COL5A3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL5A3
Entrez Gene ID	50509
Full Name	collagen type V alpha 3 chain
General protein information	Preferred Names collagen type V alpha 3 chain Names collagen alpha-3(V) chain collagen, type V, alpha 3 pro-(alpha)3(V) collagen
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.2
Summary	This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are thought to be responsible for the symptoms of a subset of patients with Ehlers-Danlos syndrome type III. Messages of several sizes can be detected in northern blots but sequence information cannot confirm the identity of the shorter messages. [provided by RefSeq, Jul 2008].
Disorder MIM:	120216

mRNA and Protein(s)

mRNA	Protein	Name
NM_015719.3	NP_056534.2	collagen alpha-3(V) chain preproprotein
XM_011528042.2	XP_011526344.1	collagen alpha-3(V) chain isoform X1
XM_017026849.2	XP_016882338.1	collagen alpha-3(V) chain isoform X2
NM_015719.4	NP_056534.2	collagen alpha-3(V) chain preproprotein
XM_017026849.1	XP_016882338.1	collagen alpha-3(V) chain isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL5A3	XP_003953413.1
Mus musculus (house mouse)	Col5a3	NP_058615.1
Bos taurus (cattle)	COL5A3	NP_001192553.1
Homo sapiens (human)	COL5A3	NP_056534.2
Rattus norvegicus (Norway rat)	Col5a3	NP_068528.1
	COL5A3	XP_002808215.1
Canis lupus familiaris (dog)	COL5A3	XP_005632967.1

Gene Ontology
Bibliography

Related articles in PubMed

The COL5A3 and MMP9 genes interact in eczema susceptibility.
Margaritte-Jeannin P, Babron MC, Laprise C, Lavielle N, Sarnowski C, Brossard M, Moffatt M, Gagn?-Ouellet V, Etcheto A, Lathrop M, Just J, Cookson WO, Bouzigon E, Demenais F, Dizier MH
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology(2017 Nov)

Proteomic characterization of human multiple myeloma bone marrow extracellular matrix.
Glavey SV, Naba A, Manier S, Clauser K, Tahri S, Park J, Reagan MR, Moschetta M, Mishima Y, Gambella M, Rocci A, Sacco A, O'Dwyer ME, Asara JM, Palumbo A, Roccaro AM, Hynes RO, Ghobrial IM
Leukemia31(11)2426-2434(2017 Nov)

Characterization of the Extracellular Matrix of Normal and Diseased Tissues Using Proteomics.
Naba A, Pearce OMT, Del Rosario A, Ma D, Ding H, Rajeeve V, Cutillas PR, Balkwill FR, Hynes RO
Journal of proteome research16(8)3083-3091(2017 Aug)

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Barallobre-Barreiro J, Gupta SK, Zoccarato A, Kitazume-Taneike R, Fava M, Yin X, Werner T, Hirt MN, Zampetaki A, Viviano A, Chong M, Bern M, Kourliouros A, Domenech N, Willeit P, Shah AM, Jahangiri M, Schaefer L, Fischer JW, Iozzo RV, Viner R, Thum T, Heineke J, Kichler A, Otsu K, Mayr M
Circulation134(11)817-32(2016 Sep)

Type V Collagen in Health, Disease, and Fibrosis.
Mak KM, Png CY, Lee DJ
Anatomical record (Hoboken, N.J. : 2007)299(5)613-29(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Absence of obvious disease-causing mutations and null alleles in a cohort of 13 patients with h-EDS is consistent with exclusion of COL5A3 as a candidate gene for this disease.
Title: Absence of apparent disease causing mutations in COL5A3 in 13 patients with hypermobility Ehlers-Danlos syndrome.

CBF/NF-Y and two repressors regulate the core promoter of the human pro-alpha3(V) collagen gene
Title: The transcription factor CCAAT-binding factor CBF/NF-Y and two repressors regulate the core promoter of the human pro-alpha3(V) collagen gene (COL5A3).

analysis of processing of the Pro-alpha1(V)Pro-alpha2(V)Pro-alpha3(V) procollagen heterotrimer
Title: Biosynthetic processing of the Pro-alpha1(V)Pro-alpha2(V)Pro-alpha3(V) procollagen heterotrimer.

The following COL5A3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL5A3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu07129	NM_015719.4 Latest version!	Homo sapiens collagen type V alpha 3 chain (COL5A3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu67507	XM_011528042.2 Latest version!	Homo sapiens collagen type V alpha 3 chain (COL5A3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu88496	XM_017026849.2 Latest version!	Homo sapiens collagen type V alpha 3 chain (COL5A3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
View Old Accession Versions >>
OHu07129	NM_015719.3	Homo sapiens collagen type V alpha 3 chain (COL5A3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu88496	XM_017026849.1	Homo sapiens collagen type V alpha 3 (COL5A3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL5A3 ( NM_015719.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_056534.2 Homo sapiens collagen type V alpha 3 chain (COL5A3), mRNA.

Price & Availability↑

CloneID	OHu07129
Clone ID Related Accession (Same CDS sequence)	NM_015719.3 , NM_015719.4
Accession Version	NM_015719.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	collagen alpha-3(V) chain preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF177941.1, AC008742.9 and BQ006883.1. This sequence is a reference standard in the RefSeqGene project. On Jul 26, 2006 this sequence version replaced NM_015719.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF177941.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGGAACC GCCGGGACCT GGGCCAGCCG CGGGCCGGTC TCTGCCTGCT CCTGGCCGCG 
CTGCAGCTTC TGCCGGGGAC GCAGGCCGAT CCTGTGGATG TCCTGAAGGC CCTGGGTGTG 
CAGGGAGGCC AGGCTGGGGT CCCCGAGGGG CCTGGCTTCT GTCCCCAGAG GACTCCAGAG 
GGTGACCGGG CATTCAGAAT TGGCCAGGCC AGCACGCTCG GCATCCCCAC GTGGGAACTC 
TTTCCAGAAG GCCACTTTCC TGAGAACTTC TCCTTGCTGA TCACCTTGCG GGGACAGCCA 
GCCAATCAGT CTGTCCTGCT GTCCATTTAT GATGAAAGGG GTGCCCGGCA GTTGGGCCTG 
GCACTGGGGC CAGCGCTGGG TCTCCTAGGT GACCCCTTCC GCCCCCTCCC CCAGCAGGTC 
AACCTCACAG ATGGCAGGTG GCACCGTGTG GCCGTCAGCA TAGATGGTGA GATGGTGACC 
CTGGTAGCTG ACTGTGAAGC TCAGCCCCCT GTTTTGGGCC ATGGCCCCCG CTTCATCAGC 
ATAGCTGGAC TCACTGTGCT GGGGACCCAG GACCTTGGGG AAAAGACTTT CGAGGGAGAC 
ATTCAGGAGC TGCTGATAAG CCCAGATCCT CAGGCTGCCT TCCAGGCTTG TGAGCGGTAC 
CTCCCCGACT GTGACAACCT GGCACCGGCA GCCACAGTGG CTCCCCAGGG TGAACCAGAA 
ACCCCTCGTC CTCGGCGGAA GGGGAAGGGA AAAGGGAGGA AGAAAGGGCG AGGTCGCAAG 
GGGAAGGGCA GGAAAAAGAA CAAGGAAATT TGGACCTCAA GTCCACCTCC TGACTCCGCA 
GAGAACCAGA CCTCCACTGA CATCCCCAAG ACAGAGACTC CAGCTCCAAA TCTGCCTCCG 
ACCCCCACGC CTTTGGTCGT CACCTCCACT GTGACTACTG GACTCAATGC CACGATCCTA 
GAGAGGAGCT TGGACCCTGA CAGTGGAACC GAGCTGGGGA CCCTGGAGAC CAAGGCAGCC 
AGGGAGGATG AAGAAGGAGA TGATTCCACC ATGGGCCCTG ACTTCCGGGC AGCAGAATAT 
CCATCTCGGA CTCAGTTCCA GATCTTTCCT GGTGCTGGAG AGAAAGGAGC AAAAGGAGAG 
CCCGCAGTGA TTGAAAAGGG GCAGCAGTTT GAGGGACCTC CAGGAGCCCC AGGACCCCAA 
GGGGTGGTTG GCCCCTCAGG CCCTCCCGGC CCCCCAGGAT TCCCTGGCGA CCCTGGTCCA 
CCGGGCCCTG CTGGCCTCCC AGGAATCCCC GGCATTGATG GGATCCGAGG CCCACCGGGC 
ACTGTGATCA TGATGCCGTT CCAGTTTGCA GGCGGCTCCT TTAAAGGCCC CCCAGTCTCA 
TTCCAGCAGG CCCAGGCTCA GGCAGTTCTG CAGCAGACTC AGCTCTCTAT GAAAGGCCCC 
CCTGGTCCAG TGGGGCTCAC TGGGCGCCCA GGCCCTGTGG GTCTCCCCGG GCATCCAGGT 
CTGAAAGGAG AGGAGGGAGC AGAAGGGCCA CAGGGTCCCC GAGGCCTGCA GGGACCTCAT 
GGACCCCCTG GCCGAGTGGG CAAGATGGGC CGCCCTGGAG CAGATGGAGC TCGGGGCCTC 
CCAGGGGACA CTGGACCTAA GGGTGATCGT GGCTTCGATG GCCTCCCTGG GCTGCCTGGT 
GAGAAGGGCC AAAGGGGTGA CTTTGGCCAT GTGGGGCAAC CCGGTCCCCC AGGAGAGGAT 
GGTGAGAGGG GAGCAGAGGG ACCTCCAGGG CCCACTGGCC AGGCTGGGGA GCCGGGTCCA 
CGAGGACTGC TTGGCCCCAG AGGCTCTCCT GGCCCCACGG GTCGCCCGGG TGTGACTGGA 
ATTGATGGTG CTCCTGGTGC CAAAGGCAAT GTGGGTCCTC CAGGAGAACC AGGCCCTCCG 
GGACAGCAGG GAAACCATGG GTCCCAGGGA CTCCCCGGTC CCCAGGGACT CATTGGCACT 
CCTGGGGAGA AGGGTCCCCC TGGAAACCCA GGAATTCCAG GCCTCCCAGG ATCCGATGGC 
CCTCTGGGTC ACCCAGGACA TGAGGGCCCC ACGGGAGAGA AAGGGGCTCA GGGTCCACCA 
GGGTCGGCAG GCCCTCCGGG CTATCCTGGA CCTCGGGGAG TGAAGGGCAC TTCAGGCAAC 
CGGGGCCTCC AGGGGGAGAA AGGCGAGAAG GGAGAGGACG GCTTCCCAGG CTTCAAGGGC 
GATGTGGGGC TCAAAGGTGA TCAGGGGAAA CCCGGAGCTC CAGGTCCCCG GGGAGAGGAT 
GGTCCTGAGG GGCCGAAGGG GCAGGCGGGG CAGGCTGGCG AGGAGGGGCC CCCAGGCTCA 
GCTGGGGAGA AGGGCAAGCT TGGGGTGCCA GGCCTCCCAG GTTATCCAGG ACGCCCTGGA 
CCTAAGGGAT CTATTGGATT TCCCGGTCCC CTGGGACCCA TAGGAGAGAA AGGGAAGTCG 
GGAAAGACAG GGCAGCCAGG CCTGGAAGGA GAGCGGGGAC CACCAGGTTC CCGTGGAGAG 
AGGGGGCAAC CGGGTGCCAC AGGGCAACCA GGCCCCAAGG GCGATGTGGG CCAGGATGGA 
GCCCCTGGGA TCCCTGGAGA AAAGGGCCTC CCTGGTCTGC AAGGCCCTCC AGGATTCCCT 
GGGCCAAAGG GCCCCCCTGG TCACCAAGGT AAAGATGGGC GACCAGGGCA CCCTGGACAG 
AGAGGAGAAC TGGGCTTCCA AGGTCAGACA GGCCCGCCTG GACCAGCTGG TGTCTTAGGC 
CCTCAGGGAA AGACAGGAGA AGTGGGACCT CTAGGTGAAA GGGGGCCTCC AGGCCCCCCT 
GGACCTCCTG GTGAACAAGG TCTTCCTGGC CTGGAAGGCA GAGAGGGGGC CAAGGGGGAA 
CTGGGACCAC CAGGACCCCT TGGGAAAGAA GGGCCAGCTG GACTCAGGGG CTTTCCCGGC 
CCCAAAGGGG GCCCTGGGGA CCCGGGACCT ACTGGCTTAA AGGGTGATAA GGGCCCCCCA 
GGGCCCGTGG GGGCCAATGG CTCCCCTGGT GAGCGCGGTC CTTTGGGCCC AGCAGGAGGC 
ATTGGACTTC CTGGCCAAAG TGGCAGCGAA GGCCCCGTTG GCCCTGCAGG CAAGAAGGGG 
TCCCGGGGAG AACGTGGCCC CCCTGGCCCC ACTGGCAAAG ATGGGATCCC AGGGCCCCTG 
GGGCCTCTGG GACCCCCTGG AGCTGCTGGG CCTTCTGGCG AGGAAGGGGA CAAGGGGGAT 
GTGGGTGCCC CCGGACACAA GGGGAGTAAA GGCGATAAAG GAGACGCGGG CCCACCTGGA 
CAACCAGGGA TACGGGGTCC TGCAGGACAC CCAGGTCCCC CGGGAGCAGA CGGGGCTCAG 
GGGCGCCGGG GACCCCCAGG CCTCTTTGGG CAGAAAGGAG ATGACGGAGT CAGAGGCTTT 
GTGGGGGTGA TTGGCCCTCC TGGACTGCAG GGGCTGCCAG GCCCTCCGGG AGAGAAAGGG 
GAGGTCGGAG ACGTCGGGTC CATGGGTCCC CATGGAGCTC CAGGTCCTCG GGGTCCCCAA 
GGCCCCACTG GATCAGAGGG CACTCCAGGG CTGCCTGGAG GAGTTGGTCA GCCAGGCGCC 
GTGGGTGAGA AGGGTGAGCG AGGGGACGCT GGAGACCCAG GGCCTCCAGG AGCCCCAGGC 
ATCCCGGGGC CCAAGGGAGA CATTGGTGAA AAGGGGGACT CAGGCCCATC TGGAGCTGCT 
GGACCCCCAG GCAAGAAAGG TCCCCCTGGA GAGGATGGAG CCAAAGGGAG CGTGGGCCCC 
ACGGGGCTGC CCGGAGATCT AGGGCCCCCA GGAGACCCTG GAGTTTCAGG CATAGATGGT 
TCCCCAGGGG AGAAGGGAGA CCCTGGTGAT GTTGGGGGAC CGGGTCCGCC TGGAGCTTCT 
GGGGAGCCCG GCGCCCCCGG GCCCCCCGGC AAGAGGGGTC CTTCAGGCCA CATGGGTCGA 
GAAGGCAGAG AAGGGGAGAA AGGTGCCAAG GGGGAGCCAG GTCCTGATGG GCCCCCAGGG 
AGGACGGGTC CAATGGGGGC TAGAGGGCCC CCTGGACGTG TGGGGCCTGA GGGTCTTCGA 
GGGATCCCTG GCCCTGTGGG TGAACCAGGC CTCCTGGGAG CCCCTGGACA GATGGGCCCT 
CCTGGCCCCC TGGGGCCCTC TGGCCTCCCA GGGCTGAAGG GAGACACTGG CCCCAAGGGG 
GAAAAGGGCC ACATTGGATT GATCGGTCTC ATTGGCCCCC CGGGAGAAGC TGGTGAGAAA 
GGAGATCAGG GGTTGCCAGG CGTGCAGGGA CCCCCTGGTC CCAAGGGAGA CCCTGGTCCC 
CCTGGTCCCA TTGGCTCTCT GGGCCACCCT GGGCCCCCAG GTGTGGCGGG CCCTCTAGGA 
CAGAAAGGCT CAAAAGGGTC TCCGGGGTCC ATGGGCCCCC GTGGAGACAC TGGACCTGCA 
GGCCCACCAG GCCCCCCGGG TGCCCCTGCC GAGCTGCATG GGCTGCGCAG GCGCCGGCGC 
TTCGTCCCAG TCCCGCTTCC AGTCGTGGAG GGCGGCCTGG AGGAGGTGCT GGCCTCGCTC 
ACATCGCTGA GCTTGGAGCT GGAGCAGCTG CGGCGTCCTC CCGGCACTGC GGAGCGCCCG 
GGCCTCGTGT GCCACGAGCT GCACCGCAAC CACCCGCACC TGCCTGATGG GGAATACTGG 
ATTGACCCCA ACCAGGGCTG CGCGCGGGAC TCGTTCAGGG TTTTTTGCAA CTTCACGGCG 
GGAGGAGAGA CCTGCCTCTA TCCCGACAAG AAGTTTGAGA TCGTGAAATT GGCCTCCTGG 
TCCAAGGAAA AGCCTGGAGG CTGGTATAGC ACATTCCGTC GAGGGAAGAA GTTCTCCTAC 
GTGGACGCCG ACGGGTCCCC AGTGAATGTC GTGCAGCTGA ACTTCCTGAA ACTGCTGAGT 
GCCACAGCTC GCCAGAACTT CACCTACTCC TGCCAGAATG CAGCTGCCTG GCTGGACGAA 
GCCACGGGTG ACTACAGCCA CTCCGCCCGC TTCCTTGGCA CCAATGGAGA GGAGCTGTCT 
TTCAACCAGA CGACAGCAGC CACTGTCAGC GTCCCCCAGG ATGGCTGCCG GCTCCGGAAA 
GGACAGACGA AGACCCTTTT CGAATTCAGC TCTTCTCGAG CGGGATTTCT GCCCCTGTGG 
GATGTGGCGG CCACTGACTT TGGCCAGACG AACCAAAAGT TTGGGTTTGA ACTGGGCCCC 
GTCTGCTTCA GCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056534.2
CDS	87..5324
Translation	MGNRRDLGQPRAGLCLLLAALQLLPGTQADPVDVLKALGVQGGQAGVPEGPGFCPQRTPEGDRAFRIGQASTLGIPTWELFPEGHFPENFSLLITLRGQPANQSVLLSIYDERGARQLGLALGPALGLLGDPFRPLPQQVNLTDGRWHRVAVSIDGEMVTLVADCEAQPPVLGHGPRFISIAGLTVLGTQDLGEKTFEGDIQELLISPDPQAAFQACERYLPDCDNLAPAATVAPQGEPETPRPRRKGKGKGRKKGRGRKGKGRKKNKEIWTSSPPPDSAENQTSTDIPKTETPAPNLPPTPTPLVVTSTVTTGLNATILERSLDPDSGTELGTLETKAAREDEEGDDSTMGPDFRAAEYPSRTQFQIFPGAGEKGAKGEPAVIEKGQQFEGPPGAPGPQGVVGPSGPPGPPGFPGDPGPPGPAGLPGIPGIDGIRGPPGTVIMMPFQFAGGSFKGPPVSFQQAQAQAVLQQTQLSMKGPPGPVGLTGRPGPVGLPGHPGLKGEEGAEGPQGPRGLQGPHGPPGRVGKMGRPGADGARGLPGDTGPKGDRGFDGLPGLPGEKGQRGDFGHVGQPGPPGEDGERGAEGPPGPTGQAGEPGPRGLLGPRGSPGPTGRPGVTGIDGAPGAKGNVGPPGEPGPPGQQGNHGSQGLPGPQGLIGTPGEKGPPGNPGIPGLPGSDGPLGHPGHEGPTGEKGAQGPPGSAGPPGYPGPRGVKGTSGNRGLQGEKGEKGEDGFPGFKGDVGLKGDQGKPGAPGPRGEDGPEGPKGQAGQAGEEGPPGSAGEKGKLGVPGLPGYPGRPGPKGSIGFPGPLGPIGEKGKSGKTGQPGLEGERGPPGSRGERGQPGATGQPGPKGDVGQDGAPGIPGEKGLPGLQGPPGFPGPKGPPGHQGKDGRPGHPGQRGELGFQGQTGPPGPAGVLGPQGKTGEVGPLGERGPPGPPGPPGEQGLPGLEGREGAKGELGPPGPLGKEGPAGLRGFPGPKGGPGDPGPTGLKGDKGPPGPVGANGSPGERGPLGPAGGIGLPGQSGSEGPVGPAGKKGSRGERGPPGPTGKDGIPGPLGPLGPPGAAGPSGEEGDKGDVGAPGHKGSKGDKGDAGPPGQPGIRGPAGHPGPPGADGAQGRRGPPGLFGQKGDDGVRGFVGVIGPPGLQGLPGPPGEKGEVGDVGSMGPHGAPGPRGPQGPTGSEGTPGLPGGVGQPGAVGEKGERGDAGDPGPPGAPGIPGPKGDIGEKGDSGPSGAAGPPGKKGPPGEDGAKGSVGPTGLPGDLGPPGDPGVSGIDGSPGEKGDPGDVGGPGPPGASGEPGAPGPPGKRGPSGHMGREGREGEKGAKGEPGPDGPPGRTGPMGARGPPGRVGPEGLRGIPGPVGEPGLLGAPGQMGPPGPLGPSGLPGLKGDTGPKGEKGHIGLIGLIGPPGEAGEKGDQGLPGVQGPPGPKGDPGPPGPIGSLGHPGPPGVAGPLGQKGSKGSPGSMGPRGDTGPAGPPGPPGAPAELHGLRRRRRFVPVPLPVVEGGLEEVLASLTSLSLELEQLRRPPGTAERPGLVCHELHRNHPHLPDGEYWIDPNQGCARDSFRVFCNFTAGGETCLYPDKKFEIVKLASWSKEKPGGWYSTFRRGKKFSYVDADGSPVNVVQLNFLKLLSATARQNFTYSCQNAAAWLDEATGDYSHSARFLGTNGEELSFNQTTAATVSVPQDGCRLRKGQTKTLFEFSSSRAGFLPLWDVAATDFGQTNQKFGFELGPVCFSS

Target ORF information:

RefSeq Version	NM_015719.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type V alpha 3 chain (COL5A3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015719.3

ORF Insert Sequence:

ATGGGGAACC GCCGGGACCT GGGCCAGCCG CGGGCCGGTC TCTGCCTGCT CCTGGCCGCG 
CTGCAGCTTC TGCCGGGGAC GCAGGCCGAT CCTGTGGATG TCCTGAAGGC CCTGGGTGTG 
CAGGGAGGCC AGGCTGGGGT CCCCGAGGGG CCTGGCTTCT GTCCCCAGAG GACTCCAGAG 
GGTGACCGGG CATTCAGAAT TGGCCAGGCC AGCACGCTCG GCATCCCCAC GTGGGAACTC 
TTTCCAGAAG GCCACTTTCC TGAGAACTTC TCCTTGCTGA TCACCTTGCG GGGACAGCCA 
GCCAATCAGT CTGTCCTGCT GTCCATTTAT GATGAAAGGG GTGCCCGGCA GTTGGGCCTG 
GCACTGGGGC CAGCGCTGGG TCTCCTAGGT GACCCCTTCC GCCCCCTCCC CCAGCAGGTC 
AACCTCACAG ATGGCAGGTG GCACCGTGTG GCCGTCAGCA TAGATGGTGA GATGGTGACC 
CTGGTAGCTG ACTGTGAAGC TCAGCCCCCT GTTTTGGGCC ATGGCCCCCG CTTCATCAGC 
ATAGCTGGAC TCACTGTGCT GGGGACCCAG GACCTTGGGG AAAAGACTTT CGAGGGAGAC 
ATTCAGGAGC TGCTGATAAG CCCAGATCCT CAGGCTGCCT TCCAGGCTTG TGAGCGGTAC 
CTCCCCGACT GTGACAACCT GGCACCGGCA GCCACAGTGG CTCCCCAGGG TGAACCAGAA 
ACCCCTCGTC CTCGGCGGAA GGGGAAGGGA AAAGGGAGGA AGAAAGGGCG AGGTCGCAAG 
GGGAAGGGCA GGAAAAAGAA CAAGGAAATT TGGACCTCAA GTCCACCTCC TGACTCCGCA 
GAGAACCAGA CCTCCACTGA CATCCCCAAG ACAGAGACTC CAGCTCCAAA TCTGCCTCCG 
ACCCCCACGC CTTTGGTCGT CACCTCCACT GTGACTACTG GACTCAATGC CACGATCCTA 
GAGAGGAGCT TGGACCCTGA CAGTGGAACC GAGCTGGGGA CCCTGGAGAC CAAGGCAGCC 
AGGGAGGATG AAGAAGGAGA TGATTCCACC ATGGGCCCTG ACTTCCGGGC AGCAGAATAT 
CCATCTCGGA CTCAGTTCCA GATCTTTCCT GGTGCTGGAG AGAAAGGAGC AAAAGGAGAG 
CCCGCAGTGA TTGAAAAGGG GCAGCAGTTT GAGGGACCTC CAGGAGCCCC AGGACCCCAA 
GGGGTGGTTG GCCCCTCAGG CCCTCCCGGC CCCCCAGGAT TCCCTGGCGA CCCTGGTCCA 
CCGGGCCCTG CTGGCCTCCC AGGAATCCCC GGCATTGATG GGATCCGAGG CCCACCGGGC 
ACTGTGATCA TGATGCCGTT CCAGTTTGCA GGCGGCTCCT TTAAAGGCCC CCCAGTCTCA 
TTCCAGCAGG CCCAGGCTCA GGCAGTTCTG CAGCAGACTC AGCTCTCTAT GAAAGGCCCC 
CCTGGTCCAG TGGGGCTCAC TGGGCGCCCA GGCCCTGTGG GTCTCCCCGG GCATCCAGGT 
CTGAAAGGAG AGGAGGGAGC AGAAGGGCCA CAGGGTCCCC GAGGCCTGCA GGGACCTCAT 
GGACCCCCTG GCCGAGTGGG CAAGATGGGC CGCCCTGGAG CAGATGGAGC TCGGGGCCTC 
CCAGGGGACA CTGGACCTAA GGGTGATCGT GGCTTCGATG GCCTCCCTGG GCTGCCTGGT 
GAGAAGGGCC AAAGGGGTGA CTTTGGCCAT GTGGGGCAAC CCGGTCCCCC AGGAGAGGAT 
GGTGAGAGGG GAGCAGAGGG ACCTCCAGGG CCCACTGGCC AGGCTGGGGA GCCGGGTCCA 
CGAGGACTGC TTGGCCCCAG AGGCTCTCCT GGCCCCACGG GTCGCCCGGG TGTGACTGGA 
ATTGATGGTG CTCCTGGTGC CAAAGGCAAT GTGGGTCCTC CAGGAGAACC AGGCCCTCCG 
GGACAGCAGG GAAACCATGG GTCCCAGGGA CTCCCCGGTC CCCAGGGACT CATTGGCACT 
CCTGGGGAGA AGGGTCCCCC TGGAAACCCA GGAATTCCAG GCCTCCCAGG ATCCGATGGC 
CCTCTGGGTC ACCCAGGACA TGAGGGCCCC ACGGGAGAGA AAGGGGCTCA GGGTCCACCA 
GGGTCGGCAG GCCCTCCGGG CTATCCTGGA CCTCGGGGAG TGAAGGGCAC TTCAGGCAAC 
CGGGGCCTCC AGGGGGAGAA AGGCGAGAAG GGAGAGGACG GCTTCCCAGG CTTCAAGGGC 
GATGTGGGGC TCAAAGGTGA TCAGGGGAAA CCCGGAGCTC CAGGTCCCCG GGGAGAGGAT 
GGTCCTGAGG GGCCGAAGGG GCAGGCGGGG CAGGCTGGCG AGGAGGGGCC CCCAGGCTCA 
GCTGGGGAGA AGGGCAAGCT TGGGGTGCCA GGCCTCCCAG GTTATCCAGG ACGCCCTGGA 
CCTAAGGGAT CTATTGGATT TCCCGGTCCC CTGGGACCCA TAGGAGAGAA AGGGAAGTCG 
GGAAAGACAG GGCAGCCAGG CCTGGAAGGA GAGCGGGGAC CACCAGGTTC CCGTGGAGAG 
AGGGGGCAAC CGGGTGCCAC AGGGCAACCA GGCCCCAAGG GCGATGTGGG CCAGGATGGA 
GCCCCTGGGA TCCCTGGAGA AAAGGGCCTC CCTGGTCTGC AAGGCCCTCC AGGATTCCCT 
GGGCCAAAGG GCCCCCCTGG TCACCAAGGT AAAGATGGGC GACCAGGGCA CCCTGGACAG 
AGAGGAGAAC TGGGCTTCCA AGGTCAGACA GGCCCGCCTG GACCAGCTGG TGTCTTAGGC 
CCTCAGGGAA AGACAGGAGA AGTGGGACCT CTAGGTGAAA GGGGGCCTCC AGGCCCCCCT 
GGACCTCCTG GTGAACAAGG TCTTCCTGGC CTGGAAGGCA GAGAGGGGGC CAAGGGGGAA 
CTGGGACCAC CAGGACCCCT TGGGAAAGAA GGGCCAGCTG GACTCAGGGG CTTTCCCGGC 
CCCAAAGGGG GCCCTGGGGA CCCGGGACCT ACTGGCTTAA AGGGTGATAA GGGCCCCCCA 
GGGCCCGTGG GGGCCAATGG CTCCCCTGGT GAGCGCGGTC CTTTGGGCCC AGCAGGAGGC 
ATTGGACTTC CTGGCCAAAG TGGCAGCGAA GGCCCCGTTG GCCCTGCAGG CAAGAAGGGG 
TCCCGGGGAG AACGTGGCCC CCCTGGCCCC ACTGGCAAAG ATGGGATCCC AGGGCCCCTG 
GGGCCTCTGG GACCCCCTGG AGCTGCTGGG CCTTCTGGCG AGGAAGGGGA CAAGGGGGAT 
GTGGGTGCCC CCGGACACAA GGGGAGTAAA GGCGATAAAG GAGACGCGGG CCCACCTGGA 
CAACCAGGGA TACGGGGTCC TGCAGGACAC CCAGGTCCCC CGGGAGCAGA CGGGGCTCAG 
GGGCGCCGGG GACCCCCAGG CCTCTTTGGG CAGAAAGGAG ATGACGGAGT CAGAGGCTTT 
GTGGGGGTGA TTGGCCCTCC TGGACTGCAG GGGCTGCCAG GCCCTCCGGG AGAGAAAGGG 
GAGGTCGGAG ACGTCGGGTC CATGGGTCCC CATGGAGCTC CAGGTCCTCG GGGTCCCCAA 
GGCCCCACTG GATCAGAGGG CACTCCAGGG CTGCCTGGAG GAGTTGGTCA GCCAGGCGCC 
GTGGGTGAGA AGGGTGAGCG AGGGGACGCT GGAGACCCAG GGCCTCCAGG AGCCCCAGGC 
ATCCCGGGGC CCAAGGGAGA CATTGGTGAA AAGGGGGACT CAGGCCCATC TGGAGCTGCT 
GGACCCCCAG GCAAGAAAGG TCCCCCTGGA GAGGATGGAG CCAAAGGGAG CGTGGGCCCC 
ACGGGGCTGC CCGGAGATCT AGGGCCCCCA GGAGACCCTG GAGTTTCAGG CATAGATGGT 
TCCCCAGGGG AGAAGGGAGA CCCTGGTGAT GTTGGGGGAC CGGGTCCGCC TGGAGCTTCT 
GGGGAGCCCG GCGCCCCCGG GCCCCCCGGC AAGAGGGGTC CTTCAGGCCA CATGGGTCGA 
GAAGGCAGAG AAGGGGAGAA AGGTGCCAAG GGGGAGCCAG GTCCTGATGG GCCCCCAGGG 
AGGACGGGTC CAATGGGGGC TAGAGGGCCC CCTGGACGTG TGGGGCCTGA GGGTCTTCGA 
GGGATCCCTG GCCCTGTGGG TGAACCAGGC CTCCTGGGAG CCCCTGGACA GATGGGCCCT 
CCTGGCCCCC TGGGGCCCTC TGGCCTCCCA GGGCTGAAGG GAGACACTGG CCCCAAGGGG 
GAAAAGGGCC ACATTGGATT GATCGGTCTC ATTGGCCCCC CGGGAGAAGC TGGTGAGAAA 
GGAGATCAGG GGTTGCCAGG CGTGCAGGGA CCCCCTGGTC CCAAGGGAGA CCCTGGTCCC 
CCTGGTCCCA TTGGCTCTCT GGGCCACCCT GGGCCCCCAG GTGTGGCGGG CCCTCTAGGA 
CAGAAAGGCT CAAAAGGGTC TCCGGGGTCC ATGGGCCCCC GTGGAGACAC TGGACCTGCA 
GGCCCACCAG GCCCCCCGGG TGCCCCTGCC GAGCTGCATG GGCTGCGCAG GCGCCGGCGC 
TTCGTCCCAG TCCCGCTTCC AGTCGTGGAG GGCGGCCTGG AGGAGGTGCT GGCCTCGCTC 
ACATCGCTGA GCTTGGAGCT GGAGCAGCTG CGGCGTCCTC CCGGCACTGC GGAGCGCCCG 
GGCCTCGTGT GCCACGAGCT GCACCGCAAC CACCCGCACC TGCCTGATGG GGAATACTGG 
ATTGACCCCA ACCAGGGCTG CGCGCGGGAC TCGTTCAGGG TTTTTTGCAA CTTCACGGCG 
GGAGGAGAGA CCTGCCTCTA TCCCGACAAG AAGTTTGAGA TCGTGAAATT GGCCTCCTGG 
TCCAAGGAAA AGCCTGGAGG CTGGTATAGC ACATTCCGTC GAGGGAAGAA GTTCTCCTAC 
GTGGACGCCG ACGGGTCCCC AGTGAATGTC GTGCAGCTGA ACTTCCTGAA ACTGCTGAGT 
GCCACAGCTC GCCAGAACTT CACCTACTCC TGCCAGAATG CAGCTGCCTG GCTGGACGAA 
GCCACGGGTG ACTACAGCCA CTCCGCCCGC TTCCTTGGCA CCAATGGAGA GGAGCTGTCT 
TTCAACCAGA CGACAGCAGC CACTGTCAGC GTCCCCCAGG ATGGCTGCCG GCTCCGGAAA 
GGACAGACGA AGACCCTTTT CGAATTCAGC TCTTCTCGAG CGGGATTTCT GCCCCTGTGG 
GATGTGGCGG CCACTGACTT TGGCCAGACG AACCAAAAGT TTGGGTTTGA ACTGGGCCCC 
GTCTGCTTCA GCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A3 ( NM_015719.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_056534.2 Homo sapiens collagen type V alpha 3 chain (COL5A3), mRNA.

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CloneID	OHu07129
Clone ID Related Accession (Same CDS sequence)	NM_015719.3 , NM_015719.4
Accession Version	NM_015719.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	collagen alpha-3(V) chain preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008742.9, AF177941.1 and BQ006883.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_015719.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF177941.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000264828.4/ ENSP00000264828.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGAACC GCCGGGACCT GGGCCAGCCG CGGGCCGGTC TCTGCCTGCT CCTGGCCGCG 
CTGCAGCTTC TGCCGGGGAC GCAGGCCGAT CCTGTGGATG TCCTGAAGGC CCTGGGTGTG 
CAGGGAGGCC AGGCTGGGGT CCCCGAGGGG CCTGGCTTCT GTCCCCAGAG GACTCCAGAG 
GGTGACCGGG CATTCAGAAT TGGCCAGGCC AGCACGCTCG GCATCCCCAC GTGGGAACTC 
TTTCCAGAAG GCCACTTTCC TGAGAACTTC TCCTTGCTGA TCACCTTGCG GGGACAGCCA 
GCCAATCAGT CTGTCCTGCT GTCCATTTAT GATGAAAGGG GTGCCCGGCA GTTGGGCCTG 
GCACTGGGGC CAGCGCTGGG TCTCCTAGGT GACCCCTTCC GCCCCCTCCC CCAGCAGGTC 
AACCTCACAG ATGGCAGGTG GCACCGTGTG GCCGTCAGCA TAGATGGTGA GATGGTGACC 
CTGGTAGCTG ACTGTGAAGC TCAGCCCCCT GTTTTGGGCC ATGGCCCCCG CTTCATCAGC 
ATAGCTGGAC TCACTGTGCT GGGGACCCAG GACCTTGGGG AAAAGACTTT CGAGGGAGAC 
ATTCAGGAGC TGCTGATAAG CCCAGATCCT CAGGCTGCCT TCCAGGCTTG TGAGCGGTAC 
CTCCCCGACT GTGACAACCT GGCACCGGCA GCCACAGTGG CTCCCCAGGG TGAACCAGAA 
ACCCCTCGTC CTCGGCGGAA GGGGAAGGGA AAAGGGAGGA AGAAAGGGCG AGGTCGCAAG 
GGGAAGGGCA GGAAAAAGAA CAAGGAAATT TGGACCTCAA GTCCACCTCC TGACTCCGCA 
GAGAACCAGA CCTCCACTGA CATCCCCAAG ACAGAGACTC CAGCTCCAAA TCTGCCTCCG 
ACCCCCACGC CTTTGGTCGT CACCTCCACT GTGACTACTG GACTCAATGC CACGATCCTA 
GAGAGGAGCT TGGACCCTGA CAGTGGAACC GAGCTGGGGA CCCTGGAGAC CAAGGCAGCC 
AGGGAGGATG AAGAAGGAGA TGATTCCACC ATGGGCCCTG ACTTCCGGGC AGCAGAATAT 
CCATCTCGGA CTCAGTTCCA GATCTTTCCT GGTGCTGGAG AGAAAGGAGC AAAAGGAGAG 
CCCGCAGTGA TTGAAAAGGG GCAGCAGTTT GAGGGACCTC CAGGAGCCCC AGGACCCCAA 
GGGGTGGTTG GCCCCTCAGG CCCTCCCGGC CCCCCAGGAT TCCCTGGCGA CCCTGGTCCA 
CCGGGCCCTG CTGGCCTCCC AGGAATCCCC GGCATTGATG GGATCCGAGG CCCACCGGGC 
ACTGTGATCA TGATGCCGTT CCAGTTTGCA GGCGGCTCCT TTAAAGGCCC CCCAGTCTCA 
TTCCAGCAGG CCCAGGCTCA GGCAGTTCTG CAGCAGACTC AGCTCTCTAT GAAAGGCCCC 
CCTGGTCCAG TGGGGCTCAC TGGGCGCCCA GGCCCTGTGG GTCTCCCCGG GCATCCAGGT 
CTGAAAGGAG AGGAGGGAGC AGAAGGGCCA CAGGGTCCCC GAGGCCTGCA GGGACCTCAT 
GGACCCCCTG GCCGAGTGGG CAAGATGGGC CGCCCTGGAG CAGATGGAGC TCGGGGCCTC 
CCAGGGGACA CTGGACCTAA GGGTGATCGT GGCTTCGATG GCCTCCCTGG GCTGCCTGGT 
GAGAAGGGCC AAAGGGGTGA CTTTGGCCAT GTGGGGCAAC CCGGTCCCCC AGGAGAGGAT 
GGTGAGAGGG GAGCAGAGGG ACCTCCAGGG CCCACTGGCC AGGCTGGGGA GCCGGGTCCA 
CGAGGACTGC TTGGCCCCAG AGGCTCTCCT GGCCCCACGG GTCGCCCGGG TGTGACTGGA 
ATTGATGGTG CTCCTGGTGC CAAAGGCAAT GTGGGTCCTC CAGGAGAACC AGGCCCTCCG 
GGACAGCAGG GAAACCATGG GTCCCAGGGA CTCCCCGGTC CCCAGGGACT CATTGGCACT 
CCTGGGGAGA AGGGTCCCCC TGGAAACCCA GGAATTCCAG GCCTCCCAGG ATCCGATGGC 
CCTCTGGGTC ACCCAGGACA TGAGGGCCCC ACGGGAGAGA AAGGGGCTCA GGGTCCACCA 
GGGTCGGCAG GCCCTCCGGG CTATCCTGGA CCTCGGGGAG TGAAGGGCAC TTCAGGCAAC 
CGGGGCCTCC AGGGGGAGAA AGGCGAGAAG GGAGAGGACG GCTTCCCAGG CTTCAAGGGC 
GATGTGGGGC TCAAAGGTGA TCAGGGGAAA CCCGGAGCTC CAGGTCCCCG GGGAGAGGAT 
GGTCCTGAGG GGCCGAAGGG GCAGGCGGGG CAGGCTGGCG AGGAGGGGCC CCCAGGCTCA 
GCTGGGGAGA AGGGCAAGCT TGGGGTGCCA GGCCTCCCAG GTTATCCAGG ACGCCCTGGA 
CCTAAGGGAT CTATTGGATT TCCCGGTCCC CTGGGACCCA TAGGAGAGAA AGGGAAGTCG 
GGAAAGACAG GGCAGCCAGG CCTGGAAGGA GAGCGGGGAC CACCAGGTTC CCGTGGAGAG 
AGGGGGCAAC CGGGTGCCAC AGGGCAACCA GGCCCCAAGG GCGATGTGGG CCAGGATGGA 
GCCCCTGGGA TCCCTGGAGA AAAGGGCCTC CCTGGTCTGC AAGGCCCTCC AGGATTCCCT 
GGGCCAAAGG GCCCCCCTGG TCACCAAGGT AAAGATGGGC GACCAGGGCA CCCTGGACAG 
AGAGGAGAAC TGGGCTTCCA AGGTCAGACA GGCCCGCCTG GACCAGCTGG TGTCTTAGGC 
CCTCAGGGAA AGACAGGAGA AGTGGGACCT CTAGGTGAAA GGGGGCCTCC AGGCCCCCCT 
GGACCTCCTG GTGAACAAGG TCTTCCTGGC CTGGAAGGCA GAGAGGGGGC CAAGGGGGAA 
CTGGGACCAC CAGGACCCCT TGGGAAAGAA GGGCCAGCTG GACTCAGGGG CTTTCCCGGC 
CCCAAAGGGG GCCCTGGGGA CCCGGGACCT ACTGGCTTAA AGGGTGATAA GGGCCCCCCA 
GGGCCCGTGG GGGCCAATGG CTCCCCTGGT GAGCGCGGTC CTTTGGGCCC AGCAGGAGGC 
ATTGGACTTC CTGGCCAAAG TGGCAGCGAA GGCCCCGTTG GCCCTGCAGG CAAGAAGGGG 
TCCCGGGGAG AACGTGGCCC CCCTGGCCCC ACTGGCAAAG ATGGGATCCC AGGGCCCCTG 
GGGCCTCTGG GACCCCCTGG AGCTGCTGGG CCTTCTGGCG AGGAAGGGGA CAAGGGGGAT 
GTGGGTGCCC CCGGACACAA GGGGAGTAAA GGCGATAAAG GAGACGCGGG CCCACCTGGA 
CAACCAGGGA TACGGGGTCC TGCAGGACAC CCAGGTCCCC CGGGAGCAGA CGGGGCTCAG 
GGGCGCCGGG GACCCCCAGG CCTCTTTGGG CAGAAAGGAG ATGACGGAGT CAGAGGCTTT 
GTGGGGGTGA TTGGCCCTCC TGGACTGCAG GGGCTGCCAG GCCCTCCGGG AGAGAAAGGG 
GAGGTCGGAG ACGTCGGGTC CATGGGTCCC CATGGAGCTC CAGGTCCTCG GGGTCCCCAA 
GGCCCCACTG GATCAGAGGG CACTCCAGGG CTGCCTGGAG GAGTTGGTCA GCCAGGCGCC 
GTGGGTGAGA AGGGTGAGCG AGGGGACGCT GGAGACCCAG GGCCTCCAGG AGCCCCAGGC 
ATCCCGGGGC CCAAGGGAGA CATTGGTGAA AAGGGGGACT CAGGCCCATC TGGAGCTGCT 
GGACCCCCAG GCAAGAAAGG TCCCCCTGGA GAGGATGGAG CCAAAGGGAG CGTGGGCCCC 
ACGGGGCTGC CCGGAGATCT AGGGCCCCCA GGAGACCCTG GAGTTTCAGG CATAGATGGT 
TCCCCAGGGG AGAAGGGAGA CCCTGGTGAT GTTGGGGGAC CGGGTCCGCC TGGAGCTTCT 
GGGGAGCCCG GCGCCCCCGG GCCCCCCGGC AAGAGGGGTC CTTCAGGCCA CATGGGTCGA 
GAAGGCAGAG AAGGGGAGAA AGGTGCCAAG GGGGAGCCAG GTCCTGATGG GCCCCCAGGG 
AGGACGGGTC CAATGGGGGC TAGAGGGCCC CCTGGACGTG TGGGGCCTGA GGGTCTTCGA 
GGGATCCCTG GCCCTGTGGG TGAACCAGGC CTCCTGGGAG CCCCTGGACA GATGGGCCCT 
CCTGGCCCCC TGGGGCCCTC TGGCCTCCCA GGGCTGAAGG GAGACACTGG CCCCAAGGGG 
GAAAAGGGCC ACATTGGATT GATCGGTCTC ATTGGCCCCC CGGGAGAAGC TGGTGAGAAA 
GGAGATCAGG GGTTGCCAGG CGTGCAGGGA CCCCCTGGTC CCAAGGGAGA CCCTGGTCCC 
CCTGGTCCCA TTGGCTCTCT GGGCCACCCT GGGCCCCCAG GTGTGGCGGG CCCTCTAGGA 
CAGAAAGGCT CAAAAGGGTC TCCGGGGTCC ATGGGCCCCC GTGGAGACAC TGGACCTGCA 
GGCCCACCAG GCCCCCCGGG TGCCCCTGCC GAGCTGCATG GGCTGCGCAG GCGCCGGCGC 
TTCGTCCCAG TCCCGCTTCC AGTCGTGGAG GGCGGCCTGG AGGAGGTGCT GGCCTCGCTC 
ACATCGCTGA GCTTGGAGCT GGAGCAGCTG CGGCGTCCTC CCGGCACTGC GGAGCGCCCG 
GGCCTCGTGT GCCACGAGCT GCACCGCAAC CACCCGCACC TGCCTGATGG GGAATACTGG 
ATTGACCCCA ACCAGGGCTG CGCGCGGGAC TCGTTCAGGG TTTTTTGCAA CTTCACGGCG 
GGAGGAGAGA CCTGCCTCTA TCCCGACAAG AAGTTTGAGA TCGTGAAATT GGCCTCCTGG 
TCCAAGGAAA AGCCTGGAGG CTGGTATAGC ACATTCCGTC GAGGGAAGAA GTTCTCCTAC 
GTGGACGCCG ACGGGTCCCC AGTGAATGTC GTGCAGCTGA ACTTCCTGAA ACTGCTGAGT 
GCCACAGCTC GCCAGAACTT CACCTACTCC TGCCAGAATG CAGCTGCCTG GCTGGACGAA 
GCCACGGGTG ACTACAGCCA CTCCGCCCGC TTCCTTGGCA CCAATGGAGA GGAGCTGTCT 
TTCAACCAGA CGACAGCAGC CACTGTCAGC GTCCCCCAGG ATGGCTGCCG GCTCCGGAAA 
GGACAGACGA AGACCCTTTT CGAATTCAGC TCTTCTCGAG CGGGATTTCT GCCCCTGTGG 
GATGTGGCGG CCACTGACTT TGGCCAGACG AACCAAAAGT TTGGGTTTGA ACTGGGCCCC 
GTCTGCTTCA GCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056534.2
CDS	120..5357
Translation	MGNRRDLGQPRAGLCLLLAALQLLPGTQADPVDVLKALGVQGGQAGVPEGPGFCPQRTPEGDRAFRIGQASTLGIPTWELFPEGHFPENFSLLITLRGQPANQSVLLSIYDERGARQLGLALGPALGLLGDPFRPLPQQVNLTDGRWHRVAVSIDGEMVTLVADCEAQPPVLGHGPRFISIAGLTVLGTQDLGEKTFEGDIQELLISPDPQAAFQACERYLPDCDNLAPAATVAPQGEPETPRPRRKGKGKGRKKGRGRKGKGRKKNKEIWTSSPPPDSAENQTSTDIPKTETPAPNLPPTPTPLVVTSTVTTGLNATILERSLDPDSGTELGTLETKAAREDEEGDDSTMGPDFRAAEYPSRTQFQIFPGAGEKGAKGEPAVIEKGQQFEGPPGAPGPQGVVGPSGPPGPPGFPGDPGPPGPAGLPGIPGIDGIRGPPGTVIMMPFQFAGGSFKGPPVSFQQAQAQAVLQQTQLSMKGPPGPVGLTGRPGPVGLPGHPGLKGEEGAEGPQGPRGLQGPHGPPGRVGKMGRPGADGARGLPGDTGPKGDRGFDGLPGLPGEKGQRGDFGHVGQPGPPGEDGERGAEGPPGPTGQAGEPGPRGLLGPRGSPGPTGRPGVTGIDGAPGAKGNVGPPGEPGPPGQQGNHGSQGLPGPQGLIGTPGEKGPPGNPGIPGLPGSDGPLGHPGHEGPTGEKGAQGPPGSAGPPGYPGPRGVKGTSGNRGLQGEKGEKGEDGFPGFKGDVGLKGDQGKPGAPGPRGEDGPEGPKGQAGQAGEEGPPGSAGEKGKLGVPGLPGYPGRPGPKGSIGFPGPLGPIGEKGKSGKTGQPGLEGERGPPGSRGERGQPGATGQPGPKGDVGQDGAPGIPGEKGLPGLQGPPGFPGPKGPPGHQGKDGRPGHPGQRGELGFQGQTGPPGPAGVLGPQGKTGEVGPLGERGPPGPPGPPGEQGLPGLEGREGAKGELGPPGPLGKEGPAGLRGFPGPKGGPGDPGPTGLKGDKGPPGPVGANGSPGERGPLGPAGGIGLPGQSGSEGPVGPAGKKGSRGERGPPGPTGKDGIPGPLGPLGPPGAAGPSGEEGDKGDVGAPGHKGSKGDKGDAGPPGQPGIRGPAGHPGPPGADGAQGRRGPPGLFGQKGDDGVRGFVGVIGPPGLQGLPGPPGEKGEVGDVGSMGPHGAPGPRGPQGPTGSEGTPGLPGGVGQPGAVGEKGERGDAGDPGPPGAPGIPGPKGDIGEKGDSGPSGAAGPPGKKGPPGEDGAKGSVGPTGLPGDLGPPGDPGVSGIDGSPGEKGDPGDVGGPGPPGASGEPGAPGPPGKRGPSGHMGREGREGEKGAKGEPGPDGPPGRTGPMGARGPPGRVGPEGLRGIPGPVGEPGLLGAPGQMGPPGPLGPSGLPGLKGDTGPKGEKGHIGLIGLIGPPGEAGEKGDQGLPGVQGPPGPKGDPGPPGPIGSLGHPGPPGVAGPLGQKGSKGSPGSMGPRGDTGPAGPPGPPGAPAELHGLRRRRRFVPVPLPVVEGGLEEVLASLTSLSLELEQLRRPPGTAERPGLVCHELHRNHPHLPDGEYWIDPNQGCARDSFRVFCNFTAGGETCLYPDKKFEIVKLASWSKEKPGGWYSTFRRGKKFSYVDADGSPVNVVQLNFLKLLSATARQNFTYSCQNAAAWLDEATGDYSHSARFLGTNGEELSFNQTTAATVSVPQDGCRLRKGQTKTLFEFSSSRAGFLPLWDVAATDFGQTNQKFGFELGPVCFSS

Target ORF information:

RefSeq Version	NM_015719.4
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type V alpha 3 chain (COL5A3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015719.4

ORF Insert Sequence:

ATGGGGAACC GCCGGGACCT GGGCCAGCCG CGGGCCGGTC TCTGCCTGCT CCTGGCCGCG 
CTGCAGCTTC TGCCGGGGAC GCAGGCCGAT CCTGTGGATG TCCTGAAGGC CCTGGGTGTG 
CAGGGAGGCC AGGCTGGGGT CCCCGAGGGG CCTGGCTTCT GTCCCCAGAG GACTCCAGAG 
GGTGACCGGG CATTCAGAAT TGGCCAGGCC AGCACGCTCG GCATCCCCAC GTGGGAACTC 
TTTCCAGAAG GCCACTTTCC TGAGAACTTC TCCTTGCTGA TCACCTTGCG GGGACAGCCA 
GCCAATCAGT CTGTCCTGCT GTCCATTTAT GATGAAAGGG GTGCCCGGCA GTTGGGCCTG 
GCACTGGGGC CAGCGCTGGG TCTCCTAGGT GACCCCTTCC GCCCCCTCCC CCAGCAGGTC 
AACCTCACAG ATGGCAGGTG GCACCGTGTG GCCGTCAGCA TAGATGGTGA GATGGTGACC 
CTGGTAGCTG ACTGTGAAGC TCAGCCCCCT GTTTTGGGCC ATGGCCCCCG CTTCATCAGC 
ATAGCTGGAC TCACTGTGCT GGGGACCCAG GACCTTGGGG AAAAGACTTT CGAGGGAGAC 
ATTCAGGAGC TGCTGATAAG CCCAGATCCT CAGGCTGCCT TCCAGGCTTG TGAGCGGTAC 
CTCCCCGACT GTGACAACCT GGCACCGGCA GCCACAGTGG CTCCCCAGGG TGAACCAGAA 
ACCCCTCGTC CTCGGCGGAA GGGGAAGGGA AAAGGGAGGA AGAAAGGGCG AGGTCGCAAG 
GGGAAGGGCA GGAAAAAGAA CAAGGAAATT TGGACCTCAA GTCCACCTCC TGACTCCGCA 
GAGAACCAGA CCTCCACTGA CATCCCCAAG ACAGAGACTC CAGCTCCAAA TCTGCCTCCG 
ACCCCCACGC CTTTGGTCGT CACCTCCACT GTGACTACTG GACTCAATGC CACGATCCTA 
GAGAGGAGCT TGGACCCTGA CAGTGGAACC GAGCTGGGGA CCCTGGAGAC CAAGGCAGCC 
AGGGAGGATG AAGAAGGAGA TGATTCCACC ATGGGCCCTG ACTTCCGGGC AGCAGAATAT 
CCATCTCGGA CTCAGTTCCA GATCTTTCCT GGTGCTGGAG AGAAAGGAGC AAAAGGAGAG 
CCCGCAGTGA TTGAAAAGGG GCAGCAGTTT GAGGGACCTC CAGGAGCCCC AGGACCCCAA 
GGGGTGGTTG GCCCCTCAGG CCCTCCCGGC CCCCCAGGAT TCCCTGGCGA CCCTGGTCCA 
CCGGGCCCTG CTGGCCTCCC AGGAATCCCC GGCATTGATG GGATCCGAGG CCCACCGGGC 
ACTGTGATCA TGATGCCGTT CCAGTTTGCA GGCGGCTCCT TTAAAGGCCC CCCAGTCTCA 
TTCCAGCAGG CCCAGGCTCA GGCAGTTCTG CAGCAGACTC AGCTCTCTAT GAAAGGCCCC 
CCTGGTCCAG TGGGGCTCAC TGGGCGCCCA GGCCCTGTGG GTCTCCCCGG GCATCCAGGT 
CTGAAAGGAG AGGAGGGAGC AGAAGGGCCA CAGGGTCCCC GAGGCCTGCA GGGACCTCAT 
GGACCCCCTG GCCGAGTGGG CAAGATGGGC CGCCCTGGAG CAGATGGAGC TCGGGGCCTC 
CCAGGGGACA CTGGACCTAA GGGTGATCGT GGCTTCGATG GCCTCCCTGG GCTGCCTGGT 
GAGAAGGGCC AAAGGGGTGA CTTTGGCCAT GTGGGGCAAC CCGGTCCCCC AGGAGAGGAT 
GGTGAGAGGG GAGCAGAGGG ACCTCCAGGG CCCACTGGCC AGGCTGGGGA GCCGGGTCCA 
CGAGGACTGC TTGGCCCCAG AGGCTCTCCT GGCCCCACGG GTCGCCCGGG TGTGACTGGA 
ATTGATGGTG CTCCTGGTGC CAAAGGCAAT GTGGGTCCTC CAGGAGAACC AGGCCCTCCG 
GGACAGCAGG GAAACCATGG GTCCCAGGGA CTCCCCGGTC CCCAGGGACT CATTGGCACT 
CCTGGGGAGA AGGGTCCCCC TGGAAACCCA GGAATTCCAG GCCTCCCAGG ATCCGATGGC 
CCTCTGGGTC ACCCAGGACA TGAGGGCCCC ACGGGAGAGA AAGGGGCTCA GGGTCCACCA 
GGGTCGGCAG GCCCTCCGGG CTATCCTGGA CCTCGGGGAG TGAAGGGCAC TTCAGGCAAC 
CGGGGCCTCC AGGGGGAGAA AGGCGAGAAG GGAGAGGACG GCTTCCCAGG CTTCAAGGGC 
GATGTGGGGC TCAAAGGTGA TCAGGGGAAA CCCGGAGCTC CAGGTCCCCG GGGAGAGGAT 
GGTCCTGAGG GGCCGAAGGG GCAGGCGGGG CAGGCTGGCG AGGAGGGGCC CCCAGGCTCA 
GCTGGGGAGA AGGGCAAGCT TGGGGTGCCA GGCCTCCCAG GTTATCCAGG ACGCCCTGGA 
CCTAAGGGAT CTATTGGATT TCCCGGTCCC CTGGGACCCA TAGGAGAGAA AGGGAAGTCG 
GGAAAGACAG GGCAGCCAGG CCTGGAAGGA GAGCGGGGAC CACCAGGTTC CCGTGGAGAG 
AGGGGGCAAC CGGGTGCCAC AGGGCAACCA GGCCCCAAGG GCGATGTGGG CCAGGATGGA 
GCCCCTGGGA TCCCTGGAGA AAAGGGCCTC CCTGGTCTGC AAGGCCCTCC AGGATTCCCT 
GGGCCAAAGG GCCCCCCTGG TCACCAAGGT AAAGATGGGC GACCAGGGCA CCCTGGACAG 
AGAGGAGAAC TGGGCTTCCA AGGTCAGACA GGCCCGCCTG GACCAGCTGG TGTCTTAGGC 
CCTCAGGGAA AGACAGGAGA AGTGGGACCT CTAGGTGAAA GGGGGCCTCC AGGCCCCCCT 
GGACCTCCTG GTGAACAAGG TCTTCCTGGC CTGGAAGGCA GAGAGGGGGC CAAGGGGGAA 
CTGGGACCAC CAGGACCCCT TGGGAAAGAA GGGCCAGCTG GACTCAGGGG CTTTCCCGGC 
CCCAAAGGGG GCCCTGGGGA CCCGGGACCT ACTGGCTTAA AGGGTGATAA GGGCCCCCCA 
GGGCCCGTGG GGGCCAATGG CTCCCCTGGT GAGCGCGGTC CTTTGGGCCC AGCAGGAGGC 
ATTGGACTTC CTGGCCAAAG TGGCAGCGAA GGCCCCGTTG GCCCTGCAGG CAAGAAGGGG 
TCCCGGGGAG AACGTGGCCC CCCTGGCCCC ACTGGCAAAG ATGGGATCCC AGGGCCCCTG 
GGGCCTCTGG GACCCCCTGG AGCTGCTGGG CCTTCTGGCG AGGAAGGGGA CAAGGGGGAT 
GTGGGTGCCC CCGGACACAA GGGGAGTAAA GGCGATAAAG GAGACGCGGG CCCACCTGGA 
CAACCAGGGA TACGGGGTCC TGCAGGACAC CCAGGTCCCC CGGGAGCAGA CGGGGCTCAG 
GGGCGCCGGG GACCCCCAGG CCTCTTTGGG CAGAAAGGAG ATGACGGAGT CAGAGGCTTT 
GTGGGGGTGA TTGGCCCTCC TGGACTGCAG GGGCTGCCAG GCCCTCCGGG AGAGAAAGGG 
GAGGTCGGAG ACGTCGGGTC CATGGGTCCC CATGGAGCTC CAGGTCCTCG GGGTCCCCAA 
GGCCCCACTG GATCAGAGGG CACTCCAGGG CTGCCTGGAG GAGTTGGTCA GCCAGGCGCC 
GTGGGTGAGA AGGGTGAGCG AGGGGACGCT GGAGACCCAG GGCCTCCAGG AGCCCCAGGC 
ATCCCGGGGC CCAAGGGAGA CATTGGTGAA AAGGGGGACT CAGGCCCATC TGGAGCTGCT 
GGACCCCCAG GCAAGAAAGG TCCCCCTGGA GAGGATGGAG CCAAAGGGAG CGTGGGCCCC 
ACGGGGCTGC CCGGAGATCT AGGGCCCCCA GGAGACCCTG GAGTTTCAGG CATAGATGGT 
TCCCCAGGGG AGAAGGGAGA CCCTGGTGAT GTTGGGGGAC CGGGTCCGCC TGGAGCTTCT 
GGGGAGCCCG GCGCCCCCGG GCCCCCCGGC AAGAGGGGTC CTTCAGGCCA CATGGGTCGA 
GAAGGCAGAG AAGGGGAGAA AGGTGCCAAG GGGGAGCCAG GTCCTGATGG GCCCCCAGGG 
AGGACGGGTC CAATGGGGGC TAGAGGGCCC CCTGGACGTG TGGGGCCTGA GGGTCTTCGA 
GGGATCCCTG GCCCTGTGGG TGAACCAGGC CTCCTGGGAG CCCCTGGACA GATGGGCCCT 
CCTGGCCCCC TGGGGCCCTC TGGCCTCCCA GGGCTGAAGG GAGACACTGG CCCCAAGGGG 
GAAAAGGGCC ACATTGGATT GATCGGTCTC ATTGGCCCCC CGGGAGAAGC TGGTGAGAAA 
GGAGATCAGG GGTTGCCAGG CGTGCAGGGA CCCCCTGGTC CCAAGGGAGA CCCTGGTCCC 
CCTGGTCCCA TTGGCTCTCT GGGCCACCCT GGGCCCCCAG GTGTGGCGGG CCCTCTAGGA 
CAGAAAGGCT CAAAAGGGTC TCCGGGGTCC ATGGGCCCCC GTGGAGACAC TGGACCTGCA 
GGCCCACCAG GCCCCCCGGG TGCCCCTGCC GAGCTGCATG GGCTGCGCAG GCGCCGGCGC 
TTCGTCCCAG TCCCGCTTCC AGTCGTGGAG GGCGGCCTGG AGGAGGTGCT GGCCTCGCTC 
ACATCGCTGA GCTTGGAGCT GGAGCAGCTG CGGCGTCCTC CCGGCACTGC GGAGCGCCCG 
GGCCTCGTGT GCCACGAGCT GCACCGCAAC CACCCGCACC TGCCTGATGG GGAATACTGG 
ATTGACCCCA ACCAGGGCTG CGCGCGGGAC TCGTTCAGGG TTTTTTGCAA CTTCACGGCG 
GGAGGAGAGA CCTGCCTCTA TCCCGACAAG AAGTTTGAGA TCGTGAAATT GGCCTCCTGG 
TCCAAGGAAA AGCCTGGAGG CTGGTATAGC ACATTCCGTC GAGGGAAGAA GTTCTCCTAC 
GTGGACGCCG ACGGGTCCCC AGTGAATGTC GTGCAGCTGA ACTTCCTGAA ACTGCTGAGT 
GCCACAGCTC GCCAGAACTT CACCTACTCC TGCCAGAATG CAGCTGCCTG GCTGGACGAA 
GCCACGGGTG ACTACAGCCA CTCCGCCCGC TTCCTTGGCA CCAATGGAGA GGAGCTGTCT 
TTCAACCAGA CGACAGCAGC CACTGTCAGC GTCCCCCAGG ATGGCTGCCG GCTCCGGAAA 
GGACAGACGA AGACCCTTTT CGAATTCAGC TCTTCTCGAG CGGGATTTCT GCCCCTGTGG 
GATGTGGCGG CCACTGACTT TGGCCAGACG AACCAAAAGT TTGGGTTTGA ACTGGGCCCC 
GTCTGCTTCA GCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A3 ( XM_011528042.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526344.1 Homo sapiens collagen type V alpha 3 chain (COL5A3), transcript variant X1, mRNA.

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CloneID	OHu67507
Clone ID Related Accession (Same CDS sequence)	XM_011528042.2
Accession Version	XM_011528042.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-3(V) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528042.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAACC GCCGGGACCT GGGCCAGCCG CGGGCCGGTC TCTGCCTGCT CCTGGCCGCG 
CTGCAGCTTC TGCCGGGGAC GCAGGCCGAT CCTGTGGATG TCCTGAAGGC CCTGGGTGTG 
CAGGGAGGCC AGGCTGGGGT CCCCGAGGGG CCTGGCTTCT GTCCCCAGAG GACTCCAGAG 
GGTGACCGGG CATTCAGAAT TGGCCAGGCC AGCACGCTCG GCATCCCCAC GTGGGAACTC 
TTTCCAGGCC ACTTTCCTGA GAACTTCTCC TTGCTGATCA CCTTGCGGGG ACAGCCAGCC 
AATCAGTCTG TCCTGCTGTC CATTTATGAT GAAAGGGGTG CCCGGCAGTT GGGCCTGGCA 
CTGGGGCCAG CGCTGGGTCT CCTAGGTGAC CCCTTCCGCC CCCTCCCCCA GCAGGTCAAC 
CTCACAGATG GCAGGTGGCA CCGTGTGGCC GTCAGCATAG ATGGTGAGAT GGTGACCCTG 
GTAGCTGACT GTGAAGCTCA GCCCCCTGTT TTGGGCCATG GCCCCCGCTT CATCAGCATA 
GCTGGACTCA CTGTGCTGGG GACCCAGGAC CTTGGGGAAA AGACTTTCGA GGGAGACATT 
CAGGAGCTGC TGATAAGCCC AGATCCTCAG GCTGCCTTCC AGGCTTGTGA GCGGTACCTC 
CCCGACTGTG ACAACCTGGC ACCGGCAGCC ACAGTGGCTC CCCAGGGTGA ACCAGAAACC 
CCTCGTCCTC GGCGGAAGGG GAAGGGAAAA GGGAGGAAGA AAGGGCGAGG TCGCAAGGGG 
AAGGGCAGGA AAAAGAACAA GGAAATTTGG ACCTCAAGTC CACCTCCTGA CTCCGCAGAG 
AACCAGACCT CCACTGACAT CCCCAAGACA GAGACTCCAG CTCCAAATCT GCCTCCGACC 
CCCACGCCTT TGGTCGTCAC CTCCACTGTG ACTACTGGAC TCAATGCCAC GATCCTAGAG 
AGGAGCTTGG ACCCTGACAG TGGAACCGAG CTGGGGACCC TGGAGACCAA GGCAGCCAGG 
GAGGATGAAG AAGGAGATGA TTCCACCATG GGCCCTGACT TCCGGGCAGC AGAATATCCA 
TCTCGGACTC AGTTCCAGAT CTTTCCTGGT GCTGGAGAGA AAGGAGCAAA AGGAGAGCCC 
GCAGTGATTG AAAAGGGGCA GCAGTTTGAG GGACCTCCAG GAGCCCCAGG ACCCCAAGGG 
GTGGTTGGCC CCTCAGGCCC TCCCGGCCCC CCAGGATTCC CTGGCGACCC TGGTCCACCG 
GGCCCTGCTG GCCTCCCAGG AATCCCCGGC ATTGATGGGA TCCGAGGCCC ACCGGGCACT 
GTGATCATGA TGCCGTTCCA GTTTGCAGGC GGCTCCTTTA AAGGCCCCCC AGTCTCATTC 
CAGCAGGCCC AGGCTCAGGC AGTTCTGCAG CAGACTCAGC TCTCTATGAA AGGCCCCCCT 
GGTCCAGTGG GGCTCACTGG GCGCCCAGGC CCTGTGGGTC TCCCCGGGCA TCCAGGTCTG 
AAAGGAGAGG AGGGAGCAGA AGGGCCACAG GGTCCCCGAG GCCTGCAGGG ACCTCATGGA 
CCCCCTGGCC GAGTGGGCAA GATGGGCCGC CCTGGAGCAG ATGGAGCTCG GGGCCTCCCA 
GGGGACACTG GACCTAAGGG TGATCGTGGC TTCGATGGCC TCCCTGGGCT GCCTGGTGAG 
AAGGGCCAAA GGGGTGACTT TGGCCATGTG GGGCAACCCG GTCCCCCAGG AGAGGATGGT 
GAGAGGGGAG CAGAGGGACC TCCAGGGCCC ACTGGCCAGG CTGGGGAGCC GGGTCCACGA 
GGACTGCTTG GCCCCAGAGG CTCTCCTGGC CCCACGGGTC GCCCGGGTGT GACTGGAATT 
GATGGTGCTC CTGGTGCCAA AGGCAATGTG GGTCCTCCAG GAGAACCAGG CCCTCCGGGA 
CAGCAGGGAA ACCATGGGTC CCAGGGACTC CCCGGTCCCC AGGGACTCAT TGGCACTCCT 
GGGGAGAAGG GTCCCCCTGG AAACCCAGGA ATTCCAGGCC TCCCAGGATC CGATGGCCCT 
CTGGGTCACC CAGGACATGA GGGCCCCACG GGAGAGAAAG GGGCTCAGGG TCCACCAGGG 
TCGGCAGGCC CTCCGGGCTA TCCTGGACCT CGGGGAGTGA AGGGCACTTC AGGCAACCGG 
GGCCTCCAGG GGGAGAAAGG CGAGAAGGGA GAGGACGGCT TCCCAGGCTT CAAGGGCGAT 
GTGGGGCTCA AAGGTGATCA GGGGAAACCC GGAGCTCCAG GTCCCCGGGG AGAGGATGGT 
CCTGAGGGGC CGAAGGGGCA GGCGGGGCAG GCTGGCGAGG AGGGGCCCCC AGGCTCAGCT 
GGGGAGAAGG GCAAGCTTGG GGTGCCAGGC CTCCCAGGTT ATCCAGGACG CCCTGGACCT 
AAGGGATCTA TTGGATTTCC CGGTCCCCTG GGACCCATAG GAGAGAAAGG GAAGTCGGGA 
AAGACAGGGC AGCCAGGCCT GGAAGGAGAG CGGGGACCAC CAGGTTCCCG TGGAGAGAGG 
GGGCAACCGG GTGCCACAGG GCAACCAGGC CCCAAGGGCG ATGTGGGCCA GGATGGAGCC 
CCTGGGATCC CTGGAGAAAA GGGCCTCCCT GGTCTGCAAG GCCCTCCAGG ATTCCCTGGG 
CCAAAGGGCC CCCCTGGTCA CCAAGGTAAA GATGGGCGAC CAGGGCACCC TGGACAGAGA 
GGAGAACTGG GCTTCCAAGG TCAGACAGGC CCGCCTGGAC CAGCTGGTGT CTTAGGCCCT 
CAGGGAAAGA CAGGAGAAGT GGGACCTCTA GGTGAAAGGG GGCCTCCAGG CCCCCCTGGA 
CCTCCTGGTG AACAAGGTCT TCCTGGCCTG GAAGGCAGAG AGGGGGCCAA GGGGGAACTG 
GGACCACCAG GACCCCTTGG GAAAGAAGGG CCAGCTGGAC TCAGGGGCTT TCCCGGCCCC 
AAAGGGGGCC CTGGGGACCC GGGACCTACT GGCTTAAAGG GTGATAAGGG CCCCCCAGGG 
CCCGTGGGGG CCAATGGCTC CCCTGGTGAG CGCGGTCCTT TGGGCCCAGC AGGAGGCATT 
GGACTTCCTG GCCAAAGTGG CAGCGAAGGC CCCGTTGGCC CTGCAGGCAA GAAGGGGTCC 
CGGGGAGAAC GTGGCCCCCC TGGCCCCACT GGCAAAGATG GGATCCCAGG GCCCCTGGGG 
CCTCTGGGAC CCCCTGGAGC TGCTGGGCCT TCTGGCGAGG AAGGGGACAA GGGGGATGTG 
GGTGCCCCCG GACACAAGGG GAGTAAAGGC GATAAAGGAG ACGCGGGCCC ACCTGGACAA 
CCAGGGATAC GGGGTCCTGC AGGACACCCA GGTCCCCCGG GAGCAGACGG GGCTCAGGGG 
CGCCGGGGAC CCCCAGGCCT CTTTGGGCAG AAAGGAGATG ACGGAGTCAG AGGCTTTGTG 
GGGGTGATTG GCCCTCCTGG ACTGCAGGGG CTGCCAGGCC CTCCGGGAGA GAAAGGGGAG 
GTCGGAGACG TCGGGTCCAT GGGTCCCCAT GGAGCTCCAG GTCCTCGGGG TCCCCAAGGC 
CCCACTGGAT CAGAGGGCAC TCCAGGGCTG CCTGGAGGAG TTGGTCAGCC AGGCGCCGTG 
GGTGAGAAGG GTGAGCGAGG GGACGCTGGA GACCCAGGGC CTCCAGGAGC CCCAGGCATC 
CCGGGGCCCA AGGGAGACAT TGGTGAAAAG GGGGACTCAG GCCCATCTGG AGCTGCTGGA 
CCCCCAGGCA AGAAAGGTCC CCCTGGAGAG GATGGAGCCA AAGGGAGCGT GGGCCCCACG 
GGGCTGCCCG GAGATCTAGG GCCCCCAGGA GACCCTGGAG TTTCAGGCAT AGATGGTTCC 
CCAGGGGAGA AGGGAGACCC TGGTGATGTT GGGGGACCGG GTCCGCCTGG AGCTTCTGGG 
GAGCCCGGCG CCCCCGGGCC CCCCGGCAAG AGGGGTCCTT CAGGCCACAT GGGTCGAGAA 
GGCAGAGAAG GGGAGAAAGG TGCCAAGGGG GAGCCAGGTC CTGATGGGCC CCCAGGGAGG 
ACGGGTCCAA TGGGGGCTAG AGGGCCCCCT GGACGTGTGG GGCCTGAGGG TCTTCGAGGG 
ATCCCTGGCC CTGTGGGTGA ACCAGGCCTC CTGGGAGCCC CTGGACAGAT GGGCCCTCCT 
GGCCCCCTGG GGCCCTCTGG CCTCCCAGGG CTGAAGGGAG ACACTGGCCC CAAGGGGGAA 
AAGGGCCACA TTGGATTGAT CGGTCTCATT GGCCCCCCGG GAGAAGCTGG TGAGAAAGGA 
GATCAGGGGT TGCCAGGCGT GCAGGGACCC CCTGGTCCCA AGGGAGACCC TGGTCCCCCT 
GGTCCCATTG GCTCTCTGGG CCACCCTGGG CCCCCAGGTG TGGCGGGCCC TCTAGGACAG 
AAAGGCTCAA AAGGGTCTCC GGGGTCCATG GGCCCCCGTG GAGACACTGG ACCTGCAGGC 
CCACCAGGCC CCCCGGGTGC CCCTGCCGAG CTGCATGGGC TGCGCAGGCG CCGGCGCTTC 
GTCCCAGTCC CGCTTCCAGT CGTGGAGGGC GGCCTGGAGG AGGTGCTGGC CTCGCTCACA 
TCGCTGAGCT TGGAGCTGGA GCAGCTGCGG CGTCCTCCCG GCACTGCGGA GCGCCCGGGC 
CTCGTGTGCC ACGAGCTGCA CCGCAACCAC CCGCACCTGC CTGATGGGGA ATACTGGATT 
GACCCCAACC AGGGCTGCGC GCGGGACTCG TTCAGGGTTT TTTGCAACTT CACGGCGGGA 
GGAGAGACCT GCCTCTATCC CGACAAGAAG TTTGAGATCG TGAAATTGGC CTCCTGGTCC 
AAGGAAAAGC CTGGAGGCTG GTATAGCACA TTCCGTCGAG GGAAGAAGTT CTCCTACGTG 
GACGCCGACG GGTCCCCAGT GAATGTCGTG CAGCTGAACT TCCTGAAACT GCTGAGTGCC 
ACAGCTCGCC AGAACTTCAC CTACTCCTGC CAGAATGCAG CTGCCTGGCT GGACGAAGCC 
ACGGGTGACT ACAGCCACTC CGCCCGCTTC CTTGGCACCA ATGGAGAGGA GCTGTCTTTC 
AACCAGACGA CAGCAGCCAC TGTCAGCGTC CCCCAGGATG GCTGCCGGCT CCGGAAAGGA 
CAGACGAAGA CCCTTTTCGA ATTCAGCTCT TCTCGAGCGG GATTTCTGCC CCTGTGGGAT 
GTGGCGGCCA CTGACTTTGG CCAGACGAAC CAAAAGTTTG GGTTTGAACT GGGCCCCGTC 
TGCTTCAGCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526344.1
CDS	148..5382
Translation	MGNRRDLGQPRAGLCLLLAALQLLPGTQADPVDVLKALGVQGGQAGVPEGPGFCPQRTPEGDRAFRIGQASTLGIPTWELFPGHFPENFSLLITLRGQPANQSVLLSIYDERGARQLGLALGPALGLLGDPFRPLPQQVNLTDGRWHRVAVSIDGEMVTLVADCEAQPPVLGHGPRFISIAGLTVLGTQDLGEKTFEGDIQELLISPDPQAAFQACERYLPDCDNLAPAATVAPQGEPETPRPRRKGKGKGRKKGRGRKGKGRKKNKEIWTSSPPPDSAENQTSTDIPKTETPAPNLPPTPTPLVVTSTVTTGLNATILERSLDPDSGTELGTLETKAAREDEEGDDSTMGPDFRAAEYPSRTQFQIFPGAGEKGAKGEPAVIEKGQQFEGPPGAPGPQGVVGPSGPPGPPGFPGDPGPPGPAGLPGIPGIDGIRGPPGTVIMMPFQFAGGSFKGPPVSFQQAQAQAVLQQTQLSMKGPPGPVGLTGRPGPVGLPGHPGLKGEEGAEGPQGPRGLQGPHGPPGRVGKMGRPGADGARGLPGDTGPKGDRGFDGLPGLPGEKGQRGDFGHVGQPGPPGEDGERGAEGPPGPTGQAGEPGPRGLLGPRGSPGPTGRPGVTGIDGAPGAKGNVGPPGEPGPPGQQGNHGSQGLPGPQGLIGTPGEKGPPGNPGIPGLPGSDGPLGHPGHEGPTGEKGAQGPPGSAGPPGYPGPRGVKGTSGNRGLQGEKGEKGEDGFPGFKGDVGLKGDQGKPGAPGPRGEDGPEGPKGQAGQAGEEGPPGSAGEKGKLGVPGLPGYPGRPGPKGSIGFPGPLGPIGEKGKSGKTGQPGLEGERGPPGSRGERGQPGATGQPGPKGDVGQDGAPGIPGEKGLPGLQGPPGFPGPKGPPGHQGKDGRPGHPGQRGELGFQGQTGPPGPAGVLGPQGKTGEVGPLGERGPPGPPGPPGEQGLPGLEGREGAKGELGPPGPLGKEGPAGLRGFPGPKGGPGDPGPTGLKGDKGPPGPVGANGSPGERGPLGPAGGIGLPGQSGSEGPVGPAGKKGSRGERGPPGPTGKDGIPGPLGPLGPPGAAGPSGEEGDKGDVGAPGHKGSKGDKGDAGPPGQPGIRGPAGHPGPPGADGAQGRRGPPGLFGQKGDDGVRGFVGVIGPPGLQGLPGPPGEKGEVGDVGSMGPHGAPGPRGPQGPTGSEGTPGLPGGVGQPGAVGEKGERGDAGDPGPPGAPGIPGPKGDIGEKGDSGPSGAAGPPGKKGPPGEDGAKGSVGPTGLPGDLGPPGDPGVSGIDGSPGEKGDPGDVGGPGPPGASGEPGAPGPPGKRGPSGHMGREGREGEKGAKGEPGPDGPPGRTGPMGARGPPGRVGPEGLRGIPGPVGEPGLLGAPGQMGPPGPLGPSGLPGLKGDTGPKGEKGHIGLIGLIGPPGEAGEKGDQGLPGVQGPPGPKGDPGPPGPIGSLGHPGPPGVAGPLGQKGSKGSPGSMGPRGDTGPAGPPGPPGAPAELHGLRRRRRFVPVPLPVVEGGLEEVLASLTSLSLELEQLRRPPGTAERPGLVCHELHRNHPHLPDGEYWIDPNQGCARDSFRVFCNFTAGGETCLYPDKKFEIVKLASWSKEKPGGWYSTFRRGKKFSYVDADGSPVNVVQLNFLKLLSATARQNFTYSCQNAAAWLDEATGDYSHSARFLGTNGEELSFNQTTAATVSVPQDGCRLRKGQTKTLFEFSSSRAGFLPLWDVAATDFGQTNQKFGFELGPVCFSS

Target ORF information:

RefSeq Version	XM_011528042.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type V alpha 3 chain (COL5A3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528042.2

ORF Insert Sequence:

ATGGGGAACC GCCGGGACCT GGGCCAGCCG CGGGCCGGTC TCTGCCTGCT CCTGGCCGCG 
CTGCAGCTTC TGCCGGGGAC GCAGGCCGAT CCTGTGGATG TCCTGAAGGC CCTGGGTGTG 
CAGGGAGGCC AGGCTGGGGT CCCCGAGGGG CCTGGCTTCT GTCCCCAGAG GACTCCAGAG 
GGTGACCGGG CATTCAGAAT TGGCCAGGCC AGCACGCTCG GCATCCCCAC GTGGGAACTC 
TTTCCAGGCC ACTTTCCTGA GAACTTCTCC TTGCTGATCA CCTTGCGGGG ACAGCCAGCC 
AATCAGTCTG TCCTGCTGTC CATTTATGAT GAAAGGGGTG CCCGGCAGTT GGGCCTGGCA 
CTGGGGCCAG CGCTGGGTCT CCTAGGTGAC CCCTTCCGCC CCCTCCCCCA GCAGGTCAAC 
CTCACAGATG GCAGGTGGCA CCGTGTGGCC GTCAGCATAG ATGGTGAGAT GGTGACCCTG 
GTAGCTGACT GTGAAGCTCA GCCCCCTGTT TTGGGCCATG GCCCCCGCTT CATCAGCATA 
GCTGGACTCA CTGTGCTGGG GACCCAGGAC CTTGGGGAAA AGACTTTCGA GGGAGACATT 
CAGGAGCTGC TGATAAGCCC AGATCCTCAG GCTGCCTTCC AGGCTTGTGA GCGGTACCTC 
CCCGACTGTG ACAACCTGGC ACCGGCAGCC ACAGTGGCTC CCCAGGGTGA ACCAGAAACC 
CCTCGTCCTC GGCGGAAGGG GAAGGGAAAA GGGAGGAAGA AAGGGCGAGG TCGCAAGGGG 
AAGGGCAGGA AAAAGAACAA GGAAATTTGG ACCTCAAGTC CACCTCCTGA CTCCGCAGAG 
AACCAGACCT CCACTGACAT CCCCAAGACA GAGACTCCAG CTCCAAATCT GCCTCCGACC 
CCCACGCCTT TGGTCGTCAC CTCCACTGTG ACTACTGGAC TCAATGCCAC GATCCTAGAG 
AGGAGCTTGG ACCCTGACAG TGGAACCGAG CTGGGGACCC TGGAGACCAA GGCAGCCAGG 
GAGGATGAAG AAGGAGATGA TTCCACCATG GGCCCTGACT TCCGGGCAGC AGAATATCCA 
TCTCGGACTC AGTTCCAGAT CTTTCCTGGT GCTGGAGAGA AAGGAGCAAA AGGAGAGCCC 
GCAGTGATTG AAAAGGGGCA GCAGTTTGAG GGACCTCCAG GAGCCCCAGG ACCCCAAGGG 
GTGGTTGGCC CCTCAGGCCC TCCCGGCCCC CCAGGATTCC CTGGCGACCC TGGTCCACCG 
GGCCCTGCTG GCCTCCCAGG AATCCCCGGC ATTGATGGGA TCCGAGGCCC ACCGGGCACT 
GTGATCATGA TGCCGTTCCA GTTTGCAGGC GGCTCCTTTA AAGGCCCCCC AGTCTCATTC 
CAGCAGGCCC AGGCTCAGGC AGTTCTGCAG CAGACTCAGC TCTCTATGAA AGGCCCCCCT 
GGTCCAGTGG GGCTCACTGG GCGCCCAGGC CCTGTGGGTC TCCCCGGGCA TCCAGGTCTG 
AAAGGAGAGG AGGGAGCAGA AGGGCCACAG GGTCCCCGAG GCCTGCAGGG ACCTCATGGA 
CCCCCTGGCC GAGTGGGCAA GATGGGCCGC CCTGGAGCAG ATGGAGCTCG GGGCCTCCCA 
GGGGACACTG GACCTAAGGG TGATCGTGGC TTCGATGGCC TCCCTGGGCT GCCTGGTGAG 
AAGGGCCAAA GGGGTGACTT TGGCCATGTG GGGCAACCCG GTCCCCCAGG AGAGGATGGT 
GAGAGGGGAG CAGAGGGACC TCCAGGGCCC ACTGGCCAGG CTGGGGAGCC GGGTCCACGA 
GGACTGCTTG GCCCCAGAGG CTCTCCTGGC CCCACGGGTC GCCCGGGTGT GACTGGAATT 
GATGGTGCTC CTGGTGCCAA AGGCAATGTG GGTCCTCCAG GAGAACCAGG CCCTCCGGGA 
CAGCAGGGAA ACCATGGGTC CCAGGGACTC CCCGGTCCCC AGGGACTCAT TGGCACTCCT 
GGGGAGAAGG GTCCCCCTGG AAACCCAGGA ATTCCAGGCC TCCCAGGATC CGATGGCCCT 
CTGGGTCACC CAGGACATGA GGGCCCCACG GGAGAGAAAG GGGCTCAGGG TCCACCAGGG 
TCGGCAGGCC CTCCGGGCTA TCCTGGACCT CGGGGAGTGA AGGGCACTTC AGGCAACCGG 
GGCCTCCAGG GGGAGAAAGG CGAGAAGGGA GAGGACGGCT TCCCAGGCTT CAAGGGCGAT 
GTGGGGCTCA AAGGTGATCA GGGGAAACCC GGAGCTCCAG GTCCCCGGGG AGAGGATGGT 
CCTGAGGGGC CGAAGGGGCA GGCGGGGCAG GCTGGCGAGG AGGGGCCCCC AGGCTCAGCT 
GGGGAGAAGG GCAAGCTTGG GGTGCCAGGC CTCCCAGGTT ATCCAGGACG CCCTGGACCT 
AAGGGATCTA TTGGATTTCC CGGTCCCCTG GGACCCATAG GAGAGAAAGG GAAGTCGGGA 
AAGACAGGGC AGCCAGGCCT GGAAGGAGAG CGGGGACCAC CAGGTTCCCG TGGAGAGAGG 
GGGCAACCGG GTGCCACAGG GCAACCAGGC CCCAAGGGCG ATGTGGGCCA GGATGGAGCC 
CCTGGGATCC CTGGAGAAAA GGGCCTCCCT GGTCTGCAAG GCCCTCCAGG ATTCCCTGGG 
CCAAAGGGCC CCCCTGGTCA CCAAGGTAAA GATGGGCGAC CAGGGCACCC TGGACAGAGA 
GGAGAACTGG GCTTCCAAGG TCAGACAGGC CCGCCTGGAC CAGCTGGTGT CTTAGGCCCT 
CAGGGAAAGA CAGGAGAAGT GGGACCTCTA GGTGAAAGGG GGCCTCCAGG CCCCCCTGGA 
CCTCCTGGTG AACAAGGTCT TCCTGGCCTG GAAGGCAGAG AGGGGGCCAA GGGGGAACTG 
GGACCACCAG GACCCCTTGG GAAAGAAGGG CCAGCTGGAC TCAGGGGCTT TCCCGGCCCC 
AAAGGGGGCC CTGGGGACCC GGGACCTACT GGCTTAAAGG GTGATAAGGG CCCCCCAGGG 
CCCGTGGGGG CCAATGGCTC CCCTGGTGAG CGCGGTCCTT TGGGCCCAGC AGGAGGCATT 
GGACTTCCTG GCCAAAGTGG CAGCGAAGGC CCCGTTGGCC CTGCAGGCAA GAAGGGGTCC 
CGGGGAGAAC GTGGCCCCCC TGGCCCCACT GGCAAAGATG GGATCCCAGG GCCCCTGGGG 
CCTCTGGGAC CCCCTGGAGC TGCTGGGCCT TCTGGCGAGG AAGGGGACAA GGGGGATGTG 
GGTGCCCCCG GACACAAGGG GAGTAAAGGC GATAAAGGAG ACGCGGGCCC ACCTGGACAA 
CCAGGGATAC GGGGTCCTGC AGGACACCCA GGTCCCCCGG GAGCAGACGG GGCTCAGGGG 
CGCCGGGGAC CCCCAGGCCT CTTTGGGCAG AAAGGAGATG ACGGAGTCAG AGGCTTTGTG 
GGGGTGATTG GCCCTCCTGG ACTGCAGGGG CTGCCAGGCC CTCCGGGAGA GAAAGGGGAG 
GTCGGAGACG TCGGGTCCAT GGGTCCCCAT GGAGCTCCAG GTCCTCGGGG TCCCCAAGGC 
CCCACTGGAT CAGAGGGCAC TCCAGGGCTG CCTGGAGGAG TTGGTCAGCC AGGCGCCGTG 
GGTGAGAAGG GTGAGCGAGG GGACGCTGGA GACCCAGGGC CTCCAGGAGC CCCAGGCATC 
CCGGGGCCCA AGGGAGACAT TGGTGAAAAG GGGGACTCAG GCCCATCTGG AGCTGCTGGA 
CCCCCAGGCA AGAAAGGTCC CCCTGGAGAG GATGGAGCCA AAGGGAGCGT GGGCCCCACG 
GGGCTGCCCG GAGATCTAGG GCCCCCAGGA GACCCTGGAG TTTCAGGCAT AGATGGTTCC 
CCAGGGGAGA AGGGAGACCC TGGTGATGTT GGGGGACCGG GTCCGCCTGG AGCTTCTGGG 
GAGCCCGGCG CCCCCGGGCC CCCCGGCAAG AGGGGTCCTT CAGGCCACAT GGGTCGAGAA 
GGCAGAGAAG GGGAGAAAGG TGCCAAGGGG GAGCCAGGTC CTGATGGGCC CCCAGGGAGG 
ACGGGTCCAA TGGGGGCTAG AGGGCCCCCT GGACGTGTGG GGCCTGAGGG TCTTCGAGGG 
ATCCCTGGCC CTGTGGGTGA ACCAGGCCTC CTGGGAGCCC CTGGACAGAT GGGCCCTCCT 
GGCCCCCTGG GGCCCTCTGG CCTCCCAGGG CTGAAGGGAG ACACTGGCCC CAAGGGGGAA 
AAGGGCCACA TTGGATTGAT CGGTCTCATT GGCCCCCCGG GAGAAGCTGG TGAGAAAGGA 
GATCAGGGGT TGCCAGGCGT GCAGGGACCC CCTGGTCCCA AGGGAGACCC TGGTCCCCCT 
GGTCCCATTG GCTCTCTGGG CCACCCTGGG CCCCCAGGTG TGGCGGGCCC TCTAGGACAG 
AAAGGCTCAA AAGGGTCTCC GGGGTCCATG GGCCCCCGTG GAGACACTGG ACCTGCAGGC 
CCACCAGGCC CCCCGGGTGC CCCTGCCGAG CTGCATGGGC TGCGCAGGCG CCGGCGCTTC 
GTCCCAGTCC CGCTTCCAGT CGTGGAGGGC GGCCTGGAGG AGGTGCTGGC CTCGCTCACA 
TCGCTGAGCT TGGAGCTGGA GCAGCTGCGG CGTCCTCCCG GCACTGCGGA GCGCCCGGGC 
CTCGTGTGCC ACGAGCTGCA CCGCAACCAC CCGCACCTGC CTGATGGGGA ATACTGGATT 
GACCCCAACC AGGGCTGCGC GCGGGACTCG TTCAGGGTTT TTTGCAACTT CACGGCGGGA 
GGAGAGACCT GCCTCTATCC CGACAAGAAG TTTGAGATCG TGAAATTGGC CTCCTGGTCC 
AAGGAAAAGC CTGGAGGCTG GTATAGCACA TTCCGTCGAG GGAAGAAGTT CTCCTACGTG 
GACGCCGACG GGTCCCCAGT GAATGTCGTG CAGCTGAACT TCCTGAAACT GCTGAGTGCC 
ACAGCTCGCC AGAACTTCAC CTACTCCTGC CAGAATGCAG CTGCCTGGCT GGACGAAGCC 
ACGGGTGACT ACAGCCACTC CGCCCGCTTC CTTGGCACCA ATGGAGAGGA GCTGTCTTTC 
AACCAGACGA CAGCAGCCAC TGTCAGCGTC CCCCAGGATG GCTGCCGGCT CCGGAAAGGA 
CAGACGAAGA CCCTTTTCGA ATTCAGCTCT TCTCGAGCGG GATTTCTGCC CCTGTGGGAT 
GTGGCGGCCA CTGACTTTGG CCAGACGAAC CAAAAGTTTG GGTTTGAACT GGGCCCCGTC 
TGCTTCAGCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A3 ( XM_017026849.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882338.1 Homo sapiens collagen type V alpha 3 chain (COL5A3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu88496
Clone ID Related Accession (Same CDS sequence)	XM_017026849.2 , XM_017026849.1
Accession Version	XM_017026849.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-3(V) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017026849.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGCT CAAAGGGCAA GCTTGGGGTG CCAGGCCTCC CAGGTTATCC AGGACGCCCT 
GGACCTAAGG GATCTATTGG ATTTCCCGGT CCCCTGGGAC CCATAGGAGA GAAAGGGAAG 
TCGGGAAAGA CAGGGCAGCC AGGCCTGGAA GGAGAGCGGG GACCACCAGG TTCCCGTGGA 
GAGAGGGGGC AACCGGGTGC CACAGGGCAA CCAGGCCCCA AGGGCGATGT GGGCCAGGAT 
GGAGCCCCTG GGATCCCTGG AGAAAAGGGC CTCCCTGGTC TGCAAGGCCC TCCAGGATTC 
CCTGGGCCAA AGGGCCCCCC TGGTCACCAA GGTAAAGATG GGCGACCAGG GCACCCTGGA 
CAGAGAGGAG AACTGGGCTT CCAAGGTCAG ACAGGCCCGC CTGGACCAGC TGGTGTCTTA 
GGCCCTCAGG GAAAGACAGG AGAAGTGGGA CCTCTAGGTG AAAGGGGGCC TCCAGGCCCC 
CCTGGACCTC CTGGTGAACA AGGTCTTCCT GGCCTGGAAG GCAGAGAGGG GGCCAAGGGG 
GAACTGGGAC CACCAGGACC CCTTGGGAAA GAAGGGCCAG CTGGACTCAG GGGCTTTCCC 
GGCCCCAAAG GGGGCCCTGG GGACCCGGGA CCTACTGGCT TAAAGGGTGA TAAGGGCCCC 
CCAGGGCCCG TGGGGGCCAA TGGCTCCCCT GGTGAGCGCG GTCCTTTGGG CCCAGCAGGA 
GGCATTGGAC TTCCTGGCCA AAGTGGCAGC GAAGGCCCCG TTGGCCCTGC AGGCAAGAAG 
GGGTCCCGGG GAGAACGTGG CCCCCCTGGC CCCACTGGCA AAGATGGGAT CCCAGGGCCC 
CTGGGGCCTC TGGGACCCCC TGGAGCTGCT GGGCCTTCTG GCGAGGAAGG GGACAAGGGG 
GATGTGGGTG CCCCCGGACA CAAGGGGAGT AAAGGCGATA AAGGAGACGC GGGCCCACCT 
GGACAACCAG GGATACGGGG TCCTGCAGGA CACCCAGGTC CCCCGGGAGC AGACGGGGCT 
CAGGGGCGCC GGGGACCCCC AGGCCTCTTT GGGCAGAAAG GAGATGACGG AGTCAGAGGC 
TTTGTGGGGG TGATTGGCCC TCCTGGACTG CAGGGGCTGC CAGGCCCTCC GGGAGAGAAA 
GGGGAGGTCG GAGACGTCGG GTCCATGGGT CCCCATGGAG CTCCAGGTCC TCGGGGTCCC 
CAAGGCCCCA CTGGATCAGA GGGCACTCCA GGGCTGCCTG GAGGAGTTGG TCAGCCAGGC 
GCCGTGGGTG AGAAGGGTGA GCGAGGGGAC GCTGGAGACC CAGGGCCTCC AGGAGCCCCA 
GGCATCCCGG GGCCCAAGGG AGACATTGGT GAAAAGGGGG ACTCAGGCCC ATCTGGAGCT 
GCTGGACCCC CAGGCAAGAA AGGTCCCCCT GGAGAGGATG GAGCCAAAGG GAGCGTGGGC 
CCCACGGGGC TGCCCGGAGA TCTAGGGCCC CCAGGAGACC CTGGAGTTTC AGGCATAGAT 
GGTTCCCCAG GGGAGAAGGG AGACCCTGGT GATGTTGGGG GACCGGGTCC GCCTGGAGCT 
TCTGGGGAGC CCGGCGCCCC CGGGCCCCCC GGCAAGAGGG GTCCTTCAGG CCACATGGGT 
CGAGAAGGCA GAGAAGGGGA GAAAGGTGCC AAGGGGGAGC CAGGTCCTGA TGGGCCCCCA 
GGGAGGACGG GTCCAATGGG GGCTAGAGGG CCCCCTGGAC GTGTGGGGCC TGAGGGTCTT 
CGAGGGATCC CTGGCCCTGT GGGTGAACCA GGCCTCCTGG GAGCCCCTGG ACAGATGGGC 
CCTCCTGGCC CCCTGGGGCC CTCTGGCCTC CCAGGGCTGA AGGGAGACAC TGGCCCCAAG 
GGGGAAAAGG GCCACATTGG ATTGATCGGT CTCATTGGCC CCCCGGGAGA AGCTGGTGAG 
AAAGGAGATC AGGGGTTGCC AGGCGTGCAG GGACCCCCTG GTCCCAAGGG AGACCCTGGT 
CCCCCTGGTC CCATTGGCTC TCTGGGCCAC CCTGGGCCCC CAGGTGTGGC GGGCCCTCTA 
GGACAGAAAG GCTCAAAAGG GTCTCCGGGG TCCATGGGCC CCCGTGGAGA CACTGGACCT 
GCAGGCCCAC CAGGCCCCCC GGGTGCCCCT GCCGAGCTGC ATGGGCTGCG CAGGCGCCGG 
CGCTTCGTCC CAGTCCCGCT TCCAGTCGTG GAGGGCGGCC TGGAGGAGGT GCTGGCCTCG 
CTCACATCGC TGAGCTTGGA GCTGGAGCAG CTGCGGCGTC CTCCCGGCAC TGCGGAGCGC 
CCGGGCCTCG TGTGCCACGA GCTGCACCGC AACCACCCGC ACCTGCCTGA TGGGGAATAC 
TGGATTGACC CCAACCAGGG CTGCGCGCGG GACTCGTTCA GGGTTTTTTG CAACTTCACG 
GCGGGAGGAG AGACCTGCCT CTATCCCGAC AAGAAGTTTG AGATCGTGAA ATTGGCCTCC 
TGGTCCAAGG AAAAGCCTGG AGGCTGGTAT AGCACATTCC GTCGAGGGAA GAAGTTCTCC 
TACGTGGACG CCGACGGGTC CCCAGTGAAT GTCGTGCAGC TGAACTTCCT GAAACTGCTG 
AGTGCCACAG CTCGCCAGAA CTTCACCTAC TCCTGCCAGA ATGCAGCTGC CTGGCTGGAC 
GAAGCCACGG GTGACTACAG CCACTCCGCC CGCTTCCTTG GCACCAATGG AGAGGAGCTG 
TCTTTCAACC AGACGACAGC AGCCACTGTC AGCGTCCCCC AGGATGGCTG CCGGCTCCGG 
AAAGGACAGA CGAAGACCCT TTTCGAATTC AGCTCTTCTC GAGCGGGATT TCTGCCCCTG 
TGGGATGTGG CGGCCACTGA CTTTGGCCAG ACGAACCAAA AGTTTGGGTT TGAACTGGGC 
CCCGTCTGCT TCAGCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882338.1
CDS	124..3024
Translation	MWGSKGKLGVPGLPGYPGRPGPKGSIGFPGPLGPIGEKGKSGKTGQPGLEGERGPPGSRGERGQPGATGQPGPKGDVGQDGAPGIPGEKGLPGLQGPPGFPGPKGPPGHQGKDGRPGHPGQRGELGFQGQTGPPGPAGVLGPQGKTGEVGPLGERGPPGPPGPPGEQGLPGLEGREGAKGELGPPGPLGKEGPAGLRGFPGPKGGPGDPGPTGLKGDKGPPGPVGANGSPGERGPLGPAGGIGLPGQSGSEGPVGPAGKKGSRGERGPPGPTGKDGIPGPLGPLGPPGAAGPSGEEGDKGDVGAPGHKGSKGDKGDAGPPGQPGIRGPAGHPGPPGADGAQGRRGPPGLFGQKGDDGVRGFVGVIGPPGLQGLPGPPGEKGEVGDVGSMGPHGAPGPRGPQGPTGSEGTPGLPGGVGQPGAVGEKGERGDAGDPGPPGAPGIPGPKGDIGEKGDSGPSGAAGPPGKKGPPGEDGAKGSVGPTGLPGDLGPPGDPGVSGIDGSPGEKGDPGDVGGPGPPGASGEPGAPGPPGKRGPSGHMGREGREGEKGAKGEPGPDGPPGRTGPMGARGPPGRVGPEGLRGIPGPVGEPGLLGAPGQMGPPGPLGPSGLPGLKGDTGPKGEKGHIGLIGLIGPPGEAGEKGDQGLPGVQGPPGPKGDPGPPGPIGSLGHPGPPGVAGPLGQKGSKGSPGSMGPRGDTGPAGPPGPPGAPAELHGLRRRRRFVPVPLPVVEGGLEEVLASLTSLSLELEQLRRPPGTAERPGLVCHELHRNHPHLPDGEYWIDPNQGCARDSFRVFCNFTAGGETCLYPDKKFEIVKLASWSKEKPGGWYSTFRRGKKFSYVDADGSPVNVVQLNFLKLLSATARQNFTYSCQNAAAWLDEATGDYSHSARFLGTNGEELSFNQTTAATVSVPQDGCRLRKGQTKTLFEFSSSRAGFLPLWDVAATDFGQTNQKFGFELGPVCFSS

Target ORF information:

RefSeq Version	XM_017026849.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type V alpha 3 chain (COL5A3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026849.2

ORF Insert Sequence:

ATGTGGGGCT CAAAGGGCAA GCTTGGGGTG CCAGGCCTCC CAGGTTATCC AGGACGCCCT 
GGACCTAAGG GATCTATTGG ATTTCCCGGT CCCCTGGGAC CCATAGGAGA GAAAGGGAAG 
TCGGGAAAGA CAGGGCAGCC AGGCCTGGAA GGAGAGCGGG GACCACCAGG TTCCCGTGGA 
GAGAGGGGGC AACCGGGTGC CACAGGGCAA CCAGGCCCCA AGGGCGATGT GGGCCAGGAT 
GGAGCCCCTG GGATCCCTGG AGAAAAGGGC CTCCCTGGTC TGCAAGGCCC TCCAGGATTC 
CCTGGGCCAA AGGGCCCCCC TGGTCACCAA GGTAAAGATG GGCGACCAGG GCACCCTGGA 
CAGAGAGGAG AACTGGGCTT CCAAGGTCAG ACAGGCCCGC CTGGACCAGC TGGTGTCTTA 
GGCCCTCAGG GAAAGACAGG AGAAGTGGGA CCTCTAGGTG AAAGGGGGCC TCCAGGCCCC 
CCTGGACCTC CTGGTGAACA AGGTCTTCCT GGCCTGGAAG GCAGAGAGGG GGCCAAGGGG 
GAACTGGGAC CACCAGGACC CCTTGGGAAA GAAGGGCCAG CTGGACTCAG GGGCTTTCCC 
GGCCCCAAAG GGGGCCCTGG GGACCCGGGA CCTACTGGCT TAAAGGGTGA TAAGGGCCCC 
CCAGGGCCCG TGGGGGCCAA TGGCTCCCCT GGTGAGCGCG GTCCTTTGGG CCCAGCAGGA 
GGCATTGGAC TTCCTGGCCA AAGTGGCAGC GAAGGCCCCG TTGGCCCTGC AGGCAAGAAG 
GGGTCCCGGG GAGAACGTGG CCCCCCTGGC CCCACTGGCA AAGATGGGAT CCCAGGGCCC 
CTGGGGCCTC TGGGACCCCC TGGAGCTGCT GGGCCTTCTG GCGAGGAAGG GGACAAGGGG 
GATGTGGGTG CCCCCGGACA CAAGGGGAGT AAAGGCGATA AAGGAGACGC GGGCCCACCT 
GGACAACCAG GGATACGGGG TCCTGCAGGA CACCCAGGTC CCCCGGGAGC AGACGGGGCT 
CAGGGGCGCC GGGGACCCCC AGGCCTCTTT GGGCAGAAAG GAGATGACGG AGTCAGAGGC 
TTTGTGGGGG TGATTGGCCC TCCTGGACTG CAGGGGCTGC CAGGCCCTCC GGGAGAGAAA 
GGGGAGGTCG GAGACGTCGG GTCCATGGGT CCCCATGGAG CTCCAGGTCC TCGGGGTCCC 
CAAGGCCCCA CTGGATCAGA GGGCACTCCA GGGCTGCCTG GAGGAGTTGG TCAGCCAGGC 
GCCGTGGGTG AGAAGGGTGA GCGAGGGGAC GCTGGAGACC CAGGGCCTCC AGGAGCCCCA 
GGCATCCCGG GGCCCAAGGG AGACATTGGT GAAAAGGGGG ACTCAGGCCC ATCTGGAGCT 
GCTGGACCCC CAGGCAAGAA AGGTCCCCCT GGAGAGGATG GAGCCAAAGG GAGCGTGGGC 
CCCACGGGGC TGCCCGGAGA TCTAGGGCCC CCAGGAGACC CTGGAGTTTC AGGCATAGAT 
GGTTCCCCAG GGGAGAAGGG AGACCCTGGT GATGTTGGGG GACCGGGTCC GCCTGGAGCT 
TCTGGGGAGC CCGGCGCCCC CGGGCCCCCC GGCAAGAGGG GTCCTTCAGG CCACATGGGT 
CGAGAAGGCA GAGAAGGGGA GAAAGGTGCC AAGGGGGAGC CAGGTCCTGA TGGGCCCCCA 
GGGAGGACGG GTCCAATGGG GGCTAGAGGG CCCCCTGGAC GTGTGGGGCC TGAGGGTCTT 
CGAGGGATCC CTGGCCCTGT GGGTGAACCA GGCCTCCTGG GAGCCCCTGG ACAGATGGGC 
CCTCCTGGCC CCCTGGGGCC CTCTGGCCTC CCAGGGCTGA AGGGAGACAC TGGCCCCAAG 
GGGGAAAAGG GCCACATTGG ATTGATCGGT CTCATTGGCC CCCCGGGAGA AGCTGGTGAG 
AAAGGAGATC AGGGGTTGCC AGGCGTGCAG GGACCCCCTG GTCCCAAGGG AGACCCTGGT 
CCCCCTGGTC CCATTGGCTC TCTGGGCCAC CCTGGGCCCC CAGGTGTGGC GGGCCCTCTA 
GGACAGAAAG GCTCAAAAGG GTCTCCGGGG TCCATGGGCC CCCGTGGAGA CACTGGACCT 
GCAGGCCCAC CAGGCCCCCC GGGTGCCCCT GCCGAGCTGC ATGGGCTGCG CAGGCGCCGG 
CGCTTCGTCC CAGTCCCGCT TCCAGTCGTG GAGGGCGGCC TGGAGGAGGT GCTGGCCTCG 
CTCACATCGC TGAGCTTGGA GCTGGAGCAG CTGCGGCGTC CTCCCGGCAC TGCGGAGCGC 
CCGGGCCTCG TGTGCCACGA GCTGCACCGC AACCACCCGC ACCTGCCTGA TGGGGAATAC 
TGGATTGACC CCAACCAGGG CTGCGCGCGG GACTCGTTCA GGGTTTTTTG CAACTTCACG 
GCGGGAGGAG AGACCTGCCT CTATCCCGAC AAGAAGTTTG AGATCGTGAA ATTGGCCTCC 
TGGTCCAAGG AAAAGCCTGG AGGCTGGTAT AGCACATTCC GTCGAGGGAA GAAGTTCTCC 
TACGTGGACG CCGACGGGTC CCCAGTGAAT GTCGTGCAGC TGAACTTCCT GAAACTGCTG 
AGTGCCACAG CTCGCCAGAA CTTCACCTAC TCCTGCCAGA ATGCAGCTGC CTGGCTGGAC 
GAAGCCACGG GTGACTACAG CCACTCCGCC CGCTTCCTTG GCACCAATGG AGAGGAGCTG 
TCTTTCAACC AGACGACAGC AGCCACTGTC AGCGTCCCCC AGGATGGCTG CCGGCTCCGG 
AAAGGACAGA CGAAGACCCT TTTCGAATTC AGCTCTTCTC GAGCGGGATT TCTGCCCCTG 
TGGGATGTGG CGGCCACTGA CTTTGGCCAG ACGAACCAAA AGTTTGGGTT TGAACTGGGC 
CCCGTCTGCT TCAGCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A3 ( XM_017026849.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882338.1 Homo sapiens collagen type V alpha 3 (COL5A3), transcript variant X2, mRNA.

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CloneID	OHu88496
Clone ID Related Accession (Same CDS sequence)	XM_017026849.2 , XM_017026849.1
Accession Version	XM_017026849.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	collagen alpha-3(V) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGCT CAAAGGGCAA GCTTGGGGTG CCAGGCCTCC CAGGTTATCC AGGACGCCCT 
GGACCTAAGG GATCTATTGG ATTTCCCGGT CCCCTGGGAC CCATAGGAGA GAAAGGGAAG 
TCGGGAAAGA CAGGGCAGCC AGGCCTGGAA GGAGAGCGGG GACCACCAGG TTCCCGTGGA 
GAGAGGGGGC AACCGGGTGC CACAGGGCAA CCAGGCCCCA AGGGCGATGT GGGCCAGGAT 
GGAGCCCCTG GGATCCCTGG AGAAAAGGGC CTCCCTGGTC TGCAAGGCCC TCCAGGATTC 
CCTGGGCCAA AGGGCCCCCC TGGTCACCAA GGTAAAGATG GGCGACCAGG GCACCCTGGA 
CAGAGAGGAG AACTGGGCTT CCAAGGTCAG ACAGGCCCGC CTGGACCAGC TGGTGTCTTA 
GGCCCTCAGG GAAAGACAGG AGAAGTGGGA CCTCTAGGTG AAAGGGGGCC TCCAGGCCCC 
CCTGGACCTC CTGGTGAACA AGGTCTTCCT GGCCTGGAAG GCAGAGAGGG GGCCAAGGGG 
GAACTGGGAC CACCAGGACC CCTTGGGAAA GAAGGGCCAG CTGGACTCAG GGGCTTTCCC 
GGCCCCAAAG GGGGCCCTGG GGACCCGGGA CCTACTGGCT TAAAGGGTGA TAAGGGCCCC 
CCAGGGCCCG TGGGGGCCAA TGGCTCCCCT GGTGAGCGCG GTCCTTTGGG CCCAGCAGGA 
GGCATTGGAC TTCCTGGCCA AAGTGGCAGC GAAGGCCCCG TTGGCCCTGC AGGCAAGAAG 
GGGTCCCGGG GAGAACGTGG CCCCCCTGGC CCCACTGGCA AAGATGGGAT CCCAGGGCCC 
CTGGGGCCTC TGGGACCCCC TGGAGCTGCT GGGCCTTCTG GCGAGGAAGG GGACAAGGGG 
GATGTGGGTG CCCCCGGACA CAAGGGGAGT AAAGGCGATA AAGGAGACGC GGGCCCACCT 
GGACAACCAG GGATACGGGG TCCTGCAGGA CACCCAGGTC CCCCGGGAGC AGACGGGGCT 
CAGGGGCGCC GGGGACCCCC AGGCCTCTTT GGGCAGAAAG GAGATGACGG AGTCAGAGGC 
TTTGTGGGGG TGATTGGCCC TCCTGGACTG CAGGGGCTGC CAGGCCCTCC GGGAGAGAAA 
GGGGAGGTCG GAGACGTCGG GTCCATGGGT CCCCATGGAG CTCCAGGTCC TCGGGGTCCC 
CAAGGCCCCA CTGGATCAGA GGGCACTCCA GGGCTGCCTG GAGGAGTTGG TCAGCCAGGC 
GCCGTGGGTG AGAAGGGTGA GCGAGGGGAC GCTGGAGACC CAGGGCCTCC AGGAGCCCCA 
GGCATCCCGG GGCCCAAGGG AGACATTGGT GAAAAGGGGG ACTCAGGCCC ATCTGGAGCT 
GCTGGACCCC CAGGCAAGAA AGGTCCCCCT GGAGAGGATG GAGCCAAAGG GAGCGTGGGC 
CCCACGGGGC TGCCCGGAGA TCTAGGGCCC CCAGGAGACC CTGGAGTTTC AGGCATAGAT 
GGTTCCCCAG GGGAGAAGGG AGACCCTGGT GATGTTGGGG GACCGGGTCC GCCTGGAGCT 
TCTGGGGAGC CCGGCGCCCC CGGGCCCCCC GGCAAGAGGG GTCCTTCAGG CCACATGGGT 
CGAGAAGGCA GAGAAGGGGA GAAAGGTGCC AAGGGGGAGC CAGGTCCTGA TGGGCCCCCA 
GGGAGGACGG GTCCAATGGG GGCTAGAGGG CCCCCTGGAC GTGTGGGGCC TGAGGGTCTT 
CGAGGGATCC CTGGCCCTGT GGGTGAACCA GGCCTCCTGG GAGCCCCTGG ACAGATGGGC 
CCTCCTGGCC CCCTGGGGCC CTCTGGCCTC CCAGGGCTGA AGGGAGACAC TGGCCCCAAG 
GGGGAAAAGG GCCACATTGG ATTGATCGGT CTCATTGGCC CCCCGGGAGA AGCTGGTGAG 
AAAGGAGATC AGGGGTTGCC AGGCGTGCAG GGACCCCCTG GTCCCAAGGG AGACCCTGGT 
CCCCCTGGTC CCATTGGCTC TCTGGGCCAC CCTGGGCCCC CAGGTGTGGC GGGCCCTCTA 
GGACAGAAAG GCTCAAAAGG GTCTCCGGGG TCCATGGGCC CCCGTGGAGA CACTGGACCT 
GCAGGCCCAC CAGGCCCCCC GGGTGCCCCT GCCGAGCTGC ATGGGCTGCG CAGGCGCCGG 
CGCTTCGTCC CAGTCCCGCT TCCAGTCGTG GAGGGCGGCC TGGAGGAGGT GCTGGCCTCG 
CTCACATCGC TGAGCTTGGA GCTGGAGCAG CTGCGGCGTC CTCCCGGCAC TGCGGAGCGC 
CCGGGCCTCG TGTGCCACGA GCTGCACCGC AACCACCCGC ACCTGCCTGA TGGGGAATAC 
TGGATTGACC CCAACCAGGG CTGCGCGCGG GACTCGTTCA GGGTTTTTTG CAACTTCACG 
GCGGGAGGAG AGACCTGCCT CTATCCCGAC AAGAAGTTTG AGATCGTGAA ATTGGCCTCC 
TGGTCCAAGG AAAAGCCTGG AGGCTGGTAT AGCACATTCC GTCGAGGGAA GAAGTTCTCC 
TACGTGGACG CCGACGGGTC CCCAGTGAAT GTCGTGCAGC TGAACTTCCT GAAACTGCTG 
AGTGCCACAG CTCGCCAGAA CTTCACCTAC TCCTGCCAGA ATGCAGCTGC CTGGCTGGAC 
GAAGCCACGG GTGACTACAG CCACTCCGCC CGCTTCCTTG GCACCAATGG AGAGGAGCTG 
TCTTTCAACC AGACGACAGC AGCCACTGTC AGCGTCCCCC AGGATGGCTG CCGGCTCCGG 
AAAGGACAGA CGAAGACCCT TTTCGAATTC AGCTCTTCTC GAGCGGGATT TCTGCCCCTG 
TGGGATGTGG CGGCCACTGA CTTTGGCCAG ACGAACCAAA AGTTTGGGTT TGAACTGGGC 
CCCGTCTGCT TCAGCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882338.1
CDS	125..3025
Translation	MWGSKGKLGVPGLPGYPGRPGPKGSIGFPGPLGPIGEKGKSGKTGQPGLEGERGPPGSRGERGQPGATGQPGPKGDVGQDGAPGIPGEKGLPGLQGPPGFPGPKGPPGHQGKDGRPGHPGQRGELGFQGQTGPPGPAGVLGPQGKTGEVGPLGERGPPGPPGPPGEQGLPGLEGREGAKGELGPPGPLGKEGPAGLRGFPGPKGGPGDPGPTGLKGDKGPPGPVGANGSPGERGPLGPAGGIGLPGQSGSEGPVGPAGKKGSRGERGPPGPTGKDGIPGPLGPLGPPGAAGPSGEEGDKGDVGAPGHKGSKGDKGDAGPPGQPGIRGPAGHPGPPGADGAQGRRGPPGLFGQKGDDGVRGFVGVIGPPGLQGLPGPPGEKGEVGDVGSMGPHGAPGPRGPQGPTGSEGTPGLPGGVGQPGAVGEKGERGDAGDPGPPGAPGIPGPKGDIGEKGDSGPSGAAGPPGKKGPPGEDGAKGSVGPTGLPGDLGPPGDPGVSGIDGSPGEKGDPGDVGGPGPPGASGEPGAPGPPGKRGPSGHMGREGREGEKGAKGEPGPDGPPGRTGPMGARGPPGRVGPEGLRGIPGPVGEPGLLGAPGQMGPPGPLGPSGLPGLKGDTGPKGEKGHIGLIGLIGPPGEAGEKGDQGLPGVQGPPGPKGDPGPPGPIGSLGHPGPPGVAGPLGQKGSKGSPGSMGPRGDTGPAGPPGPPGAPAELHGLRRRRRFVPVPLPVVEGGLEEVLASLTSLSLELEQLRRPPGTAERPGLVCHELHRNHPHLPDGEYWIDPNQGCARDSFRVFCNFTAGGETCLYPDKKFEIVKLASWSKEKPGGWYSTFRRGKKFSYVDADGSPVNVVQLNFLKLLSATARQNFTYSCQNAAAWLDEATGDYSHSARFLGTNGEELSFNQTTAATVSVPQDGCRLRKGQTKTLFEFSSSRAGFLPLWDVAATDFGQTNQKFGFELGPVCFSS

Target ORF information:

RefSeq Version	XM_017026849.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type V alpha 3 (COL5A3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026849.1

ORF Insert Sequence:

ATGTGGGGCT CAAAGGGCAA GCTTGGGGTG CCAGGCCTCC CAGGTTATCC AGGACGCCCT 
GGACCTAAGG GATCTATTGG ATTTCCCGGT CCCCTGGGAC CCATAGGAGA GAAAGGGAAG 
TCGGGAAAGA CAGGGCAGCC AGGCCTGGAA GGAGAGCGGG GACCACCAGG TTCCCGTGGA 
GAGAGGGGGC AACCGGGTGC CACAGGGCAA CCAGGCCCCA AGGGCGATGT GGGCCAGGAT 
GGAGCCCCTG GGATCCCTGG AGAAAAGGGC CTCCCTGGTC TGCAAGGCCC TCCAGGATTC 
CCTGGGCCAA AGGGCCCCCC TGGTCACCAA GGTAAAGATG GGCGACCAGG GCACCCTGGA 
CAGAGAGGAG AACTGGGCTT CCAAGGTCAG ACAGGCCCGC CTGGACCAGC TGGTGTCTTA 
GGCCCTCAGG GAAAGACAGG AGAAGTGGGA CCTCTAGGTG AAAGGGGGCC TCCAGGCCCC 
CCTGGACCTC CTGGTGAACA AGGTCTTCCT GGCCTGGAAG GCAGAGAGGG GGCCAAGGGG 
GAACTGGGAC CACCAGGACC CCTTGGGAAA GAAGGGCCAG CTGGACTCAG GGGCTTTCCC 
GGCCCCAAAG GGGGCCCTGG GGACCCGGGA CCTACTGGCT TAAAGGGTGA TAAGGGCCCC 
CCAGGGCCCG TGGGGGCCAA TGGCTCCCCT GGTGAGCGCG GTCCTTTGGG CCCAGCAGGA 
GGCATTGGAC TTCCTGGCCA AAGTGGCAGC GAAGGCCCCG TTGGCCCTGC AGGCAAGAAG 
GGGTCCCGGG GAGAACGTGG CCCCCCTGGC CCCACTGGCA AAGATGGGAT CCCAGGGCCC 
CTGGGGCCTC TGGGACCCCC TGGAGCTGCT GGGCCTTCTG GCGAGGAAGG GGACAAGGGG 
GATGTGGGTG CCCCCGGACA CAAGGGGAGT AAAGGCGATA AAGGAGACGC GGGCCCACCT 
GGACAACCAG GGATACGGGG TCCTGCAGGA CACCCAGGTC CCCCGGGAGC AGACGGGGCT 
CAGGGGCGCC GGGGACCCCC AGGCCTCTTT GGGCAGAAAG GAGATGACGG AGTCAGAGGC 
TTTGTGGGGG TGATTGGCCC TCCTGGACTG CAGGGGCTGC CAGGCCCTCC GGGAGAGAAA 
GGGGAGGTCG GAGACGTCGG GTCCATGGGT CCCCATGGAG CTCCAGGTCC TCGGGGTCCC 
CAAGGCCCCA CTGGATCAGA GGGCACTCCA GGGCTGCCTG GAGGAGTTGG TCAGCCAGGC 
GCCGTGGGTG AGAAGGGTGA GCGAGGGGAC GCTGGAGACC CAGGGCCTCC AGGAGCCCCA 
GGCATCCCGG GGCCCAAGGG AGACATTGGT GAAAAGGGGG ACTCAGGCCC ATCTGGAGCT 
GCTGGACCCC CAGGCAAGAA AGGTCCCCCT GGAGAGGATG GAGCCAAAGG GAGCGTGGGC 
CCCACGGGGC TGCCCGGAGA TCTAGGGCCC CCAGGAGACC CTGGAGTTTC AGGCATAGAT 
GGTTCCCCAG GGGAGAAGGG AGACCCTGGT GATGTTGGGG GACCGGGTCC GCCTGGAGCT 
TCTGGGGAGC CCGGCGCCCC CGGGCCCCCC GGCAAGAGGG GTCCTTCAGG CCACATGGGT 
CGAGAAGGCA GAGAAGGGGA GAAAGGTGCC AAGGGGGAGC CAGGTCCTGA TGGGCCCCCA 
GGGAGGACGG GTCCAATGGG GGCTAGAGGG CCCCCTGGAC GTGTGGGGCC TGAGGGTCTT 
CGAGGGATCC CTGGCCCTGT GGGTGAACCA GGCCTCCTGG GAGCCCCTGG ACAGATGGGC 
CCTCCTGGCC CCCTGGGGCC CTCTGGCCTC CCAGGGCTGA AGGGAGACAC TGGCCCCAAG 
GGGGAAAAGG GCCACATTGG ATTGATCGGT CTCATTGGCC CCCCGGGAGA AGCTGGTGAG 
AAAGGAGATC AGGGGTTGCC AGGCGTGCAG GGACCCCCTG GTCCCAAGGG AGACCCTGGT 
CCCCCTGGTC CCATTGGCTC TCTGGGCCAC CCTGGGCCCC CAGGTGTGGC GGGCCCTCTA 
GGACAGAAAG GCTCAAAAGG GTCTCCGGGG TCCATGGGCC CCCGTGGAGA CACTGGACCT 
GCAGGCCCAC CAGGCCCCCC GGGTGCCCCT GCCGAGCTGC ATGGGCTGCG CAGGCGCCGG 
CGCTTCGTCC CAGTCCCGCT TCCAGTCGTG GAGGGCGGCC TGGAGGAGGT GCTGGCCTCG 
CTCACATCGC TGAGCTTGGA GCTGGAGCAG CTGCGGCGTC CTCCCGGCAC TGCGGAGCGC 
CCGGGCCTCG TGTGCCACGA GCTGCACCGC AACCACCCGC ACCTGCCTGA TGGGGAATAC 
TGGATTGACC CCAACCAGGG CTGCGCGCGG GACTCGTTCA GGGTTTTTTG CAACTTCACG 
GCGGGAGGAG AGACCTGCCT CTATCCCGAC AAGAAGTTTG AGATCGTGAA ATTGGCCTCC 
TGGTCCAAGG AAAAGCCTGG AGGCTGGTAT AGCACATTCC GTCGAGGGAA GAAGTTCTCC 
TACGTGGACG CCGACGGGTC CCCAGTGAAT GTCGTGCAGC TGAACTTCCT GAAACTGCTG 
AGTGCCACAG CTCGCCAGAA CTTCACCTAC TCCTGCCAGA ATGCAGCTGC CTGGCTGGAC 
GAAGCCACGG GTGACTACAG CCACTCCGCC CGCTTCCTTG GCACCAATGG AGAGGAGCTG 
TCTTTCAACC AGACGACAGC AGCCACTGTC AGCGTCCCCC AGGATGGCTG CCGGCTCCGG 
AAAGGACAGA CGAAGACCCT TTTCGAATTC AGCTCTTCTC GAGCGGGATT TCTGCCCCTG 
TGGGATGTGG CGGCCACTGA CTTTGGCCAG ACGAACCAAA AGTTTGGGTT TGAACTGGGC 
CCCGTCTGCT TCAGCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The COL5A3 and MMP9 genes interact in eczema susceptibility.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology(2017 Nov)
Margaritte-Jeannin P,Babron MC,Laprise C,Lavielle N,Sarnowski C,Brossard M,Moffatt M,Gagn?-Ouellet V,Etcheto A,Lathrop M,Just J,Cookson WO,Bouzigon E,Demenais F,Dizier MH

Proteomic characterization of human multiple myeloma bone marrow extracellular matrix.
Leukemia31(11)2426-2434(2017 Nov)
Glavey SV,Naba A,Manier S,Clauser K,Tahri S,Park J,Reagan MR,Moschetta M,Mishima Y,Gambella M,Rocci A,Sacco A,O'Dwyer ME,Asara JM,Palumbo A,Roccaro AM,Hynes RO,Ghobrial IM

Characterization of the Extracellular Matrix of Normal and Diseased Tissues Using Proteomics.
Journal of proteome research16(8)3083-3091(2017 Aug)
Naba A,Pearce OMT,Del Rosario A,Ma D,Ding H,Rajeeve V,Cutillas PR,Balkwill FR,Hynes RO

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Circulation134(11)817-32(2016 Sep)
Barallobre-Barreiro J,Gupta SK,Zoccarato A,Kitazume-Taneike R,Fava M,Yin X,Werner T,Hirt MN,Zampetaki A,Viviano A,Chong M,Bern M,Kourliouros A,Domenech N,Willeit P,Shah AM,Jahangiri M,Schaefer L,Fischer JW,Iozzo RV,Viner R,Thum T,Heineke J,Kichler A,Otsu K,Mayr M

Type V Collagen in Health, Disease, and Fibrosis.
Anatomical record (Hoboken, N.J. : 2007)299(5)613-29(2016 May)
Mak KM,Png CY,Lee DJ

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COL5A3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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