GCN1 cDNA ORF clone, Bos taurus(cattle)

The following GCN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa224655 XM_024977776.1
Latest version!
Bos taurus GCN1, eIF2 alpha kinase activator homolog (GCN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa09078 XM_616264.8
Latest version!
Bos taurus GCN1, eIF2 alpha kinase activator homolog (GCN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa09078 XM_002694522.4
Latest version!
Bos taurus GCN1, eIF2 alpha kinase activator homolog (GCN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa224654 XM_002694522.5
Latest version!
Bos taurus GCN1, eIF2 alpha kinase activator homolog (GCN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa224655
    Clone ID Related Accession (Same CDS sequence) XM_024977776.1
    Accession Version XM_024977776.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eIF-2-alpha kinase activator GCN1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGGCGGCGG ACACGCAGGT TTCTGAGACA CTAAAGCGTT TTGCAGGGAA GGTGACGACA 
    GCCAGTGTGA AGGAACGGAG AGAAATCCTC AGTGAGCTGG GGAAATGTGT TGCCGGAAAA
    GATCTGCCGG AGGGAGCTGT GAAAGGCCTC TGCAAACTCT TCTGCTTGAC CCTGCATCGA
    TACAGGGATG CAGCCTCACG CCGGGCCCTG CAGGCAGCCA TCCAGCAGCT GGCTGAGGCC
    CAGCCTGAAG CCACTGCCAA GAACCTTCTG CACTCTCTGC AGTCCTCGGG CATCGGCTCC
    AAAGCGGGTG TCCCCAGTAA GAGCAGCGGC TCGGCTGCCT TGCTGGCCCT GTCCTGGACT
    GGCCTACTGG TGCGCATCGT CTTTCCCTCG AGAGCCAAGC GGCAAGGAGA CATCTGGAAC
    AAACTGGTGG AGGTGCAGTG CCTGCTCCTG CTGGAGGTGC TGGGTGGCTC CCACAAGCAC
    GCCGTGGACA GTGCCGTGAA GAAGCTCACC AAGCTGTGGA GAGAGAACCC CGGGCTGGTG
    GAGCAGTACT TGTCAGCCAT TCTCAGCCTG GAGCCCAACC AGAGCTACGC AGGCATGCTG
    GGGCTGCTGG TGCAGTTCTG CACGAGCCAC AAGGAGATGG ACGTGGCCAA CCAGCACAAG
    AGTGCCCTGC TGGACTTTTA CGTGAAGAAC ATCCTGATGA GCAAAGTGAA GCCTCAGAAG
    TATCTGTTGG ATAACTGTGC CCCTTTGCTC CGGTACGTGT CCCACTCGGA GTTTAAGGAT
    CTGGTCCTGC CCACCATACA GAAGTCCTTA CTGAGGAGTC CAGAGAATGT CATTGAAACT
    ATCTCCAGTC TGCTGGCATC CGTGACTCTG GATCTCAGCC AGTATGCCCT GGACATTGGG
    AAAGGACTGG CTGGTCAGCT GAAGTCCAAC AGCCCTCGCT TGATGGATGA AGCCGTGCTG
    GCGCTGCGGA ACCTGGCCCG CCAGTGCAGT GACTCCTCGG CGATGGAAGC CCTGGCCAGG
    CACCTGTTCG CTATCCTCGG AGGCTCGGAG GGGAAGCTAA CTATTGTAGC CCAGAAGATG
    AGCGTTCTCT CAGGGATCGG GAGTGTCAGT CATCACGTGG TGTCGGGGCC TTCTAGTCAA
    GTCCTGAGCG GGACCGTGGC TGAGCTCTTC ATCCCGTTCC TCCAGCAAGA AGTTCATGAA
    GGCACCTTGG TGCACGCTGT CTCTGTCTTG GCTCTCTGGT GTCACCGCTT CACCACGGAA
    GTACCCAAGA AGCTCACCGA ATGGTTCAAG AAAGCCTTCA GCCTTAAAAC CTCCACCTCT
    GCCGTGAGGC ACGCCTACCT GCAGTGCATG TTGGTCTGCT TTCGAGGCGA CACACTGCTG
    CAGGCCCTGG ACTTGCTGCC CTTGCTCATC CAGACAGTGG AGAAGGCGGC CTCCCAAGGC
    ACTCAGGTTC CCGTTGTCAC CGAAGGGGTC GCCGCTGCCT TGCTGCTCAC GAAGCTCTCA
    ATGGCCGACT CGCAGGCTGA GGCCAAACTG AGTGGTTTCT GGCAGCTCAT TTTGGATGAG
    AAGAAGCAGG TTTTCACTTC CGAGAAATTC CTCCTCATGG CTTCAGAGGA TGCCCTGTGT
    ACCGTGCTGC ACCTGACAGA GAGACTGTTT CTTGACCATC TGCACCGACT CACAGGCAAC
    AAAGTTCAGC AGTACCACCG GGCCCTGGCG GCCGTGCTCC TGAGCCGCCC CTGGCACGTG
    CGCAGGCAGG CCCAGCAGAC GGCCCGGAAG CTGCTGTCCT CTCTCGGGGG CTTCAAGCTG
    GCTTACGGAC TGCTGGAGGA GCTGAAGACC GTCCTCAGTT CTCACAAGGT GCTGCCCTTC
    GAGGCTCTGG TGACTGACAC TGGGGAGGTG ACTGAGGCGG GCAAGGCCTA CGTGCCCCCG
    CGGGTCCTGC AGGAGGCTCT CTGTGTCATC TCCGGGGTGC CAGGGCTGGA GGGTGGCATC
    ACCGGCATGG AACAGCTGGC ACAGGAGATG CTGATCATCT CTCACCACCC ATCCTTAGTG
    GCTGTGCAGT CTGGCCTCTG GCCAGCACTT CTCGCCAGGA TGAAGATCGA TCCGGAAGCC
    TTCATTAGTA GGCACTTGGA CCAGATCATC CCCAGGATCA CCACCCAGAA CCCCTTGAAC
    CAGTCTTCCA TGAACGCCAT GGGCTCCCTC TCGATCCTGT CCCCAGACCG GGTCCTCCCG
    CAGCTCATCA GCACCATCAC CACCTCCGTG CAGAACCCAG CACTGTGCCA GGTGACGCGG
    GAGGAGTTTG CTATCATGCA GACCCCTGCA GGGGAGTTGT ACGACAAGTC CATCATTCAG
    AGTGCCCAGC AGGACAGCAT AAAAAAGGCC AACATGAAGC GAGAGAACAA AGCTTACTCC
    TTCAAGGAGC AGATCATCGA GTTGGAGCTG AAGGAGGAGA TAAAGAAGAA GAAAGGCATA
    AAAGAGGAGG TGCAGCTGAC CAGCAAACAG AAGGAGATGC TGCAGGCCCA GCTGGACAAG
    GAGGCGCAGA TCCGGAGGCG GCTGCAGGAG CTGGACAGTG AGCTGGAGGC GGCTCTGGGG
    CTGCTGGACA CCATCCTGGC CAAGAACCCG TCCGGCCTGA CCCAGTACAT CCCGGTCCTG
    GTCGACTCTT TCCTGCCCTT GCTGAAGTCT CCCCTGGCTG CTCCCAGGAT CAAGACCCCT
    TTCCTGTCCT TGGCTGCCTG TGTCATGCCC CCGAGGCTCA AGGCTTTGGG CACTTTGGTG
    AGCCATGTGA CTCTGCGCCT GCTGAAGCCG GAATGTGCCC TGGACGAGTC ATGGTGCCAG
    GAAGAGCTGT CGGTGGCCGT GAAGAGAGCA GTGACCCTGC TGCACAGTCA CACCATCACC
    AGCAGGGCAG GCAAGGGCGA GCCAGACGCT GCGCCCCTGT CCGCGCCAGC CTTCTCCTTG
    GTTTTCCCGT TTCTGAAGAT GGTGCTGACT GAGATGCCCC ACCACAGTGA GGAGGAGGAG
    GAAAGAATGG CCCAGATTCT TCAGATCCTC ACAGTCCACG CCCAGCTGCG GGCCTCCCCA
    AGCAACCCAC CTGGGCGTGT GGACGAGAAC GGCCCGGAGT TGCTGCCTCG CGTGGCCATG
    CTGCGCCTTC TGACCTGGGT GATTGGGACG GGCTCTCCCC GACTTCAGGT TCTGGCTTCA
    GAGGCCCTGA CCACCCTGTG TGCCAGCAGC AGTGGCGAGG ATGGCTGTGC CTTTGCAGAG
    CAGGAGGAGG TGGACGTGCT GCTCTGCGCC CTGCTGTCCC CCTGTGCCAA CGTGCGGGAC
    ACTGTACTCC GGGGCCTGTT GGAACTCCAC ATGGTGTTGC CGGCGCCTGA CACCGATGAG
    AAGAATGGCC TGAACCTTCT CCGCAGGCTC TGGGTGGTTA AGTTCGACAA GGAGGAAGAG
    ATCCGAAAGT TGGCCGAGAG GCTCTGGTCG ACCATGGGCC TAGATCTGCA GCCAGACCTC
    TGCTCCTTGC TCATCGACGA CGTCATCTAT CACGAGGCAG CTGTGAGGCA GGCCGGGGCG
    GAAGCCCTCT CCCAGGCGGT GGCGCGTTAC CAGCGGCAGG CGGCTGAGGT CATGGGCAGG
    CTCATGGAGA TCTACCAGGA GAAGCTCTAT CGGCCGCCCC CGGTGCTGGA TGCTCTGGGA
    CGAGTTATCT CCGAATCTCC TCCAGACCAG TGGGAAGCCA GGTGTGGCTT GGCTTTGGCC
    CTGAACAAGC TCTCCCAGTG TTTGGACAGC TCTCAGGTGA AGCCACTCTT TCAATTTTTT
    GTCCCCGACG CCCTCAACGA CCGAAACCCA GATGTCCGGA AGTGCATGTT GGATGCAGCT
    CTTGCGACAC TCAACACACA TGGAAAGGAG AACGTCAACT CGCTGCTGCC GGTGTTCGAG
    GAGTTCCTGA AGAACGCGCC GAACGATGCC AGCTACGATG CCGTGCGGCA GAGCGTGGTG
    GTCCTCATGG GCTCTCTAGC CAAGCACCTG GACAAGAGTG ACCCCAAAGT GAAGCCGATT
    GTCGCCAAGC TCATTGCTGC CCTCGCCACC CCCTCCCAGC AGGTCCAGGA ATCCGTGGCC
    AACTGCTTGC CACCCCTCGT CCCTGCCATA AAGGAAGACG CGGGGGGTAT GATCCAGAGG
    CTGATGCAGC AGCTGCTGGA GTCAGACAAG TACGCCGAGC GCAAAGGGGC TGCGTACGGG
    CTGGCGGGCC TGGTGAAGGG CCTGGGCATC CTGTCGCTGA AGCAGCAGGA GATGATGGCC
    GCGCTGACCG ATGCCATTCA GGACAAGAAG AACTTCCGCC GCCGGGAGGG AGCCCTCTTT
    GCCTTCGAGA TGCTCTGCAC CATGCTGGGG AAGCTCTTTG AGCCGTATGT GGTCCACGTG
    CTGCCCCATC TGCTGCTCTG CTTTGGGGAC GGGAACCAGT ATGTGCGGGA GGCTGCAGAT
    GACTGTGCCA AGGCTGTGAT GAGCAACCTG AGTGCTCATG GGGTGAAGCT GGTGCTCCCC
    TCCTTGTTGG CTGCCCTGGA GGAGGAATCC TGGAGAACCA AAGCCGGGTC GGTGGAGTTG
    CTCGGGGCCA TGGCCTACTG TGCTCCCAAG CAGCTGTCGT CCTGCCTACC CAACATTGTG
    CCCAAGCTCA CAGAGGTGCT CACCGACTCC CACGTCAAAG TGCAGAAGGC TGGGCAGCAG
    GCGCTCAGGC AGATCGGCTC TGTCATCAGG AACCCAGAGA TCCTGGCCAT CGCCCCGGTA
    CTGCTGGACG CCCTGACAGA CCCCTCCCGG AAGACCCAGA AGTGCTTGCA GACCTTGCTG
    GACACCAAGT TTGTCCACTT CATCGATGCA CCGTCCCTGG CCCTCATCAT GCCCATCGTC
    CAGAGAGCCT TCCAGGACCG TTCCACGGAC ACACGGAAGA TGGCCGCCCA GATCATTGGC
    AACATGTACT CCCTGACAGA CCAGAAGGAC TTGGCTCCTT ACCTGCCAAG CGTGACGCCT
    GGTCTGAAAG CCTCTCTCCT GGATCCTGTG CCTGAGGTAC GGACAGTGTC TGCAAAGGCT
    CTTGGGGCCA TGGTCAAGGG CATGGGGGAG TCATGCTTTG AGGACTTGCT GCCGTGGCTG
    ATGGAGACAC TGACCTACGA GCAGAGCTCT GTGGATCGCT CAGGCGCCGC GCAGGGGTTG
    GCTGAGGTCA TGGCCGGCTT GGGGGTGGAG AAGTTGGAGA AGCTGATGCC AGAAATTGTG
    GCTACAGCCA GCAAAGTGGA CATTGCCCCC CACGTCCGAG ACGGCTACAT CATGATGTTC
    AACTACCTGC CCATCACCTT CGGGGACAAA TTCACTCCTT ATGTGGGGCC CATCATCCCC
    TGTATCCTCA AAGCTCTTGC TGATGAGAAC GAGTTTGTGC GTGATACTGC CCTGCGTGCA
    GGCCAGCGGG TCATCTCCAT GTATGCTGAG ACGGCCATTG CCCTGCTGCT GCCCCAGCTG
    GAGCAAGGCC TCTTTGATGA CCTCTGGAGA ATAAGGTTCA GCTCTGTTCA GCTCCTTGGC
    GATCTCCTGT TCCACATCTC AGGAGTCACT GGGAAGATGA CCACAGAAAC TGCCTCAGAG
    GATGACAACT TTGGAACCGC GCAGTCCAAC AAGGCGATTA TCACCGCCCT GGGGGTAGAT
    CGGCGGAACC GGGTGTTGGC GGGGCTGTAC ATGGGCCGCT CGGACACCCA GCTGGTGGTG
    CGGCAGGCCT CCCTGCACGT CTGGAAGATT GTGGTCTCTA ACACCCCCCG CACCTTGCGT
    GAGATCCTTC CCACCCTTTT TGGGCTCCTG CTGGGTTTCC TGGCCAGCAC ATGCGCGGAT
    AAGAGAACAA TCGCAGCCAG GACGCTGGGA GATCTCGTCC GGAAGTTAGG GGAGAAAATC
    CTGCCGGAGA TCATCCCCAT CCTGGAGGAA GGCCTGAGAT CTCCCAAGAG TGACGAGAGG
    CAGGGCGTGT GCATCGGGCT GAGCGAGATC ATGAAGTCCA CCAGCCGGGA TGCGGTGCTG
    TATTTCTCCG AGTCCCTCGT GCCCACGGCG AGGAAGGCTT TGTGTGACCC GCTGGAGGAG
    GTCAGAGAGG CGGCGGCCAA GACGTTTGAG CAGCTGCACT CCACCATCGG CCACCAGGCC
    CTGGAGGACA TCCTCCCGTT TTTACTGAAG CAGCTGGATG ACGAGGAGGT GTCCGAGTTT
    GCCTTGGATG GTCTGAAGCA GGTGATGGCC ATCAAGAGTC GTGTGGTGCT GCCCTACCTC
    GTGCCCAAGG AGATGGCCAA CTGTCAGGCC GTGATCCTGT CCGTGGAGGA CGACACCGGG
    CACCGGATCA TCATCGAGGA CCTGTTGGAA GCCACCCGCA GCCCTGAGGT GGGCATGAGG
    CAGGCCGCGG CCATCATCCT CAACATCTAC TGCTCACGCT CCAAGGCGGA CTACACTAGC
    CACCTGCGGA GCCTGGTCTC AGGCCTGATC CGCCTCTTCA ACGACTCCAG CCCCGTGGTT
    CTGGAGGAGA GCTGGGACGC CCTGAATGCC ATCACCAAGA AACTGGATGC AGGCAACCAG
    CTGGCCCTCA TTGAGGAACT GCACAAGGAA ATCCGGTTCA TAGGAAATGA GAGCAGAGGC
    GAGCACGTGC CAGGCTTCTG CCTCCCGAAG AAGGGGGTAA CGTCCATCCT TCCGGTGCTG
    CGGGAGGGGG TCCTGACTGG CAGCCCGGAA CAGAAGGAGG AAGCTGCCAA AGCGTTAGGC
    TTGGTGATCC GCCTGACCTC GGCGGACGCC CTGCGGCCAT CCGTGGTCAA CATCACTGGC
    CCTCTGATTC GCATCCTGGG GGACCGGTTC AGCTGGAACG TGAAGGCGGC TCTGCTCGAG
    ACACTCAGCC TCTTGCTGGC CAAGGTCGGG ATCGCCCTGA AGCCCTTCCT GCCCCAGCTG
    CAGACCACCT TCACCAAAGC CCTGCAGGAC TCTAACCGCG GCGTGCGCCT GAAGGCCGCC
    GATGCGCTAG GAAAGCTCAT TTCCATCCAC ATCAAGGTGG ATCCTCTTTT CATGGAGCTG
    CTGAATGGCA TCCGGGTCAT GGAGGACCCA GGCGTCAGGG ACACCATGCT GCAGGCCCTG
    AGGTTTGTGA TTCAGGGAGC GGGGGCCAAA GTGGATGTGG TCATCCGGAA AAACATCGTC
    TCGCTCCTGC TGAGCATGCT GGGACACGAC GAGGACAACA CGCGCATCTC CTCGGCCGGG
    TGCCTGGGGG AGCTATGTGC CTTTCTGACG GAAGAGGAGC TCAGCACTGT TCTTCAGCAG
    TACTTGCTGG CGGATGTGTC TGGCATTGAC TGGATGGTTC GGCATGGGCG GAGCCTGGCT
    CTCTCCGTGG CTGTGAGCGT GGCTCCTGGC AGACTCTGCG TAGGCAAGTA TGGCAGCGAC
    GTCCAGGACA TGATCCTCAG CAATGCCATG GCAGACAGGA TCCCCATCGC CGTGAGTGGG
    GTCCGGGGCA TGGGCTTCCT GATGAAGCAC CACATCGAGA CCGGAGGAGG ACAGTTGCCG
    GCCAAACTCT CCAGCCTGTT CATTAAGTGT CTGCAGAACC CATCCAGTGA CATCAGGTTG
    GTGGCTGAGA AGATGATCTG GTGGGCAAAC AAGGACCCGC TGCCTGCCCT GGACCCCCAG
    GCCATCAAGC CCATCCTGAA GGCCCTCCTG GACAACACCA AGGATAAAAA CACCGTCGTC
    AGGGCCTACA GCGACCAGGC GATTGTCAAC CTCCTCAAGA TGAGGCAGGG AGAGGAGGTG
    TTTCAGTCCC TCTGCAAGAT CCTGGATGTG GCCAGCTTGG AAGTGCTGAA TGAATGCAAC
    CGGAGGTCTC TGAAGAAGCT GGCCGGCCAG GCTGACTCCA CAGAGCAGGT GGACGACACC
    ATCCTGACCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833544.1
    CDS60..7931
    Translation

    Target ORF information:

    RefSeq Version XM_024977776.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GCN1, eIF2 alpha kinase activator homolog (GCN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977776.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGGCGGCGG ACACGCAGGT TTCTGAGACA CTAAAGCGTT TTGCAGGGAA GGTGACGACA 
    GCCAGTGTGA AGGAACGGAG AGAAATCCTC AGTGAGCTGG GGAAATGTGT TGCCGGAAAA
    GATCTGCCGG AGGGAGCTGT GAAAGGCCTC TGCAAACTCT TCTGCTTGAC CCTGCATCGA
    TACAGGGATG CAGCCTCACG CCGGGCCCTG CAGGCAGCCA TCCAGCAGCT GGCTGAGGCC
    CAGCCTGAAG CCACTGCCAA GAACCTTCTG CACTCTCTGC AGTCCTCGGG CATCGGCTCC
    AAAGCGGGTG TCCCCAGTAA GAGCAGCGGC TCGGCTGCCT TGCTGGCCCT GTCCTGGACT
    GGCCTACTGG TGCGCATCGT CTTTCCCTCG AGAGCCAAGC GGCAAGGAGA CATCTGGAAC
    AAACTGGTGG AGGTGCAGTG CCTGCTCCTG CTGGAGGTGC TGGGTGGCTC CCACAAGCAC
    GCCGTGGACA GTGCCGTGAA GAAGCTCACC AAGCTGTGGA GAGAGAACCC CGGGCTGGTG
    GAGCAGTACT TGTCAGCCAT TCTCAGCCTG GAGCCCAACC AGAGCTACGC AGGCATGCTG
    GGGCTGCTGG TGCAGTTCTG CACGAGCCAC AAGGAGATGG ACGTGGCCAA CCAGCACAAG
    AGTGCCCTGC TGGACTTTTA CGTGAAGAAC ATCCTGATGA GCAAAGTGAA GCCTCAGAAG
    TATCTGTTGG ATAACTGTGC CCCTTTGCTC CGGTACGTGT CCCACTCGGA GTTTAAGGAT
    CTGGTCCTGC CCACCATACA GAAGTCCTTA CTGAGGAGTC CAGAGAATGT CATTGAAACT
    ATCTCCAGTC TGCTGGCATC CGTGACTCTG GATCTCAGCC AGTATGCCCT GGACATTGGG
    AAAGGACTGG CTGGTCAGCT GAAGTCCAAC AGCCCTCGCT TGATGGATGA AGCCGTGCTG
    GCGCTGCGGA ACCTGGCCCG CCAGTGCAGT GACTCCTCGG CGATGGAAGC CCTGGCCAGG
    CACCTGTTCG CTATCCTCGG AGGCTCGGAG GGGAAGCTAA CTATTGTAGC CCAGAAGATG
    AGCGTTCTCT CAGGGATCGG GAGTGTCAGT CATCACGTGG TGTCGGGGCC TTCTAGTCAA
    GTCCTGAGCG GGACCGTGGC TGAGCTCTTC ATCCCGTTCC TCCAGCAAGA AGTTCATGAA
    GGCACCTTGG TGCACGCTGT CTCTGTCTTG GCTCTCTGGT GTCACCGCTT CACCACGGAA
    GTACCCAAGA AGCTCACCGA ATGGTTCAAG AAAGCCTTCA GCCTTAAAAC CTCCACCTCT
    GCCGTGAGGC ACGCCTACCT GCAGTGCATG TTGGTCTGCT TTCGAGGCGA CACACTGCTG
    CAGGCCCTGG ACTTGCTGCC CTTGCTCATC CAGACAGTGG AGAAGGCGGC CTCCCAAGGC
    ACTCAGGTTC CCGTTGTCAC CGAAGGGGTC GCCGCTGCCT TGCTGCTCAC GAAGCTCTCA
    ATGGCCGACT CGCAGGCTGA GGCCAAACTG AGTGGTTTCT GGCAGCTCAT TTTGGATGAG
    AAGAAGCAGG TTTTCACTTC CGAGAAATTC CTCCTCATGG CTTCAGAGGA TGCCCTGTGT
    ACCGTGCTGC ACCTGACAGA GAGACTGTTT CTTGACCATC TGCACCGACT CACAGGCAAC
    AAAGTTCAGC AGTACCACCG GGCCCTGGCG GCCGTGCTCC TGAGCCGCCC CTGGCACGTG
    CGCAGGCAGG CCCAGCAGAC GGCCCGGAAG CTGCTGTCCT CTCTCGGGGG CTTCAAGCTG
    GCTTACGGAC TGCTGGAGGA GCTGAAGACC GTCCTCAGTT CTCACAAGGT GCTGCCCTTC
    GAGGCTCTGG TGACTGACAC TGGGGAGGTG ACTGAGGCGG GCAAGGCCTA CGTGCCCCCG
    CGGGTCCTGC AGGAGGCTCT CTGTGTCATC TCCGGGGTGC CAGGGCTGGA GGGTGGCATC
    ACCGGCATGG AACAGCTGGC ACAGGAGATG CTGATCATCT CTCACCACCC ATCCTTAGTG
    GCTGTGCAGT CTGGCCTCTG GCCAGCACTT CTCGCCAGGA TGAAGATCGA TCCGGAAGCC
    TTCATTAGTA GGCACTTGGA CCAGATCATC CCCAGGATCA CCACCCAGAA CCCCTTGAAC
    CAGTCTTCCA TGAACGCCAT GGGCTCCCTC TCGATCCTGT CCCCAGACCG GGTCCTCCCG
    CAGCTCATCA GCACCATCAC CACCTCCGTG CAGAACCCAG CACTGTGCCA GGTGACGCGG
    GAGGAGTTTG CTATCATGCA GACCCCTGCA GGGGAGTTGT ACGACAAGTC CATCATTCAG
    AGTGCCCAGC AGGACAGCAT AAAAAAGGCC AACATGAAGC GAGAGAACAA AGCTTACTCC
    TTCAAGGAGC AGATCATCGA GTTGGAGCTG AAGGAGGAGA TAAAGAAGAA GAAAGGCATA
    AAAGAGGAGG TGCAGCTGAC CAGCAAACAG AAGGAGATGC TGCAGGCCCA GCTGGACAAG
    GAGGCGCAGA TCCGGAGGCG GCTGCAGGAG CTGGACAGTG AGCTGGAGGC GGCTCTGGGG
    CTGCTGGACA CCATCCTGGC CAAGAACCCG TCCGGCCTGA CCCAGTACAT CCCGGTCCTG
    GTCGACTCTT TCCTGCCCTT GCTGAAGTCT CCCCTGGCTG CTCCCAGGAT CAAGACCCCT
    TTCCTGTCCT TGGCTGCCTG TGTCATGCCC CCGAGGCTCA AGGCTTTGGG CACTTTGGTG
    AGCCATGTGA CTCTGCGCCT GCTGAAGCCG GAATGTGCCC TGGACGAGTC ATGGTGCCAG
    GAAGAGCTGT CGGTGGCCGT GAAGAGAGCA GTGACCCTGC TGCACAGTCA CACCATCACC
    AGCAGGGCAG GCAAGGGCGA GCCAGACGCT GCGCCCCTGT CCGCGCCAGC CTTCTCCTTG
    GTTTTCCCGT TTCTGAAGAT GGTGCTGACT GAGATGCCCC ACCACAGTGA GGAGGAGGAG
    GAAAGAATGG CCCAGATTCT TCAGATCCTC ACAGTCCACG CCCAGCTGCG GGCCTCCCCA
    AGCAACCCAC CTGGGCGTGT GGACGAGAAC GGCCCGGAGT TGCTGCCTCG CGTGGCCATG
    CTGCGCCTTC TGACCTGGGT GATTGGGACG GGCTCTCCCC GACTTCAGGT TCTGGCTTCA
    GAGGCCCTGA CCACCCTGTG TGCCAGCAGC AGTGGCGAGG ATGGCTGTGC CTTTGCAGAG
    CAGGAGGAGG TGGACGTGCT GCTCTGCGCC CTGCTGTCCC CCTGTGCCAA CGTGCGGGAC
    ACTGTACTCC GGGGCCTGTT GGAACTCCAC ATGGTGTTGC CGGCGCCTGA CACCGATGAG
    AAGAATGGCC TGAACCTTCT CCGCAGGCTC TGGGTGGTTA AGTTCGACAA GGAGGAAGAG
    ATCCGAAAGT TGGCCGAGAG GCTCTGGTCG ACCATGGGCC TAGATCTGCA GCCAGACCTC
    TGCTCCTTGC TCATCGACGA CGTCATCTAT CACGAGGCAG CTGTGAGGCA GGCCGGGGCG
    GAAGCCCTCT CCCAGGCGGT GGCGCGTTAC CAGCGGCAGG CGGCTGAGGT CATGGGCAGG
    CTCATGGAGA TCTACCAGGA GAAGCTCTAT CGGCCGCCCC CGGTGCTGGA TGCTCTGGGA
    CGAGTTATCT CCGAATCTCC TCCAGACCAG TGGGAAGCCA GGTGTGGCTT GGCTTTGGCC
    CTGAACAAGC TCTCCCAGTG TTTGGACAGC TCTCAGGTGA AGCCACTCTT TCAATTTTTT
    GTCCCCGACG CCCTCAACGA CCGAAACCCA GATGTCCGGA AGTGCATGTT GGATGCAGCT
    CTTGCGACAC TCAACACACA TGGAAAGGAG AACGTCAACT CGCTGCTGCC GGTGTTCGAG
    GAGTTCCTGA AGAACGCGCC GAACGATGCC AGCTACGATG CCGTGCGGCA GAGCGTGGTG
    GTCCTCATGG GCTCTCTAGC CAAGCACCTG GACAAGAGTG ACCCCAAAGT GAAGCCGATT
    GTCGCCAAGC TCATTGCTGC CCTCGCCACC CCCTCCCAGC AGGTCCAGGA ATCCGTGGCC
    AACTGCTTGC CACCCCTCGT CCCTGCCATA AAGGAAGACG CGGGGGGTAT GATCCAGAGG
    CTGATGCAGC AGCTGCTGGA GTCAGACAAG TACGCCGAGC GCAAAGGGGC TGCGTACGGG
    CTGGCGGGCC TGGTGAAGGG CCTGGGCATC CTGTCGCTGA AGCAGCAGGA GATGATGGCC
    GCGCTGACCG ATGCCATTCA GGACAAGAAG AACTTCCGCC GCCGGGAGGG AGCCCTCTTT
    GCCTTCGAGA TGCTCTGCAC CATGCTGGGG AAGCTCTTTG AGCCGTATGT GGTCCACGTG
    CTGCCCCATC TGCTGCTCTG CTTTGGGGAC GGGAACCAGT ATGTGCGGGA GGCTGCAGAT
    GACTGTGCCA AGGCTGTGAT GAGCAACCTG AGTGCTCATG GGGTGAAGCT GGTGCTCCCC
    TCCTTGTTGG CTGCCCTGGA GGAGGAATCC TGGAGAACCA AAGCCGGGTC GGTGGAGTTG
    CTCGGGGCCA TGGCCTACTG TGCTCCCAAG CAGCTGTCGT CCTGCCTACC CAACATTGTG
    CCCAAGCTCA CAGAGGTGCT CACCGACTCC CACGTCAAAG TGCAGAAGGC TGGGCAGCAG
    GCGCTCAGGC AGATCGGCTC TGTCATCAGG AACCCAGAGA TCCTGGCCAT CGCCCCGGTA
    CTGCTGGACG CCCTGACAGA CCCCTCCCGG AAGACCCAGA AGTGCTTGCA GACCTTGCTG
    GACACCAAGT TTGTCCACTT CATCGATGCA CCGTCCCTGG CCCTCATCAT GCCCATCGTC
    CAGAGAGCCT TCCAGGACCG TTCCACGGAC ACACGGAAGA TGGCCGCCCA GATCATTGGC
    AACATGTACT CCCTGACAGA CCAGAAGGAC TTGGCTCCTT ACCTGCCAAG CGTGACGCCT
    GGTCTGAAAG CCTCTCTCCT GGATCCTGTG CCTGAGGTAC GGACAGTGTC TGCAAAGGCT
    CTTGGGGCCA TGGTCAAGGG CATGGGGGAG TCATGCTTTG AGGACTTGCT GCCGTGGCTG
    ATGGAGACAC TGACCTACGA GCAGAGCTCT GTGGATCGCT CAGGCGCCGC GCAGGGGTTG
    GCTGAGGTCA TGGCCGGCTT GGGGGTGGAG AAGTTGGAGA AGCTGATGCC AGAAATTGTG
    GCTACAGCCA GCAAAGTGGA CATTGCCCCC CACGTCCGAG ACGGCTACAT CATGATGTTC
    AACTACCTGC CCATCACCTT CGGGGACAAA TTCACTCCTT ATGTGGGGCC CATCATCCCC
    TGTATCCTCA AAGCTCTTGC TGATGAGAAC GAGTTTGTGC GTGATACTGC CCTGCGTGCA
    GGCCAGCGGG TCATCTCCAT GTATGCTGAG ACGGCCATTG CCCTGCTGCT GCCCCAGCTG
    GAGCAAGGCC TCTTTGATGA CCTCTGGAGA ATAAGGTTCA GCTCTGTTCA GCTCCTTGGC
    GATCTCCTGT TCCACATCTC AGGAGTCACT GGGAAGATGA CCACAGAAAC TGCCTCAGAG
    GATGACAACT TTGGAACCGC GCAGTCCAAC AAGGCGATTA TCACCGCCCT GGGGGTAGAT
    CGGCGGAACC GGGTGTTGGC GGGGCTGTAC ATGGGCCGCT CGGACACCCA GCTGGTGGTG
    CGGCAGGCCT CCCTGCACGT CTGGAAGATT GTGGTCTCTA ACACCCCCCG CACCTTGCGT
    GAGATCCTTC CCACCCTTTT TGGGCTCCTG CTGGGTTTCC TGGCCAGCAC ATGCGCGGAT
    AAGAGAACAA TCGCAGCCAG GACGCTGGGA GATCTCGTCC GGAAGTTAGG GGAGAAAATC
    CTGCCGGAGA TCATCCCCAT CCTGGAGGAA GGCCTGAGAT CTCCCAAGAG TGACGAGAGG
    CAGGGCGTGT GCATCGGGCT GAGCGAGATC ATGAAGTCCA CCAGCCGGGA TGCGGTGCTG
    TATTTCTCCG AGTCCCTCGT GCCCACGGCG AGGAAGGCTT TGTGTGACCC GCTGGAGGAG
    GTCAGAGAGG CGGCGGCCAA GACGTTTGAG CAGCTGCACT CCACCATCGG CCACCAGGCC
    CTGGAGGACA TCCTCCCGTT TTTACTGAAG CAGCTGGATG ACGAGGAGGT GTCCGAGTTT
    GCCTTGGATG GTCTGAAGCA GGTGATGGCC ATCAAGAGTC GTGTGGTGCT GCCCTACCTC
    GTGCCCAAGG AGATGGCCAA CTGTCAGGCC GTGATCCTGT CCGTGGAGGA CGACACCGGG
    CACCGGATCA TCATCGAGGA CCTGTTGGAA GCCACCCGCA GCCCTGAGGT GGGCATGAGG
    CAGGCCGCGG CCATCATCCT CAACATCTAC TGCTCACGCT CCAAGGCGGA CTACACTAGC
    CACCTGCGGA GCCTGGTCTC AGGCCTGATC CGCCTCTTCA ACGACTCCAG CCCCGTGGTT
    CTGGAGGAGA GCTGGGACGC CCTGAATGCC ATCACCAAGA AACTGGATGC AGGCAACCAG
    CTGGCCCTCA TTGAGGAACT GCACAAGGAA ATCCGGTTCA TAGGAAATGA GAGCAGAGGC
    GAGCACGTGC CAGGCTTCTG CCTCCCGAAG AAGGGGGTAA CGTCCATCCT TCCGGTGCTG
    CGGGAGGGGG TCCTGACTGG CAGCCCGGAA CAGAAGGAGG AAGCTGCCAA AGCGTTAGGC
    TTGGTGATCC GCCTGACCTC GGCGGACGCC CTGCGGCCAT CCGTGGTCAA CATCACTGGC
    CCTCTGATTC GCATCCTGGG GGACCGGTTC AGCTGGAACG TGAAGGCGGC TCTGCTCGAG
    ACACTCAGCC TCTTGCTGGC CAAGGTCGGG ATCGCCCTGA AGCCCTTCCT GCCCCAGCTG
    CAGACCACCT TCACCAAAGC CCTGCAGGAC TCTAACCGCG GCGTGCGCCT GAAGGCCGCC
    GATGCGCTAG GAAAGCTCAT TTCCATCCAC ATCAAGGTGG ATCCTCTTTT CATGGAGCTG
    CTGAATGGCA TCCGGGTCAT GGAGGACCCA GGCGTCAGGG ACACCATGCT GCAGGCCCTG
    AGGTTTGTGA TTCAGGGAGC GGGGGCCAAA GTGGATGTGG TCATCCGGAA AAACATCGTC
    TCGCTCCTGC TGAGCATGCT GGGACACGAC GAGGACAACA CGCGCATCTC CTCGGCCGGG
    TGCCTGGGGG AGCTATGTGC CTTTCTGACG GAAGAGGAGC TCAGCACTGT TCTTCAGCAG
    TACTTGCTGG CGGATGTGTC TGGCATTGAC TGGATGGTTC GGCATGGGCG GAGCCTGGCT
    CTCTCCGTGG CTGTGAGCGT GGCTCCTGGC AGACTCTGCG TAGGCAAGTA TGGCAGCGAC
    GTCCAGGACA TGATCCTCAG CAATGCCATG GCAGACAGGA TCCCCATCGC CGTGAGTGGG
    GTCCGGGGCA TGGGCTTCCT GATGAAGCAC CACATCGAGA CCGGAGGAGG ACAGTTGCCG
    GCCAAACTCT CCAGCCTGTT CATTAAGTGT CTGCAGAACC CATCCAGTGA CATCAGGTTG
    GTGGCTGAGA AGATGATCTG GTGGGCAAAC AAGGACCCGC TGCCTGCCCT GGACCCCCAG
    GCCATCAAGC CCATCCTGAA GGCCCTCCTG GACAACACCA AGGATAAAAA CACCGTCGTC
    AGGGCCTACA GCGACCAGGC GATTGTCAAC CTCCTCAAGA TGAGGCAGGG AGAGGAGGTG
    TTTCAGTCCC TCTGCAAGAT CCTGGATGTG GCCAGCTTGG AAGTGCTGAA TGAATGCAAC
    CGGAGGTCTC TGAAGAAGCT GGCCGGCCAG GCTGACTCCA CAGAGCAGGT GGACGACACC
    ATCCTGACCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09078
    Clone ID Related Accession (Same CDS sequence) XM_002694522.4 , XM_616264.8
    Accession Version XM_002694522.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8016bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product translational activator GCN1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002694522.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCGGCGG ACACGCAGGT TTCTGAGACA CTAAAGCGTT TTGCAGGGAA GGTGACGACA 
    GCCAGTGTGA AGGAACGGAG AGAAATCCTC AGTGAGCTGG GGAAATGTGT TGCCGGAAAA
    GATCTGCCGG AGGGAGCTGT GAAAGGCCTC TGCAAACTCT TCTGCTTGAC CCTGCATCGA
    TACAGGGATG CAGCCTCACG CCGGGCCCTG CAGGCAGCCA TCCAGCAGCT GGCTGAGGCC
    CAGCCTGAAG CCACTGCCAA GAACCTTCTG CACTCTCTGC AGTCCTCGGG CATCGGCTCC
    AAAGCGGGTG TCCCCAGTAA GAGCAGCGGC TCGGCTGCCT TGCTGGCCCT GTCCTGGACT
    GGCCTACTGG TGCGCATCGT CTTTCCCTCG AGAGCCAAGC GGCAAGGAGA CATCTGGAAC
    AAACTGGTGG AGGTGCAGTG CCTGCTCCTG CTGGAGGTGC TGGGTGGCTC CCACAAGCAC
    GCCGTGGACA GTGCCGTGAA GAAGCTCACC AAGCTGTGGA GAGAGAACCC CGGGCTGGTG
    GAGCAGTACT TGTCAGCCAT TCTCAGCCTG GAGCCCAACC AGAGCTACGC AGGCATGCTG
    GGGCTGCTGG TGCAGTTCTG CACGAGCCAC AAGGAGATGG ACGTGGCCAA CCAGCACAAG
    AGTGCCCTGC TGGACTTTTA CGTGAAGAAC ATCCTGATGA GCAAAGTGAA GCCTCAGAAG
    TATCTGTTGG ATAACTGTGC CCCTTTGCTC CGGTACGTGT CCCACTCGGA GTTTAAGGAT
    CTGGTCCTGC CCACCATACA GAAGTCCTTA CTGAGGAGTC CAGAGAATGT CATTGAAACT
    ATCTCCAGTC TGCTGGCATC CGTGACTCTG GATCTCAGCC AGTATGCCCT GGACATTGGG
    AAAGGACTGG CTGGTCAGCT GAAGTCCAAC AGCCCTCGCT TGATGGATGA AGCCGTGCTG
    GCGCTGCGGA ACCTGGCCCG CCAGTGCAGT GACTCCTCGG CGATGGAAGC CCTGGCCAGG
    CACCTGTTCG CTATCCTCGG AGGCTCGGAG GGGAAGCTAA CTATTGTAGC CCAGAAGATG
    AGCGTTCTCT CAGGGATCGG GAGTGTCAGT CATCACGTGG TGTCGGGGCC TTCTAGTCAA
    GTCCTGAGCG GGACCGTGGC TGAGCTCTTC ATCCCGTTCC TCCAGCAAGA AGTTCATGAA
    GGCACCTTGG TGCACGCTGT CTCTGTCTTG GCTCTCTGGT GTCACCGCTT CACCACGGAA
    GTACCCAAGA AGCTCACCGA ATGGTTCAAG AAAGCCTTCA GCCTTAAAAC CTCCACCTCT
    GCCGTGAGGC ACGCCTACCT GCAGTGCATG TTGGTCTGCT TTCGAGGCGA CACACTGCTG
    CAGGCCCTGG ACTTGCTGCC CTTGCTCATC CAGACAGTGG AGAAGGCGGC CTCCCAAGGC
    ACTCAGGTTC CCGTTGTCAC CGAAGGGGTC GCCGCTGCCT TGCTGCTCAC GAAGCTCTCA
    ATGGCCGACT CGCAGGCTGA GGCCAAACTG AGTGGTTTCT GGCAGCTCAT TTTGGATGAG
    AAGAAGCAGG TTTTCACTTC CGAGAAATTC CTCCTCATGG CTTCAGAGGA TGCCCTGTGT
    ACCGTGCTGC ACCTGACAGA GAGACTGTTT CTTGACCATC CGCACCGACT CACAGGCAAC
    AAAGTTCAGC AGTACCACCG GGCCCTGGCG GCCGTGCTCC TGAGCCGCCC CTGGCACGTG
    CGCAGGCAGG CCCAGCAGAC GGCCCGGAAG CTGCTGTCCT CTCTCGGGGG CTTCAAGCTG
    GCTTACGGAC TGCTGGAGGA GCTGAAGACC GTCCTCAGTT CTCACAAGGT GCTGCCCTTC
    GAGGCTCTGG TGACCGACAC TGGGGAGGTG ACTGAGGCGG GCAAGGCCTA CGTGCCCCCG
    CGGGTCCTGC AGGAGGCTCT CTGTGTCATC TCCGGGGTGC CAGGGCTGGA GGGTGGCATC
    ACCGGCATGG AACAGCTGGC ACAGGAGATG CTGATCATCT CTCACCACCC ATCCTTAGTG
    GCTGTGCAGT CTGGCCTCTG GCCAGCACTT CTCGCCAGGA TGAAGATCGA TCCGGAAGCC
    TTCATTAGTA GGCACTTGGA CCAGATCATC CCCAGGATCA CCACCCAGAA CCCCTTGAAC
    CAGTCTTCCA TGAACGCCAT GGGCTCCCTC TCGATCCTGT CCCCAGACCG GGTCCTCCCG
    CAGCTCATCA GCACCATCAC CACCTCCGTG CAGAACCCAG CACTGTGCCA GGTGACGCGG
    GAGGAGTTTG CTATCATGCA GACCCCTGCA GGGGAGTTGT ACGACAAGTC CATCATTCAG
    AGTGCCCAGC AGGACAGCAT AAAAAAGGCC AACATGAAGC GAGAGAACAA AGCTTACTCC
    TTCAAGGAGC AGATCATCGA GTTGGAGCTG AAGGAGGAGA TAAAGAAGAA GAAAGGCATA
    AAAGAGGAGG TGCAGCTGAC CAGCAAACAG AAGGAGATGC TGCAGGCCCA GCTGGACAAG
    GAGGCGCAGA TCCGGAGGCG GCTGCAGGAG CTGGACAGTG AGCTGGAGGC GGCTCTGGGG
    CTGCTGGACA CCATCCTGGC CAAGAACCCG TCCGGCCTGA CCCAGTACAT CCCGGTCCTG
    GTCGACTCTT TCCTGCCCTT GCTGAAGTCT CCCCTGGCTG CTCCCAGGAT CAAGACCCCT
    TTCCTGTCCT TGGCTGCCTG TGTCATGCCC CCGAGGCTCA AGGCTTTGGG CACTTTGGTG
    AGCCATGTGA CTCTGCGCCT GCTGAAGCCG GAATGTGCCC TGGACGAGTC ATGGTGCCAG
    GAAGAGCTGT CGGTGGCCGT GAAGAGAGCA GTGACCCTGC TGCACAGTCA CACCATCACC
    AGCAGGGCAG GCAAGGGCGA GCCAGACGCT GCGCCCCTGT CCGCGCCAGC CTTCTCCTTG
    GTTTTCCCGT TTCTGAAGAT GGTGCTGACT GAGATGCCCC ACCACAGTGA GGAGGAGGAG
    GAAAGAATGG CCCAGATTCT TCAGATCCTC ACAGTCCACG CCCAGCTGCG GGCCTCCCCA
    AGCAACCCAC CTGGGCGTGT GGACGAGAAC GGCCCGGAGT TGCTGCCTCG CGTGGCCATG
    CTGCGCCTTC TGACCTGGGT GATTGGGACG GGCTCTCCCC GACTTCAGGT TCTGGCTTCA
    GAGGCCCTGA CCACCCTGTG TGCCAGCAGC AGTGGCGAGG ATGGCTGTGC CTTTGCAGAG
    CAGGAGGAGG TGGACGTGCT GCTCTGCGCC CTGCTGTCCC CCTGTGCCAA CGTGCGGGAC
    ACTGTACTCC GGGGCCTGTT GGAACTCCAC ATGGTGTTGC CGGCGCCTGA CACCGATGAG
    AAGAATGGCC TGAACCTTCT CCGCAGGCTC TGGGTGGTTA AGTTCGACAA GGAGGAAGAG
    ATCCGAAAGT TGGCCGAGAG GCTCTGGTCG ACCATGGGCC TAGATCTGCA GCCAGACCTC
    TGCTCCTTGC TCATCGACGA CGTCATCTAT CACGAGGCAG CTGTGAGGCA GGCCGGGGCG
    GAAGCCCTCT CCCAGGCGGT GGCGCGTTAC CAGCGGCAGG CGGCTGAGGT CATGGGCAGG
    CTCATGGAGA TCTACCAGGA GAAGCTCTAT CGGCCGCCCC CGGTGCTGGA TGCTCTGGGA
    CGAGTTATCT CCGAATCTCC TCCAGACCAG TGGGAAGCCA GGTGTGGCTT GGCTTTGGCC
    CTGAACAAGC TCTCCCAGTG TTTGGACAGC TCTCAGGTGA AGCCACTCTT TCAATTTTTT
    GTCCCCGACG CCCTCAACGA CCGAAACCCA GATGTCCGGA AGTGCATGTT GGATGCAGCT
    CTTGCGACAC TCAACACACA TGGAAAGGAG AACGTCAACT CGCTGCTGCC GGTGTTCGAG
    GAGTTCCTGA AGAACGCGCC GAACGATGCC AGCTACGATG CCGTGCGGCA GAGCGTGGTG
    GTCCTCATGG GCTCTCTAGC CAAGCACCTG GACAAGAGTG ACCCCAAAGT GAAGCCGATT
    GTCGCCAAGC TCATTGCTGC CCTCGCCACC CCCTCCCAGC AGGTCCAGGA ATCCGTGGCC
    AACTGCTTGC CACCCCTCGT CCCTGCCATA AAGGAAGACG CGGGGGGTAT GATCCAGAGG
    CTGATGCAGC AGCTGCTGGA GTCAGACAAG TACGCCGAGC GCAAAGGGGC TGCGTACGGG
    CTGGCGGGCC TGGTGAAGGG CCTGGGCATC CTGTCGCTGA AGCAGCAGGA GATGATGGCC
    GCGCTGACCG ATGCCATTCA GGACAAGAAG AACTTCCGCC GCCGGGAGGG AGCCCTCTTT
    GCCTTCGAGA TGCTCTGCAC CATGCTGGGG AAGCTCTTTG AGCCGTATGT GGTCCACGTG
    CTGCCCCATC TGCTGCTCTG CTTTGGGGAC GGGAACCAGT ATGTGCGGGA GGCTGCAGAT
    GACTGTGCCA AGGCTGTGAT GAGCAACCTG AGTGCTCATG GGGTGAAGCT GGTGCTCCCC
    TCCTTGTTGG CTGCCCTGGA GGAGGAATCC TGGAGAACCA AAGCCGGGTC GGTGGAGTTG
    CTCGGGGCCA TGGCCTACTG TGCTCCCAAG CAGCTGTCGT CCTGCCTACC CAACATTGTG
    CCCAAGCTCA CAGAGGTGCT CACCGACTCC CACGTCAAAG TGCAGAAGGC TGGGCAGCAG
    GCGCTCAGGC AGATCGGCTC TGTCATCAGG AACCCAGAGA TCCTGGCCAT CGCCCCGGTA
    CTGCTGGACG CCCTGACAGA CCCCTCCCGG AAGACCCAGA AGTGCTTGCA GACCTTGCTG
    GACACCAAGT TTGTCCACTT CATCGATGCA CCGTCCCTGG CCCTCATCAT GCCCATCGTC
    CAGAGAGCCT TCCAGGACCG TTCCACGGAC ACACGGAAGA TGGCCGCCCA GATCATTGGC
    AACATGTACT CCCTGACAGA CCAGAAGGAC TTGGCTCCTT ACCTGCCAAG CGTGACGCCT
    GGTCTGAAAG CCTCTCTCCT GGATCCTGTG CCTGAGGTAC GGACAGTGTC TGCAAAGGCT
    CTTGGGGCCA TGGTCAAGGG CATGGGGGAG TCATGCTTTG AGGACTTGCT GCCGTGGCTG
    ATGGAGACAC TGACCTACGA GCAGAGCTCT GTGGATCGCT CAGGCGCCGC GCAGGGGTTG
    GCTGAGGTCA TGGCCGGCTT GGGGGTGGAG AAGTTGGAGA AGCTGATGCC AGAAATTGTG
    GCTACAGCCA GCAAAGTGGA CATTGCCCCC CACGTCCGAG ACGGCTACAT CATGATGTTC
    AACTACCTGC CCATCACCTT CGGGGACAAA TTCACTCCTT ATGTGGGGCC CATCATCCCC
    TGTATCCTCA AAGCTCTTGC TGATGAGAAC GAGTTTGTGC GTGATACTGC CCTGCGTGCA
    GGCCAGCGGG TCATCTCCAT GTATGCTGAG ACGGCCATTG CCCTGCTGCT GCCCCAGCTG
    GAGCAAGGCC TCTTTGATGA CCTCTGGAGA ATAAGGTTCA GCTCTGTTCA GCTCCTTGGC
    GATCTCCTGT TCCACATCTC AGGAGTCACT GGGAAGATGA CCACAGAAAC TGCCTCAGAG
    GATGACAACT TTGGAACCGC GCAGTCCAAC AAGGCGATTA TCACCGCCCT GGGGGTAGAT
    CGGCGGAACC GGGTGTTGGC GGGGCTGTAC ATGGGCCGCT CGGACACCCA GCTGGTGGTG
    CGGCAGGCCT CCCTGCACGT CTGGAAGATT GTGGTCTCTA ACACCCCCCG CACCTTGCGT
    GAGATCCTTC CCACCCTTTT TGGGCTCCTG CTGGGTTTCC TGGCCAGCAC ATGCGCGGAT
    AAGAGAACAA TCGCAGCCAG GACGCTGGGA GATCTCGTCC GGAAGTTAGG GGAGAAAATC
    CTGCCGGAGA TCATCCCCAT CCTGGAGGAA GGCCTGAGAT CTCCCAAGAG TGACGAGAGG
    CAGGGCGTGT GCATCGGGCT GAGCGAGATC ATGAAGTCCA CCAGCCGGGA TGCGGTGCTG
    TATTTCTCCG AGTCCCTCGT GCCCACGGCG AGGAAGGCTT TGTGTGACCC GCTGGAGGAG
    GTCAGAGAGG CGGCGGCCAA GACGTTTGAG CAGCTGCACT CCACCATCGG CCACCAGGCC
    CTGGAGGACA TCCTCCCGTT TTTACTGAAG CAGCTGGATG ACGAGGAGGT GTCCGAGTTT
    GCCTTGGATG GTCTGAAGCA GGTGATGGCC ATCAAGAGTC GTGTGGTGCT GCCCTACCTC
    GTGCCCAAGC TGACGACCCC GCCCGTCAAC ACCCGGGTGC TGGCTTTCCT TTCATCTGTG
    GCGGGTGATG CCCTGACACG TCATCTTGGC GTGATCCTCC CGGCGGTCAT GTTGGCCCTG
    AAGGAGAAGC TGGGGACTCC AGACGAGCAG CTGGAGATGG CCAACTGTCA GGCCGTGATC
    CTGTCCGTGG AGGACGACAC CGGGCACCGG ATCATCATCG AGGACCTGTT GGAAGCCACC
    CGCAGCCCTG AGGTGGGCAT GAGGCAGGCC GCGGCCATCA TCCTCAACAT CTACTGCTCA
    CGCTCCAAGG CGGACTACAC TAGCCACCTG CGGAGCCTGG TCTCAGGCCT GATCCGCCTC
    TTCAACGACT CCAGCCCCGT GGTTCTGGAG GAGAGCTGGG ACGCCCTGAA TGCCATCACC
    AAGAAACTGG ATGCAGGCAA CCAGCTGGCC CTCATTGAGG AACTGCACAA GGAAATCCGG
    TTCATAGGAA ATGAGAGCAG AGGCGAGCAC GTGCCAGGCT TCTGCCTCCC GAAGAAGGGG
    GTAACGTCCA TCCTTCCGGT GCTGCGGGAG GGGGTCCTGA CTGGCAGCCC GGAACAGAAG
    GAGGAAGCTG CCAAAGCGTT AGGCTTGGTG ATCCGCCTGA CCTCGGCGGA CGCCCTGCGG
    CCATCCGTGG TCAACATCAC TGGCCCTCTG ATTCGCATCC TGGGGGACCG GTTCAGCTGG
    AACGTGAAGG CGGCTCTGCT CGAGACACTC AGCCTCTTGC TGGCCAAGGT CGGGATCGCC
    CTGAAGCCCT TCCTGCCCCA GCTGCAGACC ACCTTCACCA AAGCCCTGCA GGACTCTAAC
    CGCGGCGTGC GCCTGAAGGC CGCCGATGCG CTAGGAAAGC TCATTTCCAT CCACATCAAG
    GTGGATCCTC TTTTCATGGA GCTGCTGAAT GGCATCCGGG TCATGGAGGA CCCAGGCGTC
    AGGGACACCA TGCTGCAGGC CCTGAGGTTT GTGATTCAGG GAGCGGGGGC CAAAGTGGAT
    GTGGTCATCC GGAAAAACAT CGTCTCGCTC CTGCTGAGCA TGCTGGGACA CGACGAGGAC
    AACACGCGCA TCTCCTCGGC CGGGTGCCTG GGGGAGCTAT GTGCCTTTCT GACGGAAGAG
    GAGCTCAGCA CTGTTCTTCA GCAGTACTTG CTGGCGGATG TGTCTGGCAT TGACTGGATG
    GTTCGGCATG GGCGGAGCCT GGCTCTCTCC GTGGCTGTGA GCGTGGCTCC TGGCAGACTC
    TGCGTAGGCA AGTATGGCAG CGACGTCCAG GACATGATCC TCAGCAATGC CATGGCAGAC
    AGGATCCCCA TCGCCGTGAG TGGGGTCCGG GGCATGGGCT TCCTGATGAA GCACCACATC
    GAGACCGGAG GAGGACAGTT GCCGGCCAAA CTCTCCAGCC TGTTCATTAA GTGTCTGCAG
    AACCCATCCA GTGACATCAG GTTGGTGGCT GAGAAGATGA TCTGGTGGGC AAACAAGGAC
    CCGCTGCCTG CCCTGGACCC CCAGGCCATC AAGCCCATCC TGAAGGCCCT CCTGGACAAC
    ACCAAGGATA AAAACACCGT CGTCAGGGCC TACAGCGACC AGGCGATTGT CAACCTCCTC
    AAGATGAGGC AGGGAGAGGA GGTGTTTCAG TCCCTCTGCA AGATCCTGGA TGTGGCCAGC
    TTGGAAGTGC TGAATGAATG CAACCGGAGG TCTCTGAAGA AGCTGGCCGG CCAGGCTGAC
    TCCACAGAGC AGGTGGACGA CACCATCCTG ACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002694568.1
    CDS27..8042
    Translation

    Target ORF information:

    RefSeq Version XM_002694522.4
    Organism Bos taurus(cattle)
    Definition Bos taurus GCN1, eIF2 alpha kinase activator homolog (GCN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002694522.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCGGCGG ACACGCAGGT TTCTGAGACA CTAAAGCGTT TTGCAGGGAA GGTGACGACA 
    GCCAGTGTGA AGGAACGGAG AGAAATCCTC AGTGAGCTGG GGAAATGTGT TGCCGGAAAA
    GATCTGCCGG AGGGAGCTGT GAAAGGCCTC TGCAAACTCT TCTGCTTGAC CCTGCATCGA
    TACAGGGATG CAGCCTCACG CCGGGCCCTG CAGGCAGCCA TCCAGCAGCT GGCTGAGGCC
    CAGCCTGAAG CCACTGCCAA GAACCTTCTG CACTCTCTGC AGTCCTCGGG CATCGGCTCC
    AAAGCGGGTG TCCCCAGTAA GAGCAGCGGC TCGGCTGCCT TGCTGGCCCT GTCCTGGACT
    GGCCTACTGG TGCGCATCGT CTTTCCCTCG AGAGCCAAGC GGCAAGGAGA CATCTGGAAC
    AAACTGGTGG AGGTGCAGTG CCTGCTCCTG CTGGAGGTGC TGGGTGGCTC CCACAAGCAC
    GCCGTGGACA GTGCCGTGAA GAAGCTCACC AAGCTGTGGA GAGAGAACCC CGGGCTGGTG
    GAGCAGTACT TGTCAGCCAT TCTCAGCCTG GAGCCCAACC AGAGCTACGC AGGCATGCTG
    GGGCTGCTGG TGCAGTTCTG CACGAGCCAC AAGGAGATGG ACGTGGCCAA CCAGCACAAG
    AGTGCCCTGC TGGACTTTTA CGTGAAGAAC ATCCTGATGA GCAAAGTGAA GCCTCAGAAG
    TATCTGTTGG ATAACTGTGC CCCTTTGCTC CGGTACGTGT CCCACTCGGA GTTTAAGGAT
    CTGGTCCTGC CCACCATACA GAAGTCCTTA CTGAGGAGTC CAGAGAATGT CATTGAAACT
    ATCTCCAGTC TGCTGGCATC CGTGACTCTG GATCTCAGCC AGTATGCCCT GGACATTGGG
    AAAGGACTGG CTGGTCAGCT GAAGTCCAAC AGCCCTCGCT TGATGGATGA AGCCGTGCTG
    GCGCTGCGGA ACCTGGCCCG CCAGTGCAGT GACTCCTCGG CGATGGAAGC CCTGGCCAGG
    CACCTGTTCG CTATCCTCGG AGGCTCGGAG GGGAAGCTAA CTATTGTAGC CCAGAAGATG
    AGCGTTCTCT CAGGGATCGG GAGTGTCAGT CATCACGTGG TGTCGGGGCC TTCTAGTCAA
    GTCCTGAGCG GGACCGTGGC TGAGCTCTTC ATCCCGTTCC TCCAGCAAGA AGTTCATGAA
    GGCACCTTGG TGCACGCTGT CTCTGTCTTG GCTCTCTGGT GTCACCGCTT CACCACGGAA
    GTACCCAAGA AGCTCACCGA ATGGTTCAAG AAAGCCTTCA GCCTTAAAAC CTCCACCTCT
    GCCGTGAGGC ACGCCTACCT GCAGTGCATG TTGGTCTGCT TTCGAGGCGA CACACTGCTG
    CAGGCCCTGG ACTTGCTGCC CTTGCTCATC CAGACAGTGG AGAAGGCGGC CTCCCAAGGC
    ACTCAGGTTC CCGTTGTCAC CGAAGGGGTC GCCGCTGCCT TGCTGCTCAC GAAGCTCTCA
    ATGGCCGACT CGCAGGCTGA GGCCAAACTG AGTGGTTTCT GGCAGCTCAT TTTGGATGAG
    AAGAAGCAGG TTTTCACTTC CGAGAAATTC CTCCTCATGG CTTCAGAGGA TGCCCTGTGT
    ACCGTGCTGC ACCTGACAGA GAGACTGTTT CTTGACCATC CGCACCGACT CACAGGCAAC
    AAAGTTCAGC AGTACCACCG GGCCCTGGCG GCCGTGCTCC TGAGCCGCCC CTGGCACGTG
    CGCAGGCAGG CCCAGCAGAC GGCCCGGAAG CTGCTGTCCT CTCTCGGGGG CTTCAAGCTG
    GCTTACGGAC TGCTGGAGGA GCTGAAGACC GTCCTCAGTT CTCACAAGGT GCTGCCCTTC
    GAGGCTCTGG TGACCGACAC TGGGGAGGTG ACTGAGGCGG GCAAGGCCTA CGTGCCCCCG
    CGGGTCCTGC AGGAGGCTCT CTGTGTCATC TCCGGGGTGC CAGGGCTGGA GGGTGGCATC
    ACCGGCATGG AACAGCTGGC ACAGGAGATG CTGATCATCT CTCACCACCC ATCCTTAGTG
    GCTGTGCAGT CTGGCCTCTG GCCAGCACTT CTCGCCAGGA TGAAGATCGA TCCGGAAGCC
    TTCATTAGTA GGCACTTGGA CCAGATCATC CCCAGGATCA CCACCCAGAA CCCCTTGAAC
    CAGTCTTCCA TGAACGCCAT GGGCTCCCTC TCGATCCTGT CCCCAGACCG GGTCCTCCCG
    CAGCTCATCA GCACCATCAC CACCTCCGTG CAGAACCCAG CACTGTGCCA GGTGACGCGG
    GAGGAGTTTG CTATCATGCA GACCCCTGCA GGGGAGTTGT ACGACAAGTC CATCATTCAG
    AGTGCCCAGC AGGACAGCAT AAAAAAGGCC AACATGAAGC GAGAGAACAA AGCTTACTCC
    TTCAAGGAGC AGATCATCGA GTTGGAGCTG AAGGAGGAGA TAAAGAAGAA GAAAGGCATA
    AAAGAGGAGG TGCAGCTGAC CAGCAAACAG AAGGAGATGC TGCAGGCCCA GCTGGACAAG
    GAGGCGCAGA TCCGGAGGCG GCTGCAGGAG CTGGACAGTG AGCTGGAGGC GGCTCTGGGG
    CTGCTGGACA CCATCCTGGC CAAGAACCCG TCCGGCCTGA CCCAGTACAT CCCGGTCCTG
    GTCGACTCTT TCCTGCCCTT GCTGAAGTCT CCCCTGGCTG CTCCCAGGAT CAAGACCCCT
    TTCCTGTCCT TGGCTGCCTG TGTCATGCCC CCGAGGCTCA AGGCTTTGGG CACTTTGGTG
    AGCCATGTGA CTCTGCGCCT GCTGAAGCCG GAATGTGCCC TGGACGAGTC ATGGTGCCAG
    GAAGAGCTGT CGGTGGCCGT GAAGAGAGCA GTGACCCTGC TGCACAGTCA CACCATCACC
    AGCAGGGCAG GCAAGGGCGA GCCAGACGCT GCGCCCCTGT CCGCGCCAGC CTTCTCCTTG
    GTTTTCCCGT TTCTGAAGAT GGTGCTGACT GAGATGCCCC ACCACAGTGA GGAGGAGGAG
    GAAAGAATGG CCCAGATTCT TCAGATCCTC ACAGTCCACG CCCAGCTGCG GGCCTCCCCA
    AGCAACCCAC CTGGGCGTGT GGACGAGAAC GGCCCGGAGT TGCTGCCTCG CGTGGCCATG
    CTGCGCCTTC TGACCTGGGT GATTGGGACG GGCTCTCCCC GACTTCAGGT TCTGGCTTCA
    GAGGCCCTGA CCACCCTGTG TGCCAGCAGC AGTGGCGAGG ATGGCTGTGC CTTTGCAGAG
    CAGGAGGAGG TGGACGTGCT GCTCTGCGCC CTGCTGTCCC CCTGTGCCAA CGTGCGGGAC
    ACTGTACTCC GGGGCCTGTT GGAACTCCAC ATGGTGTTGC CGGCGCCTGA CACCGATGAG
    AAGAATGGCC TGAACCTTCT CCGCAGGCTC TGGGTGGTTA AGTTCGACAA GGAGGAAGAG
    ATCCGAAAGT TGGCCGAGAG GCTCTGGTCG ACCATGGGCC TAGATCTGCA GCCAGACCTC
    TGCTCCTTGC TCATCGACGA CGTCATCTAT CACGAGGCAG CTGTGAGGCA GGCCGGGGCG
    GAAGCCCTCT CCCAGGCGGT GGCGCGTTAC CAGCGGCAGG CGGCTGAGGT CATGGGCAGG
    CTCATGGAGA TCTACCAGGA GAAGCTCTAT CGGCCGCCCC CGGTGCTGGA TGCTCTGGGA
    CGAGTTATCT CCGAATCTCC TCCAGACCAG TGGGAAGCCA GGTGTGGCTT GGCTTTGGCC
    CTGAACAAGC TCTCCCAGTG TTTGGACAGC TCTCAGGTGA AGCCACTCTT TCAATTTTTT
    GTCCCCGACG CCCTCAACGA CCGAAACCCA GATGTCCGGA AGTGCATGTT GGATGCAGCT
    CTTGCGACAC TCAACACACA TGGAAAGGAG AACGTCAACT CGCTGCTGCC GGTGTTCGAG
    GAGTTCCTGA AGAACGCGCC GAACGATGCC AGCTACGATG CCGTGCGGCA GAGCGTGGTG
    GTCCTCATGG GCTCTCTAGC CAAGCACCTG GACAAGAGTG ACCCCAAAGT GAAGCCGATT
    GTCGCCAAGC TCATTGCTGC CCTCGCCACC CCCTCCCAGC AGGTCCAGGA ATCCGTGGCC
    AACTGCTTGC CACCCCTCGT CCCTGCCATA AAGGAAGACG CGGGGGGTAT GATCCAGAGG
    CTGATGCAGC AGCTGCTGGA GTCAGACAAG TACGCCGAGC GCAAAGGGGC TGCGTACGGG
    CTGGCGGGCC TGGTGAAGGG CCTGGGCATC CTGTCGCTGA AGCAGCAGGA GATGATGGCC
    GCGCTGACCG ATGCCATTCA GGACAAGAAG AACTTCCGCC GCCGGGAGGG AGCCCTCTTT
    GCCTTCGAGA TGCTCTGCAC CATGCTGGGG AAGCTCTTTG AGCCGTATGT GGTCCACGTG
    CTGCCCCATC TGCTGCTCTG CTTTGGGGAC GGGAACCAGT ATGTGCGGGA GGCTGCAGAT
    GACTGTGCCA AGGCTGTGAT GAGCAACCTG AGTGCTCATG GGGTGAAGCT GGTGCTCCCC
    TCCTTGTTGG CTGCCCTGGA GGAGGAATCC TGGAGAACCA AAGCCGGGTC GGTGGAGTTG
    CTCGGGGCCA TGGCCTACTG TGCTCCCAAG CAGCTGTCGT CCTGCCTACC CAACATTGTG
    CCCAAGCTCA CAGAGGTGCT CACCGACTCC CACGTCAAAG TGCAGAAGGC TGGGCAGCAG
    GCGCTCAGGC AGATCGGCTC TGTCATCAGG AACCCAGAGA TCCTGGCCAT CGCCCCGGTA
    CTGCTGGACG CCCTGACAGA CCCCTCCCGG AAGACCCAGA AGTGCTTGCA GACCTTGCTG
    GACACCAAGT TTGTCCACTT CATCGATGCA CCGTCCCTGG CCCTCATCAT GCCCATCGTC
    CAGAGAGCCT TCCAGGACCG TTCCACGGAC ACACGGAAGA TGGCCGCCCA GATCATTGGC
    AACATGTACT CCCTGACAGA CCAGAAGGAC TTGGCTCCTT ACCTGCCAAG CGTGACGCCT
    GGTCTGAAAG CCTCTCTCCT GGATCCTGTG CCTGAGGTAC GGACAGTGTC TGCAAAGGCT
    CTTGGGGCCA TGGTCAAGGG CATGGGGGAG TCATGCTTTG AGGACTTGCT GCCGTGGCTG
    ATGGAGACAC TGACCTACGA GCAGAGCTCT GTGGATCGCT CAGGCGCCGC GCAGGGGTTG
    GCTGAGGTCA TGGCCGGCTT GGGGGTGGAG AAGTTGGAGA AGCTGATGCC AGAAATTGTG
    GCTACAGCCA GCAAAGTGGA CATTGCCCCC CACGTCCGAG ACGGCTACAT CATGATGTTC
    AACTACCTGC CCATCACCTT CGGGGACAAA TTCACTCCTT ATGTGGGGCC CATCATCCCC
    TGTATCCTCA AAGCTCTTGC TGATGAGAAC GAGTTTGTGC GTGATACTGC CCTGCGTGCA
    GGCCAGCGGG TCATCTCCAT GTATGCTGAG ACGGCCATTG CCCTGCTGCT GCCCCAGCTG
    GAGCAAGGCC TCTTTGATGA CCTCTGGAGA ATAAGGTTCA GCTCTGTTCA GCTCCTTGGC
    GATCTCCTGT TCCACATCTC AGGAGTCACT GGGAAGATGA CCACAGAAAC TGCCTCAGAG
    GATGACAACT TTGGAACCGC GCAGTCCAAC AAGGCGATTA TCACCGCCCT GGGGGTAGAT
    CGGCGGAACC GGGTGTTGGC GGGGCTGTAC ATGGGCCGCT CGGACACCCA GCTGGTGGTG
    CGGCAGGCCT CCCTGCACGT CTGGAAGATT GTGGTCTCTA ACACCCCCCG CACCTTGCGT
    GAGATCCTTC CCACCCTTTT TGGGCTCCTG CTGGGTTTCC TGGCCAGCAC ATGCGCGGAT
    AAGAGAACAA TCGCAGCCAG GACGCTGGGA GATCTCGTCC GGAAGTTAGG GGAGAAAATC
    CTGCCGGAGA TCATCCCCAT CCTGGAGGAA GGCCTGAGAT CTCCCAAGAG TGACGAGAGG
    CAGGGCGTGT GCATCGGGCT GAGCGAGATC ATGAAGTCCA CCAGCCGGGA TGCGGTGCTG
    TATTTCTCCG AGTCCCTCGT GCCCACGGCG AGGAAGGCTT TGTGTGACCC GCTGGAGGAG
    GTCAGAGAGG CGGCGGCCAA GACGTTTGAG CAGCTGCACT CCACCATCGG CCACCAGGCC
    CTGGAGGACA TCCTCCCGTT TTTACTGAAG CAGCTGGATG ACGAGGAGGT GTCCGAGTTT
    GCCTTGGATG GTCTGAAGCA GGTGATGGCC ATCAAGAGTC GTGTGGTGCT GCCCTACCTC
    GTGCCCAAGC TGACGACCCC GCCCGTCAAC ACCCGGGTGC TGGCTTTCCT TTCATCTGTG
    GCGGGTGATG CCCTGACACG TCATCTTGGC GTGATCCTCC CGGCGGTCAT GTTGGCCCTG
    AAGGAGAAGC TGGGGACTCC AGACGAGCAG CTGGAGATGG CCAACTGTCA GGCCGTGATC
    CTGTCCGTGG AGGACGACAC CGGGCACCGG ATCATCATCG AGGACCTGTT GGAAGCCACC
    CGCAGCCCTG AGGTGGGCAT GAGGCAGGCC GCGGCCATCA TCCTCAACAT CTACTGCTCA
    CGCTCCAAGG CGGACTACAC TAGCCACCTG CGGAGCCTGG TCTCAGGCCT GATCCGCCTC
    TTCAACGACT CCAGCCCCGT GGTTCTGGAG GAGAGCTGGG ACGCCCTGAA TGCCATCACC
    AAGAAACTGG ATGCAGGCAA CCAGCTGGCC CTCATTGAGG AACTGCACAA GGAAATCCGG
    TTCATAGGAA ATGAGAGCAG AGGCGAGCAC GTGCCAGGCT TCTGCCTCCC GAAGAAGGGG
    GTAACGTCCA TCCTTCCGGT GCTGCGGGAG GGGGTCCTGA CTGGCAGCCC GGAACAGAAG
    GAGGAAGCTG CCAAAGCGTT AGGCTTGGTG ATCCGCCTGA CCTCGGCGGA CGCCCTGCGG
    CCATCCGTGG TCAACATCAC TGGCCCTCTG ATTCGCATCC TGGGGGACCG GTTCAGCTGG
    AACGTGAAGG CGGCTCTGCT CGAGACACTC AGCCTCTTGC TGGCCAAGGT CGGGATCGCC
    CTGAAGCCCT TCCTGCCCCA GCTGCAGACC ACCTTCACCA AAGCCCTGCA GGACTCTAAC
    CGCGGCGTGC GCCTGAAGGC CGCCGATGCG CTAGGAAAGC TCATTTCCAT CCACATCAAG
    GTGGATCCTC TTTTCATGGA GCTGCTGAAT GGCATCCGGG TCATGGAGGA CCCAGGCGTC
    AGGGACACCA TGCTGCAGGC CCTGAGGTTT GTGATTCAGG GAGCGGGGGC CAAAGTGGAT
    GTGGTCATCC GGAAAAACAT CGTCTCGCTC CTGCTGAGCA TGCTGGGACA CGACGAGGAC
    AACACGCGCA TCTCCTCGGC CGGGTGCCTG GGGGAGCTAT GTGCCTTTCT GACGGAAGAG
    GAGCTCAGCA CTGTTCTTCA GCAGTACTTG CTGGCGGATG TGTCTGGCAT TGACTGGATG
    GTTCGGCATG GGCGGAGCCT GGCTCTCTCC GTGGCTGTGA GCGTGGCTCC TGGCAGACTC
    TGCGTAGGCA AGTATGGCAG CGACGTCCAG GACATGATCC TCAGCAATGC CATGGCAGAC
    AGGATCCCCA TCGCCGTGAG TGGGGTCCGG GGCATGGGCT TCCTGATGAA GCACCACATC
    GAGACCGGAG GAGGACAGTT GCCGGCCAAA CTCTCCAGCC TGTTCATTAA GTGTCTGCAG
    AACCCATCCA GTGACATCAG GTTGGTGGCT GAGAAGATGA TCTGGTGGGC AAACAAGGAC
    CCGCTGCCTG CCCTGGACCC CCAGGCCATC AAGCCCATCC TGAAGGCCCT CCTGGACAAC
    ACCAAGGATA AAAACACCGT CGTCAGGGCC TACAGCGACC AGGCGATTGT CAACCTCCTC
    AAGATGAGGC AGGGAGAGGA GGTGTTTCAG TCCCTCTGCA AGATCCTGGA TGTGGCCAGC
    TTGGAAGTGC TGAATGAATG CAACCGGAGG TCTCTGAAGA AGCTGGCCGG CCAGGCTGAC
    TCCACAGAGC AGGTGGACGA CACCATCCTG ACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09078
    Clone ID Related Accession (Same CDS sequence) XM_002694522.4 , XM_616264.8
    Accession Version XM_616264.8 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8016bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product translational activator GCN1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_616264.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCGGCGG ACACGCAGGT TTCTGAGACA CTAAAGCGTT TTGCAGGGAA GGTGACGACA 
    GCCAGTGTGA AGGAACGGAG AGAAATCCTC AGTGAGCTGG GGAAATGTGT TGCCGGAAAA
    GATCTGCCGG AGGGAGCTGT GAAAGGCCTC TGCAAACTCT TCTGCTTGAC CCTGCATCGA
    TACAGGGATG CAGCCTCACG CCGGGCCCTG CAGGCAGCCA TCCAGCAGCT GGCTGAGGCC
    CAGCCTGAAG CCACTGCCAA GAACCTTCTG CACTCTCTGC AGTCCTCGGG CATCGGCTCC
    AAAGCGGGTG TCCCCAGTAA GAGCAGCGGC TCGGCTGCCT TGCTGGCCCT GTCCTGGACT
    GGCCTACTGG TGCGCATCGT CTTTCCCTCG AGAGCCAAGC GGCAAGGAGA CATCTGGAAC
    AAACTGGTGG AGGTGCAGTG CCTGCTCCTG CTGGAGGTGC TGGGTGGCTC CCACAAGCAC
    GCCGTGGACA GTGCCGTGAA GAAGCTCACC AAGCTGTGGA GAGAGAACCC CGGGCTGGTG
    GAGCAGTACT TGTCAGCCAT TCTCAGCCTG GAGCCCAACC AGAGCTACGC AGGCATGCTG
    GGGCTGCTGG TGCAGTTCTG CACGAGCCAC AAGGAGATGG ACGTGGCCAA CCAGCACAAG
    AGTGCCCTGC TGGACTTTTA CGTGAAGAAC ATCCTGATGA GCAAAGTGAA GCCTCAGAAG
    TATCTGTTGG ATAACTGTGC CCCTTTGCTC CGGTACGTGT CCCACTCGGA GTTTAAGGAT
    CTGGTCCTGC CCACCATACA GAAGTCCTTA CTGAGGAGTC CAGAGAATGT CATTGAAACT
    ATCTCCAGTC TGCTGGCATC CGTGACTCTG GATCTCAGCC AGTATGCCCT GGACATTGGG
    AAAGGACTGG CTGGTCAGCT GAAGTCCAAC AGCCCTCGCT TGATGGATGA AGCCGTGCTG
    GCGCTGCGGA ACCTGGCCCG CCAGTGCAGT GACTCCTCGG CGATGGAAGC CCTGGCCAGG
    CACCTGTTCG CTATCCTCGG AGGCTCGGAG GGGAAGCTAA CTATTGTAGC CCAGAAGATG
    AGCGTTCTCT CAGGGATCGG GAGTGTCAGT CATCACGTGG TGTCGGGGCC TTCTAGTCAA
    GTCCTGAGCG GGACCGTGGC TGAGCTCTTC ATCCCGTTCC TCCAGCAAGA AGTTCATGAA
    GGCACCTTGG TGCACGCTGT CTCTGTCTTG GCTCTCTGGT GTCACCGCTT CACCACGGAA
    GTACCCAAGA AGCTCACCGA ATGGTTCAAG AAAGCCTTCA GCCTTAAAAC CTCCACCTCT
    GCCGTGAGGC ACGCCTACCT GCAGTGCATG TTGGTCTGCT TTCGAGGCGA CACACTGCTG
    CAGGCCCTGG ACTTGCTGCC CTTGCTCATC CAGACAGTGG AGAAGGCGGC CTCCCAAGGC
    ACTCAGGTTC CCGTTGTCAC CGAAGGGGTC GCCGCTGCCT TGCTGCTCAC GAAGCTCTCA
    ATGGCCGACT CGCAGGCTGA GGCCAAACTG AGTGGTTTCT GGCAGCTCAT TTTGGATGAG
    AAGAAGCAGG TTTTCACTTC CGAGAAATTC CTCCTCATGG CTTCAGAGGA TGCCCTGTGT
    ACCGTGCTGC ACCTGACAGA GAGACTGTTT CTTGACCATC CGCACCGACT CACAGGCAAC
    AAAGTTCAGC AGTACCACCG GGCCCTGGCG GCCGTGCTCC TGAGCCGCCC CTGGCACGTG
    CGCAGGCAGG CCCAGCAGAC GGCCCGGAAG CTGCTGTCCT CTCTCGGGGG CTTCAAGCTG
    GCTTACGGAC TGCTGGAGGA GCTGAAGACC GTCCTCAGTT CTCACAAGGT GCTGCCCTTC
    GAGGCTCTGG TGACCGACAC TGGGGAGGTG ACTGAGGCGG GCAAGGCCTA CGTGCCCCCG
    CGGGTCCTGC AGGAGGCTCT CTGTGTCATC TCCGGGGTGC CAGGGCTGGA GGGTGGCATC
    ACCGGCATGG AACAGCTGGC ACAGGAGATG CTGATCATCT CTCACCACCC ATCCTTAGTG
    GCTGTGCAGT CTGGCCTCTG GCCAGCACTT CTCGCCAGGA TGAAGATCGA TCCGGAAGCC
    TTCATTAGTA GGCACTTGGA CCAGATCATC CCCAGGATCA CCACCCAGAA CCCCTTGAAC
    CAGTCTTCCA TGAACGCCAT GGGCTCCCTC TCGATCCTGT CCCCAGACCG GGTCCTCCCG
    CAGCTCATCA GCACCATCAC CACCTCCGTG CAGAACCCAG CACTGTGCCA GGTGACGCGG
    GAGGAGTTTG CTATCATGCA GACCCCTGCA GGGGAGTTGT ACGACAAGTC CATCATTCAG
    AGTGCCCAGC AGGACAGCAT AAAAAAGGCC AACATGAAGC GAGAGAACAA AGCTTACTCC
    TTCAAGGAGC AGATCATCGA GTTGGAGCTG AAGGAGGAGA TAAAGAAGAA GAAAGGCATA
    AAAGAGGAGG TGCAGCTGAC CAGCAAACAG AAGGAGATGC TGCAGGCCCA GCTGGACAAG
    GAGGCGCAGA TCCGGAGGCG GCTGCAGGAG CTGGACAGTG AGCTGGAGGC GGCTCTGGGG
    CTGCTGGACA CCATCCTGGC CAAGAACCCG TCCGGCCTGA CCCAGTACAT CCCGGTCCTG
    GTCGACTCTT TCCTGCCCTT GCTGAAGTCT CCCCTGGCTG CTCCCAGGAT CAAGACCCCT
    TTCCTGTCCT TGGCTGCCTG TGTCATGCCC CCGAGGCTCA AGGCTTTGGG CACTTTGGTG
    AGCCATGTGA CTCTGCGCCT GCTGAAGCCG GAATGTGCCC TGGACGAGTC ATGGTGCCAG
    GAAGAGCTGT CGGTGGCCGT GAAGAGAGCA GTGACCCTGC TGCACAGTCA CACCATCACC
    AGCAGGGCAG GCAAGGGCGA GCCAGACGCT GCGCCCCTGT CCGCGCCAGC CTTCTCCTTG
    GTTTTCCCGT TTCTGAAGAT GGTGCTGACT GAGATGCCCC ACCACAGTGA GGAGGAGGAG
    GAAAGAATGG CCCAGATTCT TCAGATCCTC ACAGTCCACG CCCAGCTGCG GGCCTCCCCA
    AGCAACCCAC CTGGGCGTGT GGACGAGAAC GGCCCGGAGT TGCTGCCTCG CGTGGCCATG
    CTGCGCCTTC TGACCTGGGT GATTGGGACG GGCTCTCCCC GACTTCAGGT TCTGGCTTCA
    GAGGCCCTGA CCACCCTGTG TGCCAGCAGC AGTGGCGAGG ATGGCTGTGC CTTTGCAGAG
    CAGGAGGAGG TGGACGTGCT GCTCTGCGCC CTGCTGTCCC CCTGTGCCAA CGTGCGGGAC
    ACTGTACTCC GGGGCCTGTT GGAACTCCAC ATGGTGTTGC CGGCGCCTGA CACCGATGAG
    AAGAATGGCC TGAACCTTCT CCGCAGGCTC TGGGTGGTTA AGTTCGACAA GGAGGAAGAG
    ATCCGAAAGT TGGCCGAGAG GCTCTGGTCG ACCATGGGCC TAGATCTGCA GCCAGACCTC
    TGCTCCTTGC TCATCGACGA CGTCATCTAT CACGAGGCAG CTGTGAGGCA GGCCGGGGCG
    GAAGCCCTCT CCCAGGCGGT GGCGCGTTAC CAGCGGCAGG CGGCTGAGGT CATGGGCAGG
    CTCATGGAGA TCTACCAGGA GAAGCTCTAT CGGCCGCCCC CGGTGCTGGA TGCTCTGGGA
    CGAGTTATCT CCGAATCTCC TCCAGACCAG TGGGAAGCCA GGTGTGGCTT GGCTTTGGCC
    CTGAACAAGC TCTCCCAGTG TTTGGACAGC TCTCAGGTGA AGCCACTCTT TCAATTTTTT
    GTCCCCGACG CCCTCAACGA CCGAAACCCA GATGTCCGGA AGTGCATGTT GGATGCAGCT
    CTTGCGACAC TCAACACACA TGGAAAGGAG AACGTCAACT CGCTGCTGCC GGTGTTCGAG
    GAGTTCCTGA AGAACGCGCC GAACGATGCC AGCTACGATG CCGTGCGGCA GAGCGTGGTG
    GTCCTCATGG GCTCTCTAGC CAAGCACCTG GACAAGAGTG ACCCCAAAGT GAAGCCGATT
    GTCGCCAAGC TCATTGCTGC CCTCGCCACC CCCTCCCAGC AGGTCCAGGA ATCCGTGGCC
    AACTGCTTGC CACCCCTCGT CCCTGCCATA AAGGAAGACG CGGGGGGTAT GATCCAGAGG
    CTGATGCAGC AGCTGCTGGA GTCAGACAAG TACGCCGAGC GCAAAGGGGC TGCGTACGGG
    CTGGCGGGCC TGGTGAAGGG CCTGGGCATC CTGTCGCTGA AGCAGCAGGA GATGATGGCC
    GCGCTGACCG ATGCCATTCA GGACAAGAAG AACTTCCGCC GCCGGGAGGG AGCCCTCTTT
    GCCTTCGAGA TGCTCTGCAC CATGCTGGGG AAGCTCTTTG AGCCGTATGT GGTCCACGTG
    CTGCCCCATC TGCTGCTCTG CTTTGGGGAC GGGAACCAGT ATGTGCGGGA GGCTGCAGAT
    GACTGTGCCA AGGCTGTGAT GAGCAACCTG AGTGCTCATG GGGTGAAGCT GGTGCTCCCC
    TCCTTGTTGG CTGCCCTGGA GGAGGAATCC TGGAGAACCA AAGCCGGGTC GGTGGAGTTG
    CTCGGGGCCA TGGCCTACTG TGCTCCCAAG CAGCTGTCGT CCTGCCTACC CAACATTGTG
    CCCAAGCTCA CAGAGGTGCT CACCGACTCC CACGTCAAAG TGCAGAAGGC TGGGCAGCAG
    GCGCTCAGGC AGATCGGCTC TGTCATCAGG AACCCAGAGA TCCTGGCCAT CGCCCCGGTA
    CTGCTGGACG CCCTGACAGA CCCCTCCCGG AAGACCCAGA AGTGCTTGCA GACCTTGCTG
    GACACCAAGT TTGTCCACTT CATCGATGCA CCGTCCCTGG CCCTCATCAT GCCCATCGTC
    CAGAGAGCCT TCCAGGACCG TTCCACGGAC ACACGGAAGA TGGCCGCCCA GATCATTGGC
    AACATGTACT CCCTGACAGA CCAGAAGGAC TTGGCTCCTT ACCTGCCAAG CGTGACGCCT
    GGTCTGAAAG CCTCTCTCCT GGATCCTGTG CCTGAGGTAC GGACAGTGTC TGCAAAGGCT
    CTTGGGGCCA TGGTCAAGGG CATGGGGGAG TCATGCTTTG AGGACTTGCT GCCGTGGCTG
    ATGGAGACAC TGACCTACGA GCAGAGCTCT GTGGATCGCT CAGGCGCCGC GCAGGGGTTG
    GCTGAGGTCA TGGCCGGCTT GGGGGTGGAG AAGTTGGAGA AGCTGATGCC AGAAATTGTG
    GCTACAGCCA GCAAAGTGGA CATTGCCCCC CACGTCCGAG ACGGCTACAT CATGATGTTC
    AACTACCTGC CCATCACCTT CGGGGACAAA TTCACTCCTT ATGTGGGGCC CATCATCCCC
    TGTATCCTCA AAGCTCTTGC TGATGAGAAC GAGTTTGTGC GTGATACTGC CCTGCGTGCA
    GGCCAGCGGG TCATCTCCAT GTATGCTGAG ACGGCCATTG CCCTGCTGCT GCCCCAGCTG
    GAGCAAGGCC TCTTTGATGA CCTCTGGAGA ATAAGGTTCA GCTCTGTTCA GCTCCTTGGC
    GATCTCCTGT TCCACATCTC AGGAGTCACT GGGAAGATGA CCACAGAAAC TGCCTCAGAG
    GATGACAACT TTGGAACCGC GCAGTCCAAC AAGGCGATTA TCACCGCCCT GGGGGTAGAT
    CGGCGGAACC GGGTGTTGGC GGGGCTGTAC ATGGGCCGCT CGGACACCCA GCTGGTGGTG
    CGGCAGGCCT CCCTGCACGT CTGGAAGATT GTGGTCTCTA ACACCCCCCG CACCTTGCGT
    GAGATCCTTC CCACCCTTTT TGGGCTCCTG CTGGGTTTCC TGGCCAGCAC ATGCGCGGAT
    AAGAGAACAA TCGCAGCCAG GACGCTGGGA GATCTCGTCC GGAAGTTAGG GGAGAAAATC
    CTGCCGGAGA TCATCCCCAT CCTGGAGGAA GGCCTGAGAT CTCCCAAGAG TGACGAGAGG
    CAGGGCGTGT GCATCGGGCT GAGCGAGATC ATGAAGTCCA CCAGCCGGGA TGCGGTGCTG
    TATTTCTCCG AGTCCCTCGT GCCCACGGCG AGGAAGGCTT TGTGTGACCC GCTGGAGGAG
    GTCAGAGAGG CGGCGGCCAA GACGTTTGAG CAGCTGCACT CCACCATCGG CCACCAGGCC
    CTGGAGGACA TCCTCCCGTT TTTACTGAAG CAGCTGGATG ACGAGGAGGT GTCCGAGTTT
    GCCTTGGATG GTCTGAAGCA GGTGATGGCC ATCAAGAGTC GTGTGGTGCT GCCCTACCTC
    GTGCCCAAGC TGACGACCCC GCCCGTCAAC ACCCGGGTGC TGGCTTTCCT TTCATCTGTG
    GCGGGTGATG CCCTGACACG TCATCTTGGC GTGATCCTCC CGGCGGTCAT GTTGGCCCTG
    AAGGAGAAGC TGGGGACTCC AGACGAGCAG CTGGAGATGG CCAACTGTCA GGCCGTGATC
    CTGTCCGTGG AGGACGACAC CGGGCACCGG ATCATCATCG AGGACCTGTT GGAAGCCACC
    CGCAGCCCTG AGGTGGGCAT GAGGCAGGCC GCGGCCATCA TCCTCAACAT CTACTGCTCA
    CGCTCCAAGG CGGACTACAC TAGCCACCTG CGGAGCCTGG TCTCAGGCCT GATCCGCCTC
    TTCAACGACT CCAGCCCCGT GGTTCTGGAG GAGAGCTGGG ACGCCCTGAA TGCCATCACC
    AAGAAACTGG ATGCAGGCAA CCAGCTGGCC CTCATTGAGG AACTGCACAA GGAAATCCGG
    TTCATAGGAA ATGAGAGCAG AGGCGAGCAC GTGCCAGGCT TCTGCCTCCC GAAGAAGGGG
    GTAACGTCCA TCCTTCCGGT GCTGCGGGAG GGGGTCCTGA CTGGCAGCCC GGAACAGAAG
    GAGGAAGCTG CCAAAGCGTT AGGCTTGGTG ATCCGCCTGA CCTCGGCGGA CGCCCTGCGG
    CCATCCGTGG TCAACATCAC TGGCCCTCTG ATTCGCATCC TGGGGGACCG GTTCAGCTGG
    AACGTGAAGG CGGCTCTGCT CGAGACACTC AGCCTCTTGC TGGCCAAGGT CGGGATCGCC
    CTGAAGCCCT TCCTGCCCCA GCTGCAGACC ACCTTCACCA AAGCCCTGCA GGACTCTAAC
    CGCGGCGTGC GCCTGAAGGC CGCCGATGCG CTAGGAAAGC TCATTTCCAT CCACATCAAG
    GTGGATCCTC TTTTCATGGA GCTGCTGAAT GGCATCCGGG TCATGGAGGA CCCAGGCGTC
    AGGGACACCA TGCTGCAGGC CCTGAGGTTT GTGATTCAGG GAGCGGGGGC CAAAGTGGAT
    GTGGTCATCC GGAAAAACAT CGTCTCGCTC CTGCTGAGCA TGCTGGGACA CGACGAGGAC
    AACACGCGCA TCTCCTCGGC CGGGTGCCTG GGGGAGCTAT GTGCCTTTCT GACGGAAGAG
    GAGCTCAGCA CTGTTCTTCA GCAGTACTTG CTGGCGGATG TGTCTGGCAT TGACTGGATG
    GTTCGGCATG GGCGGAGCCT GGCTCTCTCC GTGGCTGTGA GCGTGGCTCC TGGCAGACTC
    TGCGTAGGCA AGTATGGCAG CGACGTCCAG GACATGATCC TCAGCAATGC CATGGCAGAC
    AGGATCCCCA TCGCCGTGAG TGGGGTCCGG GGCATGGGCT TCCTGATGAA GCACCACATC
    GAGACCGGAG GAGGACAGTT GCCGGCCAAA CTCTCCAGCC TGTTCATTAA GTGTCTGCAG
    AACCCATCCA GTGACATCAG GTTGGTGGCT GAGAAGATGA TCTGGTGGGC AAACAAGGAC
    CCGCTGCCTG CCCTGGACCC CCAGGCCATC AAGCCCATCC TGAAGGCCCT CCTGGACAAC
    ACCAAGGATA AAAACACCGT CGTCAGGGCC TACAGCGACC AGGCGATTGT CAACCTCCTC
    AAGATGAGGC AGGGAGAGGA GGTGTTTCAG TCCCTCTGCA AGATCCTGGA TGTGGCCAGC
    TTGGAAGTGC TGAATGAATG CAACCGGAGG TCTCTGAAGA AGCTGGCCGG CCAGGCTGAC
    TCCACAGAGC AGGTGGACGA CACCATCCTG ACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_616264.3
    CDS27..8042
    Translation

    Target ORF information:

    RefSeq Version XM_616264.8
    Organism Bos taurus(cattle)
    Definition Bos taurus GCN1, eIF2 alpha kinase activator homolog (GCN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_616264.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCGGCGG ACACGCAGGT TTCTGAGACA CTAAAGCGTT TTGCAGGGAA GGTGACGACA 
    GCCAGTGTGA AGGAACGGAG AGAAATCCTC AGTGAGCTGG GGAAATGTGT TGCCGGAAAA
    GATCTGCCGG AGGGAGCTGT GAAAGGCCTC TGCAAACTCT TCTGCTTGAC CCTGCATCGA
    TACAGGGATG CAGCCTCACG CCGGGCCCTG CAGGCAGCCA TCCAGCAGCT GGCTGAGGCC
    CAGCCTGAAG CCACTGCCAA GAACCTTCTG CACTCTCTGC AGTCCTCGGG CATCGGCTCC
    AAAGCGGGTG TCCCCAGTAA GAGCAGCGGC TCGGCTGCCT TGCTGGCCCT GTCCTGGACT
    GGCCTACTGG TGCGCATCGT CTTTCCCTCG AGAGCCAAGC GGCAAGGAGA CATCTGGAAC
    AAACTGGTGG AGGTGCAGTG CCTGCTCCTG CTGGAGGTGC TGGGTGGCTC CCACAAGCAC
    GCCGTGGACA GTGCCGTGAA GAAGCTCACC AAGCTGTGGA GAGAGAACCC CGGGCTGGTG
    GAGCAGTACT TGTCAGCCAT TCTCAGCCTG GAGCCCAACC AGAGCTACGC AGGCATGCTG
    GGGCTGCTGG TGCAGTTCTG CACGAGCCAC AAGGAGATGG ACGTGGCCAA CCAGCACAAG
    AGTGCCCTGC TGGACTTTTA CGTGAAGAAC ATCCTGATGA GCAAAGTGAA GCCTCAGAAG
    TATCTGTTGG ATAACTGTGC CCCTTTGCTC CGGTACGTGT CCCACTCGGA GTTTAAGGAT
    CTGGTCCTGC CCACCATACA GAAGTCCTTA CTGAGGAGTC CAGAGAATGT CATTGAAACT
    ATCTCCAGTC TGCTGGCATC CGTGACTCTG GATCTCAGCC AGTATGCCCT GGACATTGGG
    AAAGGACTGG CTGGTCAGCT GAAGTCCAAC AGCCCTCGCT TGATGGATGA AGCCGTGCTG
    GCGCTGCGGA ACCTGGCCCG CCAGTGCAGT GACTCCTCGG CGATGGAAGC CCTGGCCAGG
    CACCTGTTCG CTATCCTCGG AGGCTCGGAG GGGAAGCTAA CTATTGTAGC CCAGAAGATG
    AGCGTTCTCT CAGGGATCGG GAGTGTCAGT CATCACGTGG TGTCGGGGCC TTCTAGTCAA
    GTCCTGAGCG GGACCGTGGC TGAGCTCTTC ATCCCGTTCC TCCAGCAAGA AGTTCATGAA
    GGCACCTTGG TGCACGCTGT CTCTGTCTTG GCTCTCTGGT GTCACCGCTT CACCACGGAA
    GTACCCAAGA AGCTCACCGA ATGGTTCAAG AAAGCCTTCA GCCTTAAAAC CTCCACCTCT
    GCCGTGAGGC ACGCCTACCT GCAGTGCATG TTGGTCTGCT TTCGAGGCGA CACACTGCTG
    CAGGCCCTGG ACTTGCTGCC CTTGCTCATC CAGACAGTGG AGAAGGCGGC CTCCCAAGGC
    ACTCAGGTTC CCGTTGTCAC CGAAGGGGTC GCCGCTGCCT TGCTGCTCAC GAAGCTCTCA
    ATGGCCGACT CGCAGGCTGA GGCCAAACTG AGTGGTTTCT GGCAGCTCAT TTTGGATGAG
    AAGAAGCAGG TTTTCACTTC CGAGAAATTC CTCCTCATGG CTTCAGAGGA TGCCCTGTGT
    ACCGTGCTGC ACCTGACAGA GAGACTGTTT CTTGACCATC CGCACCGACT CACAGGCAAC
    AAAGTTCAGC AGTACCACCG GGCCCTGGCG GCCGTGCTCC TGAGCCGCCC CTGGCACGTG
    CGCAGGCAGG CCCAGCAGAC GGCCCGGAAG CTGCTGTCCT CTCTCGGGGG CTTCAAGCTG
    GCTTACGGAC TGCTGGAGGA GCTGAAGACC GTCCTCAGTT CTCACAAGGT GCTGCCCTTC
    GAGGCTCTGG TGACCGACAC TGGGGAGGTG ACTGAGGCGG GCAAGGCCTA CGTGCCCCCG
    CGGGTCCTGC AGGAGGCTCT CTGTGTCATC TCCGGGGTGC CAGGGCTGGA GGGTGGCATC
    ACCGGCATGG AACAGCTGGC ACAGGAGATG CTGATCATCT CTCACCACCC ATCCTTAGTG
    GCTGTGCAGT CTGGCCTCTG GCCAGCACTT CTCGCCAGGA TGAAGATCGA TCCGGAAGCC
    TTCATTAGTA GGCACTTGGA CCAGATCATC CCCAGGATCA CCACCCAGAA CCCCTTGAAC
    CAGTCTTCCA TGAACGCCAT GGGCTCCCTC TCGATCCTGT CCCCAGACCG GGTCCTCCCG
    CAGCTCATCA GCACCATCAC CACCTCCGTG CAGAACCCAG CACTGTGCCA GGTGACGCGG
    GAGGAGTTTG CTATCATGCA GACCCCTGCA GGGGAGTTGT ACGACAAGTC CATCATTCAG
    AGTGCCCAGC AGGACAGCAT AAAAAAGGCC AACATGAAGC GAGAGAACAA AGCTTACTCC
    TTCAAGGAGC AGATCATCGA GTTGGAGCTG AAGGAGGAGA TAAAGAAGAA GAAAGGCATA
    AAAGAGGAGG TGCAGCTGAC CAGCAAACAG AAGGAGATGC TGCAGGCCCA GCTGGACAAG
    GAGGCGCAGA TCCGGAGGCG GCTGCAGGAG CTGGACAGTG AGCTGGAGGC GGCTCTGGGG
    CTGCTGGACA CCATCCTGGC CAAGAACCCG TCCGGCCTGA CCCAGTACAT CCCGGTCCTG
    GTCGACTCTT TCCTGCCCTT GCTGAAGTCT CCCCTGGCTG CTCCCAGGAT CAAGACCCCT
    TTCCTGTCCT TGGCTGCCTG TGTCATGCCC CCGAGGCTCA AGGCTTTGGG CACTTTGGTG
    AGCCATGTGA CTCTGCGCCT GCTGAAGCCG GAATGTGCCC TGGACGAGTC ATGGTGCCAG
    GAAGAGCTGT CGGTGGCCGT GAAGAGAGCA GTGACCCTGC TGCACAGTCA CACCATCACC
    AGCAGGGCAG GCAAGGGCGA GCCAGACGCT GCGCCCCTGT CCGCGCCAGC CTTCTCCTTG
    GTTTTCCCGT TTCTGAAGAT GGTGCTGACT GAGATGCCCC ACCACAGTGA GGAGGAGGAG
    GAAAGAATGG CCCAGATTCT TCAGATCCTC ACAGTCCACG CCCAGCTGCG GGCCTCCCCA
    AGCAACCCAC CTGGGCGTGT GGACGAGAAC GGCCCGGAGT TGCTGCCTCG CGTGGCCATG
    CTGCGCCTTC TGACCTGGGT GATTGGGACG GGCTCTCCCC GACTTCAGGT TCTGGCTTCA
    GAGGCCCTGA CCACCCTGTG TGCCAGCAGC AGTGGCGAGG ATGGCTGTGC CTTTGCAGAG
    CAGGAGGAGG TGGACGTGCT GCTCTGCGCC CTGCTGTCCC CCTGTGCCAA CGTGCGGGAC
    ACTGTACTCC GGGGCCTGTT GGAACTCCAC ATGGTGTTGC CGGCGCCTGA CACCGATGAG
    AAGAATGGCC TGAACCTTCT CCGCAGGCTC TGGGTGGTTA AGTTCGACAA GGAGGAAGAG
    ATCCGAAAGT TGGCCGAGAG GCTCTGGTCG ACCATGGGCC TAGATCTGCA GCCAGACCTC
    TGCTCCTTGC TCATCGACGA CGTCATCTAT CACGAGGCAG CTGTGAGGCA GGCCGGGGCG
    GAAGCCCTCT CCCAGGCGGT GGCGCGTTAC CAGCGGCAGG CGGCTGAGGT CATGGGCAGG
    CTCATGGAGA TCTACCAGGA GAAGCTCTAT CGGCCGCCCC CGGTGCTGGA TGCTCTGGGA
    CGAGTTATCT CCGAATCTCC TCCAGACCAG TGGGAAGCCA GGTGTGGCTT GGCTTTGGCC
    CTGAACAAGC TCTCCCAGTG TTTGGACAGC TCTCAGGTGA AGCCACTCTT TCAATTTTTT
    GTCCCCGACG CCCTCAACGA CCGAAACCCA GATGTCCGGA AGTGCATGTT GGATGCAGCT
    CTTGCGACAC TCAACACACA TGGAAAGGAG AACGTCAACT CGCTGCTGCC GGTGTTCGAG
    GAGTTCCTGA AGAACGCGCC GAACGATGCC AGCTACGATG CCGTGCGGCA GAGCGTGGTG
    GTCCTCATGG GCTCTCTAGC CAAGCACCTG GACAAGAGTG ACCCCAAAGT GAAGCCGATT
    GTCGCCAAGC TCATTGCTGC CCTCGCCACC CCCTCCCAGC AGGTCCAGGA ATCCGTGGCC
    AACTGCTTGC CACCCCTCGT CCCTGCCATA AAGGAAGACG CGGGGGGTAT GATCCAGAGG
    CTGATGCAGC AGCTGCTGGA GTCAGACAAG TACGCCGAGC GCAAAGGGGC TGCGTACGGG
    CTGGCGGGCC TGGTGAAGGG CCTGGGCATC CTGTCGCTGA AGCAGCAGGA GATGATGGCC
    GCGCTGACCG ATGCCATTCA GGACAAGAAG AACTTCCGCC GCCGGGAGGG AGCCCTCTTT
    GCCTTCGAGA TGCTCTGCAC CATGCTGGGG AAGCTCTTTG AGCCGTATGT GGTCCACGTG
    CTGCCCCATC TGCTGCTCTG CTTTGGGGAC GGGAACCAGT ATGTGCGGGA GGCTGCAGAT
    GACTGTGCCA AGGCTGTGAT GAGCAACCTG AGTGCTCATG GGGTGAAGCT GGTGCTCCCC
    TCCTTGTTGG CTGCCCTGGA GGAGGAATCC TGGAGAACCA AAGCCGGGTC GGTGGAGTTG
    CTCGGGGCCA TGGCCTACTG TGCTCCCAAG CAGCTGTCGT CCTGCCTACC CAACATTGTG
    CCCAAGCTCA CAGAGGTGCT CACCGACTCC CACGTCAAAG TGCAGAAGGC TGGGCAGCAG
    GCGCTCAGGC AGATCGGCTC TGTCATCAGG AACCCAGAGA TCCTGGCCAT CGCCCCGGTA
    CTGCTGGACG CCCTGACAGA CCCCTCCCGG AAGACCCAGA AGTGCTTGCA GACCTTGCTG
    GACACCAAGT TTGTCCACTT CATCGATGCA CCGTCCCTGG CCCTCATCAT GCCCATCGTC
    CAGAGAGCCT TCCAGGACCG TTCCACGGAC ACACGGAAGA TGGCCGCCCA GATCATTGGC
    AACATGTACT CCCTGACAGA CCAGAAGGAC TTGGCTCCTT ACCTGCCAAG CGTGACGCCT
    GGTCTGAAAG CCTCTCTCCT GGATCCTGTG CCTGAGGTAC GGACAGTGTC TGCAAAGGCT
    CTTGGGGCCA TGGTCAAGGG CATGGGGGAG TCATGCTTTG AGGACTTGCT GCCGTGGCTG
    ATGGAGACAC TGACCTACGA GCAGAGCTCT GTGGATCGCT CAGGCGCCGC GCAGGGGTTG
    GCTGAGGTCA TGGCCGGCTT GGGGGTGGAG AAGTTGGAGA AGCTGATGCC AGAAATTGTG
    GCTACAGCCA GCAAAGTGGA CATTGCCCCC CACGTCCGAG ACGGCTACAT CATGATGTTC
    AACTACCTGC CCATCACCTT CGGGGACAAA TTCACTCCTT ATGTGGGGCC CATCATCCCC
    TGTATCCTCA AAGCTCTTGC TGATGAGAAC GAGTTTGTGC GTGATACTGC CCTGCGTGCA
    GGCCAGCGGG TCATCTCCAT GTATGCTGAG ACGGCCATTG CCCTGCTGCT GCCCCAGCTG
    GAGCAAGGCC TCTTTGATGA CCTCTGGAGA ATAAGGTTCA GCTCTGTTCA GCTCCTTGGC
    GATCTCCTGT TCCACATCTC AGGAGTCACT GGGAAGATGA CCACAGAAAC TGCCTCAGAG
    GATGACAACT TTGGAACCGC GCAGTCCAAC AAGGCGATTA TCACCGCCCT GGGGGTAGAT
    CGGCGGAACC GGGTGTTGGC GGGGCTGTAC ATGGGCCGCT CGGACACCCA GCTGGTGGTG
    CGGCAGGCCT CCCTGCACGT CTGGAAGATT GTGGTCTCTA ACACCCCCCG CACCTTGCGT
    GAGATCCTTC CCACCCTTTT TGGGCTCCTG CTGGGTTTCC TGGCCAGCAC ATGCGCGGAT
    AAGAGAACAA TCGCAGCCAG GACGCTGGGA GATCTCGTCC GGAAGTTAGG GGAGAAAATC
    CTGCCGGAGA TCATCCCCAT CCTGGAGGAA GGCCTGAGAT CTCCCAAGAG TGACGAGAGG
    CAGGGCGTGT GCATCGGGCT GAGCGAGATC ATGAAGTCCA CCAGCCGGGA TGCGGTGCTG
    TATTTCTCCG AGTCCCTCGT GCCCACGGCG AGGAAGGCTT TGTGTGACCC GCTGGAGGAG
    GTCAGAGAGG CGGCGGCCAA GACGTTTGAG CAGCTGCACT CCACCATCGG CCACCAGGCC
    CTGGAGGACA TCCTCCCGTT TTTACTGAAG CAGCTGGATG ACGAGGAGGT GTCCGAGTTT
    GCCTTGGATG GTCTGAAGCA GGTGATGGCC ATCAAGAGTC GTGTGGTGCT GCCCTACCTC
    GTGCCCAAGC TGACGACCCC GCCCGTCAAC ACCCGGGTGC TGGCTTTCCT TTCATCTGTG
    GCGGGTGATG CCCTGACACG TCATCTTGGC GTGATCCTCC CGGCGGTCAT GTTGGCCCTG
    AAGGAGAAGC TGGGGACTCC AGACGAGCAG CTGGAGATGG CCAACTGTCA GGCCGTGATC
    CTGTCCGTGG AGGACGACAC CGGGCACCGG ATCATCATCG AGGACCTGTT GGAAGCCACC
    CGCAGCCCTG AGGTGGGCAT GAGGCAGGCC GCGGCCATCA TCCTCAACAT CTACTGCTCA
    CGCTCCAAGG CGGACTACAC TAGCCACCTG CGGAGCCTGG TCTCAGGCCT GATCCGCCTC
    TTCAACGACT CCAGCCCCGT GGTTCTGGAG GAGAGCTGGG ACGCCCTGAA TGCCATCACC
    AAGAAACTGG ATGCAGGCAA CCAGCTGGCC CTCATTGAGG AACTGCACAA GGAAATCCGG
    TTCATAGGAA ATGAGAGCAG AGGCGAGCAC GTGCCAGGCT TCTGCCTCCC GAAGAAGGGG
    GTAACGTCCA TCCTTCCGGT GCTGCGGGAG GGGGTCCTGA CTGGCAGCCC GGAACAGAAG
    GAGGAAGCTG CCAAAGCGTT AGGCTTGGTG ATCCGCCTGA CCTCGGCGGA CGCCCTGCGG
    CCATCCGTGG TCAACATCAC TGGCCCTCTG ATTCGCATCC TGGGGGACCG GTTCAGCTGG
    AACGTGAAGG CGGCTCTGCT CGAGACACTC AGCCTCTTGC TGGCCAAGGT CGGGATCGCC
    CTGAAGCCCT TCCTGCCCCA GCTGCAGACC ACCTTCACCA AAGCCCTGCA GGACTCTAAC
    CGCGGCGTGC GCCTGAAGGC CGCCGATGCG CTAGGAAAGC TCATTTCCAT CCACATCAAG
    GTGGATCCTC TTTTCATGGA GCTGCTGAAT GGCATCCGGG TCATGGAGGA CCCAGGCGTC
    AGGGACACCA TGCTGCAGGC CCTGAGGTTT GTGATTCAGG GAGCGGGGGC CAAAGTGGAT
    GTGGTCATCC GGAAAAACAT CGTCTCGCTC CTGCTGAGCA TGCTGGGACA CGACGAGGAC
    AACACGCGCA TCTCCTCGGC CGGGTGCCTG GGGGAGCTAT GTGCCTTTCT GACGGAAGAG
    GAGCTCAGCA CTGTTCTTCA GCAGTACTTG CTGGCGGATG TGTCTGGCAT TGACTGGATG
    GTTCGGCATG GGCGGAGCCT GGCTCTCTCC GTGGCTGTGA GCGTGGCTCC TGGCAGACTC
    TGCGTAGGCA AGTATGGCAG CGACGTCCAG GACATGATCC TCAGCAATGC CATGGCAGAC
    AGGATCCCCA TCGCCGTGAG TGGGGTCCGG GGCATGGGCT TCCTGATGAA GCACCACATC
    GAGACCGGAG GAGGACAGTT GCCGGCCAAA CTCTCCAGCC TGTTCATTAA GTGTCTGCAG
    AACCCATCCA GTGACATCAG GTTGGTGGCT GAGAAGATGA TCTGGTGGGC AAACAAGGAC
    CCGCTGCCTG CCCTGGACCC CCAGGCCATC AAGCCCATCC TGAAGGCCCT CCTGGACAAC
    ACCAAGGATA AAAACACCGT CGTCAGGGCC TACAGCGACC AGGCGATTGT CAACCTCCTC
    AAGATGAGGC AGGGAGAGGA GGTGTTTCAG TCCCTCTGCA AGATCCTGGA TGTGGCCAGC
    TTGGAAGTGC TGAATGAATG CAACCGGAGG TCTCTGAAGA AGCTGGCCGG CCAGGCTGAC
    TCCACAGAGC AGGTGGACGA CACCATCCTG ACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224654
    Clone ID Related Accession (Same CDS sequence) XM_002694522.5
    Accession Version XM_002694522.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8016bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eIF-2-alpha kinase activator GCN1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002694522.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCGGCGG ACACGCAGGT TTCTGAGACA CTAAAGCGTT TTGCAGGGAA GGTGACGACA 
    GCCAGTGTGA AGGAACGGAG AGAAATCCTC AGTGAGCTGG GGAAATGTGT TGCCGGAAAA
    GATCTGCCGG AGGGAGCTGT GAAAGGCCTC TGCAAACTCT TCTGCTTGAC CCTGCATCGA
    TACAGGGATG CAGCCTCACG CCGGGCCCTG CAGGCAGCCA TCCAGCAGCT GGCTGAGGCC
    CAGCCTGAAG CCACTGCCAA GAACCTTCTG CACTCTCTGC AGTCCTCGGG CATCGGCTCC
    AAAGCGGGTG TCCCCAGTAA GAGCAGCGGC TCGGCTGCCT TGCTGGCCCT GTCCTGGACT
    GGCCTACTGG TGCGCATCGT CTTTCCCTCG AGAGCCAAGC GGCAAGGAGA CATCTGGAAC
    AAACTGGTGG AGGTGCAGTG CCTGCTCCTG CTGGAGGTGC TGGGTGGCTC CCACAAGCAC
    GCCGTGGACA GTGCCGTGAA GAAGCTCACC AAGCTGTGGA GAGAGAACCC CGGGCTGGTG
    GAGCAGTACT TGTCAGCCAT TCTCAGCCTG GAGCCCAACC AGAGCTACGC AGGCATGCTG
    GGGCTGCTGG TGCAGTTCTG CACGAGCCAC AAGGAGATGG ACGTGGCCAA CCAGCACAAG
    AGTGCCCTGC TGGACTTTTA CGTGAAGAAC ATCCTGATGA GCAAAGTGAA GCCTCAGAAG
    TATCTGTTGG ATAACTGTGC CCCTTTGCTC CGGTACGTGT CCCACTCGGA GTTTAAGGAT
    CTGGTCCTGC CCACCATACA GAAGTCCTTA CTGAGGAGTC CAGAGAATGT CATTGAAACT
    ATCTCCAGTC TGCTGGCATC CGTGACTCTG GATCTCAGCC AGTATGCCCT GGACATTGGG
    AAAGGACTGG CTGGTCAGCT GAAGTCCAAC AGCCCTCGCT TGATGGATGA AGCCGTGCTG
    GCGCTGCGGA ACCTGGCCCG CCAGTGCAGT GACTCCTCGG CGATGGAAGC CCTGGCCAGG
    CACCTGTTCG CTATCCTCGG AGGCTCGGAG GGGAAGCTAA CTATTGTAGC CCAGAAGATG
    AGCGTTCTCT CAGGGATCGG GAGTGTCAGT CATCACGTGG TGTCGGGGCC TTCTAGTCAA
    GTCCTGAGCG GGACCGTGGC TGAGCTCTTC ATCCCGTTCC TCCAGCAAGA AGTTCATGAA
    GGCACCTTGG TGCACGCTGT CTCTGTCTTG GCTCTCTGGT GTCACCGCTT CACCACGGAA
    GTACCCAAGA AGCTCACCGA ATGGTTCAAG AAAGCCTTCA GCCTTAAAAC CTCCACCTCT
    GCCGTGAGGC ACGCCTACCT GCAGTGCATG TTGGTCTGCT TTCGAGGCGA CACACTGCTG
    CAGGCCCTGG ACTTGCTGCC CTTGCTCATC CAGACAGTGG AGAAGGCGGC CTCCCAAGGC
    ACTCAGGTTC CCGTTGTCAC CGAAGGGGTC GCCGCTGCCT TGCTGCTCAC GAAGCTCTCA
    ATGGCCGACT CGCAGGCTGA GGCCAAACTG AGTGGTTTCT GGCAGCTCAT TTTGGATGAG
    AAGAAGCAGG TTTTCACTTC CGAGAAATTC CTCCTCATGG CTTCAGAGGA TGCCCTGTGT
    ACCGTGCTGC ACCTGACAGA GAGACTGTTT CTTGACCATC TGCACCGACT CACAGGCAAC
    AAAGTTCAGC AGTACCACCG GGCCCTGGCG GCCGTGCTCC TGAGCCGCCC CTGGCACGTG
    CGCAGGCAGG CCCAGCAGAC GGCCCGGAAG CTGCTGTCCT CTCTCGGGGG CTTCAAGCTG
    GCTTACGGAC TGCTGGAGGA GCTGAAGACC GTCCTCAGTT CTCACAAGGT GCTGCCCTTC
    GAGGCTCTGG TGACTGACAC TGGGGAGGTG ACTGAGGCGG GCAAGGCCTA CGTGCCCCCG
    CGGGTCCTGC AGGAGGCTCT CTGTGTCATC TCCGGGGTGC CAGGGCTGGA GGGTGGCATC
    ACCGGCATGG AACAGCTGGC ACAGGAGATG CTGATCATCT CTCACCACCC ATCCTTAGTG
    GCTGTGCAGT CTGGCCTCTG GCCAGCACTT CTCGCCAGGA TGAAGATCGA TCCGGAAGCC
    TTCATTAGTA GGCACTTGGA CCAGATCATC CCCAGGATCA CCACCCAGAA CCCCTTGAAC
    CAGTCTTCCA TGAACGCCAT GGGCTCCCTC TCGATCCTGT CCCCAGACCG GGTCCTCCCG
    CAGCTCATCA GCACCATCAC CACCTCCGTG CAGAACCCAG CACTGTGCCA GGTGACGCGG
    GAGGAGTTTG CTATCATGCA GACCCCTGCA GGGGAGTTGT ACGACAAGTC CATCATTCAG
    AGTGCCCAGC AGGACAGCAT AAAAAAGGCC AACATGAAGC GAGAGAACAA AGCTTACTCC
    TTCAAGGAGC AGATCATCGA GTTGGAGCTG AAGGAGGAGA TAAAGAAGAA GAAAGGCATA
    AAAGAGGAGG TGCAGCTGAC CAGCAAACAG AAGGAGATGC TGCAGGCCCA GCTGGACAAG
    GAGGCGCAGA TCCGGAGGCG GCTGCAGGAG CTGGACAGTG AGCTGGAGGC GGCTCTGGGG
    CTGCTGGACA CCATCCTGGC CAAGAACCCG TCCGGCCTGA CCCAGTACAT CCCGGTCCTG
    GTCGACTCTT TCCTGCCCTT GCTGAAGTCT CCCCTGGCTG CTCCCAGGAT CAAGACCCCT
    TTCCTGTCCT TGGCTGCCTG TGTCATGCCC CCGAGGCTCA AGGCTTTGGG CACTTTGGTG
    AGCCATGTGA CTCTGCGCCT GCTGAAGCCG GAATGTGCCC TGGACGAGTC ATGGTGCCAG
    GAAGAGCTGT CGGTGGCCGT GAAGAGAGCA GTGACCCTGC TGCACAGTCA CACCATCACC
    AGCAGGGCAG GCAAGGGCGA GCCAGACGCT GCGCCCCTGT CCGCGCCAGC CTTCTCCTTG
    GTTTTCCCGT TTCTGAAGAT GGTGCTGACT GAGATGCCCC ACCACAGTGA GGAGGAGGAG
    GAAAGAATGG CCCAGATTCT TCAGATCCTC ACAGTCCACG CCCAGCTGCG GGCCTCCCCA
    AGCAACCCAC CTGGGCGTGT GGACGAGAAC GGCCCGGAGT TGCTGCCTCG CGTGGCCATG
    CTGCGCCTTC TGACCTGGGT GATTGGGACG GGCTCTCCCC GACTTCAGGT TCTGGCTTCA
    GAGGCCCTGA CCACCCTGTG TGCCAGCAGC AGTGGCGAGG ATGGCTGTGC CTTTGCAGAG
    CAGGAGGAGG TGGACGTGCT GCTCTGCGCC CTGCTGTCCC CCTGTGCCAA CGTGCGGGAC
    ACTGTACTCC GGGGCCTGTT GGAACTCCAC ATGGTGTTGC CGGCGCCTGA CACCGATGAG
    AAGAATGGCC TGAACCTTCT CCGCAGGCTC TGGGTGGTTA AGTTCGACAA GGAGGAAGAG
    ATCCGAAAGT TGGCCGAGAG GCTCTGGTCG ACCATGGGCC TAGATCTGCA GCCAGACCTC
    TGCTCCTTGC TCATCGACGA CGTCATCTAT CACGAGGCAG CTGTGAGGCA GGCCGGGGCG
    GAAGCCCTCT CCCAGGCGGT GGCGCGTTAC CAGCGGCAGG CGGCTGAGGT CATGGGCAGG
    CTCATGGAGA TCTACCAGGA GAAGCTCTAT CGGCCGCCCC CGGTGCTGGA TGCTCTGGGA
    CGAGTTATCT CCGAATCTCC TCCAGACCAG TGGGAAGCCA GGTGTGGCTT GGCTTTGGCC
    CTGAACAAGC TCTCCCAGTG TTTGGACAGC TCTCAGGTGA AGCCACTCTT TCAATTTTTT
    GTCCCCGACG CCCTCAACGA CCGAAACCCA GATGTCCGGA AGTGCATGTT GGATGCAGCT
    CTTGCGACAC TCAACACACA TGGAAAGGAG AACGTCAACT CGCTGCTGCC GGTGTTCGAG
    GAGTTCCTGA AGAACGCGCC GAACGATGCC AGCTACGATG CCGTGCGGCA GAGCGTGGTG
    GTCCTCATGG GCTCTCTAGC CAAGCACCTG GACAAGAGTG ACCCCAAAGT GAAGCCGATT
    GTCGCCAAGC TCATTGCTGC CCTCGCCACC CCCTCCCAGC AGGTCCAGGA ATCCGTGGCC
    AACTGCTTGC CACCCCTCGT CCCTGCCATA AAGGAAGACG CGGGGGGTAT GATCCAGAGG
    CTGATGCAGC AGCTGCTGGA GTCAGACAAG TACGCCGAGC GCAAAGGGGC TGCGTACGGG
    CTGGCGGGCC TGGTGAAGGG CCTGGGCATC CTGTCGCTGA AGCAGCAGGA GATGATGGCC
    GCGCTGACCG ATGCCATTCA GGACAAGAAG AACTTCCGCC GCCGGGAGGG AGCCCTCTTT
    GCCTTCGAGA TGCTCTGCAC CATGCTGGGG AAGCTCTTTG AGCCGTATGT GGTCCACGTG
    CTGCCCCATC TGCTGCTCTG CTTTGGGGAC GGGAACCAGT ATGTGCGGGA GGCTGCAGAT
    GACTGTGCCA AGGCTGTGAT GAGCAACCTG AGTGCTCATG GGGTGAAGCT GGTGCTCCCC
    TCCTTGTTGG CTGCCCTGGA GGAGGAATCC TGGAGAACCA AAGCCGGGTC GGTGGAGTTG
    CTCGGGGCCA TGGCCTACTG TGCTCCCAAG CAGCTGTCGT CCTGCCTACC CAACATTGTG
    CCCAAGCTCA CAGAGGTGCT CACCGACTCC CACGTCAAAG TGCAGAAGGC TGGGCAGCAG
    GCGCTCAGGC AGATCGGCTC TGTCATCAGG AACCCAGAGA TCCTGGCCAT CGCCCCGGTA
    CTGCTGGACG CCCTGACAGA CCCCTCCCGG AAGACCCAGA AGTGCTTGCA GACCTTGCTG
    GACACCAAGT TTGTCCACTT CATCGATGCA CCGTCCCTGG CCCTCATCAT GCCCATCGTC
    CAGAGAGCCT TCCAGGACCG TTCCACGGAC ACACGGAAGA TGGCCGCCCA GATCATTGGC
    AACATGTACT CCCTGACAGA CCAGAAGGAC TTGGCTCCTT ACCTGCCAAG CGTGACGCCT
    GGTCTGAAAG CCTCTCTCCT GGATCCTGTG CCTGAGGTAC GGACAGTGTC TGCAAAGGCT
    CTTGGGGCCA TGGTCAAGGG CATGGGGGAG TCATGCTTTG AGGACTTGCT GCCGTGGCTG
    ATGGAGACAC TGACCTACGA GCAGAGCTCT GTGGATCGCT CAGGCGCCGC GCAGGGGTTG
    GCTGAGGTCA TGGCCGGCTT GGGGGTGGAG AAGTTGGAGA AGCTGATGCC AGAAATTGTG
    GCTACAGCCA GCAAAGTGGA CATTGCCCCC CACGTCCGAG ACGGCTACAT CATGATGTTC
    AACTACCTGC CCATCACCTT CGGGGACAAA TTCACTCCTT ATGTGGGGCC CATCATCCCC
    TGTATCCTCA AAGCTCTTGC TGATGAGAAC GAGTTTGTGC GTGATACTGC CCTGCGTGCA
    GGCCAGCGGG TCATCTCCAT GTATGCTGAG ACGGCCATTG CCCTGCTGCT GCCCCAGCTG
    GAGCAAGGCC TCTTTGATGA CCTCTGGAGA ATAAGGTTCA GCTCTGTTCA GCTCCTTGGC
    GATCTCCTGT TCCACATCTC AGGAGTCACT GGGAAGATGA CCACAGAAAC TGCCTCAGAG
    GATGACAACT TTGGAACCGC GCAGTCCAAC AAGGCGATTA TCACCGCCCT GGGGGTAGAT
    CGGCGGAACC GGGTGTTGGC GGGGCTGTAC ATGGGCCGCT CGGACACCCA GCTGGTGGTG
    CGGCAGGCCT CCCTGCACGT CTGGAAGATT GTGGTCTCTA ACACCCCCCG CACCTTGCGT
    GAGATCCTTC CCACCCTTTT TGGGCTCCTG CTGGGTTTCC TGGCCAGCAC ATGCGCGGAT
    AAGAGAACAA TCGCAGCCAG GACGCTGGGA GATCTCGTCC GGAAGTTAGG GGAGAAAATC
    CTGCCGGAGA TCATCCCCAT CCTGGAGGAA GGCCTGAGAT CTCCCAAGAG TGACGAGAGG
    CAGGGCGTGT GCATCGGGCT GAGCGAGATC ATGAAGTCCA CCAGCCGGGA TGCGGTGCTG
    TATTTCTCCG AGTCCCTCGT GCCCACGGCG AGGAAGGCTT TGTGTGACCC GCTGGAGGAG
    GTCAGAGAGG CGGCGGCCAA GACGTTTGAG CAGCTGCACT CCACCATCGG CCACCAGGCC
    CTGGAGGACA TCCTCCCGTT TTTACTGAAG CAGCTGGATG ACGAGGAGGT GTCCGAGTTT
    GCCTTGGATG GTCTGAAGCA GGTGATGGCC ATCAAGAGTC GTGTGGTGCT GCCCTACCTC
    GTGCCCAAGC TGACGACCCC GCCCGTCAAC ACCCGGGTGC TGGCTTTCCT TTCATCTGTG
    GCGGGTGATG CCCTGACACG TCATCTTGGC GTGATCCTCC CGGCGGTCAT GTTGGCCCTG
    AAGGAGAAGC TGGGGACTCC AGACGAGCAG CTGGAGATGG CCAACTGTCA GGCCGTGATC
    CTGTCCGTGG AGGACGACAC CGGGCACCGG ATCATCATCG AGGACCTGTT GGAAGCCACC
    CGCAGCCCTG AGGTGGGCAT GAGGCAGGCC GCGGCCATCA TCCTCAACAT CTACTGCTCA
    CGCTCCAAGG CGGACTACAC TAGCCACCTG CGGAGCCTGG TCTCAGGCCT GATCCGCCTC
    TTCAACGACT CCAGCCCCGT GGTTCTGGAG GAGAGCTGGG ACGCCCTGAA TGCCATCACC
    AAGAAACTGG ATGCAGGCAA CCAGCTGGCC CTCATTGAGG AACTGCACAA GGAAATCCGG
    TTCATAGGAA ATGAGAGCAG AGGCGAGCAC GTGCCAGGCT TCTGCCTCCC GAAGAAGGGG
    GTAACGTCCA TCCTTCCGGT GCTGCGGGAG GGGGTCCTGA CTGGCAGCCC GGAACAGAAG
    GAGGAAGCTG CCAAAGCGTT AGGCTTGGTG ATCCGCCTGA CCTCGGCGGA CGCCCTGCGG
    CCATCCGTGG TCAACATCAC TGGCCCTCTG ATTCGCATCC TGGGGGACCG GTTCAGCTGG
    AACGTGAAGG CGGCTCTGCT CGAGACACTC AGCCTCTTGC TGGCCAAGGT CGGGATCGCC
    CTGAAGCCCT TCCTGCCCCA GCTGCAGACC ACCTTCACCA AAGCCCTGCA GGACTCTAAC
    CGCGGCGTGC GCCTGAAGGC CGCCGATGCG CTAGGAAAGC TCATTTCCAT CCACATCAAG
    GTGGATCCTC TTTTCATGGA GCTGCTGAAT GGCATCCGGG TCATGGAGGA CCCAGGCGTC
    AGGGACACCA TGCTGCAGGC CCTGAGGTTT GTGATTCAGG GAGCGGGGGC CAAAGTGGAT
    GTGGTCATCC GGAAAAACAT CGTCTCGCTC CTGCTGAGCA TGCTGGGACA CGACGAGGAC
    AACACGCGCA TCTCCTCGGC CGGGTGCCTG GGGGAGCTAT GTGCCTTTCT GACGGAAGAG
    GAGCTCAGCA CTGTTCTTCA GCAGTACTTG CTGGCGGATG TGTCTGGCAT TGACTGGATG
    GTTCGGCATG GGCGGAGCCT GGCTCTCTCC GTGGCTGTGA GCGTGGCTCC TGGCAGACTC
    TGCGTAGGCA AGTATGGCAG CGACGTCCAG GACATGATCC TCAGCAATGC CATGGCAGAC
    AGGATCCCCA TCGCCGTGAG TGGGGTCCGG GGCATGGGCT TCCTGATGAA GCACCACATC
    GAGACCGGAG GAGGACAGTT GCCGGCCAAA CTCTCCAGCC TGTTCATTAA GTGTCTGCAG
    AACCCATCCA GTGACATCAG GTTGGTGGCT GAGAAGATGA TCTGGTGGGC AAACAAGGAC
    CCGCTGCCTG CCCTGGACCC CCAGGCCATC AAGCCCATCC TGAAGGCCCT CCTGGACAAC
    ACCAAGGATA AAAACACCGT CGTCAGGGCC TACAGCGACC AGGCGATTGT CAACCTCCTC
    AAGATGAGGC AGGGAGAGGA GGTGTTTCAG TCCCTCTGCA AGATCCTGGA TGTGGCCAGC
    TTGGAAGTGC TGAATGAATG CAACCGGAGG TCTCTGAAGA AGCTGGCCGG CCAGGCTGAC
    TCCACAGAGC AGGTGGACGA CACCATCCTG ACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002694568.2
    CDS60..8075
    Translation

    Target ORF information:

    RefSeq Version XM_002694522.5
    Organism Bos taurus(cattle)
    Definition Bos taurus GCN1, eIF2 alpha kinase activator homolog (GCN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002694522.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCGGCGG ACACGCAGGT TTCTGAGACA CTAAAGCGTT TTGCAGGGAA GGTGACGACA 
    GCCAGTGTGA AGGAACGGAG AGAAATCCTC AGTGAGCTGG GGAAATGTGT TGCCGGAAAA
    GATCTGCCGG AGGGAGCTGT GAAAGGCCTC TGCAAACTCT TCTGCTTGAC CCTGCATCGA
    TACAGGGATG CAGCCTCACG CCGGGCCCTG CAGGCAGCCA TCCAGCAGCT GGCTGAGGCC
    CAGCCTGAAG CCACTGCCAA GAACCTTCTG CACTCTCTGC AGTCCTCGGG CATCGGCTCC
    AAAGCGGGTG TCCCCAGTAA GAGCAGCGGC TCGGCTGCCT TGCTGGCCCT GTCCTGGACT
    GGCCTACTGG TGCGCATCGT CTTTCCCTCG AGAGCCAAGC GGCAAGGAGA CATCTGGAAC
    AAACTGGTGG AGGTGCAGTG CCTGCTCCTG CTGGAGGTGC TGGGTGGCTC CCACAAGCAC
    GCCGTGGACA GTGCCGTGAA GAAGCTCACC AAGCTGTGGA GAGAGAACCC CGGGCTGGTG
    GAGCAGTACT TGTCAGCCAT TCTCAGCCTG GAGCCCAACC AGAGCTACGC AGGCATGCTG
    GGGCTGCTGG TGCAGTTCTG CACGAGCCAC AAGGAGATGG ACGTGGCCAA CCAGCACAAG
    AGTGCCCTGC TGGACTTTTA CGTGAAGAAC ATCCTGATGA GCAAAGTGAA GCCTCAGAAG
    TATCTGTTGG ATAACTGTGC CCCTTTGCTC CGGTACGTGT CCCACTCGGA GTTTAAGGAT
    CTGGTCCTGC CCACCATACA GAAGTCCTTA CTGAGGAGTC CAGAGAATGT CATTGAAACT
    ATCTCCAGTC TGCTGGCATC CGTGACTCTG GATCTCAGCC AGTATGCCCT GGACATTGGG
    AAAGGACTGG CTGGTCAGCT GAAGTCCAAC AGCCCTCGCT TGATGGATGA AGCCGTGCTG
    GCGCTGCGGA ACCTGGCCCG CCAGTGCAGT GACTCCTCGG CGATGGAAGC CCTGGCCAGG
    CACCTGTTCG CTATCCTCGG AGGCTCGGAG GGGAAGCTAA CTATTGTAGC CCAGAAGATG
    AGCGTTCTCT CAGGGATCGG GAGTGTCAGT CATCACGTGG TGTCGGGGCC TTCTAGTCAA
    GTCCTGAGCG GGACCGTGGC TGAGCTCTTC ATCCCGTTCC TCCAGCAAGA AGTTCATGAA
    GGCACCTTGG TGCACGCTGT CTCTGTCTTG GCTCTCTGGT GTCACCGCTT CACCACGGAA
    GTACCCAAGA AGCTCACCGA ATGGTTCAAG AAAGCCTTCA GCCTTAAAAC CTCCACCTCT
    GCCGTGAGGC ACGCCTACCT GCAGTGCATG TTGGTCTGCT TTCGAGGCGA CACACTGCTG
    CAGGCCCTGG ACTTGCTGCC CTTGCTCATC CAGACAGTGG AGAAGGCGGC CTCCCAAGGC
    ACTCAGGTTC CCGTTGTCAC CGAAGGGGTC GCCGCTGCCT TGCTGCTCAC GAAGCTCTCA
    ATGGCCGACT CGCAGGCTGA GGCCAAACTG AGTGGTTTCT GGCAGCTCAT TTTGGATGAG
    AAGAAGCAGG TTTTCACTTC CGAGAAATTC CTCCTCATGG CTTCAGAGGA TGCCCTGTGT
    ACCGTGCTGC ACCTGACAGA GAGACTGTTT CTTGACCATC TGCACCGACT CACAGGCAAC
    AAAGTTCAGC AGTACCACCG GGCCCTGGCG GCCGTGCTCC TGAGCCGCCC CTGGCACGTG
    CGCAGGCAGG CCCAGCAGAC GGCCCGGAAG CTGCTGTCCT CTCTCGGGGG CTTCAAGCTG
    GCTTACGGAC TGCTGGAGGA GCTGAAGACC GTCCTCAGTT CTCACAAGGT GCTGCCCTTC
    GAGGCTCTGG TGACTGACAC TGGGGAGGTG ACTGAGGCGG GCAAGGCCTA CGTGCCCCCG
    CGGGTCCTGC AGGAGGCTCT CTGTGTCATC TCCGGGGTGC CAGGGCTGGA GGGTGGCATC
    ACCGGCATGG AACAGCTGGC ACAGGAGATG CTGATCATCT CTCACCACCC ATCCTTAGTG
    GCTGTGCAGT CTGGCCTCTG GCCAGCACTT CTCGCCAGGA TGAAGATCGA TCCGGAAGCC
    TTCATTAGTA GGCACTTGGA CCAGATCATC CCCAGGATCA CCACCCAGAA CCCCTTGAAC
    CAGTCTTCCA TGAACGCCAT GGGCTCCCTC TCGATCCTGT CCCCAGACCG GGTCCTCCCG
    CAGCTCATCA GCACCATCAC CACCTCCGTG CAGAACCCAG CACTGTGCCA GGTGACGCGG
    GAGGAGTTTG CTATCATGCA GACCCCTGCA GGGGAGTTGT ACGACAAGTC CATCATTCAG
    AGTGCCCAGC AGGACAGCAT AAAAAAGGCC AACATGAAGC GAGAGAACAA AGCTTACTCC
    TTCAAGGAGC AGATCATCGA GTTGGAGCTG AAGGAGGAGA TAAAGAAGAA GAAAGGCATA
    AAAGAGGAGG TGCAGCTGAC CAGCAAACAG AAGGAGATGC TGCAGGCCCA GCTGGACAAG
    GAGGCGCAGA TCCGGAGGCG GCTGCAGGAG CTGGACAGTG AGCTGGAGGC GGCTCTGGGG
    CTGCTGGACA CCATCCTGGC CAAGAACCCG TCCGGCCTGA CCCAGTACAT CCCGGTCCTG
    GTCGACTCTT TCCTGCCCTT GCTGAAGTCT CCCCTGGCTG CTCCCAGGAT CAAGACCCCT
    TTCCTGTCCT TGGCTGCCTG TGTCATGCCC CCGAGGCTCA AGGCTTTGGG CACTTTGGTG
    AGCCATGTGA CTCTGCGCCT GCTGAAGCCG GAATGTGCCC TGGACGAGTC ATGGTGCCAG
    GAAGAGCTGT CGGTGGCCGT GAAGAGAGCA GTGACCCTGC TGCACAGTCA CACCATCACC
    AGCAGGGCAG GCAAGGGCGA GCCAGACGCT GCGCCCCTGT CCGCGCCAGC CTTCTCCTTG
    GTTTTCCCGT TTCTGAAGAT GGTGCTGACT GAGATGCCCC ACCACAGTGA GGAGGAGGAG
    GAAAGAATGG CCCAGATTCT TCAGATCCTC ACAGTCCACG CCCAGCTGCG GGCCTCCCCA
    AGCAACCCAC CTGGGCGTGT GGACGAGAAC GGCCCGGAGT TGCTGCCTCG CGTGGCCATG
    CTGCGCCTTC TGACCTGGGT GATTGGGACG GGCTCTCCCC GACTTCAGGT TCTGGCTTCA
    GAGGCCCTGA CCACCCTGTG TGCCAGCAGC AGTGGCGAGG ATGGCTGTGC CTTTGCAGAG
    CAGGAGGAGG TGGACGTGCT GCTCTGCGCC CTGCTGTCCC CCTGTGCCAA CGTGCGGGAC
    ACTGTACTCC GGGGCCTGTT GGAACTCCAC ATGGTGTTGC CGGCGCCTGA CACCGATGAG
    AAGAATGGCC TGAACCTTCT CCGCAGGCTC TGGGTGGTTA AGTTCGACAA GGAGGAAGAG
    ATCCGAAAGT TGGCCGAGAG GCTCTGGTCG ACCATGGGCC TAGATCTGCA GCCAGACCTC
    TGCTCCTTGC TCATCGACGA CGTCATCTAT CACGAGGCAG CTGTGAGGCA GGCCGGGGCG
    GAAGCCCTCT CCCAGGCGGT GGCGCGTTAC CAGCGGCAGG CGGCTGAGGT CATGGGCAGG
    CTCATGGAGA TCTACCAGGA GAAGCTCTAT CGGCCGCCCC CGGTGCTGGA TGCTCTGGGA
    CGAGTTATCT CCGAATCTCC TCCAGACCAG TGGGAAGCCA GGTGTGGCTT GGCTTTGGCC
    CTGAACAAGC TCTCCCAGTG TTTGGACAGC TCTCAGGTGA AGCCACTCTT TCAATTTTTT
    GTCCCCGACG CCCTCAACGA CCGAAACCCA GATGTCCGGA AGTGCATGTT GGATGCAGCT
    CTTGCGACAC TCAACACACA TGGAAAGGAG AACGTCAACT CGCTGCTGCC GGTGTTCGAG
    GAGTTCCTGA AGAACGCGCC GAACGATGCC AGCTACGATG CCGTGCGGCA GAGCGTGGTG
    GTCCTCATGG GCTCTCTAGC CAAGCACCTG GACAAGAGTG ACCCCAAAGT GAAGCCGATT
    GTCGCCAAGC TCATTGCTGC CCTCGCCACC CCCTCCCAGC AGGTCCAGGA ATCCGTGGCC
    AACTGCTTGC CACCCCTCGT CCCTGCCATA AAGGAAGACG CGGGGGGTAT GATCCAGAGG
    CTGATGCAGC AGCTGCTGGA GTCAGACAAG TACGCCGAGC GCAAAGGGGC TGCGTACGGG
    CTGGCGGGCC TGGTGAAGGG CCTGGGCATC CTGTCGCTGA AGCAGCAGGA GATGATGGCC
    GCGCTGACCG ATGCCATTCA GGACAAGAAG AACTTCCGCC GCCGGGAGGG AGCCCTCTTT
    GCCTTCGAGA TGCTCTGCAC CATGCTGGGG AAGCTCTTTG AGCCGTATGT GGTCCACGTG
    CTGCCCCATC TGCTGCTCTG CTTTGGGGAC GGGAACCAGT ATGTGCGGGA GGCTGCAGAT
    GACTGTGCCA AGGCTGTGAT GAGCAACCTG AGTGCTCATG GGGTGAAGCT GGTGCTCCCC
    TCCTTGTTGG CTGCCCTGGA GGAGGAATCC TGGAGAACCA AAGCCGGGTC GGTGGAGTTG
    CTCGGGGCCA TGGCCTACTG TGCTCCCAAG CAGCTGTCGT CCTGCCTACC CAACATTGTG
    CCCAAGCTCA CAGAGGTGCT CACCGACTCC CACGTCAAAG TGCAGAAGGC TGGGCAGCAG
    GCGCTCAGGC AGATCGGCTC TGTCATCAGG AACCCAGAGA TCCTGGCCAT CGCCCCGGTA
    CTGCTGGACG CCCTGACAGA CCCCTCCCGG AAGACCCAGA AGTGCTTGCA GACCTTGCTG
    GACACCAAGT TTGTCCACTT CATCGATGCA CCGTCCCTGG CCCTCATCAT GCCCATCGTC
    CAGAGAGCCT TCCAGGACCG TTCCACGGAC ACACGGAAGA TGGCCGCCCA GATCATTGGC
    AACATGTACT CCCTGACAGA CCAGAAGGAC TTGGCTCCTT ACCTGCCAAG CGTGACGCCT
    GGTCTGAAAG CCTCTCTCCT GGATCCTGTG CCTGAGGTAC GGACAGTGTC TGCAAAGGCT
    CTTGGGGCCA TGGTCAAGGG CATGGGGGAG TCATGCTTTG AGGACTTGCT GCCGTGGCTG
    ATGGAGACAC TGACCTACGA GCAGAGCTCT GTGGATCGCT CAGGCGCCGC GCAGGGGTTG
    GCTGAGGTCA TGGCCGGCTT GGGGGTGGAG AAGTTGGAGA AGCTGATGCC AGAAATTGTG
    GCTACAGCCA GCAAAGTGGA CATTGCCCCC CACGTCCGAG ACGGCTACAT CATGATGTTC
    AACTACCTGC CCATCACCTT CGGGGACAAA TTCACTCCTT ATGTGGGGCC CATCATCCCC
    TGTATCCTCA AAGCTCTTGC TGATGAGAAC GAGTTTGTGC GTGATACTGC CCTGCGTGCA
    GGCCAGCGGG TCATCTCCAT GTATGCTGAG ACGGCCATTG CCCTGCTGCT GCCCCAGCTG
    GAGCAAGGCC TCTTTGATGA CCTCTGGAGA ATAAGGTTCA GCTCTGTTCA GCTCCTTGGC
    GATCTCCTGT TCCACATCTC AGGAGTCACT GGGAAGATGA CCACAGAAAC TGCCTCAGAG
    GATGACAACT TTGGAACCGC GCAGTCCAAC AAGGCGATTA TCACCGCCCT GGGGGTAGAT
    CGGCGGAACC GGGTGTTGGC GGGGCTGTAC ATGGGCCGCT CGGACACCCA GCTGGTGGTG
    CGGCAGGCCT CCCTGCACGT CTGGAAGATT GTGGTCTCTA ACACCCCCCG CACCTTGCGT
    GAGATCCTTC CCACCCTTTT TGGGCTCCTG CTGGGTTTCC TGGCCAGCAC ATGCGCGGAT
    AAGAGAACAA TCGCAGCCAG GACGCTGGGA GATCTCGTCC GGAAGTTAGG GGAGAAAATC
    CTGCCGGAGA TCATCCCCAT CCTGGAGGAA GGCCTGAGAT CTCCCAAGAG TGACGAGAGG
    CAGGGCGTGT GCATCGGGCT GAGCGAGATC ATGAAGTCCA CCAGCCGGGA TGCGGTGCTG
    TATTTCTCCG AGTCCCTCGT GCCCACGGCG AGGAAGGCTT TGTGTGACCC GCTGGAGGAG
    GTCAGAGAGG CGGCGGCCAA GACGTTTGAG CAGCTGCACT CCACCATCGG CCACCAGGCC
    CTGGAGGACA TCCTCCCGTT TTTACTGAAG CAGCTGGATG ACGAGGAGGT GTCCGAGTTT
    GCCTTGGATG GTCTGAAGCA GGTGATGGCC ATCAAGAGTC GTGTGGTGCT GCCCTACCTC
    GTGCCCAAGC TGACGACCCC GCCCGTCAAC ACCCGGGTGC TGGCTTTCCT TTCATCTGTG
    GCGGGTGATG CCCTGACACG TCATCTTGGC GTGATCCTCC CGGCGGTCAT GTTGGCCCTG
    AAGGAGAAGC TGGGGACTCC AGACGAGCAG CTGGAGATGG CCAACTGTCA GGCCGTGATC
    CTGTCCGTGG AGGACGACAC CGGGCACCGG ATCATCATCG AGGACCTGTT GGAAGCCACC
    CGCAGCCCTG AGGTGGGCAT GAGGCAGGCC GCGGCCATCA TCCTCAACAT CTACTGCTCA
    CGCTCCAAGG CGGACTACAC TAGCCACCTG CGGAGCCTGG TCTCAGGCCT GATCCGCCTC
    TTCAACGACT CCAGCCCCGT GGTTCTGGAG GAGAGCTGGG ACGCCCTGAA TGCCATCACC
    AAGAAACTGG ATGCAGGCAA CCAGCTGGCC CTCATTGAGG AACTGCACAA GGAAATCCGG
    TTCATAGGAA ATGAGAGCAG AGGCGAGCAC GTGCCAGGCT TCTGCCTCCC GAAGAAGGGG
    GTAACGTCCA TCCTTCCGGT GCTGCGGGAG GGGGTCCTGA CTGGCAGCCC GGAACAGAAG
    GAGGAAGCTG CCAAAGCGTT AGGCTTGGTG ATCCGCCTGA CCTCGGCGGA CGCCCTGCGG
    CCATCCGTGG TCAACATCAC TGGCCCTCTG ATTCGCATCC TGGGGGACCG GTTCAGCTGG
    AACGTGAAGG CGGCTCTGCT CGAGACACTC AGCCTCTTGC TGGCCAAGGT CGGGATCGCC
    CTGAAGCCCT TCCTGCCCCA GCTGCAGACC ACCTTCACCA AAGCCCTGCA GGACTCTAAC
    CGCGGCGTGC GCCTGAAGGC CGCCGATGCG CTAGGAAAGC TCATTTCCAT CCACATCAAG
    GTGGATCCTC TTTTCATGGA GCTGCTGAAT GGCATCCGGG TCATGGAGGA CCCAGGCGTC
    AGGGACACCA TGCTGCAGGC CCTGAGGTTT GTGATTCAGG GAGCGGGGGC CAAAGTGGAT
    GTGGTCATCC GGAAAAACAT CGTCTCGCTC CTGCTGAGCA TGCTGGGACA CGACGAGGAC
    AACACGCGCA TCTCCTCGGC CGGGTGCCTG GGGGAGCTAT GTGCCTTTCT GACGGAAGAG
    GAGCTCAGCA CTGTTCTTCA GCAGTACTTG CTGGCGGATG TGTCTGGCAT TGACTGGATG
    GTTCGGCATG GGCGGAGCCT GGCTCTCTCC GTGGCTGTGA GCGTGGCTCC TGGCAGACTC
    TGCGTAGGCA AGTATGGCAG CGACGTCCAG GACATGATCC TCAGCAATGC CATGGCAGAC
    AGGATCCCCA TCGCCGTGAG TGGGGTCCGG GGCATGGGCT TCCTGATGAA GCACCACATC
    GAGACCGGAG GAGGACAGTT GCCGGCCAAA CTCTCCAGCC TGTTCATTAA GTGTCTGCAG
    AACCCATCCA GTGACATCAG GTTGGTGGCT GAGAAGATGA TCTGGTGGGC AAACAAGGAC
    CCGCTGCCTG CCCTGGACCC CCAGGCCATC AAGCCCATCC TGAAGGCCCT CCTGGACAAC
    ACCAAGGATA AAAACACCGT CGTCAGGGCC TACAGCGACC AGGCGATTGT CAACCTCCTC
    AAGATGAGGC AGGGAGAGGA GGTGTTTCAG TCCCTCTGCA AGATCCTGGA TGTGGCCAGC
    TTGGAAGTGC TGAATGAATG CAACCGGAGG TCTCTGAAGA AGCTGGCCGG CCAGGCTGAC
    TCCACAGAGC AGGTGGACGA CACCATCCTG ACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.