACCCAG 
TCAGTAGTCT CTAGTGTGCA GGGACACAGT CTGACCTCTG AGCCTTTAAC GTCTTCTCTG 
GACAAGCCTG GGCCTGAGAG TGCACTGGAG ACACCCAAAC AAACATTTAC TCCCATAGAC 
AAGCCCTGCG GAGGCTCTGG TGAAAGCCCT GCTGGTAACA GGGAAGGAAC CTCTGGGGAG 
ACAAGTATTC TCACTCCCAG CCCTTGCAAA GCATCAGCAC AAGGAAGAGC AGTGTTTGGG 
AGTGGGCAGC CTCCCCTGTA TGAGCACCTT TTTGAGGTTG CGCTACCAAA GACTGCCTAC 
CTCTTTGTTG GCAAGAAGAC TGAGGAGCTG CTAAAGAAAG CCAAGGGAAC CCAGGATAAA 
GACTGCATGT CCTCTACTTC TCCTGTGGAA GTACTGGACA GACTGATACA GCAAGGGGCA 
GATGCACACA CTAAGGAACT GAACAAATTG TCTCTGCCAA GCAAATCTGC TGACTGGACT 
CACTTTGGAG ATGAGATTCA CACCCTGCGT AACCAATTGC TGTTGCTGCA CAACCAGTTG 
CTGTATGAAC GCTTCAAAAG ACAGCAGCAT GCCCTTCGGA ACCGGCGGCT CTTGCGGAAA 
GTGATTAAAG CCACAGCACT GGAGGAACAT AATGCTGCCA TGAAAGATCA GCTGAAATTA 
CAGGAAAAAG AAATCCAGGC CTTGAAACTG AGTCTGCAGA AAGAACAGGC CAGGTACCAC 
CAGTTTCAGG AGGAGCACGA AGGGATAGTG GCTCAGCTTC ACAGCCAGAT CAGACAGCTG 
CAACATGACC GTGAGGAATT CTACAACCAG AGCCAGGAAT TGCAGACCAA GCTGGAAGAC 
TGCAGGAACA TGATTGCAGA TCTGAGGTTA GAATTGAAAA AGGCTAACAA CAAAGTCTGT 
CACACAGAAC TGCTTCTTAG CCAAGTTTCT CAAAAGCTTT CCAACAGTGA ATCAGTGCAA 
CAGCAGATGG AGTTCTTGAA CAGACAACTT CTGGTTCTTG GAGAGGTCAA TGAGTTGTAC 
CTGGAGCAGC TGCAGCACAA GCACACAGAC ACTACAAAGG AGGTTGAAAT GTTGCATGCT 
GCTTTTCGAA AGGAACTGGA GAAAGCAAAA TTGTGTGTTC AGCAGCAAAG CCAAAGGCTC 
GATGCTTCTC AGAAACGGAT AGCTGAACTG GAATCTCAGC TTTCTAAAAA GGACCACCTC 
TTCTTGGAGC AAAAGAAATA CCTGGAAGAT GTCAAAATCA AAGCAAGAGG TCAGTTGCAA 
GCAGTAGAGA GTAGATACAG GGCCCAGAAA AGGATCACCC AGGCGTTTGA GCTGGAGATC 
TTGAACCTGT TTGGCCGACT GGAGAACAGC CCGCTGAAAA AACTTGAAGA TGAAAAAACA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTG GATTGTGGTA ATGAAGGTTG TGCAGATACT 
GTGGCAGGAT ACAGCGAAGA AACCATTGAT AGAAATGGAG AAACCAAACC TCCCAGCACT 
CGAGGTAGCA GTAAAGGTGG CAGCAACAGT GAGCTCTCCA CCCCAGAAAA AAAACAGAAC 
CAGAGATTCA GCAGTCACTG GGAGACCTCC ATGTTGCGGG AGCCCTCCAC TACTGTCCCA 
CTGACTGTAG GTTCACTCCC CAGCTCCAAG AGCTTCCTTG GTATGAAGTC ACGAGAGTTG 
TTTCGCAACA AAAGTGAGAG CCAGTGTGAT GAGCATGGTG TAACCATCAA CAGCCTTTCT 
GATACTCTAA AGACTGAACT ATGTAAAGAT CCAAGCATGG AGACGAAGGC TCCTCCAAGC 
CCCGATAACC TTAGCCTCTC ACCTAAGATC CAGGAAAGCA GTGTTGGACA GCTTCATATC 
ATGGACTACA ATGAAACTCA TCATGACCAC AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_004945923.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004945980.1 Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X4, mRNA.

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CloneID	OGa43674
Clone ID Related Accession (Same CDS sequence)	XM_004945923.3 , XM_004945923.2
Accession Version	XM_004945923.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	hamartin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004945923.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGC AGGGGAATGT TGGTGAGCTC CTCTCAATGC TGGATTCTCC AATTCTGGGT 
GTCCTGGAAG ACATAACAGC TGCATTCAAA GATAATCTCA TTTGTGACCG TGGACCTATG 
CTTGTGAACA GCCTGGTAGA CTATTACTTG GAAACCAATT CTCAGCAGGC ACTGCATATA 
CTGTCCACAT TGCAGGAGCC TCATGACAAG CACCTTCTGG ACAAAATTAA TGAATATATG 
GGCAAAGCTG CTACTCGTTT ACCCACTCTC TCGCTGCTGG GACATGTGAT AAGGCGACAA 
CCATCGTGGA AACATAAGCT TTCTCAAGCA CCTCTCCTAC TTTCATTACT TAAATGTCTC 
AAGACTGACA CAGATGTAGT AGTACTCACC ACAGGTGTCC TAGTGCTAAT AACCATGTTG 
CCAATGATTC CTCAGTCTGG CAAACAGTAC CTCCATGATT TCTTTGGTAT CTTTGGGCGT 
CTCTCAGCCT GGTGCTTGCA GAATCCAGGT CACGTGGCAG AGATTTACCT TGTCCATCTT 
CATGCCAGTG TTTATGCTCT CTTTCATCGG CTTTATGGAA TGTATCCTTG CAATTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACTTGG AGACCTTTGA AGAGGTAGTC 
AAGCCAATGA TGGAACATGT GCGAATTCAT CCAGAATTAG TGACTGGATC TAAGGACCAT 
GAACTGGACC CACGAAGGTG GAAAAGACTA GAAACTCATG ATGTTGTGAT AGAATGTGCC 
AAAATCTCCC TGGACCCTGC AGAAGCCTCA TATGAAGATG GCTATTACTC TGCGTCTCGG 
AAATCCTGTA CAAACTTAAA ACATCATCAA ACTGACCCCA GTGCCAGCCA TTACATTGAC 
ACACAGAGCA GCTATGGGAC GTCTACCCCA TATTCCACTC CTCGGCTAAC ACTATCACAA 
CTGCCAGGGC AGCTACCTCA GATTCTGAGC CCGCAGTCAA TAAAGCTGTC AACTGAGCCA 
CAGCAGGTTA CCATCTGGAG TCCTTCTGCA GTTTGTGGTA TGACCACTCC ACCAACCTCC 
CCTGGAATTG TCCCATCAGA GTCATCCCAG TCAACATCAC AACCTTACAG CAAAGCTTTT 
GGCACATCTG CAGTGGGGAA GGGAACACCT TTGGGGACAC CAGCCACATC TCCTCCACCC 
TGCACTTCAG ATGACTTTGT GCATGTCTCA CTTCCTTCAG CTGCTGCCAC ACCTCCTAAA 
AAGGAGGACA AACCAGATTC TGGGAGGCCT TCACTGTACC GACAGCAAAA TGTCATAAGC 
AGCGATAAAT CATTGGACAC ATCTGGTAGT AAAAGTTCAG TAACCTTAAG TGATCTTCCG 
GAGTTTTTAG GCGGTTTGTC TTTTGAAGAT AGTGCTGAAA AGGACAGAGA AGAAGATGCA 
ATATCTAAAG AGATCTCTGA GATCACGACT GATGCTGAAC ACATGGTGCC TAGAGGAGGA 
TTTGACTCTC CATTTTATCA CACAAATGAA AATCTGTCAG GTTCTCAGAA GAAGACCCAG 
TCAGTAGTCT CTAGTGTGCA GGGACACAGT CTGACCTCTG AGCCTTTAAC GTCTTCTCTG 
GACAAGCCTG GGCCTGAGAG TGCACTGGAG ACACCCAAAC AAACATTTAC TCCCATAGAC 
AAGCCCTGCG GAGGCTCTGG TGAAAGCCCT GCTGGTAACA GGGAAGGAAC CTCTGGGGAG 
ACAAGTATTC TCACTCCCAG CCCTTGCAAA GCATCAGCAC AAGGAAGAGC AGTGTTTGGG 
AGTGGGCAGC CTCCCCTGTA TGAGCACCTT TTTGAGGTTG CGCTACCAAA GACTGCCTAC 
CTCTTTGTTG GCAAGAAGAC TGAGGAGCTG CTAAAGAAAG CCAAGGGAAC CCAGGATAAA 
GACTGCATGT CCTCTACTTC TCCTGTGGAA GTACTGGACA GACTGATACA GCAAGGGGCA 
GATGCACACA CTAAGGAACT GAACAAATTG TCTCTGCCAA GCAAATCTGC TGACTGGACT 
CACTTTGGAG ATGAGATTCA CACCCTGCGT AACCAATTGC TGTTGCTGCA CAACCAGTTG 
CTGTATGAAC GCTTCAAAAG ACAGCAGCAT GCCCTTCGGA ACCGGCGGCT CTTGCGGAAA 
GTGATTAAAG CCACAGCACT GGAGGAACAT AATGCTGCCA TGAAAGATCA GCTGAAATTA 
CAGGAAAAAG AAATCCAGGC CTTGAAACTG AGTCTGCAGA AAGAACAGGC CAGGTACCAC 
CAGTTTCAGG AGGAGCACGA AGGGATAGTG GCTCAGCTTC ACAGCCAGAT CAGACAGCTG 
CAACATGACC GTGAGGAATT CTACAACCAG AGCCAGGAAT TGCAGACCAA GCTGGAAGAC 
TGCAGGAACA TGATTGCAGA TCTGAGGTTA GAATTGAAAA AGGCTAACAA CAAAGTCTGT 
CACACAGAAC TGCTTCTTAG CCAAGTTTCT CAAAAGCTTT CCAACAGTGA ATCAGTGCAA 
CAGCAGATGG AGTTCTTGAA CAGACAACTT CTGGTTCTTG GAGAGGTCAA TGAGTTGTAC 
CTGGAGCAGC TGCAGCACAA GCACACAGAC ACTACAAAGG AGGTTGAAAT GTTGCATGCT 
GCTTTTCGAA AGGAACTGGA GAAAGCAAAA TTGTGTGTTC AGCAGCAAAG CCAAAGGCTC 
GATGCTTCTC AGAAACGGAT AGCTGAACTG GAATCTCAGC TTTCTAAAAA GGACCACCTC 
TTCTTGGAGC AAAAGAAATA CCTGGAAGAT GTCAAAATCA AAGCAAGAGG TCAGTTGCAA 
GCAGTAGAGA GTAGATACAG GGCCCAGAAA AGGATCACCC AGGCGTTTGA GCTGGAGATC 
TTGAACCTGT TTGGCCGACT GGAGAACAGC CCGCTGAAAA AACTTGAAGA TGAAAAAACA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTG GATTGTGGTA ATGAAGGTTG TGCAGATACT 
GTGGCAGGAT ACAGCGAAGA AACCATTGAT AGAAATGGAG AAACCAAACC TCCCAGCACT 
CGAGGTAGCA GTAAAGGTGG CAGCAACAGT GAGCTCTCCA CCCCAGAAAA AAAACAGAAC 
CAGAGATTCA GCAGTCACTG GGAGACCTCC ATGTTGCGGG AGCCCTCCAC TACTGTCCCA 
CTGACTGTAG GTTCACTCCC CAGCTCCAAG AGCTTCCTTG GTATGAAGTC ACGAGAGTTG 
TTTCGCAACA AAAGTGAGAG CCAGTGTGAT GAGCATGGTG TAACCATCAA CAGCCTTTCT 
GATACTCTAA AGACTGAACT ATGTAAAGAT CCAAGCATGG AGACGAAGGC TCCTCCAAGC 
CCCGATAACC TTAGCCTCTC ACCTAAGATC CAGGAAAGCA GTGTTGGACA GCTTCATATC 
ATGGACTACA ATGAAACTCA TCATGACCAC AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004945980.1
CDS	219..3674
Translation	MAQQGNVGELLSMLDSPILGVLEDITAAFKDNLICDRGPMLVNSLVDYYLETNSQQALHILSTLQEPHDKHLLDKINEYMGKAATRLPTLSLLGHVIRRQPSWKHKLSQAPLLLSLLKCLKTDTDVVVLTTGVLVLITMLPMIPQSGKQYLHDFFGIFGRLSAWCLQNPGHVAEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPAEASYEDGYYSASRKSCTNLKHHQTDPSASHYIDTQSSYGTSTPYSTPRLTLSQLPGQLPQILSPQSIKLSTEPQQVTIWSPSAVCGMTTPPTSPGIVPSESSQSTSQPYSKAFGTSAVGKGTPLGTPATSPPPCTSDDFVHVSLPSAAATPPKKEDKPDSGRPSLYRQQNVISSDKSLDTSGSKSSVTLSDLPEFLGGLSFEDSAEKDREEDAISKEISEITTDAEHMVPRGGFDSPFYHTNENLSGSQKKTQSVVSSVQGHSLTSEPLTSSLDKPGPESALETPKQTFTPIDKPCGGSGESPAGNREGTSGETSILTPSPCKASAQGRAVFGSGQPPLYEHLFEVALPKTAYLFVGKKTEELLKKAKGTQDKDCMSSTSPVEVLDRLIQQGADAHTKELNKLSLPSKSADWTHFGDEIHTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKATALEEHNAAMKDQLKLQEKEIQALKLSLQKEQARYHQFQEEHEGIVAQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIADLRLELKKANNKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQHKHTDTTKEVEMLHAAFRKELEKAKLCVQQQSQRLDASQKRIAELESQLSKKDHLFLEQKKYLEDVKIKARGQLQAVESRYRAQKRITQAFELEILNLFGRLENSPLKKLEDEKTEAAEAAEERLDCGNEGCADTVAGYSEETIDRNGETKPPSTRGSSKGGSNSELSTPEKKQNQRFSSHWETSMLREPSTTVPLTVGSLPSSKSFLGMKSRELFRNKSESQCDEHGVTINSLSDTLKTELCKDPSMETKAPPSPDNLSLSPKIQESSVGQLHIMDYNETHHDHS

Target ORF information:

RefSeq Version	XM_004945923.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tuberous sclerosis 1 (TSC1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004945923.2

ORF Insert Sequence:

ATGGCACAGC AGGGGAATGT TGGTGAGCTC CTCTCAATGC TGGATTCTCC AATTCTGGGT 
GTCCTGGAAG ACATAACAGC TGCATTCAAA GATAATCTCA TTTGTGACCG TGGACCTATG 
CTTGTGAACA GCCTGGTAGA CTATTACTTG GAAACCAATT CTCAGCAGGC ACTGCATATA 
CTGTCCACAT TGCAGGAGCC TCATGACAAG CACCTTCTGG ACAAAATTAA TGAATATATG 
GGCAAAGCTG CTACTCGTTT ACCCACTCTC TCGCTGCTGG GACATGTGAT AAGGCGACAA 
CCATCGTGGA AACATAAGCT TTCTCAAGCA CCTCTCCTAC TTTCATTACT TAAATGTCTC 
AAGACTGACA CAGATGTAGT AGTACTCACC ACAGGTGTCC TAGTGCTAAT AACCATGTTG 
CCAATGATTC CTCAGTCTGG CAAACAGTAC CTCCATGATT TCTTTGGTAT CTTTGGGCGT 
CTCTCAGCCT GGTGCTTGCA GAATCCAGGT CACGTGGCAG AGATTTACCT TGTCCATCTT 
CATGCCAGTG TTTATGCTCT CTTTCATCGG CTTTATGGAA TGTATCCTTG CAATTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACTTGG AGACCTTTGA AGAGGTAGTC 
AAGCCAATGA TGGAACATGT GCGAATTCAT CCAGAATTAG TGACTGGATC TAAGGACCAT 
GAACTGGACC CACGAAGGTG GAAAAGACTA GAAACTCATG ATGTTGTGAT AGAATGTGCC 
AAAATCTCCC TGGACCCTGC AGAAGCCTCA TATGAAGATG GCTATTACTC TGCGTCTCGG 
AAATCCTGTA CAAACTTAAA ACATCATCAA ACTGACCCCA GTGCCAGCCA TTACATTGAC 
ACACAGAGCA GCTATGGGAC GTCTACCCCA TATTCCACTC CTCGGCTAAC ACTATCACAA 
CTGCCAGGGC AGCTACCTCA GATTCTGAGC CCGCAGTCAA TAAAGCTGTC AACTGAGCCA 
CAGCAGGTTA CCATCTGGAG TCCTTCTGCA GTTTGTGGTA TGACCACTCC ACCAACCTCC 
CCTGGAATTG TCCCATCAGA GTCATCCCAG TCAACATCAC AACCTTACAG CAAAGCTTTT 
GGCACATCTG CAGTGGGGAA GGGAACACCT TTGGGGACAC CAGCCACATC TCCTCCACCC 
TGCACTTCAG ATGACTTTGT GCATGTCTCA CTTCCTTCAG CTGCTGCCAC ACCTCCTAAA 
AAGGAGGACA AACCAGATTC TGGGAGGCCT TCACTGTACC GACAGCAAAA TGTCATAAGC 
AGCGATAAAT CATTGGACAC ATCTGGTAGT AAAAGTTCAG TAACCTTAAG TGATCTTCCG 
GAGTTTTTAG GCGGTTTGTC TTTTGAAGAT AGTGCTGAAA AGGACAGAGA AGAAGATGCA 
ATATCTAAAG AGATCTCTGA GATCACGACT GATGCTGAAC ACATGGTGCC TAGAGGAGGA 
TTTGACTCTC CATTTTATCA CACAAATGAA AATCTGTCAG GTTCTCAGAA GAAGACCCAG 
TCAGTAGTCT CTAGTGTGCA GGGACACAGT CTGACCTCTG AGCCTTTAAC GTCTTCTCTG 
GACAAGCCTG GGCCTGAGAG TGCACTGGAG ACACCCAAAC AAACATTTAC TCCCATAGAC 
AAGCCCTGCG GAGGCTCTGG TGAAAGCCCT GCTGGTAACA GGGAAGGAAC CTCTGGGGAG 
ACAAGTATTC TCACTCCCAG CCCTTGCAAA GCATCAGCAC AAGGAAGAGC AGTGTTTGGG 
AGTGGGCAGC CTCCCCTGTA TGAGCACCTT TTTGAGGTTG CGCTACCAAA GACTGCCTAC 
CTCTTTGTTG GCAAGAAGAC TGAGGAGCTG CTAAAGAAAG CCAAGGGAAC CCAGGATAAA 
GACTGCATGT CCTCTACTTC TCCTGTGGAA GTACTGGACA GACTGATACA GCAAGGGGCA 
GATGCACACA CTAAGGAACT GAACAAATTG TCTCTGCCAA GCAAATCTGC TGACTGGACT 
CACTTTGGAG ATGAGATTCA CACCCTGCGT AACCAATTGC TGTTGCTGCA CAACCAGTTG 
CTGTATGAAC GCTTCAAAAG ACAGCAGCAT GCCCTTCGGA ACCGGCGGCT CTTGCGGAAA 
GTGATTAAAG CCACAGCACT GGAGGAACAT AATGCTGCCA TGAAAGATCA GCTGAAATTA 
CAGGAAAAAG AAATCCAGGC CTTGAAACTG AGTCTGCAGA AAGAACAGGC CAGGTACCAC 
CAGTTTCAGG AGGAGCACGA AGGGATAGTG GCTCAGCTTC ACAGCCAGAT CAGACAGCTG 
CAACATGACC GTGAGGAATT CTACAACCAG AGCCAGGAAT TGCAGACCAA GCTGGAAGAC 
TGCAGGAACA TGATTGCAGA TCTGAGGTTA GAATTGAAAA AGGCTAACAA CAAAGTCTGT 
CACACAGAAC TGCTTCTTAG CCAAGTTTCT CAAAAGCTTT CCAACAGTGA ATCAGTGCAA 
CAGCAGATGG AGTTCTTGAA CAGACAACTT CTGGTTCTTG GAGAGGTCAA TGAGTTGTAC 
CTGGAGCAGC TGCAGCACAA GCACACAGAC ACTACAAAGG AGGTTGAAAT GTTGCATGCT 
GCTTTTCGAA AGGAACTGGA GAAAGCAAAA TTGTGTGTTC AGCAGCAAAG CCAAAGGCTC 
GATGCTTCTC AGAAACGGAT AGCTGAACTG GAATCTCAGC TTTCTAAAAA GGACCACCTC 
TTCTTGGAGC AAAAGAAATA CCTGGAAGAT GTCAAAATCA AAGCAAGAGG TCAGTTGCAA 
GCAGTAGAGA GTAGATACAG GGCCCAGAAA AGGATCACCC AGGCGTTTGA GCTGGAGATC 
TTGAACCTGT TTGGCCGACT GGAGAACAGC CCGCTGAAAA AACTTGAAGA TGAAAAAACA 
GAAGCAGCTG AAGCAGCAGA AGAAAGGCTG GATTGTGGTA ATGAAGGTTG TGCAGATACT 
GTGGCAGGAT ACAGCGAAGA AACCATTGAT AGAAATGGAG AAACCAAACC TCCCAGCACT 
CGAGGTAGCA GTAAAGGTGG CAGCAACAGT GAGCTCTCCA CCCCAGAAAA AAAACAGAAC 
CAGAGATTCA GCAGTCACTG GGAGACCTCC ATGTTGCGGG AGCCCTCCAC TACTGTCCCA 
CTGACTGTAG GTTCACTCCC CAGCTCCAAG AGCTTCCTTG GTATGAAGTC ACGAGAGTTG 
TTTCGCAACA AAAGTGAGAG CCAGTGTGAT GAGCATGGTG TAACCATCAA CAGCCTTTCT 
GATACTCTAA AGACTGAACT ATGTAAAGAT CCAAGCATGG AGACGAAGGC TCCTCCAAGC 
CCCGATAACC TTAGCCTCTC ACCTAAGATC CAGGAAAGCA GTGTTGGACA GCTTCATATC 
ATGGACTACA ATGAAACTCA TCATGACCAC AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TSC1
Entrez Gene ID	417166
Full Name	tuberous sclerosis 1
General protein information	Preferred Names tuberous sclerosis 1 Names hamartin
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Homo sapiens (human)	TSC1	NP_000359.1
Bos taurus (cattle)	TSC1	XP_005213508.1
	TSC1	XP_001103846.2
Mus musculus (house mouse)	Tsc1	NP_075025.2
Xenopus tropicalis (tropical clawed frog)	tsc1	XP_002944681.2
Pan troglodytes (chimpanzee)	TSC1	XP_001168992.1
Gallus gallus (chicken)	TSC1	XP_415449.2
Canis lupus familiaris (dog)	TSC1	XP_005625233.1
Rattus norvegicus (Norway rat)	Tsc1	NP_068626.1
Danio rerio (zebrafish)	tsc1a	NP_956346.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TSC1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

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