si:ch211-89p3.3 cDNA ORF clone, Danio rerio(zebrafish)

The following si:ch211-89p3.3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the si:ch211-89p3.3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa44775 XM_009294769.2
Latest version!
Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa150649 XM_021468481.1
Latest version!
Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa150650 XM_021468482.1
Latest version!
Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa150651 XM_021468483.1
Latest version!
Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa150652 XM_021468485.1
Latest version!
Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa150653 XM_021468486.1
Latest version!
Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa56577 XM_017352506.2
Latest version!
Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa56578 XM_017352507.2
Latest version!
Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa33549 NM_001045094.1
Latest version!
Danio rerio CDC42 binding protein kinase alpha (DMPK-like) b (cdc42bpab), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa25024 XM_005160749.4
Latest version!
Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa25025 XM_005160750.3
Latest version!
Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa25026 XM_005160751.4
Latest version!
Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa44774 XM_009294768.2
Latest version!
Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
View Old Accession Versions >>
ODa56577 XM_017352506.1 Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa25026 XM_005160751.3 Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa25024 XM_005160749.3 Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa56578 XM_017352507.1 Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa44775
    Clone ID Related Accession (Same CDS sequence) XM_009294769.2
    Accession Version XM_009294769.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2988bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product serine/threonine-protein kinase MRCK alpha isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294769.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC
    TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG
    CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC
    CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT
    GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC
    TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA
    GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC
    GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA
    GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA
    CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT
    GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG
    ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC
    CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA
    GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG
    AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG
    AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC
    AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC
    AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT
    CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG
    CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC
    CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG
    GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG
    CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC
    AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC
    AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC
    GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG
    GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC
    ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG
    GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA
    ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG
    CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC
    AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC
    CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG
    AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT
    TCTGCACTGG ATCAGTTTGA TGACTCATTT TCTTCCTCTT CATCTTCTTT AGTTGAATTT
    TGGGAAGATG TAAGTTCTCC AGCCATGTTA TTGCAGAAGT GTCTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293044.1
    CDS1650..4637
    Translation

    Target ORF information:

    RefSeq Version XM_009294769.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294769.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC
    TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG
    CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC
    CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT
    GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC
    TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA
    GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC
    GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA
    GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA
    CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT
    GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG
    ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC
    CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA
    GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG
    AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG
    AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC
    AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC
    AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT
    CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG
    CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC
    CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG
    GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG
    CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC
    AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC
    AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC
    GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG
    GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC
    ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG
    GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA
    ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG
    CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC
    AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC
    CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG
    AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT
    TCTGCACTGG ATCAGTTTGA TGACTCATTT TCTTCCTCTT CATCTTCTTT AGTTGAATTT
    TGGGAAGATG TAAGTTCTCC AGCCATGTTA TTGCAGAAGT GTCTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150649
    Clone ID Related Accession (Same CDS sequence) XM_021468481.1
    Accession Version XM_021468481.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5403bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase MRCK alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGTCGTCT
    CAACCTAACG ATGAGGAGCG CTTCCAGTTC CCACTTCAGG TGACTGATGT GTCCGAGGAT
    GCTAAAGATC TTGTTCGGCG GCTGATCTGC AGCAGAGAAC ACCGGCTGGG CCAGAACGGT
    ATCGAAGACT TTAAGCAGCA CCCCTTTTTC ACAGGCATCG ACTGGGACAA CATCCGCACA
    TGCGAAGCCC CCTACATCCC TGAAGTCAGC AGTCCCACTG ACACCTCCAA CTTTGATGTG
    GATGACGACT GCCTCAAAAA CTGTGAGACG TTGCCTCCAC CTTCACACAC AGCATTTTCT
    GGACACCACC TCCCATTCGT AGGCTTCACC TACACGAGTA AATGCACGCT GTCAGATCGA
    GGGTGTTTGC GGGAATCGGT CGGACCTGCA CAGGTTGATG CTCATGTTCA GCGTAGCCTG
    GAGGAGAGCC TGGCCTCTGA AGCCTATGAG AGGAGAATTC GACGTTTAGA GCAGGAGAAA
    ACCGAACTTA CCCGTAAACT ACAGGAGTCT ACGCAAACGG TGCAAGCACT GCAGTACCCT
    GATTCAGACG CCCCAGTGAA TGCTAATAAA GAGGTAGAGA TAAGGAGTTT AAAGAGTGAG
    ATCGACATCC TGAAGAAACA GATAGCAGAC TCTGGTCAGA TGGAGCAGCA GTTGGAGAAC
    GCCAGCACAG CTAGAAGAGA CCTGGAAGAC TCGTCCAAGG TCGTCAGGAA CTTAGAGAAA
    CAGCTGAAGA GTGTCACACA AGAGAAAGAT GATCTGCACA AGGAGCTGCT GGGTTTGGCA
    GAGAAGGTGA AGGCTCAGGG GAAGGAGCTA AAGGATGCTC ACAGTCAAAA AAAGATGGCC
    ATAAAGGAGT TCGCTGAGCT GAATGAACAG CTGAGCGATG TTCGCACACA AAAGCAGCGC
    CTCAGCCGAC AGTTCAAAGA CAAGGAGGAG GAGATGGAAG GAGTGTCGCA GAAACTAGAG
    GCTCTACGGC TAGAGATACG CAAAGCCGAG AGAATGCGCA AAGAGTTGGA GGCCCAGGCT
    GAGGAACATG CAGCAGAAGC TCAGAAGGAG AGAAAGGTTC GAGAAAGAAC AGAACAGTAC
    AGCAAACAAC TAGAGGAGGA GCTCGAGGGC ATGAAGCAGT TGAAGCAGAC TGGGCCTCCT
    AACGGAGCTG CAAGCTCAGA CCAGCAGCAG GAGCTGATGC GCGTTCGGGC TGAACTGGAG
    AAGAAGAGCG TACAGTACGA GGAGGAGATG TGTCGCAGAG AGACGCTCCA CAGCAGTGAG
    ATGAAGAACC TCCAAAAAGA GCTACGGGAT GCCGAGGGTC AACACCTCAC ACATCAGAAA
    GAGATACTCG TGCTTAAAGA CAAACTGGAG AAAACTCGCA GAGAAAGTCA AGTCGAGCGA
    GAAGAATTTG TGTGCGAGTA CAAGCAGAAG TACGAGAGGG AAAAAGCCCA TCTGACAGAC
    GAAAACAAGA AGCTGAGTGC CGAAGTGGAG AAGTTGAACG GTCAGTTCGA GCAGCTGAGC
    AGCAGTCACC GGCAGCTGGA GGAGGAGATG AGAGAACTGG CTGATAAGAA GGAGAGTGTT
    GCCCACTGGG AGGCCCAGAT CACCGAGATC ATCCAGTGGG TGAGCGATGA GAAGGATGCC
    CGTGGTTACC TTCAAGCTCT GGCCACCAAA ATGACAGAGG AGCTGGAAGG GCTCAGAAAC
    ACCAGTTTGG GAGCCAGAGG AACGGACATG CCGTGGAAGA TGAGGCGTTT CGCCAAGCTG
    GACATGTCTG CGCGTCTGGA GCTGCAGTCG GCGCTGGATA CTGAGATCAG AGCCAAACAG
    TCCATCCAAG ACGAGCTTAA CAAAGTCAAA GCAACCAACA TTGCCACAGA ATGCAAATTG
    CAAGAATCCG AAAGTAGAAA CCAGGAGCTG CTGGGAGAAA TTGAGAGACT GAGGAAGGAG
    ACAGAGGAGC TAAGGCTGAG GAGAGGCGTG AAGCACCAGG ATTCCCAAAA TTCCTTTTTG
    GCTTTCCTCA ATGCGCCCAC TTCTGCACTG GATCAGTTTG ATCGCTCACC CTCTTTTGGT
    CCCGGAAGCA AAACCAGACG TCTTGACTCT GCAGATTTCA CCCCTTCCAA CACTCCATCC
    AGAGATGAAG ACGCAAGATC TCGCCGTATT TCACGCTCTC GCTCACCCTC CACCGCCAGT
    GACATGGAGC CTATAGAGTT GATTGACCAC ACATCACGTA GTCTTCAGAC CCCAACAGGC
    AGATCAGGCT ACGGGAGCTT GGGACACTCA TCACCTAAGC CCAAGGCACA CCAGTTTAGT
    GTAAAGTCCT TCAATGTTCC CACTAAATGT AACCAGTGCA CCTCTCTCAT GGTGGGACTC
    ATCCGTCAGG GCTGCACATG CGAAGCCTGC AGCTTCTCCT GTCATGTCAC TTGTGCTGAT
    AAAGCCCCTT CTGTGTGCCC CGTCGCACCA GACCAGACCA GGGGCAAACT GGGCATTGAC
    ACTCAGAGGG GCATTGGGAC TGCATATGAG GGGCATCTTA GGGTGCCGAA GCCCACTGGG
    GTGAAGAAGG GTTGGCAGAG GGTCTGGGCA GTGGTTTGTG ACTTCAAACT TTTTCTGTAT
    GAGCTTGGAG AAGGGAAAGG TGCTCAGCCT GGTGTTGTAG TAAACCAGGT CATTGACATG
    AGGGATGAAG AGTTTTCCGC CAGTTCGGTT CTGGAGTCTG ATGTCATCCA TGCTAATAGG
    AAGGATATAC CCTGTATTTT TAGGGTAACA GCATCTCAGC TGTCCGCCTC CTCCAGTCAG
    AAGACGTGTA TTCTAATTCT GGCTGACAAC GAGCAAGAAA GGACGAAGTG GGTGTGCGCT
    CTGAATGAAC TGCATCGCAT CCTGAGGAAA AACAAGCTGA AGGAGCGTTT CGTCTATGTG
    CCTAAAGAAG CCTACGACAG CACACTTCCT CTCATCAAAA CCACCCAAAC TGCTGCTATT
    ATAGATCATG AACGCGTTGC TTTGGGCAAT GAGGAGGGCC TGTTTGTCAT CCACGTCACC
    AAAGATGAAA TCATTCGTGT GGGTGATGTC AAGAAGGTGC ATCATGTAGA ACTGATGCCC
    ACGGAGCACA TACTGGCCGT CATCTCGGGC AGGAACCGAC AAGTATGTCT GGTCTCCATG
    GCGGGTCTCG ACGGGAGGGA GGTCGACAAG AATAAGGTGG CAGATACTAA AAACTGCCAG
    TTGCTGGTGT CCGGTGTGGT TCGCAATGTC ACCTGCTTCT GCCTCGCCAT TAAACGGCAG
    ATCTCATGCT TCGAAATTAA CAAGAGCAAG TCACGACACT CCCATCTCGT GGACATACAA
    GCTCCAGGAA CTGTCCAATG GATGGCGCTG TTCAACCAGC AGTTGTGTGT TGGTTACCCA
    TCAGGCTTCA TGCGCTACAG TTTGTATGGA GAAACACCTC CAGCCAGCTT ACTTCATCCA
    GATGACCCCA CACTGGCATT TATTAAAACA GACAACCTGG ATGCCCTTTG TGCTGTGGAA
    ATCTCCAATA AGGAACTGCT GCTCTGCTTC AGTAAAATAG CAGTGTATGT TGACACACAC
    GGCAGACGCT CGCGCCAGCA GGAGCTGATG TGGCCCGCGG CGCCCACCGC CTGCTGCTAT
    AACGCCCCCT ATCTGTCGGT CTACAGTGAA AATGCAGTGG ATGTGTTTGA TGTGAACTCT
    ACGGAATGGA TTCAGACCAT TCCTCTCAAA AAGGTCCGGC CATTAAACAT GGATGGCTCT
    TTGAATCTCC TGGGCCTAGA AACGGTCAGA CTCATCTACT TCAGAAACAA GACCGCAGAG
    GGGGATGAAC TGGTGGTGCC TGAAGTGTGT GATAACAGCA GAAAGCAGAT GGTGCGAAGC
    ATGAGCAACA AGAGACGATT CTCGTTTCGT ATTCCTGAGG AGGAGAGACT GCAGCAGAGG
    AGAGAAATGT TGAAAGATCC TGAGAGGAGG AACAAGCTGA TCTCAAATCC TACAAATTTT
    AACCATGTGG CTCATATGGG ACCTGGAGAT GGCATGCAGA TACTCAGAGA CCTGCCAATG
    AATGTACGTG CTCAGGACAA CCGTGCTGGA TTCAGCGGCT CCGTCAGCAT CCCCTCCATT
    ACAAAAAACA GAGCAGAACC AGGGCGATCC ATGAGCGCAA GCAGTGGCCT AGGAATGCGG
    TCGTCCTCTC AGAACGGCAG TGCTCTAAGG AGGGAGCTGT CCAGCGGCAG CTACAGCTCT
    AAACGGCAGA CCATGACTTC ACCCTCCGAG AGTTCGCTGT CATCAGGAGG AGGGATGGAC
    GTGGGGGAAG CGCCGCTCTC TCAGTTTGAC AGAGAGTGGC AGGCAGTATC GCCCTCGGAG
    AAGACCCCTG ATCTGAAAAG GGCTTTAAGG ACCCTGCTTA GTAAGATGAA GGACTCTGAT
    TCGCCGCGGC ACTCCACTGC CTCCAACAGC TCCACCTTCA GCAGCCCGCC GAGCCCCGCC
    TCCCCACACA AGACAAAGTC CCTCTCATTG GAGAGCACAG ACCGCACCGC CTGGGACACA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324156.1
    CDS1641..7043
    Translation

    Target ORF information:

    RefSeq Version XM_021468481.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468481.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGTCGTCT
    CAACCTAACG ATGAGGAGCG CTTCCAGTTC CCACTTCAGG TGACTGATGT GTCCGAGGAT
    GCTAAAGATC TTGTTCGGCG GCTGATCTGC AGCAGAGAAC ACCGGCTGGG CCAGAACGGT
    ATCGAAGACT TTAAGCAGCA CCCCTTTTTC ACAGGCATCG ACTGGGACAA CATCCGCACA
    TGCGAAGCCC CCTACATCCC TGAAGTCAGC AGTCCCACTG ACACCTCCAA CTTTGATGTG
    GATGACGACT GCCTCAAAAA CTGTGAGACG TTGCCTCCAC CTTCACACAC AGCATTTTCT
    GGACACCACC TCCCATTCGT AGGCTTCACC TACACGAGTA AATGCACGCT GTCAGATCGA
    GGGTGTTTGC GGGAATCGGT CGGACCTGCA CAGGTTGATG CTCATGTTCA GCGTAGCCTG
    GAGGAGAGCC TGGCCTCTGA AGCCTATGAG AGGAGAATTC GACGTTTAGA GCAGGAGAAA
    ACCGAACTTA CCCGTAAACT ACAGGAGTCT ACGCAAACGG TGCAAGCACT GCAGTACCCT
    GATTCAGACG CCCCAGTGAA TGCTAATAAA GAGGTAGAGA TAAGGAGTTT AAAGAGTGAG
    ATCGACATCC TGAAGAAACA GATAGCAGAC TCTGGTCAGA TGGAGCAGCA GTTGGAGAAC
    GCCAGCACAG CTAGAAGAGA CCTGGAAGAC TCGTCCAAGG TCGTCAGGAA CTTAGAGAAA
    CAGCTGAAGA GTGTCACACA AGAGAAAGAT GATCTGCACA AGGAGCTGCT GGGTTTGGCA
    GAGAAGGTGA AGGCTCAGGG GAAGGAGCTA AAGGATGCTC ACAGTCAAAA AAAGATGGCC
    ATAAAGGAGT TCGCTGAGCT GAATGAACAG CTGAGCGATG TTCGCACACA AAAGCAGCGC
    CTCAGCCGAC AGTTCAAAGA CAAGGAGGAG GAGATGGAAG GAGTGTCGCA GAAACTAGAG
    GCTCTACGGC TAGAGATACG CAAAGCCGAG AGAATGCGCA AAGAGTTGGA GGCCCAGGCT
    GAGGAACATG CAGCAGAAGC TCAGAAGGAG AGAAAGGTTC GAGAAAGAAC AGAACAGTAC
    AGCAAACAAC TAGAGGAGGA GCTCGAGGGC ATGAAGCAGT TGAAGCAGAC TGGGCCTCCT
    AACGGAGCTG CAAGCTCAGA CCAGCAGCAG GAGCTGATGC GCGTTCGGGC TGAACTGGAG
    AAGAAGAGCG TACAGTACGA GGAGGAGATG TGTCGCAGAG AGACGCTCCA CAGCAGTGAG
    ATGAAGAACC TCCAAAAAGA GCTACGGGAT GCCGAGGGTC AACACCTCAC ACATCAGAAA
    GAGATACTCG TGCTTAAAGA CAAACTGGAG AAAACTCGCA GAGAAAGTCA AGTCGAGCGA
    GAAGAATTTG TGTGCGAGTA CAAGCAGAAG TACGAGAGGG AAAAAGCCCA TCTGACAGAC
    GAAAACAAGA AGCTGAGTGC CGAAGTGGAG AAGTTGAACG GTCAGTTCGA GCAGCTGAGC
    AGCAGTCACC GGCAGCTGGA GGAGGAGATG AGAGAACTGG CTGATAAGAA GGAGAGTGTT
    GCCCACTGGG AGGCCCAGAT CACCGAGATC ATCCAGTGGG TGAGCGATGA GAAGGATGCC
    CGTGGTTACC TTCAAGCTCT GGCCACCAAA ATGACAGAGG AGCTGGAAGG GCTCAGAAAC
    ACCAGTTTGG GAGCCAGAGG AACGGACATG CCGTGGAAGA TGAGGCGTTT CGCCAAGCTG
    GACATGTCTG CGCGTCTGGA GCTGCAGTCG GCGCTGGATA CTGAGATCAG AGCCAAACAG
    TCCATCCAAG ACGAGCTTAA CAAAGTCAAA GCAACCAACA TTGCCACAGA ATGCAAATTG
    CAAGAATCCG AAAGTAGAAA CCAGGAGCTG CTGGGAGAAA TTGAGAGACT GAGGAAGGAG
    ACAGAGGAGC TAAGGCTGAG GAGAGGCGTG AAGCACCAGG ATTCCCAAAA TTCCTTTTTG
    GCTTTCCTCA ATGCGCCCAC TTCTGCACTG GATCAGTTTG ATCGCTCACC CTCTTTTGGT
    CCCGGAAGCA AAACCAGACG TCTTGACTCT GCAGATTTCA CCCCTTCCAA CACTCCATCC
    AGAGATGAAG ACGCAAGATC TCGCCGTATT TCACGCTCTC GCTCACCCTC CACCGCCAGT
    GACATGGAGC CTATAGAGTT GATTGACCAC ACATCACGTA GTCTTCAGAC CCCAACAGGC
    AGATCAGGCT ACGGGAGCTT GGGACACTCA TCACCTAAGC CCAAGGCACA CCAGTTTAGT
    GTAAAGTCCT TCAATGTTCC CACTAAATGT AACCAGTGCA CCTCTCTCAT GGTGGGACTC
    ATCCGTCAGG GCTGCACATG CGAAGCCTGC AGCTTCTCCT GTCATGTCAC TTGTGCTGAT
    AAAGCCCCTT CTGTGTGCCC CGTCGCACCA GACCAGACCA GGGGCAAACT GGGCATTGAC
    ACTCAGAGGG GCATTGGGAC TGCATATGAG GGGCATCTTA GGGTGCCGAA GCCCACTGGG
    GTGAAGAAGG GTTGGCAGAG GGTCTGGGCA GTGGTTTGTG ACTTCAAACT TTTTCTGTAT
    GAGCTTGGAG AAGGGAAAGG TGCTCAGCCT GGTGTTGTAG TAAACCAGGT CATTGACATG
    AGGGATGAAG AGTTTTCCGC CAGTTCGGTT CTGGAGTCTG ATGTCATCCA TGCTAATAGG
    AAGGATATAC CCTGTATTTT TAGGGTAACA GCATCTCAGC TGTCCGCCTC CTCCAGTCAG
    AAGACGTGTA TTCTAATTCT GGCTGACAAC GAGCAAGAAA GGACGAAGTG GGTGTGCGCT
    CTGAATGAAC TGCATCGCAT CCTGAGGAAA AACAAGCTGA AGGAGCGTTT CGTCTATGTG
    CCTAAAGAAG CCTACGACAG CACACTTCCT CTCATCAAAA CCACCCAAAC TGCTGCTATT
    ATAGATCATG AACGCGTTGC TTTGGGCAAT GAGGAGGGCC TGTTTGTCAT CCACGTCACC
    AAAGATGAAA TCATTCGTGT GGGTGATGTC AAGAAGGTGC ATCATGTAGA ACTGATGCCC
    ACGGAGCACA TACTGGCCGT CATCTCGGGC AGGAACCGAC AAGTATGTCT GGTCTCCATG
    GCGGGTCTCG ACGGGAGGGA GGTCGACAAG AATAAGGTGG CAGATACTAA AAACTGCCAG
    TTGCTGGTGT CCGGTGTGGT TCGCAATGTC ACCTGCTTCT GCCTCGCCAT TAAACGGCAG
    ATCTCATGCT TCGAAATTAA CAAGAGCAAG TCACGACACT CCCATCTCGT GGACATACAA
    GCTCCAGGAA CTGTCCAATG GATGGCGCTG TTCAACCAGC AGTTGTGTGT TGGTTACCCA
    TCAGGCTTCA TGCGCTACAG TTTGTATGGA GAAACACCTC CAGCCAGCTT ACTTCATCCA
    GATGACCCCA CACTGGCATT TATTAAAACA GACAACCTGG ATGCCCTTTG TGCTGTGGAA
    ATCTCCAATA AGGAACTGCT GCTCTGCTTC AGTAAAATAG CAGTGTATGT TGACACACAC
    GGCAGACGCT CGCGCCAGCA GGAGCTGATG TGGCCCGCGG CGCCCACCGC CTGCTGCTAT
    AACGCCCCCT ATCTGTCGGT CTACAGTGAA AATGCAGTGG ATGTGTTTGA TGTGAACTCT
    ACGGAATGGA TTCAGACCAT TCCTCTCAAA AAGGTCCGGC CATTAAACAT GGATGGCTCT
    TTGAATCTCC TGGGCCTAGA AACGGTCAGA CTCATCTACT TCAGAAACAA GACCGCAGAG
    GGGGATGAAC TGGTGGTGCC TGAAGTGTGT GATAACAGCA GAAAGCAGAT GGTGCGAAGC
    ATGAGCAACA AGAGACGATT CTCGTTTCGT ATTCCTGAGG AGGAGAGACT GCAGCAGAGG
    AGAGAAATGT TGAAAGATCC TGAGAGGAGG AACAAGCTGA TCTCAAATCC TACAAATTTT
    AACCATGTGG CTCATATGGG ACCTGGAGAT GGCATGCAGA TACTCAGAGA CCTGCCAATG
    AATGTACGTG CTCAGGACAA CCGTGCTGGA TTCAGCGGCT CCGTCAGCAT CCCCTCCATT
    ACAAAAAACA GAGCAGAACC AGGGCGATCC ATGAGCGCAA GCAGTGGCCT AGGAATGCGG
    TCGTCCTCTC AGAACGGCAG TGCTCTAAGG AGGGAGCTGT CCAGCGGCAG CTACAGCTCT
    AAACGGCAGA CCATGACTTC ACCCTCCGAG AGTTCGCTGT CATCAGGAGG AGGGATGGAC
    GTGGGGGAAG CGCCGCTCTC TCAGTTTGAC AGAGAGTGGC AGGCAGTATC GCCCTCGGAG
    AAGACCCCTG ATCTGAAAAG GGCTTTAAGG ACCCTGCTTA GTAAGATGAA GGACTCTGAT
    TCGCCGCGGC ACTCCACTGC CTCCAACAGC TCCACCTTCA GCAGCCCGCC GAGCCCCGCC
    TCCCCACACA AGACAAAGTC CCTCTCATTG GAGAGCACAG ACCGCACCGC CTGGGACACA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150650
    Clone ID Related Accession (Same CDS sequence) XM_021468482.1
    Accession Version XM_021468482.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5400bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase MRCK alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGTCGTCT
    CAACCTAACG ATGAGGAGCG CTTCCAGTTC CCACTTCAGG TGACTGATGT GTCCGAGGAT
    GCTAAAGATC TTGTTCGGCG GCTGATCTGC AGCAGAGAAC ACCGGCTGGG CCAGAACGGT
    ATCGAAGACT TTAAGCAGCA CCCCTTTTTC ACAGGCATCG ACTGGGACAA CATCCGCACA
    TGCGAAGCCC CCTACATCCC TGAAGTCAGC AGTCCCACTG ACACCTCCAA CTTTGATGTG
    GATGACGACT GCCTCAAAAA CTGTGAGACG TTGCCTCCAC CTTCACACAC AGCATTTTCT
    GGACACCACC TCCCATTCGT AGGCTTCACC TACACGAGTA AATGCACGCT GTCAGATCGA
    GGGTGTTTGC GGGAATCGGT CGGACCTGCA CAGGTTGATG CTCATGTTCA GCGTAGCCTG
    GAGGAGAGCC TGGCCTCTGA AGCCTATGAG AGGAGAATTC GACGTTTAGA GCAGGAGAAA
    ACCGAACTTA CCCGTAAACT ACAGGAGTCT ACGCAAACGG TGCAAGCACT GCAGTACCCT
    GATTCAGACG CCCCAGTGAA TGCTAATAAA GAGGTAGAGA TAAGGAGTTT AAAGAGTGAG
    ATCGACATCC TGAAGAAACA GATAGCAGAC TCTGGTCAGA TGGAGCAGCA GTTGGAGAAC
    GCCAGCACAG CTAGAAGAGA CCTGGAAGAC TCGTCCAAGG TCGTCAGGAA CTTAGAGAAA
    CAGCTGAAGA GTGTCACACA AGAGAAAGAT GATCTGCACA AGGAGCTGCT GGGTTTGGCA
    GAGAAGGTGA AGGCTCAGGG GAAGGAGCTA AAGGATGCTC ACAGTCAAAA AAAGATGGCC
    ATAAAGGAGT TCGCTGAGCT GAATGAACAG CTGAGCGATG TTCGCACACA AAAGCAGCGC
    CTCAGCCGAC AGTTCAAAGA CAAGGAGGAG GAGATGGAAG GAGTGTCGCA GAAACTAGAG
    GCTCTACGGC TAGAGATACG CAAAGCCGAG AGAATGCGCA AAGAGTTGGA GGCCCAGGCT
    GAGGAACATG CAGCAGAAGC TCAGAAGGAG AGAAAGGTTC GAGAAAGAAC AGAACAGTAC
    AGCAAACAAC TAGAGGAGGA GCTCGAGGGC ATGAAGTTGA AGCAGACTGG GCCTCCTAAC
    GGAGCTGCAA GCTCAGACCA GCAGCAGGAG CTGATGCGCG TTCGGGCTGA ACTGGAGAAG
    AAGAGCGTAC AGTACGAGGA GGAGATGTGT CGCAGAGAGA CGCTCCACAG CAGTGAGATG
    AAGAACCTCC AAAAAGAGCT ACGGGATGCC GAGGGTCAAC ACCTCACACA TCAGAAAGAG
    ATACTCGTGC TTAAAGACAA ACTGGAGAAA ACTCGCAGAG AAAGTCAAGT CGAGCGAGAA
    GAATTTGTGT GCGAGTACAA GCAGAAGTAC GAGAGGGAAA AAGCCCATCT GACAGACGAA
    AACAAGAAGC TGAGTGCCGA AGTGGAGAAG TTGAACGGTC AGTTCGAGCA GCTGAGCAGC
    AGTCACCGGC AGCTGGAGGA GGAGATGAGA GAACTGGCTG ATAAGAAGGA GAGTGTTGCC
    CACTGGGAGG CCCAGATCAC CGAGATCATC CAGTGGGTGA GCGATGAGAA GGATGCCCGT
    GGTTACCTTC AAGCTCTGGC CACCAAAATG ACAGAGGAGC TGGAAGGGCT CAGAAACACC
    AGTTTGGGAG CCAGAGGAAC GGACATGCCG TGGAAGATGA GGCGTTTCGC CAAGCTGGAC
    ATGTCTGCGC GTCTGGAGCT GCAGTCGGCG CTGGATACTG AGATCAGAGC CAAACAGTCC
    ATCCAAGACG AGCTTAACAA AGTCAAAGCA ACCAACATTG CCACAGAATG CAAATTGCAA
    GAATCCGAAA GTAGAAACCA GGAGCTGCTG GGAGAAATTG AGAGACTGAG GAAGGAGACA
    GAGGAGCTAA GGCTGAGGAG AGGCGTGAAG CACCAGGATT CCCAAAATTC CTTTTTGGCT
    TTCCTCAATG CGCCCACTTC TGCACTGGAT CAGTTTGATC GCTCACCCTC TTTTGGTCCC
    GGAAGCAAAA CCAGACGTCT TGACTCTGCA GATTTCACCC CTTCCAACAC TCCATCCAGA
    GATGAAGACG CAAGATCTCG CCGTATTTCA CGCTCTCGCT CACCCTCCAC CGCCAGTGAC
    ATGGAGCCTA TAGAGTTGAT TGACCACACA TCACGTAGTC TTCAGACCCC AACAGGCAGA
    TCAGGCTACG GGAGCTTGGG ACACTCATCA CCTAAGCCCA AGGCACACCA GTTTAGTGTA
    AAGTCCTTCA ATGTTCCCAC TAAATGTAAC CAGTGCACCT CTCTCATGGT GGGACTCATC
    CGTCAGGGCT GCACATGCGA AGCCTGCAGC TTCTCCTGTC ATGTCACTTG TGCTGATAAA
    GCCCCTTCTG TGTGCCCCGT CGCACCAGAC CAGACCAGGG GCAAACTGGG CATTGACACT
    CAGAGGGGCA TTGGGACTGC ATATGAGGGG CATCTTAGGG TGCCGAAGCC CACTGGGGTG
    AAGAAGGGTT GGCAGAGGGT CTGGGCAGTG GTTTGTGACT TCAAACTTTT TCTGTATGAG
    CTTGGAGAAG GGAAAGGTGC TCAGCCTGGT GTTGTAGTAA ACCAGGTCAT TGACATGAGG
    GATGAAGAGT TTTCCGCCAG TTCGGTTCTG GAGTCTGATG TCATCCATGC TAATAGGAAG
    GATATACCCT GTATTTTTAG GGTAACAGCA TCTCAGCTGT CCGCCTCCTC CAGTCAGAAG
    ACGTGTATTC TAATTCTGGC TGACAACGAG CAAGAAAGGA CGAAGTGGGT GTGCGCTCTG
    AATGAACTGC ATCGCATCCT GAGGAAAAAC AAGCTGAAGG AGCGTTTCGT CTATGTGCCT
    AAAGAAGCCT ACGACAGCAC ACTTCCTCTC ATCAAAACCA CCCAAACTGC TGCTATTATA
    GATCATGAAC GCGTTGCTTT GGGCAATGAG GAGGGCCTGT TTGTCATCCA CGTCACCAAA
    GATGAAATCA TTCGTGTGGG TGATGTCAAG AAGGTGCATC ATGTAGAACT GATGCCCACG
    GAGCACATAC TGGCCGTCAT CTCGGGCAGG AACCGACAAG TATGTCTGGT CTCCATGGCG
    GGTCTCGACG GGAGGGAGGT CGACAAGAAT AAGGTGGCAG ATACTAAAAA CTGCCAGTTG
    CTGGTGTCCG GTGTGGTTCG CAATGTCACC TGCTTCTGCC TCGCCATTAA ACGGCAGATC
    TCATGCTTCG AAATTAACAA GAGCAAGTCA CGACACTCCC ATCTCGTGGA CATACAAGCT
    CCAGGAACTG TCCAATGGAT GGCGCTGTTC AACCAGCAGT TGTGTGTTGG TTACCCATCA
    GGCTTCATGC GCTACAGTTT GTATGGAGAA ACACCTCCAG CCAGCTTACT TCATCCAGAT
    GACCCCACAC TGGCATTTAT TAAAACAGAC AACCTGGATG CCCTTTGTGC TGTGGAAATC
    TCCAATAAGG AACTGCTGCT CTGCTTCAGT AAAATAGCAG TGTATGTTGA CACACACGGC
    AGACGCTCGC GCCAGCAGGA GCTGATGTGG CCCGCGGCGC CCACCGCCTG CTGCTATAAC
    GCCCCCTATC TGTCGGTCTA CAGTGAAAAT GCAGTGGATG TGTTTGATGT GAACTCTACG
    GAATGGATTC AGACCATTCC TCTCAAAAAG GTCCGGCCAT TAAACATGGA TGGCTCTTTG
    AATCTCCTGG GCCTAGAAAC GGTCAGACTC ATCTACTTCA GAAACAAGAC CGCAGAGGGG
    GATGAACTGG TGGTGCCTGA AGTGTGTGAT AACAGCAGAA AGCAGATGGT GCGAAGCATG
    AGCAACAAGA GACGATTCTC GTTTCGTATT CCTGAGGAGG AGAGACTGCA GCAGAGGAGA
    GAAATGTTGA AAGATCCTGA GAGGAGGAAC AAGCTGATCT CAAATCCTAC AAATTTTAAC
    CATGTGGCTC ATATGGGACC TGGAGATGGC ATGCAGATAC TCAGAGACCT GCCAATGAAT
    GTACGTGCTC AGGACAACCG TGCTGGATTC AGCGGCTCCG TCAGCATCCC CTCCATTACA
    AAAAACAGAG CAGAACCAGG GCGATCCATG AGCGCAAGCA GTGGCCTAGG AATGCGGTCG
    TCCTCTCAGA ACGGCAGTGC TCTAAGGAGG GAGCTGTCCA GCGGCAGCTA CAGCTCTAAA
    CGGCAGACCA TGACTTCACC CTCCGAGAGT TCGCTGTCAT CAGGAGGAGG GATGGACGTG
    GGGGAAGCGC CGCTCTCTCA GTTTGACAGA GAGTGGCAGG CAGTATCGCC CTCGGAGAAG
    ACCCCTGATC TGAAAAGGGC TTTAAGGACC CTGCTTAGTA AGATGAAGGA CTCTGATTCG
    CCGCGGCACT CCACTGCCTC CAACAGCTCC ACCTTCAGCA GCCCGCCGAG CCCCGCCTCC
    CCACACAAGA CAAAGTCCCT CTCATTGGAG AGCACAGACC GCACCGCCTG GGACACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324157.1
    CDS1641..7040
    Translation

    Target ORF information:

    RefSeq Version XM_021468482.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468482.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGTCGTCT
    CAACCTAACG ATGAGGAGCG CTTCCAGTTC CCACTTCAGG TGACTGATGT GTCCGAGGAT
    GCTAAAGATC TTGTTCGGCG GCTGATCTGC AGCAGAGAAC ACCGGCTGGG CCAGAACGGT
    ATCGAAGACT TTAAGCAGCA CCCCTTTTTC ACAGGCATCG ACTGGGACAA CATCCGCACA
    TGCGAAGCCC CCTACATCCC TGAAGTCAGC AGTCCCACTG ACACCTCCAA CTTTGATGTG
    GATGACGACT GCCTCAAAAA CTGTGAGACG TTGCCTCCAC CTTCACACAC AGCATTTTCT
    GGACACCACC TCCCATTCGT AGGCTTCACC TACACGAGTA AATGCACGCT GTCAGATCGA
    GGGTGTTTGC GGGAATCGGT CGGACCTGCA CAGGTTGATG CTCATGTTCA GCGTAGCCTG
    GAGGAGAGCC TGGCCTCTGA AGCCTATGAG AGGAGAATTC GACGTTTAGA GCAGGAGAAA
    ACCGAACTTA CCCGTAAACT ACAGGAGTCT ACGCAAACGG TGCAAGCACT GCAGTACCCT
    GATTCAGACG CCCCAGTGAA TGCTAATAAA GAGGTAGAGA TAAGGAGTTT AAAGAGTGAG
    ATCGACATCC TGAAGAAACA GATAGCAGAC TCTGGTCAGA TGGAGCAGCA GTTGGAGAAC
    GCCAGCACAG CTAGAAGAGA CCTGGAAGAC TCGTCCAAGG TCGTCAGGAA CTTAGAGAAA
    CAGCTGAAGA GTGTCACACA AGAGAAAGAT GATCTGCACA AGGAGCTGCT GGGTTTGGCA
    GAGAAGGTGA AGGCTCAGGG GAAGGAGCTA AAGGATGCTC ACAGTCAAAA AAAGATGGCC
    ATAAAGGAGT TCGCTGAGCT GAATGAACAG CTGAGCGATG TTCGCACACA AAAGCAGCGC
    CTCAGCCGAC AGTTCAAAGA CAAGGAGGAG GAGATGGAAG GAGTGTCGCA GAAACTAGAG
    GCTCTACGGC TAGAGATACG CAAAGCCGAG AGAATGCGCA AAGAGTTGGA GGCCCAGGCT
    GAGGAACATG CAGCAGAAGC TCAGAAGGAG AGAAAGGTTC GAGAAAGAAC AGAACAGTAC
    AGCAAACAAC TAGAGGAGGA GCTCGAGGGC ATGAAGTTGA AGCAGACTGG GCCTCCTAAC
    GGAGCTGCAA GCTCAGACCA GCAGCAGGAG CTGATGCGCG TTCGGGCTGA ACTGGAGAAG
    AAGAGCGTAC AGTACGAGGA GGAGATGTGT CGCAGAGAGA CGCTCCACAG CAGTGAGATG
    AAGAACCTCC AAAAAGAGCT ACGGGATGCC GAGGGTCAAC ACCTCACACA TCAGAAAGAG
    ATACTCGTGC TTAAAGACAA ACTGGAGAAA ACTCGCAGAG AAAGTCAAGT CGAGCGAGAA
    GAATTTGTGT GCGAGTACAA GCAGAAGTAC GAGAGGGAAA AAGCCCATCT GACAGACGAA
    AACAAGAAGC TGAGTGCCGA AGTGGAGAAG TTGAACGGTC AGTTCGAGCA GCTGAGCAGC
    AGTCACCGGC AGCTGGAGGA GGAGATGAGA GAACTGGCTG ATAAGAAGGA GAGTGTTGCC
    CACTGGGAGG CCCAGATCAC CGAGATCATC CAGTGGGTGA GCGATGAGAA GGATGCCCGT
    GGTTACCTTC AAGCTCTGGC CACCAAAATG ACAGAGGAGC TGGAAGGGCT CAGAAACACC
    AGTTTGGGAG CCAGAGGAAC GGACATGCCG TGGAAGATGA GGCGTTTCGC CAAGCTGGAC
    ATGTCTGCGC GTCTGGAGCT GCAGTCGGCG CTGGATACTG AGATCAGAGC CAAACAGTCC
    ATCCAAGACG AGCTTAACAA AGTCAAAGCA ACCAACATTG CCACAGAATG CAAATTGCAA
    GAATCCGAAA GTAGAAACCA GGAGCTGCTG GGAGAAATTG AGAGACTGAG GAAGGAGACA
    GAGGAGCTAA GGCTGAGGAG AGGCGTGAAG CACCAGGATT CCCAAAATTC CTTTTTGGCT
    TTCCTCAATG CGCCCACTTC TGCACTGGAT CAGTTTGATC GCTCACCCTC TTTTGGTCCC
    GGAAGCAAAA CCAGACGTCT TGACTCTGCA GATTTCACCC CTTCCAACAC TCCATCCAGA
    GATGAAGACG CAAGATCTCG CCGTATTTCA CGCTCTCGCT CACCCTCCAC CGCCAGTGAC
    ATGGAGCCTA TAGAGTTGAT TGACCACACA TCACGTAGTC TTCAGACCCC AACAGGCAGA
    TCAGGCTACG GGAGCTTGGG ACACTCATCA CCTAAGCCCA AGGCACACCA GTTTAGTGTA
    AAGTCCTTCA ATGTTCCCAC TAAATGTAAC CAGTGCACCT CTCTCATGGT GGGACTCATC
    CGTCAGGGCT GCACATGCGA AGCCTGCAGC TTCTCCTGTC ATGTCACTTG TGCTGATAAA
    GCCCCTTCTG TGTGCCCCGT CGCACCAGAC CAGACCAGGG GCAAACTGGG CATTGACACT
    CAGAGGGGCA TTGGGACTGC ATATGAGGGG CATCTTAGGG TGCCGAAGCC CACTGGGGTG
    AAGAAGGGTT GGCAGAGGGT CTGGGCAGTG GTTTGTGACT TCAAACTTTT TCTGTATGAG
    CTTGGAGAAG GGAAAGGTGC TCAGCCTGGT GTTGTAGTAA ACCAGGTCAT TGACATGAGG
    GATGAAGAGT TTTCCGCCAG TTCGGTTCTG GAGTCTGATG TCATCCATGC TAATAGGAAG
    GATATACCCT GTATTTTTAG GGTAACAGCA TCTCAGCTGT CCGCCTCCTC CAGTCAGAAG
    ACGTGTATTC TAATTCTGGC TGACAACGAG CAAGAAAGGA CGAAGTGGGT GTGCGCTCTG
    AATGAACTGC ATCGCATCCT GAGGAAAAAC AAGCTGAAGG AGCGTTTCGT CTATGTGCCT
    AAAGAAGCCT ACGACAGCAC ACTTCCTCTC ATCAAAACCA CCCAAACTGC TGCTATTATA
    GATCATGAAC GCGTTGCTTT GGGCAATGAG GAGGGCCTGT TTGTCATCCA CGTCACCAAA
    GATGAAATCA TTCGTGTGGG TGATGTCAAG AAGGTGCATC ATGTAGAACT GATGCCCACG
    GAGCACATAC TGGCCGTCAT CTCGGGCAGG AACCGACAAG TATGTCTGGT CTCCATGGCG
    GGTCTCGACG GGAGGGAGGT CGACAAGAAT AAGGTGGCAG ATACTAAAAA CTGCCAGTTG
    CTGGTGTCCG GTGTGGTTCG CAATGTCACC TGCTTCTGCC TCGCCATTAA ACGGCAGATC
    TCATGCTTCG AAATTAACAA GAGCAAGTCA CGACACTCCC ATCTCGTGGA CATACAAGCT
    CCAGGAACTG TCCAATGGAT GGCGCTGTTC AACCAGCAGT TGTGTGTTGG TTACCCATCA
    GGCTTCATGC GCTACAGTTT GTATGGAGAA ACACCTCCAG CCAGCTTACT TCATCCAGAT
    GACCCCACAC TGGCATTTAT TAAAACAGAC AACCTGGATG CCCTTTGTGC TGTGGAAATC
    TCCAATAAGG AACTGCTGCT CTGCTTCAGT AAAATAGCAG TGTATGTTGA CACACACGGC
    AGACGCTCGC GCCAGCAGGA GCTGATGTGG CCCGCGGCGC CCACCGCCTG CTGCTATAAC
    GCCCCCTATC TGTCGGTCTA CAGTGAAAAT GCAGTGGATG TGTTTGATGT GAACTCTACG
    GAATGGATTC AGACCATTCC TCTCAAAAAG GTCCGGCCAT TAAACATGGA TGGCTCTTTG
    AATCTCCTGG GCCTAGAAAC GGTCAGACTC ATCTACTTCA GAAACAAGAC CGCAGAGGGG
    GATGAACTGG TGGTGCCTGA AGTGTGTGAT AACAGCAGAA AGCAGATGGT GCGAAGCATG
    AGCAACAAGA GACGATTCTC GTTTCGTATT CCTGAGGAGG AGAGACTGCA GCAGAGGAGA
    GAAATGTTGA AAGATCCTGA GAGGAGGAAC AAGCTGATCT CAAATCCTAC AAATTTTAAC
    CATGTGGCTC ATATGGGACC TGGAGATGGC ATGCAGATAC TCAGAGACCT GCCAATGAAT
    GTACGTGCTC AGGACAACCG TGCTGGATTC AGCGGCTCCG TCAGCATCCC CTCCATTACA
    AAAAACAGAG CAGAACCAGG GCGATCCATG AGCGCAAGCA GTGGCCTAGG AATGCGGTCG
    TCCTCTCAGA ACGGCAGTGC TCTAAGGAGG GAGCTGTCCA GCGGCAGCTA CAGCTCTAAA
    CGGCAGACCA TGACTTCACC CTCCGAGAGT TCGCTGTCAT CAGGAGGAGG GATGGACGTG
    GGGGAAGCGC CGCTCTCTCA GTTTGACAGA GAGTGGCAGG CAGTATCGCC CTCGGAGAAG
    ACCCCTGATC TGAAAAGGGC TTTAAGGACC CTGCTTAGTA AGATGAAGGA CTCTGATTCG
    CCGCGGCACT CCACTGCCTC CAACAGCTCC ACCTTCAGCA GCCCGCCGAG CCCCGCCTCC
    CCACACAAGA CAAAGTCCCT CTCATTGGAG AGCACAGACC GCACCGCCTG GGACACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150651
    Clone ID Related Accession (Same CDS sequence) XM_021468483.1
    Accession Version XM_021468483.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5388bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase MRCK alpha isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGTCGTCT
    CAACCTAACG ATGAGGAGCG CTTCCAGTTC CCACTTCAGG TGACTGATGT GTCCGAGGAT
    GCTAAAGATC TTGTTCGGCG GCTGATCTGC AGCAGAGAAC ACCGGCTGGG CCAGAACGGT
    ATCGAAGACT TTAAGCAGCA CCCCTTTTTC ACAGGCATCG ACTGGGACAA CATCCGCACA
    TGCGAAGCCC CCTACATCCC TGAAGTCAGC AGTCCCACTG ACACCTCCAA CTTTGATGTG
    GATGACGACT GCCTCAAAAA CTGTGAGACG TTGCCTCCAC CTTCACACAC AGCATTTTCT
    GGACACCACC TCCCATTCGT AGGCTTCACC TACACGAGTA AATGCACGCT GTCAGATCGA
    GGGTGTTTGC GGGAATCGGT CGGACCTGCA CAGGTTGATG CTCATGTTCA GCGTAGCCTG
    GAGGAGAGCC TGGCCTCTGA AGCCTATGAG AGGAGAATTC GACGTTTAGA GCAGGAGAAA
    ACCGAACTTA CCCGTAAACT ACAGGAGTCT ACGCAAACGG TGCAAGCACT GCAGTACCCT
    GATTCAGACG CCCCAGTGAA TGCTAATAAA GAGGTAGAGA TAAGGAGTTT AAAGAGTGAG
    ATCGACATCC TGAAGAAACA GATAGCAGAC TCTGGTCAGA TGGAGCAGCA GTTGGAGAAC
    GCCAGCACAG CTAGAAGAGA CCTGGAAGAC TCGTCCAAGG TCGTCAGGAA CTTAGAGAAA
    CAGCTGAAGA GTGTCACACA AGAGAAAGAT GATCTGCACA AGGAGCTGCT GGGTTTGGCA
    GAGAAGGTGA AGGCTCAGGG GAAGGAGCTA AAGGATGCTC ACAGTCAAAA AAAGATGGCC
    ATAAAGGAGT TCGCTGAGCT GAATGAACAG CTGAGCGATG TTCGCACACA AAAGCAGCGC
    CTCAGCCGAC AGTTCAAAGA CAAGGAGGAG GAGATGGAAG GAGTGTCGCA GAAACTAGAG
    GCTCTACGGC TAGAGATACG CAAAGCCGAG AGAATGCGCA AAGAGTTGGA GGCCCAGGCT
    GAGGAACATG CAGCAGAAGC TCAGAAGGAG AGAAAGGTTC GAGAAAGAAC AGAACAGTAC
    AGCAAACAAC TAGAGGAGGA GCTCGAGGGC ATGAAGCAGT TGAAGCAGAC TGGGCCTCCT
    AACGGAGCTG CAAGCTCAGA CCAGCAGCAG GAGCTGATGC GCGTTCGGGC TGAACTGGAG
    AAGAAGAGCG TACAGTACGA GGAGGAGATG TGTCGCAGAG AGACGCTCCA CAGCAGTGAG
    ATGAAGAACC TCCAAAAAGA GCTACGGGAT GCCGAGGGTC AACACCTCAC ACATCAGAAA
    GAGATACTCG TGCTTAAAGA CAAACTGGAG AAAACTCGCA GAGAAAGTCA AGTCGAGCGA
    GAAGAATTTG TGTGCGAGTA CAAGCAGAAG TACGAGAGGG AAAAAGCCCA TCTGACAGAC
    GAAAACAAGA AGCTGAGTGC CGAAGTGGAG AAGTTGAACG GTCAGTTCGA GCAGCTGAGC
    AGCAGTCACC GGCAGCTGGA GGAGGAGATG AGAGAACTGG CTGATAAGAA GGAGAGTGTT
    GCCCACTGGG AGGCCCAGAT CACCGAGATC ATCCAGTGGG TGAGCGATGA GAAGGATGCC
    CGTGGTTACC TTCAAGCTCT GGCCACCAAA ATGACAGAGG AGCTGGAAGG GCTCAGAAAC
    ACCAGTTTGG GAGCCAGAGG AACGGACATG CCGTGGAAGA TGAGGCGTTT CGCCAAGCTG
    GACATGTCTG CGCGTCTGGA GCTGCAGTCG GCGCTGGATA CTGAGATCAG AGCCAAACAG
    TCCATCCAAG ACGAGCTTAA CAAAGTCAAA GCAACCAACA TTGCCACAGA ATGCAAATTG
    CAAGAATCCG AAAGTAGAAA CCAGGAGCTG CTGGGAGAAA TTGAGAGACT GAGGAAGGAG
    ACAGAGGAGC TAAGGCTGAG GAGAGGCGTG AAGCACCAGG ATTCCCAAAA TTCCTTTTTG
    GCTTTCCTCA ATGCGCCCAC TTCTGCACTG GATCAGTTTG ATCGCTCACC CTCTTTTGGT
    CCCGGAAGCA AAACCAGACG TCTTGACTCT GCAGATTTCA CCCCTTCCAA CACTCCATCC
    AGAGATGAAG ACGCAAGATC TCGCCGTATT TCACGCTCTC GCTCACCCTC CACCGCCAGT
    GACATGGAGC CTATAGAGTT GATTGACCAC ACATCACGTA GTCTTCAGAC CCCAACAGGC
    AGATCAGGCT ACGGGAGCTT GGGACACTCA TCACCTAAGC CCAAGGCACA CCAGTTTAGT
    GTAAAGTCCT TCAATGTTCC CACTAAATGT AACCAGTGCA CCTCTCTCAT GGTGGGACTC
    ATCCGTCAGG GCTGCACATG CGAAGCCTGC AGCTTCTCCT GTCATGTCAC TTGTGCTGAT
    AAAGCCCCTT CTGTGTGCCC CGTCGCACCA GACCAGACCA GGGGCAAACT GGGCATTGAC
    ACTCAGAGGG GCATTGGGAC TGCATATGAG GGGCATCTTA GGGTGCCGAA GCCCACTGGG
    GTGAAGAAGG GTTGGCAGAG GGTCTGGGCA GTGGTTTGTG ACTTCAAACT TTTTCTGTAT
    GAGCTTGGAG AAGGGAAAGG TGCTCAGCCT GGTGTTGTAG TAAACCAGGT CATTGACATG
    AGGGATGAAG AGTTTTCCGC CAGTTCGGTT CTGGAGTCTG ATGTCATCCA TGCTAATAGG
    AAGGATATAC CCTGTATTTT TAGGGTAACA GCATCTCAGC TGTCCGCCTC CTCCAGTCAG
    AAGACGTGTA TTCTAATTCT GGCTGACAAC GAGCAAGAAA GGACGAAGTG GGTGTGCGCT
    CTGAATGAAC TGCATCGCAT CCTGAGGAAA AACAAGCTGA AGGAGCGTTT CGTCTATGTG
    CCTAAAGAAG CCTACGACAG CACACTTCCT CTCATCAAAA CCACCCAAAC TGCTGCTATT
    ATAGATCATG AACGCGTTGC TTTGGGCAAT GAGGAGGGCC TGTTTGTCAT CCACGTCACC
    AAAGATGAAA TCATTCGTGT GGGTGATGTC AAGAAGGTGC ATCATGTAGA ACTGATGCCC
    ACGGAGCACA TACTGGCCGT CATCTCGGGC AGGAACCGAC AAGTATGTCT GGTCTCCATG
    GCGGGTCTCG ACGGGAGGGA GGTCGACAAG AATAAGGTGG CAGATACTAA AAACTGCCAG
    TTGCTGGTGT CCGGTGTGGT TCGCAATGTC ACCTGCTTCT GCCTCGCCAT TAAACGGCAG
    ATCTCATGCT TCGAAATTAA CAAGAGCAAG TCACGACACT CCCATCTCGT GGACATACAA
    GCTCCAGGAA CTGTCCAATG GATGGCGCTG TTCAACCAGC AGTTGTGTGT TGGTTACCCA
    TCAGGCTTCA TGCGCTACAG TTTGTATGGA GAAACACCTC CAGCCAGCTT ACTTCATCCA
    GATGACCCCA CACTGGCATT TATTAAAACA GACAACCTGG ATGCCCTTTG TGCTGTGGAA
    ATCTCCAATA AGGAACTGCT GCTCTGCTTC AGTAAAATAG CAGTGTATGT TGACACACAC
    GGCAGACGCT CGCGCCAGCA GGAGCTGATG TGGCCCGCGG CGCCCACCGC CTGCTGCTAT
    AACGCCCCCT ATCTGTCGGT CTACAGTGAA AATGCAGTGG ATGTGTTTGA TGTGAACTCT
    ACGGAATGGA TTCAGACCAT TCCTCTCAAA AAGGTCCGGC CATTAAACAT GGATGGCTCT
    TTGAATCTCC TGGGCCTAGA AACGGTCAGA CTCATCTACT TCAGAAACAA GACCGCAGAG
    GGGGATGAAC TGGTGGTGCC TGAAGTGTGT GATAACAGCA GAAAGCAGAT GGTGCGAAGC
    ATGAGCAACA AGAGACGATT CTCGTTTCGT ATTCCTGAGG AGGAGAGACT GCAGCAGAGG
    AGAGAAATGT TGAAAGATCC TGAGAGGAGG AACAAGCTGA TCTCAAATCC TACAAATTTT
    AACCATGTGG CTCATATGGG ACCTGGAGAT GGCATGCAGA TACTCAGAGA CCTGCCAATG
    GACAACCGTG CTGGATTCAG CGGCTCCGTC AGCATCCCCT CCATTACAAA AAACAGAGCA
    GAACCAGGGC GATCCATGAG CGCAAGCAGT GGCCTAGGAA TGCGGTCGTC CTCTCAGAAC
    GGCAGTGCTC TAAGGAGGGA GCTGTCCAGC GGCAGCTACA GCTCTAAACG GCAGACCATG
    ACTTCACCCT CCGAGAGTTC GCTGTCATCA GGAGGAGGGA TGGACGTGGG GGAAGCGCCG
    CTCTCTCAGT TTGACAGAGA GTGGCAGGCA GTATCGCCCT CGGAGAAGAC CCCTGATCTG
    AAAAGGGCTT TAAGGACCCT GCTTAGTAAG ATGAAGGACT CTGATTCGCC GCGGCACTCC
    ACTGCCTCCA ACAGCTCCAC CTTCAGCAGC CCGCCGAGCC CCGCCTCCCC ACACAAGACA
    AAGTCCCTCT CATTGGAGAG CACAGACCGC ACCGCCTGGG ACACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324158.1
    CDS1641..7028
    Translation

    Target ORF information:

    RefSeq Version XM_021468483.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468483.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGTCGTCT
    CAACCTAACG ATGAGGAGCG CTTCCAGTTC CCACTTCAGG TGACTGATGT GTCCGAGGAT
    GCTAAAGATC TTGTTCGGCG GCTGATCTGC AGCAGAGAAC ACCGGCTGGG CCAGAACGGT
    ATCGAAGACT TTAAGCAGCA CCCCTTTTTC ACAGGCATCG ACTGGGACAA CATCCGCACA
    TGCGAAGCCC CCTACATCCC TGAAGTCAGC AGTCCCACTG ACACCTCCAA CTTTGATGTG
    GATGACGACT GCCTCAAAAA CTGTGAGACG TTGCCTCCAC CTTCACACAC AGCATTTTCT
    GGACACCACC TCCCATTCGT AGGCTTCACC TACACGAGTA AATGCACGCT GTCAGATCGA
    GGGTGTTTGC GGGAATCGGT CGGACCTGCA CAGGTTGATG CTCATGTTCA GCGTAGCCTG
    GAGGAGAGCC TGGCCTCTGA AGCCTATGAG AGGAGAATTC GACGTTTAGA GCAGGAGAAA
    ACCGAACTTA CCCGTAAACT ACAGGAGTCT ACGCAAACGG TGCAAGCACT GCAGTACCCT
    GATTCAGACG CCCCAGTGAA TGCTAATAAA GAGGTAGAGA TAAGGAGTTT AAAGAGTGAG
    ATCGACATCC TGAAGAAACA GATAGCAGAC TCTGGTCAGA TGGAGCAGCA GTTGGAGAAC
    GCCAGCACAG CTAGAAGAGA CCTGGAAGAC TCGTCCAAGG TCGTCAGGAA CTTAGAGAAA
    CAGCTGAAGA GTGTCACACA AGAGAAAGAT GATCTGCACA AGGAGCTGCT GGGTTTGGCA
    GAGAAGGTGA AGGCTCAGGG GAAGGAGCTA AAGGATGCTC ACAGTCAAAA AAAGATGGCC
    ATAAAGGAGT TCGCTGAGCT GAATGAACAG CTGAGCGATG TTCGCACACA AAAGCAGCGC
    CTCAGCCGAC AGTTCAAAGA CAAGGAGGAG GAGATGGAAG GAGTGTCGCA GAAACTAGAG
    GCTCTACGGC TAGAGATACG CAAAGCCGAG AGAATGCGCA AAGAGTTGGA GGCCCAGGCT
    GAGGAACATG CAGCAGAAGC TCAGAAGGAG AGAAAGGTTC GAGAAAGAAC AGAACAGTAC
    AGCAAACAAC TAGAGGAGGA GCTCGAGGGC ATGAAGCAGT TGAAGCAGAC TGGGCCTCCT
    AACGGAGCTG CAAGCTCAGA CCAGCAGCAG GAGCTGATGC GCGTTCGGGC TGAACTGGAG
    AAGAAGAGCG TACAGTACGA GGAGGAGATG TGTCGCAGAG AGACGCTCCA CAGCAGTGAG
    ATGAAGAACC TCCAAAAAGA GCTACGGGAT GCCGAGGGTC AACACCTCAC ACATCAGAAA
    GAGATACTCG TGCTTAAAGA CAAACTGGAG AAAACTCGCA GAGAAAGTCA AGTCGAGCGA
    GAAGAATTTG TGTGCGAGTA CAAGCAGAAG TACGAGAGGG AAAAAGCCCA TCTGACAGAC
    GAAAACAAGA AGCTGAGTGC CGAAGTGGAG AAGTTGAACG GTCAGTTCGA GCAGCTGAGC
    AGCAGTCACC GGCAGCTGGA GGAGGAGATG AGAGAACTGG CTGATAAGAA GGAGAGTGTT
    GCCCACTGGG AGGCCCAGAT CACCGAGATC ATCCAGTGGG TGAGCGATGA GAAGGATGCC
    CGTGGTTACC TTCAAGCTCT GGCCACCAAA ATGACAGAGG AGCTGGAAGG GCTCAGAAAC
    ACCAGTTTGG GAGCCAGAGG AACGGACATG CCGTGGAAGA TGAGGCGTTT CGCCAAGCTG
    GACATGTCTG CGCGTCTGGA GCTGCAGTCG GCGCTGGATA CTGAGATCAG AGCCAAACAG
    TCCATCCAAG ACGAGCTTAA CAAAGTCAAA GCAACCAACA TTGCCACAGA ATGCAAATTG
    CAAGAATCCG AAAGTAGAAA CCAGGAGCTG CTGGGAGAAA TTGAGAGACT GAGGAAGGAG
    ACAGAGGAGC TAAGGCTGAG GAGAGGCGTG AAGCACCAGG ATTCCCAAAA TTCCTTTTTG
    GCTTTCCTCA ATGCGCCCAC TTCTGCACTG GATCAGTTTG ATCGCTCACC CTCTTTTGGT
    CCCGGAAGCA AAACCAGACG TCTTGACTCT GCAGATTTCA CCCCTTCCAA CACTCCATCC
    AGAGATGAAG ACGCAAGATC TCGCCGTATT TCACGCTCTC GCTCACCCTC CACCGCCAGT
    GACATGGAGC CTATAGAGTT GATTGACCAC ACATCACGTA GTCTTCAGAC CCCAACAGGC
    AGATCAGGCT ACGGGAGCTT GGGACACTCA TCACCTAAGC CCAAGGCACA CCAGTTTAGT
    GTAAAGTCCT TCAATGTTCC CACTAAATGT AACCAGTGCA CCTCTCTCAT GGTGGGACTC
    ATCCGTCAGG GCTGCACATG CGAAGCCTGC AGCTTCTCCT GTCATGTCAC TTGTGCTGAT
    AAAGCCCCTT CTGTGTGCCC CGTCGCACCA GACCAGACCA GGGGCAAACT GGGCATTGAC
    ACTCAGAGGG GCATTGGGAC TGCATATGAG GGGCATCTTA GGGTGCCGAA GCCCACTGGG
    GTGAAGAAGG GTTGGCAGAG GGTCTGGGCA GTGGTTTGTG ACTTCAAACT TTTTCTGTAT
    GAGCTTGGAG AAGGGAAAGG TGCTCAGCCT GGTGTTGTAG TAAACCAGGT CATTGACATG
    AGGGATGAAG AGTTTTCCGC CAGTTCGGTT CTGGAGTCTG ATGTCATCCA TGCTAATAGG
    AAGGATATAC CCTGTATTTT TAGGGTAACA GCATCTCAGC TGTCCGCCTC CTCCAGTCAG
    AAGACGTGTA TTCTAATTCT GGCTGACAAC GAGCAAGAAA GGACGAAGTG GGTGTGCGCT
    CTGAATGAAC TGCATCGCAT CCTGAGGAAA AACAAGCTGA AGGAGCGTTT CGTCTATGTG
    CCTAAAGAAG CCTACGACAG CACACTTCCT CTCATCAAAA CCACCCAAAC TGCTGCTATT
    ATAGATCATG AACGCGTTGC TTTGGGCAAT GAGGAGGGCC TGTTTGTCAT CCACGTCACC
    AAAGATGAAA TCATTCGTGT GGGTGATGTC AAGAAGGTGC ATCATGTAGA ACTGATGCCC
    ACGGAGCACA TACTGGCCGT CATCTCGGGC AGGAACCGAC AAGTATGTCT GGTCTCCATG
    GCGGGTCTCG ACGGGAGGGA GGTCGACAAG AATAAGGTGG CAGATACTAA AAACTGCCAG
    TTGCTGGTGT CCGGTGTGGT TCGCAATGTC ACCTGCTTCT GCCTCGCCAT TAAACGGCAG
    ATCTCATGCT TCGAAATTAA CAAGAGCAAG TCACGACACT CCCATCTCGT GGACATACAA
    GCTCCAGGAA CTGTCCAATG GATGGCGCTG TTCAACCAGC AGTTGTGTGT TGGTTACCCA
    TCAGGCTTCA TGCGCTACAG TTTGTATGGA GAAACACCTC CAGCCAGCTT ACTTCATCCA
    GATGACCCCA CACTGGCATT TATTAAAACA GACAACCTGG ATGCCCTTTG TGCTGTGGAA
    ATCTCCAATA AGGAACTGCT GCTCTGCTTC AGTAAAATAG CAGTGTATGT TGACACACAC
    GGCAGACGCT CGCGCCAGCA GGAGCTGATG TGGCCCGCGG CGCCCACCGC CTGCTGCTAT
    AACGCCCCCT ATCTGTCGGT CTACAGTGAA AATGCAGTGG ATGTGTTTGA TGTGAACTCT
    ACGGAATGGA TTCAGACCAT TCCTCTCAAA AAGGTCCGGC CATTAAACAT GGATGGCTCT
    TTGAATCTCC TGGGCCTAGA AACGGTCAGA CTCATCTACT TCAGAAACAA GACCGCAGAG
    GGGGATGAAC TGGTGGTGCC TGAAGTGTGT GATAACAGCA GAAAGCAGAT GGTGCGAAGC
    ATGAGCAACA AGAGACGATT CTCGTTTCGT ATTCCTGAGG AGGAGAGACT GCAGCAGAGG
    AGAGAAATGT TGAAAGATCC TGAGAGGAGG AACAAGCTGA TCTCAAATCC TACAAATTTT
    AACCATGTGG CTCATATGGG ACCTGGAGAT GGCATGCAGA TACTCAGAGA CCTGCCAATG
    GACAACCGTG CTGGATTCAG CGGCTCCGTC AGCATCCCCT CCATTACAAA AAACAGAGCA
    GAACCAGGGC GATCCATGAG CGCAAGCAGT GGCCTAGGAA TGCGGTCGTC CTCTCAGAAC
    GGCAGTGCTC TAAGGAGGGA GCTGTCCAGC GGCAGCTACA GCTCTAAACG GCAGACCATG
    ACTTCACCCT CCGAGAGTTC GCTGTCATCA GGAGGAGGGA TGGACGTGGG GGAAGCGCCG
    CTCTCTCAGT TTGACAGAGA GTGGCAGGCA GTATCGCCCT CGGAGAAGAC CCCTGATCTG
    AAAAGGGCTT TAAGGACCCT GCTTAGTAAG ATGAAGGACT CTGATTCGCC GCGGCACTCC
    ACTGCCTCCA ACAGCTCCAC CTTCAGCAGC CCGCCGAGCC CCGCCTCCCC ACACAAGACA
    AAGTCCCTCT CATTGGAGAG CACAGACCGC ACCGCCTGGG ACACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150652
    Clone ID Related Accession (Same CDS sequence) XM_021468485.1
    Accession Version XM_021468485.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5364bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase MRCK alpha isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGTCGTCT
    CAACCTAACG ATGAGGAGCG CTTCCAGTTC CCACTTCAGG TGACTGATGT GTCCGAGGAT
    GCTAAAGATC TTGTTCGGCG GCTGATCTGC AGCAGAGAAC ACCGGCTGGG CCAGAACGGT
    ATCGAAGACT TTAAGCAGCA CCCCTTTTTC ACAGGCATCG ACTGGGACAA CATCCGCACA
    TGCGAAGCCC CCTACATCCC TGAAGTCAGC AGTCCCACTG ACACCTCCAA CTTTGATGTG
    GATGACGACT GCCTCAAAAA CTGTGAGACG TTGCCTCCAC CTTCACACAC AGCATTTTCT
    GGACACCACC TCCCATTCGT AGGCTTCACC TACACGAGTA AATGCACGCT GTCAGATCGA
    GGGTGTTTGC GGGAATCGGT CGGACCTGCA CAGGTTGATG CTCATGTTCA GCGTAGCCTG
    GAGGAGAGCC TGGCCTCTGA AGCCTATGAG AGGAGAATTC GACGTTTAGA GCAGGAGAAA
    ACCGAACTTA CCCGTAAACT ACAGGAGTCT ACGCAAACGG TGCAAGCACT GCAGTACCCT
    GATTCAGACG CCCCAGTGAA TGCTAATAAA GAGGTAGAGA TAAGGAGTTT AAAGAGTGAG
    ATCGACATCC TGAAGAAACA GATAGCAGAC TCTGGTCAGA TGGAGCAGCA GTTGGAGAAC
    GCCAGCACAG CTAGAAGAGA CCTGGAAGAC TCGTCCAAGG TCGTCAGGAA CTTAGAGAAA
    CAGCTGAAGA GTGTCACACA AGAGAAAGAT GATCTGCACA AGGAGCTGCT GGGTTTGGCA
    GAGAAGGTGA AGGCTCAGGG GAAGGAGCTA AAGGATGCTC ACAGTCAAAA AAAGATGGCC
    ATAAAGGAGT TCGCTGAGCT GAATGAACAG CTGAGCGATG TTCGCACACA AAAGCAGCGC
    CTCAGCCGAC AGTTCAAAGA CAAGGAGGAG GAGATGGAAG GAGTGTCGCA GAAACTAGAG
    GCTCTACGGC TAGAGATACG CAAAGCCGAG AGAATGCGCA AAGAGTTGGA GGCCCAGGCT
    GAGGAACATG CAGCAGAAGC TCAGAAGGAG AGAAAGGTTC GAGAAAGAAC AGAACAGTAC
    AGCAAACAAC TAGAGGAGGA GCTCGAGGGC ATGAAGCAGT TGAAGCAGAC TGGGCCTCCT
    AACGGAGCTG CAAGCTCAGA CCAGCAGCAG GAGCTGATGC GCGTTCGGGC TGAACTGGAG
    AAGAAGAGCG TACAGTACGA GGAGGAGATG TGTCGCAGAG AGACGCTCCA CAGCAGTGAG
    ATGAAGAACC TCCAAAAAGA GCTACGGGAT GCCGAGGGTC AACACCTCAC ACATCAGAAA
    GAGATACTCG TGCTTAAAGA CAAACTGGAG AAAACTCGCA GAGAAAGTCA AGTCGAGCGA
    GAAGAATTTG TGTGCGAGTA CAAGCAGAAG TACGAGAGGG AAAAAGCCCA TCTGACAGAC
    GAAAACAAGA AGCTGAGTGC CGAAGTGGAG AAGTTGAACG GTCAGTTCGA GCAGCTGAGC
    AGCAGTCACC GGCAGCTGGA GGAGGAGATG AGAGAACTGG CTGATAAGAA GGAGAGTGTT
    GCCCACTGGG AGGCCCAGAT CACCGAGATC ATCCAGTGGG TGAGCGATGA GAAGGATGCC
    CGTGGTTACC TTCAAGCTCT GGCCACCAAA ATGACAGAGG AGCTGGAAGG GCTCAGAAAC
    ACCAGTTTGG GAGCCAGAGG AACGGACATG CCGTGGAAGA TGAGGCGTTT CGCCAAGCTG
    GACATGTCTG CGCGTCTGGA GCTGCAGTCG GCGCTGGATA CTGAGATCAG AGCCAAACAG
    TCCATCCAAG ACGAGCTTAA CAAAGTCAAA GCAACCAACA TTGCCACAGA ATGCAAATTG
    CAAGAATCCG AAAGTAGAAA CCAGGAGCTG CTGGGAGAAA TTGAGAGACT GAGGAAGGAG
    ACAGAGGAGC TAAGGCTGAG GAGAGGCGTG AAGCACCAGG ATTCCCAAAA TTCCTTTTTG
    GCTTTCCTCA ATGCGCCCAC TTCTGCACTG GATCAGTTTG ATCTTGACTC TGCAGATTTC
    ACCCCTTCCA ACACTCCATC CAGAGATGAA GACGCAAGAT CTCGCCGTAT TTCACGCTCT
    CGCTCACCCT CCACCGCCAG TGACATGGAG CCTATAGAGT TGATTGACCA CACATCACGT
    AGTCTTCAGA CCCCAACAGG CAGATCAGGC TACGGGAGCT TGGGACACTC ATCACCTAAG
    CCCAAGGCAC ACCAGTTTAG TGTAAAGTCC TTCAATGTTC CCACTAAATG TAACCAGTGC
    ACCTCTCTCA TGGTGGGACT CATCCGTCAG GGCTGCACAT GCGAAGCCTG CAGCTTCTCC
    TGTCATGTCA CTTGTGCTGA TAAAGCCCCT TCTGTGTGCC CCGTCGCACC AGACCAGACC
    AGGGGCAAAC TGGGCATTGA CACTCAGAGG GGCATTGGGA CTGCATATGA GGGGCATCTT
    AGGGTGCCGA AGCCCACTGG GGTGAAGAAG GGTTGGCAGA GGGTCTGGGC AGTGGTTTGT
    GACTTCAAAC TTTTTCTGTA TGAGCTTGGA GAAGGGAAAG GTGCTCAGCC TGGTGTTGTA
    GTAAACCAGG TCATTGACAT GAGGGATGAA GAGTTTTCCG CCAGTTCGGT TCTGGAGTCT
    GATGTCATCC ATGCTAATAG GAAGGATATA CCCTGTATTT TTAGGGTAAC AGCATCTCAG
    CTGTCCGCCT CCTCCAGTCA GAAGACGTGT ATTCTAATTC TGGCTGACAA CGAGCAAGAA
    AGGACGAAGT GGGTGTGCGC TCTGAATGAA CTGCATCGCA TCCTGAGGAA AAACAAGCTG
    AAGGAGCGTT TCGTCTATGT GCCTAAAGAA GCCTACGACA GCACACTTCC TCTCATCAAA
    ACCACCCAAA CTGCTGCTAT TATAGATCAT GAACGCGTTG CTTTGGGCAA TGAGGAGGGC
    CTGTTTGTCA TCCACGTCAC CAAAGATGAA ATCATTCGTG TGGGTGATGT CAAGAAGGTG
    CATCATGTAG AACTGATGCC CACGGAGCAC ATACTGGCCG TCATCTCGGG CAGGAACCGA
    CAAGTATGTC TGGTCTCCAT GGCGGGTCTC GACGGGAGGG AGGTCGACAA GAATAAGGTG
    GCAGATACTA AAAACTGCCA GTTGCTGGTG TCCGGTGTGG TTCGCAATGT CACCTGCTTC
    TGCCTCGCCA TTAAACGGCA GATCTCATGC TTCGAAATTA ACAAGAGCAA GTCACGACAC
    TCCCATCTCG TGGACATACA AGCTCCAGGA ACTGTCCAAT GGATGGCGCT GTTCAACCAG
    CAGTTGTGTG TTGGTTACCC ATCAGGCTTC ATGCGCTACA GTTTGTATGG AGAAACACCT
    CCAGCCAGCT TACTTCATCC AGATGACCCC ACACTGGCAT TTATTAAAAC AGACAACCTG
    GATGCCCTTT GTGCTGTGGA AATCTCCAAT AAGGAACTGC TGCTCTGCTT CAGTAAAATA
    GCAGTGTATG TTGACACACA CGGCAGACGC TCGCGCCAGC AGGAGCTGAT GTGGCCCGCG
    GCGCCCACCG CCTGCTGCTA TAACGCCCCC TATCTGTCGG TCTACAGTGA AAATGCAGTG
    GATGTGTTTG ATGTGAACTC TACGGAATGG ATTCAGACCA TTCCTCTCAA AAAGGTCCGG
    CCATTAAACA TGGATGGCTC TTTGAATCTC CTGGGCCTAG AAACGGTCAG ACTCATCTAC
    TTCAGAAACA AGACCGCAGA GGGGGATGAA CTGGTGGTGC CTGAAGTGTG TGATAACAGC
    AGAAAGCAGA TGGTGCGAAG CATGAGCAAC AAGAGACGAT TCTCGTTTCG TATTCCTGAG
    GAGGAGAGAC TGCAGCAGAG GAGAGAAATG TTGAAAGATC CTGAGAGGAG GAACAAGCTG
    ATCTCAAATC CTACAAATTT TAACCATGTG GCTCATATGG GACCTGGAGA TGGCATGCAG
    ATACTCAGAG ACCTGCCAAT GAATGTACGT GCTCAGGACA ACCGTGCTGG ATTCAGCGGC
    TCCGTCAGCA TCCCCTCCAT TACAAAAAAC AGAGCAGAAC CAGGGCGATC CATGAGCGCA
    AGCAGTGGCC TAGGAATGCG GTCGTCCTCT CAGAACGGCA GTGCTCTAAG GAGGGAGCTG
    TCCAGCGGCA GCTACAGCTC TAAACGGCAG ACCATGACTT CACCCTCCGA GAGTTCGCTG
    TCATCAGGAG GAGGGATGGA CGTGGGGGAA GCGCCGCTCT CTCAGTTTGA CAGAGAGTGG
    CAGGCAGTAT CGCCCTCGGA GAAGACCCCT GATCTGAAAA GGGCTTTAAG GACCCTGCTT
    AGTAAGATGA AGGACTCTGA TTCGCCGCGG CACTCCACTG CCTCCAACAG CTCCACCTTC
    AGCAGCCCGC CGAGCCCCGC CTCCCCACAC AAGACAAAGT CCCTCTCATT GGAGAGCACA
    GACCGCACCG CCTGGGACAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324160.1
    CDS1641..7004
    Translation

    Target ORF information:

    RefSeq Version XM_021468485.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468485.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGTCGTCT
    CAACCTAACG ATGAGGAGCG CTTCCAGTTC CCACTTCAGG TGACTGATGT GTCCGAGGAT
    GCTAAAGATC TTGTTCGGCG GCTGATCTGC AGCAGAGAAC ACCGGCTGGG CCAGAACGGT
    ATCGAAGACT TTAAGCAGCA CCCCTTTTTC ACAGGCATCG ACTGGGACAA CATCCGCACA
    TGCGAAGCCC CCTACATCCC TGAAGTCAGC AGTCCCACTG ACACCTCCAA CTTTGATGTG
    GATGACGACT GCCTCAAAAA CTGTGAGACG TTGCCTCCAC CTTCACACAC AGCATTTTCT
    GGACACCACC TCCCATTCGT AGGCTTCACC TACACGAGTA AATGCACGCT GTCAGATCGA
    GGGTGTTTGC GGGAATCGGT CGGACCTGCA CAGGTTGATG CTCATGTTCA GCGTAGCCTG
    GAGGAGAGCC TGGCCTCTGA AGCCTATGAG AGGAGAATTC GACGTTTAGA GCAGGAGAAA
    ACCGAACTTA CCCGTAAACT ACAGGAGTCT ACGCAAACGG TGCAAGCACT GCAGTACCCT
    GATTCAGACG CCCCAGTGAA TGCTAATAAA GAGGTAGAGA TAAGGAGTTT AAAGAGTGAG
    ATCGACATCC TGAAGAAACA GATAGCAGAC TCTGGTCAGA TGGAGCAGCA GTTGGAGAAC
    GCCAGCACAG CTAGAAGAGA CCTGGAAGAC TCGTCCAAGG TCGTCAGGAA CTTAGAGAAA
    CAGCTGAAGA GTGTCACACA AGAGAAAGAT GATCTGCACA AGGAGCTGCT GGGTTTGGCA
    GAGAAGGTGA AGGCTCAGGG GAAGGAGCTA AAGGATGCTC ACAGTCAAAA AAAGATGGCC
    ATAAAGGAGT TCGCTGAGCT GAATGAACAG CTGAGCGATG TTCGCACACA AAAGCAGCGC
    CTCAGCCGAC AGTTCAAAGA CAAGGAGGAG GAGATGGAAG GAGTGTCGCA GAAACTAGAG
    GCTCTACGGC TAGAGATACG CAAAGCCGAG AGAATGCGCA AAGAGTTGGA GGCCCAGGCT
    GAGGAACATG CAGCAGAAGC TCAGAAGGAG AGAAAGGTTC GAGAAAGAAC AGAACAGTAC
    AGCAAACAAC TAGAGGAGGA GCTCGAGGGC ATGAAGCAGT TGAAGCAGAC TGGGCCTCCT
    AACGGAGCTG CAAGCTCAGA CCAGCAGCAG GAGCTGATGC GCGTTCGGGC TGAACTGGAG
    AAGAAGAGCG TACAGTACGA GGAGGAGATG TGTCGCAGAG AGACGCTCCA CAGCAGTGAG
    ATGAAGAACC TCCAAAAAGA GCTACGGGAT GCCGAGGGTC AACACCTCAC ACATCAGAAA
    GAGATACTCG TGCTTAAAGA CAAACTGGAG AAAACTCGCA GAGAAAGTCA AGTCGAGCGA
    GAAGAATTTG TGTGCGAGTA CAAGCAGAAG TACGAGAGGG AAAAAGCCCA TCTGACAGAC
    GAAAACAAGA AGCTGAGTGC CGAAGTGGAG AAGTTGAACG GTCAGTTCGA GCAGCTGAGC
    AGCAGTCACC GGCAGCTGGA GGAGGAGATG AGAGAACTGG CTGATAAGAA GGAGAGTGTT
    GCCCACTGGG AGGCCCAGAT CACCGAGATC ATCCAGTGGG TGAGCGATGA GAAGGATGCC
    CGTGGTTACC TTCAAGCTCT GGCCACCAAA ATGACAGAGG AGCTGGAAGG GCTCAGAAAC
    ACCAGTTTGG GAGCCAGAGG AACGGACATG CCGTGGAAGA TGAGGCGTTT CGCCAAGCTG
    GACATGTCTG CGCGTCTGGA GCTGCAGTCG GCGCTGGATA CTGAGATCAG AGCCAAACAG
    TCCATCCAAG ACGAGCTTAA CAAAGTCAAA GCAACCAACA TTGCCACAGA ATGCAAATTG
    CAAGAATCCG AAAGTAGAAA CCAGGAGCTG CTGGGAGAAA TTGAGAGACT GAGGAAGGAG
    ACAGAGGAGC TAAGGCTGAG GAGAGGCGTG AAGCACCAGG ATTCCCAAAA TTCCTTTTTG
    GCTTTCCTCA ATGCGCCCAC TTCTGCACTG GATCAGTTTG ATCTTGACTC TGCAGATTTC
    ACCCCTTCCA ACACTCCATC CAGAGATGAA GACGCAAGAT CTCGCCGTAT TTCACGCTCT
    CGCTCACCCT CCACCGCCAG TGACATGGAG CCTATAGAGT TGATTGACCA CACATCACGT
    AGTCTTCAGA CCCCAACAGG CAGATCAGGC TACGGGAGCT TGGGACACTC ATCACCTAAG
    CCCAAGGCAC ACCAGTTTAG TGTAAAGTCC TTCAATGTTC CCACTAAATG TAACCAGTGC
    ACCTCTCTCA TGGTGGGACT CATCCGTCAG GGCTGCACAT GCGAAGCCTG CAGCTTCTCC
    TGTCATGTCA CTTGTGCTGA TAAAGCCCCT TCTGTGTGCC CCGTCGCACC AGACCAGACC
    AGGGGCAAAC TGGGCATTGA CACTCAGAGG GGCATTGGGA CTGCATATGA GGGGCATCTT
    AGGGTGCCGA AGCCCACTGG GGTGAAGAAG GGTTGGCAGA GGGTCTGGGC AGTGGTTTGT
    GACTTCAAAC TTTTTCTGTA TGAGCTTGGA GAAGGGAAAG GTGCTCAGCC TGGTGTTGTA
    GTAAACCAGG TCATTGACAT GAGGGATGAA GAGTTTTCCG CCAGTTCGGT TCTGGAGTCT
    GATGTCATCC ATGCTAATAG GAAGGATATA CCCTGTATTT TTAGGGTAAC AGCATCTCAG
    CTGTCCGCCT CCTCCAGTCA GAAGACGTGT ATTCTAATTC TGGCTGACAA CGAGCAAGAA
    AGGACGAAGT GGGTGTGCGC TCTGAATGAA CTGCATCGCA TCCTGAGGAA AAACAAGCTG
    AAGGAGCGTT TCGTCTATGT GCCTAAAGAA GCCTACGACA GCACACTTCC TCTCATCAAA
    ACCACCCAAA CTGCTGCTAT TATAGATCAT GAACGCGTTG CTTTGGGCAA TGAGGAGGGC
    CTGTTTGTCA TCCACGTCAC CAAAGATGAA ATCATTCGTG TGGGTGATGT CAAGAAGGTG
    CATCATGTAG AACTGATGCC CACGGAGCAC ATACTGGCCG TCATCTCGGG CAGGAACCGA
    CAAGTATGTC TGGTCTCCAT GGCGGGTCTC GACGGGAGGG AGGTCGACAA GAATAAGGTG
    GCAGATACTA AAAACTGCCA GTTGCTGGTG TCCGGTGTGG TTCGCAATGT CACCTGCTTC
    TGCCTCGCCA TTAAACGGCA GATCTCATGC TTCGAAATTA ACAAGAGCAA GTCACGACAC
    TCCCATCTCG TGGACATACA AGCTCCAGGA ACTGTCCAAT GGATGGCGCT GTTCAACCAG
    CAGTTGTGTG TTGGTTACCC ATCAGGCTTC ATGCGCTACA GTTTGTATGG AGAAACACCT
    CCAGCCAGCT TACTTCATCC AGATGACCCC ACACTGGCAT TTATTAAAAC AGACAACCTG
    GATGCCCTTT GTGCTGTGGA AATCTCCAAT AAGGAACTGC TGCTCTGCTT CAGTAAAATA
    GCAGTGTATG TTGACACACA CGGCAGACGC TCGCGCCAGC AGGAGCTGAT GTGGCCCGCG
    GCGCCCACCG CCTGCTGCTA TAACGCCCCC TATCTGTCGG TCTACAGTGA AAATGCAGTG
    GATGTGTTTG ATGTGAACTC TACGGAATGG ATTCAGACCA TTCCTCTCAA AAAGGTCCGG
    CCATTAAACA TGGATGGCTC TTTGAATCTC CTGGGCCTAG AAACGGTCAG ACTCATCTAC
    TTCAGAAACA AGACCGCAGA GGGGGATGAA CTGGTGGTGC CTGAAGTGTG TGATAACAGC
    AGAAAGCAGA TGGTGCGAAG CATGAGCAAC AAGAGACGAT TCTCGTTTCG TATTCCTGAG
    GAGGAGAGAC TGCAGCAGAG GAGAGAAATG TTGAAAGATC CTGAGAGGAG GAACAAGCTG
    ATCTCAAATC CTACAAATTT TAACCATGTG GCTCATATGG GACCTGGAGA TGGCATGCAG
    ATACTCAGAG ACCTGCCAAT GAATGTACGT GCTCAGGACA ACCGTGCTGG ATTCAGCGGC
    TCCGTCAGCA TCCCCTCCAT TACAAAAAAC AGAGCAGAAC CAGGGCGATC CATGAGCGCA
    AGCAGTGGCC TAGGAATGCG GTCGTCCTCT CAGAACGGCA GTGCTCTAAG GAGGGAGCTG
    TCCAGCGGCA GCTACAGCTC TAAACGGCAG ACCATGACTT CACCCTCCGA GAGTTCGCTG
    TCATCAGGAG GAGGGATGGA CGTGGGGGAA GCGCCGCTCT CTCAGTTTGA CAGAGAGTGG
    CAGGCAGTAT CGCCCTCGGA GAAGACCCCT GATCTGAAAA GGGCTTTAAG GACCCTGCTT
    AGTAAGATGA AGGACTCTGA TTCGCCGCGG CACTCCACTG CCTCCAACAG CTCCACCTTC
    AGCAGCCCGC CGAGCCCCGC CTCCCCACAC AAGACAAAGT CCCTCTCATT GGAGAGCACA
    GACCGCACCG CCTGGGACAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150653
    Clone ID Related Accession (Same CDS sequence) XM_021468486.1
    Accession Version XM_021468486.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase MRCK alpha isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGTCGTCT
    CAACCTAACG ATGAGGAGCG CTTCCAGTTC CCACTTCAGG TGACTGATGT GTCCGAGGAT
    GCTAAAGATC TTGTTCGGCG GCTGATCTGC AGCAGAGAAC ACCGGCTGGG CCAGAACGGT
    ATCGAAGACT TTAAGCAGCA CCCCTTTTTC ACAGGCATCG ACTGGGACAA CATCCGCACA
    TGCGAAGCCC CCTACATCCC TGAAGTCAGC AGTCCCACTG ACACCTCCAA CTTTGATGTG
    GATGACGACT GCCTCAAAAA CTGTGAGACG TTGCCTCCAC CTTCACACAC AGCATTTTCT
    GGACACCACC TCCCATTCGT AGGCTTCACC TACACGAGTA AATGCACGCT GTCAGATCGA
    GGGTGTTTGC GGGAATCGGT CGGACCTGCA CAGGTTGATG CTCATGTTCA GCGTAGCCTG
    GAGGAGAGCC TGGCCTCTGA AGCCTATGAG AGGAGAATTC GACGTTTAGA GCAGGAGAAA
    ACCGAACTTA CCCGTAAACT ACAGGAGTCT ACGCAAACGG TGCAAGCACT GCAGTACCCT
    GATTCAGACG CCCCAGTGAA TGCTAATAAA GAGGTAGAGA TAAGGAGTTT AAAGAGTGAG
    ATCGACATCC TGAAGAAACA GATAGCAGAC TCTGGTCAGA TGGAGCAGCA GTTGGAGAAC
    GCCAGCACAG CTAGAAGAGA CCTGGAAGAC TCGTCCAAGG TCGTCAGGAA CTTAGAGAAA
    CAGCTGAAGA GTGTCACACA AGAGAAAGAT GATCTGCACA AGGAGCTGCT GGGTTTGGCA
    GAGAAGGTGA AGGCTCAGGG GAAGGAGCTA AAGGATGCTC ACAGTCAAAA AAAGATGGCC
    ATAAAGGAGT TCGCTGAGCT GAATGAACAG CTGAGCGATG TTCGCACACA AAAGCAGCGC
    CTCAGCCGAC AGTTCAAAGA CAAGGAGGAG GAGATGGAAG GAGTGTCGCA GAAACTAGAG
    GCTCTACGGC TAGAGATACG CAAAGCCGAG AGAATGCGCA AAGAGTTGGA GGCCCAGGCT
    GAGGAACATG CAGCAGAAGC TCAGAAGGAG AGAAAGGTTC GAGAAAGAAC AGAACAGTAC
    AGCAAACAAC TAGAGGAGGA GCTCGAGGGC ATGAAGCAGT TGAAGCAGAC TGGGCCTCCT
    AACGGAGCTG CAAGCTCAGA CCAGCAGCAG GAGCTGATGC GCGTTCGGGC TGAACTGGAG
    AAGAAGAGCG TACAGTACGA GGAGGAGATG TGTCGCAGAG AGACGCTCCA CAGCAGTGAG
    ATGAAGAACC TCCAAAAAGA GCTACGGGAT GCCGAGGGTC AACACCTCAC ACATCAGAAA
    GAGATACTCG TGCTTAAAGA CAAACTGGAG AAAACTCGCA GAGAAAGTCA AGTCGAGCGA
    GAAGAATTTG TGTGCGAGTA CAAGCAGAAG TACGAGAGGG AAAAAGCCCA TCTGACAGAC
    GAAAACAAGA AGCTGAGTGC CGAAGTGGAG AAGTTGAACG GTCAGTTCGA GCAGCTGAGC
    AGCAGTCACC GGCAGCTGGA GGAGGAGATG AGAGAACTGG CTGATAAGAA GGAGAGTGTT
    GCCCACTGGG AGGCCCAGAT CACCGAGATC ATCCAGTGGG TGAGCGATGA GAAGGATGCC
    CGTGGTTACC TTCAAGCTCT GGCCACCAAA ATGACAGAGG AGCTGGAAGG GCTCAGAAAC
    ACCAGTTTGG GAGCCAGAGG AACGGACATG CCGTGGAAGA TGAGGCGTTT CGCCAAGCTG
    GACATGTCTG CGCGTCTGGA GCTGCAGTCG GCGCTGGATA CTGAGATCAG AGCCAAACAG
    TCCATCCAAG ACGAGCTTAA CAAAGTCAAA GCAACCAACA TTGCCACAGA ATGCAAATTG
    CAAGAATCCG AAAGTAGAAA CCAGGAGCTG CTGGGAGAAA TTGAGAGACT GAGGAAGGAG
    ACAGAGGAGC TAAGGCTGAG GAGAGGCGTG AAGCACCAGG ATTCCCAAAA TTCCTTTTTG
    GCTTTCCTCA ATGCGCCCAC TTCTGCACTG GATCAGTTTG ATCGCTCACC CTCTTTTGGT
    CCCGGAAGCA AAACCAGACG TCTTGACTCT GCAGATTTCA CCCCTTCCAA CACTCCATCC
    AGAGATGAAG ACGCAAGATC TCGCCGTATT TCACGCTCTC GCTCACCCTC CACCGCCAGT
    GACATGGAGC CTATAGAGTT GATTGACCAC ACATCACGTA GTCTTCAGAC CCCAACAGGC
    AGATCAGGCT ACGGGAGCTT GGGACACTCA TCACCTAAGC CCAAGGCACA CCAGTTTAGT
    GTAAAGTCCT TCAATGTTCC CACTAAATGT AACCAGTGCA CCTCTCTCAT GGTGGGACTC
    ATCCGTCAGG GCTGCACATG CGAAGCCTGC AGCTTCTCCT GTCATGTCAC TTGTGCTGAT
    AAAGCCCCTT CTGTGTGCCC CGTCGCACCA GACCAGACCA GGGGCAAACT GGGCATTGAC
    ACTCAGAGGG GCATTGGGAC TGCATATGAG GGGCATCTTA GGGTGCCGAA GCCCACTGGG
    GTGAAGAAGG GTTGGCAGAG GGTCTGGGCA GTGGTTTGTG ACTTCAAACT TTTTCTGTAT
    GAGCTTGGAG AAGGGAAAGG TGCTCAGCCT GGTGTTGTAG TAAACCAGGT CATTGACATG
    AGGGATGAAG AGTTTTCCGC CAGTTCGGTT CTGGAGTCTG ATGTCATCCA TGCTAATAGG
    AAGGATATAC CCTGTATTTT TAGGGTAACA GCATCTCAGC TGTCCGCCTC CTCCAGTCAG
    AAGACGTGTA TTCTAATTCT GGCTGACAAC GAGCAAGAAA GGACGAAGTG GGTGTGCGCT
    CTGAATGAAC TGCATCGCAT CCTGAGGAAA AACAAGCTGA AGGAGCGTTT CGTCTATGTG
    CCTAAAGAAG CCTACGACAG CACACTTCCT CTCATCAAAA CCACCCAAAC TGCTGCTATT
    ATAGATCATG AACGCGTTGC TTTGGGCAAT GAGGAGGGCC TGTTTGTCAT CCACGTCACC
    AAAGATGAAA TCATTCGTGT GGGTGATGTC AAGAAGGTGC ATCATGTAGA ACTGATGCCC
    ACGGAGCACA TACTGGCCGT CATCTCGGGC AGGAACCGAC AAGTATGTCT GGTCTCCATG
    GCGGGTCTCG ACGGGAGGGA GGTCGACAAG AATAAGGTGG CAGATACTAA AAACTGCCAG
    TTGCTGGTGT CCGGTGTGGT TCGCAATGTC ACCTGCTTCT GCCTCGCCAT TAAACGGCAG
    ATCTCATGCT TCGAAATTAA CAAGAGCAAG TCACGACACT CCCATCTCGT GGACATACAA
    GCTCCAGGAA CTGTCCAATG GATGGCGCTG TTCAACCAGC AGTTGTGTGT TGGTTACCCA
    TCAGGCTTCA TGCGCTACAG TTTGTATGGA GAAACACCTC CAGCCAGCTT ACTTCATCCA
    GATGACCCCA CACTGGCATT TATTAAAACA GACAACCTGG ATGCCCTTTG TGCTGTGGAA
    ATCTCCAATA AGGAACTGCT GCTCTGCTTC AGTAAAATAG CAGTGTATGT TGACACACAC
    GGCAGACGCT CGCGCCAGCA GGAGCTGATG TGGCCCGCGG CGCCCACCGC CTGCTGCTAT
    AACGCCCCCT ATCTGTCGGT CTACAGTGAA AATGCAGTGG ATGTGTTTGA TGTGAACTCT
    ACGGAATGGA TTCAGACCAT TCCTCTCAAA AAGGTCCGGC CATTAAACAT GGATGGCTCT
    TTGAATCTCC TGGGCCTAGA AACGGTCAGA CTCATCTACT TCAGAAACAA GACCGCAGAG
    GGGGATGAAC TGGTGGTGCC TGAAGTGTGT GATAACAGCA GAAAGCAGAT GGTGCGAAGC
    ATGAGCAACA AGAGACGATT CTCGTTTCGT ATTCCTGAGG AGGAGAGACT GCAGCAGAGG
    AGAGAAATGT TGAAAGATCC TGAGAGGAGG AACAAGCTGA TCTCAAATCC TACAAATTTT
    AACCATGTGG CTCATATGGG ACCTGGAGAT GGCATGCAGA TACTCAGAGA CCTGCCAATG
    AATGTACGTG CTCAGGACAA CCGTGCTGGA TTCAGCGGCT CCGTCAGCAT CCCCTCCATT
    ACAAAAAACA GAGCAGAACC AGGGCGATCC ATGAGCGCAA GCAGTGGCCT AGGAATGCGG
    TCGTCCTCTC AGAACGGCAG TGCTCTAAGG AGGGAGCTGT CCAGCGGCAG CTACAGCTCT
    AAACGGCAGA CCATGACTTC ACCCTCCGAG AGTTCGCTGT CATCAGGAGG AGGGATGGAC
    GTGGGGGAAG CGCCGCTCTC TCAGTTTGAC AGAGAGGACT CTGATTCGCC GCGGCACTCC
    ACTGCCTCCA ACAGCTCCAC CTTCAGCAGC CCGCCGAGCC CCGCCTCCCC ACACAAGACA
    AAGTCCCTCT CATTGGAGAG CACAGACCGC ACCGCCTGGG ACACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324161.1
    CDS1641..6968
    Translation

    Target ORF information:

    RefSeq Version XM_021468486.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468486.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGTCGTCT
    CAACCTAACG ATGAGGAGCG CTTCCAGTTC CCACTTCAGG TGACTGATGT GTCCGAGGAT
    GCTAAAGATC TTGTTCGGCG GCTGATCTGC AGCAGAGAAC ACCGGCTGGG CCAGAACGGT
    ATCGAAGACT TTAAGCAGCA CCCCTTTTTC ACAGGCATCG ACTGGGACAA CATCCGCACA
    TGCGAAGCCC CCTACATCCC TGAAGTCAGC AGTCCCACTG ACACCTCCAA CTTTGATGTG
    GATGACGACT GCCTCAAAAA CTGTGAGACG TTGCCTCCAC CTTCACACAC AGCATTTTCT
    GGACACCACC TCCCATTCGT AGGCTTCACC TACACGAGTA AATGCACGCT GTCAGATCGA
    GGGTGTTTGC GGGAATCGGT CGGACCTGCA CAGGTTGATG CTCATGTTCA GCGTAGCCTG
    GAGGAGAGCC TGGCCTCTGA AGCCTATGAG AGGAGAATTC GACGTTTAGA GCAGGAGAAA
    ACCGAACTTA CCCGTAAACT ACAGGAGTCT ACGCAAACGG TGCAAGCACT GCAGTACCCT
    GATTCAGACG CCCCAGTGAA TGCTAATAAA GAGGTAGAGA TAAGGAGTTT AAAGAGTGAG
    ATCGACATCC TGAAGAAACA GATAGCAGAC TCTGGTCAGA TGGAGCAGCA GTTGGAGAAC
    GCCAGCACAG CTAGAAGAGA CCTGGAAGAC TCGTCCAAGG TCGTCAGGAA CTTAGAGAAA
    CAGCTGAAGA GTGTCACACA AGAGAAAGAT GATCTGCACA AGGAGCTGCT GGGTTTGGCA
    GAGAAGGTGA AGGCTCAGGG GAAGGAGCTA AAGGATGCTC ACAGTCAAAA AAAGATGGCC
    ATAAAGGAGT TCGCTGAGCT GAATGAACAG CTGAGCGATG TTCGCACACA AAAGCAGCGC
    CTCAGCCGAC AGTTCAAAGA CAAGGAGGAG GAGATGGAAG GAGTGTCGCA GAAACTAGAG
    GCTCTACGGC TAGAGATACG CAAAGCCGAG AGAATGCGCA AAGAGTTGGA GGCCCAGGCT
    GAGGAACATG CAGCAGAAGC TCAGAAGGAG AGAAAGGTTC GAGAAAGAAC AGAACAGTAC
    AGCAAACAAC TAGAGGAGGA GCTCGAGGGC ATGAAGCAGT TGAAGCAGAC TGGGCCTCCT
    AACGGAGCTG CAAGCTCAGA CCAGCAGCAG GAGCTGATGC GCGTTCGGGC TGAACTGGAG
    AAGAAGAGCG TACAGTACGA GGAGGAGATG TGTCGCAGAG AGACGCTCCA CAGCAGTGAG
    ATGAAGAACC TCCAAAAAGA GCTACGGGAT GCCGAGGGTC AACACCTCAC ACATCAGAAA
    GAGATACTCG TGCTTAAAGA CAAACTGGAG AAAACTCGCA GAGAAAGTCA AGTCGAGCGA
    GAAGAATTTG TGTGCGAGTA CAAGCAGAAG TACGAGAGGG AAAAAGCCCA TCTGACAGAC
    GAAAACAAGA AGCTGAGTGC CGAAGTGGAG AAGTTGAACG GTCAGTTCGA GCAGCTGAGC
    AGCAGTCACC GGCAGCTGGA GGAGGAGATG AGAGAACTGG CTGATAAGAA GGAGAGTGTT
    GCCCACTGGG AGGCCCAGAT CACCGAGATC ATCCAGTGGG TGAGCGATGA GAAGGATGCC
    CGTGGTTACC TTCAAGCTCT GGCCACCAAA ATGACAGAGG AGCTGGAAGG GCTCAGAAAC
    ACCAGTTTGG GAGCCAGAGG AACGGACATG CCGTGGAAGA TGAGGCGTTT CGCCAAGCTG
    GACATGTCTG CGCGTCTGGA GCTGCAGTCG GCGCTGGATA CTGAGATCAG AGCCAAACAG
    TCCATCCAAG ACGAGCTTAA CAAAGTCAAA GCAACCAACA TTGCCACAGA ATGCAAATTG
    CAAGAATCCG AAAGTAGAAA CCAGGAGCTG CTGGGAGAAA TTGAGAGACT GAGGAAGGAG
    ACAGAGGAGC TAAGGCTGAG GAGAGGCGTG AAGCACCAGG ATTCCCAAAA TTCCTTTTTG
    GCTTTCCTCA ATGCGCCCAC TTCTGCACTG GATCAGTTTG ATCGCTCACC CTCTTTTGGT
    CCCGGAAGCA AAACCAGACG TCTTGACTCT GCAGATTTCA CCCCTTCCAA CACTCCATCC
    AGAGATGAAG ACGCAAGATC TCGCCGTATT TCACGCTCTC GCTCACCCTC CACCGCCAGT
    GACATGGAGC CTATAGAGTT GATTGACCAC ACATCACGTA GTCTTCAGAC CCCAACAGGC
    AGATCAGGCT ACGGGAGCTT GGGACACTCA TCACCTAAGC CCAAGGCACA CCAGTTTAGT
    GTAAAGTCCT TCAATGTTCC CACTAAATGT AACCAGTGCA CCTCTCTCAT GGTGGGACTC
    ATCCGTCAGG GCTGCACATG CGAAGCCTGC AGCTTCTCCT GTCATGTCAC TTGTGCTGAT
    AAAGCCCCTT CTGTGTGCCC CGTCGCACCA GACCAGACCA GGGGCAAACT GGGCATTGAC
    ACTCAGAGGG GCATTGGGAC TGCATATGAG GGGCATCTTA GGGTGCCGAA GCCCACTGGG
    GTGAAGAAGG GTTGGCAGAG GGTCTGGGCA GTGGTTTGTG ACTTCAAACT TTTTCTGTAT
    GAGCTTGGAG AAGGGAAAGG TGCTCAGCCT GGTGTTGTAG TAAACCAGGT CATTGACATG
    AGGGATGAAG AGTTTTCCGC CAGTTCGGTT CTGGAGTCTG ATGTCATCCA TGCTAATAGG
    AAGGATATAC CCTGTATTTT TAGGGTAACA GCATCTCAGC TGTCCGCCTC CTCCAGTCAG
    AAGACGTGTA TTCTAATTCT GGCTGACAAC GAGCAAGAAA GGACGAAGTG GGTGTGCGCT
    CTGAATGAAC TGCATCGCAT CCTGAGGAAA AACAAGCTGA AGGAGCGTTT CGTCTATGTG
    CCTAAAGAAG CCTACGACAG CACACTTCCT CTCATCAAAA CCACCCAAAC TGCTGCTATT
    ATAGATCATG AACGCGTTGC TTTGGGCAAT GAGGAGGGCC TGTTTGTCAT CCACGTCACC
    AAAGATGAAA TCATTCGTGT GGGTGATGTC AAGAAGGTGC ATCATGTAGA ACTGATGCCC
    ACGGAGCACA TACTGGCCGT CATCTCGGGC AGGAACCGAC AAGTATGTCT GGTCTCCATG
    GCGGGTCTCG ACGGGAGGGA GGTCGACAAG AATAAGGTGG CAGATACTAA AAACTGCCAG
    TTGCTGGTGT CCGGTGTGGT TCGCAATGTC ACCTGCTTCT GCCTCGCCAT TAAACGGCAG
    ATCTCATGCT TCGAAATTAA CAAGAGCAAG TCACGACACT CCCATCTCGT GGACATACAA
    GCTCCAGGAA CTGTCCAATG GATGGCGCTG TTCAACCAGC AGTTGTGTGT TGGTTACCCA
    TCAGGCTTCA TGCGCTACAG TTTGTATGGA GAAACACCTC CAGCCAGCTT ACTTCATCCA
    GATGACCCCA CACTGGCATT TATTAAAACA GACAACCTGG ATGCCCTTTG TGCTGTGGAA
    ATCTCCAATA AGGAACTGCT GCTCTGCTTC AGTAAAATAG CAGTGTATGT TGACACACAC
    GGCAGACGCT CGCGCCAGCA GGAGCTGATG TGGCCCGCGG CGCCCACCGC CTGCTGCTAT
    AACGCCCCCT ATCTGTCGGT CTACAGTGAA AATGCAGTGG ATGTGTTTGA TGTGAACTCT
    ACGGAATGGA TTCAGACCAT TCCTCTCAAA AAGGTCCGGC CATTAAACAT GGATGGCTCT
    TTGAATCTCC TGGGCCTAGA AACGGTCAGA CTCATCTACT TCAGAAACAA GACCGCAGAG
    GGGGATGAAC TGGTGGTGCC TGAAGTGTGT GATAACAGCA GAAAGCAGAT GGTGCGAAGC
    ATGAGCAACA AGAGACGATT CTCGTTTCGT ATTCCTGAGG AGGAGAGACT GCAGCAGAGG
    AGAGAAATGT TGAAAGATCC TGAGAGGAGG AACAAGCTGA TCTCAAATCC TACAAATTTT
    AACCATGTGG CTCATATGGG ACCTGGAGAT GGCATGCAGA TACTCAGAGA CCTGCCAATG
    AATGTACGTG CTCAGGACAA CCGTGCTGGA TTCAGCGGCT CCGTCAGCAT CCCCTCCATT
    ACAAAAAACA GAGCAGAACC AGGGCGATCC ATGAGCGCAA GCAGTGGCCT AGGAATGCGG
    TCGTCCTCTC AGAACGGCAG TGCTCTAAGG AGGGAGCTGT CCAGCGGCAG CTACAGCTCT
    AAACGGCAGA CCATGACTTC ACCCTCCGAG AGTTCGCTGT CATCAGGAGG AGGGATGGAC
    GTGGGGGAAG CGCCGCTCTC TCAGTTTGAC AGAGAGGACT CTGATTCGCC GCGGCACTCC
    ACTGCCTCCA ACAGCTCCAC CTTCAGCAGC CCGCCGAGCC CCGCCTCCCC ACACAAGACA
    AAGTCCCTCT CATTGGAGAG CACAGACCGC ACCGCCTGGG ACACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56577
    Clone ID Related Accession (Same CDS sequence) XM_017352506.2 , XM_017352506.1
    Accession Version XM_017352506.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5379bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase MRCK alpha isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352506.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC
    TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG
    CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC
    CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT
    GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC
    TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA
    GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC
    GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA
    GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA
    CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT
    GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG
    ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC
    CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA
    GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG
    AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG
    AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC
    AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC
    AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT
    CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG
    CTCGAGGGCA TGAAGTTGAA GCAGACTGGG CCTCCTAACG GAGCTGCAAG CTCAGACCAG
    CAGCAGGAGC TGATGCGCGT TCGGGCTGAA CTGGAGAAGA AGAGCGTACA GTACGAGGAG
    GAGATGTGTC GCAGAGAGAC GCTCCACAGC AGTGAGATGA AGAACCTCCA AAAAGAGCTA
    CGGGATGCCG AGGGTCAACA CCTCACACAT CAGAAAGAGA TACTCGTGCT TAAAGACAAA
    CTGGAGAAAA CTCGCAGAGA AAGTCAAGTC GAGCGAGAAG AATTTGTGTG CGAGTACAAG
    CAGAAGTACG AGAGGGAAAA AGCCCATCTG ACAGACGAAA ACAAGAAGCT GAGTGCCGAA
    GTGGAGAAGT TGAACGGTCA GTTCGAGCAG CTGAGCAGCA GTCACCGGCA GCTGGAGGAG
    GAGATGAGAG AACTGGCTGA TAAGAAGGAG AGTGTTGCCC ACTGGGAGGC CCAGATCACC
    GAGATCATCC AGTGGGTGAG CGATGAGAAG GATGCCCGTG GTTACCTTCA AGCTCTGGCC
    ACCAAAATGA CAGAGGAGCT GGAAGGGCTC AGAAACACCA GTTTGGGAGC CAGAGGAACG
    GACATGCCGT GGAAGATGAG GCGTTTCGCC AAGCTGGACA TGTCTGCGCG TCTGGAGCTG
    CAGTCGGCGC TGGATACTGA GATCAGAGCC AAACAGTCCA TCCAAGACGA GCTTAACAAA
    GTCAAAGCAA CCAACATTGC CACAGAATGC AAATTGCAAG AATCCGAAAG TAGAAACCAG
    GAGCTGCTGG GAGAAATTGA GAGACTGAGG AAGGAGACAG AGGAGCTAAG GCTGAGGAGA
    GGCGTGAAGC ACCAGGATTC CCAAAATTCC TTTTTGGCTT TCCTCAATGC GCCCACTTCT
    GCACTGGATC AGTTTGATCG CTCACCCTCT TTTGGTCCCG GAAGCAAAAC CAGACGTCTT
    GACTCTGCAG ATTTCACCCC TTCCAACACT CCATCCAGAG ATGAAGACGC AAGATCTCGC
    CGTATTTCAC GCTCTCGCTC ACCCTCCACC GCCAGTGACA TGGAGCCTAT AGAGTTGATT
    GACCACACAT CACGTAGTCT TCAGACCCCA ACAGGCAGAT CAGGCTACGG GAGCTTGGGA
    CACTCATCAC CTAAGCCCAA GGCACACCAG TTTAGTGTAA AGTCCTTCAA TGTTCCCACT
    AAATGTAACC AGTGCACCTC TCTCATGGTG GGACTCATCC GTCAGGGCTG CACATGCGAA
    GCCTGCAGCT TCTCCTGTCA TGTCACTTGT GCTGATAAAG CCCCTTCTGT GTGCCCCGTC
    GCACCAGACC AGACCAGGGG CAAACTGGGC ATTGACACTC AGAGGGGCAT TGGGACTGCA
    TATGAGGGGC ATCTTAGGGT GCCGAAGCCC ACTGGGGTGA AGAAGGGTTG GCAGAGGGTC
    TGGGCAGTGG TTTGTGACTT CAAACTTTTT CTGTATGAGC TTGGAGAAGG GAAAGGTGCT
    CAGCCTGGTG TTGTAGTAAA CCAGGTCATT GACATGAGGG ATGAAGAGTT TTCCGCCAGT
    TCGGTTCTGG AGTCTGATGT CATCCATGCT AATAGGAAGG ATATACCCTG TATTTTTAGG
    GTAACAGCAT CTCAGCTGTC CGCCTCCTCC AGTCAGAAGA CGTGTATTCT AATTCTGGCT
    GACAACGAGC AAGAAAGGAC GAAGTGGGTG TGCGCTCTGA ATGAACTGCA TCGCATCCTG
    AGGAAAAACA AGCTGAAGGA GCGTTTCGTC TATGTGCCTA AAGAAGCCTA CGACAGCACA
    CTTCCTCTCA TCAAAACCAC CCAAACTGCT GCTATTATAG ATCATGAACG CGTTGCTTTG
    GGCAATGAGG AGGGCCTGTT TGTCATCCAC GTCACCAAAG ATGAAATCAT TCGTGTGGGT
    GATGTCAAGA AGGTGCATCA TGTAGAACTG ATGCCCACGG AGCACATACT GGCCGTCATC
    TCGGGCAGGA ACCGACAAGT ATGTCTGGTC TCCATGGCGG GTCTCGACGG GAGGGAGGTC
    GACAAGAATA AGGTGGCAGA TACTAAAAAC TGCCAGTTGC TGGTGTCCGG TGTGGTTCGC
    AATGTCACCT GCTTCTGCCT CGCCATTAAA CGGCAGATCT CATGCTTCGA AATTAACAAG
    AGCAAGTCAC GACACTCCCA TCTCGTGGAC ATACAAGCTC CAGGAACTGT CCAATGGATG
    GCGCTGTTCA ACCAGCAGTT GTGTGTTGGT TACCCATCAG GCTTCATGCG CTACAGTTTG
    TATGGAGAAA CACCTCCAGC CAGCTTACTT CATCCAGATG ACCCCACACT GGCATTTATT
    AAAACAGACA ACCTGGATGC CCTTTGTGCT GTGGAAATCT CCAATAAGGA ACTGCTGCTC
    TGCTTCAGTA AAATAGCAGT GTATGTTGAC ACACACGGCA GACGCTCGCG CCAGCAGGAG
    CTGATGTGGC CCGCGGCGCC CACCGCCTGC TGCTATAACG CCCCCTATCT GTCGGTCTAC
    AGTGAAAATG CAGTGGATGT GTTTGATGTG AACTCTACGG AATGGATTCA GACCATTCCT
    CTCAAAAAGG TCCGGCCATT AAACATGGAT GGCTCTTTGA ATCTCCTGGG CCTAGAAACG
    GTCAGACTCA TCTACTTCAG AAACAAGACC GCAGAGGGGG ATGAACTGGT GGTGCCTGAA
    GTGTGTGATA ACAGCAGAAA GCAGATGGTG CGAAGCATGA GCAACAAGAG ACGATTCTCG
    TTTCGTATTC CTGAGGAGGA GAGACTGCAG CAGAGGAGAG AAATGTTGAA AGATCCTGAG
    AGGAGGAACA AGCTGATCTC AAATCCTACA AATTTTAACC ATGTGGCTCA TATGGGACCT
    GGAGATGGCA TGCAGATACT CAGAGACCTG CCAATGAATG TACGTGCTCA GGACAACCGT
    GCTGGATTCA GCGGCTCCGT CAGCATCCCC TCCATTACAA AAAACAGAGC AGAACCAGGG
    CGATCCATGA GCGCAAGCAG TGGCCTAGGA ATGCGGTCGT CCTCTCAGAA CGGCAGTGCT
    CTAAGGAGGG AGCTGTCCAG CGGCAGCTAC AGCTCTAAAC GGCAGACCAT GACTTCACCC
    TCCGAGAGTT CGCTGTCATC AGGAGGAGGG ATGGACGTGG GGGAAGCGCC GCTCTCTCAG
    TTTGACAGAG AGTGGCAGGC AGTATCGCCC TCGGAGAAGA CCCCTGATCT GAAAAGGGCT
    TTAAGGACCC TGCTTAGTAA GATGAAGGAC TCTGATTCGC CGCGGCACTC CACTGCCTCC
    AACAGCTCCA CCTTCAGCAG CCCGCCGAGC CCCGCCTCCC CACACAAGAC AAAGTCCCTC
    TCATTGGAGA GCACAGACCG CACCGCCTGG GACACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207995.1
    CDS1641..7019
    Translation

    Target ORF information:

    RefSeq Version XM_017352506.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352506.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC
    TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG
    CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC
    CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT
    GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC
    TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA
    GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC
    GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA
    GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA
    CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT
    GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG
    ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC
    CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA
    GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG
    AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG
    AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC
    AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC
    AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT
    CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG
    CTCGAGGGCA TGAAGTTGAA GCAGACTGGG CCTCCTAACG GAGCTGCAAG CTCAGACCAG
    CAGCAGGAGC TGATGCGCGT TCGGGCTGAA CTGGAGAAGA AGAGCGTACA GTACGAGGAG
    GAGATGTGTC GCAGAGAGAC GCTCCACAGC AGTGAGATGA AGAACCTCCA AAAAGAGCTA
    CGGGATGCCG AGGGTCAACA CCTCACACAT CAGAAAGAGA TACTCGTGCT TAAAGACAAA
    CTGGAGAAAA CTCGCAGAGA AAGTCAAGTC GAGCGAGAAG AATTTGTGTG CGAGTACAAG
    CAGAAGTACG AGAGGGAAAA AGCCCATCTG ACAGACGAAA ACAAGAAGCT GAGTGCCGAA
    GTGGAGAAGT TGAACGGTCA GTTCGAGCAG CTGAGCAGCA GTCACCGGCA GCTGGAGGAG
    GAGATGAGAG AACTGGCTGA TAAGAAGGAG AGTGTTGCCC ACTGGGAGGC CCAGATCACC
    GAGATCATCC AGTGGGTGAG CGATGAGAAG GATGCCCGTG GTTACCTTCA AGCTCTGGCC
    ACCAAAATGA CAGAGGAGCT GGAAGGGCTC AGAAACACCA GTTTGGGAGC CAGAGGAACG
    GACATGCCGT GGAAGATGAG GCGTTTCGCC AAGCTGGACA TGTCTGCGCG TCTGGAGCTG
    CAGTCGGCGC TGGATACTGA GATCAGAGCC AAACAGTCCA TCCAAGACGA GCTTAACAAA
    GTCAAAGCAA CCAACATTGC CACAGAATGC AAATTGCAAG AATCCGAAAG TAGAAACCAG
    GAGCTGCTGG GAGAAATTGA GAGACTGAGG AAGGAGACAG AGGAGCTAAG GCTGAGGAGA
    GGCGTGAAGC ACCAGGATTC CCAAAATTCC TTTTTGGCTT TCCTCAATGC GCCCACTTCT
    GCACTGGATC AGTTTGATCG CTCACCCTCT TTTGGTCCCG GAAGCAAAAC CAGACGTCTT
    GACTCTGCAG ATTTCACCCC TTCCAACACT CCATCCAGAG ATGAAGACGC AAGATCTCGC
    CGTATTTCAC GCTCTCGCTC ACCCTCCACC GCCAGTGACA TGGAGCCTAT AGAGTTGATT
    GACCACACAT CACGTAGTCT TCAGACCCCA ACAGGCAGAT CAGGCTACGG GAGCTTGGGA
    CACTCATCAC CTAAGCCCAA GGCACACCAG TTTAGTGTAA AGTCCTTCAA TGTTCCCACT
    AAATGTAACC AGTGCACCTC TCTCATGGTG GGACTCATCC GTCAGGGCTG CACATGCGAA
    GCCTGCAGCT TCTCCTGTCA TGTCACTTGT GCTGATAAAG CCCCTTCTGT GTGCCCCGTC
    GCACCAGACC AGACCAGGGG CAAACTGGGC ATTGACACTC AGAGGGGCAT TGGGACTGCA
    TATGAGGGGC ATCTTAGGGT GCCGAAGCCC ACTGGGGTGA AGAAGGGTTG GCAGAGGGTC
    TGGGCAGTGG TTTGTGACTT CAAACTTTTT CTGTATGAGC TTGGAGAAGG GAAAGGTGCT
    CAGCCTGGTG TTGTAGTAAA CCAGGTCATT GACATGAGGG ATGAAGAGTT TTCCGCCAGT
    TCGGTTCTGG AGTCTGATGT CATCCATGCT AATAGGAAGG ATATACCCTG TATTTTTAGG
    GTAACAGCAT CTCAGCTGTC CGCCTCCTCC AGTCAGAAGA CGTGTATTCT AATTCTGGCT
    GACAACGAGC AAGAAAGGAC GAAGTGGGTG TGCGCTCTGA ATGAACTGCA TCGCATCCTG
    AGGAAAAACA AGCTGAAGGA GCGTTTCGTC TATGTGCCTA AAGAAGCCTA CGACAGCACA
    CTTCCTCTCA TCAAAACCAC CCAAACTGCT GCTATTATAG ATCATGAACG CGTTGCTTTG
    GGCAATGAGG AGGGCCTGTT TGTCATCCAC GTCACCAAAG ATGAAATCAT TCGTGTGGGT
    GATGTCAAGA AGGTGCATCA TGTAGAACTG ATGCCCACGG AGCACATACT GGCCGTCATC
    TCGGGCAGGA ACCGACAAGT ATGTCTGGTC TCCATGGCGG GTCTCGACGG GAGGGAGGTC
    GACAAGAATA AGGTGGCAGA TACTAAAAAC TGCCAGTTGC TGGTGTCCGG TGTGGTTCGC
    AATGTCACCT GCTTCTGCCT CGCCATTAAA CGGCAGATCT CATGCTTCGA AATTAACAAG
    AGCAAGTCAC GACACTCCCA TCTCGTGGAC ATACAAGCTC CAGGAACTGT CCAATGGATG
    GCGCTGTTCA ACCAGCAGTT GTGTGTTGGT TACCCATCAG GCTTCATGCG CTACAGTTTG
    TATGGAGAAA CACCTCCAGC CAGCTTACTT CATCCAGATG ACCCCACACT GGCATTTATT
    AAAACAGACA ACCTGGATGC CCTTTGTGCT GTGGAAATCT CCAATAAGGA ACTGCTGCTC
    TGCTTCAGTA AAATAGCAGT GTATGTTGAC ACACACGGCA GACGCTCGCG CCAGCAGGAG
    CTGATGTGGC CCGCGGCGCC CACCGCCTGC TGCTATAACG CCCCCTATCT GTCGGTCTAC
    AGTGAAAATG CAGTGGATGT GTTTGATGTG AACTCTACGG AATGGATTCA GACCATTCCT
    CTCAAAAAGG TCCGGCCATT AAACATGGAT GGCTCTTTGA ATCTCCTGGG CCTAGAAACG
    GTCAGACTCA TCTACTTCAG AAACAAGACC GCAGAGGGGG ATGAACTGGT GGTGCCTGAA
    GTGTGTGATA ACAGCAGAAA GCAGATGGTG CGAAGCATGA GCAACAAGAG ACGATTCTCG
    TTTCGTATTC CTGAGGAGGA GAGACTGCAG CAGAGGAGAG AAATGTTGAA AGATCCTGAG
    AGGAGGAACA AGCTGATCTC AAATCCTACA AATTTTAACC ATGTGGCTCA TATGGGACCT
    GGAGATGGCA TGCAGATACT CAGAGACCTG CCAATGAATG TACGTGCTCA GGACAACCGT
    GCTGGATTCA GCGGCTCCGT CAGCATCCCC TCCATTACAA AAAACAGAGC AGAACCAGGG
    CGATCCATGA GCGCAAGCAG TGGCCTAGGA ATGCGGTCGT CCTCTCAGAA CGGCAGTGCT
    CTAAGGAGGG AGCTGTCCAG CGGCAGCTAC AGCTCTAAAC GGCAGACCAT GACTTCACCC
    TCCGAGAGTT CGCTGTCATC AGGAGGAGGG ATGGACGTGG GGGAAGCGCC GCTCTCTCAG
    TTTGACAGAG AGTGGCAGGC AGTATCGCCC TCGGAGAAGA CCCCTGATCT GAAAAGGGCT
    TTAAGGACCC TGCTTAGTAA GATGAAGGAC TCTGATTCGC CGCGGCACTC CACTGCCTCC
    AACAGCTCCA CCTTCAGCAG CCCGCCGAGC CCCGCCTCCC CACACAAGAC AAAGTCCCTC
    TCATTGGAGA GCACAGACCG CACCGCCTGG GACACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56577
    Clone ID Related Accession (Same CDS sequence) XM_017352506.2 , XM_017352506.1
    Accession Version XM_017352506.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5379bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product serine/threonine-protein kinase MRCK alpha isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC
    TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG
    CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC
    CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT
    GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC
    TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA
    GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC
    GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA
    GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA
    CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT
    GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG
    ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC
    CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA
    GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG
    AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG
    AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC
    AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC
    AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT
    CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG
    CTCGAGGGCA TGAAGTTGAA GCAGACTGGG CCTCCTAACG GAGCTGCAAG CTCAGACCAG
    CAGCAGGAGC TGATGCGCGT TCGGGCTGAA CTGGAGAAGA AGAGCGTACA GTACGAGGAG
    GAGATGTGTC GCAGAGAGAC GCTCCACAGC AGTGAGATGA AGAACCTCCA AAAAGAGCTA
    CGGGATGCCG AGGGTCAACA CCTCACACAT CAGAAAGAGA TACTCGTGCT TAAAGACAAA
    CTGGAGAAAA CTCGCAGAGA AAGTCAAGTC GAGCGAGAAG AATTTGTGTG CGAGTACAAG
    CAGAAGTACG AGAGGGAAAA AGCCCATCTG ACAGACGAAA ACAAGAAGCT GAGTGCCGAA
    GTGGAGAAGT TGAACGGTCA GTTCGAGCAG CTGAGCAGCA GTCACCGGCA GCTGGAGGAG
    GAGATGAGAG AACTGGCTGA TAAGAAGGAG AGTGTTGCCC ACTGGGAGGC CCAGATCACC
    GAGATCATCC AGTGGGTGAG CGATGAGAAG GATGCCCGTG GTTACCTTCA AGCTCTGGCC
    ACCAAAATGA CAGAGGAGCT GGAAGGGCTC AGAAACACCA GTTTGGGAGC CAGAGGAACG
    GACATGCCGT GGAAGATGAG GCGTTTCGCC AAGCTGGACA TGTCTGCGCG TCTGGAGCTG
    CAGTCGGCGC TGGATACTGA GATCAGAGCC AAACAGTCCA TCCAAGACGA GCTTAACAAA
    GTCAAAGCAA CCAACATTGC CACAGAATGC AAATTGCAAG AATCCGAAAG TAGAAACCAG
    GAGCTGCTGG GAGAAATTGA GAGACTGAGG AAGGAGACAG AGGAGCTAAG GCTGAGGAGA
    GGCGTGAAGC ACCAGGATTC CCAAAATTCC TTTTTGGCTT TCCTCAATGC GCCCACTTCT
    GCACTGGATC AGTTTGATCG CTCACCCTCT TTTGGTCCCG GAAGCAAAAC CAGACGTCTT
    GACTCTGCAG ATTTCACCCC TTCCAACACT CCATCCAGAG ATGAAGACGC AAGATCTCGC
    CGTATTTCAC GCTCTCGCTC ACCCTCCACC GCCAGTGACA TGGAGCCTAT AGAGTTGATT
    GACCACACAT CACGTAGTCT TCAGACCCCA ACAGGCAGAT CAGGCTACGG GAGCTTGGGA
    CACTCATCAC CTAAGCCCAA GGCACACCAG TTTAGTGTAA AGTCCTTCAA TGTTCCCACT
    AAATGTAACC AGTGCACCTC TCTCATGGTG GGACTCATCC GTCAGGGCTG CACATGCGAA
    GCCTGCAGCT TCTCCTGTCA TGTCACTTGT GCTGATAAAG CCCCTTCTGT GTGCCCCGTC
    GCACCAGACC AGACCAGGGG CAAACTGGGC ATTGACACTC AGAGGGGCAT TGGGACTGCA
    TATGAGGGGC ATCTTAGGGT GCCGAAGCCC ACTGGGGTGA AGAAGGGTTG GCAGAGGGTC
    TGGGCAGTGG TTTGTGACTT CAAACTTTTT CTGTATGAGC TTGGAGAAGG GAAAGGTGCT
    CAGCCTGGTG TTGTAGTAAA CCAGGTCATT GACATGAGGG ATGAAGAGTT TTCCGCCAGT
    TCGGTTCTGG AGTCTGATGT CATCCATGCT AATAGGAAGG ATATACCCTG TATTTTTAGG
    GTAACAGCAT CTCAGCTGTC CGCCTCCTCC AGTCAGAAGA CGTGTATTCT AATTCTGGCT
    GACAACGAGC AAGAAAGGAC GAAGTGGGTG TGCGCTCTGA ATGAACTGCA TCGCATCCTG
    AGGAAAAACA AGCTGAAGGA GCGTTTCGTC TATGTGCCTA AAGAAGCCTA CGACAGCACA
    CTTCCTCTCA TCAAAACCAC CCAAACTGCT GCTATTATAG ATCATGAACG CGTTGCTTTG
    GGCAATGAGG AGGGCCTGTT TGTCATCCAC GTCACCAAAG ATGAAATCAT TCGTGTGGGT
    GATGTCAAGA AGGTGCATCA TGTAGAACTG ATGCCCACGG AGCACATACT GGCCGTCATC
    TCGGGCAGGA ACCGACAAGT ATGTCTGGTC TCCATGGCGG GTCTCGACGG GAGGGAGGTC
    GACAAGAATA AGGTGGCAGA TACTAAAAAC TGCCAGTTGC TGGTGTCCGG TGTGGTTCGC
    AATGTCACCT GCTTCTGCCT CGCCATTAAA CGGCAGATCT CATGCTTCGA AATTAACAAG
    AGCAAGTCAC GACACTCCCA TCTCGTGGAC ATACAAGCTC CAGGAACTGT CCAATGGATG
    GCGCTGTTCA ACCAGCAGTT GTGTGTTGGT TACCCATCAG GCTTCATGCG CTACAGTTTG
    TATGGAGAAA CACCTCCAGC CAGCTTACTT CATCCAGATG ACCCCACACT GGCATTTATT
    AAAACAGACA ACCTGGATGC CCTTTGTGCT GTGGAAATCT CCAATAAGGA ACTGCTGCTC
    TGCTTCAGTA AAATAGCAGT GTATGTTGAC ACACACGGCA GACGCTCGCG CCAGCAGGAG
    CTGATGTGGC CCGCGGCGCC CACCGCCTGC TGCTATAACG CCCCCTATCT GTCGGTCTAC
    AGTGAAAATG CAGTGGATGT GTTTGATGTG AACTCTACGG AATGGATTCA GACCATTCCT
    CTCAAAAAGG TCCGGCCATT AAACATGGAT GGCTCTTTGA ATCTCCTGGG CCTAGAAACG
    GTCAGACTCA TCTACTTCAG AAACAAGACC GCAGAGGGGG ATGAACTGGT GGTGCCTGAA
    GTGTGTGATA ACAGCAGAAA GCAGATGGTG CGAAGCATGA GCAACAAGAG ACGATTCTCG
    TTTCGTATTC CTGAGGAGGA GAGACTGCAG CAGAGGAGAG AAATGTTGAA AGATCCTGAG
    AGGAGGAACA AGCTGATCTC AAATCCTACA AATTTTAACC ATGTGGCTCA TATGGGACCT
    GGAGATGGCA TGCAGATACT CAGAGACCTG CCAATGAATG TACGTGCTCA GGACAACCGT
    GCTGGATTCA GCGGCTCCGT CAGCATCCCC TCCATTACAA AAAACAGAGC AGAACCAGGG
    CGATCCATGA GCGCAAGCAG TGGCCTAGGA ATGCGGTCGT CCTCTCAGAA CGGCAGTGCT
    CTAAGGAGGG AGCTGTCCAG CGGCAGCTAC AGCTCTAAAC GGCAGACCAT GACTTCACCC
    TCCGAGAGTT CGCTGTCATC AGGAGGAGGG ATGGACGTGG GGGAAGCGCC GCTCTCTCAG
    TTTGACAGAG AGTGGCAGGC AGTATCGCCC TCGGAGAAGA CCCCTGATCT GAAAAGGGCT
    TTAAGGACCC TGCTTAGTAA GATGAAGGAC TCTGATTCGC CGCGGCACTC CACTGCCTCC
    AACAGCTCCA CCTTCAGCAG CCCGCCGAGC CCCGCCTCCC CACACAAGAC AAAGTCCCTC
    TCATTGGAGA GCACAGACCG CACCGCCTGG GACACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207995.1
    CDS1650..7028
    Translation

    Target ORF information:

    RefSeq Version XM_017352506.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352506.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC
    TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG
    CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC
    CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT
    GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC
    TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA
    GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC
    GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA
    GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA
    CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT
    GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG
    ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC
    CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA
    GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG
    AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG
    AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC
    AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC
    AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT
    CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG
    CTCGAGGGCA TGAAGTTGAA GCAGACTGGG CCTCCTAACG GAGCTGCAAG CTCAGACCAG
    CAGCAGGAGC TGATGCGCGT TCGGGCTGAA CTGGAGAAGA AGAGCGTACA GTACGAGGAG
    GAGATGTGTC GCAGAGAGAC GCTCCACAGC AGTGAGATGA AGAACCTCCA AAAAGAGCTA
    CGGGATGCCG AGGGTCAACA CCTCACACAT CAGAAAGAGA TACTCGTGCT TAAAGACAAA
    CTGGAGAAAA CTCGCAGAGA AAGTCAAGTC GAGCGAGAAG AATTTGTGTG CGAGTACAAG
    CAGAAGTACG AGAGGGAAAA AGCCCATCTG ACAGACGAAA ACAAGAAGCT GAGTGCCGAA
    GTGGAGAAGT TGAACGGTCA GTTCGAGCAG CTGAGCAGCA GTCACCGGCA GCTGGAGGAG
    GAGATGAGAG AACTGGCTGA TAAGAAGGAG AGTGTTGCCC ACTGGGAGGC CCAGATCACC
    GAGATCATCC AGTGGGTGAG CGATGAGAAG GATGCCCGTG GTTACCTTCA AGCTCTGGCC
    ACCAAAATGA CAGAGGAGCT GGAAGGGCTC AGAAACACCA GTTTGGGAGC CAGAGGAACG
    GACATGCCGT GGAAGATGAG GCGTTTCGCC AAGCTGGACA TGTCTGCGCG TCTGGAGCTG
    CAGTCGGCGC TGGATACTGA GATCAGAGCC AAACAGTCCA TCCAAGACGA GCTTAACAAA
    GTCAAAGCAA CCAACATTGC CACAGAATGC AAATTGCAAG AATCCGAAAG TAGAAACCAG
    GAGCTGCTGG GAGAAATTGA GAGACTGAGG AAGGAGACAG AGGAGCTAAG GCTGAGGAGA
    GGCGTGAAGC ACCAGGATTC CCAAAATTCC TTTTTGGCTT TCCTCAATGC GCCCACTTCT
    GCACTGGATC AGTTTGATCG CTCACCCTCT TTTGGTCCCG GAAGCAAAAC CAGACGTCTT
    GACTCTGCAG ATTTCACCCC TTCCAACACT CCATCCAGAG ATGAAGACGC AAGATCTCGC
    CGTATTTCAC GCTCTCGCTC ACCCTCCACC GCCAGTGACA TGGAGCCTAT AGAGTTGATT
    GACCACACAT CACGTAGTCT TCAGACCCCA ACAGGCAGAT CAGGCTACGG GAGCTTGGGA
    CACTCATCAC CTAAGCCCAA GGCACACCAG TTTAGTGTAA AGTCCTTCAA TGTTCCCACT
    AAATGTAACC AGTGCACCTC TCTCATGGTG GGACTCATCC GTCAGGGCTG CACATGCGAA
    GCCTGCAGCT TCTCCTGTCA TGTCACTTGT GCTGATAAAG CCCCTTCTGT GTGCCCCGTC
    GCACCAGACC AGACCAGGGG CAAACTGGGC ATTGACACTC AGAGGGGCAT TGGGACTGCA
    TATGAGGGGC ATCTTAGGGT GCCGAAGCCC ACTGGGGTGA AGAAGGGTTG GCAGAGGGTC
    TGGGCAGTGG TTTGTGACTT CAAACTTTTT CTGTATGAGC TTGGAGAAGG GAAAGGTGCT
    CAGCCTGGTG TTGTAGTAAA CCAGGTCATT GACATGAGGG ATGAAGAGTT TTCCGCCAGT
    TCGGTTCTGG AGTCTGATGT CATCCATGCT AATAGGAAGG ATATACCCTG TATTTTTAGG
    GTAACAGCAT CTCAGCTGTC CGCCTCCTCC AGTCAGAAGA CGTGTATTCT AATTCTGGCT
    GACAACGAGC AAGAAAGGAC GAAGTGGGTG TGCGCTCTGA ATGAACTGCA TCGCATCCTG
    AGGAAAAACA AGCTGAAGGA GCGTTTCGTC TATGTGCCTA AAGAAGCCTA CGACAGCACA
    CTTCCTCTCA TCAAAACCAC CCAAACTGCT GCTATTATAG ATCATGAACG CGTTGCTTTG
    GGCAATGAGG AGGGCCTGTT TGTCATCCAC GTCACCAAAG ATGAAATCAT TCGTGTGGGT
    GATGTCAAGA AGGTGCATCA TGTAGAACTG ATGCCCACGG AGCACATACT GGCCGTCATC
    TCGGGCAGGA ACCGACAAGT ATGTCTGGTC TCCATGGCGG GTCTCGACGG GAGGGAGGTC
    GACAAGAATA AGGTGGCAGA TACTAAAAAC TGCCAGTTGC TGGTGTCCGG TGTGGTTCGC
    AATGTCACCT GCTTCTGCCT CGCCATTAAA CGGCAGATCT CATGCTTCGA AATTAACAAG
    AGCAAGTCAC GACACTCCCA TCTCGTGGAC ATACAAGCTC CAGGAACTGT CCAATGGATG
    GCGCTGTTCA ACCAGCAGTT GTGTGTTGGT TACCCATCAG GCTTCATGCG CTACAGTTTG
    TATGGAGAAA CACCTCCAGC CAGCTTACTT CATCCAGATG ACCCCACACT GGCATTTATT
    AAAACAGACA ACCTGGATGC CCTTTGTGCT GTGGAAATCT CCAATAAGGA ACTGCTGCTC
    TGCTTCAGTA AAATAGCAGT GTATGTTGAC ACACACGGCA GACGCTCGCG CCAGCAGGAG
    CTGATGTGGC CCGCGGCGCC CACCGCCTGC TGCTATAACG CCCCCTATCT GTCGGTCTAC
    AGTGAAAATG CAGTGGATGT GTTTGATGTG AACTCTACGG AATGGATTCA GACCATTCCT
    CTCAAAAAGG TCCGGCCATT AAACATGGAT GGCTCTTTGA ATCTCCTGGG CCTAGAAACG
    GTCAGACTCA TCTACTTCAG AAACAAGACC GCAGAGGGGG ATGAACTGGT GGTGCCTGAA
    GTGTGTGATA ACAGCAGAAA GCAGATGGTG CGAAGCATGA GCAACAAGAG ACGATTCTCG
    TTTCGTATTC CTGAGGAGGA GAGACTGCAG CAGAGGAGAG AAATGTTGAA AGATCCTGAG
    AGGAGGAACA AGCTGATCTC AAATCCTACA AATTTTAACC ATGTGGCTCA TATGGGACCT
    GGAGATGGCA TGCAGATACT CAGAGACCTG CCAATGAATG TACGTGCTCA GGACAACCGT
    GCTGGATTCA GCGGCTCCGT CAGCATCCCC TCCATTACAA AAAACAGAGC AGAACCAGGG
    CGATCCATGA GCGCAAGCAG TGGCCTAGGA ATGCGGTCGT CCTCTCAGAA CGGCAGTGCT
    CTAAGGAGGG AGCTGTCCAG CGGCAGCTAC AGCTCTAAAC GGCAGACCAT GACTTCACCC
    TCCGAGAGTT CGCTGTCATC AGGAGGAGGG ATGGACGTGG GGGAAGCGCC GCTCTCTCAG
    TTTGACAGAG AGTGGCAGGC AGTATCGCCC TCGGAGAAGA CCCCTGATCT GAAAAGGGCT
    TTAAGGACCC TGCTTAGTAA GATGAAGGAC TCTGATTCGC CGCGGCACTC CACTGCCTCC
    AACAGCTCCA CCTTCAGCAG CCCGCCGAGC CCCGCCTCCC CACACAAGAC AAAGTCCCTC
    TCATTGGAGA GCACAGACCG CACCGCCTGG GACACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56578
    Clone ID Related Accession (Same CDS sequence) XM_017352507.1 , XM_017352507.2
    Accession Version XM_017352507.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5304bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product serine/threonine-protein kinase MRCK alpha isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC
    TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG
    CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC
    CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT
    GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC
    TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA
    GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC
    GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA
    GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA
    CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT
    GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG
    ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC
    CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA
    GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG
    AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG
    AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC
    AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC
    AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT
    CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG
    CTCGAGGGCA TGAAGTTGAA GCAGACTGGG CCTCCTAACG GAGCTGCAAG CTCAGACCAG
    CAGCAGGAGC TGATGCGCGT TCGGGCTGAA CTGGAGAAGA AGAGCGTACA GTACGAGGAG
    GAGATGTGTC GCAGAGAGAC GCTCCACAGC AGTGAGATGA AGAACCTCCA AAAAGAGCTA
    CGGGATGCCG AGGGTCAACA CCTCACACAT CAGAAAGAGA TACTCGTGCT TAAAGACAAA
    CTGGAGAAAA CTCGCAGAGA AAGTCAAGTC GAGCGAGAAG AATTTGTGTG CGAGTACAAG
    CAGAAGTACG AGAGGGAAAA AGCCCATCTG ACAGACGAAA ACAAGAAGCT GAGTGCCGAA
    GTGGAGAAGT TGAACGGTCA GTTCGAGCAG CTGAGCAGCA GTCACCGGCA GCTGGAGGAG
    GAGATGAGAG AACTGGCTGA TAAGAAGGAG AGTGTTGCCC ACTGGGAGGC CCAGATCACC
    GAGATCATCC AGTGGGTGAG CGATGAGAAG GATGCCCGTG GTTACCTTCA AGCTCTGGCC
    ACCAAAATGA CAGAGGAGCT GGAAGGGCTC AGAAACACCA GTTTGGGAGC CAGAGGAACG
    GACATGCCGT GGAAGATGAG GCGTTTCGCC AAGCTGGACA TGTCTGCGCG TCTGGAGCTG
    CAGTCGGCGC TGGATACTGA GATCAGAGCC AAACAGTCCA TCCAAGACGA GCTTAACAAA
    GTCAAAGCAA CCAACATTGC CACAGAATGC AAATTGCAAG AATCCGAAAG TAGAAACCAG
    GAGCTGCTGG GAGAAATTGA GAGACTGAGG AAGGAGACAG AGGAGCTAAG GCTGAGGAGA
    GGCGTGAAGC ACCAGGATTC CCAAAATTCC TTTTTGGCTT TCCTCAATGC GCCCACTTCT
    GCACTGGATC AGTTTGATCG CTCACCCTCT TTTGGTCCCG GAAGCAAAAC CAGACGTCTT
    GACTCTGCAG ATTTCACCCC TTCCAACACT CCATCCAGAG ATGAAGACGC AAGATCTCGC
    CGTATTTCAC GCTCTCGCTC ACCCTCCACC GCCAGTGACA TGGAGCCTAT AGAGTTGATT
    GACCACACAT CACGTAGTCT TCAGACCCCA ACAGGCAGAT CAGGCTACGG GAGCTTGGGA
    CACTCATCAC CTAAGCCCAA GGCACACCAG TTTAGTGTAA AGTCCTTCAA TGTTCCCACT
    AAATGTAACC AGTGCACCTC TCTCATGGTG GGACTCATCC GTCAGGGCTG CACATGCGAA
    GCCTGCAGCT TCTCCTGTCA TGTCACTTGT GCTGATAAAG CCCCTTCTGT GTGCCCCGTC
    GCACCAGACC AGACCAGGGG CAAACTGGGC ATTGACACTC AGAGGGGCAT TGGGACTGCA
    TATGAGGGGC ATCTTAGGGT GCCGAAGCCC ACTGGGGTGA AGAAGGGTTG GCAGAGGGTC
    TGGGCAGTGG TTTGTGACTT CAAACTTTTT CTGTATGAGC TTGGAGAAGG GAAAGGTGCT
    CAGCCTGGTG TTGTAGTAAA CCAGGTCATT GACATGAGGG ATGAAGAGTT TTCCGCCAGT
    TCGGTTCTGG AGTCTGATGT CATCCATGCT AATAGGAAGG ATATACCCTG TATTTTTAGG
    GTAACAGCAT CTCAGCTGTC CGCCTCCTCC AGTCAGAAGA CGTGTATTCT AATTCTGGCT
    GACAACGAGC AAGAAAGGAC GAAGTGGGTG TGCGCTCTGA ATGAACTGCA TCGCATCCTG
    AGGAAAAACA AGCTGAAGGA GCGTTTCGTC TATGTGCCTA AAGAAGCCTA CGACAGCACA
    CTTCCTCTCA TCAAAACCAC CCAAACTGCT GCTATTATAG ATCATGAACG CGTTGCTTTG
    GGCAATGAGG AGGGCCTGTT TGTCATCCAC GTCACCAAAG ATGAAATCAT TCGTGTGGGT
    GATGTCAAGA AGGTGCATCA TGTAGAACTG ATGCCCACGG AGCACATACT GGCCGTCATC
    TCGGGCAGGA ACCGACAAGT ATGTCTGGTC TCCATGGCGG GTCTCGACGG GAGGGAGGTC
    GACAAGAATA AGGTGGCAGA TACTAAAAAC TGCCAGTTGC TGGTGTCCGG TGTGGTTCGC
    AATGTCACCT GCTTCTGCCT CGCCATTAAA CGGCAGATCT CATGCTTCGA AATTAACAAG
    AGCAAGTCAC GACACTCCCA TCTCGTGGAC ATACAAGCTC CAGGAACTGT CCAATGGATG
    GCGCTGTTCA ACCAGCAGTT GTGTGTTGGT TACCCATCAG GCTTCATGCG CTACAGTTTG
    TATGGAGAAA CACCTCCAGC CAGCTTACTT CATCCAGATG ACCCCACACT GGCATTTATT
    AAAACAGACA ACCTGGATGC CCTTTGTGCT GTGGAAATCT CCAATAAGGA ACTGCTGCTC
    TGCTTCAGTA AAATAGCAGT GTATGTTGAC ACACACGGCA GACGCTCGCG CCAGCAGGAG
    CTGATGTGGC CCGCGGCGCC CACCGCCTGC TGCTATAACG CCCCCTATCT GTCGGTCTAC
    AGTGAAAATG CAGTGGATGT GTTTGATGTG AACTCTACGG AATGGATTCA GACCATTCCT
    CTCAAAAAGG TCCGGCCATT AAACATGGAT GGCTCTTTGA ATCTCCTGGG CCTAGAAACG
    GTCAGACTCA TCTACTTCAG AAACAAGACC GCAGAGGGGG ATGAACTGGT GGTGCCTGAA
    GTGTGTGATA ACAGCAGAAA GCAGATGGTG CGAAGCATGA GCAACAAGAG ACGATTCTCG
    TTTCGTATTC CTGAGGAGGA GAGACTGCAG CAGAGGAGAG AAATGTTGAA AGATCCTGAG
    AGGAGGAACA AGCTGATCTC AAATCCTACA AATTTTAACC ATGTGGCTCA TATGGGACCT
    GGAGATGGCA TGCAGATACT CAGAGACCTG CCAATGAATG TACGTGCTCA GGACAACCGT
    GCTGGATTCA GCGGCTCCGT CAGCATCCCC TCCATTACAA AAAACAGAGC AGAACCAGGG
    CGATCCATGA GCGCAAGCAG TGGCCTAGGA ATGCGGTCGT CCTCTCAGAA CGGCAGTGCT
    CTAAGGAGGG AGCTGTCCAG CGGCAGCTAC AGCTCTAAAC GGCAGACCAT GACTTCACCC
    TCCGAGAGTT CGCTGTCATC AGGAGGAGGG ATGGACGTGG GGGAAGCGCC GCTCTCTCAG
    TTTGACAGAG AGGACTCTGA TTCGCCGCGG CACTCCACTG CCTCCAACAG CTCCACCTTC
    AGCAGCCCGC CGAGCCCCGC CTCCCCACAC AAGACAAAGT CCCTCTCATT GGAGAGCACA
    GACCGCACCG CCTGGGACAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207996.1
    CDS1650..6953
    Translation

    Target ORF information:

    RefSeq Version XM_017352507.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352507.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC
    TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG
    CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC
    CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT
    GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC
    TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA
    GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC
    GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA
    GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA
    CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT
    GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG
    ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC
    CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA
    GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG
    AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG
    AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC
    AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC
    AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT
    CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG
    CTCGAGGGCA TGAAGTTGAA GCAGACTGGG CCTCCTAACG GAGCTGCAAG CTCAGACCAG
    CAGCAGGAGC TGATGCGCGT TCGGGCTGAA CTGGAGAAGA AGAGCGTACA GTACGAGGAG
    GAGATGTGTC GCAGAGAGAC GCTCCACAGC AGTGAGATGA AGAACCTCCA AAAAGAGCTA
    CGGGATGCCG AGGGTCAACA CCTCACACAT CAGAAAGAGA TACTCGTGCT TAAAGACAAA
    CTGGAGAAAA CTCGCAGAGA AAGTCAAGTC GAGCGAGAAG AATTTGTGTG CGAGTACAAG
    CAGAAGTACG AGAGGGAAAA AGCCCATCTG ACAGACGAAA ACAAGAAGCT GAGTGCCGAA
    GTGGAGAAGT TGAACGGTCA GTTCGAGCAG CTGAGCAGCA GTCACCGGCA GCTGGAGGAG
    GAGATGAGAG AACTGGCTGA TAAGAAGGAG AGTGTTGCCC ACTGGGAGGC CCAGATCACC
    GAGATCATCC AGTGGGTGAG CGATGAGAAG GATGCCCGTG GTTACCTTCA AGCTCTGGCC
    ACCAAAATGA CAGAGGAGCT GGAAGGGCTC AGAAACACCA GTTTGGGAGC CAGAGGAACG
    GACATGCCGT GGAAGATGAG GCGTTTCGCC AAGCTGGACA TGTCTGCGCG TCTGGAGCTG
    CAGTCGGCGC TGGATACTGA GATCAGAGCC AAACAGTCCA TCCAAGACGA GCTTAACAAA
    GTCAAAGCAA CCAACATTGC CACAGAATGC AAATTGCAAG AATCCGAAAG TAGAAACCAG
    GAGCTGCTGG GAGAAATTGA GAGACTGAGG AAGGAGACAG AGGAGCTAAG GCTGAGGAGA
    GGCGTGAAGC ACCAGGATTC CCAAAATTCC TTTTTGGCTT TCCTCAATGC GCCCACTTCT
    GCACTGGATC AGTTTGATCG CTCACCCTCT TTTGGTCCCG GAAGCAAAAC CAGACGTCTT
    GACTCTGCAG ATTTCACCCC TTCCAACACT CCATCCAGAG ATGAAGACGC AAGATCTCGC
    CGTATTTCAC GCTCTCGCTC ACCCTCCACC GCCAGTGACA TGGAGCCTAT AGAGTTGATT
    GACCACACAT CACGTAGTCT TCAGACCCCA ACAGGCAGAT CAGGCTACGG GAGCTTGGGA
    CACTCATCAC CTAAGCCCAA GGCACACCAG TTTAGTGTAA AGTCCTTCAA TGTTCCCACT
    AAATGTAACC AGTGCACCTC TCTCATGGTG GGACTCATCC GTCAGGGCTG CACATGCGAA
    GCCTGCAGCT TCTCCTGTCA TGTCACTTGT GCTGATAAAG CCCCTTCTGT GTGCCCCGTC
    GCACCAGACC AGACCAGGGG CAAACTGGGC ATTGACACTC AGAGGGGCAT TGGGACTGCA
    TATGAGGGGC ATCTTAGGGT GCCGAAGCCC ACTGGGGTGA AGAAGGGTTG GCAGAGGGTC
    TGGGCAGTGG TTTGTGACTT CAAACTTTTT CTGTATGAGC TTGGAGAAGG GAAAGGTGCT
    CAGCCTGGTG TTGTAGTAAA CCAGGTCATT GACATGAGGG ATGAAGAGTT TTCCGCCAGT
    TCGGTTCTGG AGTCTGATGT CATCCATGCT AATAGGAAGG ATATACCCTG TATTTTTAGG
    GTAACAGCAT CTCAGCTGTC CGCCTCCTCC AGTCAGAAGA CGTGTATTCT AATTCTGGCT
    GACAACGAGC AAGAAAGGAC GAAGTGGGTG TGCGCTCTGA ATGAACTGCA TCGCATCCTG
    AGGAAAAACA AGCTGAAGGA GCGTTTCGTC TATGTGCCTA AAGAAGCCTA CGACAGCACA
    CTTCCTCTCA TCAAAACCAC CCAAACTGCT GCTATTATAG ATCATGAACG CGTTGCTTTG
    GGCAATGAGG AGGGCCTGTT TGTCATCCAC GTCACCAAAG ATGAAATCAT TCGTGTGGGT
    GATGTCAAGA AGGTGCATCA TGTAGAACTG ATGCCCACGG AGCACATACT GGCCGTCATC
    TCGGGCAGGA ACCGACAAGT ATGTCTGGTC TCCATGGCGG GTCTCGACGG GAGGGAGGTC
    GACAAGAATA AGGTGGCAGA TACTAAAAAC TGCCAGTTGC TGGTGTCCGG TGTGGTTCGC
    AATGTCACCT GCTTCTGCCT CGCCATTAAA CGGCAGATCT CATGCTTCGA AATTAACAAG
    AGCAAGTCAC GACACTCCCA TCTCGTGGAC ATACAAGCTC CAGGAACTGT CCAATGGATG
    GCGCTGTTCA ACCAGCAGTT GTGTGTTGGT TACCCATCAG GCTTCATGCG CTACAGTTTG
    TATGGAGAAA CACCTCCAGC CAGCTTACTT CATCCAGATG ACCCCACACT GGCATTTATT
    AAAACAGACA ACCTGGATGC CCTTTGTGCT GTGGAAATCT CCAATAAGGA ACTGCTGCTC
    TGCTTCAGTA AAATAGCAGT GTATGTTGAC ACACACGGCA GACGCTCGCG CCAGCAGGAG
    CTGATGTGGC CCGCGGCGCC CACCGCCTGC TGCTATAACG CCCCCTATCT GTCGGTCTAC
    AGTGAAAATG CAGTGGATGT GTTTGATGTG AACTCTACGG AATGGATTCA GACCATTCCT
    CTCAAAAAGG TCCGGCCATT AAACATGGAT GGCTCTTTGA ATCTCCTGGG CCTAGAAACG
    GTCAGACTCA TCTACTTCAG AAACAAGACC GCAGAGGGGG ATGAACTGGT GGTGCCTGAA
    GTGTGTGATA ACAGCAGAAA GCAGATGGTG CGAAGCATGA GCAACAAGAG ACGATTCTCG
    TTTCGTATTC CTGAGGAGGA GAGACTGCAG CAGAGGAGAG AAATGTTGAA AGATCCTGAG
    AGGAGGAACA AGCTGATCTC AAATCCTACA AATTTTAACC ATGTGGCTCA TATGGGACCT
    GGAGATGGCA TGCAGATACT CAGAGACCTG CCAATGAATG TACGTGCTCA GGACAACCGT
    GCTGGATTCA GCGGCTCCGT CAGCATCCCC TCCATTACAA AAAACAGAGC AGAACCAGGG
    CGATCCATGA GCGCAAGCAG TGGCCTAGGA ATGCGGTCGT CCTCTCAGAA CGGCAGTGCT
    CTAAGGAGGG AGCTGTCCAG CGGCAGCTAC AGCTCTAAAC GGCAGACCAT GACTTCACCC
    TCCGAGAGTT CGCTGTCATC AGGAGGAGGG ATGGACGTGG GGGAAGCGCC GCTCTCTCAG
    TTTGACAGAG AGGACTCTGA TTCGCCGCGG CACTCCACTG CCTCCAACAG CTCCACCTTC
    AGCAGCCCGC CGAGCCCCGC CTCCCCACAC AAGACAAAGT CCCTCTCATT GGAGAGCACA
    GACCGCACCG CCTGGGACAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56578
    Clone ID Related Accession (Same CDS sequence) XM_017352507.1 , XM_017352507.2
    Accession Version XM_017352507.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5304bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase MRCK alpha isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352507.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC
    TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG
    CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC
    CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT
    GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC
    TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA
    GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC
    GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA
    GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA
    CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT
    GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG
    ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC
    CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA
    GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG
    AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG
    AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC
    AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC
    AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT
    CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG
    CTCGAGGGCA TGAAGTTGAA GCAGACTGGG CCTCCTAACG GAGCTGCAAG CTCAGACCAG
    CAGCAGGAGC TGATGCGCGT TCGGGCTGAA CTGGAGAAGA AGAGCGTACA GTACGAGGAG
    GAGATGTGTC GCAGAGAGAC GCTCCACAGC AGTGAGATGA AGAACCTCCA AAAAGAGCTA
    CGGGATGCCG AGGGTCAACA CCTCACACAT CAGAAAGAGA TACTCGTGCT TAAAGACAAA
    CTGGAGAAAA CTCGCAGAGA AAGTCAAGTC GAGCGAGAAG AATTTGTGTG CGAGTACAAG
    CAGAAGTACG AGAGGGAAAA AGCCCATCTG ACAGACGAAA ACAAGAAGCT GAGTGCCGAA
    GTGGAGAAGT TGAACGGTCA GTTCGAGCAG CTGAGCAGCA GTCACCGGCA GCTGGAGGAG
    GAGATGAGAG AACTGGCTGA TAAGAAGGAG AGTGTTGCCC ACTGGGAGGC CCAGATCACC
    GAGATCATCC AGTGGGTGAG CGATGAGAAG GATGCCCGTG GTTACCTTCA AGCTCTGGCC
    ACCAAAATGA CAGAGGAGCT GGAAGGGCTC AGAAACACCA GTTTGGGAGC CAGAGGAACG
    GACATGCCGT GGAAGATGAG GCGTTTCGCC AAGCTGGACA TGTCTGCGCG TCTGGAGCTG
    CAGTCGGCGC TGGATACTGA GATCAGAGCC AAACAGTCCA TCCAAGACGA GCTTAACAAA
    GTCAAAGCAA CCAACATTGC CACAGAATGC AAATTGCAAG AATCCGAAAG TAGAAACCAG
    GAGCTGCTGG GAGAAATTGA GAGACTGAGG AAGGAGACAG AGGAGCTAAG GCTGAGGAGA
    GGCGTGAAGC ACCAGGATTC CCAAAATTCC TTTTTGGCTT TCCTCAATGC GCCCACTTCT
    GCACTGGATC AGTTTGATCG CTCACCCTCT TTTGGTCCCG GAAGCAAAAC CAGACGTCTT
    GACTCTGCAG ATTTCACCCC TTCCAACACT CCATCCAGAG ATGAAGACGC AAGATCTCGC
    CGTATTTCAC GCTCTCGCTC ACCCTCCACC GCCAGTGACA TGGAGCCTAT AGAGTTGATT
    GACCACACAT CACGTAGTCT TCAGACCCCA ACAGGCAGAT CAGGCTACGG GAGCTTGGGA
    CACTCATCAC CTAAGCCCAA GGCACACCAG TTTAGTGTAA AGTCCTTCAA TGTTCCCACT
    AAATGTAACC AGTGCACCTC TCTCATGGTG GGACTCATCC GTCAGGGCTG CACATGCGAA
    GCCTGCAGCT TCTCCTGTCA TGTCACTTGT GCTGATAAAG CCCCTTCTGT GTGCCCCGTC
    GCACCAGACC AGACCAGGGG CAAACTGGGC ATTGACACTC AGAGGGGCAT TGGGACTGCA
    TATGAGGGGC ATCTTAGGGT GCCGAAGCCC ACTGGGGTGA AGAAGGGTTG GCAGAGGGTC
    TGGGCAGTGG TTTGTGACTT CAAACTTTTT CTGTATGAGC TTGGAGAAGG GAAAGGTGCT
    CAGCCTGGTG TTGTAGTAAA CCAGGTCATT GACATGAGGG ATGAAGAGTT TTCCGCCAGT
    TCGGTTCTGG AGTCTGATGT CATCCATGCT AATAGGAAGG ATATACCCTG TATTTTTAGG
    GTAACAGCAT CTCAGCTGTC CGCCTCCTCC AGTCAGAAGA CGTGTATTCT AATTCTGGCT
    GACAACGAGC AAGAAAGGAC GAAGTGGGTG TGCGCTCTGA ATGAACTGCA TCGCATCCTG
    AGGAAAAACA AGCTGAAGGA GCGTTTCGTC TATGTGCCTA AAGAAGCCTA CGACAGCACA
    CTTCCTCTCA TCAAAACCAC CCAAACTGCT GCTATTATAG ATCATGAACG CGTTGCTTTG
    GGCAATGAGG AGGGCCTGTT TGTCATCCAC GTCACCAAAG ATGAAATCAT TCGTGTGGGT
    GATGTCAAGA AGGTGCATCA TGTAGAACTG ATGCCCACGG AGCACATACT GGCCGTCATC
    TCGGGCAGGA ACCGACAAGT ATGTCTGGTC TCCATGGCGG GTCTCGACGG GAGGGAGGTC
    GACAAGAATA AGGTGGCAGA TACTAAAAAC TGCCAGTTGC TGGTGTCCGG TGTGGTTCGC
    AATGTCACCT GCTTCTGCCT CGCCATTAAA CGGCAGATCT CATGCTTCGA AATTAACAAG
    AGCAAGTCAC GACACTCCCA TCTCGTGGAC ATACAAGCTC CAGGAACTGT CCAATGGATG
    GCGCTGTTCA ACCAGCAGTT GTGTGTTGGT TACCCATCAG GCTTCATGCG CTACAGTTTG
    TATGGAGAAA CACCTCCAGC CAGCTTACTT CATCCAGATG ACCCCACACT GGCATTTATT
    AAAACAGACA ACCTGGATGC CCTTTGTGCT GTGGAAATCT CCAATAAGGA ACTGCTGCTC
    TGCTTCAGTA AAATAGCAGT GTATGTTGAC ACACACGGCA GACGCTCGCG CCAGCAGGAG
    CTGATGTGGC CCGCGGCGCC CACCGCCTGC TGCTATAACG CCCCCTATCT GTCGGTCTAC
    AGTGAAAATG CAGTGGATGT GTTTGATGTG AACTCTACGG AATGGATTCA GACCATTCCT
    CTCAAAAAGG TCCGGCCATT AAACATGGAT GGCTCTTTGA ATCTCCTGGG CCTAGAAACG
    GTCAGACTCA TCTACTTCAG AAACAAGACC GCAGAGGGGG ATGAACTGGT GGTGCCTGAA
    GTGTGTGATA ACAGCAGAAA GCAGATGGTG CGAAGCATGA GCAACAAGAG ACGATTCTCG
    TTTCGTATTC CTGAGGAGGA GAGACTGCAG CAGAGGAGAG AAATGTTGAA AGATCCTGAG
    AGGAGGAACA AGCTGATCTC AAATCCTACA AATTTTAACC ATGTGGCTCA TATGGGACCT
    GGAGATGGCA TGCAGATACT CAGAGACCTG CCAATGAATG TACGTGCTCA GGACAACCGT
    GCTGGATTCA GCGGCTCCGT CAGCATCCCC TCCATTACAA AAAACAGAGC AGAACCAGGG
    CGATCCATGA GCGCAAGCAG TGGCCTAGGA ATGCGGTCGT CCTCTCAGAA CGGCAGTGCT
    CTAAGGAGGG AGCTGTCCAG CGGCAGCTAC AGCTCTAAAC GGCAGACCAT GACTTCACCC
    TCCGAGAGTT CGCTGTCATC AGGAGGAGGG ATGGACGTGG GGGAAGCGCC GCTCTCTCAG
    TTTGACAGAG AGGACTCTGA TTCGCCGCGG CACTCCACTG CCTCCAACAG CTCCACCTTC
    AGCAGCCCGC CGAGCCCCGC CTCCCCACAC AAGACAAAGT CCCTCTCATT GGAGAGCACA
    GACCGCACCG CCTGGGACAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207996.1
    CDS1641..6944
    Translation

    Target ORF information:

    RefSeq Version XM_017352507.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352507.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC
    TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG
    CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC
    CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT
    GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC
    TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA
    GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC
    GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA
    GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA
    CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT
    GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG
    ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC
    CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA
    GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG
    AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG
    AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC
    AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC
    AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT
    CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG
    CTCGAGGGCA TGAAGTTGAA GCAGACTGGG CCTCCTAACG GAGCTGCAAG CTCAGACCAG
    CAGCAGGAGC TGATGCGCGT TCGGGCTGAA CTGGAGAAGA AGAGCGTACA GTACGAGGAG
    GAGATGTGTC GCAGAGAGAC GCTCCACAGC AGTGAGATGA AGAACCTCCA AAAAGAGCTA
    CGGGATGCCG AGGGTCAACA CCTCACACAT CAGAAAGAGA TACTCGTGCT TAAAGACAAA
    CTGGAGAAAA CTCGCAGAGA AAGTCAAGTC GAGCGAGAAG AATTTGTGTG CGAGTACAAG
    CAGAAGTACG AGAGGGAAAA AGCCCATCTG ACAGACGAAA ACAAGAAGCT GAGTGCCGAA
    GTGGAGAAGT TGAACGGTCA GTTCGAGCAG CTGAGCAGCA GTCACCGGCA GCTGGAGGAG
    GAGATGAGAG AACTGGCTGA TAAGAAGGAG AGTGTTGCCC ACTGGGAGGC CCAGATCACC
    GAGATCATCC AGTGGGTGAG CGATGAGAAG GATGCCCGTG GTTACCTTCA AGCTCTGGCC
    ACCAAAATGA CAGAGGAGCT GGAAGGGCTC AGAAACACCA GTTTGGGAGC CAGAGGAACG
    GACATGCCGT GGAAGATGAG GCGTTTCGCC AAGCTGGACA TGTCTGCGCG TCTGGAGCTG
    CAGTCGGCGC TGGATACTGA GATCAGAGCC AAACAGTCCA TCCAAGACGA GCTTAACAAA
    GTCAAAGCAA CCAACATTGC CACAGAATGC AAATTGCAAG AATCCGAAAG TAGAAACCAG
    GAGCTGCTGG GAGAAATTGA GAGACTGAGG AAGGAGACAG AGGAGCTAAG GCTGAGGAGA
    GGCGTGAAGC ACCAGGATTC CCAAAATTCC TTTTTGGCTT TCCTCAATGC GCCCACTTCT
    GCACTGGATC AGTTTGATCG CTCACCCTCT TTTGGTCCCG GAAGCAAAAC CAGACGTCTT
    GACTCTGCAG ATTTCACCCC TTCCAACACT CCATCCAGAG ATGAAGACGC AAGATCTCGC
    CGTATTTCAC GCTCTCGCTC ACCCTCCACC GCCAGTGACA TGGAGCCTAT AGAGTTGATT
    GACCACACAT CACGTAGTCT TCAGACCCCA ACAGGCAGAT CAGGCTACGG GAGCTTGGGA
    CACTCATCAC CTAAGCCCAA GGCACACCAG TTTAGTGTAA AGTCCTTCAA TGTTCCCACT
    AAATGTAACC AGTGCACCTC TCTCATGGTG GGACTCATCC GTCAGGGCTG CACATGCGAA
    GCCTGCAGCT TCTCCTGTCA TGTCACTTGT GCTGATAAAG CCCCTTCTGT GTGCCCCGTC
    GCACCAGACC AGACCAGGGG CAAACTGGGC ATTGACACTC AGAGGGGCAT TGGGACTGCA
    TATGAGGGGC ATCTTAGGGT GCCGAAGCCC ACTGGGGTGA AGAAGGGTTG GCAGAGGGTC
    TGGGCAGTGG TTTGTGACTT CAAACTTTTT CTGTATGAGC TTGGAGAAGG GAAAGGTGCT
    CAGCCTGGTG TTGTAGTAAA CCAGGTCATT GACATGAGGG ATGAAGAGTT TTCCGCCAGT
    TCGGTTCTGG AGTCTGATGT CATCCATGCT AATAGGAAGG ATATACCCTG TATTTTTAGG
    GTAACAGCAT CTCAGCTGTC CGCCTCCTCC AGTCAGAAGA CGTGTATTCT AATTCTGGCT
    GACAACGAGC AAGAAAGGAC GAAGTGGGTG TGCGCTCTGA ATGAACTGCA TCGCATCCTG
    AGGAAAAACA AGCTGAAGGA GCGTTTCGTC TATGTGCCTA AAGAAGCCTA CGACAGCACA
    CTTCCTCTCA TCAAAACCAC CCAAACTGCT GCTATTATAG ATCATGAACG CGTTGCTTTG
    GGCAATGAGG AGGGCCTGTT TGTCATCCAC GTCACCAAAG ATGAAATCAT TCGTGTGGGT
    GATGTCAAGA AGGTGCATCA TGTAGAACTG ATGCCCACGG AGCACATACT GGCCGTCATC
    TCGGGCAGGA ACCGACAAGT ATGTCTGGTC TCCATGGCGG GTCTCGACGG GAGGGAGGTC
    GACAAGAATA AGGTGGCAGA TACTAAAAAC TGCCAGTTGC TGGTGTCCGG TGTGGTTCGC
    AATGTCACCT GCTTCTGCCT CGCCATTAAA CGGCAGATCT CATGCTTCGA AATTAACAAG
    AGCAAGTCAC GACACTCCCA TCTCGTGGAC ATACAAGCTC CAGGAACTGT CCAATGGATG
    GCGCTGTTCA ACCAGCAGTT GTGTGTTGGT TACCCATCAG GCTTCATGCG CTACAGTTTG
    TATGGAGAAA CACCTCCAGC CAGCTTACTT CATCCAGATG ACCCCACACT GGCATTTATT
    AAAACAGACA ACCTGGATGC CCTTTGTGCT GTGGAAATCT CCAATAAGGA ACTGCTGCTC
    TGCTTCAGTA AAATAGCAGT GTATGTTGAC ACACACGGCA GACGCTCGCG CCAGCAGGAG
    CTGATGTGGC CCGCGGCGCC CACCGCCTGC TGCTATAACG CCCCCTATCT GTCGGTCTAC
    AGTGAAAATG CAGTGGATGT GTTTGATGTG AACTCTACGG AATGGATTCA GACCATTCCT
    CTCAAAAAGG TCCGGCCATT AAACATGGAT GGCTCTTTGA ATCTCCTGGG CCTAGAAACG
    GTCAGACTCA TCTACTTCAG AAACAAGACC GCAGAGGGGG ATGAACTGGT GGTGCCTGAA
    GTGTGTGATA ACAGCAGAAA GCAGATGGTG CGAAGCATGA GCAACAAGAG ACGATTCTCG
    TTTCGTATTC CTGAGGAGGA GAGACTGCAG CAGAGGAGAG AAATGTTGAA AGATCCTGAG
    AGGAGGAACA AGCTGATCTC AAATCCTACA AATTTTAACC ATGTGGCTCA TATGGGACCT
    GGAGATGGCA TGCAGATACT CAGAGACCTG CCAATGAATG TACGTGCTCA GGACAACCGT
    GCTGGATTCA GCGGCTCCGT CAGCATCCCC TCCATTACAA AAAACAGAGC AGAACCAGGG
    CGATCCATGA GCGCAAGCAG TGGCCTAGGA ATGCGGTCGT CCTCTCAGAA CGGCAGTGCT
    CTAAGGAGGG AGCTGTCCAG CGGCAGCTAC AGCTCTAAAC GGCAGACCAT GACTTCACCC
    TCCGAGAGTT CGCTGTCATC AGGAGGAGGG ATGGACGTGG GGGAAGCGCC GCTCTCTCAG
    TTTGACAGAG AGGACTCTGA TTCGCCGCGG CACTCCACTG CCTCCAACAG CTCCACCTTC
    AGCAGCCCGC CGAGCCCCGC CTCCCCACAC AAGACAAAGT CCCTCTCATT GGAGAGCACA
    GACCGCACCG CCTGGGACAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa33549
    Clone ID Related Accession (Same CDS sequence) NM_001045094.1
    Accession Version NM_001045094.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5151bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase MRCK alpha
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX470246.11 and BX511007.16. On or before Jul 22, 2013 this sequence version replaced NM_001113343.1, XM_689268.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168447, SAMEA4476738 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC
    TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG
    CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC
    CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT
    GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC
    TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA
    GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC
    GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA
    GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA
    CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT
    GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG
    ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC
    CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA
    GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG
    AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG
    AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC
    AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC
    AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT
    CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG
    CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC
    CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG
    GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG
    CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC
    AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC
    AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC
    GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG
    GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC
    ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG
    GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA
    ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG
    CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC
    AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC
    CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG
    AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT
    TCTGCACTGG ATCAGTTTGA TTTGATTGAC CACACATCAC GTAGTCTTCA GACCCCAACA
    GGCAGATCAG GCTACGGGAG CTTGGGACAC TCATCACCTA AGCCCAAGGC ACACCAGTTT
    AGTGTAAAGT CCTTCAATGT TCCCACTAAA TGTAACCAGT GCACCTCTCT CATGGTGGGA
    CTCATCCGTC AGGGCTGCAC ATGCGAAGCC TGCAGCTTCT CCTGTCATGT CACTTGTGCT
    GATAAAGCCC CTTCTGTGTG CCCCGTCGCA CCAGACCAGA CCAGGGGCAA ACTGGGCATT
    GACACTCAGA GGGGCATTGG GACTGCATAT GAGGGGCATC TTAGGGTGCC GAAGCCCACT
    GGGGTGAAGA AGGGTTGGCA GAGGGTCTGG GCAGTGGTTT GTGACTTCAA ACTTTTTCTG
    TATGAGCTTG GAGAAGGGAA AGGTGCTCAG CCTGGTGTTG TAGTAAACCA GGTCATTGAC
    ATGAGGGATG AAGAGTTTTC CGCCAGTTCG GTTCTGGAGT CTGATGTCAT CCATGCTAAT
    AGGAAGGATA TACCCTGTAT TTTTAGGGTA ACAGCATCTC AGCTGTCCGC CTCCTCCAGT
    CAGAAGACGT GTATTCTAAT TCTGGCTGAC AACGAGCAAG AAAGGACGAA GTGGGTGTGC
    GCTCTGAATG AACTGCATCG CATCCTGAGG AAAAACAAGC TGAAGGAGCG TTTCGTCTAT
    GTGCCTAAAG AAGCCTACGA CAGCACACTT CCTCTCATCA AAACCACCCA AACTGCTGCT
    ATTATAGATC ATGAACGCGT TGCTTTGGGC AATGAGGAGG GCCTGTTTGT CATCCACGTC
    ACCAAAGATG AAATCATTCG TGTGGGTGAT GTCAAGAAGG TGCATCATGT AGAACTGATG
    CCCACGGAGC ACATACTGGC CGTCATCTCG GGCAGGAACC GACAAGTATG TCTGGTCTCC
    ATGGCGGGTC TCGACGGGAG GGAGGTCGAC AAGAATAAGG TGGCAGATAC TAAAAACTGC
    CAGTTGCTGG TGTCCGGTGT GGTTCGCAAT GTCACCTGCT TCTGCCTCGC CATTAAACGG
    CAGATCTCAT GCTTCGAAAT TAACAAGAGC AAGTCACGAC ACTCCCATCT CGTGGACATA
    CAAGCTCCAG GAACTGTCCA ATGGATGGCG CTGTTCAACC AGCAGTTGTG TGTTGGTTAC
    CCATCAGGCT TCATGCGCTA CAGTTTGTAT GGAGAAACAC CTCCAGCCAG CTTACTTCAT
    CCAGATGACC CCACACTGGC ATTTATTAAA ACAGACAACC TGGATGCCCT TTGTGCTGTG
    GAAATCTCCA ATAAGGAACT GCTGCTCTGC TTCAGTAAAA TAGCAGTGTA TGTTGACACA
    CACGGCAGAC GCTCGCGCCA GCAGGAGCTG ATGTGGCCCG CGGCGCCCAC CGCCTGCTGC
    TATAACGCCC CCTATCTGTC GGTCTACAGT GAAAATGCAG TGGATGTGTT TGATGTGAAC
    TCTACGGAAT GGATTCAGAC CATTCCTCTC AAAAAGGTCC GGCCATTAAA CATGGATGGC
    TCTTTGAATC TCCTGGGCCT AGAAACGGTC AGACTCATCT ACTTCAGAAA CAAGACCGCA
    GAGGGGGATG AACTGGTGGT GCCTGAAGTG TGTGATAACA GCAGAAAGCA GATGGTGCGA
    AGCATGAGCA ACAAGAGACG ATTCTCGTTT CGTATTCCTG AGGAGGAGAG ACTGCAGCAG
    AGGAGAGAAA TGTTGAAAGA TCCTGAGAGG AGGAACAAGC TGATCTCAAA TCCTACAAAT
    TTTAACCATG TGGCTCATAT GGGACCTGGA GATGGCATGC AGATACTCAG AGACCTGCCA
    ATGAATGTAC GTGCTCAGGA CAACCGTGCT GGATTCAGCG GCTCCGTCAG CATCCCCTCC
    ATTACAAAAA ACAGAGCAGA ACCAGGGCGA TCCATGAGCG CAAGCAGTGG CCTAGGAATG
    CGGTCGTCCT CTCAGAACGG CAGTGCTCTA AGGAGGGAGC TGTCCAGCGG CAGCTACAGC
    TCTAAACGGC AGACCATGAC TTCACCCTCC GAGAGTTCGC TGTCATCAGG AGGAGGGATG
    GACGTGGGGG AAGCGCCGCT CTCTCAGTTT GACAGAGAGG ACTCTGATTC GCCGCGGCAC
    TCCACTGCCT CCAACAGCTC CACCTTCAGC AGCCCGCCGA GCCCCGCCTC CCCACACAAG
    ACAAAGTCCC TCTCATTGGA GAGCACAGAC CGCACCGCCT GGGACACATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001038559.1
    CDS1..5151
    Translation

    Target ORF information:

    RefSeq Version NM_001045094.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio CDC42 binding protein kinase alpha (DMPK-like) b (cdc42bpab), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001045094.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC
    TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG
    CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC
    CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT
    GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC
    TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA
    GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC
    GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA
    GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA
    CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT
    GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG
    ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC
    CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA
    GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG
    AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG
    AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC
    AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC
    AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT
    CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG
    CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC
    CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG
    GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG
    CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC
    AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC
    AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC
    GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG
    GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC
    ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG
    GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA
    ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG
    CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC
    AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC
    CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG
    AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT
    TCTGCACTGG ATCAGTTTGA TTTGATTGAC CACACATCAC GTAGTCTTCA GACCCCAACA
    GGCAGATCAG GCTACGGGAG CTTGGGACAC TCATCACCTA AGCCCAAGGC ACACCAGTTT
    AGTGTAAAGT CCTTCAATGT TCCCACTAAA TGTAACCAGT GCACCTCTCT CATGGTGGGA
    CTCATCCGTC AGGGCTGCAC ATGCGAAGCC TGCAGCTTCT CCTGTCATGT CACTTGTGCT
    GATAAAGCCC CTTCTGTGTG CCCCGTCGCA CCAGACCAGA CCAGGGGCAA ACTGGGCATT
    GACACTCAGA GGGGCATTGG GACTGCATAT GAGGGGCATC TTAGGGTGCC GAAGCCCACT
    GGGGTGAAGA AGGGTTGGCA GAGGGTCTGG GCAGTGGTTT GTGACTTCAA ACTTTTTCTG
    TATGAGCTTG GAGAAGGGAA AGGTGCTCAG CCTGGTGTTG TAGTAAACCA GGTCATTGAC
    ATGAGGGATG AAGAGTTTTC CGCCAGTTCG GTTCTGGAGT CTGATGTCAT CCATGCTAAT
    AGGAAGGATA TACCCTGTAT TTTTAGGGTA ACAGCATCTC AGCTGTCCGC CTCCTCCAGT
    CAGAAGACGT GTATTCTAAT TCTGGCTGAC AACGAGCAAG AAAGGACGAA GTGGGTGTGC
    GCTCTGAATG AACTGCATCG CATCCTGAGG AAAAACAAGC TGAAGGAGCG TTTCGTCTAT
    GTGCCTAAAG AAGCCTACGA CAGCACACTT CCTCTCATCA AAACCACCCA AACTGCTGCT
    ATTATAGATC ATGAACGCGT TGCTTTGGGC AATGAGGAGG GCCTGTTTGT CATCCACGTC
    ACCAAAGATG AAATCATTCG TGTGGGTGAT GTCAAGAAGG TGCATCATGT AGAACTGATG
    CCCACGGAGC ACATACTGGC CGTCATCTCG GGCAGGAACC GACAAGTATG TCTGGTCTCC
    ATGGCGGGTC TCGACGGGAG GGAGGTCGAC AAGAATAAGG TGGCAGATAC TAAAAACTGC
    CAGTTGCTGG TGTCCGGTGT GGTTCGCAAT GTCACCTGCT TCTGCCTCGC CATTAAACGG
    CAGATCTCAT GCTTCGAAAT TAACAAGAGC AAGTCACGAC ACTCCCATCT CGTGGACATA
    CAAGCTCCAG GAACTGTCCA ATGGATGGCG CTGTTCAACC AGCAGTTGTG TGTTGGTTAC
    CCATCAGGCT TCATGCGCTA CAGTTTGTAT GGAGAAACAC CTCCAGCCAG CTTACTTCAT
    CCAGATGACC CCACACTGGC ATTTATTAAA ACAGACAACC TGGATGCCCT TTGTGCTGTG
    GAAATCTCCA ATAAGGAACT GCTGCTCTGC TTCAGTAAAA TAGCAGTGTA TGTTGACACA
    CACGGCAGAC GCTCGCGCCA GCAGGAGCTG ATGTGGCCCG CGGCGCCCAC CGCCTGCTGC
    TATAACGCCC CCTATCTGTC GGTCTACAGT GAAAATGCAG TGGATGTGTT TGATGTGAAC
    TCTACGGAAT GGATTCAGAC CATTCCTCTC AAAAAGGTCC GGCCATTAAA CATGGATGGC
    TCTTTGAATC TCCTGGGCCT AGAAACGGTC AGACTCATCT ACTTCAGAAA CAAGACCGCA
    GAGGGGGATG AACTGGTGGT GCCTGAAGTG TGTGATAACA GCAGAAAGCA GATGGTGCGA
    AGCATGAGCA ACAAGAGACG ATTCTCGTTT CGTATTCCTG AGGAGGAGAG ACTGCAGCAG
    AGGAGAGAAA TGTTGAAAGA TCCTGAGAGG AGGAACAAGC TGATCTCAAA TCCTACAAAT
    TTTAACCATG TGGCTCATAT GGGACCTGGA GATGGCATGC AGATACTCAG AGACCTGCCA
    ATGAATGTAC GTGCTCAGGA CAACCGTGCT GGATTCAGCG GCTCCGTCAG CATCCCCTCC
    ATTACAAAAA ACAGAGCAGA ACCAGGGCGA TCCATGAGCG CAAGCAGTGG CCTAGGAATG
    CGGTCGTCCT CTCAGAACGG CAGTGCTCTA AGGAGGGAGC TGTCCAGCGG CAGCTACAGC
    TCTAAACGGC AGACCATGAC TTCACCCTCC GAGAGTTCGC TGTCATCAGG AGGAGGGATG
    GACGTGGGGG AAGCGCCGCT CTCTCAGTTT GACAGAGAGG ACTCTGATTC GCCGCGGCAC
    TCCACTGCCT CCAACAGCTC CACCTTCAGC AGCCCGCCGA GCCCCGCCTC CCCACACAAG
    ACAAAGTCCC TCTCATTGGA GAGCACAGAC CGCACCGCCT GGGACACATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa25024
    Clone ID Related Accession (Same CDS sequence) XM_005160749.4 , XM_005160749.3
    Accession Version XM_005160749.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5382bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase MRCK alpha isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160749.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC
    TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG
    CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC
    CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT
    GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC
    TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA
    GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC
    GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA
    GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA
    CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT
    GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG
    ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC
    CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA
    GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG
    AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG
    AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC
    AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC
    AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT
    CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG
    CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC
    CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG
    GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG
    CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC
    AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC
    AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC
    GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG
    GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC
    ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG
    GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA
    ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG
    CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC
    AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC
    CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG
    AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT
    TCTGCACTGG ATCAGTTTGA TCGCTCACCC TCTTTTGGTC CCGGAAGCAA AACCAGACGT
    CTTGACTCTG CAGATTTCAC CCCTTCCAAC ACTCCATCCA GAGATGAAGA CGCAAGATCT
    CGCCGTATTT CACGCTCTCG CTCACCCTCC ACCGCCAGTG ACATGGAGCC TATAGAGTTG
    ATTGACCACA CATCACGTAG TCTTCAGACC CCAACAGGCA GATCAGGCTA CGGGAGCTTG
    GGACACTCAT CACCTAAGCC CAAGGCACAC CAGTTTAGTG TAAAGTCCTT CAATGTTCCC
    ACTAAATGTA ACCAGTGCAC CTCTCTCATG GTGGGACTCA TCCGTCAGGG CTGCACATGC
    GAAGCCTGCA GCTTCTCCTG TCATGTCACT TGTGCTGATA AAGCCCCTTC TGTGTGCCCC
    GTCGCACCAG ACCAGACCAG GGGCAAACTG GGCATTGACA CTCAGAGGGG CATTGGGACT
    GCATATGAGG GGCATCTTAG GGTGCCGAAG CCCACTGGGG TGAAGAAGGG TTGGCAGAGG
    GTCTGGGCAG TGGTTTGTGA CTTCAAACTT TTTCTGTATG AGCTTGGAGA AGGGAAAGGT
    GCTCAGCCTG GTGTTGTAGT AAACCAGGTC ATTGACATGA GGGATGAAGA GTTTTCCGCC
    AGTTCGGTTC TGGAGTCTGA TGTCATCCAT GCTAATAGGA AGGATATACC CTGTATTTTT
    AGGGTAACAG CATCTCAGCT GTCCGCCTCC TCCAGTCAGA AGACGTGTAT TCTAATTCTG
    GCTGACAACG AGCAAGAAAG GACGAAGTGG GTGTGCGCTC TGAATGAACT GCATCGCATC
    CTGAGGAAAA ACAAGCTGAA GGAGCGTTTC GTCTATGTGC CTAAAGAAGC CTACGACAGC
    ACACTTCCTC TCATCAAAAC CACCCAAACT GCTGCTATTA TAGATCATGA ACGCGTTGCT
    TTGGGCAATG AGGAGGGCCT GTTTGTCATC CACGTCACCA AAGATGAAAT CATTCGTGTG
    GGTGATGTCA AGAAGGTGCA TCATGTAGAA CTGATGCCCA CGGAGCACAT ACTGGCCGTC
    ATCTCGGGCA GGAACCGACA AGTATGTCTG GTCTCCATGG CGGGTCTCGA CGGGAGGGAG
    GTCGACAAGA ATAAGGTGGC AGATACTAAA AACTGCCAGT TGCTGGTGTC CGGTGTGGTT
    CGCAATGTCA CCTGCTTCTG CCTCGCCATT AAACGGCAGA TCTCATGCTT CGAAATTAAC
    AAGAGCAAGT CACGACACTC CCATCTCGTG GACATACAAG CTCCAGGAAC TGTCCAATGG
    ATGGCGCTGT TCAACCAGCA GTTGTGTGTT GGTTACCCAT CAGGCTTCAT GCGCTACAGT
    TTGTATGGAG AAACACCTCC AGCCAGCTTA CTTCATCCAG ATGACCCCAC ACTGGCATTT
    ATTAAAACAG ACAACCTGGA TGCCCTTTGT GCTGTGGAAA TCTCCAATAA GGAACTGCTG
    CTCTGCTTCA GTAAAATAGC AGTGTATGTT GACACACACG GCAGACGCTC GCGCCAGCAG
    GAGCTGATGT GGCCCGCGGC GCCCACCGCC TGCTGCTATA ACGCCCCCTA TCTGTCGGTC
    TACAGTGAAA ATGCAGTGGA TGTGTTTGAT GTGAACTCTA CGGAATGGAT TCAGACCATT
    CCTCTCAAAA AGGTCCGGCC ATTAAACATG GATGGCTCTT TGAATCTCCT GGGCCTAGAA
    ACGGTCAGAC TCATCTACTT CAGAAACAAG ACCGCAGAGG GGGATGAACT GGTGGTGCCT
    GAAGTGTGTG ATAACAGCAG AAAGCAGATG GTGCGAAGCA TGAGCAACAA GAGACGATTC
    TCGTTTCGTA TTCCTGAGGA GGAGAGACTG CAGCAGAGGA GAGAAATGTT GAAAGATCCT
    GAGAGGAGGA ACAAGCTGAT CTCAAATCCT ACAAATTTTA ACCATGTGGC TCATATGGGA
    CCTGGAGATG GCATGCAGAT ACTCAGAGAC CTGCCAATGA ATGTACGTGC TCAGGACAAC
    CGTGCTGGAT TCAGCGGCTC CGTCAGCATC CCCTCCATTA CAAAAAACAG AGCAGAACCA
    GGGCGATCCA TGAGCGCAAG CAGTGGCCTA GGAATGCGGT CGTCCTCTCA GAACGGCAGT
    GCTCTAAGGA GGGAGCTGTC CAGCGGCAGC TACAGCTCTA AACGGCAGAC CATGACTTCA
    CCCTCCGAGA GTTCGCTGTC ATCAGGAGGA GGGATGGACG TGGGGGAAGC GCCGCTCTCT
    CAGTTTGACA GAGAGTGGCA GGCAGTATCG CCCTCGGAGA AGACCCCTGA TCTGAAAAGG
    GCTTTAAGGA CCCTGCTTAG TAAGATGAAG GACTCTGATT CGCCGCGGCA CTCCACTGCC
    TCCAACAGCT CCACCTTCAG CAGCCCGCCG AGCCCCGCCT CCCCACACAA GACAAAGTCC
    CTCTCATTGG AGAGCACAGA CCGCACCGCC TGGGACACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160806.1
    CDS1641..7022
    Translation

    Target ORF information:

    RefSeq Version XM_005160749.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005160749.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC
    TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG
    CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC
    CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT
    GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC
    TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA
    GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC
    GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA
    GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA
    CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT
    GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG
    ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC
    CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA
    GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG
    AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG
    AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC
    AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC
    AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT
    CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG
    CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC
    CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG
    GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG
    CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC
    AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC
    AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC
    GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG
    GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC
    ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG
    GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA
    ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG
    CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC
    AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC
    CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG
    AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT
    TCTGCACTGG ATCAGTTTGA TCGCTCACCC TCTTTTGGTC CCGGAAGCAA AACCAGACGT
    CTTGACTCTG CAGATTTCAC CCCTTCCAAC ACTCCATCCA GAGATGAAGA CGCAAGATCT
    CGCCGTATTT CACGCTCTCG CTCACCCTCC ACCGCCAGTG ACATGGAGCC TATAGAGTTG
    ATTGACCACA CATCACGTAG TCTTCAGACC CCAACAGGCA GATCAGGCTA CGGGAGCTTG
    GGACACTCAT CACCTAAGCC CAAGGCACAC CAGTTTAGTG TAAAGTCCTT CAATGTTCCC
    ACTAAATGTA ACCAGTGCAC CTCTCTCATG GTGGGACTCA TCCGTCAGGG CTGCACATGC
    GAAGCCTGCA GCTTCTCCTG TCATGTCACT TGTGCTGATA AAGCCCCTTC TGTGTGCCCC
    GTCGCACCAG ACCAGACCAG GGGCAAACTG GGCATTGACA CTCAGAGGGG CATTGGGACT
    GCATATGAGG GGCATCTTAG GGTGCCGAAG CCCACTGGGG TGAAGAAGGG TTGGCAGAGG
    GTCTGGGCAG TGGTTTGTGA CTTCAAACTT TTTCTGTATG AGCTTGGAGA AGGGAAAGGT
    GCTCAGCCTG GTGTTGTAGT AAACCAGGTC ATTGACATGA GGGATGAAGA GTTTTCCGCC
    AGTTCGGTTC TGGAGTCTGA TGTCATCCAT GCTAATAGGA AGGATATACC CTGTATTTTT
    AGGGTAACAG CATCTCAGCT GTCCGCCTCC TCCAGTCAGA AGACGTGTAT TCTAATTCTG
    GCTGACAACG AGCAAGAAAG GACGAAGTGG GTGTGCGCTC TGAATGAACT GCATCGCATC
    CTGAGGAAAA ACAAGCTGAA GGAGCGTTTC GTCTATGTGC CTAAAGAAGC CTACGACAGC
    ACACTTCCTC TCATCAAAAC CACCCAAACT GCTGCTATTA TAGATCATGA ACGCGTTGCT
    TTGGGCAATG AGGAGGGCCT GTTTGTCATC CACGTCACCA AAGATGAAAT CATTCGTGTG
    GGTGATGTCA AGAAGGTGCA TCATGTAGAA CTGATGCCCA CGGAGCACAT ACTGGCCGTC
    ATCTCGGGCA GGAACCGACA AGTATGTCTG GTCTCCATGG CGGGTCTCGA CGGGAGGGAG
    GTCGACAAGA ATAAGGTGGC AGATACTAAA AACTGCCAGT TGCTGGTGTC CGGTGTGGTT
    CGCAATGTCA CCTGCTTCTG CCTCGCCATT AAACGGCAGA TCTCATGCTT CGAAATTAAC
    AAGAGCAAGT CACGACACTC CCATCTCGTG GACATACAAG CTCCAGGAAC TGTCCAATGG
    ATGGCGCTGT TCAACCAGCA GTTGTGTGTT GGTTACCCAT CAGGCTTCAT GCGCTACAGT
    TTGTATGGAG AAACACCTCC AGCCAGCTTA CTTCATCCAG ATGACCCCAC ACTGGCATTT
    ATTAAAACAG ACAACCTGGA TGCCCTTTGT GCTGTGGAAA TCTCCAATAA GGAACTGCTG
    CTCTGCTTCA GTAAAATAGC AGTGTATGTT GACACACACG GCAGACGCTC GCGCCAGCAG
    GAGCTGATGT GGCCCGCGGC GCCCACCGCC TGCTGCTATA ACGCCCCCTA TCTGTCGGTC
    TACAGTGAAA ATGCAGTGGA TGTGTTTGAT GTGAACTCTA CGGAATGGAT TCAGACCATT
    CCTCTCAAAA AGGTCCGGCC ATTAAACATG GATGGCTCTT TGAATCTCCT GGGCCTAGAA
    ACGGTCAGAC TCATCTACTT CAGAAACAAG ACCGCAGAGG GGGATGAACT GGTGGTGCCT
    GAAGTGTGTG ATAACAGCAG AAAGCAGATG GTGCGAAGCA TGAGCAACAA GAGACGATTC
    TCGTTTCGTA TTCCTGAGGA GGAGAGACTG CAGCAGAGGA GAGAAATGTT GAAAGATCCT
    GAGAGGAGGA ACAAGCTGAT CTCAAATCCT ACAAATTTTA ACCATGTGGC TCATATGGGA
    CCTGGAGATG GCATGCAGAT ACTCAGAGAC CTGCCAATGA ATGTACGTGC TCAGGACAAC
    CGTGCTGGAT TCAGCGGCTC CGTCAGCATC CCCTCCATTA CAAAAAACAG AGCAGAACCA
    GGGCGATCCA TGAGCGCAAG CAGTGGCCTA GGAATGCGGT CGTCCTCTCA GAACGGCAGT
    GCTCTAAGGA GGGAGCTGTC CAGCGGCAGC TACAGCTCTA AACGGCAGAC CATGACTTCA
    CCCTCCGAGA GTTCGCTGTC ATCAGGAGGA GGGATGGACG TGGGGGAAGC GCCGCTCTCT
    CAGTTTGACA GAGAGTGGCA GGCAGTATCG CCCTCGGAGA AGACCCCTGA TCTGAAAAGG
    GCTTTAAGGA CCCTGCTTAG TAAGATGAAG GACTCTGATT CGCCGCGGCA CTCCACTGCC
    TCCAACAGCT CCACCTTCAG CAGCCCGCCG AGCCCCGCCT CCCCACACAA GACAAAGTCC
    CTCTCATTGG AGAGCACAGA CCGCACCGCC TGGGACACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa25024
    Clone ID Related Accession (Same CDS sequence) XM_005160749.4 , XM_005160749.3
    Accession Version XM_005160749.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5382bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product serine/threonine-protein kinase MRCK alpha isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160749.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC
    TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG
    CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC
    CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT
    GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC
    TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA
    GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC
    GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA
    GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA
    CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT
    GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG
    ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC
    CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA
    GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG
    AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG
    AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC
    AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC
    AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT
    CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG
    CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC
    CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG
    GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG
    CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC
    AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC
    AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC
    GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG
    GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC
    ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG
    GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA
    ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG
    CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC
    AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC
    CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG
    AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT
    TCTGCACTGG ATCAGTTTGA TCGCTCACCC TCTTTTGGTC CCGGAAGCAA AACCAGACGT
    CTTGACTCTG CAGATTTCAC CCCTTCCAAC ACTCCATCCA GAGATGAAGA CGCAAGATCT
    CGCCGTATTT CACGCTCTCG CTCACCCTCC ACCGCCAGTG ACATGGAGCC TATAGAGTTG
    ATTGACCACA CATCACGTAG TCTTCAGACC CCAACAGGCA GATCAGGCTA CGGGAGCTTG
    GGACACTCAT CACCTAAGCC CAAGGCACAC CAGTTTAGTG TAAAGTCCTT CAATGTTCCC
    ACTAAATGTA ACCAGTGCAC CTCTCTCATG GTGGGACTCA TCCGTCAGGG CTGCACATGC
    GAAGCCTGCA GCTTCTCCTG TCATGTCACT TGTGCTGATA AAGCCCCTTC TGTGTGCCCC
    GTCGCACCAG ACCAGACCAG GGGCAAACTG GGCATTGACA CTCAGAGGGG CATTGGGACT
    GCATATGAGG GGCATCTTAG GGTGCCGAAG CCCACTGGGG TGAAGAAGGG TTGGCAGAGG
    GTCTGGGCAG TGGTTTGTGA CTTCAAACTT TTTCTGTATG AGCTTGGAGA AGGGAAAGGT
    GCTCAGCCTG GTGTTGTAGT AAACCAGGTC ATTGACATGA GGGATGAAGA GTTTTCCGCC
    AGTTCGGTTC TGGAGTCTGA TGTCATCCAT GCTAATAGGA AGGATATACC CTGTATTTTT
    AGGGTAACAG CATCTCAGCT GTCCGCCTCC TCCAGTCAGA AGACGTGTAT TCTAATTCTG
    GCTGACAACG AGCAAGAAAG GACGAAGTGG GTGTGCGCTC TGAATGAACT GCATCGCATC
    CTGAGGAAAA ACAAGCTGAA GGAGCGTTTC GTCTATGTGC CTAAAGAAGC CTACGACAGC
    ACACTTCCTC TCATCAAAAC CACCCAAACT GCTGCTATTA TAGATCATGA ACGCGTTGCT
    TTGGGCAATG AGGAGGGCCT GTTTGTCATC CACGTCACCA AAGATGAAAT CATTCGTGTG
    GGTGATGTCA AGAAGGTGCA TCATGTAGAA CTGATGCCCA CGGAGCACAT ACTGGCCGTC
    ATCTCGGGCA GGAACCGACA AGTATGTCTG GTCTCCATGG CGGGTCTCGA CGGGAGGGAG
    GTCGACAAGA ATAAGGTGGC AGATACTAAA AACTGCCAGT TGCTGGTGTC CGGTGTGGTT
    CGCAATGTCA CCTGCTTCTG CCTCGCCATT AAACGGCAGA TCTCATGCTT CGAAATTAAC
    AAGAGCAAGT CACGACACTC CCATCTCGTG GACATACAAG CTCCAGGAAC TGTCCAATGG
    ATGGCGCTGT TCAACCAGCA GTTGTGTGTT GGTTACCCAT CAGGCTTCAT GCGCTACAGT
    TTGTATGGAG AAACACCTCC AGCCAGCTTA CTTCATCCAG ATGACCCCAC ACTGGCATTT
    ATTAAAACAG ACAACCTGGA TGCCCTTTGT GCTGTGGAAA TCTCCAATAA GGAACTGCTG
    CTCTGCTTCA GTAAAATAGC AGTGTATGTT GACACACACG GCAGACGCTC GCGCCAGCAG
    GAGCTGATGT GGCCCGCGGC GCCCACCGCC TGCTGCTATA ACGCCCCCTA TCTGTCGGTC
    TACAGTGAAA ATGCAGTGGA TGTGTTTGAT GTGAACTCTA CGGAATGGAT TCAGACCATT
    CCTCTCAAAA AGGTCCGGCC ATTAAACATG GATGGCTCTT TGAATCTCCT GGGCCTAGAA
    ACGGTCAGAC TCATCTACTT CAGAAACAAG ACCGCAGAGG GGGATGAACT GGTGGTGCCT
    GAAGTGTGTG ATAACAGCAG AAAGCAGATG GTGCGAAGCA TGAGCAACAA GAGACGATTC
    TCGTTTCGTA TTCCTGAGGA GGAGAGACTG CAGCAGAGGA GAGAAATGTT GAAAGATCCT
    GAGAGGAGGA ACAAGCTGAT CTCAAATCCT ACAAATTTTA ACCATGTGGC TCATATGGGA
    CCTGGAGATG GCATGCAGAT ACTCAGAGAC CTGCCAATGA ATGTACGTGC TCAGGACAAC
    CGTGCTGGAT TCAGCGGCTC CGTCAGCATC CCCTCCATTA CAAAAAACAG AGCAGAACCA
    GGGCGATCCA TGAGCGCAAG CAGTGGCCTA GGAATGCGGT CGTCCTCTCA GAACGGCAGT
    GCTCTAAGGA GGGAGCTGTC CAGCGGCAGC TACAGCTCTA AACGGCAGAC CATGACTTCA
    CCCTCCGAGA GTTCGCTGTC ATCAGGAGGA GGGATGGACG TGGGGGAAGC GCCGCTCTCT
    CAGTTTGACA GAGAGTGGCA GGCAGTATCG CCCTCGGAGA AGACCCCTGA TCTGAAAAGG
    GCTTTAAGGA CCCTGCTTAG TAAGATGAAG GACTCTGATT CGCCGCGGCA CTCCACTGCC
    TCCAACAGCT CCACCTTCAG CAGCCCGCCG AGCCCCGCCT CCCCACACAA GACAAAGTCC
    CTCTCATTGG AGAGCACAGA CCGCACCGCC TGGGACACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160806.1
    CDS1650..7031
    Translation

    Target ORF information:

    RefSeq Version XM_005160749.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005160749.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC
    TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG
    CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC
    CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT
    GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC
    TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA
    GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC
    GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA
    GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA
    CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT
    GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG
    ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC
    CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA
    GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG
    AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG
    AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC
    AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC
    AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT
    CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG
    CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC
    CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG
    GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG
    CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC
    AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC
    AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC
    GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG
    GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC
    ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG
    GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA
    ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG
    CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC
    AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC
    CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG
    AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT
    TCTGCACTGG ATCAGTTTGA TCGCTCACCC TCTTTTGGTC CCGGAAGCAA AACCAGACGT
    CTTGACTCTG CAGATTTCAC CCCTTCCAAC ACTCCATCCA GAGATGAAGA CGCAAGATCT
    CGCCGTATTT CACGCTCTCG CTCACCCTCC ACCGCCAGTG ACATGGAGCC TATAGAGTTG
    ATTGACCACA CATCACGTAG TCTTCAGACC CCAACAGGCA GATCAGGCTA CGGGAGCTTG
    GGACACTCAT CACCTAAGCC CAAGGCACAC CAGTTTAGTG TAAAGTCCTT CAATGTTCCC
    ACTAAATGTA ACCAGTGCAC CTCTCTCATG GTGGGACTCA TCCGTCAGGG CTGCACATGC
    GAAGCCTGCA GCTTCTCCTG TCATGTCACT TGTGCTGATA AAGCCCCTTC TGTGTGCCCC
    GTCGCACCAG ACCAGACCAG GGGCAAACTG GGCATTGACA CTCAGAGGGG CATTGGGACT
    GCATATGAGG GGCATCTTAG GGTGCCGAAG CCCACTGGGG TGAAGAAGGG TTGGCAGAGG
    GTCTGGGCAG TGGTTTGTGA CTTCAAACTT TTTCTGTATG AGCTTGGAGA AGGGAAAGGT
    GCTCAGCCTG GTGTTGTAGT AAACCAGGTC ATTGACATGA GGGATGAAGA GTTTTCCGCC
    AGTTCGGTTC TGGAGTCTGA TGTCATCCAT GCTAATAGGA AGGATATACC CTGTATTTTT
    AGGGTAACAG CATCTCAGCT GTCCGCCTCC TCCAGTCAGA AGACGTGTAT TCTAATTCTG
    GCTGACAACG AGCAAGAAAG GACGAAGTGG GTGTGCGCTC TGAATGAACT GCATCGCATC
    CTGAGGAAAA ACAAGCTGAA GGAGCGTTTC GTCTATGTGC CTAAAGAAGC CTACGACAGC
    ACACTTCCTC TCATCAAAAC CACCCAAACT GCTGCTATTA TAGATCATGA ACGCGTTGCT
    TTGGGCAATG AGGAGGGCCT GTTTGTCATC CACGTCACCA AAGATGAAAT CATTCGTGTG
    GGTGATGTCA AGAAGGTGCA TCATGTAGAA CTGATGCCCA CGGAGCACAT ACTGGCCGTC
    ATCTCGGGCA GGAACCGACA AGTATGTCTG GTCTCCATGG CGGGTCTCGA CGGGAGGGAG
    GTCGACAAGA ATAAGGTGGC AGATACTAAA AACTGCCAGT TGCTGGTGTC CGGTGTGGTT
    CGCAATGTCA CCTGCTTCTG CCTCGCCATT AAACGGCAGA TCTCATGCTT CGAAATTAAC
    AAGAGCAAGT CACGACACTC CCATCTCGTG GACATACAAG CTCCAGGAAC TGTCCAATGG
    ATGGCGCTGT TCAACCAGCA GTTGTGTGTT GGTTACCCAT CAGGCTTCAT GCGCTACAGT
    TTGTATGGAG AAACACCTCC AGCCAGCTTA CTTCATCCAG ATGACCCCAC ACTGGCATTT
    ATTAAAACAG ACAACCTGGA TGCCCTTTGT GCTGTGGAAA TCTCCAATAA GGAACTGCTG
    CTCTGCTTCA GTAAAATAGC AGTGTATGTT GACACACACG GCAGACGCTC GCGCCAGCAG
    GAGCTGATGT GGCCCGCGGC GCCCACCGCC TGCTGCTATA ACGCCCCCTA TCTGTCGGTC
    TACAGTGAAA ATGCAGTGGA TGTGTTTGAT GTGAACTCTA CGGAATGGAT TCAGACCATT
    CCTCTCAAAA AGGTCCGGCC ATTAAACATG GATGGCTCTT TGAATCTCCT GGGCCTAGAA
    ACGGTCAGAC TCATCTACTT CAGAAACAAG ACCGCAGAGG GGGATGAACT GGTGGTGCCT
    GAAGTGTGTG ATAACAGCAG AAAGCAGATG GTGCGAAGCA TGAGCAACAA GAGACGATTC
    TCGTTTCGTA TTCCTGAGGA GGAGAGACTG CAGCAGAGGA GAGAAATGTT GAAAGATCCT
    GAGAGGAGGA ACAAGCTGAT CTCAAATCCT ACAAATTTTA ACCATGTGGC TCATATGGGA
    CCTGGAGATG GCATGCAGAT ACTCAGAGAC CTGCCAATGA ATGTACGTGC TCAGGACAAC
    CGTGCTGGAT TCAGCGGCTC CGTCAGCATC CCCTCCATTA CAAAAAACAG AGCAGAACCA
    GGGCGATCCA TGAGCGCAAG CAGTGGCCTA GGAATGCGGT CGTCCTCTCA GAACGGCAGT
    GCTCTAAGGA GGGAGCTGTC CAGCGGCAGC TACAGCTCTA AACGGCAGAC CATGACTTCA
    CCCTCCGAGA GTTCGCTGTC ATCAGGAGGA GGGATGGACG TGGGGGAAGC GCCGCTCTCT
    CAGTTTGACA GAGAGTGGCA GGCAGTATCG CCCTCGGAGA AGACCCCTGA TCTGAAAAGG
    GCTTTAAGGA CCCTGCTTAG TAAGATGAAG GACTCTGATT CGCCGCGGCA CTCCACTGCC
    TCCAACAGCT CCACCTTCAG CAGCCCGCCG AGCCCCGCCT CCCCACACAA GACAAAGTCC
    CTCTCATTGG AGAGCACAGA CCGCACCGCC TGGGACACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa25025
    Clone ID Related Accession (Same CDS sequence) XM_005160750.3
    Accession Version XM_005160750.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5367bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product serine/threonine-protein kinase MRCK alpha isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160750.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC
    TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG
    CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC
    CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT
    GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC
    TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA
    GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC
    GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA
    GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA
    CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT
    GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG
    ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC
    CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA
    GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG
    AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG
    AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC
    AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC
    AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT
    CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG
    CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC
    CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG
    GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG
    CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC
    AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC
    AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC
    GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG
    GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC
    ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG
    GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA
    ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG
    CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC
    AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC
    CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG
    AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT
    TCTGCACTGG ATCAGTTTGA TCGCTCACCC TCTTTTGGTC CCGGAAGCAA AACCAGACGT
    CTTGACTCTG CAGATTTCAC CCCTTCCAAC ACTCCATCCA GAGATGAAGA CGCAAGATCT
    CGCCGTATTT CACGCTCTCG CTCACCCTCC ACCGCCAGTG ACATGGAGCC TATAGAGTTG
    ATTGACCACA CATCACGTAG TCTTCAGACC CCAACAGGCA GATCAGGCTA CGGGAGCTTG
    GGACACTCAT CACCTAAGCC CAAGGCACAC CAGTTTAGTG TAAAGTCCTT CAATGTTCCC
    ACTAAATGTA ACCAGTGCAC CTCTCTCATG GTGGGACTCA TCCGTCAGGG CTGCACATGC
    GAAGCCTGCA GCTTCTCCTG TCATGTCACT TGTGCTGATA AAGCCCCTTC TGTGTGCCCC
    GTCGCACCAG ACCAGACCAG GGGCAAACTG GGCATTGACA CTCAGAGGGG CATTGGGACT
    GCATATGAGG GGCATCTTAG GGTGCCGAAG CCCACTGGGG TGAAGAAGGG TTGGCAGAGG
    GTCTGGGCAG TGGTTTGTGA CTTCAAACTT TTTCTGTATG AGCTTGGAGA AGGGAAAGGT
    GCTCAGCCTG GTGTTGTAGT AAACCAGGTC ATTGACATGA GGGATGAAGA GTTTTCCGCC
    AGTTCGGTTC TGGAGTCTGA TGTCATCCAT GCTAATAGGA AGGATATACC CTGTATTTTT
    AGGGTAACAG CATCTCAGCT GTCCGCCTCC TCCAGTCAGA AGACGTGTAT TCTAATTCTG
    GCTGACAACG AGCAAGAAAG GACGAAGTGG GTGTGCGCTC TGAATGAACT GCATCGCATC
    CTGAGGAAAA ACAAGCTGAA GGAGCGTTTC GTCTATGTGC CTAAAGAAGC CTACGACAGC
    ACACTTCCTC TCATCAAAAC CACCCAAACT GCTGCTATTA TAGATCATGA ACGCGTTGCT
    TTGGGCAATG AGGAGGGCCT GTTTGTCATC CACGTCACCA AAGATGAAAT CATTCGTGTG
    GGTGATGTCA AGAAGGTGCA TCATGTAGAA CTGATGCCCA CGGAGCACAT ACTGGCCGTC
    ATCTCGGGCA GGAACCGACA AGTATGTCTG GTCTCCATGG CGGGTCTCGA CGGGAGGGAG
    GTCGACAAGA ATAAGGTGGC AGATACTAAA AACTGCCAGT TGCTGGTGTC CGGTGTGGTT
    CGCAATGTCA CCTGCTTCTG CCTCGCCATT AAACGGCAGA TCTCATGCTT CGAAATTAAC
    AAGAGCAAGT CACGACACTC CCATCTCGTG GACATACAAG CTCCAGGAAC TGTCCAATGG
    ATGGCGCTGT TCAACCAGCA GTTGTGTGTT GGTTACCCAT CAGGCTTCAT GCGCTACAGT
    TTGTATGGAG AAACACCTCC AGCCAGCTTA CTTCATCCAG ATGACCCCAC ACTGGCATTT
    ATTAAAACAG ACAACCTGGA TGCCCTTTGT GCTGTGGAAA TCTCCAATAA GGAACTGCTG
    CTCTGCTTCA GTAAAATAGC AGTGTATGTT GACACACACG GCAGACGCTC GCGCCAGCAG
    GAGCTGATGT GGCCCGCGGC GCCCACCGCC TGCTGCTATA ACGCCCCCTA TCTGTCGGTC
    TACAGTGAAA ATGCAGTGGA TGTGTTTGAT GTGAACTCTA CGGAATGGAT TCAGACCATT
    CCTCTCAAAA AGGTCCGGCC ATTAAACATG GATGGCTCTT TGAATCTCCT GGGCCTAGAA
    ACGGTCAGAC TCATCTACTT CAGAAACAAG ACCGCAGAGG GGGATGAACT GGTGGTGCCT
    GAAGTGTGTG ATAACAGCAG AAAGCAGATG GTGCGAAGCA TGAGCAACAA GAGACGATTC
    TCGTTTCGTA TTCCTGAGGA GGAGAGACTG CAGCAGAGGA GAGAAATGTT GAAAGATCCT
    GAGAGGAGGA ACAAGCTGAT CTCAAATCCT ACAAATTTTA ACCATGTGGC TCATATGGGA
    CCTGGAGATG GCATGCAGAT ACTCAGAGAC CTGCCAATGG ACAACCGTGC TGGATTCAGC
    GGCTCCGTCA GCATCCCCTC CATTACAAAA AACAGAGCAG AACCAGGGCG ATCCATGAGC
    GCAAGCAGTG GCCTAGGAAT GCGGTCGTCC TCTCAGAACG GCAGTGCTCT AAGGAGGGAG
    CTGTCCAGCG GCAGCTACAG CTCTAAACGG CAGACCATGA CTTCACCCTC CGAGAGTTCG
    CTGTCATCAG GAGGAGGGAT GGACGTGGGG GAAGCGCCGC TCTCTCAGTT TGACAGAGAG
    TGGCAGGCAG TATCGCCCTC GGAGAAGACC CCTGATCTGA AAAGGGCTTT AAGGACCCTG
    CTTAGTAAGA TGAAGGACTC TGATTCGCCG CGGCACTCCA CTGCCTCCAA CAGCTCCACC
    TTCAGCAGCC CGCCGAGCCC CGCCTCCCCA CACAAGACAA AGTCCCTCTC ATTGGAGAGC
    ACAGACCGCA CCGCCTGGGA CACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160807.1
    CDS1650..7016
    Translation

    Target ORF information:

    RefSeq Version XM_005160750.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005160750.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC
    TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG
    CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC
    CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT
    GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC
    TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA
    GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC
    GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA
    GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA
    CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT
    GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG
    ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC
    CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA
    GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG
    AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG
    AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC
    AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC
    AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT
    CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG
    CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC
    CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG
    GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG
    CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC
    AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC
    AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC
    GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG
    GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC
    ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG
    GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA
    ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG
    CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC
    AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC
    CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG
    AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT
    TCTGCACTGG ATCAGTTTGA TCGCTCACCC TCTTTTGGTC CCGGAAGCAA AACCAGACGT
    CTTGACTCTG CAGATTTCAC CCCTTCCAAC ACTCCATCCA GAGATGAAGA CGCAAGATCT
    CGCCGTATTT CACGCTCTCG CTCACCCTCC ACCGCCAGTG ACATGGAGCC TATAGAGTTG
    ATTGACCACA CATCACGTAG TCTTCAGACC CCAACAGGCA GATCAGGCTA CGGGAGCTTG
    GGACACTCAT CACCTAAGCC CAAGGCACAC CAGTTTAGTG TAAAGTCCTT CAATGTTCCC
    ACTAAATGTA ACCAGTGCAC CTCTCTCATG GTGGGACTCA TCCGTCAGGG CTGCACATGC
    GAAGCCTGCA GCTTCTCCTG TCATGTCACT TGTGCTGATA AAGCCCCTTC TGTGTGCCCC
    GTCGCACCAG ACCAGACCAG GGGCAAACTG GGCATTGACA CTCAGAGGGG CATTGGGACT
    GCATATGAGG GGCATCTTAG GGTGCCGAAG CCCACTGGGG TGAAGAAGGG TTGGCAGAGG
    GTCTGGGCAG TGGTTTGTGA CTTCAAACTT TTTCTGTATG AGCTTGGAGA AGGGAAAGGT
    GCTCAGCCTG GTGTTGTAGT AAACCAGGTC ATTGACATGA GGGATGAAGA GTTTTCCGCC
    AGTTCGGTTC TGGAGTCTGA TGTCATCCAT GCTAATAGGA AGGATATACC CTGTATTTTT
    AGGGTAACAG CATCTCAGCT GTCCGCCTCC TCCAGTCAGA AGACGTGTAT TCTAATTCTG
    GCTGACAACG AGCAAGAAAG GACGAAGTGG GTGTGCGCTC TGAATGAACT GCATCGCATC
    CTGAGGAAAA ACAAGCTGAA GGAGCGTTTC GTCTATGTGC CTAAAGAAGC CTACGACAGC
    ACACTTCCTC TCATCAAAAC CACCCAAACT GCTGCTATTA TAGATCATGA ACGCGTTGCT
    TTGGGCAATG AGGAGGGCCT GTTTGTCATC CACGTCACCA AAGATGAAAT CATTCGTGTG
    GGTGATGTCA AGAAGGTGCA TCATGTAGAA CTGATGCCCA CGGAGCACAT ACTGGCCGTC
    ATCTCGGGCA GGAACCGACA AGTATGTCTG GTCTCCATGG CGGGTCTCGA CGGGAGGGAG
    GTCGACAAGA ATAAGGTGGC AGATACTAAA AACTGCCAGT TGCTGGTGTC CGGTGTGGTT
    CGCAATGTCA CCTGCTTCTG CCTCGCCATT AAACGGCAGA TCTCATGCTT CGAAATTAAC
    AAGAGCAAGT CACGACACTC CCATCTCGTG GACATACAAG CTCCAGGAAC TGTCCAATGG
    ATGGCGCTGT TCAACCAGCA GTTGTGTGTT GGTTACCCAT CAGGCTTCAT GCGCTACAGT
    TTGTATGGAG AAACACCTCC AGCCAGCTTA CTTCATCCAG ATGACCCCAC ACTGGCATTT
    ATTAAAACAG ACAACCTGGA TGCCCTTTGT GCTGTGGAAA TCTCCAATAA GGAACTGCTG
    CTCTGCTTCA GTAAAATAGC AGTGTATGTT GACACACACG GCAGACGCTC GCGCCAGCAG
    GAGCTGATGT GGCCCGCGGC GCCCACCGCC TGCTGCTATA ACGCCCCCTA TCTGTCGGTC
    TACAGTGAAA ATGCAGTGGA TGTGTTTGAT GTGAACTCTA CGGAATGGAT TCAGACCATT
    CCTCTCAAAA AGGTCCGGCC ATTAAACATG GATGGCTCTT TGAATCTCCT GGGCCTAGAA
    ACGGTCAGAC TCATCTACTT CAGAAACAAG ACCGCAGAGG GGGATGAACT GGTGGTGCCT
    GAAGTGTGTG ATAACAGCAG AAAGCAGATG GTGCGAAGCA TGAGCAACAA GAGACGATTC
    TCGTTTCGTA TTCCTGAGGA GGAGAGACTG CAGCAGAGGA GAGAAATGTT GAAAGATCCT
    GAGAGGAGGA ACAAGCTGAT CTCAAATCCT ACAAATTTTA ACCATGTGGC TCATATGGGA
    CCTGGAGATG GCATGCAGAT ACTCAGAGAC CTGCCAATGG ACAACCGTGC TGGATTCAGC
    GGCTCCGTCA GCATCCCCTC CATTACAAAA AACAGAGCAG AACCAGGGCG ATCCATGAGC
    GCAAGCAGTG GCCTAGGAAT GCGGTCGTCC TCTCAGAACG GCAGTGCTCT AAGGAGGGAG
    CTGTCCAGCG GCAGCTACAG CTCTAAACGG CAGACCATGA CTTCACCCTC CGAGAGTTCG
    CTGTCATCAG GAGGAGGGAT GGACGTGGGG GAAGCGCCGC TCTCTCAGTT TGACAGAGAG
    TGGCAGGCAG TATCGCCCTC GGAGAAGACC CCTGATCTGA AAAGGGCTTT AAGGACCCTG
    CTTAGTAAGA TGAAGGACTC TGATTCGCCG CGGCACTCCA CTGCCTCCAA CAGCTCCACC
    TTCAGCAGCC CGCCGAGCCC CGCCTCCCCA CACAAGACAA AGTCCCTCTC ATTGGAGAGC
    ACAGACCGCA CCGCCTGGGA CACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa25026
    Clone ID Related Accession (Same CDS sequence) XM_005160751.4 , XM_005160751.3
    Accession Version XM_005160751.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase MRCK alpha isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160751.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC
    TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG
    CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC
    CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT
    GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC
    TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA
    GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC
    GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA
    GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA
    CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT
    GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG
    ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC
    CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA
    GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG
    AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG
    AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC
    AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC
    AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT
    CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG
    CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC
    CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG
    GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG
    CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC
    AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC
    AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC
    GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG
    GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC
    ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG
    GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA
    ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG
    CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC
    AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC
    CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG
    AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT
    TCTGCACTGG ATCAGTTTGA TCGCTCACCC TCTTTTGGTC CCGGAAGCAA AACCAGACGT
    CTTGACTCTG CAGATTTCAC CCCTTCCAAC ACTCCATCCA GAGATGAAGA CGCAAGATCT
    CGCCGTATTT CACGCTCTCG CTCACCCTCC ACCGCCAGTG ACATGGAGCC TATAGAGTTG
    ATTGACCACA CATCACGTAG TCTTCAGACC CCAACAGGCA GATCAGGCTA CGGGAGCTTG
    GGACACTCAT CACCTAAGCC CAAGGCACAC CAGTTTAGTG TAAAGTCCTT CAATGTTCCC
    ACTAAATGTA ACCAGTGCAC CTCTCTCATG GTGGGACTCA TCCGTCAGGG CTGCACATGC
    GAAGCCTGCA GCTTCTCCTG TCATGTCACT TGTGCTGATA AAGCCCCTTC TGTGTGCCCC
    GTCGCACCAG ACCAGACCAG GGGCAAACTG GGCATTGACA CTCAGAGGGG CATTGGGACT
    GCATATGAGG GGCATCTTAG GGTGCCGAAG CCCACTGGGG TGAAGAAGGG TTGGCAGAGG
    GTCTGGGCAG TGGTTTGTGA CTTCAAACTT TTTCTGTATG AGCTTGGAGA AGGGAAAGGT
    GCTCAGCCTG GTGTTGTAGT AAACCAGGTC ATTGACATGA GGGATGAAGA GTTTTCCGCC
    AGTTCGGTTC TGGAGTCTGA TGTCATCCAT GCTAATAGGA AGGATATACC CTGTATTTTT
    AGGGTAACAG CATCTCAGCT GTCCGCCTCC TCCAGTCAGA AGACGTGTAT TCTAATTCTG
    GCTGACAACG AGCAAGAAAG GACGAAGTGG GTGTGCGCTC TGAATGAACT GCATCGCATC
    CTGAGGAAAA ACAAGCTGAA GGAGCGTTTC GTCTATGTGC CTAAAGAAGC CTACGACAGC
    ACACTTCCTC TCATCAAAAC CACCCAAACT GCTGCTATTA TAGATCATGA ACGCGTTGCT
    TTGGGCAATG AGGAGGGCCT GTTTGTCATC CACGTCACCA AAGATGAAAT CATTCGTGTG
    GGTGATGTCA AGAAGGTGCA TCATGTAGAA CTGATGCCCA CGGAGCACAT ACTGGCCGTC
    ATCTCGGGCA GGAACCGACA AGTATGTCTG GTCTCCATGG CGGGTCTCGA CGGGAGGGAG
    GTCGACAAGA ATAAGGTGGC AGATACTAAA AACTGCCAGT TGCTGGTGTC CGGTGTGGTT
    CGCAATGTCA CCTGCTTCTG CCTCGCCATT AAACGGCAGA TCTCATGCTT CGAAATTAAC
    AAGAGCAAGT CACGACACTC CCATCTCGTG GACATACAAG CTCCAGGAAC TGTCCAATGG
    ATGGCGCTGT TCAACCAGCA GTTGTGTGTT GGTTACCCAT CAGGCTTCAT GCGCTACAGT
    TTGTATGGAG AAACACCTCC AGCCAGCTTA CTTCATCCAG ATGACCCCAC ACTGGCATTT
    ATTAAAACAG ACAACCTGGA TGCCCTTTGT GCTGTGGAAA TCTCCAATAA GGAACTGCTG
    CTCTGCTTCA GTAAAATAGC AGTGTATGTT GACACACACG GCAGACGCTC GCGCCAGCAG
    GAGCTGATGT GGCCCGCGGC GCCCACCGCC TGCTGCTATA ACGCCCCCTA TCTGTCGGTC
    TACAGTGAAA ATGCAGTGGA TGTGTTTGAT GTGAACTCTA CGGAATGGAT TCAGACCATT
    CCTCTCAAAA AGGTCCGGCC ATTAAACATG GATGGCTCTT TGAATCTCCT GGGCCTAGAA
    ACGGTCAGAC TCATCTACTT CAGAAACAAG ACCGCAGAGG GGGATGAACT GGTGGTGCCT
    GAAGTGTGTG ATAACAGCAG AAAGCAGATG GTGCGAAGCA TGAGCAACAA GAGACGATTC
    TCGTTTCGTA TTCCTGAGGA GGAGAGACTG CAGCAGAGGA GAGAAATGTT GAAAGATCCT
    GAGAGGAGGA ACAAGCTGAT CTCAAATCCT ACAAATTTTA ACCATGTGGC TCATATGGGA
    CCTGGAGATG GCATGCAGAT ACTCAGAGAC CTGCCAATGA ATGTACGTGC TCAGGACAAC
    CGTGCTGGAT TCAGCGGCTC CGTCAGCATC CCCTCCATTA CAAAAAACAG AGCAGAACCA
    GGGCGATCCA TGAGCGCAAG CAGTGGCCTA GGAATGCGGT CGTCCTCTCA GAACGGCAGT
    GCTCTAAGGA GGGAGCTGTC CAGCGGCAGC TACAGCTCTA AACGGCAGAC CATGACTTCA
    CCCTCCGAGA GTTCGCTGTC ATCAGGAGGA GGGATGGACG TGGGGGAAGC GCCGCTCTCT
    CAGTTTGACA GAGAGGACTC TGATTCGCCG CGGCACTCCA CTGCCTCCAA CAGCTCCACC
    TTCAGCAGCC CGCCGAGCCC CGCCTCCCCA CACAAGACAA AGTCCCTCTC ATTGGAGAGC
    ACAGACCGCA CCGCCTGGGA CACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160808.1
    CDS1641..6947
    Translation

    Target ORF information:

    RefSeq Version XM_005160751.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005160751.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC
    TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG
    CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC
    CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT
    GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC
    TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA
    GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC
    GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA
    GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA
    CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT
    GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG
    ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC
    CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA
    GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG
    AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG
    AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC
    AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC
    AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT
    CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG
    CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC
    CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG
    GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG
    CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC
    AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC
    AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC
    GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG
    GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC
    ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG
    GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA
    ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG
    CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC
    AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC
    CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG
    AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT
    TCTGCACTGG ATCAGTTTGA TCGCTCACCC TCTTTTGGTC CCGGAAGCAA AACCAGACGT
    CTTGACTCTG CAGATTTCAC CCCTTCCAAC ACTCCATCCA GAGATGAAGA CGCAAGATCT
    CGCCGTATTT CACGCTCTCG CTCACCCTCC ACCGCCAGTG ACATGGAGCC TATAGAGTTG
    ATTGACCACA CATCACGTAG TCTTCAGACC CCAACAGGCA GATCAGGCTA CGGGAGCTTG
    GGACACTCAT CACCTAAGCC CAAGGCACAC CAGTTTAGTG TAAAGTCCTT CAATGTTCCC
    ACTAAATGTA ACCAGTGCAC CTCTCTCATG GTGGGACTCA TCCGTCAGGG CTGCACATGC
    GAAGCCTGCA GCTTCTCCTG TCATGTCACT TGTGCTGATA AAGCCCCTTC TGTGTGCCCC
    GTCGCACCAG ACCAGACCAG GGGCAAACTG GGCATTGACA CTCAGAGGGG CATTGGGACT
    GCATATGAGG GGCATCTTAG GGTGCCGAAG CCCACTGGGG TGAAGAAGGG TTGGCAGAGG
    GTCTGGGCAG TGGTTTGTGA CTTCAAACTT TTTCTGTATG AGCTTGGAGA AGGGAAAGGT
    GCTCAGCCTG GTGTTGTAGT AAACCAGGTC ATTGACATGA GGGATGAAGA GTTTTCCGCC
    AGTTCGGTTC TGGAGTCTGA TGTCATCCAT GCTAATAGGA AGGATATACC CTGTATTTTT
    AGGGTAACAG CATCTCAGCT GTCCGCCTCC TCCAGTCAGA AGACGTGTAT TCTAATTCTG
    GCTGACAACG AGCAAGAAAG GACGAAGTGG GTGTGCGCTC TGAATGAACT GCATCGCATC
    CTGAGGAAAA ACAAGCTGAA GGAGCGTTTC GTCTATGTGC CTAAAGAAGC CTACGACAGC
    ACACTTCCTC TCATCAAAAC CACCCAAACT GCTGCTATTA TAGATCATGA ACGCGTTGCT
    TTGGGCAATG AGGAGGGCCT GTTTGTCATC CACGTCACCA AAGATGAAAT CATTCGTGTG
    GGTGATGTCA AGAAGGTGCA TCATGTAGAA CTGATGCCCA CGGAGCACAT ACTGGCCGTC
    ATCTCGGGCA GGAACCGACA AGTATGTCTG GTCTCCATGG CGGGTCTCGA CGGGAGGGAG
    GTCGACAAGA ATAAGGTGGC AGATACTAAA AACTGCCAGT TGCTGGTGTC CGGTGTGGTT
    CGCAATGTCA CCTGCTTCTG CCTCGCCATT AAACGGCAGA TCTCATGCTT CGAAATTAAC
    AAGAGCAAGT CACGACACTC CCATCTCGTG GACATACAAG CTCCAGGAAC TGTCCAATGG
    ATGGCGCTGT TCAACCAGCA GTTGTGTGTT GGTTACCCAT CAGGCTTCAT GCGCTACAGT
    TTGTATGGAG AAACACCTCC AGCCAGCTTA CTTCATCCAG ATGACCCCAC ACTGGCATTT
    ATTAAAACAG ACAACCTGGA TGCCCTTTGT GCTGTGGAAA TCTCCAATAA GGAACTGCTG
    CTCTGCTTCA GTAAAATAGC AGTGTATGTT GACACACACG GCAGACGCTC GCGCCAGCAG
    GAGCTGATGT GGCCCGCGGC GCCCACCGCC TGCTGCTATA ACGCCCCCTA TCTGTCGGTC
    TACAGTGAAA ATGCAGTGGA TGTGTTTGAT GTGAACTCTA CGGAATGGAT TCAGACCATT
    CCTCTCAAAA AGGTCCGGCC ATTAAACATG GATGGCTCTT TGAATCTCCT GGGCCTAGAA
    ACGGTCAGAC TCATCTACTT CAGAAACAAG ACCGCAGAGG GGGATGAACT GGTGGTGCCT
    GAAGTGTGTG ATAACAGCAG AAAGCAGATG GTGCGAAGCA TGAGCAACAA GAGACGATTC
    TCGTTTCGTA TTCCTGAGGA GGAGAGACTG CAGCAGAGGA GAGAAATGTT GAAAGATCCT
    GAGAGGAGGA ACAAGCTGAT CTCAAATCCT ACAAATTTTA ACCATGTGGC TCATATGGGA
    CCTGGAGATG GCATGCAGAT ACTCAGAGAC CTGCCAATGA ATGTACGTGC TCAGGACAAC
    CGTGCTGGAT TCAGCGGCTC CGTCAGCATC CCCTCCATTA CAAAAAACAG AGCAGAACCA
    GGGCGATCCA TGAGCGCAAG CAGTGGCCTA GGAATGCGGT CGTCCTCTCA GAACGGCAGT
    GCTCTAAGGA GGGAGCTGTC CAGCGGCAGC TACAGCTCTA AACGGCAGAC CATGACTTCA
    CCCTCCGAGA GTTCGCTGTC ATCAGGAGGA GGGATGGACG TGGGGGAAGC GCCGCTCTCT
    CAGTTTGACA GAGAGGACTC TGATTCGCCG CGGCACTCCA CTGCCTCCAA CAGCTCCACC
    TTCAGCAGCC CGCCGAGCCC CGCCTCCCCA CACAAGACAA AGTCCCTCTC ATTGGAGAGC
    ACAGACCGCA CCGCCTGGGA CACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa25026
    Clone ID Related Accession (Same CDS sequence) XM_005160751.4 , XM_005160751.3
    Accession Version XM_005160751.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product serine/threonine-protein kinase MRCK alpha isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160751.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC
    TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG
    CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC
    CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT
    GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC
    TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA
    GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC
    GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA
    GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA
    CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT
    GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG
    ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC
    CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA
    GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG
    AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG
    AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC
    AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC
    AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT
    CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG
    CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC
    CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG
    GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG
    CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC
    AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC
    AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC
    GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG
    GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC
    ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG
    GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA
    ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG
    CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC
    AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC
    CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG
    AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT
    TCTGCACTGG ATCAGTTTGA TCGCTCACCC TCTTTTGGTC CCGGAAGCAA AACCAGACGT
    CTTGACTCTG CAGATTTCAC CCCTTCCAAC ACTCCATCCA GAGATGAAGA CGCAAGATCT
    CGCCGTATTT CACGCTCTCG CTCACCCTCC ACCGCCAGTG ACATGGAGCC TATAGAGTTG
    ATTGACCACA CATCACGTAG TCTTCAGACC CCAACAGGCA GATCAGGCTA CGGGAGCTTG
    GGACACTCAT CACCTAAGCC CAAGGCACAC CAGTTTAGTG TAAAGTCCTT CAATGTTCCC
    ACTAAATGTA ACCAGTGCAC CTCTCTCATG GTGGGACTCA TCCGTCAGGG CTGCACATGC
    GAAGCCTGCA GCTTCTCCTG TCATGTCACT TGTGCTGATA AAGCCCCTTC TGTGTGCCCC
    GTCGCACCAG ACCAGACCAG GGGCAAACTG GGCATTGACA CTCAGAGGGG CATTGGGACT
    GCATATGAGG GGCATCTTAG GGTGCCGAAG CCCACTGGGG TGAAGAAGGG TTGGCAGAGG
    GTCTGGGCAG TGGTTTGTGA CTTCAAACTT TTTCTGTATG AGCTTGGAGA AGGGAAAGGT
    GCTCAGCCTG GTGTTGTAGT AAACCAGGTC ATTGACATGA GGGATGAAGA GTTTTCCGCC
    AGTTCGGTTC TGGAGTCTGA TGTCATCCAT GCTAATAGGA AGGATATACC CTGTATTTTT
    AGGGTAACAG CATCTCAGCT GTCCGCCTCC TCCAGTCAGA AGACGTGTAT TCTAATTCTG
    GCTGACAACG AGCAAGAAAG GACGAAGTGG GTGTGCGCTC TGAATGAACT GCATCGCATC
    CTGAGGAAAA ACAAGCTGAA GGAGCGTTTC GTCTATGTGC CTAAAGAAGC CTACGACAGC
    ACACTTCCTC TCATCAAAAC CACCCAAACT GCTGCTATTA TAGATCATGA ACGCGTTGCT
    TTGGGCAATG AGGAGGGCCT GTTTGTCATC CACGTCACCA AAGATGAAAT CATTCGTGTG
    GGTGATGTCA AGAAGGTGCA TCATGTAGAA CTGATGCCCA CGGAGCACAT ACTGGCCGTC
    ATCTCGGGCA GGAACCGACA AGTATGTCTG GTCTCCATGG CGGGTCTCGA CGGGAGGGAG
    GTCGACAAGA ATAAGGTGGC AGATACTAAA AACTGCCAGT TGCTGGTGTC CGGTGTGGTT
    CGCAATGTCA CCTGCTTCTG CCTCGCCATT AAACGGCAGA TCTCATGCTT CGAAATTAAC
    AAGAGCAAGT CACGACACTC CCATCTCGTG GACATACAAG CTCCAGGAAC TGTCCAATGG
    ATGGCGCTGT TCAACCAGCA GTTGTGTGTT GGTTACCCAT CAGGCTTCAT GCGCTACAGT
    TTGTATGGAG AAACACCTCC AGCCAGCTTA CTTCATCCAG ATGACCCCAC ACTGGCATTT
    ATTAAAACAG ACAACCTGGA TGCCCTTTGT GCTGTGGAAA TCTCCAATAA GGAACTGCTG
    CTCTGCTTCA GTAAAATAGC AGTGTATGTT GACACACACG GCAGACGCTC GCGCCAGCAG
    GAGCTGATGT GGCCCGCGGC GCCCACCGCC TGCTGCTATA ACGCCCCCTA TCTGTCGGTC
    TACAGTGAAA ATGCAGTGGA TGTGTTTGAT GTGAACTCTA CGGAATGGAT TCAGACCATT
    CCTCTCAAAA AGGTCCGGCC ATTAAACATG GATGGCTCTT TGAATCTCCT GGGCCTAGAA
    ACGGTCAGAC TCATCTACTT CAGAAACAAG ACCGCAGAGG GGGATGAACT GGTGGTGCCT
    GAAGTGTGTG ATAACAGCAG AAAGCAGATG GTGCGAAGCA TGAGCAACAA GAGACGATTC
    TCGTTTCGTA TTCCTGAGGA GGAGAGACTG CAGCAGAGGA GAGAAATGTT GAAAGATCCT
    GAGAGGAGGA ACAAGCTGAT CTCAAATCCT ACAAATTTTA ACCATGTGGC TCATATGGGA
    CCTGGAGATG GCATGCAGAT ACTCAGAGAC CTGCCAATGA ATGTACGTGC TCAGGACAAC
    CGTGCTGGAT TCAGCGGCTC CGTCAGCATC CCCTCCATTA CAAAAAACAG AGCAGAACCA
    GGGCGATCCA TGAGCGCAAG CAGTGGCCTA GGAATGCGGT CGTCCTCTCA GAACGGCAGT
    GCTCTAAGGA GGGAGCTGTC CAGCGGCAGC TACAGCTCTA AACGGCAGAC CATGACTTCA
    CCCTCCGAGA GTTCGCTGTC ATCAGGAGGA GGGATGGACG TGGGGGAAGC GCCGCTCTCT
    CAGTTTGACA GAGAGGACTC TGATTCGCCG CGGCACTCCA CTGCCTCCAA CAGCTCCACC
    TTCAGCAGCC CGCCGAGCCC CGCCTCCCCA CACAAGACAA AGTCCCTCTC ATTGGAGAGC
    ACAGACCGCA CCGCCTGGGA CACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160808.1
    CDS1650..6956
    Translation

    Target ORF information:

    RefSeq Version XM_005160751.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005160751.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC
    TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG
    CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC
    CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT
    GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC
    TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA
    GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC
    GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA
    GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA
    CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT
    GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG
    ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC
    CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA
    GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG
    AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG
    AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC
    AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC
    AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT
    CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG
    CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC
    CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG
    GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG
    CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC
    AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC
    AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC
    GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG
    GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC
    ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG
    GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA
    ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG
    CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC
    AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC
    CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG
    AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT
    TCTGCACTGG ATCAGTTTGA TCGCTCACCC TCTTTTGGTC CCGGAAGCAA AACCAGACGT
    CTTGACTCTG CAGATTTCAC CCCTTCCAAC ACTCCATCCA GAGATGAAGA CGCAAGATCT
    CGCCGTATTT CACGCTCTCG CTCACCCTCC ACCGCCAGTG ACATGGAGCC TATAGAGTTG
    ATTGACCACA CATCACGTAG TCTTCAGACC CCAACAGGCA GATCAGGCTA CGGGAGCTTG
    GGACACTCAT CACCTAAGCC CAAGGCACAC CAGTTTAGTG TAAAGTCCTT CAATGTTCCC
    ACTAAATGTA ACCAGTGCAC CTCTCTCATG GTGGGACTCA TCCGTCAGGG CTGCACATGC
    GAAGCCTGCA GCTTCTCCTG TCATGTCACT TGTGCTGATA AAGCCCCTTC TGTGTGCCCC
    GTCGCACCAG ACCAGACCAG GGGCAAACTG GGCATTGACA CTCAGAGGGG CATTGGGACT
    GCATATGAGG GGCATCTTAG GGTGCCGAAG CCCACTGGGG TGAAGAAGGG TTGGCAGAGG
    GTCTGGGCAG TGGTTTGTGA CTTCAAACTT TTTCTGTATG AGCTTGGAGA AGGGAAAGGT
    GCTCAGCCTG GTGTTGTAGT AAACCAGGTC ATTGACATGA GGGATGAAGA GTTTTCCGCC
    AGTTCGGTTC TGGAGTCTGA TGTCATCCAT GCTAATAGGA AGGATATACC CTGTATTTTT
    AGGGTAACAG CATCTCAGCT GTCCGCCTCC TCCAGTCAGA AGACGTGTAT TCTAATTCTG
    GCTGACAACG AGCAAGAAAG GACGAAGTGG GTGTGCGCTC TGAATGAACT GCATCGCATC
    CTGAGGAAAA ACAAGCTGAA GGAGCGTTTC GTCTATGTGC CTAAAGAAGC CTACGACAGC
    ACACTTCCTC TCATCAAAAC CACCCAAACT GCTGCTATTA TAGATCATGA ACGCGTTGCT
    TTGGGCAATG AGGAGGGCCT GTTTGTCATC CACGTCACCA AAGATGAAAT CATTCGTGTG
    GGTGATGTCA AGAAGGTGCA TCATGTAGAA CTGATGCCCA CGGAGCACAT ACTGGCCGTC
    ATCTCGGGCA GGAACCGACA AGTATGTCTG GTCTCCATGG CGGGTCTCGA CGGGAGGGAG
    GTCGACAAGA ATAAGGTGGC AGATACTAAA AACTGCCAGT TGCTGGTGTC CGGTGTGGTT
    CGCAATGTCA CCTGCTTCTG CCTCGCCATT AAACGGCAGA TCTCATGCTT CGAAATTAAC
    AAGAGCAAGT CACGACACTC CCATCTCGTG GACATACAAG CTCCAGGAAC TGTCCAATGG
    ATGGCGCTGT TCAACCAGCA GTTGTGTGTT GGTTACCCAT CAGGCTTCAT GCGCTACAGT
    TTGTATGGAG AAACACCTCC AGCCAGCTTA CTTCATCCAG ATGACCCCAC ACTGGCATTT
    ATTAAAACAG ACAACCTGGA TGCCCTTTGT GCTGTGGAAA TCTCCAATAA GGAACTGCTG
    CTCTGCTTCA GTAAAATAGC AGTGTATGTT GACACACACG GCAGACGCTC GCGCCAGCAG
    GAGCTGATGT GGCCCGCGGC GCCCACCGCC TGCTGCTATA ACGCCCCCTA TCTGTCGGTC
    TACAGTGAAA ATGCAGTGGA TGTGTTTGAT GTGAACTCTA CGGAATGGAT TCAGACCATT
    CCTCTCAAAA AGGTCCGGCC ATTAAACATG GATGGCTCTT TGAATCTCCT GGGCCTAGAA
    ACGGTCAGAC TCATCTACTT CAGAAACAAG ACCGCAGAGG GGGATGAACT GGTGGTGCCT
    GAAGTGTGTG ATAACAGCAG AAAGCAGATG GTGCGAAGCA TGAGCAACAA GAGACGATTC
    TCGTTTCGTA TTCCTGAGGA GGAGAGACTG CAGCAGAGGA GAGAAATGTT GAAAGATCCT
    GAGAGGAGGA ACAAGCTGAT CTCAAATCCT ACAAATTTTA ACCATGTGGC TCATATGGGA
    CCTGGAGATG GCATGCAGAT ACTCAGAGAC CTGCCAATGA ATGTACGTGC TCAGGACAAC
    CGTGCTGGAT TCAGCGGCTC CGTCAGCATC CCCTCCATTA CAAAAAACAG AGCAGAACCA
    GGGCGATCCA TGAGCGCAAG CAGTGGCCTA GGAATGCGGT CGTCCTCTCA GAACGGCAGT
    GCTCTAAGGA GGGAGCTGTC CAGCGGCAGC TACAGCTCTA AACGGCAGAC CATGACTTCA
    CCCTCCGAGA GTTCGCTGTC ATCAGGAGGA GGGATGGACG TGGGGGAAGC GCCGCTCTCT
    CAGTTTGACA GAGAGGACTC TGATTCGCCG CGGCACTCCA CTGCCTCCAA CAGCTCCACC
    TTCAGCAGCC CGCCGAGCCC CGCCTCCCCA CACAAGACAA AGTCCCTCTC ATTGGAGAGC
    ACAGACCGCA CCGCCTGGGA CACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44774
    Clone ID Related Accession (Same CDS sequence) XM_009294768.2
    Accession Version XM_009294768.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5343bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product serine/threonine-protein kinase MRCK alpha isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294768.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC
    TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG
    CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC
    CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT
    GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC
    TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA
    GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC
    GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA
    GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA
    CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT
    GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG
    ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC
    CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA
    GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG
    AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG
    AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC
    AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC
    AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT
    CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG
    CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC
    CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG
    GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG
    CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC
    AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC
    AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC
    GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG
    GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC
    ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG
    GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA
    ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG
    CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC
    AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC
    CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG
    AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT
    TCTGCACTGG ATCAGTTTGA TCTTGACTCT GCAGATTTCA CCCCTTCCAA CACTCCATCC
    AGAGATGAAG ACGCAAGATC TCGCCGTATT TCACGCTCTC GCTCACCCTC CACCGCCAGT
    GACATGGAGC CTATAGAGTT GATTGACCAC ACATCACGTA GTCTTCAGAC CCCAACAGGC
    AGATCAGGCT ACGGGAGCTT GGGACACTCA TCACCTAAGC CCAAGGCACA CCAGTTTAGT
    GTAAAGTCCT TCAATGTTCC CACTAAATGT AACCAGTGCA CCTCTCTCAT GGTGGGACTC
    ATCCGTCAGG GCTGCACATG CGAAGCCTGC AGCTTCTCCT GTCATGTCAC TTGTGCTGAT
    AAAGCCCCTT CTGTGTGCCC CGTCGCACCA GACCAGACCA GGGGCAAACT GGGCATTGAC
    ACTCAGAGGG GCATTGGGAC TGCATATGAG GGGCATCTTA GGGTGCCGAA GCCCACTGGG
    GTGAAGAAGG GTTGGCAGAG GGTCTGGGCA GTGGTTTGTG ACTTCAAACT TTTTCTGTAT
    GAGCTTGGAG AAGGGAAAGG TGCTCAGCCT GGTGTTGTAG TAAACCAGGT CATTGACATG
    AGGGATGAAG AGTTTTCCGC CAGTTCGGTT CTGGAGTCTG ATGTCATCCA TGCTAATAGG
    AAGGATATAC CCTGTATTTT TAGGGTAACA GCATCTCAGC TGTCCGCCTC CTCCAGTCAG
    AAGACGTGTA TTCTAATTCT GGCTGACAAC GAGCAAGAAA GGACGAAGTG GGTGTGCGCT
    CTGAATGAAC TGCATCGCAT CCTGAGGAAA AACAAGCTGA AGGAGCGTTT CGTCTATGTG
    CCTAAAGAAG CCTACGACAG CACACTTCCT CTCATCAAAA CCACCCAAAC TGCTGCTATT
    ATAGATCATG AACGCGTTGC TTTGGGCAAT GAGGAGGGCC TGTTTGTCAT CCACGTCACC
    AAAGATGAAA TCATTCGTGT GGGTGATGTC AAGAAGGTGC ATCATGTAGA ACTGATGCCC
    ACGGAGCACA TACTGGCCGT CATCTCGGGC AGGAACCGAC AAGTATGTCT GGTCTCCATG
    GCGGGTCTCG ACGGGAGGGA GGTCGACAAG AATAAGGTGG CAGATACTAA AAACTGCCAG
    TTGCTGGTGT CCGGTGTGGT TCGCAATGTC ACCTGCTTCT GCCTCGCCAT TAAACGGCAG
    ATCTCATGCT TCGAAATTAA CAAGAGCAAG TCACGACACT CCCATCTCGT GGACATACAA
    GCTCCAGGAA CTGTCCAATG GATGGCGCTG TTCAACCAGC AGTTGTGTGT TGGTTACCCA
    TCAGGCTTCA TGCGCTACAG TTTGTATGGA GAAACACCTC CAGCCAGCTT ACTTCATCCA
    GATGACCCCA CACTGGCATT TATTAAAACA GACAACCTGG ATGCCCTTTG TGCTGTGGAA
    ATCTCCAATA AGGAACTGCT GCTCTGCTTC AGTAAAATAG CAGTGTATGT TGACACACAC
    GGCAGACGCT CGCGCCAGCA GGAGCTGATG TGGCCCGCGG CGCCCACCGC CTGCTGCTAT
    AACGCCCCCT ATCTGTCGGT CTACAGTGAA AATGCAGTGG ATGTGTTTGA TGTGAACTCT
    ACGGAATGGA TTCAGACCAT TCCTCTCAAA AAGGTCCGGC CATTAAACAT GGATGGCTCT
    TTGAATCTCC TGGGCCTAGA AACGGTCAGA CTCATCTACT TCAGAAACAA GACCGCAGAG
    GGGGATGAAC TGGTGGTGCC TGAAGTGTGT GATAACAGCA GAAAGCAGAT GGTGCGAAGC
    ATGAGCAACA AGAGACGATT CTCGTTTCGT ATTCCTGAGG AGGAGAGACT GCAGCAGAGG
    AGAGAAATGT TGAAAGATCC TGAGAGGAGG AACAAGCTGA TCTCAAATCC TACAAATTTT
    AACCATGTGG CTCATATGGG ACCTGGAGAT GGCATGCAGA TACTCAGAGA CCTGCCAATG
    AATGTACGTG CTCAGGACAA CCGTGCTGGA TTCAGCGGCT CCGTCAGCAT CCCCTCCATT
    ACAAAAAACA GAGCAGAACC AGGGCGATCC ATGAGCGCAA GCAGTGGCCT AGGAATGCGG
    TCGTCCTCTC AGAACGGCAG TGCTCTAAGG AGGGAGCTGT CCAGCGGCAG CTACAGCTCT
    AAACGGCAGA CCATGACTTC ACCCTCCGAG AGTTCGCTGT CATCAGGAGG AGGGATGGAC
    GTGGGGGAAG CGCCGCTCTC TCAGTTTGAC AGAGAGTGGC AGGCAGTATC GCCCTCGGAG
    AAGACCCCTG ATCTGAAAAG GGCTTTAAGG ACCCTGCTTA GTAAGATGAA GGACTCTGAT
    TCGCCGCGGC ACTCCACTGC CTCCAACAGC TCCACCTTCA GCAGCCCGCC GAGCCCCGCC
    TCCCCACACA AGACAAAGTC CCTCTCATTG GAGAGCACAG ACCGCACCGC CTGGGACACA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293043.1
    CDS1650..6992
    Translation

    Target ORF information:

    RefSeq Version XM_009294768.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:ch211-89p3.3 (si:ch211-89p3.3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294768.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCTGTGG AGGTGCGTTT AAAGAAGCTG GAGAAACTGA TTATGGATGG CCCGTCTCAG 
    TCCAGCGGCC AGTGCTTCAG CATGGAGACC TTGTTGGATA TCTTAGTTTG CCTGTACGAC
    GAGTGCAGCA GTTCTCCCAT CAGAAGAGAG AAGAACGTCC TGGAGTTTCT AGACTGGGCC
    AAACCTTTCA CATCAAAGGT CAAACAGATG CGCTTACACA AGGAGGACTT TGAGATCCTG
    AAGGTGATTG GACGGGGAGC TTTTGGAGAG GTTGCAGTAG TAAAAGTGAA AAACACAGAC
    AAAGTATTTG CCATGAAGAT TCTCAACAAA TGGGAAATGC TCAAAAGGGC TGAGACGGCA
    TGTTTTCGGG AGGAGCGGGA CGTGCTGGTG AATGGGGACT GTCAGTGGAT CACGACATTA
    CACTATGCTT TCCAGGACGA GAACAACTTG TACCTGGTGA TGGACTACTA TGTAGGGGGA
    GATCTGTTGA CTCTGCTTAG TAAGTTTGAA GATCGTCTGC CTGAAGACAT GGCCAAGTTC
    TATCTGGCCG AGATGGTCTT GGCTATTGAC TCTGTGCATC AACTACATTA TGTCCACAGA
    GATATCAAAC CAGACAATAT CCTGATGGAC ATGAATGGTC ACATCCGGCT GGCTGACTTT
    GGCTCCTGCC TCAAACTCAT GGAAGACGGG ACAGTCCAGT CCTCTGTAGC AGTTGGGACC
    CCGGACTACA TCTCACCAGA GATCCTGCAG GCTATGGAGG ACGGGAAGGG AAAGTACGGG
    CCCGAGTGTG ACTGGTGGTC TCTGGGGGTC TGCATGTATG AGATGCTGTA TGGAGAGACG
    CCTTTTTATG CTGAATCTCT GGTGGAGACG TATGGAAAGA TCATGAACCA TAAGGAGCGC
    TTCCAGTTCC CACTTCAGGT GACTGATGTG TCCGAGGATG CTAAAGATCT TGTTCGGCGG
    CTGATCTGCA GCAGAGAACA CCGGCTGGGC CAGAACGGTA TCGAAGACTT TAAGCAGCAC
    CCCTTTTTCA CAGGCATCGA CTGGGACAAC ATCCGCACAT GCGAAGCCCC CTACATCCCT
    GAAGTCAGCA GTCCCACTGA CACCTCCAAC TTTGATGTGG ATGACGACTG CCTCAAAAAC
    TGTGAGACGT TGCCTCCACC TTCACACACA GCATTTTCTG GACACCACCT CCCATTCGTA
    GGCTTCACCT ACACGAGTAA ATGCACGCTG TCAGATCGAG GGTGTTTGCG GGAATCGGTC
    GGACCTGCAC AGGTTGATGC TCATGTTCAG CGTAGCCTGG AGGAGAGCCT GGCCTCTGAA
    GCCTATGAGA GGAGAATTCG ACGTTTAGAG CAGGAGAAAA CCGAACTTAC CCGTAAACTA
    CAGGAGTCTA CGCAAACGGT GCAAGCACTG CAGTACCCTG ATTCAGACGC CCCAGTGAAT
    GCTAATAAAG AGGTAGAGAT AAGGAGTTTA AAGAGTGAGA TCGACATCCT GAAGAAACAG
    ATAGCAGACT CTGGTCAGAT GGAGCAGCAG TTGGAGAACG CCAGCACAGC TAGAAGAGAC
    CTGGAAGACT CGTCCAAGGT CGTCAGGAAC TTAGAGAAAC AGCTGAAGAG TGTCACACAA
    GAGAAAGATG ATCTGCACAA GGAGCTGCTG GGTTTGGCAG AGAAGGTGAA GGCTCAGGGG
    AAGGAGCTAA AGGATGCTCA CAGTCAAAAA AAGATGGCCA TAAAGGAGTT CGCTGAGCTG
    AATGAACAGC TGAGCGATGT TCGCACACAA AAGCAGCGCC TCAGCCGACA GTTCAAAGAC
    AAGGAGGAGG AGATGGAAGG AGTGTCGCAG AAACTAGAGG CTCTACGGCT AGAGATACGC
    AAAGCCGAGA GAATGCGCAA AGAGTTGGAG GCCCAGGCTG AGGAACATGC AGCAGAAGCT
    CAGAAGGAGA GAAAGGTTCG AGAAAGAACA GAACAGTACA GCAAACAACT AGAGGAGGAG
    CTCGAGGGCA TGAAGCAGTT GAAGCAGACT GGGCCTCCTA ACGGAGCTGC AAGCTCAGAC
    CAGCAGCAGG AGCTGATGCG CGTTCGGGCT GAACTGGAGA AGAAGAGCGT ACAGTACGAG
    GAGGAGATGT GTCGCAGAGA GACGCTCCAC AGCAGTGAGA TGAAGAACCT CCAAAAAGAG
    CTACGGGATG CCGAGGGTCA ACACCTCACA CATCAGAAAG AGATACTCGT GCTTAAAGAC
    AAACTGGAGA AAACTCGCAG AGAAAGTCAA GTCGAGCGAG AAGAATTTGT GTGCGAGTAC
    AAGCAGAAGT ACGAGAGGGA AAAAGCCCAT CTGACAGACG AAAACAAGAA GCTGAGTGCC
    GAAGTGGAGA AGTTGAACGG TCAGTTCGAG CAGCTGAGCA GCAGTCACCG GCAGCTGGAG
    GAGGAGATGA GAGAACTGGC TGATAAGAAG GAGAGTGTTG CCCACTGGGA GGCCCAGATC
    ACCGAGATCA TCCAGTGGGT GAGCGATGAG AAGGATGCCC GTGGTTACCT TCAAGCTCTG
    GCCACCAAAA TGACAGAGGA GCTGGAAGGG CTCAGAAACA CCAGTTTGGG AGCCAGAGGA
    ACGGACATGC CGTGGAAGAT GAGGCGTTTC GCCAAGCTGG ACATGTCTGC GCGTCTGGAG
    CTGCAGTCGG CGCTGGATAC TGAGATCAGA GCCAAACAGT CCATCCAAGA CGAGCTTAAC
    AAAGTCAAAG CAACCAACAT TGCCACAGAA TGCAAATTGC AAGAATCCGA AAGTAGAAAC
    CAGGAGCTGC TGGGAGAAAT TGAGAGACTG AGGAAGGAGA CAGAGGAGCT AAGGCTGAGG
    AGAGGCGTGA AGCACCAGGA TTCCCAAAAT TCCTTTTTGG CTTTCCTCAA TGCGCCCACT
    TCTGCACTGG ATCAGTTTGA TCTTGACTCT GCAGATTTCA CCCCTTCCAA CACTCCATCC
    AGAGATGAAG ACGCAAGATC TCGCCGTATT TCACGCTCTC GCTCACCCTC CACCGCCAGT
    GACATGGAGC CTATAGAGTT GATTGACCAC ACATCACGTA GTCTTCAGAC CCCAACAGGC
    AGATCAGGCT ACGGGAGCTT GGGACACTCA TCACCTAAGC CCAAGGCACA CCAGTTTAGT
    GTAAAGTCCT TCAATGTTCC CACTAAATGT AACCAGTGCA CCTCTCTCAT GGTGGGACTC
    ATCCGTCAGG GCTGCACATG CGAAGCCTGC AGCTTCTCCT GTCATGTCAC TTGTGCTGAT
    AAAGCCCCTT CTGTGTGCCC CGTCGCACCA GACCAGACCA GGGGCAAACT GGGCATTGAC
    ACTCAGAGGG GCATTGGGAC TGCATATGAG GGGCATCTTA GGGTGCCGAA GCCCACTGGG
    GTGAAGAAGG GTTGGCAGAG GGTCTGGGCA GTGGTTTGTG ACTTCAAACT TTTTCTGTAT
    GAGCTTGGAG AAGGGAAAGG TGCTCAGCCT GGTGTTGTAG TAAACCAGGT CATTGACATG
    AGGGATGAAG AGTTTTCCGC CAGTTCGGTT CTGGAGTCTG ATGTCATCCA TGCTAATAGG
    AAGGATATAC CCTGTATTTT TAGGGTAACA GCATCTCAGC TGTCCGCCTC CTCCAGTCAG
    AAGACGTGTA TTCTAATTCT GGCTGACAAC GAGCAAGAAA GGACGAAGTG GGTGTGCGCT
    CTGAATGAAC TGCATCGCAT CCTGAGGAAA AACAAGCTGA AGGAGCGTTT CGTCTATGTG
    CCTAAAGAAG CCTACGACAG CACACTTCCT CTCATCAAAA CCACCCAAAC TGCTGCTATT
    ATAGATCATG AACGCGTTGC TTTGGGCAAT GAGGAGGGCC TGTTTGTCAT CCACGTCACC
    AAAGATGAAA TCATTCGTGT GGGTGATGTC AAGAAGGTGC ATCATGTAGA ACTGATGCCC
    ACGGAGCACA TACTGGCCGT CATCTCGGGC AGGAACCGAC AAGTATGTCT GGTCTCCATG
    GCGGGTCTCG ACGGGAGGGA GGTCGACAAG AATAAGGTGG CAGATACTAA AAACTGCCAG
    TTGCTGGTGT CCGGTGTGGT TCGCAATGTC ACCTGCTTCT GCCTCGCCAT TAAACGGCAG
    ATCTCATGCT TCGAAATTAA CAAGAGCAAG TCACGACACT CCCATCTCGT GGACATACAA
    GCTCCAGGAA CTGTCCAATG GATGGCGCTG TTCAACCAGC AGTTGTGTGT TGGTTACCCA
    TCAGGCTTCA TGCGCTACAG TTTGTATGGA GAAACACCTC CAGCCAGCTT ACTTCATCCA
    GATGACCCCA CACTGGCATT TATTAAAACA GACAACCTGG ATGCCCTTTG TGCTGTGGAA
    ATCTCCAATA AGGAACTGCT GCTCTGCTTC AGTAAAATAG CAGTGTATGT TGACACACAC
    GGCAGACGCT CGCGCCAGCA GGAGCTGATG TGGCCCGCGG CGCCCACCGC CTGCTGCTAT
    AACGCCCCCT ATCTGTCGGT CTACAGTGAA AATGCAGTGG ATGTGTTTGA TGTGAACTCT
    ACGGAATGGA TTCAGACCAT TCCTCTCAAA AAGGTCCGGC CATTAAACAT GGATGGCTCT
    TTGAATCTCC TGGGCCTAGA AACGGTCAGA CTCATCTACT TCAGAAACAA GACCGCAGAG
    GGGGATGAAC TGGTGGTGCC TGAAGTGTGT GATAACAGCA GAAAGCAGAT GGTGCGAAGC
    ATGAGCAACA AGAGACGATT CTCGTTTCGT ATTCCTGAGG AGGAGAGACT GCAGCAGAGG
    AGAGAAATGT TGAAAGATCC TGAGAGGAGG AACAAGCTGA TCTCAAATCC TACAAATTTT
    AACCATGTGG CTCATATGGG ACCTGGAGAT GGCATGCAGA TACTCAGAGA CCTGCCAATG
    AATGTACGTG CTCAGGACAA CCGTGCTGGA TTCAGCGGCT CCGTCAGCAT CCCCTCCATT
    ACAAAAAACA GAGCAGAACC AGGGCGATCC ATGAGCGCAA GCAGTGGCCT AGGAATGCGG
    TCGTCCTCTC AGAACGGCAG TGCTCTAAGG AGGGAGCTGT CCAGCGGCAG CTACAGCTCT
    AAACGGCAGA CCATGACTTC ACCCTCCGAG AGTTCGCTGT CATCAGGAGG AGGGATGGAC
    GTGGGGGAAG CGCCGCTCTC TCAGTTTGAC AGAGAGTGGC AGGCAGTATC GCCCTCGGAG
    AAGACCCCTG ATCTGAAAAG GGCTTTAAGG ACCCTGCTTA GTAAGATGAA GGACTCTGAT
    TCGCCGCGGC ACTCCACTGC CTCCAACAGC TCCACCTTCA GCAGCCCGCC GAGCCCCGCC
    TCCCCACACA AGACAAAGTC CCTCTCATTG GAGAGCACAG ACCGCACCGC CTGGGACACA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.