PRAM1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRAM1
Entrez Gene ID	84106
Full Name	PML-RARA regulated adaptor molecule 1
Synonyms	PML-RAR,PRAM-1
General protein information	Preferred Names PML-RARA regulated adaptor molecule 1 Names PML-RARA-regulated adapter molecule 1 PML-RARA target gene encoding an Adaptor Molecule-1 PRAM
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.2
Summary	The protein encoded by this gene is similar to FYN binding protein (FYB/SLAP-130), an adaptor protein involved in T cell receptor mediated signaling. This gene is expressed and regulated during normal myelopoiesis. The expression of this gene is induced by retinoic acid and is inhibited by the expression of PML-RARalpha, a fusion protein of promyelocytic leukemia (PML) and the retinoic acid receptor-alpha (RARalpha). [provided by RefSeq, Jul 2008].
Disorder MIM:	606466

mRNA and Protein(s)

mRNA	Protein	Name
XM_011528352.2	XP_011526654.1	PML-RARA-regulated adapter molecule 1 isoform X1
XM_005272502.2	XP_005272559.1	PML-RARA-regulated adapter molecule 1 isoform X2
NM_032152.5	NP_115528.4	PML-RARA-regulated adapter molecule 1
NM_032152.4	NP_115528.4	PML-RARA-regulated adapter molecule 1
XM_011528353.2	XP_011526655.1	PML-RARA-regulated adapter molecule 1 isoform X3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pram1	NP_001002842.2
Homo sapiens (human)	PRAM1	NP_115528.4
Bos taurus (cattle)	PRAM1	XP_002688937.1
Rattus norvegicus (Norway rat)	Pram1	XP_006241193.1
Pan troglodytes (chimpanzee)	PRAM1	XP_512343.3
	LOC712017	XP_002801105.1
Canis lupus familiaris (dog)	PRAM1	XP_005633283.1

Gene Ontology
Bibliography

Related articles in PubMed

Spinal promyelocytic sarcoma as the initial manifestation of acute promyelocytic leukaemia.
Villase?or-Ledezma J, Amosa-Delgado M, Ru?z-Gin?s JA, ?lvarez-Salgado JA
Neurologia (Barcelona, Spain)(2017 Apr)

Regulation of NF-?B by PML and PML-RAR?.
Ahmed A, Wan X, Mitxitorena I, Lindsay AJ, Paolo Pandolfi P, McCaffrey MW, Keeshan K, Chen YH, Carmody RJ
Scientific reports744539(2017 Mar)

Absolute quantification of the pretreatment PML-RARA transcript defines the relapse risk in acute promyelocytic leukemia.
Albano F, Zagaria A, Anelli L, Coccaro N, Tota G, Brunetti C, Minervini CF, Impera L, Minervini A, Cellamare A, Orsini P, Cumbo C, Casieri P, Specchia G
Oncotarget6(15)13269-77(2015 May)

Development and validation of a 3-Plex RT-qPCR assay for the simultaneous detection and quantitation of the three PML-RARa fusion transcripts in acute promyelocytic leukemia.
Chen Z, Tong Y, Li Y, Gao Q, Wang Q, Fu C, Xia Z
PloS one10(3)e0122530(2015)

MIR125B1 represses the degradation of the PML-RARA oncoprotein by an autophagy-lysosomal pathway in acute promyelocytic leukemia.
Zeng CW, Chen ZH, Zhang XJ, Han BW, Lin KY, Li XJ, Wei PP, Zhang H, Li Y, Chen YQ
Autophagy10(10)1726-37(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results show that the pretreatment PML-RARA molecular burden could therefore be used to improve risk stratification in order to develop more individualized treatment regimens for high-risk APL cases.
Title: Absolute quantification of the pretreatment PML-RARA transcript defines the relapse risk in acute promyelocytic leukemia.

The 3-plex RT-qPCR assay is a specific, sensitive, stable, and cost-effective method that can be used for the rapid diagnosis and treatment monitoring of acute promyelocytic leukemia with PML-RARa
Title: Development and validation of a 3-Plex RT-qPCR assay for the simultaneous detection and quantitation of the three PML-RARa fusion transcripts in acute promyelocytic leukemia.

The MIR125B1-mediated blockade of PML-RARA proteolysis was regulated via an autophagy-lysosomal pathway, contributing to the inhibition of acute promyelocytic leukemia differentiation.
Title: MIR125B1 represses the degradation of the PML-RARA oncoprotein by an autophagy-lysosomal pathway in acute promyelocytic leukemia.

autophagy contributes to the anti-apoptotic function of the PML-RARalpha protein through inhibiting the Akt/mTOR pathway
Title: PML-RAR? enhances constitutive autophagic activity through inhibiting the Akt/mTOR pathway.

We conclude that our sp-RQ-PCR is specific enough to identify various forms of PML-RARalpha and yields no false-positive results.
Title: Quantitative detection of PML-RARalpha fusion transcript by real-time PCR with a single primer pair.

The following PRAM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRAM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01903	NM_032152.5 Latest version!	Homo sapiens PML-RARA regulated adaptor molecule 1 (PRAM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$265.30 $379.00
OHu51859	XM_005272502.2 Latest version!	Homo sapiens PML-RARA regulated adaptor molecule 1 (PRAM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu73332	XM_011528352.2 Latest version!	Homo sapiens PML-RARA regulated adaptor molecule 1 (PRAM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu73333	XM_011528353.2 Latest version!	Homo sapiens PML-RARA regulated adaptor molecule 1 (PRAM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
View Old Accession Versions >>
OHu01903	NM_032152.4	Homo sapiens PML-RARA regulated adaptor molecule 1 (PRAM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRAM1 ( NM_032152.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_115528.4 Homo sapiens PML-RARA regulated adaptor molecule 1 (PRAM1), mRNA.

Price & Availability↑

CloneID	OHu01903
Clone ID Related Accession (Same CDS sequence)	NM_032152.5 , NM_032152.4
Accession Version	NM_032152.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	PML-RARA-regulated adapter molecule 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ272324.1, DA350460.1, AC092298.2, AK308227.1 and BC028012.1. On Nov 22, 2018 this sequence version replaced NM_032152.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC028012.1, AK308227.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000423345.5/ ENSP00000408342.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCCATC ACCTGCCTGC AGCCATGGAG AGCCATCAGG ACTTCCGGAG CATCAAAGCA 
AAGTTCCAGG CCTCTCAGCC GGAGCCCAGC GACCTGCCCA AAAAACCTCC GAAGCCTGAG 
TTTGGTAAAC TGAAGAAGTT CTCCCAGCCT GAGCTAAGCG AGCACCCCAA GAAGGCCCCG 
CTGCCTGAGT TTGGTGCAGT GTCCTTGAAG CCCCCGCCGC CTGAGGTCAC TGACCTCCCC 
AAGAAGCCCC CGCCGCCTGA GGTCACTGAC CTCCCCAAGA AGCCCCCGCC GCCTGAGGTC 
ACTGACCTCC CCAAGAAGCC CCCGCCGCCT GAGGTCACTG ACCTCCCCAA GAAGCCGTCC 
AAACTGGAGT TGAGTGACCT CTCCAAGAAG TTCCCACAGC TGGGGGCCAC TCCGTTTCCA 
AGGAAGCCCC TGCAGCCTGA GGTCGGTGAG GCCCCTTTGA AGGCCTCGCT GCCGGAGCCT 
GGTGCGCCGG CCCGGAAACC CCTGCAGCCC GACGAACTCA GTCACCCCGC CAGACCCCCC 
TCCGAACCCA AATCCGGCGC ATTCCCCAGG AAGCTCTGGC AACCCGAGGC CGGTGAGGCT 
ACCCCGAGGT CCCCGCAGCC TGAGTTGAGT ACCTTTCCCA AGAAGCCTGC GCAGCCTGAG 
TTCAACGTGT ACCCCAAAAA GCCTCCGCAG CCTCAGGTCG GTGGCCTCCC TAAGAAGTCC 
GTGCCGCAGC CTGAGTTCAG CGAGGCCGCT CAGACTCCCC TCTGGAAGCC TCAGTCCAGC 
GAGCCGAAGC GCGACTCCAG CGCCTTTCCC AAAAAGGCCT CCCAGCCTCC GCTGAGTGAC 
TTTCCCAAGA AGCCTCCGCA GCCTGAGCTT GGGGACCTCA CCAGGACCTC CTCAGAGCCC 
GAAGTCAGCG TGCTTCCCAA GAGGCCGCGG CCGGCCGAAT TCAAAGCGCT CTCCAAGAAG 
CCCCCGCAGC CCGAGCTGGG CGGCCTCCCC AGGACCTCCT CAGAGCCCGA GTTCAACTCA 
CTCCCCAGGA AGCTGCTGCA GCCGGAGCGC CGGGGGCCAC CCCGCAAGTT CTCACAGCCT 
GAGCCCAGCG CTGTCCTCAA GAGACACCCG CAGCCTGAGT TCTTCGGTGA TCTCCCTCGA 
AAGCCTCCAC TCCCCAGCTC CGCTTCCGAG AGCTCACTGC CTGCGGCCGT GGCCGGCTTC 
AGCTCCCGGC ACCCGCTCAG CCCTGGGTTT GGAGCGGCTG GGACACCCCG CTGGAGGTCA 
GGAGGCCTGG TTCACAGTGG AGGGGCCAGG CCAGGCCTCA GACCCAGCCA TCCACCCCGG 
CGGAGGCCTC TGCCCCCTGC CAGCAGCCTG GGACACCCTC CAGCCAAGCC CCCGCTGCCC 
CCGGGGCCCG TGGATATGCA GAGCTTTCGG AGACCCTCTG CAGCATCCAT AGATCTACGG 
AGGACCCGCT CGGCCGCTGG GCTCCACTTC CAGGACCGAC AGCCTGAAGA CATCCCGCAG 
GTCCCAGATG AGATCTACGA GCTGTATGAC GATGTGGAAC CCAGAGATGA CTCCAGCCCC 
AGCCCCAAGG GCAGAGATGA AGCGCCCTCA GTTCAGCAAG CCGCCAGGAG GCCACCACAA 
GACCCAGCGC TCAGGAAGGA GAAGGATCCC CAGCCACAGC AGTTGCCACC CATGGACCCA 
AAGTTGCTGA AGCAGCTGAG GAAGGCAGAG AAGGCCGAGA GGGAGTTCCG GAAGAAGTTC 
AAGTTTGAAG GGGAGATCGT GGTTCACACG AAGATGATGA TCGACCCCAA CGCTAAGACA 
CGTCGCGGGG GTGGCAAGCA CCTCGGGATC CGGCGCGGGG AGATCCTGGA GGTGATCGAG 
TTCACCAGCA ATGAGGAGAT GCTGTGCCGG GACCCCAAAG GCAAATATGG CTACGTGCCC 
AGAACAGCGC TCCTGCCCCT GGAGACGGAG GTGTACGATG ATGTCGACTT CTGCGATCCC 
CTGGAAAACC AACCACTCCC CCTGGGACGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115528.4
CDS	50..2062
Translation	MAHHLPAAMESHQDFRSIKAKFQASQPEPSDLPKKPPKPEFGKLKKFSQPELSEHPKKAPLPEFGAVSLKPPPPEVTDLPKKPPPPEVTDLPKKPPPPEVTDLPKKPPPPEVTDLPKKPSKLELSDLSKKFPQLGATPFPRKPLQPEVGEAPLKASLPEPGAPARKPLQPDELSHPARPPSEPKSGAFPRKLWQPEAGEATPRSPQPELSTFPKKPAQPEFNVYPKKPPQPQVGGLPKKSVPQPEFSEAAQTPLWKPQSSEPKRDSSAFPKKASQPPLSDFPKKPPQPELGDLTRTSSEPEVSVLPKRPRPAEFKALSKKPPQPELGGLPRTSSEPEFNSLPRKLLQPERRGPPRKFSQPEPSAVLKRHPQPEFFGDLPRKPPLPSSASESSLPAAVAGFSSRHPLSPGFGAAGTPRWRSGGLVHSGGARPGLRPSHPPRRRPLPPASSLGHPPAKPPLPPGPVDMQSFRRPSAASIDLRRTRSAAGLHFQDRQPEDIPQVPDEIYELYDDVEPRDDSSPSPKGRDEAPSVQQAARRPPQDPALRKEKDPQPQQLPPMDPKLLKQLRKAEKAEREFRKKFKFEGEIVVHTKMMIDPNAKTRRGGGKHLGIRRGEILEVIEFTSNEEMLCRDPKGKYGYVPRTALLPLETEVYDDVDFCDPLENQPLPLGR

Target ORF information:

RefSeq Version	NM_032152.5
Organism	Homo sapiens(human)
Definition	Homo sapiens PML-RARA regulated adaptor molecule 1 (PRAM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032152.5

ORF Insert Sequence:

ATGGCCCATC ACCTGCCTGC AGCCATGGAG AGCCATCAGG ACTTCCGGAG CATCAAAGCA 
AAGTTCCAGG CCTCTCAGCC GGAGCCCAGC GACCTGCCCA AAAAACCTCC GAAGCCTGAG 
TTTGGTAAAC TGAAGAAGTT CTCCCAGCCT GAGCTAAGCG AGCACCCCAA GAAGGCCCCG 
CTGCCTGAGT TTGGTGCAGT GTCCTTGAAG CCCCCGCCGC CTGAGGTCAC TGACCTCCCC 
AAGAAGCCCC CGCCGCCTGA GGTCACTGAC CTCCCCAAGA AGCCCCCGCC GCCTGAGGTC 
ACTGACCTCC CCAAGAAGCC CCCGCCGCCT GAGGTCACTG ACCTCCCCAA GAAGCCGTCC 
AAACTGGAGT TGAGTGACCT CTCCAAGAAG TTCCCACAGC TGGGGGCCAC TCCGTTTCCA 
AGGAAGCCCC TGCAGCCTGA GGTCGGTGAG GCCCCTTTGA AGGCCTCGCT GCCGGAGCCT 
GGTGCGCCGG CCCGGAAACC CCTGCAGCCC GACGAACTCA GTCACCCCGC CAGACCCCCC 
TCCGAACCCA AATCCGGCGC ATTCCCCAGG AAGCTCTGGC AACCCGAGGC CGGTGAGGCT 
ACCCCGAGGT CCCCGCAGCC TGAGTTGAGT ACCTTTCCCA AGAAGCCTGC GCAGCCTGAG 
TTCAACGTGT ACCCCAAAAA GCCTCCGCAG CCTCAGGTCG GTGGCCTCCC TAAGAAGTCC 
GTGCCGCAGC CTGAGTTCAG CGAGGCCGCT CAGACTCCCC TCTGGAAGCC TCAGTCCAGC 
GAGCCGAAGC GCGACTCCAG CGCCTTTCCC AAAAAGGCCT CCCAGCCTCC GCTGAGTGAC 
TTTCCCAAGA AGCCTCCGCA GCCTGAGCTT GGGGACCTCA CCAGGACCTC CTCAGAGCCC 
GAAGTCAGCG TGCTTCCCAA GAGGCCGCGG CCGGCCGAAT TCAAAGCGCT CTCCAAGAAG 
CCCCCGCAGC CCGAGCTGGG CGGCCTCCCC AGGACCTCCT CAGAGCCCGA GTTCAACTCA 
CTCCCCAGGA AGCTGCTGCA GCCGGAGCGC CGGGGGCCAC CCCGCAAGTT CTCACAGCCT 
GAGCCCAGCG CTGTCCTCAA GAGACACCCG CAGCCTGAGT TCTTCGGTGA TCTCCCTCGA 
AAGCCTCCAC TCCCCAGCTC CGCTTCCGAG AGCTCACTGC CTGCGGCCGT GGCCGGCTTC 
AGCTCCCGGC ACCCGCTCAG CCCTGGGTTT GGAGCGGCTG GGACACCCCG CTGGAGGTCA 
GGAGGCCTGG TTCACAGTGG AGGGGCCAGG CCAGGCCTCA GACCCAGCCA TCCACCCCGG 
CGGAGGCCTC TGCCCCCTGC CAGCAGCCTG GGACACCCTC CAGCCAAGCC CCCGCTGCCC 
CCGGGGCCCG TGGATATGCA GAGCTTTCGG AGACCCTCTG CAGCATCCAT AGATCTACGG 
AGGACCCGCT CGGCCGCTGG GCTCCACTTC CAGGACCGAC AGCCTGAAGA CATCCCGCAG 
GTCCCAGATG AGATCTACGA GCTGTATGAC GATGTGGAAC CCAGAGATGA CTCCAGCCCC 
AGCCCCAAGG GCAGAGATGA AGCGCCCTCA GTTCAGCAAG CCGCCAGGAG GCCACCACAA 
GACCCAGCGC TCAGGAAGGA GAAGGATCCC CAGCCACAGC AGTTGCCACC CATGGACCCA 
AAGTTGCTGA AGCAGCTGAG GAAGGCAGAG AAGGCCGAGA GGGAGTTCCG GAAGAAGTTC 
AAGTTTGAAG GGGAGATCGT GGTTCACACG AAGATGATGA TCGACCCCAA CGCTAAGACA 
CGTCGCGGGG GTGGCAAGCA CCTCGGGATC CGGCGCGGGG AGATCCTGGA GGTGATCGAG 
TTCACCAGCA ATGAGGAGAT GCTGTGCCGG GACCCCAAAG GCAAATATGG CTACGTGCCC 
AGAACAGCGC TCCTGCCCCT GGAGACGGAG GTGTACGATG ATGTCGACTT CTGCGATCCC 
CTGGAAAACC AACCACTCCC CCTGGGACGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRAM1 ( NM_032152.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_115528.4 Homo sapiens PML-RARA regulated adaptor molecule 1 (PRAM1), mRNA.

Price & Availability↑

CloneID	OHu01903
Clone ID Related Accession (Same CDS sequence)	NM_032152.5 , NM_032152.4
Accession Version	NM_032152.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	PML-RARA-regulated adapter molecule 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ272324.1, DA350460.1, AC092298.2, AK308227.1 and BC028012.1. On Aug 6, 2010 this sequence version replaced NM_032152.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC028012.1, AK308227.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCATC ACCTGCCTGC AGCCATGGAG AGCCATCAGG ACTTCCGGAG CATCAAAGCA 
AAGTTCCAGG CCTCTCAGCC GGAGCCCAGC GACCTGCCCA AAAAACCTCC GAAGCCTGAG 
TTTGGTAAAC TGAAGAAGTT CTCCCAGCCT GAGCTAAGCG AGCACCCCAA GAAGGCCCCG 
CTGCCTGAGT TTGGTGCAGT GTCCTTGAAG CCCCCGCCGC CTGAGGTCAC TGACCTCCCC 
AAGAAGCCCC CGCCGCCTGA GGTCACTGAC CTCCCCAAGA AGCCCCCGCC GCCTGAGGTC 
ACTGACCTCC CCAAGAAGCC CCCGCCGCCT GAGGTCACTG ACCTCCCCAA GAAGCCGTCC 
AAACTGGAGT TGAGTGACCT CTCCAAGAAG TTCCCACAGC TGGGGGCCAC TCCGTTTCCA 
AGGAAGCCCC TGCAGCCTGA GGTCGGTGAG GCCCCTTTGA AGGCCTCGCT GCCGGAGCCT 
GGTGCGCCGG CCCGGAAACC CCTGCAGCCC GACGAACTCA GTCACCCCGC CAGACCCCCC 
TCCGAACCCA AATCCGGCGC ATTCCCCAGG AAGCTCTGGC AACCCGAGGC CGGTGAGGCT 
ACCCCGAGGT CCCCGCAGCC TGAGTTGAGT ACCTTTCCCA AGAAGCCTGC GCAGCCTGAG 
TTCAACGTGT ACCCCAAAAA GCCTCCGCAG CCTCAGGTCG GTGGCCTCCC TAAGAAGTCC 
GTGCCGCAGC CTGAGTTCAG CGAGGCCGCT CAGACTCCCC TCTGGAAGCC TCAGTCCAGC 
GAGCCGAAGC GCGACTCCAG CGCCTTTCCC AAAAAGGCCT CCCAGCCTCC GCTGAGTGAC 
TTTCCCAAGA AGCCTCCGCA GCCTGAGCTT GGGGACCTCA CCAGGACCTC CTCAGAGCCC 
GAAGTCAGCG TGCTTCCCAA GAGGCCGCGG CCGGCCGAAT TCAAAGCGCT CTCCAAGAAG 
CCCCCGCAGC CCGAGCTGGG CGGCCTCCCC AGGACCTCCT CAGAGCCCGA GTTCAACTCA 
CTCCCCAGGA AGCTGCTGCA GCCGGAGCGC CGGGGGCCAC CCCGCAAGTT CTCACAGCCT 
GAGCCCAGCG CTGTCCTCAA GAGACACCCG CAGCCTGAGT TCTTCGGTGA TCTCCCTCGA 
AAGCCTCCAC TCCCCAGCTC CGCTTCCGAG AGCTCACTGC CTGCGGCCGT GGCCGGCTTC 
AGCTCCCGGC ACCCGCTCAG CCCTGGGTTT GGAGCGGCTG GGACACCCCG CTGGAGGTCA 
GGAGGCCTGG TTCACAGTGG AGGGGCCAGG CCAGGCCTCA GACCCAGCCA TCCACCCCGG 
CGGAGGCCTC TGCCCCCTGC CAGCAGCCTG GGACACCCTC CAGCCAAGCC CCCGCTGCCC 
CCGGGGCCCG TGGATATGCA GAGCTTTCGG AGACCCTCTG CAGCATCCAT AGATCTACGG 
AGGACCCGCT CGGCCGCTGG GCTCCACTTC CAGGACCGAC AGCCTGAAGA CATCCCGCAG 
GTCCCAGATG AGATCTACGA GCTGTATGAC GATGTGGAAC CCAGAGATGA CTCCAGCCCC 
AGCCCCAAGG GCAGAGATGA AGCGCCCTCA GTTCAGCAAG CCGCCAGGAG GCCACCACAA 
GACCCAGCGC TCAGGAAGGA GAAGGATCCC CAGCCACAGC AGTTGCCACC CATGGACCCA 
AAGTTGCTGA AGCAGCTGAG GAAGGCAGAG AAGGCCGAGA GGGAGTTCCG GAAGAAGTTC 
AAGTTTGAAG GGGAGATCGT GGTTCACACG AAGATGATGA TCGACCCCAA CGCTAAGACA 
CGTCGCGGGG GTGGCAAGCA CCTCGGGATC CGGCGCGGGG AGATCCTGGA GGTGATCGAG 
TTCACCAGCA ATGAGGAGAT GCTGTGCCGG GACCCCAAAG GCAAATATGG CTACGTGCCC 
AGAACAGCGC TCCTGCCCCT GGAGACGGAG GTGTACGATG ATGTCGACTT CTGCGATCCC 
CTGGAAAACC AACCACTCCC CCTGGGACGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115528.4
CDS	64..2076
Translation	MAHHLPAAMESHQDFRSIKAKFQASQPEPSDLPKKPPKPEFGKLKKFSQPELSEHPKKAPLPEFGAVSLKPPPPEVTDLPKKPPPPEVTDLPKKPPPPEVTDLPKKPPPPEVTDLPKKPSKLELSDLSKKFPQLGATPFPRKPLQPEVGEAPLKASLPEPGAPARKPLQPDELSHPARPPSEPKSGAFPRKLWQPEAGEATPRSPQPELSTFPKKPAQPEFNVYPKKPPQPQVGGLPKKSVPQPEFSEAAQTPLWKPQSSEPKRDSSAFPKKASQPPLSDFPKKPPQPELGDLTRTSSEPEVSVLPKRPRPAEFKALSKKPPQPELGGLPRTSSEPEFNSLPRKLLQPERRGPPRKFSQPEPSAVLKRHPQPEFFGDLPRKPPLPSSASESSLPAAVAGFSSRHPLSPGFGAAGTPRWRSGGLVHSGGARPGLRPSHPPRRRPLPPASSLGHPPAKPPLPPGPVDMQSFRRPSAASIDLRRTRSAAGLHFQDRQPEDIPQVPDEIYELYDDVEPRDDSSPSPKGRDEAPSVQQAARRPPQDPALRKEKDPQPQQLPPMDPKLLKQLRKAEKAEREFRKKFKFEGEIVVHTKMMIDPNAKTRRGGGKHLGIRRGEILEVIEFTSNEEMLCRDPKGKYGYVPRTALLPLETEVYDDVDFCDPLENQPLPLGR

Target ORF information:

RefSeq Version	NM_032152.4
Organism	Homo sapiens(human)
Definition	Homo sapiens PML-RARA regulated adaptor molecule 1 (PRAM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032152.4

ORF Insert Sequence:

ATGGCCCATC ACCTGCCTGC AGCCATGGAG AGCCATCAGG ACTTCCGGAG CATCAAAGCA 
AAGTTCCAGG CCTCTCAGCC GGAGCCCAGC GACCTGCCCA AAAAACCTCC GAAGCCTGAG 
TTTGGTAAAC TGAAGAAGTT CTCCCAGCCT GAGCTAAGCG AGCACCCCAA GAAGGCCCCG 
CTGCCTGAGT TTGGTGCAGT GTCCTTGAAG CCCCCGCCGC CTGAGGTCAC TGACCTCCCC 
AAGAAGCCCC CGCCGCCTGA GGTCACTGAC CTCCCCAAGA AGCCCCCGCC GCCTGAGGTC 
ACTGACCTCC CCAAGAAGCC CCCGCCGCCT GAGGTCACTG ACCTCCCCAA GAAGCCGTCC 
AAACTGGAGT TGAGTGACCT CTCCAAGAAG TTCCCACAGC TGGGGGCCAC TCCGTTTCCA 
AGGAAGCCCC TGCAGCCTGA GGTCGGTGAG GCCCCTTTGA AGGCCTCGCT GCCGGAGCCT 
GGTGCGCCGG CCCGGAAACC CCTGCAGCCC GACGAACTCA GTCACCCCGC CAGACCCCCC 
TCCGAACCCA AATCCGGCGC ATTCCCCAGG AAGCTCTGGC AACCCGAGGC CGGTGAGGCT 
ACCCCGAGGT CCCCGCAGCC TGAGTTGAGT ACCTTTCCCA AGAAGCCTGC GCAGCCTGAG 
TTCAACGTGT ACCCCAAAAA GCCTCCGCAG CCTCAGGTCG GTGGCCTCCC TAAGAAGTCC 
GTGCCGCAGC CTGAGTTCAG CGAGGCCGCT CAGACTCCCC TCTGGAAGCC TCAGTCCAGC 
GAGCCGAAGC GCGACTCCAG CGCCTTTCCC AAAAAGGCCT CCCAGCCTCC GCTGAGTGAC 
TTTCCCAAGA AGCCTCCGCA GCCTGAGCTT GGGGACCTCA CCAGGACCTC CTCAGAGCCC 
GAAGTCAGCG TGCTTCCCAA GAGGCCGCGG CCGGCCGAAT TCAAAGCGCT CTCCAAGAAG 
CCCCCGCAGC CCGAGCTGGG CGGCCTCCCC AGGACCTCCT CAGAGCCCGA GTTCAACTCA 
CTCCCCAGGA AGCTGCTGCA GCCGGAGCGC CGGGGGCCAC CCCGCAAGTT CTCACAGCCT 
GAGCCCAGCG CTGTCCTCAA GAGACACCCG CAGCCTGAGT TCTTCGGTGA TCTCCCTCGA 
AAGCCTCCAC TCCCCAGCTC CGCTTCCGAG AGCTCACTGC CTGCGGCCGT GGCCGGCTTC 
AGCTCCCGGC ACCCGCTCAG CCCTGGGTTT GGAGCGGCTG GGACACCCCG CTGGAGGTCA 
GGAGGCCTGG TTCACAGTGG AGGGGCCAGG CCAGGCCTCA GACCCAGCCA TCCACCCCGG 
CGGAGGCCTC TGCCCCCTGC CAGCAGCCTG GGACACCCTC CAGCCAAGCC CCCGCTGCCC 
CCGGGGCCCG TGGATATGCA GAGCTTTCGG AGACCCTCTG CAGCATCCAT AGATCTACGG 
AGGACCCGCT CGGCCGCTGG GCTCCACTTC CAGGACCGAC AGCCTGAAGA CATCCCGCAG 
GTCCCAGATG AGATCTACGA GCTGTATGAC GATGTGGAAC CCAGAGATGA CTCCAGCCCC 
AGCCCCAAGG GCAGAGATGA AGCGCCCTCA GTTCAGCAAG CCGCCAGGAG GCCACCACAA 
GACCCAGCGC TCAGGAAGGA GAAGGATCCC CAGCCACAGC AGTTGCCACC CATGGACCCA 
AAGTTGCTGA AGCAGCTGAG GAAGGCAGAG AAGGCCGAGA GGGAGTTCCG GAAGAAGTTC 
AAGTTTGAAG GGGAGATCGT GGTTCACACG AAGATGATGA TCGACCCCAA CGCTAAGACA 
CGTCGCGGGG GTGGCAAGCA CCTCGGGATC CGGCGCGGGG AGATCCTGGA GGTGATCGAG 
TTCACCAGCA ATGAGGAGAT GCTGTGCCGG GACCCCAAAG GCAAATATGG CTACGTGCCC 
AGAACAGCGC TCCTGCCCCT GGAGACGGAG GTGTACGATG ATGTCGACTT CTGCGATCCC 
CTGGAAAACC AACCACTCCC CCTGGGACGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRAM1 ( XM_005272502.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005272559.1 Homo sapiens PML-RARA regulated adaptor molecule 1 (PRAM1), transcript variant X2, mRNA.

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CloneID	OHu51859
Clone ID Related Accession (Same CDS sequence)	XM_005272502.2
Accession Version	XM_005272502.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	PML-RARA-regulated adapter molecule 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005272502.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCATC ACCTGCCTGC AGCCATGGAG AGCCATCAGG ACTTCCGGAG CATCAAAGCA 
AAGTTCCAGG CCTCTCAGCC GGAGCCCAGC GACCTGCCCA AAAAACCTCC GAAGCCTGAG 
TTTGGTAAAC TGAAGAAGTT CTCCCAGCCT GAGCTAAGCG AGCACCCCAA GAAGGCCCCG 
CTGCCTGAGT TTGGTGCAGT GTCCTTGAAG CCCCCGCCGC CTGAGGTCAC TGACCTCCCC 
AAGAAGCCCC CGCCGCCTGA GGTCACTGAC CTCCCCAAGA AGCCCCCGCC GCCTGAGGTC 
ACTGACCTCC CCAAGAAGCC CCCGCCGCCT GAGGTCACTG ACCTCCCCAA GAAGCCGTCC 
AAACTGGAGT TGAGTGACCT CTCCAAGAAG TTCCCACAGC TGGGGGCCAC TCCGTTTCCA 
AGGAAGCCCC TGCAGCCTGA GGTCGGTGAG GCCCCTTTGA AGGCCTCGCT GCCGGAGCCT 
GGTGCGCCGG CCCGGAAACC CCTGCAGCCC GACGAACTCA GTCACCCCGC CAGACCCCCC 
TCCGAACCCA AATCCGGCGC ATTCCCCAGG AAGCTCTGGC AACCCGAGGC CGGTGAGGCT 
ACCCCGAGGT CCCCGCAGCC TGAGTTGAGT ACCTTTCCCA AGAAGCCTGC GCAGCCTGAG 
TTCAACGTGT ACCCCAAAAA GCCTCCGCAG CCTCAGGTCG GTGGCCTCCC TAAGAAGTCC 
GTGCCGCAGC CTGAGTTCAG CGAGGCCGCT CAGACTCCCC TCTGGAAGCC TCAGTCCAGC 
GAGCCGAAGC GCGACTCCAG CGCCTTTCCC AAAAAGGCCT CCCAGCCTCC GCTGAGTGAC 
TTTCCCAAGA AGCCTCCGCA GCCTGAGCTT GGGGACCTCA CCAGGACCTC CTCAGAGCCC 
GAAGTCAGCG TGCTTCCCAA GAGGCCGCGG CCGGCCGAAT TCAAAGCGCT CTCCAAGAAG 
CCCCCGCAGC CCGAGCTGGG CGGCCTCCCC AGGACCTCCT CAGAGCCCGA GTTCAACTCA 
CTCCCCAGGA AGCTGCTGCA GCCGGAGCGC CGGGGGCCAC CCCGCAAGTT CTCACAGCCT 
GAGCCCAGCG CTGTCCTCAA GAGACACCCG CAGCCTGAGT TCTTCGGTGA TCTCCCTCGA 
AAGCCTCCAC TCCCCAGCTC CGCTTCCGAG AGCTCACTGC CTGCGGCCGT GGCCGGCTTC 
AGCTCCCGGC ACCCGCTCAG CCCTGGGTTT GGAGCGGCTG GGACACCCCG CTGGAGGTCA 
GGAGGCCTGG TTCACAGTGG AGGGGCCAGG CCAGGCCTCA GACCCAGCCA TCCACCCCGG 
CGGAGGCCTC TGCCCCCTGC CAGCAGCCTG GGACACCCTC CAGCCAAGCC CCCGCTGCCC 
CCGGGGCCCG TGGATATGCA GAGCTTTCGG AGACCCTCTG CAGCATCCAT AGATCTACGG 
AGGACCCGCT CGGCCGCTGG GCTCCACTTC CAGGACCGAC AGCCTGAAGA CATCCCGCAG 
GTCCCAGATG AGATCTACGA GCTGTATGAC GATGTGGAAC CCAGAGATGA CTCCAGCCCC 
AGCCCCAAGG GCAGAGATGA AGCGCCCTCA GTTCAGCAAG CCGCCAGGAG GCCACCACAA 
GACCCAGCGC TCAGGAAGGA GAAGGATCCC CAGCCACAGC AGTTGCCACC CATGGACCCA 
AAGTTGCTGA AGCAGCTGAG GAAGGCAGAG AAGGCCGAGA GGGAGTTCCG GAAGAAGTTC 
AAGTTTGAAG GGGAGATCGT GGTTCACACG AAGATGATGA TCGACCCCAA CGCTAAGACA 
CGTCGCGGGG GTGGCAAGCA CCTCGGGATC CGGCGCGGGG AGATCCTGGA GGTGATCGAG 
TTCACCAGCA ATGAGGAGAT GCTGTGCCGG GACCCCAAAG GCAAATATGG CTACGTGCCC 
AGAACAGCGC TCCTGCCCCT GGAGACGGAG GTGTACGATG ATGTCGACTT CTGCGGTACG 
TGGACACTCT GGGAGGGCTG GAGGGACCCT GGCGATTCCC CGGGGGCGCT GCTCACTATT 
GGGCTTCTGG GGAGAGGGGT CCATGGAAGT CACGCTTCCT CGGCAGATCC CCTGGAAAAC 
CAACCACTCC CCCTGGGACG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005272559.1
CDS	50..2173
Translation	MAHHLPAAMESHQDFRSIKAKFQASQPEPSDLPKKPPKPEFGKLKKFSQPELSEHPKKAPLPEFGAVSLKPPPPEVTDLPKKPPPPEVTDLPKKPPPPEVTDLPKKPPPPEVTDLPKKPSKLELSDLSKKFPQLGATPFPRKPLQPEVGEAPLKASLPEPGAPARKPLQPDELSHPARPPSEPKSGAFPRKLWQPEAGEATPRSPQPELSTFPKKPAQPEFNVYPKKPPQPQVGGLPKKSVPQPEFSEAAQTPLWKPQSSEPKRDSSAFPKKASQPPLSDFPKKPPQPELGDLTRTSSEPEVSVLPKRPRPAEFKALSKKPPQPELGGLPRTSSEPEFNSLPRKLLQPERRGPPRKFSQPEPSAVLKRHPQPEFFGDLPRKPPLPSSASESSLPAAVAGFSSRHPLSPGFGAAGTPRWRSGGLVHSGGARPGLRPSHPPRRRPLPPASSLGHPPAKPPLPPGPVDMQSFRRPSAASIDLRRTRSAAGLHFQDRQPEDIPQVPDEIYELYDDVEPRDDSSPSPKGRDEAPSVQQAARRPPQDPALRKEKDPQPQQLPPMDPKLLKQLRKAEKAEREFRKKFKFEGEIVVHTKMMIDPNAKTRRGGGKHLGIRRGEILEVIEFTSNEEMLCRDPKGKYGYVPRTALLPLETEVYDDVDFCGTWTLWEGWRDPGDSPGALLTIGLLGRGVHGSHASSADPLENQPLPLGR

Target ORF information:

RefSeq Version	XM_005272502.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PML-RARA regulated adaptor molecule 1 (PRAM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005272502.2

ORF Insert Sequence:

ATGGCCCATC ACCTGCCTGC AGCCATGGAG AGCCATCAGG ACTTCCGGAG CATCAAAGCA 
AAGTTCCAGG CCTCTCAGCC GGAGCCCAGC GACCTGCCCA AAAAACCTCC GAAGCCTGAG 
TTTGGTAAAC TGAAGAAGTT CTCCCAGCCT GAGCTAAGCG AGCACCCCAA GAAGGCCCCG 
CTGCCTGAGT TTGGTGCAGT GTCCTTGAAG CCCCCGCCGC CTGAGGTCAC TGACCTCCCC 
AAGAAGCCCC CGCCGCCTGA GGTCACTGAC CTCCCCAAGA AGCCCCCGCC GCCTGAGGTC 
ACTGACCTCC CCAAGAAGCC CCCGCCGCCT GAGGTCACTG ACCTCCCCAA GAAGCCGTCC 
AAACTGGAGT TGAGTGACCT CTCCAAGAAG TTCCCACAGC TGGGGGCCAC TCCGTTTCCA 
AGGAAGCCCC TGCAGCCTGA GGTCGGTGAG GCCCCTTTGA AGGCCTCGCT GCCGGAGCCT 
GGTGCGCCGG CCCGGAAACC CCTGCAGCCC GACGAACTCA GTCACCCCGC CAGACCCCCC 
TCCGAACCCA AATCCGGCGC ATTCCCCAGG AAGCTCTGGC AACCCGAGGC CGGTGAGGCT 
ACCCCGAGGT CCCCGCAGCC TGAGTTGAGT ACCTTTCCCA AGAAGCCTGC GCAGCCTGAG 
TTCAACGTGT ACCCCAAAAA GCCTCCGCAG CCTCAGGTCG GTGGCCTCCC TAAGAAGTCC 
GTGCCGCAGC CTGAGTTCAG CGAGGCCGCT CAGACTCCCC TCTGGAAGCC TCAGTCCAGC 
GAGCCGAAGC GCGACTCCAG CGCCTTTCCC AAAAAGGCCT CCCAGCCTCC GCTGAGTGAC 
TTTCCCAAGA AGCCTCCGCA GCCTGAGCTT GGGGACCTCA CCAGGACCTC CTCAGAGCCC 
GAAGTCAGCG TGCTTCCCAA GAGGCCGCGG CCGGCCGAAT TCAAAGCGCT CTCCAAGAAG 
CCCCCGCAGC CCGAGCTGGG CGGCCTCCCC AGGACCTCCT CAGAGCCCGA GTTCAACTCA 
CTCCCCAGGA AGCTGCTGCA GCCGGAGCGC CGGGGGCCAC CCCGCAAGTT CTCACAGCCT 
GAGCCCAGCG CTGTCCTCAA GAGACACCCG CAGCCTGAGT TCTTCGGTGA TCTCCCTCGA 
AAGCCTCCAC TCCCCAGCTC CGCTTCCGAG AGCTCACTGC CTGCGGCCGT GGCCGGCTTC 
AGCTCCCGGC ACCCGCTCAG CCCTGGGTTT GGAGCGGCTG GGACACCCCG CTGGAGGTCA 
GGAGGCCTGG TTCACAGTGG AGGGGCCAGG CCAGGCCTCA GACCCAGCCA TCCACCCCGG 
CGGAGGCCTC TGCCCCCTGC CAGCAGCCTG GGACACCCTC CAGCCAAGCC CCCGCTGCCC 
CCGGGGCCCG TGGATATGCA GAGCTTTCGG AGACCCTCTG CAGCATCCAT AGATCTACGG 
AGGACCCGCT CGGCCGCTGG GCTCCACTTC CAGGACCGAC AGCCTGAAGA CATCCCGCAG 
GTCCCAGATG AGATCTACGA GCTGTATGAC GATGTGGAAC CCAGAGATGA CTCCAGCCCC 
AGCCCCAAGG GCAGAGATGA AGCGCCCTCA GTTCAGCAAG CCGCCAGGAG GCCACCACAA 
GACCCAGCGC TCAGGAAGGA GAAGGATCCC CAGCCACAGC AGTTGCCACC CATGGACCCA 
AAGTTGCTGA AGCAGCTGAG GAAGGCAGAG AAGGCCGAGA GGGAGTTCCG GAAGAAGTTC 
AAGTTTGAAG GGGAGATCGT GGTTCACACG AAGATGATGA TCGACCCCAA CGCTAAGACA 
CGTCGCGGGG GTGGCAAGCA CCTCGGGATC CGGCGCGGGG AGATCCTGGA GGTGATCGAG 
TTCACCAGCA ATGAGGAGAT GCTGTGCCGG GACCCCAAAG GCAAATATGG CTACGTGCCC 
AGAACAGCGC TCCTGCCCCT GGAGACGGAG GTGTACGATG ATGTCGACTT CTGCGGTACG 
TGGACACTCT GGGAGGGCTG GAGGGACCCT GGCGATTCCC CGGGGGCGCT GCTCACTATT 
GGGCTTCTGG GGAGAGGGGT CCATGGAAGT CACGCTTCCT CGGCAGATCC CCTGGAAAAC 
CAACCACTCC CCCTGGGACG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRAM1 ( XM_011528352.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526654.1 Homo sapiens PML-RARA regulated adaptor molecule 1 (PRAM1), transcript variant X1, mRNA.

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CloneID	OHu73332
Clone ID Related Accession (Same CDS sequence)	XM_011528352.2
Accession Version	XM_011528352.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	PML-RARA-regulated adapter molecule 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528352.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACGAG ACTGGAGACA TGCAGTGGGA GGGGAGAGCC ATCAGGACTT CCGGAGCATC 
AAAGCAAAGT TCCAGGCCTC TCAGCCGGAG CCCAGCGACC TGCCCAAAAA ACCTCCGAAG 
CCTGAGTTTG GTAAACTGAA GAAGTTCTCC CAGCCTGAGC TAAGCGAGCA CCCCAAGAAG 
GCCCCGCTGC CTGAGTTTGG TGCAGTGTCC TTGAAGCCCC CGCCGCCTGA GGTCACTGAC 
CTCCCCAAGA AGCCCCCGCC GCCTGAGGTC ACTGACCTCC CCAAGAAGCC CCCGCCGCCT 
GAGGTCACTG ACCTCCCCAA GAAGCCCCCG CCGCCTGAGG TCACTGACCT CCCCAAGAAG 
CCGTCCAAAC TGGAGTTGAG TGACCTCTCC AAGAAGTTCC CACAGCTGGG GGCCACTCCG 
TTTCCAAGGA AGCCCCTGCA GCCTGAGGTC GGTGAGGCCC CTTTGAAGGC CTCGCTGCCG 
GAGCCTGGTG CGCCGGCCCG GAAACCCCTG CAGCCCGACG AACTCAGTCA CCCCGCCAGA 
CCCCCCTCCG AACCCAAATC CGGCGCATTC CCCAGGAAGC TCTGGCAACC CGAGGCCGGT 
GAGGCTACCC CGAGGTCCCC GCAGCCTGAG TTGAGTACCT TTCCCAAGAA GCCTGCGCAG 
CCTGAGTTCA ACGTGTACCC CAAAAAGCCT CCGCAGCCTC AGGTCGGTGG CCTCCCTAAG 
AAGTCCGTGC CGCAGCCTGA GTTCAGCGAG GCCGCTCAGA CTCCCCTCTG GAAGCCTCAG 
TCCAGCGAGC CGAAGCGCGA CTCCAGCGCC TTTCCCAAAA AGGCCTCCCA GCCTCCGCTG 
AGTGACTTTC CCAAGAAGCC TCCGCAGCCT GAGCTTGGGG ACCTCACCAG GACCTCCTCA 
GAGCCCGAAG TCAGCGTGCT TCCCAAGAGG CCGCGGCCGG CCGAATTCAA AGCGCTCTCC 
AAGAAGCCCC CGCAGCCCGA GCTGGGCGGC CTCCCCAGGA CCTCCTCAGA GCCCGAGTTC 
AACTCACTCC CCAGGAAGCT GCTGCAGCCG GAGCGCCGGG GGCCACCCCG CAAGTTCTCA 
CAGCCTGAGC CCAGCGCTGT CCTCAAGAGA CACCCGCAGC CTGAGTTCTT CGGTGATCTC 
CCTCGAAAGC CTCCACTCCC CAGCTCCGCT TCCGAGAGCT CACTGCCTGC GGCCGTGGCC 
GGCTTCAGCT CCCGGCACCC GCTCAGCCCT GGGTTTGGAG CGGCTGGGAC ACCCCGCTGG 
AGGTCAGGAG GCCTGGTTCA CAGTGGAGGG GCCAGGCCAG GCCTCAGACC CAGCCATCCA 
CCCCGGCGGA GGCCTCTGCC CCCTGCCAGC AGCCTGGGAC ACCCTCCAGC CAAGCCCCCG 
CTGCCCCCGG GGCCCGTGGA TATGCAGAGC TTTCGGAGAC CCTCTGCAGC ATCCATAGAT 
CTACGGAGGA CCCGCTCGGC CGCTGGGCTC CACTTCCAGG ACCGACAGCC TGAAGACATC 
CCGCAGGTCC CAGATGAGAT CTACGAGCTG TATGACGATG TGGAACCCAG AGATGACTCC 
AGCCCCAGCC CCAAGGGCAG AGATGAAGCG CCCTCAGTTC AGCAAGCCGC CAGGAGGCCA 
CCACAAGACC CAGCGCTCAG GAAGGAGAAG GATCCCCAGC CACAGCAGTT GCCACCCATG 
GACCCAAAGT TGCTGAAGCA GCTGAGGAAG GCAGAGAAGG CCGAGAGGGA GTTCCGGAAG 
AAGTTCAAGT TTGAAGGGGA GATCGTGGTT CACACGAAGA TGATGATCGA CCCCAACGCT 
AAGACACGTC GCGGGGGTGG CAAGCACCTC GGGATCCGGC GCGGGGAGAT CCTGGAGGTG 
ATCGAGTTCA CCAGCAATGA GGAGATGCTG TGCCGGGACC CCAAAGGCAA ATATGGCTAC 
GTGCCCAGAA CAGCGCTCCT GCCCCTGGAG ACGGAGGTGT ACGATGATGT CGACTTCTGC 
GGTACGTGGA CACTCTGGGA GGGCTGGAGG GACCCTGGCG ATTCCCCGGG GGCGCTGCTC 
ACTATTGGGC TTCTGGGGAG AGGGGTCCAT GGAAGTCACG CTTCCTCGGC AGATCCCCTG 
GAAAACCAAC CACTCCCCCT GGGACGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526654.1
CDS	64..2193
Translation	MERDWRHAVGGESHQDFRSIKAKFQASQPEPSDLPKKPPKPEFGKLKKFSQPELSEHPKKAPLPEFGAVSLKPPPPEVTDLPKKPPPPEVTDLPKKPPPPEVTDLPKKPPPPEVTDLPKKPSKLELSDLSKKFPQLGATPFPRKPLQPEVGEAPLKASLPEPGAPARKPLQPDELSHPARPPSEPKSGAFPRKLWQPEAGEATPRSPQPELSTFPKKPAQPEFNVYPKKPPQPQVGGLPKKSVPQPEFSEAAQTPLWKPQSSEPKRDSSAFPKKASQPPLSDFPKKPPQPELGDLTRTSSEPEVSVLPKRPRPAEFKALSKKPPQPELGGLPRTSSEPEFNSLPRKLLQPERRGPPRKFSQPEPSAVLKRHPQPEFFGDLPRKPPLPSSASESSLPAAVAGFSSRHPLSPGFGAAGTPRWRSGGLVHSGGARPGLRPSHPPRRRPLPPASSLGHPPAKPPLPPGPVDMQSFRRPSAASIDLRRTRSAAGLHFQDRQPEDIPQVPDEIYELYDDVEPRDDSSPSPKGRDEAPSVQQAARRPPQDPALRKEKDPQPQQLPPMDPKLLKQLRKAEKAEREFRKKFKFEGEIVVHTKMMIDPNAKTRRGGGKHLGIRRGEILEVIEFTSNEEMLCRDPKGKYGYVPRTALLPLETEVYDDVDFCGTWTLWEGWRDPGDSPGALLTIGLLGRGVHGSHASSADPLENQPLPLGR

Target ORF information:

RefSeq Version	XM_011528352.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PML-RARA regulated adaptor molecule 1 (PRAM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528352.2

ORF Insert Sequence:

ATGGAACGAG ACTGGAGACA TGCAGTGGGA GGGGAGAGCC ATCAGGACTT CCGGAGCATC 
AAAGCAAAGT TCCAGGCCTC TCAGCCGGAG CCCAGCGACC TGCCCAAAAA ACCTCCGAAG 
CCTGAGTTTG GTAAACTGAA GAAGTTCTCC CAGCCTGAGC TAAGCGAGCA CCCCAAGAAG 
GCCCCGCTGC CTGAGTTTGG TGCAGTGTCC TTGAAGCCCC CGCCGCCTGA GGTCACTGAC 
CTCCCCAAGA AGCCCCCGCC GCCTGAGGTC ACTGACCTCC CCAAGAAGCC CCCGCCGCCT 
GAGGTCACTG ACCTCCCCAA GAAGCCCCCG CCGCCTGAGG TCACTGACCT CCCCAAGAAG 
CCGTCCAAAC TGGAGTTGAG TGACCTCTCC AAGAAGTTCC CACAGCTGGG GGCCACTCCG 
TTTCCAAGGA AGCCCCTGCA GCCTGAGGTC GGTGAGGCCC CTTTGAAGGC CTCGCTGCCG 
GAGCCTGGTG CGCCGGCCCG GAAACCCCTG CAGCCCGACG AACTCAGTCA CCCCGCCAGA 
CCCCCCTCCG AACCCAAATC CGGCGCATTC CCCAGGAAGC TCTGGCAACC CGAGGCCGGT 
GAGGCTACCC CGAGGTCCCC GCAGCCTGAG TTGAGTACCT TTCCCAAGAA GCCTGCGCAG 
CCTGAGTTCA ACGTGTACCC CAAAAAGCCT CCGCAGCCTC AGGTCGGTGG CCTCCCTAAG 
AAGTCCGTGC CGCAGCCTGA GTTCAGCGAG GCCGCTCAGA CTCCCCTCTG GAAGCCTCAG 
TCCAGCGAGC CGAAGCGCGA CTCCAGCGCC TTTCCCAAAA AGGCCTCCCA GCCTCCGCTG 
AGTGACTTTC CCAAGAAGCC TCCGCAGCCT GAGCTTGGGG ACCTCACCAG GACCTCCTCA 
GAGCCCGAAG TCAGCGTGCT TCCCAAGAGG CCGCGGCCGG CCGAATTCAA AGCGCTCTCC 
AAGAAGCCCC CGCAGCCCGA GCTGGGCGGC CTCCCCAGGA CCTCCTCAGA GCCCGAGTTC 
AACTCACTCC CCAGGAAGCT GCTGCAGCCG GAGCGCCGGG GGCCACCCCG CAAGTTCTCA 
CAGCCTGAGC CCAGCGCTGT CCTCAAGAGA CACCCGCAGC CTGAGTTCTT CGGTGATCTC 
CCTCGAAAGC CTCCACTCCC CAGCTCCGCT TCCGAGAGCT CACTGCCTGC GGCCGTGGCC 
GGCTTCAGCT CCCGGCACCC GCTCAGCCCT GGGTTTGGAG CGGCTGGGAC ACCCCGCTGG 
AGGTCAGGAG GCCTGGTTCA CAGTGGAGGG GCCAGGCCAG GCCTCAGACC CAGCCATCCA 
CCCCGGCGGA GGCCTCTGCC CCCTGCCAGC AGCCTGGGAC ACCCTCCAGC CAAGCCCCCG 
CTGCCCCCGG GGCCCGTGGA TATGCAGAGC TTTCGGAGAC CCTCTGCAGC ATCCATAGAT 
CTACGGAGGA CCCGCTCGGC CGCTGGGCTC CACTTCCAGG ACCGACAGCC TGAAGACATC 
CCGCAGGTCC CAGATGAGAT CTACGAGCTG TATGACGATG TGGAACCCAG AGATGACTCC 
AGCCCCAGCC CCAAGGGCAG AGATGAAGCG CCCTCAGTTC AGCAAGCCGC CAGGAGGCCA 
CCACAAGACC CAGCGCTCAG GAAGGAGAAG GATCCCCAGC CACAGCAGTT GCCACCCATG 
GACCCAAAGT TGCTGAAGCA GCTGAGGAAG GCAGAGAAGG CCGAGAGGGA GTTCCGGAAG 
AAGTTCAAGT TTGAAGGGGA GATCGTGGTT CACACGAAGA TGATGATCGA CCCCAACGCT 
AAGACACGTC GCGGGGGTGG CAAGCACCTC GGGATCCGGC GCGGGGAGAT CCTGGAGGTG 
ATCGAGTTCA CCAGCAATGA GGAGATGCTG TGCCGGGACC CCAAAGGCAA ATATGGCTAC 
GTGCCCAGAA CAGCGCTCCT GCCCCTGGAG ACGGAGGTGT ACGATGATGT CGACTTCTGC 
GGTACGTGGA CACTCTGGGA GGGCTGGAGG GACCCTGGCG ATTCCCCGGG GGCGCTGCTC 
ACTATTGGGC TTCTGGGGAG AGGGGTCCAT GGAAGTCACG CTTCCTCGGC AGATCCCCTG 
GAAAACCAAC CACTCCCCCT GGGACGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRAM1 ( XM_011528353.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526655.1 Homo sapiens PML-RARA regulated adaptor molecule 1 (PRAM1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu73333
Clone ID Related Accession (Same CDS sequence)	XM_011528353.2
Accession Version	XM_011528353.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	PML-RARA-regulated adapter molecule 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528353.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACGAG ACTGGAGACA TGCAGTGGGA GGGGAGAGCC ATCAGGACTT CCGGAGCATC 
AAAGCAAAGT TCCAGGCCTC TCAGCCGGAG CCCAGCGACC TGCCCAAAAA ACCTCCGAAG 
CCTGAGTTTG GTAAACTGAA GAAGTTCTCC CAGCCTGAGC TAAGCGAGCA CCCCAAGAAG 
GCCCCGCTGC CTGAGTTTGG TGCAGTGTCC TTGAAGCCCC CGCCGCCTGA GGTCACTGAC 
CTCCCCAAGA AGCCCCCGCC GCCTGAGGTC ACTGACCTCC CCAAGAAGCC CCCGCCGCCT 
GAGGTCACTG ACCTCCCCAA GAAGCCCCCG CCGCCTGAGG TCACTGACCT CCCCAAGAAG 
CCGTCCAAAC TGGAGTTGAG TGACCTCTCC AAGAAGTTCC CACAGCTGGG GGCCACTCCG 
TTTCCAAGGA AGCCCCTGCA GCCTGAGGTC GGTGAGGCCC CTTTGAAGGC CTCGCTGCCG 
GAGCCTGGTG CGCCGGCCCG GAAACCCCTG CAGCCCGACG AACTCAGTCA CCCCGCCAGA 
CCCCCCTCCG AACCCAAATC CGGCGCATTC CCCAGGAAGC TCTGGCAACC CGAGGCCGGT 
GAGGCTACCC CGAGGTCCCC GCAGCCTGAG TTGAGTACCT TTCCCAAGAA GCCTGCGCAG 
CCTGAGTTCA ACGTGTACCC CAAAAAGCCT CCGCAGCCTC AGGTCGGTGG CCTCCCTAAG 
AAGTCCGTGC CGCAGCCTGA GTTCAGCGAG GCCGCTCAGA CTCCCCTCTG GAAGCCTCAG 
TCCAGCGAGC CGAAGCGCGA CTCCAGCGCC TTTCCCAAAA AGGCCTCCCA GCCTCCGCTG 
AGTGACTTTC CCAAGAAGCC TCCGCAGCCT GAGCTTGGGG ACCTCACCAG GACCTCCTCA 
GAGCCCGAAG TCAGCGTGCT TCCCAAGAGG CCGCGGCCGG CCGAATTCAA AGCGCTCTCC 
AAGAAGCCCC CGCAGCCCGA GCTGGGCGGC CTCCCCAGGA CCTCCTCAGA GCCCGAGTTC 
AACTCACTCC CCAGGAAGCT GCTGCAGCCG GAGCGCCGGG GGCCACCCCG CAAGTTCTCA 
CAGCCTGAGC CCAGCGCTGT CCTCAAGAGA CACCCGCAGC CTGAGTTCTT CGGTGATCTC 
CCTCGAAAGC CTCCACTCCC CAGCTCCGCT TCCGAGAGCT CACTGCCTGC GGCCGTGGCC 
GGCTTCAGCT CCCGGCACCC GCTCAGCCCT GGGTTTGGAG CGGCTGGGAC ACCCCGCTGG 
AGGTCAGGAG GCCTGGTTCA CAGTGGAGGG GCCAGGCCAG GCCTCAGACC CAGCCATCCA 
CCCCGGCGGA GGCCTCTGCC CCCTGCCAGC AGCCTGGGAC ACCCTCCAGC CAAGCCCCCG 
CTGCCCCCGG GGCCCGTGGA TATGCAGAGC TTTCGGAGAC CCTCTGCAGC ATCCATAGAT 
CTACGGAGGA CCCGCTCGGC CGCTGGGCTC CACTTCCAGG ACCGACAGCC TGAAGACATC 
CCGCAGGTCC CAGATGAGAT CTACGAGCTG TATGACGATG TGGAACCCAG AGATGACTCC 
AGCCCCAGCC CCAAGGGCAG AGATGAAGCG CCCTCAGTTC AGCAAGCCGC CAGGAGGCCA 
CCACAAGACC CAGCGCTCAG GAAGGAGAAG GATCCCCAGC CACAGCAGTT GCCACCCATG 
GACCCAAAGT TGCTGAAGCA GCTGAGGAAG GCAGAGAAGG CCGAGAGGGA GTTCCGGAAG 
AAGTTCAAGT TTGAAGGGGA GATCGTGGTT CACACGAAGA TGATGATCGA CCCCAACGCT 
AAGACACGTC GCGGGGGTGG CAAGCACCTC GGGATCCGGC GCGGGGAGAT CCTGGAGGTG 
ATCGAGTTCA CCAGCAATGA GGAGATGCTG TGCCGGGACC CCAAAGGCAA ATATGGCTAC 
GTGCCCAGAA CAGCGCTCCT GCCCCTGGAG ACGGAGGTGT ACGATGATGT CGACTTCTGC 
GATCCCCTGG AAAACCAACC ACTCCCCCTG GGACGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526655.1
CDS	62..2080
Translation	MERDWRHAVGGESHQDFRSIKAKFQASQPEPSDLPKKPPKPEFGKLKKFSQPELSEHPKKAPLPEFGAVSLKPPPPEVTDLPKKPPPPEVTDLPKKPPPPEVTDLPKKPPPPEVTDLPKKPSKLELSDLSKKFPQLGATPFPRKPLQPEVGEAPLKASLPEPGAPARKPLQPDELSHPARPPSEPKSGAFPRKLWQPEAGEATPRSPQPELSTFPKKPAQPEFNVYPKKPPQPQVGGLPKKSVPQPEFSEAAQTPLWKPQSSEPKRDSSAFPKKASQPPLSDFPKKPPQPELGDLTRTSSEPEVSVLPKRPRPAEFKALSKKPPQPELGGLPRTSSEPEFNSLPRKLLQPERRGPPRKFSQPEPSAVLKRHPQPEFFGDLPRKPPLPSSASESSLPAAVAGFSSRHPLSPGFGAAGTPRWRSGGLVHSGGARPGLRPSHPPRRRPLPPASSLGHPPAKPPLPPGPVDMQSFRRPSAASIDLRRTRSAAGLHFQDRQPEDIPQVPDEIYELYDDVEPRDDSSPSPKGRDEAPSVQQAARRPPQDPALRKEKDPQPQQLPPMDPKLLKQLRKAEKAEREFRKKFKFEGEIVVHTKMMIDPNAKTRRGGGKHLGIRRGEILEVIEFTSNEEMLCRDPKGKYGYVPRTALLPLETEVYDDVDFCDPLENQPLPLGR

Target ORF information:

RefSeq Version	XM_011528353.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PML-RARA regulated adaptor molecule 1 (PRAM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528353.2

ORF Insert Sequence:

ATGGAACGAG ACTGGAGACA TGCAGTGGGA GGGGAGAGCC ATCAGGACTT CCGGAGCATC 
AAAGCAAAGT TCCAGGCCTC TCAGCCGGAG CCCAGCGACC TGCCCAAAAA ACCTCCGAAG 
CCTGAGTTTG GTAAACTGAA GAAGTTCTCC CAGCCTGAGC TAAGCGAGCA CCCCAAGAAG 
GCCCCGCTGC CTGAGTTTGG TGCAGTGTCC TTGAAGCCCC CGCCGCCTGA GGTCACTGAC 
CTCCCCAAGA AGCCCCCGCC GCCTGAGGTC ACTGACCTCC CCAAGAAGCC CCCGCCGCCT 
GAGGTCACTG ACCTCCCCAA GAAGCCCCCG CCGCCTGAGG TCACTGACCT CCCCAAGAAG 
CCGTCCAAAC TGGAGTTGAG TGACCTCTCC AAGAAGTTCC CACAGCTGGG GGCCACTCCG 
TTTCCAAGGA AGCCCCTGCA GCCTGAGGTC GGTGAGGCCC CTTTGAAGGC CTCGCTGCCG 
GAGCCTGGTG CGCCGGCCCG GAAACCCCTG CAGCCCGACG AACTCAGTCA CCCCGCCAGA 
CCCCCCTCCG AACCCAAATC CGGCGCATTC CCCAGGAAGC TCTGGCAACC CGAGGCCGGT 
GAGGCTACCC CGAGGTCCCC GCAGCCTGAG TTGAGTACCT TTCCCAAGAA GCCTGCGCAG 
CCTGAGTTCA ACGTGTACCC CAAAAAGCCT CCGCAGCCTC AGGTCGGTGG CCTCCCTAAG 
AAGTCCGTGC CGCAGCCTGA GTTCAGCGAG GCCGCTCAGA CTCCCCTCTG GAAGCCTCAG 
TCCAGCGAGC CGAAGCGCGA CTCCAGCGCC TTTCCCAAAA AGGCCTCCCA GCCTCCGCTG 
AGTGACTTTC CCAAGAAGCC TCCGCAGCCT GAGCTTGGGG ACCTCACCAG GACCTCCTCA 
GAGCCCGAAG TCAGCGTGCT TCCCAAGAGG CCGCGGCCGG CCGAATTCAA AGCGCTCTCC 
AAGAAGCCCC CGCAGCCCGA GCTGGGCGGC CTCCCCAGGA CCTCCTCAGA GCCCGAGTTC 
AACTCACTCC CCAGGAAGCT GCTGCAGCCG GAGCGCCGGG GGCCACCCCG CAAGTTCTCA 
CAGCCTGAGC CCAGCGCTGT CCTCAAGAGA CACCCGCAGC CTGAGTTCTT CGGTGATCTC 
CCTCGAAAGC CTCCACTCCC CAGCTCCGCT TCCGAGAGCT CACTGCCTGC GGCCGTGGCC 
GGCTTCAGCT CCCGGCACCC GCTCAGCCCT GGGTTTGGAG CGGCTGGGAC ACCCCGCTGG 
AGGTCAGGAG GCCTGGTTCA CAGTGGAGGG GCCAGGCCAG GCCTCAGACC CAGCCATCCA 
CCCCGGCGGA GGCCTCTGCC CCCTGCCAGC AGCCTGGGAC ACCCTCCAGC CAAGCCCCCG 
CTGCCCCCGG GGCCCGTGGA TATGCAGAGC TTTCGGAGAC CCTCTGCAGC ATCCATAGAT 
CTACGGAGGA CCCGCTCGGC CGCTGGGCTC CACTTCCAGG ACCGACAGCC TGAAGACATC 
CCGCAGGTCC CAGATGAGAT CTACGAGCTG TATGACGATG TGGAACCCAG AGATGACTCC 
AGCCCCAGCC CCAAGGGCAG AGATGAAGCG CCCTCAGTTC AGCAAGCCGC CAGGAGGCCA 
CCACAAGACC CAGCGCTCAG GAAGGAGAAG GATCCCCAGC CACAGCAGTT GCCACCCATG 
GACCCAAAGT TGCTGAAGCA GCTGAGGAAG GCAGAGAAGG CCGAGAGGGA GTTCCGGAAG 
AAGTTCAAGT TTGAAGGGGA GATCGTGGTT CACACGAAGA TGATGATCGA CCCCAACGCT 
AAGACACGTC GCGGGGGTGG CAAGCACCTC GGGATCCGGC GCGGGGAGAT CCTGGAGGTG 
ATCGAGTTCA CCAGCAATGA GGAGATGCTG TGCCGGGACC CCAAAGGCAA ATATGGCTAC 
GTGCCCAGAA CAGCGCTCCT GCCCCTGGAG ACGGAGGTGT ACGATGATGT CGACTTCTGC 
GATCCCCTGG AAAACCAACC ACTCCCCCTG GGACGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Spinal promyelocytic sarcoma as the initial manifestation of acute promyelocytic leukaemia.
Neurologia (Barcelona, Spain)(2017 Apr)
Villase?or-Ledezma J,Amosa-Delgado M,Ru?z-Gin?s JA,?lvarez-Salgado JA

Regulation of NF-?B by PML and PML-RAR?.
Scientific reports744539(2017 Mar)
Ahmed A,Wan X,Mitxitorena I,Lindsay AJ,Paolo Pandolfi P,McCaffrey MW,Keeshan K,Chen YH,Carmody RJ

Absolute quantification of the pretreatment PML-RARA transcript defines the relapse risk in acute promyelocytic leukemia.
Oncotarget6(15)13269-77(2015 May)
Albano F,Zagaria A,Anelli L,Coccaro N,Tota G,Brunetti C,Minervini CF,Impera L,Minervini A,Cellamare A,Orsini P,Cumbo C,Casieri P,Specchia G

Development and validation of a 3-Plex RT-qPCR assay for the simultaneous detection and quantitation of the three PML-RARa fusion transcripts in acute promyelocytic leukemia.
PloS one10(3)e0122530(2015)
Chen Z,Tong Y,Li Y,Gao Q,Wang Q,Fu C,Xia Z

MIR125B1 represses the degradation of the PML-RARA oncoprotein by an autophagy-lysosomal pathway in acute promyelocytic leukemia.
Autophagy10(10)1726-37(2014 Oct)
Zeng CW,Chen ZH,Zhang XJ,Han BW,Lin KY,Li XJ,Wei PP,Zhang H,Li Y,Chen YQ

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PRAM1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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