COL5A3 cDNA ORF clone, Bos taurus(cattle)

The following COL5A3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL5A3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa235868 XM_010806810.3
Latest version!
Bos taurus collagen type V alpha 3 chain (COL5A3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa235869 XM_010806811.3
Latest version!
Bos taurus collagen type V alpha 3 chain (COL5A3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa235870 XM_010806813.3
Latest version!
Bos taurus collagen type V alpha 3 chain (COL5A3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa235871 XM_005208735.4
Latest version!
Bos taurus collagen type V alpha 3 chain (COL5A3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa235872 XM_015472002.2
Latest version!
Bos taurus collagen type V alpha 3 chain (COL5A3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa235873 XM_024993999.1
Latest version!
Bos taurus collagen type V alpha 3 chain (COL5A3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa235874 XM_024994000.1
Latest version!
Bos taurus collagen type V alpha 3 chain (COL5A3), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa216324 NM_001205624.2
Latest version!
Bos taurus collagen type V alpha 3 chain (COL5A3), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa40506 NM_001205624.3
Latest version!
Bos taurus collagen type V alpha 3 chain (COL5A3), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa21009 XM_005208735.3
Latest version!
Bos taurus collagen type V alpha 3 (COL5A3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa46992 XM_010806810.2
Latest version!
Bos taurus collagen type V alpha 3 (COL5A3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa46993 XM_010806811.2
Latest version!
Bos taurus collagen type V alpha 3 (COL5A3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa46994 XM_010806813.2
Latest version!
Bos taurus collagen type V alpha 3 (COL5A3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa46996 XM_010806815.3
Latest version!
Bos taurus collagen type V alpha 3 chain (COL5A3), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa91062 XM_015472002.1
Latest version!
Bos taurus collagen type V alpha 3 (COL5A3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
View Old Accession Versions >>
OBa46996 XM_010806815.2 Bos taurus collagen type V alpha 3 (COL5A3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa40506 NM_001205624.1 Bos taurus collagen, type V, alpha 3 (COL5A3), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa235868
    Clone ID Related Accession (Same CDS sequence) XM_010806810.3
    Accession Version XM_010806810.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-3(V) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806810.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCCGGAC GCAGGCCGTA GATCCTGTGG ATGTGCTGAA GGCACTGGGC
    ATGCGGGGGG GCCAGGCTGG GATCCCTGAG GGACCCGGCC TCTGCCCCCA GAGGGTCCCG
    GAGGGCGACC GAGCATTCCG GGTGGGCAAG ACTGGCACAC TTGGTGTCCC CACGCAGGAG
    CTCTTCCAAG ATGGGCACTT TCCTGAGAAC TTCTCCATCC TGATCACCCT GCGGGGCCAG
    CCGGCCAACC GTTCTGTCCT TCTGTCCATC TATGATGAGG GCGGCGTCCG GCAGCTCGGC
    TTGGCCTTGG GGCCAGCTCT GGACCTCCTA GGTGACTCCT TCAGCCCCCT CCCCCAGCAG
    GTCAACCTCA CGGATGGCAG GTGGCACCGT GTGGCAGTCA GTGTGGACGG TGGGATGGTG
    ACCCTGGTGG CTGATTGTGA ACCTCAGCTC CCCATGTTGG CTCAGGGACC TCGATTCATC
    AGTACAGCGG GACTCACTGT GCTGGGGACC CAGGACCTTG GCGAGGAGAC TTTTGAGGGA
    GATGTTCAGG AGCTGGTGAT AAGCCCAGAT CCCCAGGCTG CCTTCCAGGC ATGTGAGCGG
    TACCTTCCTG GCTGTGATGA CCTGGATCCA ACAGTCACCA CGGTCCCTCA GGGGGAGCCA
    GAAACCCCTC CCCCTAGGCG GAAGGGGAAA GGAAAAGGGA AGAAAAAAGG GCGAGGTCGT
    AAAGGGAAGG GCAGGAAGAA GAAGAACAAG GAAACTGTGG CTGTGAGTCC TCCTCCTGGC
    TCCCTGGACA ACCAGACCTC CACTGACATC CCCAAGACAG AGACACCAGA ACCAACTCTG
    TCTCCGACAC CCACGCCTTT GGTCATCACC ACAACTGTGA CCTTTGGTAG CAATGTCACC
    ATCCTAGAGG AGGGCCTGGA CCCTGACAAT GGAACTGAAC TGGAGACCCT GGAGACTGAG
    TTAGACAGAG AAGATGAAGA AGAAGGTGAT CCCACCATGG GCCCCCACTT CCGGGCAGCA
    GAACAGTCAT GGAATACTGA GTTCCAGATC TTTCCTGGTG CTGGGGAGAA AGGAGAAAAA
    GGAGAACCAG CTGTGGTCGA ACAGGGACAG CAGTTTGAAG GACCCCCAGG AGCCCCGGGA
    CCCCAAGGGG TGGTTGGCCC CTCAGGCCCT CCTGGCCCCC CAGGATTCCC TGGAGACCGT
    GGTCTACCGG GCCCTGCTGG CCTCCCAGGC ATCCCTGGCA TGGATGGGAT CCGGGGTCTC
    CCAGGCACTG TGATCATGAT GCCGTTCCAG TTTGCAAGAG GCTCTCACAA AGGACCCCCA
    GTATCCTTCC AGCAGGCCCA GGCTCAGGCA GTCCTGCAAC AAGCTCAGCT GTCTATGAAA
    GGCCCTCCTG GCCCGGTGGG GCTCACCGGA CGCCCAGGTC CCGTGGGTCT TCCTGGGTAT
    CCAGGTCTGA AAGGAGAGAT GGGAGAAATG GGGCCGCAGG GTCCCCGAGG GCTTCAGGGA
    CCTCATGGGC CCCCTGGCCG AGAGGGAAAG ATGGGCCGCG CTGGAGCAGA TGGGGCTCGG
    GGCCTCCCCG GCGCTACGGG ACCTAAGGGT GATCGGGGCT TTGATGGCCT GCCAGGGCTG
    CCTGGTGAGA AGGGCCAAAG GGGTGACTTT GGTTATGTGG GGAAACCTGG TCCCCCAGGA
    GAGGATGGAC GGAAGGGAGC AGAGGGACCT CCGGGGCCCA CTGGCCAGGC AGGGGAGCCG
    GGCCCCCGAG GACTGATTGG CCCTAGAGGC TCCCCCGGAC CCCTGGGACG CCCGGGTGTG
    ATTGGAAGTG ATGGTGCTCC AGGTGCCAAA GGAAATGTGG GTCCTCCAGG AGAACCAGGC
    CCCCCAGGAC AGCAGGGAAA TCCTGGGTCC CAGGGAATTC CCGGCCCCCA GGGACCCATC
    GGCACTCCTG GGGAGAAGGG TCCTCCTGGA AACCCAGGAA TTCCAGGCCT CCCAGGATCT
    GAAGGCCCTC CGGGTCACCC AGGTCATGAG GGCCCAACAG GAGAGAAAGG GGCCCAGGGT
    CCAGCAGGGT CAGCAGGCCC TCCGGGCTAT CCAGGACCTC GGGGTGTGAA GGGTACCTCT
    GGCAACCGGG GCCTCCAGGG GGAGAAAGGA GAGAAGGGAG AGGATGGTTT CCCAGGCTTC
    AAGGGTGATG CGGGGCTTAA AGGAGATCGG GGGCAACCCG GACCTCCAGG TCCTCGGGGA
    GAGGATGGTC CGGAGGGGCT AAAGGGGCAG GAGGGGCTGC TTGGCGAGGA GGGTCCCCCA
    GGCTCAGCTG GGGAGAAGGG CAAGCTTGGC GTGCCGGGTC TCCCAGGTTA TCCAGGACGT
    CCAGGCCCTA AGGGATCTAT TGGCTTTCCG GGACCTCTGG GACCATTAGG AGAAAAAGGG
    AAGCGGGGGA AGGCTGGGCA GCCAGGCCTG GAAGGAGAGC GGGGACCACC AGGTGCCCGT
    GGAGAGAGAG GGCAACCAGG TGCCACAGGG CAGCCGGGCC CCAAGGGTGA TGTGGGCCAG
    GACGGAGCCC CTGGGTTCCC TGGAGAAAAG GGCCTCCCAG GTCTGCAAGG TCCTCCTGGG
    TTCTCTGGAC CAAAGGGTCC CCCTGGTCCC CAGGGAAAAG ATGGGGTACC TGGACACCCT
    GGACAGAGAG GAGAATTGGG TTTCCAAGGT CAGACAGGCC CACCTGGACC AGCTGGTGTC
    GTGGGTCCTC AGGGAAAGAC AGGAGAAGCA GGGCCTCTGG GTGAGAGGGG GCCCCCAGGG
    CCCCCTGGAC CTCCTGGTGA ACAAGGTCTT CCGGGCCTGG AAGGCAGAGA GGGGGCCAAG
    GGGGATGTGG GACCACTGGG ACCCCTTGGG AAGGAAGGGC CACCTGGACC CAGAGGTTTC
    CCTGGTCCTC ATGGAGCCCC CGGGGACCCA GGACCGACTG GTTTAAAGGG TGACAAGGGC
    CCCCCAGGTC CTGTTGGTGC CAACGGATCC CCTGGGGAGC GTGGTCCTGT AGGCCCAGCA
    GGGAGCATTG GGCTTCCTGG TCAAAGCGGA GGCCAAGGCC CCGTGGGCCC CACAGGCGAG
    AAGGGTTCCC CGGGTGAACG TGGCGCCCCT GGCCCAACTG GTAAAGATGG GATCCCCGGG
    CCCCTGGGGC TTCCTGGGCC CCCTGGAGCT GCGGGGCCTT CTGGCGAGGA AGGGGACAAG
    GGAGAAGTGG GTGCCCCCGG TCACAAAGGC AGCAAAGGTG ACAAGGGAGA TGCGGGCCCG
    CCTGGACCAA CAGGGATACG GGGTCTGGTG GGACACCCCG GCTCCCCGGG AGCAGACGGG
    GCTCAGGGAC GCCGGGGACC CCCAGGCCTC TTTGGGCAGA AAGGAGATGA TGGAGTGAGA
    GGCTTCCCTG GCATGATTGG TCCTCCTGGC CTACAGGGAA TGCCAGGCCC TGCTGGAGAA
    AAGGGAGAAG TTGGAGATGT AGGGTCCATG GGTCCCCACG GAGCTCCAGG CCCTCGGGGC
    CCCCAGGGCC CCAGCGGATC AGAGGGTCCT CCAGGGTTGC CTGGGGGAGT TGGTCAGCCT
    GGCGCTGTGG GGGAGAAGGG TGAGCCAGGA GAGCCTGGAG ACCCAGGACC CCCCGGGACC
    CCAGGCACCC CGGGCCCCAG GGGAGAAGTT GGTGAAAAGG GGGATACAGG CCCATCTGGG
    GCTGCTGGAC CCCCAGGCAA GAAAGGACCC CCTGGAGAGG ATGGAGCAAA GGGGAATGTG
    GGCCCCACAG GCCTCCCAGG AGACCTAGGC CCCCCCGGAG ACCCTGGAGT TTCGGGTCAA
    GATGGCCCCC CAGGGGAGAA GGGAAACCCT GGAGATGTGG GGGGACCGGG TCCACCTGGC
    GCTTCTGGGG AGCCTGGCGC CCCTGGGCCT CCTGGTAAAA GGGGTCCTTC TGGTCGCATG
    GGTCAAGAAG GTAGAGAAGG AGAGAAAGGT GCCAAGGGGG AACCAGGTCC TGATGGACCC
    CCAGGGAGGA CAGGCCCAGT AGGGGCTCGA GGGCCCCCTG GACGTGTTGG ACCTGAGGGT
    CTTCGTGGGA TCCCTGGACC TGTGGGCGAA CCAGGCCTCC TGGGACCTCC TGGACTGTTA
    GGCCCTCCTG GTCCCCTGGG GCCCTCTGGC CTACCAGGGC TGAAGGGAGA TGCTGGCCCC
    AAGGGAGAAA AGGGCCACAT TGGATTAATT GGTCTTGTCG GCCCCCCTGG GGAGGTTGGT
    GAGAAAGGGG ATCAGGGGTT GCCAGGAGTG CAAGGCTCCC CTGGCCTCCA GGGAGAACCG
    GGTTCTCCTG GCCCCATTGG CTCTCTGGGC CACCCTGGGC CCCAAGGCGT GGTGGGTCCT
    CCAGGACAGA AAGGCTCAAA GGGGTCCCCG GGATCCCAAG GTCCTCGTGG AGACGCTGGC
    CCCCCAGGTC CTCCTGGTCC CCCGGGTCCC TCTGCGGAGC TGCACGGGCT GCGCCGGCGC
    CGACGTTCCC TGCTAGGCTT GGGAACCCCG GAGGGCGGCC TGGAGGAGGT GCTGGCCTCG
    ATGGCGTCGC TGAGCTTCGA GTTAGAGCAG CTGCGGCGCC CTCCCGGCAC GGCTGAGCGC
    CCGGGCCTCG TGTGCAAAGA GTTGCACCGC AGCCATCCGC ATCTGCCAGA TGGGGAATAC
    TGGATTGATC CCAATCAAGG CTGCGCGCGG GATGCCCTCA GGGTTTTCTG TAACTTCACG
    GCAGGAGGAG AAACCTGCCT CTACCCAGAC AAGAAGTTTG AGACCGTGAA ACTGGCCTCT
    TGGTCCAAAG AAAAGCCAGG TGGCTGGTAT AGCACATTCC GTCGAGGGAA AAAGTTCTCC
    TACGTGGATG CCGATGGGGC CGCAGTGAGC GTGGTACAGC TGACCTTCCT GAAGTTGCTG
    AGTGCCACGG CCCGCCAGAG CTTCACATTC ACCTGCCAGA ACTCGGCCGC CTGGCTGGAC
    GAAGCTGCAG GTGACCACAG CCGCTCTGCC CGTTTCCTCG GGGCCAATGG GGAGGAGGTA
    TCCTTCAACC AGATGGCAGC CGCCACCATC ACAGTCCCCT ACGATGGCTG CAGGCTCCGG
    AAGGGACAGT CGAAGACCCT CTTTGAGTTC AGCTCCTCCC GAGTGGGATT TCTGCCCCTG
    TTGGACGTGG CAGCTGCTGA CTTTGGCCAG ACGAACCAGA AGTTTGGGTG GGTTCGCAGT
    GAAGGGACTG ATGTCAGACT ACAGAGGGAA GTGGGACTTC CCTGGACTGA GCTGCGTACT
    CTTTCTGCTG CCTCAGCCCA GCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805112.2
    CDS157..5463
    Translation

    Target ORF information:

    RefSeq Version XM_010806810.3
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type V alpha 3 chain (COL5A3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806810.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCCGGAC GCAGGCCGTA GATCCTGTGG ATGTGCTGAA GGCACTGGGC
    ATGCGGGGGG GCCAGGCTGG GATCCCTGAG GGACCCGGCC TCTGCCCCCA GAGGGTCCCG
    GAGGGCGACC GAGCATTCCG GGTGGGCAAG ACTGGCACAC TTGGTGTCCC CACGCAGGAG
    CTCTTCCAAG ATGGGCACTT TCCTGAGAAC TTCTCCATCC TGATCACCCT GCGGGGCCAG
    CCGGCCAACC GTTCTGTCCT TCTGTCCATC TATGATGAGG GCGGCGTCCG GCAGCTCGGC
    TTGGCCTTGG GGCCAGCTCT GGACCTCCTA GGTGACTCCT TCAGCCCCCT CCCCCAGCAG
    GTCAACCTCA CGGATGGCAG GTGGCACCGT GTGGCAGTCA GTGTGGACGG TGGGATGGTG
    ACCCTGGTGG CTGATTGTGA ACCTCAGCTC CCCATGTTGG CTCAGGGACC TCGATTCATC
    AGTACAGCGG GACTCACTGT GCTGGGGACC CAGGACCTTG GCGAGGAGAC TTTTGAGGGA
    GATGTTCAGG AGCTGGTGAT AAGCCCAGAT CCCCAGGCTG CCTTCCAGGC ATGTGAGCGG
    TACCTTCCTG GCTGTGATGA CCTGGATCCA ACAGTCACCA CGGTCCCTCA GGGGGAGCCA
    GAAACCCCTC CCCCTAGGCG GAAGGGGAAA GGAAAAGGGA AGAAAAAAGG GCGAGGTCGT
    AAAGGGAAGG GCAGGAAGAA GAAGAACAAG GAAACTGTGG CTGTGAGTCC TCCTCCTGGC
    TCCCTGGACA ACCAGACCTC CACTGACATC CCCAAGACAG AGACACCAGA ACCAACTCTG
    TCTCCGACAC CCACGCCTTT GGTCATCACC ACAACTGTGA CCTTTGGTAG CAATGTCACC
    ATCCTAGAGG AGGGCCTGGA CCCTGACAAT GGAACTGAAC TGGAGACCCT GGAGACTGAG
    TTAGACAGAG AAGATGAAGA AGAAGGTGAT CCCACCATGG GCCCCCACTT CCGGGCAGCA
    GAACAGTCAT GGAATACTGA GTTCCAGATC TTTCCTGGTG CTGGGGAGAA AGGAGAAAAA
    GGAGAACCAG CTGTGGTCGA ACAGGGACAG CAGTTTGAAG GACCCCCAGG AGCCCCGGGA
    CCCCAAGGGG TGGTTGGCCC CTCAGGCCCT CCTGGCCCCC CAGGATTCCC TGGAGACCGT
    GGTCTACCGG GCCCTGCTGG CCTCCCAGGC ATCCCTGGCA TGGATGGGAT CCGGGGTCTC
    CCAGGCACTG TGATCATGAT GCCGTTCCAG TTTGCAAGAG GCTCTCACAA AGGACCCCCA
    GTATCCTTCC AGCAGGCCCA GGCTCAGGCA GTCCTGCAAC AAGCTCAGCT GTCTATGAAA
    GGCCCTCCTG GCCCGGTGGG GCTCACCGGA CGCCCAGGTC CCGTGGGTCT TCCTGGGTAT
    CCAGGTCTGA AAGGAGAGAT GGGAGAAATG GGGCCGCAGG GTCCCCGAGG GCTTCAGGGA
    CCTCATGGGC CCCCTGGCCG AGAGGGAAAG ATGGGCCGCG CTGGAGCAGA TGGGGCTCGG
    GGCCTCCCCG GCGCTACGGG ACCTAAGGGT GATCGGGGCT TTGATGGCCT GCCAGGGCTG
    CCTGGTGAGA AGGGCCAAAG GGGTGACTTT GGTTATGTGG GGAAACCTGG TCCCCCAGGA
    GAGGATGGAC GGAAGGGAGC AGAGGGACCT CCGGGGCCCA CTGGCCAGGC AGGGGAGCCG
    GGCCCCCGAG GACTGATTGG CCCTAGAGGC TCCCCCGGAC CCCTGGGACG CCCGGGTGTG
    ATTGGAAGTG ATGGTGCTCC AGGTGCCAAA GGAAATGTGG GTCCTCCAGG AGAACCAGGC
    CCCCCAGGAC AGCAGGGAAA TCCTGGGTCC CAGGGAATTC CCGGCCCCCA GGGACCCATC
    GGCACTCCTG GGGAGAAGGG TCCTCCTGGA AACCCAGGAA TTCCAGGCCT CCCAGGATCT
    GAAGGCCCTC CGGGTCACCC AGGTCATGAG GGCCCAACAG GAGAGAAAGG GGCCCAGGGT
    CCAGCAGGGT CAGCAGGCCC TCCGGGCTAT CCAGGACCTC GGGGTGTGAA GGGTACCTCT
    GGCAACCGGG GCCTCCAGGG GGAGAAAGGA GAGAAGGGAG AGGATGGTTT CCCAGGCTTC
    AAGGGTGATG CGGGGCTTAA AGGAGATCGG GGGCAACCCG GACCTCCAGG TCCTCGGGGA
    GAGGATGGTC CGGAGGGGCT AAAGGGGCAG GAGGGGCTGC TTGGCGAGGA GGGTCCCCCA
    GGCTCAGCTG GGGAGAAGGG CAAGCTTGGC GTGCCGGGTC TCCCAGGTTA TCCAGGACGT
    CCAGGCCCTA AGGGATCTAT TGGCTTTCCG GGACCTCTGG GACCATTAGG AGAAAAAGGG
    AAGCGGGGGA AGGCTGGGCA GCCAGGCCTG GAAGGAGAGC GGGGACCACC AGGTGCCCGT
    GGAGAGAGAG GGCAACCAGG TGCCACAGGG CAGCCGGGCC CCAAGGGTGA TGTGGGCCAG
    GACGGAGCCC CTGGGTTCCC TGGAGAAAAG GGCCTCCCAG GTCTGCAAGG TCCTCCTGGG
    TTCTCTGGAC CAAAGGGTCC CCCTGGTCCC CAGGGAAAAG ATGGGGTACC TGGACACCCT
    GGACAGAGAG GAGAATTGGG TTTCCAAGGT CAGACAGGCC CACCTGGACC AGCTGGTGTC
    GTGGGTCCTC AGGGAAAGAC AGGAGAAGCA GGGCCTCTGG GTGAGAGGGG GCCCCCAGGG
    CCCCCTGGAC CTCCTGGTGA ACAAGGTCTT CCGGGCCTGG AAGGCAGAGA GGGGGCCAAG
    GGGGATGTGG GACCACTGGG ACCCCTTGGG AAGGAAGGGC CACCTGGACC CAGAGGTTTC
    CCTGGTCCTC ATGGAGCCCC CGGGGACCCA GGACCGACTG GTTTAAAGGG TGACAAGGGC
    CCCCCAGGTC CTGTTGGTGC CAACGGATCC CCTGGGGAGC GTGGTCCTGT AGGCCCAGCA
    GGGAGCATTG GGCTTCCTGG TCAAAGCGGA GGCCAAGGCC CCGTGGGCCC CACAGGCGAG
    AAGGGTTCCC CGGGTGAACG TGGCGCCCCT GGCCCAACTG GTAAAGATGG GATCCCCGGG
    CCCCTGGGGC TTCCTGGGCC CCCTGGAGCT GCGGGGCCTT CTGGCGAGGA AGGGGACAAG
    GGAGAAGTGG GTGCCCCCGG TCACAAAGGC AGCAAAGGTG ACAAGGGAGA TGCGGGCCCG
    CCTGGACCAA CAGGGATACG GGGTCTGGTG GGACACCCCG GCTCCCCGGG AGCAGACGGG
    GCTCAGGGAC GCCGGGGACC CCCAGGCCTC TTTGGGCAGA AAGGAGATGA TGGAGTGAGA
    GGCTTCCCTG GCATGATTGG TCCTCCTGGC CTACAGGGAA TGCCAGGCCC TGCTGGAGAA
    AAGGGAGAAG TTGGAGATGT AGGGTCCATG GGTCCCCACG GAGCTCCAGG CCCTCGGGGC
    CCCCAGGGCC CCAGCGGATC AGAGGGTCCT CCAGGGTTGC CTGGGGGAGT TGGTCAGCCT
    GGCGCTGTGG GGGAGAAGGG TGAGCCAGGA GAGCCTGGAG ACCCAGGACC CCCCGGGACC
    CCAGGCACCC CGGGCCCCAG GGGAGAAGTT GGTGAAAAGG GGGATACAGG CCCATCTGGG
    GCTGCTGGAC CCCCAGGCAA GAAAGGACCC CCTGGAGAGG ATGGAGCAAA GGGGAATGTG
    GGCCCCACAG GCCTCCCAGG AGACCTAGGC CCCCCCGGAG ACCCTGGAGT TTCGGGTCAA
    GATGGCCCCC CAGGGGAGAA GGGAAACCCT GGAGATGTGG GGGGACCGGG TCCACCTGGC
    GCTTCTGGGG AGCCTGGCGC CCCTGGGCCT CCTGGTAAAA GGGGTCCTTC TGGTCGCATG
    GGTCAAGAAG GTAGAGAAGG AGAGAAAGGT GCCAAGGGGG AACCAGGTCC TGATGGACCC
    CCAGGGAGGA CAGGCCCAGT AGGGGCTCGA GGGCCCCCTG GACGTGTTGG ACCTGAGGGT
    CTTCGTGGGA TCCCTGGACC TGTGGGCGAA CCAGGCCTCC TGGGACCTCC TGGACTGTTA
    GGCCCTCCTG GTCCCCTGGG GCCCTCTGGC CTACCAGGGC TGAAGGGAGA TGCTGGCCCC
    AAGGGAGAAA AGGGCCACAT TGGATTAATT GGTCTTGTCG GCCCCCCTGG GGAGGTTGGT
    GAGAAAGGGG ATCAGGGGTT GCCAGGAGTG CAAGGCTCCC CTGGCCTCCA GGGAGAACCG
    GGTTCTCCTG GCCCCATTGG CTCTCTGGGC CACCCTGGGC CCCAAGGCGT GGTGGGTCCT
    CCAGGACAGA AAGGCTCAAA GGGGTCCCCG GGATCCCAAG GTCCTCGTGG AGACGCTGGC
    CCCCCAGGTC CTCCTGGTCC CCCGGGTCCC TCTGCGGAGC TGCACGGGCT GCGCCGGCGC
    CGACGTTCCC TGCTAGGCTT GGGAACCCCG GAGGGCGGCC TGGAGGAGGT GCTGGCCTCG
    ATGGCGTCGC TGAGCTTCGA GTTAGAGCAG CTGCGGCGCC CTCCCGGCAC GGCTGAGCGC
    CCGGGCCTCG TGTGCAAAGA GTTGCACCGC AGCCATCCGC ATCTGCCAGA TGGGGAATAC
    TGGATTGATC CCAATCAAGG CTGCGCGCGG GATGCCCTCA GGGTTTTCTG TAACTTCACG
    GCAGGAGGAG AAACCTGCCT CTACCCAGAC AAGAAGTTTG AGACCGTGAA ACTGGCCTCT
    TGGTCCAAAG AAAAGCCAGG TGGCTGGTAT AGCACATTCC GTCGAGGGAA AAAGTTCTCC
    TACGTGGATG CCGATGGGGC CGCAGTGAGC GTGGTACAGC TGACCTTCCT GAAGTTGCTG
    AGTGCCACGG CCCGCCAGAG CTTCACATTC ACCTGCCAGA ACTCGGCCGC CTGGCTGGAC
    GAAGCTGCAG GTGACCACAG CCGCTCTGCC CGTTTCCTCG GGGCCAATGG GGAGGAGGTA
    TCCTTCAACC AGATGGCAGC CGCCACCATC ACAGTCCCCT ACGATGGCTG CAGGCTCCGG
    AAGGGACAGT CGAAGACCCT CTTTGAGTTC AGCTCCTCCC GAGTGGGATT TCTGCCCCTG
    TTGGACGTGG CAGCTGCTGA CTTTGGCCAG ACGAACCAGA AGTTTGGGTG GGTTCGCAGT
    GAAGGGACTG ATGTCAGACT ACAGAGGGAA GTGGGACTTC CCTGGACTGA GCTGCGTACT
    CTTTCTGCTG CCTCAGCCCA GCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235869
    Clone ID Related Accession (Same CDS sequence) XM_010806811.3
    Accession Version XM_010806811.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5304bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-3(V) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806811.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCCGGAC GCAGGCCGAT CCTGTGGATG TGCTGAAGGC ACTGGGCATG
    CGGGGGGGCC AGGCTGGGAT CCCTGAGGGA CCCGGCCTCT GCCCCCAGAG GGTCCCGGAG
    GGCGACCGAG CATTCCGGGT GGGCAAGACT GGCACACTTG GTGTCCCCAC GCAGGAGCTC
    TTCCAAGATG GGCACTTTCC TGAGAACTTC TCCATCCTGA TCACCCTGCG GGGCCAGCCG
    GCCAACCGTT CTGTCCTTCT GTCCATCTAT GATGAGGGCG GCGTCCGGCA GCTCGGCTTG
    GCCTTGGGGC CAGCTCTGGA CCTCCTAGGT GACTCCTTCA GCCCCCTCCC CCAGCAGGTC
    AACCTCACGG ATGGCAGGTG GCACCGTGTG GCAGTCAGTG TGGACGGTGG GATGGTGACC
    CTGGTGGCTG ATTGTGAACC TCAGCTCCCC ATGTTGGCTC AGGGACCTCG ATTCATCAGT
    ACAGCGGGAC TCACTGTGCT GGGGACCCAG GACCTTGGCG AGGAGACTTT TGAGGGAGAT
    GTTCAGGAGC TGGTGATAAG CCCAGATCCC CAGGCTGCCT TCCAGGCATG TGAGCGGTAC
    CTTCCTGGCT GTGATGACCT GGATCCAACA GTCACCACGG TCCCTCAGGG GGAGCCAGAA
    ACCCCTCCCC CTAGGCGGAA GGGGAAAGGA AAAGGGAAGA AAAAAGGGCG AGGTCGTAAA
    GGGAAGGGCA GGAAGAAGAA GAACAAGGAA ACTGTGGCTG TGAGTCCTCC TCCTGGCTCC
    CTGGACAACC AGACCTCCAC TGACATCCCC AAGACAGAGA CACCAGAACC AACTCTGTCT
    CCGACACCCA CGCCTTTGGT CATCACCACA ACTGTGACCT TTGGTAGCAA TGTCACCATC
    CTAGAGGAGG GCCTGGACCC TGACAATGGA ACTGAACTGG AGACCCTGGA GACTGAGTTA
    GACAGAGAAG ATGAAGAAGA AGGTGATCCC ACCATGGGCC CCCACTTCCG GGCAGCAGAA
    CAGTCATGGA ATACTGAGTT CCAGATCTTT CCTGGTGCTG GGGAGAAAGG AGAAAAAGGA
    GAACCAGCTG TGGTCGAACA GGGACAGCAG TTTGAAGGAC CCCCAGGAGC CCCGGGACCC
    CAAGGGGTGG TTGGCCCCTC AGGCCCTCCT GGCCCCCCAG GATTCCCTGG AGACCGTGGT
    CTACCGGGCC CTGCTGGCCT CCCAGGCATC CCTGGCATGG ATGGGATCCG GGGTCTCCCA
    GGCACTGTGA TCATGATGCC GTTCCAGTTT GCAAGAGGCT CTCACAAAGG ACCCCCAGTA
    TCCTTCCAGC AGGCCCAGGC TCAGGCAGTC CTGCAACAAG CTCAGCTGTC TATGAAAGGC
    CCTCCTGGCC CGGTGGGGCT CACCGGACGC CCAGGTCCCG TGGGTCTTCC TGGGTATCCA
    GGTCTGAAAG GAGAGATGGG AGAAATGGGG CCGCAGGGTC CCCGAGGGCT TCAGGGACCT
    CATGGGCCCC CTGGCCGAGA GGGAAAGATG GGCCGCGCTG GAGCAGATGG GGCTCGGGGC
    CTCCCCGGCG CTACGGGACC TAAGGGTGAT CGGGGCTTTG ATGGCCTGCC AGGGCTGCCT
    GGTGAGAAGG GCCAAAGGGG TGACTTTGGT TATGTGGGGA AACCTGGTCC CCCAGGAGAG
    GATGGACGGA AGGGAGCAGA GGGACCTCCG GGGCCCACTG GCCAGGCAGG GGAGCCGGGC
    CCCCGAGGAC TGATTGGCCC TAGAGGCTCC CCCGGACCCC TGGGACGCCC GGGTGTGATT
    GGAAGTGATG GTGCTCCAGG TGCCAAAGGA AATGTGGGTC CTCCAGGAGA ACCAGGCCCC
    CCAGGACAGC AGGGAAATCC TGGGTCCCAG GGAATTCCCG GCCCCCAGGG ACCCATCGGC
    ACTCCTGGGG AGAAGGGTCC TCCTGGAAAC CCAGGAATTC CAGGCCTCCC AGGATCTGAA
    GGCCCTCCGG GTCACCCAGG TCATGAGGGC CCAACAGGAG AGAAAGGGGC CCAGGGTCCA
    GCAGGGTCAG CAGGCCCTCC GGGCTATCCA GGACCTCGGG GTGTGAAGGG TACCTCTGGC
    AACCGGGGCC TCCAGGGGGA GAAAGGAGAG AAGGGAGAGG ATGGTTTCCC AGGCTTCAAG
    GGTGATGCGG GGCTTAAAGG AGATCGGGGG CAACCCGGAC CTCCAGGTCC TCGGGGAGAG
    GATGGTCCGG AGGGGCTAAA GGGGCAGGAG GGGCTGCTTG GCGAGGAGGG TCCCCCAGGC
    TCAGCTGGGG AGAAGGGCAA GCTTGGCGTG CCGGGTCTCC CAGGTTATCC AGGACGTCCA
    GGCCCTAAGG GATCTATTGG CTTTCCGGGA CCTCTGGGAC CATTAGGAGA AAAAGGGAAG
    CGGGGGAAGG CTGGGCAGCC AGGCCTGGAA GGAGAGCGGG GACCACCAGG TGCCCGTGGA
    GAGAGAGGGC AACCAGGTGC CACAGGGCAG CCGGGCCCCA AGGGTGATGT GGGCCAGGAC
    GGAGCCCCTG GGTTCCCTGG AGAAAAGGGC CTCCCAGGTC TGCAAGGTCC TCCTGGGTTC
    TCTGGACCAA AGGGTCCCCC TGGTCCCCAG GGAAAAGATG GGGTACCTGG ACACCCTGGA
    CAGAGAGGAG AATTGGGTTT CCAAGGTCAG ACAGGCCCAC CTGGACCAGC TGGTGTCGTG
    GGTCCTCAGG GAAAGACAGG AGAAGCAGGG CCTCTGGGTG AGAGGGGGCC CCCAGGGCCC
    CCTGGACCTC CTGGTGAACA AGGTCTTCCG GGCCTGGAAG GCAGAGAGGG GGCCAAGGGG
    GATGTGGGAC CACTGGGACC CCTTGGGAAG GAAGGGCCAC CTGGACCCAG AGGTTTCCCT
    GGTCCTCATG GAGCCCCCGG GGACCCAGGA CCGACTGGTT TAAAGGGTGA CAAGGGCCCC
    CCAGGTCCTG TTGGTGCCAA CGGATCCCCT GGGGAGCGTG GTCCTGTAGG CCCAGCAGGG
    AGCATTGGGC TTCCTGGTCA AAGCGGAGGC CAAGGCCCCG TGGGCCCCAC AGGCGAGAAG
    GGTTCCCCGG GTGAACGTGG CGCCCCTGGC CCAACTGGTA AAGATGGGAT CCCCGGGCCC
    CTGGGGCTTC CTGGGCCCCC TGGAGCTGCG GGGCCTTCTG GCGAGGAAGG GGACAAGGGA
    GAAGTGGGTG CCCCCGGTCA CAAAGGCAGC AAAGGTGACA AGGGAGATGC GGGCCCGCCT
    GGACCAACAG GGATACGGGG TCTGGTGGGA CACCCCGGCT CCCCGGGAGC AGACGGGGCT
    CAGGGACGCC GGGGACCCCC AGGCCTCTTT GGGCAGAAAG GAGATGATGG AGTGAGAGGC
    TTCCCTGGCA TGATTGGTCC TCCTGGCCTA CAGGGAATGC CAGGCCCTGC TGGAGAAAAG
    GGAGAAGTTG GAGATGTAGG GTCCATGGGT CCCCACGGAG CTCCAGGCCC TCGGGGCCCC
    CAGGGCCCCA GCGGATCAGA GGGTCCTCCA GGGTTGCCTG GGGGAGTTGG TCAGCCTGGC
    GCTGTGGGGG AGAAGGGTGA GCCAGGAGAG CCTGGAGACC CAGGACCCCC CGGGACCCCA
    GGCACCCCGG GCCCCAGGGG AGAAGTTGGT GAAAAGGGGG ATACAGGCCC ATCTGGGGCT
    GCTGGACCCC CAGGCAAGAA AGGACCCCCT GGAGAGGATG GAGCAAAGGG GAATGTGGGC
    CCCACAGGCC TCCCAGGAGA CCTAGGCCCC CCCGGAGACC CTGGAGTTTC GGGTCAAGAT
    GGCCCCCCAG GGGAGAAGGG AAACCCTGGA GATGTGGGGG GACCGGGTCC ACCTGGCGCT
    TCTGGGGAGC CTGGCGCCCC TGGGCCTCCT GGTAAAAGGG GTCCTTCTGG TCGCATGGGT
    CAAGAAGGTA GAGAAGGAGA GAAAGGTGCC AAGGGGGAAC CAGGTCCTGA TGGACCCCCA
    GGGAGGACAG GCCCAGTAGG GGCTCGAGGG CCCCCTGGAC GTGTTGGACC TGAGGGTCTT
    CGTGGGATCC CTGGACCTGT GGGCGAACCA GGCCTCCTGG GACCTCCTGG ACTGTTAGGC
    CCTCCTGGTC CCCTGGGGCC CTCTGGCCTA CCAGGGCTGA AGGGAGATGC TGGCCCCAAG
    GGAGAAAAGG GCCACATTGG ATTAATTGGT CTTGTCGGCC CCCCTGGGGA GGTTGGTGAG
    AAAGGGGATC AGGGGTTGCC AGGAGTGCAA GGCTCCCCTG GCCTCCAGGG AGAACCGGGT
    TCTCCTGGCC CCATTGGCTC TCTGGGCCAC CCTGGGCCCC AAGGCGTGGT GGGTCCTCCA
    GGACAGAAAG GCTCAAAGGG GTCCCCGGGA TCCCAAGGTC CTCGTGGAGA CGCTGGCCCC
    CCAGGTCCTC CTGGTCCCCC GGGTCCCTCT GCGGAGCTGC ACGGGCTGCG CCGGCGCCGA
    CGTTCCCTGC TAGGCTTGGG AACCCCGGAG GGCGGCCTGG AGGAGGTGCT GGCCTCGATG
    GCGTCGCTGA GCTTCGAGTT AGAGCAGCTG CGGCGCCCTC CCGGCACGGC TGAGCGCCCG
    GGCCTCGTGT GCAAAGAGTT GCACCGCAGC CATCCGCATC TGCCAGATGG GGAATACTGG
    ATTGATCCCA ATCAAGGCTG CGCGCGGGAT GCCCTCAGGG TTTTCTGTAA CTTCACGGCA
    GGAGGAGAAA CCTGCCTCTA CCCAGACAAG AAGTTTGAGA CCGTGAAACT GGCCTCTTGG
    TCCAAAGAAA AGCCAGGTGG CTGGTATAGC ACATTCCGTC GAGGGAAAAA GTTCTCCTAC
    GTGGATGCCG ATGGGGCCGC AGTGAGCGTG GTACAGCTGA CCTTCCTGAA GTTGCTGAGT
    GCCACGGCCC GCCAGAGCTT CACATTCACC TGCCAGAACT CGGCCGCCTG GCTGGACGAA
    GCTGCAGGTG ACCACAGCCG CTCTGCCCGT TTCCTCGGGG CCAATGGGGA GGAGGTATCC
    TTCAACCAGA TGGCAGCCGC CACCATCACA GTCCCCTACG ATGGCTGCAG GCTCCGGAAG
    GGACAGTCGA AGACCCTCTT TGAGTTCAGC TCCTCCCGAG TGGGATTTCT GCCCCTGTTG
    GACGTGGCAG CTGCTGACTT TGGCCAGACG AACCAGAAGT TTGGGTGGGT TCGCAGTGAA
    GGGACTGATG TCAGACTACA GAGGGAAGTG GGACTTCCCT GGACTGAGCT GCGTACTCTT
    TCTGCTGCCT CAGCCCAGCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805113.2
    CDS157..5460
    Translation

    Target ORF information:

    RefSeq Version XM_010806811.3
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type V alpha 3 chain (COL5A3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806811.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCCGGAC GCAGGCCGAT CCTGTGGATG TGCTGAAGGC ACTGGGCATG
    CGGGGGGGCC AGGCTGGGAT CCCTGAGGGA CCCGGCCTCT GCCCCCAGAG GGTCCCGGAG
    GGCGACCGAG CATTCCGGGT GGGCAAGACT GGCACACTTG GTGTCCCCAC GCAGGAGCTC
    TTCCAAGATG GGCACTTTCC TGAGAACTTC TCCATCCTGA TCACCCTGCG GGGCCAGCCG
    GCCAACCGTT CTGTCCTTCT GTCCATCTAT GATGAGGGCG GCGTCCGGCA GCTCGGCTTG
    GCCTTGGGGC CAGCTCTGGA CCTCCTAGGT GACTCCTTCA GCCCCCTCCC CCAGCAGGTC
    AACCTCACGG ATGGCAGGTG GCACCGTGTG GCAGTCAGTG TGGACGGTGG GATGGTGACC
    CTGGTGGCTG ATTGTGAACC TCAGCTCCCC ATGTTGGCTC AGGGACCTCG ATTCATCAGT
    ACAGCGGGAC TCACTGTGCT GGGGACCCAG GACCTTGGCG AGGAGACTTT TGAGGGAGAT
    GTTCAGGAGC TGGTGATAAG CCCAGATCCC CAGGCTGCCT TCCAGGCATG TGAGCGGTAC
    CTTCCTGGCT GTGATGACCT GGATCCAACA GTCACCACGG TCCCTCAGGG GGAGCCAGAA
    ACCCCTCCCC CTAGGCGGAA GGGGAAAGGA AAAGGGAAGA AAAAAGGGCG AGGTCGTAAA
    GGGAAGGGCA GGAAGAAGAA GAACAAGGAA ACTGTGGCTG TGAGTCCTCC TCCTGGCTCC
    CTGGACAACC AGACCTCCAC TGACATCCCC AAGACAGAGA CACCAGAACC AACTCTGTCT
    CCGACACCCA CGCCTTTGGT CATCACCACA ACTGTGACCT TTGGTAGCAA TGTCACCATC
    CTAGAGGAGG GCCTGGACCC TGACAATGGA ACTGAACTGG AGACCCTGGA GACTGAGTTA
    GACAGAGAAG ATGAAGAAGA AGGTGATCCC ACCATGGGCC CCCACTTCCG GGCAGCAGAA
    CAGTCATGGA ATACTGAGTT CCAGATCTTT CCTGGTGCTG GGGAGAAAGG AGAAAAAGGA
    GAACCAGCTG TGGTCGAACA GGGACAGCAG TTTGAAGGAC CCCCAGGAGC CCCGGGACCC
    CAAGGGGTGG TTGGCCCCTC AGGCCCTCCT GGCCCCCCAG GATTCCCTGG AGACCGTGGT
    CTACCGGGCC CTGCTGGCCT CCCAGGCATC CCTGGCATGG ATGGGATCCG GGGTCTCCCA
    GGCACTGTGA TCATGATGCC GTTCCAGTTT GCAAGAGGCT CTCACAAAGG ACCCCCAGTA
    TCCTTCCAGC AGGCCCAGGC TCAGGCAGTC CTGCAACAAG CTCAGCTGTC TATGAAAGGC
    CCTCCTGGCC CGGTGGGGCT CACCGGACGC CCAGGTCCCG TGGGTCTTCC TGGGTATCCA
    GGTCTGAAAG GAGAGATGGG AGAAATGGGG CCGCAGGGTC CCCGAGGGCT TCAGGGACCT
    CATGGGCCCC CTGGCCGAGA GGGAAAGATG GGCCGCGCTG GAGCAGATGG GGCTCGGGGC
    CTCCCCGGCG CTACGGGACC TAAGGGTGAT CGGGGCTTTG ATGGCCTGCC AGGGCTGCCT
    GGTGAGAAGG GCCAAAGGGG TGACTTTGGT TATGTGGGGA AACCTGGTCC CCCAGGAGAG
    GATGGACGGA AGGGAGCAGA GGGACCTCCG GGGCCCACTG GCCAGGCAGG GGAGCCGGGC
    CCCCGAGGAC TGATTGGCCC TAGAGGCTCC CCCGGACCCC TGGGACGCCC GGGTGTGATT
    GGAAGTGATG GTGCTCCAGG TGCCAAAGGA AATGTGGGTC CTCCAGGAGA ACCAGGCCCC
    CCAGGACAGC AGGGAAATCC TGGGTCCCAG GGAATTCCCG GCCCCCAGGG ACCCATCGGC
    ACTCCTGGGG AGAAGGGTCC TCCTGGAAAC CCAGGAATTC CAGGCCTCCC AGGATCTGAA
    GGCCCTCCGG GTCACCCAGG TCATGAGGGC CCAACAGGAG AGAAAGGGGC CCAGGGTCCA
    GCAGGGTCAG CAGGCCCTCC GGGCTATCCA GGACCTCGGG GTGTGAAGGG TACCTCTGGC
    AACCGGGGCC TCCAGGGGGA GAAAGGAGAG AAGGGAGAGG ATGGTTTCCC AGGCTTCAAG
    GGTGATGCGG GGCTTAAAGG AGATCGGGGG CAACCCGGAC CTCCAGGTCC TCGGGGAGAG
    GATGGTCCGG AGGGGCTAAA GGGGCAGGAG GGGCTGCTTG GCGAGGAGGG TCCCCCAGGC
    TCAGCTGGGG AGAAGGGCAA GCTTGGCGTG CCGGGTCTCC CAGGTTATCC AGGACGTCCA
    GGCCCTAAGG GATCTATTGG CTTTCCGGGA CCTCTGGGAC CATTAGGAGA AAAAGGGAAG
    CGGGGGAAGG CTGGGCAGCC AGGCCTGGAA GGAGAGCGGG GACCACCAGG TGCCCGTGGA
    GAGAGAGGGC AACCAGGTGC CACAGGGCAG CCGGGCCCCA AGGGTGATGT GGGCCAGGAC
    GGAGCCCCTG GGTTCCCTGG AGAAAAGGGC CTCCCAGGTC TGCAAGGTCC TCCTGGGTTC
    TCTGGACCAA AGGGTCCCCC TGGTCCCCAG GGAAAAGATG GGGTACCTGG ACACCCTGGA
    CAGAGAGGAG AATTGGGTTT CCAAGGTCAG ACAGGCCCAC CTGGACCAGC TGGTGTCGTG
    GGTCCTCAGG GAAAGACAGG AGAAGCAGGG CCTCTGGGTG AGAGGGGGCC CCCAGGGCCC
    CCTGGACCTC CTGGTGAACA AGGTCTTCCG GGCCTGGAAG GCAGAGAGGG GGCCAAGGGG
    GATGTGGGAC CACTGGGACC CCTTGGGAAG GAAGGGCCAC CTGGACCCAG AGGTTTCCCT
    GGTCCTCATG GAGCCCCCGG GGACCCAGGA CCGACTGGTT TAAAGGGTGA CAAGGGCCCC
    CCAGGTCCTG TTGGTGCCAA CGGATCCCCT GGGGAGCGTG GTCCTGTAGG CCCAGCAGGG
    AGCATTGGGC TTCCTGGTCA AAGCGGAGGC CAAGGCCCCG TGGGCCCCAC AGGCGAGAAG
    GGTTCCCCGG GTGAACGTGG CGCCCCTGGC CCAACTGGTA AAGATGGGAT CCCCGGGCCC
    CTGGGGCTTC CTGGGCCCCC TGGAGCTGCG GGGCCTTCTG GCGAGGAAGG GGACAAGGGA
    GAAGTGGGTG CCCCCGGTCA CAAAGGCAGC AAAGGTGACA AGGGAGATGC GGGCCCGCCT
    GGACCAACAG GGATACGGGG TCTGGTGGGA CACCCCGGCT CCCCGGGAGC AGACGGGGCT
    CAGGGACGCC GGGGACCCCC AGGCCTCTTT GGGCAGAAAG GAGATGATGG AGTGAGAGGC
    TTCCCTGGCA TGATTGGTCC TCCTGGCCTA CAGGGAATGC CAGGCCCTGC TGGAGAAAAG
    GGAGAAGTTG GAGATGTAGG GTCCATGGGT CCCCACGGAG CTCCAGGCCC TCGGGGCCCC
    CAGGGCCCCA GCGGATCAGA GGGTCCTCCA GGGTTGCCTG GGGGAGTTGG TCAGCCTGGC
    GCTGTGGGGG AGAAGGGTGA GCCAGGAGAG CCTGGAGACC CAGGACCCCC CGGGACCCCA
    GGCACCCCGG GCCCCAGGGG AGAAGTTGGT GAAAAGGGGG ATACAGGCCC ATCTGGGGCT
    GCTGGACCCC CAGGCAAGAA AGGACCCCCT GGAGAGGATG GAGCAAAGGG GAATGTGGGC
    CCCACAGGCC TCCCAGGAGA CCTAGGCCCC CCCGGAGACC CTGGAGTTTC GGGTCAAGAT
    GGCCCCCCAG GGGAGAAGGG AAACCCTGGA GATGTGGGGG GACCGGGTCC ACCTGGCGCT
    TCTGGGGAGC CTGGCGCCCC TGGGCCTCCT GGTAAAAGGG GTCCTTCTGG TCGCATGGGT
    CAAGAAGGTA GAGAAGGAGA GAAAGGTGCC AAGGGGGAAC CAGGTCCTGA TGGACCCCCA
    GGGAGGACAG GCCCAGTAGG GGCTCGAGGG CCCCCTGGAC GTGTTGGACC TGAGGGTCTT
    CGTGGGATCC CTGGACCTGT GGGCGAACCA GGCCTCCTGG GACCTCCTGG ACTGTTAGGC
    CCTCCTGGTC CCCTGGGGCC CTCTGGCCTA CCAGGGCTGA AGGGAGATGC TGGCCCCAAG
    GGAGAAAAGG GCCACATTGG ATTAATTGGT CTTGTCGGCC CCCCTGGGGA GGTTGGTGAG
    AAAGGGGATC AGGGGTTGCC AGGAGTGCAA GGCTCCCCTG GCCTCCAGGG AGAACCGGGT
    TCTCCTGGCC CCATTGGCTC TCTGGGCCAC CCTGGGCCCC AAGGCGTGGT GGGTCCTCCA
    GGACAGAAAG GCTCAAAGGG GTCCCCGGGA TCCCAAGGTC CTCGTGGAGA CGCTGGCCCC
    CCAGGTCCTC CTGGTCCCCC GGGTCCCTCT GCGGAGCTGC ACGGGCTGCG CCGGCGCCGA
    CGTTCCCTGC TAGGCTTGGG AACCCCGGAG GGCGGCCTGG AGGAGGTGCT GGCCTCGATG
    GCGTCGCTGA GCTTCGAGTT AGAGCAGCTG CGGCGCCCTC CCGGCACGGC TGAGCGCCCG
    GGCCTCGTGT GCAAAGAGTT GCACCGCAGC CATCCGCATC TGCCAGATGG GGAATACTGG
    ATTGATCCCA ATCAAGGCTG CGCGCGGGAT GCCCTCAGGG TTTTCTGTAA CTTCACGGCA
    GGAGGAGAAA CCTGCCTCTA CCCAGACAAG AAGTTTGAGA CCGTGAAACT GGCCTCTTGG
    TCCAAAGAAA AGCCAGGTGG CTGGTATAGC ACATTCCGTC GAGGGAAAAA GTTCTCCTAC
    GTGGATGCCG ATGGGGCCGC AGTGAGCGTG GTACAGCTGA CCTTCCTGAA GTTGCTGAGT
    GCCACGGCCC GCCAGAGCTT CACATTCACC TGCCAGAACT CGGCCGCCTG GCTGGACGAA
    GCTGCAGGTG ACCACAGCCG CTCTGCCCGT TTCCTCGGGG CCAATGGGGA GGAGGTATCC
    TTCAACCAGA TGGCAGCCGC CACCATCACA GTCCCCTACG ATGGCTGCAG GCTCCGGAAG
    GGACAGTCGA AGACCCTCTT TGAGTTCAGC TCCTCCCGAG TGGGATTTCT GCCCCTGTTG
    GACGTGGCAG CTGCTGACTT TGGCCAGACG AACCAGAAGT TTGGGTGGGT TCGCAGTGAA
    GGGACTGATG TCAGACTACA GAGGGAAGTG GGACTTCCCT GGACTGAGCT GCGTACTCTT
    TCTGCTGCCT CAGCCCAGCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235870
    Clone ID Related Accession (Same CDS sequence) XM_010806813.3
    Accession Version XM_010806813.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5244bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-3(V) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806813.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCCGGAC GCAGGCCGTA GATCCTGTGG ATGTGCTGAA GGCACTGGGC
    ATGCGGGGGG GCCAGGCTGG GATCCCTGAG GGACCCGGCC TCTGCCCCCA GAGGGTCCCG
    GAGGGCGACC GAGCATTCCG GGTGGGCAAG ACTGGCACAC TTGGTGTCCC CACGCAGGAG
    CTCTTCCAAG ATGGGCACTT TCCTGAGAAC TTCTCCATCC TGATCACCCT GCGGGGCCAG
    CCGGCCAACC GTTCTGTCCT TCTGTCCATC TATGATGAGG GCGGCGTCCG GCAGCTCGGC
    TTGGCCTTGG GGCCAGCTCT GGACCTCCTA GGTGACTCCT TCAGCCCCCT CCCCCAGCAG
    GTCAACCTCA CGGATGGCAG GTGGCACCGT GTGGCAGTCA GTGTGGACGG TGGGATGGTG
    ACCCTGGTGG CTGATTGTGA ACCTCAGCTC CCCATGTTGG CTCAGGGACC TCGATTCATC
    AGTACAGCGG GACTCACTGT GCTGGGGACC CAGGACCTTG GCGAGGAGAC TTTTGAGGGA
    GATGTTCAGG AGCTGGTGAT AAGCCCAGAT CCCCAGGCTG CCTTCCAGGC ATGTGAGCGG
    TACCTTCCTG GCTGTGATGA CCTGGATCCA ACAGTCACCA CGGTCCCTCA GGGGGAGCCA
    GAAACCCCTC CCCCTAGGCG GAAGGGGAAA GGAAAAGGGA AGAAAAAAGG GCGAGGTCGT
    AAAGGGAAGG GCAGGAAGAA GAAGAACAAG GAAACTGTGG CTGTGAGTCC TCCTCCTGGC
    TCCCTGGACA ACCAGACCTC CACTGACATC CCCAAGACAG AGACACCAGA ACCAACTCTG
    TCTCCGACAC CCACGCCTTT GGTCATCACC ACAACTGTGA CCTTTGGTAG CAATGTCACC
    ATCCTAGAGG AGGGCCTGGA CCCTGACAAT GGAACTGAAC TGGAGACCCT GGAGACTGAG
    TTAGACAGAG AAGATGAAGA AGAAGGTGAT CCCACCATGG GCCCCCACTT CCGGGCAGCA
    GAACAGTCAT GGAATACTGA GTTCCAGATC TTTCCTGGTG CTGGGGAGAA AGGAGAAAAA
    GGAGAACCAG CTGTGGTCGA ACAGGGACAG CAGTTTGAAG GACCCCCAGG AGCCCCGGGA
    CCCCAAGGCC CTGCTGGCCT CCCAGGCATC CCTGGCATGG ATGGGATCCG GGGTCTCCCA
    GGCACTGTGA TCATGATGCC GTTCCAGTTT GCAAGAGGCT CTCACAAAGG ACCCCCAGTA
    TCCTTCCAGC AGGCCCAGGC TCAGGCAGTC CTGCAACAAG CTCAGCTGTC TATGAAAGGC
    CCTCCTGGCC CGGTGGGGCT CACCGGACGC CCAGGTCCCG TGGGTCTTCC TGGGTATCCA
    GGTCTGAAAG GAGAGATGGG AGAAATGGGG CCGCAGGGTC CCCGAGGGCT TCAGGGACCT
    CATGGGCCCC CTGGCCGAGA GGGAAAGATG GGCCGCGCTG GAGCAGATGG GGCTCGGGGC
    CTCCCCGGCG CTACGGGACC TAAGGGTGAT CGGGGCTTTG ATGGCCTGCC AGGGCTGCCT
    GGTGAGAAGG GCCAAAGGGG TGACTTTGGT TATGTGGGGA AACCTGGTCC CCCAGGAGAG
    GATGGACGGA AGGGAGCAGA GGGACCTCCG GGGCCCACTG GCCAGGCAGG GGAGCCGGGC
    CCCCGAGGAC TGATTGGCCC TAGAGGCTCC CCCGGACCCC TGGGACGCCC GGGTGTGATT
    GGAAGTGATG GTGCTCCAGG TGCCAAAGGA AATGTGGGTC CTCCAGGAGA ACCAGGCCCC
    CCAGGACAGC AGGGAAATCC TGGGTCCCAG GGAATTCCCG GCCCCCAGGG ACCCATCGGC
    ACTCCTGGGG AGAAGGGTCC TCCTGGAAAC CCAGGAATTC CAGGCCTCCC AGGATCTGAA
    GGCCCTCCGG GTCACCCAGG TCATGAGGGC CCAACAGGAG AGAAAGGGGC CCAGGGTCCA
    GCAGGGTCAG CAGGCCCTCC GGGCTATCCA GGACCTCGGG GTGTGAAGGG TACCTCTGGC
    AACCGGGGCC TCCAGGGGGA GAAAGGAGAG AAGGGAGAGG ATGGTTTCCC AGGCTTCAAG
    GGTGATGCGG GGCTTAAAGG AGATCGGGGG CAACCCGGAC CTCCAGGTCC TCGGGGAGAG
    GATGGTCCGG AGGGGCTAAA GGGGCAGGAG GGGCTGCTTG GCGAGGAGGG TCCCCCAGGC
    TCAGCTGGGG AGAAGGGCAA GCTTGGCGTG CCGGGTCTCC CAGGTTATCC AGGACGTCCA
    GGCCCTAAGG GATCTATTGG CTTTCCGGGA CCTCTGGGAC CATTAGGAGA AAAAGGGAAG
    CGGGGGAAGG CTGGGCAGCC AGGCCTGGAA GGAGAGCGGG GACCACCAGG TGCCCGTGGA
    GAGAGAGGGC AACCAGGTGC CACAGGGCAG CCGGGCCCCA AGGGTGATGT GGGCCAGGAC
    GGAGCCCCTG GGTTCCCTGG AGAAAAGGGC CTCCCAGGTC TGCAAGGTCC TCCTGGGTTC
    TCTGGACCAA AGGGTCCCCC TGGTCCCCAG GGAAAAGATG GGGTACCTGG ACACCCTGGA
    CAGAGAGGAG AATTGGGTTT CCAAGGTCAG ACAGGCCCAC CTGGACCAGC TGGTGTCGTG
    GGTCCTCAGG GAAAGACAGG AGAAGCAGGG CCTCTGGGTG AGAGGGGGCC CCCAGGGCCC
    CCTGGACCTC CTGGTGAACA AGGTCTTCCG GGCCTGGAAG GCAGAGAGGG GGCCAAGGGG
    GATGTGGGAC CACTGGGACC CCTTGGGAAG GAAGGGCCAC CTGGACCCAG AGGTTTCCCT
    GGTCCTCATG GAGCCCCCGG GGACCCAGGA CCGACTGGTT TAAAGGGTGA CAAGGGCCCC
    CCAGGTCCTG TTGGTGCCAA CGGATCCCCT GGGGAGCGTG GTCCTGTAGG CCCAGCAGGG
    AGCATTGGGC TTCCTGGTCA AAGCGGAGGC CAAGGCCCCG TGGGCCCCAC AGGCGAGAAG
    GGTTCCCCGG GTGAACGTGG CGCCCCTGGC CCAACTGGTA AAGATGGGAT CCCCGGGCCC
    CTGGGGCTTC CTGGGCCCCC TGGAGCTGCG GGGCCTTCTG GCGAGGAAGG GGACAAGGGA
    GAAGTGGGTG CCCCCGGTCA CAAAGGCAGC AAAGGTGACA AGGGAGATGC GGGCCCGCCT
    GGACCAACAG GGATACGGGG TCTGGTGGGA CACCCCGGCT CCCCGGGAGC AGACGGGGCT
    CAGGGACGCC GGGGACCCCC AGGCCTCTTT GGGCAGAAAG GAGATGATGG AGTGAGAGGC
    TTCCCTGGCA TGATTGGTCC TCCTGGCCTA CAGGGAATGC CAGGCCCTGC TGGAGAAAAG
    GGAGAAGTTG GAGATGTAGG GTCCATGGGT CCCCACGGAG CTCCAGGCCC TCGGGGCCCC
    CAGGGCCCCA GCGGATCAGA GGGTCCTCCA GGGTTGCCTG GGGGAGTTGG TCAGCCTGGC
    GCTGTGGGGG AGAAGGGTGA GCCAGGAGAG CCTGGAGACC CAGGACCCCC CGGGACCCCA
    GGCACCCCGG GCCCCAGGGG AGAAGTTGGT GAAAAGGGGG ATACAGGCCC ATCTGGGGCT
    GCTGGACCCC CAGGCAAGAA AGGACCCCCT GGAGAGGATG GAGCAAAGGG GAATGTGGGC
    CCCACAGGCC TCCCAGGAGA CCTAGGCCCC CCCGGAGACC CTGGAGTTTC GGGTCAAGAT
    GGCCCCCCAG GGGAGAAGGG AAACCCTGGA GATGTGGGGG GACCGGGTCC ACCTGGCGCT
    TCTGGGGAGC CTGGCGCCCC TGGGCCTCCT GGTAAAAGGG GTCCTTCTGG TCGCATGGGT
    CAAGAAGGTA GAGAAGGAGA GAAAGGTGCC AAGGGGGAAC CAGGTCCTGA TGGACCCCCA
    GGGAGGACAG GCCCAGTAGG GGCTCGAGGG CCCCCTGGAC GTGTTGGACC TGAGGGTCTT
    CGTGGGATCC CTGGACCTGT GGGCGAACCA GGCCTCCTGG GACCTCCTGG ACTGTTAGGC
    CCTCCTGGTC CCCTGGGGCC CTCTGGCCTA CCAGGGCTGA AGGGAGATGC TGGCCCCAAG
    GGAGAAAAGG GCCACATTGG ATTAATTGGT CTTGTCGGCC CCCCTGGGGA GGTTGGTGAG
    AAAGGGGATC AGGGGTTGCC AGGAGTGCAA GGCTCCCCTG GCCTCCAGGG AGAACCGGGT
    TCTCCTGGCC CCATTGGCTC TCTGGGCCAC CCTGGGCCCC AAGGCGTGGT GGGTCCTCCA
    GGACAGAAAG GCTCAAAGGG GTCCCCGGGA TCCCAAGGTC CTCGTGGAGA CGCTGGCCCC
    CCAGGTCCTC CTGGTCCCCC GGGTCCCTCT GCGGAGCTGC ACGGGCTGCG CCGGCGCCGA
    CGTTCCCTGC TAGGCTTGGG AACCCCGGAG GGCGGCCTGG AGGAGGTGCT GGCCTCGATG
    GCGTCGCTGA GCTTCGAGTT AGAGCAGCTG CGGCGCCCTC CCGGCACGGC TGAGCGCCCG
    GGCCTCGTGT GCAAAGAGTT GCACCGCAGC CATCCGCATC TGCCAGATGG GGAATACTGG
    ATTGATCCCA ATCAAGGCTG CGCGCGGGAT GCCCTCAGGG TTTTCTGTAA CTTCACGGCA
    GGAGGAGAAA CCTGCCTCTA CCCAGACAAG AAGTTTGAGA CCGTGAAACT GGCCTCTTGG
    TCCAAAGAAA AGCCAGGTGG CTGGTATAGC ACATTCCGTC GAGGGAAAAA GTTCTCCTAC
    GTGGATGCCG ATGGGGCCGC AGTGAGCGTG GTACAGCTGA CCTTCCTGAA GTTGCTGAGT
    GCCACGGCCC GCCAGAGCTT CACATTCACC TGCCAGAACT CGGCCGCCTG GCTGGACGAA
    GCTGCAGGTG ACCACAGCCG CTCTGCCCGT TTCCTCGGGG CCAATGGGGA GGAGGTATCC
    TTCAACCAGA TGGCAGCCGC CACCATCACA GTCCCCTACG ATGGCTGCAG GCTCCGGAAG
    GGACAGTCGA AGACCCTCTT TGAGTTCAGC TCCTCCCGAG TGGGATTTCT GCCCCTGTTG
    GACGTGGCAG CTGCTGACTT TGGCCAGACG AACCAGAAGT TTGGGTGGGT TCGCAGTGAA
    GGGACTGATG TCAGACTACA GAGGGAAGTG GGACTTCCCT GGACTGAGCT GCGTACTCTT
    TCTGCTGCCT CAGCCCAGCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805115.2
    CDS157..5400
    Translation

    Target ORF information:

    RefSeq Version XM_010806813.3
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type V alpha 3 chain (COL5A3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806813.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCCGGAC GCAGGCCGTA GATCCTGTGG ATGTGCTGAA GGCACTGGGC
    ATGCGGGGGG GCCAGGCTGG GATCCCTGAG GGACCCGGCC TCTGCCCCCA GAGGGTCCCG
    GAGGGCGACC GAGCATTCCG GGTGGGCAAG ACTGGCACAC TTGGTGTCCC CACGCAGGAG
    CTCTTCCAAG ATGGGCACTT TCCTGAGAAC TTCTCCATCC TGATCACCCT GCGGGGCCAG
    CCGGCCAACC GTTCTGTCCT TCTGTCCATC TATGATGAGG GCGGCGTCCG GCAGCTCGGC
    TTGGCCTTGG GGCCAGCTCT GGACCTCCTA GGTGACTCCT TCAGCCCCCT CCCCCAGCAG
    GTCAACCTCA CGGATGGCAG GTGGCACCGT GTGGCAGTCA GTGTGGACGG TGGGATGGTG
    ACCCTGGTGG CTGATTGTGA ACCTCAGCTC CCCATGTTGG CTCAGGGACC TCGATTCATC
    AGTACAGCGG GACTCACTGT GCTGGGGACC CAGGACCTTG GCGAGGAGAC TTTTGAGGGA
    GATGTTCAGG AGCTGGTGAT AAGCCCAGAT CCCCAGGCTG CCTTCCAGGC ATGTGAGCGG
    TACCTTCCTG GCTGTGATGA CCTGGATCCA ACAGTCACCA CGGTCCCTCA GGGGGAGCCA
    GAAACCCCTC CCCCTAGGCG GAAGGGGAAA GGAAAAGGGA AGAAAAAAGG GCGAGGTCGT
    AAAGGGAAGG GCAGGAAGAA GAAGAACAAG GAAACTGTGG CTGTGAGTCC TCCTCCTGGC
    TCCCTGGACA ACCAGACCTC CACTGACATC CCCAAGACAG AGACACCAGA ACCAACTCTG
    TCTCCGACAC CCACGCCTTT GGTCATCACC ACAACTGTGA CCTTTGGTAG CAATGTCACC
    ATCCTAGAGG AGGGCCTGGA CCCTGACAAT GGAACTGAAC TGGAGACCCT GGAGACTGAG
    TTAGACAGAG AAGATGAAGA AGAAGGTGAT CCCACCATGG GCCCCCACTT CCGGGCAGCA
    GAACAGTCAT GGAATACTGA GTTCCAGATC TTTCCTGGTG CTGGGGAGAA AGGAGAAAAA
    GGAGAACCAG CTGTGGTCGA ACAGGGACAG CAGTTTGAAG GACCCCCAGG AGCCCCGGGA
    CCCCAAGGCC CTGCTGGCCT CCCAGGCATC CCTGGCATGG ATGGGATCCG GGGTCTCCCA
    GGCACTGTGA TCATGATGCC GTTCCAGTTT GCAAGAGGCT CTCACAAAGG ACCCCCAGTA
    TCCTTCCAGC AGGCCCAGGC TCAGGCAGTC CTGCAACAAG CTCAGCTGTC TATGAAAGGC
    CCTCCTGGCC CGGTGGGGCT CACCGGACGC CCAGGTCCCG TGGGTCTTCC TGGGTATCCA
    GGTCTGAAAG GAGAGATGGG AGAAATGGGG CCGCAGGGTC CCCGAGGGCT TCAGGGACCT
    CATGGGCCCC CTGGCCGAGA GGGAAAGATG GGCCGCGCTG GAGCAGATGG GGCTCGGGGC
    CTCCCCGGCG CTACGGGACC TAAGGGTGAT CGGGGCTTTG ATGGCCTGCC AGGGCTGCCT
    GGTGAGAAGG GCCAAAGGGG TGACTTTGGT TATGTGGGGA AACCTGGTCC CCCAGGAGAG
    GATGGACGGA AGGGAGCAGA GGGACCTCCG GGGCCCACTG GCCAGGCAGG GGAGCCGGGC
    CCCCGAGGAC TGATTGGCCC TAGAGGCTCC CCCGGACCCC TGGGACGCCC GGGTGTGATT
    GGAAGTGATG GTGCTCCAGG TGCCAAAGGA AATGTGGGTC CTCCAGGAGA ACCAGGCCCC
    CCAGGACAGC AGGGAAATCC TGGGTCCCAG GGAATTCCCG GCCCCCAGGG ACCCATCGGC
    ACTCCTGGGG AGAAGGGTCC TCCTGGAAAC CCAGGAATTC CAGGCCTCCC AGGATCTGAA
    GGCCCTCCGG GTCACCCAGG TCATGAGGGC CCAACAGGAG AGAAAGGGGC CCAGGGTCCA
    GCAGGGTCAG CAGGCCCTCC GGGCTATCCA GGACCTCGGG GTGTGAAGGG TACCTCTGGC
    AACCGGGGCC TCCAGGGGGA GAAAGGAGAG AAGGGAGAGG ATGGTTTCCC AGGCTTCAAG
    GGTGATGCGG GGCTTAAAGG AGATCGGGGG CAACCCGGAC CTCCAGGTCC TCGGGGAGAG
    GATGGTCCGG AGGGGCTAAA GGGGCAGGAG GGGCTGCTTG GCGAGGAGGG TCCCCCAGGC
    TCAGCTGGGG AGAAGGGCAA GCTTGGCGTG CCGGGTCTCC CAGGTTATCC AGGACGTCCA
    GGCCCTAAGG GATCTATTGG CTTTCCGGGA CCTCTGGGAC CATTAGGAGA AAAAGGGAAG
    CGGGGGAAGG CTGGGCAGCC AGGCCTGGAA GGAGAGCGGG GACCACCAGG TGCCCGTGGA
    GAGAGAGGGC AACCAGGTGC CACAGGGCAG CCGGGCCCCA AGGGTGATGT GGGCCAGGAC
    GGAGCCCCTG GGTTCCCTGG AGAAAAGGGC CTCCCAGGTC TGCAAGGTCC TCCTGGGTTC
    TCTGGACCAA AGGGTCCCCC TGGTCCCCAG GGAAAAGATG GGGTACCTGG ACACCCTGGA
    CAGAGAGGAG AATTGGGTTT CCAAGGTCAG ACAGGCCCAC CTGGACCAGC TGGTGTCGTG
    GGTCCTCAGG GAAAGACAGG AGAAGCAGGG CCTCTGGGTG AGAGGGGGCC CCCAGGGCCC
    CCTGGACCTC CTGGTGAACA AGGTCTTCCG GGCCTGGAAG GCAGAGAGGG GGCCAAGGGG
    GATGTGGGAC CACTGGGACC CCTTGGGAAG GAAGGGCCAC CTGGACCCAG AGGTTTCCCT
    GGTCCTCATG GAGCCCCCGG GGACCCAGGA CCGACTGGTT TAAAGGGTGA CAAGGGCCCC
    CCAGGTCCTG TTGGTGCCAA CGGATCCCCT GGGGAGCGTG GTCCTGTAGG CCCAGCAGGG
    AGCATTGGGC TTCCTGGTCA AAGCGGAGGC CAAGGCCCCG TGGGCCCCAC AGGCGAGAAG
    GGTTCCCCGG GTGAACGTGG CGCCCCTGGC CCAACTGGTA AAGATGGGAT CCCCGGGCCC
    CTGGGGCTTC CTGGGCCCCC TGGAGCTGCG GGGCCTTCTG GCGAGGAAGG GGACAAGGGA
    GAAGTGGGTG CCCCCGGTCA CAAAGGCAGC AAAGGTGACA AGGGAGATGC GGGCCCGCCT
    GGACCAACAG GGATACGGGG TCTGGTGGGA CACCCCGGCT CCCCGGGAGC AGACGGGGCT
    CAGGGACGCC GGGGACCCCC AGGCCTCTTT GGGCAGAAAG GAGATGATGG AGTGAGAGGC
    TTCCCTGGCA TGATTGGTCC TCCTGGCCTA CAGGGAATGC CAGGCCCTGC TGGAGAAAAG
    GGAGAAGTTG GAGATGTAGG GTCCATGGGT CCCCACGGAG CTCCAGGCCC TCGGGGCCCC
    CAGGGCCCCA GCGGATCAGA GGGTCCTCCA GGGTTGCCTG GGGGAGTTGG TCAGCCTGGC
    GCTGTGGGGG AGAAGGGTGA GCCAGGAGAG CCTGGAGACC CAGGACCCCC CGGGACCCCA
    GGCACCCCGG GCCCCAGGGG AGAAGTTGGT GAAAAGGGGG ATACAGGCCC ATCTGGGGCT
    GCTGGACCCC CAGGCAAGAA AGGACCCCCT GGAGAGGATG GAGCAAAGGG GAATGTGGGC
    CCCACAGGCC TCCCAGGAGA CCTAGGCCCC CCCGGAGACC CTGGAGTTTC GGGTCAAGAT
    GGCCCCCCAG GGGAGAAGGG AAACCCTGGA GATGTGGGGG GACCGGGTCC ACCTGGCGCT
    TCTGGGGAGC CTGGCGCCCC TGGGCCTCCT GGTAAAAGGG GTCCTTCTGG TCGCATGGGT
    CAAGAAGGTA GAGAAGGAGA GAAAGGTGCC AAGGGGGAAC CAGGTCCTGA TGGACCCCCA
    GGGAGGACAG GCCCAGTAGG GGCTCGAGGG CCCCCTGGAC GTGTTGGACC TGAGGGTCTT
    CGTGGGATCC CTGGACCTGT GGGCGAACCA GGCCTCCTGG GACCTCCTGG ACTGTTAGGC
    CCTCCTGGTC CCCTGGGGCC CTCTGGCCTA CCAGGGCTGA AGGGAGATGC TGGCCCCAAG
    GGAGAAAAGG GCCACATTGG ATTAATTGGT CTTGTCGGCC CCCCTGGGGA GGTTGGTGAG
    AAAGGGGATC AGGGGTTGCC AGGAGTGCAA GGCTCCCCTG GCCTCCAGGG AGAACCGGGT
    TCTCCTGGCC CCATTGGCTC TCTGGGCCAC CCTGGGCCCC AAGGCGTGGT GGGTCCTCCA
    GGACAGAAAG GCTCAAAGGG GTCCCCGGGA TCCCAAGGTC CTCGTGGAGA CGCTGGCCCC
    CCAGGTCCTC CTGGTCCCCC GGGTCCCTCT GCGGAGCTGC ACGGGCTGCG CCGGCGCCGA
    CGTTCCCTGC TAGGCTTGGG AACCCCGGAG GGCGGCCTGG AGGAGGTGCT GGCCTCGATG
    GCGTCGCTGA GCTTCGAGTT AGAGCAGCTG CGGCGCCCTC CCGGCACGGC TGAGCGCCCG
    GGCCTCGTGT GCAAAGAGTT GCACCGCAGC CATCCGCATC TGCCAGATGG GGAATACTGG
    ATTGATCCCA ATCAAGGCTG CGCGCGGGAT GCCCTCAGGG TTTTCTGTAA CTTCACGGCA
    GGAGGAGAAA CCTGCCTCTA CCCAGACAAG AAGTTTGAGA CCGTGAAACT GGCCTCTTGG
    TCCAAAGAAA AGCCAGGTGG CTGGTATAGC ACATTCCGTC GAGGGAAAAA GTTCTCCTAC
    GTGGATGCCG ATGGGGCCGC AGTGAGCGTG GTACAGCTGA CCTTCCTGAA GTTGCTGAGT
    GCCACGGCCC GCCAGAGCTT CACATTCACC TGCCAGAACT CGGCCGCCTG GCTGGACGAA
    GCTGCAGGTG ACCACAGCCG CTCTGCCCGT TTCCTCGGGG CCAATGGGGA GGAGGTATCC
    TTCAACCAGA TGGCAGCCGC CACCATCACA GTCCCCTACG ATGGCTGCAG GCTCCGGAAG
    GGACAGTCGA AGACCCTCTT TGAGTTCAGC TCCTCCCGAG TGGGATTTCT GCCCCTGTTG
    GACGTGGCAG CTGCTGACTT TGGCCAGACG AACCAGAAGT TTGGGTGGGT TCGCAGTGAA
    GGGACTGATG TCAGACTACA GAGGGAAGTG GGACTTCCCT GGACTGAGCT GCGTACTCTT
    TCTGCTGCCT CAGCCCAGCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235871
    Clone ID Related Accession (Same CDS sequence) XM_005208735.4
    Accession Version XM_005208735.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-3(V) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005208735.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCCGGAC GCAGGCCGTA GATCCTGTGG ATGTGCTGAA GGCACTGGGC
    ATGCGGGGGG GCCAGGCTGG GATCCCTGAG GGACCCGGCC TCTGCCCCCA GAGGGTCCCG
    GAGGGCGACC GAGCATTCCG GGTGGGCAAG ACTGGCACAC TTGGTGTCCC CACGCAGGAG
    CTCTTCCAAG ATGGGCACTT TCCTGAGAAC TTCTCCATCC TGATCACCCT GCGGGGCCAG
    CCGGCCAACC GTTCTGTCCT TCTGTCCATC TATGATGAGG GCGGCGTCCG GCAGCTCGGC
    TTGGCCTTGG GGCCAGCTCT GGACCTCCTA GGTGACTCCT TCAGCCCCCT CCCCCAGCAG
    GTCAACCTCA CGGATGGCAG GTGGCACCGT GTGGCAGTCA GTGTGGACGG TGGGATGGTG
    ACCCTGGTGG CTGATTGTGA ACCTCAGCTC CCCATGTTGG CTCAGGGACC TCGATTCATC
    AGTACAGCGG GACTCACTGT GCTGGGGACC CAGGACCTTG GCGAGGAGAC TTTTGAGGGA
    GATGTTCAGG AGCTGGTGAT AAGCCCAGAT CCCCAGGCTG CCTTCCAGGC ATGTGAGCGG
    TACCTTCCTG GCTGTGATGA CCTGGATCCA ACAGTCACCA CGGTCCCTCA GGGGGAGCCA
    GAAACCCCTC CCCCTAGGCG GAAGGGGAAA GGAAAAGGGA AGAAAAAAGG GCGAGGTCGT
    AAAGGGAAGG GCAGGAAGAA GAAGAACAAG GAAACTGTGG CTGTGAGTCC TCCTCCTGGC
    TCCCTGGACA ACCAGACCTC CACTGACATC CCCAAGACAG AGACACCAGA ACCAACTCTG
    TCTCCGACAC CCACGCCTTT GGTCATCACC ACAACTGTGA CCTTTGGTAG CAATGTCACC
    ATCCTAGAGG AGGGCCTGGA CCCTGACAAT GGAACTGAAC TGGAGACCCT GGAGACTGAG
    TTAGACAGAG AAGATGAAGA AGAAGGTGAT CCCACCATGG GCCCCCACTT CCGGGCAGCA
    GAACAGTCAT GGAATACTGA GTTCCAGATC TTTCCTGGTG CTGGGGAGAA AGGAGAAAAA
    GGAGAACCAG CTGTGGTCGA ACAGGGACAG CAGTTTGAAG GACCCCCAGG AGCCCCGGGA
    CCCCAAGGGG TGGTTGGCCC CTCAGGCCCT CCTGGCCCCC CAGGATTCCC TGGAGACCGT
    GGTCTACCGG GCCCTGCTGG CCTCCCAGGC ATCCCTGGCA TGGATGGGAT CCGGGGTCTC
    CCAGGCACTG TGATCATGAT GCCGTTCCAG TTTGCAAGAG GCTCTCACAA AGGACCCCCA
    GTATCCTTCC AGCAGGCCCA GGCTCAGGCA GTCCTGCAAC AAGCTCAGCT GTCTATGAAA
    GGCCCTCCTG GCCCGGTGGG GCTCACCGGA CGCCCAGGTC CCGTGGGTCT TCCTGGGTAT
    CCAGGTCTGA AAGGAGAGAT GGGAGAAATG GGGCCGCAGG GTCCCCGAGG GCTTCAGGGA
    CCTCATGGGC CCCCTGGCCG AGAGGGAAAG ATGGGCCGCG CTGGAGCAGA TGGGGCTCGG
    GGCCTCCCCG GCGCTACGGG ACCTAAGGGT GATCGGGGCT TTGATGGCCT GCCAGGGCTG
    CCTGGTGAGA AGGGCCAAAG GGGTGACTTT GGTTATGTGG GGAAACCTGG TCCCCCAGGA
    GAGGATGGAC GGAAGGGAGC AGAGGGACCT CCGGGGCCCA CTGGCCAGGC AGGGGAGCCG
    GGCCCCCGAG GACTGATTGG CCCTAGAGGC TCCCCCGGAC CCCTGGGACG CCCGGGTGTG
    ATTGGAAGTG ATGGTGCTCC AGGTGCCAAA GGAAATGTGG GTCCTCCAGG AGAACCAGGC
    CCCCCAGGAC AGCAGGGAAA TCCTGGGTCC CAGGGAATTC CCGGCCCCCA GGGACCCATC
    GGCACTCCTG GGGAGAAGGG TCCTCCTGGA AACCCAGGAA TTCCAGGCCT CCCAGGATCT
    GAAGGCCCTC CGGGTCACCC AGGTCATGAG GGCCCAACAG GAGAGAAAGG GGCCCAGGGT
    CCAGCAGGGT CAGCAGGCCC TCCGGGCTAT CCAGGACCTC GGGGTGTGAA GGGTACCTCT
    GGCAACCGGG GCCTCCAGGG GGAGAAAGGA GAGAAGGGAG AGGATGGTTT CCCAGGCTTC
    AAGGGTGATG CGGGGCTTAA AGGAGATCGG GGGCAACCCG GACCTCCAGG TCCTCGGGGA
    GAGGATGGTC CGGAGGGGCT AAAGGGGCAG GAGGGGCTGC TTGGCGAGGA GGGTCCCCCA
    GGCTCAGCTG GGGAGAAGGG CAAGCTTGGC GTGCCGGGTC TCCCAGGTTA TCCAGGACGT
    CCAGGCCCTA AGGGATCTAT TGGCTTTCCG GGACCTCTGG GACCATTAGG AGAAAAAGGG
    AAGCGGGGGA AGGCTGGGCA GCCAGGCCTG GAAGGAGAGC GGGGACCACC AGGTGCCCGT
    GGAGAGAGAG GGCAACCAGG TGCCACAGGG CAGCCGGGCC CCAAGGGTGA TGTGGGCCAG
    GACGGAGCCC CTGGGTTCCC TGGAGAAAAG GGCCTCCCAG GTCTGCAAGG TCCTCCTGGG
    TTCTCTGGAC CAAAGGGTCC CCCTGGTCCC CAGGGAAAAG ATGGGGTACC TGGACACCCT
    GGACAGAGAG GAGAATTGGG TTTCCAAGGT CAGACAGGCC CACCTGGACC AGCTGGTGTC
    GTGGGTCCTC AGGGAAAGAC AGGAGAAGCA GGGCCTCTGG GTGAGAGGGG GCCCCCAGGG
    CCCCCTGGAC CTCCTGGTGA ACAAGGTCTT CCGGGCCTGG AAGGCAGAGA GGGGGCCAAG
    GGGGATGTGG GACCACTGGG ACCCCTTGGG AAGGAAGGGC CACCTGGACC CAGAGGTTTC
    CCTGGTCCTC ATGGAGCCCC CGGGGACCCA GGACCGACTG GTTTAAAGGG TGACAAGGGC
    CCCCCAGGTC CTGTTGGTGC CAACGGATCC CCTGGGGAGC GTGGTCCTGT AGGCCCAGCA
    GGGAGCATTG GGCTTCCTGG TCAAAGCGGA GGCCAAGGCC CCGTGGGCCC CACAGGCGAG
    AAGGGTTCCC CGGGTGAACG TGGCGCCCCT GGCCCAACTG GTAAAGATGG GATCCCCGGG
    CCCCTGGGGC TTCCTGGGCC CCCTGGAGCT GCGGGGCCTT CTGGCGAGGA AGGGGACAAG
    GGAGAAGTGG GTGCCCCCGG TCACAAAGGC AGCAAAGGTG ACAAGGGAGA TGCGGGCCCG
    CCTGGACCAA CAGGGATACG GGGTCTGGTG GGACACCCCG GCTCCCCGGG AGCAGACGGG
    GCTCAGGGAC GCCGGGGACC CCCAGGCCTC TTTGGGCAGA AAGGAGATGA TGGAGTGAGA
    GGCTTCCCTG GCATGATTGG TCCTCCTGGC CTACAGGGAA TGCCAGGCCC TGCTGGAGAA
    AAGGGAGAAG TTGGAGATGT AGGGTCCATG GGTCCCCACG GAGCTCCAGG CCCTCGGGGC
    CCCCAGGGCC CCAGCGGATC AGAGGGTCCT CCAGGGTTGC CTGGGGGAGT TGGTCAGCCT
    GGCGCTGTGG GGGAGAAGGG TGAGCCAGGA GAGCCTGGAG ACCCAGGACC CCCCGGGACC
    CCAGGCACCC CGGGCCCCAG GGGAGAAGTT GGTGAAAAGG GGGATACAGG CCCATCTGGG
    GCTGCTGGAC CCCCAGGCAA GAAAGGACCC CCTGGAGAGG ATGGAGCAAA GGGGAATGTG
    GGCCCCACAG GCCTCCCAGG AGACCTAGGC CCCCCCGGAG ACCCTGGAGT TTCGGGTCAA
    GATGGCCCCC CAGGGGAGAA GGGAAACCCT GGAGATGTGG GGGGACCGGG TCCACCTGGC
    GCTTCTGGGG AGCCTGGCGC CCCTGGGCCT CCTGGTAAAA GGGGTCCTTC TGGTCGCATG
    GGTCAAGAAG GTAGAGAAGG AGAGAAAGGT GCCAAGGGGG AACCAGGTCC TGATGGACCC
    CCAGGGAGGA CAGGCCCAGT AGGGGCTCGA GGGCCCCCTG GACGTGTTGG ACCTGAGGGT
    CTTCGTGGGA TCCCTGGACC TGTGGGCGAA CCAGGCCTCC TGGGACCTCC TGGACTGTTA
    GGCCCTCCTG GTCCCCTGGG GCCCTCTGGC CTACCAGGGC TGAAGGGAGA TGCTGGCCCC
    AAGGGAGAAA AGGGCCACAT TGGATTAATT GGTCTTGTCG GCCCCCCTGG GGAGGTTGGT
    GAGAAAGGGG ATCAGGGGTT GCCAGGAGTG CAAGGCTCCC CTGGCCTCCA GGGAGAACCG
    GGTTCTCCTG GCCCCATTGG CTCTCTGGGC CACCCTGGGC CCCAAGGCGT GGTGGGTCCT
    CCAGGACAGA AAGGCTCAAA GGGGTCCCCG GGATCCCAAG GTCCTCGTGG AGACGCTGGC
    CCCCCAGGTC CTCCTGGTCC CCCGGGTCCC TCTGCGGAGC TGCACGGGCT GCGCCGGCGC
    CGACGTTCCC TGCTAGGCTT GGGAACCCCG GAGGGCGGCC TGGAGGAGGT GCTGGCCTCG
    ATGGCGTCGC TGAGCTTCGA GTTAGAGCAG CTGCGGCGCC CTCCCGGCAC GGCTGAGCGC
    CCGGGCCTCG TGTGCAAAGA GTTGCACCGC AGCCATCCGC ATCTGCCAGA TGGGGAATAC
    TGGATTGATC CCAATCAAGG CTGCGCGCGG GATGCCCTCA GGGTTTTCTG TAACTTCACG
    GCAGGAGGAG AAACCTGCCT CTACCCAGAC AAGAAGTTTG AGACCGTGAA ACTGGCCTCT
    TGGTCCAAAG AAAAGCCAGG TGGCTGGTAT AGCACATTCC GTCGAGGGAA AAAGTTCTCC
    TACGTGGATG CCGATGGGGC CGCAGTGAGC GTGGTACAGC TGACCTTCCT GAAGTTGCTG
    AGTGCCACGG CCCGCCAGAG CTTCACATTC ACCTGCCAGA ACTCGGCCGC CTGGCTGGAC
    GAAGCTGCAG GTGACCACAG CCGCTCTGCC CGTTTCCTCG GGGCCAATGG GGAGGAGGTA
    TCCTTCAACC AGATGGCAGC CGCCACCATC ACAGTCCCCT ACGATGGCTG CAGGCTCCGG
    AAGGGACAGT CGAAGACCCT CTTTGAGTTC AGCTCCTCCC GAGTGGGATT TCTGCCCCTG
    TTGGACGTGG CAGCTGCTGA CTTTGGCCAG ACGAACCAGA AGTTTGGGTT TGAACTGGGC
    CCTGTCTGCT TTAGCAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005208792.2
    CDS157..5397
    Translation

    Target ORF information:

    RefSeq Version XM_005208735.4
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type V alpha 3 chain (COL5A3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005208735.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCCGGAC GCAGGCCGTA GATCCTGTGG ATGTGCTGAA GGCACTGGGC
    ATGCGGGGGG GCCAGGCTGG GATCCCTGAG GGACCCGGCC TCTGCCCCCA GAGGGTCCCG
    GAGGGCGACC GAGCATTCCG GGTGGGCAAG ACTGGCACAC TTGGTGTCCC CACGCAGGAG
    CTCTTCCAAG ATGGGCACTT TCCTGAGAAC TTCTCCATCC TGATCACCCT GCGGGGCCAG
    CCGGCCAACC GTTCTGTCCT TCTGTCCATC TATGATGAGG GCGGCGTCCG GCAGCTCGGC
    TTGGCCTTGG GGCCAGCTCT GGACCTCCTA GGTGACTCCT TCAGCCCCCT CCCCCAGCAG
    GTCAACCTCA CGGATGGCAG GTGGCACCGT GTGGCAGTCA GTGTGGACGG TGGGATGGTG
    ACCCTGGTGG CTGATTGTGA ACCTCAGCTC CCCATGTTGG CTCAGGGACC TCGATTCATC
    AGTACAGCGG GACTCACTGT GCTGGGGACC CAGGACCTTG GCGAGGAGAC TTTTGAGGGA
    GATGTTCAGG AGCTGGTGAT AAGCCCAGAT CCCCAGGCTG CCTTCCAGGC ATGTGAGCGG
    TACCTTCCTG GCTGTGATGA CCTGGATCCA ACAGTCACCA CGGTCCCTCA GGGGGAGCCA
    GAAACCCCTC CCCCTAGGCG GAAGGGGAAA GGAAAAGGGA AGAAAAAAGG GCGAGGTCGT
    AAAGGGAAGG GCAGGAAGAA GAAGAACAAG GAAACTGTGG CTGTGAGTCC TCCTCCTGGC
    TCCCTGGACA ACCAGACCTC CACTGACATC CCCAAGACAG AGACACCAGA ACCAACTCTG
    TCTCCGACAC CCACGCCTTT GGTCATCACC ACAACTGTGA CCTTTGGTAG CAATGTCACC
    ATCCTAGAGG AGGGCCTGGA CCCTGACAAT GGAACTGAAC TGGAGACCCT GGAGACTGAG
    TTAGACAGAG AAGATGAAGA AGAAGGTGAT CCCACCATGG GCCCCCACTT CCGGGCAGCA
    GAACAGTCAT GGAATACTGA GTTCCAGATC TTTCCTGGTG CTGGGGAGAA AGGAGAAAAA
    GGAGAACCAG CTGTGGTCGA ACAGGGACAG CAGTTTGAAG GACCCCCAGG AGCCCCGGGA
    CCCCAAGGGG TGGTTGGCCC CTCAGGCCCT CCTGGCCCCC CAGGATTCCC TGGAGACCGT
    GGTCTACCGG GCCCTGCTGG CCTCCCAGGC ATCCCTGGCA TGGATGGGAT CCGGGGTCTC
    CCAGGCACTG TGATCATGAT GCCGTTCCAG TTTGCAAGAG GCTCTCACAA AGGACCCCCA
    GTATCCTTCC AGCAGGCCCA GGCTCAGGCA GTCCTGCAAC AAGCTCAGCT GTCTATGAAA
    GGCCCTCCTG GCCCGGTGGG GCTCACCGGA CGCCCAGGTC CCGTGGGTCT TCCTGGGTAT
    CCAGGTCTGA AAGGAGAGAT GGGAGAAATG GGGCCGCAGG GTCCCCGAGG GCTTCAGGGA
    CCTCATGGGC CCCCTGGCCG AGAGGGAAAG ATGGGCCGCG CTGGAGCAGA TGGGGCTCGG
    GGCCTCCCCG GCGCTACGGG ACCTAAGGGT GATCGGGGCT TTGATGGCCT GCCAGGGCTG
    CCTGGTGAGA AGGGCCAAAG GGGTGACTTT GGTTATGTGG GGAAACCTGG TCCCCCAGGA
    GAGGATGGAC GGAAGGGAGC AGAGGGACCT CCGGGGCCCA CTGGCCAGGC AGGGGAGCCG
    GGCCCCCGAG GACTGATTGG CCCTAGAGGC TCCCCCGGAC CCCTGGGACG CCCGGGTGTG
    ATTGGAAGTG ATGGTGCTCC AGGTGCCAAA GGAAATGTGG GTCCTCCAGG AGAACCAGGC
    CCCCCAGGAC AGCAGGGAAA TCCTGGGTCC CAGGGAATTC CCGGCCCCCA GGGACCCATC
    GGCACTCCTG GGGAGAAGGG TCCTCCTGGA AACCCAGGAA TTCCAGGCCT CCCAGGATCT
    GAAGGCCCTC CGGGTCACCC AGGTCATGAG GGCCCAACAG GAGAGAAAGG GGCCCAGGGT
    CCAGCAGGGT CAGCAGGCCC TCCGGGCTAT CCAGGACCTC GGGGTGTGAA GGGTACCTCT
    GGCAACCGGG GCCTCCAGGG GGAGAAAGGA GAGAAGGGAG AGGATGGTTT CCCAGGCTTC
    AAGGGTGATG CGGGGCTTAA AGGAGATCGG GGGCAACCCG GACCTCCAGG TCCTCGGGGA
    GAGGATGGTC CGGAGGGGCT AAAGGGGCAG GAGGGGCTGC TTGGCGAGGA GGGTCCCCCA
    GGCTCAGCTG GGGAGAAGGG CAAGCTTGGC GTGCCGGGTC TCCCAGGTTA TCCAGGACGT
    CCAGGCCCTA AGGGATCTAT TGGCTTTCCG GGACCTCTGG GACCATTAGG AGAAAAAGGG
    AAGCGGGGGA AGGCTGGGCA GCCAGGCCTG GAAGGAGAGC GGGGACCACC AGGTGCCCGT
    GGAGAGAGAG GGCAACCAGG TGCCACAGGG CAGCCGGGCC CCAAGGGTGA TGTGGGCCAG
    GACGGAGCCC CTGGGTTCCC TGGAGAAAAG GGCCTCCCAG GTCTGCAAGG TCCTCCTGGG
    TTCTCTGGAC CAAAGGGTCC CCCTGGTCCC CAGGGAAAAG ATGGGGTACC TGGACACCCT
    GGACAGAGAG GAGAATTGGG TTTCCAAGGT CAGACAGGCC CACCTGGACC AGCTGGTGTC
    GTGGGTCCTC AGGGAAAGAC AGGAGAAGCA GGGCCTCTGG GTGAGAGGGG GCCCCCAGGG
    CCCCCTGGAC CTCCTGGTGA ACAAGGTCTT CCGGGCCTGG AAGGCAGAGA GGGGGCCAAG
    GGGGATGTGG GACCACTGGG ACCCCTTGGG AAGGAAGGGC CACCTGGACC CAGAGGTTTC
    CCTGGTCCTC ATGGAGCCCC CGGGGACCCA GGACCGACTG GTTTAAAGGG TGACAAGGGC
    CCCCCAGGTC CTGTTGGTGC CAACGGATCC CCTGGGGAGC GTGGTCCTGT AGGCCCAGCA
    GGGAGCATTG GGCTTCCTGG TCAAAGCGGA GGCCAAGGCC CCGTGGGCCC CACAGGCGAG
    AAGGGTTCCC CGGGTGAACG TGGCGCCCCT GGCCCAACTG GTAAAGATGG GATCCCCGGG
    CCCCTGGGGC TTCCTGGGCC CCCTGGAGCT GCGGGGCCTT CTGGCGAGGA AGGGGACAAG
    GGAGAAGTGG GTGCCCCCGG TCACAAAGGC AGCAAAGGTG ACAAGGGAGA TGCGGGCCCG
    CCTGGACCAA CAGGGATACG GGGTCTGGTG GGACACCCCG GCTCCCCGGG AGCAGACGGG
    GCTCAGGGAC GCCGGGGACC CCCAGGCCTC TTTGGGCAGA AAGGAGATGA TGGAGTGAGA
    GGCTTCCCTG GCATGATTGG TCCTCCTGGC CTACAGGGAA TGCCAGGCCC TGCTGGAGAA
    AAGGGAGAAG TTGGAGATGT AGGGTCCATG GGTCCCCACG GAGCTCCAGG CCCTCGGGGC
    CCCCAGGGCC CCAGCGGATC AGAGGGTCCT CCAGGGTTGC CTGGGGGAGT TGGTCAGCCT
    GGCGCTGTGG GGGAGAAGGG TGAGCCAGGA GAGCCTGGAG ACCCAGGACC CCCCGGGACC
    CCAGGCACCC CGGGCCCCAG GGGAGAAGTT GGTGAAAAGG GGGATACAGG CCCATCTGGG
    GCTGCTGGAC CCCCAGGCAA GAAAGGACCC CCTGGAGAGG ATGGAGCAAA GGGGAATGTG
    GGCCCCACAG GCCTCCCAGG AGACCTAGGC CCCCCCGGAG ACCCTGGAGT TTCGGGTCAA
    GATGGCCCCC CAGGGGAGAA GGGAAACCCT GGAGATGTGG GGGGACCGGG TCCACCTGGC
    GCTTCTGGGG AGCCTGGCGC CCCTGGGCCT CCTGGTAAAA GGGGTCCTTC TGGTCGCATG
    GGTCAAGAAG GTAGAGAAGG AGAGAAAGGT GCCAAGGGGG AACCAGGTCC TGATGGACCC
    CCAGGGAGGA CAGGCCCAGT AGGGGCTCGA GGGCCCCCTG GACGTGTTGG ACCTGAGGGT
    CTTCGTGGGA TCCCTGGACC TGTGGGCGAA CCAGGCCTCC TGGGACCTCC TGGACTGTTA
    GGCCCTCCTG GTCCCCTGGG GCCCTCTGGC CTACCAGGGC TGAAGGGAGA TGCTGGCCCC
    AAGGGAGAAA AGGGCCACAT TGGATTAATT GGTCTTGTCG GCCCCCCTGG GGAGGTTGGT
    GAGAAAGGGG ATCAGGGGTT GCCAGGAGTG CAAGGCTCCC CTGGCCTCCA GGGAGAACCG
    GGTTCTCCTG GCCCCATTGG CTCTCTGGGC CACCCTGGGC CCCAAGGCGT GGTGGGTCCT
    CCAGGACAGA AAGGCTCAAA GGGGTCCCCG GGATCCCAAG GTCCTCGTGG AGACGCTGGC
    CCCCCAGGTC CTCCTGGTCC CCCGGGTCCC TCTGCGGAGC TGCACGGGCT GCGCCGGCGC
    CGACGTTCCC TGCTAGGCTT GGGAACCCCG GAGGGCGGCC TGGAGGAGGT GCTGGCCTCG
    ATGGCGTCGC TGAGCTTCGA GTTAGAGCAG CTGCGGCGCC CTCCCGGCAC GGCTGAGCGC
    CCGGGCCTCG TGTGCAAAGA GTTGCACCGC AGCCATCCGC ATCTGCCAGA TGGGGAATAC
    TGGATTGATC CCAATCAAGG CTGCGCGCGG GATGCCCTCA GGGTTTTCTG TAACTTCACG
    GCAGGAGGAG AAACCTGCCT CTACCCAGAC AAGAAGTTTG AGACCGTGAA ACTGGCCTCT
    TGGTCCAAAG AAAAGCCAGG TGGCTGGTAT AGCACATTCC GTCGAGGGAA AAAGTTCTCC
    TACGTGGATG CCGATGGGGC CGCAGTGAGC GTGGTACAGC TGACCTTCCT GAAGTTGCTG
    AGTGCCACGG CCCGCCAGAG CTTCACATTC ACCTGCCAGA ACTCGGCCGC CTGGCTGGAC
    GAAGCTGCAG GTGACCACAG CCGCTCTGCC CGTTTCCTCG GGGCCAATGG GGAGGAGGTA
    TCCTTCAACC AGATGGCAGC CGCCACCATC ACAGTCCCCT ACGATGGCTG CAGGCTCCGG
    AAGGGACAGT CGAAGACCCT CTTTGAGTTC AGCTCCTCCC GAGTGGGATT TCTGCCCCTG
    TTGGACGTGG CAGCTGCTGA CTTTGGCCAG ACGAACCAGA AGTTTGGGTT TGAACTGGGC
    CCTGTCTGCT TTAGCAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235872
    Clone ID Related Accession (Same CDS sequence) XM_015472002.2
    Accession Version XM_015472002.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5220bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-3(V) chain isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472002.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCCGGAC GCAGGCCGTA GATCCTGTGG ATGTGCTGAA GGCACTGGGC
    ATGCGGGGGG GCCAGGCTGG GATCCCTGAG GGACCCGGCC TCTGCCCCCA GAGGGTCCCG
    GAGGGCGACC GAGCATTCCG GGTGGGCAAG ACTGGCACAC TTGGTGTCCC CACGCAGGAG
    CTCTTCCAAG ATGGGCACTT TCCTGAGAAC TTCTCCATCC TGATCACCCT GCGGGGCCAG
    CCGGCCAACC GTTCTGTCCT TCTGTCCATC TATGATGAGG GCGGCGTCCG GCAGCTCGGC
    TTGGCCTTGG GGCCAGCTCT GGACCTCCTA GGTGACTCCT TCAGCCCCCT CCCCCAGCAG
    GTCAACCTCA CGGATGGCAG GTGGCACCGT GTGGCAGTCA GTGTGGACGG TGGGATGGTG
    ACCCTGGTGG CTGATTGTGA ACCTCAGCTC CCCATGTTGG CTCAGGGACC TCGATTCATC
    AGTACAGCGG GACTCACTGT GCTGGGGACC CAGGACCTTG GCGAGGAGAC TTTTGAGGGA
    GATGTTCAGG AGCTGGTGAT AAGCCCAGAT CCCCAGGCTG CCTTCCAGGC ATGTGAGCGG
    TACCTTCCTG GCTGTGATGA CCTGGATCCA ACAGTCACCA CGGTCCCTCA GGGGGAGCCA
    GAAACCCCTC CCCCTAGGCG GAAGGGGAAA GGAAAAGGGA AGAAAAAAGG GCGAGGTCGT
    AAAGGGAAGG GCAGGAAGAA GAAGAACAAG GAAACTGTGG CTGTGAGTCC TCCTCCTGGC
    TCCCTGGACA ACCAGACCTC CACTGACATC CCCAAGACAG AGACACCAGA ACCAACTCTG
    TCTCCGACAC CCACGCCTTT GGTCATCACC ACAACTGTGA CCTTTGGTAG CAATGTCACC
    ATCCTAGAGG AGGGCCTGGA CCCTGACAAT GGAACTGAAC TGGAGACCCT GGAGACTGAG
    TTAGACAGAG AAGATGAAGA AGAAGGTGAT CCCACCATGG GCCCCCACTT CCGGGCAGCA
    GAACAGTCAT GGAATACTGA GTTCCAGATC TTTCCTGGTG CTGGGGAGAA AGGAGAAAAA
    GGAGAACCAG CTGTGGTCGA ACAGGGACAG CAGTTTGAAG GACCCCCAGG AGCCCCGGGA
    CCCCAAGGGG TGGTTGGCCC CTCAGGCCCT CCTGGCCCCC CAGGATTCCC TGGAGACCGT
    GGTCTACCGG GCCCTGCTGG CCTCCCAGGC ATCCCTGGCA TGGATGGGAT CCGGGGTCTC
    CCAGGCACTG TGATCATGAT GCCGTTCCAG TTTGCAAGAG GCTCTCACAA AGGACCCCCA
    GTATCCTTCC AGCAGGCCCA GGCTCAGGCA GTCCTGCAAC AAGCTCAGCT GTCTATGAAA
    GGCCCTCCTG GCCCGGTGGG GCTCACCGGA CGCCCAGGTC CCGTGGGTCT TCCTGGGTAT
    CCAGGTCTGA AAGGAGAGAT GGGAGAAATG GGGCCGCAGG GTCCCCGAGG GCTTCAGGGA
    CCTCATGGGC CCCCTGGCCG AGAGGGAAAG ATGGGCCGCG CTGGAGCAGA TGGGGCTCGG
    GGCCTCCCCG GCGCTACGGG ACCTAAGGGT GATCGGGGCT TTGATGGCCT GCCAGGGCTG
    CCTGGTGAGA AGGGCCAAAG GGGTGACTTT GGTTATGTGG GGAAACCTGG TCCCCCAGGA
    GAGGATGGAC GGAAGGGAGC AGAGGGACCT CCGGGGCCCA CTGGCCAGGC AGGGGAGCCG
    GGCCCCCGAG GACTGATTGG CCCTAGAGGC TCCCCCGGAC CCCTGGGACG CCCGGGTGTG
    ATTGGAAGTG ATGGTGCTCC AGGTGCCAAA GGAAATGTGG GTCCTCCAGG AGAACCAGGC
    CCCCCAGGAC AGCAGGGAAA TCCTGGGTCC CAGGGAATTC CCGGCCCCCA GGGACCCATC
    GGCACTCCTG GGGAGAAGGG TCCTCCTGGA AACCCAGGAA TTCCAGGCCT CCCAGGATCT
    GAAGGCCCTC CGGGTCACCC AGGTCATGAG GGCCCAACAG GAGAGAAAGG GGCCCAGGGT
    CCAGCAGGGT CAGCAGGCCC TCCGGGCTAT CCAGGACCTC GGGGTGTGAA GGGTACCTCT
    GGCAACCGGG GCCTCCAGGG GGAGAAAGGA GAGAAGGGAG AGGATGGTTT CCCAGGCTTC
    AAGGGTGATG CGGGGCTTAA AGGAGATCGG GGGCAACCCG GACCTCCAGG TCCTCGGGGA
    GAGGATGGTC CGGAGGGGCT AAAGGGGCAG GAGGGGCTGC TTGGCGAGGA GGGTCCCCCA
    GGCTCAGCTG GGGAGAAGGG CAAGCTTGGC GTGCCGGGTC TCCCAGGTTA TCCAGGACGT
    CCAGGCCCTA AGGGATCTAT TGGCTTTCCG GGACCTCTGG GACCATTAGG AGAAAAAGGG
    AAGCGGGGGA AGGCTGGGCA GCCAGGCCTG GAAGGAGAGC GGGGACCACC AGGTGCCCGT
    GGAGAGAGAG GGCAACCAGG TGCCACAGGG CAGCCGGGCC CCAAGGGTGA TGTGGGCCAG
    GACGGAGCCC CTGGGTTCCC TGGAGAAAAG GGCCTCCCAG GTCTGCAAGG TCCTCCTGGG
    TTCTCTGGAC CAAAGGGTCC CCCTGGTCCC CAGGGAAAAG ATGGGGTACC TGGACACCCT
    GGACAGAGAG GAGAATTGGG TTTCCAAGGT CAGACAGGCC CACCTGGACC AGCTGGTGTC
    GTGGGTCCTC AGGGAAAGAC AGGAGAAGCA GGGCCTCTGG GTGAGAGGGG GCCCCCAGGG
    CCCCCTGGAC CTCCTGGTGA ACAAGGTCTT CCGGGCCTGG AAGGCAGAGA GGGGGCCAAG
    GGGGATGTGG GACCACTGGG ACCCCTTGGG AAGGAAGGGC CACCTGGACC CAGAGGTTTC
    CCTGGTCCTC ATGGAGCCCC CGGGGACCCA GGACCGACTG GTTTAAAGGG TGACAAGGGC
    CCCCCAGGTC CTGTTGGTGC CAACGGATCC CCTGGGGAGC GTGGTCCTGT AGGCCCAGCA
    GGGAGCATTG GGCTTCCTGG TCAAAGCGGA GGCCAAGGCC CCGTGGGCCC CACAGGCGAG
    AAGGGTTCCC CGGGTGAACG TGGCGCCCCT GGCCCAACTG GTAAAGATGG GATCCCCGGG
    CCCCTGGGGC TTCCTGGGCC CCCTGGAGCT GCGGGGCCTT CTGGCGAGGA AGGGGACAAG
    GGAGAAGTGG GTGCCCCCGG TCACAAAGGC AGCAAAGGTG ACAAGGGAGA TGCGGGCCCG
    CCTGGACCAA CAGGGATACG GGGTCTGGTG GGACACCCCG GCTCCCCGGG AGCAGACGGG
    GCTCAGGGAC GCCGGGGACC CCCAGGCCTC TTTGGGCAGA AAGGAGATGA TGGAGTGAGA
    GGCTTCCCTG GCATGATTGG TCCTCCTGGC CTACAGGGAA TGCCAGGCCC TGCTGGAGAA
    AAGGGAGAAG TTGGAGATGT AGGGTCCATG GGTCCCCACG GAGCTCCAGG CCCTCGGGGC
    CCCCAGGGCC CCAGCGGATC AGAGGGTCCT CCAGGGTTGC CTGGGGGAGT TGGTCAGCCT
    GGCGCTGTGG GGGAGAAGGG TGAGCCAGGA GAGCCTGGAG ACCCAGGACC CCCCGGGACC
    CCAGGCACCC CGGGCCCCAG GGGAGAAGTT GGTGAAAAGG GGGATACAGG CCCATCTGGG
    GCTGCTGGAC CCCCAGGCAA GAAAGGACCC CCTGGAGAGG ATGGAGCAAA GGGGAATGTG
    GGCCCCACAG GCCTCCCAGG AGACCTAGGC CCCCCCGGAG ACCCTGGAGT TTCGGGTCAA
    GATGGCCCCC CAGGGGAGAA GGGAAACCCT GGAGATGTGG GGGGACCGGG TCCACCTGGC
    GCTTCTGGGG AGCCTGGCGC CCCTGGGCCT CCTGGTAAAA GGGGTCCTTC TGGTCGCATG
    GGTCAAGAAG GTAGAGAAGG AGAGAAAGGT GCCAAGGGGG AACCAGGTCC TGATGGACCC
    CCAGGGAGGA CAGGCCCAGT AGGGGCTCGA GGGCCCCCTG GACGTGTTGG ACCTGAGGGT
    CTTCGTGGGA TCCCTGGACC TGTGGGCGAA CCAGGCCTCC TGGGACCTCC TGGACTGTTA
    GGCCCTCCTG GTCCCCTGGG GCCCTCTGGC CTACCAGGGC TGAAGGGAGA TGCTGGCCCC
    AAGGGAGAAA AGGGCCACAT TGGATTAATT GGTCTTGTCG GCCCCCCTGG GGAGGTTGGT
    GAGAAAGGGG ATCAGGGGTT GCCAGGAGTG CAAGGCTCCC CTGGCCTCCA GGGAGAACCG
    GGTTCTCCTG GCCCCATTGG CTCTCTGGGC CACCCTGGGC CCCAAGGCGT GGTGGGTCCT
    CCAGGACAGA AAGGCTCAAA GGGGTCCCCG GGATCCCAAG GTCCTCGTGG AGACGCTGGC
    CCCCCAGGTC CTCCTGGTCC CCCGGGTCCC TCTGCGGAGC TGCACGGGCT GCGCCGGCGC
    CGACGTTCCC TGCTAGGCTT GGGAACCCCG GAGGGCGGCC TGGAGGAGGT GCTGGCCTCG
    ATGGCGTCGC TGAGCTTCGA GTTAGAGCAG CTGCGGCGCC CTCCCGGCAC GGCTGAGCGC
    CCGGGCCTCG TGTGCAAAGA GTTGCACCGC AGCCATCCGC ATCTGCCAGA TGGGGAATAC
    TGGATTGATC CCAATCAAGG CTGCGCGCGG GATGCCCTCA GGGTTTTCTG TAACTTCACG
    GCAGGAGGAG AAACCTGCCT CTACCCAGAC AAGAAGTTTG AGACCGTGAA ACTGGCCTCT
    TGGTCCAAAG AAAAGCCAGG TGGCTGGTAT AGCACATTCC GTCGAGGGAA AAAGTTCTCC
    TACGTGGATG CCGATGGGGC CGCAGTGAGC GTGGTACAGC TGACCTTCCT GAAGTTGCTG
    AGTGCCACGG CCCGCCAGAG CTTCACATTC ACCTGCCAGA ACTCGGCCGC CTGGCTGGAC
    GAAGCTGCAG GTGGGTTCGC AGTGAAGGGA CTGATGTCAG ACTACAGAGG GAAGTGGGAC
    TTCCCTGGAC TGAGCTGCGT ACTCTTTCTG CTGCCTCAGC CCAGCTCTGA ATACTGTCTC
    CCTCCATGCC TCCCCAAATA CACCTTGCCA TGCCAGGTGG CGTGGGGACC AAACTGGTGG
    GGTTGGGGGG AAACTAGGAT TCTAATGGTG CTGCCCTATT TATACCTGGG TCTATATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327488.2
    CDS157..5376
    Translation

    Target ORF information:

    RefSeq Version XM_015472002.2
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type V alpha 3 chain (COL5A3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472002.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCCGGAC GCAGGCCGTA GATCCTGTGG ATGTGCTGAA GGCACTGGGC
    ATGCGGGGGG GCCAGGCTGG GATCCCTGAG GGACCCGGCC TCTGCCCCCA GAGGGTCCCG
    GAGGGCGACC GAGCATTCCG GGTGGGCAAG ACTGGCACAC TTGGTGTCCC CACGCAGGAG
    CTCTTCCAAG ATGGGCACTT TCCTGAGAAC TTCTCCATCC TGATCACCCT GCGGGGCCAG
    CCGGCCAACC GTTCTGTCCT TCTGTCCATC TATGATGAGG GCGGCGTCCG GCAGCTCGGC
    TTGGCCTTGG GGCCAGCTCT GGACCTCCTA GGTGACTCCT TCAGCCCCCT CCCCCAGCAG
    GTCAACCTCA CGGATGGCAG GTGGCACCGT GTGGCAGTCA GTGTGGACGG TGGGATGGTG
    ACCCTGGTGG CTGATTGTGA ACCTCAGCTC CCCATGTTGG CTCAGGGACC TCGATTCATC
    AGTACAGCGG GACTCACTGT GCTGGGGACC CAGGACCTTG GCGAGGAGAC TTTTGAGGGA
    GATGTTCAGG AGCTGGTGAT AAGCCCAGAT CCCCAGGCTG CCTTCCAGGC ATGTGAGCGG
    TACCTTCCTG GCTGTGATGA CCTGGATCCA ACAGTCACCA CGGTCCCTCA GGGGGAGCCA
    GAAACCCCTC CCCCTAGGCG GAAGGGGAAA GGAAAAGGGA AGAAAAAAGG GCGAGGTCGT
    AAAGGGAAGG GCAGGAAGAA GAAGAACAAG GAAACTGTGG CTGTGAGTCC TCCTCCTGGC
    TCCCTGGACA ACCAGACCTC CACTGACATC CCCAAGACAG AGACACCAGA ACCAACTCTG
    TCTCCGACAC CCACGCCTTT GGTCATCACC ACAACTGTGA CCTTTGGTAG CAATGTCACC
    ATCCTAGAGG AGGGCCTGGA CCCTGACAAT GGAACTGAAC TGGAGACCCT GGAGACTGAG
    TTAGACAGAG AAGATGAAGA AGAAGGTGAT CCCACCATGG GCCCCCACTT CCGGGCAGCA
    GAACAGTCAT GGAATACTGA GTTCCAGATC TTTCCTGGTG CTGGGGAGAA AGGAGAAAAA
    GGAGAACCAG CTGTGGTCGA ACAGGGACAG CAGTTTGAAG GACCCCCAGG AGCCCCGGGA
    CCCCAAGGGG TGGTTGGCCC CTCAGGCCCT CCTGGCCCCC CAGGATTCCC TGGAGACCGT
    GGTCTACCGG GCCCTGCTGG CCTCCCAGGC ATCCCTGGCA TGGATGGGAT CCGGGGTCTC
    CCAGGCACTG TGATCATGAT GCCGTTCCAG TTTGCAAGAG GCTCTCACAA AGGACCCCCA
    GTATCCTTCC AGCAGGCCCA GGCTCAGGCA GTCCTGCAAC AAGCTCAGCT GTCTATGAAA
    GGCCCTCCTG GCCCGGTGGG GCTCACCGGA CGCCCAGGTC CCGTGGGTCT TCCTGGGTAT
    CCAGGTCTGA AAGGAGAGAT GGGAGAAATG GGGCCGCAGG GTCCCCGAGG GCTTCAGGGA
    CCTCATGGGC CCCCTGGCCG AGAGGGAAAG ATGGGCCGCG CTGGAGCAGA TGGGGCTCGG
    GGCCTCCCCG GCGCTACGGG ACCTAAGGGT GATCGGGGCT TTGATGGCCT GCCAGGGCTG
    CCTGGTGAGA AGGGCCAAAG GGGTGACTTT GGTTATGTGG GGAAACCTGG TCCCCCAGGA
    GAGGATGGAC GGAAGGGAGC AGAGGGACCT CCGGGGCCCA CTGGCCAGGC AGGGGAGCCG
    GGCCCCCGAG GACTGATTGG CCCTAGAGGC TCCCCCGGAC CCCTGGGACG CCCGGGTGTG
    ATTGGAAGTG ATGGTGCTCC AGGTGCCAAA GGAAATGTGG GTCCTCCAGG AGAACCAGGC
    CCCCCAGGAC AGCAGGGAAA TCCTGGGTCC CAGGGAATTC CCGGCCCCCA GGGACCCATC
    GGCACTCCTG GGGAGAAGGG TCCTCCTGGA AACCCAGGAA TTCCAGGCCT CCCAGGATCT
    GAAGGCCCTC CGGGTCACCC AGGTCATGAG GGCCCAACAG GAGAGAAAGG GGCCCAGGGT
    CCAGCAGGGT CAGCAGGCCC TCCGGGCTAT CCAGGACCTC GGGGTGTGAA GGGTACCTCT
    GGCAACCGGG GCCTCCAGGG GGAGAAAGGA GAGAAGGGAG AGGATGGTTT CCCAGGCTTC
    AAGGGTGATG CGGGGCTTAA AGGAGATCGG GGGCAACCCG GACCTCCAGG TCCTCGGGGA
    GAGGATGGTC CGGAGGGGCT AAAGGGGCAG GAGGGGCTGC TTGGCGAGGA GGGTCCCCCA
    GGCTCAGCTG GGGAGAAGGG CAAGCTTGGC GTGCCGGGTC TCCCAGGTTA TCCAGGACGT
    CCAGGCCCTA AGGGATCTAT TGGCTTTCCG GGACCTCTGG GACCATTAGG AGAAAAAGGG
    AAGCGGGGGA AGGCTGGGCA GCCAGGCCTG GAAGGAGAGC GGGGACCACC AGGTGCCCGT
    GGAGAGAGAG GGCAACCAGG TGCCACAGGG CAGCCGGGCC CCAAGGGTGA TGTGGGCCAG
    GACGGAGCCC CTGGGTTCCC TGGAGAAAAG GGCCTCCCAG GTCTGCAAGG TCCTCCTGGG
    TTCTCTGGAC CAAAGGGTCC CCCTGGTCCC CAGGGAAAAG ATGGGGTACC TGGACACCCT
    GGACAGAGAG GAGAATTGGG TTTCCAAGGT CAGACAGGCC CACCTGGACC AGCTGGTGTC
    GTGGGTCCTC AGGGAAAGAC AGGAGAAGCA GGGCCTCTGG GTGAGAGGGG GCCCCCAGGG
    CCCCCTGGAC CTCCTGGTGA ACAAGGTCTT CCGGGCCTGG AAGGCAGAGA GGGGGCCAAG
    GGGGATGTGG GACCACTGGG ACCCCTTGGG AAGGAAGGGC CACCTGGACC CAGAGGTTTC
    CCTGGTCCTC ATGGAGCCCC CGGGGACCCA GGACCGACTG GTTTAAAGGG TGACAAGGGC
    CCCCCAGGTC CTGTTGGTGC CAACGGATCC CCTGGGGAGC GTGGTCCTGT AGGCCCAGCA
    GGGAGCATTG GGCTTCCTGG TCAAAGCGGA GGCCAAGGCC CCGTGGGCCC CACAGGCGAG
    AAGGGTTCCC CGGGTGAACG TGGCGCCCCT GGCCCAACTG GTAAAGATGG GATCCCCGGG
    CCCCTGGGGC TTCCTGGGCC CCCTGGAGCT GCGGGGCCTT CTGGCGAGGA AGGGGACAAG
    GGAGAAGTGG GTGCCCCCGG TCACAAAGGC AGCAAAGGTG ACAAGGGAGA TGCGGGCCCG
    CCTGGACCAA CAGGGATACG GGGTCTGGTG GGACACCCCG GCTCCCCGGG AGCAGACGGG
    GCTCAGGGAC GCCGGGGACC CCCAGGCCTC TTTGGGCAGA AAGGAGATGA TGGAGTGAGA
    GGCTTCCCTG GCATGATTGG TCCTCCTGGC CTACAGGGAA TGCCAGGCCC TGCTGGAGAA
    AAGGGAGAAG TTGGAGATGT AGGGTCCATG GGTCCCCACG GAGCTCCAGG CCCTCGGGGC
    CCCCAGGGCC CCAGCGGATC AGAGGGTCCT CCAGGGTTGC CTGGGGGAGT TGGTCAGCCT
    GGCGCTGTGG GGGAGAAGGG TGAGCCAGGA GAGCCTGGAG ACCCAGGACC CCCCGGGACC
    CCAGGCACCC CGGGCCCCAG GGGAGAAGTT GGTGAAAAGG GGGATACAGG CCCATCTGGG
    GCTGCTGGAC CCCCAGGCAA GAAAGGACCC CCTGGAGAGG ATGGAGCAAA GGGGAATGTG
    GGCCCCACAG GCCTCCCAGG AGACCTAGGC CCCCCCGGAG ACCCTGGAGT TTCGGGTCAA
    GATGGCCCCC CAGGGGAGAA GGGAAACCCT GGAGATGTGG GGGGACCGGG TCCACCTGGC
    GCTTCTGGGG AGCCTGGCGC CCCTGGGCCT CCTGGTAAAA GGGGTCCTTC TGGTCGCATG
    GGTCAAGAAG GTAGAGAAGG AGAGAAAGGT GCCAAGGGGG AACCAGGTCC TGATGGACCC
    CCAGGGAGGA CAGGCCCAGT AGGGGCTCGA GGGCCCCCTG GACGTGTTGG ACCTGAGGGT
    CTTCGTGGGA TCCCTGGACC TGTGGGCGAA CCAGGCCTCC TGGGACCTCC TGGACTGTTA
    GGCCCTCCTG GTCCCCTGGG GCCCTCTGGC CTACCAGGGC TGAAGGGAGA TGCTGGCCCC
    AAGGGAGAAA AGGGCCACAT TGGATTAATT GGTCTTGTCG GCCCCCCTGG GGAGGTTGGT
    GAGAAAGGGG ATCAGGGGTT GCCAGGAGTG CAAGGCTCCC CTGGCCTCCA GGGAGAACCG
    GGTTCTCCTG GCCCCATTGG CTCTCTGGGC CACCCTGGGC CCCAAGGCGT GGTGGGTCCT
    CCAGGACAGA AAGGCTCAAA GGGGTCCCCG GGATCCCAAG GTCCTCGTGG AGACGCTGGC
    CCCCCAGGTC CTCCTGGTCC CCCGGGTCCC TCTGCGGAGC TGCACGGGCT GCGCCGGCGC
    CGACGTTCCC TGCTAGGCTT GGGAACCCCG GAGGGCGGCC TGGAGGAGGT GCTGGCCTCG
    ATGGCGTCGC TGAGCTTCGA GTTAGAGCAG CTGCGGCGCC CTCCCGGCAC GGCTGAGCGC
    CCGGGCCTCG TGTGCAAAGA GTTGCACCGC AGCCATCCGC ATCTGCCAGA TGGGGAATAC
    TGGATTGATC CCAATCAAGG CTGCGCGCGG GATGCCCTCA GGGTTTTCTG TAACTTCACG
    GCAGGAGGAG AAACCTGCCT CTACCCAGAC AAGAAGTTTG AGACCGTGAA ACTGGCCTCT
    TGGTCCAAAG AAAAGCCAGG TGGCTGGTAT AGCACATTCC GTCGAGGGAA AAAGTTCTCC
    TACGTGGATG CCGATGGGGC CGCAGTGAGC GTGGTACAGC TGACCTTCCT GAAGTTGCTG
    AGTGCCACGG CCCGCCAGAG CTTCACATTC ACCTGCCAGA ACTCGGCCGC CTGGCTGGAC
    GAAGCTGCAG GTGGGTTCGC AGTGAAGGGA CTGATGTCAG ACTACAGAGG GAAGTGGGAC
    TTCCCTGGAC TGAGCTGCGT ACTCTTTCTG CTGCCTCAGC CCAGCTCTGA ATACTGTCTC
    CCTCCATGCC TCCCCAAATA CACCTTGCCA TGCCAGGTGG CGTGGGGACC AAACTGGTGG
    GGTTGGGGGG AAACTAGGAT TCTAATGGTG CTGCCCTATT TATACCTGGG TCTATATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235873
    Clone ID Related Accession (Same CDS sequence) XM_024993999.1
    Accession Version XM_024993999.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5022bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-3(V) chain isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCCGGAC GCAGGCCGTA GATCCTGTGG ATGTGCTGAA GGCACTGGGC
    ATGCGGGGGG GCCAGGCTGG GATCCCTGAG GGACCCGGCC TCTGCCCCCA GAGGGTCCCG
    GAGGGCGACC GAGCATTCCG GGTGGGCAAG ACTGGCACAC TTGGTGTCCC CACGCAGGAG
    CTCTTCCAAG ATGGGCACTT TCCTGAGAAC TTCTCCATCC TGATCACCCT GCGGGGCCAG
    CCGGCCAACC GTTCTGTCCT TCTGTCCATC TATGATGAGG GCGGCGTCCG GCAGCTCGGC
    TTGGCCTTGG GGCCAGCTCT GGACCTCCTA GGTGACTCCT TCAGCCCCCT CCCCCAGCAG
    GTCAACCTCA CGGATGGCAG GTGGCACCGT GTGGCAGTCA GTGTGGACGG TGGGATGGTG
    ACCCTGGTGG CTGATTGTGA ACCTCAGCTC CCCATGTTGG CTCAGGGACC TCGATTCATC
    AGTACAGCGG GACTCACTGT GCTGGGGACC CAGGACCTTG GCGAGGAGAC TTTTGAGGGA
    GATGTTCAGG AGCTGGTGAT AAGCCCAGAT CCCCAGGCTG CCTTCCAGGC ATGTGAGCGG
    TACCTTCCTG GCTGTGATGA CCTGGATCCA ACAGTCACCA CGGTCCCTCA GGGGGAGCCA
    GAAACCCCTC CCCCTAGGCG GAAGGGGAAA GGAAAAGGGA AGAAAAAAGG GCGAGGTCGT
    AAAGGGAAGG GCAGGAAGAA GAAGAACAAG GAAACTGTGG CTGTGAGTCC TCCTCCTGGC
    TCCCTGGACA ACCAGACCTC CACTGACATC CCCAAGACAG AGACACCAGA ACCAACTCTG
    TCTCCGACAC CCACGCCTTT GGTCATCACC ACAACTGTGA CCTTTGGTAG CAATGTCACC
    ATCCTAGAGG AGGGCCTGGA CCCTGACAAT GGAACTGAAC TGGAGACCCT GGAGACTGAG
    TTAGACAGAG AAGATGAAGA AGAAGGTGAT CCCACCATGG GCCCCCACTT CCGGGCAGCA
    GAACAGTCAT GGAATACTGA GTTCCAGATC TTTCCTGGTG CTGGGGAGAA AGGAGAAAAA
    GGAGAACCAG CTGTGGTCGA ACAGGGACAG CAGTTTGAAG GACCCCCAGG AGCCCCGGGA
    CCCCAAGGGG TGGTTGGCCC CTCAGGCCCT CCTGGCCCCC CAGGATTCCC TGGAGACCGT
    GGTCTACCGG GCCCTGCTGG CCTCCCAGGC ATCCCTGGCA TGGATGGGAT CCGGGGTCTC
    CCAGGCACTG TGATCATGAT GCCGTTCCAG TTTGCAAGAG GCTCTCACAA AGGACCCCCA
    GTATCCTTCC AGCAGGCCCA GGCTCAGGCA GTCCTGCAAC AAGCTCAGCT GTCTATGAAA
    GGCCCTCCTG GCCCGGTGGG GCTCACCGGA CGCCCAGGTC CCGTGGGTCT TCCTGGGTAT
    CCAGGTCTGA AAGGAGAGAT GGGAGAAATG GGGCCGCAGG GTCCCCGAGG GCTTCAGGGA
    CCTCATGGGC CCCCTGGCCG AGAGGGAAAG ATGGGCCGCG CTGGAGCAGA TGGGGCTCGG
    GGCCTCCCCG GCGCTACGGG ACCTAAGGGT GATCGGGGCT TTGATGGCCT GCCAGGGCTG
    CCTGGTGAGA AGGGCCAAAG GGGTGACTTT GGTTATGTGG GGAAACCTGG TCCCCCAGGA
    GAGGATGGAC GGAAGGGAGC AGAGGGACCT CCGGGGCCCA CTGGCCAGGC AGGGGAGCCG
    GGCCCCCGAG GACTGATTGG CCCTAGAGGC TCCCCCGGAC CCCTGGGACG CCCGGGTGTG
    ATTGGAAGTG ATGGTGCTCC AGGTGCCAAA GGAAATGTGG GTCCTCCAGG AGAACCAGGC
    CCCCCAGGAC AGCAGGGAAA TCCTGGGTCC CAGGGAATTC CCGGCCCCCA GGGACCCATC
    GGCACTCCTG GGGAGAAGGG TCCTCCTGGA AACCCAGGAA TTCCAGGCCT CCCAGGATCT
    GAAGGCCCTC CGGGTCACCC AGGTCATGAG GGCCCAACAG GAGAGAAAGG GGCCCAGGGT
    CCAGCAGGGT CAGCAGGCCC TCCGGGCTAT CCAGGACCTC GGGGTGTGAA GGGTACCTCT
    GGCAACCGGG GCCTCCAGGG GGAGAAAGGA GAGAAGGGAG AGGATGGTTT CCCAGGCTTC
    AAGGGTGATG CGGGGCTTAA AGGAGATCGG GGGCAACCCG GACCTCCAGG TCCTCGGGGA
    GAGGATGGTC CGGAGGGGCT AAAGGGGCAG GAGGGGCTGC TTGGCGAGGA GGGTCCCCCA
    GGCTCAGCTG GGGAGAAGGG CAAGCTTGGC GTGCCGGGTC TCCCAGGTTA TCCAGGACGT
    CCAGGCCCTA AGGGATCTAT TGGCTTTCCG GGACCTCTGG GACCATTAGG AGAAAAAGGG
    AAGCGGGGGA AGGCTGGGCA GCCAGGCCTG GAAGGAGAGC GGGGACCACC AGGTGCCCGT
    GGAGAGAGAG GGCAACCAGG TGCCACAGGG CAGCCGGGCC CCAAGGGTGA TGTGGGCCAG
    GACGGAGCCC CTGGGTTCCC TGGAGAAAAG GGCCTCCCAG GTCTGCAAGG TCCTCCTGGG
    TTCTCTGGAC CAAAGGGTCC CCCTGGTCCC CAGGGAAAAG ATGGGGTACC TGGACACCCT
    GGACAGAGAG GAGAATTGGG TTTCCAAGGT CAGACAGGCC CACCTGGACC AGCTGGTGTC
    GTGGGTCCTC AGGGAAAGAC AGGAGAAGCA GGGCCTCTGG GTGAGAGGGG GCCCCCAGGG
    CCCCCTGGAC CTCCTGGTGA ACAAGGTCTT CCGGGCCTGG AAGGCAGAGA GGGGGCCAAG
    GGGGATGTGG GACCACTGGG ACCCCTTGGG AAGGAAGGGC CACCTGGACC CAGAGGTTTC
    CCTGGTCCTC ATGGAGCCCC CGGGGACCCA GGACCGACTG GTTTAAAGGG TGACAAGGGC
    CCCCCAGGTC CTGTTGGTGC CAACGGATCC CCTGGGGAGC GTGGTCCTGT AGGCCCAGCA
    GGGAGCATTG GGCTTCCTGG TCAAAGCGGA GGCCAAGGCC CCGTGGGCCC CACAGGCGAG
    AAGGGTTCCC CGGGTGAACG TGGCGCCCCT GGCCCAACTG GTAAAGATGG GATCCCCGGG
    CCCCTGGGGC TTCCTGGGCC CCCTGGAGCT GCGGGGCCTT CTGGCGAGGA AGGGGACAAG
    GGAGAAGTGG GTGCCCCCGG TCACAAAGGC AGCAAAGGTG ACAAGGGAGA TGCGGGCCCG
    CCTGGACCAA CAGGGATACG GGGTCTGGTG GGACACCCCG GCTCCCCGGG AGCAGACGGG
    GCTCAGGGAC GCCGGGGACC CCCAGGCCTC TTTGGGCAGA AAGGAGATGA TGGAGTGAGA
    GGCTTCCCTG GCATGATTGG TCCTCCTGGC CTACAGGGAA TGCCAGGCCC TGCTGGAGAA
    AAGGGAGAAG TTGGAGATGT AGGGTCCATG GGTCCCCACG GAGCTCCAGG CCCTCGGGGC
    CCCCAGGGCC CCAGCGGATC AGAGGGTCCT CCAGGGTTGC CTGGGGGAGT TGGTCAGCCT
    GGCGCTGTGG GGGAGAAGGG TGAGCCAGGA GAGCCTGGAG ACCCAGGACC CCCCGGGACC
    CCAGGCACCC CGGGCCCCAG GGGAGAAGTT GGTGAAAAGG GGGATACAGG CCCATCTGGG
    GCTGCTGGAC CCCCAGGCAA GAAAGGACCC CCTGGAGAGG ATGGAGCAAA GGGGAATGTG
    GGCCCCACAG GCCTCCCAGG AGACCTAGGC CCCCCCGGAG ACCCTGGAGT TTCGGGTCAA
    GATGGCCCCC CAGGGGAGAA GGGAAACCCT GGAGATGTGG GGGGACCGGG TCCACCTGGC
    GCTTCTGGGG AGCCTGGCGC CCCTGGGCCT CCTGGTAAAA GGGGTCCTTC TGGTCGCATG
    GGTCAAGAAG GTAGAGAAGG AGAGAAAGGT GCCAAGGGGG AACCAGGTCC TGATGGACCC
    CCAGGGAGGA CAGGCCCAGT AGGGGCTCGA GGGCCCCCTG GACGTGTTGG ACCTGAGGGT
    CTTCGTGGGA TCCCTGGACC TGTGGGCGAA CCAGGCCTCC TGGGACCTCC TGGACTGTTA
    GGCCCTCCTG GTCCCCTGGG GCCCTCTGGC CTACCAGGGC TGAAGGGAGA TGCTGGCCCC
    AAGGGAGAAA AGGGCCACAT TGGATTAATT GGTCTTGTCG GCCCCCCTGG GGAGGTTGGT
    GAGAAAGGGG ATCAGGGGTT GCCAGGAGTG CAAGGCTCCC CTGGCCTCCA GGGAGAACCG
    GGTTCTCCTG GCCCCATTGG CTCTCTGGGC CACCCTGGGC CCCAAGGCGT GGTGGGTCCT
    CCAGGACAGA AAGGCTCAAA GGGGTCCCCG GGATCCCAAG GTCCTCGTGG AGACGCTGGC
    CCCCCAGGTC CTCCTGGTCC CCCGGGTCCC TCTGCGGAGC TGCACGGGCT GCGCCGGCGC
    CGACGTTCCC TGCTAGGCTT GGGAACCCCG GAGGGCGGCC TGGAGGAGGT GCTGGCCTCG
    ATGGCGTCGC TGAGCTTCGA GTTAGAGCAG CTGCGGCGCC CTCCCGGCAC GGCTGAGCGC
    CCGGGCCTCG TGTGCAAAGA GTTGCACCGC AGCCATCCGC ATCTGCCAGA TGGGGAATAC
    TGGATTGATC CCAATCAAGG CTGCGCGCGG GATGCCCTCA GGGTTTTCTG TAACTTCACG
    GCAGGAGGAG AAACCTGCCT CTACCCAGAC AAGAAGTTTG AGACCGTGAA ACTGGCCTCT
    TGGTCCAAAG AAAAGCCAGG TGGCTGGTAT AGCACATTCC GTCGAGGGAA AAAGTTCTCC
    TACGTGGATG CCGATGGGGC CGCAGTGAGC GTGGTACAGC TGACCTTCCT GAAGTTGCTG
    AGTGCCACGG CCCGCCAGAG CTTCACATTC ACCTGCCAGA ACTCGGCCGC CTGGCTGGAC
    GAAGCTGCAG CTCCGGAAGG GACAGTCGAA GACCCTCTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849767.1
    CDS158..5179
    Translation

    Target ORF information:

    RefSeq Version XM_024993999.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type V alpha 3 chain (COL5A3), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993999.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCCGGAC GCAGGCCGTA GATCCTGTGG ATGTGCTGAA GGCACTGGGC
    ATGCGGGGGG GCCAGGCTGG GATCCCTGAG GGACCCGGCC TCTGCCCCCA GAGGGTCCCG
    GAGGGCGACC GAGCATTCCG GGTGGGCAAG ACTGGCACAC TTGGTGTCCC CACGCAGGAG
    CTCTTCCAAG ATGGGCACTT TCCTGAGAAC TTCTCCATCC TGATCACCCT GCGGGGCCAG
    CCGGCCAACC GTTCTGTCCT TCTGTCCATC TATGATGAGG GCGGCGTCCG GCAGCTCGGC
    TTGGCCTTGG GGCCAGCTCT GGACCTCCTA GGTGACTCCT TCAGCCCCCT CCCCCAGCAG
    GTCAACCTCA CGGATGGCAG GTGGCACCGT GTGGCAGTCA GTGTGGACGG TGGGATGGTG
    ACCCTGGTGG CTGATTGTGA ACCTCAGCTC CCCATGTTGG CTCAGGGACC TCGATTCATC
    AGTACAGCGG GACTCACTGT GCTGGGGACC CAGGACCTTG GCGAGGAGAC TTTTGAGGGA
    GATGTTCAGG AGCTGGTGAT AAGCCCAGAT CCCCAGGCTG CCTTCCAGGC ATGTGAGCGG
    TACCTTCCTG GCTGTGATGA CCTGGATCCA ACAGTCACCA CGGTCCCTCA GGGGGAGCCA
    GAAACCCCTC CCCCTAGGCG GAAGGGGAAA GGAAAAGGGA AGAAAAAAGG GCGAGGTCGT
    AAAGGGAAGG GCAGGAAGAA GAAGAACAAG GAAACTGTGG CTGTGAGTCC TCCTCCTGGC
    TCCCTGGACA ACCAGACCTC CACTGACATC CCCAAGACAG AGACACCAGA ACCAACTCTG
    TCTCCGACAC CCACGCCTTT GGTCATCACC ACAACTGTGA CCTTTGGTAG CAATGTCACC
    ATCCTAGAGG AGGGCCTGGA CCCTGACAAT GGAACTGAAC TGGAGACCCT GGAGACTGAG
    TTAGACAGAG AAGATGAAGA AGAAGGTGAT CCCACCATGG GCCCCCACTT CCGGGCAGCA
    GAACAGTCAT GGAATACTGA GTTCCAGATC TTTCCTGGTG CTGGGGAGAA AGGAGAAAAA
    GGAGAACCAG CTGTGGTCGA ACAGGGACAG CAGTTTGAAG GACCCCCAGG AGCCCCGGGA
    CCCCAAGGGG TGGTTGGCCC CTCAGGCCCT CCTGGCCCCC CAGGATTCCC TGGAGACCGT
    GGTCTACCGG GCCCTGCTGG CCTCCCAGGC ATCCCTGGCA TGGATGGGAT CCGGGGTCTC
    CCAGGCACTG TGATCATGAT GCCGTTCCAG TTTGCAAGAG GCTCTCACAA AGGACCCCCA
    GTATCCTTCC AGCAGGCCCA GGCTCAGGCA GTCCTGCAAC AAGCTCAGCT GTCTATGAAA
    GGCCCTCCTG GCCCGGTGGG GCTCACCGGA CGCCCAGGTC CCGTGGGTCT TCCTGGGTAT
    CCAGGTCTGA AAGGAGAGAT GGGAGAAATG GGGCCGCAGG GTCCCCGAGG GCTTCAGGGA
    CCTCATGGGC CCCCTGGCCG AGAGGGAAAG ATGGGCCGCG CTGGAGCAGA TGGGGCTCGG
    GGCCTCCCCG GCGCTACGGG ACCTAAGGGT GATCGGGGCT TTGATGGCCT GCCAGGGCTG
    CCTGGTGAGA AGGGCCAAAG GGGTGACTTT GGTTATGTGG GGAAACCTGG TCCCCCAGGA
    GAGGATGGAC GGAAGGGAGC AGAGGGACCT CCGGGGCCCA CTGGCCAGGC AGGGGAGCCG
    GGCCCCCGAG GACTGATTGG CCCTAGAGGC TCCCCCGGAC CCCTGGGACG CCCGGGTGTG
    ATTGGAAGTG ATGGTGCTCC AGGTGCCAAA GGAAATGTGG GTCCTCCAGG AGAACCAGGC
    CCCCCAGGAC AGCAGGGAAA TCCTGGGTCC CAGGGAATTC CCGGCCCCCA GGGACCCATC
    GGCACTCCTG GGGAGAAGGG TCCTCCTGGA AACCCAGGAA TTCCAGGCCT CCCAGGATCT
    GAAGGCCCTC CGGGTCACCC AGGTCATGAG GGCCCAACAG GAGAGAAAGG GGCCCAGGGT
    CCAGCAGGGT CAGCAGGCCC TCCGGGCTAT CCAGGACCTC GGGGTGTGAA GGGTACCTCT
    GGCAACCGGG GCCTCCAGGG GGAGAAAGGA GAGAAGGGAG AGGATGGTTT CCCAGGCTTC
    AAGGGTGATG CGGGGCTTAA AGGAGATCGG GGGCAACCCG GACCTCCAGG TCCTCGGGGA
    GAGGATGGTC CGGAGGGGCT AAAGGGGCAG GAGGGGCTGC TTGGCGAGGA GGGTCCCCCA
    GGCTCAGCTG GGGAGAAGGG CAAGCTTGGC GTGCCGGGTC TCCCAGGTTA TCCAGGACGT
    CCAGGCCCTA AGGGATCTAT TGGCTTTCCG GGACCTCTGG GACCATTAGG AGAAAAAGGG
    AAGCGGGGGA AGGCTGGGCA GCCAGGCCTG GAAGGAGAGC GGGGACCACC AGGTGCCCGT
    GGAGAGAGAG GGCAACCAGG TGCCACAGGG CAGCCGGGCC CCAAGGGTGA TGTGGGCCAG
    GACGGAGCCC CTGGGTTCCC TGGAGAAAAG GGCCTCCCAG GTCTGCAAGG TCCTCCTGGG
    TTCTCTGGAC CAAAGGGTCC CCCTGGTCCC CAGGGAAAAG ATGGGGTACC TGGACACCCT
    GGACAGAGAG GAGAATTGGG TTTCCAAGGT CAGACAGGCC CACCTGGACC AGCTGGTGTC
    GTGGGTCCTC AGGGAAAGAC AGGAGAAGCA GGGCCTCTGG GTGAGAGGGG GCCCCCAGGG
    CCCCCTGGAC CTCCTGGTGA ACAAGGTCTT CCGGGCCTGG AAGGCAGAGA GGGGGCCAAG
    GGGGATGTGG GACCACTGGG ACCCCTTGGG AAGGAAGGGC CACCTGGACC CAGAGGTTTC
    CCTGGTCCTC ATGGAGCCCC CGGGGACCCA GGACCGACTG GTTTAAAGGG TGACAAGGGC
    CCCCCAGGTC CTGTTGGTGC CAACGGATCC CCTGGGGAGC GTGGTCCTGT AGGCCCAGCA
    GGGAGCATTG GGCTTCCTGG TCAAAGCGGA GGCCAAGGCC CCGTGGGCCC CACAGGCGAG
    AAGGGTTCCC CGGGTGAACG TGGCGCCCCT GGCCCAACTG GTAAAGATGG GATCCCCGGG
    CCCCTGGGGC TTCCTGGGCC CCCTGGAGCT GCGGGGCCTT CTGGCGAGGA AGGGGACAAG
    GGAGAAGTGG GTGCCCCCGG TCACAAAGGC AGCAAAGGTG ACAAGGGAGA TGCGGGCCCG
    CCTGGACCAA CAGGGATACG GGGTCTGGTG GGACACCCCG GCTCCCCGGG AGCAGACGGG
    GCTCAGGGAC GCCGGGGACC CCCAGGCCTC TTTGGGCAGA AAGGAGATGA TGGAGTGAGA
    GGCTTCCCTG GCATGATTGG TCCTCCTGGC CTACAGGGAA TGCCAGGCCC TGCTGGAGAA
    AAGGGAGAAG TTGGAGATGT AGGGTCCATG GGTCCCCACG GAGCTCCAGG CCCTCGGGGC
    CCCCAGGGCC CCAGCGGATC AGAGGGTCCT CCAGGGTTGC CTGGGGGAGT TGGTCAGCCT
    GGCGCTGTGG GGGAGAAGGG TGAGCCAGGA GAGCCTGGAG ACCCAGGACC CCCCGGGACC
    CCAGGCACCC CGGGCCCCAG GGGAGAAGTT GGTGAAAAGG GGGATACAGG CCCATCTGGG
    GCTGCTGGAC CCCCAGGCAA GAAAGGACCC CCTGGAGAGG ATGGAGCAAA GGGGAATGTG
    GGCCCCACAG GCCTCCCAGG AGACCTAGGC CCCCCCGGAG ACCCTGGAGT TTCGGGTCAA
    GATGGCCCCC CAGGGGAGAA GGGAAACCCT GGAGATGTGG GGGGACCGGG TCCACCTGGC
    GCTTCTGGGG AGCCTGGCGC CCCTGGGCCT CCTGGTAAAA GGGGTCCTTC TGGTCGCATG
    GGTCAAGAAG GTAGAGAAGG AGAGAAAGGT GCCAAGGGGG AACCAGGTCC TGATGGACCC
    CCAGGGAGGA CAGGCCCAGT AGGGGCTCGA GGGCCCCCTG GACGTGTTGG ACCTGAGGGT
    CTTCGTGGGA TCCCTGGACC TGTGGGCGAA CCAGGCCTCC TGGGACCTCC TGGACTGTTA
    GGCCCTCCTG GTCCCCTGGG GCCCTCTGGC CTACCAGGGC TGAAGGGAGA TGCTGGCCCC
    AAGGGAGAAA AGGGCCACAT TGGATTAATT GGTCTTGTCG GCCCCCCTGG GGAGGTTGGT
    GAGAAAGGGG ATCAGGGGTT GCCAGGAGTG CAAGGCTCCC CTGGCCTCCA GGGAGAACCG
    GGTTCTCCTG GCCCCATTGG CTCTCTGGGC CACCCTGGGC CCCAAGGCGT GGTGGGTCCT
    CCAGGACAGA AAGGCTCAAA GGGGTCCCCG GGATCCCAAG GTCCTCGTGG AGACGCTGGC
    CCCCCAGGTC CTCCTGGTCC CCCGGGTCCC TCTGCGGAGC TGCACGGGCT GCGCCGGCGC
    CGACGTTCCC TGCTAGGCTT GGGAACCCCG GAGGGCGGCC TGGAGGAGGT GCTGGCCTCG
    ATGGCGTCGC TGAGCTTCGA GTTAGAGCAG CTGCGGCGCC CTCCCGGCAC GGCTGAGCGC
    CCGGGCCTCG TGTGCAAAGA GTTGCACCGC AGCCATCCGC ATCTGCCAGA TGGGGAATAC
    TGGATTGATC CCAATCAAGG CTGCGCGCGG GATGCCCTCA GGGTTTTCTG TAACTTCACG
    GCAGGAGGAG AAACCTGCCT CTACCCAGAC AAGAAGTTTG AGACCGTGAA ACTGGCCTCT
    TGGTCCAAAG AAAAGCCAGG TGGCTGGTAT AGCACATTCC GTCGAGGGAA AAAGTTCTCC
    TACGTGGATG CCGATGGGGC CGCAGTGAGC GTGGTACAGC TGACCTTCCT GAAGTTGCTG
    AGTGCCACGG CCCGCCAGAG CTTCACATTC ACCTGCCAGA ACTCGGCCGC CTGGCTGGAC
    GAAGCTGCAG CTCCGGAAGG GACAGTCGAA GACCCTCTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235874
    Clone ID Related Accession (Same CDS sequence) XM_024994000.1
    Accession Version XM_024994000.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-3(V) chain isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCCGGAC GCAGGCCGTA GATCCTGTGG ATGTGCTGAA GGCACTGGGC
    ATGCGGGGGG GCCAGGCTGG GATCCCTGAG GGACCCGGCC TCTGCCCCCA GAGGGTCCCG
    GAGGGCGACC GAGCATTCCG GGTGGGCAAG ACTGGCACAC TTGGTGTCCC CACGCAGGAG
    CTCTTCCAAG ATGGGCACTT TCCTGAGAAC TTCTCCATCC TGATCACCCT GCGGGGCCAG
    CCGGCCAACC GTTCTGTCCT TCTGTCCATC TATGATGAGG GCGGCGTCCG GCAGCTCGGC
    TTGGCCTTGG GGCCAGCTCT GGACCTCCTA GGTGACTCCT TCAGCCCCCT CCCCCAGCAG
    GTCAACCTCA CGGATGGCAG GTGGCACCGT GTGGCAGTCA GTGTGGACGG TGGGATGGTG
    ACCCTGGTGG CTGATTGTGA ACCTCAGCTC CCCATGTTGG CTCAGGGACC TCGATTCATC
    AGTACAGCGG GACTCACTGT GCTGGGGACC CAGGACCTTG GCGAGGAGAC TTTTGAGGGA
    GATGTTCAGG AGCTGGTGAT AAGCCCAGAT CCCCAGGCTG CCTTCCAGGC ATGTGAGCGG
    TACCTTCCTG GCTGTGATGA CCTGGATCCA ACAGTCACCA CGGTCCCTCA GGGGGAGCCA
    GAAACCCCTC CCCCTAGGCG GAAGGGGAAA GGAAAAGGGA AGAAAAAAGG GCGAGGTCGT
    AAAGGGAAGG GCAGGAAGAA GAAGAACAAG GAAACTGTGG CTGTGAGTCC TCCTCCTGGC
    TCCCTGGACA ACCAGACCTC CACTGACATC CCCAAGACAG AGACACCAGA ACCAACTCTG
    TCTCCGACAC CCACGCCTTT GGTCATCACC ACAACTGTGA CCTTTGGTAG CAATGTCACC
    ATCCTAGAGG AGGGCCTGGA CCCTGACAAT GGAACTGAAC TGGAGACCCT GGAGACTGAG
    TTAGACAGAG AAGATGAAGA AGAAGGTGAT CCCACCATGG GCCCCCACTT CCGGGCAGCA
    GAACAGTCAT GGAATACTGA GTTCCAGATC TTTCCTGGTG CTGGGGAGAA AGGAGAAAAA
    GGAGAACCAG CTGTGGTCGA ACAGGGACAG CAGTTTGAAG GACCCCCAGG AGCCCCGGGA
    CCCCAAGGGG TGGTTGGCCC CTCAGGCCCT CCTGGCCCCC CAGGATTCCC TGGAGACCGT
    GGTCTACCGG GCCCTGCTGG CCTCCCAGGC ATCCCTGGCA TGGATGGGAT CCGGGGTCTC
    CCAGGCACTG TGATCATGAT GCCGTTCCAG TTTGCAAGAG GCTCTCACAA AGGACCCCCA
    GTATCCTTCC AGCAGGCCCA GGCTCAGGCA GTCCTGCAAC AAGCTCAGCT GTCTATGAAA
    GGCCCTCCTG GCCCGGTGGG GCTCACCGGA CGCCCAGGTC CCGTGGGTCT TCCTGGGTAT
    CCAGGTCTGA AAGGAGAGAT GGGAGAAATG GGGCCGCAGG GTCCCCGAGG GCTTCAGGGA
    CCTCATGGGC CCCCTGGCCG AGAGGGAAAG ATGGGCCGCG CTGGAGCAGA TGGGGCTCGG
    GGCCTCCCCG GCGCTACGGG ACCTAAGGGT GATCGGGGCT TTGATGGCCT GCCAGGGCTG
    CCTGGTGAGA AGGGCCAAAG GGGTGACTTT GGTTATGTGG GGAAACCTGG TCCCCCAGGA
    GAGGATGGAC GGAAGGGAGC AGAGGGACCT CCGGGGCCCA CTGGCCAGGC AGGGGAGCCG
    GGCCCCCGAG GACTGATTGG CCCTAGAGGC TCCCCCGGAC CCCTGGGACG CCCGGGTGTG
    ATTGGAAGTG ATGGTGCTCC AGGTGCCAAA GGAAATGTGG GTCCTCCAGG AGAACCAGGC
    CCCCCAGGAC AGCAGGGAAA TCCTGGGTCC CAGGGAATTC CCGGCCCCCA GGGACCCATC
    GGCACTCCTG GGGAGAAGGG CAAGCTTGGC GTGCCGGGTC TCCCAGGTTA TCCAGGACGT
    CCAGGCCCTA AGGGATCTAT TGGCTTTCCG GGACCTCTGG GACCATTAGG AGAAAAAGGG
    AAGCGGGGGA AGGCTGGGCA GCCAGGCCTG GAAGGAGAGC GGGGACCACC AGGTGCCCGT
    GGAGAGAGAG GGCAACCAGG TGCCACAGGG CAGCCGGGCC CCAAGGGTGA TGTGGGCCAG
    GACGGAGCCC CTGGGTTCCC TGGAGAAAAG GGCCTCCCAG GTCTGCAAGG TCCTCCTGGG
    TTCTCTGGAC CAAAGGGTCC CCCTGGTCCC CAGGGAAAAG ATGGGGTACC TGGACACCCT
    GGACAGAGAG GAGAATTGGG TTTCCAAGGT CAGACAGGCC CACCTGGACC AGCTGGTGTC
    GTGGGTCCTC AGGGAAAGAC AGGAGAAGCA GGGCCTCTGG GTGAGAGGGG GCCCCCAGGG
    CCCCCTGGAC CTCCTGGTGA ACAAGGTCTT CCGGGCCTGG AAGGCAGAGA GGGGGCCAAG
    GGGGATGTGG GACCACTGGG ACCCCTTGGG AAGGAAGGGC CACCTGGACC CAGAGGTTTC
    CCTGGTCCTC ATGGAGCCCC CGGGGACCCA GGACCGACTG GTTTAAAGGG TGACAAGGGC
    CCCCCAGGTC CTGTTGGTGC CAACGGATCC CCTGGGGAGC GTGGTCCTGT AGGCCCAGCA
    GGGAGCATTG GGCTTCCTGG TCAAAGCGGA GGCCAAGGCC CCGTGGGCCC CACAGGCGAG
    AAGGGTTCCC CGGGTGAACG TGGCGCCCCT GGCCCAACTG GTAAAGATGG GATCCCCGGG
    CCCCTGGGGC TTCCTGGGCC CCCTGGAGCT GCGGGGCCTT CTGGCGAGGA AGGGGACAAG
    GGAGAAGTGG GTGCCCCCGG TCACAAAGGC AGCAAAGGTG ACAAGGGAGA TGCGGGCCCG
    CCTGGACCAA CAGGGATACG GGGTCTGGTG GGACACCCCG GCTCCCCGGG AGCAGACGGG
    GCTCAGGGAC GCCGGGGACC CCCAGGCCTC TTTGGGCAGA AAGGAGATGA TGGAGTGAGA
    GGCTTCCCTG GCATGATTGG TCCTCCTGGC CTACAGGGAA TGCCAGGCCC TGCTGGAGAA
    AAGGGAGAAG TTGGAGATGT AGGGTCCATG GGTCCCCACG GAGCTCCAGG CCCTCGGGGC
    CCCCAGGGCC CCAGCGGATC AGAGGGTCCT CCAGGGTTGC CTGGGGGAGT TGGTCAGCCT
    GGCGCTGTGG GGGAGAAGGG TGAGCCAGGA GAGCCTGGAG ACCCAGGACC CCCCGGGACC
    CCAGGCACCC CGGGCCCCAG GGGAGAAGTT GGTGAAAAGG GGGATACAGG CCCATCTGGG
    GCTGCTGGAC CCCCAGGCAA GAAAGGACCC CCTGGAGAGG ATGGAGCAAA GGGGAATGTG
    GGCCCCACAG GCCTCCCAGG AGACCTAGGC CCCCCCGGAG ACCCTGGAGT TTCGGGTCAA
    GATGGCCCCC CAGGGGAGAA GGGAAACCCT GGAGATGTGG GGGGACCGGG TCCACCTGGC
    GCTTCTGGGG AGCCTGGCGC CCCTGGGCCT CCTGGTAAAA GGGGTCCTTC TGGTCGCATG
    GGTCAAGAAG GTAGAGAAGG AGAGAAAGGT GCCAAGGGGG AACCAGGTCC TGATGGACCC
    CCAGGGAGGA CAGGCCCAGT AGGGGCTCGA GGGCCCCCTG GACGTGTTGG ACCTGAGGGT
    CTTCGTGGGA TCCCTGGACC TGTGGGCGAA CCAGGCCTCC TGGGACCTCC TGGACTGTTA
    GGCCCTCCTG GTCCCCTGGG GCCCTCTGGC CTACCAGGGC TGAAGGGAGA TGCTGGCCCC
    AAGGGAGAAA AGGGCCACAT TGGATTAATT GGTCTTGTCG GCCCCCCTGG GGAGGTTGGT
    GAGAAAGGGG ATCAGGGGTT GCCAGGAGTG CAAGGCTCCC CTGGCCTCCA GGGAGAACCG
    GGTTCTCCTG GCCCCATTGG CTCTCTGGGC CACCCTGGGC CCCAAGGCGT GGTGGGTCCT
    CCAGGACAGA AAGGCTCAAA GGGGTCCCCG GGATCCCAAG GTCCTCGTGG AGACGCTGGC
    CCCCCAGGTC CTCCTGGTCC CCCGGGTCCC TCTGCGGAGC TGCACGGGCT GCGCCGGCGC
    CGACGTTCCC TGCTAGGCTT GGGAACCCCG GAGGGCGGCC TGGAGGAGGT GCTGGCCTCG
    ATGGCGTCGC TGAGCTTCGA GTTAGAGCAG CTGCGGCGCC CTCCCGGCAC GGCTGAGCGC
    CCGGGCCTCG TGTGCAAAGA GTTGCACCGC AGCCATCCGC ATCTGCCAGA TGGGGAATAC
    TGGATTGATC CCAATCAAGG CTGCGCGCGG GATGCCCTCA GGGTTTTCTG TAACTTCACG
    GCAGGAGGAG AAACCTGCCT CTACCCAGAC AAGAAGTTTG AGACCGTGAA ACTGGCCTCT
    TGGTCCAAAG AAAAGCCAGG TGGCTGGTAT AGCACATTCC GTCGAGGGAA AAAGTTCTCC
    TACGTGGATG CCGATGGGGC CGCAGTGAGC GTGGTACAGC TGACCTTCCT GAAGTTGCTG
    AGTGCCACGG CCCGCCAGAG CTTCACATTC ACCTGCCAGA ACTCGGCCGC CTGGCTGGAC
    GAAGCTGCAG GTGACCACAG CCGCTCTGCC CGTTTCCTCG GGGCCAATGG GGAGGAGGTA
    TCCTTCAACC AGATGGCAGC CGCCACCATC ACAGTCCCCT ACGATGGCTG CAGGCTCCGG
    AAGGGACAGT CGAAGACCCT CTTTGAGTTC AGCTCCTCCC GAGTGGGATT TCTGCCCCTG
    TTGGACGTGG CAGCTGCTGA CTTTGGCCAG ACGAACCAGA AGTTTGGGTG GGTTCGCAGT
    GAAGGGACTG ATGTCAGACT ACAGAGGGAA GTGGGACTTC CCTGGACTGA GCTGCGTACT
    CTTTCTGCTG CCTCAGCCCA GCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849768.1
    CDS157..5103
    Translation

    Target ORF information:

    RefSeq Version XM_024994000.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type V alpha 3 chain (COL5A3), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994000.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCCGGAC GCAGGCCGTA GATCCTGTGG ATGTGCTGAA GGCACTGGGC
    ATGCGGGGGG GCCAGGCTGG GATCCCTGAG GGACCCGGCC TCTGCCCCCA GAGGGTCCCG
    GAGGGCGACC GAGCATTCCG GGTGGGCAAG ACTGGCACAC TTGGTGTCCC CACGCAGGAG
    CTCTTCCAAG ATGGGCACTT TCCTGAGAAC TTCTCCATCC TGATCACCCT GCGGGGCCAG
    CCGGCCAACC GTTCTGTCCT TCTGTCCATC TATGATGAGG GCGGCGTCCG GCAGCTCGGC
    TTGGCCTTGG GGCCAGCTCT GGACCTCCTA GGTGACTCCT TCAGCCCCCT CCCCCAGCAG
    GTCAACCTCA CGGATGGCAG GTGGCACCGT GTGGCAGTCA GTGTGGACGG TGGGATGGTG
    ACCCTGGTGG CTGATTGTGA ACCTCAGCTC CCCATGTTGG CTCAGGGACC TCGATTCATC
    AGTACAGCGG GACTCACTGT GCTGGGGACC CAGGACCTTG GCGAGGAGAC TTTTGAGGGA
    GATGTTCAGG AGCTGGTGAT AAGCCCAGAT CCCCAGGCTG CCTTCCAGGC ATGTGAGCGG
    TACCTTCCTG GCTGTGATGA CCTGGATCCA ACAGTCACCA CGGTCCCTCA GGGGGAGCCA
    GAAACCCCTC CCCCTAGGCG GAAGGGGAAA GGAAAAGGGA AGAAAAAAGG GCGAGGTCGT
    AAAGGGAAGG GCAGGAAGAA GAAGAACAAG GAAACTGTGG CTGTGAGTCC TCCTCCTGGC
    TCCCTGGACA ACCAGACCTC CACTGACATC CCCAAGACAG AGACACCAGA ACCAACTCTG
    TCTCCGACAC CCACGCCTTT GGTCATCACC ACAACTGTGA CCTTTGGTAG CAATGTCACC
    ATCCTAGAGG AGGGCCTGGA CCCTGACAAT GGAACTGAAC TGGAGACCCT GGAGACTGAG
    TTAGACAGAG AAGATGAAGA AGAAGGTGAT CCCACCATGG GCCCCCACTT CCGGGCAGCA
    GAACAGTCAT GGAATACTGA GTTCCAGATC TTTCCTGGTG CTGGGGAGAA AGGAGAAAAA
    GGAGAACCAG CTGTGGTCGA ACAGGGACAG CAGTTTGAAG GACCCCCAGG AGCCCCGGGA
    CCCCAAGGGG TGGTTGGCCC CTCAGGCCCT CCTGGCCCCC CAGGATTCCC TGGAGACCGT
    GGTCTACCGG GCCCTGCTGG CCTCCCAGGC ATCCCTGGCA TGGATGGGAT CCGGGGTCTC
    CCAGGCACTG TGATCATGAT GCCGTTCCAG TTTGCAAGAG GCTCTCACAA AGGACCCCCA
    GTATCCTTCC AGCAGGCCCA GGCTCAGGCA GTCCTGCAAC AAGCTCAGCT GTCTATGAAA
    GGCCCTCCTG GCCCGGTGGG GCTCACCGGA CGCCCAGGTC CCGTGGGTCT TCCTGGGTAT
    CCAGGTCTGA AAGGAGAGAT GGGAGAAATG GGGCCGCAGG GTCCCCGAGG GCTTCAGGGA
    CCTCATGGGC CCCCTGGCCG AGAGGGAAAG ATGGGCCGCG CTGGAGCAGA TGGGGCTCGG
    GGCCTCCCCG GCGCTACGGG ACCTAAGGGT GATCGGGGCT TTGATGGCCT GCCAGGGCTG
    CCTGGTGAGA AGGGCCAAAG GGGTGACTTT GGTTATGTGG GGAAACCTGG TCCCCCAGGA
    GAGGATGGAC GGAAGGGAGC AGAGGGACCT CCGGGGCCCA CTGGCCAGGC AGGGGAGCCG
    GGCCCCCGAG GACTGATTGG CCCTAGAGGC TCCCCCGGAC CCCTGGGACG CCCGGGTGTG
    ATTGGAAGTG ATGGTGCTCC AGGTGCCAAA GGAAATGTGG GTCCTCCAGG AGAACCAGGC
    CCCCCAGGAC AGCAGGGAAA TCCTGGGTCC CAGGGAATTC CCGGCCCCCA GGGACCCATC
    GGCACTCCTG GGGAGAAGGG CAAGCTTGGC GTGCCGGGTC TCCCAGGTTA TCCAGGACGT
    CCAGGCCCTA AGGGATCTAT TGGCTTTCCG GGACCTCTGG GACCATTAGG AGAAAAAGGG
    AAGCGGGGGA AGGCTGGGCA GCCAGGCCTG GAAGGAGAGC GGGGACCACC AGGTGCCCGT
    GGAGAGAGAG GGCAACCAGG TGCCACAGGG CAGCCGGGCC CCAAGGGTGA TGTGGGCCAG
    GACGGAGCCC CTGGGTTCCC TGGAGAAAAG GGCCTCCCAG GTCTGCAAGG TCCTCCTGGG
    TTCTCTGGAC CAAAGGGTCC CCCTGGTCCC CAGGGAAAAG ATGGGGTACC TGGACACCCT
    GGACAGAGAG GAGAATTGGG TTTCCAAGGT CAGACAGGCC CACCTGGACC AGCTGGTGTC
    GTGGGTCCTC AGGGAAAGAC AGGAGAAGCA GGGCCTCTGG GTGAGAGGGG GCCCCCAGGG
    CCCCCTGGAC CTCCTGGTGA ACAAGGTCTT CCGGGCCTGG AAGGCAGAGA GGGGGCCAAG
    GGGGATGTGG GACCACTGGG ACCCCTTGGG AAGGAAGGGC CACCTGGACC CAGAGGTTTC
    CCTGGTCCTC ATGGAGCCCC CGGGGACCCA GGACCGACTG GTTTAAAGGG TGACAAGGGC
    CCCCCAGGTC CTGTTGGTGC CAACGGATCC CCTGGGGAGC GTGGTCCTGT AGGCCCAGCA
    GGGAGCATTG GGCTTCCTGG TCAAAGCGGA GGCCAAGGCC CCGTGGGCCC CACAGGCGAG
    AAGGGTTCCC CGGGTGAACG TGGCGCCCCT GGCCCAACTG GTAAAGATGG GATCCCCGGG
    CCCCTGGGGC TTCCTGGGCC CCCTGGAGCT GCGGGGCCTT CTGGCGAGGA AGGGGACAAG
    GGAGAAGTGG GTGCCCCCGG TCACAAAGGC AGCAAAGGTG ACAAGGGAGA TGCGGGCCCG
    CCTGGACCAA CAGGGATACG GGGTCTGGTG GGACACCCCG GCTCCCCGGG AGCAGACGGG
    GCTCAGGGAC GCCGGGGACC CCCAGGCCTC TTTGGGCAGA AAGGAGATGA TGGAGTGAGA
    GGCTTCCCTG GCATGATTGG TCCTCCTGGC CTACAGGGAA TGCCAGGCCC TGCTGGAGAA
    AAGGGAGAAG TTGGAGATGT AGGGTCCATG GGTCCCCACG GAGCTCCAGG CCCTCGGGGC
    CCCCAGGGCC CCAGCGGATC AGAGGGTCCT CCAGGGTTGC CTGGGGGAGT TGGTCAGCCT
    GGCGCTGTGG GGGAGAAGGG TGAGCCAGGA GAGCCTGGAG ACCCAGGACC CCCCGGGACC
    CCAGGCACCC CGGGCCCCAG GGGAGAAGTT GGTGAAAAGG GGGATACAGG CCCATCTGGG
    GCTGCTGGAC CCCCAGGCAA GAAAGGACCC CCTGGAGAGG ATGGAGCAAA GGGGAATGTG
    GGCCCCACAG GCCTCCCAGG AGACCTAGGC CCCCCCGGAG ACCCTGGAGT TTCGGGTCAA
    GATGGCCCCC CAGGGGAGAA GGGAAACCCT GGAGATGTGG GGGGACCGGG TCCACCTGGC
    GCTTCTGGGG AGCCTGGCGC CCCTGGGCCT CCTGGTAAAA GGGGTCCTTC TGGTCGCATG
    GGTCAAGAAG GTAGAGAAGG AGAGAAAGGT GCCAAGGGGG AACCAGGTCC TGATGGACCC
    CCAGGGAGGA CAGGCCCAGT AGGGGCTCGA GGGCCCCCTG GACGTGTTGG ACCTGAGGGT
    CTTCGTGGGA TCCCTGGACC TGTGGGCGAA CCAGGCCTCC TGGGACCTCC TGGACTGTTA
    GGCCCTCCTG GTCCCCTGGG GCCCTCTGGC CTACCAGGGC TGAAGGGAGA TGCTGGCCCC
    AAGGGAGAAA AGGGCCACAT TGGATTAATT GGTCTTGTCG GCCCCCCTGG GGAGGTTGGT
    GAGAAAGGGG ATCAGGGGTT GCCAGGAGTG CAAGGCTCCC CTGGCCTCCA GGGAGAACCG
    GGTTCTCCTG GCCCCATTGG CTCTCTGGGC CACCCTGGGC CCCAAGGCGT GGTGGGTCCT
    CCAGGACAGA AAGGCTCAAA GGGGTCCCCG GGATCCCAAG GTCCTCGTGG AGACGCTGGC
    CCCCCAGGTC CTCCTGGTCC CCCGGGTCCC TCTGCGGAGC TGCACGGGCT GCGCCGGCGC
    CGACGTTCCC TGCTAGGCTT GGGAACCCCG GAGGGCGGCC TGGAGGAGGT GCTGGCCTCG
    ATGGCGTCGC TGAGCTTCGA GTTAGAGCAG CTGCGGCGCC CTCCCGGCAC GGCTGAGCGC
    CCGGGCCTCG TGTGCAAAGA GTTGCACCGC AGCCATCCGC ATCTGCCAGA TGGGGAATAC
    TGGATTGATC CCAATCAAGG CTGCGCGCGG GATGCCCTCA GGGTTTTCTG TAACTTCACG
    GCAGGAGGAG AAACCTGCCT CTACCCAGAC AAGAAGTTTG AGACCGTGAA ACTGGCCTCT
    TGGTCCAAAG AAAAGCCAGG TGGCTGGTAT AGCACATTCC GTCGAGGGAA AAAGTTCTCC
    TACGTGGATG CCGATGGGGC CGCAGTGAGC GTGGTACAGC TGACCTTCCT GAAGTTGCTG
    AGTGCCACGG CCCGCCAGAG CTTCACATTC ACCTGCCAGA ACTCGGCCGC CTGGCTGGAC
    GAAGCTGCAG GTGACCACAG CCGCTCTGCC CGTTTCCTCG GGGCCAATGG GGAGGAGGTA
    TCCTTCAACC AGATGGCAGC CGCCACCATC ACAGTCCCCT ACGATGGCTG CAGGCTCCGG
    AAGGGACAGT CGAAGACCCT CTTTGAGTTC AGCTCCTCCC GAGTGGGATT TCTGCCCCTG
    TTGGACGTGG CAGCTGCTGA CTTTGGCCAG ACGAACCAGA AGTTTGGGTG GGTTCGCAGT
    GAAGGGACTG ATGTCAGACT ACAGAGGGAA GTGGGACTTC CCTGGACTGA GCTGCGTACT
    CTTTCTGCTG CCTCAGCCCA GCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa216324
    Clone ID Related Accession (Same CDS sequence) NM_001205624.2
    Accession Version NM_001205624.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product collagen alpha-3(V) chain precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02019448.1 and DAAA02019447.1. On Jun 30, 2017 this sequence version replaced NM_001205624.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN02403639 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCGGGAC GCAGGCCGAT CCTGTGGATG TGCTGAAGGC ACTGGGCATG
    CGGGGGGGCC AGGCTGGGAT CCCTGAGGGA CCCGGCCTCT GCCCCCAGAG GGTCCCGGAG
    GGCGACCGAG CATTCCGGGT GGGCAAGACT GGCACACTTG GTGTCCCCAC GCAGGAGCTC
    TTCCAAGATG GGCACTTTCC TGAGAACTTC TCCATCCTGA TCACCCTGCG GGGCCAGCCG
    GCCAACCGTT CTGTCCTTCT GTCCATCTAT GATGAGGGCG GCGTCCGGCA GCTCGGCTTG
    GCCTTGGGGC CAGCTCTGGA CCTCCTAGGT GACTCCTTCA GCCCCCTCCC CCAGCAGGTC
    AACCTCACGG ATGGCAGGTG GCACCGTGTG GCAGTCAGTG TGGACGGTGG GATGGTGACC
    CTGGTGGCTG ATTGTGAACC TCAGCTCCCC ATGTTGGCTC AGGGACCTCG ATTCATCAGT
    ACAGCGGGAC TCACTGTGCT GGGGACCCAG GACCTTGGCG AGGAGACTTT TGAGGGAGAT
    GTTCAGGAGC TGGTGATAAG CCCAGATCCC CAGGCTGCCT TCCAGGCATG TGAGCGGTAC
    CTTCCTGGCT GTGATGACCT GGATCCAACA GTCACCACGG TCCCTCAGGG GGAGCCAGAA
    ACCCCTCCCC CTAGGCGGAA GGGGAAAGGA AAAGGGAAGA AAAAAGGGCG AGGTCGTAAA
    GGGAAGGGCA GGAAGAAGAA GAACAAGGAA ACTGTGGCTG TGAGTCCTCC TCCTGGCTCC
    CTGGACAACC AGACCTCCAC TGACATCCCC AAGACAGAGA CACCAGAACC AACTCTGTCT
    CCGACACCCA CGCCTTTGGT CATCACCACA ACTGTGACCT TTGGTAGCAA TGTCACCATC
    CTAGAGGAGG GCCTGGACCC TGACAATGGA ACTGAACTGG AGACCCTGGA GACTGAGTTA
    GACAGAGAAG ATGAAGAAGA AGGTGATCCC ACCATGGGCC CCCACTTCCG GGCAGCAGAA
    CAGTCATGGA ATACTGAGTT CCAGATCTTT CCTGGTGCTG GGGAGAAAGG AGAAAAAGGA
    GAACCAGCTG TGGTCGAACA GGGACAGCAG TTTGAAGGAC CCCCAGGAGC CCCGGGACCC
    CAAGGGGTGG TTGGCCCCTC AGGCCCTCCT GGCCCCCCAG GATTCCCTGG AGACCGTGGT
    CTACCGGGCC CTGCTGGCCT CCCAGGCATC CCTGGCATGG ATGGGATCCG GGGTCTCCCA
    GGCACTGTGA TCATGATGCC GTTCCAGTTT GCAAGAGGCT CTCACAAAGG ACCCCCAGTA
    TCCTTCCAGC AGGCCCAGGC TCAGGCAGTC CTGCAACAAG CTCAGCTGTC TATGAAAGGC
    CCTCCTGGCC CGGTGGGGCT CACCGGACGC CCAGGTCCCG TGGGTCTTCC TGGGTATCCA
    GGTCTGAAAG GAGAGATGGG AGAAATGGGG CCGCAGGGTC CCCGAGGGCT TCAGGGACCT
    CATGGGCCCC CTGGCCGAGA GGGAAAGATG GGCCGCGCTG GAGCAGATGG GGCTCGGGGC
    CTCCCCGGCG CTACGGGACC TAAGGGTGAT CGGGGCTTTG ATGGCCTGCC AGGGCTGCCT
    GGTGAGAAGG GCCAAAGGGG TGACTTTGGT TATGTGGGGA AACCTGGTCC CCCAGGAGAG
    GATGGACGGA AGGGAGCAGA GGGACCTCCG GGGCCCACTG GCCAGGCAGG GGAGCCGGGC
    CCCCGAGGAC TGATTGGCCC TAGAGGCTCC CCCGGACCCC TGGGACGCCC GGGTGTGATT
    GGAAGTGATG GTGCTCCAGG TGCCAAAGGA AATGTGGGTC CTCCAGGAGA ACCAGGCCCC
    CCAGGACAGC AGGGAAATCC TGGGTCCCAG GGAATTCCCG GCCCCCAGGG ACCCATCGGC
    ACTCCTGGGG AGAAGGGTCC TCCTGGAAAC CCAGGAATTC CAGGCCTCCC AGGATCTGAA
    GGCCCTCCGG GTCACCCAGG TCATGAGGGC CCAACAGGAG AGAAAGGGGC CCAGGGTCCA
    GCAGGGTCAG CAGGCCCTCC GGGCTATCCA GGACCTCGGG GTGTGAAGGG TACCTCTGGC
    AACCGGGGCC TCCAGGGGGA GAAAGGAGAG AAGGGAGAGG ATGGTTTCCC AGGCTTCAAG
    GGTGATGCGG GGCTTAAAGG AGATCGGGGG CAACCCGGAC CTCCAGGTCC TCGGGGAGAG
    GATGGTCCGG AGGGGCTAAA GGGGCAGGAG GGGCTGCTTG GCGAGGAGGG TCCCCCAGGC
    TCAGCTGGGG AGAAGGGCAA GCTTGGCGTG CCGGGTCTCC CAGGTTATCC AGGACGTCCA
    GGCCCTAAGG GATCTATTGG CTTTCCGGGA CCTCTGGGAC CATTAGGAGA AAAAGGGAAG
    CGGGGGAAGG CTGGGCAGCC AGGCCTGGAA GGAGAGCGGG GACCACCAGG TGCCCGTGGA
    GAGAGAGGGC AACCAGGTGC CACAGGGCAG CCGGGCCCCA AGGGTGATGT GGGCCAGGAC
    GGAGCCCCTG GGTTCCCTGG AGAAAAGGGC CTCCCAGGTC TGCAAGGTCC TCCTGGGTTC
    TCTGGACCAA AGGGTCCCCC TGGTCCCCAG GGAAAAGATG GGGTACCTGG ACACCCTGGA
    CAGAGAGGAG AATTGGGTTT CCAAGGTCAG ACAGGCCCAC CTGGACCAGC TGGTGTCGTG
    GGTCCTCAGG GAAAGACAGG AGAAGCAGGG CCTCTGGGTG AGAGGGGGCC CCCAGGGCCC
    CCTGGACCTC CTGGTGAACA AGGTCTTCCG GGCCTGGAAG GCAGAGAGGG GGCCAAGGGG
    GATGTGGGAC CACTGGGACC CCTTGGGAAG GAAGGGCCAC CTGGACCCAG AGGTTTCCCT
    GGTCCTCATG GAGCCCCCGG GGACCCAGGA CCGACTGGTT TAAAGGGTGA CAAGGGCCCC
    CCAGGTCCTG TTGGTGCCAA CGGATCCCCT GGGGAGCGTG GTCCTGTAGG CCCAGCAGGG
    AGCATTGGGC TTCCTGGTCA AAGCGGAGGC CAAGGCCCCG TGGGCCCCAC AGGCGAGAAG
    GGTTCCCCGG GTGAACGTGG CGCCCCTGGC CCAACTGGTA AAGATGGGAT CCCCGGGCCC
    CTGGGGCTTC CTGGGCCCCC TGGAGCTGCG GGGCCTTCTG GCGAGGAAGG GGACAAGGGA
    GAAGTGGGTG CCCCCGGTCA CAAAGGCAGC AAAGGTGACA AGGGAGATGC GGGCCCGCCT
    GGACCAACAG GGATACGGGG TCTGGTGGGA CACCCCGGCT CCCCGGGAGC AGACGGGGCT
    CAGGGACGCC GGGGACCCCC AGGCCTCTTT GGGCAGAAAG GAGATGATGG AGTGAGAGGC
    TTCCCTGGCA TGATTGGTCC TCCTGGCCTA CAGGGAATGC CAGGCCCTGC TGGAGAAAAG
    GGAGAAGTTG GAGATGTAGG GTCCATGGGT CCCCACGGAG CTCCAGGCCC TCGGGGCCCC
    CAGGGCCCCA GCGGATCAGA GGGTCCTCCA GGGTTGCCTG GGGGAGTTGG TCAGCCTGGC
    GCTGTGGGGG AGAAGGGTGA GCCAGGAGAG CCTGGAGACC CAGGACCCCC CGGGACCCCA
    GGCACCCCGG GCCCCAGGGG AGAAGTTGGT GAAAAGGGGG ATACAGGCCC ATCTGGGGCT
    GCTGGACCCC CAGGCAAGAA AGGACCCCCT GGAGAGGATG GAGCAAAGGG GAATGTGGGC
    CCCACAGGCC TCCCAGGAGA CCTAGGCCCC CCCGGAGACC CTGGAGTTTC GGGTCAAGAT
    GGCCCCCCAG GGGAGAAGGG AAACCCTGGA GATGTGGGGG GACCGGGTCC ACCTGGCGCT
    TCTGGGGAGC CTGGCGCCCC TGGGCCTCCT GGTAAAAGGG GTCCTTCTGG TCGCATGGGT
    CAAGAAGGTA GAGAAGGAGA GAAAGGTGCC AAGGGGGAAC CAGGTCCTGA TGGACCCCCA
    GGGAGGACAG GCCCAGTAGG GGCTCGAGGG CCCCCTGGAC GTGTTGGACC TGAGGGTCTT
    CGTGGGATCC CTGGACCTGT GGGCGAACCA GGCCTCCTGG GACCTCCTGG ACTGTTAGGC
    CCTCCTGGTC CCCTGGGGCC CTCTGGCCTA CCAGGGCTGA AGGGAGATGC TGGCCCCAAG
    GGAGAAAAGG GCCACATTGG ATTAATTGGT CTTGTCGGCC CCCCTGGGGA GGTTGGTGAG
    AAAGGGGATC AGGGGTTGCC AGGAGTGCAA GGCTCCCCTG GCCTCCAGGG AGAACCGGGT
    TCTCCTGGCC CCATTGGCTC TCTGGGCCAC CCTGGGCCCC AAGGCGTGGT GGGTCCTCCA
    GGACAGAAAG GCTCAAAGGG GTCCCCGGGA TCCCAAGGTC CTCGTGGAGA CGCTGGCCCC
    CCAGGTCCTC CTGGTCCCCC GGGTCCCTCT GCGGAGCTGC ACGGGCTGCG CCGGCGCCGA
    CGTTCCCTGC TAGGCTTGGG AACCCCGGAG GGCGGCCTGG AGGAGGTGCT GGCCTCGATG
    GCGTCGCTGA GCTTCGAGTT AGAGCAGCTG CGGCGCCCTC CCGGCACGGC TGAGCGCCCG
    GGCCTCGTGT GCAAAGAGTT GCACCGCAGC CATCCGCATC TGCCAGATGG GGAATACTGG
    ATTGATCCCA ATCAAGGCTG CGCGCGGGAT GCCCTCAGGG TTTTCTGTAA CTTCACGGCA
    GGAGGAGAAA CCTGCCTCTA CCCAGACAAG AAGTTTGAGA CCGTGAAACT GGCCTCTTGG
    TCCAAAGAAA AGCCAGGTGG CTGGTATAGC ACATTCCGTC GAGGGAAAAA GTTCTCCTAC
    GTGGATGCCG ATGGGGCCGC AGTGAGCGTG GTACAGCTGA CCTTCCTGAA GTTGCTGAGT
    GCCACGGCCC GCCAGAGCTT CACATTCACC TGCCAGAACT CGGCCGCCTG GCTGGACGAA
    GCTGCAGGTG ACCACAGCCG CTCTGCCCGT TTCCTCGGGG CCAATGGGGA GGAGGTATCC
    TTCAACCAGA TGGCAGCCGC CACCATCACA GTCCCCTACG ATGGCTGCAG GCTCCGGAAG
    GGACAGTCGA AGACCCTCTT TGAGTTCAGC TCCTCCCGAG TGGGATTTCT GCCCCTGTTG
    GACGTGGCAG CTGCTGACTT TGGCCAGACG AACCAGAAGT TTGGGTTTGA ACTGGGCCCT
    GTCTGCTTTA GCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192553.2
    CDS113..5350
    Translation

    Target ORF information:

    RefSeq Version NM_001205624.2
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type V alpha 3 chain (COL5A3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205624.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCGGGAC GCAGGCCGAT CCTGTGGATG TGCTGAAGGC ACTGGGCATG
    CGGGGGGGCC AGGCTGGGAT CCCTGAGGGA CCCGGCCTCT GCCCCCAGAG GGTCCCGGAG
    GGCGACCGAG CATTCCGGGT GGGCAAGACT GGCACACTTG GTGTCCCCAC GCAGGAGCTC
    TTCCAAGATG GGCACTTTCC TGAGAACTTC TCCATCCTGA TCACCCTGCG GGGCCAGCCG
    GCCAACCGTT CTGTCCTTCT GTCCATCTAT GATGAGGGCG GCGTCCGGCA GCTCGGCTTG
    GCCTTGGGGC CAGCTCTGGA CCTCCTAGGT GACTCCTTCA GCCCCCTCCC CCAGCAGGTC
    AACCTCACGG ATGGCAGGTG GCACCGTGTG GCAGTCAGTG TGGACGGTGG GATGGTGACC
    CTGGTGGCTG ATTGTGAACC TCAGCTCCCC ATGTTGGCTC AGGGACCTCG ATTCATCAGT
    ACAGCGGGAC TCACTGTGCT GGGGACCCAG GACCTTGGCG AGGAGACTTT TGAGGGAGAT
    GTTCAGGAGC TGGTGATAAG CCCAGATCCC CAGGCTGCCT TCCAGGCATG TGAGCGGTAC
    CTTCCTGGCT GTGATGACCT GGATCCAACA GTCACCACGG TCCCTCAGGG GGAGCCAGAA
    ACCCCTCCCC CTAGGCGGAA GGGGAAAGGA AAAGGGAAGA AAAAAGGGCG AGGTCGTAAA
    GGGAAGGGCA GGAAGAAGAA GAACAAGGAA ACTGTGGCTG TGAGTCCTCC TCCTGGCTCC
    CTGGACAACC AGACCTCCAC TGACATCCCC AAGACAGAGA CACCAGAACC AACTCTGTCT
    CCGACACCCA CGCCTTTGGT CATCACCACA ACTGTGACCT TTGGTAGCAA TGTCACCATC
    CTAGAGGAGG GCCTGGACCC TGACAATGGA ACTGAACTGG AGACCCTGGA GACTGAGTTA
    GACAGAGAAG ATGAAGAAGA AGGTGATCCC ACCATGGGCC CCCACTTCCG GGCAGCAGAA
    CAGTCATGGA ATACTGAGTT CCAGATCTTT CCTGGTGCTG GGGAGAAAGG AGAAAAAGGA
    GAACCAGCTG TGGTCGAACA GGGACAGCAG TTTGAAGGAC CCCCAGGAGC CCCGGGACCC
    CAAGGGGTGG TTGGCCCCTC AGGCCCTCCT GGCCCCCCAG GATTCCCTGG AGACCGTGGT
    CTACCGGGCC CTGCTGGCCT CCCAGGCATC CCTGGCATGG ATGGGATCCG GGGTCTCCCA
    GGCACTGTGA TCATGATGCC GTTCCAGTTT GCAAGAGGCT CTCACAAAGG ACCCCCAGTA
    TCCTTCCAGC AGGCCCAGGC TCAGGCAGTC CTGCAACAAG CTCAGCTGTC TATGAAAGGC
    CCTCCTGGCC CGGTGGGGCT CACCGGACGC CCAGGTCCCG TGGGTCTTCC TGGGTATCCA
    GGTCTGAAAG GAGAGATGGG AGAAATGGGG CCGCAGGGTC CCCGAGGGCT TCAGGGACCT
    CATGGGCCCC CTGGCCGAGA GGGAAAGATG GGCCGCGCTG GAGCAGATGG GGCTCGGGGC
    CTCCCCGGCG CTACGGGACC TAAGGGTGAT CGGGGCTTTG ATGGCCTGCC AGGGCTGCCT
    GGTGAGAAGG GCCAAAGGGG TGACTTTGGT TATGTGGGGA AACCTGGTCC CCCAGGAGAG
    GATGGACGGA AGGGAGCAGA GGGACCTCCG GGGCCCACTG GCCAGGCAGG GGAGCCGGGC
    CCCCGAGGAC TGATTGGCCC TAGAGGCTCC CCCGGACCCC TGGGACGCCC GGGTGTGATT
    GGAAGTGATG GTGCTCCAGG TGCCAAAGGA AATGTGGGTC CTCCAGGAGA ACCAGGCCCC
    CCAGGACAGC AGGGAAATCC TGGGTCCCAG GGAATTCCCG GCCCCCAGGG ACCCATCGGC
    ACTCCTGGGG AGAAGGGTCC TCCTGGAAAC CCAGGAATTC CAGGCCTCCC AGGATCTGAA
    GGCCCTCCGG GTCACCCAGG TCATGAGGGC CCAACAGGAG AGAAAGGGGC CCAGGGTCCA
    GCAGGGTCAG CAGGCCCTCC GGGCTATCCA GGACCTCGGG GTGTGAAGGG TACCTCTGGC
    AACCGGGGCC TCCAGGGGGA GAAAGGAGAG AAGGGAGAGG ATGGTTTCCC AGGCTTCAAG
    GGTGATGCGG GGCTTAAAGG AGATCGGGGG CAACCCGGAC CTCCAGGTCC TCGGGGAGAG
    GATGGTCCGG AGGGGCTAAA GGGGCAGGAG GGGCTGCTTG GCGAGGAGGG TCCCCCAGGC
    TCAGCTGGGG AGAAGGGCAA GCTTGGCGTG CCGGGTCTCC CAGGTTATCC AGGACGTCCA
    GGCCCTAAGG GATCTATTGG CTTTCCGGGA CCTCTGGGAC CATTAGGAGA AAAAGGGAAG
    CGGGGGAAGG CTGGGCAGCC AGGCCTGGAA GGAGAGCGGG GACCACCAGG TGCCCGTGGA
    GAGAGAGGGC AACCAGGTGC CACAGGGCAG CCGGGCCCCA AGGGTGATGT GGGCCAGGAC
    GGAGCCCCTG GGTTCCCTGG AGAAAAGGGC CTCCCAGGTC TGCAAGGTCC TCCTGGGTTC
    TCTGGACCAA AGGGTCCCCC TGGTCCCCAG GGAAAAGATG GGGTACCTGG ACACCCTGGA
    CAGAGAGGAG AATTGGGTTT CCAAGGTCAG ACAGGCCCAC CTGGACCAGC TGGTGTCGTG
    GGTCCTCAGG GAAAGACAGG AGAAGCAGGG CCTCTGGGTG AGAGGGGGCC CCCAGGGCCC
    CCTGGACCTC CTGGTGAACA AGGTCTTCCG GGCCTGGAAG GCAGAGAGGG GGCCAAGGGG
    GATGTGGGAC CACTGGGACC CCTTGGGAAG GAAGGGCCAC CTGGACCCAG AGGTTTCCCT
    GGTCCTCATG GAGCCCCCGG GGACCCAGGA CCGACTGGTT TAAAGGGTGA CAAGGGCCCC
    CCAGGTCCTG TTGGTGCCAA CGGATCCCCT GGGGAGCGTG GTCCTGTAGG CCCAGCAGGG
    AGCATTGGGC TTCCTGGTCA AAGCGGAGGC CAAGGCCCCG TGGGCCCCAC AGGCGAGAAG
    GGTTCCCCGG GTGAACGTGG CGCCCCTGGC CCAACTGGTA AAGATGGGAT CCCCGGGCCC
    CTGGGGCTTC CTGGGCCCCC TGGAGCTGCG GGGCCTTCTG GCGAGGAAGG GGACAAGGGA
    GAAGTGGGTG CCCCCGGTCA CAAAGGCAGC AAAGGTGACA AGGGAGATGC GGGCCCGCCT
    GGACCAACAG GGATACGGGG TCTGGTGGGA CACCCCGGCT CCCCGGGAGC AGACGGGGCT
    CAGGGACGCC GGGGACCCCC AGGCCTCTTT GGGCAGAAAG GAGATGATGG AGTGAGAGGC
    TTCCCTGGCA TGATTGGTCC TCCTGGCCTA CAGGGAATGC CAGGCCCTGC TGGAGAAAAG
    GGAGAAGTTG GAGATGTAGG GTCCATGGGT CCCCACGGAG CTCCAGGCCC TCGGGGCCCC
    CAGGGCCCCA GCGGATCAGA GGGTCCTCCA GGGTTGCCTG GGGGAGTTGG TCAGCCTGGC
    GCTGTGGGGG AGAAGGGTGA GCCAGGAGAG CCTGGAGACC CAGGACCCCC CGGGACCCCA
    GGCACCCCGG GCCCCAGGGG AGAAGTTGGT GAAAAGGGGG ATACAGGCCC ATCTGGGGCT
    GCTGGACCCC CAGGCAAGAA AGGACCCCCT GGAGAGGATG GAGCAAAGGG GAATGTGGGC
    CCCACAGGCC TCCCAGGAGA CCTAGGCCCC CCCGGAGACC CTGGAGTTTC GGGTCAAGAT
    GGCCCCCCAG GGGAGAAGGG AAACCCTGGA GATGTGGGGG GACCGGGTCC ACCTGGCGCT
    TCTGGGGAGC CTGGCGCCCC TGGGCCTCCT GGTAAAAGGG GTCCTTCTGG TCGCATGGGT
    CAAGAAGGTA GAGAAGGAGA GAAAGGTGCC AAGGGGGAAC CAGGTCCTGA TGGACCCCCA
    GGGAGGACAG GCCCAGTAGG GGCTCGAGGG CCCCCTGGAC GTGTTGGACC TGAGGGTCTT
    CGTGGGATCC CTGGACCTGT GGGCGAACCA GGCCTCCTGG GACCTCCTGG ACTGTTAGGC
    CCTCCTGGTC CCCTGGGGCC CTCTGGCCTA CCAGGGCTGA AGGGAGATGC TGGCCCCAAG
    GGAGAAAAGG GCCACATTGG ATTAATTGGT CTTGTCGGCC CCCCTGGGGA GGTTGGTGAG
    AAAGGGGATC AGGGGTTGCC AGGAGTGCAA GGCTCCCCTG GCCTCCAGGG AGAACCGGGT
    TCTCCTGGCC CCATTGGCTC TCTGGGCCAC CCTGGGCCCC AAGGCGTGGT GGGTCCTCCA
    GGACAGAAAG GCTCAAAGGG GTCCCCGGGA TCCCAAGGTC CTCGTGGAGA CGCTGGCCCC
    CCAGGTCCTC CTGGTCCCCC GGGTCCCTCT GCGGAGCTGC ACGGGCTGCG CCGGCGCCGA
    CGTTCCCTGC TAGGCTTGGG AACCCCGGAG GGCGGCCTGG AGGAGGTGCT GGCCTCGATG
    GCGTCGCTGA GCTTCGAGTT AGAGCAGCTG CGGCGCCCTC CCGGCACGGC TGAGCGCCCG
    GGCCTCGTGT GCAAAGAGTT GCACCGCAGC CATCCGCATC TGCCAGATGG GGAATACTGG
    ATTGATCCCA ATCAAGGCTG CGCGCGGGAT GCCCTCAGGG TTTTCTGTAA CTTCACGGCA
    GGAGGAGAAA CCTGCCTCTA CCCAGACAAG AAGTTTGAGA CCGTGAAACT GGCCTCTTGG
    TCCAAAGAAA AGCCAGGTGG CTGGTATAGC ACATTCCGTC GAGGGAAAAA GTTCTCCTAC
    GTGGATGCCG ATGGGGCCGC AGTGAGCGTG GTACAGCTGA CCTTCCTGAA GTTGCTGAGT
    GCCACGGCCC GCCAGAGCTT CACATTCACC TGCCAGAACT CGGCCGCCTG GCTGGACGAA
    GCTGCAGGTG ACCACAGCCG CTCTGCCCGT TTCCTCGGGG CCAATGGGGA GGAGGTATCC
    TTCAACCAGA TGGCAGCCGC CACCATCACA GTCCCCTACG ATGGCTGCAG GCTCCGGAAG
    GGACAGTCGA AGACCCTCTT TGAGTTCAGC TCCTCCCGAG TGGGATTTCT GCCCCTGTTG
    GACGTGGCAG CTGCTGACTT TGGCCAGACG AACCAGAAGT TTGGGTTTGA ACTGGGCCCT
    GTCTGCTTTA GCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40506
    Clone ID Related Accession (Same CDS sequence) NM_001205624.1 , NM_001205624.3
    Accession Version NM_001205624.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(Cattle)
    Product collagen alpha-3(V) chain precursor
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03050764.1 and AAFC03050763.1. On Apr 20, 2011 this sequence version replaced XM_584572.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120237 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCCGGAC GCAGGCCGAT CCTGTGGATG TGCTGAAGGC ACTGGGCATG
    CGGGGGGGCC AGGCTGGGAT CCCTGAGGGA CCCGGCCTCT GCCCCCAGAG GGTCCCGGAG
    GGCGACCGAG CATTCCGGGT GGGCAAGACT GGCACACTTG GTGTCCCCAC GCAGGAGCTC
    TTCCAAGATG GGCACTTTCC TGAGAACTTC TCCATCCTGA TCACCCTGCG GGGCCAGCCG
    GCCAACCGTT CTGTCCTTCT GTCCATCTAT GATGAGGGCG GCGTCCGGCA GCTCGGCTTG
    GCCTTGGGGC CAGCTCTGGA CCTCCTAGGT GACTCCTTCA GCCCCCTCCC CCAGCAGGTC
    AACCTCACGG ATGGCAGGTG GCACCGTGTG GCAGTCAGTG TGGACGGTGG GATGGTGACC
    CTGGTGGCTG ATTGTGAACC TCAGCTCCCC ATGTTGGCTC AGGGACCTCG ATTCATCAGT
    ACAGCGGGAC TCACTGTGCT GGGGACCCAG GACCTTGGCG AGGAGACTTT TGAGGGAGAT
    GTTCAGGAGC TGGTGATAAG CCCAGATCCC CAGGCTGCCT TCCAGGCATG TGAGCGGTAC
    CTTCCTGGCT GTGATGACCT GGATCCAACA GTCACCACGG TCCCTCAGGG GGAGCCAGAA
    ACCCCTCCCC CTAGGCGGAA GGGGAAAGGA AAAGGGAAGA AAAAAGGGCG AGGTCGTAAA
    GGGAAGGGCA GGAAGAAGAA GAACAAGGAA ACTGTGGCTG TGAGTCCTCC TCCTGGCTCC
    CTGGACAACC AGACCTCCAC TGACATCCCC AAGACAGAGA CACCAGAACC AACTCTGTCT
    CCGACACCCA CGCCTTTGGT CATCACCACA ACTGTGACCT TTGGTAGCAA TGTCACCATC
    CTAGAGGAGG GCCTGGACCC TGACAATGGA ACTGAACTGG AGACCCTGGA GACTGAGTTA
    GACAGAGAAG ATGAAGAAGA AGGTGATCCC ACCATGGGCC CCCACTTCCG GGCAGCAGAA
    CAGTCATGGA ATACTGAGTT CCAGATCTTT CCTGGTGCTG GGGAGAAAGG AGAAAAAGGA
    GAACCAGCTG TGGTCGAACA GGGACAGCAG TTTGAAGGAC CCCCAGGAGC CCCGGGACCC
    CAAGGGGTGG TTGGCCCCTC AGGCCCTCCT GGCCCCCCAG GATTCCCTGG AGACCGTGGT
    CTACCGGGCC CTGCTGGCCT CCCAGGCATC CCTGGCATGG ATGGGATCCG GGGTCTCCCA
    GGCACTGTGA TCATGATGCC GTTCCAGTTT GCAAGAGGCT CTCACAAAGG ACCCCCAGTA
    TCCTTCCAGC AGGCCCAGGC TCAGGCAGTC CTGCAACAAG CTCAGCTGTC TATGAAAGGC
    CCTCCTGGCC CGGTGGGGCT CACCGGACGC CCAGGTCCCG TGGGTCTTCC TGGGTATCCA
    GGTCTGAAAG GAGAGATGGG AGAAATGGGG CCGCAGGGTC CCCGAGGGCT TCAGGGACCT
    CATGGGCCCC CTGGCCGAGA GGGAAAGATG GGCCGCGCTG GAGCAGATGG GGCTCGGGGC
    CTCCCCGGCG CTACGGGACC TAAGGGTGAT CGGGGCTTTG ATGGCCTGCC AGGGCTGCCT
    GGTGAGAAGG GCCAAAGGGG TGACTTTGGT TATGTGGGGA AACCTGGTCC CCCAGGAGAG
    GATGGACGGA AGGGAGCAGA GGGACCTCCG GGGCCCACTG GCCAGGCAGG GGAGCCGGGC
    CCCCGAGGAC TGATTGGCCC TAGAGGCTCC CCCGGACCCC TGGGACGCCC GGGTGTGATT
    GGAAGTGATG GTGCTCCAGG TGCCAAAGGA AATGTGGGTC CTCCAGGAGA ACCAGGCCCC
    CCAGGACAGC AGGGAAATCC TGGGTCCCAG GGAATTCCCG GCCCCCAGGG ACCCATCGGC
    ACTCCTGGGG AGAAGGGTCC TCCTGGAAAC CCAGGAATTC CAGGCCTCCC AGGATCTGAA
    GGCCCTCCGG GTCACCCAGG TCATGAGGGC CCAACAGGAG AGAAAGGGGC CCAGGGTCCA
    GCAGGGTCAG CAGGCCCTCC GGGCTATCCA GGACCTCGGG GTGTGAAGGG TACCTCTGGC
    AACCGGGGCC TCCAGGGGGA GAAAGGAGAG AAGGGAGAGG ATGGTTTCCC AGGCTTCAAG
    GGTGATGCGG GGCTTAAAGG AGATCGGGGG CAACCCGGAC CTCCAGGTCC TCGGGGAGAG
    GATGGTCCGG AGGGGCTAAA GGGGCAGGAG GGGCTGCTTG GCGAGGAGGG TCCCCCAGGC
    TCAGCTGGGG AGAAGGGCAA GCTTGGCGTG CCGGGTCTCC CAGGTTATCC AGGACGTCCA
    GGCCCTAAGG GATCTATTGG CTTTCCGGGA CCTCTGGGAC CATTAGGAGA AAAAGGGAAG
    CGGGGGAAGG CTGGGCAGCC AGGCCTGGAA GGAGAGCGGG GACCACCAGG TGCCCGTGGA
    GAGAGAGGGC AACCAGGTGC CACAGGGCAG CCGGGCCCCA AGGGTGATGT GGGCCAGGAC
    GGAGCCCCTG GGTTCCCTGG AGAAAAGGGC CTCCCAGGTC TGCAAGGTCC TCCTGGGTTC
    TCTGGACCAA AGGGTCCCCC TGGTCCCCAG GGAAAAGATG GGGTACCTGG ACACCCTGGA
    CAGAGAGGAG AATTGGGTTT CCAAGGTCAG ACAGGCCCAC CTGGACCAGC TGGTGTCGTG
    GGTCCTCAGG GAAAGACAGG AGAAGCAGGG CCTCTGGGTG AGAGGGGGCC CCCAGGGCCC
    CCTGGACCTC CTGGTGAACA AGGTCTTCCG GGCCTGGAAG GCAGAGAGGG GGCCAAGGGG
    GATGTGGGAC CACTGGGACC CCTTGGGAAG GAAGGGCCAC CTGGACCCAG AGGTTTCCCT
    GGTCCTCATG GAGCCCCCGG GGACCCAGGA CCGACTGGTT TAAAGGGTGA CAAGGGCCCC
    CCAGGTCCTG TTGGTGCCAA CGGATCCCCT GGGGAGCGTG GTCCTGTAGG CCCAGCAGGG
    AGCATTGGGC TTCCTGGTCA AAGCGGAGGC CAAGGCCCCG TGGGCCCCAC AGGCGAGAAG
    GGTTCCCCGG GTGAACGTGG CGCCCCTGGC CCAACTGGTA AAGATGGGAT CCCCGGGCCC
    CTGGGGCTTC CTGGGCCCCC TGGAGCTGCG GGGCCTTCTG GCGAGGAAGG GGACAAGGGA
    GAAGTGGGTG CCCCCGGTCA CAAAGGCAGC AAAGGTGACA AGGGAGATGC GGGCCCGCCT
    GGACCAACAG GGATACGGGG TCTGGTGGGA CACCCCGGCT CCCCGGGAGC AGACGGGGCT
    CAGGGACGCC GGGGACCCCC AGGCCTCTTT GGGCAGAAAG GAGATGATGG AGTGAGAGGC
    TTCCCTGGCA TGATTGGTCC TCCTGGCCTA CAGGGAATGC CAGGCCCTGC TGGAGAAAAG
    GGAGAAGTTG GAGATGTAGG GTCCATGGGT CCCCACGGAG CTCCAGGCCC TCGGGGCCCC
    CAGGGCCCCA GCGGATCAGA GGGTCCTCCA GGGTTGCCTG GGGGAGTTGG TCAGCCTGGC
    GCTGTGGGGG AGAAGGGTGA GCCAGGAGAG CCTGGAGACC CAGGACCCCC CGGGACCCCA
    GGCACCCCGG GCCCCAGGGG AGAAGTTGGT GAAAAGGGGG ATACAGGCCC ATCTGGGGCT
    GCTGGACCCC CAGGCAAGAA AGGACCCCCT GGAGAGGATG GAGCAAAGGG GAATGTGGGC
    CCCACAGGCC TCCCAGGAGA CCTAGGCCCC CCCGGAGACC CTGGAGTTTC GGGTCAAGAT
    GGCCCCCCAG GGGAGAAGGG AAACCCTGGA GATGTGGGGG GACCGGGTCC ACCTGGCGCT
    TCTGGGGAGC CTGGCGCCCC TGGGCCTCCT GGTAAAAGGG GTCCTTCTGG TCGCATGGGT
    CAAGAAGGTA GAGAAGGAGA GAAAGGTGCC AAGGGGGAAC CAGGTCCTGA TGGACCCCCA
    GGGAGGACAG GCCCAGTAGG GGCTCGAGGG CCCCCTGGAC GTGTTGGACC TGAGGGTCTT
    CGTGGGATCC CTGGACCTGT GGGCGAACCA GGCCTCCTGG GACCTCCTGG ACTGTTAGGC
    CCTCCTGGTC CCCTGGGGCC CTCTGGCCTA CCAGGGCTGA AGGGAGATGC TGGCCCCAAG
    GGAGAAAAGG GCCACATTGG ATTAATTGGT CTTGTCGGCC CCCCTGGGGA GGTTGGTGAG
    AAAGGGGATC AGGGGTTGCC AGGAGTGCAA GGCTCCCCTG GCCTCCAGGG AGAACCGGGT
    TCTCCTGGCC CCATTGGCTC TCTGGGCCAC CCTGGGCCCC AAGGCGTGGT GGGTCCTCCA
    GGACAGAAAG GCTCAAAGGG GTCCCCGGGA TCCCAAGGTC CTCGTGGAGA CGCTGGCCCC
    CCAGGTCCTC CTGGTCCCCC GGGTCCCTCT GCGGAGCTGC ACGGGCTGCG CCGGCGCCGA
    CGTTCCCTGC TAGGCTTGGG AACCCCGGAG GGCGGCCTGG AGGAGGTGCT GGCCTCGATG
    GCGTCGCTGA GCTTCGAGTT AGAGCAGCTG CGGCGCCCTC CCGGCACGGC TGAGCGCCCG
    GGCCTCGTGT GCAAAGAGTT GCACCGCAGC CATCCGCATC TGCCAGATGG GGAATACTGG
    ATTGATCCCA ATCAAGGCTG CGCGCGGGAT GCCCTCAGGG TTTTCTGTAA CTTCACGGCA
    GGAGGAGAAA CCTGCCTCTA CCCAGACAAG AAGTTTGAGA CCGTGAAACT GGCCTCTTGG
    TCCAAAGAAA AGCCAGGTGG CTGGTATAGC ACATTCCGTC GAGGGAAAAA GTTCTCCTAC
    GTGGATGCCG ATGGGGCCGC AGTGAGCGTG GTACAGCTGA CCTTCCTGAA GTTGCTGAGT
    GCCACGGCCC GCCAGAGCTT CACATTCACC TGCCAGAACT CGGCCGCCTG GCTGGACGAA
    GCTGCAGGTG ACCACAGCCG CTCTGCCCGT TTCCTCGGGG CCAATGGGGA GGAGGTATCC
    TTCAACCAGA TGGCAGCCGC CACCATCACA GTCCCCTACG ATGGCTGCAG GCTCCGGAAG
    GGACAGTCGA AGACCCTCTT TGAGTTCAGC TCCTCCCGAG TGGGATTTCT GCCCCTGTTG
    GACGTGGCAG CTGCTGACTT TGGCCAGACG AACCAGAAGT TTGGGTTTGA ACTGGGCCCT
    GTCTGCTTTA GCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192553.1
    CDS113..5350
    Translation

    Target ORF information:

    RefSeq Version NM_001205624.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus collagen, type V, alpha 3 (COL5A3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205624.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCCGGAC GCAGGCCGAT CCTGTGGATG TGCTGAAGGC ACTGGGCATG
    CGGGGGGGCC AGGCTGGGAT CCCTGAGGGA CCCGGCCTCT GCCCCCAGAG GGTCCCGGAG
    GGCGACCGAG CATTCCGGGT GGGCAAGACT GGCACACTTG GTGTCCCCAC GCAGGAGCTC
    TTCCAAGATG GGCACTTTCC TGAGAACTTC TCCATCCTGA TCACCCTGCG GGGCCAGCCG
    GCCAACCGTT CTGTCCTTCT GTCCATCTAT GATGAGGGCG GCGTCCGGCA GCTCGGCTTG
    GCCTTGGGGC CAGCTCTGGA CCTCCTAGGT GACTCCTTCA GCCCCCTCCC CCAGCAGGTC
    AACCTCACGG ATGGCAGGTG GCACCGTGTG GCAGTCAGTG TGGACGGTGG GATGGTGACC
    CTGGTGGCTG ATTGTGAACC TCAGCTCCCC ATGTTGGCTC AGGGACCTCG ATTCATCAGT
    ACAGCGGGAC TCACTGTGCT GGGGACCCAG GACCTTGGCG AGGAGACTTT TGAGGGAGAT
    GTTCAGGAGC TGGTGATAAG CCCAGATCCC CAGGCTGCCT TCCAGGCATG TGAGCGGTAC
    CTTCCTGGCT GTGATGACCT GGATCCAACA GTCACCACGG TCCCTCAGGG GGAGCCAGAA
    ACCCCTCCCC CTAGGCGGAA GGGGAAAGGA AAAGGGAAGA AAAAAGGGCG AGGTCGTAAA
    GGGAAGGGCA GGAAGAAGAA GAACAAGGAA ACTGTGGCTG TGAGTCCTCC TCCTGGCTCC
    CTGGACAACC AGACCTCCAC TGACATCCCC AAGACAGAGA CACCAGAACC AACTCTGTCT
    CCGACACCCA CGCCTTTGGT CATCACCACA ACTGTGACCT TTGGTAGCAA TGTCACCATC
    CTAGAGGAGG GCCTGGACCC TGACAATGGA ACTGAACTGG AGACCCTGGA GACTGAGTTA
    GACAGAGAAG ATGAAGAAGA AGGTGATCCC ACCATGGGCC CCCACTTCCG GGCAGCAGAA
    CAGTCATGGA ATACTGAGTT CCAGATCTTT CCTGGTGCTG GGGAGAAAGG AGAAAAAGGA
    GAACCAGCTG TGGTCGAACA GGGACAGCAG TTTGAAGGAC CCCCAGGAGC CCCGGGACCC
    CAAGGGGTGG TTGGCCCCTC AGGCCCTCCT GGCCCCCCAG GATTCCCTGG AGACCGTGGT
    CTACCGGGCC CTGCTGGCCT CCCAGGCATC CCTGGCATGG ATGGGATCCG GGGTCTCCCA
    GGCACTGTGA TCATGATGCC GTTCCAGTTT GCAAGAGGCT CTCACAAAGG ACCCCCAGTA
    TCCTTCCAGC AGGCCCAGGC TCAGGCAGTC CTGCAACAAG CTCAGCTGTC TATGAAAGGC
    CCTCCTGGCC CGGTGGGGCT CACCGGACGC CCAGGTCCCG TGGGTCTTCC TGGGTATCCA
    GGTCTGAAAG GAGAGATGGG AGAAATGGGG CCGCAGGGTC CCCGAGGGCT TCAGGGACCT
    CATGGGCCCC CTGGCCGAGA GGGAAAGATG GGCCGCGCTG GAGCAGATGG GGCTCGGGGC
    CTCCCCGGCG CTACGGGACC TAAGGGTGAT CGGGGCTTTG ATGGCCTGCC AGGGCTGCCT
    GGTGAGAAGG GCCAAAGGGG TGACTTTGGT TATGTGGGGA AACCTGGTCC CCCAGGAGAG
    GATGGACGGA AGGGAGCAGA GGGACCTCCG GGGCCCACTG GCCAGGCAGG GGAGCCGGGC
    CCCCGAGGAC TGATTGGCCC TAGAGGCTCC CCCGGACCCC TGGGACGCCC GGGTGTGATT
    GGAAGTGATG GTGCTCCAGG TGCCAAAGGA AATGTGGGTC CTCCAGGAGA ACCAGGCCCC
    CCAGGACAGC AGGGAAATCC TGGGTCCCAG GGAATTCCCG GCCCCCAGGG ACCCATCGGC
    ACTCCTGGGG AGAAGGGTCC TCCTGGAAAC CCAGGAATTC CAGGCCTCCC AGGATCTGAA
    GGCCCTCCGG GTCACCCAGG TCATGAGGGC CCAACAGGAG AGAAAGGGGC CCAGGGTCCA
    GCAGGGTCAG CAGGCCCTCC GGGCTATCCA GGACCTCGGG GTGTGAAGGG TACCTCTGGC
    AACCGGGGCC TCCAGGGGGA GAAAGGAGAG AAGGGAGAGG ATGGTTTCCC AGGCTTCAAG
    GGTGATGCGG GGCTTAAAGG AGATCGGGGG CAACCCGGAC CTCCAGGTCC TCGGGGAGAG
    GATGGTCCGG AGGGGCTAAA GGGGCAGGAG GGGCTGCTTG GCGAGGAGGG TCCCCCAGGC
    TCAGCTGGGG AGAAGGGCAA GCTTGGCGTG CCGGGTCTCC CAGGTTATCC AGGACGTCCA
    GGCCCTAAGG GATCTATTGG CTTTCCGGGA CCTCTGGGAC CATTAGGAGA AAAAGGGAAG
    CGGGGGAAGG CTGGGCAGCC AGGCCTGGAA GGAGAGCGGG GACCACCAGG TGCCCGTGGA
    GAGAGAGGGC AACCAGGTGC CACAGGGCAG CCGGGCCCCA AGGGTGATGT GGGCCAGGAC
    GGAGCCCCTG GGTTCCCTGG AGAAAAGGGC CTCCCAGGTC TGCAAGGTCC TCCTGGGTTC
    TCTGGACCAA AGGGTCCCCC TGGTCCCCAG GGAAAAGATG GGGTACCTGG ACACCCTGGA
    CAGAGAGGAG AATTGGGTTT CCAAGGTCAG ACAGGCCCAC CTGGACCAGC TGGTGTCGTG
    GGTCCTCAGG GAAAGACAGG AGAAGCAGGG CCTCTGGGTG AGAGGGGGCC CCCAGGGCCC
    CCTGGACCTC CTGGTGAACA AGGTCTTCCG GGCCTGGAAG GCAGAGAGGG GGCCAAGGGG
    GATGTGGGAC CACTGGGACC CCTTGGGAAG GAAGGGCCAC CTGGACCCAG AGGTTTCCCT
    GGTCCTCATG GAGCCCCCGG GGACCCAGGA CCGACTGGTT TAAAGGGTGA CAAGGGCCCC
    CCAGGTCCTG TTGGTGCCAA CGGATCCCCT GGGGAGCGTG GTCCTGTAGG CCCAGCAGGG
    AGCATTGGGC TTCCTGGTCA AAGCGGAGGC CAAGGCCCCG TGGGCCCCAC AGGCGAGAAG
    GGTTCCCCGG GTGAACGTGG CGCCCCTGGC CCAACTGGTA AAGATGGGAT CCCCGGGCCC
    CTGGGGCTTC CTGGGCCCCC TGGAGCTGCG GGGCCTTCTG GCGAGGAAGG GGACAAGGGA
    GAAGTGGGTG CCCCCGGTCA CAAAGGCAGC AAAGGTGACA AGGGAGATGC GGGCCCGCCT
    GGACCAACAG GGATACGGGG TCTGGTGGGA CACCCCGGCT CCCCGGGAGC AGACGGGGCT
    CAGGGACGCC GGGGACCCCC AGGCCTCTTT GGGCAGAAAG GAGATGATGG AGTGAGAGGC
    TTCCCTGGCA TGATTGGTCC TCCTGGCCTA CAGGGAATGC CAGGCCCTGC TGGAGAAAAG
    GGAGAAGTTG GAGATGTAGG GTCCATGGGT CCCCACGGAG CTCCAGGCCC TCGGGGCCCC
    CAGGGCCCCA GCGGATCAGA GGGTCCTCCA GGGTTGCCTG GGGGAGTTGG TCAGCCTGGC
    GCTGTGGGGG AGAAGGGTGA GCCAGGAGAG CCTGGAGACC CAGGACCCCC CGGGACCCCA
    GGCACCCCGG GCCCCAGGGG AGAAGTTGGT GAAAAGGGGG ATACAGGCCC ATCTGGGGCT
    GCTGGACCCC CAGGCAAGAA AGGACCCCCT GGAGAGGATG GAGCAAAGGG GAATGTGGGC
    CCCACAGGCC TCCCAGGAGA CCTAGGCCCC CCCGGAGACC CTGGAGTTTC GGGTCAAGAT
    GGCCCCCCAG GGGAGAAGGG AAACCCTGGA GATGTGGGGG GACCGGGTCC ACCTGGCGCT
    TCTGGGGAGC CTGGCGCCCC TGGGCCTCCT GGTAAAAGGG GTCCTTCTGG TCGCATGGGT
    CAAGAAGGTA GAGAAGGAGA GAAAGGTGCC AAGGGGGAAC CAGGTCCTGA TGGACCCCCA
    GGGAGGACAG GCCCAGTAGG GGCTCGAGGG CCCCCTGGAC GTGTTGGACC TGAGGGTCTT
    CGTGGGATCC CTGGACCTGT GGGCGAACCA GGCCTCCTGG GACCTCCTGG ACTGTTAGGC
    CCTCCTGGTC CCCTGGGGCC CTCTGGCCTA CCAGGGCTGA AGGGAGATGC TGGCCCCAAG
    GGAGAAAAGG GCCACATTGG ATTAATTGGT CTTGTCGGCC CCCCTGGGGA GGTTGGTGAG
    AAAGGGGATC AGGGGTTGCC AGGAGTGCAA GGCTCCCCTG GCCTCCAGGG AGAACCGGGT
    TCTCCTGGCC CCATTGGCTC TCTGGGCCAC CCTGGGCCCC AAGGCGTGGT GGGTCCTCCA
    GGACAGAAAG GCTCAAAGGG GTCCCCGGGA TCCCAAGGTC CTCGTGGAGA CGCTGGCCCC
    CCAGGTCCTC CTGGTCCCCC GGGTCCCTCT GCGGAGCTGC ACGGGCTGCG CCGGCGCCGA
    CGTTCCCTGC TAGGCTTGGG AACCCCGGAG GGCGGCCTGG AGGAGGTGCT GGCCTCGATG
    GCGTCGCTGA GCTTCGAGTT AGAGCAGCTG CGGCGCCCTC CCGGCACGGC TGAGCGCCCG
    GGCCTCGTGT GCAAAGAGTT GCACCGCAGC CATCCGCATC TGCCAGATGG GGAATACTGG
    ATTGATCCCA ATCAAGGCTG CGCGCGGGAT GCCCTCAGGG TTTTCTGTAA CTTCACGGCA
    GGAGGAGAAA CCTGCCTCTA CCCAGACAAG AAGTTTGAGA CCGTGAAACT GGCCTCTTGG
    TCCAAAGAAA AGCCAGGTGG CTGGTATAGC ACATTCCGTC GAGGGAAAAA GTTCTCCTAC
    GTGGATGCCG ATGGGGCCGC AGTGAGCGTG GTACAGCTGA CCTTCCTGAA GTTGCTGAGT
    GCCACGGCCC GCCAGAGCTT CACATTCACC TGCCAGAACT CGGCCGCCTG GCTGGACGAA
    GCTGCAGGTG ACCACAGCCG CTCTGCCCGT TTCCTCGGGG CCAATGGGGA GGAGGTATCC
    TTCAACCAGA TGGCAGCCGC CACCATCACA GTCCCCTACG ATGGCTGCAG GCTCCGGAAG
    GGACAGTCGA AGACCCTCTT TGAGTTCAGC TCCTCCCGAG TGGGATTTCT GCCCCTGTTG
    GACGTGGCAG CTGCTGACTT TGGCCAGACG AACCAGAAGT TTGGGTTTGA ACTGGGCCCT
    GTCTGCTTTA GCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40506
    Clone ID Related Accession (Same CDS sequence) NM_001205624.1 , NM_001205624.3
    Accession Version NM_001205624.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product collagen alpha-3(V) chain precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000007.1. On May 30, 2018 this sequence version replaced NM_001205624.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCCGGAC GCAGGCCGAT CCTGTGGATG TGCTGAAGGC ACTGGGCATG
    CGGGGGGGCC AGGCTGGGAT CCCTGAGGGA CCCGGCCTCT GCCCCCAGAG GGTCCCGGAG
    GGCGACCGAG CATTCCGGGT GGGCAAGACT GGCACACTTG GTGTCCCCAC GCAGGAGCTC
    TTCCAAGATG GGCACTTTCC TGAGAACTTC TCCATCCTGA TCACCCTGCG GGGCCAGCCG
    GCCAACCGTT CTGTCCTTCT GTCCATCTAT GATGAGGGCG GCGTCCGGCA GCTCGGCTTG
    GCCTTGGGGC CAGCTCTGGA CCTCCTAGGT GACTCCTTCA GCCCCCTCCC CCAGCAGGTC
    AACCTCACGG ATGGCAGGTG GCACCGTGTG GCAGTCAGTG TGGACGGTGG GATGGTGACC
    CTGGTGGCTG ATTGTGAACC TCAGCTCCCC ATGTTGGCTC AGGGACCTCG ATTCATCAGT
    ACAGCGGGAC TCACTGTGCT GGGGACCCAG GACCTTGGCG AGGAGACTTT TGAGGGAGAT
    GTTCAGGAGC TGGTGATAAG CCCAGATCCC CAGGCTGCCT TCCAGGCATG TGAGCGGTAC
    CTTCCTGGCT GTGATGACCT GGATCCAACA GTCACCACGG TCCCTCAGGG GGAGCCAGAA
    ACCCCTCCCC CTAGGCGGAA GGGGAAAGGA AAAGGGAAGA AAAAAGGGCG AGGTCGTAAA
    GGGAAGGGCA GGAAGAAGAA GAACAAGGAA ACTGTGGCTG TGAGTCCTCC TCCTGGCTCC
    CTGGACAACC AGACCTCCAC TGACATCCCC AAGACAGAGA CACCAGAACC AACTCTGTCT
    CCGACACCCA CGCCTTTGGT CATCACCACA ACTGTGACCT TTGGTAGCAA TGTCACCATC
    CTAGAGGAGG GCCTGGACCC TGACAATGGA ACTGAACTGG AGACCCTGGA GACTGAGTTA
    GACAGAGAAG ATGAAGAAGA AGGTGATCCC ACCATGGGCC CCCACTTCCG GGCAGCAGAA
    CAGTCATGGA ATACTGAGTT CCAGATCTTT CCTGGTGCTG GGGAGAAAGG AGAAAAAGGA
    GAACCAGCTG TGGTCGAACA GGGACAGCAG TTTGAAGGAC CCCCAGGAGC CCCGGGACCC
    CAAGGGGTGG TTGGCCCCTC AGGCCCTCCT GGCCCCCCAG GATTCCCTGG AGACCGTGGT
    CTACCGGGCC CTGCTGGCCT CCCAGGCATC CCTGGCATGG ATGGGATCCG GGGTCTCCCA
    GGCACTGTGA TCATGATGCC GTTCCAGTTT GCAAGAGGCT CTCACAAAGG ACCCCCAGTA
    TCCTTCCAGC AGGCCCAGGC TCAGGCAGTC CTGCAACAAG CTCAGCTGTC TATGAAAGGC
    CCTCCTGGCC CGGTGGGGCT CACCGGACGC CCAGGTCCCG TGGGTCTTCC TGGGTATCCA
    GGTCTGAAAG GAGAGATGGG AGAAATGGGG CCGCAGGGTC CCCGAGGGCT TCAGGGACCT
    CATGGGCCCC CTGGCCGAGA GGGAAAGATG GGCCGCGCTG GAGCAGATGG GGCTCGGGGC
    CTCCCCGGCG CTACGGGACC TAAGGGTGAT CGGGGCTTTG ATGGCCTGCC AGGGCTGCCT
    GGTGAGAAGG GCCAAAGGGG TGACTTTGGT TATGTGGGGA AACCTGGTCC CCCAGGAGAG
    GATGGACGGA AGGGAGCAGA GGGACCTCCG GGGCCCACTG GCCAGGCAGG GGAGCCGGGC
    CCCCGAGGAC TGATTGGCCC TAGAGGCTCC CCCGGACCCC TGGGACGCCC GGGTGTGATT
    GGAAGTGATG GTGCTCCAGG TGCCAAAGGA AATGTGGGTC CTCCAGGAGA ACCAGGCCCC
    CCAGGACAGC AGGGAAATCC TGGGTCCCAG GGAATTCCCG GCCCCCAGGG ACCCATCGGC
    ACTCCTGGGG AGAAGGGTCC TCCTGGAAAC CCAGGAATTC CAGGCCTCCC AGGATCTGAA
    GGCCCTCCGG GTCACCCAGG TCATGAGGGC CCAACAGGAG AGAAAGGGGC CCAGGGTCCA
    GCAGGGTCAG CAGGCCCTCC GGGCTATCCA GGACCTCGGG GTGTGAAGGG TACCTCTGGC
    AACCGGGGCC TCCAGGGGGA GAAAGGAGAG AAGGGAGAGG ATGGTTTCCC AGGCTTCAAG
    GGTGATGCGG GGCTTAAAGG AGATCGGGGG CAACCCGGAC CTCCAGGTCC TCGGGGAGAG
    GATGGTCCGG AGGGGCTAAA GGGGCAGGAG GGGCTGCTTG GCGAGGAGGG TCCCCCAGGC
    TCAGCTGGGG AGAAGGGCAA GCTTGGCGTG CCGGGTCTCC CAGGTTATCC AGGACGTCCA
    GGCCCTAAGG GATCTATTGG CTTTCCGGGA CCTCTGGGAC CATTAGGAGA AAAAGGGAAG
    CGGGGGAAGG CTGGGCAGCC AGGCCTGGAA GGAGAGCGGG GACCACCAGG TGCCCGTGGA
    GAGAGAGGGC AACCAGGTGC CACAGGGCAG CCGGGCCCCA AGGGTGATGT GGGCCAGGAC
    GGAGCCCCTG GGTTCCCTGG AGAAAAGGGC CTCCCAGGTC TGCAAGGTCC TCCTGGGTTC
    TCTGGACCAA AGGGTCCCCC TGGTCCCCAG GGAAAAGATG GGGTACCTGG ACACCCTGGA
    CAGAGAGGAG AATTGGGTTT CCAAGGTCAG ACAGGCCCAC CTGGACCAGC TGGTGTCGTG
    GGTCCTCAGG GAAAGACAGG AGAAGCAGGG CCTCTGGGTG AGAGGGGGCC CCCAGGGCCC
    CCTGGACCTC CTGGTGAACA AGGTCTTCCG GGCCTGGAAG GCAGAGAGGG GGCCAAGGGG
    GATGTGGGAC CACTGGGACC CCTTGGGAAG GAAGGGCCAC CTGGACCCAG AGGTTTCCCT
    GGTCCTCATG GAGCCCCCGG GGACCCAGGA CCGACTGGTT TAAAGGGTGA CAAGGGCCCC
    CCAGGTCCTG TTGGTGCCAA CGGATCCCCT GGGGAGCGTG GTCCTGTAGG CCCAGCAGGG
    AGCATTGGGC TTCCTGGTCA AAGCGGAGGC CAAGGCCCCG TGGGCCCCAC AGGCGAGAAG
    GGTTCCCCGG GTGAACGTGG CGCCCCTGGC CCAACTGGTA AAGATGGGAT CCCCGGGCCC
    CTGGGGCTTC CTGGGCCCCC TGGAGCTGCG GGGCCTTCTG GCGAGGAAGG GGACAAGGGA
    GAAGTGGGTG CCCCCGGTCA CAAAGGCAGC AAAGGTGACA AGGGAGATGC GGGCCCGCCT
    GGACCAACAG GGATACGGGG TCTGGTGGGA CACCCCGGCT CCCCGGGAGC AGACGGGGCT
    CAGGGACGCC GGGGACCCCC AGGCCTCTTT GGGCAGAAAG GAGATGATGG AGTGAGAGGC
    TTCCCTGGCA TGATTGGTCC TCCTGGCCTA CAGGGAATGC CAGGCCCTGC TGGAGAAAAG
    GGAGAAGTTG GAGATGTAGG GTCCATGGGT CCCCACGGAG CTCCAGGCCC TCGGGGCCCC
    CAGGGCCCCA GCGGATCAGA GGGTCCTCCA GGGTTGCCTG GGGGAGTTGG TCAGCCTGGC
    GCTGTGGGGG AGAAGGGTGA GCCAGGAGAG CCTGGAGACC CAGGACCCCC CGGGACCCCA
    GGCACCCCGG GCCCCAGGGG AGAAGTTGGT GAAAAGGGGG ATACAGGCCC ATCTGGGGCT
    GCTGGACCCC CAGGCAAGAA AGGACCCCCT GGAGAGGATG GAGCAAAGGG GAATGTGGGC
    CCCACAGGCC TCCCAGGAGA CCTAGGCCCC CCCGGAGACC CTGGAGTTTC GGGTCAAGAT
    GGCCCCCCAG GGGAGAAGGG AAACCCTGGA GATGTGGGGG GACCGGGTCC ACCTGGCGCT
    TCTGGGGAGC CTGGCGCCCC TGGGCCTCCT GGTAAAAGGG GTCCTTCTGG TCGCATGGGT
    CAAGAAGGTA GAGAAGGAGA GAAAGGTGCC AAGGGGGAAC CAGGTCCTGA TGGACCCCCA
    GGGAGGACAG GCCCAGTAGG GGCTCGAGGG CCCCCTGGAC GTGTTGGACC TGAGGGTCTT
    CGTGGGATCC CTGGACCTGT GGGCGAACCA GGCCTCCTGG GACCTCCTGG ACTGTTAGGC
    CCTCCTGGTC CCCTGGGGCC CTCTGGCCTA CCAGGGCTGA AGGGAGATGC TGGCCCCAAG
    GGAGAAAAGG GCCACATTGG ATTAATTGGT CTTGTCGGCC CCCCTGGGGA GGTTGGTGAG
    AAAGGGGATC AGGGGTTGCC AGGAGTGCAA GGCTCCCCTG GCCTCCAGGG AGAACCGGGT
    TCTCCTGGCC CCATTGGCTC TCTGGGCCAC CCTGGGCCCC AAGGCGTGGT GGGTCCTCCA
    GGACAGAAAG GCTCAAAGGG GTCCCCGGGA TCCCAAGGTC CTCGTGGAGA CGCTGGCCCC
    CCAGGTCCTC CTGGTCCCCC GGGTCCCTCT GCGGAGCTGC ACGGGCTGCG CCGGCGCCGA
    CGTTCCCTGC TAGGCTTGGG AACCCCGGAG GGCGGCCTGG AGGAGGTGCT GGCCTCGATG
    GCGTCGCTGA GCTTCGAGTT AGAGCAGCTG CGGCGCCCTC CCGGCACGGC TGAGCGCCCG
    GGCCTCGTGT GCAAAGAGTT GCACCGCAGC CATCCGCATC TGCCAGATGG GGAATACTGG
    ATTGATCCCA ATCAAGGCTG CGCGCGGGAT GCCCTCAGGG TTTTCTGTAA CTTCACGGCA
    GGAGGAGAAA CCTGCCTCTA CCCAGACAAG AAGTTTGAGA CCGTGAAACT GGCCTCTTGG
    TCCAAAGAAA AGCCAGGTGG CTGGTATAGC ACATTCCGTC GAGGGAAAAA GTTCTCCTAC
    GTGGATGCCG ATGGGGCCGC AGTGAGCGTG GTACAGCTGA CCTTCCTGAA GTTGCTGAGT
    GCCACGGCCC GCCAGAGCTT CACATTCACC TGCCAGAACT CGGCCGCCTG GCTGGACGAA
    GCTGCAGGTG ACCACAGCCG CTCTGCCCGT TTCCTCGGGG CCAATGGGGA GGAGGTATCC
    TTCAACCAGA TGGCAGCCGC CACCATCACA GTCCCCTACG ATGGCTGCAG GCTCCGGAAG
    GGACAGTCGA AGACCCTCTT TGAGTTCAGC TCCTCCCGAG TGGGATTTCT GCCCCTGTTG
    GACGTGGCAG CTGCTGACTT TGGCCAGACG AACCAGAAGT TTGGGTTTGA ACTGGGCCCT
    GTCTGCTTTA GCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192553.3
    CDS113..5350
    Translation

    Target ORF information:

    RefSeq Version NM_001205624.3
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type V alpha 3 chain (COL5A3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205624.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCCGGAC GCAGGCCGAT CCTGTGGATG TGCTGAAGGC ACTGGGCATG
    CGGGGGGGCC AGGCTGGGAT CCCTGAGGGA CCCGGCCTCT GCCCCCAGAG GGTCCCGGAG
    GGCGACCGAG CATTCCGGGT GGGCAAGACT GGCACACTTG GTGTCCCCAC GCAGGAGCTC
    TTCCAAGATG GGCACTTTCC TGAGAACTTC TCCATCCTGA TCACCCTGCG GGGCCAGCCG
    GCCAACCGTT CTGTCCTTCT GTCCATCTAT GATGAGGGCG GCGTCCGGCA GCTCGGCTTG
    GCCTTGGGGC CAGCTCTGGA CCTCCTAGGT GACTCCTTCA GCCCCCTCCC CCAGCAGGTC
    AACCTCACGG ATGGCAGGTG GCACCGTGTG GCAGTCAGTG TGGACGGTGG GATGGTGACC
    CTGGTGGCTG ATTGTGAACC TCAGCTCCCC ATGTTGGCTC AGGGACCTCG ATTCATCAGT
    ACAGCGGGAC TCACTGTGCT GGGGACCCAG GACCTTGGCG AGGAGACTTT TGAGGGAGAT
    GTTCAGGAGC TGGTGATAAG CCCAGATCCC CAGGCTGCCT TCCAGGCATG TGAGCGGTAC
    CTTCCTGGCT GTGATGACCT GGATCCAACA GTCACCACGG TCCCTCAGGG GGAGCCAGAA
    ACCCCTCCCC CTAGGCGGAA GGGGAAAGGA AAAGGGAAGA AAAAAGGGCG AGGTCGTAAA
    GGGAAGGGCA GGAAGAAGAA GAACAAGGAA ACTGTGGCTG TGAGTCCTCC TCCTGGCTCC
    CTGGACAACC AGACCTCCAC TGACATCCCC AAGACAGAGA CACCAGAACC AACTCTGTCT
    CCGACACCCA CGCCTTTGGT CATCACCACA ACTGTGACCT TTGGTAGCAA TGTCACCATC
    CTAGAGGAGG GCCTGGACCC TGACAATGGA ACTGAACTGG AGACCCTGGA GACTGAGTTA
    GACAGAGAAG ATGAAGAAGA AGGTGATCCC ACCATGGGCC CCCACTTCCG GGCAGCAGAA
    CAGTCATGGA ATACTGAGTT CCAGATCTTT CCTGGTGCTG GGGAGAAAGG AGAAAAAGGA
    GAACCAGCTG TGGTCGAACA GGGACAGCAG TTTGAAGGAC CCCCAGGAGC CCCGGGACCC
    CAAGGGGTGG TTGGCCCCTC AGGCCCTCCT GGCCCCCCAG GATTCCCTGG AGACCGTGGT
    CTACCGGGCC CTGCTGGCCT CCCAGGCATC CCTGGCATGG ATGGGATCCG GGGTCTCCCA
    GGCACTGTGA TCATGATGCC GTTCCAGTTT GCAAGAGGCT CTCACAAAGG ACCCCCAGTA
    TCCTTCCAGC AGGCCCAGGC TCAGGCAGTC CTGCAACAAG CTCAGCTGTC TATGAAAGGC
    CCTCCTGGCC CGGTGGGGCT CACCGGACGC CCAGGTCCCG TGGGTCTTCC TGGGTATCCA
    GGTCTGAAAG GAGAGATGGG AGAAATGGGG CCGCAGGGTC CCCGAGGGCT TCAGGGACCT
    CATGGGCCCC CTGGCCGAGA GGGAAAGATG GGCCGCGCTG GAGCAGATGG GGCTCGGGGC
    CTCCCCGGCG CTACGGGACC TAAGGGTGAT CGGGGCTTTG ATGGCCTGCC AGGGCTGCCT
    GGTGAGAAGG GCCAAAGGGG TGACTTTGGT TATGTGGGGA AACCTGGTCC CCCAGGAGAG
    GATGGACGGA AGGGAGCAGA GGGACCTCCG GGGCCCACTG GCCAGGCAGG GGAGCCGGGC
    CCCCGAGGAC TGATTGGCCC TAGAGGCTCC CCCGGACCCC TGGGACGCCC GGGTGTGATT
    GGAAGTGATG GTGCTCCAGG TGCCAAAGGA AATGTGGGTC CTCCAGGAGA ACCAGGCCCC
    CCAGGACAGC AGGGAAATCC TGGGTCCCAG GGAATTCCCG GCCCCCAGGG ACCCATCGGC
    ACTCCTGGGG AGAAGGGTCC TCCTGGAAAC CCAGGAATTC CAGGCCTCCC AGGATCTGAA
    GGCCCTCCGG GTCACCCAGG TCATGAGGGC CCAACAGGAG AGAAAGGGGC CCAGGGTCCA
    GCAGGGTCAG CAGGCCCTCC GGGCTATCCA GGACCTCGGG GTGTGAAGGG TACCTCTGGC
    AACCGGGGCC TCCAGGGGGA GAAAGGAGAG AAGGGAGAGG ATGGTTTCCC AGGCTTCAAG
    GGTGATGCGG GGCTTAAAGG AGATCGGGGG CAACCCGGAC CTCCAGGTCC TCGGGGAGAG
    GATGGTCCGG AGGGGCTAAA GGGGCAGGAG GGGCTGCTTG GCGAGGAGGG TCCCCCAGGC
    TCAGCTGGGG AGAAGGGCAA GCTTGGCGTG CCGGGTCTCC CAGGTTATCC AGGACGTCCA
    GGCCCTAAGG GATCTATTGG CTTTCCGGGA CCTCTGGGAC CATTAGGAGA AAAAGGGAAG
    CGGGGGAAGG CTGGGCAGCC AGGCCTGGAA GGAGAGCGGG GACCACCAGG TGCCCGTGGA
    GAGAGAGGGC AACCAGGTGC CACAGGGCAG CCGGGCCCCA AGGGTGATGT GGGCCAGGAC
    GGAGCCCCTG GGTTCCCTGG AGAAAAGGGC CTCCCAGGTC TGCAAGGTCC TCCTGGGTTC
    TCTGGACCAA AGGGTCCCCC TGGTCCCCAG GGAAAAGATG GGGTACCTGG ACACCCTGGA
    CAGAGAGGAG AATTGGGTTT CCAAGGTCAG ACAGGCCCAC CTGGACCAGC TGGTGTCGTG
    GGTCCTCAGG GAAAGACAGG AGAAGCAGGG CCTCTGGGTG AGAGGGGGCC CCCAGGGCCC
    CCTGGACCTC CTGGTGAACA AGGTCTTCCG GGCCTGGAAG GCAGAGAGGG GGCCAAGGGG
    GATGTGGGAC CACTGGGACC CCTTGGGAAG GAAGGGCCAC CTGGACCCAG AGGTTTCCCT
    GGTCCTCATG GAGCCCCCGG GGACCCAGGA CCGACTGGTT TAAAGGGTGA CAAGGGCCCC
    CCAGGTCCTG TTGGTGCCAA CGGATCCCCT GGGGAGCGTG GTCCTGTAGG CCCAGCAGGG
    AGCATTGGGC TTCCTGGTCA AAGCGGAGGC CAAGGCCCCG TGGGCCCCAC AGGCGAGAAG
    GGTTCCCCGG GTGAACGTGG CGCCCCTGGC CCAACTGGTA AAGATGGGAT CCCCGGGCCC
    CTGGGGCTTC CTGGGCCCCC TGGAGCTGCG GGGCCTTCTG GCGAGGAAGG GGACAAGGGA
    GAAGTGGGTG CCCCCGGTCA CAAAGGCAGC AAAGGTGACA AGGGAGATGC GGGCCCGCCT
    GGACCAACAG GGATACGGGG TCTGGTGGGA CACCCCGGCT CCCCGGGAGC AGACGGGGCT
    CAGGGACGCC GGGGACCCCC AGGCCTCTTT GGGCAGAAAG GAGATGATGG AGTGAGAGGC
    TTCCCTGGCA TGATTGGTCC TCCTGGCCTA CAGGGAATGC CAGGCCCTGC TGGAGAAAAG
    GGAGAAGTTG GAGATGTAGG GTCCATGGGT CCCCACGGAG CTCCAGGCCC TCGGGGCCCC
    CAGGGCCCCA GCGGATCAGA GGGTCCTCCA GGGTTGCCTG GGGGAGTTGG TCAGCCTGGC
    GCTGTGGGGG AGAAGGGTGA GCCAGGAGAG CCTGGAGACC CAGGACCCCC CGGGACCCCA
    GGCACCCCGG GCCCCAGGGG AGAAGTTGGT GAAAAGGGGG ATACAGGCCC ATCTGGGGCT
    GCTGGACCCC CAGGCAAGAA AGGACCCCCT GGAGAGGATG GAGCAAAGGG GAATGTGGGC
    CCCACAGGCC TCCCAGGAGA CCTAGGCCCC CCCGGAGACC CTGGAGTTTC GGGTCAAGAT
    GGCCCCCCAG GGGAGAAGGG AAACCCTGGA GATGTGGGGG GACCGGGTCC ACCTGGCGCT
    TCTGGGGAGC CTGGCGCCCC TGGGCCTCCT GGTAAAAGGG GTCCTTCTGG TCGCATGGGT
    CAAGAAGGTA GAGAAGGAGA GAAAGGTGCC AAGGGGGAAC CAGGTCCTGA TGGACCCCCA
    GGGAGGACAG GCCCAGTAGG GGCTCGAGGG CCCCCTGGAC GTGTTGGACC TGAGGGTCTT
    CGTGGGATCC CTGGACCTGT GGGCGAACCA GGCCTCCTGG GACCTCCTGG ACTGTTAGGC
    CCTCCTGGTC CCCTGGGGCC CTCTGGCCTA CCAGGGCTGA AGGGAGATGC TGGCCCCAAG
    GGAGAAAAGG GCCACATTGG ATTAATTGGT CTTGTCGGCC CCCCTGGGGA GGTTGGTGAG
    AAAGGGGATC AGGGGTTGCC AGGAGTGCAA GGCTCCCCTG GCCTCCAGGG AGAACCGGGT
    TCTCCTGGCC CCATTGGCTC TCTGGGCCAC CCTGGGCCCC AAGGCGTGGT GGGTCCTCCA
    GGACAGAAAG GCTCAAAGGG GTCCCCGGGA TCCCAAGGTC CTCGTGGAGA CGCTGGCCCC
    CCAGGTCCTC CTGGTCCCCC GGGTCCCTCT GCGGAGCTGC ACGGGCTGCG CCGGCGCCGA
    CGTTCCCTGC TAGGCTTGGG AACCCCGGAG GGCGGCCTGG AGGAGGTGCT GGCCTCGATG
    GCGTCGCTGA GCTTCGAGTT AGAGCAGCTG CGGCGCCCTC CCGGCACGGC TGAGCGCCCG
    GGCCTCGTGT GCAAAGAGTT GCACCGCAGC CATCCGCATC TGCCAGATGG GGAATACTGG
    ATTGATCCCA ATCAAGGCTG CGCGCGGGAT GCCCTCAGGG TTTTCTGTAA CTTCACGGCA
    GGAGGAGAAA CCTGCCTCTA CCCAGACAAG AAGTTTGAGA CCGTGAAACT GGCCTCTTGG
    TCCAAAGAAA AGCCAGGTGG CTGGTATAGC ACATTCCGTC GAGGGAAAAA GTTCTCCTAC
    GTGGATGCCG ATGGGGCCGC AGTGAGCGTG GTACAGCTGA CCTTCCTGAA GTTGCTGAGT
    GCCACGGCCC GCCAGAGCTT CACATTCACC TGCCAGAACT CGGCCGCCTG GCTGGACGAA
    GCTGCAGGTG ACCACAGCCG CTCTGCCCGT TTCCTCGGGG CCAATGGGGA GGAGGTATCC
    TTCAACCAGA TGGCAGCCGC CACCATCACA GTCCCCTACG ATGGCTGCAG GCTCCGGAAG
    GGACAGTCGA AGACCCTCTT TGAGTTCAGC TCCTCCCGAG TGGGATTTCT GCCCCTGTTG
    GACGTGGCAG CTGCTGACTT TGGCCAGACG AACCAGAAGT TTGGGTTTGA ACTGGGCCCT
    GTCTGCTTTA GCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21009
    Clone ID Related Accession (Same CDS sequence) XM_005208735.3
    Accession Version XM_005208735.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-3(V) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005208735.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCGGGAC GCAGGCCGTA GATCCTGTGG ATGTGCTGAA GGCACTGGGC
    ATGCGGGGGG GCCAGGCTGG GATCCCTGAG GGACCCGGCC TCTGCCCCCA GAGGGTCCCG
    GAGGGCGACC GAGCATTCCG GGTGGGCAAG ACTGGCACAC TTGGTGTCCC CACGCAGGAG
    CTCTTCCAAG ATGGGCACTT TCCTGAGAAC TTCTCCATCC TGATCACCCT GCGGGGCCAG
    CCGGCCAACC GTTCTGTCCT TCTGTCCATC TATGATGAGG GCGGCGTCCG GCAGCTCGGC
    TTGGCCTTGG GGCCAGCTCT GGACCTCCTA GGTGACTCCT TCAGCCCCCT CCCCCAGCAG
    GTCAACCTCA CGGATGGCAG GTGGCACCGT GTGGCAGTCA GTGTGGACGG TGGGATGGTG
    ACCCTGGTGG CTGATTGTGA ACCTCAGCTC CCCATGTTGG CTCAGGGACC TCGATTCATC
    AGTACAGCGG GACTCACTGT GCTGGGGACC CAGGACCTTG GCGAGGAGAC TTTTGAGGGA
    GATGTTCAGG AGCTGGTGAT AAGCCCAGAT CCCCAGGCTG CCTTCCAGGC ATGTGAGCGG
    TACCTTCCTG GCTGTGATGA CCTGGATCCA ACAGTCACCA CGGTCCCTCA GGGGGAGCCA
    GAAACCCCTC CCCCTAGGCG GAAGGGGAAA GGAAAAGGGA AGAAAAAAGG GCGAGGTCGT
    AAAGGGAAGG GCAGGAAGAA GAAGAACAAG GAAACTGTGG CTGTGAGTCC TCCTCCTGGC
    TCCCTGGACA ACCAGACCTC CACTGACATC CCCAAGACAG AGACACCAGA ACCAACTCTG
    TCTCCGACAC CCACGCCTTT GGTCATCACC ACAACTGTGA CCTTTGGTAG CAATGTCACC
    ATCCTAGAGG AGGGCCTGGA CCCTGACAAT GGAACTGAAC TGGAGACCCT GGAGACTGAG
    TTAGACAGAG AAGATGAAGA AGAAGGTGAT CCCACCATGG GCCCCCACTT CCGGGCAGCA
    GAACAGTCAT GGAATACTGA GTTCCAGATC TTTCCTGGTG CTGGGGAGAA AGGAGAAAAA
    GGAGAACCAG CTGTGGTCGA ACAGGGACAG CAGTTTGAAG GACCCCCAGG AGCCCCGGGA
    CCCCAAGGGG TGGTTGGCCC CTCAGGCCCT CCTGGCCCCC CAGGATTCCC TGGAGACCGT
    GGTCTACCGG GCCCTGCTGG CCTCCCAGGC ATCCCTGGCA TGGATGGGAT CCGGGGTCTC
    CCAGGCACTG TGATCATGAT GCCGTTCCAG TTTGCAAGAG GCTCTCACAA AGGACCCCCA
    GTATCCTTCC AGCAGGCCCA GGCTCAGGCA GTCCTGCAAC AAGCTCAGCT GTCTATGAAA
    GGCCCTCCTG GCCCGGTGGG GCTCACCGGA CGCCCAGGTC CCGTGGGTCT TCCTGGGTAT
    CCAGGTCTGA AAGGAGAGAT GGGAGAAATG GGGCCGCAGG GTCCCCGAGG GCTTCAGGGA
    CCTCATGGGC CCCCTGGCCG AGAGGGAAAG ATGGGCCGCG CTGGAGCAGA TGGGGCTCGG
    GGCCTCCCCG GCGCTACGGG ACCTAAGGGT GATCGGGGCT TTGATGGCCT GCCAGGGCTG
    CCTGGTGAGA AGGGCCAAAG GGGTGACTTT GGTTATGTGG GGAAACCTGG TCCCCCAGGA
    GAGGATGGAC GGAAGGGAGC AGAGGGACCT CCGGGGCCCA CTGGCCAGGC AGGGGAGCCG
    GGCCCCCGAG GACTGATTGG CCCTAGAGGC TCCCCCGGAC CCCTGGGACG CCCGGGTGTG
    ATTGGAAGTG ATGGTGCTCC AGGTGCCAAA GGAAATGTGG GTCCTCCAGG AGAACCAGGC
    CCCCCAGGAC AGCAGGGAAA TCCTGGGTCC CAGGGAATTC CCGGCCCCCA GGGACCCATC
    GGCACTCCTG GGGAGAAGGG TCCTCCTGGA AACCCAGGAA TTCCAGGCCT CCCAGGATCT
    GAAGGCCCTC CGGGTCACCC AGGTCATGAG GGCCCAACAG GAGAGAAAGG GGCCCAGGGT
    CCAGCAGGGT CAGCAGGCCC TCCGGGCTAT CCAGGACCTC GGGGTGTGAA GGGTACCTCT
    GGCAACCGGG GCCTCCAGGG GGAGAAAGGA GAGAAGGGAG AGGATGGTTT CCCAGGCTTC
    AAGGGTGATG CGGGGCTTAA AGGAGATCGG GGGCAACCCG GACCTCCAGG TCCTCGGGGA
    GAGGATGGTC CGGAGGGGCT AAAGGGGCAG GAGGGGCTGC TTGGCGAGGA GGGTCCCCCA
    GGCTCAGCTG GGGAGAAGGG CAAGCTTGGC GTGCCGGGTC TCCCAGGTTA TCCAGGACGT
    CCAGGCCCTA AGGGATCTAT TGGCTTTCCG GGACCTCTGG GACCATTAGG AGAAAAAGGG
    AAGCGGGGGA AGGCTGGGCA GCCAGGCCTG GAAGGAGAGC GGGGACCACC AGGTGCCCGT
    GGAGAGAGAG GGCAACCAGG TGCCACAGGG CAGCCGGGCC CCAAGGGTGA TGTGGGCCAG
    GACGGAGCCC CTGGGTTCCC TGGAGAAAAG GGCCTCCCAG GTCTGCAAGG TCCTCCTGGG
    TTCTCTGGAC CAAAGGGTCC CCCTGGTCCC CAGGGAAAAG ATGGGGTACC TGGACACCCT
    GGACAGAGAG GAGAATTGGG TTTCCAAGGT CAGACAGGCC CACCTGGACC AGCTGGTGTC
    GTGGGTCCTC AGGGAAAGAC AGGAGAAGCA GGGCCTCTGG GTGAGAGGGG GCCCCCAGGG
    CCCCCTGGAC CTCCTGGTGA ACAAGGTCTT CCGGGCCTGG AAGGCAGAGA GGGGGCCAAG
    GGGGATGTGG GACCACTGGG ACCCCTTGGG AAGGAAGGGC CACCTGGACC CAGAGGTTTC
    CCTGGTCCTC ATGGAGCCCC CGGGGACCCA GGACCGACTG GTTTAAAGGG TGACAAGGGC
    CCCCCAGGTC CTGTTGGTGC CAACGGATCC CCTGGGGAGC GTGGTCCTGT AGGCCCAGCA
    GGGAGCATTG GGCTTCCTGG TCAAAGCGGA GGCCAAGGCC CCGTGGGCCC CACAGGCGAG
    AAGGGTTCCC CGGGTGAACG TGGCGCCCCT GGCCCAACTG GTAAAGATGG GATCCCCGGG
    CCCCTGGGGC TTCCTGGGCC CCCTGGAGCT GCGGGGCCTT CTGGCGAGGA AGGGGACAAG
    GGAGAAGTGG GTGCCCCCGG TCACAAAGGC AGCAAAGGTG ACAAGGGAGA TGCGGGCCCG
    CCTGGACCAA CAGGGATACG GGGTCTGGTG GGACACCCCG GCTCCCCGGG AGCAGACGGG
    GCTCAGGGAC GCCGGGGACC CCCAGGCCTC TTTGGGCAGA AAGGAGATGA TGGAGTGAGA
    GGCTTCCCTG GCATGATTGG TCCTCCTGGC CTACAGGGAA TGCCAGGCCC TGCTGGAGAA
    AAGGGAGAAG TTGGAGATGT AGGGTCCATG GGTCCCCACG GAGCTCCAGG CCCTCGGGGC
    CCCCAGGGCC CCAGCGGATC AGAGGGTCCT CCAGGGTTGC CTGGGGGAGT TGGTCAGCCT
    GGCGCTGTGG GGGAGAAGGG TGAGCCAGGA GAGCCTGGAG ACCCAGGACC CCCCGGGACC
    CCAGGCACCC CGGGCCCCAG GGGAGAAGTT GGTGAAAAGG GGGATACAGG CCCATCTGGG
    GCTGCTGGAC CCCCAGGCAA GAAAGGACCC CCTGGAGAGG ATGGAGCAAA GGGGAATGTG
    GGCCCCACAG GCCTCCCAGG AGACCTAGGC CCCCCCGGAG ACCCTGGAGT TTCGGGTCAA
    GATGGCCCCC CAGGGGAGAA GGGAAACCCT GGAGATGTGG GGGGACCGGG TCCACCTGGC
    GCTTCTGGGG AGCCTGGCGC CCCTGGGCCT CCTGGTAAAA GGGGTCCTTC TGGTCGCATG
    GGTCAAGAAG GTAGAGAAGG AGAGAAAGGT GCCAAGGGGG AACCAGGTCC TGATGGACCC
    CCAGGGAGGA CAGGCCCAGT AGGGGCTCGA GGGCCCCCTG GACGTGTTGG ACCTGAGGGT
    CTTCGTGGGA TCCCTGGACC TGTGGGCGAA CCAGGCCTCC TGGGACCTCC TGGACTGTTA
    GGCCCTCCTG GTCCCCTGGG GCCCTCTGGC CTACCAGGGC TGAAGGGAGA TGCTGGCCCC
    AAGGGAGAAA AGGGCCACAT TGGATTAATT GGTCTTGTCG GCCCCCCTGG GGAGGTTGGT
    GAGAAAGGGG ATCAGGGGTT GCCAGGAGTG CAAGGCTCCC CTGGCCTCCA GGGAGAACCG
    GGTTCTCCTG GCCCCATTGG CTCTCTGGGC CACCCTGGGC CCCAAGGCGT GGTGGGTCCT
    CCAGGACAGA AAGGCTCAAA GGGGTCCCCG GGATCCCAAG GTCCTCGTGG AGACGCTGGC
    CCCCCAGGTC CTCCTGGTCC CCCGGGTCCC TCTGCGGAGC TGCACGGGCT GCGCCGGCGC
    CGACGTTCCC TGCTAGGCTT GGGAACCCCG GAGGGCGGCC TGGAGGAGGT GCTGGCCTCG
    ATGGCGTCGC TGAGCTTCGA GTTAGAGCAG CTGCGGCGCC CTCCCGGCAC GGCTGAGCGC
    CCGGGCCTCG TGTGCAAAGA GTTGCACCGC AGCCATCCGC ATCTGCCAGA TGGGGAATAC
    TGGATTGATC CCAATCAAGG CTGCGCGCGG GATGCCCTCA GGGTTTTCTG TAACTTCACG
    GCAGGAGGAG AAACCTGCCT CTACCCAGAC AAGAAGTTTG AGACCGTGAA ACTGGCCTCT
    TGGTCCAAAG AAAAGCCAGG TGGCTGGTAT AGCACATTCC GTCGAGGGAA AAAGTTCTCC
    TACGTGGATG CCGATGGGGC CGCAGTGAGC GTGGTACAGC TGACCTTCCT GAAGTTGCTG
    AGTGCCACGG CCCGCCAGAG CTTCACATTC ACCTGCCAGA ACTCGGCCGC CTGGCTGGAC
    GAAGCTGCAG GTGACCACAG CCGCTCTGCC CGTTTCCTCG GGGCCAATGG GGAGGAGGTA
    TCCTTCAACC AGATGGCAGC CGCCACCATC ACAGTCCCCT ACGATGGCTG CAGGCTCCGG
    AAGGGACAGT CGAAGACCCT CTTTGAGTTC AGCTCCTCCC GAGTGGGATT TCTGCCCCTG
    TTGGACGTGG CAGCTGCTGA CTTTGGCCAG ACGAACCAGA AGTTTGGGTT TGAACTGGGC
    CCTGTCTGCT TTAGCAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005208792.1
    CDS153..5393
    Translation

    Target ORF information:

    RefSeq Version XM_005208735.3
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type V alpha 3 (COL5A3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005208735.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCGGGAC GCAGGCCGTA GATCCTGTGG ATGTGCTGAA GGCACTGGGC
    ATGCGGGGGG GCCAGGCTGG GATCCCTGAG GGACCCGGCC TCTGCCCCCA GAGGGTCCCG
    GAGGGCGACC GAGCATTCCG GGTGGGCAAG ACTGGCACAC TTGGTGTCCC CACGCAGGAG
    CTCTTCCAAG ATGGGCACTT TCCTGAGAAC TTCTCCATCC TGATCACCCT GCGGGGCCAG
    CCGGCCAACC GTTCTGTCCT TCTGTCCATC TATGATGAGG GCGGCGTCCG GCAGCTCGGC
    TTGGCCTTGG GGCCAGCTCT GGACCTCCTA GGTGACTCCT TCAGCCCCCT CCCCCAGCAG
    GTCAACCTCA CGGATGGCAG GTGGCACCGT GTGGCAGTCA GTGTGGACGG TGGGATGGTG
    ACCCTGGTGG CTGATTGTGA ACCTCAGCTC CCCATGTTGG CTCAGGGACC TCGATTCATC
    AGTACAGCGG GACTCACTGT GCTGGGGACC CAGGACCTTG GCGAGGAGAC TTTTGAGGGA
    GATGTTCAGG AGCTGGTGAT AAGCCCAGAT CCCCAGGCTG CCTTCCAGGC ATGTGAGCGG
    TACCTTCCTG GCTGTGATGA CCTGGATCCA ACAGTCACCA CGGTCCCTCA GGGGGAGCCA
    GAAACCCCTC CCCCTAGGCG GAAGGGGAAA GGAAAAGGGA AGAAAAAAGG GCGAGGTCGT
    AAAGGGAAGG GCAGGAAGAA GAAGAACAAG GAAACTGTGG CTGTGAGTCC TCCTCCTGGC
    TCCCTGGACA ACCAGACCTC CACTGACATC CCCAAGACAG AGACACCAGA ACCAACTCTG
    TCTCCGACAC CCACGCCTTT GGTCATCACC ACAACTGTGA CCTTTGGTAG CAATGTCACC
    ATCCTAGAGG AGGGCCTGGA CCCTGACAAT GGAACTGAAC TGGAGACCCT GGAGACTGAG
    TTAGACAGAG AAGATGAAGA AGAAGGTGAT CCCACCATGG GCCCCCACTT CCGGGCAGCA
    GAACAGTCAT GGAATACTGA GTTCCAGATC TTTCCTGGTG CTGGGGAGAA AGGAGAAAAA
    GGAGAACCAG CTGTGGTCGA ACAGGGACAG CAGTTTGAAG GACCCCCAGG AGCCCCGGGA
    CCCCAAGGGG TGGTTGGCCC CTCAGGCCCT CCTGGCCCCC CAGGATTCCC TGGAGACCGT
    GGTCTACCGG GCCCTGCTGG CCTCCCAGGC ATCCCTGGCA TGGATGGGAT CCGGGGTCTC
    CCAGGCACTG TGATCATGAT GCCGTTCCAG TTTGCAAGAG GCTCTCACAA AGGACCCCCA
    GTATCCTTCC AGCAGGCCCA GGCTCAGGCA GTCCTGCAAC AAGCTCAGCT GTCTATGAAA
    GGCCCTCCTG GCCCGGTGGG GCTCACCGGA CGCCCAGGTC CCGTGGGTCT TCCTGGGTAT
    CCAGGTCTGA AAGGAGAGAT GGGAGAAATG GGGCCGCAGG GTCCCCGAGG GCTTCAGGGA
    CCTCATGGGC CCCCTGGCCG AGAGGGAAAG ATGGGCCGCG CTGGAGCAGA TGGGGCTCGG
    GGCCTCCCCG GCGCTACGGG ACCTAAGGGT GATCGGGGCT TTGATGGCCT GCCAGGGCTG
    CCTGGTGAGA AGGGCCAAAG GGGTGACTTT GGTTATGTGG GGAAACCTGG TCCCCCAGGA
    GAGGATGGAC GGAAGGGAGC AGAGGGACCT CCGGGGCCCA CTGGCCAGGC AGGGGAGCCG
    GGCCCCCGAG GACTGATTGG CCCTAGAGGC TCCCCCGGAC CCCTGGGACG CCCGGGTGTG
    ATTGGAAGTG ATGGTGCTCC AGGTGCCAAA GGAAATGTGG GTCCTCCAGG AGAACCAGGC
    CCCCCAGGAC AGCAGGGAAA TCCTGGGTCC CAGGGAATTC CCGGCCCCCA GGGACCCATC
    GGCACTCCTG GGGAGAAGGG TCCTCCTGGA AACCCAGGAA TTCCAGGCCT CCCAGGATCT
    GAAGGCCCTC CGGGTCACCC AGGTCATGAG GGCCCAACAG GAGAGAAAGG GGCCCAGGGT
    CCAGCAGGGT CAGCAGGCCC TCCGGGCTAT CCAGGACCTC GGGGTGTGAA GGGTACCTCT
    GGCAACCGGG GCCTCCAGGG GGAGAAAGGA GAGAAGGGAG AGGATGGTTT CCCAGGCTTC
    AAGGGTGATG CGGGGCTTAA AGGAGATCGG GGGCAACCCG GACCTCCAGG TCCTCGGGGA
    GAGGATGGTC CGGAGGGGCT AAAGGGGCAG GAGGGGCTGC TTGGCGAGGA GGGTCCCCCA
    GGCTCAGCTG GGGAGAAGGG CAAGCTTGGC GTGCCGGGTC TCCCAGGTTA TCCAGGACGT
    CCAGGCCCTA AGGGATCTAT TGGCTTTCCG GGACCTCTGG GACCATTAGG AGAAAAAGGG
    AAGCGGGGGA AGGCTGGGCA GCCAGGCCTG GAAGGAGAGC GGGGACCACC AGGTGCCCGT
    GGAGAGAGAG GGCAACCAGG TGCCACAGGG CAGCCGGGCC CCAAGGGTGA TGTGGGCCAG
    GACGGAGCCC CTGGGTTCCC TGGAGAAAAG GGCCTCCCAG GTCTGCAAGG TCCTCCTGGG
    TTCTCTGGAC CAAAGGGTCC CCCTGGTCCC CAGGGAAAAG ATGGGGTACC TGGACACCCT
    GGACAGAGAG GAGAATTGGG TTTCCAAGGT CAGACAGGCC CACCTGGACC AGCTGGTGTC
    GTGGGTCCTC AGGGAAAGAC AGGAGAAGCA GGGCCTCTGG GTGAGAGGGG GCCCCCAGGG
    CCCCCTGGAC CTCCTGGTGA ACAAGGTCTT CCGGGCCTGG AAGGCAGAGA GGGGGCCAAG
    GGGGATGTGG GACCACTGGG ACCCCTTGGG AAGGAAGGGC CACCTGGACC CAGAGGTTTC
    CCTGGTCCTC ATGGAGCCCC CGGGGACCCA GGACCGACTG GTTTAAAGGG TGACAAGGGC
    CCCCCAGGTC CTGTTGGTGC CAACGGATCC CCTGGGGAGC GTGGTCCTGT AGGCCCAGCA
    GGGAGCATTG GGCTTCCTGG TCAAAGCGGA GGCCAAGGCC CCGTGGGCCC CACAGGCGAG
    AAGGGTTCCC CGGGTGAACG TGGCGCCCCT GGCCCAACTG GTAAAGATGG GATCCCCGGG
    CCCCTGGGGC TTCCTGGGCC CCCTGGAGCT GCGGGGCCTT CTGGCGAGGA AGGGGACAAG
    GGAGAAGTGG GTGCCCCCGG TCACAAAGGC AGCAAAGGTG ACAAGGGAGA TGCGGGCCCG
    CCTGGACCAA CAGGGATACG GGGTCTGGTG GGACACCCCG GCTCCCCGGG AGCAGACGGG
    GCTCAGGGAC GCCGGGGACC CCCAGGCCTC TTTGGGCAGA AAGGAGATGA TGGAGTGAGA
    GGCTTCCCTG GCATGATTGG TCCTCCTGGC CTACAGGGAA TGCCAGGCCC TGCTGGAGAA
    AAGGGAGAAG TTGGAGATGT AGGGTCCATG GGTCCCCACG GAGCTCCAGG CCCTCGGGGC
    CCCCAGGGCC CCAGCGGATC AGAGGGTCCT CCAGGGTTGC CTGGGGGAGT TGGTCAGCCT
    GGCGCTGTGG GGGAGAAGGG TGAGCCAGGA GAGCCTGGAG ACCCAGGACC CCCCGGGACC
    CCAGGCACCC CGGGCCCCAG GGGAGAAGTT GGTGAAAAGG GGGATACAGG CCCATCTGGG
    GCTGCTGGAC CCCCAGGCAA GAAAGGACCC CCTGGAGAGG ATGGAGCAAA GGGGAATGTG
    GGCCCCACAG GCCTCCCAGG AGACCTAGGC CCCCCCGGAG ACCCTGGAGT TTCGGGTCAA
    GATGGCCCCC CAGGGGAGAA GGGAAACCCT GGAGATGTGG GGGGACCGGG TCCACCTGGC
    GCTTCTGGGG AGCCTGGCGC CCCTGGGCCT CCTGGTAAAA GGGGTCCTTC TGGTCGCATG
    GGTCAAGAAG GTAGAGAAGG AGAGAAAGGT GCCAAGGGGG AACCAGGTCC TGATGGACCC
    CCAGGGAGGA CAGGCCCAGT AGGGGCTCGA GGGCCCCCTG GACGTGTTGG ACCTGAGGGT
    CTTCGTGGGA TCCCTGGACC TGTGGGCGAA CCAGGCCTCC TGGGACCTCC TGGACTGTTA
    GGCCCTCCTG GTCCCCTGGG GCCCTCTGGC CTACCAGGGC TGAAGGGAGA TGCTGGCCCC
    AAGGGAGAAA AGGGCCACAT TGGATTAATT GGTCTTGTCG GCCCCCCTGG GGAGGTTGGT
    GAGAAAGGGG ATCAGGGGTT GCCAGGAGTG CAAGGCTCCC CTGGCCTCCA GGGAGAACCG
    GGTTCTCCTG GCCCCATTGG CTCTCTGGGC CACCCTGGGC CCCAAGGCGT GGTGGGTCCT
    CCAGGACAGA AAGGCTCAAA GGGGTCCCCG GGATCCCAAG GTCCTCGTGG AGACGCTGGC
    CCCCCAGGTC CTCCTGGTCC CCCGGGTCCC TCTGCGGAGC TGCACGGGCT GCGCCGGCGC
    CGACGTTCCC TGCTAGGCTT GGGAACCCCG GAGGGCGGCC TGGAGGAGGT GCTGGCCTCG
    ATGGCGTCGC TGAGCTTCGA GTTAGAGCAG CTGCGGCGCC CTCCCGGCAC GGCTGAGCGC
    CCGGGCCTCG TGTGCAAAGA GTTGCACCGC AGCCATCCGC ATCTGCCAGA TGGGGAATAC
    TGGATTGATC CCAATCAAGG CTGCGCGCGG GATGCCCTCA GGGTTTTCTG TAACTTCACG
    GCAGGAGGAG AAACCTGCCT CTACCCAGAC AAGAAGTTTG AGACCGTGAA ACTGGCCTCT
    TGGTCCAAAG AAAAGCCAGG TGGCTGGTAT AGCACATTCC GTCGAGGGAA AAAGTTCTCC
    TACGTGGATG CCGATGGGGC CGCAGTGAGC GTGGTACAGC TGACCTTCCT GAAGTTGCTG
    AGTGCCACGG CCCGCCAGAG CTTCACATTC ACCTGCCAGA ACTCGGCCGC CTGGCTGGAC
    GAAGCTGCAG GTGACCACAG CCGCTCTGCC CGTTTCCTCG GGGCCAATGG GGAGGAGGTA
    TCCTTCAACC AGATGGCAGC CGCCACCATC ACAGTCCCCT ACGATGGCTG CAGGCTCCGG
    AAGGGACAGT CGAAGACCCT CTTTGAGTTC AGCTCCTCCC GAGTGGGATT TCTGCCCCTG
    TTGGACGTGG CAGCTGCTGA CTTTGGCCAG ACGAACCAGA AGTTTGGGTT TGAACTGGGC
    CCTGTCTGCT TTAGCAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46992
    Clone ID Related Accession (Same CDS sequence) XM_010806810.2
    Accession Version XM_010806810.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-3(V) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806810.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCGGGAC GCAGGCCGTA GATCCTGTGG ATGTGCTGAA GGCACTGGGC
    ATGCGGGGGG GCCAGGCTGG GATCCCTGAG GGACCCGGCC TCTGCCCCCA GAGGGTCCCG
    GAGGGCGACC GAGCATTCCG GGTGGGCAAG ACTGGCACAC TTGGTGTCCC CACGCAGGAG
    CTCTTCCAAG ATGGGCACTT TCCTGAGAAC TTCTCCATCC TGATCACCCT GCGGGGCCAG
    CCGGCCAACC GTTCTGTCCT TCTGTCCATC TATGATGAGG GCGGCGTCCG GCAGCTCGGC
    TTGGCCTTGG GGCCAGCTCT GGACCTCCTA GGTGACTCCT TCAGCCCCCT CCCCCAGCAG
    GTCAACCTCA CGGATGGCAG GTGGCACCGT GTGGCAGTCA GTGTGGACGG TGGGATGGTG
    ACCCTGGTGG CTGATTGTGA ACCTCAGCTC CCCATGTTGG CTCAGGGACC TCGATTCATC
    AGTACAGCGG GACTCACTGT GCTGGGGACC CAGGACCTTG GCGAGGAGAC TTTTGAGGGA
    GATGTTCAGG AGCTGGTGAT AAGCCCAGAT CCCCAGGCTG CCTTCCAGGC ATGTGAGCGG
    TACCTTCCTG GCTGTGATGA CCTGGATCCA ACAGTCACCA CGGTCCCTCA GGGGGAGCCA
    GAAACCCCTC CCCCTAGGCG GAAGGGGAAA GGAAAAGGGA AGAAAAAAGG GCGAGGTCGT
    AAAGGGAAGG GCAGGAAGAA GAAGAACAAG GAAACTGTGG CTGTGAGTCC TCCTCCTGGC
    TCCCTGGACA ACCAGACCTC CACTGACATC CCCAAGACAG AGACACCAGA ACCAACTCTG
    TCTCCGACAC CCACGCCTTT GGTCATCACC ACAACTGTGA CCTTTGGTAG CAATGTCACC
    ATCCTAGAGG AGGGCCTGGA CCCTGACAAT GGAACTGAAC TGGAGACCCT GGAGACTGAG
    TTAGACAGAG AAGATGAAGA AGAAGGTGAT CCCACCATGG GCCCCCACTT CCGGGCAGCA
    GAACAGTCAT GGAATACTGA GTTCCAGATC TTTCCTGGTG CTGGGGAGAA AGGAGAAAAA
    GGAGAACCAG CTGTGGTCGA ACAGGGACAG CAGTTTGAAG GACCCCCAGG AGCCCCGGGA
    CCCCAAGGGG TGGTTGGCCC CTCAGGCCCT CCTGGCCCCC CAGGATTCCC TGGAGACCGT
    GGTCTACCGG GCCCTGCTGG CCTCCCAGGC ATCCCTGGCA TGGATGGGAT CCGGGGTCTC
    CCAGGCACTG TGATCATGAT GCCGTTCCAG TTTGCAAGAG GCTCTCACAA AGGACCCCCA
    GTATCCTTCC AGCAGGCCCA GGCTCAGGCA GTCCTGCAAC AAGCTCAGCT GTCTATGAAA
    GGCCCTCCTG GCCCGGTGGG GCTCACCGGA CGCCCAGGTC CCGTGGGTCT TCCTGGGTAT
    CCAGGTCTGA AAGGAGAGAT GGGAGAAATG GGGCCGCAGG GTCCCCGAGG GCTTCAGGGA
    CCTCATGGGC CCCCTGGCCG AGAGGGAAAG ATGGGCCGCG CTGGAGCAGA TGGGGCTCGG
    GGCCTCCCCG GCGCTACGGG ACCTAAGGGT GATCGGGGCT TTGATGGCCT GCCAGGGCTG
    CCTGGTGAGA AGGGCCAAAG GGGTGACTTT GGTTATGTGG GGAAACCTGG TCCCCCAGGA
    GAGGATGGAC GGAAGGGAGC AGAGGGACCT CCGGGGCCCA CTGGCCAGGC AGGGGAGCCG
    GGCCCCCGAG GACTGATTGG CCCTAGAGGC TCCCCCGGAC CCCTGGGACG CCCGGGTGTG
    ATTGGAAGTG ATGGTGCTCC AGGTGCCAAA GGAAATGTGG GTCCTCCAGG AGAACCAGGC
    CCCCCAGGAC AGCAGGGAAA TCCTGGGTCC CAGGGAATTC CCGGCCCCCA GGGACCCATC
    GGCACTCCTG GGGAGAAGGG TCCTCCTGGA AACCCAGGAA TTCCAGGCCT CCCAGGATCT
    GAAGGCCCTC CGGGTCACCC AGGTCATGAG GGCCCAACAG GAGAGAAAGG GGCCCAGGGT
    CCAGCAGGGT CAGCAGGCCC TCCGGGCTAT CCAGGACCTC GGGGTGTGAA GGGTACCTCT
    GGCAACCGGG GCCTCCAGGG GGAGAAAGGA GAGAAGGGAG AGGATGGTTT CCCAGGCTTC
    AAGGGTGATG CGGGGCTTAA AGGAGATCGG GGGCAACCCG GACCTCCAGG TCCTCGGGGA
    GAGGATGGTC CGGAGGGGCT AAAGGGGCAG GAGGGGCTGC TTGGCGAGGA GGGTCCCCCA
    GGCTCAGCTG GGGAGAAGGG CAAGCTTGGC GTGCCGGGTC TCCCAGGTTA TCCAGGACGT
    CCAGGCCCTA AGGGATCTAT TGGCTTTCCG GGACCTCTGG GACCATTAGG AGAAAAAGGG
    AAGCGGGGGA AGGCTGGGCA GCCAGGCCTG GAAGGAGAGC GGGGACCACC AGGTGCCCGT
    GGAGAGAGAG GGCAACCAGG TGCCACAGGG CAGCCGGGCC CCAAGGGTGA TGTGGGCCAG
    GACGGAGCCC CTGGGTTCCC TGGAGAAAAG GGCCTCCCAG GTCTGCAAGG TCCTCCTGGG
    TTCTCTGGAC CAAAGGGTCC CCCTGGTCCC CAGGGAAAAG ATGGGGTACC TGGACACCCT
    GGACAGAGAG GAGAATTGGG TTTCCAAGGT CAGACAGGCC CACCTGGACC AGCTGGTGTC
    GTGGGTCCTC AGGGAAAGAC AGGAGAAGCA GGGCCTCTGG GTGAGAGGGG GCCCCCAGGG
    CCCCCTGGAC CTCCTGGTGA ACAAGGTCTT CCGGGCCTGG AAGGCAGAGA GGGGGCCAAG
    GGGGATGTGG GACCACTGGG ACCCCTTGGG AAGGAAGGGC CACCTGGACC CAGAGGTTTC
    CCTGGTCCTC ATGGAGCCCC CGGGGACCCA GGACCGACTG GTTTAAAGGG TGACAAGGGC
    CCCCCAGGTC CTGTTGGTGC CAACGGATCC CCTGGGGAGC GTGGTCCTGT AGGCCCAGCA
    GGGAGCATTG GGCTTCCTGG TCAAAGCGGA GGCCAAGGCC CCGTGGGCCC CACAGGCGAG
    AAGGGTTCCC CGGGTGAACG TGGCGCCCCT GGCCCAACTG GTAAAGATGG GATCCCCGGG
    CCCCTGGGGC TTCCTGGGCC CCCTGGAGCT GCGGGGCCTT CTGGCGAGGA AGGGGACAAG
    GGAGAAGTGG GTGCCCCCGG TCACAAAGGC AGCAAAGGTG ACAAGGGAGA TGCGGGCCCG
    CCTGGACCAA CAGGGATACG GGGTCTGGTG GGACACCCCG GCTCCCCGGG AGCAGACGGG
    GCTCAGGGAC GCCGGGGACC CCCAGGCCTC TTTGGGCAGA AAGGAGATGA TGGAGTGAGA
    GGCTTCCCTG GCATGATTGG TCCTCCTGGC CTACAGGGAA TGCCAGGCCC TGCTGGAGAA
    AAGGGAGAAG TTGGAGATGT AGGGTCCATG GGTCCCCACG GAGCTCCAGG CCCTCGGGGC
    CCCCAGGGCC CCAGCGGATC AGAGGGTCCT CCAGGGTTGC CTGGGGGAGT TGGTCAGCCT
    GGCGCTGTGG GGGAGAAGGG TGAGCCAGGA GAGCCTGGAG ACCCAGGACC CCCCGGGACC
    CCAGGCACCC CGGGCCCCAG GGGAGAAGTT GGTGAAAAGG GGGATACAGG CCCATCTGGG
    GCTGCTGGAC CCCCAGGCAA GAAAGGACCC CCTGGAGAGG ATGGAGCAAA GGGGAATGTG
    GGCCCCACAG GCCTCCCAGG AGACCTAGGC CCCCCCGGAG ACCCTGGAGT TTCGGGTCAA
    GATGGCCCCC CAGGGGAGAA GGGAAACCCT GGAGATGTGG GGGGACCGGG TCCACCTGGC
    GCTTCTGGGG AGCCTGGCGC CCCTGGGCCT CCTGGTAAAA GGGGTCCTTC TGGTCGCATG
    GGTCAAGAAG GTAGAGAAGG AGAGAAAGGT GCCAAGGGGG AACCAGGTCC TGATGGACCC
    CCAGGGAGGA CAGGCCCAGT AGGGGCTCGA GGGCCCCCTG GACGTGTTGG ACCTGAGGGT
    CTTCGTGGGA TCCCTGGACC TGTGGGCGAA CCAGGCCTCC TGGGACCTCC TGGACTGTTA
    GGCCCTCCTG GTCCCCTGGG GCCCTCTGGC CTACCAGGGC TGAAGGGAGA TGCTGGCCCC
    AAGGGAGAAA AGGGCCACAT TGGATTAATT GGTCTTGTCG GCCCCCCTGG GGAGGTTGGT
    GAGAAAGGGG ATCAGGGGTT GCCAGGAGTG CAAGGCTCCC CTGGCCTCCA GGGAGAACCG
    GGTTCTCCTG GCCCCATTGG CTCTCTGGGC CACCCTGGGC CCCAAGGCGT GGTGGGTCCT
    CCAGGACAGA AAGGCTCAAA GGGGTCCCCG GGATCCCAAG GTCCTCGTGG AGACGCTGGC
    CCCCCAGGTC CTCCTGGTCC CCCGGGTCCC TCTGCGGAGC TGCACGGGCT GCGCCGGCGC
    CGACGTTCCC TGCTAGGCTT GGGAACCCCG GAGGGCGGCC TGGAGGAGGT GCTGGCCTCG
    ATGGCGTCGC TGAGCTTCGA GTTAGAGCAG CTGCGGCGCC CTCCCGGCAC GGCTGAGCGC
    CCGGGCCTCG TGTGCAAAGA GTTGCACCGC AGCCATCCGC ATCTGCCAGA TGGGGAATAC
    TGGATTGATC CCAATCAAGG CTGCGCGCGG GATGCCCTCA GGGTTTTCTG TAACTTCACG
    GCAGGAGGAG AAACCTGCCT CTACCCAGAC AAGAAGTTTG AGACCGTGAA ACTGGCCTCT
    TGGTCCAAAG AAAAGCCAGG TGGCTGGTAT AGCACATTCC GTCGAGGGAA AAAGTTCTCC
    TACGTGGATG CCGATGGGGC CGCAGTGAGC GTGGTACAGC TGACCTTCCT GAAGTTGCTG
    AGTGCCACGG CCCGCCAGAG CTTCACATTC ACCTGCCAGA ACTCGGCCGC CTGGCTGGAC
    GAAGCTGCAG GTGACCACAG CCGCTCTGCC CGTTTCCTCG GGGCCAATGG GGAGGAGGTA
    TCCTTCAACC AGATGGCAGC CGCCACCATC ACAGTCCCCT ACGATGGCTG CAGGCTCCGG
    AAGGGACAGT CGAAGACCCT CTTTGAGTTC AGCTCCTCCC GAGTGGGATT TCTGCCCCTG
    TTGGACGTGG CAGCTGCTGA CTTTGGCCAG ACGAACCAGA AGTTTGGGTG GGTTCGCAGT
    GAAGGGACTG ATGTCAGACT ACAGAGGGAA GTGGGACTTC CCTGGACTGA GCTGCGTACT
    CTTTCTGCTG CCTCAGCCCA GCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805112.1
    CDS153..5459
    Translation

    Target ORF information:

    RefSeq Version XM_010806810.2
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type V alpha 3 (COL5A3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806810.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCGGGAC GCAGGCCGTA GATCCTGTGG ATGTGCTGAA GGCACTGGGC
    ATGCGGGGGG GCCAGGCTGG GATCCCTGAG GGACCCGGCC TCTGCCCCCA GAGGGTCCCG
    GAGGGCGACC GAGCATTCCG GGTGGGCAAG ACTGGCACAC TTGGTGTCCC CACGCAGGAG
    CTCTTCCAAG ATGGGCACTT TCCTGAGAAC TTCTCCATCC TGATCACCCT GCGGGGCCAG
    CCGGCCAACC GTTCTGTCCT TCTGTCCATC TATGATGAGG GCGGCGTCCG GCAGCTCGGC
    TTGGCCTTGG GGCCAGCTCT GGACCTCCTA GGTGACTCCT TCAGCCCCCT CCCCCAGCAG
    GTCAACCTCA CGGATGGCAG GTGGCACCGT GTGGCAGTCA GTGTGGACGG TGGGATGGTG
    ACCCTGGTGG CTGATTGTGA ACCTCAGCTC CCCATGTTGG CTCAGGGACC TCGATTCATC
    AGTACAGCGG GACTCACTGT GCTGGGGACC CAGGACCTTG GCGAGGAGAC TTTTGAGGGA
    GATGTTCAGG AGCTGGTGAT AAGCCCAGAT CCCCAGGCTG CCTTCCAGGC ATGTGAGCGG
    TACCTTCCTG GCTGTGATGA CCTGGATCCA ACAGTCACCA CGGTCCCTCA GGGGGAGCCA
    GAAACCCCTC CCCCTAGGCG GAAGGGGAAA GGAAAAGGGA AGAAAAAAGG GCGAGGTCGT
    AAAGGGAAGG GCAGGAAGAA GAAGAACAAG GAAACTGTGG CTGTGAGTCC TCCTCCTGGC
    TCCCTGGACA ACCAGACCTC CACTGACATC CCCAAGACAG AGACACCAGA ACCAACTCTG
    TCTCCGACAC CCACGCCTTT GGTCATCACC ACAACTGTGA CCTTTGGTAG CAATGTCACC
    ATCCTAGAGG AGGGCCTGGA CCCTGACAAT GGAACTGAAC TGGAGACCCT GGAGACTGAG
    TTAGACAGAG AAGATGAAGA AGAAGGTGAT CCCACCATGG GCCCCCACTT CCGGGCAGCA
    GAACAGTCAT GGAATACTGA GTTCCAGATC TTTCCTGGTG CTGGGGAGAA AGGAGAAAAA
    GGAGAACCAG CTGTGGTCGA ACAGGGACAG CAGTTTGAAG GACCCCCAGG AGCCCCGGGA
    CCCCAAGGGG TGGTTGGCCC CTCAGGCCCT CCTGGCCCCC CAGGATTCCC TGGAGACCGT
    GGTCTACCGG GCCCTGCTGG CCTCCCAGGC ATCCCTGGCA TGGATGGGAT CCGGGGTCTC
    CCAGGCACTG TGATCATGAT GCCGTTCCAG TTTGCAAGAG GCTCTCACAA AGGACCCCCA
    GTATCCTTCC AGCAGGCCCA GGCTCAGGCA GTCCTGCAAC AAGCTCAGCT GTCTATGAAA
    GGCCCTCCTG GCCCGGTGGG GCTCACCGGA CGCCCAGGTC CCGTGGGTCT TCCTGGGTAT
    CCAGGTCTGA AAGGAGAGAT GGGAGAAATG GGGCCGCAGG GTCCCCGAGG GCTTCAGGGA
    CCTCATGGGC CCCCTGGCCG AGAGGGAAAG ATGGGCCGCG CTGGAGCAGA TGGGGCTCGG
    GGCCTCCCCG GCGCTACGGG ACCTAAGGGT GATCGGGGCT TTGATGGCCT GCCAGGGCTG
    CCTGGTGAGA AGGGCCAAAG GGGTGACTTT GGTTATGTGG GGAAACCTGG TCCCCCAGGA
    GAGGATGGAC GGAAGGGAGC AGAGGGACCT CCGGGGCCCA CTGGCCAGGC AGGGGAGCCG
    GGCCCCCGAG GACTGATTGG CCCTAGAGGC TCCCCCGGAC CCCTGGGACG CCCGGGTGTG
    ATTGGAAGTG ATGGTGCTCC AGGTGCCAAA GGAAATGTGG GTCCTCCAGG AGAACCAGGC
    CCCCCAGGAC AGCAGGGAAA TCCTGGGTCC CAGGGAATTC CCGGCCCCCA GGGACCCATC
    GGCACTCCTG GGGAGAAGGG TCCTCCTGGA AACCCAGGAA TTCCAGGCCT CCCAGGATCT
    GAAGGCCCTC CGGGTCACCC AGGTCATGAG GGCCCAACAG GAGAGAAAGG GGCCCAGGGT
    CCAGCAGGGT CAGCAGGCCC TCCGGGCTAT CCAGGACCTC GGGGTGTGAA GGGTACCTCT
    GGCAACCGGG GCCTCCAGGG GGAGAAAGGA GAGAAGGGAG AGGATGGTTT CCCAGGCTTC
    AAGGGTGATG CGGGGCTTAA AGGAGATCGG GGGCAACCCG GACCTCCAGG TCCTCGGGGA
    GAGGATGGTC CGGAGGGGCT AAAGGGGCAG GAGGGGCTGC TTGGCGAGGA GGGTCCCCCA
    GGCTCAGCTG GGGAGAAGGG CAAGCTTGGC GTGCCGGGTC TCCCAGGTTA TCCAGGACGT
    CCAGGCCCTA AGGGATCTAT TGGCTTTCCG GGACCTCTGG GACCATTAGG AGAAAAAGGG
    AAGCGGGGGA AGGCTGGGCA GCCAGGCCTG GAAGGAGAGC GGGGACCACC AGGTGCCCGT
    GGAGAGAGAG GGCAACCAGG TGCCACAGGG CAGCCGGGCC CCAAGGGTGA TGTGGGCCAG
    GACGGAGCCC CTGGGTTCCC TGGAGAAAAG GGCCTCCCAG GTCTGCAAGG TCCTCCTGGG
    TTCTCTGGAC CAAAGGGTCC CCCTGGTCCC CAGGGAAAAG ATGGGGTACC TGGACACCCT
    GGACAGAGAG GAGAATTGGG TTTCCAAGGT CAGACAGGCC CACCTGGACC AGCTGGTGTC
    GTGGGTCCTC AGGGAAAGAC AGGAGAAGCA GGGCCTCTGG GTGAGAGGGG GCCCCCAGGG
    CCCCCTGGAC CTCCTGGTGA ACAAGGTCTT CCGGGCCTGG AAGGCAGAGA GGGGGCCAAG
    GGGGATGTGG GACCACTGGG ACCCCTTGGG AAGGAAGGGC CACCTGGACC CAGAGGTTTC
    CCTGGTCCTC ATGGAGCCCC CGGGGACCCA GGACCGACTG GTTTAAAGGG TGACAAGGGC
    CCCCCAGGTC CTGTTGGTGC CAACGGATCC CCTGGGGAGC GTGGTCCTGT AGGCCCAGCA
    GGGAGCATTG GGCTTCCTGG TCAAAGCGGA GGCCAAGGCC CCGTGGGCCC CACAGGCGAG
    AAGGGTTCCC CGGGTGAACG TGGCGCCCCT GGCCCAACTG GTAAAGATGG GATCCCCGGG
    CCCCTGGGGC TTCCTGGGCC CCCTGGAGCT GCGGGGCCTT CTGGCGAGGA AGGGGACAAG
    GGAGAAGTGG GTGCCCCCGG TCACAAAGGC AGCAAAGGTG ACAAGGGAGA TGCGGGCCCG
    CCTGGACCAA CAGGGATACG GGGTCTGGTG GGACACCCCG GCTCCCCGGG AGCAGACGGG
    GCTCAGGGAC GCCGGGGACC CCCAGGCCTC TTTGGGCAGA AAGGAGATGA TGGAGTGAGA
    GGCTTCCCTG GCATGATTGG TCCTCCTGGC CTACAGGGAA TGCCAGGCCC TGCTGGAGAA
    AAGGGAGAAG TTGGAGATGT AGGGTCCATG GGTCCCCACG GAGCTCCAGG CCCTCGGGGC
    CCCCAGGGCC CCAGCGGATC AGAGGGTCCT CCAGGGTTGC CTGGGGGAGT TGGTCAGCCT
    GGCGCTGTGG GGGAGAAGGG TGAGCCAGGA GAGCCTGGAG ACCCAGGACC CCCCGGGACC
    CCAGGCACCC CGGGCCCCAG GGGAGAAGTT GGTGAAAAGG GGGATACAGG CCCATCTGGG
    GCTGCTGGAC CCCCAGGCAA GAAAGGACCC CCTGGAGAGG ATGGAGCAAA GGGGAATGTG
    GGCCCCACAG GCCTCCCAGG AGACCTAGGC CCCCCCGGAG ACCCTGGAGT TTCGGGTCAA
    GATGGCCCCC CAGGGGAGAA GGGAAACCCT GGAGATGTGG GGGGACCGGG TCCACCTGGC
    GCTTCTGGGG AGCCTGGCGC CCCTGGGCCT CCTGGTAAAA GGGGTCCTTC TGGTCGCATG
    GGTCAAGAAG GTAGAGAAGG AGAGAAAGGT GCCAAGGGGG AACCAGGTCC TGATGGACCC
    CCAGGGAGGA CAGGCCCAGT AGGGGCTCGA GGGCCCCCTG GACGTGTTGG ACCTGAGGGT
    CTTCGTGGGA TCCCTGGACC TGTGGGCGAA CCAGGCCTCC TGGGACCTCC TGGACTGTTA
    GGCCCTCCTG GTCCCCTGGG GCCCTCTGGC CTACCAGGGC TGAAGGGAGA TGCTGGCCCC
    AAGGGAGAAA AGGGCCACAT TGGATTAATT GGTCTTGTCG GCCCCCCTGG GGAGGTTGGT
    GAGAAAGGGG ATCAGGGGTT GCCAGGAGTG CAAGGCTCCC CTGGCCTCCA GGGAGAACCG
    GGTTCTCCTG GCCCCATTGG CTCTCTGGGC CACCCTGGGC CCCAAGGCGT GGTGGGTCCT
    CCAGGACAGA AAGGCTCAAA GGGGTCCCCG GGATCCCAAG GTCCTCGTGG AGACGCTGGC
    CCCCCAGGTC CTCCTGGTCC CCCGGGTCCC TCTGCGGAGC TGCACGGGCT GCGCCGGCGC
    CGACGTTCCC TGCTAGGCTT GGGAACCCCG GAGGGCGGCC TGGAGGAGGT GCTGGCCTCG
    ATGGCGTCGC TGAGCTTCGA GTTAGAGCAG CTGCGGCGCC CTCCCGGCAC GGCTGAGCGC
    CCGGGCCTCG TGTGCAAAGA GTTGCACCGC AGCCATCCGC ATCTGCCAGA TGGGGAATAC
    TGGATTGATC CCAATCAAGG CTGCGCGCGG GATGCCCTCA GGGTTTTCTG TAACTTCACG
    GCAGGAGGAG AAACCTGCCT CTACCCAGAC AAGAAGTTTG AGACCGTGAA ACTGGCCTCT
    TGGTCCAAAG AAAAGCCAGG TGGCTGGTAT AGCACATTCC GTCGAGGGAA AAAGTTCTCC
    TACGTGGATG CCGATGGGGC CGCAGTGAGC GTGGTACAGC TGACCTTCCT GAAGTTGCTG
    AGTGCCACGG CCCGCCAGAG CTTCACATTC ACCTGCCAGA ACTCGGCCGC CTGGCTGGAC
    GAAGCTGCAG GTGACCACAG CCGCTCTGCC CGTTTCCTCG GGGCCAATGG GGAGGAGGTA
    TCCTTCAACC AGATGGCAGC CGCCACCATC ACAGTCCCCT ACGATGGCTG CAGGCTCCGG
    AAGGGACAGT CGAAGACCCT CTTTGAGTTC AGCTCCTCCC GAGTGGGATT TCTGCCCCTG
    TTGGACGTGG CAGCTGCTGA CTTTGGCCAG ACGAACCAGA AGTTTGGGTG GGTTCGCAGT
    GAAGGGACTG ATGTCAGACT ACAGAGGGAA GTGGGACTTC CCTGGACTGA GCTGCGTACT
    CTTTCTGCTG CCTCAGCCCA GCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46993
    Clone ID Related Accession (Same CDS sequence) XM_010806811.2
    Accession Version XM_010806811.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5304bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-3(V) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806811.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCGGGAC GCAGGCCGAT CCTGTGGATG TGCTGAAGGC ACTGGGCATG
    CGGGGGGGCC AGGCTGGGAT CCCTGAGGGA CCCGGCCTCT GCCCCCAGAG GGTCCCGGAG
    GGCGACCGAG CATTCCGGGT GGGCAAGACT GGCACACTTG GTGTCCCCAC GCAGGAGCTC
    TTCCAAGATG GGCACTTTCC TGAGAACTTC TCCATCCTGA TCACCCTGCG GGGCCAGCCG
    GCCAACCGTT CTGTCCTTCT GTCCATCTAT GATGAGGGCG GCGTCCGGCA GCTCGGCTTG
    GCCTTGGGGC CAGCTCTGGA CCTCCTAGGT GACTCCTTCA GCCCCCTCCC CCAGCAGGTC
    AACCTCACGG ATGGCAGGTG GCACCGTGTG GCAGTCAGTG TGGACGGTGG GATGGTGACC
    CTGGTGGCTG ATTGTGAACC TCAGCTCCCC ATGTTGGCTC AGGGACCTCG ATTCATCAGT
    ACAGCGGGAC TCACTGTGCT GGGGACCCAG GACCTTGGCG AGGAGACTTT TGAGGGAGAT
    GTTCAGGAGC TGGTGATAAG CCCAGATCCC CAGGCTGCCT TCCAGGCATG TGAGCGGTAC
    CTTCCTGGCT GTGATGACCT GGATCCAACA GTCACCACGG TCCCTCAGGG GGAGCCAGAA
    ACCCCTCCCC CTAGGCGGAA GGGGAAAGGA AAAGGGAAGA AAAAAGGGCG AGGTCGTAAA
    GGGAAGGGCA GGAAGAAGAA GAACAAGGAA ACTGTGGCTG TGAGTCCTCC TCCTGGCTCC
    CTGGACAACC AGACCTCCAC TGACATCCCC AAGACAGAGA CACCAGAACC AACTCTGTCT
    CCGACACCCA CGCCTTTGGT CATCACCACA ACTGTGACCT TTGGTAGCAA TGTCACCATC
    CTAGAGGAGG GCCTGGACCC TGACAATGGA ACTGAACTGG AGACCCTGGA GACTGAGTTA
    GACAGAGAAG ATGAAGAAGA AGGTGATCCC ACCATGGGCC CCCACTTCCG GGCAGCAGAA
    CAGTCATGGA ATACTGAGTT CCAGATCTTT CCTGGTGCTG GGGAGAAAGG AGAAAAAGGA
    GAACCAGCTG TGGTCGAACA GGGACAGCAG TTTGAAGGAC CCCCAGGAGC CCCGGGACCC
    CAAGGGGTGG TTGGCCCCTC AGGCCCTCCT GGCCCCCCAG GATTCCCTGG AGACCGTGGT
    CTACCGGGCC CTGCTGGCCT CCCAGGCATC CCTGGCATGG ATGGGATCCG GGGTCTCCCA
    GGCACTGTGA TCATGATGCC GTTCCAGTTT GCAAGAGGCT CTCACAAAGG ACCCCCAGTA
    TCCTTCCAGC AGGCCCAGGC TCAGGCAGTC CTGCAACAAG CTCAGCTGTC TATGAAAGGC
    CCTCCTGGCC CGGTGGGGCT CACCGGACGC CCAGGTCCCG TGGGTCTTCC TGGGTATCCA
    GGTCTGAAAG GAGAGATGGG AGAAATGGGG CCGCAGGGTC CCCGAGGGCT TCAGGGACCT
    CATGGGCCCC CTGGCCGAGA GGGAAAGATG GGCCGCGCTG GAGCAGATGG GGCTCGGGGC
    CTCCCCGGCG CTACGGGACC TAAGGGTGAT CGGGGCTTTG ATGGCCTGCC AGGGCTGCCT
    GGTGAGAAGG GCCAAAGGGG TGACTTTGGT TATGTGGGGA AACCTGGTCC CCCAGGAGAG
    GATGGACGGA AGGGAGCAGA GGGACCTCCG GGGCCCACTG GCCAGGCAGG GGAGCCGGGC
    CCCCGAGGAC TGATTGGCCC TAGAGGCTCC CCCGGACCCC TGGGACGCCC GGGTGTGATT
    GGAAGTGATG GTGCTCCAGG TGCCAAAGGA AATGTGGGTC CTCCAGGAGA ACCAGGCCCC
    CCAGGACAGC AGGGAAATCC TGGGTCCCAG GGAATTCCCG GCCCCCAGGG ACCCATCGGC
    ACTCCTGGGG AGAAGGGTCC TCCTGGAAAC CCAGGAATTC CAGGCCTCCC AGGATCTGAA
    GGCCCTCCGG GTCACCCAGG TCATGAGGGC CCAACAGGAG AGAAAGGGGC CCAGGGTCCA
    GCAGGGTCAG CAGGCCCTCC GGGCTATCCA GGACCTCGGG GTGTGAAGGG TACCTCTGGC
    AACCGGGGCC TCCAGGGGGA GAAAGGAGAG AAGGGAGAGG ATGGTTTCCC AGGCTTCAAG
    GGTGATGCGG GGCTTAAAGG AGATCGGGGG CAACCCGGAC CTCCAGGTCC TCGGGGAGAG
    GATGGTCCGG AGGGGCTAAA GGGGCAGGAG GGGCTGCTTG GCGAGGAGGG TCCCCCAGGC
    TCAGCTGGGG AGAAGGGCAA GCTTGGCGTG CCGGGTCTCC CAGGTTATCC AGGACGTCCA
    GGCCCTAAGG GATCTATTGG CTTTCCGGGA CCTCTGGGAC CATTAGGAGA AAAAGGGAAG
    CGGGGGAAGG CTGGGCAGCC AGGCCTGGAA GGAGAGCGGG GACCACCAGG TGCCCGTGGA
    GAGAGAGGGC AACCAGGTGC CACAGGGCAG CCGGGCCCCA AGGGTGATGT GGGCCAGGAC
    GGAGCCCCTG GGTTCCCTGG AGAAAAGGGC CTCCCAGGTC TGCAAGGTCC TCCTGGGTTC
    TCTGGACCAA AGGGTCCCCC TGGTCCCCAG GGAAAAGATG GGGTACCTGG ACACCCTGGA
    CAGAGAGGAG AATTGGGTTT CCAAGGTCAG ACAGGCCCAC CTGGACCAGC TGGTGTCGTG
    GGTCCTCAGG GAAAGACAGG AGAAGCAGGG CCTCTGGGTG AGAGGGGGCC CCCAGGGCCC
    CCTGGACCTC CTGGTGAACA AGGTCTTCCG GGCCTGGAAG GCAGAGAGGG GGCCAAGGGG
    GATGTGGGAC CACTGGGACC CCTTGGGAAG GAAGGGCCAC CTGGACCCAG AGGTTTCCCT
    GGTCCTCATG GAGCCCCCGG GGACCCAGGA CCGACTGGTT TAAAGGGTGA CAAGGGCCCC
    CCAGGTCCTG TTGGTGCCAA CGGATCCCCT GGGGAGCGTG GTCCTGTAGG CCCAGCAGGG
    AGCATTGGGC TTCCTGGTCA AAGCGGAGGC CAAGGCCCCG TGGGCCCCAC AGGCGAGAAG
    GGTTCCCCGG GTGAACGTGG CGCCCCTGGC CCAACTGGTA AAGATGGGAT CCCCGGGCCC
    CTGGGGCTTC CTGGGCCCCC TGGAGCTGCG GGGCCTTCTG GCGAGGAAGG GGACAAGGGA
    GAAGTGGGTG CCCCCGGTCA CAAAGGCAGC AAAGGTGACA AGGGAGATGC GGGCCCGCCT
    GGACCAACAG GGATACGGGG TCTGGTGGGA CACCCCGGCT CCCCGGGAGC AGACGGGGCT
    CAGGGACGCC GGGGACCCCC AGGCCTCTTT GGGCAGAAAG GAGATGATGG AGTGAGAGGC
    TTCCCTGGCA TGATTGGTCC TCCTGGCCTA CAGGGAATGC CAGGCCCTGC TGGAGAAAAG
    GGAGAAGTTG GAGATGTAGG GTCCATGGGT CCCCACGGAG CTCCAGGCCC TCGGGGCCCC
    CAGGGCCCCA GCGGATCAGA GGGTCCTCCA GGGTTGCCTG GGGGAGTTGG TCAGCCTGGC
    GCTGTGGGGG AGAAGGGTGA GCCAGGAGAG CCTGGAGACC CAGGACCCCC CGGGACCCCA
    GGCACCCCGG GCCCCAGGGG AGAAGTTGGT GAAAAGGGGG ATACAGGCCC ATCTGGGGCT
    GCTGGACCCC CAGGCAAGAA AGGACCCCCT GGAGAGGATG GAGCAAAGGG GAATGTGGGC
    CCCACAGGCC TCCCAGGAGA CCTAGGCCCC CCCGGAGACC CTGGAGTTTC GGGTCAAGAT
    GGCCCCCCAG GGGAGAAGGG AAACCCTGGA GATGTGGGGG GACCGGGTCC ACCTGGCGCT
    TCTGGGGAGC CTGGCGCCCC TGGGCCTCCT GGTAAAAGGG GTCCTTCTGG TCGCATGGGT
    CAAGAAGGTA GAGAAGGAGA GAAAGGTGCC AAGGGGGAAC CAGGTCCTGA TGGACCCCCA
    GGGAGGACAG GCCCAGTAGG GGCTCGAGGG CCCCCTGGAC GTGTTGGACC TGAGGGTCTT
    CGTGGGATCC CTGGACCTGT GGGCGAACCA GGCCTCCTGG GACCTCCTGG ACTGTTAGGC
    CCTCCTGGTC CCCTGGGGCC CTCTGGCCTA CCAGGGCTGA AGGGAGATGC TGGCCCCAAG
    GGAGAAAAGG GCCACATTGG ATTAATTGGT CTTGTCGGCC CCCCTGGGGA GGTTGGTGAG
    AAAGGGGATC AGGGGTTGCC AGGAGTGCAA GGCTCCCCTG GCCTCCAGGG AGAACCGGGT
    TCTCCTGGCC CCATTGGCTC TCTGGGCCAC CCTGGGCCCC AAGGCGTGGT GGGTCCTCCA
    GGACAGAAAG GCTCAAAGGG GTCCCCGGGA TCCCAAGGTC CTCGTGGAGA CGCTGGCCCC
    CCAGGTCCTC CTGGTCCCCC GGGTCCCTCT GCGGAGCTGC ACGGGCTGCG CCGGCGCCGA
    CGTTCCCTGC TAGGCTTGGG AACCCCGGAG GGCGGCCTGG AGGAGGTGCT GGCCTCGATG
    GCGTCGCTGA GCTTCGAGTT AGAGCAGCTG CGGCGCCCTC CCGGCACGGC TGAGCGCCCG
    GGCCTCGTGT GCAAAGAGTT GCACCGCAGC CATCCGCATC TGCCAGATGG GGAATACTGG
    ATTGATCCCA ATCAAGGCTG CGCGCGGGAT GCCCTCAGGG TTTTCTGTAA CTTCACGGCA
    GGAGGAGAAA CCTGCCTCTA CCCAGACAAG AAGTTTGAGA CCGTGAAACT GGCCTCTTGG
    TCCAAAGAAA AGCCAGGTGG CTGGTATAGC ACATTCCGTC GAGGGAAAAA GTTCTCCTAC
    GTGGATGCCG ATGGGGCCGC AGTGAGCGTG GTACAGCTGA CCTTCCTGAA GTTGCTGAGT
    GCCACGGCCC GCCAGAGCTT CACATTCACC TGCCAGAACT CGGCCGCCTG GCTGGACGAA
    GCTGCAGGTG ACCACAGCCG CTCTGCCCGT TTCCTCGGGG CCAATGGGGA GGAGGTATCC
    TTCAACCAGA TGGCAGCCGC CACCATCACA GTCCCCTACG ATGGCTGCAG GCTCCGGAAG
    GGACAGTCGA AGACCCTCTT TGAGTTCAGC TCCTCCCGAG TGGGATTTCT GCCCCTGTTG
    GACGTGGCAG CTGCTGACTT TGGCCAGACG AACCAGAAGT TTGGGTGGGT TCGCAGTGAA
    GGGACTGATG TCAGACTACA GAGGGAAGTG GGACTTCCCT GGACTGAGCT GCGTACTCTT
    TCTGCTGCCT CAGCCCAGCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805113.1
    CDS153..5456
    Translation

    Target ORF information:

    RefSeq Version XM_010806811.2
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type V alpha 3 (COL5A3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806811.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCGGGAC GCAGGCCGAT CCTGTGGATG TGCTGAAGGC ACTGGGCATG
    CGGGGGGGCC AGGCTGGGAT CCCTGAGGGA CCCGGCCTCT GCCCCCAGAG GGTCCCGGAG
    GGCGACCGAG CATTCCGGGT GGGCAAGACT GGCACACTTG GTGTCCCCAC GCAGGAGCTC
    TTCCAAGATG GGCACTTTCC TGAGAACTTC TCCATCCTGA TCACCCTGCG GGGCCAGCCG
    GCCAACCGTT CTGTCCTTCT GTCCATCTAT GATGAGGGCG GCGTCCGGCA GCTCGGCTTG
    GCCTTGGGGC CAGCTCTGGA CCTCCTAGGT GACTCCTTCA GCCCCCTCCC CCAGCAGGTC
    AACCTCACGG ATGGCAGGTG GCACCGTGTG GCAGTCAGTG TGGACGGTGG GATGGTGACC
    CTGGTGGCTG ATTGTGAACC TCAGCTCCCC ATGTTGGCTC AGGGACCTCG ATTCATCAGT
    ACAGCGGGAC TCACTGTGCT GGGGACCCAG GACCTTGGCG AGGAGACTTT TGAGGGAGAT
    GTTCAGGAGC TGGTGATAAG CCCAGATCCC CAGGCTGCCT TCCAGGCATG TGAGCGGTAC
    CTTCCTGGCT GTGATGACCT GGATCCAACA GTCACCACGG TCCCTCAGGG GGAGCCAGAA
    ACCCCTCCCC CTAGGCGGAA GGGGAAAGGA AAAGGGAAGA AAAAAGGGCG AGGTCGTAAA
    GGGAAGGGCA GGAAGAAGAA GAACAAGGAA ACTGTGGCTG TGAGTCCTCC TCCTGGCTCC
    CTGGACAACC AGACCTCCAC TGACATCCCC AAGACAGAGA CACCAGAACC AACTCTGTCT
    CCGACACCCA CGCCTTTGGT CATCACCACA ACTGTGACCT TTGGTAGCAA TGTCACCATC
    CTAGAGGAGG GCCTGGACCC TGACAATGGA ACTGAACTGG AGACCCTGGA GACTGAGTTA
    GACAGAGAAG ATGAAGAAGA AGGTGATCCC ACCATGGGCC CCCACTTCCG GGCAGCAGAA
    CAGTCATGGA ATACTGAGTT CCAGATCTTT CCTGGTGCTG GGGAGAAAGG AGAAAAAGGA
    GAACCAGCTG TGGTCGAACA GGGACAGCAG TTTGAAGGAC CCCCAGGAGC CCCGGGACCC
    CAAGGGGTGG TTGGCCCCTC AGGCCCTCCT GGCCCCCCAG GATTCCCTGG AGACCGTGGT
    CTACCGGGCC CTGCTGGCCT CCCAGGCATC CCTGGCATGG ATGGGATCCG GGGTCTCCCA
    GGCACTGTGA TCATGATGCC GTTCCAGTTT GCAAGAGGCT CTCACAAAGG ACCCCCAGTA
    TCCTTCCAGC AGGCCCAGGC TCAGGCAGTC CTGCAACAAG CTCAGCTGTC TATGAAAGGC
    CCTCCTGGCC CGGTGGGGCT CACCGGACGC CCAGGTCCCG TGGGTCTTCC TGGGTATCCA
    GGTCTGAAAG GAGAGATGGG AGAAATGGGG CCGCAGGGTC CCCGAGGGCT TCAGGGACCT
    CATGGGCCCC CTGGCCGAGA GGGAAAGATG GGCCGCGCTG GAGCAGATGG GGCTCGGGGC
    CTCCCCGGCG CTACGGGACC TAAGGGTGAT CGGGGCTTTG ATGGCCTGCC AGGGCTGCCT
    GGTGAGAAGG GCCAAAGGGG TGACTTTGGT TATGTGGGGA AACCTGGTCC CCCAGGAGAG
    GATGGACGGA AGGGAGCAGA GGGACCTCCG GGGCCCACTG GCCAGGCAGG GGAGCCGGGC
    CCCCGAGGAC TGATTGGCCC TAGAGGCTCC CCCGGACCCC TGGGACGCCC GGGTGTGATT
    GGAAGTGATG GTGCTCCAGG TGCCAAAGGA AATGTGGGTC CTCCAGGAGA ACCAGGCCCC
    CCAGGACAGC AGGGAAATCC TGGGTCCCAG GGAATTCCCG GCCCCCAGGG ACCCATCGGC
    ACTCCTGGGG AGAAGGGTCC TCCTGGAAAC CCAGGAATTC CAGGCCTCCC AGGATCTGAA
    GGCCCTCCGG GTCACCCAGG TCATGAGGGC CCAACAGGAG AGAAAGGGGC CCAGGGTCCA
    GCAGGGTCAG CAGGCCCTCC GGGCTATCCA GGACCTCGGG GTGTGAAGGG TACCTCTGGC
    AACCGGGGCC TCCAGGGGGA GAAAGGAGAG AAGGGAGAGG ATGGTTTCCC AGGCTTCAAG
    GGTGATGCGG GGCTTAAAGG AGATCGGGGG CAACCCGGAC CTCCAGGTCC TCGGGGAGAG
    GATGGTCCGG AGGGGCTAAA GGGGCAGGAG GGGCTGCTTG GCGAGGAGGG TCCCCCAGGC
    TCAGCTGGGG AGAAGGGCAA GCTTGGCGTG CCGGGTCTCC CAGGTTATCC AGGACGTCCA
    GGCCCTAAGG GATCTATTGG CTTTCCGGGA CCTCTGGGAC CATTAGGAGA AAAAGGGAAG
    CGGGGGAAGG CTGGGCAGCC AGGCCTGGAA GGAGAGCGGG GACCACCAGG TGCCCGTGGA
    GAGAGAGGGC AACCAGGTGC CACAGGGCAG CCGGGCCCCA AGGGTGATGT GGGCCAGGAC
    GGAGCCCCTG GGTTCCCTGG AGAAAAGGGC CTCCCAGGTC TGCAAGGTCC TCCTGGGTTC
    TCTGGACCAA AGGGTCCCCC TGGTCCCCAG GGAAAAGATG GGGTACCTGG ACACCCTGGA
    CAGAGAGGAG AATTGGGTTT CCAAGGTCAG ACAGGCCCAC CTGGACCAGC TGGTGTCGTG
    GGTCCTCAGG GAAAGACAGG AGAAGCAGGG CCTCTGGGTG AGAGGGGGCC CCCAGGGCCC
    CCTGGACCTC CTGGTGAACA AGGTCTTCCG GGCCTGGAAG GCAGAGAGGG GGCCAAGGGG
    GATGTGGGAC CACTGGGACC CCTTGGGAAG GAAGGGCCAC CTGGACCCAG AGGTTTCCCT
    GGTCCTCATG GAGCCCCCGG GGACCCAGGA CCGACTGGTT TAAAGGGTGA CAAGGGCCCC
    CCAGGTCCTG TTGGTGCCAA CGGATCCCCT GGGGAGCGTG GTCCTGTAGG CCCAGCAGGG
    AGCATTGGGC TTCCTGGTCA AAGCGGAGGC CAAGGCCCCG TGGGCCCCAC AGGCGAGAAG
    GGTTCCCCGG GTGAACGTGG CGCCCCTGGC CCAACTGGTA AAGATGGGAT CCCCGGGCCC
    CTGGGGCTTC CTGGGCCCCC TGGAGCTGCG GGGCCTTCTG GCGAGGAAGG GGACAAGGGA
    GAAGTGGGTG CCCCCGGTCA CAAAGGCAGC AAAGGTGACA AGGGAGATGC GGGCCCGCCT
    GGACCAACAG GGATACGGGG TCTGGTGGGA CACCCCGGCT CCCCGGGAGC AGACGGGGCT
    CAGGGACGCC GGGGACCCCC AGGCCTCTTT GGGCAGAAAG GAGATGATGG AGTGAGAGGC
    TTCCCTGGCA TGATTGGTCC TCCTGGCCTA CAGGGAATGC CAGGCCCTGC TGGAGAAAAG
    GGAGAAGTTG GAGATGTAGG GTCCATGGGT CCCCACGGAG CTCCAGGCCC TCGGGGCCCC
    CAGGGCCCCA GCGGATCAGA GGGTCCTCCA GGGTTGCCTG GGGGAGTTGG TCAGCCTGGC
    GCTGTGGGGG AGAAGGGTGA GCCAGGAGAG CCTGGAGACC CAGGACCCCC CGGGACCCCA
    GGCACCCCGG GCCCCAGGGG AGAAGTTGGT GAAAAGGGGG ATACAGGCCC ATCTGGGGCT
    GCTGGACCCC CAGGCAAGAA AGGACCCCCT GGAGAGGATG GAGCAAAGGG GAATGTGGGC
    CCCACAGGCC TCCCAGGAGA CCTAGGCCCC CCCGGAGACC CTGGAGTTTC GGGTCAAGAT
    GGCCCCCCAG GGGAGAAGGG AAACCCTGGA GATGTGGGGG GACCGGGTCC ACCTGGCGCT
    TCTGGGGAGC CTGGCGCCCC TGGGCCTCCT GGTAAAAGGG GTCCTTCTGG TCGCATGGGT
    CAAGAAGGTA GAGAAGGAGA GAAAGGTGCC AAGGGGGAAC CAGGTCCTGA TGGACCCCCA
    GGGAGGACAG GCCCAGTAGG GGCTCGAGGG CCCCCTGGAC GTGTTGGACC TGAGGGTCTT
    CGTGGGATCC CTGGACCTGT GGGCGAACCA GGCCTCCTGG GACCTCCTGG ACTGTTAGGC
    CCTCCTGGTC CCCTGGGGCC CTCTGGCCTA CCAGGGCTGA AGGGAGATGC TGGCCCCAAG
    GGAGAAAAGG GCCACATTGG ATTAATTGGT CTTGTCGGCC CCCCTGGGGA GGTTGGTGAG
    AAAGGGGATC AGGGGTTGCC AGGAGTGCAA GGCTCCCCTG GCCTCCAGGG AGAACCGGGT
    TCTCCTGGCC CCATTGGCTC TCTGGGCCAC CCTGGGCCCC AAGGCGTGGT GGGTCCTCCA
    GGACAGAAAG GCTCAAAGGG GTCCCCGGGA TCCCAAGGTC CTCGTGGAGA CGCTGGCCCC
    CCAGGTCCTC CTGGTCCCCC GGGTCCCTCT GCGGAGCTGC ACGGGCTGCG CCGGCGCCGA
    CGTTCCCTGC TAGGCTTGGG AACCCCGGAG GGCGGCCTGG AGGAGGTGCT GGCCTCGATG
    GCGTCGCTGA GCTTCGAGTT AGAGCAGCTG CGGCGCCCTC CCGGCACGGC TGAGCGCCCG
    GGCCTCGTGT GCAAAGAGTT GCACCGCAGC CATCCGCATC TGCCAGATGG GGAATACTGG
    ATTGATCCCA ATCAAGGCTG CGCGCGGGAT GCCCTCAGGG TTTTCTGTAA CTTCACGGCA
    GGAGGAGAAA CCTGCCTCTA CCCAGACAAG AAGTTTGAGA CCGTGAAACT GGCCTCTTGG
    TCCAAAGAAA AGCCAGGTGG CTGGTATAGC ACATTCCGTC GAGGGAAAAA GTTCTCCTAC
    GTGGATGCCG ATGGGGCCGC AGTGAGCGTG GTACAGCTGA CCTTCCTGAA GTTGCTGAGT
    GCCACGGCCC GCCAGAGCTT CACATTCACC TGCCAGAACT CGGCCGCCTG GCTGGACGAA
    GCTGCAGGTG ACCACAGCCG CTCTGCCCGT TTCCTCGGGG CCAATGGGGA GGAGGTATCC
    TTCAACCAGA TGGCAGCCGC CACCATCACA GTCCCCTACG ATGGCTGCAG GCTCCGGAAG
    GGACAGTCGA AGACCCTCTT TGAGTTCAGC TCCTCCCGAG TGGGATTTCT GCCCCTGTTG
    GACGTGGCAG CTGCTGACTT TGGCCAGACG AACCAGAAGT TTGGGTGGGT TCGCAGTGAA
    GGGACTGATG TCAGACTACA GAGGGAAGTG GGACTTCCCT GGACTGAGCT GCGTACTCTT
    TCTGCTGCCT CAGCCCAGCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46994
    Clone ID Related Accession (Same CDS sequence) XM_010806813.2
    Accession Version XM_010806813.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5244bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-3(V) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806813.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCGGGAC GCAGGCCGTA GATCCTGTGG ATGTGCTGAA GGCACTGGGC
    ATGCGGGGGG GCCAGGCTGG GATCCCTGAG GGACCCGGCC TCTGCCCCCA GAGGGTCCCG
    GAGGGCGACC GAGCATTCCG GGTGGGCAAG ACTGGCACAC TTGGTGTCCC CACGCAGGAG
    CTCTTCCAAG ATGGGCACTT TCCTGAGAAC TTCTCCATCC TGATCACCCT GCGGGGCCAG
    CCGGCCAACC GTTCTGTCCT TCTGTCCATC TATGATGAGG GCGGCGTCCG GCAGCTCGGC
    TTGGCCTTGG GGCCAGCTCT GGACCTCCTA GGTGACTCCT TCAGCCCCCT CCCCCAGCAG
    GTCAACCTCA CGGATGGCAG GTGGCACCGT GTGGCAGTCA GTGTGGACGG TGGGATGGTG
    ACCCTGGTGG CTGATTGTGA ACCTCAGCTC CCCATGTTGG CTCAGGGACC TCGATTCATC
    AGTACAGCGG GACTCACTGT GCTGGGGACC CAGGACCTTG GCGAGGAGAC TTTTGAGGGA
    GATGTTCAGG AGCTGGTGAT AAGCCCAGAT CCCCAGGCTG CCTTCCAGGC ATGTGAGCGG
    TACCTTCCTG GCTGTGATGA CCTGGATCCA ACAGTCACCA CGGTCCCTCA GGGGGAGCCA
    GAAACCCCTC CCCCTAGGCG GAAGGGGAAA GGAAAAGGGA AGAAAAAAGG GCGAGGTCGT
    AAAGGGAAGG GCAGGAAGAA GAAGAACAAG GAAACTGTGG CTGTGAGTCC TCCTCCTGGC
    TCCCTGGACA ACCAGACCTC CACTGACATC CCCAAGACAG AGACACCAGA ACCAACTCTG
    TCTCCGACAC CCACGCCTTT GGTCATCACC ACAACTGTGA CCTTTGGTAG CAATGTCACC
    ATCCTAGAGG AGGGCCTGGA CCCTGACAAT GGAACTGAAC TGGAGACCCT GGAGACTGAG
    TTAGACAGAG AAGATGAAGA AGAAGGTGAT CCCACCATGG GCCCCCACTT CCGGGCAGCA
    GAACAGTCAT GGAATACTGA GTTCCAGATC TTTCCTGGTG CTGGGGAGAA AGGAGAAAAA
    GGAGAACCAG CTGTGGTCGA ACAGGGACAG CAGTTTGAAG GACCCCCAGG AGCCCCGGGA
    CCCCAAGGCC CTGCTGGCCT CCCAGGCATC CCTGGCATGG ATGGGATCCG GGGTCTCCCA
    GGCACTGTGA TCATGATGCC GTTCCAGTTT GCAAGAGGCT CTCACAAAGG ACCCCCAGTA
    TCCTTCCAGC AGGCCCAGGC TCAGGCAGTC CTGCAACAAG CTCAGCTGTC TATGAAAGGC
    CCTCCTGGCC CGGTGGGGCT CACCGGACGC CCAGGTCCCG TGGGTCTTCC TGGGTATCCA
    GGTCTGAAAG GAGAGATGGG AGAAATGGGG CCGCAGGGTC CCCGAGGGCT TCAGGGACCT
    CATGGGCCCC CTGGCCGAGA GGGAAAGATG GGCCGCGCTG GAGCAGATGG GGCTCGGGGC
    CTCCCCGGCG CTACGGGACC TAAGGGTGAT CGGGGCTTTG ATGGCCTGCC AGGGCTGCCT
    GGTGAGAAGG GCCAAAGGGG TGACTTTGGT TATGTGGGGA AACCTGGTCC CCCAGGAGAG
    GATGGACGGA AGGGAGCAGA GGGACCTCCG GGGCCCACTG GCCAGGCAGG GGAGCCGGGC
    CCCCGAGGAC TGATTGGCCC TAGAGGCTCC CCCGGACCCC TGGGACGCCC GGGTGTGATT
    GGAAGTGATG GTGCTCCAGG TGCCAAAGGA AATGTGGGTC CTCCAGGAGA ACCAGGCCCC
    CCAGGACAGC AGGGAAATCC TGGGTCCCAG GGAATTCCCG GCCCCCAGGG ACCCATCGGC
    ACTCCTGGGG AGAAGGGTCC TCCTGGAAAC CCAGGAATTC CAGGCCTCCC AGGATCTGAA
    GGCCCTCCGG GTCACCCAGG TCATGAGGGC CCAACAGGAG AGAAAGGGGC CCAGGGTCCA
    GCAGGGTCAG CAGGCCCTCC GGGCTATCCA GGACCTCGGG GTGTGAAGGG TACCTCTGGC
    AACCGGGGCC TCCAGGGGGA GAAAGGAGAG AAGGGAGAGG ATGGTTTCCC AGGCTTCAAG
    GGTGATGCGG GGCTTAAAGG AGATCGGGGG CAACCCGGAC CTCCAGGTCC TCGGGGAGAG
    GATGGTCCGG AGGGGCTAAA GGGGCAGGAG GGGCTGCTTG GCGAGGAGGG TCCCCCAGGC
    TCAGCTGGGG AGAAGGGCAA GCTTGGCGTG CCGGGTCTCC CAGGTTATCC AGGACGTCCA
    GGCCCTAAGG GATCTATTGG CTTTCCGGGA CCTCTGGGAC CATTAGGAGA AAAAGGGAAG
    CGGGGGAAGG CTGGGCAGCC AGGCCTGGAA GGAGAGCGGG GACCACCAGG TGCCCGTGGA
    GAGAGAGGGC AACCAGGTGC CACAGGGCAG CCGGGCCCCA AGGGTGATGT GGGCCAGGAC
    GGAGCCCCTG GGTTCCCTGG AGAAAAGGGC CTCCCAGGTC TGCAAGGTCC TCCTGGGTTC
    TCTGGACCAA AGGGTCCCCC TGGTCCCCAG GGAAAAGATG GGGTACCTGG ACACCCTGGA
    CAGAGAGGAG AATTGGGTTT CCAAGGTCAG ACAGGCCCAC CTGGACCAGC TGGTGTCGTG
    GGTCCTCAGG GAAAGACAGG AGAAGCAGGG CCTCTGGGTG AGAGGGGGCC CCCAGGGCCC
    CCTGGACCTC CTGGTGAACA AGGTCTTCCG GGCCTGGAAG GCAGAGAGGG GGCCAAGGGG
    GATGTGGGAC CACTGGGACC CCTTGGGAAG GAAGGGCCAC CTGGACCCAG AGGTTTCCCT
    GGTCCTCATG GAGCCCCCGG GGACCCAGGA CCGACTGGTT TAAAGGGTGA CAAGGGCCCC
    CCAGGTCCTG TTGGTGCCAA CGGATCCCCT GGGGAGCGTG GTCCTGTAGG CCCAGCAGGG
    AGCATTGGGC TTCCTGGTCA AAGCGGAGGC CAAGGCCCCG TGGGCCCCAC AGGCGAGAAG
    GGTTCCCCGG GTGAACGTGG CGCCCCTGGC CCAACTGGTA AAGATGGGAT CCCCGGGCCC
    CTGGGGCTTC CTGGGCCCCC TGGAGCTGCG GGGCCTTCTG GCGAGGAAGG GGACAAGGGA
    GAAGTGGGTG CCCCCGGTCA CAAAGGCAGC AAAGGTGACA AGGGAGATGC GGGCCCGCCT
    GGACCAACAG GGATACGGGG TCTGGTGGGA CACCCCGGCT CCCCGGGAGC AGACGGGGCT
    CAGGGACGCC GGGGACCCCC AGGCCTCTTT GGGCAGAAAG GAGATGATGG AGTGAGAGGC
    TTCCCTGGCA TGATTGGTCC TCCTGGCCTA CAGGGAATGC CAGGCCCTGC TGGAGAAAAG
    GGAGAAGTTG GAGATGTAGG GTCCATGGGT CCCCACGGAG CTCCAGGCCC TCGGGGCCCC
    CAGGGCCCCA GCGGATCAGA GGGTCCTCCA GGGTTGCCTG GGGGAGTTGG TCAGCCTGGC
    GCTGTGGGGG AGAAGGGTGA GCCAGGAGAG CCTGGAGACC CAGGACCCCC CGGGACCCCA
    GGCACCCCGG GCCCCAGGGG AGAAGTTGGT GAAAAGGGGG ATACAGGCCC ATCTGGGGCT
    GCTGGACCCC CAGGCAAGAA AGGACCCCCT GGAGAGGATG GAGCAAAGGG GAATGTGGGC
    CCCACAGGCC TCCCAGGAGA CCTAGGCCCC CCCGGAGACC CTGGAGTTTC GGGTCAAGAT
    GGCCCCCCAG GGGAGAAGGG AAACCCTGGA GATGTGGGGG GACCGGGTCC ACCTGGCGCT
    TCTGGGGAGC CTGGCGCCCC TGGGCCTCCT GGTAAAAGGG GTCCTTCTGG TCGCATGGGT
    CAAGAAGGTA GAGAAGGAGA GAAAGGTGCC AAGGGGGAAC CAGGTCCTGA TGGACCCCCA
    GGGAGGACAG GCCCAGTAGG GGCTCGAGGG CCCCCTGGAC GTGTTGGACC TGAGGGTCTT
    CGTGGGATCC CTGGACCTGT GGGCGAACCA GGCCTCCTGG GACCTCCTGG ACTGTTAGGC
    CCTCCTGGTC CCCTGGGGCC CTCTGGCCTA CCAGGGCTGA AGGGAGATGC TGGCCCCAAG
    GGAGAAAAGG GCCACATTGG ATTAATTGGT CTTGTCGGCC CCCCTGGGGA GGTTGGTGAG
    AAAGGGGATC AGGGGTTGCC AGGAGTGCAA GGCTCCCCTG GCCTCCAGGG AGAACCGGGT
    TCTCCTGGCC CCATTGGCTC TCTGGGCCAC CCTGGGCCCC AAGGCGTGGT GGGTCCTCCA
    GGACAGAAAG GCTCAAAGGG GTCCCCGGGA TCCCAAGGTC CTCGTGGAGA CGCTGGCCCC
    CCAGGTCCTC CTGGTCCCCC GGGTCCCTCT GCGGAGCTGC ACGGGCTGCG CCGGCGCCGA
    CGTTCCCTGC TAGGCTTGGG AACCCCGGAG GGCGGCCTGG AGGAGGTGCT GGCCTCGATG
    GCGTCGCTGA GCTTCGAGTT AGAGCAGCTG CGGCGCCCTC CCGGCACGGC TGAGCGCCCG
    GGCCTCGTGT GCAAAGAGTT GCACCGCAGC CATCCGCATC TGCCAGATGG GGAATACTGG
    ATTGATCCCA ATCAAGGCTG CGCGCGGGAT GCCCTCAGGG TTTTCTGTAA CTTCACGGCA
    GGAGGAGAAA CCTGCCTCTA CCCAGACAAG AAGTTTGAGA CCGTGAAACT GGCCTCTTGG
    TCCAAAGAAA AGCCAGGTGG CTGGTATAGC ACATTCCGTC GAGGGAAAAA GTTCTCCTAC
    GTGGATGCCG ATGGGGCCGC AGTGAGCGTG GTACAGCTGA CCTTCCTGAA GTTGCTGAGT
    GCCACGGCCC GCCAGAGCTT CACATTCACC TGCCAGAACT CGGCCGCCTG GCTGGACGAA
    GCTGCAGGTG ACCACAGCCG CTCTGCCCGT TTCCTCGGGG CCAATGGGGA GGAGGTATCC
    TTCAACCAGA TGGCAGCCGC CACCATCACA GTCCCCTACG ATGGCTGCAG GCTCCGGAAG
    GGACAGTCGA AGACCCTCTT TGAGTTCAGC TCCTCCCGAG TGGGATTTCT GCCCCTGTTG
    GACGTGGCAG CTGCTGACTT TGGCCAGACG AACCAGAAGT TTGGGTGGGT TCGCAGTGAA
    GGGACTGATG TCAGACTACA GAGGGAAGTG GGACTTCCCT GGACTGAGCT GCGTACTCTT
    TCTGCTGCCT CAGCCCAGCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805115.1
    CDS153..5396
    Translation

    Target ORF information:

    RefSeq Version XM_010806813.2
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type V alpha 3 (COL5A3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806813.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCGGGAC GCAGGCCGTA GATCCTGTGG ATGTGCTGAA GGCACTGGGC
    ATGCGGGGGG GCCAGGCTGG GATCCCTGAG GGACCCGGCC TCTGCCCCCA GAGGGTCCCG
    GAGGGCGACC GAGCATTCCG GGTGGGCAAG ACTGGCACAC TTGGTGTCCC CACGCAGGAG
    CTCTTCCAAG ATGGGCACTT TCCTGAGAAC TTCTCCATCC TGATCACCCT GCGGGGCCAG
    CCGGCCAACC GTTCTGTCCT TCTGTCCATC TATGATGAGG GCGGCGTCCG GCAGCTCGGC
    TTGGCCTTGG GGCCAGCTCT GGACCTCCTA GGTGACTCCT TCAGCCCCCT CCCCCAGCAG
    GTCAACCTCA CGGATGGCAG GTGGCACCGT GTGGCAGTCA GTGTGGACGG TGGGATGGTG
    ACCCTGGTGG CTGATTGTGA ACCTCAGCTC CCCATGTTGG CTCAGGGACC TCGATTCATC
    AGTACAGCGG GACTCACTGT GCTGGGGACC CAGGACCTTG GCGAGGAGAC TTTTGAGGGA
    GATGTTCAGG AGCTGGTGAT AAGCCCAGAT CCCCAGGCTG CCTTCCAGGC ATGTGAGCGG
    TACCTTCCTG GCTGTGATGA CCTGGATCCA ACAGTCACCA CGGTCCCTCA GGGGGAGCCA
    GAAACCCCTC CCCCTAGGCG GAAGGGGAAA GGAAAAGGGA AGAAAAAAGG GCGAGGTCGT
    AAAGGGAAGG GCAGGAAGAA GAAGAACAAG GAAACTGTGG CTGTGAGTCC TCCTCCTGGC
    TCCCTGGACA ACCAGACCTC CACTGACATC CCCAAGACAG AGACACCAGA ACCAACTCTG
    TCTCCGACAC CCACGCCTTT GGTCATCACC ACAACTGTGA CCTTTGGTAG CAATGTCACC
    ATCCTAGAGG AGGGCCTGGA CCCTGACAAT GGAACTGAAC TGGAGACCCT GGAGACTGAG
    TTAGACAGAG AAGATGAAGA AGAAGGTGAT CCCACCATGG GCCCCCACTT CCGGGCAGCA
    GAACAGTCAT GGAATACTGA GTTCCAGATC TTTCCTGGTG CTGGGGAGAA AGGAGAAAAA
    GGAGAACCAG CTGTGGTCGA ACAGGGACAG CAGTTTGAAG GACCCCCAGG AGCCCCGGGA
    CCCCAAGGCC CTGCTGGCCT CCCAGGCATC CCTGGCATGG ATGGGATCCG GGGTCTCCCA
    GGCACTGTGA TCATGATGCC GTTCCAGTTT GCAAGAGGCT CTCACAAAGG ACCCCCAGTA
    TCCTTCCAGC AGGCCCAGGC TCAGGCAGTC CTGCAACAAG CTCAGCTGTC TATGAAAGGC
    CCTCCTGGCC CGGTGGGGCT CACCGGACGC CCAGGTCCCG TGGGTCTTCC TGGGTATCCA
    GGTCTGAAAG GAGAGATGGG AGAAATGGGG CCGCAGGGTC CCCGAGGGCT TCAGGGACCT
    CATGGGCCCC CTGGCCGAGA GGGAAAGATG GGCCGCGCTG GAGCAGATGG GGCTCGGGGC
    CTCCCCGGCG CTACGGGACC TAAGGGTGAT CGGGGCTTTG ATGGCCTGCC AGGGCTGCCT
    GGTGAGAAGG GCCAAAGGGG TGACTTTGGT TATGTGGGGA AACCTGGTCC CCCAGGAGAG
    GATGGACGGA AGGGAGCAGA GGGACCTCCG GGGCCCACTG GCCAGGCAGG GGAGCCGGGC
    CCCCGAGGAC TGATTGGCCC TAGAGGCTCC CCCGGACCCC TGGGACGCCC GGGTGTGATT
    GGAAGTGATG GTGCTCCAGG TGCCAAAGGA AATGTGGGTC CTCCAGGAGA ACCAGGCCCC
    CCAGGACAGC AGGGAAATCC TGGGTCCCAG GGAATTCCCG GCCCCCAGGG ACCCATCGGC
    ACTCCTGGGG AGAAGGGTCC TCCTGGAAAC CCAGGAATTC CAGGCCTCCC AGGATCTGAA
    GGCCCTCCGG GTCACCCAGG TCATGAGGGC CCAACAGGAG AGAAAGGGGC CCAGGGTCCA
    GCAGGGTCAG CAGGCCCTCC GGGCTATCCA GGACCTCGGG GTGTGAAGGG TACCTCTGGC
    AACCGGGGCC TCCAGGGGGA GAAAGGAGAG AAGGGAGAGG ATGGTTTCCC AGGCTTCAAG
    GGTGATGCGG GGCTTAAAGG AGATCGGGGG CAACCCGGAC CTCCAGGTCC TCGGGGAGAG
    GATGGTCCGG AGGGGCTAAA GGGGCAGGAG GGGCTGCTTG GCGAGGAGGG TCCCCCAGGC
    TCAGCTGGGG AGAAGGGCAA GCTTGGCGTG CCGGGTCTCC CAGGTTATCC AGGACGTCCA
    GGCCCTAAGG GATCTATTGG CTTTCCGGGA CCTCTGGGAC CATTAGGAGA AAAAGGGAAG
    CGGGGGAAGG CTGGGCAGCC AGGCCTGGAA GGAGAGCGGG GACCACCAGG TGCCCGTGGA
    GAGAGAGGGC AACCAGGTGC CACAGGGCAG CCGGGCCCCA AGGGTGATGT GGGCCAGGAC
    GGAGCCCCTG GGTTCCCTGG AGAAAAGGGC CTCCCAGGTC TGCAAGGTCC TCCTGGGTTC
    TCTGGACCAA AGGGTCCCCC TGGTCCCCAG GGAAAAGATG GGGTACCTGG ACACCCTGGA
    CAGAGAGGAG AATTGGGTTT CCAAGGTCAG ACAGGCCCAC CTGGACCAGC TGGTGTCGTG
    GGTCCTCAGG GAAAGACAGG AGAAGCAGGG CCTCTGGGTG AGAGGGGGCC CCCAGGGCCC
    CCTGGACCTC CTGGTGAACA AGGTCTTCCG GGCCTGGAAG GCAGAGAGGG GGCCAAGGGG
    GATGTGGGAC CACTGGGACC CCTTGGGAAG GAAGGGCCAC CTGGACCCAG AGGTTTCCCT
    GGTCCTCATG GAGCCCCCGG GGACCCAGGA CCGACTGGTT TAAAGGGTGA CAAGGGCCCC
    CCAGGTCCTG TTGGTGCCAA CGGATCCCCT GGGGAGCGTG GTCCTGTAGG CCCAGCAGGG
    AGCATTGGGC TTCCTGGTCA AAGCGGAGGC CAAGGCCCCG TGGGCCCCAC AGGCGAGAAG
    GGTTCCCCGG GTGAACGTGG CGCCCCTGGC CCAACTGGTA AAGATGGGAT CCCCGGGCCC
    CTGGGGCTTC CTGGGCCCCC TGGAGCTGCG GGGCCTTCTG GCGAGGAAGG GGACAAGGGA
    GAAGTGGGTG CCCCCGGTCA CAAAGGCAGC AAAGGTGACA AGGGAGATGC GGGCCCGCCT
    GGACCAACAG GGATACGGGG TCTGGTGGGA CACCCCGGCT CCCCGGGAGC AGACGGGGCT
    CAGGGACGCC GGGGACCCCC AGGCCTCTTT GGGCAGAAAG GAGATGATGG AGTGAGAGGC
    TTCCCTGGCA TGATTGGTCC TCCTGGCCTA CAGGGAATGC CAGGCCCTGC TGGAGAAAAG
    GGAGAAGTTG GAGATGTAGG GTCCATGGGT CCCCACGGAG CTCCAGGCCC TCGGGGCCCC
    CAGGGCCCCA GCGGATCAGA GGGTCCTCCA GGGTTGCCTG GGGGAGTTGG TCAGCCTGGC
    GCTGTGGGGG AGAAGGGTGA GCCAGGAGAG CCTGGAGACC CAGGACCCCC CGGGACCCCA
    GGCACCCCGG GCCCCAGGGG AGAAGTTGGT GAAAAGGGGG ATACAGGCCC ATCTGGGGCT
    GCTGGACCCC CAGGCAAGAA AGGACCCCCT GGAGAGGATG GAGCAAAGGG GAATGTGGGC
    CCCACAGGCC TCCCAGGAGA CCTAGGCCCC CCCGGAGACC CTGGAGTTTC GGGTCAAGAT
    GGCCCCCCAG GGGAGAAGGG AAACCCTGGA GATGTGGGGG GACCGGGTCC ACCTGGCGCT
    TCTGGGGAGC CTGGCGCCCC TGGGCCTCCT GGTAAAAGGG GTCCTTCTGG TCGCATGGGT
    CAAGAAGGTA GAGAAGGAGA GAAAGGTGCC AAGGGGGAAC CAGGTCCTGA TGGACCCCCA
    GGGAGGACAG GCCCAGTAGG GGCTCGAGGG CCCCCTGGAC GTGTTGGACC TGAGGGTCTT
    CGTGGGATCC CTGGACCTGT GGGCGAACCA GGCCTCCTGG GACCTCCTGG ACTGTTAGGC
    CCTCCTGGTC CCCTGGGGCC CTCTGGCCTA CCAGGGCTGA AGGGAGATGC TGGCCCCAAG
    GGAGAAAAGG GCCACATTGG ATTAATTGGT CTTGTCGGCC CCCCTGGGGA GGTTGGTGAG
    AAAGGGGATC AGGGGTTGCC AGGAGTGCAA GGCTCCCCTG GCCTCCAGGG AGAACCGGGT
    TCTCCTGGCC CCATTGGCTC TCTGGGCCAC CCTGGGCCCC AAGGCGTGGT GGGTCCTCCA
    GGACAGAAAG GCTCAAAGGG GTCCCCGGGA TCCCAAGGTC CTCGTGGAGA CGCTGGCCCC
    CCAGGTCCTC CTGGTCCCCC GGGTCCCTCT GCGGAGCTGC ACGGGCTGCG CCGGCGCCGA
    CGTTCCCTGC TAGGCTTGGG AACCCCGGAG GGCGGCCTGG AGGAGGTGCT GGCCTCGATG
    GCGTCGCTGA GCTTCGAGTT AGAGCAGCTG CGGCGCCCTC CCGGCACGGC TGAGCGCCCG
    GGCCTCGTGT GCAAAGAGTT GCACCGCAGC CATCCGCATC TGCCAGATGG GGAATACTGG
    ATTGATCCCA ATCAAGGCTG CGCGCGGGAT GCCCTCAGGG TTTTCTGTAA CTTCACGGCA
    GGAGGAGAAA CCTGCCTCTA CCCAGACAAG AAGTTTGAGA CCGTGAAACT GGCCTCTTGG
    TCCAAAGAAA AGCCAGGTGG CTGGTATAGC ACATTCCGTC GAGGGAAAAA GTTCTCCTAC
    GTGGATGCCG ATGGGGCCGC AGTGAGCGTG GTACAGCTGA CCTTCCTGAA GTTGCTGAGT
    GCCACGGCCC GCCAGAGCTT CACATTCACC TGCCAGAACT CGGCCGCCTG GCTGGACGAA
    GCTGCAGGTG ACCACAGCCG CTCTGCCCGT TTCCTCGGGG CCAATGGGGA GGAGGTATCC
    TTCAACCAGA TGGCAGCCGC CACCATCACA GTCCCCTACG ATGGCTGCAG GCTCCGGAAG
    GGACAGTCGA AGACCCTCTT TGAGTTCAGC TCCTCCCGAG TGGGATTTCT GCCCCTGTTG
    GACGTGGCAG CTGCTGACTT TGGCCAGACG AACCAGAAGT TTGGGTGGGT TCGCAGTGAA
    GGGACTGATG TCAGACTACA GAGGGAAGTG GGACTTCCCT GGACTGAGCT GCGTACTCTT
    TCTGCTGCCT CAGCCCAGCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46996
    Clone ID Related Accession (Same CDS sequence) XM_010806815.2 , XM_010806815.3
    Accession Version XM_010806815.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-3(V) chain isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806815.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGACCC TGGTGGCTGA TTGTGAACCT CAGCTCCCCA TGTTGGCTCA GGGACCTCGA 
    TTCATCAGTA CAGCGGGACT CACTGTGCTG GGGACCCAGG ACCTTGGCGA GGAGACTTTT
    GAGGGAGATG TTCAGGAGCT GGTGATAAGC CCAGATCCCC AGGCTGCCTT CCAGGCATGT
    GAGCGGTACC TTCCTGGCTG TGATGACCTG GATCCAACAG TCACCACGGT CCCTCAGGGG
    GAGCCAGAAA CCCCTCCCCC TAGGCGGAAG GGGAAAGGAA AAGGGAAGAA AAAAGGGCGA
    GGTCGTAAAG GGAAGGGCAG GAAGAAGAAG AACAAGGAAA CTGTGGCTGT GAGTCCTCCT
    CCTGGCTCCC TGGACAACCA GACCTCCACT GACATCCCCA AGACAGAGAC ACCAGAACCA
    ACTCTGTCTC CGACACCCAC GCCTTTGGTC ATCACCACAA CTGTGACCTT TGGTAGCAAT
    GTCACCATCC TAGAGGAGGG CCTGGACCCT GACAATGGAA CTGAACTGGA GACCCTGGAG
    ACTGAGTTAG ACAGAGAAGA TGAAGAAGAA GGTGATCCCA CCATGGGCCC CCACTTCCGG
    GCAGCAGAAC AGTCATGGAA TACTGAGTTC CAGATCTTTC CTGGTGCTGG GGAGAAAGGA
    GAAAAAGGAG AACCAGCTGT GGTCGAACAG GGACAGCAGT TTGAAGGACC CCCAGGAGCC
    CCGGGACCCC AAGGGGTGGT TGGCCCCTCA GGCCCTCCTG GCCCCCCAGG ATTCCCTGGA
    GACCGTGGTC TACCGGGCCC TGCTGGCCTC CCAGGCATCC CTGGCATGGA TGGGATCCGG
    GGTCTCCCAG GCACTGTGAT CATGATGCCG TTCCAGTTTG CAAGAGGCTC TCACAAAGGA
    CCCCCAGTAT CCTTCCAGCA GGCCCAGGCT CAGGCAGTCC TGCAACAAGC TCAGCTGTCT
    ATGAAAGGCC CTCCTGGCCC GGTGGGGCTC ACCGGACGCC CAGGTCCCGT GGGTCTTCCT
    GGGTATCCAG GTCTGAAAGG AGAGATGGGA GAAATGGGGC CGCAGGGTCC CCGAGGGCTT
    CAGGGACCTC ATGGGCCCCC TGGCCGAGAG GGAAAGATGG GCCGCGCTGG AGCAGATGGG
    GCTCGGGGCC TCCCCGGCGC TACGGGACCT AAGGGTGATC GGGGCTTTGA TGGCCTGCCA
    GGGCTGCCTG GTGAGAAGGG CCAAAGGGGT GACTTTGGTT ATGTGGGGAA ACCTGGTCCC
    CCAGGAGAGG ATGGACGGAA GGGAGCAGAG GGACCTCCGG GGCCCACTGG CCAGGCAGGG
    GAGCCGGGCC CCCGAGGACT GATTGGCCCT AGAGGCTCCC CCGGACCCCT GGGACGCCCG
    GGTGTGATTG GAAGTGATGG TGCTCCAGGT GCCAAAGGAA ATGTGGGTCC TCCAGGAGAA
    CCAGGCCCCC CAGGACAGCA GGGAAATCCT GGGTCCCAGG GAATTCCCGG CCCCCAGGGA
    CCCATCGGCA CTCCTGGGGA GAAGGGTCCT CCTGGAAACC CAGGAATTCC AGGCCTCCCA
    GGATCTGAAG GCCCTCCGGG TCACCCAGGT CATGAGGGCC CAACAGGAGA GAAAGGGGCC
    CAGGGTCCAG CAGGGTCAGC AGGCCCTCCG GGCTATCCAG GACCTCGGGG TGTGAAGGGT
    ACCTCTGGCA ACCGGGGCCT CCAGGGGGAG AAAGGAGAGA AGGGAGAGGA TGGTTTCCCA
    GGCTTCAAGG GTGATGCGGG GCTTAAAGGA GATCGGGGGC AACCCGGACC TCCAGGTCCT
    CGGGGAGAGG ATGGTCCGGA GGGGCTAAAG GGGCAGGAGG GGCTGCTTGG CGAGGAGGGT
    CCCCCAGGCT CAGCTGGGGA GAAGGGCAAG CTTGGCGTGC CGGGTCTCCC AGGTTATCCA
    GGACGTCCAG GCCCTAAGGG ATCTATTGGC TTTCCGGGAC CTCTGGGACC ATTAGGAGAA
    AAAGGGAAGC GGGGGAAGGC TGGGCAGCCA GGCCTGGAAG GAGAGCGGGG ACCACCAGGT
    GCCCGTGGAG AGAGAGGGCA ACCAGGTGCC ACAGGGCAGC CGGGCCCCAA GGGTGATGTG
    GGCCAGGACG GAGCCCCTGG GTTCCCTGGA GAAAAGGGCC TCCCAGGTCT GCAAGGTCCT
    CCTGGGTTCT CTGGACCAAA GGGTCCCCCT GGTCCCCAGG GAAAAGATGG GGTACCTGGA
    CACCCTGGAC AGAGAGGAGA ATTGGGTTTC CAAGGTCAGA CAGGCCCACC TGGACCAGCT
    GGTGTCGTGG GTCCTCAGGG AAAGACAGGA GAAGCAGGGC CTCTGGGTGA GAGGGGGCCC
    CCAGGGCCCC CTGGACCTCC TGGTGAACAA GGTCTTCCGG GCCTGGAAGG CAGAGAGGGG
    GCCAAGGGGG ATGTGGGACC ACTGGGACCC CTTGGGAAGG AAGGGCCACC TGGACCCAGA
    GGTTTCCCTG GTCCTCATGG AGCCCCCGGG GACCCAGGAC CGACTGGTTT AAAGGGTGAC
    AAGGGCCCCC CAGGTCCTGT TGGTGCCAAC GGATCCCCTG GGGAGCGTGG TCCTGTAGGC
    CCAGCAGGGA GCATTGGGCT TCCTGGTCAA AGCGGAGGCC AAGGCCCCGT GGGCCCCACA
    GGCGAGAAGG GTTCCCCGGG TGAACGTGGC GCCCCTGGCC CAACTGGTAA AGATGGGATC
    CCCGGGCCCC TGGGGCTTCC TGGGCCCCCT GGAGCTGCGG GGCCTTCTGG CGAGGAAGGG
    GACAAGGGAG AAGTGGGTGC CCCCGGTCAC AAAGGCAGCA AAGGTGACAA GGGAGATGCG
    GGCCCGCCTG GACCAACAGG GATACGGGGT CTGGTGGGAC ACCCCGGCTC CCCGGGAGCA
    GACGGGGCTC AGGGACGCCG GGGACCCCCA GGCCTCTTTG GGCAGAAAGG AGATGATGGA
    GTGAGAGGCT TCCCTGGCAT GATTGGTCCT CCTGGCCTAC AGGGAATGCC AGGCCCTGCT
    GGAGAAAAGG GAGAAGTTGG AGATGTAGGG TCCATGGGTC CCCACGGAGC TCCAGGCCCT
    CGGGGCCCCC AGGGCCCCAG CGGATCAGAG GGTCCTCCAG GGTTGCCTGG GGGAGTTGGT
    CAGCCTGGCG CTGTGGGGGA GAAGGGTGAG CCAGGAGAGC CTGGAGACCC AGGACCCCCC
    GGGACCCCAG GCACCCCGGG CCCCAGGGGA GAAGTTGGTG AAAAGGGGGA TACAGGCCCA
    TCTGGGGCTG CTGGACCCCC AGGCAAGAAA GGACCCCCTG GAGAGGATGG AGCAAAGGGG
    AATGTGGGCC CCACAGGCCT CCCAGGAGAC CTAGGCCCCC CCGGAGACCC TGGAGTTTCG
    GGTCAAGATG GCCCCCCAGG GGAGAAGGGA AACCCTGGAG ATGTGGGGGG ACCGGGTCCA
    CCTGGCGCTT CTGGGGAGCC TGGCGCCCCT GGGCCTCCTG GTAAAAGGGG TCCTTCTGGT
    CGCATGGGTC AAGAAGGTAG AGAAGGAGAG AAAGGTGCCA AGGGGGAACC AGGTCCTGAT
    GGACCCCCAG GGAGGACAGG CCCAGTAGGG GCTCGAGGGC CCCCTGGACG TGTTGGACCT
    GAGGGTCTTC GTGGGATCCC TGGACCTGTG GGCGAACCAG GCCTCCTGGG ACCTCCTGGA
    CTGTTAGGCC CTCCTGGTCC CCTGGGGCCC TCTGGCCTAC CAGGGCTGAA GGGAGATGCT
    GGCCCCAAGG GAGAAAAGGG CCACATTGGA TTAATTGGTC TTGTCGGCCC CCCTGGGGAG
    GTTGGTGAGA AAGGGGATCA GGGGTTGCCA GGAGTGCAAG GCTCCCCTGG CCTCCAGGGA
    GAACCGGGTT CTCCTGGCCC CATTGGCTCT CTGGGCCACC CTGGGCCCCA AGGCGTGGTG
    GGTCCTCCAG GACAGAAAGG CTCAAAGGGG TCCCCGGGAT CCCAAGGTCC TCGTGGAGAC
    GCTGGCCCCC CAGGTCCTCC TGGTCCCCCG GGTCCCTCTG CGGAGCTGCA CGGGCTGCGC
    CGGCGCCGAC GTTCCCTGCT AGGCTTGGGA ACCCCGGAGG GCGGCCTGGA GGAGGTGCTG
    GCCTCGATGG CGTCGCTGAG CTTCGAGTTA GAGCAGCTGC GGCGCCCTCC CGGCACGGCT
    GAGCGCCCGG GCCTCGTGTG CAAAGAGTTG CACCGCAGCC ATCCGCATCT GCCAGATGGG
    GAATACTGGA TTGATCCCAA TCAAGGCTGC GCGCGGGATG CCCTCAGGGT TTTCTGTAAC
    TTCACGGCAG GAGGAGAAAC CTGCCTCTAC CCAGACAAGA AGTTTGAGAC CGTGAAACTG
    GCCTCTTGGT CCAAAGAAAA GCCAGGTGGC TGGTATAGCA CATTCCGTCG AGGGAAAAAG
    TTCTCCTACG TGGATGCCGA TGGGGCCGCA GTGAGCGTGG TACAGCTGAC CTTCCTGAAG
    TTGCTGAGTG CCACGGCCCG CCAGAGCTTC ACATTCACCT GCCAGAACTC GGCCGCCTGG
    CTGGACGAAG CTGCAGGTGA CCACAGCCGC TCTGCCCGTT TCCTCGGGGC CAATGGGGAG
    GAGGTATCCT TCAACCAGAT GGCAGCCGCC ACCATCACAG TCCCCTACGA TGGCTGCAGG
    CTCCGGAAGG GACAGTCGAA GACCCTCTTT GAGTTCAGCT CCTCCCGAGT GGGATTTCTG
    CCCCTGTTGG ACGTGGCAGC TGCTGACTTT GGCCAGACGA ACCAGAAGTT TGGGTGGGTT
    CGCAGTGAAG GGACTGATGT CAGACTACAG AGGGAAGTGG GACTTCCCTG GACTGAGCTG
    CGTACTCTTT CTGCTGCCTC AGCCCAGCTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805117.1
    CDS124..4956
    Translation

    Target ORF information:

    RefSeq Version XM_010806815.2
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type V alpha 3 (COL5A3), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806815.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGACCC TGGTGGCTGA TTGTGAACCT CAGCTCCCCA TGTTGGCTCA GGGACCTCGA 
    TTCATCAGTA CAGCGGGACT CACTGTGCTG GGGACCCAGG ACCTTGGCGA GGAGACTTTT
    GAGGGAGATG TTCAGGAGCT GGTGATAAGC CCAGATCCCC AGGCTGCCTT CCAGGCATGT
    GAGCGGTACC TTCCTGGCTG TGATGACCTG GATCCAACAG TCACCACGGT CCCTCAGGGG
    GAGCCAGAAA CCCCTCCCCC TAGGCGGAAG GGGAAAGGAA AAGGGAAGAA AAAAGGGCGA
    GGTCGTAAAG GGAAGGGCAG GAAGAAGAAG AACAAGGAAA CTGTGGCTGT GAGTCCTCCT
    CCTGGCTCCC TGGACAACCA GACCTCCACT GACATCCCCA AGACAGAGAC ACCAGAACCA
    ACTCTGTCTC CGACACCCAC GCCTTTGGTC ATCACCACAA CTGTGACCTT TGGTAGCAAT
    GTCACCATCC TAGAGGAGGG CCTGGACCCT GACAATGGAA CTGAACTGGA GACCCTGGAG
    ACTGAGTTAG ACAGAGAAGA TGAAGAAGAA GGTGATCCCA CCATGGGCCC CCACTTCCGG
    GCAGCAGAAC AGTCATGGAA TACTGAGTTC CAGATCTTTC CTGGTGCTGG GGAGAAAGGA
    GAAAAAGGAG AACCAGCTGT GGTCGAACAG GGACAGCAGT TTGAAGGACC CCCAGGAGCC
    CCGGGACCCC AAGGGGTGGT TGGCCCCTCA GGCCCTCCTG GCCCCCCAGG ATTCCCTGGA
    GACCGTGGTC TACCGGGCCC TGCTGGCCTC CCAGGCATCC CTGGCATGGA TGGGATCCGG
    GGTCTCCCAG GCACTGTGAT CATGATGCCG TTCCAGTTTG CAAGAGGCTC TCACAAAGGA
    CCCCCAGTAT CCTTCCAGCA GGCCCAGGCT CAGGCAGTCC TGCAACAAGC TCAGCTGTCT
    ATGAAAGGCC CTCCTGGCCC GGTGGGGCTC ACCGGACGCC CAGGTCCCGT GGGTCTTCCT
    GGGTATCCAG GTCTGAAAGG AGAGATGGGA GAAATGGGGC CGCAGGGTCC CCGAGGGCTT
    CAGGGACCTC ATGGGCCCCC TGGCCGAGAG GGAAAGATGG GCCGCGCTGG AGCAGATGGG
    GCTCGGGGCC TCCCCGGCGC TACGGGACCT AAGGGTGATC GGGGCTTTGA TGGCCTGCCA
    GGGCTGCCTG GTGAGAAGGG CCAAAGGGGT GACTTTGGTT ATGTGGGGAA ACCTGGTCCC
    CCAGGAGAGG ATGGACGGAA GGGAGCAGAG GGACCTCCGG GGCCCACTGG CCAGGCAGGG
    GAGCCGGGCC CCCGAGGACT GATTGGCCCT AGAGGCTCCC CCGGACCCCT GGGACGCCCG
    GGTGTGATTG GAAGTGATGG TGCTCCAGGT GCCAAAGGAA ATGTGGGTCC TCCAGGAGAA
    CCAGGCCCCC CAGGACAGCA GGGAAATCCT GGGTCCCAGG GAATTCCCGG CCCCCAGGGA
    CCCATCGGCA CTCCTGGGGA GAAGGGTCCT CCTGGAAACC CAGGAATTCC AGGCCTCCCA
    GGATCTGAAG GCCCTCCGGG TCACCCAGGT CATGAGGGCC CAACAGGAGA GAAAGGGGCC
    CAGGGTCCAG CAGGGTCAGC AGGCCCTCCG GGCTATCCAG GACCTCGGGG TGTGAAGGGT
    ACCTCTGGCA ACCGGGGCCT CCAGGGGGAG AAAGGAGAGA AGGGAGAGGA TGGTTTCCCA
    GGCTTCAAGG GTGATGCGGG GCTTAAAGGA GATCGGGGGC AACCCGGACC TCCAGGTCCT
    CGGGGAGAGG ATGGTCCGGA GGGGCTAAAG GGGCAGGAGG GGCTGCTTGG CGAGGAGGGT
    CCCCCAGGCT CAGCTGGGGA GAAGGGCAAG CTTGGCGTGC CGGGTCTCCC AGGTTATCCA
    GGACGTCCAG GCCCTAAGGG ATCTATTGGC TTTCCGGGAC CTCTGGGACC ATTAGGAGAA
    AAAGGGAAGC GGGGGAAGGC TGGGCAGCCA GGCCTGGAAG GAGAGCGGGG ACCACCAGGT
    GCCCGTGGAG AGAGAGGGCA ACCAGGTGCC ACAGGGCAGC CGGGCCCCAA GGGTGATGTG
    GGCCAGGACG GAGCCCCTGG GTTCCCTGGA GAAAAGGGCC TCCCAGGTCT GCAAGGTCCT
    CCTGGGTTCT CTGGACCAAA GGGTCCCCCT GGTCCCCAGG GAAAAGATGG GGTACCTGGA
    CACCCTGGAC AGAGAGGAGA ATTGGGTTTC CAAGGTCAGA CAGGCCCACC TGGACCAGCT
    GGTGTCGTGG GTCCTCAGGG AAAGACAGGA GAAGCAGGGC CTCTGGGTGA GAGGGGGCCC
    CCAGGGCCCC CTGGACCTCC TGGTGAACAA GGTCTTCCGG GCCTGGAAGG CAGAGAGGGG
    GCCAAGGGGG ATGTGGGACC ACTGGGACCC CTTGGGAAGG AAGGGCCACC TGGACCCAGA
    GGTTTCCCTG GTCCTCATGG AGCCCCCGGG GACCCAGGAC CGACTGGTTT AAAGGGTGAC
    AAGGGCCCCC CAGGTCCTGT TGGTGCCAAC GGATCCCCTG GGGAGCGTGG TCCTGTAGGC
    CCAGCAGGGA GCATTGGGCT TCCTGGTCAA AGCGGAGGCC AAGGCCCCGT GGGCCCCACA
    GGCGAGAAGG GTTCCCCGGG TGAACGTGGC GCCCCTGGCC CAACTGGTAA AGATGGGATC
    CCCGGGCCCC TGGGGCTTCC TGGGCCCCCT GGAGCTGCGG GGCCTTCTGG CGAGGAAGGG
    GACAAGGGAG AAGTGGGTGC CCCCGGTCAC AAAGGCAGCA AAGGTGACAA GGGAGATGCG
    GGCCCGCCTG GACCAACAGG GATACGGGGT CTGGTGGGAC ACCCCGGCTC CCCGGGAGCA
    GACGGGGCTC AGGGACGCCG GGGACCCCCA GGCCTCTTTG GGCAGAAAGG AGATGATGGA
    GTGAGAGGCT TCCCTGGCAT GATTGGTCCT CCTGGCCTAC AGGGAATGCC AGGCCCTGCT
    GGAGAAAAGG GAGAAGTTGG AGATGTAGGG TCCATGGGTC CCCACGGAGC TCCAGGCCCT
    CGGGGCCCCC AGGGCCCCAG CGGATCAGAG GGTCCTCCAG GGTTGCCTGG GGGAGTTGGT
    CAGCCTGGCG CTGTGGGGGA GAAGGGTGAG CCAGGAGAGC CTGGAGACCC AGGACCCCCC
    GGGACCCCAG GCACCCCGGG CCCCAGGGGA GAAGTTGGTG AAAAGGGGGA TACAGGCCCA
    TCTGGGGCTG CTGGACCCCC AGGCAAGAAA GGACCCCCTG GAGAGGATGG AGCAAAGGGG
    AATGTGGGCC CCACAGGCCT CCCAGGAGAC CTAGGCCCCC CCGGAGACCC TGGAGTTTCG
    GGTCAAGATG GCCCCCCAGG GGAGAAGGGA AACCCTGGAG ATGTGGGGGG ACCGGGTCCA
    CCTGGCGCTT CTGGGGAGCC TGGCGCCCCT GGGCCTCCTG GTAAAAGGGG TCCTTCTGGT
    CGCATGGGTC AAGAAGGTAG AGAAGGAGAG AAAGGTGCCA AGGGGGAACC AGGTCCTGAT
    GGACCCCCAG GGAGGACAGG CCCAGTAGGG GCTCGAGGGC CCCCTGGACG TGTTGGACCT
    GAGGGTCTTC GTGGGATCCC TGGACCTGTG GGCGAACCAG GCCTCCTGGG ACCTCCTGGA
    CTGTTAGGCC CTCCTGGTCC CCTGGGGCCC TCTGGCCTAC CAGGGCTGAA GGGAGATGCT
    GGCCCCAAGG GAGAAAAGGG CCACATTGGA TTAATTGGTC TTGTCGGCCC CCCTGGGGAG
    GTTGGTGAGA AAGGGGATCA GGGGTTGCCA GGAGTGCAAG GCTCCCCTGG CCTCCAGGGA
    GAACCGGGTT CTCCTGGCCC CATTGGCTCT CTGGGCCACC CTGGGCCCCA AGGCGTGGTG
    GGTCCTCCAG GACAGAAAGG CTCAAAGGGG TCCCCGGGAT CCCAAGGTCC TCGTGGAGAC
    GCTGGCCCCC CAGGTCCTCC TGGTCCCCCG GGTCCCTCTG CGGAGCTGCA CGGGCTGCGC
    CGGCGCCGAC GTTCCCTGCT AGGCTTGGGA ACCCCGGAGG GCGGCCTGGA GGAGGTGCTG
    GCCTCGATGG CGTCGCTGAG CTTCGAGTTA GAGCAGCTGC GGCGCCCTCC CGGCACGGCT
    GAGCGCCCGG GCCTCGTGTG CAAAGAGTTG CACCGCAGCC ATCCGCATCT GCCAGATGGG
    GAATACTGGA TTGATCCCAA TCAAGGCTGC GCGCGGGATG CCCTCAGGGT TTTCTGTAAC
    TTCACGGCAG GAGGAGAAAC CTGCCTCTAC CCAGACAAGA AGTTTGAGAC CGTGAAACTG
    GCCTCTTGGT CCAAAGAAAA GCCAGGTGGC TGGTATAGCA CATTCCGTCG AGGGAAAAAG
    TTCTCCTACG TGGATGCCGA TGGGGCCGCA GTGAGCGTGG TACAGCTGAC CTTCCTGAAG
    TTGCTGAGTG CCACGGCCCG CCAGAGCTTC ACATTCACCT GCCAGAACTC GGCCGCCTGG
    CTGGACGAAG CTGCAGGTGA CCACAGCCGC TCTGCCCGTT TCCTCGGGGC CAATGGGGAG
    GAGGTATCCT TCAACCAGAT GGCAGCCGCC ACCATCACAG TCCCCTACGA TGGCTGCAGG
    CTCCGGAAGG GACAGTCGAA GACCCTCTTT GAGTTCAGCT CCTCCCGAGT GGGATTTCTG
    CCCCTGTTGG ACGTGGCAGC TGCTGACTTT GGCCAGACGA ACCAGAAGTT TGGGTGGGTT
    CGCAGTGAAG GGACTGATGT CAGACTACAG AGGGAAGTGG GACTTCCCTG GACTGAGCTG
    CGTACTCTTT CTGCTGCCTC AGCCCAGCTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46996
    Clone ID Related Accession (Same CDS sequence) XM_010806815.2 , XM_010806815.3
    Accession Version XM_010806815.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-3(V) chain isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806815.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGACCC TGGTGGCTGA TTGTGAACCT CAGCTCCCCA TGTTGGCTCA GGGACCTCGA 
    TTCATCAGTA CAGCGGGACT CACTGTGCTG GGGACCCAGG ACCTTGGCGA GGAGACTTTT
    GAGGGAGATG TTCAGGAGCT GGTGATAAGC CCAGATCCCC AGGCTGCCTT CCAGGCATGT
    GAGCGGTACC TTCCTGGCTG TGATGACCTG GATCCAACAG TCACCACGGT CCCTCAGGGG
    GAGCCAGAAA CCCCTCCCCC TAGGCGGAAG GGGAAAGGAA AAGGGAAGAA AAAAGGGCGA
    GGTCGTAAAG GGAAGGGCAG GAAGAAGAAG AACAAGGAAA CTGTGGCTGT GAGTCCTCCT
    CCTGGCTCCC TGGACAACCA GACCTCCACT GACATCCCCA AGACAGAGAC ACCAGAACCA
    ACTCTGTCTC CGACACCCAC GCCTTTGGTC ATCACCACAA CTGTGACCTT TGGTAGCAAT
    GTCACCATCC TAGAGGAGGG CCTGGACCCT GACAATGGAA CTGAACTGGA GACCCTGGAG
    ACTGAGTTAG ACAGAGAAGA TGAAGAAGAA GGTGATCCCA CCATGGGCCC CCACTTCCGG
    GCAGCAGAAC AGTCATGGAA TACTGAGTTC CAGATCTTTC CTGGTGCTGG GGAGAAAGGA
    GAAAAAGGAG AACCAGCTGT GGTCGAACAG GGACAGCAGT TTGAAGGACC CCCAGGAGCC
    CCGGGACCCC AAGGGGTGGT TGGCCCCTCA GGCCCTCCTG GCCCCCCAGG ATTCCCTGGA
    GACCGTGGTC TACCGGGCCC TGCTGGCCTC CCAGGCATCC CTGGCATGGA TGGGATCCGG
    GGTCTCCCAG GCACTGTGAT CATGATGCCG TTCCAGTTTG CAAGAGGCTC TCACAAAGGA
    CCCCCAGTAT CCTTCCAGCA GGCCCAGGCT CAGGCAGTCC TGCAACAAGC TCAGCTGTCT
    ATGAAAGGCC CTCCTGGCCC GGTGGGGCTC ACCGGACGCC CAGGTCCCGT GGGTCTTCCT
    GGGTATCCAG GTCTGAAAGG AGAGATGGGA GAAATGGGGC CGCAGGGTCC CCGAGGGCTT
    CAGGGACCTC ATGGGCCCCC TGGCCGAGAG GGAAAGATGG GCCGCGCTGG AGCAGATGGG
    GCTCGGGGCC TCCCCGGCGC TACGGGACCT AAGGGTGATC GGGGCTTTGA TGGCCTGCCA
    GGGCTGCCTG GTGAGAAGGG CCAAAGGGGT GACTTTGGTT ATGTGGGGAA ACCTGGTCCC
    CCAGGAGAGG ATGGACGGAA GGGAGCAGAG GGACCTCCGG GGCCCACTGG CCAGGCAGGG
    GAGCCGGGCC CCCGAGGACT GATTGGCCCT AGAGGCTCCC CCGGACCCCT GGGACGCCCG
    GGTGTGATTG GAAGTGATGG TGCTCCAGGT GCCAAAGGAA ATGTGGGTCC TCCAGGAGAA
    CCAGGCCCCC CAGGACAGCA GGGAAATCCT GGGTCCCAGG GAATTCCCGG CCCCCAGGGA
    CCCATCGGCA CTCCTGGGGA GAAGGGTCCT CCTGGAAACC CAGGAATTCC AGGCCTCCCA
    GGATCTGAAG GCCCTCCGGG TCACCCAGGT CATGAGGGCC CAACAGGAGA GAAAGGGGCC
    CAGGGTCCAG CAGGGTCAGC AGGCCCTCCG GGCTATCCAG GACCTCGGGG TGTGAAGGGT
    ACCTCTGGCA ACCGGGGCCT CCAGGGGGAG AAAGGAGAGA AGGGAGAGGA TGGTTTCCCA
    GGCTTCAAGG GTGATGCGGG GCTTAAAGGA GATCGGGGGC AACCCGGACC TCCAGGTCCT
    CGGGGAGAGG ATGGTCCGGA GGGGCTAAAG GGGCAGGAGG GGCTGCTTGG CGAGGAGGGT
    CCCCCAGGCT CAGCTGGGGA GAAGGGCAAG CTTGGCGTGC CGGGTCTCCC AGGTTATCCA
    GGACGTCCAG GCCCTAAGGG ATCTATTGGC TTTCCGGGAC CTCTGGGACC ATTAGGAGAA
    AAAGGGAAGC GGGGGAAGGC TGGGCAGCCA GGCCTGGAAG GAGAGCGGGG ACCACCAGGT
    GCCCGTGGAG AGAGAGGGCA ACCAGGTGCC ACAGGGCAGC CGGGCCCCAA GGGTGATGTG
    GGCCAGGACG GAGCCCCTGG GTTCCCTGGA GAAAAGGGCC TCCCAGGTCT GCAAGGTCCT
    CCTGGGTTCT CTGGACCAAA GGGTCCCCCT GGTCCCCAGG GAAAAGATGG GGTACCTGGA
    CACCCTGGAC AGAGAGGAGA ATTGGGTTTC CAAGGTCAGA CAGGCCCACC TGGACCAGCT
    GGTGTCGTGG GTCCTCAGGG AAAGACAGGA GAAGCAGGGC CTCTGGGTGA GAGGGGGCCC
    CCAGGGCCCC CTGGACCTCC TGGTGAACAA GGTCTTCCGG GCCTGGAAGG CAGAGAGGGG
    GCCAAGGGGG ATGTGGGACC ACTGGGACCC CTTGGGAAGG AAGGGCCACC TGGACCCAGA
    GGTTTCCCTG GTCCTCATGG AGCCCCCGGG GACCCAGGAC CGACTGGTTT AAAGGGTGAC
    AAGGGCCCCC CAGGTCCTGT TGGTGCCAAC GGATCCCCTG GGGAGCGTGG TCCTGTAGGC
    CCAGCAGGGA GCATTGGGCT TCCTGGTCAA AGCGGAGGCC AAGGCCCCGT GGGCCCCACA
    GGCGAGAAGG GTTCCCCGGG TGAACGTGGC GCCCCTGGCC CAACTGGTAA AGATGGGATC
    CCCGGGCCCC TGGGGCTTCC TGGGCCCCCT GGAGCTGCGG GGCCTTCTGG CGAGGAAGGG
    GACAAGGGAG AAGTGGGTGC CCCCGGTCAC AAAGGCAGCA AAGGTGACAA GGGAGATGCG
    GGCCCGCCTG GACCAACAGG GATACGGGGT CTGGTGGGAC ACCCCGGCTC CCCGGGAGCA
    GACGGGGCTC AGGGACGCCG GGGACCCCCA GGCCTCTTTG GGCAGAAAGG AGATGATGGA
    GTGAGAGGCT TCCCTGGCAT GATTGGTCCT CCTGGCCTAC AGGGAATGCC AGGCCCTGCT
    GGAGAAAAGG GAGAAGTTGG AGATGTAGGG TCCATGGGTC CCCACGGAGC TCCAGGCCCT
    CGGGGCCCCC AGGGCCCCAG CGGATCAGAG GGTCCTCCAG GGTTGCCTGG GGGAGTTGGT
    CAGCCTGGCG CTGTGGGGGA GAAGGGTGAG CCAGGAGAGC CTGGAGACCC AGGACCCCCC
    GGGACCCCAG GCACCCCGGG CCCCAGGGGA GAAGTTGGTG AAAAGGGGGA TACAGGCCCA
    TCTGGGGCTG CTGGACCCCC AGGCAAGAAA GGACCCCCTG GAGAGGATGG AGCAAAGGGG
    AATGTGGGCC CCACAGGCCT CCCAGGAGAC CTAGGCCCCC CCGGAGACCC TGGAGTTTCG
    GGTCAAGATG GCCCCCCAGG GGAGAAGGGA AACCCTGGAG ATGTGGGGGG ACCGGGTCCA
    CCTGGCGCTT CTGGGGAGCC TGGCGCCCCT GGGCCTCCTG GTAAAAGGGG TCCTTCTGGT
    CGCATGGGTC AAGAAGGTAG AGAAGGAGAG AAAGGTGCCA AGGGGGAACC AGGTCCTGAT
    GGACCCCCAG GGAGGACAGG CCCAGTAGGG GCTCGAGGGC CCCCTGGACG TGTTGGACCT
    GAGGGTCTTC GTGGGATCCC TGGACCTGTG GGCGAACCAG GCCTCCTGGG ACCTCCTGGA
    CTGTTAGGCC CTCCTGGTCC CCTGGGGCCC TCTGGCCTAC CAGGGCTGAA GGGAGATGCT
    GGCCCCAAGG GAGAAAAGGG CCACATTGGA TTAATTGGTC TTGTCGGCCC CCCTGGGGAG
    GTTGGTGAGA AAGGGGATCA GGGGTTGCCA GGAGTGCAAG GCTCCCCTGG CCTCCAGGGA
    GAACCGGGTT CTCCTGGCCC CATTGGCTCT CTGGGCCACC CTGGGCCCCA AGGCGTGGTG
    GGTCCTCCAG GACAGAAAGG CTCAAAGGGG TCCCCGGGAT CCCAAGGTCC TCGTGGAGAC
    GCTGGCCCCC CAGGTCCTCC TGGTCCCCCG GGTCCCTCTG CGGAGCTGCA CGGGCTGCGC
    CGGCGCCGAC GTTCCCTGCT AGGCTTGGGA ACCCCGGAGG GCGGCCTGGA GGAGGTGCTG
    GCCTCGATGG CGTCGCTGAG CTTCGAGTTA GAGCAGCTGC GGCGCCCTCC CGGCACGGCT
    GAGCGCCCGG GCCTCGTGTG CAAAGAGTTG CACCGCAGCC ATCCGCATCT GCCAGATGGG
    GAATACTGGA TTGATCCCAA TCAAGGCTGC GCGCGGGATG CCCTCAGGGT TTTCTGTAAC
    TTCACGGCAG GAGGAGAAAC CTGCCTCTAC CCAGACAAGA AGTTTGAGAC CGTGAAACTG
    GCCTCTTGGT CCAAAGAAAA GCCAGGTGGC TGGTATAGCA CATTCCGTCG AGGGAAAAAG
    TTCTCCTACG TGGATGCCGA TGGGGCCGCA GTGAGCGTGG TACAGCTGAC CTTCCTGAAG
    TTGCTGAGTG CCACGGCCCG CCAGAGCTTC ACATTCACCT GCCAGAACTC GGCCGCCTGG
    CTGGACGAAG CTGCAGGTGA CCACAGCCGC TCTGCCCGTT TCCTCGGGGC CAATGGGGAG
    GAGGTATCCT TCAACCAGAT GGCAGCCGCC ACCATCACAG TCCCCTACGA TGGCTGCAGG
    CTCCGGAAGG GACAGTCGAA GACCCTCTTT GAGTTCAGCT CCTCCCGAGT GGGATTTCTG
    CCCCTGTTGG ACGTGGCAGC TGCTGACTTT GGCCAGACGA ACCAGAAGTT TGGGTGGGTT
    CGCAGTGAAG GGACTGATGT CAGACTACAG AGGGAAGTGG GACTTCCCTG GACTGAGCTG
    CGTACTCTTT CTGCTGCCTC AGCCCAGCTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805117.1
    CDS153..4985
    Translation

    Target ORF information:

    RefSeq Version XM_010806815.3
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type V alpha 3 chain (COL5A3), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806815.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGACCC TGGTGGCTGA TTGTGAACCT CAGCTCCCCA TGTTGGCTCA GGGACCTCGA 
    TTCATCAGTA CAGCGGGACT CACTGTGCTG GGGACCCAGG ACCTTGGCGA GGAGACTTTT
    GAGGGAGATG TTCAGGAGCT GGTGATAAGC CCAGATCCCC AGGCTGCCTT CCAGGCATGT
    GAGCGGTACC TTCCTGGCTG TGATGACCTG GATCCAACAG TCACCACGGT CCCTCAGGGG
    GAGCCAGAAA CCCCTCCCCC TAGGCGGAAG GGGAAAGGAA AAGGGAAGAA AAAAGGGCGA
    GGTCGTAAAG GGAAGGGCAG GAAGAAGAAG AACAAGGAAA CTGTGGCTGT GAGTCCTCCT
    CCTGGCTCCC TGGACAACCA GACCTCCACT GACATCCCCA AGACAGAGAC ACCAGAACCA
    ACTCTGTCTC CGACACCCAC GCCTTTGGTC ATCACCACAA CTGTGACCTT TGGTAGCAAT
    GTCACCATCC TAGAGGAGGG CCTGGACCCT GACAATGGAA CTGAACTGGA GACCCTGGAG
    ACTGAGTTAG ACAGAGAAGA TGAAGAAGAA GGTGATCCCA CCATGGGCCC CCACTTCCGG
    GCAGCAGAAC AGTCATGGAA TACTGAGTTC CAGATCTTTC CTGGTGCTGG GGAGAAAGGA
    GAAAAAGGAG AACCAGCTGT GGTCGAACAG GGACAGCAGT TTGAAGGACC CCCAGGAGCC
    CCGGGACCCC AAGGGGTGGT TGGCCCCTCA GGCCCTCCTG GCCCCCCAGG ATTCCCTGGA
    GACCGTGGTC TACCGGGCCC TGCTGGCCTC CCAGGCATCC CTGGCATGGA TGGGATCCGG
    GGTCTCCCAG GCACTGTGAT CATGATGCCG TTCCAGTTTG CAAGAGGCTC TCACAAAGGA
    CCCCCAGTAT CCTTCCAGCA GGCCCAGGCT CAGGCAGTCC TGCAACAAGC TCAGCTGTCT
    ATGAAAGGCC CTCCTGGCCC GGTGGGGCTC ACCGGACGCC CAGGTCCCGT GGGTCTTCCT
    GGGTATCCAG GTCTGAAAGG AGAGATGGGA GAAATGGGGC CGCAGGGTCC CCGAGGGCTT
    CAGGGACCTC ATGGGCCCCC TGGCCGAGAG GGAAAGATGG GCCGCGCTGG AGCAGATGGG
    GCTCGGGGCC TCCCCGGCGC TACGGGACCT AAGGGTGATC GGGGCTTTGA TGGCCTGCCA
    GGGCTGCCTG GTGAGAAGGG CCAAAGGGGT GACTTTGGTT ATGTGGGGAA ACCTGGTCCC
    CCAGGAGAGG ATGGACGGAA GGGAGCAGAG GGACCTCCGG GGCCCACTGG CCAGGCAGGG
    GAGCCGGGCC CCCGAGGACT GATTGGCCCT AGAGGCTCCC CCGGACCCCT GGGACGCCCG
    GGTGTGATTG GAAGTGATGG TGCTCCAGGT GCCAAAGGAA ATGTGGGTCC TCCAGGAGAA
    CCAGGCCCCC CAGGACAGCA GGGAAATCCT GGGTCCCAGG GAATTCCCGG CCCCCAGGGA
    CCCATCGGCA CTCCTGGGGA GAAGGGTCCT CCTGGAAACC CAGGAATTCC AGGCCTCCCA
    GGATCTGAAG GCCCTCCGGG TCACCCAGGT CATGAGGGCC CAACAGGAGA GAAAGGGGCC
    CAGGGTCCAG CAGGGTCAGC AGGCCCTCCG GGCTATCCAG GACCTCGGGG TGTGAAGGGT
    ACCTCTGGCA ACCGGGGCCT CCAGGGGGAG AAAGGAGAGA AGGGAGAGGA TGGTTTCCCA
    GGCTTCAAGG GTGATGCGGG GCTTAAAGGA GATCGGGGGC AACCCGGACC TCCAGGTCCT
    CGGGGAGAGG ATGGTCCGGA GGGGCTAAAG GGGCAGGAGG GGCTGCTTGG CGAGGAGGGT
    CCCCCAGGCT CAGCTGGGGA GAAGGGCAAG CTTGGCGTGC CGGGTCTCCC AGGTTATCCA
    GGACGTCCAG GCCCTAAGGG ATCTATTGGC TTTCCGGGAC CTCTGGGACC ATTAGGAGAA
    AAAGGGAAGC GGGGGAAGGC TGGGCAGCCA GGCCTGGAAG GAGAGCGGGG ACCACCAGGT
    GCCCGTGGAG AGAGAGGGCA ACCAGGTGCC ACAGGGCAGC CGGGCCCCAA GGGTGATGTG
    GGCCAGGACG GAGCCCCTGG GTTCCCTGGA GAAAAGGGCC TCCCAGGTCT GCAAGGTCCT
    CCTGGGTTCT CTGGACCAAA GGGTCCCCCT GGTCCCCAGG GAAAAGATGG GGTACCTGGA
    CACCCTGGAC AGAGAGGAGA ATTGGGTTTC CAAGGTCAGA CAGGCCCACC TGGACCAGCT
    GGTGTCGTGG GTCCTCAGGG AAAGACAGGA GAAGCAGGGC CTCTGGGTGA GAGGGGGCCC
    CCAGGGCCCC CTGGACCTCC TGGTGAACAA GGTCTTCCGG GCCTGGAAGG CAGAGAGGGG
    GCCAAGGGGG ATGTGGGACC ACTGGGACCC CTTGGGAAGG AAGGGCCACC TGGACCCAGA
    GGTTTCCCTG GTCCTCATGG AGCCCCCGGG GACCCAGGAC CGACTGGTTT AAAGGGTGAC
    AAGGGCCCCC CAGGTCCTGT TGGTGCCAAC GGATCCCCTG GGGAGCGTGG TCCTGTAGGC
    CCAGCAGGGA GCATTGGGCT TCCTGGTCAA AGCGGAGGCC AAGGCCCCGT GGGCCCCACA
    GGCGAGAAGG GTTCCCCGGG TGAACGTGGC GCCCCTGGCC CAACTGGTAA AGATGGGATC
    CCCGGGCCCC TGGGGCTTCC TGGGCCCCCT GGAGCTGCGG GGCCTTCTGG CGAGGAAGGG
    GACAAGGGAG AAGTGGGTGC CCCCGGTCAC AAAGGCAGCA AAGGTGACAA GGGAGATGCG
    GGCCCGCCTG GACCAACAGG GATACGGGGT CTGGTGGGAC ACCCCGGCTC CCCGGGAGCA
    GACGGGGCTC AGGGACGCCG GGGACCCCCA GGCCTCTTTG GGCAGAAAGG AGATGATGGA
    GTGAGAGGCT TCCCTGGCAT GATTGGTCCT CCTGGCCTAC AGGGAATGCC AGGCCCTGCT
    GGAGAAAAGG GAGAAGTTGG AGATGTAGGG TCCATGGGTC CCCACGGAGC TCCAGGCCCT
    CGGGGCCCCC AGGGCCCCAG CGGATCAGAG GGTCCTCCAG GGTTGCCTGG GGGAGTTGGT
    CAGCCTGGCG CTGTGGGGGA GAAGGGTGAG CCAGGAGAGC CTGGAGACCC AGGACCCCCC
    GGGACCCCAG GCACCCCGGG CCCCAGGGGA GAAGTTGGTG AAAAGGGGGA TACAGGCCCA
    TCTGGGGCTG CTGGACCCCC AGGCAAGAAA GGACCCCCTG GAGAGGATGG AGCAAAGGGG
    AATGTGGGCC CCACAGGCCT CCCAGGAGAC CTAGGCCCCC CCGGAGACCC TGGAGTTTCG
    GGTCAAGATG GCCCCCCAGG GGAGAAGGGA AACCCTGGAG ATGTGGGGGG ACCGGGTCCA
    CCTGGCGCTT CTGGGGAGCC TGGCGCCCCT GGGCCTCCTG GTAAAAGGGG TCCTTCTGGT
    CGCATGGGTC AAGAAGGTAG AGAAGGAGAG AAAGGTGCCA AGGGGGAACC AGGTCCTGAT
    GGACCCCCAG GGAGGACAGG CCCAGTAGGG GCTCGAGGGC CCCCTGGACG TGTTGGACCT
    GAGGGTCTTC GTGGGATCCC TGGACCTGTG GGCGAACCAG GCCTCCTGGG ACCTCCTGGA
    CTGTTAGGCC CTCCTGGTCC CCTGGGGCCC TCTGGCCTAC CAGGGCTGAA GGGAGATGCT
    GGCCCCAAGG GAGAAAAGGG CCACATTGGA TTAATTGGTC TTGTCGGCCC CCCTGGGGAG
    GTTGGTGAGA AAGGGGATCA GGGGTTGCCA GGAGTGCAAG GCTCCCCTGG CCTCCAGGGA
    GAACCGGGTT CTCCTGGCCC CATTGGCTCT CTGGGCCACC CTGGGCCCCA AGGCGTGGTG
    GGTCCTCCAG GACAGAAAGG CTCAAAGGGG TCCCCGGGAT CCCAAGGTCC TCGTGGAGAC
    GCTGGCCCCC CAGGTCCTCC TGGTCCCCCG GGTCCCTCTG CGGAGCTGCA CGGGCTGCGC
    CGGCGCCGAC GTTCCCTGCT AGGCTTGGGA ACCCCGGAGG GCGGCCTGGA GGAGGTGCTG
    GCCTCGATGG CGTCGCTGAG CTTCGAGTTA GAGCAGCTGC GGCGCCCTCC CGGCACGGCT
    GAGCGCCCGG GCCTCGTGTG CAAAGAGTTG CACCGCAGCC ATCCGCATCT GCCAGATGGG
    GAATACTGGA TTGATCCCAA TCAAGGCTGC GCGCGGGATG CCCTCAGGGT TTTCTGTAAC
    TTCACGGCAG GAGGAGAAAC CTGCCTCTAC CCAGACAAGA AGTTTGAGAC CGTGAAACTG
    GCCTCTTGGT CCAAAGAAAA GCCAGGTGGC TGGTATAGCA CATTCCGTCG AGGGAAAAAG
    TTCTCCTACG TGGATGCCGA TGGGGCCGCA GTGAGCGTGG TACAGCTGAC CTTCCTGAAG
    TTGCTGAGTG CCACGGCCCG CCAGAGCTTC ACATTCACCT GCCAGAACTC GGCCGCCTGG
    CTGGACGAAG CTGCAGGTGA CCACAGCCGC TCTGCCCGTT TCCTCGGGGC CAATGGGGAG
    GAGGTATCCT TCAACCAGAT GGCAGCCGCC ACCATCACAG TCCCCTACGA TGGCTGCAGG
    CTCCGGAAGG GACAGTCGAA GACCCTCTTT GAGTTCAGCT CCTCCCGAGT GGGATTTCTG
    CCCCTGTTGG ACGTGGCAGC TGCTGACTTT GGCCAGACGA ACCAGAAGTT TGGGTGGGTT
    CGCAGTGAAG GGACTGATGT CAGACTACAG AGGGAAGTGG GACTTCCCTG GACTGAGCTG
    CGTACTCTTT CTGCTGCCTC AGCCCAGCTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91062
    Clone ID Related Accession (Same CDS sequence) XM_015472002.1
    Accession Version XM_015472002.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5220bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-3(V) chain isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCGGGAC GCAGGCCGTA GATCCTGTGG ATGTGCTGAA GGCACTGGGC
    ATGCGGGGGG GCCAGGCTGG GATCCCTGAG GGACCCGGCC TCTGCCCCCA GAGGGTCCCG
    GAGGGCGACC GAGCATTCCG GGTGGGCAAG ACTGGCACAC TTGGTGTCCC CACGCAGGAG
    CTCTTCCAAG ATGGGCACTT TCCTGAGAAC TTCTCCATCC TGATCACCCT GCGGGGCCAG
    CCGGCCAACC GTTCTGTCCT TCTGTCCATC TATGATGAGG GCGGCGTCCG GCAGCTCGGC
    TTGGCCTTGG GGCCAGCTCT GGACCTCCTA GGTGACTCCT TCAGCCCCCT CCCCCAGCAG
    GTCAACCTCA CGGATGGCAG GTGGCACCGT GTGGCAGTCA GTGTGGACGG TGGGATGGTG
    ACCCTGGTGG CTGATTGTGA ACCTCAGCTC CCCATGTTGG CTCAGGGACC TCGATTCATC
    AGTACAGCGG GACTCACTGT GCTGGGGACC CAGGACCTTG GCGAGGAGAC TTTTGAGGGA
    GATGTTCAGG AGCTGGTGAT AAGCCCAGAT CCCCAGGCTG CCTTCCAGGC ATGTGAGCGG
    TACCTTCCTG GCTGTGATGA CCTGGATCCA ACAGTCACCA CGGTCCCTCA GGGGGAGCCA
    GAAACCCCTC CCCCTAGGCG GAAGGGGAAA GGAAAAGGGA AGAAAAAAGG GCGAGGTCGT
    AAAGGGAAGG GCAGGAAGAA GAAGAACAAG GAAACTGTGG CTGTGAGTCC TCCTCCTGGC
    TCCCTGGACA ACCAGACCTC CACTGACATC CCCAAGACAG AGACACCAGA ACCAACTCTG
    TCTCCGACAC CCACGCCTTT GGTCATCACC ACAACTGTGA CCTTTGGTAG CAATGTCACC
    ATCCTAGAGG AGGGCCTGGA CCCTGACAAT GGAACTGAAC TGGAGACCCT GGAGACTGAG
    TTAGACAGAG AAGATGAAGA AGAAGGTGAT CCCACCATGG GCCCCCACTT CCGGGCAGCA
    GAACAGTCAT GGAATACTGA GTTCCAGATC TTTCCTGGTG CTGGGGAGAA AGGAGAAAAA
    GGAGAACCAG CTGTGGTCGA ACAGGGACAG CAGTTTGAAG GACCCCCAGG AGCCCCGGGA
    CCCCAAGGGG TGGTTGGCCC CTCAGGCCCT CCTGGCCCCC CAGGATTCCC TGGAGACCGT
    GGTCTACCGG GCCCTGCTGG CCTCCCAGGC ATCCCTGGCA TGGATGGGAT CCGGGGTCTC
    CCAGGCACTG TGATCATGAT GCCGTTCCAG TTTGCAAGAG GCTCTCACAA AGGACCCCCA
    GTATCCTTCC AGCAGGCCCA GGCTCAGGCA GTCCTGCAAC AAGCTCAGCT GTCTATGAAA
    GGCCCTCCTG GCCCGGTGGG GCTCACCGGA CGCCCAGGTC CCGTGGGTCT TCCTGGGTAT
    CCAGGTCTGA AAGGAGAGAT GGGAGAAATG GGGCCGCAGG GTCCCCGAGG GCTTCAGGGA
    CCTCATGGGC CCCCTGGCCG AGAGGGAAAG ATGGGCCGCG CTGGAGCAGA TGGGGCTCGG
    GGCCTCCCCG GCGCTACGGG ACCTAAGGGT GATCGGGGCT TTGATGGCCT GCCAGGGCTG
    CCTGGTGAGA AGGGCCAAAG GGGTGACTTT GGTTATGTGG GGAAACCTGG TCCCCCAGGA
    GAGGATGGAC GGAAGGGAGC AGAGGGACCT CCGGGGCCCA CTGGCCAGGC AGGGGAGCCG
    GGCCCCCGAG GACTGATTGG CCCTAGAGGC TCCCCCGGAC CCCTGGGACG CCCGGGTGTG
    ATTGGAAGTG ATGGTGCTCC AGGTGCCAAA GGAAATGTGG GTCCTCCAGG AGAACCAGGC
    CCCCCAGGAC AGCAGGGAAA TCCTGGGTCC CAGGGAATTC CCGGCCCCCA GGGACCCATC
    GGCACTCCTG GGGAGAAGGG TCCTCCTGGA AACCCAGGAA TTCCAGGCCT CCCAGGATCT
    GAAGGCCCTC CGGGTCACCC AGGTCATGAG GGCCCAACAG GAGAGAAAGG GGCCCAGGGT
    CCAGCAGGGT CAGCAGGCCC TCCGGGCTAT CCAGGACCTC GGGGTGTGAA GGGTACCTCT
    GGCAACCGGG GCCTCCAGGG GGAGAAAGGA GAGAAGGGAG AGGATGGTTT CCCAGGCTTC
    AAGGGTGATG CGGGGCTTAA AGGAGATCGG GGGCAACCCG GACCTCCAGG TCCTCGGGGA
    GAGGATGGTC CGGAGGGGCT AAAGGGGCAG GAGGGGCTGC TTGGCGAGGA GGGTCCCCCA
    GGCTCAGCTG GGGAGAAGGG CAAGCTTGGC GTGCCGGGTC TCCCAGGTTA TCCAGGACGT
    CCAGGCCCTA AGGGATCTAT TGGCTTTCCG GGACCTCTGG GACCATTAGG AGAAAAAGGG
    AAGCGGGGGA AGGCTGGGCA GCCAGGCCTG GAAGGAGAGC GGGGACCACC AGGTGCCCGT
    GGAGAGAGAG GGCAACCAGG TGCCACAGGG CAGCCGGGCC CCAAGGGTGA TGTGGGCCAG
    GACGGAGCCC CTGGGTTCCC TGGAGAAAAG GGCCTCCCAG GTCTGCAAGG TCCTCCTGGG
    TTCTCTGGAC CAAAGGGTCC CCCTGGTCCC CAGGGAAAAG ATGGGGTACC TGGACACCCT
    GGACAGAGAG GAGAATTGGG TTTCCAAGGT CAGACAGGCC CACCTGGACC AGCTGGTGTC
    GTGGGTCCTC AGGGAAAGAC AGGAGAAGCA GGGCCTCTGG GTGAGAGGGG GCCCCCAGGG
    CCCCCTGGAC CTCCTGGTGA ACAAGGTCTT CCGGGCCTGG AAGGCAGAGA GGGGGCCAAG
    GGGGATGTGG GACCACTGGG ACCCCTTGGG AAGGAAGGGC CACCTGGACC CAGAGGTTTC
    CCTGGTCCTC ATGGAGCCCC CGGGGACCCA GGACCGACTG GTTTAAAGGG TGACAAGGGC
    CCCCCAGGTC CTGTTGGTGC CAACGGATCC CCTGGGGAGC GTGGTCCTGT AGGCCCAGCA
    GGGAGCATTG GGCTTCCTGG TCAAAGCGGA GGCCAAGGCC CCGTGGGCCC CACAGGCGAG
    AAGGGTTCCC CGGGTGAACG TGGCGCCCCT GGCCCAACTG GTAAAGATGG GATCCCCGGG
    CCCCTGGGGC TTCCTGGGCC CCCTGGAGCT GCGGGGCCTT CTGGCGAGGA AGGGGACAAG
    GGAGAAGTGG GTGCCCCCGG TCACAAAGGC AGCAAAGGTG ACAAGGGAGA TGCGGGCCCG
    CCTGGACCAA CAGGGATACG GGGTCTGGTG GGACACCCCG GCTCCCCGGG AGCAGACGGG
    GCTCAGGGAC GCCGGGGACC CCCAGGCCTC TTTGGGCAGA AAGGAGATGA TGGAGTGAGA
    GGCTTCCCTG GCATGATTGG TCCTCCTGGC CTACAGGGAA TGCCAGGCCC TGCTGGAGAA
    AAGGGAGAAG TTGGAGATGT AGGGTCCATG GGTCCCCACG GAGCTCCAGG CCCTCGGGGC
    CCCCAGGGCC CCAGCGGATC AGAGGGTCCT CCAGGGTTGC CTGGGGGAGT TGGTCAGCCT
    GGCGCTGTGG GGGAGAAGGG TGAGCCAGGA GAGCCTGGAG ACCCAGGACC CCCCGGGACC
    CCAGGCACCC CGGGCCCCAG GGGAGAAGTT GGTGAAAAGG GGGATACAGG CCCATCTGGG
    GCTGCTGGAC CCCCAGGCAA GAAAGGACCC CCTGGAGAGG ATGGAGCAAA GGGGAATGTG
    GGCCCCACAG GCCTCCCAGG AGACCTAGGC CCCCCCGGAG ACCCTGGAGT TTCGGGTCAA
    GATGGCCCCC CAGGGGAGAA GGGAAACCCT GGAGATGTGG GGGGACCGGG TCCACCTGGC
    GCTTCTGGGG AGCCTGGCGC CCCTGGGCCT CCTGGTAAAA GGGGTCCTTC TGGTCGCATG
    GGTCAAGAAG GTAGAGAAGG AGAGAAAGGT GCCAAGGGGG AACCAGGTCC TGATGGACCC
    CCAGGGAGGA CAGGCCCAGT AGGGGCTCGA GGGCCCCCTG GACGTGTTGG ACCTGAGGGT
    CTTCGTGGGA TCCCTGGACC TGTGGGCGAA CCAGGCCTCC TGGGACCTCC TGGACTGTTA
    GGCCCTCCTG GTCCCCTGGG GCCCTCTGGC CTACCAGGGC TGAAGGGAGA TGCTGGCCCC
    AAGGGAGAAA AGGGCCACAT TGGATTAATT GGTCTTGTCG GCCCCCCTGG GGAGGTTGGT
    GAGAAAGGGG ATCAGGGGTT GCCAGGAGTG CAAGGCTCCC CTGGCCTCCA GGGAGAACCG
    GGTTCTCCTG GCCCCATTGG CTCTCTGGGC CACCCTGGGC CCCAAGGCGT GGTGGGTCCT
    CCAGGACAGA AAGGCTCAAA GGGGTCCCCG GGATCCCAAG GTCCTCGTGG AGACGCTGGC
    CCCCCAGGTC CTCCTGGTCC CCCGGGTCCC TCTGCGGAGC TGCACGGGCT GCGCCGGCGC
    CGACGTTCCC TGCTAGGCTT GGGAACCCCG GAGGGCGGCC TGGAGGAGGT GCTGGCCTCG
    ATGGCGTCGC TGAGCTTCGA GTTAGAGCAG CTGCGGCGCC CTCCCGGCAC GGCTGAGCGC
    CCGGGCCTCG TGTGCAAAGA GTTGCACCGC AGCCATCCGC ATCTGCCAGA TGGGGAATAC
    TGGATTGATC CCAATCAAGG CTGCGCGCGG GATGCCCTCA GGGTTTTCTG TAACTTCACG
    GCAGGAGGAG AAACCTGCCT CTACCCAGAC AAGAAGTTTG AGACCGTGAA ACTGGCCTCT
    TGGTCCAAAG AAAAGCCAGG TGGCTGGTAT AGCACATTCC GTCGAGGGAA AAAGTTCTCC
    TACGTGGATG CCGATGGGGC CGCAGTGAGC GTGGTACAGC TGACCTTCCT GAAGTTGCTG
    AGTGCCACGG CCCGCCAGAG CTTCACATTC ACCTGCCAGA ACTCGGCCGC CTGGCTGGAC
    GAAGCTGCAG GTGGGTTCGC AGTGAAGGGA CTGATGTCAG ACTACAGAGG GAAGTGGGAC
    TTCCCTGGAC TGAGCTGCGT ACTCTTTCTG CTGCCTCAGC CCAGCTCTGA ATACTGTCTC
    CCTCCATGCC TCCCCAAATA CACCTTGCCA TGCCAGGTGG CGTGGGGACC AAACTGGTGG
    GGTTGGGGGG AAACTAGGAT TCTAATGGTG CTGCCCTATT TATACCTGGG TCTATATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327488.1
    CDS153..5372
    Translation

    Target ORF information:

    RefSeq Version XM_015472002.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type V alpha 3 (COL5A3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472002.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
    TTGCAGCTTC TGCCCGGGAC GCAGGCCGTA GATCCTGTGG ATGTGCTGAA GGCACTGGGC
    ATGCGGGGGG GCCAGGCTGG GATCCCTGAG GGACCCGGCC TCTGCCCCCA GAGGGTCCCG
    GAGGGCGACC GAGCATTCCG GGTGGGCAAG ACTGGCACAC TTGGTGTCCC CACGCAGGAG
    CTCTTCCAAG ATGGGCACTT TCCTGAGAAC TTCTCCATCC TGATCACCCT GCGGGGCCAG
    CCGGCCAACC GTTCTGTCCT TCTGTCCATC TATGATGAGG GCGGCGTCCG GCAGCTCGGC
    TTGGCCTTGG GGCCAGCTCT GGACCTCCTA GGTGACTCCT TCAGCCCCCT CCCCCAGCAG
    GTCAACCTCA CGGATGGCAG GTGGCACCGT GTGGCAGTCA GTGTGGACGG TGGGATGGTG
    ACCCTGGTGG CTGATTGTGA ACCTCAGCTC CCCATGTTGG CTCAGGGACC TCGATTCATC
    AGTACAGCGG GACTCACTGT GCTGGGGACC CAGGACCTTG GCGAGGAGAC TTTTGAGGGA
    GATGTTCAGG AGCTGGTGAT AAGCCCAGAT CCCCAGGCTG CCTTCCAGGC ATGTGAGCGG
    TACCTTCCTG GCTGTGATGA CCTGGATCCA ACAGTCACCA CGGTCCCTCA GGGGGAGCCA
    GAAACCCCTC CCCCTAGGCG GAAGGGGAAA GGAAAAGGGA AGAAAAAAGG GCGAGGTCGT
    AAAGGGAAGG GCAGGAAGAA GAAGAACAAG GAAACTGTGG CTGTGAGTCC TCCTCCTGGC
    TCCCTGGACA ACCAGACCTC CACTGACATC CCCAAGACAG AGACACCAGA ACCAACTCTG
    TCTCCGACAC CCACGCCTTT GGTCATCACC ACAACTGTGA CCTTTGGTAG CAATGTCACC
    ATCCTAGAGG AGGGCCTGGA CCCTGACAAT GGAACTGAAC TGGAGACCCT GGAGACTGAG
    TTAGACAGAG AAGATGAAGA AGAAGGTGAT CCCACCATGG GCCCCCACTT CCGGGCAGCA
    GAACAGTCAT GGAATACTGA GTTCCAGATC TTTCCTGGTG CTGGGGAGAA AGGAGAAAAA
    GGAGAACCAG CTGTGGTCGA ACAGGGACAG CAGTTTGAAG GACCCCCAGG AGCCCCGGGA
    CCCCAAGGGG TGGTTGGCCC CTCAGGCCCT CCTGGCCCCC CAGGATTCCC TGGAGACCGT
    GGTCTACCGG GCCCTGCTGG CCTCCCAGGC ATCCCTGGCA TGGATGGGAT CCGGGGTCTC
    CCAGGCACTG TGATCATGAT GCCGTTCCAG TTTGCAAGAG GCTCTCACAA AGGACCCCCA
    GTATCCTTCC AGCAGGCCCA GGCTCAGGCA GTCCTGCAAC AAGCTCAGCT GTCTATGAAA
    GGCCCTCCTG GCCCGGTGGG GCTCACCGGA CGCCCAGGTC CCGTGGGTCT TCCTGGGTAT
    CCAGGTCTGA AAGGAGAGAT GGGAGAAATG GGGCCGCAGG GTCCCCGAGG GCTTCAGGGA
    CCTCATGGGC CCCCTGGCCG AGAGGGAAAG ATGGGCCGCG CTGGAGCAGA TGGGGCTCGG
    GGCCTCCCCG GCGCTACGGG ACCTAAGGGT GATCGGGGCT TTGATGGCCT GCCAGGGCTG
    CCTGGTGAGA AGGGCCAAAG GGGTGACTTT GGTTATGTGG GGAAACCTGG TCCCCCAGGA
    GAGGATGGAC GGAAGGGAGC AGAGGGACCT CCGGGGCCCA CTGGCCAGGC AGGGGAGCCG
    GGCCCCCGAG GACTGATTGG CCCTAGAGGC TCCCCCGGAC CCCTGGGACG CCCGGGTGTG
    ATTGGAAGTG ATGGTGCTCC AGGTGCCAAA GGAAATGTGG GTCCTCCAGG AGAACCAGGC
    CCCCCAGGAC AGCAGGGAAA TCCTGGGTCC CAGGGAATTC CCGGCCCCCA GGGACCCATC
    GGCACTCCTG GGGAGAAGGG TCCTCCTGGA AACCCAGGAA TTCCAGGCCT CCCAGGATCT
    GAAGGCCCTC CGGGTCACCC AGGTCATGAG GGCCCAACAG GAGAGAAAGG GGCCCAGGGT
    CCAGCAGGGT CAGCAGGCCC TCCGGGCTAT CCAGGACCTC GGGGTGTGAA GGGTACCTCT
    GGCAACCGGG GCCTCCAGGG GGAGAAAGGA GAGAAGGGAG AGGATGGTTT CCCAGGCTTC
    AAGGGTGATG CGGGGCTTAA AGGAGATCGG GGGCAACCCG GACCTCCAGG TCCTCGGGGA
    GAGGATGGTC CGGAGGGGCT AAAGGGGCAG GAGGGGCTGC TTGGCGAGGA GGGTCCCCCA
    GGCTCAGCTG GGGAGAAGGG CAAGCTTGGC GTGCCGGGTC TCCCAGGTTA TCCAGGACGT
    CCAGGCCCTA AGGGATCTAT TGGCTTTCCG GGACCTCTGG GACCATTAGG AGAAAAAGGG
    AAGCGGGGGA AGGCTGGGCA GCCAGGCCTG GAAGGAGAGC GGGGACCACC AGGTGCCCGT
    GGAGAGAGAG GGCAACCAGG TGCCACAGGG CAGCCGGGCC CCAAGGGTGA TGTGGGCCAG
    GACGGAGCCC CTGGGTTCCC TGGAGAAAAG GGCCTCCCAG GTCTGCAAGG TCCTCCTGGG
    TTCTCTGGAC CAAAGGGTCC CCCTGGTCCC CAGGGAAAAG ATGGGGTACC TGGACACCCT
    GGACAGAGAG GAGAATTGGG TTTCCAAGGT CAGACAGGCC CACCTGGACC AGCTGGTGTC
    GTGGGTCCTC AGGGAAAGAC AGGAGAAGCA GGGCCTCTGG GTGAGAGGGG GCCCCCAGGG
    CCCCCTGGAC CTCCTGGTGA ACAAGGTCTT CCGGGCCTGG AAGGCAGAGA GGGGGCCAAG
    GGGGATGTGG GACCACTGGG ACCCCTTGGG AAGGAAGGGC CACCTGGACC CAGAGGTTTC
    CCTGGTCCTC ATGGAGCCCC CGGGGACCCA GGACCGACTG GTTTAAAGGG TGACAAGGGC
    CCCCCAGGTC CTGTTGGTGC CAACGGATCC CCTGGGGAGC GTGGTCCTGT AGGCCCAGCA
    GGGAGCATTG GGCTTCCTGG TCAAAGCGGA GGCCAAGGCC CCGTGGGCCC CACAGGCGAG
    AAGGGTTCCC CGGGTGAACG TGGCGCCCCT GGCCCAACTG GTAAAGATGG GATCCCCGGG
    CCCCTGGGGC TTCCTGGGCC CCCTGGAGCT GCGGGGCCTT CTGGCGAGGA AGGGGACAAG
    GGAGAAGTGG GTGCCCCCGG TCACAAAGGC AGCAAAGGTG ACAAGGGAGA TGCGGGCCCG
    CCTGGACCAA CAGGGATACG GGGTCTGGTG GGACACCCCG GCTCCCCGGG AGCAGACGGG
    GCTCAGGGAC GCCGGGGACC CCCAGGCCTC TTTGGGCAGA AAGGAGATGA TGGAGTGAGA
    GGCTTCCCTG GCATGATTGG TCCTCCTGGC CTACAGGGAA TGCCAGGCCC TGCTGGAGAA
    AAGGGAGAAG TTGGAGATGT AGGGTCCATG GGTCCCCACG GAGCTCCAGG CCCTCGGGGC
    CCCCAGGGCC CCAGCGGATC AGAGGGTCCT CCAGGGTTGC CTGGGGGAGT TGGTCAGCCT
    GGCGCTGTGG GGGAGAAGGG TGAGCCAGGA GAGCCTGGAG ACCCAGGACC CCCCGGGACC
    CCAGGCACCC CGGGCCCCAG GGGAGAAGTT GGTGAAAAGG GGGATACAGG CCCATCTGGG
    GCTGCTGGAC CCCCAGGCAA GAAAGGACCC CCTGGAGAGG ATGGAGCAAA GGGGAATGTG
    GGCCCCACAG GCCTCCCAGG AGACCTAGGC CCCCCCGGAG ACCCTGGAGT TTCGGGTCAA
    GATGGCCCCC CAGGGGAGAA GGGAAACCCT GGAGATGTGG GGGGACCGGG TCCACCTGGC
    GCTTCTGGGG AGCCTGGCGC CCCTGGGCCT CCTGGTAAAA GGGGTCCTTC TGGTCGCATG
    GGTCAAGAAG GTAGAGAAGG AGAGAAAGGT GCCAAGGGGG AACCAGGTCC TGATGGACCC
    CCAGGGAGGA CAGGCCCAGT AGGGGCTCGA GGGCCCCCTG GACGTGTTGG ACCTGAGGGT
    CTTCGTGGGA TCCCTGGACC TGTGGGCGAA CCAGGCCTCC TGGGACCTCC TGGACTGTTA
    GGCCCTCCTG GTCCCCTGGG GCCCTCTGGC CTACCAGGGC TGAAGGGAGA TGCTGGCCCC
    AAGGGAGAAA AGGGCCACAT TGGATTAATT GGTCTTGTCG GCCCCCCTGG GGAGGTTGGT
    GAGAAAGGGG ATCAGGGGTT GCCAGGAGTG CAAGGCTCCC CTGGCCTCCA GGGAGAACCG
    GGTTCTCCTG GCCCCATTGG CTCTCTGGGC CACCCTGGGC CCCAAGGCGT GGTGGGTCCT
    CCAGGACAGA AAGGCTCAAA GGGGTCCCCG GGATCCCAAG GTCCTCGTGG AGACGCTGGC
    CCCCCAGGTC CTCCTGGTCC CCCGGGTCCC TCTGCGGAGC TGCACGGGCT GCGCCGGCGC
    CGACGTTCCC TGCTAGGCTT GGGAACCCCG GAGGGCGGCC TGGAGGAGGT GCTGGCCTCG
    ATGGCGTCGC TGAGCTTCGA GTTAGAGCAG CTGCGGCGCC CTCCCGGCAC GGCTGAGCGC
    CCGGGCCTCG TGTGCAAAGA GTTGCACCGC AGCCATCCGC ATCTGCCAGA TGGGGAATAC
    TGGATTGATC CCAATCAAGG CTGCGCGCGG GATGCCCTCA GGGTTTTCTG TAACTTCACG
    GCAGGAGGAG AAACCTGCCT CTACCCAGAC AAGAAGTTTG AGACCGTGAA ACTGGCCTCT
    TGGTCCAAAG AAAAGCCAGG TGGCTGGTAT AGCACATTCC GTCGAGGGAA AAAGTTCTCC
    TACGTGGATG CCGATGGGGC CGCAGTGAGC GTGGTACAGC TGACCTTCCT GAAGTTGCTG
    AGTGCCACGG CCCGCCAGAG CTTCACATTC ACCTGCCAGA ACTCGGCCGC CTGGCTGGAC
    GAAGCTGCAG GTGGGTTCGC AGTGAAGGGA CTGATGTCAG ACTACAGAGG GAAGTGGGAC
    TTCCCTGGAC TGAGCTGCGT ACTCTTTCTG CTGCCTCAGC CCAGCTCTGA ATACTGTCTC
    CCTCCATGCC TCCCCAAATA CACCTTGCCA TGCCAGGTGG CGTGGGGACC AAACTGGTGG
    GGTTGGGGGG AAACTAGGAT TCTAATGGTG CTGCCCTATT TATACCTGGG TCTATATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.