si:ch211-89p3.3 gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_017352506.2	XP_017207995.1	serine/threonine-protein kinase MRCK alpha isoform X5
XM_021468485.1	XP_021324160.1	serine/threonine-protein kinase MRCK alpha isoform X6
XM_005160751.4	XP_005160808.1	serine/threonine-protein kinase MRCK alpha isoform X8
XM_017352507.1	XP_017207996.1	serine/threonine-protein kinase MRCK alpha isoform X7
XM_021468483.1	XP_021324158.1	serine/threonine-protein kinase MRCK alpha isoform X3
XM_017352506.1	XP_017207995.1	serine/threonine-protein kinase MRCK alpha isoform X2
XM_005160751.3	XP_005160808.1	serine/threonine-protein kinase MRCK alpha isoform X5
XM_005160749.4	XP_005160806.1	serine/threonine-protein kinase MRCK alpha isoform X4
XM_005160749.3	XP_005160806.1	serine/threonine-protein kinase MRCK alpha isoform X1
XM_005160750.3	XP_005160807.1	serine/threonine-protein kinase MRCK alpha isoform X3
XM_009294768.2	XP_009293043.1	serine/threonine-protein kinase MRCK alpha isoform X4
XM_009294769.2	XP_009293044.1	serine/threonine-protein kinase MRCK alpha isoform X6
XM_017352507.2	XP_017207996.1	serine/threonine-protein kinase MRCK alpha isoform X9
NM_001045094.1	NP_001038559.1	serine/threonine-protein kinase MRCK alpha
XM_021468481.1	XP_021324156.1	serine/threonine-protein kinase MRCK alpha isoform X1
XM_021468482.1	XP_021324157.1	serine/threonine-protein kinase MRCK alpha isoform X2
XM_021468486.1	XP_021324161.1	serine/threonine-protein kinase MRCK alpha isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa44775	XM_009294769.2 Latest version!	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa150649	XM_021468481.1 Latest version!	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150650	XM_021468482.1 Latest version!	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150651	XM_021468483.1 Latest version!	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150652	XM_021468485.1 Latest version!	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150653	XM_021468486.1 Latest version!	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa56577	XM_017352506.2 Latest version!	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa56578	XM_017352507.2 Latest version!	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa33549	NM_001045094.1 Latest version!	Danio rerio CDC42 binding protein kinase alpha (DMPK-like) b (cdc42bpab), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa25024	XM_005160749.4 Latest version!	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa25025	XM_005160750.3 Latest version!	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa25026	XM_005160751.4 Latest version!	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa44774	XM_009294768.2 Latest version!	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa56577	XM_017352506.1	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa25026	XM_005160751.3	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa25024	XM_005160749.3	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa56578	XM_017352507.1	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

si:ch211-89p3.3 ( XM_009294769.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293044.1 Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa44775
Clone ID Related Accession (Same CDS sequence)	XM_009294769.2
Accession Version	XM_009294769.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase MRCK alpha isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294769.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC 
TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG 
CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC 
CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT 
GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC 
TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA 
GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC 
GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA 
GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA 
CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT 
GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG 
ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC 
CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA 
GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG 
AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG 
AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC 
AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC 
AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT 
CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG 
CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC 
CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG 
GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG 
CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC 
AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC 
AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC 
GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG 
GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC 
ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG 
GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA 
ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG 
CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC 
AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC 
CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG 
AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT 
TCTGCACTGG ATCAGTTTGA TGACTCATTT TCTTCCTCTT CATCTTCTTT AGTTGAATTT 
TGGGAAGATG TAAGTTCTCC AGCCATGTTA TTGCAGAAGT GTCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293044.1
CDS	1650..4637
Translation	MSVEVRLKKLEKLIMDGPSQSSGQCFSMETLLDILVCLYDECSSSPIRREKNVLEFLDWAKPFTSKVKQMRLHKEDFEILKVIGRGAFGEVAVVKVKNTDKVFAMKILNKWEMLKRAETACFREERDVLVNGDCQWITTLHYAFQDENNLYLVMDYYVGGDLLTLLSKFEDRLPEDMAKFYLAEMVLAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPLQVTDVSEDAKDLVRRLICSREHRLGQNGIEDFKQHPFFTGIDWDNIRTCEAPYIPEVSSPTDTSNFDVDDDCLKNCETLPPPSHTAFSGHHLPFVGFTYTSKCTLSDRGCLRESVGPAQVDAHVQRSLEESLASEAYERRIRRLEQEKTELTRKLQESTQTVQALQYPDSDAPVNANKEVEIRSLKSEIDILKKQIADSGQMEQQLENASTARRDLEDSSKVVRNLEKQLKSVTQEKDDLHKELLGLAEKVKAQGKELKDAHSQKKMAIKEFAELNEQLSDVRTQKQRLSRQFKDKEEEMEGVSQKLEALRLEIRKAERMRKELEAQAEEHAAEAQKERKVRERTEQYSKQLEEELEGMKQLKQTGPPNGAASSDQQQELMRVRAELEKKSVQYEEEMCRRETLHSSEMKNLQKELRDAEGQHLTHQKEILVLKDKLEKTRRESQVEREEFVCEYKQKYEREKAHLTDENKKLSAEVEKLNGQFEQLSSSHRQLEEEMRELADKKESVAHWEAQITEIIQWVSDEKDARGYLQALATKMTEELEGLRNTSLGARGTDMPWKMRRFAKLDMSARLELQSALDTEIRAKQSIQDELNKVKATNIATECKLQESESRNQELLGEIERLRKETEELRLRRGVKHQDSQNSFLAFLNAPTSALDQFDDSFSSSSSSLVEFWEDVSSPAMLLQKCL

Target ORF information:

RefSeq Version	XM_009294769.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294769.2

ORF Insert Sequence:

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC 
TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG 
CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC 
CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT 
GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC 
TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA 
GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC 
GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA 
GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA 
CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT 
GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG 
ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC 
CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA 
GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG 
AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG 
AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC 
AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC 
AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT 
CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG 
CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC 
CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG 
GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG 
CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC 
AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC 
AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC 
GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG 
GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC 
ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG 
GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA 
ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG 
CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC 
AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC 
CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG 
AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT 
TCTGCACTGG ATCAGTTTGA TGACTCATTT TCTTCCTCTT CATCTTCTTT AGTTGAATTT 
TGGGAAGATG TAAGTTCTCC AGCCATGTTA TTGCAGAAGT GTCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-89p3.3 ( XM_021468481.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324156.1 Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa150649
Clone ID Related Accession (Same CDS sequence)	XM_021468481.1
Accession Version	XM_021468481.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase MRCK alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGTCGTCT 
CAACCTAACG ATGAGGAGCG CTTCCAGTTC CCACTTCAGG TGACTGATGT GTCCGAGGAT 
GCTAAAGATC TTGTTCGGCG GCTGATCTGC AGCAGAGAAC ACCGGCTGGG CCAGAACGGT 
ATCGAAGACT TTAAGCAGCA CCCCTTTTTC ACAGGCATCG ACTGGGACAA CATCCGCACA 
TGCGAAGCCC CCTACATCCC TGAAGTCAGC AGTCCCACTG ACACCTCCAA CTTTGATGTG 
GATGACGACT GCCTCAAAAA CTGTGAGACG TTGCCTCCAC CTTCACACAC AGCATTTTCT 
GGACACCACC TCCCATTCGT AGGCTTCACC TACACGAGTA AATGCACGCT GTCAGATCGA 
GGGTGTTTGC GGGAATCGGT CGGACCTGCA CAGGTTGATG CTCATGTTCA GCGTAGCCTG 
GAGGAGAGCC TGGCCTCTGA AGCCTATGAG AGGAGAATTC GACGTTTAGA GCAGGAGAAA 
ACCGAACTTA CCCGTAAACT ACAGGAGTCT ACGCAAACGG TGCAAGCACT GCAGTACCCT 
GATTCAGACG CCCCAGTGAA TGCTAATAAA GAGGTAGAGA TAAGGAGTTT AAAGAGTGAG 
ATCGACATCC TGAAGAAACA GATAGCAGAC TCTGGTCAGA TGGAGCAGCA GTTGGAGAAC 
GCCAGCACAG CTAGAAGAGA CCTGGAAGAC TCGTCCAAGG TCGTCAGGAA CTTAGAGAAA 
CAGCTGAAGA GTGTCACACA AGAGAAAGAT GATCTGCACA AGGAGCTGCT GGGTTTGGCA 
GAGAAGGTGA AGGCTCAGGG GAAGGAGCTA AAGGATGCTC ACAGTCAAAA AAAGATGGCC 
ATAAAGGAGT TCGCTGAGCT GAATGAACAG CTGAGCGATG TTCGCACACA AAAGCAGCGC 
CTCAGCCGAC AGTTCAAAGA CAAGGAGGAG GAGATGGAAG GAGTGTCGCA GAAACTAGAG 
GCTCTACGGC TAGAGATACG CAAAGCCGAG AGAATGCGCA AAGAGTTGGA GGCCCAGGCT 
GAGGAACATG CAGCAGAAGC TCAGAAGGAG AGAAAGGTTC GAGAAAGAAC AGAACAGTAC 
AGCAAACAAC TAGAGGAGGA GCTCGAGGGC ATGAAGCAGT TGAAGCAGAC TGGGCCTCCT 
AACGGAGCTG CAAGCTCAGA CCAGCAGCAG GAGCTGATGC GCGTTCGGGC TGAACTGGAG 
AAGAAGAGCG TACAGTACGA GGAGGAGATG TGTCGCAGAG AGACGCTCCA CAGCAGTGAG 
ATGAAGAACC TCCAAAAAGA GCTACGGGAT GCCGAGGGTC AACACCTCAC ACATCAGAAA 
GAGATACTCG TGCTTAAAGA CAAACTGGAG AAAACTCGCA GAGAAAGTCA AGTCGAGCGA 
GAAGAATTTG TGTGCGAGTA CAAGCAGAAG TACGAGAGGG AAAAAGCCCA TCTGACAGAC 
GAAAACAAGA AGCTGAGTGC CGAAGTGGAG AAGTTGAACG GTCAGTTCGA GCAGCTGAGC 
AGCAGTCACC GGCAGCTGGA GGAGGAGATG AGAGAACTGG CTGATAAGAA GGAGAGTGTT 
GCCCACTGGG AGGCCCAGAT CACCGAGATC ATCCAGTGGG TGAGCGATGA GAAGGATGCC 
CGTGGTTACC TTCAAGCTCT GGCCACCAAA ATGACAGAGG AGCTGGAAGG GCTCAGAAAC 
ACCAGTTTGG GAGCCAGAGG AACGGACATG CCGTGGAAGA TGAGGCGTTT CGCCAAGCTG 
GACATGTCTG CGCGTCTGGA GCTGCAGTCG GCGCTGGATA CTGAGATCAG AGCCAAACAG 
TCCATCCAAG ACGAGCTTAA CAAAGTCAAA GCAACCAACA TTGCCACAGA ATGCAAATTG 
CAAGAATCCG AAAGTAGAAA CCAGGAGCTG CTGGGAGAAA TTGAGAGACT GAGGAAGGAG 
ACAGAGGAGC TAAGGCTGAG GAGAGGCGTG AAGCACCAGG ATTCCCAAAA TTCCTTTTTG 
GCTTTCCTCA ATGCGCCCAC TTCTGCACTG GATCAGTTTG ATCGCTCACC CTCTTTTGGT 
CCCGGAAGCA AAACCAGACG TCTTGACTCT GCAGATTTCA CCCCTTCCAA CACTCCATCC 
AGAGATGAAG ACGCAAGATC TCGCCGTATT TCACGCTCTC GCTCACCCTC CACCGCCAGT 
GACATGGAGC CTATAGAGTT GATTGACCAC ACATCACGTA GTCTTCAGAC CCCAACAGGC 
AGATCAGGCT ACGGGAGCTT GGGACACTCA TCACCTAAGC CCAAGGCACA CCAGTTTAGT 
GTAAAGTCCT TCAATGTTCC CACTAAATGT AACCAGTGCA CCTCTCTCAT GGTGGGACTC 
ATCCGTCAGG GCTGCACATG CGAAGCCTGC AGCTTCTCCT GTCATGTCAC TTGTGCTGAT 
AAAGCCCCTT CTGTGTGCCC CGTCGCACCA GACCAGACCA GGGGCAAACT GGGCATTGAC 
ACTCAGAGGG GCATTGGGAC TGCATATGAG GGGCATCTTA GGGTGCCGAA GCCCACTGGG 
GTGAAGAAGG GTTGGCAGAG GGTCTGGGCA GTGGTTTGTG ACTTCAAACT TTTTCTGTAT 
GAGCTTGGAG AAGGGAAAGG TGCTCAGCCT GGTGTTGTAG TAAACCAGGT CATTGACATG 
AGGGATGAAG AGTTTTCCGC CAGTTCGGTT CTGGAGTCTG ATGTCATCCA TGCTAATAGG 
AAGGATATAC CCTGTATTTT TAGGGTAACA GCATCTCAGC TGTCCGCCTC CTCCAGTCAG 
AAGACGTGTA TTCTAATTCT GGCTGACAAC GAGCAAGAAA GGACGAAGTG GGTGTGCGCT 
CTGAATGAAC TGCATCGCAT CCTGAGGAAA AACAAGCTGA AGGAGCGTTT CGTCTATGTG 
CCTAAAGAAG CCTACGACAG CACACTTCCT CTCATCAAAA CCACCCAAAC TGCTGCTATT 
ATAGATCATG AACGCGTTGC TTTGGGCAAT GAGGAGGGCC TGTTTGTCAT CCACGTCACC 
AAAGATGAAA TCATTCGTGT GGGTGATGTC AAGAAGGTGC ATCATGTAGA ACTGATGCCC 
ACGGAGCACA TACTGGCCGT CATCTCGGGC AGGAACCGAC AAGTATGTCT GGTCTCCATG 
GCGGGTCTCG ACGGGAGGGA GGTCGACAAG AATAAGGTGG CAGATACTAA AAACTGCCAG 
TTGCTGGTGT CCGGTGTGGT TCGCAATGTC ACCTGCTTCT GCCTCGCCAT TAAACGGCAG 
ATCTCATGCT TCGAAATTAA CAAGAGCAAG TCACGACACT CCCATCTCGT GGACATACAA 
GCTCCAGGAA CTGTCCAATG GATGGCGCTG TTCAACCAGC AGTTGTGTGT TGGTTACCCA 
TCAGGCTTCA TGCGCTACAG TTTGTATGGA GAAACACCTC CAGCCAGCTT ACTTCATCCA 
GATGACCCCA CACTGGCATT TATTAAAACA GACAACCTGG ATGCCCTTTG TGCTGTGGAA 
ATCTCCAATA AGGAACTGCT GCTCTGCTTC AGTAAAATAG CAGTGTATGT TGACACACAC 
GGCAGACGCT CGCGCCAGCA GGAGCTGATG TGGCCCGCGG CGCCCACCGC CTGCTGCTAT 
AACGCCCCCT ATCTGTCGGT CTACAGTGAA AATGCAGTGG ATGTGTTTGA TGTGAACTCT 
ACGGAATGGA TTCAGACCAT TCCTCTCAAA AAGGTCCGGC CATTAAACAT GGATGGCTCT 
TTGAATCTCC TGGGCCTAGA AACGGTCAGA CTCATCTACT TCAGAAACAA GACCGCAGAG 
GGGGATGAAC TGGTGGTGCC TGAAGTGTGT GATAACAGCA GAAAGCAGAT GGTGCGAAGC 
ATGAGCAACA AGAGACGATT CTCGTTTCGT ATTCCTGAGG AGGAGAGACT GCAGCAGAGG 
AGAGAAATGT TGAAAGATCC TGAGAGGAGG AACAAGCTGA TCTCAAATCC TACAAATTTT 
AACCATGTGG CTCATATGGG ACCTGGAGAT GGCATGCAGA TACTCAGAGA CCTGCCAATG 
AATGTACGTG CTCAGGACAA CCGTGCTGGA TTCAGCGGCT CCGTCAGCAT CCCCTCCATT 
ACAAAAAACA GAGCAGAACC AGGGCGATCC ATGAGCGCAA GCAGTGGCCT AGGAATGCGG 
TCGTCCTCTC AGAACGGCAG TGCTCTAAGG AGGGAGCTGT CCAGCGGCAG CTACAGCTCT 
AAACGGCAGA CCATGACTTC ACCCTCCGAG AGTTCGCTGT CATCAGGAGG AGGGATGGAC 
GTGGGGGAAG CGCCGCTCTC TCAGTTTGAC AGAGAGTGGC AGGCAGTATC GCCCTCGGAG 
AAGACCCCTG ATCTGAAAAG GGCTTTAAGG ACCCTGCTTA GTAAGATGAA GGACTCTGAT 
TCGCCGCGGC ACTCCACTGC CTCCAACAGC TCCACCTTCA GCAGCCCGCC GAGCCCCGCC 
TCCCCACACA AGACAAAGTC CCTCTCATTG GAGAGCACAG ACCGCACCGC CTGGGACACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324156.1
CDS	1641..7043
Translation	MSVEVRLKKLEKLIMDGPSQSSGQCFSMETLLDILVCLYDECSSSPIRREKNVLEFLDWAKPFTSKVKQMRLHKEDFEILKVIGRGAFGEVAVVKVKNTDKVFAMKILNKWEMLKRAETACFREERDVLVNGDCQWITTLHYAFQDENNLYLVMDYYVGGDLLTLLSKFEDRLPEDMAKFYLAEMVLAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKSSQPNDEERFQFPLQVTDVSEDAKDLVRRLICSREHRLGQNGIEDFKQHPFFTGIDWDNIRTCEAPYIPEVSSPTDTSNFDVDDDCLKNCETLPPPSHTAFSGHHLPFVGFTYTSKCTLSDRGCLRESVGPAQVDAHVQRSLEESLASEAYERRIRRLEQEKTELTRKLQESTQTVQALQYPDSDAPVNANKEVEIRSLKSEIDILKKQIADSGQMEQQLENASTARRDLEDSSKVVRNLEKQLKSVTQEKDDLHKELLGLAEKVKAQGKELKDAHSQKKMAIKEFAELNEQLSDVRTQKQRLSRQFKDKEEEMEGVSQKLEALRLEIRKAERMRKELEAQAEEHAAEAQKERKVRERTEQYSKQLEEELEGMKQLKQTGPPNGAASSDQQQELMRVRAELEKKSVQYEEEMCRRETLHSSEMKNLQKELRDAEGQHLTHQKEILVLKDKLEKTRRESQVEREEFVCEYKQKYEREKAHLTDENKKLSAEVEKLNGQFEQLSSSHRQLEEEMRELADKKESVAHWEAQITEIIQWVSDEKDARGYLQALATKMTEELEGLRNTSLGARGTDMPWKMRRFAKLDMSARLELQSALDTEIRAKQSIQDELNKVKATNIATECKLQESESRNQELLGEIERLRKETEELRLRRGVKHQDSQNSFLAFLNAPTSALDQFDRSPSFGPGSKTRRLDSADFTPSNTPSRDEDARSRRISRSRSPSTASDMEPIELIDHTSRSLQTPTGRSGYGSLGHSSPKPKAHQFSVKSFNVPTKCNQCTSLMVGLIRQGCTCEACSFSCHVTCADKAPSVCPVAPDQTRGKLGIDTQRGIGTAYEGHLRVPKPTGVKKGWQRVWAVVCDFKLFLYELGEGKGAQPGVVVNQVIDMRDEEFSASSVLESDVIHANRKDIPCIFRVTASQLSASSSQKTCILILADNEQERTKWVCALNELHRILRKNKLKERFVYVPKEAYDSTLPLIKTTQTAAIIDHERVALGNEEGLFVIHVTKDEIIRVGDVKKVHHVELMPTEHILAVISGRNRQVCLVSMAGLDGREVDKNKVADTKNCQLLVSGVVRNVTCFCLAIKRQISCFEINKSKSRHSHLVDIQAPGTVQWMALFNQQLCVGYPSGFMRYSLYGETPPASLLHPDDPTLAFIKTDNLDALCAVEISNKELLLCFSKIAVYVDTHGRRSRQQELMWPAAPTACCYNAPYLSVYSENAVDVFDVNSTEWIQTIPLKKVRPLNMDGSLNLLGLETVRLIYFRNKTAEGDELVVPEVCDNSRKQMVRSMSNKRRFSFRIPEEERLQQRREMLKDPERRNKLISNPTNFNHVAHMGPGDGMQILRDLPMNVRAQDNRAGFSGSVSIPSITKNRAEPGRSMSASSGLGMRSSSQNGSALRRELSSGSYSSKRQTMTSPSESSLSSGGGMDVGEAPLSQFDREWQAVSPSEKTPDLKRALRTLLSKMKDSDSPRHSTASNSSTFSSPPSPASPHKTKSLSLESTDRTAWDT

Target ORF information:

RefSeq Version	XM_021468481.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468481.1

ORF Insert Sequence:

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGTCGTCT 
CAACCTAACG ATGAGGAGCG CTTCCAGTTC CCACTTCAGG TGACTGATGT GTCCGAGGAT 
GCTAAAGATC TTGTTCGGCG GCTGATCTGC AGCAGAGAAC ACCGGCTGGG CCAGAACGGT 
ATCGAAGACT TTAAGCAGCA CCCCTTTTTC ACAGGCATCG ACTGGGACAA CATCCGCACA 
TGCGAAGCCC CCTACATCCC TGAAGTCAGC AGTCCCACTG ACACCTCCAA CTTTGATGTG 
GATGACGACT GCCTCAAAAA CTGTGAGACG TTGCCTCCAC CTTCACACAC AGCATTTTCT 
GGACACCACC TCCCATTCGT AGGCTTCACC TACACGAGTA AATGCACGCT GTCAGATCGA 
GGGTGTTTGC GGGAATCGGT CGGACCTGCA CAGGTTGATG CTCATGTTCA GCGTAGCCTG 
GAGGAGAGCC TGGCCTCTGA AGCCTATGAG AGGAGAATTC GACGTTTAGA GCAGGAGAAA 
ACCGAACTTA CCCGTAAACT ACAGGAGTCT ACGCAAACGG TGCAAGCACT GCAGTACCCT 
GATTCAGACG CCCCAGTGAA TGCTAATAAA GAGGTAGAGA TAAGGAGTTT AAAGAGTGAG 
ATCGACATCC TGAAGAAACA GATAGCAGAC TCTGGTCAGA TGGAGCAGCA GTTGGAGAAC 
GCCAGCACAG CTAGAAGAGA CCTGGAAGAC TCGTCCAAGG TCGTCAGGAA CTTAGAGAAA 
CAGCTGAAGA GTGTCACACA AGAGAAAGAT GATCTGCACA AGGAGCTGCT GGGTTTGGCA 
GAGAAGGTGA AGGCTCAGGG GAAGGAGCTA AAGGATGCTC ACAGTCAAAA AAAGATGGCC 
ATAAAGGAGT TCGCTGAGCT GAATGAACAG CTGAGCGATG TTCGCACACA AAAGCAGCGC 
CTCAGCCGAC AGTTCAAAGA CAAGGAGGAG GAGATGGAAG GAGTGTCGCA GAAACTAGAG 
GCTCTACGGC TAGAGATACG CAAAGCCGAG AGAATGCGCA AAGAGTTGGA GGCCCAGGCT 
GAGGAACATG CAGCAGAAGC TCAGAAGGAG AGAAAGGTTC GAGAAAGAAC AGAACAGTAC 
AGCAAACAAC TAGAGGAGGA GCTCGAGGGC ATGAAGCAGT TGAAGCAGAC TGGGCCTCCT 
AACGGAGCTG CAAGCTCAGA CCAGCAGCAG GAGCTGATGC GCGTTCGGGC TGAACTGGAG 
AAGAAGAGCG TACAGTACGA GGAGGAGATG TGTCGCAGAG AGACGCTCCA CAGCAGTGAG 
ATGAAGAACC TCCAAAAAGA GCTACGGGAT GCCGAGGGTC AACACCTCAC ACATCAGAAA 
GAGATACTCG TGCTTAAAGA CAAACTGGAG AAAACTCGCA GAGAAAGTCA AGTCGAGCGA 
GAAGAATTTG TGTGCGAGTA CAAGCAGAAG TACGAGAGGG AAAAAGCCCA TCTGACAGAC 
GAAAACAAGA AGCTGAGTGC CGAAGTGGAG AAGTTGAACG GTCAGTTCGA GCAGCTGAGC 
AGCAGTCACC GGCAGCTGGA GGAGGAGATG AGAGAACTGG CTGATAAGAA GGAGAGTGTT 
GCCCACTGGG AGGCCCAGAT CACCGAGATC ATCCAGTGGG TGAGCGATGA GAAGGATGCC 
CGTGGTTACC TTCAAGCTCT GGCCACCAAA ATGACAGAGG AGCTGGAAGG GCTCAGAAAC 
ACCAGTTTGG GAGCCAGAGG AACGGACATG CCGTGGAAGA TGAGGCGTTT CGCCAAGCTG 
GACATGTCTG CGCGTCTGGA GCTGCAGTCG GCGCTGGATA CTGAGATCAG AGCCAAACAG 
TCCATCCAAG ACGAGCTTAA CAAAGTCAAA GCAACCAACA TTGCCACAGA ATGCAAATTG 
CAAGAATCCG AAAGTAGAAA CCAGGAGCTG CTGGGAGAAA TTGAGAGACT GAGGAAGGAG 
ACAGAGGAGC TAAGGCTGAG GAGAGGCGTG AAGCACCAGG ATTCCCAAAA TTCCTTTTTG 
GCTTTCCTCA ATGCGCCCAC TTCTGCACTG GATCAGTTTG ATCGCTCACC CTCTTTTGGT 
CCCGGAAGCA AAACCAGACG TCTTGACTCT GCAGATTTCA CCCCTTCCAA CACTCCATCC 
AGAGATGAAG ACGCAAGATC TCGCCGTATT TCACGCTCTC GCTCACCCTC CACCGCCAGT 
GACATGGAGC CTATAGAGTT GATTGACCAC ACATCACGTA GTCTTCAGAC CCCAACAGGC 
AGATCAGGCT ACGGGAGCTT GGGACACTCA TCACCTAAGC CCAAGGCACA CCAGTTTAGT 
GTAAAGTCCT TCAATGTTCC CACTAAATGT AACCAGTGCA CCTCTCTCAT GGTGGGACTC 
ATCCGTCAGG GCTGCACATG CGAAGCCTGC AGCTTCTCCT GTCATGTCAC TTGTGCTGAT 
AAAGCCCCTT CTGTGTGCCC CGTCGCACCA GACCAGACCA GGGGCAAACT GGGCATTGAC 
ACTCAGAGGG GCATTGGGAC TGCATATGAG GGGCATCTTA GGGTGCCGAA GCCCACTGGG 
GTGAAGAAGG GTTGGCAGAG GGTCTGGGCA GTGGTTTGTG ACTTCAAACT TTTTCTGTAT 
GAGCTTGGAG AAGGGAAAGG TGCTCAGCCT GGTGTTGTAG TAAACCAGGT CATTGACATG 
AGGGATGAAG AGTTTTCCGC CAGTTCGGTT CTGGAGTCTG ATGTCATCCA TGCTAATAGG 
AAGGATATAC CCTGTATTTT TAGGGTAACA GCATCTCAGC TGTCCGCCTC CTCCAGTCAG 
AAGACGTGTA TTCTAATTCT GGCTGACAAC GAGCAAGAAA GGACGAAGTG GGTGTGCGCT 
CTGAATGAAC TGCATCGCAT CCTGAGGAAA AACAAGCTGA AGGAGCGTTT CGTCTATGTG 
CCTAAAGAAG CCTACGACAG CACACTTCCT CTCATCAAAA CCACCCAAAC TGCTGCTATT 
ATAGATCATG AACGCGTTGC TTTGGGCAAT GAGGAGGGCC TGTTTGTCAT CCACGTCACC 
AAAGATGAAA TCATTCGTGT GGGTGATGTC AAGAAGGTGC ATCATGTAGA ACTGATGCCC 
ACGGAGCACA TACTGGCCGT CATCTCGGGC AGGAACCGAC AAGTATGTCT GGTCTCCATG 
GCGGGTCTCG ACGGGAGGGA GGTCGACAAG AATAAGGTGG CAGATACTAA AAACTGCCAG 
TTGCTGGTGT CCGGTGTGGT TCGCAATGTC ACCTGCTTCT GCCTCGCCAT TAAACGGCAG 
ATCTCATGCT TCGAAATTAA CAAGAGCAAG TCACGACACT CCCATCTCGT GGACATACAA 
GCTCCAGGAA CTGTCCAATG GATGGCGCTG TTCAACCAGC AGTTGTGTGT TGGTTACCCA 
TCAGGCTTCA TGCGCTACAG TTTGTATGGA GAAACACCTC CAGCCAGCTT ACTTCATCCA 
GATGACCCCA CACTGGCATT TATTAAAACA GACAACCTGG ATGCCCTTTG TGCTGTGGAA 
ATCTCCAATA AGGAACTGCT GCTCTGCTTC AGTAAAATAG CAGTGTATGT TGACACACAC 
GGCAGACGCT CGCGCCAGCA GGAGCTGATG TGGCCCGCGG CGCCCACCGC CTGCTGCTAT 
AACGCCCCCT ATCTGTCGGT CTACAGTGAA AATGCAGTGG ATGTGTTTGA TGTGAACTCT 
ACGGAATGGA TTCAGACCAT TCCTCTCAAA AAGGTCCGGC CATTAAACAT GGATGGCTCT 
TTGAATCTCC TGGGCCTAGA AACGGTCAGA CTCATCTACT TCAGAAACAA GACCGCAGAG 
GGGGATGAAC TGGTGGTGCC TGAAGTGTGT GATAACAGCA GAAAGCAGAT GGTGCGAAGC 
ATGAGCAACA AGAGACGATT CTCGTTTCGT ATTCCTGAGG AGGAGAGACT GCAGCAGAGG 
AGAGAAATGT TGAAAGATCC TGAGAGGAGG AACAAGCTGA TCTCAAATCC TACAAATTTT 
AACCATGTGG CTCATATGGG ACCTGGAGAT GGCATGCAGA TACTCAGAGA CCTGCCAATG 
AATGTACGTG CTCAGGACAA CCGTGCTGGA TTCAGCGGCT CCGTCAGCAT CCCCTCCATT 
ACAAAAAACA GAGCAGAACC AGGGCGATCC ATGAGCGCAA GCAGTGGCCT AGGAATGCGG 
TCGTCCTCTC AGAACGGCAG TGCTCTAAGG AGGGAGCTGT CCAGCGGCAG CTACAGCTCT 
AAACGGCAGA CCATGACTTC ACCCTCCGAG AGTTCGCTGT CATCAGGAGG AGGGATGGAC 
GTGGGGGAAG CGCCGCTCTC TCAGTTTGAC AGAGAGTGGC AGGCAGTATC GCCCTCGGAG 
AAGACCCCTG ATCTGAAAAG GGCTTTAAGG ACCCTGCTTA GTAAGATGAA GGACTCTGAT 
TCGCCGCGGC ACTCCACTGC CTCCAACAGC TCCACCTTCA GCAGCCCGCC GAGCCCCGCC 
TCCCCACACA AGACAAAGTC CCTCTCATTG GAGAGCACAG ACCGCACCGC CTGGGACACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-89p3.3 ( XM_021468482.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324157.1 Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X2, mRNA.

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CloneID	ODa150650
Clone ID Related Accession (Same CDS sequence)	XM_021468482.1
Accession Version	XM_021468482.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase MRCK alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGTCGTCT 
CAACCTAACG ATGAGGAGCG CTTCCAGTTC CCACTTCAGG TGACTGATGT GTCCGAGGAT 
GCTAAAGATC TTGTTCGGCG GCTGATCTGC AGCAGAGAAC ACCGGCTGGG CCAGAACGGT 
ATCGAAGACT TTAAGCAGCA CCCCTTTTTC ACAGGCATCG ACTGGGACAA CATCCGCACA 
TGCGAAGCCC CCTACATCCC TGAAGTCAGC AGTCCCACTG ACACCTCCAA CTTTGATGTG 
GATGACGACT GCCTCAAAAA CTGTGAGACG TTGCCTCCAC CTTCACACAC AGCATTTTCT 
GGACACCACC TCCCATTCGT AGGCTTCACC TACACGAGTA AATGCACGCT GTCAGATCGA 
GGGTGTTTGC GGGAATCGGT CGGACCTGCA CAGGTTGATG CTCATGTTCA GCGTAGCCTG 
GAGGAGAGCC TGGCCTCTGA AGCCTATGAG AGGAGAATTC GACGTTTAGA GCAGGAGAAA 
ACCGAACTTA CCCGTAAACT ACAGGAGTCT ACGCAAACGG TGCAAGCACT GCAGTACCCT 
GATTCAGACG CCCCAGTGAA TGCTAATAAA GAGGTAGAGA TAAGGAGTTT AAAGAGTGAG 
ATCGACATCC TGAAGAAACA GATAGCAGAC TCTGGTCAGA TGGAGCAGCA GTTGGAGAAC 
GCCAGCACAG CTAGAAGAGA CCTGGAAGAC TCGTCCAAGG TCGTCAGGAA CTTAGAGAAA 
CAGCTGAAGA GTGTCACACA AGAGAAAGAT GATCTGCACA AGGAGCTGCT GGGTTTGGCA 
GAGAAGGTGA AGGCTCAGGG GAAGGAGCTA AAGGATGCTC ACAGTCAAAA AAAGATGGCC 
ATAAAGGAGT TCGCTGAGCT GAATGAACAG CTGAGCGATG TTCGCACACA AAAGCAGCGC 
CTCAGCCGAC AGTTCAAAGA CAAGGAGGAG GAGATGGAAG GAGTGTCGCA GAAACTAGAG 
GCTCTACGGC TAGAGATACG CAAAGCCGAG AGAATGCGCA AAGAGTTGGA GGCCCAGGCT 
GAGGAACATG CAGCAGAAGC TCAGAAGGAG AGAAAGGTTC GAGAAAGAAC AGAACAGTAC 
AGCAAACAAC TAGAGGAGGA GCTCGAGGGC ATGAAGTTGA AGCAGACTGG GCCTCCTAAC 
GGAGCTGCAA GCTCAGACCA GCAGCAGGAG CTGATGCGCG TTCGGGCTGA ACTGGAGAAG 
AAGAGCGTAC AGTACGAGGA GGAGATGTGT CGCAGAGAGA CGCTCCACAG CAGTGAGATG 
AAGAACCTCC AAAAAGAGCT ACGGGATGCC GAGGGTCAAC ACCTCACACA TCAGAAAGAG 
ATACTCGTGC TTAAAGACAA ACTGGAGAAA ACTCGCAGAG AAAGTCAAGT CGAGCGAGAA 
GAATTTGTGT GCGAGTACAA GCAGAAGTAC GAGAGGGAAA AAGCCCATCT GACAGACGAA 
AACAAGAAGC TGAGTGCCGA AGTGGAGAAG TTGAACGGTC AGTTCGAGCA GCTGAGCAGC 
AGTCACCGGC AGCTGGAGGA GGAGATGAGA GAACTGGCTG ATAAGAAGGA GAGTGTTGCC 
CACTGGGAGG CCCAGATCAC CGAGATCATC CAGTGGGTGA GCGATGAGAA GGATGCCCGT 
GGTTACCTTC AAGCTCTGGC CACCAAAATG ACAGAGGAGC TGGAAGGGCT CAGAAACACC 
AGTTTGGGAG CCAGAGGAAC GGACATGCCG TGGAAGATGA GGCGTTTCGC CAAGCTGGAC 
ATGTCTGCGC GTCTGGAGCT GCAGTCGGCG CTGGATACTG AGATCAGAGC CAAACAGTCC 
ATCCAAGACG AGCTTAACAA AGTCAAAGCA ACCAACATTG CCACAGAATG CAAATTGCAA 
GAATCCGAAA GTAGAAACCA GGAGCTGCTG GGAGAAATTG AGAGACTGAG GAAGGAGACA 
GAGGAGCTAA GGCTGAGGAG AGGCGTGAAG CACCAGGATT CCCAAAATTC CTTTTTGGCT 
TTCCTCAATG CGCCCACTTC TGCACTGGAT CAGTTTGATC GCTCACCCTC TTTTGGTCCC 
GGAAGCAAAA CCAGACGTCT TGACTCTGCA GATTTCACCC CTTCCAACAC TCCATCCAGA 
GATGAAGACG CAAGATCTCG CCGTATTTCA CGCTCTCGCT CACCCTCCAC CGCCAGTGAC 
ATGGAGCCTA TAGAGTTGAT TGACCACACA TCACGTAGTC TTCAGACCCC AACAGGCAGA 
TCAGGCTACG GGAGCTTGGG ACACTCATCA CCTAAGCCCA AGGCACACCA GTTTAGTGTA 
AAGTCCTTCA ATGTTCCCAC TAAATGTAAC CAGTGCACCT CTCTCATGGT GGGACTCATC 
CGTCAGGGCT GCACATGCGA AGCCTGCAGC TTCTCCTGTC ATGTCACTTG TGCTGATAAA 
GCCCCTTCTG TGTGCCCCGT CGCACCAGAC CAGACCAGGG GCAAACTGGG CATTGACACT 
CAGAGGGGCA TTGGGACTGC ATATGAGGGG CATCTTAGGG TGCCGAAGCC CACTGGGGTG 
AAGAAGGGTT GGCAGAGGGT CTGGGCAGTG GTTTGTGACT TCAAACTTTT TCTGTATGAG 
CTTGGAGAAG GGAAAGGTGC TCAGCCTGGT GTTGTAGTAA ACCAGGTCAT TGACATGAGG 
GATGAAGAGT TTTCCGCCAG TTCGGTTCTG GAGTCTGATG TCATCCATGC TAATAGGAAG 
GATATACCCT GTATTTTTAG GGTAACAGCA TCTCAGCTGT CCGCCTCCTC CAGTCAGAAG 
ACGTGTATTC TAATTCTGGC TGACAACGAG CAAGAAAGGA CGAAGTGGGT GTGCGCTCTG 
AATGAACTGC ATCGCATCCT GAGGAAAAAC AAGCTGAAGG AGCGTTTCGT CTATGTGCCT 
AAAGAAGCCT ACGACAGCAC ACTTCCTCTC ATCAAAACCA CCCAAACTGC TGCTATTATA 
GATCATGAAC GCGTTGCTTT GGGCAATGAG GAGGGCCTGT TTGTCATCCA CGTCACCAAA 
GATGAAATCA TTCGTGTGGG TGATGTCAAG AAGGTGCATC ATGTAGAACT GATGCCCACG 
GAGCACATAC TGGCCGTCAT CTCGGGCAGG AACCGACAAG TATGTCTGGT CTCCATGGCG 
GGTCTCGACG GGAGGGAGGT CGACAAGAAT AAGGTGGCAG ATACTAAAAA CTGCCAGTTG 
CTGGTGTCCG GTGTGGTTCG CAATGTCACC TGCTTCTGCC TCGCCATTAA ACGGCAGATC 
TCATGCTTCG AAATTAACAA GAGCAAGTCA CGACACTCCC ATCTCGTGGA CATACAAGCT 
CCAGGAACTG TCCAATGGAT GGCGCTGTTC AACCAGCAGT TGTGTGTTGG TTACCCATCA 
GGCTTCATGC GCTACAGTTT GTATGGAGAA ACACCTCCAG CCAGCTTACT TCATCCAGAT 
GACCCCACAC TGGCATTTAT TAAAACAGAC AACCTGGATG CCCTTTGTGC TGTGGAAATC 
TCCAATAAGG AACTGCTGCT CTGCTTCAGT AAAATAGCAG TGTATGTTGA CACACACGGC 
AGACGCTCGC GCCAGCAGGA GCTGATGTGG CCCGCGGCGC CCACCGCCTG CTGCTATAAC 
GCCCCCTATC TGTCGGTCTA CAGTGAAAAT GCAGTGGATG TGTTTGATGT GAACTCTACG 
GAATGGATTC AGACCATTCC TCTCAAAAAG GTCCGGCCAT TAAACATGGA TGGCTCTTTG 
AATCTCCTGG GCCTAGAAAC GGTCAGACTC ATCTACTTCA GAAACAAGAC CGCAGAGGGG 
GATGAACTGG TGGTGCCTGA AGTGTGTGAT AACAGCAGAA AGCAGATGGT GCGAAGCATG 
AGCAACAAGA GACGATTCTC GTTTCGTATT CCTGAGGAGG AGAGACTGCA GCAGAGGAGA 
GAAATGTTGA AAGATCCTGA GAGGAGGAAC AAGCTGATCT CAAATCCTAC AAATTTTAAC 
CATGTGGCTC ATATGGGACC TGGAGATGGC ATGCAGATAC TCAGAGACCT GCCAATGAAT 
GTACGTGCTC AGGACAACCG TGCTGGATTC AGCGGCTCCG TCAGCATCCC CTCCATTACA 
AAAAACAGAG CAGAACCAGG GCGATCCATG AGCGCAAGCA GTGGCCTAGG AATGCGGTCG 
TCCTCTCAGA ACGGCAGTGC TCTAAGGAGG GAGCTGTCCA GCGGCAGCTA CAGCTCTAAA 
CGGCAGACCA TGACTTCACC CTCCGAGAGT TCGCTGTCAT CAGGAGGAGG GATGGACGTG 
GGGGAAGCGC CGCTCTCTCA GTTTGACAGA GAGTGGCAGG CAGTATCGCC CTCGGAGAAG 
ACCCCTGATC TGAAAAGGGC TTTAAGGACC CTGCTTAGTA AGATGAAGGA CTCTGATTCG 
CCGCGGCACT CCACTGCCTC CAACAGCTCC ACCTTCAGCA GCCCGCCGAG CCCCGCCTCC 
CCACACAAGA CAAAGTCCCT CTCATTGGAG AGCACAGACC GCACCGCCTG GGACACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324157.1
CDS	1641..7040
Translation	MSVEVRLKKLEKLIMDGPSQSSGQCFSMETLLDILVCLYDECSSSPIRREKNVLEFLDWAKPFTSKVKQMRLHKEDFEILKVIGRGAFGEVAVVKVKNTDKVFAMKILNKWEMLKRAETACFREERDVLVNGDCQWITTLHYAFQDENNLYLVMDYYVGGDLLTLLSKFEDRLPEDMAKFYLAEMVLAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKSSQPNDEERFQFPLQVTDVSEDAKDLVRRLICSREHRLGQNGIEDFKQHPFFTGIDWDNIRTCEAPYIPEVSSPTDTSNFDVDDDCLKNCETLPPPSHTAFSGHHLPFVGFTYTSKCTLSDRGCLRESVGPAQVDAHVQRSLEESLASEAYERRIRRLEQEKTELTRKLQESTQTVQALQYPDSDAPVNANKEVEIRSLKSEIDILKKQIADSGQMEQQLENASTARRDLEDSSKVVRNLEKQLKSVTQEKDDLHKELLGLAEKVKAQGKELKDAHSQKKMAIKEFAELNEQLSDVRTQKQRLSRQFKDKEEEMEGVSQKLEALRLEIRKAERMRKELEAQAEEHAAEAQKERKVRERTEQYSKQLEEELEGMKLKQTGPPNGAASSDQQQELMRVRAELEKKSVQYEEEMCRRETLHSSEMKNLQKELRDAEGQHLTHQKEILVLKDKLEKTRRESQVEREEFVCEYKQKYEREKAHLTDENKKLSAEVEKLNGQFEQLSSSHRQLEEEMRELADKKESVAHWEAQITEIIQWVSDEKDARGYLQALATKMTEELEGLRNTSLGARGTDMPWKMRRFAKLDMSARLELQSALDTEIRAKQSIQDELNKVKATNIATECKLQESESRNQELLGEIERLRKETEELRLRRGVKHQDSQNSFLAFLNAPTSALDQFDRSPSFGPGSKTRRLDSADFTPSNTPSRDEDARSRRISRSRSPSTASDMEPIELIDHTSRSLQTPTGRSGYGSLGHSSPKPKAHQFSVKSFNVPTKCNQCTSLMVGLIRQGCTCEACSFSCHVTCADKAPSVCPVAPDQTRGKLGIDTQRGIGTAYEGHLRVPKPTGVKKGWQRVWAVVCDFKLFLYELGEGKGAQPGVVVNQVIDMRDEEFSASSVLESDVIHANRKDIPCIFRVTASQLSASSSQKTCILILADNEQERTKWVCALNELHRILRKNKLKERFVYVPKEAYDSTLPLIKTTQTAAIIDHERVALGNEEGLFVIHVTKDEIIRVGDVKKVHHVELMPTEHILAVISGRNRQVCLVSMAGLDGREVDKNKVADTKNCQLLVSGVVRNVTCFCLAIKRQISCFEINKSKSRHSHLVDIQAPGTVQWMALFNQQLCVGYPSGFMRYSLYGETPPASLLHPDDPTLAFIKTDNLDALCAVEISNKELLLCFSKIAVYVDTHGRRSRQQELMWPAAPTACCYNAPYLSVYSENAVDVFDVNSTEWIQTIPLKKVRPLNMDGSLNLLGLETVRLIYFRNKTAEGDELVVPEVCDNSRKQMVRSMSNKRRFSFRIPEEERLQQRREMLKDPERRNKLISNPTNFNHVAHMGPGDGMQILRDLPMNVRAQDNRAGFSGSVSIPSITKNRAEPGRSMSASSGLGMRSSSQNGSALRRELSSGSYSSKRQTMTSPSESSLSSGGGMDVGEAPLSQFDREWQAVSPSEKTPDLKRALRTLLSKMKDSDSPRHSTASNSSTFSSPPSPASPHKTKSLSLESTDRTAWDT

Target ORF information:

RefSeq Version	XM_021468482.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468482.1

ORF Insert Sequence:

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGTCGTCT 
CAACCTAACG ATGAGGAGCG CTTCCAGTTC CCACTTCAGG TGACTGATGT GTCCGAGGAT 
GCTAAAGATC TTGTTCGGCG GCTGATCTGC AGCAGAGAAC ACCGGCTGGG CCAGAACGGT 
ATCGAAGACT TTAAGCAGCA CCCCTTTTTC ACAGGCATCG ACTGGGACAA CATCCGCACA 
TGCGAAGCCC CCTACATCCC TGAAGTCAGC AGTCCCACTG ACACCTCCAA CTTTGATGTG 
GATGACGACT GCCTCAAAAA CTGTGAGACG TTGCCTCCAC CTTCACACAC AGCATTTTCT 
GGACACCACC TCCCATTCGT AGGCTTCACC TACACGAGTA AATGCACGCT GTCAGATCGA 
GGGTGTTTGC GGGAATCGGT CGGACCTGCA CAGGTTGATG CTCATGTTCA GCGTAGCCTG 
GAGGAGAGCC TGGCCTCTGA AGCCTATGAG AGGAGAATTC GACGTTTAGA GCAGGAGAAA 
ACCGAACTTA CCCGTAAACT ACAGGAGTCT ACGCAAACGG TGCAAGCACT GCAGTACCCT 
GATTCAGACG CCCCAGTGAA TGCTAATAAA GAGGTAGAGA TAAGGAGTTT AAAGAGTGAG 
ATCGACATCC TGAAGAAACA GATAGCAGAC TCTGGTCAGA TGGAGCAGCA GTTGGAGAAC 
GCCAGCACAG CTAGAAGAGA CCTGGAAGAC TCGTCCAAGG TCGTCAGGAA CTTAGAGAAA 
CAGCTGAAGA GTGTCACACA AGAGAAAGAT GATCTGCACA AGGAGCTGCT GGGTTTGGCA 
GAGAAGGTGA AGGCTCAGGG GAAGGAGCTA AAGGATGCTC ACAGTCAAAA AAAGATGGCC 
ATAAAGGAGT TCGCTGAGCT GAATGAACAG CTGAGCGATG TTCGCACACA AAAGCAGCGC 
CTCAGCCGAC AGTTCAAAGA CAAGGAGGAG GAGATGGAAG GAGTGTCGCA GAAACTAGAG 
GCTCTACGGC TAGAGATACG CAAAGCCGAG AGAATGCGCA AAGAGTTGGA GGCCCAGGCT 
GAGGAACATG CAGCAGAAGC TCAGAAGGAG AGAAAGGTTC GAGAAAGAAC AGAACAGTAC 
AGCAAACAAC TAGAGGAGGA GCTCGAGGGC ATGAAGTTGA AGCAGACTGG GCCTCCTAAC 
GGAGCTGCAA GCTCAGACCA GCAGCAGGAG CTGATGCGCG TTCGGGCTGA ACTGGAGAAG 
AAGAGCGTAC AGTACGAGGA GGAGATGTGT CGCAGAGAGA CGCTCCACAG CAGTGAGATG 
AAGAACCTCC AAAAAGAGCT ACGGGATGCC GAGGGTCAAC ACCTCACACA TCAGAAAGAG 
ATACTCGTGC TTAAAGACAA ACTGGAGAAA ACTCGCAGAG AAAGTCAAGT CGAGCGAGAA 
GAATTTGTGT GCGAGTACAA GCAGAAGTAC GAGAGGGAAA AAGCCCATCT GACAGACGAA 
AACAAGAAGC TGAGTGCCGA AGTGGAGAAG TTGAACGGTC AGTTCGAGCA GCTGAGCAGC 
AGTCACCGGC AGCTGGAGGA GGAGATGAGA GAACTGGCTG ATAAGAAGGA GAGTGTTGCC 
CACTGGGAGG CCCAGATCAC CGAGATCATC CAGTGGGTGA GCGATGAGAA GGATGCCCGT 
GGTTACCTTC AAGCTCTGGC CACCAAAATG ACAGAGGAGC TGGAAGGGCT CAGAAACACC 
AGTTTGGGAG CCAGAGGAAC GGACATGCCG TGGAAGATGA GGCGTTTCGC CAAGCTGGAC 
ATGTCTGCGC GTCTGGAGCT GCAGTCGGCG CTGGATACTG AGATCAGAGC CAAACAGTCC 
ATCCAAGACG AGCTTAACAA AGTCAAAGCA ACCAACATTG CCACAGAATG CAAATTGCAA 
GAATCCGAAA GTAGAAACCA GGAGCTGCTG GGAGAAATTG AGAGACTGAG GAAGGAGACA 
GAGGAGCTAA GGCTGAGGAG AGGCGTGAAG CACCAGGATT CCCAAAATTC CTTTTTGGCT 
TTCCTCAATG CGCCCACTTC TGCACTGGAT CAGTTTGATC GCTCACCCTC TTTTGGTCCC 
GGAAGCAAAA CCAGACGTCT TGACTCTGCA GATTTCACCC CTTCCAACAC TCCATCCAGA 
GATGAAGACG CAAGATCTCG CCGTATTTCA CGCTCTCGCT CACCCTCCAC CGCCAGTGAC 
ATGGAGCCTA TAGAGTTGAT TGACCACACA TCACGTAGTC TTCAGACCCC AACAGGCAGA 
TCAGGCTACG GGAGCTTGGG ACACTCATCA CCTAAGCCCA AGGCACACCA GTTTAGTGTA 
AAGTCCTTCA ATGTTCCCAC TAAATGTAAC CAGTGCACCT CTCTCATGGT GGGACTCATC 
CGTCAGGGCT GCACATGCGA AGCCTGCAGC TTCTCCTGTC ATGTCACTTG TGCTGATAAA 
GCCCCTTCTG TGTGCCCCGT CGCACCAGAC CAGACCAGGG GCAAACTGGG CATTGACACT 
CAGAGGGGCA TTGGGACTGC ATATGAGGGG CATCTTAGGG TGCCGAAGCC CACTGGGGTG 
AAGAAGGGTT GGCAGAGGGT CTGGGCAGTG GTTTGTGACT TCAAACTTTT TCTGTATGAG 
CTTGGAGAAG GGAAAGGTGC TCAGCCTGGT GTTGTAGTAA ACCAGGTCAT TGACATGAGG 
GATGAAGAGT TTTCCGCCAG TTCGGTTCTG GAGTCTGATG TCATCCATGC TAATAGGAAG 
GATATACCCT GTATTTTTAG GGTAACAGCA TCTCAGCTGT CCGCCTCCTC CAGTCAGAAG 
ACGTGTATTC TAATTCTGGC TGACAACGAG CAAGAAAGGA CGAAGTGGGT GTGCGCTCTG 
AATGAACTGC ATCGCATCCT GAGGAAAAAC AAGCTGAAGG AGCGTTTCGT CTATGTGCCT 
AAAGAAGCCT ACGACAGCAC ACTTCCTCTC ATCAAAACCA CCCAAACTGC TGCTATTATA 
GATCATGAAC GCGTTGCTTT GGGCAATGAG GAGGGCCTGT TTGTCATCCA CGTCACCAAA 
GATGAAATCA TTCGTGTGGG TGATGTCAAG AAGGTGCATC ATGTAGAACT GATGCCCACG 
GAGCACATAC TGGCCGTCAT CTCGGGCAGG AACCGACAAG TATGTCTGGT CTCCATGGCG 
GGTCTCGACG GGAGGGAGGT CGACAAGAAT AAGGTGGCAG ATACTAAAAA CTGCCAGTTG 
CTGGTGTCCG GTGTGGTTCG CAATGTCACC TGCTTCTGCC TCGCCATTAA ACGGCAGATC 
TCATGCTTCG AAATTAACAA GAGCAAGTCA CGACACTCCC ATCTCGTGGA CATACAAGCT 
CCAGGAACTG TCCAATGGAT GGCGCTGTTC AACCAGCAGT TGTGTGTTGG TTACCCATCA 
GGCTTCATGC GCTACAGTTT GTATGGAGAA ACACCTCCAG CCAGCTTACT TCATCCAGAT 
GACCCCACAC TGGCATTTAT TAAAACAGAC AACCTGGATG CCCTTTGTGC TGTGGAAATC 
TCCAATAAGG AACTGCTGCT CTGCTTCAGT AAAATAGCAG TGTATGTTGA CACACACGGC 
AGACGCTCGC GCCAGCAGGA GCTGATGTGG CCCGCGGCGC CCACCGCCTG CTGCTATAAC 
GCCCCCTATC TGTCGGTCTA CAGTGAAAAT GCAGTGGATG TGTTTGATGT GAACTCTACG 
GAATGGATTC AGACCATTCC TCTCAAAAAG GTCCGGCCAT TAAACATGGA TGGCTCTTTG 
AATCTCCTGG GCCTAGAAAC GGTCAGACTC ATCTACTTCA GAAACAAGAC CGCAGAGGGG 
GATGAACTGG TGGTGCCTGA AGTGTGTGAT AACAGCAGAA AGCAGATGGT GCGAAGCATG 
AGCAACAAGA GACGATTCTC GTTTCGTATT CCTGAGGAGG AGAGACTGCA GCAGAGGAGA 
GAAATGTTGA AAGATCCTGA GAGGAGGAAC AAGCTGATCT CAAATCCTAC AAATTTTAAC 
CATGTGGCTC ATATGGGACC TGGAGATGGC ATGCAGATAC TCAGAGACCT GCCAATGAAT 
GTACGTGCTC AGGACAACCG TGCTGGATTC AGCGGCTCCG TCAGCATCCC CTCCATTACA 
AAAAACAGAG CAGAACCAGG GCGATCCATG AGCGCAAGCA GTGGCCTAGG AATGCGGTCG 
TCCTCTCAGA ACGGCAGTGC TCTAAGGAGG GAGCTGTCCA GCGGCAGCTA CAGCTCTAAA 
CGGCAGACCA TGACTTCACC CTCCGAGAGT TCGCTGTCAT CAGGAGGAGG GATGGACGTG 
GGGGAAGCGC CGCTCTCTCA GTTTGACAGA GAGTGGCAGG CAGTATCGCC CTCGGAGAAG 
ACCCCTGATC TGAAAAGGGC TTTAAGGACC CTGCTTAGTA AGATGAAGGA CTCTGATTCG 
CCGCGGCACT CCACTGCCTC CAACAGCTCC ACCTTCAGCA GCCCGCCGAG CCCCGCCTCC 
CCACACAAGA CAAAGTCCCT CTCATTGGAG AGCACAGACC GCACCGCCTG GGACACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-89p3.3 ( XM_021468483.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324158.1 Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X3, mRNA.

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CloneID	ODa150651
Clone ID Related Accession (Same CDS sequence)	XM_021468483.1
Accession Version	XM_021468483.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase MRCK alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGTCGTCT 
CAACCTAACG ATGAGGAGCG CTTCCAGTTC CCACTTCAGG TGACTGATGT GTCCGAGGAT 
GCTAAAGATC TTGTTCGGCG GCTGATCTGC AGCAGAGAAC ACCGGCTGGG CCAGAACGGT 
ATCGAAGACT TTAAGCAGCA CCCCTTTTTC ACAGGCATCG ACTGGGACAA CATCCGCACA 
TGCGAAGCCC CCTACATCCC TGAAGTCAGC AGTCCCACTG ACACCTCCAA CTTTGATGTG 
GATGACGACT GCCTCAAAAA CTGTGAGACG TTGCCTCCAC CTTCACACAC AGCATTTTCT 
GGACACCACC TCCCATTCGT AGGCTTCACC TACACGAGTA AATGCACGCT GTCAGATCGA 
GGGTGTTTGC GGGAATCGGT CGGACCTGCA CAGGTTGATG CTCATGTTCA GCGTAGCCTG 
GAGGAGAGCC TGGCCTCTGA AGCCTATGAG AGGAGAATTC GACGTTTAGA GCAGGAGAAA 
ACCGAACTTA CCCGTAAACT ACAGGAGTCT ACGCAAACGG TGCAAGCACT GCAGTACCCT 
GATTCAGACG CCCCAGTGAA TGCTAATAAA GAGGTAGAGA TAAGGAGTTT AAAGAGTGAG 
ATCGACATCC TGAAGAAACA GATAGCAGAC TCTGGTCAGA TGGAGCAGCA GTTGGAGAAC 
GCCAGCACAG CTAGAAGAGA CCTGGAAGAC TCGTCCAAGG TCGTCAGGAA CTTAGAGAAA 
CAGCTGAAGA GTGTCACACA AGAGAAAGAT GATCTGCACA AGGAGCTGCT GGGTTTGGCA 
GAGAAGGTGA AGGCTCAGGG GAAGGAGCTA AAGGATGCTC ACAGTCAAAA AAAGATGGCC 
ATAAAGGAGT TCGCTGAGCT GAATGAACAG CTGAGCGATG TTCGCACACA AAAGCAGCGC 
CTCAGCCGAC AGTTCAAAGA CAAGGAGGAG GAGATGGAAG GAGTGTCGCA GAAACTAGAG 
GCTCTACGGC TAGAGATACG CAAAGCCGAG AGAATGCGCA AAGAGTTGGA GGCCCAGGCT 
GAGGAACATG CAGCAGAAGC TCAGAAGGAG AGAAAGGTTC GAGAAAGAAC AGAACAGTAC 
AGCAAACAAC TAGAGGAGGA GCTCGAGGGC ATGAAGCAGT TGAAGCAGAC TGGGCCTCCT 
AACGGAGCTG CAAGCTCAGA CCAGCAGCAG GAGCTGATGC GCGTTCGGGC TGAACTGGAG 
AAGAAGAGCG TACAGTACGA GGAGGAGATG TGTCGCAGAG AGACGCTCCA CAGCAGTGAG 
ATGAAGAACC TCCAAAAAGA GCTACGGGAT GCCGAGGGTC AACACCTCAC ACATCAGAAA 
GAGATACTCG TGCTTAAAGA CAAACTGGAG AAAACTCGCA GAGAAAGTCA AGTCGAGCGA 
GAAGAATTTG TGTGCGAGTA CAAGCAGAAG TACGAGAGGG AAAAAGCCCA TCTGACAGAC 
GAAAACAAGA AGCTGAGTGC CGAAGTGGAG AAGTTGAACG GTCAGTTCGA GCAGCTGAGC 
AGCAGTCACC GGCAGCTGGA GGAGGAGATG AGAGAACTGG CTGATAAGAA GGAGAGTGTT 
GCCCACTGGG AGGCCCAGAT CACCGAGATC ATCCAGTGGG TGAGCGATGA GAAGGATGCC 
CGTGGTTACC TTCAAGCTCT GGCCACCAAA ATGACAGAGG AGCTGGAAGG GCTCAGAAAC 
ACCAGTTTGG GAGCCAGAGG AACGGACATG CCGTGGAAGA TGAGGCGTTT CGCCAAGCTG 
GACATGTCTG CGCGTCTGGA GCTGCAGTCG GCGCTGGATA CTGAGATCAG AGCCAAACAG 
TCCATCCAAG ACGAGCTTAA CAAAGTCAAA GCAACCAACA TTGCCACAGA ATGCAAATTG 
CAAGAATCCG AAAGTAGAAA CCAGGAGCTG CTGGGAGAAA TTGAGAGACT GAGGAAGGAG 
ACAGAGGAGC TAAGGCTGAG GAGAGGCGTG AAGCACCAGG ATTCCCAAAA TTCCTTTTTG 
GCTTTCCTCA ATGCGCCCAC TTCTGCACTG GATCAGTTTG ATCGCTCACC CTCTTTTGGT 
CCCGGAAGCA AAACCAGACG TCTTGACTCT GCAGATTTCA CCCCTTCCAA CACTCCATCC 
AGAGATGAAG ACGCAAGATC TCGCCGTATT TCACGCTCTC GCTCACCCTC CACCGCCAGT 
GACATGGAGC CTATAGAGTT GATTGACCAC ACATCACGTA GTCTTCAGAC CCCAACAGGC 
AGATCAGGCT ACGGGAGCTT GGGACACTCA TCACCTAAGC CCAAGGCACA CCAGTTTAGT 
GTAAAGTCCT TCAATGTTCC CACTAAATGT AACCAGTGCA CCTCTCTCAT GGTGGGACTC 
ATCCGTCAGG GCTGCACATG CGAAGCCTGC AGCTTCTCCT GTCATGTCAC TTGTGCTGAT 
AAAGCCCCTT CTGTGTGCCC CGTCGCACCA GACCAGACCA GGGGCAAACT GGGCATTGAC 
ACTCAGAGGG GCATTGGGAC TGCATATGAG GGGCATCTTA GGGTGCCGAA GCCCACTGGG 
GTGAAGAAGG GTTGGCAGAG GGTCTGGGCA GTGGTTTGTG ACTTCAAACT TTTTCTGTAT 
GAGCTTGGAG AAGGGAAAGG TGCTCAGCCT GGTGTTGTAG TAAACCAGGT CATTGACATG 
AGGGATGAAG AGTTTTCCGC CAGTTCGGTT CTGGAGTCTG ATGTCATCCA TGCTAATAGG 
AAGGATATAC CCTGTATTTT TAGGGTAACA GCATCTCAGC TGTCCGCCTC CTCCAGTCAG 
AAGACGTGTA TTCTAATTCT GGCTGACAAC GAGCAAGAAA GGACGAAGTG GGTGTGCGCT 
CTGAATGAAC TGCATCGCAT CCTGAGGAAA AACAAGCTGA AGGAGCGTTT CGTCTATGTG 
CCTAAAGAAG CCTACGACAG CACACTTCCT CTCATCAAAA CCACCCAAAC TGCTGCTATT 
ATAGATCATG AACGCGTTGC TTTGGGCAAT GAGGAGGGCC TGTTTGTCAT CCACGTCACC 
AAAGATGAAA TCATTCGTGT GGGTGATGTC AAGAAGGTGC ATCATGTAGA ACTGATGCCC 
ACGGAGCACA TACTGGCCGT CATCTCGGGC AGGAACCGAC AAGTATGTCT GGTCTCCATG 
GCGGGTCTCG ACGGGAGGGA GGTCGACAAG AATAAGGTGG CAGATACTAA AAACTGCCAG 
TTGCTGGTGT CCGGTGTGGT TCGCAATGTC ACCTGCTTCT GCCTCGCCAT TAAACGGCAG 
ATCTCATGCT TCGAAATTAA CAAGAGCAAG TCACGACACT CCCATCTCGT GGACATACAA 
GCTCCAGGAA CTGTCCAATG GATGGCGCTG TTCAACCAGC AGTTGTGTGT TGGTTACCCA 
TCAGGCTTCA TGCGCTACAG TTTGTATGGA GAAACACCTC CAGCCAGCTT ACTTCATCCA 
GATGACCCCA CACTGGCATT TATTAAAACA GACAACCTGG ATGCCCTTTG TGCTGTGGAA 
ATCTCCAATA AGGAACTGCT GCTCTGCTTC AGTAAAATAG CAGTGTATGT TGACACACAC 
GGCAGACGCT CGCGCCAGCA GGAGCTGATG TGGCCCGCGG CGCCCACCGC CTGCTGCTAT 
AACGCCCCCT ATCTGTCGGT CTACAGTGAA AATGCAGTGG ATGTGTTTGA TGTGAACTCT 
ACGGAATGGA TTCAGACCAT TCCTCTCAAA AAGGTCCGGC CATTAAACAT GGATGGCTCT 
TTGAATCTCC TGGGCCTAGA AACGGTCAGA CTCATCTACT TCAGAAACAA GACCGCAGAG 
GGGGATGAAC TGGTGGTGCC TGAAGTGTGT GATAACAGCA GAAAGCAGAT GGTGCGAAGC 
ATGAGCAACA AGAGACGATT CTCGTTTCGT ATTCCTGAGG AGGAGAGACT GCAGCAGAGG 
AGAGAAATGT TGAAAGATCC TGAGAGGAGG AACAAGCTGA TCTCAAATCC TACAAATTTT 
AACCATGTGG CTCATATGGG ACCTGGAGAT GGCATGCAGA TACTCAGAGA CCTGCCAATG 
GACAACCGTG CTGGATTCAG CGGCTCCGTC AGCATCCCCT CCATTACAAA AAACAGAGCA 
GAACCAGGGC GATCCATGAG CGCAAGCAGT GGCCTAGGAA TGCGGTCGTC CTCTCAGAAC 
GGCAGTGCTC TAAGGAGGGA GCTGTCCAGC GGCAGCTACA GCTCTAAACG GCAGACCATG 
ACTTCACCCT CCGAGAGTTC GCTGTCATCA GGAGGAGGGA TGGACGTGGG GGAAGCGCCG 
CTCTCTCAGT TTGACAGAGA GTGGCAGGCA GTATCGCCCT CGGAGAAGAC CCCTGATCTG 
AAAAGGGCTT TAAGGACCCT GCTTAGTAAG ATGAAGGACT CTGATTCGCC GCGGCACTCC 
ACTGCCTCCA ACAGCTCCAC CTTCAGCAGC CCGCCGAGCC CCGCCTCCCC ACACAAGACA 
AAGTCCCTCT CATTGGAGAG CACAGACCGC ACCGCCTGGG ACACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324158.1
CDS	1641..7028
Translation	MSVEVRLKKLEKLIMDGPSQSSGQCFSMETLLDILVCLYDECSSSPIRREKNVLEFLDWAKPFTSKVKQMRLHKEDFEILKVIGRGAFGEVAVVKVKNTDKVFAMKILNKWEMLKRAETACFREERDVLVNGDCQWITTLHYAFQDENNLYLVMDYYVGGDLLTLLSKFEDRLPEDMAKFYLAEMVLAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKSSQPNDEERFQFPLQVTDVSEDAKDLVRRLICSREHRLGQNGIEDFKQHPFFTGIDWDNIRTCEAPYIPEVSSPTDTSNFDVDDDCLKNCETLPPPSHTAFSGHHLPFVGFTYTSKCTLSDRGCLRESVGPAQVDAHVQRSLEESLASEAYERRIRRLEQEKTELTRKLQESTQTVQALQYPDSDAPVNANKEVEIRSLKSEIDILKKQIADSGQMEQQLENASTARRDLEDSSKVVRNLEKQLKSVTQEKDDLHKELLGLAEKVKAQGKELKDAHSQKKMAIKEFAELNEQLSDVRTQKQRLSRQFKDKEEEMEGVSQKLEALRLEIRKAERMRKELEAQAEEHAAEAQKERKVRERTEQYSKQLEEELEGMKQLKQTGPPNGAASSDQQQELMRVRAELEKKSVQYEEEMCRRETLHSSEMKNLQKELRDAEGQHLTHQKEILVLKDKLEKTRRESQVEREEFVCEYKQKYEREKAHLTDENKKLSAEVEKLNGQFEQLSSSHRQLEEEMRELADKKESVAHWEAQITEIIQWVSDEKDARGYLQALATKMTEELEGLRNTSLGARGTDMPWKMRRFAKLDMSARLELQSALDTEIRAKQSIQDELNKVKATNIATECKLQESESRNQELLGEIERLRKETEELRLRRGVKHQDSQNSFLAFLNAPTSALDQFDRSPSFGPGSKTRRLDSADFTPSNTPSRDEDARSRRISRSRSPSTASDMEPIELIDHTSRSLQTPTGRSGYGSLGHSSPKPKAHQFSVKSFNVPTKCNQCTSLMVGLIRQGCTCEACSFSCHVTCADKAPSVCPVAPDQTRGKLGIDTQRGIGTAYEGHLRVPKPTGVKKGWQRVWAVVCDFKLFLYELGEGKGAQPGVVVNQVIDMRDEEFSASSVLESDVIHANRKDIPCIFRVTASQLSASSSQKTCILILADNEQERTKWVCALNELHRILRKNKLKERFVYVPKEAYDSTLPLIKTTQTAAIIDHERVALGNEEGLFVIHVTKDEIIRVGDVKKVHHVELMPTEHILAVISGRNRQVCLVSMAGLDGREVDKNKVADTKNCQLLVSGVVRNVTCFCLAIKRQISCFEINKSKSRHSHLVDIQAPGTVQWMALFNQQLCVGYPSGFMRYSLYGETPPASLLHPDDPTLAFIKTDNLDALCAVEISNKELLLCFSKIAVYVDTHGRRSRQQELMWPAAPTACCYNAPYLSVYSENAVDVFDVNSTEWIQTIPLKKVRPLNMDGSLNLLGLETVRLIYFRNKTAEGDELVVPEVCDNSRKQMVRSMSNKRRFSFRIPEEERLQQRREMLKDPERRNKLISNPTNFNHVAHMGPGDGMQILRDLPMDNRAGFSGSVSIPSITKNRAEPGRSMSASSGLGMRSSSQNGSALRRELSSGSYSSKRQTMTSPSESSLSSGGGMDVGEAPLSQFDREWQAVSPSEKTPDLKRALRTLLSKMKDSDSPRHSTASNSSTFSSPPSPASPHKTKSLSLESTDRTAWDT

Target ORF information:

RefSeq Version	XM_021468483.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468483.1

ORF Insert Sequence:

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGTCGTCT 
CAACCTAACG ATGAGGAGCG CTTCCAGTTC CCACTTCAGG TGACTGATGT GTCCGAGGAT 
GCTAAAGATC TTGTTCGGCG GCTGATCTGC AGCAGAGAAC ACCGGCTGGG CCAGAACGGT 
ATCGAAGACT TTAAGCAGCA CCCCTTTTTC ACAGGCATCG ACTGGGACAA CATCCGCACA 
TGCGAAGCCC CCTACATCCC TGAAGTCAGC AGTCCCACTG ACACCTCCAA CTTTGATGTG 
GATGACGACT GCCTCAAAAA CTGTGAGACG TTGCCTCCAC CTTCACACAC AGCATTTTCT 
GGACACCACC TCCCATTCGT AGGCTTCACC TACACGAGTA AATGCACGCT GTCAGATCGA 
GGGTGTTTGC GGGAATCGGT CGGACCTGCA CAGGTTGATG CTCATGTTCA GCGTAGCCTG 
GAGGAGAGCC TGGCCTCTGA AGCCTATGAG AGGAGAATTC GACGTTTAGA GCAGGAGAAA 
ACCGAACTTA CCCGTAAACT ACAGGAGTCT ACGCAAACGG TGCAAGCACT GCAGTACCCT 
GATTCAGACG CCCCAGTGAA TGCTAATAAA GAGGTAGAGA TAAGGAGTTT AAAGAGTGAG 
ATCGACATCC TGAAGAAACA GATAGCAGAC TCTGGTCAGA TGGAGCAGCA GTTGGAGAAC 
GCCAGCACAG CTAGAAGAGA CCTGGAAGAC TCGTCCAAGG TCGTCAGGAA CTTAGAGAAA 
CAGCTGAAGA GTGTCACACA AGAGAAAGAT GATCTGCACA AGGAGCTGCT GGGTTTGGCA 
GAGAAGGTGA AGGCTCAGGG GAAGGAGCTA AAGGATGCTC ACAGTCAAAA AAAGATGGCC 
ATAAAGGAGT TCGCTGAGCT GAATGAACAG CTGAGCGATG TTCGCACACA AAAGCAGCGC 
CTCAGCCGAC AGTTCAAAGA CAAGGAGGAG GAGATGGAAG GAGTGTCGCA GAAACTAGAG 
GCTCTACGGC TAGAGATACG CAAAGCCGAG AGAATGCGCA AAGAGTTGGA GGCCCAGGCT 
GAGGAACATG CAGCAGAAGC TCAGAAGGAG AGAAAGGTTC GAGAAAGAAC AGAACAGTAC 
AGCAAACAAC TAGAGGAGGA GCTCGAGGGC ATGAAGCAGT TGAAGCAGAC TGGGCCTCCT 
AACGGAGCTG CAAGCTCAGA CCAGCAGCAG GAGCTGATGC GCGTTCGGGC TGAACTGGAG 
AAGAAGAGCG TACAGTACGA GGAGGAGATG TGTCGCAGAG AGACGCTCCA CAGCAGTGAG 
ATGAAGAACC TCCAAAAAGA GCTACGGGAT GCCGAGGGTC AACACCTCAC ACATCAGAAA 
GAGATACTCG TGCTTAAAGA CAAACTGGAG AAAACTCGCA GAGAAAGTCA AGTCGAGCGA 
GAAGAATTTG TGTGCGAGTA CAAGCAGAAG TACGAGAGGG AAAAAGCCCA TCTGACAGAC 
GAAAACAAGA AGCTGAGTGC CGAAGTGGAG AAGTTGAACG GTCAGTTCGA GCAGCTGAGC 
AGCAGTCACC GGCAGCTGGA GGAGGAGATG AGAGAACTGG CTGATAAGAA GGAGAGTGTT 
GCCCACTGGG AGGCCCAGAT CACCGAGATC ATCCAGTGGG TGAGCGATGA GAAGGATGCC 
CGTGGTTACC TTCAAGCTCT GGCCACCAAA ATGACAGAGG AGCTGGAAGG GCTCAGAAAC 
ACCAGTTTGG GAGCCAGAGG AACGGACATG CCGTGGAAGA TGAGGCGTTT CGCCAAGCTG 
GACATGTCTG CGCGTCTGGA GCTGCAGTCG GCGCTGGATA CTGAGATCAG AGCCAAACAG 
TCCATCCAAG ACGAGCTTAA CAAAGTCAAA GCAACCAACA TTGCCACAGA ATGCAAATTG 
CAAGAATCCG AAAGTAGAAA CCAGGAGCTG CTGGGAGAAA TTGAGAGACT GAGGAAGGAG 
ACAGAGGAGC TAAGGCTGAG GAGAGGCGTG AAGCACCAGG ATTCCCAAAA TTCCTTTTTG 
GCTTTCCTCA ATGCGCCCAC TTCTGCACTG GATCAGTTTG ATCGCTCACC CTCTTTTGGT 
CCCGGAAGCA AAACCAGACG TCTTGACTCT GCAGATTTCA CCCCTTCCAA CACTCCATCC 
AGAGATGAAG ACGCAAGATC TCGCCGTATT TCACGCTCTC GCTCACCCTC CACCGCCAGT 
GACATGGAGC CTATAGAGTT GATTGACCAC ACATCACGTA GTCTTCAGAC CCCAACAGGC 
AGATCAGGCT ACGGGAGCTT GGGACACTCA TCACCTAAGC CCAAGGCACA CCAGTTTAGT 
GTAAAGTCCT TCAATGTTCC CACTAAATGT AACCAGTGCA CCTCTCTCAT GGTGGGACTC 
ATCCGTCAGG GCTGCACATG CGAAGCCTGC AGCTTCTCCT GTCATGTCAC TTGTGCTGAT 
AAAGCCCCTT CTGTGTGCCC CGTCGCACCA GACCAGACCA GGGGCAAACT GGGCATTGAC 
ACTCAGAGGG GCATTGGGAC TGCATATGAG GGGCATCTTA GGGTGCCGAA GCCCACTGGG 
GTGAAGAAGG GTTGGCAGAG GGTCTGGGCA GTGGTTTGTG ACTTCAAACT TTTTCTGTAT 
GAGCTTGGAG AAGGGAAAGG TGCTCAGCCT GGTGTTGTAG TAAACCAGGT CATTGACATG 
AGGGATGAAG AGTTTTCCGC CAGTTCGGTT CTGGAGTCTG ATGTCATCCA TGCTAATAGG 
AAGGATATAC CCTGTATTTT TAGGGTAACA GCATCTCAGC TGTCCGCCTC CTCCAGTCAG 
AAGACGTGTA TTCTAATTCT GGCTGACAAC GAGCAAGAAA GGACGAAGTG GGTGTGCGCT 
CTGAATGAAC TGCATCGCAT CCTGAGGAAA AACAAGCTGA AGGAGCGTTT CGTCTATGTG 
CCTAAAGAAG CCTACGACAG CACACTTCCT CTCATCAAAA CCACCCAAAC TGCTGCTATT 
ATAGATCATG AACGCGTTGC TTTGGGCAAT GAGGAGGGCC TGTTTGTCAT CCACGTCACC 
AAAGATGAAA TCATTCGTGT GGGTGATGTC AAGAAGGTGC ATCATGTAGA ACTGATGCCC 
ACGGAGCACA TACTGGCCGT CATCTCGGGC AGGAACCGAC AAGTATGTCT GGTCTCCATG 
GCGGGTCTCG ACGGGAGGGA GGTCGACAAG AATAAGGTGG CAGATACTAA AAACTGCCAG 
TTGCTGGTGT CCGGTGTGGT TCGCAATGTC ACCTGCTTCT GCCTCGCCAT TAAACGGCAG 
ATCTCATGCT TCGAAATTAA CAAGAGCAAG TCACGACACT CCCATCTCGT GGACATACAA 
GCTCCAGGAA CTGTCCAATG GATGGCGCTG TTCAACCAGC AGTTGTGTGT TGGTTACCCA 
TCAGGCTTCA TGCGCTACAG TTTGTATGGA GAAACACCTC CAGCCAGCTT ACTTCATCCA 
GATGACCCCA CACTGGCATT TATTAAAACA GACAACCTGG ATGCCCTTTG TGCTGTGGAA 
ATCTCCAATA AGGAACTGCT GCTCTGCTTC AGTAAAATAG CAGTGTATGT TGACACACAC 
GGCAGACGCT CGCGCCAGCA GGAGCTGATG TGGCCCGCGG CGCCCACCGC CTGCTGCTAT 
AACGCCCCCT ATCTGTCGGT CTACAGTGAA AATGCAGTGG ATGTGTTTGA TGTGAACTCT 
ACGGAATGGA TTCAGACCAT TCCTCTCAAA AAGGTCCGGC CATTAAACAT GGATGGCTCT 
TTGAATCTCC TGGGCCTAGA AACGGTCAGA CTCATCTACT TCAGAAACAA GACCGCAGAG 
GGGGATGAAC TGGTGGTGCC TGAAGTGTGT GATAACAGCA GAAAGCAGAT GGTGCGAAGC 
ATGAGCAACA AGAGACGATT CTCGTTTCGT ATTCCTGAGG AGGAGAGACT GCAGCAGAGG 
AGAGAAATGT TGAAAGATCC TGAGAGGAGG AACAAGCTGA TCTCAAATCC TACAAATTTT 
AACCATGTGG CTCATATGGG ACCTGGAGAT GGCATGCAGA TACTCAGAGA CCTGCCAATG 
GACAACCGTG CTGGATTCAG CGGCTCCGTC AGCATCCCCT CCATTACAAA AAACAGAGCA 
GAACCAGGGC GATCCATGAG CGCAAGCAGT GGCCTAGGAA TGCGGTCGTC CTCTCAGAAC 
GGCAGTGCTC TAAGGAGGGA GCTGTCCAGC GGCAGCTACA GCTCTAAACG GCAGACCATG 
ACTTCACCCT CCGAGAGTTC GCTGTCATCA GGAGGAGGGA TGGACGTGGG GGAAGCGCCG 
CTCTCTCAGT TTGACAGAGA GTGGCAGGCA GTATCGCCCT CGGAGAAGAC CCCTGATCTG 
AAAAGGGCTT TAAGGACCCT GCTTAGTAAG ATGAAGGACT CTGATTCGCC GCGGCACTCC 
ACTGCCTCCA ACAGCTCCAC CTTCAGCAGC CCGCCGAGCC CCGCCTCCCC ACACAAGACA 
AAGTCCCTCT CATTGGAGAG CACAGACCGC ACCGCCTGGG ACACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-89p3.3 ( XM_021468485.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324160.1 Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X6, mRNA.

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CloneID	ODa150652
Clone ID Related Accession (Same CDS sequence)	XM_021468485.1
Accession Version	XM_021468485.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase MRCK alpha isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGTCGTCT 
CAACCTAACG ATGAGGAGCG CTTCCAGTTC CCACTTCAGG TGACTGATGT GTCCGAGGAT 
GCTAAAGATC TTGTTCGGCG GCTGATCTGC AGCAGAGAAC ACCGGCTGGG CCAGAACGGT 
ATCGAAGACT TTAAGCAGCA CCCCTTTTTC ACAGGCATCG ACTGGGACAA CATCCGCACA 
TGCGAAGCCC CCTACATCCC TGAAGTCAGC AGTCCCACTG ACACCTCCAA CTTTGATGTG 
GATGACGACT GCCTCAAAAA CTGTGAGACG TTGCCTCCAC CTTCACACAC AGCATTTTCT 
GGACACCACC TCCCATTCGT AGGCTTCACC TACACGAGTA AATGCACGCT GTCAGATCGA 
GGGTGTTTGC GGGAATCGGT CGGACCTGCA CAGGTTGATG CTCATGTTCA GCGTAGCCTG 
GAGGAGAGCC TGGCCTCTGA AGCCTATGAG AGGAGAATTC GACGTTTAGA GCAGGAGAAA 
ACCGAACTTA CCCGTAAACT ACAGGAGTCT ACGCAAACGG TGCAAGCACT GCAGTACCCT 
GATTCAGACG CCCCAGTGAA TGCTAATAAA GAGGTAGAGA TAAGGAGTTT AAAGAGTGAG 
ATCGACATCC TGAAGAAACA GATAGCAGAC TCTGGTCAGA TGGAGCAGCA GTTGGAGAAC 
GCCAGCACAG CTAGAAGAGA CCTGGAAGAC TCGTCCAAGG TCGTCAGGAA CTTAGAGAAA 
CAGCTGAAGA GTGTCACACA AGAGAAAGAT GATCTGCACA AGGAGCTGCT GGGTTTGGCA 
GAGAAGGTGA AGGCTCAGGG GAAGGAGCTA AAGGATGCTC ACAGTCAAAA AAAGATGGCC 
ATAAAGGAGT TCGCTGAGCT GAATGAACAG CTGAGCGATG TTCGCACACA AAAGCAGCGC 
CTCAGCCGAC AGTTCAAAGA CAAGGAGGAG GAGATGGAAG GAGTGTCGCA GAAACTAGAG 
GCTCTACGGC TAGAGATACG CAAAGCCGAG AGAATGCGCA AAGAGTTGGA GGCCCAGGCT 
GAGGAACATG CAGCAGAAGC TCAGAAGGAG AGAAAGGTTC GAGAAAGAAC AGAACAGTAC 
AGCAAACAAC TAGAGGAGGA GCTCGAGGGC ATGAAGCAGT TGAAGCAGAC TGGGCCTCCT 
AACGGAGCTG CAAGCTCAGA CCAGCAGCAG GAGCTGATGC GCGTTCGGGC TGAACTGGAG 
AAGAAGAGCG TACAGTACGA GGAGGAGATG TGTCGCAGAG AGACGCTCCA CAGCAGTGAG 
ATGAAGAACC TCCAAAAAGA GCTACGGGAT GCCGAGGGTC AACACCTCAC ACATCAGAAA 
GAGATACTCG TGCTTAAAGA CAAACTGGAG AAAACTCGCA GAGAAAGTCA AGTCGAGCGA 
GAAGAATTTG TGTGCGAGTA CAAGCAGAAG TACGAGAGGG AAAAAGCCCA TCTGACAGAC 
GAAAACAAGA AGCTGAGTGC CGAAGTGGAG AAGTTGAACG GTCAGTTCGA GCAGCTGAGC 
AGCAGTCACC GGCAGCTGGA GGAGGAGATG AGAGAACTGG CTGATAAGAA GGAGAGTGTT 
GCCCACTGGG AGGCCCAGAT CACCGAGATC ATCCAGTGGG TGAGCGATGA GAAGGATGCC 
CGTGGTTACC TTCAAGCTCT GGCCACCAAA ATGACAGAGG AGCTGGAAGG GCTCAGAAAC 
ACCAGTTTGG GAGCCAGAGG AACGGACATG CCGTGGAAGA TGAGGCGTTT CGCCAAGCTG 
GACATGTCTG CGCGTCTGGA GCTGCAGTCG GCGCTGGATA CTGAGATCAG AGCCAAACAG 
TCCATCCAAG ACGAGCTTAA CAAAGTCAAA GCAACCAACA TTGCCACAGA ATGCAAATTG 
CAAGAATCCG AAAGTAGAAA CCAGGAGCTG CTGGGAGAAA TTGAGAGACT GAGGAAGGAG 
ACAGAGGAGC TAAGGCTGAG GAGAGGCGTG AAGCACCAGG ATTCCCAAAA TTCCTTTTTG 
GCTTTCCTCA ATGCGCCCAC TTCTGCACTG GATCAGTTTG ATCTTGACTC TGCAGATTTC 
ACCCCTTCCA ACACTCCATC CAGAGATGAA GACGCAAGAT CTCGCCGTAT TTCACGCTCT 
CGCTCACCCT CCACCGCCAG TGACATGGAG CCTATAGAGT TGATTGACCA CACATCACGT 
AGTCTTCAGA CCCCAACAGG CAGATCAGGC TACGGGAGCT TGGGACACTC ATCACCTAAG 
CCCAAGGCAC ACCAGTTTAG TGTAAAGTCC TTCAATGTTC CCACTAAATG TAACCAGTGC 
ACCTCTCTCA TGGTGGGACT CATCCGTCAG GGCTGCACAT GCGAAGCCTG CAGCTTCTCC 
TGTCATGTCA CTTGTGCTGA TAAAGCCCCT TCTGTGTGCC CCGTCGCACC AGACCAGACC 
AGGGGCAAAC TGGGCATTGA CACTCAGAGG GGCATTGGGA CTGCATATGA GGGGCATCTT 
AGGGTGCCGA AGCCCACTGG GGTGAAGAAG GGTTGGCAGA GGGTCTGGGC AGTGGTTTGT 
GACTTCAAAC TTTTTCTGTA TGAGCTTGGA GAAGGGAAAG GTGCTCAGCC TGGTGTTGTA 
GTAAACCAGG TCATTGACAT GAGGGATGAA GAGTTTTCCG CCAGTTCGGT TCTGGAGTCT 
GATGTCATCC ATGCTAATAG GAAGGATATA CCCTGTATTT TTAGGGTAAC AGCATCTCAG 
CTGTCCGCCT CCTCCAGTCA GAAGACGTGT ATTCTAATTC TGGCTGACAA CGAGCAAGAA 
AGGACGAAGT GGGTGTGCGC TCTGAATGAA CTGCATCGCA TCCTGAGGAA AAACAAGCTG 
AAGGAGCGTT TCGTCTATGT GCCTAAAGAA GCCTACGACA GCACACTTCC TCTCATCAAA 
ACCACCCAAA CTGCTGCTAT TATAGATCAT GAACGCGTTG CTTTGGGCAA TGAGGAGGGC 
CTGTTTGTCA TCCACGTCAC CAAAGATGAA ATCATTCGTG TGGGTGATGT CAAGAAGGTG 
CATCATGTAG AACTGATGCC CACGGAGCAC ATACTGGCCG TCATCTCGGG CAGGAACCGA 
CAAGTATGTC TGGTCTCCAT GGCGGGTCTC GACGGGAGGG AGGTCGACAA GAATAAGGTG 
GCAGATACTA AAAACTGCCA GTTGCTGGTG TCCGGTGTGG TTCGCAATGT CACCTGCTTC 
TGCCTCGCCA TTAAACGGCA GATCTCATGC TTCGAAATTA ACAAGAGCAA GTCACGACAC 
TCCCATCTCG TGGACATACA AGCTCCAGGA ACTGTCCAAT GGATGGCGCT GTTCAACCAG 
CAGTTGTGTG TTGGTTACCC ATCAGGCTTC ATGCGCTACA GTTTGTATGG AGAAACACCT 
CCAGCCAGCT TACTTCATCC AGATGACCCC ACACTGGCAT TTATTAAAAC AGACAACCTG 
GATGCCCTTT GTGCTGTGGA AATCTCCAAT AAGGAACTGC TGCTCTGCTT CAGTAAAATA 
GCAGTGTATG TTGACACACA CGGCAGACGC TCGCGCCAGC AGGAGCTGAT GTGGCCCGCG 
GCGCCCACCG CCTGCTGCTA TAACGCCCCC TATCTGTCGG TCTACAGTGA AAATGCAGTG 
GATGTGTTTG ATGTGAACTC TACGGAATGG ATTCAGACCA TTCCTCTCAA AAAGGTCCGG 
CCATTAAACA TGGATGGCTC TTTGAATCTC CTGGGCCTAG AAACGGTCAG ACTCATCTAC 
TTCAGAAACA AGACCGCAGA GGGGGATGAA CTGGTGGTGC CTGAAGTGTG TGATAACAGC 
AGAAAGCAGA TGGTGCGAAG CATGAGCAAC AAGAGACGAT TCTCGTTTCG TATTCCTGAG 
GAGGAGAGAC TGCAGCAGAG GAGAGAAATG TTGAAAGATC CTGAGAGGAG GAACAAGCTG 
ATCTCAAATC CTACAAATTT TAACCATGTG GCTCATATGG GACCTGGAGA TGGCATGCAG 
ATACTCAGAG ACCTGCCAAT GAATGTACGT GCTCAGGACA ACCGTGCTGG ATTCAGCGGC 
TCCGTCAGCA TCCCCTCCAT TACAAAAAAC AGAGCAGAAC CAGGGCGATC CATGAGCGCA 
AGCAGTGGCC TAGGAATGCG GTCGTCCTCT CAGAACGGCA GTGCTCTAAG GAGGGAGCTG 
TCCAGCGGCA GCTACAGCTC TAAACGGCAG ACCATGACTT CACCCTCCGA GAGTTCGCTG 
TCATCAGGAG GAGGGATGGA CGTGGGGGAA GCGCCGCTCT CTCAGTTTGA CAGAGAGTGG 
CAGGCAGTAT CGCCCTCGGA GAAGACCCCT GATCTGAAAA GGGCTTTAAG GACCCTGCTT 
AGTAAGATGA AGGACTCTGA TTCGCCGCGG CACTCCACTG CCTCCAACAG CTCCACCTTC 
AGCAGCCCGC CGAGCCCCGC CTCCCCACAC AAGACAAAGT CCCTCTCATT GGAGAGCACA 
GACCGCACCG CCTGGGACAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324160.1
CDS	1641..7004
Translation	MSVEVRLKKLEKLIMDGPSQSSGQCFSMETLLDILVCLYDECSSSPIRREKNVLEFLDWAKPFTSKVKQMRLHKEDFEILKVIGRGAFGEVAVVKVKNTDKVFAMKILNKWEMLKRAETACFREERDVLVNGDCQWITTLHYAFQDENNLYLVMDYYVGGDLLTLLSKFEDRLPEDMAKFYLAEMVLAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKSSQPNDEERFQFPLQVTDVSEDAKDLVRRLICSREHRLGQNGIEDFKQHPFFTGIDWDNIRTCEAPYIPEVSSPTDTSNFDVDDDCLKNCETLPPPSHTAFSGHHLPFVGFTYTSKCTLSDRGCLRESVGPAQVDAHVQRSLEESLASEAYERRIRRLEQEKTELTRKLQESTQTVQALQYPDSDAPVNANKEVEIRSLKSEIDILKKQIADSGQMEQQLENASTARRDLEDSSKVVRNLEKQLKSVTQEKDDLHKELLGLAEKVKAQGKELKDAHSQKKMAIKEFAELNEQLSDVRTQKQRLSRQFKDKEEEMEGVSQKLEALRLEIRKAERMRKELEAQAEEHAAEAQKERKVRERTEQYSKQLEEELEGMKQLKQTGPPNGAASSDQQQELMRVRAELEKKSVQYEEEMCRRETLHSSEMKNLQKELRDAEGQHLTHQKEILVLKDKLEKTRRESQVEREEFVCEYKQKYEREKAHLTDENKKLSAEVEKLNGQFEQLSSSHRQLEEEMRELADKKESVAHWEAQITEIIQWVSDEKDARGYLQALATKMTEELEGLRNTSLGARGTDMPWKMRRFAKLDMSARLELQSALDTEIRAKQSIQDELNKVKATNIATECKLQESESRNQELLGEIERLRKETEELRLRRGVKHQDSQNSFLAFLNAPTSALDQFDLDSADFTPSNTPSRDEDARSRRISRSRSPSTASDMEPIELIDHTSRSLQTPTGRSGYGSLGHSSPKPKAHQFSVKSFNVPTKCNQCTSLMVGLIRQGCTCEACSFSCHVTCADKAPSVCPVAPDQTRGKLGIDTQRGIGTAYEGHLRVPKPTGVKKGWQRVWAVVCDFKLFLYELGEGKGAQPGVVVNQVIDMRDEEFSASSVLESDVIHANRKDIPCIFRVTASQLSASSSQKTCILILADNEQERTKWVCALNELHRILRKNKLKERFVYVPKEAYDSTLPLIKTTQTAAIIDHERVALGNEEGLFVIHVTKDEIIRVGDVKKVHHVELMPTEHILAVISGRNRQVCLVSMAGLDGREVDKNKVADTKNCQLLVSGVVRNVTCFCLAIKRQISCFEINKSKSRHSHLVDIQAPGTVQWMALFNQQLCVGYPSGFMRYSLYGETPPASLLHPDDPTLAFIKTDNLDALCAVEISNKELLLCFSKIAVYVDTHGRRSRQQELMWPAAPTACCYNAPYLSVYSENAVDVFDVNSTEWIQTIPLKKVRPLNMDGSLNLLGLETVRLIYFRNKTAEGDELVVPEVCDNSRKQMVRSMSNKRRFSFRIPEEERLQQRREMLKDPERRNKLISNPTNFNHVAHMGPGDGMQILRDLPMNVRAQDNRAGFSGSVSIPSITKNRAEPGRSMSASSGLGMRSSSQNGSALRRELSSGSYSSKRQTMTSPSESSLSSGGGMDVGEAPLSQFDREWQAVSPSEKTPDLKRALRTLLSKMKDSDSPRHSTASNSSTFSSPPSPASPHKTKSLSLESTDRTAWDT

Target ORF information:

RefSeq Version	XM_021468485.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468485.1

ORF Insert Sequence:

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGTCGTCT 
CAACCTAACG ATGAGGAGCG CTTCCAGTTC CCACTTCAGG TGACTGATGT GTCCGAGGAT 
GCTAAAGATC TTGTTCGGCG GCTGATCTGC AGCAGAGAAC ACCGGCTGGG CCAGAACGGT 
ATCGAAGACT TTAAGCAGCA CCCCTTTTTC ACAGGCATCG ACTGGGACAA CATCCGCACA 
TGCGAAGCCC CCTACATCCC TGAAGTCAGC AGTCCCACTG ACACCTCCAA CTTTGATGTG 
GATGACGACT GCCTCAAAAA CTGTGAGACG TTGCCTCCAC CTTCACACAC AGCATTTTCT 
GGACACCACC TCCCATTCGT AGGCTTCACC TACACGAGTA AATGCACGCT GTCAGATCGA 
GGGTGTTTGC GGGAATCGGT CGGACCTGCA CAGGTTGATG CTCATGTTCA GCGTAGCCTG 
GAGGAGAGCC TGGCCTCTGA AGCCTATGAG AGGAGAATTC GACGTTTAGA GCAGGAGAAA 
ACCGAACTTA CCCGTAAACT ACAGGAGTCT ACGCAAACGG TGCAAGCACT GCAGTACCCT 
GATTCAGACG CCCCAGTGAA TGCTAATAAA GAGGTAGAGA TAAGGAGTTT AAAGAGTGAG 
ATCGACATCC TGAAGAAACA GATAGCAGAC TCTGGTCAGA TGGAGCAGCA GTTGGAGAAC 
GCCAGCACAG CTAGAAGAGA CCTGGAAGAC TCGTCCAAGG TCGTCAGGAA CTTAGAGAAA 
CAGCTGAAGA GTGTCACACA AGAGAAAGAT GATCTGCACA AGGAGCTGCT GGGTTTGGCA 
GAGAAGGTGA AGGCTCAGGG GAAGGAGCTA AAGGATGCTC ACAGTCAAAA AAAGATGGCC 
ATAAAGGAGT TCGCTGAGCT GAATGAACAG CTGAGCGATG TTCGCACACA AAAGCAGCGC 
CTCAGCCGAC AGTTCAAAGA CAAGGAGGAG GAGATGGAAG GAGTGTCGCA GAAACTAGAG 
GCTCTACGGC TAGAGATACG CAAAGCCGAG AGAATGCGCA AAGAGTTGGA GGCCCAGGCT 
GAGGAACATG CAGCAGAAGC TCAGAAGGAG AGAAAGGTTC GAGAAAGAAC AGAACAGTAC 
AGCAAACAAC TAGAGGAGGA GCTCGAGGGC ATGAAGCAGT TGAAGCAGAC TGGGCCTCCT 
AACGGAGCTG CAAGCTCAGA CCAGCAGCAG GAGCTGATGC GCGTTCGGGC TGAACTGGAG 
AAGAAGAGCG TACAGTACGA GGAGGAGATG TGTCGCAGAG AGACGCTCCA CAGCAGTGAG 
ATGAAGAACC TCCAAAAAGA GCTACGGGAT GCCGAGGGTC AACACCTCAC ACATCAGAAA 
GAGATACTCG TGCTTAAAGA CAAACTGGAG AAAACTCGCA GAGAAAGTCA AGTCGAGCGA 
GAAGAATTTG TGTGCGAGTA CAAGCAGAAG TACGAGAGGG AAAAAGCCCA TCTGACAGAC 
GAAAACAAGA AGCTGAGTGC CGAAGTGGAG AAGTTGAACG GTCAGTTCGA GCAGCTGAGC 
AGCAGTCACC GGCAGCTGGA GGAGGAGATG AGAGAACTGG CTGATAAGAA GGAGAGTGTT 
GCCCACTGGG AGGCCCAGAT CACCGAGATC ATCCAGTGGG TGAGCGATGA GAAGGATGCC 
CGTGGTTACC TTCAAGCTCT GGCCACCAAA ATGACAGAGG AGCTGGAAGG GCTCAGAAAC 
ACCAGTTTGG GAGCCAGAGG AACGGACATG CCGTGGAAGA TGAGGCGTTT CGCCAAGCTG 
GACATGTCTG CGCGTCTGGA GCTGCAGTCG GCGCTGGATA CTGAGATCAG AGCCAAACAG 
TCCATCCAAG ACGAGCTTAA CAAAGTCAAA GCAACCAACA TTGCCACAGA ATGCAAATTG 
CAAGAATCCG AAAGTAGAAA CCAGGAGCTG CTGGGAGAAA TTGAGAGACT GAGGAAGGAG 
ACAGAGGAGC TAAGGCTGAG GAGAGGCGTG AAGCACCAGG ATTCCCAAAA TTCCTTTTTG 
GCTTTCCTCA ATGCGCCCAC TTCTGCACTG GATCAGTTTG ATCTTGACTC TGCAGATTTC 
ACCCCTTCCA ACACTCCATC CAGAGATGAA GACGCAAGAT CTCGCCGTAT TTCACGCTCT 
CGCTCACCCT CCACCGCCAG TGACATGGAG CCTATAGAGT TGATTGACCA CACATCACGT 
AGTCTTCAGA CCCCAACAGG CAGATCAGGC TACGGGAGCT TGGGACACTC ATCACCTAAG 
CCCAAGGCAC ACCAGTTTAG TGTAAAGTCC TTCAATGTTC CCACTAAATG TAACCAGTGC 
ACCTCTCTCA TGGTGGGACT CATCCGTCAG GGCTGCACAT GCGAAGCCTG CAGCTTCTCC 
TGTCATGTCA CTTGTGCTGA TAAAGCCCCT TCTGTGTGCC CCGTCGCACC AGACCAGACC 
AGGGGCAAAC TGGGCATTGA CACTCAGAGG GGCATTGGGA CTGCATATGA GGGGCATCTT 
AGGGTGCCGA AGCCCACTGG GGTGAAGAAG GGTTGGCAGA GGGTCTGGGC AGTGGTTTGT 
GACTTCAAAC TTTTTCTGTA TGAGCTTGGA GAAGGGAAAG GTGCTCAGCC TGGTGTTGTA 
GTAAACCAGG TCATTGACAT GAGGGATGAA GAGTTTTCCG CCAGTTCGGT TCTGGAGTCT 
GATGTCATCC ATGCTAATAG GAAGGATATA CCCTGTATTT TTAGGGTAAC AGCATCTCAG 
CTGTCCGCCT CCTCCAGTCA GAAGACGTGT ATTCTAATTC TGGCTGACAA CGAGCAAGAA 
AGGACGAAGT GGGTGTGCGC TCTGAATGAA CTGCATCGCA TCCTGAGGAA AAACAAGCTG 
AAGGAGCGTT TCGTCTATGT GCCTAAAGAA GCCTACGACA GCACACTTCC TCTCATCAAA 
ACCACCCAAA CTGCTGCTAT TATAGATCAT GAACGCGTTG CTTTGGGCAA TGAGGAGGGC 
CTGTTTGTCA TCCACGTCAC CAAAGATGAA ATCATTCGTG TGGGTGATGT CAAGAAGGTG 
CATCATGTAG AACTGATGCC CACGGAGCAC ATACTGGCCG TCATCTCGGG CAGGAACCGA 
CAAGTATGTC TGGTCTCCAT GGCGGGTCTC GACGGGAGGG AGGTCGACAA GAATAAGGTG 
GCAGATACTA AAAACTGCCA GTTGCTGGTG TCCGGTGTGG TTCGCAATGT CACCTGCTTC 
TGCCTCGCCA TTAAACGGCA GATCTCATGC TTCGAAATTA ACAAGAGCAA GTCACGACAC 
TCCCATCTCG TGGACATACA AGCTCCAGGA ACTGTCCAAT GGATGGCGCT GTTCAACCAG 
CAGTTGTGTG TTGGTTACCC ATCAGGCTTC ATGCGCTACA GTTTGTATGG AGAAACACCT 
CCAGCCAGCT TACTTCATCC AGATGACCCC ACACTGGCAT TTATTAAAAC AGACAACCTG 
GATGCCCTTT GTGCTGTGGA AATCTCCAAT AAGGAACTGC TGCTCTGCTT CAGTAAAATA 
GCAGTGTATG TTGACACACA CGGCAGACGC TCGCGCCAGC AGGAGCTGAT GTGGCCCGCG 
GCGCCCACCG CCTGCTGCTA TAACGCCCCC TATCTGTCGG TCTACAGTGA AAATGCAGTG 
GATGTGTTTG ATGTGAACTC TACGGAATGG ATTCAGACCA TTCCTCTCAA AAAGGTCCGG 
CCATTAAACA TGGATGGCTC TTTGAATCTC CTGGGCCTAG AAACGGTCAG ACTCATCTAC 
TTCAGAAACA AGACCGCAGA GGGGGATGAA CTGGTGGTGC CTGAAGTGTG TGATAACAGC 
AGAAAGCAGA TGGTGCGAAG CATGAGCAAC AAGAGACGAT TCTCGTTTCG TATTCCTGAG 
GAGGAGAGAC TGCAGCAGAG GAGAGAAATG TTGAAAGATC CTGAGAGGAG GAACAAGCTG 
ATCTCAAATC CTACAAATTT TAACCATGTG GCTCATATGG GACCTGGAGA TGGCATGCAG 
ATACTCAGAG ACCTGCCAAT GAATGTACGT GCTCAGGACA ACCGTGCTGG ATTCAGCGGC 
TCCGTCAGCA TCCCCTCCAT TACAAAAAAC AGAGCAGAAC CAGGGCGATC CATGAGCGCA 
AGCAGTGGCC TAGGAATGCG GTCGTCCTCT CAGAACGGCA GTGCTCTAAG GAGGGAGCTG 
TCCAGCGGCA GCTACAGCTC TAAACGGCAG ACCATGACTT CACCCTCCGA GAGTTCGCTG 
TCATCAGGAG GAGGGATGGA CGTGGGGGAA GCGCCGCTCT CTCAGTTTGA CAGAGAGTGG 
CAGGCAGTAT CGCCCTCGGA GAAGACCCCT GATCTGAAAA GGGCTTTAAG GACCCTGCTT 
AGTAAGATGA AGGACTCTGA TTCGCCGCGG CACTCCACTG CCTCCAACAG CTCCACCTTC 
AGCAGCCCGC CGAGCCCCGC CTCCCCACAC AAGACAAAGT CCCTCTCATT GGAGAGCACA 
GACCGCACCG CCTGGGACAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-89p3.3 ( XM_021468486.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324161.1 Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X7, mRNA.

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CloneID	ODa150653
Clone ID Related Accession (Same CDS sequence)	XM_021468486.1
Accession Version	XM_021468486.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase MRCK alpha isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGTCGTCT 
CAACCTAACG ATGAGGAGCG CTTCCAGTTC CCACTTCAGG TGACTGATGT GTCCGAGGAT 
GCTAAAGATC TTGTTCGGCG GCTGATCTGC AGCAGAGAAC ACCGGCTGGG CCAGAACGGT 
ATCGAAGACT TTAAGCAGCA CCCCTTTTTC ACAGGCATCG ACTGGGACAA CATCCGCACA 
TGCGAAGCCC CCTACATCCC TGAAGTCAGC AGTCCCACTG ACACCTCCAA CTTTGATGTG 
GATGACGACT GCCTCAAAAA CTGTGAGACG TTGCCTCCAC CTTCACACAC AGCATTTTCT 
GGACACCACC TCCCATTCGT AGGCTTCACC TACACGAGTA AATGCACGCT GTCAGATCGA 
GGGTGTTTGC GGGAATCGGT CGGACCTGCA CAGGTTGATG CTCATGTTCA GCGTAGCCTG 
GAGGAGAGCC TGGCCTCTGA AGCCTATGAG AGGAGAATTC GACGTTTAGA GCAGGAGAAA 
ACCGAACTTA CCCGTAAACT ACAGGAGTCT ACGCAAACGG TGCAAGCACT GCAGTACCCT 
GATTCAGACG CCCCAGTGAA TGCTAATAAA GAGGTAGAGA TAAGGAGTTT AAAGAGTGAG 
ATCGACATCC TGAAGAAACA GATAGCAGAC TCTGGTCAGA TGGAGCAGCA GTTGGAGAAC 
GCCAGCACAG CTAGAAGAGA CCTGGAAGAC TCGTCCAAGG TCGTCAGGAA CTTAGAGAAA 
CAGCTGAAGA GTGTCACACA AGAGAAAGAT GATCTGCACA AGGAGCTGCT GGGTTTGGCA 
GAGAAGGTGA AGGCTCAGGG GAAGGAGCTA AAGGATGCTC ACAGTCAAAA AAAGATGGCC 
ATAAAGGAGT TCGCTGAGCT GAATGAACAG CTGAGCGATG TTCGCACACA AAAGCAGCGC 
CTCAGCCGAC AGTTCAAAGA CAAGGAGGAG GAGATGGAAG GAGTGTCGCA GAAACTAGAG 
GCTCTACGGC TAGAGATACG CAAAGCCGAG AGAATGCGCA AAGAGTTGGA GGCCCAGGCT 
GAGGAACATG CAGCAGAAGC TCAGAAGGAG AGAAAGGTTC GAGAAAGAAC AGAACAGTAC 
AGCAAACAAC TAGAGGAGGA GCTCGAGGGC ATGAAGCAGT TGAAGCAGAC TGGGCCTCCT 
AACGGAGCTG CAAGCTCAGA CCAGCAGCAG GAGCTGATGC GCGTTCGGGC TGAACTGGAG 
AAGAAGAGCG TACAGTACGA GGAGGAGATG TGTCGCAGAG AGACGCTCCA CAGCAGTGAG 
ATGAAGAACC TCCAAAAAGA GCTACGGGAT GCCGAGGGTC AACACCTCAC ACATCAGAAA 
GAGATACTCG TGCTTAAAGA CAAACTGGAG AAAACTCGCA GAGAAAGTCA AGTCGAGCGA 
GAAGAATTTG TGTGCGAGTA CAAGCAGAAG TACGAGAGGG AAAAAGCCCA TCTGACAGAC 
GAAAACAAGA AGCTGAGTGC CGAAGTGGAG AAGTTGAACG GTCAGTTCGA GCAGCTGAGC 
AGCAGTCACC GGCAGCTGGA GGAGGAGATG AGAGAACTGG CTGATAAGAA GGAGAGTGTT 
GCCCACTGGG AGGCCCAGAT CACCGAGATC ATCCAGTGGG TGAGCGATGA GAAGGATGCC 
CGTGGTTACC TTCAAGCTCT GGCCACCAAA ATGACAGAGG AGCTGGAAGG GCTCAGAAAC 
ACCAGTTTGG GAGCCAGAGG AACGGACATG CCGTGGAAGA TGAGGCGTTT CGCCAAGCTG 
GACATGTCTG CGCGTCTGGA GCTGCAGTCG GCGCTGGATA CTGAGATCAG AGCCAAACAG 
TCCATCCAAG ACGAGCTTAA CAAAGTCAAA GCAACCAACA TTGCCACAGA ATGCAAATTG 
CAAGAATCCG AAAGTAGAAA CCAGGAGCTG CTGGGAGAAA TTGAGAGACT GAGGAAGGAG 
ACAGAGGAGC TAAGGCTGAG GAGAGGCGTG AAGCACCAGG ATTCCCAAAA TTCCTTTTTG 
GCTTTCCTCA ATGCGCCCAC TTCTGCACTG GATCAGTTTG ATCGCTCACC CTCTTTTGGT 
CCCGGAAGCA AAACCAGACG TCTTGACTCT GCAGATTTCA CCCCTTCCAA CACTCCATCC 
AGAGATGAAG ACGCAAGATC TCGCCGTATT TCACGCTCTC GCTCACCCTC CACCGCCAGT 
GACATGGAGC CTATAGAGTT GATTGACCAC ACATCACGTA GTCTTCAGAC CCCAACAGGC 
AGATCAGGCT ACGGGAGCTT GGGACACTCA TCACCTAAGC CCAAGGCACA CCAGTTTAGT 
GTAAAGTCCT TCAATGTTCC CACTAAATGT AACCAGTGCA CCTCTCTCAT GGTGGGACTC 
ATCCGTCAGG GCTGCACATG CGAAGCCTGC AGCTTCTCCT GTCATGTCAC TTGTGCTGAT 
AAAGCCCCTT CTGTGTGCCC CGTCGCACCA GACCAGACCA GGGGCAAACT GGGCATTGAC 
ACTCAGAGGG GCATTGGGAC TGCATATGAG GGGCATCTTA GGGTGCCGAA GCCCACTGGG 
GTGAAGAAGG GTTGGCAGAG GGTCTGGGCA GTGGTTTGTG ACTTCAAACT TTTTCTGTAT 
GAGCTTGGAG AAGGGAAAGG TGCTCAGCCT GGTGTTGTAG TAAACCAGGT CATTGACATG 
AGGGATGAAG AGTTTTCCGC CAGTTCGGTT CTGGAGTCTG ATGTCATCCA TGCTAATAGG 
AAGGATATAC CCTGTATTTT TAGGGTAACA GCATCTCAGC TGTCCGCCTC CTCCAGTCAG 
AAGACGTGTA TTCTAATTCT GGCTGACAAC GAGCAAGAAA GGACGAAGTG GGTGTGCGCT 
CTGAATGAAC TGCATCGCAT CCTGAGGAAA AACAAGCTGA AGGAGCGTTT CGTCTATGTG 
CCTAAAGAAG CCTACGACAG CACACTTCCT CTCATCAAAA CCACCCAAAC TGCTGCTATT 
ATAGATCATG AACGCGTTGC TTTGGGCAAT GAGGAGGGCC TGTTTGTCAT CCACGTCACC 
AAAGATGAAA TCATTCGTGT GGGTGATGTC AAGAAGGTGC ATCATGTAGA ACTGATGCCC 
ACGGAGCACA TACTGGCCGT CATCTCGGGC AGGAACCGAC AAGTATGTCT GGTCTCCATG 
GCGGGTCTCG ACGGGAGGGA GGTCGACAAG AATAAGGTGG CAGATACTAA AAACTGCCAG 
TTGCTGGTGT CCGGTGTGGT TCGCAATGTC ACCTGCTTCT GCCTCGCCAT TAAACGGCAG 
ATCTCATGCT TCGAAATTAA CAAGAGCAAG TCACGACACT CCCATCTCGT GGACATACAA 
GCTCCAGGAA CTGTCCAATG GATGGCGCTG TTCAACCAGC AGTTGTGTGT TGGTTACCCA 
TCAGGCTTCA TGCGCTACAG TTTGTATGGA GAAACACCTC CAGCCAGCTT ACTTCATCCA 
GATGACCCCA CACTGGCATT TATTAAAACA GACAACCTGG ATGCCCTTTG TGCTGTGGAA 
ATCTCCAATA AGGAACTGCT GCTCTGCTTC AGTAAAATAG CAGTGTATGT TGACACACAC 
GGCAGACGCT CGCGCCAGCA GGAGCTGATG TGGCCCGCGG CGCCCACCGC CTGCTGCTAT 
AACGCCCCCT ATCTGTCGGT CTACAGTGAA AATGCAGTGG ATGTGTTTGA TGTGAACTCT 
ACGGAATGGA TTCAGACCAT TCCTCTCAAA AAGGTCCGGC CATTAAACAT GGATGGCTCT 
TTGAATCTCC TGGGCCTAGA AACGGTCAGA CTCATCTACT TCAGAAACAA GACCGCAGAG 
GGGGATGAAC TGGTGGTGCC TGAAGTGTGT GATAACAGCA GAAAGCAGAT GGTGCGAAGC 
ATGAGCAACA AGAGACGATT CTCGTTTCGT ATTCCTGAGG AGGAGAGACT GCAGCAGAGG 
AGAGAAATGT TGAAAGATCC TGAGAGGAGG AACAAGCTGA TCTCAAATCC TACAAATTTT 
AACCATGTGG CTCATATGGG ACCTGGAGAT GGCATGCAGA TACTCAGAGA CCTGCCAATG 
AATGTACGTG CTCAGGACAA CCGTGCTGGA TTCAGCGGCT CCGTCAGCAT CCCCTCCATT 
ACAAAAAACA GAGCAGAACC AGGGCGATCC ATGAGCGCAA GCAGTGGCCT AGGAATGCGG 
TCGTCCTCTC AGAACGGCAG TGCTCTAAGG AGGGAGCTGT CCAGCGGCAG CTACAGCTCT 
AAACGGCAGA CCATGACTTC ACCCTCCGAG AGTTCGCTGT CATCAGGAGG AGGGATGGAC 
GTGGGGGAAG CGCCGCTCTC TCAGTTTGAC AGAGAGGACT CTGATTCGCC GCGGCACTCC 
ACTGCCTCCA ACAGCTCCAC CTTCAGCAGC CCGCCGAGCC CCGCCTCCCC ACACAAGACA 
AAGTCCCTCT CATTGGAGAG CACAGACCGC ACCGCCTGGG ACACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324161.1
CDS	1641..6968
Translation	MSVEVRLKKLEKLIMDGPSQSSGQCFSMETLLDILVCLYDECSSSPIRREKNVLEFLDWAKPFTSKVKQMRLHKEDFEILKVIGRGAFGEVAVVKVKNTDKVFAMKILNKWEMLKRAETACFREERDVLVNGDCQWITTLHYAFQDENNLYLVMDYYVGGDLLTLLSKFEDRLPEDMAKFYLAEMVLAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKSSQPNDEERFQFPLQVTDVSEDAKDLVRRLICSREHRLGQNGIEDFKQHPFFTGIDWDNIRTCEAPYIPEVSSPTDTSNFDVDDDCLKNCETLPPPSHTAFSGHHLPFVGFTYTSKCTLSDRGCLRESVGPAQVDAHVQRSLEESLASEAYERRIRRLEQEKTELTRKLQESTQTVQALQYPDSDAPVNANKEVEIRSLKSEIDILKKQIADSGQMEQQLENASTARRDLEDSSKVVRNLEKQLKSVTQEKDDLHKELLGLAEKVKAQGKELKDAHSQKKMAIKEFAELNEQLSDVRTQKQRLSRQFKDKEEEMEGVSQKLEALRLEIRKAERMRKELEAQAEEHAAEAQKERKVRERTEQYSKQLEEELEGMKQLKQTGPPNGAASSDQQQELMRVRAELEKKSVQYEEEMCRRETLHSSEMKNLQKELRDAEGQHLTHQKEILVLKDKLEKTRRESQVEREEFVCEYKQKYEREKAHLTDENKKLSAEVEKLNGQFEQLSSSHRQLEEEMRELADKKESVAHWEAQITEIIQWVSDEKDARGYLQALATKMTEELEGLRNTSLGARGTDMPWKMRRFAKLDMSARLELQSALDTEIRAKQSIQDELNKVKATNIATECKLQESESRNQELLGEIERLRKETEELRLRRGVKHQDSQNSFLAFLNAPTSALDQFDRSPSFGPGSKTRRLDSADFTPSNTPSRDEDARSRRISRSRSPSTASDMEPIELIDHTSRSLQTPTGRSGYGSLGHSSPKPKAHQFSVKSFNVPTKCNQCTSLMVGLIRQGCTCEACSFSCHVTCADKAPSVCPVAPDQTRGKLGIDTQRGIGTAYEGHLRVPKPTGVKKGWQRVWAVVCDFKLFLYELGEGKGAQPGVVVNQVIDMRDEEFSASSVLESDVIHANRKDIPCIFRVTASQLSASSSQKTCILILADNEQERTKWVCALNELHRILRKNKLKERFVYVPKEAYDSTLPLIKTTQTAAIIDHERVALGNEEGLFVIHVTKDEIIRVGDVKKVHHVELMPTEHILAVISGRNRQVCLVSMAGLDGREVDKNKVADTKNCQLLVSGVVRNVTCFCLAIKRQISCFEINKSKSRHSHLVDIQAPGTVQWMALFNQQLCVGYPSGFMRYSLYGETPPASLLHPDDPTLAFIKTDNLDALCAVEISNKELLLCFSKIAVYVDTHGRRSRQQELMWPAAPTACCYNAPYLSVYSENAVDVFDVNSTEWIQTIPLKKVRPLNMDGSLNLLGLETVRLIYFRNKTAEGDELVVPEVCDNSRKQMVRSMSNKRRFSFRIPEEERLQQRREMLKDPERRNKLISNPTNFNHVAHMGPGDGMQILRDLPMNVRAQDNRAGFSGSVSIPSITKNRAEPGRSMSASSGLGMRSSSQNGSALRRELSSGSYSSKRQTMTSPSESSLSSGGGMDVGEAPLSQFDREDSDSPRHSTASNSSTFSSPPSPASPHKTKSLSLESTDRTAWDT

Target ORF information:

RefSeq Version	XM_021468486.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468486.1

ORF Insert Sequence:

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGTCGTCT 
CAACCTAACG ATGAGGAGCG CTTCCAGTTC CCACTTCAGG TGACTGATGT GTCCGAGGAT 
GCTAAAGATC TTGTTCGGCG GCTGATCTGC AGCAGAGAAC ACCGGCTGGG CCAGAACGGT 
ATCGAAGACT TTAAGCAGCA CCCCTTTTTC ACAGGCATCG ACTGGGACAA CATCCGCACA 
TGCGAAGCCC CCTACATCCC TGAAGTCAGC AGTCCCACTG ACACCTCCAA CTTTGATGTG 
GATGACGACT GCCTCAAAAA CTGTGAGACG TTGCCTCCAC CTTCACACAC AGCATTTTCT 
GGACACCACC TCCCATTCGT AGGCTTCACC TACACGAGTA AATGCACGCT GTCAGATCGA 
GGGTGTTTGC GGGAATCGGT CGGACCTGCA CAGGTTGATG CTCATGTTCA GCGTAGCCTG 
GAGGAGAGCC TGGCCTCTGA AGCCTATGAG AGGAGAATTC GACGTTTAGA GCAGGAGAAA 
ACCGAACTTA CCCGTAAACT ACAGGAGTCT ACGCAAACGG TGCAAGCACT GCAGTACCCT 
GATTCAGACG CCCCAGTGAA TGCTAATAAA GAGGTAGAGA TAAGGAGTTT AAAGAGTGAG 
ATCGACATCC TGAAGAAACA GATAGCAGAC TCTGGTCAGA TGGAGCAGCA GTTGGAGAAC 
GCCAGCACAG CTAGAAGAGA CCTGGAAGAC TCGTCCAAGG TCGTCAGGAA CTTAGAGAAA 
CAGCTGAAGA GTGTCACACA AGAGAAAGAT GATCTGCACA AGGAGCTGCT GGGTTTGGCA 
GAGAAGGTGA AGGCTCAGGG GAAGGAGCTA AAGGATGCTC ACAGTCAAAA AAAGATGGCC 
ATAAAGGAGT TCGCTGAGCT GAATGAACAG CTGAGCGATG TTCGCACACA AAAGCAGCGC 
CTCAGCCGAC AGTTCAAAGA CAAGGAGGAG GAGATGGAAG GAGTGTCGCA GAAACTAGAG 
GCTCTACGGC TAGAGATACG CAAAGCCGAG AGAATGCGCA AAGAGTTGGA GGCCCAGGCT 
GAGGAACATG CAGCAGAAGC TCAGAAGGAG AGAAAGGTTC GAGAAAGAAC AGAACAGTAC 
AGCAAACAAC TAGAGGAGGA GCTCGAGGGC ATGAAGCAGT TGAAGCAGAC TGGGCCTCCT 
AACGGAGCTG CAAGCTCAGA CCAGCAGCAG GAGCTGATGC GCGTTCGGGC TGAACTGGAG 
AAGAAGAGCG TACAGTACGA GGAGGAGATG TGTCGCAGAG AGACGCTCCA CAGCAGTGAG 
ATGAAGAACC TCCAAAAAGA GCTACGGGAT GCCGAGGGTC AACACCTCAC ACATCAGAAA 
GAGATACTCG TGCTTAAAGA CAAACTGGAG AAAACTCGCA GAGAAAGTCA AGTCGAGCGA 
GAAGAATTTG TGTGCGAGTA CAAGCAGAAG TACGAGAGGG AAAAAGCCCA TCTGACAGAC 
GAAAACAAGA AGCTGAGTGC CGAAGTGGAG AAGTTGAACG GTCAGTTCGA GCAGCTGAGC 
AGCAGTCACC GGCAGCTGGA GGAGGAGATG AGAGAACTGG CTGATAAGAA GGAGAGTGTT 
GCCCACTGGG AGGCCCAGAT CACCGAGATC ATCCAGTGGG TGAGCGATGA GAAGGATGCC 
CGTGGTTACC TTCAAGCTCT GGCCACCAAA ATGACAGAGG AGCTGGAAGG GCTCAGAAAC 
ACCAGTTTGG GAGCCAGAGG AACGGACATG CCGTGGAAGA TGAGGCGTTT CGCCAAGCTG 
GACATGTCTG CGCGTCTGGA GCTGCAGTCG GCGCTGGATA CTGAGATCAG AGCCAAACAG 
TCCATCCAAG ACGAGCTTAA CAAAGTCAAA GCAACCAACA TTGCCACAGA ATGCAAATTG 
CAAGAATCCG AAAGTAGAAA CCAGGAGCTG CTGGGAGAAA TTGAGAGACT GAGGAAGGAG 
ACAGAGGAGC TAAGGCTGAG GAGAGGCGTG AAGCACCAGG ATTCCCAAAA TTCCTTTTTG 
GCTTTCCTCA ATGCGCCCAC TTCTGCACTG GATCAGTTTG ATCGCTCACC CTCTTTTGGT 
CCCGGAAGCA AAACCAGACG TCTTGACTCT GCAGATTTCA CCCCTTCCAA CACTCCATCC 
AGAGATGAAG ACGCAAGATC TCGCCGTATT TCACGCTCTC GCTCACCCTC CACCGCCAGT 
GACATGGAGC CTATAGAGTT GATTGACCAC ACATCACGTA GTCTTCAGAC CCCAACAGGC 
AGATCAGGCT ACGGGAGCTT GGGACACTCA TCACCTAAGC CCAAGGCACA CCAGTTTAGT 
GTAAAGTCCT TCAATGTTCC CACTAAATGT AACCAGTGCA CCTCTCTCAT GGTGGGACTC 
ATCCGTCAGG GCTGCACATG CGAAGCCTGC AGCTTCTCCT GTCATGTCAC TTGTGCTGAT 
AAAGCCCCTT CTGTGTGCCC CGTCGCACCA GACCAGACCA GGGGCAAACT GGGCATTGAC 
ACTCAGAGGG GCATTGGGAC TGCATATGAG GGGCATCTTA GGGTGCCGAA GCCCACTGGG 
GTGAAGAAGG GTTGGCAGAG GGTCTGGGCA GTGGTTTGTG ACTTCAAACT TTTTCTGTAT 
GAGCTTGGAG AAGGGAAAGG TGCTCAGCCT GGTGTTGTAG TAAACCAGGT CATTGACATG 
AGGGATGAAG AGTTTTCCGC CAGTTCGGTT CTGGAGTCTG ATGTCATCCA TGCTAATAGG 
AAGGATATAC CCTGTATTTT TAGGGTAACA GCATCTCAGC TGTCCGCCTC CTCCAGTCAG 
AAGACGTGTA TTCTAATTCT GGCTGACAAC GAGCAAGAAA GGACGAAGTG GGTGTGCGCT 
CTGAATGAAC TGCATCGCAT CCTGAGGAAA AACAAGCTGA AGGAGCGTTT CGTCTATGTG 
CCTAAAGAAG CCTACGACAG CACACTTCCT CTCATCAAAA CCACCCAAAC TGCTGCTATT 
ATAGATCATG AACGCGTTGC TTTGGGCAAT GAGGAGGGCC TGTTTGTCAT CCACGTCACC 
AAAGATGAAA TCATTCGTGT GGGTGATGTC AAGAAGGTGC ATCATGTAGA ACTGATGCCC 
ACGGAGCACA TACTGGCCGT CATCTCGGGC AGGAACCGAC AAGTATGTCT GGTCTCCATG 
GCGGGTCTCG ACGGGAGGGA GGTCGACAAG AATAAGGTGG CAGATACTAA AAACTGCCAG 
TTGCTGGTGT CCGGTGTGGT TCGCAATGTC ACCTGCTTCT GCCTCGCCAT TAAACGGCAG 
ATCTCATGCT TCGAAATTAA CAAGAGCAAG TCACGACACT CCCATCTCGT GGACATACAA 
GCTCCAGGAA CTGTCCAATG GATGGCGCTG TTCAACCAGC AGTTGTGTGT TGGTTACCCA 
TCAGGCTTCA TGCGCTACAG TTTGTATGGA GAAACACCTC CAGCCAGCTT ACTTCATCCA 
GATGACCCCA CACTGGCATT TATTAAAACA GACAACCTGG ATGCCCTTTG TGCTGTGGAA 
ATCTCCAATA AGGAACTGCT GCTCTGCTTC AGTAAAATAG CAGTGTATGT TGACACACAC 
GGCAGACGCT CGCGCCAGCA GGAGCTGATG TGGCCCGCGG CGCCCACCGC CTGCTGCTAT 
AACGCCCCCT ATCTGTCGGT CTACAGTGAA AATGCAGTGG ATGTGTTTGA TGTGAACTCT 
ACGGAATGGA TTCAGACCAT TCCTCTCAAA AAGGTCCGGC CATTAAACAT GGATGGCTCT 
TTGAATCTCC TGGGCCTAGA AACGGTCAGA CTCATCTACT TCAGAAACAA GACCGCAGAG 
GGGGATGAAC TGGTGGTGCC TGAAGTGTGT GATAACAGCA GAAAGCAGAT GGTGCGAAGC 
ATGAGCAACA AGAGACGATT CTCGTTTCGT ATTCCTGAGG AGGAGAGACT GCAGCAGAGG 
AGAGAAATGT TGAAAGATCC TGAGAGGAGG AACAAGCTGA TCTCAAATCC TACAAATTTT 
AACCATGTGG CTCATATGGG ACCTGGAGAT GGCATGCAGA TACTCAGAGA CCTGCCAATG 
AATGTACGTG CTCAGGACAA CCGTGCTGGA TTCAGCGGCT CCGTCAGCAT CCCCTCCATT 
ACAAAAAACA GAGCAGAACC AGGGCGATCC ATGAGCGCAA GCAGTGGCCT AGGAATGCGG 
TCGTCCTCTC AGAACGGCAG TGCTCTAAGG AGGGAGCTGT CCAGCGGCAG CTACAGCTCT 
AAACGGCAGA CCATGACTTC ACCCTCCGAG AGTTCGCTGT CATCAGGAGG AGGGATGGAC 
GTGGGGGAAG CGCCGCTCTC TCAGTTTGAC AGAGAGGACT CTGATTCGCC GCGGCACTCC 
ACTGCCTCCA ACAGCTCCAC CTTCAGCAGC CCGCCGAGCC CCGCCTCCCC ACACAAGACA 
AAGTCCCTCT CATTGGAGAG CACAGACCGC ACCGCCTGGG ACACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-89p3.3 ( XM_017352506.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207995.1 Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X5, mRNA.

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CloneID	ODa56577
Clone ID Related Accession (Same CDS sequence)	XM_017352506.2 , XM_017352506.1
Accession Version	XM_017352506.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase MRCK alpha isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352506.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC 
TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG 
CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC 
CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT 
GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC 
TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA 
GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC 
GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA 
GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA 
CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT 
GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG 
ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC 
CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA 
GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG 
AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG 
AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC 
AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC 
AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT 
CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG 
CTCGAGGGCA TGAAGTTGAA GCAGACTGGG CCTCCTAACG GAGCTGCAAG CTCAGACCAG 
CAGCAGGAGC TGATGCGCGT TCGGGCTGAA CTGGAGAAGA AGAGCGTACA GTACGAGGAG 
GAGATGTGTC GCAGAGAGAC GCTCCACAGC AGTGAGATGA AGAACCTCCA AAAAGAGCTA 
CGGGATGCCG AGGGTCAACA CCTCACACAT CAGAAAGAGA TACTCGTGCT TAAAGACAAA 
CTGGAGAAAA CTCGCAGAGA AAGTCAAGTC GAGCGAGAAG AATTTGTGTG CGAGTACAAG 
CAGAAGTACG AGAGGGAAAA AGCCCATCTG ACAGACGAAA ACAAGAAGCT GAGTGCCGAA 
GTGGAGAAGT TGAACGGTCA GTTCGAGCAG CTGAGCAGCA GTCACCGGCA GCTGGAGGAG 
GAGATGAGAG AACTGGCTGA TAAGAAGGAG AGTGTTGCCC ACTGGGAGGC CCAGATCACC 
GAGATCATCC AGTGGGTGAG CGATGAGAAG GATGCCCGTG GTTACCTTCA AGCTCTGGCC 
ACCAAAATGA CAGAGGAGCT GGAAGGGCTC AGAAACACCA GTTTGGGAGC CAGAGGAACG 
GACATGCCGT GGAAGATGAG GCGTTTCGCC AAGCTGGACA TGTCTGCGCG TCTGGAGCTG 
CAGTCGGCGC TGGATACTGA GATCAGAGCC AAACAGTCCA TCCAAGACGA GCTTAACAAA 
GTCAAAGCAA CCAACATTGC CACAGAATGC AAATTGCAAG AATCCGAAAG TAGAAACCAG 
GAGCTGCTGG GAGAAATTGA GAGACTGAGG AAGGAGACAG AGGAGCTAAG GCTGAGGAGA 
GGCGTGAAGC ACCAGGATTC CCAAAATTCC TTTTTGGCTT TCCTCAATGC GCCCACTTCT 
GCACTGGATC AGTTTGATCG CTCACCCTCT TTTGGTCCCG GAAGCAAAAC CAGACGTCTT 
GACTCTGCAG ATTTCACCCC TTCCAACACT CCATCCAGAG ATGAAGACGC AAGATCTCGC 
CGTATTTCAC GCTCTCGCTC ACCCTCCACC GCCAGTGACA TGGAGCCTAT AGAGTTGATT 
GACCACACAT CACGTAGTCT TCAGACCCCA ACAGGCAGAT CAGGCTACGG GAGCTTGGGA 
CACTCATCAC CTAAGCCCAA GGCACACCAG TTTAGTGTAA AGTCCTTCAA TGTTCCCACT 
AAATGTAACC AGTGCACCTC TCTCATGGTG GGACTCATCC GTCAGGGCTG CACATGCGAA 
GCCTGCAGCT TCTCCTGTCA TGTCACTTGT GCTGATAAAG CCCCTTCTGT GTGCCCCGTC 
GCACCAGACC AGACCAGGGG CAAACTGGGC ATTGACACTC AGAGGGGCAT TGGGACTGCA 
TATGAGGGGC ATCTTAGGGT GCCGAAGCCC ACTGGGGTGA AGAAGGGTTG GCAGAGGGTC 
TGGGCAGTGG TTTGTGACTT CAAACTTTTT CTGTATGAGC TTGGAGAAGG GAAAGGTGCT 
CAGCCTGGTG TTGTAGTAAA CCAGGTCATT GACATGAGGG ATGAAGAGTT TTCCGCCAGT 
TCGGTTCTGG AGTCTGATGT CATCCATGCT AATAGGAAGG ATATACCCTG TATTTTTAGG 
GTAACAGCAT CTCAGCTGTC CGCCTCCTCC AGTCAGAAGA CGTGTATTCT AATTCTGGCT 
GACAACGAGC AAGAAAGGAC GAAGTGGGTG TGCGCTCTGA ATGAACTGCA TCGCATCCTG 
AGGAAAAACA AGCTGAAGGA GCGTTTCGTC TATGTGCCTA AAGAAGCCTA CGACAGCACA 
CTTCCTCTCA TCAAAACCAC CCAAACTGCT GCTATTATAG ATCATGAACG CGTTGCTTTG 
GGCAATGAGG AGGGCCTGTT TGTCATCCAC GTCACCAAAG ATGAAATCAT TCGTGTGGGT 
GATGTCAAGA AGGTGCATCA TGTAGAACTG ATGCCCACGG AGCACATACT GGCCGTCATC 
TCGGGCAGGA ACCGACAAGT ATGTCTGGTC TCCATGGCGG GTCTCGACGG GAGGGAGGTC 
GACAAGAATA AGGTGGCAGA TACTAAAAAC TGCCAGTTGC TGGTGTCCGG TGTGGTTCGC 
AATGTCACCT GCTTCTGCCT CGCCATTAAA CGGCAGATCT CATGCTTCGA AATTAACAAG 
AGCAAGTCAC GACACTCCCA TCTCGTGGAC ATACAAGCTC CAGGAACTGT CCAATGGATG 
GCGCTGTTCA ACCAGCAGTT GTGTGTTGGT TACCCATCAG GCTTCATGCG CTACAGTTTG 
TATGGAGAAA CACCTCCAGC CAGCTTACTT CATCCAGATG ACCCCACACT GGCATTTATT 
AAAACAGACA ACCTGGATGC CCTTTGTGCT GTGGAAATCT CCAATAAGGA ACTGCTGCTC 
TGCTTCAGTA AAATAGCAGT GTATGTTGAC ACACACGGCA GACGCTCGCG CCAGCAGGAG 
CTGATGTGGC CCGCGGCGCC CACCGCCTGC TGCTATAACG CCCCCTATCT GTCGGTCTAC 
AGTGAAAATG CAGTGGATGT GTTTGATGTG AACTCTACGG AATGGATTCA GACCATTCCT 
CTCAAAAAGG TCCGGCCATT AAACATGGAT GGCTCTTTGA ATCTCCTGGG CCTAGAAACG 
GTCAGACTCA TCTACTTCAG AAACAAGACC GCAGAGGGGG ATGAACTGGT GGTGCCTGAA 
GTGTGTGATA ACAGCAGAAA GCAGATGGTG CGAAGCATGA GCAACAAGAG ACGATTCTCG 
TTTCGTATTC CTGAGGAGGA GAGACTGCAG CAGAGGAGAG AAATGTTGAA AGATCCTGAG 
AGGAGGAACA AGCTGATCTC AAATCCTACA AATTTTAACC ATGTGGCTCA TATGGGACCT 
GGAGATGGCA TGCAGATACT CAGAGACCTG CCAATGAATG TACGTGCTCA GGACAACCGT 
GCTGGATTCA GCGGCTCCGT CAGCATCCCC TCCATTACAA AAAACAGAGC AGAACCAGGG 
CGATCCATGA GCGCAAGCAG TGGCCTAGGA ATGCGGTCGT CCTCTCAGAA CGGCAGTGCT 
CTAAGGAGGG AGCTGTCCAG CGGCAGCTAC AGCTCTAAAC GGCAGACCAT GACTTCACCC 
TCCGAGAGTT CGCTGTCATC AGGAGGAGGG ATGGACGTGG GGGAAGCGCC GCTCTCTCAG 
TTTGACAGAG AGTGGCAGGC AGTATCGCCC TCGGAGAAGA CCCCTGATCT GAAAAGGGCT 
TTAAGGACCC TGCTTAGTAA GATGAAGGAC TCTGATTCGC CGCGGCACTC CACTGCCTCC 
AACAGCTCCA CCTTCAGCAG CCCGCCGAGC CCCGCCTCCC CACACAAGAC AAAGTCCCTC 
TCATTGGAGA GCACAGACCG CACCGCCTGG GACACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207995.1
CDS	1641..7019
Translation	MSVEVRLKKLEKLIMDGPSQSSGQCFSMETLLDILVCLYDECSSSPIRREKNVLEFLDWAKPFTSKVKQMRLHKEDFEILKVIGRGAFGEVAVVKVKNTDKVFAMKILNKWEMLKRAETACFREERDVLVNGDCQWITTLHYAFQDENNLYLVMDYYVGGDLLTLLSKFEDRLPEDMAKFYLAEMVLAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPLQVTDVSEDAKDLVRRLICSREHRLGQNGIEDFKQHPFFTGIDWDNIRTCEAPYIPEVSSPTDTSNFDVDDDCLKNCETLPPPSHTAFSGHHLPFVGFTYTSKCTLSDRGCLRESVGPAQVDAHVQRSLEESLASEAYERRIRRLEQEKTELTRKLQESTQTVQALQYPDSDAPVNANKEVEIRSLKSEIDILKKQIADSGQMEQQLENASTARRDLEDSSKVVRNLEKQLKSVTQEKDDLHKELLGLAEKVKAQGKELKDAHSQKKMAIKEFAELNEQLSDVRTQKQRLSRQFKDKEEEMEGVSQKLEALRLEIRKAERMRKELEAQAEEHAAEAQKERKVRERTEQYSKQLEEELEGMKLKQTGPPNGAASSDQQQELMRVRAELEKKSVQYEEEMCRRETLHSSEMKNLQKELRDAEGQHLTHQKEILVLKDKLEKTRRESQVEREEFVCEYKQKYEREKAHLTDENKKLSAEVEKLNGQFEQLSSSHRQLEEEMRELADKKESVAHWEAQITEIIQWVSDEKDARGYLQALATKMTEELEGLRNTSLGARGTDMPWKMRRFAKLDMSARLELQSALDTEIRAKQSIQDELNKVKATNIATECKLQESESRNQELLGEIERLRKETEELRLRRGVKHQDSQNSFLAFLNAPTSALDQFDRSPSFGPGSKTRRLDSADFTPSNTPSRDEDARSRRISRSRSPSTASDMEPIELIDHTSRSLQTPTGRSGYGSLGHSSPKPKAHQFSVKSFNVPTKCNQCTSLMVGLIRQGCTCEACSFSCHVTCADKAPSVCPVAPDQTRGKLGIDTQRGIGTAYEGHLRVPKPTGVKKGWQRVWAVVCDFKLFLYELGEGKGAQPGVVVNQVIDMRDEEFSASSVLESDVIHANRKDIPCIFRVTASQLSASSSQKTCILILADNEQERTKWVCALNELHRILRKNKLKERFVYVPKEAYDSTLPLIKTTQTAAIIDHERVALGNEEGLFVIHVTKDEIIRVGDVKKVHHVELMPTEHILAVISGRNRQVCLVSMAGLDGREVDKNKVADTKNCQLLVSGVVRNVTCFCLAIKRQISCFEINKSKSRHSHLVDIQAPGTVQWMALFNQQLCVGYPSGFMRYSLYGETPPASLLHPDDPTLAFIKTDNLDALCAVEISNKELLLCFSKIAVYVDTHGRRSRQQELMWPAAPTACCYNAPYLSVYSENAVDVFDVNSTEWIQTIPLKKVRPLNMDGSLNLLGLETVRLIYFRNKTAEGDELVVPEVCDNSRKQMVRSMSNKRRFSFRIPEEERLQQRREMLKDPERRNKLISNPTNFNHVAHMGPGDGMQILRDLPMNVRAQDNRAGFSGSVSIPSITKNRAEPGRSMSASSGLGMRSSSQNGSALRRELSSGSYSSKRQTMTSPSESSLSSGGGMDVGEAPLSQFDREWQAVSPSEKTPDLKRALRTLLSKMKDSDSPRHSTASNSSTFSSPPSPASPHKTKSLSLESTDRTAWDT

Target ORF information:

RefSeq Version	XM_017352506.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352506.2

ORF Insert Sequence:

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC 
TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG 
CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC 
CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT 
GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC 
TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA 
GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC 
GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA 
GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA 
CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT 
GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG 
ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC 
CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA 
GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG 
AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG 
AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC 
AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC 
AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT 
CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG 
CTCGAGGGCA TGAAGTTGAA GCAGACTGGG CCTCCTAACG GAGCTGCAAG CTCAGACCAG 
CAGCAGGAGC TGATGCGCGT TCGGGCTGAA CTGGAGAAGA AGAGCGTACA GTACGAGGAG 
GAGATGTGTC GCAGAGAGAC GCTCCACAGC AGTGAGATGA AGAACCTCCA AAAAGAGCTA 
CGGGATGCCG AGGGTCAACA CCTCACACAT CAGAAAGAGA TACTCGTGCT TAAAGACAAA 
CTGGAGAAAA CTCGCAGAGA AAGTCAAGTC GAGCGAGAAG AATTTGTGTG CGAGTACAAG 
CAGAAGTACG AGAGGGAAAA AGCCCATCTG ACAGACGAAA ACAAGAAGCT GAGTGCCGAA 
GTGGAGAAGT TGAACGGTCA GTTCGAGCAG CTGAGCAGCA GTCACCGGCA GCTGGAGGAG 
GAGATGAGAG AACTGGCTGA TAAGAAGGAG AGTGTTGCCC ACTGGGAGGC CCAGATCACC 
GAGATCATCC AGTGGGTGAG CGATGAGAAG GATGCCCGTG GTTACCTTCA AGCTCTGGCC 
ACCAAAATGA CAGAGGAGCT GGAAGGGCTC AGAAACACCA GTTTGGGAGC CAGAGGAACG 
GACATGCCGT GGAAGATGAG GCGTTTCGCC AAGCTGGACA TGTCTGCGCG TCTGGAGCTG 
CAGTCGGCGC TGGATACTGA GATCAGAGCC AAACAGTCCA TCCAAGACGA GCTTAACAAA 
GTCAAAGCAA CCAACATTGC CACAGAATGC AAATTGCAAG AATCCGAAAG TAGAAACCAG 
GAGCTGCTGG GAGAAATTGA GAGACTGAGG AAGGAGACAG AGGAGCTAAG GCTGAGGAGA 
GGCGTGAAGC ACCAGGATTC CCAAAATTCC TTTTTGGCTT TCCTCAATGC GCCCACTTCT 
GCACTGGATC AGTTTGATCG CTCACCCTCT TTTGGTCCCG GAAGCAAAAC CAGACGTCTT 
GACTCTGCAG ATTTCACCCC TTCCAACACT CCATCCAGAG ATGAAGACGC AAGATCTCGC 
CGTATTTCAC GCTCTCGCTC ACCCTCCACC GCCAGTGACA TGGAGCCTAT AGAGTTGATT 
GACCACACAT CACGTAGTCT TCAGACCCCA ACAGGCAGAT CAGGCTACGG GAGCTTGGGA 
CACTCATCAC CTAAGCCCAA GGCACACCAG TTTAGTGTAA AGTCCTTCAA TGTTCCCACT 
AAATGTAACC AGTGCACCTC TCTCATGGTG GGACTCATCC GTCAGGGCTG CACATGCGAA 
GCCTGCAGCT TCTCCTGTCA TGTCACTTGT GCTGATAAAG CCCCTTCTGT GTGCCCCGTC 
GCACCAGACC AGACCAGGGG CAAACTGGGC ATTGACACTC AGAGGGGCAT TGGGACTGCA 
TATGAGGGGC ATCTTAGGGT GCCGAAGCCC ACTGGGGTGA AGAAGGGTTG GCAGAGGGTC 
TGGGCAGTGG TTTGTGACTT CAAACTTTTT CTGTATGAGC TTGGAGAAGG GAAAGGTGCT 
CAGCCTGGTG TTGTAGTAAA CCAGGTCATT GACATGAGGG ATGAAGAGTT TTCCGCCAGT 
TCGGTTCTGG AGTCTGATGT CATCCATGCT AATAGGAAGG ATATACCCTG TATTTTTAGG 
GTAACAGCAT CTCAGCTGTC CGCCTCCTCC AGTCAGAAGA CGTGTATTCT AATTCTGGCT 
GACAACGAGC AAGAAAGGAC GAAGTGGGTG TGCGCTCTGA ATGAACTGCA TCGCATCCTG 
AGGAAAAACA AGCTGAAGGA GCGTTTCGTC TATGTGCCTA AAGAAGCCTA CGACAGCACA 
CTTCCTCTCA TCAAAACCAC CCAAACTGCT GCTATTATAG ATCATGAACG CGTTGCTTTG 
GGCAATGAGG AGGGCCTGTT TGTCATCCAC GTCACCAAAG ATGAAATCAT TCGTGTGGGT 
GATGTCAAGA AGGTGCATCA TGTAGAACTG ATGCCCACGG AGCACATACT GGCCGTCATC 
TCGGGCAGGA ACCGACAAGT ATGTCTGGTC TCCATGGCGG GTCTCGACGG GAGGGAGGTC 
GACAAGAATA AGGTGGCAGA TACTAAAAAC TGCCAGTTGC TGGTGTCCGG TGTGGTTCGC 
AATGTCACCT GCTTCTGCCT CGCCATTAAA CGGCAGATCT CATGCTTCGA AATTAACAAG 
AGCAAGTCAC GACACTCCCA TCTCGTGGAC ATACAAGCTC CAGGAACTGT CCAATGGATG 
GCGCTGTTCA ACCAGCAGTT GTGTGTTGGT TACCCATCAG GCTTCATGCG CTACAGTTTG 
TATGGAGAAA CACCTCCAGC CAGCTTACTT CATCCAGATG ACCCCACACT GGCATTTATT 
AAAACAGACA ACCTGGATGC CCTTTGTGCT GTGGAAATCT CCAATAAGGA ACTGCTGCTC 
TGCTTCAGTA AAATAGCAGT GTATGTTGAC ACACACGGCA GACGCTCGCG CCAGCAGGAG 
CTGATGTGGC CCGCGGCGCC CACCGCCTGC TGCTATAACG CCCCCTATCT GTCGGTCTAC 
AGTGAAAATG CAGTGGATGT GTTTGATGTG AACTCTACGG AATGGATTCA GACCATTCCT 
CTCAAAAAGG TCCGGCCATT AAACATGGAT GGCTCTTTGA ATCTCCTGGG CCTAGAAACG 
GTCAGACTCA TCTACTTCAG AAACAAGACC GCAGAGGGGG ATGAACTGGT GGTGCCTGAA 
GTGTGTGATA ACAGCAGAAA GCAGATGGTG CGAAGCATGA GCAACAAGAG ACGATTCTCG 
TTTCGTATTC CTGAGGAGGA GAGACTGCAG CAGAGGAGAG AAATGTTGAA AGATCCTGAG 
AGGAGGAACA AGCTGATCTC AAATCCTACA AATTTTAACC ATGTGGCTCA TATGGGACCT 
GGAGATGGCA TGCAGATACT CAGAGACCTG CCAATGAATG TACGTGCTCA GGACAACCGT 
GCTGGATTCA GCGGCTCCGT CAGCATCCCC TCCATTACAA AAAACAGAGC AGAACCAGGG 
CGATCCATGA GCGCAAGCAG TGGCCTAGGA ATGCGGTCGT CCTCTCAGAA CGGCAGTGCT 
CTAAGGAGGG AGCTGTCCAG CGGCAGCTAC AGCTCTAAAC GGCAGACCAT GACTTCACCC 
TCCGAGAGTT CGCTGTCATC AGGAGGAGGG ATGGACGTGG GGGAAGCGCC GCTCTCTCAG 
TTTGACAGAG AGTGGCAGGC AGTATCGCCC TCGGAGAAGA CCCCTGATCT GAAAAGGGCT 
TTAAGGACCC TGCTTAGTAA GATGAAGGAC TCTGATTCGC CGCGGCACTC CACTGCCTCC 
AACAGCTCCA CCTTCAGCAG CCCGCCGAGC CCCGCCTCCC CACACAAGAC AAAGTCCCTC 
TCATTGGAGA GCACAGACCG CACCGCCTGG GACACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-89p3.3 ( XM_017352506.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207995.1 Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X2, mRNA.

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CloneID	ODa56577
Clone ID Related Accession (Same CDS sequence)	XM_017352506.2 , XM_017352506.1
Accession Version	XM_017352506.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase MRCK alpha isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC 
TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG 
CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC 
CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT 
GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC 
TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA 
GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC 
GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA 
GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA 
CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT 
GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG 
ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC 
CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA 
GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG 
AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG 
AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC 
AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC 
AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT 
CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG 
CTCGAGGGCA TGAAGTTGAA GCAGACTGGG CCTCCTAACG GAGCTGCAAG CTCAGACCAG 
CAGCAGGAGC TGATGCGCGT TCGGGCTGAA CTGGAGAAGA AGAGCGTACA GTACGAGGAG 
GAGATGTGTC GCAGAGAGAC GCTCCACAGC AGTGAGATGA AGAACCTCCA AAAAGAGCTA 
CGGGATGCCG AGGGTCAACA CCTCACACAT CAGAAAGAGA TACTCGTGCT TAAAGACAAA 
CTGGAGAAAA CTCGCAGAGA AAGTCAAGTC GAGCGAGAAG AATTTGTGTG CGAGTACAAG 
CAGAAGTACG AGAGGGAAAA AGCCCATCTG ACAGACGAAA ACAAGAAGCT GAGTGCCGAA 
GTGGAGAAGT TGAACGGTCA GTTCGAGCAG CTGAGCAGCA GTCACCGGCA GCTGGAGGAG 
GAGATGAGAG AACTGGCTGA TAAGAAGGAG AGTGTTGCCC ACTGGGAGGC CCAGATCACC 
GAGATCATCC AGTGGGTGAG CGATGAGAAG GATGCCCGTG GTTACCTTCA AGCTCTGGCC 
ACCAAAATGA CAGAGGAGCT GGAAGGGCTC AGAAACACCA GTTTGGGAGC CAGAGGAACG 
GACATGCCGT GGAAGATGAG GCGTTTCGCC AAGCTGGACA TGTCTGCGCG TCTGGAGCTG 
CAGTCGGCGC TGGATACTGA GATCAGAGCC AAACAGTCCA TCCAAGACGA GCTTAACAAA 
GTCAAAGCAA CCAACATTGC CACAGAATGC AAATTGCAAG AATCCGAAAG TAGAAACCAG 
GAGCTGCTGG GAGAAATTGA GAGACTGAGG AAGGAGACAG AGGAGCTAAG GCTGAGGAGA 
GGCGTGAAGC ACCAGGATTC CCAAAATTCC TTTTTGGCTT TCCTCAATGC GCCCACTTCT 
GCACTGGATC AGTTTGATCG CTCACCCTCT TTTGGTCCCG GAAGCAAAAC CAGACGTCTT 
GACTCTGCAG ATTTCACCCC TTCCAACACT CCATCCAGAG ATGAAGACGC AAGATCTCGC 
CGTATTTCAC GCTCTCGCTC ACCCTCCACC GCCAGTGACA TGGAGCCTAT AGAGTTGATT 
GACCACACAT CACGTAGTCT TCAGACCCCA ACAGGCAGAT CAGGCTACGG GAGCTTGGGA 
CACTCATCAC CTAAGCCCAA GGCACACCAG TTTAGTGTAA AGTCCTTCAA TGTTCCCACT 
AAATGTAACC AGTGCACCTC TCTCATGGTG GGACTCATCC GTCAGGGCTG CACATGCGAA 
GCCTGCAGCT TCTCCTGTCA TGTCACTTGT GCTGATAAAG CCCCTTCTGT GTGCCCCGTC 
GCACCAGACC AGACCAGGGG CAAACTGGGC ATTGACACTC AGAGGGGCAT TGGGACTGCA 
TATGAGGGGC ATCTTAGGGT GCCGAAGCCC ACTGGGGTGA AGAAGGGTTG GCAGAGGGTC 
TGGGCAGTGG TTTGTGACTT CAAACTTTTT CTGTATGAGC TTGGAGAAGG GAAAGGTGCT 
CAGCCTGGTG TTGTAGTAAA CCAGGTCATT GACATGAGGG ATGAAGAGTT TTCCGCCAGT 
TCGGTTCTGG AGTCTGATGT CATCCATGCT AATAGGAAGG ATATACCCTG TATTTTTAGG 
GTAACAGCAT CTCAGCTGTC CGCCTCCTCC AGTCAGAAGA CGTGTATTCT AATTCTGGCT 
GACAACGAGC AAGAAAGGAC GAAGTGGGTG TGCGCTCTGA ATGAACTGCA TCGCATCCTG 
AGGAAAAACA AGCTGAAGGA GCGTTTCGTC TATGTGCCTA AAGAAGCCTA CGACAGCACA 
CTTCCTCTCA TCAAAACCAC CCAAACTGCT GCTATTATAG ATCATGAACG CGTTGCTTTG 
GGCAATGAGG AGGGCCTGTT TGTCATCCAC GTCACCAAAG ATGAAATCAT TCGTGTGGGT 
GATGTCAAGA AGGTGCATCA TGTAGAACTG ATGCCCACGG AGCACATACT GGCCGTCATC 
TCGGGCAGGA ACCGACAAGT ATGTCTGGTC TCCATGGCGG GTCTCGACGG GAGGGAGGTC 
GACAAGAATA AGGTGGCAGA TACTAAAAAC TGCCAGTTGC TGGTGTCCGG TGTGGTTCGC 
AATGTCACCT GCTTCTGCCT CGCCATTAAA CGGCAGATCT CATGCTTCGA AATTAACAAG 
AGCAAGTCAC GACACTCCCA TCTCGTGGAC ATACAAGCTC CAGGAACTGT CCAATGGATG 
GCGCTGTTCA ACCAGCAGTT GTGTGTTGGT TACCCATCAG GCTTCATGCG CTACAGTTTG 
TATGGAGAAA CACCTCCAGC CAGCTTACTT CATCCAGATG ACCCCACACT GGCATTTATT 
AAAACAGACA ACCTGGATGC CCTTTGTGCT GTGGAAATCT CCAATAAGGA ACTGCTGCTC 
TGCTTCAGTA AAATAGCAGT GTATGTTGAC ACACACGGCA GACGCTCGCG CCAGCAGGAG 
CTGATGTGGC CCGCGGCGCC CACCGCCTGC TGCTATAACG CCCCCTATCT GTCGGTCTAC 
AGTGAAAATG CAGTGGATGT GTTTGATGTG AACTCTACGG AATGGATTCA GACCATTCCT 
CTCAAAAAGG TCCGGCCATT AAACATGGAT GGCTCTTTGA ATCTCCTGGG CCTAGAAACG 
GTCAGACTCA TCTACTTCAG AAACAAGACC GCAGAGGGGG ATGAACTGGT GGTGCCTGAA 
GTGTGTGATA ACAGCAGAAA GCAGATGGTG CGAAGCATGA GCAACAAGAG ACGATTCTCG 
TTTCGTATTC CTGAGGAGGA GAGACTGCAG CAGAGGAGAG AAATGTTGAA AGATCCTGAG 
AGGAGGAACA AGCTGATCTC AAATCCTACA AATTTTAACC ATGTGGCTCA TATGGGACCT 
GGAGATGGCA TGCAGATACT CAGAGACCTG CCAATGAATG TACGTGCTCA GGACAACCGT 
GCTGGATTCA GCGGCTCCGT CAGCATCCCC TCCATTACAA AAAACAGAGC AGAACCAGGG 
CGATCCATGA GCGCAAGCAG TGGCCTAGGA ATGCGGTCGT CCTCTCAGAA CGGCAGTGCT 
CTAAGGAGGG AGCTGTCCAG CGGCAGCTAC AGCTCTAAAC GGCAGACCAT GACTTCACCC 
TCCGAGAGTT CGCTGTCATC AGGAGGAGGG ATGGACGTGG GGGAAGCGCC GCTCTCTCAG 
TTTGACAGAG AGTGGCAGGC AGTATCGCCC TCGGAGAAGA CCCCTGATCT GAAAAGGGCT 
TTAAGGACCC TGCTTAGTAA GATGAAGGAC TCTGATTCGC CGCGGCACTC CACTGCCTCC 
AACAGCTCCA CCTTCAGCAG CCCGCCGAGC CCCGCCTCCC CACACAAGAC AAAGTCCCTC 
TCATTGGAGA GCACAGACCG CACCGCCTGG GACACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207995.1
CDS	1650..7028
Translation	MSVEVRLKKLEKLIMDGPSQSSGQCFSMETLLDILVCLYDECSSSPIRREKNVLEFLDWAKPFTSKVKQMRLHKEDFEILKVIGRGAFGEVAVVKVKNTDKVFAMKILNKWEMLKRAETACFREERDVLVNGDCQWITTLHYAFQDENNLYLVMDYYVGGDLLTLLSKFEDRLPEDMAKFYLAEMVLAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPLQVTDVSEDAKDLVRRLICSREHRLGQNGIEDFKQHPFFTGIDWDNIRTCEAPYIPEVSSPTDTSNFDVDDDCLKNCETLPPPSHTAFSGHHLPFVGFTYTSKCTLSDRGCLRESVGPAQVDAHVQRSLEESLASEAYERRIRRLEQEKTELTRKLQESTQTVQALQYPDSDAPVNANKEVEIRSLKSEIDILKKQIADSGQMEQQLENASTARRDLEDSSKVVRNLEKQLKSVTQEKDDLHKELLGLAEKVKAQGKELKDAHSQKKMAIKEFAELNEQLSDVRTQKQRLSRQFKDKEEEMEGVSQKLEALRLEIRKAERMRKELEAQAEEHAAEAQKERKVRERTEQYSKQLEEELEGMKLKQTGPPNGAASSDQQQELMRVRAELEKKSVQYEEEMCRRETLHSSEMKNLQKELRDAEGQHLTHQKEILVLKDKLEKTRRESQVEREEFVCEYKQKYEREKAHLTDENKKLSAEVEKLNGQFEQLSSSHRQLEEEMRELADKKESVAHWEAQITEIIQWVSDEKDARGYLQALATKMTEELEGLRNTSLGARGTDMPWKMRRFAKLDMSARLELQSALDTEIRAKQSIQDELNKVKATNIATECKLQESESRNQELLGEIERLRKETEELRLRRGVKHQDSQNSFLAFLNAPTSALDQFDRSPSFGPGSKTRRLDSADFTPSNTPSRDEDARSRRISRSRSPSTASDMEPIELIDHTSRSLQTPTGRSGYGSLGHSSPKPKAHQFSVKSFNVPTKCNQCTSLMVGLIRQGCTCEACSFSCHVTCADKAPSVCPVAPDQTRGKLGIDTQRGIGTAYEGHLRVPKPTGVKKGWQRVWAVVCDFKLFLYELGEGKGAQPGVVVNQVIDMRDEEFSASSVLESDVIHANRKDIPCIFRVTASQLSASSSQKTCILILADNEQERTKWVCALNELHRILRKNKLKERFVYVPKEAYDSTLPLIKTTQTAAIIDHERVALGNEEGLFVIHVTKDEIIRVGDVKKVHHVELMPTEHILAVISGRNRQVCLVSMAGLDGREVDKNKVADTKNCQLLVSGVVRNVTCFCLAIKRQISCFEINKSKSRHSHLVDIQAPGTVQWMALFNQQLCVGYPSGFMRYSLYGETPPASLLHPDDPTLAFIKTDNLDALCAVEISNKELLLCFSKIAVYVDTHGRRSRQQELMWPAAPTACCYNAPYLSVYSENAVDVFDVNSTEWIQTIPLKKVRPLNMDGSLNLLGLETVRLIYFRNKTAEGDELVVPEVCDNSRKQMVRSMSNKRRFSFRIPEEERLQQRREMLKDPERRNKLISNPTNFNHVAHMGPGDGMQILRDLPMNVRAQDNRAGFSGSVSIPSITKNRAEPGRSMSASSGLGMRSSSQNGSALRRELSSGSYSSKRQTMTSPSESSLSSGGGMDVGEAPLSQFDREWQAVSPSEKTPDLKRALRTLLSKMKDSDSPRHSTASNSSTFSSPPSPASPHKTKSLSLESTDRTAWDT

Target ORF information:

RefSeq Version	XM_017352506.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352506.1

ORF Insert Sequence:

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC 
TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG 
CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC 
CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT 
GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC 
TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA 
GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC 
GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA 
GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA 
CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT 
GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG 
ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC 
CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA 
GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG 
AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG 
AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC 
AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC 
AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT 
CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG 
CTCGAGGGCA TGAAGTTGAA GCAGACTGGG CCTCCTAACG GAGCTGCAAG CTCAGACCAG 
CAGCAGGAGC TGATGCGCGT TCGGGCTGAA CTGGAGAAGA AGAGCGTACA GTACGAGGAG 
GAGATGTGTC GCAGAGAGAC GCTCCACAGC AGTGAGATGA AGAACCTCCA AAAAGAGCTA 
CGGGATGCCG AGGGTCAACA CCTCACACAT CAGAAAGAGA TACTCGTGCT TAAAGACAAA 
CTGGAGAAAA CTCGCAGAGA AAGTCAAGTC GAGCGAGAAG AATTTGTGTG CGAGTACAAG 
CAGAAGTACG AGAGGGAAAA AGCCCATCTG ACAGACGAAA ACAAGAAGCT GAGTGCCGAA 
GTGGAGAAGT TGAACGGTCA GTTCGAGCAG CTGAGCAGCA GTCACCGGCA GCTGGAGGAG 
GAGATGAGAG AACTGGCTGA TAAGAAGGAG AGTGTTGCCC ACTGGGAGGC CCAGATCACC 
GAGATCATCC AGTGGGTGAG CGATGAGAAG GATGCCCGTG GTTACCTTCA AGCTCTGGCC 
ACCAAAATGA CAGAGGAGCT GGAAGGGCTC AGAAACACCA GTTTGGGAGC CAGAGGAACG 
GACATGCCGT GGAAGATGAG GCGTTTCGCC AAGCTGGACA TGTCTGCGCG TCTGGAGCTG 
CAGTCGGCGC TGGATACTGA GATCAGAGCC AAACAGTCCA TCCAAGACGA GCTTAACAAA 
GTCAAAGCAA CCAACATTGC CACAGAATGC AAATTGCAAG AATCCGAAAG TAGAAACCAG 
GAGCTGCTGG GAGAAATTGA GAGACTGAGG AAGGAGACAG AGGAGCTAAG GCTGAGGAGA 
GGCGTGAAGC ACCAGGATTC CCAAAATTCC TTTTTGGCTT TCCTCAATGC GCCCACTTCT 
GCACTGGATC AGTTTGATCG CTCACCCTCT TTTGGTCCCG GAAGCAAAAC CAGACGTCTT 
GACTCTGCAG ATTTCACCCC TTCCAACACT CCATCCAGAG ATGAAGACGC AAGATCTCGC 
CGTATTTCAC GCTCTCGCTC ACCCTCCACC GCCAGTGACA TGGAGCCTAT AGAGTTGATT 
GACCACACAT CACGTAGTCT TCAGACCCCA ACAGGCAGAT CAGGCTACGG GAGCTTGGGA 
CACTCATCAC CTAAGCCCAA GGCACACCAG TTTAGTGTAA AGTCCTTCAA TGTTCCCACT 
AAATGTAACC AGTGCACCTC TCTCATGGTG GGACTCATCC GTCAGGGCTG CACATGCGAA 
GCCTGCAGCT TCTCCTGTCA TGTCACTTGT GCTGATAAAG CCCCTTCTGT GTGCCCCGTC 
GCACCAGACC AGACCAGGGG CAAACTGGGC ATTGACACTC AGAGGGGCAT TGGGACTGCA 
TATGAGGGGC ATCTTAGGGT GCCGAAGCCC ACTGGGGTGA AGAAGGGTTG GCAGAGGGTC 
TGGGCAGTGG TTTGTGACTT CAAACTTTTT CTGTATGAGC TTGGAGAAGG GAAAGGTGCT 
CAGCCTGGTG TTGTAGTAAA CCAGGTCATT GACATGAGGG ATGAAGAGTT TTCCGCCAGT 
TCGGTTCTGG AGTCTGATGT CATCCATGCT AATAGGAAGG ATATACCCTG TATTTTTAGG 
GTAACAGCAT CTCAGCTGTC CGCCTCCTCC AGTCAGAAGA CGTGTATTCT AATTCTGGCT 
GACAACGAGC AAGAAAGGAC GAAGTGGGTG TGCGCTCTGA ATGAACTGCA TCGCATCCTG 
AGGAAAAACA AGCTGAAGGA GCGTTTCGTC TATGTGCCTA AAGAAGCCTA CGACAGCACA 
CTTCCTCTCA TCAAAACCAC CCAAACTGCT GCTATTATAG ATCATGAACG CGTTGCTTTG 
GGCAATGAGG AGGGCCTGTT TGTCATCCAC GTCACCAAAG ATGAAATCAT TCGTGTGGGT 
GATGTCAAGA AGGTGCATCA TGTAGAACTG ATGCCCACGG AGCACATACT GGCCGTCATC 
TCGGGCAGGA ACCGACAAGT ATGTCTGGTC TCCATGGCGG GTCTCGACGG GAGGGAGGTC 
GACAAGAATA AGGTGGCAGA TACTAAAAAC TGCCAGTTGC TGGTGTCCGG TGTGGTTCGC 
AATGTCACCT GCTTCTGCCT CGCCATTAAA CGGCAGATCT CATGCTTCGA AATTAACAAG 
AGCAAGTCAC GACACTCCCA TCTCGTGGAC ATACAAGCTC CAGGAACTGT CCAATGGATG 
GCGCTGTTCA ACCAGCAGTT GTGTGTTGGT TACCCATCAG GCTTCATGCG CTACAGTTTG 
TATGGAGAAA CACCTCCAGC CAGCTTACTT CATCCAGATG ACCCCACACT GGCATTTATT 
AAAACAGACA ACCTGGATGC CCTTTGTGCT GTGGAAATCT CCAATAAGGA ACTGCTGCTC 
TGCTTCAGTA AAATAGCAGT GTATGTTGAC ACACACGGCA GACGCTCGCG CCAGCAGGAG 
CTGATGTGGC CCGCGGCGCC CACCGCCTGC TGCTATAACG CCCCCTATCT GTCGGTCTAC 
AGTGAAAATG CAGTGGATGT GTTTGATGTG AACTCTACGG AATGGATTCA GACCATTCCT 
CTCAAAAAGG TCCGGCCATT AAACATGGAT GGCTCTTTGA ATCTCCTGGG CCTAGAAACG 
GTCAGACTCA TCTACTTCAG AAACAAGACC GCAGAGGGGG ATGAACTGGT GGTGCCTGAA 
GTGTGTGATA ACAGCAGAAA GCAGATGGTG CGAAGCATGA GCAACAAGAG ACGATTCTCG 
TTTCGTATTC CTGAGGAGGA GAGACTGCAG CAGAGGAGAG AAATGTTGAA AGATCCTGAG 
AGGAGGAACA AGCTGATCTC AAATCCTACA AATTTTAACC ATGTGGCTCA TATGGGACCT 
GGAGATGGCA TGCAGATACT CAGAGACCTG CCAATGAATG TACGTGCTCA GGACAACCGT 
GCTGGATTCA GCGGCTCCGT CAGCATCCCC TCCATTACAA AAAACAGAGC AGAACCAGGG 
CGATCCATGA GCGCAAGCAG TGGCCTAGGA ATGCGGTCGT CCTCTCAGAA CGGCAGTGCT 
CTAAGGAGGG AGCTGTCCAG CGGCAGCTAC AGCTCTAAAC GGCAGACCAT GACTTCACCC 
TCCGAGAGTT CGCTGTCATC AGGAGGAGGG ATGGACGTGG GGGAAGCGCC GCTCTCTCAG 
TTTGACAGAG AGTGGCAGGC AGTATCGCCC TCGGAGAAGA CCCCTGATCT GAAAAGGGCT 
TTAAGGACCC TGCTTAGTAA GATGAAGGAC TCTGATTCGC CGCGGCACTC CACTGCCTCC 
AACAGCTCCA CCTTCAGCAG CCCGCCGAGC CCCGCCTCCC CACACAAGAC AAAGTCCCTC 
TCATTGGAGA GCACAGACCG CACCGCCTGG GACACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-89p3.3 ( XM_017352507.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207996.1 Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X7, mRNA.

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CloneID	ODa56578
Clone ID Related Accession (Same CDS sequence)	XM_017352507.1 , XM_017352507.2
Accession Version	XM_017352507.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase MRCK alpha isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC 
TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG 
CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC 
CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT 
GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC 
TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA 
GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC 
GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA 
GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA 
CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT 
GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG 
ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC 
CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA 
GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG 
AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG 
AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC 
AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC 
AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT 
CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG 
CTCGAGGGCA TGAAGTTGAA GCAGACTGGG CCTCCTAACG GAGCTGCAAG CTCAGACCAG 
CAGCAGGAGC TGATGCGCGT TCGGGCTGAA CTGGAGAAGA AGAGCGTACA GTACGAGGAG 
GAGATGTGTC GCAGAGAGAC GCTCCACAGC AGTGAGATGA AGAACCTCCA AAAAGAGCTA 
CGGGATGCCG AGGGTCAACA CCTCACACAT CAGAAAGAGA TACTCGTGCT TAAAGACAAA 
CTGGAGAAAA CTCGCAGAGA AAGTCAAGTC GAGCGAGAAG AATTTGTGTG CGAGTACAAG 
CAGAAGTACG AGAGGGAAAA AGCCCATCTG ACAGACGAAA ACAAGAAGCT GAGTGCCGAA 
GTGGAGAAGT TGAACGGTCA GTTCGAGCAG CTGAGCAGCA GTCACCGGCA GCTGGAGGAG 
GAGATGAGAG AACTGGCTGA TAAGAAGGAG AGTGTTGCCC ACTGGGAGGC CCAGATCACC 
GAGATCATCC AGTGGGTGAG CGATGAGAAG GATGCCCGTG GTTACCTTCA AGCTCTGGCC 
ACCAAAATGA CAGAGGAGCT GGAAGGGCTC AGAAACACCA GTTTGGGAGC CAGAGGAACG 
GACATGCCGT GGAAGATGAG GCGTTTCGCC AAGCTGGACA TGTCTGCGCG TCTGGAGCTG 
CAGTCGGCGC TGGATACTGA GATCAGAGCC AAACAGTCCA TCCAAGACGA GCTTAACAAA 
GTCAAAGCAA CCAACATTGC CACAGAATGC AAATTGCAAG AATCCGAAAG TAGAAACCAG 
GAGCTGCTGG GAGAAATTGA GAGACTGAGG AAGGAGACAG AGGAGCTAAG GCTGAGGAGA 
GGCGTGAAGC ACCAGGATTC CCAAAATTCC TTTTTGGCTT TCCTCAATGC GCCCACTTCT 
GCACTGGATC AGTTTGATCG CTCACCCTCT TTTGGTCCCG GAAGCAAAAC CAGACGTCTT 
GACTCTGCAG ATTTCACCCC TTCCAACACT CCATCCAGAG ATGAAGACGC AAGATCTCGC 
CGTATTTCAC GCTCTCGCTC ACCCTCCACC GCCAGTGACA TGGAGCCTAT AGAGTTGATT 
GACCACACAT CACGTAGTCT TCAGACCCCA ACAGGCAGAT CAGGCTACGG GAGCTTGGGA 
CACTCATCAC CTAAGCCCAA GGCACACCAG TTTAGTGTAA AGTCCTTCAA TGTTCCCACT 
AAATGTAACC AGTGCACCTC TCTCATGGTG GGACTCATCC GTCAGGGCTG CACATGCGAA 
GCCTGCAGCT TCTCCTGTCA TGTCACTTGT GCTGATAAAG CCCCTTCTGT GTGCCCCGTC 
GCACCAGACC AGACCAGGGG CAAACTGGGC ATTGACACTC AGAGGGGCAT TGGGACTGCA 
TATGAGGGGC ATCTTAGGGT GCCGAAGCCC ACTGGGGTGA AGAAGGGTTG GCAGAGGGTC 
TGGGCAGTGG TTTGTGACTT CAAACTTTTT CTGTATGAGC TTGGAGAAGG GAAAGGTGCT 
CAGCCTGGTG TTGTAGTAAA CCAGGTCATT GACATGAGGG ATGAAGAGTT TTCCGCCAGT 
TCGGTTCTGG AGTCTGATGT CATCCATGCT AATAGGAAGG ATATACCCTG TATTTTTAGG 
GTAACAGCAT CTCAGCTGTC CGCCTCCTCC AGTCAGAAGA CGTGTATTCT AATTCTGGCT 
GACAACGAGC AAGAAAGGAC GAAGTGGGTG TGCGCTCTGA ATGAACTGCA TCGCATCCTG 
AGGAAAAACA AGCTGAAGGA GCGTTTCGTC TATGTGCCTA AAGAAGCCTA CGACAGCACA 
CTTCCTCTCA TCAAAACCAC CCAAACTGCT GCTATTATAG ATCATGAACG CGTTGCTTTG 
GGCAATGAGG AGGGCCTGTT TGTCATCCAC GTCACCAAAG ATGAAATCAT TCGTGTGGGT 
GATGTCAAGA AGGTGCATCA TGTAGAACTG ATGCCCACGG AGCACATACT GGCCGTCATC 
TCGGGCAGGA ACCGACAAGT ATGTCTGGTC TCCATGGCGG GTCTCGACGG GAGGGAGGTC 
GACAAGAATA AGGTGGCAGA TACTAAAAAC TGCCAGTTGC TGGTGTCCGG TGTGGTTCGC 
AATGTCACCT GCTTCTGCCT CGCCATTAAA CGGCAGATCT CATGCTTCGA AATTAACAAG 
AGCAAGTCAC GACACTCCCA TCTCGTGGAC ATACAAGCTC CAGGAACTGT CCAATGGATG 
GCGCTGTTCA ACCAGCAGTT GTGTGTTGGT TACCCATCAG GCTTCATGCG CTACAGTTTG 
TATGGAGAAA CACCTCCAGC CAGCTTACTT CATCCAGATG ACCCCACACT GGCATTTATT 
AAAACAGACA ACCTGGATGC CCTTTGTGCT GTGGAAATCT CCAATAAGGA ACTGCTGCTC 
TGCTTCAGTA AAATAGCAGT GTATGTTGAC ACACACGGCA GACGCTCGCG CCAGCAGGAG 
CTGATGTGGC CCGCGGCGCC CACCGCCTGC TGCTATAACG CCCCCTATCT GTCGGTCTAC 
AGTGAAAATG CAGTGGATGT GTTTGATGTG AACTCTACGG AATGGATTCA GACCATTCCT 
CTCAAAAAGG TCCGGCCATT AAACATGGAT GGCTCTTTGA ATCTCCTGGG CCTAGAAACG 
GTCAGACTCA TCTACTTCAG AAACAAGACC GCAGAGGGGG ATGAACTGGT GGTGCCTGAA 
GTGTGTGATA ACAGCAGAAA GCAGATGGTG CGAAGCATGA GCAACAAGAG ACGATTCTCG 
TTTCGTATTC CTGAGGAGGA GAGACTGCAG CAGAGGAGAG AAATGTTGAA AGATCCTGAG 
AGGAGGAACA AGCTGATCTC AAATCCTACA AATTTTAACC ATGTGGCTCA TATGGGACCT 
GGAGATGGCA TGCAGATACT CAGAGACCTG CCAATGAATG TACGTGCTCA GGACAACCGT 
GCTGGATTCA GCGGCTCCGT CAGCATCCCC TCCATTACAA AAAACAGAGC AGAACCAGGG 
CGATCCATGA GCGCAAGCAG TGGCCTAGGA ATGCGGTCGT CCTCTCAGAA CGGCAGTGCT 
CTAAGGAGGG AGCTGTCCAG CGGCAGCTAC AGCTCTAAAC GGCAGACCAT GACTTCACCC 
TCCGAGAGTT CGCTGTCATC AGGAGGAGGG ATGGACGTGG GGGAAGCGCC GCTCTCTCAG 
TTTGACAGAG AGGACTCTGA TTCGCCGCGG CACTCCACTG CCTCCAACAG CTCCACCTTC 
AGCAGCCCGC CGAGCCCCGC CTCCCCACAC AAGACAAAGT CCCTCTCATT GGAGAGCACA 
GACCGCACCG CCTGGGACAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207996.1
CDS	1650..6953
Translation	MSVEVRLKKLEKLIMDGPSQSSGQCFSMETLLDILVCLYDECSSSPIRREKNVLEFLDWAKPFTSKVKQMRLHKEDFEILKVIGRGAFGEVAVVKVKNTDKVFAMKILNKWEMLKRAETACFREERDVLVNGDCQWITTLHYAFQDENNLYLVMDYYVGGDLLTLLSKFEDRLPEDMAKFYLAEMVLAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPLQVTDVSEDAKDLVRRLICSREHRLGQNGIEDFKQHPFFTGIDWDNIRTCEAPYIPEVSSPTDTSNFDVDDDCLKNCETLPPPSHTAFSGHHLPFVGFTYTSKCTLSDRGCLRESVGPAQVDAHVQRSLEESLASEAYERRIRRLEQEKTELTRKLQESTQTVQALQYPDSDAPVNANKEVEIRSLKSEIDILKKQIADSGQMEQQLENASTARRDLEDSSKVVRNLEKQLKSVTQEKDDLHKELLGLAEKVKAQGKELKDAHSQKKMAIKEFAELNEQLSDVRTQKQRLSRQFKDKEEEMEGVSQKLEALRLEIRKAERMRKELEAQAEEHAAEAQKERKVRERTEQYSKQLEEELEGMKLKQTGPPNGAASSDQQQELMRVRAELEKKSVQYEEEMCRRETLHSSEMKNLQKELRDAEGQHLTHQKEILVLKDKLEKTRRESQVEREEFVCEYKQKYEREKAHLTDENKKLSAEVEKLNGQFEQLSSSHRQLEEEMRELADKKESVAHWEAQITEIIQWVSDEKDARGYLQALATKMTEELEGLRNTSLGARGTDMPWKMRRFAKLDMSARLELQSALDTEIRAKQSIQDELNKVKATNIATECKLQESESRNQELLGEIERLRKETEELRLRRGVKHQDSQNSFLAFLNAPTSALDQFDRSPSFGPGSKTRRLDSADFTPSNTPSRDEDARSRRISRSRSPSTASDMEPIELIDHTSRSLQTPTGRSGYGSLGHSSPKPKAHQFSVKSFNVPTKCNQCTSLMVGLIRQGCTCEACSFSCHVTCADKAPSVCPVAPDQTRGKLGIDTQRGIGTAYEGHLRVPKPTGVKKGWQRVWAVVCDFKLFLYELGEGKGAQPGVVVNQVIDMRDEEFSASSVLESDVIHANRKDIPCIFRVTASQLSASSSQKTCILILADNEQERTKWVCALNELHRILRKNKLKERFVYVPKEAYDSTLPLIKTTQTAAIIDHERVALGNEEGLFVIHVTKDEIIRVGDVKKVHHVELMPTEHILAVISGRNRQVCLVSMAGLDGREVDKNKVADTKNCQLLVSGVVRNVTCFCLAIKRQISCFEINKSKSRHSHLVDIQAPGTVQWMALFNQQLCVGYPSGFMRYSLYGETPPASLLHPDDPTLAFIKTDNLDALCAVEISNKELLLCFSKIAVYVDTHGRRSRQQELMWPAAPTACCYNAPYLSVYSENAVDVFDVNSTEWIQTIPLKKVRPLNMDGSLNLLGLETVRLIYFRNKTAEGDELVVPEVCDNSRKQMVRSMSNKRRFSFRIPEEERLQQRREMLKDPERRNKLISNPTNFNHVAHMGPGDGMQILRDLPMNVRAQDNRAGFSGSVSIPSITKNRAEPGRSMSASSGLGMRSSSQNGSALRRELSSGSYSSKRQTMTSPSESSLSSGGGMDVGEAPLSQFDREDSDSPRHSTASNSSTFSSPPSPASPHKTKSLSLESTDRTAWDT

Target ORF information:

RefSeq Version	XM_017352507.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352507.1

ORF Insert Sequence:

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC 
TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG 
CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC 
CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT 
GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC 
TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA 
GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC 
GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA 
GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA 
CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT 
GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG 
ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC 
CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA 
GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG 
AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG 
AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC 
AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC 
AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT 
CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG 
CTCGAGGGCA TGAAGTTGAA GCAGACTGGG CCTCCTAACG GAGCTGCAAG CTCAGACCAG 
CAGCAGGAGC TGATGCGCGT TCGGGCTGAA CTGGAGAAGA AGAGCGTACA GTACGAGGAG 
GAGATGTGTC GCAGAGAGAC GCTCCACAGC AGTGAGATGA AGAACCTCCA AAAAGAGCTA 
CGGGATGCCG AGGGTCAACA CCTCACACAT CAGAAAGAGA TACTCGTGCT TAAAGACAAA 
CTGGAGAAAA CTCGCAGAGA AAGTCAAGTC GAGCGAGAAG AATTTGTGTG CGAGTACAAG 
CAGAAGTACG AGAGGGAAAA AGCCCATCTG ACAGACGAAA ACAAGAAGCT GAGTGCCGAA 
GTGGAGAAGT TGAACGGTCA GTTCGAGCAG CTGAGCAGCA GTCACCGGCA GCTGGAGGAG 
GAGATGAGAG AACTGGCTGA TAAGAAGGAG AGTGTTGCCC ACTGGGAGGC CCAGATCACC 
GAGATCATCC AGTGGGTGAG CGATGAGAAG GATGCCCGTG GTTACCTTCA AGCTCTGGCC 
ACCAAAATGA CAGAGGAGCT GGAAGGGCTC AGAAACACCA GTTTGGGAGC CAGAGGAACG 
GACATGCCGT GGAAGATGAG GCGTTTCGCC AAGCTGGACA TGTCTGCGCG TCTGGAGCTG 
CAGTCGGCGC TGGATACTGA GATCAGAGCC AAACAGTCCA TCCAAGACGA GCTTAACAAA 
GTCAAAGCAA CCAACATTGC CACAGAATGC AAATTGCAAG AATCCGAAAG TAGAAACCAG 
GAGCTGCTGG GAGAAATTGA GAGACTGAGG AAGGAGACAG AGGAGCTAAG GCTGAGGAGA 
GGCGTGAAGC ACCAGGATTC CCAAAATTCC TTTTTGGCTT TCCTCAATGC GCCCACTTCT 
GCACTGGATC AGTTTGATCG CTCACCCTCT TTTGGTCCCG GAAGCAAAAC CAGACGTCTT 
GACTCTGCAG ATTTCACCCC TTCCAACACT CCATCCAGAG ATGAAGACGC AAGATCTCGC 
CGTATTTCAC GCTCTCGCTC ACCCTCCACC GCCAGTGACA TGGAGCCTAT AGAGTTGATT 
GACCACACAT CACGTAGTCT TCAGACCCCA ACAGGCAGAT CAGGCTACGG GAGCTTGGGA 
CACTCATCAC CTAAGCCCAA GGCACACCAG TTTAGTGTAA AGTCCTTCAA TGTTCCCACT 
AAATGTAACC AGTGCACCTC TCTCATGGTG GGACTCATCC GTCAGGGCTG CACATGCGAA 
GCCTGCAGCT TCTCCTGTCA TGTCACTTGT GCTGATAAAG CCCCTTCTGT GTGCCCCGTC 
GCACCAGACC AGACCAGGGG CAAACTGGGC ATTGACACTC AGAGGGGCAT TGGGACTGCA 
TATGAGGGGC ATCTTAGGGT GCCGAAGCCC ACTGGGGTGA AGAAGGGTTG GCAGAGGGTC 
TGGGCAGTGG TTTGTGACTT CAAACTTTTT CTGTATGAGC TTGGAGAAGG GAAAGGTGCT 
CAGCCTGGTG TTGTAGTAAA CCAGGTCATT GACATGAGGG ATGAAGAGTT TTCCGCCAGT 
TCGGTTCTGG AGTCTGATGT CATCCATGCT AATAGGAAGG ATATACCCTG TATTTTTAGG 
GTAACAGCAT CTCAGCTGTC CGCCTCCTCC AGTCAGAAGA CGTGTATTCT AATTCTGGCT 
GACAACGAGC AAGAAAGGAC GAAGTGGGTG TGCGCTCTGA ATGAACTGCA TCGCATCCTG 
AGGAAAAACA AGCTGAAGGA GCGTTTCGTC TATGTGCCTA AAGAAGCCTA CGACAGCACA 
CTTCCTCTCA TCAAAACCAC CCAAACTGCT GCTATTATAG ATCATGAACG CGTTGCTTTG 
GGCAATGAGG AGGGCCTGTT TGTCATCCAC GTCACCAAAG ATGAAATCAT TCGTGTGGGT 
GATGTCAAGA AGGTGCATCA TGTAGAACTG ATGCCCACGG AGCACATACT GGCCGTCATC 
TCGGGCAGGA ACCGACAAGT ATGTCTGGTC TCCATGGCGG GTCTCGACGG GAGGGAGGTC 
GACAAGAATA AGGTGGCAGA TACTAAAAAC TGCCAGTTGC TGGTGTCCGG TGTGGTTCGC 
AATGTCACCT GCTTCTGCCT CGCCATTAAA CGGCAGATCT CATGCTTCGA AATTAACAAG 
AGCAAGTCAC GACACTCCCA TCTCGTGGAC ATACAAGCTC CAGGAACTGT CCAATGGATG 
GCGCTGTTCA ACCAGCAGTT GTGTGTTGGT TACCCATCAG GCTTCATGCG CTACAGTTTG 
TATGGAGAAA CACCTCCAGC CAGCTTACTT CATCCAGATG ACCCCACACT GGCATTTATT 
AAAACAGACA ACCTGGATGC CCTTTGTGCT GTGGAAATCT CCAATAAGGA ACTGCTGCTC 
TGCTTCAGTA AAATAGCAGT GTATGTTGAC ACACACGGCA GACGCTCGCG CCAGCAGGAG 
CTGATGTGGC CCGCGGCGCC CACCGCCTGC TGCTATAACG CCCCCTATCT GTCGGTCTAC 
AGTGAAAATG CAGTGGATGT GTTTGATGTG AACTCTACGG AATGGATTCA GACCATTCCT 
CTCAAAAAGG TCCGGCCATT AAACATGGAT GGCTCTTTGA ATCTCCTGGG CCTAGAAACG 
GTCAGACTCA TCTACTTCAG AAACAAGACC GCAGAGGGGG ATGAACTGGT GGTGCCTGAA 
GTGTGTGATA ACAGCAGAAA GCAGATGGTG CGAAGCATGA GCAACAAGAG ACGATTCTCG 
TTTCGTATTC CTGAGGAGGA GAGACTGCAG CAGAGGAGAG AAATGTTGAA AGATCCTGAG 
AGGAGGAACA AGCTGATCTC AAATCCTACA AATTTTAACC ATGTGGCTCA TATGGGACCT 
GGAGATGGCA TGCAGATACT CAGAGACCTG CCAATGAATG TACGTGCTCA GGACAACCGT 
GCTGGATTCA GCGGCTCCGT CAGCATCCCC TCCATTACAA AAAACAGAGC AGAACCAGGG 
CGATCCATGA GCGCAAGCAG TGGCCTAGGA ATGCGGTCGT CCTCTCAGAA CGGCAGTGCT 
CTAAGGAGGG AGCTGTCCAG CGGCAGCTAC AGCTCTAAAC GGCAGACCAT GACTTCACCC 
TCCGAGAGTT CGCTGTCATC AGGAGGAGGG ATGGACGTGG GGGAAGCGCC GCTCTCTCAG 
TTTGACAGAG AGGACTCTGA TTCGCCGCGG CACTCCACTG CCTCCAACAG CTCCACCTTC 
AGCAGCCCGC CGAGCCCCGC CTCCCCACAC AAGACAAAGT CCCTCTCATT GGAGAGCACA 
GACCGCACCG CCTGGGACAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-89p3.3 ( XM_017352507.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207996.1 Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X9, mRNA.

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CloneID	ODa56578
Clone ID Related Accession (Same CDS sequence)	XM_017352507.1 , XM_017352507.2
Accession Version	XM_017352507.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase MRCK alpha isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352507.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC 
TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG 
CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC 
CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT 
GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC 
TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA 
GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC 
GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA 
GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA 
CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT 
GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG 
ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC 
CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA 
GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG 
AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG 
AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC 
AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC 
AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT 
CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG 
CTCGAGGGCA TGAAGTTGAA GCAGACTGGG CCTCCTAACG GAGCTGCAAG CTCAGACCAG 
CAGCAGGAGC TGATGCGCGT TCGGGCTGAA CTGGAGAAGA AGAGCGTACA GTACGAGGAG 
GAGATGTGTC GCAGAGAGAC GCTCCACAGC AGTGAGATGA AGAACCTCCA AAAAGAGCTA 
CGGGATGCCG AGGGTCAACA CCTCACACAT CAGAAAGAGA TACTCGTGCT TAAAGACAAA 
CTGGAGAAAA CTCGCAGAGA AAGTCAAGTC GAGCGAGAAG AATTTGTGTG CGAGTACAAG 
CAGAAGTACG AGAGGGAAAA AGCCCATCTG ACAGACGAAA ACAAGAAGCT GAGTGCCGAA 
GTGGAGAAGT TGAACGGTCA GTTCGAGCAG CTGAGCAGCA GTCACCGGCA GCTGGAGGAG 
GAGATGAGAG AACTGGCTGA TAAGAAGGAG AGTGTTGCCC ACTGGGAGGC CCAGATCACC 
GAGATCATCC AGTGGGTGAG CGATGAGAAG GATGCCCGTG GTTACCTTCA AGCTCTGGCC 
ACCAAAATGA CAGAGGAGCT GGAAGGGCTC AGAAACACCA GTTTGGGAGC CAGAGGAACG 
GACATGCCGT GGAAGATGAG GCGTTTCGCC AAGCTGGACA TGTCTGCGCG TCTGGAGCTG 
CAGTCGGCGC TGGATACTGA GATCAGAGCC AAACAGTCCA TCCAAGACGA GCTTAACAAA 
GTCAAAGCAA CCAACATTGC CACAGAATGC AAATTGCAAG AATCCGAAAG TAGAAACCAG 
GAGCTGCTGG GAGAAATTGA GAGACTGAGG AAGGAGACAG AGGAGCTAAG GCTGAGGAGA 
GGCGTGAAGC ACCAGGATTC CCAAAATTCC TTTTTGGCTT TCCTCAATGC GCCCACTTCT 
GCACTGGATC AGTTTGATCG CTCACCCTCT TTTGGTCCCG GAAGCAAAAC CAGACGTCTT 
GACTCTGCAG ATTTCACCCC TTCCAACACT CCATCCAGAG ATGAAGACGC AAGATCTCGC 
CGTATTTCAC GCTCTCGCTC ACCCTCCACC GCCAGTGACA TGGAGCCTAT AGAGTTGATT 
GACCACACAT CACGTAGTCT TCAGACCCCA ACAGGCAGAT CAGGCTACGG GAGCTTGGGA 
CACTCATCAC CTAAGCCCAA GGCACACCAG TTTAGTGTAA AGTCCTTCAA TGTTCCCACT 
AAATGTAACC AGTGCACCTC TCTCATGGTG GGACTCATCC GTCAGGGCTG CACATGCGAA 
GCCTGCAGCT TCTCCTGTCA TGTCACTTGT GCTGATAAAG CCCCTTCTGT GTGCCCCGTC 
GCACCAGACC AGACCAGGGG CAAACTGGGC ATTGACACTC AGAGGGGCAT TGGGACTGCA 
TATGAGGGGC ATCTTAGGGT GCCGAAGCCC ACTGGGGTGA AGAAGGGTTG GCAGAGGGTC 
TGGGCAGTGG TTTGTGACTT CAAACTTTTT CTGTATGAGC TTGGAGAAGG GAAAGGTGCT 
CAGCCTGGTG TTGTAGTAAA CCAGGTCATT GACATGAGGG ATGAAGAGTT TTCCGCCAGT 
TCGGTTCTGG AGTCTGATGT CATCCATGCT AATAGGAAGG ATATACCCTG TATTTTTAGG 
GTAACAGCAT CTCAGCTGTC CGCCTCCTCC AGTCAGAAGA CGTGTATTCT AATTCTGGCT 
GACAACGAGC AAGAAAGGAC GAAGTGGGTG TGCGCTCTGA ATGAACTGCA TCGCATCCTG 
AGGAAAAACA AGCTGAAGGA GCGTTTCGTC TATGTGCCTA AAGAAGCCTA CGACAGCACA 
CTTCCTCTCA TCAAAACCAC CCAAACTGCT GCTATTATAG ATCATGAACG CGTTGCTTTG 
GGCAATGAGG AGGGCCTGTT TGTCATCCAC GTCACCAAAG ATGAAATCAT TCGTGTGGGT 
GATGTCAAGA AGGTGCATCA TGTAGAACTG ATGCCCACGG AGCACATACT GGCCGTCATC 
TCGGGCAGGA ACCGACAAGT ATGTCTGGTC TCCATGGCGG GTCTCGACGG GAGGGAGGTC 
GACAAGAATA AGGTGGCAGA TACTAAAAAC TGCCAGTTGC TGGTGTCCGG TGTGGTTCGC 
AATGTCACCT GCTTCTGCCT CGCCATTAAA CGGCAGATCT CATGCTTCGA AATTAACAAG 
AGCAAGTCAC GACACTCCCA TCTCGTGGAC ATACAAGCTC CAGGAACTGT CCAATGGATG 
GCGCTGTTCA ACCAGCAGTT GTGTGTTGGT TACCCATCAG GCTTCATGCG CTACAGTTTG 
TATGGAGAAA CACCTCCAGC CAGCTTACTT CATCCAGATG ACCCCACACT GGCATTTATT 
AAAACAGACA ACCTGGATGC CCTTTGTGCT GTGGAAATCT CCAATAAGGA ACTGCTGCTC 
TGCTTCAGTA AAATAGCAGT GTATGTTGAC ACACACGGCA GACGCTCGCG CCAGCAGGAG 
CTGATGTGGC CCGCGGCGCC CACCGCCTGC TGCTATAACG CCCCCTATCT GTCGGTCTAC 
AGTGAAAATG CAGTGGATGT GTTTGATGTG AACTCTACGG AATGGATTCA GACCATTCCT 
CTCAAAAAGG TCCGGCCATT AAACATGGAT GGCTCTTTGA ATCTCCTGGG CCTAGAAACG 
GTCAGACTCA TCTACTTCAG AAACAAGACC GCAGAGGGGG ATGAACTGGT GGTGCCTGAA 
GTGTGTGATA ACAGCAGAAA GCAGATGGTG CGAAGCATGA GCAACAAGAG ACGATTCTCG 
TTTCGTATTC CTGAGGAGGA GAGACTGCAG CAGAGGAGAG AAATGTTGAA AGATCCTGAG 
AGGAGGAACA AGCTGATCTC AAATCCTACA AATTTTAACC ATGTGGCTCA TATGGGACCT 
GGAGATGGCA TGCAGATACT CAGAGACCTG CCAATGAATG TACGTGCTCA GGACAACCGT 
GCTGGATTCA GCGGCTCCGT CAGCATCCCC TCCATTACAA AAAACAGAGC AGAACCAGGG 
CGATCCATGA GCGCAAGCAG TGGCCTAGGA ATGCGGTCGT CCTCTCAGAA CGGCAGTGCT 
CTAAGGAGGG AGCTGTCCAG CGGCAGCTAC AGCTCTAAAC GGCAGACCAT GACTTCACCC 
TCCGAGAGTT CGCTGTCATC AGGAGGAGGG ATGGACGTGG GGGAAGCGCC GCTCTCTCAG 
TTTGACAGAG AGGACTCTGA TTCGCCGCGG CACTCCACTG CCTCCAACAG CTCCACCTTC 
AGCAGCCCGC CGAGCCCCGC CTCCCCACAC AAGACAAAGT CCCTCTCATT GGAGAGCACA 
GACCGCACCG CCTGGGACAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207996.1
CDS	1641..6944
Translation	MSVEVRLKKLEKLIMDGPSQSSGQCFSMETLLDILVCLYDECSSSPIRREKNVLEFLDWAKPFTSKVKQMRLHKEDFEILKVIGRGAFGEVAVVKVKNTDKVFAMKILNKWEMLKRAETACFREERDVLVNGDCQWITTLHYAFQDENNLYLVMDYYVGGDLLTLLSKFEDRLPEDMAKFYLAEMVLAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPLQVTDVSEDAKDLVRRLICSREHRLGQNGIEDFKQHPFFTGIDWDNIRTCEAPYIPEVSSPTDTSNFDVDDDCLKNCETLPPPSHTAFSGHHLPFVGFTYTSKCTLSDRGCLRESVGPAQVDAHVQRSLEESLASEAYERRIRRLEQEKTELTRKLQESTQTVQALQYPDSDAPVNANKEVEIRSLKSEIDILKKQIADSGQMEQQLENASTARRDLEDSSKVVRNLEKQLKSVTQEKDDLHKELLGLAEKVKAQGKELKDAHSQKKMAIKEFAELNEQLSDVRTQKQRLSRQFKDKEEEMEGVSQKLEALRLEIRKAERMRKELEAQAEEHAAEAQKERKVRERTEQYSKQLEEELEGMKLKQTGPPNGAASSDQQQELMRVRAELEKKSVQYEEEMCRRETLHSSEMKNLQKELRDAEGQHLTHQKEILVLKDKLEKTRRESQVEREEFVCEYKQKYEREKAHLTDENKKLSAEVEKLNGQFEQLSSSHRQLEEEMRELADKKESVAHWEAQITEIIQWVSDEKDARGYLQALATKMTEELEGLRNTSLGARGTDMPWKMRRFAKLDMSARLELQSALDTEIRAKQSIQDELNKVKATNIATECKLQESESRNQELLGEIERLRKETEELRLRRGVKHQDSQNSFLAFLNAPTSALDQFDRSPSFGPGSKTRRLDSADFTPSNTPSRDEDARSRRISRSRSPSTASDMEPIELIDHTSRSLQTPTGRSGYGSLGHSSPKPKAHQFSVKSFNVPTKCNQCTSLMVGLIRQGCTCEACSFSCHVTCADKAPSVCPVAPDQTRGKLGIDTQRGIGTAYEGHLRVPKPTGVKKGWQRVWAVVCDFKLFLYELGEGKGAQPGVVVNQVIDMRDEEFSASSVLESDVIHANRKDIPCIFRVTASQLSASSSQKTCILILADNEQERTKWVCALNELHRILRKNKLKERFVYVPKEAYDSTLPLIKTTQTAAIIDHERVALGNEEGLFVIHVTKDEIIRVGDVKKVHHVELMPTEHILAVISGRNRQVCLVSMAGLDGREVDKNKVADTKNCQLLVSGVVRNVTCFCLAIKRQISCFEINKSKSRHSHLVDIQAPGTVQWMALFNQQLCVGYPSGFMRYSLYGETPPASLLHPDDPTLAFIKTDNLDALCAVEISNKELLLCFSKIAVYVDTHGRRSRQQELMWPAAPTACCYNAPYLSVYSENAVDVFDVNSTEWIQTIPLKKVRPLNMDGSLNLLGLETVRLIYFRNKTAEGDELVVPEVCDNSRKQMVRSMSNKRRFSFRIPEEERLQQRREMLKDPERRNKLISNPTNFNHVAHMGPGDGMQILRDLPMNVRAQDNRAGFSGSVSIPSITKNRAEPGRSMSASSGLGMRSSSQNGSALRRELSSGSYSSKRQTMTSPSESSLSSGGGMDVGEAPLSQFDREDSDSPRHSTASNSSTFSSPPSPASPHKTKSLSLESTDRTAWDT

Target ORF information:

RefSeq Version	XM_017352507.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352507.2

ORF Insert Sequence:

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC 
TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG 
CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC 
CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT 
GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC 
TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA 
GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC 
GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA 
GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA 
CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT 
GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG 
ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC 
CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA 
GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG 
AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG 
AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC 
AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC 
AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT 
CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG 
CTCGAGGGCA TGAAGTTGAA GCAGACTGGG CCTCCTAACG GAGCTGCAAG CTCAGACCAG 
CAGCAGGAGC TGATGCGCGT TCGGGCTGAA CTGGAGAAGA AGAGCGTACA GTACGAGGAG 
GAGATGTGTC GCAGAGAGAC GCTCCACAGC AGTGAGATGA AGAACCTCCA AAAAGAGCTA 
CGGGATGCCG AGGGTCAACA CCTCACACAT CAGAAAGAGA TACTCGTGCT TAAAGACAAA 
CTGGAGAAAA CTCGCAGAGA AAGTCAAGTC GAGCGAGAAG AATTTGTGTG CGAGTACAAG 
CAGAAGTACG AGAGGGAAAA AGCCCATCTG ACAGACGAAA ACAAGAAGCT GAGTGCCGAA 
GTGGAGAAGT TGAACGGTCA GTTCGAGCAG CTGAGCAGCA GTCACCGGCA GCTGGAGGAG 
GAGATGAGAG AACTGGCTGA TAAGAAGGAG AGTGTTGCCC ACTGGGAGGC CCAGATCACC 
GAGATCATCC AGTGGGTGAG CGATGAGAAG GATGCCCGTG GTTACCTTCA AGCTCTGGCC 
ACCAAAATGA CAGAGGAGCT GGAAGGGCTC AGAAACACCA GTTTGGGAGC CAGAGGAACG 
GACATGCCGT GGAAGATGAG GCGTTTCGCC AAGCTGGACA TGTCTGCGCG TCTGGAGCTG 
CAGTCGGCGC TGGATACTGA GATCAGAGCC AAACAGTCCA TCCAAGACGA GCTTAACAAA 
GTCAAAGCAA CCAACATTGC CACAGAATGC AAATTGCAAG AATCCGAAAG TAGAAACCAG 
GAGCTGCTGG GAGAAATTGA GAGACTGAGG AAGGAGACAG AGGAGCTAAG GCTGAGGAGA 
GGCGTGAAGC ACCAGGATTC CCAAAATTCC TTTTTGGCTT TCCTCAATGC GCCCACTTCT 
GCACTGGATC AGTTTGATCG CTCACCCTCT TTTGGTCCCG GAAGCAAAAC CAGACGTCTT 
GACTCTGCAG ATTTCACCCC TTCCAACACT CCATCCAGAG ATGAAGACGC AAGATCTCGC 
CGTATTTCAC GCTCTCGCTC ACCCTCCACC GCCAGTGACA TGGAGCCTAT AGAGTTGATT 
GACCACACAT CACGTAGTCT TCAGACCCCA ACAGGCAGAT CAGGCTACGG GAGCTTGGGA 
CACTCATCAC CTAAGCCCAA GGCACACCAG TTTAGTGTAA AGTCCTTCAA TGTTCCCACT 
AAATGTAACC AGTGCACCTC TCTCATGGTG GGACTCATCC GTCAGGGCTG CACATGCGAA 
GCCTGCAGCT TCTCCTGTCA TGTCACTTGT GCTGATAAAG CCCCTTCTGT GTGCCCCGTC 
GCACCAGACC AGACCAGGGG CAAACTGGGC ATTGACACTC AGAGGGGCAT TGGGACTGCA 
TATGAGGGGC ATCTTAGGGT GCCGAAGCCC ACTGGGGTGA AGAAGGGTTG GCAGAGGGTC 
TGGGCAGTGG TTTGTGACTT CAAACTTTTT CTGTATGAGC TTGGAGAAGG GAAAGGTGCT 
CAGCCTGGTG TTGTAGTAAA CCAGGTCATT GACATGAGGG ATGAAGAGTT TTCCGCCAGT 
TCGGTTCTGG AGTCTGATGT CATCCATGCT AATAGGAAGG ATATACCCTG TATTTTTAGG 
GTAACAGCAT CTCAGCTGTC CGCCTCCTCC AGTCAGAAGA CGTGTATTCT AATTCTGGCT 
GACAACGAGC AAGAAAGGAC GAAGTGGGTG TGCGCTCTGA ATGAACTGCA TCGCATCCTG 
AGGAAAAACA AGCTGAAGGA GCGTTTCGTC TATGTGCCTA AAGAAGCCTA CGACAGCACA 
CTTCCTCTCA TCAAAACCAC CCAAACTGCT GCTATTATAG ATCATGAACG CGTTGCTTTG 
GGCAATGAGG AGGGCCTGTT TGTCATCCAC GTCACCAAAG ATGAAATCAT TCGTGTGGGT 
GATGTCAAGA AGGTGCATCA TGTAGAACTG ATGCCCACGG AGCACATACT GGCCGTCATC 
TCGGGCAGGA ACCGACAAGT ATGTCTGGTC TCCATGGCGG GTCTCGACGG GAGGGAGGTC 
GACAAGAATA AGGTGGCAGA TACTAAAAAC TGCCAGTTGC TGGTGTCCGG TGTGGTTCGC 
AATGTCACCT GCTTCTGCCT CGCCATTAAA CGGCAGATCT CATGCTTCGA AATTAACAAG 
AGCAAGTCAC GACACTCCCA TCTCGTGGAC ATACAAGCTC CAGGAACTGT CCAATGGATG 
GCGCTGTTCA ACCAGCAGTT GTGTGTTGGT TACCCATCAG GCTTCATGCG CTACAGTTTG 
TATGGAGAAA CACCTCCAGC CAGCTTACTT CATCCAGATG ACCCCACACT GGCATTTATT 
AAAACAGACA ACCTGGATGC CCTTTGTGCT GTGGAAATCT CCAATAAGGA ACTGCTGCTC 
TGCTTCAGTA AAATAGCAGT GTATGTTGAC ACACACGGCA GACGCTCGCG CCAGCAGGAG 
CTGATGTGGC CCGCGGCGCC CACCGCCTGC TGCTATAACG CCCCCTATCT GTCGGTCTAC 
AGTGAAAATG CAGTGGATGT GTTTGATGTG AACTCTACGG AATGGATTCA GACCATTCCT 
CTCAAAAAGG TCCGGCCATT AAACATGGAT GGCTCTTTGA ATCTCCTGGG CCTAGAAACG 
GTCAGACTCA TCTACTTCAG AAACAAGACC GCAGAGGGGG ATGAACTGGT GGTGCCTGAA 
GTGTGTGATA ACAGCAGAAA GCAGATGGTG CGAAGCATGA GCAACAAGAG ACGATTCTCG 
TTTCGTATTC CTGAGGAGGA GAGACTGCAG CAGAGGAGAG AAATGTTGAA AGATCCTGAG 
AGGAGGAACA AGCTGATCTC AAATCCTACA AATTTTAACC ATGTGGCTCA TATGGGACCT 
GGAGATGGCA TGCAGATACT CAGAGACCTG CCAATGAATG TACGTGCTCA GGACAACCGT 
GCTGGATTCA GCGGCTCCGT CAGCATCCCC TCCATTACAA AAAACAGAGC AGAACCAGGG 
CGATCCATGA GCGCAAGCAG TGGCCTAGGA ATGCGGTCGT CCTCTCAGAA CGGCAGTGCT 
CTAAGGAGGG AGCTGTCCAG CGGCAGCTAC AGCTCTAAAC GGCAGACCAT GACTTCACCC 
TCCGAGAGTT CGCTGTCATC AGGAGGAGGG ATGGACGTGG GGGAAGCGCC GCTCTCTCAG 
TTTGACAGAG AGGACTCTGA TTCGCCGCGG CACTCCACTG CCTCCAACAG CTCCACCTTC 
AGCAGCCCGC CGAGCCCCGC CTCCCCACAC AAGACAAAGT CCCTCTCATT GGAGAGCACA 
GACCGCACCG CCTGGGACAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-89p3.3 ( NM_001045094.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038559.1 Danio rerio CDC42 binding protein kinase alpha (DMPK-like) b (cdc42bpab), mRNA.

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CloneID	ODa33549
Clone ID Related Accession (Same CDS sequence)	NM_001045094.1
Accession Version	NM_001045094.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase MRCK alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX470246.11 and BX511007.16. On or before Jul 22, 2013 this sequence version replaced NM_001113343.1, XM_689268.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168447, SAMEA4476738 [ECO:0000350] ##Evidence-Data-END##

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC 
TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG 
CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC 
CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT 
GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC 
TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA 
GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC 
GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA 
GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA 
CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT 
GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG 
ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC 
CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA 
GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG 
AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG 
AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC 
AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC 
AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT 
CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG 
CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC 
CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG 
GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG 
CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC 
AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC 
AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC 
GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG 
GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC 
ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG 
GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA 
ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG 
CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC 
AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC 
CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG 
AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT 
TCTGCACTGG ATCAGTTTGA TTTGATTGAC CACACATCAC GTAGTCTTCA GACCCCAACA 
GGCAGATCAG GCTACGGGAG CTTGGGACAC TCATCACCTA AGCCCAAGGC ACACCAGTTT 
AGTGTAAAGT CCTTCAATGT TCCCACTAAA TGTAACCAGT GCACCTCTCT CATGGTGGGA 
CTCATCCGTC AGGGCTGCAC ATGCGAAGCC TGCAGCTTCT CCTGTCATGT CACTTGTGCT 
GATAAAGCCC CTTCTGTGTG CCCCGTCGCA CCAGACCAGA CCAGGGGCAA ACTGGGCATT 
GACACTCAGA GGGGCATTGG GACTGCATAT GAGGGGCATC TTAGGGTGCC GAAGCCCACT 
GGGGTGAAGA AGGGTTGGCA GAGGGTCTGG GCAGTGGTTT GTGACTTCAA ACTTTTTCTG 
TATGAGCTTG GAGAAGGGAA AGGTGCTCAG CCTGGTGTTG TAGTAAACCA GGTCATTGAC 
ATGAGGGATG AAGAGTTTTC CGCCAGTTCG GTTCTGGAGT CTGATGTCAT CCATGCTAAT 
AGGAAGGATA TACCCTGTAT TTTTAGGGTA ACAGCATCTC AGCTGTCCGC CTCCTCCAGT 
CAGAAGACGT GTATTCTAAT TCTGGCTGAC AACGAGCAAG AAAGGACGAA GTGGGTGTGC 
GCTCTGAATG AACTGCATCG CATCCTGAGG AAAAACAAGC TGAAGGAGCG TTTCGTCTAT 
GTGCCTAAAG AAGCCTACGA CAGCACACTT CCTCTCATCA AAACCACCCA AACTGCTGCT 
ATTATAGATC ATGAACGCGT TGCTTTGGGC AATGAGGAGG GCCTGTTTGT CATCCACGTC 
ACCAAAGATG AAATCATTCG TGTGGGTGAT GTCAAGAAGG TGCATCATGT AGAACTGATG 
CCCACGGAGC ACATACTGGC CGTCATCTCG GGCAGGAACC GACAAGTATG TCTGGTCTCC 
ATGGCGGGTC TCGACGGGAG GGAGGTCGAC AAGAATAAGG TGGCAGATAC TAAAAACTGC 
CAGTTGCTGG TGTCCGGTGT GGTTCGCAAT GTCACCTGCT TCTGCCTCGC CATTAAACGG 
CAGATCTCAT GCTTCGAAAT TAACAAGAGC AAGTCACGAC ACTCCCATCT CGTGGACATA 
CAAGCTCCAG GAACTGTCCA ATGGATGGCG CTGTTCAACC AGCAGTTGTG TGTTGGTTAC 
CCATCAGGCT TCATGCGCTA CAGTTTGTAT GGAGAAACAC CTCCAGCCAG CTTACTTCAT 
CCAGATGACC CCACACTGGC ATTTATTAAA ACAGACAACC TGGATGCCCT TTGTGCTGTG 
GAAATCTCCA ATAAGGAACT GCTGCTCTGC TTCAGTAAAA TAGCAGTGTA TGTTGACACA 
CACGGCAGAC GCTCGCGCCA GCAGGAGCTG ATGTGGCCCG CGGCGCCCAC CGCCTGCTGC 
TATAACGCCC CCTATCTGTC GGTCTACAGT GAAAATGCAG TGGATGTGTT TGATGTGAAC 
TCTACGGAAT GGATTCAGAC CATTCCTCTC AAAAAGGTCC GGCCATTAAA CATGGATGGC 
TCTTTGAATC TCCTGGGCCT AGAAACGGTC AGACTCATCT ACTTCAGAAA CAAGACCGCA 
GAGGGGGATG AACTGGTGGT GCCTGAAGTG TGTGATAACA GCAGAAAGCA GATGGTGCGA 
AGCATGAGCA ACAAGAGACG ATTCTCGTTT CGTATTCCTG AGGAGGAGAG ACTGCAGCAG 
AGGAGAGAAA TGTTGAAAGA TCCTGAGAGG AGGAACAAGC TGATCTCAAA TCCTACAAAT 
TTTAACCATG TGGCTCATAT GGGACCTGGA GATGGCATGC AGATACTCAG AGACCTGCCA 
ATGAATGTAC GTGCTCAGGA CAACCGTGCT GGATTCAGCG GCTCCGTCAG CATCCCCTCC 
ATTACAAAAA ACAGAGCAGA ACCAGGGCGA TCCATGAGCG CAAGCAGTGG CCTAGGAATG 
CGGTCGTCCT CTCAGAACGG CAGTGCTCTA AGGAGGGAGC TGTCCAGCGG CAGCTACAGC 
TCTAAACGGC AGACCATGAC TTCACCCTCC GAGAGTTCGC TGTCATCAGG AGGAGGGATG 
GACGTGGGGG AAGCGCCGCT CTCTCAGTTT GACAGAGAGG ACTCTGATTC GCCGCGGCAC 
TCCACTGCCT CCAACAGCTC CACCTTCAGC AGCCCGCCGA GCCCCGCCTC CCCACACAAG 
ACAAAGTCCC TCTCATTGGA GAGCACAGAC CGCACCGCCT GGGACACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038559.1
CDS	1..5151
Translation	MSVEVRLKKLEKLIMDGPSQSSGQCFSMETLLDILVCLYDECSSSPIRREKNVLEFLDWAKPFTSKVKQMRLHKEDFEILKVIGRGAFGEVAVVKVKNTDKVFAMKILNKWEMLKRAETACFREERDVLVNGDCQWITTLHYAFQDENNLYLVMDYYVGGDLLTLLSKFEDRLPEDMAKFYLAEMVLAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPLQVTDVSEDAKDLVRRLICSREHRLGQNGIEDFKQHPFFTGIDWDNIRTCEAPYIPEVSSPTDTSNFDVDDDCLKNCETLPPPSHTAFSGHHLPFVGFTYTSKCTLSDRGCLRESVGPAQVDAHVQRSLEESLASEAYERRIRRLEQEKTELTRKLQESTQTVQALQYPDSDAPVNANKEVEIRSLKSEIDILKKQIADSGQMEQQLENASTARRDLEDSSKVVRNLEKQLKSVTQEKDDLHKELLGLAEKVKAQGKELKDAHSQKKMAIKEFAELNEQLSDVRTQKQRLSRQFKDKEEEMEGVSQKLEALRLEIRKAERMRKELEAQAEEHAAEAQKERKVRERTEQYSKQLEEELEGMKQLKQTGPPNGAASSDQQQELMRVRAELEKKSVQYEEEMCRRETLHSSEMKNLQKELRDAEGQHLTHQKEILVLKDKLEKTRRESQVEREEFVCEYKQKYEREKAHLTDENKKLSAEVEKLNGQFEQLSSSHRQLEEEMRELADKKESVAHWEAQITEIIQWVSDEKDARGYLQALATKMTEELEGLRNTSLGARGTDMPWKMRRFAKLDMSARLELQSALDTEIRAKQSIQDELNKVKATNIATECKLQESESRNQELLGEIERLRKETEELRLRRGVKHQDSQNSFLAFLNAPTSALDQFDLIDHTSRSLQTPTGRSGYGSLGHSSPKPKAHQFSVKSFNVPTKCNQCTSLMVGLIRQGCTCEACSFSCHVTCADKAPSVCPVAPDQTRGKLGIDTQRGIGTAYEGHLRVPKPTGVKKGWQRVWAVVCDFKLFLYELGEGKGAQPGVVVNQVIDMRDEEFSASSVLESDVIHANRKDIPCIFRVTASQLSASSSQKTCILILADNEQERTKWVCALNELHRILRKNKLKERFVYVPKEAYDSTLPLIKTTQTAAIIDHERVALGNEEGLFVIHVTKDEIIRVGDVKKVHHVELMPTEHILAVISGRNRQVCLVSMAGLDGREVDKNKVADTKNCQLLVSGVVRNVTCFCLAIKRQISCFEINKSKSRHSHLVDIQAPGTVQWMALFNQQLCVGYPSGFMRYSLYGETPPASLLHPDDPTLAFIKTDNLDALCAVEISNKELLLCFSKIAVYVDTHGRRSRQQELMWPAAPTACCYNAPYLSVYSENAVDVFDVNSTEWIQTIPLKKVRPLNMDGSLNLLGLETVRLIYFRNKTAEGDELVVPEVCDNSRKQMVRSMSNKRRFSFRIPEEERLQQRREMLKDPERRNKLISNPTNFNHVAHMGPGDGMQILRDLPMNVRAQDNRAGFSGSVSIPSITKNRAEPGRSMSASSGLGMRSSSQNGSALRRELSSGSYSSKRQTMTSPSESSLSSGGGMDVGEAPLSQFDREDSDSPRHSTASNSSTFSSPPSPASPHKTKSLSLESTDRTAWDT

Target ORF information:

RefSeq Version	NM_001045094.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio CDC42 binding protein kinase alpha (DMPK-like) b (cdc42bpab), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045094.1

ORF Insert Sequence:

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC 
TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG 
CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC 
CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT 
GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC 
TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA 
GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC 
GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA 
GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA 
CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT 
GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG 
ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC 
CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA 
GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG 
AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG 
AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC 
AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC 
AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT 
CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG 
CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC 
CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG 
GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG 
CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC 
AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC 
AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC 
GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG 
GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC 
ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG 
GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA 
ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG 
CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC 
AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC 
CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG 
AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT 
TCTGCACTGG ATCAGTTTGA TTTGATTGAC CACACATCAC GTAGTCTTCA GACCCCAACA 
GGCAGATCAG GCTACGGGAG CTTGGGACAC TCATCACCTA AGCCCAAGGC ACACCAGTTT 
AGTGTAAAGT CCTTCAATGT TCCCACTAAA TGTAACCAGT GCACCTCTCT CATGGTGGGA 
CTCATCCGTC AGGGCTGCAC ATGCGAAGCC TGCAGCTTCT CCTGTCATGT CACTTGTGCT 
GATAAAGCCC CTTCTGTGTG CCCCGTCGCA CCAGACCAGA CCAGGGGCAA ACTGGGCATT 
GACACTCAGA GGGGCATTGG GACTGCATAT GAGGGGCATC TTAGGGTGCC GAAGCCCACT 
GGGGTGAAGA AGGGTTGGCA GAGGGTCTGG GCAGTGGTTT GTGACTTCAA ACTTTTTCTG 
TATGAGCTTG GAGAAGGGAA AGGTGCTCAG CCTGGTGTTG TAGTAAACCA GGTCATTGAC 
ATGAGGGATG AAGAGTTTTC CGCCAGTTCG GTTCTGGAGT CTGATGTCAT CCATGCTAAT 
AGGAAGGATA TACCCTGTAT TTTTAGGGTA ACAGCATCTC AGCTGTCCGC CTCCTCCAGT 
CAGAAGACGT GTATTCTAAT TCTGGCTGAC AACGAGCAAG AAAGGACGAA GTGGGTGTGC 
GCTCTGAATG AACTGCATCG CATCCTGAGG AAAAACAAGC TGAAGGAGCG TTTCGTCTAT 
GTGCCTAAAG AAGCCTACGA CAGCACACTT CCTCTCATCA AAACCACCCA AACTGCTGCT 
ATTATAGATC ATGAACGCGT TGCTTTGGGC AATGAGGAGG GCCTGTTTGT CATCCACGTC 
ACCAAAGATG AAATCATTCG TGTGGGTGAT GTCAAGAAGG TGCATCATGT AGAACTGATG 
CCCACGGAGC ACATACTGGC CGTCATCTCG GGCAGGAACC GACAAGTATG TCTGGTCTCC 
ATGGCGGGTC TCGACGGGAG GGAGGTCGAC AAGAATAAGG TGGCAGATAC TAAAAACTGC 
CAGTTGCTGG TGTCCGGTGT GGTTCGCAAT GTCACCTGCT TCTGCCTCGC CATTAAACGG 
CAGATCTCAT GCTTCGAAAT TAACAAGAGC AAGTCACGAC ACTCCCATCT CGTGGACATA 
CAAGCTCCAG GAACTGTCCA ATGGATGGCG CTGTTCAACC AGCAGTTGTG TGTTGGTTAC 
CCATCAGGCT TCATGCGCTA CAGTTTGTAT GGAGAAACAC CTCCAGCCAG CTTACTTCAT 
CCAGATGACC CCACACTGGC ATTTATTAAA ACAGACAACC TGGATGCCCT TTGTGCTGTG 
GAAATCTCCA ATAAGGAACT GCTGCTCTGC TTCAGTAAAA TAGCAGTGTA TGTTGACACA 
CACGGCAGAC GCTCGCGCCA GCAGGAGCTG ATGTGGCCCG CGGCGCCCAC CGCCTGCTGC 
TATAACGCCC CCTATCTGTC GGTCTACAGT GAAAATGCAG TGGATGTGTT TGATGTGAAC 
TCTACGGAAT GGATTCAGAC CATTCCTCTC AAAAAGGTCC GGCCATTAAA CATGGATGGC 
TCTTTGAATC TCCTGGGCCT AGAAACGGTC AGACTCATCT ACTTCAGAAA CAAGACCGCA 
GAGGGGGATG AACTGGTGGT GCCTGAAGTG TGTGATAACA GCAGAAAGCA GATGGTGCGA 
AGCATGAGCA ACAAGAGACG ATTCTCGTTT CGTATTCCTG AGGAGGAGAG ACTGCAGCAG 
AGGAGAGAAA TGTTGAAAGA TCCTGAGAGG AGGAACAAGC TGATCTCAAA TCCTACAAAT 
TTTAACCATG TGGCTCATAT GGGACCTGGA GATGGCATGC AGATACTCAG AGACCTGCCA 
ATGAATGTAC GTGCTCAGGA CAACCGTGCT GGATTCAGCG GCTCCGTCAG CATCCCCTCC 
ATTACAAAAA ACAGAGCAGA ACCAGGGCGA TCCATGAGCG CAAGCAGTGG CCTAGGAATG 
CGGTCGTCCT CTCAGAACGG CAGTGCTCTA AGGAGGGAGC TGTCCAGCGG CAGCTACAGC 
TCTAAACGGC AGACCATGAC TTCACCCTCC GAGAGTTCGC TGTCATCAGG AGGAGGGATG 
GACGTGGGGG AAGCGCCGCT CTCTCAGTTT GACAGAGAGG ACTCTGATTC GCCGCGGCAC 
TCCACTGCCT CCAACAGCTC CACCTTCAGC AGCCCGCCGA GCCCCGCCTC CCCACACAAG 
ACAAAGTCCC TCTCATTGGA GAGCACAGAC CGCACCGCCT GGGACACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-89p3.3 ( XM_005160749.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160806.1 Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X4, mRNA.

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CloneID	ODa25024
Clone ID Related Accession (Same CDS sequence)	XM_005160749.4 , XM_005160749.3
Accession Version	XM_005160749.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase MRCK alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160749.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC 
TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG 
CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC 
CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT 
GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC 
TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA 
GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC 
GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA 
GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA 
CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT 
GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG 
ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC 
CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA 
GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG 
AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG 
AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC 
AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC 
AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT 
CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG 
CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC 
CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG 
GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG 
CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC 
AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC 
AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC 
GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG 
GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC 
ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG 
GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA 
ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG 
CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC 
AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC 
CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG 
AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT 
TCTGCACTGG ATCAGTTTGA TCGCTCACCC TCTTTTGGTC CCGGAAGCAA AACCAGACGT 
CTTGACTCTG CAGATTTCAC CCCTTCCAAC ACTCCATCCA GAGATGAAGA CGCAAGATCT 
CGCCGTATTT CACGCTCTCG CTCACCCTCC ACCGCCAGTG ACATGGAGCC TATAGAGTTG 
ATTGACCACA CATCACGTAG TCTTCAGACC CCAACAGGCA GATCAGGCTA CGGGAGCTTG 
GGACACTCAT CACCTAAGCC CAAGGCACAC CAGTTTAGTG TAAAGTCCTT CAATGTTCCC 
ACTAAATGTA ACCAGTGCAC CTCTCTCATG GTGGGACTCA TCCGTCAGGG CTGCACATGC 
GAAGCCTGCA GCTTCTCCTG TCATGTCACT TGTGCTGATA AAGCCCCTTC TGTGTGCCCC 
GTCGCACCAG ACCAGACCAG GGGCAAACTG GGCATTGACA CTCAGAGGGG CATTGGGACT 
GCATATGAGG GGCATCTTAG GGTGCCGAAG CCCACTGGGG TGAAGAAGGG TTGGCAGAGG 
GTCTGGGCAG TGGTTTGTGA CTTCAAACTT TTTCTGTATG AGCTTGGAGA AGGGAAAGGT 
GCTCAGCCTG GTGTTGTAGT AAACCAGGTC ATTGACATGA GGGATGAAGA GTTTTCCGCC 
AGTTCGGTTC TGGAGTCTGA TGTCATCCAT GCTAATAGGA AGGATATACC CTGTATTTTT 
AGGGTAACAG CATCTCAGCT GTCCGCCTCC TCCAGTCAGA AGACGTGTAT TCTAATTCTG 
GCTGACAACG AGCAAGAAAG GACGAAGTGG GTGTGCGCTC TGAATGAACT GCATCGCATC 
CTGAGGAAAA ACAAGCTGAA GGAGCGTTTC GTCTATGTGC CTAAAGAAGC CTACGACAGC 
ACACTTCCTC TCATCAAAAC CACCCAAACT GCTGCTATTA TAGATCATGA ACGCGTTGCT 
TTGGGCAATG AGGAGGGCCT GTTTGTCATC CACGTCACCA AAGATGAAAT CATTCGTGTG 
GGTGATGTCA AGAAGGTGCA TCATGTAGAA CTGATGCCCA CGGAGCACAT ACTGGCCGTC 
ATCTCGGGCA GGAACCGACA AGTATGTCTG GTCTCCATGG CGGGTCTCGA CGGGAGGGAG 
GTCGACAAGA ATAAGGTGGC AGATACTAAA AACTGCCAGT TGCTGGTGTC CGGTGTGGTT 
CGCAATGTCA CCTGCTTCTG CCTCGCCATT AAACGGCAGA TCTCATGCTT CGAAATTAAC 
AAGAGCAAGT CACGACACTC CCATCTCGTG GACATACAAG CTCCAGGAAC TGTCCAATGG 
ATGGCGCTGT TCAACCAGCA GTTGTGTGTT GGTTACCCAT CAGGCTTCAT GCGCTACAGT 
TTGTATGGAG AAACACCTCC AGCCAGCTTA CTTCATCCAG ATGACCCCAC ACTGGCATTT 
ATTAAAACAG ACAACCTGGA TGCCCTTTGT GCTGTGGAAA TCTCCAATAA GGAACTGCTG 
CTCTGCTTCA GTAAAATAGC AGTGTATGTT GACACACACG GCAGACGCTC GCGCCAGCAG 
GAGCTGATGT GGCCCGCGGC GCCCACCGCC TGCTGCTATA ACGCCCCCTA TCTGTCGGTC 
TACAGTGAAA ATGCAGTGGA TGTGTTTGAT GTGAACTCTA CGGAATGGAT TCAGACCATT 
CCTCTCAAAA AGGTCCGGCC ATTAAACATG GATGGCTCTT TGAATCTCCT GGGCCTAGAA 
ACGGTCAGAC TCATCTACTT CAGAAACAAG ACCGCAGAGG GGGATGAACT GGTGGTGCCT 
GAAGTGTGTG ATAACAGCAG AAAGCAGATG GTGCGAAGCA TGAGCAACAA GAGACGATTC 
TCGTTTCGTA TTCCTGAGGA GGAGAGACTG CAGCAGAGGA GAGAAATGTT GAAAGATCCT 
GAGAGGAGGA ACAAGCTGAT CTCAAATCCT ACAAATTTTA ACCATGTGGC TCATATGGGA 
CCTGGAGATG GCATGCAGAT ACTCAGAGAC CTGCCAATGA ATGTACGTGC TCAGGACAAC 
CGTGCTGGAT TCAGCGGCTC CGTCAGCATC CCCTCCATTA CAAAAAACAG AGCAGAACCA 
GGGCGATCCA TGAGCGCAAG CAGTGGCCTA GGAATGCGGT CGTCCTCTCA GAACGGCAGT 
GCTCTAAGGA GGGAGCTGTC CAGCGGCAGC TACAGCTCTA AACGGCAGAC CATGACTTCA 
CCCTCCGAGA GTTCGCTGTC ATCAGGAGGA GGGATGGACG TGGGGGAAGC GCCGCTCTCT 
CAGTTTGACA GAGAGTGGCA GGCAGTATCG CCCTCGGAGA AGACCCCTGA TCTGAAAAGG 
GCTTTAAGGA CCCTGCTTAG TAAGATGAAG GACTCTGATT CGCCGCGGCA CTCCACTGCC 
TCCAACAGCT CCACCTTCAG CAGCCCGCCG AGCCCCGCCT CCCCACACAA GACAAAGTCC 
CTCTCATTGG AGAGCACAGA CCGCACCGCC TGGGACACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160806.1
CDS	1641..7022
Translation	MSVEVRLKKLEKLIMDGPSQSSGQCFSMETLLDILVCLYDECSSSPIRREKNVLEFLDWAKPFTSKVKQMRLHKEDFEILKVIGRGAFGEVAVVKVKNTDKVFAMKILNKWEMLKRAETACFREERDVLVNGDCQWITTLHYAFQDENNLYLVMDYYVGGDLLTLLSKFEDRLPEDMAKFYLAEMVLAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPLQVTDVSEDAKDLVRRLICSREHRLGQNGIEDFKQHPFFTGIDWDNIRTCEAPYIPEVSSPTDTSNFDVDDDCLKNCETLPPPSHTAFSGHHLPFVGFTYTSKCTLSDRGCLRESVGPAQVDAHVQRSLEESLASEAYERRIRRLEQEKTELTRKLQESTQTVQALQYPDSDAPVNANKEVEIRSLKSEIDILKKQIADSGQMEQQLENASTARRDLEDSSKVVRNLEKQLKSVTQEKDDLHKELLGLAEKVKAQGKELKDAHSQKKMAIKEFAELNEQLSDVRTQKQRLSRQFKDKEEEMEGVSQKLEALRLEIRKAERMRKELEAQAEEHAAEAQKERKVRERTEQYSKQLEEELEGMKQLKQTGPPNGAASSDQQQELMRVRAELEKKSVQYEEEMCRRETLHSSEMKNLQKELRDAEGQHLTHQKEILVLKDKLEKTRRESQVEREEFVCEYKQKYEREKAHLTDENKKLSAEVEKLNGQFEQLSSSHRQLEEEMRELADKKESVAHWEAQITEIIQWVSDEKDARGYLQALATKMTEELEGLRNTSLGARGTDMPWKMRRFAKLDMSARLELQSALDTEIRAKQSIQDELNKVKATNIATECKLQESESRNQELLGEIERLRKETEELRLRRGVKHQDSQNSFLAFLNAPTSALDQFDRSPSFGPGSKTRRLDSADFTPSNTPSRDEDARSRRISRSRSPSTASDMEPIELIDHTSRSLQTPTGRSGYGSLGHSSPKPKAHQFSVKSFNVPTKCNQCTSLMVGLIRQGCTCEACSFSCHVTCADKAPSVCPVAPDQTRGKLGIDTQRGIGTAYEGHLRVPKPTGVKKGWQRVWAVVCDFKLFLYELGEGKGAQPGVVVNQVIDMRDEEFSASSVLESDVIHANRKDIPCIFRVTASQLSASSSQKTCILILADNEQERTKWVCALNELHRILRKNKLKERFVYVPKEAYDSTLPLIKTTQTAAIIDHERVALGNEEGLFVIHVTKDEIIRVGDVKKVHHVELMPTEHILAVISGRNRQVCLVSMAGLDGREVDKNKVADTKNCQLLVSGVVRNVTCFCLAIKRQISCFEINKSKSRHSHLVDIQAPGTVQWMALFNQQLCVGYPSGFMRYSLYGETPPASLLHPDDPTLAFIKTDNLDALCAVEISNKELLLCFSKIAVYVDTHGRRSRQQELMWPAAPTACCYNAPYLSVYSENAVDVFDVNSTEWIQTIPLKKVRPLNMDGSLNLLGLETVRLIYFRNKTAEGDELVVPEVCDNSRKQMVRSMSNKRRFSFRIPEEERLQQRREMLKDPERRNKLISNPTNFNHVAHMGPGDGMQILRDLPMNVRAQDNRAGFSGSVSIPSITKNRAEPGRSMSASSGLGMRSSSQNGSALRRELSSGSYSSKRQTMTSPSESSLSSGGGMDVGEAPLSQFDREWQAVSPSEKTPDLKRALRTLLSKMKDSDSPRHSTASNSSTFSSPPSPASPHKTKSLSLESTDRTAWDT

Target ORF information:

RefSeq Version	XM_005160749.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160749.4

ORF Insert Sequence:

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC 
TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG 
CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC 
CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT 
GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC 
TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA 
GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC 
GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA 
GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA 
CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT 
GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG 
ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC 
CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA 
GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG 
AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG 
AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC 
AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC 
AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT 
CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG 
CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC 
CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG 
GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG 
CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC 
AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC 
AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC 
GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG 
GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC 
ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG 
GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA 
ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG 
CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC 
AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC 
CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG 
AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT 
TCTGCACTGG ATCAGTTTGA TCGCTCACCC TCTTTTGGTC CCGGAAGCAA AACCAGACGT 
CTTGACTCTG CAGATTTCAC CCCTTCCAAC ACTCCATCCA GAGATGAAGA CGCAAGATCT 
CGCCGTATTT CACGCTCTCG CTCACCCTCC ACCGCCAGTG ACATGGAGCC TATAGAGTTG 
ATTGACCACA CATCACGTAG TCTTCAGACC CCAACAGGCA GATCAGGCTA CGGGAGCTTG 
GGACACTCAT CACCTAAGCC CAAGGCACAC CAGTTTAGTG TAAAGTCCTT CAATGTTCCC 
ACTAAATGTA ACCAGTGCAC CTCTCTCATG GTGGGACTCA TCCGTCAGGG CTGCACATGC 
GAAGCCTGCA GCTTCTCCTG TCATGTCACT TGTGCTGATA AAGCCCCTTC TGTGTGCCCC 
GTCGCACCAG ACCAGACCAG GGGCAAACTG GGCATTGACA CTCAGAGGGG CATTGGGACT 
GCATATGAGG GGCATCTTAG GGTGCCGAAG CCCACTGGGG TGAAGAAGGG TTGGCAGAGG 
GTCTGGGCAG TGGTTTGTGA CTTCAAACTT TTTCTGTATG AGCTTGGAGA AGGGAAAGGT 
GCTCAGCCTG GTGTTGTAGT AAACCAGGTC ATTGACATGA GGGATGAAGA GTTTTCCGCC 
AGTTCGGTTC TGGAGTCTGA TGTCATCCAT GCTAATAGGA AGGATATACC CTGTATTTTT 
AGGGTAACAG CATCTCAGCT GTCCGCCTCC TCCAGTCAGA AGACGTGTAT TCTAATTCTG 
GCTGACAACG AGCAAGAAAG GACGAAGTGG GTGTGCGCTC TGAATGAACT GCATCGCATC 
CTGAGGAAAA ACAAGCTGAA GGAGCGTTTC GTCTATGTGC CTAAAGAAGC CTACGACAGC 
ACACTTCCTC TCATCAAAAC CACCCAAACT GCTGCTATTA TAGATCATGA ACGCGTTGCT 
TTGGGCAATG AGGAGGGCCT GTTTGTCATC CACGTCACCA AAGATGAAAT CATTCGTGTG 
GGTGATGTCA AGAAGGTGCA TCATGTAGAA CTGATGCCCA CGGAGCACAT ACTGGCCGTC 
ATCTCGGGCA GGAACCGACA AGTATGTCTG GTCTCCATGG CGGGTCTCGA CGGGAGGGAG 
GTCGACAAGA ATAAGGTGGC AGATACTAAA AACTGCCAGT TGCTGGTGTC CGGTGTGGTT 
CGCAATGTCA CCTGCTTCTG CCTCGCCATT AAACGGCAGA TCTCATGCTT CGAAATTAAC 
AAGAGCAAGT CACGACACTC CCATCTCGTG GACATACAAG CTCCAGGAAC TGTCCAATGG 
ATGGCGCTGT TCAACCAGCA GTTGTGTGTT GGTTACCCAT CAGGCTTCAT GCGCTACAGT 
TTGTATGGAG AAACACCTCC AGCCAGCTTA CTTCATCCAG ATGACCCCAC ACTGGCATTT 
ATTAAAACAG ACAACCTGGA TGCCCTTTGT GCTGTGGAAA TCTCCAATAA GGAACTGCTG 
CTCTGCTTCA GTAAAATAGC AGTGTATGTT GACACACACG GCAGACGCTC GCGCCAGCAG 
GAGCTGATGT GGCCCGCGGC GCCCACCGCC TGCTGCTATA ACGCCCCCTA TCTGTCGGTC 
TACAGTGAAA ATGCAGTGGA TGTGTTTGAT GTGAACTCTA CGGAATGGAT TCAGACCATT 
CCTCTCAAAA AGGTCCGGCC ATTAAACATG GATGGCTCTT TGAATCTCCT GGGCCTAGAA 
ACGGTCAGAC TCATCTACTT CAGAAACAAG ACCGCAGAGG GGGATGAACT GGTGGTGCCT 
GAAGTGTGTG ATAACAGCAG AAAGCAGATG GTGCGAAGCA TGAGCAACAA GAGACGATTC 
TCGTTTCGTA TTCCTGAGGA GGAGAGACTG CAGCAGAGGA GAGAAATGTT GAAAGATCCT 
GAGAGGAGGA ACAAGCTGAT CTCAAATCCT ACAAATTTTA ACCATGTGGC TCATATGGGA 
CCTGGAGATG GCATGCAGAT ACTCAGAGAC CTGCCAATGA ATGTACGTGC TCAGGACAAC 
CGTGCTGGAT TCAGCGGCTC CGTCAGCATC CCCTCCATTA CAAAAAACAG AGCAGAACCA 
GGGCGATCCA TGAGCGCAAG CAGTGGCCTA GGAATGCGGT CGTCCTCTCA GAACGGCAGT 
GCTCTAAGGA GGGAGCTGTC CAGCGGCAGC TACAGCTCTA AACGGCAGAC CATGACTTCA 
CCCTCCGAGA GTTCGCTGTC ATCAGGAGGA GGGATGGACG TGGGGGAAGC GCCGCTCTCT 
CAGTTTGACA GAGAGTGGCA GGCAGTATCG CCCTCGGAGA AGACCCCTGA TCTGAAAAGG 
GCTTTAAGGA CCCTGCTTAG TAAGATGAAG GACTCTGATT CGCCGCGGCA CTCCACTGCC 
TCCAACAGCT CCACCTTCAG CAGCCCGCCG AGCCCCGCCT CCCCACACAA GACAAAGTCC 
CTCTCATTGG AGAGCACAGA CCGCACCGCC TGGGACACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-89p3.3 ( XM_005160749.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160806.1 Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X1, mRNA.

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CloneID	ODa25024
Clone ID Related Accession (Same CDS sequence)	XM_005160749.4 , XM_005160749.3
Accession Version	XM_005160749.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase MRCK alpha isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160749.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC 
TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG 
CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC 
CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT 
GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC 
TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA 
GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC 
GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA 
GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA 
CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT 
GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG 
ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC 
CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA 
GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG 
AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG 
AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC 
AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC 
AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT 
CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG 
CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC 
CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG 
GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG 
CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC 
AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC 
AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC 
GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG 
GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC 
ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG 
GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA 
ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG 
CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC 
AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC 
CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG 
AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT 
TCTGCACTGG ATCAGTTTGA TCGCTCACCC TCTTTTGGTC CCGGAAGCAA AACCAGACGT 
CTTGACTCTG CAGATTTCAC CCCTTCCAAC ACTCCATCCA GAGATGAAGA CGCAAGATCT 
CGCCGTATTT CACGCTCTCG CTCACCCTCC ACCGCCAGTG ACATGGAGCC TATAGAGTTG 
ATTGACCACA CATCACGTAG TCTTCAGACC CCAACAGGCA GATCAGGCTA CGGGAGCTTG 
GGACACTCAT CACCTAAGCC CAAGGCACAC CAGTTTAGTG TAAAGTCCTT CAATGTTCCC 
ACTAAATGTA ACCAGTGCAC CTCTCTCATG GTGGGACTCA TCCGTCAGGG CTGCACATGC 
GAAGCCTGCA GCTTCTCCTG TCATGTCACT TGTGCTGATA AAGCCCCTTC TGTGTGCCCC 
GTCGCACCAG ACCAGACCAG GGGCAAACTG GGCATTGACA CTCAGAGGGG CATTGGGACT 
GCATATGAGG GGCATCTTAG GGTGCCGAAG CCCACTGGGG TGAAGAAGGG TTGGCAGAGG 
GTCTGGGCAG TGGTTTGTGA CTTCAAACTT TTTCTGTATG AGCTTGGAGA AGGGAAAGGT 
GCTCAGCCTG GTGTTGTAGT AAACCAGGTC ATTGACATGA GGGATGAAGA GTTTTCCGCC 
AGTTCGGTTC TGGAGTCTGA TGTCATCCAT GCTAATAGGA AGGATATACC CTGTATTTTT 
AGGGTAACAG CATCTCAGCT GTCCGCCTCC TCCAGTCAGA AGACGTGTAT TCTAATTCTG 
GCTGACAACG AGCAAGAAAG GACGAAGTGG GTGTGCGCTC TGAATGAACT GCATCGCATC 
CTGAGGAAAA ACAAGCTGAA GGAGCGTTTC GTCTATGTGC CTAAAGAAGC CTACGACAGC 
ACACTTCCTC TCATCAAAAC CACCCAAACT GCTGCTATTA TAGATCATGA ACGCGTTGCT 
TTGGGCAATG AGGAGGGCCT GTTTGTCATC CACGTCACCA AAGATGAAAT CATTCGTGTG 
GGTGATGTCA AGAAGGTGCA TCATGTAGAA CTGATGCCCA CGGAGCACAT ACTGGCCGTC 
ATCTCGGGCA GGAACCGACA AGTATGTCTG GTCTCCATGG CGGGTCTCGA CGGGAGGGAG 
GTCGACAAGA ATAAGGTGGC AGATACTAAA AACTGCCAGT TGCTGGTGTC CGGTGTGGTT 
CGCAATGTCA CCTGCTTCTG CCTCGCCATT AAACGGCAGA TCTCATGCTT CGAAATTAAC 
AAGAGCAAGT CACGACACTC CCATCTCGTG GACATACAAG CTCCAGGAAC TGTCCAATGG 
ATGGCGCTGT TCAACCAGCA GTTGTGTGTT GGTTACCCAT CAGGCTTCAT GCGCTACAGT 
TTGTATGGAG AAACACCTCC AGCCAGCTTA CTTCATCCAG ATGACCCCAC ACTGGCATTT 
ATTAAAACAG ACAACCTGGA TGCCCTTTGT GCTGTGGAAA TCTCCAATAA GGAACTGCTG 
CTCTGCTTCA GTAAAATAGC AGTGTATGTT GACACACACG GCAGACGCTC GCGCCAGCAG 
GAGCTGATGT GGCCCGCGGC GCCCACCGCC TGCTGCTATA ACGCCCCCTA TCTGTCGGTC 
TACAGTGAAA ATGCAGTGGA TGTGTTTGAT GTGAACTCTA CGGAATGGAT TCAGACCATT 
CCTCTCAAAA AGGTCCGGCC ATTAAACATG GATGGCTCTT TGAATCTCCT GGGCCTAGAA 
ACGGTCAGAC TCATCTACTT CAGAAACAAG ACCGCAGAGG GGGATGAACT GGTGGTGCCT 
GAAGTGTGTG ATAACAGCAG AAAGCAGATG GTGCGAAGCA TGAGCAACAA GAGACGATTC 
TCGTTTCGTA TTCCTGAGGA GGAGAGACTG CAGCAGAGGA GAGAAATGTT GAAAGATCCT 
GAGAGGAGGA ACAAGCTGAT CTCAAATCCT ACAAATTTTA ACCATGTGGC TCATATGGGA 
CCTGGAGATG GCATGCAGAT ACTCAGAGAC CTGCCAATGA ATGTACGTGC TCAGGACAAC 
CGTGCTGGAT TCAGCGGCTC CGTCAGCATC CCCTCCATTA CAAAAAACAG AGCAGAACCA 
GGGCGATCCA TGAGCGCAAG CAGTGGCCTA GGAATGCGGT CGTCCTCTCA GAACGGCAGT 
GCTCTAAGGA GGGAGCTGTC CAGCGGCAGC TACAGCTCTA AACGGCAGAC CATGACTTCA 
CCCTCCGAGA GTTCGCTGTC ATCAGGAGGA GGGATGGACG TGGGGGAAGC GCCGCTCTCT 
CAGTTTGACA GAGAGTGGCA GGCAGTATCG CCCTCGGAGA AGACCCCTGA TCTGAAAAGG 
GCTTTAAGGA CCCTGCTTAG TAAGATGAAG GACTCTGATT CGCCGCGGCA CTCCACTGCC 
TCCAACAGCT CCACCTTCAG CAGCCCGCCG AGCCCCGCCT CCCCACACAA GACAAAGTCC 
CTCTCATTGG AGAGCACAGA CCGCACCGCC TGGGACACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160806.1
CDS	1650..7031
Translation	MSVEVRLKKLEKLIMDGPSQSSGQCFSMETLLDILVCLYDECSSSPIRREKNVLEFLDWAKPFTSKVKQMRLHKEDFEILKVIGRGAFGEVAVVKVKNTDKVFAMKILNKWEMLKRAETACFREERDVLVNGDCQWITTLHYAFQDENNLYLVMDYYVGGDLLTLLSKFEDRLPEDMAKFYLAEMVLAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPLQVTDVSEDAKDLVRRLICSREHRLGQNGIEDFKQHPFFTGIDWDNIRTCEAPYIPEVSSPTDTSNFDVDDDCLKNCETLPPPSHTAFSGHHLPFVGFTYTSKCTLSDRGCLRESVGPAQVDAHVQRSLEESLASEAYERRIRRLEQEKTELTRKLQESTQTVQALQYPDSDAPVNANKEVEIRSLKSEIDILKKQIADSGQMEQQLENASTARRDLEDSSKVVRNLEKQLKSVTQEKDDLHKELLGLAEKVKAQGKELKDAHSQKKMAIKEFAELNEQLSDVRTQKQRLSRQFKDKEEEMEGVSQKLEALRLEIRKAERMRKELEAQAEEHAAEAQKERKVRERTEQYSKQLEEELEGMKQLKQTGPPNGAASSDQQQELMRVRAELEKKSVQYEEEMCRRETLHSSEMKNLQKELRDAEGQHLTHQKEILVLKDKLEKTRRESQVEREEFVCEYKQKYEREKAHLTDENKKLSAEVEKLNGQFEQLSSSHRQLEEEMRELADKKESVAHWEAQITEIIQWVSDEKDARGYLQALATKMTEELEGLRNTSLGARGTDMPWKMRRFAKLDMSARLELQSALDTEIRAKQSIQDELNKVKATNIATECKLQESESRNQELLGEIERLRKETEELRLRRGVKHQDSQNSFLAFLNAPTSALDQFDRSPSFGPGSKTRRLDSADFTPSNTPSRDEDARSRRISRSRSPSTASDMEPIELIDHTSRSLQTPTGRSGYGSLGHSSPKPKAHQFSVKSFNVPTKCNQCTSLMVGLIRQGCTCEACSFSCHVTCADKAPSVCPVAPDQTRGKLGIDTQRGIGTAYEGHLRVPKPTGVKKGWQRVWAVVCDFKLFLYELGEGKGAQPGVVVNQVIDMRDEEFSASSVLESDVIHANRKDIPCIFRVTASQLSASSSQKTCILILADNEQERTKWVCALNELHRILRKNKLKERFVYVPKEAYDSTLPLIKTTQTAAIIDHERVALGNEEGLFVIHVTKDEIIRVGDVKKVHHVELMPTEHILAVISGRNRQVCLVSMAGLDGREVDKNKVADTKNCQLLVSGVVRNVTCFCLAIKRQISCFEINKSKSRHSHLVDIQAPGTVQWMALFNQQLCVGYPSGFMRYSLYGETPPASLLHPDDPTLAFIKTDNLDALCAVEISNKELLLCFSKIAVYVDTHGRRSRQQELMWPAAPTACCYNAPYLSVYSENAVDVFDVNSTEWIQTIPLKKVRPLNMDGSLNLLGLETVRLIYFRNKTAEGDELVVPEVCDNSRKQMVRSMSNKRRFSFRIPEEERLQQRREMLKDPERRNKLISNPTNFNHVAHMGPGDGMQILRDLPMNVRAQDNRAGFSGSVSIPSITKNRAEPGRSMSASSGLGMRSSSQNGSALRRELSSGSYSSKRQTMTSPSESSLSSGGGMDVGEAPLSQFDREWQAVSPSEKTPDLKRALRTLLSKMKDSDSPRHSTASNSSTFSSPPSPASPHKTKSLSLESTDRTAWDT

Target ORF information:

RefSeq Version	XM_005160749.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160749.3

ORF Insert Sequence:

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC 
TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG 
CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC 
CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT 
GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC 
TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA 
GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC 
GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA 
GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA 
CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT 
GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG 
ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC 
CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA 
GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG 
AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG 
AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC 
AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC 
AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT 
CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG 
CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC 
CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG 
GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG 
CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC 
AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC 
AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC 
GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG 
GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC 
ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG 
GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA 
ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG 
CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC 
AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC 
CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG 
AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT 
TCTGCACTGG ATCAGTTTGA TCGCTCACCC TCTTTTGGTC CCGGAAGCAA AACCAGACGT 
CTTGACTCTG CAGATTTCAC CCCTTCCAAC ACTCCATCCA GAGATGAAGA CGCAAGATCT 
CGCCGTATTT CACGCTCTCG CTCACCCTCC ACCGCCAGTG ACATGGAGCC TATAGAGTTG 
ATTGACCACA CATCACGTAG TCTTCAGACC CCAACAGGCA GATCAGGCTA CGGGAGCTTG 
GGACACTCAT CACCTAAGCC CAAGGCACAC CAGTTTAGTG TAAAGTCCTT CAATGTTCCC 
ACTAAATGTA ACCAGTGCAC CTCTCTCATG GTGGGACTCA TCCGTCAGGG CTGCACATGC 
GAAGCCTGCA GCTTCTCCTG TCATGTCACT TGTGCTGATA AAGCCCCTTC TGTGTGCCCC 
GTCGCACCAG ACCAGACCAG GGGCAAACTG GGCATTGACA CTCAGAGGGG CATTGGGACT 
GCATATGAGG GGCATCTTAG GGTGCCGAAG CCCACTGGGG TGAAGAAGGG TTGGCAGAGG 
GTCTGGGCAG TGGTTTGTGA CTTCAAACTT TTTCTGTATG AGCTTGGAGA AGGGAAAGGT 
GCTCAGCCTG GTGTTGTAGT AAACCAGGTC ATTGACATGA GGGATGAAGA GTTTTCCGCC 
AGTTCGGTTC TGGAGTCTGA TGTCATCCAT GCTAATAGGA AGGATATACC CTGTATTTTT 
AGGGTAACAG CATCTCAGCT GTCCGCCTCC TCCAGTCAGA AGACGTGTAT TCTAATTCTG 
GCTGACAACG AGCAAGAAAG GACGAAGTGG GTGTGCGCTC TGAATGAACT GCATCGCATC 
CTGAGGAAAA ACAAGCTGAA GGAGCGTTTC GTCTATGTGC CTAAAGAAGC CTACGACAGC 
ACACTTCCTC TCATCAAAAC CACCCAAACT GCTGCTATTA TAGATCATGA ACGCGTTGCT 
TTGGGCAATG AGGAGGGCCT GTTTGTCATC CACGTCACCA AAGATGAAAT CATTCGTGTG 
GGTGATGTCA AGAAGGTGCA TCATGTAGAA CTGATGCCCA CGGAGCACAT ACTGGCCGTC 
ATCTCGGGCA GGAACCGACA AGTATGTCTG GTCTCCATGG CGGGTCTCGA CGGGAGGGAG 
GTCGACAAGA ATAAGGTGGC AGATACTAAA AACTGCCAGT TGCTGGTGTC CGGTGTGGTT 
CGCAATGTCA CCTGCTTCTG CCTCGCCATT AAACGGCAGA TCTCATGCTT CGAAATTAAC 
AAGAGCAAGT CACGACACTC CCATCTCGTG GACATACAAG CTCCAGGAAC TGTCCAATGG 
ATGGCGCTGT TCAACCAGCA GTTGTGTGTT GGTTACCCAT CAGGCTTCAT GCGCTACAGT 
TTGTATGGAG AAACACCTCC AGCCAGCTTA CTTCATCCAG ATGACCCCAC ACTGGCATTT 
ATTAAAACAG ACAACCTGGA TGCCCTTTGT GCTGTGGAAA TCTCCAATAA GGAACTGCTG 
CTCTGCTTCA GTAAAATAGC AGTGTATGTT GACACACACG GCAGACGCTC GCGCCAGCAG 
GAGCTGATGT GGCCCGCGGC GCCCACCGCC TGCTGCTATA ACGCCCCCTA TCTGTCGGTC 
TACAGTGAAA ATGCAGTGGA TGTGTTTGAT GTGAACTCTA CGGAATGGAT TCAGACCATT 
CCTCTCAAAA AGGTCCGGCC ATTAAACATG GATGGCTCTT TGAATCTCCT GGGCCTAGAA 
ACGGTCAGAC TCATCTACTT CAGAAACAAG ACCGCAGAGG GGGATGAACT GGTGGTGCCT 
GAAGTGTGTG ATAACAGCAG AAAGCAGATG GTGCGAAGCA TGAGCAACAA GAGACGATTC 
TCGTTTCGTA TTCCTGAGGA GGAGAGACTG CAGCAGAGGA GAGAAATGTT GAAAGATCCT 
GAGAGGAGGA ACAAGCTGAT CTCAAATCCT ACAAATTTTA ACCATGTGGC TCATATGGGA 
CCTGGAGATG GCATGCAGAT ACTCAGAGAC CTGCCAATGA ATGTACGTGC TCAGGACAAC 
CGTGCTGGAT TCAGCGGCTC CGTCAGCATC CCCTCCATTA CAAAAAACAG AGCAGAACCA 
GGGCGATCCA TGAGCGCAAG CAGTGGCCTA GGAATGCGGT CGTCCTCTCA GAACGGCAGT 
GCTCTAAGGA GGGAGCTGTC CAGCGGCAGC TACAGCTCTA AACGGCAGAC CATGACTTCA 
CCCTCCGAGA GTTCGCTGTC ATCAGGAGGA GGGATGGACG TGGGGGAAGC GCCGCTCTCT 
CAGTTTGACA GAGAGTGGCA GGCAGTATCG CCCTCGGAGA AGACCCCTGA TCTGAAAAGG 
GCTTTAAGGA CCCTGCTTAG TAAGATGAAG GACTCTGATT CGCCGCGGCA CTCCACTGCC 
TCCAACAGCT CCACCTTCAG CAGCCCGCCG AGCCCCGCCT CCCCACACAA GACAAAGTCC 
CTCTCATTGG AGAGCACAGA CCGCACCGCC TGGGACACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-89p3.3 ( XM_005160750.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160807.1 Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X3, mRNA.

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CloneID	ODa25025
Clone ID Related Accession (Same CDS sequence)	XM_005160750.3
Accession Version	XM_005160750.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase MRCK alpha isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160750.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC 
TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG 
CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC 
CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT 
GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC 
TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA 
GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC 
GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA 
GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA 
CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT 
GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG 
ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC 
CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA 
GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG 
AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG 
AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC 
AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC 
AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT 
CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG 
CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC 
CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG 
GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG 
CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC 
AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC 
AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC 
GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG 
GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC 
ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG 
GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA 
ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG 
CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC 
AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC 
CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG 
AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT 
TCTGCACTGG ATCAGTTTGA TCGCTCACCC TCTTTTGGTC CCGGAAGCAA AACCAGACGT 
CTTGACTCTG CAGATTTCAC CCCTTCCAAC ACTCCATCCA GAGATGAAGA CGCAAGATCT 
CGCCGTATTT CACGCTCTCG CTCACCCTCC ACCGCCAGTG ACATGGAGCC TATAGAGTTG 
ATTGACCACA CATCACGTAG TCTTCAGACC CCAACAGGCA GATCAGGCTA CGGGAGCTTG 
GGACACTCAT CACCTAAGCC CAAGGCACAC CAGTTTAGTG TAAAGTCCTT CAATGTTCCC 
ACTAAATGTA ACCAGTGCAC CTCTCTCATG GTGGGACTCA TCCGTCAGGG CTGCACATGC 
GAAGCCTGCA GCTTCTCCTG TCATGTCACT TGTGCTGATA AAGCCCCTTC TGTGTGCCCC 
GTCGCACCAG ACCAGACCAG GGGCAAACTG GGCATTGACA CTCAGAGGGG CATTGGGACT 
GCATATGAGG GGCATCTTAG GGTGCCGAAG CCCACTGGGG TGAAGAAGGG TTGGCAGAGG 
GTCTGGGCAG TGGTTTGTGA CTTCAAACTT TTTCTGTATG AGCTTGGAGA AGGGAAAGGT 
GCTCAGCCTG GTGTTGTAGT AAACCAGGTC ATTGACATGA GGGATGAAGA GTTTTCCGCC 
AGTTCGGTTC TGGAGTCTGA TGTCATCCAT GCTAATAGGA AGGATATACC CTGTATTTTT 
AGGGTAACAG CATCTCAGCT GTCCGCCTCC TCCAGTCAGA AGACGTGTAT TCTAATTCTG 
GCTGACAACG AGCAAGAAAG GACGAAGTGG GTGTGCGCTC TGAATGAACT GCATCGCATC 
CTGAGGAAAA ACAAGCTGAA GGAGCGTTTC GTCTATGTGC CTAAAGAAGC CTACGACAGC 
ACACTTCCTC TCATCAAAAC CACCCAAACT GCTGCTATTA TAGATCATGA ACGCGTTGCT 
TTGGGCAATG AGGAGGGCCT GTTTGTCATC CACGTCACCA AAGATGAAAT CATTCGTGTG 
GGTGATGTCA AGAAGGTGCA TCATGTAGAA CTGATGCCCA CGGAGCACAT ACTGGCCGTC 
ATCTCGGGCA GGAACCGACA AGTATGTCTG GTCTCCATGG CGGGTCTCGA CGGGAGGGAG 
GTCGACAAGA ATAAGGTGGC AGATACTAAA AACTGCCAGT TGCTGGTGTC CGGTGTGGTT 
CGCAATGTCA CCTGCTTCTG CCTCGCCATT AAACGGCAGA TCTCATGCTT CGAAATTAAC 
AAGAGCAAGT CACGACACTC CCATCTCGTG GACATACAAG CTCCAGGAAC TGTCCAATGG 
ATGGCGCTGT TCAACCAGCA GTTGTGTGTT GGTTACCCAT CAGGCTTCAT GCGCTACAGT 
TTGTATGGAG AAACACCTCC AGCCAGCTTA CTTCATCCAG ATGACCCCAC ACTGGCATTT 
ATTAAAACAG ACAACCTGGA TGCCCTTTGT GCTGTGGAAA TCTCCAATAA GGAACTGCTG 
CTCTGCTTCA GTAAAATAGC AGTGTATGTT GACACACACG GCAGACGCTC GCGCCAGCAG 
GAGCTGATGT GGCCCGCGGC GCCCACCGCC TGCTGCTATA ACGCCCCCTA TCTGTCGGTC 
TACAGTGAAA ATGCAGTGGA TGTGTTTGAT GTGAACTCTA CGGAATGGAT TCAGACCATT 
CCTCTCAAAA AGGTCCGGCC ATTAAACATG GATGGCTCTT TGAATCTCCT GGGCCTAGAA 
ACGGTCAGAC TCATCTACTT CAGAAACAAG ACCGCAGAGG GGGATGAACT GGTGGTGCCT 
GAAGTGTGTG ATAACAGCAG AAAGCAGATG GTGCGAAGCA TGAGCAACAA GAGACGATTC 
TCGTTTCGTA TTCCTGAGGA GGAGAGACTG CAGCAGAGGA GAGAAATGTT GAAAGATCCT 
GAGAGGAGGA ACAAGCTGAT CTCAAATCCT ACAAATTTTA ACCATGTGGC TCATATGGGA 
CCTGGAGATG GCATGCAGAT ACTCAGAGAC CTGCCAATGG ACAACCGTGC TGGATTCAGC 
GGCTCCGTCA GCATCCCCTC CATTACAAAA AACAGAGCAG AACCAGGGCG ATCCATGAGC 
GCAAGCAGTG GCCTAGGAAT GCGGTCGTCC TCTCAGAACG GCAGTGCTCT AAGGAGGGAG 
CTGTCCAGCG GCAGCTACAG CTCTAAACGG CAGACCATGA CTTCACCCTC CGAGAGTTCG 
CTGTCATCAG GAGGAGGGAT GGACGTGGGG GAAGCGCCGC TCTCTCAGTT TGACAGAGAG 
TGGCAGGCAG TATCGCCCTC GGAGAAGACC CCTGATCTGA AAAGGGCTTT AAGGACCCTG 
CTTAGTAAGA TGAAGGACTC TGATTCGCCG CGGCACTCCA CTGCCTCCAA CAGCTCCACC 
TTCAGCAGCC CGCCGAGCCC CGCCTCCCCA CACAAGACAA AGTCCCTCTC ATTGGAGAGC 
ACAGACCGCA CCGCCTGGGA CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160807.1
CDS	1650..7016
Translation	MSVEVRLKKLEKLIMDGPSQSSGQCFSMETLLDILVCLYDECSSSPIRREKNVLEFLDWAKPFTSKVKQMRLHKEDFEILKVIGRGAFGEVAVVKVKNTDKVFAMKILNKWEMLKRAETACFREERDVLVNGDCQWITTLHYAFQDENNLYLVMDYYVGGDLLTLLSKFEDRLPEDMAKFYLAEMVLAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPLQVTDVSEDAKDLVRRLICSREHRLGQNGIEDFKQHPFFTGIDWDNIRTCEAPYIPEVSSPTDTSNFDVDDDCLKNCETLPPPSHTAFSGHHLPFVGFTYTSKCTLSDRGCLRESVGPAQVDAHVQRSLEESLASEAYERRIRRLEQEKTELTRKLQESTQTVQALQYPDSDAPVNANKEVEIRSLKSEIDILKKQIADSGQMEQQLENASTARRDLEDSSKVVRNLEKQLKSVTQEKDDLHKELLGLAEKVKAQGKELKDAHSQKKMAIKEFAELNEQLSDVRTQKQRLSRQFKDKEEEMEGVSQKLEALRLEIRKAERMRKELEAQAEEHAAEAQKERKVRERTEQYSKQLEEELEGMKQLKQTGPPNGAASSDQQQELMRVRAELEKKSVQYEEEMCRRETLHSSEMKNLQKELRDAEGQHLTHQKEILVLKDKLEKTRRESQVEREEFVCEYKQKYEREKAHLTDENKKLSAEVEKLNGQFEQLSSSHRQLEEEMRELADKKESVAHWEAQITEIIQWVSDEKDARGYLQALATKMTEELEGLRNTSLGARGTDMPWKMRRFAKLDMSARLELQSALDTEIRAKQSIQDELNKVKATNIATECKLQESESRNQELLGEIERLRKETEELRLRRGVKHQDSQNSFLAFLNAPTSALDQFDRSPSFGPGSKTRRLDSADFTPSNTPSRDEDARSRRISRSRSPSTASDMEPIELIDHTSRSLQTPTGRSGYGSLGHSSPKPKAHQFSVKSFNVPTKCNQCTSLMVGLIRQGCTCEACSFSCHVTCADKAPSVCPVAPDQTRGKLGIDTQRGIGTAYEGHLRVPKPTGVKKGWQRVWAVVCDFKLFLYELGEGKGAQPGVVVNQVIDMRDEEFSASSVLESDVIHANRKDIPCIFRVTASQLSASSSQKTCILILADNEQERTKWVCALNELHRILRKNKLKERFVYVPKEAYDSTLPLIKTTQTAAIIDHERVALGNEEGLFVIHVTKDEIIRVGDVKKVHHVELMPTEHILAVISGRNRQVCLVSMAGLDGREVDKNKVADTKNCQLLVSGVVRNVTCFCLAIKRQISCFEINKSKSRHSHLVDIQAPGTVQWMALFNQQLCVGYPSGFMRYSLYGETPPASLLHPDDPTLAFIKTDNLDALCAVEISNKELLLCFSKIAVYVDTHGRRSRQQELMWPAAPTACCYNAPYLSVYSENAVDVFDVNSTEWIQTIPLKKVRPLNMDGSLNLLGLETVRLIYFRNKTAEGDELVVPEVCDNSRKQMVRSMSNKRRFSFRIPEEERLQQRREMLKDPERRNKLISNPTNFNHVAHMGPGDGMQILRDLPMDNRAGFSGSVSIPSITKNRAEPGRSMSASSGLGMRSSSQNGSALRRELSSGSYSSKRQTMTSPSESSLSSGGGMDVGEAPLSQFDREWQAVSPSEKTPDLKRALRTLLSKMKDSDSPRHSTASNSSTFSSPPSPASPHKTKSLSLESTDRTAWDT

Target ORF information:

RefSeq Version	XM_005160750.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160750.3

ORF Insert Sequence:

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC 
TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG 
CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC 
CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT 
GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC 
TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA 
GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC 
GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA 
GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA 
CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT 
GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG 
ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC 
CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA 
GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG 
AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG 
AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC 
AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC 
AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT 
CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG 
CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC 
CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG 
GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG 
CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC 
AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC 
AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC 
GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG 
GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC 
ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG 
GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA 
ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG 
CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC 
AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC 
CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG 
AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT 
TCTGCACTGG ATCAGTTTGA TCGCTCACCC TCTTTTGGTC CCGGAAGCAA AACCAGACGT 
CTTGACTCTG CAGATTTCAC CCCTTCCAAC ACTCCATCCA GAGATGAAGA CGCAAGATCT 
CGCCGTATTT CACGCTCTCG CTCACCCTCC ACCGCCAGTG ACATGGAGCC TATAGAGTTG 
ATTGACCACA CATCACGTAG TCTTCAGACC CCAACAGGCA GATCAGGCTA CGGGAGCTTG 
GGACACTCAT CACCTAAGCC CAAGGCACAC CAGTTTAGTG TAAAGTCCTT CAATGTTCCC 
ACTAAATGTA ACCAGTGCAC CTCTCTCATG GTGGGACTCA TCCGTCAGGG CTGCACATGC 
GAAGCCTGCA GCTTCTCCTG TCATGTCACT TGTGCTGATA AAGCCCCTTC TGTGTGCCCC 
GTCGCACCAG ACCAGACCAG GGGCAAACTG GGCATTGACA CTCAGAGGGG CATTGGGACT 
GCATATGAGG GGCATCTTAG GGTGCCGAAG CCCACTGGGG TGAAGAAGGG TTGGCAGAGG 
GTCTGGGCAG TGGTTTGTGA CTTCAAACTT TTTCTGTATG AGCTTGGAGA AGGGAAAGGT 
GCTCAGCCTG GTGTTGTAGT AAACCAGGTC ATTGACATGA GGGATGAAGA GTTTTCCGCC 
AGTTCGGTTC TGGAGTCTGA TGTCATCCAT GCTAATAGGA AGGATATACC CTGTATTTTT 
AGGGTAACAG CATCTCAGCT GTCCGCCTCC TCCAGTCAGA AGACGTGTAT TCTAATTCTG 
GCTGACAACG AGCAAGAAAG GACGAAGTGG GTGTGCGCTC TGAATGAACT GCATCGCATC 
CTGAGGAAAA ACAAGCTGAA GGAGCGTTTC GTCTATGTGC CTAAAGAAGC CTACGACAGC 
ACACTTCCTC TCATCAAAAC CACCCAAACT GCTGCTATTA TAGATCATGA ACGCGTTGCT 
TTGGGCAATG AGGAGGGCCT GTTTGTCATC CACGTCACCA AAGATGAAAT CATTCGTGTG 
GGTGATGTCA AGAAGGTGCA TCATGTAGAA CTGATGCCCA CGGAGCACAT ACTGGCCGTC 
ATCTCGGGCA GGAACCGACA AGTATGTCTG GTCTCCATGG CGGGTCTCGA CGGGAGGGAG 
GTCGACAAGA ATAAGGTGGC AGATACTAAA AACTGCCAGT TGCTGGTGTC CGGTGTGGTT 
CGCAATGTCA CCTGCTTCTG CCTCGCCATT AAACGGCAGA TCTCATGCTT CGAAATTAAC 
AAGAGCAAGT CACGACACTC CCATCTCGTG GACATACAAG CTCCAGGAAC TGTCCAATGG 
ATGGCGCTGT TCAACCAGCA GTTGTGTGTT GGTTACCCAT CAGGCTTCAT GCGCTACAGT 
TTGTATGGAG AAACACCTCC AGCCAGCTTA CTTCATCCAG ATGACCCCAC ACTGGCATTT 
ATTAAAACAG ACAACCTGGA TGCCCTTTGT GCTGTGGAAA TCTCCAATAA GGAACTGCTG 
CTCTGCTTCA GTAAAATAGC AGTGTATGTT GACACACACG GCAGACGCTC GCGCCAGCAG 
GAGCTGATGT GGCCCGCGGC GCCCACCGCC TGCTGCTATA ACGCCCCCTA TCTGTCGGTC 
TACAGTGAAA ATGCAGTGGA TGTGTTTGAT GTGAACTCTA CGGAATGGAT TCAGACCATT 
CCTCTCAAAA AGGTCCGGCC ATTAAACATG GATGGCTCTT TGAATCTCCT GGGCCTAGAA 
ACGGTCAGAC TCATCTACTT CAGAAACAAG ACCGCAGAGG GGGATGAACT GGTGGTGCCT 
GAAGTGTGTG ATAACAGCAG AAAGCAGATG GTGCGAAGCA TGAGCAACAA GAGACGATTC 
TCGTTTCGTA TTCCTGAGGA GGAGAGACTG CAGCAGAGGA GAGAAATGTT GAAAGATCCT 
GAGAGGAGGA ACAAGCTGAT CTCAAATCCT ACAAATTTTA ACCATGTGGC TCATATGGGA 
CCTGGAGATG GCATGCAGAT ACTCAGAGAC CTGCCAATGG ACAACCGTGC TGGATTCAGC 
GGCTCCGTCA GCATCCCCTC CATTACAAAA AACAGAGCAG AACCAGGGCG ATCCATGAGC 
GCAAGCAGTG GCCTAGGAAT GCGGTCGTCC TCTCAGAACG GCAGTGCTCT AAGGAGGGAG 
CTGTCCAGCG GCAGCTACAG CTCTAAACGG CAGACCATGA CTTCACCCTC CGAGAGTTCG 
CTGTCATCAG GAGGAGGGAT GGACGTGGGG GAAGCGCCGC TCTCTCAGTT TGACAGAGAG 
TGGCAGGCAG TATCGCCCTC GGAGAAGACC CCTGATCTGA AAAGGGCTTT AAGGACCCTG 
CTTAGTAAGA TGAAGGACTC TGATTCGCCG CGGCACTCCA CTGCCTCCAA CAGCTCCACC 
TTCAGCAGCC CGCCGAGCCC CGCCTCCCCA CACAAGACAA AGTCCCTCTC ATTGGAGAGC 
ACAGACCGCA CCGCCTGGGA CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-89p3.3 ( XM_005160751.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160808.1 Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X8, mRNA.

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CloneID	ODa25026
Clone ID Related Accession (Same CDS sequence)	XM_005160751.4 , XM_005160751.3
Accession Version	XM_005160751.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase MRCK alpha isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160751.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC 
TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG 
CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC 
CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT 
GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC 
TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA 
GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC 
GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA 
GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA 
CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT 
GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG 
ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC 
CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA 
GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG 
AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG 
AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC 
AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC 
AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT 
CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG 
CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC 
CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG 
GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG 
CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC 
AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC 
AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC 
GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG 
GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC 
ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG 
GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA 
ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG 
CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC 
AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC 
CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG 
AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT 
TCTGCACTGG ATCAGTTTGA TCGCTCACCC TCTTTTGGTC CCGGAAGCAA AACCAGACGT 
CTTGACTCTG CAGATTTCAC CCCTTCCAAC ACTCCATCCA GAGATGAAGA CGCAAGATCT 
CGCCGTATTT CACGCTCTCG CTCACCCTCC ACCGCCAGTG ACATGGAGCC TATAGAGTTG 
ATTGACCACA CATCACGTAG TCTTCAGACC CCAACAGGCA GATCAGGCTA CGGGAGCTTG 
GGACACTCAT CACCTAAGCC CAAGGCACAC CAGTTTAGTG TAAAGTCCTT CAATGTTCCC 
ACTAAATGTA ACCAGTGCAC CTCTCTCATG GTGGGACTCA TCCGTCAGGG CTGCACATGC 
GAAGCCTGCA GCTTCTCCTG TCATGTCACT TGTGCTGATA AAGCCCCTTC TGTGTGCCCC 
GTCGCACCAG ACCAGACCAG GGGCAAACTG GGCATTGACA CTCAGAGGGG CATTGGGACT 
GCATATGAGG GGCATCTTAG GGTGCCGAAG CCCACTGGGG TGAAGAAGGG TTGGCAGAGG 
GTCTGGGCAG TGGTTTGTGA CTTCAAACTT TTTCTGTATG AGCTTGGAGA AGGGAAAGGT 
GCTCAGCCTG GTGTTGTAGT AAACCAGGTC ATTGACATGA GGGATGAAGA GTTTTCCGCC 
AGTTCGGTTC TGGAGTCTGA TGTCATCCAT GCTAATAGGA AGGATATACC CTGTATTTTT 
AGGGTAACAG CATCTCAGCT GTCCGCCTCC TCCAGTCAGA AGACGTGTAT TCTAATTCTG 
GCTGACAACG AGCAAGAAAG GACGAAGTGG GTGTGCGCTC TGAATGAACT GCATCGCATC 
CTGAGGAAAA ACAAGCTGAA GGAGCGTTTC GTCTATGTGC CTAAAGAAGC CTACGACAGC 
ACACTTCCTC TCATCAAAAC CACCCAAACT GCTGCTATTA TAGATCATGA ACGCGTTGCT 
TTGGGCAATG AGGAGGGCCT GTTTGTCATC CACGTCACCA AAGATGAAAT CATTCGTGTG 
GGTGATGTCA AGAAGGTGCA TCATGTAGAA CTGATGCCCA CGGAGCACAT ACTGGCCGTC 
ATCTCGGGCA GGAACCGACA AGTATGTCTG GTCTCCATGG CGGGTCTCGA CGGGAGGGAG 
GTCGACAAGA ATAAGGTGGC AGATACTAAA AACTGCCAGT TGCTGGTGTC CGGTGTGGTT 
CGCAATGTCA CCTGCTTCTG CCTCGCCATT AAACGGCAGA TCTCATGCTT CGAAATTAAC 
AAGAGCAAGT CACGACACTC CCATCTCGTG GACATACAAG CTCCAGGAAC TGTCCAATGG 
ATGGCGCTGT TCAACCAGCA GTTGTGTGTT GGTTACCCAT CAGGCTTCAT GCGCTACAGT 
TTGTATGGAG AAACACCTCC AGCCAGCTTA CTTCATCCAG ATGACCCCAC ACTGGCATTT 
ATTAAAACAG ACAACCTGGA TGCCCTTTGT GCTGTGGAAA TCTCCAATAA GGAACTGCTG 
CTCTGCTTCA GTAAAATAGC AGTGTATGTT GACACACACG GCAGACGCTC GCGCCAGCAG 
GAGCTGATGT GGCCCGCGGC GCCCACCGCC TGCTGCTATA ACGCCCCCTA TCTGTCGGTC 
TACAGTGAAA ATGCAGTGGA TGTGTTTGAT GTGAACTCTA CGGAATGGAT TCAGACCATT 
CCTCTCAAAA AGGTCCGGCC ATTAAACATG GATGGCTCTT TGAATCTCCT GGGCCTAGAA 
ACGGTCAGAC TCATCTACTT CAGAAACAAG ACCGCAGAGG GGGATGAACT GGTGGTGCCT 
GAAGTGTGTG ATAACAGCAG AAAGCAGATG GTGCGAAGCA TGAGCAACAA GAGACGATTC 
TCGTTTCGTA TTCCTGAGGA GGAGAGACTG CAGCAGAGGA GAGAAATGTT GAAAGATCCT 
GAGAGGAGGA ACAAGCTGAT CTCAAATCCT ACAAATTTTA ACCATGTGGC TCATATGGGA 
CCTGGAGATG GCATGCAGAT ACTCAGAGAC CTGCCAATGA ATGTACGTGC TCAGGACAAC 
CGTGCTGGAT TCAGCGGCTC CGTCAGCATC CCCTCCATTA CAAAAAACAG AGCAGAACCA 
GGGCGATCCA TGAGCGCAAG CAGTGGCCTA GGAATGCGGT CGTCCTCTCA GAACGGCAGT 
GCTCTAAGGA GGGAGCTGTC CAGCGGCAGC TACAGCTCTA AACGGCAGAC CATGACTTCA 
CCCTCCGAGA GTTCGCTGTC ATCAGGAGGA GGGATGGACG TGGGGGAAGC GCCGCTCTCT 
CAGTTTGACA GAGAGGACTC TGATTCGCCG CGGCACTCCA CTGCCTCCAA CAGCTCCACC 
TTCAGCAGCC CGCCGAGCCC CGCCTCCCCA CACAAGACAA AGTCCCTCTC ATTGGAGAGC 
ACAGACCGCA CCGCCTGGGA CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160808.1
CDS	1641..6947
Translation	MSVEVRLKKLEKLIMDGPSQSSGQCFSMETLLDILVCLYDECSSSPIRREKNVLEFLDWAKPFTSKVKQMRLHKEDFEILKVIGRGAFGEVAVVKVKNTDKVFAMKILNKWEMLKRAETACFREERDVLVNGDCQWITTLHYAFQDENNLYLVMDYYVGGDLLTLLSKFEDRLPEDMAKFYLAEMVLAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPLQVTDVSEDAKDLVRRLICSREHRLGQNGIEDFKQHPFFTGIDWDNIRTCEAPYIPEVSSPTDTSNFDVDDDCLKNCETLPPPSHTAFSGHHLPFVGFTYTSKCTLSDRGCLRESVGPAQVDAHVQRSLEESLASEAYERRIRRLEQEKTELTRKLQESTQTVQALQYPDSDAPVNANKEVEIRSLKSEIDILKKQIADSGQMEQQLENASTARRDLEDSSKVVRNLEKQLKSVTQEKDDLHKELLGLAEKVKAQGKELKDAHSQKKMAIKEFAELNEQLSDVRTQKQRLSRQFKDKEEEMEGVSQKLEALRLEIRKAERMRKELEAQAEEHAAEAQKERKVRERTEQYSKQLEEELEGMKQLKQTGPPNGAASSDQQQELMRVRAELEKKSVQYEEEMCRRETLHSSEMKNLQKELRDAEGQHLTHQKEILVLKDKLEKTRRESQVEREEFVCEYKQKYEREKAHLTDENKKLSAEVEKLNGQFEQLSSSHRQLEEEMRELADKKESVAHWEAQITEIIQWVSDEKDARGYLQALATKMTEELEGLRNTSLGARGTDMPWKMRRFAKLDMSARLELQSALDTEIRAKQSIQDELNKVKATNIATECKLQESESRNQELLGEIERLRKETEELRLRRGVKHQDSQNSFLAFLNAPTSALDQFDRSPSFGPGSKTRRLDSADFTPSNTPSRDEDARSRRISRSRSPSTASDMEPIELIDHTSRSLQTPTGRSGYGSLGHSSPKPKAHQFSVKSFNVPTKCNQCTSLMVGLIRQGCTCEACSFSCHVTCADKAPSVCPVAPDQTRGKLGIDTQRGIGTAYEGHLRVPKPTGVKKGWQRVWAVVCDFKLFLYELGEGKGAQPGVVVNQVIDMRDEEFSASSVLESDVIHANRKDIPCIFRVTASQLSASSSQKTCILILADNEQERTKWVCALNELHRILRKNKLKERFVYVPKEAYDSTLPLIKTTQTAAIIDHERVALGNEEGLFVIHVTKDEIIRVGDVKKVHHVELMPTEHILAVISGRNRQVCLVSMAGLDGREVDKNKVADTKNCQLLVSGVVRNVTCFCLAIKRQISCFEINKSKSRHSHLVDIQAPGTVQWMALFNQQLCVGYPSGFMRYSLYGETPPASLLHPDDPTLAFIKTDNLDALCAVEISNKELLLCFSKIAVYVDTHGRRSRQQELMWPAAPTACCYNAPYLSVYSENAVDVFDVNSTEWIQTIPLKKVRPLNMDGSLNLLGLETVRLIYFRNKTAEGDELVVPEVCDNSRKQMVRSMSNKRRFSFRIPEEERLQQRREMLKDPERRNKLISNPTNFNHVAHMGPGDGMQILRDLPMNVRAQDNRAGFSGSVSIPSITKNRAEPGRSMSASSGLGMRSSSQNGSALRRELSSGSYSSKRQTMTSPSESSLSSGGGMDVGEAPLSQFDREDSDSPRHSTASNSSTFSSPPSPASPHKTKSLSLESTDRTAWDT

Target ORF information:

RefSeq Version	XM_005160751.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160751.4

ORF Insert Sequence:

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC 
TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG 
CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC 
CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT 
GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC 
TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA 
GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC 
GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA 
GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA 
CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT 
GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG 
ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC 
CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA 
GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG 
AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG 
AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC 
AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC 
AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT 
CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG 
CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC 
CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG 
GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG 
CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC 
AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC 
AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC 
GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG 
GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC 
ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG 
GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA 
ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG 
CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC 
AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC 
CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG 
AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT 
TCTGCACTGG ATCAGTTTGA TCGCTCACCC TCTTTTGGTC CCGGAAGCAA AACCAGACGT 
CTTGACTCTG CAGATTTCAC CCCTTCCAAC ACTCCATCCA GAGATGAAGA CGCAAGATCT 
CGCCGTATTT CACGCTCTCG CTCACCCTCC ACCGCCAGTG ACATGGAGCC TATAGAGTTG 
ATTGACCACA CATCACGTAG TCTTCAGACC CCAACAGGCA GATCAGGCTA CGGGAGCTTG 
GGACACTCAT CACCTAAGCC CAAGGCACAC CAGTTTAGTG TAAAGTCCTT CAATGTTCCC 
ACTAAATGTA ACCAGTGCAC CTCTCTCATG GTGGGACTCA TCCGTCAGGG CTGCACATGC 
GAAGCCTGCA GCTTCTCCTG TCATGTCACT TGTGCTGATA AAGCCCCTTC TGTGTGCCCC 
GTCGCACCAG ACCAGACCAG GGGCAAACTG GGCATTGACA CTCAGAGGGG CATTGGGACT 
GCATATGAGG GGCATCTTAG GGTGCCGAAG CCCACTGGGG TGAAGAAGGG TTGGCAGAGG 
GTCTGGGCAG TGGTTTGTGA CTTCAAACTT TTTCTGTATG AGCTTGGAGA AGGGAAAGGT 
GCTCAGCCTG GTGTTGTAGT AAACCAGGTC ATTGACATGA GGGATGAAGA GTTTTCCGCC 
AGTTCGGTTC TGGAGTCTGA TGTCATCCAT GCTAATAGGA AGGATATACC CTGTATTTTT 
AGGGTAACAG CATCTCAGCT GTCCGCCTCC TCCAGTCAGA AGACGTGTAT TCTAATTCTG 
GCTGACAACG AGCAAGAAAG GACGAAGTGG GTGTGCGCTC TGAATGAACT GCATCGCATC 
CTGAGGAAAA ACAAGCTGAA GGAGCGTTTC GTCTATGTGC CTAAAGAAGC CTACGACAGC 
ACACTTCCTC TCATCAAAAC CACCCAAACT GCTGCTATTA TAGATCATGA ACGCGTTGCT 
TTGGGCAATG AGGAGGGCCT GTTTGTCATC CACGTCACCA AAGATGAAAT CATTCGTGTG 
GGTGATGTCA AGAAGGTGCA TCATGTAGAA CTGATGCCCA CGGAGCACAT ACTGGCCGTC 
ATCTCGGGCA GGAACCGACA AGTATGTCTG GTCTCCATGG CGGGTCTCGA CGGGAGGGAG 
GTCGACAAGA ATAAGGTGGC AGATACTAAA AACTGCCAGT TGCTGGTGTC CGGTGTGGTT 
CGCAATGTCA CCTGCTTCTG CCTCGCCATT AAACGGCAGA TCTCATGCTT CGAAATTAAC 
AAGAGCAAGT CACGACACTC CCATCTCGTG GACATACAAG CTCCAGGAAC TGTCCAATGG 
ATGGCGCTGT TCAACCAGCA GTTGTGTGTT GGTTACCCAT CAGGCTTCAT GCGCTACAGT 
TTGTATGGAG AAACACCTCC AGCCAGCTTA CTTCATCCAG ATGACCCCAC ACTGGCATTT 
ATTAAAACAG ACAACCTGGA TGCCCTTTGT GCTGTGGAAA TCTCCAATAA GGAACTGCTG 
CTCTGCTTCA GTAAAATAGC AGTGTATGTT GACACACACG GCAGACGCTC GCGCCAGCAG 
GAGCTGATGT GGCCCGCGGC GCCCACCGCC TGCTGCTATA ACGCCCCCTA TCTGTCGGTC 
TACAGTGAAA ATGCAGTGGA TGTGTTTGAT GTGAACTCTA CGGAATGGAT TCAGACCATT 
CCTCTCAAAA AGGTCCGGCC ATTAAACATG GATGGCTCTT TGAATCTCCT GGGCCTAGAA 
ACGGTCAGAC TCATCTACTT CAGAAACAAG ACCGCAGAGG GGGATGAACT GGTGGTGCCT 
GAAGTGTGTG ATAACAGCAG AAAGCAGATG GTGCGAAGCA TGAGCAACAA GAGACGATTC 
TCGTTTCGTA TTCCTGAGGA GGAGAGACTG CAGCAGAGGA GAGAAATGTT GAAAGATCCT 
GAGAGGAGGA ACAAGCTGAT CTCAAATCCT ACAAATTTTA ACCATGTGGC TCATATGGGA 
CCTGGAGATG GCATGCAGAT ACTCAGAGAC CTGCCAATGA ATGTACGTGC TCAGGACAAC 
CGTGCTGGAT TCAGCGGCTC CGTCAGCATC CCCTCCATTA CAAAAAACAG AGCAGAACCA 
GGGCGATCCA TGAGCGCAAG CAGTGGCCTA GGAATGCGGT CGTCCTCTCA GAACGGCAGT 
GCTCTAAGGA GGGAGCTGTC CAGCGGCAGC TACAGCTCTA AACGGCAGAC CATGACTTCA 
CCCTCCGAGA GTTCGCTGTC ATCAGGAGGA GGGATGGACG TGGGGGAAGC GCCGCTCTCT 
CAGTTTGACA GAGAGGACTC TGATTCGCCG CGGCACTCCA CTGCCTCCAA CAGCTCCACC 
TTCAGCAGCC CGCCGAGCCC CGCCTCCCCA CACAAGACAA AGTCCCTCTC ATTGGAGAGC 
ACAGACCGCA CCGCCTGGGA CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-89p3.3 ( XM_005160751.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160808.1 Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa25026
Clone ID Related Accession (Same CDS sequence)	XM_005160751.4 , XM_005160751.3
Accession Version	XM_005160751.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase MRCK alpha isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160751.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC 
TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG 
CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC 
CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT 
GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC 
TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA 
GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC 
GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA 
GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA 
CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT 
GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG 
ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC 
CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA 
GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG 
AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG 
AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC 
AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC 
AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT 
CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG 
CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC 
CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG 
GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG 
CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC 
AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC 
AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC 
GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG 
GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC 
ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG 
GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA 
ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG 
CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC 
AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC 
CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG 
AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT 
TCTGCACTGG ATCAGTTTGA TCGCTCACCC TCTTTTGGTC CCGGAAGCAA AACCAGACGT 
CTTGACTCTG CAGATTTCAC CCCTTCCAAC ACTCCATCCA GAGATGAAGA CGCAAGATCT 
CGCCGTATTT CACGCTCTCG CTCACCCTCC ACCGCCAGTG ACATGGAGCC TATAGAGTTG 
ATTGACCACA CATCACGTAG TCTTCAGACC CCAACAGGCA GATCAGGCTA CGGGAGCTTG 
GGACACTCAT CACCTAAGCC CAAGGCACAC CAGTTTAGTG TAAAGTCCTT CAATGTTCCC 
ACTAAATGTA ACCAGTGCAC CTCTCTCATG GTGGGACTCA TCCGTCAGGG CTGCACATGC 
GAAGCCTGCA GCTTCTCCTG TCATGTCACT TGTGCTGATA AAGCCCCTTC TGTGTGCCCC 
GTCGCACCAG ACCAGACCAG GGGCAAACTG GGCATTGACA CTCAGAGGGG CATTGGGACT 
GCATATGAGG GGCATCTTAG GGTGCCGAAG CCCACTGGGG TGAAGAAGGG TTGGCAGAGG 
GTCTGGGCAG TGGTTTGTGA CTTCAAACTT TTTCTGTATG AGCTTGGAGA AGGGAAAGGT 
GCTCAGCCTG GTGTTGTAGT AAACCAGGTC ATTGACATGA GGGATGAAGA GTTTTCCGCC 
AGTTCGGTTC TGGAGTCTGA TGTCATCCAT GCTAATAGGA AGGATATACC CTGTATTTTT 
AGGGTAACAG CATCTCAGCT GTCCGCCTCC TCCAGTCAGA AGACGTGTAT TCTAATTCTG 
GCTGACAACG AGCAAGAAAG GACGAAGTGG GTGTGCGCTC TGAATGAACT GCATCGCATC 
CTGAGGAAAA ACAAGCTGAA GGAGCGTTTC GTCTATGTGC CTAAAGAAGC CTACGACAGC 
ACACTTCCTC TCATCAAAAC CACCCAAACT GCTGCTATTA TAGATCATGA ACGCGTTGCT 
TTGGGCAATG AGGAGGGCCT GTTTGTCATC CACGTCACCA AAGATGAAAT CATTCGTGTG 
GGTGATGTCA AGAAGGTGCA TCATGTAGAA CTGATGCCCA CGGAGCACAT ACTGGCCGTC 
ATCTCGGGCA GGAACCGACA AGTATGTCTG GTCTCCATGG CGGGTCTCGA CGGGAGGGAG 
GTCGACAAGA ATAAGGTGGC AGATACTAAA AACTGCCAGT TGCTGGTGTC CGGTGTGGTT 
CGCAATGTCA CCTGCTTCTG CCTCGCCATT AAACGGCAGA TCTCATGCTT CGAAATTAAC 
AAGAGCAAGT CACGACACTC CCATCTCGTG GACATACAAG CTCCAGGAAC TGTCCAATGG 
ATGGCGCTGT TCAACCAGCA GTTGTGTGTT GGTTACCCAT CAGGCTTCAT GCGCTACAGT 
TTGTATGGAG AAACACCTCC AGCCAGCTTA CTTCATCCAG ATGACCCCAC ACTGGCATTT 
ATTAAAACAG ACAACCTGGA TGCCCTTTGT GCTGTGGAAA TCTCCAATAA GGAACTGCTG 
CTCTGCTTCA GTAAAATAGC AGTGTATGTT GACACACACG GCAGACGCTC GCGCCAGCAG 
GAGCTGATGT GGCCCGCGGC GCCCACCGCC TGCTGCTATA ACGCCCCCTA TCTGTCGGTC 
TACAGTGAAA ATGCAGTGGA TGTGTTTGAT GTGAACTCTA CGGAATGGAT TCAGACCATT 
CCTCTCAAAA AGGTCCGGCC ATTAAACATG GATGGCTCTT TGAATCTCCT GGGCCTAGAA 
ACGGTCAGAC TCATCTACTT CAGAAACAAG ACCGCAGAGG GGGATGAACT GGTGGTGCCT 
GAAGTGTGTG ATAACAGCAG AAAGCAGATG GTGCGAAGCA TGAGCAACAA GAGACGATTC 
TCGTTTCGTA TTCCTGAGGA GGAGAGACTG CAGCAGAGGA GAGAAATGTT GAAAGATCCT 
GAGAGGAGGA ACAAGCTGAT CTCAAATCCT ACAAATTTTA ACCATGTGGC TCATATGGGA 
CCTGGAGATG GCATGCAGAT ACTCAGAGAC CTGCCAATGA ATGTACGTGC TCAGGACAAC 
CGTGCTGGAT TCAGCGGCTC CGTCAGCATC CCCTCCATTA CAAAAAACAG AGCAGAACCA 
GGGCGATCCA TGAGCGCAAG CAGTGGCCTA GGAATGCGGT CGTCCTCTCA GAACGGCAGT 
GCTCTAAGGA GGGAGCTGTC CAGCGGCAGC TACAGCTCTA AACGGCAGAC CATGACTTCA 
CCCTCCGAGA GTTCGCTGTC ATCAGGAGGA GGGATGGACG TGGGGGAAGC GCCGCTCTCT 
CAGTTTGACA GAGAGGACTC TGATTCGCCG CGGCACTCCA CTGCCTCCAA CAGCTCCACC 
TTCAGCAGCC CGCCGAGCCC CGCCTCCCCA CACAAGACAA AGTCCCTCTC ATTGGAGAGC 
ACAGACCGCA CCGCCTGGGA CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160808.1
CDS	1650..6956
Translation	MSVEVRLKKLEKLIMDGPSQSSGQCFSMETLLDILVCLYDECSSSPIRREKNVLEFLDWAKPFTSKVKQMRLHKEDFEILKVIGRGAFGEVAVVKVKNTDKVFAMKILNKWEMLKRAETACFREERDVLVNGDCQWITTLHYAFQDENNLYLVMDYYVGGDLLTLLSKFEDRLPEDMAKFYLAEMVLAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPLQVTDVSEDAKDLVRRLICSREHRLGQNGIEDFKQHPFFTGIDWDNIRTCEAPYIPEVSSPTDTSNFDVDDDCLKNCETLPPPSHTAFSGHHLPFVGFTYTSKCTLSDRGCLRESVGPAQVDAHVQRSLEESLASEAYERRIRRLEQEKTELTRKLQESTQTVQALQYPDSDAPVNANKEVEIRSLKSEIDILKKQIADSGQMEQQLENASTARRDLEDSSKVVRNLEKQLKSVTQEKDDLHKELLGLAEKVKAQGKELKDAHSQKKMAIKEFAELNEQLSDVRTQKQRLSRQFKDKEEEMEGVSQKLEALRLEIRKAERMRKELEAQAEEHAAEAQKERKVRERTEQYSKQLEEELEGMKQLKQTGPPNGAASSDQQQELMRVRAELEKKSVQYEEEMCRRETLHSSEMKNLQKELRDAEGQHLTHQKEILVLKDKLEKTRRESQVEREEFVCEYKQKYEREKAHLTDENKKLSAEVEKLNGQFEQLSSSHRQLEEEMRELADKKESVAHWEAQITEIIQWVSDEKDARGYLQALATKMTEELEGLRNTSLGARGTDMPWKMRRFAKLDMSARLELQSALDTEIRAKQSIQDELNKVKATNIATECKLQESESRNQELLGEIERLRKETEELRLRRGVKHQDSQNSFLAFLNAPTSALDQFDRSPSFGPGSKTRRLDSADFTPSNTPSRDEDARSRRISRSRSPSTASDMEPIELIDHTSRSLQTPTGRSGYGSLGHSSPKPKAHQFSVKSFNVPTKCNQCTSLMVGLIRQGCTCEACSFSCHVTCADKAPSVCPVAPDQTRGKLGIDTQRGIGTAYEGHLRVPKPTGVKKGWQRVWAVVCDFKLFLYELGEGKGAQPGVVVNQVIDMRDEEFSASSVLESDVIHANRKDIPCIFRVTASQLSASSSQKTCILILADNEQERTKWVCALNELHRILRKNKLKERFVYVPKEAYDSTLPLIKTTQTAAIIDHERVALGNEEGLFVIHVTKDEIIRVGDVKKVHHVELMPTEHILAVISGRNRQVCLVSMAGLDGREVDKNKVADTKNCQLLVSGVVRNVTCFCLAIKRQISCFEINKSKSRHSHLVDIQAPGTVQWMALFNQQLCVGYPSGFMRYSLYGETPPASLLHPDDPTLAFIKTDNLDALCAVEISNKELLLCFSKIAVYVDTHGRRSRQQELMWPAAPTACCYNAPYLSVYSENAVDVFDVNSTEWIQTIPLKKVRPLNMDGSLNLLGLETVRLIYFRNKTAEGDELVVPEVCDNSRKQMVRSMSNKRRFSFRIPEEERLQQRREMLKDPERRNKLISNPTNFNHVAHMGPGDGMQILRDLPMNVRAQDNRAGFSGSVSIPSITKNRAEPGRSMSASSGLGMRSSSQNGSALRRELSSGSYSSKRQTMTSPSESSLSSGGGMDVGEAPLSQFDREDSDSPRHSTASNSSTFSSPPSPASPHKTKSLSLESTDRTAWDT

Target ORF information:

RefSeq Version	XM_005160751.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160751.3

ORF Insert Sequence:

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC 
TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG 
CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC 
CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT 
GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC 
TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA 
GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC 
GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA 
GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA 
CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT 
GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG 
ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC 
CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA 
GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG 
AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG 
AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC 
AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC 
AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT 
CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG 
CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC 
CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG 
GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG 
CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC 
AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC 
AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC 
GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG 
GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC 
ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG 
GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA 
ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG 
CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC 
AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC 
CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG 
AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT 
TCTGCACTGG ATCAGTTTGA TCGCTCACCC TCTTTTGGTC CCGGAAGCAA AACCAGACGT 
CTTGACTCTG CAGATTTCAC CCCTTCCAAC ACTCCATCCA GAGATGAAGA CGCAAGATCT 
CGCCGTATTT CACGCTCTCG CTCACCCTCC ACCGCCAGTG ACATGGAGCC TATAGAGTTG 
ATTGACCACA CATCACGTAG TCTTCAGACC CCAACAGGCA GATCAGGCTA CGGGAGCTTG 
GGACACTCAT CACCTAAGCC CAAGGCACAC CAGTTTAGTG TAAAGTCCTT CAATGTTCCC 
ACTAAATGTA ACCAGTGCAC CTCTCTCATG GTGGGACTCA TCCGTCAGGG CTGCACATGC 
GAAGCCTGCA GCTTCTCCTG TCATGTCACT TGTGCTGATA AAGCCCCTTC TGTGTGCCCC 
GTCGCACCAG ACCAGACCAG GGGCAAACTG GGCATTGACA CTCAGAGGGG CATTGGGACT 
GCATATGAGG GGCATCTTAG GGTGCCGAAG CCCACTGGGG TGAAGAAGGG TTGGCAGAGG 
GTCTGGGCAG TGGTTTGTGA CTTCAAACTT TTTCTGTATG AGCTTGGAGA AGGGAAAGGT 
GCTCAGCCTG GTGTTGTAGT AAACCAGGTC ATTGACATGA GGGATGAAGA GTTTTCCGCC 
AGTTCGGTTC TGGAGTCTGA TGTCATCCAT GCTAATAGGA AGGATATACC CTGTATTTTT 
AGGGTAACAG CATCTCAGCT GTCCGCCTCC TCCAGTCAGA AGACGTGTAT TCTAATTCTG 
GCTGACAACG AGCAAGAAAG GACGAAGTGG GTGTGCGCTC TGAATGAACT GCATCGCATC 
CTGAGGAAAA ACAAGCTGAA GGAGCGTTTC GTCTATGTGC CTAAAGAAGC CTACGACAGC 
ACACTTCCTC TCATCAAAAC CACCCAAACT GCTGCTATTA TAGATCATGA ACGCGTTGCT 
TTGGGCAATG AGGAGGGCCT GTTTGTCATC CACGTCACCA AAGATGAAAT CATTCGTGTG 
GGTGATGTCA AGAAGGTGCA TCATGTAGAA CTGATGCCCA CGGAGCACAT ACTGGCCGTC 
ATCTCGGGCA GGAACCGACA AGTATGTCTG GTCTCCATGG CGGGTCTCGA CGGGAGGGAG 
GTCGACAAGA ATAAGGTGGC AGATACTAAA AACTGCCAGT TGCTGGTGTC CGGTGTGGTT 
CGCAATGTCA CCTGCTTCTG CCTCGCCATT AAACGGCAGA TCTCATGCTT CGAAATTAAC 
AAGAGCAAGT CACGACACTC CCATCTCGTG GACATACAAG CTCCAGGAAC TGTCCAATGG 
ATGGCGCTGT TCAACCAGCA GTTGTGTGTT GGTTACCCAT CAGGCTTCAT GCGCTACAGT 
TTGTATGGAG AAACACCTCC AGCCAGCTTA CTTCATCCAG ATGACCCCAC ACTGGCATTT 
ATTAAAACAG ACAACCTGGA TGCCCTTTGT GCTGTGGAAA TCTCCAATAA GGAACTGCTG 
CTCTGCTTCA GTAAAATAGC AGTGTATGTT GACACACACG GCAGACGCTC GCGCCAGCAG 
GAGCTGATGT GGCCCGCGGC GCCCACCGCC TGCTGCTATA ACGCCCCCTA TCTGTCGGTC 
TACAGTGAAA ATGCAGTGGA TGTGTTTGAT GTGAACTCTA CGGAATGGAT TCAGACCATT 
CCTCTCAAAA AGGTCCGGCC ATTAAACATG GATGGCTCTT TGAATCTCCT GGGCCTAGAA 
ACGGTCAGAC TCATCTACTT CAGAAACAAG ACCGCAGAGG GGGATGAACT GGTGGTGCCT 
GAAGTGTGTG ATAACAGCAG AAAGCAGATG GTGCGAAGCA TGAGCAACAA GAGACGATTC 
TCGTTTCGTA TTCCTGAGGA GGAGAGACTG CAGCAGAGGA GAGAAATGTT GAAAGATCCT 
GAGAGGAGGA ACAAGCTGAT CTCAAATCCT ACAAATTTTA ACCATGTGGC TCATATGGGA 
CCTGGAGATG GCATGCAGAT ACTCAGAGAC CTGCCAATGA ATGTACGTGC TCAGGACAAC 
CGTGCTGGAT TCAGCGGCTC CGTCAGCATC CCCTCCATTA CAAAAAACAG AGCAGAACCA 
GGGCGATCCA TGAGCGCAAG CAGTGGCCTA GGAATGCGGT CGTCCTCTCA GAACGGCAGT 
GCTCTAAGGA GGGAGCTGTC CAGCGGCAGC TACAGCTCTA AACGGCAGAC CATGACTTCA 
CCCTCCGAGA GTTCGCTGTC ATCAGGAGGA GGGATGGACG TGGGGGAAGC GCCGCTCTCT 
CAGTTTGACA GAGAGGACTC TGATTCGCCG CGGCACTCCA CTGCCTCCAA CAGCTCCACC 
TTCAGCAGCC CGCCGAGCCC CGCCTCCCCA CACAAGACAA AGTCCCTCTC ATTGGAGAGC 
ACAGACCGCA CCGCCTGGGA CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-89p3.3 ( XM_009294768.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293043.1 Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X4, mRNA.

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CloneID	ODa44774
Clone ID Related Accession (Same CDS sequence)	XM_009294768.2
Accession Version	XM_009294768.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase MRCK alpha isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294768.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC 
TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG 
CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC 
CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT 
GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC 
TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA 
GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC 
GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA 
GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA 
CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT 
GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG 
ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC 
CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA 
GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG 
AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG 
AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC 
AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC 
AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT 
CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG 
CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC 
CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG 
GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG 
CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC 
AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC 
AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC 
GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG 
GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC 
ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG 
GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA 
ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG 
CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC 
AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC 
CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG 
AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT 
TCTGCACTGG ATCAGTTTGA TCTTGACTCT GCAGATTTCA CCCCTTCCAA CACTCCATCC 
AGAGATGAAG ACGCAAGATC TCGCCGTATT TCACGCTCTC GCTCACCCTC CACCGCCAGT 
GACATGGAGC CTATAGAGTT GATTGACCAC ACATCACGTA GTCTTCAGAC CCCAACAGGC 
AGATCAGGCT ACGGGAGCTT GGGACACTCA TCACCTAAGC CCAAGGCACA CCAGTTTAGT 
GTAAAGTCCT TCAATGTTCC CACTAAATGT AACCAGTGCA CCTCTCTCAT GGTGGGACTC 
ATCCGTCAGG GCTGCACATG CGAAGCCTGC AGCTTCTCCT GTCATGTCAC TTGTGCTGAT 
AAAGCCCCTT CTGTGTGCCC CGTCGCACCA GACCAGACCA GGGGCAAACT GGGCATTGAC 
ACTCAGAGGG GCATTGGGAC TGCATATGAG GGGCATCTTA GGGTGCCGAA GCCCACTGGG 
GTGAAGAAGG GTTGGCAGAG GGTCTGGGCA GTGGTTTGTG ACTTCAAACT TTTTCTGTAT 
GAGCTTGGAG AAGGGAAAGG TGCTCAGCCT GGTGTTGTAG TAAACCAGGT CATTGACATG 
AGGGATGAAG AGTTTTCCGC CAGTTCGGTT CTGGAGTCTG ATGTCATCCA TGCTAATAGG 
AAGGATATAC CCTGTATTTT TAGGGTAACA GCATCTCAGC TGTCCGCCTC CTCCAGTCAG 
AAGACGTGTA TTCTAATTCT GGCTGACAAC GAGCAAGAAA GGACGAAGTG GGTGTGCGCT 
CTGAATGAAC TGCATCGCAT CCTGAGGAAA AACAAGCTGA AGGAGCGTTT CGTCTATGTG 
CCTAAAGAAG CCTACGACAG CACACTTCCT CTCATCAAAA CCACCCAAAC TGCTGCTATT 
ATAGATCATG AACGCGTTGC TTTGGGCAAT GAGGAGGGCC TGTTTGTCAT CCACGTCACC 
AAAGATGAAA TCATTCGTGT GGGTGATGTC AAGAAGGTGC ATCATGTAGA ACTGATGCCC 
ACGGAGCACA TACTGGCCGT CATCTCGGGC AGGAACCGAC AAGTATGTCT GGTCTCCATG 
GCGGGTCTCG ACGGGAGGGA GGTCGACAAG AATAAGGTGG CAGATACTAA AAACTGCCAG 
TTGCTGGTGT CCGGTGTGGT TCGCAATGTC ACCTGCTTCT GCCTCGCCAT TAAACGGCAG 
ATCTCATGCT TCGAAATTAA CAAGAGCAAG TCACGACACT CCCATCTCGT GGACATACAA 
GCTCCAGGAA CTGTCCAATG GATGGCGCTG TTCAACCAGC AGTTGTGTGT TGGTTACCCA 
TCAGGCTTCA TGCGCTACAG TTTGTATGGA GAAACACCTC CAGCCAGCTT ACTTCATCCA 
GATGACCCCA CACTGGCATT TATTAAAACA GACAACCTGG ATGCCCTTTG TGCTGTGGAA 
ATCTCCAATA AGGAACTGCT GCTCTGCTTC AGTAAAATAG CAGTGTATGT TGACACACAC 
GGCAGACGCT CGCGCCAGCA GGAGCTGATG TGGCCCGCGG CGCCCACCGC CTGCTGCTAT 
AACGCCCCCT ATCTGTCGGT CTACAGTGAA AATGCAGTGG ATGTGTTTGA TGTGAACTCT 
ACGGAATGGA TTCAGACCAT TCCTCTCAAA AAGGTCCGGC CATTAAACAT GGATGGCTCT 
TTGAATCTCC TGGGCCTAGA AACGGTCAGA CTCATCTACT TCAGAAACAA GACCGCAGAG 
GGGGATGAAC TGGTGGTGCC TGAAGTGTGT GATAACAGCA GAAAGCAGAT GGTGCGAAGC 
ATGAGCAACA AGAGACGATT CTCGTTTCGT ATTCCTGAGG AGGAGAGACT GCAGCAGAGG 
AGAGAAATGT TGAAAGATCC TGAGAGGAGG AACAAGCTGA TCTCAAATCC TACAAATTTT 
AACCATGTGG CTCATATGGG ACCTGGAGAT GGCATGCAGA TACTCAGAGA CCTGCCAATG 
AATGTACGTG CTCAGGACAA CCGTGCTGGA TTCAGCGGCT CCGTCAGCAT CCCCTCCATT 
ACAAAAAACA GAGCAGAACC AGGGCGATCC ATGAGCGCAA GCAGTGGCCT AGGAATGCGG 
TCGTCCTCTC AGAACGGCAG TGCTCTAAGG AGGGAGCTGT CCAGCGGCAG CTACAGCTCT 
AAACGGCAGA CCATGACTTC ACCCTCCGAG AGTTCGCTGT CATCAGGAGG AGGGATGGAC 
GTGGGGGAAG CGCCGCTCTC TCAGTTTGAC AGAGAGTGGC AGGCAGTATC GCCCTCGGAG 
AAGACCCCTG ATCTGAAAAG GGCTTTAAGG ACCCTGCTTA GTAAGATGAA GGACTCTGAT 
TCGCCGCGGC ACTCCACTGC CTCCAACAGC TCCACCTTCA GCAGCCCGCC GAGCCCCGCC 
TCCCCACACA AGACAAAGTC CCTCTCATTG GAGAGCACAG ACCGCACCGC CTGGGACACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293043.1
CDS	1650..6992
Translation	MSVEVRLKKLEKLIMDGPSQSSGQCFSMETLLDILVCLYDECSSSPIRREKNVLEFLDWAKPFTSKVKQMRLHKEDFEILKVIGRGAFGEVAVVKVKNTDKVFAMKILNKWEMLKRAETACFREERDVLVNGDCQWITTLHYAFQDENNLYLVMDYYVGGDLLTLLSKFEDRLPEDMAKFYLAEMVLAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPLQVTDVSEDAKDLVRRLICSREHRLGQNGIEDFKQHPFFTGIDWDNIRTCEAPYIPEVSSPTDTSNFDVDDDCLKNCETLPPPSHTAFSGHHLPFVGFTYTSKCTLSDRGCLRESVGPAQVDAHVQRSLEESLASEAYERRIRRLEQEKTELTRKLQESTQTVQALQYPDSDAPVNANKEVEIRSLKSEIDILKKQIADSGQMEQQLENASTARRDLEDSSKVVRNLEKQLKSVTQEKDDLHKELLGLAEKVKAQGKELKDAHSQKKMAIKEFAELNEQLSDVRTQKQRLSRQFKDKEEEMEGVSQKLEALRLEIRKAERMRKELEAQAEEHAAEAQKERKVRERTEQYSKQLEEELEGMKQLKQTGPPNGAASSDQQQELMRVRAELEKKSVQYEEEMCRRETLHSSEMKNLQKELRDAEGQHLTHQKEILVLKDKLEKTRRESQVEREEFVCEYKQKYEREKAHLTDENKKLSAEVEKLNGQFEQLSSSHRQLEEEMRELADKKESVAHWEAQITEIIQWVSDEKDARGYLQALATKMTEELEGLRNTSLGARGTDMPWKMRRFAKLDMSARLELQSALDTEIRAKQSIQDELNKVKATNIATECKLQESESRNQELLGEIERLRKETEELRLRRGVKHQDSQNSFLAFLNAPTSALDQFDLDSADFTPSNTPSRDEDARSRRISRSRSPSTASDMEPIELIDHTSRSLQTPTGRSGYGSLGHSSPKPKAHQFSVKSFNVPTKCNQCTSLMVGLIRQGCTCEACSFSCHVTCADKAPSVCPVAPDQTRGKLGIDTQRGIGTAYEGHLRVPKPTGVKKGWQRVWAVVCDFKLFLYELGEGKGAQPGVVVNQVIDMRDEEFSASSVLESDVIHANRKDIPCIFRVTASQLSASSSQKTCILILADNEQERTKWVCALNELHRILRKNKLKERFVYVPKEAYDSTLPLIKTTQTAAIIDHERVALGNEEGLFVIHVTKDEIIRVGDVKKVHHVELMPTEHILAVISGRNRQVCLVSMAGLDGREVDKNKVADTKNCQLLVSGVVRNVTCFCLAIKRQISCFEINKSKSRHSHLVDIQAPGTVQWMALFNQQLCVGYPSGFMRYSLYGETPPASLLHPDDPTLAFIKTDNLDALCAVEISNKELLLCFSKIAVYVDTHGRRSRQQELMWPAAPTACCYNAPYLSVYSENAVDVFDVNSTEWIQTIPLKKVRPLNMDGSLNLLGLETVRLIYFRNKTAEGDELVVPEVCDNSRKQMVRSMSNKRRFSFRIPEEERLQQRREMLKDPERRNKLISNPTNFNHVAHMGPGDGMQILRDLPMNVRAQDNRAGFSGSVSIPSITKNRAEPGRSMSASSGLGMRSSSQNGSALRRELSSGSYSSKRQTMTSPSESSLSSGGGMDVGEAPLSQFDREWQAVSPSEKTPDLKRALRTLLSKMKDSDSPRHSTASNSSTFSSPPSPASPHKTKSLSLESTDRTAWDT

Target ORF information:

RefSeq Version	XM_009294768.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294768.2

ORF Insert Sequence:

ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC 
GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC 
AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG 
AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC 
AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA 
TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA 
CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA 
GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC 
TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA 
GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT 
GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC 
CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG 
CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG 
CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC 
TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG 
CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC 
CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT 
GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC 
TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA 
GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC 
GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA 
GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA 
CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT 
GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG 
ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC 
CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA 
GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG 
AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG 
AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC 
AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC 
AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT 
CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG 
CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC 
CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG 
GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG 
CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC 
AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC 
AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC 
GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG 
GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC 
ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG 
GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA 
ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG 
CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC 
AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC 
CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG 
AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT 
TCTGCACTGG ATCAGTTTGA TCTTGACTCT GCAGATTTCA CCCCTTCCAA CACTCCATCC 
AGAGATGAAG ACGCAAGATC TCGCCGTATT TCACGCTCTC GCTCACCCTC CACCGCCAGT 
GACATGGAGC CTATAGAGTT GATTGACCAC ACATCACGTA GTCTTCAGAC CCCAACAGGC 
AGATCAGGCT ACGGGAGCTT GGGACACTCA TCACCTAAGC CCAAGGCACA CCAGTTTAGT 
GTAAAGTCCT TCAATGTTCC CACTAAATGT AACCAGTGCA CCTCTCTCAT GGTGGGACTC 
ATCCGTCAGG GCTGCACATG CGAAGCCTGC AGCTTCTCCT GTCATGTCAC TTGTGCTGAT 
AAAGCCCCTT CTGTGTGCCC CGTCGCACCA GACCAGACCA GGGGCAAACT GGGCATTGAC 
ACTCAGAGGG GCATTGGGAC TGCATATGAG GGGCATCTTA GGGTGCCGAA GCCCACTGGG 
GTGAAGAAGG GTTGGCAGAG GGTCTGGGCA GTGGTTTGTG ACTTCAAACT TTTTCTGTAT 
GAGCTTGGAG AAGGGAAAGG TGCTCAGCCT GGTGTTGTAG TAAACCAGGT CATTGACATG 
AGGGATGAAG AGTTTTCCGC CAGTTCGGTT CTGGAGTCTG ATGTCATCCA TGCTAATAGG 
AAGGATATAC CCTGTATTTT TAGGGTAACA GCATCTCAGC TGTCCGCCTC CTCCAGTCAG 
AAGACGTGTA TTCTAATTCT GGCTGACAAC GAGCAAGAAA GGACGAAGTG GGTGTGCGCT 
CTGAATGAAC TGCATCGCAT CCTGAGGAAA AACAAGCTGA AGGAGCGTTT CGTCTATGTG 
CCTAAAGAAG CCTACGACAG CACACTTCCT CTCATCAAAA CCACCCAAAC TGCTGCTATT 
ATAGATCATG AACGCGTTGC TTTGGGCAAT GAGGAGGGCC TGTTTGTCAT CCACGTCACC 
AAAGATGAAA TCATTCGTGT GGGTGATGTC AAGAAGGTGC ATCATGTAGA ACTGATGCCC 
ACGGAGCACA TACTGGCCGT CATCTCGGGC AGGAACCGAC AAGTATGTCT GGTCTCCATG 
GCGGGTCTCG ACGGGAGGGA GGTCGACAAG AATAAGGTGG CAGATACTAA AAACTGCCAG 
TTGCTGGTGT CCGGTGTGGT TCGCAATGTC ACCTGCTTCT GCCTCGCCAT TAAACGGCAG 
ATCTCATGCT TCGAAATTAA CAAGAGCAAG TCACGACACT CCCATCTCGT GGACATACAA 
GCTCCAGGAA CTGTCCAATG GATGGCGCTG TTCAACCAGC AGTTGTGTGT TGGTTACCCA 
TCAGGCTTCA TGCGCTACAG TTTGTATGGA GAAACACCTC CAGCCAGCTT ACTTCATCCA 
GATGACCCCA CACTGGCATT TATTAAAACA GACAACCTGG ATGCCCTTTG TGCTGTGGAA 
ATCTCCAATA AGGAACTGCT GCTCTGCTTC AGTAAAATAG CAGTGTATGT TGACACACAC 
GGCAGACGCT CGCGCCAGCA GGAGCTGATG TGGCCCGCGG CGCCCACCGC CTGCTGCTAT 
AACGCCCCCT ATCTGTCGGT CTACAGTGAA AATGCAGTGG ATGTGTTTGA TGTGAACTCT 
ACGGAATGGA TTCAGACCAT TCCTCTCAAA AAGGTCCGGC CATTAAACAT GGATGGCTCT 
TTGAATCTCC TGGGCCTAGA AACGGTCAGA CTCATCTACT TCAGAAACAA GACCGCAGAG 
GGGGATGAAC TGGTGGTGCC TGAAGTGTGT GATAACAGCA GAAAGCAGAT GGTGCGAAGC 
ATGAGCAACA AGAGACGATT CTCGTTTCGT ATTCCTGAGG AGGAGAGACT GCAGCAGAGG 
AGAGAAATGT TGAAAGATCC TGAGAGGAGG AACAAGCTGA TCTCAAATCC TACAAATTTT 
AACCATGTGG CTCATATGGG ACCTGGAGAT GGCATGCAGA TACTCAGAGA CCTGCCAATG 
AATGTACGTG CTCAGGACAA CCGTGCTGGA TTCAGCGGCT CCGTCAGCAT CCCCTCCATT 
ACAAAAAACA GAGCAGAACC AGGGCGATCC ATGAGCGCAA GCAGTGGCCT AGGAATGCGG 
TCGTCCTCTC AGAACGGCAG TGCTCTAAGG AGGGAGCTGT CCAGCGGCAG CTACAGCTCT 
AAACGGCAGA CCATGACTTC ACCCTCCGAG AGTTCGCTGT CATCAGGAGG AGGGATGGAC 
GTGGGGGAAG CGCCGCTCTC TCAGTTTGAC AGAGAGTGGC AGGCAGTATC GCCCTCGGAG 
AAGACCCCTG ATCTGAAAAG GGCTTTAAGG ACCCTGCTTA GTAAGATGAA GGACTCTGAT 
TCGCCGCGGC ACTCCACTGC CTCCAACAGC TCCACCTTCA GCAGCCCGCC GAGCCCCGCC 
TCCCCACACA AGACAAAGTC CCTCTCATTG GAGAGCACAG ACCGCACCGC CTGGGACACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	si:ch211-89p3.3
Entrez Gene ID	566003
Full Name	si:ch211-89p3.3
Synonyms	fb82d10,wu:fb82d10,zgc:174985
General protein information	Preferred Names si:ch211-89p3.3 Names serine/threonine-protein kinase MRCK alpha
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Homo sapiens (human)	CDC42BPA	NP_003598.2
	CDC42BPA	XP_002801950.1
Canis lupus familiaris (dog)	CDC42BPA	XP_005622674.1
Gallus gallus (chicken)	CDC42BPA	NP_001186372.1
Xenopus tropicalis (tropical clawed frog)	cdc42bpa	XP_002938889.2
Pan troglodytes (chimpanzee)	CDC42BPA	XP_003949757.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012090	XP_320436.4
Rattus norvegicus (Norway rat)	Cdc42bpa	NP_446109.1
Danio rerio (zebrafish)	si:ch211-89p3.3	NP_001038559.1
Drosophila melanogaster (fruit fly)	gek	NP_523837.2
Caenorhabditis elegans (roundworm)	mrck-1	NP_504599.2
Mus musculus (house mouse)	Cdc42bpa	NP_001028457.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

si:ch211-89p3.3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

ORF Insert Sequence:

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