B9D2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	B9D2
Entrez Gene ID	80776
Full Name	B9 domain containing 2
Synonyms	ICIS-1,JBTS34,MKS10,MKSR2
General protein information	Preferred Names B9 domain containing 2 Names B9 domain-containing protein 2 B9 protein domain 2 MKS1-related protein 2 involved in cIlia stability-1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.2
Summary	This gene encodes a B9 domain protein, which are exclusively found in ciliated organisms. The gene is upregulated during mucociliary differentiation, and the encoded protein localizes to basal bodies and cilia. Disrupting expression of this gene results in ciliogenesis defects. [provided by RefSeq, Oct 2009].
Disorder MIM:	611951
Disorder Html:	?Meckel syndrome 10, 614175 (3); Joubert syndrome 34, 614175 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_030578.3	NP_085055.2	B9 domain-containing protein 2
XM_011527350.2	XP_011525652.1	B9 domain-containing protein 2 isoform X2
NM_030578.4	NP_085055.2	B9 domain-containing protein 2
XM_011527350.1	XP_011525652.1	B9 domain-containing protein 2 isoform X2
XM_011527349.2	XP_011525651.1	B9 domain-containing protein 2 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	B9d2	NP_001100964.1
Bos taurus (cattle)	B9D2	NP_001035683.1
Mus musculus (house mouse)	B9d2	NP_742160.1
Drosophila melanogaster (fruit fly)	CG42730	NP_001188704.1
Caenorhabditis elegans (roundworm)	mksr-2	NP_500186.1
	B9D2	XP_001101041.1
Canis lupus familiaris (dog)	B9D2	XP_533658.1
Homo sapiens (human)	B9D2	NP_085055.2
Pan troglodytes (chimpanzee)	B9D2	XP_001140469.1
Danio rerio (zebrafish)	b9d2	NP_001002394.1
Xenopus tropicalis (tropical clawed frog)	b9d2	XP_002939434.1

Gene Ontology
Bibliography

Related articles in PubMed

An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.
Boldt K, van Reeuwijk J, Lu Q, Koutroumpas K, Nguyen TM, Texier Y, van Beersum SE, Horn N, Willer JR, Mans DA, Dougherty G, Lamers IJ, Coene KL, Arts HH, Betts MJ, Beyer T, Bolat E, Gloeckner CJ, Haidari K, Hetterschijt L, Iaconis D, Jenkins D, Klose F, Knapp B, Latour B, Letteboer SJ, Marcelis CL, Mitic D, Morleo M, Oud MM, Riemersma M, Rix S, Terhal PA, Toedt G, van Dam TJ, de Vrieze E, Wissinger Y, Wu KM, Apic G, Beales PL, Blacque OE, Gibson TJ, Huynen MA, Katsanis N, Kremer H, Omran H, van Wijk E, Wolfrum U, Kepes F, Davis EE, Franco B, Giles RH, Ueffing M, Russell RB, Roepman R,
Nature communications711491(2016 May)

A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface.
Gupta GD, Coyaud ?, Gon?alves J, Mojarad BA, Liu Y, Wu Q, Gheiratmand L, Comartin D, Tkach JM, Cheung SW, Bashkurov M, Hasegan M, Knight JD, Lin ZY, Schueler M, Hildebrandt F, Moffat J, Gingras AC, Raught B, Pelletier L
Cell163(6)1484-99(2015 Dec)

Large-scale genetic study in East Asians identifies six new loci associated with colorectal cancer risk.
Zhang B, Jia WH, Matsuda K, Kweon SS, Matsuo K, Xiang YB, Shin A, Jee SH, Kim DH, Cai Q, Long J, Shi J, Wen W, Yang G, Zhang Y, Li C, Li B, Guo Y, Ren Z, Ji BT, Pan ZZ, Takahashi A, Shin MH, Matsuda F, Gao YT, Oh JH, Kim S, Ahn YO, , Chan AT, Chang-Claude J, Slattery ML, , Gruber SB, Schumacher FR, Stenzel SL, , Casey G, Kim HR, Jeong JY, Park JW, Li HL, Hosono S, Cho SH, Kubo M, Shu XO, Zeng YX, Zheng W
Nature genetics46(6)533-42(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: Inflammation gene variants and susceptibility to albuminuria in the U.S. population: analysis in the Third National Health and Nutrition Examination Survey (NHANES III), 1991-1994.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic susceptibility to distinct bladder cancer subphenotypes.

MKS-1 and MKS-1-related proteins 1 and 2 (MKSR-1/EPPB9, MKSR-2/B9D2), localize to transition zones/basal bodies of sensory cilia; subcellular localization is largely co-dependent, pointing to a functional relationship between the proteins
Title: Functional interactions between the ciliopathy-associated Meckel syndrome 1 (MKS1) protein and two novel MKS1-related (MKSR) proteins.

Observational study of genotype prevalence. (HuGE Navigator)
Title: Prevalence in the United States of selected candidate gene variants: Third National Health and Nutrition Examination Survey, 1991-1994.

The ICIS-1 (Involved in CIlia Stability-1)gene is localized within the TGF-beta1 promoter and is upregulated during mucociliary differentiation.
Title: Identification of ICIS-1, a new protein involved in cilia stability.

The following B9D2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the B9D2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26216	NM_030578.4 Latest version!	Homo sapiens B9 domain containing 2 (B9D2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu26216	XM_011527349.2 Latest version!	Homo sapiens B9 domain containing 2 (B9D2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu72835	XM_011527350.2 Latest version!	Homo sapiens B9 domain containing 2 (B9D2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OHu26216	NM_030578.3	Homo sapiens B9 domain containing 2 (B9D2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu72835	XM_011527350.1	Homo sapiens B9 protein domain 2 (B9D2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

B9D2 ( NM_030578.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_085055.2 Homo sapiens B9 domain containing 2 (B9D2), mRNA.

Price & Availability↑

CloneID	OHu26216
Clone ID Related Accession (Same CDS sequence)	NM_030578.3 , NM_030578.4 , XM_011527349.2
Accession Version	NM_030578.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-19
Organism	Homo sapiens(human)
Product	B9 domain-containing protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA196504.1, BM833644.1, BC004157.2 and BU609059.1. This sequence is a reference standard in the RefSeqGene project. On Apr 4, 2009 this sequence version replaced NM_030578.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BF364906.1, SRR1163657.38730.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGAGG TGCACGTGAT CGGGCAGATC ATAGGGGCCA GCGGTTTCTC GGAAAGTAGC 
CTCTTCTGCA AGTGGGGCAT TCACACAGGG GCGGCATGGA AGCTCCTGTC AGGCGTGCGG 
GAGGGCCAAA CGCAAGTGGA CACCCCGCAG ATAGGGGACA TGGCTTACTG GTCCCACCCC 
ATCGACCTGC ACTTCGCCAC CAAAGGTCTT CAAGGCTGGC CCCGGCTCCA TTTCCAGGTG 
TGGTCCCAGG ACAGCTTTGG CCGCTGCCAG CTTGCAGGCT ATGGATTTTG CCATGTGCCC 
AGTAGCCCGG GCACCCACCA GCTGGCCTGC CCCACGTGGC GGCCCCTGGG CAGTTGGCGA 
GAACAGTTGG CACGGGCTTT CGTGGGTGGT GGGCCGCAGC TGCTGCATGG GGACACCATC 
TACAGTGGGG CCGACCGCTA TCGCCTGCAC ACAGCTGCTG GTGGCACCGT GCACCTGGAG 
ATCGGCCTGC TGCTCCGCAA CTTCGACCGC TACGGCGTGG AGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_085055.2
CDS	221..748
Translation	MAEVHVIGQIIGASGFSESSLFCKWGIHTGAAWKLLSGVREGQTQVDTPQIGDMAYWSHPIDLHFATKGLQGWPRLHFQVWSQDSFGRCQLAGYGFCHVPSSPGTHQLACPTWRPLGSWREQLARAFVGGGPQLLHGDTIYSGADRYRLHTAAGGTVHLEIGLLLRNFDRYGVEC

Target ORF information:

RefSeq Version	NM_030578.3
Organism	Homo sapiens(human)
Definition	Homo sapiens B9 domain containing 2 (B9D2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030578.3

ORF Insert Sequence:

ATGGCTGAGG TGCACGTGAT CGGGCAGATC ATAGGGGCCA GCGGTTTCTC GGAAAGTAGC 
CTCTTCTGCA AGTGGGGCAT TCACACAGGG GCGGCATGGA AGCTCCTGTC AGGCGTGCGG 
GAGGGCCAAA CGCAAGTGGA CACCCCGCAG ATAGGGGACA TGGCTTACTG GTCCCACCCC 
ATCGACCTGC ACTTCGCCAC CAAAGGTCTT CAAGGCTGGC CCCGGCTCCA TTTCCAGGTG 
TGGTCCCAGG ACAGCTTTGG CCGCTGCCAG CTTGCAGGCT ATGGATTTTG CCATGTGCCC 
AGTAGCCCGG GCACCCACCA GCTGGCCTGC CCCACGTGGC GGCCCCTGGG CAGTTGGCGA 
GAACAGTTGG CACGGGCTTT CGTGGGTGGT GGGCCGCAGC TGCTGCATGG GGACACCATC 
TACAGTGGGG CCGACCGCTA TCGCCTGCAC ACAGCTGCTG GTGGCACCGT GCACCTGGAG 
ATCGGCCTGC TGCTCCGCAA CTTCGACCGC TACGGCGTGG AGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B9D2 ( NM_030578.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_085055.2 Homo sapiens B9 domain containing 2 (B9D2), mRNA.

Price & Availability↑

CloneID	OHu26216
Clone ID Related Accession (Same CDS sequence)	NM_030578.3 , NM_030578.4 , XM_011527349.2
Accession Version	NM_030578.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	B9 domain-containing protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA196504.1, BM833644.1, BC004157.2 and BU609059.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_030578.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.38730.1, SRR5189652.35644.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000243578.8/ ENSP00000243578.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTGAGG TGCACGTGAT CGGGCAGATC ATAGGGGCCA GCGGTTTCTC GGAAAGTAGC 
CTCTTCTGCA AGTGGGGCAT TCACACAGGG GCGGCATGGA AGCTCCTGTC AGGCGTGCGG 
GAGGGCCAAA CGCAAGTGGA CACCCCGCAG ATAGGGGACA TGGCTTACTG GTCCCACCCC 
ATCGACCTGC ACTTCGCCAC CAAAGGTCTT CAAGGCTGGC CCCGGCTCCA TTTCCAGGTG 
TGGTCCCAGG ACAGCTTTGG CCGCTGCCAG CTTGCAGGCT ATGGATTTTG CCATGTGCCC 
AGTAGCCCGG GCACCCACCA GCTGGCCTGC CCCACGTGGC GGCCCCTGGG CAGTTGGCGA 
GAACAGTTGG CACGGGCTTT CGTGGGTGGT GGGCCGCAGC TGCTGCATGG GGACACCATC 
TACAGTGGGG CCGACCGCTA TCGCCTGCAC ACAGCTGCTG GTGGCACCGT GCACCTGGAG 
ATCGGCCTGC TGCTCCGCAA CTTCGACCGC TACGGCGTGG AGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_085055.2
CDS	197..724
Translation	MAEVHVIGQIIGASGFSESSLFCKWGIHTGAAWKLLSGVREGQTQVDTPQIGDMAYWSHPIDLHFATKGLQGWPRLHFQVWSQDSFGRCQLAGYGFCHVPSSPGTHQLACPTWRPLGSWREQLARAFVGGGPQLLHGDTIYSGADRYRLHTAAGGTVHLEIGLLLRNFDRYGVEC

Target ORF information:

RefSeq Version	NM_030578.4
Organism	Homo sapiens(human)
Definition	Homo sapiens B9 domain containing 2 (B9D2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030578.4

ORF Insert Sequence:

ATGGCTGAGG TGCACGTGAT CGGGCAGATC ATAGGGGCCA GCGGTTTCTC GGAAAGTAGC 
CTCTTCTGCA AGTGGGGCAT TCACACAGGG GCGGCATGGA AGCTCCTGTC AGGCGTGCGG 
GAGGGCCAAA CGCAAGTGGA CACCCCGCAG ATAGGGGACA TGGCTTACTG GTCCCACCCC 
ATCGACCTGC ACTTCGCCAC CAAAGGTCTT CAAGGCTGGC CCCGGCTCCA TTTCCAGGTG 
TGGTCCCAGG ACAGCTTTGG CCGCTGCCAG CTTGCAGGCT ATGGATTTTG CCATGTGCCC 
AGTAGCCCGG GCACCCACCA GCTGGCCTGC CCCACGTGGC GGCCCCTGGG CAGTTGGCGA 
GAACAGTTGG CACGGGCTTT CGTGGGTGGT GGGCCGCAGC TGCTGCATGG GGACACCATC 
TACAGTGGGG CCGACCGCTA TCGCCTGCAC ACAGCTGCTG GTGGCACCGT GCACCTGGAG 
ATCGGCCTGC TGCTCCGCAA CTTCGACCGC TACGGCGTGG AGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B9D2 ( XM_011527349.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011525651.1 Homo sapiens B9 domain containing 2 (B9D2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu26216
Clone ID Related Accession (Same CDS sequence)	NM_030578.3 , NM_030578.4 , XM_011527349.2
Accession Version	XM_011527349.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	B9 domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011527349.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG TGCACGTGAT CGGGCAGATC ATAGGGGCCA GCGGTTTCTC GGAAAGTAGC 
CTCTTCTGCA AGTGGGGCAT TCACACAGGG GCGGCATGGA AGCTCCTGTC AGGCGTGCGG 
GAGGGCCAAA CGCAAGTGGA CACCCCGCAG ATAGGGGACA TGGCTTACTG GTCCCACCCC 
ATCGACCTGC ACTTCGCCAC CAAAGGTCTT CAAGGCTGGC CCCGGCTCCA TTTCCAGGTG 
TGGTCCCAGG ACAGCTTTGG CCGCTGCCAG CTTGCAGGCT ATGGATTTTG CCATGTGCCC 
AGTAGCCCGG GCACCCACCA GCTGGCCTGC CCCACGTGGC GGCCCCTGGG CAGTTGGCGA 
GAACAGTTGG CACGGGCTTT CGTGGGTGGT GGGCCGCAGC TGCTGCATGG GGACACCATC 
TACAGTGGGG CCGACCGCTA TCGCCTGCAC ACAGCTGCTG GTGGCACCGT GCACCTGGAG 
ATCGGCCTGC TGCTCCGCAA CTTCGACCGC TACGGCGTGG AGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011525651.1
CDS	172..699
Translation	MAEVHVIGQIIGASGFSESSLFCKWGIHTGAAWKLLSGVREGQTQVDTPQIGDMAYWSHPIDLHFATKGLQGWPRLHFQVWSQDSFGRCQLAGYGFCHVPSSPGTHQLACPTWRPLGSWREQLARAFVGGGPQLLHGDTIYSGADRYRLHTAAGGTVHLEIGLLLRNFDRYGVEC

Target ORF information:

RefSeq Version	XM_011527349.2
Organism	Homo sapiens(human)
Definition	Homo sapiens B9 domain containing 2 (B9D2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527349.2

ORF Insert Sequence:

ATGGCTGAGG TGCACGTGAT CGGGCAGATC ATAGGGGCCA GCGGTTTCTC GGAAAGTAGC 
CTCTTCTGCA AGTGGGGCAT TCACACAGGG GCGGCATGGA AGCTCCTGTC AGGCGTGCGG 
GAGGGCCAAA CGCAAGTGGA CACCCCGCAG ATAGGGGACA TGGCTTACTG GTCCCACCCC 
ATCGACCTGC ACTTCGCCAC CAAAGGTCTT CAAGGCTGGC CCCGGCTCCA TTTCCAGGTG 
TGGTCCCAGG ACAGCTTTGG CCGCTGCCAG CTTGCAGGCT ATGGATTTTG CCATGTGCCC 
AGTAGCCCGG GCACCCACCA GCTGGCCTGC CCCACGTGGC GGCCCCTGGG CAGTTGGCGA 
GAACAGTTGG CACGGGCTTT CGTGGGTGGT GGGCCGCAGC TGCTGCATGG GGACACCATC 
TACAGTGGGG CCGACCGCTA TCGCCTGCAC ACAGCTGCTG GTGGCACCGT GCACCTGGAG 
ATCGGCCTGC TGCTCCGCAA CTTCGACCGC TACGGCGTGG AGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B9D2 ( XM_011527350.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011525652.1 Homo sapiens B9 domain containing 2 (B9D2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu72835
Clone ID Related Accession (Same CDS sequence)	XM_011527350.2 , XM_011527350.1
Accession Version	XM_011527350.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	B9 domain-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011527350.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTACT GGTCCCACCC CATCGACCTG CACTTCGCCA CCAAAGGTCT TCAAGGCTGG 
CCCCGGCTCC ATTTCCAGGT GTGGTCCCAG GACAGCTTTG GCCGCTGCCA GCTTGCAGGC 
TATGGATTTT GCCATGTGCC CAGTAGCCCG GGCACCCACC AGCTGGCCTG CCCCACGTGG 
CGGCCCCTGG GCAGTTGGCG AGAACAGTTG GCACGGGCTT TCGTGGGTGG TGGGCCGCAG 
CTGCTGCATG GGGACACCAT CTACAGTGGG GCCGACCGCT ATCGCCTGCA CACAGCTGCT 
GGTGGCACCG TGCACCTGGA GATCGGCCTG CTGCTCCGCA ACTTCGACCG CTACGGCGTG 
GAGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011525652.1
CDS	649..1017
Translation	MAYWSHPIDLHFATKGLQGWPRLHFQVWSQDSFGRCQLAGYGFCHVPSSPGTHQLACPTWRPLGSWREQLARAFVGGGPQLLHGDTIYSGADRYRLHTAAGGTVHLEIGLLLRNFDRYGVEC

Target ORF information:

RefSeq Version	XM_011527350.2
Organism	Homo sapiens(human)
Definition	Homo sapiens B9 domain containing 2 (B9D2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527350.2

ORF Insert Sequence:

ATGGCTTACT GGTCCCACCC CATCGACCTG CACTTCGCCA CCAAAGGTCT TCAAGGCTGG 
CCCCGGCTCC ATTTCCAGGT GTGGTCCCAG GACAGCTTTG GCCGCTGCCA GCTTGCAGGC 
TATGGATTTT GCCATGTGCC CAGTAGCCCG GGCACCCACC AGCTGGCCTG CCCCACGTGG 
CGGCCCCTGG GCAGTTGGCG AGAACAGTTG GCACGGGCTT TCGTGGGTGG TGGGCCGCAG 
CTGCTGCATG GGGACACCAT CTACAGTGGG GCCGACCGCT ATCGCCTGCA CACAGCTGCT 
GGTGGCACCG TGCACCTGGA GATCGGCCTG CTGCTCCGCA ACTTCGACCG CTACGGCGTG 
GAGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B9D2 ( XM_011527350.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011525652.1 Homo sapiens B9 protein domain 2 (B9D2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu72835
Clone ID Related Accession (Same CDS sequence)	XM_011527350.2 , XM_011527350.1
Accession Version	XM_011527350.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	B9 domain-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTACT GGTCCCACCC CATCGACCTG CACTTCGCCA CCAAAGGTCT TCAAGGCTGG 
CCCCGGCTCC ATTTCCAGGT GTGGTCCCAG GACAGCTTTG GCCGCTGCCA GCTTGCAGGC 
TATGGATTTT GCCATGTGCC CAGTAGCCCG GGCACCCACC AGCTGGCCTG CCCCACGTGG 
CGGCCCCTGG GCAGTTGGCG AGAACAGTTG GCACGGGCTT TCGTGGGTGG TGGGCCGCAG 
CTGCTGCATG GGGACACCAT CTACAGTGGG GCCGACCGCT ATCGCCTGCA CACAGCTGCT 
GGTGGCACCG TGCACCTGGA GATCGGCCTG CTGCTCCGCA ACTTCGACCG CTACGGCGTG 
GAGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011525652.1
CDS	655..1023
Translation	MAYWSHPIDLHFATKGLQGWPRLHFQVWSQDSFGRCQLAGYGFCHVPSSPGTHQLACPTWRPLGSWREQLARAFVGGGPQLLHGDTIYSGADRYRLHTAAGGTVHLEIGLLLRNFDRYGVEC

Target ORF information:

RefSeq Version	XM_011527350.1
Organism	Homo sapiens(human)
Definition	Homo sapiens B9 protein domain 2 (B9D2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527350.1

ORF Insert Sequence:

ATGGCTTACT GGTCCCACCC CATCGACCTG CACTTCGCCA CCAAAGGTCT TCAAGGCTGG 
CCCCGGCTCC ATTTCCAGGT GTGGTCCCAG GACAGCTTTG GCCGCTGCCA GCTTGCAGGC 
TATGGATTTT GCCATGTGCC CAGTAGCCCG GGCACCCACC AGCTGGCCTG CCCCACGTGG 
CGGCCCCTGG GCAGTTGGCG AGAACAGTTG GCACGGGCTT TCGTGGGTGG TGGGCCGCAG 
CTGCTGCATG GGGACACCAT CTACAGTGGG GCCGACCGCT ATCGCCTGCA CACAGCTGCT 
GGTGGCACCG TGCACCTGGA GATCGGCCTG CTGCTCCGCA ACTTCGACCG CTACGGCGTG 
GAGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.
Nature communications711491(2016 May)
Boldt K,van Reeuwijk J,Lu Q,Koutroumpas K,Nguyen TM,Texier Y,van Beersum SE,Horn N,Willer JR,Mans DA,Dougherty G,Lamers IJ,Coene KL,Arts HH,Betts MJ,Beyer T,Bolat E,Gloeckner CJ,Haidari K,Hetterschijt L,Iaconis D,Jenkins D,Klose F,Knapp B,Latour B,Letteboer SJ,Marcelis CL,Mitic D,Morleo M,Oud MM,Riemersma M,Rix S,Terhal PA,Toedt G,van Dam TJ,de Vrieze E,Wissinger Y,Wu KM,Apic G,Beales PL,Blacque OE,Gibson TJ,Huynen MA,Katsanis N,Kremer H,Omran H,van Wijk E,Wolfrum U,Kepes F,Davis EE,Franco B,Giles RH,Ueffing M,Russell RB,Roepman R,

A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface.
Cell163(6)1484-99(2015 Dec)
Gupta GD,Coyaud ?,Gon?alves J,Mojarad BA,Liu Y,Wu Q,Gheiratmand L,Comartin D,Tkach JM,Cheung SW,Bashkurov M,Hasegan M,Knight JD,Lin ZY,Schueler M,Hildebrandt F,Moffat J,Gingras AC,Raught B,Pelletier L

Large-scale genetic study in East Asians identifies six new loci associated with colorectal cancer risk.
Nature genetics46(6)533-42(2014 Jun)
Zhang B,Jia WH,Matsuda K,Kweon SS,Matsuo K,Xiang YB,Shin A,Jee SH,Kim DH,Cai Q,Long J,Shi J,Wen W,Yang G,Zhang Y,Li C,Li B,Guo Y,Ren Z,Ji BT,Pan ZZ,Takahashi A,Shin MH,Matsuda F,Gao YT,Oh JH,Kim S,Ahn YO,,Chan AT,Chang-Claude J,Slattery ML,,Gruber SB,Schumacher FR,Stenzel SL,,Casey G,Kim HR,Jeong JY,Park JW,Li HL,Hosono S,Cho SH,Kubo M,Shu XO,Zeng YX,Zheng W

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B9D2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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