Emilin3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Emilin3
Entrez Gene ID	280635
Full Name	elastin microfibril interfacer 3
Synonyms	1110013O17Rik,EMILIN-3,EMILIN-5,EMILIN-T,Emilin5
General protein information	Preferred Names elastin microfibril interfacer 3 Names EMILIN-3 elastin microfibril interface located protein 5 elastin microfibril interface-located protein 3 elastin microfibril interfacer 5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 H2

mRNA and Protein(s)

mRNA	Protein	Name
NM_182840.2	NP_878260.1	EMILIN-3 isoform a precursor
NM_001291145.1	NP_001278074.1	EMILIN-3 isoform b precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	emilin3a	XP_699102.3
Rattus norvegicus (Norway rat)	Emilin3	NP_001103371.1
	EMILIN3	XP_001086225.1
Bos taurus (cattle)	EMILIN3	XP_003586866.2
Mus musculus (house mouse)	Emilin3	NP_878260.1
Gallus gallus (chicken)	EMILIN3	XP_425694.4
Danio rerio (zebrafish)	LOC101882042	XP_005161982.1
Xenopus tropicalis (tropical clawed frog)	emilin3	XP_002932911.2
Homo sapiens (human)	EMILIN3	NP_443078.1
Pan troglodytes (chimpanzee)	EMILIN3	XP_514653.2
Canis lupus familiaris (dog)	EMILIN3	XP_543001.2

Gene Ontology
Bibliography

Related articles in PubMed

Altered feto-placental vascularization, feto-placental malperfusion and fetal growth restriction in mice with
Lacko LA, Hurtado R, Hinds S, Poulos MG, Butler JM, Stuhlmann H
Development (Cambridge, England)144(13)2469-2479(2017 07)

EMILIN3, an extracellular matrix molecule with restricted distribution in skin.
Corallo D, Schiavinato A, Bizzotto D, Milanetto M, Guljelmovic M, Keene DR, Sengle G, Braghetta P, Bonaldo P
Experimental dermatology26(5)435-438(2017 May)

Transcriptome of the inner circular smooth muscle of the developing mouse intestine: Evidence for regulation of visceral smooth muscle genes by the hedgehog target gene, cJun.
Gurdziel K, Vogt KR, Walton KD, Schneider GK, Gumucio DL
Developmental dynamics : an official publication of the American Association of Anatomists245(5)614-26(2016 May)

EMILIN-3, peculiar member of elastin microfibril interface-located protein (EMILIN) family, has distinct expression pattern, forms oligomeric assemblies, and serves as transforming growth factor ? (TGF-?) antagonist.
Schiavinato A, Becker AK, Zanetti M, Corallo D, Milanetto M, Bizzotto D, Bressan G, Guljelmovic M, Paulsson M, Wagener R, Braghetta P, Bonaldo P
The Journal of biological chemistry287(14)11498-515(2012 Mar)

Genomewide discovery and classification of candidate ovarian fertility genes in the mouse.
Gallardo TD, John GB, Shirley L, Contreras CM, Akbay EA, Haynie JM, Ward SE, Shidler MJ, Castrillon DH
Genetics177(1)179-94(2007 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

characterization of the expression and biochemical properties of EMILIN-3
Title: EMILIN-3, peculiar member of elastin microfibril interface-located protein (EMILIN) family, has distinct expression pattern, forms oligomeric assemblies, and serves as transforming growth factor ? (TGF-?) antagonist.

Transcripts highly enriched in ovary; gene identified as candidate female fertility factor.
Title: Genomewide discovery and classification of candidate ovarian fertility genes in the mouse.

The following Emilin3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Emilin3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu17158	NM_001291145.1 Latest version!	Mus musculus elastin microfibril interfacer 3 (Emilin3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu15514	NM_182840.2 Latest version!	Mus musculus elastin microfibril interfacer 3 (Emilin3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$447.30 $639.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Emilin3 ( NM_001291145.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001278074.1 Mus musculus elastin microfibril interfacer 3 (Emilin3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu17158
Clone ID Related Accession (Same CDS sequence)	NM_001291145.1
Accession Version	NM_001291145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Mus musculus(house mouse)
Product	EMILIN-3 isoform b precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY139070.1, BC057619.1 and W50635.1. On Mar 27, 2014 this sequence version replaced XM_006499686.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849376 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCCGCC GTCTGTCCGT GTGGCTGTGC ACCGTCGCGG CGCTGCTCTC GGGGGCGCAG 
GCCAAGGGCA CCCCTCTCCT CGCGCGGCCC GCACAGCCCA GTGCCTCCCG TTACAGCCTC 
TACACCACCG GATGGCGTCC GCGGCTGCGC CCGGGGCCAC ACAAGTCTCT CTGTGCCTAT 
GTGGTACACA GAAATGTGAC CTGCGTCCTA CAAGAAGGAG CAGAGAGCTA TATAAAGGCT 
GAGTACCGGA ACTGTGGGTG GGGACCCAAC TGCCCTTCAA CAGTCAGGTA CCGCACAGTG 
TTCAGACCCA GATACAAGAT TGGCTACAAG ACCGTGACAG ACCTGGCGTG GCGCTGCTGC 
CCTGGTCTTA CTGGGGAAAG CTGCCCTGAA CACCTCACAG ACCATGGAGC CACCCCACCC 
CACCAGGAGC CAGAGCCTCA GATTCCCTTG GGGCAGCTGG GCCCAGGTCC CAGGCCTTCT 
CCTTACAGCA GAGAGGCCCC GCGCCCCCGT GGCTTTGGGG TCATCCCCGA GGGTCTTGTG 
GCCCCAGAAG ACAGAGGCAG AGGACCACTC ATCCCTCCTC TGAGTGAGAT CCTGAGCAAG 
GTCACAGAGG TGAGCAACAC ATTACAGACC AAGGTGCAGC TGTTAGATGA GGTGCGTGGG 
CTGGCCCTGG GGCATGAGGC TCACCTGCAG CGGCTCCGGG AAGCCCCGCC ATCCCCGCTG 
ACCTCCTTAG CGCTCCTAGA GGAATACGTG GACCAGCGGC TGCAGCGACT CTGGGGGAGC 
CTGCTGGATG GCTTTGAGCA GAAGCTGCAA GGTGTCCAGA GCGAGTGTGA CCTTCGGGTG 
CAAGAGGTGC GGCAGCAATG TGAGGAGGGC CAGGCAGCCA GCCAGCGCTT GCATCAGAGC 
CTCGATGGCC GAGAGCTTGC CTTGCGTCGG GAGCTCTCCC AGCTGGGCAC TCAGCTGCAG 
GGCTTGACTC TGACTGGTGG GGGCACCTGC TGCAGCCAAC TGGCTTTGAT CAGTGCCCGG 
GTGGACAGCC TTGAGAGGAA CCTGCAGGCT GTCACTGAGA CCCAAGGTGG CCCGGGCACC 
CTGGCCGCGG ATGAGCTTGC GCGCCTCTCT GCAGCTATGC TTCAGGGAGG TGTGGACGGG 
CTGCTTGAAG GCCTTGAGAC TATCAATGGG ACAGAGAATG GAGCAAGGGG CTGCTGTCTG 
AGAATGGAGG TGGGGGGCTG GGGTGTCGGT GGCTTCGGGT CCACGCTGGA ACAGCGAGTG 
CAGAGCCTAG AGGAGCGGCT GGCAACTCTG ACCGGAGAGC TAAGCCCCGA GAGTGCCATA 
CCAGACAGAT CAGCGCGGCC GCTTGTTCAT TCAGAACTAG CAGTACTGGA ACAAAGGCTG 
GTGTCCCTGG AGACCTCGTG CACCCCAAGT ACCACCACAG CCATCCTGGA CAACCTCGTG 
GCAGAGGTGA AGGCCTGGCA GAGCCGGAGC GAGGCTCTCC TACACCAGGT GGCTCGTCAC 
ACTGCCCTGC TCCAACAGCT CAATGGCACG GTAGCTGAAG TCCAGGGGCA GCTGGCAGAA 
GGGACAGGCA GCTCACTGCA AGGGGAGATC ACTCTCTTGA AAGTCAACCT GAACTCAGTG 
AGCAAGTCAC TCACGGGCCT CAGCGACTCT GTCAGCCAGT ACTCTGATGC CTTCTCGGCT 
GCCAACACAT CCCTGGATGA ACGAGAACGA AGAGTGGAAG CCGAGGTCCA CACCATCCAG 
GAACAGATCA GCAGCCAGGG CTCACGGCTT CAGGCTGGCC ACAGGCAGGT CCTGAACCTG 
CGAGGGGAGC TGGAGCAACT CAAGGCTGGC ATGGCCAATG TGGCTAGAGG GTTGAGCCGC 
TGCCGGGACA CAGCCCAGGA ACTCCAGCAT ACAGTGGGCC ACTTTGACCA GAGGGTGGCC 
CAAGTGGAAG GAGCATGTGA GAGGTTGGGC CTGTTGGCAA CCCACCTGAA CAGTCTGCCT 
ACTGAGCAGC TGAGGTCCAG GGAGGGCCTG TGGGGCCACA TAGACAAGCT GAACCACACA 
TTGGCCCAGC ACACGCAGGA TATCGCCCGG CTCCGGGATG ACCTGTTGGA CTGCAGAGCT 
CAGCTGGCTG AGGTGAGGCC AGGGCGAGCT GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278074.1
CDS	169..2304
Translation	MGRRLSVWLCTVAALLSGAQAKGTPLLARPAQPSASRYSLYTTGWRPRLRPGPHKSLCAYVVHRNVTCVLQEGAESYIKAEYRNCGWGPNCPSTVRYRTVFRPRYKIGYKTVTDLAWRCCPGLTGESCPEHLTDHGATPPHQEPEPQIPLGQLGPGPRPSPYSREAPRPRGFGVIPEGLVAPEDRGRGPLIPPLSEILSKVTEVSNTLQTKVQLLDEVRGLALGHEAHLQRLREAPPSPLTSLALLEEYVDQRLQRLWGSLLDGFEQKLQGVQSECDLRVQEVRQQCEEGQAASQRLHQSLDGRELALRRELSQLGTQLQGLTLTGGGTCCSQLALISARVDSLERNLQAVTETQGGPGTLAADELARLSAAMLQGGVDGLLEGLETINGTENGARGCCLRMEVGGWGVGGFGSTLEQRVQSLEERLATLTGELSPESAIPDRSARPLVHSELAVLEQRLVSLETSCTPSTTTAILDNLVAEVKAWQSRSEALLHQVARHTALLQQLNGTVAEVQGQLAEGTGSSLQGEITLLKVNLNSVSKSLTGLSDSVSQYSDAFSAANTSLDERERRVEAEVHTIQEQISSQGSRLQAGHRQVLNLRGELEQLKAGMANVARGLSRCRDTAQELQHTVGHFDQRVAQVEGACERLGLLATHLNSLPTEQLRSREGLWGHIDKLNHTLAQHTQDIARLRDDLLDCRAQLAEVRPGRAD

Target ORF information:

RefSeq Version	NM_001291145.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus elastin microfibril interfacer 3 (Emilin3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291145.1

ORF Insert Sequence:

ATGGGCCGCC GTCTGTCCGT GTGGCTGTGC ACCGTCGCGG CGCTGCTCTC GGGGGCGCAG 
GCCAAGGGCA CCCCTCTCCT CGCGCGGCCC GCACAGCCCA GTGCCTCCCG TTACAGCCTC 
TACACCACCG GATGGCGTCC GCGGCTGCGC CCGGGGCCAC ACAAGTCTCT CTGTGCCTAT 
GTGGTACACA GAAATGTGAC CTGCGTCCTA CAAGAAGGAG CAGAGAGCTA TATAAAGGCT 
GAGTACCGGA ACTGTGGGTG GGGACCCAAC TGCCCTTCAA CAGTCAGGTA CCGCACAGTG 
TTCAGACCCA GATACAAGAT TGGCTACAAG ACCGTGACAG ACCTGGCGTG GCGCTGCTGC 
CCTGGTCTTA CTGGGGAAAG CTGCCCTGAA CACCTCACAG ACCATGGAGC CACCCCACCC 
CACCAGGAGC CAGAGCCTCA GATTCCCTTG GGGCAGCTGG GCCCAGGTCC CAGGCCTTCT 
CCTTACAGCA GAGAGGCCCC GCGCCCCCGT GGCTTTGGGG TCATCCCCGA GGGTCTTGTG 
GCCCCAGAAG ACAGAGGCAG AGGACCACTC ATCCCTCCTC TGAGTGAGAT CCTGAGCAAG 
GTCACAGAGG TGAGCAACAC ATTACAGACC AAGGTGCAGC TGTTAGATGA GGTGCGTGGG 
CTGGCCCTGG GGCATGAGGC TCACCTGCAG CGGCTCCGGG AAGCCCCGCC ATCCCCGCTG 
ACCTCCTTAG CGCTCCTAGA GGAATACGTG GACCAGCGGC TGCAGCGACT CTGGGGGAGC 
CTGCTGGATG GCTTTGAGCA GAAGCTGCAA GGTGTCCAGA GCGAGTGTGA CCTTCGGGTG 
CAAGAGGTGC GGCAGCAATG TGAGGAGGGC CAGGCAGCCA GCCAGCGCTT GCATCAGAGC 
CTCGATGGCC GAGAGCTTGC CTTGCGTCGG GAGCTCTCCC AGCTGGGCAC TCAGCTGCAG 
GGCTTGACTC TGACTGGTGG GGGCACCTGC TGCAGCCAAC TGGCTTTGAT CAGTGCCCGG 
GTGGACAGCC TTGAGAGGAA CCTGCAGGCT GTCACTGAGA CCCAAGGTGG CCCGGGCACC 
CTGGCCGCGG ATGAGCTTGC GCGCCTCTCT GCAGCTATGC TTCAGGGAGG TGTGGACGGG 
CTGCTTGAAG GCCTTGAGAC TATCAATGGG ACAGAGAATG GAGCAAGGGG CTGCTGTCTG 
AGAATGGAGG TGGGGGGCTG GGGTGTCGGT GGCTTCGGGT CCACGCTGGA ACAGCGAGTG 
CAGAGCCTAG AGGAGCGGCT GGCAACTCTG ACCGGAGAGC TAAGCCCCGA GAGTGCCATA 
CCAGACAGAT CAGCGCGGCC GCTTGTTCAT TCAGAACTAG CAGTACTGGA ACAAAGGCTG 
GTGTCCCTGG AGACCTCGTG CACCCCAAGT ACCACCACAG CCATCCTGGA CAACCTCGTG 
GCAGAGGTGA AGGCCTGGCA GAGCCGGAGC GAGGCTCTCC TACACCAGGT GGCTCGTCAC 
ACTGCCCTGC TCCAACAGCT CAATGGCACG GTAGCTGAAG TCCAGGGGCA GCTGGCAGAA 
GGGACAGGCA GCTCACTGCA AGGGGAGATC ACTCTCTTGA AAGTCAACCT GAACTCAGTG 
AGCAAGTCAC TCACGGGCCT CAGCGACTCT GTCAGCCAGT ACTCTGATGC CTTCTCGGCT 
GCCAACACAT CCCTGGATGA ACGAGAACGA AGAGTGGAAG CCGAGGTCCA CACCATCCAG 
GAACAGATCA GCAGCCAGGG CTCACGGCTT CAGGCTGGCC ACAGGCAGGT CCTGAACCTG 
CGAGGGGAGC TGGAGCAACT CAAGGCTGGC ATGGCCAATG TGGCTAGAGG GTTGAGCCGC 
TGCCGGGACA CAGCCCAGGA ACTCCAGCAT ACAGTGGGCC ACTTTGACCA GAGGGTGGCC 
CAAGTGGAAG GAGCATGTGA GAGGTTGGGC CTGTTGGCAA CCCACCTGAA CAGTCTGCCT 
ACTGAGCAGC TGAGGTCCAG GGAGGGCCTG TGGGGCCACA TAGACAAGCT GAACCACACA 
TTGGCCCAGC ACACGCAGGA TATCGCCCGG CTCCGGGATG ACCTGTTGGA CTGCAGAGCT 
CAGCTGGCTG AGGTGAGGCC AGGGCGAGCT GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Emilin3 ( NM_182840.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_878260.1 Mus musculus elastin microfibril interfacer 3 (Emilin3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu15514
Clone ID Related Accession (Same CDS sequence)	NM_182840.2
Accession Version	NM_182840.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	EMILIN-3 isoform a precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY139070.1 and BC057619.1. On Mar 27, 2014 this sequence version replaced NM_182840.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: BC054738.1, BU512497.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCCGCC GTCTGTCCGT GTGGCTGTGC ACCGTCGCGG CGCTGCTCTC GGGGGCGCAG 
GCCAAGGGCA CCCCTCTCCT CGCGCGGCCC GCACAGCCCA GTGCCTCCCG TTACAGCCTC 
TACACCACCG GATGGCGTCC GCGGCTGCGC CCGGGGCCAC ACAAGTCTCT CTGTGCCTAT 
GTGGTACACA GAAATGTGAC CTGCGTCCTA CAAGAAGGAG CAGAGAGCTA TATAAAGGCT 
GAGTACCGGA ACTGTGGGTG GGGACCCAAC TGCCCTTCAA CAGTCAGGTA CCGCACAGTG 
TTCAGACCCA GATACAAGAT TGGCTACAAG ACCGTGACAG ACCTGGCGTG GCGCTGCTGC 
CCTGGTCTTA CTGGGGAAAG CTGCCCTGAA CACCTCACAG ACCATGGAGC CACCCCACCC 
CACCAGGAGC CAGAGCCTCA GATTCCCTTG GGGCAGCTGG GCCCAGGTCC CAGGCCTTCT 
CCTTACAGCA GAGAGGCCCC GCGCCCCCGT GGAAGAAAAG GCCAAGGGCC GTTTGGTGAG 
CGGCTAGAAC AGCGCCTGTC GCAAGCATAT GGCACCCTGA GTGGATTGGT AGCCAGCCAC 
GAGAATCCCA ATAGGATCAC TGGTGACTCC AGGGCCCCTG TCGTACCTAT AGGCTTTGGG 
GTCATCCCCG AGGGTCTTGT GGCCCCAGAA GACAGAGGCA GAGGACCACT CATCCCTCCT 
CTGAGTGAGA TCCTGAGCAA GGTCACAGAG GTGAGCAACA CATTACAGAC CAAGGTGCAG 
CTGTTAGATG AGGTGCGTGG GCTGGCCCTG GGGCATGAGG CTCACCTGCA GCGGCTCCGG 
GAAGCCCCGC CATCCCCGCT GACCTCCTTA GCGCTCCTAG AGGAATACGT GGACCAGCGG 
CTGCAGCGAC TCTGGGGGAG CCTGCTGGAT GGCTTTGAGC AGAAGCTGCA AGGTGTCCAG 
AGCGAGTGTG ACCTTCGGGT GCAAGAGGTG CGGCAGCAAT GTGAGGAGGG CCAGGCAGCC 
AGCCAGCGCT TGCATCAGAG CCTCGATGGC CGAGAGCTTG CCTTGCGTCG GGAGCTCTCC 
CAGCTGGGCA CTCAGCTGCA GGGCTTGACT CTGACTGGTG GGGGCACCTG CTGCAGCCAA 
CTGGCTTTGA TCAGTGCCCG GGTGGACAGC CTTGAGAGGA ACCTGCAGGC TGTCACTGAG 
ACCCAAGGTG GCCCGGGCAC CCTGGCCGCG GATGAGCTTG CGCGCCTCTC TGCAGCTATG 
CTTCAGGGAG GTGTGGACGG GCTGCTTGAA GGCCTTGAGA CTATCAATGG GACAGAGAAT 
GGAGCAAGGG GCTGCTGTCT GAGAATGGAG GTGGGGGGCT GGGGTGTCGG TGGCTTCGGG 
TCCACGCTGG AACAGCGAGT GCAGAGCCTA GAGGAGCGGC TGGCAACTCT GACCGGAGAG 
CTAAGCCCCG AGAGTGCCAT ACCAGACAGA TCAGCGCGGC CGCTTGTTCA TTCAGAACTA 
GCAGTACTGG AACAAAGGCT GGTGTCCCTG GAGACCTCGT GCACCCCAAG TACCACCACA 
GCCATCCTGG ACAACCTCGT GGCAGAGGTG AAGGCCTGGC AGAGCCGGAG CGAGGCTCTC 
CTACACCAGG TGGCTCGTCA CACTGCCCTG CTCCAACAGC TCAATGGCAC GGTAGCTGAA 
GTCCAGGGGC AGCTGGCAGA AGGGACAGGC AGCTCACTGC AAGGGGAGAT CACTCTCTTG 
AAAGTCAACC TGAACTCAGT GAGCAAGTCA CTCACGGGCC TCAGCGACTC TGTCAGCCAG 
TACTCTGATG CCTTCTCGGC TGCCAACACA TCCCTGGATG AACGAGAACG AAGAGTGGAA 
GCCGAGGTCC ACACCATCCA GGAACAGATC AGCAGCCAGG GCTCACGGCT TCAGGCTGGC 
CACAGGCAGG TCCTGAACCT GCGAGGGGAG CTGGAGCAAC TCAAGGCTGG CATGGCCAAT 
GTGGCTAGAG GGTTGAGCCG CTGCCGGGAC ACAGCCCAGG AACTCCAGCA TACAGTGGGC 
CACTTTGACC AGAGGGTGGC CCAAGTGGAA GGAGCATGTG AGAGGTTGGG CCTGTTGGCA 
ACCCACCTGA ACAGTCTGCC TACTGAGCAG CTGAGGTCCA GGGAGGGCCT GTGGGGCCAC 
ATAGACAAGC TGAACCACAC ATTGGCCCAG CACACGCAGG ATATCGCCCG GCTCCGGGAT 
GACCTGTTGG ACTGCAGAGC TCAGCTGGCT GAGGTGAGGC CAGGGCGAGC TGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_878260.1
CDS	169..2445
Translation	MGRRLSVWLCTVAALLSGAQAKGTPLLARPAQPSASRYSLYTTGWRPRLRPGPHKSLCAYVVHRNVTCVLQEGAESYIKAEYRNCGWGPNCPSTVRYRTVFRPRYKIGYKTVTDLAWRCCPGLTGESCPEHLTDHGATPPHQEPEPQIPLGQLGPGPRPSPYSREAPRPRGRKGQGPFGERLEQRLSQAYGTLSGLVASHENPNRITGDSRAPVVPIGFGVIPEGLVAPEDRGRGPLIPPLSEILSKVTEVSNTLQTKVQLLDEVRGLALGHEAHLQRLREAPPSPLTSLALLEEYVDQRLQRLWGSLLDGFEQKLQGVQSECDLRVQEVRQQCEEGQAASQRLHQSLDGRELALRRELSQLGTQLQGLTLTGGGTCCSQLALISARVDSLERNLQAVTETQGGPGTLAADELARLSAAMLQGGVDGLLEGLETINGTENGARGCCLRMEVGGWGVGGFGSTLEQRVQSLEERLATLTGELSPESAIPDRSARPLVHSELAVLEQRLVSLETSCTPSTTTAILDNLVAEVKAWQSRSEALLHQVARHTALLQQLNGTVAEVQGQLAEGTGSSLQGEITLLKVNLNSVSKSLTGLSDSVSQYSDAFSAANTSLDERERRVEAEVHTIQEQISSQGSRLQAGHRQVLNLRGELEQLKAGMANVARGLSRCRDTAQELQHTVGHFDQRVAQVEGACERLGLLATHLNSLPTEQLRSREGLWGHIDKLNHTLAQHTQDIARLRDDLLDCRAQLAEVRPGRAD

Target ORF information:

RefSeq Version	NM_182840.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus elastin microfibril interfacer 3 (Emilin3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182840.2

ORF Insert Sequence:

ATGGGCCGCC GTCTGTCCGT GTGGCTGTGC ACCGTCGCGG CGCTGCTCTC GGGGGCGCAG 
GCCAAGGGCA CCCCTCTCCT CGCGCGGCCC GCACAGCCCA GTGCCTCCCG TTACAGCCTC 
TACACCACCG GATGGCGTCC GCGGCTGCGC CCGGGGCCAC ACAAGTCTCT CTGTGCCTAT 
GTGGTACACA GAAATGTGAC CTGCGTCCTA CAAGAAGGAG CAGAGAGCTA TATAAAGGCT 
GAGTACCGGA ACTGTGGGTG GGGACCCAAC TGCCCTTCAA CAGTCAGGTA CCGCACAGTG 
TTCAGACCCA GATACAAGAT TGGCTACAAG ACCGTGACAG ACCTGGCGTG GCGCTGCTGC 
CCTGGTCTTA CTGGGGAAAG CTGCCCTGAA CACCTCACAG ACCATGGAGC CACCCCACCC 
CACCAGGAGC CAGAGCCTCA GATTCCCTTG GGGCAGCTGG GCCCAGGTCC CAGGCCTTCT 
CCTTACAGCA GAGAGGCCCC GCGCCCCCGT GGAAGAAAAG GCCAAGGGCC GTTTGGTGAG 
CGGCTAGAAC AGCGCCTGTC GCAAGCATAT GGCACCCTGA GTGGATTGGT AGCCAGCCAC 
GAGAATCCCA ATAGGATCAC TGGTGACTCC AGGGCCCCTG TCGTACCTAT AGGCTTTGGG 
GTCATCCCCG AGGGTCTTGT GGCCCCAGAA GACAGAGGCA GAGGACCACT CATCCCTCCT 
CTGAGTGAGA TCCTGAGCAA GGTCACAGAG GTGAGCAACA CATTACAGAC CAAGGTGCAG 
CTGTTAGATG AGGTGCGTGG GCTGGCCCTG GGGCATGAGG CTCACCTGCA GCGGCTCCGG 
GAAGCCCCGC CATCCCCGCT GACCTCCTTA GCGCTCCTAG AGGAATACGT GGACCAGCGG 
CTGCAGCGAC TCTGGGGGAG CCTGCTGGAT GGCTTTGAGC AGAAGCTGCA AGGTGTCCAG 
AGCGAGTGTG ACCTTCGGGT GCAAGAGGTG CGGCAGCAAT GTGAGGAGGG CCAGGCAGCC 
AGCCAGCGCT TGCATCAGAG CCTCGATGGC CGAGAGCTTG CCTTGCGTCG GGAGCTCTCC 
CAGCTGGGCA CTCAGCTGCA GGGCTTGACT CTGACTGGTG GGGGCACCTG CTGCAGCCAA 
CTGGCTTTGA TCAGTGCCCG GGTGGACAGC CTTGAGAGGA ACCTGCAGGC TGTCACTGAG 
ACCCAAGGTG GCCCGGGCAC CCTGGCCGCG GATGAGCTTG CGCGCCTCTC TGCAGCTATG 
CTTCAGGGAG GTGTGGACGG GCTGCTTGAA GGCCTTGAGA CTATCAATGG GACAGAGAAT 
GGAGCAAGGG GCTGCTGTCT GAGAATGGAG GTGGGGGGCT GGGGTGTCGG TGGCTTCGGG 
TCCACGCTGG AACAGCGAGT GCAGAGCCTA GAGGAGCGGC TGGCAACTCT GACCGGAGAG 
CTAAGCCCCG AGAGTGCCAT ACCAGACAGA TCAGCGCGGC CGCTTGTTCA TTCAGAACTA 
GCAGTACTGG AACAAAGGCT GGTGTCCCTG GAGACCTCGT GCACCCCAAG TACCACCACA 
GCCATCCTGG ACAACCTCGT GGCAGAGGTG AAGGCCTGGC AGAGCCGGAG CGAGGCTCTC 
CTACACCAGG TGGCTCGTCA CACTGCCCTG CTCCAACAGC TCAATGGCAC GGTAGCTGAA 
GTCCAGGGGC AGCTGGCAGA AGGGACAGGC AGCTCACTGC AAGGGGAGAT CACTCTCTTG 
AAAGTCAACC TGAACTCAGT GAGCAAGTCA CTCACGGGCC TCAGCGACTC TGTCAGCCAG 
TACTCTGATG CCTTCTCGGC TGCCAACACA TCCCTGGATG AACGAGAACG AAGAGTGGAA 
GCCGAGGTCC ACACCATCCA GGAACAGATC AGCAGCCAGG GCTCACGGCT TCAGGCTGGC 
CACAGGCAGG TCCTGAACCT GCGAGGGGAG CTGGAGCAAC TCAAGGCTGG CATGGCCAAT 
GTGGCTAGAG GGTTGAGCCG CTGCCGGGAC ACAGCCCAGG AACTCCAGCA TACAGTGGGC 
CACTTTGACC AGAGGGTGGC CCAAGTGGAA GGAGCATGTG AGAGGTTGGG CCTGTTGGCA 
ACCCACCTGA ACAGTCTGCC TACTGAGCAG CTGAGGTCCA GGGAGGGCCT GTGGGGCCAC 
ATAGACAAGC TGAACCACAC ATTGGCCCAG CACACGCAGG ATATCGCCCG GCTCCGGGAT 
GACCTGTTGG ACTGCAGAGC TCAGCTGGCT GAGGTGAGGC CAGGGCGAGC TGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Altered feto-placental vascularization, feto-placental malperfusion and fetal growth restriction in mice with
Development (Cambridge, England)144(13)2469-2479(2017 07)
Lacko LA,Hurtado R,Hinds S,Poulos MG,Butler JM,Stuhlmann H

EMILIN3, an extracellular matrix molecule with restricted distribution in skin.
Experimental dermatology26(5)435-438(2017 May)
Corallo D,Schiavinato A,Bizzotto D,Milanetto M,Guljelmovic M,Keene DR,Sengle G,Braghetta P,Bonaldo P

Transcriptome of the inner circular smooth muscle of the developing mouse intestine: Evidence for regulation of visceral smooth muscle genes by the hedgehog target gene, cJun.
Developmental dynamics : an official publication of the American Association of Anatomists245(5)614-26(2016 May)
Gurdziel K,Vogt KR,Walton KD,Schneider GK,Gumucio DL

EMILIN-3, peculiar member of elastin microfibril interface-located protein (EMILIN) family, has distinct expression pattern, forms oligomeric assemblies, and serves as transforming growth factor ? (TGF-?) antagonist.
The Journal of biological chemistry287(14)11498-515(2012 Mar)
Schiavinato A,Becker AK,Zanetti M,Corallo D,Milanetto M,Bizzotto D,Bressan G,Guljelmovic M,Paulsson M,Wagener R,Braghetta P,Bonaldo P

Genomewide discovery and classification of candidate ovarian fertility genes in the mouse.
Genetics177(1)179-94(2007 Sep)
Gallardo TD,John GB,Shirley L,Contreras CM,Akbay EA,Haynie JM,Ward SE,Shidler MJ,Castrillon DH