Cdc42bpa cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cdc42bpa
Entrez Gene ID	226751
Full Name	CDC42 binding protein kinase alpha
Synonyms	A930014J19Rik,DMPK-like
General protein information	Preferred Names CDC42 binding protein kinase alpha Names serine/threonine-protein kinase MRCK alpha myotonic dystrophy kinase-related CDC-42-binding kinase-alpha
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 H4

mRNA and Protein(s)

mRNA	Protein	Name
XM_006496804.3	XP_006496867.1	serine/threonine-protein kinase MRCK alpha isoform X4
XM_017320130.2	XP_017175619.1	serine/threonine-protein kinase MRCK alpha isoform X10
XM_017320148.1	XP_017175637.1	serine/threonine-protein kinase MRCK alpha isoform X14
NM_001359545.1	NP_001346474.1	serine/threonine-protein kinase MRCK alpha isoform 8
NM_001346805.1	NP_001333734.1	serine/threonine-protein kinase MRCK alpha isoform 3
NM_001359541.1	NP_001346470.1	serine/threonine-protein kinase MRCK alpha isoform 4
NM_001359549.1	NP_001346478.1	serine/threonine-protein kinase MRCK alpha isoform 12
XM_006496802.3	XP_006496865.1	serine/threonine-protein kinase MRCK alpha isoform X2
XM_006496809.3	XP_006496872.1	serine/threonine-protein kinase MRCK alpha isoform X11
XM_017320129.1	XP_017175618.1	serine/threonine-protein kinase MRCK alpha isoform X13
XM_017320145.1	XP_017175634.1	serine/threonine-protein kinase MRCK alpha isoform X22
XM_006496808.3	XP_006496871.1	serine/threonine-protein kinase MRCK alpha isoform X8
XM_017320131.1	XP_017175620.1	serine/threonine-protein kinase MRCK alpha isoform X15
XM_017320134.1	XP_017175623.1	serine/threonine-protein kinase MRCK alpha isoform X18
XM_017320142.1	XP_017175631.1	serine/threonine-protein kinase MRCK alpha isoform X20
XM_017320119.2	XP_017175608.1	serine/threonine-protein kinase MRCK alpha isoform X1
XM_006496803.4	XP_006496866.1	serine/threonine-protein kinase MRCK alpha isoform X3
XM_006496806.4	XP_006496869.1	serine/threonine-protein kinase MRCK alpha isoform X5
XM_017320129.2	XP_017175618.1	serine/threonine-protein kinase MRCK alpha isoform X9
XM_011238803.3	XP_011237105.1	serine/threonine-protein kinase MRCK alpha isoform X10
XM_030253509.1	XP_030109369.1	serine/threonine-protein kinase MRCK alpha isoform X11
XM_011238803.2	XP_011237105.1	serine/threonine-protein kinase MRCK alpha isoform X14
XM_017320127.1	XP_017175616.1	serine/threonine-protein kinase MRCK alpha isoform X10
XM_017320146.1	XP_017175635.1	serine/threonine-protein kinase MRCK alpha isoform X23
XM_006496802.4	XP_006496865.1	serine/threonine-protein kinase MRCK alpha isoform X2
XM_006496804.4	XP_006496867.1	serine/threonine-protein kinase MRCK alpha isoform X4
XM_030253510.1	XP_030109370.1	serine/threonine-protein kinase MRCK alpha isoform X12
XM_011238804.3	XP_011237106.1	serine/threonine-protein kinase MRCK alpha isoform X13
NM_001346804.1	NP_001333733.1	serine/threonine-protein kinase MRCK alpha isoform 2
NM_001359548.1	NP_001346477.1	serine/threonine-protein kinase MRCK alpha isoform 11
NM_001359547.1	NP_001346476.1	serine/threonine-protein kinase MRCK alpha isoform 10
NM_001359542.1	NP_001346471.1	serine/threonine-protein kinase MRCK alpha isoform 5
XM_006496812.3	XP_006496875.1	serine/threonine-protein kinase MRCK alpha isoform X16
XM_006496803.3	XP_006496866.1	serine/threonine-protein kinase MRCK alpha isoform X3
XM_006496807.3	XP_006496870.1	serine/threonine-protein kinase MRCK alpha isoform X7
XM_017320130.1	XP_017175619.1	serine/threonine-protein kinase MRCK alpha isoform X14
XM_011238804.2	XP_011237106.1	serine/threonine-protein kinase MRCK alpha isoform X21
XM_006496809.4	XP_006496872.1	serine/threonine-protein kinase MRCK alpha isoform X8
NM_001359543.1	NP_001346472.1	serine/threonine-protein kinase MRCK alpha isoform 6
NM_001359544.1	NP_001346473.1	serine/threonine-protein kinase MRCK alpha isoform 7
NM_001033285.1	NP_001028457.1	serine/threonine-protein kinase MRCK alpha isoform 1
XM_017320119.1	XP_017175608.1	serine/threonine-protein kinase MRCK alpha isoform X1
XM_006496801.3	XP_006496864.1	serine/threonine-protein kinase MRCK alpha isoform X1
XM_006496806.3	XP_006496869.1	serine/threonine-protein kinase MRCK alpha isoform X6
XM_006496805.3	XP_006496868.1	serine/threonine-protein kinase MRCK alpha isoform X5
XM_017320132.1	XP_017175621.1	serine/threonine-protein kinase MRCK alpha isoform X17
XM_006496807.4	XP_006496870.1	serine/threonine-protein kinase MRCK alpha isoform X6
XM_011238802.2	XP_011237104.1	serine/threonine-protein kinase MRCK alpha isoform X7
NM_001359546.1	NP_001346475.1	serine/threonine-protein kinase MRCK alpha isoform 9

Pathways from BioSystems

Homologies

Homo sapiens (human)	CDC42BPA	NP_003598.2
	CDC42BPA	XP_002801950.1
Canis lupus familiaris (dog)	CDC42BPA	XP_005622674.1
Gallus gallus (chicken)	CDC42BPA	NP_001186372.1
Xenopus tropicalis (tropical clawed frog)	cdc42bpa	XP_002938889.2
Pan troglodytes (chimpanzee)	CDC42BPA	XP_003949757.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012090	XP_320436.4
Rattus norvegicus (Norway rat)	Cdc42bpa	NP_446109.1
Danio rerio (zebrafish)	si:ch211-89p3.3	NP_001038559.1
Drosophila melanogaster (fruit fly)	gek	NP_523837.2
Caenorhabditis elegans (roundworm)	mrck-1	NP_504599.2
Mus musculus (house mouse)	Cdc42bpa	NP_001028457.1

Gene Ontology
Bibliography

Related articles in PubMed

DNA methylation in the pathophysiology of hyperphenylalaninemia in the PAH(enu2) mouse model of phenylketonuria.
Dobrowolski SF, Lyons-Weiler J, Spridik K, Vockley J, Skvorak K, Biery A
Molecular genetics and metabolism119(1-2)1-7(2016 09)

Adaptor protein LRAP25 mediates myotonic dystrophy kinase-related Cdc42-binding kinase (MRCK) regulation of LIMK1 protein in lamellipodial F-actin dynamics.
Lee IC, Leung T, Tan I
The Journal of biological chemistry289(39)26989-7003(2014 Sep)

Insm1 promotes endocrine cell differentiation by modulating the expression of a network of genes that includes Neurog3 and Ripply3.
Osipovich AB, Long Q, Manduchi E, Gangula R, Hipkens SB, Schneider J, Okubo T, Stoeckert CJ Jr, Takada S, Magnuson MA
Development (Cambridge, England)141(15)2939-49(2014 Aug)

Nuclear movement regulated by Cdc42, MRCK, myosin, and actin flow establishes MTOC polarization in migrating cells.
Gomes ER, Jani S, Gundersen GG
Cell121(3)451-63(2005 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cdc42bpa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cdc42bpa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu77094	NM_001359543.1 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu77096	NM_001359544.1 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu77097	NM_001359546.1 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu77098	NM_001359547.1 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu77099	NM_001359548.1 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu77101	NM_001359549.1 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu28721	XM_017320119.2 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu28722	XM_006496802.4 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu28723	XM_006496803.4 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu28724	XM_006496804.4 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu28726	XM_006496806.4 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu28727	XM_006496807.4 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu28729	XM_006496809.4 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu73308	XM_017320130.2 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu73308	XM_011238803.3 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu73309	XM_011238804.3 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu77094	XM_017320127.1 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu77095	XM_017320129.2 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu77096	XM_017320131.1 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu77097	XM_017320132.1 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu77098	XM_017320134.1 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu77099	XM_017320142.1 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu77100	XM_017320145.1 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu77101	XM_017320146.1 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu77102	XM_017320148.1 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu85130	XM_030253509.1 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu85131	XM_030253510.1 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu12565	NM_001033285.1 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu28725	NM_001359541.1 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu28728	NM_001359542.1 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu28730	NM_001346804.1 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu28732	NM_001359545.1 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu28733	NM_001346805.1 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu28721	XM_006496801.3 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu28725	XM_006496805.3 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu28728	XM_006496808.3 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu28732	XM_006496812.3 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu73307	XM_011238802.2 Latest version!	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OMu77095	XM_017320129.1	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu73308	XM_011238803.2	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu28722	XM_006496802.3	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu28724	XM_006496804.3	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu28723	XM_006496803.3	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu73308	XM_017320130.1	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu73309	XM_011238804.2	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu28729	XM_006496809.3	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu28721	XM_017320119.1	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu28726	XM_006496806.3	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu28727	XM_006496807.3	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cdc42bpa ( NM_001359543.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346472.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OMu77094
Clone ID Related Accession (Same CDS sequence)	XM_017320127.1 , NM_001359543.1
Accession Version	NM_001359543.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform 6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC125380.4 and AC132436.3. On Jan 13, 2018 this sequence version replaced XM_017320127.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.239198.1, SRR1660821.46787.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAAGATTCC CTTTCTTCTT CCTCATCCTC ACTGATTGAT 
TTTTTGGATG ACATTGCAGA CTGTGCTCCA CTTCCAGCTC ACACACCAAC CTTAAGGAAA 
AAGGGATGCC CGGCTTCAAC TGGCTTTCCA CCTAAGCGCA AGACTCATCA ATTTTTCGTG 
AAGTCTTTCA CTGCCCCTAC CAAGTGTCAC CAGTGCACCT CCTTGATGGT CGGTTTGATA 
AGACAGGGCT GTTCCTGTGA AGTCTGTGGG TTCTCATGTC ATATAACTTG TGTGAACAAA 
GCCCCGACGG TTTGCCCAGT TCCTCCTGAG CAAACTAAAG GTCCACTGGG CATAGACCCC 
CAGAAGGGAG TTGGGACGGC GTATGAGGGT CATGTCAGGA TCCCTAAGCC GGCGGGAGTG 
AAGAAAGGGT GGCAGAGAGC ACTGGCTGTG GTCTGCGACT TCAAACTGTT TCTTTATGAT 
ATCGCAGAGG GAAAAGCATC GCAGCCCACC TCCGTCATTA GTCAAGTGAT TGACATGAGA 
GATGAAGAAT TTTCTGTGAG TTCAGTCTTA GCTTCTGATG TTATTCACGC AAGTCGAAAA 
GATATCCCCT GTATATTCAG GGTCACAGCT TCCCAGCTCT CAGCACCTAG TAACAAATGT 
TCCATCCTGA TGCTAGCAGA CAGCGAGAAT GAGAGGAGTA AGTGGGTGGG AGTGCTGAGT 
GAACTCCACA AGATTCTGAA GAAAAACAAG TTCAGAGACC GCTCAGTGTA TGTCCCCAAG 
GAGGCGTACG ACAGCACGCT ACCCCTCATT AAGACAACGC AGGCCGCTGC AATCATAGAT 
CATGAACGGA TAGCTTTGGG AAATGAAGAA GGATTATTTG TTGTGCATGT CACCAAAGAT 
GAGATTGTTA GAGTTGGTGA TAATAAGAAA ATCCATCAGA TCGAACTCAT TCCAAGTGAC 
CAGCTTGTCG CTGTGATCTC AGGCCGAAAT CGCCACGTCC GACTTTTTCC TATGTCAGCC 
TTGGATGGGC GAGAGACGGA TTTTTATAAA CTGGCAGAGA CTAAAGGGTG TCAGACCATA 
GCTGCTGGGA AAGTGCGCCA CGGAGCTCTG TCCTGCCTGT GTGTGGCCAT GAAGAGACAG 
GTCCTCTGCT ATGAACTGTT CCAAAGCAAG ACCAGGCACA GGAAGTTTAA GGAGATTCAA 
GTCCCATGTA ATGTCCAGTG GATGGCCATC TTCAGTGAGC ATCTCTGTGT GGGATTCCAG 
TCAGGATTTC TCAGATACCC CCTGAATGGA GAAGGAGGTC CATGCAATAT GCTCCATTCA 
AATGACCATA CACTGTCATT CATTTCACAC CAACCAATGG ACGCTCTCTG TGCAGTTGAG 
ATCTCCAACA AAGAGTACTT GCTGTGTTTT AACAGCATTG GGATATACAC AGACTGCCAG 
GGCCGAAGAT CCAGACAGCA GGAACTGATG TGGCCAGCAA ATCCTTCCTC CTGTTGTTAC 
AATGCTCCAT ATCTCTCCGT GTATAGTGAA AACGCAGTTG ATATCTTTGA TGTAAACTCC 
ATGGAATGGA TACAGACTCT TCCTCTCAAA AAGGTCCGAC CTTTAAACAC TGAAGGATCA 
TTAAATCTCT TAGGATTGGA GACCATTAGA TTAATATATT TCAAAAATAA GATGGCAGAA 
GGGGATGAAC TTGTGGTTCC TGAAACATCA GATAATAGTC GGAAACAAAT GGTTAGAAAT 
ATCAACAACA AACGGCGTTA CTCCTTCCGA GTCCCTGAAG AAGAGAGGAT GCAACAGAGG 
AGGGAGATGC TACGAGATCC AGAAATGAGA AATAAATTAA TTTCTAACCC AACTAATTTT 
AACCACATAG CACACATGGG TCCTGGAGAT GGAATACAGA TCCTAAAAGA CCTGCCCATG 
AACCCACGGC CTCAGGAAAG CCGGACAGTA TTCAGTGGCT CCGTCAGTAT TCCCTCCATC 
ACCAAGTCCC GCCCTGAGCC AGGCCGCTCC ATGAGTGCCA GCAGCGGCCT GTCTGCACGG 
TCGTCAGCCC AGAATGGCAG TGCACTAAAG AGGGAATTCT CTGGAGGAAG CTATAACACA 
AAGCGACAGC CCATGCCCTC TCCCTCGGAG GGCTCTTTGT CATCAGGAGG CATGGACCAA 
GGAAGTGACG CCCCTGCGAG GGACTATGAT GGAGAGGACT CTGATTCTCC AAGGCATTCC 
ACAGCTTCCA ACAGCTCTAA CCTGAGCAGC CCCCCAAGTC CCATTTCACC CCAAAAGACC 
AAGAGCCTCT CCCTGGAGAG CACAGACCGC GGGAGCTGGG ATCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346472.1
CDS	351..5558
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSLSSSSSSLIDFLDDIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	NM_001359543.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359543.1

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAAGATTCC CTTTCTTCTT CCTCATCCTC ACTGATTGAT 
TTTTTGGATG ACATTGCAGA CTGTGCTCCA CTTCCAGCTC ACACACCAAC CTTAAGGAAA 
AAGGGATGCC CGGCTTCAAC TGGCTTTCCA CCTAAGCGCA AGACTCATCA ATTTTTCGTG 
AAGTCTTTCA CTGCCCCTAC CAAGTGTCAC CAGTGCACCT CCTTGATGGT CGGTTTGATA 
AGACAGGGCT GTTCCTGTGA AGTCTGTGGG TTCTCATGTC ATATAACTTG TGTGAACAAA 
GCCCCGACGG TTTGCCCAGT TCCTCCTGAG CAAACTAAAG GTCCACTGGG CATAGACCCC 
CAGAAGGGAG TTGGGACGGC GTATGAGGGT CATGTCAGGA TCCCTAAGCC GGCGGGAGTG 
AAGAAAGGGT GGCAGAGAGC ACTGGCTGTG GTCTGCGACT TCAAACTGTT TCTTTATGAT 
ATCGCAGAGG GAAAAGCATC GCAGCCCACC TCCGTCATTA GTCAAGTGAT TGACATGAGA 
GATGAAGAAT TTTCTGTGAG TTCAGTCTTA GCTTCTGATG TTATTCACGC AAGTCGAAAA 
GATATCCCCT GTATATTCAG GGTCACAGCT TCCCAGCTCT CAGCACCTAG TAACAAATGT 
TCCATCCTGA TGCTAGCAGA CAGCGAGAAT GAGAGGAGTA AGTGGGTGGG AGTGCTGAGT 
GAACTCCACA AGATTCTGAA GAAAAACAAG TTCAGAGACC GCTCAGTGTA TGTCCCCAAG 
GAGGCGTACG ACAGCACGCT ACCCCTCATT AAGACAACGC AGGCCGCTGC AATCATAGAT 
CATGAACGGA TAGCTTTGGG AAATGAAGAA GGATTATTTG TTGTGCATGT CACCAAAGAT 
GAGATTGTTA GAGTTGGTGA TAATAAGAAA ATCCATCAGA TCGAACTCAT TCCAAGTGAC 
CAGCTTGTCG CTGTGATCTC AGGCCGAAAT CGCCACGTCC GACTTTTTCC TATGTCAGCC 
TTGGATGGGC GAGAGACGGA TTTTTATAAA CTGGCAGAGA CTAAAGGGTG TCAGACCATA 
GCTGCTGGGA AAGTGCGCCA CGGAGCTCTG TCCTGCCTGT GTGTGGCCAT GAAGAGACAG 
GTCCTCTGCT ATGAACTGTT CCAAAGCAAG ACCAGGCACA GGAAGTTTAA GGAGATTCAA 
GTCCCATGTA ATGTCCAGTG GATGGCCATC TTCAGTGAGC ATCTCTGTGT GGGATTCCAG 
TCAGGATTTC TCAGATACCC CCTGAATGGA GAAGGAGGTC CATGCAATAT GCTCCATTCA 
AATGACCATA CACTGTCATT CATTTCACAC CAACCAATGG ACGCTCTCTG TGCAGTTGAG 
ATCTCCAACA AAGAGTACTT GCTGTGTTTT AACAGCATTG GGATATACAC AGACTGCCAG 
GGCCGAAGAT CCAGACAGCA GGAACTGATG TGGCCAGCAA ATCCTTCCTC CTGTTGTTAC 
AATGCTCCAT ATCTCTCCGT GTATAGTGAA AACGCAGTTG ATATCTTTGA TGTAAACTCC 
ATGGAATGGA TACAGACTCT TCCTCTCAAA AAGGTCCGAC CTTTAAACAC TGAAGGATCA 
TTAAATCTCT TAGGATTGGA GACCATTAGA TTAATATATT TCAAAAATAA GATGGCAGAA 
GGGGATGAAC TTGTGGTTCC TGAAACATCA GATAATAGTC GGAAACAAAT GGTTAGAAAT 
ATCAACAACA AACGGCGTTA CTCCTTCCGA GTCCCTGAAG AAGAGAGGAT GCAACAGAGG 
AGGGAGATGC TACGAGATCC AGAAATGAGA AATAAATTAA TTTCTAACCC AACTAATTTT 
AACCACATAG CACACATGGG TCCTGGAGAT GGAATACAGA TCCTAAAAGA CCTGCCCATG 
AACCCACGGC CTCAGGAAAG CCGGACAGTA TTCAGTGGCT CCGTCAGTAT TCCCTCCATC 
ACCAAGTCCC GCCCTGAGCC AGGCCGCTCC ATGAGTGCCA GCAGCGGCCT GTCTGCACGG 
TCGTCAGCCC AGAATGGCAG TGCACTAAAG AGGGAATTCT CTGGAGGAAG CTATAACACA 
AAGCGACAGC CCATGCCCTC TCCCTCGGAG GGCTCTTTGT CATCAGGAGG CATGGACCAA 
GGAAGTGACG CCCCTGCGAG GGACTATGAT GGAGAGGACT CTGATTCTCC AAGGCATTCC 
ACAGCTTCCA ACAGCTCTAA CCTGAGCAGC CCCCCAAGTC CCATTTCACC CCAAAAGACC 
AAGAGCCTCT CCCTGGAGAG CACAGACCGC GGGAGCTGGG ATCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( NM_001359544.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346473.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 7, mRNA.

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CloneID	OMu77096
Clone ID Related Accession (Same CDS sequence)	XM_017320131.1 , NM_001359544.1
Accession Version	NM_001359544.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform 7
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC125380.4 and AC132436.3. On Jan 13, 2018 this sequence version replaced XM_017320131.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.105431.1, SRR1660817.23075.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGCTG GAAGTTCACA CCGAAGCTCT AATTGCTGAA 
GCATCAAAGG ACAAGAAGCT CCGAGAGCAG AGCGAACATT ATTCTAAGCA ACTGGAAAAC 
GAGTTGGAGG GCCTGAAGCA AAAACAAATT AGTTACTCAC CAGGAATATG CAGCATAGAA 
CATCAGCAAG AGATAACTAA ACTGAAAACT GACTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAAATTT CTAAAAGAGA AGGAATTCAC GCAAGTGAGA TTAAAAACCT GAAGAAGGAG 
CTGCATGACT CAGAAGGCCA GCAGCTTGCT CTCAACAAAG AGATTCTGGT TTTAAAAGAC 
AAATTGGAAA AAACCAGGAG AGAAAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAACAACAAT ATGAACGGGA AAAAGTATTA TTAACTGAAG AGAATAAAAA GTTAACGAGT 
GAACTCGATA AGCTGACTTC ACTGTATGAG AGCTTAAGTT TGCGCAACCA GCACTTAGAA 
GAAGAAGTCA AAGATCTAGC AGATAAGAAG GAATCAGTTG CCCACTGGGA AGCCCAAATC 
ACAGAGATAA TTCAGTGGGT GAGCGATGAG AAGGATGCAC GAGGCTATCT TCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA GTTGGAAGCA TTAAGAAATT CCAGCTTGGG GACACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCAAAACTGG ACATGTCGGC TAGACTAGAG 
TTGCAGTCTG CTCTGGATGC AGAAATAAGA GCTAAGCAGG CCATCCAGGA AGAGCTGAAT 
AAAGTCAAAG CATCTAACAT CTTAACAGAA TGTAAACTGA AAGATTCAGA GAAGAAGAAC 
TTGGAACTGT TGTCCGAAAT CGAGCAGCTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ACCAATTTGA AATTGCAGAC TGTGCTCCAC TTCCAGCTCA CACACCAACC 
TTAAGGAAAA AGGGATGCCC GGCTTCAACT GGCTTTCCAC CTAAGCGCAA GACTCATCAA 
TTTTTCGTGA AGTCTTTCAC TGCCCCTACC AAGTGTCACC AGTGCACCTC CTTGATGGTC 
GGTTTGATAA GACAGGGCTG TTCCTGTGAA GTCTGTGGGT TCTCATGTCA TATAACTTGT 
GTGAACAAAG CCCCGACGGT TTGCCCAGTT CCTCCTGAGC AAACTAAAGG TCCACTGGGC 
ATAGACCCCC AGAAGGGAGT TGGGACGGCG TATGAGGGTC ATGTCAGGAT CCCTAAGCCG 
GCGGGAGTGA AGAAAGGGTG GCAGAGAGCA CTGGCTGTGG TCTGCGACTT CAAACTGTTT 
CTTTATGATA TCGCAGAGGG AAAAGCATCG CAGCCCACCT CCGTCATTAG TCAAGTGATT 
GACATGAGAG ATGAAGAATT TTCTGTGAGT TCAGTCTTAG CTTCTGATGT TATTCACGCA 
AGTCGAAAAG ATATCCCCTG TATATTCAGG GTCACAGCTT CCCAGCTCTC AGCACCTAGT 
AACAAATGTT CCATCCTGAT GCTAGCAGAC AGCGAGAATG AGAGGAGTAA GTGGGTGGGA 
GTGCTGAGTG AACTCCACAA GATTCTGAAG AAAAACAAGT TCAGAGACCG CTCAGTGTAT 
GTCCCCAAGG AGGCGTACGA CAGCACGCTA CCCCTCATTA AGACAACGCA GGCCGCTGCA 
ATCATAGATC ATGAACGGAT AGCTTTGGGA AATGAAGAAG GATTATTTGT TGTGCATGTC 
ACCAAAGATG AGATTGTTAG AGTTGGTGAT AATAAGAAAA TCCATCAGAT CGAACTCATT 
CCAAGTGACC AGCTTGTCGC TGTGATCTCA GGCCGAAATC GCCACGTCCG ACTTTTTCCT 
ATGTCAGCCT TGGATGGGCG AGAGACGGAT TTTTATAAAC TGGCAGAGAC TAAAGGGTGT 
CAGACCATAG CTGCTGGGAA AGTGCGCCAC GGAGCTCTGT CCTGCCTGTG TGTGGCCATG 
AAGAGACAGG TCCTCTGCTA TGAACTGTTC CAAAGCAAGA CCAGGCACAG GAAGTTTAAG 
GAGATTCAAG TCCCATGTAA TGTCCAGTGG ATGGCCATCT TCAGTGAGCA TCTCTGTGTG 
GGATTCCAGT CAGGATTTCT CAGATACCCC CTGAATGGAG AAGGAGGTCC ATGCAATATG 
CTCCATTCAA ATGACCATAC ACTGTCATTC ATTTCACACC AACCAATGGA CGCTCTCTGT 
GCAGTTGAGA TCTCCAACAA AGAGTACTTG CTGTGTTTTA ACAGCATTGG GATATACACA 
GACTGCCAGG GCCGAAGATC CAGACAGCAG GAACTGATGT GGCCAGCAAA TCCTTCCTCC 
TGTTGTTACA ATGCTCCATA TCTCTCCGTG TATAGTGAAA ACGCAGTTGA TATCTTTGAT 
GTAAACTCCA TGGAATGGAT ACAGACTCTT CCTCTCAAAA AGGTCCGACC TTTAAACACT 
GAAGGATCAT TAAATCTCTT AGGATTGGAG ACCATTAGAT TAATATATTT CAAAAATAAG 
ATGGCAGAAG GGGATGAACT TGTGGTTCCT GAAACATCAG ATAATAGTCG GAAACAAATG 
GTTAGAAATA TCAACAACAA ACGGCGTTAC TCCTTCCGAG TCCCTGAAGA AGAGAGGATG 
CAACAGAGGA GGGAGATGCT ACGAGATCCA GAAATGAGAA ATAAATTAAT TTCTAACCCA 
ACTAATTTTA ACCACATAGC ACACATGGGT CCTGGAGATG GAATACAGAT CCTAAAAGAC 
CTGCCCATGC CAGGTTTCCC TTATTCACCC CCTCATCACC ACTCCGGCCT GATCTCCTCT 
CCGAGCAACT TTGAGCACAT CTATCACATG ACTGTCACTT CTGCTGAACA GTTTCTCTCT 
CCTGAGTCCA TAAGCCCTGG GTCCCCCCCG TCCCTGCGCT CTGCCCCTGG CACACCACAC 
TCTGTGACTC TGAGGAACCC ACGGCCTCAG GAAAGCCGGA CAGTATTCAG TGGCTCCGTC 
AGTATTCCCT CCATCACCAA GTCCCGCCCT GAGCCAGGCC GCTCCATGAG TGCCAGCAGC 
GGCCTGTCTG CACGGTCGTC AGCCCAGAAT GGCAGTGCAC TAAAGAGGGA ATTCTCTGGA 
GGAAGCTATA ACACAAAGCG ACAGCCCATG CCCTCTCCCT CGGAGGGCTC TTTGTCATCA 
GGAGGCATGG ACCAAGGAAG TGACGCCCCT GCGAGGGACT ATGATGGAGA GGACTCTGAT 
TCTCCAAGGC ATTCCACAGC TTCCAACAGC TCTAACCTGA GCAGCCCCCC AAGTCCCATT 
TCACCCCAAA AGACCAAGAG CCTCTCCCTG GAGAGCACAG ACCGCGGGAG CTGGGATCCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346473.1
CDS	351..5453
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKLEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	NM_001359544.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359544.1

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGCTG GAAGTTCACA CCGAAGCTCT AATTGCTGAA 
GCATCAAAGG ACAAGAAGCT CCGAGAGCAG AGCGAACATT ATTCTAAGCA ACTGGAAAAC 
GAGTTGGAGG GCCTGAAGCA AAAACAAATT AGTTACTCAC CAGGAATATG CAGCATAGAA 
CATCAGCAAG AGATAACTAA ACTGAAAACT GACTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAAATTT CTAAAAGAGA AGGAATTCAC GCAAGTGAGA TTAAAAACCT GAAGAAGGAG 
CTGCATGACT CAGAAGGCCA GCAGCTTGCT CTCAACAAAG AGATTCTGGT TTTAAAAGAC 
AAATTGGAAA AAACCAGGAG AGAAAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAACAACAAT ATGAACGGGA AAAAGTATTA TTAACTGAAG AGAATAAAAA GTTAACGAGT 
GAACTCGATA AGCTGACTTC ACTGTATGAG AGCTTAAGTT TGCGCAACCA GCACTTAGAA 
GAAGAAGTCA AAGATCTAGC AGATAAGAAG GAATCAGTTG CCCACTGGGA AGCCCAAATC 
ACAGAGATAA TTCAGTGGGT GAGCGATGAG AAGGATGCAC GAGGCTATCT TCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA GTTGGAAGCA TTAAGAAATT CCAGCTTGGG GACACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCAAAACTGG ACATGTCGGC TAGACTAGAG 
TTGCAGTCTG CTCTGGATGC AGAAATAAGA GCTAAGCAGG CCATCCAGGA AGAGCTGAAT 
AAAGTCAAAG CATCTAACAT CTTAACAGAA TGTAAACTGA AAGATTCAGA GAAGAAGAAC 
TTGGAACTGT TGTCCGAAAT CGAGCAGCTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ACCAATTTGA AATTGCAGAC TGTGCTCCAC TTCCAGCTCA CACACCAACC 
TTAAGGAAAA AGGGATGCCC GGCTTCAACT GGCTTTCCAC CTAAGCGCAA GACTCATCAA 
TTTTTCGTGA AGTCTTTCAC TGCCCCTACC AAGTGTCACC AGTGCACCTC CTTGATGGTC 
GGTTTGATAA GACAGGGCTG TTCCTGTGAA GTCTGTGGGT TCTCATGTCA TATAACTTGT 
GTGAACAAAG CCCCGACGGT TTGCCCAGTT CCTCCTGAGC AAACTAAAGG TCCACTGGGC 
ATAGACCCCC AGAAGGGAGT TGGGACGGCG TATGAGGGTC ATGTCAGGAT CCCTAAGCCG 
GCGGGAGTGA AGAAAGGGTG GCAGAGAGCA CTGGCTGTGG TCTGCGACTT CAAACTGTTT 
CTTTATGATA TCGCAGAGGG AAAAGCATCG CAGCCCACCT CCGTCATTAG TCAAGTGATT 
GACATGAGAG ATGAAGAATT TTCTGTGAGT TCAGTCTTAG CTTCTGATGT TATTCACGCA 
AGTCGAAAAG ATATCCCCTG TATATTCAGG GTCACAGCTT CCCAGCTCTC AGCACCTAGT 
AACAAATGTT CCATCCTGAT GCTAGCAGAC AGCGAGAATG AGAGGAGTAA GTGGGTGGGA 
GTGCTGAGTG AACTCCACAA GATTCTGAAG AAAAACAAGT TCAGAGACCG CTCAGTGTAT 
GTCCCCAAGG AGGCGTACGA CAGCACGCTA CCCCTCATTA AGACAACGCA GGCCGCTGCA 
ATCATAGATC ATGAACGGAT AGCTTTGGGA AATGAAGAAG GATTATTTGT TGTGCATGTC 
ACCAAAGATG AGATTGTTAG AGTTGGTGAT AATAAGAAAA TCCATCAGAT CGAACTCATT 
CCAAGTGACC AGCTTGTCGC TGTGATCTCA GGCCGAAATC GCCACGTCCG ACTTTTTCCT 
ATGTCAGCCT TGGATGGGCG AGAGACGGAT TTTTATAAAC TGGCAGAGAC TAAAGGGTGT 
CAGACCATAG CTGCTGGGAA AGTGCGCCAC GGAGCTCTGT CCTGCCTGTG TGTGGCCATG 
AAGAGACAGG TCCTCTGCTA TGAACTGTTC CAAAGCAAGA CCAGGCACAG GAAGTTTAAG 
GAGATTCAAG TCCCATGTAA TGTCCAGTGG ATGGCCATCT TCAGTGAGCA TCTCTGTGTG 
GGATTCCAGT CAGGATTTCT CAGATACCCC CTGAATGGAG AAGGAGGTCC ATGCAATATG 
CTCCATTCAA ATGACCATAC ACTGTCATTC ATTTCACACC AACCAATGGA CGCTCTCTGT 
GCAGTTGAGA TCTCCAACAA AGAGTACTTG CTGTGTTTTA ACAGCATTGG GATATACACA 
GACTGCCAGG GCCGAAGATC CAGACAGCAG GAACTGATGT GGCCAGCAAA TCCTTCCTCC 
TGTTGTTACA ATGCTCCATA TCTCTCCGTG TATAGTGAAA ACGCAGTTGA TATCTTTGAT 
GTAAACTCCA TGGAATGGAT ACAGACTCTT CCTCTCAAAA AGGTCCGACC TTTAAACACT 
GAAGGATCAT TAAATCTCTT AGGATTGGAG ACCATTAGAT TAATATATTT CAAAAATAAG 
ATGGCAGAAG GGGATGAACT TGTGGTTCCT GAAACATCAG ATAATAGTCG GAAACAAATG 
GTTAGAAATA TCAACAACAA ACGGCGTTAC TCCTTCCGAG TCCCTGAAGA AGAGAGGATG 
CAACAGAGGA GGGAGATGCT ACGAGATCCA GAAATGAGAA ATAAATTAAT TTCTAACCCA 
ACTAATTTTA ACCACATAGC ACACATGGGT CCTGGAGATG GAATACAGAT CCTAAAAGAC 
CTGCCCATGC CAGGTTTCCC TTATTCACCC CCTCATCACC ACTCCGGCCT GATCTCCTCT 
CCGAGCAACT TTGAGCACAT CTATCACATG ACTGTCACTT CTGCTGAACA GTTTCTCTCT 
CCTGAGTCCA TAAGCCCTGG GTCCCCCCCG TCCCTGCGCT CTGCCCCTGG CACACCACAC 
TCTGTGACTC TGAGGAACCC ACGGCCTCAG GAAAGCCGGA CAGTATTCAG TGGCTCCGTC 
AGTATTCCCT CCATCACCAA GTCCCGCCCT GAGCCAGGCC GCTCCATGAG TGCCAGCAGC 
GGCCTGTCTG CACGGTCGTC AGCCCAGAAT GGCAGTGCAC TAAAGAGGGA ATTCTCTGGA 
GGAAGCTATA ACACAAAGCG ACAGCCCATG CCCTCTCCCT CGGAGGGCTC TTTGTCATCA 
GGAGGCATGG ACCAAGGAAG TGACGCCCCT GCGAGGGACT ATGATGGAGA GGACTCTGAT 
TCTCCAAGGC ATTCCACAGC TTCCAACAGC TCTAACCTGA GCAGCCCCCC AAGTCCCATT 
TCACCCCAAA AGACCAAGAG CCTCTCCCTG GAGAGCACAG ACCGCGGGAG CTGGGATCCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( NM_001359546.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346475.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 9, mRNA.

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CloneID	OMu77097
Clone ID Related Accession (Same CDS sequence)	XM_017320132.1 , NM_001359546.1
Accession Version	NM_001359546.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform 9
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC125380.4 and AC132436.3. On Jan 13, 2018 this sequence version replaced XM_017320132.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.141634.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGATTAC ATAGAGAAGA CTTTGAAATA TTAAAGGTGA TTGGTCGAGG AGCTTTTGGG 
GAGGTTGCTG TAGTTAAACT AAAAAATGCA GATAAAGTAT TCGCCATGAA AATTCTGAAC 
AAGTGGGAAA TGCTGAAAAG AGCTGAGACA GCGTGCTTTC GTGAAGAGAG GGATGTGTTG 
GTAAATGGAG ACAGTAAGTG GATAACCACT CTGCACTATG CTTTCCAGGA TGACAATAAC 
TTATACCTGG TTATGGATTA TTATGTTGGT GGGGATTTAC TTACTCTGCT CAGCAAATTT 
GAAGATAGAT TGCCTGAAGA GATGGCTCGG TTTTACTTGG CTGAGATGGT GATAGCCATT 
GACTCAGTTC ACCAGCTCCA CTATGTTCAC AGAGACATCA AACCTGACAA TATATTGATG 
GATATGAATG GACATATTCG TTTAGCAGAT TTTGGTTCTT GTCTGAAGCT GATGGAAGAT 
GGAACGGTCC AGTCCTCGGT GGCAGTTGGA ACTCCAGATT ATATTTCTCC GGAAATCCTT 
CAGGCTATGG AAGACGGAAA AGGCAGATAT GGACCAGAGT GTGACTGGTG GTCCTTGGGG 
GTCTGTATGT ATGAGATGCT TTACGGAGAA ACACCATTCT ATGCAGAGTC TCTGGTGGAG 
ACATATGGAA AAATTATGAA TCACAAAGAG CGGTTTCAGT TTCCAGCCCA AGTGACAGAC 
GTGTCCGAAA ATGCTAAAGA TCTTATTCGA AGACTCATTT GTAGTAGAGA ACATCGACTT 
GGCCAAAATG GAATAGAAGA CTTTAAGAAG CATCCATTCT TCAGTGGAAT TGATTGGGAT 
AACATTCGGA ATTGTGAAGC ACCTTACATC CCAGAAGTTA GTAGCCCGAC AGATACGTCA 
AATTTTGATG TGGATGACGA CTGTTTAAAA AATTCTGAAA CAATGCCCCC ACCAACACAT 
ACTGCATTTT CTGGCCATCA TCTACCATTT GTTGGTTTTA CATATACTAG TAGCTGTGTT 
CTTTCTGATC GGAGCTGTTT AAGAGTCACA GCAGGCCCCA CCTCATTGGA CCTTGATGTC 
AGTGTCCAGA GGACTCTAGA CAATAACTTA GCAACTGAGG CTTATGAAAG GAGAATTAAG 
CGGCTTGAGC AGGAAAAGCT TGAACTGACT AGAAAACTTC AAGAGTCAAC ACAGACTGTT 
CAAGCTCTGC AATATTCAAC TGTTGATGGT CCACTAACGG CAAGCAAAGA TTTAGAAATT 
AAAAGTCTGA AAGAAGAGAT TGAAAAACTA AGGAAACAAG TAGCAGAAGT GAATCATTTG 
GAACAACAAC TTGAAGAAGC TAATTCTGTG AGACGAGAAC TAGATGATGC TTTCAGGCAA 
ATCAAAGCTT CTGAAAAACA AATCAAAACT TTGCAGCAGG AAAGAGAAGA ACTAAATAAG 
GAACTAGTCC AGGCTAGTGA GCGATTAAAA AACCAATCCA AAGAGCTGAA AGATGCACAC 
TGTCAGAGGA AACTGGCCAT GCAGGAATTC ATGGAGATCA ATGAGCGACT AACAGAATTG 
CACACCCAAA AGCAGAAACT TGCTCGCCAT GTCCGAGATA AGGAAGAAGA GGTGGACCTG 
GTGATGCAAA AAGCTGAGAG CTTAAGGCAG GAGCTGCGCA GAGCAGAAAG AGCCAAGAAA 
GAGCTGGAAG TTCACACCGA AGCTCTAATT GCTGAAGCAT CAAAGGACAA GAAGCTCCGA 
GAGCAGAGCG AACATTATTC TAAGCAACTG GAAAACGAGT TGGAGGGCCT GAAGCAAAAA 
CAAATTAGTT ACTCACCAGG AATATGCAGC ATAGAACATC AGCAAGAGAT AACTAAACTG 
AAAACTGACT TGGAAAAGAA AAGTATCTTT TATGAAGAAG AAATTTCTAA AAGAGAAGGA 
ATTCACGCAA GTGAGATTAA AAACCTGAAG AAGGAGCTGC ATGACTCAGA AGGCCAGCAG 
CTTGCTCTCA ACAAAGAGAT TCTGGTTTTA AAAGACAAAT TGGAAAAAAC CAGGAGAGAA 
AGTCAAAGTG AAAGGGAAGA ATTTGAAAAT GAGTTCAAAC AACAATATGA ACGGGAAAAA 
GTATTATTAA CTGAAGAGAA TAAAAAGTTA ACGAGTGAAC TCGATAAGCT GACTTCACTG 
TATGAGAGCT TAAGTTTGCG CAACCAGCAC TTAGAAGAAG AAGTCAAAGA TCTAGCAGAT 
AAGAAGGAAT CAGTTGCCCA CTGGGAAGCC CAAATCACAG AGATAATTCA GTGGGTGAGC 
GATGAGAAGG ATGCACGAGG CTATCTTCAG GCCTTAGCTT CTAAAATGAC TGAAGAGTTG 
GAAGCATTAA GAAATTCCAG CTTGGGGACA CGAGCAACAG ATATGCCCTG GAAAATGCGT 
CGTTTTGCAA AACTGGACAT GTCGGCTAGA CTAGAGTTGC AGTCTGCTCT GGATGCAGAA 
ATAAGAGCTA AGCAGGCCAT CCAGGAAGAG CTGAATAAAG TCAAAGCATC TAACATCTTA 
ACAGAATGTA AACTGAAAGA TTCAGAGAAG AAGAACTTGG AACTGTTGTC CGAAATCGAG 
CAGCTGATAA AGGACACTGA AGAGCTTAGA TCTGAAAAGG GTATAGAGCA CCAAGACTCA 
CAGCATTCTT TCTTGGCATT TTTGAATACG CCTACCGATG CTCTGGACCA ATTTGAACGC 
TCTCCGTCCT GTACTCCAGC TAGCAAAGGC AGACGTATTG CAGACTGTGC TCCACTTCCA 
GCTCACACAC CAACCTTAAG GAAAAAGGGA TGCCCGGCTT CAACTGGCTT TCCACCTAAG 
CGCAAGACTC ATCAATTTTT CGTGAAGTCT TTCACTGCCC CTACCAAGTG TCACCAGTGC 
ACCTCCTTGA TGGTCGGTTT GATAAGACAG GGCTGTTCCT GTGAAGTCTG TGGGTTCTCA 
TGTCATATAA CTTGTGTGAA CAAAGCCCCG ACGGTTTGCC CAGTTCCTCC TGAGCAAACT 
AAAGGTCCAC TGGGCATAGA CCCCCAGAAG GGAGTTGGGA CGGCGTATGA GGGTCATGTC 
AGGATCCCTA AGCCGGCGGG AGTGAAGAAA GGGTGGCAGA GAGCACTGGC TGTGGTCTGC 
GACTTCAAAC TGTTTCTTTA TGATATCGCA GAGGGAAAAG CATCGCAGCC CACCTCCGTC 
ATTAGTCAAG TGATTGACAT GAGAGATGAA GAATTTTCTG TGAGTTCAGT CTTAGCTTCT 
GATGTTATTC ACGCAAGTCG AAAAGATATC CCCTGTATAT TCAGGGTCAC AGCTTCCCAG 
CTCTCAGCAC CTAGTAACAA ATGTTCCATC CTGATGCTAG CAGACAGCGA GAATGAGAGG 
AGTAAGTGGG TGGGAGTGCT GAGTGAACTC CACAAGATTC TGAAGAAAAA CAAGTTCAGA 
GACCGCTCAG TGTATGTCCC CAAGGAGGCG TACGACAGCA CGCTACCCCT CATTAAGACA 
ACGCAGGCCG CTGCAATCAT AGATCATGAA CGGATAGCTT TGGGAAATGA AGAAGGATTA 
TTTGTTGTGC ATGTCACCAA AGATGAGATT GTTAGAGTTG GTGATAATAA GAAAATCCAT 
CAGATCGAAC TCATTCCAAG TGACCAGCTT GTCGCTGTGA TCTCAGGCCG AAATCGCCAC 
GTCCGACTTT TTCCTATGTC AGCCTTGGAT GGGCGAGAGA CGGATTTTTA TAAACTGGCA 
GAGACTAAAG GGTGTCAGAC CATAGCTGCT GGGAAAGTGC GCCACGGAGC TCTGTCCTGC 
CTGTGTGTGG CCATGAAGAG ACAGGTCCTC TGCTATGAAC TGTTCCAAAG CAAGACCAGG 
CACAGGAAGT TTAAGGAGAT TCAAGTCCCA TGTAATGTCC AGTGGATGGC CATCTTCAGT 
GAGCATCTCT GTGTGGGATT CCAGTCAGGA TTTCTCAGAT ACCCCCTGAA TGGAGAAGGA 
GGTCCATGCA ATATGCTCCA TTCAAATGAC CATACACTGT CATTCATTTC ACACCAACCA 
ATGGACGCTC TCTGTGCAGT TGAGATCTCC AACAAAGAGT ACTTGCTGTG TTTTAACAGC 
ATTGGGATAT ACACAGACTG CCAGGGCCGA AGATCCAGAC AGCAGGAACT GATGTGGCCA 
GCAAATCCTT CCTCCTGTTG TTACAATGCT CCATATCTCT CCGTGTATAG TGAAAACGCA 
GTTGATATCT TTGATGTAAA CTCCATGGAA TGGATACAGA CTCTTCCTCT CAAAAAGGTC 
CGACCTTTAA ACACTGAAGG ATCATTAAAT CTCTTAGGAT TGGAGACCAT TAGATTAATA 
TATTTCAAAA ATAAGATGGC AGAAGGGGAT GAACTTGTGG TTCCTGAAAC ATCAGATAAT 
AGTCGGAAAC AAATGGTTAG AAATATCAAC AACAAACGGC GTTACTCCTT CCGAGTCCCT 
GAAGAAGAGA GGATGCAACA GAGGAGGGAG ATGCTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ACCCAACTAA TTTTAACCAC ATAGCACACA TGGGTCCTGG AGATGGAATA 
CAGATCCTAA AAGACCTGCC CATGAACCCA CGGCCTCAGG AAAGCCGGAC AGTATTCAGT 
GGCTCCGTCA GTATTCCCTC CATCACCAAG TCCCGCCCTG AGCCAGGCCG CTCCATGAGT 
GCCAGCAGCG GCCTGTCTGC ACGGTCGTCA GCCCAGAATG GCAGTGCACT AAAGAGGGAA 
TTCTCTGGAG GAAGCTATAA CACAAAGCGA CAGCCCATGC CCTCTCCCTC GGAGGGCTCT 
TTGTCATCAG GAGGCATGGA CCAAGGAAGT GACGCCCCTG CGAGGGACTA TGATGGAGAG 
GACTCTGATT CTCCAAGGCA TTCCACAGCT TCCAACAGCT CTAACCTGAG CAGCCCCCCA 
AGTCCCATTT CACCCCAAAA GACCAAGAGC CTCTCCCTGG AGAGCACAGA CCGCGGGAGC 
TGGGATCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346475.1
CDS	631..5622
Translation	MRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	NM_001359546.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359546.1

ORF Insert Sequence:

ATGCGATTAC ATAGAGAAGA CTTTGAAATA TTAAAGGTGA TTGGTCGAGG AGCTTTTGGG 
GAGGTTGCTG TAGTTAAACT AAAAAATGCA GATAAAGTAT TCGCCATGAA AATTCTGAAC 
AAGTGGGAAA TGCTGAAAAG AGCTGAGACA GCGTGCTTTC GTGAAGAGAG GGATGTGTTG 
GTAAATGGAG ACAGTAAGTG GATAACCACT CTGCACTATG CTTTCCAGGA TGACAATAAC 
TTATACCTGG TTATGGATTA TTATGTTGGT GGGGATTTAC TTACTCTGCT CAGCAAATTT 
GAAGATAGAT TGCCTGAAGA GATGGCTCGG TTTTACTTGG CTGAGATGGT GATAGCCATT 
GACTCAGTTC ACCAGCTCCA CTATGTTCAC AGAGACATCA AACCTGACAA TATATTGATG 
GATATGAATG GACATATTCG TTTAGCAGAT TTTGGTTCTT GTCTGAAGCT GATGGAAGAT 
GGAACGGTCC AGTCCTCGGT GGCAGTTGGA ACTCCAGATT ATATTTCTCC GGAAATCCTT 
CAGGCTATGG AAGACGGAAA AGGCAGATAT GGACCAGAGT GTGACTGGTG GTCCTTGGGG 
GTCTGTATGT ATGAGATGCT TTACGGAGAA ACACCATTCT ATGCAGAGTC TCTGGTGGAG 
ACATATGGAA AAATTATGAA TCACAAAGAG CGGTTTCAGT TTCCAGCCCA AGTGACAGAC 
GTGTCCGAAA ATGCTAAAGA TCTTATTCGA AGACTCATTT GTAGTAGAGA ACATCGACTT 
GGCCAAAATG GAATAGAAGA CTTTAAGAAG CATCCATTCT TCAGTGGAAT TGATTGGGAT 
AACATTCGGA ATTGTGAAGC ACCTTACATC CCAGAAGTTA GTAGCCCGAC AGATACGTCA 
AATTTTGATG TGGATGACGA CTGTTTAAAA AATTCTGAAA CAATGCCCCC ACCAACACAT 
ACTGCATTTT CTGGCCATCA TCTACCATTT GTTGGTTTTA CATATACTAG TAGCTGTGTT 
CTTTCTGATC GGAGCTGTTT AAGAGTCACA GCAGGCCCCA CCTCATTGGA CCTTGATGTC 
AGTGTCCAGA GGACTCTAGA CAATAACTTA GCAACTGAGG CTTATGAAAG GAGAATTAAG 
CGGCTTGAGC AGGAAAAGCT TGAACTGACT AGAAAACTTC AAGAGTCAAC ACAGACTGTT 
CAAGCTCTGC AATATTCAAC TGTTGATGGT CCACTAACGG CAAGCAAAGA TTTAGAAATT 
AAAAGTCTGA AAGAAGAGAT TGAAAAACTA AGGAAACAAG TAGCAGAAGT GAATCATTTG 
GAACAACAAC TTGAAGAAGC TAATTCTGTG AGACGAGAAC TAGATGATGC TTTCAGGCAA 
ATCAAAGCTT CTGAAAAACA AATCAAAACT TTGCAGCAGG AAAGAGAAGA ACTAAATAAG 
GAACTAGTCC AGGCTAGTGA GCGATTAAAA AACCAATCCA AAGAGCTGAA AGATGCACAC 
TGTCAGAGGA AACTGGCCAT GCAGGAATTC ATGGAGATCA ATGAGCGACT AACAGAATTG 
CACACCCAAA AGCAGAAACT TGCTCGCCAT GTCCGAGATA AGGAAGAAGA GGTGGACCTG 
GTGATGCAAA AAGCTGAGAG CTTAAGGCAG GAGCTGCGCA GAGCAGAAAG AGCCAAGAAA 
GAGCTGGAAG TTCACACCGA AGCTCTAATT GCTGAAGCAT CAAAGGACAA GAAGCTCCGA 
GAGCAGAGCG AACATTATTC TAAGCAACTG GAAAACGAGT TGGAGGGCCT GAAGCAAAAA 
CAAATTAGTT ACTCACCAGG AATATGCAGC ATAGAACATC AGCAAGAGAT AACTAAACTG 
AAAACTGACT TGGAAAAGAA AAGTATCTTT TATGAAGAAG AAATTTCTAA AAGAGAAGGA 
ATTCACGCAA GTGAGATTAA AAACCTGAAG AAGGAGCTGC ATGACTCAGA AGGCCAGCAG 
CTTGCTCTCA ACAAAGAGAT TCTGGTTTTA AAAGACAAAT TGGAAAAAAC CAGGAGAGAA 
AGTCAAAGTG AAAGGGAAGA ATTTGAAAAT GAGTTCAAAC AACAATATGA ACGGGAAAAA 
GTATTATTAA CTGAAGAGAA TAAAAAGTTA ACGAGTGAAC TCGATAAGCT GACTTCACTG 
TATGAGAGCT TAAGTTTGCG CAACCAGCAC TTAGAAGAAG AAGTCAAAGA TCTAGCAGAT 
AAGAAGGAAT CAGTTGCCCA CTGGGAAGCC CAAATCACAG AGATAATTCA GTGGGTGAGC 
GATGAGAAGG ATGCACGAGG CTATCTTCAG GCCTTAGCTT CTAAAATGAC TGAAGAGTTG 
GAAGCATTAA GAAATTCCAG CTTGGGGACA CGAGCAACAG ATATGCCCTG GAAAATGCGT 
CGTTTTGCAA AACTGGACAT GTCGGCTAGA CTAGAGTTGC AGTCTGCTCT GGATGCAGAA 
ATAAGAGCTA AGCAGGCCAT CCAGGAAGAG CTGAATAAAG TCAAAGCATC TAACATCTTA 
ACAGAATGTA AACTGAAAGA TTCAGAGAAG AAGAACTTGG AACTGTTGTC CGAAATCGAG 
CAGCTGATAA AGGACACTGA AGAGCTTAGA TCTGAAAAGG GTATAGAGCA CCAAGACTCA 
CAGCATTCTT TCTTGGCATT TTTGAATACG CCTACCGATG CTCTGGACCA ATTTGAACGC 
TCTCCGTCCT GTACTCCAGC TAGCAAAGGC AGACGTATTG CAGACTGTGC TCCACTTCCA 
GCTCACACAC CAACCTTAAG GAAAAAGGGA TGCCCGGCTT CAACTGGCTT TCCACCTAAG 
CGCAAGACTC ATCAATTTTT CGTGAAGTCT TTCACTGCCC CTACCAAGTG TCACCAGTGC 
ACCTCCTTGA TGGTCGGTTT GATAAGACAG GGCTGTTCCT GTGAAGTCTG TGGGTTCTCA 
TGTCATATAA CTTGTGTGAA CAAAGCCCCG ACGGTTTGCC CAGTTCCTCC TGAGCAAACT 
AAAGGTCCAC TGGGCATAGA CCCCCAGAAG GGAGTTGGGA CGGCGTATGA GGGTCATGTC 
AGGATCCCTA AGCCGGCGGG AGTGAAGAAA GGGTGGCAGA GAGCACTGGC TGTGGTCTGC 
GACTTCAAAC TGTTTCTTTA TGATATCGCA GAGGGAAAAG CATCGCAGCC CACCTCCGTC 
ATTAGTCAAG TGATTGACAT GAGAGATGAA GAATTTTCTG TGAGTTCAGT CTTAGCTTCT 
GATGTTATTC ACGCAAGTCG AAAAGATATC CCCTGTATAT TCAGGGTCAC AGCTTCCCAG 
CTCTCAGCAC CTAGTAACAA ATGTTCCATC CTGATGCTAG CAGACAGCGA GAATGAGAGG 
AGTAAGTGGG TGGGAGTGCT GAGTGAACTC CACAAGATTC TGAAGAAAAA CAAGTTCAGA 
GACCGCTCAG TGTATGTCCC CAAGGAGGCG TACGACAGCA CGCTACCCCT CATTAAGACA 
ACGCAGGCCG CTGCAATCAT AGATCATGAA CGGATAGCTT TGGGAAATGA AGAAGGATTA 
TTTGTTGTGC ATGTCACCAA AGATGAGATT GTTAGAGTTG GTGATAATAA GAAAATCCAT 
CAGATCGAAC TCATTCCAAG TGACCAGCTT GTCGCTGTGA TCTCAGGCCG AAATCGCCAC 
GTCCGACTTT TTCCTATGTC AGCCTTGGAT GGGCGAGAGA CGGATTTTTA TAAACTGGCA 
GAGACTAAAG GGTGTCAGAC CATAGCTGCT GGGAAAGTGC GCCACGGAGC TCTGTCCTGC 
CTGTGTGTGG CCATGAAGAG ACAGGTCCTC TGCTATGAAC TGTTCCAAAG CAAGACCAGG 
CACAGGAAGT TTAAGGAGAT TCAAGTCCCA TGTAATGTCC AGTGGATGGC CATCTTCAGT 
GAGCATCTCT GTGTGGGATT CCAGTCAGGA TTTCTCAGAT ACCCCCTGAA TGGAGAAGGA 
GGTCCATGCA ATATGCTCCA TTCAAATGAC CATACACTGT CATTCATTTC ACACCAACCA 
ATGGACGCTC TCTGTGCAGT TGAGATCTCC AACAAAGAGT ACTTGCTGTG TTTTAACAGC 
ATTGGGATAT ACACAGACTG CCAGGGCCGA AGATCCAGAC AGCAGGAACT GATGTGGCCA 
GCAAATCCTT CCTCCTGTTG TTACAATGCT CCATATCTCT CCGTGTATAG TGAAAACGCA 
GTTGATATCT TTGATGTAAA CTCCATGGAA TGGATACAGA CTCTTCCTCT CAAAAAGGTC 
CGACCTTTAA ACACTGAAGG ATCATTAAAT CTCTTAGGAT TGGAGACCAT TAGATTAATA 
TATTTCAAAA ATAAGATGGC AGAAGGGGAT GAACTTGTGG TTCCTGAAAC ATCAGATAAT 
AGTCGGAAAC AAATGGTTAG AAATATCAAC AACAAACGGC GTTACTCCTT CCGAGTCCCT 
GAAGAAGAGA GGATGCAACA GAGGAGGGAG ATGCTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ACCCAACTAA TTTTAACCAC ATAGCACACA TGGGTCCTGG AGATGGAATA 
CAGATCCTAA AAGACCTGCC CATGAACCCA CGGCCTCAGG AAAGCCGGAC AGTATTCAGT 
GGCTCCGTCA GTATTCCCTC CATCACCAAG TCCCGCCCTG AGCCAGGCCG CTCCATGAGT 
GCCAGCAGCG GCCTGTCTGC ACGGTCGTCA GCCCAGAATG GCAGTGCACT AAAGAGGGAA 
TTCTCTGGAG GAAGCTATAA CACAAAGCGA CAGCCCATGC CCTCTCCCTC GGAGGGCTCT 
TTGTCATCAG GAGGCATGGA CCAAGGAAGT GACGCCCCTG CGAGGGACTA TGATGGAGAG 
GACTCTGATT CTCCAAGGCA TTCCACAGCT TCCAACAGCT CTAACCTGAG CAGCCCCCCA 
AGTCCCATTT CACCCCAAAA GACCAAGAGC CTCTCCCTGG AGAGCACAGA CCGCGGGAGC 
TGGGATCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( NM_001359547.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346476.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 10, mRNA.

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CloneID	OMu77098
Clone ID Related Accession (Same CDS sequence)	XM_017320134.1 , NM_001359547.1
Accession Version	NM_001359547.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform 10
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC125380.4 and AC132436.3. On Jan 13, 2018 this sequence version replaced XM_017320134.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.199525.1, SRR1660813.164472.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGATG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGACTAACAG AATTGCACAC CCAAAAGCAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGCT GAGAGCTTAA GGCAGGAGCT GCGCAGAGCA 
GAAAGAGCCA AGAAAGAGCT GGAAGTTCAC ACCGAAGCTC TAATTGCTGA AGCATCAAAG 
GACAAGAAGC TCCGAGAGCA GAGCGAACAT TATTCTAAGC AACTGGAAAA CGAGTTGGAG 
GGCCTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAATAT GCAGCATAGA ACATCAGCAA 
GAGATAACTA AACTGAAAAC TGACTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAAATT 
TCTAAAAGAG AAGGAATTCA CGCAAGTGAG ATTAAAAACC TGAAGAAGGA GCTGCATGAC 
TCAGAAGGCC AGCAGCTTGC TCTCAACAAA GAGATTCTGG TTTTAAAAGA CAAATTGGAA 
AAAACCAGGA GAGAAAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAACAACAA 
TATGAACGGG AAAAAGTATT ATTAACTGAA GAGAATAAAA AGTTAACGAG TGAACTCGAT 
AAGCTGACTT CACTGTATGA GAGCTTAAGT TTGCGCAACC AGCACTTAGA AGAAGAAGTC 
AAAGATCTAG CAGATAAGAA GGAATCAGTT GCCCACTGGG AAGCCCAAAT CACAGAGATA 
ATTCAGTGGG TGAGCGATGA GAAGGATGCA CGAGGCTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AGTTGGAAGC ATTAAGAAAT TCCAGCTTGG GGACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCAAAACTG GACATGTCGG CTAGACTAGA GTTGCAGTCT 
GCTCTGGATG CAGAAATAAG AGCTAAGCAG GCCATCCAGG AAGAGCTGAA TAAAGTCAAA 
GCATCTAACA TCTTAACAGA ATGTAAACTG AAAGATTCAG AGAAGAAGAA CTTGGAACTG 
TTGTCCGAAA TCGAGCAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GACCAATTTG AAGATTCCCT TTCTTCTTCC TCATCCTCAC TGATTGATTT TTTGGATGAC 
CGCTCTCCGT CCTGTACTCC AGCTAGCAAA GGCAGACGTA TTGCAGACTG TGCTCCACTT 
CCAGCTCACA CACCAACCTT AAGGAAAAAG GGATGCCCGG CTTCAACTGG CTTTCCACCT 
AAGCGCAAGA CTCATCAATT TTTCGTGAAG TCTTTCACTG CCCCTACCAA GTGTCACCAG 
TGCACCTCCT TGATGGTCGG TTTGATAAGA CAGGGCTGTT CCTGTGAAGT CTGTGGGTTC 
TCATGTCATA TAACTTGTGT GAACAAAGCC CCGACGGTTT GCCCAGTTCC TCCTGAGCAA 
ACTAAAGGTC CACTGGGCAT AGACCCCCAG AAGGGAGTTG GGACGGCGTA TGAGGGTCAT 
GTCAGGATCC CTAAGCCGGC GGGAGTGAAG AAAGGGTGGC AGAGAGCACT GGCTGTGGTC 
TGCGACTTCA AACTGTTTCT TTATGATATC GCAGAGGGAA AAGCATCGCA GCCCACCTCC 
GTCATTAGTC AAGTGATTGA CATGAGAGAT GAAGAATTTT CTGTGAGTTC AGTCTTAGCT 
TCTGATGTTA TTCACGCAAG TCGAAAAGAT ATCCCCTGTA TATTCAGGGT CACAGCTTCC 
CAGCTCTCAG CACCTAGTAA CAAATGTTCC ATCCTGATGC TAGCAGACAG CGAGAATGAG 
AGGAGTAAGT GGGTGGGAGT GCTGAGTGAA CTCCACAAGA TTCTGAAGAA AAACAAGTTC 
AGAGACCGCT CAGTGTATGT CCCCAAGGAG GCGTACGACA GCACGCTACC CCTCATTAAG 
ACAACGCAGG CCGCTGCAAT CATAGATCAT GAACGGATAG CTTTGGGAAA TGAAGAAGGA 
TTATTTGTTG TGCATGTCAC CAAAGATGAG ATTGTTAGAG TTGGTGATAA TAAGAAAATC 
CATCAGATCG AACTCATTCC AAGTGACCAG CTTGTCGCTG TGATCTCAGG CCGAAATCGC 
CACGTCCGAC TTTTTCCTAT GTCAGCCTTG GATGGGCGAG AGACGGATTT TTATAAACTG 
GCAGAGACTA AAGGGTGTCA GACCATAGCT GCTGGGAAAG TGCGCCACGG AGCTCTGTCC 
TGCCTGTGTG TGGCCATGAA GAGACAGGTC CTCTGCTATG AACTGTTCCA AAGCAAGACC 
AGGCACAGGA AGTTTAAGGA GATTCAAGTC CCATGTAATG TCCAGTGGAT GGCCATCTTC 
AGTGAGCATC TCTGTGTGGG ATTCCAGTCA GGATTTCTCA GATACCCCCT GAATGGAGAA 
GGAGGTCCAT GCAATATGCT CCATTCAAAT GACCATACAC TGTCATTCAT TTCACACCAA 
CCAATGGACG CTCTCTGTGC AGTTGAGATC TCCAACAAAG AGTACTTGCT GTGTTTTAAC 
AGCATTGGGA TATACACAGA CTGCCAGGGC CGAAGATCCA GACAGCAGGA ACTGATGTGG 
CCAGCAAATC CTTCCTCCTG TTGTTACAAT GCTCCATATC TCTCCGTGTA TAGTGAAAAC 
GCAGTTGATA TCTTTGATGT AAACTCCATG GAATGGATAC AGACTCTTCC TCTCAAAAAG 
GTCCGACCTT TAAACACTGA AGGATCATTA AATCTCTTAG GATTGGAGAC CATTAGATTA 
ATATATTTCA AAAATAAGAT GGCAGAAGGG GATGAACTTG TGGTTCCTGA AACATCAGAT 
AATAGTCGGA AACAAATGGT TAGAAATATC AACAACAAAC GGCGTTACTC CTTCCGAGTC 
CCTGAAGAAG AGAGGATGCA ACAGAGGAGG GAGATGCTAC GAGATCCAGA AATGAGAAAT 
AAATTAATTT CTAACCCAAC TAATTTTAAC CACATAGCAC ACATGGGTCC TGGAGATGGA 
ATACAGATCC TAAAAGACCT GCCCATGAAC CCACGGCCTC AGGAAAGCCG GACAGTATTC 
AGTGGCTCCG TCAGTATTCC CTCCATCACC AAGTCCCGCC CTGAGCCAGG CCGCTCCATG 
AGTGCCAGCA GCGGCCTGTC TGCACGGTCG TCAGCCCAGA ATGGCAGTGC ACTAAAGAGG 
GAATTCTCTG GAGGAAGCTA TAACACAAAG CGACAGCCCA TGCCCTCTCC CTCGGAGGGC 
TCTTTGTCAT CAGGAGGCAT GGACCAAGGA AGTGACGCCC CTGCGAGGGA CTATGATGGA 
GAGGACTCTG ATTCTCCAAG GCATTCCACA GCTTCCAACA GCTCTAACCT GAGCAGCCCC 
CCAAGTCCCA TTTCACCCCA AAAGACCAAG AGCCTCTCCC TGGAGAGCAC AGACCGCGGG 
AGCTGGGATC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346476.1
CDS	821..5755
Translation	MKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSLSSSSSSLIDFLDDRSPSCTPASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	NM_001359547.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359547.1

ORF Insert Sequence:

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGATG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGACTAACAG AATTGCACAC CCAAAAGCAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGCT GAGAGCTTAA GGCAGGAGCT GCGCAGAGCA 
GAAAGAGCCA AGAAAGAGCT GGAAGTTCAC ACCGAAGCTC TAATTGCTGA AGCATCAAAG 
GACAAGAAGC TCCGAGAGCA GAGCGAACAT TATTCTAAGC AACTGGAAAA CGAGTTGGAG 
GGCCTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAATAT GCAGCATAGA ACATCAGCAA 
GAGATAACTA AACTGAAAAC TGACTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAAATT 
TCTAAAAGAG AAGGAATTCA CGCAAGTGAG ATTAAAAACC TGAAGAAGGA GCTGCATGAC 
TCAGAAGGCC AGCAGCTTGC TCTCAACAAA GAGATTCTGG TTTTAAAAGA CAAATTGGAA 
AAAACCAGGA GAGAAAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAACAACAA 
TATGAACGGG AAAAAGTATT ATTAACTGAA GAGAATAAAA AGTTAACGAG TGAACTCGAT 
AAGCTGACTT CACTGTATGA GAGCTTAAGT TTGCGCAACC AGCACTTAGA AGAAGAAGTC 
AAAGATCTAG CAGATAAGAA GGAATCAGTT GCCCACTGGG AAGCCCAAAT CACAGAGATA 
ATTCAGTGGG TGAGCGATGA GAAGGATGCA CGAGGCTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AGTTGGAAGC ATTAAGAAAT TCCAGCTTGG GGACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCAAAACTG GACATGTCGG CTAGACTAGA GTTGCAGTCT 
GCTCTGGATG CAGAAATAAG AGCTAAGCAG GCCATCCAGG AAGAGCTGAA TAAAGTCAAA 
GCATCTAACA TCTTAACAGA ATGTAAACTG AAAGATTCAG AGAAGAAGAA CTTGGAACTG 
TTGTCCGAAA TCGAGCAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GACCAATTTG AAGATTCCCT TTCTTCTTCC TCATCCTCAC TGATTGATTT TTTGGATGAC 
CGCTCTCCGT CCTGTACTCC AGCTAGCAAA GGCAGACGTA TTGCAGACTG TGCTCCACTT 
CCAGCTCACA CACCAACCTT AAGGAAAAAG GGATGCCCGG CTTCAACTGG CTTTCCACCT 
AAGCGCAAGA CTCATCAATT TTTCGTGAAG TCTTTCACTG CCCCTACCAA GTGTCACCAG 
TGCACCTCCT TGATGGTCGG TTTGATAAGA CAGGGCTGTT CCTGTGAAGT CTGTGGGTTC 
TCATGTCATA TAACTTGTGT GAACAAAGCC CCGACGGTTT GCCCAGTTCC TCCTGAGCAA 
ACTAAAGGTC CACTGGGCAT AGACCCCCAG AAGGGAGTTG GGACGGCGTA TGAGGGTCAT 
GTCAGGATCC CTAAGCCGGC GGGAGTGAAG AAAGGGTGGC AGAGAGCACT GGCTGTGGTC 
TGCGACTTCA AACTGTTTCT TTATGATATC GCAGAGGGAA AAGCATCGCA GCCCACCTCC 
GTCATTAGTC AAGTGATTGA CATGAGAGAT GAAGAATTTT CTGTGAGTTC AGTCTTAGCT 
TCTGATGTTA TTCACGCAAG TCGAAAAGAT ATCCCCTGTA TATTCAGGGT CACAGCTTCC 
CAGCTCTCAG CACCTAGTAA CAAATGTTCC ATCCTGATGC TAGCAGACAG CGAGAATGAG 
AGGAGTAAGT GGGTGGGAGT GCTGAGTGAA CTCCACAAGA TTCTGAAGAA AAACAAGTTC 
AGAGACCGCT CAGTGTATGT CCCCAAGGAG GCGTACGACA GCACGCTACC CCTCATTAAG 
ACAACGCAGG CCGCTGCAAT CATAGATCAT GAACGGATAG CTTTGGGAAA TGAAGAAGGA 
TTATTTGTTG TGCATGTCAC CAAAGATGAG ATTGTTAGAG TTGGTGATAA TAAGAAAATC 
CATCAGATCG AACTCATTCC AAGTGACCAG CTTGTCGCTG TGATCTCAGG CCGAAATCGC 
CACGTCCGAC TTTTTCCTAT GTCAGCCTTG GATGGGCGAG AGACGGATTT TTATAAACTG 
GCAGAGACTA AAGGGTGTCA GACCATAGCT GCTGGGAAAG TGCGCCACGG AGCTCTGTCC 
TGCCTGTGTG TGGCCATGAA GAGACAGGTC CTCTGCTATG AACTGTTCCA AAGCAAGACC 
AGGCACAGGA AGTTTAAGGA GATTCAAGTC CCATGTAATG TCCAGTGGAT GGCCATCTTC 
AGTGAGCATC TCTGTGTGGG ATTCCAGTCA GGATTTCTCA GATACCCCCT GAATGGAGAA 
GGAGGTCCAT GCAATATGCT CCATTCAAAT GACCATACAC TGTCATTCAT TTCACACCAA 
CCAATGGACG CTCTCTGTGC AGTTGAGATC TCCAACAAAG AGTACTTGCT GTGTTTTAAC 
AGCATTGGGA TATACACAGA CTGCCAGGGC CGAAGATCCA GACAGCAGGA ACTGATGTGG 
CCAGCAAATC CTTCCTCCTG TTGTTACAAT GCTCCATATC TCTCCGTGTA TAGTGAAAAC 
GCAGTTGATA TCTTTGATGT AAACTCCATG GAATGGATAC AGACTCTTCC TCTCAAAAAG 
GTCCGACCTT TAAACACTGA AGGATCATTA AATCTCTTAG GATTGGAGAC CATTAGATTA 
ATATATTTCA AAAATAAGAT GGCAGAAGGG GATGAACTTG TGGTTCCTGA AACATCAGAT 
AATAGTCGGA AACAAATGGT TAGAAATATC AACAACAAAC GGCGTTACTC CTTCCGAGTC 
CCTGAAGAAG AGAGGATGCA ACAGAGGAGG GAGATGCTAC GAGATCCAGA AATGAGAAAT 
AAATTAATTT CTAACCCAAC TAATTTTAAC CACATAGCAC ACATGGGTCC TGGAGATGGA 
ATACAGATCC TAAAAGACCT GCCCATGAAC CCACGGCCTC AGGAAAGCCG GACAGTATTC 
AGTGGCTCCG TCAGTATTCC CTCCATCACC AAGTCCCGCC CTGAGCCAGG CCGCTCCATG 
AGTGCCAGCA GCGGCCTGTC TGCACGGTCG TCAGCCCAGA ATGGCAGTGC ACTAAAGAGG 
GAATTCTCTG GAGGAAGCTA TAACACAAAG CGACAGCCCA TGCCCTCTCC CTCGGAGGGC 
TCTTTGTCAT CAGGAGGCAT GGACCAAGGA AGTGACGCCC CTGCGAGGGA CTATGATGGA 
GAGGACTCTG ATTCTCCAAG GCATTCCACA GCTTCCAACA GCTCTAACCT GAGCAGCCCC 
CCAAGTCCCA TTTCACCCCA AAAGACCAAG AGCCTCTCCC TGGAGAGCAC AGACCGCGGG 
AGCTGGGATC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( NM_001359548.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346477.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 11, mRNA.

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CloneID	OMu77099
Clone ID Related Accession (Same CDS sequence)	XM_017320142.1 , NM_001359548.1
Accession Version	NM_001359548.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform 11
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC125380.4 and AC132436.3. On Jan 13, 2018 this sequence version replaced XM_017320142.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660825.192915.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGATG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGACTAACAG AATTGCACAC CCAAAAGCAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGCT GAGAGCTTAA GGCAGGAGCT GCGCAGAGCA 
GAAAGAGCCA AGAAAGAGCT GGAAGTTCAC ACCGAAGCTC TAATTGCTGA AGCATCAAAG 
GACAAGAAGC TCCGAGAGCA GAGCGAACAT TATTCTAAGC AACTGGAAAA CGAGTTGGAG 
GGCCTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAATAT GCAGCATAGA ACATCAGCAA 
GAGATAACTA AACTGAAAAC TGACTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAAATT 
TCTAAAAGAG AAGGAATTCA CGCAAGTGAG ATTAAAAACC TGAAGAAGGA GCTGCATGAC 
TCAGAAGGCC AGCAGCTTGC TCTCAACAAA GAGATTCTGG TTTTAAAAGA CAAATTGGAA 
AAAACCAGGA GAGAAAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAACAACAA 
TATGAACGGG AAAAAGTATT ATTAACTGAA GAGAATAAAA AGTTAACGAG TGAACTCGAT 
AAGCTGACTT CACTGTATGA GAGCTTAAGT TTGCGCAACC AGCACTTAGA AGAAGAAGTC 
AAAGATCTAG CAGATAAGAA GGAATCAGTT GCCCACTGGG AAGCCCAAAT CACAGAGATA 
ATTCAGTGGG TGAGCGATGA GAAGGATGCA CGAGGCTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AGTTGGAAGC ATTAAGAAAT TCCAGCTTGG GGACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCAAAACTG GACATGTCGG CTAGACTAGA GTTGCAGTCT 
GCTCTGGATG CAGAAATAAG AGCTAAGCAG GCCATCCAGG AAGAGCTGAA TAAAGTCAAA 
GCATCTAACA TCTTAACAGA ATGTAAACTG AAAGATTCAG AGAAGAAGAA CTTGGAACTG 
TTGTCCGAAA TCGAGCAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GACCAATTTG AAGATTCCCT TTCTTCTTCC TCATCCTCAC TGATTGATTT TTTGGATGAC 
ATTGCAGACT GTGCTCCACT TCCAGCTCAC ACACCAACCT TAAGGAAAAA GGGATGCCCG 
GCTTCAACTG GCTTTCCACC TAAGCGCAAG ACTCATCAAT TTTTCGTGAA GTCTTTCACT 
GCCCCTACCA AGTGTCACCA GTGCACCTCC TTGATGGTCG GTTTGATAAG ACAGGGCTGT 
TCCTGTGAAG TCTGTGGGTT CTCATGTCAT ATAACTTGTG TGAACAAAGC CCCGACGGTT 
TGCCCAGTTC CTCCTGAGCA AACTAAAGGT CCACTGGGCA TAGACCCCCA GAAGGGAGTT 
GGGACGGCGT ATGAGGGTCA TGTCAGGATC CCTAAGCCGG CGGGAGTGAA GAAAGGGTGG 
CAGAGAGCAC TGGCTGTGGT CTGCGACTTC AAACTGTTTC TTTATGATAT CGCAGAGGGA 
AAAGCATCGC AGCCCACCTC CGTCATTAGT CAAGTGATTG ACATGAGAGA TGAAGAATTT 
TCTGTGAGTT CAGTCTTAGC TTCTGATGTT ATTCACGCAA GTCGAAAAGA TATCCCCTGT 
ATATTCAGGG TCACAGCTTC CCAGCTCTCA GCACCTAGTA ACAAATGTTC CATCCTGATG 
CTAGCAGACA GCGAGAATGA GAGGAGTAAG TGGGTGGGAG TGCTGAGTGA ACTCCACAAG 
ATTCTGAAGA AAAACAAGTT CAGAGACCGC TCAGTGTATG TCCCCAAGGA GGCGTACGAC 
AGCACGCTAC CCCTCATTAA GACAACGCAG GCCGCTGCAA TCATAGATCA TGAACGGATA 
GCTTTGGGAA ATGAAGAAGG ATTATTTGTT GTGCATGTCA CCAAAGATGA GATTGTTAGA 
GTTGGTGATA ATAAGAAAAT CCATCAGATC GAACTCATTC CAAGTGACCA GCTTGTCGCT 
GTGATCTCAG GCCGAAATCG CCACGTCCGA CTTTTTCCTA TGTCAGCCTT GGATGGGCGA 
GAGACGGATT TTTATAAACT GGCAGAGACT AAAGGGTGTC AGACCATAGC TGCTGGGAAA 
GTGCGCCACG GAGCTCTGTC CTGCCTGTGT GTGGCCATGA AGAGACAGGT CCTCTGCTAT 
GAACTGTTCC AAAGCAAGAC CAGGCACAGG AAGTTTAAGG AGATTCAAGT CCCATGTAAT 
GTCCAGTGGA TGGCCATCTT CAGTGAGCAT CTCTGTGTGG GATTCCAGTC AGGATTTCTC 
AGATACCCCC TGAATGGAGA AGGAGGTCCA TGCAATATGC TCCATTCAAA TGACCATACA 
CTGTCATTCA TTTCACACCA ACCAATGGAC GCTCTCTGTG CAGTTGAGAT CTCCAACAAA 
GAGTACTTGC TGTGTTTTAA CAGCATTGGG ATATACACAG ACTGCCAGGG CCGAAGATCC 
AGACAGCAGG AACTGATGTG GCCAGCAAAT CCTTCCTCCT GTTGTTACAA TGCTCCATAT 
CTCTCCGTGT ATAGTGAAAA CGCAGTTGAT ATCTTTGATG TAAACTCCAT GGAATGGATA 
CAGACTCTTC CTCTCAAAAA GGTCCGACCT TTAAACACTG AAGGATCATT AAATCTCTTA 
GGATTGGAGA CCATTAGATT AATATATTTC AAAAATAAGA TGGCAGAAGG GGATGAACTT 
GTGGTTCCTG AAACATCAGA TAATAGTCGG AAACAAATGG TTAGAAATAT CAACAACAAA 
CGGCGTTACT CCTTCCGAGT CCCTGAAGAA GAGAGGATGC AACAGAGGAG GGAGATGCTA 
CGAGATCCAG AAATGAGAAA TAAATTAATT TCTAACCCAA CTAATTTTAA CCACATAGCA 
CACATGGGTC CTGGAGATGG AATACAGATC CTAAAAGACC TGCCCATGAA CCCACGGCCT 
CAGGAAAGCC GGACAGTATT CAGTGGCTCC GTCAGTATTC CCTCCATCAC CAAGTCCCGC 
CCTGAGCCAG GCCGCTCCAT GAGTGCCAGC AGCGGCCTGT CTGCACGGTC GTCAGCCCAG 
AATGGCAGTG CACTAAAGAG GGAATTCTCT GGAGGAAGCT ATAACACAAA GCGACAGCCC 
ATGCCCTCTC CCTCGGAGGG CTCTTTGTCA TCAGGAGGCA TGGACCAAGG AAGTGACGCC 
CCTGCGAGGG ACTATGATGG AGAGGACTCT GATTCTCCAA GGCATTCCAC AGCTTCCAAC 
AGCTCTAACC TGAGCAGCCC CCCAAGTCCC ATTTCACCCC AAAAGACCAA GAGCCTCTCC 
CTGGAGAGCA CAGACCGCGG GAGCTGGGAT CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346477.1
CDS	821..5716
Translation	MKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSLSSSSSSLIDFLDDIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	NM_001359548.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359548.1

ORF Insert Sequence:

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGATG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGACTAACAG AATTGCACAC CCAAAAGCAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGCT GAGAGCTTAA GGCAGGAGCT GCGCAGAGCA 
GAAAGAGCCA AGAAAGAGCT GGAAGTTCAC ACCGAAGCTC TAATTGCTGA AGCATCAAAG 
GACAAGAAGC TCCGAGAGCA GAGCGAACAT TATTCTAAGC AACTGGAAAA CGAGTTGGAG 
GGCCTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAATAT GCAGCATAGA ACATCAGCAA 
GAGATAACTA AACTGAAAAC TGACTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAAATT 
TCTAAAAGAG AAGGAATTCA CGCAAGTGAG ATTAAAAACC TGAAGAAGGA GCTGCATGAC 
TCAGAAGGCC AGCAGCTTGC TCTCAACAAA GAGATTCTGG TTTTAAAAGA CAAATTGGAA 
AAAACCAGGA GAGAAAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAACAACAA 
TATGAACGGG AAAAAGTATT ATTAACTGAA GAGAATAAAA AGTTAACGAG TGAACTCGAT 
AAGCTGACTT CACTGTATGA GAGCTTAAGT TTGCGCAACC AGCACTTAGA AGAAGAAGTC 
AAAGATCTAG CAGATAAGAA GGAATCAGTT GCCCACTGGG AAGCCCAAAT CACAGAGATA 
ATTCAGTGGG TGAGCGATGA GAAGGATGCA CGAGGCTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AGTTGGAAGC ATTAAGAAAT TCCAGCTTGG GGACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCAAAACTG GACATGTCGG CTAGACTAGA GTTGCAGTCT 
GCTCTGGATG CAGAAATAAG AGCTAAGCAG GCCATCCAGG AAGAGCTGAA TAAAGTCAAA 
GCATCTAACA TCTTAACAGA ATGTAAACTG AAAGATTCAG AGAAGAAGAA CTTGGAACTG 
TTGTCCGAAA TCGAGCAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GACCAATTTG AAGATTCCCT TTCTTCTTCC TCATCCTCAC TGATTGATTT TTTGGATGAC 
ATTGCAGACT GTGCTCCACT TCCAGCTCAC ACACCAACCT TAAGGAAAAA GGGATGCCCG 
GCTTCAACTG GCTTTCCACC TAAGCGCAAG ACTCATCAAT TTTTCGTGAA GTCTTTCACT 
GCCCCTACCA AGTGTCACCA GTGCACCTCC TTGATGGTCG GTTTGATAAG ACAGGGCTGT 
TCCTGTGAAG TCTGTGGGTT CTCATGTCAT ATAACTTGTG TGAACAAAGC CCCGACGGTT 
TGCCCAGTTC CTCCTGAGCA AACTAAAGGT CCACTGGGCA TAGACCCCCA GAAGGGAGTT 
GGGACGGCGT ATGAGGGTCA TGTCAGGATC CCTAAGCCGG CGGGAGTGAA GAAAGGGTGG 
CAGAGAGCAC TGGCTGTGGT CTGCGACTTC AAACTGTTTC TTTATGATAT CGCAGAGGGA 
AAAGCATCGC AGCCCACCTC CGTCATTAGT CAAGTGATTG ACATGAGAGA TGAAGAATTT 
TCTGTGAGTT CAGTCTTAGC TTCTGATGTT ATTCACGCAA GTCGAAAAGA TATCCCCTGT 
ATATTCAGGG TCACAGCTTC CCAGCTCTCA GCACCTAGTA ACAAATGTTC CATCCTGATG 
CTAGCAGACA GCGAGAATGA GAGGAGTAAG TGGGTGGGAG TGCTGAGTGA ACTCCACAAG 
ATTCTGAAGA AAAACAAGTT CAGAGACCGC TCAGTGTATG TCCCCAAGGA GGCGTACGAC 
AGCACGCTAC CCCTCATTAA GACAACGCAG GCCGCTGCAA TCATAGATCA TGAACGGATA 
GCTTTGGGAA ATGAAGAAGG ATTATTTGTT GTGCATGTCA CCAAAGATGA GATTGTTAGA 
GTTGGTGATA ATAAGAAAAT CCATCAGATC GAACTCATTC CAAGTGACCA GCTTGTCGCT 
GTGATCTCAG GCCGAAATCG CCACGTCCGA CTTTTTCCTA TGTCAGCCTT GGATGGGCGA 
GAGACGGATT TTTATAAACT GGCAGAGACT AAAGGGTGTC AGACCATAGC TGCTGGGAAA 
GTGCGCCACG GAGCTCTGTC CTGCCTGTGT GTGGCCATGA AGAGACAGGT CCTCTGCTAT 
GAACTGTTCC AAAGCAAGAC CAGGCACAGG AAGTTTAAGG AGATTCAAGT CCCATGTAAT 
GTCCAGTGGA TGGCCATCTT CAGTGAGCAT CTCTGTGTGG GATTCCAGTC AGGATTTCTC 
AGATACCCCC TGAATGGAGA AGGAGGTCCA TGCAATATGC TCCATTCAAA TGACCATACA 
CTGTCATTCA TTTCACACCA ACCAATGGAC GCTCTCTGTG CAGTTGAGAT CTCCAACAAA 
GAGTACTTGC TGTGTTTTAA CAGCATTGGG ATATACACAG ACTGCCAGGG CCGAAGATCC 
AGACAGCAGG AACTGATGTG GCCAGCAAAT CCTTCCTCCT GTTGTTACAA TGCTCCATAT 
CTCTCCGTGT ATAGTGAAAA CGCAGTTGAT ATCTTTGATG TAAACTCCAT GGAATGGATA 
CAGACTCTTC CTCTCAAAAA GGTCCGACCT TTAAACACTG AAGGATCATT AAATCTCTTA 
GGATTGGAGA CCATTAGATT AATATATTTC AAAAATAAGA TGGCAGAAGG GGATGAACTT 
GTGGTTCCTG AAACATCAGA TAATAGTCGG AAACAAATGG TTAGAAATAT CAACAACAAA 
CGGCGTTACT CCTTCCGAGT CCCTGAAGAA GAGAGGATGC AACAGAGGAG GGAGATGCTA 
CGAGATCCAG AAATGAGAAA TAAATTAATT TCTAACCCAA CTAATTTTAA CCACATAGCA 
CACATGGGTC CTGGAGATGG AATACAGATC CTAAAAGACC TGCCCATGAA CCCACGGCCT 
CAGGAAAGCC GGACAGTATT CAGTGGCTCC GTCAGTATTC CCTCCATCAC CAAGTCCCGC 
CCTGAGCCAG GCCGCTCCAT GAGTGCCAGC AGCGGCCTGT CTGCACGGTC GTCAGCCCAG 
AATGGCAGTG CACTAAAGAG GGAATTCTCT GGAGGAAGCT ATAACACAAA GCGACAGCCC 
ATGCCCTCTC CCTCGGAGGG CTCTTTGTCA TCAGGAGGCA TGGACCAAGG AAGTGACGCC 
CCTGCGAGGG ACTATGATGG AGAGGACTCT GATTCTCCAA GGCATTCCAC AGCTTCCAAC 
AGCTCTAACC TGAGCAGCCC CCCAAGTCCC ATTTCACCCC AAAAGACCAA GAGCCTCTCC 
CTGGAGAGCA CAGACCGCGG GAGCTGGGAT CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( NM_001359549.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346478.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 12, mRNA.

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CloneID	OMu77101
Clone ID Related Accession (Same CDS sequence)	NM_001359549.1 , XM_017320146.1
Accession Version	NM_001359549.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform 12
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC125380.4 and AC132436.3. On Jan 13, 2018 this sequence version replaced XM_017320146.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.203766.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGATG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGACTAACAG AATTGCACAC CCAAAAGCAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGCT GAGAGCTTAA GGCAGGAGCT GCGCAGAGCA 
GAAAGAGCCA AGAAAGAGCT GGAAGTTCAC ACCGAAGCTC TAATTGCTGA AGCATCAAAG 
GACAAGAAGC TCCGAGAGCA GAGCGAACAT TATTCTAAGC AACTGGAAAA CGAGTTGGAG 
GGCCTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAATAT GCAGCATAGA ACATCAGCAA 
GAGATAACTA AACTGAAAAC TGACTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAAATT 
TCTAAAAGAG AAGGAATTCA CGCAAGTGAG ATTAAAAACC TGAAGAAGGA GCTGCATGAC 
TCAGAAGGCC AGCAGCTTGC TCTCAACAAA GAGATTCTGG TTTTAAAAGA CAAATTGGAA 
AAAACCAGGA GAGAAAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAACAACAA 
TATGAACGGG AAAAAGTATT ATTAACTGAA GAGAATAAAA AGTTAACGAG TGAACTCGAT 
AAGCTGACTT CACTGTATGA GAGCTTAAGT TTGCGCAACC AGCACTTAGA AGAAGAAGTC 
AAAGATCTAG CAGATAAGAA GGAATCAGTT GCCCACTGGG AAGCCCAAAT CACAGAGATA 
ATTCAGTGGG TGAGCGATGA GAAGGATGCA CGAGGCTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AGTTGGAAGC ATTAAGAAAT TCCAGCTTGG GGACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCAAAACTG GACATGTCGG CTAGACTAGA GTTGCAGTCT 
GCTCTGGATG CAGAAATAAG AGCTAAGCAG GCCATCCAGG AAGAGCTGAA TAAAGTCAAA 
GCATCTAACA TCTTAACAGA ATGTAAACTG AAAGATTCAG AGAAGAAGAA CTTGGAACTG 
TTGTCCGAAA TCGAGCAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGCTAGC 
AAAGGCAGAC GTATTGCAGA CTGTGCTCCA CTTCCAGCTC ACACACCAAC CTTAAGGAAA 
AAGGGATGCC CGGCTTCAAC TGGCTTTCCA CCTAAGCGCA AGACTCATCA ATTTTTCGTG 
AAGTCTTTCA CTGCCCCTAC CAAGTGTCAC CAGTGCACCT CCTTGATGGT CGGTTTGATA 
AGACAGGGCT GTTCCTGTGA AGTCTGTGGG TTCTCATGTC ATATAACTTG TGTGAACAAA 
GCCCCGACGG TTTGCCCAGT TCCTCCTGAG CAAACTAAAG GTCCACTGGG CATAGACCCC 
CAGAAGGGAG TTGGGACGGC GTATGAGGGT CATGTCAGGA TCCCTAAGCC GGCGGGAGTG 
AAGAAAGGGT GGCAGAGAGC ACTGGCTGTG GTCTGCGACT TCAAACTGTT TCTTTATGAT 
ATCGCAGAGG GAAAAGCATC GCAGCCCACC TCCGTCATTA GTCAAGTGAT TGACATGAGA 
GATGAAGAAT TTTCTGTGAG TTCAGTCTTA GCTTCTGATG TTATTCACGC AAGTCGAAAA 
GATATCCCCT GTATATTCAG GGTCACAGCT TCCCAGCTCT CAGCACCTAG TAACAAATGT 
TCCATCCTGA TGCTAGCAGA CAGCGAGAAT GAGAGGAGTA AGTGGGTGGG AGTGCTGAGT 
GAACTCCACA AGATTCTGAA GAAAAACAAG TTCAGAGACC GCTCAGTGTA TGTCCCCAAG 
GAGGCGTACG ACAGCACGCT ACCCCTCATT AAGACAACGC AGGCCGCTGC AATCATAGAT 
CATGAACGGA TAGCTTTGGG AAATGAAGAA GGATTATTTG TTGTGCATGT CACCAAAGAT 
GAGATTGTTA GAGTTGGTGA TAATAAGAAA ATCCATCAGA TCGAACTCAT TCCAAGTGAC 
CAGCTTGTCG CTGTGATCTC AGGCCGAAAT CGCCACGTCC GACTTTTTCC TATGTCAGCC 
TTGGATGGGC GAGAGACGGA TTTTTATAAA CTGGCAGAGA CTAAAGGGTG TCAGACCATA 
GCTGCTGGGA AAGTGCGCCA CGGAGCTCTG TCCTGCCTGT GTGTGGCCAT GAAGAGACAG 
GTCCTCTGCT ATGAACTGTT CCAAAGCAAG ACCAGGCACA GGAAGTTTAA GGAGATTCAA 
GTCCCATGTA ATGTCCAGTG GATGGCCATC TTCAGTGAGC ATCTCTGTGT GGGATTCCAG 
TCAGGATTTC TCAGATACCC CCTGAATGGA GAAGGAGGTC CATGCAATAT GCTCCATTCA 
AATGACCATA CACTGTCATT CATTTCACAC CAACCAATGG ACGCTCTCTG TGCAGTTGAG 
ATCTCCAACA AAGAGTACTT GCTGTGTTTT AACAGCATTG GGATATACAC AGACTGCCAG 
GGCCGAAGAT CCAGACAGCA GGAACTGATG TGGCCAGCAA ATCCTTCCTC CTGTTGTTAC 
AATGCTCCAT ATCTCTCCGT GTATAGTGAA AACGCAGTTG ATATCTTTGA TGTAAACTCC 
ATGGAATGGA TACAGACTCT TCCTCTCAAA AAGGTCCGAC CTTTAAACAC TGAAGGATCA 
TTAAATCTCT TAGGATTGGA GACCATTAGA TTAATATATT TCAAAAATAA GATGGCAGAA 
GGGGATGAAC TTGTGGTTCC TGAAACATCA GATAATAGTC GGAAACAAAT GGTTAGAAAT 
ATCAACAACA AACGGCGTTA CTCCTTCCGA GTCCCTGAAG AAGAGAGGAT GCAACAGAGG 
AGGGAGATGC TACGAGATCC AGAAATGAGA AATAAATTAA TTTCTAACCC AACTAATTTT 
AACCACATAG CACACATGGG TCCTGGAGAT GGAATACAGA TCCTAAAAGA CCTGCCCATG 
AACCCACGGC CTCAGGAAAG CCGGACAGTA TTCAGTGGCT CCGTCAGTAT TCCCTCCATC 
ACCAAGTCCC GCCCTGAGCC AGGCCGCTCC ATGAGTGCCA GCAGCGGCCT GTCTGCACGG 
TCGTCAGCCC AGAATGGCAG TGCACTAAAG AGGGAATTCT CTGGAGGAAG CTATAACACA 
AAGCGACAGC CCATGCCCTC TCCCTCGGAG GGCTCTTTGT CATCAGGAGG CATGGACCAA 
GGAAGTGACG CCCCTGCGAG GGACTATGAT GGAGAGGACT CTGATTCTCC AAGGCATTCC 
ACAGCTTCCA ACAGCTCTAA CCTGAGCAGC CCCCCAAGTC CCATTTCACC CCAAAAGACC 
AAGAGCCTCT CCCTGGAGAG CACAGACCGC GGGAGCTGGG ATCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346478.1
CDS	821..5608
Translation	MKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	NM_001359549.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359549.1

ORF Insert Sequence:

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGATG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGACTAACAG AATTGCACAC CCAAAAGCAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGCT GAGAGCTTAA GGCAGGAGCT GCGCAGAGCA 
GAAAGAGCCA AGAAAGAGCT GGAAGTTCAC ACCGAAGCTC TAATTGCTGA AGCATCAAAG 
GACAAGAAGC TCCGAGAGCA GAGCGAACAT TATTCTAAGC AACTGGAAAA CGAGTTGGAG 
GGCCTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAATAT GCAGCATAGA ACATCAGCAA 
GAGATAACTA AACTGAAAAC TGACTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAAATT 
TCTAAAAGAG AAGGAATTCA CGCAAGTGAG ATTAAAAACC TGAAGAAGGA GCTGCATGAC 
TCAGAAGGCC AGCAGCTTGC TCTCAACAAA GAGATTCTGG TTTTAAAAGA CAAATTGGAA 
AAAACCAGGA GAGAAAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAACAACAA 
TATGAACGGG AAAAAGTATT ATTAACTGAA GAGAATAAAA AGTTAACGAG TGAACTCGAT 
AAGCTGACTT CACTGTATGA GAGCTTAAGT TTGCGCAACC AGCACTTAGA AGAAGAAGTC 
AAAGATCTAG CAGATAAGAA GGAATCAGTT GCCCACTGGG AAGCCCAAAT CACAGAGATA 
ATTCAGTGGG TGAGCGATGA GAAGGATGCA CGAGGCTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AGTTGGAAGC ATTAAGAAAT TCCAGCTTGG GGACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCAAAACTG GACATGTCGG CTAGACTAGA GTTGCAGTCT 
GCTCTGGATG CAGAAATAAG AGCTAAGCAG GCCATCCAGG AAGAGCTGAA TAAAGTCAAA 
GCATCTAACA TCTTAACAGA ATGTAAACTG AAAGATTCAG AGAAGAAGAA CTTGGAACTG 
TTGTCCGAAA TCGAGCAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGCTAGC 
AAAGGCAGAC GTATTGCAGA CTGTGCTCCA CTTCCAGCTC ACACACCAAC CTTAAGGAAA 
AAGGGATGCC CGGCTTCAAC TGGCTTTCCA CCTAAGCGCA AGACTCATCA ATTTTTCGTG 
AAGTCTTTCA CTGCCCCTAC CAAGTGTCAC CAGTGCACCT CCTTGATGGT CGGTTTGATA 
AGACAGGGCT GTTCCTGTGA AGTCTGTGGG TTCTCATGTC ATATAACTTG TGTGAACAAA 
GCCCCGACGG TTTGCCCAGT TCCTCCTGAG CAAACTAAAG GTCCACTGGG CATAGACCCC 
CAGAAGGGAG TTGGGACGGC GTATGAGGGT CATGTCAGGA TCCCTAAGCC GGCGGGAGTG 
AAGAAAGGGT GGCAGAGAGC ACTGGCTGTG GTCTGCGACT TCAAACTGTT TCTTTATGAT 
ATCGCAGAGG GAAAAGCATC GCAGCCCACC TCCGTCATTA GTCAAGTGAT TGACATGAGA 
GATGAAGAAT TTTCTGTGAG TTCAGTCTTA GCTTCTGATG TTATTCACGC AAGTCGAAAA 
GATATCCCCT GTATATTCAG GGTCACAGCT TCCCAGCTCT CAGCACCTAG TAACAAATGT 
TCCATCCTGA TGCTAGCAGA CAGCGAGAAT GAGAGGAGTA AGTGGGTGGG AGTGCTGAGT 
GAACTCCACA AGATTCTGAA GAAAAACAAG TTCAGAGACC GCTCAGTGTA TGTCCCCAAG 
GAGGCGTACG ACAGCACGCT ACCCCTCATT AAGACAACGC AGGCCGCTGC AATCATAGAT 
CATGAACGGA TAGCTTTGGG AAATGAAGAA GGATTATTTG TTGTGCATGT CACCAAAGAT 
GAGATTGTTA GAGTTGGTGA TAATAAGAAA ATCCATCAGA TCGAACTCAT TCCAAGTGAC 
CAGCTTGTCG CTGTGATCTC AGGCCGAAAT CGCCACGTCC GACTTTTTCC TATGTCAGCC 
TTGGATGGGC GAGAGACGGA TTTTTATAAA CTGGCAGAGA CTAAAGGGTG TCAGACCATA 
GCTGCTGGGA AAGTGCGCCA CGGAGCTCTG TCCTGCCTGT GTGTGGCCAT GAAGAGACAG 
GTCCTCTGCT ATGAACTGTT CCAAAGCAAG ACCAGGCACA GGAAGTTTAA GGAGATTCAA 
GTCCCATGTA ATGTCCAGTG GATGGCCATC TTCAGTGAGC ATCTCTGTGT GGGATTCCAG 
TCAGGATTTC TCAGATACCC CCTGAATGGA GAAGGAGGTC CATGCAATAT GCTCCATTCA 
AATGACCATA CACTGTCATT CATTTCACAC CAACCAATGG ACGCTCTCTG TGCAGTTGAG 
ATCTCCAACA AAGAGTACTT GCTGTGTTTT AACAGCATTG GGATATACAC AGACTGCCAG 
GGCCGAAGAT CCAGACAGCA GGAACTGATG TGGCCAGCAA ATCCTTCCTC CTGTTGTTAC 
AATGCTCCAT ATCTCTCCGT GTATAGTGAA AACGCAGTTG ATATCTTTGA TGTAAACTCC 
ATGGAATGGA TACAGACTCT TCCTCTCAAA AAGGTCCGAC CTTTAAACAC TGAAGGATCA 
TTAAATCTCT TAGGATTGGA GACCATTAGA TTAATATATT TCAAAAATAA GATGGCAGAA 
GGGGATGAAC TTGTGGTTCC TGAAACATCA GATAATAGTC GGAAACAAAT GGTTAGAAAT 
ATCAACAACA AACGGCGTTA CTCCTTCCGA GTCCCTGAAG AAGAGAGGAT GCAACAGAGG 
AGGGAGATGC TACGAGATCC AGAAATGAGA AATAAATTAA TTTCTAACCC AACTAATTTT 
AACCACATAG CACACATGGG TCCTGGAGAT GGAATACAGA TCCTAAAAGA CCTGCCCATG 
AACCCACGGC CTCAGGAAAG CCGGACAGTA TTCAGTGGCT CCGTCAGTAT TCCCTCCATC 
ACCAAGTCCC GCCCTGAGCC AGGCCGCTCC ATGAGTGCCA GCAGCGGCCT GTCTGCACGG 
TCGTCAGCCC AGAATGGCAG TGCACTAAAG AGGGAATTCT CTGGAGGAAG CTATAACACA 
AAGCGACAGC CCATGCCCTC TCCCTCGGAG GGCTCTTTGT CATCAGGAGG CATGGACCAA 
GGAAGTGACG CCCCTGCGAG GGACTATGAT GGAGAGGACT CTGATTCTCC AAGGCATTCC 
ACAGCTTCCA ACAGCTCTAA CCTGAGCAGC CCCCCAAGTC CCATTTCACC CCAAAAGACC 
AAGAGCCTCT CCCTGGAGAG CACAGACCGC GGGAGCTGGG ATCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_017320119.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175608.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu28721
Clone ID Related Accession (Same CDS sequence)	XM_017320119.2 , XM_017320119.1 , XM_006496801.3
Accession Version	XM_017320119.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320119.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAAGATTCC CTTTCTTCTT CCTCATCCTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC GTCCTGTACT CCAGCTAGCA AAGGCAGACG TATTGCAGAC 
TGTGCTCCAC TTCCAGCTCA CACACCAACC TTAAGGAAAA AGGGATGCCC GGCTTCAACT 
GGCTTTCCAC CTAAGCGCAA GACTCATCAA TTTTTCGTGA AGTCTTTCAC TGCCCCTACC 
AAGTGTCACC AGTGCACCTC CTTGATGGTC GGTTTGATAA GACAGGGCTG TTCCTGTGAA 
GTCTGTGGGT TCTCATGTCA TATAACTTGT GTGAACAAAG CCCCGACGGT TTGCCCAGTT 
CCTCCTGAGC AAACTAAAGG TCCACTGGGC ATAGACCCCC AGAAGGGAGT TGGGACGGCG 
TATGAGGGTC ATGTCAGGAT CCCTAAGCCG GCGGGAGTGA AGAAAGGGTG GCAGAGAGCA 
CTGGCTGTGG TCTGCGACTT CAAACTGTTT CTTTATGATA TCGCAGAGGG AAAAGCATCG 
CAGCCCACCT CCGTCATTAG TCAAGTGATT GACATGAGAG ATGAAGAATT TTCTGTGAGT 
TCAGTCTTAG CTTCTGATGT TATTCACGCA AGTCGAAAAG ATATCCCCTG TATATTCAGG 
GTCACAGCTT CCCAGCTCTC AGCACCTAGT AACAAATGTT CCATCCTGAT GCTAGCAGAC 
AGCGAGAATG AGAGGAGTAA GTGGGTGGGA GTGCTGAGTG AACTCCACAA GATTCTGAAG 
AAAAACAAGT TCAGAGACCG CTCAGTGTAT GTCCCCAAGG AGGCGTACGA CAGCACGCTA 
CCCCTCATTA AGACAACGCA GGCCGCTGCA ATCATAGATC ATGAACGGAT AGCTTTGGGA 
AATGAAGAAG GATTATTTGT TGTGCATGTC ACCAAAGATG AGATTGTTAG AGTTGGTGAT 
AATAAGAAAA TCCATCAGAT CGAACTCATT CCAAGTGACC AGCTTGTCGC TGTGATCTCA 
GGCCGAAATC GCCACGTCCG ACTTTTTCCT ATGTCAGCCT TGGATGGGCG AGAGACGGAT 
TTTTATAAAC TGGCAGAGAC TAAAGGGTGT CAGACCATAG CTGCTGGGAA AGTGCGCCAC 
GGAGCTCTGT CCTGCCTGTG TGTGGCCATG AAGAGACAGG TCCTCTGCTA TGAACTGTTC 
CAAAGCAAGA CCAGGCACAG GAAGTTTAAG GAGATTCAAG TCCCATGTAA TGTCCAGTGG 
ATGGCCATCT TCAGTGAGCA TCTCTGTGTG GGATTCCAGT CAGGATTTCT CAGATACCCC 
CTGAATGGAG AAGGAGGTCC ATGCAATATG CTCCATTCAA ATGACCATAC ACTGTCATTC 
ATTTCACACC AACCAATGGA CGCTCTCTGT GCAGTTGAGA TCTCCAACAA AGAGTACTTG 
CTGTGTTTTA ACAGCATTGG GATATACACA GACTGCCAGG GCCGAAGATC CAGACAGCAG 
GAACTGATGT GGCCAGCAAA TCCTTCCTCC TGTTGTTACA ATGCTCCATA TCTCTCCGTG 
TATAGTGAAA ACGCAGTTGA TATCTTTGAT GTAAACTCCA TGGAATGGAT ACAGACTCTT 
CCTCTCAAAA AGGTCCGACC TTTAAACACT GAAGGATCAT TAAATCTCTT AGGATTGGAG 
ACCATTAGAT TAATATATTT CAAAAATAAG ATGGCAGAAG GGGATGAACT TGTGGTTCCT 
GAAACATCAG ATAATAGTCG GAAACAAATG GTTAGAAATA TCAACAACAA ACGGCGTTAC 
TCCTTCCGAG TCCCTGAAGA AGAGAGGATG CAACAGAGGA GGGAGATGCT ACGAGATCCA 
GAAATGAGAA ATAAATTAAT TTCTAACCCA ACTAATTTTA ACCACATAGC ACACATGGGT 
CCTGGAGATG GAATACAGAT CCTAAAAGAC CTGCCCATGC CAGGTTTCCC TTATTCACCC 
CCTCATCACC ACTCCGGCCT GATCTCCTCT CCGAGCAACT TTGAGCACAT CTATCACATG 
ACTGTCACTT CTGCTGAACA GTTTCTCTCT CCTGAGTCCA TAAGCCCTGG GTCCCCCCCG 
TCCCTGCGCT CTGCCCCTGG CACACCACAC TCTGTGACTC TGAGGAACCC ACGGCCTCAG 
GAAAGCCGGA CAGTATTCAG TGGCTCCGTC AGTATTCCCT CCATCACCAA GTCCCGCCCT 
GAGCCAGGCC GCTCCATGAG TGCCAGCAGC GGCCTGTCTG CACGGTCGTC AGCCCAGAAT 
GGCAGTGCAC TAAAGAGGGA ATTCTCTGGA GGAAGCTATA ACACAAAGCG ACAGCCCATG 
CCCTCTCCCT CGGAGGGCTC TTTGTCATCA GGAGGCATGG ACCAAGGAAG TGACGCCCCT 
GCGAGGGACT ATGATGGAGA GGACTCTGAT TCTCCAAGGC ATTCCACAGC TTCCAACAGC 
TCTAACCTGA GCAGCCCCCC AAGTCCCATT TCACCCCAAA AGACCAAGAG CCTCTCCCTG 
GAGAGCACAG ACCGCGGGAG CTGGGATCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175608.1
CDS	1032..6464
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSLSSSSSSLIDFLDDRSPSCTPASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_017320119.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320119.2

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAAGATTCC CTTTCTTCTT CCTCATCCTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC GTCCTGTACT CCAGCTAGCA AAGGCAGACG TATTGCAGAC 
TGTGCTCCAC TTCCAGCTCA CACACCAACC TTAAGGAAAA AGGGATGCCC GGCTTCAACT 
GGCTTTCCAC CTAAGCGCAA GACTCATCAA TTTTTCGTGA AGTCTTTCAC TGCCCCTACC 
AAGTGTCACC AGTGCACCTC CTTGATGGTC GGTTTGATAA GACAGGGCTG TTCCTGTGAA 
GTCTGTGGGT TCTCATGTCA TATAACTTGT GTGAACAAAG CCCCGACGGT TTGCCCAGTT 
CCTCCTGAGC AAACTAAAGG TCCACTGGGC ATAGACCCCC AGAAGGGAGT TGGGACGGCG 
TATGAGGGTC ATGTCAGGAT CCCTAAGCCG GCGGGAGTGA AGAAAGGGTG GCAGAGAGCA 
CTGGCTGTGG TCTGCGACTT CAAACTGTTT CTTTATGATA TCGCAGAGGG AAAAGCATCG 
CAGCCCACCT CCGTCATTAG TCAAGTGATT GACATGAGAG ATGAAGAATT TTCTGTGAGT 
TCAGTCTTAG CTTCTGATGT TATTCACGCA AGTCGAAAAG ATATCCCCTG TATATTCAGG 
GTCACAGCTT CCCAGCTCTC AGCACCTAGT AACAAATGTT CCATCCTGAT GCTAGCAGAC 
AGCGAGAATG AGAGGAGTAA GTGGGTGGGA GTGCTGAGTG AACTCCACAA GATTCTGAAG 
AAAAACAAGT TCAGAGACCG CTCAGTGTAT GTCCCCAAGG AGGCGTACGA CAGCACGCTA 
CCCCTCATTA AGACAACGCA GGCCGCTGCA ATCATAGATC ATGAACGGAT AGCTTTGGGA 
AATGAAGAAG GATTATTTGT TGTGCATGTC ACCAAAGATG AGATTGTTAG AGTTGGTGAT 
AATAAGAAAA TCCATCAGAT CGAACTCATT CCAAGTGACC AGCTTGTCGC TGTGATCTCA 
GGCCGAAATC GCCACGTCCG ACTTTTTCCT ATGTCAGCCT TGGATGGGCG AGAGACGGAT 
TTTTATAAAC TGGCAGAGAC TAAAGGGTGT CAGACCATAG CTGCTGGGAA AGTGCGCCAC 
GGAGCTCTGT CCTGCCTGTG TGTGGCCATG AAGAGACAGG TCCTCTGCTA TGAACTGTTC 
CAAAGCAAGA CCAGGCACAG GAAGTTTAAG GAGATTCAAG TCCCATGTAA TGTCCAGTGG 
ATGGCCATCT TCAGTGAGCA TCTCTGTGTG GGATTCCAGT CAGGATTTCT CAGATACCCC 
CTGAATGGAG AAGGAGGTCC ATGCAATATG CTCCATTCAA ATGACCATAC ACTGTCATTC 
ATTTCACACC AACCAATGGA CGCTCTCTGT GCAGTTGAGA TCTCCAACAA AGAGTACTTG 
CTGTGTTTTA ACAGCATTGG GATATACACA GACTGCCAGG GCCGAAGATC CAGACAGCAG 
GAACTGATGT GGCCAGCAAA TCCTTCCTCC TGTTGTTACA ATGCTCCATA TCTCTCCGTG 
TATAGTGAAA ACGCAGTTGA TATCTTTGAT GTAAACTCCA TGGAATGGAT ACAGACTCTT 
CCTCTCAAAA AGGTCCGACC TTTAAACACT GAAGGATCAT TAAATCTCTT AGGATTGGAG 
ACCATTAGAT TAATATATTT CAAAAATAAG ATGGCAGAAG GGGATGAACT TGTGGTTCCT 
GAAACATCAG ATAATAGTCG GAAACAAATG GTTAGAAATA TCAACAACAA ACGGCGTTAC 
TCCTTCCGAG TCCCTGAAGA AGAGAGGATG CAACAGAGGA GGGAGATGCT ACGAGATCCA 
GAAATGAGAA ATAAATTAAT TTCTAACCCA ACTAATTTTA ACCACATAGC ACACATGGGT 
CCTGGAGATG GAATACAGAT CCTAAAAGAC CTGCCCATGC CAGGTTTCCC TTATTCACCC 
CCTCATCACC ACTCCGGCCT GATCTCCTCT CCGAGCAACT TTGAGCACAT CTATCACATG 
ACTGTCACTT CTGCTGAACA GTTTCTCTCT CCTGAGTCCA TAAGCCCTGG GTCCCCCCCG 
TCCCTGCGCT CTGCCCCTGG CACACCACAC TCTGTGACTC TGAGGAACCC ACGGCCTCAG 
GAAAGCCGGA CAGTATTCAG TGGCTCCGTC AGTATTCCCT CCATCACCAA GTCCCGCCCT 
GAGCCAGGCC GCTCCATGAG TGCCAGCAGC GGCCTGTCTG CACGGTCGTC AGCCCAGAAT 
GGCAGTGCAC TAAAGAGGGA ATTCTCTGGA GGAAGCTATA ACACAAAGCG ACAGCCCATG 
CCCTCTCCCT CGGAGGGCTC TTTGTCATCA GGAGGCATGG ACCAAGGAAG TGACGCCCCT 
GCGAGGGACT ATGATGGAGA GGACTCTGAT TCTCCAAGGC ATTCCACAGC TTCCAACAGC 
TCTAACCTGA GCAGCCCCCC AAGTCCCATT TCACCCCAAA AGACCAAGAG CCTCTCCCTG 
GAGAGCACAG ACCGCGGGAG CTGGGATCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_006496802.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496865.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X2, mRNA.

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CloneID	OMu28722
Clone ID Related Accession (Same CDS sequence)	XM_006496802.3 , XM_006496802.4
Accession Version	XM_006496802.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496802.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAAGATTCC CTTTCTTCTT CCTCATCCTC ACTGATTGAT 
TTTTTGGATG ACATTGCAGA CTGTGCTCCA CTTCCAGCTC ACACACCAAC CTTAAGGAAA 
AAGGGATGCC CGGCTTCAAC TGGCTTTCCA CCTAAGCGCA AGACTCATCA ATTTTTCGTG 
AAGTCTTTCA CTGCCCCTAC CAAGTGTCAC CAGTGCACCT CCTTGATGGT CGGTTTGATA 
AGACAGGGCT GTTCCTGTGA AGTCTGTGGG TTCTCATGTC ATATAACTTG TGTGAACAAA 
GCCCCGACGG TTTGCCCAGT TCCTCCTGAG CAAACTAAAG GTCCACTGGG CATAGACCCC 
CAGAAGGGAG TTGGGACGGC GTATGAGGGT CATGTCAGGA TCCCTAAGCC GGCGGGAGTG 
AAGAAAGGGT GGCAGAGAGC ACTGGCTGTG GTCTGCGACT TCAAACTGTT TCTTTATGAT 
ATCGCAGAGG GAAAAGCATC GCAGCCCACC TCCGTCATTA GTCAAGTGAT TGACATGAGA 
GATGAAGAAT TTTCTGTGAG TTCAGTCTTA GCTTCTGATG TTATTCACGC AAGTCGAAAA 
GATATCCCCT GTATATTCAG GGTCACAGCT TCCCAGCTCT CAGCACCTAG TAACAAATGT 
TCCATCCTGA TGCTAGCAGA CAGCGAGAAT GAGAGGAGTA AGTGGGTGGG AGTGCTGAGT 
GAACTCCACA AGATTCTGAA GAAAAACAAG TTCAGAGACC GCTCAGTGTA TGTCCCCAAG 
GAGGCGTACG ACAGCACGCT ACCCCTCATT AAGACAACGC AGGCCGCTGC AATCATAGAT 
CATGAACGGA TAGCTTTGGG AAATGAAGAA GGATTATTTG TTGTGCATGT CACCAAAGAT 
GAGATTGTTA GAGTTGGTGA TAATAAGAAA ATCCATCAGA TCGAACTCAT TCCAAGTGAC 
CAGCTTGTCG CTGTGATCTC AGGCCGAAAT CGCCACGTCC GACTTTTTCC TATGTCAGCC 
TTGGATGGGC GAGAGACGGA TTTTTATAAA CTGGCAGAGA CTAAAGGGTG TCAGACCATA 
GCTGCTGGGA AAGTGCGCCA CGGAGCTCTG TCCTGCCTGT GTGTGGCCAT GAAGAGACAG 
GTCCTCTGCT ATGAACTGTT CCAAAGCAAG ACCAGGCACA GGAAGTTTAA GGAGATTCAA 
GTCCCATGTA ATGTCCAGTG GATGGCCATC TTCAGTGAGC ATCTCTGTGT GGGATTCCAG 
TCAGGATTTC TCAGATACCC CCTGAATGGA GAAGGAGGTC CATGCAATAT GCTCCATTCA 
AATGACCATA CACTGTCATT CATTTCACAC CAACCAATGG ACGCTCTCTG TGCAGTTGAG 
ATCTCCAACA AAGAGTACTT GCTGTGTTTT AACAGCATTG GGATATACAC AGACTGCCAG 
GGCCGAAGAT CCAGACAGCA GGAACTGATG TGGCCAGCAA ATCCTTCCTC CTGTTGTTAC 
AATGCTCCAT ATCTCTCCGT GTATAGTGAA AACGCAGTTG ATATCTTTGA TGTAAACTCC 
ATGGAATGGA TACAGACTCT TCCTCTCAAA AAGGTCCGAC CTTTAAACAC TGAAGGATCA 
TTAAATCTCT TAGGATTGGA GACCATTAGA TTAATATATT TCAAAAATAA GATGGCAGAA 
GGGGATGAAC TTGTGGTTCC TGAAACATCA GATAATAGTC GGAAACAAAT GGTTAGAAAT 
ATCAACAACA AACGGCGTTA CTCCTTCCGA GTCCCTGAAG AAGAGAGGAT GCAACAGAGG 
AGGGAGATGC TACGAGATCC AGAAATGAGA AATAAATTAA TTTCTAACCC AACTAATTTT 
AACCACATAG CACACATGGG TCCTGGAGAT GGAATACAGA TCCTAAAAGA CCTGCCCATG 
CCAGGTTTCC CTTATTCACC CCCTCATCAC CACTCCGGCC TGATCTCCTC TCCGAGCAAC 
TTTGAGCACA TCTATCACAT GACTGTCACT TCTGCTGAAC AGTTTCTCTC TCCTGAGTCC 
ATAAGCCCTG GGTCCCCCCC GTCCCTGCGC TCTGCCCCTG GCACACCACA CTCTGTGACT 
CTGAGGAACC CACGGCCTCA GGAAAGCCGG ACAGTATTCA GTGGCTCCGT CAGTATTCCC 
TCCATCACCA AGTCCCGCCC TGAGCCAGGC CGCTCCATGA GTGCCAGCAG CGGCCTGTCT 
GCACGGTCGT CAGCCCAGAA TGGCAGTGCA CTAAAGAGGG AATTCTCTGG AGGAAGCTAT 
AACACAAAGC GACAGCCCAT GCCCTCTCCC TCGGAGGGCT CTTTGTCATC AGGAGGCATG 
GACCAAGGAA GTGACGCCCC TGCGAGGGAC TATGATGGAG AGGACTCTGA TTCTCCAAGG 
CATTCCACAG CTTCCAACAG CTCTAACCTG AGCAGCCCCC CAAGTCCCAT TTCACCCCAA 
AAGACCAAGA GCCTCTCCCT GGAGAGCACA GACCGCGGGA GCTGGGATCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496865.1
CDS	1032..6425
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSLSSSSSSLIDFLDDIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006496802.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496802.4

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAAGATTCC CTTTCTTCTT CCTCATCCTC ACTGATTGAT 
TTTTTGGATG ACATTGCAGA CTGTGCTCCA CTTCCAGCTC ACACACCAAC CTTAAGGAAA 
AAGGGATGCC CGGCTTCAAC TGGCTTTCCA CCTAAGCGCA AGACTCATCA ATTTTTCGTG 
AAGTCTTTCA CTGCCCCTAC CAAGTGTCAC CAGTGCACCT CCTTGATGGT CGGTTTGATA 
AGACAGGGCT GTTCCTGTGA AGTCTGTGGG TTCTCATGTC ATATAACTTG TGTGAACAAA 
GCCCCGACGG TTTGCCCAGT TCCTCCTGAG CAAACTAAAG GTCCACTGGG CATAGACCCC 
CAGAAGGGAG TTGGGACGGC GTATGAGGGT CATGTCAGGA TCCCTAAGCC GGCGGGAGTG 
AAGAAAGGGT GGCAGAGAGC ACTGGCTGTG GTCTGCGACT TCAAACTGTT TCTTTATGAT 
ATCGCAGAGG GAAAAGCATC GCAGCCCACC TCCGTCATTA GTCAAGTGAT TGACATGAGA 
GATGAAGAAT TTTCTGTGAG TTCAGTCTTA GCTTCTGATG TTATTCACGC AAGTCGAAAA 
GATATCCCCT GTATATTCAG GGTCACAGCT TCCCAGCTCT CAGCACCTAG TAACAAATGT 
TCCATCCTGA TGCTAGCAGA CAGCGAGAAT GAGAGGAGTA AGTGGGTGGG AGTGCTGAGT 
GAACTCCACA AGATTCTGAA GAAAAACAAG TTCAGAGACC GCTCAGTGTA TGTCCCCAAG 
GAGGCGTACG ACAGCACGCT ACCCCTCATT AAGACAACGC AGGCCGCTGC AATCATAGAT 
CATGAACGGA TAGCTTTGGG AAATGAAGAA GGATTATTTG TTGTGCATGT CACCAAAGAT 
GAGATTGTTA GAGTTGGTGA TAATAAGAAA ATCCATCAGA TCGAACTCAT TCCAAGTGAC 
CAGCTTGTCG CTGTGATCTC AGGCCGAAAT CGCCACGTCC GACTTTTTCC TATGTCAGCC 
TTGGATGGGC GAGAGACGGA TTTTTATAAA CTGGCAGAGA CTAAAGGGTG TCAGACCATA 
GCTGCTGGGA AAGTGCGCCA CGGAGCTCTG TCCTGCCTGT GTGTGGCCAT GAAGAGACAG 
GTCCTCTGCT ATGAACTGTT CCAAAGCAAG ACCAGGCACA GGAAGTTTAA GGAGATTCAA 
GTCCCATGTA ATGTCCAGTG GATGGCCATC TTCAGTGAGC ATCTCTGTGT GGGATTCCAG 
TCAGGATTTC TCAGATACCC CCTGAATGGA GAAGGAGGTC CATGCAATAT GCTCCATTCA 
AATGACCATA CACTGTCATT CATTTCACAC CAACCAATGG ACGCTCTCTG TGCAGTTGAG 
ATCTCCAACA AAGAGTACTT GCTGTGTTTT AACAGCATTG GGATATACAC AGACTGCCAG 
GGCCGAAGAT CCAGACAGCA GGAACTGATG TGGCCAGCAA ATCCTTCCTC CTGTTGTTAC 
AATGCTCCAT ATCTCTCCGT GTATAGTGAA AACGCAGTTG ATATCTTTGA TGTAAACTCC 
ATGGAATGGA TACAGACTCT TCCTCTCAAA AAGGTCCGAC CTTTAAACAC TGAAGGATCA 
TTAAATCTCT TAGGATTGGA GACCATTAGA TTAATATATT TCAAAAATAA GATGGCAGAA 
GGGGATGAAC TTGTGGTTCC TGAAACATCA GATAATAGTC GGAAACAAAT GGTTAGAAAT 
ATCAACAACA AACGGCGTTA CTCCTTCCGA GTCCCTGAAG AAGAGAGGAT GCAACAGAGG 
AGGGAGATGC TACGAGATCC AGAAATGAGA AATAAATTAA TTTCTAACCC AACTAATTTT 
AACCACATAG CACACATGGG TCCTGGAGAT GGAATACAGA TCCTAAAAGA CCTGCCCATG 
CCAGGTTTCC CTTATTCACC CCCTCATCAC CACTCCGGCC TGATCTCCTC TCCGAGCAAC 
TTTGAGCACA TCTATCACAT GACTGTCACT TCTGCTGAAC AGTTTCTCTC TCCTGAGTCC 
ATAAGCCCTG GGTCCCCCCC GTCCCTGCGC TCTGCCCCTG GCACACCACA CTCTGTGACT 
CTGAGGAACC CACGGCCTCA GGAAAGCCGG ACAGTATTCA GTGGCTCCGT CAGTATTCCC 
TCCATCACCA AGTCCCGCCC TGAGCCAGGC CGCTCCATGA GTGCCAGCAG CGGCCTGTCT 
GCACGGTCGT CAGCCCAGAA TGGCAGTGCA CTAAAGAGGG AATTCTCTGG AGGAAGCTAT 
AACACAAAGC GACAGCCCAT GCCCTCTCCC TCGGAGGGCT CTTTGTCATC AGGAGGCATG 
GACCAAGGAA GTGACGCCCC TGCGAGGGAC TATGATGGAG AGGACTCTGA TTCTCCAAGG 
CATTCCACAG CTTCCAACAG CTCTAACCTG AGCAGCCCCC CAAGTCCCAT TTCACCCCAA 
AAGACCAAGA GCCTCTCCCT GGAGAGCACA GACCGCGGGA GCTGGGATCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_006496803.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496866.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X3, mRNA.

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CloneID	OMu28723
Clone ID Related Accession (Same CDS sequence)	XM_006496803.4 , XM_006496803.3
Accession Version	XM_006496803.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496803.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAACGCTCT CCGTCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTATTGCAG ACTGTGCTCC ACTTCCAGCT CACACACCAA CCTTAAGGAA AAAGGGATGC 
CCGGCTTCAA CTGGCTTTCC ACCTAAGCGC AAGACTCATC AATTTTTCGT GAAGTCTTTC 
ACTGCCCCTA CCAAGTGTCA CCAGTGCACC TCCTTGATGG TCGGTTTGAT AAGACAGGGC 
TGTTCCTGTG AAGTCTGTGG GTTCTCATGT CATATAACTT GTGTGAACAA AGCCCCGACG 
GTTTGCCCAG TTCCTCCTGA GCAAACTAAA GGTCCACTGG GCATAGACCC CCAGAAGGGA 
GTTGGGACGG CGTATGAGGG TCATGTCAGG ATCCCTAAGC CGGCGGGAGT GAAGAAAGGG 
TGGCAGAGAG CACTGGCTGT GGTCTGCGAC TTCAAACTGT TTCTTTATGA TATCGCAGAG 
GGAAAAGCAT CGCAGCCCAC CTCCGTCATT AGTCAAGTGA TTGACATGAG AGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT AGCTTCTGAT GTTATTCACG CAAGTCGAAA AGATATCCCC 
TGTATATTCA GGGTCACAGC TTCCCAGCTC TCAGCACCTA GTAACAAATG TTCCATCCTG 
ATGCTAGCAG ACAGCGAGAA TGAGAGGAGT AAGTGGGTGG GAGTGCTGAG TGAACTCCAC 
AAGATTCTGA AGAAAAACAA GTTCAGAGAC CGCTCAGTGT ATGTCCCCAA GGAGGCGTAC 
GACAGCACGC TACCCCTCAT TAAGACAACG CAGGCCGCTG CAATCATAGA TCATGAACGG 
ATAGCTTTGG GAAATGAAGA AGGATTATTT GTTGTGCATG TCACCAAAGA TGAGATTGTT 
AGAGTTGGTG ATAATAAGAA AATCCATCAG ATCGAACTCA TTCCAAGTGA CCAGCTTGTC 
GCTGTGATCT CAGGCCGAAA TCGCCACGTC CGACTTTTTC CTATGTCAGC CTTGGATGGG 
CGAGAGACGG ATTTTTATAA ACTGGCAGAG ACTAAAGGGT GTCAGACCAT AGCTGCTGGG 
AAAGTGCGCC ACGGAGCTCT GTCCTGCCTG TGTGTGGCCA TGAAGAGACA GGTCCTCTGC 
TATGAACTGT TCCAAAGCAA GACCAGGCAC AGGAAGTTTA AGGAGATTCA AGTCCCATGT 
AATGTCCAGT GGATGGCCAT CTTCAGTGAG CATCTCTGTG TGGGATTCCA GTCAGGATTT 
CTCAGATACC CCCTGAATGG AGAAGGAGGT CCATGCAATA TGCTCCATTC AAATGACCAT 
ACACTGTCAT TCATTTCACA CCAACCAATG GACGCTCTCT GTGCAGTTGA GATCTCCAAC 
AAAGAGTACT TGCTGTGTTT TAACAGCATT GGGATATACA CAGACTGCCA GGGCCGAAGA 
TCCAGACAGC AGGAACTGAT GTGGCCAGCA AATCCTTCCT CCTGTTGTTA CAATGCTCCA 
TATCTCTCCG TGTATAGTGA AAACGCAGTT GATATCTTTG ATGTAAACTC CATGGAATGG 
ATACAGACTC TTCCTCTCAA AAAGGTCCGA CCTTTAAACA CTGAAGGATC ATTAAATCTC 
TTAGGATTGG AGACCATTAG ATTAATATAT TTCAAAAATA AGATGGCAGA AGGGGATGAA 
CTTGTGGTTC CTGAAACATC AGATAATAGT CGGAAACAAA TGGTTAGAAA TATCAACAAC 
AAACGGCGTT ACTCCTTCCG AGTCCCTGAA GAAGAGAGGA TGCAACAGAG GAGGGAGATG 
CTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAACC CAACTAATTT TAACCACATA 
GCACACATGG GTCCTGGAGA TGGAATACAG ATCCTAAAAG ACCTGCCCAT GCCAGGTTTC 
CCTTATTCAC CCCCTCATCA CCACTCCGGC CTGATCTCCT CTCCGAGCAA CTTTGAGCAC 
ATCTATCACA TGACTGTCAC TTCTGCTGAA CAGTTTCTCT CTCCTGAGTC CATAAGCCCT 
GGGTCCCCCC CGTCCCTGCG CTCTGCCCCT GGCACACCAC ACTCTGTGAC TCTGAGGAAC 
CCACGGCCTC AGGAAAGCCG GACAGTATTC AGTGGCTCCG TCAGTATTCC CTCCATCACC 
AAGTCCCGCC CTGAGCCAGG CCGCTCCATG AGTGCCAGCA GCGGCCTGTC TGCACGGTCG 
TCAGCCCAGA ATGGCAGTGC ACTAAAGAGG GAATTCTCTG GAGGAAGCTA TAACACAAAG 
CGACAGCCCA TGCCCTCTCC CTCGGAGGGC TCTTTGTCAT CAGGAGGCAT GGACCAAGGA 
AGTGACGCCC CTGCGAGGGA CTATGATGGA GAGGACTCTG ATTCTCCAAG GCATTCCACA 
GCTTCCAACA GCTCTAACCT GAGCAGCCCC CCAAGTCCCA TTTCACCCCA AAAGACCAAG 
AGCCTCTCCC TGGAGAGCAC AGACCGCGGG AGCTGGGATC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496866.1
CDS	1032..6416
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006496803.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496803.4

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAACGCTCT CCGTCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTATTGCAG ACTGTGCTCC ACTTCCAGCT CACACACCAA CCTTAAGGAA AAAGGGATGC 
CCGGCTTCAA CTGGCTTTCC ACCTAAGCGC AAGACTCATC AATTTTTCGT GAAGTCTTTC 
ACTGCCCCTA CCAAGTGTCA CCAGTGCACC TCCTTGATGG TCGGTTTGAT AAGACAGGGC 
TGTTCCTGTG AAGTCTGTGG GTTCTCATGT CATATAACTT GTGTGAACAA AGCCCCGACG 
GTTTGCCCAG TTCCTCCTGA GCAAACTAAA GGTCCACTGG GCATAGACCC CCAGAAGGGA 
GTTGGGACGG CGTATGAGGG TCATGTCAGG ATCCCTAAGC CGGCGGGAGT GAAGAAAGGG 
TGGCAGAGAG CACTGGCTGT GGTCTGCGAC TTCAAACTGT TTCTTTATGA TATCGCAGAG 
GGAAAAGCAT CGCAGCCCAC CTCCGTCATT AGTCAAGTGA TTGACATGAG AGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT AGCTTCTGAT GTTATTCACG CAAGTCGAAA AGATATCCCC 
TGTATATTCA GGGTCACAGC TTCCCAGCTC TCAGCACCTA GTAACAAATG TTCCATCCTG 
ATGCTAGCAG ACAGCGAGAA TGAGAGGAGT AAGTGGGTGG GAGTGCTGAG TGAACTCCAC 
AAGATTCTGA AGAAAAACAA GTTCAGAGAC CGCTCAGTGT ATGTCCCCAA GGAGGCGTAC 
GACAGCACGC TACCCCTCAT TAAGACAACG CAGGCCGCTG CAATCATAGA TCATGAACGG 
ATAGCTTTGG GAAATGAAGA AGGATTATTT GTTGTGCATG TCACCAAAGA TGAGATTGTT 
AGAGTTGGTG ATAATAAGAA AATCCATCAG ATCGAACTCA TTCCAAGTGA CCAGCTTGTC 
GCTGTGATCT CAGGCCGAAA TCGCCACGTC CGACTTTTTC CTATGTCAGC CTTGGATGGG 
CGAGAGACGG ATTTTTATAA ACTGGCAGAG ACTAAAGGGT GTCAGACCAT AGCTGCTGGG 
AAAGTGCGCC ACGGAGCTCT GTCCTGCCTG TGTGTGGCCA TGAAGAGACA GGTCCTCTGC 
TATGAACTGT TCCAAAGCAA GACCAGGCAC AGGAAGTTTA AGGAGATTCA AGTCCCATGT 
AATGTCCAGT GGATGGCCAT CTTCAGTGAG CATCTCTGTG TGGGATTCCA GTCAGGATTT 
CTCAGATACC CCCTGAATGG AGAAGGAGGT CCATGCAATA TGCTCCATTC AAATGACCAT 
ACACTGTCAT TCATTTCACA CCAACCAATG GACGCTCTCT GTGCAGTTGA GATCTCCAAC 
AAAGAGTACT TGCTGTGTTT TAACAGCATT GGGATATACA CAGACTGCCA GGGCCGAAGA 
TCCAGACAGC AGGAACTGAT GTGGCCAGCA AATCCTTCCT CCTGTTGTTA CAATGCTCCA 
TATCTCTCCG TGTATAGTGA AAACGCAGTT GATATCTTTG ATGTAAACTC CATGGAATGG 
ATACAGACTC TTCCTCTCAA AAAGGTCCGA CCTTTAAACA CTGAAGGATC ATTAAATCTC 
TTAGGATTGG AGACCATTAG ATTAATATAT TTCAAAAATA AGATGGCAGA AGGGGATGAA 
CTTGTGGTTC CTGAAACATC AGATAATAGT CGGAAACAAA TGGTTAGAAA TATCAACAAC 
AAACGGCGTT ACTCCTTCCG AGTCCCTGAA GAAGAGAGGA TGCAACAGAG GAGGGAGATG 
CTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAACC CAACTAATTT TAACCACATA 
GCACACATGG GTCCTGGAGA TGGAATACAG ATCCTAAAAG ACCTGCCCAT GCCAGGTTTC 
CCTTATTCAC CCCCTCATCA CCACTCCGGC CTGATCTCCT CTCCGAGCAA CTTTGAGCAC 
ATCTATCACA TGACTGTCAC TTCTGCTGAA CAGTTTCTCT CTCCTGAGTC CATAAGCCCT 
GGGTCCCCCC CGTCCCTGCG CTCTGCCCCT GGCACACCAC ACTCTGTGAC TCTGAGGAAC 
CCACGGCCTC AGGAAAGCCG GACAGTATTC AGTGGCTCCG TCAGTATTCC CTCCATCACC 
AAGTCCCGCC CTGAGCCAGG CCGCTCCATG AGTGCCAGCA GCGGCCTGTC TGCACGGTCG 
TCAGCCCAGA ATGGCAGTGC ACTAAAGAGG GAATTCTCTG GAGGAAGCTA TAACACAAAG 
CGACAGCCCA TGCCCTCTCC CTCGGAGGGC TCTTTGTCAT CAGGAGGCAT GGACCAAGGA 
AGTGACGCCC CTGCGAGGGA CTATGATGGA GAGGACTCTG ATTCTCCAAG GCATTCCACA 
GCTTCCAACA GCTCTAACCT GAGCAGCCCC CCAAGTCCCA TTTCACCCCA AAAGACCAAG 
AGCCTCTCCC TGGAGAGCAC AGACCGCGGG AGCTGGGATC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_006496804.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496867.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X4, mRNA.

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CloneID	OMu28724
Clone ID Related Accession (Same CDS sequence)	XM_006496804.3 , XM_006496804.4
Accession Version	XM_006496804.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496804.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGATT CCCTTTCTTC TTCCTCATCC TCACTGATTG ATTTTTTGGA TGACCGCTCT 
CCGTCCTGTA CTCCAGCTAG CAAAGGCAGA CGTATTGCAG ACTGTGCTCC ACTTCCAGCT 
CACACACCAA CCTTAAGGAA AAAGGGATGC CCGGCTTCAA CTGGCTTTCC ACCTAAGCGC 
AAGACTCATC AATTTTTCGT GAAGTCTTTC ACTGCCCCTA CCAAGTGTCA CCAGTGCACC 
TCCTTGATGG TCGGTTTGAT AAGACAGGGC TGTTCCTGTG AAGTCTGTGG GTTCTCATGT 
CATATAACTT GTGTGAACAA AGCCCCGACG GTTTGCCCAG TTCCTCCTGA GCAAACTAAA 
GGTCCACTGG GCATAGACCC CCAGAAGGGA GTTGGGACGG CGTATGAGGG TCATGTCAGG 
ATCCCTAAGC CGGCGGGAGT GAAGAAAGGG TGGCAGAGAG CACTGGCTGT GGTCTGCGAC 
TTCAAACTGT TTCTTTATGA TATCGCAGAG GGAAAAGCAT CGCAGCCCAC CTCCGTCATT 
AGTCAAGTGA TTGACATGAG AGATGAAGAA TTTTCTGTGA GTTCAGTCTT AGCTTCTGAT 
GTTATTCACG CAAGTCGAAA AGATATCCCC TGTATATTCA GGGTCACAGC TTCCCAGCTC 
TCAGCACCTA GTAACAAATG TTCCATCCTG ATGCTAGCAG ACAGCGAGAA TGAGAGGAGT 
AAGTGGGTGG GAGTGCTGAG TGAACTCCAC AAGATTCTGA AGAAAAACAA GTTCAGAGAC 
CGCTCAGTGT ATGTCCCCAA GGAGGCGTAC GACAGCACGC TACCCCTCAT TAAGACAACG 
CAGGCCGCTG CAATCATAGA TCATGAACGG ATAGCTTTGG GAAATGAAGA AGGATTATTT 
GTTGTGCATG TCACCAAAGA TGAGATTGTT AGAGTTGGTG ATAATAAGAA AATCCATCAG 
ATCGAACTCA TTCCAAGTGA CCAGCTTGTC GCTGTGATCT CAGGCCGAAA TCGCCACGTC 
CGACTTTTTC CTATGTCAGC CTTGGATGGG CGAGAGACGG ATTTTTATAA ACTGGCAGAG 
ACTAAAGGGT GTCAGACCAT AGCTGCTGGG AAAGTGCGCC ACGGAGCTCT GTCCTGCCTG 
TGTGTGGCCA TGAAGAGACA GGTCCTCTGC TATGAACTGT TCCAAAGCAA GACCAGGCAC 
AGGAAGTTTA AGGAGATTCA AGTCCCATGT AATGTCCAGT GGATGGCCAT CTTCAGTGAG 
CATCTCTGTG TGGGATTCCA GTCAGGATTT CTCAGATACC CCCTGAATGG AGAAGGAGGT 
CCATGCAATA TGCTCCATTC AAATGACCAT ACACTGTCAT TCATTTCACA CCAACCAATG 
GACGCTCTCT GTGCAGTTGA GATCTCCAAC AAAGAGTACT TGCTGTGTTT TAACAGCATT 
GGGATATACA CAGACTGCCA GGGCCGAAGA TCCAGACAGC AGGAACTGAT GTGGCCAGCA 
AATCCTTCCT CCTGTTGTTA CAATGCTCCA TATCTCTCCG TGTATAGTGA AAACGCAGTT 
GATATCTTTG ATGTAAACTC CATGGAATGG ATACAGACTC TTCCTCTCAA AAAGGTCCGA 
CCTTTAAACA CTGAAGGATC ATTAAATCTC TTAGGATTGG AGACCATTAG ATTAATATAT 
TTCAAAAATA AGATGGCAGA AGGGGATGAA CTTGTGGTTC CTGAAACATC AGATAATAGT 
CGGAAACAAA TGGTTAGAAA TATCAACAAC AAACGGCGTT ACTCCTTCCG AGTCCCTGAA 
GAAGAGAGGA TGCAACAGAG GAGGGAGATG CTACGAGATC CAGAAATGAG AAATAAATTA 
ATTTCTAACC CAACTAATTT TAACCACATA GCACACATGG GTCCTGGAGA TGGAATACAG 
ATCCTAAAAG ACCTGCCCAT GCCAGGTTTC CCTTATTCAC CCCCTCATCA CCACTCCGGC 
CTGATCTCCT CTCCGAGCAA CTTTGAGCAC ATCTATCACA TGACTGTCAC TTCTGCTGAA 
CAGTTTCTCT CTCCTGAGTC CATAAGCCCT GGGTCCCCCC CGTCCCTGCG CTCTGCCCCT 
GGCACACCAC ACTCTGTGAC TCTGAGGAAC CCACGGCCTC AGGAAAGCCG GACAGTATTC 
AGTGGCTCCG TCAGTATTCC CTCCATCACC AAGTCCCGCC CTGAGCCAGG CCGCTCCATG 
AGTGCCAGCA GCGGCCTGTC TGCACGGTCG TCAGCCCAGA ATGGCAGTGC ACTAAAGAGG 
GAATTCTCTG GAGGAAGCTA TAACACAAAG CGACAGCCCA TGCCCTCTCC CTCGGAGGGC 
TCTTTGTCAT CAGGAGGCAT GGACCAAGGA AGTGACGCCC CTGCGAGGGA CTATGATGGA 
GAGGACTCTG ATTCTCCAAG GCATTCCACA GCTTCCAACA GCTCTAACCT GAGCAGCCCC 
CCAAGTCCCA TTTCACCCCA AAAGACCAAG AGCCTCTCCC TGGAGAGCAC AGACCGCGGG 
AGCTGGGATC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496867.1
CDS	1032..6386
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKDSLSSSSSSLIDFLDDRSPSCTPASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006496804.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496804.4

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGATT CCCTTTCTTC TTCCTCATCC TCACTGATTG ATTTTTTGGA TGACCGCTCT 
CCGTCCTGTA CTCCAGCTAG CAAAGGCAGA CGTATTGCAG ACTGTGCTCC ACTTCCAGCT 
CACACACCAA CCTTAAGGAA AAAGGGATGC CCGGCTTCAA CTGGCTTTCC ACCTAAGCGC 
AAGACTCATC AATTTTTCGT GAAGTCTTTC ACTGCCCCTA CCAAGTGTCA CCAGTGCACC 
TCCTTGATGG TCGGTTTGAT AAGACAGGGC TGTTCCTGTG AAGTCTGTGG GTTCTCATGT 
CATATAACTT GTGTGAACAA AGCCCCGACG GTTTGCCCAG TTCCTCCTGA GCAAACTAAA 
GGTCCACTGG GCATAGACCC CCAGAAGGGA GTTGGGACGG CGTATGAGGG TCATGTCAGG 
ATCCCTAAGC CGGCGGGAGT GAAGAAAGGG TGGCAGAGAG CACTGGCTGT GGTCTGCGAC 
TTCAAACTGT TTCTTTATGA TATCGCAGAG GGAAAAGCAT CGCAGCCCAC CTCCGTCATT 
AGTCAAGTGA TTGACATGAG AGATGAAGAA TTTTCTGTGA GTTCAGTCTT AGCTTCTGAT 
GTTATTCACG CAAGTCGAAA AGATATCCCC TGTATATTCA GGGTCACAGC TTCCCAGCTC 
TCAGCACCTA GTAACAAATG TTCCATCCTG ATGCTAGCAG ACAGCGAGAA TGAGAGGAGT 
AAGTGGGTGG GAGTGCTGAG TGAACTCCAC AAGATTCTGA AGAAAAACAA GTTCAGAGAC 
CGCTCAGTGT ATGTCCCCAA GGAGGCGTAC GACAGCACGC TACCCCTCAT TAAGACAACG 
CAGGCCGCTG CAATCATAGA TCATGAACGG ATAGCTTTGG GAAATGAAGA AGGATTATTT 
GTTGTGCATG TCACCAAAGA TGAGATTGTT AGAGTTGGTG ATAATAAGAA AATCCATCAG 
ATCGAACTCA TTCCAAGTGA CCAGCTTGTC GCTGTGATCT CAGGCCGAAA TCGCCACGTC 
CGACTTTTTC CTATGTCAGC CTTGGATGGG CGAGAGACGG ATTTTTATAA ACTGGCAGAG 
ACTAAAGGGT GTCAGACCAT AGCTGCTGGG AAAGTGCGCC ACGGAGCTCT GTCCTGCCTG 
TGTGTGGCCA TGAAGAGACA GGTCCTCTGC TATGAACTGT TCCAAAGCAA GACCAGGCAC 
AGGAAGTTTA AGGAGATTCA AGTCCCATGT AATGTCCAGT GGATGGCCAT CTTCAGTGAG 
CATCTCTGTG TGGGATTCCA GTCAGGATTT CTCAGATACC CCCTGAATGG AGAAGGAGGT 
CCATGCAATA TGCTCCATTC AAATGACCAT ACACTGTCAT TCATTTCACA CCAACCAATG 
GACGCTCTCT GTGCAGTTGA GATCTCCAAC AAAGAGTACT TGCTGTGTTT TAACAGCATT 
GGGATATACA CAGACTGCCA GGGCCGAAGA TCCAGACAGC AGGAACTGAT GTGGCCAGCA 
AATCCTTCCT CCTGTTGTTA CAATGCTCCA TATCTCTCCG TGTATAGTGA AAACGCAGTT 
GATATCTTTG ATGTAAACTC CATGGAATGG ATACAGACTC TTCCTCTCAA AAAGGTCCGA 
CCTTTAAACA CTGAAGGATC ATTAAATCTC TTAGGATTGG AGACCATTAG ATTAATATAT 
TTCAAAAATA AGATGGCAGA AGGGGATGAA CTTGTGGTTC CTGAAACATC AGATAATAGT 
CGGAAACAAA TGGTTAGAAA TATCAACAAC AAACGGCGTT ACTCCTTCCG AGTCCCTGAA 
GAAGAGAGGA TGCAACAGAG GAGGGAGATG CTACGAGATC CAGAAATGAG AAATAAATTA 
ATTTCTAACC CAACTAATTT TAACCACATA GCACACATGG GTCCTGGAGA TGGAATACAG 
ATCCTAAAAG ACCTGCCCAT GCCAGGTTTC CCTTATTCAC CCCCTCATCA CCACTCCGGC 
CTGATCTCCT CTCCGAGCAA CTTTGAGCAC ATCTATCACA TGACTGTCAC TTCTGCTGAA 
CAGTTTCTCT CTCCTGAGTC CATAAGCCCT GGGTCCCCCC CGTCCCTGCG CTCTGCCCCT 
GGCACACCAC ACTCTGTGAC TCTGAGGAAC CCACGGCCTC AGGAAAGCCG GACAGTATTC 
AGTGGCTCCG TCAGTATTCC CTCCATCACC AAGTCCCGCC CTGAGCCAGG CCGCTCCATG 
AGTGCCAGCA GCGGCCTGTC TGCACGGTCG TCAGCCCAGA ATGGCAGTGC ACTAAAGAGG 
GAATTCTCTG GAGGAAGCTA TAACACAAAG CGACAGCCCA TGCCCTCTCC CTCGGAGGGC 
TCTTTGTCAT CAGGAGGCAT GGACCAAGGA AGTGACGCCC CTGCGAGGGA CTATGATGGA 
GAGGACTCTG ATTCTCCAAG GCATTCCACA GCTTCCAACA GCTCTAACCT GAGCAGCCCC 
CCAAGTCCCA TTTCACCCCA AAAGACCAAG AGCCTCTCCC TGGAGAGCAC AGACCGCGGG 
AGCTGGGATC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_006496806.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496869.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X5, mRNA.

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CloneID	OMu28726
Clone ID Related Accession (Same CDS sequence)	XM_006496806.4 , XM_006496806.3
Accession Version	XM_006496806.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496806.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGATT CCCTTTCTTC TTCCTCATCC TCACTGATTG ATTTTTTGGA TGACATTGCA 
GACTGTGCTC CACTTCCAGC TCACACACCA ACCTTAAGGA AAAAGGGATG CCCGGCTTCA 
ACTGGCTTTC CACCTAAGCG CAAGACTCAT CAATTTTTCG TGAAGTCTTT CACTGCCCCT 
ACCAAGTGTC ACCAGTGCAC CTCCTTGATG GTCGGTTTGA TAAGACAGGG CTGTTCCTGT 
GAAGTCTGTG GGTTCTCATG TCATATAACT TGTGTGAACA AAGCCCCGAC GGTTTGCCCA 
GTTCCTCCTG AGCAAACTAA AGGTCCACTG GGCATAGACC CCCAGAAGGG AGTTGGGACG 
GCGTATGAGG GTCATGTCAG GATCCCTAAG CCGGCGGGAG TGAAGAAAGG GTGGCAGAGA 
GCACTGGCTG TGGTCTGCGA CTTCAAACTG TTTCTTTATG ATATCGCAGA GGGAAAAGCA 
TCGCAGCCCA CCTCCGTCAT TAGTCAAGTG ATTGACATGA GAGATGAAGA ATTTTCTGTG 
AGTTCAGTCT TAGCTTCTGA TGTTATTCAC GCAAGTCGAA AAGATATCCC CTGTATATTC 
AGGGTCACAG CTTCCCAGCT CTCAGCACCT AGTAACAAAT GTTCCATCCT GATGCTAGCA 
GACAGCGAGA ATGAGAGGAG TAAGTGGGTG GGAGTGCTGA GTGAACTCCA CAAGATTCTG 
AAGAAAAACA AGTTCAGAGA CCGCTCAGTG TATGTCCCCA AGGAGGCGTA CGACAGCACG 
CTACCCCTCA TTAAGACAAC GCAGGCCGCT GCAATCATAG ATCATGAACG GATAGCTTTG 
GGAAATGAAG AAGGATTATT TGTTGTGCAT GTCACCAAAG ATGAGATTGT TAGAGTTGGT 
GATAATAAGA AAATCCATCA GATCGAACTC ATTCCAAGTG ACCAGCTTGT CGCTGTGATC 
TCAGGCCGAA ATCGCCACGT CCGACTTTTT CCTATGTCAG CCTTGGATGG GCGAGAGACG 
GATTTTTATA AACTGGCAGA GACTAAAGGG TGTCAGACCA TAGCTGCTGG GAAAGTGCGC 
CACGGAGCTC TGTCCTGCCT GTGTGTGGCC ATGAAGAGAC AGGTCCTCTG CTATGAACTG 
TTCCAAAGCA AGACCAGGCA CAGGAAGTTT AAGGAGATTC AAGTCCCATG TAATGTCCAG 
TGGATGGCCA TCTTCAGTGA GCATCTCTGT GTGGGATTCC AGTCAGGATT TCTCAGATAC 
CCCCTGAATG GAGAAGGAGG TCCATGCAAT ATGCTCCATT CAAATGACCA TACACTGTCA 
TTCATTTCAC ACCAACCAAT GGACGCTCTC TGTGCAGTTG AGATCTCCAA CAAAGAGTAC 
TTGCTGTGTT TTAACAGCAT TGGGATATAC ACAGACTGCC AGGGCCGAAG ATCCAGACAG 
CAGGAACTGA TGTGGCCAGC AAATCCTTCC TCCTGTTGTT ACAATGCTCC ATATCTCTCC 
GTGTATAGTG AAAACGCAGT TGATATCTTT GATGTAAACT CCATGGAATG GATACAGACT 
CTTCCTCTCA AAAAGGTCCG ACCTTTAAAC ACTGAAGGAT CATTAAATCT CTTAGGATTG 
GAGACCATTA GATTAATATA TTTCAAAAAT AAGATGGCAG AAGGGGATGA ACTTGTGGTT 
CCTGAAACAT CAGATAATAG TCGGAAACAA ATGGTTAGAA ATATCAACAA CAAACGGCGT 
TACTCCTTCC GAGTCCCTGA AGAAGAGAGG ATGCAACAGA GGAGGGAGAT GCTACGAGAT 
CCAGAAATGA GAAATAAATT AATTTCTAAC CCAACTAATT TTAACCACAT AGCACACATG 
GGTCCTGGAG ATGGAATACA GATCCTAAAA GACCTGCCCA TGCCAGGTTT CCCTTATTCA 
CCCCCTCATC ACCACTCCGG CCTGATCTCC TCTCCGAGCA ACTTTGAGCA CATCTATCAC 
ATGACTGTCA CTTCTGCTGA ACAGTTTCTC TCTCCTGAGT CCATAAGCCC TGGGTCCCCC 
CCGTCCCTGC GCTCTGCCCC TGGCACACCA CACTCTGTGA CTCTGAGGAA CCCACGGCCT 
CAGGAAAGCC GGACAGTATT CAGTGGCTCC GTCAGTATTC CCTCCATCAC CAAGTCCCGC 
CCTGAGCCAG GCCGCTCCAT GAGTGCCAGC AGCGGCCTGT CTGCACGGTC GTCAGCCCAG 
AATGGCAGTG CACTAAAGAG GGAATTCTCT GGAGGAAGCT ATAACACAAA GCGACAGCCC 
ATGCCCTCTC CCTCGGAGGG CTCTTTGTCA TCAGGAGGCA TGGACCAAGG AAGTGACGCC 
CCTGCGAGGG ACTATGATGG AGAGGACTCT GATTCTCCAA GGCATTCCAC AGCTTCCAAC 
AGCTCTAACC TGAGCAGCCC CCCAAGTCCC ATTTCACCCC AAAAGACCAA GAGCCTCTCC 
CTGGAGAGCA CAGACCGCGG GAGCTGGGAT CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496869.1
CDS	1032..6347
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKDSLSSSSSSLIDFLDDIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006496806.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496806.4

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGATT CCCTTTCTTC TTCCTCATCC TCACTGATTG ATTTTTTGGA TGACATTGCA 
GACTGTGCTC CACTTCCAGC TCACACACCA ACCTTAAGGA AAAAGGGATG CCCGGCTTCA 
ACTGGCTTTC CACCTAAGCG CAAGACTCAT CAATTTTTCG TGAAGTCTTT CACTGCCCCT 
ACCAAGTGTC ACCAGTGCAC CTCCTTGATG GTCGGTTTGA TAAGACAGGG CTGTTCCTGT 
GAAGTCTGTG GGTTCTCATG TCATATAACT TGTGTGAACA AAGCCCCGAC GGTTTGCCCA 
GTTCCTCCTG AGCAAACTAA AGGTCCACTG GGCATAGACC CCCAGAAGGG AGTTGGGACG 
GCGTATGAGG GTCATGTCAG GATCCCTAAG CCGGCGGGAG TGAAGAAAGG GTGGCAGAGA 
GCACTGGCTG TGGTCTGCGA CTTCAAACTG TTTCTTTATG ATATCGCAGA GGGAAAAGCA 
TCGCAGCCCA CCTCCGTCAT TAGTCAAGTG ATTGACATGA GAGATGAAGA ATTTTCTGTG 
AGTTCAGTCT TAGCTTCTGA TGTTATTCAC GCAAGTCGAA AAGATATCCC CTGTATATTC 
AGGGTCACAG CTTCCCAGCT CTCAGCACCT AGTAACAAAT GTTCCATCCT GATGCTAGCA 
GACAGCGAGA ATGAGAGGAG TAAGTGGGTG GGAGTGCTGA GTGAACTCCA CAAGATTCTG 
AAGAAAAACA AGTTCAGAGA CCGCTCAGTG TATGTCCCCA AGGAGGCGTA CGACAGCACG 
CTACCCCTCA TTAAGACAAC GCAGGCCGCT GCAATCATAG ATCATGAACG GATAGCTTTG 
GGAAATGAAG AAGGATTATT TGTTGTGCAT GTCACCAAAG ATGAGATTGT TAGAGTTGGT 
GATAATAAGA AAATCCATCA GATCGAACTC ATTCCAAGTG ACCAGCTTGT CGCTGTGATC 
TCAGGCCGAA ATCGCCACGT CCGACTTTTT CCTATGTCAG CCTTGGATGG GCGAGAGACG 
GATTTTTATA AACTGGCAGA GACTAAAGGG TGTCAGACCA TAGCTGCTGG GAAAGTGCGC 
CACGGAGCTC TGTCCTGCCT GTGTGTGGCC ATGAAGAGAC AGGTCCTCTG CTATGAACTG 
TTCCAAAGCA AGACCAGGCA CAGGAAGTTT AAGGAGATTC AAGTCCCATG TAATGTCCAG 
TGGATGGCCA TCTTCAGTGA GCATCTCTGT GTGGGATTCC AGTCAGGATT TCTCAGATAC 
CCCCTGAATG GAGAAGGAGG TCCATGCAAT ATGCTCCATT CAAATGACCA TACACTGTCA 
TTCATTTCAC ACCAACCAAT GGACGCTCTC TGTGCAGTTG AGATCTCCAA CAAAGAGTAC 
TTGCTGTGTT TTAACAGCAT TGGGATATAC ACAGACTGCC AGGGCCGAAG ATCCAGACAG 
CAGGAACTGA TGTGGCCAGC AAATCCTTCC TCCTGTTGTT ACAATGCTCC ATATCTCTCC 
GTGTATAGTG AAAACGCAGT TGATATCTTT GATGTAAACT CCATGGAATG GATACAGACT 
CTTCCTCTCA AAAAGGTCCG ACCTTTAAAC ACTGAAGGAT CATTAAATCT CTTAGGATTG 
GAGACCATTA GATTAATATA TTTCAAAAAT AAGATGGCAG AAGGGGATGA ACTTGTGGTT 
CCTGAAACAT CAGATAATAG TCGGAAACAA ATGGTTAGAA ATATCAACAA CAAACGGCGT 
TACTCCTTCC GAGTCCCTGA AGAAGAGAGG ATGCAACAGA GGAGGGAGAT GCTACGAGAT 
CCAGAAATGA GAAATAAATT AATTTCTAAC CCAACTAATT TTAACCACAT AGCACACATG 
GGTCCTGGAG ATGGAATACA GATCCTAAAA GACCTGCCCA TGCCAGGTTT CCCTTATTCA 
CCCCCTCATC ACCACTCCGG CCTGATCTCC TCTCCGAGCA ACTTTGAGCA CATCTATCAC 
ATGACTGTCA CTTCTGCTGA ACAGTTTCTC TCTCCTGAGT CCATAAGCCC TGGGTCCCCC 
CCGTCCCTGC GCTCTGCCCC TGGCACACCA CACTCTGTGA CTCTGAGGAA CCCACGGCCT 
CAGGAAAGCC GGACAGTATT CAGTGGCTCC GTCAGTATTC CCTCCATCAC CAAGTCCCGC 
CCTGAGCCAG GCCGCTCCAT GAGTGCCAGC AGCGGCCTGT CTGCACGGTC GTCAGCCCAG 
AATGGCAGTG CACTAAAGAG GGAATTCTCT GGAGGAAGCT ATAACACAAA GCGACAGCCC 
ATGCCCTCTC CCTCGGAGGG CTCTTTGTCA TCAGGAGGCA TGGACCAAGG AAGTGACGCC 
CCTGCGAGGG ACTATGATGG AGAGGACTCT GATTCTCCAA GGCATTCCAC AGCTTCCAAC 
AGCTCTAACC TGAGCAGCCC CCCAAGTCCC ATTTCACCCC AAAAGACCAA GAGCCTCTCC 
CTGGAGAGCA CAGACCGCGG GAGCTGGGAT CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_006496807.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496870.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X6, mRNA.

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CloneID	OMu28727
Clone ID Related Accession (Same CDS sequence)	XM_006496807.3 , XM_006496807.4
Accession Version	XM_006496807.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496807.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGCTA GCAAAGGCAG ACGTATTGCA GACTGTGCTC CACTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG CCCGGCTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAT 
CAATTTTTCG TGAAGTCTTT CACTGCCCCT ACCAAGTGTC ACCAGTGCAC CTCCTTGATG 
GTCGGTTTGA TAAGACAGGG CTGTTCCTGT GAAGTCTGTG GGTTCTCATG TCATATAACT 
TGTGTGAACA AAGCCCCGAC GGTTTGCCCA GTTCCTCCTG AGCAAACTAA AGGTCCACTG 
GGCATAGACC CCCAGAAGGG AGTTGGGACG GCGTATGAGG GTCATGTCAG GATCCCTAAG 
CCGGCGGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTG TGGTCTGCGA CTTCAAACTG 
TTTCTTTATG ATATCGCAGA GGGAAAAGCA TCGCAGCCCA CCTCCGTCAT TAGTCAAGTG 
ATTGACATGA GAGATGAAGA ATTTTCTGTG AGTTCAGTCT TAGCTTCTGA TGTTATTCAC 
GCAAGTCGAA AAGATATCCC CTGTATATTC AGGGTCACAG CTTCCCAGCT CTCAGCACCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCA GACAGCGAGA ATGAGAGGAG TAAGTGGGTG 
GGAGTGCTGA GTGAACTCCA CAAGATTCTG AAGAAAAACA AGTTCAGAGA CCGCTCAGTG 
TATGTCCCCA AGGAGGCGTA CGACAGCACG CTACCCCTCA TTAAGACAAC GCAGGCCGCT 
GCAATCATAG ATCATGAACG GATAGCTTTG GGAAATGAAG AAGGATTATT TGTTGTGCAT 
GTCACCAAAG ATGAGATTGT TAGAGTTGGT GATAATAAGA AAATCCATCA GATCGAACTC 
ATTCCAAGTG ACCAGCTTGT CGCTGTGATC TCAGGCCGAA ATCGCCACGT CCGACTTTTT 
CCTATGTCAG CCTTGGATGG GCGAGAGACG GATTTTTATA AACTGGCAGA GACTAAAGGG 
TGTCAGACCA TAGCTGCTGG GAAAGTGCGC CACGGAGCTC TGTCCTGCCT GTGTGTGGCC 
ATGAAGAGAC AGGTCCTCTG CTATGAACTG TTCCAAAGCA AGACCAGGCA CAGGAAGTTT 
AAGGAGATTC AAGTCCCATG TAATGTCCAG TGGATGGCCA TCTTCAGTGA GCATCTCTGT 
GTGGGATTCC AGTCAGGATT TCTCAGATAC CCCCTGAATG GAGAAGGAGG TCCATGCAAT 
ATGCTCCATT CAAATGACCA TACACTGTCA TTCATTTCAC ACCAACCAAT GGACGCTCTC 
TGTGCAGTTG AGATCTCCAA CAAAGAGTAC TTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACAGACTGCC AGGGCCGAAG ATCCAGACAG CAGGAACTGA TGTGGCCAGC AAATCCTTCC 
TCCTGTTGTT ACAATGCTCC ATATCTCTCC GTGTATAGTG AAAACGCAGT TGATATCTTT 
GATGTAAACT CCATGGAATG GATACAGACT CTTCCTCTCA AAAAGGTCCG ACCTTTAAAC 
ACTGAAGGAT CATTAAATCT CTTAGGATTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTTGTGGTT CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ATATCAACAA CAAACGGCGT TACTCCTTCC GAGTCCCTGA AGAAGAGAGG 
ATGCAACAGA GGAGGGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGCCAGGTTT CCCTTATTCA CCCCCTCATC ACCACTCCGG CCTGATCTCC 
TCTCCGAGCA ACTTTGAGCA CATCTATCAC ATGACTGTCA CTTCTGCTGA ACAGTTTCTC 
TCTCCTGAGT CCATAAGCCC TGGGTCCCCC CCGTCCCTGC GCTCTGCCCC TGGCACACCA 
CACTCTGTGA CTCTGAGGAA CCCACGGCCT CAGGAAAGCC GGACAGTATT CAGTGGCTCC 
GTCAGTATTC CCTCCATCAC CAAGTCCCGC CCTGAGCCAG GCCGCTCCAT GAGTGCCAGC 
AGCGGCCTGT CTGCACGGTC GTCAGCCCAG AATGGCAGTG CACTAAAGAG GGAATTCTCT 
GGAGGAAGCT ATAACACAAA GCGACAGCCC ATGCCCTCTC CCTCGGAGGG CTCTTTGTCA 
TCAGGAGGCA TGGACCAAGG AAGTGACGCC CCTGCGAGGG ACTATGATGG AGAGGACTCT 
GATTCTCCAA GGCATTCCAC AGCTTCCAAC AGCTCTAACC TGAGCAGCCC CCCAAGTCCC 
ATTTCACCCC AAAAGACCAA GAGCCTCTCC CTGGAGAGCA CAGACCGCGG GAGCTGGGAT 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496870.1
CDS	1032..6317
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006496807.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496807.4

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGCTA GCAAAGGCAG ACGTATTGCA GACTGTGCTC CACTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG CCCGGCTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAT 
CAATTTTTCG TGAAGTCTTT CACTGCCCCT ACCAAGTGTC ACCAGTGCAC CTCCTTGATG 
GTCGGTTTGA TAAGACAGGG CTGTTCCTGT GAAGTCTGTG GGTTCTCATG TCATATAACT 
TGTGTGAACA AAGCCCCGAC GGTTTGCCCA GTTCCTCCTG AGCAAACTAA AGGTCCACTG 
GGCATAGACC CCCAGAAGGG AGTTGGGACG GCGTATGAGG GTCATGTCAG GATCCCTAAG 
CCGGCGGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTG TGGTCTGCGA CTTCAAACTG 
TTTCTTTATG ATATCGCAGA GGGAAAAGCA TCGCAGCCCA CCTCCGTCAT TAGTCAAGTG 
ATTGACATGA GAGATGAAGA ATTTTCTGTG AGTTCAGTCT TAGCTTCTGA TGTTATTCAC 
GCAAGTCGAA AAGATATCCC CTGTATATTC AGGGTCACAG CTTCCCAGCT CTCAGCACCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCA GACAGCGAGA ATGAGAGGAG TAAGTGGGTG 
GGAGTGCTGA GTGAACTCCA CAAGATTCTG AAGAAAAACA AGTTCAGAGA CCGCTCAGTG 
TATGTCCCCA AGGAGGCGTA CGACAGCACG CTACCCCTCA TTAAGACAAC GCAGGCCGCT 
GCAATCATAG ATCATGAACG GATAGCTTTG GGAAATGAAG AAGGATTATT TGTTGTGCAT 
GTCACCAAAG ATGAGATTGT TAGAGTTGGT GATAATAAGA AAATCCATCA GATCGAACTC 
ATTCCAAGTG ACCAGCTTGT CGCTGTGATC TCAGGCCGAA ATCGCCACGT CCGACTTTTT 
CCTATGTCAG CCTTGGATGG GCGAGAGACG GATTTTTATA AACTGGCAGA GACTAAAGGG 
TGTCAGACCA TAGCTGCTGG GAAAGTGCGC CACGGAGCTC TGTCCTGCCT GTGTGTGGCC 
ATGAAGAGAC AGGTCCTCTG CTATGAACTG TTCCAAAGCA AGACCAGGCA CAGGAAGTTT 
AAGGAGATTC AAGTCCCATG TAATGTCCAG TGGATGGCCA TCTTCAGTGA GCATCTCTGT 
GTGGGATTCC AGTCAGGATT TCTCAGATAC CCCCTGAATG GAGAAGGAGG TCCATGCAAT 
ATGCTCCATT CAAATGACCA TACACTGTCA TTCATTTCAC ACCAACCAAT GGACGCTCTC 
TGTGCAGTTG AGATCTCCAA CAAAGAGTAC TTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACAGACTGCC AGGGCCGAAG ATCCAGACAG CAGGAACTGA TGTGGCCAGC AAATCCTTCC 
TCCTGTTGTT ACAATGCTCC ATATCTCTCC GTGTATAGTG AAAACGCAGT TGATATCTTT 
GATGTAAACT CCATGGAATG GATACAGACT CTTCCTCTCA AAAAGGTCCG ACCTTTAAAC 
ACTGAAGGAT CATTAAATCT CTTAGGATTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTTGTGGTT CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ATATCAACAA CAAACGGCGT TACTCCTTCC GAGTCCCTGA AGAAGAGAGG 
ATGCAACAGA GGAGGGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGCCAGGTTT CCCTTATTCA CCCCCTCATC ACCACTCCGG CCTGATCTCC 
TCTCCGAGCA ACTTTGAGCA CATCTATCAC ATGACTGTCA CTTCTGCTGA ACAGTTTCTC 
TCTCCTGAGT CCATAAGCCC TGGGTCCCCC CCGTCCCTGC GCTCTGCCCC TGGCACACCA 
CACTCTGTGA CTCTGAGGAA CCCACGGCCT CAGGAAAGCC GGACAGTATT CAGTGGCTCC 
GTCAGTATTC CCTCCATCAC CAAGTCCCGC CCTGAGCCAG GCCGCTCCAT GAGTGCCAGC 
AGCGGCCTGT CTGCACGGTC GTCAGCCCAG AATGGCAGTG CACTAAAGAG GGAATTCTCT 
GGAGGAAGCT ATAACACAAA GCGACAGCCC ATGCCCTCTC CCTCGGAGGG CTCTTTGTCA 
TCAGGAGGCA TGGACCAAGG AAGTGACGCC CCTGCGAGGG ACTATGATGG AGAGGACTCT 
GATTCTCCAA GGCATTCCAC AGCTTCCAAC AGCTCTAACC TGAGCAGCCC CCCAAGTCCC 
ATTTCACCCC AAAAGACCAA GAGCCTCTCC CTGGAGAGCA CAGACCGCGG GAGCTGGGAT 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_006496809.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496872.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X8, mRNA.

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CloneID	OMu28729
Clone ID Related Accession (Same CDS sequence)	XM_006496809.3 , XM_006496809.4
Accession Version	XM_006496809.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496809.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGCTG GAAGTTCACA CCGAAGCTCT AATTGCTGAA 
GCATCAAAGG ACAAGAAGCT CCGAGAGCAG AGCGAACATT ATTCTAAGCA ACTGGAAAAC 
GAGTTGGAGG GCCTGAAGCA AAAACAAATT AGTTACTCAC CAGGAATATG CAGCATAGAA 
CATCAGCAAG AGATAACTAA ACTGAAAACT GACTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAAATTT CTAAAAGAGA AGGAATTCAC GCAAGTGAGA TTAAAAACCT GAAGAAGGAG 
CTGCATGACT CAGAAGGCCA GCAGCTTGCT CTCAACAAAG AGATTCTGGT TTTAAAAGAC 
AAATTGGAAA AAACCAGGAG AGAAAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAACAACAAT ATGAACGGGA AAAAGTATTA TTAACTGAAG AGAATAAAAA GTTAACGAGT 
GAACTCGATA AGCTGACTTC ACTGTATGAG AGCTTAAGTT TGCGCAACCA GCACTTAGAA 
GAAGAAGTCA AAGATCTAGC AGATAAGAAG GAATCAGTTG CCCACTGGGA AGCCCAAATC 
ACAGAGATAA TTCAGTGGGT GAGCGATGAG AAGGATGCAC GAGGCTATCT TCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA GTTGGAAGCA TTAAGAAATT CCAGCTTGGG GACACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCAAAACTGG ACATGTCGGC TAGACTAGAG 
TTGCAGTCTG CTCTGGATGC AGAAATAAGA GCTAAGCAGG CCATCCAGGA AGAGCTGAAT 
AAAGTCAAAG CATCTAACAT CTTAACAGAA TGTAAACTGA AAGATTCAGA GAAGAAGAAC 
TTGGAACTGT TGTCCGAAAT CGAGCAGCTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ACCAATTTGA AGATTCCCTT TCTTCTTCCT CATCCTCACT GATTGATTTT 
TTGGATGACC GCTCTCCGTC CTGTACTCCA GCTAGCAAAG GCAGACGTAT TGCAGACTGT 
GCTCCACTTC CAGCTCACAC ACCAACCTTA AGGAAAAAGG GATGCCCGGC TTCAACTGGC 
TTTCCACCTA AGCGCAAGAC TCATCAATTT TTCGTGAAGT CTTTCACTGC CCCTACCAAG 
TGTCACCAGT GCACCTCCTT GATGGTCGGT TTGATAAGAC AGGGCTGTTC CTGTGAAGTC 
TGTGGGTTCT CATGTCATAT AACTTGTGTG AACAAAGCCC CGACGGTTTG CCCAGTTCCT 
CCTGAGCAAA CTAAAGGTCC ACTGGGCATA GACCCCCAGA AGGGAGTTGG GACGGCGTAT 
GAGGGTCATG TCAGGATCCC TAAGCCGGCG GGAGTGAAGA AAGGGTGGCA GAGAGCACTG 
GCTGTGGTCT GCGACTTCAA ACTGTTTCTT TATGATATCG CAGAGGGAAA AGCATCGCAG 
CCCACCTCCG TCATTAGTCA AGTGATTGAC ATGAGAGATG AAGAATTTTC TGTGAGTTCA 
GTCTTAGCTT CTGATGTTAT TCACGCAAGT CGAAAAGATA TCCCCTGTAT ATTCAGGGTC 
ACAGCTTCCC AGCTCTCAGC ACCTAGTAAC AAATGTTCCA TCCTGATGCT AGCAGACAGC 
GAGAATGAGA GGAGTAAGTG GGTGGGAGTG CTGAGTGAAC TCCACAAGAT TCTGAAGAAA 
AACAAGTTCA GAGACCGCTC AGTGTATGTC CCCAAGGAGG CGTACGACAG CACGCTACCC 
CTCATTAAGA CAACGCAGGC CGCTGCAATC ATAGATCATG AACGGATAGC TTTGGGAAAT 
GAAGAAGGAT TATTTGTTGT GCATGTCACC AAAGATGAGA TTGTTAGAGT TGGTGATAAT 
AAGAAAATCC ATCAGATCGA ACTCATTCCA AGTGACCAGC TTGTCGCTGT GATCTCAGGC 
CGAAATCGCC ACGTCCGACT TTTTCCTATG TCAGCCTTGG ATGGGCGAGA GACGGATTTT 
TATAAACTGG CAGAGACTAA AGGGTGTCAG ACCATAGCTG CTGGGAAAGT GCGCCACGGA 
GCTCTGTCCT GCCTGTGTGT GGCCATGAAG AGACAGGTCC TCTGCTATGA ACTGTTCCAA 
AGCAAGACCA GGCACAGGAA GTTTAAGGAG ATTCAAGTCC CATGTAATGT CCAGTGGATG 
GCCATCTTCA GTGAGCATCT CTGTGTGGGA TTCCAGTCAG GATTTCTCAG ATACCCCCTG 
AATGGAGAAG GAGGTCCATG CAATATGCTC CATTCAAATG ACCATACACT GTCATTCATT 
TCACACCAAC CAATGGACGC TCTCTGTGCA GTTGAGATCT CCAACAAAGA GTACTTGCTG 
TGTTTTAACA GCATTGGGAT ATACACAGAC TGCCAGGGCC GAAGATCCAG ACAGCAGGAA 
CTGATGTGGC CAGCAAATCC TTCCTCCTGT TGTTACAATG CTCCATATCT CTCCGTGTAT 
AGTGAAAACG CAGTTGATAT CTTTGATGTA AACTCCATGG AATGGATACA GACTCTTCCT 
CTCAAAAAGG TCCGACCTTT AAACACTGAA GGATCATTAA ATCTCTTAGG ATTGGAGACC 
ATTAGATTAA TATATTTCAA AAATAAGATG GCAGAAGGGG ATGAACTTGT GGTTCCTGAA 
ACATCAGATA ATAGTCGGAA ACAAATGGTT AGAAATATCA ACAACAAACG GCGTTACTCC 
TTCCGAGTCC CTGAAGAAGA GAGGATGCAA CAGAGGAGGG AGATGCTACG AGATCCAGAA 
ATGAGAAATA AATTAATTTC TAACCCAACT AATTTTAACC ACATAGCACA CATGGGTCCT 
GGAGATGGAA TACAGATCCT AAAAGACCTG CCCATGCCAG GTTTCCCTTA TTCACCCCCT 
CATCACCACT CCGGCCTGAT CTCCTCTCCG AGCAACTTTG AGCACATCTA TCACATGACT 
GTCACTTCTG CTGAACAGTT TCTCTCTCCT GAGTCCATAA GCCCTGGGTC CCCCCCGTCC 
CTGCGCTCTG CCCCTGGCAC ACCACACTCT GTGACTCTGA GGAACCCACG GCCTCAGGAA 
AGCCGGACAG TATTCAGTGG CTCCGTCAGT ATTCCCTCCA TCACCAAGTC CCGCCCTGAG 
CCAGGCCGCT CCATGAGTGC CAGCAGCGGC CTGTCTGCAC GGTCGTCAGC CCAGAATGGC 
AGTGCACTAA AGAGGGAATT CTCTGGAGGA AGCTATAACA CAAAGCGACA GCCCATGCCC 
TCTCCCTCGG AGGGCTCTTT GTCATCAGGA GGCATGGACC AAGGAAGTGA CGCCCCTGCG 
AGGGACTATG ATGGAGAGGA CTCTGATTCT CCAAGGCATT CCACAGCTTC CAACAGCTCT 
AACCTGAGCA GCCCCCCAAG TCCCATTTCA CCCCAAAAGA CCAAGAGCCT CTCCCTGGAG 
AGCACAGACC GCGGGAGCTG GGATCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496872.1
CDS	1032..6221
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKLEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSLSSSSSSLIDFLDDRSPSCTPASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006496809.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496809.4

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGCTG GAAGTTCACA CCGAAGCTCT AATTGCTGAA 
GCATCAAAGG ACAAGAAGCT CCGAGAGCAG AGCGAACATT ATTCTAAGCA ACTGGAAAAC 
GAGTTGGAGG GCCTGAAGCA AAAACAAATT AGTTACTCAC CAGGAATATG CAGCATAGAA 
CATCAGCAAG AGATAACTAA ACTGAAAACT GACTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAAATTT CTAAAAGAGA AGGAATTCAC GCAAGTGAGA TTAAAAACCT GAAGAAGGAG 
CTGCATGACT CAGAAGGCCA GCAGCTTGCT CTCAACAAAG AGATTCTGGT TTTAAAAGAC 
AAATTGGAAA AAACCAGGAG AGAAAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAACAACAAT ATGAACGGGA AAAAGTATTA TTAACTGAAG AGAATAAAAA GTTAACGAGT 
GAACTCGATA AGCTGACTTC ACTGTATGAG AGCTTAAGTT TGCGCAACCA GCACTTAGAA 
GAAGAAGTCA AAGATCTAGC AGATAAGAAG GAATCAGTTG CCCACTGGGA AGCCCAAATC 
ACAGAGATAA TTCAGTGGGT GAGCGATGAG AAGGATGCAC GAGGCTATCT TCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA GTTGGAAGCA TTAAGAAATT CCAGCTTGGG GACACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCAAAACTGG ACATGTCGGC TAGACTAGAG 
TTGCAGTCTG CTCTGGATGC AGAAATAAGA GCTAAGCAGG CCATCCAGGA AGAGCTGAAT 
AAAGTCAAAG CATCTAACAT CTTAACAGAA TGTAAACTGA AAGATTCAGA GAAGAAGAAC 
TTGGAACTGT TGTCCGAAAT CGAGCAGCTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ACCAATTTGA AGATTCCCTT TCTTCTTCCT CATCCTCACT GATTGATTTT 
TTGGATGACC GCTCTCCGTC CTGTACTCCA GCTAGCAAAG GCAGACGTAT TGCAGACTGT 
GCTCCACTTC CAGCTCACAC ACCAACCTTA AGGAAAAAGG GATGCCCGGC TTCAACTGGC 
TTTCCACCTA AGCGCAAGAC TCATCAATTT TTCGTGAAGT CTTTCACTGC CCCTACCAAG 
TGTCACCAGT GCACCTCCTT GATGGTCGGT TTGATAAGAC AGGGCTGTTC CTGTGAAGTC 
TGTGGGTTCT CATGTCATAT AACTTGTGTG AACAAAGCCC CGACGGTTTG CCCAGTTCCT 
CCTGAGCAAA CTAAAGGTCC ACTGGGCATA GACCCCCAGA AGGGAGTTGG GACGGCGTAT 
GAGGGTCATG TCAGGATCCC TAAGCCGGCG GGAGTGAAGA AAGGGTGGCA GAGAGCACTG 
GCTGTGGTCT GCGACTTCAA ACTGTTTCTT TATGATATCG CAGAGGGAAA AGCATCGCAG 
CCCACCTCCG TCATTAGTCA AGTGATTGAC ATGAGAGATG AAGAATTTTC TGTGAGTTCA 
GTCTTAGCTT CTGATGTTAT TCACGCAAGT CGAAAAGATA TCCCCTGTAT ATTCAGGGTC 
ACAGCTTCCC AGCTCTCAGC ACCTAGTAAC AAATGTTCCA TCCTGATGCT AGCAGACAGC 
GAGAATGAGA GGAGTAAGTG GGTGGGAGTG CTGAGTGAAC TCCACAAGAT TCTGAAGAAA 
AACAAGTTCA GAGACCGCTC AGTGTATGTC CCCAAGGAGG CGTACGACAG CACGCTACCC 
CTCATTAAGA CAACGCAGGC CGCTGCAATC ATAGATCATG AACGGATAGC TTTGGGAAAT 
GAAGAAGGAT TATTTGTTGT GCATGTCACC AAAGATGAGA TTGTTAGAGT TGGTGATAAT 
AAGAAAATCC ATCAGATCGA ACTCATTCCA AGTGACCAGC TTGTCGCTGT GATCTCAGGC 
CGAAATCGCC ACGTCCGACT TTTTCCTATG TCAGCCTTGG ATGGGCGAGA GACGGATTTT 
TATAAACTGG CAGAGACTAA AGGGTGTCAG ACCATAGCTG CTGGGAAAGT GCGCCACGGA 
GCTCTGTCCT GCCTGTGTGT GGCCATGAAG AGACAGGTCC TCTGCTATGA ACTGTTCCAA 
AGCAAGACCA GGCACAGGAA GTTTAAGGAG ATTCAAGTCC CATGTAATGT CCAGTGGATG 
GCCATCTTCA GTGAGCATCT CTGTGTGGGA TTCCAGTCAG GATTTCTCAG ATACCCCCTG 
AATGGAGAAG GAGGTCCATG CAATATGCTC CATTCAAATG ACCATACACT GTCATTCATT 
TCACACCAAC CAATGGACGC TCTCTGTGCA GTTGAGATCT CCAACAAAGA GTACTTGCTG 
TGTTTTAACA GCATTGGGAT ATACACAGAC TGCCAGGGCC GAAGATCCAG ACAGCAGGAA 
CTGATGTGGC CAGCAAATCC TTCCTCCTGT TGTTACAATG CTCCATATCT CTCCGTGTAT 
AGTGAAAACG CAGTTGATAT CTTTGATGTA AACTCCATGG AATGGATACA GACTCTTCCT 
CTCAAAAAGG TCCGACCTTT AAACACTGAA GGATCATTAA ATCTCTTAGG ATTGGAGACC 
ATTAGATTAA TATATTTCAA AAATAAGATG GCAGAAGGGG ATGAACTTGT GGTTCCTGAA 
ACATCAGATA ATAGTCGGAA ACAAATGGTT AGAAATATCA ACAACAAACG GCGTTACTCC 
TTCCGAGTCC CTGAAGAAGA GAGGATGCAA CAGAGGAGGG AGATGCTACG AGATCCAGAA 
ATGAGAAATA AATTAATTTC TAACCCAACT AATTTTAACC ACATAGCACA CATGGGTCCT 
GGAGATGGAA TACAGATCCT AAAAGACCTG CCCATGCCAG GTTTCCCTTA TTCACCCCCT 
CATCACCACT CCGGCCTGAT CTCCTCTCCG AGCAACTTTG AGCACATCTA TCACATGACT 
GTCACTTCTG CTGAACAGTT TCTCTCTCCT GAGTCCATAA GCCCTGGGTC CCCCCCGTCC 
CTGCGCTCTG CCCCTGGCAC ACCACACTCT GTGACTCTGA GGAACCCACG GCCTCAGGAA 
AGCCGGACAG TATTCAGTGG CTCCGTCAGT ATTCCCTCCA TCACCAAGTC CCGCCCTGAG 
CCAGGCCGCT CCATGAGTGC CAGCAGCGGC CTGTCTGCAC GGTCGTCAGC CCAGAATGGC 
AGTGCACTAA AGAGGGAATT CTCTGGAGGA AGCTATAACA CAAAGCGACA GCCCATGCCC 
TCTCCCTCGG AGGGCTCTTT GTCATCAGGA GGCATGGACC AAGGAAGTGA CGCCCCTGCG 
AGGGACTATG ATGGAGAGGA CTCTGATTCT CCAAGGCATT CCACAGCTTC CAACAGCTCT 
AACCTGAGCA GCCCCCCAAG TCCCATTTCA CCCCAAAAGA CCAAGAGCCT CTCCCTGGAG 
AGCACAGACC GCGGGAGCTG GGATCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_017320130.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175619.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X11, mRNA.

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CloneID	OMu73308
Clone ID Related Accession (Same CDS sequence)	XM_017320130.2 , XM_011238803.3 , XM_011238803.2 , XM_017320130.1
Accession Version	XM_017320130.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320130.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGATG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGACTAACAG AATTGCACAC CCAAAAGCAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGCT GAGAGCTTAA GGCAGGAGCT GCGCAGAGCA 
GAAAGAGCCA AGAAAGAGCT GGAAGTTCAC ACCGAAGCTC TAATTGCTGA AGCATCAAAG 
GACAAGAAGC TCCGAGAGCA GAGCGAACAT TATTCTAAGC AACTGGAAAA CGAGTTGGAG 
GGCCTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAATAT GCAGCATAGA ACATCAGCAA 
GAGATAACTA AACTGAAAAC TGACTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAAATT 
TCTAAAAGAG AAGGAATTCA CGCAAGTGAG ATTAAAAACC TGAAGAAGGA GCTGCATGAC 
TCAGAAGGCC AGCAGCTTGC TCTCAACAAA GAGATTCTGG TTTTAAAAGA CAAATTGGAA 
AAAACCAGGA GAGAAAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAACAACAA 
TATGAACGGG AAAAAGTATT ATTAACTGAA GAGAATAAAA AGTTAACGAG TGAACTCGAT 
AAGCTGACTT CACTGTATGA GAGCTTAAGT TTGCGCAACC AGCACTTAGA AGAAGAAGTC 
AAAGATCTAG CAGATAAGAA GGAATCAGTT GCCCACTGGG AAGCCCAAAT CACAGAGATA 
ATTCAGTGGG TGAGCGATGA GAAGGATGCA CGAGGCTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AGTTGGAAGC ATTAAGAAAT TCCAGCTTGG GGACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCAAAACTG GACATGTCGG CTAGACTAGA GTTGCAGTCT 
GCTCTGGATG CAGAAATAAG AGCTAAGCAG GCCATCCAGG AAGAGCTGAA TAAAGTCAAA 
GCATCTAACA TCTTAACAGA ATGTAAACTG AAAGATTCAG AGAAGAAGAA CTTGGAACTG 
TTGTCCGAAA TCGAGCAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GACCAATTTG AAGATTCCCT TTCTTCTTCC TCATCCTCAC TGATTGATTT TTTGGATGAC 
CGCTCTCCGT CCTGTACTCC AGCTAGCAAA GGCAGACGTA TTGCAGACTG TGCTCCACTT 
CCAGCTCACA CACCAACCTT AAGGAAAAAG GGATGCCCGG CTTCAACTGG CTTTCCACCT 
AAGCGCAAGA CTCATCAATT TTTCGTGAAG TCTTTCACTG CCCCTACCAA GTGTCACCAG 
TGCACCTCCT TGATGGTCGG TTTGATAAGA CAGGGCTGTT CCTGTGAAGT CTGTGGGTTC 
TCATGTCATA TAACTTGTGT GAACAAAGCC CCGACGGTTT GCCCAGTTCC TCCTGAGCAA 
ACTAAAGGTC CACTGGGCAT AGACCCCCAG AAGGGAGTTG GGACGGCGTA TGAGGGTCAT 
GTCAGGATCC CTAAGCCGGC GGGAGTGAAG AAAGGGTGGC AGAGAGCACT GGCTGTGGTC 
TGCGACTTCA AACTGTTTCT TTATGATATC GCAGAGGGAA AAGCATCGCA GCCCACCTCC 
GTCATTAGTC AAGTGATTGA CATGAGAGAT GAAGAATTTT CTGTGAGTTC AGTCTTAGCT 
TCTGATGTTA TTCACGCAAG TCGAAAAGAT ATCCCCTGTA TATTCAGGGT CACAGCTTCC 
CAGCTCTCAG CACCTAGTAA CAAATGTTCC ATCCTGATGC TAGCAGACAG CGAGAATGAG 
AGGAGTAAGT GGGTGGGAGT GCTGAGTGAA CTCCACAAGA TTCTGAAGAA AAACAAGTTC 
AGAGACCGCT CAGTGTATGT CCCCAAGGAG GCGTACGACA GCACGCTACC CCTCATTAAG 
ACAACGCAGG CCGCTGCAAT CATAGATCAT GAACGGATAG CTTTGGGAAA TGAAGAAGGA 
TTATTTGTTG TGCATGTCAC CAAAGATGAG ATTGTTAGAG TTGGTGATAA TAAGAAAATC 
CATCAGATCG AACTCATTCC AAGTGACCAG CTTGTCGCTG TGATCTCAGG CCGAAATCGC 
CACGTCCGAC TTTTTCCTAT GTCAGCCTTG GATGGGCGAG AGACGGATTT TTATAAACTG 
GCAGAGACTA AAGGGTGTCA GACCATAGCT GCTGGGAAAG TGCGCCACGG AGCTCTGTCC 
TGCCTGTGTG TGGCCATGAA GAGACAGGTC CTCTGCTATG AACTGTTCCA AAGCAAGACC 
AGGCACAGGA AGTTTAAGGA GATTCAAGTC CCATGTAATG TCCAGTGGAT GGCCATCTTC 
AGTGAGCATC TCTGTGTGGG ATTCCAGTCA GGATTTCTCA GATACCCCCT GAATGGAGAA 
GGAGGTCCAT GCAATATGCT CCATTCAAAT GACCATACAC TGTCATTCAT TTCACACCAA 
CCAATGGACG CTCTCTGTGC AGTTGAGATC TCCAACAAAG AGTACTTGCT GTGTTTTAAC 
AGCATTGGGA TATACACAGA CTGCCAGGGC CGAAGATCCA GACAGCAGGA ACTGATGTGG 
CCAGCAAATC CTTCCTCCTG TTGTTACAAT GCTCCATATC TCTCCGTGTA TAGTGAAAAC 
GCAGTTGATA TCTTTGATGT AAACTCCATG GAATGGATAC AGACTCTTCC TCTCAAAAAG 
GTCCGACCTT TAAACACTGA AGGATCATTA AATCTCTTAG GATTGGAGAC CATTAGATTA 
ATATATTTCA AAAATAAGAT GGCAGAAGGG GATGAACTTG TGGTTCCTGA AACATCAGAT 
AATAGTCGGA AACAAATGGT TAGAAATATC AACAACAAAC GGCGTTACTC CTTCCGAGTC 
CCTGAAGAAG AGAGGATGCA ACAGAGGAGG GAGATGCTAC GAGATCCAGA AATGAGAAAT 
AAATTAATTT CTAACCCAAC TAATTTTAAC CACATAGCAC ACATGGGTCC TGGAGATGGA 
ATACAGATCC TAAAAGACCT GCCCATGCCA GGTTTCCCTT ATTCACCCCC TCATCACCAC 
TCCGGCCTGA TCTCCTCTCC GAGCAACTTT GAGCACATCT ATCACATGAC TGTCACTTCT 
GCTGAACAGT TTCTCTCTCC TGAGTCCATA AGCCCTGGGT CCCCCCCGTC CCTGCGCTCT 
GCCCCTGGCA CACCACACTC TGTGACTCTG AGGAACCCAC GGCCTCAGGA AAGCCGGACA 
GTATTCAGTG GCTCCGTCAG TATTCCCTCC ATCACCAAGT CCCGCCCTGA GCCAGGCCGC 
TCCATGAGTG CCAGCAGCGG CCTGTCTGCA CGGTCGTCAG CCCAGAATGG CAGTGCACTA 
AAGAGGGAAT TCTCTGGAGG AAGCTATAAC ACAAAGCGAC AGCCCATGCC CTCTCCCTCG 
GAGGGCTCTT TGTCATCAGG AGGCATGGAC CAAGGAAGTG ACGCCCCTGC GAGGGACTAT 
GATGGAGAGG ACTCTGATTC TCCAAGGCAT TCCACAGCTT CCAACAGCTC TAACCTGAGC 
AGCCCCCCAA GTCCCATTTC ACCCCAAAAG ACCAAGAGCC TCTCCCTGGA GAGCACAGAC 
CGCGGGAGCT GGGATCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175619.1
CDS	1575..6695
Translation	MKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSLSSSSSSLIDFLDDRSPSCTPASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_017320130.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320130.2

ORF Insert Sequence:

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGATG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGACTAACAG AATTGCACAC CCAAAAGCAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGCT GAGAGCTTAA GGCAGGAGCT GCGCAGAGCA 
GAAAGAGCCA AGAAAGAGCT GGAAGTTCAC ACCGAAGCTC TAATTGCTGA AGCATCAAAG 
GACAAGAAGC TCCGAGAGCA GAGCGAACAT TATTCTAAGC AACTGGAAAA CGAGTTGGAG 
GGCCTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAATAT GCAGCATAGA ACATCAGCAA 
GAGATAACTA AACTGAAAAC TGACTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAAATT 
TCTAAAAGAG AAGGAATTCA CGCAAGTGAG ATTAAAAACC TGAAGAAGGA GCTGCATGAC 
TCAGAAGGCC AGCAGCTTGC TCTCAACAAA GAGATTCTGG TTTTAAAAGA CAAATTGGAA 
AAAACCAGGA GAGAAAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAACAACAA 
TATGAACGGG AAAAAGTATT ATTAACTGAA GAGAATAAAA AGTTAACGAG TGAACTCGAT 
AAGCTGACTT CACTGTATGA GAGCTTAAGT TTGCGCAACC AGCACTTAGA AGAAGAAGTC 
AAAGATCTAG CAGATAAGAA GGAATCAGTT GCCCACTGGG AAGCCCAAAT CACAGAGATA 
ATTCAGTGGG TGAGCGATGA GAAGGATGCA CGAGGCTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AGTTGGAAGC ATTAAGAAAT TCCAGCTTGG GGACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCAAAACTG GACATGTCGG CTAGACTAGA GTTGCAGTCT 
GCTCTGGATG CAGAAATAAG AGCTAAGCAG GCCATCCAGG AAGAGCTGAA TAAAGTCAAA 
GCATCTAACA TCTTAACAGA ATGTAAACTG AAAGATTCAG AGAAGAAGAA CTTGGAACTG 
TTGTCCGAAA TCGAGCAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GACCAATTTG AAGATTCCCT TTCTTCTTCC TCATCCTCAC TGATTGATTT TTTGGATGAC 
CGCTCTCCGT CCTGTACTCC AGCTAGCAAA GGCAGACGTA TTGCAGACTG TGCTCCACTT 
CCAGCTCACA CACCAACCTT AAGGAAAAAG GGATGCCCGG CTTCAACTGG CTTTCCACCT 
AAGCGCAAGA CTCATCAATT TTTCGTGAAG TCTTTCACTG CCCCTACCAA GTGTCACCAG 
TGCACCTCCT TGATGGTCGG TTTGATAAGA CAGGGCTGTT CCTGTGAAGT CTGTGGGTTC 
TCATGTCATA TAACTTGTGT GAACAAAGCC CCGACGGTTT GCCCAGTTCC TCCTGAGCAA 
ACTAAAGGTC CACTGGGCAT AGACCCCCAG AAGGGAGTTG GGACGGCGTA TGAGGGTCAT 
GTCAGGATCC CTAAGCCGGC GGGAGTGAAG AAAGGGTGGC AGAGAGCACT GGCTGTGGTC 
TGCGACTTCA AACTGTTTCT TTATGATATC GCAGAGGGAA AAGCATCGCA GCCCACCTCC 
GTCATTAGTC AAGTGATTGA CATGAGAGAT GAAGAATTTT CTGTGAGTTC AGTCTTAGCT 
TCTGATGTTA TTCACGCAAG TCGAAAAGAT ATCCCCTGTA TATTCAGGGT CACAGCTTCC 
CAGCTCTCAG CACCTAGTAA CAAATGTTCC ATCCTGATGC TAGCAGACAG CGAGAATGAG 
AGGAGTAAGT GGGTGGGAGT GCTGAGTGAA CTCCACAAGA TTCTGAAGAA AAACAAGTTC 
AGAGACCGCT CAGTGTATGT CCCCAAGGAG GCGTACGACA GCACGCTACC CCTCATTAAG 
ACAACGCAGG CCGCTGCAAT CATAGATCAT GAACGGATAG CTTTGGGAAA TGAAGAAGGA 
TTATTTGTTG TGCATGTCAC CAAAGATGAG ATTGTTAGAG TTGGTGATAA TAAGAAAATC 
CATCAGATCG AACTCATTCC AAGTGACCAG CTTGTCGCTG TGATCTCAGG CCGAAATCGC 
CACGTCCGAC TTTTTCCTAT GTCAGCCTTG GATGGGCGAG AGACGGATTT TTATAAACTG 
GCAGAGACTA AAGGGTGTCA GACCATAGCT GCTGGGAAAG TGCGCCACGG AGCTCTGTCC 
TGCCTGTGTG TGGCCATGAA GAGACAGGTC CTCTGCTATG AACTGTTCCA AAGCAAGACC 
AGGCACAGGA AGTTTAAGGA GATTCAAGTC CCATGTAATG TCCAGTGGAT GGCCATCTTC 
AGTGAGCATC TCTGTGTGGG ATTCCAGTCA GGATTTCTCA GATACCCCCT GAATGGAGAA 
GGAGGTCCAT GCAATATGCT CCATTCAAAT GACCATACAC TGTCATTCAT TTCACACCAA 
CCAATGGACG CTCTCTGTGC AGTTGAGATC TCCAACAAAG AGTACTTGCT GTGTTTTAAC 
AGCATTGGGA TATACACAGA CTGCCAGGGC CGAAGATCCA GACAGCAGGA ACTGATGTGG 
CCAGCAAATC CTTCCTCCTG TTGTTACAAT GCTCCATATC TCTCCGTGTA TAGTGAAAAC 
GCAGTTGATA TCTTTGATGT AAACTCCATG GAATGGATAC AGACTCTTCC TCTCAAAAAG 
GTCCGACCTT TAAACACTGA AGGATCATTA AATCTCTTAG GATTGGAGAC CATTAGATTA 
ATATATTTCA AAAATAAGAT GGCAGAAGGG GATGAACTTG TGGTTCCTGA AACATCAGAT 
AATAGTCGGA AACAAATGGT TAGAAATATC AACAACAAAC GGCGTTACTC CTTCCGAGTC 
CCTGAAGAAG AGAGGATGCA ACAGAGGAGG GAGATGCTAC GAGATCCAGA AATGAGAAAT 
AAATTAATTT CTAACCCAAC TAATTTTAAC CACATAGCAC ACATGGGTCC TGGAGATGGA 
ATACAGATCC TAAAAGACCT GCCCATGCCA GGTTTCCCTT ATTCACCCCC TCATCACCAC 
TCCGGCCTGA TCTCCTCTCC GAGCAACTTT GAGCACATCT ATCACATGAC TGTCACTTCT 
GCTGAACAGT TTCTCTCTCC TGAGTCCATA AGCCCTGGGT CCCCCCCGTC CCTGCGCTCT 
GCCCCTGGCA CACCACACTC TGTGACTCTG AGGAACCCAC GGCCTCAGGA AAGCCGGACA 
GTATTCAGTG GCTCCGTCAG TATTCCCTCC ATCACCAAGT CCCGCCCTGA GCCAGGCCGC 
TCCATGAGTG CCAGCAGCGG CCTGTCTGCA CGGTCGTCAG CCCAGAATGG CAGTGCACTA 
AAGAGGGAAT TCTCTGGAGG AAGCTATAAC ACAAAGCGAC AGCCCATGCC CTCTCCCTCG 
GAGGGCTCTT TGTCATCAGG AGGCATGGAC CAAGGAAGTG ACGCCCCTGC GAGGGACTAT 
GATGGAGAGG ACTCTGATTC TCCAAGGCAT TCCACAGCTT CCAACAGCTC TAACCTGAGC 
AGCCCCCCAA GTCCCATTTC ACCCCAAAAG ACCAAGAGCC TCTCCCTGGA GAGCACAGAC 
CGCGGGAGCT GGGATCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_011238803.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237105.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X10, mRNA.

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CloneID	OMu73308
Clone ID Related Accession (Same CDS sequence)	XM_017320130.2 , XM_011238803.3 , XM_011238803.2 , XM_017320130.1
Accession Version	XM_011238803.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238803.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGATG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGACTAACAG AATTGCACAC CCAAAAGCAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGCT GAGAGCTTAA GGCAGGAGCT GCGCAGAGCA 
GAAAGAGCCA AGAAAGAGCT GGAAGTTCAC ACCGAAGCTC TAATTGCTGA AGCATCAAAG 
GACAAGAAGC TCCGAGAGCA GAGCGAACAT TATTCTAAGC AACTGGAAAA CGAGTTGGAG 
GGCCTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAATAT GCAGCATAGA ACATCAGCAA 
GAGATAACTA AACTGAAAAC TGACTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAAATT 
TCTAAAAGAG AAGGAATTCA CGCAAGTGAG ATTAAAAACC TGAAGAAGGA GCTGCATGAC 
TCAGAAGGCC AGCAGCTTGC TCTCAACAAA GAGATTCTGG TTTTAAAAGA CAAATTGGAA 
AAAACCAGGA GAGAAAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAACAACAA 
TATGAACGGG AAAAAGTATT ATTAACTGAA GAGAATAAAA AGTTAACGAG TGAACTCGAT 
AAGCTGACTT CACTGTATGA GAGCTTAAGT TTGCGCAACC AGCACTTAGA AGAAGAAGTC 
AAAGATCTAG CAGATAAGAA GGAATCAGTT GCCCACTGGG AAGCCCAAAT CACAGAGATA 
ATTCAGTGGG TGAGCGATGA GAAGGATGCA CGAGGCTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AGTTGGAAGC ATTAAGAAAT TCCAGCTTGG GGACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCAAAACTG GACATGTCGG CTAGACTAGA GTTGCAGTCT 
GCTCTGGATG CAGAAATAAG AGCTAAGCAG GCCATCCAGG AAGAGCTGAA TAAAGTCAAA 
GCATCTAACA TCTTAACAGA ATGTAAACTG AAAGATTCAG AGAAGAAGAA CTTGGAACTG 
TTGTCCGAAA TCGAGCAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GACCAATTTG AAGATTCCCT TTCTTCTTCC TCATCCTCAC TGATTGATTT TTTGGATGAC 
CGCTCTCCGT CCTGTACTCC AGCTAGCAAA GGCAGACGTA TTGCAGACTG TGCTCCACTT 
CCAGCTCACA CACCAACCTT AAGGAAAAAG GGATGCCCGG CTTCAACTGG CTTTCCACCT 
AAGCGCAAGA CTCATCAATT TTTCGTGAAG TCTTTCACTG CCCCTACCAA GTGTCACCAG 
TGCACCTCCT TGATGGTCGG TTTGATAAGA CAGGGCTGTT CCTGTGAAGT CTGTGGGTTC 
TCATGTCATA TAACTTGTGT GAACAAAGCC CCGACGGTTT GCCCAGTTCC TCCTGAGCAA 
ACTAAAGGTC CACTGGGCAT AGACCCCCAG AAGGGAGTTG GGACGGCGTA TGAGGGTCAT 
GTCAGGATCC CTAAGCCGGC GGGAGTGAAG AAAGGGTGGC AGAGAGCACT GGCTGTGGTC 
TGCGACTTCA AACTGTTTCT TTATGATATC GCAGAGGGAA AAGCATCGCA GCCCACCTCC 
GTCATTAGTC AAGTGATTGA CATGAGAGAT GAAGAATTTT CTGTGAGTTC AGTCTTAGCT 
TCTGATGTTA TTCACGCAAG TCGAAAAGAT ATCCCCTGTA TATTCAGGGT CACAGCTTCC 
CAGCTCTCAG CACCTAGTAA CAAATGTTCC ATCCTGATGC TAGCAGACAG CGAGAATGAG 
AGGAGTAAGT GGGTGGGAGT GCTGAGTGAA CTCCACAAGA TTCTGAAGAA AAACAAGTTC 
AGAGACCGCT CAGTGTATGT CCCCAAGGAG GCGTACGACA GCACGCTACC CCTCATTAAG 
ACAACGCAGG CCGCTGCAAT CATAGATCAT GAACGGATAG CTTTGGGAAA TGAAGAAGGA 
TTATTTGTTG TGCATGTCAC CAAAGATGAG ATTGTTAGAG TTGGTGATAA TAAGAAAATC 
CATCAGATCG AACTCATTCC AAGTGACCAG CTTGTCGCTG TGATCTCAGG CCGAAATCGC 
CACGTCCGAC TTTTTCCTAT GTCAGCCTTG GATGGGCGAG AGACGGATTT TTATAAACTG 
GCAGAGACTA AAGGGTGTCA GACCATAGCT GCTGGGAAAG TGCGCCACGG AGCTCTGTCC 
TGCCTGTGTG TGGCCATGAA GAGACAGGTC CTCTGCTATG AACTGTTCCA AAGCAAGACC 
AGGCACAGGA AGTTTAAGGA GATTCAAGTC CCATGTAATG TCCAGTGGAT GGCCATCTTC 
AGTGAGCATC TCTGTGTGGG ATTCCAGTCA GGATTTCTCA GATACCCCCT GAATGGAGAA 
GGAGGTCCAT GCAATATGCT CCATTCAAAT GACCATACAC TGTCATTCAT TTCACACCAA 
CCAATGGACG CTCTCTGTGC AGTTGAGATC TCCAACAAAG AGTACTTGCT GTGTTTTAAC 
AGCATTGGGA TATACACAGA CTGCCAGGGC CGAAGATCCA GACAGCAGGA ACTGATGTGG 
CCAGCAAATC CTTCCTCCTG TTGTTACAAT GCTCCATATC TCTCCGTGTA TAGTGAAAAC 
GCAGTTGATA TCTTTGATGT AAACTCCATG GAATGGATAC AGACTCTTCC TCTCAAAAAG 
GTCCGACCTT TAAACACTGA AGGATCATTA AATCTCTTAG GATTGGAGAC CATTAGATTA 
ATATATTTCA AAAATAAGAT GGCAGAAGGG GATGAACTTG TGGTTCCTGA AACATCAGAT 
AATAGTCGGA AACAAATGGT TAGAAATATC AACAACAAAC GGCGTTACTC CTTCCGAGTC 
CCTGAAGAAG AGAGGATGCA ACAGAGGAGG GAGATGCTAC GAGATCCAGA AATGAGAAAT 
AAATTAATTT CTAACCCAAC TAATTTTAAC CACATAGCAC ACATGGGTCC TGGAGATGGA 
ATACAGATCC TAAAAGACCT GCCCATGCCA GGTTTCCCTT ATTCACCCCC TCATCACCAC 
TCCGGCCTGA TCTCCTCTCC GAGCAACTTT GAGCACATCT ATCACATGAC TGTCACTTCT 
GCTGAACAGT TTCTCTCTCC TGAGTCCATA AGCCCTGGGT CCCCCCCGTC CCTGCGCTCT 
GCCCCTGGCA CACCACACTC TGTGACTCTG AGGAACCCAC GGCCTCAGGA AAGCCGGACA 
GTATTCAGTG GCTCCGTCAG TATTCCCTCC ATCACCAAGT CCCGCCCTGA GCCAGGCCGC 
TCCATGAGTG CCAGCAGCGG CCTGTCTGCA CGGTCGTCAG CCCAGAATGG CAGTGCACTA 
AAGAGGGAAT TCTCTGGAGG AAGCTATAAC ACAAAGCGAC AGCCCATGCC CTCTCCCTCG 
GAGGGCTCTT TGTCATCAGG AGGCATGGAC CAAGGAAGTG ACGCCCCTGC GAGGGACTAT 
GATGGAGAGG ACTCTGATTC TCCAAGGCAT TCCACAGCTT CCAACAGCTC TAACCTGAGC 
AGCCCCCCAA GTCCCATTTC ACCCCAAAAG ACCAAGAGCC TCTCCCTGGA GAGCACAGAC 
CGCGGGAGCT GGGATCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237105.1
CDS	1502..6622
Translation	MKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSLSSSSSSLIDFLDDRSPSCTPASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_011238803.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238803.3

ORF Insert Sequence:

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGATG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGACTAACAG AATTGCACAC CCAAAAGCAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGCT GAGAGCTTAA GGCAGGAGCT GCGCAGAGCA 
GAAAGAGCCA AGAAAGAGCT GGAAGTTCAC ACCGAAGCTC TAATTGCTGA AGCATCAAAG 
GACAAGAAGC TCCGAGAGCA GAGCGAACAT TATTCTAAGC AACTGGAAAA CGAGTTGGAG 
GGCCTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAATAT GCAGCATAGA ACATCAGCAA 
GAGATAACTA AACTGAAAAC TGACTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAAATT 
TCTAAAAGAG AAGGAATTCA CGCAAGTGAG ATTAAAAACC TGAAGAAGGA GCTGCATGAC 
TCAGAAGGCC AGCAGCTTGC TCTCAACAAA GAGATTCTGG TTTTAAAAGA CAAATTGGAA 
AAAACCAGGA GAGAAAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAACAACAA 
TATGAACGGG AAAAAGTATT ATTAACTGAA GAGAATAAAA AGTTAACGAG TGAACTCGAT 
AAGCTGACTT CACTGTATGA GAGCTTAAGT TTGCGCAACC AGCACTTAGA AGAAGAAGTC 
AAAGATCTAG CAGATAAGAA GGAATCAGTT GCCCACTGGG AAGCCCAAAT CACAGAGATA 
ATTCAGTGGG TGAGCGATGA GAAGGATGCA CGAGGCTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AGTTGGAAGC ATTAAGAAAT TCCAGCTTGG GGACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCAAAACTG GACATGTCGG CTAGACTAGA GTTGCAGTCT 
GCTCTGGATG CAGAAATAAG AGCTAAGCAG GCCATCCAGG AAGAGCTGAA TAAAGTCAAA 
GCATCTAACA TCTTAACAGA ATGTAAACTG AAAGATTCAG AGAAGAAGAA CTTGGAACTG 
TTGTCCGAAA TCGAGCAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GACCAATTTG AAGATTCCCT TTCTTCTTCC TCATCCTCAC TGATTGATTT TTTGGATGAC 
CGCTCTCCGT CCTGTACTCC AGCTAGCAAA GGCAGACGTA TTGCAGACTG TGCTCCACTT 
CCAGCTCACA CACCAACCTT AAGGAAAAAG GGATGCCCGG CTTCAACTGG CTTTCCACCT 
AAGCGCAAGA CTCATCAATT TTTCGTGAAG TCTTTCACTG CCCCTACCAA GTGTCACCAG 
TGCACCTCCT TGATGGTCGG TTTGATAAGA CAGGGCTGTT CCTGTGAAGT CTGTGGGTTC 
TCATGTCATA TAACTTGTGT GAACAAAGCC CCGACGGTTT GCCCAGTTCC TCCTGAGCAA 
ACTAAAGGTC CACTGGGCAT AGACCCCCAG AAGGGAGTTG GGACGGCGTA TGAGGGTCAT 
GTCAGGATCC CTAAGCCGGC GGGAGTGAAG AAAGGGTGGC AGAGAGCACT GGCTGTGGTC 
TGCGACTTCA AACTGTTTCT TTATGATATC GCAGAGGGAA AAGCATCGCA GCCCACCTCC 
GTCATTAGTC AAGTGATTGA CATGAGAGAT GAAGAATTTT CTGTGAGTTC AGTCTTAGCT 
TCTGATGTTA TTCACGCAAG TCGAAAAGAT ATCCCCTGTA TATTCAGGGT CACAGCTTCC 
CAGCTCTCAG CACCTAGTAA CAAATGTTCC ATCCTGATGC TAGCAGACAG CGAGAATGAG 
AGGAGTAAGT GGGTGGGAGT GCTGAGTGAA CTCCACAAGA TTCTGAAGAA AAACAAGTTC 
AGAGACCGCT CAGTGTATGT CCCCAAGGAG GCGTACGACA GCACGCTACC CCTCATTAAG 
ACAACGCAGG CCGCTGCAAT CATAGATCAT GAACGGATAG CTTTGGGAAA TGAAGAAGGA 
TTATTTGTTG TGCATGTCAC CAAAGATGAG ATTGTTAGAG TTGGTGATAA TAAGAAAATC 
CATCAGATCG AACTCATTCC AAGTGACCAG CTTGTCGCTG TGATCTCAGG CCGAAATCGC 
CACGTCCGAC TTTTTCCTAT GTCAGCCTTG GATGGGCGAG AGACGGATTT TTATAAACTG 
GCAGAGACTA AAGGGTGTCA GACCATAGCT GCTGGGAAAG TGCGCCACGG AGCTCTGTCC 
TGCCTGTGTG TGGCCATGAA GAGACAGGTC CTCTGCTATG AACTGTTCCA AAGCAAGACC 
AGGCACAGGA AGTTTAAGGA GATTCAAGTC CCATGTAATG TCCAGTGGAT GGCCATCTTC 
AGTGAGCATC TCTGTGTGGG ATTCCAGTCA GGATTTCTCA GATACCCCCT GAATGGAGAA 
GGAGGTCCAT GCAATATGCT CCATTCAAAT GACCATACAC TGTCATTCAT TTCACACCAA 
CCAATGGACG CTCTCTGTGC AGTTGAGATC TCCAACAAAG AGTACTTGCT GTGTTTTAAC 
AGCATTGGGA TATACACAGA CTGCCAGGGC CGAAGATCCA GACAGCAGGA ACTGATGTGG 
CCAGCAAATC CTTCCTCCTG TTGTTACAAT GCTCCATATC TCTCCGTGTA TAGTGAAAAC 
GCAGTTGATA TCTTTGATGT AAACTCCATG GAATGGATAC AGACTCTTCC TCTCAAAAAG 
GTCCGACCTT TAAACACTGA AGGATCATTA AATCTCTTAG GATTGGAGAC CATTAGATTA 
ATATATTTCA AAAATAAGAT GGCAGAAGGG GATGAACTTG TGGTTCCTGA AACATCAGAT 
AATAGTCGGA AACAAATGGT TAGAAATATC AACAACAAAC GGCGTTACTC CTTCCGAGTC 
CCTGAAGAAG AGAGGATGCA ACAGAGGAGG GAGATGCTAC GAGATCCAGA AATGAGAAAT 
AAATTAATTT CTAACCCAAC TAATTTTAAC CACATAGCAC ACATGGGTCC TGGAGATGGA 
ATACAGATCC TAAAAGACCT GCCCATGCCA GGTTTCCCTT ATTCACCCCC TCATCACCAC 
TCCGGCCTGA TCTCCTCTCC GAGCAACTTT GAGCACATCT ATCACATGAC TGTCACTTCT 
GCTGAACAGT TTCTCTCTCC TGAGTCCATA AGCCCTGGGT CCCCCCCGTC CCTGCGCTCT 
GCCCCTGGCA CACCACACTC TGTGACTCTG AGGAACCCAC GGCCTCAGGA AAGCCGGACA 
GTATTCAGTG GCTCCGTCAG TATTCCCTCC ATCACCAAGT CCCGCCCTGA GCCAGGCCGC 
TCCATGAGTG CCAGCAGCGG CCTGTCTGCA CGGTCGTCAG CCCAGAATGG CAGTGCACTA 
AAGAGGGAAT TCTCTGGAGG AAGCTATAAC ACAAAGCGAC AGCCCATGCC CTCTCCCTCG 
GAGGGCTCTT TGTCATCAGG AGGCATGGAC CAAGGAAGTG ACGCCCCTGC GAGGGACTAT 
GATGGAGAGG ACTCTGATTC TCCAAGGCAT TCCACAGCTT CCAACAGCTC TAACCTGAGC 
AGCCCCCCAA GTCCCATTTC ACCCCAAAAG ACCAAGAGCC TCTCCCTGGA GAGCACAGAC 
CGCGGGAGCT GGGATCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_011238804.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237106.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X14, mRNA.

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CloneID	OMu73309
Clone ID Related Accession (Same CDS sequence)	XM_011238804.3 , XM_011238804.2
Accession Version	XM_011238804.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238804.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGCTGGA AGTTCACACC GAAGCTCTAA TTGCTGAAGC ATCAAAGGAC 
AAGAAGCTCC GAGAGCAGAG CGAACATTAT TCTAAGCAAC TGGAAAACGA GTTGGAGGGC 
CTGAAGCAAA AACAAATTAG TTACTCACCA GGAATATGCA GCATAGAACA TCAGCAAGAG 
ATAACTAAAC TGAAAACTGA CTTGGAAAAG AAAAGTATCT TTTATGAAGA AGAAATTTCT 
AAAAGAGAAG GAATTCACGC AAGTGAGATT AAAAACCTGA AGAAGGAGCT GCATGACTCA 
GAAGGCCAGC AGCTTGCTCT CAACAAAGAG ATTCTGGTTT TAAAAGACAA ATTGGAAAAA 
ACCAGGAGAG AAAGTCAAAG TGAAAGGGAA GAATTTGAAA ATGAGTTCAA ACAACAATAT 
GAACGGGAAA AAGTATTATT AACTGAAGAG AATAAAAAGT TAACGAGTGA ACTCGATAAG 
CTGACTTCAC TGTATGAGAG CTTAAGTTTG CGCAACCAGC ACTTAGAAGA AGAAGTCAAA 
GATCTAGCAG ATAAGAAGGA ATCAGTTGCC CACTGGGAAG CCCAAATCAC AGAGATAATT 
CAGTGGGTGA GCGATGAGAA GGATGCACGA GGCTATCTTC AGGCCTTAGC TTCTAAAATG 
ACTGAAGAGT TGGAAGCATT AAGAAATTCC AGCTTGGGGA CACGAGCAAC AGATATGCCC 
TGGAAAATGC GTCGTTTTGC AAAACTGGAC ATGTCGGCTA GACTAGAGTT GCAGTCTGCT 
CTGGATGCAG AAATAAGAGC TAAGCAGGCC ATCCAGGAAG AGCTGAATAA AGTCAAAGCA 
TCTAACATCT TAACAGAATG TAAACTGAAA GATTCAGAGA AGAAGAACTT GGAACTGTTG 
TCCGAAATCG AGCAGCTGAT AAAGGACACT GAAGAGCTTA GATCTGAAAA GGGTATAGAG 
CACCAAGACT CACAGCATTC TTTCTTGGCA TTTTTGAATA CGCCTACCGA TGCTCTGGAC 
CAATTTGAAG ATTCCCTTTC TTCTTCCTCA TCCTCACTGA TTGATTTTTT GGATGACCGC 
TCTCCGTCCT GTACTCCAGC TAGCAAAGGC AGACGTATTG CAGACTGTGC TCCACTTCCA 
GCTCACACAC CAACCTTAAG GAAAAAGGGA TGCCCGGCTT CAACTGGCTT TCCACCTAAG 
CGCAAGACTC ATCAATTTTT CGTGAAGTCT TTCACTGCCC CTACCAAGTG TCACCAGTGC 
ACCTCCTTGA TGGTCGGTTT GATAAGACAG GGCTGTTCCT GTGAAGTCTG TGGGTTCTCA 
TGTCATATAA CTTGTGTGAA CAAAGCCCCG ACGGTTTGCC CAGTTCCTCC TGAGCAAACT 
AAAGGTCCAC TGGGCATAGA CCCCCAGAAG GGAGTTGGGA CGGCGTATGA GGGTCATGTC 
AGGATCCCTA AGCCGGCGGG AGTGAAGAAA GGGTGGCAGA GAGCACTGGC TGTGGTCTGC 
GACTTCAAAC TGTTTCTTTA TGATATCGCA GAGGGAAAAG CATCGCAGCC CACCTCCGTC 
ATTAGTCAAG TGATTGACAT GAGAGATGAA GAATTTTCTG TGAGTTCAGT CTTAGCTTCT 
GATGTTATTC ACGCAAGTCG AAAAGATATC CCCTGTATAT TCAGGGTCAC AGCTTCCCAG 
CTCTCAGCAC CTAGTAACAA ATGTTCCATC CTGATGCTAG CAGACAGCGA GAATGAGAGG 
AGTAAGTGGG TGGGAGTGCT GAGTGAACTC CACAAGATTC TGAAGAAAAA CAAGTTCAGA 
GACCGCTCAG TGTATGTCCC CAAGGAGGCG TACGACAGCA CGCTACCCCT CATTAAGACA 
ACGCAGGCCG CTGCAATCAT AGATCATGAA CGGATAGCTT TGGGAAATGA AGAAGGATTA 
TTTGTTGTGC ATGTCACCAA AGATGAGATT GTTAGAGTTG GTGATAATAA GAAAATCCAT 
CAGATCGAAC TCATTCCAAG TGACCAGCTT GTCGCTGTGA TCTCAGGCCG AAATCGCCAC 
GTCCGACTTT TTCCTATGTC AGCCTTGGAT GGGCGAGAGA CGGATTTTTA TAAACTGGCA 
GAGACTAAAG GGTGTCAGAC CATAGCTGCT GGGAAAGTGC GCCACGGAGC TCTGTCCTGC 
CTGTGTGTGG CCATGAAGAG ACAGGTCCTC TGCTATGAAC TGTTCCAAAG CAAGACCAGG 
CACAGGAAGT TTAAGGAGAT TCAAGTCCCA TGTAATGTCC AGTGGATGGC CATCTTCAGT 
GAGCATCTCT GTGTGGGATT CCAGTCAGGA TTTCTCAGAT ACCCCCTGAA TGGAGAAGGA 
GGTCCATGCA ATATGCTCCA TTCAAATGAC CATACACTGT CATTCATTTC ACACCAACCA 
ATGGACGCTC TCTGTGCAGT TGAGATCTCC AACAAAGAGT ACTTGCTGTG TTTTAACAGC 
ATTGGGATAT ACACAGACTG CCAGGGCCGA AGATCCAGAC AGCAGGAACT GATGTGGCCA 
GCAAATCCTT CCTCCTGTTG TTACAATGCT CCATATCTCT CCGTGTATAG TGAAAACGCA 
GTTGATATCT TTGATGTAAA CTCCATGGAA TGGATACAGA CTCTTCCTCT CAAAAAGGTC 
CGACCTTTAA ACACTGAAGG ATCATTAAAT CTCTTAGGAT TGGAGACCAT TAGATTAATA 
TATTTCAAAA ATAAGATGGC AGAAGGGGAT GAACTTGTGG TTCCTGAAAC ATCAGATAAT 
AGTCGGAAAC AAATGGTTAG AAATATCAAC AACAAACGGC GTTACTCCTT CCGAGTCCCT 
GAAGAAGAGA GGATGCAACA GAGGAGGGAG ATGCTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ACCCAACTAA TTTTAACCAC ATAGCACACA TGGGTCCTGG AGATGGAATA 
CAGATCCTAA AAGACCTGCC CATGCCAGGT TTCCCTTATT CACCCCCTCA TCACCACTCC 
GGCCTGATCT CCTCTCCGAG CAACTTTGAG CACATCTATC ACATGACTGT CACTTCTGCT 
GAACAGTTTC TCTCTCCTGA GTCCATAAGC CCTGGGTCCC CCCCGTCCCT GCGCTCTGCC 
CCTGGCACAC CACACTCTGT GACTCTGAGG AACCCACGGC CTCAGGAAAG CCGGACAGTA 
TTCAGTGGCT CCGTCAGTAT TCCCTCCATC ACCAAGTCCC GCCCTGAGCC AGGCCGCTCC 
ATGAGTGCCA GCAGCGGCCT GTCTGCACGG TCGTCAGCCC AGAATGGCAG TGCACTAAAG 
AGGGAATTCT CTGGAGGAAG CTATAACACA AAGCGACAGC CCATGCCCTC TCCCTCGGAG 
GGCTCTTTGT CATCAGGAGG CATGGACCAA GGAAGTGACG CCCCTGCGAG GGACTATGAT 
GGAGAGGACT CTGATTCTCC AAGGCATTCC ACAGCTTCCA ACAGCTCTAA CCTGAGCAGC 
CCCCCAAGTC CCATTTCACC CCAAAAGACC AAGAGCCTCT CCCTGGAGAG CACAGACCGC 
GGGAGCTGGG ATCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237106.1
CDS	1502..6379
Translation	MKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKLEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSLSSSSSSLIDFLDDRSPSCTPASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_011238804.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238804.3

ORF Insert Sequence:

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGCTGGA AGTTCACACC GAAGCTCTAA TTGCTGAAGC ATCAAAGGAC 
AAGAAGCTCC GAGAGCAGAG CGAACATTAT TCTAAGCAAC TGGAAAACGA GTTGGAGGGC 
CTGAAGCAAA AACAAATTAG TTACTCACCA GGAATATGCA GCATAGAACA TCAGCAAGAG 
ATAACTAAAC TGAAAACTGA CTTGGAAAAG AAAAGTATCT TTTATGAAGA AGAAATTTCT 
AAAAGAGAAG GAATTCACGC AAGTGAGATT AAAAACCTGA AGAAGGAGCT GCATGACTCA 
GAAGGCCAGC AGCTTGCTCT CAACAAAGAG ATTCTGGTTT TAAAAGACAA ATTGGAAAAA 
ACCAGGAGAG AAAGTCAAAG TGAAAGGGAA GAATTTGAAA ATGAGTTCAA ACAACAATAT 
GAACGGGAAA AAGTATTATT AACTGAAGAG AATAAAAAGT TAACGAGTGA ACTCGATAAG 
CTGACTTCAC TGTATGAGAG CTTAAGTTTG CGCAACCAGC ACTTAGAAGA AGAAGTCAAA 
GATCTAGCAG ATAAGAAGGA ATCAGTTGCC CACTGGGAAG CCCAAATCAC AGAGATAATT 
CAGTGGGTGA GCGATGAGAA GGATGCACGA GGCTATCTTC AGGCCTTAGC TTCTAAAATG 
ACTGAAGAGT TGGAAGCATT AAGAAATTCC AGCTTGGGGA CACGAGCAAC AGATATGCCC 
TGGAAAATGC GTCGTTTTGC AAAACTGGAC ATGTCGGCTA GACTAGAGTT GCAGTCTGCT 
CTGGATGCAG AAATAAGAGC TAAGCAGGCC ATCCAGGAAG AGCTGAATAA AGTCAAAGCA 
TCTAACATCT TAACAGAATG TAAACTGAAA GATTCAGAGA AGAAGAACTT GGAACTGTTG 
TCCGAAATCG AGCAGCTGAT AAAGGACACT GAAGAGCTTA GATCTGAAAA GGGTATAGAG 
CACCAAGACT CACAGCATTC TTTCTTGGCA TTTTTGAATA CGCCTACCGA TGCTCTGGAC 
CAATTTGAAG ATTCCCTTTC TTCTTCCTCA TCCTCACTGA TTGATTTTTT GGATGACCGC 
TCTCCGTCCT GTACTCCAGC TAGCAAAGGC AGACGTATTG CAGACTGTGC TCCACTTCCA 
GCTCACACAC CAACCTTAAG GAAAAAGGGA TGCCCGGCTT CAACTGGCTT TCCACCTAAG 
CGCAAGACTC ATCAATTTTT CGTGAAGTCT TTCACTGCCC CTACCAAGTG TCACCAGTGC 
ACCTCCTTGA TGGTCGGTTT GATAAGACAG GGCTGTTCCT GTGAAGTCTG TGGGTTCTCA 
TGTCATATAA CTTGTGTGAA CAAAGCCCCG ACGGTTTGCC CAGTTCCTCC TGAGCAAACT 
AAAGGTCCAC TGGGCATAGA CCCCCAGAAG GGAGTTGGGA CGGCGTATGA GGGTCATGTC 
AGGATCCCTA AGCCGGCGGG AGTGAAGAAA GGGTGGCAGA GAGCACTGGC TGTGGTCTGC 
GACTTCAAAC TGTTTCTTTA TGATATCGCA GAGGGAAAAG CATCGCAGCC CACCTCCGTC 
ATTAGTCAAG TGATTGACAT GAGAGATGAA GAATTTTCTG TGAGTTCAGT CTTAGCTTCT 
GATGTTATTC ACGCAAGTCG AAAAGATATC CCCTGTATAT TCAGGGTCAC AGCTTCCCAG 
CTCTCAGCAC CTAGTAACAA ATGTTCCATC CTGATGCTAG CAGACAGCGA GAATGAGAGG 
AGTAAGTGGG TGGGAGTGCT GAGTGAACTC CACAAGATTC TGAAGAAAAA CAAGTTCAGA 
GACCGCTCAG TGTATGTCCC CAAGGAGGCG TACGACAGCA CGCTACCCCT CATTAAGACA 
ACGCAGGCCG CTGCAATCAT AGATCATGAA CGGATAGCTT TGGGAAATGA AGAAGGATTA 
TTTGTTGTGC ATGTCACCAA AGATGAGATT GTTAGAGTTG GTGATAATAA GAAAATCCAT 
CAGATCGAAC TCATTCCAAG TGACCAGCTT GTCGCTGTGA TCTCAGGCCG AAATCGCCAC 
GTCCGACTTT TTCCTATGTC AGCCTTGGAT GGGCGAGAGA CGGATTTTTA TAAACTGGCA 
GAGACTAAAG GGTGTCAGAC CATAGCTGCT GGGAAAGTGC GCCACGGAGC TCTGTCCTGC 
CTGTGTGTGG CCATGAAGAG ACAGGTCCTC TGCTATGAAC TGTTCCAAAG CAAGACCAGG 
CACAGGAAGT TTAAGGAGAT TCAAGTCCCA TGTAATGTCC AGTGGATGGC CATCTTCAGT 
GAGCATCTCT GTGTGGGATT CCAGTCAGGA TTTCTCAGAT ACCCCCTGAA TGGAGAAGGA 
GGTCCATGCA ATATGCTCCA TTCAAATGAC CATACACTGT CATTCATTTC ACACCAACCA 
ATGGACGCTC TCTGTGCAGT TGAGATCTCC AACAAAGAGT ACTTGCTGTG TTTTAACAGC 
ATTGGGATAT ACACAGACTG CCAGGGCCGA AGATCCAGAC AGCAGGAACT GATGTGGCCA 
GCAAATCCTT CCTCCTGTTG TTACAATGCT CCATATCTCT CCGTGTATAG TGAAAACGCA 
GTTGATATCT TTGATGTAAA CTCCATGGAA TGGATACAGA CTCTTCCTCT CAAAAAGGTC 
CGACCTTTAA ACACTGAAGG ATCATTAAAT CTCTTAGGAT TGGAGACCAT TAGATTAATA 
TATTTCAAAA ATAAGATGGC AGAAGGGGAT GAACTTGTGG TTCCTGAAAC ATCAGATAAT 
AGTCGGAAAC AAATGGTTAG AAATATCAAC AACAAACGGC GTTACTCCTT CCGAGTCCCT 
GAAGAAGAGA GGATGCAACA GAGGAGGGAG ATGCTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ACCCAACTAA TTTTAACCAC ATAGCACACA TGGGTCCTGG AGATGGAATA 
CAGATCCTAA AAGACCTGCC CATGCCAGGT TTCCCTTATT CACCCCCTCA TCACCACTCC 
GGCCTGATCT CCTCTCCGAG CAACTTTGAG CACATCTATC ACATGACTGT CACTTCTGCT 
GAACAGTTTC TCTCTCCTGA GTCCATAAGC CCTGGGTCCC CCCCGTCCCT GCGCTCTGCC 
CCTGGCACAC CACACTCTGT GACTCTGAGG AACCCACGGC CTCAGGAAAG CCGGACAGTA 
TTCAGTGGCT CCGTCAGTAT TCCCTCCATC ACCAAGTCCC GCCCTGAGCC AGGCCGCTCC 
ATGAGTGCCA GCAGCGGCCT GTCTGCACGG TCGTCAGCCC AGAATGGCAG TGCACTAAAG 
AGGGAATTCT CTGGAGGAAG CTATAACACA AAGCGACAGC CCATGCCCTC TCCCTCGGAG 
GGCTCTTTGT CATCAGGAGG CATGGACCAA GGAAGTGACG CCCCTGCGAG GGACTATGAT 
GGAGAGGACT CTGATTCTCC AAGGCATTCC ACAGCTTCCA ACAGCTCTAA CCTGAGCAGC 
CCCCCAAGTC CCATTTCACC CCAAAAGACC AAGAGCCTCT CCCTGGAGAG CACAGACCGC 
GGGAGCTGGG ATCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_017320127.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175616.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMu77094
Clone ID Related Accession (Same CDS sequence)	XM_017320127.1 , NM_001359543.1
Accession Version	XM_017320127.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAAGATTCC CTTTCTTCTT CCTCATCCTC ACTGATTGAT 
TTTTTGGATG ACATTGCAGA CTGTGCTCCA CTTCCAGCTC ACACACCAAC CTTAAGGAAA 
AAGGGATGCC CGGCTTCAAC TGGCTTTCCA CCTAAGCGCA AGACTCATCA ATTTTTCGTG 
AAGTCTTTCA CTGCCCCTAC CAAGTGTCAC CAGTGCACCT CCTTGATGGT CGGTTTGATA 
AGACAGGGCT GTTCCTGTGA AGTCTGTGGG TTCTCATGTC ATATAACTTG TGTGAACAAA 
GCCCCGACGG TTTGCCCAGT TCCTCCTGAG CAAACTAAAG GTCCACTGGG CATAGACCCC 
CAGAAGGGAG TTGGGACGGC GTATGAGGGT CATGTCAGGA TCCCTAAGCC GGCGGGAGTG 
AAGAAAGGGT GGCAGAGAGC ACTGGCTGTG GTCTGCGACT TCAAACTGTT TCTTTATGAT 
ATCGCAGAGG GAAAAGCATC GCAGCCCACC TCCGTCATTA GTCAAGTGAT TGACATGAGA 
GATGAAGAAT TTTCTGTGAG TTCAGTCTTA GCTTCTGATG TTATTCACGC AAGTCGAAAA 
GATATCCCCT GTATATTCAG GGTCACAGCT TCCCAGCTCT CAGCACCTAG TAACAAATGT 
TCCATCCTGA TGCTAGCAGA CAGCGAGAAT GAGAGGAGTA AGTGGGTGGG AGTGCTGAGT 
GAACTCCACA AGATTCTGAA GAAAAACAAG TTCAGAGACC GCTCAGTGTA TGTCCCCAAG 
GAGGCGTACG ACAGCACGCT ACCCCTCATT AAGACAACGC AGGCCGCTGC AATCATAGAT 
CATGAACGGA TAGCTTTGGG AAATGAAGAA GGATTATTTG TTGTGCATGT CACCAAAGAT 
GAGATTGTTA GAGTTGGTGA TAATAAGAAA ATCCATCAGA TCGAACTCAT TCCAAGTGAC 
CAGCTTGTCG CTGTGATCTC AGGCCGAAAT CGCCACGTCC GACTTTTTCC TATGTCAGCC 
TTGGATGGGC GAGAGACGGA TTTTTATAAA CTGGCAGAGA CTAAAGGGTG TCAGACCATA 
GCTGCTGGGA AAGTGCGCCA CGGAGCTCTG TCCTGCCTGT GTGTGGCCAT GAAGAGACAG 
GTCCTCTGCT ATGAACTGTT CCAAAGCAAG ACCAGGCACA GGAAGTTTAA GGAGATTCAA 
GTCCCATGTA ATGTCCAGTG GATGGCCATC TTCAGTGAGC ATCTCTGTGT GGGATTCCAG 
TCAGGATTTC TCAGATACCC CCTGAATGGA GAAGGAGGTC CATGCAATAT GCTCCATTCA 
AATGACCATA CACTGTCATT CATTTCACAC CAACCAATGG ACGCTCTCTG TGCAGTTGAG 
ATCTCCAACA AAGAGTACTT GCTGTGTTTT AACAGCATTG GGATATACAC AGACTGCCAG 
GGCCGAAGAT CCAGACAGCA GGAACTGATG TGGCCAGCAA ATCCTTCCTC CTGTTGTTAC 
AATGCTCCAT ATCTCTCCGT GTATAGTGAA AACGCAGTTG ATATCTTTGA TGTAAACTCC 
ATGGAATGGA TACAGACTCT TCCTCTCAAA AAGGTCCGAC CTTTAAACAC TGAAGGATCA 
TTAAATCTCT TAGGATTGGA GACCATTAGA TTAATATATT TCAAAAATAA GATGGCAGAA 
GGGGATGAAC TTGTGGTTCC TGAAACATCA GATAATAGTC GGAAACAAAT GGTTAGAAAT 
ATCAACAACA AACGGCGTTA CTCCTTCCGA GTCCCTGAAG AAGAGAGGAT GCAACAGAGG 
AGGGAGATGC TACGAGATCC AGAAATGAGA AATAAATTAA TTTCTAACCC AACTAATTTT 
AACCACATAG CACACATGGG TCCTGGAGAT GGAATACAGA TCCTAAAAGA CCTGCCCATG 
AACCCACGGC CTCAGGAAAG CCGGACAGTA TTCAGTGGCT CCGTCAGTAT TCCCTCCATC 
ACCAAGTCCC GCCCTGAGCC AGGCCGCTCC ATGAGTGCCA GCAGCGGCCT GTCTGCACGG 
TCGTCAGCCC AGAATGGCAG TGCACTAAAG AGGGAATTCT CTGGAGGAAG CTATAACACA 
AAGCGACAGC CCATGCCCTC TCCCTCGGAG GGCTCTTTGT CATCAGGAGG CATGGACCAA 
GGAAGTGACG CCCCTGCGAG GGACTATGAT GGAGAGGACT CTGATTCTCC AAGGCATTCC 
ACAGCTTCCA ACAGCTCTAA CCTGAGCAGC CCCCCAAGTC CCATTTCACC CCAAAAGACC 
AAGAGCCTCT CCCTGGAGAG CACAGACCGC GGGAGCTGGG ATCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175616.1
CDS	1142..6349
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSLSSSSSSLIDFLDDIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_017320127.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320127.1

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAAGATTCC CTTTCTTCTT CCTCATCCTC ACTGATTGAT 
TTTTTGGATG ACATTGCAGA CTGTGCTCCA CTTCCAGCTC ACACACCAAC CTTAAGGAAA 
AAGGGATGCC CGGCTTCAAC TGGCTTTCCA CCTAAGCGCA AGACTCATCA ATTTTTCGTG 
AAGTCTTTCA CTGCCCCTAC CAAGTGTCAC CAGTGCACCT CCTTGATGGT CGGTTTGATA 
AGACAGGGCT GTTCCTGTGA AGTCTGTGGG TTCTCATGTC ATATAACTTG TGTGAACAAA 
GCCCCGACGG TTTGCCCAGT TCCTCCTGAG CAAACTAAAG GTCCACTGGG CATAGACCCC 
CAGAAGGGAG TTGGGACGGC GTATGAGGGT CATGTCAGGA TCCCTAAGCC GGCGGGAGTG 
AAGAAAGGGT GGCAGAGAGC ACTGGCTGTG GTCTGCGACT TCAAACTGTT TCTTTATGAT 
ATCGCAGAGG GAAAAGCATC GCAGCCCACC TCCGTCATTA GTCAAGTGAT TGACATGAGA 
GATGAAGAAT TTTCTGTGAG TTCAGTCTTA GCTTCTGATG TTATTCACGC AAGTCGAAAA 
GATATCCCCT GTATATTCAG GGTCACAGCT TCCCAGCTCT CAGCACCTAG TAACAAATGT 
TCCATCCTGA TGCTAGCAGA CAGCGAGAAT GAGAGGAGTA AGTGGGTGGG AGTGCTGAGT 
GAACTCCACA AGATTCTGAA GAAAAACAAG TTCAGAGACC GCTCAGTGTA TGTCCCCAAG 
GAGGCGTACG ACAGCACGCT ACCCCTCATT AAGACAACGC AGGCCGCTGC AATCATAGAT 
CATGAACGGA TAGCTTTGGG AAATGAAGAA GGATTATTTG TTGTGCATGT CACCAAAGAT 
GAGATTGTTA GAGTTGGTGA TAATAAGAAA ATCCATCAGA TCGAACTCAT TCCAAGTGAC 
CAGCTTGTCG CTGTGATCTC AGGCCGAAAT CGCCACGTCC GACTTTTTCC TATGTCAGCC 
TTGGATGGGC GAGAGACGGA TTTTTATAAA CTGGCAGAGA CTAAAGGGTG TCAGACCATA 
GCTGCTGGGA AAGTGCGCCA CGGAGCTCTG TCCTGCCTGT GTGTGGCCAT GAAGAGACAG 
GTCCTCTGCT ATGAACTGTT CCAAAGCAAG ACCAGGCACA GGAAGTTTAA GGAGATTCAA 
GTCCCATGTA ATGTCCAGTG GATGGCCATC TTCAGTGAGC ATCTCTGTGT GGGATTCCAG 
TCAGGATTTC TCAGATACCC CCTGAATGGA GAAGGAGGTC CATGCAATAT GCTCCATTCA 
AATGACCATA CACTGTCATT CATTTCACAC CAACCAATGG ACGCTCTCTG TGCAGTTGAG 
ATCTCCAACA AAGAGTACTT GCTGTGTTTT AACAGCATTG GGATATACAC AGACTGCCAG 
GGCCGAAGAT CCAGACAGCA GGAACTGATG TGGCCAGCAA ATCCTTCCTC CTGTTGTTAC 
AATGCTCCAT ATCTCTCCGT GTATAGTGAA AACGCAGTTG ATATCTTTGA TGTAAACTCC 
ATGGAATGGA TACAGACTCT TCCTCTCAAA AAGGTCCGAC CTTTAAACAC TGAAGGATCA 
TTAAATCTCT TAGGATTGGA GACCATTAGA TTAATATATT TCAAAAATAA GATGGCAGAA 
GGGGATGAAC TTGTGGTTCC TGAAACATCA GATAATAGTC GGAAACAAAT GGTTAGAAAT 
ATCAACAACA AACGGCGTTA CTCCTTCCGA GTCCCTGAAG AAGAGAGGAT GCAACAGAGG 
AGGGAGATGC TACGAGATCC AGAAATGAGA AATAAATTAA TTTCTAACCC AACTAATTTT 
AACCACATAG CACACATGGG TCCTGGAGAT GGAATACAGA TCCTAAAAGA CCTGCCCATG 
AACCCACGGC CTCAGGAAAG CCGGACAGTA TTCAGTGGCT CCGTCAGTAT TCCCTCCATC 
ACCAAGTCCC GCCCTGAGCC AGGCCGCTCC ATGAGTGCCA GCAGCGGCCT GTCTGCACGG 
TCGTCAGCCC AGAATGGCAG TGCACTAAAG AGGGAATTCT CTGGAGGAAG CTATAACACA 
AAGCGACAGC CCATGCCCTC TCCCTCGGAG GGCTCTTTGT CATCAGGAGG CATGGACCAA 
GGAAGTGACG CCCCTGCGAG GGACTATGAT GGAGAGGACT CTGATTCTCC AAGGCATTCC 
ACAGCTTCCA ACAGCTCTAA CCTGAGCAGC CCCCCAAGTC CCATTTCACC CCAAAAGACC 
AAGAGCCTCT CCCTGGAGAG CACAGACCGC GGGAGCTGGG ATCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_017320129.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175618.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X14, mRNA.

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CloneID	OMu77095
Clone ID Related Accession (Same CDS sequence)	XM_017320129.1 , XM_017320129.2
Accession Version	XM_017320129.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGCTG GAAGTTCACA CCGAAGCTCT AATTGCTGAA 
GCATCAAAGG ACAAGAAGCT CCGAGAGCAG AGCGAACATT ATTCTAAGCA ACTGGAAAAC 
GAGTTGGAGG GCCTGAAGCA AAAACAAATT AGTTACTCAC CAGGAATATG CAGCATAGAA 
CATCAGCAAG AGATAACTAA ACTGAAAACT GACTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAAATTT CTAAAAGAGA AGGAATTCAC GCAAGTGAGA TTAAAAACCT GAAGAAGGAG 
CTGCATGACT CAGAAGGCCA GCAGCTTGCT CTCAACAAAG AGATTCTGGT TTTAAAAGAC 
AAATTGGAAA AAACCAGGAG AGAAAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAACAACAAT ATGAACGGGA AAAAGTATTA TTAACTGAAG AGAATAAAAA GTTAACGAGT 
GAACTCGATA AGCTGACTTC ACTGTATGAG AGCTTAAGTT TGCGCAACCA GCACTTAGAA 
GAAGAAGTCA AAGATCTAGC AGATAAGAAG GAATCAGTTG CCCACTGGGA AGCCCAAATC 
ACAGAGATAA TTCAGTGGGT GAGCGATGAG AAGGATGCAC GAGGCTATCT TCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA GTTGGAAGCA TTAAGAAATT CCAGCTTGGG GACACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCAAAACTGG ACATGTCGGC TAGACTAGAG 
TTGCAGTCTG CTCTGGATGC AGAAATAAGA GCTAAGCAGG CCATCCAGGA AGAGCTGAAT 
AAAGTCAAAG CATCTAACAT CTTAACAGAA TGTAAACTGA AAGATTCAGA GAAGAAGAAC 
TTGGAACTGT TGTCCGAAAT CGAGCAGCTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ACCAATTTGA AGATTCCCTT TCTTCTTCCT CATCCTCACT GATTGATTTT 
TTGGATGACA TTGCAGACTG TGCTCCACTT CCAGCTCACA CACCAACCTT AAGGAAAAAG 
GGATGCCCGG CTTCAACTGG CTTTCCACCT AAGCGCAAGA CTCATCAATT TTTCGTGAAG 
TCTTTCACTG CCCCTACCAA GTGTCACCAG TGCACCTCCT TGATGGTCGG TTTGATAAGA 
CAGGGCTGTT CCTGTGAAGT CTGTGGGTTC TCATGTCATA TAACTTGTGT GAACAAAGCC 
CCGACGGTTT GCCCAGTTCC TCCTGAGCAA ACTAAAGGTC CACTGGGCAT AGACCCCCAG 
AAGGGAGTTG GGACGGCGTA TGAGGGTCAT GTCAGGATCC CTAAGCCGGC GGGAGTGAAG 
AAAGGGTGGC AGAGAGCACT GGCTGTGGTC TGCGACTTCA AACTGTTTCT TTATGATATC 
GCAGAGGGAA AAGCATCGCA GCCCACCTCC GTCATTAGTC AAGTGATTGA CATGAGAGAT 
GAAGAATTTT CTGTGAGTTC AGTCTTAGCT TCTGATGTTA TTCACGCAAG TCGAAAAGAT 
ATCCCCTGTA TATTCAGGGT CACAGCTTCC CAGCTCTCAG CACCTAGTAA CAAATGTTCC 
ATCCTGATGC TAGCAGACAG CGAGAATGAG AGGAGTAAGT GGGTGGGAGT GCTGAGTGAA 
CTCCACAAGA TTCTGAAGAA AAACAAGTTC AGAGACCGCT CAGTGTATGT CCCCAAGGAG 
GCGTACGACA GCACGCTACC CCTCATTAAG ACAACGCAGG CCGCTGCAAT CATAGATCAT 
GAACGGATAG CTTTGGGAAA TGAAGAAGGA TTATTTGTTG TGCATGTCAC CAAAGATGAG 
ATTGTTAGAG TTGGTGATAA TAAGAAAATC CATCAGATCG AACTCATTCC AAGTGACCAG 
CTTGTCGCTG TGATCTCAGG CCGAAATCGC CACGTCCGAC TTTTTCCTAT GTCAGCCTTG 
GATGGGCGAG AGACGGATTT TTATAAACTG GCAGAGACTA AAGGGTGTCA GACCATAGCT 
GCTGGGAAAG TGCGCCACGG AGCTCTGTCC TGCCTGTGTG TGGCCATGAA GAGACAGGTC 
CTCTGCTATG AACTGTTCCA AAGCAAGACC AGGCACAGGA AGTTTAAGGA GATTCAAGTC 
CCATGTAATG TCCAGTGGAT GGCCATCTTC AGTGAGCATC TCTGTGTGGG ATTCCAGTCA 
GGATTTCTCA GATACCCCCT GAATGGAGAA GGAGGTCCAT GCAATATGCT CCATTCAAAT 
GACCATACAC TGTCATTCAT TTCACACCAA CCAATGGACG CTCTCTGTGC AGTTGAGATC 
TCCAACAAAG AGTACTTGCT GTGTTTTAAC AGCATTGGGA TATACACAGA CTGCCAGGGC 
CGAAGATCCA GACAGCAGGA ACTGATGTGG CCAGCAAATC CTTCCTCCTG TTGTTACAAT 
GCTCCATATC TCTCCGTGTA TAGTGAAAAC GCAGTTGATA TCTTTGATGT AAACTCCATG 
GAATGGATAC AGACTCTTCC TCTCAAAAAG GTCCGACCTT TAAACACTGA AGGATCATTA 
AATCTCTTAG GATTGGAGAC CATTAGATTA ATATATTTCA AAAATAAGAT GGCAGAAGGG 
GATGAACTTG TGGTTCCTGA AACATCAGAT AATAGTCGGA AACAAATGGT TAGAAATATC 
AACAACAAAC GGCGTTACTC CTTCCGAGTC CCTGAAGAAG AGAGGATGCA ACAGAGGAGG 
GAGATGCTAC GAGATCCAGA AATGAGAAAT AAATTAATTT CTAACCCAAC TAATTTTAAC 
CACATAGCAC ACATGGGTCC TGGAGATGGA ATACAGATCC TAAAAGACCT GCCCATGCCA 
GGTTTCCCTT ATTCACCCCC TCATCACCAC TCCGGCCTGA TCTCCTCTCC GAGCAACTTT 
GAGCACATCT ATCACATGAC TGTCACTTCT GCTGAACAGT TTCTCTCTCC TGAGTCCATA 
AGCCCTGGGT CCCCCCCGTC CCTGCGCTCT GCCCCTGGCA CACCACACTC TGTGACTCTG 
AGGAACCCAC GGCCTCAGGA AAGCCGGACA GTATTCAGTG GCTCCGTCAG TATTCCCTCC 
ATCACCAAGT CCCGCCCTGA GCCAGGCCGC TCCATGAGTG CCAGCAGCGG CCTGTCTGCA 
CGGTCGTCAG CCCAGAATGG CAGTGCACTA AAGAGGGAAT TCTCTGGAGG AAGCTATAAC 
ACAAAGCGAC AGCCCATGCC CTCTCCCTCG GAGGGCTCTT TGTCATCAGG AGGCATGGAC 
CAAGGAAGTG ACGCCCCTGC GAGGGACTAT GATGGAGAGG ACTCTGATTC TCCAAGGCAT 
TCCACAGCTT CCAACAGCTC TAACCTGAGC AGCCCCCCAA GTCCCATTTC ACCCCAAAAG 
ACCAAGAGCC TCTCCCTGGA GAGCACAGAC CGCGGGAGCT GGGATCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175618.1
CDS	1142..6292
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKLEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSLSSSSSSLIDFLDDIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_017320129.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320129.1

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGCTG GAAGTTCACA CCGAAGCTCT AATTGCTGAA 
GCATCAAAGG ACAAGAAGCT CCGAGAGCAG AGCGAACATT ATTCTAAGCA ACTGGAAAAC 
GAGTTGGAGG GCCTGAAGCA AAAACAAATT AGTTACTCAC CAGGAATATG CAGCATAGAA 
CATCAGCAAG AGATAACTAA ACTGAAAACT GACTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAAATTT CTAAAAGAGA AGGAATTCAC GCAAGTGAGA TTAAAAACCT GAAGAAGGAG 
CTGCATGACT CAGAAGGCCA GCAGCTTGCT CTCAACAAAG AGATTCTGGT TTTAAAAGAC 
AAATTGGAAA AAACCAGGAG AGAAAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAACAACAAT ATGAACGGGA AAAAGTATTA TTAACTGAAG AGAATAAAAA GTTAACGAGT 
GAACTCGATA AGCTGACTTC ACTGTATGAG AGCTTAAGTT TGCGCAACCA GCACTTAGAA 
GAAGAAGTCA AAGATCTAGC AGATAAGAAG GAATCAGTTG CCCACTGGGA AGCCCAAATC 
ACAGAGATAA TTCAGTGGGT GAGCGATGAG AAGGATGCAC GAGGCTATCT TCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA GTTGGAAGCA TTAAGAAATT CCAGCTTGGG GACACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCAAAACTGG ACATGTCGGC TAGACTAGAG 
TTGCAGTCTG CTCTGGATGC AGAAATAAGA GCTAAGCAGG CCATCCAGGA AGAGCTGAAT 
AAAGTCAAAG CATCTAACAT CTTAACAGAA TGTAAACTGA AAGATTCAGA GAAGAAGAAC 
TTGGAACTGT TGTCCGAAAT CGAGCAGCTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ACCAATTTGA AGATTCCCTT TCTTCTTCCT CATCCTCACT GATTGATTTT 
TTGGATGACA TTGCAGACTG TGCTCCACTT CCAGCTCACA CACCAACCTT AAGGAAAAAG 
GGATGCCCGG CTTCAACTGG CTTTCCACCT AAGCGCAAGA CTCATCAATT TTTCGTGAAG 
TCTTTCACTG CCCCTACCAA GTGTCACCAG TGCACCTCCT TGATGGTCGG TTTGATAAGA 
CAGGGCTGTT CCTGTGAAGT CTGTGGGTTC TCATGTCATA TAACTTGTGT GAACAAAGCC 
CCGACGGTTT GCCCAGTTCC TCCTGAGCAA ACTAAAGGTC CACTGGGCAT AGACCCCCAG 
AAGGGAGTTG GGACGGCGTA TGAGGGTCAT GTCAGGATCC CTAAGCCGGC GGGAGTGAAG 
AAAGGGTGGC AGAGAGCACT GGCTGTGGTC TGCGACTTCA AACTGTTTCT TTATGATATC 
GCAGAGGGAA AAGCATCGCA GCCCACCTCC GTCATTAGTC AAGTGATTGA CATGAGAGAT 
GAAGAATTTT CTGTGAGTTC AGTCTTAGCT TCTGATGTTA TTCACGCAAG TCGAAAAGAT 
ATCCCCTGTA TATTCAGGGT CACAGCTTCC CAGCTCTCAG CACCTAGTAA CAAATGTTCC 
ATCCTGATGC TAGCAGACAG CGAGAATGAG AGGAGTAAGT GGGTGGGAGT GCTGAGTGAA 
CTCCACAAGA TTCTGAAGAA AAACAAGTTC AGAGACCGCT CAGTGTATGT CCCCAAGGAG 
GCGTACGACA GCACGCTACC CCTCATTAAG ACAACGCAGG CCGCTGCAAT CATAGATCAT 
GAACGGATAG CTTTGGGAAA TGAAGAAGGA TTATTTGTTG TGCATGTCAC CAAAGATGAG 
ATTGTTAGAG TTGGTGATAA TAAGAAAATC CATCAGATCG AACTCATTCC AAGTGACCAG 
CTTGTCGCTG TGATCTCAGG CCGAAATCGC CACGTCCGAC TTTTTCCTAT GTCAGCCTTG 
GATGGGCGAG AGACGGATTT TTATAAACTG GCAGAGACTA AAGGGTGTCA GACCATAGCT 
GCTGGGAAAG TGCGCCACGG AGCTCTGTCC TGCCTGTGTG TGGCCATGAA GAGACAGGTC 
CTCTGCTATG AACTGTTCCA AAGCAAGACC AGGCACAGGA AGTTTAAGGA GATTCAAGTC 
CCATGTAATG TCCAGTGGAT GGCCATCTTC AGTGAGCATC TCTGTGTGGG ATTCCAGTCA 
GGATTTCTCA GATACCCCCT GAATGGAGAA GGAGGTCCAT GCAATATGCT CCATTCAAAT 
GACCATACAC TGTCATTCAT TTCACACCAA CCAATGGACG CTCTCTGTGC AGTTGAGATC 
TCCAACAAAG AGTACTTGCT GTGTTTTAAC AGCATTGGGA TATACACAGA CTGCCAGGGC 
CGAAGATCCA GACAGCAGGA ACTGATGTGG CCAGCAAATC CTTCCTCCTG TTGTTACAAT 
GCTCCATATC TCTCCGTGTA TAGTGAAAAC GCAGTTGATA TCTTTGATGT AAACTCCATG 
GAATGGATAC AGACTCTTCC TCTCAAAAAG GTCCGACCTT TAAACACTGA AGGATCATTA 
AATCTCTTAG GATTGGAGAC CATTAGATTA ATATATTTCA AAAATAAGAT GGCAGAAGGG 
GATGAACTTG TGGTTCCTGA AACATCAGAT AATAGTCGGA AACAAATGGT TAGAAATATC 
AACAACAAAC GGCGTTACTC CTTCCGAGTC CCTGAAGAAG AGAGGATGCA ACAGAGGAGG 
GAGATGCTAC GAGATCCAGA AATGAGAAAT AAATTAATTT CTAACCCAAC TAATTTTAAC 
CACATAGCAC ACATGGGTCC TGGAGATGGA ATACAGATCC TAAAAGACCT GCCCATGCCA 
GGTTTCCCTT ATTCACCCCC TCATCACCAC TCCGGCCTGA TCTCCTCTCC GAGCAACTTT 
GAGCACATCT ATCACATGAC TGTCACTTCT GCTGAACAGT TTCTCTCTCC TGAGTCCATA 
AGCCCTGGGT CCCCCCCGTC CCTGCGCTCT GCCCCTGGCA CACCACACTC TGTGACTCTG 
AGGAACCCAC GGCCTCAGGA AAGCCGGACA GTATTCAGTG GCTCCGTCAG TATTCCCTCC 
ATCACCAAGT CCCGCCCTGA GCCAGGCCGC TCCATGAGTG CCAGCAGCGG CCTGTCTGCA 
CGGTCGTCAG CCCAGAATGG CAGTGCACTA AAGAGGGAAT TCTCTGGAGG AAGCTATAAC 
ACAAAGCGAC AGCCCATGCC CTCTCCCTCG GAGGGCTCTT TGTCATCAGG AGGCATGGAC 
CAAGGAAGTG ACGCCCCTGC GAGGGACTAT GATGGAGAGG ACTCTGATTC TCCAAGGCAT 
TCCACAGCTT CCAACAGCTC TAACCTGAGC AGCCCCCCAA GTCCCATTTC ACCCCAAAAG 
ACCAAGAGCC TCTCCCTGGA GAGCACAGAC CGCGGGAGCT GGGATCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_017320129.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175618.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X9, mRNA.

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CloneID	OMu77095
Clone ID Related Accession (Same CDS sequence)	XM_017320129.1 , XM_017320129.2
Accession Version	XM_017320129.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320129.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGCTG GAAGTTCACA CCGAAGCTCT AATTGCTGAA 
GCATCAAAGG ACAAGAAGCT CCGAGAGCAG AGCGAACATT ATTCTAAGCA ACTGGAAAAC 
GAGTTGGAGG GCCTGAAGCA AAAACAAATT AGTTACTCAC CAGGAATATG CAGCATAGAA 
CATCAGCAAG AGATAACTAA ACTGAAAACT GACTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAAATTT CTAAAAGAGA AGGAATTCAC GCAAGTGAGA TTAAAAACCT GAAGAAGGAG 
CTGCATGACT CAGAAGGCCA GCAGCTTGCT CTCAACAAAG AGATTCTGGT TTTAAAAGAC 
AAATTGGAAA AAACCAGGAG AGAAAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAACAACAAT ATGAACGGGA AAAAGTATTA TTAACTGAAG AGAATAAAAA GTTAACGAGT 
GAACTCGATA AGCTGACTTC ACTGTATGAG AGCTTAAGTT TGCGCAACCA GCACTTAGAA 
GAAGAAGTCA AAGATCTAGC AGATAAGAAG GAATCAGTTG CCCACTGGGA AGCCCAAATC 
ACAGAGATAA TTCAGTGGGT GAGCGATGAG AAGGATGCAC GAGGCTATCT TCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA GTTGGAAGCA TTAAGAAATT CCAGCTTGGG GACACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCAAAACTGG ACATGTCGGC TAGACTAGAG 
TTGCAGTCTG CTCTGGATGC AGAAATAAGA GCTAAGCAGG CCATCCAGGA AGAGCTGAAT 
AAAGTCAAAG CATCTAACAT CTTAACAGAA TGTAAACTGA AAGATTCAGA GAAGAAGAAC 
TTGGAACTGT TGTCCGAAAT CGAGCAGCTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ACCAATTTGA AGATTCCCTT TCTTCTTCCT CATCCTCACT GATTGATTTT 
TTGGATGACA TTGCAGACTG TGCTCCACTT CCAGCTCACA CACCAACCTT AAGGAAAAAG 
GGATGCCCGG CTTCAACTGG CTTTCCACCT AAGCGCAAGA CTCATCAATT TTTCGTGAAG 
TCTTTCACTG CCCCTACCAA GTGTCACCAG TGCACCTCCT TGATGGTCGG TTTGATAAGA 
CAGGGCTGTT CCTGTGAAGT CTGTGGGTTC TCATGTCATA TAACTTGTGT GAACAAAGCC 
CCGACGGTTT GCCCAGTTCC TCCTGAGCAA ACTAAAGGTC CACTGGGCAT AGACCCCCAG 
AAGGGAGTTG GGACGGCGTA TGAGGGTCAT GTCAGGATCC CTAAGCCGGC GGGAGTGAAG 
AAAGGGTGGC AGAGAGCACT GGCTGTGGTC TGCGACTTCA AACTGTTTCT TTATGATATC 
GCAGAGGGAA AAGCATCGCA GCCCACCTCC GTCATTAGTC AAGTGATTGA CATGAGAGAT 
GAAGAATTTT CTGTGAGTTC AGTCTTAGCT TCTGATGTTA TTCACGCAAG TCGAAAAGAT 
ATCCCCTGTA TATTCAGGGT CACAGCTTCC CAGCTCTCAG CACCTAGTAA CAAATGTTCC 
ATCCTGATGC TAGCAGACAG CGAGAATGAG AGGAGTAAGT GGGTGGGAGT GCTGAGTGAA 
CTCCACAAGA TTCTGAAGAA AAACAAGTTC AGAGACCGCT CAGTGTATGT CCCCAAGGAG 
GCGTACGACA GCACGCTACC CCTCATTAAG ACAACGCAGG CCGCTGCAAT CATAGATCAT 
GAACGGATAG CTTTGGGAAA TGAAGAAGGA TTATTTGTTG TGCATGTCAC CAAAGATGAG 
ATTGTTAGAG TTGGTGATAA TAAGAAAATC CATCAGATCG AACTCATTCC AAGTGACCAG 
CTTGTCGCTG TGATCTCAGG CCGAAATCGC CACGTCCGAC TTTTTCCTAT GTCAGCCTTG 
GATGGGCGAG AGACGGATTT TTATAAACTG GCAGAGACTA AAGGGTGTCA GACCATAGCT 
GCTGGGAAAG TGCGCCACGG AGCTCTGTCC TGCCTGTGTG TGGCCATGAA GAGACAGGTC 
CTCTGCTATG AACTGTTCCA AAGCAAGACC AGGCACAGGA AGTTTAAGGA GATTCAAGTC 
CCATGTAATG TCCAGTGGAT GGCCATCTTC AGTGAGCATC TCTGTGTGGG ATTCCAGTCA 
GGATTTCTCA GATACCCCCT GAATGGAGAA GGAGGTCCAT GCAATATGCT CCATTCAAAT 
GACCATACAC TGTCATTCAT TTCACACCAA CCAATGGACG CTCTCTGTGC AGTTGAGATC 
TCCAACAAAG AGTACTTGCT GTGTTTTAAC AGCATTGGGA TATACACAGA CTGCCAGGGC 
CGAAGATCCA GACAGCAGGA ACTGATGTGG CCAGCAAATC CTTCCTCCTG TTGTTACAAT 
GCTCCATATC TCTCCGTGTA TAGTGAAAAC GCAGTTGATA TCTTTGATGT AAACTCCATG 
GAATGGATAC AGACTCTTCC TCTCAAAAAG GTCCGACCTT TAAACACTGA AGGATCATTA 
AATCTCTTAG GATTGGAGAC CATTAGATTA ATATATTTCA AAAATAAGAT GGCAGAAGGG 
GATGAACTTG TGGTTCCTGA AACATCAGAT AATAGTCGGA AACAAATGGT TAGAAATATC 
AACAACAAAC GGCGTTACTC CTTCCGAGTC CCTGAAGAAG AGAGGATGCA ACAGAGGAGG 
GAGATGCTAC GAGATCCAGA AATGAGAAAT AAATTAATTT CTAACCCAAC TAATTTTAAC 
CACATAGCAC ACATGGGTCC TGGAGATGGA ATACAGATCC TAAAAGACCT GCCCATGCCA 
GGTTTCCCTT ATTCACCCCC TCATCACCAC TCCGGCCTGA TCTCCTCTCC GAGCAACTTT 
GAGCACATCT ATCACATGAC TGTCACTTCT GCTGAACAGT TTCTCTCTCC TGAGTCCATA 
AGCCCTGGGT CCCCCCCGTC CCTGCGCTCT GCCCCTGGCA CACCACACTC TGTGACTCTG 
AGGAACCCAC GGCCTCAGGA AAGCCGGACA GTATTCAGTG GCTCCGTCAG TATTCCCTCC 
ATCACCAAGT CCCGCCCTGA GCCAGGCCGC TCCATGAGTG CCAGCAGCGG CCTGTCTGCA 
CGGTCGTCAG CCCAGAATGG CAGTGCACTA AAGAGGGAAT TCTCTGGAGG AAGCTATAAC 
ACAAAGCGAC AGCCCATGCC CTCTCCCTCG GAGGGCTCTT TGTCATCAGG AGGCATGGAC 
CAAGGAAGTG ACGCCCCTGC GAGGGACTAT GATGGAGAGG ACTCTGATTC TCCAAGGCAT 
TCCACAGCTT CCAACAGCTC TAACCTGAGC AGCCCCCCAA GTCCCATTTC ACCCCAAAAG 
ACCAAGAGCC TCTCCCTGGA GAGCACAGAC CGCGGGAGCT GGGATCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175618.1
CDS	1032..6182
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKLEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSLSSSSSSLIDFLDDIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_017320129.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320129.2

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGCTG GAAGTTCACA CCGAAGCTCT AATTGCTGAA 
GCATCAAAGG ACAAGAAGCT CCGAGAGCAG AGCGAACATT ATTCTAAGCA ACTGGAAAAC 
GAGTTGGAGG GCCTGAAGCA AAAACAAATT AGTTACTCAC CAGGAATATG CAGCATAGAA 
CATCAGCAAG AGATAACTAA ACTGAAAACT GACTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAAATTT CTAAAAGAGA AGGAATTCAC GCAAGTGAGA TTAAAAACCT GAAGAAGGAG 
CTGCATGACT CAGAAGGCCA GCAGCTTGCT CTCAACAAAG AGATTCTGGT TTTAAAAGAC 
AAATTGGAAA AAACCAGGAG AGAAAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAACAACAAT ATGAACGGGA AAAAGTATTA TTAACTGAAG AGAATAAAAA GTTAACGAGT 
GAACTCGATA AGCTGACTTC ACTGTATGAG AGCTTAAGTT TGCGCAACCA GCACTTAGAA 
GAAGAAGTCA AAGATCTAGC AGATAAGAAG GAATCAGTTG CCCACTGGGA AGCCCAAATC 
ACAGAGATAA TTCAGTGGGT GAGCGATGAG AAGGATGCAC GAGGCTATCT TCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA GTTGGAAGCA TTAAGAAATT CCAGCTTGGG GACACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCAAAACTGG ACATGTCGGC TAGACTAGAG 
TTGCAGTCTG CTCTGGATGC AGAAATAAGA GCTAAGCAGG CCATCCAGGA AGAGCTGAAT 
AAAGTCAAAG CATCTAACAT CTTAACAGAA TGTAAACTGA AAGATTCAGA GAAGAAGAAC 
TTGGAACTGT TGTCCGAAAT CGAGCAGCTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ACCAATTTGA AGATTCCCTT TCTTCTTCCT CATCCTCACT GATTGATTTT 
TTGGATGACA TTGCAGACTG TGCTCCACTT CCAGCTCACA CACCAACCTT AAGGAAAAAG 
GGATGCCCGG CTTCAACTGG CTTTCCACCT AAGCGCAAGA CTCATCAATT TTTCGTGAAG 
TCTTTCACTG CCCCTACCAA GTGTCACCAG TGCACCTCCT TGATGGTCGG TTTGATAAGA 
CAGGGCTGTT CCTGTGAAGT CTGTGGGTTC TCATGTCATA TAACTTGTGT GAACAAAGCC 
CCGACGGTTT GCCCAGTTCC TCCTGAGCAA ACTAAAGGTC CACTGGGCAT AGACCCCCAG 
AAGGGAGTTG GGACGGCGTA TGAGGGTCAT GTCAGGATCC CTAAGCCGGC GGGAGTGAAG 
AAAGGGTGGC AGAGAGCACT GGCTGTGGTC TGCGACTTCA AACTGTTTCT TTATGATATC 
GCAGAGGGAA AAGCATCGCA GCCCACCTCC GTCATTAGTC AAGTGATTGA CATGAGAGAT 
GAAGAATTTT CTGTGAGTTC AGTCTTAGCT TCTGATGTTA TTCACGCAAG TCGAAAAGAT 
ATCCCCTGTA TATTCAGGGT CACAGCTTCC CAGCTCTCAG CACCTAGTAA CAAATGTTCC 
ATCCTGATGC TAGCAGACAG CGAGAATGAG AGGAGTAAGT GGGTGGGAGT GCTGAGTGAA 
CTCCACAAGA TTCTGAAGAA AAACAAGTTC AGAGACCGCT CAGTGTATGT CCCCAAGGAG 
GCGTACGACA GCACGCTACC CCTCATTAAG ACAACGCAGG CCGCTGCAAT CATAGATCAT 
GAACGGATAG CTTTGGGAAA TGAAGAAGGA TTATTTGTTG TGCATGTCAC CAAAGATGAG 
ATTGTTAGAG TTGGTGATAA TAAGAAAATC CATCAGATCG AACTCATTCC AAGTGACCAG 
CTTGTCGCTG TGATCTCAGG CCGAAATCGC CACGTCCGAC TTTTTCCTAT GTCAGCCTTG 
GATGGGCGAG AGACGGATTT TTATAAACTG GCAGAGACTA AAGGGTGTCA GACCATAGCT 
GCTGGGAAAG TGCGCCACGG AGCTCTGTCC TGCCTGTGTG TGGCCATGAA GAGACAGGTC 
CTCTGCTATG AACTGTTCCA AAGCAAGACC AGGCACAGGA AGTTTAAGGA GATTCAAGTC 
CCATGTAATG TCCAGTGGAT GGCCATCTTC AGTGAGCATC TCTGTGTGGG ATTCCAGTCA 
GGATTTCTCA GATACCCCCT GAATGGAGAA GGAGGTCCAT GCAATATGCT CCATTCAAAT 
GACCATACAC TGTCATTCAT TTCACACCAA CCAATGGACG CTCTCTGTGC AGTTGAGATC 
TCCAACAAAG AGTACTTGCT GTGTTTTAAC AGCATTGGGA TATACACAGA CTGCCAGGGC 
CGAAGATCCA GACAGCAGGA ACTGATGTGG CCAGCAAATC CTTCCTCCTG TTGTTACAAT 
GCTCCATATC TCTCCGTGTA TAGTGAAAAC GCAGTTGATA TCTTTGATGT AAACTCCATG 
GAATGGATAC AGACTCTTCC TCTCAAAAAG GTCCGACCTT TAAACACTGA AGGATCATTA 
AATCTCTTAG GATTGGAGAC CATTAGATTA ATATATTTCA AAAATAAGAT GGCAGAAGGG 
GATGAACTTG TGGTTCCTGA AACATCAGAT AATAGTCGGA AACAAATGGT TAGAAATATC 
AACAACAAAC GGCGTTACTC CTTCCGAGTC CCTGAAGAAG AGAGGATGCA ACAGAGGAGG 
GAGATGCTAC GAGATCCAGA AATGAGAAAT AAATTAATTT CTAACCCAAC TAATTTTAAC 
CACATAGCAC ACATGGGTCC TGGAGATGGA ATACAGATCC TAAAAGACCT GCCCATGCCA 
GGTTTCCCTT ATTCACCCCC TCATCACCAC TCCGGCCTGA TCTCCTCTCC GAGCAACTTT 
GAGCACATCT ATCACATGAC TGTCACTTCT GCTGAACAGT TTCTCTCTCC TGAGTCCATA 
AGCCCTGGGT CCCCCCCGTC CCTGCGCTCT GCCCCTGGCA CACCACACTC TGTGACTCTG 
AGGAACCCAC GGCCTCAGGA AAGCCGGACA GTATTCAGTG GCTCCGTCAG TATTCCCTCC 
ATCACCAAGT CCCGCCCTGA GCCAGGCCGC TCCATGAGTG CCAGCAGCGG CCTGTCTGCA 
CGGTCGTCAG CCCAGAATGG CAGTGCACTA AAGAGGGAAT TCTCTGGAGG AAGCTATAAC 
ACAAAGCGAC AGCCCATGCC CTCTCCCTCG GAGGGCTCTT TGTCATCAGG AGGCATGGAC 
CAAGGAAGTG ACGCCCCTGC GAGGGACTAT GATGGAGAGG ACTCTGATTC TCCAAGGCAT 
TCCACAGCTT CCAACAGCTC TAACCTGAGC AGCCCCCCAA GTCCCATTTC ACCCCAAAAG 
ACCAAGAGCC TCTCCCTGGA GAGCACAGAC CGCGGGAGCT GGGATCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_017320131.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175620.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X17, mRNA.

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CloneID	OMu77096
Clone ID Related Accession (Same CDS sequence)	XM_017320131.1 , NM_001359544.1
Accession Version	XM_017320131.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGCTG GAAGTTCACA CCGAAGCTCT AATTGCTGAA 
GCATCAAAGG ACAAGAAGCT CCGAGAGCAG AGCGAACATT ATTCTAAGCA ACTGGAAAAC 
GAGTTGGAGG GCCTGAAGCA AAAACAAATT AGTTACTCAC CAGGAATATG CAGCATAGAA 
CATCAGCAAG AGATAACTAA ACTGAAAACT GACTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAAATTT CTAAAAGAGA AGGAATTCAC GCAAGTGAGA TTAAAAACCT GAAGAAGGAG 
CTGCATGACT CAGAAGGCCA GCAGCTTGCT CTCAACAAAG AGATTCTGGT TTTAAAAGAC 
AAATTGGAAA AAACCAGGAG AGAAAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAACAACAAT ATGAACGGGA AAAAGTATTA TTAACTGAAG AGAATAAAAA GTTAACGAGT 
GAACTCGATA AGCTGACTTC ACTGTATGAG AGCTTAAGTT TGCGCAACCA GCACTTAGAA 
GAAGAAGTCA AAGATCTAGC AGATAAGAAG GAATCAGTTG CCCACTGGGA AGCCCAAATC 
ACAGAGATAA TTCAGTGGGT GAGCGATGAG AAGGATGCAC GAGGCTATCT TCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA GTTGGAAGCA TTAAGAAATT CCAGCTTGGG GACACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCAAAACTGG ACATGTCGGC TAGACTAGAG 
TTGCAGTCTG CTCTGGATGC AGAAATAAGA GCTAAGCAGG CCATCCAGGA AGAGCTGAAT 
AAAGTCAAAG CATCTAACAT CTTAACAGAA TGTAAACTGA AAGATTCAGA GAAGAAGAAC 
TTGGAACTGT TGTCCGAAAT CGAGCAGCTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ACCAATTTGA AATTGCAGAC TGTGCTCCAC TTCCAGCTCA CACACCAACC 
TTAAGGAAAA AGGGATGCCC GGCTTCAACT GGCTTTCCAC CTAAGCGCAA GACTCATCAA 
TTTTTCGTGA AGTCTTTCAC TGCCCCTACC AAGTGTCACC AGTGCACCTC CTTGATGGTC 
GGTTTGATAA GACAGGGCTG TTCCTGTGAA GTCTGTGGGT TCTCATGTCA TATAACTTGT 
GTGAACAAAG CCCCGACGGT TTGCCCAGTT CCTCCTGAGC AAACTAAAGG TCCACTGGGC 
ATAGACCCCC AGAAGGGAGT TGGGACGGCG TATGAGGGTC ATGTCAGGAT CCCTAAGCCG 
GCGGGAGTGA AGAAAGGGTG GCAGAGAGCA CTGGCTGTGG TCTGCGACTT CAAACTGTTT 
CTTTATGATA TCGCAGAGGG AAAAGCATCG CAGCCCACCT CCGTCATTAG TCAAGTGATT 
GACATGAGAG ATGAAGAATT TTCTGTGAGT TCAGTCTTAG CTTCTGATGT TATTCACGCA 
AGTCGAAAAG ATATCCCCTG TATATTCAGG GTCACAGCTT CCCAGCTCTC AGCACCTAGT 
AACAAATGTT CCATCCTGAT GCTAGCAGAC AGCGAGAATG AGAGGAGTAA GTGGGTGGGA 
GTGCTGAGTG AACTCCACAA GATTCTGAAG AAAAACAAGT TCAGAGACCG CTCAGTGTAT 
GTCCCCAAGG AGGCGTACGA CAGCACGCTA CCCCTCATTA AGACAACGCA GGCCGCTGCA 
ATCATAGATC ATGAACGGAT AGCTTTGGGA AATGAAGAAG GATTATTTGT TGTGCATGTC 
ACCAAAGATG AGATTGTTAG AGTTGGTGAT AATAAGAAAA TCCATCAGAT CGAACTCATT 
CCAAGTGACC AGCTTGTCGC TGTGATCTCA GGCCGAAATC GCCACGTCCG ACTTTTTCCT 
ATGTCAGCCT TGGATGGGCG AGAGACGGAT TTTTATAAAC TGGCAGAGAC TAAAGGGTGT 
CAGACCATAG CTGCTGGGAA AGTGCGCCAC GGAGCTCTGT CCTGCCTGTG TGTGGCCATG 
AAGAGACAGG TCCTCTGCTA TGAACTGTTC CAAAGCAAGA CCAGGCACAG GAAGTTTAAG 
GAGATTCAAG TCCCATGTAA TGTCCAGTGG ATGGCCATCT TCAGTGAGCA TCTCTGTGTG 
GGATTCCAGT CAGGATTTCT CAGATACCCC CTGAATGGAG AAGGAGGTCC ATGCAATATG 
CTCCATTCAA ATGACCATAC ACTGTCATTC ATTTCACACC AACCAATGGA CGCTCTCTGT 
GCAGTTGAGA TCTCCAACAA AGAGTACTTG CTGTGTTTTA ACAGCATTGG GATATACACA 
GACTGCCAGG GCCGAAGATC CAGACAGCAG GAACTGATGT GGCCAGCAAA TCCTTCCTCC 
TGTTGTTACA ATGCTCCATA TCTCTCCGTG TATAGTGAAA ACGCAGTTGA TATCTTTGAT 
GTAAACTCCA TGGAATGGAT ACAGACTCTT CCTCTCAAAA AGGTCCGACC TTTAAACACT 
GAAGGATCAT TAAATCTCTT AGGATTGGAG ACCATTAGAT TAATATATTT CAAAAATAAG 
ATGGCAGAAG GGGATGAACT TGTGGTTCCT GAAACATCAG ATAATAGTCG GAAACAAATG 
GTTAGAAATA TCAACAACAA ACGGCGTTAC TCCTTCCGAG TCCCTGAAGA AGAGAGGATG 
CAACAGAGGA GGGAGATGCT ACGAGATCCA GAAATGAGAA ATAAATTAAT TTCTAACCCA 
ACTAATTTTA ACCACATAGC ACACATGGGT CCTGGAGATG GAATACAGAT CCTAAAAGAC 
CTGCCCATGC CAGGTTTCCC TTATTCACCC CCTCATCACC ACTCCGGCCT GATCTCCTCT 
CCGAGCAACT TTGAGCACAT CTATCACATG ACTGTCACTT CTGCTGAACA GTTTCTCTCT 
CCTGAGTCCA TAAGCCCTGG GTCCCCCCCG TCCCTGCGCT CTGCCCCTGG CACACCACAC 
TCTGTGACTC TGAGGAACCC ACGGCCTCAG GAAAGCCGGA CAGTATTCAG TGGCTCCGTC 
AGTATTCCCT CCATCACCAA GTCCCGCCCT GAGCCAGGCC GCTCCATGAG TGCCAGCAGC 
GGCCTGTCTG CACGGTCGTC AGCCCAGAAT GGCAGTGCAC TAAAGAGGGA ATTCTCTGGA 
GGAAGCTATA ACACAAAGCG ACAGCCCATG CCCTCTCCCT CGGAGGGCTC TTTGTCATCA 
GGAGGCATGG ACCAAGGAAG TGACGCCCCT GCGAGGGACT ATGATGGAGA GGACTCTGAT 
TCTCCAAGGC ATTCCACAGC TTCCAACAGC TCTAACCTGA GCAGCCCCCC AAGTCCCATT 
TCACCCCAAA AGACCAAGAG CCTCTCCCTG GAGAGCACAG ACCGCGGGAG CTGGGATCCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175620.1
CDS	1142..6244
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKLEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_017320131.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320131.1

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGCTG GAAGTTCACA CCGAAGCTCT AATTGCTGAA 
GCATCAAAGG ACAAGAAGCT CCGAGAGCAG AGCGAACATT ATTCTAAGCA ACTGGAAAAC 
GAGTTGGAGG GCCTGAAGCA AAAACAAATT AGTTACTCAC CAGGAATATG CAGCATAGAA 
CATCAGCAAG AGATAACTAA ACTGAAAACT GACTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAAATTT CTAAAAGAGA AGGAATTCAC GCAAGTGAGA TTAAAAACCT GAAGAAGGAG 
CTGCATGACT CAGAAGGCCA GCAGCTTGCT CTCAACAAAG AGATTCTGGT TTTAAAAGAC 
AAATTGGAAA AAACCAGGAG AGAAAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAACAACAAT ATGAACGGGA AAAAGTATTA TTAACTGAAG AGAATAAAAA GTTAACGAGT 
GAACTCGATA AGCTGACTTC ACTGTATGAG AGCTTAAGTT TGCGCAACCA GCACTTAGAA 
GAAGAAGTCA AAGATCTAGC AGATAAGAAG GAATCAGTTG CCCACTGGGA AGCCCAAATC 
ACAGAGATAA TTCAGTGGGT GAGCGATGAG AAGGATGCAC GAGGCTATCT TCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA GTTGGAAGCA TTAAGAAATT CCAGCTTGGG GACACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCAAAACTGG ACATGTCGGC TAGACTAGAG 
TTGCAGTCTG CTCTGGATGC AGAAATAAGA GCTAAGCAGG CCATCCAGGA AGAGCTGAAT 
AAAGTCAAAG CATCTAACAT CTTAACAGAA TGTAAACTGA AAGATTCAGA GAAGAAGAAC 
TTGGAACTGT TGTCCGAAAT CGAGCAGCTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ACCAATTTGA AATTGCAGAC TGTGCTCCAC TTCCAGCTCA CACACCAACC 
TTAAGGAAAA AGGGATGCCC GGCTTCAACT GGCTTTCCAC CTAAGCGCAA GACTCATCAA 
TTTTTCGTGA AGTCTTTCAC TGCCCCTACC AAGTGTCACC AGTGCACCTC CTTGATGGTC 
GGTTTGATAA GACAGGGCTG TTCCTGTGAA GTCTGTGGGT TCTCATGTCA TATAACTTGT 
GTGAACAAAG CCCCGACGGT TTGCCCAGTT CCTCCTGAGC AAACTAAAGG TCCACTGGGC 
ATAGACCCCC AGAAGGGAGT TGGGACGGCG TATGAGGGTC ATGTCAGGAT CCCTAAGCCG 
GCGGGAGTGA AGAAAGGGTG GCAGAGAGCA CTGGCTGTGG TCTGCGACTT CAAACTGTTT 
CTTTATGATA TCGCAGAGGG AAAAGCATCG CAGCCCACCT CCGTCATTAG TCAAGTGATT 
GACATGAGAG ATGAAGAATT TTCTGTGAGT TCAGTCTTAG CTTCTGATGT TATTCACGCA 
AGTCGAAAAG ATATCCCCTG TATATTCAGG GTCACAGCTT CCCAGCTCTC AGCACCTAGT 
AACAAATGTT CCATCCTGAT GCTAGCAGAC AGCGAGAATG AGAGGAGTAA GTGGGTGGGA 
GTGCTGAGTG AACTCCACAA GATTCTGAAG AAAAACAAGT TCAGAGACCG CTCAGTGTAT 
GTCCCCAAGG AGGCGTACGA CAGCACGCTA CCCCTCATTA AGACAACGCA GGCCGCTGCA 
ATCATAGATC ATGAACGGAT AGCTTTGGGA AATGAAGAAG GATTATTTGT TGTGCATGTC 
ACCAAAGATG AGATTGTTAG AGTTGGTGAT AATAAGAAAA TCCATCAGAT CGAACTCATT 
CCAAGTGACC AGCTTGTCGC TGTGATCTCA GGCCGAAATC GCCACGTCCG ACTTTTTCCT 
ATGTCAGCCT TGGATGGGCG AGAGACGGAT TTTTATAAAC TGGCAGAGAC TAAAGGGTGT 
CAGACCATAG CTGCTGGGAA AGTGCGCCAC GGAGCTCTGT CCTGCCTGTG TGTGGCCATG 
AAGAGACAGG TCCTCTGCTA TGAACTGTTC CAAAGCAAGA CCAGGCACAG GAAGTTTAAG 
GAGATTCAAG TCCCATGTAA TGTCCAGTGG ATGGCCATCT TCAGTGAGCA TCTCTGTGTG 
GGATTCCAGT CAGGATTTCT CAGATACCCC CTGAATGGAG AAGGAGGTCC ATGCAATATG 
CTCCATTCAA ATGACCATAC ACTGTCATTC ATTTCACACC AACCAATGGA CGCTCTCTGT 
GCAGTTGAGA TCTCCAACAA AGAGTACTTG CTGTGTTTTA ACAGCATTGG GATATACACA 
GACTGCCAGG GCCGAAGATC CAGACAGCAG GAACTGATGT GGCCAGCAAA TCCTTCCTCC 
TGTTGTTACA ATGCTCCATA TCTCTCCGTG TATAGTGAAA ACGCAGTTGA TATCTTTGAT 
GTAAACTCCA TGGAATGGAT ACAGACTCTT CCTCTCAAAA AGGTCCGACC TTTAAACACT 
GAAGGATCAT TAAATCTCTT AGGATTGGAG ACCATTAGAT TAATATATTT CAAAAATAAG 
ATGGCAGAAG GGGATGAACT TGTGGTTCCT GAAACATCAG ATAATAGTCG GAAACAAATG 
GTTAGAAATA TCAACAACAA ACGGCGTTAC TCCTTCCGAG TCCCTGAAGA AGAGAGGATG 
CAACAGAGGA GGGAGATGCT ACGAGATCCA GAAATGAGAA ATAAATTAAT TTCTAACCCA 
ACTAATTTTA ACCACATAGC ACACATGGGT CCTGGAGATG GAATACAGAT CCTAAAAGAC 
CTGCCCATGC CAGGTTTCCC TTATTCACCC CCTCATCACC ACTCCGGCCT GATCTCCTCT 
CCGAGCAACT TTGAGCACAT CTATCACATG ACTGTCACTT CTGCTGAACA GTTTCTCTCT 
CCTGAGTCCA TAAGCCCTGG GTCCCCCCCG TCCCTGCGCT CTGCCCCTGG CACACCACAC 
TCTGTGACTC TGAGGAACCC ACGGCCTCAG GAAAGCCGGA CAGTATTCAG TGGCTCCGTC 
AGTATTCCCT CCATCACCAA GTCCCGCCCT GAGCCAGGCC GCTCCATGAG TGCCAGCAGC 
GGCCTGTCTG CACGGTCGTC AGCCCAGAAT GGCAGTGCAC TAAAGAGGGA ATTCTCTGGA 
GGAAGCTATA ACACAAAGCG ACAGCCCATG CCCTCTCCCT CGGAGGGCTC TTTGTCATCA 
GGAGGCATGG ACCAAGGAAG TGACGCCCCT GCGAGGGACT ATGATGGAGA GGACTCTGAT 
TCTCCAAGGC ATTCCACAGC TTCCAACAGC TCTAACCTGA GCAGCCCCCC AAGTCCCATT 
TCACCCCAAA AGACCAAGAG CCTCTCCCTG GAGAGCACAG ACCGCGGGAG CTGGGATCCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_017320132.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175621.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X19, mRNA.

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CloneID	OMu77097
Clone ID Related Accession (Same CDS sequence)	XM_017320132.1 , NM_001359546.1
Accession Version	XM_017320132.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATTAC ATAGAGAAGA CTTTGAAATA TTAAAGGTGA TTGGTCGAGG AGCTTTTGGG 
GAGGTTGCTG TAGTTAAACT AAAAAATGCA GATAAAGTAT TCGCCATGAA AATTCTGAAC 
AAGTGGGAAA TGCTGAAAAG AGCTGAGACA GCGTGCTTTC GTGAAGAGAG GGATGTGTTG 
GTAAATGGAG ACAGTAAGTG GATAACCACT CTGCACTATG CTTTCCAGGA TGACAATAAC 
TTATACCTGG TTATGGATTA TTATGTTGGT GGGGATTTAC TTACTCTGCT CAGCAAATTT 
GAAGATAGAT TGCCTGAAGA GATGGCTCGG TTTTACTTGG CTGAGATGGT GATAGCCATT 
GACTCAGTTC ACCAGCTCCA CTATGTTCAC AGAGACATCA AACCTGACAA TATATTGATG 
GATATGAATG GACATATTCG TTTAGCAGAT TTTGGTTCTT GTCTGAAGCT GATGGAAGAT 
GGAACGGTCC AGTCCTCGGT GGCAGTTGGA ACTCCAGATT ATATTTCTCC GGAAATCCTT 
CAGGCTATGG AAGACGGAAA AGGCAGATAT GGACCAGAGT GTGACTGGTG GTCCTTGGGG 
GTCTGTATGT ATGAGATGCT TTACGGAGAA ACACCATTCT ATGCAGAGTC TCTGGTGGAG 
ACATATGGAA AAATTATGAA TCACAAAGAG CGGTTTCAGT TTCCAGCCCA AGTGACAGAC 
GTGTCCGAAA ATGCTAAAGA TCTTATTCGA AGACTCATTT GTAGTAGAGA ACATCGACTT 
GGCCAAAATG GAATAGAAGA CTTTAAGAAG CATCCATTCT TCAGTGGAAT TGATTGGGAT 
AACATTCGGA ATTGTGAAGC ACCTTACATC CCAGAAGTTA GTAGCCCGAC AGATACGTCA 
AATTTTGATG TGGATGACGA CTGTTTAAAA AATTCTGAAA CAATGCCCCC ACCAACACAT 
ACTGCATTTT CTGGCCATCA TCTACCATTT GTTGGTTTTA CATATACTAG TAGCTGTGTT 
CTTTCTGATC GGAGCTGTTT AAGAGTCACA GCAGGCCCCA CCTCATTGGA CCTTGATGTC 
AGTGTCCAGA GGACTCTAGA CAATAACTTA GCAACTGAGG CTTATGAAAG GAGAATTAAG 
CGGCTTGAGC AGGAAAAGCT TGAACTGACT AGAAAACTTC AAGAGTCAAC ACAGACTGTT 
CAAGCTCTGC AATATTCAAC TGTTGATGGT CCACTAACGG CAAGCAAAGA TTTAGAAATT 
AAAAGTCTGA AAGAAGAGAT TGAAAAACTA AGGAAACAAG TAGCAGAAGT GAATCATTTG 
GAACAACAAC TTGAAGAAGC TAATTCTGTG AGACGAGAAC TAGATGATGC TTTCAGGCAA 
ATCAAAGCTT CTGAAAAACA AATCAAAACT TTGCAGCAGG AAAGAGAAGA ACTAAATAAG 
GAACTAGTCC AGGCTAGTGA GCGATTAAAA AACCAATCCA AAGAGCTGAA AGATGCACAC 
TGTCAGAGGA AACTGGCCAT GCAGGAATTC ATGGAGATCA ATGAGCGACT AACAGAATTG 
CACACCCAAA AGCAGAAACT TGCTCGCCAT GTCCGAGATA AGGAAGAAGA GGTGGACCTG 
GTGATGCAAA AAGCTGAGAG CTTAAGGCAG GAGCTGCGCA GAGCAGAAAG AGCCAAGAAA 
GAGCTGGAAG TTCACACCGA AGCTCTAATT GCTGAAGCAT CAAAGGACAA GAAGCTCCGA 
GAGCAGAGCG AACATTATTC TAAGCAACTG GAAAACGAGT TGGAGGGCCT GAAGCAAAAA 
CAAATTAGTT ACTCACCAGG AATATGCAGC ATAGAACATC AGCAAGAGAT AACTAAACTG 
AAAACTGACT TGGAAAAGAA AAGTATCTTT TATGAAGAAG AAATTTCTAA AAGAGAAGGA 
ATTCACGCAA GTGAGATTAA AAACCTGAAG AAGGAGCTGC ATGACTCAGA AGGCCAGCAG 
CTTGCTCTCA ACAAAGAGAT TCTGGTTTTA AAAGACAAAT TGGAAAAAAC CAGGAGAGAA 
AGTCAAAGTG AAAGGGAAGA ATTTGAAAAT GAGTTCAAAC AACAATATGA ACGGGAAAAA 
GTATTATTAA CTGAAGAGAA TAAAAAGTTA ACGAGTGAAC TCGATAAGCT GACTTCACTG 
TATGAGAGCT TAAGTTTGCG CAACCAGCAC TTAGAAGAAG AAGTCAAAGA TCTAGCAGAT 
AAGAAGGAAT CAGTTGCCCA CTGGGAAGCC CAAATCACAG AGATAATTCA GTGGGTGAGC 
GATGAGAAGG ATGCACGAGG CTATCTTCAG GCCTTAGCTT CTAAAATGAC TGAAGAGTTG 
GAAGCATTAA GAAATTCCAG CTTGGGGACA CGAGCAACAG ATATGCCCTG GAAAATGCGT 
CGTTTTGCAA AACTGGACAT GTCGGCTAGA CTAGAGTTGC AGTCTGCTCT GGATGCAGAA 
ATAAGAGCTA AGCAGGCCAT CCAGGAAGAG CTGAATAAAG TCAAAGCATC TAACATCTTA 
ACAGAATGTA AACTGAAAGA TTCAGAGAAG AAGAACTTGG AACTGTTGTC CGAAATCGAG 
CAGCTGATAA AGGACACTGA AGAGCTTAGA TCTGAAAAGG GTATAGAGCA CCAAGACTCA 
CAGCATTCTT TCTTGGCATT TTTGAATACG CCTACCGATG CTCTGGACCA ATTTGAACGC 
TCTCCGTCCT GTACTCCAGC TAGCAAAGGC AGACGTATTG CAGACTGTGC TCCACTTCCA 
GCTCACACAC CAACCTTAAG GAAAAAGGGA TGCCCGGCTT CAACTGGCTT TCCACCTAAG 
CGCAAGACTC ATCAATTTTT CGTGAAGTCT TTCACTGCCC CTACCAAGTG TCACCAGTGC 
ACCTCCTTGA TGGTCGGTTT GATAAGACAG GGCTGTTCCT GTGAAGTCTG TGGGTTCTCA 
TGTCATATAA CTTGTGTGAA CAAAGCCCCG ACGGTTTGCC CAGTTCCTCC TGAGCAAACT 
AAAGGTCCAC TGGGCATAGA CCCCCAGAAG GGAGTTGGGA CGGCGTATGA GGGTCATGTC 
AGGATCCCTA AGCCGGCGGG AGTGAAGAAA GGGTGGCAGA GAGCACTGGC TGTGGTCTGC 
GACTTCAAAC TGTTTCTTTA TGATATCGCA GAGGGAAAAG CATCGCAGCC CACCTCCGTC 
ATTAGTCAAG TGATTGACAT GAGAGATGAA GAATTTTCTG TGAGTTCAGT CTTAGCTTCT 
GATGTTATTC ACGCAAGTCG AAAAGATATC CCCTGTATAT TCAGGGTCAC AGCTTCCCAG 
CTCTCAGCAC CTAGTAACAA ATGTTCCATC CTGATGCTAG CAGACAGCGA GAATGAGAGG 
AGTAAGTGGG TGGGAGTGCT GAGTGAACTC CACAAGATTC TGAAGAAAAA CAAGTTCAGA 
GACCGCTCAG TGTATGTCCC CAAGGAGGCG TACGACAGCA CGCTACCCCT CATTAAGACA 
ACGCAGGCCG CTGCAATCAT AGATCATGAA CGGATAGCTT TGGGAAATGA AGAAGGATTA 
TTTGTTGTGC ATGTCACCAA AGATGAGATT GTTAGAGTTG GTGATAATAA GAAAATCCAT 
CAGATCGAAC TCATTCCAAG TGACCAGCTT GTCGCTGTGA TCTCAGGCCG AAATCGCCAC 
GTCCGACTTT TTCCTATGTC AGCCTTGGAT GGGCGAGAGA CGGATTTTTA TAAACTGGCA 
GAGACTAAAG GGTGTCAGAC CATAGCTGCT GGGAAAGTGC GCCACGGAGC TCTGTCCTGC 
CTGTGTGTGG CCATGAAGAG ACAGGTCCTC TGCTATGAAC TGTTCCAAAG CAAGACCAGG 
CACAGGAAGT TTAAGGAGAT TCAAGTCCCA TGTAATGTCC AGTGGATGGC CATCTTCAGT 
GAGCATCTCT GTGTGGGATT CCAGTCAGGA TTTCTCAGAT ACCCCCTGAA TGGAGAAGGA 
GGTCCATGCA ATATGCTCCA TTCAAATGAC CATACACTGT CATTCATTTC ACACCAACCA 
ATGGACGCTC TCTGTGCAGT TGAGATCTCC AACAAAGAGT ACTTGCTGTG TTTTAACAGC 
ATTGGGATAT ACACAGACTG CCAGGGCCGA AGATCCAGAC AGCAGGAACT GATGTGGCCA 
GCAAATCCTT CCTCCTGTTG TTACAATGCT CCATATCTCT CCGTGTATAG TGAAAACGCA 
GTTGATATCT TTGATGTAAA CTCCATGGAA TGGATACAGA CTCTTCCTCT CAAAAAGGTC 
CGACCTTTAA ACACTGAAGG ATCATTAAAT CTCTTAGGAT TGGAGACCAT TAGATTAATA 
TATTTCAAAA ATAAGATGGC AGAAGGGGAT GAACTTGTGG TTCCTGAAAC ATCAGATAAT 
AGTCGGAAAC AAATGGTTAG AAATATCAAC AACAAACGGC GTTACTCCTT CCGAGTCCCT 
GAAGAAGAGA GGATGCAACA GAGGAGGGAG ATGCTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ACCCAACTAA TTTTAACCAC ATAGCACACA TGGGTCCTGG AGATGGAATA 
CAGATCCTAA AAGACCTGCC CATGAACCCA CGGCCTCAGG AAAGCCGGAC AGTATTCAGT 
GGCTCCGTCA GTATTCCCTC CATCACCAAG TCCCGCCCTG AGCCAGGCCG CTCCATGAGT 
GCCAGCAGCG GCCTGTCTGC ACGGTCGTCA GCCCAGAATG GCAGTGCACT AAAGAGGGAA 
TTCTCTGGAG GAAGCTATAA CACAAAGCGA CAGCCCATGC CCTCTCCCTC GGAGGGCTCT 
TTGTCATCAG GAGGCATGGA CCAAGGAAGT GACGCCCCTG CGAGGGACTA TGATGGAGAG 
GACTCTGATT CTCCAAGGCA TTCCACAGCT TCCAACAGCT CTAACCTGAG CAGCCCCCCA 
AGTCCCATTT CACCCCAAAA GACCAAGAGC CTCTCCCTGG AGAGCACAGA CCGCGGGAGC 
TGGGATCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175621.1
CDS	1421..6412
Translation	MRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_017320132.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320132.1

ORF Insert Sequence:

ATGCGATTAC ATAGAGAAGA CTTTGAAATA TTAAAGGTGA TTGGTCGAGG AGCTTTTGGG 
GAGGTTGCTG TAGTTAAACT AAAAAATGCA GATAAAGTAT TCGCCATGAA AATTCTGAAC 
AAGTGGGAAA TGCTGAAAAG AGCTGAGACA GCGTGCTTTC GTGAAGAGAG GGATGTGTTG 
GTAAATGGAG ACAGTAAGTG GATAACCACT CTGCACTATG CTTTCCAGGA TGACAATAAC 
TTATACCTGG TTATGGATTA TTATGTTGGT GGGGATTTAC TTACTCTGCT CAGCAAATTT 
GAAGATAGAT TGCCTGAAGA GATGGCTCGG TTTTACTTGG CTGAGATGGT GATAGCCATT 
GACTCAGTTC ACCAGCTCCA CTATGTTCAC AGAGACATCA AACCTGACAA TATATTGATG 
GATATGAATG GACATATTCG TTTAGCAGAT TTTGGTTCTT GTCTGAAGCT GATGGAAGAT 
GGAACGGTCC AGTCCTCGGT GGCAGTTGGA ACTCCAGATT ATATTTCTCC GGAAATCCTT 
CAGGCTATGG AAGACGGAAA AGGCAGATAT GGACCAGAGT GTGACTGGTG GTCCTTGGGG 
GTCTGTATGT ATGAGATGCT TTACGGAGAA ACACCATTCT ATGCAGAGTC TCTGGTGGAG 
ACATATGGAA AAATTATGAA TCACAAAGAG CGGTTTCAGT TTCCAGCCCA AGTGACAGAC 
GTGTCCGAAA ATGCTAAAGA TCTTATTCGA AGACTCATTT GTAGTAGAGA ACATCGACTT 
GGCCAAAATG GAATAGAAGA CTTTAAGAAG CATCCATTCT TCAGTGGAAT TGATTGGGAT 
AACATTCGGA ATTGTGAAGC ACCTTACATC CCAGAAGTTA GTAGCCCGAC AGATACGTCA 
AATTTTGATG TGGATGACGA CTGTTTAAAA AATTCTGAAA CAATGCCCCC ACCAACACAT 
ACTGCATTTT CTGGCCATCA TCTACCATTT GTTGGTTTTA CATATACTAG TAGCTGTGTT 
CTTTCTGATC GGAGCTGTTT AAGAGTCACA GCAGGCCCCA CCTCATTGGA CCTTGATGTC 
AGTGTCCAGA GGACTCTAGA CAATAACTTA GCAACTGAGG CTTATGAAAG GAGAATTAAG 
CGGCTTGAGC AGGAAAAGCT TGAACTGACT AGAAAACTTC AAGAGTCAAC ACAGACTGTT 
CAAGCTCTGC AATATTCAAC TGTTGATGGT CCACTAACGG CAAGCAAAGA TTTAGAAATT 
AAAAGTCTGA AAGAAGAGAT TGAAAAACTA AGGAAACAAG TAGCAGAAGT GAATCATTTG 
GAACAACAAC TTGAAGAAGC TAATTCTGTG AGACGAGAAC TAGATGATGC TTTCAGGCAA 
ATCAAAGCTT CTGAAAAACA AATCAAAACT TTGCAGCAGG AAAGAGAAGA ACTAAATAAG 
GAACTAGTCC AGGCTAGTGA GCGATTAAAA AACCAATCCA AAGAGCTGAA AGATGCACAC 
TGTCAGAGGA AACTGGCCAT GCAGGAATTC ATGGAGATCA ATGAGCGACT AACAGAATTG 
CACACCCAAA AGCAGAAACT TGCTCGCCAT GTCCGAGATA AGGAAGAAGA GGTGGACCTG 
GTGATGCAAA AAGCTGAGAG CTTAAGGCAG GAGCTGCGCA GAGCAGAAAG AGCCAAGAAA 
GAGCTGGAAG TTCACACCGA AGCTCTAATT GCTGAAGCAT CAAAGGACAA GAAGCTCCGA 
GAGCAGAGCG AACATTATTC TAAGCAACTG GAAAACGAGT TGGAGGGCCT GAAGCAAAAA 
CAAATTAGTT ACTCACCAGG AATATGCAGC ATAGAACATC AGCAAGAGAT AACTAAACTG 
AAAACTGACT TGGAAAAGAA AAGTATCTTT TATGAAGAAG AAATTTCTAA AAGAGAAGGA 
ATTCACGCAA GTGAGATTAA AAACCTGAAG AAGGAGCTGC ATGACTCAGA AGGCCAGCAG 
CTTGCTCTCA ACAAAGAGAT TCTGGTTTTA AAAGACAAAT TGGAAAAAAC CAGGAGAGAA 
AGTCAAAGTG AAAGGGAAGA ATTTGAAAAT GAGTTCAAAC AACAATATGA ACGGGAAAAA 
GTATTATTAA CTGAAGAGAA TAAAAAGTTA ACGAGTGAAC TCGATAAGCT GACTTCACTG 
TATGAGAGCT TAAGTTTGCG CAACCAGCAC TTAGAAGAAG AAGTCAAAGA TCTAGCAGAT 
AAGAAGGAAT CAGTTGCCCA CTGGGAAGCC CAAATCACAG AGATAATTCA GTGGGTGAGC 
GATGAGAAGG ATGCACGAGG CTATCTTCAG GCCTTAGCTT CTAAAATGAC TGAAGAGTTG 
GAAGCATTAA GAAATTCCAG CTTGGGGACA CGAGCAACAG ATATGCCCTG GAAAATGCGT 
CGTTTTGCAA AACTGGACAT GTCGGCTAGA CTAGAGTTGC AGTCTGCTCT GGATGCAGAA 
ATAAGAGCTA AGCAGGCCAT CCAGGAAGAG CTGAATAAAG TCAAAGCATC TAACATCTTA 
ACAGAATGTA AACTGAAAGA TTCAGAGAAG AAGAACTTGG AACTGTTGTC CGAAATCGAG 
CAGCTGATAA AGGACACTGA AGAGCTTAGA TCTGAAAAGG GTATAGAGCA CCAAGACTCA 
CAGCATTCTT TCTTGGCATT TTTGAATACG CCTACCGATG CTCTGGACCA ATTTGAACGC 
TCTCCGTCCT GTACTCCAGC TAGCAAAGGC AGACGTATTG CAGACTGTGC TCCACTTCCA 
GCTCACACAC CAACCTTAAG GAAAAAGGGA TGCCCGGCTT CAACTGGCTT TCCACCTAAG 
CGCAAGACTC ATCAATTTTT CGTGAAGTCT TTCACTGCCC CTACCAAGTG TCACCAGTGC 
ACCTCCTTGA TGGTCGGTTT GATAAGACAG GGCTGTTCCT GTGAAGTCTG TGGGTTCTCA 
TGTCATATAA CTTGTGTGAA CAAAGCCCCG ACGGTTTGCC CAGTTCCTCC TGAGCAAACT 
AAAGGTCCAC TGGGCATAGA CCCCCAGAAG GGAGTTGGGA CGGCGTATGA GGGTCATGTC 
AGGATCCCTA AGCCGGCGGG AGTGAAGAAA GGGTGGCAGA GAGCACTGGC TGTGGTCTGC 
GACTTCAAAC TGTTTCTTTA TGATATCGCA GAGGGAAAAG CATCGCAGCC CACCTCCGTC 
ATTAGTCAAG TGATTGACAT GAGAGATGAA GAATTTTCTG TGAGTTCAGT CTTAGCTTCT 
GATGTTATTC ACGCAAGTCG AAAAGATATC CCCTGTATAT TCAGGGTCAC AGCTTCCCAG 
CTCTCAGCAC CTAGTAACAA ATGTTCCATC CTGATGCTAG CAGACAGCGA GAATGAGAGG 
AGTAAGTGGG TGGGAGTGCT GAGTGAACTC CACAAGATTC TGAAGAAAAA CAAGTTCAGA 
GACCGCTCAG TGTATGTCCC CAAGGAGGCG TACGACAGCA CGCTACCCCT CATTAAGACA 
ACGCAGGCCG CTGCAATCAT AGATCATGAA CGGATAGCTT TGGGAAATGA AGAAGGATTA 
TTTGTTGTGC ATGTCACCAA AGATGAGATT GTTAGAGTTG GTGATAATAA GAAAATCCAT 
CAGATCGAAC TCATTCCAAG TGACCAGCTT GTCGCTGTGA TCTCAGGCCG AAATCGCCAC 
GTCCGACTTT TTCCTATGTC AGCCTTGGAT GGGCGAGAGA CGGATTTTTA TAAACTGGCA 
GAGACTAAAG GGTGTCAGAC CATAGCTGCT GGGAAAGTGC GCCACGGAGC TCTGTCCTGC 
CTGTGTGTGG CCATGAAGAG ACAGGTCCTC TGCTATGAAC TGTTCCAAAG CAAGACCAGG 
CACAGGAAGT TTAAGGAGAT TCAAGTCCCA TGTAATGTCC AGTGGATGGC CATCTTCAGT 
GAGCATCTCT GTGTGGGATT CCAGTCAGGA TTTCTCAGAT ACCCCCTGAA TGGAGAAGGA 
GGTCCATGCA ATATGCTCCA TTCAAATGAC CATACACTGT CATTCATTTC ACACCAACCA 
ATGGACGCTC TCTGTGCAGT TGAGATCTCC AACAAAGAGT ACTTGCTGTG TTTTAACAGC 
ATTGGGATAT ACACAGACTG CCAGGGCCGA AGATCCAGAC AGCAGGAACT GATGTGGCCA 
GCAAATCCTT CCTCCTGTTG TTACAATGCT CCATATCTCT CCGTGTATAG TGAAAACGCA 
GTTGATATCT TTGATGTAAA CTCCATGGAA TGGATACAGA CTCTTCCTCT CAAAAAGGTC 
CGACCTTTAA ACACTGAAGG ATCATTAAAT CTCTTAGGAT TGGAGACCAT TAGATTAATA 
TATTTCAAAA ATAAGATGGC AGAAGGGGAT GAACTTGTGG TTCCTGAAAC ATCAGATAAT 
AGTCGGAAAC AAATGGTTAG AAATATCAAC AACAAACGGC GTTACTCCTT CCGAGTCCCT 
GAAGAAGAGA GGATGCAACA GAGGAGGGAG ATGCTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ACCCAACTAA TTTTAACCAC ATAGCACACA TGGGTCCTGG AGATGGAATA 
CAGATCCTAA AAGACCTGCC CATGAACCCA CGGCCTCAGG AAAGCCGGAC AGTATTCAGT 
GGCTCCGTCA GTATTCCCTC CATCACCAAG TCCCGCCCTG AGCCAGGCCG CTCCATGAGT 
GCCAGCAGCG GCCTGTCTGC ACGGTCGTCA GCCCAGAATG GCAGTGCACT AAAGAGGGAA 
TTCTCTGGAG GAAGCTATAA CACAAAGCGA CAGCCCATGC CCTCTCCCTC GGAGGGCTCT 
TTGTCATCAG GAGGCATGGA CCAAGGAAGT GACGCCCCTG CGAGGGACTA TGATGGAGAG 
GACTCTGATT CTCCAAGGCA TTCCACAGCT TCCAACAGCT CTAACCTGAG CAGCCCCCCA 
AGTCCCATTT CACCCCAAAA GACCAAGAGC CTCTCCCTGG AGAGCACAGA CCGCGGGAGC 
TGGGATCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_017320134.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175623.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X20, mRNA.

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CloneID	OMu77098
Clone ID Related Accession (Same CDS sequence)	XM_017320134.1 , NM_001359547.1
Accession Version	XM_017320134.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGATG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGACTAACAG AATTGCACAC CCAAAAGCAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGCT GAGAGCTTAA GGCAGGAGCT GCGCAGAGCA 
GAAAGAGCCA AGAAAGAGCT GGAAGTTCAC ACCGAAGCTC TAATTGCTGA AGCATCAAAG 
GACAAGAAGC TCCGAGAGCA GAGCGAACAT TATTCTAAGC AACTGGAAAA CGAGTTGGAG 
GGCCTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAATAT GCAGCATAGA ACATCAGCAA 
GAGATAACTA AACTGAAAAC TGACTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAAATT 
TCTAAAAGAG AAGGAATTCA CGCAAGTGAG ATTAAAAACC TGAAGAAGGA GCTGCATGAC 
TCAGAAGGCC AGCAGCTTGC TCTCAACAAA GAGATTCTGG TTTTAAAAGA CAAATTGGAA 
AAAACCAGGA GAGAAAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAACAACAA 
TATGAACGGG AAAAAGTATT ATTAACTGAA GAGAATAAAA AGTTAACGAG TGAACTCGAT 
AAGCTGACTT CACTGTATGA GAGCTTAAGT TTGCGCAACC AGCACTTAGA AGAAGAAGTC 
AAAGATCTAG CAGATAAGAA GGAATCAGTT GCCCACTGGG AAGCCCAAAT CACAGAGATA 
ATTCAGTGGG TGAGCGATGA GAAGGATGCA CGAGGCTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AGTTGGAAGC ATTAAGAAAT TCCAGCTTGG GGACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCAAAACTG GACATGTCGG CTAGACTAGA GTTGCAGTCT 
GCTCTGGATG CAGAAATAAG AGCTAAGCAG GCCATCCAGG AAGAGCTGAA TAAAGTCAAA 
GCATCTAACA TCTTAACAGA ATGTAAACTG AAAGATTCAG AGAAGAAGAA CTTGGAACTG 
TTGTCCGAAA TCGAGCAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GACCAATTTG AAGATTCCCT TTCTTCTTCC TCATCCTCAC TGATTGATTT TTTGGATGAC 
CGCTCTCCGT CCTGTACTCC AGCTAGCAAA GGCAGACGTA TTGCAGACTG TGCTCCACTT 
CCAGCTCACA CACCAACCTT AAGGAAAAAG GGATGCCCGG CTTCAACTGG CTTTCCACCT 
AAGCGCAAGA CTCATCAATT TTTCGTGAAG TCTTTCACTG CCCCTACCAA GTGTCACCAG 
TGCACCTCCT TGATGGTCGG TTTGATAAGA CAGGGCTGTT CCTGTGAAGT CTGTGGGTTC 
TCATGTCATA TAACTTGTGT GAACAAAGCC CCGACGGTTT GCCCAGTTCC TCCTGAGCAA 
ACTAAAGGTC CACTGGGCAT AGACCCCCAG AAGGGAGTTG GGACGGCGTA TGAGGGTCAT 
GTCAGGATCC CTAAGCCGGC GGGAGTGAAG AAAGGGTGGC AGAGAGCACT GGCTGTGGTC 
TGCGACTTCA AACTGTTTCT TTATGATATC GCAGAGGGAA AAGCATCGCA GCCCACCTCC 
GTCATTAGTC AAGTGATTGA CATGAGAGAT GAAGAATTTT CTGTGAGTTC AGTCTTAGCT 
TCTGATGTTA TTCACGCAAG TCGAAAAGAT ATCCCCTGTA TATTCAGGGT CACAGCTTCC 
CAGCTCTCAG CACCTAGTAA CAAATGTTCC ATCCTGATGC TAGCAGACAG CGAGAATGAG 
AGGAGTAAGT GGGTGGGAGT GCTGAGTGAA CTCCACAAGA TTCTGAAGAA AAACAAGTTC 
AGAGACCGCT CAGTGTATGT CCCCAAGGAG GCGTACGACA GCACGCTACC CCTCATTAAG 
ACAACGCAGG CCGCTGCAAT CATAGATCAT GAACGGATAG CTTTGGGAAA TGAAGAAGGA 
TTATTTGTTG TGCATGTCAC CAAAGATGAG ATTGTTAGAG TTGGTGATAA TAAGAAAATC 
CATCAGATCG AACTCATTCC AAGTGACCAG CTTGTCGCTG TGATCTCAGG CCGAAATCGC 
CACGTCCGAC TTTTTCCTAT GTCAGCCTTG GATGGGCGAG AGACGGATTT TTATAAACTG 
GCAGAGACTA AAGGGTGTCA GACCATAGCT GCTGGGAAAG TGCGCCACGG AGCTCTGTCC 
TGCCTGTGTG TGGCCATGAA GAGACAGGTC CTCTGCTATG AACTGTTCCA AAGCAAGACC 
AGGCACAGGA AGTTTAAGGA GATTCAAGTC CCATGTAATG TCCAGTGGAT GGCCATCTTC 
AGTGAGCATC TCTGTGTGGG ATTCCAGTCA GGATTTCTCA GATACCCCCT GAATGGAGAA 
GGAGGTCCAT GCAATATGCT CCATTCAAAT GACCATACAC TGTCATTCAT TTCACACCAA 
CCAATGGACG CTCTCTGTGC AGTTGAGATC TCCAACAAAG AGTACTTGCT GTGTTTTAAC 
AGCATTGGGA TATACACAGA CTGCCAGGGC CGAAGATCCA GACAGCAGGA ACTGATGTGG 
CCAGCAAATC CTTCCTCCTG TTGTTACAAT GCTCCATATC TCTCCGTGTA TAGTGAAAAC 
GCAGTTGATA TCTTTGATGT AAACTCCATG GAATGGATAC AGACTCTTCC TCTCAAAAAG 
GTCCGACCTT TAAACACTGA AGGATCATTA AATCTCTTAG GATTGGAGAC CATTAGATTA 
ATATATTTCA AAAATAAGAT GGCAGAAGGG GATGAACTTG TGGTTCCTGA AACATCAGAT 
AATAGTCGGA AACAAATGGT TAGAAATATC AACAACAAAC GGCGTTACTC CTTCCGAGTC 
CCTGAAGAAG AGAGGATGCA ACAGAGGAGG GAGATGCTAC GAGATCCAGA AATGAGAAAT 
AAATTAATTT CTAACCCAAC TAATTTTAAC CACATAGCAC ACATGGGTCC TGGAGATGGA 
ATACAGATCC TAAAAGACCT GCCCATGAAC CCACGGCCTC AGGAAAGCCG GACAGTATTC 
AGTGGCTCCG TCAGTATTCC CTCCATCACC AAGTCCCGCC CTGAGCCAGG CCGCTCCATG 
AGTGCCAGCA GCGGCCTGTC TGCACGGTCG TCAGCCCAGA ATGGCAGTGC ACTAAAGAGG 
GAATTCTCTG GAGGAAGCTA TAACACAAAG CGACAGCCCA TGCCCTCTCC CTCGGAGGGC 
TCTTTGTCAT CAGGAGGCAT GGACCAAGGA AGTGACGCCC CTGCGAGGGA CTATGATGGA 
GAGGACTCTG ATTCTCCAAG GCATTCCACA GCTTCCAACA GCTCTAACCT GAGCAGCCCC 
CCAAGTCCCA TTTCACCCCA AAAGACCAAG AGCCTCTCCC TGGAGAGCAC AGACCGCGGG 
AGCTGGGATC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175623.1
CDS	1611..6545
Translation	MKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSLSSSSSSLIDFLDDRSPSCTPASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_017320134.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320134.1

ORF Insert Sequence:

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGATG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGACTAACAG AATTGCACAC CCAAAAGCAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGCT GAGAGCTTAA GGCAGGAGCT GCGCAGAGCA 
GAAAGAGCCA AGAAAGAGCT GGAAGTTCAC ACCGAAGCTC TAATTGCTGA AGCATCAAAG 
GACAAGAAGC TCCGAGAGCA GAGCGAACAT TATTCTAAGC AACTGGAAAA CGAGTTGGAG 
GGCCTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAATAT GCAGCATAGA ACATCAGCAA 
GAGATAACTA AACTGAAAAC TGACTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAAATT 
TCTAAAAGAG AAGGAATTCA CGCAAGTGAG ATTAAAAACC TGAAGAAGGA GCTGCATGAC 
TCAGAAGGCC AGCAGCTTGC TCTCAACAAA GAGATTCTGG TTTTAAAAGA CAAATTGGAA 
AAAACCAGGA GAGAAAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAACAACAA 
TATGAACGGG AAAAAGTATT ATTAACTGAA GAGAATAAAA AGTTAACGAG TGAACTCGAT 
AAGCTGACTT CACTGTATGA GAGCTTAAGT TTGCGCAACC AGCACTTAGA AGAAGAAGTC 
AAAGATCTAG CAGATAAGAA GGAATCAGTT GCCCACTGGG AAGCCCAAAT CACAGAGATA 
ATTCAGTGGG TGAGCGATGA GAAGGATGCA CGAGGCTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AGTTGGAAGC ATTAAGAAAT TCCAGCTTGG GGACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCAAAACTG GACATGTCGG CTAGACTAGA GTTGCAGTCT 
GCTCTGGATG CAGAAATAAG AGCTAAGCAG GCCATCCAGG AAGAGCTGAA TAAAGTCAAA 
GCATCTAACA TCTTAACAGA ATGTAAACTG AAAGATTCAG AGAAGAAGAA CTTGGAACTG 
TTGTCCGAAA TCGAGCAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GACCAATTTG AAGATTCCCT TTCTTCTTCC TCATCCTCAC TGATTGATTT TTTGGATGAC 
CGCTCTCCGT CCTGTACTCC AGCTAGCAAA GGCAGACGTA TTGCAGACTG TGCTCCACTT 
CCAGCTCACA CACCAACCTT AAGGAAAAAG GGATGCCCGG CTTCAACTGG CTTTCCACCT 
AAGCGCAAGA CTCATCAATT TTTCGTGAAG TCTTTCACTG CCCCTACCAA GTGTCACCAG 
TGCACCTCCT TGATGGTCGG TTTGATAAGA CAGGGCTGTT CCTGTGAAGT CTGTGGGTTC 
TCATGTCATA TAACTTGTGT GAACAAAGCC CCGACGGTTT GCCCAGTTCC TCCTGAGCAA 
ACTAAAGGTC CACTGGGCAT AGACCCCCAG AAGGGAGTTG GGACGGCGTA TGAGGGTCAT 
GTCAGGATCC CTAAGCCGGC GGGAGTGAAG AAAGGGTGGC AGAGAGCACT GGCTGTGGTC 
TGCGACTTCA AACTGTTTCT TTATGATATC GCAGAGGGAA AAGCATCGCA GCCCACCTCC 
GTCATTAGTC AAGTGATTGA CATGAGAGAT GAAGAATTTT CTGTGAGTTC AGTCTTAGCT 
TCTGATGTTA TTCACGCAAG TCGAAAAGAT ATCCCCTGTA TATTCAGGGT CACAGCTTCC 
CAGCTCTCAG CACCTAGTAA CAAATGTTCC ATCCTGATGC TAGCAGACAG CGAGAATGAG 
AGGAGTAAGT GGGTGGGAGT GCTGAGTGAA CTCCACAAGA TTCTGAAGAA AAACAAGTTC 
AGAGACCGCT CAGTGTATGT CCCCAAGGAG GCGTACGACA GCACGCTACC CCTCATTAAG 
ACAACGCAGG CCGCTGCAAT CATAGATCAT GAACGGATAG CTTTGGGAAA TGAAGAAGGA 
TTATTTGTTG TGCATGTCAC CAAAGATGAG ATTGTTAGAG TTGGTGATAA TAAGAAAATC 
CATCAGATCG AACTCATTCC AAGTGACCAG CTTGTCGCTG TGATCTCAGG CCGAAATCGC 
CACGTCCGAC TTTTTCCTAT GTCAGCCTTG GATGGGCGAG AGACGGATTT TTATAAACTG 
GCAGAGACTA AAGGGTGTCA GACCATAGCT GCTGGGAAAG TGCGCCACGG AGCTCTGTCC 
TGCCTGTGTG TGGCCATGAA GAGACAGGTC CTCTGCTATG AACTGTTCCA AAGCAAGACC 
AGGCACAGGA AGTTTAAGGA GATTCAAGTC CCATGTAATG TCCAGTGGAT GGCCATCTTC 
AGTGAGCATC TCTGTGTGGG ATTCCAGTCA GGATTTCTCA GATACCCCCT GAATGGAGAA 
GGAGGTCCAT GCAATATGCT CCATTCAAAT GACCATACAC TGTCATTCAT TTCACACCAA 
CCAATGGACG CTCTCTGTGC AGTTGAGATC TCCAACAAAG AGTACTTGCT GTGTTTTAAC 
AGCATTGGGA TATACACAGA CTGCCAGGGC CGAAGATCCA GACAGCAGGA ACTGATGTGG 
CCAGCAAATC CTTCCTCCTG TTGTTACAAT GCTCCATATC TCTCCGTGTA TAGTGAAAAC 
GCAGTTGATA TCTTTGATGT AAACTCCATG GAATGGATAC AGACTCTTCC TCTCAAAAAG 
GTCCGACCTT TAAACACTGA AGGATCATTA AATCTCTTAG GATTGGAGAC CATTAGATTA 
ATATATTTCA AAAATAAGAT GGCAGAAGGG GATGAACTTG TGGTTCCTGA AACATCAGAT 
AATAGTCGGA AACAAATGGT TAGAAATATC AACAACAAAC GGCGTTACTC CTTCCGAGTC 
CCTGAAGAAG AGAGGATGCA ACAGAGGAGG GAGATGCTAC GAGATCCAGA AATGAGAAAT 
AAATTAATTT CTAACCCAAC TAATTTTAAC CACATAGCAC ACATGGGTCC TGGAGATGGA 
ATACAGATCC TAAAAGACCT GCCCATGAAC CCACGGCCTC AGGAAAGCCG GACAGTATTC 
AGTGGCTCCG TCAGTATTCC CTCCATCACC AAGTCCCGCC CTGAGCCAGG CCGCTCCATG 
AGTGCCAGCA GCGGCCTGTC TGCACGGTCG TCAGCCCAGA ATGGCAGTGC ACTAAAGAGG 
GAATTCTCTG GAGGAAGCTA TAACACAAAG CGACAGCCCA TGCCCTCTCC CTCGGAGGGC 
TCTTTGTCAT CAGGAGGCAT GGACCAAGGA AGTGACGCCC CTGCGAGGGA CTATGATGGA 
GAGGACTCTG ATTCTCCAAG GCATTCCACA GCTTCCAACA GCTCTAACCT GAGCAGCCCC 
CCAAGTCCCA TTTCACCCCA AAAGACCAAG AGCCTCTCCC TGGAGAGCAC AGACCGCGGG 
AGCTGGGATC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_017320142.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175631.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OMu77099
Clone ID Related Accession (Same CDS sequence)	XM_017320142.1 , NM_001359548.1
Accession Version	XM_017320142.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGATG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGACTAACAG AATTGCACAC CCAAAAGCAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGCT GAGAGCTTAA GGCAGGAGCT GCGCAGAGCA 
GAAAGAGCCA AGAAAGAGCT GGAAGTTCAC ACCGAAGCTC TAATTGCTGA AGCATCAAAG 
GACAAGAAGC TCCGAGAGCA GAGCGAACAT TATTCTAAGC AACTGGAAAA CGAGTTGGAG 
GGCCTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAATAT GCAGCATAGA ACATCAGCAA 
GAGATAACTA AACTGAAAAC TGACTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAAATT 
TCTAAAAGAG AAGGAATTCA CGCAAGTGAG ATTAAAAACC TGAAGAAGGA GCTGCATGAC 
TCAGAAGGCC AGCAGCTTGC TCTCAACAAA GAGATTCTGG TTTTAAAAGA CAAATTGGAA 
AAAACCAGGA GAGAAAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAACAACAA 
TATGAACGGG AAAAAGTATT ATTAACTGAA GAGAATAAAA AGTTAACGAG TGAACTCGAT 
AAGCTGACTT CACTGTATGA GAGCTTAAGT TTGCGCAACC AGCACTTAGA AGAAGAAGTC 
AAAGATCTAG CAGATAAGAA GGAATCAGTT GCCCACTGGG AAGCCCAAAT CACAGAGATA 
ATTCAGTGGG TGAGCGATGA GAAGGATGCA CGAGGCTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AGTTGGAAGC ATTAAGAAAT TCCAGCTTGG GGACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCAAAACTG GACATGTCGG CTAGACTAGA GTTGCAGTCT 
GCTCTGGATG CAGAAATAAG AGCTAAGCAG GCCATCCAGG AAGAGCTGAA TAAAGTCAAA 
GCATCTAACA TCTTAACAGA ATGTAAACTG AAAGATTCAG AGAAGAAGAA CTTGGAACTG 
TTGTCCGAAA TCGAGCAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GACCAATTTG AAGATTCCCT TTCTTCTTCC TCATCCTCAC TGATTGATTT TTTGGATGAC 
ATTGCAGACT GTGCTCCACT TCCAGCTCAC ACACCAACCT TAAGGAAAAA GGGATGCCCG 
GCTTCAACTG GCTTTCCACC TAAGCGCAAG ACTCATCAAT TTTTCGTGAA GTCTTTCACT 
GCCCCTACCA AGTGTCACCA GTGCACCTCC TTGATGGTCG GTTTGATAAG ACAGGGCTGT 
TCCTGTGAAG TCTGTGGGTT CTCATGTCAT ATAACTTGTG TGAACAAAGC CCCGACGGTT 
TGCCCAGTTC CTCCTGAGCA AACTAAAGGT CCACTGGGCA TAGACCCCCA GAAGGGAGTT 
GGGACGGCGT ATGAGGGTCA TGTCAGGATC CCTAAGCCGG CGGGAGTGAA GAAAGGGTGG 
CAGAGAGCAC TGGCTGTGGT CTGCGACTTC AAACTGTTTC TTTATGATAT CGCAGAGGGA 
AAAGCATCGC AGCCCACCTC CGTCATTAGT CAAGTGATTG ACATGAGAGA TGAAGAATTT 
TCTGTGAGTT CAGTCTTAGC TTCTGATGTT ATTCACGCAA GTCGAAAAGA TATCCCCTGT 
ATATTCAGGG TCACAGCTTC CCAGCTCTCA GCACCTAGTA ACAAATGTTC CATCCTGATG 
CTAGCAGACA GCGAGAATGA GAGGAGTAAG TGGGTGGGAG TGCTGAGTGA ACTCCACAAG 
ATTCTGAAGA AAAACAAGTT CAGAGACCGC TCAGTGTATG TCCCCAAGGA GGCGTACGAC 
AGCACGCTAC CCCTCATTAA GACAACGCAG GCCGCTGCAA TCATAGATCA TGAACGGATA 
GCTTTGGGAA ATGAAGAAGG ATTATTTGTT GTGCATGTCA CCAAAGATGA GATTGTTAGA 
GTTGGTGATA ATAAGAAAAT CCATCAGATC GAACTCATTC CAAGTGACCA GCTTGTCGCT 
GTGATCTCAG GCCGAAATCG CCACGTCCGA CTTTTTCCTA TGTCAGCCTT GGATGGGCGA 
GAGACGGATT TTTATAAACT GGCAGAGACT AAAGGGTGTC AGACCATAGC TGCTGGGAAA 
GTGCGCCACG GAGCTCTGTC CTGCCTGTGT GTGGCCATGA AGAGACAGGT CCTCTGCTAT 
GAACTGTTCC AAAGCAAGAC CAGGCACAGG AAGTTTAAGG AGATTCAAGT CCCATGTAAT 
GTCCAGTGGA TGGCCATCTT CAGTGAGCAT CTCTGTGTGG GATTCCAGTC AGGATTTCTC 
AGATACCCCC TGAATGGAGA AGGAGGTCCA TGCAATATGC TCCATTCAAA TGACCATACA 
CTGTCATTCA TTTCACACCA ACCAATGGAC GCTCTCTGTG CAGTTGAGAT CTCCAACAAA 
GAGTACTTGC TGTGTTTTAA CAGCATTGGG ATATACACAG ACTGCCAGGG CCGAAGATCC 
AGACAGCAGG AACTGATGTG GCCAGCAAAT CCTTCCTCCT GTTGTTACAA TGCTCCATAT 
CTCTCCGTGT ATAGTGAAAA CGCAGTTGAT ATCTTTGATG TAAACTCCAT GGAATGGATA 
CAGACTCTTC CTCTCAAAAA GGTCCGACCT TTAAACACTG AAGGATCATT AAATCTCTTA 
GGATTGGAGA CCATTAGATT AATATATTTC AAAAATAAGA TGGCAGAAGG GGATGAACTT 
GTGGTTCCTG AAACATCAGA TAATAGTCGG AAACAAATGG TTAGAAATAT CAACAACAAA 
CGGCGTTACT CCTTCCGAGT CCCTGAAGAA GAGAGGATGC AACAGAGGAG GGAGATGCTA 
CGAGATCCAG AAATGAGAAA TAAATTAATT TCTAACCCAA CTAATTTTAA CCACATAGCA 
CACATGGGTC CTGGAGATGG AATACAGATC CTAAAAGACC TGCCCATGAA CCCACGGCCT 
CAGGAAAGCC GGACAGTATT CAGTGGCTCC GTCAGTATTC CCTCCATCAC CAAGTCCCGC 
CCTGAGCCAG GCCGCTCCAT GAGTGCCAGC AGCGGCCTGT CTGCACGGTC GTCAGCCCAG 
AATGGCAGTG CACTAAAGAG GGAATTCTCT GGAGGAAGCT ATAACACAAA GCGACAGCCC 
ATGCCCTCTC CCTCGGAGGG CTCTTTGTCA TCAGGAGGCA TGGACCAAGG AAGTGACGCC 
CCTGCGAGGG ACTATGATGG AGAGGACTCT GATTCTCCAA GGCATTCCAC AGCTTCCAAC 
AGCTCTAACC TGAGCAGCCC CCCAAGTCCC ATTTCACCCC AAAAGACCAA GAGCCTCTCC 
CTGGAGAGCA CAGACCGCGG GAGCTGGGAT CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175631.1
CDS	1611..6506
Translation	MKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSLSSSSSSLIDFLDDIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_017320142.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320142.1

ORF Insert Sequence:

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGATG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGACTAACAG AATTGCACAC CCAAAAGCAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGCT GAGAGCTTAA GGCAGGAGCT GCGCAGAGCA 
GAAAGAGCCA AGAAAGAGCT GGAAGTTCAC ACCGAAGCTC TAATTGCTGA AGCATCAAAG 
GACAAGAAGC TCCGAGAGCA GAGCGAACAT TATTCTAAGC AACTGGAAAA CGAGTTGGAG 
GGCCTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAATAT GCAGCATAGA ACATCAGCAA 
GAGATAACTA AACTGAAAAC TGACTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAAATT 
TCTAAAAGAG AAGGAATTCA CGCAAGTGAG ATTAAAAACC TGAAGAAGGA GCTGCATGAC 
TCAGAAGGCC AGCAGCTTGC TCTCAACAAA GAGATTCTGG TTTTAAAAGA CAAATTGGAA 
AAAACCAGGA GAGAAAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAACAACAA 
TATGAACGGG AAAAAGTATT ATTAACTGAA GAGAATAAAA AGTTAACGAG TGAACTCGAT 
AAGCTGACTT CACTGTATGA GAGCTTAAGT TTGCGCAACC AGCACTTAGA AGAAGAAGTC 
AAAGATCTAG CAGATAAGAA GGAATCAGTT GCCCACTGGG AAGCCCAAAT CACAGAGATA 
ATTCAGTGGG TGAGCGATGA GAAGGATGCA CGAGGCTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AGTTGGAAGC ATTAAGAAAT TCCAGCTTGG GGACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCAAAACTG GACATGTCGG CTAGACTAGA GTTGCAGTCT 
GCTCTGGATG CAGAAATAAG AGCTAAGCAG GCCATCCAGG AAGAGCTGAA TAAAGTCAAA 
GCATCTAACA TCTTAACAGA ATGTAAACTG AAAGATTCAG AGAAGAAGAA CTTGGAACTG 
TTGTCCGAAA TCGAGCAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GACCAATTTG AAGATTCCCT TTCTTCTTCC TCATCCTCAC TGATTGATTT TTTGGATGAC 
ATTGCAGACT GTGCTCCACT TCCAGCTCAC ACACCAACCT TAAGGAAAAA GGGATGCCCG 
GCTTCAACTG GCTTTCCACC TAAGCGCAAG ACTCATCAAT TTTTCGTGAA GTCTTTCACT 
GCCCCTACCA AGTGTCACCA GTGCACCTCC TTGATGGTCG GTTTGATAAG ACAGGGCTGT 
TCCTGTGAAG TCTGTGGGTT CTCATGTCAT ATAACTTGTG TGAACAAAGC CCCGACGGTT 
TGCCCAGTTC CTCCTGAGCA AACTAAAGGT CCACTGGGCA TAGACCCCCA GAAGGGAGTT 
GGGACGGCGT ATGAGGGTCA TGTCAGGATC CCTAAGCCGG CGGGAGTGAA GAAAGGGTGG 
CAGAGAGCAC TGGCTGTGGT CTGCGACTTC AAACTGTTTC TTTATGATAT CGCAGAGGGA 
AAAGCATCGC AGCCCACCTC CGTCATTAGT CAAGTGATTG ACATGAGAGA TGAAGAATTT 
TCTGTGAGTT CAGTCTTAGC TTCTGATGTT ATTCACGCAA GTCGAAAAGA TATCCCCTGT 
ATATTCAGGG TCACAGCTTC CCAGCTCTCA GCACCTAGTA ACAAATGTTC CATCCTGATG 
CTAGCAGACA GCGAGAATGA GAGGAGTAAG TGGGTGGGAG TGCTGAGTGA ACTCCACAAG 
ATTCTGAAGA AAAACAAGTT CAGAGACCGC TCAGTGTATG TCCCCAAGGA GGCGTACGAC 
AGCACGCTAC CCCTCATTAA GACAACGCAG GCCGCTGCAA TCATAGATCA TGAACGGATA 
GCTTTGGGAA ATGAAGAAGG ATTATTTGTT GTGCATGTCA CCAAAGATGA GATTGTTAGA 
GTTGGTGATA ATAAGAAAAT CCATCAGATC GAACTCATTC CAAGTGACCA GCTTGTCGCT 
GTGATCTCAG GCCGAAATCG CCACGTCCGA CTTTTTCCTA TGTCAGCCTT GGATGGGCGA 
GAGACGGATT TTTATAAACT GGCAGAGACT AAAGGGTGTC AGACCATAGC TGCTGGGAAA 
GTGCGCCACG GAGCTCTGTC CTGCCTGTGT GTGGCCATGA AGAGACAGGT CCTCTGCTAT 
GAACTGTTCC AAAGCAAGAC CAGGCACAGG AAGTTTAAGG AGATTCAAGT CCCATGTAAT 
GTCCAGTGGA TGGCCATCTT CAGTGAGCAT CTCTGTGTGG GATTCCAGTC AGGATTTCTC 
AGATACCCCC TGAATGGAGA AGGAGGTCCA TGCAATATGC TCCATTCAAA TGACCATACA 
CTGTCATTCA TTTCACACCA ACCAATGGAC GCTCTCTGTG CAGTTGAGAT CTCCAACAAA 
GAGTACTTGC TGTGTTTTAA CAGCATTGGG ATATACACAG ACTGCCAGGG CCGAAGATCC 
AGACAGCAGG AACTGATGTG GCCAGCAAAT CCTTCCTCCT GTTGTTACAA TGCTCCATAT 
CTCTCCGTGT ATAGTGAAAA CGCAGTTGAT ATCTTTGATG TAAACTCCAT GGAATGGATA 
CAGACTCTTC CTCTCAAAAA GGTCCGACCT TTAAACACTG AAGGATCATT AAATCTCTTA 
GGATTGGAGA CCATTAGATT AATATATTTC AAAAATAAGA TGGCAGAAGG GGATGAACTT 
GTGGTTCCTG AAACATCAGA TAATAGTCGG AAACAAATGG TTAGAAATAT CAACAACAAA 
CGGCGTTACT CCTTCCGAGT CCCTGAAGAA GAGAGGATGC AACAGAGGAG GGAGATGCTA 
CGAGATCCAG AAATGAGAAA TAAATTAATT TCTAACCCAA CTAATTTTAA CCACATAGCA 
CACATGGGTC CTGGAGATGG AATACAGATC CTAAAAGACC TGCCCATGAA CCCACGGCCT 
CAGGAAAGCC GGACAGTATT CAGTGGCTCC GTCAGTATTC CCTCCATCAC CAAGTCCCGC 
CCTGAGCCAG GCCGCTCCAT GAGTGCCAGC AGCGGCCTGT CTGCACGGTC GTCAGCCCAG 
AATGGCAGTG CACTAAAGAG GGAATTCTCT GGAGGAAGCT ATAACACAAA GCGACAGCCC 
ATGCCCTCTC CCTCGGAGGG CTCTTTGTCA TCAGGAGGCA TGGACCAAGG AAGTGACGCC 
CCTGCGAGGG ACTATGATGG AGAGGACTCT GATTCTCCAA GGCATTCCAC AGCTTCCAAC 
AGCTCTAACC TGAGCAGCCC CCCAAGTCCC ATTTCACCCC AAAAGACCAA GAGCCTCTCC 
CTGGAGAGCA CAGACCGCGG GAGCTGGGAT CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_017320145.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175634.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X24, mRNA.

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CloneID	OMu77100
Clone ID Related Accession (Same CDS sequence)	XM_017320145.1
Accession Version	XM_017320145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGCTG GAAGTTCACA CCGAAGCTCT AATTGCTGAA 
GCATCAAAGG ACAAGAAGCT CCGAGAGCAG AGCGAACATT ATTCTAAGCA ACTGGAAAAC 
GAGTTGGAGG GCCTGAAGCA AAAACAAATT AGTTACTCAC CAGGAATATG CAGCATAGAA 
CATCAGCAAG AGATAACTAA ACTGAAAACT GACTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAAATTT CTAAAAGAGA AGGAATTCAC GCAAGTGAGA TTAAAAACCT GAAGAAGGAG 
CTGCATGACT CAGAAGGCCA GCAGCTTGCT CTCAACAAAG AGATTCTGGT TTTAAAAGAC 
AAATTGGAAA AAACCAGGAG AGAAAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAACAACAAT ATGAACGGGA AAAAGTATTA TTAACTGAAG AGAATAAAAA GTTAACGAGT 
GAACTCGATA AGCTGACTTC ACTGTATGAG AGCTTAAGTT TGCGCAACCA GCACTTAGAA 
GAAGAAGTCA AAGATCTAGC AGATAAGAAG GAATCAGTTG CCCACTGGGA AGCCCAAATC 
ACAGAGATAA TTCAGTGGGT GAGCGATGAG AAGGATGCAC GAGGCTATCT TCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA GTTGGAAGCA TTAAGAAATT CCAGCTTGGG GACACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCAAAACTGG ACATGTCGGC TAGACTAGAG 
TTGCAGTCTG CTCTGGATGC AGAAATAAGA GCTAAGCAGG CCATCCAGGA AGAGCTGAAT 
AAAGTCAAAG CATCTAACAT CTTAACAGAA TGTAAACTGA AAGATTCAGA GAAGAAGAAC 
TTGGAACTGT TGTCCGAAAT CGAGCAGCTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGCTAGCA AAGGCAGACG TATTGCAGAC TGTGCTCCAC TTCCAGCTCA CACACCAACC 
TTAAGGAAAA AGGGATGCCC GGCTTCAACT GGCTTTCCAC CTAAGCGCAA GACTCATCAA 
TTTTTCGTGA AGTCTTTCAC TGCCCCTACC AAGTGTCACC AGTGCACCTC CTTGATGGTC 
GGTTTGATAA GACAGGGCTG TTCCTGTGAA GTCTGTGGGT TCTCATGTCA TATAACTTGT 
GTGAACAAAG CCCCGACGGT TTGCCCAGTT CCTCCTGAGC AAACTAAAGG TCCACTGGGC 
ATAGACCCCC AGAAGGGAGT TGGGACGGCG TATGAGGGTC ATGTCAGGAT CCCTAAGCCG 
GCGGGAGTGA AGAAAGGGTG GCAGAGAGCA CTGGCTGTGG TCTGCGACTT CAAACTGTTT 
CTTTATGATA TCGCAGAGGG AAAAGCATCG CAGCCCACCT CCGTCATTAG TCAAGTGATT 
GACATGAGAG ATGAAGAATT TTCTGTGAGT TCAGTCTTAG CTTCTGATGT TATTCACGCA 
AGTCGAAAAG ATATCCCCTG TATATTCAGG GTCACAGCTT CCCAGCTCTC AGCACCTAGT 
AACAAATGTT CCATCCTGAT GCTAGCAGAC AGCGAGAATG AGAGGAGTAA GTGGGTGGGA 
GTGCTGAGTG AACTCCACAA GATTCTGAAG AAAAACAAGT TCAGAGACCG CTCAGTGTAT 
GTCCCCAAGG AGGCGTACGA CAGCACGCTA CCCCTCATTA AGACAACGCA GGCCGCTGCA 
ATCATAGATC ATGAACGGAT AGCTTTGGGA AATGAAGAAG GATTATTTGT TGTGCATGTC 
ACCAAAGATG AGATTGTTAG AGTTGGTGAT AATAAGAAAA TCCATCAGAT CGAACTCATT 
CCAAGTGACC AGCTTGTCGC TGTGATCTCA GGCCGAAATC GCCACGTCCG ACTTTTTCCT 
ATGTCAGCCT TGGATGGGCG AGAGACGGAT TTTTATAAAC TGGCAGAGAC TAAAGGGTGT 
CAGACCATAG CTGCTGGGAA AGTGCGCCAC GGAGCTCTGT CCTGCCTGTG TGTGGCCATG 
AAGAGACAGG TCCTCTGCTA TGAACTGTTC CAAAGCAAGA CCAGGCACAG GAAGTTTAAG 
GAGATTCAAG TCCCATGTAA TGTCCAGTGG ATGGCCATCT TCAGTGAGCA TCTCTGTGTG 
GGATTCCAGT CAGGATTTCT CAGATACCCC CTGAATGGAG AAGGAGGTCC ATGCAATATG 
CTCCATTCAA ATGACCATAC ACTGTCATTC ATTTCACACC AACCAATGGA CGCTCTCTGT 
GCAGTTGAGA TCTCCAACAA AGAGTACTTG CTGTGTTTTA ACAGCATTGG GATATACACA 
GACTGCCAGG GCCGAAGATC CAGACAGCAG GAACTGATGT GGCCAGCAAA TCCTTCCTCC 
TGTTGTTACA ATGCTCCATA TCTCTCCGTG TATAGTGAAA ACGCAGTTGA TATCTTTGAT 
GTAAACTCCA TGGAATGGAT ACAGACTCTT CCTCTCAAAA AGGTCCGACC TTTAAACACT 
GAAGGATCAT TAAATCTCTT AGGATTGGAG ACCATTAGAT TAATATATTT CAAAAATAAG 
ATGGCAGAAG GGGATGAACT TGTGGTTCCT GAAACATCAG ATAATAGTCG GAAACAAATG 
GTTAGAAATA TCAACAACAA ACGGCGTTAC TCCTTCCGAG TCCCTGAAGA AGAGAGGATG 
CAACAGAGGA GGGAGATGCT ACGAGATCCA GAAATGAGAA ATAAATTAAT TTCTAACCCA 
ACTAATTTTA ACCACATAGC ACACATGGGT CCTGGAGATG GAATACAGAT CCTAAAAGAC 
CTGCCCATGA ACCCACGGCC TCAGGAAAGC CGGACAGTAT TCAGTGGCTC CGTCAGTATT 
CCCTCCATCA CCAAGTCCCG CCCTGAGCCA GGCCGCTCCA TGAGTGCCAG CAGCGGCCTG 
TCTGCACGGT CGTCAGCCCA GAATGGCAGT GCACTAAAGA GGGAATTCTC TGGAGGAAGC 
TATAACACAA AGCGACAGCC CATGCCCTCT CCCTCGGAGG GCTCTTTGTC ATCAGGAGGC 
ATGGACCAAG GAAGTGACGC CCCTGCGAGG GACTATGATG GAGAGGACTC TGATTCTCCA 
AGGCATTCCA CAGCTTCCAA CAGCTCTAAC CTGAGCAGCC CCCCAAGTCC CATTTCACCC 
CAAAAGACCA AGAGCCTCTC CCTGGAGAGC ACAGACCGCG GGAGCTGGGA TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175634.1
CDS	1141..5997
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKLEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_017320145.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320145.1

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGCTG GAAGTTCACA CCGAAGCTCT AATTGCTGAA 
GCATCAAAGG ACAAGAAGCT CCGAGAGCAG AGCGAACATT ATTCTAAGCA ACTGGAAAAC 
GAGTTGGAGG GCCTGAAGCA AAAACAAATT AGTTACTCAC CAGGAATATG CAGCATAGAA 
CATCAGCAAG AGATAACTAA ACTGAAAACT GACTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAAATTT CTAAAAGAGA AGGAATTCAC GCAAGTGAGA TTAAAAACCT GAAGAAGGAG 
CTGCATGACT CAGAAGGCCA GCAGCTTGCT CTCAACAAAG AGATTCTGGT TTTAAAAGAC 
AAATTGGAAA AAACCAGGAG AGAAAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAACAACAAT ATGAACGGGA AAAAGTATTA TTAACTGAAG AGAATAAAAA GTTAACGAGT 
GAACTCGATA AGCTGACTTC ACTGTATGAG AGCTTAAGTT TGCGCAACCA GCACTTAGAA 
GAAGAAGTCA AAGATCTAGC AGATAAGAAG GAATCAGTTG CCCACTGGGA AGCCCAAATC 
ACAGAGATAA TTCAGTGGGT GAGCGATGAG AAGGATGCAC GAGGCTATCT TCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA GTTGGAAGCA TTAAGAAATT CCAGCTTGGG GACACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCAAAACTGG ACATGTCGGC TAGACTAGAG 
TTGCAGTCTG CTCTGGATGC AGAAATAAGA GCTAAGCAGG CCATCCAGGA AGAGCTGAAT 
AAAGTCAAAG CATCTAACAT CTTAACAGAA TGTAAACTGA AAGATTCAGA GAAGAAGAAC 
TTGGAACTGT TGTCCGAAAT CGAGCAGCTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGCTAGCA AAGGCAGACG TATTGCAGAC TGTGCTCCAC TTCCAGCTCA CACACCAACC 
TTAAGGAAAA AGGGATGCCC GGCTTCAACT GGCTTTCCAC CTAAGCGCAA GACTCATCAA 
TTTTTCGTGA AGTCTTTCAC TGCCCCTACC AAGTGTCACC AGTGCACCTC CTTGATGGTC 
GGTTTGATAA GACAGGGCTG TTCCTGTGAA GTCTGTGGGT TCTCATGTCA TATAACTTGT 
GTGAACAAAG CCCCGACGGT TTGCCCAGTT CCTCCTGAGC AAACTAAAGG TCCACTGGGC 
ATAGACCCCC AGAAGGGAGT TGGGACGGCG TATGAGGGTC ATGTCAGGAT CCCTAAGCCG 
GCGGGAGTGA AGAAAGGGTG GCAGAGAGCA CTGGCTGTGG TCTGCGACTT CAAACTGTTT 
CTTTATGATA TCGCAGAGGG AAAAGCATCG CAGCCCACCT CCGTCATTAG TCAAGTGATT 
GACATGAGAG ATGAAGAATT TTCTGTGAGT TCAGTCTTAG CTTCTGATGT TATTCACGCA 
AGTCGAAAAG ATATCCCCTG TATATTCAGG GTCACAGCTT CCCAGCTCTC AGCACCTAGT 
AACAAATGTT CCATCCTGAT GCTAGCAGAC AGCGAGAATG AGAGGAGTAA GTGGGTGGGA 
GTGCTGAGTG AACTCCACAA GATTCTGAAG AAAAACAAGT TCAGAGACCG CTCAGTGTAT 
GTCCCCAAGG AGGCGTACGA CAGCACGCTA CCCCTCATTA AGACAACGCA GGCCGCTGCA 
ATCATAGATC ATGAACGGAT AGCTTTGGGA AATGAAGAAG GATTATTTGT TGTGCATGTC 
ACCAAAGATG AGATTGTTAG AGTTGGTGAT AATAAGAAAA TCCATCAGAT CGAACTCATT 
CCAAGTGACC AGCTTGTCGC TGTGATCTCA GGCCGAAATC GCCACGTCCG ACTTTTTCCT 
ATGTCAGCCT TGGATGGGCG AGAGACGGAT TTTTATAAAC TGGCAGAGAC TAAAGGGTGT 
CAGACCATAG CTGCTGGGAA AGTGCGCCAC GGAGCTCTGT CCTGCCTGTG TGTGGCCATG 
AAGAGACAGG TCCTCTGCTA TGAACTGTTC CAAAGCAAGA CCAGGCACAG GAAGTTTAAG 
GAGATTCAAG TCCCATGTAA TGTCCAGTGG ATGGCCATCT TCAGTGAGCA TCTCTGTGTG 
GGATTCCAGT CAGGATTTCT CAGATACCCC CTGAATGGAG AAGGAGGTCC ATGCAATATG 
CTCCATTCAA ATGACCATAC ACTGTCATTC ATTTCACACC AACCAATGGA CGCTCTCTGT 
GCAGTTGAGA TCTCCAACAA AGAGTACTTG CTGTGTTTTA ACAGCATTGG GATATACACA 
GACTGCCAGG GCCGAAGATC CAGACAGCAG GAACTGATGT GGCCAGCAAA TCCTTCCTCC 
TGTTGTTACA ATGCTCCATA TCTCTCCGTG TATAGTGAAA ACGCAGTTGA TATCTTTGAT 
GTAAACTCCA TGGAATGGAT ACAGACTCTT CCTCTCAAAA AGGTCCGACC TTTAAACACT 
GAAGGATCAT TAAATCTCTT AGGATTGGAG ACCATTAGAT TAATATATTT CAAAAATAAG 
ATGGCAGAAG GGGATGAACT TGTGGTTCCT GAAACATCAG ATAATAGTCG GAAACAAATG 
GTTAGAAATA TCAACAACAA ACGGCGTTAC TCCTTCCGAG TCCCTGAAGA AGAGAGGATG 
CAACAGAGGA GGGAGATGCT ACGAGATCCA GAAATGAGAA ATAAATTAAT TTCTAACCCA 
ACTAATTTTA ACCACATAGC ACACATGGGT CCTGGAGATG GAATACAGAT CCTAAAAGAC 
CTGCCCATGA ACCCACGGCC TCAGGAAAGC CGGACAGTAT TCAGTGGCTC CGTCAGTATT 
CCCTCCATCA CCAAGTCCCG CCCTGAGCCA GGCCGCTCCA TGAGTGCCAG CAGCGGCCTG 
TCTGCACGGT CGTCAGCCCA GAATGGCAGT GCACTAAAGA GGGAATTCTC TGGAGGAAGC 
TATAACACAA AGCGACAGCC CATGCCCTCT CCCTCGGAGG GCTCTTTGTC ATCAGGAGGC 
ATGGACCAAG GAAGTGACGC CCCTGCGAGG GACTATGATG GAGAGGACTC TGATTCTCCA 
AGGCATTCCA CAGCTTCCAA CAGCTCTAAC CTGAGCAGCC CCCCAAGTCC CATTTCACCC 
CAAAAGACCA AGAGCCTCTC CCTGGAGAGC ACAGACCGCG GGAGCTGGGA TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_017320146.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175635.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X26, mRNA.

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CloneID	OMu77101
Clone ID Related Accession (Same CDS sequence)	NM_001359549.1 , XM_017320146.1
Accession Version	XM_017320146.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGATG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGACTAACAG AATTGCACAC CCAAAAGCAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGCT GAGAGCTTAA GGCAGGAGCT GCGCAGAGCA 
GAAAGAGCCA AGAAAGAGCT GGAAGTTCAC ACCGAAGCTC TAATTGCTGA AGCATCAAAG 
GACAAGAAGC TCCGAGAGCA GAGCGAACAT TATTCTAAGC AACTGGAAAA CGAGTTGGAG 
GGCCTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAATAT GCAGCATAGA ACATCAGCAA 
GAGATAACTA AACTGAAAAC TGACTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAAATT 
TCTAAAAGAG AAGGAATTCA CGCAAGTGAG ATTAAAAACC TGAAGAAGGA GCTGCATGAC 
TCAGAAGGCC AGCAGCTTGC TCTCAACAAA GAGATTCTGG TTTTAAAAGA CAAATTGGAA 
AAAACCAGGA GAGAAAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAACAACAA 
TATGAACGGG AAAAAGTATT ATTAACTGAA GAGAATAAAA AGTTAACGAG TGAACTCGAT 
AAGCTGACTT CACTGTATGA GAGCTTAAGT TTGCGCAACC AGCACTTAGA AGAAGAAGTC 
AAAGATCTAG CAGATAAGAA GGAATCAGTT GCCCACTGGG AAGCCCAAAT CACAGAGATA 
ATTCAGTGGG TGAGCGATGA GAAGGATGCA CGAGGCTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AGTTGGAAGC ATTAAGAAAT TCCAGCTTGG GGACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCAAAACTG GACATGTCGG CTAGACTAGA GTTGCAGTCT 
GCTCTGGATG CAGAAATAAG AGCTAAGCAG GCCATCCAGG AAGAGCTGAA TAAAGTCAAA 
GCATCTAACA TCTTAACAGA ATGTAAACTG AAAGATTCAG AGAAGAAGAA CTTGGAACTG 
TTGTCCGAAA TCGAGCAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGCTAGC 
AAAGGCAGAC GTATTGCAGA CTGTGCTCCA CTTCCAGCTC ACACACCAAC CTTAAGGAAA 
AAGGGATGCC CGGCTTCAAC TGGCTTTCCA CCTAAGCGCA AGACTCATCA ATTTTTCGTG 
AAGTCTTTCA CTGCCCCTAC CAAGTGTCAC CAGTGCACCT CCTTGATGGT CGGTTTGATA 
AGACAGGGCT GTTCCTGTGA AGTCTGTGGG TTCTCATGTC ATATAACTTG TGTGAACAAA 
GCCCCGACGG TTTGCCCAGT TCCTCCTGAG CAAACTAAAG GTCCACTGGG CATAGACCCC 
CAGAAGGGAG TTGGGACGGC GTATGAGGGT CATGTCAGGA TCCCTAAGCC GGCGGGAGTG 
AAGAAAGGGT GGCAGAGAGC ACTGGCTGTG GTCTGCGACT TCAAACTGTT TCTTTATGAT 
ATCGCAGAGG GAAAAGCATC GCAGCCCACC TCCGTCATTA GTCAAGTGAT TGACATGAGA 
GATGAAGAAT TTTCTGTGAG TTCAGTCTTA GCTTCTGATG TTATTCACGC AAGTCGAAAA 
GATATCCCCT GTATATTCAG GGTCACAGCT TCCCAGCTCT CAGCACCTAG TAACAAATGT 
TCCATCCTGA TGCTAGCAGA CAGCGAGAAT GAGAGGAGTA AGTGGGTGGG AGTGCTGAGT 
GAACTCCACA AGATTCTGAA GAAAAACAAG TTCAGAGACC GCTCAGTGTA TGTCCCCAAG 
GAGGCGTACG ACAGCACGCT ACCCCTCATT AAGACAACGC AGGCCGCTGC AATCATAGAT 
CATGAACGGA TAGCTTTGGG AAATGAAGAA GGATTATTTG TTGTGCATGT CACCAAAGAT 
GAGATTGTTA GAGTTGGTGA TAATAAGAAA ATCCATCAGA TCGAACTCAT TCCAAGTGAC 
CAGCTTGTCG CTGTGATCTC AGGCCGAAAT CGCCACGTCC GACTTTTTCC TATGTCAGCC 
TTGGATGGGC GAGAGACGGA TTTTTATAAA CTGGCAGAGA CTAAAGGGTG TCAGACCATA 
GCTGCTGGGA AAGTGCGCCA CGGAGCTCTG TCCTGCCTGT GTGTGGCCAT GAAGAGACAG 
GTCCTCTGCT ATGAACTGTT CCAAAGCAAG ACCAGGCACA GGAAGTTTAA GGAGATTCAA 
GTCCCATGTA ATGTCCAGTG GATGGCCATC TTCAGTGAGC ATCTCTGTGT GGGATTCCAG 
TCAGGATTTC TCAGATACCC CCTGAATGGA GAAGGAGGTC CATGCAATAT GCTCCATTCA 
AATGACCATA CACTGTCATT CATTTCACAC CAACCAATGG ACGCTCTCTG TGCAGTTGAG 
ATCTCCAACA AAGAGTACTT GCTGTGTTTT AACAGCATTG GGATATACAC AGACTGCCAG 
GGCCGAAGAT CCAGACAGCA GGAACTGATG TGGCCAGCAA ATCCTTCCTC CTGTTGTTAC 
AATGCTCCAT ATCTCTCCGT GTATAGTGAA AACGCAGTTG ATATCTTTGA TGTAAACTCC 
ATGGAATGGA TACAGACTCT TCCTCTCAAA AAGGTCCGAC CTTTAAACAC TGAAGGATCA 
TTAAATCTCT TAGGATTGGA GACCATTAGA TTAATATATT TCAAAAATAA GATGGCAGAA 
GGGGATGAAC TTGTGGTTCC TGAAACATCA GATAATAGTC GGAAACAAAT GGTTAGAAAT 
ATCAACAACA AACGGCGTTA CTCCTTCCGA GTCCCTGAAG AAGAGAGGAT GCAACAGAGG 
AGGGAGATGC TACGAGATCC AGAAATGAGA AATAAATTAA TTTCTAACCC AACTAATTTT 
AACCACATAG CACACATGGG TCCTGGAGAT GGAATACAGA TCCTAAAAGA CCTGCCCATG 
AACCCACGGC CTCAGGAAAG CCGGACAGTA TTCAGTGGCT CCGTCAGTAT TCCCTCCATC 
ACCAAGTCCC GCCCTGAGCC AGGCCGCTCC ATGAGTGCCA GCAGCGGCCT GTCTGCACGG 
TCGTCAGCCC AGAATGGCAG TGCACTAAAG AGGGAATTCT CTGGAGGAAG CTATAACACA 
AAGCGACAGC CCATGCCCTC TCCCTCGGAG GGCTCTTTGT CATCAGGAGG CATGGACCAA 
GGAAGTGACG CCCCTGCGAG GGACTATGAT GGAGAGGACT CTGATTCTCC AAGGCATTCC 
ACAGCTTCCA ACAGCTCTAA CCTGAGCAGC CCCCCAAGTC CCATTTCACC CCAAAAGACC 
AAGAGCCTCT CCCTGGAGAG CACAGACCGC GGGAGCTGGG ATCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175635.1
CDS	1611..6398
Translation	MKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_017320146.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320146.1

ORF Insert Sequence:

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGATG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGACTAACAG AATTGCACAC CCAAAAGCAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGCT GAGAGCTTAA GGCAGGAGCT GCGCAGAGCA 
GAAAGAGCCA AGAAAGAGCT GGAAGTTCAC ACCGAAGCTC TAATTGCTGA AGCATCAAAG 
GACAAGAAGC TCCGAGAGCA GAGCGAACAT TATTCTAAGC AACTGGAAAA CGAGTTGGAG 
GGCCTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAATAT GCAGCATAGA ACATCAGCAA 
GAGATAACTA AACTGAAAAC TGACTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAAATT 
TCTAAAAGAG AAGGAATTCA CGCAAGTGAG ATTAAAAACC TGAAGAAGGA GCTGCATGAC 
TCAGAAGGCC AGCAGCTTGC TCTCAACAAA GAGATTCTGG TTTTAAAAGA CAAATTGGAA 
AAAACCAGGA GAGAAAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAACAACAA 
TATGAACGGG AAAAAGTATT ATTAACTGAA GAGAATAAAA AGTTAACGAG TGAACTCGAT 
AAGCTGACTT CACTGTATGA GAGCTTAAGT TTGCGCAACC AGCACTTAGA AGAAGAAGTC 
AAAGATCTAG CAGATAAGAA GGAATCAGTT GCCCACTGGG AAGCCCAAAT CACAGAGATA 
ATTCAGTGGG TGAGCGATGA GAAGGATGCA CGAGGCTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AGTTGGAAGC ATTAAGAAAT TCCAGCTTGG GGACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCAAAACTG GACATGTCGG CTAGACTAGA GTTGCAGTCT 
GCTCTGGATG CAGAAATAAG AGCTAAGCAG GCCATCCAGG AAGAGCTGAA TAAAGTCAAA 
GCATCTAACA TCTTAACAGA ATGTAAACTG AAAGATTCAG AGAAGAAGAA CTTGGAACTG 
TTGTCCGAAA TCGAGCAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGCTAGC 
AAAGGCAGAC GTATTGCAGA CTGTGCTCCA CTTCCAGCTC ACACACCAAC CTTAAGGAAA 
AAGGGATGCC CGGCTTCAAC TGGCTTTCCA CCTAAGCGCA AGACTCATCA ATTTTTCGTG 
AAGTCTTTCA CTGCCCCTAC CAAGTGTCAC CAGTGCACCT CCTTGATGGT CGGTTTGATA 
AGACAGGGCT GTTCCTGTGA AGTCTGTGGG TTCTCATGTC ATATAACTTG TGTGAACAAA 
GCCCCGACGG TTTGCCCAGT TCCTCCTGAG CAAACTAAAG GTCCACTGGG CATAGACCCC 
CAGAAGGGAG TTGGGACGGC GTATGAGGGT CATGTCAGGA TCCCTAAGCC GGCGGGAGTG 
AAGAAAGGGT GGCAGAGAGC ACTGGCTGTG GTCTGCGACT TCAAACTGTT TCTTTATGAT 
ATCGCAGAGG GAAAAGCATC GCAGCCCACC TCCGTCATTA GTCAAGTGAT TGACATGAGA 
GATGAAGAAT TTTCTGTGAG TTCAGTCTTA GCTTCTGATG TTATTCACGC AAGTCGAAAA 
GATATCCCCT GTATATTCAG GGTCACAGCT TCCCAGCTCT CAGCACCTAG TAACAAATGT 
TCCATCCTGA TGCTAGCAGA CAGCGAGAAT GAGAGGAGTA AGTGGGTGGG AGTGCTGAGT 
GAACTCCACA AGATTCTGAA GAAAAACAAG TTCAGAGACC GCTCAGTGTA TGTCCCCAAG 
GAGGCGTACG ACAGCACGCT ACCCCTCATT AAGACAACGC AGGCCGCTGC AATCATAGAT 
CATGAACGGA TAGCTTTGGG AAATGAAGAA GGATTATTTG TTGTGCATGT CACCAAAGAT 
GAGATTGTTA GAGTTGGTGA TAATAAGAAA ATCCATCAGA TCGAACTCAT TCCAAGTGAC 
CAGCTTGTCG CTGTGATCTC AGGCCGAAAT CGCCACGTCC GACTTTTTCC TATGTCAGCC 
TTGGATGGGC GAGAGACGGA TTTTTATAAA CTGGCAGAGA CTAAAGGGTG TCAGACCATA 
GCTGCTGGGA AAGTGCGCCA CGGAGCTCTG TCCTGCCTGT GTGTGGCCAT GAAGAGACAG 
GTCCTCTGCT ATGAACTGTT CCAAAGCAAG ACCAGGCACA GGAAGTTTAA GGAGATTCAA 
GTCCCATGTA ATGTCCAGTG GATGGCCATC TTCAGTGAGC ATCTCTGTGT GGGATTCCAG 
TCAGGATTTC TCAGATACCC CCTGAATGGA GAAGGAGGTC CATGCAATAT GCTCCATTCA 
AATGACCATA CACTGTCATT CATTTCACAC CAACCAATGG ACGCTCTCTG TGCAGTTGAG 
ATCTCCAACA AAGAGTACTT GCTGTGTTTT AACAGCATTG GGATATACAC AGACTGCCAG 
GGCCGAAGAT CCAGACAGCA GGAACTGATG TGGCCAGCAA ATCCTTCCTC CTGTTGTTAC 
AATGCTCCAT ATCTCTCCGT GTATAGTGAA AACGCAGTTG ATATCTTTGA TGTAAACTCC 
ATGGAATGGA TACAGACTCT TCCTCTCAAA AAGGTCCGAC CTTTAAACAC TGAAGGATCA 
TTAAATCTCT TAGGATTGGA GACCATTAGA TTAATATATT TCAAAAATAA GATGGCAGAA 
GGGGATGAAC TTGTGGTTCC TGAAACATCA GATAATAGTC GGAAACAAAT GGTTAGAAAT 
ATCAACAACA AACGGCGTTA CTCCTTCCGA GTCCCTGAAG AAGAGAGGAT GCAACAGAGG 
AGGGAGATGC TACGAGATCC AGAAATGAGA AATAAATTAA TTTCTAACCC AACTAATTTT 
AACCACATAG CACACATGGG TCCTGGAGAT GGAATACAGA TCCTAAAAGA CCTGCCCATG 
AACCCACGGC CTCAGGAAAG CCGGACAGTA TTCAGTGGCT CCGTCAGTAT TCCCTCCATC 
ACCAAGTCCC GCCCTGAGCC AGGCCGCTCC ATGAGTGCCA GCAGCGGCCT GTCTGCACGG 
TCGTCAGCCC AGAATGGCAG TGCACTAAAG AGGGAATTCT CTGGAGGAAG CTATAACACA 
AAGCGACAGC CCATGCCCTC TCCCTCGGAG GGCTCTTTGT CATCAGGAGG CATGGACCAA 
GGAAGTGACG CCCCTGCGAG GGACTATGAT GGAGAGGACT CTGATTCTCC AAGGCATTCC 
ACAGCTTCCA ACAGCTCTAA CCTGAGCAGC CCCCCAAGTC CCATTTCACC CCAAAAGACC 
AAGAGCCTCT CCCTGGAGAG CACAGACCGC GGGAGCTGGG ATCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_017320148.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175637.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X17, mRNA.

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CloneID	OMu77102
Clone ID Related Accession (Same CDS sequence)	XM_017320148.1
Accession Version	XM_017320148.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACG GAAAAGGCAG ATATGGACCA GAGTGTGACT GGTGGTCCTT GGGGGTCTGT 
ATGTATGAGA TGCTTTACGG AGAAACACCA TTCTATGCAG AGTCTCTGGT GGAGACATAT 
GGAAAAATTA TGAATCACAA AGAGCGGTTT CAGTTTCCAG CCCAAGTGAC AGACGTGTCC 
GAAAATGCTA AAGATCTTAT TCGAAGACTC ATTTGTAGTA GAGAACATCG ACTTGGCCAA 
AATGGAATAG AAGACTTTAA GAAGCATCCA TTCTTCAGTG GAATTGATTG GGATAACATT 
CGGAATTGTG AAGCACCTTA CATCCCAGAA GTTAGTAGCC CGACAGATAC GTCAAATTTT 
GATGTGGATG ACGACTGTTT AAAAAATTCT GAAACAATGC CCCCACCAAC ACATACTGCA 
TTTTCTGGCC ATCATCTACC ATTTGTTGGT TTTACATATA CTAGTAGCTG TGTTCTTTCT 
GATCGGAGCT GTTTAAGAGT CACAGCAGGC CCCACCTCAT TGGACCTTGA TGTCAGTGTC 
CAGAGGACTC TAGACAATAA CTTAGCAACT GAGGCTTATG AAAGGAGAAT TAAGCGGCTT 
GAGCAGGAAA AGCTTGAACT GACTAGAAAA CTTCAAGAGT CAACACAGAC TGTTCAAGCT 
CTGCAATATT CAACTGTTGA TGGTCCACTA ACGGCAAGCA AAGATTTAGA AATTAAAAGT 
CTGAAAGAAG AGATTGAAAA ACTAAGGAAA CAAGTAGCAG AAGTGAATCA TTTGGAACAA 
CAACTTGAAG AAGCTAATTC TGTGAGACGA GAACTAGATG ATGCTTTCAG GCAAATCAAA 
GCTTCTGAAA AACAAATCAA AACTTTGCAG CAGGAAAGAG AAGAACTAAA TAAGGAACTA 
GTCCAGGCTA GTGAGCGATT AAAAAACCAA TCCAAAGAGC TGAAAGATGC ACACTGTCAG 
AGGAAACTGG CCATGCAGGA ATTCATGGAG ATCAATGAGC GACTAACAGA ATTGCACACC 
CAAAAGCAGA AACTTGCTCG CCATGTCCGA GATAAGGAAG AAGAGGTGGA CCTGGTGATG 
CAAAAAGCTG AGAGCTTAAG GCAGGAGCTG CGCAGAGCAG AAAGAGCCAA GAAAGAGCTG 
GAAGTTCACA CCGAAGCTCT AATTGCTGAA GCATCAAAGG ACAAGAAGCT CCGAGAGCAG 
AGCGAACATT ATTCTAAGCA ACTGGAAAAC GAGTTGGAGG GCCTGAAGCA AAAACAAATT 
AGTTACTCAC CAGGAATATG CAGCATAGAA CATCAGCAAG AGATAACTAA ACTGAAAACT 
GACTTGGAAA AGAAAAGTAT CTTTTATGAA GAAGAAATTT CTAAAAGAGA AGGAATTCAC 
GCAAGTGAGA TTAAAAACCT GAAGAAGGAG CTGCATGACT CAGAAGGCCA GCAGCTTGCT 
CTCAACAAAG AGATTCTGGT TTTAAAAGAC AAATTGGAAA AAACCAGGAG AGAAAGTCAA 
AGTGAAAGGG AAGAATTTGA AAATGAGTTC AAACAACAAT ATGAACGGGA AAAAGTATTA 
TTAACTGAAG AGAATAAAAA GTTAACGAGT GAACTCGATA AGCTGACTTC ACTGTATGAG 
AGCTTAAGTT TGCGCAACCA GCACTTAGAA GAAGAAGTCA AAGATCTAGC AGATAAGAAG 
GAATCAGTTG CCCACTGGGA AGCCCAAATC ACAGAGATAA TTCAGTGGGT GAGCGATGAG 
AAGGATGCAC GAGGCTATCT TCAGGCCTTA GCTTCTAAAA TGACTGAAGA GTTGGAAGCA 
TTAAGAAATT CCAGCTTGGG GACACGAGCA ACAGATATGC CCTGGAAAAT GCGTCGTTTT 
GCAAAACTGG ACATGTCGGC TAGACTAGAG TTGCAGTCTG CTCTGGATGC AGAAATAAGA 
GCTAAGCAGG CCATCCAGGA AGAGCTGAAT AAAGTCAAAG CATCTAACAT CTTAACAGAA 
TGTAAACTGA AAGATTCAGA GAAGAAGAAC TTGGAACTGT TGTCCGAAAT CGAGCAGCTG 
ATAAAGGACA CTGAAGAGCT TAGATCTGAA AAGGGTATAG AGCACCAAGA CTCACAGCAT 
TCTTTCTTGG CATTTTTGAA TACGCCTACC GATGCTCTGG ACCAATTTGA AGATTCCCTT 
TCTTCTTCCT CATCCTCACT GATTGATTTT TTGGATGACC GCTCTCCGTC CTGTACTCCA 
GCTAGCAAAG GCAGACGTAT TGCAGACTGT GCTCCACTTC CAGCTCACAC ACCAACCTTA 
AGGAAAAAGG GATGCCCGGC TTCAACTGGC TTTCCACCTA AGCGCAAGAC TCATCAATTT 
TTCGTGAAGT CTTTCACTGC CCCTACCAAG TGTCACCAGT GCACCTCCTT GATGGTCGGT 
TTGATAAGAC AGGGCTGTTC CTGTGAAGTC TGTGGGTTCT CATGTCATAT AACTTGTGTG 
AACAAAGCCC CGACGGTTTG CCCAGTTCCT CCTGAGCAAA CTAAAGGTCC ACTGGGCATA 
GACCCCCAGA AGGGAGTTGG GACGGCGTAT GAGGGTCATG TCAGGATCCC TAAGCCGGCG 
GGAGTGAAGA AAGGGTGGCA GAGAGCACTG GCTGTGGTCT GCGACTTCAA ACTGTTTCTT 
TATGATATCG CAGAGGGAAA AGCATCGCAG CCCACCTCCG TCATTAGTCA AGTGATTGAC 
ATGAGAGATG AAGAATTTTC TGTGAGTTCA GTCTTAGCTT CTGATGTTAT TCACGCAAGT 
CGAAAAGATA TCCCCTGTAT ATTCAGGGTC ACAGCTTCCC AGCTCTCAGC ACCTAGTAAC 
AAATGTTCCA TCCTGATGCT AGCAGACAGC GAGAATGAGA GGAGTAAGTG GGTGGGAGTG 
CTGAGTGAAC TCCACAAGAT TCTGAAGAAA AACAAGTTCA GAGACCGCTC AGTGTATGTC 
CCCAAGGAGG CGTACGACAG CACGCTACCC CTCATTAAGA CAACGCAGGC CGCTGCAATC 
ATAGATCATG AACGGATAGC TTTGGGAAAT GAAGAAGGAT TATTTGTTGT GCATGTCACC 
AAAGATGAGA TTGTTAGAGT TGGTGATAAT AAGAAAATCC ATCAGATCGA ACTCATTCCA 
AGTGACCAGC TTGTCGCTGT GATCTCAGGC CGAAATCGCC ACGTCCGACT TTTTCCTATG 
TCAGCCTTGG ATGGGCGAGA GACGGATTTT TATAAACTGG CAGAGACTAA AGGGTGTCAG 
ACCATAGCTG CTGGGAAAGT GCGCCACGGA GCTCTGTCCT GCCTGTGTGT GGCCATGAAG 
AGACAGGTCC TCTGCTATGA ACTGTTCCAA AGCAAGACCA GGCACAGGAA GTTTAAGGAG 
ATTCAAGTCC CATGTAATGT CCAGTGGATG GCCATCTTCA GTGAGCATCT CTGTGTGGGA 
TTCCAGTCAG GATTTCTCAG ATACCCCCTG AATGGAGAAG GAGGTCCATG CAATATGCTC 
CATTCAAATG ACCATACACT GTCATTCATT TCACACCAAC CAATGGACGC TCTCTGTGCA 
GTTGAGATCT CCAACAAAGA GTACTTGCTG TGTTTTAACA GCATTGGGAT ATACACAGAC 
TGCCAGGGCC GAAGATCCAG ACAGCAGGAA CTGATGTGGC CAGCAAATCC TTCCTCCTGT 
TGTTACAATG CTCCATATCT CTCCGTGTAT AGTGAAAACG CAGTTGATAT CTTTGATGTA 
AACTCCATGG AATGGATACA GACTCTTCCT CTCAAAAAGG TCCGACCTTT AAACACTGAA 
GGATCATTAA ATCTCTTAGG ATTGGAGACC ATTAGATTAA TATATTTCAA AAATAAGATG 
GCAGAAGGGG ATGAACTTGT GGTTCCTGAA ACATCAGATA ATAGTCGGAA ACAAATGGTT 
AGAAATATCA ACAACAAACG GCGTTACTCC TTCCGAGTCC CTGAAGAAGA GAGGATGCAA 
CAGAGGAGGG AGATGCTACG AGATCCAGAA ATGAGAAATA AATTAATTTC TAACCCAACT 
AATTTTAACC ACATAGCACA CATGGGTCCT GGAGATGGAA TACAGATCCT AAAAGACCTG 
CCCATGCCAG GTTTCCCTTA TTCACCCCCT CATCACCACT CCGGCCTGAT CTCCTCTCCG 
AGCAACTTTG AGCACATCTA TCACATGACT GTCACTTCTG CTGAACAGTT TCTCTCTCCT 
GAGTCCATAA GCCCTGGGTC CCCCCCGTCC CTGCGCTCTG CCCCTGGCAC ACCACACTCT 
GTGACTCTGA GGAACCCACG GCCTCAGGAA AGCCGGACAG TATTCAGTGG CTCCGTCAGT 
ATTCCCTCCA TCACCAAGTC CCGCCCTGAG CCAGGCCGCT CCATGAGTGC CAGCAGCGGC 
CTGTCTGCAC GGTCGTCAGC CCAGAATGGC AGTGCACTAA AGAGGGAATT CTCTGGAGGA 
AGCTATAACA CAAAGCGACA GCCCATGCCC TCTCCCTCGG AGGGCTCTTT GTCATCAGGA 
GGCATGGACC AAGGAAGTGA CGCCCCTGCG AGGGACTATG ATGGAGAGGA CTCTGATTCT 
CCAAGGCATT CCACAGCTTC CAACAGCTCT AACCTGAGCA GCCCCCCAAG TCCCATTTCA 
CCCCAAAAGA CCAAGAGCCT CTCCCTGGAG AGCACAGACC GCGGGAGCTG GGATCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175637.1
CDS	3056..7735
Translation	MEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSLSSSSSSLIDFLDDRSPSCTPASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_017320148.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320148.1

ORF Insert Sequence:

ATGGAAGACG GAAAAGGCAG ATATGGACCA GAGTGTGACT GGTGGTCCTT GGGGGTCTGT 
ATGTATGAGA TGCTTTACGG AGAAACACCA TTCTATGCAG AGTCTCTGGT GGAGACATAT 
GGAAAAATTA TGAATCACAA AGAGCGGTTT CAGTTTCCAG CCCAAGTGAC AGACGTGTCC 
GAAAATGCTA AAGATCTTAT TCGAAGACTC ATTTGTAGTA GAGAACATCG ACTTGGCCAA 
AATGGAATAG AAGACTTTAA GAAGCATCCA TTCTTCAGTG GAATTGATTG GGATAACATT 
CGGAATTGTG AAGCACCTTA CATCCCAGAA GTTAGTAGCC CGACAGATAC GTCAAATTTT 
GATGTGGATG ACGACTGTTT AAAAAATTCT GAAACAATGC CCCCACCAAC ACATACTGCA 
TTTTCTGGCC ATCATCTACC ATTTGTTGGT TTTACATATA CTAGTAGCTG TGTTCTTTCT 
GATCGGAGCT GTTTAAGAGT CACAGCAGGC CCCACCTCAT TGGACCTTGA TGTCAGTGTC 
CAGAGGACTC TAGACAATAA CTTAGCAACT GAGGCTTATG AAAGGAGAAT TAAGCGGCTT 
GAGCAGGAAA AGCTTGAACT GACTAGAAAA CTTCAAGAGT CAACACAGAC TGTTCAAGCT 
CTGCAATATT CAACTGTTGA TGGTCCACTA ACGGCAAGCA AAGATTTAGA AATTAAAAGT 
CTGAAAGAAG AGATTGAAAA ACTAAGGAAA CAAGTAGCAG AAGTGAATCA TTTGGAACAA 
CAACTTGAAG AAGCTAATTC TGTGAGACGA GAACTAGATG ATGCTTTCAG GCAAATCAAA 
GCTTCTGAAA AACAAATCAA AACTTTGCAG CAGGAAAGAG AAGAACTAAA TAAGGAACTA 
GTCCAGGCTA GTGAGCGATT AAAAAACCAA TCCAAAGAGC TGAAAGATGC ACACTGTCAG 
AGGAAACTGG CCATGCAGGA ATTCATGGAG ATCAATGAGC GACTAACAGA ATTGCACACC 
CAAAAGCAGA AACTTGCTCG CCATGTCCGA GATAAGGAAG AAGAGGTGGA CCTGGTGATG 
CAAAAAGCTG AGAGCTTAAG GCAGGAGCTG CGCAGAGCAG AAAGAGCCAA GAAAGAGCTG 
GAAGTTCACA CCGAAGCTCT AATTGCTGAA GCATCAAAGG ACAAGAAGCT CCGAGAGCAG 
AGCGAACATT ATTCTAAGCA ACTGGAAAAC GAGTTGGAGG GCCTGAAGCA AAAACAAATT 
AGTTACTCAC CAGGAATATG CAGCATAGAA CATCAGCAAG AGATAACTAA ACTGAAAACT 
GACTTGGAAA AGAAAAGTAT CTTTTATGAA GAAGAAATTT CTAAAAGAGA AGGAATTCAC 
GCAAGTGAGA TTAAAAACCT GAAGAAGGAG CTGCATGACT CAGAAGGCCA GCAGCTTGCT 
CTCAACAAAG AGATTCTGGT TTTAAAAGAC AAATTGGAAA AAACCAGGAG AGAAAGTCAA 
AGTGAAAGGG AAGAATTTGA AAATGAGTTC AAACAACAAT ATGAACGGGA AAAAGTATTA 
TTAACTGAAG AGAATAAAAA GTTAACGAGT GAACTCGATA AGCTGACTTC ACTGTATGAG 
AGCTTAAGTT TGCGCAACCA GCACTTAGAA GAAGAAGTCA AAGATCTAGC AGATAAGAAG 
GAATCAGTTG CCCACTGGGA AGCCCAAATC ACAGAGATAA TTCAGTGGGT GAGCGATGAG 
AAGGATGCAC GAGGCTATCT TCAGGCCTTA GCTTCTAAAA TGACTGAAGA GTTGGAAGCA 
TTAAGAAATT CCAGCTTGGG GACACGAGCA ACAGATATGC CCTGGAAAAT GCGTCGTTTT 
GCAAAACTGG ACATGTCGGC TAGACTAGAG TTGCAGTCTG CTCTGGATGC AGAAATAAGA 
GCTAAGCAGG CCATCCAGGA AGAGCTGAAT AAAGTCAAAG CATCTAACAT CTTAACAGAA 
TGTAAACTGA AAGATTCAGA GAAGAAGAAC TTGGAACTGT TGTCCGAAAT CGAGCAGCTG 
ATAAAGGACA CTGAAGAGCT TAGATCTGAA AAGGGTATAG AGCACCAAGA CTCACAGCAT 
TCTTTCTTGG CATTTTTGAA TACGCCTACC GATGCTCTGG ACCAATTTGA AGATTCCCTT 
TCTTCTTCCT CATCCTCACT GATTGATTTT TTGGATGACC GCTCTCCGTC CTGTACTCCA 
GCTAGCAAAG GCAGACGTAT TGCAGACTGT GCTCCACTTC CAGCTCACAC ACCAACCTTA 
AGGAAAAAGG GATGCCCGGC TTCAACTGGC TTTCCACCTA AGCGCAAGAC TCATCAATTT 
TTCGTGAAGT CTTTCACTGC CCCTACCAAG TGTCACCAGT GCACCTCCTT GATGGTCGGT 
TTGATAAGAC AGGGCTGTTC CTGTGAAGTC TGTGGGTTCT CATGTCATAT AACTTGTGTG 
AACAAAGCCC CGACGGTTTG CCCAGTTCCT CCTGAGCAAA CTAAAGGTCC ACTGGGCATA 
GACCCCCAGA AGGGAGTTGG GACGGCGTAT GAGGGTCATG TCAGGATCCC TAAGCCGGCG 
GGAGTGAAGA AAGGGTGGCA GAGAGCACTG GCTGTGGTCT GCGACTTCAA ACTGTTTCTT 
TATGATATCG CAGAGGGAAA AGCATCGCAG CCCACCTCCG TCATTAGTCA AGTGATTGAC 
ATGAGAGATG AAGAATTTTC TGTGAGTTCA GTCTTAGCTT CTGATGTTAT TCACGCAAGT 
CGAAAAGATA TCCCCTGTAT ATTCAGGGTC ACAGCTTCCC AGCTCTCAGC ACCTAGTAAC 
AAATGTTCCA TCCTGATGCT AGCAGACAGC GAGAATGAGA GGAGTAAGTG GGTGGGAGTG 
CTGAGTGAAC TCCACAAGAT TCTGAAGAAA AACAAGTTCA GAGACCGCTC AGTGTATGTC 
CCCAAGGAGG CGTACGACAG CACGCTACCC CTCATTAAGA CAACGCAGGC CGCTGCAATC 
ATAGATCATG AACGGATAGC TTTGGGAAAT GAAGAAGGAT TATTTGTTGT GCATGTCACC 
AAAGATGAGA TTGTTAGAGT TGGTGATAAT AAGAAAATCC ATCAGATCGA ACTCATTCCA 
AGTGACCAGC TTGTCGCTGT GATCTCAGGC CGAAATCGCC ACGTCCGACT TTTTCCTATG 
TCAGCCTTGG ATGGGCGAGA GACGGATTTT TATAAACTGG CAGAGACTAA AGGGTGTCAG 
ACCATAGCTG CTGGGAAAGT GCGCCACGGA GCTCTGTCCT GCCTGTGTGT GGCCATGAAG 
AGACAGGTCC TCTGCTATGA ACTGTTCCAA AGCAAGACCA GGCACAGGAA GTTTAAGGAG 
ATTCAAGTCC CATGTAATGT CCAGTGGATG GCCATCTTCA GTGAGCATCT CTGTGTGGGA 
TTCCAGTCAG GATTTCTCAG ATACCCCCTG AATGGAGAAG GAGGTCCATG CAATATGCTC 
CATTCAAATG ACCATACACT GTCATTCATT TCACACCAAC CAATGGACGC TCTCTGTGCA 
GTTGAGATCT CCAACAAAGA GTACTTGCTG TGTTTTAACA GCATTGGGAT ATACACAGAC 
TGCCAGGGCC GAAGATCCAG ACAGCAGGAA CTGATGTGGC CAGCAAATCC TTCCTCCTGT 
TGTTACAATG CTCCATATCT CTCCGTGTAT AGTGAAAACG CAGTTGATAT CTTTGATGTA 
AACTCCATGG AATGGATACA GACTCTTCCT CTCAAAAAGG TCCGACCTTT AAACACTGAA 
GGATCATTAA ATCTCTTAGG ATTGGAGACC ATTAGATTAA TATATTTCAA AAATAAGATG 
GCAGAAGGGG ATGAACTTGT GGTTCCTGAA ACATCAGATA ATAGTCGGAA ACAAATGGTT 
AGAAATATCA ACAACAAACG GCGTTACTCC TTCCGAGTCC CTGAAGAAGA GAGGATGCAA 
CAGAGGAGGG AGATGCTACG AGATCCAGAA ATGAGAAATA AATTAATTTC TAACCCAACT 
AATTTTAACC ACATAGCACA CATGGGTCCT GGAGATGGAA TACAGATCCT AAAAGACCTG 
CCCATGCCAG GTTTCCCTTA TTCACCCCCT CATCACCACT CCGGCCTGAT CTCCTCTCCG 
AGCAACTTTG AGCACATCTA TCACATGACT GTCACTTCTG CTGAACAGTT TCTCTCTCCT 
GAGTCCATAA GCCCTGGGTC CCCCCCGTCC CTGCGCTCTG CCCCTGGCAC ACCACACTCT 
GTGACTCTGA GGAACCCACG GCCTCAGGAA AGCCGGACAG TATTCAGTGG CTCCGTCAGT 
ATTCCCTCCA TCACCAAGTC CCGCCCTGAG CCAGGCCGCT CCATGAGTGC CAGCAGCGGC 
CTGTCTGCAC GGTCGTCAGC CCAGAATGGC AGTGCACTAA AGAGGGAATT CTCTGGAGGA 
AGCTATAACA CAAAGCGACA GCCCATGCCC TCTCCCTCGG AGGGCTCTTT GTCATCAGGA 
GGCATGGACC AAGGAAGTGA CGCCCCTGCG AGGGACTATG ATGGAGAGGA CTCTGATTCT 
CCAAGGCATT CCACAGCTTC CAACAGCTCT AACCTGAGCA GCCCCCCAAG TCCCATTTCA 
CCCCAAAAGA CCAAGAGCCT CTCCCTGGAG AGCACAGACC GCGGGAGCTG GGATCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_030253509.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109369.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X12, mRNA.

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CloneID	OMu85130
Clone ID Related Accession (Same CDS sequence)	XM_030253509.1
Accession Version	XM_030253509.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGATG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGACTAACAG AATTGCACAC CCAAAAGCAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGCT GAGAGCTTAA GGCAGGAGCT GCGCAGAGCA 
GAAAGAGCCA AGAAAGAGCT GGAAGTTCAC ACCGAAGCTC TAATTGCTGA AGCATCAAAG 
GACAAGAAGC TCCGAGAGCA GAGCGAACAT TATTCTAAGC AACTGGAAAA CGAGTTGGAG 
GGCCTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAATAT GCAGCATAGA ACATCAGCAA 
GAGATAACTA AACTGAAAAC TGACTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAAATT 
TCTAAAAGAG AAGGAATTCA CGCAAGTGAG ATTAAAAACC TGAAGAAGGA GCTGCATGAC 
TCAGAAGGCC AGCAGCTTGC TCTCAACAAA GAGATTCTGG TTTTAAAAGA CAAATTGGAA 
AAAACCAGGA GAGAAAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAACAACAA 
TATGAACGGG AAAAAGTATT ATTAACTGAA GAGAATAAAA AGTTAACGAG TGAACTCGAT 
AAGCTGACTT CACTGTATGA GAGCTTAAGT TTGCGCAACC AGCACTTAGA AGAAGAAGTC 
AAAGATCTAG CAGATAAGAA GGAATCAGTT GCCCACTGGG AAGCCCAAAT CACAGAGATA 
ATTCAGTGGG TGAGCGATGA GAAGGATGCA CGAGGCTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AGTTGGAAGC ATTAAGAAAT TCCAGCTTGG GGACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCAAAACTG GACATGTCGG CTAGACTAGA GTTGCAGTCT 
GCTCTGGATG CAGAAATAAG AGCTAAGCAG GCCATCCAGG AAGAGCTGAA TAAAGTCAAA 
GCATCTAACA TCTTAACAGA ATGTAAACTG AAAGATTCAG AGAAGAAGAA CTTGGAACTG 
TTGTCCGAAA TCGAGCAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GACCAATTTG AACGCTCTCC GTCCTGTACT CCAGCTAGCA AAGGCAGACG TATTGCAGAC 
TGTGCTCCAC TTCCAGCTCA CACACCAACC TTAAGGAAAA AGGGATGCCC GGCTTCAACT 
GGCTTTCCAC CTAAGCGCAA GACTCATCAA TTTTTCGTGA AGTCTTTCAC TGCCCCTACC 
AAGTGTCACC AGTGCACCTC CTTGATGGTC GGTTTGATAA GACAGGGCTG TTCCTGTGAA 
GTCTGTGGGT TCTCATGTCA TATAACTTGT GTGAACAAAG CCCCGACGGT TTGCCCAGTT 
CCTCCTGAGC AAACTAAAGG TCCACTGGGC ATAGACCCCC AGAAGGGAGT TGGGACGGCG 
TATGAGGGTC ATGTCAGGAT CCCTAAGCCG GCGGGAGTGA AGAAAGGGTG GCAGAGAGCA 
CTGGCTGTGG TCTGCGACTT CAAACTGTTT CTTTATGATA TCGCAGAGGG AAAAGCATCG 
CAGCCCACCT CCGTCATTAG TCAAGTGATT GACATGAGAG ATGAAGAATT TTCTGTGAGT 
TCAGTCTTAG CTTCTGATGT TATTCACGCA AGTCGAAAAG ATATCCCCTG TATATTCAGG 
GTCACAGCTT CCCAGCTCTC AGCACCTAGT AACAAATGTT CCATCCTGAT GCTAGCAGAC 
AGCGAGAATG AGAGGAGTAA GTGGGTGGGA GTGCTGAGTG AACTCCACAA GATTCTGAAG 
AAAAACAAGT TCAGAGACCG CTCAGTGTAT GTCCCCAAGG AGGCGTACGA CAGCACGCTA 
CCCCTCATTA AGACAACGCA GGCCGCTGCA ATCATAGATC ATGAACGGAT AGCTTTGGGA 
AATGAAGAAG GATTATTTGT TGTGCATGTC ACCAAAGATG AGATTGTTAG AGTTGGTGAT 
AATAAGAAAA TCCATCAGAT CGAACTCATT CCAAGTGACC AGCTTGTCGC TGTGATCTCA 
GGCCGAAATC GCCACGTCCG ACTTTTTCCT ATGTCAGCCT TGGATGGGCG AGAGACGGAT 
TTTTATAAAC TGGCAGAGAC TAAAGGGTGT CAGACCATAG CTGCTGGGAA AGTGCGCCAC 
GGAGCTCTGT CCTGCCTGTG TGTGGCCATG AAGAGACAGG TCCTCTGCTA TGAACTGTTC 
CAAAGCAAGA CCAGGCACAG GAAGTTTAAG GAGATTCAAG TCCCATGTAA TGTCCAGTGG 
ATGGCCATCT TCAGTGAGCA TCTCTGTGTG GGATTCCAGT CAGGATTTCT CAGATACCCC 
CTGAATGGAG AAGGAGGTCC ATGCAATATG CTCCATTCAA ATGACCATAC ACTGTCATTC 
ATTTCACACC AACCAATGGA CGCTCTCTGT GCAGTTGAGA TCTCCAACAA AGAGTACTTG 
CTGTGTTTTA ACAGCATTGG GATATACACA GACTGCCAGG GCCGAAGATC CAGACAGCAG 
GAACTGATGT GGCCAGCAAA TCCTTCCTCC TGTTGTTACA ATGCTCCATA TCTCTCCGTG 
TATAGTGAAA ACGCAGTTGA TATCTTTGAT GTAAACTCCA TGGAATGGAT ACAGACTCTT 
CCTCTCAAAA AGGTCCGACC TTTAAACACT GAAGGATCAT TAAATCTCTT AGGATTGGAG 
ACCATTAGAT TAATATATTT CAAAAATAAG ATGGCAGAAG GGGATGAACT TGTGGTTCCT 
GAAACATCAG ATAATAGTCG GAAACAAATG GTTAGAAATA TCAACAACAA ACGGCGTTAC 
TCCTTCCGAG TCCCTGAAGA AGAGAGGATG CAACAGAGGA GGGAGATGCT ACGAGATCCA 
GAAATGAGAA ATAAATTAAT TTCTAACCCA ACTAATTTTA ACCACATAGC ACACATGGGT 
CCTGGAGATG GAATACAGAT CCTAAAAGAC CTGCCCATGC CAGGTTTCCC TTATTCACCC 
CCTCATCACC ACTCCGGCCT GATCTCCTCT CCGAGCAACT TTGAGCACAT CTATCACATG 
ACTGTCACTT CTGCTGAACA GTTTCTCTCT CCTGAGTCCA TAAGCCCTGG GTCCCCCCCG 
TCCCTGCGCT CTGCCCCTGG CACACCACAC TCTGTGACTC TGAGGAACCC ACGGCCTCAG 
GAAAGCCGGA CAGTATTCAG TGGCTCCGTC AGTATTCCCT CCATCACCAA GTCCCGCCCT 
GAGCCAGGCC GCTCCATGAG TGCCAGCAGC GGCCTGTCTG CACGGTCGTC AGCCCAGAAT 
GGCAGTGCAC TAAAGAGGGA ATTCTCTGGA GGAAGCTATA ACACAAAGCG ACAGCCCATG 
CCCTCTCCCT CGGAGGGCTC TTTGTCATCA GGAGGCATGG ACCAAGGAAG TGACGCCCCT 
GCGAGGGACT ATGATGGAGA GGACTCTGAT TCTCCAAGGC ATTCCACAGC TTCCAACAGC 
TCTAACCTGA GCAGCCCCCC AAGTCCCATT TCACCCCAAA AGACCAAGAG CCTCTCCCTG 
GAGAGCACAG ACCGCGGGAG CTGGGATCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109369.1
CDS	1502..6574
Translation	MKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_030253509.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253509.1

ORF Insert Sequence:

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGATG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGACTAACAG AATTGCACAC CCAAAAGCAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGCT GAGAGCTTAA GGCAGGAGCT GCGCAGAGCA 
GAAAGAGCCA AGAAAGAGCT GGAAGTTCAC ACCGAAGCTC TAATTGCTGA AGCATCAAAG 
GACAAGAAGC TCCGAGAGCA GAGCGAACAT TATTCTAAGC AACTGGAAAA CGAGTTGGAG 
GGCCTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAATAT GCAGCATAGA ACATCAGCAA 
GAGATAACTA AACTGAAAAC TGACTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAAATT 
TCTAAAAGAG AAGGAATTCA CGCAAGTGAG ATTAAAAACC TGAAGAAGGA GCTGCATGAC 
TCAGAAGGCC AGCAGCTTGC TCTCAACAAA GAGATTCTGG TTTTAAAAGA CAAATTGGAA 
AAAACCAGGA GAGAAAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAACAACAA 
TATGAACGGG AAAAAGTATT ATTAACTGAA GAGAATAAAA AGTTAACGAG TGAACTCGAT 
AAGCTGACTT CACTGTATGA GAGCTTAAGT TTGCGCAACC AGCACTTAGA AGAAGAAGTC 
AAAGATCTAG CAGATAAGAA GGAATCAGTT GCCCACTGGG AAGCCCAAAT CACAGAGATA 
ATTCAGTGGG TGAGCGATGA GAAGGATGCA CGAGGCTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AGTTGGAAGC ATTAAGAAAT TCCAGCTTGG GGACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCAAAACTG GACATGTCGG CTAGACTAGA GTTGCAGTCT 
GCTCTGGATG CAGAAATAAG AGCTAAGCAG GCCATCCAGG AAGAGCTGAA TAAAGTCAAA 
GCATCTAACA TCTTAACAGA ATGTAAACTG AAAGATTCAG AGAAGAAGAA CTTGGAACTG 
TTGTCCGAAA TCGAGCAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GACCAATTTG AACGCTCTCC GTCCTGTACT CCAGCTAGCA AAGGCAGACG TATTGCAGAC 
TGTGCTCCAC TTCCAGCTCA CACACCAACC TTAAGGAAAA AGGGATGCCC GGCTTCAACT 
GGCTTTCCAC CTAAGCGCAA GACTCATCAA TTTTTCGTGA AGTCTTTCAC TGCCCCTACC 
AAGTGTCACC AGTGCACCTC CTTGATGGTC GGTTTGATAA GACAGGGCTG TTCCTGTGAA 
GTCTGTGGGT TCTCATGTCA TATAACTTGT GTGAACAAAG CCCCGACGGT TTGCCCAGTT 
CCTCCTGAGC AAACTAAAGG TCCACTGGGC ATAGACCCCC AGAAGGGAGT TGGGACGGCG 
TATGAGGGTC ATGTCAGGAT CCCTAAGCCG GCGGGAGTGA AGAAAGGGTG GCAGAGAGCA 
CTGGCTGTGG TCTGCGACTT CAAACTGTTT CTTTATGATA TCGCAGAGGG AAAAGCATCG 
CAGCCCACCT CCGTCATTAG TCAAGTGATT GACATGAGAG ATGAAGAATT TTCTGTGAGT 
TCAGTCTTAG CTTCTGATGT TATTCACGCA AGTCGAAAAG ATATCCCCTG TATATTCAGG 
GTCACAGCTT CCCAGCTCTC AGCACCTAGT AACAAATGTT CCATCCTGAT GCTAGCAGAC 
AGCGAGAATG AGAGGAGTAA GTGGGTGGGA GTGCTGAGTG AACTCCACAA GATTCTGAAG 
AAAAACAAGT TCAGAGACCG CTCAGTGTAT GTCCCCAAGG AGGCGTACGA CAGCACGCTA 
CCCCTCATTA AGACAACGCA GGCCGCTGCA ATCATAGATC ATGAACGGAT AGCTTTGGGA 
AATGAAGAAG GATTATTTGT TGTGCATGTC ACCAAAGATG AGATTGTTAG AGTTGGTGAT 
AATAAGAAAA TCCATCAGAT CGAACTCATT CCAAGTGACC AGCTTGTCGC TGTGATCTCA 
GGCCGAAATC GCCACGTCCG ACTTTTTCCT ATGTCAGCCT TGGATGGGCG AGAGACGGAT 
TTTTATAAAC TGGCAGAGAC TAAAGGGTGT CAGACCATAG CTGCTGGGAA AGTGCGCCAC 
GGAGCTCTGT CCTGCCTGTG TGTGGCCATG AAGAGACAGG TCCTCTGCTA TGAACTGTTC 
CAAAGCAAGA CCAGGCACAG GAAGTTTAAG GAGATTCAAG TCCCATGTAA TGTCCAGTGG 
ATGGCCATCT TCAGTGAGCA TCTCTGTGTG GGATTCCAGT CAGGATTTCT CAGATACCCC 
CTGAATGGAG AAGGAGGTCC ATGCAATATG CTCCATTCAA ATGACCATAC ACTGTCATTC 
ATTTCACACC AACCAATGGA CGCTCTCTGT GCAGTTGAGA TCTCCAACAA AGAGTACTTG 
CTGTGTTTTA ACAGCATTGG GATATACACA GACTGCCAGG GCCGAAGATC CAGACAGCAG 
GAACTGATGT GGCCAGCAAA TCCTTCCTCC TGTTGTTACA ATGCTCCATA TCTCTCCGTG 
TATAGTGAAA ACGCAGTTGA TATCTTTGAT GTAAACTCCA TGGAATGGAT ACAGACTCTT 
CCTCTCAAAA AGGTCCGACC TTTAAACACT GAAGGATCAT TAAATCTCTT AGGATTGGAG 
ACCATTAGAT TAATATATTT CAAAAATAAG ATGGCAGAAG GGGATGAACT TGTGGTTCCT 
GAAACATCAG ATAATAGTCG GAAACAAATG GTTAGAAATA TCAACAACAA ACGGCGTTAC 
TCCTTCCGAG TCCCTGAAGA AGAGAGGATG CAACAGAGGA GGGAGATGCT ACGAGATCCA 
GAAATGAGAA ATAAATTAAT TTCTAACCCA ACTAATTTTA ACCACATAGC ACACATGGGT 
CCTGGAGATG GAATACAGAT CCTAAAAGAC CTGCCCATGC CAGGTTTCCC TTATTCACCC 
CCTCATCACC ACTCCGGCCT GATCTCCTCT CCGAGCAACT TTGAGCACAT CTATCACATG 
ACTGTCACTT CTGCTGAACA GTTTCTCTCT CCTGAGTCCA TAAGCCCTGG GTCCCCCCCG 
TCCCTGCGCT CTGCCCCTGG CACACCACAC TCTGTGACTC TGAGGAACCC ACGGCCTCAG 
GAAAGCCGGA CAGTATTCAG TGGCTCCGTC AGTATTCCCT CCATCACCAA GTCCCGCCCT 
GAGCCAGGCC GCTCCATGAG TGCCAGCAGC GGCCTGTCTG CACGGTCGTC AGCCCAGAAT 
GGCAGTGCAC TAAAGAGGGA ATTCTCTGGA GGAAGCTATA ACACAAAGCG ACAGCCCATG 
CCCTCTCCCT CGGAGGGCTC TTTGTCATCA GGAGGCATGG ACCAAGGAAG TGACGCCCCT 
GCGAGGGACT ATGATGGAGA GGACTCTGAT TCTCCAAGGC ATTCCACAGC TTCCAACAGC 
TCTAACCTGA GCAGCCCCCC AAGTCCCATT TCACCCCAAA AGACCAAGAG CCTCTCCCTG 
GAGAGCACAG ACCGCGGGAG CTGGGATCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_030253510.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109370.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X13, mRNA.

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CloneID	OMu85131
Clone ID Related Accession (Same CDS sequence)	XM_030253510.1
Accession Version	XM_030253510.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGATG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGACTAACAG AATTGCACAC CCAAAAGCAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGCT GAGAGCTTAA GGCAGGAGCT GCGCAGAGCA 
GAAAGAGCCA AGAAAGAGCT GGAAGTTCAC ACCGAAGCTC TAATTGCTGA AGCATCAAAG 
GACAAGAAGC TCCGAGAGCA GAGCGAACAT TATTCTAAGC AACTGGAAAA CGAGTTGGAG 
GGCCTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAATAT GCAGCATAGA ACATCAGCAA 
GAGATAACTA AACTGAAAAC TGACTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAAATT 
TCTAAAAGAG AAGGAATTCA CGCAAGTGAG ATTAAAAACC TGAAGAAGGA GCTGCATGAC 
TCAGAAGGCC AGCAGCTTGC TCTCAACAAA GAGATTCTGG TTTTAAAAGA CAAATTGGAA 
AAAACCAGGA GAGAAAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAACAACAA 
TATGAACGGG AAAAAGTATT ATTAACTGAA GAGAATAAAA AGTTAACGAG TGAACTCGAT 
AAGCTGACTT CACTGTATGA GAGCTTAAGT TTGCGCAACC AGCACTTAGA AGAAGAAGTC 
AAAGATCTAG CAGATAAGAA GGAATCAGTT GCCCACTGGG AAGCCCAAAT CACAGAGATA 
ATTCAGTGGG TGAGCGATGA GAAGGATGCA CGAGGCTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AGTTGGAAGC ATTAAGAAAT TCCAGCTTGG GGACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCAAAACTG GACATGTCGG CTAGACTAGA GTTGCAGTCT 
GCTCTGGATG CAGAAATAAG AGCTAAGCAG GCCATCCAGG AAGAGCTGAA TAAAGTCAAA 
GCATCTAACA TCTTAACAGA ATGTAAACTG AAAGATTCAG AGAAGAAGAA CTTGGAACTG 
TTGTCCGAAA TCGAGCAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GACCAATTTG AAATTGCAGA CTGTGCTCCA CTTCCAGCTC ACACACCAAC CTTAAGGAAA 
AAGGGATGCC CGGCTTCAAC TGGCTTTCCA CCTAAGCGCA AGACTCATCA ATTTTTCGTG 
AAGTCTTTCA CTGCCCCTAC CAAGTGTCAC CAGTGCACCT CCTTGATGGT CGGTTTGATA 
AGACAGGGCT GTTCCTGTGA AGTCTGTGGG TTCTCATGTC ATATAACTTG TGTGAACAAA 
GCCCCGACGG TTTGCCCAGT TCCTCCTGAG CAAACTAAAG GTCCACTGGG CATAGACCCC 
CAGAAGGGAG TTGGGACGGC GTATGAGGGT CATGTCAGGA TCCCTAAGCC GGCGGGAGTG 
AAGAAAGGGT GGCAGAGAGC ACTGGCTGTG GTCTGCGACT TCAAACTGTT TCTTTATGAT 
ATCGCAGAGG GAAAAGCATC GCAGCCCACC TCCGTCATTA GTCAAGTGAT TGACATGAGA 
GATGAAGAAT TTTCTGTGAG TTCAGTCTTA GCTTCTGATG TTATTCACGC AAGTCGAAAA 
GATATCCCCT GTATATTCAG GGTCACAGCT TCCCAGCTCT CAGCACCTAG TAACAAATGT 
TCCATCCTGA TGCTAGCAGA CAGCGAGAAT GAGAGGAGTA AGTGGGTGGG AGTGCTGAGT 
GAACTCCACA AGATTCTGAA GAAAAACAAG TTCAGAGACC GCTCAGTGTA TGTCCCCAAG 
GAGGCGTACG ACAGCACGCT ACCCCTCATT AAGACAACGC AGGCCGCTGC AATCATAGAT 
CATGAACGGA TAGCTTTGGG AAATGAAGAA GGATTATTTG TTGTGCATGT CACCAAAGAT 
GAGATTGTTA GAGTTGGTGA TAATAAGAAA ATCCATCAGA TCGAACTCAT TCCAAGTGAC 
CAGCTTGTCG CTGTGATCTC AGGCCGAAAT CGCCACGTCC GACTTTTTCC TATGTCAGCC 
TTGGATGGGC GAGAGACGGA TTTTTATAAA CTGGCAGAGA CTAAAGGGTG TCAGACCATA 
GCTGCTGGGA AAGTGCGCCA CGGAGCTCTG TCCTGCCTGT GTGTGGCCAT GAAGAGACAG 
GTCCTCTGCT ATGAACTGTT CCAAAGCAAG ACCAGGCACA GGAAGTTTAA GGAGATTCAA 
GTCCCATGTA ATGTCCAGTG GATGGCCATC TTCAGTGAGC ATCTCTGTGT GGGATTCCAG 
TCAGGATTTC TCAGATACCC CCTGAATGGA GAAGGAGGTC CATGCAATAT GCTCCATTCA 
AATGACCATA CACTGTCATT CATTTCACAC CAACCAATGG ACGCTCTCTG TGCAGTTGAG 
ATCTCCAACA AAGAGTACTT GCTGTGTTTT AACAGCATTG GGATATACAC AGACTGCCAG 
GGCCGAAGAT CCAGACAGCA GGAACTGATG TGGCCAGCAA ATCCTTCCTC CTGTTGTTAC 
AATGCTCCAT ATCTCTCCGT GTATAGTGAA AACGCAGTTG ATATCTTTGA TGTAAACTCC 
ATGGAATGGA TACAGACTCT TCCTCTCAAA AAGGTCCGAC CTTTAAACAC TGAAGGATCA 
TTAAATCTCT TAGGATTGGA GACCATTAGA TTAATATATT TCAAAAATAA GATGGCAGAA 
GGGGATGAAC TTGTGGTTCC TGAAACATCA GATAATAGTC GGAAACAAAT GGTTAGAAAT 
ATCAACAACA AACGGCGTTA CTCCTTCCGA GTCCCTGAAG AAGAGAGGAT GCAACAGAGG 
AGGGAGATGC TACGAGATCC AGAAATGAGA AATAAATTAA TTTCTAACCC AACTAATTTT 
AACCACATAG CACACATGGG TCCTGGAGAT GGAATACAGA TCCTAAAAGA CCTGCCCATG 
CCAGGTTTCC CTTATTCACC CCCTCATCAC CACTCCGGCC TGATCTCCTC TCCGAGCAAC 
TTTGAGCACA TCTATCACAT GACTGTCACT TCTGCTGAAC AGTTTCTCTC TCCTGAGTCC 
ATAAGCCCTG GGTCCCCCCC GTCCCTGCGC TCTGCCCCTG GCACACCACA CTCTGTGACT 
CTGAGGAACC CACGGCCTCA GGAAAGCCGG ACAGTATTCA GTGGCTCCGT CAGTATTCCC 
TCCATCACCA AGTCCCGCCC TGAGCCAGGC CGCTCCATGA GTGCCAGCAG CGGCCTGTCT 
GCACGGTCGT CAGCCCAGAA TGGCAGTGCA CTAAAGAGGG AATTCTCTGG AGGAAGCTAT 
AACACAAAGC GACAGCCCAT GCCCTCTCCC TCGGAGGGCT CTTTGTCATC AGGAGGCATG 
GACCAAGGAA GTGACGCCCC TGCGAGGGAC TATGATGGAG AGGACTCTGA TTCTCCAAGG 
CATTCCACAG CTTCCAACAG CTCTAACCTG AGCAGCCCCC CAAGTCCCAT TTCACCCCAA 
AAGACCAAGA GCCTCTCCCT GGAGAGCACA GACCGCGGGA GCTGGGATCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109370.1
CDS	1203..6236
Translation	MKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_030253510.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253510.1

ORF Insert Sequence:

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGATG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGACTAACAG AATTGCACAC CCAAAAGCAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGCT GAGAGCTTAA GGCAGGAGCT GCGCAGAGCA 
GAAAGAGCCA AGAAAGAGCT GGAAGTTCAC ACCGAAGCTC TAATTGCTGA AGCATCAAAG 
GACAAGAAGC TCCGAGAGCA GAGCGAACAT TATTCTAAGC AACTGGAAAA CGAGTTGGAG 
GGCCTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAATAT GCAGCATAGA ACATCAGCAA 
GAGATAACTA AACTGAAAAC TGACTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAAATT 
TCTAAAAGAG AAGGAATTCA CGCAAGTGAG ATTAAAAACC TGAAGAAGGA GCTGCATGAC 
TCAGAAGGCC AGCAGCTTGC TCTCAACAAA GAGATTCTGG TTTTAAAAGA CAAATTGGAA 
AAAACCAGGA GAGAAAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAACAACAA 
TATGAACGGG AAAAAGTATT ATTAACTGAA GAGAATAAAA AGTTAACGAG TGAACTCGAT 
AAGCTGACTT CACTGTATGA GAGCTTAAGT TTGCGCAACC AGCACTTAGA AGAAGAAGTC 
AAAGATCTAG CAGATAAGAA GGAATCAGTT GCCCACTGGG AAGCCCAAAT CACAGAGATA 
ATTCAGTGGG TGAGCGATGA GAAGGATGCA CGAGGCTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AGTTGGAAGC ATTAAGAAAT TCCAGCTTGG GGACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCAAAACTG GACATGTCGG CTAGACTAGA GTTGCAGTCT 
GCTCTGGATG CAGAAATAAG AGCTAAGCAG GCCATCCAGG AAGAGCTGAA TAAAGTCAAA 
GCATCTAACA TCTTAACAGA ATGTAAACTG AAAGATTCAG AGAAGAAGAA CTTGGAACTG 
TTGTCCGAAA TCGAGCAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GACCAATTTG AAATTGCAGA CTGTGCTCCA CTTCCAGCTC ACACACCAAC CTTAAGGAAA 
AAGGGATGCC CGGCTTCAAC TGGCTTTCCA CCTAAGCGCA AGACTCATCA ATTTTTCGTG 
AAGTCTTTCA CTGCCCCTAC CAAGTGTCAC CAGTGCACCT CCTTGATGGT CGGTTTGATA 
AGACAGGGCT GTTCCTGTGA AGTCTGTGGG TTCTCATGTC ATATAACTTG TGTGAACAAA 
GCCCCGACGG TTTGCCCAGT TCCTCCTGAG CAAACTAAAG GTCCACTGGG CATAGACCCC 
CAGAAGGGAG TTGGGACGGC GTATGAGGGT CATGTCAGGA TCCCTAAGCC GGCGGGAGTG 
AAGAAAGGGT GGCAGAGAGC ACTGGCTGTG GTCTGCGACT TCAAACTGTT TCTTTATGAT 
ATCGCAGAGG GAAAAGCATC GCAGCCCACC TCCGTCATTA GTCAAGTGAT TGACATGAGA 
GATGAAGAAT TTTCTGTGAG TTCAGTCTTA GCTTCTGATG TTATTCACGC AAGTCGAAAA 
GATATCCCCT GTATATTCAG GGTCACAGCT TCCCAGCTCT CAGCACCTAG TAACAAATGT 
TCCATCCTGA TGCTAGCAGA CAGCGAGAAT GAGAGGAGTA AGTGGGTGGG AGTGCTGAGT 
GAACTCCACA AGATTCTGAA GAAAAACAAG TTCAGAGACC GCTCAGTGTA TGTCCCCAAG 
GAGGCGTACG ACAGCACGCT ACCCCTCATT AAGACAACGC AGGCCGCTGC AATCATAGAT 
CATGAACGGA TAGCTTTGGG AAATGAAGAA GGATTATTTG TTGTGCATGT CACCAAAGAT 
GAGATTGTTA GAGTTGGTGA TAATAAGAAA ATCCATCAGA TCGAACTCAT TCCAAGTGAC 
CAGCTTGTCG CTGTGATCTC AGGCCGAAAT CGCCACGTCC GACTTTTTCC TATGTCAGCC 
TTGGATGGGC GAGAGACGGA TTTTTATAAA CTGGCAGAGA CTAAAGGGTG TCAGACCATA 
GCTGCTGGGA AAGTGCGCCA CGGAGCTCTG TCCTGCCTGT GTGTGGCCAT GAAGAGACAG 
GTCCTCTGCT ATGAACTGTT CCAAAGCAAG ACCAGGCACA GGAAGTTTAA GGAGATTCAA 
GTCCCATGTA ATGTCCAGTG GATGGCCATC TTCAGTGAGC ATCTCTGTGT GGGATTCCAG 
TCAGGATTTC TCAGATACCC CCTGAATGGA GAAGGAGGTC CATGCAATAT GCTCCATTCA 
AATGACCATA CACTGTCATT CATTTCACAC CAACCAATGG ACGCTCTCTG TGCAGTTGAG 
ATCTCCAACA AAGAGTACTT GCTGTGTTTT AACAGCATTG GGATATACAC AGACTGCCAG 
GGCCGAAGAT CCAGACAGCA GGAACTGATG TGGCCAGCAA ATCCTTCCTC CTGTTGTTAC 
AATGCTCCAT ATCTCTCCGT GTATAGTGAA AACGCAGTTG ATATCTTTGA TGTAAACTCC 
ATGGAATGGA TACAGACTCT TCCTCTCAAA AAGGTCCGAC CTTTAAACAC TGAAGGATCA 
TTAAATCTCT TAGGATTGGA GACCATTAGA TTAATATATT TCAAAAATAA GATGGCAGAA 
GGGGATGAAC TTGTGGTTCC TGAAACATCA GATAATAGTC GGAAACAAAT GGTTAGAAAT 
ATCAACAACA AACGGCGTTA CTCCTTCCGA GTCCCTGAAG AAGAGAGGAT GCAACAGAGG 
AGGGAGATGC TACGAGATCC AGAAATGAGA AATAAATTAA TTTCTAACCC AACTAATTTT 
AACCACATAG CACACATGGG TCCTGGAGAT GGAATACAGA TCCTAAAAGA CCTGCCCATG 
CCAGGTTTCC CTTATTCACC CCCTCATCAC CACTCCGGCC TGATCTCCTC TCCGAGCAAC 
TTTGAGCACA TCTATCACAT GACTGTCACT TCTGCTGAAC AGTTTCTCTC TCCTGAGTCC 
ATAAGCCCTG GGTCCCCCCC GTCCCTGCGC TCTGCCCCTG GCACACCACA CTCTGTGACT 
CTGAGGAACC CACGGCCTCA GGAAAGCCGG ACAGTATTCA GTGGCTCCGT CAGTATTCCC 
TCCATCACCA AGTCCCGCCC TGAGCCAGGC CGCTCCATGA GTGCCAGCAG CGGCCTGTCT 
GCACGGTCGT CAGCCCAGAA TGGCAGTGCA CTAAAGAGGG AATTCTCTGG AGGAAGCTAT 
AACACAAAGC GACAGCCCAT GCCCTCTCCC TCGGAGGGCT CTTTGTCATC AGGAGGCATG 
GACCAAGGAA GTGACGCCCC TGCGAGGGAC TATGATGGAG AGGACTCTGA TTCTCCAAGG 
CATTCCACAG CTTCCAACAG CTCTAACCTG AGCAGCCCCC CAAGTCCCAT TTCACCCCAA 
AAGACCAAGA GCCTCTCCCT GGAGAGCACA GACCGCGGGA GCTGGGATCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( NM_001033285.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001028457.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu12565
Clone ID Related Accession (Same CDS sequence)	NM_001033285.1
Accession Version	NM_001033285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC125380.4 and AC132436.3. On or before Jul 26, 2009 this sequence version replaced XM_976135.1, XM_976025.1, XM_975992.1, XM_976101.1, XM_975852.1, XM_975917.1, XM_991276.1, XM_991198.1, XM_991226.1, XM_991179.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC158017.1, SRR1660817.67078.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAACGCTCT CCGTCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTATTGCAG ACTGTGCTCC ACTTCCAGCT CACACACCAA CCTTAAGGAA AAAGGGATGC 
CCGGCTTCAA CTGGCTTTCC ACCTAAGCGC AAGACTCATC AATTTTTCGT GAAGTCTTTC 
ACTGCCCCTA CCAAGTGTCA CCAGTGCACC TCCTTGATGG TCGGTTTGAT AAGACAGGGC 
TGTTCCTGTG AAGTCTGTGG GTTCTCATGT CATATAACTT GTGTGAACAA AGCCCCGACG 
GTTTGCCCAG TTCCTCCTGA GCAAACTAAA GGTCCACTGG GCATAGACCC CCAGAAGGGA 
GTTGGGACGG CGTATGAGGG TCATGTCAGG ATCCCTAAGC CGGCGGGAGT GAAGAAAGGG 
TGGCAGAGAG CACTGGCTGT GGTCTGCGAC TTCAAACTGT TTCTTTATGA TATCGCAGAG 
GGAAAAGCAT CGCAGCCCAC CTCCGTCATT AGTCAAGTGA TTGACATGAG AGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT AGCTTCTGAT GTTATTCACG CAAGTCGAAA AGATATCCCC 
TGTATATTCA GGGTCACAGC TTCCCAGCTC TCAGCACCTA GTAACAAATG TTCCATCCTG 
ATGCTAGCAG ACAGCGAGAA TGAGAGGAGT AAGTGGGTGG GAGTGCTGAG TGAACTCCAC 
AAGATTCTGA AGAAAAACAA GTTCAGAGAC CGCTCAGTGT ATGTCCCCAA GGAGGCGTAC 
GACAGCACGC TACCCCTCAT TAAGACAACG CAGGCCGCTG CAATCATAGA TCATGAACGG 
ATAGCTTTGG GAAATGAAGA AGGATTATTT GTTGTGCATG TCACCAAAGA TGAGATTGTT 
AGAGTTGGTG ATAATAAGAA AATCCATCAG ATCGAACTCA TTCCAAGTGA CCAGCTTGTC 
GCTGTGATCT CAGGCCGAAA TCGCCACGTC CGACTTTTTC CTATGTCAGC CTTGGATGGG 
CGAGAGACGG ATTTTTATAA ACTGGCAGAG ACTAAAGGGT GTCAGACCAT AGCTGCTGGG 
AAAGTGCGCC ACGGAGCTCT GTCCTGCCTG TGTGTGGCCA TGAAGAGACA GGTCCTCTGC 
TATGAACTGT TCCAAAGCAA GACCAGGCAC AGGAAGTTTA AGGAGATTCA AGTCCCATGT 
AATGTCCAGT GGATGGCCAT CTTCAGTGAG CATCTCTGTG TGGGATTCCA GTCAGGATTT 
CTCAGATACC CCCTGAATGG AGAAGGAGGT CCATGCAATA TGCTCCATTC AAATGACCAT 
ACACTGTCAT TCATTTCACA CCAACCAATG GACGCTCTCT GTGCAGTTGA GATCTCCAAC 
AAAGAGTACT TGCTGTGTTT TAACAGCATT GGGATATACA CAGACTGCCA GGGCCGAAGA 
TCCAGACAGC AGGAACTGAT GTGGCCAGCA AATCCTTCCT CCTGTTGTTA CAATGCTCCA 
TATCTCTCCG TGTATAGTGA AAACGCAGTT GATATCTTTG ATGTAAACTC CATGGAATGG 
ATACAGACTC TTCCTCTCAA AAAGGTCCGA CCTTTAAACA CTGAAGGATC ATTAAATCTC 
TTAGGATTGG AGACCATTAG ATTAATATAT TTCAAAAATA AGATGGCAGA AGGGGATGAA 
CTTGTGGTTC CTGAAACATC AGATAATAGT CGGAAACAAA TGGTTAGAAA TATCAACAAC 
AAACGGCGTT ACTCCTTCCG AGTCCCTGAA GAAGAGAGGA TGCAACAGAG GAGGGAGATG 
CTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAACC CAACTAATTT TAACCACATA 
GCACACATGG GTCCTGGAGA TGGAATACAG ATCCTAAAAG ACCTGCCCAT GAACCCACGG 
CCTCAGGAAA GCCGGACAGT ATTCAGTGGC TCCGTCAGTA TTCCCTCCAT CACCAAGTCC 
CGCCCTGAGC CAGGCCGCTC CATGAGTGCC AGCAGCGGCC TGTCTGCACG GTCGTCAGCC 
CAGAATGGCA GTGCACTAAA GAGGGAATTC TCTGGAGGAA GCTATAACAC AAAGCGACAG 
CCCATGCCCT CTCCCTCGGA GGGCTCTTTG TCATCAGGAG GCATGGACCA AGGAAGTGAC 
GCCCCTGCGA GGGACTATGA TGGAGAGGAC TCTGATTCTC CAAGGCATTC CACAGCTTCC 
AACAGCTCTA ACCTGAGCAG CCCCCCAAGT CCCATTTCAC CCCAAAAGAC CAAGAGCCTC 
TCCCTGGAGA GCACAGACCG CGGGAGCTGG GATCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028457.1
CDS	22..5220
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	NM_001033285.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033285.1

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAACGCTCT CCGTCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTATTGCAG ACTGTGCTCC ACTTCCAGCT CACACACCAA CCTTAAGGAA AAAGGGATGC 
CCGGCTTCAA CTGGCTTTCC ACCTAAGCGC AAGACTCATC AATTTTTCGT GAAGTCTTTC 
ACTGCCCCTA CCAAGTGTCA CCAGTGCACC TCCTTGATGG TCGGTTTGAT AAGACAGGGC 
TGTTCCTGTG AAGTCTGTGG GTTCTCATGT CATATAACTT GTGTGAACAA AGCCCCGACG 
GTTTGCCCAG TTCCTCCTGA GCAAACTAAA GGTCCACTGG GCATAGACCC CCAGAAGGGA 
GTTGGGACGG CGTATGAGGG TCATGTCAGG ATCCCTAAGC CGGCGGGAGT GAAGAAAGGG 
TGGCAGAGAG CACTGGCTGT GGTCTGCGAC TTCAAACTGT TTCTTTATGA TATCGCAGAG 
GGAAAAGCAT CGCAGCCCAC CTCCGTCATT AGTCAAGTGA TTGACATGAG AGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT AGCTTCTGAT GTTATTCACG CAAGTCGAAA AGATATCCCC 
TGTATATTCA GGGTCACAGC TTCCCAGCTC TCAGCACCTA GTAACAAATG TTCCATCCTG 
ATGCTAGCAG ACAGCGAGAA TGAGAGGAGT AAGTGGGTGG GAGTGCTGAG TGAACTCCAC 
AAGATTCTGA AGAAAAACAA GTTCAGAGAC CGCTCAGTGT ATGTCCCCAA GGAGGCGTAC 
GACAGCACGC TACCCCTCAT TAAGACAACG CAGGCCGCTG CAATCATAGA TCATGAACGG 
ATAGCTTTGG GAAATGAAGA AGGATTATTT GTTGTGCATG TCACCAAAGA TGAGATTGTT 
AGAGTTGGTG ATAATAAGAA AATCCATCAG ATCGAACTCA TTCCAAGTGA CCAGCTTGTC 
GCTGTGATCT CAGGCCGAAA TCGCCACGTC CGACTTTTTC CTATGTCAGC CTTGGATGGG 
CGAGAGACGG ATTTTTATAA ACTGGCAGAG ACTAAAGGGT GTCAGACCAT AGCTGCTGGG 
AAAGTGCGCC ACGGAGCTCT GTCCTGCCTG TGTGTGGCCA TGAAGAGACA GGTCCTCTGC 
TATGAACTGT TCCAAAGCAA GACCAGGCAC AGGAAGTTTA AGGAGATTCA AGTCCCATGT 
AATGTCCAGT GGATGGCCAT CTTCAGTGAG CATCTCTGTG TGGGATTCCA GTCAGGATTT 
CTCAGATACC CCCTGAATGG AGAAGGAGGT CCATGCAATA TGCTCCATTC AAATGACCAT 
ACACTGTCAT TCATTTCACA CCAACCAATG GACGCTCTCT GTGCAGTTGA GATCTCCAAC 
AAAGAGTACT TGCTGTGTTT TAACAGCATT GGGATATACA CAGACTGCCA GGGCCGAAGA 
TCCAGACAGC AGGAACTGAT GTGGCCAGCA AATCCTTCCT CCTGTTGTTA CAATGCTCCA 
TATCTCTCCG TGTATAGTGA AAACGCAGTT GATATCTTTG ATGTAAACTC CATGGAATGG 
ATACAGACTC TTCCTCTCAA AAAGGTCCGA CCTTTAAACA CTGAAGGATC ATTAAATCTC 
TTAGGATTGG AGACCATTAG ATTAATATAT TTCAAAAATA AGATGGCAGA AGGGGATGAA 
CTTGTGGTTC CTGAAACATC AGATAATAGT CGGAAACAAA TGGTTAGAAA TATCAACAAC 
AAACGGCGTT ACTCCTTCCG AGTCCCTGAA GAAGAGAGGA TGCAACAGAG GAGGGAGATG 
CTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAACC CAACTAATTT TAACCACATA 
GCACACATGG GTCCTGGAGA TGGAATACAG ATCCTAAAAG ACCTGCCCAT GAACCCACGG 
CCTCAGGAAA GCCGGACAGT ATTCAGTGGC TCCGTCAGTA TTCCCTCCAT CACCAAGTCC 
CGCCCTGAGC CAGGCCGCTC CATGAGTGCC AGCAGCGGCC TGTCTGCACG GTCGTCAGCC 
CAGAATGGCA GTGCACTAAA GAGGGAATTC TCTGGAGGAA GCTATAACAC AAAGCGACAG 
CCCATGCCCT CTCCCTCGGA GGGCTCTTTG TCATCAGGAG GCATGGACCA AGGAAGTGAC 
GCCCCTGCGA GGGACTATGA TGGAGAGGAC TCTGATTCTC CAAGGCATTC CACAGCTTCC 
AACAGCTCTA ACCTGAGCAG CCCCCCAAGT CCCATTTCAC CCCAAAAGAC CAAGAGCCTC 
TCCCTGGAGA GCACAGACCG CGGGAGCTGG GATCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( NM_001359541.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346470.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 4, mRNA.

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CloneID	OMu28725
Clone ID Related Accession (Same CDS sequence)	NM_001359541.1 , XM_006496805.3
Accession Version	NM_001359541.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC125380.4 and AC132436.3. On Jan 13, 2018 this sequence version replaced XM_006496805.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.66083.1, SRR1660815.124225.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAAATTGCA GACTGTGCTC CACTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG CCCGGCTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAT 
CAATTTTTCG TGAAGTCTTT CACTGCCCCT ACCAAGTGTC ACCAGTGCAC CTCCTTGATG 
GTCGGTTTGA TAAGACAGGG CTGTTCCTGT GAAGTCTGTG GGTTCTCATG TCATATAACT 
TGTGTGAACA AAGCCCCGAC GGTTTGCCCA GTTCCTCCTG AGCAAACTAA AGGTCCACTG 
GGCATAGACC CCCAGAAGGG AGTTGGGACG GCGTATGAGG GTCATGTCAG GATCCCTAAG 
CCGGCGGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTG TGGTCTGCGA CTTCAAACTG 
TTTCTTTATG ATATCGCAGA GGGAAAAGCA TCGCAGCCCA CCTCCGTCAT TAGTCAAGTG 
ATTGACATGA GAGATGAAGA ATTTTCTGTG AGTTCAGTCT TAGCTTCTGA TGTTATTCAC 
GCAAGTCGAA AAGATATCCC CTGTATATTC AGGGTCACAG CTTCCCAGCT CTCAGCACCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCA GACAGCGAGA ATGAGAGGAG TAAGTGGGTG 
GGAGTGCTGA GTGAACTCCA CAAGATTCTG AAGAAAAACA AGTTCAGAGA CCGCTCAGTG 
TATGTCCCCA AGGAGGCGTA CGACAGCACG CTACCCCTCA TTAAGACAAC GCAGGCCGCT 
GCAATCATAG ATCATGAACG GATAGCTTTG GGAAATGAAG AAGGATTATT TGTTGTGCAT 
GTCACCAAAG ATGAGATTGT TAGAGTTGGT GATAATAAGA AAATCCATCA GATCGAACTC 
ATTCCAAGTG ACCAGCTTGT CGCTGTGATC TCAGGCCGAA ATCGCCACGT CCGACTTTTT 
CCTATGTCAG CCTTGGATGG GCGAGAGACG GATTTTTATA AACTGGCAGA GACTAAAGGG 
TGTCAGACCA TAGCTGCTGG GAAAGTGCGC CACGGAGCTC TGTCCTGCCT GTGTGTGGCC 
ATGAAGAGAC AGGTCCTCTG CTATGAACTG TTCCAAAGCA AGACCAGGCA CAGGAAGTTT 
AAGGAGATTC AAGTCCCATG TAATGTCCAG TGGATGGCCA TCTTCAGTGA GCATCTCTGT 
GTGGGATTCC AGTCAGGATT TCTCAGATAC CCCCTGAATG GAGAAGGAGG TCCATGCAAT 
ATGCTCCATT CAAATGACCA TACACTGTCA TTCATTTCAC ACCAACCAAT GGACGCTCTC 
TGTGCAGTTG AGATCTCCAA CAAAGAGTAC TTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACAGACTGCC AGGGCCGAAG ATCCAGACAG CAGGAACTGA TGTGGCCAGC AAATCCTTCC 
TCCTGTTGTT ACAATGCTCC ATATCTCTCC GTGTATAGTG AAAACGCAGT TGATATCTTT 
GATGTAAACT CCATGGAATG GATACAGACT CTTCCTCTCA AAAAGGTCCG ACCTTTAAAC 
ACTGAAGGAT CATTAAATCT CTTAGGATTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTTGTGGTT CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ATATCAACAA CAAACGGCGT TACTCCTTCC GAGTCCCTGA AGAAGAGAGG 
ATGCAACAGA GGAGGGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGCCAGGTTT CCCTTATTCA CCCCCTCATC ACCACTCCGG CCTGATCTCC 
TCTCCGAGCA ACTTTGAGCA CATCTATCAC ATGACTGTCA CTTCTGCTGA ACAGTTTCTC 
TCTCCTGAGT CCATAAGCCC TGGGTCCCCC CCGTCCCTGC GCTCTGCCCC TGGCACACCA 
CACTCTGTGA CTCTGAGGAA CCCACGGCCT CAGGAAAGCC GGACAGTATT CAGTGGCTCC 
GTCAGTATTC CCTCCATCAC CAAGTCCCGC CCTGAGCCAG GCCGCTCCAT GAGTGCCAGC 
AGCGGCCTGT CTGCACGGTC GTCAGCCCAG AATGGCAGTG CACTAAAGAG GGAATTCTCT 
GGAGGAAGCT ATAACACAAA GCGACAGCCC ATGCCCTCTC CCTCGGAGGG CTCTTTGTCA 
TCAGGAGGCA TGGACCAAGG AAGTGACGCC CCTGCGAGGG ACTATGATGG AGAGGACTCT 
GATTCTCCAA GGCATTCCAC AGCTTCCAAC AGCTCTAACC TGAGCAGCCC CCCAAGTCCC 
ATTTCACCCC AAAAGACCAA GAGCCTCTCC CTGGAGAGCA CAGACCGCGG GAGCTGGGAT 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346470.1
CDS	351..5696
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	NM_001359541.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359541.1

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAAATTGCA GACTGTGCTC CACTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG CCCGGCTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAT 
CAATTTTTCG TGAAGTCTTT CACTGCCCCT ACCAAGTGTC ACCAGTGCAC CTCCTTGATG 
GTCGGTTTGA TAAGACAGGG CTGTTCCTGT GAAGTCTGTG GGTTCTCATG TCATATAACT 
TGTGTGAACA AAGCCCCGAC GGTTTGCCCA GTTCCTCCTG AGCAAACTAA AGGTCCACTG 
GGCATAGACC CCCAGAAGGG AGTTGGGACG GCGTATGAGG GTCATGTCAG GATCCCTAAG 
CCGGCGGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTG TGGTCTGCGA CTTCAAACTG 
TTTCTTTATG ATATCGCAGA GGGAAAAGCA TCGCAGCCCA CCTCCGTCAT TAGTCAAGTG 
ATTGACATGA GAGATGAAGA ATTTTCTGTG AGTTCAGTCT TAGCTTCTGA TGTTATTCAC 
GCAAGTCGAA AAGATATCCC CTGTATATTC AGGGTCACAG CTTCCCAGCT CTCAGCACCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCA GACAGCGAGA ATGAGAGGAG TAAGTGGGTG 
GGAGTGCTGA GTGAACTCCA CAAGATTCTG AAGAAAAACA AGTTCAGAGA CCGCTCAGTG 
TATGTCCCCA AGGAGGCGTA CGACAGCACG CTACCCCTCA TTAAGACAAC GCAGGCCGCT 
GCAATCATAG ATCATGAACG GATAGCTTTG GGAAATGAAG AAGGATTATT TGTTGTGCAT 
GTCACCAAAG ATGAGATTGT TAGAGTTGGT GATAATAAGA AAATCCATCA GATCGAACTC 
ATTCCAAGTG ACCAGCTTGT CGCTGTGATC TCAGGCCGAA ATCGCCACGT CCGACTTTTT 
CCTATGTCAG CCTTGGATGG GCGAGAGACG GATTTTTATA AACTGGCAGA GACTAAAGGG 
TGTCAGACCA TAGCTGCTGG GAAAGTGCGC CACGGAGCTC TGTCCTGCCT GTGTGTGGCC 
ATGAAGAGAC AGGTCCTCTG CTATGAACTG TTCCAAAGCA AGACCAGGCA CAGGAAGTTT 
AAGGAGATTC AAGTCCCATG TAATGTCCAG TGGATGGCCA TCTTCAGTGA GCATCTCTGT 
GTGGGATTCC AGTCAGGATT TCTCAGATAC CCCCTGAATG GAGAAGGAGG TCCATGCAAT 
ATGCTCCATT CAAATGACCA TACACTGTCA TTCATTTCAC ACCAACCAAT GGACGCTCTC 
TGTGCAGTTG AGATCTCCAA CAAAGAGTAC TTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACAGACTGCC AGGGCCGAAG ATCCAGACAG CAGGAACTGA TGTGGCCAGC AAATCCTTCC 
TCCTGTTGTT ACAATGCTCC ATATCTCTCC GTGTATAGTG AAAACGCAGT TGATATCTTT 
GATGTAAACT CCATGGAATG GATACAGACT CTTCCTCTCA AAAAGGTCCG ACCTTTAAAC 
ACTGAAGGAT CATTAAATCT CTTAGGATTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTTGTGGTT CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ATATCAACAA CAAACGGCGT TACTCCTTCC GAGTCCCTGA AGAAGAGAGG 
ATGCAACAGA GGAGGGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGCCAGGTTT CCCTTATTCA CCCCCTCATC ACCACTCCGG CCTGATCTCC 
TCTCCGAGCA ACTTTGAGCA CATCTATCAC ATGACTGTCA CTTCTGCTGA ACAGTTTCTC 
TCTCCTGAGT CCATAAGCCC TGGGTCCCCC CCGTCCCTGC GCTCTGCCCC TGGCACACCA 
CACTCTGTGA CTCTGAGGAA CCCACGGCCT CAGGAAAGCC GGACAGTATT CAGTGGCTCC 
GTCAGTATTC CCTCCATCAC CAAGTCCCGC CCTGAGCCAG GCCGCTCCAT GAGTGCCAGC 
AGCGGCCTGT CTGCACGGTC GTCAGCCCAG AATGGCAGTG CACTAAAGAG GGAATTCTCT 
GGAGGAAGCT ATAACACAAA GCGACAGCCC ATGCCCTCTC CCTCGGAGGG CTCTTTGTCA 
TCAGGAGGCA TGGACCAAGG AAGTGACGCC CCTGCGAGGG ACTATGATGG AGAGGACTCT 
GATTCTCCAA GGCATTCCAC AGCTTCCAAC AGCTCTAACC TGAGCAGCCC CCCAAGTCCC 
ATTTCACCCC AAAAGACCAA GAGCCTCTCC CTGGAGAGCA CAGACCGCGG GAGCTGGGAT 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( NM_001359542.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346471.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 5, mRNA.

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CloneID	OMu28728
Clone ID Related Accession (Same CDS sequence)	XM_006496808.3 , NM_001359542.1
Accession Version	NM_001359542.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC125380.4 and AC132436.3. On Jan 13, 2018 this sequence version replaced XM_006496808.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.399.1, SRR1660815.229273.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAAGATTCC CTTTCTTCTT CCTCATCCTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC GTCCTGTACT CCAGCTAGCA AAGGCAGACG TATTGCAGAC 
TGTGCTCCAC TTCCAGCTCA CACACCAACC TTAAGGAAAA AGGGATGCCC GGCTTCAACT 
GGCTTTCCAC CTAAGCGCAA GACTCATCAA TTTTTCGTGA AGTCTTTCAC TGCCCCTACC 
AAGTGTCACC AGTGCACCTC CTTGATGGTC GGTTTGATAA GACAGGGCTG TTCCTGTGAA 
GTCTGTGGGT TCTCATGTCA TATAACTTGT GTGAACAAAG CCCCGACGGT TTGCCCAGTT 
CCTCCTGAGC AAACTAAAGG TCCACTGGGC ATAGACCCCC AGAAGGGAGT TGGGACGGCG 
TATGAGGGTC ATGTCAGGAT CCCTAAGCCG GCGGGAGTGA AGAAAGGGTG GCAGAGAGCA 
CTGGCTGTGG TCTGCGACTT CAAACTGTTT CTTTATGATA TCGCAGAGGG AAAAGCATCG 
CAGCCCACCT CCGTCATTAG TCAAGTGATT GACATGAGAG ATGAAGAATT TTCTGTGAGT 
TCAGTCTTAG CTTCTGATGT TATTCACGCA AGTCGAAAAG ATATCCCCTG TATATTCAGG 
GTCACAGCTT CCCAGCTCTC AGCACCTAGT AACAAATGTT CCATCCTGAT GCTAGCAGAC 
AGCGAGAATG AGAGGAGTAA GTGGGTGGGA GTGCTGAGTG AACTCCACAA GATTCTGAAG 
AAAAACAAGT TCAGAGACCG CTCAGTGTAT GTCCCCAAGG AGGCGTACGA CAGCACGCTA 
CCCCTCATTA AGACAACGCA GGCCGCTGCA ATCATAGATC ATGAACGGAT AGCTTTGGGA 
AATGAAGAAG GATTATTTGT TGTGCATGTC ACCAAAGATG AGATTGTTAG AGTTGGTGAT 
AATAAGAAAA TCCATCAGAT CGAACTCATT CCAAGTGACC AGCTTGTCGC TGTGATCTCA 
GGCCGAAATC GCCACGTCCG ACTTTTTCCT ATGTCAGCCT TGGATGGGCG AGAGACGGAT 
TTTTATAAAC TGGCAGAGAC TAAAGGGTGT CAGACCATAG CTGCTGGGAA AGTGCGCCAC 
GGAGCTCTGT CCTGCCTGTG TGTGGCCATG AAGAGACAGG TCCTCTGCTA TGAACTGTTC 
CAAAGCAAGA CCAGGCACAG GAAGTTTAAG GAGATTCAAG TCCCATGTAA TGTCCAGTGG 
ATGGCCATCT TCAGTGAGCA TCTCTGTGTG GGATTCCAGT CAGGATTTCT CAGATACCCC 
CTGAATGGAG AAGGAGGTCC ATGCAATATG CTCCATTCAA ATGACCATAC ACTGTCATTC 
ATTTCACACC AACCAATGGA CGCTCTCTGT GCAGTTGAGA TCTCCAACAA AGAGTACTTG 
CTGTGTTTTA ACAGCATTGG GATATACACA GACTGCCAGG GCCGAAGATC CAGACAGCAG 
GAACTGATGT GGCCAGCAAA TCCTTCCTCC TGTTGTTACA ATGCTCCATA TCTCTCCGTG 
TATAGTGAAA ACGCAGTTGA TATCTTTGAT GTAAACTCCA TGGAATGGAT ACAGACTCTT 
CCTCTCAAAA AGGTCCGACC TTTAAACACT GAAGGATCAT TAAATCTCTT AGGATTGGAG 
ACCATTAGAT TAATATATTT CAAAAATAAG ATGGCAGAAG GGGATGAACT TGTGGTTCCT 
GAAACATCAG ATAATAGTCG GAAACAAATG GTTAGAAATA TCAACAACAA ACGGCGTTAC 
TCCTTCCGAG TCCCTGAAGA AGAGAGGATG CAACAGAGGA GGGAGATGCT ACGAGATCCA 
GAAATGAGAA ATAAATTAAT TTCTAACCCA ACTAATTTTA ACCACATAGC ACACATGGGT 
CCTGGAGATG GAATACAGAT CCTAAAAGAC CTGCCCATGA ACCCACGGCC TCAGGAAAGC 
CGGACAGTAT TCAGTGGCTC CGTCAGTATT CCCTCCATCA CCAAGTCCCG CCCTGAGCCA 
GGCCGCTCCA TGAGTGCCAG CAGCGGCCTG TCTGCACGGT CGTCAGCCCA GAATGGCAGT 
GCACTAAAGA GGGAATTCTC TGGAGGAAGC TATAACACAA AGCGACAGCC CATGCCCTCT 
CCCTCGGAGG GCTCTTTGTC ATCAGGAGGC ATGGACCAAG GAAGTGACGC CCCTGCGAGG 
GACTATGATG GAGAGGACTC TGATTCTCCA AGGCATTCCA CAGCTTCCAA CAGCTCTAAC 
CTGAGCAGCC CCCCAAGTCC CATTTCACCC CAAAAGACCA AGAGCCTCTC CCTGGAGAGC 
ACAGACCGCG GGAGCTGGGA TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346471.1
CDS	351..5597
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSLSSSSSSLIDFLDDRSPSCTPASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	NM_001359542.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359542.1

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAAGATTCC CTTTCTTCTT CCTCATCCTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC GTCCTGTACT CCAGCTAGCA AAGGCAGACG TATTGCAGAC 
TGTGCTCCAC TTCCAGCTCA CACACCAACC TTAAGGAAAA AGGGATGCCC GGCTTCAACT 
GGCTTTCCAC CTAAGCGCAA GACTCATCAA TTTTTCGTGA AGTCTTTCAC TGCCCCTACC 
AAGTGTCACC AGTGCACCTC CTTGATGGTC GGTTTGATAA GACAGGGCTG TTCCTGTGAA 
GTCTGTGGGT TCTCATGTCA TATAACTTGT GTGAACAAAG CCCCGACGGT TTGCCCAGTT 
CCTCCTGAGC AAACTAAAGG TCCACTGGGC ATAGACCCCC AGAAGGGAGT TGGGACGGCG 
TATGAGGGTC ATGTCAGGAT CCCTAAGCCG GCGGGAGTGA AGAAAGGGTG GCAGAGAGCA 
CTGGCTGTGG TCTGCGACTT CAAACTGTTT CTTTATGATA TCGCAGAGGG AAAAGCATCG 
CAGCCCACCT CCGTCATTAG TCAAGTGATT GACATGAGAG ATGAAGAATT TTCTGTGAGT 
TCAGTCTTAG CTTCTGATGT TATTCACGCA AGTCGAAAAG ATATCCCCTG TATATTCAGG 
GTCACAGCTT CCCAGCTCTC AGCACCTAGT AACAAATGTT CCATCCTGAT GCTAGCAGAC 
AGCGAGAATG AGAGGAGTAA GTGGGTGGGA GTGCTGAGTG AACTCCACAA GATTCTGAAG 
AAAAACAAGT TCAGAGACCG CTCAGTGTAT GTCCCCAAGG AGGCGTACGA CAGCACGCTA 
CCCCTCATTA AGACAACGCA GGCCGCTGCA ATCATAGATC ATGAACGGAT AGCTTTGGGA 
AATGAAGAAG GATTATTTGT TGTGCATGTC ACCAAAGATG AGATTGTTAG AGTTGGTGAT 
AATAAGAAAA TCCATCAGAT CGAACTCATT CCAAGTGACC AGCTTGTCGC TGTGATCTCA 
GGCCGAAATC GCCACGTCCG ACTTTTTCCT ATGTCAGCCT TGGATGGGCG AGAGACGGAT 
TTTTATAAAC TGGCAGAGAC TAAAGGGTGT CAGACCATAG CTGCTGGGAA AGTGCGCCAC 
GGAGCTCTGT CCTGCCTGTG TGTGGCCATG AAGAGACAGG TCCTCTGCTA TGAACTGTTC 
CAAAGCAAGA CCAGGCACAG GAAGTTTAAG GAGATTCAAG TCCCATGTAA TGTCCAGTGG 
ATGGCCATCT TCAGTGAGCA TCTCTGTGTG GGATTCCAGT CAGGATTTCT CAGATACCCC 
CTGAATGGAG AAGGAGGTCC ATGCAATATG CTCCATTCAA ATGACCATAC ACTGTCATTC 
ATTTCACACC AACCAATGGA CGCTCTCTGT GCAGTTGAGA TCTCCAACAA AGAGTACTTG 
CTGTGTTTTA ACAGCATTGG GATATACACA GACTGCCAGG GCCGAAGATC CAGACAGCAG 
GAACTGATGT GGCCAGCAAA TCCTTCCTCC TGTTGTTACA ATGCTCCATA TCTCTCCGTG 
TATAGTGAAA ACGCAGTTGA TATCTTTGAT GTAAACTCCA TGGAATGGAT ACAGACTCTT 
CCTCTCAAAA AGGTCCGACC TTTAAACACT GAAGGATCAT TAAATCTCTT AGGATTGGAG 
ACCATTAGAT TAATATATTT CAAAAATAAG ATGGCAGAAG GGGATGAACT TGTGGTTCCT 
GAAACATCAG ATAATAGTCG GAAACAAATG GTTAGAAATA TCAACAACAA ACGGCGTTAC 
TCCTTCCGAG TCCCTGAAGA AGAGAGGATG CAACAGAGGA GGGAGATGCT ACGAGATCCA 
GAAATGAGAA ATAAATTAAT TTCTAACCCA ACTAATTTTA ACCACATAGC ACACATGGGT 
CCTGGAGATG GAATACAGAT CCTAAAAGAC CTGCCCATGA ACCCACGGCC TCAGGAAAGC 
CGGACAGTAT TCAGTGGCTC CGTCAGTATT CCCTCCATCA CCAAGTCCCG CCCTGAGCCA 
GGCCGCTCCA TGAGTGCCAG CAGCGGCCTG TCTGCACGGT CGTCAGCCCA GAATGGCAGT 
GCACTAAAGA GGGAATTCTC TGGAGGAAGC TATAACACAA AGCGACAGCC CATGCCCTCT 
CCCTCGGAGG GCTCTTTGTC ATCAGGAGGC ATGGACCAAG GAAGTGACGC CCCTGCGAGG 
GACTATGATG GAGAGGACTC TGATTCTCCA AGGCATTCCA CAGCTTCCAA CAGCTCTAAC 
CTGAGCAGCC CCCCAAGTCC CATTTCACCC CAAAAGACCA AGAGCCTCTC CCTGGAGAGC 
ACAGACCGCG GGAGCTGGGA TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( NM_001346804.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001333733.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 2, mRNA.

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CloneID	OMu28730
Clone ID Related Accession (Same CDS sequence)	NM_001346804.1
Accession Version	NM_001346804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC125380.4 and AC132436.3. On Oct 30, 2016 this sequence version replaced XM_006496810.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.234626.1, BC158095.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAAATTGCA GACTGTGCTC CACTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG CCCGGCTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAT 
CAATTTTTCG TGAAGTCTTT CACTGCCCCT ACCAAGTGTC ACCAGTGCAC CTCCTTGATG 
GTCGGTTTGA TAAGACAGGG CTGTTCCTGT GAAGTCTGTG GGTTCTCATG TCATATAACT 
TGTGTGAACA AAGCCCCGAC GGTTTGCCCA GTTCCTCCTG AGCAAACTAA AGGTCCACTG 
GGCATAGACC CCCAGAAGGG AGTTGGGACG GCGTATGAGG GTCATGTCAG GATCCCTAAG 
CCGGCGGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTG TGGTCTGCGA CTTCAAACTG 
TTTCTTTATG ATATCGCAGA GGGAAAAGCA TCGCAGCCCA CCTCCGTCAT TAGTCAAGTG 
ATTGACATGA GAGATGAAGA ATTTTCTGTG AGTTCAGTCT TAGCTTCTGA TGTTATTCAC 
GCAAGTCGAA AAGATATCCC CTGTATATTC AGGGTCACAG CTTCCCAGCT CTCAGCACCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCA GACAGCGAGA ATGAGAGGAG TAAGTGGGTG 
GGAGTGCTGA GTGAACTCCA CAAGATTCTG AAGAAAAACA AGTTCAGAGA CCGCTCAGTG 
TATGTCCCCA AGGAGGCGTA CGACAGCACG CTACCCCTCA TTAAGACAAC GCAGGCCGCT 
GCAATCATAG ATCATGAACG GATAGCTTTG GGAAATGAAG AAGGATTATT TGTTGTGCAT 
GTCACCAAAG ATGAGATTGT TAGAGTTGGT GATAATAAGA AAATCCATCA GATCGAACTC 
ATTCCAAGTG ACCAGCTTGT CGCTGTGATC TCAGGCCGAA ATCGCCACGT CCGACTTTTT 
CCTATGTCAG CCTTGGATGG GCGAGAGACG GATTTTTATA AACTGGCAGA GACTAAAGGG 
TGTCAGACCA TAGCTGCTGG GAAAGTGCGC CACGGAGCTC TGTCCTGCCT GTGTGTGGCC 
ATGAAGAGAC AGGTCCTCTG CTATGAACTG TTCCAAAGCA AGACCAGGCA CAGGAAGTTT 
AAGGAGATTC AAGTCCCATG TAATGTCCAG TGGATGGCCA TCTTCAGTGA GCATCTCTGT 
GTGGGATTCC AGTCAGGATT TCTCAGATAC CCCCTGAATG GAGAAGGAGG TCCATGCAAT 
ATGCTCCATT CAAATGACCA TACACTGTCA TTCATTTCAC ACCAACCAAT GGACGCTCTC 
TGTGCAGTTG AGATCTCCAA CAAAGAGTAC TTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACAGACTGCC AGGGCCGAAG ATCCAGACAG CAGGAACTGA TGTGGCCAGC AAATCCTTCC 
TCCTGTTGTT ACAATGCTCC ATATCTCTCC GTGTATAGTG AAAACGCAGT TGATATCTTT 
GATGTAAACT CCATGGAATG GATACAGACT CTTCCTCTCA AAAAGGTCCG ACCTTTAAAC 
ACTGAAGGAT CATTAAATCT CTTAGGATTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTTGTGGTT CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ATATCAACAA CAAACGGCGT TACTCCTTCC GAGTCCCTGA AGAAGAGAGG 
ATGCAACAGA GGAGGGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGAACCCACG GCCTCAGGAA AGCCGGACAG TATTCAGTGG CTCCGTCAGT 
ATTCCCTCCA TCACCAAGTC CCGCCCTGAG CCAGGCCGCT CCATGAGTGC CAGCAGCGGC 
CTGTCTGCAC GGTCGTCAGC CCAGAATGGC AGTGCACTAA AGAGGGAATT CTCTGGAGGA 
AGCTATAACA CAAAGCGACA GCCCATGCCC TCTCCCTCGG AGGGCTCTTT GTCATCAGGA 
GGCATGGACC AAGGAAGTGA CGCCCCTGCG AGGGACTATG ATGGAGAGGA CTCTGATTCT 
CCAAGGCATT CCACAGCTTC CAACAGCTCT AACCTGAGCA GCCCCCCAAG TCCCATTTCA 
CCCCAAAAGA CCAAGAGCCT CTCCCTGGAG AGCACAGACC GCGGGAGCTG GGATCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333733.1
CDS	351..5510
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	NM_001346804.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346804.1

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAAATTGCA GACTGTGCTC CACTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG CCCGGCTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAT 
CAATTTTTCG TGAAGTCTTT CACTGCCCCT ACCAAGTGTC ACCAGTGCAC CTCCTTGATG 
GTCGGTTTGA TAAGACAGGG CTGTTCCTGT GAAGTCTGTG GGTTCTCATG TCATATAACT 
TGTGTGAACA AAGCCCCGAC GGTTTGCCCA GTTCCTCCTG AGCAAACTAA AGGTCCACTG 
GGCATAGACC CCCAGAAGGG AGTTGGGACG GCGTATGAGG GTCATGTCAG GATCCCTAAG 
CCGGCGGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTG TGGTCTGCGA CTTCAAACTG 
TTTCTTTATG ATATCGCAGA GGGAAAAGCA TCGCAGCCCA CCTCCGTCAT TAGTCAAGTG 
ATTGACATGA GAGATGAAGA ATTTTCTGTG AGTTCAGTCT TAGCTTCTGA TGTTATTCAC 
GCAAGTCGAA AAGATATCCC CTGTATATTC AGGGTCACAG CTTCCCAGCT CTCAGCACCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCA GACAGCGAGA ATGAGAGGAG TAAGTGGGTG 
GGAGTGCTGA GTGAACTCCA CAAGATTCTG AAGAAAAACA AGTTCAGAGA CCGCTCAGTG 
TATGTCCCCA AGGAGGCGTA CGACAGCACG CTACCCCTCA TTAAGACAAC GCAGGCCGCT 
GCAATCATAG ATCATGAACG GATAGCTTTG GGAAATGAAG AAGGATTATT TGTTGTGCAT 
GTCACCAAAG ATGAGATTGT TAGAGTTGGT GATAATAAGA AAATCCATCA GATCGAACTC 
ATTCCAAGTG ACCAGCTTGT CGCTGTGATC TCAGGCCGAA ATCGCCACGT CCGACTTTTT 
CCTATGTCAG CCTTGGATGG GCGAGAGACG GATTTTTATA AACTGGCAGA GACTAAAGGG 
TGTCAGACCA TAGCTGCTGG GAAAGTGCGC CACGGAGCTC TGTCCTGCCT GTGTGTGGCC 
ATGAAGAGAC AGGTCCTCTG CTATGAACTG TTCCAAAGCA AGACCAGGCA CAGGAAGTTT 
AAGGAGATTC AAGTCCCATG TAATGTCCAG TGGATGGCCA TCTTCAGTGA GCATCTCTGT 
GTGGGATTCC AGTCAGGATT TCTCAGATAC CCCCTGAATG GAGAAGGAGG TCCATGCAAT 
ATGCTCCATT CAAATGACCA TACACTGTCA TTCATTTCAC ACCAACCAAT GGACGCTCTC 
TGTGCAGTTG AGATCTCCAA CAAAGAGTAC TTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACAGACTGCC AGGGCCGAAG ATCCAGACAG CAGGAACTGA TGTGGCCAGC AAATCCTTCC 
TCCTGTTGTT ACAATGCTCC ATATCTCTCC GTGTATAGTG AAAACGCAGT TGATATCTTT 
GATGTAAACT CCATGGAATG GATACAGACT CTTCCTCTCA AAAAGGTCCG ACCTTTAAAC 
ACTGAAGGAT CATTAAATCT CTTAGGATTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTTGTGGTT CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ATATCAACAA CAAACGGCGT TACTCCTTCC GAGTCCCTGA AGAAGAGAGG 
ATGCAACAGA GGAGGGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGAACCCACG GCCTCAGGAA AGCCGGACAG TATTCAGTGG CTCCGTCAGT 
ATTCCCTCCA TCACCAAGTC CCGCCCTGAG CCAGGCCGCT CCATGAGTGC CAGCAGCGGC 
CTGTCTGCAC GGTCGTCAGC CCAGAATGGC AGTGCACTAA AGAGGGAATT CTCTGGAGGA 
AGCTATAACA CAAAGCGACA GCCCATGCCC TCTCCCTCGG AGGGCTCTTT GTCATCAGGA 
GGCATGGACC AAGGAAGTGA CGCCCCTGCG AGGGACTATG ATGGAGAGGA CTCTGATTCT 
CCAAGGCATT CCACAGCTTC CAACAGCTCT AACCTGAGCA GCCCCCCAAG TCCCATTTCA 
CCCCAAAAGA CCAAGAGCCT CTCCCTGGAG AGCACAGACC GCGGGAGCTG GGATCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( NM_001359545.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346474.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 8, mRNA.

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CloneID	OMu28732
Clone ID Related Accession (Same CDS sequence)	NM_001359545.1 , XM_006496812.3
Accession Version	NM_001359545.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform 8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC125380.4 and AC132436.3. On Jan 13, 2018 this sequence version replaced XM_006496812.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.105487.1, SRR1660811.211768.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGCTA GCAAAGGCAG ACGTATTGCA GACTGTGCTC CACTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG CCCGGCTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAT 
CAATTTTTCG TGAAGTCTTT CACTGCCCCT ACCAAGTGTC ACCAGTGCAC CTCCTTGATG 
GTCGGTTTGA TAAGACAGGG CTGTTCCTGT GAAGTCTGTG GGTTCTCATG TCATATAACT 
TGTGTGAACA AAGCCCCGAC GGTTTGCCCA GTTCCTCCTG AGCAAACTAA AGGTCCACTG 
GGCATAGACC CCCAGAAGGG AGTTGGGACG GCGTATGAGG GTCATGTCAG GATCCCTAAG 
CCGGCGGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTG TGGTCTGCGA CTTCAAACTG 
TTTCTTTATG ATATCGCAGA GGGAAAAGCA TCGCAGCCCA CCTCCGTCAT TAGTCAAGTG 
ATTGACATGA GAGATGAAGA ATTTTCTGTG AGTTCAGTCT TAGCTTCTGA TGTTATTCAC 
GCAAGTCGAA AAGATATCCC CTGTATATTC AGGGTCACAG CTTCCCAGCT CTCAGCACCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCA GACAGCGAGA ATGAGAGGAG TAAGTGGGTG 
GGAGTGCTGA GTGAACTCCA CAAGATTCTG AAGAAAAACA AGTTCAGAGA CCGCTCAGTG 
TATGTCCCCA AGGAGGCGTA CGACAGCACG CTACCCCTCA TTAAGACAAC GCAGGCCGCT 
GCAATCATAG ATCATGAACG GATAGCTTTG GGAAATGAAG AAGGATTATT TGTTGTGCAT 
GTCACCAAAG ATGAGATTGT TAGAGTTGGT GATAATAAGA AAATCCATCA GATCGAACTC 
ATTCCAAGTG ACCAGCTTGT CGCTGTGATC TCAGGCCGAA ATCGCCACGT CCGACTTTTT 
CCTATGTCAG CCTTGGATGG GCGAGAGACG GATTTTTATA AACTGGCAGA GACTAAAGGG 
TGTCAGACCA TAGCTGCTGG GAAAGTGCGC CACGGAGCTC TGTCCTGCCT GTGTGTGGCC 
ATGAAGAGAC AGGTCCTCTG CTATGAACTG TTCCAAAGCA AGACCAGGCA CAGGAAGTTT 
AAGGAGATTC AAGTCCCATG TAATGTCCAG TGGATGGCCA TCTTCAGTGA GCATCTCTGT 
GTGGGATTCC AGTCAGGATT TCTCAGATAC CCCCTGAATG GAGAAGGAGG TCCATGCAAT 
ATGCTCCATT CAAATGACCA TACACTGTCA TTCATTTCAC ACCAACCAAT GGACGCTCTC 
TGTGCAGTTG AGATCTCCAA CAAAGAGTAC TTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACAGACTGCC AGGGCCGAAG ATCCAGACAG CAGGAACTGA TGTGGCCAGC AAATCCTTCC 
TCCTGTTGTT ACAATGCTCC ATATCTCTCC GTGTATAGTG AAAACGCAGT TGATATCTTT 
GATGTAAACT CCATGGAATG GATACAGACT CTTCCTCTCA AAAAGGTCCG ACCTTTAAAC 
ACTGAAGGAT CATTAAATCT CTTAGGATTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTTGTGGTT CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ATATCAACAA CAAACGGCGT TACTCCTTCC GAGTCCCTGA AGAAGAGAGG 
ATGCAACAGA GGAGGGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGAACCCACG GCCTCAGGAA AGCCGGACAG TATTCAGTGG CTCCGTCAGT 
ATTCCCTCCA TCACCAAGTC CCGCCCTGAG CCAGGCCGCT CCATGAGTGC CAGCAGCGGC 
CTGTCTGCAC GGTCGTCAGC CCAGAATGGC AGTGCACTAA AGAGGGAATT CTCTGGAGGA 
AGCTATAACA CAAAGCGACA GCCCATGCCC TCTCCCTCGG AGGGCTCTTT GTCATCAGGA 
GGCATGGACC AAGGAAGTGA CGCCCCTGCG AGGGACTATG ATGGAGAGGA CTCTGATTCT 
CCAAGGCATT CCACAGCTTC CAACAGCTCT AACCTGAGCA GCCCCCCAAG TCCCATTTCA 
CCCCAAAAGA CCAAGAGCCT CTCCCTGGAG AGCACAGACC GCGGGAGCTG GGATCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346474.1
CDS	351..5450
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	NM_001359545.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359545.1

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGCTA GCAAAGGCAG ACGTATTGCA GACTGTGCTC CACTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG CCCGGCTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAT 
CAATTTTTCG TGAAGTCTTT CACTGCCCCT ACCAAGTGTC ACCAGTGCAC CTCCTTGATG 
GTCGGTTTGA TAAGACAGGG CTGTTCCTGT GAAGTCTGTG GGTTCTCATG TCATATAACT 
TGTGTGAACA AAGCCCCGAC GGTTTGCCCA GTTCCTCCTG AGCAAACTAA AGGTCCACTG 
GGCATAGACC CCCAGAAGGG AGTTGGGACG GCGTATGAGG GTCATGTCAG GATCCCTAAG 
CCGGCGGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTG TGGTCTGCGA CTTCAAACTG 
TTTCTTTATG ATATCGCAGA GGGAAAAGCA TCGCAGCCCA CCTCCGTCAT TAGTCAAGTG 
ATTGACATGA GAGATGAAGA ATTTTCTGTG AGTTCAGTCT TAGCTTCTGA TGTTATTCAC 
GCAAGTCGAA AAGATATCCC CTGTATATTC AGGGTCACAG CTTCCCAGCT CTCAGCACCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCA GACAGCGAGA ATGAGAGGAG TAAGTGGGTG 
GGAGTGCTGA GTGAACTCCA CAAGATTCTG AAGAAAAACA AGTTCAGAGA CCGCTCAGTG 
TATGTCCCCA AGGAGGCGTA CGACAGCACG CTACCCCTCA TTAAGACAAC GCAGGCCGCT 
GCAATCATAG ATCATGAACG GATAGCTTTG GGAAATGAAG AAGGATTATT TGTTGTGCAT 
GTCACCAAAG ATGAGATTGT TAGAGTTGGT GATAATAAGA AAATCCATCA GATCGAACTC 
ATTCCAAGTG ACCAGCTTGT CGCTGTGATC TCAGGCCGAA ATCGCCACGT CCGACTTTTT 
CCTATGTCAG CCTTGGATGG GCGAGAGACG GATTTTTATA AACTGGCAGA GACTAAAGGG 
TGTCAGACCA TAGCTGCTGG GAAAGTGCGC CACGGAGCTC TGTCCTGCCT GTGTGTGGCC 
ATGAAGAGAC AGGTCCTCTG CTATGAACTG TTCCAAAGCA AGACCAGGCA CAGGAAGTTT 
AAGGAGATTC AAGTCCCATG TAATGTCCAG TGGATGGCCA TCTTCAGTGA GCATCTCTGT 
GTGGGATTCC AGTCAGGATT TCTCAGATAC CCCCTGAATG GAGAAGGAGG TCCATGCAAT 
ATGCTCCATT CAAATGACCA TACACTGTCA TTCATTTCAC ACCAACCAAT GGACGCTCTC 
TGTGCAGTTG AGATCTCCAA CAAAGAGTAC TTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACAGACTGCC AGGGCCGAAG ATCCAGACAG CAGGAACTGA TGTGGCCAGC AAATCCTTCC 
TCCTGTTGTT ACAATGCTCC ATATCTCTCC GTGTATAGTG AAAACGCAGT TGATATCTTT 
GATGTAAACT CCATGGAATG GATACAGACT CTTCCTCTCA AAAAGGTCCG ACCTTTAAAC 
ACTGAAGGAT CATTAAATCT CTTAGGATTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTTGTGGTT CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ATATCAACAA CAAACGGCGT TACTCCTTCC GAGTCCCTGA AGAAGAGAGG 
ATGCAACAGA GGAGGGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGAACCCACG GCCTCAGGAA AGCCGGACAG TATTCAGTGG CTCCGTCAGT 
ATTCCCTCCA TCACCAAGTC CCGCCCTGAG CCAGGCCGCT CCATGAGTGC CAGCAGCGGC 
CTGTCTGCAC GGTCGTCAGC CCAGAATGGC AGTGCACTAA AGAGGGAATT CTCTGGAGGA 
AGCTATAACA CAAAGCGACA GCCCATGCCC TCTCCCTCGG AGGGCTCTTT GTCATCAGGA 
GGCATGGACC AAGGAAGTGA CGCCCCTGCG AGGGACTATG ATGGAGAGGA CTCTGATTCT 
CCAAGGCATT CCACAGCTTC CAACAGCTCT AACCTGAGCA GCCCCCCAAG TCCCATTTCA 
CCCCAAAAGA CCAAGAGCCT CTCCCTGGAG AGCACAGACC GCGGGAGCTG GGATCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( NM_001346805.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001333734.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 3, mRNA.

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CloneID	OMu28733
Clone ID Related Accession (Same CDS sequence)	NM_001346805.1
Accession Version	NM_001346805.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC125380.4 and AC132436.3. On Oct 30, 2016 this sequence version replaced XM_006496813.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.191362.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGCTG GAAGTTCACA CCGAAGCTCT AATTGCTGAA 
GCATCAAAGG ACAAGAAGCT CCGAGAGCAG AGCGAACATT ATTCTAAGCA ACTGGAAAAC 
GAGTTGGAGG GCCTGAAGCA AAAACAAATT AGTTACTCAC CAGGAATATG CAGCATAGAA 
CATCAGCAAG AGATAACTAA ACTGAAAACT GACTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAAATTT CTAAAAGAGA AGGAATTCAC GCAAGTGAGA TTAAAAACCT GAAGAAGGAG 
CTGCATGACT CAGAAGGCCA GCAGCTTGCT CTCAACAAAG AGATTCTGGT TTTAAAAGAC 
AAATTGGAAA AAACCAGGAG AGAAAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAACAACAAT ATGAACGGGA AAAAGTATTA TTAACTGAAG AGAATAAAAA GTTAACGAGT 
GAACTCGATA AGCTGACTTC ACTGTATGAG AGCTTAAGTT TGCGCAACCA GCACTTAGAA 
GAAGAAGTCA AAGATCTAGC AGATAAGAAG GAATCAGTTG CCCACTGGGA AGCCCAAATC 
ACAGAGATAA TTCAGTGGGT GAGCGATGAG AAGGATGCAC GAGGCTATCT TCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA GTTGGAAGCA TTAAGAAATT CCAGCTTGGG GACACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCAAAACTGG ACATGTCGGC TAGACTAGAG 
TTGCAGTCTG CTCTGGATGC AGAAATAAGA GCTAAGCAGG CCATCCAGGA AGAGCTGAAT 
AAAGTCAAAG CATCTAACAT CTTAACAGAA TGTAAACTGA AAGATTCAGA GAAGAAGAAC 
TTGGAACTGT TGTCCGAAAT CGAGCAGCTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ACCAATTTGA AATTGCAGAC TGTGCTCCAC TTCCAGCTCA CACACCAACC 
TTAAGGAAAA AGGGATGCCC GGCTTCAACT GGCTTTCCAC CTAAGCGCAA GACTCATCAA 
TTTTTCGTGA AGTCTTTCAC TGCCCCTACC AAGTGTCACC AGTGCACCTC CTTGATGGTC 
GGTTTGATAA GACAGGGCTG TTCCTGTGAA GTCTGTGGGT TCTCATGTCA TATAACTTGT 
GTGAACAAAG CCCCGACGGT TTGCCCAGTT CCTCCTGAGC AAACTAAAGG TCCACTGGGC 
ATAGACCCCC AGAAGGGAGT TGGGACGGCG TATGAGGGTC ATGTCAGGAT CCCTAAGCCG 
GCGGGAGTGA AGAAAGGGTG GCAGAGAGCA CTGGCTGTGG TCTGCGACTT CAAACTGTTT 
CTTTATGATA TCGCAGAGGG AAAAGCATCG CAGCCCACCT CCGTCATTAG TCAAGTGATT 
GACATGAGAG ATGAAGAATT TTCTGTGAGT TCAGTCTTAG CTTCTGATGT TATTCACGCA 
AGTCGAAAAG ATATCCCCTG TATATTCAGG GTCACAGCTT CCCAGCTCTC AGCACCTAGT 
AACAAATGTT CCATCCTGAT GCTAGCAGAC AGCGAGAATG AGAGGAGTAA GTGGGTGGGA 
GTGCTGAGTG AACTCCACAA GATTCTGAAG AAAAACAAGT TCAGAGACCG CTCAGTGTAT 
GTCCCCAAGG AGGCGTACGA CAGCACGCTA CCCCTCATTA AGACAACGCA GGCCGCTGCA 
ATCATAGATC ATGAACGGAT AGCTTTGGGA AATGAAGAAG GATTATTTGT TGTGCATGTC 
ACCAAAGATG AGATTGTTAG AGTTGGTGAT AATAAGAAAA TCCATCAGAT CGAACTCATT 
CCAAGTGACC AGCTTGTCGC TGTGATCTCA GGCCGAAATC GCCACGTCCG ACTTTTTCCT 
ATGTCAGCCT TGGATGGGCG AGAGACGGAT TTTTATAAAC TGGCAGAGAC TAAAGGGTGT 
CAGACCATAG CTGCTGGGAA AGTGCGCCAC GGAGCTCTGT CCTGCCTGTG TGTGGCCATG 
AAGAGACAGG TCCTCTGCTA TGAACTGTTC CAAAGCAAGA CCAGGCACAG GAAGTTTAAG 
GAGATTCAAG TCCCATGTAA TGTCCAGTGG ATGGCCATCT TCAGTGAGCA TCTCTGTGTG 
GGATTCCAGT CAGGATTTCT CAGATACCCC CTGAATGGAG AAGGAGGTCC ATGCAATATG 
CTCCATTCAA ATGACCATAC ACTGTCATTC ATTTCACACC AACCAATGGA CGCTCTCTGT 
GCAGTTGAGA TCTCCAACAA AGAGTACTTG CTGTGTTTTA ACAGCATTGG GATATACACA 
GACTGCCAGG GCCGAAGATC CAGACAGCAG GAACTGATGT GGCCAGCAAA TCCTTCCTCC 
TGTTGTTACA ATGCTCCATA TCTCTCCGTG TATAGTGAAA ACGCAGTTGA TATCTTTGAT 
GTAAACTCCA TGGAATGGAT ACAGACTCTT CCTCTCAAAA AGGTCCGACC TTTAAACACT 
GAAGGATCAT TAAATCTCTT AGGATTGGAG ACCATTAGAT TAATATATTT CAAAAATAAG 
ATGGCAGAAG GGGATGAACT TGTGGTTCCT GAAACATCAG ATAATAGTCG GAAACAAATG 
GTTAGAAATA TCAACAACAA ACGGCGTTAC TCCTTCCGAG TCCCTGAAGA AGAGAGGATG 
CAACAGAGGA GGGAGATGCT ACGAGATCCA GAAATGAGAA ATAAATTAAT TTCTAACCCA 
ACTAATTTTA ACCACATAGC ACACATGGGT CCTGGAGATG GAATACAGAT CCTAAAAGAC 
CTGCCCATGA ACCCACGGCC TCAGGAAAGC CGGACAGTAT TCAGTGGCTC CGTCAGTATT 
CCCTCCATCA CCAAGTCCCG CCCTGAGCCA GGCCGCTCCA TGAGTGCCAG CAGCGGCCTG 
TCTGCACGGT CGTCAGCCCA GAATGGCAGT GCACTAAAGA GGGAATTCTC TGGAGGAAGC 
TATAACACAA AGCGACAGCC CATGCCCTCT CCCTCGGAGG GCTCTTTGTC ATCAGGAGGC 
ATGGACCAAG GAAGTGACGC CCCTGCGAGG GACTATGATG GAGAGGACTC TGATTCTCCA 
AGGCATTCCA CAGCTTCCAA CAGCTCTAAC CTGAGCAGCC CCCCAAGTCC CATTTCACCC 
CAAAAGACCA AGAGCCTCTC CCTGGAGAGC ACAGACCGCG GGAGCTGGGA TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333734.1
CDS	351..5267
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKLEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	NM_001346805.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346805.1

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGCTG GAAGTTCACA CCGAAGCTCT AATTGCTGAA 
GCATCAAAGG ACAAGAAGCT CCGAGAGCAG AGCGAACATT ATTCTAAGCA ACTGGAAAAC 
GAGTTGGAGG GCCTGAAGCA AAAACAAATT AGTTACTCAC CAGGAATATG CAGCATAGAA 
CATCAGCAAG AGATAACTAA ACTGAAAACT GACTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAAATTT CTAAAAGAGA AGGAATTCAC GCAAGTGAGA TTAAAAACCT GAAGAAGGAG 
CTGCATGACT CAGAAGGCCA GCAGCTTGCT CTCAACAAAG AGATTCTGGT TTTAAAAGAC 
AAATTGGAAA AAACCAGGAG AGAAAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAACAACAAT ATGAACGGGA AAAAGTATTA TTAACTGAAG AGAATAAAAA GTTAACGAGT 
GAACTCGATA AGCTGACTTC ACTGTATGAG AGCTTAAGTT TGCGCAACCA GCACTTAGAA 
GAAGAAGTCA AAGATCTAGC AGATAAGAAG GAATCAGTTG CCCACTGGGA AGCCCAAATC 
ACAGAGATAA TTCAGTGGGT GAGCGATGAG AAGGATGCAC GAGGCTATCT TCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA GTTGGAAGCA TTAAGAAATT CCAGCTTGGG GACACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCAAAACTGG ACATGTCGGC TAGACTAGAG 
TTGCAGTCTG CTCTGGATGC AGAAATAAGA GCTAAGCAGG CCATCCAGGA AGAGCTGAAT 
AAAGTCAAAG CATCTAACAT CTTAACAGAA TGTAAACTGA AAGATTCAGA GAAGAAGAAC 
TTGGAACTGT TGTCCGAAAT CGAGCAGCTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ACCAATTTGA AATTGCAGAC TGTGCTCCAC TTCCAGCTCA CACACCAACC 
TTAAGGAAAA AGGGATGCCC GGCTTCAACT GGCTTTCCAC CTAAGCGCAA GACTCATCAA 
TTTTTCGTGA AGTCTTTCAC TGCCCCTACC AAGTGTCACC AGTGCACCTC CTTGATGGTC 
GGTTTGATAA GACAGGGCTG TTCCTGTGAA GTCTGTGGGT TCTCATGTCA TATAACTTGT 
GTGAACAAAG CCCCGACGGT TTGCCCAGTT CCTCCTGAGC AAACTAAAGG TCCACTGGGC 
ATAGACCCCC AGAAGGGAGT TGGGACGGCG TATGAGGGTC ATGTCAGGAT CCCTAAGCCG 
GCGGGAGTGA AGAAAGGGTG GCAGAGAGCA CTGGCTGTGG TCTGCGACTT CAAACTGTTT 
CTTTATGATA TCGCAGAGGG AAAAGCATCG CAGCCCACCT CCGTCATTAG TCAAGTGATT 
GACATGAGAG ATGAAGAATT TTCTGTGAGT TCAGTCTTAG CTTCTGATGT TATTCACGCA 
AGTCGAAAAG ATATCCCCTG TATATTCAGG GTCACAGCTT CCCAGCTCTC AGCACCTAGT 
AACAAATGTT CCATCCTGAT GCTAGCAGAC AGCGAGAATG AGAGGAGTAA GTGGGTGGGA 
GTGCTGAGTG AACTCCACAA GATTCTGAAG AAAAACAAGT TCAGAGACCG CTCAGTGTAT 
GTCCCCAAGG AGGCGTACGA CAGCACGCTA CCCCTCATTA AGACAACGCA GGCCGCTGCA 
ATCATAGATC ATGAACGGAT AGCTTTGGGA AATGAAGAAG GATTATTTGT TGTGCATGTC 
ACCAAAGATG AGATTGTTAG AGTTGGTGAT AATAAGAAAA TCCATCAGAT CGAACTCATT 
CCAAGTGACC AGCTTGTCGC TGTGATCTCA GGCCGAAATC GCCACGTCCG ACTTTTTCCT 
ATGTCAGCCT TGGATGGGCG AGAGACGGAT TTTTATAAAC TGGCAGAGAC TAAAGGGTGT 
CAGACCATAG CTGCTGGGAA AGTGCGCCAC GGAGCTCTGT CCTGCCTGTG TGTGGCCATG 
AAGAGACAGG TCCTCTGCTA TGAACTGTTC CAAAGCAAGA CCAGGCACAG GAAGTTTAAG 
GAGATTCAAG TCCCATGTAA TGTCCAGTGG ATGGCCATCT TCAGTGAGCA TCTCTGTGTG 
GGATTCCAGT CAGGATTTCT CAGATACCCC CTGAATGGAG AAGGAGGTCC ATGCAATATG 
CTCCATTCAA ATGACCATAC ACTGTCATTC ATTTCACACC AACCAATGGA CGCTCTCTGT 
GCAGTTGAGA TCTCCAACAA AGAGTACTTG CTGTGTTTTA ACAGCATTGG GATATACACA 
GACTGCCAGG GCCGAAGATC CAGACAGCAG GAACTGATGT GGCCAGCAAA TCCTTCCTCC 
TGTTGTTACA ATGCTCCATA TCTCTCCGTG TATAGTGAAA ACGCAGTTGA TATCTTTGAT 
GTAAACTCCA TGGAATGGAT ACAGACTCTT CCTCTCAAAA AGGTCCGACC TTTAAACACT 
GAAGGATCAT TAAATCTCTT AGGATTGGAG ACCATTAGAT TAATATATTT CAAAAATAAG 
ATGGCAGAAG GGGATGAACT TGTGGTTCCT GAAACATCAG ATAATAGTCG GAAACAAATG 
GTTAGAAATA TCAACAACAA ACGGCGTTAC TCCTTCCGAG TCCCTGAAGA AGAGAGGATG 
CAACAGAGGA GGGAGATGCT ACGAGATCCA GAAATGAGAA ATAAATTAAT TTCTAACCCA 
ACTAATTTTA ACCACATAGC ACACATGGGT CCTGGAGATG GAATACAGAT CCTAAAAGAC 
CTGCCCATGA ACCCACGGCC TCAGGAAAGC CGGACAGTAT TCAGTGGCTC CGTCAGTATT 
CCCTCCATCA CCAAGTCCCG CCCTGAGCCA GGCCGCTCCA TGAGTGCCAG CAGCGGCCTG 
TCTGCACGGT CGTCAGCCCA GAATGGCAGT GCACTAAAGA GGGAATTCTC TGGAGGAAGC 
TATAACACAA AGCGACAGCC CATGCCCTCT CCCTCGGAGG GCTCTTTGTC ATCAGGAGGC 
ATGGACCAAG GAAGTGACGC CCCTGCGAGG GACTATGATG GAGAGGACTC TGATTCTCCA 
AGGCATTCCA CAGCTTCCAA CAGCTCTAAC CTGAGCAGCC CCCCAAGTCC CATTTCACCC 
CAAAAGACCA AGAGCCTCTC CCTGGAGAGC ACAGACCGCG GGAGCTGGGA TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_017320119.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175608.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X1, mRNA.

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CloneID	OMu28721
Clone ID Related Accession (Same CDS sequence)	XM_017320119.2 , XM_017320119.1 , XM_006496801.3
Accession Version	XM_017320119.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAAGATTCC CTTTCTTCTT CCTCATCCTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC GTCCTGTACT CCAGCTAGCA AAGGCAGACG TATTGCAGAC 
TGTGCTCCAC TTCCAGCTCA CACACCAACC TTAAGGAAAA AGGGATGCCC GGCTTCAACT 
GGCTTTCCAC CTAAGCGCAA GACTCATCAA TTTTTCGTGA AGTCTTTCAC TGCCCCTACC 
AAGTGTCACC AGTGCACCTC CTTGATGGTC GGTTTGATAA GACAGGGCTG TTCCTGTGAA 
GTCTGTGGGT TCTCATGTCA TATAACTTGT GTGAACAAAG CCCCGACGGT TTGCCCAGTT 
CCTCCTGAGC AAACTAAAGG TCCACTGGGC ATAGACCCCC AGAAGGGAGT TGGGACGGCG 
TATGAGGGTC ATGTCAGGAT CCCTAAGCCG GCGGGAGTGA AGAAAGGGTG GCAGAGAGCA 
CTGGCTGTGG TCTGCGACTT CAAACTGTTT CTTTATGATA TCGCAGAGGG AAAAGCATCG 
CAGCCCACCT CCGTCATTAG TCAAGTGATT GACATGAGAG ATGAAGAATT TTCTGTGAGT 
TCAGTCTTAG CTTCTGATGT TATTCACGCA AGTCGAAAAG ATATCCCCTG TATATTCAGG 
GTCACAGCTT CCCAGCTCTC AGCACCTAGT AACAAATGTT CCATCCTGAT GCTAGCAGAC 
AGCGAGAATG AGAGGAGTAA GTGGGTGGGA GTGCTGAGTG AACTCCACAA GATTCTGAAG 
AAAAACAAGT TCAGAGACCG CTCAGTGTAT GTCCCCAAGG AGGCGTACGA CAGCACGCTA 
CCCCTCATTA AGACAACGCA GGCCGCTGCA ATCATAGATC ATGAACGGAT AGCTTTGGGA 
AATGAAGAAG GATTATTTGT TGTGCATGTC ACCAAAGATG AGATTGTTAG AGTTGGTGAT 
AATAAGAAAA TCCATCAGAT CGAACTCATT CCAAGTGACC AGCTTGTCGC TGTGATCTCA 
GGCCGAAATC GCCACGTCCG ACTTTTTCCT ATGTCAGCCT TGGATGGGCG AGAGACGGAT 
TTTTATAAAC TGGCAGAGAC TAAAGGGTGT CAGACCATAG CTGCTGGGAA AGTGCGCCAC 
GGAGCTCTGT CCTGCCTGTG TGTGGCCATG AAGAGACAGG TCCTCTGCTA TGAACTGTTC 
CAAAGCAAGA CCAGGCACAG GAAGTTTAAG GAGATTCAAG TCCCATGTAA TGTCCAGTGG 
ATGGCCATCT TCAGTGAGCA TCTCTGTGTG GGATTCCAGT CAGGATTTCT CAGATACCCC 
CTGAATGGAG AAGGAGGTCC ATGCAATATG CTCCATTCAA ATGACCATAC ACTGTCATTC 
ATTTCACACC AACCAATGGA CGCTCTCTGT GCAGTTGAGA TCTCCAACAA AGAGTACTTG 
CTGTGTTTTA ACAGCATTGG GATATACACA GACTGCCAGG GCCGAAGATC CAGACAGCAG 
GAACTGATGT GGCCAGCAAA TCCTTCCTCC TGTTGTTACA ATGCTCCATA TCTCTCCGTG 
TATAGTGAAA ACGCAGTTGA TATCTTTGAT GTAAACTCCA TGGAATGGAT ACAGACTCTT 
CCTCTCAAAA AGGTCCGACC TTTAAACACT GAAGGATCAT TAAATCTCTT AGGATTGGAG 
ACCATTAGAT TAATATATTT CAAAAATAAG ATGGCAGAAG GGGATGAACT TGTGGTTCCT 
GAAACATCAG ATAATAGTCG GAAACAAATG GTTAGAAATA TCAACAACAA ACGGCGTTAC 
TCCTTCCGAG TCCCTGAAGA AGAGAGGATG CAACAGAGGA GGGAGATGCT ACGAGATCCA 
GAAATGAGAA ATAAATTAAT TTCTAACCCA ACTAATTTTA ACCACATAGC ACACATGGGT 
CCTGGAGATG GAATACAGAT CCTAAAAGAC CTGCCCATGC CAGGTTTCCC TTATTCACCC 
CCTCATCACC ACTCCGGCCT GATCTCCTCT CCGAGCAACT TTGAGCACAT CTATCACATG 
ACTGTCACTT CTGCTGAACA GTTTCTCTCT CCTGAGTCCA TAAGCCCTGG GTCCCCCCCG 
TCCCTGCGCT CTGCCCCTGG CACACCACAC TCTGTGACTC TGAGGAACCC ACGGCCTCAG 
GAAAGCCGGA CAGTATTCAG TGGCTCCGTC AGTATTCCCT CCATCACCAA GTCCCGCCCT 
GAGCCAGGCC GCTCCATGAG TGCCAGCAGC GGCCTGTCTG CACGGTCGTC AGCCCAGAAT 
GGCAGTGCAC TAAAGAGGGA ATTCTCTGGA GGAAGCTATA ACACAAAGCG ACAGCCCATG 
CCCTCTCCCT CGGAGGGCTC TTTGTCATCA GGAGGCATGG ACCAAGGAAG TGACGCCCCT 
GCGAGGGACT ATGATGGAGA GGACTCTGAT TCTCCAAGGC ATTCCACAGC TTCCAACAGC 
TCTAACCTGA GCAGCCCCCC AAGTCCCATT TCACCCCAAA AGACCAAGAG CCTCTCCCTG 
GAGAGCACAG ACCGCGGGAG CTGGGATCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175608.1
CDS	1142..6574
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSLSSSSSSLIDFLDDRSPSCTPASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_017320119.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320119.1

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAAGATTCC CTTTCTTCTT CCTCATCCTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC GTCCTGTACT CCAGCTAGCA AAGGCAGACG TATTGCAGAC 
TGTGCTCCAC TTCCAGCTCA CACACCAACC TTAAGGAAAA AGGGATGCCC GGCTTCAACT 
GGCTTTCCAC CTAAGCGCAA GACTCATCAA TTTTTCGTGA AGTCTTTCAC TGCCCCTACC 
AAGTGTCACC AGTGCACCTC CTTGATGGTC GGTTTGATAA GACAGGGCTG TTCCTGTGAA 
GTCTGTGGGT TCTCATGTCA TATAACTTGT GTGAACAAAG CCCCGACGGT TTGCCCAGTT 
CCTCCTGAGC AAACTAAAGG TCCACTGGGC ATAGACCCCC AGAAGGGAGT TGGGACGGCG 
TATGAGGGTC ATGTCAGGAT CCCTAAGCCG GCGGGAGTGA AGAAAGGGTG GCAGAGAGCA 
CTGGCTGTGG TCTGCGACTT CAAACTGTTT CTTTATGATA TCGCAGAGGG AAAAGCATCG 
CAGCCCACCT CCGTCATTAG TCAAGTGATT GACATGAGAG ATGAAGAATT TTCTGTGAGT 
TCAGTCTTAG CTTCTGATGT TATTCACGCA AGTCGAAAAG ATATCCCCTG TATATTCAGG 
GTCACAGCTT CCCAGCTCTC AGCACCTAGT AACAAATGTT CCATCCTGAT GCTAGCAGAC 
AGCGAGAATG AGAGGAGTAA GTGGGTGGGA GTGCTGAGTG AACTCCACAA GATTCTGAAG 
AAAAACAAGT TCAGAGACCG CTCAGTGTAT GTCCCCAAGG AGGCGTACGA CAGCACGCTA 
CCCCTCATTA AGACAACGCA GGCCGCTGCA ATCATAGATC ATGAACGGAT AGCTTTGGGA 
AATGAAGAAG GATTATTTGT TGTGCATGTC ACCAAAGATG AGATTGTTAG AGTTGGTGAT 
AATAAGAAAA TCCATCAGAT CGAACTCATT CCAAGTGACC AGCTTGTCGC TGTGATCTCA 
GGCCGAAATC GCCACGTCCG ACTTTTTCCT ATGTCAGCCT TGGATGGGCG AGAGACGGAT 
TTTTATAAAC TGGCAGAGAC TAAAGGGTGT CAGACCATAG CTGCTGGGAA AGTGCGCCAC 
GGAGCTCTGT CCTGCCTGTG TGTGGCCATG AAGAGACAGG TCCTCTGCTA TGAACTGTTC 
CAAAGCAAGA CCAGGCACAG GAAGTTTAAG GAGATTCAAG TCCCATGTAA TGTCCAGTGG 
ATGGCCATCT TCAGTGAGCA TCTCTGTGTG GGATTCCAGT CAGGATTTCT CAGATACCCC 
CTGAATGGAG AAGGAGGTCC ATGCAATATG CTCCATTCAA ATGACCATAC ACTGTCATTC 
ATTTCACACC AACCAATGGA CGCTCTCTGT GCAGTTGAGA TCTCCAACAA AGAGTACTTG 
CTGTGTTTTA ACAGCATTGG GATATACACA GACTGCCAGG GCCGAAGATC CAGACAGCAG 
GAACTGATGT GGCCAGCAAA TCCTTCCTCC TGTTGTTACA ATGCTCCATA TCTCTCCGTG 
TATAGTGAAA ACGCAGTTGA TATCTTTGAT GTAAACTCCA TGGAATGGAT ACAGACTCTT 
CCTCTCAAAA AGGTCCGACC TTTAAACACT GAAGGATCAT TAAATCTCTT AGGATTGGAG 
ACCATTAGAT TAATATATTT CAAAAATAAG ATGGCAGAAG GGGATGAACT TGTGGTTCCT 
GAAACATCAG ATAATAGTCG GAAACAAATG GTTAGAAATA TCAACAACAA ACGGCGTTAC 
TCCTTCCGAG TCCCTGAAGA AGAGAGGATG CAACAGAGGA GGGAGATGCT ACGAGATCCA 
GAAATGAGAA ATAAATTAAT TTCTAACCCA ACTAATTTTA ACCACATAGC ACACATGGGT 
CCTGGAGATG GAATACAGAT CCTAAAAGAC CTGCCCATGC CAGGTTTCCC TTATTCACCC 
CCTCATCACC ACTCCGGCCT GATCTCCTCT CCGAGCAACT TTGAGCACAT CTATCACATG 
ACTGTCACTT CTGCTGAACA GTTTCTCTCT CCTGAGTCCA TAAGCCCTGG GTCCCCCCCG 
TCCCTGCGCT CTGCCCCTGG CACACCACAC TCTGTGACTC TGAGGAACCC ACGGCCTCAG 
GAAAGCCGGA CAGTATTCAG TGGCTCCGTC AGTATTCCCT CCATCACCAA GTCCCGCCCT 
GAGCCAGGCC GCTCCATGAG TGCCAGCAGC GGCCTGTCTG CACGGTCGTC AGCCCAGAAT 
GGCAGTGCAC TAAAGAGGGA ATTCTCTGGA GGAAGCTATA ACACAAAGCG ACAGCCCATG 
CCCTCTCCCT CGGAGGGCTC TTTGTCATCA GGAGGCATGG ACCAAGGAAG TGACGCCCCT 
GCGAGGGACT ATGATGGAGA GGACTCTGAT TCTCCAAGGC ATTCCACAGC TTCCAACAGC 
TCTAACCTGA GCAGCCCCCC AAGTCCCATT TCACCCCAAA AGACCAAGAG CCTCTCCCTG 
GAGAGCACAG ACCGCGGGAG CTGGGATCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_006496801.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496864.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X2, mRNA.

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CloneID	OMu28721
Clone ID Related Accession (Same CDS sequence)	XM_017320119.2 , XM_017320119.1 , XM_006496801.3
Accession Version	XM_006496801.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496801.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAAGATTCC CTTTCTTCTT CCTCATCCTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC GTCCTGTACT CCAGCTAGCA AAGGCAGACG TATTGCAGAC 
TGTGCTCCAC TTCCAGCTCA CACACCAACC TTAAGGAAAA AGGGATGCCC GGCTTCAACT 
GGCTTTCCAC CTAAGCGCAA GACTCATCAA TTTTTCGTGA AGTCTTTCAC TGCCCCTACC 
AAGTGTCACC AGTGCACCTC CTTGATGGTC GGTTTGATAA GACAGGGCTG TTCCTGTGAA 
GTCTGTGGGT TCTCATGTCA TATAACTTGT GTGAACAAAG CCCCGACGGT TTGCCCAGTT 
CCTCCTGAGC AAACTAAAGG TCCACTGGGC ATAGACCCCC AGAAGGGAGT TGGGACGGCG 
TATGAGGGTC ATGTCAGGAT CCCTAAGCCG GCGGGAGTGA AGAAAGGGTG GCAGAGAGCA 
CTGGCTGTGG TCTGCGACTT CAAACTGTTT CTTTATGATA TCGCAGAGGG AAAAGCATCG 
CAGCCCACCT CCGTCATTAG TCAAGTGATT GACATGAGAG ATGAAGAATT TTCTGTGAGT 
TCAGTCTTAG CTTCTGATGT TATTCACGCA AGTCGAAAAG ATATCCCCTG TATATTCAGG 
GTCACAGCTT CCCAGCTCTC AGCACCTAGT AACAAATGTT CCATCCTGAT GCTAGCAGAC 
AGCGAGAATG AGAGGAGTAA GTGGGTGGGA GTGCTGAGTG AACTCCACAA GATTCTGAAG 
AAAAACAAGT TCAGAGACCG CTCAGTGTAT GTCCCCAAGG AGGCGTACGA CAGCACGCTA 
CCCCTCATTA AGACAACGCA GGCCGCTGCA ATCATAGATC ATGAACGGAT AGCTTTGGGA 
AATGAAGAAG GATTATTTGT TGTGCATGTC ACCAAAGATG AGATTGTTAG AGTTGGTGAT 
AATAAGAAAA TCCATCAGAT CGAACTCATT CCAAGTGACC AGCTTGTCGC TGTGATCTCA 
GGCCGAAATC GCCACGTCCG ACTTTTTCCT ATGTCAGCCT TGGATGGGCG AGAGACGGAT 
TTTTATAAAC TGGCAGAGAC TAAAGGGTGT CAGACCATAG CTGCTGGGAA AGTGCGCCAC 
GGAGCTCTGT CCTGCCTGTG TGTGGCCATG AAGAGACAGG TCCTCTGCTA TGAACTGTTC 
CAAAGCAAGA CCAGGCACAG GAAGTTTAAG GAGATTCAAG TCCCATGTAA TGTCCAGTGG 
ATGGCCATCT TCAGTGAGCA TCTCTGTGTG GGATTCCAGT CAGGATTTCT CAGATACCCC 
CTGAATGGAG AAGGAGGTCC ATGCAATATG CTCCATTCAA ATGACCATAC ACTGTCATTC 
ATTTCACACC AACCAATGGA CGCTCTCTGT GCAGTTGAGA TCTCCAACAA AGAGTACTTG 
CTGTGTTTTA ACAGCATTGG GATATACACA GACTGCCAGG GCCGAAGATC CAGACAGCAG 
GAACTGATGT GGCCAGCAAA TCCTTCCTCC TGTTGTTACA ATGCTCCATA TCTCTCCGTG 
TATAGTGAAA ACGCAGTTGA TATCTTTGAT GTAAACTCCA TGGAATGGAT ACAGACTCTT 
CCTCTCAAAA AGGTCCGACC TTTAAACACT GAAGGATCAT TAAATCTCTT AGGATTGGAG 
ACCATTAGAT TAATATATTT CAAAAATAAG ATGGCAGAAG GGGATGAACT TGTGGTTCCT 
GAAACATCAG ATAATAGTCG GAAACAAATG GTTAGAAATA TCAACAACAA ACGGCGTTAC 
TCCTTCCGAG TCCCTGAAGA AGAGAGGATG CAACAGAGGA GGGAGATGCT ACGAGATCCA 
GAAATGAGAA ATAAATTAAT TTCTAACCCA ACTAATTTTA ACCACATAGC ACACATGGGT 
CCTGGAGATG GAATACAGAT CCTAAAAGAC CTGCCCATGC CAGGTTTCCC TTATTCACCC 
CCTCATCACC ACTCCGGCCT GATCTCCTCT CCGAGCAACT TTGAGCACAT CTATCACATG 
ACTGTCACTT CTGCTGAACA GTTTCTCTCT CCTGAGTCCA TAAGCCCTGG GTCCCCCCCG 
TCCCTGCGCT CTGCCCCTGG CACACCACAC TCTGTGACTC TGAGGAACCC ACGGCCTCAG 
GAAAGCCGGA CAGTATTCAG TGGCTCCGTC AGTATTCCCT CCATCACCAA GTCCCGCCCT 
GAGCCAGGCC GCTCCATGAG TGCCAGCAGC GGCCTGTCTG CACGGTCGTC AGCCCAGAAT 
GGCAGTGCAC TAAAGAGGGA ATTCTCTGGA GGAAGCTATA ACACAAAGCG ACAGCCCATG 
CCCTCTCCCT CGGAGGGCTC TTTGTCATCA GGAGGCATGG ACCAAGGAAG TGACGCCCCT 
GCGAGGGACT ATGATGGAGA GGACTCTGAT TCTCCAAGGC ATTCCACAGC TTCCAACAGC 
TCTAACCTGA GCAGCCCCCC AAGTCCCATT TCACCCCAAA AGACCAAGAG CCTCTCCCTG 
GAGAGCACAG ACCGCGGGAG CTGGGATCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496864.1
CDS	675..6107
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSLSSSSSSLIDFLDDRSPSCTPASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006496801.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496801.3

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAAGATTCC CTTTCTTCTT CCTCATCCTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC GTCCTGTACT CCAGCTAGCA AAGGCAGACG TATTGCAGAC 
TGTGCTCCAC TTCCAGCTCA CACACCAACC TTAAGGAAAA AGGGATGCCC GGCTTCAACT 
GGCTTTCCAC CTAAGCGCAA GACTCATCAA TTTTTCGTGA AGTCTTTCAC TGCCCCTACC 
AAGTGTCACC AGTGCACCTC CTTGATGGTC GGTTTGATAA GACAGGGCTG TTCCTGTGAA 
GTCTGTGGGT TCTCATGTCA TATAACTTGT GTGAACAAAG CCCCGACGGT TTGCCCAGTT 
CCTCCTGAGC AAACTAAAGG TCCACTGGGC ATAGACCCCC AGAAGGGAGT TGGGACGGCG 
TATGAGGGTC ATGTCAGGAT CCCTAAGCCG GCGGGAGTGA AGAAAGGGTG GCAGAGAGCA 
CTGGCTGTGG TCTGCGACTT CAAACTGTTT CTTTATGATA TCGCAGAGGG AAAAGCATCG 
CAGCCCACCT CCGTCATTAG TCAAGTGATT GACATGAGAG ATGAAGAATT TTCTGTGAGT 
TCAGTCTTAG CTTCTGATGT TATTCACGCA AGTCGAAAAG ATATCCCCTG TATATTCAGG 
GTCACAGCTT CCCAGCTCTC AGCACCTAGT AACAAATGTT CCATCCTGAT GCTAGCAGAC 
AGCGAGAATG AGAGGAGTAA GTGGGTGGGA GTGCTGAGTG AACTCCACAA GATTCTGAAG 
AAAAACAAGT TCAGAGACCG CTCAGTGTAT GTCCCCAAGG AGGCGTACGA CAGCACGCTA 
CCCCTCATTA AGACAACGCA GGCCGCTGCA ATCATAGATC ATGAACGGAT AGCTTTGGGA 
AATGAAGAAG GATTATTTGT TGTGCATGTC ACCAAAGATG AGATTGTTAG AGTTGGTGAT 
AATAAGAAAA TCCATCAGAT CGAACTCATT CCAAGTGACC AGCTTGTCGC TGTGATCTCA 
GGCCGAAATC GCCACGTCCG ACTTTTTCCT ATGTCAGCCT TGGATGGGCG AGAGACGGAT 
TTTTATAAAC TGGCAGAGAC TAAAGGGTGT CAGACCATAG CTGCTGGGAA AGTGCGCCAC 
GGAGCTCTGT CCTGCCTGTG TGTGGCCATG AAGAGACAGG TCCTCTGCTA TGAACTGTTC 
CAAAGCAAGA CCAGGCACAG GAAGTTTAAG GAGATTCAAG TCCCATGTAA TGTCCAGTGG 
ATGGCCATCT TCAGTGAGCA TCTCTGTGTG GGATTCCAGT CAGGATTTCT CAGATACCCC 
CTGAATGGAG AAGGAGGTCC ATGCAATATG CTCCATTCAA ATGACCATAC ACTGTCATTC 
ATTTCACACC AACCAATGGA CGCTCTCTGT GCAGTTGAGA TCTCCAACAA AGAGTACTTG 
CTGTGTTTTA ACAGCATTGG GATATACACA GACTGCCAGG GCCGAAGATC CAGACAGCAG 
GAACTGATGT GGCCAGCAAA TCCTTCCTCC TGTTGTTACA ATGCTCCATA TCTCTCCGTG 
TATAGTGAAA ACGCAGTTGA TATCTTTGAT GTAAACTCCA TGGAATGGAT ACAGACTCTT 
CCTCTCAAAA AGGTCCGACC TTTAAACACT GAAGGATCAT TAAATCTCTT AGGATTGGAG 
ACCATTAGAT TAATATATTT CAAAAATAAG ATGGCAGAAG GGGATGAACT TGTGGTTCCT 
GAAACATCAG ATAATAGTCG GAAACAAATG GTTAGAAATA TCAACAACAA ACGGCGTTAC 
TCCTTCCGAG TCCCTGAAGA AGAGAGGATG CAACAGAGGA GGGAGATGCT ACGAGATCCA 
GAAATGAGAA ATAAATTAAT TTCTAACCCA ACTAATTTTA ACCACATAGC ACACATGGGT 
CCTGGAGATG GAATACAGAT CCTAAAAGAC CTGCCCATGC CAGGTTTCCC TTATTCACCC 
CCTCATCACC ACTCCGGCCT GATCTCCTCT CCGAGCAACT TTGAGCACAT CTATCACATG 
ACTGTCACTT CTGCTGAACA GTTTCTCTCT CCTGAGTCCA TAAGCCCTGG GTCCCCCCCG 
TCCCTGCGCT CTGCCCCTGG CACACCACAC TCTGTGACTC TGAGGAACCC ACGGCCTCAG 
GAAAGCCGGA CAGTATTCAG TGGCTCCGTC AGTATTCCCT CCATCACCAA GTCCCGCCCT 
GAGCCAGGCC GCTCCATGAG TGCCAGCAGC GGCCTGTCTG CACGGTCGTC AGCCCAGAAT 
GGCAGTGCAC TAAAGAGGGA ATTCTCTGGA GGAAGCTATA ACACAAAGCG ACAGCCCATG 
CCCTCTCCCT CGGAGGGCTC TTTGTCATCA GGAGGCATGG ACCAAGGAAG TGACGCCCCT 
GCGAGGGACT ATGATGGAGA GGACTCTGAT TCTCCAAGGC ATTCCACAGC TTCCAACAGC 
TCTAACCTGA GCAGCCCCCC AAGTCCCATT TCACCCCAAA AGACCAAGAG CCTCTCCCTG 
GAGAGCACAG ACCGCGGGAG CTGGGATCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_006496802.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496865.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X3, mRNA.

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CloneID	OMu28722
Clone ID Related Accession (Same CDS sequence)	XM_006496802.3 , XM_006496802.4
Accession Version	XM_006496802.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496802.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAAGATTCC CTTTCTTCTT CCTCATCCTC ACTGATTGAT 
TTTTTGGATG ACATTGCAGA CTGTGCTCCA CTTCCAGCTC ACACACCAAC CTTAAGGAAA 
AAGGGATGCC CGGCTTCAAC TGGCTTTCCA CCTAAGCGCA AGACTCATCA ATTTTTCGTG 
AAGTCTTTCA CTGCCCCTAC CAAGTGTCAC CAGTGCACCT CCTTGATGGT CGGTTTGATA 
AGACAGGGCT GTTCCTGTGA AGTCTGTGGG TTCTCATGTC ATATAACTTG TGTGAACAAA 
GCCCCGACGG TTTGCCCAGT TCCTCCTGAG CAAACTAAAG GTCCACTGGG CATAGACCCC 
CAGAAGGGAG TTGGGACGGC GTATGAGGGT CATGTCAGGA TCCCTAAGCC GGCGGGAGTG 
AAGAAAGGGT GGCAGAGAGC ACTGGCTGTG GTCTGCGACT TCAAACTGTT TCTTTATGAT 
ATCGCAGAGG GAAAAGCATC GCAGCCCACC TCCGTCATTA GTCAAGTGAT TGACATGAGA 
GATGAAGAAT TTTCTGTGAG TTCAGTCTTA GCTTCTGATG TTATTCACGC AAGTCGAAAA 
GATATCCCCT GTATATTCAG GGTCACAGCT TCCCAGCTCT CAGCACCTAG TAACAAATGT 
TCCATCCTGA TGCTAGCAGA CAGCGAGAAT GAGAGGAGTA AGTGGGTGGG AGTGCTGAGT 
GAACTCCACA AGATTCTGAA GAAAAACAAG TTCAGAGACC GCTCAGTGTA TGTCCCCAAG 
GAGGCGTACG ACAGCACGCT ACCCCTCATT AAGACAACGC AGGCCGCTGC AATCATAGAT 
CATGAACGGA TAGCTTTGGG AAATGAAGAA GGATTATTTG TTGTGCATGT CACCAAAGAT 
GAGATTGTTA GAGTTGGTGA TAATAAGAAA ATCCATCAGA TCGAACTCAT TCCAAGTGAC 
CAGCTTGTCG CTGTGATCTC AGGCCGAAAT CGCCACGTCC GACTTTTTCC TATGTCAGCC 
TTGGATGGGC GAGAGACGGA TTTTTATAAA CTGGCAGAGA CTAAAGGGTG TCAGACCATA 
GCTGCTGGGA AAGTGCGCCA CGGAGCTCTG TCCTGCCTGT GTGTGGCCAT GAAGAGACAG 
GTCCTCTGCT ATGAACTGTT CCAAAGCAAG ACCAGGCACA GGAAGTTTAA GGAGATTCAA 
GTCCCATGTA ATGTCCAGTG GATGGCCATC TTCAGTGAGC ATCTCTGTGT GGGATTCCAG 
TCAGGATTTC TCAGATACCC CCTGAATGGA GAAGGAGGTC CATGCAATAT GCTCCATTCA 
AATGACCATA CACTGTCATT CATTTCACAC CAACCAATGG ACGCTCTCTG TGCAGTTGAG 
ATCTCCAACA AAGAGTACTT GCTGTGTTTT AACAGCATTG GGATATACAC AGACTGCCAG 
GGCCGAAGAT CCAGACAGCA GGAACTGATG TGGCCAGCAA ATCCTTCCTC CTGTTGTTAC 
AATGCTCCAT ATCTCTCCGT GTATAGTGAA AACGCAGTTG ATATCTTTGA TGTAAACTCC 
ATGGAATGGA TACAGACTCT TCCTCTCAAA AAGGTCCGAC CTTTAAACAC TGAAGGATCA 
TTAAATCTCT TAGGATTGGA GACCATTAGA TTAATATATT TCAAAAATAA GATGGCAGAA 
GGGGATGAAC TTGTGGTTCC TGAAACATCA GATAATAGTC GGAAACAAAT GGTTAGAAAT 
ATCAACAACA AACGGCGTTA CTCCTTCCGA GTCCCTGAAG AAGAGAGGAT GCAACAGAGG 
AGGGAGATGC TACGAGATCC AGAAATGAGA AATAAATTAA TTTCTAACCC AACTAATTTT 
AACCACATAG CACACATGGG TCCTGGAGAT GGAATACAGA TCCTAAAAGA CCTGCCCATG 
CCAGGTTTCC CTTATTCACC CCCTCATCAC CACTCCGGCC TGATCTCCTC TCCGAGCAAC 
TTTGAGCACA TCTATCACAT GACTGTCACT TCTGCTGAAC AGTTTCTCTC TCCTGAGTCC 
ATAAGCCCTG GGTCCCCCCC GTCCCTGCGC TCTGCCCCTG GCACACCACA CTCTGTGACT 
CTGAGGAACC CACGGCCTCA GGAAAGCCGG ACAGTATTCA GTGGCTCCGT CAGTATTCCC 
TCCATCACCA AGTCCCGCCC TGAGCCAGGC CGCTCCATGA GTGCCAGCAG CGGCCTGTCT 
GCACGGTCGT CAGCCCAGAA TGGCAGTGCA CTAAAGAGGG AATTCTCTGG AGGAAGCTAT 
AACACAAAGC GACAGCCCAT GCCCTCTCCC TCGGAGGGCT CTTTGTCATC AGGAGGCATG 
GACCAAGGAA GTGACGCCCC TGCGAGGGAC TATGATGGAG AGGACTCTGA TTCTCCAAGG 
CATTCCACAG CTTCCAACAG CTCTAACCTG AGCAGCCCCC CAAGTCCCAT TTCACCCCAA 
AAGACCAAGA GCCTCTCCCT GGAGAGCACA GACCGCGGGA GCTGGGATCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496865.1
CDS	1142..6535
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSLSSSSSSLIDFLDDIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006496802.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496802.3

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAAGATTCC CTTTCTTCTT CCTCATCCTC ACTGATTGAT 
TTTTTGGATG ACATTGCAGA CTGTGCTCCA CTTCCAGCTC ACACACCAAC CTTAAGGAAA 
AAGGGATGCC CGGCTTCAAC TGGCTTTCCA CCTAAGCGCA AGACTCATCA ATTTTTCGTG 
AAGTCTTTCA CTGCCCCTAC CAAGTGTCAC CAGTGCACCT CCTTGATGGT CGGTTTGATA 
AGACAGGGCT GTTCCTGTGA AGTCTGTGGG TTCTCATGTC ATATAACTTG TGTGAACAAA 
GCCCCGACGG TTTGCCCAGT TCCTCCTGAG CAAACTAAAG GTCCACTGGG CATAGACCCC 
CAGAAGGGAG TTGGGACGGC GTATGAGGGT CATGTCAGGA TCCCTAAGCC GGCGGGAGTG 
AAGAAAGGGT GGCAGAGAGC ACTGGCTGTG GTCTGCGACT TCAAACTGTT TCTTTATGAT 
ATCGCAGAGG GAAAAGCATC GCAGCCCACC TCCGTCATTA GTCAAGTGAT TGACATGAGA 
GATGAAGAAT TTTCTGTGAG TTCAGTCTTA GCTTCTGATG TTATTCACGC AAGTCGAAAA 
GATATCCCCT GTATATTCAG GGTCACAGCT TCCCAGCTCT CAGCACCTAG TAACAAATGT 
TCCATCCTGA TGCTAGCAGA CAGCGAGAAT GAGAGGAGTA AGTGGGTGGG AGTGCTGAGT 
GAACTCCACA AGATTCTGAA GAAAAACAAG TTCAGAGACC GCTCAGTGTA TGTCCCCAAG 
GAGGCGTACG ACAGCACGCT ACCCCTCATT AAGACAACGC AGGCCGCTGC AATCATAGAT 
CATGAACGGA TAGCTTTGGG AAATGAAGAA GGATTATTTG TTGTGCATGT CACCAAAGAT 
GAGATTGTTA GAGTTGGTGA TAATAAGAAA ATCCATCAGA TCGAACTCAT TCCAAGTGAC 
CAGCTTGTCG CTGTGATCTC AGGCCGAAAT CGCCACGTCC GACTTTTTCC TATGTCAGCC 
TTGGATGGGC GAGAGACGGA TTTTTATAAA CTGGCAGAGA CTAAAGGGTG TCAGACCATA 
GCTGCTGGGA AAGTGCGCCA CGGAGCTCTG TCCTGCCTGT GTGTGGCCAT GAAGAGACAG 
GTCCTCTGCT ATGAACTGTT CCAAAGCAAG ACCAGGCACA GGAAGTTTAA GGAGATTCAA 
GTCCCATGTA ATGTCCAGTG GATGGCCATC TTCAGTGAGC ATCTCTGTGT GGGATTCCAG 
TCAGGATTTC TCAGATACCC CCTGAATGGA GAAGGAGGTC CATGCAATAT GCTCCATTCA 
AATGACCATA CACTGTCATT CATTTCACAC CAACCAATGG ACGCTCTCTG TGCAGTTGAG 
ATCTCCAACA AAGAGTACTT GCTGTGTTTT AACAGCATTG GGATATACAC AGACTGCCAG 
GGCCGAAGAT CCAGACAGCA GGAACTGATG TGGCCAGCAA ATCCTTCCTC CTGTTGTTAC 
AATGCTCCAT ATCTCTCCGT GTATAGTGAA AACGCAGTTG ATATCTTTGA TGTAAACTCC 
ATGGAATGGA TACAGACTCT TCCTCTCAAA AAGGTCCGAC CTTTAAACAC TGAAGGATCA 
TTAAATCTCT TAGGATTGGA GACCATTAGA TTAATATATT TCAAAAATAA GATGGCAGAA 
GGGGATGAAC TTGTGGTTCC TGAAACATCA GATAATAGTC GGAAACAAAT GGTTAGAAAT 
ATCAACAACA AACGGCGTTA CTCCTTCCGA GTCCCTGAAG AAGAGAGGAT GCAACAGAGG 
AGGGAGATGC TACGAGATCC AGAAATGAGA AATAAATTAA TTTCTAACCC AACTAATTTT 
AACCACATAG CACACATGGG TCCTGGAGAT GGAATACAGA TCCTAAAAGA CCTGCCCATG 
CCAGGTTTCC CTTATTCACC CCCTCATCAC CACTCCGGCC TGATCTCCTC TCCGAGCAAC 
TTTGAGCACA TCTATCACAT GACTGTCACT TCTGCTGAAC AGTTTCTCTC TCCTGAGTCC 
ATAAGCCCTG GGTCCCCCCC GTCCCTGCGC TCTGCCCCTG GCACACCACA CTCTGTGACT 
CTGAGGAACC CACGGCCTCA GGAAAGCCGG ACAGTATTCA GTGGCTCCGT CAGTATTCCC 
TCCATCACCA AGTCCCGCCC TGAGCCAGGC CGCTCCATGA GTGCCAGCAG CGGCCTGTCT 
GCACGGTCGT CAGCCCAGAA TGGCAGTGCA CTAAAGAGGG AATTCTCTGG AGGAAGCTAT 
AACACAAAGC GACAGCCCAT GCCCTCTCCC TCGGAGGGCT CTTTGTCATC AGGAGGCATG 
GACCAAGGAA GTGACGCCCC TGCGAGGGAC TATGATGGAG AGGACTCTGA TTCTCCAAGG 
CATTCCACAG CTTCCAACAG CTCTAACCTG AGCAGCCCCC CAAGTCCCAT TTCACCCCAA 
AAGACCAAGA GCCTCTCCCT GGAGAGCACA GACCGCGGGA GCTGGGATCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_006496803.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496866.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X4, mRNA.

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CloneID	OMu28723
Clone ID Related Accession (Same CDS sequence)	XM_006496803.4 , XM_006496803.3
Accession Version	XM_006496803.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496803.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAACGCTCT CCGTCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTATTGCAG ACTGTGCTCC ACTTCCAGCT CACACACCAA CCTTAAGGAA AAAGGGATGC 
CCGGCTTCAA CTGGCTTTCC ACCTAAGCGC AAGACTCATC AATTTTTCGT GAAGTCTTTC 
ACTGCCCCTA CCAAGTGTCA CCAGTGCACC TCCTTGATGG TCGGTTTGAT AAGACAGGGC 
TGTTCCTGTG AAGTCTGTGG GTTCTCATGT CATATAACTT GTGTGAACAA AGCCCCGACG 
GTTTGCCCAG TTCCTCCTGA GCAAACTAAA GGTCCACTGG GCATAGACCC CCAGAAGGGA 
GTTGGGACGG CGTATGAGGG TCATGTCAGG ATCCCTAAGC CGGCGGGAGT GAAGAAAGGG 
TGGCAGAGAG CACTGGCTGT GGTCTGCGAC TTCAAACTGT TTCTTTATGA TATCGCAGAG 
GGAAAAGCAT CGCAGCCCAC CTCCGTCATT AGTCAAGTGA TTGACATGAG AGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT AGCTTCTGAT GTTATTCACG CAAGTCGAAA AGATATCCCC 
TGTATATTCA GGGTCACAGC TTCCCAGCTC TCAGCACCTA GTAACAAATG TTCCATCCTG 
ATGCTAGCAG ACAGCGAGAA TGAGAGGAGT AAGTGGGTGG GAGTGCTGAG TGAACTCCAC 
AAGATTCTGA AGAAAAACAA GTTCAGAGAC CGCTCAGTGT ATGTCCCCAA GGAGGCGTAC 
GACAGCACGC TACCCCTCAT TAAGACAACG CAGGCCGCTG CAATCATAGA TCATGAACGG 
ATAGCTTTGG GAAATGAAGA AGGATTATTT GTTGTGCATG TCACCAAAGA TGAGATTGTT 
AGAGTTGGTG ATAATAAGAA AATCCATCAG ATCGAACTCA TTCCAAGTGA CCAGCTTGTC 
GCTGTGATCT CAGGCCGAAA TCGCCACGTC CGACTTTTTC CTATGTCAGC CTTGGATGGG 
CGAGAGACGG ATTTTTATAA ACTGGCAGAG ACTAAAGGGT GTCAGACCAT AGCTGCTGGG 
AAAGTGCGCC ACGGAGCTCT GTCCTGCCTG TGTGTGGCCA TGAAGAGACA GGTCCTCTGC 
TATGAACTGT TCCAAAGCAA GACCAGGCAC AGGAAGTTTA AGGAGATTCA AGTCCCATGT 
AATGTCCAGT GGATGGCCAT CTTCAGTGAG CATCTCTGTG TGGGATTCCA GTCAGGATTT 
CTCAGATACC CCCTGAATGG AGAAGGAGGT CCATGCAATA TGCTCCATTC AAATGACCAT 
ACACTGTCAT TCATTTCACA CCAACCAATG GACGCTCTCT GTGCAGTTGA GATCTCCAAC 
AAAGAGTACT TGCTGTGTTT TAACAGCATT GGGATATACA CAGACTGCCA GGGCCGAAGA 
TCCAGACAGC AGGAACTGAT GTGGCCAGCA AATCCTTCCT CCTGTTGTTA CAATGCTCCA 
TATCTCTCCG TGTATAGTGA AAACGCAGTT GATATCTTTG ATGTAAACTC CATGGAATGG 
ATACAGACTC TTCCTCTCAA AAAGGTCCGA CCTTTAAACA CTGAAGGATC ATTAAATCTC 
TTAGGATTGG AGACCATTAG ATTAATATAT TTCAAAAATA AGATGGCAGA AGGGGATGAA 
CTTGTGGTTC CTGAAACATC AGATAATAGT CGGAAACAAA TGGTTAGAAA TATCAACAAC 
AAACGGCGTT ACTCCTTCCG AGTCCCTGAA GAAGAGAGGA TGCAACAGAG GAGGGAGATG 
CTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAACC CAACTAATTT TAACCACATA 
GCACACATGG GTCCTGGAGA TGGAATACAG ATCCTAAAAG ACCTGCCCAT GCCAGGTTTC 
CCTTATTCAC CCCCTCATCA CCACTCCGGC CTGATCTCCT CTCCGAGCAA CTTTGAGCAC 
ATCTATCACA TGACTGTCAC TTCTGCTGAA CAGTTTCTCT CTCCTGAGTC CATAAGCCCT 
GGGTCCCCCC CGTCCCTGCG CTCTGCCCCT GGCACACCAC ACTCTGTGAC TCTGAGGAAC 
CCACGGCCTC AGGAAAGCCG GACAGTATTC AGTGGCTCCG TCAGTATTCC CTCCATCACC 
AAGTCCCGCC CTGAGCCAGG CCGCTCCATG AGTGCCAGCA GCGGCCTGTC TGCACGGTCG 
TCAGCCCAGA ATGGCAGTGC ACTAAAGAGG GAATTCTCTG GAGGAAGCTA TAACACAAAG 
CGACAGCCCA TGCCCTCTCC CTCGGAGGGC TCTTTGTCAT CAGGAGGCAT GGACCAAGGA 
AGTGACGCCC CTGCGAGGGA CTATGATGGA GAGGACTCTG ATTCTCCAAG GCATTCCACA 
GCTTCCAACA GCTCTAACCT GAGCAGCCCC CCAAGTCCCA TTTCACCCCA AAAGACCAAG 
AGCCTCTCCC TGGAGAGCAC AGACCGCGGG AGCTGGGATC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496866.1
CDS	1142..6526
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006496803.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496803.3

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAACGCTCT CCGTCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTATTGCAG ACTGTGCTCC ACTTCCAGCT CACACACCAA CCTTAAGGAA AAAGGGATGC 
CCGGCTTCAA CTGGCTTTCC ACCTAAGCGC AAGACTCATC AATTTTTCGT GAAGTCTTTC 
ACTGCCCCTA CCAAGTGTCA CCAGTGCACC TCCTTGATGG TCGGTTTGAT AAGACAGGGC 
TGTTCCTGTG AAGTCTGTGG GTTCTCATGT CATATAACTT GTGTGAACAA AGCCCCGACG 
GTTTGCCCAG TTCCTCCTGA GCAAACTAAA GGTCCACTGG GCATAGACCC CCAGAAGGGA 
GTTGGGACGG CGTATGAGGG TCATGTCAGG ATCCCTAAGC CGGCGGGAGT GAAGAAAGGG 
TGGCAGAGAG CACTGGCTGT GGTCTGCGAC TTCAAACTGT TTCTTTATGA TATCGCAGAG 
GGAAAAGCAT CGCAGCCCAC CTCCGTCATT AGTCAAGTGA TTGACATGAG AGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT AGCTTCTGAT GTTATTCACG CAAGTCGAAA AGATATCCCC 
TGTATATTCA GGGTCACAGC TTCCCAGCTC TCAGCACCTA GTAACAAATG TTCCATCCTG 
ATGCTAGCAG ACAGCGAGAA TGAGAGGAGT AAGTGGGTGG GAGTGCTGAG TGAACTCCAC 
AAGATTCTGA AGAAAAACAA GTTCAGAGAC CGCTCAGTGT ATGTCCCCAA GGAGGCGTAC 
GACAGCACGC TACCCCTCAT TAAGACAACG CAGGCCGCTG CAATCATAGA TCATGAACGG 
ATAGCTTTGG GAAATGAAGA AGGATTATTT GTTGTGCATG TCACCAAAGA TGAGATTGTT 
AGAGTTGGTG ATAATAAGAA AATCCATCAG ATCGAACTCA TTCCAAGTGA CCAGCTTGTC 
GCTGTGATCT CAGGCCGAAA TCGCCACGTC CGACTTTTTC CTATGTCAGC CTTGGATGGG 
CGAGAGACGG ATTTTTATAA ACTGGCAGAG ACTAAAGGGT GTCAGACCAT AGCTGCTGGG 
AAAGTGCGCC ACGGAGCTCT GTCCTGCCTG TGTGTGGCCA TGAAGAGACA GGTCCTCTGC 
TATGAACTGT TCCAAAGCAA GACCAGGCAC AGGAAGTTTA AGGAGATTCA AGTCCCATGT 
AATGTCCAGT GGATGGCCAT CTTCAGTGAG CATCTCTGTG TGGGATTCCA GTCAGGATTT 
CTCAGATACC CCCTGAATGG AGAAGGAGGT CCATGCAATA TGCTCCATTC AAATGACCAT 
ACACTGTCAT TCATTTCACA CCAACCAATG GACGCTCTCT GTGCAGTTGA GATCTCCAAC 
AAAGAGTACT TGCTGTGTTT TAACAGCATT GGGATATACA CAGACTGCCA GGGCCGAAGA 
TCCAGACAGC AGGAACTGAT GTGGCCAGCA AATCCTTCCT CCTGTTGTTA CAATGCTCCA 
TATCTCTCCG TGTATAGTGA AAACGCAGTT GATATCTTTG ATGTAAACTC CATGGAATGG 
ATACAGACTC TTCCTCTCAA AAAGGTCCGA CCTTTAAACA CTGAAGGATC ATTAAATCTC 
TTAGGATTGG AGACCATTAG ATTAATATAT TTCAAAAATA AGATGGCAGA AGGGGATGAA 
CTTGTGGTTC CTGAAACATC AGATAATAGT CGGAAACAAA TGGTTAGAAA TATCAACAAC 
AAACGGCGTT ACTCCTTCCG AGTCCCTGAA GAAGAGAGGA TGCAACAGAG GAGGGAGATG 
CTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAACC CAACTAATTT TAACCACATA 
GCACACATGG GTCCTGGAGA TGGAATACAG ATCCTAAAAG ACCTGCCCAT GCCAGGTTTC 
CCTTATTCAC CCCCTCATCA CCACTCCGGC CTGATCTCCT CTCCGAGCAA CTTTGAGCAC 
ATCTATCACA TGACTGTCAC TTCTGCTGAA CAGTTTCTCT CTCCTGAGTC CATAAGCCCT 
GGGTCCCCCC CGTCCCTGCG CTCTGCCCCT GGCACACCAC ACTCTGTGAC TCTGAGGAAC 
CCACGGCCTC AGGAAAGCCG GACAGTATTC AGTGGCTCCG TCAGTATTCC CTCCATCACC 
AAGTCCCGCC CTGAGCCAGG CCGCTCCATG AGTGCCAGCA GCGGCCTGTC TGCACGGTCG 
TCAGCCCAGA ATGGCAGTGC ACTAAAGAGG GAATTCTCTG GAGGAAGCTA TAACACAAAG 
CGACAGCCCA TGCCCTCTCC CTCGGAGGGC TCTTTGTCAT CAGGAGGCAT GGACCAAGGA 
AGTGACGCCC CTGCGAGGGA CTATGATGGA GAGGACTCTG ATTCTCCAAG GCATTCCACA 
GCTTCCAACA GCTCTAACCT GAGCAGCCCC CCAAGTCCCA TTTCACCCCA AAAGACCAAG 
AGCCTCTCCC TGGAGAGCAC AGACCGCGGG AGCTGGGATC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_006496804.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496867.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X5, mRNA.

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CloneID	OMu28724
Clone ID Related Accession (Same CDS sequence)	XM_006496804.3 , XM_006496804.4
Accession Version	XM_006496804.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496804.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGATT CCCTTTCTTC TTCCTCATCC TCACTGATTG ATTTTTTGGA TGACCGCTCT 
CCGTCCTGTA CTCCAGCTAG CAAAGGCAGA CGTATTGCAG ACTGTGCTCC ACTTCCAGCT 
CACACACCAA CCTTAAGGAA AAAGGGATGC CCGGCTTCAA CTGGCTTTCC ACCTAAGCGC 
AAGACTCATC AATTTTTCGT GAAGTCTTTC ACTGCCCCTA CCAAGTGTCA CCAGTGCACC 
TCCTTGATGG TCGGTTTGAT AAGACAGGGC TGTTCCTGTG AAGTCTGTGG GTTCTCATGT 
CATATAACTT GTGTGAACAA AGCCCCGACG GTTTGCCCAG TTCCTCCTGA GCAAACTAAA 
GGTCCACTGG GCATAGACCC CCAGAAGGGA GTTGGGACGG CGTATGAGGG TCATGTCAGG 
ATCCCTAAGC CGGCGGGAGT GAAGAAAGGG TGGCAGAGAG CACTGGCTGT GGTCTGCGAC 
TTCAAACTGT TTCTTTATGA TATCGCAGAG GGAAAAGCAT CGCAGCCCAC CTCCGTCATT 
AGTCAAGTGA TTGACATGAG AGATGAAGAA TTTTCTGTGA GTTCAGTCTT AGCTTCTGAT 
GTTATTCACG CAAGTCGAAA AGATATCCCC TGTATATTCA GGGTCACAGC TTCCCAGCTC 
TCAGCACCTA GTAACAAATG TTCCATCCTG ATGCTAGCAG ACAGCGAGAA TGAGAGGAGT 
AAGTGGGTGG GAGTGCTGAG TGAACTCCAC AAGATTCTGA AGAAAAACAA GTTCAGAGAC 
CGCTCAGTGT ATGTCCCCAA GGAGGCGTAC GACAGCACGC TACCCCTCAT TAAGACAACG 
CAGGCCGCTG CAATCATAGA TCATGAACGG ATAGCTTTGG GAAATGAAGA AGGATTATTT 
GTTGTGCATG TCACCAAAGA TGAGATTGTT AGAGTTGGTG ATAATAAGAA AATCCATCAG 
ATCGAACTCA TTCCAAGTGA CCAGCTTGTC GCTGTGATCT CAGGCCGAAA TCGCCACGTC 
CGACTTTTTC CTATGTCAGC CTTGGATGGG CGAGAGACGG ATTTTTATAA ACTGGCAGAG 
ACTAAAGGGT GTCAGACCAT AGCTGCTGGG AAAGTGCGCC ACGGAGCTCT GTCCTGCCTG 
TGTGTGGCCA TGAAGAGACA GGTCCTCTGC TATGAACTGT TCCAAAGCAA GACCAGGCAC 
AGGAAGTTTA AGGAGATTCA AGTCCCATGT AATGTCCAGT GGATGGCCAT CTTCAGTGAG 
CATCTCTGTG TGGGATTCCA GTCAGGATTT CTCAGATACC CCCTGAATGG AGAAGGAGGT 
CCATGCAATA TGCTCCATTC AAATGACCAT ACACTGTCAT TCATTTCACA CCAACCAATG 
GACGCTCTCT GTGCAGTTGA GATCTCCAAC AAAGAGTACT TGCTGTGTTT TAACAGCATT 
GGGATATACA CAGACTGCCA GGGCCGAAGA TCCAGACAGC AGGAACTGAT GTGGCCAGCA 
AATCCTTCCT CCTGTTGTTA CAATGCTCCA TATCTCTCCG TGTATAGTGA AAACGCAGTT 
GATATCTTTG ATGTAAACTC CATGGAATGG ATACAGACTC TTCCTCTCAA AAAGGTCCGA 
CCTTTAAACA CTGAAGGATC ATTAAATCTC TTAGGATTGG AGACCATTAG ATTAATATAT 
TTCAAAAATA AGATGGCAGA AGGGGATGAA CTTGTGGTTC CTGAAACATC AGATAATAGT 
CGGAAACAAA TGGTTAGAAA TATCAACAAC AAACGGCGTT ACTCCTTCCG AGTCCCTGAA 
GAAGAGAGGA TGCAACAGAG GAGGGAGATG CTACGAGATC CAGAAATGAG AAATAAATTA 
ATTTCTAACC CAACTAATTT TAACCACATA GCACACATGG GTCCTGGAGA TGGAATACAG 
ATCCTAAAAG ACCTGCCCAT GCCAGGTTTC CCTTATTCAC CCCCTCATCA CCACTCCGGC 
CTGATCTCCT CTCCGAGCAA CTTTGAGCAC ATCTATCACA TGACTGTCAC TTCTGCTGAA 
CAGTTTCTCT CTCCTGAGTC CATAAGCCCT GGGTCCCCCC CGTCCCTGCG CTCTGCCCCT 
GGCACACCAC ACTCTGTGAC TCTGAGGAAC CCACGGCCTC AGGAAAGCCG GACAGTATTC 
AGTGGCTCCG TCAGTATTCC CTCCATCACC AAGTCCCGCC CTGAGCCAGG CCGCTCCATG 
AGTGCCAGCA GCGGCCTGTC TGCACGGTCG TCAGCCCAGA ATGGCAGTGC ACTAAAGAGG 
GAATTCTCTG GAGGAAGCTA TAACACAAAG CGACAGCCCA TGCCCTCTCC CTCGGAGGGC 
TCTTTGTCAT CAGGAGGCAT GGACCAAGGA AGTGACGCCC CTGCGAGGGA CTATGATGGA 
GAGGACTCTG ATTCTCCAAG GCATTCCACA GCTTCCAACA GCTCTAACCT GAGCAGCCCC 
CCAAGTCCCA TTTCACCCCA AAAGACCAAG AGCCTCTCCC TGGAGAGCAC AGACCGCGGG 
AGCTGGGATC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496867.1
CDS	1142..6496
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKDSLSSSSSSLIDFLDDRSPSCTPASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006496804.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496804.3

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGATT CCCTTTCTTC TTCCTCATCC TCACTGATTG ATTTTTTGGA TGACCGCTCT 
CCGTCCTGTA CTCCAGCTAG CAAAGGCAGA CGTATTGCAG ACTGTGCTCC ACTTCCAGCT 
CACACACCAA CCTTAAGGAA AAAGGGATGC CCGGCTTCAA CTGGCTTTCC ACCTAAGCGC 
AAGACTCATC AATTTTTCGT GAAGTCTTTC ACTGCCCCTA CCAAGTGTCA CCAGTGCACC 
TCCTTGATGG TCGGTTTGAT AAGACAGGGC TGTTCCTGTG AAGTCTGTGG GTTCTCATGT 
CATATAACTT GTGTGAACAA AGCCCCGACG GTTTGCCCAG TTCCTCCTGA GCAAACTAAA 
GGTCCACTGG GCATAGACCC CCAGAAGGGA GTTGGGACGG CGTATGAGGG TCATGTCAGG 
ATCCCTAAGC CGGCGGGAGT GAAGAAAGGG TGGCAGAGAG CACTGGCTGT GGTCTGCGAC 
TTCAAACTGT TTCTTTATGA TATCGCAGAG GGAAAAGCAT CGCAGCCCAC CTCCGTCATT 
AGTCAAGTGA TTGACATGAG AGATGAAGAA TTTTCTGTGA GTTCAGTCTT AGCTTCTGAT 
GTTATTCACG CAAGTCGAAA AGATATCCCC TGTATATTCA GGGTCACAGC TTCCCAGCTC 
TCAGCACCTA GTAACAAATG TTCCATCCTG ATGCTAGCAG ACAGCGAGAA TGAGAGGAGT 
AAGTGGGTGG GAGTGCTGAG TGAACTCCAC AAGATTCTGA AGAAAAACAA GTTCAGAGAC 
CGCTCAGTGT ATGTCCCCAA GGAGGCGTAC GACAGCACGC TACCCCTCAT TAAGACAACG 
CAGGCCGCTG CAATCATAGA TCATGAACGG ATAGCTTTGG GAAATGAAGA AGGATTATTT 
GTTGTGCATG TCACCAAAGA TGAGATTGTT AGAGTTGGTG ATAATAAGAA AATCCATCAG 
ATCGAACTCA TTCCAAGTGA CCAGCTTGTC GCTGTGATCT CAGGCCGAAA TCGCCACGTC 
CGACTTTTTC CTATGTCAGC CTTGGATGGG CGAGAGACGG ATTTTTATAA ACTGGCAGAG 
ACTAAAGGGT GTCAGACCAT AGCTGCTGGG AAAGTGCGCC ACGGAGCTCT GTCCTGCCTG 
TGTGTGGCCA TGAAGAGACA GGTCCTCTGC TATGAACTGT TCCAAAGCAA GACCAGGCAC 
AGGAAGTTTA AGGAGATTCA AGTCCCATGT AATGTCCAGT GGATGGCCAT CTTCAGTGAG 
CATCTCTGTG TGGGATTCCA GTCAGGATTT CTCAGATACC CCCTGAATGG AGAAGGAGGT 
CCATGCAATA TGCTCCATTC AAATGACCAT ACACTGTCAT TCATTTCACA CCAACCAATG 
GACGCTCTCT GTGCAGTTGA GATCTCCAAC AAAGAGTACT TGCTGTGTTT TAACAGCATT 
GGGATATACA CAGACTGCCA GGGCCGAAGA TCCAGACAGC AGGAACTGAT GTGGCCAGCA 
AATCCTTCCT CCTGTTGTTA CAATGCTCCA TATCTCTCCG TGTATAGTGA AAACGCAGTT 
GATATCTTTG ATGTAAACTC CATGGAATGG ATACAGACTC TTCCTCTCAA AAAGGTCCGA 
CCTTTAAACA CTGAAGGATC ATTAAATCTC TTAGGATTGG AGACCATTAG ATTAATATAT 
TTCAAAAATA AGATGGCAGA AGGGGATGAA CTTGTGGTTC CTGAAACATC AGATAATAGT 
CGGAAACAAA TGGTTAGAAA TATCAACAAC AAACGGCGTT ACTCCTTCCG AGTCCCTGAA 
GAAGAGAGGA TGCAACAGAG GAGGGAGATG CTACGAGATC CAGAAATGAG AAATAAATTA 
ATTTCTAACC CAACTAATTT TAACCACATA GCACACATGG GTCCTGGAGA TGGAATACAG 
ATCCTAAAAG ACCTGCCCAT GCCAGGTTTC CCTTATTCAC CCCCTCATCA CCACTCCGGC 
CTGATCTCCT CTCCGAGCAA CTTTGAGCAC ATCTATCACA TGACTGTCAC TTCTGCTGAA 
CAGTTTCTCT CTCCTGAGTC CATAAGCCCT GGGTCCCCCC CGTCCCTGCG CTCTGCCCCT 
GGCACACCAC ACTCTGTGAC TCTGAGGAAC CCACGGCCTC AGGAAAGCCG GACAGTATTC 
AGTGGCTCCG TCAGTATTCC CTCCATCACC AAGTCCCGCC CTGAGCCAGG CCGCTCCATG 
AGTGCCAGCA GCGGCCTGTC TGCACGGTCG TCAGCCCAGA ATGGCAGTGC ACTAAAGAGG 
GAATTCTCTG GAGGAAGCTA TAACACAAAG CGACAGCCCA TGCCCTCTCC CTCGGAGGGC 
TCTTTGTCAT CAGGAGGCAT GGACCAAGGA AGTGACGCCC CTGCGAGGGA CTATGATGGA 
GAGGACTCTG ATTCTCCAAG GCATTCCACA GCTTCCAACA GCTCTAACCT GAGCAGCCCC 
CCAAGTCCCA TTTCACCCCA AAAGACCAAG AGCCTCTCCC TGGAGAGCAC AGACCGCGGG 
AGCTGGGATC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_006496805.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496868.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X6, mRNA.

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CloneID	OMu28725
Clone ID Related Accession (Same CDS sequence)	NM_001359541.1 , XM_006496805.3
Accession Version	XM_006496805.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496805.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAAATTGCA GACTGTGCTC CACTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG CCCGGCTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAT 
CAATTTTTCG TGAAGTCTTT CACTGCCCCT ACCAAGTGTC ACCAGTGCAC CTCCTTGATG 
GTCGGTTTGA TAAGACAGGG CTGTTCCTGT GAAGTCTGTG GGTTCTCATG TCATATAACT 
TGTGTGAACA AAGCCCCGAC GGTTTGCCCA GTTCCTCCTG AGCAAACTAA AGGTCCACTG 
GGCATAGACC CCCAGAAGGG AGTTGGGACG GCGTATGAGG GTCATGTCAG GATCCCTAAG 
CCGGCGGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTG TGGTCTGCGA CTTCAAACTG 
TTTCTTTATG ATATCGCAGA GGGAAAAGCA TCGCAGCCCA CCTCCGTCAT TAGTCAAGTG 
ATTGACATGA GAGATGAAGA ATTTTCTGTG AGTTCAGTCT TAGCTTCTGA TGTTATTCAC 
GCAAGTCGAA AAGATATCCC CTGTATATTC AGGGTCACAG CTTCCCAGCT CTCAGCACCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCA GACAGCGAGA ATGAGAGGAG TAAGTGGGTG 
GGAGTGCTGA GTGAACTCCA CAAGATTCTG AAGAAAAACA AGTTCAGAGA CCGCTCAGTG 
TATGTCCCCA AGGAGGCGTA CGACAGCACG CTACCCCTCA TTAAGACAAC GCAGGCCGCT 
GCAATCATAG ATCATGAACG GATAGCTTTG GGAAATGAAG AAGGATTATT TGTTGTGCAT 
GTCACCAAAG ATGAGATTGT TAGAGTTGGT GATAATAAGA AAATCCATCA GATCGAACTC 
ATTCCAAGTG ACCAGCTTGT CGCTGTGATC TCAGGCCGAA ATCGCCACGT CCGACTTTTT 
CCTATGTCAG CCTTGGATGG GCGAGAGACG GATTTTTATA AACTGGCAGA GACTAAAGGG 
TGTCAGACCA TAGCTGCTGG GAAAGTGCGC CACGGAGCTC TGTCCTGCCT GTGTGTGGCC 
ATGAAGAGAC AGGTCCTCTG CTATGAACTG TTCCAAAGCA AGACCAGGCA CAGGAAGTTT 
AAGGAGATTC AAGTCCCATG TAATGTCCAG TGGATGGCCA TCTTCAGTGA GCATCTCTGT 
GTGGGATTCC AGTCAGGATT TCTCAGATAC CCCCTGAATG GAGAAGGAGG TCCATGCAAT 
ATGCTCCATT CAAATGACCA TACACTGTCA TTCATTTCAC ACCAACCAAT GGACGCTCTC 
TGTGCAGTTG AGATCTCCAA CAAAGAGTAC TTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACAGACTGCC AGGGCCGAAG ATCCAGACAG CAGGAACTGA TGTGGCCAGC AAATCCTTCC 
TCCTGTTGTT ACAATGCTCC ATATCTCTCC GTGTATAGTG AAAACGCAGT TGATATCTTT 
GATGTAAACT CCATGGAATG GATACAGACT CTTCCTCTCA AAAAGGTCCG ACCTTTAAAC 
ACTGAAGGAT CATTAAATCT CTTAGGATTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTTGTGGTT CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ATATCAACAA CAAACGGCGT TACTCCTTCC GAGTCCCTGA AGAAGAGAGG 
ATGCAACAGA GGAGGGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGCCAGGTTT CCCTTATTCA CCCCCTCATC ACCACTCCGG CCTGATCTCC 
TCTCCGAGCA ACTTTGAGCA CATCTATCAC ATGACTGTCA CTTCTGCTGA ACAGTTTCTC 
TCTCCTGAGT CCATAAGCCC TGGGTCCCCC CCGTCCCTGC GCTCTGCCCC TGGCACACCA 
CACTCTGTGA CTCTGAGGAA CCCACGGCCT CAGGAAAGCC GGACAGTATT CAGTGGCTCC 
GTCAGTATTC CCTCCATCAC CAAGTCCCGC CCTGAGCCAG GCCGCTCCAT GAGTGCCAGC 
AGCGGCCTGT CTGCACGGTC GTCAGCCCAG AATGGCAGTG CACTAAAGAG GGAATTCTCT 
GGAGGAAGCT ATAACACAAA GCGACAGCCC ATGCCCTCTC CCTCGGAGGG CTCTTTGTCA 
TCAGGAGGCA TGGACCAAGG AAGTGACGCC CCTGCGAGGG ACTATGATGG AGAGGACTCT 
GATTCTCCAA GGCATTCCAC AGCTTCCAAC AGCTCTAACC TGAGCAGCCC CCCAAGTCCC 
ATTTCACCCC AAAAGACCAA GAGCCTCTCC CTGGAGAGCA CAGACCGCGG GAGCTGGGAT 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496868.1
CDS	1142..6487
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006496805.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496805.3

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAAATTGCA GACTGTGCTC CACTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG CCCGGCTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAT 
CAATTTTTCG TGAAGTCTTT CACTGCCCCT ACCAAGTGTC ACCAGTGCAC CTCCTTGATG 
GTCGGTTTGA TAAGACAGGG CTGTTCCTGT GAAGTCTGTG GGTTCTCATG TCATATAACT 
TGTGTGAACA AAGCCCCGAC GGTTTGCCCA GTTCCTCCTG AGCAAACTAA AGGTCCACTG 
GGCATAGACC CCCAGAAGGG AGTTGGGACG GCGTATGAGG GTCATGTCAG GATCCCTAAG 
CCGGCGGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTG TGGTCTGCGA CTTCAAACTG 
TTTCTTTATG ATATCGCAGA GGGAAAAGCA TCGCAGCCCA CCTCCGTCAT TAGTCAAGTG 
ATTGACATGA GAGATGAAGA ATTTTCTGTG AGTTCAGTCT TAGCTTCTGA TGTTATTCAC 
GCAAGTCGAA AAGATATCCC CTGTATATTC AGGGTCACAG CTTCCCAGCT CTCAGCACCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCA GACAGCGAGA ATGAGAGGAG TAAGTGGGTG 
GGAGTGCTGA GTGAACTCCA CAAGATTCTG AAGAAAAACA AGTTCAGAGA CCGCTCAGTG 
TATGTCCCCA AGGAGGCGTA CGACAGCACG CTACCCCTCA TTAAGACAAC GCAGGCCGCT 
GCAATCATAG ATCATGAACG GATAGCTTTG GGAAATGAAG AAGGATTATT TGTTGTGCAT 
GTCACCAAAG ATGAGATTGT TAGAGTTGGT GATAATAAGA AAATCCATCA GATCGAACTC 
ATTCCAAGTG ACCAGCTTGT CGCTGTGATC TCAGGCCGAA ATCGCCACGT CCGACTTTTT 
CCTATGTCAG CCTTGGATGG GCGAGAGACG GATTTTTATA AACTGGCAGA GACTAAAGGG 
TGTCAGACCA TAGCTGCTGG GAAAGTGCGC CACGGAGCTC TGTCCTGCCT GTGTGTGGCC 
ATGAAGAGAC AGGTCCTCTG CTATGAACTG TTCCAAAGCA AGACCAGGCA CAGGAAGTTT 
AAGGAGATTC AAGTCCCATG TAATGTCCAG TGGATGGCCA TCTTCAGTGA GCATCTCTGT 
GTGGGATTCC AGTCAGGATT TCTCAGATAC CCCCTGAATG GAGAAGGAGG TCCATGCAAT 
ATGCTCCATT CAAATGACCA TACACTGTCA TTCATTTCAC ACCAACCAAT GGACGCTCTC 
TGTGCAGTTG AGATCTCCAA CAAAGAGTAC TTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACAGACTGCC AGGGCCGAAG ATCCAGACAG CAGGAACTGA TGTGGCCAGC AAATCCTTCC 
TCCTGTTGTT ACAATGCTCC ATATCTCTCC GTGTATAGTG AAAACGCAGT TGATATCTTT 
GATGTAAACT CCATGGAATG GATACAGACT CTTCCTCTCA AAAAGGTCCG ACCTTTAAAC 
ACTGAAGGAT CATTAAATCT CTTAGGATTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTTGTGGTT CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ATATCAACAA CAAACGGCGT TACTCCTTCC GAGTCCCTGA AGAAGAGAGG 
ATGCAACAGA GGAGGGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGCCAGGTTT CCCTTATTCA CCCCCTCATC ACCACTCCGG CCTGATCTCC 
TCTCCGAGCA ACTTTGAGCA CATCTATCAC ATGACTGTCA CTTCTGCTGA ACAGTTTCTC 
TCTCCTGAGT CCATAAGCCC TGGGTCCCCC CCGTCCCTGC GCTCTGCCCC TGGCACACCA 
CACTCTGTGA CTCTGAGGAA CCCACGGCCT CAGGAAAGCC GGACAGTATT CAGTGGCTCC 
GTCAGTATTC CCTCCATCAC CAAGTCCCGC CCTGAGCCAG GCCGCTCCAT GAGTGCCAGC 
AGCGGCCTGT CTGCACGGTC GTCAGCCCAG AATGGCAGTG CACTAAAGAG GGAATTCTCT 
GGAGGAAGCT ATAACACAAA GCGACAGCCC ATGCCCTCTC CCTCGGAGGG CTCTTTGTCA 
TCAGGAGGCA TGGACCAAGG AAGTGACGCC CCTGCGAGGG ACTATGATGG AGAGGACTCT 
GATTCTCCAA GGCATTCCAC AGCTTCCAAC AGCTCTAACC TGAGCAGCCC CCCAAGTCCC 
ATTTCACCCC AAAAGACCAA GAGCCTCTCC CTGGAGAGCA CAGACCGCGG GAGCTGGGAT 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_006496806.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496869.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X7, mRNA.

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CloneID	OMu28726
Clone ID Related Accession (Same CDS sequence)	XM_006496806.4 , XM_006496806.3
Accession Version	XM_006496806.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496806.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGATT CCCTTTCTTC TTCCTCATCC TCACTGATTG ATTTTTTGGA TGACATTGCA 
GACTGTGCTC CACTTCCAGC TCACACACCA ACCTTAAGGA AAAAGGGATG CCCGGCTTCA 
ACTGGCTTTC CACCTAAGCG CAAGACTCAT CAATTTTTCG TGAAGTCTTT CACTGCCCCT 
ACCAAGTGTC ACCAGTGCAC CTCCTTGATG GTCGGTTTGA TAAGACAGGG CTGTTCCTGT 
GAAGTCTGTG GGTTCTCATG TCATATAACT TGTGTGAACA AAGCCCCGAC GGTTTGCCCA 
GTTCCTCCTG AGCAAACTAA AGGTCCACTG GGCATAGACC CCCAGAAGGG AGTTGGGACG 
GCGTATGAGG GTCATGTCAG GATCCCTAAG CCGGCGGGAG TGAAGAAAGG GTGGCAGAGA 
GCACTGGCTG TGGTCTGCGA CTTCAAACTG TTTCTTTATG ATATCGCAGA GGGAAAAGCA 
TCGCAGCCCA CCTCCGTCAT TAGTCAAGTG ATTGACATGA GAGATGAAGA ATTTTCTGTG 
AGTTCAGTCT TAGCTTCTGA TGTTATTCAC GCAAGTCGAA AAGATATCCC CTGTATATTC 
AGGGTCACAG CTTCCCAGCT CTCAGCACCT AGTAACAAAT GTTCCATCCT GATGCTAGCA 
GACAGCGAGA ATGAGAGGAG TAAGTGGGTG GGAGTGCTGA GTGAACTCCA CAAGATTCTG 
AAGAAAAACA AGTTCAGAGA CCGCTCAGTG TATGTCCCCA AGGAGGCGTA CGACAGCACG 
CTACCCCTCA TTAAGACAAC GCAGGCCGCT GCAATCATAG ATCATGAACG GATAGCTTTG 
GGAAATGAAG AAGGATTATT TGTTGTGCAT GTCACCAAAG ATGAGATTGT TAGAGTTGGT 
GATAATAAGA AAATCCATCA GATCGAACTC ATTCCAAGTG ACCAGCTTGT CGCTGTGATC 
TCAGGCCGAA ATCGCCACGT CCGACTTTTT CCTATGTCAG CCTTGGATGG GCGAGAGACG 
GATTTTTATA AACTGGCAGA GACTAAAGGG TGTCAGACCA TAGCTGCTGG GAAAGTGCGC 
CACGGAGCTC TGTCCTGCCT GTGTGTGGCC ATGAAGAGAC AGGTCCTCTG CTATGAACTG 
TTCCAAAGCA AGACCAGGCA CAGGAAGTTT AAGGAGATTC AAGTCCCATG TAATGTCCAG 
TGGATGGCCA TCTTCAGTGA GCATCTCTGT GTGGGATTCC AGTCAGGATT TCTCAGATAC 
CCCCTGAATG GAGAAGGAGG TCCATGCAAT ATGCTCCATT CAAATGACCA TACACTGTCA 
TTCATTTCAC ACCAACCAAT GGACGCTCTC TGTGCAGTTG AGATCTCCAA CAAAGAGTAC 
TTGCTGTGTT TTAACAGCAT TGGGATATAC ACAGACTGCC AGGGCCGAAG ATCCAGACAG 
CAGGAACTGA TGTGGCCAGC AAATCCTTCC TCCTGTTGTT ACAATGCTCC ATATCTCTCC 
GTGTATAGTG AAAACGCAGT TGATATCTTT GATGTAAACT CCATGGAATG GATACAGACT 
CTTCCTCTCA AAAAGGTCCG ACCTTTAAAC ACTGAAGGAT CATTAAATCT CTTAGGATTG 
GAGACCATTA GATTAATATA TTTCAAAAAT AAGATGGCAG AAGGGGATGA ACTTGTGGTT 
CCTGAAACAT CAGATAATAG TCGGAAACAA ATGGTTAGAA ATATCAACAA CAAACGGCGT 
TACTCCTTCC GAGTCCCTGA AGAAGAGAGG ATGCAACAGA GGAGGGAGAT GCTACGAGAT 
CCAGAAATGA GAAATAAATT AATTTCTAAC CCAACTAATT TTAACCACAT AGCACACATG 
GGTCCTGGAG ATGGAATACA GATCCTAAAA GACCTGCCCA TGCCAGGTTT CCCTTATTCA 
CCCCCTCATC ACCACTCCGG CCTGATCTCC TCTCCGAGCA ACTTTGAGCA CATCTATCAC 
ATGACTGTCA CTTCTGCTGA ACAGTTTCTC TCTCCTGAGT CCATAAGCCC TGGGTCCCCC 
CCGTCCCTGC GCTCTGCCCC TGGCACACCA CACTCTGTGA CTCTGAGGAA CCCACGGCCT 
CAGGAAAGCC GGACAGTATT CAGTGGCTCC GTCAGTATTC CCTCCATCAC CAAGTCCCGC 
CCTGAGCCAG GCCGCTCCAT GAGTGCCAGC AGCGGCCTGT CTGCACGGTC GTCAGCCCAG 
AATGGCAGTG CACTAAAGAG GGAATTCTCT GGAGGAAGCT ATAACACAAA GCGACAGCCC 
ATGCCCTCTC CCTCGGAGGG CTCTTTGTCA TCAGGAGGCA TGGACCAAGG AAGTGACGCC 
CCTGCGAGGG ACTATGATGG AGAGGACTCT GATTCTCCAA GGCATTCCAC AGCTTCCAAC 
AGCTCTAACC TGAGCAGCCC CCCAAGTCCC ATTTCACCCC AAAAGACCAA GAGCCTCTCC 
CTGGAGAGCA CAGACCGCGG GAGCTGGGAT CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496869.1
CDS	1142..6457
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKDSLSSSSSSLIDFLDDIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006496806.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496806.3

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGATT CCCTTTCTTC TTCCTCATCC TCACTGATTG ATTTTTTGGA TGACATTGCA 
GACTGTGCTC CACTTCCAGC TCACACACCA ACCTTAAGGA AAAAGGGATG CCCGGCTTCA 
ACTGGCTTTC CACCTAAGCG CAAGACTCAT CAATTTTTCG TGAAGTCTTT CACTGCCCCT 
ACCAAGTGTC ACCAGTGCAC CTCCTTGATG GTCGGTTTGA TAAGACAGGG CTGTTCCTGT 
GAAGTCTGTG GGTTCTCATG TCATATAACT TGTGTGAACA AAGCCCCGAC GGTTTGCCCA 
GTTCCTCCTG AGCAAACTAA AGGTCCACTG GGCATAGACC CCCAGAAGGG AGTTGGGACG 
GCGTATGAGG GTCATGTCAG GATCCCTAAG CCGGCGGGAG TGAAGAAAGG GTGGCAGAGA 
GCACTGGCTG TGGTCTGCGA CTTCAAACTG TTTCTTTATG ATATCGCAGA GGGAAAAGCA 
TCGCAGCCCA CCTCCGTCAT TAGTCAAGTG ATTGACATGA GAGATGAAGA ATTTTCTGTG 
AGTTCAGTCT TAGCTTCTGA TGTTATTCAC GCAAGTCGAA AAGATATCCC CTGTATATTC 
AGGGTCACAG CTTCCCAGCT CTCAGCACCT AGTAACAAAT GTTCCATCCT GATGCTAGCA 
GACAGCGAGA ATGAGAGGAG TAAGTGGGTG GGAGTGCTGA GTGAACTCCA CAAGATTCTG 
AAGAAAAACA AGTTCAGAGA CCGCTCAGTG TATGTCCCCA AGGAGGCGTA CGACAGCACG 
CTACCCCTCA TTAAGACAAC GCAGGCCGCT GCAATCATAG ATCATGAACG GATAGCTTTG 
GGAAATGAAG AAGGATTATT TGTTGTGCAT GTCACCAAAG ATGAGATTGT TAGAGTTGGT 
GATAATAAGA AAATCCATCA GATCGAACTC ATTCCAAGTG ACCAGCTTGT CGCTGTGATC 
TCAGGCCGAA ATCGCCACGT CCGACTTTTT CCTATGTCAG CCTTGGATGG GCGAGAGACG 
GATTTTTATA AACTGGCAGA GACTAAAGGG TGTCAGACCA TAGCTGCTGG GAAAGTGCGC 
CACGGAGCTC TGTCCTGCCT GTGTGTGGCC ATGAAGAGAC AGGTCCTCTG CTATGAACTG 
TTCCAAAGCA AGACCAGGCA CAGGAAGTTT AAGGAGATTC AAGTCCCATG TAATGTCCAG 
TGGATGGCCA TCTTCAGTGA GCATCTCTGT GTGGGATTCC AGTCAGGATT TCTCAGATAC 
CCCCTGAATG GAGAAGGAGG TCCATGCAAT ATGCTCCATT CAAATGACCA TACACTGTCA 
TTCATTTCAC ACCAACCAAT GGACGCTCTC TGTGCAGTTG AGATCTCCAA CAAAGAGTAC 
TTGCTGTGTT TTAACAGCAT TGGGATATAC ACAGACTGCC AGGGCCGAAG ATCCAGACAG 
CAGGAACTGA TGTGGCCAGC AAATCCTTCC TCCTGTTGTT ACAATGCTCC ATATCTCTCC 
GTGTATAGTG AAAACGCAGT TGATATCTTT GATGTAAACT CCATGGAATG GATACAGACT 
CTTCCTCTCA AAAAGGTCCG ACCTTTAAAC ACTGAAGGAT CATTAAATCT CTTAGGATTG 
GAGACCATTA GATTAATATA TTTCAAAAAT AAGATGGCAG AAGGGGATGA ACTTGTGGTT 
CCTGAAACAT CAGATAATAG TCGGAAACAA ATGGTTAGAA ATATCAACAA CAAACGGCGT 
TACTCCTTCC GAGTCCCTGA AGAAGAGAGG ATGCAACAGA GGAGGGAGAT GCTACGAGAT 
CCAGAAATGA GAAATAAATT AATTTCTAAC CCAACTAATT TTAACCACAT AGCACACATG 
GGTCCTGGAG ATGGAATACA GATCCTAAAA GACCTGCCCA TGCCAGGTTT CCCTTATTCA 
CCCCCTCATC ACCACTCCGG CCTGATCTCC TCTCCGAGCA ACTTTGAGCA CATCTATCAC 
ATGACTGTCA CTTCTGCTGA ACAGTTTCTC TCTCCTGAGT CCATAAGCCC TGGGTCCCCC 
CCGTCCCTGC GCTCTGCCCC TGGCACACCA CACTCTGTGA CTCTGAGGAA CCCACGGCCT 
CAGGAAAGCC GGACAGTATT CAGTGGCTCC GTCAGTATTC CCTCCATCAC CAAGTCCCGC 
CCTGAGCCAG GCCGCTCCAT GAGTGCCAGC AGCGGCCTGT CTGCACGGTC GTCAGCCCAG 
AATGGCAGTG CACTAAAGAG GGAATTCTCT GGAGGAAGCT ATAACACAAA GCGACAGCCC 
ATGCCCTCTC CCTCGGAGGG CTCTTTGTCA TCAGGAGGCA TGGACCAAGG AAGTGACGCC 
CCTGCGAGGG ACTATGATGG AGAGGACTCT GATTCTCCAA GGCATTCCAC AGCTTCCAAC 
AGCTCTAACC TGAGCAGCCC CCCAAGTCCC ATTTCACCCC AAAAGACCAA GAGCCTCTCC 
CTGGAGAGCA CAGACCGCGG GAGCTGGGAT CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_006496807.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496870.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X8, mRNA.

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CloneID	OMu28727
Clone ID Related Accession (Same CDS sequence)	XM_006496807.3 , XM_006496807.4
Accession Version	XM_006496807.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496807.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGCTA GCAAAGGCAG ACGTATTGCA GACTGTGCTC CACTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG CCCGGCTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAT 
CAATTTTTCG TGAAGTCTTT CACTGCCCCT ACCAAGTGTC ACCAGTGCAC CTCCTTGATG 
GTCGGTTTGA TAAGACAGGG CTGTTCCTGT GAAGTCTGTG GGTTCTCATG TCATATAACT 
TGTGTGAACA AAGCCCCGAC GGTTTGCCCA GTTCCTCCTG AGCAAACTAA AGGTCCACTG 
GGCATAGACC CCCAGAAGGG AGTTGGGACG GCGTATGAGG GTCATGTCAG GATCCCTAAG 
CCGGCGGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTG TGGTCTGCGA CTTCAAACTG 
TTTCTTTATG ATATCGCAGA GGGAAAAGCA TCGCAGCCCA CCTCCGTCAT TAGTCAAGTG 
ATTGACATGA GAGATGAAGA ATTTTCTGTG AGTTCAGTCT TAGCTTCTGA TGTTATTCAC 
GCAAGTCGAA AAGATATCCC CTGTATATTC AGGGTCACAG CTTCCCAGCT CTCAGCACCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCA GACAGCGAGA ATGAGAGGAG TAAGTGGGTG 
GGAGTGCTGA GTGAACTCCA CAAGATTCTG AAGAAAAACA AGTTCAGAGA CCGCTCAGTG 
TATGTCCCCA AGGAGGCGTA CGACAGCACG CTACCCCTCA TTAAGACAAC GCAGGCCGCT 
GCAATCATAG ATCATGAACG GATAGCTTTG GGAAATGAAG AAGGATTATT TGTTGTGCAT 
GTCACCAAAG ATGAGATTGT TAGAGTTGGT GATAATAAGA AAATCCATCA GATCGAACTC 
ATTCCAAGTG ACCAGCTTGT CGCTGTGATC TCAGGCCGAA ATCGCCACGT CCGACTTTTT 
CCTATGTCAG CCTTGGATGG GCGAGAGACG GATTTTTATA AACTGGCAGA GACTAAAGGG 
TGTCAGACCA TAGCTGCTGG GAAAGTGCGC CACGGAGCTC TGTCCTGCCT GTGTGTGGCC 
ATGAAGAGAC AGGTCCTCTG CTATGAACTG TTCCAAAGCA AGACCAGGCA CAGGAAGTTT 
AAGGAGATTC AAGTCCCATG TAATGTCCAG TGGATGGCCA TCTTCAGTGA GCATCTCTGT 
GTGGGATTCC AGTCAGGATT TCTCAGATAC CCCCTGAATG GAGAAGGAGG TCCATGCAAT 
ATGCTCCATT CAAATGACCA TACACTGTCA TTCATTTCAC ACCAACCAAT GGACGCTCTC 
TGTGCAGTTG AGATCTCCAA CAAAGAGTAC TTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACAGACTGCC AGGGCCGAAG ATCCAGACAG CAGGAACTGA TGTGGCCAGC AAATCCTTCC 
TCCTGTTGTT ACAATGCTCC ATATCTCTCC GTGTATAGTG AAAACGCAGT TGATATCTTT 
GATGTAAACT CCATGGAATG GATACAGACT CTTCCTCTCA AAAAGGTCCG ACCTTTAAAC 
ACTGAAGGAT CATTAAATCT CTTAGGATTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTTGTGGTT CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ATATCAACAA CAAACGGCGT TACTCCTTCC GAGTCCCTGA AGAAGAGAGG 
ATGCAACAGA GGAGGGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGCCAGGTTT CCCTTATTCA CCCCCTCATC ACCACTCCGG CCTGATCTCC 
TCTCCGAGCA ACTTTGAGCA CATCTATCAC ATGACTGTCA CTTCTGCTGA ACAGTTTCTC 
TCTCCTGAGT CCATAAGCCC TGGGTCCCCC CCGTCCCTGC GCTCTGCCCC TGGCACACCA 
CACTCTGTGA CTCTGAGGAA CCCACGGCCT CAGGAAAGCC GGACAGTATT CAGTGGCTCC 
GTCAGTATTC CCTCCATCAC CAAGTCCCGC CCTGAGCCAG GCCGCTCCAT GAGTGCCAGC 
AGCGGCCTGT CTGCACGGTC GTCAGCCCAG AATGGCAGTG CACTAAAGAG GGAATTCTCT 
GGAGGAAGCT ATAACACAAA GCGACAGCCC ATGCCCTCTC CCTCGGAGGG CTCTTTGTCA 
TCAGGAGGCA TGGACCAAGG AAGTGACGCC CCTGCGAGGG ACTATGATGG AGAGGACTCT 
GATTCTCCAA GGCATTCCAC AGCTTCCAAC AGCTCTAACC TGAGCAGCCC CCCAAGTCCC 
ATTTCACCCC AAAAGACCAA GAGCCTCTCC CTGGAGAGCA CAGACCGCGG GAGCTGGGAT 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496870.1
CDS	1142..6427
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006496807.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496807.3

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGCTA GCAAAGGCAG ACGTATTGCA GACTGTGCTC CACTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG CCCGGCTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAT 
CAATTTTTCG TGAAGTCTTT CACTGCCCCT ACCAAGTGTC ACCAGTGCAC CTCCTTGATG 
GTCGGTTTGA TAAGACAGGG CTGTTCCTGT GAAGTCTGTG GGTTCTCATG TCATATAACT 
TGTGTGAACA AAGCCCCGAC GGTTTGCCCA GTTCCTCCTG AGCAAACTAA AGGTCCACTG 
GGCATAGACC CCCAGAAGGG AGTTGGGACG GCGTATGAGG GTCATGTCAG GATCCCTAAG 
CCGGCGGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTG TGGTCTGCGA CTTCAAACTG 
TTTCTTTATG ATATCGCAGA GGGAAAAGCA TCGCAGCCCA CCTCCGTCAT TAGTCAAGTG 
ATTGACATGA GAGATGAAGA ATTTTCTGTG AGTTCAGTCT TAGCTTCTGA TGTTATTCAC 
GCAAGTCGAA AAGATATCCC CTGTATATTC AGGGTCACAG CTTCCCAGCT CTCAGCACCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCA GACAGCGAGA ATGAGAGGAG TAAGTGGGTG 
GGAGTGCTGA GTGAACTCCA CAAGATTCTG AAGAAAAACA AGTTCAGAGA CCGCTCAGTG 
TATGTCCCCA AGGAGGCGTA CGACAGCACG CTACCCCTCA TTAAGACAAC GCAGGCCGCT 
GCAATCATAG ATCATGAACG GATAGCTTTG GGAAATGAAG AAGGATTATT TGTTGTGCAT 
GTCACCAAAG ATGAGATTGT TAGAGTTGGT GATAATAAGA AAATCCATCA GATCGAACTC 
ATTCCAAGTG ACCAGCTTGT CGCTGTGATC TCAGGCCGAA ATCGCCACGT CCGACTTTTT 
CCTATGTCAG CCTTGGATGG GCGAGAGACG GATTTTTATA AACTGGCAGA GACTAAAGGG 
TGTCAGACCA TAGCTGCTGG GAAAGTGCGC CACGGAGCTC TGTCCTGCCT GTGTGTGGCC 
ATGAAGAGAC AGGTCCTCTG CTATGAACTG TTCCAAAGCA AGACCAGGCA CAGGAAGTTT 
AAGGAGATTC AAGTCCCATG TAATGTCCAG TGGATGGCCA TCTTCAGTGA GCATCTCTGT 
GTGGGATTCC AGTCAGGATT TCTCAGATAC CCCCTGAATG GAGAAGGAGG TCCATGCAAT 
ATGCTCCATT CAAATGACCA TACACTGTCA TTCATTTCAC ACCAACCAAT GGACGCTCTC 
TGTGCAGTTG AGATCTCCAA CAAAGAGTAC TTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACAGACTGCC AGGGCCGAAG ATCCAGACAG CAGGAACTGA TGTGGCCAGC AAATCCTTCC 
TCCTGTTGTT ACAATGCTCC ATATCTCTCC GTGTATAGTG AAAACGCAGT TGATATCTTT 
GATGTAAACT CCATGGAATG GATACAGACT CTTCCTCTCA AAAAGGTCCG ACCTTTAAAC 
ACTGAAGGAT CATTAAATCT CTTAGGATTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTTGTGGTT CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ATATCAACAA CAAACGGCGT TACTCCTTCC GAGTCCCTGA AGAAGAGAGG 
ATGCAACAGA GGAGGGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGCCAGGTTT CCCTTATTCA CCCCCTCATC ACCACTCCGG CCTGATCTCC 
TCTCCGAGCA ACTTTGAGCA CATCTATCAC ATGACTGTCA CTTCTGCTGA ACAGTTTCTC 
TCTCCTGAGT CCATAAGCCC TGGGTCCCCC CCGTCCCTGC GCTCTGCCCC TGGCACACCA 
CACTCTGTGA CTCTGAGGAA CCCACGGCCT CAGGAAAGCC GGACAGTATT CAGTGGCTCC 
GTCAGTATTC CCTCCATCAC CAAGTCCCGC CCTGAGCCAG GCCGCTCCAT GAGTGCCAGC 
AGCGGCCTGT CTGCACGGTC GTCAGCCCAG AATGGCAGTG CACTAAAGAG GGAATTCTCT 
GGAGGAAGCT ATAACACAAA GCGACAGCCC ATGCCCTCTC CCTCGGAGGG CTCTTTGTCA 
TCAGGAGGCA TGGACCAAGG AAGTGACGCC CCTGCGAGGG ACTATGATGG AGAGGACTCT 
GATTCTCCAA GGCATTCCAC AGCTTCCAAC AGCTCTAACC TGAGCAGCCC CCCAAGTCCC 
ATTTCACCCC AAAAGACCAA GAGCCTCTCC CTGGAGAGCA CAGACCGCGG GAGCTGGGAT 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_006496808.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496871.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X9, mRNA.

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CloneID	OMu28728
Clone ID Related Accession (Same CDS sequence)	XM_006496808.3 , NM_001359542.1
Accession Version	XM_006496808.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496808.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAAGATTCC CTTTCTTCTT CCTCATCCTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC GTCCTGTACT CCAGCTAGCA AAGGCAGACG TATTGCAGAC 
TGTGCTCCAC TTCCAGCTCA CACACCAACC TTAAGGAAAA AGGGATGCCC GGCTTCAACT 
GGCTTTCCAC CTAAGCGCAA GACTCATCAA TTTTTCGTGA AGTCTTTCAC TGCCCCTACC 
AAGTGTCACC AGTGCACCTC CTTGATGGTC GGTTTGATAA GACAGGGCTG TTCCTGTGAA 
GTCTGTGGGT TCTCATGTCA TATAACTTGT GTGAACAAAG CCCCGACGGT TTGCCCAGTT 
CCTCCTGAGC AAACTAAAGG TCCACTGGGC ATAGACCCCC AGAAGGGAGT TGGGACGGCG 
TATGAGGGTC ATGTCAGGAT CCCTAAGCCG GCGGGAGTGA AGAAAGGGTG GCAGAGAGCA 
CTGGCTGTGG TCTGCGACTT CAAACTGTTT CTTTATGATA TCGCAGAGGG AAAAGCATCG 
CAGCCCACCT CCGTCATTAG TCAAGTGATT GACATGAGAG ATGAAGAATT TTCTGTGAGT 
TCAGTCTTAG CTTCTGATGT TATTCACGCA AGTCGAAAAG ATATCCCCTG TATATTCAGG 
GTCACAGCTT CCCAGCTCTC AGCACCTAGT AACAAATGTT CCATCCTGAT GCTAGCAGAC 
AGCGAGAATG AGAGGAGTAA GTGGGTGGGA GTGCTGAGTG AACTCCACAA GATTCTGAAG 
AAAAACAAGT TCAGAGACCG CTCAGTGTAT GTCCCCAAGG AGGCGTACGA CAGCACGCTA 
CCCCTCATTA AGACAACGCA GGCCGCTGCA ATCATAGATC ATGAACGGAT AGCTTTGGGA 
AATGAAGAAG GATTATTTGT TGTGCATGTC ACCAAAGATG AGATTGTTAG AGTTGGTGAT 
AATAAGAAAA TCCATCAGAT CGAACTCATT CCAAGTGACC AGCTTGTCGC TGTGATCTCA 
GGCCGAAATC GCCACGTCCG ACTTTTTCCT ATGTCAGCCT TGGATGGGCG AGAGACGGAT 
TTTTATAAAC TGGCAGAGAC TAAAGGGTGT CAGACCATAG CTGCTGGGAA AGTGCGCCAC 
GGAGCTCTGT CCTGCCTGTG TGTGGCCATG AAGAGACAGG TCCTCTGCTA TGAACTGTTC 
CAAAGCAAGA CCAGGCACAG GAAGTTTAAG GAGATTCAAG TCCCATGTAA TGTCCAGTGG 
ATGGCCATCT TCAGTGAGCA TCTCTGTGTG GGATTCCAGT CAGGATTTCT CAGATACCCC 
CTGAATGGAG AAGGAGGTCC ATGCAATATG CTCCATTCAA ATGACCATAC ACTGTCATTC 
ATTTCACACC AACCAATGGA CGCTCTCTGT GCAGTTGAGA TCTCCAACAA AGAGTACTTG 
CTGTGTTTTA ACAGCATTGG GATATACACA GACTGCCAGG GCCGAAGATC CAGACAGCAG 
GAACTGATGT GGCCAGCAAA TCCTTCCTCC TGTTGTTACA ATGCTCCATA TCTCTCCGTG 
TATAGTGAAA ACGCAGTTGA TATCTTTGAT GTAAACTCCA TGGAATGGAT ACAGACTCTT 
CCTCTCAAAA AGGTCCGACC TTTAAACACT GAAGGATCAT TAAATCTCTT AGGATTGGAG 
ACCATTAGAT TAATATATTT CAAAAATAAG ATGGCAGAAG GGGATGAACT TGTGGTTCCT 
GAAACATCAG ATAATAGTCG GAAACAAATG GTTAGAAATA TCAACAACAA ACGGCGTTAC 
TCCTTCCGAG TCCCTGAAGA AGAGAGGATG CAACAGAGGA GGGAGATGCT ACGAGATCCA 
GAAATGAGAA ATAAATTAAT TTCTAACCCA ACTAATTTTA ACCACATAGC ACACATGGGT 
CCTGGAGATG GAATACAGAT CCTAAAAGAC CTGCCCATGA ACCCACGGCC TCAGGAAAGC 
CGGACAGTAT TCAGTGGCTC CGTCAGTATT CCCTCCATCA CCAAGTCCCG CCCTGAGCCA 
GGCCGCTCCA TGAGTGCCAG CAGCGGCCTG TCTGCACGGT CGTCAGCCCA GAATGGCAGT 
GCACTAAAGA GGGAATTCTC TGGAGGAAGC TATAACACAA AGCGACAGCC CATGCCCTCT 
CCCTCGGAGG GCTCTTTGTC ATCAGGAGGC ATGGACCAAG GAAGTGACGC CCCTGCGAGG 
GACTATGATG GAGAGGACTC TGATTCTCCA AGGCATTCCA CAGCTTCCAA CAGCTCTAAC 
CTGAGCAGCC CCCCAAGTCC CATTTCACCC CAAAAGACCA AGAGCCTCTC CCTGGAGAGC 
ACAGACCGCG GGAGCTGGGA TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496871.1
CDS	1142..6388
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSLSSSSSSLIDFLDDRSPSCTPASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006496808.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496808.3

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGACCAATT TGAAGATTCC CTTTCTTCTT CCTCATCCTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC GTCCTGTACT CCAGCTAGCA AAGGCAGACG TATTGCAGAC 
TGTGCTCCAC TTCCAGCTCA CACACCAACC TTAAGGAAAA AGGGATGCCC GGCTTCAACT 
GGCTTTCCAC CTAAGCGCAA GACTCATCAA TTTTTCGTGA AGTCTTTCAC TGCCCCTACC 
AAGTGTCACC AGTGCACCTC CTTGATGGTC GGTTTGATAA GACAGGGCTG TTCCTGTGAA 
GTCTGTGGGT TCTCATGTCA TATAACTTGT GTGAACAAAG CCCCGACGGT TTGCCCAGTT 
CCTCCTGAGC AAACTAAAGG TCCACTGGGC ATAGACCCCC AGAAGGGAGT TGGGACGGCG 
TATGAGGGTC ATGTCAGGAT CCCTAAGCCG GCGGGAGTGA AGAAAGGGTG GCAGAGAGCA 
CTGGCTGTGG TCTGCGACTT CAAACTGTTT CTTTATGATA TCGCAGAGGG AAAAGCATCG 
CAGCCCACCT CCGTCATTAG TCAAGTGATT GACATGAGAG ATGAAGAATT TTCTGTGAGT 
TCAGTCTTAG CTTCTGATGT TATTCACGCA AGTCGAAAAG ATATCCCCTG TATATTCAGG 
GTCACAGCTT CCCAGCTCTC AGCACCTAGT AACAAATGTT CCATCCTGAT GCTAGCAGAC 
AGCGAGAATG AGAGGAGTAA GTGGGTGGGA GTGCTGAGTG AACTCCACAA GATTCTGAAG 
AAAAACAAGT TCAGAGACCG CTCAGTGTAT GTCCCCAAGG AGGCGTACGA CAGCACGCTA 
CCCCTCATTA AGACAACGCA GGCCGCTGCA ATCATAGATC ATGAACGGAT AGCTTTGGGA 
AATGAAGAAG GATTATTTGT TGTGCATGTC ACCAAAGATG AGATTGTTAG AGTTGGTGAT 
AATAAGAAAA TCCATCAGAT CGAACTCATT CCAAGTGACC AGCTTGTCGC TGTGATCTCA 
GGCCGAAATC GCCACGTCCG ACTTTTTCCT ATGTCAGCCT TGGATGGGCG AGAGACGGAT 
TTTTATAAAC TGGCAGAGAC TAAAGGGTGT CAGACCATAG CTGCTGGGAA AGTGCGCCAC 
GGAGCTCTGT CCTGCCTGTG TGTGGCCATG AAGAGACAGG TCCTCTGCTA TGAACTGTTC 
CAAAGCAAGA CCAGGCACAG GAAGTTTAAG GAGATTCAAG TCCCATGTAA TGTCCAGTGG 
ATGGCCATCT TCAGTGAGCA TCTCTGTGTG GGATTCCAGT CAGGATTTCT CAGATACCCC 
CTGAATGGAG AAGGAGGTCC ATGCAATATG CTCCATTCAA ATGACCATAC ACTGTCATTC 
ATTTCACACC AACCAATGGA CGCTCTCTGT GCAGTTGAGA TCTCCAACAA AGAGTACTTG 
CTGTGTTTTA ACAGCATTGG GATATACACA GACTGCCAGG GCCGAAGATC CAGACAGCAG 
GAACTGATGT GGCCAGCAAA TCCTTCCTCC TGTTGTTACA ATGCTCCATA TCTCTCCGTG 
TATAGTGAAA ACGCAGTTGA TATCTTTGAT GTAAACTCCA TGGAATGGAT ACAGACTCTT 
CCTCTCAAAA AGGTCCGACC TTTAAACACT GAAGGATCAT TAAATCTCTT AGGATTGGAG 
ACCATTAGAT TAATATATTT CAAAAATAAG ATGGCAGAAG GGGATGAACT TGTGGTTCCT 
GAAACATCAG ATAATAGTCG GAAACAAATG GTTAGAAATA TCAACAACAA ACGGCGTTAC 
TCCTTCCGAG TCCCTGAAGA AGAGAGGATG CAACAGAGGA GGGAGATGCT ACGAGATCCA 
GAAATGAGAA ATAAATTAAT TTCTAACCCA ACTAATTTTA ACCACATAGC ACACATGGGT 
CCTGGAGATG GAATACAGAT CCTAAAAGAC CTGCCCATGA ACCCACGGCC TCAGGAAAGC 
CGGACAGTAT TCAGTGGCTC CGTCAGTATT CCCTCCATCA CCAAGTCCCG CCCTGAGCCA 
GGCCGCTCCA TGAGTGCCAG CAGCGGCCTG TCTGCACGGT CGTCAGCCCA GAATGGCAGT 
GCACTAAAGA GGGAATTCTC TGGAGGAAGC TATAACACAA AGCGACAGCC CATGCCCTCT 
CCCTCGGAGG GCTCTTTGTC ATCAGGAGGC ATGGACCAAG GAAGTGACGC CCCTGCGAGG 
GACTATGATG GAGAGGACTC TGATTCTCCA AGGCATTCCA CAGCTTCCAA CAGCTCTAAC 
CTGAGCAGCC CCCCAAGTCC CATTTCACCC CAAAAGACCA AGAGCCTCTC CCTGGAGAGC 
ACAGACCGCG GGAGCTGGGA TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_006496809.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496872.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X12, mRNA.

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CloneID	OMu28729
Clone ID Related Accession (Same CDS sequence)	XM_006496809.3 , XM_006496809.4
Accession Version	XM_006496809.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496809.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGCTG GAAGTTCACA CCGAAGCTCT AATTGCTGAA 
GCATCAAAGG ACAAGAAGCT CCGAGAGCAG AGCGAACATT ATTCTAAGCA ACTGGAAAAC 
GAGTTGGAGG GCCTGAAGCA AAAACAAATT AGTTACTCAC CAGGAATATG CAGCATAGAA 
CATCAGCAAG AGATAACTAA ACTGAAAACT GACTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAAATTT CTAAAAGAGA AGGAATTCAC GCAAGTGAGA TTAAAAACCT GAAGAAGGAG 
CTGCATGACT CAGAAGGCCA GCAGCTTGCT CTCAACAAAG AGATTCTGGT TTTAAAAGAC 
AAATTGGAAA AAACCAGGAG AGAAAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAACAACAAT ATGAACGGGA AAAAGTATTA TTAACTGAAG AGAATAAAAA GTTAACGAGT 
GAACTCGATA AGCTGACTTC ACTGTATGAG AGCTTAAGTT TGCGCAACCA GCACTTAGAA 
GAAGAAGTCA AAGATCTAGC AGATAAGAAG GAATCAGTTG CCCACTGGGA AGCCCAAATC 
ACAGAGATAA TTCAGTGGGT GAGCGATGAG AAGGATGCAC GAGGCTATCT TCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA GTTGGAAGCA TTAAGAAATT CCAGCTTGGG GACACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCAAAACTGG ACATGTCGGC TAGACTAGAG 
TTGCAGTCTG CTCTGGATGC AGAAATAAGA GCTAAGCAGG CCATCCAGGA AGAGCTGAAT 
AAAGTCAAAG CATCTAACAT CTTAACAGAA TGTAAACTGA AAGATTCAGA GAAGAAGAAC 
TTGGAACTGT TGTCCGAAAT CGAGCAGCTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ACCAATTTGA AGATTCCCTT TCTTCTTCCT CATCCTCACT GATTGATTTT 
TTGGATGACC GCTCTCCGTC CTGTACTCCA GCTAGCAAAG GCAGACGTAT TGCAGACTGT 
GCTCCACTTC CAGCTCACAC ACCAACCTTA AGGAAAAAGG GATGCCCGGC TTCAACTGGC 
TTTCCACCTA AGCGCAAGAC TCATCAATTT TTCGTGAAGT CTTTCACTGC CCCTACCAAG 
TGTCACCAGT GCACCTCCTT GATGGTCGGT TTGATAAGAC AGGGCTGTTC CTGTGAAGTC 
TGTGGGTTCT CATGTCATAT AACTTGTGTG AACAAAGCCC CGACGGTTTG CCCAGTTCCT 
CCTGAGCAAA CTAAAGGTCC ACTGGGCATA GACCCCCAGA AGGGAGTTGG GACGGCGTAT 
GAGGGTCATG TCAGGATCCC TAAGCCGGCG GGAGTGAAGA AAGGGTGGCA GAGAGCACTG 
GCTGTGGTCT GCGACTTCAA ACTGTTTCTT TATGATATCG CAGAGGGAAA AGCATCGCAG 
CCCACCTCCG TCATTAGTCA AGTGATTGAC ATGAGAGATG AAGAATTTTC TGTGAGTTCA 
GTCTTAGCTT CTGATGTTAT TCACGCAAGT CGAAAAGATA TCCCCTGTAT ATTCAGGGTC 
ACAGCTTCCC AGCTCTCAGC ACCTAGTAAC AAATGTTCCA TCCTGATGCT AGCAGACAGC 
GAGAATGAGA GGAGTAAGTG GGTGGGAGTG CTGAGTGAAC TCCACAAGAT TCTGAAGAAA 
AACAAGTTCA GAGACCGCTC AGTGTATGTC CCCAAGGAGG CGTACGACAG CACGCTACCC 
CTCATTAAGA CAACGCAGGC CGCTGCAATC ATAGATCATG AACGGATAGC TTTGGGAAAT 
GAAGAAGGAT TATTTGTTGT GCATGTCACC AAAGATGAGA TTGTTAGAGT TGGTGATAAT 
AAGAAAATCC ATCAGATCGA ACTCATTCCA AGTGACCAGC TTGTCGCTGT GATCTCAGGC 
CGAAATCGCC ACGTCCGACT TTTTCCTATG TCAGCCTTGG ATGGGCGAGA GACGGATTTT 
TATAAACTGG CAGAGACTAA AGGGTGTCAG ACCATAGCTG CTGGGAAAGT GCGCCACGGA 
GCTCTGTCCT GCCTGTGTGT GGCCATGAAG AGACAGGTCC TCTGCTATGA ACTGTTCCAA 
AGCAAGACCA GGCACAGGAA GTTTAAGGAG ATTCAAGTCC CATGTAATGT CCAGTGGATG 
GCCATCTTCA GTGAGCATCT CTGTGTGGGA TTCCAGTCAG GATTTCTCAG ATACCCCCTG 
AATGGAGAAG GAGGTCCATG CAATATGCTC CATTCAAATG ACCATACACT GTCATTCATT 
TCACACCAAC CAATGGACGC TCTCTGTGCA GTTGAGATCT CCAACAAAGA GTACTTGCTG 
TGTTTTAACA GCATTGGGAT ATACACAGAC TGCCAGGGCC GAAGATCCAG ACAGCAGGAA 
CTGATGTGGC CAGCAAATCC TTCCTCCTGT TGTTACAATG CTCCATATCT CTCCGTGTAT 
AGTGAAAACG CAGTTGATAT CTTTGATGTA AACTCCATGG AATGGATACA GACTCTTCCT 
CTCAAAAAGG TCCGACCTTT AAACACTGAA GGATCATTAA ATCTCTTAGG ATTGGAGACC 
ATTAGATTAA TATATTTCAA AAATAAGATG GCAGAAGGGG ATGAACTTGT GGTTCCTGAA 
ACATCAGATA ATAGTCGGAA ACAAATGGTT AGAAATATCA ACAACAAACG GCGTTACTCC 
TTCCGAGTCC CTGAAGAAGA GAGGATGCAA CAGAGGAGGG AGATGCTACG AGATCCAGAA 
ATGAGAAATA AATTAATTTC TAACCCAACT AATTTTAACC ACATAGCACA CATGGGTCCT 
GGAGATGGAA TACAGATCCT AAAAGACCTG CCCATGCCAG GTTTCCCTTA TTCACCCCCT 
CATCACCACT CCGGCCTGAT CTCCTCTCCG AGCAACTTTG AGCACATCTA TCACATGACT 
GTCACTTCTG CTGAACAGTT TCTCTCTCCT GAGTCCATAA GCCCTGGGTC CCCCCCGTCC 
CTGCGCTCTG CCCCTGGCAC ACCACACTCT GTGACTCTGA GGAACCCACG GCCTCAGGAA 
AGCCGGACAG TATTCAGTGG CTCCGTCAGT ATTCCCTCCA TCACCAAGTC CCGCCCTGAG 
CCAGGCCGCT CCATGAGTGC CAGCAGCGGC CTGTCTGCAC GGTCGTCAGC CCAGAATGGC 
AGTGCACTAA AGAGGGAATT CTCTGGAGGA AGCTATAACA CAAAGCGACA GCCCATGCCC 
TCTCCCTCGG AGGGCTCTTT GTCATCAGGA GGCATGGACC AAGGAAGTGA CGCCCCTGCG 
AGGGACTATG ATGGAGAGGA CTCTGATTCT CCAAGGCATT CCACAGCTTC CAACAGCTCT 
AACCTGAGCA GCCCCCCAAG TCCCATTTCA CCCCAAAAGA CCAAGAGCCT CTCCCTGGAG 
AGCACAGACC GCGGGAGCTG GGATCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496872.1
CDS	1142..6331
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKLEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSLSSSSSSLIDFLDDRSPSCTPASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006496809.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496809.3

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGCTG GAAGTTCACA CCGAAGCTCT AATTGCTGAA 
GCATCAAAGG ACAAGAAGCT CCGAGAGCAG AGCGAACATT ATTCTAAGCA ACTGGAAAAC 
GAGTTGGAGG GCCTGAAGCA AAAACAAATT AGTTACTCAC CAGGAATATG CAGCATAGAA 
CATCAGCAAG AGATAACTAA ACTGAAAACT GACTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAAATTT CTAAAAGAGA AGGAATTCAC GCAAGTGAGA TTAAAAACCT GAAGAAGGAG 
CTGCATGACT CAGAAGGCCA GCAGCTTGCT CTCAACAAAG AGATTCTGGT TTTAAAAGAC 
AAATTGGAAA AAACCAGGAG AGAAAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAACAACAAT ATGAACGGGA AAAAGTATTA TTAACTGAAG AGAATAAAAA GTTAACGAGT 
GAACTCGATA AGCTGACTTC ACTGTATGAG AGCTTAAGTT TGCGCAACCA GCACTTAGAA 
GAAGAAGTCA AAGATCTAGC AGATAAGAAG GAATCAGTTG CCCACTGGGA AGCCCAAATC 
ACAGAGATAA TTCAGTGGGT GAGCGATGAG AAGGATGCAC GAGGCTATCT TCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA GTTGGAAGCA TTAAGAAATT CCAGCTTGGG GACACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCAAAACTGG ACATGTCGGC TAGACTAGAG 
TTGCAGTCTG CTCTGGATGC AGAAATAAGA GCTAAGCAGG CCATCCAGGA AGAGCTGAAT 
AAAGTCAAAG CATCTAACAT CTTAACAGAA TGTAAACTGA AAGATTCAGA GAAGAAGAAC 
TTGGAACTGT TGTCCGAAAT CGAGCAGCTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ACCAATTTGA AGATTCCCTT TCTTCTTCCT CATCCTCACT GATTGATTTT 
TTGGATGACC GCTCTCCGTC CTGTACTCCA GCTAGCAAAG GCAGACGTAT TGCAGACTGT 
GCTCCACTTC CAGCTCACAC ACCAACCTTA AGGAAAAAGG GATGCCCGGC TTCAACTGGC 
TTTCCACCTA AGCGCAAGAC TCATCAATTT TTCGTGAAGT CTTTCACTGC CCCTACCAAG 
TGTCACCAGT GCACCTCCTT GATGGTCGGT TTGATAAGAC AGGGCTGTTC CTGTGAAGTC 
TGTGGGTTCT CATGTCATAT AACTTGTGTG AACAAAGCCC CGACGGTTTG CCCAGTTCCT 
CCTGAGCAAA CTAAAGGTCC ACTGGGCATA GACCCCCAGA AGGGAGTTGG GACGGCGTAT 
GAGGGTCATG TCAGGATCCC TAAGCCGGCG GGAGTGAAGA AAGGGTGGCA GAGAGCACTG 
GCTGTGGTCT GCGACTTCAA ACTGTTTCTT TATGATATCG CAGAGGGAAA AGCATCGCAG 
CCCACCTCCG TCATTAGTCA AGTGATTGAC ATGAGAGATG AAGAATTTTC TGTGAGTTCA 
GTCTTAGCTT CTGATGTTAT TCACGCAAGT CGAAAAGATA TCCCCTGTAT ATTCAGGGTC 
ACAGCTTCCC AGCTCTCAGC ACCTAGTAAC AAATGTTCCA TCCTGATGCT AGCAGACAGC 
GAGAATGAGA GGAGTAAGTG GGTGGGAGTG CTGAGTGAAC TCCACAAGAT TCTGAAGAAA 
AACAAGTTCA GAGACCGCTC AGTGTATGTC CCCAAGGAGG CGTACGACAG CACGCTACCC 
CTCATTAAGA CAACGCAGGC CGCTGCAATC ATAGATCATG AACGGATAGC TTTGGGAAAT 
GAAGAAGGAT TATTTGTTGT GCATGTCACC AAAGATGAGA TTGTTAGAGT TGGTGATAAT 
AAGAAAATCC ATCAGATCGA ACTCATTCCA AGTGACCAGC TTGTCGCTGT GATCTCAGGC 
CGAAATCGCC ACGTCCGACT TTTTCCTATG TCAGCCTTGG ATGGGCGAGA GACGGATTTT 
TATAAACTGG CAGAGACTAA AGGGTGTCAG ACCATAGCTG CTGGGAAAGT GCGCCACGGA 
GCTCTGTCCT GCCTGTGTGT GGCCATGAAG AGACAGGTCC TCTGCTATGA ACTGTTCCAA 
AGCAAGACCA GGCACAGGAA GTTTAAGGAG ATTCAAGTCC CATGTAATGT CCAGTGGATG 
GCCATCTTCA GTGAGCATCT CTGTGTGGGA TTCCAGTCAG GATTTCTCAG ATACCCCCTG 
AATGGAGAAG GAGGTCCATG CAATATGCTC CATTCAAATG ACCATACACT GTCATTCATT 
TCACACCAAC CAATGGACGC TCTCTGTGCA GTTGAGATCT CCAACAAAGA GTACTTGCTG 
TGTTTTAACA GCATTGGGAT ATACACAGAC TGCCAGGGCC GAAGATCCAG ACAGCAGGAA 
CTGATGTGGC CAGCAAATCC TTCCTCCTGT TGTTACAATG CTCCATATCT CTCCGTGTAT 
AGTGAAAACG CAGTTGATAT CTTTGATGTA AACTCCATGG AATGGATACA GACTCTTCCT 
CTCAAAAAGG TCCGACCTTT AAACACTGAA GGATCATTAA ATCTCTTAGG ATTGGAGACC 
ATTAGATTAA TATATTTCAA AAATAAGATG GCAGAAGGGG ATGAACTTGT GGTTCCTGAA 
ACATCAGATA ATAGTCGGAA ACAAATGGTT AGAAATATCA ACAACAAACG GCGTTACTCC 
TTCCGAGTCC CTGAAGAAGA GAGGATGCAA CAGAGGAGGG AGATGCTACG AGATCCAGAA 
ATGAGAAATA AATTAATTTC TAACCCAACT AATTTTAACC ACATAGCACA CATGGGTCCT 
GGAGATGGAA TACAGATCCT AAAAGACCTG CCCATGCCAG GTTTCCCTTA TTCACCCCCT 
CATCACCACT CCGGCCTGAT CTCCTCTCCG AGCAACTTTG AGCACATCTA TCACATGACT 
GTCACTTCTG CTGAACAGTT TCTCTCTCCT GAGTCCATAA GCCCTGGGTC CCCCCCGTCC 
CTGCGCTCTG CCCCTGGCAC ACCACACTCT GTGACTCTGA GGAACCCACG GCCTCAGGAA 
AGCCGGACAG TATTCAGTGG CTCCGTCAGT ATTCCCTCCA TCACCAAGTC CCGCCCTGAG 
CCAGGCCGCT CCATGAGTGC CAGCAGCGGC CTGTCTGCAC GGTCGTCAGC CCAGAATGGC 
AGTGCACTAA AGAGGGAATT CTCTGGAGGA AGCTATAACA CAAAGCGACA GCCCATGCCC 
TCTCCCTCGG AGGGCTCTTT GTCATCAGGA GGCATGGACC AAGGAAGTGA CGCCCCTGCG 
AGGGACTATG ATGGAGAGGA CTCTGATTCT CCAAGGCATT CCACAGCTTC CAACAGCTCT 
AACCTGAGCA GCCCCCCAAG TCCCATTTCA CCCCAAAAGA CCAAGAGCCT CTCCCTGGAG 
AGCACAGACC GCGGGAGCTG GGATCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_006496812.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496875.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X18, mRNA.

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CloneID	OMu28732
Clone ID Related Accession (Same CDS sequence)	NM_001359545.1 , XM_006496812.3
Accession Version	XM_006496812.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496812.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGCTA GCAAAGGCAG ACGTATTGCA GACTGTGCTC CACTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG CCCGGCTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAT 
CAATTTTTCG TGAAGTCTTT CACTGCCCCT ACCAAGTGTC ACCAGTGCAC CTCCTTGATG 
GTCGGTTTGA TAAGACAGGG CTGTTCCTGT GAAGTCTGTG GGTTCTCATG TCATATAACT 
TGTGTGAACA AAGCCCCGAC GGTTTGCCCA GTTCCTCCTG AGCAAACTAA AGGTCCACTG 
GGCATAGACC CCCAGAAGGG AGTTGGGACG GCGTATGAGG GTCATGTCAG GATCCCTAAG 
CCGGCGGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTG TGGTCTGCGA CTTCAAACTG 
TTTCTTTATG ATATCGCAGA GGGAAAAGCA TCGCAGCCCA CCTCCGTCAT TAGTCAAGTG 
ATTGACATGA GAGATGAAGA ATTTTCTGTG AGTTCAGTCT TAGCTTCTGA TGTTATTCAC 
GCAAGTCGAA AAGATATCCC CTGTATATTC AGGGTCACAG CTTCCCAGCT CTCAGCACCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCA GACAGCGAGA ATGAGAGGAG TAAGTGGGTG 
GGAGTGCTGA GTGAACTCCA CAAGATTCTG AAGAAAAACA AGTTCAGAGA CCGCTCAGTG 
TATGTCCCCA AGGAGGCGTA CGACAGCACG CTACCCCTCA TTAAGACAAC GCAGGCCGCT 
GCAATCATAG ATCATGAACG GATAGCTTTG GGAAATGAAG AAGGATTATT TGTTGTGCAT 
GTCACCAAAG ATGAGATTGT TAGAGTTGGT GATAATAAGA AAATCCATCA GATCGAACTC 
ATTCCAAGTG ACCAGCTTGT CGCTGTGATC TCAGGCCGAA ATCGCCACGT CCGACTTTTT 
CCTATGTCAG CCTTGGATGG GCGAGAGACG GATTTTTATA AACTGGCAGA GACTAAAGGG 
TGTCAGACCA TAGCTGCTGG GAAAGTGCGC CACGGAGCTC TGTCCTGCCT GTGTGTGGCC 
ATGAAGAGAC AGGTCCTCTG CTATGAACTG TTCCAAAGCA AGACCAGGCA CAGGAAGTTT 
AAGGAGATTC AAGTCCCATG TAATGTCCAG TGGATGGCCA TCTTCAGTGA GCATCTCTGT 
GTGGGATTCC AGTCAGGATT TCTCAGATAC CCCCTGAATG GAGAAGGAGG TCCATGCAAT 
ATGCTCCATT CAAATGACCA TACACTGTCA TTCATTTCAC ACCAACCAAT GGACGCTCTC 
TGTGCAGTTG AGATCTCCAA CAAAGAGTAC TTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACAGACTGCC AGGGCCGAAG ATCCAGACAG CAGGAACTGA TGTGGCCAGC AAATCCTTCC 
TCCTGTTGTT ACAATGCTCC ATATCTCTCC GTGTATAGTG AAAACGCAGT TGATATCTTT 
GATGTAAACT CCATGGAATG GATACAGACT CTTCCTCTCA AAAAGGTCCG ACCTTTAAAC 
ACTGAAGGAT CATTAAATCT CTTAGGATTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTTGTGGTT CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ATATCAACAA CAAACGGCGT TACTCCTTCC GAGTCCCTGA AGAAGAGAGG 
ATGCAACAGA GGAGGGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGAACCCACG GCCTCAGGAA AGCCGGACAG TATTCAGTGG CTCCGTCAGT 
ATTCCCTCCA TCACCAAGTC CCGCCCTGAG CCAGGCCGCT CCATGAGTGC CAGCAGCGGC 
CTGTCTGCAC GGTCGTCAGC CCAGAATGGC AGTGCACTAA AGAGGGAATT CTCTGGAGGA 
AGCTATAACA CAAAGCGACA GCCCATGCCC TCTCCCTCGG AGGGCTCTTT GTCATCAGGA 
GGCATGGACC AAGGAAGTGA CGCCCCTGCG AGGGACTATG ATGGAGAGGA CTCTGATTCT 
CCAAGGCATT CCACAGCTTC CAACAGCTCT AACCTGAGCA GCCCCCCAAG TCCCATTTCA 
CCCCAAAAGA CCAAGAGCCT CTCCCTGGAG AGCACAGACC GCGGGAGCTG GGATCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496875.1
CDS	1141..6240
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006496812.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496812.3

ORF Insert Sequence:

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 
AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 
TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 
TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 
CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 
CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 
CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 
TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 
CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 
CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 
GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 
TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 
GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 
ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 
AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 
CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 
AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 
CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 
CAGCAGGAAA GAGAAGAACT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA TGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGACTAAC AGAATTGCAC ACCCAAAAGC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG CTGAGAGCTT AAGGCAGGAG 
CTGCGCAGAG CAGAAAGAGC CAAGAAAGAG CTGGAAGTTC ACACCGAAGC TCTAATTGCT 
GAAGCATCAA AGGACAAGAA GCTCCGAGAG CAGAGCGAAC ATTATTCTAA GCAACTGGAA 
AACGAGTTGG AGGGCCTGAA GCAAAAACAA ATTAGTTACT CACCAGGAAT ATGCAGCATA 
GAACATCAGC AAGAGATAAC TAAACTGAAA ACTGACTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAA TTTCTAAAAG AGAAGGAATT CACGCAAGTG AGATTAAAAA CCTGAAGAAG 
GAGCTGCATG ACTCAGAAGG CCAGCAGCTT GCTCTCAACA AAGAGATTCT GGTTTTAAAA 
GACAAATTGG AAAAAACCAG GAGAGAAAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAACAAC AATATGAACG GGAAAAAGTA TTATTAACTG AAGAGAATAA AAAGTTAACG 
AGTGAACTCG ATAAGCTGAC TTCACTGTAT GAGAGCTTAA GTTTGCGCAA CCAGCACTTA 
GAAGAAGAAG TCAAAGATCT AGCAGATAAG AAGGAATCAG TTGCCCACTG GGAAGCCCAA 
ATCACAGAGA TAATTCAGTG GGTGAGCGAT GAGAAGGATG CACGAGGCTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAGTTGGAA GCATTAAGAA ATTCCAGCTT GGGGACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAC TGGACATGTC GGCTAGACTA 
GAGTTGCAGT CTGCTCTGGA TGCAGAAATA AGAGCTAAGC AGGCCATCCA GGAAGAGCTG 
AATAAAGTCA AAGCATCTAA CATCTTAACA GAATGTAAAC TGAAAGATTC AGAGAAGAAG 
AACTTGGAAC TGTTGTCCGA AATCGAGCAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGCTA GCAAAGGCAG ACGTATTGCA GACTGTGCTC CACTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG CCCGGCTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAT 
CAATTTTTCG TGAAGTCTTT CACTGCCCCT ACCAAGTGTC ACCAGTGCAC CTCCTTGATG 
GTCGGTTTGA TAAGACAGGG CTGTTCCTGT GAAGTCTGTG GGTTCTCATG TCATATAACT 
TGTGTGAACA AAGCCCCGAC GGTTTGCCCA GTTCCTCCTG AGCAAACTAA AGGTCCACTG 
GGCATAGACC CCCAGAAGGG AGTTGGGACG GCGTATGAGG GTCATGTCAG GATCCCTAAG 
CCGGCGGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTG TGGTCTGCGA CTTCAAACTG 
TTTCTTTATG ATATCGCAGA GGGAAAAGCA TCGCAGCCCA CCTCCGTCAT TAGTCAAGTG 
ATTGACATGA GAGATGAAGA ATTTTCTGTG AGTTCAGTCT TAGCTTCTGA TGTTATTCAC 
GCAAGTCGAA AAGATATCCC CTGTATATTC AGGGTCACAG CTTCCCAGCT CTCAGCACCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCA GACAGCGAGA ATGAGAGGAG TAAGTGGGTG 
GGAGTGCTGA GTGAACTCCA CAAGATTCTG AAGAAAAACA AGTTCAGAGA CCGCTCAGTG 
TATGTCCCCA AGGAGGCGTA CGACAGCACG CTACCCCTCA TTAAGACAAC GCAGGCCGCT 
GCAATCATAG ATCATGAACG GATAGCTTTG GGAAATGAAG AAGGATTATT TGTTGTGCAT 
GTCACCAAAG ATGAGATTGT TAGAGTTGGT GATAATAAGA AAATCCATCA GATCGAACTC 
ATTCCAAGTG ACCAGCTTGT CGCTGTGATC TCAGGCCGAA ATCGCCACGT CCGACTTTTT 
CCTATGTCAG CCTTGGATGG GCGAGAGACG GATTTTTATA AACTGGCAGA GACTAAAGGG 
TGTCAGACCA TAGCTGCTGG GAAAGTGCGC CACGGAGCTC TGTCCTGCCT GTGTGTGGCC 
ATGAAGAGAC AGGTCCTCTG CTATGAACTG TTCCAAAGCA AGACCAGGCA CAGGAAGTTT 
AAGGAGATTC AAGTCCCATG TAATGTCCAG TGGATGGCCA TCTTCAGTGA GCATCTCTGT 
GTGGGATTCC AGTCAGGATT TCTCAGATAC CCCCTGAATG GAGAAGGAGG TCCATGCAAT 
ATGCTCCATT CAAATGACCA TACACTGTCA TTCATTTCAC ACCAACCAAT GGACGCTCTC 
TGTGCAGTTG AGATCTCCAA CAAAGAGTAC TTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACAGACTGCC AGGGCCGAAG ATCCAGACAG CAGGAACTGA TGTGGCCAGC AAATCCTTCC 
TCCTGTTGTT ACAATGCTCC ATATCTCTCC GTGTATAGTG AAAACGCAGT TGATATCTTT 
GATGTAAACT CCATGGAATG GATACAGACT CTTCCTCTCA AAAAGGTCCG ACCTTTAAAC 
ACTGAAGGAT CATTAAATCT CTTAGGATTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTTGTGGTT CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ATATCAACAA CAAACGGCGT TACTCCTTCC GAGTCCCTGA AGAAGAGAGG 
ATGCAACAGA GGAGGGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGAACCCACG GCCTCAGGAA AGCCGGACAG TATTCAGTGG CTCCGTCAGT 
ATTCCCTCCA TCACCAAGTC CCGCCCTGAG CCAGGCCGCT CCATGAGTGC CAGCAGCGGC 
CTGTCTGCAC GGTCGTCAGC CCAGAATGGC AGTGCACTAA AGAGGGAATT CTCTGGAGGA 
AGCTATAACA CAAAGCGACA GCCCATGCCC TCTCCCTCGG AGGGCTCTTT GTCATCAGGA 
GGCATGGACC AAGGAAGTGA CGCCCCTGCG AGGGACTATG ATGGAGAGGA CTCTGATTCT 
CCAAGGCATT CCACAGCTTC CAACAGCTCT AACCTGAGCA GCCCCCCAAG TCCCATTTCA 
CCCCAAAAGA CCAAGAGCCT CTCCCTGGAG AGCACAGACC GCGGGAGCTG GGATCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_011238802.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237104.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X7, mRNA.

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CloneID	OMu73307
Clone ID Related Accession (Same CDS sequence)	XM_011238802.2
Accession Version	XM_011238802.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238802.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATTAC ATAGAGAAGA CTTTGAAATA TTAAAGGTGA TTGGTCGAGG AGCTTTTGGG 
GAGGTTGCTG TAGTTAAACT AAAAAATGCA GATAAAGTAT TCGCCATGAA AATTCTGAAC 
AAGTGGGAAA TGCTGAAAAG AGCTGAGACA GCGTGCTTTC GTGAAGAGAG GGATGTGTTG 
GTAAATGGAG ACAGTAAGTG GATAACCACT CTGCACTATG CTTTCCAGGA TGACAATAAC 
TTATACCTGG TTATGGATTA TTATGTTGGT GGGGATTTAC TTACTCTGCT CAGCAAATTT 
GAAGATAGAT TGCCTGAAGA GATGGCTCGG TTTTACTTGG CTGAGATGGT GATAGCCATT 
GACTCAGTTC ACCAGCTCCA CTATGTTCAC AGAGACATCA AACCTGACAA TATATTGATG 
GATATGAATG GACATATTCG TTTAGCAGAT TTTGGTTCTT GTCTGAAGCT GATGGAAGAT 
GGAACGGTCC AGTCCTCGGT GGCAGTTGGA ACTCCAGATT ATATTTCTCC GGAAATCCTT 
CAGGCTATGG AAGACGGAAA AGGCAGATAT GGACCAGAGT GTGACTGGTG GTCCTTGGGG 
GTCTGTATGT ATGAGATGCT TTACGGAGAA ACACCATTCT ATGCAGAGTC TCTGGTGGAG 
ACATATGGAA AAATTATGAA TCACAAAGAG CGGTTTCAGT TTCCAGCCCA AGTGACAGAC 
GTGTCCGAAA ATGCTAAAGA TCTTATTCGA AGACTCATTT GTAGTAGAGA ACATCGACTT 
GGCCAAAATG GAATAGAAGA CTTTAAGAAG CATCCATTCT TCAGTGGAAT TGATTGGGAT 
AACATTCGGA ATTGTGAAGC ACCTTACATC CCAGAAGTTA GTAGCCCGAC AGATACGTCA 
AATTTTGATG TGGATGACGA CTGTTTAAAA AATTCTGAAA CAATGCCCCC ACCAACACAT 
ACTGCATTTT CTGGCCATCA TCTACCATTT GTTGGTTTTA CATATACTAG TAGCTGTGTT 
CTTTCTGATC GGAGCTGTTT AAGAGTCACA GCAGGCCCCA CCTCATTGGA CCTTGATGTC 
AGTGTCCAGA GGACTCTAGA CAATAACTTA GCAACTGAGG CTTATGAAAG GAGAATTAAG 
CGGCTTGAGC AGGAAAAGCT TGAACTGACT AGAAAACTTC AAGAGTCAAC ACAGACTGTT 
CAAGCTCTGC AATATTCAAC TGTTGATGGT CCACTAACGG CAAGCAAAGA TTTAGAAATT 
AAAAGTCTGA AAGAAGAGAT TGAAAAACTA AGGAAACAAG TAGCAGAAGT GAATCATTTG 
GAACAACAAC TTGAAGAAGC TAATTCTGTG AGACGAGAAC TAGATGATGC TTTCAGGCAA 
ATCAAAGCTT CTGAAAAACA AATCAAAACT TTGCAGCAGG AAAGAGAAGA ACTAAATAAG 
GAACTAGTCC AGGCTAGTGA GCGATTAAAA AACCAATCCA AAGAGCTGAA AGATGCACAC 
TGTCAGAGGA AACTGGCCAT GCAGGAATTC ATGGAGATCA ATGAGCGACT AACAGAATTG 
CACACCCAAA AGCAGAAACT TGCTCGCCAT GTCCGAGATA AGGAAGAAGA GGTGGACCTG 
GTGATGCAAA AAGCTGAGAG CTTAAGGCAG GAGCTGCGCA GAGCAGAAAG AGCCAAGAAA 
GAGCTGGAAG TTCACACCGA AGCTCTAATT GCTGAAGCAT CAAAGGACAA GAAGCTCCGA 
GAGCAGAGCG AACATTATTC TAAGCAACTG GAAAACGAGT TGGAGGGCCT GAAGCAAAAA 
CAAATTAGTT ACTCACCAGG AATATGCAGC ATAGAACATC AGCAAGAGAT AACTAAACTG 
AAAACTGACT TGGAAAAGAA AAGTATCTTT TATGAAGAAG AAATTTCTAA AAGAGAAGGA 
ATTCACGCAA GTGAGATTAA AAACCTGAAG AAGGAGCTGC ATGACTCAGA AGGCCAGCAG 
CTTGCTCTCA ACAAAGAGAT TCTGGTTTTA AAAGACAAAT TGGAAAAAAC CAGGAGAGAA 
AGTCAAAGTG AAAGGGAAGA ATTTGAAAAT GAGTTCAAAC AACAATATGA ACGGGAAAAA 
GTATTATTAA CTGAAGAGAA TAAAAAGTTA ACGAGTGAAC TCGATAAGCT GACTTCACTG 
TATGAGAGCT TAAGTTTGCG CAACCAGCAC TTAGAAGAAG AAGTCAAAGA TCTAGCAGAT 
AAGAAGGAAT CAGTTGCCCA CTGGGAAGCC CAAATCACAG AGATAATTCA GTGGGTGAGC 
GATGAGAAGG ATGCACGAGG CTATCTTCAG GCCTTAGCTT CTAAAATGAC TGAAGAGTTG 
GAAGCATTAA GAAATTCCAG CTTGGGGACA CGAGCAACAG ATATGCCCTG GAAAATGCGT 
CGTTTTGCAA AACTGGACAT GTCGGCTAGA CTAGAGTTGC AGTCTGCTCT GGATGCAGAA 
ATAAGAGCTA AGCAGGCCAT CCAGGAAGAG CTGAATAAAG TCAAAGCATC TAACATCTTA 
ACAGAATGTA AACTGAAAGA TTCAGAGAAG AAGAACTTGG AACTGTTGTC CGAAATCGAG 
CAGCTGATAA AGGACACTGA AGAGCTTAGA TCTGAAAAGG GTATAGAGCA CCAAGACTCA 
CAGCATTCTT TCTTGGCATT TTTGAATACG CCTACCGATG CTCTGGACCA ATTTGAAGAT 
TCCCTTTCTT CTTCCTCATC CTCACTGATT GATTTTTTGG ATGACCGCTC TCCGTCCTGT 
ACTCCAGCTA GCAAAGGCAG ACGTATTGCA GACTGTGCTC CACTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG CCCGGCTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAT 
CAATTTTTCG TGAAGTCTTT CACTGCCCCT ACCAAGTGTC ACCAGTGCAC CTCCTTGATG 
GTCGGTTTGA TAAGACAGGG CTGTTCCTGT GAAGTCTGTG GGTTCTCATG TCATATAACT 
TGTGTGAACA AAGCCCCGAC GGTTTGCCCA GTTCCTCCTG AGCAAACTAA AGGTCCACTG 
GGCATAGACC CCCAGAAGGG AGTTGGGACG GCGTATGAGG GTCATGTCAG GATCCCTAAG 
CCGGCGGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTG TGGTCTGCGA CTTCAAACTG 
TTTCTTTATG ATATCGCAGA GGGAAAAGCA TCGCAGCCCA CCTCCGTCAT TAGTCAAGTG 
ATTGACATGA GAGATGAAGA ATTTTCTGTG AGTTCAGTCT TAGCTTCTGA TGTTATTCAC 
GCAAGTCGAA AAGATATCCC CTGTATATTC AGGGTCACAG CTTCCCAGCT CTCAGCACCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCA GACAGCGAGA ATGAGAGGAG TAAGTGGGTG 
GGAGTGCTGA GTGAACTCCA CAAGATTCTG AAGAAAAACA AGTTCAGAGA CCGCTCAGTG 
TATGTCCCCA AGGAGGCGTA CGACAGCACG CTACCCCTCA TTAAGACAAC GCAGGCCGCT 
GCAATCATAG ATCATGAACG GATAGCTTTG GGAAATGAAG AAGGATTATT TGTTGTGCAT 
GTCACCAAAG ATGAGATTGT TAGAGTTGGT GATAATAAGA AAATCCATCA GATCGAACTC 
ATTCCAAGTG ACCAGCTTGT CGCTGTGATC TCAGGCCGAA ATCGCCACGT CCGACTTTTT 
CCTATGTCAG CCTTGGATGG GCGAGAGACG GATTTTTATA AACTGGCAGA GACTAAAGGG 
TGTCAGACCA TAGCTGCTGG GAAAGTGCGC CACGGAGCTC TGTCCTGCCT GTGTGTGGCC 
ATGAAGAGAC AGGTCCTCTG CTATGAACTG TTCCAAAGCA AGACCAGGCA CAGGAAGTTT 
AAGGAGATTC AAGTCCCATG TAATGTCCAG TGGATGGCCA TCTTCAGTGA GCATCTCTGT 
GTGGGATTCC AGTCAGGATT TCTCAGATAC CCCCTGAATG GAGAAGGAGG TCCATGCAAT 
ATGCTCCATT CAAATGACCA TACACTGTCA TTCATTTCAC ACCAACCAAT GGACGCTCTC 
TGTGCAGTTG AGATCTCCAA CAAAGAGTAC TTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACAGACTGCC AGGGCCGAAG ATCCAGACAG CAGGAACTGA TGTGGCCAGC AAATCCTTCC 
TCCTGTTGTT ACAATGCTCC ATATCTCTCC GTGTATAGTG AAAACGCAGT TGATATCTTT 
GATGTAAACT CCATGGAATG GATACAGACT CTTCCTCTCA AAAAGGTCCG ACCTTTAAAC 
ACTGAAGGAT CATTAAATCT CTTAGGATTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTTGTGGTT CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ATATCAACAA CAAACGGCGT TACTCCTTCC GAGTCCCTGA AGAAGAGAGG 
ATGCAACAGA GGAGGGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGCCAGGTTT CCCTTATTCA CCCCCTCATC ACCACTCCGG CCTGATCTCC 
TCTCCGAGCA ACTTTGAGCA CATCTATCAC ATGACTGTCA CTTCTGCTGA ACAGTTTCTC 
TCTCCTGAGT CCATAAGCCC TGGGTCCCCC CCGTCCCTGC GCTCTGCCCC TGGCACACCA 
CACTCTGTGA CTCTGAGGAA CCCACGGCCT CAGGAAAGCC GGACAGTATT CAGTGGCTCC 
GTCAGTATTC CCTCCATCAC CAAGTCCCGC CCTGAGCCAG GCCGCTCCAT GAGTGCCAGC 
AGCGGCCTGT CTGCACGGTC GTCAGCCCAG AATGGCAGTG CACTAAAGAG GGAATTCTCT 
GGAGGAAGCT ATAACACAAA GCGACAGCCC ATGCCCTCTC CCTCGGAGGG CTCTTTGTCA 
TCAGGAGGCA TGGACCAAGG AAGTGACGCC CCTGCGAGGG ACTATGATGG AGAGGACTCT 
GATTCTCCAA GGCATTCCAC AGCTTCCAAC AGCTCTAACC TGAGCAGCCC CCCAAGTCCC 
ATTTCACCCC AAAAGACCAA GAGCCTCTCC CTGGAGAGCA CAGACCGCGG GAGCTGGGAT 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237104.1
CDS	320..5545
Translation	MRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSLSSSSSSLIDFLDDRSPSCTPASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_011238802.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238802.2

ORF Insert Sequence:

ATGCGATTAC ATAGAGAAGA CTTTGAAATA TTAAAGGTGA TTGGTCGAGG AGCTTTTGGG 
GAGGTTGCTG TAGTTAAACT AAAAAATGCA GATAAAGTAT TCGCCATGAA AATTCTGAAC 
AAGTGGGAAA TGCTGAAAAG AGCTGAGACA GCGTGCTTTC GTGAAGAGAG GGATGTGTTG 
GTAAATGGAG ACAGTAAGTG GATAACCACT CTGCACTATG CTTTCCAGGA TGACAATAAC 
TTATACCTGG TTATGGATTA TTATGTTGGT GGGGATTTAC TTACTCTGCT CAGCAAATTT 
GAAGATAGAT TGCCTGAAGA GATGGCTCGG TTTTACTTGG CTGAGATGGT GATAGCCATT 
GACTCAGTTC ACCAGCTCCA CTATGTTCAC AGAGACATCA AACCTGACAA TATATTGATG 
GATATGAATG GACATATTCG TTTAGCAGAT TTTGGTTCTT GTCTGAAGCT GATGGAAGAT 
GGAACGGTCC AGTCCTCGGT GGCAGTTGGA ACTCCAGATT ATATTTCTCC GGAAATCCTT 
CAGGCTATGG AAGACGGAAA AGGCAGATAT GGACCAGAGT GTGACTGGTG GTCCTTGGGG 
GTCTGTATGT ATGAGATGCT TTACGGAGAA ACACCATTCT ATGCAGAGTC TCTGGTGGAG 
ACATATGGAA AAATTATGAA TCACAAAGAG CGGTTTCAGT TTCCAGCCCA AGTGACAGAC 
GTGTCCGAAA ATGCTAAAGA TCTTATTCGA AGACTCATTT GTAGTAGAGA ACATCGACTT 
GGCCAAAATG GAATAGAAGA CTTTAAGAAG CATCCATTCT TCAGTGGAAT TGATTGGGAT 
AACATTCGGA ATTGTGAAGC ACCTTACATC CCAGAAGTTA GTAGCCCGAC AGATACGTCA 
AATTTTGATG TGGATGACGA CTGTTTAAAA AATTCTGAAA CAATGCCCCC ACCAACACAT 
ACTGCATTTT CTGGCCATCA TCTACCATTT GTTGGTTTTA CATATACTAG TAGCTGTGTT 
CTTTCTGATC GGAGCTGTTT AAGAGTCACA GCAGGCCCCA CCTCATTGGA CCTTGATGTC 
AGTGTCCAGA GGACTCTAGA CAATAACTTA GCAACTGAGG CTTATGAAAG GAGAATTAAG 
CGGCTTGAGC AGGAAAAGCT TGAACTGACT AGAAAACTTC AAGAGTCAAC ACAGACTGTT 
CAAGCTCTGC AATATTCAAC TGTTGATGGT CCACTAACGG CAAGCAAAGA TTTAGAAATT 
AAAAGTCTGA AAGAAGAGAT TGAAAAACTA AGGAAACAAG TAGCAGAAGT GAATCATTTG 
GAACAACAAC TTGAAGAAGC TAATTCTGTG AGACGAGAAC TAGATGATGC TTTCAGGCAA 
ATCAAAGCTT CTGAAAAACA AATCAAAACT TTGCAGCAGG AAAGAGAAGA ACTAAATAAG 
GAACTAGTCC AGGCTAGTGA GCGATTAAAA AACCAATCCA AAGAGCTGAA AGATGCACAC 
TGTCAGAGGA AACTGGCCAT GCAGGAATTC ATGGAGATCA ATGAGCGACT AACAGAATTG 
CACACCCAAA AGCAGAAACT TGCTCGCCAT GTCCGAGATA AGGAAGAAGA GGTGGACCTG 
GTGATGCAAA AAGCTGAGAG CTTAAGGCAG GAGCTGCGCA GAGCAGAAAG AGCCAAGAAA 
GAGCTGGAAG TTCACACCGA AGCTCTAATT GCTGAAGCAT CAAAGGACAA GAAGCTCCGA 
GAGCAGAGCG AACATTATTC TAAGCAACTG GAAAACGAGT TGGAGGGCCT GAAGCAAAAA 
CAAATTAGTT ACTCACCAGG AATATGCAGC ATAGAACATC AGCAAGAGAT AACTAAACTG 
AAAACTGACT TGGAAAAGAA AAGTATCTTT TATGAAGAAG AAATTTCTAA AAGAGAAGGA 
ATTCACGCAA GTGAGATTAA AAACCTGAAG AAGGAGCTGC ATGACTCAGA AGGCCAGCAG 
CTTGCTCTCA ACAAAGAGAT TCTGGTTTTA AAAGACAAAT TGGAAAAAAC CAGGAGAGAA 
AGTCAAAGTG AAAGGGAAGA ATTTGAAAAT GAGTTCAAAC AACAATATGA ACGGGAAAAA 
GTATTATTAA CTGAAGAGAA TAAAAAGTTA ACGAGTGAAC TCGATAAGCT GACTTCACTG 
TATGAGAGCT TAAGTTTGCG CAACCAGCAC TTAGAAGAAG AAGTCAAAGA TCTAGCAGAT 
AAGAAGGAAT CAGTTGCCCA CTGGGAAGCC CAAATCACAG AGATAATTCA GTGGGTGAGC 
GATGAGAAGG ATGCACGAGG CTATCTTCAG GCCTTAGCTT CTAAAATGAC TGAAGAGTTG 
GAAGCATTAA GAAATTCCAG CTTGGGGACA CGAGCAACAG ATATGCCCTG GAAAATGCGT 
CGTTTTGCAA AACTGGACAT GTCGGCTAGA CTAGAGTTGC AGTCTGCTCT GGATGCAGAA 
ATAAGAGCTA AGCAGGCCAT CCAGGAAGAG CTGAATAAAG TCAAAGCATC TAACATCTTA 
ACAGAATGTA AACTGAAAGA TTCAGAGAAG AAGAACTTGG AACTGTTGTC CGAAATCGAG 
CAGCTGATAA AGGACACTGA AGAGCTTAGA TCTGAAAAGG GTATAGAGCA CCAAGACTCA 
CAGCATTCTT TCTTGGCATT TTTGAATACG CCTACCGATG CTCTGGACCA ATTTGAAGAT 
TCCCTTTCTT CTTCCTCATC CTCACTGATT GATTTTTTGG ATGACCGCTC TCCGTCCTGT 
ACTCCAGCTA GCAAAGGCAG ACGTATTGCA GACTGTGCTC CACTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG CCCGGCTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAT 
CAATTTTTCG TGAAGTCTTT CACTGCCCCT ACCAAGTGTC ACCAGTGCAC CTCCTTGATG 
GTCGGTTTGA TAAGACAGGG CTGTTCCTGT GAAGTCTGTG GGTTCTCATG TCATATAACT 
TGTGTGAACA AAGCCCCGAC GGTTTGCCCA GTTCCTCCTG AGCAAACTAA AGGTCCACTG 
GGCATAGACC CCCAGAAGGG AGTTGGGACG GCGTATGAGG GTCATGTCAG GATCCCTAAG 
CCGGCGGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTG TGGTCTGCGA CTTCAAACTG 
TTTCTTTATG ATATCGCAGA GGGAAAAGCA TCGCAGCCCA CCTCCGTCAT TAGTCAAGTG 
ATTGACATGA GAGATGAAGA ATTTTCTGTG AGTTCAGTCT TAGCTTCTGA TGTTATTCAC 
GCAAGTCGAA AAGATATCCC CTGTATATTC AGGGTCACAG CTTCCCAGCT CTCAGCACCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCA GACAGCGAGA ATGAGAGGAG TAAGTGGGTG 
GGAGTGCTGA GTGAACTCCA CAAGATTCTG AAGAAAAACA AGTTCAGAGA CCGCTCAGTG 
TATGTCCCCA AGGAGGCGTA CGACAGCACG CTACCCCTCA TTAAGACAAC GCAGGCCGCT 
GCAATCATAG ATCATGAACG GATAGCTTTG GGAAATGAAG AAGGATTATT TGTTGTGCAT 
GTCACCAAAG ATGAGATTGT TAGAGTTGGT GATAATAAGA AAATCCATCA GATCGAACTC 
ATTCCAAGTG ACCAGCTTGT CGCTGTGATC TCAGGCCGAA ATCGCCACGT CCGACTTTTT 
CCTATGTCAG CCTTGGATGG GCGAGAGACG GATTTTTATA AACTGGCAGA GACTAAAGGG 
TGTCAGACCA TAGCTGCTGG GAAAGTGCGC CACGGAGCTC TGTCCTGCCT GTGTGTGGCC 
ATGAAGAGAC AGGTCCTCTG CTATGAACTG TTCCAAAGCA AGACCAGGCA CAGGAAGTTT 
AAGGAGATTC AAGTCCCATG TAATGTCCAG TGGATGGCCA TCTTCAGTGA GCATCTCTGT 
GTGGGATTCC AGTCAGGATT TCTCAGATAC CCCCTGAATG GAGAAGGAGG TCCATGCAAT 
ATGCTCCATT CAAATGACCA TACACTGTCA TTCATTTCAC ACCAACCAAT GGACGCTCTC 
TGTGCAGTTG AGATCTCCAA CAAAGAGTAC TTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACAGACTGCC AGGGCCGAAG ATCCAGACAG CAGGAACTGA TGTGGCCAGC AAATCCTTCC 
TCCTGTTGTT ACAATGCTCC ATATCTCTCC GTGTATAGTG AAAACGCAGT TGATATCTTT 
GATGTAAACT CCATGGAATG GATACAGACT CTTCCTCTCA AAAAGGTCCG ACCTTTAAAC 
ACTGAAGGAT CATTAAATCT CTTAGGATTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTTGTGGTT CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ATATCAACAA CAAACGGCGT TACTCCTTCC GAGTCCCTGA AGAAGAGAGG 
ATGCAACAGA GGAGGGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGCCAGGTTT CCCTTATTCA CCCCCTCATC ACCACTCCGG CCTGATCTCC 
TCTCCGAGCA ACTTTGAGCA CATCTATCAC ATGACTGTCA CTTCTGCTGA ACAGTTTCTC 
TCTCCTGAGT CCATAAGCCC TGGGTCCCCC CCGTCCCTGC GCTCTGCCCC TGGCACACCA 
CACTCTGTGA CTCTGAGGAA CCCACGGCCT CAGGAAAGCC GGACAGTATT CAGTGGCTCC 
GTCAGTATTC CCTCCATCAC CAAGTCCCGC CCTGAGCCAG GCCGCTCCAT GAGTGCCAGC 
AGCGGCCTGT CTGCACGGTC GTCAGCCCAG AATGGCAGTG CACTAAAGAG GGAATTCTCT 
GGAGGAAGCT ATAACACAAA GCGACAGCCC ATGCCCTCTC CCTCGGAGGG CTCTTTGTCA 
TCAGGAGGCA TGGACCAAGG AAGTGACGCC CCTGCGAGGG ACTATGATGG AGAGGACTCT 
GATTCTCCAA GGCATTCCAC AGCTTCCAAC AGCTCTAACC TGAGCAGCCC CCCAAGTCCC 
ATTTCACCCC AAAAGACCAA GAGCCTCTCC CTGGAGAGCA CAGACCGCGG GAGCTGGGAT 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_011238803.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237105.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OMu73308
Clone ID Related Accession (Same CDS sequence)	XM_017320130.2 , XM_011238803.3 , XM_011238803.2 , XM_017320130.1
Accession Version	XM_011238803.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238803.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGATG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGACTAACAG AATTGCACAC CCAAAAGCAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGCT GAGAGCTTAA GGCAGGAGCT GCGCAGAGCA 
GAAAGAGCCA AGAAAGAGCT GGAAGTTCAC ACCGAAGCTC TAATTGCTGA AGCATCAAAG 
GACAAGAAGC TCCGAGAGCA GAGCGAACAT TATTCTAAGC AACTGGAAAA CGAGTTGGAG 
GGCCTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAATAT GCAGCATAGA ACATCAGCAA 
GAGATAACTA AACTGAAAAC TGACTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAAATT 
TCTAAAAGAG AAGGAATTCA CGCAAGTGAG ATTAAAAACC TGAAGAAGGA GCTGCATGAC 
TCAGAAGGCC AGCAGCTTGC TCTCAACAAA GAGATTCTGG TTTTAAAAGA CAAATTGGAA 
AAAACCAGGA GAGAAAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAACAACAA 
TATGAACGGG AAAAAGTATT ATTAACTGAA GAGAATAAAA AGTTAACGAG TGAACTCGAT 
AAGCTGACTT CACTGTATGA GAGCTTAAGT TTGCGCAACC AGCACTTAGA AGAAGAAGTC 
AAAGATCTAG CAGATAAGAA GGAATCAGTT GCCCACTGGG AAGCCCAAAT CACAGAGATA 
ATTCAGTGGG TGAGCGATGA GAAGGATGCA CGAGGCTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AGTTGGAAGC ATTAAGAAAT TCCAGCTTGG GGACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCAAAACTG GACATGTCGG CTAGACTAGA GTTGCAGTCT 
GCTCTGGATG CAGAAATAAG AGCTAAGCAG GCCATCCAGG AAGAGCTGAA TAAAGTCAAA 
GCATCTAACA TCTTAACAGA ATGTAAACTG AAAGATTCAG AGAAGAAGAA CTTGGAACTG 
TTGTCCGAAA TCGAGCAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GACCAATTTG AAGATTCCCT TTCTTCTTCC TCATCCTCAC TGATTGATTT TTTGGATGAC 
CGCTCTCCGT CCTGTACTCC AGCTAGCAAA GGCAGACGTA TTGCAGACTG TGCTCCACTT 
CCAGCTCACA CACCAACCTT AAGGAAAAAG GGATGCCCGG CTTCAACTGG CTTTCCACCT 
AAGCGCAAGA CTCATCAATT TTTCGTGAAG TCTTTCACTG CCCCTACCAA GTGTCACCAG 
TGCACCTCCT TGATGGTCGG TTTGATAAGA CAGGGCTGTT CCTGTGAAGT CTGTGGGTTC 
TCATGTCATA TAACTTGTGT GAACAAAGCC CCGACGGTTT GCCCAGTTCC TCCTGAGCAA 
ACTAAAGGTC CACTGGGCAT AGACCCCCAG AAGGGAGTTG GGACGGCGTA TGAGGGTCAT 
GTCAGGATCC CTAAGCCGGC GGGAGTGAAG AAAGGGTGGC AGAGAGCACT GGCTGTGGTC 
TGCGACTTCA AACTGTTTCT TTATGATATC GCAGAGGGAA AAGCATCGCA GCCCACCTCC 
GTCATTAGTC AAGTGATTGA CATGAGAGAT GAAGAATTTT CTGTGAGTTC AGTCTTAGCT 
TCTGATGTTA TTCACGCAAG TCGAAAAGAT ATCCCCTGTA TATTCAGGGT CACAGCTTCC 
CAGCTCTCAG CACCTAGTAA CAAATGTTCC ATCCTGATGC TAGCAGACAG CGAGAATGAG 
AGGAGTAAGT GGGTGGGAGT GCTGAGTGAA CTCCACAAGA TTCTGAAGAA AAACAAGTTC 
AGAGACCGCT CAGTGTATGT CCCCAAGGAG GCGTACGACA GCACGCTACC CCTCATTAAG 
ACAACGCAGG CCGCTGCAAT CATAGATCAT GAACGGATAG CTTTGGGAAA TGAAGAAGGA 
TTATTTGTTG TGCATGTCAC CAAAGATGAG ATTGTTAGAG TTGGTGATAA TAAGAAAATC 
CATCAGATCG AACTCATTCC AAGTGACCAG CTTGTCGCTG TGATCTCAGG CCGAAATCGC 
CACGTCCGAC TTTTTCCTAT GTCAGCCTTG GATGGGCGAG AGACGGATTT TTATAAACTG 
GCAGAGACTA AAGGGTGTCA GACCATAGCT GCTGGGAAAG TGCGCCACGG AGCTCTGTCC 
TGCCTGTGTG TGGCCATGAA GAGACAGGTC CTCTGCTATG AACTGTTCCA AAGCAAGACC 
AGGCACAGGA AGTTTAAGGA GATTCAAGTC CCATGTAATG TCCAGTGGAT GGCCATCTTC 
AGTGAGCATC TCTGTGTGGG ATTCCAGTCA GGATTTCTCA GATACCCCCT GAATGGAGAA 
GGAGGTCCAT GCAATATGCT CCATTCAAAT GACCATACAC TGTCATTCAT TTCACACCAA 
CCAATGGACG CTCTCTGTGC AGTTGAGATC TCCAACAAAG AGTACTTGCT GTGTTTTAAC 
AGCATTGGGA TATACACAGA CTGCCAGGGC CGAAGATCCA GACAGCAGGA ACTGATGTGG 
CCAGCAAATC CTTCCTCCTG TTGTTACAAT GCTCCATATC TCTCCGTGTA TAGTGAAAAC 
GCAGTTGATA TCTTTGATGT AAACTCCATG GAATGGATAC AGACTCTTCC TCTCAAAAAG 
GTCCGACCTT TAAACACTGA AGGATCATTA AATCTCTTAG GATTGGAGAC CATTAGATTA 
ATATATTTCA AAAATAAGAT GGCAGAAGGG GATGAACTTG TGGTTCCTGA AACATCAGAT 
AATAGTCGGA AACAAATGGT TAGAAATATC AACAACAAAC GGCGTTACTC CTTCCGAGTC 
CCTGAAGAAG AGAGGATGCA ACAGAGGAGG GAGATGCTAC GAGATCCAGA AATGAGAAAT 
AAATTAATTT CTAACCCAAC TAATTTTAAC CACATAGCAC ACATGGGTCC TGGAGATGGA 
ATACAGATCC TAAAAGACCT GCCCATGCCA GGTTTCCCTT ATTCACCCCC TCATCACCAC 
TCCGGCCTGA TCTCCTCTCC GAGCAACTTT GAGCACATCT ATCACATGAC TGTCACTTCT 
GCTGAACAGT TTCTCTCTCC TGAGTCCATA AGCCCTGGGT CCCCCCCGTC CCTGCGCTCT 
GCCCCTGGCA CACCACACTC TGTGACTCTG AGGAACCCAC GGCCTCAGGA AAGCCGGACA 
GTATTCAGTG GCTCCGTCAG TATTCCCTCC ATCACCAAGT CCCGCCCTGA GCCAGGCCGC 
TCCATGAGTG CCAGCAGCGG CCTGTCTGCA CGGTCGTCAG CCCAGAATGG CAGTGCACTA 
AAGAGGGAAT TCTCTGGAGG AAGCTATAAC ACAAAGCGAC AGCCCATGCC CTCTCCCTCG 
GAGGGCTCTT TGTCATCAGG AGGCATGGAC CAAGGAAGTG ACGCCCCTGC GAGGGACTAT 
GATGGAGAGG ACTCTGATTC TCCAAGGCAT TCCACAGCTT CCAACAGCTC TAACCTGAGC 
AGCCCCCCAA GTCCCATTTC ACCCCAAAAG ACCAAGAGCC TCTCCCTGGA GAGCACAGAC 
CGCGGGAGCT GGGATCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237105.1
CDS	1612..6732
Translation	MKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSLSSSSSSLIDFLDDRSPSCTPASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_011238803.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238803.2

ORF Insert Sequence:

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGATG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGACTAACAG AATTGCACAC CCAAAAGCAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGCT GAGAGCTTAA GGCAGGAGCT GCGCAGAGCA 
GAAAGAGCCA AGAAAGAGCT GGAAGTTCAC ACCGAAGCTC TAATTGCTGA AGCATCAAAG 
GACAAGAAGC TCCGAGAGCA GAGCGAACAT TATTCTAAGC AACTGGAAAA CGAGTTGGAG 
GGCCTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAATAT GCAGCATAGA ACATCAGCAA 
GAGATAACTA AACTGAAAAC TGACTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAAATT 
TCTAAAAGAG AAGGAATTCA CGCAAGTGAG ATTAAAAACC TGAAGAAGGA GCTGCATGAC 
TCAGAAGGCC AGCAGCTTGC TCTCAACAAA GAGATTCTGG TTTTAAAAGA CAAATTGGAA 
AAAACCAGGA GAGAAAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAACAACAA 
TATGAACGGG AAAAAGTATT ATTAACTGAA GAGAATAAAA AGTTAACGAG TGAACTCGAT 
AAGCTGACTT CACTGTATGA GAGCTTAAGT TTGCGCAACC AGCACTTAGA AGAAGAAGTC 
AAAGATCTAG CAGATAAGAA GGAATCAGTT GCCCACTGGG AAGCCCAAAT CACAGAGATA 
ATTCAGTGGG TGAGCGATGA GAAGGATGCA CGAGGCTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AGTTGGAAGC ATTAAGAAAT TCCAGCTTGG GGACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCAAAACTG GACATGTCGG CTAGACTAGA GTTGCAGTCT 
GCTCTGGATG CAGAAATAAG AGCTAAGCAG GCCATCCAGG AAGAGCTGAA TAAAGTCAAA 
GCATCTAACA TCTTAACAGA ATGTAAACTG AAAGATTCAG AGAAGAAGAA CTTGGAACTG 
TTGTCCGAAA TCGAGCAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GACCAATTTG AAGATTCCCT TTCTTCTTCC TCATCCTCAC TGATTGATTT TTTGGATGAC 
CGCTCTCCGT CCTGTACTCC AGCTAGCAAA GGCAGACGTA TTGCAGACTG TGCTCCACTT 
CCAGCTCACA CACCAACCTT AAGGAAAAAG GGATGCCCGG CTTCAACTGG CTTTCCACCT 
AAGCGCAAGA CTCATCAATT TTTCGTGAAG TCTTTCACTG CCCCTACCAA GTGTCACCAG 
TGCACCTCCT TGATGGTCGG TTTGATAAGA CAGGGCTGTT CCTGTGAAGT CTGTGGGTTC 
TCATGTCATA TAACTTGTGT GAACAAAGCC CCGACGGTTT GCCCAGTTCC TCCTGAGCAA 
ACTAAAGGTC CACTGGGCAT AGACCCCCAG AAGGGAGTTG GGACGGCGTA TGAGGGTCAT 
GTCAGGATCC CTAAGCCGGC GGGAGTGAAG AAAGGGTGGC AGAGAGCACT GGCTGTGGTC 
TGCGACTTCA AACTGTTTCT TTATGATATC GCAGAGGGAA AAGCATCGCA GCCCACCTCC 
GTCATTAGTC AAGTGATTGA CATGAGAGAT GAAGAATTTT CTGTGAGTTC AGTCTTAGCT 
TCTGATGTTA TTCACGCAAG TCGAAAAGAT ATCCCCTGTA TATTCAGGGT CACAGCTTCC 
CAGCTCTCAG CACCTAGTAA CAAATGTTCC ATCCTGATGC TAGCAGACAG CGAGAATGAG 
AGGAGTAAGT GGGTGGGAGT GCTGAGTGAA CTCCACAAGA TTCTGAAGAA AAACAAGTTC 
AGAGACCGCT CAGTGTATGT CCCCAAGGAG GCGTACGACA GCACGCTACC CCTCATTAAG 
ACAACGCAGG CCGCTGCAAT CATAGATCAT GAACGGATAG CTTTGGGAAA TGAAGAAGGA 
TTATTTGTTG TGCATGTCAC CAAAGATGAG ATTGTTAGAG TTGGTGATAA TAAGAAAATC 
CATCAGATCG AACTCATTCC AAGTGACCAG CTTGTCGCTG TGATCTCAGG CCGAAATCGC 
CACGTCCGAC TTTTTCCTAT GTCAGCCTTG GATGGGCGAG AGACGGATTT TTATAAACTG 
GCAGAGACTA AAGGGTGTCA GACCATAGCT GCTGGGAAAG TGCGCCACGG AGCTCTGTCC 
TGCCTGTGTG TGGCCATGAA GAGACAGGTC CTCTGCTATG AACTGTTCCA AAGCAAGACC 
AGGCACAGGA AGTTTAAGGA GATTCAAGTC CCATGTAATG TCCAGTGGAT GGCCATCTTC 
AGTGAGCATC TCTGTGTGGG ATTCCAGTCA GGATTTCTCA GATACCCCCT GAATGGAGAA 
GGAGGTCCAT GCAATATGCT CCATTCAAAT GACCATACAC TGTCATTCAT TTCACACCAA 
CCAATGGACG CTCTCTGTGC AGTTGAGATC TCCAACAAAG AGTACTTGCT GTGTTTTAAC 
AGCATTGGGA TATACACAGA CTGCCAGGGC CGAAGATCCA GACAGCAGGA ACTGATGTGG 
CCAGCAAATC CTTCCTCCTG TTGTTACAAT GCTCCATATC TCTCCGTGTA TAGTGAAAAC 
GCAGTTGATA TCTTTGATGT AAACTCCATG GAATGGATAC AGACTCTTCC TCTCAAAAAG 
GTCCGACCTT TAAACACTGA AGGATCATTA AATCTCTTAG GATTGGAGAC CATTAGATTA 
ATATATTTCA AAAATAAGAT GGCAGAAGGG GATGAACTTG TGGTTCCTGA AACATCAGAT 
AATAGTCGGA AACAAATGGT TAGAAATATC AACAACAAAC GGCGTTACTC CTTCCGAGTC 
CCTGAAGAAG AGAGGATGCA ACAGAGGAGG GAGATGCTAC GAGATCCAGA AATGAGAAAT 
AAATTAATTT CTAACCCAAC TAATTTTAAC CACATAGCAC ACATGGGTCC TGGAGATGGA 
ATACAGATCC TAAAAGACCT GCCCATGCCA GGTTTCCCTT ATTCACCCCC TCATCACCAC 
TCCGGCCTGA TCTCCTCTCC GAGCAACTTT GAGCACATCT ATCACATGAC TGTCACTTCT 
GCTGAACAGT TTCTCTCTCC TGAGTCCATA AGCCCTGGGT CCCCCCCGTC CCTGCGCTCT 
GCCCCTGGCA CACCACACTC TGTGACTCTG AGGAACCCAC GGCCTCAGGA AAGCCGGACA 
GTATTCAGTG GCTCCGTCAG TATTCCCTCC ATCACCAAGT CCCGCCCTGA GCCAGGCCGC 
TCCATGAGTG CCAGCAGCGG CCTGTCTGCA CGGTCGTCAG CCCAGAATGG CAGTGCACTA 
AAGAGGGAAT TCTCTGGAGG AAGCTATAAC ACAAAGCGAC AGCCCATGCC CTCTCCCTCG 
GAGGGCTCTT TGTCATCAGG AGGCATGGAC CAAGGAAGTG ACGCCCCTGC GAGGGACTAT 
GATGGAGAGG ACTCTGATTC TCCAAGGCAT TCCACAGCTT CCAACAGCTC TAACCTGAGC 
AGCCCCCCAA GTCCCATTTC ACCCCAAAAG ACCAAGAGCC TCTCCCTGGA GAGCACAGAC 
CGCGGGAGCT GGGATCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_017320130.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175619.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X16, mRNA.

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CloneID	OMu73308
Clone ID Related Accession (Same CDS sequence)	XM_017320130.2 , XM_011238803.3 , XM_011238803.2 , XM_017320130.1
Accession Version	XM_017320130.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGATG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGACTAACAG AATTGCACAC CCAAAAGCAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGCT GAGAGCTTAA GGCAGGAGCT GCGCAGAGCA 
GAAAGAGCCA AGAAAGAGCT GGAAGTTCAC ACCGAAGCTC TAATTGCTGA AGCATCAAAG 
GACAAGAAGC TCCGAGAGCA GAGCGAACAT TATTCTAAGC AACTGGAAAA CGAGTTGGAG 
GGCCTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAATAT GCAGCATAGA ACATCAGCAA 
GAGATAACTA AACTGAAAAC TGACTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAAATT 
TCTAAAAGAG AAGGAATTCA CGCAAGTGAG ATTAAAAACC TGAAGAAGGA GCTGCATGAC 
TCAGAAGGCC AGCAGCTTGC TCTCAACAAA GAGATTCTGG TTTTAAAAGA CAAATTGGAA 
AAAACCAGGA GAGAAAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAACAACAA 
TATGAACGGG AAAAAGTATT ATTAACTGAA GAGAATAAAA AGTTAACGAG TGAACTCGAT 
AAGCTGACTT CACTGTATGA GAGCTTAAGT TTGCGCAACC AGCACTTAGA AGAAGAAGTC 
AAAGATCTAG CAGATAAGAA GGAATCAGTT GCCCACTGGG AAGCCCAAAT CACAGAGATA 
ATTCAGTGGG TGAGCGATGA GAAGGATGCA CGAGGCTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AGTTGGAAGC ATTAAGAAAT TCCAGCTTGG GGACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCAAAACTG GACATGTCGG CTAGACTAGA GTTGCAGTCT 
GCTCTGGATG CAGAAATAAG AGCTAAGCAG GCCATCCAGG AAGAGCTGAA TAAAGTCAAA 
GCATCTAACA TCTTAACAGA ATGTAAACTG AAAGATTCAG AGAAGAAGAA CTTGGAACTG 
TTGTCCGAAA TCGAGCAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GACCAATTTG AAGATTCCCT TTCTTCTTCC TCATCCTCAC TGATTGATTT TTTGGATGAC 
CGCTCTCCGT CCTGTACTCC AGCTAGCAAA GGCAGACGTA TTGCAGACTG TGCTCCACTT 
CCAGCTCACA CACCAACCTT AAGGAAAAAG GGATGCCCGG CTTCAACTGG CTTTCCACCT 
AAGCGCAAGA CTCATCAATT TTTCGTGAAG TCTTTCACTG CCCCTACCAA GTGTCACCAG 
TGCACCTCCT TGATGGTCGG TTTGATAAGA CAGGGCTGTT CCTGTGAAGT CTGTGGGTTC 
TCATGTCATA TAACTTGTGT GAACAAAGCC CCGACGGTTT GCCCAGTTCC TCCTGAGCAA 
ACTAAAGGTC CACTGGGCAT AGACCCCCAG AAGGGAGTTG GGACGGCGTA TGAGGGTCAT 
GTCAGGATCC CTAAGCCGGC GGGAGTGAAG AAAGGGTGGC AGAGAGCACT GGCTGTGGTC 
TGCGACTTCA AACTGTTTCT TTATGATATC GCAGAGGGAA AAGCATCGCA GCCCACCTCC 
GTCATTAGTC AAGTGATTGA CATGAGAGAT GAAGAATTTT CTGTGAGTTC AGTCTTAGCT 
TCTGATGTTA TTCACGCAAG TCGAAAAGAT ATCCCCTGTA TATTCAGGGT CACAGCTTCC 
CAGCTCTCAG CACCTAGTAA CAAATGTTCC ATCCTGATGC TAGCAGACAG CGAGAATGAG 
AGGAGTAAGT GGGTGGGAGT GCTGAGTGAA CTCCACAAGA TTCTGAAGAA AAACAAGTTC 
AGAGACCGCT CAGTGTATGT CCCCAAGGAG GCGTACGACA GCACGCTACC CCTCATTAAG 
ACAACGCAGG CCGCTGCAAT CATAGATCAT GAACGGATAG CTTTGGGAAA TGAAGAAGGA 
TTATTTGTTG TGCATGTCAC CAAAGATGAG ATTGTTAGAG TTGGTGATAA TAAGAAAATC 
CATCAGATCG AACTCATTCC AAGTGACCAG CTTGTCGCTG TGATCTCAGG CCGAAATCGC 
CACGTCCGAC TTTTTCCTAT GTCAGCCTTG GATGGGCGAG AGACGGATTT TTATAAACTG 
GCAGAGACTA AAGGGTGTCA GACCATAGCT GCTGGGAAAG TGCGCCACGG AGCTCTGTCC 
TGCCTGTGTG TGGCCATGAA GAGACAGGTC CTCTGCTATG AACTGTTCCA AAGCAAGACC 
AGGCACAGGA AGTTTAAGGA GATTCAAGTC CCATGTAATG TCCAGTGGAT GGCCATCTTC 
AGTGAGCATC TCTGTGTGGG ATTCCAGTCA GGATTTCTCA GATACCCCCT GAATGGAGAA 
GGAGGTCCAT GCAATATGCT CCATTCAAAT GACCATACAC TGTCATTCAT TTCACACCAA 
CCAATGGACG CTCTCTGTGC AGTTGAGATC TCCAACAAAG AGTACTTGCT GTGTTTTAAC 
AGCATTGGGA TATACACAGA CTGCCAGGGC CGAAGATCCA GACAGCAGGA ACTGATGTGG 
CCAGCAAATC CTTCCTCCTG TTGTTACAAT GCTCCATATC TCTCCGTGTA TAGTGAAAAC 
GCAGTTGATA TCTTTGATGT AAACTCCATG GAATGGATAC AGACTCTTCC TCTCAAAAAG 
GTCCGACCTT TAAACACTGA AGGATCATTA AATCTCTTAG GATTGGAGAC CATTAGATTA 
ATATATTTCA AAAATAAGAT GGCAGAAGGG GATGAACTTG TGGTTCCTGA AACATCAGAT 
AATAGTCGGA AACAAATGGT TAGAAATATC AACAACAAAC GGCGTTACTC CTTCCGAGTC 
CCTGAAGAAG AGAGGATGCA ACAGAGGAGG GAGATGCTAC GAGATCCAGA AATGAGAAAT 
AAATTAATTT CTAACCCAAC TAATTTTAAC CACATAGCAC ACATGGGTCC TGGAGATGGA 
ATACAGATCC TAAAAGACCT GCCCATGCCA GGTTTCCCTT ATTCACCCCC TCATCACCAC 
TCCGGCCTGA TCTCCTCTCC GAGCAACTTT GAGCACATCT ATCACATGAC TGTCACTTCT 
GCTGAACAGT TTCTCTCTCC TGAGTCCATA AGCCCTGGGT CCCCCCCGTC CCTGCGCTCT 
GCCCCTGGCA CACCACACTC TGTGACTCTG AGGAACCCAC GGCCTCAGGA AAGCCGGACA 
GTATTCAGTG GCTCCGTCAG TATTCCCTCC ATCACCAAGT CCCGCCCTGA GCCAGGCCGC 
TCCATGAGTG CCAGCAGCGG CCTGTCTGCA CGGTCGTCAG CCCAGAATGG CAGTGCACTA 
AAGAGGGAAT TCTCTGGAGG AAGCTATAAC ACAAAGCGAC AGCCCATGCC CTCTCCCTCG 
GAGGGCTCTT TGTCATCAGG AGGCATGGAC CAAGGAAGTG ACGCCCCTGC GAGGGACTAT 
GATGGAGAGG ACTCTGATTC TCCAAGGCAT TCCACAGCTT CCAACAGCTC TAACCTGAGC 
AGCCCCCCAA GTCCCATTTC ACCCCAAAAG ACCAAGAGCC TCTCCCTGGA GAGCACAGAC 
CGCGGGAGCT GGGATCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175619.1
CDS	1685..6805
Translation	MKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKELEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSLSSSSSSLIDFLDDRSPSCTPASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_017320130.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320130.1

ORF Insert Sequence:

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGATG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGACTAACAG AATTGCACAC CCAAAAGCAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGCT GAGAGCTTAA GGCAGGAGCT GCGCAGAGCA 
GAAAGAGCCA AGAAAGAGCT GGAAGTTCAC ACCGAAGCTC TAATTGCTGA AGCATCAAAG 
GACAAGAAGC TCCGAGAGCA GAGCGAACAT TATTCTAAGC AACTGGAAAA CGAGTTGGAG 
GGCCTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAATAT GCAGCATAGA ACATCAGCAA 
GAGATAACTA AACTGAAAAC TGACTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAAATT 
TCTAAAAGAG AAGGAATTCA CGCAAGTGAG ATTAAAAACC TGAAGAAGGA GCTGCATGAC 
TCAGAAGGCC AGCAGCTTGC TCTCAACAAA GAGATTCTGG TTTTAAAAGA CAAATTGGAA 
AAAACCAGGA GAGAAAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAACAACAA 
TATGAACGGG AAAAAGTATT ATTAACTGAA GAGAATAAAA AGTTAACGAG TGAACTCGAT 
AAGCTGACTT CACTGTATGA GAGCTTAAGT TTGCGCAACC AGCACTTAGA AGAAGAAGTC 
AAAGATCTAG CAGATAAGAA GGAATCAGTT GCCCACTGGG AAGCCCAAAT CACAGAGATA 
ATTCAGTGGG TGAGCGATGA GAAGGATGCA CGAGGCTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AGTTGGAAGC ATTAAGAAAT TCCAGCTTGG GGACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCAAAACTG GACATGTCGG CTAGACTAGA GTTGCAGTCT 
GCTCTGGATG CAGAAATAAG AGCTAAGCAG GCCATCCAGG AAGAGCTGAA TAAAGTCAAA 
GCATCTAACA TCTTAACAGA ATGTAAACTG AAAGATTCAG AGAAGAAGAA CTTGGAACTG 
TTGTCCGAAA TCGAGCAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GACCAATTTG AAGATTCCCT TTCTTCTTCC TCATCCTCAC TGATTGATTT TTTGGATGAC 
CGCTCTCCGT CCTGTACTCC AGCTAGCAAA GGCAGACGTA TTGCAGACTG TGCTCCACTT 
CCAGCTCACA CACCAACCTT AAGGAAAAAG GGATGCCCGG CTTCAACTGG CTTTCCACCT 
AAGCGCAAGA CTCATCAATT TTTCGTGAAG TCTTTCACTG CCCCTACCAA GTGTCACCAG 
TGCACCTCCT TGATGGTCGG TTTGATAAGA CAGGGCTGTT CCTGTGAAGT CTGTGGGTTC 
TCATGTCATA TAACTTGTGT GAACAAAGCC CCGACGGTTT GCCCAGTTCC TCCTGAGCAA 
ACTAAAGGTC CACTGGGCAT AGACCCCCAG AAGGGAGTTG GGACGGCGTA TGAGGGTCAT 
GTCAGGATCC CTAAGCCGGC GGGAGTGAAG AAAGGGTGGC AGAGAGCACT GGCTGTGGTC 
TGCGACTTCA AACTGTTTCT TTATGATATC GCAGAGGGAA AAGCATCGCA GCCCACCTCC 
GTCATTAGTC AAGTGATTGA CATGAGAGAT GAAGAATTTT CTGTGAGTTC AGTCTTAGCT 
TCTGATGTTA TTCACGCAAG TCGAAAAGAT ATCCCCTGTA TATTCAGGGT CACAGCTTCC 
CAGCTCTCAG CACCTAGTAA CAAATGTTCC ATCCTGATGC TAGCAGACAG CGAGAATGAG 
AGGAGTAAGT GGGTGGGAGT GCTGAGTGAA CTCCACAAGA TTCTGAAGAA AAACAAGTTC 
AGAGACCGCT CAGTGTATGT CCCCAAGGAG GCGTACGACA GCACGCTACC CCTCATTAAG 
ACAACGCAGG CCGCTGCAAT CATAGATCAT GAACGGATAG CTTTGGGAAA TGAAGAAGGA 
TTATTTGTTG TGCATGTCAC CAAAGATGAG ATTGTTAGAG TTGGTGATAA TAAGAAAATC 
CATCAGATCG AACTCATTCC AAGTGACCAG CTTGTCGCTG TGATCTCAGG CCGAAATCGC 
CACGTCCGAC TTTTTCCTAT GTCAGCCTTG GATGGGCGAG AGACGGATTT TTATAAACTG 
GCAGAGACTA AAGGGTGTCA GACCATAGCT GCTGGGAAAG TGCGCCACGG AGCTCTGTCC 
TGCCTGTGTG TGGCCATGAA GAGACAGGTC CTCTGCTATG AACTGTTCCA AAGCAAGACC 
AGGCACAGGA AGTTTAAGGA GATTCAAGTC CCATGTAATG TCCAGTGGAT GGCCATCTTC 
AGTGAGCATC TCTGTGTGGG ATTCCAGTCA GGATTTCTCA GATACCCCCT GAATGGAGAA 
GGAGGTCCAT GCAATATGCT CCATTCAAAT GACCATACAC TGTCATTCAT TTCACACCAA 
CCAATGGACG CTCTCTGTGC AGTTGAGATC TCCAACAAAG AGTACTTGCT GTGTTTTAAC 
AGCATTGGGA TATACACAGA CTGCCAGGGC CGAAGATCCA GACAGCAGGA ACTGATGTGG 
CCAGCAAATC CTTCCTCCTG TTGTTACAAT GCTCCATATC TCTCCGTGTA TAGTGAAAAC 
GCAGTTGATA TCTTTGATGT AAACTCCATG GAATGGATAC AGACTCTTCC TCTCAAAAAG 
GTCCGACCTT TAAACACTGA AGGATCATTA AATCTCTTAG GATTGGAGAC CATTAGATTA 
ATATATTTCA AAAATAAGAT GGCAGAAGGG GATGAACTTG TGGTTCCTGA AACATCAGAT 
AATAGTCGGA AACAAATGGT TAGAAATATC AACAACAAAC GGCGTTACTC CTTCCGAGTC 
CCTGAAGAAG AGAGGATGCA ACAGAGGAGG GAGATGCTAC GAGATCCAGA AATGAGAAAT 
AAATTAATTT CTAACCCAAC TAATTTTAAC CACATAGCAC ACATGGGTCC TGGAGATGGA 
ATACAGATCC TAAAAGACCT GCCCATGCCA GGTTTCCCTT ATTCACCCCC TCATCACCAC 
TCCGGCCTGA TCTCCTCTCC GAGCAACTTT GAGCACATCT ATCACATGAC TGTCACTTCT 
GCTGAACAGT TTCTCTCTCC TGAGTCCATA AGCCCTGGGT CCCCCCCGTC CCTGCGCTCT 
GCCCCTGGCA CACCACACTC TGTGACTCTG AGGAACCCAC GGCCTCAGGA AAGCCGGACA 
GTATTCAGTG GCTCCGTCAG TATTCCCTCC ATCACCAAGT CCCGCCCTGA GCCAGGCCGC 
TCCATGAGTG CCAGCAGCGG CCTGTCTGCA CGGTCGTCAG CCCAGAATGG CAGTGCACTA 
AAGAGGGAAT TCTCTGGAGG AAGCTATAAC ACAAAGCGAC AGCCCATGCC CTCTCCCTCG 
GAGGGCTCTT TGTCATCAGG AGGCATGGAC CAAGGAAGTG ACGCCCCTGC GAGGGACTAT 
GATGGAGAGG ACTCTGATTC TCCAAGGCAT TCCACAGCTT CCAACAGCTC TAACCTGAGC 
AGCCCCCCAA GTCCCATTTC ACCCCAAAAG ACCAAGAGCC TCTCCCTGGA GAGCACAGAC 
CGCGGGAGCT GGGATCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42bpa ( XM_011238804.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237106.1 Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X23, mRNA.

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CloneID	OMu73309
Clone ID Related Accession (Same CDS sequence)	XM_011238804.3 , XM_011238804.2
Accession Version	XM_011238804.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase MRCK alpha isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238804.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGCTGGA AGTTCACACC GAAGCTCTAA TTGCTGAAGC ATCAAAGGAC 
AAGAAGCTCC GAGAGCAGAG CGAACATTAT TCTAAGCAAC TGGAAAACGA GTTGGAGGGC 
CTGAAGCAAA AACAAATTAG TTACTCACCA GGAATATGCA GCATAGAACA TCAGCAAGAG 
ATAACTAAAC TGAAAACTGA CTTGGAAAAG AAAAGTATCT TTTATGAAGA AGAAATTTCT 
AAAAGAGAAG GAATTCACGC AAGTGAGATT AAAAACCTGA AGAAGGAGCT GCATGACTCA 
GAAGGCCAGC AGCTTGCTCT CAACAAAGAG ATTCTGGTTT TAAAAGACAA ATTGGAAAAA 
ACCAGGAGAG AAAGTCAAAG TGAAAGGGAA GAATTTGAAA ATGAGTTCAA ACAACAATAT 
GAACGGGAAA AAGTATTATT AACTGAAGAG AATAAAAAGT TAACGAGTGA ACTCGATAAG 
CTGACTTCAC TGTATGAGAG CTTAAGTTTG CGCAACCAGC ACTTAGAAGA AGAAGTCAAA 
GATCTAGCAG ATAAGAAGGA ATCAGTTGCC CACTGGGAAG CCCAAATCAC AGAGATAATT 
CAGTGGGTGA GCGATGAGAA GGATGCACGA GGCTATCTTC AGGCCTTAGC TTCTAAAATG 
ACTGAAGAGT TGGAAGCATT AAGAAATTCC AGCTTGGGGA CACGAGCAAC AGATATGCCC 
TGGAAAATGC GTCGTTTTGC AAAACTGGAC ATGTCGGCTA GACTAGAGTT GCAGTCTGCT 
CTGGATGCAG AAATAAGAGC TAAGCAGGCC ATCCAGGAAG AGCTGAATAA AGTCAAAGCA 
TCTAACATCT TAACAGAATG TAAACTGAAA GATTCAGAGA AGAAGAACTT GGAACTGTTG 
TCCGAAATCG AGCAGCTGAT AAAGGACACT GAAGAGCTTA GATCTGAAAA GGGTATAGAG 
CACCAAGACT CACAGCATTC TTTCTTGGCA TTTTTGAATA CGCCTACCGA TGCTCTGGAC 
CAATTTGAAG ATTCCCTTTC TTCTTCCTCA TCCTCACTGA TTGATTTTTT GGATGACCGC 
TCTCCGTCCT GTACTCCAGC TAGCAAAGGC AGACGTATTG CAGACTGTGC TCCACTTCCA 
GCTCACACAC CAACCTTAAG GAAAAAGGGA TGCCCGGCTT CAACTGGCTT TCCACCTAAG 
CGCAAGACTC ATCAATTTTT CGTGAAGTCT TTCACTGCCC CTACCAAGTG TCACCAGTGC 
ACCTCCTTGA TGGTCGGTTT GATAAGACAG GGCTGTTCCT GTGAAGTCTG TGGGTTCTCA 
TGTCATATAA CTTGTGTGAA CAAAGCCCCG ACGGTTTGCC CAGTTCCTCC TGAGCAAACT 
AAAGGTCCAC TGGGCATAGA CCCCCAGAAG GGAGTTGGGA CGGCGTATGA GGGTCATGTC 
AGGATCCCTA AGCCGGCGGG AGTGAAGAAA GGGTGGCAGA GAGCACTGGC TGTGGTCTGC 
GACTTCAAAC TGTTTCTTTA TGATATCGCA GAGGGAAAAG CATCGCAGCC CACCTCCGTC 
ATTAGTCAAG TGATTGACAT GAGAGATGAA GAATTTTCTG TGAGTTCAGT CTTAGCTTCT 
GATGTTATTC ACGCAAGTCG AAAAGATATC CCCTGTATAT TCAGGGTCAC AGCTTCCCAG 
CTCTCAGCAC CTAGTAACAA ATGTTCCATC CTGATGCTAG CAGACAGCGA GAATGAGAGG 
AGTAAGTGGG TGGGAGTGCT GAGTGAACTC CACAAGATTC TGAAGAAAAA CAAGTTCAGA 
GACCGCTCAG TGTATGTCCC CAAGGAGGCG TACGACAGCA CGCTACCCCT CATTAAGACA 
ACGCAGGCCG CTGCAATCAT AGATCATGAA CGGATAGCTT TGGGAAATGA AGAAGGATTA 
TTTGTTGTGC ATGTCACCAA AGATGAGATT GTTAGAGTTG GTGATAATAA GAAAATCCAT 
CAGATCGAAC TCATTCCAAG TGACCAGCTT GTCGCTGTGA TCTCAGGCCG AAATCGCCAC 
GTCCGACTTT TTCCTATGTC AGCCTTGGAT GGGCGAGAGA CGGATTTTTA TAAACTGGCA 
GAGACTAAAG GGTGTCAGAC CATAGCTGCT GGGAAAGTGC GCCACGGAGC TCTGTCCTGC 
CTGTGTGTGG CCATGAAGAG ACAGGTCCTC TGCTATGAAC TGTTCCAAAG CAAGACCAGG 
CACAGGAAGT TTAAGGAGAT TCAAGTCCCA TGTAATGTCC AGTGGATGGC CATCTTCAGT 
GAGCATCTCT GTGTGGGATT CCAGTCAGGA TTTCTCAGAT ACCCCCTGAA TGGAGAAGGA 
GGTCCATGCA ATATGCTCCA TTCAAATGAC CATACACTGT CATTCATTTC ACACCAACCA 
ATGGACGCTC TCTGTGCAGT TGAGATCTCC AACAAAGAGT ACTTGCTGTG TTTTAACAGC 
ATTGGGATAT ACACAGACTG CCAGGGCCGA AGATCCAGAC AGCAGGAACT GATGTGGCCA 
GCAAATCCTT CCTCCTGTTG TTACAATGCT CCATATCTCT CCGTGTATAG TGAAAACGCA 
GTTGATATCT TTGATGTAAA CTCCATGGAA TGGATACAGA CTCTTCCTCT CAAAAAGGTC 
CGACCTTTAA ACACTGAAGG ATCATTAAAT CTCTTAGGAT TGGAGACCAT TAGATTAATA 
TATTTCAAAA ATAAGATGGC AGAAGGGGAT GAACTTGTGG TTCCTGAAAC ATCAGATAAT 
AGTCGGAAAC AAATGGTTAG AAATATCAAC AACAAACGGC GTTACTCCTT CCGAGTCCCT 
GAAGAAGAGA GGATGCAACA GAGGAGGGAG ATGCTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ACCCAACTAA TTTTAACCAC ATAGCACACA TGGGTCCTGG AGATGGAATA 
CAGATCCTAA AAGACCTGCC CATGCCAGGT TTCCCTTATT CACCCCCTCA TCACCACTCC 
GGCCTGATCT CCTCTCCGAG CAACTTTGAG CACATCTATC ACATGACTGT CACTTCTGCT 
GAACAGTTTC TCTCTCCTGA GTCCATAAGC CCTGGGTCCC CCCCGTCCCT GCGCTCTGCC 
CCTGGCACAC CACACTCTGT GACTCTGAGG AACCCACGGC CTCAGGAAAG CCGGACAGTA 
TTCAGTGGCT CCGTCAGTAT TCCCTCCATC ACCAAGTCCC GCCCTGAGCC AGGCCGCTCC 
ATGAGTGCCA GCAGCGGCCT GTCTGCACGG TCGTCAGCCC AGAATGGCAG TGCACTAAAG 
AGGGAATTCT CTGGAGGAAG CTATAACACA AAGCGACAGC CCATGCCCTC TCCCTCGGAG 
GGCTCTTTGT CATCAGGAGG CATGGACCAA GGAAGTGACG CCCCTGCGAG GGACTATGAT 
GGAGAGGACT CTGATTCTCC AAGGCATTCC ACAGCTTCCA ACAGCTCTAA CCTGAGCAGC 
CCCCCAAGTC CCATTTCACC CCAAAAGACC AAGAGCCTCT CCCTGGAGAG CACAGACCGC 
GGGAGCTGGG ATCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237106.1
CDS	1612..6489
Translation	MKILNKWEMLKRAETACFREERDVLVNGDSKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIKSLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKLEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFYEEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILTECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSLSSSSSSLIDFLDDRSPSCTPASKGRRIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIVRVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDHTLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYSPPHHHSGLISSPSNFEHIYHMTVTSAEQFLSPESISPGSPPSLRSAPGTPHSVTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_011238804.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC42 binding protein kinase alpha (Cdc42bpa), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238804.2

ORF Insert Sequence:

ATGAAAATTC TGAACAAGTG GGAAATGCTG AAAAGAGCTG AGACAGCGTG CTTTCGTGAA 
GAGAGGGATG TGTTGGTAAA TGGAGACAGT AAGTGGATAA CCACTCTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTACTTACT 
CTGCTCAGCA AATTTGAAGA TAGATTGCCT GAAGAGATGG CTCGGTTTTA CTTGGCTGAG 
ATGGTGATAG CCATTGACTC AGTTCACCAG CTCCACTATG TTCACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGTTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCGGTGGCAG TTGGAACTCC AGATTATATT 
TCTCCGGAAA TCCTTCAGGC TATGGAAGAC GGAAAAGGCA GATATGGACC AGAGTGTGAC 
TGGTGGTCCT TGGGGGTCTG TATGTATGAG ATGCTTTACG GAGAAACACC ATTCTATGCA 
GAGTCTCTGG TGGAGACATA TGGAAAAATT ATGAATCACA AAGAGCGGTT TCAGTTTCCA 
GCCCAAGTGA CAGACGTGTC CGAAAATGCT AAAGATCTTA TTCGAAGACT CATTTGTAGT 
AGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAGCATCC ATTCTTCAGT 
GGAATTGATT GGGATAACAT TCGGAATTGT GAAGCACCTT ACATCCCAGA AGTTAGTAGC 
CCGACAGATA CGTCAAATTT TGATGTGGAT GACGACTGTT TAAAAAATTC TGAAACAATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CATCATCTAC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTAAGAG TCACAGCAGG CCCCACCTCA 
TTGGACCTTG ATGTCAGTGT CCAGAGGACT CTAGACAATA ACTTAGCAAC TGAGGCTTAT 
GAAAGGAGAA TTAAGCGGCT TGAGCAGGAA AAGCTTGAAC TGACTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTTCAAGC TCTGCAATAT TCAACTGTTG ATGGTCCACT AACGGCAAGC 
AAAGATTTAG AAATTAAAAG TCTGAAAGAA GAGATTGAAA AACTAAGGAA ACAAGTAGCA 
GAAGTGAATC ATTTGGAACA ACAACTTGAA GAAGCTAATT CTGTGAGACG AGAACTAGAT 
GATGCTTTCA GGCAAATCAA AGCTTCTGAA AAACAAATCA AAACTTTGCA GCAGGAAAGA 
GAAGAACTAA ATAAGCTGGA AGTTCACACC GAAGCTCTAA TTGCTGAAGC ATCAAAGGAC 
AAGAAGCTCC GAGAGCAGAG CGAACATTAT TCTAAGCAAC TGGAAAACGA GTTGGAGGGC 
CTGAAGCAAA AACAAATTAG TTACTCACCA GGAATATGCA GCATAGAACA TCAGCAAGAG 
ATAACTAAAC TGAAAACTGA CTTGGAAAAG AAAAGTATCT TTTATGAAGA AGAAATTTCT 
AAAAGAGAAG GAATTCACGC AAGTGAGATT AAAAACCTGA AGAAGGAGCT GCATGACTCA 
GAAGGCCAGC AGCTTGCTCT CAACAAAGAG ATTCTGGTTT TAAAAGACAA ATTGGAAAAA 
ACCAGGAGAG AAAGTCAAAG TGAAAGGGAA GAATTTGAAA ATGAGTTCAA ACAACAATAT 
GAACGGGAAA AAGTATTATT AACTGAAGAG AATAAAAAGT TAACGAGTGA ACTCGATAAG 
CTGACTTCAC TGTATGAGAG CTTAAGTTTG CGCAACCAGC ACTTAGAAGA AGAAGTCAAA 
GATCTAGCAG ATAAGAAGGA ATCAGTTGCC CACTGGGAAG CCCAAATCAC AGAGATAATT 
CAGTGGGTGA GCGATGAGAA GGATGCACGA GGCTATCTTC AGGCCTTAGC TTCTAAAATG 
ACTGAAGAGT TGGAAGCATT AAGAAATTCC AGCTTGGGGA CACGAGCAAC AGATATGCCC 
TGGAAAATGC GTCGTTTTGC AAAACTGGAC ATGTCGGCTA GACTAGAGTT GCAGTCTGCT 
CTGGATGCAG AAATAAGAGC TAAGCAGGCC ATCCAGGAAG AGCTGAATAA AGTCAAAGCA 
TCTAACATCT TAACAGAATG TAAACTGAAA GATTCAGAGA AGAAGAACTT GGAACTGTTG 
TCCGAAATCG AGCAGCTGAT AAAGGACACT GAAGAGCTTA GATCTGAAAA GGGTATAGAG 
CACCAAGACT CACAGCATTC TTTCTTGGCA TTTTTGAATA CGCCTACCGA TGCTCTGGAC 
CAATTTGAAG ATTCCCTTTC TTCTTCCTCA TCCTCACTGA TTGATTTTTT GGATGACCGC 
TCTCCGTCCT GTACTCCAGC TAGCAAAGGC AGACGTATTG CAGACTGTGC TCCACTTCCA 
GCTCACACAC CAACCTTAAG GAAAAAGGGA TGCCCGGCTT CAACTGGCTT TCCACCTAAG 
CGCAAGACTC ATCAATTTTT CGTGAAGTCT TTCACTGCCC CTACCAAGTG TCACCAGTGC 
ACCTCCTTGA TGGTCGGTTT GATAAGACAG GGCTGTTCCT GTGAAGTCTG TGGGTTCTCA 
TGTCATATAA CTTGTGTGAA CAAAGCCCCG ACGGTTTGCC CAGTTCCTCC TGAGCAAACT 
AAAGGTCCAC TGGGCATAGA CCCCCAGAAG GGAGTTGGGA CGGCGTATGA GGGTCATGTC 
AGGATCCCTA AGCCGGCGGG AGTGAAGAAA GGGTGGCAGA GAGCACTGGC TGTGGTCTGC 
GACTTCAAAC TGTTTCTTTA TGATATCGCA GAGGGAAAAG CATCGCAGCC CACCTCCGTC 
ATTAGTCAAG TGATTGACAT GAGAGATGAA GAATTTTCTG TGAGTTCAGT CTTAGCTTCT 
GATGTTATTC ACGCAAGTCG AAAAGATATC CCCTGTATAT TCAGGGTCAC AGCTTCCCAG 
CTCTCAGCAC CTAGTAACAA ATGTTCCATC CTGATGCTAG CAGACAGCGA GAATGAGAGG 
AGTAAGTGGG TGGGAGTGCT GAGTGAACTC CACAAGATTC TGAAGAAAAA CAAGTTCAGA 
GACCGCTCAG TGTATGTCCC CAAGGAGGCG TACGACAGCA CGCTACCCCT CATTAAGACA 
ACGCAGGCCG CTGCAATCAT AGATCATGAA CGGATAGCTT TGGGAAATGA AGAAGGATTA 
TTTGTTGTGC ATGTCACCAA AGATGAGATT GTTAGAGTTG GTGATAATAA GAAAATCCAT 
CAGATCGAAC TCATTCCAAG TGACCAGCTT GTCGCTGTGA TCTCAGGCCG AAATCGCCAC 
GTCCGACTTT TTCCTATGTC AGCCTTGGAT GGGCGAGAGA CGGATTTTTA TAAACTGGCA 
GAGACTAAAG GGTGTCAGAC CATAGCTGCT GGGAAAGTGC GCCACGGAGC TCTGTCCTGC 
CTGTGTGTGG CCATGAAGAG ACAGGTCCTC TGCTATGAAC TGTTCCAAAG CAAGACCAGG 
CACAGGAAGT TTAAGGAGAT TCAAGTCCCA TGTAATGTCC AGTGGATGGC CATCTTCAGT 
GAGCATCTCT GTGTGGGATT CCAGTCAGGA TTTCTCAGAT ACCCCCTGAA TGGAGAAGGA 
GGTCCATGCA ATATGCTCCA TTCAAATGAC CATACACTGT CATTCATTTC ACACCAACCA 
ATGGACGCTC TCTGTGCAGT TGAGATCTCC AACAAAGAGT ACTTGCTGTG TTTTAACAGC 
ATTGGGATAT ACACAGACTG CCAGGGCCGA AGATCCAGAC AGCAGGAACT GATGTGGCCA 
GCAAATCCTT CCTCCTGTTG TTACAATGCT CCATATCTCT CCGTGTATAG TGAAAACGCA 
GTTGATATCT TTGATGTAAA CTCCATGGAA TGGATACAGA CTCTTCCTCT CAAAAAGGTC 
CGACCTTTAA ACACTGAAGG ATCATTAAAT CTCTTAGGAT TGGAGACCAT TAGATTAATA 
TATTTCAAAA ATAAGATGGC AGAAGGGGAT GAACTTGTGG TTCCTGAAAC ATCAGATAAT 
AGTCGGAAAC AAATGGTTAG AAATATCAAC AACAAACGGC GTTACTCCTT CCGAGTCCCT 
GAAGAAGAGA GGATGCAACA GAGGAGGGAG ATGCTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ACCCAACTAA TTTTAACCAC ATAGCACACA TGGGTCCTGG AGATGGAATA 
CAGATCCTAA AAGACCTGCC CATGCCAGGT TTCCCTTATT CACCCCCTCA TCACCACTCC 
GGCCTGATCT CCTCTCCGAG CAACTTTGAG CACATCTATC ACATGACTGT CACTTCTGCT 
GAACAGTTTC TCTCTCCTGA GTCCATAAGC CCTGGGTCCC CCCCGTCCCT GCGCTCTGCC 
CCTGGCACAC CACACTCTGT GACTCTGAGG AACCCACGGC CTCAGGAAAG CCGGACAGTA 
TTCAGTGGCT CCGTCAGTAT TCCCTCCATC ACCAAGTCCC GCCCTGAGCC AGGCCGCTCC 
ATGAGTGCCA GCAGCGGCCT GTCTGCACGG TCGTCAGCCC AGAATGGCAG TGCACTAAAG 
AGGGAATTCT CTGGAGGAAG CTATAACACA AAGCGACAGC CCATGCCCTC TCCCTCGGAG 
GGCTCTTTGT CATCAGGAGG CATGGACCAA GGAAGTGACG CCCCTGCGAG GGACTATGAT 
GGAGAGGACT CTGATTCTCC AAGGCATTCC ACAGCTTCCA ACAGCTCTAA CCTGAGCAGC 
CCCCCAAGTC CCATTTCACC CCAAAAGACC AAGAGCCTCT CCCTGGAGAG CACAGACCGC 
GGGAGCTGGG ATCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DNA methylation in the pathophysiology of hyperphenylalaninemia in the PAH(enu2) mouse model of phenylketonuria.
Molecular genetics and metabolism119(1-2)1-7(2016 09)
Dobrowolski SF,Lyons-Weiler J,Spridik K,Vockley J,Skvorak K,Biery A

Adaptor protein LRAP25 mediates myotonic dystrophy kinase-related Cdc42-binding kinase (MRCK) regulation of LIMK1 protein in lamellipodial F-actin dynamics.
The Journal of biological chemistry289(39)26989-7003(2014 Sep)
Lee IC,Leung T,Tan I

Insm1 promotes endocrine cell differentiation by modulating the expression of a network of genes that includes Neurog3 and Ripply3.
Development (Cambridge, England)141(15)2939-49(2014 Aug)
Osipovich AB,Long Q,Manduchi E,Gangula R,Hipkens SB,Schneider J,Okubo T,Stoeckert CJ Jr,Takada S,Magnuson MA

Nuclear movement regulated by Cdc42, MRCK, myosin, and actin flow establishes MTOC polarization in migrating cells.
Cell121(3)451-63(2005 May)
Gomes ER,Jani S,Gundersen GG

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Cdc42bpa cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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