Pram1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pram1
Entrez Gene ID	378460
Full Name	PML-RAR alpha-regulated adaptor molecule 1
Synonyms	AY665714
General protein information	Preferred Names PML-RAR alpha-regulated adaptor molecule 1 Names PML-RARA-regulated adapter molecule 1 PRAM-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001002842.2	NP_001002842.2	PML-RARA-regulated adapter molecule 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pram1	NP_001002842.2
Homo sapiens (human)	PRAM1	NP_115528.4
Bos taurus (cattle)	PRAM1	XP_002688937.1
Rattus norvegicus (Norway rat)	Pram1	XP_006241193.1
Pan troglodytes (chimpanzee)	PRAM1	XP_512343.3
	LOC712017	XP_002801105.1
Canis lupus familiaris (dog)	PRAM1	XP_005633283.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Identifying Yersinia YopH-targeted signal transduction pathways that impair neutrophil responses during in vivo murine infection.
Rol?n HG, Durand EA, Mecsas J
Cell host & microbe14(3)306-17(2013 Sep)

PML-RAR? enhances constitutive autophagic activity through inhibiting the Akt/mTOR pathway.
Huang Y, Hou JK, Chen TT, Zhao XY, Yan ZW, Zhang J, Yang J, Kogan SC, Chen GQ
Autophagy7(10)1132-44(2011 Oct)

Oncogenic K-ras cooperates with PML-RAR alpha to induce an acute promyelocytic leukemia-like disease.
Chan IT, Kutok JL, Williams IR, Cohen S, Moore S, Shigematsu H, Ley TJ, Akashi K, Le Beau MM, Gilliland DG
Blood108(5)1708-15(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A yopH mutant survived better in the absence of neutrophils, indicating that neutrophil inactivation by YopH by targeting PRAM-1/SKAP-HOM and SLP-76/Vav/PLCgamma2 signaling hubs may be critical for Yersinia survival.
Title: Identifying Yersinia YopH-targeted signal transduction pathways that impair neutrophil responses during in vivo murine infection.

autophagy contributes to the anti-apoptotic function of the PML-RARalpha protein through inhibiting the Akt/mTOR pathway
Title: PML-RAR? enhances constitutive autophagic activity through inhibiting the Akt/mTOR pathway.

Transgenic mice expressing PML-RAR alpha develop acute promyelocytic leukemia with long latency, low penetrance, and acquired cytogenetic abnormalities.
Title: Oncogenic K-ras cooperates with PML-RAR alpha to induce an acute promyelocytic leukemia-like disease.

The following Pram1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pram1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16696	NM_001002842.2 Latest version!	Mus musculus PML-RAR alpha-regulated adaptor molecule 1 (Pram1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$398.30 $569.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pram1 ( NM_001002842.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001002842.2 Mus musculus PML-RAR alpha-regulated adaptor molecule 1 (Pram1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu16696
Clone ID Related Accession (Same CDS sequence)	NM_001002842.2
Accession Version	NM_001002842.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	PML-RARA-regulated adapter molecule 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY195251.1, AY665714.1 and BE987906.1. On Aug 5, 2009 this sequence version replaced NM_001002842.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. ##Evidence-Data-START## Transcript exon combination :: AY665714.1, BC141100.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAGCA ATCAGGACTT CCGAAACCTC CAAGCTAAGT TCCAAACCTC TCAGCCAGAA 
CTTGGAGAAC TGTTCAGAAA AACCCCAAAG CCTGAGTTAA ACAAAGTCCT GAAGAAATTT 
CCACAGACTG AGTTAAGTGA GCAGCCCAAG AAGTCCTCAC AGTCTGAGCT CAGTGCAGTG 
TCTTTGAAAC CACTGCAGCT GCAGTTTGCA GACCTCCCCA GGAAGCCCCC ACAGCCTGGA 
GTTCTTAAAA AGTCACCACA GCCTGAGTTC CCTCATCTAG CCAACAAGCC TGTACAGGCT 
GAATTCCCAA GGAAGCCTCT ACATCCAGAG TTCACAGGTC TCAAAAAGCC TTCCCAGGCT 
GAGTTCACAG ATCTCAAGAA GCCCCCACAG CCCCAGTTTG CCAGCCTCCC CAAGAAGCCC 
CCAAAACCTG AGTTTGGAGA GCTCTCCAAG AGACCTCCAC AGCTTGAGAC ACCCCAGGAG 
CCTAGTGCTC CTCCTGCCCA GAAGCTCCTG AAGCCCGAGC CTAACAATCC TGCCAGGCCT 
CTAGGAGAGC TCAAACCCAA GATGTTTTGG CACCTTGAGG CCAATGAGGC ACCTAAGAGG 
CCCTTGCCAT CTGAATCCAG TACTTTTCCC AAGAAGCCAC TGCAGCCTGA GGCTGTTGTT 
GGTTTCTCTA GGAAGTCCCA GCCACAGTCA GAGTCCATCG AAGTTTCTCA GACATCCCCC 
TCTAAGTGTG GGTCCAGGGA GCTTGACTCT CACTCCCCAC AGCCTGACAT CAGTACCTTT 
CCTAAGAACA ATGAGAACTT TAGGAAACCT TCATATCCTC AAGCCACTGG TTGCCCCAAG 
TCCCCAAAGC AGCCCATGTT CTATGAGTTC CCCCAGACAC CCCCTCGGAA GCCTGAGTCA 
TGTAACCCCC AGTCTCACTC CCCGCTGCCA GACTTCAATG CATTCCCTAA GAAGCATCCA 
CAGCTCCAGC CAAGTGACCT TACCAGGGCA TCCTCGGAGC CTGAAGTCTG CAAGGTTCCC 
AAAAAGACTC AGAAGCCAGA CCCCAATGTG CTTTCTCAGA AGCCCTCACA GCCAGAATTG 
GGTCACCTCC CTAGAACATC CTCGGACCCC GAGTTCAACT CGCTCCCGAG GAAGTTTCTG 
CAGCCTCAAC ACGGCAAGTT CTTCCAGCCT GAGTTCCCCA AGGGTCTGCC TAGAAAGCCC 
AAGCTTCCTG GTTCAGTGTC TGAATGCTCC CTGCCCTCTG CTAGTGCGGG CTCCAGCCCC 
CAGTGCCCTC TCAGCCCTGG GCTTATAGTC CCTGGGATAC CCCGCTGGAG ATCAGAAGAC 
TTCCAAGTCC AGCGCCCACC CCGACGGCGG CCCCTGCCCT CAGCCAGCAG CCTGGGACAC 
CCCCCAGCCA AGCCCGCACT ACCACCTGGC CCCATCAATA TCCAAAGCTT CAGGAGAGCC 
GCAGCAACAG CCGCAGCGGT ACTGAAGACA GGCTCTTCTA CTGGGACCCA CTTCCAAGCT 
CAACAACCTC AACATATTGC ACAGAACCCG GATGAGATCT ACGAACTGTA TGATGCTGTG 
GAGGCCACAG ATGACTCTAG TATCAGCCCC AGAGGCAGAG ATGAAATGCT GTCTACCCAG 
CAAGCCACCA GATGGCCTCA ACAGGAGCCA GAGCTCAGGA AGAAGGCCAC TCAGCCACAG 
CAGCTGCCAG CTACAGACCC CAAGCTGCTG AAACAGATCA GGAAGGCAGA AAAGGCTGAG 
AGAGAGTTTA GAAAGAAGTT TAAGTTTGAA GGTGAGATTG TGATTCACAC GAAGATGATG 
ATTGACCCCA ATGCCAAGAC CCGGCGTGGG GGTGGCAAGC ACCTGGGCAT CCGGCGTGGA 
GAGATCCTAG AGGTGATCGA GTTCACTAGC AAGGATGAGA TGCTCTGCCG GGACCCCAAG 
GGCAAGTATG GCTATGTGCC CAGAACTGCA CTGTTGCCCT TGGAAACAGA GGTATATGAT 
GACGTCAGCT TTGGGGACCC TCTGGACATG CAACCATTTC CTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002842.2
CDS	120..2147
Translation	MGSNQDFRNLQAKFQTSQPELGELFRKTPKPELNKVLKKFPQTELSEQPKKSSQSELSAVSLKPLQLQFADLPRKPPQPGVLKKSPQPEFPHLANKPVQAEFPRKPLHPEFTGLKKPSQAEFTDLKKPPQPQFASLPKKPPKPEFGELSKRPPQLETPQEPSAPPAQKLLKPEPNNPARPLGELKPKMFWHLEANEAPKRPLPSESSTFPKKPLQPEAVVGFSRKSQPQSESIEVSQTSPSKCGSRELDSHSPQPDISTFPKNNENFRKPSYPQATGCPKSPKQPMFYEFPQTPPRKPESCNPQSHSPLPDFNAFPKKHPQLQPSDLTRASSEPEVCKVPKKTQKPDPNVLSQKPSQPELGHLPRTSSDPEFNSLPRKFLQPQHGKFFQPEFPKGLPRKPKLPGSVSECSLPSASAGSSPQCPLSPGLIVPGIPRWRSEDFQVQRPPRRRPLPSASSLGHPPAKPALPPGPINIQSFRRAAATAAAVLKTGSSTGTHFQAQQPQHIAQNPDEIYELYDAVEATDDSSISPRGRDEMLSTQQATRWPQQEPELRKKATQPQQLPATDPKLLKQIRKAEKAEREFRKKFKFEGEIVIHTKMMIDPNAKTRRGGGKHLGIRRGEILEVIEFTSKDEMLCRDPKGKYGYVPRTALLPLETEVYDDVSFGDPLDMQPFPR

Target ORF information:

RefSeq Version	NM_001002842.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus PML-RAR alpha-regulated adaptor molecule 1 (Pram1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002842.2

ORF Insert Sequence:

ATGGGGAGCA ATCAGGACTT CCGAAACCTC CAAGCTAAGT TCCAAACCTC TCAGCCAGAA 
CTTGGAGAAC TGTTCAGAAA AACCCCAAAG CCTGAGTTAA ACAAAGTCCT GAAGAAATTT 
CCACAGACTG AGTTAAGTGA GCAGCCCAAG AAGTCCTCAC AGTCTGAGCT CAGTGCAGTG 
TCTTTGAAAC CACTGCAGCT GCAGTTTGCA GACCTCCCCA GGAAGCCCCC ACAGCCTGGA 
GTTCTTAAAA AGTCACCACA GCCTGAGTTC CCTCATCTAG CCAACAAGCC TGTACAGGCT 
GAATTCCCAA GGAAGCCTCT ACATCCAGAG TTCACAGGTC TCAAAAAGCC TTCCCAGGCT 
GAGTTCACAG ATCTCAAGAA GCCCCCACAG CCCCAGTTTG CCAGCCTCCC CAAGAAGCCC 
CCAAAACCTG AGTTTGGAGA GCTCTCCAAG AGACCTCCAC AGCTTGAGAC ACCCCAGGAG 
CCTAGTGCTC CTCCTGCCCA GAAGCTCCTG AAGCCCGAGC CTAACAATCC TGCCAGGCCT 
CTAGGAGAGC TCAAACCCAA GATGTTTTGG CACCTTGAGG CCAATGAGGC ACCTAAGAGG 
CCCTTGCCAT CTGAATCCAG TACTTTTCCC AAGAAGCCAC TGCAGCCTGA GGCTGTTGTT 
GGTTTCTCTA GGAAGTCCCA GCCACAGTCA GAGTCCATCG AAGTTTCTCA GACATCCCCC 
TCTAAGTGTG GGTCCAGGGA GCTTGACTCT CACTCCCCAC AGCCTGACAT CAGTACCTTT 
CCTAAGAACA ATGAGAACTT TAGGAAACCT TCATATCCTC AAGCCACTGG TTGCCCCAAG 
TCCCCAAAGC AGCCCATGTT CTATGAGTTC CCCCAGACAC CCCCTCGGAA GCCTGAGTCA 
TGTAACCCCC AGTCTCACTC CCCGCTGCCA GACTTCAATG CATTCCCTAA GAAGCATCCA 
CAGCTCCAGC CAAGTGACCT TACCAGGGCA TCCTCGGAGC CTGAAGTCTG CAAGGTTCCC 
AAAAAGACTC AGAAGCCAGA CCCCAATGTG CTTTCTCAGA AGCCCTCACA GCCAGAATTG 
GGTCACCTCC CTAGAACATC CTCGGACCCC GAGTTCAACT CGCTCCCGAG GAAGTTTCTG 
CAGCCTCAAC ACGGCAAGTT CTTCCAGCCT GAGTTCCCCA AGGGTCTGCC TAGAAAGCCC 
AAGCTTCCTG GTTCAGTGTC TGAATGCTCC CTGCCCTCTG CTAGTGCGGG CTCCAGCCCC 
CAGTGCCCTC TCAGCCCTGG GCTTATAGTC CCTGGGATAC CCCGCTGGAG ATCAGAAGAC 
TTCCAAGTCC AGCGCCCACC CCGACGGCGG CCCCTGCCCT CAGCCAGCAG CCTGGGACAC 
CCCCCAGCCA AGCCCGCACT ACCACCTGGC CCCATCAATA TCCAAAGCTT CAGGAGAGCC 
GCAGCAACAG CCGCAGCGGT ACTGAAGACA GGCTCTTCTA CTGGGACCCA CTTCCAAGCT 
CAACAACCTC AACATATTGC ACAGAACCCG GATGAGATCT ACGAACTGTA TGATGCTGTG 
GAGGCCACAG ATGACTCTAG TATCAGCCCC AGAGGCAGAG ATGAAATGCT GTCTACCCAG 
CAAGCCACCA GATGGCCTCA ACAGGAGCCA GAGCTCAGGA AGAAGGCCAC TCAGCCACAG 
CAGCTGCCAG CTACAGACCC CAAGCTGCTG AAACAGATCA GGAAGGCAGA AAAGGCTGAG 
AGAGAGTTTA GAAAGAAGTT TAAGTTTGAA GGTGAGATTG TGATTCACAC GAAGATGATG 
ATTGACCCCA ATGCCAAGAC CCGGCGTGGG GGTGGCAAGC ACCTGGGCAT CCGGCGTGGA 
GAGATCCTAG AGGTGATCGA GTTCACTAGC AAGGATGAGA TGCTCTGCCG GGACCCCAAG 
GGCAAGTATG GCTATGTGCC CAGAACTGCA CTGTTGCCCT TGGAAACAGA GGTATATGAT 
GACGTCAGCT TTGGGGACCC TCTGGACATG CAACCATTTC CTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Identifying Yersinia YopH-targeted signal transduction pathways that impair neutrophil responses during in vivo murine infection.
Cell host & microbe14(3)306-17(2013 Sep)
Rol?n HG,Durand EA,Mecsas J

PML-RAR? enhances constitutive autophagic activity through inhibiting the Akt/mTOR pathway.
Autophagy7(10)1132-44(2011 Oct)
Huang Y,Hou JK,Chen TT,Zhao XY,Yan ZW,Zhang J,Yang J,Kogan SC,Chen GQ

Oncogenic K-ras cooperates with PML-RAR alpha to induce an acute promyelocytic leukemia-like disease.
Blood108(5)1708-15(2006 Sep)
Chan IT,Kutok JL,Williams IR,Cohen S,Moore S,Shigematsu H,Ley TJ,Akashi K,Le Beau MM,Gilliland DG