GCN1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	GCN1
Entrez Gene ID	852680
Full Name	Gcn1p
Synonyms	AAS103,NDR1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181060.3	NP_011320.3	Gcn1p

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GCN1L1	XP_534701.2
Danio rerio (zebrafish)	gcn1l1	XP_002667084.2
Caenorhabditis elegans (roundworm)	gcn-1	NP_497506.3
Oryza sativa (rice)	Os03g0721200	NP_001051102.1
Saccharomyces cerevisiae (baker's yeast)	GCN1	NP_011320.3
Schizosaccharomyces pombe (fission yeast)	SPAC18G6.05c	NP_593669.1
Magnaporthe oryzae (rice blast fungus)	MGG_04710	XP_003710832.1
Arabidopsis thaliana (thale cress)	ILA	NP_176659.6
Pan troglodytes (chimpanzee)	GCN1L1	XP_001159608.2
Mus musculus (house mouse)	Gcn1l1	NP_766307.2
Rattus norvegicus (Norway rat)	Gcn1l1	NP_001162135.1
Eremothecium gossypii	AGOS_AFR169W	NP_985716.2
Homo sapiens (human)	GCN1L1	NP_006827.1
Bos taurus (cattle)	GCN1L1	XP_002694568.1
Gallus gallus (chicken)	GCN1L1	XP_003642271.2
Drosophila melanogaster (fruit fly)	CG17514	NP_001015316.1
Neurospora crassa	NCU05803	XP_960063.1
Xenopus tropicalis (tropical clawed frog)	gcn1l1	NP_001096529.1
	GCN1L1	XP_001087027.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001700	XP_321391.4
Kluyveromyces lactis	KLLA0F12430g	XP_455642.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Gcn1 contacts ribosome-bound Rps10 to efficiently mediate Gcn2 activation.
Title: Gcn1 contacts the small ribosomal protein Rps10, which is required for full activation of the protein kinase Gcn2.

Quantitative trait loci analysis suggest that polymorphism in the coding region of GCN1, MDS3, ARG81 and BIO3 could account for differences in nitrogen requirement.
Title: A genetic approach of wine yeast fermentation capacity in nitrogen-starvation reveals the key role of nitrogen signaling.

Yih1 binds with different requirements to distinct populations of Gcn1 molecules, and its ability to disrupt Gcn1-Gcn2 complexes is dependent on a complete RWD and hindered by actin binding.
Title: Gcn1 and actin binding to Yih1: implications for activation of the eIF2 kinase GCN2.

IMPACT bound to the minimal yeast GCN1 segment required for interaction with yeast GCN2 and YIH1 and to native mouse GCN1.
Title: IMPACT, a protein preferentially expressed in the mouse brain, binds GCN1 and inhibits GCN2 activation.

eEF3-related activity of GCN1 influences occupancy of the ribosomal decoding site by uncharged tRNA in starved cells.
Title: Polyribosome binding by GCN1 is required for full activation of eukaryotic translation initiation factor 2{alpha} kinase GCN2 during amino acid starvation.

The following GCN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02060	NM_001181060.3 Latest version!	Saccharomyces cerevisiae S288C Gcn1p (GCN1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GCN1 ( NM_001181060.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011320.3 Saccharomyces cerevisiae S288C Gcn1p (GCN1), partial mRNA.

Price & Availability↑

CloneID	OSi02060
Clone ID Related Accession (Same CDS sequence)	NM_001181060.3
Accession Version	NM_001181060.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Gcn1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). On Jul 30, 2012 this sequence version replaced NM_001181060.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACAGCTA TCTTAAACTG GGAAGATATA TCCCCTGTTT TAGAGAAAGG CACCCGCGAA 
TCACACGTGT CTAAAAGAGT TCCCTTTTTG CAAGATATAT CACAATTAGT TCGTCAAGAA 
ACATTGGAAA AACCTCAATT ATCTGAAATT GCTTTCGTTT TATTGAATAC CTTCACGATT 
TACGAAGACA ATAGGTCTAA AAGTTTGGTA ACTTCAATAT TGCTTGACAT CTTAAATCTA 
GAGCCATGTC TCCTAGAAAA CTTTATCCGA TTCATTTCCG ACGTGGTCAT CAGTAATCCG 
GCTACCAAAG CAGTCGCTGA TTACCTTAAT TTGTTAGATT GGATTAATTC TTTCTTGATT 
TTTGTGTCGC ATAATTCTAA TTTATTTGAA GAGTACATTC CAAAATTGTT GGTCGCCCAT 
TCTTATGCAA CGTTCGGTGT GGAAACTATT CTTGACAATC AAGAAGAAGG CAAGAAATCC 
CAAGATAAGC AGAACCAGCA TAGAAAGAGA ATTCGTTATT GCATTTTCCA AACAACGGTC 
AAAGCATTCC TTAAGTGCTT GAAGGATAAT GACGACAGTA TTTCCTTCAT GAAGATTTCA 
ATTAAAACTG TACTGGAGAG CTACTCTAAG CTGAAGATAA CCAGTGTTGG AGTAGTTATG 
ATAATGGGTG CACTAACTCA GGCTGCACTT CAGTTATTAT CGAGGCAACC AGCTCTGCAC 
TCCGTTTTAA AGGAAAATTC TGCAGAGAAA TATTGCGAAT ATTTGGGCAA AGAAGTGTTT 
TTAGGCAAAA ACCCACCATC TTCCTTTTGC TTAGAGATTG GTTTGAAGCC ATTTTTAAAA 
GAATTTGTTT CACAAGAGTT ATTCATTAAG TTTTTCATCC CTAATATTGA AAAAGCAGTT 
TTAAGATCTC CTGAGGTTGG TTTTTCGATT TTATCTGAGT TATATGCTGG TGTTTCTCCT 
GAAAAAGTAA ATCTCTTAAA TGCCTTTGCT TCCTCGAAAC TGATTAATCA ATACTTCTCG 
TCATTTAAAA GCTCAAAAGA GGTAGTTAGA TCCGTCTCGC TTCAGTCTAT GATAATCTTA 
TTGAGAAAAA TTTCCAATAC TGACACTACC TTGGAAGATT TGACCAAGCT TATTGACGAA 
ATATTTAAGA ACATCAAATC GAACTTAAAT GCAGATTACA AATCACTTAT TTCCAAAATT 
CTCATAGAAA TACCCTTAAC GCACTATGAG GTTTCAGAAA AAATCTGCAA AGGGCTGTCC 
CCATATATTG GTAAAGAAGG TAATGAAGCA GCCTTGACTC TAATGCTTAA CGCTTTTTTT 
GTTCATTATT TTAGTCTGGG CAAACCTATT GAAGACTTGG ATAAAATTAT ATCAGCTGGT 
TTTGCAGATA AAAAACCTGC TTTGAAAAAA TGCTGGTTTG CCGCATTTTT GAACAATTCC 
AATGCTGCCT CTGAAGAGGT GATTTTAAAC TTTATAGACG GTTGTTTGGA ATTTGTGAAA 
GACTCCATCA TACATTATCA GACTCACGGG CATGCATGTA TTCTAGCATC AATTGAATTT 
ACAAACAAAA TTTTGGCATT GGACAATACT GAGCTAAATG ATCGTGTTAT GCAGCTCATA 
GAAACCCTTC CTGAAAATTC TTCGATAGGT GATGCTATCT TAACTGCTGC ATTATCAACA 
GAGCTATCCA TCGAAAACCG TATTCATGCT GTTAATTTAC TACAAGAATT GTTCTACAAG 
AAACCCGAAT TTATTGGGTT TAGCGTGATT GATGCAATTG AAAGGAGAAT GCGTGTCCAA 
GAATTAATAC CTCAACAAAA TACATCCTTT AAATATGTCA CTTCTGTACT ACTGGCCATT 
ACGTCTGAGT TACCTGACAA GGAAGCCTCG ATAAAGGTAT TAATCAATGC TCTGGTCATA 
GCGCAATGGA ATATTTTCAA CATTAAGAAT GGCTGGGCTG GTTTAGTTTT ACGTGCAAGG 
CTTGATCCAG CTGAAGTTGT CAAAGAACAT GCTAGCGTTA TCATGGAGAA GATTCTTGAA 
ATCACAGGTA GTTGTGAATG GATAGATACA ATTTATGGTG CATGCGGTTT GCAAGCAGCT 
GCCTATGCAG CATTTATTCA GCCTAATGAG TTCACACCGA TCCTTTGTAA GACTATCGAA 
GCAGATTTAA CCGCGGATGA TTTCTCACGT TTATCTGAAG AGGACTTCGA AATTTTTGCA 
GGAGAGGAAG GTGTTCTGGT GGTTGACGTC CTGGAAGAAA GCATGAATAA GAAACTTTCC 
AATAAAAATT CTAAAGAGTA CGAAACTCTA ATGTGGGAAC AAAAAATAAG GAAGGAGCAA 
GCCAAAAAGA ATGTGAAAAA ACTTTCAAAA GAGGAACAAG AGCTCGTTAA TGAACAGTTA 
GCGAAAGAAT CTGCAGTTAG ATCACACGTT TCGGAAATTT CCACTCGTTT AAAGCGTGGT 
ATTAGATTGG TATCTGAACT TTCCAAAGCT GCTTGCTTAG TCCAAAATGG CATCGCTACT 
TGGTTCCCCT TGGCAGTCAC AAAGCTTTTG TACCTATGCT CGGAACCAAA TATTTCAAAG 
CTAACAGAGG ATGTAAATAA TGTGTTCTTG CAGCTTTCTC AAAATGTCTC CGAGAGATTA 
GGTAATATCA GACTTTTCTT AGGTTTGGCG ACTTTGCGTG TGCACAATGC TAATGGCATC 
TCGCAAGATT ATTTACAAGA ACCACTGGTT GAATTACTTA CAAGAGTTCT TTTTAGGATC 
AAGTTCGTCT CCAACCAAGC AGCAATAGAT TCCATCAGTT TGACATATAT TTTACCATTG 
TTGATTAATG TTTTAGAAAA GGGTAAGGCG ATTGCATTAA AGAATGCGGA CAAGCCTGTT 
GTTAAGGCTG AGTTTGTTGA AGAAGATGAA GAAGAAGAAC ATCTGTTACT GGCCATGGAA 
ATTATTTCTG TACACGCTGA AGCTTTTGAG GATCCTTCCA TTCCGAGAAT TTCCATTGTT 
GAAGTGTTAC TATCTCTTCT ATCTTTACCC TCAAAAGCGA AGATTGCTAA AGATTGTTTC 
AACGCTCTGT GCCAAAGCAT ATCTGTTGCA CCAAATCAAG AAGACCTTGA TATGATACTA 
TCAAATTTAC TATCACCAAA TCAATTTGTC CGTTCAACAA TATTAGAAAC TCTTGATAAC 
GAATTCGAAT TAGAACCTTT TATGAAATAT TCACCTGAAG TTTTCATTTG CAGATTTGAT 
TCGGATCCTT CCAACCGCGA GATTGCAGAT TTCATTTGGG AGTTCAACAA ATTTGTAGTC 
AACGATGAAT TACTAAAAAG CTTATTCCCA CTATTCAATC AGGATGATAG TGGCTTGAGA 
TTATTTGCGG CAAACGCGTA CGCATTTGGT GCGGTAAGTC TGTTTACCTC TGAAGAGAAC 
TCCTCGAAAG ATTACTTAAA TGATTTGCTG AACTTTTATA AAGAAAAGGC AAAGCCATTG 
GAGCCAATTC TTGATCAATT TGGCTTGGTT CTTGTTTCTG CGAGTGAACA AAAAGATCCA 
TGGCAAGGAA GAAGTACCGT TGCTATTACA TTAAAAATCA TGGCTAAGGC TTTTTCTGCA 
GAGGATGATA CTGTCGTTAA CATTATAAAA TTTTTGGTCG ATGATGGAGG TCTAGTAGAC 
AGAGAGCCTA TTGTTCGTCA AGAAATGAAA GAAGCTGGTG TTGAATTAAT TACGTTACAT 
GGCTCACAAA ACTCGAAGGA TTTAATTCCT ATATTTGAAG AAGCATTAAG CTCCAGTACG 
GACAGTGCCT TAAAAGAGAA CGTTATTATT TTGTATGGTA CATTAGCAAG ACATTTACAG 
CAAAGTGATG CAAGGATTCA CACGATCATT GAAAGATTGC TTTCAACTTT GGATACTCCT 
TCTGCGGATA TTCAACAGGC TGTGTCGGCT TGTATAGCAC CACTAGTTTT CCAGTTCAAA 
CAAAAAGTTG GTGATTACTT GGGTATCCTA ATGGAGAAAC TGCTGAATCC AACTGTTGCT 
TCTTCTATGC GGAAAGGTGC CGCTTGGGGT ATCGCTGGTT TAGTGAAAGG TTACGGTATC 
TCGGCTCTCT CGGAGTTTGA CATTATTCGC AACCTCATCG AAGCTGCAGA AGATAAAAAG 
GAGCCAAAAA GACGTGAATC TGTTGGCTTC TGCTTTCAAT ATTTGTCTGA ATCTCTAGGA 
AAGTTTTTTG AACCATATGT GATAGAAATT CTTCCAAATA TTTTAAAGAA TTTAGGGGAT 
GCTGTTCCTG AAGTTAGAGA TGCAACCGCT CGTGCCACTA AGGCTATAAT GGCGCATACT 
ACAGGTTACG GTGTTAAAAA GTTAATTCCA GTTGCTGTTT CTAATTTGGA TGAAATTGCT 
TGGAGAACTA AGAGGGGCTC TGTTCAATTG TTGGGTAATA TGGCTTATTT AGATCCTACT 
CAATTGTCAG CTTCTTTGTC CACCATTGTC CCAGAAATTG TTGGTGTATT GAACGACTCT 
CACAAAGAGG TGCGTAAGGC CGCTGATGAA TCCTTGAAAA GATTCGGTGA AGTTATCAGA 
AATCCGGAAA TTCAGAAATT GGTGCCCGTA CTTTTGCAAG CTATCGGTGA TCCAACAAAA 
TACACTGAAG AGGCCTTGGA TTCGTTAATT CAAACACAAT TTGTCCATTA TATTGATGGT 
CCTTCACTAG CACTAATTAT TCATATTATT CATCGTGGTA TGCATGATAG ATCTGCCAAC 
ATTAAGAGGA AAGCATGTAA GATCGTGGGC AATATGGCCA TTTTGGTTGA TACCAAAGAT 
CTCATCCCAT ATTTACAACA GCTGATAGAT GAAGTGGAGA TTGCTATGGT GGATCCAGTT 
CCAAATACTA GAGCCACAGC AGCACGTGCT TTGGGTGCTT TGGTAGAAAG GTTAGGTGAA 
GAGCAATTCC CAGATTTGAT TCCTCGTCTA CTAGATACCT TAAGTGACGA ATCAAAATCT 
GGTGATCGTC TCGGTTCTGC TCAAGCTCTA GCTGAAGTTA TTAGTGGCTT GGGCTTGACC 
AAGTTGGATG AGATGTTACC AACCATTTTA GCTGGTGTAA CCAATTTTCG TGCTTATATC 
AGGGAAGGAT TCATGCCTTT GCTGCTTTTC CTTCCTGTTT GTTTTGGATC ACAATTTGCT 
CCATACATTA ATCAGATTAT TCAGCCTATT CTTTCCGGAT TGGCCGATAA TGATGAAAAT 
ATTCGCGATA CTGCTTTGAA GGCTGGTAAA TTAATTGTCA AAAACTACGC TACAAAGGCC 
GTTGATTTGT TGTTGCCTGA ATTAGAAAGG GGTATGTTCG ATGAAAATGA CAGAATTCGC 
TTATCTTCTG TTCAATTAAC CGGAGAACTA TTGTTCCAAG TTACTGGTAT TTCCTCCAGG 
AACGAATTTT CTGAGGAAGA TGGTGATCAT AATGGTGAAT TCTCTGGTAA ATTGGTCGAT 
GTACTTGGCC AAGACCGTCG TGATAGAATT TTAGCCGCAT TATTTGTATG CAGGAACGAC 
ACTTCTGGTA TCGTACGTGC TACGACGGTT GACATTTGGA AGGCATTGGT TCCAAATACT 
CCAAGAGCTG TGAAAGAGAT CCTTCCAACA TTGACTGGTA TGATAGTCAC TCACTTGGCT 
TCATCATCCA ATGTATTACG CAACATTGCT GCTCAGACCT TAGGTGATCT TGTCCGTCGT 
GTAGGTGGTA ATGCTTTGTC CCAACTGTTA CCAAGTTTGG AGGAATCTTT GATAGAAACA 
TCAAACTCAG ATTCGAGACA AGGTGTTTGT ATTGCTCTTT ATGAGTTAAT TGAATCGGCT 
TCTACGGAAA CGATATCACA GTTCCAATCT ACCATCGTTA ACATTATTCG TACGGCATTA 
ATTGATGAGT CGGCTACTGT CAGAGAAGCG GCTGCATTAT CTTTTGATGT ATTCCAAGAT 
GTTGTAGGAA AAACTGCTGT TGATGAAGTT TTACCATATT TGTTGCATAT GCTTGAATCT 
TCTGATAATT CTGACTTTGC TTTGTTAGGT TTACAAGAAA TTATGTCGAA GAAGTCCGAC 
GTAATCTTCC CAATTTTAAT TCCAACCCTA TTAGCCCCTC CAATAGACGC CTTCAGGGCT 
TCTGCTTTAG GTTCTTTGGC GGAAGTTGCT GGCTCAGCCT TATACAAGCG TTTATCAATT 
ATAATCAACG CACTAGTGGA TGCAATCATA GGTACTTCTG AAGATGAATC GACCAAGGGT 
GCATTAGAAC TTGCATTAGA CAGGGTATTC TTATCTGTGA ATGATGACGA AGGTCTTCAC 
CCATTACTTC AACAGATTAT GTCACTACTA AAGAGTGATA ATATAGAGAA ACGCATAGCT 
GTTTTAGAAC GTTTGCCAAA TTTCTTCGAT AAGACTGTTC TTGATTTTGA TGTCTATATT 
CCGAACTTTG TCTCTCACGC AATTTTATCA TTAGATGATG AAGATCAAAG GGTTGTTAAT 
GGTAACTTCA ATGCTTTGTC TACTTTGTTG AAGAAGGTTG ATAAGCCCAC CTTAGAAAAA 
TTAGTTAAGC CTGCTAAACA GTCATTGGCA TTAACAGGCA GGCAAGGTCA AGATGTCGCT 
GCATTTAAGC TTCCAAGAGG CCCTAACTGT GTTTTGCCTA TTTTCTTGCA TGGTTTGATG 
TATGGTTCGA ATGATGAAAG GGAAGAATCT GCATTAGCCA TTGCTGACGT TGTTTCGAAG 
ACCCCTGCCG CTAACTTGAA GCCATTTGTG AGCGTAATTA CTGGTCCATT AATTCGTGTC 
GTGGGTGAAA GATTTAGTAG TGATATCAAA GCAGCAATTT TATTTGCACT TAATGTGCTA 
TTCATTAAGA TTCCAATGTT CTTGAGGCCT TTTATCCCTC AATTACAAAG AACATTTGTT 
AAATCCTTGT CTGACGCTAC CAATGAAACG TTACGTCTCC GCGCCGCAAA GGCTCTTGGT 
GCCCTGATTG AACATCAGCC TCGTGTTGAC CCTCTAGTCA TTGAACTGGT GACAGGTGCC 
AAGCAAGCCA CAGATGAAGG TGTCAAGACT GCGATGCTTA AAGCTTTACT GGAAGTTATT 
ATGAAGGCTG GTTCCAAATT AAACGAAAAT TCTAAGACAA ACATTGTCAA TTTAGTTGAG 
GAAGAAATGT TGGGTAGCAA TGACAAATTG GCAGTTGCTT ACGCTAAATT AATCGGATCG 
TTATCAGAGA TTTTGTCGAA CGACGAAGCC CACAAGATAT TGCAAGACAA GGTTTTGAAT 
GCAGATCTAG ATGGAGAAAC CGGTAAGTTT GCTATTCTGA CTTTGAATTC CTTTTTGAAA 
GATGCACCAA CACATATATT CAATACGGGC TTGATAGACG AATTTGTAAG TTACATTTTG 
AATGCAATCC GTTCCCCTGA TGTTTACTTC GGAGAAAATG GTACCATTGC TGCTGGTAAA 
TTACTTTTAT TAGAAGGAGA AAAGAGGTCT CCATTTGTTA AAAAGGATGC TGCAGAACCA 
TTCAAAATTG GCGATGAAAA CATCAATCTG TTAATTAATG AGTTGAGCAA AGCTGTCTTA 
CAACCAGCCA GTAATTCTAC GGATGTAAGA AGGTTGGCCT TGGTGGTTAT AAGAACACTA 
GCCAGATTCA AATTTGACGA GTGCATTAAG CAATACTTCG ATGTAGTAGG ACCATCTGTA 
TTTTCTTGCT TGCGTGATCC TGTTATCCCA ATTAAGCTCG CAGCAGAAAA AGCATATTTA 
GCTTTGTTCA AATTGGTTGA AGAAGATGAC ATGCATACTT TCAACGAGTG GTTTGCTAAA 
ATTTCAGATC GCGGTAACAG CATCGAAACT GTCACAGGTA CTACAATTCA ATTACGGTCT 
GTTGGGGACT ATACCAAGAG GGTTGGTAAA AGGTTAGCAA ATGTCGAAAG AGAAAGGATT 
GCTGCCGGAG GAGACGCGGA AACAATGTTT AGTGACAGAT TTGAAGATGA AAGAGAAATA 
TGGGCTGTCG GAGGTGTTGA ATTAACCACT GATATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011320.3
CDS	1..8019
Translation	MTAILNWEDISPVLEKGTRESHVSKRVPFLQDISQLVRQETLEKPQLSEIAFVLLNTFTIYEDNRSKSLVTSILLDILNLEPCLLENFIRFISDVVISNPATKAVADYLNLLDWINSFLIFVSHNSNLFEEYIPKLLVAHSYATFGVETILDNQEEGKKSQDKQNQHRKRIRYCIFQTTVKAFLKCLKDNDDSISFMKISIKTVLESYSKLKITSVGVVMIMGALTQAALQLLSRQPALHSVLKENSAEKYCEYLGKEVFLGKNPPSSFCLEIGLKPFLKEFVSQELFIKFFIPNIEKAVLRSPEVGFSILSELYAGVSPEKVNLLNAFASSKLINQYFSSFKSSKEVVRSVSLQSMIILLRKISNTDTTLEDLTKLIDEIFKNIKSNLNADYKSLISKILIEIPLTHYEVSEKICKGLSPYIGKEGNEAALTLMLNAFFVHYFSLGKPIEDLDKIISAGFADKKPALKKCWFAAFLNNSNAASEEVILNFIDGCLEFVKDSIIHYQTHGHACILASIEFTNKILALDNTELNDRVMQLIETLPENSSIGDAILTAALSTELSIENRIHAVNLLQELFYKKPEFIGFSVIDAIERRMRVQELIPQQNTSFKYVTSVLLAITSELPDKEASIKVLINALVIAQWNIFNIKNGWAGLVLRARLDPAEVVKEHASVIMEKILEITGSCEWIDTIYGACGLQAAAYAAFIQPNEFTPILCKTIEADLTADDFSRLSEEDFEIFAGEEGVLVVDVLEESMNKKLSNKNSKEYETLMWEQKIRKEQAKKNVKKLSKEEQELVNEQLAKESAVRSHVSEISTRLKRGIRLVSELSKAACLVQNGIATWFPLAVTKLLYLCSEPNISKLTEDVNNVFLQLSQNVSERLGNIRLFLGLATLRVHNANGISQDYLQEPLVELLTRVLFRIKFVSNQAAIDSISLTYILPLLINVLEKGKAIALKNADKPVVKAEFVEEDEEEEHLLLAMEIISVHAEAFEDPSIPRISIVEVLLSLLSLPSKAKIAKDCFNALCQSISVAPNQEDLDMILSNLLSPNQFVRSTILETLDNEFELEPFMKYSPEVFICRFDSDPSNREIADFIWEFNKFVVNDELLKSLFPLFNQDDSGLRLFAANAYAFGAVSLFTSEENSSKDYLNDLLNFYKEKAKPLEPILDQFGLVLVSASEQKDPWQGRSTVAITLKIMAKAFSAEDDTVVNIIKFLVDDGGLVDREPIVRQEMKEAGVELITLHGSQNSKDLIPIFEEALSSSTDSALKENVIILYGTLARHLQQSDARIHTIIERLLSTLDTPSADIQQAVSACIAPLVFQFKQKVGDYLGILMEKLLNPTVASSMRKGAAWGIAGLVKGYGISALSEFDIIRNLIEAAEDKKEPKRRESVGFCFQYLSESLGKFFEPYVIEILPNILKNLGDAVPEVRDATARATKAIMAHTTGYGVKKLIPVAVSNLDEIAWRTKRGSVQLLGNMAYLDPTQLSASLSTIVPEIVGVLNDSHKEVRKAADESLKRFGEVIRNPEIQKLVPVLLQAIGDPTKYTEEALDSLIQTQFVHYIDGPSLALIIHIIHRGMHDRSANIKRKACKIVGNMAILVDTKDLIPYLQQLIDEVEIAMVDPVPNTRATAARALGALVERLGEEQFPDLIPRLLDTLSDESKSGDRLGSAQALAEVISGLGLTKLDEMLPTILAGVTNFRAYIREGFMPLLLFLPVCFGSQFAPYINQIIQPILSGLADNDENIRDTALKAGKLIVKNYATKAVDLLLPELERGMFDENDRIRLSSVQLTGELLFQVTGISSRNEFSEEDGDHNGEFSGKLVDVLGQDRRDRILAALFVCRNDTSGIVRATTVDIWKALVPNTPRAVKEILPTLTGMIVTHLASSSNVLRNIAAQTLGDLVRRVGGNALSQLLPSLEESLIETSNSDSRQGVCIALYELIESASTETISQFQSTIVNIIRTALIDESATVREAAALSFDVFQDVVGKTAVDEVLPYLLHMLESSDNSDFALLGLQEIMSKKSDVIFPILIPTLLAPPIDAFRASALGSLAEVAGSALYKRLSIIINALVDAIIGTSEDESTKGALELALDRVFLSVNDDEGLHPLLQQIMSLLKSDNIEKRIAVLERLPNFFDKTVLDFDVYIPNFVSHAILSLDDEDQRVVNGNFNALSTLLKKVDKPTLEKLVKPAKQSLALTGRQGQDVAAFKLPRGPNCVLPIFLHGLMYGSNDEREESALAIADVVSKTPAANLKPFVSVITGPLIRVVGERFSSDIKAAILFALNVLFIKIPMFLRPFIPQLQRTFVKSLSDATNETLRLRAAKALGALIEHQPRVDPLVIELVTGAKQATDEGVKTAMLKALLEVIMKAGSKLNENSKTNIVNLVEEEMLGSNDKLAVAYAKLIGSLSEILSNDEAHKILQDKVLNADLDGETGKFAILTLNSFLKDAPTHIFNTGLIDEFVSYILNAIRSPDVYFGENGTIAAGKLLLLEGEKRSPFVKKDAAEPFKIGDENINLLINELSKAVLQPASNSTDVRRLALVVIRTLARFKFDECIKQYFDVVGPSVFSCLRDPVIPIKLAAEKAYLALFKLVEEDDMHTFNEWFAKISDRGNSIETVTGTTIQLRSVGDYTKRVGKRLANVERERIAAGGDAETMFSDRFEDEREIWAVGGVELTTDI

Target ORF information:

RefSeq Version	NM_001181060.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Gcn1p (GCN1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181060.3

ORF Insert Sequence:

ATGACAGCTA TCTTAAACTG GGAAGATATA TCCCCTGTTT TAGAGAAAGG CACCCGCGAA 
TCACACGTGT CTAAAAGAGT TCCCTTTTTG CAAGATATAT CACAATTAGT TCGTCAAGAA 
ACATTGGAAA AACCTCAATT ATCTGAAATT GCTTTCGTTT TATTGAATAC CTTCACGATT 
TACGAAGACA ATAGGTCTAA AAGTTTGGTA ACTTCAATAT TGCTTGACAT CTTAAATCTA 
GAGCCATGTC TCCTAGAAAA CTTTATCCGA TTCATTTCCG ACGTGGTCAT CAGTAATCCG 
GCTACCAAAG CAGTCGCTGA TTACCTTAAT TTGTTAGATT GGATTAATTC TTTCTTGATT 
TTTGTGTCGC ATAATTCTAA TTTATTTGAA GAGTACATTC CAAAATTGTT GGTCGCCCAT 
TCTTATGCAA CGTTCGGTGT GGAAACTATT CTTGACAATC AAGAAGAAGG CAAGAAATCC 
CAAGATAAGC AGAACCAGCA TAGAAAGAGA ATTCGTTATT GCATTTTCCA AACAACGGTC 
AAAGCATTCC TTAAGTGCTT GAAGGATAAT GACGACAGTA TTTCCTTCAT GAAGATTTCA 
ATTAAAACTG TACTGGAGAG CTACTCTAAG CTGAAGATAA CCAGTGTTGG AGTAGTTATG 
ATAATGGGTG CACTAACTCA GGCTGCACTT CAGTTATTAT CGAGGCAACC AGCTCTGCAC 
TCCGTTTTAA AGGAAAATTC TGCAGAGAAA TATTGCGAAT ATTTGGGCAA AGAAGTGTTT 
TTAGGCAAAA ACCCACCATC TTCCTTTTGC TTAGAGATTG GTTTGAAGCC ATTTTTAAAA 
GAATTTGTTT CACAAGAGTT ATTCATTAAG TTTTTCATCC CTAATATTGA AAAAGCAGTT 
TTAAGATCTC CTGAGGTTGG TTTTTCGATT TTATCTGAGT TATATGCTGG TGTTTCTCCT 
GAAAAAGTAA ATCTCTTAAA TGCCTTTGCT TCCTCGAAAC TGATTAATCA ATACTTCTCG 
TCATTTAAAA GCTCAAAAGA GGTAGTTAGA TCCGTCTCGC TTCAGTCTAT GATAATCTTA 
TTGAGAAAAA TTTCCAATAC TGACACTACC TTGGAAGATT TGACCAAGCT TATTGACGAA 
ATATTTAAGA ACATCAAATC GAACTTAAAT GCAGATTACA AATCACTTAT TTCCAAAATT 
CTCATAGAAA TACCCTTAAC GCACTATGAG GTTTCAGAAA AAATCTGCAA AGGGCTGTCC 
CCATATATTG GTAAAGAAGG TAATGAAGCA GCCTTGACTC TAATGCTTAA CGCTTTTTTT 
GTTCATTATT TTAGTCTGGG CAAACCTATT GAAGACTTGG ATAAAATTAT ATCAGCTGGT 
TTTGCAGATA AAAAACCTGC TTTGAAAAAA TGCTGGTTTG CCGCATTTTT GAACAATTCC 
AATGCTGCCT CTGAAGAGGT GATTTTAAAC TTTATAGACG GTTGTTTGGA ATTTGTGAAA 
GACTCCATCA TACATTATCA GACTCACGGG CATGCATGTA TTCTAGCATC AATTGAATTT 
ACAAACAAAA TTTTGGCATT GGACAATACT GAGCTAAATG ATCGTGTTAT GCAGCTCATA 
GAAACCCTTC CTGAAAATTC TTCGATAGGT GATGCTATCT TAACTGCTGC ATTATCAACA 
GAGCTATCCA TCGAAAACCG TATTCATGCT GTTAATTTAC TACAAGAATT GTTCTACAAG 
AAACCCGAAT TTATTGGGTT TAGCGTGATT GATGCAATTG AAAGGAGAAT GCGTGTCCAA 
GAATTAATAC CTCAACAAAA TACATCCTTT AAATATGTCA CTTCTGTACT ACTGGCCATT 
ACGTCTGAGT TACCTGACAA GGAAGCCTCG ATAAAGGTAT TAATCAATGC TCTGGTCATA 
GCGCAATGGA ATATTTTCAA CATTAAGAAT GGCTGGGCTG GTTTAGTTTT ACGTGCAAGG 
CTTGATCCAG CTGAAGTTGT CAAAGAACAT GCTAGCGTTA TCATGGAGAA GATTCTTGAA 
ATCACAGGTA GTTGTGAATG GATAGATACA ATTTATGGTG CATGCGGTTT GCAAGCAGCT 
GCCTATGCAG CATTTATTCA GCCTAATGAG TTCACACCGA TCCTTTGTAA GACTATCGAA 
GCAGATTTAA CCGCGGATGA TTTCTCACGT TTATCTGAAG AGGACTTCGA AATTTTTGCA 
GGAGAGGAAG GTGTTCTGGT GGTTGACGTC CTGGAAGAAA GCATGAATAA GAAACTTTCC 
AATAAAAATT CTAAAGAGTA CGAAACTCTA ATGTGGGAAC AAAAAATAAG GAAGGAGCAA 
GCCAAAAAGA ATGTGAAAAA ACTTTCAAAA GAGGAACAAG AGCTCGTTAA TGAACAGTTA 
GCGAAAGAAT CTGCAGTTAG ATCACACGTT TCGGAAATTT CCACTCGTTT AAAGCGTGGT 
ATTAGATTGG TATCTGAACT TTCCAAAGCT GCTTGCTTAG TCCAAAATGG CATCGCTACT 
TGGTTCCCCT TGGCAGTCAC AAAGCTTTTG TACCTATGCT CGGAACCAAA TATTTCAAAG 
CTAACAGAGG ATGTAAATAA TGTGTTCTTG CAGCTTTCTC AAAATGTCTC CGAGAGATTA 
GGTAATATCA GACTTTTCTT AGGTTTGGCG ACTTTGCGTG TGCACAATGC TAATGGCATC 
TCGCAAGATT ATTTACAAGA ACCACTGGTT GAATTACTTA CAAGAGTTCT TTTTAGGATC 
AAGTTCGTCT CCAACCAAGC AGCAATAGAT TCCATCAGTT TGACATATAT TTTACCATTG 
TTGATTAATG TTTTAGAAAA GGGTAAGGCG ATTGCATTAA AGAATGCGGA CAAGCCTGTT 
GTTAAGGCTG AGTTTGTTGA AGAAGATGAA GAAGAAGAAC ATCTGTTACT GGCCATGGAA 
ATTATTTCTG TACACGCTGA AGCTTTTGAG GATCCTTCCA TTCCGAGAAT TTCCATTGTT 
GAAGTGTTAC TATCTCTTCT ATCTTTACCC TCAAAAGCGA AGATTGCTAA AGATTGTTTC 
AACGCTCTGT GCCAAAGCAT ATCTGTTGCA CCAAATCAAG AAGACCTTGA TATGATACTA 
TCAAATTTAC TATCACCAAA TCAATTTGTC CGTTCAACAA TATTAGAAAC TCTTGATAAC 
GAATTCGAAT TAGAACCTTT TATGAAATAT TCACCTGAAG TTTTCATTTG CAGATTTGAT 
TCGGATCCTT CCAACCGCGA GATTGCAGAT TTCATTTGGG AGTTCAACAA ATTTGTAGTC 
AACGATGAAT TACTAAAAAG CTTATTCCCA CTATTCAATC AGGATGATAG TGGCTTGAGA 
TTATTTGCGG CAAACGCGTA CGCATTTGGT GCGGTAAGTC TGTTTACCTC TGAAGAGAAC 
TCCTCGAAAG ATTACTTAAA TGATTTGCTG AACTTTTATA AAGAAAAGGC AAAGCCATTG 
GAGCCAATTC TTGATCAATT TGGCTTGGTT CTTGTTTCTG CGAGTGAACA AAAAGATCCA 
TGGCAAGGAA GAAGTACCGT TGCTATTACA TTAAAAATCA TGGCTAAGGC TTTTTCTGCA 
GAGGATGATA CTGTCGTTAA CATTATAAAA TTTTTGGTCG ATGATGGAGG TCTAGTAGAC 
AGAGAGCCTA TTGTTCGTCA AGAAATGAAA GAAGCTGGTG TTGAATTAAT TACGTTACAT 
GGCTCACAAA ACTCGAAGGA TTTAATTCCT ATATTTGAAG AAGCATTAAG CTCCAGTACG 
GACAGTGCCT TAAAAGAGAA CGTTATTATT TTGTATGGTA CATTAGCAAG ACATTTACAG 
CAAAGTGATG CAAGGATTCA CACGATCATT GAAAGATTGC TTTCAACTTT GGATACTCCT 
TCTGCGGATA TTCAACAGGC TGTGTCGGCT TGTATAGCAC CACTAGTTTT CCAGTTCAAA 
CAAAAAGTTG GTGATTACTT GGGTATCCTA ATGGAGAAAC TGCTGAATCC AACTGTTGCT 
TCTTCTATGC GGAAAGGTGC CGCTTGGGGT ATCGCTGGTT TAGTGAAAGG TTACGGTATC 
TCGGCTCTCT CGGAGTTTGA CATTATTCGC AACCTCATCG AAGCTGCAGA AGATAAAAAG 
GAGCCAAAAA GACGTGAATC TGTTGGCTTC TGCTTTCAAT ATTTGTCTGA ATCTCTAGGA 
AAGTTTTTTG AACCATATGT GATAGAAATT CTTCCAAATA TTTTAAAGAA TTTAGGGGAT 
GCTGTTCCTG AAGTTAGAGA TGCAACCGCT CGTGCCACTA AGGCTATAAT GGCGCATACT 
ACAGGTTACG GTGTTAAAAA GTTAATTCCA GTTGCTGTTT CTAATTTGGA TGAAATTGCT 
TGGAGAACTA AGAGGGGCTC TGTTCAATTG TTGGGTAATA TGGCTTATTT AGATCCTACT 
CAATTGTCAG CTTCTTTGTC CACCATTGTC CCAGAAATTG TTGGTGTATT GAACGACTCT 
CACAAAGAGG TGCGTAAGGC CGCTGATGAA TCCTTGAAAA GATTCGGTGA AGTTATCAGA 
AATCCGGAAA TTCAGAAATT GGTGCCCGTA CTTTTGCAAG CTATCGGTGA TCCAACAAAA 
TACACTGAAG AGGCCTTGGA TTCGTTAATT CAAACACAAT TTGTCCATTA TATTGATGGT 
CCTTCACTAG CACTAATTAT TCATATTATT CATCGTGGTA TGCATGATAG ATCTGCCAAC 
ATTAAGAGGA AAGCATGTAA GATCGTGGGC AATATGGCCA TTTTGGTTGA TACCAAAGAT 
CTCATCCCAT ATTTACAACA GCTGATAGAT GAAGTGGAGA TTGCTATGGT GGATCCAGTT 
CCAAATACTA GAGCCACAGC AGCACGTGCT TTGGGTGCTT TGGTAGAAAG GTTAGGTGAA 
GAGCAATTCC CAGATTTGAT TCCTCGTCTA CTAGATACCT TAAGTGACGA ATCAAAATCT 
GGTGATCGTC TCGGTTCTGC TCAAGCTCTA GCTGAAGTTA TTAGTGGCTT GGGCTTGACC 
AAGTTGGATG AGATGTTACC AACCATTTTA GCTGGTGTAA CCAATTTTCG TGCTTATATC 
AGGGAAGGAT TCATGCCTTT GCTGCTTTTC CTTCCTGTTT GTTTTGGATC ACAATTTGCT 
CCATACATTA ATCAGATTAT TCAGCCTATT CTTTCCGGAT TGGCCGATAA TGATGAAAAT 
ATTCGCGATA CTGCTTTGAA GGCTGGTAAA TTAATTGTCA AAAACTACGC TACAAAGGCC 
GTTGATTTGT TGTTGCCTGA ATTAGAAAGG GGTATGTTCG ATGAAAATGA CAGAATTCGC 
TTATCTTCTG TTCAATTAAC CGGAGAACTA TTGTTCCAAG TTACTGGTAT TTCCTCCAGG 
AACGAATTTT CTGAGGAAGA TGGTGATCAT AATGGTGAAT TCTCTGGTAA ATTGGTCGAT 
GTACTTGGCC AAGACCGTCG TGATAGAATT TTAGCCGCAT TATTTGTATG CAGGAACGAC 
ACTTCTGGTA TCGTACGTGC TACGACGGTT GACATTTGGA AGGCATTGGT TCCAAATACT 
CCAAGAGCTG TGAAAGAGAT CCTTCCAACA TTGACTGGTA TGATAGTCAC TCACTTGGCT 
TCATCATCCA ATGTATTACG CAACATTGCT GCTCAGACCT TAGGTGATCT TGTCCGTCGT 
GTAGGTGGTA ATGCTTTGTC CCAACTGTTA CCAAGTTTGG AGGAATCTTT GATAGAAACA 
TCAAACTCAG ATTCGAGACA AGGTGTTTGT ATTGCTCTTT ATGAGTTAAT TGAATCGGCT 
TCTACGGAAA CGATATCACA GTTCCAATCT ACCATCGTTA ACATTATTCG TACGGCATTA 
ATTGATGAGT CGGCTACTGT CAGAGAAGCG GCTGCATTAT CTTTTGATGT ATTCCAAGAT 
GTTGTAGGAA AAACTGCTGT TGATGAAGTT TTACCATATT TGTTGCATAT GCTTGAATCT 
TCTGATAATT CTGACTTTGC TTTGTTAGGT TTACAAGAAA TTATGTCGAA GAAGTCCGAC 
GTAATCTTCC CAATTTTAAT TCCAACCCTA TTAGCCCCTC CAATAGACGC CTTCAGGGCT 
TCTGCTTTAG GTTCTTTGGC GGAAGTTGCT GGCTCAGCCT TATACAAGCG TTTATCAATT 
ATAATCAACG CACTAGTGGA TGCAATCATA GGTACTTCTG AAGATGAATC GACCAAGGGT 
GCATTAGAAC TTGCATTAGA CAGGGTATTC TTATCTGTGA ATGATGACGA AGGTCTTCAC 
CCATTACTTC AACAGATTAT GTCACTACTA AAGAGTGATA ATATAGAGAA ACGCATAGCT 
GTTTTAGAAC GTTTGCCAAA TTTCTTCGAT AAGACTGTTC TTGATTTTGA TGTCTATATT 
CCGAACTTTG TCTCTCACGC AATTTTATCA TTAGATGATG AAGATCAAAG GGTTGTTAAT 
GGTAACTTCA ATGCTTTGTC TACTTTGTTG AAGAAGGTTG ATAAGCCCAC CTTAGAAAAA 
TTAGTTAAGC CTGCTAAACA GTCATTGGCA TTAACAGGCA GGCAAGGTCA AGATGTCGCT 
GCATTTAAGC TTCCAAGAGG CCCTAACTGT GTTTTGCCTA TTTTCTTGCA TGGTTTGATG 
TATGGTTCGA ATGATGAAAG GGAAGAATCT GCATTAGCCA TTGCTGACGT TGTTTCGAAG 
ACCCCTGCCG CTAACTTGAA GCCATTTGTG AGCGTAATTA CTGGTCCATT AATTCGTGTC 
GTGGGTGAAA GATTTAGTAG TGATATCAAA GCAGCAATTT TATTTGCACT TAATGTGCTA 
TTCATTAAGA TTCCAATGTT CTTGAGGCCT TTTATCCCTC AATTACAAAG AACATTTGTT 
AAATCCTTGT CTGACGCTAC CAATGAAACG TTACGTCTCC GCGCCGCAAA GGCTCTTGGT 
GCCCTGATTG AACATCAGCC TCGTGTTGAC CCTCTAGTCA TTGAACTGGT GACAGGTGCC 
AAGCAAGCCA CAGATGAAGG TGTCAAGACT GCGATGCTTA AAGCTTTACT GGAAGTTATT 
ATGAAGGCTG GTTCCAAATT AAACGAAAAT TCTAAGACAA ACATTGTCAA TTTAGTTGAG 
GAAGAAATGT TGGGTAGCAA TGACAAATTG GCAGTTGCTT ACGCTAAATT AATCGGATCG 
TTATCAGAGA TTTTGTCGAA CGACGAAGCC CACAAGATAT TGCAAGACAA GGTTTTGAAT 
GCAGATCTAG ATGGAGAAAC CGGTAAGTTT GCTATTCTGA CTTTGAATTC CTTTTTGAAA 
GATGCACCAA CACATATATT CAATACGGGC TTGATAGACG AATTTGTAAG TTACATTTTG 
AATGCAATCC GTTCCCCTGA TGTTTACTTC GGAGAAAATG GTACCATTGC TGCTGGTAAA 
TTACTTTTAT TAGAAGGAGA AAAGAGGTCT CCATTTGTTA AAAAGGATGC TGCAGAACCA 
TTCAAAATTG GCGATGAAAA CATCAATCTG TTAATTAATG AGTTGAGCAA AGCTGTCTTA 
CAACCAGCCA GTAATTCTAC GGATGTAAGA AGGTTGGCCT TGGTGGTTAT AAGAACACTA 
GCCAGATTCA AATTTGACGA GTGCATTAAG CAATACTTCG ATGTAGTAGG ACCATCTGTA 
TTTTCTTGCT TGCGTGATCC TGTTATCCCA ATTAAGCTCG CAGCAGAAAA AGCATATTTA 
GCTTTGTTCA AATTGGTTGA AGAAGATGAC ATGCATACTT TCAACGAGTG GTTTGCTAAA 
ATTTCAGATC GCGGTAACAG CATCGAAACT GTCACAGGTA CTACAATTCA ATTACGGTCT 
GTTGGGGACT ATACCAAGAG GGTTGGTAAA AGGTTAGCAA ATGTCGAAAG AGAAAGGATT 
GCTGCCGGAG GAGACGCGGA AACAATGTTT AGTGACAGAT TTGAAGATGA AAGAGAAATA 
TGGGCTGTCG GAGGTGTTGA ATTAACCACT GATATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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GCN1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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