Chfr cDNA ORF clone, Mus musculus(house mouse)

The following Chfr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chfr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu16350 NM_001289579.1
Latest version!
Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OMu16449 NM_001289578.1
Latest version!
Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OMu16462 NM_001289580.1
Latest version!
Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OMu16505 NM_172717.4
Latest version!
Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $391.30
$559.00
OMu17144 NM_001289577.1
Latest version!
Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OMu16350 XM_011249446.2
Latest version!
Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OMu16350 XM_011249445.2
Latest version!
Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OMu35453 XM_006534915.1
Latest version!
Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OMu88879 XM_030254420.1
Latest version!
Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OMu88880 XM_030254421.1
Latest version!
Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OMu16462 XM_030254418.1
Latest version!
Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OMu16462 XM_030254419.1
Latest version!
Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu16350
    Clone ID Related Accession (Same CDS sequence) XM_011249446.2 , XM_011249445.2 , NM_001289579.1
    Accession Version NM_001289579.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase CHFR isoform 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC118260.7 and AK169182.1. On Jan 24, 2014 this sequence version replaced XM_006534918.1. Transcript Variant: This variant (4) contains an alternate exon in the 5' region and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (4) has a shorter N-terminus than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK169182.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
    CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA
    GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA
    AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA
    CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC
    TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA
    GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC
    CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA
    CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG
    CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC
    TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA
    TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA
    AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA
    GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC
    AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT
    CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA
    CCTTCAACAT CTGCCAGCTT GCCAACAGCA GCCCCGGATT ACATGTGCCC TCTTCAAGGA
    AGCCATGCCA TATGCACCTG CTGCTTCCAG CCTATGCCTG ACCGGAGAGC TGAACGGGAG
    CAGGATCCCC GCGTCGCCCC TCAGCAGTGT GCGGTGTGCC TGCAGCCCTT CTGCCACCTG
    TACTGGGGCT GCACGAGGAC TGGCTGCTTT GGCTGCTTGG CCCCATTCTG TGAGCTCAAC
    CTGGGGGACA AGTGCTTGGA TGGAGTGCTG AACAATAACA ATTATGAATC GGACATCCTG
    AAGAATTACC TGGCAACCAG GGGTCTGACA TGGAAAAGTG TGTTGACAGA GAGTCTCCTG
    GCTCTGCAGC GAGGTGTATT TATGCTGTCT GATTACAGAA TCACTGGAAA TACTGTGCTG
    TGTTACTGCT GTGGTCTGCG TAGCTTCCGA GAGCTGACCT ACCAGTATCG TCAGAACATT
    CCTGCTTCTG AGTTGCCAGT GACTGTAACA TCCCGTCCTG ACTGCTACTG GGGCCGTAAC
    TGTCGCACTC AGGTGAAGGC TCACCATGCA ATGAAATTCA ATCACATCTG TGAGCAAACA
    AGGTTCAAGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001276508.1
    CDS610..2184
    Translation

    Target ORF information:

    RefSeq Version NM_001289579.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001289579.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
    CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA
    GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA
    AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA
    CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC
    TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA
    GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC
    CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA
    CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG
    CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC
    TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA
    TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA
    AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA
    GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC
    AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT
    CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA
    CCTTCAACAT CTGCCAGCTT GCCAACAGCA GCCCCGGATT ACATGTGCCC TCTTCAAGGA
    AGCCATGCCA TATGCACCTG CTGCTTCCAG CCTATGCCTG ACCGGAGAGC TGAACGGGAG
    CAGGATCCCC GCGTCGCCCC TCAGCAGTGT GCGGTGTGCC TGCAGCCCTT CTGCCACCTG
    TACTGGGGCT GCACGAGGAC TGGCTGCTTT GGCTGCTTGG CCCCATTCTG TGAGCTCAAC
    CTGGGGGACA AGTGCTTGGA TGGAGTGCTG AACAATAACA ATTATGAATC GGACATCCTG
    AAGAATTACC TGGCAACCAG GGGTCTGACA TGGAAAAGTG TGTTGACAGA GAGTCTCCTG
    GCTCTGCAGC GAGGTGTATT TATGCTGTCT GATTACAGAA TCACTGGAAA TACTGTGCTG
    TGTTACTGCT GTGGTCTGCG TAGCTTCCGA GAGCTGACCT ACCAGTATCG TCAGAACATT
    CCTGCTTCTG AGTTGCCAGT GACTGTAACA TCCCGTCCTG ACTGCTACTG GGGCCGTAAC
    TGTCGCACTC AGGTGAAGGC TCACCATGCA ATGAAATTCA ATCACATCTG TGAGCAAACA
    AGGTTCAAGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16449
    Clone ID Related Accession (Same CDS sequence) NM_001289578.1
    Accession Version NM_001289578.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1779bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase CHFR isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC118260.7 and AK147892.1. On Jan 24, 2014 this sequence version replaced XM_006534917.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK147892.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAGCTAC ACGGGGAAGA GCAGCCGCCG CCGCCGCAGG AACCCTGGGG GAGGCTTCTT 
    CGTCTAGGCG CAGAGGAGGA CGAACCCCAG ATCCTTCTTT GGAAACGCGA GTGGACCATC
    GGAAGGAGGA GAGGCTGTGA CCTCTCTTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC
    TGTAAACTTA CAGTGGATGA AATATCTGGT GAGGTGACAC TGGAAGACAC CAGCACCAAT
    GGAACAGTGA TCAATAAGCT GCAAGTTGTT AAGAAGCAGA CTTACCCTTT ACAGAGCGGG
    GATATCATCT ATTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT
    GAATCTTTAA GTGGCAAACA GAGCTTAACT CAAGACTCCC TTGGACCTGG GAACACAAGC
    ATCTCCCCAA AAGGACGCAG TTCACTTGTT GCAAATGGCG AACTCTCTAG CCTTTCTCCA
    GTTTTCCAAG ACAAAGAAGC ATCCTTTTCT TTGCTGGAAA GTAAAGACCA TGAGGAATTG
    GAGCCTGCCA AAAAAAAGAT GAAAGGAGAT GGGGAACTTG ACACGAACCT CCAGTTATTA
    GTTTCAGGCC AGCGTGGAAA TGCCCAAACC TCAAGTGAAG ATGTCAAAGA TGCCTCTGTG
    AAGCCAGACA AGATGGAGGA GACACTAACC TGTATCATCT GCCAGGACCT TCTGCACGAT
    TGTGTGAGTT TGCAGCCTTG TATGCACACA TTTTGTGCGG CTTGCTACTC TGGTTGGATG
    GAGCGTTCAT CTCTGTGCCC TACCTGCCGA TGTCCAGTGG AGCGGATTTG CAAAAACCAC
    ATCCTGAACA ACCTAGTGGA AGCATACCTT ATCCAGCACC CAGATAAAAG TCGCAGTGAA
    GAAGATGTGA GAAGTATGGA TGCAAGGAAT AAAATCACTC AAGATATGCT GCAACCCAAA
    GTCAGGAGGT CTTTCTCTGA TGAAGAGGGG AGTTCAGAGG ACCTGCTAGA GCTGTCTGAT
    GTCGACAGTG AATCCTCAGA TATCAGTCAG CCATACATTG TCTGCAGACA GTGTCCTGAA
    TACAGAAGGC AAGCGGTGCA GTCTCTTCCT TGCCCAGTCC CAGAGAGTGA GCTGGGAGCT
    ACACTGGCCC TTGGTGGGGA GGCACCTTCA ACATCTGCCA GCTTGCCAAC AGCAGCCCCG
    GATTACATGT GCCCTCTTCA AGGAAGCCAT GCCATATGCA CCTGCTGCTT CCAGCCTATG
    CCTGACCGGA GAGCTGAACG GGAGCAGGAT CCCCGCGTCG CCCCTCAGCA GTGTGCGGTG
    TGCCTGCAGC CCTTCTGCCA CCTGTACTGG GGCTGCACGA GGACTGGCTG CTTTGGCTGC
    TTGGCCCCAT TCTGTGAGCT CAACCTGGGG GACAAGTGCT TGGATGGAGT GCTGAACAAT
    AACAATTATG AATCGGACAT CCTGAAGAAT TACCTGGCAA CCAGGGGTCT GACATGGAAA
    AGTGTGTTGA CAGAGAGTCT CCTGGCTCTG CAGCGAGGTG TATTTATGCT GTCTGATTAC
    AGAATCACTG GAAATACTGT GCTGTGTTAC TGCTGTGGTC TGCGTAGCTT CCGAGAGCTG
    ACCTACCAGT ATCGTCAGAA CATTCCTGCT TCTGAGTTGC CAGTGACTGT AACATCCCGT
    CCTGACTGCT ACTGGGGCCG TAACTGTCGC ACTCAGGTGA AGGCTCACCA TGCAATGAAA
    TTCAATCACA TCTGTGAGCA AACAAGGTTC AAGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001276507.1
    CDS117..1895
    Translation

    Target ORF information:

    RefSeq Version NM_001289578.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001289578.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAGCTAC ACGGGGAAGA GCAGCCGCCG CCGCCGCAGG AACCCTGGGG GAGGCTTCTT 
    CGTCTAGGCG CAGAGGAGGA CGAACCCCAG ATCCTTCTTT GGAAACGCGA GTGGACCATC
    GGAAGGAGGA GAGGCTGTGA CCTCTCTTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC
    TGTAAACTTA CAGTGGATGA AATATCTGGT GAGGTGACAC TGGAAGACAC CAGCACCAAT
    GGAACAGTGA TCAATAAGCT GCAAGTTGTT AAGAAGCAGA CTTACCCTTT ACAGAGCGGG
    GATATCATCT ATTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT
    GAATCTTTAA GTGGCAAACA GAGCTTAACT CAAGACTCCC TTGGACCTGG GAACACAAGC
    ATCTCCCCAA AAGGACGCAG TTCACTTGTT GCAAATGGCG AACTCTCTAG CCTTTCTCCA
    GTTTTCCAAG ACAAAGAAGC ATCCTTTTCT TTGCTGGAAA GTAAAGACCA TGAGGAATTG
    GAGCCTGCCA AAAAAAAGAT GAAAGGAGAT GGGGAACTTG ACACGAACCT CCAGTTATTA
    GTTTCAGGCC AGCGTGGAAA TGCCCAAACC TCAAGTGAAG ATGTCAAAGA TGCCTCTGTG
    AAGCCAGACA AGATGGAGGA GACACTAACC TGTATCATCT GCCAGGACCT TCTGCACGAT
    TGTGTGAGTT TGCAGCCTTG TATGCACACA TTTTGTGCGG CTTGCTACTC TGGTTGGATG
    GAGCGTTCAT CTCTGTGCCC TACCTGCCGA TGTCCAGTGG AGCGGATTTG CAAAAACCAC
    ATCCTGAACA ACCTAGTGGA AGCATACCTT ATCCAGCACC CAGATAAAAG TCGCAGTGAA
    GAAGATGTGA GAAGTATGGA TGCAAGGAAT AAAATCACTC AAGATATGCT GCAACCCAAA
    GTCAGGAGGT CTTTCTCTGA TGAAGAGGGG AGTTCAGAGG ACCTGCTAGA GCTGTCTGAT
    GTCGACAGTG AATCCTCAGA TATCAGTCAG CCATACATTG TCTGCAGACA GTGTCCTGAA
    TACAGAAGGC AAGCGGTGCA GTCTCTTCCT TGCCCAGTCC CAGAGAGTGA GCTGGGAGCT
    ACACTGGCCC TTGGTGGGGA GGCACCTTCA ACATCTGCCA GCTTGCCAAC AGCAGCCCCG
    GATTACATGT GCCCTCTTCA AGGAAGCCAT GCCATATGCA CCTGCTGCTT CCAGCCTATG
    CCTGACCGGA GAGCTGAACG GGAGCAGGAT CCCCGCGTCG CCCCTCAGCA GTGTGCGGTG
    TGCCTGCAGC CCTTCTGCCA CCTGTACTGG GGCTGCACGA GGACTGGCTG CTTTGGCTGC
    TTGGCCCCAT TCTGTGAGCT CAACCTGGGG GACAAGTGCT TGGATGGAGT GCTGAACAAT
    AACAATTATG AATCGGACAT CCTGAAGAAT TACCTGGCAA CCAGGGGTCT GACATGGAAA
    AGTGTGTTGA CAGAGAGTCT CCTGGCTCTG CAGCGAGGTG TATTTATGCT GTCTGATTAC
    AGAATCACTG GAAATACTGT GCTGTGTTAC TGCTGTGGTC TGCGTAGCTT CCGAGAGCTG
    ACCTACCAGT ATCGTCAGAA CATTCCTGCT TCTGAGTTGC CAGTGACTGT AACATCCCGT
    CCTGACTGCT ACTGGGGCCG TAACTGTCGC ACTCAGGTGA AGGCTCACCA TGCAATGAAA
    TTCAATCACA TCTGTGAGCA AACAAGGTTC AAGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16462
    Clone ID Related Accession (Same CDS sequence) NM_001289580.1 , XM_030254419.1 , XM_030254418.1
    Accession Version NM_001289580.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1572bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase CHFR isoform 5
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC118260.7, AK048930.1 and AK169182.1. Transcript Variant: This variant (5) contains an alternate exon in the 5' region, uses an alternate in-frame splice site, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (5) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK048930.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
    CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA
    GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA
    AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA
    CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC
    TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA
    GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC
    CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA
    CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG
    CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC
    TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA
    TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA
    AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA
    GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC
    AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT
    CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA
    CCTTCAACAT CTGCCAGCTT GCCAACAGCC CCGGATTACA TGTGCCCTCT TCAAGGAAGC
    CATGCCATAT GCACCTGCTG CTTCCAGCCT ATGCCTGACC GGAGAGCTGA ACGGGAGCAG
    GATCCCCGCG TCGCCCCTCA GCAGTGTGCG GTGTGCCTGC AGCCCTTCTG CCACCTGTAC
    TGGGGCTGCA CGAGGACTGG CTGCTTTGGC TGCTTGGCCC CATTCTGTGA GCTCAACCTG
    GGGGACAAGT GCTTGGATGG AGTGCTGAAC AATAACAATT ATGAATCGGA CATCCTGAAG
    AATTACCTGG CAACCAGGGG TCTGACATGG AAAAGTGTGT TGACAGAGAG TCTCCTGGCT
    CTGCAGCGAG GTGTATTTAT GCTGTCTGAT TACAGAATCA CTGGAAATAC TGTGCTGTGT
    TACTGCTGTG GTCTGCGTAG CTTCCGAGAG CTGACCTACC AGTATCGTCA GAACATTCCT
    GCTTCTGAGT TGCCAGTGAC TGTAACATCC CGTCCTGACT GCTACTGGGG CCGTAACTGT
    CGCACTCAGG TGAAGGCTCA CCATGCAATG AAATTCAATC ACATCTGTGA GCAAACAAGG
    TTCAAGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001276509.1
    CDS707..2278
    Translation

    Target ORF information:

    RefSeq Version NM_001289580.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001289580.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
    CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA
    GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA
    AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA
    CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC
    TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA
    GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC
    CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA
    CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG
    CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC
    TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA
    TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA
    AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA
    GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC
    AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT
    CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA
    CCTTCAACAT CTGCCAGCTT GCCAACAGCC CCGGATTACA TGTGCCCTCT TCAAGGAAGC
    CATGCCATAT GCACCTGCTG CTTCCAGCCT ATGCCTGACC GGAGAGCTGA ACGGGAGCAG
    GATCCCCGCG TCGCCCCTCA GCAGTGTGCG GTGTGCCTGC AGCCCTTCTG CCACCTGTAC
    TGGGGCTGCA CGAGGACTGG CTGCTTTGGC TGCTTGGCCC CATTCTGTGA GCTCAACCTG
    GGGGACAAGT GCTTGGATGG AGTGCTGAAC AATAACAATT ATGAATCGGA CATCCTGAAG
    AATTACCTGG CAACCAGGGG TCTGACATGG AAAAGTGTGT TGACAGAGAG TCTCCTGGCT
    CTGCAGCGAG GTGTATTTAT GCTGTCTGAT TACAGAATCA CTGGAAATAC TGTGCTGTGT
    TACTGCTGTG GTCTGCGTAG CTTCCGAGAG CTGACCTACC AGTATCGTCA GAACATTCCT
    GCTTCTGAGT TGCCAGTGAC TGTAACATCC CGTCCTGACT GCTACTGGGG CCGTAACTGT
    CGCACTCAGG TGAAGGCTCA CCATGCAATG AAATTCAATC ACATCTGTGA GCAAACAAGG
    TTCAAGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16505
    Clone ID Related Accession (Same CDS sequence) NM_172717.4
    Accession Version NM_172717.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1992bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase CHFR isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC118260.7 and AK077629.1. On Jan 24, 2014 this sequence version replaced NM_172717.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK155295.1, AK155525.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCTAC ACGGGGAAGA GCAGCCGCCG CCGCCGCAGG AACCCTGGGG GAGGCTTCTT 
    CGTCTAGGCG CAGAGGAGGA CGAACCCCAG ATCCTTCTTT GGAAACGCGA GTGGACCATC
    GGAAGGAGGA GAGGCTGTGA CCTCTCTTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC
    TGTAAACTTA CAGTGGATGA AATATCTGGT GAGGTGACAC TGGAAGACAC CAGCACCAAT
    GGAACAGTGA TCAATAAGCT GCAAGTTGTT AAGAAGCAGA CTTACCCTTT ACAGAGCGGG
    GATATCATCT ATTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT
    GAATCTTTAA GTGGCAAACA GAGCTTAACT CAAGACTCCC TTGAAGCCAA TAAGGAAAAT
    ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA
    CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA
    GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA
    AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA
    CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC
    TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA
    GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC
    CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA
    CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG
    CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC
    TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA
    TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA
    AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA
    GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC
    AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT
    CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA
    CCTTCAACAT CTGCCAGCTT GCCAACAGCC CCGGATTACA TGTGCCCTCT TCAAGGAAGC
    CATGCCATAT GCACCTGCTG CTTCCAGCCT ATGCCTGACC GGAGAGCTGA ACGGGAGCAG
    GATCCCCGCG TCGCCCCTCA GCAGTGTGCG GTGTGCCTGC AGCCCTTCTG CCACCTGTAC
    TGGGGCTGCA CGAGGACTGG CTGCTTTGGC TGCTTGGCCC CATTCTGTGA GCTCAACCTG
    GGGGACAAGT GCTTGGATGG AGTGCTGAAC AATAACAATT ATGAATCGGA CATCCTGAAG
    AATTACCTGG CAACCAGGGG TCTGACATGG AAAAGTGTGT TGACAGAGAG TCTCCTGGCT
    CTGCAGCGAG GTGTATTTAT GCTGTCTGAT TACAGAATCA CTGGAAATAC TGTGCTGTGT
    TACTGCTGTG GTCTGCGTAG CTTCCGAGAG CTGACCTACC AGTATCGTCA GAACATTCCT
    GCTTCTGAGT TGCCAGTGAC TGTAACATCC CGTCCTGACT GCTACTGGGG CCGTAACTGT
    CGCACTCAGG TGAAGGCTCA CCATGCAATG AAATTCAATC ACATCTGTGA GCAAACAAGG
    TTCAAGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_766305.2
    CDS117..2108
    Translation

    Target ORF information:

    RefSeq Version NM_172717.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_172717.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCTAC ACGGGGAAGA GCAGCCGCCG CCGCCGCAGG AACCCTGGGG GAGGCTTCTT 
    CGTCTAGGCG CAGAGGAGGA CGAACCCCAG ATCCTTCTTT GGAAACGCGA GTGGACCATC
    GGAAGGAGGA GAGGCTGTGA CCTCTCTTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC
    TGTAAACTTA CAGTGGATGA AATATCTGGT GAGGTGACAC TGGAAGACAC CAGCACCAAT
    GGAACAGTGA TCAATAAGCT GCAAGTTGTT AAGAAGCAGA CTTACCCTTT ACAGAGCGGG
    GATATCATCT ATTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT
    GAATCTTTAA GTGGCAAACA GAGCTTAACT CAAGACTCCC TTGAAGCCAA TAAGGAAAAT
    ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA
    CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA
    GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA
    AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA
    CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC
    TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA
    GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC
    CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA
    CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG
    CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC
    TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA
    TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA
    AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA
    GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC
    AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT
    CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA
    CCTTCAACAT CTGCCAGCTT GCCAACAGCC CCGGATTACA TGTGCCCTCT TCAAGGAAGC
    CATGCCATAT GCACCTGCTG CTTCCAGCCT ATGCCTGACC GGAGAGCTGA ACGGGAGCAG
    GATCCCCGCG TCGCCCCTCA GCAGTGTGCG GTGTGCCTGC AGCCCTTCTG CCACCTGTAC
    TGGGGCTGCA CGAGGACTGG CTGCTTTGGC TGCTTGGCCC CATTCTGTGA GCTCAACCTG
    GGGGACAAGT GCTTGGATGG AGTGCTGAAC AATAACAATT ATGAATCGGA CATCCTGAAG
    AATTACCTGG CAACCAGGGG TCTGACATGG AAAAGTGTGT TGACAGAGAG TCTCCTGGCT
    CTGCAGCGAG GTGTATTTAT GCTGTCTGAT TACAGAATCA CTGGAAATAC TGTGCTGTGT
    TACTGCTGTG GTCTGCGTAG CTTCCGAGAG CTGACCTACC AGTATCGTCA GAACATTCCT
    GCTTCTGAGT TGCCAGTGAC TGTAACATCC CGTCCTGACT GCTACTGGGG CCGTAACTGT
    CGCACTCAGG TGAAGGCTCA CCATGCAATG AAATTCAATC ACATCTGTGA GCAAACAAGG
    TTCAAGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu17144
    Clone ID Related Accession (Same CDS sequence) NM_001289577.1
    Accession Version NM_001289577.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1995bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase CHFR isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC118260.7. On Jan 24, 2014 this sequence version replaced XM_006534914.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK154038.1, SRR1660811.16989.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCTAC ACGGGGAAGA GCAGCCGCCG CCGCCGCAGG AACCCTGGGG GAGGCTTCTT 
    CGTCTAGGCG CAGAGGAGGA CGAACCCCAG ATCCTTCTTT GGAAACGCGA GTGGACCATC
    GGAAGGAGGA GAGGCTGTGA CCTCTCTTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC
    TGTAAACTTA CAGTGGATGA AATATCTGGT GAGGTGACAC TGGAAGACAC CAGCACCAAT
    GGAACAGTGA TCAATAAGCT GCAAGTTGTT AAGAAGCAGA CTTACCCTTT ACAGAGCGGG
    GATATCATCT ATTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT
    GAATCTTTAA GTGGCAAACA GAGCTTAACT CAAGACTCCC TTGAAGCCAA TAAGGAAAAT
    ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA
    CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA
    GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA
    AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA
    CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC
    TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA
    GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC
    CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA
    CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG
    CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC
    TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA
    TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA
    AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA
    GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC
    AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT
    CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA
    CCTTCAACAT CTGCCAGCTT GCCAACAGCA GCCCCGGATT ACATGTGCCC TCTTCAAGGA
    AGCCATGCCA TATGCACCTG CTGCTTCCAG CCTATGCCTG ACCGGAGAGC TGAACGGGAG
    CAGGATCCCC GCGTCGCCCC TCAGCAGTGT GCGGTGTGCC TGCAGCCCTT CTGCCACCTG
    TACTGGGGCT GCACGAGGAC TGGCTGCTTT GGCTGCTTGG CCCCATTCTG TGAGCTCAAC
    CTGGGGGACA AGTGCTTGGA TGGAGTGCTG AACAATAACA ATTATGAATC GGACATCCTG
    AAGAATTACC TGGCAACCAG GGGTCTGACA TGGAAAAGTG TGTTGACAGA GAGTCTCCTG
    GCTCTGCAGC GAGGTGTATT TATGCTGTCT GATTACAGAA TCACTGGAAA TACTGTGCTG
    TGTTACTGCT GTGGTCTGCG TAGCTTCCGA GAGCTGACCT ACCAGTATCG TCAGAACATT
    CCTGCTTCTG AGTTGCCAGT GACTGTAACA TCCCGTCCTG ACTGCTACTG GGGCCGTAAC
    TGTCGCACTC AGGTGAAGGC TCACCATGCA ATGAAATTCA ATCACATCTG TGAGCAAACA
    AGGTTCAAGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001276506.1
    CDS117..2111
    Translation

    Target ORF information:

    RefSeq Version NM_001289577.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001289577.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCTAC ACGGGGAAGA GCAGCCGCCG CCGCCGCAGG AACCCTGGGG GAGGCTTCTT 
    CGTCTAGGCG CAGAGGAGGA CGAACCCCAG ATCCTTCTTT GGAAACGCGA GTGGACCATC
    GGAAGGAGGA GAGGCTGTGA CCTCTCTTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC
    TGTAAACTTA CAGTGGATGA AATATCTGGT GAGGTGACAC TGGAAGACAC CAGCACCAAT
    GGAACAGTGA TCAATAAGCT GCAAGTTGTT AAGAAGCAGA CTTACCCTTT ACAGAGCGGG
    GATATCATCT ATTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT
    GAATCTTTAA GTGGCAAACA GAGCTTAACT CAAGACTCCC TTGAAGCCAA TAAGGAAAAT
    ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA
    CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA
    GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA
    AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA
    CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC
    TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA
    GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC
    CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA
    CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG
    CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC
    TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA
    TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA
    AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA
    GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC
    AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT
    CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA
    CCTTCAACAT CTGCCAGCTT GCCAACAGCA GCCCCGGATT ACATGTGCCC TCTTCAAGGA
    AGCCATGCCA TATGCACCTG CTGCTTCCAG CCTATGCCTG ACCGGAGAGC TGAACGGGAG
    CAGGATCCCC GCGTCGCCCC TCAGCAGTGT GCGGTGTGCC TGCAGCCCTT CTGCCACCTG
    TACTGGGGCT GCACGAGGAC TGGCTGCTTT GGCTGCTTGG CCCCATTCTG TGAGCTCAAC
    CTGGGGGACA AGTGCTTGGA TGGAGTGCTG AACAATAACA ATTATGAATC GGACATCCTG
    AAGAATTACC TGGCAACCAG GGGTCTGACA TGGAAAAGTG TGTTGACAGA GAGTCTCCTG
    GCTCTGCAGC GAGGTGTATT TATGCTGTCT GATTACAGAA TCACTGGAAA TACTGTGCTG
    TGTTACTGCT GTGGTCTGCG TAGCTTCCGA GAGCTGACCT ACCAGTATCG TCAGAACATT
    CCTGCTTCTG AGTTGCCAGT GACTGTAACA TCCCGTCCTG ACTGCTACTG GGGCCGTAAC
    TGTCGCACTC AGGTGAAGGC TCACCATGCA ATGAAATTCA ATCACATCTG TGAGCAAACA
    AGGTTCAAGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16350
    Clone ID Related Accession (Same CDS sequence) XM_011249446.2 , XM_011249445.2 , NM_001289579.1
    Accession Version XM_011249446.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase CHFR isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249446.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
    CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA
    GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA
    AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA
    CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC
    TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA
    GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC
    CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA
    CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG
    CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC
    TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA
    TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA
    AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA
    GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC
    AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT
    CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA
    CCTTCAACAT CTGCCAGCTT GCCAACAGCA GCCCCGGATT ACATGTGCCC TCTTCAAGGA
    AGCCATGCCA TATGCACCTG CTGCTTCCAG CCTATGCCTG ACCGGAGAGC TGAACGGGAG
    CAGGATCCCC GCGTCGCCCC TCAGCAGTGT GCGGTGTGCC TGCAGCCCTT CTGCCACCTG
    TACTGGGGCT GCACGAGGAC TGGCTGCTTT GGCTGCTTGG CCCCATTCTG TGAGCTCAAC
    CTGGGGGACA AGTGCTTGGA TGGAGTGCTG AACAATAACA ATTATGAATC GGACATCCTG
    AAGAATTACC TGGCAACCAG GGGTCTGACA TGGAAAAGTG TGTTGACAGA GAGTCTCCTG
    GCTCTGCAGC GAGGTGTATT TATGCTGTCT GATTACAGAA TCACTGGAAA TACTGTGCTG
    TGTTACTGCT GTGGTCTGCG TAGCTTCCGA GAGCTGACCT ACCAGTATCG TCAGAACATT
    CCTGCTTCTG AGTTGCCAGT GACTGTAACA TCCCGTCCTG ACTGCTACTG GGGCCGTAAC
    TGTCGCACTC AGGTGAAGGC TCACCATGCA ATGAAATTCA ATCACATCTG TGAGCAAACA
    AGGTTCAAGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011247748.1
    CDS501..2075
    Translation

    Target ORF information:

    RefSeq Version XM_011249446.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011249446.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
    CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA
    GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA
    AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA
    CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC
    TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA
    GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC
    CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA
    CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG
    CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC
    TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA
    TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA
    AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA
    GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC
    AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT
    CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA
    CCTTCAACAT CTGCCAGCTT GCCAACAGCA GCCCCGGATT ACATGTGCCC TCTTCAAGGA
    AGCCATGCCA TATGCACCTG CTGCTTCCAG CCTATGCCTG ACCGGAGAGC TGAACGGGAG
    CAGGATCCCC GCGTCGCCCC TCAGCAGTGT GCGGTGTGCC TGCAGCCCTT CTGCCACCTG
    TACTGGGGCT GCACGAGGAC TGGCTGCTTT GGCTGCTTGG CCCCATTCTG TGAGCTCAAC
    CTGGGGGACA AGTGCTTGGA TGGAGTGCTG AACAATAACA ATTATGAATC GGACATCCTG
    AAGAATTACC TGGCAACCAG GGGTCTGACA TGGAAAAGTG TGTTGACAGA GAGTCTCCTG
    GCTCTGCAGC GAGGTGTATT TATGCTGTCT GATTACAGAA TCACTGGAAA TACTGTGCTG
    TGTTACTGCT GTGGTCTGCG TAGCTTCCGA GAGCTGACCT ACCAGTATCG TCAGAACATT
    CCTGCTTCTG AGTTGCCAGT GACTGTAACA TCCCGTCCTG ACTGCTACTG GGGCCGTAAC
    TGTCGCACTC AGGTGAAGGC TCACCATGCA ATGAAATTCA ATCACATCTG TGAGCAAACA
    AGGTTCAAGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16350
    Clone ID Related Accession (Same CDS sequence) XM_011249446.2 , XM_011249445.2 , NM_001289579.1
    Accession Version XM_011249445.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase CHFR isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249445.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
    CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA
    GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA
    AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA
    CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC
    TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA
    GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC
    CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA
    CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG
    CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC
    TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA
    TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA
    AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA
    GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC
    AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT
    CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA
    CCTTCAACAT CTGCCAGCTT GCCAACAGCA GCCCCGGATT ACATGTGCCC TCTTCAAGGA
    AGCCATGCCA TATGCACCTG CTGCTTCCAG CCTATGCCTG ACCGGAGAGC TGAACGGGAG
    CAGGATCCCC GCGTCGCCCC TCAGCAGTGT GCGGTGTGCC TGCAGCCCTT CTGCCACCTG
    TACTGGGGCT GCACGAGGAC TGGCTGCTTT GGCTGCTTGG CCCCATTCTG TGAGCTCAAC
    CTGGGGGACA AGTGCTTGGA TGGAGTGCTG AACAATAACA ATTATGAATC GGACATCCTG
    AAGAATTACC TGGCAACCAG GGGTCTGACA TGGAAAAGTG TGTTGACAGA GAGTCTCCTG
    GCTCTGCAGC GAGGTGTATT TATGCTGTCT GATTACAGAA TCACTGGAAA TACTGTGCTG
    TGTTACTGCT GTGGTCTGCG TAGCTTCCGA GAGCTGACCT ACCAGTATCG TCAGAACATT
    CCTGCTTCTG AGTTGCCAGT GACTGTAACA TCCCGTCCTG ACTGCTACTG GGGCCGTAAC
    TGTCGCACTC AGGTGAAGGC TCACCATGCA ATGAAATTCA ATCACATCTG TGAGCAAACA
    AGGTTCAAGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011247747.1
    CDS579..2153
    Translation

    Target ORF information:

    RefSeq Version XM_011249445.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011249445.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
    CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA
    GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA
    AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA
    CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC
    TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA
    GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC
    CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA
    CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG
    CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC
    TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA
    TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA
    AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA
    GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC
    AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT
    CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA
    CCTTCAACAT CTGCCAGCTT GCCAACAGCA GCCCCGGATT ACATGTGCCC TCTTCAAGGA
    AGCCATGCCA TATGCACCTG CTGCTTCCAG CCTATGCCTG ACCGGAGAGC TGAACGGGAG
    CAGGATCCCC GCGTCGCCCC TCAGCAGTGT GCGGTGTGCC TGCAGCCCTT CTGCCACCTG
    TACTGGGGCT GCACGAGGAC TGGCTGCTTT GGCTGCTTGG CCCCATTCTG TGAGCTCAAC
    CTGGGGGACA AGTGCTTGGA TGGAGTGCTG AACAATAACA ATTATGAATC GGACATCCTG
    AAGAATTACC TGGCAACCAG GGGTCTGACA TGGAAAAGTG TGTTGACAGA GAGTCTCCTG
    GCTCTGCAGC GAGGTGTATT TATGCTGTCT GATTACAGAA TCACTGGAAA TACTGTGCTG
    TGTTACTGCT GTGGTCTGCG TAGCTTCCGA GAGCTGACCT ACCAGTATCG TCAGAACATT
    CCTGCTTCTG AGTTGCCAGT GACTGTAACA TCCCGTCCTG ACTGCTACTG GGGCCGTAAC
    TGTCGCACTC AGGTGAAGGC TCACCATGCA ATGAAATTCA ATCACATCTG TGAGCAAACA
    AGGTTCAAGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35453
    Clone ID Related Accession (Same CDS sequence) XM_006534915.1
    Accession Version XM_006534915.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1854bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase CHFR isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAGATCTC CCAGCAATAA ACTGGTCTCT GGAGATCACT GTAAACTTAC AGTGGATGAA 
    ATATCTGGTG AGGTGACACT GGAAGACACC AGCACCAATG GAACAGTGAT CAATAAGCTG
    CAAGTTGTTA AGAAGCAGAC TTACCCTTTA CAGAGCGGGG ATATCATCTA TTTGGTGTAC
    AGGAAGAATG AGCCAGAACA CAATGTGGCA TACCTCTATG AATCTTTAAG TGGCAAACAG
    AGCTTAACTC AAGACTCCCT TGAAGCCAAT AAGGAAAATA TGTTCCATGT GACCAAAGAT
    TGCTCAGGTC CAGGGCAGGG TGATGATCCC CAGGTTCCAC TATTGTCACC CATGGCTCAG
    ACATGCTTAG AGGAACCACA GCCATCAACA TCGACATCAG ACCTCCTCCC CACGGCCTCT
    ACCTCTTCTA CGGAGCCAGA GCTGACCTCT GCAGGGCAAA AGCATTCTTC TAGCTCTGGA
    CCTGGGAACA CAAGCATCTC CCCAAAAGGA CGCAGTTCAC TTGTTGCAAA TGGCGAACTC
    TCTAGCCTTT CTCCAGTTTT CCAAGACAAA GAAGCATCCT TTTCTTTGCT GGAAAGTAAA
    GACCATGAGG AATTGGAGCC TGCCAAAAAA AAGATGAAAG GAGATGGGGA ACTTGACACG
    AACCTCCAGT TATTAGTTTC AGGCCAGCGT GGAAATGCCC AAACCTCAAG TGAAGATGTC
    AAAGATGCCT CTGTGAAGCC AGACAAGATG GAGGAGACAC TAACCTGTAT CATCTGCCAG
    GACCTTCTGC ACGATTGTGT GAGTTTGCAG CCTTGTATGC ACACATTTTG TGCGGCTTGC
    TACTCTGGTT GGATGGAGCG TTCATCTCTG TGCCCTACCT GCCGATGTCC AGTGGAGCGG
    ATTTGCAAAA ACCACATCCT GAACAACCTA GTGGAAGCAT ACCTTATCCA GCACCCAGAT
    AAAAGTCGCA GTGAAGAAGA TGTGAGAAGT ATGGATGCAA GGAATAAAAT CACTCAAGAT
    ATGCTGCAAC CCAAAGTCAG GAGGTCTTTC TCTGATGAAG AGGGGAGTTC AGAGGACCTG
    CTAGAGCTGT CTGATGTCGA CAGTGAATCC TCAGATATCA GTCAGCCATA CATTGTCTGC
    AGACAGTGTC CTGAATACAG AAGGCAAGCG GTGCAGTCTC TTCCTTGCCC AGTCCCAGAG
    AGTGAGCTGG GAGCTACACT GGCCCTTGGT GGGGAGGCAC CTTCAACATC TGCCAGCTTG
    CCAACAGCAG CCCCGGATTA CATGTGCCCT CTTCAAGGAA GCCATGCCAT ATGCACCTGC
    TGCTTCCAGC CTATGCCTGA CCGGAGAGCT GAACGGGAGC AGGATCCCCG CGTCGCCCCT
    CAGCAGTGTG CGGTGTGCCT GCAGCCCTTC TGCCACCTGT ACTGGGGCTG CACGAGGACT
    GGCTGCTTTG GCTGCTTGGC CCCATTCTGT GAGCTCAACC TGGGGGACAA GTGCTTGGAT
    GGAGTGCTGA ACAATAACAA TTATGAATCG GACATCCTGA AGAATTACCT GGCAACCAGG
    GGTCTGACAT GGAAAAGTGT GTTGACAGAG AGTCTCCTGG CTCTGCAGCG AGGTGTATTT
    ATGCTGTCTG ATTACAGAAT CACTGGAAAT ACTGTGCTGT GTTACTGCTG TGGTCTGCGT
    AGCTTCCGAG AGCTGACCTA CCAGTATCGT CAGAACATTC CTGCTTCTGA GTTGCCAGTG
    ACTGTAACAT CCCGTCCTGA CTGCTACTGG GGCCGTAACT GTCGCACTCA GGTGAAGGCT
    CACCATGCAA TGAAATTCAA TCACATCTGT GAGCAAACAA GGTTCAAGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006534978.1
    CDS150..2003
    Translation

    Target ORF information:

    RefSeq Version XM_006534915.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006534915.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAGATCTC CCAGCAATAA ACTGGTCTCT GGAGATCACT GTAAACTTAC AGTGGATGAA 
    ATATCTGGTG AGGTGACACT GGAAGACACC AGCACCAATG GAACAGTGAT CAATAAGCTG
    CAAGTTGTTA AGAAGCAGAC TTACCCTTTA CAGAGCGGGG ATATCATCTA TTTGGTGTAC
    AGGAAGAATG AGCCAGAACA CAATGTGGCA TACCTCTATG AATCTTTAAG TGGCAAACAG
    AGCTTAACTC AAGACTCCCT TGAAGCCAAT AAGGAAAATA TGTTCCATGT GACCAAAGAT
    TGCTCAGGTC CAGGGCAGGG TGATGATCCC CAGGTTCCAC TATTGTCACC CATGGCTCAG
    ACATGCTTAG AGGAACCACA GCCATCAACA TCGACATCAG ACCTCCTCCC CACGGCCTCT
    ACCTCTTCTA CGGAGCCAGA GCTGACCTCT GCAGGGCAAA AGCATTCTTC TAGCTCTGGA
    CCTGGGAACA CAAGCATCTC CCCAAAAGGA CGCAGTTCAC TTGTTGCAAA TGGCGAACTC
    TCTAGCCTTT CTCCAGTTTT CCAAGACAAA GAAGCATCCT TTTCTTTGCT GGAAAGTAAA
    GACCATGAGG AATTGGAGCC TGCCAAAAAA AAGATGAAAG GAGATGGGGA ACTTGACACG
    AACCTCCAGT TATTAGTTTC AGGCCAGCGT GGAAATGCCC AAACCTCAAG TGAAGATGTC
    AAAGATGCCT CTGTGAAGCC AGACAAGATG GAGGAGACAC TAACCTGTAT CATCTGCCAG
    GACCTTCTGC ACGATTGTGT GAGTTTGCAG CCTTGTATGC ACACATTTTG TGCGGCTTGC
    TACTCTGGTT GGATGGAGCG TTCATCTCTG TGCCCTACCT GCCGATGTCC AGTGGAGCGG
    ATTTGCAAAA ACCACATCCT GAACAACCTA GTGGAAGCAT ACCTTATCCA GCACCCAGAT
    AAAAGTCGCA GTGAAGAAGA TGTGAGAAGT ATGGATGCAA GGAATAAAAT CACTCAAGAT
    ATGCTGCAAC CCAAAGTCAG GAGGTCTTTC TCTGATGAAG AGGGGAGTTC AGAGGACCTG
    CTAGAGCTGT CTGATGTCGA CAGTGAATCC TCAGATATCA GTCAGCCATA CATTGTCTGC
    AGACAGTGTC CTGAATACAG AAGGCAAGCG GTGCAGTCTC TTCCTTGCCC AGTCCCAGAG
    AGTGAGCTGG GAGCTACACT GGCCCTTGGT GGGGAGGCAC CTTCAACATC TGCCAGCTTG
    CCAACAGCAG CCCCGGATTA CATGTGCCCT CTTCAAGGAA GCCATGCCAT ATGCACCTGC
    TGCTTCCAGC CTATGCCTGA CCGGAGAGCT GAACGGGAGC AGGATCCCCG CGTCGCCCCT
    CAGCAGTGTG CGGTGTGCCT GCAGCCCTTC TGCCACCTGT ACTGGGGCTG CACGAGGACT
    GGCTGCTTTG GCTGCTTGGC CCCATTCTGT GAGCTCAACC TGGGGGACAA GTGCTTGGAT
    GGAGTGCTGA ACAATAACAA TTATGAATCG GACATCCTGA AGAATTACCT GGCAACCAGG
    GGTCTGACAT GGAAAAGTGT GTTGACAGAG AGTCTCCTGG CTCTGCAGCG AGGTGTATTT
    ATGCTGTCTG ATTACAGAAT CACTGGAAAT ACTGTGCTGT GTTACTGCTG TGGTCTGCGT
    AGCTTCCGAG AGCTGACCTA CCAGTATCGT CAGAACATTC CTGCTTCTGA GTTGCCAGTG
    ACTGTAACAT CCCGTCCTGA CTGCTACTGG GGCCGTAACT GTCGCACTCA GGTGAAGGCT
    CACCATGCAA TGAAATTCAA TCACATCTGT GAGCAAACAA GGTTCAAGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu88879
    Clone ID Related Accession (Same CDS sequence) XM_030254420.1
    Accession Version XM_030254420.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1107bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase CHFR isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGAGGAGA CACTAACCTG TATCATCTGC CAGGACCTTC TGCACGATTG TGTGAGTTTG 
    CAGCCTTGTA TGCACACATT TTGTGCGGCT TGCTACTCTG GTTGGATGGA GCGTTCATCT
    CTGTGCCCTA CCTGCCGATG TCCAGTGGAG CGGATTTGCA AAAACCACAT CCTGAACAAC
    CTAGTGGAAG CATACCTTAT CCAGCACCCA GATAAAAGTC GCAGTGAAGA AGATGTGAGA
    AGTATGGATG CAAGGAATAA AATCACTCAA GATATGCTGC AACCCAAAGT CAGGAGGTCT
    TTCTCTGATG AAGAGGGGAG TTCAGAGGAC CTGCTAGAGC TGTCTGATGT CGACAGTGAA
    TCCTCAGATA TCAGTCAGCC ATACATTGTC TGCAGACAGT GTCCTGAATA CAGAAGGCAA
    GCGGTGCAGT CTCTTCCTTG CCCAGTCCCA GAGAGTGAGC TGGGAGCTAC ACTGGCCCTT
    GGTGGGGAGG CACCTTCAAC ATCTGCCAGC TTGCCAACAG CAGCCCCGGA TTACATGTGC
    CCTCTTCAAG GAAGCCATGC CATATGCACC TGCTGCTTCC AGCCTATGCC TGACCGGAGA
    GCTGAACGGG AGCAGGATCC CCGCGTCGCC CCTCAGCAGT GTGCGGTGTG CCTGCAGCCC
    TTCTGCCACC TGTACTGGGG CTGCACGAGG ACTGGCTGCT TTGGCTGCTT GGCCCCATTC
    TGTGAGCTCA ACCTGGGGGA CAAGTGCTTG GATGGAGTGC TGAACAATAA CAATTATGAA
    TCGGACATCC TGAAGAATTA CCTGGCAACC AGGGGTCTGA CATGGAAAAG TGTGTTGACA
    GAGAGTCTCC TGGCTCTGCA GCGAGGTGTA TTTATGCTGT CTGATTACAG AATCACTGGA
    AATACTGTGC TGTGTTACTG CTGTGGTCTG CGTAGCTTCC GAGAGCTGAC CTACCAGTAT
    CGTCAGAACA TTCCTGCTTC TGAGTTGCCA GTGACTGTAA CATCCCGTCC TGACTGCTAC
    TGGGGCCGTA ACTGTCGCAC TCAGGTGAAG GCTCACCATG CAATGAAATT CAATCACATC
    TGTGAGCAAA CAAGGTTCAA GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030110280.1
    CDS1176..2282
    Translation

    Target ORF information:

    RefSeq Version XM_030254420.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030254420.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGAGGAGA CACTAACCTG TATCATCTGC CAGGACCTTC TGCACGATTG TGTGAGTTTG 
    CAGCCTTGTA TGCACACATT TTGTGCGGCT TGCTACTCTG GTTGGATGGA GCGTTCATCT
    CTGTGCCCTA CCTGCCGATG TCCAGTGGAG CGGATTTGCA AAAACCACAT CCTGAACAAC
    CTAGTGGAAG CATACCTTAT CCAGCACCCA GATAAAAGTC GCAGTGAAGA AGATGTGAGA
    AGTATGGATG CAAGGAATAA AATCACTCAA GATATGCTGC AACCCAAAGT CAGGAGGTCT
    TTCTCTGATG AAGAGGGGAG TTCAGAGGAC CTGCTAGAGC TGTCTGATGT CGACAGTGAA
    TCCTCAGATA TCAGTCAGCC ATACATTGTC TGCAGACAGT GTCCTGAATA CAGAAGGCAA
    GCGGTGCAGT CTCTTCCTTG CCCAGTCCCA GAGAGTGAGC TGGGAGCTAC ACTGGCCCTT
    GGTGGGGAGG CACCTTCAAC ATCTGCCAGC TTGCCAACAG CAGCCCCGGA TTACATGTGC
    CCTCTTCAAG GAAGCCATGC CATATGCACC TGCTGCTTCC AGCCTATGCC TGACCGGAGA
    GCTGAACGGG AGCAGGATCC CCGCGTCGCC CCTCAGCAGT GTGCGGTGTG CCTGCAGCCC
    TTCTGCCACC TGTACTGGGG CTGCACGAGG ACTGGCTGCT TTGGCTGCTT GGCCCCATTC
    TGTGAGCTCA ACCTGGGGGA CAAGTGCTTG GATGGAGTGC TGAACAATAA CAATTATGAA
    TCGGACATCC TGAAGAATTA CCTGGCAACC AGGGGTCTGA CATGGAAAAG TGTGTTGACA
    GAGAGTCTCC TGGCTCTGCA GCGAGGTGTA TTTATGCTGT CTGATTACAG AATCACTGGA
    AATACTGTGC TGTGTTACTG CTGTGGTCTG CGTAGCTTCC GAGAGCTGAC CTACCAGTAT
    CGTCAGAACA TTCCTGCTTC TGAGTTGCCA GTGACTGTAA CATCCCGTCC TGACTGCTAC
    TGGGGCCGTA ACTGTCGCAC TCAGGTGAAG GCTCACCATG CAATGAAATT CAATCACATC
    TGTGAGCAAA CAAGGTTCAA GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu88880
    Clone ID Related Accession (Same CDS sequence) XM_030254421.1
    Accession Version XM_030254421.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 861bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase CHFR isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGATGCAA GGAATAAAAT CACTCAAGAT ATGCTGCAAC CCAAAGTCAG GAGGTCTTTC 
    TCTGATGAAG AGGGGAGTTC AGAGGACCTG CTAGAGCTGT CTGATGTCGA CAGTGAATCC
    TCAGATATCA GTCAGCCATA CATTGTCTGC AGACAGTGTC CTGAATACAG AAGGCAAGCG
    GTGCAGTCTC TTCCTTGCCC AGTCCCAGAG AGTGAGCTGG GAGCTACACT GGCCCTTGGT
    GGGGAGGCAC CTTCAACATC TGCCAGCTTG CCAACAGCCC CGGATTACAT GTGCCCTCTT
    CAAGGAAGCC ATGCCATATG CACCTGCTGC TTCCAGCCTA TGCCTGACCG GAGAGCTGAA
    CGGGAGCAGG ATCCCCGCGT CGCCCCTCAG CAGTGTGCGG TGTGCCTGCA GCCCTTCTGC
    CACCTGTACT GGGGCTGCAC GAGGACTGGC TGCTTTGGCT GCTTGGCCCC ATTCTGTGAG
    CTCAACCTGG GGGACAAGTG CTTGGATGGA GTGCTGAACA ATAACAATTA TGAATCGGAC
    ATCCTGAAGA ATTACCTGGC AACCAGGGGT CTGACATGGA AAAGTGTGTT GACAGAGAGT
    CTCCTGGCTC TGCAGCGAGG TGTATTTATG CTGTCTGATT ACAGAATCAC TGGAAATACT
    GTGCTGTGTT ACTGCTGTGG TCTGCGTAGC TTCCGAGAGC TGACCTACCA GTATCGTCAG
    AACATTCCTG CTTCTGAGTT GCCAGTGACT GTAACATCCC GTCCTGACTG CTACTGGGGC
    CGTAACTGTC GCACTCAGGT GAAGGCTCAC CATGCAATGA AATTCAATCA CATCTGTGAG
    CAAACAAGGT TCAAGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030110281.1
    CDS1554..2414
    Translation

    Target ORF information:

    RefSeq Version XM_030254421.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030254421.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGATGCAA GGAATAAAAT CACTCAAGAT ATGCTGCAAC CCAAAGTCAG GAGGTCTTTC 
    TCTGATGAAG AGGGGAGTTC AGAGGACCTG CTAGAGCTGT CTGATGTCGA CAGTGAATCC
    TCAGATATCA GTCAGCCATA CATTGTCTGC AGACAGTGTC CTGAATACAG AAGGCAAGCG
    GTGCAGTCTC TTCCTTGCCC AGTCCCAGAG AGTGAGCTGG GAGCTACACT GGCCCTTGGT
    GGGGAGGCAC CTTCAACATC TGCCAGCTTG CCAACAGCCC CGGATTACAT GTGCCCTCTT
    CAAGGAAGCC ATGCCATATG CACCTGCTGC TTCCAGCCTA TGCCTGACCG GAGAGCTGAA
    CGGGAGCAGG ATCCCCGCGT CGCCCCTCAG CAGTGTGCGG TGTGCCTGCA GCCCTTCTGC
    CACCTGTACT GGGGCTGCAC GAGGACTGGC TGCTTTGGCT GCTTGGCCCC ATTCTGTGAG
    CTCAACCTGG GGGACAAGTG CTTGGATGGA GTGCTGAACA ATAACAATTA TGAATCGGAC
    ATCCTGAAGA ATTACCTGGC AACCAGGGGT CTGACATGGA AAAGTGTGTT GACAGAGAGT
    CTCCTGGCTC TGCAGCGAGG TGTATTTATG CTGTCTGATT ACAGAATCAC TGGAAATACT
    GTGCTGTGTT ACTGCTGTGG TCTGCGTAGC TTCCGAGAGC TGACCTACCA GTATCGTCAG
    AACATTCCTG CTTCTGAGTT GCCAGTGACT GTAACATCCC GTCCTGACTG CTACTGGGGC
    CGTAACTGTC GCACTCAGGT GAAGGCTCAC CATGCAATGA AATTCAATCA CATCTGTGAG
    CAAACAAGGT TCAAGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16462
    Clone ID Related Accession (Same CDS sequence) NM_001289580.1 , XM_030254419.1 , XM_030254418.1
    Accession Version XM_030254419.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1572bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase CHFR isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
    CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA
    GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA
    AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA
    CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC
    TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA
    GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC
    CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA
    CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG
    CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC
    TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA
    TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA
    AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA
    GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC
    AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT
    CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA
    CCTTCAACAT CTGCCAGCTT GCCAACAGCC CCGGATTACA TGTGCCCTCT TCAAGGAAGC
    CATGCCATAT GCACCTGCTG CTTCCAGCCT ATGCCTGACC GGAGAGCTGA ACGGGAGCAG
    GATCCCCGCG TCGCCCCTCA GCAGTGTGCG GTGTGCCTGC AGCCCTTCTG CCACCTGTAC
    TGGGGCTGCA CGAGGACTGG CTGCTTTGGC TGCTTGGCCC CATTCTGTGA GCTCAACCTG
    GGGGACAAGT GCTTGGATGG AGTGCTGAAC AATAACAATT ATGAATCGGA CATCCTGAAG
    AATTACCTGG CAACCAGGGG TCTGACATGG AAAAGTGTGT TGACAGAGAG TCTCCTGGCT
    CTGCAGCGAG GTGTATTTAT GCTGTCTGAT TACAGAATCA CTGGAAATAC TGTGCTGTGT
    TACTGCTGTG GTCTGCGTAG CTTCCGAGAG CTGACCTACC AGTATCGTCA GAACATTCCT
    GCTTCTGAGT TGCCAGTGAC TGTAACATCC CGTCCTGACT GCTACTGGGG CCGTAACTGT
    CGCACTCAGG TGAAGGCTCA CCATGCAATG AAATTCAATC ACATCTGTGA GCAAACAAGG
    TTCAAGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030110279.1
    CDS412..1983
    Translation

    Target ORF information:

    RefSeq Version XM_030254419.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030254419.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
    CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA
    GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA
    AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA
    CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC
    TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA
    GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC
    CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA
    CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG
    CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC
    TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA
    TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA
    AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA
    GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC
    AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT
    CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA
    CCTTCAACAT CTGCCAGCTT GCCAACAGCC CCGGATTACA TGTGCCCTCT TCAAGGAAGC
    CATGCCATAT GCACCTGCTG CTTCCAGCCT ATGCCTGACC GGAGAGCTGA ACGGGAGCAG
    GATCCCCGCG TCGCCCCTCA GCAGTGTGCG GTGTGCCTGC AGCCCTTCTG CCACCTGTAC
    TGGGGCTGCA CGAGGACTGG CTGCTTTGGC TGCTTGGCCC CATTCTGTGA GCTCAACCTG
    GGGGACAAGT GCTTGGATGG AGTGCTGAAC AATAACAATT ATGAATCGGA CATCCTGAAG
    AATTACCTGG CAACCAGGGG TCTGACATGG AAAAGTGTGT TGACAGAGAG TCTCCTGGCT
    CTGCAGCGAG GTGTATTTAT GCTGTCTGAT TACAGAATCA CTGGAAATAC TGTGCTGTGT
    TACTGCTGTG GTCTGCGTAG CTTCCGAGAG CTGACCTACC AGTATCGTCA GAACATTCCT
    GCTTCTGAGT TGCCAGTGAC TGTAACATCC CGTCCTGACT GCTACTGGGG CCGTAACTGT
    CGCACTCAGG TGAAGGCTCA CCATGCAATG AAATTCAATC ACATCTGTGA GCAAACAAGG
    TTCAAGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16462
    Clone ID Related Accession (Same CDS sequence) NM_001289580.1 , XM_030254419.1 , XM_030254418.1
    Accession Version XM_030254418.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1572bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase CHFR isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
    CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA
    GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA
    AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA
    CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC
    TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA
    GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC
    CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA
    CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG
    CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC
    TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA
    TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA
    AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA
    GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC
    AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT
    CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA
    CCTTCAACAT CTGCCAGCTT GCCAACAGCC CCGGATTACA TGTGCCCTCT TCAAGGAAGC
    CATGCCATAT GCACCTGCTG CTTCCAGCCT ATGCCTGACC GGAGAGCTGA ACGGGAGCAG
    GATCCCCGCG TCGCCCCTCA GCAGTGTGCG GTGTGCCTGC AGCCCTTCTG CCACCTGTAC
    TGGGGCTGCA CGAGGACTGG CTGCTTTGGC TGCTTGGCCC CATTCTGTGA GCTCAACCTG
    GGGGACAAGT GCTTGGATGG AGTGCTGAAC AATAACAATT ATGAATCGGA CATCCTGAAG
    AATTACCTGG CAACCAGGGG TCTGACATGG AAAAGTGTGT TGACAGAGAG TCTCCTGGCT
    CTGCAGCGAG GTGTATTTAT GCTGTCTGAT TACAGAATCA CTGGAAATAC TGTGCTGTGT
    TACTGCTGTG GTCTGCGTAG CTTCCGAGAG CTGACCTACC AGTATCGTCA GAACATTCCT
    GCTTCTGAGT TGCCAGTGAC TGTAACATCC CGTCCTGACT GCTACTGGGG CCGTAACTGT
    CGCACTCAGG TGAAGGCTCA CCATGCAATG AAATTCAATC ACATCTGTGA GCAAACAAGG
    TTCAAGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030110278.1
    CDS580..2151
    Translation

    Target ORF information:

    RefSeq Version XM_030254418.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030254418.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
    CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA
    GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA
    AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA
    CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC
    TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA
    GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC
    CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA
    CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG
    CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC
    TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA
    TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA
    AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA
    GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC
    AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT
    CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA
    CCTTCAACAT CTGCCAGCTT GCCAACAGCC CCGGATTACA TGTGCCCTCT TCAAGGAAGC
    CATGCCATAT GCACCTGCTG CTTCCAGCCT ATGCCTGACC GGAGAGCTGA ACGGGAGCAG
    GATCCCCGCG TCGCCCCTCA GCAGTGTGCG GTGTGCCTGC AGCCCTTCTG CCACCTGTAC
    TGGGGCTGCA CGAGGACTGG CTGCTTTGGC TGCTTGGCCC CATTCTGTGA GCTCAACCTG
    GGGGACAAGT GCTTGGATGG AGTGCTGAAC AATAACAATT ATGAATCGGA CATCCTGAAG
    AATTACCTGG CAACCAGGGG TCTGACATGG AAAAGTGTGT TGACAGAGAG TCTCCTGGCT
    CTGCAGCGAG GTGTATTTAT GCTGTCTGAT TACAGAATCA CTGGAAATAC TGTGCTGTGT
    TACTGCTGTG GTCTGCGTAG CTTCCGAGAG CTGACCTACC AGTATCGTCA GAACATTCCT
    GCTTCTGAGT TGCCAGTGAC TGTAACATCC CGTCCTGACT GCTACTGGGG CCGTAACTGT
    CGCACTCAGG TGAAGGCTCA CCATGCAATG AAATTCAATC ACATCTGTGA GCAAACAAGG
    TTCAAGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.