GCN1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	GCN1
Entrez Gene ID	416982
Full Name	GCN1, eIF2 alpha kinase activator homolog
Synonyms	GCN1L1
General protein information	Preferred Names GCN1, eIF2 alpha kinase activator homolog Names eIF-2-alpha kinase activator GCN1 translational activator GCN1 GCN1 general control of amino-acid synthesis 1-like 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015275397.1	XP_015130883.1	translational activator GCN1 isoform X1
XM_015275398.2	XP_015130884.1	eIF-2-alpha kinase activator GCN1 isoform X2
XM_015275398.1	XP_015130884.1	translational activator GCN1 isoform X2
XM_015275397.2	XP_015130883.1	eIF-2-alpha kinase activator GCN1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GCN1L1	XP_534701.2
Danio rerio (zebrafish)	gcn1l1	XP_002667084.2
Caenorhabditis elegans (roundworm)	gcn-1	NP_497506.3
Oryza sativa (rice)	Os03g0721200	NP_001051102.1
Saccharomyces cerevisiae (baker's yeast)	GCN1	NP_011320.3
Schizosaccharomyces pombe (fission yeast)	SPAC18G6.05c	NP_593669.1
Magnaporthe oryzae (rice blast fungus)	MGG_04710	XP_003710832.1
Arabidopsis thaliana (thale cress)	ILA	NP_176659.6
Pan troglodytes (chimpanzee)	GCN1L1	XP_001159608.2
Mus musculus (house mouse)	Gcn1l1	NP_766307.2
Rattus norvegicus (Norway rat)	Gcn1l1	NP_001162135.1
Eremothecium gossypii	AGOS_AFR169W	NP_985716.2
Homo sapiens (human)	GCN1L1	NP_006827.1
Bos taurus (cattle)	GCN1L1	XP_002694568.1
Gallus gallus (chicken)	GCN1L1	XP_003642271.2
Drosophila melanogaster (fruit fly)	CG17514	NP_001015316.1
Neurospora crassa	NCU05803	XP_960063.1
Xenopus tropicalis (tropical clawed frog)	gcn1l1	NP_001096529.1
	GCN1L1	XP_001087027.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001700	XP_321391.4
Kluyveromyces lactis	KLLA0F12430g	XP_455642.1

Gene Ontology

The following GCN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa42822	XM_015275397.2 Latest version!	Gallus gallus GCN1, eIF2 alpha kinase activator homolog (GCN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OGa42823	XM_015275398.2 Latest version!	Gallus gallus GCN1, eIF2 alpha kinase activator homolog (GCN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OGa42823	XM_015275398.1	Gallus gallus GCN1 eIF2 alpha kinase activator homolog (GCN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OGa42822	XM_015275397.1	Gallus gallus GCN1 eIF2 alpha kinase activator homolog (GCN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GCN1 ( XM_015275397.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015130883.1 Gallus gallus GCN1 eIF2 alpha kinase activator homolog (GCN1), transcript variant X1, mRNA.

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CloneID	OGa42822
Clone ID Related Accession (Same CDS sequence)	XM_015275397.1 , XM_015275397.2
Accession Version	XM_015275397.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	translational activator GCN1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471461.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG ACACGCAGAT ATCTGACACA CTGAAGCGGT TTGCAGTGAA GGTAACTACA 
GCCAGCGTGA AGGAGCGGAG GGAGATCCTC AGTGAGCTGG GAAAATGCAT TTCTGGGAAA 
GATCTTCCCG AGGGTGCAGT GAAAGGCCTT TGCAAACTCT TCTGCCTTAC TCTGCACCGA 
TACCGCGACG CGGCGTCGCG CAGAGCCCTG CAGTTGGCGC TTCAGCAGCT CGCGGAGTCC 
CAGCCAGATG CAACAGCAAA AAACCTTCTG CAGTCACTTC AGTCTTCGGG GATCAGTGGC 
AAGGCTGGAG CTCCCAGTAA GAGCAGTGGA TCTGCAGCCC TGCTGGCACT CTCCTGGACA 
TGCCTGCTGG TACGGACAGT CTTTCCATCA CCTGACAAGA GACAGGGAGA AACCTGGAAA 
AAACTGGTGG AGGTTCAATG TTTACTTCTT TTGGAAGTCT TGGGAGGCTC TCATAAGCAG 
GCAGTGGTTG GAGCAGTGAA GAAACTGAAC CGACTTTGGA AAGAGAACCA AGATCTGGTG 
GATCAGTACC TATCTGTCAT TCTTGGTCTT GAACCAAACC AGAGCTATGC TGCAATGCTG 
GGACTTGTGG TGCAGTTTTG CACAGCTCAG AAAGAGACTG ATATTATTAA ACGCCACAAG 
AGTGCTCTTC TGGATTTCTA TGTGAAGACC ATCCTGATGA GCAAGACAAA GCCTCAGAAG 
CATGTGCTGG ACAGCTGCTC TCCACTTCTT CGATATATCT CTCACGCTGA ATTCAAAGAC 
TTACTGTTGC CAACTCTGCA GAAATCCTTG CTACGGAGCC CTGAGAATGT AATTGAAACA 
ATCTCTTGCT TGCTCTTGTC TGTTAACCTG GATCTTAGCC AGTATGCTCT GGACATTGTG 
AAAGGACTGG CTGGTCATCT GAAATCCAAC AGTGTGCAGC TCATGGATGA AGCAGTTGTC 
GCATTAAAGA ATTTGGCTCG ACAGTGTAGT GATCCTTCAG CCGTGGAATC CCTGAGCAGA 
CACCTTTTTG CCATCTTAGG GGGCTCAGAG GGGAAGCTGA CAGTTGTTGC GCAGAAGATG 
AGCGTCCTTT CAGGGATTGG CAGCCTTAGT CACCATGTGG TTTCTGGATC ATCTAGCCAA 
GCTCTGGCTG GAACCGTGGC TGAGCTTTTC ATCCCTTTTC TGCAGCAAGA AGTCCACGAA 
GGCACGTTGG TTCATGCAGT CTCTGTCCTG GCTCTGTGGT GCGTTCGCTT CACCACTGAA 
GTCCCTAAGA AGATGGTGGA ATGGCTAAAG AAAGCATTTG GCCTTAAAAC CTCTACTTCA 
GCTGTGAGGC ATGCCTACCT GCAGTGCATG CTAGCCTCTT TCAAAGGTGA CACGTTATTA 
CAGGCAATGG ACTTGCTACC GATGCTGATC CAGACAGTAG AAAAAGCAGC ATCTCAAAGT 
ACTCAGATTC CCATGGTAAC AGAAGGAGTT GCTGCAGCTT TGTTGATCTG CAGGATGTCT 
GTAATTGAGG GACAAACTGA GTCTAAGCTG AGTGGCTTCT GGCAGCTGAT CCTAGATGAG 
AAAAAACAAA TTTTTACCTC TGAGAAGTTC TTGCAGTCTG CTTCAGAGGA AGCAATGTGC 
ACTGTGCTGC AGCTGACAGA GCGCCTCCTC TTGGACCATG CACAGCGGCT CCCCGAGAGT 
AAAATCCAGC AGTATCACCG TGCCCTTGTT GCAGTGCTGC TGAGTCGGAC CTGGCACGTT 
CGAAGACAGG CCCATCAGAC AGTTAGGAAG CTTTTGTCCT CTCTGGGAGG GTACAAACTG 
GCCTACGGGC TCTTAGAGGA GCTAAAAGTG GTTCTCAGCT CTCATAAGGT TATGCCTCAA 
GAAGTCCTGG TGATGGAGTC AGGTGAGCTG TCAGAACAAG GGAAGACTTA TATTCCTCCC 
CGTGTCCTTC ATGAGGCACT GTGTGTCATC TCAAGTGTAG CTGGGCTGGA TGTGGATTCC 
TTTGAGACAG AAAAGCTGGC TCTGGAGATG CTGCTGGTGA GCCATCATCC ATCTCTGGTG 
GCAATACAGC CAGGTCTTTG GCCAGCCCTC CTTGTCAAGA TGAAGATAGA TCCCAAAGAC 
TTTATTACTA AACACCTGAA TGACATATTA CCCAGACTCA CTGCCTACTC TCCAGACAAC 
CAGTCATCTA TGAATGCAGT GGGATCTCTC TCTCTGCTCT CACCTGGTAG AGTGCTCCCA 
CAGCTCATCA GCACCATCTC AGCATCAATG CAGAATCCAG CTCTGTGCCA GGTTACTCGA 
GAGGAATTTG CCATCATGAA GACACCGGAG GGAGAACTGT ATGATAAGTC TATAATACAG 
AGTGCTCAAC AGGATAGTAT GAAAAAGGCT AACATGAAGA GGGAGAATAA AGCCTACTCC 
TTCAAGGAAC AAATCATTGA GCTGGAGCTG AAGGAGGAGA TAAAGAAGAA GAAGGGGATA 
AAGGATGAGG TGCAGCTGAC TAGCAAGCAG AAAGAGCTGA TGCATGCACA GCTGGAAAAG 
GAATCTCAGA TAAGAAAACG ACTCAAGGAG CTTGATAATG AACTGGAAAC AGCCTTGGGA 
CTGCTGGACA CTGTTATCAA GAGAAAACCT CCTGGTCTAA CTCAGTACAT CCCTAGTCTT 
GTTGGTTCCT TTTTGCCACT TTTCAGATCG CCTTTGGCTG CTCCAAGAAT TAAGGATCCT 
TTTCTTTCCT TAGCTTCCTG TGTCATGCCT GCTCGACTTA AAACCTTTGG TACTTTAGTG 
AGCCACGTGA CCTTGCGCCT GATGAAACCA GAATGTGAGT TAGATGAATC GTGGTGTCAA 
GAAGAGCTTC CTACTGCTCT CAACAGAGTA GTAAGCTTGC TGCATGCCCA CACCATTCCT 
AGCCGAACTG GCAAAGGAGA GGCAGGTGCT GCCCCATTAT CAGCTCCAGC ATTTTCCCTA 
GTCTTTCCTC TCCTAAAGAC GGCTATGACT GAGACATCCC ATGACAGTGA AGATAAGGAG 
GAATTCCTGG TCAAGATTCT GCAGATCCTT ACAGTTCATG CTCAGCTGAG ATCATCCACA 
AACGGCCAGG CTTTGCTGGT GGATGAGAAT GGCCCGGAGT TGCTGCCCCG ACGGGACATG 
CTGCTTCTGC TGACCCGAGT GATAGCAACA GGCTCTCCAC GGTTACAGGT CTTGGCTTCC 
AATGCACTGA CCTCTGTGTG CACAAGTAGC AGTGGAGAGG ATGGGTGTGC CTATGCAGAA 
CAAGAGGAGA TTGATGTGTT ACTTCAGGCC CTGCAGTCTC CATGCATGAA TGTTCGAGAT 
GCAGCTCTCA GGGGACTGAT GGAGCTACAG ATGGTTCTAC CAACCCCAGA CAGCGATGAA 
AAGAATGGAT TAAATCTGTT GCGTCGCCTT TGGGTAGTAA AGTTTGATGT GGAAGATGAG 
ATTCAGAAGC TGGCAGAGAG GTTGTGGGAG TCCATGGGTC TGGAACTACA GCCTGACCTC 
TGTTCTCTGC TGATCAAGGA TGTCATCTAC CATGAAGAGG CAGTGCGACA AGCAGGTGCC 
GAAGCTCTTT CCAAAGCTGT AGCTCAGTAT CAGCACCAGG CAGCAGAGGT TATGAATAAA 
CTTACAGAGA TTTACCAGGA GAAGCTCTAT AGGCCACCTC CAGTTTTGGA TGCTTTGGGA 
CGAGTGATAT CTGAATCACC ACCTGACCAG TGGGAAGCAA GGTGTGGCAT TGCTTTGGCC 
CTTAACAAGC TCTCAGAGCA TCTGGACAGT TCTCAGGTGA AGCCACTCTT TCAGTTTTTT 
GTACCAGATG CTCTGAATGA TCGCAATCCT GAAGTCAGGA AGTGCATGTT AGATGCAGCT 
CTTTCAACGC TCAACACTCA TGGCAAAGAC AATGTTAACT CTCTCTTGCC AGTGTTCGAA 
GAGTTCCTGA AAAATGCTCC TAATGATGCC AGTTATGATG CTGTCAGGCA GAGTGTGGTC 
ATTCTGATGG GCTCACTGGC AAAACATCTG GACAAAAGTG ACCCTAAAGT GAAACCAATT 
GTTGGCAAAC TGATTGCAGC CTTGTCCACA CCATCTCAGC AGGTTCAAGA GTCAGTGGCA 
AGCTGTCTTC CACCACTGGT GCCTGCAATT AAGGAGGATG CAGGAGGAAT GATACAGAAG 
CTAATGCAGC TGCTTTTAGA ATCTGATAAA TATGCCGAGC GCAAAGGTGC AGCTTATGGA 
CTGGCAGGTC TGGTGAAAGG CCTTGGCATC CTTTCTCTCA AGCAGCAGGA GATGATGACC 
ACACTGACTG ATGCTATCCA AGACAAGAAG AACTTTCGTC GGCGGGAGGG GGCTCTATTT 
GCCTTTGAGA TGCTCTGCAG CATGCTTGGA AAGCTCTTTG AGCCCTATGT GGTTCATGTG 
CTACCTCACT TGTTGCTTTG TTTTGGAGAT GGGAACCAGT ATGTGCGAGA GGCTGCAGAT 
GACTGTGCCA AAGCAGTGAT GAGCAACTTG AGTACTCATG GAGTGAAGCT GGTTTTGCCA 
TCACTTCTTG CTGCACTAGA AGAGGAATCT TGGAGAACCA AAGCTGGGTC AGTGGAACTG 
CTGGGAGCTA TGGCATACTG TGCTCCCAAA CAGCTCTCCT CCTGCTTACC CAATATTGTG 
CCAAAACTTA CTGAAGTGCT CACAGATTCT CATGTGAAGG TGCAGAATGC AGGTCAGCAA 
GCTCTCAGGC AGATTGGGTC CGTCATCAGA AATCCAGAGA TCCTTGCAAT TGCTCCAGTT 
TTGTTGGATG CTCTCACTGA CCCCTCCAGG AAGACCCAAA AGTGTCTGCA GACTCTACTG 
GATACCAAAT TTGTCCACTT CATTGATGCT CCATCCTTAG CACTTATTAT GCCAATTGTT 
CAGAGAGCTT TTCAGGATCG TTCCACAGAT ACCAGGAAAA TGGCTGCCCA GATTATTGGG 
AACATGTACT CCTTGACTGA TCAGAAGGAT CTGGCTCCTT ACCTACCCAG TGTGACTCCT 
GGACTGAAGG CATCCCTGCT GGATCCTGTA CCTGAAGTGC GTACAGTATC TGCAAAGGCA 
CTGGGAGCCA TGGTGAAGGG CATGGGAGAA TCATGCTTTG AGGATTTGTT GCCATGGCTG 
ATGGAGACAC TGACATACGA GCAAAGCTCA GTGGACCGAT CTGGTGCAGC TCAAGGTCTG 
GCTGAAGTTA TGGCTGGTTT AGGGGTGGAG AAGCTGGAGA AACTTATGCC AGAGATTGTA 
GCTACTGCCA GTAAGGTGGA TATTGCTCCA CATGTACGAG ATGGTTATAT AATGATGTTT 
AACTACCTGC CAATTACTTT TGGAGACAAG TTCATTCCGT ATGTTGGTCC CATCATCCCT 
TGTATCCTTA AGGCACTGGC AGATGAGAAT GAGTTTGTGC GGGACACTGC CCTGCGTGCT 
GGCCAGAGGA TCATAAGTAT GTATGCAGAG ACAGCCATTG CACTGCTTCT GCCACAGCTT 
GAGGATGGCC TGTTTGATGA CCTGTGGAGG ATAAGGTTTA GCTCAGTTCA GCTCCTTGGA 
GATCTTTTAT TCCATATCTC AGGAGTCACT GGAAAAATGA CCACAGAAAC TGCCTCAGAA 
GATGACAACT TTGGAACAGC CCAGTCAAAC AAGGCTATTA TTAATGCTCT TGGGGTGGAG 
AGGAGGAACA GGGTGCTGGC AGGGCTGTAC ATGGGCCGCT CTGACACCCA GCTGGTAGTG 
CGTCAAGCTT CGTTACACGT TTGGAAGATT GTAGTTTCAA ACACTCCTCG CACGCTGCGT 
GAAATTTTGC CAACACTCTT TGGGCTTCTG CTGAAATTCT TGGCCAGTAC TTGTGCAGAT 
AAGCGAACGG TTGCAGCACG GACATTAGGA GACCTTGTCC GAAAGTTAGG AGAGAAGATC 
CTTCCTGAAA TCATTCCCAT TCTGGAAGAT GGCCTAAGGT CAGATAAGAA TGATGAAAGG 
CAAGGAGTCT GCATTGGGCT GAGTGAGATA ATGAAGTCTA CCAGTAGAGA TGCGGTACTG 
GTTTTCTCTG AGTCCCTAGT GCCTACAGTG AGAAAAGCAT TGTGTGACCC TCTGGAAGAA 
GTGAGAGAGG CTGCAGCTAA AACATTTGAA CAGTTGCACT CAACAATTGG GCATCAGGCT 
CTTGAAGATA TCCTTCCGTT TTTGTTGAAG CAGCTGGATA ATGAAGAAAC ATCTGACTTT 
GCTGTGGATG GTCTTAAACA AGTTATGGCT GTCAAGAGCC GTGTGGTACT GCCTTATTTG 
GTGCCAAAGC TGACTACTCC CCCTGTGAAC ACCCGTGTGC TAGCTTTCCT CTCATCAGTG 
GCAGGGGATG CTCTGACAAG GCACTTGAGT GTCATCCTGC CTGCTATGAT GTCTGCACTG 
AAAGAGAAGC TGGGGACTAG TGAAGAGCAA CTGGAAATGG CAAATTGCCA GTCTGTAATC 
CTTTCTGTGG AAGATGATGT TGGACAAAGA ATTATAACTG AGGATTTACT AGAAGCTACC 
CGCAGCCCTG ACGTGGGAAT GAGACAAGCA GCAGCTATCA TTCTGAATAT CTACTGCTCC 
AAGACAAAAG CAGACTACAC TGGTCATCTC AAGAACTTGG TTTCAGGCCT GATACGTTTA 
TTTAATGATA CCAATCCTGT AGTTTTGAAT GAAAGCTGGG ATGCACTTAA TTCCATCACC 
AAGAAATTAG ATGCTGGGAA CCAGCTTGCT CTCATTGAGG ACCTACACAA GGACATCCGG 
GTTGTAGGGA ATGAGGCCAA AGGCGAGCAT GTGCCAGGAT TTTGTATTCC TAAGAAGGGA 
GTGACTTCTA TACTTCTGGT GCTGCGTGAA GGTGTTCTAA CTGGTAATCC AGAGCAAAAG 
GAGGAAGCAG CAAAGGCCTT GGGGCTGGTT ATTAAGCTGA CTTCTGCGGA AGCTCTTAAA 
CCATCTGTAG TGAGCATTAC TGGGCCACTT ATTCGTATCT TGGGAGATCG GTTCAGCTGG 
AATGTCAAAG TGGCTCTGCT TGAAACACTA AGCCTTCTTT TAGCCAAGGT TGAGATTGCC 
CTGAAACCAT TTTTGCCTCA ACTGCAAACA ACCTTCACCA AAGCTCTGCA AGATTCAAAC 
CGTGCTGTTC GCCTTAAAGC TGCTGATGCT CTTGGTAAAC TTATTGTTAT CCATGTAAAA 
GTGGATCCTC TTTTTACTGA GCTGCTGAAT GGAATTCGTT CCAGTGATGA CTCTGCCATC 
AGGGACACGA TGCTGCAAGC ACTGCGGTTT GTAACACGAG GAGCTGGTGC AAAAGTAGAT 
GCCACAATTA GGAAGAACAT CAGCACAGTT CTTCTTGGGA TGCTGGGACA CGATGAGGAT 
GCCACCCGCA TGGCTTCAGC TGGCTGCTTA GCAGAACTGT GTGCATTTCT GTCAGAAGAG 
GAGCTCAATA CTGTTCTCCA CCAGCACTTA CTGGCAGACA TCTCTGGAAT TGACTGGATG 
GTGAGACATG GACGAAGCCT GGCCCTCTCA GTGGCTGTGA ATGTTGCTCC TTATAGGCTG 
TGTTCCCCCA AGTACTATAA CAGTGTTCTG GAGATGATCC TCAGCAATGC CACTGCTGAC 
AGGATACCCA TTGCAGTTAG TGGCATCAGA GGGATGGGCT TTCTTATGAA ATACCACATG 
GAAGAAGGAG GGAACCTGCC TCCAAAGCTT TCCAACCTCT TTATTAAGTG TCTCCAGAAC 
TCATCCAGTG ACATAAAGTT AGTTGCAGAA AAAATGATCT GGTGGGCAAA TAAAAACCAT 
CTTCCTGCAC TGGATCCTCA GACAATTAAA CCAATTCTCA AAGCGCTTCT GGACAACACA 
AAAGACAAAA ACACCAGTGT AAGAGCTTAC AGCGACCAAG CCATTGTTAA TCTTCTGAAG 
ATGAGAGTGG GTGAACAAGT GCTTGAGTCT GTTTCCAAGA TTTTAGATGC TGCCAGCTTG 
GAGCTTCTCA ATGAAAGCTG CAGGAGATCT CTTAAGAAGC TGGCTGGCCA GGCTGACTCT 
GATGAACAGA TAGATGACAC TATACTGACG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015130883.1
CDS	63..8075
Translation	MAADTQISDTLKRFAVKVTTASVKERREILSELGKCISGKDLPEGAVKGLCKLFCLTLHRYRDAASRRALQLALQQLAESQPDATAKNLLQSLQSSGISGKAGAPSKSSGSAALLALSWTCLLVRTVFPSPDKRQGETWKKLVEVQCLLLLEVLGGSHKQAVVGAVKKLNRLWKENQDLVDQYLSVILGLEPNQSYAAMLGLVVQFCTAQKETDIIKRHKSALLDFYVKTILMSKTKPQKHVLDSCSPLLRYISHAEFKDLLLPTLQKSLLRSPENVIETISCLLLSVNLDLSQYALDIVKGLAGHLKSNSVQLMDEAVVALKNLARQCSDPSAVESLSRHLFAILGGSEGKLTVVAQKMSVLSGIGSLSHHVVSGSSSQALAGTVAELFIPFLQQEVHEGTLVHAVSVLALWCVRFTTEVPKKMVEWLKKAFGLKTSTSAVRHAYLQCMLASFKGDTLLQAMDLLPMLIQTVEKAASQSTQIPMVTEGVAAALLICRMSVIEGQTESKLSGFWQLILDEKKQIFTSEKFLQSASEEAMCTVLQLTERLLLDHAQRLPESKIQQYHRALVAVLLSRTWHVRRQAHQTVRKLLSSLGGYKLAYGLLEELKVVLSSHKVMPQEVLVMESGELSEQGKTYIPPRVLHEALCVISSVAGLDVDSFETEKLALEMLLVSHHPSLVAIQPGLWPALLVKMKIDPKDFITKHLNDILPRLTAYSPDNQSSMNAVGSLSLLSPGRVLPQLISTISASMQNPALCQVTREEFAIMKTPEGELYDKSIIQSAQQDSMKKANMKRENKAYSFKEQIIELELKEEIKKKKGIKDEVQLTSKQKELMHAQLEKESQIRKRLKELDNELETALGLLDTVIKRKPPGLTQYIPSLVGSFLPLFRSPLAAPRIKDPFLSLASCVMPARLKTFGTLVSHVTLRLMKPECELDESWCQEELPTALNRVVSLLHAHTIPSRTGKGEAGAAPLSAPAFSLVFPLLKTAMTETSHDSEDKEEFLVKILQILTVHAQLRSSTNGQALLVDENGPELLPRRDMLLLLTRVIATGSPRLQVLASNALTSVCTSSSGEDGCAYAEQEEIDVLLQALQSPCMNVRDAALRGLMELQMVLPTPDSDEKNGLNLLRRLWVVKFDVEDEIQKLAERLWESMGLELQPDLCSLLIKDVIYHEEAVRQAGAEALSKAVAQYQHQAAEVMNKLTEIYQEKLYRPPPVLDALGRVISESPPDQWEARCGIALALNKLSEHLDSSQVKPLFQFFVPDALNDRNPEVRKCMLDAALSTLNTHGKDNVNSLLPVFEEFLKNAPNDASYDAVRQSVVILMGSLAKHLDKSDPKVKPIVGKLIAALSTPSQQVQESVASCLPPLVPAIKEDAGGMIQKLMQLLLESDKYAERKGAAYGLAGLVKGLGILSLKQQEMMTTLTDAIQDKKNFRRREGALFAFEMLCSMLGKLFEPYVVHVLPHLLLCFGDGNQYVREAADDCAKAVMSNLSTHGVKLVLPSLLAALEEESWRTKAGSVELLGAMAYCAPKQLSSCLPNIVPKLTEVLTDSHVKVQNAGQQALRQIGSVIRNPEILAIAPVLLDALTDPSRKTQKCLQTLLDTKFVHFIDAPSLALIMPIVQRAFQDRSTDTRKMAAQIIGNMYSLTDQKDLAPYLPSVTPGLKASLLDPVPEVRTVSAKALGAMVKGMGESCFEDLLPWLMETLTYEQSSVDRSGAAQGLAEVMAGLGVEKLEKLMPEIVATASKVDIAPHVRDGYIMMFNYLPITFGDKFIPYVGPIIPCILKALADENEFVRDTALRAGQRIISMYAETAIALLLPQLEDGLFDDLWRIRFSSVQLLGDLLFHISGVTGKMTTETASEDDNFGTAQSNKAIINALGVERRNRVLAGLYMGRSDTQLVVRQASLHVWKIVVSNTPRTLREILPTLFGLLLKFLASTCADKRTVAARTLGDLVRKLGEKILPEIIPILEDGLRSDKNDERQGVCIGLSEIMKSTSRDAVLVFSESLVPTVRKALCDPLEEVREAAAKTFEQLHSTIGHQALEDILPFLLKQLDNEETSDFAVDGLKQVMAVKSRVVLPYLVPKLTTPPVNTRVLAFLSSVAGDALTRHLSVILPAMMSALKEKLGTSEEQLEMANCQSVILSVEDDVGQRIITEDLLEATRSPDVGMRQAAAIILNIYCSKTKADYTGHLKNLVSGLIRLFNDTNPVVLNESWDALNSITKKLDAGNQLALIEDLHKDIRVVGNEAKGEHVPGFCIPKKGVTSILLVLREGVLTGNPEQKEEAAKALGLVIKLTSAEALKPSVVSITGPLIRILGDRFSWNVKVALLETLSLLLAKVEIALKPFLPQLQTTFTKALQDSNRAVRLKAADALGKLIVIHVKVDPLFTELLNGIRSSDDSAIRDTMLQALRFVTRGAGAKVDATIRKNISTVLLGMLGHDEDATRMASAGCLAELCAFLSEEELNTVLHQHLLADISGIDWMVRHGRSLALSVAVNVAPYRLCSPKYYNSVLEMILSNATADRIPIAVSGIRGMGFLMKYHMEEGGNLPPKLSNLFIKCLQNSSSDIKLVAEKMIWWANKNHLPALDPQTIKPILKALLDNTKDKNTSVRAYSDQAIVNLLKMRVGEQVLESVSKILDAASLELLNESCRRSLKKLAGQADSDEQIDDTILT

Target ORF information:

RefSeq Version	XM_015275397.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus GCN1 eIF2 alpha kinase activator homolog (GCN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015275397.1

ORF Insert Sequence:

ATGGCGGCCG ACACGCAGAT ATCTGACACA CTGAAGCGGT TTGCAGTGAA GGTAACTACA 
GCCAGCGTGA AGGAGCGGAG GGAGATCCTC AGTGAGCTGG GAAAATGCAT TTCTGGGAAA 
GATCTTCCCG AGGGTGCAGT GAAAGGCCTT TGCAAACTCT TCTGCCTTAC TCTGCACCGA 
TACCGCGACG CGGCGTCGCG CAGAGCCCTG CAGTTGGCGC TTCAGCAGCT CGCGGAGTCC 
CAGCCAGATG CAACAGCAAA AAACCTTCTG CAGTCACTTC AGTCTTCGGG GATCAGTGGC 
AAGGCTGGAG CTCCCAGTAA GAGCAGTGGA TCTGCAGCCC TGCTGGCACT CTCCTGGACA 
TGCCTGCTGG TACGGACAGT CTTTCCATCA CCTGACAAGA GACAGGGAGA AACCTGGAAA 
AAACTGGTGG AGGTTCAATG TTTACTTCTT TTGGAAGTCT TGGGAGGCTC TCATAAGCAG 
GCAGTGGTTG GAGCAGTGAA GAAACTGAAC CGACTTTGGA AAGAGAACCA AGATCTGGTG 
GATCAGTACC TATCTGTCAT TCTTGGTCTT GAACCAAACC AGAGCTATGC TGCAATGCTG 
GGACTTGTGG TGCAGTTTTG CACAGCTCAG AAAGAGACTG ATATTATTAA ACGCCACAAG 
AGTGCTCTTC TGGATTTCTA TGTGAAGACC ATCCTGATGA GCAAGACAAA GCCTCAGAAG 
CATGTGCTGG ACAGCTGCTC TCCACTTCTT CGATATATCT CTCACGCTGA ATTCAAAGAC 
TTACTGTTGC CAACTCTGCA GAAATCCTTG CTACGGAGCC CTGAGAATGT AATTGAAACA 
ATCTCTTGCT TGCTCTTGTC TGTTAACCTG GATCTTAGCC AGTATGCTCT GGACATTGTG 
AAAGGACTGG CTGGTCATCT GAAATCCAAC AGTGTGCAGC TCATGGATGA AGCAGTTGTC 
GCATTAAAGA ATTTGGCTCG ACAGTGTAGT GATCCTTCAG CCGTGGAATC CCTGAGCAGA 
CACCTTTTTG CCATCTTAGG GGGCTCAGAG GGGAAGCTGA CAGTTGTTGC GCAGAAGATG 
AGCGTCCTTT CAGGGATTGG CAGCCTTAGT CACCATGTGG TTTCTGGATC ATCTAGCCAA 
GCTCTGGCTG GAACCGTGGC TGAGCTTTTC ATCCCTTTTC TGCAGCAAGA AGTCCACGAA 
GGCACGTTGG TTCATGCAGT CTCTGTCCTG GCTCTGTGGT GCGTTCGCTT CACCACTGAA 
GTCCCTAAGA AGATGGTGGA ATGGCTAAAG AAAGCATTTG GCCTTAAAAC CTCTACTTCA 
GCTGTGAGGC ATGCCTACCT GCAGTGCATG CTAGCCTCTT TCAAAGGTGA CACGTTATTA 
CAGGCAATGG ACTTGCTACC GATGCTGATC CAGACAGTAG AAAAAGCAGC ATCTCAAAGT 
ACTCAGATTC CCATGGTAAC AGAAGGAGTT GCTGCAGCTT TGTTGATCTG CAGGATGTCT 
GTAATTGAGG GACAAACTGA GTCTAAGCTG AGTGGCTTCT GGCAGCTGAT CCTAGATGAG 
AAAAAACAAA TTTTTACCTC TGAGAAGTTC TTGCAGTCTG CTTCAGAGGA AGCAATGTGC 
ACTGTGCTGC AGCTGACAGA GCGCCTCCTC TTGGACCATG CACAGCGGCT CCCCGAGAGT 
AAAATCCAGC AGTATCACCG TGCCCTTGTT GCAGTGCTGC TGAGTCGGAC CTGGCACGTT 
CGAAGACAGG CCCATCAGAC AGTTAGGAAG CTTTTGTCCT CTCTGGGAGG GTACAAACTG 
GCCTACGGGC TCTTAGAGGA GCTAAAAGTG GTTCTCAGCT CTCATAAGGT TATGCCTCAA 
GAAGTCCTGG TGATGGAGTC AGGTGAGCTG TCAGAACAAG GGAAGACTTA TATTCCTCCC 
CGTGTCCTTC ATGAGGCACT GTGTGTCATC TCAAGTGTAG CTGGGCTGGA TGTGGATTCC 
TTTGAGACAG AAAAGCTGGC TCTGGAGATG CTGCTGGTGA GCCATCATCC ATCTCTGGTG 
GCAATACAGC CAGGTCTTTG GCCAGCCCTC CTTGTCAAGA TGAAGATAGA TCCCAAAGAC 
TTTATTACTA AACACCTGAA TGACATATTA CCCAGACTCA CTGCCTACTC TCCAGACAAC 
CAGTCATCTA TGAATGCAGT GGGATCTCTC TCTCTGCTCT CACCTGGTAG AGTGCTCCCA 
CAGCTCATCA GCACCATCTC AGCATCAATG CAGAATCCAG CTCTGTGCCA GGTTACTCGA 
GAGGAATTTG CCATCATGAA GACACCGGAG GGAGAACTGT ATGATAAGTC TATAATACAG 
AGTGCTCAAC AGGATAGTAT GAAAAAGGCT AACATGAAGA GGGAGAATAA AGCCTACTCC 
TTCAAGGAAC AAATCATTGA GCTGGAGCTG AAGGAGGAGA TAAAGAAGAA GAAGGGGATA 
AAGGATGAGG TGCAGCTGAC TAGCAAGCAG AAAGAGCTGA TGCATGCACA GCTGGAAAAG 
GAATCTCAGA TAAGAAAACG ACTCAAGGAG CTTGATAATG AACTGGAAAC AGCCTTGGGA 
CTGCTGGACA CTGTTATCAA GAGAAAACCT CCTGGTCTAA CTCAGTACAT CCCTAGTCTT 
GTTGGTTCCT TTTTGCCACT TTTCAGATCG CCTTTGGCTG CTCCAAGAAT TAAGGATCCT 
TTTCTTTCCT TAGCTTCCTG TGTCATGCCT GCTCGACTTA AAACCTTTGG TACTTTAGTG 
AGCCACGTGA CCTTGCGCCT GATGAAACCA GAATGTGAGT TAGATGAATC GTGGTGTCAA 
GAAGAGCTTC CTACTGCTCT CAACAGAGTA GTAAGCTTGC TGCATGCCCA CACCATTCCT 
AGCCGAACTG GCAAAGGAGA GGCAGGTGCT GCCCCATTAT CAGCTCCAGC ATTTTCCCTA 
GTCTTTCCTC TCCTAAAGAC GGCTATGACT GAGACATCCC ATGACAGTGA AGATAAGGAG 
GAATTCCTGG TCAAGATTCT GCAGATCCTT ACAGTTCATG CTCAGCTGAG ATCATCCACA 
AACGGCCAGG CTTTGCTGGT GGATGAGAAT GGCCCGGAGT TGCTGCCCCG ACGGGACATG 
CTGCTTCTGC TGACCCGAGT GATAGCAACA GGCTCTCCAC GGTTACAGGT CTTGGCTTCC 
AATGCACTGA CCTCTGTGTG CACAAGTAGC AGTGGAGAGG ATGGGTGTGC CTATGCAGAA 
CAAGAGGAGA TTGATGTGTT ACTTCAGGCC CTGCAGTCTC CATGCATGAA TGTTCGAGAT 
GCAGCTCTCA GGGGACTGAT GGAGCTACAG ATGGTTCTAC CAACCCCAGA CAGCGATGAA 
AAGAATGGAT TAAATCTGTT GCGTCGCCTT TGGGTAGTAA AGTTTGATGT GGAAGATGAG 
ATTCAGAAGC TGGCAGAGAG GTTGTGGGAG TCCATGGGTC TGGAACTACA GCCTGACCTC 
TGTTCTCTGC TGATCAAGGA TGTCATCTAC CATGAAGAGG CAGTGCGACA AGCAGGTGCC 
GAAGCTCTTT CCAAAGCTGT AGCTCAGTAT CAGCACCAGG CAGCAGAGGT TATGAATAAA 
CTTACAGAGA TTTACCAGGA GAAGCTCTAT AGGCCACCTC CAGTTTTGGA TGCTTTGGGA 
CGAGTGATAT CTGAATCACC ACCTGACCAG TGGGAAGCAA GGTGTGGCAT TGCTTTGGCC 
CTTAACAAGC TCTCAGAGCA TCTGGACAGT TCTCAGGTGA AGCCACTCTT TCAGTTTTTT 
GTACCAGATG CTCTGAATGA TCGCAATCCT GAAGTCAGGA AGTGCATGTT AGATGCAGCT 
CTTTCAACGC TCAACACTCA TGGCAAAGAC AATGTTAACT CTCTCTTGCC AGTGTTCGAA 
GAGTTCCTGA AAAATGCTCC TAATGATGCC AGTTATGATG CTGTCAGGCA GAGTGTGGTC 
ATTCTGATGG GCTCACTGGC AAAACATCTG GACAAAAGTG ACCCTAAAGT GAAACCAATT 
GTTGGCAAAC TGATTGCAGC CTTGTCCACA CCATCTCAGC AGGTTCAAGA GTCAGTGGCA 
AGCTGTCTTC CACCACTGGT GCCTGCAATT AAGGAGGATG CAGGAGGAAT GATACAGAAG 
CTAATGCAGC TGCTTTTAGA ATCTGATAAA TATGCCGAGC GCAAAGGTGC AGCTTATGGA 
CTGGCAGGTC TGGTGAAAGG CCTTGGCATC CTTTCTCTCA AGCAGCAGGA GATGATGACC 
ACACTGACTG ATGCTATCCA AGACAAGAAG AACTTTCGTC GGCGGGAGGG GGCTCTATTT 
GCCTTTGAGA TGCTCTGCAG CATGCTTGGA AAGCTCTTTG AGCCCTATGT GGTTCATGTG 
CTACCTCACT TGTTGCTTTG TTTTGGAGAT GGGAACCAGT ATGTGCGAGA GGCTGCAGAT 
GACTGTGCCA AAGCAGTGAT GAGCAACTTG AGTACTCATG GAGTGAAGCT GGTTTTGCCA 
TCACTTCTTG CTGCACTAGA AGAGGAATCT TGGAGAACCA AAGCTGGGTC AGTGGAACTG 
CTGGGAGCTA TGGCATACTG TGCTCCCAAA CAGCTCTCCT CCTGCTTACC CAATATTGTG 
CCAAAACTTA CTGAAGTGCT CACAGATTCT CATGTGAAGG TGCAGAATGC AGGTCAGCAA 
GCTCTCAGGC AGATTGGGTC CGTCATCAGA AATCCAGAGA TCCTTGCAAT TGCTCCAGTT 
TTGTTGGATG CTCTCACTGA CCCCTCCAGG AAGACCCAAA AGTGTCTGCA GACTCTACTG 
GATACCAAAT TTGTCCACTT CATTGATGCT CCATCCTTAG CACTTATTAT GCCAATTGTT 
CAGAGAGCTT TTCAGGATCG TTCCACAGAT ACCAGGAAAA TGGCTGCCCA GATTATTGGG 
AACATGTACT CCTTGACTGA TCAGAAGGAT CTGGCTCCTT ACCTACCCAG TGTGACTCCT 
GGACTGAAGG CATCCCTGCT GGATCCTGTA CCTGAAGTGC GTACAGTATC TGCAAAGGCA 
CTGGGAGCCA TGGTGAAGGG CATGGGAGAA TCATGCTTTG AGGATTTGTT GCCATGGCTG 
ATGGAGACAC TGACATACGA GCAAAGCTCA GTGGACCGAT CTGGTGCAGC TCAAGGTCTG 
GCTGAAGTTA TGGCTGGTTT AGGGGTGGAG AAGCTGGAGA AACTTATGCC AGAGATTGTA 
GCTACTGCCA GTAAGGTGGA TATTGCTCCA CATGTACGAG ATGGTTATAT AATGATGTTT 
AACTACCTGC CAATTACTTT TGGAGACAAG TTCATTCCGT ATGTTGGTCC CATCATCCCT 
TGTATCCTTA AGGCACTGGC AGATGAGAAT GAGTTTGTGC GGGACACTGC CCTGCGTGCT 
GGCCAGAGGA TCATAAGTAT GTATGCAGAG ACAGCCATTG CACTGCTTCT GCCACAGCTT 
GAGGATGGCC TGTTTGATGA CCTGTGGAGG ATAAGGTTTA GCTCAGTTCA GCTCCTTGGA 
GATCTTTTAT TCCATATCTC AGGAGTCACT GGAAAAATGA CCACAGAAAC TGCCTCAGAA 
GATGACAACT TTGGAACAGC CCAGTCAAAC AAGGCTATTA TTAATGCTCT TGGGGTGGAG 
AGGAGGAACA GGGTGCTGGC AGGGCTGTAC ATGGGCCGCT CTGACACCCA GCTGGTAGTG 
CGTCAAGCTT CGTTACACGT TTGGAAGATT GTAGTTTCAA ACACTCCTCG CACGCTGCGT 
GAAATTTTGC CAACACTCTT TGGGCTTCTG CTGAAATTCT TGGCCAGTAC TTGTGCAGAT 
AAGCGAACGG TTGCAGCACG GACATTAGGA GACCTTGTCC GAAAGTTAGG AGAGAAGATC 
CTTCCTGAAA TCATTCCCAT TCTGGAAGAT GGCCTAAGGT CAGATAAGAA TGATGAAAGG 
CAAGGAGTCT GCATTGGGCT GAGTGAGATA ATGAAGTCTA CCAGTAGAGA TGCGGTACTG 
GTTTTCTCTG AGTCCCTAGT GCCTACAGTG AGAAAAGCAT TGTGTGACCC TCTGGAAGAA 
GTGAGAGAGG CTGCAGCTAA AACATTTGAA CAGTTGCACT CAACAATTGG GCATCAGGCT 
CTTGAAGATA TCCTTCCGTT TTTGTTGAAG CAGCTGGATA ATGAAGAAAC ATCTGACTTT 
GCTGTGGATG GTCTTAAACA AGTTATGGCT GTCAAGAGCC GTGTGGTACT GCCTTATTTG 
GTGCCAAAGC TGACTACTCC CCCTGTGAAC ACCCGTGTGC TAGCTTTCCT CTCATCAGTG 
GCAGGGGATG CTCTGACAAG GCACTTGAGT GTCATCCTGC CTGCTATGAT GTCTGCACTG 
AAAGAGAAGC TGGGGACTAG TGAAGAGCAA CTGGAAATGG CAAATTGCCA GTCTGTAATC 
CTTTCTGTGG AAGATGATGT TGGACAAAGA ATTATAACTG AGGATTTACT AGAAGCTACC 
CGCAGCCCTG ACGTGGGAAT GAGACAAGCA GCAGCTATCA TTCTGAATAT CTACTGCTCC 
AAGACAAAAG CAGACTACAC TGGTCATCTC AAGAACTTGG TTTCAGGCCT GATACGTTTA 
TTTAATGATA CCAATCCTGT AGTTTTGAAT GAAAGCTGGG ATGCACTTAA TTCCATCACC 
AAGAAATTAG ATGCTGGGAA CCAGCTTGCT CTCATTGAGG ACCTACACAA GGACATCCGG 
GTTGTAGGGA ATGAGGCCAA AGGCGAGCAT GTGCCAGGAT TTTGTATTCC TAAGAAGGGA 
GTGACTTCTA TACTTCTGGT GCTGCGTGAA GGTGTTCTAA CTGGTAATCC AGAGCAAAAG 
GAGGAAGCAG CAAAGGCCTT GGGGCTGGTT ATTAAGCTGA CTTCTGCGGA AGCTCTTAAA 
CCATCTGTAG TGAGCATTAC TGGGCCACTT ATTCGTATCT TGGGAGATCG GTTCAGCTGG 
AATGTCAAAG TGGCTCTGCT TGAAACACTA AGCCTTCTTT TAGCCAAGGT TGAGATTGCC 
CTGAAACCAT TTTTGCCTCA ACTGCAAACA ACCTTCACCA AAGCTCTGCA AGATTCAAAC 
CGTGCTGTTC GCCTTAAAGC TGCTGATGCT CTTGGTAAAC TTATTGTTAT CCATGTAAAA 
GTGGATCCTC TTTTTACTGA GCTGCTGAAT GGAATTCGTT CCAGTGATGA CTCTGCCATC 
AGGGACACGA TGCTGCAAGC ACTGCGGTTT GTAACACGAG GAGCTGGTGC AAAAGTAGAT 
GCCACAATTA GGAAGAACAT CAGCACAGTT CTTCTTGGGA TGCTGGGACA CGATGAGGAT 
GCCACCCGCA TGGCTTCAGC TGGCTGCTTA GCAGAACTGT GTGCATTTCT GTCAGAAGAG 
GAGCTCAATA CTGTTCTCCA CCAGCACTTA CTGGCAGACA TCTCTGGAAT TGACTGGATG 
GTGAGACATG GACGAAGCCT GGCCCTCTCA GTGGCTGTGA ATGTTGCTCC TTATAGGCTG 
TGTTCCCCCA AGTACTATAA CAGTGTTCTG GAGATGATCC TCAGCAATGC CACTGCTGAC 
AGGATACCCA TTGCAGTTAG TGGCATCAGA GGGATGGGCT TTCTTATGAA ATACCACATG 
GAAGAAGGAG GGAACCTGCC TCCAAAGCTT TCCAACCTCT TTATTAAGTG TCTCCAGAAC 
TCATCCAGTG ACATAAAGTT AGTTGCAGAA AAAATGATCT GGTGGGCAAA TAAAAACCAT 
CTTCCTGCAC TGGATCCTCA GACAATTAAA CCAATTCTCA AAGCGCTTCT GGACAACACA 
AAAGACAAAA ACACCAGTGT AAGAGCTTAC AGCGACCAAG CCATTGTTAA TCTTCTGAAG 
ATGAGAGTGG GTGAACAAGT GCTTGAGTCT GTTTCCAAGA TTTTAGATGC TGCCAGCTTG 
GAGCTTCTCA ATGAAAGCTG CAGGAGATCT CTTAAGAAGC TGGCTGGCCA GGCTGACTCT 
GATGAACAGA TAGATGACAC TATACTGACG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCN1 ( XM_015275397.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015130883.1 Gallus gallus GCN1, eIF2 alpha kinase activator homolog (GCN1), transcript variant X1, mRNA.

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CloneID	OGa42822
Clone ID Related Accession (Same CDS sequence)	XM_015275397.1 , XM_015275397.2
Accession Version	XM_015275397.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	eIF-2-alpha kinase activator GCN1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006102.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015275397.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG ACACGCAGAT ATCTGACACA CTGAAGCGGT TTGCAGTGAA GGTAACTACA 
GCCAGCGTGA AGGAGCGGAG GGAGATCCTC AGTGAGCTGG GAAAATGCAT TTCTGGGAAA 
GATCTTCCCG AGGGTGCAGT GAAAGGCCTT TGCAAACTCT TCTGCCTTAC TCTGCACCGA 
TACCGCGACG CGGCGTCGCG CAGAGCCCTG CAGTTGGCGC TTCAGCAGCT CGCGGAGTCC 
CAGCCAGATG CAACAGCAAA AAACCTTCTG CAGTCACTTC AGTCTTCGGG GATCAGTGGC 
AAGGCTGGAG CTCCCAGTAA GAGCAGTGGA TCTGCAGCCC TGCTGGCACT CTCCTGGACA 
TGCCTGCTGG TACGGACAGT CTTTCCATCA CCTGACAAGA GACAGGGAGA AACCTGGAAA 
AAACTGGTGG AGGTTCAATG TTTACTTCTT TTGGAAGTCT TGGGAGGCTC TCATAAGCAG 
GCAGTGGTTG GAGCAGTGAA GAAACTGAAC CGACTTTGGA AAGAGAACCA AGATCTGGTG 
GATCAGTACC TATCTGTCAT TCTTGGTCTT GAACCAAACC AGAGCTATGC TGCAATGCTG 
GGACTTGTGG TGCAGTTTTG CACAGCTCAG AAAGAGACTG ATATTATTAA ACGCCACAAG 
AGTGCTCTTC TGGATTTCTA TGTGAAGACC ATCCTGATGA GCAAGACAAA GCCTCAGAAG 
CATGTGCTGG ACAGCTGCTC TCCACTTCTT CGATATATCT CTCACGCTGA ATTCAAAGAC 
TTACTGTTGC CAACTCTGCA GAAATCCTTG CTACGGAGCC CTGAGAATGT AATTGAAACA 
ATCTCTTGCT TGCTCTTGTC TGTTAACCTG GATCTTAGCC AGTATGCTCT GGACATTGTG 
AAAGGACTGG CTGGTCATCT GAAATCCAAC AGTGTGCAGC TCATGGATGA AGCAGTTGTC 
GCATTAAAGA ATTTGGCTCG ACAGTGTAGT GATCCTTCAG CCGTGGAATC CCTGAGCAGA 
CACCTTTTTG CCATCTTAGG GGGCTCAGAG GGGAAGCTGA CAGTTGTTGC GCAGAAGATG 
AGCGTCCTTT CAGGGATTGG CAGCCTTAGT CACCATGTGG TTTCTGGATC ATCTAGCCAA 
GCTCTGGCTG GAACCGTGGC TGAGCTTTTC ATCCCTTTTC TGCAGCAAGA AGTCCACGAA 
GGCACGTTGG TTCATGCAGT CTCTGTCCTG GCTCTGTGGT GCGTTCGCTT CACCACTGAA 
GTCCCTAAGA AGATGGTGGA ATGGCTAAAG AAAGCATTTG GCCTTAAAAC CTCTACTTCA 
GCTGTGAGGC ATGCCTACCT GCAGTGCATG CTAGCCTCTT TCAAAGGTGA CACGTTATTA 
CAGGCAATGG ACTTGCTACC GATGCTGATC CAGACAGTAG AAAAAGCAGC ATCTCAAAGT 
ACTCAGATTC CCATGGTAAC AGAAGGAGTT GCTGCAGCTT TGTTGATCTG CAGGATGTCT 
GTAATTGAGG GACAAACTGA GTCTAAGCTG AGTGGCTTCT GGCAGCTGAT CCTAGATGAG 
AAAAAACAAA TTTTTACCTC TGAGAAGTTC TTGCAGTCTG CTTCAGAGGA AGCAATGTGC 
ACTGTGCTGC AGCTGACAGA GCGCCTCCTC TTGGACCATG CACAGCGGCT CCCCGAGAGT 
AAAATCCAGC AGTATCACCG TGCCCTTGTT GCAGTGCTGC TGAGTCGGAC CTGGCACGTT 
CGAAGACAGG CCCATCAGAC AGTTAGGAAG CTTTTGTCCT CTCTGGGAGG GTACAAACTG 
GCCTACGGGC TCTTAGAGGA GCTAAAAGTG GTTCTCAGCT CTCATAAGGT TATGCCTCAA 
GAAGTCCTGG TGATGGAGTC AGGTGAGCTG TCAGAACAAG GGAAGACTTA TATTCCTCCC 
CGTGTCCTTC ATGAGGCACT GTGTGTCATC TCAAGTGTAG CTGGGCTGGA TGTGGATTCC 
TTTGAGACAG AAAAGCTGGC TCTGGAGATG CTGCTGGTGA GCCATCATCC ATCTCTGGTG 
GCAATACAGC CAGGTCTTTG GCCAGCCCTC CTTGTCAAGA TGAAGATAGA TCCCAAAGAC 
TTTATTACTA AACACCTGAA TGACATATTA CCCAGACTCA CTGCCTACTC TCCAGACAAC 
CAGTCATCTA TGAATGCAGT GGGATCTCTC TCTCTGCTCT CACCTGGTAG AGTGCTCCCA 
CAGCTCATCA GCACCATCTC AGCATCAATG CAGAATCCAG CTCTGTGCCA GGTTACTCGA 
GAGGAATTTG CCATCATGAA GACACCGGAG GGAGAACTGT ATGATAAGTC TATAATACAG 
AGTGCTCAAC AGGATAGTAT GAAAAAGGCT AACATGAAGA GGGAGAATAA AGCCTACTCC 
TTCAAGGAAC AAATCATTGA GCTGGAGCTG AAGGAGGAGA TAAAGAAGAA GAAGGGGATA 
AAGGATGAGG TGCAGCTGAC TAGCAAGCAG AAAGAGCTGA TGCATGCACA GCTGGAAAAG 
GAATCTCAGA TAAGAAAACG ACTCAAGGAG CTTGATAATG AACTGGAAAC AGCCTTGGGA 
CTGCTGGACA CTGTTATCAA GAGAAAACCT CCTGGTCTAA CTCAGTACAT CCCTAGTCTT 
GTTGGTTCCT TTTTGCCACT TTTCAGATCG CCTTTGGCTG CTCCAAGAAT TAAGGATCCT 
TTTCTTTCCT TAGCTTCCTG TGTCATGCCT GCTCGACTTA AAACCTTTGG TACTTTAGTG 
AGCCACGTGA CCTTGCGCCT GATGAAACCA GAATGTGAGT TAGATGAATC GTGGTGTCAA 
GAAGAGCTTC CTACTGCTCT CAACAGAGTA GTAAGCTTGC TGCATGCCCA CACCATTCCT 
AGCCGAACTG GCAAAGGAGA GGCAGGTGCT GCCCCATTAT CAGCTCCAGC ATTTTCCCTA 
GTCTTTCCTC TCCTAAAGAC GGCTATGACT GAGACATCCC ATGACAGTGA AGATAAGGAG 
GAATTCCTGG TCAAGATTCT GCAGATCCTT ACAGTTCATG CTCAGCTGAG ATCATCCACA 
AACGGCCAGG CTTTGCTGGT GGATGAGAAT GGCCCGGAGT TGCTGCCCCG ACGGGACATG 
CTGCTTCTGC TGACCCGAGT GATAGCAACA GGCTCTCCAC GGTTACAGGT CTTGGCTTCC 
AATGCACTGA CCTCTGTGTG CACAAGTAGC AGTGGAGAGG ATGGGTGTGC CTATGCAGAA 
CAAGAGGAGA TTGATGTGTT ACTTCAGGCC CTGCAGTCTC CATGCATGAA TGTTCGAGAT 
GCAGCTCTCA GGGGACTGAT GGAGCTACAG ATGGTTCTAC CAACCCCAGA CAGCGATGAA 
AAGAATGGAT TAAATCTGTT GCGTCGCCTT TGGGTAGTAA AGTTTGATGT GGAAGATGAG 
ATTCAGAAGC TGGCAGAGAG GTTGTGGGAG TCCATGGGTC TGGAACTACA GCCTGACCTC 
TGTTCTCTGC TGATCAAGGA TGTCATCTAC CATGAAGAGG CAGTGCGACA AGCAGGTGCC 
GAAGCTCTTT CCAAAGCTGT AGCTCAGTAT CAGCACCAGG CAGCAGAGGT TATGAATAAA 
CTTACAGAGA TTTACCAGGA GAAGCTCTAT AGGCCACCTC CAGTTTTGGA TGCTTTGGGA 
CGAGTGATAT CTGAATCACC ACCTGACCAG TGGGAAGCAA GGTGTGGCAT TGCTTTGGCC 
CTTAACAAGC TCTCAGAGCA TCTGGACAGT TCTCAGGTGA AGCCACTCTT TCAGTTTTTT 
GTACCAGATG CTCTGAATGA TCGCAATCCT GAAGTCAGGA AGTGCATGTT AGATGCAGCT 
CTTTCAACGC TCAACACTCA TGGCAAAGAC AATGTTAACT CTCTCTTGCC AGTGTTCGAA 
GAGTTCCTGA AAAATGCTCC TAATGATGCC AGTTATGATG CTGTCAGGCA GAGTGTGGTC 
ATTCTGATGG GCTCACTGGC AAAACATCTG GACAAAAGTG ACCCTAAAGT GAAACCAATT 
GTTGGCAAAC TGATTGCAGC CTTGTCCACA CCATCTCAGC AGGTTCAAGA GTCAGTGGCA 
AGCTGTCTTC CACCACTGGT GCCTGCAATT AAGGAGGATG CAGGAGGAAT GATACAGAAG 
CTAATGCAGC TGCTTTTAGA ATCTGATAAA TATGCCGAGC GCAAAGGTGC AGCTTATGGA 
CTGGCAGGTC TGGTGAAAGG CCTTGGCATC CTTTCTCTCA AGCAGCAGGA GATGATGACC 
ACACTGACTG ATGCTATCCA AGACAAGAAG AACTTTCGTC GGCGGGAGGG GGCTCTATTT 
GCCTTTGAGA TGCTCTGCAG CATGCTTGGA AAGCTCTTTG AGCCCTATGT GGTTCATGTG 
CTACCTCACT TGTTGCTTTG TTTTGGAGAT GGGAACCAGT ATGTGCGAGA GGCTGCAGAT 
GACTGTGCCA AAGCAGTGAT GAGCAACTTG AGTACTCATG GAGTGAAGCT GGTTTTGCCA 
TCACTTCTTG CTGCACTAGA AGAGGAATCT TGGAGAACCA AAGCTGGGTC AGTGGAACTG 
CTGGGAGCTA TGGCATACTG TGCTCCCAAA CAGCTCTCCT CCTGCTTACC CAATATTGTG 
CCAAAACTTA CTGAAGTGCT CACAGATTCT CATGTGAAGG TGCAGAATGC AGGTCAGCAA 
GCTCTCAGGC AGATTGGGTC CGTCATCAGA AATCCAGAGA TCCTTGCAAT TGCTCCAGTT 
TTGTTGGATG CTCTCACTGA CCCCTCCAGG AAGACCCAAA AGTGTCTGCA GACTCTACTG 
GATACCAAAT TTGTCCACTT CATTGATGCT CCATCCTTAG CACTTATTAT GCCAATTGTT 
CAGAGAGCTT TTCAGGATCG TTCCACAGAT ACCAGGAAAA TGGCTGCCCA GATTATTGGG 
AACATGTACT CCTTGACTGA TCAGAAGGAT CTGGCTCCTT ACCTACCCAG TGTGACTCCT 
GGACTGAAGG CATCCCTGCT GGATCCTGTA CCTGAAGTGC GTACAGTATC TGCAAAGGCA 
CTGGGAGCCA TGGTGAAGGG CATGGGAGAA TCATGCTTTG AGGATTTGTT GCCATGGCTG 
ATGGAGACAC TGACATACGA GCAAAGCTCA GTGGACCGAT CTGGTGCAGC TCAAGGTCTG 
GCTGAAGTTA TGGCTGGTTT AGGGGTGGAG AAGCTGGAGA AACTTATGCC AGAGATTGTA 
GCTACTGCCA GTAAGGTGGA TATTGCTCCA CATGTACGAG ATGGTTATAT AATGATGTTT 
AACTACCTGC CAATTACTTT TGGAGACAAG TTCATTCCGT ATGTTGGTCC CATCATCCCT 
TGTATCCTTA AGGCACTGGC AGATGAGAAT GAGTTTGTGC GGGACACTGC CCTGCGTGCT 
GGCCAGAGGA TCATAAGTAT GTATGCAGAG ACAGCCATTG CACTGCTTCT GCCACAGCTT 
GAGGATGGCC TGTTTGATGA CCTGTGGAGG ATAAGGTTTA GCTCAGTTCA GCTCCTTGGA 
GATCTTTTAT TCCATATCTC AGGAGTCACT GGAAAAATGA CCACAGAAAC TGCCTCAGAA 
GATGACAACT TTGGAACAGC CCAGTCAAAC AAGGCTATTA TTAATGCTCT TGGGGTGGAG 
AGGAGGAACA GGGTGCTGGC AGGGCTGTAC ATGGGCCGCT CTGACACCCA GCTGGTAGTG 
CGTCAAGCTT CGTTACACGT TTGGAAGATT GTAGTTTCAA ACACTCCTCG CACGCTGCGT 
GAAATTTTGC CAACACTCTT TGGGCTTCTG CTGAAATTCT TGGCCAGTAC TTGTGCAGAT 
AAGCGAACGG TTGCAGCACG GACATTAGGA GACCTTGTCC GAAAGTTAGG AGAGAAGATC 
CTTCCTGAAA TCATTCCCAT TCTGGAAGAT GGCCTAAGGT CAGATAAGAA TGATGAAAGG 
CAAGGAGTCT GCATTGGGCT GAGTGAGATA ATGAAGTCTA CCAGTAGAGA TGCGGTACTG 
GTTTTCTCTG AGTCCCTAGT GCCTACAGTG AGAAAAGCAT TGTGTGACCC TCTGGAAGAA 
GTGAGAGAGG CTGCAGCTAA AACATTTGAA CAGTTGCACT CAACAATTGG GCATCAGGCT 
CTTGAAGATA TCCTTCCGTT TTTGTTGAAG CAGCTGGATA ATGAAGAAAC ATCTGACTTT 
GCTGTGGATG GTCTTAAACA AGTTATGGCT GTCAAGAGCC GTGTGGTACT GCCTTATTTG 
GTGCCAAAGC TGACTACTCC CCCTGTGAAC ACCCGTGTGC TAGCTTTCCT CTCATCAGTG 
GCAGGGGATG CTCTGACAAG GCACTTGAGT GTCATCCTGC CTGCTATGAT GTCTGCACTG 
AAAGAGAAGC TGGGGACTAG TGAAGAGCAA CTGGAAATGG CAAATTGCCA GTCTGTAATC 
CTTTCTGTGG AAGATGATGT TGGACAAAGA ATTATAACTG AGGATTTACT AGAAGCTACC 
CGCAGCCCTG ACGTGGGAAT GAGACAAGCA GCAGCTATCA TTCTGAATAT CTACTGCTCC 
AAGACAAAAG CAGACTACAC TGGTCATCTC AAGAACTTGG TTTCAGGCCT GATACGTTTA 
TTTAATGATA CCAATCCTGT AGTTTTGAAT GAAAGCTGGG ATGCACTTAA TTCCATCACC 
AAGAAATTAG ATGCTGGGAA CCAGCTTGCT CTCATTGAGG ACCTACACAA GGACATCCGG 
GTTGTAGGGA ATGAGGCCAA AGGCGAGCAT GTGCCAGGAT TTTGTATTCC TAAGAAGGGA 
GTGACTTCTA TACTTCTGGT GCTGCGTGAA GGTGTTCTAA CTGGTAATCC AGAGCAAAAG 
GAGGAAGCAG CAAAGGCCTT GGGGCTGGTT ATTAAGCTGA CTTCTGCGGA AGCTCTTAAA 
CCATCTGTAG TGAGCATTAC TGGGCCACTT ATTCGTATCT TGGGAGATCG GTTCAGCTGG 
AATGTCAAAG TGGCTCTGCT TGAAACACTA AGCCTTCTTT TAGCCAAGGT TGAGATTGCC 
CTGAAACCAT TTTTGCCTCA ACTGCAAACA ACCTTCACCA AAGCTCTGCA AGATTCAAAC 
CGTGCTGTTC GCCTTAAAGC TGCTGATGCT CTTGGTAAAC TTATTGTTAT CCATGTAAAA 
GTGGATCCTC TTTTTACTGA GCTGCTGAAT GGAATTCGTT CCAGTGATGA CTCTGCCATC 
AGGGACACGA TGCTGCAAGC ACTGCGGTTT GTAACACGAG GAGCTGGTGC AAAAGTAGAT 
GCCACAATTA GGAAGAACAT CAGCACAGTT CTTCTTGGGA TGCTGGGACA CGATGAGGAT 
GCCACCCGCA TGGCTTCAGC TGGCTGCTTA GCAGAACTGT GTGCATTTCT GTCAGAAGAG 
GAGCTCAATA CTGTTCTCCA CCAGCACTTA CTGGCAGACA TCTCTGGAAT TGACTGGATG 
GTGAGACATG GACGAAGCCT GGCCCTCTCA GTGGCTGTGA ATGTTGCTCC TTATAGGCTG 
TGTTCCCCCA AGTACTATAA CAGTGTTCTG GAGATGATCC TCAGCAATGC CACTGCTGAC 
AGGATACCCA TTGCAGTTAG TGGCATCAGA GGGATGGGCT TTCTTATGAA ATACCACATG 
GAAGAAGGAG GGAACCTGCC TCCAAAGCTT TCCAACCTCT TTATTAAGTG TCTCCAGAAC 
TCATCCAGTG ACATAAAGTT AGTTGCAGAA AAAATGATCT GGTGGGCAAA TAAAAACCAT 
CTTCCTGCAC TGGATCCTCA GACAATTAAA CCAATTCTCA AAGCGCTTCT GGACAACACA 
AAAGACAAAA ACACCAGTGT AAGAGCTTAC AGCGACCAAG CCATTGTTAA TCTTCTGAAG 
ATGAGAGTGG GTGAACAAGT GCTTGAGTCT GTTTCCAAGA TTTTAGATGC TGCCAGCTTG 
GAGCTTCTCA ATGAAAGCTG CAGGAGATCT CTTAAGAAGC TGGCTGGCCA GGCTGACTCT 
GATGAACAGA TAGATGACAC TATACTGACG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015130883.1
CDS	1..8013
Translation	MAADTQISDTLKRFAVKVTTASVKERREILSELGKCISGKDLPEGAVKGLCKLFCLTLHRYRDAASRRALQLALQQLAESQPDATAKNLLQSLQSSGISGKAGAPSKSSGSAALLALSWTCLLVRTVFPSPDKRQGETWKKLVEVQCLLLLEVLGGSHKQAVVGAVKKLNRLWKENQDLVDQYLSVILGLEPNQSYAAMLGLVVQFCTAQKETDIIKRHKSALLDFYVKTILMSKTKPQKHVLDSCSPLLRYISHAEFKDLLLPTLQKSLLRSPENVIETISCLLLSVNLDLSQYALDIVKGLAGHLKSNSVQLMDEAVVALKNLARQCSDPSAVESLSRHLFAILGGSEGKLTVVAQKMSVLSGIGSLSHHVVSGSSSQALAGTVAELFIPFLQQEVHEGTLVHAVSVLALWCVRFTTEVPKKMVEWLKKAFGLKTSTSAVRHAYLQCMLASFKGDTLLQAMDLLPMLIQTVEKAASQSTQIPMVTEGVAAALLICRMSVIEGQTESKLSGFWQLILDEKKQIFTSEKFLQSASEEAMCTVLQLTERLLLDHAQRLPESKIQQYHRALVAVLLSRTWHVRRQAHQTVRKLLSSLGGYKLAYGLLEELKVVLSSHKVMPQEVLVMESGELSEQGKTYIPPRVLHEALCVISSVAGLDVDSFETEKLALEMLLVSHHPSLVAIQPGLWPALLVKMKIDPKDFITKHLNDILPRLTAYSPDNQSSMNAVGSLSLLSPGRVLPQLISTISASMQNPALCQVTREEFAIMKTPEGELYDKSIIQSAQQDSMKKANMKRENKAYSFKEQIIELELKEEIKKKKGIKDEVQLTSKQKELMHAQLEKESQIRKRLKELDNELETALGLLDTVIKRKPPGLTQYIPSLVGSFLPLFRSPLAAPRIKDPFLSLASCVMPARLKTFGTLVSHVTLRLMKPECELDESWCQEELPTALNRVVSLLHAHTIPSRTGKGEAGAAPLSAPAFSLVFPLLKTAMTETSHDSEDKEEFLVKILQILTVHAQLRSSTNGQALLVDENGPELLPRRDMLLLLTRVIATGSPRLQVLASNALTSVCTSSSGEDGCAYAEQEEIDVLLQALQSPCMNVRDAALRGLMELQMVLPTPDSDEKNGLNLLRRLWVVKFDVEDEIQKLAERLWESMGLELQPDLCSLLIKDVIYHEEAVRQAGAEALSKAVAQYQHQAAEVMNKLTEIYQEKLYRPPPVLDALGRVISESPPDQWEARCGIALALNKLSEHLDSSQVKPLFQFFVPDALNDRNPEVRKCMLDAALSTLNTHGKDNVNSLLPVFEEFLKNAPNDASYDAVRQSVVILMGSLAKHLDKSDPKVKPIVGKLIAALSTPSQQVQESVASCLPPLVPAIKEDAGGMIQKLMQLLLESDKYAERKGAAYGLAGLVKGLGILSLKQQEMMTTLTDAIQDKKNFRRREGALFAFEMLCSMLGKLFEPYVVHVLPHLLLCFGDGNQYVREAADDCAKAVMSNLSTHGVKLVLPSLLAALEEESWRTKAGSVELLGAMAYCAPKQLSSCLPNIVPKLTEVLTDSHVKVQNAGQQALRQIGSVIRNPEILAIAPVLLDALTDPSRKTQKCLQTLLDTKFVHFIDAPSLALIMPIVQRAFQDRSTDTRKMAAQIIGNMYSLTDQKDLAPYLPSVTPGLKASLLDPVPEVRTVSAKALGAMVKGMGESCFEDLLPWLMETLTYEQSSVDRSGAAQGLAEVMAGLGVEKLEKLMPEIVATASKVDIAPHVRDGYIMMFNYLPITFGDKFIPYVGPIIPCILKALADENEFVRDTALRAGQRIISMYAETAIALLLPQLEDGLFDDLWRIRFSSVQLLGDLLFHISGVTGKMTTETASEDDNFGTAQSNKAIINALGVERRNRVLAGLYMGRSDTQLVVRQASLHVWKIVVSNTPRTLREILPTLFGLLLKFLASTCADKRTVAARTLGDLVRKLGEKILPEIIPILEDGLRSDKNDERQGVCIGLSEIMKSTSRDAVLVFSESLVPTVRKALCDPLEEVREAAAKTFEQLHSTIGHQALEDILPFLLKQLDNEETSDFAVDGLKQVMAVKSRVVLPYLVPKLTTPPVNTRVLAFLSSVAGDALTRHLSVILPAMMSALKEKLGTSEEQLEMANCQSVILSVEDDVGQRIITEDLLEATRSPDVGMRQAAAIILNIYCSKTKADYTGHLKNLVSGLIRLFNDTNPVVLNESWDALNSITKKLDAGNQLALIEDLHKDIRVVGNEAKGEHVPGFCIPKKGVTSILLVLREGVLTGNPEQKEEAAKALGLVIKLTSAEALKPSVVSITGPLIRILGDRFSWNVKVALLETLSLLLAKVEIALKPFLPQLQTTFTKALQDSNRAVRLKAADALGKLIVIHVKVDPLFTELLNGIRSSDDSAIRDTMLQALRFVTRGAGAKVDATIRKNISTVLLGMLGHDEDATRMASAGCLAELCAFLSEEELNTVLHQHLLADISGIDWMVRHGRSLALSVAVNVAPYRLCSPKYYNSVLEMILSNATADRIPIAVSGIRGMGFLMKYHMEEGGNLPPKLSNLFIKCLQNSSSDIKLVAEKMIWWANKNHLPALDPQTIKPILKALLDNTKDKNTSVRAYSDQAIVNLLKMRVGEQVLESVSKILDAASLELLNESCRRSLKKLAGQADSDEQIDDTILT

Target ORF information:

RefSeq Version	XM_015275397.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus GCN1, eIF2 alpha kinase activator homolog (GCN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015275397.2

ORF Insert Sequence:

ATGGCGGCCG ACACGCAGAT ATCTGACACA CTGAAGCGGT TTGCAGTGAA GGTAACTACA 
GCCAGCGTGA AGGAGCGGAG GGAGATCCTC AGTGAGCTGG GAAAATGCAT TTCTGGGAAA 
GATCTTCCCG AGGGTGCAGT GAAAGGCCTT TGCAAACTCT TCTGCCTTAC TCTGCACCGA 
TACCGCGACG CGGCGTCGCG CAGAGCCCTG CAGTTGGCGC TTCAGCAGCT CGCGGAGTCC 
CAGCCAGATG CAACAGCAAA AAACCTTCTG CAGTCACTTC AGTCTTCGGG GATCAGTGGC 
AAGGCTGGAG CTCCCAGTAA GAGCAGTGGA TCTGCAGCCC TGCTGGCACT CTCCTGGACA 
TGCCTGCTGG TACGGACAGT CTTTCCATCA CCTGACAAGA GACAGGGAGA AACCTGGAAA 
AAACTGGTGG AGGTTCAATG TTTACTTCTT TTGGAAGTCT TGGGAGGCTC TCATAAGCAG 
GCAGTGGTTG GAGCAGTGAA GAAACTGAAC CGACTTTGGA AAGAGAACCA AGATCTGGTG 
GATCAGTACC TATCTGTCAT TCTTGGTCTT GAACCAAACC AGAGCTATGC TGCAATGCTG 
GGACTTGTGG TGCAGTTTTG CACAGCTCAG AAAGAGACTG ATATTATTAA ACGCCACAAG 
AGTGCTCTTC TGGATTTCTA TGTGAAGACC ATCCTGATGA GCAAGACAAA GCCTCAGAAG 
CATGTGCTGG ACAGCTGCTC TCCACTTCTT CGATATATCT CTCACGCTGA ATTCAAAGAC 
TTACTGTTGC CAACTCTGCA GAAATCCTTG CTACGGAGCC CTGAGAATGT AATTGAAACA 
ATCTCTTGCT TGCTCTTGTC TGTTAACCTG GATCTTAGCC AGTATGCTCT GGACATTGTG 
AAAGGACTGG CTGGTCATCT GAAATCCAAC AGTGTGCAGC TCATGGATGA AGCAGTTGTC 
GCATTAAAGA ATTTGGCTCG ACAGTGTAGT GATCCTTCAG CCGTGGAATC CCTGAGCAGA 
CACCTTTTTG CCATCTTAGG GGGCTCAGAG GGGAAGCTGA CAGTTGTTGC GCAGAAGATG 
AGCGTCCTTT CAGGGATTGG CAGCCTTAGT CACCATGTGG TTTCTGGATC ATCTAGCCAA 
GCTCTGGCTG GAACCGTGGC TGAGCTTTTC ATCCCTTTTC TGCAGCAAGA AGTCCACGAA 
GGCACGTTGG TTCATGCAGT CTCTGTCCTG GCTCTGTGGT GCGTTCGCTT CACCACTGAA 
GTCCCTAAGA AGATGGTGGA ATGGCTAAAG AAAGCATTTG GCCTTAAAAC CTCTACTTCA 
GCTGTGAGGC ATGCCTACCT GCAGTGCATG CTAGCCTCTT TCAAAGGTGA CACGTTATTA 
CAGGCAATGG ACTTGCTACC GATGCTGATC CAGACAGTAG AAAAAGCAGC ATCTCAAAGT 
ACTCAGATTC CCATGGTAAC AGAAGGAGTT GCTGCAGCTT TGTTGATCTG CAGGATGTCT 
GTAATTGAGG GACAAACTGA GTCTAAGCTG AGTGGCTTCT GGCAGCTGAT CCTAGATGAG 
AAAAAACAAA TTTTTACCTC TGAGAAGTTC TTGCAGTCTG CTTCAGAGGA AGCAATGTGC 
ACTGTGCTGC AGCTGACAGA GCGCCTCCTC TTGGACCATG CACAGCGGCT CCCCGAGAGT 
AAAATCCAGC AGTATCACCG TGCCCTTGTT GCAGTGCTGC TGAGTCGGAC CTGGCACGTT 
CGAAGACAGG CCCATCAGAC AGTTAGGAAG CTTTTGTCCT CTCTGGGAGG GTACAAACTG 
GCCTACGGGC TCTTAGAGGA GCTAAAAGTG GTTCTCAGCT CTCATAAGGT TATGCCTCAA 
GAAGTCCTGG TGATGGAGTC AGGTGAGCTG TCAGAACAAG GGAAGACTTA TATTCCTCCC 
CGTGTCCTTC ATGAGGCACT GTGTGTCATC TCAAGTGTAG CTGGGCTGGA TGTGGATTCC 
TTTGAGACAG AAAAGCTGGC TCTGGAGATG CTGCTGGTGA GCCATCATCC ATCTCTGGTG 
GCAATACAGC CAGGTCTTTG GCCAGCCCTC CTTGTCAAGA TGAAGATAGA TCCCAAAGAC 
TTTATTACTA AACACCTGAA TGACATATTA CCCAGACTCA CTGCCTACTC TCCAGACAAC 
CAGTCATCTA TGAATGCAGT GGGATCTCTC TCTCTGCTCT CACCTGGTAG AGTGCTCCCA 
CAGCTCATCA GCACCATCTC AGCATCAATG CAGAATCCAG CTCTGTGCCA GGTTACTCGA 
GAGGAATTTG CCATCATGAA GACACCGGAG GGAGAACTGT ATGATAAGTC TATAATACAG 
AGTGCTCAAC AGGATAGTAT GAAAAAGGCT AACATGAAGA GGGAGAATAA AGCCTACTCC 
TTCAAGGAAC AAATCATTGA GCTGGAGCTG AAGGAGGAGA TAAAGAAGAA GAAGGGGATA 
AAGGATGAGG TGCAGCTGAC TAGCAAGCAG AAAGAGCTGA TGCATGCACA GCTGGAAAAG 
GAATCTCAGA TAAGAAAACG ACTCAAGGAG CTTGATAATG AACTGGAAAC AGCCTTGGGA 
CTGCTGGACA CTGTTATCAA GAGAAAACCT CCTGGTCTAA CTCAGTACAT CCCTAGTCTT 
GTTGGTTCCT TTTTGCCACT TTTCAGATCG CCTTTGGCTG CTCCAAGAAT TAAGGATCCT 
TTTCTTTCCT TAGCTTCCTG TGTCATGCCT GCTCGACTTA AAACCTTTGG TACTTTAGTG 
AGCCACGTGA CCTTGCGCCT GATGAAACCA GAATGTGAGT TAGATGAATC GTGGTGTCAA 
GAAGAGCTTC CTACTGCTCT CAACAGAGTA GTAAGCTTGC TGCATGCCCA CACCATTCCT 
AGCCGAACTG GCAAAGGAGA GGCAGGTGCT GCCCCATTAT CAGCTCCAGC ATTTTCCCTA 
GTCTTTCCTC TCCTAAAGAC GGCTATGACT GAGACATCCC ATGACAGTGA AGATAAGGAG 
GAATTCCTGG TCAAGATTCT GCAGATCCTT ACAGTTCATG CTCAGCTGAG ATCATCCACA 
AACGGCCAGG CTTTGCTGGT GGATGAGAAT GGCCCGGAGT TGCTGCCCCG ACGGGACATG 
CTGCTTCTGC TGACCCGAGT GATAGCAACA GGCTCTCCAC GGTTACAGGT CTTGGCTTCC 
AATGCACTGA CCTCTGTGTG CACAAGTAGC AGTGGAGAGG ATGGGTGTGC CTATGCAGAA 
CAAGAGGAGA TTGATGTGTT ACTTCAGGCC CTGCAGTCTC CATGCATGAA TGTTCGAGAT 
GCAGCTCTCA GGGGACTGAT GGAGCTACAG ATGGTTCTAC CAACCCCAGA CAGCGATGAA 
AAGAATGGAT TAAATCTGTT GCGTCGCCTT TGGGTAGTAA AGTTTGATGT GGAAGATGAG 
ATTCAGAAGC TGGCAGAGAG GTTGTGGGAG TCCATGGGTC TGGAACTACA GCCTGACCTC 
TGTTCTCTGC TGATCAAGGA TGTCATCTAC CATGAAGAGG CAGTGCGACA AGCAGGTGCC 
GAAGCTCTTT CCAAAGCTGT AGCTCAGTAT CAGCACCAGG CAGCAGAGGT TATGAATAAA 
CTTACAGAGA TTTACCAGGA GAAGCTCTAT AGGCCACCTC CAGTTTTGGA TGCTTTGGGA 
CGAGTGATAT CTGAATCACC ACCTGACCAG TGGGAAGCAA GGTGTGGCAT TGCTTTGGCC 
CTTAACAAGC TCTCAGAGCA TCTGGACAGT TCTCAGGTGA AGCCACTCTT TCAGTTTTTT 
GTACCAGATG CTCTGAATGA TCGCAATCCT GAAGTCAGGA AGTGCATGTT AGATGCAGCT 
CTTTCAACGC TCAACACTCA TGGCAAAGAC AATGTTAACT CTCTCTTGCC AGTGTTCGAA 
GAGTTCCTGA AAAATGCTCC TAATGATGCC AGTTATGATG CTGTCAGGCA GAGTGTGGTC 
ATTCTGATGG GCTCACTGGC AAAACATCTG GACAAAAGTG ACCCTAAAGT GAAACCAATT 
GTTGGCAAAC TGATTGCAGC CTTGTCCACA CCATCTCAGC AGGTTCAAGA GTCAGTGGCA 
AGCTGTCTTC CACCACTGGT GCCTGCAATT AAGGAGGATG CAGGAGGAAT GATACAGAAG 
CTAATGCAGC TGCTTTTAGA ATCTGATAAA TATGCCGAGC GCAAAGGTGC AGCTTATGGA 
CTGGCAGGTC TGGTGAAAGG CCTTGGCATC CTTTCTCTCA AGCAGCAGGA GATGATGACC 
ACACTGACTG ATGCTATCCA AGACAAGAAG AACTTTCGTC GGCGGGAGGG GGCTCTATTT 
GCCTTTGAGA TGCTCTGCAG CATGCTTGGA AAGCTCTTTG AGCCCTATGT GGTTCATGTG 
CTACCTCACT TGTTGCTTTG TTTTGGAGAT GGGAACCAGT ATGTGCGAGA GGCTGCAGAT 
GACTGTGCCA AAGCAGTGAT GAGCAACTTG AGTACTCATG GAGTGAAGCT GGTTTTGCCA 
TCACTTCTTG CTGCACTAGA AGAGGAATCT TGGAGAACCA AAGCTGGGTC AGTGGAACTG 
CTGGGAGCTA TGGCATACTG TGCTCCCAAA CAGCTCTCCT CCTGCTTACC CAATATTGTG 
CCAAAACTTA CTGAAGTGCT CACAGATTCT CATGTGAAGG TGCAGAATGC AGGTCAGCAA 
GCTCTCAGGC AGATTGGGTC CGTCATCAGA AATCCAGAGA TCCTTGCAAT TGCTCCAGTT 
TTGTTGGATG CTCTCACTGA CCCCTCCAGG AAGACCCAAA AGTGTCTGCA GACTCTACTG 
GATACCAAAT TTGTCCACTT CATTGATGCT CCATCCTTAG CACTTATTAT GCCAATTGTT 
CAGAGAGCTT TTCAGGATCG TTCCACAGAT ACCAGGAAAA TGGCTGCCCA GATTATTGGG 
AACATGTACT CCTTGACTGA TCAGAAGGAT CTGGCTCCTT ACCTACCCAG TGTGACTCCT 
GGACTGAAGG CATCCCTGCT GGATCCTGTA CCTGAAGTGC GTACAGTATC TGCAAAGGCA 
CTGGGAGCCA TGGTGAAGGG CATGGGAGAA TCATGCTTTG AGGATTTGTT GCCATGGCTG 
ATGGAGACAC TGACATACGA GCAAAGCTCA GTGGACCGAT CTGGTGCAGC TCAAGGTCTG 
GCTGAAGTTA TGGCTGGTTT AGGGGTGGAG AAGCTGGAGA AACTTATGCC AGAGATTGTA 
GCTACTGCCA GTAAGGTGGA TATTGCTCCA CATGTACGAG ATGGTTATAT AATGATGTTT 
AACTACCTGC CAATTACTTT TGGAGACAAG TTCATTCCGT ATGTTGGTCC CATCATCCCT 
TGTATCCTTA AGGCACTGGC AGATGAGAAT GAGTTTGTGC GGGACACTGC CCTGCGTGCT 
GGCCAGAGGA TCATAAGTAT GTATGCAGAG ACAGCCATTG CACTGCTTCT GCCACAGCTT 
GAGGATGGCC TGTTTGATGA CCTGTGGAGG ATAAGGTTTA GCTCAGTTCA GCTCCTTGGA 
GATCTTTTAT TCCATATCTC AGGAGTCACT GGAAAAATGA CCACAGAAAC TGCCTCAGAA 
GATGACAACT TTGGAACAGC CCAGTCAAAC AAGGCTATTA TTAATGCTCT TGGGGTGGAG 
AGGAGGAACA GGGTGCTGGC AGGGCTGTAC ATGGGCCGCT CTGACACCCA GCTGGTAGTG 
CGTCAAGCTT CGTTACACGT TTGGAAGATT GTAGTTTCAA ACACTCCTCG CACGCTGCGT 
GAAATTTTGC CAACACTCTT TGGGCTTCTG CTGAAATTCT TGGCCAGTAC TTGTGCAGAT 
AAGCGAACGG TTGCAGCACG GACATTAGGA GACCTTGTCC GAAAGTTAGG AGAGAAGATC 
CTTCCTGAAA TCATTCCCAT TCTGGAAGAT GGCCTAAGGT CAGATAAGAA TGATGAAAGG 
CAAGGAGTCT GCATTGGGCT GAGTGAGATA ATGAAGTCTA CCAGTAGAGA TGCGGTACTG 
GTTTTCTCTG AGTCCCTAGT GCCTACAGTG AGAAAAGCAT TGTGTGACCC TCTGGAAGAA 
GTGAGAGAGG CTGCAGCTAA AACATTTGAA CAGTTGCACT CAACAATTGG GCATCAGGCT 
CTTGAAGATA TCCTTCCGTT TTTGTTGAAG CAGCTGGATA ATGAAGAAAC ATCTGACTTT 
GCTGTGGATG GTCTTAAACA AGTTATGGCT GTCAAGAGCC GTGTGGTACT GCCTTATTTG 
GTGCCAAAGC TGACTACTCC CCCTGTGAAC ACCCGTGTGC TAGCTTTCCT CTCATCAGTG 
GCAGGGGATG CTCTGACAAG GCACTTGAGT GTCATCCTGC CTGCTATGAT GTCTGCACTG 
AAAGAGAAGC TGGGGACTAG TGAAGAGCAA CTGGAAATGG CAAATTGCCA GTCTGTAATC 
CTTTCTGTGG AAGATGATGT TGGACAAAGA ATTATAACTG AGGATTTACT AGAAGCTACC 
CGCAGCCCTG ACGTGGGAAT GAGACAAGCA GCAGCTATCA TTCTGAATAT CTACTGCTCC 
AAGACAAAAG CAGACTACAC TGGTCATCTC AAGAACTTGG TTTCAGGCCT GATACGTTTA 
TTTAATGATA CCAATCCTGT AGTTTTGAAT GAAAGCTGGG ATGCACTTAA TTCCATCACC 
AAGAAATTAG ATGCTGGGAA CCAGCTTGCT CTCATTGAGG ACCTACACAA GGACATCCGG 
GTTGTAGGGA ATGAGGCCAA AGGCGAGCAT GTGCCAGGAT TTTGTATTCC TAAGAAGGGA 
GTGACTTCTA TACTTCTGGT GCTGCGTGAA GGTGTTCTAA CTGGTAATCC AGAGCAAAAG 
GAGGAAGCAG CAAAGGCCTT GGGGCTGGTT ATTAAGCTGA CTTCTGCGGA AGCTCTTAAA 
CCATCTGTAG TGAGCATTAC TGGGCCACTT ATTCGTATCT TGGGAGATCG GTTCAGCTGG 
AATGTCAAAG TGGCTCTGCT TGAAACACTA AGCCTTCTTT TAGCCAAGGT TGAGATTGCC 
CTGAAACCAT TTTTGCCTCA ACTGCAAACA ACCTTCACCA AAGCTCTGCA AGATTCAAAC 
CGTGCTGTTC GCCTTAAAGC TGCTGATGCT CTTGGTAAAC TTATTGTTAT CCATGTAAAA 
GTGGATCCTC TTTTTACTGA GCTGCTGAAT GGAATTCGTT CCAGTGATGA CTCTGCCATC 
AGGGACACGA TGCTGCAAGC ACTGCGGTTT GTAACACGAG GAGCTGGTGC AAAAGTAGAT 
GCCACAATTA GGAAGAACAT CAGCACAGTT CTTCTTGGGA TGCTGGGACA CGATGAGGAT 
GCCACCCGCA TGGCTTCAGC TGGCTGCTTA GCAGAACTGT GTGCATTTCT GTCAGAAGAG 
GAGCTCAATA CTGTTCTCCA CCAGCACTTA CTGGCAGACA TCTCTGGAAT TGACTGGATG 
GTGAGACATG GACGAAGCCT GGCCCTCTCA GTGGCTGTGA ATGTTGCTCC TTATAGGCTG 
TGTTCCCCCA AGTACTATAA CAGTGTTCTG GAGATGATCC TCAGCAATGC CACTGCTGAC 
AGGATACCCA TTGCAGTTAG TGGCATCAGA GGGATGGGCT TTCTTATGAA ATACCACATG 
GAAGAAGGAG GGAACCTGCC TCCAAAGCTT TCCAACCTCT TTATTAAGTG TCTCCAGAAC 
TCATCCAGTG ACATAAAGTT AGTTGCAGAA AAAATGATCT GGTGGGCAAA TAAAAACCAT 
CTTCCTGCAC TGGATCCTCA GACAATTAAA CCAATTCTCA AAGCGCTTCT GGACAACACA 
AAAGACAAAA ACACCAGTGT AAGAGCTTAC AGCGACCAAG CCATTGTTAA TCTTCTGAAG 
ATGAGAGTGG GTGAACAAGT GCTTGAGTCT GTTTCCAAGA TTTTAGATGC TGCCAGCTTG 
GAGCTTCTCA ATGAAAGCTG CAGGAGATCT CTTAAGAAGC TGGCTGGCCA GGCTGACTCT 
GATGAACAGA TAGATGACAC TATACTGACG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCN1 ( XM_015275398.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015130884.1 Gallus gallus GCN1, eIF2 alpha kinase activator homolog (GCN1), transcript variant X2, mRNA.

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CloneID	OGa42823
Clone ID Related Accession (Same CDS sequence)	XM_015275398.2 , XM_015275398.1
Accession Version	XM_015275398.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	eIF-2-alpha kinase activator GCN1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006102.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015275398.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG ACACGCAGAT ATCTGACACA CTGAAGCGGT TTGCAGTGAA GGTAACTACA 
GCCAGCGTGA AGGAGCGGAG GGAGATCCTC AGTGAGCTGG GAAAATGCAT TTCTGGGAAA 
GATCTTCCCG AGGGTGCAGT GAAAGGCCTT TGCAAACTCT TCTGCCTTAC TCTGCACCGA 
TACCGCGACG CGGCGTCGCG CAGAGCCCTG CAGTTGGCGC TTCAGCAGCT CGCGGAGTCC 
CAGCCAGATG CAACAGCAAA AAACCTTCTG CAGTCACTTC AGTCTTCGGG GATCAGTGGC 
AAGGCTGGAG CTCCCAGTAA GAGCAGTGGA TCTGCAGCCC TGCTGGCACT CTCCTGGACA 
TGCCTGCTGG TACGGACAGT CTTTCCATCA CCTGACAAGA GACAGGGAGA AACCTGGAAA 
AAACTGGTGG AGGTTCAATG TTTACTTCTT TTGGAAGTCT TGGGAGGCTC TCATAAGCAG 
GCAGTGGTTG GAGCAGTGAA GAAACTGAAC CGACTTTGGA AAGAGAACCA AGATCTGGTG 
GATCAGTACC TATCTGTCAT TCTTGGTCTT GAACCAAACC AGAGCTATGC TGCAATGCTG 
GGACTTGTGG TGCAGTTTTG CACAGCTCAG AAAGAGACTG ATATTATTAA ACGCCACAAG 
AGTGCTCTTC TGGATTTCTA TGTGAAGACC ATCCTGATGA GCAAGACAAA GCCTCAGAAG 
CATGTGCTGG ACAGCTGCTC TCCACTTCTT CGATATATCT CTCACGCTGA ATTCAAAGAC 
TTACTGTTGC CAACTCTGCA GAAATCCTTG CTACGGAGCC CTGAGAATGT AATTGAAACA 
ATCTCTTGCT TGCTCTTGTC TGTTAACCTG GATCTTAGCC AGTATGCTCT GGACATTGTG 
AAAGGACTGG CTGGTCATCT GAAATCCAAC AGTGTGCAGC TCATGGATGA AGCAGTTGTC 
GCATTAAAGA ATTTGGCTCG ACAGTGTAGT GATCCTTCAG CCGTGGAATC CCTGAGCAGA 
CACCTTTTTG CCATCTTAGG GGGCTCAGAG GGGAAGCTGA CAGTTGTTGC GCAGAAGATG 
AGCGTCCTTT CAGGGATTGG CAGCCTTAGT CACCATGTGG TTTCTGGATC ATCTAGCCAA 
GCTCTGGCTG GAACCGTGGC TGAGCTTTTC ATCCCTTTTC TGCAGCAAGA AGTCCACGAA 
GGCACGTTGG TTCATGCAGT CTCTGTCCTG GCTCTGTGGT GCGTTCGCTT CACCACTGAA 
GTCCCTAAGA AGATGGTGGA ATGGCTAAAG AAAGCATTTG GCCTTAAAAC CTCTACTTCA 
GCTGTGAGGC ATGCCTACCT GCAGTGCATG CTAGCCTCTT TCAAAGGTGA CACGTTATTA 
CAGGCAATGG ACTTGCTACC GATGCTGATC CAGACAGTAG AAAAAGCAGC ATCTCAAAGT 
ACTCAGATTC CCATGGTAAC AGAAGGAGTT GCTGCAGCTT TGTTGATCTG CAGGATGTCT 
GTAATTGAGG GACAAACTGA GTCTAAGCTG AGTGGCTTCT GGCAGCTGAT CCTAGATGAG 
AAAAAACAAA TTTTTACCTC TGAGAAGTTC TTGCAGTCTG CTTCAGAGGA AGCAATGTGC 
ACTGTGCTGC AGCTGACAGA GCGCCTCCTC TTGGACCATG CACAGCGGCT CCCCGAGAGT 
AAAATCCAGC AGTATCACCG TGCCCTTGTT GCAGTGCTGC TGAGTCGGAC CTGGCACGTT 
CGAAGACAGG CCCATCAGAC AGTTAGGAAG CTTTTGTCCT CTCTGGGAGG GTACAAACTG 
GCCTACGGGC TCTTAGAGGA GCTAAAAGTG GTTCTCAGCT CTCATAAGGT TATGCCTCAA 
GAAGTCCTGG TGATGGAGTC AGGTGAGCTG TCAGAACAAG GGAAGACTTA TATTCCTCCC 
CGTGTCCTTC ATGAGGCACT GTGTGTCATC TCAAGTGTAG CTGGGCTGGA TGTGGATTCC 
TTTGAGACAG AAAAGCTGGC TCTGGAGATG CTGCTGGTGA GCCATCATCC ATCTCTGGTG 
GCAATACAGC CAGGTCTTTG GCCAGCCCTC CTTGTCAAGA TGAAGATAGA TCCCAAAGAC 
TTTATTACTA AACACCTGAA TGACATATTA CCCAGACTCA CTGCCTACTC TCCAGACAAC 
CAGTCATCTA TGAATGCAGT GGGATCTCTC TCTCTGCTCT CACCTGGTAG AGTGCTCCCA 
CAGCTCATCA GCACCATCTC AGCATCAATG CAGAATCCAG CTCTGTGCCA GGTTACTCGA 
GAGGAATTTG CCATCATGAA GACACCGGAG GGAGAACTGT ATGATAAGTC TATAATACAG 
AGTGCTCAAC AGGATAGTAT GAAAAAGGCT AACATGAAGA GGGAGAATAA AGCCTACTCC 
TTCAAGGAAC AAATCATTGA GCTGGAGCTG AAGGAGGAGA TAAAGAAGAA GAAGGGGATA 
AAGGATGAGG TGCAGCTGAC TAGCAAGCAG AAAGAGCTGA TGCATGCACA GCTGGAAAAG 
GAATCTCAGA TAAGAAAACG ACTCAAGGAG CTTGATAATG AACTGGAAAC AGCCTTGGGA 
CTGCTGGACA CTGTTATCAA GAGAAAACCT CCTGGTCTAA CTCAGTACAT CCCTAGTCTT 
GTTGGTTCCT TTTTGCCACT TTTCAGATCG CCTTTGGCTG CTCCAAGAAT TAAGGATCCT 
TTTCTTTCCT TAGCTTCCTG TGTCATGCCT GCTCGACTTA AAACCTTTGG TACTTTAGTG 
AGCCACGTGA CCTTGCGCCT GATGAAACCA GAATGTGAGT TAGATGAATC GTGGTGTCAA 
GAAGAGCTTC CTACTGCTCT CAACAGAGTA GTAAGCTTGC TGCATGCCCA CACCATTCCT 
AGCCGAACTG GCAAAGGAGA GGCAGGTGCT GCCCCATTAT CAGCTCCAGC ATTTTCCCTA 
GTCTTTCCTC TCCTAAAGAC GGCTATGACT GAGACATCCC ATGACAGTGA AGATAAGGAG 
GAATTCCTGG TCAAGATTCT GCAGATCCTT ACAGTTCATG CTCAGCTGAG ATCATCCACA 
AACGGCCAGG CTTTGCTGGT GGATGAGAAT GGCCCGGAGT TGCTGCCCCG ACGGGACATG 
CTGCTTCTGC TGACCCGAGT GATAGCAACA GGCTCTCCAC GGTTACAGGT CTTGGCTTCC 
AATGCACTGA CCTCTGTGTG CACAAGTAGC AGTGGAGAGG ATGGGTGTGC CTATGCAGAA 
CAAGAGGAGA TTGATGTGTT ACTTCAGGCC CTGCAGTCTC CATGCATGAA TGTTCGAGAT 
GCAGCTCTCA GGGGACTGAT GGAGCTACAG ATGGTTCTAC CAACCCCAGA CAGCGATGAA 
AAGAATGGAT TAAATCTGTT GCGTCGCCTT TGGGTAGTAA AGTTTGATGT GGAAGATGAG 
ATTCAGAAGC TGGCAGAGAG GTTGTGGGAG TCCATGGGTC TGGAACTACA GCCTGACCTC 
TGTTCTCTGC TGATCAAGGA TGTCATCTAC CATGAAGAGG CAGTGCGACA AGCAGGTGCC 
GAAGCTCTTT CCAAAGCTGT AGCTCAGTAT CAGCACCAGG CAGCAGAGGT TATGAATAAA 
CTTACAGAGA TTTACCAGGA GAAGCTCTAT AGGCCACCTC CAGTTTTGGA TGCTTTGGGA 
CGAGTGATAT CTGAATCACC ACCTGACCAG TGGGAAGCAA GGTGTGGCAT TGCTTTGGCC 
CTTAACAAGC TCTCAGAGCA TCTGGACAGT TCTCAGGTGA AGCCACTCTT TCAGTTTTTT 
GTACCAGATG CTCTGAATGA TCGCAATCCT GAAGTCAGGA AGTGCATGTT AGATGCAGCT 
CTTTCAACGC TCAACACTCA TGGCAAAGAC AATGTTAACT CTCTCTTGCC AGTGTTCGAA 
GAGTTCCTGA AAAATGCTCC TAATGATGCC AGTTATGATG CTGTCAGGCA GAGTGTGGTC 
ATTCTGATGG GCTCACTGGC AAAACATCTG GACAAAAGTG ACCCTAAAGT GAAACCAATT 
GTTGGCAAAC TGATTGCAGC CTTGTCCACA CCATCTCAGC AGGTTCAAGA GTCAGTGGCA 
AGCTGTCTTC CACCACTGGT GCCTGCAATT AAGGAGGATG CAGGAGGAAT GATACAGAAG 
CTAATGCAGC TGCTTTTAGA ATCTGATAAA TATGCCGAGC GCAAAGGTGC AGCTTATGGA 
CTGGCAGGTC TGGTGAAAGG CCTTGGCATC CTTTCTCTCA AGCAGCAGGA GATGATGACC 
ACACTGACTG ATGCTATCCA AGACAAGAAG AACTTTCGTC GGCGGGAGGG GGCTCTATTT 
GCCTTTGAGA TGCTCTGCAG CATGCTTGGA AAGCTCTTTG AGCCCTATGT GGTTCATGTG 
CTACCTCACT TGTTGCTTTG TTTTGGAGAT GGGAACCAGT ATGTGCGAGA GGCTGCAGAT 
GACTGTGCCA AAGCAGTGAT GAGCAACTTG AGTACTCATG GAGTGAAGCT GGTTTTGCCA 
TCACTTCTTG CTGCACTAGA AGAGGAATCT TGGAGAACCA AAGCTGGGTC AGTGGAACTG 
CTGGGAGCTA TGGCATACTG TGCTCCCAAA CAGCTCTCCT CCTGCTTACC CAATATTGTG 
CCAAAACTTA CTGAAGTGCT CACAGATTCT CATGTGAAGG TGCAGAATGC AGGTCAGCAA 
GCTCTCAGGC AGATTGGGTC CGTCATCAGA AATCCAGAGA TCCTTGCAAT TGCTCCAGTT 
TTGTTGGATG CTCTCACTGA CCCCTCCAGG AAGACCCAAA AGTGTCTGCA GACTCTACTG 
GATACCAAAT TTGTCCACTT CATTGATGCT CCATCCTTAG CACTTATTAT GCCAATTGTT 
CAGAGAGCTT TTCAGGATCG TTCCACAGAT ACCAGGAAAA TGGCTGCCCA GATTATTGGG 
AACATGTACT CCTTGACTGA TCAGAAGGAT CTGGCTCCTT ACCTACCCAG TGTGACTCCT 
GGACTGAAGG CATCCCTGCT GGATCCTGTA CCTGAAGTGC GTACAGTATC TGCAAAGGCA 
CTGGGAGCCA TGGTGAAGGG CATGGGAGAA TCATGCTTTG AGGATTTGTT GCCATGGCTG 
ATGGAGACAC TGACATACGA GCAAAGCTCA GTGGACCGAT CTGGTGCAGC TCAAGGTCTG 
GCTGAAGTTA TGGCTGGTTT AGGGGTGGAG AAGCTGGAGA AACTTATGCC AGAGATTGTA 
GCTACTGCCA GTAAGGTGGA TATTGCTCCA CATGTACGAG ATGGTTATAT AATGATGTTT 
AACTACCTGC CAATTACTTT TGGAGACAAG TTCATTCCGT ATGTTGGTCC CATCATCCCT 
TGTATCCTTA AGGCACTGGC AGATGAGAAT GAGTTTGTGC GGGACACTGC CCTGCGTGCT 
GGCCAGAGGA TCATAAGTAT GTATGCAGAG ACAGCCATTG CACTGCTTCT GCCACAGCTT 
GAGGATGGCC TGTTTGATGA CCTGTGGAGG ATAAGGTTTA GCTCAGTTCA GCTCCTTGGA 
GATCTTTTAT TCCATATCTC AGGAGTCACT GGAAAAATGA CCACAGAAAC TGCCTCAGAA 
GATGACAACT TTGGAACAGC CCAGTCAAAC AAGGCTATTA TTAATGCTCT TGGGGTGGAG 
AGGAGGAACA GGGTGCTGGC AGGGCTGTAC ATGGGCCGCT CTGACACCCA GCTGGTAGTG 
CGTCAAGCTT CGTTACACGT TTGGAAGATT GTAGTTTCAA ACACTCCTCG CACGCTGCGT 
GAAATTTTGC CAACACTCTT TGGGCTTCTG CTGAAATTCT TGGCCAGTAC TTGTGCAGAT 
AAGCGAACGG TTGCAGCACG GACATTAGGA GACCTTGTCC GAAAGTTAGG AGAGAAGATC 
CTTCCTGAAA TCATTCCCAT TCTGGAAGAT GGCCTAAGGT CAGATAAGAA TGATGAAAGG 
CAAGGAGTCT GCATTGGGCT GAGTGAGATA ATGAAGTCTA CCAGTAGAGA TGCGGTACTG 
GTTTTCTCTG AGTCCCTAGT GCCTACAGTG AGAAAAGCAT TGTGTGACCC TCTGGAAGAA 
GTGAGAGAGG CTGCAGCTAA AACATTTGAA CAGTTGCACT CAACAATTGG GCATCAGGCT 
CTTGAAGATA TCCTTCCGTT TTTGTTGAAG CAGCTGGATA ATGAAGAAAC ATCTGACTTT 
GCTGTGGATG GTCTTAAACA AGTTATGGCT GTCAAGAGCC GTGTGGTACT GCCTTATTTG 
GTGCCAAAGC TGACTACTCC CCCTGTGAAC ACCCGTGTGC TAGCTTTCCT CTCATCAGTG 
GCAGGGGATG CTCTGACAAG GCACTTGAGT GTCATCCTGC CTGCTATGAT GTCTGCACTG 
AAAGAGAAGC TGGGGACTAG TGAAGAGCAA CTGGAAATGG CAAATTGCCA GTCTGTAATC 
CTTTCTGTGG AAGATGATGT TGGACAAAGA ATTATAACTG AGGATTTACT AGAAGCTACC 
CGCAGCCCTG ACGTGGGAAT GAGACAAGCA GCAGCTATCA TTCTGAATAT CTACTGCTCC 
AAGACAAAAG CAGACTACAC TGGTCATCTC AAGAACTTGG TTTCAGGCCT GATACGTTTA 
TTTAATGATA CCAATCCTGT AGTTTTGAAT GAAAGCTGGG ATGCACTTAA TTCCATCACC 
AAGAAATTAG ATGCTGGGAA CCAGCTTGCT CTCATTGAGG ACCTACACAA GGACATCCGG 
GTTGTAGGGA ATGAGGCCAA AGGCGAGCAT GTGCCAGGAT TTTGTATTCC TAAGAAGGGA 
GTGACTTCTA TACTTCTGGT GCTGCGTGAA GGTGTTCTAA CTGGTAATCC AGAGCAAAAG 
GAGGAAGCAG CAAAGGCCTT GGGGCTGGTT ATTAAGCTGA CTTCTGCGGA AGCTCTTAAA 
CCATCTGTAG TGAGCATTAC TGGGCCACTT ATTCGTATCT TGGGAGATCG GTTCAGCTGG 
AATGTCAAAG TGGCTCTGCT TGAAACACTA AGCCTTCTTT TAGCCAAGGT TGAGATTGCC 
CTGAAACCAT TTTTGCCTCA ACTGCAAACA ACCTTCACCA AAGCTCTGCA AGATTCAAAC 
CGTGCTGTTC GCCTTAAAGC TGCTGATGCT CTTGGTAAAC TTATTGTTAT CCATGTAAAA 
GTGGATCCTC TTTTTACTGA GCTGCTGAAT GGAATTCGTT CCAGTGATGA CTCTGCCATC 
AGGGACACGA TGCTGCAAGC ACTGCGGTTT GTAACACGAG GAGCTGGTGC AAAAGTAGAT 
GCCACAATTA GGAAGAACAT CAGCACAGTT CTTCTTGGGA TGCTGGGACA CGATGAGGAT 
GCCACCCGCA TGGCTTCAGC TGGCTGCTTA GCAGAACTGT GTGCATTTCT GTCAGAAGAG 
GAGCTCAATA CTGTTCTCCA CCAGCACTTA CTGGACATCT CTGGAATTGA CTGGATGGTG 
AGACATGGAC GAAGCCTGGC CCTCTCAGTG GCTGTGAATG TTGCTCCTTA TAGGCTGTGT 
TCCCCCAAGT ACTATAACAG TGTTCTGGAG ATGATCCTCA GCAATGCCAC TGCTGACAGG 
ATACCCATTG CAGTTAGTGG CATCAGAGGG ATGGGCTTTC TTATGAAATA CCACATGGAA 
GAAGGAGGGA ACCTGCCTCC AAAGCTTTCC AACCTCTTTA TTAAGTGTCT CCAGAACTCA 
TCCAGTGACA TAAAGTTAGT TGCAGAAAAA ATGATCTGGT GGGCAAATAA AAACCATCTT 
CCTGCACTGG ATCCTCAGAC AATTAAACCA ATTCTCAAAG CGCTTCTGGA CAACACAAAA 
GACAAAAACA CCAGTGTAAG AGCTTACAGC GACCAAGCCA TTGTTAATCT TCTGAAGATG 
AGAGTGGGTG AACAAGTGCT TGAGTCTGTT TCCAAGATTT TAGATGCTGC CAGCTTGGAG 
CTTCTCAATG AAAGCTGCAG GAGATCTCTT AAGAAGCTGG CTGGCCAGGC TGACTCTGAT 
GAACAGATAG ATGACACTAT ACTGACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015130884.1
CDS	61..8070
Translation	MAADTQISDTLKRFAVKVTTASVKERREILSELGKCISGKDLPEGAVKGLCKLFCLTLHRYRDAASRRALQLALQQLAESQPDATAKNLLQSLQSSGISGKAGAPSKSSGSAALLALSWTCLLVRTVFPSPDKRQGETWKKLVEVQCLLLLEVLGGSHKQAVVGAVKKLNRLWKENQDLVDQYLSVILGLEPNQSYAAMLGLVVQFCTAQKETDIIKRHKSALLDFYVKTILMSKTKPQKHVLDSCSPLLRYISHAEFKDLLLPTLQKSLLRSPENVIETISCLLLSVNLDLSQYALDIVKGLAGHLKSNSVQLMDEAVVALKNLARQCSDPSAVESLSRHLFAILGGSEGKLTVVAQKMSVLSGIGSLSHHVVSGSSSQALAGTVAELFIPFLQQEVHEGTLVHAVSVLALWCVRFTTEVPKKMVEWLKKAFGLKTSTSAVRHAYLQCMLASFKGDTLLQAMDLLPMLIQTVEKAASQSTQIPMVTEGVAAALLICRMSVIEGQTESKLSGFWQLILDEKKQIFTSEKFLQSASEEAMCTVLQLTERLLLDHAQRLPESKIQQYHRALVAVLLSRTWHVRRQAHQTVRKLLSSLGGYKLAYGLLEELKVVLSSHKVMPQEVLVMESGELSEQGKTYIPPRVLHEALCVISSVAGLDVDSFETEKLALEMLLVSHHPSLVAIQPGLWPALLVKMKIDPKDFITKHLNDILPRLTAYSPDNQSSMNAVGSLSLLSPGRVLPQLISTISASMQNPALCQVTREEFAIMKTPEGELYDKSIIQSAQQDSMKKANMKRENKAYSFKEQIIELELKEEIKKKKGIKDEVQLTSKQKELMHAQLEKESQIRKRLKELDNELETALGLLDTVIKRKPPGLTQYIPSLVGSFLPLFRSPLAAPRIKDPFLSLASCVMPARLKTFGTLVSHVTLRLMKPECELDESWCQEELPTALNRVVSLLHAHTIPSRTGKGEAGAAPLSAPAFSLVFPLLKTAMTETSHDSEDKEEFLVKILQILTVHAQLRSSTNGQALLVDENGPELLPRRDMLLLLTRVIATGSPRLQVLASNALTSVCTSSSGEDGCAYAEQEEIDVLLQALQSPCMNVRDAALRGLMELQMVLPTPDSDEKNGLNLLRRLWVVKFDVEDEIQKLAERLWESMGLELQPDLCSLLIKDVIYHEEAVRQAGAEALSKAVAQYQHQAAEVMNKLTEIYQEKLYRPPPVLDALGRVISESPPDQWEARCGIALALNKLSEHLDSSQVKPLFQFFVPDALNDRNPEVRKCMLDAALSTLNTHGKDNVNSLLPVFEEFLKNAPNDASYDAVRQSVVILMGSLAKHLDKSDPKVKPIVGKLIAALSTPSQQVQESVASCLPPLVPAIKEDAGGMIQKLMQLLLESDKYAERKGAAYGLAGLVKGLGILSLKQQEMMTTLTDAIQDKKNFRRREGALFAFEMLCSMLGKLFEPYVVHVLPHLLLCFGDGNQYVREAADDCAKAVMSNLSTHGVKLVLPSLLAALEEESWRTKAGSVELLGAMAYCAPKQLSSCLPNIVPKLTEVLTDSHVKVQNAGQQALRQIGSVIRNPEILAIAPVLLDALTDPSRKTQKCLQTLLDTKFVHFIDAPSLALIMPIVQRAFQDRSTDTRKMAAQIIGNMYSLTDQKDLAPYLPSVTPGLKASLLDPVPEVRTVSAKALGAMVKGMGESCFEDLLPWLMETLTYEQSSVDRSGAAQGLAEVMAGLGVEKLEKLMPEIVATASKVDIAPHVRDGYIMMFNYLPITFGDKFIPYVGPIIPCILKALADENEFVRDTALRAGQRIISMYAETAIALLLPQLEDGLFDDLWRIRFSSVQLLGDLLFHISGVTGKMTTETASEDDNFGTAQSNKAIINALGVERRNRVLAGLYMGRSDTQLVVRQASLHVWKIVVSNTPRTLREILPTLFGLLLKFLASTCADKRTVAARTLGDLVRKLGEKILPEIIPILEDGLRSDKNDERQGVCIGLSEIMKSTSRDAVLVFSESLVPTVRKALCDPLEEVREAAAKTFEQLHSTIGHQALEDILPFLLKQLDNEETSDFAVDGLKQVMAVKSRVVLPYLVPKLTTPPVNTRVLAFLSSVAGDALTRHLSVILPAMMSALKEKLGTSEEQLEMANCQSVILSVEDDVGQRIITEDLLEATRSPDVGMRQAAAIILNIYCSKTKADYTGHLKNLVSGLIRLFNDTNPVVLNESWDALNSITKKLDAGNQLALIEDLHKDIRVVGNEAKGEHVPGFCIPKKGVTSILLVLREGVLTGNPEQKEEAAKALGLVIKLTSAEALKPSVVSITGPLIRILGDRFSWNVKVALLETLSLLLAKVEIALKPFLPQLQTTFTKALQDSNRAVRLKAADALGKLIVIHVKVDPLFTELLNGIRSSDDSAIRDTMLQALRFVTRGAGAKVDATIRKNISTVLLGMLGHDEDATRMASAGCLAELCAFLSEEELNTVLHQHLLDISGIDWMVRHGRSLALSVAVNVAPYRLCSPKYYNSVLEMILSNATADRIPIAVSGIRGMGFLMKYHMEEGGNLPPKLSNLFIKCLQNSSSDIKLVAEKMIWWANKNHLPALDPQTIKPILKALLDNTKDKNTSVRAYSDQAIVNLLKMRVGEQVLESVSKILDAASLELLNESCRRSLKKLAGQADSDEQIDDTILT

Target ORF information:

RefSeq Version	XM_015275398.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus GCN1, eIF2 alpha kinase activator homolog (GCN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015275398.2

ORF Insert Sequence:

ATGGCGGCCG ACACGCAGAT ATCTGACACA CTGAAGCGGT TTGCAGTGAA GGTAACTACA 
GCCAGCGTGA AGGAGCGGAG GGAGATCCTC AGTGAGCTGG GAAAATGCAT TTCTGGGAAA 
GATCTTCCCG AGGGTGCAGT GAAAGGCCTT TGCAAACTCT TCTGCCTTAC TCTGCACCGA 
TACCGCGACG CGGCGTCGCG CAGAGCCCTG CAGTTGGCGC TTCAGCAGCT CGCGGAGTCC 
CAGCCAGATG CAACAGCAAA AAACCTTCTG CAGTCACTTC AGTCTTCGGG GATCAGTGGC 
AAGGCTGGAG CTCCCAGTAA GAGCAGTGGA TCTGCAGCCC TGCTGGCACT CTCCTGGACA 
TGCCTGCTGG TACGGACAGT CTTTCCATCA CCTGACAAGA GACAGGGAGA AACCTGGAAA 
AAACTGGTGG AGGTTCAATG TTTACTTCTT TTGGAAGTCT TGGGAGGCTC TCATAAGCAG 
GCAGTGGTTG GAGCAGTGAA GAAACTGAAC CGACTTTGGA AAGAGAACCA AGATCTGGTG 
GATCAGTACC TATCTGTCAT TCTTGGTCTT GAACCAAACC AGAGCTATGC TGCAATGCTG 
GGACTTGTGG TGCAGTTTTG CACAGCTCAG AAAGAGACTG ATATTATTAA ACGCCACAAG 
AGTGCTCTTC TGGATTTCTA TGTGAAGACC ATCCTGATGA GCAAGACAAA GCCTCAGAAG 
CATGTGCTGG ACAGCTGCTC TCCACTTCTT CGATATATCT CTCACGCTGA ATTCAAAGAC 
TTACTGTTGC CAACTCTGCA GAAATCCTTG CTACGGAGCC CTGAGAATGT AATTGAAACA 
ATCTCTTGCT TGCTCTTGTC TGTTAACCTG GATCTTAGCC AGTATGCTCT GGACATTGTG 
AAAGGACTGG CTGGTCATCT GAAATCCAAC AGTGTGCAGC TCATGGATGA AGCAGTTGTC 
GCATTAAAGA ATTTGGCTCG ACAGTGTAGT GATCCTTCAG CCGTGGAATC CCTGAGCAGA 
CACCTTTTTG CCATCTTAGG GGGCTCAGAG GGGAAGCTGA CAGTTGTTGC GCAGAAGATG 
AGCGTCCTTT CAGGGATTGG CAGCCTTAGT CACCATGTGG TTTCTGGATC ATCTAGCCAA 
GCTCTGGCTG GAACCGTGGC TGAGCTTTTC ATCCCTTTTC TGCAGCAAGA AGTCCACGAA 
GGCACGTTGG TTCATGCAGT CTCTGTCCTG GCTCTGTGGT GCGTTCGCTT CACCACTGAA 
GTCCCTAAGA AGATGGTGGA ATGGCTAAAG AAAGCATTTG GCCTTAAAAC CTCTACTTCA 
GCTGTGAGGC ATGCCTACCT GCAGTGCATG CTAGCCTCTT TCAAAGGTGA CACGTTATTA 
CAGGCAATGG ACTTGCTACC GATGCTGATC CAGACAGTAG AAAAAGCAGC ATCTCAAAGT 
ACTCAGATTC CCATGGTAAC AGAAGGAGTT GCTGCAGCTT TGTTGATCTG CAGGATGTCT 
GTAATTGAGG GACAAACTGA GTCTAAGCTG AGTGGCTTCT GGCAGCTGAT CCTAGATGAG 
AAAAAACAAA TTTTTACCTC TGAGAAGTTC TTGCAGTCTG CTTCAGAGGA AGCAATGTGC 
ACTGTGCTGC AGCTGACAGA GCGCCTCCTC TTGGACCATG CACAGCGGCT CCCCGAGAGT 
AAAATCCAGC AGTATCACCG TGCCCTTGTT GCAGTGCTGC TGAGTCGGAC CTGGCACGTT 
CGAAGACAGG CCCATCAGAC AGTTAGGAAG CTTTTGTCCT CTCTGGGAGG GTACAAACTG 
GCCTACGGGC TCTTAGAGGA GCTAAAAGTG GTTCTCAGCT CTCATAAGGT TATGCCTCAA 
GAAGTCCTGG TGATGGAGTC AGGTGAGCTG TCAGAACAAG GGAAGACTTA TATTCCTCCC 
CGTGTCCTTC ATGAGGCACT GTGTGTCATC TCAAGTGTAG CTGGGCTGGA TGTGGATTCC 
TTTGAGACAG AAAAGCTGGC TCTGGAGATG CTGCTGGTGA GCCATCATCC ATCTCTGGTG 
GCAATACAGC CAGGTCTTTG GCCAGCCCTC CTTGTCAAGA TGAAGATAGA TCCCAAAGAC 
TTTATTACTA AACACCTGAA TGACATATTA CCCAGACTCA CTGCCTACTC TCCAGACAAC 
CAGTCATCTA TGAATGCAGT GGGATCTCTC TCTCTGCTCT CACCTGGTAG AGTGCTCCCA 
CAGCTCATCA GCACCATCTC AGCATCAATG CAGAATCCAG CTCTGTGCCA GGTTACTCGA 
GAGGAATTTG CCATCATGAA GACACCGGAG GGAGAACTGT ATGATAAGTC TATAATACAG 
AGTGCTCAAC AGGATAGTAT GAAAAAGGCT AACATGAAGA GGGAGAATAA AGCCTACTCC 
TTCAAGGAAC AAATCATTGA GCTGGAGCTG AAGGAGGAGA TAAAGAAGAA GAAGGGGATA 
AAGGATGAGG TGCAGCTGAC TAGCAAGCAG AAAGAGCTGA TGCATGCACA GCTGGAAAAG 
GAATCTCAGA TAAGAAAACG ACTCAAGGAG CTTGATAATG AACTGGAAAC AGCCTTGGGA 
CTGCTGGACA CTGTTATCAA GAGAAAACCT CCTGGTCTAA CTCAGTACAT CCCTAGTCTT 
GTTGGTTCCT TTTTGCCACT TTTCAGATCG CCTTTGGCTG CTCCAAGAAT TAAGGATCCT 
TTTCTTTCCT TAGCTTCCTG TGTCATGCCT GCTCGACTTA AAACCTTTGG TACTTTAGTG 
AGCCACGTGA CCTTGCGCCT GATGAAACCA GAATGTGAGT TAGATGAATC GTGGTGTCAA 
GAAGAGCTTC CTACTGCTCT CAACAGAGTA GTAAGCTTGC TGCATGCCCA CACCATTCCT 
AGCCGAACTG GCAAAGGAGA GGCAGGTGCT GCCCCATTAT CAGCTCCAGC ATTTTCCCTA 
GTCTTTCCTC TCCTAAAGAC GGCTATGACT GAGACATCCC ATGACAGTGA AGATAAGGAG 
GAATTCCTGG TCAAGATTCT GCAGATCCTT ACAGTTCATG CTCAGCTGAG ATCATCCACA 
AACGGCCAGG CTTTGCTGGT GGATGAGAAT GGCCCGGAGT TGCTGCCCCG ACGGGACATG 
CTGCTTCTGC TGACCCGAGT GATAGCAACA GGCTCTCCAC GGTTACAGGT CTTGGCTTCC 
AATGCACTGA CCTCTGTGTG CACAAGTAGC AGTGGAGAGG ATGGGTGTGC CTATGCAGAA 
CAAGAGGAGA TTGATGTGTT ACTTCAGGCC CTGCAGTCTC CATGCATGAA TGTTCGAGAT 
GCAGCTCTCA GGGGACTGAT GGAGCTACAG ATGGTTCTAC CAACCCCAGA CAGCGATGAA 
AAGAATGGAT TAAATCTGTT GCGTCGCCTT TGGGTAGTAA AGTTTGATGT GGAAGATGAG 
ATTCAGAAGC TGGCAGAGAG GTTGTGGGAG TCCATGGGTC TGGAACTACA GCCTGACCTC 
TGTTCTCTGC TGATCAAGGA TGTCATCTAC CATGAAGAGG CAGTGCGACA AGCAGGTGCC 
GAAGCTCTTT CCAAAGCTGT AGCTCAGTAT CAGCACCAGG CAGCAGAGGT TATGAATAAA 
CTTACAGAGA TTTACCAGGA GAAGCTCTAT AGGCCACCTC CAGTTTTGGA TGCTTTGGGA 
CGAGTGATAT CTGAATCACC ACCTGACCAG TGGGAAGCAA GGTGTGGCAT TGCTTTGGCC 
CTTAACAAGC TCTCAGAGCA TCTGGACAGT TCTCAGGTGA AGCCACTCTT TCAGTTTTTT 
GTACCAGATG CTCTGAATGA TCGCAATCCT GAAGTCAGGA AGTGCATGTT AGATGCAGCT 
CTTTCAACGC TCAACACTCA TGGCAAAGAC AATGTTAACT CTCTCTTGCC AGTGTTCGAA 
GAGTTCCTGA AAAATGCTCC TAATGATGCC AGTTATGATG CTGTCAGGCA GAGTGTGGTC 
ATTCTGATGG GCTCACTGGC AAAACATCTG GACAAAAGTG ACCCTAAAGT GAAACCAATT 
GTTGGCAAAC TGATTGCAGC CTTGTCCACA CCATCTCAGC AGGTTCAAGA GTCAGTGGCA 
AGCTGTCTTC CACCACTGGT GCCTGCAATT AAGGAGGATG CAGGAGGAAT GATACAGAAG 
CTAATGCAGC TGCTTTTAGA ATCTGATAAA TATGCCGAGC GCAAAGGTGC AGCTTATGGA 
CTGGCAGGTC TGGTGAAAGG CCTTGGCATC CTTTCTCTCA AGCAGCAGGA GATGATGACC 
ACACTGACTG ATGCTATCCA AGACAAGAAG AACTTTCGTC GGCGGGAGGG GGCTCTATTT 
GCCTTTGAGA TGCTCTGCAG CATGCTTGGA AAGCTCTTTG AGCCCTATGT GGTTCATGTG 
CTACCTCACT TGTTGCTTTG TTTTGGAGAT GGGAACCAGT ATGTGCGAGA GGCTGCAGAT 
GACTGTGCCA AAGCAGTGAT GAGCAACTTG AGTACTCATG GAGTGAAGCT GGTTTTGCCA 
TCACTTCTTG CTGCACTAGA AGAGGAATCT TGGAGAACCA AAGCTGGGTC AGTGGAACTG 
CTGGGAGCTA TGGCATACTG TGCTCCCAAA CAGCTCTCCT CCTGCTTACC CAATATTGTG 
CCAAAACTTA CTGAAGTGCT CACAGATTCT CATGTGAAGG TGCAGAATGC AGGTCAGCAA 
GCTCTCAGGC AGATTGGGTC CGTCATCAGA AATCCAGAGA TCCTTGCAAT TGCTCCAGTT 
TTGTTGGATG CTCTCACTGA CCCCTCCAGG AAGACCCAAA AGTGTCTGCA GACTCTACTG 
GATACCAAAT TTGTCCACTT CATTGATGCT CCATCCTTAG CACTTATTAT GCCAATTGTT 
CAGAGAGCTT TTCAGGATCG TTCCACAGAT ACCAGGAAAA TGGCTGCCCA GATTATTGGG 
AACATGTACT CCTTGACTGA TCAGAAGGAT CTGGCTCCTT ACCTACCCAG TGTGACTCCT 
GGACTGAAGG CATCCCTGCT GGATCCTGTA CCTGAAGTGC GTACAGTATC TGCAAAGGCA 
CTGGGAGCCA TGGTGAAGGG CATGGGAGAA TCATGCTTTG AGGATTTGTT GCCATGGCTG 
ATGGAGACAC TGACATACGA GCAAAGCTCA GTGGACCGAT CTGGTGCAGC TCAAGGTCTG 
GCTGAAGTTA TGGCTGGTTT AGGGGTGGAG AAGCTGGAGA AACTTATGCC AGAGATTGTA 
GCTACTGCCA GTAAGGTGGA TATTGCTCCA CATGTACGAG ATGGTTATAT AATGATGTTT 
AACTACCTGC CAATTACTTT TGGAGACAAG TTCATTCCGT ATGTTGGTCC CATCATCCCT 
TGTATCCTTA AGGCACTGGC AGATGAGAAT GAGTTTGTGC GGGACACTGC CCTGCGTGCT 
GGCCAGAGGA TCATAAGTAT GTATGCAGAG ACAGCCATTG CACTGCTTCT GCCACAGCTT 
GAGGATGGCC TGTTTGATGA CCTGTGGAGG ATAAGGTTTA GCTCAGTTCA GCTCCTTGGA 
GATCTTTTAT TCCATATCTC AGGAGTCACT GGAAAAATGA CCACAGAAAC TGCCTCAGAA 
GATGACAACT TTGGAACAGC CCAGTCAAAC AAGGCTATTA TTAATGCTCT TGGGGTGGAG 
AGGAGGAACA GGGTGCTGGC AGGGCTGTAC ATGGGCCGCT CTGACACCCA GCTGGTAGTG 
CGTCAAGCTT CGTTACACGT TTGGAAGATT GTAGTTTCAA ACACTCCTCG CACGCTGCGT 
GAAATTTTGC CAACACTCTT TGGGCTTCTG CTGAAATTCT TGGCCAGTAC TTGTGCAGAT 
AAGCGAACGG TTGCAGCACG GACATTAGGA GACCTTGTCC GAAAGTTAGG AGAGAAGATC 
CTTCCTGAAA TCATTCCCAT TCTGGAAGAT GGCCTAAGGT CAGATAAGAA TGATGAAAGG 
CAAGGAGTCT GCATTGGGCT GAGTGAGATA ATGAAGTCTA CCAGTAGAGA TGCGGTACTG 
GTTTTCTCTG AGTCCCTAGT GCCTACAGTG AGAAAAGCAT TGTGTGACCC TCTGGAAGAA 
GTGAGAGAGG CTGCAGCTAA AACATTTGAA CAGTTGCACT CAACAATTGG GCATCAGGCT 
CTTGAAGATA TCCTTCCGTT TTTGTTGAAG CAGCTGGATA ATGAAGAAAC ATCTGACTTT 
GCTGTGGATG GTCTTAAACA AGTTATGGCT GTCAAGAGCC GTGTGGTACT GCCTTATTTG 
GTGCCAAAGC TGACTACTCC CCCTGTGAAC ACCCGTGTGC TAGCTTTCCT CTCATCAGTG 
GCAGGGGATG CTCTGACAAG GCACTTGAGT GTCATCCTGC CTGCTATGAT GTCTGCACTG 
AAAGAGAAGC TGGGGACTAG TGAAGAGCAA CTGGAAATGG CAAATTGCCA GTCTGTAATC 
CTTTCTGTGG AAGATGATGT TGGACAAAGA ATTATAACTG AGGATTTACT AGAAGCTACC 
CGCAGCCCTG ACGTGGGAAT GAGACAAGCA GCAGCTATCA TTCTGAATAT CTACTGCTCC 
AAGACAAAAG CAGACTACAC TGGTCATCTC AAGAACTTGG TTTCAGGCCT GATACGTTTA 
TTTAATGATA CCAATCCTGT AGTTTTGAAT GAAAGCTGGG ATGCACTTAA TTCCATCACC 
AAGAAATTAG ATGCTGGGAA CCAGCTTGCT CTCATTGAGG ACCTACACAA GGACATCCGG 
GTTGTAGGGA ATGAGGCCAA AGGCGAGCAT GTGCCAGGAT TTTGTATTCC TAAGAAGGGA 
GTGACTTCTA TACTTCTGGT GCTGCGTGAA GGTGTTCTAA CTGGTAATCC AGAGCAAAAG 
GAGGAAGCAG CAAAGGCCTT GGGGCTGGTT ATTAAGCTGA CTTCTGCGGA AGCTCTTAAA 
CCATCTGTAG TGAGCATTAC TGGGCCACTT ATTCGTATCT TGGGAGATCG GTTCAGCTGG 
AATGTCAAAG TGGCTCTGCT TGAAACACTA AGCCTTCTTT TAGCCAAGGT TGAGATTGCC 
CTGAAACCAT TTTTGCCTCA ACTGCAAACA ACCTTCACCA AAGCTCTGCA AGATTCAAAC 
CGTGCTGTTC GCCTTAAAGC TGCTGATGCT CTTGGTAAAC TTATTGTTAT CCATGTAAAA 
GTGGATCCTC TTTTTACTGA GCTGCTGAAT GGAATTCGTT CCAGTGATGA CTCTGCCATC 
AGGGACACGA TGCTGCAAGC ACTGCGGTTT GTAACACGAG GAGCTGGTGC AAAAGTAGAT 
GCCACAATTA GGAAGAACAT CAGCACAGTT CTTCTTGGGA TGCTGGGACA CGATGAGGAT 
GCCACCCGCA TGGCTTCAGC TGGCTGCTTA GCAGAACTGT GTGCATTTCT GTCAGAAGAG 
GAGCTCAATA CTGTTCTCCA CCAGCACTTA CTGGACATCT CTGGAATTGA CTGGATGGTG 
AGACATGGAC GAAGCCTGGC CCTCTCAGTG GCTGTGAATG TTGCTCCTTA TAGGCTGTGT 
TCCCCCAAGT ACTATAACAG TGTTCTGGAG ATGATCCTCA GCAATGCCAC TGCTGACAGG 
ATACCCATTG CAGTTAGTGG CATCAGAGGG ATGGGCTTTC TTATGAAATA CCACATGGAA 
GAAGGAGGGA ACCTGCCTCC AAAGCTTTCC AACCTCTTTA TTAAGTGTCT CCAGAACTCA 
TCCAGTGACA TAAAGTTAGT TGCAGAAAAA ATGATCTGGT GGGCAAATAA AAACCATCTT 
CCTGCACTGG ATCCTCAGAC AATTAAACCA ATTCTCAAAG CGCTTCTGGA CAACACAAAA 
GACAAAAACA CCAGTGTAAG AGCTTACAGC GACCAAGCCA TTGTTAATCT TCTGAAGATG 
AGAGTGGGTG AACAAGTGCT TGAGTCTGTT TCCAAGATTT TAGATGCTGC CAGCTTGGAG 
CTTCTCAATG AAAGCTGCAG GAGATCTCTT AAGAAGCTGG CTGGCCAGGC TGACTCTGAT 
GAACAGATAG ATGACACTAT ACTGACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCN1 ( XM_015275398.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015130884.1 Gallus gallus GCN1 eIF2 alpha kinase activator homolog (GCN1), transcript variant X2, mRNA.

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CloneID	OGa42823
Clone ID Related Accession (Same CDS sequence)	XM_015275398.2 , XM_015275398.1
Accession Version	XM_015275398.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	translational activator GCN1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471461.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG ACACGCAGAT ATCTGACACA CTGAAGCGGT TTGCAGTGAA GGTAACTACA 
GCCAGCGTGA AGGAGCGGAG GGAGATCCTC AGTGAGCTGG GAAAATGCAT TTCTGGGAAA 
GATCTTCCCG AGGGTGCAGT GAAAGGCCTT TGCAAACTCT TCTGCCTTAC TCTGCACCGA 
TACCGCGACG CGGCGTCGCG CAGAGCCCTG CAGTTGGCGC TTCAGCAGCT CGCGGAGTCC 
CAGCCAGATG CAACAGCAAA AAACCTTCTG CAGTCACTTC AGTCTTCGGG GATCAGTGGC 
AAGGCTGGAG CTCCCAGTAA GAGCAGTGGA TCTGCAGCCC TGCTGGCACT CTCCTGGACA 
TGCCTGCTGG TACGGACAGT CTTTCCATCA CCTGACAAGA GACAGGGAGA AACCTGGAAA 
AAACTGGTGG AGGTTCAATG TTTACTTCTT TTGGAAGTCT TGGGAGGCTC TCATAAGCAG 
GCAGTGGTTG GAGCAGTGAA GAAACTGAAC CGACTTTGGA AAGAGAACCA AGATCTGGTG 
GATCAGTACC TATCTGTCAT TCTTGGTCTT GAACCAAACC AGAGCTATGC TGCAATGCTG 
GGACTTGTGG TGCAGTTTTG CACAGCTCAG AAAGAGACTG ATATTATTAA ACGCCACAAG 
AGTGCTCTTC TGGATTTCTA TGTGAAGACC ATCCTGATGA GCAAGACAAA GCCTCAGAAG 
CATGTGCTGG ACAGCTGCTC TCCACTTCTT CGATATATCT CTCACGCTGA ATTCAAAGAC 
TTACTGTTGC CAACTCTGCA GAAATCCTTG CTACGGAGCC CTGAGAATGT AATTGAAACA 
ATCTCTTGCT TGCTCTTGTC TGTTAACCTG GATCTTAGCC AGTATGCTCT GGACATTGTG 
AAAGGACTGG CTGGTCATCT GAAATCCAAC AGTGTGCAGC TCATGGATGA AGCAGTTGTC 
GCATTAAAGA ATTTGGCTCG ACAGTGTAGT GATCCTTCAG CCGTGGAATC CCTGAGCAGA 
CACCTTTTTG CCATCTTAGG GGGCTCAGAG GGGAAGCTGA CAGTTGTTGC GCAGAAGATG 
AGCGTCCTTT CAGGGATTGG CAGCCTTAGT CACCATGTGG TTTCTGGATC ATCTAGCCAA 
GCTCTGGCTG GAACCGTGGC TGAGCTTTTC ATCCCTTTTC TGCAGCAAGA AGTCCACGAA 
GGCACGTTGG TTCATGCAGT CTCTGTCCTG GCTCTGTGGT GCGTTCGCTT CACCACTGAA 
GTCCCTAAGA AGATGGTGGA ATGGCTAAAG AAAGCATTTG GCCTTAAAAC CTCTACTTCA 
GCTGTGAGGC ATGCCTACCT GCAGTGCATG CTAGCCTCTT TCAAAGGTGA CACGTTATTA 
CAGGCAATGG ACTTGCTACC GATGCTGATC CAGACAGTAG AAAAAGCAGC ATCTCAAAGT 
ACTCAGATTC CCATGGTAAC AGAAGGAGTT GCTGCAGCTT TGTTGATCTG CAGGATGTCT 
GTAATTGAGG GACAAACTGA GTCTAAGCTG AGTGGCTTCT GGCAGCTGAT CCTAGATGAG 
AAAAAACAAA TTTTTACCTC TGAGAAGTTC TTGCAGTCTG CTTCAGAGGA AGCAATGTGC 
ACTGTGCTGC AGCTGACAGA GCGCCTCCTC TTGGACCATG CACAGCGGCT CCCCGAGAGT 
AAAATCCAGC AGTATCACCG TGCCCTTGTT GCAGTGCTGC TGAGTCGGAC CTGGCACGTT 
CGAAGACAGG CCCATCAGAC AGTTAGGAAG CTTTTGTCCT CTCTGGGAGG GTACAAACTG 
GCCTACGGGC TCTTAGAGGA GCTAAAAGTG GTTCTCAGCT CTCATAAGGT TATGCCTCAA 
GAAGTCCTGG TGATGGAGTC AGGTGAGCTG TCAGAACAAG GGAAGACTTA TATTCCTCCC 
CGTGTCCTTC ATGAGGCACT GTGTGTCATC TCAAGTGTAG CTGGGCTGGA TGTGGATTCC 
TTTGAGACAG AAAAGCTGGC TCTGGAGATG CTGCTGGTGA GCCATCATCC ATCTCTGGTG 
GCAATACAGC CAGGTCTTTG GCCAGCCCTC CTTGTCAAGA TGAAGATAGA TCCCAAAGAC 
TTTATTACTA AACACCTGAA TGACATATTA CCCAGACTCA CTGCCTACTC TCCAGACAAC 
CAGTCATCTA TGAATGCAGT GGGATCTCTC TCTCTGCTCT CACCTGGTAG AGTGCTCCCA 
CAGCTCATCA GCACCATCTC AGCATCAATG CAGAATCCAG CTCTGTGCCA GGTTACTCGA 
GAGGAATTTG CCATCATGAA GACACCGGAG GGAGAACTGT ATGATAAGTC TATAATACAG 
AGTGCTCAAC AGGATAGTAT GAAAAAGGCT AACATGAAGA GGGAGAATAA AGCCTACTCC 
TTCAAGGAAC AAATCATTGA GCTGGAGCTG AAGGAGGAGA TAAAGAAGAA GAAGGGGATA 
AAGGATGAGG TGCAGCTGAC TAGCAAGCAG AAAGAGCTGA TGCATGCACA GCTGGAAAAG 
GAATCTCAGA TAAGAAAACG ACTCAAGGAG CTTGATAATG AACTGGAAAC AGCCTTGGGA 
CTGCTGGACA CTGTTATCAA GAGAAAACCT CCTGGTCTAA CTCAGTACAT CCCTAGTCTT 
GTTGGTTCCT TTTTGCCACT TTTCAGATCG CCTTTGGCTG CTCCAAGAAT TAAGGATCCT 
TTTCTTTCCT TAGCTTCCTG TGTCATGCCT GCTCGACTTA AAACCTTTGG TACTTTAGTG 
AGCCACGTGA CCTTGCGCCT GATGAAACCA GAATGTGAGT TAGATGAATC GTGGTGTCAA 
GAAGAGCTTC CTACTGCTCT CAACAGAGTA GTAAGCTTGC TGCATGCCCA CACCATTCCT 
AGCCGAACTG GCAAAGGAGA GGCAGGTGCT GCCCCATTAT CAGCTCCAGC ATTTTCCCTA 
GTCTTTCCTC TCCTAAAGAC GGCTATGACT GAGACATCCC ATGACAGTGA AGATAAGGAG 
GAATTCCTGG TCAAGATTCT GCAGATCCTT ACAGTTCATG CTCAGCTGAG ATCATCCACA 
AACGGCCAGG CTTTGCTGGT GGATGAGAAT GGCCCGGAGT TGCTGCCCCG ACGGGACATG 
CTGCTTCTGC TGACCCGAGT GATAGCAACA GGCTCTCCAC GGTTACAGGT CTTGGCTTCC 
AATGCACTGA CCTCTGTGTG CACAAGTAGC AGTGGAGAGG ATGGGTGTGC CTATGCAGAA 
CAAGAGGAGA TTGATGTGTT ACTTCAGGCC CTGCAGTCTC CATGCATGAA TGTTCGAGAT 
GCAGCTCTCA GGGGACTGAT GGAGCTACAG ATGGTTCTAC CAACCCCAGA CAGCGATGAA 
AAGAATGGAT TAAATCTGTT GCGTCGCCTT TGGGTAGTAA AGTTTGATGT GGAAGATGAG 
ATTCAGAAGC TGGCAGAGAG GTTGTGGGAG TCCATGGGTC TGGAACTACA GCCTGACCTC 
TGTTCTCTGC TGATCAAGGA TGTCATCTAC CATGAAGAGG CAGTGCGACA AGCAGGTGCC 
GAAGCTCTTT CCAAAGCTGT AGCTCAGTAT CAGCACCAGG CAGCAGAGGT TATGAATAAA 
CTTACAGAGA TTTACCAGGA GAAGCTCTAT AGGCCACCTC CAGTTTTGGA TGCTTTGGGA 
CGAGTGATAT CTGAATCACC ACCTGACCAG TGGGAAGCAA GGTGTGGCAT TGCTTTGGCC 
CTTAACAAGC TCTCAGAGCA TCTGGACAGT TCTCAGGTGA AGCCACTCTT TCAGTTTTTT 
GTACCAGATG CTCTGAATGA TCGCAATCCT GAAGTCAGGA AGTGCATGTT AGATGCAGCT 
CTTTCAACGC TCAACACTCA TGGCAAAGAC AATGTTAACT CTCTCTTGCC AGTGTTCGAA 
GAGTTCCTGA AAAATGCTCC TAATGATGCC AGTTATGATG CTGTCAGGCA GAGTGTGGTC 
ATTCTGATGG GCTCACTGGC AAAACATCTG GACAAAAGTG ACCCTAAAGT GAAACCAATT 
GTTGGCAAAC TGATTGCAGC CTTGTCCACA CCATCTCAGC AGGTTCAAGA GTCAGTGGCA 
AGCTGTCTTC CACCACTGGT GCCTGCAATT AAGGAGGATG CAGGAGGAAT GATACAGAAG 
CTAATGCAGC TGCTTTTAGA ATCTGATAAA TATGCCGAGC GCAAAGGTGC AGCTTATGGA 
CTGGCAGGTC TGGTGAAAGG CCTTGGCATC CTTTCTCTCA AGCAGCAGGA GATGATGACC 
ACACTGACTG ATGCTATCCA AGACAAGAAG AACTTTCGTC GGCGGGAGGG GGCTCTATTT 
GCCTTTGAGA TGCTCTGCAG CATGCTTGGA AAGCTCTTTG AGCCCTATGT GGTTCATGTG 
CTACCTCACT TGTTGCTTTG TTTTGGAGAT GGGAACCAGT ATGTGCGAGA GGCTGCAGAT 
GACTGTGCCA AAGCAGTGAT GAGCAACTTG AGTACTCATG GAGTGAAGCT GGTTTTGCCA 
TCACTTCTTG CTGCACTAGA AGAGGAATCT TGGAGAACCA AAGCTGGGTC AGTGGAACTG 
CTGGGAGCTA TGGCATACTG TGCTCCCAAA CAGCTCTCCT CCTGCTTACC CAATATTGTG 
CCAAAACTTA CTGAAGTGCT CACAGATTCT CATGTGAAGG TGCAGAATGC AGGTCAGCAA 
GCTCTCAGGC AGATTGGGTC CGTCATCAGA AATCCAGAGA TCCTTGCAAT TGCTCCAGTT 
TTGTTGGATG CTCTCACTGA CCCCTCCAGG AAGACCCAAA AGTGTCTGCA GACTCTACTG 
GATACCAAAT TTGTCCACTT CATTGATGCT CCATCCTTAG CACTTATTAT GCCAATTGTT 
CAGAGAGCTT TTCAGGATCG TTCCACAGAT ACCAGGAAAA TGGCTGCCCA GATTATTGGG 
AACATGTACT CCTTGACTGA TCAGAAGGAT CTGGCTCCTT ACCTACCCAG TGTGACTCCT 
GGACTGAAGG CATCCCTGCT GGATCCTGTA CCTGAAGTGC GTACAGTATC TGCAAAGGCA 
CTGGGAGCCA TGGTGAAGGG CATGGGAGAA TCATGCTTTG AGGATTTGTT GCCATGGCTG 
ATGGAGACAC TGACATACGA GCAAAGCTCA GTGGACCGAT CTGGTGCAGC TCAAGGTCTG 
GCTGAAGTTA TGGCTGGTTT AGGGGTGGAG AAGCTGGAGA AACTTATGCC AGAGATTGTA 
GCTACTGCCA GTAAGGTGGA TATTGCTCCA CATGTACGAG ATGGTTATAT AATGATGTTT 
AACTACCTGC CAATTACTTT TGGAGACAAG TTCATTCCGT ATGTTGGTCC CATCATCCCT 
TGTATCCTTA AGGCACTGGC AGATGAGAAT GAGTTTGTGC GGGACACTGC CCTGCGTGCT 
GGCCAGAGGA TCATAAGTAT GTATGCAGAG ACAGCCATTG CACTGCTTCT GCCACAGCTT 
GAGGATGGCC TGTTTGATGA CCTGTGGAGG ATAAGGTTTA GCTCAGTTCA GCTCCTTGGA 
GATCTTTTAT TCCATATCTC AGGAGTCACT GGAAAAATGA CCACAGAAAC TGCCTCAGAA 
GATGACAACT TTGGAACAGC CCAGTCAAAC AAGGCTATTA TTAATGCTCT TGGGGTGGAG 
AGGAGGAACA GGGTGCTGGC AGGGCTGTAC ATGGGCCGCT CTGACACCCA GCTGGTAGTG 
CGTCAAGCTT CGTTACACGT TTGGAAGATT GTAGTTTCAA ACACTCCTCG CACGCTGCGT 
GAAATTTTGC CAACACTCTT TGGGCTTCTG CTGAAATTCT TGGCCAGTAC TTGTGCAGAT 
AAGCGAACGG TTGCAGCACG GACATTAGGA GACCTTGTCC GAAAGTTAGG AGAGAAGATC 
CTTCCTGAAA TCATTCCCAT TCTGGAAGAT GGCCTAAGGT CAGATAAGAA TGATGAAAGG 
CAAGGAGTCT GCATTGGGCT GAGTGAGATA ATGAAGTCTA CCAGTAGAGA TGCGGTACTG 
GTTTTCTCTG AGTCCCTAGT GCCTACAGTG AGAAAAGCAT TGTGTGACCC TCTGGAAGAA 
GTGAGAGAGG CTGCAGCTAA AACATTTGAA CAGTTGCACT CAACAATTGG GCATCAGGCT 
CTTGAAGATA TCCTTCCGTT TTTGTTGAAG CAGCTGGATA ATGAAGAAAC ATCTGACTTT 
GCTGTGGATG GTCTTAAACA AGTTATGGCT GTCAAGAGCC GTGTGGTACT GCCTTATTTG 
GTGCCAAAGC TGACTACTCC CCCTGTGAAC ACCCGTGTGC TAGCTTTCCT CTCATCAGTG 
GCAGGGGATG CTCTGACAAG GCACTTGAGT GTCATCCTGC CTGCTATGAT GTCTGCACTG 
AAAGAGAAGC TGGGGACTAG TGAAGAGCAA CTGGAAATGG CAAATTGCCA GTCTGTAATC 
CTTTCTGTGG AAGATGATGT TGGACAAAGA ATTATAACTG AGGATTTACT AGAAGCTACC 
CGCAGCCCTG ACGTGGGAAT GAGACAAGCA GCAGCTATCA TTCTGAATAT CTACTGCTCC 
AAGACAAAAG CAGACTACAC TGGTCATCTC AAGAACTTGG TTTCAGGCCT GATACGTTTA 
TTTAATGATA CCAATCCTGT AGTTTTGAAT GAAAGCTGGG ATGCACTTAA TTCCATCACC 
AAGAAATTAG ATGCTGGGAA CCAGCTTGCT CTCATTGAGG ACCTACACAA GGACATCCGG 
GTTGTAGGGA ATGAGGCCAA AGGCGAGCAT GTGCCAGGAT TTTGTATTCC TAAGAAGGGA 
GTGACTTCTA TACTTCTGGT GCTGCGTGAA GGTGTTCTAA CTGGTAATCC AGAGCAAAAG 
GAGGAAGCAG CAAAGGCCTT GGGGCTGGTT ATTAAGCTGA CTTCTGCGGA AGCTCTTAAA 
CCATCTGTAG TGAGCATTAC TGGGCCACTT ATTCGTATCT TGGGAGATCG GTTCAGCTGG 
AATGTCAAAG TGGCTCTGCT TGAAACACTA AGCCTTCTTT TAGCCAAGGT TGAGATTGCC 
CTGAAACCAT TTTTGCCTCA ACTGCAAACA ACCTTCACCA AAGCTCTGCA AGATTCAAAC 
CGTGCTGTTC GCCTTAAAGC TGCTGATGCT CTTGGTAAAC TTATTGTTAT CCATGTAAAA 
GTGGATCCTC TTTTTACTGA GCTGCTGAAT GGAATTCGTT CCAGTGATGA CTCTGCCATC 
AGGGACACGA TGCTGCAAGC ACTGCGGTTT GTAACACGAG GAGCTGGTGC AAAAGTAGAT 
GCCACAATTA GGAAGAACAT CAGCACAGTT CTTCTTGGGA TGCTGGGACA CGATGAGGAT 
GCCACCCGCA TGGCTTCAGC TGGCTGCTTA GCAGAACTGT GTGCATTTCT GTCAGAAGAG 
GAGCTCAATA CTGTTCTCCA CCAGCACTTA CTGGACATCT CTGGAATTGA CTGGATGGTG 
AGACATGGAC GAAGCCTGGC CCTCTCAGTG GCTGTGAATG TTGCTCCTTA TAGGCTGTGT 
TCCCCCAAGT ACTATAACAG TGTTCTGGAG ATGATCCTCA GCAATGCCAC TGCTGACAGG 
ATACCCATTG CAGTTAGTGG CATCAGAGGG ATGGGCTTTC TTATGAAATA CCACATGGAA 
GAAGGAGGGA ACCTGCCTCC AAAGCTTTCC AACCTCTTTA TTAAGTGTCT CCAGAACTCA 
TCCAGTGACA TAAAGTTAGT TGCAGAAAAA ATGATCTGGT GGGCAAATAA AAACCATCTT 
CCTGCACTGG ATCCTCAGAC AATTAAACCA ATTCTCAAAG CGCTTCTGGA CAACACAAAA 
GACAAAAACA CCAGTGTAAG AGCTTACAGC GACCAAGCCA TTGTTAATCT TCTGAAGATG 
AGAGTGGGTG AACAAGTGCT TGAGTCTGTT TCCAAGATTT TAGATGCTGC CAGCTTGGAG 
CTTCTCAATG AAAGCTGCAG GAGATCTCTT AAGAAGCTGG CTGGCCAGGC TGACTCTGAT 
GAACAGATAG ATGACACTAT ACTGACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015130884.1
CDS	63..8072
Translation	MAADTQISDTLKRFAVKVTTASVKERREILSELGKCISGKDLPEGAVKGLCKLFCLTLHRYRDAASRRALQLALQQLAESQPDATAKNLLQSLQSSGISGKAGAPSKSSGSAALLALSWTCLLVRTVFPSPDKRQGETWKKLVEVQCLLLLEVLGGSHKQAVVGAVKKLNRLWKENQDLVDQYLSVILGLEPNQSYAAMLGLVVQFCTAQKETDIIKRHKSALLDFYVKTILMSKTKPQKHVLDSCSPLLRYISHAEFKDLLLPTLQKSLLRSPENVIETISCLLLSVNLDLSQYALDIVKGLAGHLKSNSVQLMDEAVVALKNLARQCSDPSAVESLSRHLFAILGGSEGKLTVVAQKMSVLSGIGSLSHHVVSGSSSQALAGTVAELFIPFLQQEVHEGTLVHAVSVLALWCVRFTTEVPKKMVEWLKKAFGLKTSTSAVRHAYLQCMLASFKGDTLLQAMDLLPMLIQTVEKAASQSTQIPMVTEGVAAALLICRMSVIEGQTESKLSGFWQLILDEKKQIFTSEKFLQSASEEAMCTVLQLTERLLLDHAQRLPESKIQQYHRALVAVLLSRTWHVRRQAHQTVRKLLSSLGGYKLAYGLLEELKVVLSSHKVMPQEVLVMESGELSEQGKTYIPPRVLHEALCVISSVAGLDVDSFETEKLALEMLLVSHHPSLVAIQPGLWPALLVKMKIDPKDFITKHLNDILPRLTAYSPDNQSSMNAVGSLSLLSPGRVLPQLISTISASMQNPALCQVTREEFAIMKTPEGELYDKSIIQSAQQDSMKKANMKRENKAYSFKEQIIELELKEEIKKKKGIKDEVQLTSKQKELMHAQLEKESQIRKRLKELDNELETALGLLDTVIKRKPPGLTQYIPSLVGSFLPLFRSPLAAPRIKDPFLSLASCVMPARLKTFGTLVSHVTLRLMKPECELDESWCQEELPTALNRVVSLLHAHTIPSRTGKGEAGAAPLSAPAFSLVFPLLKTAMTETSHDSEDKEEFLVKILQILTVHAQLRSSTNGQALLVDENGPELLPRRDMLLLLTRVIATGSPRLQVLASNALTSVCTSSSGEDGCAYAEQEEIDVLLQALQSPCMNVRDAALRGLMELQMVLPTPDSDEKNGLNLLRRLWVVKFDVEDEIQKLAERLWESMGLELQPDLCSLLIKDVIYHEEAVRQAGAEALSKAVAQYQHQAAEVMNKLTEIYQEKLYRPPPVLDALGRVISESPPDQWEARCGIALALNKLSEHLDSSQVKPLFQFFVPDALNDRNPEVRKCMLDAALSTLNTHGKDNVNSLLPVFEEFLKNAPNDASYDAVRQSVVILMGSLAKHLDKSDPKVKPIVGKLIAALSTPSQQVQESVASCLPPLVPAIKEDAGGMIQKLMQLLLESDKYAERKGAAYGLAGLVKGLGILSLKQQEMMTTLTDAIQDKKNFRRREGALFAFEMLCSMLGKLFEPYVVHVLPHLLLCFGDGNQYVREAADDCAKAVMSNLSTHGVKLVLPSLLAALEEESWRTKAGSVELLGAMAYCAPKQLSSCLPNIVPKLTEVLTDSHVKVQNAGQQALRQIGSVIRNPEILAIAPVLLDALTDPSRKTQKCLQTLLDTKFVHFIDAPSLALIMPIVQRAFQDRSTDTRKMAAQIIGNMYSLTDQKDLAPYLPSVTPGLKASLLDPVPEVRTVSAKALGAMVKGMGESCFEDLLPWLMETLTYEQSSVDRSGAAQGLAEVMAGLGVEKLEKLMPEIVATASKVDIAPHVRDGYIMMFNYLPITFGDKFIPYVGPIIPCILKALADENEFVRDTALRAGQRIISMYAETAIALLLPQLEDGLFDDLWRIRFSSVQLLGDLLFHISGVTGKMTTETASEDDNFGTAQSNKAIINALGVERRNRVLAGLYMGRSDTQLVVRQASLHVWKIVVSNTPRTLREILPTLFGLLLKFLASTCADKRTVAARTLGDLVRKLGEKILPEIIPILEDGLRSDKNDERQGVCIGLSEIMKSTSRDAVLVFSESLVPTVRKALCDPLEEVREAAAKTFEQLHSTIGHQALEDILPFLLKQLDNEETSDFAVDGLKQVMAVKSRVVLPYLVPKLTTPPVNTRVLAFLSSVAGDALTRHLSVILPAMMSALKEKLGTSEEQLEMANCQSVILSVEDDVGQRIITEDLLEATRSPDVGMRQAAAIILNIYCSKTKADYTGHLKNLVSGLIRLFNDTNPVVLNESWDALNSITKKLDAGNQLALIEDLHKDIRVVGNEAKGEHVPGFCIPKKGVTSILLVLREGVLTGNPEQKEEAAKALGLVIKLTSAEALKPSVVSITGPLIRILGDRFSWNVKVALLETLSLLLAKVEIALKPFLPQLQTTFTKALQDSNRAVRLKAADALGKLIVIHVKVDPLFTELLNGIRSSDDSAIRDTMLQALRFVTRGAGAKVDATIRKNISTVLLGMLGHDEDATRMASAGCLAELCAFLSEEELNTVLHQHLLDISGIDWMVRHGRSLALSVAVNVAPYRLCSPKYYNSVLEMILSNATADRIPIAVSGIRGMGFLMKYHMEEGGNLPPKLSNLFIKCLQNSSSDIKLVAEKMIWWANKNHLPALDPQTIKPILKALLDNTKDKNTSVRAYSDQAIVNLLKMRVGEQVLESVSKILDAASLELLNESCRRSLKKLAGQADSDEQIDDTILT

Target ORF information:

RefSeq Version	XM_015275398.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus GCN1 eIF2 alpha kinase activator homolog (GCN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015275398.1

ORF Insert Sequence:

ATGGCGGCCG ACACGCAGAT ATCTGACACA CTGAAGCGGT TTGCAGTGAA GGTAACTACA 
GCCAGCGTGA AGGAGCGGAG GGAGATCCTC AGTGAGCTGG GAAAATGCAT TTCTGGGAAA 
GATCTTCCCG AGGGTGCAGT GAAAGGCCTT TGCAAACTCT TCTGCCTTAC TCTGCACCGA 
TACCGCGACG CGGCGTCGCG CAGAGCCCTG CAGTTGGCGC TTCAGCAGCT CGCGGAGTCC 
CAGCCAGATG CAACAGCAAA AAACCTTCTG CAGTCACTTC AGTCTTCGGG GATCAGTGGC 
AAGGCTGGAG CTCCCAGTAA GAGCAGTGGA TCTGCAGCCC TGCTGGCACT CTCCTGGACA 
TGCCTGCTGG TACGGACAGT CTTTCCATCA CCTGACAAGA GACAGGGAGA AACCTGGAAA 
AAACTGGTGG AGGTTCAATG TTTACTTCTT TTGGAAGTCT TGGGAGGCTC TCATAAGCAG 
GCAGTGGTTG GAGCAGTGAA GAAACTGAAC CGACTTTGGA AAGAGAACCA AGATCTGGTG 
GATCAGTACC TATCTGTCAT TCTTGGTCTT GAACCAAACC AGAGCTATGC TGCAATGCTG 
GGACTTGTGG TGCAGTTTTG CACAGCTCAG AAAGAGACTG ATATTATTAA ACGCCACAAG 
AGTGCTCTTC TGGATTTCTA TGTGAAGACC ATCCTGATGA GCAAGACAAA GCCTCAGAAG 
CATGTGCTGG ACAGCTGCTC TCCACTTCTT CGATATATCT CTCACGCTGA ATTCAAAGAC 
TTACTGTTGC CAACTCTGCA GAAATCCTTG CTACGGAGCC CTGAGAATGT AATTGAAACA 
ATCTCTTGCT TGCTCTTGTC TGTTAACCTG GATCTTAGCC AGTATGCTCT GGACATTGTG 
AAAGGACTGG CTGGTCATCT GAAATCCAAC AGTGTGCAGC TCATGGATGA AGCAGTTGTC 
GCATTAAAGA ATTTGGCTCG ACAGTGTAGT GATCCTTCAG CCGTGGAATC CCTGAGCAGA 
CACCTTTTTG CCATCTTAGG GGGCTCAGAG GGGAAGCTGA CAGTTGTTGC GCAGAAGATG 
AGCGTCCTTT CAGGGATTGG CAGCCTTAGT CACCATGTGG TTTCTGGATC ATCTAGCCAA 
GCTCTGGCTG GAACCGTGGC TGAGCTTTTC ATCCCTTTTC TGCAGCAAGA AGTCCACGAA 
GGCACGTTGG TTCATGCAGT CTCTGTCCTG GCTCTGTGGT GCGTTCGCTT CACCACTGAA 
GTCCCTAAGA AGATGGTGGA ATGGCTAAAG AAAGCATTTG GCCTTAAAAC CTCTACTTCA 
GCTGTGAGGC ATGCCTACCT GCAGTGCATG CTAGCCTCTT TCAAAGGTGA CACGTTATTA 
CAGGCAATGG ACTTGCTACC GATGCTGATC CAGACAGTAG AAAAAGCAGC ATCTCAAAGT 
ACTCAGATTC CCATGGTAAC AGAAGGAGTT GCTGCAGCTT TGTTGATCTG CAGGATGTCT 
GTAATTGAGG GACAAACTGA GTCTAAGCTG AGTGGCTTCT GGCAGCTGAT CCTAGATGAG 
AAAAAACAAA TTTTTACCTC TGAGAAGTTC TTGCAGTCTG CTTCAGAGGA AGCAATGTGC 
ACTGTGCTGC AGCTGACAGA GCGCCTCCTC TTGGACCATG CACAGCGGCT CCCCGAGAGT 
AAAATCCAGC AGTATCACCG TGCCCTTGTT GCAGTGCTGC TGAGTCGGAC CTGGCACGTT 
CGAAGACAGG CCCATCAGAC AGTTAGGAAG CTTTTGTCCT CTCTGGGAGG GTACAAACTG 
GCCTACGGGC TCTTAGAGGA GCTAAAAGTG GTTCTCAGCT CTCATAAGGT TATGCCTCAA 
GAAGTCCTGG TGATGGAGTC AGGTGAGCTG TCAGAACAAG GGAAGACTTA TATTCCTCCC 
CGTGTCCTTC ATGAGGCACT GTGTGTCATC TCAAGTGTAG CTGGGCTGGA TGTGGATTCC 
TTTGAGACAG AAAAGCTGGC TCTGGAGATG CTGCTGGTGA GCCATCATCC ATCTCTGGTG 
GCAATACAGC CAGGTCTTTG GCCAGCCCTC CTTGTCAAGA TGAAGATAGA TCCCAAAGAC 
TTTATTACTA AACACCTGAA TGACATATTA CCCAGACTCA CTGCCTACTC TCCAGACAAC 
CAGTCATCTA TGAATGCAGT GGGATCTCTC TCTCTGCTCT CACCTGGTAG AGTGCTCCCA 
CAGCTCATCA GCACCATCTC AGCATCAATG CAGAATCCAG CTCTGTGCCA GGTTACTCGA 
GAGGAATTTG CCATCATGAA GACACCGGAG GGAGAACTGT ATGATAAGTC TATAATACAG 
AGTGCTCAAC AGGATAGTAT GAAAAAGGCT AACATGAAGA GGGAGAATAA AGCCTACTCC 
TTCAAGGAAC AAATCATTGA GCTGGAGCTG AAGGAGGAGA TAAAGAAGAA GAAGGGGATA 
AAGGATGAGG TGCAGCTGAC TAGCAAGCAG AAAGAGCTGA TGCATGCACA GCTGGAAAAG 
GAATCTCAGA TAAGAAAACG ACTCAAGGAG CTTGATAATG AACTGGAAAC AGCCTTGGGA 
CTGCTGGACA CTGTTATCAA GAGAAAACCT CCTGGTCTAA CTCAGTACAT CCCTAGTCTT 
GTTGGTTCCT TTTTGCCACT TTTCAGATCG CCTTTGGCTG CTCCAAGAAT TAAGGATCCT 
TTTCTTTCCT TAGCTTCCTG TGTCATGCCT GCTCGACTTA AAACCTTTGG TACTTTAGTG 
AGCCACGTGA CCTTGCGCCT GATGAAACCA GAATGTGAGT TAGATGAATC GTGGTGTCAA 
GAAGAGCTTC CTACTGCTCT CAACAGAGTA GTAAGCTTGC TGCATGCCCA CACCATTCCT 
AGCCGAACTG GCAAAGGAGA GGCAGGTGCT GCCCCATTAT CAGCTCCAGC ATTTTCCCTA 
GTCTTTCCTC TCCTAAAGAC GGCTATGACT GAGACATCCC ATGACAGTGA AGATAAGGAG 
GAATTCCTGG TCAAGATTCT GCAGATCCTT ACAGTTCATG CTCAGCTGAG ATCATCCACA 
AACGGCCAGG CTTTGCTGGT GGATGAGAAT GGCCCGGAGT TGCTGCCCCG ACGGGACATG 
CTGCTTCTGC TGACCCGAGT GATAGCAACA GGCTCTCCAC GGTTACAGGT CTTGGCTTCC 
AATGCACTGA CCTCTGTGTG CACAAGTAGC AGTGGAGAGG ATGGGTGTGC CTATGCAGAA 
CAAGAGGAGA TTGATGTGTT ACTTCAGGCC CTGCAGTCTC CATGCATGAA TGTTCGAGAT 
GCAGCTCTCA GGGGACTGAT GGAGCTACAG ATGGTTCTAC CAACCCCAGA CAGCGATGAA 
AAGAATGGAT TAAATCTGTT GCGTCGCCTT TGGGTAGTAA AGTTTGATGT GGAAGATGAG 
ATTCAGAAGC TGGCAGAGAG GTTGTGGGAG TCCATGGGTC TGGAACTACA GCCTGACCTC 
TGTTCTCTGC TGATCAAGGA TGTCATCTAC CATGAAGAGG CAGTGCGACA AGCAGGTGCC 
GAAGCTCTTT CCAAAGCTGT AGCTCAGTAT CAGCACCAGG CAGCAGAGGT TATGAATAAA 
CTTACAGAGA TTTACCAGGA GAAGCTCTAT AGGCCACCTC CAGTTTTGGA TGCTTTGGGA 
CGAGTGATAT CTGAATCACC ACCTGACCAG TGGGAAGCAA GGTGTGGCAT TGCTTTGGCC 
CTTAACAAGC TCTCAGAGCA TCTGGACAGT TCTCAGGTGA AGCCACTCTT TCAGTTTTTT 
GTACCAGATG CTCTGAATGA TCGCAATCCT GAAGTCAGGA AGTGCATGTT AGATGCAGCT 
CTTTCAACGC TCAACACTCA TGGCAAAGAC AATGTTAACT CTCTCTTGCC AGTGTTCGAA 
GAGTTCCTGA AAAATGCTCC TAATGATGCC AGTTATGATG CTGTCAGGCA GAGTGTGGTC 
ATTCTGATGG GCTCACTGGC AAAACATCTG GACAAAAGTG ACCCTAAAGT GAAACCAATT 
GTTGGCAAAC TGATTGCAGC CTTGTCCACA CCATCTCAGC AGGTTCAAGA GTCAGTGGCA 
AGCTGTCTTC CACCACTGGT GCCTGCAATT AAGGAGGATG CAGGAGGAAT GATACAGAAG 
CTAATGCAGC TGCTTTTAGA ATCTGATAAA TATGCCGAGC GCAAAGGTGC AGCTTATGGA 
CTGGCAGGTC TGGTGAAAGG CCTTGGCATC CTTTCTCTCA AGCAGCAGGA GATGATGACC 
ACACTGACTG ATGCTATCCA AGACAAGAAG AACTTTCGTC GGCGGGAGGG GGCTCTATTT 
GCCTTTGAGA TGCTCTGCAG CATGCTTGGA AAGCTCTTTG AGCCCTATGT GGTTCATGTG 
CTACCTCACT TGTTGCTTTG TTTTGGAGAT GGGAACCAGT ATGTGCGAGA GGCTGCAGAT 
GACTGTGCCA AAGCAGTGAT GAGCAACTTG AGTACTCATG GAGTGAAGCT GGTTTTGCCA 
TCACTTCTTG CTGCACTAGA AGAGGAATCT TGGAGAACCA AAGCTGGGTC AGTGGAACTG 
CTGGGAGCTA TGGCATACTG TGCTCCCAAA CAGCTCTCCT CCTGCTTACC CAATATTGTG 
CCAAAACTTA CTGAAGTGCT CACAGATTCT CATGTGAAGG TGCAGAATGC AGGTCAGCAA 
GCTCTCAGGC AGATTGGGTC CGTCATCAGA AATCCAGAGA TCCTTGCAAT TGCTCCAGTT 
TTGTTGGATG CTCTCACTGA CCCCTCCAGG AAGACCCAAA AGTGTCTGCA GACTCTACTG 
GATACCAAAT TTGTCCACTT CATTGATGCT CCATCCTTAG CACTTATTAT GCCAATTGTT 
CAGAGAGCTT TTCAGGATCG TTCCACAGAT ACCAGGAAAA TGGCTGCCCA GATTATTGGG 
AACATGTACT CCTTGACTGA TCAGAAGGAT CTGGCTCCTT ACCTACCCAG TGTGACTCCT 
GGACTGAAGG CATCCCTGCT GGATCCTGTA CCTGAAGTGC GTACAGTATC TGCAAAGGCA 
CTGGGAGCCA TGGTGAAGGG CATGGGAGAA TCATGCTTTG AGGATTTGTT GCCATGGCTG 
ATGGAGACAC TGACATACGA GCAAAGCTCA GTGGACCGAT CTGGTGCAGC TCAAGGTCTG 
GCTGAAGTTA TGGCTGGTTT AGGGGTGGAG AAGCTGGAGA AACTTATGCC AGAGATTGTA 
GCTACTGCCA GTAAGGTGGA TATTGCTCCA CATGTACGAG ATGGTTATAT AATGATGTTT 
AACTACCTGC CAATTACTTT TGGAGACAAG TTCATTCCGT ATGTTGGTCC CATCATCCCT 
TGTATCCTTA AGGCACTGGC AGATGAGAAT GAGTTTGTGC GGGACACTGC CCTGCGTGCT 
GGCCAGAGGA TCATAAGTAT GTATGCAGAG ACAGCCATTG CACTGCTTCT GCCACAGCTT 
GAGGATGGCC TGTTTGATGA CCTGTGGAGG ATAAGGTTTA GCTCAGTTCA GCTCCTTGGA 
GATCTTTTAT TCCATATCTC AGGAGTCACT GGAAAAATGA CCACAGAAAC TGCCTCAGAA 
GATGACAACT TTGGAACAGC CCAGTCAAAC AAGGCTATTA TTAATGCTCT TGGGGTGGAG 
AGGAGGAACA GGGTGCTGGC AGGGCTGTAC ATGGGCCGCT CTGACACCCA GCTGGTAGTG 
CGTCAAGCTT CGTTACACGT TTGGAAGATT GTAGTTTCAA ACACTCCTCG CACGCTGCGT 
GAAATTTTGC CAACACTCTT TGGGCTTCTG CTGAAATTCT TGGCCAGTAC TTGTGCAGAT 
AAGCGAACGG TTGCAGCACG GACATTAGGA GACCTTGTCC GAAAGTTAGG AGAGAAGATC 
CTTCCTGAAA TCATTCCCAT TCTGGAAGAT GGCCTAAGGT CAGATAAGAA TGATGAAAGG 
CAAGGAGTCT GCATTGGGCT GAGTGAGATA ATGAAGTCTA CCAGTAGAGA TGCGGTACTG 
GTTTTCTCTG AGTCCCTAGT GCCTACAGTG AGAAAAGCAT TGTGTGACCC TCTGGAAGAA 
GTGAGAGAGG CTGCAGCTAA AACATTTGAA CAGTTGCACT CAACAATTGG GCATCAGGCT 
CTTGAAGATA TCCTTCCGTT TTTGTTGAAG CAGCTGGATA ATGAAGAAAC ATCTGACTTT 
GCTGTGGATG GTCTTAAACA AGTTATGGCT GTCAAGAGCC GTGTGGTACT GCCTTATTTG 
GTGCCAAAGC TGACTACTCC CCCTGTGAAC ACCCGTGTGC TAGCTTTCCT CTCATCAGTG 
GCAGGGGATG CTCTGACAAG GCACTTGAGT GTCATCCTGC CTGCTATGAT GTCTGCACTG 
AAAGAGAAGC TGGGGACTAG TGAAGAGCAA CTGGAAATGG CAAATTGCCA GTCTGTAATC 
CTTTCTGTGG AAGATGATGT TGGACAAAGA ATTATAACTG AGGATTTACT AGAAGCTACC 
CGCAGCCCTG ACGTGGGAAT GAGACAAGCA GCAGCTATCA TTCTGAATAT CTACTGCTCC 
AAGACAAAAG CAGACTACAC TGGTCATCTC AAGAACTTGG TTTCAGGCCT GATACGTTTA 
TTTAATGATA CCAATCCTGT AGTTTTGAAT GAAAGCTGGG ATGCACTTAA TTCCATCACC 
AAGAAATTAG ATGCTGGGAA CCAGCTTGCT CTCATTGAGG ACCTACACAA GGACATCCGG 
GTTGTAGGGA ATGAGGCCAA AGGCGAGCAT GTGCCAGGAT TTTGTATTCC TAAGAAGGGA 
GTGACTTCTA TACTTCTGGT GCTGCGTGAA GGTGTTCTAA CTGGTAATCC AGAGCAAAAG 
GAGGAAGCAG CAAAGGCCTT GGGGCTGGTT ATTAAGCTGA CTTCTGCGGA AGCTCTTAAA 
CCATCTGTAG TGAGCATTAC TGGGCCACTT ATTCGTATCT TGGGAGATCG GTTCAGCTGG 
AATGTCAAAG TGGCTCTGCT TGAAACACTA AGCCTTCTTT TAGCCAAGGT TGAGATTGCC 
CTGAAACCAT TTTTGCCTCA ACTGCAAACA ACCTTCACCA AAGCTCTGCA AGATTCAAAC 
CGTGCTGTTC GCCTTAAAGC TGCTGATGCT CTTGGTAAAC TTATTGTTAT CCATGTAAAA 
GTGGATCCTC TTTTTACTGA GCTGCTGAAT GGAATTCGTT CCAGTGATGA CTCTGCCATC 
AGGGACACGA TGCTGCAAGC ACTGCGGTTT GTAACACGAG GAGCTGGTGC AAAAGTAGAT 
GCCACAATTA GGAAGAACAT CAGCACAGTT CTTCTTGGGA TGCTGGGACA CGATGAGGAT 
GCCACCCGCA TGGCTTCAGC TGGCTGCTTA GCAGAACTGT GTGCATTTCT GTCAGAAGAG 
GAGCTCAATA CTGTTCTCCA CCAGCACTTA CTGGACATCT CTGGAATTGA CTGGATGGTG 
AGACATGGAC GAAGCCTGGC CCTCTCAGTG GCTGTGAATG TTGCTCCTTA TAGGCTGTGT 
TCCCCCAAGT ACTATAACAG TGTTCTGGAG ATGATCCTCA GCAATGCCAC TGCTGACAGG 
ATACCCATTG CAGTTAGTGG CATCAGAGGG ATGGGCTTTC TTATGAAATA CCACATGGAA 
GAAGGAGGGA ACCTGCCTCC AAAGCTTTCC AACCTCTTTA TTAAGTGTCT CCAGAACTCA 
TCCAGTGACA TAAAGTTAGT TGCAGAAAAA ATGATCTGGT GGGCAAATAA AAACCATCTT 
CCTGCACTGG ATCCTCAGAC AATTAAACCA ATTCTCAAAG CGCTTCTGGA CAACACAAAA 
GACAAAAACA CCAGTGTAAG AGCTTACAGC GACCAAGCCA TTGTTAATCT TCTGAAGATG 
AGAGTGGGTG AACAAGTGCT TGAGTCTGTT TCCAAGATTT TAGATGCTGC CAGCTTGGAG 
CTTCTCAATG AAAGCTGCAG GAGATCTCTT AAGAAGCTGG CTGGCCAGGC TGACTCTGAT 
GAACAGATAG ATGACACTAT ACTGACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

GCN1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報