Col5a3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col5a3
Entrez Gene ID	53867
Full Name	collagen, type V, alpha 3
General protein information	Preferred Names collagen, type V, alpha 3 Names collagen alpha-3(V) chain Pro-alpha3(V) procollagen, type V, alpha 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_016919.3	NP_058615.1	collagen alpha-3(V) chain isoform 1 precursor
XM_017313471.1	XP_017168960.1	collagen alpha-3(V) chain isoform X1
NM_001317388.1	NP_001304317.1	collagen alpha-3(V) chain isoform 2 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL5A3	XP_003953413.1
Mus musculus (house mouse)	Col5a3	NP_058615.1
Bos taurus (cattle)	COL5A3	NP_001192553.1
Homo sapiens (human)	COL5A3	NP_056534.2
Rattus norvegicus (Norway rat)	Col5a3	NP_068528.1
	COL5A3	XP_002808215.1
Canis lupus familiaris (dog)	COL5A3	XP_005632967.1

Gene Ontology
Bibliography

Related articles in PubMed

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Naba A, Clauser KR, Mani DR, Carr SA, Hynes RO
Scientific reports740495(2017 Jan)

?3 Chains of type V collagen regulate breast tumour growth via glypican-1.
Huang G, Ge G, Izzi V, Greenspan DS
Nature communications814351(2017 Jan)

The matrisome: in silico definition and in vivo characterization by proteomics of normal and tumor extracellular matrices.
Naba A, Clauser KR, Hoersch S, Liu H, Carr SA, Hynes RO
Molecular & cellular proteomics : MCP11(4)M111.014647(2012 Apr)

Transient expression of mouse pro-?3(V) collagen gene (Col5a3) in wound healing.
Sumiyoshi H, Kitamura H, Matsuo N, Tatsukawa S, Ishikawa K, Okamoto O, Fujikura Y, Fujiwara S, Yoshioka H
Connective tissue research53(4)313-7(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Present study examined the expression of pro-alpha3(V) collagen gene in a mouse model of wound healing.
Title: Transient expression of mouse pro-?3(V) collagen gene (Col5a3) in wound healing.

data show the alpha3(V) collagen chain to constitute an important element of the microenvironment of certain highly specialized cell types in white adipose tissue, skeletal muscle and pancreatic islets and to have profound effects on function of such cells
Title: ?3(V) collagen is critical for glucose homeostasis in mice due to effects in pancreatic islets and peripheral tissues.

The Sp1 and CBF/NF-Y transcription factors cooperatively regulate the mouse pro-alpha3(V) collagen gene (Col5a3) in osteoblastic cells.
Title: The Sp1 and CBF/NF-Y transcription factors cooperatively regulate the mouse pro-alpha3(V) collagen gene (Col5a3) in osteoblastic cells.

Sp7/Osterix promotes the expression of the collagen gene, Col5a3, and thereby playing a role in bone formation.
Title: Sp7/Osterix up-regulates the mouse pro-alpha3(V) collagen gene (Col5a3) during the osteoblast differentiation.

N-terminal globular domain of the pro-alpha3(V) chain influence bone formation of osteoblasts through proteoglycan on the cell surface during development or regeneration
Title: Pro-alpha3(V) collagen chain is expressed in bone and its basic N-terminal peptide adheres to osteosarcoma cells.

The following Col5a3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col5a3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu82886	XM_017313471.1 Latest version!	Mus musculus collagen, type V, alpha 3 (Col5a3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu10729	NM_016919.3 Latest version!	Mus musculus collagen, type V, alpha 3 (Col5a3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1280.30 $1829.00
OMu42318	NM_001317388.1 Latest version!	Mus musculus collagen, type V, alpha 3 (Col5a3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col5a3 ( XM_017313471.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168960.1 Mus musculus collagen, type V, alpha 3 (Col5a3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu82886
Clone ID Related Accession (Same CDS sequence)	XM_017313471.1
Accession Version	XM_017313471.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-3(V) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGCT GCCGGAGACT GGATCAGCTT CAGGCCGGCC TCTGCCTGCT CCTGGCCTCC 
CTGCAGCTCG TGTCCTGGAC GCTGGCTGCA GAACCTGTGG ACGTACTGGA AGCCTGGGGT 
GTGCATAGAG ACCAGGCTGG GGTGGCTGAA GGGCCTGGCT TCTGCCCCCT GAGGATTCCA 
CAGGGTGACC GAGCATTCAG GGTGGGCAAG TCCAGCCTTC TCAGTGTCCC CACGTGGCAG 
CTCTTCCCAG ATGGGCATTT TCCTGAGAAC TTTTCTGTGC TGCTCACACT GAGGGCCCAG 
CCAGCCAATC AGTCTGTCCT TCTGTCTATT TATGATGAGA AGGGTGTCCG GCAGCTGGGG 
CTGGCACTGG GGCCAGCTCT GGGCCTCCTT GGTGACTCCT TCAGGCCCCT CCCCAAGCAA 
GTCAACATTA TGGATGGCAG GTGGCACCGT GTGGCAGTCA GCATCAGTGG TAACAAGGTG 
ACCCTGGTGG TTGACTGTGA ACCGCAGCCC CCAACATTTG GTCAGGGGCC TCGGTTTATA 
AGTACAGCTG GACTCACTGT GATGGGAACC CAGGACACCA GGGAAGAGTC TTTTGAGGGA 
GACATCCAGG AGCTGCTGTT AATTCCAGAC CCTCAGGCTG CCTTCCAGGC CTGTGAGAGC 
TACCTCCCTG GTTGTGAAAC CCTCGATTCC ACAACCACAG GGGCCCCCAA AGACGATGAA 
CCAGAAACCC CTGCCCCTCG TCGTCGAAAG GGCAAAGGGA AGAAAAAAGG GCGGGGTCGA 
AAGGGCAAGG GAAGAAAGAA AAACAAGGAG ACCTCAGAGC TGAGTCCGAC CCCTGGTGCC 
CCTGAGAACC AGACCTCCCT CCACATCCCT GAGACAGAGA AGACAGTTCC CCACCTGCCT 
CTGACTCCCA CACCTCTGGC CATCACCACC ACTGTCACGA TTGGACAAAA TGCCACAGTC 
TCGCAGGGGT TGGACTCCGG TACTGAAACC GAGCAGACGA CTCCAGAGGT GGACTCTACT 
GAGGAGGGTG AAGGAGGTGG CCCCACCATG GGCCCCAAGT TCCGGGCAGC AGAGCAGTCC 
TTACAGACTG AGTTCCAGAT CTTTCCTGGT GCTGGAGAAA AGGGAGCGAA AGGAGAGCCT 
GCGACAGTAG AGCAGGGACA GCAGTTTGAG GGGCCTGCAG GAGCTCCAGG ACCCCGGGGA 
ATATCTGGTC CTTCAGGCCC TCCTGGGCCT CCGGGCTTCC CTGGGGACCG TGGTCTACCG 
GGTCCTGCCG GCCTCCCAGG AATCCCAGGC ATCGATGGAG CCCGGGGCCT GCCGGGCACA 
GTGATTATGA TGCCGTTCCA TTTTGCAAGC AGCTCGATGA AGGGACCCCC AGTGTCCTTC 
CAGCAGGCCC AGGCCCAGGC AGTATTGCAA CAGGCTCAGC TGTCCATGAA AGGGCCCCCT 
GGTCCAGTAG GGCTCACTGG GCGCCCAGGC CCTGTGGGCC TCCCTGGATA TCCAGGTCTG 
AAAGGTGAAC TGGGAGAAGT GGGGCCACAG GGCCCCCGAG GATTACAGGG CCCTCCTGGG 
CCTCCTGGAC GGGAAGGCAA GACAGGCCGA GCTGGAGCAG ATGGGGCTCG GGGGCTCCCG 
GGAGACACAG GACCTAAGGG TGACAGGGGC TTTGATGGCC TGCCCGGGCT GCCTGGTGAG 
AAGGGCCAAA GGGGTGACTT TGGACGAGTA GGGCAACCTG GTCCCCCAGG AGAGGATGGT 
GTAAAGGGCC TGCAGGGACC TCCAGGGCCC ACTGGCCAGG CTGGAGAGCC GGGTCCCCGA 
GGTCTGATTG GCCCCAGAGG TCTCCCAGGT CCCCTAGGAC GCCCGGGTGT GACAGGGAGT 
GATGGCGCAC CAGGGGCCAA AGGCAACGTG GGTCCTCCTG GAGAACCAGG ACCCCCAGGA 
CAGCAAGGAA ACCACGGCTC CCAGGGAATT CCAGGCCCCC AGGGGCCCAT TGGCACTCCC 
GGGGAAAAGG GTCCCCCTGG AAACCCCGGA ATTCCAGGTG TCCCAGGATC TGAGGGCCCC 
CCGGGCCACC CAGGCCACGA GGGTCCCACA GGAGAAAAAG GGGCTCAGGG CCCACCAGGA 
TCAGCAGGCC CTCGGGGCTA TCCTGGACTT CGTGGTGTGA AGGGTACCTC TGGTAACCGG 
GGTCTCCAAG GCGAGAAAGG AGAAAGGGGA GAGGATGGCT TTCCTGGCTT CAAGGGTGAT 
GAGGGACCAA AAGGCGACCG GGGAAACCCC GGACCCCCAG GTCCCAGAGG AGAGGATGGT 
CCAGAAGGAC AAAAGGGGCC TGGGGGACTG CCTGGTGATG AGGGTCCTCC AGGAGCAGCA 
GGGGAGAAGG GCAAGCTTGG GGTGCCAGGT CTCCCAGGTT ATCCAGGACG CCCAGGACCT 
AAGGGATCTA TTGGATTTCC TGGACCCTTG GGACCACTGG GGGAGAAAGG CAAGCGGGGC 
AAAGCAGGAC AGCCAGGAGA GGAAGGAGAA CGCGGCACAC CGGGCACCCG AGGAGACAGG 
GGACAGCCGG GGGCCACAGG CCAGCCTGGC CCCAAGGGTG ACGTGGGCCA GAATGGGTCT 
CCTGGGCCCC CTGGAGAAAA GGGTCTACCC GGTCTTCAAG GCCCACCAGG ATTCCCCGGA 
CCAAAAGGCC CCCCGGGTCC TCAGGGGAAA GATGGGATAT CTGGGCACCC TGGACAAAGA 
GGAGAATTGG GCTTCCAAGG TCTGACAGGC CCCCCTGGAC CAGCTGGCGT CCTTGGTCCT 
CAGGGAAAGG TAGGGGACGT GGGGCCTCTA GGCGAGAGAG GCCCCCCAGG GCCTCCTGGA 
CCTCCTGGTG AACAAGGTCT GCCAGGCATA GAAGGCAGAG AAGGGGCCAA GGGTGAGCTA 
GGACCCCTGG GGTCCGTCGG GAAGGAGGGG CCACCTGGGC CCAGGGGCTT CCCTGGCCCC 
CAAGGAGCCC CCGGAGACCC AGGACCCATT GGTTTGAAGG GTGACAAAGG TCCCCCAGGC 
CCTGTTGGGG CAAATGGCTC CCCGGGAGAG CGTGGTCCTG TAGGCCCCTC TGGTGGCATT 
GGGCTTCCTG GCCAGAGTGG AGGGCAAGGC CCTATTGGTC CTGCTGGCGA GAAGGGGTCC 
CCGGGAGAAC GGGGTACTCC TGGTCCTACT GGCAAAGATG GTATTCCAGG ACCCCCGGGG 
CTTCAGGGCC CCTCTGGAGC TGCGGGGCCT TCTGGGGAAG AAGGAGACAA GGGGGAAGTA 
GGGATGCCTG GTCACAAAGG AAGCAAAGGG GATAAAGGAG ATGCAGGCCC ACCTGGACCA 
ACAGGAATAA GAGGTCCAGC AGGCCATTCA GGCCTCCCGA AACAACTGTA TATACGATGC 
TGCATTTCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168960.1
CDS	165..3536
Translation	MRSCRRLDQLQAGLCLLLASLQLVSWTLAAEPVDVLEAWGVHRDQAGVAEGPGFCPLRIPQGDRAFRVGKSSLLSVPTWQLFPDGHFPENFSVLLTLRAQPANQSVLLSIYDEKGVRQLGLALGPALGLLGDSFRPLPKQVNIMDGRWHRVAVSISGNKVTLVVDCEPQPPTFGQGPRFISTAGLTVMGTQDTREESFEGDIQELLLIPDPQAAFQACESYLPGCETLDSTTTGAPKDDEPETPAPRRRKGKGKKKGRGRKGKGRKKNKETSELSPTPGAPENQTSLHIPETEKTVPHLPLTPTPLAITTTVTIGQNATVSQGLDSGTETEQTTPEVDSTEEGEGGGPTMGPKFRAAEQSLQTEFQIFPGAGEKGAKGEPATVEQGQQFEGPAGAPGPRGISGPSGPPGPPGFPGDRGLPGPAGLPGIPGIDGARGLPGTVIMMPFHFASSSMKGPPVSFQQAQAQAVLQQAQLSMKGPPGPVGLTGRPGPVGLPGYPGLKGELGEVGPQGPRGLQGPPGPPGREGKTGRAGADGARGLPGDTGPKGDRGFDGLPGLPGEKGQRGDFGRVGQPGPPGEDGVKGLQGPPGPTGQAGEPGPRGLIGPRGLPGPLGRPGVTGSDGAPGAKGNVGPPGEPGPPGQQGNHGSQGIPGPQGPIGTPGEKGPPGNPGIPGVPGSEGPPGHPGHEGPTGEKGAQGPPGSAGPRGYPGLRGVKGTSGNRGLQGEKGERGEDGFPGFKGDEGPKGDRGNPGPPGPRGEDGPEGQKGPGGLPGDEGPPGAAGEKGKLGVPGLPGYPGRPGPKGSIGFPGPLGPLGEKGKRGKAGQPGEEGERGTPGTRGDRGQPGATGQPGPKGDVGQNGSPGPPGEKGLPGLQGPPGFPGPKGPPGPQGKDGISGHPGQRGELGFQGLTGPPGPAGVLGPQGKVGDVGPLGERGPPGPPGPPGEQGLPGIEGREGAKGELGPLGSVGKEGPPGPRGFPGPQGAPGDPGPIGLKGDKGPPGPVGANGSPGERGPVGPSGGIGLPGQSGGQGPIGPAGEKGSPGERGTPGPTGKDGIPGPPGLQGPSGAAGPSGEEGDKGEVGMPGHKGSKGDKGDAGPPGPTGIRGPAGHSGLPKQLYIRCCIS

Target ORF information:

RefSeq Version	XM_017313471.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type V, alpha 3 (Col5a3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313471.1

ORF Insert Sequence:

ATGAGAAGCT GCCGGAGACT GGATCAGCTT CAGGCCGGCC TCTGCCTGCT CCTGGCCTCC 
CTGCAGCTCG TGTCCTGGAC GCTGGCTGCA GAACCTGTGG ACGTACTGGA AGCCTGGGGT 
GTGCATAGAG ACCAGGCTGG GGTGGCTGAA GGGCCTGGCT TCTGCCCCCT GAGGATTCCA 
CAGGGTGACC GAGCATTCAG GGTGGGCAAG TCCAGCCTTC TCAGTGTCCC CACGTGGCAG 
CTCTTCCCAG ATGGGCATTT TCCTGAGAAC TTTTCTGTGC TGCTCACACT GAGGGCCCAG 
CCAGCCAATC AGTCTGTCCT TCTGTCTATT TATGATGAGA AGGGTGTCCG GCAGCTGGGG 
CTGGCACTGG GGCCAGCTCT GGGCCTCCTT GGTGACTCCT TCAGGCCCCT CCCCAAGCAA 
GTCAACATTA TGGATGGCAG GTGGCACCGT GTGGCAGTCA GCATCAGTGG TAACAAGGTG 
ACCCTGGTGG TTGACTGTGA ACCGCAGCCC CCAACATTTG GTCAGGGGCC TCGGTTTATA 
AGTACAGCTG GACTCACTGT GATGGGAACC CAGGACACCA GGGAAGAGTC TTTTGAGGGA 
GACATCCAGG AGCTGCTGTT AATTCCAGAC CCTCAGGCTG CCTTCCAGGC CTGTGAGAGC 
TACCTCCCTG GTTGTGAAAC CCTCGATTCC ACAACCACAG GGGCCCCCAA AGACGATGAA 
CCAGAAACCC CTGCCCCTCG TCGTCGAAAG GGCAAAGGGA AGAAAAAAGG GCGGGGTCGA 
AAGGGCAAGG GAAGAAAGAA AAACAAGGAG ACCTCAGAGC TGAGTCCGAC CCCTGGTGCC 
CCTGAGAACC AGACCTCCCT CCACATCCCT GAGACAGAGA AGACAGTTCC CCACCTGCCT 
CTGACTCCCA CACCTCTGGC CATCACCACC ACTGTCACGA TTGGACAAAA TGCCACAGTC 
TCGCAGGGGT TGGACTCCGG TACTGAAACC GAGCAGACGA CTCCAGAGGT GGACTCTACT 
GAGGAGGGTG AAGGAGGTGG CCCCACCATG GGCCCCAAGT TCCGGGCAGC AGAGCAGTCC 
TTACAGACTG AGTTCCAGAT CTTTCCTGGT GCTGGAGAAA AGGGAGCGAA AGGAGAGCCT 
GCGACAGTAG AGCAGGGACA GCAGTTTGAG GGGCCTGCAG GAGCTCCAGG ACCCCGGGGA 
ATATCTGGTC CTTCAGGCCC TCCTGGGCCT CCGGGCTTCC CTGGGGACCG TGGTCTACCG 
GGTCCTGCCG GCCTCCCAGG AATCCCAGGC ATCGATGGAG CCCGGGGCCT GCCGGGCACA 
GTGATTATGA TGCCGTTCCA TTTTGCAAGC AGCTCGATGA AGGGACCCCC AGTGTCCTTC 
CAGCAGGCCC AGGCCCAGGC AGTATTGCAA CAGGCTCAGC TGTCCATGAA AGGGCCCCCT 
GGTCCAGTAG GGCTCACTGG GCGCCCAGGC CCTGTGGGCC TCCCTGGATA TCCAGGTCTG 
AAAGGTGAAC TGGGAGAAGT GGGGCCACAG GGCCCCCGAG GATTACAGGG CCCTCCTGGG 
CCTCCTGGAC GGGAAGGCAA GACAGGCCGA GCTGGAGCAG ATGGGGCTCG GGGGCTCCCG 
GGAGACACAG GACCTAAGGG TGACAGGGGC TTTGATGGCC TGCCCGGGCT GCCTGGTGAG 
AAGGGCCAAA GGGGTGACTT TGGACGAGTA GGGCAACCTG GTCCCCCAGG AGAGGATGGT 
GTAAAGGGCC TGCAGGGACC TCCAGGGCCC ACTGGCCAGG CTGGAGAGCC GGGTCCCCGA 
GGTCTGATTG GCCCCAGAGG TCTCCCAGGT CCCCTAGGAC GCCCGGGTGT GACAGGGAGT 
GATGGCGCAC CAGGGGCCAA AGGCAACGTG GGTCCTCCTG GAGAACCAGG ACCCCCAGGA 
CAGCAAGGAA ACCACGGCTC CCAGGGAATT CCAGGCCCCC AGGGGCCCAT TGGCACTCCC 
GGGGAAAAGG GTCCCCCTGG AAACCCCGGA ATTCCAGGTG TCCCAGGATC TGAGGGCCCC 
CCGGGCCACC CAGGCCACGA GGGTCCCACA GGAGAAAAAG GGGCTCAGGG CCCACCAGGA 
TCAGCAGGCC CTCGGGGCTA TCCTGGACTT CGTGGTGTGA AGGGTACCTC TGGTAACCGG 
GGTCTCCAAG GCGAGAAAGG AGAAAGGGGA GAGGATGGCT TTCCTGGCTT CAAGGGTGAT 
GAGGGACCAA AAGGCGACCG GGGAAACCCC GGACCCCCAG GTCCCAGAGG AGAGGATGGT 
CCAGAAGGAC AAAAGGGGCC TGGGGGACTG CCTGGTGATG AGGGTCCTCC AGGAGCAGCA 
GGGGAGAAGG GCAAGCTTGG GGTGCCAGGT CTCCCAGGTT ATCCAGGACG CCCAGGACCT 
AAGGGATCTA TTGGATTTCC TGGACCCTTG GGACCACTGG GGGAGAAAGG CAAGCGGGGC 
AAAGCAGGAC AGCCAGGAGA GGAAGGAGAA CGCGGCACAC CGGGCACCCG AGGAGACAGG 
GGACAGCCGG GGGCCACAGG CCAGCCTGGC CCCAAGGGTG ACGTGGGCCA GAATGGGTCT 
CCTGGGCCCC CTGGAGAAAA GGGTCTACCC GGTCTTCAAG GCCCACCAGG ATTCCCCGGA 
CCAAAAGGCC CCCCGGGTCC TCAGGGGAAA GATGGGATAT CTGGGCACCC TGGACAAAGA 
GGAGAATTGG GCTTCCAAGG TCTGACAGGC CCCCCTGGAC CAGCTGGCGT CCTTGGTCCT 
CAGGGAAAGG TAGGGGACGT GGGGCCTCTA GGCGAGAGAG GCCCCCCAGG GCCTCCTGGA 
CCTCCTGGTG AACAAGGTCT GCCAGGCATA GAAGGCAGAG AAGGGGCCAA GGGTGAGCTA 
GGACCCCTGG GGTCCGTCGG GAAGGAGGGG CCACCTGGGC CCAGGGGCTT CCCTGGCCCC 
CAAGGAGCCC CCGGAGACCC AGGACCCATT GGTTTGAAGG GTGACAAAGG TCCCCCAGGC 
CCTGTTGGGG CAAATGGCTC CCCGGGAGAG CGTGGTCCTG TAGGCCCCTC TGGTGGCATT 
GGGCTTCCTG GCCAGAGTGG AGGGCAAGGC CCTATTGGTC CTGCTGGCGA GAAGGGGTCC 
CCGGGAGAAC GGGGTACTCC TGGTCCTACT GGCAAAGATG GTATTCCAGG ACCCCCGGGG 
CTTCAGGGCC CCTCTGGAGC TGCGGGGCCT TCTGGGGAAG AAGGAGACAA GGGGGAAGTA 
GGGATGCCTG GTCACAAAGG AAGCAAAGGG GATAAAGGAG ATGCAGGCCC ACCTGGACCA 
ACAGGAATAA GAGGTCCAGC AGGCCATTCA GGCCTCCCGA AACAACTGTA TATACGATGC 
TGCATTTCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col5a3 ( NM_016919.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_058615.1 Mus musculus collagen, type V, alpha 3 (Col5a3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu10729
Clone ID Related Accession (Same CDS sequence)	NM_016919.3
Accession Version	NM_016919.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-20
Organism	Mus musculus(house mouse)
Product	collagen alpha-3(V) chain isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC166365.4, BY783304.1, BC138417.1, BX511958.1, AI585575.1 and AI482539.1. On Dec 3, 2015 this sequence version replaced NM_016919.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF176645.1, BC138417.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAAGCT GCCGGAGACT GGATCAGCTT CAGGCCGGCC TCTGCCTGCT CCTGGCCTCC 
CTGCAGCTCG TGTCCTGGAC GCTGGCTGCA GAACCTGTGG ACGTACTGGA AGCCTGGGGT 
GTGCATAGAG ACCAGGCTGG GGTGGCTGAA GGGCCTGGCT TCTGCCCCCT GAGGATTCCA 
CAGGGTGACC GAGCATTCAG GGTGGGCAAG TCCAGCCTTC TCAGTGTCCC CACGTGGCAG 
CTCTTCCCAG ATGGGCATTT TCCTGAGAAC TTTTCTGTGC TGCTCACACT GAGGGCCCAG 
CCAGCCAATC AGTCTGTCCT TCTGTCTATT TATGATGAGA AGGGTGTCCG GCAGCTGGGG 
CTGGCACTGG GGCCAGCTCT GGGCCTCCTT GGTGACTCCT TCAGGCCCCT CCCCAAGCAA 
GTCAACATTA TGGATGGCAG GTGGCACCGT GTGGCAGTCA GCATCAGTGG TAACAAGGTG 
ACCCTGGTGG TTGACTGTGA ACCGCAGCCC CCAACATTTG GTCAGGGGCC TCGGTTTATA 
AGTACAGCTG GACTCACTGT GATGGGAACC CAGGACACCA GGGAAGAGTC TTTTGAGGGA 
GACATCCAGG AGCTGCTGTT AATTCCAGAC CCTCAGGCTG CCTTCCAGGC CTGTGAGAGC 
TACCTCCCTG GTTGTGAAAC CCTCGATTCC ACAACCACAG GGGCCCCCAA AGACGATGAA 
CCAGAAACCC CTGCCCCTCG TCGTCGAAAG GGCAAAGGGA AGAAAAAAGG GCGGGGTCGA 
AAGGGCAAGG GAAGAAAGAA AAACAAGGAG ACCTCAGAGC TGAGTCCGAC CCCTGGTGCC 
CCTGAGAACC AGACCTCCCT CCACATCCCT GAGACAGAGA AGACAGTTCC CCACCTGCCT 
CTGACTCCCA CACCTCTGGC CATCACCACC ACTGTCACGA TTGGACAAAA TGCCACAGTC 
TCGCAGGGGT TGGACTCCGG TACTGAAACC GAGCAGACGA CTCCAGAGGT GGACTCTACT 
GAGGAGGGTG AAGGAGGTGG CCCCACCATG GGCCCCAAGT TCCGGGCAGC AGAGCAGTCC 
TTACAGACTG AGTTCCAGAT CTTTCCTGGT GCTGGAGAAA AGGGAGCGAA AGGAGAGCCT 
GCGACAGTAG AGCAGGGACA GCAGTTTGAG GGGCCTGCAG GAGCTCCAGG ACCCCGGGGA 
ATATCTGGTC CTTCAGGCCC TCCTGGGCCT CCGGGCTTCC CTGGGGACCG TGGTCTACCG 
GGTCCTGCCG GCCTCCCAGG AATCCCAGGC ATCGATGGAG CCCGGGGCCT GCCGGGCACA 
GTGATTATGA TGCCGTTCCA TTTTGCAAGC AGCTCGATGA AGGGACCCCC AGTGTCCTTC 
CAGCAGGCCC AGGCCCAGGC AGTATTGCAA CAGGCTCAGC TGTCCATGAA AGGGCCCCCT 
GGTCCAGTAG GGCTCACTGG GCGCCCAGGC CCTGTGGGCC TCCCTGGATA TCCAGGTCTG 
AAAGGTGAAC TGGGAGAAGT GGGGCCACAG GGCCCCCGAG GATTACAGGG CCCTCCTGGG 
CCTCCTGGAC GGGAAGGCAA GACAGGCCGA GCTGGAGCAG ATGGGGCTCG GGGGCTCCCG 
GGAGACACAG GACCTAAGGG TGACAGGGGC TTTGATGGCC TGCCCGGGCT GCCTGGTGAG 
AAGGGCCAAA GGGGTGACTT TGGACGAGTA GGGCAACCTG GTCCCCCAGG AGAGGATGGT 
GTAAAGGGCC TGCAGGGACC TCCAGGGCCC ACTGGCCAGG CTGGAGAGCC GGGTCCCCGA 
GGTCTGATTG GCCCCAGAGG TCTCCCAGGT CCCCTAGGAC GCCCGGGTGT GACAGGGAGT 
GATGGCGCAC CAGGGGCCAA AGGCAACGTG GGTCCTCCTG GAGAACCAGG ACCCCCAGGA 
CAGCAAGGAA ACCACGGCTC CCAGGGAATT CCAGGCCCCC AGGGGCCCAT TGGCACTCCC 
GGGGAAAAGG GTCCCCCTGG AAACCCCGGA ATTCCAGGTG TCCCAGGATC TGAGGGCCCC 
CCGGGCCACC CAGGCCACGA GGGTCCCACA GGAGAAAAAG GGGCTCAGGG CCCACCAGGA 
TCAGCAGGCC CTCGGGGCTA TCCTGGACTT CGTGGTGTGA AGGGTACCTC TGGTAACCGG 
GGTCTCCAAG GCGAGAAAGG AGAAAGGGGA GAGGATGGCT TTCCTGGCTT CAAGGGTGAT 
GAGGGACCAA AAGGCGACCG GGGAAACCCC GGACCCCCAG GTCCCAGAGG AGAGGATGGT 
CCAGAAGGAC AAAAGGGGCC TGGGGGACTG CCTGGTGATG AGGGTCCTCC AGGAGCAGCA 
GGGGAGAAGG GCAAGCTTGG GGTGCCAGGT CTCCCAGGTT ATCCAGGACG CCCAGGACCT 
AAGGGATCTA TTGGATTTCC TGGACCCTTG GGACCACTGG GGGAGAAAGG CAAGCGGGGC 
AAAGCAGGAC AGCCAGGAGA GGAAGGAGAA CGCGGCACAC CGGGCACCCG AGGAGACAGG 
GGACAGCCGG GGGCCACAGG CCAGCCTGGC CCCAAGGGTG ACGTGGGCCA GAATGGGTCT 
CCTGGGCCCC CTGGAGAAAA GGGTCTACCC GGTCTTCAAG GCCCACCAGG ATTCCCCGGA 
CCAAAAGGCC CCCCGGGTCC TCAGGGGAAA GATGGGATAT CTGGGCACCC TGGACAAAGA 
GGAGAATTGG GCTTCCAAGG TCTGACAGGC CCCCCTGGAC CAGCTGGCGT CCTTGGTCCT 
CAGGGAAAGG TAGGGGACGT GGGGCCTCTA GGCGAGAGAG GCCCCCCAGG GCCTCCTGGA 
CCTCCTGGTG AACAAGGTCT GCCAGGCATA GAAGGCAGAG AAGGGGCCAA GGGTGAGCTA 
GGACCCCTGG GGTCCGTCGG GAAGGAGGGG CCACCTGGGC CCAGGGGCTT CCCTGGCCCC 
CAAGGAGCCC CCGGAGACCC AGGACCCATT GGTTTGAAGG GTGACAAAGG TCCCCCAGGC 
CCTGTTGGGG CAAATGGCTC CCCGGGAGAG CGTGGTCCTG TAGGCCCCTC TGGTGGCATT 
GGGCTTCCTG GCCAGAGTGG AGGGCAAGGC CCTATTGGTC CTGCTGGCGA GAAGGGGTCC 
CCGGGAGAAC GGGGTACTCC TGGTCCTACT GGCAAAGATG GTATTCCAGG ACCCCCGGGG 
CTTCAGGGCC CCTCTGGAGC TGCGGGGCCT TCTGGGGAAG AAGGAGACAA GGGGGAAGTA 
GGGATGCCTG GTCACAAAGG AAGCAAAGGG GATAAAGGAG ATGCAGGCCC ACCTGGACCA 
ACAGGAATAA GAGGTCCAGC AGGCCATTCA GGCCTCCCGG GTGCTGATGG CGCTCAGGGT 
CGCCGGGGAC CCCCTGGCCT CTTCGGGCAG AAGGGGGATG ACGGAGTTCG AGGCTTTGTA 
GGTGTAATTG GTCCTCCAGG TCTGCAGGGG CTGCCGGGTC CTCCGGGGGA GAAGGGCGAG 
GTTGGAGACG TAGGATCCAT GGGTCCACAT GGAGCTCCAG GCCCTCGGGG TCCCCCTGGG 
CCCAGTGGAT CAGAGGGCCC CCCAGGTCTG CCTGGAGGAG TAGGACAGCC TGGTGCTGTG 
GGCGAGAAGG GTGAGCCAGG GGATGCTGGA GACGCCGGAC CCCCAGGAAT TCCCGGCATC 
CCTGGGCCCA AAGGTGAAAT TGGTGAAAAG GGGGATTCGG GTCCATCAGG GGCTGCTGGT 
CCCCCAGGCA AGAAAGGACC CCCAGGAGAG GACGGCTCTA AGGGGAACAT GGGTCCCACA 
GGACTCCCTG GAGATCTAGG GCCCCCAGGA GACCCTGGAG TTCCGGGTAT TGATGGCATC 
CCAGGGGAGA AGGGAAATGC TGGTGATATT GGGGGACCGG GGCCACCTGG AGCTTCCGGG 
GAACCTGGTG CCCGTGGCCT CCCTGGCAAG AGGGGTTCCC CTGGCCGCAT GGGTCCAGAA 
GGAAGAGAGG GCGAGAAAGG CGCCAAGGGA GATGCTGGTC CTGATGGACC CCCAGGCAGG 
ACAGGCCCCA TTGGGGCTCG AGGGCCCCCT GGACGAATTG GGCCTGATGG TCTTCCAGGG 
ATCCCTGGTC CTGTGGGTGA ACCAGGTCTC CTGGGACCTC CTGGGCTAAT CGGCCCTCCA 
GGGCCCCTGG GCCCACCTGG CCTCCCTGGC CTGAAGGGAG ATGCTGGCCC CAAGGGGGAG 
AAGGGCCACA TTGGGCTAAT AGGCCTCATT GGTCCCCCAG GGGAGGCCGG TGAGAAAGGC 
GATCAGGGGT TGCCAGGTGT GCAGGGCCCC CCAGGCCTTC AGGGAGACCC TGGTCTCCCT 
GGTCCTGTTG GCTCGTTAGG TCACCCTGGG CCCCCAGGTG TGGTGGGCCC TCTGGGACAG 
AAGGGCTCCA AAGGGTCCCC GGGATCTCTT GGTCCTCGTG GAGACCCTGG ACCAGCGGGT 
CCTCCTGGTC CCCCGGGTTC TCCGGCTGAG GTGCATGGCC TGCGCAGGCG CCGATCTGTG 
ACGGACACCC TGGAAGGTGG CCTGGAGGAG GTGATGGCCT CACTGAATTC ACTGAGCTTG 
GAGCTGCAGC AGTTGCAGAG ACCTCTGGGC ACAGCCGAGA GCCCAGGCCT CATGTGCCGA 
GAGCTTCACC GCGACCACCC ACACCTGCCC GATGGAGAGT ACTGGATTGA CCCCAATCAG 
GGCTGTGCAC GTGACGCCTT CAAGGTTTTC TGCAACTTCA CGGCAGGAGG TGAGACCTGT 
CTCTATCCAG ACAAGAAGTT TGAGACGGTG AAACTGGCCT CGTGGTCCCG AGAGAAGCCT 
GGAGGCTGGT ACAGCACCTT CCGCCGAGGG AAGAAGTTCT CCTATGTGGA TGCTGATGGC 
TCCCCGGTGA ATGTGGTCCA GTTGACCTTC CTGAAGTTGT TGAGTGCTGC AGCCCATCAG 
AGGTTCACTT ACATCTGCCA GAACTCGGTG GCATGGCTGG ATGAAGCTGC GGGTGACCAC 
AGGCACTCCA TCCGCTTCCA AGGGACCAAC TGGGAAGAGT TGTCCTTCAA CCAGACAACA 
GCAGCTACCA TCAAGGTCTC CCATGATGGC TGTCGGGTCC GGAAGGGACA GGCGAAGACC 
CTCTTTGAAT TCAGCTCTTC TGTGGGTTTC CTGCCTCTGT GGGATGTGGC TGCCTCTGAC 
TTTGGTCAGA CGAACCAAAA GTTTGGGTTT GAACTCGGCT CCATCTGCTT TAGCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058615.1
CDS	115..5334
Translation	MRSCRRLDQLQAGLCLLLASLQLVSWTLAAEPVDVLEAWGVHRDQAGVAEGPGFCPLRIPQGDRAFRVGKSSLLSVPTWQLFPDGHFPENFSVLLTLRAQPANQSVLLSIYDEKGVRQLGLALGPALGLLGDSFRPLPKQVNIMDGRWHRVAVSISGNKVTLVVDCEPQPPTFGQGPRFISTAGLTVMGTQDTREESFEGDIQELLLIPDPQAAFQACESYLPGCETLDSTTTGAPKDDEPETPAPRRRKGKGKKKGRGRKGKGRKKNKETSELSPTPGAPENQTSLHIPETEKTVPHLPLTPTPLAITTTVTIGQNATVSQGLDSGTETEQTTPEVDSTEEGEGGGPTMGPKFRAAEQSLQTEFQIFPGAGEKGAKGEPATVEQGQQFEGPAGAPGPRGISGPSGPPGPPGFPGDRGLPGPAGLPGIPGIDGARGLPGTVIMMPFHFASSSMKGPPVSFQQAQAQAVLQQAQLSMKGPPGPVGLTGRPGPVGLPGYPGLKGELGEVGPQGPRGLQGPPGPPGREGKTGRAGADGARGLPGDTGPKGDRGFDGLPGLPGEKGQRGDFGRVGQPGPPGEDGVKGLQGPPGPTGQAGEPGPRGLIGPRGLPGPLGRPGVTGSDGAPGAKGNVGPPGEPGPPGQQGNHGSQGIPGPQGPIGTPGEKGPPGNPGIPGVPGSEGPPGHPGHEGPTGEKGAQGPPGSAGPRGYPGLRGVKGTSGNRGLQGEKGERGEDGFPGFKGDEGPKGDRGNPGPPGPRGEDGPEGQKGPGGLPGDEGPPGAAGEKGKLGVPGLPGYPGRPGPKGSIGFPGPLGPLGEKGKRGKAGQPGEEGERGTPGTRGDRGQPGATGQPGPKGDVGQNGSPGPPGEKGLPGLQGPPGFPGPKGPPGPQGKDGISGHPGQRGELGFQGLTGPPGPAGVLGPQGKVGDVGPLGERGPPGPPGPPGEQGLPGIEGREGAKGELGPLGSVGKEGPPGPRGFPGPQGAPGDPGPIGLKGDKGPPGPVGANGSPGERGPVGPSGGIGLPGQSGGQGPIGPAGEKGSPGERGTPGPTGKDGIPGPPGLQGPSGAAGPSGEEGDKGEVGMPGHKGSKGDKGDAGPPGPTGIRGPAGHSGLPGADGAQGRRGPPGLFGQKGDDGVRGFVGVIGPPGLQGLPGPPGEKGEVGDVGSMGPHGAPGPRGPPGPSGSEGPPGLPGGVGQPGAVGEKGEPGDAGDAGPPGIPGIPGPKGEIGEKGDSGPSGAAGPPGKKGPPGEDGSKGNMGPTGLPGDLGPPGDPGVPGIDGIPGEKGNAGDIGGPGPPGASGEPGARGLPGKRGSPGRMGPEGREGEKGAKGDAGPDGPPGRTGPIGARGPPGRIGPDGLPGIPGPVGEPGLLGPPGLIGPPGPLGPPGLPGLKGDAGPKGEKGHIGLIGLIGPPGEAGEKGDQGLPGVQGPPGLQGDPGLPGPVGSLGHPGPPGVVGPLGQKGSKGSPGSLGPRGDPGPAGPPGPPGSPAEVHGLRRRRSVTDTLEGGLEEVMASLNSLSLELQQLQRPLGTAESPGLMCRELHRDHPHLPDGEYWIDPNQGCARDAFKVFCNFTAGGETCLYPDKKFETVKLASWSREKPGGWYSTFRRGKKFSYVDADGSPVNVVQLTFLKLLSAAAHQRFTYICQNSVAWLDEAAGDHRHSIRFQGTNWEELSFNQTTAATIKVSHDGCRVRKGQAKTLFEFSSSVGFLPLWDVAASDFGQTNQKFGFELGSICFSS

Target ORF information:

RefSeq Version	NM_016919.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type V, alpha 3 (Col5a3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016919.3

ORF Insert Sequence:

ATGAGAAGCT GCCGGAGACT GGATCAGCTT CAGGCCGGCC TCTGCCTGCT CCTGGCCTCC 
CTGCAGCTCG TGTCCTGGAC GCTGGCTGCA GAACCTGTGG ACGTACTGGA AGCCTGGGGT 
GTGCATAGAG ACCAGGCTGG GGTGGCTGAA GGGCCTGGCT TCTGCCCCCT GAGGATTCCA 
CAGGGTGACC GAGCATTCAG GGTGGGCAAG TCCAGCCTTC TCAGTGTCCC CACGTGGCAG 
CTCTTCCCAG ATGGGCATTT TCCTGAGAAC TTTTCTGTGC TGCTCACACT GAGGGCCCAG 
CCAGCCAATC AGTCTGTCCT TCTGTCTATT TATGATGAGA AGGGTGTCCG GCAGCTGGGG 
CTGGCACTGG GGCCAGCTCT GGGCCTCCTT GGTGACTCCT TCAGGCCCCT CCCCAAGCAA 
GTCAACATTA TGGATGGCAG GTGGCACCGT GTGGCAGTCA GCATCAGTGG TAACAAGGTG 
ACCCTGGTGG TTGACTGTGA ACCGCAGCCC CCAACATTTG GTCAGGGGCC TCGGTTTATA 
AGTACAGCTG GACTCACTGT GATGGGAACC CAGGACACCA GGGAAGAGTC TTTTGAGGGA 
GACATCCAGG AGCTGCTGTT AATTCCAGAC CCTCAGGCTG CCTTCCAGGC CTGTGAGAGC 
TACCTCCCTG GTTGTGAAAC CCTCGATTCC ACAACCACAG GGGCCCCCAA AGACGATGAA 
CCAGAAACCC CTGCCCCTCG TCGTCGAAAG GGCAAAGGGA AGAAAAAAGG GCGGGGTCGA 
AAGGGCAAGG GAAGAAAGAA AAACAAGGAG ACCTCAGAGC TGAGTCCGAC CCCTGGTGCC 
CCTGAGAACC AGACCTCCCT CCACATCCCT GAGACAGAGA AGACAGTTCC CCACCTGCCT 
CTGACTCCCA CACCTCTGGC CATCACCACC ACTGTCACGA TTGGACAAAA TGCCACAGTC 
TCGCAGGGGT TGGACTCCGG TACTGAAACC GAGCAGACGA CTCCAGAGGT GGACTCTACT 
GAGGAGGGTG AAGGAGGTGG CCCCACCATG GGCCCCAAGT TCCGGGCAGC AGAGCAGTCC 
TTACAGACTG AGTTCCAGAT CTTTCCTGGT GCTGGAGAAA AGGGAGCGAA AGGAGAGCCT 
GCGACAGTAG AGCAGGGACA GCAGTTTGAG GGGCCTGCAG GAGCTCCAGG ACCCCGGGGA 
ATATCTGGTC CTTCAGGCCC TCCTGGGCCT CCGGGCTTCC CTGGGGACCG TGGTCTACCG 
GGTCCTGCCG GCCTCCCAGG AATCCCAGGC ATCGATGGAG CCCGGGGCCT GCCGGGCACA 
GTGATTATGA TGCCGTTCCA TTTTGCAAGC AGCTCGATGA AGGGACCCCC AGTGTCCTTC 
CAGCAGGCCC AGGCCCAGGC AGTATTGCAA CAGGCTCAGC TGTCCATGAA AGGGCCCCCT 
GGTCCAGTAG GGCTCACTGG GCGCCCAGGC CCTGTGGGCC TCCCTGGATA TCCAGGTCTG 
AAAGGTGAAC TGGGAGAAGT GGGGCCACAG GGCCCCCGAG GATTACAGGG CCCTCCTGGG 
CCTCCTGGAC GGGAAGGCAA GACAGGCCGA GCTGGAGCAG ATGGGGCTCG GGGGCTCCCG 
GGAGACACAG GACCTAAGGG TGACAGGGGC TTTGATGGCC TGCCCGGGCT GCCTGGTGAG 
AAGGGCCAAA GGGGTGACTT TGGACGAGTA GGGCAACCTG GTCCCCCAGG AGAGGATGGT 
GTAAAGGGCC TGCAGGGACC TCCAGGGCCC ACTGGCCAGG CTGGAGAGCC GGGTCCCCGA 
GGTCTGATTG GCCCCAGAGG TCTCCCAGGT CCCCTAGGAC GCCCGGGTGT GACAGGGAGT 
GATGGCGCAC CAGGGGCCAA AGGCAACGTG GGTCCTCCTG GAGAACCAGG ACCCCCAGGA 
CAGCAAGGAA ACCACGGCTC CCAGGGAATT CCAGGCCCCC AGGGGCCCAT TGGCACTCCC 
GGGGAAAAGG GTCCCCCTGG AAACCCCGGA ATTCCAGGTG TCCCAGGATC TGAGGGCCCC 
CCGGGCCACC CAGGCCACGA GGGTCCCACA GGAGAAAAAG GGGCTCAGGG CCCACCAGGA 
TCAGCAGGCC CTCGGGGCTA TCCTGGACTT CGTGGTGTGA AGGGTACCTC TGGTAACCGG 
GGTCTCCAAG GCGAGAAAGG AGAAAGGGGA GAGGATGGCT TTCCTGGCTT CAAGGGTGAT 
GAGGGACCAA AAGGCGACCG GGGAAACCCC GGACCCCCAG GTCCCAGAGG AGAGGATGGT 
CCAGAAGGAC AAAAGGGGCC TGGGGGACTG CCTGGTGATG AGGGTCCTCC AGGAGCAGCA 
GGGGAGAAGG GCAAGCTTGG GGTGCCAGGT CTCCCAGGTT ATCCAGGACG CCCAGGACCT 
AAGGGATCTA TTGGATTTCC TGGACCCTTG GGACCACTGG GGGAGAAAGG CAAGCGGGGC 
AAAGCAGGAC AGCCAGGAGA GGAAGGAGAA CGCGGCACAC CGGGCACCCG AGGAGACAGG 
GGACAGCCGG GGGCCACAGG CCAGCCTGGC CCCAAGGGTG ACGTGGGCCA GAATGGGTCT 
CCTGGGCCCC CTGGAGAAAA GGGTCTACCC GGTCTTCAAG GCCCACCAGG ATTCCCCGGA 
CCAAAAGGCC CCCCGGGTCC TCAGGGGAAA GATGGGATAT CTGGGCACCC TGGACAAAGA 
GGAGAATTGG GCTTCCAAGG TCTGACAGGC CCCCCTGGAC CAGCTGGCGT CCTTGGTCCT 
CAGGGAAAGG TAGGGGACGT GGGGCCTCTA GGCGAGAGAG GCCCCCCAGG GCCTCCTGGA 
CCTCCTGGTG AACAAGGTCT GCCAGGCATA GAAGGCAGAG AAGGGGCCAA GGGTGAGCTA 
GGACCCCTGG GGTCCGTCGG GAAGGAGGGG CCACCTGGGC CCAGGGGCTT CCCTGGCCCC 
CAAGGAGCCC CCGGAGACCC AGGACCCATT GGTTTGAAGG GTGACAAAGG TCCCCCAGGC 
CCTGTTGGGG CAAATGGCTC CCCGGGAGAG CGTGGTCCTG TAGGCCCCTC TGGTGGCATT 
GGGCTTCCTG GCCAGAGTGG AGGGCAAGGC CCTATTGGTC CTGCTGGCGA GAAGGGGTCC 
CCGGGAGAAC GGGGTACTCC TGGTCCTACT GGCAAAGATG GTATTCCAGG ACCCCCGGGG 
CTTCAGGGCC CCTCTGGAGC TGCGGGGCCT TCTGGGGAAG AAGGAGACAA GGGGGAAGTA 
GGGATGCCTG GTCACAAAGG AAGCAAAGGG GATAAAGGAG ATGCAGGCCC ACCTGGACCA 
ACAGGAATAA GAGGTCCAGC AGGCCATTCA GGCCTCCCGG GTGCTGATGG CGCTCAGGGT 
CGCCGGGGAC CCCCTGGCCT CTTCGGGCAG AAGGGGGATG ACGGAGTTCG AGGCTTTGTA 
GGTGTAATTG GTCCTCCAGG TCTGCAGGGG CTGCCGGGTC CTCCGGGGGA GAAGGGCGAG 
GTTGGAGACG TAGGATCCAT GGGTCCACAT GGAGCTCCAG GCCCTCGGGG TCCCCCTGGG 
CCCAGTGGAT CAGAGGGCCC CCCAGGTCTG CCTGGAGGAG TAGGACAGCC TGGTGCTGTG 
GGCGAGAAGG GTGAGCCAGG GGATGCTGGA GACGCCGGAC CCCCAGGAAT TCCCGGCATC 
CCTGGGCCCA AAGGTGAAAT TGGTGAAAAG GGGGATTCGG GTCCATCAGG GGCTGCTGGT 
CCCCCAGGCA AGAAAGGACC CCCAGGAGAG GACGGCTCTA AGGGGAACAT GGGTCCCACA 
GGACTCCCTG GAGATCTAGG GCCCCCAGGA GACCCTGGAG TTCCGGGTAT TGATGGCATC 
CCAGGGGAGA AGGGAAATGC TGGTGATATT GGGGGACCGG GGCCACCTGG AGCTTCCGGG 
GAACCTGGTG CCCGTGGCCT CCCTGGCAAG AGGGGTTCCC CTGGCCGCAT GGGTCCAGAA 
GGAAGAGAGG GCGAGAAAGG CGCCAAGGGA GATGCTGGTC CTGATGGACC CCCAGGCAGG 
ACAGGCCCCA TTGGGGCTCG AGGGCCCCCT GGACGAATTG GGCCTGATGG TCTTCCAGGG 
ATCCCTGGTC CTGTGGGTGA ACCAGGTCTC CTGGGACCTC CTGGGCTAAT CGGCCCTCCA 
GGGCCCCTGG GCCCACCTGG CCTCCCTGGC CTGAAGGGAG ATGCTGGCCC CAAGGGGGAG 
AAGGGCCACA TTGGGCTAAT AGGCCTCATT GGTCCCCCAG GGGAGGCCGG TGAGAAAGGC 
GATCAGGGGT TGCCAGGTGT GCAGGGCCCC CCAGGCCTTC AGGGAGACCC TGGTCTCCCT 
GGTCCTGTTG GCTCGTTAGG TCACCCTGGG CCCCCAGGTG TGGTGGGCCC TCTGGGACAG 
AAGGGCTCCA AAGGGTCCCC GGGATCTCTT GGTCCTCGTG GAGACCCTGG ACCAGCGGGT 
CCTCCTGGTC CCCCGGGTTC TCCGGCTGAG GTGCATGGCC TGCGCAGGCG CCGATCTGTG 
ACGGACACCC TGGAAGGTGG CCTGGAGGAG GTGATGGCCT CACTGAATTC ACTGAGCTTG 
GAGCTGCAGC AGTTGCAGAG ACCTCTGGGC ACAGCCGAGA GCCCAGGCCT CATGTGCCGA 
GAGCTTCACC GCGACCACCC ACACCTGCCC GATGGAGAGT ACTGGATTGA CCCCAATCAG 
GGCTGTGCAC GTGACGCCTT CAAGGTTTTC TGCAACTTCA CGGCAGGAGG TGAGACCTGT 
CTCTATCCAG ACAAGAAGTT TGAGACGGTG AAACTGGCCT CGTGGTCCCG AGAGAAGCCT 
GGAGGCTGGT ACAGCACCTT CCGCCGAGGG AAGAAGTTCT CCTATGTGGA TGCTGATGGC 
TCCCCGGTGA ATGTGGTCCA GTTGACCTTC CTGAAGTTGT TGAGTGCTGC AGCCCATCAG 
AGGTTCACTT ACATCTGCCA GAACTCGGTG GCATGGCTGG ATGAAGCTGC GGGTGACCAC 
AGGCACTCCA TCCGCTTCCA AGGGACCAAC TGGGAAGAGT TGTCCTTCAA CCAGACAACA 
GCAGCTACCA TCAAGGTCTC CCATGATGGC TGTCGGGTCC GGAAGGGACA GGCGAAGACC 
CTCTTTGAAT TCAGCTCTTC TGTGGGTTTC CTGCCTCTGT GGGATGTGGC TGCCTCTGAC 
TTTGGTCAGA CGAACCAAAA GTTTGGGTTT GAACTCGGCT CCATCTGCTT TAGCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col5a3 ( NM_001317388.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001304317.1 Mus musculus collagen, type V, alpha 3 (Col5a3), transcript variant 2, mRNA.

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CloneID	OMu42318
Clone ID Related Accession (Same CDS sequence)	NM_001317388.1
Accession Version	NM_001317388.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-20
Organism	Mus musculus(house mouse)
Product	collagen alpha-3(V) chain isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC166365.4, BY783304.1, BC138417.1, BX511958.1, AI585575.1 and AI482539.1. On Dec 3, 2015 this sequence version replaced XM_006510487.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC145269.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAAGCT GCCGGAGACT GGATCAGCTT CAGGCCGGCC TCTGCCTGCT CCTGGCCTCC 
CTGCAGCTCG TGTCCTGGAC GCTGGCTGAA CCTGTGGACG TACTGGAAGC CTGGGGTGTG 
CATAGAGACC AGGCTGGGGT GGCTGAAGGG CCTGGCTTCT GCCCCCTGAG GATTCCACAG 
GGTGACCGAG CATTCAGGGT GGGCAAGTCC AGCCTTCTCA GTGTCCCCAC GTGGCAGCTC 
TTCCCAGATG GGCATTTTCC TGAGAACTTT TCTGTGCTGC TCACACTGAG GGCCCAGCCA 
GCCAATCAGT CTGTCCTTCT GTCTATTTAT GATGAGAAGG GTGTCCGGCA GCTGGGGCTG 
GCACTGGGGC CAGCTCTGGG CCTCCTTGGT GACTCCTTCA GGCCCCTCCC CAAGCAAGTC 
AACATTATGG ATGGCAGGTG GCACCGTGTG GCAGTCAGCA TCAGTGGTAA CAAGGTGACC 
CTGGTGGTTG ACTGTGAACC GCAGCCCCCA ACATTTGGTC AGGGGCCTCG GTTTATAAGT 
ACAGCTGGAC TCACTGTGAT GGGAACCCAG GACACCAGGG AAGAGTCTTT TGAGGGAGAC 
ATCCAGGAGC TGCTGTTAAT TCCAGACCCT CAGGCTGCCT TCCAGGCCTG TGAGAGCTAC 
CTCCCTGGTT GTGAAACCCT CGATTCCACA ACCACAGGGG CCCCCAAAGA CGATGAACCA 
GAAACCCCTG CCCCTCGTCG TCGAAAGGGC AAAGGGAAGA AAAAAGGGCG GGGTCGAAAG 
GGCAAGGGAA GAAAGAAAAA CAAGGAGACC TCAGAGCTGA GTCCGACCCC TGGTGCCCCT 
GAGAACCAGA CCTCCCTCCA CATCCCTGAG ACAGAGAAGA CAGTTCCCCA CCTGCCTCTG 
ACTCCCACAC CTCTGGCCAT CACCACCACT GTCACGATTG GACAAAATGC CACAGTCTCG 
CAGGGGTTGG ACTCCGGTAC TGAAACCGAG CAGACGACTC CAGAGGTGGA CTCTACTGAG 
GAGGGTGAAG GAGGTGGCCC CACCATGGGC CCCAAGTTCC GGGCAGCAGA GCAGTCCTTA 
CAGACTGAGT TCCAGATCTT TCCTGGTGCT GGAGAAAAGG GAGCGAAAGG AGAGCCTGCG 
ACAGTAGAGC AGGGACAGCA GTTTGAGGGG CCTGCAGGAG CTCCAGGACC CCGGGGAATA 
TCTGGTCCTT CAGGCCCTCC TGGGCCTCCG GGCTTCCCTG GGGACCGTGG TCTACCGGGT 
CCTGCCGGCC TCCCAGGAAT CCCAGGCATC GATGGAGCCC GGGGCCTGCC GGGCACAGTG 
ATTATGATGC CGTTCCATTT TGCAAGCAGC TCGATGAAGG GACCCCCAGT GTCCTTCCAG 
CAGGCCCAGG CCCAGGCAGT ATTGCAACAG GCTCAGCTGT CCATGAAAGG GCCCCCTGGT 
CCAGTAGGGC TCACTGGGCG CCCAGGCCCT GTGGGCCTCC CTGGATATCC AGGTCTGAAA 
GGTGAACTGG GAGAAGTGGG GCCACAGGGC CCCCGAGGAT TACAGGGCCC TCCTGGGCCT 
CCTGGACGGG AAGGCAAGAC AGGCCGAGCT GGAGCAGATG GGGCTCGGGG GCTCCCGGGA 
GACACAGGAC CTAAGGGTGA CAGGGGCTTT GATGGCCTGC CCGGGCTGCC TGGTGAGAAG 
GGCCAAAGGG GTGACTTTGG ACGAGTAGGG CAACCTGGTC CCCCAGGAGA GGATGGTGTA 
AAGGGCCTGC AGGGACCTCC AGGGCCCACT GGCCAGGCTG GAGAGCCGGG TCCCCGAGGT 
CTGATTGGCC CCAGAGGTCT CCCAGGTCCC CTAGGACGCC CGGGTGTGAC AGGGAGTGAT 
GGCGCACCAG GGGCCAAAGG CAACGTGGGT CCTCCTGGAG AACCAGGACC CCCAGGACAG 
CAAGGAAACC ACGGCTCCCA GGGAATTCCA GGCCCCCAGG GGCCCATTGG CACTCCCGGG 
GAAAAGGGTC CCCCTGGAAA CCCCGGAATT CCAGGTGTCC CAGGATCTGA GGGCCCCCCG 
GGCCACCCAG GCCACGAGGG TCCCACAGGA GAAAAAGGGG CTCAGGGCCC ACCAGGATCA 
GCAGGCCCTC GGGGCTATCC TGGACTTCGT GGTGTGAAGG GTACCTCTGG TAACCGGGGT 
CTCCAAGGCG AGAAAGGAGA AAGGGGAGAG GATGGCTTTC CTGGCTTCAA GGGTGATGAG 
GGACCAAAAG GCGACCGGGG AAACCCCGGA CCCCCAGGTC CCAGAGGAGA GGATGGTCCA 
GAAGGACAAA AGGGGCCTGG GGGACTGCCT GGTGATGAGG GTCCTCCAGG AGCAGCAGGG 
GAGAAGGGCA AGCTTGGGGT GCCAGGTCTC CCAGGTTATC CAGGACGCCC AGGACCTAAG 
GGATCTATTG GATTTCCTGG ACCCTTGGGA CCACTGGGGG AGAAAGGCAA GCGGGGCAAA 
GCAGGACAGC CAGGAGAGGA AGGAGAACGC GGCACACCGG GCACCCGAGG AGACAGGGGA 
CAGCCGGGGG CCACAGGCCA GCCTGGCCCC AAGGGTGACG TGGGCCAGAA TGGGTCTCCT 
GGGCCCCCTG GAGAAAAGGG TCTACCCGGT CTTCAAGGCC CACCAGGATT CCCCGGACCA 
AAAGGCCCCC CGGGTCCTCA GGGGAAAGAT GGGATATCTG GGCACCCTGG ACAAAGAGGA 
GAATTGGGCT TCCAAGGTCT GACAGGCCCC CCTGGACCAG CTGGCGTCCT TGGTCCTCAG 
GGAAAGGTAG GGGACGTGGG GCCTCTAGGC GAGAGAGGCC CCCCAGGGCC TCCTGGACCT 
CCTGGTGAAC AAGGTCTGCC AGGCATAGAA GGCAGAGAAG GGGCCAAGGG TGAGCTAGGA 
CCCCTGGGGT CCGTCGGGAA GGAGGGGCCA CCTGGGCCCA GGGGCTTCCC TGGCCCCCAA 
GGAGCCCCCG GAGACCCAGG ACCCATTGGT TTGAAGGGTG ACAAAGGTCC CCCAGGCCCT 
GTTGGGGCAA ATGGCTCCCC GGGAGAGCGT GGTCCTGTAG GCCCCTCTGG TGGCATTGGG 
CTTCCTGGCC AGAGTGGAGG GCAAGGCCCT ATTGGTCCTG CTGGCGAGAA GGGGTCCCCG 
GGAGAACGGG GTACTCCTGG TCCTACTGGC AAAGATGGTA TTCCAGGACC CCCGGGGCTT 
CAGGGCCCCT CTGGAGCTGC GGGGCCTTCT GGGGAAGAAG GAGACAAGGG GGAAGTAGGG 
ATGCCTGGTC ACAAAGGAAG CAAAGGGGAT AAAGGAGATG CAGGCCCACC TGGACCAACA 
GGAATAAGAG GTCCAGCAGG CCATTCAGGC CTCCCGGGTG CTGATGGCGC TCAGGGTCGC 
CGGGGACCCC CTGGCCTCTT CGGGCAGAAG GGGGATGACG GAGTTCGAGG CTTTGTAGGT 
GTAATTGGTC CTCCAGGTCT GCAGGGGCTG CCGGGTCCTC CGGGGGAGAA GGGCGAGGTT 
GGAGACGTAG GATCCATGGG TCCACATGGA GCTCCAGGCC CTCGGGGTCC CCCTGGGCCC 
AGTGGATCAG AGGGCCCCCC AGGTCTGCCT GGAGGAGTAG GACAGCCTGG TGCTGTGGGC 
GAGAAGGGTG AGCCAGGGGA TGCTGGAGAC GCCGGACCCC CAGGAATTCC CGGCATCCCT 
GGGCCCAAAG GTGAAATTGG TGAAAAGGGG GATTCGGGTC CATCAGGGGC TGCTGGTCCC 
CCAGGCAAGA AAGGACCCCC AGGAGAGGAC GGCTCTAAGG GGAACATGGG TCCCACAGGA 
CTCCCTGGAG ATCTAGGGCC CCCAGGAGAC CCTGGAGTTC CGGGTATTGA TGGCATCCCA 
GGGGAGAAGG GAAATGCTGG TGATATTGGG GGACCGGGGC CACCTGGAGC TTCCGGGGAA 
CCTGGTGCCC GTGGCCTCCC TGGCAAGAGG GGTTCCCCTG GCCGCATGGG TCCAGAAGGA 
AGAGAGGGCG AGAAAGGCGC CAAGGGAGAT GCTGGTCCTG ATGGACCCCC AGGCAGGACA 
GGCCCCATTG GGGCTCGAGG GCCCCCTGGA CGAATTGGGC CTGATGGTCT TCCAGGGATC 
CCTGGTCCTG TGGGTGAACC AGGTCTCCTG GGACCTCCTG GGCTAATCGG CCCTCCAGGG 
CCCCTGGGCC CACCTGGCCT CCCTGGCCTG AAGGGAGATG CTGGCCCCAA GGGGGAGAAG 
GGCCACATTG GGCTAATAGG CCTCATTGGT CCCCCAGGGG AGGCCGGTGA GAAAGGCGAT 
CAGGGGTTGC CAGGTGTGCA GGGCCCCCCA GGCCTTCAGG GAGACCCTGG TCTCCCTGGT 
CCTGTTGGCT CGTTAGGTCA CCCTGGGCCC CCAGGTGTGG TGGGCCCTCT GGGACAGAAG 
GGCTCCAAAG GGTCCCCGGG ATCTCTTGGT CCTCGTGGAG ACCCTGGACC AGCGGGTCCT 
CCTGGTCCCC CGGGTTCTCC GGCTGAGGTG CATGGCCTGC GCAGGCGCCG ATCTGTGACG 
GACACCCTGG AAGGTGGCCT GGAGGAGGTG ATGGCCTCAC TGAATTCACT GAGCTTGGAG 
CTGCAGCAGT TGCAGAGACC TCTGGGCACA GCCGAGAGCC CAGGCCTCAT GTGCCGAGAG 
CTTCACCGCG ACCACCCACA CCTGCCCGAT GGAGAGTACT GGATTGACCC CAATCAGGGC 
TGTGCACGTG ACGCCTTCAA GGTTTTCTGC AACTTCACGG CAGGAGGTGA GACCTGTCTC 
TATCCAGACA AGAAGTTTGA GACGGTGAAA CTGGCCTCGT GGTCCCGAGA GAAGCCTGGA 
GGCTGGTACA GCACCTTCCG CCGAGGGAAG AAGTTCTCCT ATGTGGATGC TGATGGCTCC 
CCGGTGAATG TGGTCCAGTT GACCTTCCTG AAGTTGTTGA GTGCTGCAGC CCATCAGAGG 
TTCACTTACA TCTGCCAGAA CTCGGTGGCA TGGCTGGATG AAGCTGCGGG TGACCACAGG 
CACTCCATCC GCTTCCAAGG GACCAACTGG GAAGAGTTGT CCTTCAACCA GACAACAGCA 
GCTACCATCA AGGTCTCCCA TGATGGCTGT CGGGTCCGGA AGGGACAGGC GAAGACCCTC 
TTTGAATTCA GCTCTTCTGT GGGTTTCCTG CCTCTGTGGG ATGTGGCTGC CTCTGACTTT 
GGTCAGACGA ACCAAAAGTT TGGGTTTGAA CTCGGCTCCA TCTGCTTTAG CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304317.1
CDS	115..5331
Translation	MRSCRRLDQLQAGLCLLLASLQLVSWTLAEPVDVLEAWGVHRDQAGVAEGPGFCPLRIPQGDRAFRVGKSSLLSVPTWQLFPDGHFPENFSVLLTLRAQPANQSVLLSIYDEKGVRQLGLALGPALGLLGDSFRPLPKQVNIMDGRWHRVAVSISGNKVTLVVDCEPQPPTFGQGPRFISTAGLTVMGTQDTREESFEGDIQELLLIPDPQAAFQACESYLPGCETLDSTTTGAPKDDEPETPAPRRRKGKGKKKGRGRKGKGRKKNKETSELSPTPGAPENQTSLHIPETEKTVPHLPLTPTPLAITTTVTIGQNATVSQGLDSGTETEQTTPEVDSTEEGEGGGPTMGPKFRAAEQSLQTEFQIFPGAGEKGAKGEPATVEQGQQFEGPAGAPGPRGISGPSGPPGPPGFPGDRGLPGPAGLPGIPGIDGARGLPGTVIMMPFHFASSSMKGPPVSFQQAQAQAVLQQAQLSMKGPPGPVGLTGRPGPVGLPGYPGLKGELGEVGPQGPRGLQGPPGPPGREGKTGRAGADGARGLPGDTGPKGDRGFDGLPGLPGEKGQRGDFGRVGQPGPPGEDGVKGLQGPPGPTGQAGEPGPRGLIGPRGLPGPLGRPGVTGSDGAPGAKGNVGPPGEPGPPGQQGNHGSQGIPGPQGPIGTPGEKGPPGNPGIPGVPGSEGPPGHPGHEGPTGEKGAQGPPGSAGPRGYPGLRGVKGTSGNRGLQGEKGERGEDGFPGFKGDEGPKGDRGNPGPPGPRGEDGPEGQKGPGGLPGDEGPPGAAGEKGKLGVPGLPGYPGRPGPKGSIGFPGPLGPLGEKGKRGKAGQPGEEGERGTPGTRGDRGQPGATGQPGPKGDVGQNGSPGPPGEKGLPGLQGPPGFPGPKGPPGPQGKDGISGHPGQRGELGFQGLTGPPGPAGVLGPQGKVGDVGPLGERGPPGPPGPPGEQGLPGIEGREGAKGELGPLGSVGKEGPPGPRGFPGPQGAPGDPGPIGLKGDKGPPGPVGANGSPGERGPVGPSGGIGLPGQSGGQGPIGPAGEKGSPGERGTPGPTGKDGIPGPPGLQGPSGAAGPSGEEGDKGEVGMPGHKGSKGDKGDAGPPGPTGIRGPAGHSGLPGADGAQGRRGPPGLFGQKGDDGVRGFVGVIGPPGLQGLPGPPGEKGEVGDVGSMGPHGAPGPRGPPGPSGSEGPPGLPGGVGQPGAVGEKGEPGDAGDAGPPGIPGIPGPKGEIGEKGDSGPSGAAGPPGKKGPPGEDGSKGNMGPTGLPGDLGPPGDPGVPGIDGIPGEKGNAGDIGGPGPPGASGEPGARGLPGKRGSPGRMGPEGREGEKGAKGDAGPDGPPGRTGPIGARGPPGRIGPDGLPGIPGPVGEPGLLGPPGLIGPPGPLGPPGLPGLKGDAGPKGEKGHIGLIGLIGPPGEAGEKGDQGLPGVQGPPGLQGDPGLPGPVGSLGHPGPPGVVGPLGQKGSKGSPGSLGPRGDPGPAGPPGPPGSPAEVHGLRRRRSVTDTLEGGLEEVMASLNSLSLELQQLQRPLGTAESPGLMCRELHRDHPHLPDGEYWIDPNQGCARDAFKVFCNFTAGGETCLYPDKKFETVKLASWSREKPGGWYSTFRRGKKFSYVDADGSPVNVVQLTFLKLLSAAAHQRFTYICQNSVAWLDEAAGDHRHSIRFQGTNWEELSFNQTTAATIKVSHDGCRVRKGQAKTLFEFSSSVGFLPLWDVAASDFGQTNQKFGFELGSICFSS

Target ORF information:

RefSeq Version	NM_001317388.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type V, alpha 3 (Col5a3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317388.1

ORF Insert Sequence:

ATGAGAAGCT GCCGGAGACT GGATCAGCTT CAGGCCGGCC TCTGCCTGCT CCTGGCCTCC 
CTGCAGCTCG TGTCCTGGAC GCTGGCTGAA CCTGTGGACG TACTGGAAGC CTGGGGTGTG 
CATAGAGACC AGGCTGGGGT GGCTGAAGGG CCTGGCTTCT GCCCCCTGAG GATTCCACAG 
GGTGACCGAG CATTCAGGGT GGGCAAGTCC AGCCTTCTCA GTGTCCCCAC GTGGCAGCTC 
TTCCCAGATG GGCATTTTCC TGAGAACTTT TCTGTGCTGC TCACACTGAG GGCCCAGCCA 
GCCAATCAGT CTGTCCTTCT GTCTATTTAT GATGAGAAGG GTGTCCGGCA GCTGGGGCTG 
GCACTGGGGC CAGCTCTGGG CCTCCTTGGT GACTCCTTCA GGCCCCTCCC CAAGCAAGTC 
AACATTATGG ATGGCAGGTG GCACCGTGTG GCAGTCAGCA TCAGTGGTAA CAAGGTGACC 
CTGGTGGTTG ACTGTGAACC GCAGCCCCCA ACATTTGGTC AGGGGCCTCG GTTTATAAGT 
ACAGCTGGAC TCACTGTGAT GGGAACCCAG GACACCAGGG AAGAGTCTTT TGAGGGAGAC 
ATCCAGGAGC TGCTGTTAAT TCCAGACCCT CAGGCTGCCT TCCAGGCCTG TGAGAGCTAC 
CTCCCTGGTT GTGAAACCCT CGATTCCACA ACCACAGGGG CCCCCAAAGA CGATGAACCA 
GAAACCCCTG CCCCTCGTCG TCGAAAGGGC AAAGGGAAGA AAAAAGGGCG GGGTCGAAAG 
GGCAAGGGAA GAAAGAAAAA CAAGGAGACC TCAGAGCTGA GTCCGACCCC TGGTGCCCCT 
GAGAACCAGA CCTCCCTCCA CATCCCTGAG ACAGAGAAGA CAGTTCCCCA CCTGCCTCTG 
ACTCCCACAC CTCTGGCCAT CACCACCACT GTCACGATTG GACAAAATGC CACAGTCTCG 
CAGGGGTTGG ACTCCGGTAC TGAAACCGAG CAGACGACTC CAGAGGTGGA CTCTACTGAG 
GAGGGTGAAG GAGGTGGCCC CACCATGGGC CCCAAGTTCC GGGCAGCAGA GCAGTCCTTA 
CAGACTGAGT TCCAGATCTT TCCTGGTGCT GGAGAAAAGG GAGCGAAAGG AGAGCCTGCG 
ACAGTAGAGC AGGGACAGCA GTTTGAGGGG CCTGCAGGAG CTCCAGGACC CCGGGGAATA 
TCTGGTCCTT CAGGCCCTCC TGGGCCTCCG GGCTTCCCTG GGGACCGTGG TCTACCGGGT 
CCTGCCGGCC TCCCAGGAAT CCCAGGCATC GATGGAGCCC GGGGCCTGCC GGGCACAGTG 
ATTATGATGC CGTTCCATTT TGCAAGCAGC TCGATGAAGG GACCCCCAGT GTCCTTCCAG 
CAGGCCCAGG CCCAGGCAGT ATTGCAACAG GCTCAGCTGT CCATGAAAGG GCCCCCTGGT 
CCAGTAGGGC TCACTGGGCG CCCAGGCCCT GTGGGCCTCC CTGGATATCC AGGTCTGAAA 
GGTGAACTGG GAGAAGTGGG GCCACAGGGC CCCCGAGGAT TACAGGGCCC TCCTGGGCCT 
CCTGGACGGG AAGGCAAGAC AGGCCGAGCT GGAGCAGATG GGGCTCGGGG GCTCCCGGGA 
GACACAGGAC CTAAGGGTGA CAGGGGCTTT GATGGCCTGC CCGGGCTGCC TGGTGAGAAG 
GGCCAAAGGG GTGACTTTGG ACGAGTAGGG CAACCTGGTC CCCCAGGAGA GGATGGTGTA 
AAGGGCCTGC AGGGACCTCC AGGGCCCACT GGCCAGGCTG GAGAGCCGGG TCCCCGAGGT 
CTGATTGGCC CCAGAGGTCT CCCAGGTCCC CTAGGACGCC CGGGTGTGAC AGGGAGTGAT 
GGCGCACCAG GGGCCAAAGG CAACGTGGGT CCTCCTGGAG AACCAGGACC CCCAGGACAG 
CAAGGAAACC ACGGCTCCCA GGGAATTCCA GGCCCCCAGG GGCCCATTGG CACTCCCGGG 
GAAAAGGGTC CCCCTGGAAA CCCCGGAATT CCAGGTGTCC CAGGATCTGA GGGCCCCCCG 
GGCCACCCAG GCCACGAGGG TCCCACAGGA GAAAAAGGGG CTCAGGGCCC ACCAGGATCA 
GCAGGCCCTC GGGGCTATCC TGGACTTCGT GGTGTGAAGG GTACCTCTGG TAACCGGGGT 
CTCCAAGGCG AGAAAGGAGA AAGGGGAGAG GATGGCTTTC CTGGCTTCAA GGGTGATGAG 
GGACCAAAAG GCGACCGGGG AAACCCCGGA CCCCCAGGTC CCAGAGGAGA GGATGGTCCA 
GAAGGACAAA AGGGGCCTGG GGGACTGCCT GGTGATGAGG GTCCTCCAGG AGCAGCAGGG 
GAGAAGGGCA AGCTTGGGGT GCCAGGTCTC CCAGGTTATC CAGGACGCCC AGGACCTAAG 
GGATCTATTG GATTTCCTGG ACCCTTGGGA CCACTGGGGG AGAAAGGCAA GCGGGGCAAA 
GCAGGACAGC CAGGAGAGGA AGGAGAACGC GGCACACCGG GCACCCGAGG AGACAGGGGA 
CAGCCGGGGG CCACAGGCCA GCCTGGCCCC AAGGGTGACG TGGGCCAGAA TGGGTCTCCT 
GGGCCCCCTG GAGAAAAGGG TCTACCCGGT CTTCAAGGCC CACCAGGATT CCCCGGACCA 
AAAGGCCCCC CGGGTCCTCA GGGGAAAGAT GGGATATCTG GGCACCCTGG ACAAAGAGGA 
GAATTGGGCT TCCAAGGTCT GACAGGCCCC CCTGGACCAG CTGGCGTCCT TGGTCCTCAG 
GGAAAGGTAG GGGACGTGGG GCCTCTAGGC GAGAGAGGCC CCCCAGGGCC TCCTGGACCT 
CCTGGTGAAC AAGGTCTGCC AGGCATAGAA GGCAGAGAAG GGGCCAAGGG TGAGCTAGGA 
CCCCTGGGGT CCGTCGGGAA GGAGGGGCCA CCTGGGCCCA GGGGCTTCCC TGGCCCCCAA 
GGAGCCCCCG GAGACCCAGG ACCCATTGGT TTGAAGGGTG ACAAAGGTCC CCCAGGCCCT 
GTTGGGGCAA ATGGCTCCCC GGGAGAGCGT GGTCCTGTAG GCCCCTCTGG TGGCATTGGG 
CTTCCTGGCC AGAGTGGAGG GCAAGGCCCT ATTGGTCCTG CTGGCGAGAA GGGGTCCCCG 
GGAGAACGGG GTACTCCTGG TCCTACTGGC AAAGATGGTA TTCCAGGACC CCCGGGGCTT 
CAGGGCCCCT CTGGAGCTGC GGGGCCTTCT GGGGAAGAAG GAGACAAGGG GGAAGTAGGG 
ATGCCTGGTC ACAAAGGAAG CAAAGGGGAT AAAGGAGATG CAGGCCCACC TGGACCAACA 
GGAATAAGAG GTCCAGCAGG CCATTCAGGC CTCCCGGGTG CTGATGGCGC TCAGGGTCGC 
CGGGGACCCC CTGGCCTCTT CGGGCAGAAG GGGGATGACG GAGTTCGAGG CTTTGTAGGT 
GTAATTGGTC CTCCAGGTCT GCAGGGGCTG CCGGGTCCTC CGGGGGAGAA GGGCGAGGTT 
GGAGACGTAG GATCCATGGG TCCACATGGA GCTCCAGGCC CTCGGGGTCC CCCTGGGCCC 
AGTGGATCAG AGGGCCCCCC AGGTCTGCCT GGAGGAGTAG GACAGCCTGG TGCTGTGGGC 
GAGAAGGGTG AGCCAGGGGA TGCTGGAGAC GCCGGACCCC CAGGAATTCC CGGCATCCCT 
GGGCCCAAAG GTGAAATTGG TGAAAAGGGG GATTCGGGTC CATCAGGGGC TGCTGGTCCC 
CCAGGCAAGA AAGGACCCCC AGGAGAGGAC GGCTCTAAGG GGAACATGGG TCCCACAGGA 
CTCCCTGGAG ATCTAGGGCC CCCAGGAGAC CCTGGAGTTC CGGGTATTGA TGGCATCCCA 
GGGGAGAAGG GAAATGCTGG TGATATTGGG GGACCGGGGC CACCTGGAGC TTCCGGGGAA 
CCTGGTGCCC GTGGCCTCCC TGGCAAGAGG GGTTCCCCTG GCCGCATGGG TCCAGAAGGA 
AGAGAGGGCG AGAAAGGCGC CAAGGGAGAT GCTGGTCCTG ATGGACCCCC AGGCAGGACA 
GGCCCCATTG GGGCTCGAGG GCCCCCTGGA CGAATTGGGC CTGATGGTCT TCCAGGGATC 
CCTGGTCCTG TGGGTGAACC AGGTCTCCTG GGACCTCCTG GGCTAATCGG CCCTCCAGGG 
CCCCTGGGCC CACCTGGCCT CCCTGGCCTG AAGGGAGATG CTGGCCCCAA GGGGGAGAAG 
GGCCACATTG GGCTAATAGG CCTCATTGGT CCCCCAGGGG AGGCCGGTGA GAAAGGCGAT 
CAGGGGTTGC CAGGTGTGCA GGGCCCCCCA GGCCTTCAGG GAGACCCTGG TCTCCCTGGT 
CCTGTTGGCT CGTTAGGTCA CCCTGGGCCC CCAGGTGTGG TGGGCCCTCT GGGACAGAAG 
GGCTCCAAAG GGTCCCCGGG ATCTCTTGGT CCTCGTGGAG ACCCTGGACC AGCGGGTCCT 
CCTGGTCCCC CGGGTTCTCC GGCTGAGGTG CATGGCCTGC GCAGGCGCCG ATCTGTGACG 
GACACCCTGG AAGGTGGCCT GGAGGAGGTG ATGGCCTCAC TGAATTCACT GAGCTTGGAG 
CTGCAGCAGT TGCAGAGACC TCTGGGCACA GCCGAGAGCC CAGGCCTCAT GTGCCGAGAG 
CTTCACCGCG ACCACCCACA CCTGCCCGAT GGAGAGTACT GGATTGACCC CAATCAGGGC 
TGTGCACGTG ACGCCTTCAA GGTTTTCTGC AACTTCACGG CAGGAGGTGA GACCTGTCTC 
TATCCAGACA AGAAGTTTGA GACGGTGAAA CTGGCCTCGT GGTCCCGAGA GAAGCCTGGA 
GGCTGGTACA GCACCTTCCG CCGAGGGAAG AAGTTCTCCT ATGTGGATGC TGATGGCTCC 
CCGGTGAATG TGGTCCAGTT GACCTTCCTG AAGTTGTTGA GTGCTGCAGC CCATCAGAGG 
TTCACTTACA TCTGCCAGAA CTCGGTGGCA TGGCTGGATG AAGCTGCGGG TGACCACAGG 
CACTCCATCC GCTTCCAAGG GACCAACTGG GAAGAGTTGT CCTTCAACCA GACAACAGCA 
GCTACCATCA AGGTCTCCCA TGATGGCTGT CGGGTCCGGA AGGGACAGGC GAAGACCCTC 
TTTGAATTCA GCTCTTCTGT GGGTTTCCTG CCTCTGTGGG ATGTGGCTGC CTCTGACTTT 
GGTCAGACGA ACCAAAAGTT TGGGTTTGAA CTCGGCTCCA TCTGCTTTAG CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Scientific reports740495(2017 Jan)
Naba A,Clauser KR,Mani DR,Carr SA,Hynes RO

?3 Chains of type V collagen regulate breast tumour growth via glypican-1.
Nature communications814351(2017 Jan)
Huang G,Ge G,Izzi V,Greenspan DS

The matrisome: in silico definition and in vivo characterization by proteomics of normal and tumor extracellular matrices.
Molecular & cellular proteomics : MCP11(4)M111.014647(2012 Apr)
Naba A,Clauser KR,Hoersch S,Liu H,Carr SA,Hynes RO

Transient expression of mouse pro-?3(V) collagen gene (Col5a3) in wound healing.
Connective tissue research53(4)313-7(2012)
Sumiyoshi H,Kitamura H,Matsuo N,Tatsukawa S,Ishikawa K,Okamoto O,Fujikura Y,Fujiwara S,Yoshioka H

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Col5a3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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