EMILIN3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EMILIN3
Entrez Gene ID	90187
Full Name	elastin microfibril interfacer 3
Synonyms	C20orf130,EMILIN5,dJ620E11.4
General protein information	Preferred Names elastin microfibril interfacer 3 Names EMILIN-3 EMILIN-5 elastin microfibril interface-located protein 3 elastin microfibril interface-located protein 5 elastin microfibril interfacer 5
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20q12
Disorder MIM:	608929

mRNA and Protein(s)

mRNA	Protein	Name
NM_052846.1	NP_443078.1	EMILIN-3 precursor
NM_052846.2	NP_443078.1	EMILIN-3 precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	emilin3a	XP_699102.3
Rattus norvegicus (Norway rat)	Emilin3	NP_001103371.1
	EMILIN3	XP_001086225.1
Bos taurus (cattle)	EMILIN3	XP_003586866.2
Mus musculus (house mouse)	Emilin3	NP_878260.1
Gallus gallus (chicken)	EMILIN3	XP_425694.4
Danio rerio (zebrafish)	LOC101882042	XP_005161982.1
Xenopus tropicalis (tropical clawed frog)	emilin3	XP_002932911.2
Homo sapiens (human)	EMILIN3	NP_443078.1
Pan troglodytes (chimpanzee)	EMILIN3	XP_514653.2
Canis lupus familiaris (dog)	EMILIN3	XP_543001.2

Gene Ontology
Bibliography

Related articles in PubMed

Extracellular matrix signatures of human primary metastatic colon cancers and their metastases to liver.
Naba A, Clauser KR, Whittaker CA, Carr SA, Tanabe KK, Hynes RO
BMC cancer14518(2014 Jul)

EMILIN-3, peculiar member of elastin microfibril interface-located protein (EMILIN) family, has distinct expression pattern, forms oligomeric assemblies, and serves as transforming growth factor ? (TGF-?) antagonist.
Schiavinato A, Becker AK, Zanetti M, Corallo D, Milanetto M, Bizzotto D, Bressan G, Guljelmovic M, Paulsson M, Wagener R, Braghetta P, Bonaldo P
The Journal of biological chemistry287(14)11498-515(2012 Mar)

Proteomics analysis of cardiac extracellular matrix remodeling in a porcine model of ischemia/reperfusion injury.
Barallobre-Barreiro J, Didangelos A, Schoendube FA, Drozdov I, Yin X, Fern?ndez-Caggiano M, Willeit P, Puntmann VO, Aldama-L?pez G, Shah AM, Dom?nech N, Mayr M
Circulation125(6)789-802(2012 Feb)

Preparation and characterization of a novel monoclonal antibody specific to human EMILIN-5 protein.
Cao J
Hybridoma (2005)26(6)417-21(2007 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

characterization of the expression and biochemical properties of EMILIN-3
Title: EMILIN-3, peculiar member of elastin microfibril interface-located protein (EMILIN) family, has distinct expression pattern, forms oligomeric assemblies, and serves as transforming growth factor ? (TGF-?) antagonist.

Might play important roles in carcinogenesis of esophageal cancer.
Title: Preparation and characterization of a novel monoclonal antibody specific to human EMILIN-5 protein.

EMILIN-5 gene plays an important role in skeletal development(EMILIN-5)
Title: Molecular cloning and characterization of a novel gene, EMILIN-5, and its possible involvement in skeletal development.

The following EMILIN3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EMILIN3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05524	NM_052846.2 Latest version!	Homo sapiens elastin microfibril interfacer 3 (EMILIN3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$384.50-$538.30 $769.00
View Old Accession Versions >>
OHu05524	NM_052846.1	Homo sapiens elastin microfibril interfacer 3 (EMILIN3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$384.50-$538.30 $769.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EMILIN3 ( NM_052846.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_443078.1 Homo sapiens elastin microfibril interfacer 3 (EMILIN3), mRNA.

Price & Availability↑

CloneID	OHu05524
Clone ID Related Accession (Same CDS sequence)	NM_052846.1 , NM_052846.2
Accession Version	NM_052846.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	EMILIN-3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB089149.1. On Mar 22, 2004 this sequence version replaced XM_029741.4. ##Evidence-Data-START## Transcript exon combination :: AB089149.1, BC101043.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END##

ATGGGCCGCC GCCGCCTGCT CGTCTGGCTG TGCGCCGTCG CGGCGCTGCT CTCGGGGGCG 
CAGGCCAGGG GCACCCCGCT CCTGGCGCGG CCTGCGCCGC CCGGTGCCTC CCGCTACAGT 
CTCTACACGA CGGGATGGCG CCCGCGGCTG CGCCCGGGGC CGCACAAGGC CCTCTGTGCC 
TATGTGGTGC ACAGGAATGT GACCTGCATC CTACAGGAGG GAGCGGAGAG CTACGTAAAG 
GCTGAATACC GGCAGTGTAG ATGGGGGCCC AAGTGCCCCG GGACAGTCAC GTACCGCACA 
GTACTCAGAC CCAAATACAA GGTTGGCTAC AAGACAGTGA CAGACCTCGC CTGGCGTTGC 
TGTCCCGGCT TCACTGGGAA ACGCTGCCCT GAGCACCTCA CGGACCATGG GGCTGCCTCA 
CCCCAGCTGG AGCCTGAGCC TCAGATTCCT TCAGGGCAGC TGGACCCAGG CCCCAGGCCC 
CCTTCCTACA GCAGAGCAGC CCCCAGCCCT CATGGAAGGA AAGGCCCAGG GCTGTTTGGT 
GAGCGGCTGG AACGCCTGGA GGGTGATGTC CAGCGCCTGG CTCAAACATA TGGTACCCTC 
AGTGGCCTGG TGGCTAGCCA CGAGGATCCC AACAGGATGA CTGGTGGCCC CAGGGCTCCT 
GCTGTCCCTG TGGGCTTTGG GGTCATCCCT GAGGGGCTTG TGGGCCCAGG AGACAGAGCC 
AGAGGGCCAC TAACACCTCC CTTAGACGAG ATCCTAAGCA AGGTGACAGA GGTGAGCAAC 
ACTCTTCAGA CCAAGGTGCA GCTTCTAGAC AAGGTGCATG GGCTGGCACT TGGCCATGAG 
GCCCACCTGC AGCGGCTGCG GGAAGCCCCA CCATCCCCGC TCACCTCCCT GGCCCTGCTG 
GAGGAGTACG TGGACCGACG GCTGCACCGA CTCTGGGGGA GCCTGCTGGA TGGCTTTGAG 
CAGAAGCTGC AAGGCGTCCA GAGTGAGTGT GACCTGCGGG TGCAGGAGGT ACGGCGGCAA 
TGTGAGGAGG GTCAGGCCGC CAGCCGGAGG CTGCACCAGA GCCTTGATGG CCGGGAGCTG 
GCCCTGCGCC AGGAGCTGTC ACAGCTGGGC AGCCAGCTGC AGGGCCTGAG CGTGTCTGGC 
AGGGGCAGCT GCTGTGGGCA ACTAGCCTTG ATCAATGCCC GTATGGATGG CCTTGAGAGG 
GCCCTGCAGG CAGTCACCGA GACCCAAAGG GGCCCCGGTG CCCCGGCCGG GGATGAGCTT 
ACGAGGCTCT CTGCTGCCAT GCTCGAGGGA GGTGTGGACG GGCTGCTTGA GGGTCTGGAG 
ACGCTCAATG GGACAGAGGG TGGAGCAAGG GGATGCTGTC TGAGGTTGGA CATGGGGGGG 
TGGGGAGTGG GCGGCTTTGG GACCATGCTG GAAGAGCGCG TGCAGAGCCT CGAGGAGCGC 
CTAGCAACAT TGGCTGGGGA GCTAAGCCAT GACAGCGCCT CTCCGGGCAG GTCAGCTCGG 
CCCCTTGTAC AGACAGAGCT GGCTGTGCTA GAGCAACGGT TGGTCTCACT GGAGACTTCG 
TGCACCCCGA GCACCACCTC AGCCATCCTG GACAGCCTCG TGGCAGAGGT GAAGGCCTGG 
CAGAGCCGGA GCGAGGCCCT CCTACGCCAG GTGGCCAGCC ACGCAGCACT GCTCCAGCAG 
CTCAATGGCA CTGTGGCCGA GGTCCAGGGA CAGCTGGCAG AAGGGACGGG CAGCTCACTT 
CAAGGCGAGA TCACTCTGCT CAAGGTCAAT CTGAACTCAG TGAGCAAGTC GCTCACAGGC 
CTCAGTGACT CTGTCAGCCA GTACTCTGAT GCCTTCTTGG CTGCCAACAC GTCCCTGGAT 
GAGCGGGAAC GCAAGGTGGA AGCCGAAGTC CAGGCCATCC AGGAGCAGGT CAGCAGCCAA 
GGCTCCAGGC TTCAGGCTGG CCACAGGCAG GTCCTGAACC TGCGTGGGGA GCTGGAGCAA 
CTCAAGGCTG GTGTGGCCAA GGTGGCCAGT GGGCTGAGCC GCTGCCAGGA CACAGCCCAG 
AAACTTCAGC ACACAGTGGG ACACTTTGAC CAGCGGGTGG CACAAGTGGA GGGTGCGTGC 
AGGAGGCTGG GCCTGCTGGC CGCGGGCCTG GACAGCTTGC CCACTGAGCC ACTGAGGCCC 
AGAGAGGGCC TGTGGAGCCA TGTGGACCAG CTGAATCGTA CGCTGGCCCA GCACACGCAG 
GACATTGCCC GCCTCCGGGA TGACCTACTG GACTGCCAGG CCCAGCTGGC TGAGCAGGTG 
CGGCCAGGGC AAGCCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_443078.1
CDS	225..2525
Translation	MGRRRLLVWLCAVAALLSGAQARGTPLLARPAPPGASRYSLYTTGWRPRLRPGPHKALCAYVVHRNVTCILQEGAESYVKAEYRQCRWGPKCPGTVTYRTVLRPKYKVGYKTVTDLAWRCCPGFTGKRCPEHLTDHGAASPQLEPEPQIPSGQLDPGPRPPSYSRAAPSPHGRKGPGLFGERLERLEGDVQRLAQTYGTLSGLVASHEDPNRMTGGPRAPAVPVGFGVIPEGLVGPGDRARGPLTPPLDEILSKVTEVSNTLQTKVQLLDKVHGLALGHEAHLQRLREAPPSPLTSLALLEEYVDRRLHRLWGSLLDGFEQKLQGVQSECDLRVQEVRRQCEEGQAASRRLHQSLDGRELALRQELSQLGSQLQGLSVSGRGSCCGQLALINARMDGLERALQAVTETQRGPGAPAGDELTRLSAAMLEGGVDGLLEGLETLNGTEGGARGCCLRLDMGGWGVGGFGTMLEERVQSLEERLATLAGELSHDSASPGRSARPLVQTELAVLEQRLVSLETSCTPSTTSAILDSLVAEVKAWQSRSEALLRQVASHAALLQQLNGTVAEVQGQLAEGTGSSLQGEITLLKVNLNSVSKSLTGLSDSVSQYSDAFLAANTSLDERERKVEAEVQAIQEQVSSQGSRLQAGHRQVLNLRGELEQLKAGVAKVASGLSRCQDTAQKLQHTVGHFDQRVAQVEGACRRLGLLAAGLDSLPTEPLRPREGLWSHVDQLNRTLAQHTQDIARLRDDLLDCQAQLAEQVRPGQAN

Target ORF information:

RefSeq Version	NM_052846.1
Organism	Homo sapiens(human)
Definition	Homo sapiens elastin microfibril interfacer 3 (EMILIN3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_052846.1

ORF Insert Sequence:

ATGGGCCGCC GCCGCCTGCT CGTCTGGCTG TGCGCCGTCG CGGCGCTGCT CTCGGGGGCG 
CAGGCCAGGG GCACCCCGCT CCTGGCGCGG CCTGCGCCGC CCGGTGCCTC CCGCTACAGT 
CTCTACACGA CGGGATGGCG CCCGCGGCTG CGCCCGGGGC CGCACAAGGC CCTCTGTGCC 
TATGTGGTGC ACAGGAATGT GACCTGCATC CTACAGGAGG GAGCGGAGAG CTACGTAAAG 
GCTGAATACC GGCAGTGTAG ATGGGGGCCC AAGTGCCCCG GGACAGTCAC GTACCGCACA 
GTACTCAGAC CCAAATACAA GGTTGGCTAC AAGACAGTGA CAGACCTCGC CTGGCGTTGC 
TGTCCCGGCT TCACTGGGAA ACGCTGCCCT GAGCACCTCA CGGACCATGG GGCTGCCTCA 
CCCCAGCTGG AGCCTGAGCC TCAGATTCCT TCAGGGCAGC TGGACCCAGG CCCCAGGCCC 
CCTTCCTACA GCAGAGCAGC CCCCAGCCCT CATGGAAGGA AAGGCCCAGG GCTGTTTGGT 
GAGCGGCTGG AACGCCTGGA GGGTGATGTC CAGCGCCTGG CTCAAACATA TGGTACCCTC 
AGTGGCCTGG TGGCTAGCCA CGAGGATCCC AACAGGATGA CTGGTGGCCC CAGGGCTCCT 
GCTGTCCCTG TGGGCTTTGG GGTCATCCCT GAGGGGCTTG TGGGCCCAGG AGACAGAGCC 
AGAGGGCCAC TAACACCTCC CTTAGACGAG ATCCTAAGCA AGGTGACAGA GGTGAGCAAC 
ACTCTTCAGA CCAAGGTGCA GCTTCTAGAC AAGGTGCATG GGCTGGCACT TGGCCATGAG 
GCCCACCTGC AGCGGCTGCG GGAAGCCCCA CCATCCCCGC TCACCTCCCT GGCCCTGCTG 
GAGGAGTACG TGGACCGACG GCTGCACCGA CTCTGGGGGA GCCTGCTGGA TGGCTTTGAG 
CAGAAGCTGC AAGGCGTCCA GAGTGAGTGT GACCTGCGGG TGCAGGAGGT ACGGCGGCAA 
TGTGAGGAGG GTCAGGCCGC CAGCCGGAGG CTGCACCAGA GCCTTGATGG CCGGGAGCTG 
GCCCTGCGCC AGGAGCTGTC ACAGCTGGGC AGCCAGCTGC AGGGCCTGAG CGTGTCTGGC 
AGGGGCAGCT GCTGTGGGCA ACTAGCCTTG ATCAATGCCC GTATGGATGG CCTTGAGAGG 
GCCCTGCAGG CAGTCACCGA GACCCAAAGG GGCCCCGGTG CCCCGGCCGG GGATGAGCTT 
ACGAGGCTCT CTGCTGCCAT GCTCGAGGGA GGTGTGGACG GGCTGCTTGA GGGTCTGGAG 
ACGCTCAATG GGACAGAGGG TGGAGCAAGG GGATGCTGTC TGAGGTTGGA CATGGGGGGG 
TGGGGAGTGG GCGGCTTTGG GACCATGCTG GAAGAGCGCG TGCAGAGCCT CGAGGAGCGC 
CTAGCAACAT TGGCTGGGGA GCTAAGCCAT GACAGCGCCT CTCCGGGCAG GTCAGCTCGG 
CCCCTTGTAC AGACAGAGCT GGCTGTGCTA GAGCAACGGT TGGTCTCACT GGAGACTTCG 
TGCACCCCGA GCACCACCTC AGCCATCCTG GACAGCCTCG TGGCAGAGGT GAAGGCCTGG 
CAGAGCCGGA GCGAGGCCCT CCTACGCCAG GTGGCCAGCC ACGCAGCACT GCTCCAGCAG 
CTCAATGGCA CTGTGGCCGA GGTCCAGGGA CAGCTGGCAG AAGGGACGGG CAGCTCACTT 
CAAGGCGAGA TCACTCTGCT CAAGGTCAAT CTGAACTCAG TGAGCAAGTC GCTCACAGGC 
CTCAGTGACT CTGTCAGCCA GTACTCTGAT GCCTTCTTGG CTGCCAACAC GTCCCTGGAT 
GAGCGGGAAC GCAAGGTGGA AGCCGAAGTC CAGGCCATCC AGGAGCAGGT CAGCAGCCAA 
GGCTCCAGGC TTCAGGCTGG CCACAGGCAG GTCCTGAACC TGCGTGGGGA GCTGGAGCAA 
CTCAAGGCTG GTGTGGCCAA GGTGGCCAGT GGGCTGAGCC GCTGCCAGGA CACAGCCCAG 
AAACTTCAGC ACACAGTGGG ACACTTTGAC CAGCGGGTGG CACAAGTGGA GGGTGCGTGC 
AGGAGGCTGG GCCTGCTGGC CGCGGGCCTG GACAGCTTGC CCACTGAGCC ACTGAGGCCC 
AGAGAGGGCC TGTGGAGCCA TGTGGACCAG CTGAATCGTA CGCTGGCCCA GCACACGCAG 
GACATTGCCC GCCTCCGGGA TGACCTACTG GACTGCCAGG CCCAGCTGGC TGAGCAGGTG 
CGGCCAGGGC AAGCCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMILIN3 ( NM_052846.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_443078.1 Homo sapiens elastin microfibril interfacer 3 (EMILIN3), mRNA.

Price & Availability↑

CloneID	OHu05524
Clone ID Related Accession (Same CDS sequence)	NM_052846.1 , NM_052846.2
Accession Version	NM_052846.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	EMILIN-3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB089149.1 and AL031667.21. On Nov 22, 2018 this sequence version replaced NM_052846.1. ##Evidence-Data-START## Transcript exon combination :: AB089149.1, BC101043.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000332312.4/ ENSP00000332806.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCCGCC GCCGCCTGCT CGTCTGGCTG TGCGCCGTCG CGGCGCTGCT CTCGGGGGCG 
CAGGCCAGGG GCACCCCGCT CCTGGCGCGG CCTGCGCCGC CCGGTGCCTC CCGCTACAGT 
CTCTACACGA CGGGATGGCG CCCGCGGCTG CGCCCGGGGC CGCACAAGGC CCTCTGTGCC 
TATGTGGTGC ACAGGAATGT GACCTGCATC CTACAGGAGG GAGCGGAGAG CTACGTAAAG 
GCTGAATACC GGCAGTGTAG ATGGGGGCCC AAGTGCCCCG GGACAGTCAC GTACCGCACA 
GTACTCAGAC CCAAATACAA GGTTGGCTAC AAGACAGTGA CAGACCTCGC CTGGCGTTGC 
TGTCCCGGCT TCACTGGGAA ACGCTGCCCT GAGCACCTCA CGGACCATGG GGCTGCCTCA 
CCCCAGCTGG AGCCTGAGCC TCAGATTCCT TCAGGGCAGC TGGACCCAGG CCCCAGGCCC 
CCTTCCTACA GCAGAGCAGC CCCCAGCCCT CATGGAAGGA AAGGCCCAGG GCTGTTTGGT 
GAGCGGCTGG AACGCCTGGA GGGTGATGTC CAGCGCCTGG CTCAAACATA TGGTACCCTC 
AGTGGCCTGG TGGCTAGCCA CGAGGATCCC AACAGGATGA CTGGTGGCCC CAGGGCTCCT 
GCTGTCCCTG TGGGCTTTGG GGTCATCCCT GAGGGGCTTG TGGGCCCAGG AGACAGAGCC 
AGAGGGCCAC TAACACCTCC CTTAGACGAG ATCCTAAGCA AGGTGACAGA GGTGAGCAAC 
ACTCTTCAGA CCAAGGTGCA GCTTCTAGAC AAGGTGCATG GGCTGGCACT TGGCCATGAG 
GCCCACCTGC AGCGGCTGCG GGAAGCCCCA CCATCCCCGC TCACCTCCCT GGCCCTGCTG 
GAGGAGTACG TGGACCGACG GCTGCACCGA CTCTGGGGGA GCCTGCTGGA TGGCTTTGAG 
CAGAAGCTGC AAGGCGTCCA GAGTGAGTGT GACCTGCGGG TGCAGGAGGT ACGGCGGCAA 
TGTGAGGAGG GTCAGGCCGC CAGCCGGAGG CTGCACCAGA GCCTTGATGG CCGGGAGCTG 
GCCCTGCGCC AGGAGCTGTC ACAGCTGGGC AGCCAGCTGC AGGGCCTGAG CGTGTCTGGC 
AGGGGCAGCT GCTGTGGGCA ACTAGCCTTG ATCAATGCCC GTATGGATGG CCTTGAGAGG 
GCCCTGCAGG CAGTCACCGA GACCCAAAGG GGCCCCGGTG CCCCGGCCGG GGATGAGCTT 
ACGAGGCTCT CTGCTGCCAT GCTCGAGGGA GGTGTGGACG GGCTGCTTGA GGGTCTGGAG 
ACGCTCAATG GGACAGAGGG TGGAGCAAGG GGATGCTGTC TGAGGTTGGA CATGGGGGGG 
TGGGGAGTGG GCGGCTTTGG GACCATGCTG GAAGAGCGCG TGCAGAGCCT CGAGGAGCGC 
CTAGCAACAT TGGCTGGGGA GCTAAGCCAT GACAGCGCCT CTCCGGGCAG GTCAGCTCGG 
CCCCTTGTAC AGACAGAGCT GGCTGTGCTA GAGCAACGGT TGGTCTCACT GGAGACTTCG 
TGCACCCCGA GCACCACCTC AGCCATCCTG GACAGCCTCG TGGCAGAGGT GAAGGCCTGG 
CAGAGCCGGA GCGAGGCCCT CCTACGCCAG GTGGCCAGCC ACGCAGCACT GCTCCAGCAG 
CTCAATGGCA CTGTGGCCGA GGTCCAGGGA CAGCTGGCAG AAGGGACGGG CAGCTCACTT 
CAAGGCGAGA TCACTCTGCT CAAGGTCAAT CTGAACTCAG TGAGCAAGTC GCTCACAGGC 
CTCAGTGACT CTGTCAGCCA GTACTCTGAT GCCTTCTTGG CTGCCAACAC GTCCCTGGAT 
GAGCGGGAAC GCAAGGTGGA AGCCGAAGTC CAGGCCATCC AGGAGCAGGT CAGCAGCCAA 
GGCTCCAGGC TTCAGGCTGG CCACAGGCAG GTCCTGAACC TGCGTGGGGA GCTGGAGCAA 
CTCAAGGCTG GTGTGGCCAA GGTGGCCAGT GGGCTGAGCC GCTGCCAGGA CACAGCCCAG 
AAACTTCAGC ACACAGTGGG ACACTTTGAC CAGCGGGTGG CACAAGTGGA GGGTGCGTGC 
AGGAGGCTGG GCCTGCTGGC CGCGGGCCTG GACAGCTTGC CCACTGAGCC ACTGAGGCCC 
AGAGAGGGCC TGTGGAGCCA TGTGGACCAG CTGAATCGTA CGCTGGCCCA GCACACGCAG 
GACATTGCCC GCCTCCGGGA TGACCTACTG GACTGCCAGG CCCAGCTGGC TGAGCAGGTG 
CGGCCAGGGC AAGCCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_443078.1
CDS	185..2485
Translation	MGRRRLLVWLCAVAALLSGAQARGTPLLARPAPPGASRYSLYTTGWRPRLRPGPHKALCAYVVHRNVTCILQEGAESYVKAEYRQCRWGPKCPGTVTYRTVLRPKYKVGYKTVTDLAWRCCPGFTGKRCPEHLTDHGAASPQLEPEPQIPSGQLDPGPRPPSYSRAAPSPHGRKGPGLFGERLERLEGDVQRLAQTYGTLSGLVASHEDPNRMTGGPRAPAVPVGFGVIPEGLVGPGDRARGPLTPPLDEILSKVTEVSNTLQTKVQLLDKVHGLALGHEAHLQRLREAPPSPLTSLALLEEYVDRRLHRLWGSLLDGFEQKLQGVQSECDLRVQEVRRQCEEGQAASRRLHQSLDGRELALRQELSQLGSQLQGLSVSGRGSCCGQLALINARMDGLERALQAVTETQRGPGAPAGDELTRLSAAMLEGGVDGLLEGLETLNGTEGGARGCCLRLDMGGWGVGGFGTMLEERVQSLEERLATLAGELSHDSASPGRSARPLVQTELAVLEQRLVSLETSCTPSTTSAILDSLVAEVKAWQSRSEALLRQVASHAALLQQLNGTVAEVQGQLAEGTGSSLQGEITLLKVNLNSVSKSLTGLSDSVSQYSDAFLAANTSLDERERKVEAEVQAIQEQVSSQGSRLQAGHRQVLNLRGELEQLKAGVAKVASGLSRCQDTAQKLQHTVGHFDQRVAQVEGACRRLGLLAAGLDSLPTEPLRPREGLWSHVDQLNRTLAQHTQDIARLRDDLLDCQAQLAEQVRPGQAN

Target ORF information:

RefSeq Version	NM_052846.2
Organism	Homo sapiens(human)
Definition	Homo sapiens elastin microfibril interfacer 3 (EMILIN3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_052846.2

ORF Insert Sequence:

ATGGGCCGCC GCCGCCTGCT CGTCTGGCTG TGCGCCGTCG CGGCGCTGCT CTCGGGGGCG 
CAGGCCAGGG GCACCCCGCT CCTGGCGCGG CCTGCGCCGC CCGGTGCCTC CCGCTACAGT 
CTCTACACGA CGGGATGGCG CCCGCGGCTG CGCCCGGGGC CGCACAAGGC CCTCTGTGCC 
TATGTGGTGC ACAGGAATGT GACCTGCATC CTACAGGAGG GAGCGGAGAG CTACGTAAAG 
GCTGAATACC GGCAGTGTAG ATGGGGGCCC AAGTGCCCCG GGACAGTCAC GTACCGCACA 
GTACTCAGAC CCAAATACAA GGTTGGCTAC AAGACAGTGA CAGACCTCGC CTGGCGTTGC 
TGTCCCGGCT TCACTGGGAA ACGCTGCCCT GAGCACCTCA CGGACCATGG GGCTGCCTCA 
CCCCAGCTGG AGCCTGAGCC TCAGATTCCT TCAGGGCAGC TGGACCCAGG CCCCAGGCCC 
CCTTCCTACA GCAGAGCAGC CCCCAGCCCT CATGGAAGGA AAGGCCCAGG GCTGTTTGGT 
GAGCGGCTGG AACGCCTGGA GGGTGATGTC CAGCGCCTGG CTCAAACATA TGGTACCCTC 
AGTGGCCTGG TGGCTAGCCA CGAGGATCCC AACAGGATGA CTGGTGGCCC CAGGGCTCCT 
GCTGTCCCTG TGGGCTTTGG GGTCATCCCT GAGGGGCTTG TGGGCCCAGG AGACAGAGCC 
AGAGGGCCAC TAACACCTCC CTTAGACGAG ATCCTAAGCA AGGTGACAGA GGTGAGCAAC 
ACTCTTCAGA CCAAGGTGCA GCTTCTAGAC AAGGTGCATG GGCTGGCACT TGGCCATGAG 
GCCCACCTGC AGCGGCTGCG GGAAGCCCCA CCATCCCCGC TCACCTCCCT GGCCCTGCTG 
GAGGAGTACG TGGACCGACG GCTGCACCGA CTCTGGGGGA GCCTGCTGGA TGGCTTTGAG 
CAGAAGCTGC AAGGCGTCCA GAGTGAGTGT GACCTGCGGG TGCAGGAGGT ACGGCGGCAA 
TGTGAGGAGG GTCAGGCCGC CAGCCGGAGG CTGCACCAGA GCCTTGATGG CCGGGAGCTG 
GCCCTGCGCC AGGAGCTGTC ACAGCTGGGC AGCCAGCTGC AGGGCCTGAG CGTGTCTGGC 
AGGGGCAGCT GCTGTGGGCA ACTAGCCTTG ATCAATGCCC GTATGGATGG CCTTGAGAGG 
GCCCTGCAGG CAGTCACCGA GACCCAAAGG GGCCCCGGTG CCCCGGCCGG GGATGAGCTT 
ACGAGGCTCT CTGCTGCCAT GCTCGAGGGA GGTGTGGACG GGCTGCTTGA GGGTCTGGAG 
ACGCTCAATG GGACAGAGGG TGGAGCAAGG GGATGCTGTC TGAGGTTGGA CATGGGGGGG 
TGGGGAGTGG GCGGCTTTGG GACCATGCTG GAAGAGCGCG TGCAGAGCCT CGAGGAGCGC 
CTAGCAACAT TGGCTGGGGA GCTAAGCCAT GACAGCGCCT CTCCGGGCAG GTCAGCTCGG 
CCCCTTGTAC AGACAGAGCT GGCTGTGCTA GAGCAACGGT TGGTCTCACT GGAGACTTCG 
TGCACCCCGA GCACCACCTC AGCCATCCTG GACAGCCTCG TGGCAGAGGT GAAGGCCTGG 
CAGAGCCGGA GCGAGGCCCT CCTACGCCAG GTGGCCAGCC ACGCAGCACT GCTCCAGCAG 
CTCAATGGCA CTGTGGCCGA GGTCCAGGGA CAGCTGGCAG AAGGGACGGG CAGCTCACTT 
CAAGGCGAGA TCACTCTGCT CAAGGTCAAT CTGAACTCAG TGAGCAAGTC GCTCACAGGC 
CTCAGTGACT CTGTCAGCCA GTACTCTGAT GCCTTCTTGG CTGCCAACAC GTCCCTGGAT 
GAGCGGGAAC GCAAGGTGGA AGCCGAAGTC CAGGCCATCC AGGAGCAGGT CAGCAGCCAA 
GGCTCCAGGC TTCAGGCTGG CCACAGGCAG GTCCTGAACC TGCGTGGGGA GCTGGAGCAA 
CTCAAGGCTG GTGTGGCCAA GGTGGCCAGT GGGCTGAGCC GCTGCCAGGA CACAGCCCAG 
AAACTTCAGC ACACAGTGGG ACACTTTGAC CAGCGGGTGG CACAAGTGGA GGGTGCGTGC 
AGGAGGCTGG GCCTGCTGGC CGCGGGCCTG GACAGCTTGC CCACTGAGCC ACTGAGGCCC 
AGAGAGGGCC TGTGGAGCCA TGTGGACCAG CTGAATCGTA CGCTGGCCCA GCACACGCAG 
GACATTGCCC GCCTCCGGGA TGACCTACTG GACTGCCAGG CCCAGCTGGC TGAGCAGGTG 
CGGCCAGGGC AAGCCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Extracellular matrix signatures of human primary metastatic colon cancers and their metastases to liver.
BMC cancer14518(2014 Jul)
Naba A,Clauser KR,Whittaker CA,Carr SA,Tanabe KK,Hynes RO

EMILIN-3, peculiar member of elastin microfibril interface-located protein (EMILIN) family, has distinct expression pattern, forms oligomeric assemblies, and serves as transforming growth factor ? (TGF-?) antagonist.
The Journal of biological chemistry287(14)11498-515(2012 Mar)
Schiavinato A,Becker AK,Zanetti M,Corallo D,Milanetto M,Bizzotto D,Bressan G,Guljelmovic M,Paulsson M,Wagener R,Braghetta P,Bonaldo P

Proteomics analysis of cardiac extracellular matrix remodeling in a porcine model of ischemia/reperfusion injury.
Circulation125(6)789-802(2012 Feb)
Barallobre-Barreiro J,Didangelos A,Schoendube FA,Drozdov I,Yin X,Fern?ndez-Caggiano M,Willeit P,Puntmann VO,Aldama-L?pez G,Shah AM,Dom?nech N,Mayr M

Preparation and characterization of a novel monoclonal antibody specific to human EMILIN-5 protein.
Hybridoma (2005)26(6)417-21(2007 Dec)
Cao J

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EMILIN3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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