B9d2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	B9d2
Entrez Gene ID	232987
Full Name	B9 protein domain 2
Synonyms	stumpy
General protein information	Preferred Names B9 protein domain 2 Names B9 domain-containing protein 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 A3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006539854.3	XP_006539917.1	B9 domain-containing protein 2 isoform X1
NM_172148.2	NP_742160.1	B9 domain-containing protein 2
XM_006539854.2	XP_006539917.1	B9 domain-containing protein 2 isoform X1
NM_172148.1	NP_742160.1	B9 domain-containing protein 2
NM_001362154.1	NP_001349083.1	B9 domain-containing protein 2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	B9d2	NP_001100964.1
Bos taurus (cattle)	B9D2	NP_001035683.1
Mus musculus (house mouse)	B9d2	NP_742160.1
Drosophila melanogaster (fruit fly)	CG42730	NP_001188704.1
Caenorhabditis elegans (roundworm)	mksr-2	NP_500186.1
	B9D2	XP_001101041.1
Canis lupus familiaris (dog)	B9D2	XP_533658.1
Homo sapiens (human)	B9D2	NP_085055.2
Pan troglodytes (chimpanzee)	B9D2	XP_001140469.1
Danio rerio (zebrafish)	b9d2	NP_001002394.1
Xenopus tropicalis (tropical clawed frog)	b9d2	XP_002939434.1

Gene Ontology
Bibliography

Related articles in PubMed

The GARP/Latent TGF-?1 complex on Treg cells modulates the induction of peripherally derived Treg cells during oral tolerance.
Edwards JP, Hand TW, Morais da Fonseca D, Glass DD, Belkaid Y, Shevach EM
European journal of immunology46(6)1480-9(2016 06)

Generation of Th17 cells in response to intranasal infection requires TGF-?1 from dendritic cells and IL-6 from CD301b+ dendritic cells.
Linehan JL, Dileepan T, Kashem SW, Kaplan DH, Cleary P, Jenkins MK
Proceedings of the National Academy of Sciences of the United States of America112(41)12782-7(2015 Oct)

Release of active TGF-?1 from the latent TGF-?1/GARP complex on T regulatory cells is mediated by integrin ?8.
Edwards JP, Thornton AM, Shevach EM
Journal of immunology (Baltimore, Md. : 1950)193(6)2843-9(2014 Sep)

Macrophage-specific deletion of transforming growth factor-?1 does not prevent renal fibrosis after severe ischemia-reperfusion or obstructive injury.
Huen SC, Moeckel GW, Cantley LG
American journal of physiology. Renal physiology305(4)F477-84(2013 Aug)

Regulation of the expression of GARP/latent TGF-?1 complexes on mouse T cells and their role in regulatory T cell and Th17 differentiation.
Edwards JP, Fujii H, Zhou AX, Creemers J, Unutmaz D, Shevach EM
Journal of immunology (Baltimore, Md. : 1950)190(11)5506-15(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following B9d2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the B9d2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13079	NM_172148.2 Latest version!	Mus musculus B9 protein domain 2 (B9d2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu13079	NM_001362154.1 Latest version!	Mus musculus B9 protein domain 2 (B9d2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu13079	XM_006539854.3 Latest version!	Mus musculus B9 protein domain 2 (B9d2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OMu13079	XM_006539854.2	Mus musculus B9 protein domain 2 (B9d2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu13079	NM_172148.1	Mus musculus B9 protein domain 2 (B9d2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

B9d2 ( NM_172148.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_742160.1 Mus musculus B9 protein domain 2 (B9d2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu13079
Clone ID Related Accession (Same CDS sequence)	XM_006539854.3 , NM_172148.2 , XM_006539854.2 , NM_172148.1 , NM_001362154.1
Accession Version	NM_172148.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-01
Organism	Mus musculus(house mouse)
Product	B9 domain-containing protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC028440.1 and BX517802.1. On Apr 6, 2018 this sequence version replaced NM_172148.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC028440.1, SRR1660819.271342.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGAAG TGCACGTGAT CGGGCAGATC ATAGGGGCCA CCGGTTTCTC GGAAAGCAGC 
CTCTTTTGCA AGTGGGGCAT CCACACAGGG GCCGCATGGA AGCTCCTCTC AGGTGTACGA 
GAAGGCCAGA CACAGGTAGA CACACCCCAG ATAGGGGACA TGGCTTACTG GTCCCACCCC 
ATTGACCTGC ACTTCGCTAC TAAAGGTCTC CAAGGTTGGC CTCGACTCCA TCTCCAGGTG 
TGGTCCCAGG ACAGCTTTGG CCGCTGCCAG CTTGCTGGCT ATGGCTTTTG CCATGTGCCT 
AGCAGCCCAG GCACTCATCA GCTGGACTGC CCTACATGGA GGCCCCTGGG CAGTTGGAGG 
GAGCAGCTAG CACGGGCTTT CGTGGGTGGT GGGCCACAGC TGCTGCACGC AGATACCATC 
TACAGCGGGG CTGACCGCTA CCGCCTGCAC ACGGCCGCGG GTGGCACAGT GCACCTTGGT 
ATCGGCCTGC TGCTGCGCCA CTTCGATCGC TATGGAGTGG AGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_742160.1
CDS	230..757
Translation	MAEVHVIGQIIGATGFSESSLFCKWGIHTGAAWKLLSGVREGQTQVDTPQIGDMAYWSHPIDLHFATKGLQGWPRLHLQVWSQDSFGRCQLAGYGFCHVPSSPGTHQLDCPTWRPLGSWREQLARAFVGGGPQLLHADTIYSGADRYRLHTAAGGTVHLGIGLLLRHFDRYGVEC

Target ORF information:

RefSeq Version	NM_172148.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus B9 protein domain 2 (B9d2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172148.2

ORF Insert Sequence:

ATGGCTGAAG TGCACGTGAT CGGGCAGATC ATAGGGGCCA CCGGTTTCTC GGAAAGCAGC 
CTCTTTTGCA AGTGGGGCAT CCACACAGGG GCCGCATGGA AGCTCCTCTC AGGTGTACGA 
GAAGGCCAGA CACAGGTAGA CACACCCCAG ATAGGGGACA TGGCTTACTG GTCCCACCCC 
ATTGACCTGC ACTTCGCTAC TAAAGGTCTC CAAGGTTGGC CTCGACTCCA TCTCCAGGTG 
TGGTCCCAGG ACAGCTTTGG CCGCTGCCAG CTTGCTGGCT ATGGCTTTTG CCATGTGCCT 
AGCAGCCCAG GCACTCATCA GCTGGACTGC CCTACATGGA GGCCCCTGGG CAGTTGGAGG 
GAGCAGCTAG CACGGGCTTT CGTGGGTGGT GGGCCACAGC TGCTGCACGC AGATACCATC 
TACAGCGGGG CTGACCGCTA CCGCCTGCAC ACGGCCGCGG GTGGCACAGT GCACCTTGGT 
ATCGGCCTGC TGCTGCGCCA CTTCGATCGC TATGGAGTGG AGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B9d2 ( NM_172148.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_742160.1 Mus musculus B9 protein domain 2 (B9d2), mRNA.

Price & Availability↑

CloneID	OMu13079
Clone ID Related Accession (Same CDS sequence)	XM_006539854.3 , NM_172148.2 , XM_006539854.2 , NM_172148.1 , NM_001362154.1
Accession Version	NM_172148.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-09
Organism	Mus musculus(house mouse)
Product	B9 domain-containing protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC028440.1. On or before May 4, 2006 this sequence version replaced XM_989711.1, XM_989751.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC028440.1, SRR1660819.271342.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGAAG TGCACGTGAT CGGGCAGATC ATAGGGGCCA CCGGTTTCTC GGAAAGCAGC 
CTCTTTTGCA AGTGGGGCAT CCACACAGGG GCCGCATGGA AGCTCCTCTC AGGTGTACGA 
GAAGGCCAGA CACAGGTAGA CACACCCCAG ATAGGGGACA TGGCTTACTG GTCCCACCCC 
ATTGACCTGC ACTTCGCTAC TAAAGGTCTC CAAGGTTGGC CTCGACTCCA TCTCCAGGTG 
TGGTCCCAGG ACAGCTTTGG CCGCTGCCAG CTTGCTGGCT ATGGCTTTTG CCATGTGCCT 
AGCAGCCCAG GCACTCATCA GCTGGACTGC CCTACATGGA GGCCCCTGGG CAGTTGGAGG 
GAGCAGCTAG CACGGGCTTT CGTGGGTGGT GGGCCACAGC TGCTGCACGC AGATACCATC 
TACAGCGGGG CTGACCGCTA CCGCCTGCAC ACGGCCGCGG GTGGCACAGT GCACCTTGGT 
ATCGGCCTGC TGCTGCGCCA CTTCGATCGC TATGGAGTGG AGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_742160.1
CDS	230..757
Translation	MAEVHVIGQIIGATGFSESSLFCKWGIHTGAAWKLLSGVREGQTQVDTPQIGDMAYWSHPIDLHFATKGLQGWPRLHLQVWSQDSFGRCQLAGYGFCHVPSSPGTHQLDCPTWRPLGSWREQLARAFVGGGPQLLHADTIYSGADRYRLHTAAGGTVHLGIGLLLRHFDRYGVEC

Target ORF information:

RefSeq Version	NM_172148.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus B9 protein domain 2 (B9d2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172148.1

ORF Insert Sequence:

ATGGCTGAAG TGCACGTGAT CGGGCAGATC ATAGGGGCCA CCGGTTTCTC GGAAAGCAGC 
CTCTTTTGCA AGTGGGGCAT CCACACAGGG GCCGCATGGA AGCTCCTCTC AGGTGTACGA 
GAAGGCCAGA CACAGGTAGA CACACCCCAG ATAGGGGACA TGGCTTACTG GTCCCACCCC 
ATTGACCTGC ACTTCGCTAC TAAAGGTCTC CAAGGTTGGC CTCGACTCCA TCTCCAGGTG 
TGGTCCCAGG ACAGCTTTGG CCGCTGCCAG CTTGCTGGCT ATGGCTTTTG CCATGTGCCT 
AGCAGCCCAG GCACTCATCA GCTGGACTGC CCTACATGGA GGCCCCTGGG CAGTTGGAGG 
GAGCAGCTAG CACGGGCTTT CGTGGGTGGT GGGCCACAGC TGCTGCACGC AGATACCATC 
TACAGCGGGG CTGACCGCTA CCGCCTGCAC ACGGCCGCGG GTGGCACAGT GCACCTTGGT 
ATCGGCCTGC TGCTGCGCCA CTTCGATCGC TATGGAGTGG AGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B9d2 ( NM_001362154.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001349083.1 Mus musculus B9 protein domain 2 (B9d2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13079
Clone ID Related Accession (Same CDS sequence)	XM_006539854.3 , NM_172148.2 , XM_006539854.2 , NM_172148.1 , NM_001362154.1
Accession Version	NM_001362154.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	B9 domain-containing protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK141500.1 and BX517802.1. On Apr 6, 2018 this sequence version replaced XM_006539854.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK141500.1, SRR1660815.94621.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGAAG TGCACGTGAT CGGGCAGATC ATAGGGGCCA CCGGTTTCTC GGAAAGCAGC 
CTCTTTTGCA AGTGGGGCAT CCACACAGGG GCCGCATGGA AGCTCCTCTC AGGTGTACGA 
GAAGGCCAGA CACAGGTAGA CACACCCCAG ATAGGGGACA TGGCTTACTG GTCCCACCCC 
ATTGACCTGC ACTTCGCTAC TAAAGGTCTC CAAGGTTGGC CTCGACTCCA TCTCCAGGTG 
TGGTCCCAGG ACAGCTTTGG CCGCTGCCAG CTTGCTGGCT ATGGCTTTTG CCATGTGCCT 
AGCAGCCCAG GCACTCATCA GCTGGACTGC CCTACATGGA GGCCCCTGGG CAGTTGGAGG 
GAGCAGCTAG CACGGGCTTT CGTGGGTGGT GGGCCACAGC TGCTGCACGC AGATACCATC 
TACAGCGGGG CTGACCGCTA CCGCCTGCAC ACGGCCGCGG GTGGCACAGT GCACCTTGGT 
ATCGGCCTGC TGCTGCGCCA CTTCGATCGC TATGGAGTGG AGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349083.1
CDS	204..731
Translation	MAEVHVIGQIIGATGFSESSLFCKWGIHTGAAWKLLSGVREGQTQVDTPQIGDMAYWSHPIDLHFATKGLQGWPRLHLQVWSQDSFGRCQLAGYGFCHVPSSPGTHQLDCPTWRPLGSWREQLARAFVGGGPQLLHADTIYSGADRYRLHTAAGGTVHLGIGLLLRHFDRYGVEC

Target ORF information:

RefSeq Version	NM_001362154.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus B9 protein domain 2 (B9d2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362154.1

ORF Insert Sequence:

ATGGCTGAAG TGCACGTGAT CGGGCAGATC ATAGGGGCCA CCGGTTTCTC GGAAAGCAGC 
CTCTTTTGCA AGTGGGGCAT CCACACAGGG GCCGCATGGA AGCTCCTCTC AGGTGTACGA 
GAAGGCCAGA CACAGGTAGA CACACCCCAG ATAGGGGACA TGGCTTACTG GTCCCACCCC 
ATTGACCTGC ACTTCGCTAC TAAAGGTCTC CAAGGTTGGC CTCGACTCCA TCTCCAGGTG 
TGGTCCCAGG ACAGCTTTGG CCGCTGCCAG CTTGCTGGCT ATGGCTTTTG CCATGTGCCT 
AGCAGCCCAG GCACTCATCA GCTGGACTGC CCTACATGGA GGCCCCTGGG CAGTTGGAGG 
GAGCAGCTAG CACGGGCTTT CGTGGGTGGT GGGCCACAGC TGCTGCACGC AGATACCATC 
TACAGCGGGG CTGACCGCTA CCGCCTGCAC ACGGCCGCGG GTGGCACAGT GCACCTTGGT 
ATCGGCCTGC TGCTGCGCCA CTTCGATCGC TATGGAGTGG AGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B9d2 ( XM_006539854.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539917.1 Mus musculus B9 protein domain 2 (B9d2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu13079
Clone ID Related Accession (Same CDS sequence)	XM_006539854.3 , NM_172148.2 , XM_006539854.2 , NM_172148.1 , NM_001362154.1
Accession Version	XM_006539854.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	B9 domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539854.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAAG TGCACGTGAT CGGGCAGATC ATAGGGGCCA CCGGTTTCTC GGAAAGCAGC 
CTCTTTTGCA AGTGGGGCAT CCACACAGGG GCCGCATGGA AGCTCCTCTC AGGTGTACGA 
GAAGGCCAGA CACAGGTAGA CACACCCCAG ATAGGGGACA TGGCTTACTG GTCCCACCCC 
ATTGACCTGC ACTTCGCTAC TAAAGGTCTC CAAGGTTGGC CTCGACTCCA TCTCCAGGTG 
TGGTCCCAGG ACAGCTTTGG CCGCTGCCAG CTTGCTGGCT ATGGCTTTTG CCATGTGCCT 
AGCAGCCCAG GCACTCATCA GCTGGACTGC CCTACATGGA GGCCCCTGGG CAGTTGGAGG 
GAGCAGCTAG CACGGGCTTT CGTGGGTGGT GGGCCACAGC TGCTGCACGC AGATACCATC 
TACAGCGGGG CTGACCGCTA CCGCCTGCAC ACGGCCGCGG GTGGCACAGT GCACCTTGGT 
ATCGGCCTGC TGCTGCGCCA CTTCGATCGC TATGGAGTGG AGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539917.1
CDS	1313..1840
Translation	MAEVHVIGQIIGATGFSESSLFCKWGIHTGAAWKLLSGVREGQTQVDTPQIGDMAYWSHPIDLHFATKGLQGWPRLHLQVWSQDSFGRCQLAGYGFCHVPSSPGTHQLDCPTWRPLGSWREQLARAFVGGGPQLLHADTIYSGADRYRLHTAAGGTVHLGIGLLLRHFDRYGVEC

Target ORF information:

RefSeq Version	XM_006539854.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus B9 protein domain 2 (B9d2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539854.3

ORF Insert Sequence:

ATGGCTGAAG TGCACGTGAT CGGGCAGATC ATAGGGGCCA CCGGTTTCTC GGAAAGCAGC 
CTCTTTTGCA AGTGGGGCAT CCACACAGGG GCCGCATGGA AGCTCCTCTC AGGTGTACGA 
GAAGGCCAGA CACAGGTAGA CACACCCCAG ATAGGGGACA TGGCTTACTG GTCCCACCCC 
ATTGACCTGC ACTTCGCTAC TAAAGGTCTC CAAGGTTGGC CTCGACTCCA TCTCCAGGTG 
TGGTCCCAGG ACAGCTTTGG CCGCTGCCAG CTTGCTGGCT ATGGCTTTTG CCATGTGCCT 
AGCAGCCCAG GCACTCATCA GCTGGACTGC CCTACATGGA GGCCCCTGGG CAGTTGGAGG 
GAGCAGCTAG CACGGGCTTT CGTGGGTGGT GGGCCACAGC TGCTGCACGC AGATACCATC 
TACAGCGGGG CTGACCGCTA CCGCCTGCAC ACGGCCGCGG GTGGCACAGT GCACCTTGGT 
ATCGGCCTGC TGCTGCGCCA CTTCGATCGC TATGGAGTGG AGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B9d2 ( XM_006539854.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539917.1 Mus musculus B9 protein domain 2 (B9d2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu13079
Clone ID Related Accession (Same CDS sequence)	XM_006539854.3 , NM_172148.2 , XM_006539854.2 , NM_172148.1 , NM_001362154.1
Accession Version	XM_006539854.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	B9 domain-containing protein 2 isoform X1
Comment

ATGGCTGAAG TGCACGTGAT CGGGCAGATC ATAGGGGCCA CCGGTTTCTC GGAAAGCAGC 
CTCTTTTGCA AGTGGGGCAT CCACACAGGG GCCGCATGGA AGCTCCTCTC AGGTGTACGA 
GAAGGCCAGA CACAGGTAGA CACACCCCAG ATAGGGGACA TGGCTTACTG GTCCCACCCC 
ATTGACCTGC ACTTCGCTAC TAAAGGTCTC CAAGGTTGGC CTCGACTCCA TCTCCAGGTG 
TGGTCCCAGG ACAGCTTTGG CCGCTGCCAG CTTGCTGGCT ATGGCTTTTG CCATGTGCCT 
AGCAGCCCAG GCACTCATCA GCTGGACTGC CCTACATGGA GGCCCCTGGG CAGTTGGAGG 
GAGCAGCTAG CACGGGCTTT CGTGGGTGGT GGGCCACAGC TGCTGCACGC AGATACCATC 
TACAGCGGGG CTGACCGCTA CCGCCTGCAC ACGGCCGCGG GTGGCACAGT GCACCTTGGT 
ATCGGCCTGC TGCTGCGCCA CTTCGATCGC TATGGAGTGG AGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539917.1
Translation	MAEVHVIGQIIGATGFSESSLFCKWGIHTGAAWKLLSGVREGQTQVDTPQIGDMAYWSHPIDLHFATKGLQGWPRLHLQVWSQDSFGRCQLAGYGFCHVPSSPGTHQLDCPTWRPLGSWREQLARAFVGGGPQLLHADTIYSGADRYRLHTAAGGTVHLGIGLLLRHFDRYGVEC

Target ORF information:

RefSeq Version	XM_006539854.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus B9 protein domain 2 (B9d2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539854.2

ORF Insert Sequence:

ATGGCTGAAG TGCACGTGAT CGGGCAGATC ATAGGGGCCA CCGGTTTCTC GGAAAGCAGC 
CTCTTTTGCA AGTGGGGCAT CCACACAGGG GCCGCATGGA AGCTCCTCTC AGGTGTACGA 
GAAGGCCAGA CACAGGTAGA CACACCCCAG ATAGGGGACA TGGCTTACTG GTCCCACCCC 
ATTGACCTGC ACTTCGCTAC TAAAGGTCTC CAAGGTTGGC CTCGACTCCA TCTCCAGGTG 
TGGTCCCAGG ACAGCTTTGG CCGCTGCCAG CTTGCTGGCT ATGGCTTTTG CCATGTGCCT 
AGCAGCCCAG GCACTCATCA GCTGGACTGC CCTACATGGA GGCCCCTGGG CAGTTGGAGG 
GAGCAGCTAG CACGGGCTTT CGTGGGTGGT GGGCCACAGC TGCTGCACGC AGATACCATC 
TACAGCGGGG CTGACCGCTA CCGCCTGCAC ACGGCCGCGG GTGGCACAGT GCACCTTGGT 
ATCGGCCTGC TGCTGCGCCA CTTCGATCGC TATGGAGTGG AGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The GARP/Latent TGF-?1 complex on Treg cells modulates the induction of peripherally derived Treg cells during oral tolerance.
European journal of immunology46(6)1480-9(2016 06)
Edwards JP,Hand TW,Morais da Fonseca D,Glass DD,Belkaid Y,Shevach EM

Generation of Th17 cells in response to intranasal infection requires TGF-?1 from dendritic cells and IL-6 from CD301b+ dendritic cells.
Proceedings of the National Academy of Sciences of the United States of America112(41)12782-7(2015 Oct)
Linehan JL,Dileepan T,Kashem SW,Kaplan DH,Cleary P,Jenkins MK

Release of active TGF-?1 from the latent TGF-?1/GARP complex on T regulatory cells is mediated by integrin ?8.
Journal of immunology (Baltimore, Md. : 1950)193(6)2843-9(2014 Sep)
Edwards JP,Thornton AM,Shevach EM

Macrophage-specific deletion of transforming growth factor-?1 does not prevent renal fibrosis after severe ischemia-reperfusion or obstructive injury.
American journal of physiology. Renal physiology305(4)F477-84(2013 Aug)
Huen SC,Moeckel GW,Cantley LG

Regulation of the expression of GARP/latent TGF-?1 complexes on mouse T cells and their role in regulatory T cell and Th17 differentiation.
Journal of immunology (Baltimore, Md. : 1950)190(11)5506-15(2013 Jun)
Edwards JP,Fujii H,Zhou AX,Creemers J,Unutmaz D,Shevach EM