Gcn1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gcn1
Entrez Gene ID	231659
Full Name	GCN1 general control of amino-acid synthesis 1-like 1 (yeast)
Synonyms	4932409G22,AL022764,G431004K08Rik,GCN1L,Gcn1,mKIAA0219
General protein information	Preferred Names GCN1 general control of amino-acid synthesis 1-like 1 (yeast) Names eIF-2-alpha kinase activator GCN1 GCN1 eIF-2-alpha kinase activator homolog GCN1-like protein 1 general control of amino-acid synthesis 1-like protein 1 translational activator GCN1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 F

mRNA and Protein(s)

mRNA	Protein	Name
NM_172719.2	NP_766307.2	eIF-2-alpha kinase activator GCN1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GCN1L1	XP_534701.2
Danio rerio (zebrafish)	gcn1l1	XP_002667084.2
Caenorhabditis elegans (roundworm)	gcn-1	NP_497506.3
Oryza sativa (rice)	Os03g0721200	NP_001051102.1
Saccharomyces cerevisiae (baker's yeast)	GCN1	NP_011320.3
Schizosaccharomyces pombe (fission yeast)	SPAC18G6.05c	NP_593669.1
Magnaporthe oryzae (rice blast fungus)	MGG_04710	XP_003710832.1
Arabidopsis thaliana (thale cress)	ILA	NP_176659.6
Pan troglodytes (chimpanzee)	GCN1L1	XP_001159608.2
Mus musculus (house mouse)	Gcn1l1	NP_766307.2
Rattus norvegicus (Norway rat)	Gcn1l1	NP_001162135.1
Eremothecium gossypii	AGOS_AFR169W	NP_985716.2
Homo sapiens (human)	GCN1L1	NP_006827.1
Bos taurus (cattle)	GCN1L1	XP_002694568.1
Gallus gallus (chicken)	GCN1L1	XP_003642271.2
Drosophila melanogaster (fruit fly)	CG17514	NP_001015316.1
Neurospora crassa	NCU05803	XP_960063.1
Xenopus tropicalis (tropical clawed frog)	gcn1l1	NP_001096529.1
	GCN1L1	XP_001087027.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001700	XP_321391.4
Kluyveromyces lactis	KLLA0F12430g	XP_455642.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolutionarily conserved IMPACT impairs various stress responses that require GCN1 for activating the eIF2 kinase GCN2.
Cambiaghi TD, Pereira CM, Shanmugam R, Bolech M, Wek RC, Sattlegger E, Castilho BA
Biochemical and biophysical research communications443(2)592-7(2014 Jan)

IMPACT is a developmentally regulated protein in neurons that opposes the eukaryotic initiation factor 2? kinase GCN2 in the modulation of neurite outgrowth.
Roff? M, Hajj GN, Azevedo HF, Alves VS, Castilho BA
The Journal of biological chemistry288(15)10860-9(2013 Apr)

Evidence that Yih1 resides in a complex with ribosomes.
Waller T, Lee SJ, Sattlegger E
The FEBS journal279(10)1761-76(2012 May)

IMPACT, a protein preferentially expressed in the mouse brain, binds GCN1 and inhibits GCN2 activation.
Pereira CM, Sattlegger E, Jiang HY, Longo BM, Jaqueta CB, Hinnebusch AG, Wek RC, Mello LE, Castilho BA
The Journal of biological chemistry280(31)28316-23(2005 Aug)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study extends our understanding of the roles played by GCN1 in GCN2 activation induced by a variety of stress arrangements and suggests that IMPACT and YIH1 use similar mechanisms for regulating this eIF2alpha kinase.
Title: Evolutionarily conserved IMPACT impairs various stress responses that require GCN1 for activating the eIF2 kinase GCN2.

The following Gcn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gcn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu14229	NM_172719.2 Latest version!	Mus musculus GCN1 activator of EIF2AK4 (Gcn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gcn1 ( NM_172719.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766307.2 Mus musculus GCN1 activator of EIF2AK4 (Gcn1), mRNA.

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CloneID	OMu14229
Clone ID Related Accession (Same CDS sequence)	NM_172719.2
Accession Version	NM_172719.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Mus musculus(house mouse)
Product	eIF-2-alpha kinase activator GCN1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ104712.1, AK147257.1, CD350892.1, AK089324.1, AK077013.1 and BY582410.1. On or before Aug 20, 2006 this sequence version replaced XM_913913.2, XM_358358.4, XM_898535.2, XM_898519.2, XM_898531.2, XM_898526.2, XM_898512.2, XM_994281.1, XM_994266.1, XM_994253.1, XM_994207.1, XM_994186.1, XM_994172.1, XM_994149.1, NM_172719.1. ##Evidence-Data-START## Transcript exon combination :: AK090137.1, AK147257.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCGG ACACGCAGGT TTCCGAGACA CTGAAGCGCT TCGCTGTGAA GGTGACAACA 
GCCAGTGTGA AGGAGCGGAG AGAGATTCTC AGTGAACTGG GGCGATGCAT CGCCGGCAAA 
GATCTGCCAG AGGGAGCCGT GAAAGGGCTC TGCAAGTTGT TCTGCTTGAC ACTGCATCGA 
TATAGAGACG CAGCCTCTCG CAGAGCCCTG CAGGCAGCCA TCCAGCAGCT GGCCGAGGCC 
CAGCCAGAGG CCACCGCCAA GAACCTCCTG CACTCTCTCC AGTCTTCAGG GGTTGGCTCC 
AAAGCATGTG TTCCCAGCAA GAGTAGCGGC TCAGCTGCCT TGCTGGCTCT GACCTGGACC 
TGCCTCCTAG TGCGAATTGT CTTTCCCCTG AAGGCCAAGC GGCAAGGAGA CATCTGGAAC 
AAGCTGGTGG AGGTGCAGTG CCTGCTGCTG CTGGAGGTGC TGGGCGGCTC CCACAAGCAT 
GCTGTGGACG GTGCTGTGAA GAAGCTCACT AAGCTATGGA AGGAGAACCC TGGGCTGGTG 
GAGCAGTACT TCTCAGCCAT CCTTAGCCTG GAGCCCAGCC AAAACTATGC TGCCATGCTG 
GGGCTGCTGG TGCAGTTCTG CACGAACCAC AAAGAGATGG ACGCGGTCAG CCAGCATAAG 
AGCACCCTGC TGGAGTTCTA CGTGAAGAAC ATTCTGATGA GCAAAGCCAA GCCTCCCAAG 
TACTTGCTGG ACAACTGTGC CCCCTTGCTC CGGTTCATGT CCCACTCAGA GTTTAAGGAT 
CTGATTCTGC CCACCATACA GAAGTCCTTG CTGAGGAGCC CAGAGAATGT TATTGAAACT 
ATCTCCAGCC TGCTGGCTTC AGTGACACTC GATCTCAGCC AGTACGCCCT GGACATCGTG 
AAAGGCTTGG CAAATCAGCT GAAGTCCAAC AGCCCCCGCC TGATGGATGA AGCGGTCCTG 
GCGCTTCGCA ACCTGGCCCG CCAGTGCAGT GACTCCTCCG CCACTGAGGC CCTGACCAAG 
CACCTGTTTG CGATCCTTGG AGGCTCAGAA GGAAAGCTAA CCATCATAGC CCAGAAGATG 
AGTGTCCTTT CAGGGATTGG AAGCCTCAGT CATCACGTGG TATCGGGGCC GTCGGGTCAA 
GTCCTGAATG GCTGTGTGGC TGAGCTGTTC ATCCCCTTCC TGCAGCAGGA AGTGCACGAA 
GGTACCTTGG TACACGCCGT CTCCATCCTG GCGCTCTGGT GCAACCGGTT CACTACAGAA 
GTGCCCAAGA AGCTTACGGA CTGGTTCAAG AAAGTCTTCA GCCTTAAAAC GTCCACCTCT 
GCAGTGCGGC ACGCCTACCT GCAGTGCATG CTGGCCTCCT TCCGAGGTGA CACACTGTTG 
CAGGCCTTGG ACTTTCTGCC ATTGCTTATG CAGACAGTAG AGAAGGCGGC CTCCCAGGGC 
ACTCAGGTCC CCACAGTCAC CGAAGGGGTC GCCGCAGCCC TTCTGCTCTC CAAGCTGTCT 
GTGGCTGACG CCCAGGCCGA GGCCAAGCTG AGCGGCTTTT GGCAGCTGGT TGTGGATGAG 
AAGAGGCAGA CTTTCACGTC TGAGAAGTTC CTGCTCCTGG CTTCAGAGGA CGCCCTGTGC 
ACTGTGCTGC GTCTGACGGA GAGGCTTTTC CTTGACCACC CTCACAGGCT CACCAACAGC 
AAAGTCCAGC AGTACTATCG GGTCCTGGTG GCTGTGCTCC TGAGCCGGAC CTGGCATGTG 
CGCAGGCAGG CCCAGCAGAC AGTTCGGAAG CTGCTGTCCT CTCTTGGGGG TGTTAAGCTG 
GCCAATGGTC TCTTGGACGA ACTGAAGACT GTCCTCAACT CTCACAAGGT GCTTCCCTTG 
GAGGCTCTTG TGACGGACGC TGGGGAGGTA ACGGAGATGG GCAAGACCTA TGTGCCTCCT 
CGAGTTCTGC AGGAAGCTCT GTGTGTCATC TCTGGGGTGC CAGGGCTCAA GGGCGACATC 
CCCAGCACTG AGCAGCTGGC TCAGGAGATG CTAATCATCT CCCACCACCC GTCCTTAGTG 
GCAGTACAGT CTGGACTCTG GCCAGCACTT CTTACAAGGA TGAAGATTGA CCCTGACGCC 
TTCATCACCA GGCACCTGGA TCAGATCATC CCCAGGATCA CGACACAGAG TCCCCTGAAC 
CAGTCTTCCA TGAACGCCAT GGGCTCCCTT TCTGTCCTGT CTCCTGACCG CGTCCTCCCT 
CAGCTCATCA GCACCATCAC TGCCTCTGTG CAGAATCCTG CCCTGTGCCT TGTGACTCGG 
GAGGAGTTCT CCATCATGCA GACCCCTGCC GGGGAGCTGT TTGATAAATC CATCATCCAG 
AGTGCCCAGC AGGACAGCAT AAAAAAGGCC AACATGAAGC GCGAGAACAA GGCCTACTCT 
TTTAAGGAGC AGATCATCGA GATGGAGCTG AAGGAGGAAA TAAAGAAAAA GAAAGGCATA 
AAAGAGGAGG TACAGCTGAC CAGCAAGCAG AAGGAGATGC TGCAAGCCCA GATGGACAAG 
GAGGCACAAA TCCGGAGGCG GCTGCAGGAG CTGGACGGGG AACTAGAGGC GGCACTTGGA 
CTACTAGATG CCATCATGGC CAGGAACCCG TGTGGCCTGA TTCAGTACAT CCCTGTCCTG 
GTTGATGCCT TCCTGCCCTT GCTGAAGTCT CCCCTGGCTG CTCCCAGGGT CAAAGGCCCC 
TTTCTGTCCT TAGCTGCCTG TGTCATGCCC CCGAGACTCA AGACCTTGGG CACCTTGGTG 
AGTCATGTGA CCCTGCGCCT GCTGAAGCCA GAGTGTGCTC TGGATAAGTC CTGGTGCCAG 
GAGGAGCTGC CTGTGGCTGT CAGGAGGGCG GTGTCGCTGT TGCATACACA CACCATCCCC 
AGCAGGGTGG GCAAGGGCGA GCCAGATGCC GCCCCGCTGT CTGCACCGGC CTTCTCCTTG 
GTCTTCCCCA TGTTAAAGAT GGTGCTCACT GAGATGCCCT ACCACAGCGA GGAGGAGGAG 
GAGCAGATGG CCCAGATCCT TCAGATCCTC ACTGTCCATG CCCAGCTGAG GGCGTCCCCT 
GACACCCCAC CTGAGCGGGT GGACGAGAAT GGCCCGGAGT TGCTGCCTCG AGTGGCCATG 
CTGCGCCTGC TGACGTGGGT GATCGGGACG GGCTCCCCTC GCCTACAGGT TCTTGCCTCA 
GACACGCTGA CTGCCCTGTG TGCCAGCAGC AGCGGGGAGG ACGGCTGTGC CTTCGCAGAG 
CAGGAGGAGG TGGACGTGCT GCTTGCTGCC CTGCAGTCCC CATGTGCCAG CGTTCGCGAG 
ACAGCGCTCC GGGGCCTGAT GGAGCTCCGC CTAGTACTGC CATCACCCGA CACTGACGAG 
AAGAGTGGCC TGAGCCTGTT GCGGAGACTC TGGGTCATTA AGTTCGACAA GGAGGATGAG 
ATACGGAAGC TGGCCGAAAG GCTTTGGTCA ACCATGGGCC TCGATCTGCA GTCAGATCTC 
TGCTCTTTGC TGATCGATGA CGTGATCTAT CACGAGGCAG CCGTGAGGCA GGCCGGGGCT 
GAAGCCCTTT CCCAGGCGGT GGCACGGTAC CAGCGGCAGG CAGCGGAGGT TATGGGCAGG 
CTCATGGAGA TCTACCAGGA GAAGCTCTAT CGGCCACCCC CAGTGCTGGA TGCTTTGGGA 
CGAGTGATTT CAGAATCCCC TCCTGACCAG TGGGAAGCCA GGTGTGGCCT AGCCTTGGCC 
CTGAACAAGC TCTCGCAGTA CTTGGATAGC TCTCAGGTGA AGCCGCTTTT CCAGTTCTTT 
GTCCCGGATG CCCTGAATGA CCGGAACCCA GATGTGCGGA AGTGCATGTT AGATGCAGCG 
CTCGCGACGC TCAATGCCCA TGGGAAGGAG AATGTCAACT CACTGCTGCC AGTGTTTGAG 
GAGTTCCTGA AGGACGCACC CAATGATGCC AGCTACGACG CTGTGCGGCA GAGCGTGGTG 
GTCCTCATGG GGTCTCTGGC CAAACACCTG GACAAGAGTG ACCCCAAAGT TAAACCTATT 
GTTGCCAAAC TCATTGCCGC CCTCTCCACA CCCTCTCAGC AGGTCCAGGA GTCTGTGGCC 
AGCTGCCTGC CACCTCTCGT GCCTGCTGTC AAGGAGGATG CTGGGGGCAT GATCCAGCGG 
CTCATGCAGC AGCTGTTGGA GTCGGACAAG TATGCGGAGC GCAAAGGGGC GGCATACGGG 
CTGGCGGGCC TGGTCAAGGG CCTGGGCATC CTCTCCCTGA AGCAGCAGGA GATGATGGCC 
GCGCTGACTG ATGCTATCCA GGACAAGAAG AACTTCCGAA GGCGAGAGGG AGCGCTGTTT 
GCCTTCGAGA TGCTCTGTAC CATGCTGGGG AAGCTCTTCG AGCCTTATGT GGTCCACGTG 
CTGCCCCATC TGTTGCTGTG CTTTGGGGAT GGGAACCAGT ACGTGCGCGA GGCTGCAGAT 
GACTGTGCCA AAGCTGTGAT GAGCAATTTG AGTGCTCACG GTGTGAAGTT GGTGCTCCCG 
TCCTTGTTAG CTGCCCTGGA GGAGGAGTCC TGGAGGACCA AAGCTGGGTC CGTAGAGCTG 
CTCGGGGCAA TGGCGTACTG TGCACCTAAG CAGCTGTCGT CCTGCCTGCC CAACATTGTG 
CCCAAGCTCA CAGAAGTGCT GACAGACTCC CACGTCAAAG TGCAGAAGGC TGGACAGCAG 
GCCCTCAGGC AGATTGGCTC TGTCATCAGA AACCCGGAGA TTCTCGCCAT TGCCCCGGTC 
CTGCTGGATG CCCTGACAGA CCCATCACGG AAGACCCAGA AGTGCCTGCA GACCCTGCTG 
GACACCAAGT TTGTTCACTT CATTGATGCC CCGTCCCTGG CGCTCATCAT GCCCATTGTC 
CAGAGAGCCT TCCAGGACCG CTCCACAGAC ACACGAAAGA TGGCAGCGCA GATTATTGGC 
AACATGTACT CCCTGACAGA CCAGAAGGAC TTGGCTCCTT ACCTGCCCAG TGTGACACCT 
GGCCTGAAAG CTTCACTCTT GGACCCTGTC CCTGAGGTGC GCACCGTGTC TGCAAAGGCT 
CTTGGGGCCA TGGTGAAGGG CATGGGGGAG TCGTGCTTTG AAGATTTGCT GCCATGGCTG 
ATGGAGACTC TGACGTACGA GCAGAGCTCC GTGGATCGCT CAGGCGCTGC CCAAGGGTTG 
GCTGAGGTAA TGGCCGGCTT AGGAGTGGAG AAGTTGGAAA AACTGATGCC AGAGATCGTG 
GCCACAGCCA GCAAAGTGGA CATCGCACCC CACGTCCGCG ACGGCTACAT CATGATGTTC 
AACTACCTGC CCATCACCTT TGGGGACAAG TTTACCCCTT ATGTGGGGCC CATCATCCCT 
TGTATCCTCA AGGCTCTGGC TGATGAGAAC GAGTTCGTTC GTGACACCGC CCTGCGTGCC 
GGCCAGCGGG TCATCTCGAT GTACGCTGAG ACAGCTATTG CCCTGCTGCT GCCACAGCTA 
GAGCAAGGCC TCTTTGATGA CCTCTGGAGA ATACGGTTCA GCTCTGTTCA ACTTCTTGGG 
GACCTCCTGT TCCACATCTC TGGGGTCACA GGGAAGATGA CCACGGAAAC AGCGTCTGAG 
GATGATAACT TCGGAACTGC TCAGTCTAAC AAGGCAATCA TCACTGCCCT GGGGGTTGAC 
CGGCGGAACC GGGTCCTGGC GGGACTCTAC ATGGGCCGCT CAGACACCCA GCTGGTGGTG 
AGGCAGGCGT CGCTGCACGT GTGGAAGATT GTCGTCTCCA ACACTCCCCG CACCCTGCGA 
GAGATCCTGC CCACTCTCTT TGGCCTCCTA CTGGGTTTCC TGGCTAGCAC GTGTGCAGAT 
AAGAGAACGA TCGCAGCGAG GACGCTGGGC GACCTCGTCC GCAAGCTCGG GGAGAAGATC 
CTTCCTGAGA TCATCCCCAT CCTGGAAGAA GGACTAAGGT CGCAGAAGAG CGACGAGAGG 
CAGGGCGTGT GCATCGGGCT CAGCGAGATC ATGAAGTCCA CCAGCCGGGA CGCTGTGCTC 
TTCTTCTCTG AGTCCCTTGT GCCCACGGCA AGGAAGGCGC TGTGTGATCC CCTGGAGGAG 
GTCCGGGAGG CGGCAGCCAA GACCTTTGAG CAGCTGCACT CCACCATTGG CCACCAGGCT 
TTGGAGGACA TTCTCCCGTT CCTACTGAAG CAGCTGGACG ACGAGGAGGT GTCGGAATTT 
GCCCTGGACG GTCTGAAGCA GGTTATGGCC GTTAAGAGCC GTGTGGTGCT GCCCTATCTG 
GTGCCCAAGC TGACAACACC ACCCGTCAAT ACCCGGGTGC TGGCTTTCCT GTCCTCTGTG 
GCTGGCGATG CTCTGACCCG ACACCTTGGC GTGATCCTCC CAGCTGTCAT GTTAGCACTG 
AAGGAGAAGC TTGGGACTCC AGACGAGCAG CTGGAGATGG CCAACTGTCA GGCTGTGATC 
CTCTCAGTGG AGGATGACAC TGGGCACCGG ATCATCATCG AGGATCTGTT GGAAGCCACG 
CGCAGCCCTG AAGTGGGCAT GAGGCAGGCG GCGGCCATCA TCCTGAACAT GTACTGCTCC 
CGCTCCAAGG CCGACTACAG CAGCCACCTT CGCAGCCTGG TCTCGGGCTT GATCCGCCTC 
TTCAATGACT CCAGCCCTGT GGTTCTGGAG GAGAGCTGGG ACGCTCTCAA TGCCATCACC 
AAGAAGCTGG ACGCTGGTAA CCAGCTGGCC CTGATCGAAG AACTCCACAA GGAAATCCGC 
TTCATAGGCA ACGAATGCAA AGGGGAGCAC GTGCCAGGCT TCTGTCTTCC AAAGAGGGGA 
GTAACCTCCA TCCTTCCAGT GCTGCGGGAA GGGGTCCTCA CTGGCAGTCC TGAGCAGAAG 
GAAGAAGCAG CCAAGGGCTT GGGCTTGGTG ATCCGCCTGA CCTCAGCTGA TGCCCTGAGG 
CCCTCTGTGG TCAGCATCAC TGGCCCTCTG ATTCGCATCC TTGGGGACCG GTTCAACTGG 
ACTGTGAAGG CGGCTCTGCT GGAGACACTC AGCCTCTTGC TGGGCAAGGT TGGGATTGCC 
CTGAAGCCCT TCCTGCCCCA GCTTCAGACC ACCTTCACCA AAGCCCTACA GGACTCCAAT 
CGGGGCGTCC GGCTGAAGGC GGCCGATGCT CTGGGGAAGC TCATTTCCAT CCACGTCAAG 
GTGGACCCCC TCTTTACAGA GCTGCTCAAC GGCATCCGCG CGGTGGAGGA CCCGGGCATA 
CGAGACACCA TGTTGCAGGC CCTGAGGTTT GTGATTCAGG GTGCAGGCTC CAAAGTGGAT 
GCTGCCATTC GGAAAAACCT GGTCTCGCTC CTGCTGAGCA TGCTGGGCCA TGACGAGGAC 
AACACTCGGA TCTCCACAGC TGGGTGCCTT GGGGAACTGT GTGCCTTTCT GACTGACGAA 
GAGCTCAACA CTGTCCTTCA GCAGTGCCTG CTAGCTGACG TATCTGGCAT TGACTGGATG 
GTTCGGCACG GCCGGAGCTT GGCGCTGTCC GTGGCTGTGA ATGTGGCTCC CAGCAGACTG 
TGTGCAGGCA GATACAGCAA TGAGGTTCAG GACATGATCC TCAGCAACGC CGTGGCAGAC 
AGGATCCCCA TCGCCATGAG TGGGATTCGA GGCATGGGCT TCCTCATGAA GTATCACATC 
GAGACAGGAA GCGGGCAGCT GCCCCCCAGG CTCTCCAGCC TTCTCATCAA GTGTCTGCAG 
AACCCATGTA GTGACATCAG GCTGGTGGCC GAGAAGATGA TCTGGTGGGC AAACAAGGAG 
CCCCGGCCCC CCCTGGAGCC ACAGACCATC AAGCCCATCC TGAAGGCTCT TCTCGATAAC 
ACCAAGGACA AGAACACTGT CGTGCGGGCC TACAGCGACC AGGCCATCGT GAACCTGCTC 
AAGATGAGGC GGGGCGAGGA GCTGCTCCAG TCCCTCTCCA AGATCCTGGA CGTGGCCAGC 
CTGGAGGCTC TGAATGAATG CAGCCGGAGG TCGCTGAGGA AGCTGGCCTG CCAGGCAGAC 
TCGGTGGAGC AGGTGGACGA CACCATCCTG ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766307.2
CDS	27..8042
Translation	MAADTQVSETLKRFAVKVTTASVKERREILSELGRCIAGKDLPEGAVKGLCKLFCLTLHRYRDAASRRALQAAIQQLAEAQPEATAKNLLHSLQSSGVGSKACVPSKSSGSAALLALTWTCLLVRIVFPLKAKRQGDIWNKLVEVQCLLLLEVLGGSHKHAVDGAVKKLTKLWKENPGLVEQYFSAILSLEPSQNYAAMLGLLVQFCTNHKEMDAVSQHKSTLLEFYVKNILMSKAKPPKYLLDNCAPLLRFMSHSEFKDLILPTIQKSLLRSPENVIETISSLLASVTLDLSQYALDIVKGLANQLKSNSPRLMDEAVLALRNLARQCSDSSATEALTKHLFAILGGSEGKLTIIAQKMSVLSGIGSLSHHVVSGPSGQVLNGCVAELFIPFLQQEVHEGTLVHAVSILALWCNRFTTEVPKKLTDWFKKVFSLKTSTSAVRHAYLQCMLASFRGDTLLQALDFLPLLMQTVEKAASQGTQVPTVTEGVAAALLLSKLSVADAQAEAKLSGFWQLVVDEKRQTFTSEKFLLLASEDALCTVLRLTERLFLDHPHRLTNSKVQQYYRVLVAVLLSRTWHVRRQAQQTVRKLLSSLGGVKLANGLLDELKTVLNSHKVLPLEALVTDAGEVTEMGKTYVPPRVLQEALCVISGVPGLKGDIPSTEQLAQEMLIISHHPSLVAVQSGLWPALLTRMKIDPDAFITRHLDQIIPRITTQSPLNQSSMNAMGSLSVLSPDRVLPQLISTITASVQNPALCLVTREEFSIMQTPAGELFDKSIIQSAQQDSIKKANMKRENKAYSFKEQIIEMELKEEIKKKKGIKEEVQLTSKQKEMLQAQMDKEAQIRRRLQELDGELEAALGLLDAIMARNPCGLIQYIPVLVDAFLPLLKSPLAAPRVKGPFLSLAACVMPPRLKTLGTLVSHVTLRLLKPECALDKSWCQEELPVAVRRAVSLLHTHTIPSRVGKGEPDAAPLSAPAFSLVFPMLKMVLTEMPYHSEEEEEQMAQILQILTVHAQLRASPDTPPERVDENGPELLPRVAMLRLLTWVIGTGSPRLQVLASDTLTALCASSSGEDGCAFAEQEEVDVLLAALQSPCASVRETALRGLMELRLVLPSPDTDEKSGLSLLRRLWVIKFDKEDEIRKLAERLWSTMGLDLQSDLCSLLIDDVIYHEAAVRQAGAEALSQAVARYQRQAAEVMGRLMEIYQEKLYRPPPVLDALGRVISESPPDQWEARCGLALALNKLSQYLDSSQVKPLFQFFVPDALNDRNPDVRKCMLDAALATLNAHGKENVNSLLPVFEEFLKDAPNDASYDAVRQSVVVLMGSLAKHLDKSDPKVKPIVAKLIAALSTPSQQVQESVASCLPPLVPAVKEDAGGMIQRLMQQLLESDKYAERKGAAYGLAGLVKGLGILSLKQQEMMAALTDAIQDKKNFRRREGALFAFEMLCTMLGKLFEPYVVHVLPHLLLCFGDGNQYVREAADDCAKAVMSNLSAHGVKLVLPSLLAALEEESWRTKAGSVELLGAMAYCAPKQLSSCLPNIVPKLTEVLTDSHVKVQKAGQQALRQIGSVIRNPEILAIAPVLLDALTDPSRKTQKCLQTLLDTKFVHFIDAPSLALIMPIVQRAFQDRSTDTRKMAAQIIGNMYSLTDQKDLAPYLPSVTPGLKASLLDPVPEVRTVSAKALGAMVKGMGESCFEDLLPWLMETLTYEQSSVDRSGAAQGLAEVMAGLGVEKLEKLMPEIVATASKVDIAPHVRDGYIMMFNYLPITFGDKFTPYVGPIIPCILKALADENEFVRDTALRAGQRVISMYAETAIALLLPQLEQGLFDDLWRIRFSSVQLLGDLLFHISGVTGKMTTETASEDDNFGTAQSNKAIITALGVDRRNRVLAGLYMGRSDTQLVVRQASLHVWKIVVSNTPRTLREILPTLFGLLLGFLASTCADKRTIAARTLGDLVRKLGEKILPEIIPILEEGLRSQKSDERQGVCIGLSEIMKSTSRDAVLFFSESLVPTARKALCDPLEEVREAAAKTFEQLHSTIGHQALEDILPFLLKQLDDEEVSEFALDGLKQVMAVKSRVVLPYLVPKLTTPPVNTRVLAFLSSVAGDALTRHLGVILPAVMLALKEKLGTPDEQLEMANCQAVILSVEDDTGHRIIIEDLLEATRSPEVGMRQAAAIILNMYCSRSKADYSSHLRSLVSGLIRLFNDSSPVVLEESWDALNAITKKLDAGNQLALIEELHKEIRFIGNECKGEHVPGFCLPKRGVTSILPVLREGVLTGSPEQKEEAAKGLGLVIRLTSADALRPSVVSITGPLIRILGDRFNWTVKAALLETLSLLLGKVGIALKPFLPQLQTTFTKALQDSNRGVRLKAADALGKLISIHVKVDPLFTELLNGIRAVEDPGIRDTMLQALRFVIQGAGSKVDAAIRKNLVSLLLSMLGHDEDNTRISTAGCLGELCAFLTDEELNTVLQQCLLADVSGIDWMVRHGRSLALSVAVNVAPSRLCAGRYSNEVQDMILSNAVADRIPIAMSGIRGMGFLMKYHIETGSGQLPPRLSSLLIKCLQNPCSDIRLVAEKMIWWANKEPRPPLEPQTIKPILKALLDNTKDKNTVVRAYSDQAIVNLLKMRRGEELLQSLSKILDVASLEALNECSRRSLRKLACQADSVEQVDDTILT

Target ORF information:

RefSeq Version	NM_172719.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus GCN1 activator of EIF2AK4 (Gcn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172719.2

ORF Insert Sequence:

ATGGCGGCGG ACACGCAGGT TTCCGAGACA CTGAAGCGCT TCGCTGTGAA GGTGACAACA 
GCCAGTGTGA AGGAGCGGAG AGAGATTCTC AGTGAACTGG GGCGATGCAT CGCCGGCAAA 
GATCTGCCAG AGGGAGCCGT GAAAGGGCTC TGCAAGTTGT TCTGCTTGAC ACTGCATCGA 
TATAGAGACG CAGCCTCTCG CAGAGCCCTG CAGGCAGCCA TCCAGCAGCT GGCCGAGGCC 
CAGCCAGAGG CCACCGCCAA GAACCTCCTG CACTCTCTCC AGTCTTCAGG GGTTGGCTCC 
AAAGCATGTG TTCCCAGCAA GAGTAGCGGC TCAGCTGCCT TGCTGGCTCT GACCTGGACC 
TGCCTCCTAG TGCGAATTGT CTTTCCCCTG AAGGCCAAGC GGCAAGGAGA CATCTGGAAC 
AAGCTGGTGG AGGTGCAGTG CCTGCTGCTG CTGGAGGTGC TGGGCGGCTC CCACAAGCAT 
GCTGTGGACG GTGCTGTGAA GAAGCTCACT AAGCTATGGA AGGAGAACCC TGGGCTGGTG 
GAGCAGTACT TCTCAGCCAT CCTTAGCCTG GAGCCCAGCC AAAACTATGC TGCCATGCTG 
GGGCTGCTGG TGCAGTTCTG CACGAACCAC AAAGAGATGG ACGCGGTCAG CCAGCATAAG 
AGCACCCTGC TGGAGTTCTA CGTGAAGAAC ATTCTGATGA GCAAAGCCAA GCCTCCCAAG 
TACTTGCTGG ACAACTGTGC CCCCTTGCTC CGGTTCATGT CCCACTCAGA GTTTAAGGAT 
CTGATTCTGC CCACCATACA GAAGTCCTTG CTGAGGAGCC CAGAGAATGT TATTGAAACT 
ATCTCCAGCC TGCTGGCTTC AGTGACACTC GATCTCAGCC AGTACGCCCT GGACATCGTG 
AAAGGCTTGG CAAATCAGCT GAAGTCCAAC AGCCCCCGCC TGATGGATGA AGCGGTCCTG 
GCGCTTCGCA ACCTGGCCCG CCAGTGCAGT GACTCCTCCG CCACTGAGGC CCTGACCAAG 
CACCTGTTTG CGATCCTTGG AGGCTCAGAA GGAAAGCTAA CCATCATAGC CCAGAAGATG 
AGTGTCCTTT CAGGGATTGG AAGCCTCAGT CATCACGTGG TATCGGGGCC GTCGGGTCAA 
GTCCTGAATG GCTGTGTGGC TGAGCTGTTC ATCCCCTTCC TGCAGCAGGA AGTGCACGAA 
GGTACCTTGG TACACGCCGT CTCCATCCTG GCGCTCTGGT GCAACCGGTT CACTACAGAA 
GTGCCCAAGA AGCTTACGGA CTGGTTCAAG AAAGTCTTCA GCCTTAAAAC GTCCACCTCT 
GCAGTGCGGC ACGCCTACCT GCAGTGCATG CTGGCCTCCT TCCGAGGTGA CACACTGTTG 
CAGGCCTTGG ACTTTCTGCC ATTGCTTATG CAGACAGTAG AGAAGGCGGC CTCCCAGGGC 
ACTCAGGTCC CCACAGTCAC CGAAGGGGTC GCCGCAGCCC TTCTGCTCTC CAAGCTGTCT 
GTGGCTGACG CCCAGGCCGA GGCCAAGCTG AGCGGCTTTT GGCAGCTGGT TGTGGATGAG 
AAGAGGCAGA CTTTCACGTC TGAGAAGTTC CTGCTCCTGG CTTCAGAGGA CGCCCTGTGC 
ACTGTGCTGC GTCTGACGGA GAGGCTTTTC CTTGACCACC CTCACAGGCT CACCAACAGC 
AAAGTCCAGC AGTACTATCG GGTCCTGGTG GCTGTGCTCC TGAGCCGGAC CTGGCATGTG 
CGCAGGCAGG CCCAGCAGAC AGTTCGGAAG CTGCTGTCCT CTCTTGGGGG TGTTAAGCTG 
GCCAATGGTC TCTTGGACGA ACTGAAGACT GTCCTCAACT CTCACAAGGT GCTTCCCTTG 
GAGGCTCTTG TGACGGACGC TGGGGAGGTA ACGGAGATGG GCAAGACCTA TGTGCCTCCT 
CGAGTTCTGC AGGAAGCTCT GTGTGTCATC TCTGGGGTGC CAGGGCTCAA GGGCGACATC 
CCCAGCACTG AGCAGCTGGC TCAGGAGATG CTAATCATCT CCCACCACCC GTCCTTAGTG 
GCAGTACAGT CTGGACTCTG GCCAGCACTT CTTACAAGGA TGAAGATTGA CCCTGACGCC 
TTCATCACCA GGCACCTGGA TCAGATCATC CCCAGGATCA CGACACAGAG TCCCCTGAAC 
CAGTCTTCCA TGAACGCCAT GGGCTCCCTT TCTGTCCTGT CTCCTGACCG CGTCCTCCCT 
CAGCTCATCA GCACCATCAC TGCCTCTGTG CAGAATCCTG CCCTGTGCCT TGTGACTCGG 
GAGGAGTTCT CCATCATGCA GACCCCTGCC GGGGAGCTGT TTGATAAATC CATCATCCAG 
AGTGCCCAGC AGGACAGCAT AAAAAAGGCC AACATGAAGC GCGAGAACAA GGCCTACTCT 
TTTAAGGAGC AGATCATCGA GATGGAGCTG AAGGAGGAAA TAAAGAAAAA GAAAGGCATA 
AAAGAGGAGG TACAGCTGAC CAGCAAGCAG AAGGAGATGC TGCAAGCCCA GATGGACAAG 
GAGGCACAAA TCCGGAGGCG GCTGCAGGAG CTGGACGGGG AACTAGAGGC GGCACTTGGA 
CTACTAGATG CCATCATGGC CAGGAACCCG TGTGGCCTGA TTCAGTACAT CCCTGTCCTG 
GTTGATGCCT TCCTGCCCTT GCTGAAGTCT CCCCTGGCTG CTCCCAGGGT CAAAGGCCCC 
TTTCTGTCCT TAGCTGCCTG TGTCATGCCC CCGAGACTCA AGACCTTGGG CACCTTGGTG 
AGTCATGTGA CCCTGCGCCT GCTGAAGCCA GAGTGTGCTC TGGATAAGTC CTGGTGCCAG 
GAGGAGCTGC CTGTGGCTGT CAGGAGGGCG GTGTCGCTGT TGCATACACA CACCATCCCC 
AGCAGGGTGG GCAAGGGCGA GCCAGATGCC GCCCCGCTGT CTGCACCGGC CTTCTCCTTG 
GTCTTCCCCA TGTTAAAGAT GGTGCTCACT GAGATGCCCT ACCACAGCGA GGAGGAGGAG 
GAGCAGATGG CCCAGATCCT TCAGATCCTC ACTGTCCATG CCCAGCTGAG GGCGTCCCCT 
GACACCCCAC CTGAGCGGGT GGACGAGAAT GGCCCGGAGT TGCTGCCTCG AGTGGCCATG 
CTGCGCCTGC TGACGTGGGT GATCGGGACG GGCTCCCCTC GCCTACAGGT TCTTGCCTCA 
GACACGCTGA CTGCCCTGTG TGCCAGCAGC AGCGGGGAGG ACGGCTGTGC CTTCGCAGAG 
CAGGAGGAGG TGGACGTGCT GCTTGCTGCC CTGCAGTCCC CATGTGCCAG CGTTCGCGAG 
ACAGCGCTCC GGGGCCTGAT GGAGCTCCGC CTAGTACTGC CATCACCCGA CACTGACGAG 
AAGAGTGGCC TGAGCCTGTT GCGGAGACTC TGGGTCATTA AGTTCGACAA GGAGGATGAG 
ATACGGAAGC TGGCCGAAAG GCTTTGGTCA ACCATGGGCC TCGATCTGCA GTCAGATCTC 
TGCTCTTTGC TGATCGATGA CGTGATCTAT CACGAGGCAG CCGTGAGGCA GGCCGGGGCT 
GAAGCCCTTT CCCAGGCGGT GGCACGGTAC CAGCGGCAGG CAGCGGAGGT TATGGGCAGG 
CTCATGGAGA TCTACCAGGA GAAGCTCTAT CGGCCACCCC CAGTGCTGGA TGCTTTGGGA 
CGAGTGATTT CAGAATCCCC TCCTGACCAG TGGGAAGCCA GGTGTGGCCT AGCCTTGGCC 
CTGAACAAGC TCTCGCAGTA CTTGGATAGC TCTCAGGTGA AGCCGCTTTT CCAGTTCTTT 
GTCCCGGATG CCCTGAATGA CCGGAACCCA GATGTGCGGA AGTGCATGTT AGATGCAGCG 
CTCGCGACGC TCAATGCCCA TGGGAAGGAG AATGTCAACT CACTGCTGCC AGTGTTTGAG 
GAGTTCCTGA AGGACGCACC CAATGATGCC AGCTACGACG CTGTGCGGCA GAGCGTGGTG 
GTCCTCATGG GGTCTCTGGC CAAACACCTG GACAAGAGTG ACCCCAAAGT TAAACCTATT 
GTTGCCAAAC TCATTGCCGC CCTCTCCACA CCCTCTCAGC AGGTCCAGGA GTCTGTGGCC 
AGCTGCCTGC CACCTCTCGT GCCTGCTGTC AAGGAGGATG CTGGGGGCAT GATCCAGCGG 
CTCATGCAGC AGCTGTTGGA GTCGGACAAG TATGCGGAGC GCAAAGGGGC GGCATACGGG 
CTGGCGGGCC TGGTCAAGGG CCTGGGCATC CTCTCCCTGA AGCAGCAGGA GATGATGGCC 
GCGCTGACTG ATGCTATCCA GGACAAGAAG AACTTCCGAA GGCGAGAGGG AGCGCTGTTT 
GCCTTCGAGA TGCTCTGTAC CATGCTGGGG AAGCTCTTCG AGCCTTATGT GGTCCACGTG 
CTGCCCCATC TGTTGCTGTG CTTTGGGGAT GGGAACCAGT ACGTGCGCGA GGCTGCAGAT 
GACTGTGCCA AAGCTGTGAT GAGCAATTTG AGTGCTCACG GTGTGAAGTT GGTGCTCCCG 
TCCTTGTTAG CTGCCCTGGA GGAGGAGTCC TGGAGGACCA AAGCTGGGTC CGTAGAGCTG 
CTCGGGGCAA TGGCGTACTG TGCACCTAAG CAGCTGTCGT CCTGCCTGCC CAACATTGTG 
CCCAAGCTCA CAGAAGTGCT GACAGACTCC CACGTCAAAG TGCAGAAGGC TGGACAGCAG 
GCCCTCAGGC AGATTGGCTC TGTCATCAGA AACCCGGAGA TTCTCGCCAT TGCCCCGGTC 
CTGCTGGATG CCCTGACAGA CCCATCACGG AAGACCCAGA AGTGCCTGCA GACCCTGCTG 
GACACCAAGT TTGTTCACTT CATTGATGCC CCGTCCCTGG CGCTCATCAT GCCCATTGTC 
CAGAGAGCCT TCCAGGACCG CTCCACAGAC ACACGAAAGA TGGCAGCGCA GATTATTGGC 
AACATGTACT CCCTGACAGA CCAGAAGGAC TTGGCTCCTT ACCTGCCCAG TGTGACACCT 
GGCCTGAAAG CTTCACTCTT GGACCCTGTC CCTGAGGTGC GCACCGTGTC TGCAAAGGCT 
CTTGGGGCCA TGGTGAAGGG CATGGGGGAG TCGTGCTTTG AAGATTTGCT GCCATGGCTG 
ATGGAGACTC TGACGTACGA GCAGAGCTCC GTGGATCGCT CAGGCGCTGC CCAAGGGTTG 
GCTGAGGTAA TGGCCGGCTT AGGAGTGGAG AAGTTGGAAA AACTGATGCC AGAGATCGTG 
GCCACAGCCA GCAAAGTGGA CATCGCACCC CACGTCCGCG ACGGCTACAT CATGATGTTC 
AACTACCTGC CCATCACCTT TGGGGACAAG TTTACCCCTT ATGTGGGGCC CATCATCCCT 
TGTATCCTCA AGGCTCTGGC TGATGAGAAC GAGTTCGTTC GTGACACCGC CCTGCGTGCC 
GGCCAGCGGG TCATCTCGAT GTACGCTGAG ACAGCTATTG CCCTGCTGCT GCCACAGCTA 
GAGCAAGGCC TCTTTGATGA CCTCTGGAGA ATACGGTTCA GCTCTGTTCA ACTTCTTGGG 
GACCTCCTGT TCCACATCTC TGGGGTCACA GGGAAGATGA CCACGGAAAC AGCGTCTGAG 
GATGATAACT TCGGAACTGC TCAGTCTAAC AAGGCAATCA TCACTGCCCT GGGGGTTGAC 
CGGCGGAACC GGGTCCTGGC GGGACTCTAC ATGGGCCGCT CAGACACCCA GCTGGTGGTG 
AGGCAGGCGT CGCTGCACGT GTGGAAGATT GTCGTCTCCA ACACTCCCCG CACCCTGCGA 
GAGATCCTGC CCACTCTCTT TGGCCTCCTA CTGGGTTTCC TGGCTAGCAC GTGTGCAGAT 
AAGAGAACGA TCGCAGCGAG GACGCTGGGC GACCTCGTCC GCAAGCTCGG GGAGAAGATC 
CTTCCTGAGA TCATCCCCAT CCTGGAAGAA GGACTAAGGT CGCAGAAGAG CGACGAGAGG 
CAGGGCGTGT GCATCGGGCT CAGCGAGATC ATGAAGTCCA CCAGCCGGGA CGCTGTGCTC 
TTCTTCTCTG AGTCCCTTGT GCCCACGGCA AGGAAGGCGC TGTGTGATCC CCTGGAGGAG 
GTCCGGGAGG CGGCAGCCAA GACCTTTGAG CAGCTGCACT CCACCATTGG CCACCAGGCT 
TTGGAGGACA TTCTCCCGTT CCTACTGAAG CAGCTGGACG ACGAGGAGGT GTCGGAATTT 
GCCCTGGACG GTCTGAAGCA GGTTATGGCC GTTAAGAGCC GTGTGGTGCT GCCCTATCTG 
GTGCCCAAGC TGACAACACC ACCCGTCAAT ACCCGGGTGC TGGCTTTCCT GTCCTCTGTG 
GCTGGCGATG CTCTGACCCG ACACCTTGGC GTGATCCTCC CAGCTGTCAT GTTAGCACTG 
AAGGAGAAGC TTGGGACTCC AGACGAGCAG CTGGAGATGG CCAACTGTCA GGCTGTGATC 
CTCTCAGTGG AGGATGACAC TGGGCACCGG ATCATCATCG AGGATCTGTT GGAAGCCACG 
CGCAGCCCTG AAGTGGGCAT GAGGCAGGCG GCGGCCATCA TCCTGAACAT GTACTGCTCC 
CGCTCCAAGG CCGACTACAG CAGCCACCTT CGCAGCCTGG TCTCGGGCTT GATCCGCCTC 
TTCAATGACT CCAGCCCTGT GGTTCTGGAG GAGAGCTGGG ACGCTCTCAA TGCCATCACC 
AAGAAGCTGG ACGCTGGTAA CCAGCTGGCC CTGATCGAAG AACTCCACAA GGAAATCCGC 
TTCATAGGCA ACGAATGCAA AGGGGAGCAC GTGCCAGGCT TCTGTCTTCC AAAGAGGGGA 
GTAACCTCCA TCCTTCCAGT GCTGCGGGAA GGGGTCCTCA CTGGCAGTCC TGAGCAGAAG 
GAAGAAGCAG CCAAGGGCTT GGGCTTGGTG ATCCGCCTGA CCTCAGCTGA TGCCCTGAGG 
CCCTCTGTGG TCAGCATCAC TGGCCCTCTG ATTCGCATCC TTGGGGACCG GTTCAACTGG 
ACTGTGAAGG CGGCTCTGCT GGAGACACTC AGCCTCTTGC TGGGCAAGGT TGGGATTGCC 
CTGAAGCCCT TCCTGCCCCA GCTTCAGACC ACCTTCACCA AAGCCCTACA GGACTCCAAT 
CGGGGCGTCC GGCTGAAGGC GGCCGATGCT CTGGGGAAGC TCATTTCCAT CCACGTCAAG 
GTGGACCCCC TCTTTACAGA GCTGCTCAAC GGCATCCGCG CGGTGGAGGA CCCGGGCATA 
CGAGACACCA TGTTGCAGGC CCTGAGGTTT GTGATTCAGG GTGCAGGCTC CAAAGTGGAT 
GCTGCCATTC GGAAAAACCT GGTCTCGCTC CTGCTGAGCA TGCTGGGCCA TGACGAGGAC 
AACACTCGGA TCTCCACAGC TGGGTGCCTT GGGGAACTGT GTGCCTTTCT GACTGACGAA 
GAGCTCAACA CTGTCCTTCA GCAGTGCCTG CTAGCTGACG TATCTGGCAT TGACTGGATG 
GTTCGGCACG GCCGGAGCTT GGCGCTGTCC GTGGCTGTGA ATGTGGCTCC CAGCAGACTG 
TGTGCAGGCA GATACAGCAA TGAGGTTCAG GACATGATCC TCAGCAACGC CGTGGCAGAC 
AGGATCCCCA TCGCCATGAG TGGGATTCGA GGCATGGGCT TCCTCATGAA GTATCACATC 
GAGACAGGAA GCGGGCAGCT GCCCCCCAGG CTCTCCAGCC TTCTCATCAA GTGTCTGCAG 
AACCCATGTA GTGACATCAG GCTGGTGGCC GAGAAGATGA TCTGGTGGGC AAACAAGGAG 
CCCCGGCCCC CCCTGGAGCC ACAGACCATC AAGCCCATCC TGAAGGCTCT TCTCGATAAC 
ACCAAGGACA AGAACACTGT CGTGCGGGCC TACAGCGACC AGGCCATCGT GAACCTGCTC 
AAGATGAGGC GGGGCGAGGA GCTGCTCCAG TCCCTCTCCA AGATCCTGGA CGTGGCCAGC 
CTGGAGGCTC TGAATGAATG CAGCCGGAGG TCGCTGAGGA AGCTGGCCTG CCAGGCAGAC 
TCGGTGGAGC AGGTGGACGA CACCATCCTG ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolutionarily conserved IMPACT impairs various stress responses that require GCN1 for activating the eIF2 kinase GCN2.
Biochemical and biophysical research communications443(2)592-7(2014 Jan)
Cambiaghi TD,Pereira CM,Shanmugam R,Bolech M,Wek RC,Sattlegger E,Castilho BA

IMPACT is a developmentally regulated protein in neurons that opposes the eukaryotic initiation factor 2? kinase GCN2 in the modulation of neurite outgrowth.
The Journal of biological chemistry288(15)10860-9(2013 Apr)
Roff? M,Hajj GN,Azevedo HF,Alves VS,Castilho BA

Evidence that Yih1 resides in a complex with ribosomes.
The FEBS journal279(10)1761-76(2012 May)
Waller T,Lee SJ,Sattlegger E

IMPACT, a protein preferentially expressed in the mouse brain, binds GCN1 and inhibits GCN2 activation.
The Journal of biological chemistry280(31)28316-23(2005 Aug)
Pereira CM,Sattlegger E,Jiang HY,Longo BM,Jaqueta CB,Hinnebusch AG,Wek RC,Mello LE,Castilho BA

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Gcn1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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