CDC42BPA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CDC42BPA
Entrez Gene ID	8476
Full Name	CDC42 binding protein kinase alpha
Synonyms	MRCK,MRCKA,PK428
General protein information	Preferred Names CDC42 binding protein kinase alpha Names serine/threonine-protein kinase MRCK alpha CDC42 binding protein kinase alpha (DMPK-like) CDC42 binidng protein kinase beta DMPK-like alpha MRCK alpha myotonic dystrophy kinase-related CDC42-binding kinase alpha myotonic dystrophy kinase-related CDC42-binding protein kinase alpha myotonic dystrophy protein kinase-like alpha ser-thr protein kinase PK428 ser-thr protein kinase related to the myotonic dystrophy protein kinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q42.13
Summary	The protein encoded by this gene is a member of the Serine/Threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described, and the full-length nature of two of them has been reported. [provided by RefSeq, Jul 2008].
Disorder MIM:	603412

mRNA and Protein(s)

mRNA	Protein	Name
XM_011544308.2	XP_011542610.1	serine/threonine-protein kinase MRCK alpha isoform X8
XM_017002578.1	XP_016858067.1	serine/threonine-protein kinase MRCK alpha isoform X10
XM_017002579.1	XP_016858068.1	serine/threonine-protein kinase MRCK alpha isoform X13
XM_017002581.1	XP_016858070.1	serine/threonine-protein kinase MRCK alpha isoform X15
XM_017002582.1	XP_016858071.1	serine/threonine-protein kinase MRCK alpha isoform X16
XM_011544307.2	XP_011542609.1	serine/threonine-protein kinase MRCK alpha isoform X5
NM_001366019.1	NP_001352948.1	serine/threonine-protein kinase MRCK alpha isoform C
NM_003607.3	NP_003598.2	serine/threonine-protein kinase MRCK alpha isoform B
XM_011544306.3	XP_011542608.1	serine/threonine-protein kinase MRCK alpha isoform X1
XM_005273318.4	XP_005273375.1	serine/threonine-protein kinase MRCK alpha isoform X3
XM_017002581.2	XP_016858070.1	serine/threonine-protein kinase MRCK alpha isoform X15
NM_001366010.1	NP_001352939.1	serine/threonine-protein kinase MRCK alpha isoform D
XM_005273318.3	XP_005273375.1	serine/threonine-protein kinase MRCK alpha isoform X3
XM_006711834.3	XP_006711897.1	serine/threonine-protein kinase MRCK alpha isoform X4
NM_001366019.2	NP_001352948.1	serine/threonine-protein kinase MRCK alpha isoform C
XM_011544308.3	XP_011542610.1	serine/threonine-protein kinase MRCK alpha isoform X8
XM_005273322.4	XP_005273379.1	serine/threonine-protein kinase MRCK alpha isoform X12
XM_017002580.2	XP_016858069.1	serine/threonine-protein kinase MRCK alpha isoform X13
XM_017002580.1	XP_016858069.1	serine/threonine-protein kinase MRCK alpha isoform X13
XM_011544306.2	XP_011542608.1	serine/threonine-protein kinase MRCK alpha isoform X1
XM_011544309.2	XP_011542611.1	serine/threonine-protein kinase MRCK alpha isoform X11
XM_005273317.3	XP_005273374.1	serine/threonine-protein kinase MRCK alpha isoform X2
NM_001366010.2	NP_001352939.1	serine/threonine-protein kinase MRCK alpha isoform D
XM_011544309.3	XP_011542611.1	serine/threonine-protein kinase MRCK alpha isoform X11
XM_017002578.2	XP_016858067.1	serine/threonine-protein kinase MRCK alpha isoform X10
XM_006711835.3	XP_006711898.1	serine/threonine-protein kinase MRCK alpha isoform X6
XM_005273324.3	XP_005273381.1	serine/threonine-protein kinase MRCK alpha isoform X14
XM_005273320.3	XP_005273377.1	serine/threonine-protein kinase MRCK alpha isoform X7
XM_005273321.3	XP_005273378.1	serine/threonine-protein kinase MRCK alpha isoform X9
XM_005273322.3	XP_005273379.1	serine/threonine-protein kinase MRCK alpha isoform X12
NM_014826.4	NP_055641.3	serine/threonine-protein kinase MRCK alpha isoform A
XM_006711835.4	XP_006711898.1	serine/threonine-protein kinase MRCK alpha isoform X6
XM_005273320.4	XP_005273377.1	serine/threonine-protein kinase MRCK alpha isoform X7
XM_005273321.4	XP_005273378.1	serine/threonine-protein kinase MRCK alpha isoform X9
XM_017002579.2	XP_016858068.1	serine/threonine-protein kinase MRCK alpha isoform X13
XM_017002582.2	XP_016858071.1	serine/threonine-protein kinase MRCK alpha isoform X16
NM_014826.5	NP_055641.3	serine/threonine-protein kinase MRCK alpha isoform A
NM_001366011.1	NP_001352940.1	serine/threonine-protein kinase MRCK alpha isoform E
XM_005273317.4	XP_005273374.1	serine/threonine-protein kinase MRCK alpha isoform X2
XM_006711834.4	XP_006711897.1	serine/threonine-protein kinase MRCK alpha isoform X4
XM_011544307.3	XP_011542609.1	serine/threonine-protein kinase MRCK alpha isoform X5
NM_003607.4	NP_003598.2	serine/threonine-protein kinase MRCK alpha isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	CDC42BPA	NP_003598.2
	CDC42BPA	XP_002801950.1
Canis lupus familiaris (dog)	CDC42BPA	XP_005622674.1
Gallus gallus (chicken)	CDC42BPA	NP_001186372.1
Xenopus tropicalis (tropical clawed frog)	cdc42bpa	XP_002938889.2
Pan troglodytes (chimpanzee)	CDC42BPA	XP_003949757.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012090	XP_320436.4
Rattus norvegicus (Norway rat)	Cdc42bpa	NP_446109.1
Danio rerio (zebrafish)	si:ch211-89p3.3	NP_001038559.1
Drosophila melanogaster (fruit fly)	gek	NP_523837.2
Caenorhabditis elegans (roundworm)	mrck-1	NP_504599.2
Mus musculus (house mouse)	Cdc42bpa	NP_001028457.1

Gene Ontology
Bibliography

Related articles in PubMed

MRCK? is activated by caspase cleavage to assemble an apical actin ring for epithelial cell extrusion.
Gagliardi PA, Somale D, Puliafito A, Chiaverina G, di Blasio L, Oneto M, Bianchini P, Bussolino F, Primo L
The Journal of cell biology217(1)231-249(2018 Jan)

Comparative Proteomics Analysis Identifies Cdc42-Cdc42BPA Signaling as Prognostic Biomarker and Therapeutic Target for Colon Cancer Invasion.
Hu HF, Xu WW, Wang Y, Zheng CC, Zhang WX, Li B, He QY
Journal of proteome research17(1)265-275(2018 Jan)

Inhibition of cell migration and invasion by miR?29a?3p in a colorectal cancer cell line through suppression of CDC42BPA mRNA expression.
He PY, Yip WK, Chai BL, Chai BY, Jabar MF, Dusa N, Mohtarrudin N, Seow HF
Oncology reports38(6)3554-3566(2017 Dec)

ICAP-1 monoubiquitylation coordinates matrix density and rigidity sensing for cell migration through ROCK2-MRCK? balance.
Bouin AP, Kyumurkov A, R?gent-Kloeckner M, Ribba AS, Faurobert E, Fournier HN, Bourrin-Reynard I, Manet-Dup? S, Oddou C, Balland M, Planus E, Albiges-Rizo C
Journal of cell science130(3)626-636(2017 02)

Discovery and characterization of a novel CCND1/MRCK gene fusion in mantle cell lymphoma.
Masamha CP, Albrecht TR, Wagner EJ
Journal of hematology & oncology930(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ICAP-1 monoubiquitylation helps in switching from ROCK2-mediated to MRCKalpha-mediated cell contractility.
Title: ICAP-1 monoubiquitylation coordinates matrix density and rigidity sensing for cell migration through ROCK2-MRCK? balance.

Data show that the fusion gene cyclin D1/myotonic dystrophy kinase-related CDC42-binding protein kinase alpha (CCND1/MRCK) mRNA is resistant to CCND1-targeted miRNA regulation.
Title: Discovery and characterization of a novel CCND1/MRCK gene fusion in mantle cell lymphoma.

the functional relevance of the LRAP25-MRCK complex in LIMK1-cofilin signaling and the importance of LRAP adaptors as key determinants of MRCK cellular localization and downstream specificities.
Title: Adaptor protein LRAP25 mediates myotonic dystrophy kinase-related Cdc42-binding kinase (MRCK) regulation of LIMK1 protein in lamellipodial F-actin dynamics.

A functional pathway involving PDK1-mediated activation of MRCKA, links EGF signaling to myosin contraction and directional migration.
Title: PDK1-mediated activation of MRCK? regulates directional cell migration and lamellipodia retraction.

MRCK participates in mediating the effects of HMI-1a3 on HeLa cell morphology.
Title: Evidence for a role of MRCK in mediating HeLa cell elongation induced by the C1 domain ligand HMI-1a3.

The following CDC42BPA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDC42BPA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03424	NM_003607.4 Latest version!	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu107134	NM_001366010.2 Latest version!	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu107744	NM_001366011.1 Latest version!	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu90668	XM_017002578.2 Latest version!	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu90669	XM_017002580.2 Latest version!	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu90669	XM_017002579.2 Latest version!	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu90670	XM_017002581.2 Latest version!	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu90671	XM_017002582.2 Latest version!	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu06683	NM_014826.5 Latest version!	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu35146	NM_001366019.2 Latest version!	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu35137	XM_005273317.4 Latest version!	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu35138	XM_005273318.4 Latest version!	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu35139	XM_006711834.4 Latest version!	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu35140	XM_006711835.4 Latest version!	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu35141	XM_005273320.4 Latest version!	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu35142	XM_005273321.4 Latest version!	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu35144	XM_005273322.4 Latest version!	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu35146	XM_005273324.3 Latest version!	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu61833	XM_011544306.3 Latest version!	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu61834	XM_011544307.3 Latest version!	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu61835	XM_011544308.3 Latest version!	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu61836	XM_011544309.3 Latest version!	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OHu90670	XM_017002581.1	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu35138	XM_005273318.3	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu35146	NM_001366019.1	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu61835	XM_011544308.2	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu90669	XM_017002580.1	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu61833	XM_011544306.2	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu107134	NM_001366010.1	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu61836	XM_011544309.2	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu90668	XM_017002578.1	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu35144	XM_005273322.3	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu35140	XM_006711835.3	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu35141	XM_005273320.3	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu35142	XM_005273321.3	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu90669	XM_017002579.1	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu90671	XM_017002582.1	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu06683	NM_014826.4	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu35137	XM_005273317.3	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu35139	XM_006711834.3	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu61834	XM_011544307.2	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu03424	NM_003607.3	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CDC42BPA ( NM_003607.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003598.2 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant B, mRNA.

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CloneID	OHu03424
Clone ID Related Accession (Same CDS sequence)	NM_003607.3 , NM_003607.4
Accession Version	NM_003607.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL627308.20, BQ232716.1, AJ518975.1, BX648219.1, BF765241.1, BC050335.1 and AL353689.26. On Jun 2, 2019 this sequence version replaced NM_003607.3. Transcript Variant: This variant (B) encodes the longer isoform (B). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ518975.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCCACTC CACTTTCAGT TCACACACCA 
ACCTTAAGGA AAAAAGGATG TCCTGGTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAC 
CAGTTTTTTG TAAAATCTTT TACTACTCCT ACCAAGTGTC ATCAGTGTAC CTCCTTGATG 
GTGGGTTTAA TAAGACAGGG CTGTTCATGT GAAGTGTGTG GATTCTCATG CCATATAACT 
TGTGTAAACA AAGCTCCAAC CACTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCCCTG 
GGTATAGATC CTCAGAAAGG AATAGGAACA GCATATGAAG GTCATGTCAG GATTCCTAAG 
CCAGCTGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTA TAGTGTGTGA CTTCAAACTC 
TTTCTGTACG ATATTGCTGA AGGAAAAGCA TCTCAGCCCA GTGTTGTCAT TAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATCCAT 
GCAAGTCGGA AAGATATACC CTGTATATTT AGGGTCACAG CTTCCCAGCT CTCAGCATCT 
AATAACAAAT GTTCAATCCT GATGCTAGCA GACACTGAGA ATGAGAAGAA TAAGTGGGTG 
GGAGTGCTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTACCCCTCA TTAAAACAAC CCAGGCAGCC 
GCAATCATAG ATCATGAAAG AATTGCTTTG GGAAACGAAG AAGGGTTATT TGTTGTACAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATGTCAG CATTGGATGG GCGAGAGACC GATTTTTACA AGCTGTCAGA AACTAAAGGG 
TGTCAAACCG TAACTTCTGG AAAGGTGCGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGAAAATTT 
AAAGAAATTC AAGTCCCATA TAATGTCCAG TGGATGGCAA TCTTCAGTGA ACAACTCTGT 
GTGGGATTCC AGTCAGGATT TCTAAGATAC CCCTTGAATG GAGAAGGAAA TCCATACAGT 
ATGCTCCATT CAAATGACCA TACACTATCA TTTATTGCAC ATCAACCAAT GGATGCTATC 
TGCGCAGTTG AGATCTCCAG TAAAGAATAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAATTGA TGTGGCCAGC AAATCCTTCC 
TCTTGTTGTT ACAATGCACC ATATCTCTCG GTGTACAGTG AAAATGCAGT TGATATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTTCCTCTCA AAAAGGTTCG ACCCTTAAAC 
AATGAAGGAT CATTAAATCT TTTAGGGTTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGACGA ACTGGTAGTA CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ACATTAACAA TAAGCGGCGT TATTCCTTCA GAGTCCCAGA AGAGGAAAGG 
ATGCAGCAGA GGAGGGAAAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAT 
CCAACTAATT TTAATCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTGAAA 
GATCTGCCCA TGAACCCTCG GCCTCAGGAA AGTCGGACAG TATTCAGTGG CTCAGTCAGT 
ATTCCATCTA TCACCAAATC CCGCCCTGAG CCAGGCCGCT CCATGAGTGC TAGCAGTGGC 
TTGTCAGCAA GGTCATCCGC ACAGAATGGC AGCGCATTAA AGAGGGAATT CTCTGGAGGA 
AGCTACAGTG CCAAGCGGCA GCCCATGCCC TCCCCGTCAG AGGGCTCTTT GTCCTCTGGA 
GGCATGGACC AAGGAAGTGA TGCCCCAGCG AGGGACTTTG ACGGAGAGGA CTCTGACTCT 
CCGAGGCATT CCACAGCTTC CAACAGTTCC AACCTAAGCA GCCCCCCAAG CCCAGCTTCA 
CCCCGAAAAA CCAAGAGCCT CTCCCTGGAG AGCACTGACC GCGGGAGCTG GGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003598.2
CDS	1311..6470
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	NM_003607.4
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003607.4

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCCACTC CACTTTCAGT TCACACACCA 
ACCTTAAGGA AAAAAGGATG TCCTGGTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAC 
CAGTTTTTTG TAAAATCTTT TACTACTCCT ACCAAGTGTC ATCAGTGTAC CTCCTTGATG 
GTGGGTTTAA TAAGACAGGG CTGTTCATGT GAAGTGTGTG GATTCTCATG CCATATAACT 
TGTGTAAACA AAGCTCCAAC CACTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCCCTG 
GGTATAGATC CTCAGAAAGG AATAGGAACA GCATATGAAG GTCATGTCAG GATTCCTAAG 
CCAGCTGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTA TAGTGTGTGA CTTCAAACTC 
TTTCTGTACG ATATTGCTGA AGGAAAAGCA TCTCAGCCCA GTGTTGTCAT TAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATCCAT 
GCAAGTCGGA AAGATATACC CTGTATATTT AGGGTCACAG CTTCCCAGCT CTCAGCATCT 
AATAACAAAT GTTCAATCCT GATGCTAGCA GACACTGAGA ATGAGAAGAA TAAGTGGGTG 
GGAGTGCTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTACCCCTCA TTAAAACAAC CCAGGCAGCC 
GCAATCATAG ATCATGAAAG AATTGCTTTG GGAAACGAAG AAGGGTTATT TGTTGTACAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATGTCAG CATTGGATGG GCGAGAGACC GATTTTTACA AGCTGTCAGA AACTAAAGGG 
TGTCAAACCG TAACTTCTGG AAAGGTGCGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGAAAATTT 
AAAGAAATTC AAGTCCCATA TAATGTCCAG TGGATGGCAA TCTTCAGTGA ACAACTCTGT 
GTGGGATTCC AGTCAGGATT TCTAAGATAC CCCTTGAATG GAGAAGGAAA TCCATACAGT 
ATGCTCCATT CAAATGACCA TACACTATCA TTTATTGCAC ATCAACCAAT GGATGCTATC 
TGCGCAGTTG AGATCTCCAG TAAAGAATAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAATTGA TGTGGCCAGC AAATCCTTCC 
TCTTGTTGTT ACAATGCACC ATATCTCTCG GTGTACAGTG AAAATGCAGT TGATATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTTCCTCTCA AAAAGGTTCG ACCCTTAAAC 
AATGAAGGAT CATTAAATCT TTTAGGGTTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGACGA ACTGGTAGTA CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ACATTAACAA TAAGCGGCGT TATTCCTTCA GAGTCCCAGA AGAGGAAAGG 
ATGCAGCAGA GGAGGGAAAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAT 
CCAACTAATT TTAATCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTGAAA 
GATCTGCCCA TGAACCCTCG GCCTCAGGAA AGTCGGACAG TATTCAGTGG CTCAGTCAGT 
ATTCCATCTA TCACCAAATC CCGCCCTGAG CCAGGCCGCT CCATGAGTGC TAGCAGTGGC 
TTGTCAGCAA GGTCATCCGC ACAGAATGGC AGCGCATTAA AGAGGGAATT CTCTGGAGGA 
AGCTACAGTG CCAAGCGGCA GCCCATGCCC TCCCCGTCAG AGGGCTCTTT GTCCTCTGGA 
GGCATGGACC AAGGAAGTGA TGCCCCAGCG AGGGACTTTG ACGGAGAGGA CTCTGACTCT 
CCGAGGCATT CCACAGCTTC CAACAGTTCC AACCTAAGCA GCCCCCCAAG CCCAGCTTCA 
CCCCGAAAAA CCAAGAGCCT CTCCCTGGAG AGCACTGACC GCGGGAGCTG GGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( NM_001366010.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001352939.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant D, mRNA.

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CloneID	OHu107134
Clone ID Related Accession (Same CDS sequence)	NM_001366010.1 , NM_001366010.2
Accession Version	NM_001366010.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-03
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL627308.20, AB208968.1, BC136333.1 and AL353689.26. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB208968.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCAAG ACTCACCAGT TTTTTGTAAA ATCTTTTACT 
ACTCCTACCA AGTGTCATCA GTGTACCTCC TTGATGGTGG GTTTAATAAG ACAGGGCTGT 
TCATGTGAAG TGTGTGGATT CTCATGCCAT ATAACTTGTG TAAACAAAGC TCCAACCACT 
TGTCCAGTTC CTCCTGAACA GACAAAAGGT CCCCTGGGTA TAGATCCTCA GAAAGGAATA 
GGAACAGCAT ATGAAGGTCA TGTCAGGATT CCTAAGCCAG CTGGAGTGAA GAAAGGGTGG 
CAGAGAGCAC TGGCTATAGT GTGTGACTTC AAACTCTTTC TGTACGATAT TGCTGAAGGA 
AAAGCATCTC AGCCCAGTGT TGTCATTAGT CAAGTGATTG ACATGAGGGA TGAAGAATTT 
TCTGTGAGTT CAGTCTTGGC TTCTGATGTT ATCCATGCAA GTCGGAAAGA TATACCCTGT 
ATATTTAGGG TCACAGCTTC CCAGCTCTCA GCATCTAATA ACAAATGTTC AATCCTGATG 
CTAGCAGACA CTGAGAATGA GAAGAATAAG TGGGTGGGAG TGCTGAGTGA ATTGCACAAG 
ATTTTGAAGA AAAACAAATT CAGAGACCGC TCAGTCTATG TTCCCAAAGA GGCTTATGAC 
AGCACTCTAC CCCTCATTAA AACAACCCAG GCAGCCGCAA TCATAGATCA TGAAAGAATT 
GCTTTGGGAA ACGAAGAAGG GTTATTTGTT GTACATGTCA CCAAAGATGA AATTATTAGA 
GTTGGTGACA ATAAGAAGAT TCATCAGATT GAACTCATTC CAAATGATCA GCTTGTTGCT 
GTGATCTCAG GACGAAATCG TCATGTACGA CTTTTTCCTA TGTCAGCATT GGATGGGCGA 
GAGACCGATT TTTACAAGCT GTCAGAAACT AAAGGGTGTC AAACCGTAAC TTCTGGAAAG 
GTGCGCCATG GAGCTCTCAC ATGCCTGTGT GTGGCTATGA AAAGGCAGGT CCTCTGTTAT 
GAACTATTTC AGAGCAAGAC CCGTCACAGA AAATTTAAAG AAATTCAAGT CCCATATAAT 
GTCCAGTGGA TGGCAATCTT CAGTGAACAA CTCTGTGTGG GATTCCAGTC AGGATTTCTA 
AGATACCCCT TGAATGGAGA AGGAAATCCA TACAGTATGC TCCATTCAAA TGACCATACA 
CTATCATTTA TTGCACATCA ACCAATGGAT GCTATCTGCG CAGTTGAGAT CTCCAGTAAA 
GAATATCTGC TGTGTTTTAA CAGCATTGGG ATATACACTG ACTGCCAGGG CCGAAGATCT 
AGACAACAGG AATTGATGTG GCCAGCAAAT CCTTCCTCTT GTTGTTACAA TGCACCATAT 
CTCTCGGTGT ACAGTGAAAA TGCAGTTGAT ATCTTTGATG TGAACTCCAT GGAATGGATT 
CAGACTCTTC CTCTCAAAAA GGTTCGACCC TTAAACAATG AAGGATCATT AAATCTTTTA 
GGGTTGGAGA CCATTAGATT AATATATTTC AAAAATAAGA TGGCAGAAGG GGACGAACTG 
GTAGTACCTG AAACATCAGA TAATAGTCGG AAACAAATGG TTAGAAACAT TAACAATAAG 
CGGCGTTATT CCTTCAGAGT CCCAGAAGAG GAAAGGATGC AGCAGAGGAG GGAAATGCTA 
CGAGATCCAG AAATGAGAAA TAAATTAATT TCTAATCCAA CTAATTTTAA TCACATAGCA 
CACATGGGTC CTGGAGATGG AATACAGATC CTGAAAGATC TGCCCATGAA CCCTCGGCCT 
CAGGAAAGTC GGACAGTATT CAGTGGCTCA GTCAGTATTC CATCTATCAC CAAATCCCGC 
CCTGAGCCAG GCCGCTCCAT GAGTGCTAGC AGTGGCTTGT CAGCAAGGTC ATCCGCACAG 
AATGGCAGCG CATTAAAGAG GGAATTCTCT GGAGGAAGCT ACAGTGCCAA GCGGCAGCCC 
ATGCCCTCCC CGTCAGAGGG CTCTTTGTCC TCTGGAGGCA TGGACCAAGG AAGTGATGCC 
CCAGCGAGGG ACTTTGACGG AGAGGACTCT GACTCTCCGA GGCATTCCAC AGCTTCCAAC 
AGTTCCAACC TAAGCAGCCC CCCAAGCCCA GCTTCACCCC GAAAAACCAA GAGCCTCTCC 
CTGGAGAGCA CTGACCGCGG GAGCTGGGAC CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352939.1
CDS	1311..6386
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	NM_001366010.1
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001366010.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCAAG ACTCACCAGT TTTTTGTAAA ATCTTTTACT 
ACTCCTACCA AGTGTCATCA GTGTACCTCC TTGATGGTGG GTTTAATAAG ACAGGGCTGT 
TCATGTGAAG TGTGTGGATT CTCATGCCAT ATAACTTGTG TAAACAAAGC TCCAACCACT 
TGTCCAGTTC CTCCTGAACA GACAAAAGGT CCCCTGGGTA TAGATCCTCA GAAAGGAATA 
GGAACAGCAT ATGAAGGTCA TGTCAGGATT CCTAAGCCAG CTGGAGTGAA GAAAGGGTGG 
CAGAGAGCAC TGGCTATAGT GTGTGACTTC AAACTCTTTC TGTACGATAT TGCTGAAGGA 
AAAGCATCTC AGCCCAGTGT TGTCATTAGT CAAGTGATTG ACATGAGGGA TGAAGAATTT 
TCTGTGAGTT CAGTCTTGGC TTCTGATGTT ATCCATGCAA GTCGGAAAGA TATACCCTGT 
ATATTTAGGG TCACAGCTTC CCAGCTCTCA GCATCTAATA ACAAATGTTC AATCCTGATG 
CTAGCAGACA CTGAGAATGA GAAGAATAAG TGGGTGGGAG TGCTGAGTGA ATTGCACAAG 
ATTTTGAAGA AAAACAAATT CAGAGACCGC TCAGTCTATG TTCCCAAAGA GGCTTATGAC 
AGCACTCTAC CCCTCATTAA AACAACCCAG GCAGCCGCAA TCATAGATCA TGAAAGAATT 
GCTTTGGGAA ACGAAGAAGG GTTATTTGTT GTACATGTCA CCAAAGATGA AATTATTAGA 
GTTGGTGACA ATAAGAAGAT TCATCAGATT GAACTCATTC CAAATGATCA GCTTGTTGCT 
GTGATCTCAG GACGAAATCG TCATGTACGA CTTTTTCCTA TGTCAGCATT GGATGGGCGA 
GAGACCGATT TTTACAAGCT GTCAGAAACT AAAGGGTGTC AAACCGTAAC TTCTGGAAAG 
GTGCGCCATG GAGCTCTCAC ATGCCTGTGT GTGGCTATGA AAAGGCAGGT CCTCTGTTAT 
GAACTATTTC AGAGCAAGAC CCGTCACAGA AAATTTAAAG AAATTCAAGT CCCATATAAT 
GTCCAGTGGA TGGCAATCTT CAGTGAACAA CTCTGTGTGG GATTCCAGTC AGGATTTCTA 
AGATACCCCT TGAATGGAGA AGGAAATCCA TACAGTATGC TCCATTCAAA TGACCATACA 
CTATCATTTA TTGCACATCA ACCAATGGAT GCTATCTGCG CAGTTGAGAT CTCCAGTAAA 
GAATATCTGC TGTGTTTTAA CAGCATTGGG ATATACACTG ACTGCCAGGG CCGAAGATCT 
AGACAACAGG AATTGATGTG GCCAGCAAAT CCTTCCTCTT GTTGTTACAA TGCACCATAT 
CTCTCGGTGT ACAGTGAAAA TGCAGTTGAT ATCTTTGATG TGAACTCCAT GGAATGGATT 
CAGACTCTTC CTCTCAAAAA GGTTCGACCC TTAAACAATG AAGGATCATT AAATCTTTTA 
GGGTTGGAGA CCATTAGATT AATATATTTC AAAAATAAGA TGGCAGAAGG GGACGAACTG 
GTAGTACCTG AAACATCAGA TAATAGTCGG AAACAAATGG TTAGAAACAT TAACAATAAG 
CGGCGTTATT CCTTCAGAGT CCCAGAAGAG GAAAGGATGC AGCAGAGGAG GGAAATGCTA 
CGAGATCCAG AAATGAGAAA TAAATTAATT TCTAATCCAA CTAATTTTAA TCACATAGCA 
CACATGGGTC CTGGAGATGG AATACAGATC CTGAAAGATC TGCCCATGAA CCCTCGGCCT 
CAGGAAAGTC GGACAGTATT CAGTGGCTCA GTCAGTATTC CATCTATCAC CAAATCCCGC 
CCTGAGCCAG GCCGCTCCAT GAGTGCTAGC AGTGGCTTGT CAGCAAGGTC ATCCGCACAG 
AATGGCAGCG CATTAAAGAG GGAATTCTCT GGAGGAAGCT ACAGTGCCAA GCGGCAGCCC 
ATGCCCTCCC CGTCAGAGGG CTCTTTGTCC TCTGGAGGCA TGGACCAAGG AAGTGATGCC 
CCAGCGAGGG ACTTTGACGG AGAGGACTCT GACTCTCCGA GGCATTCCAC AGCTTCCAAC 
AGTTCCAACC TAAGCAGCCC CCCAAGCCCA GCTTCACCCC GAAAAACCAA GAGCCTCTCC 
CTGGAGAGCA CTGACCGCGG GAGCTGGGAC CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( NM_001366010.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001352939.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant D, mRNA.

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CloneID	OHu107134
Clone ID Related Accession (Same CDS sequence)	NM_001366010.1 , NM_001366010.2
Accession Version	NM_001366010.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL627308.20, AB208968.1, BC136333.1 and AL353689.26. On Jun 2, 2019 this sequence version replaced NM_001366010.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB208968.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCAAG ACTCACCAGT TTTTTGTAAA ATCTTTTACT 
ACTCCTACCA AGTGTCATCA GTGTACCTCC TTGATGGTGG GTTTAATAAG ACAGGGCTGT 
TCATGTGAAG TGTGTGGATT CTCATGCCAT ATAACTTGTG TAAACAAAGC TCCAACCACT 
TGTCCAGTTC CTCCTGAACA GACAAAAGGT CCCCTGGGTA TAGATCCTCA GAAAGGAATA 
GGAACAGCAT ATGAAGGTCA TGTCAGGATT CCTAAGCCAG CTGGAGTGAA GAAAGGGTGG 
CAGAGAGCAC TGGCTATAGT GTGTGACTTC AAACTCTTTC TGTACGATAT TGCTGAAGGA 
AAAGCATCTC AGCCCAGTGT TGTCATTAGT CAAGTGATTG ACATGAGGGA TGAAGAATTT 
TCTGTGAGTT CAGTCTTGGC TTCTGATGTT ATCCATGCAA GTCGGAAAGA TATACCCTGT 
ATATTTAGGG TCACAGCTTC CCAGCTCTCA GCATCTAATA ACAAATGTTC AATCCTGATG 
CTAGCAGACA CTGAGAATGA GAAGAATAAG TGGGTGGGAG TGCTGAGTGA ATTGCACAAG 
ATTTTGAAGA AAAACAAATT CAGAGACCGC TCAGTCTATG TTCCCAAAGA GGCTTATGAC 
AGCACTCTAC CCCTCATTAA AACAACCCAG GCAGCCGCAA TCATAGATCA TGAAAGAATT 
GCTTTGGGAA ACGAAGAAGG GTTATTTGTT GTACATGTCA CCAAAGATGA AATTATTAGA 
GTTGGTGACA ATAAGAAGAT TCATCAGATT GAACTCATTC CAAATGATCA GCTTGTTGCT 
GTGATCTCAG GACGAAATCG TCATGTACGA CTTTTTCCTA TGTCAGCATT GGATGGGCGA 
GAGACCGATT TTTACAAGCT GTCAGAAACT AAAGGGTGTC AAACCGTAAC TTCTGGAAAG 
GTGCGCCATG GAGCTCTCAC ATGCCTGTGT GTGGCTATGA AAAGGCAGGT CCTCTGTTAT 
GAACTATTTC AGAGCAAGAC CCGTCACAGA AAATTTAAAG AAATTCAAGT CCCATATAAT 
GTCCAGTGGA TGGCAATCTT CAGTGAACAA CTCTGTGTGG GATTCCAGTC AGGATTTCTA 
AGATACCCCT TGAATGGAGA AGGAAATCCA TACAGTATGC TCCATTCAAA TGACCATACA 
CTATCATTTA TTGCACATCA ACCAATGGAT GCTATCTGCG CAGTTGAGAT CTCCAGTAAA 
GAATATCTGC TGTGTTTTAA CAGCATTGGG ATATACACTG ACTGCCAGGG CCGAAGATCT 
AGACAACAGG AATTGATGTG GCCAGCAAAT CCTTCCTCTT GTTGTTACAA TGCACCATAT 
CTCTCGGTGT ACAGTGAAAA TGCAGTTGAT ATCTTTGATG TGAACTCCAT GGAATGGATT 
CAGACTCTTC CTCTCAAAAA GGTTCGACCC TTAAACAATG AAGGATCATT AAATCTTTTA 
GGGTTGGAGA CCATTAGATT AATATATTTC AAAAATAAGA TGGCAGAAGG GGACGAACTG 
GTAGTACCTG AAACATCAGA TAATAGTCGG AAACAAATGG TTAGAAACAT TAACAATAAG 
CGGCGTTATT CCTTCAGAGT CCCAGAAGAG GAAAGGATGC AGCAGAGGAG GGAAATGCTA 
CGAGATCCAG AAATGAGAAA TAAATTAATT TCTAATCCAA CTAATTTTAA TCACATAGCA 
CACATGGGTC CTGGAGATGG AATACAGATC CTGAAAGATC TGCCCATGAA CCCTCGGCCT 
CAGGAAAGTC GGACAGTATT CAGTGGCTCA GTCAGTATTC CATCTATCAC CAAATCCCGC 
CCTGAGCCAG GCCGCTCCAT GAGTGCTAGC AGTGGCTTGT CAGCAAGGTC ATCCGCACAG 
AATGGCAGCG CATTAAAGAG GGAATTCTCT GGAGGAAGCT ACAGTGCCAA GCGGCAGCCC 
ATGCCCTCCC CGTCAGAGGG CTCTTTGTCC TCTGGAGGCA TGGACCAAGG AAGTGATGCC 
CCAGCGAGGG ACTTTGACGG AGAGGACTCT GACTCTCCGA GGCATTCCAC AGCTTCCAAC 
AGTTCCAACC TAAGCAGCCC CCCAAGCCCA GCTTCACCCC GAAAAACCAA GAGCCTCTCC 
CTGGAGAGCA CTGACCGCGG GAGCTGGGAC CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352939.1
CDS	1311..6386
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	NM_001366010.2
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001366010.2

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCAAG ACTCACCAGT TTTTTGTAAA ATCTTTTACT 
ACTCCTACCA AGTGTCATCA GTGTACCTCC TTGATGGTGG GTTTAATAAG ACAGGGCTGT 
TCATGTGAAG TGTGTGGATT CTCATGCCAT ATAACTTGTG TAAACAAAGC TCCAACCACT 
TGTCCAGTTC CTCCTGAACA GACAAAAGGT CCCCTGGGTA TAGATCCTCA GAAAGGAATA 
GGAACAGCAT ATGAAGGTCA TGTCAGGATT CCTAAGCCAG CTGGAGTGAA GAAAGGGTGG 
CAGAGAGCAC TGGCTATAGT GTGTGACTTC AAACTCTTTC TGTACGATAT TGCTGAAGGA 
AAAGCATCTC AGCCCAGTGT TGTCATTAGT CAAGTGATTG ACATGAGGGA TGAAGAATTT 
TCTGTGAGTT CAGTCTTGGC TTCTGATGTT ATCCATGCAA GTCGGAAAGA TATACCCTGT 
ATATTTAGGG TCACAGCTTC CCAGCTCTCA GCATCTAATA ACAAATGTTC AATCCTGATG 
CTAGCAGACA CTGAGAATGA GAAGAATAAG TGGGTGGGAG TGCTGAGTGA ATTGCACAAG 
ATTTTGAAGA AAAACAAATT CAGAGACCGC TCAGTCTATG TTCCCAAAGA GGCTTATGAC 
AGCACTCTAC CCCTCATTAA AACAACCCAG GCAGCCGCAA TCATAGATCA TGAAAGAATT 
GCTTTGGGAA ACGAAGAAGG GTTATTTGTT GTACATGTCA CCAAAGATGA AATTATTAGA 
GTTGGTGACA ATAAGAAGAT TCATCAGATT GAACTCATTC CAAATGATCA GCTTGTTGCT 
GTGATCTCAG GACGAAATCG TCATGTACGA CTTTTTCCTA TGTCAGCATT GGATGGGCGA 
GAGACCGATT TTTACAAGCT GTCAGAAACT AAAGGGTGTC AAACCGTAAC TTCTGGAAAG 
GTGCGCCATG GAGCTCTCAC ATGCCTGTGT GTGGCTATGA AAAGGCAGGT CCTCTGTTAT 
GAACTATTTC AGAGCAAGAC CCGTCACAGA AAATTTAAAG AAATTCAAGT CCCATATAAT 
GTCCAGTGGA TGGCAATCTT CAGTGAACAA CTCTGTGTGG GATTCCAGTC AGGATTTCTA 
AGATACCCCT TGAATGGAGA AGGAAATCCA TACAGTATGC TCCATTCAAA TGACCATACA 
CTATCATTTA TTGCACATCA ACCAATGGAT GCTATCTGCG CAGTTGAGAT CTCCAGTAAA 
GAATATCTGC TGTGTTTTAA CAGCATTGGG ATATACACTG ACTGCCAGGG CCGAAGATCT 
AGACAACAGG AATTGATGTG GCCAGCAAAT CCTTCCTCTT GTTGTTACAA TGCACCATAT 
CTCTCGGTGT ACAGTGAAAA TGCAGTTGAT ATCTTTGATG TGAACTCCAT GGAATGGATT 
CAGACTCTTC CTCTCAAAAA GGTTCGACCC TTAAACAATG AAGGATCATT AAATCTTTTA 
GGGTTGGAGA CCATTAGATT AATATATTTC AAAAATAAGA TGGCAGAAGG GGACGAACTG 
GTAGTACCTG AAACATCAGA TAATAGTCGG AAACAAATGG TTAGAAACAT TAACAATAAG 
CGGCGTTATT CCTTCAGAGT CCCAGAAGAG GAAAGGATGC AGCAGAGGAG GGAAATGCTA 
CGAGATCCAG AAATGAGAAA TAAATTAATT TCTAATCCAA CTAATTTTAA TCACATAGCA 
CACATGGGTC CTGGAGATGG AATACAGATC CTGAAAGATC TGCCCATGAA CCCTCGGCCT 
CAGGAAAGTC GGACAGTATT CAGTGGCTCA GTCAGTATTC CATCTATCAC CAAATCCCGC 
CCTGAGCCAG GCCGCTCCAT GAGTGCTAGC AGTGGCTTGT CAGCAAGGTC ATCCGCACAG 
AATGGCAGCG CATTAAAGAG GGAATTCTCT GGAGGAAGCT ACAGTGCCAA GCGGCAGCCC 
ATGCCCTCCC CGTCAGAGGG CTCTTTGTCC TCTGGAGGCA TGGACCAAGG AAGTGATGCC 
CCAGCGAGGG ACTTTGACGG AGAGGACTCT GACTCTCCGA GGCATTCCAC AGCTTCCAAC 
AGTTCCAACC TAAGCAGCCC CCCAAGCCCA GCTTCACCCC GAAAAACCAA GAGCCTCTCC 
CTGGAGAGCA CTGACCGCGG GAGCTGGGAC CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( NM_001366011.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001352940.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant E, mRNA.

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CloneID	OHu107744
Clone ID Related Accession (Same CDS sequence)	NM_001366011.1
Accession Version	NM_001366011.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL627308.20, BC136333.1 and AL353689.26. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC136333.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGCTA GCAAAGGCAG ACGTACTGTA GACTCCACTC CACTTTCAGT TCACACACCA 
ACCTTAAGGA AAAAAGGATG TCCTGGTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAC 
CAGTTTTTTG TAAAATCTTT TACTACTCCT ACCAAGTGTC ATCAGTGTAC CTCCTTGATG 
GTGGGTTTAA TAAGACAGGG CTGTTCATGT GAAGTGTGTG GATTCTCATG CCATATAACT 
TGTGTAAACA AAGCTCCAAC CACTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCCCTG 
GGTATAGATC CTCAGAAAGG AATAGGAACA GCATATGAAG GTCATGTCAG GATTCCTAAG 
CCAGCTGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTA TAGTGTGTGA CTTCAAACTC 
TTTCTGTACG ATATTGCTGA AGGAAAAGCA TCTCAGCCCA GTGTTGTCAT TAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATCCAT 
GCAAGTCGGA AAGATATACC CTGTATATTT AGGGTCACAG CTTCCCAGCT CTCAGCATCT 
AATAACAAAT GTTCAATCCT GATGCTAGCA GACACTGAGA ATGAGAAGAA TAAGTGGGTG 
GGAGTGCTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTACCCCTCA TTAAAACAAC CCAGGCAGCC 
GCAATCATAG ATCATGAAAG AATTGCTTTG GGAAACGAAG AAGGGTTATT TGTTGTACAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATGTCAG CATTGGATGG GCGAGAGACC GATTTTTACA AGCTGTCAGA AACTAAAGGG 
TGTCAAACCG TAACTTCTGG AAAGGTGCGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGAAAATTT 
AAAGAAATTC AAGTCCCATA TAATGTCCAG TGGATGGCAA TCTTCAGTGA ACAACTCTGT 
GTGGGATTCC AGTCAGGATT TCTAAGATAC CCCTTGAATG GAGAAGGAAA TCCATACAGT 
ATGCTCCATT CAAATGACCA TACACTATCA TTTATTGCAC ATCAACCAAT GGATGCTATC 
TGCGCAGTTG AGATCTCCAG TAAAGAATAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAATTGA TGTGGCCAGC AAATCCTTCC 
TCTTGTTGTT ACAATGCACC ATATCTCTCG GTGTACAGTG AAAATGCAGT TGATATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTTCCTCTCA AAAAGGTTCG ACCCTTAAAC 
AATGAAGGAT CATTAAATCT TTTAGGGTTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGACGA ACTGGTAGTA CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ACATTAACAA TAAGCGGCGT TATTCCTTCA GAGTCCCAGA AGAGGAAAGG 
ATGCAGCAGA GGAGGGAAAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAT 
CCAACTAATT TTAATCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTGAAA 
GATCTGCCCA TGAACCCTCG GCCTCAGGAA AGTCGGACAG TATTCAGTGG CTCAGTCAGT 
ATTCCATCTA TCACCAAATC CCGCCCTGAG CCAGGCCGCT CCATGAGTGC TAGCAGTGGC 
TTGTCAGCAA GGTCATCCGC ACAGAATGGC AGCGCATTAA AGAGGGAATT CTCTGGAGGA 
AGCTACAGTG CCAAGCGGCA GCCCATGCCC TCCCCGTCAG AGGGCTCTTT GTCCTCTGGA 
GGCATGGACC AAGGAAGTGA TGCCCCAGCG AGGGACTTTG ACGGAGAGGA CTCTGACTCT 
CCGAGGCATT CCACAGCTTC CAACAGTTCC AACCTAAGCA GCCCCCCAAG CCCAGCTTCA 
CCCCGAAAAA CCAAGAGCCT CTCCCTGGAG AGCACTGACC GCGGGAGCTG GGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352940.1
CDS	1311..6410
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKASKGRRTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	NM_001366011.1
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001366011.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGCTA GCAAAGGCAG ACGTACTGTA GACTCCACTC CACTTTCAGT TCACACACCA 
ACCTTAAGGA AAAAAGGATG TCCTGGTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAC 
CAGTTTTTTG TAAAATCTTT TACTACTCCT ACCAAGTGTC ATCAGTGTAC CTCCTTGATG 
GTGGGTTTAA TAAGACAGGG CTGTTCATGT GAAGTGTGTG GATTCTCATG CCATATAACT 
TGTGTAAACA AAGCTCCAAC CACTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCCCTG 
GGTATAGATC CTCAGAAAGG AATAGGAACA GCATATGAAG GTCATGTCAG GATTCCTAAG 
CCAGCTGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTA TAGTGTGTGA CTTCAAACTC 
TTTCTGTACG ATATTGCTGA AGGAAAAGCA TCTCAGCCCA GTGTTGTCAT TAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATCCAT 
GCAAGTCGGA AAGATATACC CTGTATATTT AGGGTCACAG CTTCCCAGCT CTCAGCATCT 
AATAACAAAT GTTCAATCCT GATGCTAGCA GACACTGAGA ATGAGAAGAA TAAGTGGGTG 
GGAGTGCTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTACCCCTCA TTAAAACAAC CCAGGCAGCC 
GCAATCATAG ATCATGAAAG AATTGCTTTG GGAAACGAAG AAGGGTTATT TGTTGTACAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATGTCAG CATTGGATGG GCGAGAGACC GATTTTTACA AGCTGTCAGA AACTAAAGGG 
TGTCAAACCG TAACTTCTGG AAAGGTGCGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGAAAATTT 
AAAGAAATTC AAGTCCCATA TAATGTCCAG TGGATGGCAA TCTTCAGTGA ACAACTCTGT 
GTGGGATTCC AGTCAGGATT TCTAAGATAC CCCTTGAATG GAGAAGGAAA TCCATACAGT 
ATGCTCCATT CAAATGACCA TACACTATCA TTTATTGCAC ATCAACCAAT GGATGCTATC 
TGCGCAGTTG AGATCTCCAG TAAAGAATAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAATTGA TGTGGCCAGC AAATCCTTCC 
TCTTGTTGTT ACAATGCACC ATATCTCTCG GTGTACAGTG AAAATGCAGT TGATATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTTCCTCTCA AAAAGGTTCG ACCCTTAAAC 
AATGAAGGAT CATTAAATCT TTTAGGGTTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGACGA ACTGGTAGTA CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ACATTAACAA TAAGCGGCGT TATTCCTTCA GAGTCCCAGA AGAGGAAAGG 
ATGCAGCAGA GGAGGGAAAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAT 
CCAACTAATT TTAATCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTGAAA 
GATCTGCCCA TGAACCCTCG GCCTCAGGAA AGTCGGACAG TATTCAGTGG CTCAGTCAGT 
ATTCCATCTA TCACCAAATC CCGCCCTGAG CCAGGCCGCT CCATGAGTGC TAGCAGTGGC 
TTGTCAGCAA GGTCATCCGC ACAGAATGGC AGCGCATTAA AGAGGGAATT CTCTGGAGGA 
AGCTACAGTG CCAAGCGGCA GCCCATGCCC TCCCCGTCAG AGGGCTCTTT GTCCTCTGGA 
GGCATGGACC AAGGAAGTGA TGCCCCAGCG AGGGACTTTG ACGGAGAGGA CTCTGACTCT 
CCGAGGCATT CCACAGCTTC CAACAGTTCC AACCTAAGCA GCCCCCCAAG CCCAGCTTCA 
CCCCGAAAAA CCAAGAGCCT CTCCCTGGAG AGCACTGACC GCGGGAGCTG GGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_017002578.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858067.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X10, mRNA.

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CloneID	OHu90668
Clone ID Related Accession (Same CDS sequence)	XM_017002578.1 , XM_017002578.2
Accession Version	XM_017002578.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGATC CCGTTGAGAA CACATATGTA TGGAACCCGA GCGTCAAGTT TCACATCCAG 
TCACGGTCCA CATCTCCTTC CACATCTAGT GAAGCTGAGC CAGTTAAGAC TGTAGACTCC 
ACTCCACTTT CAGTTCACAC ACCAACCTTA AGGAAAAAAG GATGTCCTGG TTCAACTGGC 
TTTCCACCTA AGCGCAAGAC TCACCAGTTT TTTGTAAAAT CTTTTACTAC TCCTACCAAG 
TGTCATCAGT GTACCTCCTT GATGGTGGGT TTAATAAGAC AGGGCTGTTC ATGTGAAGTG 
TGTGGATTCT CATGCCATAT AACTTGTGTA AACAAAGCTC CAACCACTTG TCCAGTTCCT 
CCTGAACAGA CAAAAGGTCC CCTGGGTATA GATCCTCAGA AAGGAATAGG AACAGCATAT 
GAAGGTCATG TCAGGATTCC TAAGCCAGCT GGAGTGAAGA AAGGGTGGCA GAGAGCACTG 
GCTATAGTGT GTGACTTCAA ACTCTTTCTG TACGATATTG CTGAAGGAAA AGCATCTCAG 
CCCAGTGTTG TCATTAGTCA AGTGATTGAC ATGAGGGATG AAGAATTTTC TGTGAGTTCA 
GTCTTGGCTT CTGATGTTAT CCATGCAAGT CGGAAAGATA TACCCTGTAT ATTTAGGGTC 
ACAGCTTCCC AGCTCTCAGC ATCTAATAAC AAATGTTCAA TCCTGATGCT AGCAGACACT 
GAGAATGAGA AGAATAAGTG GGTGGGAGTG CTGAGTGAAT TGCACAAGAT TTTGAAGAAA 
AACAAATTCA GAGACCGCTC AGTCTATGTT CCCAAAGAGG CTTATGACAG CACTCTACCC 
CTCATTAAAA CAACCCAGGC AGCCGCAATC ATAGATCATG AAAGAATTGC TTTGGGAAAC 
GAAGAAGGGT TATTTGTTGT ACATGTCACC AAAGATGAAA TTATTAGAGT TGGTGACAAT 
AAGAAGATTC ATCAGATTGA ACTCATTCCA AATGATCAGC TTGTTGCTGT GATCTCAGGA 
CGAAATCGTC ATGTACGACT TTTTCCTATG TCAGCATTGG ATGGGCGAGA GACCGATTTT 
TACAAGCTGT CAGAAACTAA AGGGTGTCAA ACCGTAACTT CTGGAAAGGT GCGCCATGGA 
GCTCTCACAT GCCTGTGTGT GGCTATGAAA AGGCAGGTCC TCTGTTATGA ACTATTTCAG 
AGCAAGACCC GTCACAGAAA ATTTAAAGAA ATTCAAGTCC CATATAATGT CCAGTGGATG 
GCAATCTTCA GTGAACAACT CTGTGTGGGA TTCCAGTCAG GATTTCTAAG ATACCCCTTG 
AATGGAGAAG GAAATCCATA CAGTATGCTC CATTCAAATG ACCATACACT ATCATTTATT 
GCACATCAAC CAATGGATGC TATCTGCGCA GTTGAGATCT CCAGTAAAGA ATATCTGCTG 
TGTTTTAACA GCATTGGGAT ATACACTGAC TGCCAGGGCC GAAGATCTAG ACAACAGGAA 
TTGATGTGGC CAGCAAATCC TTCCTCTTGT TGTTACAATG CACCATATCT CTCGGTGTAC 
AGTGAAAATG CAGTTGATAT CTTTGATGTG AACTCCATGG AATGGATTCA GACTCTTCCT 
CTCAAAAAGG TTCGACCCTT AAACAATGAA GGATCATTAA ATCTTTTAGG GTTGGAGACC 
ATTAGATTAA TATATTTCAA AAATAAGATG GCAGAAGGGG ACGAACTGGT AGTACCTGAA 
ACATCAGATA ATAGTCGGAA ACAAATGGTT AGAAACATTA ACAATAAGCG GCGTTATTCC 
TTCAGAGTCC CAGAAGAGGA AAGGATGCAG CAGAGGAGGG AAATGCTACG AGATCCAGAA 
ATGAGAAATA AATTAATTTC TAATCCAACT AATTTTAATC ACATAGCACA CATGGGTCCT 
GGAGATGGAA TACAGATCCT GAAAGATCTG CCCATGAACC CTCGGCCTCA GGAAAGTCGG 
ACAGTATTCA GTGGCTCAGT CAGTATTCCA TCTATCACCA AATCCCGCCC TGAGCCAGGC 
CGCTCCATGA GTGCTAGCAG TGGCTTGTCA GCAAGGTCAT CCGCACAGAA TGGCAGCGCA 
TTAAAGAGGG AATTCTCTGG AGGAAGCTAC AGTGCCAAGC GGCAGCCCAT GCCCTCCCCG 
TCAGAGGGCT CTTTGTCCTC TGGAGGCATG GACCAAGGAA GTGATGCCCC AGCGAGGGAC 
TTTGACGGAG AGGACTCTGA CTCTCCGAGG CATTCCACAG CTTCCAACAG TTCCAACCTA 
AGCAGCCCCC CAAGCCCAGC TTCACCCCGA AAAACCAAGA GCCTCTCCCT GGAGAGCACT 
GACCGCGGGA GCTGGGACCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858067.1
CDS	1056..6359
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRTDPVENTYVWNPSVKFHIQSRSTSPSTSSEAEPVKTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_017002578.1
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002578.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGATC CCGTTGAGAA CACATATGTA TGGAACCCGA GCGTCAAGTT TCACATCCAG 
TCACGGTCCA CATCTCCTTC CACATCTAGT GAAGCTGAGC CAGTTAAGAC TGTAGACTCC 
ACTCCACTTT CAGTTCACAC ACCAACCTTA AGGAAAAAAG GATGTCCTGG TTCAACTGGC 
TTTCCACCTA AGCGCAAGAC TCACCAGTTT TTTGTAAAAT CTTTTACTAC TCCTACCAAG 
TGTCATCAGT GTACCTCCTT GATGGTGGGT TTAATAAGAC AGGGCTGTTC ATGTGAAGTG 
TGTGGATTCT CATGCCATAT AACTTGTGTA AACAAAGCTC CAACCACTTG TCCAGTTCCT 
CCTGAACAGA CAAAAGGTCC CCTGGGTATA GATCCTCAGA AAGGAATAGG AACAGCATAT 
GAAGGTCATG TCAGGATTCC TAAGCCAGCT GGAGTGAAGA AAGGGTGGCA GAGAGCACTG 
GCTATAGTGT GTGACTTCAA ACTCTTTCTG TACGATATTG CTGAAGGAAA AGCATCTCAG 
CCCAGTGTTG TCATTAGTCA AGTGATTGAC ATGAGGGATG AAGAATTTTC TGTGAGTTCA 
GTCTTGGCTT CTGATGTTAT CCATGCAAGT CGGAAAGATA TACCCTGTAT ATTTAGGGTC 
ACAGCTTCCC AGCTCTCAGC ATCTAATAAC AAATGTTCAA TCCTGATGCT AGCAGACACT 
GAGAATGAGA AGAATAAGTG GGTGGGAGTG CTGAGTGAAT TGCACAAGAT TTTGAAGAAA 
AACAAATTCA GAGACCGCTC AGTCTATGTT CCCAAAGAGG CTTATGACAG CACTCTACCC 
CTCATTAAAA CAACCCAGGC AGCCGCAATC ATAGATCATG AAAGAATTGC TTTGGGAAAC 
GAAGAAGGGT TATTTGTTGT ACATGTCACC AAAGATGAAA TTATTAGAGT TGGTGACAAT 
AAGAAGATTC ATCAGATTGA ACTCATTCCA AATGATCAGC TTGTTGCTGT GATCTCAGGA 
CGAAATCGTC ATGTACGACT TTTTCCTATG TCAGCATTGG ATGGGCGAGA GACCGATTTT 
TACAAGCTGT CAGAAACTAA AGGGTGTCAA ACCGTAACTT CTGGAAAGGT GCGCCATGGA 
GCTCTCACAT GCCTGTGTGT GGCTATGAAA AGGCAGGTCC TCTGTTATGA ACTATTTCAG 
AGCAAGACCC GTCACAGAAA ATTTAAAGAA ATTCAAGTCC CATATAATGT CCAGTGGATG 
GCAATCTTCA GTGAACAACT CTGTGTGGGA TTCCAGTCAG GATTTCTAAG ATACCCCTTG 
AATGGAGAAG GAAATCCATA CAGTATGCTC CATTCAAATG ACCATACACT ATCATTTATT 
GCACATCAAC CAATGGATGC TATCTGCGCA GTTGAGATCT CCAGTAAAGA ATATCTGCTG 
TGTTTTAACA GCATTGGGAT ATACACTGAC TGCCAGGGCC GAAGATCTAG ACAACAGGAA 
TTGATGTGGC CAGCAAATCC TTCCTCTTGT TGTTACAATG CACCATATCT CTCGGTGTAC 
AGTGAAAATG CAGTTGATAT CTTTGATGTG AACTCCATGG AATGGATTCA GACTCTTCCT 
CTCAAAAAGG TTCGACCCTT AAACAATGAA GGATCATTAA ATCTTTTAGG GTTGGAGACC 
ATTAGATTAA TATATTTCAA AAATAAGATG GCAGAAGGGG ACGAACTGGT AGTACCTGAA 
ACATCAGATA ATAGTCGGAA ACAAATGGTT AGAAACATTA ACAATAAGCG GCGTTATTCC 
TTCAGAGTCC CAGAAGAGGA AAGGATGCAG CAGAGGAGGG AAATGCTACG AGATCCAGAA 
ATGAGAAATA AATTAATTTC TAATCCAACT AATTTTAATC ACATAGCACA CATGGGTCCT 
GGAGATGGAA TACAGATCCT GAAAGATCTG CCCATGAACC CTCGGCCTCA GGAAAGTCGG 
ACAGTATTCA GTGGCTCAGT CAGTATTCCA TCTATCACCA AATCCCGCCC TGAGCCAGGC 
CGCTCCATGA GTGCTAGCAG TGGCTTGTCA GCAAGGTCAT CCGCACAGAA TGGCAGCGCA 
TTAAAGAGGG AATTCTCTGG AGGAAGCTAC AGTGCCAAGC GGCAGCCCAT GCCCTCCCCG 
TCAGAGGGCT CTTTGTCCTC TGGAGGCATG GACCAAGGAA GTGATGCCCC AGCGAGGGAC 
TTTGACGGAG AGGACTCTGA CTCTCCGAGG CATTCCACAG CTTCCAACAG TTCCAACCTA 
AGCAGCCCCC CAAGCCCAGC TTCACCCCGA AAAACCAAGA GCCTCTCCCT GGAGAGCACT 
GACCGCGGGA GCTGGGACCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_017002578.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858067.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X10, mRNA.

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CloneID	OHu90668
Clone ID Related Accession (Same CDS sequence)	XM_017002578.1 , XM_017002578.2
Accession Version	XM_017002578.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017002578.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGATC CCGTTGAGAA CACATATGTA TGGAACCCGA GCGTCAAGTT TCACATCCAG 
TCACGGTCCA CATCTCCTTC CACATCTAGT GAAGCTGAGC CAGTTAAGAC TGTAGACTCC 
ACTCCACTTT CAGTTCACAC ACCAACCTTA AGGAAAAAAG GATGTCCTGG TTCAACTGGC 
TTTCCACCTA AGCGCAAGAC TCACCAGTTT TTTGTAAAAT CTTTTACTAC TCCTACCAAG 
TGTCATCAGT GTACCTCCTT GATGGTGGGT TTAATAAGAC AGGGCTGTTC ATGTGAAGTG 
TGTGGATTCT CATGCCATAT AACTTGTGTA AACAAAGCTC CAACCACTTG TCCAGTTCCT 
CCTGAACAGA CAAAAGGTCC CCTGGGTATA GATCCTCAGA AAGGAATAGG AACAGCATAT 
GAAGGTCATG TCAGGATTCC TAAGCCAGCT GGAGTGAAGA AAGGGTGGCA GAGAGCACTG 
GCTATAGTGT GTGACTTCAA ACTCTTTCTG TACGATATTG CTGAAGGAAA AGCATCTCAG 
CCCAGTGTTG TCATTAGTCA AGTGATTGAC ATGAGGGATG AAGAATTTTC TGTGAGTTCA 
GTCTTGGCTT CTGATGTTAT CCATGCAAGT CGGAAAGATA TACCCTGTAT ATTTAGGGTC 
ACAGCTTCCC AGCTCTCAGC ATCTAATAAC AAATGTTCAA TCCTGATGCT AGCAGACACT 
GAGAATGAGA AGAATAAGTG GGTGGGAGTG CTGAGTGAAT TGCACAAGAT TTTGAAGAAA 
AACAAATTCA GAGACCGCTC AGTCTATGTT CCCAAAGAGG CTTATGACAG CACTCTACCC 
CTCATTAAAA CAACCCAGGC AGCCGCAATC ATAGATCATG AAAGAATTGC TTTGGGAAAC 
GAAGAAGGGT TATTTGTTGT ACATGTCACC AAAGATGAAA TTATTAGAGT TGGTGACAAT 
AAGAAGATTC ATCAGATTGA ACTCATTCCA AATGATCAGC TTGTTGCTGT GATCTCAGGA 
CGAAATCGTC ATGTACGACT TTTTCCTATG TCAGCATTGG ATGGGCGAGA GACCGATTTT 
TACAAGCTGT CAGAAACTAA AGGGTGTCAA ACCGTAACTT CTGGAAAGGT GCGCCATGGA 
GCTCTCACAT GCCTGTGTGT GGCTATGAAA AGGCAGGTCC TCTGTTATGA ACTATTTCAG 
AGCAAGACCC GTCACAGAAA ATTTAAAGAA ATTCAAGTCC CATATAATGT CCAGTGGATG 
GCAATCTTCA GTGAACAACT CTGTGTGGGA TTCCAGTCAG GATTTCTAAG ATACCCCTTG 
AATGGAGAAG GAAATCCATA CAGTATGCTC CATTCAAATG ACCATACACT ATCATTTATT 
GCACATCAAC CAATGGATGC TATCTGCGCA GTTGAGATCT CCAGTAAAGA ATATCTGCTG 
TGTTTTAACA GCATTGGGAT ATACACTGAC TGCCAGGGCC GAAGATCTAG ACAACAGGAA 
TTGATGTGGC CAGCAAATCC TTCCTCTTGT TGTTACAATG CACCATATCT CTCGGTGTAC 
AGTGAAAATG CAGTTGATAT CTTTGATGTG AACTCCATGG AATGGATTCA GACTCTTCCT 
CTCAAAAAGG TTCGACCCTT AAACAATGAA GGATCATTAA ATCTTTTAGG GTTGGAGACC 
ATTAGATTAA TATATTTCAA AAATAAGATG GCAGAAGGGG ACGAACTGGT AGTACCTGAA 
ACATCAGATA ATAGTCGGAA ACAAATGGTT AGAAACATTA ACAATAAGCG GCGTTATTCC 
TTCAGAGTCC CAGAAGAGGA AAGGATGCAG CAGAGGAGGG AAATGCTACG AGATCCAGAA 
ATGAGAAATA AATTAATTTC TAATCCAACT AATTTTAATC ACATAGCACA CATGGGTCCT 
GGAGATGGAA TACAGATCCT GAAAGATCTG CCCATGAACC CTCGGCCTCA GGAAAGTCGG 
ACAGTATTCA GTGGCTCAGT CAGTATTCCA TCTATCACCA AATCCCGCCC TGAGCCAGGC 
CGCTCCATGA GTGCTAGCAG TGGCTTGTCA GCAAGGTCAT CCGCACAGAA TGGCAGCGCA 
TTAAAGAGGG AATTCTCTGG AGGAAGCTAC AGTGCCAAGC GGCAGCCCAT GCCCTCCCCG 
TCAGAGGGCT CTTTGTCCTC TGGAGGCATG GACCAAGGAA GTGATGCCCC AGCGAGGGAC 
TTTGACGGAG AGGACTCTGA CTCTCCGAGG CATTCCACAG CTTCCAACAG TTCCAACCTA 
AGCAGCCCCC CAAGCCCAGC TTCACCCCGA AAAACCAAGA GCCTCTCCCT GGAGAGCACT 
GACCGCGGGA GCTGGGACCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858067.1
CDS	969..6272
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRTDPVENTYVWNPSVKFHIQSRSTSPSTSSEAEPVKTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_017002578.2
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002578.2

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGATC CCGTTGAGAA CACATATGTA TGGAACCCGA GCGTCAAGTT TCACATCCAG 
TCACGGTCCA CATCTCCTTC CACATCTAGT GAAGCTGAGC CAGTTAAGAC TGTAGACTCC 
ACTCCACTTT CAGTTCACAC ACCAACCTTA AGGAAAAAAG GATGTCCTGG TTCAACTGGC 
TTTCCACCTA AGCGCAAGAC TCACCAGTTT TTTGTAAAAT CTTTTACTAC TCCTACCAAG 
TGTCATCAGT GTACCTCCTT GATGGTGGGT TTAATAAGAC AGGGCTGTTC ATGTGAAGTG 
TGTGGATTCT CATGCCATAT AACTTGTGTA AACAAAGCTC CAACCACTTG TCCAGTTCCT 
CCTGAACAGA CAAAAGGTCC CCTGGGTATA GATCCTCAGA AAGGAATAGG AACAGCATAT 
GAAGGTCATG TCAGGATTCC TAAGCCAGCT GGAGTGAAGA AAGGGTGGCA GAGAGCACTG 
GCTATAGTGT GTGACTTCAA ACTCTTTCTG TACGATATTG CTGAAGGAAA AGCATCTCAG 
CCCAGTGTTG TCATTAGTCA AGTGATTGAC ATGAGGGATG AAGAATTTTC TGTGAGTTCA 
GTCTTGGCTT CTGATGTTAT CCATGCAAGT CGGAAAGATA TACCCTGTAT ATTTAGGGTC 
ACAGCTTCCC AGCTCTCAGC ATCTAATAAC AAATGTTCAA TCCTGATGCT AGCAGACACT 
GAGAATGAGA AGAATAAGTG GGTGGGAGTG CTGAGTGAAT TGCACAAGAT TTTGAAGAAA 
AACAAATTCA GAGACCGCTC AGTCTATGTT CCCAAAGAGG CTTATGACAG CACTCTACCC 
CTCATTAAAA CAACCCAGGC AGCCGCAATC ATAGATCATG AAAGAATTGC TTTGGGAAAC 
GAAGAAGGGT TATTTGTTGT ACATGTCACC AAAGATGAAA TTATTAGAGT TGGTGACAAT 
AAGAAGATTC ATCAGATTGA ACTCATTCCA AATGATCAGC TTGTTGCTGT GATCTCAGGA 
CGAAATCGTC ATGTACGACT TTTTCCTATG TCAGCATTGG ATGGGCGAGA GACCGATTTT 
TACAAGCTGT CAGAAACTAA AGGGTGTCAA ACCGTAACTT CTGGAAAGGT GCGCCATGGA 
GCTCTCACAT GCCTGTGTGT GGCTATGAAA AGGCAGGTCC TCTGTTATGA ACTATTTCAG 
AGCAAGACCC GTCACAGAAA ATTTAAAGAA ATTCAAGTCC CATATAATGT CCAGTGGATG 
GCAATCTTCA GTGAACAACT CTGTGTGGGA TTCCAGTCAG GATTTCTAAG ATACCCCTTG 
AATGGAGAAG GAAATCCATA CAGTATGCTC CATTCAAATG ACCATACACT ATCATTTATT 
GCACATCAAC CAATGGATGC TATCTGCGCA GTTGAGATCT CCAGTAAAGA ATATCTGCTG 
TGTTTTAACA GCATTGGGAT ATACACTGAC TGCCAGGGCC GAAGATCTAG ACAACAGGAA 
TTGATGTGGC CAGCAAATCC TTCCTCTTGT TGTTACAATG CACCATATCT CTCGGTGTAC 
AGTGAAAATG CAGTTGATAT CTTTGATGTG AACTCCATGG AATGGATTCA GACTCTTCCT 
CTCAAAAAGG TTCGACCCTT AAACAATGAA GGATCATTAA ATCTTTTAGG GTTGGAGACC 
ATTAGATTAA TATATTTCAA AAATAAGATG GCAGAAGGGG ACGAACTGGT AGTACCTGAA 
ACATCAGATA ATAGTCGGAA ACAAATGGTT AGAAACATTA ACAATAAGCG GCGTTATTCC 
TTCAGAGTCC CAGAAGAGGA AAGGATGCAG CAGAGGAGGG AAATGCTACG AGATCCAGAA 
ATGAGAAATA AATTAATTTC TAATCCAACT AATTTTAATC ACATAGCACA CATGGGTCCT 
GGAGATGGAA TACAGATCCT GAAAGATCTG CCCATGAACC CTCGGCCTCA GGAAAGTCGG 
ACAGTATTCA GTGGCTCAGT CAGTATTCCA TCTATCACCA AATCCCGCCC TGAGCCAGGC 
CGCTCCATGA GTGCTAGCAG TGGCTTGTCA GCAAGGTCAT CCGCACAGAA TGGCAGCGCA 
TTAAAGAGGG AATTCTCTGG AGGAAGCTAC AGTGCCAAGC GGCAGCCCAT GCCCTCCCCG 
TCAGAGGGCT CTTTGTCCTC TGGAGGCATG GACCAAGGAA GTGATGCCCC AGCGAGGGAC 
TTTGACGGAG AGGACTCTGA CTCTCCGAGG CATTCCACAG CTTCCAACAG TTCCAACCTA 
AGCAGCCCCC CAAGCCCAGC TTCACCCCGA AAAACCAAGA GCCTCTCCCT GGAGAGCACT 
GACCGCGGGA GCTGGGACCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_017002579.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858068.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X13, mRNA.

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CloneID	OHu90669
Clone ID Related Accession (Same CDS sequence)	XM_017002579.1 , XM_017002580.2 , XM_017002580.1 , XM_017002579.2
Accession Version	XM_017002579.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATAT TGAATAAATG GGAAATGCTG AAAAGAGCTG AGACAGCATG TTTTCGTGAA 
GAAAGGGATG TATTAGTGAA TGGAGACAAT AAATGGATTA CAACCTTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTGCTTACT 
CTACTCAGCA AATTTGAAGA TAGATTGCCT GAAGATATGG CTAGATTTTA CTTGGCTGAG 
ATGGTGATAG CAATTGACTC AGTTCATCAG CTACATTATG TACACAGAGA CATTAAACCT 
GACAATATAC TGATGGATAT GAATGGACAT ATTCGGTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTTCAGTCC TCAGTGGCTG TAGGAACTCC AGATTATATC 
TCTCCTGAAA TCCTTCAAGC CATGGAAGAT GGAAAAGGGA GATATGGACC TGAATGTGAC 
TGGTGGTCTT TGGGGGTCTG TATGTATGAA ATGCTTTACG GAGAAACACC ATTTTATGCA 
GAATCGCTGG TGGAGACATA CGGAAAAATC ATGAACCACA AAGAGAGGTT TCAGTTTCCA 
GCCCAAGTGA CTGATGTGTC TGAAAATGCT AAGGATCTTA TTCGAAGGCT CATTTGTAGC 
AGAGAACATC GACTTGGTCA AAATGGAATA GAAGACTTTA AGAAACACCC ATTTTTCAGT 
GGAATTGATT GGGATAATAT TCGGAACTGT GAAGCACCTT ATATTCCAGA AGTTAGTAGC 
CCAACAGATA CATCGAATTT TGATGTAGAT GATGATTGTT TAAAAAATTC TGAAACGATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CACCATCTGC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTACTTTC TGATCGGAGC TGTTTAAGAG TTACGGCTGG TCCCACCTCA 
CTGGATCTTG ATGTTAATGT TCAGAGGACT CTAGACAACA ACTTAGCAAC TGAAGCTTAT 
GAAAGAAGAA TTAAGCGCCT TGAGCAAGAA AAACTTGAAC TCAGTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTCCAAGC TCTGCAGTAT TCAACTGTTG ATGGTCCACT AACAGCAAGC 
AAAGATTTAG AAATAAAAAA CTTAAAAGAA GAAATTGAAA AACTAAGAAA ACAAGTAACA 
GAATCAAGTC ATTTGGAACA GCAACTTGAA GAAGCTAATG CTGTGAGGCA AGAACTAGAT 
GATGCTTTTA GACAAATCAA GGCTTATGAA AAACAAATCA AAACGTTACA ACAAGAAAGA 
GAAGATCTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGACG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGGCTAACAG AATTGCACAC CCAAAAACAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGTT GAAAGCTTAA GGCAAGAACT GCGCAGAACA 
GAAAGAGCCA AAAAAGAGCT GGAAGTTCAT ACAGAAGCTC TAGCTGCTGA AGCATCTAAA 
GACAGGAAGC TACGTGAACA GAGTGAGCAC TATTCTAAGC AACTGGAAAA TGAATTGGAG 
GGACTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAGTAT GCAGCATAGA ACATCAGCAA 
GAGATAACCA AACTAAAGAC TGATTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAATTA 
TCTAAAAGAG AAGGAATACA TGCAAATGAA ATAAAAAATC TTAAGAAAGA ACTGCATGAT 
TCAGAAGGTC AGCAACTTGC TCTCAACAAA GAAATTATGA TTTTAAAAGA CAAATTGGAA 
AAAACCAGAA GAGAAAGTCA AAGTGAAAGG GAGGAATTTG AAAGTGAGTT CAAACAACAA 
TATGAACGAG AAAAAGTGTT GTTAACTGAA GAAAATAAAA AGCTGACGAG TGAACTTGAT 
AAGCTTACTA CTTTGTATGA GAACTTAAGT ATACACAACC AGCAGTTAGA AGAAGAGGTT 
AAAGATCTAG CAGACAAGAA AGAATCAGTT GCACATTGGG AAGCCCAAAT CACAGAAATA 
ATTCAGTGGG TCAGCGATGA AAAGGATGCA CGAGGGTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AATTGGAGGC ATTAAGAAAT TCCAGCTTGG GTACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCGAAACTG GATATGTCAG CTAGACTGGA GTTGCAGTCG 
GCTCTGGATG CAGAAATAAG AGCCAAACAG GCCATCCAAG AAGAGTTGAA TAAAGTTAAA 
GCATCTAATA TCATAACAGA ATGTAAACTA AAAGATTCAG AGAAGAAGAA CTTGGAACTA 
CTCTCAGAAA TCGAACAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GATCAATTTG AAGATTCCTT TTCTTCTTCC TCATCTTCAC TGATTGATTT TTTGGATGAC 
CGCTCTCCAT CCTGTACTCC AGCTAGCAAA GGCAGACGTA CTGATCCCGT TGAGAACACA 
TATGTATGGA ACCCGAGCGT CAAGTTTCAC ATCCAGTCAC GGTCCACATC TCCTTCCACA 
TCTAGTGAAG CTGAGCCAGT TAAGACTGTA GACTCCACTC CACTTTCAGT TCACACACCA 
ACCTTAAGGA AAAAAGGATG TCCTGGTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAC 
CAGTTTTTTG TAAAATCTTT TACTACTCCT ACCAAGTGTC ATCAGTGTAC CTCCTTGATG 
GTGGGTTTAA TAAGACAGGG CTGTTCATGT GAAGTGTGTG GATTCTCATG CCATATAACT 
TGTGTAAACA AAGCTCCAAC CACTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCCCTG 
GGTATAGATC CTCAGAAAGG AATAGGAACA GCATATGAAG GTCATGTCAG GATTCCTAAG 
CCAGCTGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTA TAGTGTGTGA CTTCAAACTC 
TTTCTGTACG ATATTGCTGA AGGAAAAGCA TCTCAGCCCA GTGTTGTCAT TAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATCCAT 
GCAAGTCGGA AAGATATACC CTGTATATTT AGGGTCACAG CTTCCCAGCT CTCAGCATCT 
AATAACAAAT GTTCAATCCT GATGCTAGCA GACACTGAGA ATGAGAAGAA TAAGTGGGTG 
GGAGTGCTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTACCCCTCA TTAAAACAAC CCAGGCAGCC 
GCAATCATAG ATCATGAAAG AATTGCTTTG GGAAACGAAG AAGGGTTATT TGTTGTACAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATGTCAG CATTGGATGG GCGAGAGACC GATTTTTACA AGCTGTCAGA AACTAAAGGG 
TGTCAAACCG TAACTTCTGG AAAGGTGCGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGAAAATTT 
AAAGAAATTC AAGTCCCATA TAATGTCCAG TGGATGGCAA TCTTCAGTGA ACAACTCTGT 
GTGGGATTCC AGTCAGGATT TCTAAGATAC CCCTTGAATG GAGAAGGAAA TCCATACAGT 
ATGCTCCATT CAAATGACCA TACACTATCA TTTATTGCAC ATCAACCAAT GGATGCTATC 
TGCGCAGTTG AGATCTCCAG TAAAGAATAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAATTGA TGTGGCCAGC AAATCCTTCC 
TCTTGTTGTT ACAATGCACC ATATCTCTCG GTGTACAGTG AAAATGCAGT TGATATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTTCCTCTCA AAAAGGTTCG ACCCTTAAAC 
AATGAAGGAT CATTAAATCT TTTAGGGTTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGACGA ACTGGTAGTA CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ACATTAACAA TAAGCGGCGT TATTCCTTCA GAGTCCCAGA AGAGGAAAGG 
ATGCAGCAGA GGAGGGAAAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAT 
CCAACTAATT TTAATCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTGAAA 
GATCTGCCCA TGCCAGGTTT CCCTTATCCA TCCCCTCATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAACA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGAATATTCC CCGTCTCTGC GCTCTGTCCC TGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGTC GGACAGTATT CAGTGGCTCA 
GTCAGTATTC CATCTATCAC CAAATCCCGC CCTGAGCCAG GCCGCTCCAT GAGTGCTAGC 
AGTGGCTTGT CAGCAAGGTC ATCCGCACAG AATGGCAGCG CATTAAAGAG GGAATTCTCT 
GGAGGAAGCT ACAGTGCCAA GCGGCAGCCC ATGCCCTCCC CGTCAGAGGG CTCTTTGTCC 
TCTGGAGGCA TGGACCAAGG AAGTGATGCC CCAGCGAGGG ACTTTGACGG AGAGGACTCT 
GACTCTCCGA GGCATTCCAC AGCTTCCAAC AGTTCCAACC TAAGCAGCCC CCCAAGCCCA 
GCTTCACCCC GAAAAACCAA GAGCCTCTCC CTGGAGAGCA CTGACCGCGG GAGCTGGGAC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858068.1
CDS	1781..7006
Translation	MKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVENTYVWNPSVKFHIQSRSTSPSTSSEAEPVKTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_017002579.1
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002579.1

ORF Insert Sequence:

ATGAAAATAT TGAATAAATG GGAAATGCTG AAAAGAGCTG AGACAGCATG TTTTCGTGAA 
GAAAGGGATG TATTAGTGAA TGGAGACAAT AAATGGATTA CAACCTTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTGCTTACT 
CTACTCAGCA AATTTGAAGA TAGATTGCCT GAAGATATGG CTAGATTTTA CTTGGCTGAG 
ATGGTGATAG CAATTGACTC AGTTCATCAG CTACATTATG TACACAGAGA CATTAAACCT 
GACAATATAC TGATGGATAT GAATGGACAT ATTCGGTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTTCAGTCC TCAGTGGCTG TAGGAACTCC AGATTATATC 
TCTCCTGAAA TCCTTCAAGC CATGGAAGAT GGAAAAGGGA GATATGGACC TGAATGTGAC 
TGGTGGTCTT TGGGGGTCTG TATGTATGAA ATGCTTTACG GAGAAACACC ATTTTATGCA 
GAATCGCTGG TGGAGACATA CGGAAAAATC ATGAACCACA AAGAGAGGTT TCAGTTTCCA 
GCCCAAGTGA CTGATGTGTC TGAAAATGCT AAGGATCTTA TTCGAAGGCT CATTTGTAGC 
AGAGAACATC GACTTGGTCA AAATGGAATA GAAGACTTTA AGAAACACCC ATTTTTCAGT 
GGAATTGATT GGGATAATAT TCGGAACTGT GAAGCACCTT ATATTCCAGA AGTTAGTAGC 
CCAACAGATA CATCGAATTT TGATGTAGAT GATGATTGTT TAAAAAATTC TGAAACGATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CACCATCTGC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTACTTTC TGATCGGAGC TGTTTAAGAG TTACGGCTGG TCCCACCTCA 
CTGGATCTTG ATGTTAATGT TCAGAGGACT CTAGACAACA ACTTAGCAAC TGAAGCTTAT 
GAAAGAAGAA TTAAGCGCCT TGAGCAAGAA AAACTTGAAC TCAGTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTCCAAGC TCTGCAGTAT TCAACTGTTG ATGGTCCACT AACAGCAAGC 
AAAGATTTAG AAATAAAAAA CTTAAAAGAA GAAATTGAAA AACTAAGAAA ACAAGTAACA 
GAATCAAGTC ATTTGGAACA GCAACTTGAA GAAGCTAATG CTGTGAGGCA AGAACTAGAT 
GATGCTTTTA GACAAATCAA GGCTTATGAA AAACAAATCA AAACGTTACA ACAAGAAAGA 
GAAGATCTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGACG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGGCTAACAG AATTGCACAC CCAAAAACAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGTT GAAAGCTTAA GGCAAGAACT GCGCAGAACA 
GAAAGAGCCA AAAAAGAGCT GGAAGTTCAT ACAGAAGCTC TAGCTGCTGA AGCATCTAAA 
GACAGGAAGC TACGTGAACA GAGTGAGCAC TATTCTAAGC AACTGGAAAA TGAATTGGAG 
GGACTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAGTAT GCAGCATAGA ACATCAGCAA 
GAGATAACCA AACTAAAGAC TGATTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAATTA 
TCTAAAAGAG AAGGAATACA TGCAAATGAA ATAAAAAATC TTAAGAAAGA ACTGCATGAT 
TCAGAAGGTC AGCAACTTGC TCTCAACAAA GAAATTATGA TTTTAAAAGA CAAATTGGAA 
AAAACCAGAA GAGAAAGTCA AAGTGAAAGG GAGGAATTTG AAAGTGAGTT CAAACAACAA 
TATGAACGAG AAAAAGTGTT GTTAACTGAA GAAAATAAAA AGCTGACGAG TGAACTTGAT 
AAGCTTACTA CTTTGTATGA GAACTTAAGT ATACACAACC AGCAGTTAGA AGAAGAGGTT 
AAAGATCTAG CAGACAAGAA AGAATCAGTT GCACATTGGG AAGCCCAAAT CACAGAAATA 
ATTCAGTGGG TCAGCGATGA AAAGGATGCA CGAGGGTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AATTGGAGGC ATTAAGAAAT TCCAGCTTGG GTACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCGAAACTG GATATGTCAG CTAGACTGGA GTTGCAGTCG 
GCTCTGGATG CAGAAATAAG AGCCAAACAG GCCATCCAAG AAGAGTTGAA TAAAGTTAAA 
GCATCTAATA TCATAACAGA ATGTAAACTA AAAGATTCAG AGAAGAAGAA CTTGGAACTA 
CTCTCAGAAA TCGAACAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GATCAATTTG AAGATTCCTT TTCTTCTTCC TCATCTTCAC TGATTGATTT TTTGGATGAC 
CGCTCTCCAT CCTGTACTCC AGCTAGCAAA GGCAGACGTA CTGATCCCGT TGAGAACACA 
TATGTATGGA ACCCGAGCGT CAAGTTTCAC ATCCAGTCAC GGTCCACATC TCCTTCCACA 
TCTAGTGAAG CTGAGCCAGT TAAGACTGTA GACTCCACTC CACTTTCAGT TCACACACCA 
ACCTTAAGGA AAAAAGGATG TCCTGGTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAC 
CAGTTTTTTG TAAAATCTTT TACTACTCCT ACCAAGTGTC ATCAGTGTAC CTCCTTGATG 
GTGGGTTTAA TAAGACAGGG CTGTTCATGT GAAGTGTGTG GATTCTCATG CCATATAACT 
TGTGTAAACA AAGCTCCAAC CACTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCCCTG 
GGTATAGATC CTCAGAAAGG AATAGGAACA GCATATGAAG GTCATGTCAG GATTCCTAAG 
CCAGCTGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTA TAGTGTGTGA CTTCAAACTC 
TTTCTGTACG ATATTGCTGA AGGAAAAGCA TCTCAGCCCA GTGTTGTCAT TAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATCCAT 
GCAAGTCGGA AAGATATACC CTGTATATTT AGGGTCACAG CTTCCCAGCT CTCAGCATCT 
AATAACAAAT GTTCAATCCT GATGCTAGCA GACACTGAGA ATGAGAAGAA TAAGTGGGTG 
GGAGTGCTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTACCCCTCA TTAAAACAAC CCAGGCAGCC 
GCAATCATAG ATCATGAAAG AATTGCTTTG GGAAACGAAG AAGGGTTATT TGTTGTACAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATGTCAG CATTGGATGG GCGAGAGACC GATTTTTACA AGCTGTCAGA AACTAAAGGG 
TGTCAAACCG TAACTTCTGG AAAGGTGCGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGAAAATTT 
AAAGAAATTC AAGTCCCATA TAATGTCCAG TGGATGGCAA TCTTCAGTGA ACAACTCTGT 
GTGGGATTCC AGTCAGGATT TCTAAGATAC CCCTTGAATG GAGAAGGAAA TCCATACAGT 
ATGCTCCATT CAAATGACCA TACACTATCA TTTATTGCAC ATCAACCAAT GGATGCTATC 
TGCGCAGTTG AGATCTCCAG TAAAGAATAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAATTGA TGTGGCCAGC AAATCCTTCC 
TCTTGTTGTT ACAATGCACC ATATCTCTCG GTGTACAGTG AAAATGCAGT TGATATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTTCCTCTCA AAAAGGTTCG ACCCTTAAAC 
AATGAAGGAT CATTAAATCT TTTAGGGTTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGACGA ACTGGTAGTA CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ACATTAACAA TAAGCGGCGT TATTCCTTCA GAGTCCCAGA AGAGGAAAGG 
ATGCAGCAGA GGAGGGAAAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAT 
CCAACTAATT TTAATCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTGAAA 
GATCTGCCCA TGCCAGGTTT CCCTTATCCA TCCCCTCATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAACA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGAATATTCC CCGTCTCTGC GCTCTGTCCC TGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGTC GGACAGTATT CAGTGGCTCA 
GTCAGTATTC CATCTATCAC CAAATCCCGC CCTGAGCCAG GCCGCTCCAT GAGTGCTAGC 
AGTGGCTTGT CAGCAAGGTC ATCCGCACAG AATGGCAGCG CATTAAAGAG GGAATTCTCT 
GGAGGAAGCT ACAGTGCCAA GCGGCAGCCC ATGCCCTCCC CGTCAGAGGG CTCTTTGTCC 
TCTGGAGGCA TGGACCAAGG AAGTGATGCC CCAGCGAGGG ACTTTGACGG AGAGGACTCT 
GACTCTCCGA GGCATTCCAC AGCTTCCAAC AGTTCCAACC TAAGCAGCCC CCCAAGCCCA 
GCTTCACCCC GAAAAACCAA GAGCCTCTCC CTGGAGAGCA CTGACCGCGG GAGCTGGGAC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_017002580.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858069.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X14, mRNA.

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CloneID	OHu90669
Clone ID Related Accession (Same CDS sequence)	XM_017002579.1 , XM_017002580.2 , XM_017002580.1 , XM_017002579.2
Accession Version	XM_017002580.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017002580.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATAT TGAATAAATG GGAAATGCTG AAAAGAGCTG AGACAGCATG TTTTCGTGAA 
GAAAGGGATG TATTAGTGAA TGGAGACAAT AAATGGATTA CAACCTTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTGCTTACT 
CTACTCAGCA AATTTGAAGA TAGATTGCCT GAAGATATGG CTAGATTTTA CTTGGCTGAG 
ATGGTGATAG CAATTGACTC AGTTCATCAG CTACATTATG TACACAGAGA CATTAAACCT 
GACAATATAC TGATGGATAT GAATGGACAT ATTCGGTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTTCAGTCC TCAGTGGCTG TAGGAACTCC AGATTATATC 
TCTCCTGAAA TCCTTCAAGC CATGGAAGAT GGAAAAGGGA GATATGGACC TGAATGTGAC 
TGGTGGTCTT TGGGGGTCTG TATGTATGAA ATGCTTTACG GAGAAACACC ATTTTATGCA 
GAATCGCTGG TGGAGACATA CGGAAAAATC ATGAACCACA AAGAGAGGTT TCAGTTTCCA 
GCCCAAGTGA CTGATGTGTC TGAAAATGCT AAGGATCTTA TTCGAAGGCT CATTTGTAGC 
AGAGAACATC GACTTGGTCA AAATGGAATA GAAGACTTTA AGAAACACCC ATTTTTCAGT 
GGAATTGATT GGGATAATAT TCGGAACTGT GAAGCACCTT ATATTCCAGA AGTTAGTAGC 
CCAACAGATA CATCGAATTT TGATGTAGAT GATGATTGTT TAAAAAATTC TGAAACGATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CACCATCTGC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTACTTTC TGATCGGAGC TGTTTAAGAG TTACGGCTGG TCCCACCTCA 
CTGGATCTTG ATGTTAATGT TCAGAGGACT CTAGACAACA ACTTAGCAAC TGAAGCTTAT 
GAAAGAAGAA TTAAGCGCCT TGAGCAAGAA AAACTTGAAC TCAGTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTCCAAGC TCTGCAGTAT TCAACTGTTG ATGGTCCACT AACAGCAAGC 
AAAGATTTAG AAATAAAAAA CTTAAAAGAA GAAATTGAAA AACTAAGAAA ACAAGTAACA 
GAATCAAGTC ATTTGGAACA GCAACTTGAA GAAGCTAATG CTGTGAGGCA AGAACTAGAT 
GATGCTTTTA GACAAATCAA GGCTTATGAA AAACAAATCA AAACGTTACA ACAAGAAAGA 
GAAGATCTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGACG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGGCTAACAG AATTGCACAC CCAAAAACAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGTT GAAAGCTTAA GGCAAGAACT GCGCAGAACA 
GAAAGAGCCA AAAAAGAGCT GGAAGTTCAT ACAGAAGCTC TAGCTGCTGA AGCATCTAAA 
GACAGGAAGC TACGTGAACA GAGTGAGCAC TATTCTAAGC AACTGGAAAA TGAATTGGAG 
GGACTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAGTAT GCAGCATAGA ACATCAGCAA 
GAGATAACCA AACTAAAGAC TGATTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAATTA 
TCTAAAAGAG AAGGAATACA TGCAAATGAA ATAAAAAATC TTAAGAAAGA ACTGCATGAT 
TCAGAAGGTC AGCAACTTGC TCTCAACAAA GAAATTATGA TTTTAAAAGA CAAATTGGAA 
AAAACCAGAA GAGAAAGTCA AAGTGAAAGG GAGGAATTTG AAAGTGAGTT CAAACAACAA 
TATGAACGAG AAAAAGTGTT GTTAACTGAA GAAAATAAAA AGCTGACGAG TGAACTTGAT 
AAGCTTACTA CTTTGTATGA GAACTTAAGT ATACACAACC AGCAGTTAGA AGAAGAGGTT 
AAAGATCTAG CAGACAAGAA AGAATCAGTT GCACATTGGG AAGCCCAAAT CACAGAAATA 
ATTCAGTGGG TCAGCGATGA AAAGGATGCA CGAGGGTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AATTGGAGGC ATTAAGAAAT TCCAGCTTGG GTACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCGAAACTG GATATGTCAG CTAGACTGGA GTTGCAGTCG 
GCTCTGGATG CAGAAATAAG AGCCAAACAG GCCATCCAAG AAGAGTTGAA TAAAGTTAAA 
GCATCTAATA TCATAACAGA ATGTAAACTA AAAGATTCAG AGAAGAAGAA CTTGGAACTA 
CTCTCAGAAA TCGAACAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GATCAATTTG AAGATTCCTT TTCTTCTTCC TCATCTTCAC TGATTGATTT TTTGGATGAC 
CGCTCTCCAT CCTGTACTCC AGCTAGCAAA GGCAGACGTA CTGATCCCGT TGAGAACACA 
TATGTATGGA ACCCGAGCGT CAAGTTTCAC ATCCAGTCAC GGTCCACATC TCCTTCCACA 
TCTAGTGAAG CTGAGCCAGT TAAGACTGTA GACTCCACTC CACTTTCAGT TCACACACCA 
ACCTTAAGGA AAAAAGGATG TCCTGGTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAC 
CAGTTTTTTG TAAAATCTTT TACTACTCCT ACCAAGTGTC ATCAGTGTAC CTCCTTGATG 
GTGGGTTTAA TAAGACAGGG CTGTTCATGT GAAGTGTGTG GATTCTCATG CCATATAACT 
TGTGTAAACA AAGCTCCAAC CACTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCCCTG 
GGTATAGATC CTCAGAAAGG AATAGGAACA GCATATGAAG GTCATGTCAG GATTCCTAAG 
CCAGCTGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTA TAGTGTGTGA CTTCAAACTC 
TTTCTGTACG ATATTGCTGA AGGAAAAGCA TCTCAGCCCA GTGTTGTCAT TAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATCCAT 
GCAAGTCGGA AAGATATACC CTGTATATTT AGGGTCACAG CTTCCCAGCT CTCAGCATCT 
AATAACAAAT GTTCAATCCT GATGCTAGCA GACACTGAGA ATGAGAAGAA TAAGTGGGTG 
GGAGTGCTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTACCCCTCA TTAAAACAAC CCAGGCAGCC 
GCAATCATAG ATCATGAAAG AATTGCTTTG GGAAACGAAG AAGGGTTATT TGTTGTACAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATGTCAG CATTGGATGG GCGAGAGACC GATTTTTACA AGCTGTCAGA AACTAAAGGG 
TGTCAAACCG TAACTTCTGG AAAGGTGCGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGAAAATTT 
AAAGAAATTC AAGTCCCATA TAATGTCCAG TGGATGGCAA TCTTCAGTGA ACAACTCTGT 
GTGGGATTCC AGTCAGGATT TCTAAGATAC CCCTTGAATG GAGAAGGAAA TCCATACAGT 
ATGCTCCATT CAAATGACCA TACACTATCA TTTATTGCAC ATCAACCAAT GGATGCTATC 
TGCGCAGTTG AGATCTCCAG TAAAGAATAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAATTGA TGTGGCCAGC AAATCCTTCC 
TCTTGTTGTT ACAATGCACC ATATCTCTCG GTGTACAGTG AAAATGCAGT TGATATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTTCCTCTCA AAAAGGTTCG ACCCTTAAAC 
AATGAAGGAT CATTAAATCT TTTAGGGTTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGACGA ACTGGTAGTA CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ACATTAACAA TAAGCGGCGT TATTCCTTCA GAGTCCCAGA AGAGGAAAGG 
ATGCAGCAGA GGAGGGAAAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAT 
CCAACTAATT TTAATCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTGAAA 
GATCTGCCCA TGCCAGGTTT CCCTTATCCA TCCCCTCATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAACA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGAATATTCC CCGTCTCTGC GCTCTGTCCC TGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGTC GGACAGTATT CAGTGGCTCA 
GTCAGTATTC CATCTATCAC CAAATCCCGC CCTGAGCCAG GCCGCTCCAT GAGTGCTAGC 
AGTGGCTTGT CAGCAAGGTC ATCCGCACAG AATGGCAGCG CATTAAAGAG GGAATTCTCT 
GGAGGAAGCT ACAGTGCCAA GCGGCAGCCC ATGCCCTCCC CGTCAGAGGG CTCTTTGTCC 
TCTGGAGGCA TGGACCAAGG AAGTGATGCC CCAGCGAGGG ACTTTGACGG AGAGGACTCT 
GACTCTCCGA GGCATTCCAC AGCTTCCAAC AGTTCCAACC TAAGCAGCCC CCCAAGCCCA 
GCTTCACCCC GAAAAACCAA GAGCCTCTCC CTGGAGAGCA CTGACCGCGG GAGCTGGGAC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858069.1
CDS	1541..6766
Translation	MKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVENTYVWNPSVKFHIQSRSTSPSTSSEAEPVKTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_017002580.2
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002580.2

ORF Insert Sequence:

ATGAAAATAT TGAATAAATG GGAAATGCTG AAAAGAGCTG AGACAGCATG TTTTCGTGAA 
GAAAGGGATG TATTAGTGAA TGGAGACAAT AAATGGATTA CAACCTTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTGCTTACT 
CTACTCAGCA AATTTGAAGA TAGATTGCCT GAAGATATGG CTAGATTTTA CTTGGCTGAG 
ATGGTGATAG CAATTGACTC AGTTCATCAG CTACATTATG TACACAGAGA CATTAAACCT 
GACAATATAC TGATGGATAT GAATGGACAT ATTCGGTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTTCAGTCC TCAGTGGCTG TAGGAACTCC AGATTATATC 
TCTCCTGAAA TCCTTCAAGC CATGGAAGAT GGAAAAGGGA GATATGGACC TGAATGTGAC 
TGGTGGTCTT TGGGGGTCTG TATGTATGAA ATGCTTTACG GAGAAACACC ATTTTATGCA 
GAATCGCTGG TGGAGACATA CGGAAAAATC ATGAACCACA AAGAGAGGTT TCAGTTTCCA 
GCCCAAGTGA CTGATGTGTC TGAAAATGCT AAGGATCTTA TTCGAAGGCT CATTTGTAGC 
AGAGAACATC GACTTGGTCA AAATGGAATA GAAGACTTTA AGAAACACCC ATTTTTCAGT 
GGAATTGATT GGGATAATAT TCGGAACTGT GAAGCACCTT ATATTCCAGA AGTTAGTAGC 
CCAACAGATA CATCGAATTT TGATGTAGAT GATGATTGTT TAAAAAATTC TGAAACGATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CACCATCTGC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTACTTTC TGATCGGAGC TGTTTAAGAG TTACGGCTGG TCCCACCTCA 
CTGGATCTTG ATGTTAATGT TCAGAGGACT CTAGACAACA ACTTAGCAAC TGAAGCTTAT 
GAAAGAAGAA TTAAGCGCCT TGAGCAAGAA AAACTTGAAC TCAGTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTCCAAGC TCTGCAGTAT TCAACTGTTG ATGGTCCACT AACAGCAAGC 
AAAGATTTAG AAATAAAAAA CTTAAAAGAA GAAATTGAAA AACTAAGAAA ACAAGTAACA 
GAATCAAGTC ATTTGGAACA GCAACTTGAA GAAGCTAATG CTGTGAGGCA AGAACTAGAT 
GATGCTTTTA GACAAATCAA GGCTTATGAA AAACAAATCA AAACGTTACA ACAAGAAAGA 
GAAGATCTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGACG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGGCTAACAG AATTGCACAC CCAAAAACAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGTT GAAAGCTTAA GGCAAGAACT GCGCAGAACA 
GAAAGAGCCA AAAAAGAGCT GGAAGTTCAT ACAGAAGCTC TAGCTGCTGA AGCATCTAAA 
GACAGGAAGC TACGTGAACA GAGTGAGCAC TATTCTAAGC AACTGGAAAA TGAATTGGAG 
GGACTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAGTAT GCAGCATAGA ACATCAGCAA 
GAGATAACCA AACTAAAGAC TGATTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAATTA 
TCTAAAAGAG AAGGAATACA TGCAAATGAA ATAAAAAATC TTAAGAAAGA ACTGCATGAT 
TCAGAAGGTC AGCAACTTGC TCTCAACAAA GAAATTATGA TTTTAAAAGA CAAATTGGAA 
AAAACCAGAA GAGAAAGTCA AAGTGAAAGG GAGGAATTTG AAAGTGAGTT CAAACAACAA 
TATGAACGAG AAAAAGTGTT GTTAACTGAA GAAAATAAAA AGCTGACGAG TGAACTTGAT 
AAGCTTACTA CTTTGTATGA GAACTTAAGT ATACACAACC AGCAGTTAGA AGAAGAGGTT 
AAAGATCTAG CAGACAAGAA AGAATCAGTT GCACATTGGG AAGCCCAAAT CACAGAAATA 
ATTCAGTGGG TCAGCGATGA AAAGGATGCA CGAGGGTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AATTGGAGGC ATTAAGAAAT TCCAGCTTGG GTACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCGAAACTG GATATGTCAG CTAGACTGGA GTTGCAGTCG 
GCTCTGGATG CAGAAATAAG AGCCAAACAG GCCATCCAAG AAGAGTTGAA TAAAGTTAAA 
GCATCTAATA TCATAACAGA ATGTAAACTA AAAGATTCAG AGAAGAAGAA CTTGGAACTA 
CTCTCAGAAA TCGAACAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GATCAATTTG AAGATTCCTT TTCTTCTTCC TCATCTTCAC TGATTGATTT TTTGGATGAC 
CGCTCTCCAT CCTGTACTCC AGCTAGCAAA GGCAGACGTA CTGATCCCGT TGAGAACACA 
TATGTATGGA ACCCGAGCGT CAAGTTTCAC ATCCAGTCAC GGTCCACATC TCCTTCCACA 
TCTAGTGAAG CTGAGCCAGT TAAGACTGTA GACTCCACTC CACTTTCAGT TCACACACCA 
ACCTTAAGGA AAAAAGGATG TCCTGGTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAC 
CAGTTTTTTG TAAAATCTTT TACTACTCCT ACCAAGTGTC ATCAGTGTAC CTCCTTGATG 
GTGGGTTTAA TAAGACAGGG CTGTTCATGT GAAGTGTGTG GATTCTCATG CCATATAACT 
TGTGTAAACA AAGCTCCAAC CACTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCCCTG 
GGTATAGATC CTCAGAAAGG AATAGGAACA GCATATGAAG GTCATGTCAG GATTCCTAAG 
CCAGCTGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTA TAGTGTGTGA CTTCAAACTC 
TTTCTGTACG ATATTGCTGA AGGAAAAGCA TCTCAGCCCA GTGTTGTCAT TAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATCCAT 
GCAAGTCGGA AAGATATACC CTGTATATTT AGGGTCACAG CTTCCCAGCT CTCAGCATCT 
AATAACAAAT GTTCAATCCT GATGCTAGCA GACACTGAGA ATGAGAAGAA TAAGTGGGTG 
GGAGTGCTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTACCCCTCA TTAAAACAAC CCAGGCAGCC 
GCAATCATAG ATCATGAAAG AATTGCTTTG GGAAACGAAG AAGGGTTATT TGTTGTACAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATGTCAG CATTGGATGG GCGAGAGACC GATTTTTACA AGCTGTCAGA AACTAAAGGG 
TGTCAAACCG TAACTTCTGG AAAGGTGCGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGAAAATTT 
AAAGAAATTC AAGTCCCATA TAATGTCCAG TGGATGGCAA TCTTCAGTGA ACAACTCTGT 
GTGGGATTCC AGTCAGGATT TCTAAGATAC CCCTTGAATG GAGAAGGAAA TCCATACAGT 
ATGCTCCATT CAAATGACCA TACACTATCA TTTATTGCAC ATCAACCAAT GGATGCTATC 
TGCGCAGTTG AGATCTCCAG TAAAGAATAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAATTGA TGTGGCCAGC AAATCCTTCC 
TCTTGTTGTT ACAATGCACC ATATCTCTCG GTGTACAGTG AAAATGCAGT TGATATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTTCCTCTCA AAAAGGTTCG ACCCTTAAAC 
AATGAAGGAT CATTAAATCT TTTAGGGTTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGACGA ACTGGTAGTA CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ACATTAACAA TAAGCGGCGT TATTCCTTCA GAGTCCCAGA AGAGGAAAGG 
ATGCAGCAGA GGAGGGAAAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAT 
CCAACTAATT TTAATCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTGAAA 
GATCTGCCCA TGCCAGGTTT CCCTTATCCA TCCCCTCATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAACA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGAATATTCC CCGTCTCTGC GCTCTGTCCC TGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGTC GGACAGTATT CAGTGGCTCA 
GTCAGTATTC CATCTATCAC CAAATCCCGC CCTGAGCCAG GCCGCTCCAT GAGTGCTAGC 
AGTGGCTTGT CAGCAAGGTC ATCCGCACAG AATGGCAGCG CATTAAAGAG GGAATTCTCT 
GGAGGAAGCT ACAGTGCCAA GCGGCAGCCC ATGCCCTCCC CGTCAGAGGG CTCTTTGTCC 
TCTGGAGGCA TGGACCAAGG AAGTGATGCC CCAGCGAGGG ACTTTGACGG AGAGGACTCT 
GACTCTCCGA GGCATTCCAC AGCTTCCAAC AGTTCCAACC TAAGCAGCCC CCCAAGCCCA 
GCTTCACCCC GAAAAACCAA GAGCCTCTCC CTGGAGAGCA CTGACCGCGG GAGCTGGGAC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_017002580.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858069.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X14, mRNA.

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CloneID	OHu90669
Clone ID Related Accession (Same CDS sequence)	XM_017002579.1 , XM_017002580.2 , XM_017002580.1 , XM_017002579.2
Accession Version	XM_017002580.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATAT TGAATAAATG GGAAATGCTG AAAAGAGCTG AGACAGCATG TTTTCGTGAA 
GAAAGGGATG TATTAGTGAA TGGAGACAAT AAATGGATTA CAACCTTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTGCTTACT 
CTACTCAGCA AATTTGAAGA TAGATTGCCT GAAGATATGG CTAGATTTTA CTTGGCTGAG 
ATGGTGATAG CAATTGACTC AGTTCATCAG CTACATTATG TACACAGAGA CATTAAACCT 
GACAATATAC TGATGGATAT GAATGGACAT ATTCGGTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTTCAGTCC TCAGTGGCTG TAGGAACTCC AGATTATATC 
TCTCCTGAAA TCCTTCAAGC CATGGAAGAT GGAAAAGGGA GATATGGACC TGAATGTGAC 
TGGTGGTCTT TGGGGGTCTG TATGTATGAA ATGCTTTACG GAGAAACACC ATTTTATGCA 
GAATCGCTGG TGGAGACATA CGGAAAAATC ATGAACCACA AAGAGAGGTT TCAGTTTCCA 
GCCCAAGTGA CTGATGTGTC TGAAAATGCT AAGGATCTTA TTCGAAGGCT CATTTGTAGC 
AGAGAACATC GACTTGGTCA AAATGGAATA GAAGACTTTA AGAAACACCC ATTTTTCAGT 
GGAATTGATT GGGATAATAT TCGGAACTGT GAAGCACCTT ATATTCCAGA AGTTAGTAGC 
CCAACAGATA CATCGAATTT TGATGTAGAT GATGATTGTT TAAAAAATTC TGAAACGATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CACCATCTGC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTACTTTC TGATCGGAGC TGTTTAAGAG TTACGGCTGG TCCCACCTCA 
CTGGATCTTG ATGTTAATGT TCAGAGGACT CTAGACAACA ACTTAGCAAC TGAAGCTTAT 
GAAAGAAGAA TTAAGCGCCT TGAGCAAGAA AAACTTGAAC TCAGTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTCCAAGC TCTGCAGTAT TCAACTGTTG ATGGTCCACT AACAGCAAGC 
AAAGATTTAG AAATAAAAAA CTTAAAAGAA GAAATTGAAA AACTAAGAAA ACAAGTAACA 
GAATCAAGTC ATTTGGAACA GCAACTTGAA GAAGCTAATG CTGTGAGGCA AGAACTAGAT 
GATGCTTTTA GACAAATCAA GGCTTATGAA AAACAAATCA AAACGTTACA ACAAGAAAGA 
GAAGATCTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGACG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGGCTAACAG AATTGCACAC CCAAAAACAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGTT GAAAGCTTAA GGCAAGAACT GCGCAGAACA 
GAAAGAGCCA AAAAAGAGCT GGAAGTTCAT ACAGAAGCTC TAGCTGCTGA AGCATCTAAA 
GACAGGAAGC TACGTGAACA GAGTGAGCAC TATTCTAAGC AACTGGAAAA TGAATTGGAG 
GGACTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAGTAT GCAGCATAGA ACATCAGCAA 
GAGATAACCA AACTAAAGAC TGATTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAATTA 
TCTAAAAGAG AAGGAATACA TGCAAATGAA ATAAAAAATC TTAAGAAAGA ACTGCATGAT 
TCAGAAGGTC AGCAACTTGC TCTCAACAAA GAAATTATGA TTTTAAAAGA CAAATTGGAA 
AAAACCAGAA GAGAAAGTCA AAGTGAAAGG GAGGAATTTG AAAGTGAGTT CAAACAACAA 
TATGAACGAG AAAAAGTGTT GTTAACTGAA GAAAATAAAA AGCTGACGAG TGAACTTGAT 
AAGCTTACTA CTTTGTATGA GAACTTAAGT ATACACAACC AGCAGTTAGA AGAAGAGGTT 
AAAGATCTAG CAGACAAGAA AGAATCAGTT GCACATTGGG AAGCCCAAAT CACAGAAATA 
ATTCAGTGGG TCAGCGATGA AAAGGATGCA CGAGGGTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AATTGGAGGC ATTAAGAAAT TCCAGCTTGG GTACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCGAAACTG GATATGTCAG CTAGACTGGA GTTGCAGTCG 
GCTCTGGATG CAGAAATAAG AGCCAAACAG GCCATCCAAG AAGAGTTGAA TAAAGTTAAA 
GCATCTAATA TCATAACAGA ATGTAAACTA AAAGATTCAG AGAAGAAGAA CTTGGAACTA 
CTCTCAGAAA TCGAACAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GATCAATTTG AAGATTCCTT TTCTTCTTCC TCATCTTCAC TGATTGATTT TTTGGATGAC 
CGCTCTCCAT CCTGTACTCC AGCTAGCAAA GGCAGACGTA CTGATCCCGT TGAGAACACA 
TATGTATGGA ACCCGAGCGT CAAGTTTCAC ATCCAGTCAC GGTCCACATC TCCTTCCACA 
TCTAGTGAAG CTGAGCCAGT TAAGACTGTA GACTCCACTC CACTTTCAGT TCACACACCA 
ACCTTAAGGA AAAAAGGATG TCCTGGTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAC 
CAGTTTTTTG TAAAATCTTT TACTACTCCT ACCAAGTGTC ATCAGTGTAC CTCCTTGATG 
GTGGGTTTAA TAAGACAGGG CTGTTCATGT GAAGTGTGTG GATTCTCATG CCATATAACT 
TGTGTAAACA AAGCTCCAAC CACTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCCCTG 
GGTATAGATC CTCAGAAAGG AATAGGAACA GCATATGAAG GTCATGTCAG GATTCCTAAG 
CCAGCTGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTA TAGTGTGTGA CTTCAAACTC 
TTTCTGTACG ATATTGCTGA AGGAAAAGCA TCTCAGCCCA GTGTTGTCAT TAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATCCAT 
GCAAGTCGGA AAGATATACC CTGTATATTT AGGGTCACAG CTTCCCAGCT CTCAGCATCT 
AATAACAAAT GTTCAATCCT GATGCTAGCA GACACTGAGA ATGAGAAGAA TAAGTGGGTG 
GGAGTGCTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTACCCCTCA TTAAAACAAC CCAGGCAGCC 
GCAATCATAG ATCATGAAAG AATTGCTTTG GGAAACGAAG AAGGGTTATT TGTTGTACAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATGTCAG CATTGGATGG GCGAGAGACC GATTTTTACA AGCTGTCAGA AACTAAAGGG 
TGTCAAACCG TAACTTCTGG AAAGGTGCGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGAAAATTT 
AAAGAAATTC AAGTCCCATA TAATGTCCAG TGGATGGCAA TCTTCAGTGA ACAACTCTGT 
GTGGGATTCC AGTCAGGATT TCTAAGATAC CCCTTGAATG GAGAAGGAAA TCCATACAGT 
ATGCTCCATT CAAATGACCA TACACTATCA TTTATTGCAC ATCAACCAAT GGATGCTATC 
TGCGCAGTTG AGATCTCCAG TAAAGAATAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAATTGA TGTGGCCAGC AAATCCTTCC 
TCTTGTTGTT ACAATGCACC ATATCTCTCG GTGTACAGTG AAAATGCAGT TGATATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTTCCTCTCA AAAAGGTTCG ACCCTTAAAC 
AATGAAGGAT CATTAAATCT TTTAGGGTTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGACGA ACTGGTAGTA CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ACATTAACAA TAAGCGGCGT TATTCCTTCA GAGTCCCAGA AGAGGAAAGG 
ATGCAGCAGA GGAGGGAAAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAT 
CCAACTAATT TTAATCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTGAAA 
GATCTGCCCA TGCCAGGTTT CCCTTATCCA TCCCCTCATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAACA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGAATATTCC CCGTCTCTGC GCTCTGTCCC TGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGTC GGACAGTATT CAGTGGCTCA 
GTCAGTATTC CATCTATCAC CAAATCCCGC CCTGAGCCAG GCCGCTCCAT GAGTGCTAGC 
AGTGGCTTGT CAGCAAGGTC ATCCGCACAG AATGGCAGCG CATTAAAGAG GGAATTCTCT 
GGAGGAAGCT ACAGTGCCAA GCGGCAGCCC ATGCCCTCCC CGTCAGAGGG CTCTTTGTCC 
TCTGGAGGCA TGGACCAAGG AAGTGATGCC CCAGCGAGGG ACTTTGACGG AGAGGACTCT 
GACTCTCCGA GGCATTCCAC AGCTTCCAAC AGTTCCAACC TAAGCAGCCC CCCAAGCCCA 
GCTTCACCCC GAAAAACCAA GAGCCTCTCC CTGGAGAGCA CTGACCGCGG GAGCTGGGAC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858069.1
CDS	68..5293
Translation	MKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVENTYVWNPSVKFHIQSRSTSPSTSSEAEPVKTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_017002580.1
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002580.1

ORF Insert Sequence:

ATGAAAATAT TGAATAAATG GGAAATGCTG AAAAGAGCTG AGACAGCATG TTTTCGTGAA 
GAAAGGGATG TATTAGTGAA TGGAGACAAT AAATGGATTA CAACCTTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTGCTTACT 
CTACTCAGCA AATTTGAAGA TAGATTGCCT GAAGATATGG CTAGATTTTA CTTGGCTGAG 
ATGGTGATAG CAATTGACTC AGTTCATCAG CTACATTATG TACACAGAGA CATTAAACCT 
GACAATATAC TGATGGATAT GAATGGACAT ATTCGGTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTTCAGTCC TCAGTGGCTG TAGGAACTCC AGATTATATC 
TCTCCTGAAA TCCTTCAAGC CATGGAAGAT GGAAAAGGGA GATATGGACC TGAATGTGAC 
TGGTGGTCTT TGGGGGTCTG TATGTATGAA ATGCTTTACG GAGAAACACC ATTTTATGCA 
GAATCGCTGG TGGAGACATA CGGAAAAATC ATGAACCACA AAGAGAGGTT TCAGTTTCCA 
GCCCAAGTGA CTGATGTGTC TGAAAATGCT AAGGATCTTA TTCGAAGGCT CATTTGTAGC 
AGAGAACATC GACTTGGTCA AAATGGAATA GAAGACTTTA AGAAACACCC ATTTTTCAGT 
GGAATTGATT GGGATAATAT TCGGAACTGT GAAGCACCTT ATATTCCAGA AGTTAGTAGC 
CCAACAGATA CATCGAATTT TGATGTAGAT GATGATTGTT TAAAAAATTC TGAAACGATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CACCATCTGC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTACTTTC TGATCGGAGC TGTTTAAGAG TTACGGCTGG TCCCACCTCA 
CTGGATCTTG ATGTTAATGT TCAGAGGACT CTAGACAACA ACTTAGCAAC TGAAGCTTAT 
GAAAGAAGAA TTAAGCGCCT TGAGCAAGAA AAACTTGAAC TCAGTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTCCAAGC TCTGCAGTAT TCAACTGTTG ATGGTCCACT AACAGCAAGC 
AAAGATTTAG AAATAAAAAA CTTAAAAGAA GAAATTGAAA AACTAAGAAA ACAAGTAACA 
GAATCAAGTC ATTTGGAACA GCAACTTGAA GAAGCTAATG CTGTGAGGCA AGAACTAGAT 
GATGCTTTTA GACAAATCAA GGCTTATGAA AAACAAATCA AAACGTTACA ACAAGAAAGA 
GAAGATCTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGACG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGGCTAACAG AATTGCACAC CCAAAAACAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGTT GAAAGCTTAA GGCAAGAACT GCGCAGAACA 
GAAAGAGCCA AAAAAGAGCT GGAAGTTCAT ACAGAAGCTC TAGCTGCTGA AGCATCTAAA 
GACAGGAAGC TACGTGAACA GAGTGAGCAC TATTCTAAGC AACTGGAAAA TGAATTGGAG 
GGACTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAGTAT GCAGCATAGA ACATCAGCAA 
GAGATAACCA AACTAAAGAC TGATTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAATTA 
TCTAAAAGAG AAGGAATACA TGCAAATGAA ATAAAAAATC TTAAGAAAGA ACTGCATGAT 
TCAGAAGGTC AGCAACTTGC TCTCAACAAA GAAATTATGA TTTTAAAAGA CAAATTGGAA 
AAAACCAGAA GAGAAAGTCA AAGTGAAAGG GAGGAATTTG AAAGTGAGTT CAAACAACAA 
TATGAACGAG AAAAAGTGTT GTTAACTGAA GAAAATAAAA AGCTGACGAG TGAACTTGAT 
AAGCTTACTA CTTTGTATGA GAACTTAAGT ATACACAACC AGCAGTTAGA AGAAGAGGTT 
AAAGATCTAG CAGACAAGAA AGAATCAGTT GCACATTGGG AAGCCCAAAT CACAGAAATA 
ATTCAGTGGG TCAGCGATGA AAAGGATGCA CGAGGGTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AATTGGAGGC ATTAAGAAAT TCCAGCTTGG GTACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCGAAACTG GATATGTCAG CTAGACTGGA GTTGCAGTCG 
GCTCTGGATG CAGAAATAAG AGCCAAACAG GCCATCCAAG AAGAGTTGAA TAAAGTTAAA 
GCATCTAATA TCATAACAGA ATGTAAACTA AAAGATTCAG AGAAGAAGAA CTTGGAACTA 
CTCTCAGAAA TCGAACAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GATCAATTTG AAGATTCCTT TTCTTCTTCC TCATCTTCAC TGATTGATTT TTTGGATGAC 
CGCTCTCCAT CCTGTACTCC AGCTAGCAAA GGCAGACGTA CTGATCCCGT TGAGAACACA 
TATGTATGGA ACCCGAGCGT CAAGTTTCAC ATCCAGTCAC GGTCCACATC TCCTTCCACA 
TCTAGTGAAG CTGAGCCAGT TAAGACTGTA GACTCCACTC CACTTTCAGT TCACACACCA 
ACCTTAAGGA AAAAAGGATG TCCTGGTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAC 
CAGTTTTTTG TAAAATCTTT TACTACTCCT ACCAAGTGTC ATCAGTGTAC CTCCTTGATG 
GTGGGTTTAA TAAGACAGGG CTGTTCATGT GAAGTGTGTG GATTCTCATG CCATATAACT 
TGTGTAAACA AAGCTCCAAC CACTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCCCTG 
GGTATAGATC CTCAGAAAGG AATAGGAACA GCATATGAAG GTCATGTCAG GATTCCTAAG 
CCAGCTGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTA TAGTGTGTGA CTTCAAACTC 
TTTCTGTACG ATATTGCTGA AGGAAAAGCA TCTCAGCCCA GTGTTGTCAT TAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATCCAT 
GCAAGTCGGA AAGATATACC CTGTATATTT AGGGTCACAG CTTCCCAGCT CTCAGCATCT 
AATAACAAAT GTTCAATCCT GATGCTAGCA GACACTGAGA ATGAGAAGAA TAAGTGGGTG 
GGAGTGCTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTACCCCTCA TTAAAACAAC CCAGGCAGCC 
GCAATCATAG ATCATGAAAG AATTGCTTTG GGAAACGAAG AAGGGTTATT TGTTGTACAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATGTCAG CATTGGATGG GCGAGAGACC GATTTTTACA AGCTGTCAGA AACTAAAGGG 
TGTCAAACCG TAACTTCTGG AAAGGTGCGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGAAAATTT 
AAAGAAATTC AAGTCCCATA TAATGTCCAG TGGATGGCAA TCTTCAGTGA ACAACTCTGT 
GTGGGATTCC AGTCAGGATT TCTAAGATAC CCCTTGAATG GAGAAGGAAA TCCATACAGT 
ATGCTCCATT CAAATGACCA TACACTATCA TTTATTGCAC ATCAACCAAT GGATGCTATC 
TGCGCAGTTG AGATCTCCAG TAAAGAATAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAATTGA TGTGGCCAGC AAATCCTTCC 
TCTTGTTGTT ACAATGCACC ATATCTCTCG GTGTACAGTG AAAATGCAGT TGATATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTTCCTCTCA AAAAGGTTCG ACCCTTAAAC 
AATGAAGGAT CATTAAATCT TTTAGGGTTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGACGA ACTGGTAGTA CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ACATTAACAA TAAGCGGCGT TATTCCTTCA GAGTCCCAGA AGAGGAAAGG 
ATGCAGCAGA GGAGGGAAAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAT 
CCAACTAATT TTAATCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTGAAA 
GATCTGCCCA TGCCAGGTTT CCCTTATCCA TCCCCTCATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAACA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGAATATTCC CCGTCTCTGC GCTCTGTCCC TGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGTC GGACAGTATT CAGTGGCTCA 
GTCAGTATTC CATCTATCAC CAAATCCCGC CCTGAGCCAG GCCGCTCCAT GAGTGCTAGC 
AGTGGCTTGT CAGCAAGGTC ATCCGCACAG AATGGCAGCG CATTAAAGAG GGAATTCTCT 
GGAGGAAGCT ACAGTGCCAA GCGGCAGCCC ATGCCCTCCC CGTCAGAGGG CTCTTTGTCC 
TCTGGAGGCA TGGACCAAGG AAGTGATGCC CCAGCGAGGG ACTTTGACGG AGAGGACTCT 
GACTCTCCGA GGCATTCCAC AGCTTCCAAC AGTTCCAACC TAAGCAGCCC CCCAAGCCCA 
GCTTCACCCC GAAAAACCAA GAGCCTCTCC CTGGAGAGCA CTGACCGCGG GAGCTGGGAC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_017002579.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858068.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X13, mRNA.

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CloneID	OHu90669
Clone ID Related Accession (Same CDS sequence)	XM_017002579.1 , XM_017002580.2 , XM_017002580.1 , XM_017002579.2
Accession Version	XM_017002579.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017002579.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATAT TGAATAAATG GGAAATGCTG AAAAGAGCTG AGACAGCATG TTTTCGTGAA 
GAAAGGGATG TATTAGTGAA TGGAGACAAT AAATGGATTA CAACCTTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTGCTTACT 
CTACTCAGCA AATTTGAAGA TAGATTGCCT GAAGATATGG CTAGATTTTA CTTGGCTGAG 
ATGGTGATAG CAATTGACTC AGTTCATCAG CTACATTATG TACACAGAGA CATTAAACCT 
GACAATATAC TGATGGATAT GAATGGACAT ATTCGGTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTTCAGTCC TCAGTGGCTG TAGGAACTCC AGATTATATC 
TCTCCTGAAA TCCTTCAAGC CATGGAAGAT GGAAAAGGGA GATATGGACC TGAATGTGAC 
TGGTGGTCTT TGGGGGTCTG TATGTATGAA ATGCTTTACG GAGAAACACC ATTTTATGCA 
GAATCGCTGG TGGAGACATA CGGAAAAATC ATGAACCACA AAGAGAGGTT TCAGTTTCCA 
GCCCAAGTGA CTGATGTGTC TGAAAATGCT AAGGATCTTA TTCGAAGGCT CATTTGTAGC 
AGAGAACATC GACTTGGTCA AAATGGAATA GAAGACTTTA AGAAACACCC ATTTTTCAGT 
GGAATTGATT GGGATAATAT TCGGAACTGT GAAGCACCTT ATATTCCAGA AGTTAGTAGC 
CCAACAGATA CATCGAATTT TGATGTAGAT GATGATTGTT TAAAAAATTC TGAAACGATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CACCATCTGC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTACTTTC TGATCGGAGC TGTTTAAGAG TTACGGCTGG TCCCACCTCA 
CTGGATCTTG ATGTTAATGT TCAGAGGACT CTAGACAACA ACTTAGCAAC TGAAGCTTAT 
GAAAGAAGAA TTAAGCGCCT TGAGCAAGAA AAACTTGAAC TCAGTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTCCAAGC TCTGCAGTAT TCAACTGTTG ATGGTCCACT AACAGCAAGC 
AAAGATTTAG AAATAAAAAA CTTAAAAGAA GAAATTGAAA AACTAAGAAA ACAAGTAACA 
GAATCAAGTC ATTTGGAACA GCAACTTGAA GAAGCTAATG CTGTGAGGCA AGAACTAGAT 
GATGCTTTTA GACAAATCAA GGCTTATGAA AAACAAATCA AAACGTTACA ACAAGAAAGA 
GAAGATCTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGACG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGGCTAACAG AATTGCACAC CCAAAAACAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGTT GAAAGCTTAA GGCAAGAACT GCGCAGAACA 
GAAAGAGCCA AAAAAGAGCT GGAAGTTCAT ACAGAAGCTC TAGCTGCTGA AGCATCTAAA 
GACAGGAAGC TACGTGAACA GAGTGAGCAC TATTCTAAGC AACTGGAAAA TGAATTGGAG 
GGACTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAGTAT GCAGCATAGA ACATCAGCAA 
GAGATAACCA AACTAAAGAC TGATTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAATTA 
TCTAAAAGAG AAGGAATACA TGCAAATGAA ATAAAAAATC TTAAGAAAGA ACTGCATGAT 
TCAGAAGGTC AGCAACTTGC TCTCAACAAA GAAATTATGA TTTTAAAAGA CAAATTGGAA 
AAAACCAGAA GAGAAAGTCA AAGTGAAAGG GAGGAATTTG AAAGTGAGTT CAAACAACAA 
TATGAACGAG AAAAAGTGTT GTTAACTGAA GAAAATAAAA AGCTGACGAG TGAACTTGAT 
AAGCTTACTA CTTTGTATGA GAACTTAAGT ATACACAACC AGCAGTTAGA AGAAGAGGTT 
AAAGATCTAG CAGACAAGAA AGAATCAGTT GCACATTGGG AAGCCCAAAT CACAGAAATA 
ATTCAGTGGG TCAGCGATGA AAAGGATGCA CGAGGGTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AATTGGAGGC ATTAAGAAAT TCCAGCTTGG GTACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCGAAACTG GATATGTCAG CTAGACTGGA GTTGCAGTCG 
GCTCTGGATG CAGAAATAAG AGCCAAACAG GCCATCCAAG AAGAGTTGAA TAAAGTTAAA 
GCATCTAATA TCATAACAGA ATGTAAACTA AAAGATTCAG AGAAGAAGAA CTTGGAACTA 
CTCTCAGAAA TCGAACAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GATCAATTTG AAGATTCCTT TTCTTCTTCC TCATCTTCAC TGATTGATTT TTTGGATGAC 
CGCTCTCCAT CCTGTACTCC AGCTAGCAAA GGCAGACGTA CTGATCCCGT TGAGAACACA 
TATGTATGGA ACCCGAGCGT CAAGTTTCAC ATCCAGTCAC GGTCCACATC TCCTTCCACA 
TCTAGTGAAG CTGAGCCAGT TAAGACTGTA GACTCCACTC CACTTTCAGT TCACACACCA 
ACCTTAAGGA AAAAAGGATG TCCTGGTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAC 
CAGTTTTTTG TAAAATCTTT TACTACTCCT ACCAAGTGTC ATCAGTGTAC CTCCTTGATG 
GTGGGTTTAA TAAGACAGGG CTGTTCATGT GAAGTGTGTG GATTCTCATG CCATATAACT 
TGTGTAAACA AAGCTCCAAC CACTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCCCTG 
GGTATAGATC CTCAGAAAGG AATAGGAACA GCATATGAAG GTCATGTCAG GATTCCTAAG 
CCAGCTGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTA TAGTGTGTGA CTTCAAACTC 
TTTCTGTACG ATATTGCTGA AGGAAAAGCA TCTCAGCCCA GTGTTGTCAT TAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATCCAT 
GCAAGTCGGA AAGATATACC CTGTATATTT AGGGTCACAG CTTCCCAGCT CTCAGCATCT 
AATAACAAAT GTTCAATCCT GATGCTAGCA GACACTGAGA ATGAGAAGAA TAAGTGGGTG 
GGAGTGCTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTACCCCTCA TTAAAACAAC CCAGGCAGCC 
GCAATCATAG ATCATGAAAG AATTGCTTTG GGAAACGAAG AAGGGTTATT TGTTGTACAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATGTCAG CATTGGATGG GCGAGAGACC GATTTTTACA AGCTGTCAGA AACTAAAGGG 
TGTCAAACCG TAACTTCTGG AAAGGTGCGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGAAAATTT 
AAAGAAATTC AAGTCCCATA TAATGTCCAG TGGATGGCAA TCTTCAGTGA ACAACTCTGT 
GTGGGATTCC AGTCAGGATT TCTAAGATAC CCCTTGAATG GAGAAGGAAA TCCATACAGT 
ATGCTCCATT CAAATGACCA TACACTATCA TTTATTGCAC ATCAACCAAT GGATGCTATC 
TGCGCAGTTG AGATCTCCAG TAAAGAATAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAATTGA TGTGGCCAGC AAATCCTTCC 
TCTTGTTGTT ACAATGCACC ATATCTCTCG GTGTACAGTG AAAATGCAGT TGATATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTTCCTCTCA AAAAGGTTCG ACCCTTAAAC 
AATGAAGGAT CATTAAATCT TTTAGGGTTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGACGA ACTGGTAGTA CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ACATTAACAA TAAGCGGCGT TATTCCTTCA GAGTCCCAGA AGAGGAAAGG 
ATGCAGCAGA GGAGGGAAAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAT 
CCAACTAATT TTAATCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTGAAA 
GATCTGCCCA TGCCAGGTTT CCCTTATCCA TCCCCTCATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAACA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGAATATTCC CCGTCTCTGC GCTCTGTCCC TGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGTC GGACAGTATT CAGTGGCTCA 
GTCAGTATTC CATCTATCAC CAAATCCCGC CCTGAGCCAG GCCGCTCCAT GAGTGCTAGC 
AGTGGCTTGT CAGCAAGGTC ATCCGCACAG AATGGCAGCG CATTAAAGAG GGAATTCTCT 
GGAGGAAGCT ACAGTGCCAA GCGGCAGCCC ATGCCCTCCC CGTCAGAGGG CTCTTTGTCC 
TCTGGAGGCA TGGACCAAGG AAGTGATGCC CCAGCGAGGG ACTTTGACGG AGAGGACTCT 
GACTCTCCGA GGCATTCCAC AGCTTCCAAC AGTTCCAACC TAAGCAGCCC CCCAAGCCCA 
GCTTCACCCC GAAAAACCAA GAGCCTCTCC CTGGAGAGCA CTGACCGCGG GAGCTGGGAC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858068.1
CDS	1791..7016
Translation	MKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVENTYVWNPSVKFHIQSRSTSPSTSSEAEPVKTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_017002579.2
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002579.2

ORF Insert Sequence:

ATGAAAATAT TGAATAAATG GGAAATGCTG AAAAGAGCTG AGACAGCATG TTTTCGTGAA 
GAAAGGGATG TATTAGTGAA TGGAGACAAT AAATGGATTA CAACCTTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTGCTTACT 
CTACTCAGCA AATTTGAAGA TAGATTGCCT GAAGATATGG CTAGATTTTA CTTGGCTGAG 
ATGGTGATAG CAATTGACTC AGTTCATCAG CTACATTATG TACACAGAGA CATTAAACCT 
GACAATATAC TGATGGATAT GAATGGACAT ATTCGGTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTTCAGTCC TCAGTGGCTG TAGGAACTCC AGATTATATC 
TCTCCTGAAA TCCTTCAAGC CATGGAAGAT GGAAAAGGGA GATATGGACC TGAATGTGAC 
TGGTGGTCTT TGGGGGTCTG TATGTATGAA ATGCTTTACG GAGAAACACC ATTTTATGCA 
GAATCGCTGG TGGAGACATA CGGAAAAATC ATGAACCACA AAGAGAGGTT TCAGTTTCCA 
GCCCAAGTGA CTGATGTGTC TGAAAATGCT AAGGATCTTA TTCGAAGGCT CATTTGTAGC 
AGAGAACATC GACTTGGTCA AAATGGAATA GAAGACTTTA AGAAACACCC ATTTTTCAGT 
GGAATTGATT GGGATAATAT TCGGAACTGT GAAGCACCTT ATATTCCAGA AGTTAGTAGC 
CCAACAGATA CATCGAATTT TGATGTAGAT GATGATTGTT TAAAAAATTC TGAAACGATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CACCATCTGC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTACTTTC TGATCGGAGC TGTTTAAGAG TTACGGCTGG TCCCACCTCA 
CTGGATCTTG ATGTTAATGT TCAGAGGACT CTAGACAACA ACTTAGCAAC TGAAGCTTAT 
GAAAGAAGAA TTAAGCGCCT TGAGCAAGAA AAACTTGAAC TCAGTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTCCAAGC TCTGCAGTAT TCAACTGTTG ATGGTCCACT AACAGCAAGC 
AAAGATTTAG AAATAAAAAA CTTAAAAGAA GAAATTGAAA AACTAAGAAA ACAAGTAACA 
GAATCAAGTC ATTTGGAACA GCAACTTGAA GAAGCTAATG CTGTGAGGCA AGAACTAGAT 
GATGCTTTTA GACAAATCAA GGCTTATGAA AAACAAATCA AAACGTTACA ACAAGAAAGA 
GAAGATCTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGACG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGGCTAACAG AATTGCACAC CCAAAAACAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGTT GAAAGCTTAA GGCAAGAACT GCGCAGAACA 
GAAAGAGCCA AAAAAGAGCT GGAAGTTCAT ACAGAAGCTC TAGCTGCTGA AGCATCTAAA 
GACAGGAAGC TACGTGAACA GAGTGAGCAC TATTCTAAGC AACTGGAAAA TGAATTGGAG 
GGACTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAGTAT GCAGCATAGA ACATCAGCAA 
GAGATAACCA AACTAAAGAC TGATTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAATTA 
TCTAAAAGAG AAGGAATACA TGCAAATGAA ATAAAAAATC TTAAGAAAGA ACTGCATGAT 
TCAGAAGGTC AGCAACTTGC TCTCAACAAA GAAATTATGA TTTTAAAAGA CAAATTGGAA 
AAAACCAGAA GAGAAAGTCA AAGTGAAAGG GAGGAATTTG AAAGTGAGTT CAAACAACAA 
TATGAACGAG AAAAAGTGTT GTTAACTGAA GAAAATAAAA AGCTGACGAG TGAACTTGAT 
AAGCTTACTA CTTTGTATGA GAACTTAAGT ATACACAACC AGCAGTTAGA AGAAGAGGTT 
AAAGATCTAG CAGACAAGAA AGAATCAGTT GCACATTGGG AAGCCCAAAT CACAGAAATA 
ATTCAGTGGG TCAGCGATGA AAAGGATGCA CGAGGGTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AATTGGAGGC ATTAAGAAAT TCCAGCTTGG GTACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCGAAACTG GATATGTCAG CTAGACTGGA GTTGCAGTCG 
GCTCTGGATG CAGAAATAAG AGCCAAACAG GCCATCCAAG AAGAGTTGAA TAAAGTTAAA 
GCATCTAATA TCATAACAGA ATGTAAACTA AAAGATTCAG AGAAGAAGAA CTTGGAACTA 
CTCTCAGAAA TCGAACAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GATCAATTTG AAGATTCCTT TTCTTCTTCC TCATCTTCAC TGATTGATTT TTTGGATGAC 
CGCTCTCCAT CCTGTACTCC AGCTAGCAAA GGCAGACGTA CTGATCCCGT TGAGAACACA 
TATGTATGGA ACCCGAGCGT CAAGTTTCAC ATCCAGTCAC GGTCCACATC TCCTTCCACA 
TCTAGTGAAG CTGAGCCAGT TAAGACTGTA GACTCCACTC CACTTTCAGT TCACACACCA 
ACCTTAAGGA AAAAAGGATG TCCTGGTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAC 
CAGTTTTTTG TAAAATCTTT TACTACTCCT ACCAAGTGTC ATCAGTGTAC CTCCTTGATG 
GTGGGTTTAA TAAGACAGGG CTGTTCATGT GAAGTGTGTG GATTCTCATG CCATATAACT 
TGTGTAAACA AAGCTCCAAC CACTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCCCTG 
GGTATAGATC CTCAGAAAGG AATAGGAACA GCATATGAAG GTCATGTCAG GATTCCTAAG 
CCAGCTGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTA TAGTGTGTGA CTTCAAACTC 
TTTCTGTACG ATATTGCTGA AGGAAAAGCA TCTCAGCCCA GTGTTGTCAT TAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATCCAT 
GCAAGTCGGA AAGATATACC CTGTATATTT AGGGTCACAG CTTCCCAGCT CTCAGCATCT 
AATAACAAAT GTTCAATCCT GATGCTAGCA GACACTGAGA ATGAGAAGAA TAAGTGGGTG 
GGAGTGCTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTACCCCTCA TTAAAACAAC CCAGGCAGCC 
GCAATCATAG ATCATGAAAG AATTGCTTTG GGAAACGAAG AAGGGTTATT TGTTGTACAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATGTCAG CATTGGATGG GCGAGAGACC GATTTTTACA AGCTGTCAGA AACTAAAGGG 
TGTCAAACCG TAACTTCTGG AAAGGTGCGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGAAAATTT 
AAAGAAATTC AAGTCCCATA TAATGTCCAG TGGATGGCAA TCTTCAGTGA ACAACTCTGT 
GTGGGATTCC AGTCAGGATT TCTAAGATAC CCCTTGAATG GAGAAGGAAA TCCATACAGT 
ATGCTCCATT CAAATGACCA TACACTATCA TTTATTGCAC ATCAACCAAT GGATGCTATC 
TGCGCAGTTG AGATCTCCAG TAAAGAATAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAATTGA TGTGGCCAGC AAATCCTTCC 
TCTTGTTGTT ACAATGCACC ATATCTCTCG GTGTACAGTG AAAATGCAGT TGATATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTTCCTCTCA AAAAGGTTCG ACCCTTAAAC 
AATGAAGGAT CATTAAATCT TTTAGGGTTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGACGA ACTGGTAGTA CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ACATTAACAA TAAGCGGCGT TATTCCTTCA GAGTCCCAGA AGAGGAAAGG 
ATGCAGCAGA GGAGGGAAAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAT 
CCAACTAATT TTAATCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTGAAA 
GATCTGCCCA TGCCAGGTTT CCCTTATCCA TCCCCTCATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAACA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGAATATTCC CCGTCTCTGC GCTCTGTCCC TGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGTC GGACAGTATT CAGTGGCTCA 
GTCAGTATTC CATCTATCAC CAAATCCCGC CCTGAGCCAG GCCGCTCCAT GAGTGCTAGC 
AGTGGCTTGT CAGCAAGGTC ATCCGCACAG AATGGCAGCG CATTAAAGAG GGAATTCTCT 
GGAGGAAGCT ACAGTGCCAA GCGGCAGCCC ATGCCCTCCC CGTCAGAGGG CTCTTTGTCC 
TCTGGAGGCA TGGACCAAGG AAGTGATGCC CCAGCGAGGG ACTTTGACGG AGAGGACTCT 
GACTCTCCGA GGCATTCCAC AGCTTCCAAC AGTTCCAACC TAAGCAGCCC CCCAAGCCCA 
GCTTCACCCC GAAAAACCAA GAGCCTCTCC CTGGAGAGCA CTGACCGCGG GAGCTGGGAC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_017002581.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858070.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X16, mRNA.

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CloneID	OHu90670
Clone ID Related Accession (Same CDS sequence)	XM_017002581.1 , XM_017002581.2
Accession Version	XM_017002581.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATAT TGAATAAATG GGAAATGCTG AAAAGAGCTG AGACAGCATG TTTTCGTGAA 
GAAAGGGATG TATTAGTGAA TGGAGACAAT AAATGGATTA CAACCTTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTGCTTACT 
CTACTCAGCA AATTTGAAGA TAGATTGCCT GAAGATATGG CTAGATTTTA CTTGGCTGAG 
ATGGTGATAG CAATTGACTC AGTTCATCAG CTACATTATG TACACAGAGA CATTAAACCT 
GACAATATAC TGATGGATAT GAATGGACAT ATTCGGTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTTCAGTCC TCAGTGGCTG TAGGAACTCC AGATTATATC 
TCTCCTGAAA TCCTTCAAGC CATGGAAGAT GGAAAAGGGA GATATGGACC TGAATGTGAC 
TGGTGGTCTT TGGGGGTCTG TATGTATGAA ATGCTTTACG GAGAAACACC ATTTTATGCA 
GAATCGCTGG TGGAGACATA CGGAAAAATC ATGAACCACA AAGAGAGGTT TCAGTTTCCA 
GCCCAAGTGA CTGATGTGTC TGAAAATGCT AAGGATCTTA TTCGAAGGCT CATTTGTAGC 
AGAGAACATC GACTTGGTCA AAATGGAATA GAAGACTTTA AGAAACACCC ATTTTTCAGT 
GGAATTGATT GGGATAATAT TCGGAACTGT GAAGCACCTT ATATTCCAGA AGTTAGTAGC 
CCAACAGATA CATCGAATTT TGATGTAGAT GATGATTGTT TAAAAAATTC TGAAACGATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CACCATCTGC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTACTTTC TGATCGGAGC TGTTTAAGAG TTACGGCTGG TCCCACCTCA 
CTGGATCTTG ATGTTAATGT TCAGAGGACT CTAGACAACA ACTTAGCAAC TGAAGCTTAT 
GAAAGAAGAA TTAAGCGCCT TGAGCAAGAA AAACTTGAAC TCAGTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTCCAAGC TCTGCAGTAT TCAACTGTTG ATGGTCCACT AACAGCAAGC 
AAAGATTTAG AAATAAAAAA CTTAAAAGAA GAAATTGAAA AACTAAGAAA ACAAGTAACA 
GAATCAAGTC ATTTGGAACA GCAACTTGAA GAAGCTAATG CTGTGAGGCA AGAACTAGAT 
GATGCTTTTA GACAAATCAA GGCTTATGAA AAACAAATCA AAACGTTACA ACAAGAAAGA 
GAAGATCTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGACG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGGCTAACAG AATTGCACAC CCAAAAACAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGTT GAAAGCTTAA GGCAAGAACT GCGCAGAACA 
GAAAGAGCCA AAAAAGAGCT GGAAGTTCAT ACAGAAGCTC TAGCTGCTGA AGCATCTAAA 
GACAGGAAGC TACGTGAACA GAGTGAGCAC TATTCTAAGC AACTGGAAAA TGAATTGGAG 
GGACTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAGTAT GCAGCATAGA ACATCAGCAA 
GAGATAACCA AACTAAAGAC TGATTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAATTA 
TCTAAAAGAG AAGGAATACA TGCAAATGAA ATAAAAAATC TTAAGAAAGA ACTGCATGAT 
TCAGAAGGTC AGCAACTTGC TCTCAACAAA GAAATTATGA TTTTAAAAGA CAAATTGGAA 
AAAACCAGAA GAGAAAGTCA AAGTGAAAGG GAGGAATTTG AAAGTGAGTT CAAACAACAA 
TATGAACGAG AAAAAGTGTT GTTAACTGAA GAAAATAAAA AGCTGACGAG TGAACTTGAT 
AAGCTTACTA CTTTGTATGA GAACTTAAGT ATACACAACC AGCAGTTAGA AGAAGAGGTT 
AAAGATCTAG CAGACAAGAA AGAATCAGTT GCACATTGGG AAGCCCAAAT CACAGAAATA 
ATTCAGTGGG TCAGCGATGA AAAGGATGCA CGAGGGTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AATTGGAGGC ATTAAGAAAT TCCAGCTTGG GTACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCGAAACTG GATATGTCAG CTAGACTGGA GTTGCAGTCG 
GCTCTGGATG CAGAAATAAG AGCCAAACAG GCCATCCAAG AAGAGTTGAA TAAAGTTAAA 
GCATCTAATA TCATAACAGA ATGTAAACTA AAAGATTCAG AGAAGAAGAA CTTGGAACTA 
CTCTCAGAAA TCGAACAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GATCAATTTG AAACTGATCC CGTTGAGAAC ACATATGTAT GGAACCCGAG CGTCAAGTTT 
CACATCCAGT CACGGTCCAC ATCTCCTTCC ACATCTAGTG AAGCTGAGCC AGTTAAGACT 
GTAGACTCCA CTCCACTTTC AGTTCACACA CCAACCTTAA GGAAAAAAGG ATGTCCTGGT 
TCAACTGGCT TTCCACCTAA GCGCAAGACT CACCAGTTTT TTGTAAAATC TTTTACTACT 
CCTACCAAGT GTCATCAGTG TACCTCCTTG ATGGTGGGTT TAATAAGACA GGGCTGTTCA 
TGTGAAGTGT GTGGATTCTC ATGCCATATA ACTTGTGTAA ACAAAGCTCC AACCACTTGT 
CCAGTTCCTC CTGAACAGAC AAAAGGTCCC CTGGGTATAG ATCCTCAGAA AGGAATAGGA 
ACAGCATATG AAGGTCATGT CAGGATTCCT AAGCCAGCTG GAGTGAAGAA AGGGTGGCAG 
AGAGCACTGG CTATAGTGTG TGACTTCAAA CTCTTTCTGT ACGATATTGC TGAAGGAAAA 
GCATCTCAGC CCAGTGTTGT CATTAGTCAA GTGATTGACA TGAGGGATGA AGAATTTTCT 
GTGAGTTCAG TCTTGGCTTC TGATGTTATC CATGCAAGTC GGAAAGATAT ACCCTGTATA 
TTTAGGGTCA CAGCTTCCCA GCTCTCAGCA TCTAATAACA AATGTTCAAT CCTGATGCTA 
GCAGACACTG AGAATGAGAA GAATAAGTGG GTGGGAGTGC TGAGTGAATT GCACAAGATT 
TTGAAGAAAA ACAAATTCAG AGACCGCTCA GTCTATGTTC CCAAAGAGGC TTATGACAGC 
ACTCTACCCC TCATTAAAAC AACCCAGGCA GCCGCAATCA TAGATCATGA AAGAATTGCT 
TTGGGAAACG AAGAAGGGTT ATTTGTTGTA CATGTCACCA AAGATGAAAT TATTAGAGTT 
GGTGACAATA AGAAGATTCA TCAGATTGAA CTCATTCCAA ATGATCAGCT TGTTGCTGTG 
ATCTCAGGAC GAAATCGTCA TGTACGACTT TTTCCTATGT CAGCATTGGA TGGGCGAGAG 
ACCGATTTTT ACAAGCTGTC AGAAACTAAA GGGTGTCAAA CCGTAACTTC TGGAAAGGTG 
CGCCATGGAG CTCTCACATG CCTGTGTGTG GCTATGAAAA GGCAGGTCCT CTGTTATGAA 
CTATTTCAGA GCAAGACCCG TCACAGAAAA TTTAAAGAAA TTCAAGTCCC ATATAATGTC 
CAGTGGATGG CAATCTTCAG TGAACAACTC TGTGTGGGAT TCCAGTCAGG ATTTCTAAGA 
TACCCCTTGA ATGGAGAAGG AAATCCATAC AGTATGCTCC ATTCAAATGA CCATACACTA 
TCATTTATTG CACATCAACC AATGGATGCT ATCTGCGCAG TTGAGATCTC CAGTAAAGAA 
TATCTGCTGT GTTTTAACAG CATTGGGATA TACACTGACT GCCAGGGCCG AAGATCTAGA 
CAACAGGAAT TGATGTGGCC AGCAAATCCT TCCTCTTGTT GTTACAATGC ACCATATCTC 
TCGGTGTACA GTGAAAATGC AGTTGATATC TTTGATGTGA ACTCCATGGA ATGGATTCAG 
ACTCTTCCTC TCAAAAAGGT TCGACCCTTA AACAATGAAG GATCATTAAA TCTTTTAGGG 
TTGGAGACCA TTAGATTAAT ATATTTCAAA AATAAGATGG CAGAAGGGGA CGAACTGGTA 
GTACCTGAAA CATCAGATAA TAGTCGGAAA CAAATGGTTA GAAACATTAA CAATAAGCGG 
CGTTATTCCT TCAGAGTCCC AGAAGAGGAA AGGATGCAGC AGAGGAGGGA AATGCTACGA 
GATCCAGAAA TGAGAAATAA ATTAATTTCT AATCCAACTA ATTTTAATCA CATAGCACAC 
ATGGGTCCTG GAGATGGAAT ACAGATCCTG AAAGATCTGC CCATGCCAGG TTTCCCTTAT 
CCATCCCCTC ATCATCACTC CGGTCTGATC TCCTCTCCGA TCAACTTTGA ACACATCTAT 
CACATGACTG TTAATTCTGC TGAAAAATTT CTCTCTCCTG ATTCCATAAA CCCTGAATAT 
TCCCCGTCTC TGCGCTCTGT CCCTGGTACA CCAAGCTTTA TGACTCTGAG GAACCCTCGG 
CCTCAGGAAA GTCGGACAGT ATTCAGTGGC TCAGTCAGTA TTCCATCTAT CACCAAATCC 
CGCCCTGAGC CAGGCCGCTC CATGAGTGCT AGCAGTGGCT TGTCAGCAAG GTCATCCGCA 
CAGAATGGCA GCGCATTAAA GAGGGAATTC TCTGGAGGAA GCTACAGTGC CAAGCGGCAG 
CCCATGCCCT CCCCGTCAGA GGGCTCTTTG TCCTCTGGAG GCATGGACCA AGGAAGTGAT 
GCCCCAGCGA GGGACTTTGA CGGAGAGGAC TCTGACTCTC CGAGGCATTC CACAGCTTCC 
AACAGTTCCA ACCTAAGCAG CCCCCCAAGC CCAGCTTCAC CCCGAAAAAC CAAGAGCCTC 
TCCCTGGAGA GCACTGACCG CGGGAGCTGG GACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858070.1
CDS	1781..6919
Translation	MKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETDPVENTYVWNPSVKFHIQSRSTSPSTSSEAEPVKTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_017002581.1
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002581.1

ORF Insert Sequence:

ATGAAAATAT TGAATAAATG GGAAATGCTG AAAAGAGCTG AGACAGCATG TTTTCGTGAA 
GAAAGGGATG TATTAGTGAA TGGAGACAAT AAATGGATTA CAACCTTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTGCTTACT 
CTACTCAGCA AATTTGAAGA TAGATTGCCT GAAGATATGG CTAGATTTTA CTTGGCTGAG 
ATGGTGATAG CAATTGACTC AGTTCATCAG CTACATTATG TACACAGAGA CATTAAACCT 
GACAATATAC TGATGGATAT GAATGGACAT ATTCGGTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTTCAGTCC TCAGTGGCTG TAGGAACTCC AGATTATATC 
TCTCCTGAAA TCCTTCAAGC CATGGAAGAT GGAAAAGGGA GATATGGACC TGAATGTGAC 
TGGTGGTCTT TGGGGGTCTG TATGTATGAA ATGCTTTACG GAGAAACACC ATTTTATGCA 
GAATCGCTGG TGGAGACATA CGGAAAAATC ATGAACCACA AAGAGAGGTT TCAGTTTCCA 
GCCCAAGTGA CTGATGTGTC TGAAAATGCT AAGGATCTTA TTCGAAGGCT CATTTGTAGC 
AGAGAACATC GACTTGGTCA AAATGGAATA GAAGACTTTA AGAAACACCC ATTTTTCAGT 
GGAATTGATT GGGATAATAT TCGGAACTGT GAAGCACCTT ATATTCCAGA AGTTAGTAGC 
CCAACAGATA CATCGAATTT TGATGTAGAT GATGATTGTT TAAAAAATTC TGAAACGATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CACCATCTGC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTACTTTC TGATCGGAGC TGTTTAAGAG TTACGGCTGG TCCCACCTCA 
CTGGATCTTG ATGTTAATGT TCAGAGGACT CTAGACAACA ACTTAGCAAC TGAAGCTTAT 
GAAAGAAGAA TTAAGCGCCT TGAGCAAGAA AAACTTGAAC TCAGTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTCCAAGC TCTGCAGTAT TCAACTGTTG ATGGTCCACT AACAGCAAGC 
AAAGATTTAG AAATAAAAAA CTTAAAAGAA GAAATTGAAA AACTAAGAAA ACAAGTAACA 
GAATCAAGTC ATTTGGAACA GCAACTTGAA GAAGCTAATG CTGTGAGGCA AGAACTAGAT 
GATGCTTTTA GACAAATCAA GGCTTATGAA AAACAAATCA AAACGTTACA ACAAGAAAGA 
GAAGATCTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGACG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGGCTAACAG AATTGCACAC CCAAAAACAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGTT GAAAGCTTAA GGCAAGAACT GCGCAGAACA 
GAAAGAGCCA AAAAAGAGCT GGAAGTTCAT ACAGAAGCTC TAGCTGCTGA AGCATCTAAA 
GACAGGAAGC TACGTGAACA GAGTGAGCAC TATTCTAAGC AACTGGAAAA TGAATTGGAG 
GGACTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAGTAT GCAGCATAGA ACATCAGCAA 
GAGATAACCA AACTAAAGAC TGATTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAATTA 
TCTAAAAGAG AAGGAATACA TGCAAATGAA ATAAAAAATC TTAAGAAAGA ACTGCATGAT 
TCAGAAGGTC AGCAACTTGC TCTCAACAAA GAAATTATGA TTTTAAAAGA CAAATTGGAA 
AAAACCAGAA GAGAAAGTCA AAGTGAAAGG GAGGAATTTG AAAGTGAGTT CAAACAACAA 
TATGAACGAG AAAAAGTGTT GTTAACTGAA GAAAATAAAA AGCTGACGAG TGAACTTGAT 
AAGCTTACTA CTTTGTATGA GAACTTAAGT ATACACAACC AGCAGTTAGA AGAAGAGGTT 
AAAGATCTAG CAGACAAGAA AGAATCAGTT GCACATTGGG AAGCCCAAAT CACAGAAATA 
ATTCAGTGGG TCAGCGATGA AAAGGATGCA CGAGGGTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AATTGGAGGC ATTAAGAAAT TCCAGCTTGG GTACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCGAAACTG GATATGTCAG CTAGACTGGA GTTGCAGTCG 
GCTCTGGATG CAGAAATAAG AGCCAAACAG GCCATCCAAG AAGAGTTGAA TAAAGTTAAA 
GCATCTAATA TCATAACAGA ATGTAAACTA AAAGATTCAG AGAAGAAGAA CTTGGAACTA 
CTCTCAGAAA TCGAACAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GATCAATTTG AAACTGATCC CGTTGAGAAC ACATATGTAT GGAACCCGAG CGTCAAGTTT 
CACATCCAGT CACGGTCCAC ATCTCCTTCC ACATCTAGTG AAGCTGAGCC AGTTAAGACT 
GTAGACTCCA CTCCACTTTC AGTTCACACA CCAACCTTAA GGAAAAAAGG ATGTCCTGGT 
TCAACTGGCT TTCCACCTAA GCGCAAGACT CACCAGTTTT TTGTAAAATC TTTTACTACT 
CCTACCAAGT GTCATCAGTG TACCTCCTTG ATGGTGGGTT TAATAAGACA GGGCTGTTCA 
TGTGAAGTGT GTGGATTCTC ATGCCATATA ACTTGTGTAA ACAAAGCTCC AACCACTTGT 
CCAGTTCCTC CTGAACAGAC AAAAGGTCCC CTGGGTATAG ATCCTCAGAA AGGAATAGGA 
ACAGCATATG AAGGTCATGT CAGGATTCCT AAGCCAGCTG GAGTGAAGAA AGGGTGGCAG 
AGAGCACTGG CTATAGTGTG TGACTTCAAA CTCTTTCTGT ACGATATTGC TGAAGGAAAA 
GCATCTCAGC CCAGTGTTGT CATTAGTCAA GTGATTGACA TGAGGGATGA AGAATTTTCT 
GTGAGTTCAG TCTTGGCTTC TGATGTTATC CATGCAAGTC GGAAAGATAT ACCCTGTATA 
TTTAGGGTCA CAGCTTCCCA GCTCTCAGCA TCTAATAACA AATGTTCAAT CCTGATGCTA 
GCAGACACTG AGAATGAGAA GAATAAGTGG GTGGGAGTGC TGAGTGAATT GCACAAGATT 
TTGAAGAAAA ACAAATTCAG AGACCGCTCA GTCTATGTTC CCAAAGAGGC TTATGACAGC 
ACTCTACCCC TCATTAAAAC AACCCAGGCA GCCGCAATCA TAGATCATGA AAGAATTGCT 
TTGGGAAACG AAGAAGGGTT ATTTGTTGTA CATGTCACCA AAGATGAAAT TATTAGAGTT 
GGTGACAATA AGAAGATTCA TCAGATTGAA CTCATTCCAA ATGATCAGCT TGTTGCTGTG 
ATCTCAGGAC GAAATCGTCA TGTACGACTT TTTCCTATGT CAGCATTGGA TGGGCGAGAG 
ACCGATTTTT ACAAGCTGTC AGAAACTAAA GGGTGTCAAA CCGTAACTTC TGGAAAGGTG 
CGCCATGGAG CTCTCACATG CCTGTGTGTG GCTATGAAAA GGCAGGTCCT CTGTTATGAA 
CTATTTCAGA GCAAGACCCG TCACAGAAAA TTTAAAGAAA TTCAAGTCCC ATATAATGTC 
CAGTGGATGG CAATCTTCAG TGAACAACTC TGTGTGGGAT TCCAGTCAGG ATTTCTAAGA 
TACCCCTTGA ATGGAGAAGG AAATCCATAC AGTATGCTCC ATTCAAATGA CCATACACTA 
TCATTTATTG CACATCAACC AATGGATGCT ATCTGCGCAG TTGAGATCTC CAGTAAAGAA 
TATCTGCTGT GTTTTAACAG CATTGGGATA TACACTGACT GCCAGGGCCG AAGATCTAGA 
CAACAGGAAT TGATGTGGCC AGCAAATCCT TCCTCTTGTT GTTACAATGC ACCATATCTC 
TCGGTGTACA GTGAAAATGC AGTTGATATC TTTGATGTGA ACTCCATGGA ATGGATTCAG 
ACTCTTCCTC TCAAAAAGGT TCGACCCTTA AACAATGAAG GATCATTAAA TCTTTTAGGG 
TTGGAGACCA TTAGATTAAT ATATTTCAAA AATAAGATGG CAGAAGGGGA CGAACTGGTA 
GTACCTGAAA CATCAGATAA TAGTCGGAAA CAAATGGTTA GAAACATTAA CAATAAGCGG 
CGTTATTCCT TCAGAGTCCC AGAAGAGGAA AGGATGCAGC AGAGGAGGGA AATGCTACGA 
GATCCAGAAA TGAGAAATAA ATTAATTTCT AATCCAACTA ATTTTAATCA CATAGCACAC 
ATGGGTCCTG GAGATGGAAT ACAGATCCTG AAAGATCTGC CCATGCCAGG TTTCCCTTAT 
CCATCCCCTC ATCATCACTC CGGTCTGATC TCCTCTCCGA TCAACTTTGA ACACATCTAT 
CACATGACTG TTAATTCTGC TGAAAAATTT CTCTCTCCTG ATTCCATAAA CCCTGAATAT 
TCCCCGTCTC TGCGCTCTGT CCCTGGTACA CCAAGCTTTA TGACTCTGAG GAACCCTCGG 
CCTCAGGAAA GTCGGACAGT ATTCAGTGGC TCAGTCAGTA TTCCATCTAT CACCAAATCC 
CGCCCTGAGC CAGGCCGCTC CATGAGTGCT AGCAGTGGCT TGTCAGCAAG GTCATCCGCA 
CAGAATGGCA GCGCATTAAA GAGGGAATTC TCTGGAGGAA GCTACAGTGC CAAGCGGCAG 
CCCATGCCCT CCCCGTCAGA GGGCTCTTTG TCCTCTGGAG GCATGGACCA AGGAAGTGAT 
GCCCCAGCGA GGGACTTTGA CGGAGAGGAC TCTGACTCTC CGAGGCATTC CACAGCTTCC 
AACAGTTCCA ACCTAAGCAG CCCCCCAAGC CCAGCTTCAC CCCGAAAAAC CAAGAGCCTC 
TCCCTGGAGA GCACTGACCG CGGGAGCTGG GACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_017002581.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858070.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X16, mRNA.

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CloneID	OHu90670
Clone ID Related Accession (Same CDS sequence)	XM_017002581.1 , XM_017002581.2
Accession Version	XM_017002581.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017002581.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATAT TGAATAAATG GGAAATGCTG AAAAGAGCTG AGACAGCATG TTTTCGTGAA 
GAAAGGGATG TATTAGTGAA TGGAGACAAT AAATGGATTA CAACCTTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTGCTTACT 
CTACTCAGCA AATTTGAAGA TAGATTGCCT GAAGATATGG CTAGATTTTA CTTGGCTGAG 
ATGGTGATAG CAATTGACTC AGTTCATCAG CTACATTATG TACACAGAGA CATTAAACCT 
GACAATATAC TGATGGATAT GAATGGACAT ATTCGGTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTTCAGTCC TCAGTGGCTG TAGGAACTCC AGATTATATC 
TCTCCTGAAA TCCTTCAAGC CATGGAAGAT GGAAAAGGGA GATATGGACC TGAATGTGAC 
TGGTGGTCTT TGGGGGTCTG TATGTATGAA ATGCTTTACG GAGAAACACC ATTTTATGCA 
GAATCGCTGG TGGAGACATA CGGAAAAATC ATGAACCACA AAGAGAGGTT TCAGTTTCCA 
GCCCAAGTGA CTGATGTGTC TGAAAATGCT AAGGATCTTA TTCGAAGGCT CATTTGTAGC 
AGAGAACATC GACTTGGTCA AAATGGAATA GAAGACTTTA AGAAACACCC ATTTTTCAGT 
GGAATTGATT GGGATAATAT TCGGAACTGT GAAGCACCTT ATATTCCAGA AGTTAGTAGC 
CCAACAGATA CATCGAATTT TGATGTAGAT GATGATTGTT TAAAAAATTC TGAAACGATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CACCATCTGC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTACTTTC TGATCGGAGC TGTTTAAGAG TTACGGCTGG TCCCACCTCA 
CTGGATCTTG ATGTTAATGT TCAGAGGACT CTAGACAACA ACTTAGCAAC TGAAGCTTAT 
GAAAGAAGAA TTAAGCGCCT TGAGCAAGAA AAACTTGAAC TCAGTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTCCAAGC TCTGCAGTAT TCAACTGTTG ATGGTCCACT AACAGCAAGC 
AAAGATTTAG AAATAAAAAA CTTAAAAGAA GAAATTGAAA AACTAAGAAA ACAAGTAACA 
GAATCAAGTC ATTTGGAACA GCAACTTGAA GAAGCTAATG CTGTGAGGCA AGAACTAGAT 
GATGCTTTTA GACAAATCAA GGCTTATGAA AAACAAATCA AAACGTTACA ACAAGAAAGA 
GAAGATCTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGACG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGGCTAACAG AATTGCACAC CCAAAAACAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGTT GAAAGCTTAA GGCAAGAACT GCGCAGAACA 
GAAAGAGCCA AAAAAGAGCT GGAAGTTCAT ACAGAAGCTC TAGCTGCTGA AGCATCTAAA 
GACAGGAAGC TACGTGAACA GAGTGAGCAC TATTCTAAGC AACTGGAAAA TGAATTGGAG 
GGACTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAGTAT GCAGCATAGA ACATCAGCAA 
GAGATAACCA AACTAAAGAC TGATTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAATTA 
TCTAAAAGAG AAGGAATACA TGCAAATGAA ATAAAAAATC TTAAGAAAGA ACTGCATGAT 
TCAGAAGGTC AGCAACTTGC TCTCAACAAA GAAATTATGA TTTTAAAAGA CAAATTGGAA 
AAAACCAGAA GAGAAAGTCA AAGTGAAAGG GAGGAATTTG AAAGTGAGTT CAAACAACAA 
TATGAACGAG AAAAAGTGTT GTTAACTGAA GAAAATAAAA AGCTGACGAG TGAACTTGAT 
AAGCTTACTA CTTTGTATGA GAACTTAAGT ATACACAACC AGCAGTTAGA AGAAGAGGTT 
AAAGATCTAG CAGACAAGAA AGAATCAGTT GCACATTGGG AAGCCCAAAT CACAGAAATA 
ATTCAGTGGG TCAGCGATGA AAAGGATGCA CGAGGGTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AATTGGAGGC ATTAAGAAAT TCCAGCTTGG GTACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCGAAACTG GATATGTCAG CTAGACTGGA GTTGCAGTCG 
GCTCTGGATG CAGAAATAAG AGCCAAACAG GCCATCCAAG AAGAGTTGAA TAAAGTTAAA 
GCATCTAATA TCATAACAGA ATGTAAACTA AAAGATTCAG AGAAGAAGAA CTTGGAACTA 
CTCTCAGAAA TCGAACAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GATCAATTTG AAACTGATCC CGTTGAGAAC ACATATGTAT GGAACCCGAG CGTCAAGTTT 
CACATCCAGT CACGGTCCAC ATCTCCTTCC ACATCTAGTG AAGCTGAGCC AGTTAAGACT 
GTAGACTCCA CTCCACTTTC AGTTCACACA CCAACCTTAA GGAAAAAAGG ATGTCCTGGT 
TCAACTGGCT TTCCACCTAA GCGCAAGACT CACCAGTTTT TTGTAAAATC TTTTACTACT 
CCTACCAAGT GTCATCAGTG TACCTCCTTG ATGGTGGGTT TAATAAGACA GGGCTGTTCA 
TGTGAAGTGT GTGGATTCTC ATGCCATATA ACTTGTGTAA ACAAAGCTCC AACCACTTGT 
CCAGTTCCTC CTGAACAGAC AAAAGGTCCC CTGGGTATAG ATCCTCAGAA AGGAATAGGA 
ACAGCATATG AAGGTCATGT CAGGATTCCT AAGCCAGCTG GAGTGAAGAA AGGGTGGCAG 
AGAGCACTGG CTATAGTGTG TGACTTCAAA CTCTTTCTGT ACGATATTGC TGAAGGAAAA 
GCATCTCAGC CCAGTGTTGT CATTAGTCAA GTGATTGACA TGAGGGATGA AGAATTTTCT 
GTGAGTTCAG TCTTGGCTTC TGATGTTATC CATGCAAGTC GGAAAGATAT ACCCTGTATA 
TTTAGGGTCA CAGCTTCCCA GCTCTCAGCA TCTAATAACA AATGTTCAAT CCTGATGCTA 
GCAGACACTG AGAATGAGAA GAATAAGTGG GTGGGAGTGC TGAGTGAATT GCACAAGATT 
TTGAAGAAAA ACAAATTCAG AGACCGCTCA GTCTATGTTC CCAAAGAGGC TTATGACAGC 
ACTCTACCCC TCATTAAAAC AACCCAGGCA GCCGCAATCA TAGATCATGA AAGAATTGCT 
TTGGGAAACG AAGAAGGGTT ATTTGTTGTA CATGTCACCA AAGATGAAAT TATTAGAGTT 
GGTGACAATA AGAAGATTCA TCAGATTGAA CTCATTCCAA ATGATCAGCT TGTTGCTGTG 
ATCTCAGGAC GAAATCGTCA TGTACGACTT TTTCCTATGT CAGCATTGGA TGGGCGAGAG 
ACCGATTTTT ACAAGCTGTC AGAAACTAAA GGGTGTCAAA CCGTAACTTC TGGAAAGGTG 
CGCCATGGAG CTCTCACATG CCTGTGTGTG GCTATGAAAA GGCAGGTCCT CTGTTATGAA 
CTATTTCAGA GCAAGACCCG TCACAGAAAA TTTAAAGAAA TTCAAGTCCC ATATAATGTC 
CAGTGGATGG CAATCTTCAG TGAACAACTC TGTGTGGGAT TCCAGTCAGG ATTTCTAAGA 
TACCCCTTGA ATGGAGAAGG AAATCCATAC AGTATGCTCC ATTCAAATGA CCATACACTA 
TCATTTATTG CACATCAACC AATGGATGCT ATCTGCGCAG TTGAGATCTC CAGTAAAGAA 
TATCTGCTGT GTTTTAACAG CATTGGGATA TACACTGACT GCCAGGGCCG AAGATCTAGA 
CAACAGGAAT TGATGTGGCC AGCAAATCCT TCCTCTTGTT GTTACAATGC ACCATATCTC 
TCGGTGTACA GTGAAAATGC AGTTGATATC TTTGATGTGA ACTCCATGGA ATGGATTCAG 
ACTCTTCCTC TCAAAAAGGT TCGACCCTTA AACAATGAAG GATCATTAAA TCTTTTAGGG 
TTGGAGACCA TTAGATTAAT ATATTTCAAA AATAAGATGG CAGAAGGGGA CGAACTGGTA 
GTACCTGAAA CATCAGATAA TAGTCGGAAA CAAATGGTTA GAAACATTAA CAATAAGCGG 
CGTTATTCCT TCAGAGTCCC AGAAGAGGAA AGGATGCAGC AGAGGAGGGA AATGCTACGA 
GATCCAGAAA TGAGAAATAA ATTAATTTCT AATCCAACTA ATTTTAATCA CATAGCACAC 
ATGGGTCCTG GAGATGGAAT ACAGATCCTG AAAGATCTGC CCATGCCAGG TTTCCCTTAT 
CCATCCCCTC ATCATCACTC CGGTCTGATC TCCTCTCCGA TCAACTTTGA ACACATCTAT 
CACATGACTG TTAATTCTGC TGAAAAATTT CTCTCTCCTG ATTCCATAAA CCCTGAATAT 
TCCCCGTCTC TGCGCTCTGT CCCTGGTACA CCAAGCTTTA TGACTCTGAG GAACCCTCGG 
CCTCAGGAAA GTCGGACAGT ATTCAGTGGC TCAGTCAGTA TTCCATCTAT CACCAAATCC 
CGCCCTGAGC CAGGCCGCTC CATGAGTGCT AGCAGTGGCT TGTCAGCAAG GTCATCCGCA 
CAGAATGGCA GCGCATTAAA GAGGGAATTC TCTGGAGGAA GCTACAGTGC CAAGCGGCAG 
CCCATGCCCT CCCCGTCAGA GGGCTCTTTG TCCTCTGGAG GCATGGACCA AGGAAGTGAT 
GCCCCAGCGA GGGACTTTGA CGGAGAGGAC TCTGACTCTC CGAGGCATTC CACAGCTTCC 
AACAGTTCCA ACCTAAGCAG CCCCCCAAGC CCAGCTTCAC CCCGAAAAAC CAAGAGCCTC 
TCCCTGGAGA GCACTGACCG CGGGAGCTGG GACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858070.1
CDS	1791..6929
Translation	MKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETDPVENTYVWNPSVKFHIQSRSTSPSTSSEAEPVKTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_017002581.2
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002581.2

ORF Insert Sequence:

ATGAAAATAT TGAATAAATG GGAAATGCTG AAAAGAGCTG AGACAGCATG TTTTCGTGAA 
GAAAGGGATG TATTAGTGAA TGGAGACAAT AAATGGATTA CAACCTTGCA CTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTGCTTACT 
CTACTCAGCA AATTTGAAGA TAGATTGCCT GAAGATATGG CTAGATTTTA CTTGGCTGAG 
ATGGTGATAG CAATTGACTC AGTTCATCAG CTACATTATG TACACAGAGA CATTAAACCT 
GACAATATAC TGATGGATAT GAATGGACAT ATTCGGTTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTTCAGTCC TCAGTGGCTG TAGGAACTCC AGATTATATC 
TCTCCTGAAA TCCTTCAAGC CATGGAAGAT GGAAAAGGGA GATATGGACC TGAATGTGAC 
TGGTGGTCTT TGGGGGTCTG TATGTATGAA ATGCTTTACG GAGAAACACC ATTTTATGCA 
GAATCGCTGG TGGAGACATA CGGAAAAATC ATGAACCACA AAGAGAGGTT TCAGTTTCCA 
GCCCAAGTGA CTGATGTGTC TGAAAATGCT AAGGATCTTA TTCGAAGGCT CATTTGTAGC 
AGAGAACATC GACTTGGTCA AAATGGAATA GAAGACTTTA AGAAACACCC ATTTTTCAGT 
GGAATTGATT GGGATAATAT TCGGAACTGT GAAGCACCTT ATATTCCAGA AGTTAGTAGC 
CCAACAGATA CATCGAATTT TGATGTAGAT GATGATTGTT TAAAAAATTC TGAAACGATG 
CCCCCACCAA CACATACTGC ATTTTCTGGC CACCATCTGC CATTTGTTGG TTTTACATAT 
ACTAGTAGCT GTGTACTTTC TGATCGGAGC TGTTTAAGAG TTACGGCTGG TCCCACCTCA 
CTGGATCTTG ATGTTAATGT TCAGAGGACT CTAGACAACA ACTTAGCAAC TGAAGCTTAT 
GAAAGAAGAA TTAAGCGCCT TGAGCAAGAA AAACTTGAAC TCAGTAGAAA ACTTCAAGAG 
TCAACACAGA CTGTCCAAGC TCTGCAGTAT TCAACTGTTG ATGGTCCACT AACAGCAAGC 
AAAGATTTAG AAATAAAAAA CTTAAAAGAA GAAATTGAAA AACTAAGAAA ACAAGTAACA 
GAATCAAGTC ATTTGGAACA GCAACTTGAA GAAGCTAATG CTGTGAGGCA AGAACTAGAT 
GATGCTTTTA GACAAATCAA GGCTTATGAA AAACAAATCA AAACGTTACA ACAAGAAAGA 
GAAGATCTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGACG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGGCTAACAG AATTGCACAC CCAAAAACAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGTT GAAAGCTTAA GGCAAGAACT GCGCAGAACA 
GAAAGAGCCA AAAAAGAGCT GGAAGTTCAT ACAGAAGCTC TAGCTGCTGA AGCATCTAAA 
GACAGGAAGC TACGTGAACA GAGTGAGCAC TATTCTAAGC AACTGGAAAA TGAATTGGAG 
GGACTGAAGC AAAAACAAAT TAGTTACTCA CCAGGAGTAT GCAGCATAGA ACATCAGCAA 
GAGATAACCA AACTAAAGAC TGATTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAATTA 
TCTAAAAGAG AAGGAATACA TGCAAATGAA ATAAAAAATC TTAAGAAAGA ACTGCATGAT 
TCAGAAGGTC AGCAACTTGC TCTCAACAAA GAAATTATGA TTTTAAAAGA CAAATTGGAA 
AAAACCAGAA GAGAAAGTCA AAGTGAAAGG GAGGAATTTG AAAGTGAGTT CAAACAACAA 
TATGAACGAG AAAAAGTGTT GTTAACTGAA GAAAATAAAA AGCTGACGAG TGAACTTGAT 
AAGCTTACTA CTTTGTATGA GAACTTAAGT ATACACAACC AGCAGTTAGA AGAAGAGGTT 
AAAGATCTAG CAGACAAGAA AGAATCAGTT GCACATTGGG AAGCCCAAAT CACAGAAATA 
ATTCAGTGGG TCAGCGATGA AAAGGATGCA CGAGGGTATC TTCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AATTGGAGGC ATTAAGAAAT TCCAGCTTGG GTACACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCGAAACTG GATATGTCAG CTAGACTGGA GTTGCAGTCG 
GCTCTGGATG CAGAAATAAG AGCCAAACAG GCCATCCAAG AAGAGTTGAA TAAAGTTAAA 
GCATCTAATA TCATAACAGA ATGTAAACTA AAAGATTCAG AGAAGAAGAA CTTGGAACTA 
CTCTCAGAAA TCGAACAGCT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GATCAATTTG AAACTGATCC CGTTGAGAAC ACATATGTAT GGAACCCGAG CGTCAAGTTT 
CACATCCAGT CACGGTCCAC ATCTCCTTCC ACATCTAGTG AAGCTGAGCC AGTTAAGACT 
GTAGACTCCA CTCCACTTTC AGTTCACACA CCAACCTTAA GGAAAAAAGG ATGTCCTGGT 
TCAACTGGCT TTCCACCTAA GCGCAAGACT CACCAGTTTT TTGTAAAATC TTTTACTACT 
CCTACCAAGT GTCATCAGTG TACCTCCTTG ATGGTGGGTT TAATAAGACA GGGCTGTTCA 
TGTGAAGTGT GTGGATTCTC ATGCCATATA ACTTGTGTAA ACAAAGCTCC AACCACTTGT 
CCAGTTCCTC CTGAACAGAC AAAAGGTCCC CTGGGTATAG ATCCTCAGAA AGGAATAGGA 
ACAGCATATG AAGGTCATGT CAGGATTCCT AAGCCAGCTG GAGTGAAGAA AGGGTGGCAG 
AGAGCACTGG CTATAGTGTG TGACTTCAAA CTCTTTCTGT ACGATATTGC TGAAGGAAAA 
GCATCTCAGC CCAGTGTTGT CATTAGTCAA GTGATTGACA TGAGGGATGA AGAATTTTCT 
GTGAGTTCAG TCTTGGCTTC TGATGTTATC CATGCAAGTC GGAAAGATAT ACCCTGTATA 
TTTAGGGTCA CAGCTTCCCA GCTCTCAGCA TCTAATAACA AATGTTCAAT CCTGATGCTA 
GCAGACACTG AGAATGAGAA GAATAAGTGG GTGGGAGTGC TGAGTGAATT GCACAAGATT 
TTGAAGAAAA ACAAATTCAG AGACCGCTCA GTCTATGTTC CCAAAGAGGC TTATGACAGC 
ACTCTACCCC TCATTAAAAC AACCCAGGCA GCCGCAATCA TAGATCATGA AAGAATTGCT 
TTGGGAAACG AAGAAGGGTT ATTTGTTGTA CATGTCACCA AAGATGAAAT TATTAGAGTT 
GGTGACAATA AGAAGATTCA TCAGATTGAA CTCATTCCAA ATGATCAGCT TGTTGCTGTG 
ATCTCAGGAC GAAATCGTCA TGTACGACTT TTTCCTATGT CAGCATTGGA TGGGCGAGAG 
ACCGATTTTT ACAAGCTGTC AGAAACTAAA GGGTGTCAAA CCGTAACTTC TGGAAAGGTG 
CGCCATGGAG CTCTCACATG CCTGTGTGTG GCTATGAAAA GGCAGGTCCT CTGTTATGAA 
CTATTTCAGA GCAAGACCCG TCACAGAAAA TTTAAAGAAA TTCAAGTCCC ATATAATGTC 
CAGTGGATGG CAATCTTCAG TGAACAACTC TGTGTGGGAT TCCAGTCAGG ATTTCTAAGA 
TACCCCTTGA ATGGAGAAGG AAATCCATAC AGTATGCTCC ATTCAAATGA CCATACACTA 
TCATTTATTG CACATCAACC AATGGATGCT ATCTGCGCAG TTGAGATCTC CAGTAAAGAA 
TATCTGCTGT GTTTTAACAG CATTGGGATA TACACTGACT GCCAGGGCCG AAGATCTAGA 
CAACAGGAAT TGATGTGGCC AGCAAATCCT TCCTCTTGTT GTTACAATGC ACCATATCTC 
TCGGTGTACA GTGAAAATGC AGTTGATATC TTTGATGTGA ACTCCATGGA ATGGATTCAG 
ACTCTTCCTC TCAAAAAGGT TCGACCCTTA AACAATGAAG GATCATTAAA TCTTTTAGGG 
TTGGAGACCA TTAGATTAAT ATATTTCAAA AATAAGATGG CAGAAGGGGA CGAACTGGTA 
GTACCTGAAA CATCAGATAA TAGTCGGAAA CAAATGGTTA GAAACATTAA CAATAAGCGG 
CGTTATTCCT TCAGAGTCCC AGAAGAGGAA AGGATGCAGC AGAGGAGGGA AATGCTACGA 
GATCCAGAAA TGAGAAATAA ATTAATTTCT AATCCAACTA ATTTTAATCA CATAGCACAC 
ATGGGTCCTG GAGATGGAAT ACAGATCCTG AAAGATCTGC CCATGCCAGG TTTCCCTTAT 
CCATCCCCTC ATCATCACTC CGGTCTGATC TCCTCTCCGA TCAACTTTGA ACACATCTAT 
CACATGACTG TTAATTCTGC TGAAAAATTT CTCTCTCCTG ATTCCATAAA CCCTGAATAT 
TCCCCGTCTC TGCGCTCTGT CCCTGGTACA CCAAGCTTTA TGACTCTGAG GAACCCTCGG 
CCTCAGGAAA GTCGGACAGT ATTCAGTGGC TCAGTCAGTA TTCCATCTAT CACCAAATCC 
CGCCCTGAGC CAGGCCGCTC CATGAGTGCT AGCAGTGGCT TGTCAGCAAG GTCATCCGCA 
CAGAATGGCA GCGCATTAAA GAGGGAATTC TCTGGAGGAA GCTACAGTGC CAAGCGGCAG 
CCCATGCCCT CCCCGTCAGA GGGCTCTTTG TCCTCTGGAG GCATGGACCA AGGAAGTGAT 
GCCCCAGCGA GGGACTTTGA CGGAGAGGAC TCTGACTCTC CGAGGCATTC CACAGCTTCC 
AACAGTTCCA ACCTAAGCAG CCCCCCAAGC CCAGCTTCAC CCCGAAAAAC CAAGAGCCTC 
TCCCTGGAGA GCACTGACCG CGGGAGCTGG GACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_017002582.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858071.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OHu90671
Clone ID Related Accession (Same CDS sequence)	XM_017002582.1 , XM_017002582.2
Accession Version	XM_017002582.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCCACTC CACTTTCAGT TCACACACCA 
ACCTTAAGGA AAAAAGGATG TCCTGGTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAC 
CAGTTTTTTG TAAAATCTTT TACTACTCCT ACCAAGTGTC ATCAGTGTAC CTCCTTGATG 
GTGGGTTTAA TAAGACAGGG CTGTTCATGT GAAGTGTGTG GATTCTCATG CCATATAACT 
TGTGTAAACA AAGCTCCAAC CACTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCCCTG 
GGTATAGATC CTCAGAAAGG AATAGGAACA GCATATGAAG GTCATGTCAG GATTCCTAAG 
CCAGCTGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTA TAGTGTGTGA CTTCAAACTC 
TTTCTGTACG ATATTGCTGA AGGAAAAGCA TCTCAGCCCA GTGTTGTCAT TAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATCCAT 
GCAAGTCGGA AAGATATACC CTGTATATTT AGGGTCACAG CTTCCCAGCT CTCAGCATCT 
AATAACAAAT GTTCAATCCT GATGCTAGCA GACACTGAGA ATGAGAAGAA TAAGTGGGTG 
GGAGTGCTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTACCCCTCA TTAAAACAAC CCAGGCAGCC 
GCAATCATAG ATCATGAAAG AATTGCTTTG GGAAACGAAG AAGGGTTATT TGTTGTACAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATGTCAG CATTGGATGG GCGAGAGACC GATTTTTACA AGCTGTCAGA AACTAAAGGG 
TGTCAAACCG TAACTTCTGG AAAGGTGCGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGAAAATTT 
AAAGAAATTC AAGTCCCATA TAATGTCCAG TGGATGGCAA TCTTCAGTGA ACAACTCTGT 
GTGGGATTCC AGTCAGGATT TCTAAGATAC CCCTTGAATG GAGAAGGAAA TCCATACAGT 
ATGCTCCATT CAAATGACCA TACACTATCA TTTATTGCAC ATCAACCAAT GGATGCTATC 
TGCGCAGTTG AGATCTCCAG TAAAGAATAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAATTGA TGTGGCCAGC AAATCCTTCC 
TCTTGTTGTT ACAATGCACC ATATCTCTCG GTGTACAGTG AAAATGCAGT TGATATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTTCCTCTCA AAAAGGTTCG ACCCTTAAAC 
AATGAAGGAT CATTAAATCT TTTAGGGTTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGACGA ACTGGTAGTA CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ACATTAACAA TAAGCGGCGT TATTCCTTCA GAGTCCCAGA AGAGGAAAGG 
ATGCAGCAGA GGAGGGAAAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAT 
CCAACTAATT TTAATCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTGAAA 
GATCTGCCCA TGGACAAGAA GTTGGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858071.1
CDS	1056..5825
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMDKKLV

Target ORF information:

RefSeq Version	XM_017002582.1
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002582.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCCACTC CACTTTCAGT TCACACACCA 
ACCTTAAGGA AAAAAGGATG TCCTGGTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAC 
CAGTTTTTTG TAAAATCTTT TACTACTCCT ACCAAGTGTC ATCAGTGTAC CTCCTTGATG 
GTGGGTTTAA TAAGACAGGG CTGTTCATGT GAAGTGTGTG GATTCTCATG CCATATAACT 
TGTGTAAACA AAGCTCCAAC CACTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCCCTG 
GGTATAGATC CTCAGAAAGG AATAGGAACA GCATATGAAG GTCATGTCAG GATTCCTAAG 
CCAGCTGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTA TAGTGTGTGA CTTCAAACTC 
TTTCTGTACG ATATTGCTGA AGGAAAAGCA TCTCAGCCCA GTGTTGTCAT TAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATCCAT 
GCAAGTCGGA AAGATATACC CTGTATATTT AGGGTCACAG CTTCCCAGCT CTCAGCATCT 
AATAACAAAT GTTCAATCCT GATGCTAGCA GACACTGAGA ATGAGAAGAA TAAGTGGGTG 
GGAGTGCTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTACCCCTCA TTAAAACAAC CCAGGCAGCC 
GCAATCATAG ATCATGAAAG AATTGCTTTG GGAAACGAAG AAGGGTTATT TGTTGTACAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATGTCAG CATTGGATGG GCGAGAGACC GATTTTTACA AGCTGTCAGA AACTAAAGGG 
TGTCAAACCG TAACTTCTGG AAAGGTGCGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGAAAATTT 
AAAGAAATTC AAGTCCCATA TAATGTCCAG TGGATGGCAA TCTTCAGTGA ACAACTCTGT 
GTGGGATTCC AGTCAGGATT TCTAAGATAC CCCTTGAATG GAGAAGGAAA TCCATACAGT 
ATGCTCCATT CAAATGACCA TACACTATCA TTTATTGCAC ATCAACCAAT GGATGCTATC 
TGCGCAGTTG AGATCTCCAG TAAAGAATAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAATTGA TGTGGCCAGC AAATCCTTCC 
TCTTGTTGTT ACAATGCACC ATATCTCTCG GTGTACAGTG AAAATGCAGT TGATATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTTCCTCTCA AAAAGGTTCG ACCCTTAAAC 
AATGAAGGAT CATTAAATCT TTTAGGGTTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGACGA ACTGGTAGTA CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ACATTAACAA TAAGCGGCGT TATTCCTTCA GAGTCCCAGA AGAGGAAAGG 
ATGCAGCAGA GGAGGGAAAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAT 
CCAACTAATT TTAATCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTGAAA 
GATCTGCCCA TGGACAAGAA GTTGGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_017002582.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858071.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OHu90671
Clone ID Related Accession (Same CDS sequence)	XM_017002582.1 , XM_017002582.2
Accession Version	XM_017002582.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017002582.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCCACTC CACTTTCAGT TCACACACCA 
ACCTTAAGGA AAAAAGGATG TCCTGGTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAC 
CAGTTTTTTG TAAAATCTTT TACTACTCCT ACCAAGTGTC ATCAGTGTAC CTCCTTGATG 
GTGGGTTTAA TAAGACAGGG CTGTTCATGT GAAGTGTGTG GATTCTCATG CCATATAACT 
TGTGTAAACA AAGCTCCAAC CACTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCCCTG 
GGTATAGATC CTCAGAAAGG AATAGGAACA GCATATGAAG GTCATGTCAG GATTCCTAAG 
CCAGCTGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTA TAGTGTGTGA CTTCAAACTC 
TTTCTGTACG ATATTGCTGA AGGAAAAGCA TCTCAGCCCA GTGTTGTCAT TAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATCCAT 
GCAAGTCGGA AAGATATACC CTGTATATTT AGGGTCACAG CTTCCCAGCT CTCAGCATCT 
AATAACAAAT GTTCAATCCT GATGCTAGCA GACACTGAGA ATGAGAAGAA TAAGTGGGTG 
GGAGTGCTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTACCCCTCA TTAAAACAAC CCAGGCAGCC 
GCAATCATAG ATCATGAAAG AATTGCTTTG GGAAACGAAG AAGGGTTATT TGTTGTACAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATGTCAG CATTGGATGG GCGAGAGACC GATTTTTACA AGCTGTCAGA AACTAAAGGG 
TGTCAAACCG TAACTTCTGG AAAGGTGCGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGAAAATTT 
AAAGAAATTC AAGTCCCATA TAATGTCCAG TGGATGGCAA TCTTCAGTGA ACAACTCTGT 
GTGGGATTCC AGTCAGGATT TCTAAGATAC CCCTTGAATG GAGAAGGAAA TCCATACAGT 
ATGCTCCATT CAAATGACCA TACACTATCA TTTATTGCAC ATCAACCAAT GGATGCTATC 
TGCGCAGTTG AGATCTCCAG TAAAGAATAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAATTGA TGTGGCCAGC AAATCCTTCC 
TCTTGTTGTT ACAATGCACC ATATCTCTCG GTGTACAGTG AAAATGCAGT TGATATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTTCCTCTCA AAAAGGTTCG ACCCTTAAAC 
AATGAAGGAT CATTAAATCT TTTAGGGTTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGACGA ACTGGTAGTA CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ACATTAACAA TAAGCGGCGT TATTCCTTCA GAGTCCCAGA AGAGGAAAGG 
ATGCAGCAGA GGAGGGAAAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAT 
CCAACTAATT TTAATCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTGAAA 
GATCTGCCCA TGGACAAGAA GTTGGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858071.1
CDS	969..5738
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMDKKLV

Target ORF information:

RefSeq Version	XM_017002582.2
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002582.2

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCCACTC CACTTTCAGT TCACACACCA 
ACCTTAAGGA AAAAAGGATG TCCTGGTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAC 
CAGTTTTTTG TAAAATCTTT TACTACTCCT ACCAAGTGTC ATCAGTGTAC CTCCTTGATG 
GTGGGTTTAA TAAGACAGGG CTGTTCATGT GAAGTGTGTG GATTCTCATG CCATATAACT 
TGTGTAAACA AAGCTCCAAC CACTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCCCTG 
GGTATAGATC CTCAGAAAGG AATAGGAACA GCATATGAAG GTCATGTCAG GATTCCTAAG 
CCAGCTGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTA TAGTGTGTGA CTTCAAACTC 
TTTCTGTACG ATATTGCTGA AGGAAAAGCA TCTCAGCCCA GTGTTGTCAT TAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATCCAT 
GCAAGTCGGA AAGATATACC CTGTATATTT AGGGTCACAG CTTCCCAGCT CTCAGCATCT 
AATAACAAAT GTTCAATCCT GATGCTAGCA GACACTGAGA ATGAGAAGAA TAAGTGGGTG 
GGAGTGCTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTACCCCTCA TTAAAACAAC CCAGGCAGCC 
GCAATCATAG ATCATGAAAG AATTGCTTTG GGAAACGAAG AAGGGTTATT TGTTGTACAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATGTCAG CATTGGATGG GCGAGAGACC GATTTTTACA AGCTGTCAGA AACTAAAGGG 
TGTCAAACCG TAACTTCTGG AAAGGTGCGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGAAAATTT 
AAAGAAATTC AAGTCCCATA TAATGTCCAG TGGATGGCAA TCTTCAGTGA ACAACTCTGT 
GTGGGATTCC AGTCAGGATT TCTAAGATAC CCCTTGAATG GAGAAGGAAA TCCATACAGT 
ATGCTCCATT CAAATGACCA TACACTATCA TTTATTGCAC ATCAACCAAT GGATGCTATC 
TGCGCAGTTG AGATCTCCAG TAAAGAATAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAATTGA TGTGGCCAGC AAATCCTTCC 
TCTTGTTGTT ACAATGCACC ATATCTCTCG GTGTACAGTG AAAATGCAGT TGATATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTTCCTCTCA AAAAGGTTCG ACCCTTAAAC 
AATGAAGGAT CATTAAATCT TTTAGGGTTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGACGA ACTGGTAGTA CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ACATTAACAA TAAGCGGCGT TATTCCTTCA GAGTCCCAGA AGAGGAAAGG 
ATGCAGCAGA GGAGGGAAAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAT 
CCAACTAATT TTAATCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTGAAA 
GATCTGCCCA TGGACAAGAA GTTGGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( NM_003607.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003598.2 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant B, mRNA.

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CloneID	OHu03424
Clone ID Related Accession (Same CDS sequence)	NM_003607.3 , NM_003607.4
Accession Version	NM_003607.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL627308.20, BQ232716.1, AJ518975.1, BX648219.1, BF765241.1, BC050335.1 and AL353689.26. On Aug 25, 2006 this sequence version replaced NM_003607.2. Transcript Variant: This variant (B) encodes the longer isoform (B). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ518975.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCCACTC CACTTTCAGT TCACACACCA 
ACCTTAAGGA AAAAAGGATG TCCTGGTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAC 
CAGTTTTTTG TAAAATCTTT TACTACTCCT ACCAAGTGTC ATCAGTGTAC CTCCTTGATG 
GTGGGTTTAA TAAGACAGGG CTGTTCATGT GAAGTGTGTG GATTCTCATG CCATATAACT 
TGTGTAAACA AAGCTCCAAC CACTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCCCTG 
GGTATAGATC CTCAGAAAGG AATAGGAACA GCATATGAAG GTCATGTCAG GATTCCTAAG 
CCAGCTGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTA TAGTGTGTGA CTTCAAACTC 
TTTCTGTACG ATATTGCTGA AGGAAAAGCA TCTCAGCCCA GTGTTGTCAT TAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATCCAT 
GCAAGTCGGA AAGATATACC CTGTATATTT AGGGTCACAG CTTCCCAGCT CTCAGCATCT 
AATAACAAAT GTTCAATCCT GATGCTAGCA GACACTGAGA ATGAGAAGAA TAAGTGGGTG 
GGAGTGCTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTACCCCTCA TTAAAACAAC CCAGGCAGCC 
GCAATCATAG ATCATGAAAG AATTGCTTTG GGAAACGAAG AAGGGTTATT TGTTGTACAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATGTCAG CATTGGATGG GCGAGAGACC GATTTTTACA AGCTGTCAGA AACTAAAGGG 
TGTCAAACCG TAACTTCTGG AAAGGTGCGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGAAAATTT 
AAAGAAATTC AAGTCCCATA TAATGTCCAG TGGATGGCAA TCTTCAGTGA ACAACTCTGT 
GTGGGATTCC AGTCAGGATT TCTAAGATAC CCCTTGAATG GAGAAGGAAA TCCATACAGT 
ATGCTCCATT CAAATGACCA TACACTATCA TTTATTGCAC ATCAACCAAT GGATGCTATC 
TGCGCAGTTG AGATCTCCAG TAAAGAATAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAATTGA TGTGGCCAGC AAATCCTTCC 
TCTTGTTGTT ACAATGCACC ATATCTCTCG GTGTACAGTG AAAATGCAGT TGATATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTTCCTCTCA AAAAGGTTCG ACCCTTAAAC 
AATGAAGGAT CATTAAATCT TTTAGGGTTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGACGA ACTGGTAGTA CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ACATTAACAA TAAGCGGCGT TATTCCTTCA GAGTCCCAGA AGAGGAAAGG 
ATGCAGCAGA GGAGGGAAAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAT 
CCAACTAATT TTAATCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTGAAA 
GATCTGCCCA TGAACCCTCG GCCTCAGGAA AGTCGGACAG TATTCAGTGG CTCAGTCAGT 
ATTCCATCTA TCACCAAATC CCGCCCTGAG CCAGGCCGCT CCATGAGTGC TAGCAGTGGC 
TTGTCAGCAA GGTCATCCGC ACAGAATGGC AGCGCATTAA AGAGGGAATT CTCTGGAGGA 
AGCTACAGTG CCAAGCGGCA GCCCATGCCC TCCCCGTCAG AGGGCTCTTT GTCCTCTGGA 
GGCATGGACC AAGGAAGTGA TGCCCCAGCG AGGGACTTTG ACGGAGAGGA CTCTGACTCT 
CCGAGGCATT CCACAGCTTC CAACAGTTCC AACCTAAGCA GCCCCCCAAG CCCAGCTTCA 
CCCCGAAAAA CCAAGAGCCT CTCCCTGGAG AGCACTGACC GCGGGAGCTG GGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003598.2
CDS	944..6103
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	NM_003607.3
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003607.3

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCCACTC CACTTTCAGT TCACACACCA 
ACCTTAAGGA AAAAAGGATG TCCTGGTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAC 
CAGTTTTTTG TAAAATCTTT TACTACTCCT ACCAAGTGTC ATCAGTGTAC CTCCTTGATG 
GTGGGTTTAA TAAGACAGGG CTGTTCATGT GAAGTGTGTG GATTCTCATG CCATATAACT 
TGTGTAAACA AAGCTCCAAC CACTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCCCTG 
GGTATAGATC CTCAGAAAGG AATAGGAACA GCATATGAAG GTCATGTCAG GATTCCTAAG 
CCAGCTGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTA TAGTGTGTGA CTTCAAACTC 
TTTCTGTACG ATATTGCTGA AGGAAAAGCA TCTCAGCCCA GTGTTGTCAT TAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATCCAT 
GCAAGTCGGA AAGATATACC CTGTATATTT AGGGTCACAG CTTCCCAGCT CTCAGCATCT 
AATAACAAAT GTTCAATCCT GATGCTAGCA GACACTGAGA ATGAGAAGAA TAAGTGGGTG 
GGAGTGCTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTACCCCTCA TTAAAACAAC CCAGGCAGCC 
GCAATCATAG ATCATGAAAG AATTGCTTTG GGAAACGAAG AAGGGTTATT TGTTGTACAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATGTCAG CATTGGATGG GCGAGAGACC GATTTTTACA AGCTGTCAGA AACTAAAGGG 
TGTCAAACCG TAACTTCTGG AAAGGTGCGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGAAAATTT 
AAAGAAATTC AAGTCCCATA TAATGTCCAG TGGATGGCAA TCTTCAGTGA ACAACTCTGT 
GTGGGATTCC AGTCAGGATT TCTAAGATAC CCCTTGAATG GAGAAGGAAA TCCATACAGT 
ATGCTCCATT CAAATGACCA TACACTATCA TTTATTGCAC ATCAACCAAT GGATGCTATC 
TGCGCAGTTG AGATCTCCAG TAAAGAATAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAATTGA TGTGGCCAGC AAATCCTTCC 
TCTTGTTGTT ACAATGCACC ATATCTCTCG GTGTACAGTG AAAATGCAGT TGATATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTTCCTCTCA AAAAGGTTCG ACCCTTAAAC 
AATGAAGGAT CATTAAATCT TTTAGGGTTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGACGA ACTGGTAGTA CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ACATTAACAA TAAGCGGCGT TATTCCTTCA GAGTCCCAGA AGAGGAAAGG 
ATGCAGCAGA GGAGGGAAAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAT 
CCAACTAATT TTAATCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTGAAA 
GATCTGCCCA TGAACCCTCG GCCTCAGGAA AGTCGGACAG TATTCAGTGG CTCAGTCAGT 
ATTCCATCTA TCACCAAATC CCGCCCTGAG CCAGGCCGCT CCATGAGTGC TAGCAGTGGC 
TTGTCAGCAA GGTCATCCGC ACAGAATGGC AGCGCATTAA AGAGGGAATT CTCTGGAGGA 
AGCTACAGTG CCAAGCGGCA GCCCATGCCC TCCCCGTCAG AGGGCTCTTT GTCCTCTGGA 
GGCATGGACC AAGGAAGTGA TGCCCCAGCG AGGGACTTTG ACGGAGAGGA CTCTGACTCT 
CCGAGGCATT CCACAGCTTC CAACAGTTCC AACCTAAGCA GCCCCCCAAG CCCAGCTTCA 
CCCCGAAAAA CCAAGAGCCT CTCCCTGGAG AGCACTGACC GCGGGAGCTG GGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( NM_014826.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055641.3 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant A, mRNA.

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CloneID	OHu06683
Clone ID Related Accession (Same CDS sequence)	NM_014826.4 , NM_014826.5
Accession Version	NM_014826.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-03
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL627308.20, BQ232716.1, AJ518976.1, BX648219.1, BF765241.1, BC050335.1 and AL353689.26. On Aug 25, 2006 this sequence version replaced NM_014826.3. Transcript Variant: This variant (A) lacks a coding segment compared to variant B, resulting in an isoform (A) that lacks an internal region, as compared to isoform B. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ518976.1, AY166586.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGCTG GAAGTTCATA CAGAAGCTCT AGCTGCTGAA 
GCATCTAAAG ACAGGAAGCT ACGTGAACAG AGTGAGCACT ATTCTAAGCA ACTGGAAAAT 
GAATTGGAGG GACTGAAGCA AAAACAAATT AGTTACTCAC CAGGAGTATG CAGCATAGAA 
CATCAGCAAG AGATAACCAA ACTAAAGACT GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAATTAT CTAAAAGAGA AGGAATACAT GCAAATGAAA TAAAAAATCT TAAGAAAGAA 
CTGCATGATT CAGAAGGTCA GCAACTTGCT CTCAACAAAG AAATTATGAT TTTAAAAGAC 
AAATTGGAAA AAACCAGAAG AGAAAGTCAA AGTGAAAGGG AGGAATTTGA AAGTGAGTTC 
AAACAACAAT ATGAACGAGA AAAAGTGTTG TTAACTGAAG AAAATAAAAA GCTGACGAGT 
GAACTTGATA AGCTTACTAC TTTGTATGAG AACTTAAGTA TACACAACCA GCAGTTAGAA 
GAAGAGGTTA AAGATCTAGC AGACAAGAAA GAATCAGTTG CACATTGGGA AGCCCAAATC 
ACAGAAATAA TTCAGTGGGT CAGCGATGAA AAGGATGCAC GAGGGTATCT TCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAGGCA TTAAGAAATT CCAGCTTGGG TACACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCGAAACTGG ATATGTCAGC TAGACTGGAG 
TTGCAGTCGG CTCTGGATGC AGAAATAAGA GCCAAACAGG CCATCCAAGA AGAGTTGAAT 
AAAGTTAAAG CATCTAATAT CATAACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAACTAC TCTCAGAAAT CGAACAGCTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AACTGTAGAC TCCACTCCAC TTTCAGTTCA CACACCAACC 
TTAAGGAAAA AAGGATGTCC TGGTTCAACT GGCTTTCCAC CTAAGCGCAA GACTCACCAG 
TTTTTTGTAA AATCTTTTAC TACTCCTACC AAGTGTCATC AGTGTACCTC CTTGATGGTG 
GGTTTAATAA GACAGGGCTG TTCATGTGAA GTGTGTGGAT TCTCATGCCA TATAACTTGT 
GTAAACAAAG CTCCAACCAC TTGTCCAGTT CCTCCTGAAC AGACAAAAGG TCCCCTGGGT 
ATAGATCCTC AGAAAGGAAT AGGAACAGCA TATGAAGGTC ATGTCAGGAT TCCTAAGCCA 
GCTGGAGTGA AGAAAGGGTG GCAGAGAGCA CTGGCTATAG TGTGTGACTT CAAACTCTTT 
CTGTACGATA TTGCTGAAGG AAAAGCATCT CAGCCCAGTG TTGTCATTAG TCAAGTGATT 
GACATGAGGG ATGAAGAATT TTCTGTGAGT TCAGTCTTGG CTTCTGATGT TATCCATGCA 
AGTCGGAAAG ATATACCCTG TATATTTAGG GTCACAGCTT CCCAGCTCTC AGCATCTAAT 
AACAAATGTT CAATCCTGAT GCTAGCAGAC ACTGAGAATG AGAAGAATAA GTGGGTGGGA 
GTGCTGAGTG AATTGCACAA GATTTTGAAG AAAAACAAAT TCAGAGACCG CTCAGTCTAT 
GTTCCCAAAG AGGCTTATGA CAGCACTCTA CCCCTCATTA AAACAACCCA GGCAGCCGCA 
ATCATAGATC ATGAAAGAAT TGCTTTGGGA AACGAAGAAG GGTTATTTGT TGTACATGTC 
ACCAAAGATG AAATTATTAG AGTTGGTGAC AATAAGAAGA TTCATCAGAT TGAACTCATT 
CCAAATGATC AGCTTGTTGC TGTGATCTCA GGACGAAATC GTCATGTACG ACTTTTTCCT 
ATGTCAGCAT TGGATGGGCG AGAGACCGAT TTTTACAAGC TGTCAGAAAC TAAAGGGTGT 
CAAACCGTAA CTTCTGGAAA GGTGCGCCAT GGAGCTCTCA CATGCCTGTG TGTGGCTATG 
AAAAGGCAGG TCCTCTGTTA TGAACTATTT CAGAGCAAGA CCCGTCACAG AAAATTTAAA 
GAAATTCAAG TCCCATATAA TGTCCAGTGG ATGGCAATCT TCAGTGAACA ACTCTGTGTG 
GGATTCCAGT CAGGATTTCT AAGATACCCC TTGAATGGAG AAGGAAATCC ATACAGTATG 
CTCCATTCAA ATGACCATAC ACTATCATTT ATTGCACATC AACCAATGGA TGCTATCTGC 
GCAGTTGAGA TCTCCAGTAA AGAATATCTG CTGTGTTTTA ACAGCATTGG GATATACACT 
GACTGCCAGG GCCGAAGATC TAGACAACAG GAATTGATGT GGCCAGCAAA TCCTTCCTCT 
TGTTGTTACA ATGCACCATA TCTCTCGGTG TACAGTGAAA ATGCAGTTGA TATCTTTGAT 
GTGAACTCCA TGGAATGGAT TCAGACTCTT CCTCTCAAAA AGGTTCGACC CTTAAACAAT 
GAAGGATCAT TAAATCTTTT AGGGTTGGAG ACCATTAGAT TAATATATTT CAAAAATAAG 
ATGGCAGAAG GGGACGAACT GGTAGTACCT GAAACATCAG ATAATAGTCG GAAACAAATG 
GTTAGAAACA TTAACAATAA GCGGCGTTAT TCCTTCAGAG TCCCAGAAGA GGAAAGGATG 
CAGCAGAGGA GGGAAATGCT ACGAGATCCA GAAATGAGAA ATAAATTAAT TTCTAATCCA 
ACTAATTTTA ATCACATAGC ACACATGGGT CCTGGAGATG GAATACAGAT CCTGAAAGAT 
CTGCCCATGA ACCCTCGGCC TCAGGAAAGT CGGACAGTAT TCAGTGGCTC AGTCAGTATT 
CCATCTATCA CCAAATCCCG CCCTGAGCCA GGCCGCTCCA TGAGTGCTAG CAGTGGCTTG 
TCAGCAAGGT CATCCGCACA GAATGGCAGC GCATTAAAGA GGGAATTCTC TGGAGGAAGC 
TACAGTGCCA AGCGGCAGCC CATGCCCTCC CCGTCAGAGG GCTCTTTGTC CTCTGGAGGC 
ATGGACCAAG GAAGTGATGC CCCAGCGAGG GACTTTGACG GAGAGGACTC TGACTCTCCG 
AGGCATTCCA CAGCTTCCAA CAGTTCCAAC CTAAGCAGCC CCCCAAGCCC AGCTTCACCC 
CGAAAAACCA AGAGCCTCTC CCTGGAGAGC ACTGACCGCG GGAGCTGGGA CCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055641.3
CDS	944..5860
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKLEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	NM_014826.4
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014826.4

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGCTG GAAGTTCATA CAGAAGCTCT AGCTGCTGAA 
GCATCTAAAG ACAGGAAGCT ACGTGAACAG AGTGAGCACT ATTCTAAGCA ACTGGAAAAT 
GAATTGGAGG GACTGAAGCA AAAACAAATT AGTTACTCAC CAGGAGTATG CAGCATAGAA 
CATCAGCAAG AGATAACCAA ACTAAAGACT GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAATTAT CTAAAAGAGA AGGAATACAT GCAAATGAAA TAAAAAATCT TAAGAAAGAA 
CTGCATGATT CAGAAGGTCA GCAACTTGCT CTCAACAAAG AAATTATGAT TTTAAAAGAC 
AAATTGGAAA AAACCAGAAG AGAAAGTCAA AGTGAAAGGG AGGAATTTGA AAGTGAGTTC 
AAACAACAAT ATGAACGAGA AAAAGTGTTG TTAACTGAAG AAAATAAAAA GCTGACGAGT 
GAACTTGATA AGCTTACTAC TTTGTATGAG AACTTAAGTA TACACAACCA GCAGTTAGAA 
GAAGAGGTTA AAGATCTAGC AGACAAGAAA GAATCAGTTG CACATTGGGA AGCCCAAATC 
ACAGAAATAA TTCAGTGGGT CAGCGATGAA AAGGATGCAC GAGGGTATCT TCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAGGCA TTAAGAAATT CCAGCTTGGG TACACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCGAAACTGG ATATGTCAGC TAGACTGGAG 
TTGCAGTCGG CTCTGGATGC AGAAATAAGA GCCAAACAGG CCATCCAAGA AGAGTTGAAT 
AAAGTTAAAG CATCTAATAT CATAACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAACTAC TCTCAGAAAT CGAACAGCTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AACTGTAGAC TCCACTCCAC TTTCAGTTCA CACACCAACC 
TTAAGGAAAA AAGGATGTCC TGGTTCAACT GGCTTTCCAC CTAAGCGCAA GACTCACCAG 
TTTTTTGTAA AATCTTTTAC TACTCCTACC AAGTGTCATC AGTGTACCTC CTTGATGGTG 
GGTTTAATAA GACAGGGCTG TTCATGTGAA GTGTGTGGAT TCTCATGCCA TATAACTTGT 
GTAAACAAAG CTCCAACCAC TTGTCCAGTT CCTCCTGAAC AGACAAAAGG TCCCCTGGGT 
ATAGATCCTC AGAAAGGAAT AGGAACAGCA TATGAAGGTC ATGTCAGGAT TCCTAAGCCA 
GCTGGAGTGA AGAAAGGGTG GCAGAGAGCA CTGGCTATAG TGTGTGACTT CAAACTCTTT 
CTGTACGATA TTGCTGAAGG AAAAGCATCT CAGCCCAGTG TTGTCATTAG TCAAGTGATT 
GACATGAGGG ATGAAGAATT TTCTGTGAGT TCAGTCTTGG CTTCTGATGT TATCCATGCA 
AGTCGGAAAG ATATACCCTG TATATTTAGG GTCACAGCTT CCCAGCTCTC AGCATCTAAT 
AACAAATGTT CAATCCTGAT GCTAGCAGAC ACTGAGAATG AGAAGAATAA GTGGGTGGGA 
GTGCTGAGTG AATTGCACAA GATTTTGAAG AAAAACAAAT TCAGAGACCG CTCAGTCTAT 
GTTCCCAAAG AGGCTTATGA CAGCACTCTA CCCCTCATTA AAACAACCCA GGCAGCCGCA 
ATCATAGATC ATGAAAGAAT TGCTTTGGGA AACGAAGAAG GGTTATTTGT TGTACATGTC 
ACCAAAGATG AAATTATTAG AGTTGGTGAC AATAAGAAGA TTCATCAGAT TGAACTCATT 
CCAAATGATC AGCTTGTTGC TGTGATCTCA GGACGAAATC GTCATGTACG ACTTTTTCCT 
ATGTCAGCAT TGGATGGGCG AGAGACCGAT TTTTACAAGC TGTCAGAAAC TAAAGGGTGT 
CAAACCGTAA CTTCTGGAAA GGTGCGCCAT GGAGCTCTCA CATGCCTGTG TGTGGCTATG 
AAAAGGCAGG TCCTCTGTTA TGAACTATTT CAGAGCAAGA CCCGTCACAG AAAATTTAAA 
GAAATTCAAG TCCCATATAA TGTCCAGTGG ATGGCAATCT TCAGTGAACA ACTCTGTGTG 
GGATTCCAGT CAGGATTTCT AAGATACCCC TTGAATGGAG AAGGAAATCC ATACAGTATG 
CTCCATTCAA ATGACCATAC ACTATCATTT ATTGCACATC AACCAATGGA TGCTATCTGC 
GCAGTTGAGA TCTCCAGTAA AGAATATCTG CTGTGTTTTA ACAGCATTGG GATATACACT 
GACTGCCAGG GCCGAAGATC TAGACAACAG GAATTGATGT GGCCAGCAAA TCCTTCCTCT 
TGTTGTTACA ATGCACCATA TCTCTCGGTG TACAGTGAAA ATGCAGTTGA TATCTTTGAT 
GTGAACTCCA TGGAATGGAT TCAGACTCTT CCTCTCAAAA AGGTTCGACC CTTAAACAAT 
GAAGGATCAT TAAATCTTTT AGGGTTGGAG ACCATTAGAT TAATATATTT CAAAAATAAG 
ATGGCAGAAG GGGACGAACT GGTAGTACCT GAAACATCAG ATAATAGTCG GAAACAAATG 
GTTAGAAACA TTAACAATAA GCGGCGTTAT TCCTTCAGAG TCCCAGAAGA GGAAAGGATG 
CAGCAGAGGA GGGAAATGCT ACGAGATCCA GAAATGAGAA ATAAATTAAT TTCTAATCCA 
ACTAATTTTA ATCACATAGC ACACATGGGT CCTGGAGATG GAATACAGAT CCTGAAAGAT 
CTGCCCATGA ACCCTCGGCC TCAGGAAAGT CGGACAGTAT TCAGTGGCTC AGTCAGTATT 
CCATCTATCA CCAAATCCCG CCCTGAGCCA GGCCGCTCCA TGAGTGCTAG CAGTGGCTTG 
TCAGCAAGGT CATCCGCACA GAATGGCAGC GCATTAAAGA GGGAATTCTC TGGAGGAAGC 
TACAGTGCCA AGCGGCAGCC CATGCCCTCC CCGTCAGAGG GCTCTTTGTC CTCTGGAGGC 
ATGGACCAAG GAAGTGATGC CCCAGCGAGG GACTTTGACG GAGAGGACTC TGACTCTCCG 
AGGCATTCCA CAGCTTCCAA CAGTTCCAAC CTAAGCAGCC CCCCAAGCCC AGCTTCACCC 
CGAAAAACCA AGAGCCTCTC CCTGGAGAGC ACTGACCGCG GGAGCTGGGA CCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( NM_014826.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_055641.3 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant A, mRNA.

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CloneID	OHu06683
Clone ID Related Accession (Same CDS sequence)	NM_014826.4 , NM_014826.5
Accession Version	NM_014826.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL627308.20, BQ232716.1, AJ518976.1, BX648219.1, BF765241.1, BC050335.1 and AL353689.26. On Jun 2, 2019 this sequence version replaced NM_014826.4. Transcript Variant: This variant (A) lacks a coding segment compared to variant B, resulting in an isoform (A) that lacks an internal region, as compared to isoform B. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ518976.1, AY166586.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGCTG GAAGTTCATA CAGAAGCTCT AGCTGCTGAA 
GCATCTAAAG ACAGGAAGCT ACGTGAACAG AGTGAGCACT ATTCTAAGCA ACTGGAAAAT 
GAATTGGAGG GACTGAAGCA AAAACAAATT AGTTACTCAC CAGGAGTATG CAGCATAGAA 
CATCAGCAAG AGATAACCAA ACTAAAGACT GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAATTAT CTAAAAGAGA AGGAATACAT GCAAATGAAA TAAAAAATCT TAAGAAAGAA 
CTGCATGATT CAGAAGGTCA GCAACTTGCT CTCAACAAAG AAATTATGAT TTTAAAAGAC 
AAATTGGAAA AAACCAGAAG AGAAAGTCAA AGTGAAAGGG AGGAATTTGA AAGTGAGTTC 
AAACAACAAT ATGAACGAGA AAAAGTGTTG TTAACTGAAG AAAATAAAAA GCTGACGAGT 
GAACTTGATA AGCTTACTAC TTTGTATGAG AACTTAAGTA TACACAACCA GCAGTTAGAA 
GAAGAGGTTA AAGATCTAGC AGACAAGAAA GAATCAGTTG CACATTGGGA AGCCCAAATC 
ACAGAAATAA TTCAGTGGGT CAGCGATGAA AAGGATGCAC GAGGGTATCT TCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAGGCA TTAAGAAATT CCAGCTTGGG TACACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCGAAACTGG ATATGTCAGC TAGACTGGAG 
TTGCAGTCGG CTCTGGATGC AGAAATAAGA GCCAAACAGG CCATCCAAGA AGAGTTGAAT 
AAAGTTAAAG CATCTAATAT CATAACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAACTAC TCTCAGAAAT CGAACAGCTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AACTGTAGAC TCCACTCCAC TTTCAGTTCA CACACCAACC 
TTAAGGAAAA AAGGATGTCC TGGTTCAACT GGCTTTCCAC CTAAGCGCAA GACTCACCAG 
TTTTTTGTAA AATCTTTTAC TACTCCTACC AAGTGTCATC AGTGTACCTC CTTGATGGTG 
GGTTTAATAA GACAGGGCTG TTCATGTGAA GTGTGTGGAT TCTCATGCCA TATAACTTGT 
GTAAACAAAG CTCCAACCAC TTGTCCAGTT CCTCCTGAAC AGACAAAAGG TCCCCTGGGT 
ATAGATCCTC AGAAAGGAAT AGGAACAGCA TATGAAGGTC ATGTCAGGAT TCCTAAGCCA 
GCTGGAGTGA AGAAAGGGTG GCAGAGAGCA CTGGCTATAG TGTGTGACTT CAAACTCTTT 
CTGTACGATA TTGCTGAAGG AAAAGCATCT CAGCCCAGTG TTGTCATTAG TCAAGTGATT 
GACATGAGGG ATGAAGAATT TTCTGTGAGT TCAGTCTTGG CTTCTGATGT TATCCATGCA 
AGTCGGAAAG ATATACCCTG TATATTTAGG GTCACAGCTT CCCAGCTCTC AGCATCTAAT 
AACAAATGTT CAATCCTGAT GCTAGCAGAC ACTGAGAATG AGAAGAATAA GTGGGTGGGA 
GTGCTGAGTG AATTGCACAA GATTTTGAAG AAAAACAAAT TCAGAGACCG CTCAGTCTAT 
GTTCCCAAAG AGGCTTATGA CAGCACTCTA CCCCTCATTA AAACAACCCA GGCAGCCGCA 
ATCATAGATC ATGAAAGAAT TGCTTTGGGA AACGAAGAAG GGTTATTTGT TGTACATGTC 
ACCAAAGATG AAATTATTAG AGTTGGTGAC AATAAGAAGA TTCATCAGAT TGAACTCATT 
CCAAATGATC AGCTTGTTGC TGTGATCTCA GGACGAAATC GTCATGTACG ACTTTTTCCT 
ATGTCAGCAT TGGATGGGCG AGAGACCGAT TTTTACAAGC TGTCAGAAAC TAAAGGGTGT 
CAAACCGTAA CTTCTGGAAA GGTGCGCCAT GGAGCTCTCA CATGCCTGTG TGTGGCTATG 
AAAAGGCAGG TCCTCTGTTA TGAACTATTT CAGAGCAAGA CCCGTCACAG AAAATTTAAA 
GAAATTCAAG TCCCATATAA TGTCCAGTGG ATGGCAATCT TCAGTGAACA ACTCTGTGTG 
GGATTCCAGT CAGGATTTCT AAGATACCCC TTGAATGGAG AAGGAAATCC ATACAGTATG 
CTCCATTCAA ATGACCATAC ACTATCATTT ATTGCACATC AACCAATGGA TGCTATCTGC 
GCAGTTGAGA TCTCCAGTAA AGAATATCTG CTGTGTTTTA ACAGCATTGG GATATACACT 
GACTGCCAGG GCCGAAGATC TAGACAACAG GAATTGATGT GGCCAGCAAA TCCTTCCTCT 
TGTTGTTACA ATGCACCATA TCTCTCGGTG TACAGTGAAA ATGCAGTTGA TATCTTTGAT 
GTGAACTCCA TGGAATGGAT TCAGACTCTT CCTCTCAAAA AGGTTCGACC CTTAAACAAT 
GAAGGATCAT TAAATCTTTT AGGGTTGGAG ACCATTAGAT TAATATATTT CAAAAATAAG 
ATGGCAGAAG GGGACGAACT GGTAGTACCT GAAACATCAG ATAATAGTCG GAAACAAATG 
GTTAGAAACA TTAACAATAA GCGGCGTTAT TCCTTCAGAG TCCCAGAAGA GGAAAGGATG 
CAGCAGAGGA GGGAAATGCT ACGAGATCCA GAAATGAGAA ATAAATTAAT TTCTAATCCA 
ACTAATTTTA ATCACATAGC ACACATGGGT CCTGGAGATG GAATACAGAT CCTGAAAGAT 
CTGCCCATGA ACCCTCGGCC TCAGGAAAGT CGGACAGTAT TCAGTGGCTC AGTCAGTATT 
CCATCTATCA CCAAATCCCG CCCTGAGCCA GGCCGCTCCA TGAGTGCTAG CAGTGGCTTG 
TCAGCAAGGT CATCCGCACA GAATGGCAGC GCATTAAAGA GGGAATTCTC TGGAGGAAGC 
TACAGTGCCA AGCGGCAGCC CATGCCCTCC CCGTCAGAGG GCTCTTTGTC CTCTGGAGGC 
ATGGACCAAG GAAGTGATGC CCCAGCGAGG GACTTTGACG GAGAGGACTC TGACTCTCCG 
AGGCATTCCA CAGCTTCCAA CAGTTCCAAC CTAAGCAGCC CCCCAAGCCC AGCTTCACCC 
CGAAAAACCA AGAGCCTCTC CCTGGAGAGC ACTGACCGCG GGAGCTGGGA CCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055641.3
CDS	1311..6227
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKLEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	NM_014826.5
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014826.5

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGCTG GAAGTTCATA CAGAAGCTCT AGCTGCTGAA 
GCATCTAAAG ACAGGAAGCT ACGTGAACAG AGTGAGCACT ATTCTAAGCA ACTGGAAAAT 
GAATTGGAGG GACTGAAGCA AAAACAAATT AGTTACTCAC CAGGAGTATG CAGCATAGAA 
CATCAGCAAG AGATAACCAA ACTAAAGACT GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAATTAT CTAAAAGAGA AGGAATACAT GCAAATGAAA TAAAAAATCT TAAGAAAGAA 
CTGCATGATT CAGAAGGTCA GCAACTTGCT CTCAACAAAG AAATTATGAT TTTAAAAGAC 
AAATTGGAAA AAACCAGAAG AGAAAGTCAA AGTGAAAGGG AGGAATTTGA AAGTGAGTTC 
AAACAACAAT ATGAACGAGA AAAAGTGTTG TTAACTGAAG AAAATAAAAA GCTGACGAGT 
GAACTTGATA AGCTTACTAC TTTGTATGAG AACTTAAGTA TACACAACCA GCAGTTAGAA 
GAAGAGGTTA AAGATCTAGC AGACAAGAAA GAATCAGTTG CACATTGGGA AGCCCAAATC 
ACAGAAATAA TTCAGTGGGT CAGCGATGAA AAGGATGCAC GAGGGTATCT TCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAGGCA TTAAGAAATT CCAGCTTGGG TACACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCGAAACTGG ATATGTCAGC TAGACTGGAG 
TTGCAGTCGG CTCTGGATGC AGAAATAAGA GCCAAACAGG CCATCCAAGA AGAGTTGAAT 
AAAGTTAAAG CATCTAATAT CATAACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAACTAC TCTCAGAAAT CGAACAGCTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AACTGTAGAC TCCACTCCAC TTTCAGTTCA CACACCAACC 
TTAAGGAAAA AAGGATGTCC TGGTTCAACT GGCTTTCCAC CTAAGCGCAA GACTCACCAG 
TTTTTTGTAA AATCTTTTAC TACTCCTACC AAGTGTCATC AGTGTACCTC CTTGATGGTG 
GGTTTAATAA GACAGGGCTG TTCATGTGAA GTGTGTGGAT TCTCATGCCA TATAACTTGT 
GTAAACAAAG CTCCAACCAC TTGTCCAGTT CCTCCTGAAC AGACAAAAGG TCCCCTGGGT 
ATAGATCCTC AGAAAGGAAT AGGAACAGCA TATGAAGGTC ATGTCAGGAT TCCTAAGCCA 
GCTGGAGTGA AGAAAGGGTG GCAGAGAGCA CTGGCTATAG TGTGTGACTT CAAACTCTTT 
CTGTACGATA TTGCTGAAGG AAAAGCATCT CAGCCCAGTG TTGTCATTAG TCAAGTGATT 
GACATGAGGG ATGAAGAATT TTCTGTGAGT TCAGTCTTGG CTTCTGATGT TATCCATGCA 
AGTCGGAAAG ATATACCCTG TATATTTAGG GTCACAGCTT CCCAGCTCTC AGCATCTAAT 
AACAAATGTT CAATCCTGAT GCTAGCAGAC ACTGAGAATG AGAAGAATAA GTGGGTGGGA 
GTGCTGAGTG AATTGCACAA GATTTTGAAG AAAAACAAAT TCAGAGACCG CTCAGTCTAT 
GTTCCCAAAG AGGCTTATGA CAGCACTCTA CCCCTCATTA AAACAACCCA GGCAGCCGCA 
ATCATAGATC ATGAAAGAAT TGCTTTGGGA AACGAAGAAG GGTTATTTGT TGTACATGTC 
ACCAAAGATG AAATTATTAG AGTTGGTGAC AATAAGAAGA TTCATCAGAT TGAACTCATT 
CCAAATGATC AGCTTGTTGC TGTGATCTCA GGACGAAATC GTCATGTACG ACTTTTTCCT 
ATGTCAGCAT TGGATGGGCG AGAGACCGAT TTTTACAAGC TGTCAGAAAC TAAAGGGTGT 
CAAACCGTAA CTTCTGGAAA GGTGCGCCAT GGAGCTCTCA CATGCCTGTG TGTGGCTATG 
AAAAGGCAGG TCCTCTGTTA TGAACTATTT CAGAGCAAGA CCCGTCACAG AAAATTTAAA 
GAAATTCAAG TCCCATATAA TGTCCAGTGG ATGGCAATCT TCAGTGAACA ACTCTGTGTG 
GGATTCCAGT CAGGATTTCT AAGATACCCC TTGAATGGAG AAGGAAATCC ATACAGTATG 
CTCCATTCAA ATGACCATAC ACTATCATTT ATTGCACATC AACCAATGGA TGCTATCTGC 
GCAGTTGAGA TCTCCAGTAA AGAATATCTG CTGTGTTTTA ACAGCATTGG GATATACACT 
GACTGCCAGG GCCGAAGATC TAGACAACAG GAATTGATGT GGCCAGCAAA TCCTTCCTCT 
TGTTGTTACA ATGCACCATA TCTCTCGGTG TACAGTGAAA ATGCAGTTGA TATCTTTGAT 
GTGAACTCCA TGGAATGGAT TCAGACTCTT CCTCTCAAAA AGGTTCGACC CTTAAACAAT 
GAAGGATCAT TAAATCTTTT AGGGTTGGAG ACCATTAGAT TAATATATTT CAAAAATAAG 
ATGGCAGAAG GGGACGAACT GGTAGTACCT GAAACATCAG ATAATAGTCG GAAACAAATG 
GTTAGAAACA TTAACAATAA GCGGCGTTAT TCCTTCAGAG TCCCAGAAGA GGAAAGGATG 
CAGCAGAGGA GGGAAATGCT ACGAGATCCA GAAATGAGAA ATAAATTAAT TTCTAATCCA 
ACTAATTTTA ATCACATAGC ACACATGGGT CCTGGAGATG GAATACAGAT CCTGAAAGAT 
CTGCCCATGA ACCCTCGGCC TCAGGAAAGT CGGACAGTAT TCAGTGGCTC AGTCAGTATT 
CCATCTATCA CCAAATCCCG CCCTGAGCCA GGCCGCTCCA TGAGTGCTAG CAGTGGCTTG 
TCAGCAAGGT CATCCGCACA GAATGGCAGC GCATTAAAGA GGGAATTCTC TGGAGGAAGC 
TACAGTGCCA AGCGGCAGCC CATGCCCTCC CCGTCAGAGG GCTCTTTGTC CTCTGGAGGC 
ATGGACCAAG GAAGTGATGC CCCAGCGAGG GACTTTGACG GAGAGGACTC TGACTCTCCG 
AGGCATTCCA CAGCTTCCAA CAGTTCCAAC CTAAGCAGCC CCCCAAGCCC AGCTTCACCC 
CGAAAAACCA AGAGCCTCTC CCTGGAGAGC ACTGACCGCG GGAGCTGGGA CCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( NM_001366019.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001352948.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant C, mRNA.

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CloneID	OHu35146
Clone ID Related Accession (Same CDS sequence)	NM_001366019.1 , NM_001366019.2 , XM_005273324.3
Accession Version	NM_001366019.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-27
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL627308.20, AL451047.7 and AL353689.26. On Sep 20, 2018 this sequence version replaced XM_005273324.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGTAG ACTCCACTCC ACTTTCAGTT CACACACCAA CCTTAAGGAA AAAAGGATGT 
CCTGGTTCAA CTGGCTTTCC ACCTAAGCGC AAGACTCACC AGTTTTTTGT AAAATCTTTT 
ACTACTCCTA CCAAGTGTCA TCAGTGTACC TCCTTGATGG TGGGTTTAAT AAGACAGGGC 
TGTTCATGTG AAGTGTGTGG ATTCTCATGC CATATAACTT GTGTAAACAA AGCTCCAACC 
ACTTGTCCAG TTCCTCCTGA ACAGACAAAA GGTCCCCTGG GTATAGATCC TCAGAAAGGA 
ATAGGAACAG CATATGAAGG TCATGTCAGG ATTCCTAAGC CAGCTGGAGT GAAGAAAGGG 
TGGCAGAGAG CACTGGCTAT AGTGTGTGAC TTCAAACTCT TTCTGTACGA TATTGCTGAA 
GGAAAAGCAT CTCAGCCCAG TGTTGTCATT AGTCAAGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT GTTATCCATG CAAGTCGGAA AGATATACCC 
TGTATATTTA GGGTCACAGC TTCCCAGCTC TCAGCATCTA ATAACAAATG TTCAATCCTG 
ATGCTAGCAG ACACTGAGAA TGAGAAGAAT AAGTGGGTGG GAGTGCTGAG TGAATTGCAC 
AAGATTTTGA AGAAAAACAA ATTCAGAGAC CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT 
GACAGCACTC TACCCCTCAT TAAAACAACC CAGGCAGCCG CAATCATAGA TCATGAAAGA 
ATTGCTTTGG GAAACGAAGA AGGGTTATTT GTTGTACATG TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ACAATAAGAA GATTCATCAG ATTGAACTCA TTCCAAATGA TCAGCTTGTT 
GCTGTGATCT CAGGACGAAA TCGTCATGTA CGACTTTTTC CTATGTCAGC ATTGGATGGG 
CGAGAGACCG ATTTTTACAA GCTGTCAGAA ACTAAAGGGT GTCAAACCGT AACTTCTGGA 
AAGGTGCGCC ATGGAGCTCT CACATGCCTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACCCGTCAC AGAAAATTTA AAGAAATTCA AGTCCCATAT 
AATGTCCAGT GGATGGCAAT CTTCAGTGAA CAACTCTGTG TGGGATTCCA GTCAGGATTT 
CTAAGATACC CCTTGAATGG AGAAGGAAAT CCATACAGTA TGCTCCATTC AAATGACCAT 
ACACTATCAT TTATTGCACA TCAACCAATG GATGCTATCT GCGCAGTTGA GATCTCCAGT 
AAAGAATATC TGCTGTGTTT TAACAGCATT GGGATATACA CTGACTGCCA GGGCCGAAGA 
TCTAGACAAC AGGAATTGAT GTGGCCAGCA AATCCTTCCT CTTGTTGTTA CAATGCACCA 
TATCTCTCGG TGTACAGTGA AAATGCAGTT GATATCTTTG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TTCCTCTCAA AAAGGTTCGA CCCTTAAACA ATGAAGGATC ATTAAATCTT 
TTAGGGTTGG AGACCATTAG ATTAATATAT TTCAAAAATA AGATGGCAGA AGGGGACGAA 
CTGGTAGTAC CTGAAACATC AGATAATAGT CGGAAACAAA TGGTTAGAAA CATTAACAAT 
AAGCGGCGTT ATTCCTTCAG AGTCCCAGAA GAGGAAAGGA TGCAGCAGAG GAGGGAAATG 
CTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAATC CAACTAATTT TAATCACATA 
GCACACATGG GTCCTGGAGA TGGAATACAG ATCCTGAAAG ATCTGCCCAT GAACCCTCGG 
CCTCAGGAAA GTCGGACAGT ATTCAGTGGC TCAGTCAGTA TTCCATCTAT CACCAAATCC 
CGCCCTGAGC CAGGCCGCTC CATGAGTGCT AGCAGTGGCT TGTCAGCAAG GTCATCCGCA 
CAGAATGGCA GCGCATTAAA GAGGGAATTC TCTGGAGGAA GCTACAGTGC CAAGCGGCAG 
CCCATGCCCT CCCCGTCAGA GGGCTCTTTG TCCTCTGGAG GCATGGACCA AGGAAGTGAT 
GCCCCAGCGA GGGACTTTGA CGGAGAGGAC TCTGACTCTC CGAGGCATTC CACAGCTTCC 
AACAGTTCCA ACCTAAGCAG CCCCCCAAGC CCAGCTTCAC CCCGAAAAAC CAAGAGCCTC 
TCCCTGGAGA GCACTGACCG CGGGAGCTGG GACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352948.1
CDS	1311..6509
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	NM_001366019.1
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001366019.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGTAG ACTCCACTCC ACTTTCAGTT CACACACCAA CCTTAAGGAA AAAAGGATGT 
CCTGGTTCAA CTGGCTTTCC ACCTAAGCGC AAGACTCACC AGTTTTTTGT AAAATCTTTT 
ACTACTCCTA CCAAGTGTCA TCAGTGTACC TCCTTGATGG TGGGTTTAAT AAGACAGGGC 
TGTTCATGTG AAGTGTGTGG ATTCTCATGC CATATAACTT GTGTAAACAA AGCTCCAACC 
ACTTGTCCAG TTCCTCCTGA ACAGACAAAA GGTCCCCTGG GTATAGATCC TCAGAAAGGA 
ATAGGAACAG CATATGAAGG TCATGTCAGG ATTCCTAAGC CAGCTGGAGT GAAGAAAGGG 
TGGCAGAGAG CACTGGCTAT AGTGTGTGAC TTCAAACTCT TTCTGTACGA TATTGCTGAA 
GGAAAAGCAT CTCAGCCCAG TGTTGTCATT AGTCAAGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT GTTATCCATG CAAGTCGGAA AGATATACCC 
TGTATATTTA GGGTCACAGC TTCCCAGCTC TCAGCATCTA ATAACAAATG TTCAATCCTG 
ATGCTAGCAG ACACTGAGAA TGAGAAGAAT AAGTGGGTGG GAGTGCTGAG TGAATTGCAC 
AAGATTTTGA AGAAAAACAA ATTCAGAGAC CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT 
GACAGCACTC TACCCCTCAT TAAAACAACC CAGGCAGCCG CAATCATAGA TCATGAAAGA 
ATTGCTTTGG GAAACGAAGA AGGGTTATTT GTTGTACATG TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ACAATAAGAA GATTCATCAG ATTGAACTCA TTCCAAATGA TCAGCTTGTT 
GCTGTGATCT CAGGACGAAA TCGTCATGTA CGACTTTTTC CTATGTCAGC ATTGGATGGG 
CGAGAGACCG ATTTTTACAA GCTGTCAGAA ACTAAAGGGT GTCAAACCGT AACTTCTGGA 
AAGGTGCGCC ATGGAGCTCT CACATGCCTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACCCGTCAC AGAAAATTTA AAGAAATTCA AGTCCCATAT 
AATGTCCAGT GGATGGCAAT CTTCAGTGAA CAACTCTGTG TGGGATTCCA GTCAGGATTT 
CTAAGATACC CCTTGAATGG AGAAGGAAAT CCATACAGTA TGCTCCATTC AAATGACCAT 
ACACTATCAT TTATTGCACA TCAACCAATG GATGCTATCT GCGCAGTTGA GATCTCCAGT 
AAAGAATATC TGCTGTGTTT TAACAGCATT GGGATATACA CTGACTGCCA GGGCCGAAGA 
TCTAGACAAC AGGAATTGAT GTGGCCAGCA AATCCTTCCT CTTGTTGTTA CAATGCACCA 
TATCTCTCGG TGTACAGTGA AAATGCAGTT GATATCTTTG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TTCCTCTCAA AAAGGTTCGA CCCTTAAACA ATGAAGGATC ATTAAATCTT 
TTAGGGTTGG AGACCATTAG ATTAATATAT TTCAAAAATA AGATGGCAGA AGGGGACGAA 
CTGGTAGTAC CTGAAACATC AGATAATAGT CGGAAACAAA TGGTTAGAAA CATTAACAAT 
AAGCGGCGTT ATTCCTTCAG AGTCCCAGAA GAGGAAAGGA TGCAGCAGAG GAGGGAAATG 
CTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAATC CAACTAATTT TAATCACATA 
GCACACATGG GTCCTGGAGA TGGAATACAG ATCCTGAAAG ATCTGCCCAT GAACCCTCGG 
CCTCAGGAAA GTCGGACAGT ATTCAGTGGC TCAGTCAGTA TTCCATCTAT CACCAAATCC 
CGCCCTGAGC CAGGCCGCTC CATGAGTGCT AGCAGTGGCT TGTCAGCAAG GTCATCCGCA 
CAGAATGGCA GCGCATTAAA GAGGGAATTC TCTGGAGGAA GCTACAGTGC CAAGCGGCAG 
CCCATGCCCT CCCCGTCAGA GGGCTCTTTG TCCTCTGGAG GCATGGACCA AGGAAGTGAT 
GCCCCAGCGA GGGACTTTGA CGGAGAGGAC TCTGACTCTC CGAGGCATTC CACAGCTTCC 
AACAGTTCCA ACCTAAGCAG CCCCCCAAGC CCAGCTTCAC CCCGAAAAAC CAAGAGCCTC 
TCCCTGGAGA GCACTGACCG CGGGAGCTGG GACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( NM_001366019.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001352948.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant C, mRNA.

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CloneID	OHu35146
Clone ID Related Accession (Same CDS sequence)	NM_001366019.1 , NM_001366019.2 , XM_005273324.3
Accession Version	NM_001366019.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL627308.20, AL451047.7 and AL353689.26. On Jun 2, 2019 this sequence version replaced NM_001366019.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGTAG ACTCCACTCC ACTTTCAGTT CACACACCAA CCTTAAGGAA AAAAGGATGT 
CCTGGTTCAA CTGGCTTTCC ACCTAAGCGC AAGACTCACC AGTTTTTTGT AAAATCTTTT 
ACTACTCCTA CCAAGTGTCA TCAGTGTACC TCCTTGATGG TGGGTTTAAT AAGACAGGGC 
TGTTCATGTG AAGTGTGTGG ATTCTCATGC CATATAACTT GTGTAAACAA AGCTCCAACC 
ACTTGTCCAG TTCCTCCTGA ACAGACAAAA GGTCCCCTGG GTATAGATCC TCAGAAAGGA 
ATAGGAACAG CATATGAAGG TCATGTCAGG ATTCCTAAGC CAGCTGGAGT GAAGAAAGGG 
TGGCAGAGAG CACTGGCTAT AGTGTGTGAC TTCAAACTCT TTCTGTACGA TATTGCTGAA 
GGAAAAGCAT CTCAGCCCAG TGTTGTCATT AGTCAAGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT GTTATCCATG CAAGTCGGAA AGATATACCC 
TGTATATTTA GGGTCACAGC TTCCCAGCTC TCAGCATCTA ATAACAAATG TTCAATCCTG 
ATGCTAGCAG ACACTGAGAA TGAGAAGAAT AAGTGGGTGG GAGTGCTGAG TGAATTGCAC 
AAGATTTTGA AGAAAAACAA ATTCAGAGAC CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT 
GACAGCACTC TACCCCTCAT TAAAACAACC CAGGCAGCCG CAATCATAGA TCATGAAAGA 
ATTGCTTTGG GAAACGAAGA AGGGTTATTT GTTGTACATG TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ACAATAAGAA GATTCATCAG ATTGAACTCA TTCCAAATGA TCAGCTTGTT 
GCTGTGATCT CAGGACGAAA TCGTCATGTA CGACTTTTTC CTATGTCAGC ATTGGATGGG 
CGAGAGACCG ATTTTTACAA GCTGTCAGAA ACTAAAGGGT GTCAAACCGT AACTTCTGGA 
AAGGTGCGCC ATGGAGCTCT CACATGCCTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACCCGTCAC AGAAAATTTA AAGAAATTCA AGTCCCATAT 
AATGTCCAGT GGATGGCAAT CTTCAGTGAA CAACTCTGTG TGGGATTCCA GTCAGGATTT 
CTAAGATACC CCTTGAATGG AGAAGGAAAT CCATACAGTA TGCTCCATTC AAATGACCAT 
ACACTATCAT TTATTGCACA TCAACCAATG GATGCTATCT GCGCAGTTGA GATCTCCAGT 
AAAGAATATC TGCTGTGTTT TAACAGCATT GGGATATACA CTGACTGCCA GGGCCGAAGA 
TCTAGACAAC AGGAATTGAT GTGGCCAGCA AATCCTTCCT CTTGTTGTTA CAATGCACCA 
TATCTCTCGG TGTACAGTGA AAATGCAGTT GATATCTTTG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TTCCTCTCAA AAAGGTTCGA CCCTTAAACA ATGAAGGATC ATTAAATCTT 
TTAGGGTTGG AGACCATTAG ATTAATATAT TTCAAAAATA AGATGGCAGA AGGGGACGAA 
CTGGTAGTAC CTGAAACATC AGATAATAGT CGGAAACAAA TGGTTAGAAA CATTAACAAT 
AAGCGGCGTT ATTCCTTCAG AGTCCCAGAA GAGGAAAGGA TGCAGCAGAG GAGGGAAATG 
CTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAATC CAACTAATTT TAATCACATA 
GCACACATGG GTCCTGGAGA TGGAATACAG ATCCTGAAAG ATCTGCCCAT GAACCCTCGG 
CCTCAGGAAA GTCGGACAGT ATTCAGTGGC TCAGTCAGTA TTCCATCTAT CACCAAATCC 
CGCCCTGAGC CAGGCCGCTC CATGAGTGCT AGCAGTGGCT TGTCAGCAAG GTCATCCGCA 
CAGAATGGCA GCGCATTAAA GAGGGAATTC TCTGGAGGAA GCTACAGTGC CAAGCGGCAG 
CCCATGCCCT CCCCGTCAGA GGGCTCTTTG TCCTCTGGAG GCATGGACCA AGGAAGTGAT 
GCCCCAGCGA GGGACTTTGA CGGAGAGGAC TCTGACTCTC CGAGGCATTC CACAGCTTCC 
AACAGTTCCA ACCTAAGCAG CCCCCCAAGC CCAGCTTCAC CCCGAAAAAC CAAGAGCCTC 
TCCCTGGAGA GCACTGACCG CGGGAGCTGG GACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352948.1
CDS	1311..6509
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	NM_001366019.2
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001366019.2

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGTAG ACTCCACTCC ACTTTCAGTT CACACACCAA CCTTAAGGAA AAAAGGATGT 
CCTGGTTCAA CTGGCTTTCC ACCTAAGCGC AAGACTCACC AGTTTTTTGT AAAATCTTTT 
ACTACTCCTA CCAAGTGTCA TCAGTGTACC TCCTTGATGG TGGGTTTAAT AAGACAGGGC 
TGTTCATGTG AAGTGTGTGG ATTCTCATGC CATATAACTT GTGTAAACAA AGCTCCAACC 
ACTTGTCCAG TTCCTCCTGA ACAGACAAAA GGTCCCCTGG GTATAGATCC TCAGAAAGGA 
ATAGGAACAG CATATGAAGG TCATGTCAGG ATTCCTAAGC CAGCTGGAGT GAAGAAAGGG 
TGGCAGAGAG CACTGGCTAT AGTGTGTGAC TTCAAACTCT TTCTGTACGA TATTGCTGAA 
GGAAAAGCAT CTCAGCCCAG TGTTGTCATT AGTCAAGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT GTTATCCATG CAAGTCGGAA AGATATACCC 
TGTATATTTA GGGTCACAGC TTCCCAGCTC TCAGCATCTA ATAACAAATG TTCAATCCTG 
ATGCTAGCAG ACACTGAGAA TGAGAAGAAT AAGTGGGTGG GAGTGCTGAG TGAATTGCAC 
AAGATTTTGA AGAAAAACAA ATTCAGAGAC CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT 
GACAGCACTC TACCCCTCAT TAAAACAACC CAGGCAGCCG CAATCATAGA TCATGAAAGA 
ATTGCTTTGG GAAACGAAGA AGGGTTATTT GTTGTACATG TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ACAATAAGAA GATTCATCAG ATTGAACTCA TTCCAAATGA TCAGCTTGTT 
GCTGTGATCT CAGGACGAAA TCGTCATGTA CGACTTTTTC CTATGTCAGC ATTGGATGGG 
CGAGAGACCG ATTTTTACAA GCTGTCAGAA ACTAAAGGGT GTCAAACCGT AACTTCTGGA 
AAGGTGCGCC ATGGAGCTCT CACATGCCTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACCCGTCAC AGAAAATTTA AAGAAATTCA AGTCCCATAT 
AATGTCCAGT GGATGGCAAT CTTCAGTGAA CAACTCTGTG TGGGATTCCA GTCAGGATTT 
CTAAGATACC CCTTGAATGG AGAAGGAAAT CCATACAGTA TGCTCCATTC AAATGACCAT 
ACACTATCAT TTATTGCACA TCAACCAATG GATGCTATCT GCGCAGTTGA GATCTCCAGT 
AAAGAATATC TGCTGTGTTT TAACAGCATT GGGATATACA CTGACTGCCA GGGCCGAAGA 
TCTAGACAAC AGGAATTGAT GTGGCCAGCA AATCCTTCCT CTTGTTGTTA CAATGCACCA 
TATCTCTCGG TGTACAGTGA AAATGCAGTT GATATCTTTG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TTCCTCTCAA AAAGGTTCGA CCCTTAAACA ATGAAGGATC ATTAAATCTT 
TTAGGGTTGG AGACCATTAG ATTAATATAT TTCAAAAATA AGATGGCAGA AGGGGACGAA 
CTGGTAGTAC CTGAAACATC AGATAATAGT CGGAAACAAA TGGTTAGAAA CATTAACAAT 
AAGCGGCGTT ATTCCTTCAG AGTCCCAGAA GAGGAAAGGA TGCAGCAGAG GAGGGAAATG 
CTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAATC CAACTAATTT TAATCACATA 
GCACACATGG GTCCTGGAGA TGGAATACAG ATCCTGAAAG ATCTGCCCAT GAACCCTCGG 
CCTCAGGAAA GTCGGACAGT ATTCAGTGGC TCAGTCAGTA TTCCATCTAT CACCAAATCC 
CGCCCTGAGC CAGGCCGCTC CATGAGTGCT AGCAGTGGCT TGTCAGCAAG GTCATCCGCA 
CAGAATGGCA GCGCATTAAA GAGGGAATTC TCTGGAGGAA GCTACAGTGC CAAGCGGCAG 
CCCATGCCCT CCCCGTCAGA GGGCTCTTTG TCCTCTGGAG GCATGGACCA AGGAAGTGAT 
GCCCCAGCGA GGGACTTTGA CGGAGAGGAC TCTGACTCTC CGAGGCATTC CACAGCTTCC 
AACAGTTCCA ACCTAAGCAG CCCCCCAAGC CCAGCTTCAC CCCGAAAAAC CAAGAGCCTC 
TCCCTGGAGA GCACTGACCG CGGGAGCTGG GACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_005273317.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005273374.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X2, mRNA.

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CloneID	OHu35137
Clone ID Related Accession (Same CDS sequence)	XM_005273317.3 , XM_005273317.4
Accession Version	XM_005273317.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005273317.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACACTGATCC CGTTGAGAAC ACATATGTAT GGAACCCGAG CGTCAAGTTT 
CACATCCAGT CACGGTCCAC ATCTCCTTCC ACATCTAGTG AAGCTGAGCC AGTTAAGACT 
GTAGACTCCA CTCCACTTTC AGTTCACACA CCAACCTTAA GGAAAAAAGG ATGTCCTGGT 
TCAACTGGCT TTCCACCTAA GCGCAAGACT CACCAGTTTT TTGTAAAATC TTTTACTACT 
CCTACCAAGT GTCATCAGTG TACCTCCTTG ATGGTGGGTT TAATAAGACA GGGCTGTTCA 
TGTGAAGTGT GTGGATTCTC ATGCCATATA ACTTGTGTAA ACAAAGCTCC AACCACTTGT 
CCAGTTCCTC CTGAACAGAC AAAAGGTCCC CTGGGTATAG ATCCTCAGAA AGGAATAGGA 
ACAGCATATG AAGGTCATGT CAGGATTCCT AAGCCAGCTG GAGTGAAGAA AGGGTGGCAG 
AGAGCACTGG CTATAGTGTG TGACTTCAAA CTCTTTCTGT ACGATATTGC TGAAGGAAAA 
GCATCTCAGC CCAGTGTTGT CATTAGTCAA GTGATTGACA TGAGGGATGA AGAATTTTCT 
GTGAGTTCAG TCTTGGCTTC TGATGTTATC CATGCAAGTC GGAAAGATAT ACCCTGTATA 
TTTAGGGTCA CAGCTTCCCA GCTCTCAGCA TCTAATAACA AATGTTCAAT CCTGATGCTA 
GCAGACACTG AGAATGAGAA GAATAAGTGG GTGGGAGTGC TGAGTGAATT GCACAAGATT 
TTGAAGAAAA ACAAATTCAG AGACCGCTCA GTCTATGTTC CCAAAGAGGC TTATGACAGC 
ACTCTACCCC TCATTAAAAC AACCCAGGCA GCCGCAATCA TAGATCATGA AAGAATTGCT 
TTGGGAAACG AAGAAGGGTT ATTTGTTGTA CATGTCACCA AAGATGAAAT TATTAGAGTT 
GGTGACAATA AGAAGATTCA TCAGATTGAA CTCATTCCAA ATGATCAGCT TGTTGCTGTG 
ATCTCAGGAC GAAATCGTCA TGTACGACTT TTTCCTATGT CAGCATTGGA TGGGCGAGAG 
ACCGATTTTT ACAAGCTGTC AGAAACTAAA GGGTGTCAAA CCGTAACTTC TGGAAAGGTG 
CGCCATGGAG CTCTCACATG CCTGTGTGTG GCTATGAAAA GGCAGGTCCT CTGTTATGAA 
CTATTTCAGA GCAAGACCCG TCACAGAAAA TTTAAAGAAA TTCAAGTCCC ATATAATGTC 
CAGTGGATGG CAATCTTCAG TGAACAACTC TGTGTGGGAT TCCAGTCAGG ATTTCTAAGA 
TACCCCTTGA ATGGAGAAGG AAATCCATAC AGTATGCTCC ATTCAAATGA CCATACACTA 
TCATTTATTG CACATCAACC AATGGATGCT ATCTGCGCAG TTGAGATCTC CAGTAAAGAA 
TATCTGCTGT GTTTTAACAG CATTGGGATA TACACTGACT GCCAGGGCCG AAGATCTAGA 
CAACAGGAAT TGATGTGGCC AGCAAATCCT TCCTCTTGTT GTTACAATGC ACCATATCTC 
TCGGTGTACA GTGAAAATGC AGTTGATATC TTTGATGTGA ACTCCATGGA ATGGATTCAG 
ACTCTTCCTC TCAAAAAGGT TCGACCCTTA AACAATGAAG GATCATTAAA TCTTTTAGGG 
TTGGAGACCA TTAGATTAAT ATATTTCAAA AATAAGATGG CAGAAGGGGA CGAACTGGTA 
GTACCTGAAA CATCAGATAA TAGTCGGAAA CAAATGGTTA GAAACATTAA CAATAAGCGG 
CGTTATTCCT TCAGAGTCCC AGAAGAGGAA AGGATGCAGC AGAGGAGGGA AATGCTACGA 
GATCCAGAAA TGAGAAATAA ATTAATTTCT AATCCAACTA ATTTTAATCA CATAGCACAC 
ATGGGTCCTG GAGATGGAAT ACAGATCCTG AAAGATCTGC CCATGCCAGG TTTCCCTTAT 
CCATCCCCTC ATCATCACTC CGGTCTGATC TCCTCTCCGA TCAACTTTGA ACACATCTAT 
CACATGACTG TTAATTCTGC TGAAAAATTT CTCTCTCCTG ATTCCATAAA CCCTGAATAT 
TCCCCGTCTC TGCGCTCTGT CCCTGGTACA CCAAGCTTTA TGACTCTGAG GAACCCTCGG 
CCTCAGGAAA GTCGGACAGT ATTCAGTGGC TCAGTCAGTA TTCCATCTAT CACCAAATCC 
CGCCCTGAGC CAGGCCGCTC CATGAGTGCT AGCAGTGGCT TGTCAGCAAG GTCATCCGCA 
CAGAATGGCA GCGCATTAAA GAGGGAATTC TCTGGAGGAA GCTACAGTGC CAAGCGGCAG 
CCCATGCCCT CCCCGTCAGA GGGCTCTTTG TCCTCTGGAG GCATGGACCA AGGAAGTGAT 
GCCCCAGCGA GGGACTTTGA CGGAGAGGAC TCTGACTCTC CGAGGCATTC CACAGCTTCC 
AACAGTTCCA ACCTAAGCAG CCCCCCAAGC CCAGCTTCAC CCCGAAAAAC CAAGAGCCTC 
TCCCTGGAGA GCACTGACCG CGGGAGCTGG GACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005273374.1
CDS	1056..6554
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDTDPVENTYVWNPSVKFHIQSRSTSPSTSSEAEPVKTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_005273317.3
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005273317.3

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACACTGATCC CGTTGAGAAC ACATATGTAT GGAACCCGAG CGTCAAGTTT 
CACATCCAGT CACGGTCCAC ATCTCCTTCC ACATCTAGTG AAGCTGAGCC AGTTAAGACT 
GTAGACTCCA CTCCACTTTC AGTTCACACA CCAACCTTAA GGAAAAAAGG ATGTCCTGGT 
TCAACTGGCT TTCCACCTAA GCGCAAGACT CACCAGTTTT TTGTAAAATC TTTTACTACT 
CCTACCAAGT GTCATCAGTG TACCTCCTTG ATGGTGGGTT TAATAAGACA GGGCTGTTCA 
TGTGAAGTGT GTGGATTCTC ATGCCATATA ACTTGTGTAA ACAAAGCTCC AACCACTTGT 
CCAGTTCCTC CTGAACAGAC AAAAGGTCCC CTGGGTATAG ATCCTCAGAA AGGAATAGGA 
ACAGCATATG AAGGTCATGT CAGGATTCCT AAGCCAGCTG GAGTGAAGAA AGGGTGGCAG 
AGAGCACTGG CTATAGTGTG TGACTTCAAA CTCTTTCTGT ACGATATTGC TGAAGGAAAA 
GCATCTCAGC CCAGTGTTGT CATTAGTCAA GTGATTGACA TGAGGGATGA AGAATTTTCT 
GTGAGTTCAG TCTTGGCTTC TGATGTTATC CATGCAAGTC GGAAAGATAT ACCCTGTATA 
TTTAGGGTCA CAGCTTCCCA GCTCTCAGCA TCTAATAACA AATGTTCAAT CCTGATGCTA 
GCAGACACTG AGAATGAGAA GAATAAGTGG GTGGGAGTGC TGAGTGAATT GCACAAGATT 
TTGAAGAAAA ACAAATTCAG AGACCGCTCA GTCTATGTTC CCAAAGAGGC TTATGACAGC 
ACTCTACCCC TCATTAAAAC AACCCAGGCA GCCGCAATCA TAGATCATGA AAGAATTGCT 
TTGGGAAACG AAGAAGGGTT ATTTGTTGTA CATGTCACCA AAGATGAAAT TATTAGAGTT 
GGTGACAATA AGAAGATTCA TCAGATTGAA CTCATTCCAA ATGATCAGCT TGTTGCTGTG 
ATCTCAGGAC GAAATCGTCA TGTACGACTT TTTCCTATGT CAGCATTGGA TGGGCGAGAG 
ACCGATTTTT ACAAGCTGTC AGAAACTAAA GGGTGTCAAA CCGTAACTTC TGGAAAGGTG 
CGCCATGGAG CTCTCACATG CCTGTGTGTG GCTATGAAAA GGCAGGTCCT CTGTTATGAA 
CTATTTCAGA GCAAGACCCG TCACAGAAAA TTTAAAGAAA TTCAAGTCCC ATATAATGTC 
CAGTGGATGG CAATCTTCAG TGAACAACTC TGTGTGGGAT TCCAGTCAGG ATTTCTAAGA 
TACCCCTTGA ATGGAGAAGG AAATCCATAC AGTATGCTCC ATTCAAATGA CCATACACTA 
TCATTTATTG CACATCAACC AATGGATGCT ATCTGCGCAG TTGAGATCTC CAGTAAAGAA 
TATCTGCTGT GTTTTAACAG CATTGGGATA TACACTGACT GCCAGGGCCG AAGATCTAGA 
CAACAGGAAT TGATGTGGCC AGCAAATCCT TCCTCTTGTT GTTACAATGC ACCATATCTC 
TCGGTGTACA GTGAAAATGC AGTTGATATC TTTGATGTGA ACTCCATGGA ATGGATTCAG 
ACTCTTCCTC TCAAAAAGGT TCGACCCTTA AACAATGAAG GATCATTAAA TCTTTTAGGG 
TTGGAGACCA TTAGATTAAT ATATTTCAAA AATAAGATGG CAGAAGGGGA CGAACTGGTA 
GTACCTGAAA CATCAGATAA TAGTCGGAAA CAAATGGTTA GAAACATTAA CAATAAGCGG 
CGTTATTCCT TCAGAGTCCC AGAAGAGGAA AGGATGCAGC AGAGGAGGGA AATGCTACGA 
GATCCAGAAA TGAGAAATAA ATTAATTTCT AATCCAACTA ATTTTAATCA CATAGCACAC 
ATGGGTCCTG GAGATGGAAT ACAGATCCTG AAAGATCTGC CCATGCCAGG TTTCCCTTAT 
CCATCCCCTC ATCATCACTC CGGTCTGATC TCCTCTCCGA TCAACTTTGA ACACATCTAT 
CACATGACTG TTAATTCTGC TGAAAAATTT CTCTCTCCTG ATTCCATAAA CCCTGAATAT 
TCCCCGTCTC TGCGCTCTGT CCCTGGTACA CCAAGCTTTA TGACTCTGAG GAACCCTCGG 
CCTCAGGAAA GTCGGACAGT ATTCAGTGGC TCAGTCAGTA TTCCATCTAT CACCAAATCC 
CGCCCTGAGC CAGGCCGCTC CATGAGTGCT AGCAGTGGCT TGTCAGCAAG GTCATCCGCA 
CAGAATGGCA GCGCATTAAA GAGGGAATTC TCTGGAGGAA GCTACAGTGC CAAGCGGCAG 
CCCATGCCCT CCCCGTCAGA GGGCTCTTTG TCCTCTGGAG GCATGGACCA AGGAAGTGAT 
GCCCCAGCGA GGGACTTTGA CGGAGAGGAC TCTGACTCTC CGAGGCATTC CACAGCTTCC 
AACAGTTCCA ACCTAAGCAG CCCCCCAAGC CCAGCTTCAC CCCGAAAAAC CAAGAGCCTC 
TCCCTGGAGA GCACTGACCG CGGGAGCTGG GACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_005273317.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005273374.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X2, mRNA.

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CloneID	OHu35137
Clone ID Related Accession (Same CDS sequence)	XM_005273317.3 , XM_005273317.4
Accession Version	XM_005273317.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005273317.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACACTGATCC CGTTGAGAAC ACATATGTAT GGAACCCGAG CGTCAAGTTT 
CACATCCAGT CACGGTCCAC ATCTCCTTCC ACATCTAGTG AAGCTGAGCC AGTTAAGACT 
GTAGACTCCA CTCCACTTTC AGTTCACACA CCAACCTTAA GGAAAAAAGG ATGTCCTGGT 
TCAACTGGCT TTCCACCTAA GCGCAAGACT CACCAGTTTT TTGTAAAATC TTTTACTACT 
CCTACCAAGT GTCATCAGTG TACCTCCTTG ATGGTGGGTT TAATAAGACA GGGCTGTTCA 
TGTGAAGTGT GTGGATTCTC ATGCCATATA ACTTGTGTAA ACAAAGCTCC AACCACTTGT 
CCAGTTCCTC CTGAACAGAC AAAAGGTCCC CTGGGTATAG ATCCTCAGAA AGGAATAGGA 
ACAGCATATG AAGGTCATGT CAGGATTCCT AAGCCAGCTG GAGTGAAGAA AGGGTGGCAG 
AGAGCACTGG CTATAGTGTG TGACTTCAAA CTCTTTCTGT ACGATATTGC TGAAGGAAAA 
GCATCTCAGC CCAGTGTTGT CATTAGTCAA GTGATTGACA TGAGGGATGA AGAATTTTCT 
GTGAGTTCAG TCTTGGCTTC TGATGTTATC CATGCAAGTC GGAAAGATAT ACCCTGTATA 
TTTAGGGTCA CAGCTTCCCA GCTCTCAGCA TCTAATAACA AATGTTCAAT CCTGATGCTA 
GCAGACACTG AGAATGAGAA GAATAAGTGG GTGGGAGTGC TGAGTGAATT GCACAAGATT 
TTGAAGAAAA ACAAATTCAG AGACCGCTCA GTCTATGTTC CCAAAGAGGC TTATGACAGC 
ACTCTACCCC TCATTAAAAC AACCCAGGCA GCCGCAATCA TAGATCATGA AAGAATTGCT 
TTGGGAAACG AAGAAGGGTT ATTTGTTGTA CATGTCACCA AAGATGAAAT TATTAGAGTT 
GGTGACAATA AGAAGATTCA TCAGATTGAA CTCATTCCAA ATGATCAGCT TGTTGCTGTG 
ATCTCAGGAC GAAATCGTCA TGTACGACTT TTTCCTATGT CAGCATTGGA TGGGCGAGAG 
ACCGATTTTT ACAAGCTGTC AGAAACTAAA GGGTGTCAAA CCGTAACTTC TGGAAAGGTG 
CGCCATGGAG CTCTCACATG CCTGTGTGTG GCTATGAAAA GGCAGGTCCT CTGTTATGAA 
CTATTTCAGA GCAAGACCCG TCACAGAAAA TTTAAAGAAA TTCAAGTCCC ATATAATGTC 
CAGTGGATGG CAATCTTCAG TGAACAACTC TGTGTGGGAT TCCAGTCAGG ATTTCTAAGA 
TACCCCTTGA ATGGAGAAGG AAATCCATAC AGTATGCTCC ATTCAAATGA CCATACACTA 
TCATTTATTG CACATCAACC AATGGATGCT ATCTGCGCAG TTGAGATCTC CAGTAAAGAA 
TATCTGCTGT GTTTTAACAG CATTGGGATA TACACTGACT GCCAGGGCCG AAGATCTAGA 
CAACAGGAAT TGATGTGGCC AGCAAATCCT TCCTCTTGTT GTTACAATGC ACCATATCTC 
TCGGTGTACA GTGAAAATGC AGTTGATATC TTTGATGTGA ACTCCATGGA ATGGATTCAG 
ACTCTTCCTC TCAAAAAGGT TCGACCCTTA AACAATGAAG GATCATTAAA TCTTTTAGGG 
TTGGAGACCA TTAGATTAAT ATATTTCAAA AATAAGATGG CAGAAGGGGA CGAACTGGTA 
GTACCTGAAA CATCAGATAA TAGTCGGAAA CAAATGGTTA GAAACATTAA CAATAAGCGG 
CGTTATTCCT TCAGAGTCCC AGAAGAGGAA AGGATGCAGC AGAGGAGGGA AATGCTACGA 
GATCCAGAAA TGAGAAATAA ATTAATTTCT AATCCAACTA ATTTTAATCA CATAGCACAC 
ATGGGTCCTG GAGATGGAAT ACAGATCCTG AAAGATCTGC CCATGCCAGG TTTCCCTTAT 
CCATCCCCTC ATCATCACTC CGGTCTGATC TCCTCTCCGA TCAACTTTGA ACACATCTAT 
CACATGACTG TTAATTCTGC TGAAAAATTT CTCTCTCCTG ATTCCATAAA CCCTGAATAT 
TCCCCGTCTC TGCGCTCTGT CCCTGGTACA CCAAGCTTTA TGACTCTGAG GAACCCTCGG 
CCTCAGGAAA GTCGGACAGT ATTCAGTGGC TCAGTCAGTA TTCCATCTAT CACCAAATCC 
CGCCCTGAGC CAGGCCGCTC CATGAGTGCT AGCAGTGGCT TGTCAGCAAG GTCATCCGCA 
CAGAATGGCA GCGCATTAAA GAGGGAATTC TCTGGAGGAA GCTACAGTGC CAAGCGGCAG 
CCCATGCCCT CCCCGTCAGA GGGCTCTTTG TCCTCTGGAG GCATGGACCA AGGAAGTGAT 
GCCCCAGCGA GGGACTTTGA CGGAGAGGAC TCTGACTCTC CGAGGCATTC CACAGCTTCC 
AACAGTTCCA ACCTAAGCAG CCCCCCAAGC CCAGCTTCAC CCCGAAAAAC CAAGAGCCTC 
TCCCTGGAGA GCACTGACCG CGGGAGCTGG GACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005273374.1
CDS	969..6467
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDTDPVENTYVWNPSVKFHIQSRSTSPSTSSEAEPVKTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_005273317.4
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005273317.4

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACACTGATCC CGTTGAGAAC ACATATGTAT GGAACCCGAG CGTCAAGTTT 
CACATCCAGT CACGGTCCAC ATCTCCTTCC ACATCTAGTG AAGCTGAGCC AGTTAAGACT 
GTAGACTCCA CTCCACTTTC AGTTCACACA CCAACCTTAA GGAAAAAAGG ATGTCCTGGT 
TCAACTGGCT TTCCACCTAA GCGCAAGACT CACCAGTTTT TTGTAAAATC TTTTACTACT 
CCTACCAAGT GTCATCAGTG TACCTCCTTG ATGGTGGGTT TAATAAGACA GGGCTGTTCA 
TGTGAAGTGT GTGGATTCTC ATGCCATATA ACTTGTGTAA ACAAAGCTCC AACCACTTGT 
CCAGTTCCTC CTGAACAGAC AAAAGGTCCC CTGGGTATAG ATCCTCAGAA AGGAATAGGA 
ACAGCATATG AAGGTCATGT CAGGATTCCT AAGCCAGCTG GAGTGAAGAA AGGGTGGCAG 
AGAGCACTGG CTATAGTGTG TGACTTCAAA CTCTTTCTGT ACGATATTGC TGAAGGAAAA 
GCATCTCAGC CCAGTGTTGT CATTAGTCAA GTGATTGACA TGAGGGATGA AGAATTTTCT 
GTGAGTTCAG TCTTGGCTTC TGATGTTATC CATGCAAGTC GGAAAGATAT ACCCTGTATA 
TTTAGGGTCA CAGCTTCCCA GCTCTCAGCA TCTAATAACA AATGTTCAAT CCTGATGCTA 
GCAGACACTG AGAATGAGAA GAATAAGTGG GTGGGAGTGC TGAGTGAATT GCACAAGATT 
TTGAAGAAAA ACAAATTCAG AGACCGCTCA GTCTATGTTC CCAAAGAGGC TTATGACAGC 
ACTCTACCCC TCATTAAAAC AACCCAGGCA GCCGCAATCA TAGATCATGA AAGAATTGCT 
TTGGGAAACG AAGAAGGGTT ATTTGTTGTA CATGTCACCA AAGATGAAAT TATTAGAGTT 
GGTGACAATA AGAAGATTCA TCAGATTGAA CTCATTCCAA ATGATCAGCT TGTTGCTGTG 
ATCTCAGGAC GAAATCGTCA TGTACGACTT TTTCCTATGT CAGCATTGGA TGGGCGAGAG 
ACCGATTTTT ACAAGCTGTC AGAAACTAAA GGGTGTCAAA CCGTAACTTC TGGAAAGGTG 
CGCCATGGAG CTCTCACATG CCTGTGTGTG GCTATGAAAA GGCAGGTCCT CTGTTATGAA 
CTATTTCAGA GCAAGACCCG TCACAGAAAA TTTAAAGAAA TTCAAGTCCC ATATAATGTC 
CAGTGGATGG CAATCTTCAG TGAACAACTC TGTGTGGGAT TCCAGTCAGG ATTTCTAAGA 
TACCCCTTGA ATGGAGAAGG AAATCCATAC AGTATGCTCC ATTCAAATGA CCATACACTA 
TCATTTATTG CACATCAACC AATGGATGCT ATCTGCGCAG TTGAGATCTC CAGTAAAGAA 
TATCTGCTGT GTTTTAACAG CATTGGGATA TACACTGACT GCCAGGGCCG AAGATCTAGA 
CAACAGGAAT TGATGTGGCC AGCAAATCCT TCCTCTTGTT GTTACAATGC ACCATATCTC 
TCGGTGTACA GTGAAAATGC AGTTGATATC TTTGATGTGA ACTCCATGGA ATGGATTCAG 
ACTCTTCCTC TCAAAAAGGT TCGACCCTTA AACAATGAAG GATCATTAAA TCTTTTAGGG 
TTGGAGACCA TTAGATTAAT ATATTTCAAA AATAAGATGG CAGAAGGGGA CGAACTGGTA 
GTACCTGAAA CATCAGATAA TAGTCGGAAA CAAATGGTTA GAAACATTAA CAATAAGCGG 
CGTTATTCCT TCAGAGTCCC AGAAGAGGAA AGGATGCAGC AGAGGAGGGA AATGCTACGA 
GATCCAGAAA TGAGAAATAA ATTAATTTCT AATCCAACTA ATTTTAATCA CATAGCACAC 
ATGGGTCCTG GAGATGGAAT ACAGATCCTG AAAGATCTGC CCATGCCAGG TTTCCCTTAT 
CCATCCCCTC ATCATCACTC CGGTCTGATC TCCTCTCCGA TCAACTTTGA ACACATCTAT 
CACATGACTG TTAATTCTGC TGAAAAATTT CTCTCTCCTG ATTCCATAAA CCCTGAATAT 
TCCCCGTCTC TGCGCTCTGT CCCTGGTACA CCAAGCTTTA TGACTCTGAG GAACCCTCGG 
CCTCAGGAAA GTCGGACAGT ATTCAGTGGC TCAGTCAGTA TTCCATCTAT CACCAAATCC 
CGCCCTGAGC CAGGCCGCTC CATGAGTGCT AGCAGTGGCT TGTCAGCAAG GTCATCCGCA 
CAGAATGGCA GCGCATTAAA GAGGGAATTC TCTGGAGGAA GCTACAGTGC CAAGCGGCAG 
CCCATGCCCT CCCCGTCAGA GGGCTCTTTG TCCTCTGGAG GCATGGACCA AGGAAGTGAT 
GCCCCAGCGA GGGACTTTGA CGGAGAGGAC TCTGACTCTC CGAGGCATTC CACAGCTTCC 
AACAGTTCCA ACCTAAGCAG CCCCCCAAGC CCAGCTTCAC CCCGAAAAAC CAAGAGCCTC 
TCCCTGGAGA GCACTGACCG CGGGAGCTGG GACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_005273318.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005273375.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X3, mRNA.

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CloneID	OHu35138
Clone ID Related Accession (Same CDS sequence)	XM_005273318.4 , XM_005273318.3
Accession Version	XM_005273318.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005273318.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGATC CCGTTGAGAA CACATATGTA TGGAACCCGA GCGTCAAGTT TCACATCCAG 
TCACGGTCCA CATCTCCTTC CACATCTAGT GAAGCTGAGC CAGTTAAGAC TGTAGACTCC 
ACTCCACTTT CAGTTCACAC ACCAACCTTA AGGAAAAAAG GATGTCCTGG TTCAACTGGC 
TTTCCACCTA AGCGCAAGAC TCACCAGTTT TTTGTAAAAT CTTTTACTAC TCCTACCAAG 
TGTCATCAGT GTACCTCCTT GATGGTGGGT TTAATAAGAC AGGGCTGTTC ATGTGAAGTG 
TGTGGATTCT CATGCCATAT AACTTGTGTA AACAAAGCTC CAACCACTTG TCCAGTTCCT 
CCTGAACAGA CAAAAGGTCC CCTGGGTATA GATCCTCAGA AAGGAATAGG AACAGCATAT 
GAAGGTCATG TCAGGATTCC TAAGCCAGCT GGAGTGAAGA AAGGGTGGCA GAGAGCACTG 
GCTATAGTGT GTGACTTCAA ACTCTTTCTG TACGATATTG CTGAAGGAAA AGCATCTCAG 
CCCAGTGTTG TCATTAGTCA AGTGATTGAC ATGAGGGATG AAGAATTTTC TGTGAGTTCA 
GTCTTGGCTT CTGATGTTAT CCATGCAAGT CGGAAAGATA TACCCTGTAT ATTTAGGGTC 
ACAGCTTCCC AGCTCTCAGC ATCTAATAAC AAATGTTCAA TCCTGATGCT AGCAGACACT 
GAGAATGAGA AGAATAAGTG GGTGGGAGTG CTGAGTGAAT TGCACAAGAT TTTGAAGAAA 
AACAAATTCA GAGACCGCTC AGTCTATGTT CCCAAAGAGG CTTATGACAG CACTCTACCC 
CTCATTAAAA CAACCCAGGC AGCCGCAATC ATAGATCATG AAAGAATTGC TTTGGGAAAC 
GAAGAAGGGT TATTTGTTGT ACATGTCACC AAAGATGAAA TTATTAGAGT TGGTGACAAT 
AAGAAGATTC ATCAGATTGA ACTCATTCCA AATGATCAGC TTGTTGCTGT GATCTCAGGA 
CGAAATCGTC ATGTACGACT TTTTCCTATG TCAGCATTGG ATGGGCGAGA GACCGATTTT 
TACAAGCTGT CAGAAACTAA AGGGTGTCAA ACCGTAACTT CTGGAAAGGT GCGCCATGGA 
GCTCTCACAT GCCTGTGTGT GGCTATGAAA AGGCAGGTCC TCTGTTATGA ACTATTTCAG 
AGCAAGACCC GTCACAGAAA ATTTAAAGAA ATTCAAGTCC CATATAATGT CCAGTGGATG 
GCAATCTTCA GTGAACAACT CTGTGTGGGA TTCCAGTCAG GATTTCTAAG ATACCCCTTG 
AATGGAGAAG GAAATCCATA CAGTATGCTC CATTCAAATG ACCATACACT ATCATTTATT 
GCACATCAAC CAATGGATGC TATCTGCGCA GTTGAGATCT CCAGTAAAGA ATATCTGCTG 
TGTTTTAACA GCATTGGGAT ATACACTGAC TGCCAGGGCC GAAGATCTAG ACAACAGGAA 
TTGATGTGGC CAGCAAATCC TTCCTCTTGT TGTTACAATG CACCATATCT CTCGGTGTAC 
AGTGAAAATG CAGTTGATAT CTTTGATGTG AACTCCATGG AATGGATTCA GACTCTTCCT 
CTCAAAAAGG TTCGACCCTT AAACAATGAA GGATCATTAA ATCTTTTAGG GTTGGAGACC 
ATTAGATTAA TATATTTCAA AAATAAGATG GCAGAAGGGG ACGAACTGGT AGTACCTGAA 
ACATCAGATA ATAGTCGGAA ACAAATGGTT AGAAACATTA ACAATAAGCG GCGTTATTCC 
TTCAGAGTCC CAGAAGAGGA AAGGATGCAG CAGAGGAGGG AAATGCTACG AGATCCAGAA 
ATGAGAAATA AATTAATTTC TAATCCAACT AATTTTAATC ACATAGCACA CATGGGTCCT 
GGAGATGGAA TACAGATCCT GAAAGATCTG CCCATGCCAG GTTTCCCTTA TCCATCCCCT 
CATCATCACT CCGGTCTGAT CTCCTCTCCG ATCAACTTTG AACACATCTA TCACATGACT 
GTTAATTCTG CTGAAAAATT TCTCTCTCCT GATTCCATAA ACCCTGAATA TTCCCCGTCT 
CTGCGCTCTG TCCCTGGTAC ACCAAGCTTT ATGACTCTGA GGAACCCTCG GCCTCAGGAA 
AGTCGGACAG TATTCAGTGG CTCAGTCAGT ATTCCATCTA TCACCAAATC CCGCCCTGAG 
CCAGGCCGCT CCATGAGTGC TAGCAGTGGC TTGTCAGCAA GGTCATCCGC ACAGAATGGC 
AGCGCATTAA AGAGGGAATT CTCTGGAGGA AGCTACAGTG CCAAGCGGCA GCCCATGCCC 
TCCCCGTCAG AGGGCTCTTT GTCCTCTGGA GGCATGGACC AAGGAAGTGA TGCCCCAGCG 
AGGGACTTTG ACGGAGAGGA CTCTGACTCT CCGAGGCATT CCACAGCTTC CAACAGTTCC 
AACCTAAGCA GCCCCCCAAG CCCAGCTTCA CCCCGAAAAA CCAAGAGCCT CTCCCTGGAG 
AGCACTGACC GCGGGAGCTG GGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005273375.1
CDS	969..6458
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRTDPVENTYVWNPSVKFHIQSRSTSPSTSSEAEPVKTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_005273318.4
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005273318.4

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGATC CCGTTGAGAA CACATATGTA TGGAACCCGA GCGTCAAGTT TCACATCCAG 
TCACGGTCCA CATCTCCTTC CACATCTAGT GAAGCTGAGC CAGTTAAGAC TGTAGACTCC 
ACTCCACTTT CAGTTCACAC ACCAACCTTA AGGAAAAAAG GATGTCCTGG TTCAACTGGC 
TTTCCACCTA AGCGCAAGAC TCACCAGTTT TTTGTAAAAT CTTTTACTAC TCCTACCAAG 
TGTCATCAGT GTACCTCCTT GATGGTGGGT TTAATAAGAC AGGGCTGTTC ATGTGAAGTG 
TGTGGATTCT CATGCCATAT AACTTGTGTA AACAAAGCTC CAACCACTTG TCCAGTTCCT 
CCTGAACAGA CAAAAGGTCC CCTGGGTATA GATCCTCAGA AAGGAATAGG AACAGCATAT 
GAAGGTCATG TCAGGATTCC TAAGCCAGCT GGAGTGAAGA AAGGGTGGCA GAGAGCACTG 
GCTATAGTGT GTGACTTCAA ACTCTTTCTG TACGATATTG CTGAAGGAAA AGCATCTCAG 
CCCAGTGTTG TCATTAGTCA AGTGATTGAC ATGAGGGATG AAGAATTTTC TGTGAGTTCA 
GTCTTGGCTT CTGATGTTAT CCATGCAAGT CGGAAAGATA TACCCTGTAT ATTTAGGGTC 
ACAGCTTCCC AGCTCTCAGC ATCTAATAAC AAATGTTCAA TCCTGATGCT AGCAGACACT 
GAGAATGAGA AGAATAAGTG GGTGGGAGTG CTGAGTGAAT TGCACAAGAT TTTGAAGAAA 
AACAAATTCA GAGACCGCTC AGTCTATGTT CCCAAAGAGG CTTATGACAG CACTCTACCC 
CTCATTAAAA CAACCCAGGC AGCCGCAATC ATAGATCATG AAAGAATTGC TTTGGGAAAC 
GAAGAAGGGT TATTTGTTGT ACATGTCACC AAAGATGAAA TTATTAGAGT TGGTGACAAT 
AAGAAGATTC ATCAGATTGA ACTCATTCCA AATGATCAGC TTGTTGCTGT GATCTCAGGA 
CGAAATCGTC ATGTACGACT TTTTCCTATG TCAGCATTGG ATGGGCGAGA GACCGATTTT 
TACAAGCTGT CAGAAACTAA AGGGTGTCAA ACCGTAACTT CTGGAAAGGT GCGCCATGGA 
GCTCTCACAT GCCTGTGTGT GGCTATGAAA AGGCAGGTCC TCTGTTATGA ACTATTTCAG 
AGCAAGACCC GTCACAGAAA ATTTAAAGAA ATTCAAGTCC CATATAATGT CCAGTGGATG 
GCAATCTTCA GTGAACAACT CTGTGTGGGA TTCCAGTCAG GATTTCTAAG ATACCCCTTG 
AATGGAGAAG GAAATCCATA CAGTATGCTC CATTCAAATG ACCATACACT ATCATTTATT 
GCACATCAAC CAATGGATGC TATCTGCGCA GTTGAGATCT CCAGTAAAGA ATATCTGCTG 
TGTTTTAACA GCATTGGGAT ATACACTGAC TGCCAGGGCC GAAGATCTAG ACAACAGGAA 
TTGATGTGGC CAGCAAATCC TTCCTCTTGT TGTTACAATG CACCATATCT CTCGGTGTAC 
AGTGAAAATG CAGTTGATAT CTTTGATGTG AACTCCATGG AATGGATTCA GACTCTTCCT 
CTCAAAAAGG TTCGACCCTT AAACAATGAA GGATCATTAA ATCTTTTAGG GTTGGAGACC 
ATTAGATTAA TATATTTCAA AAATAAGATG GCAGAAGGGG ACGAACTGGT AGTACCTGAA 
ACATCAGATA ATAGTCGGAA ACAAATGGTT AGAAACATTA ACAATAAGCG GCGTTATTCC 
TTCAGAGTCC CAGAAGAGGA AAGGATGCAG CAGAGGAGGG AAATGCTACG AGATCCAGAA 
ATGAGAAATA AATTAATTTC TAATCCAACT AATTTTAATC ACATAGCACA CATGGGTCCT 
GGAGATGGAA TACAGATCCT GAAAGATCTG CCCATGCCAG GTTTCCCTTA TCCATCCCCT 
CATCATCACT CCGGTCTGAT CTCCTCTCCG ATCAACTTTG AACACATCTA TCACATGACT 
GTTAATTCTG CTGAAAAATT TCTCTCTCCT GATTCCATAA ACCCTGAATA TTCCCCGTCT 
CTGCGCTCTG TCCCTGGTAC ACCAAGCTTT ATGACTCTGA GGAACCCTCG GCCTCAGGAA 
AGTCGGACAG TATTCAGTGG CTCAGTCAGT ATTCCATCTA TCACCAAATC CCGCCCTGAG 
CCAGGCCGCT CCATGAGTGC TAGCAGTGGC TTGTCAGCAA GGTCATCCGC ACAGAATGGC 
AGCGCATTAA AGAGGGAATT CTCTGGAGGA AGCTACAGTG CCAAGCGGCA GCCCATGCCC 
TCCCCGTCAG AGGGCTCTTT GTCCTCTGGA GGCATGGACC AAGGAAGTGA TGCCCCAGCG 
AGGGACTTTG ACGGAGAGGA CTCTGACTCT CCGAGGCATT CCACAGCTTC CAACAGTTCC 
AACCTAAGCA GCCCCCCAAG CCCAGCTTCA CCCCGAAAAA CCAAGAGCCT CTCCCTGGAG 
AGCACTGACC GCGGGAGCTG GGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_005273318.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005273375.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X3, mRNA.

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CloneID	OHu35138
Clone ID Related Accession (Same CDS sequence)	XM_005273318.4 , XM_005273318.3
Accession Version	XM_005273318.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005273318.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGATC CCGTTGAGAA CACATATGTA TGGAACCCGA GCGTCAAGTT TCACATCCAG 
TCACGGTCCA CATCTCCTTC CACATCTAGT GAAGCTGAGC CAGTTAAGAC TGTAGACTCC 
ACTCCACTTT CAGTTCACAC ACCAACCTTA AGGAAAAAAG GATGTCCTGG TTCAACTGGC 
TTTCCACCTA AGCGCAAGAC TCACCAGTTT TTTGTAAAAT CTTTTACTAC TCCTACCAAG 
TGTCATCAGT GTACCTCCTT GATGGTGGGT TTAATAAGAC AGGGCTGTTC ATGTGAAGTG 
TGTGGATTCT CATGCCATAT AACTTGTGTA AACAAAGCTC CAACCACTTG TCCAGTTCCT 
CCTGAACAGA CAAAAGGTCC CCTGGGTATA GATCCTCAGA AAGGAATAGG AACAGCATAT 
GAAGGTCATG TCAGGATTCC TAAGCCAGCT GGAGTGAAGA AAGGGTGGCA GAGAGCACTG 
GCTATAGTGT GTGACTTCAA ACTCTTTCTG TACGATATTG CTGAAGGAAA AGCATCTCAG 
CCCAGTGTTG TCATTAGTCA AGTGATTGAC ATGAGGGATG AAGAATTTTC TGTGAGTTCA 
GTCTTGGCTT CTGATGTTAT CCATGCAAGT CGGAAAGATA TACCCTGTAT ATTTAGGGTC 
ACAGCTTCCC AGCTCTCAGC ATCTAATAAC AAATGTTCAA TCCTGATGCT AGCAGACACT 
GAGAATGAGA AGAATAAGTG GGTGGGAGTG CTGAGTGAAT TGCACAAGAT TTTGAAGAAA 
AACAAATTCA GAGACCGCTC AGTCTATGTT CCCAAAGAGG CTTATGACAG CACTCTACCC 
CTCATTAAAA CAACCCAGGC AGCCGCAATC ATAGATCATG AAAGAATTGC TTTGGGAAAC 
GAAGAAGGGT TATTTGTTGT ACATGTCACC AAAGATGAAA TTATTAGAGT TGGTGACAAT 
AAGAAGATTC ATCAGATTGA ACTCATTCCA AATGATCAGC TTGTTGCTGT GATCTCAGGA 
CGAAATCGTC ATGTACGACT TTTTCCTATG TCAGCATTGG ATGGGCGAGA GACCGATTTT 
TACAAGCTGT CAGAAACTAA AGGGTGTCAA ACCGTAACTT CTGGAAAGGT GCGCCATGGA 
GCTCTCACAT GCCTGTGTGT GGCTATGAAA AGGCAGGTCC TCTGTTATGA ACTATTTCAG 
AGCAAGACCC GTCACAGAAA ATTTAAAGAA ATTCAAGTCC CATATAATGT CCAGTGGATG 
GCAATCTTCA GTGAACAACT CTGTGTGGGA TTCCAGTCAG GATTTCTAAG ATACCCCTTG 
AATGGAGAAG GAAATCCATA CAGTATGCTC CATTCAAATG ACCATACACT ATCATTTATT 
GCACATCAAC CAATGGATGC TATCTGCGCA GTTGAGATCT CCAGTAAAGA ATATCTGCTG 
TGTTTTAACA GCATTGGGAT ATACACTGAC TGCCAGGGCC GAAGATCTAG ACAACAGGAA 
TTGATGTGGC CAGCAAATCC TTCCTCTTGT TGTTACAATG CACCATATCT CTCGGTGTAC 
AGTGAAAATG CAGTTGATAT CTTTGATGTG AACTCCATGG AATGGATTCA GACTCTTCCT 
CTCAAAAAGG TTCGACCCTT AAACAATGAA GGATCATTAA ATCTTTTAGG GTTGGAGACC 
ATTAGATTAA TATATTTCAA AAATAAGATG GCAGAAGGGG ACGAACTGGT AGTACCTGAA 
ACATCAGATA ATAGTCGGAA ACAAATGGTT AGAAACATTA ACAATAAGCG GCGTTATTCC 
TTCAGAGTCC CAGAAGAGGA AAGGATGCAG CAGAGGAGGG AAATGCTACG AGATCCAGAA 
ATGAGAAATA AATTAATTTC TAATCCAACT AATTTTAATC ACATAGCACA CATGGGTCCT 
GGAGATGGAA TACAGATCCT GAAAGATCTG CCCATGCCAG GTTTCCCTTA TCCATCCCCT 
CATCATCACT CCGGTCTGAT CTCCTCTCCG ATCAACTTTG AACACATCTA TCACATGACT 
GTTAATTCTG CTGAAAAATT TCTCTCTCCT GATTCCATAA ACCCTGAATA TTCCCCGTCT 
CTGCGCTCTG TCCCTGGTAC ACCAAGCTTT ATGACTCTGA GGAACCCTCG GCCTCAGGAA 
AGTCGGACAG TATTCAGTGG CTCAGTCAGT ATTCCATCTA TCACCAAATC CCGCCCTGAG 
CCAGGCCGCT CCATGAGTGC TAGCAGTGGC TTGTCAGCAA GGTCATCCGC ACAGAATGGC 
AGCGCATTAA AGAGGGAATT CTCTGGAGGA AGCTACAGTG CCAAGCGGCA GCCCATGCCC 
TCCCCGTCAG AGGGCTCTTT GTCCTCTGGA GGCATGGACC AAGGAAGTGA TGCCCCAGCG 
AGGGACTTTG ACGGAGAGGA CTCTGACTCT CCGAGGCATT CCACAGCTTC CAACAGTTCC 
AACCTAAGCA GCCCCCCAAG CCCAGCTTCA CCCCGAAAAA CCAAGAGCCT CTCCCTGGAG 
AGCACTGACC GCGGGAGCTG GGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005273375.1
CDS	1056..6545
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRTDPVENTYVWNPSVKFHIQSRSTSPSTSSEAEPVKTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_005273318.3
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005273318.3

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGATC CCGTTGAGAA CACATATGTA TGGAACCCGA GCGTCAAGTT TCACATCCAG 
TCACGGTCCA CATCTCCTTC CACATCTAGT GAAGCTGAGC CAGTTAAGAC TGTAGACTCC 
ACTCCACTTT CAGTTCACAC ACCAACCTTA AGGAAAAAAG GATGTCCTGG TTCAACTGGC 
TTTCCACCTA AGCGCAAGAC TCACCAGTTT TTTGTAAAAT CTTTTACTAC TCCTACCAAG 
TGTCATCAGT GTACCTCCTT GATGGTGGGT TTAATAAGAC AGGGCTGTTC ATGTGAAGTG 
TGTGGATTCT CATGCCATAT AACTTGTGTA AACAAAGCTC CAACCACTTG TCCAGTTCCT 
CCTGAACAGA CAAAAGGTCC CCTGGGTATA GATCCTCAGA AAGGAATAGG AACAGCATAT 
GAAGGTCATG TCAGGATTCC TAAGCCAGCT GGAGTGAAGA AAGGGTGGCA GAGAGCACTG 
GCTATAGTGT GTGACTTCAA ACTCTTTCTG TACGATATTG CTGAAGGAAA AGCATCTCAG 
CCCAGTGTTG TCATTAGTCA AGTGATTGAC ATGAGGGATG AAGAATTTTC TGTGAGTTCA 
GTCTTGGCTT CTGATGTTAT CCATGCAAGT CGGAAAGATA TACCCTGTAT ATTTAGGGTC 
ACAGCTTCCC AGCTCTCAGC ATCTAATAAC AAATGTTCAA TCCTGATGCT AGCAGACACT 
GAGAATGAGA AGAATAAGTG GGTGGGAGTG CTGAGTGAAT TGCACAAGAT TTTGAAGAAA 
AACAAATTCA GAGACCGCTC AGTCTATGTT CCCAAAGAGG CTTATGACAG CACTCTACCC 
CTCATTAAAA CAACCCAGGC AGCCGCAATC ATAGATCATG AAAGAATTGC TTTGGGAAAC 
GAAGAAGGGT TATTTGTTGT ACATGTCACC AAAGATGAAA TTATTAGAGT TGGTGACAAT 
AAGAAGATTC ATCAGATTGA ACTCATTCCA AATGATCAGC TTGTTGCTGT GATCTCAGGA 
CGAAATCGTC ATGTACGACT TTTTCCTATG TCAGCATTGG ATGGGCGAGA GACCGATTTT 
TACAAGCTGT CAGAAACTAA AGGGTGTCAA ACCGTAACTT CTGGAAAGGT GCGCCATGGA 
GCTCTCACAT GCCTGTGTGT GGCTATGAAA AGGCAGGTCC TCTGTTATGA ACTATTTCAG 
AGCAAGACCC GTCACAGAAA ATTTAAAGAA ATTCAAGTCC CATATAATGT CCAGTGGATG 
GCAATCTTCA GTGAACAACT CTGTGTGGGA TTCCAGTCAG GATTTCTAAG ATACCCCTTG 
AATGGAGAAG GAAATCCATA CAGTATGCTC CATTCAAATG ACCATACACT ATCATTTATT 
GCACATCAAC CAATGGATGC TATCTGCGCA GTTGAGATCT CCAGTAAAGA ATATCTGCTG 
TGTTTTAACA GCATTGGGAT ATACACTGAC TGCCAGGGCC GAAGATCTAG ACAACAGGAA 
TTGATGTGGC CAGCAAATCC TTCCTCTTGT TGTTACAATG CACCATATCT CTCGGTGTAC 
AGTGAAAATG CAGTTGATAT CTTTGATGTG AACTCCATGG AATGGATTCA GACTCTTCCT 
CTCAAAAAGG TTCGACCCTT AAACAATGAA GGATCATTAA ATCTTTTAGG GTTGGAGACC 
ATTAGATTAA TATATTTCAA AAATAAGATG GCAGAAGGGG ACGAACTGGT AGTACCTGAA 
ACATCAGATA ATAGTCGGAA ACAAATGGTT AGAAACATTA ACAATAAGCG GCGTTATTCC 
TTCAGAGTCC CAGAAGAGGA AAGGATGCAG CAGAGGAGGG AAATGCTACG AGATCCAGAA 
ATGAGAAATA AATTAATTTC TAATCCAACT AATTTTAATC ACATAGCACA CATGGGTCCT 
GGAGATGGAA TACAGATCCT GAAAGATCTG CCCATGCCAG GTTTCCCTTA TCCATCCCCT 
CATCATCACT CCGGTCTGAT CTCCTCTCCG ATCAACTTTG AACACATCTA TCACATGACT 
GTTAATTCTG CTGAAAAATT TCTCTCTCCT GATTCCATAA ACCCTGAATA TTCCCCGTCT 
CTGCGCTCTG TCCCTGGTAC ACCAAGCTTT ATGACTCTGA GGAACCCTCG GCCTCAGGAA 
AGTCGGACAG TATTCAGTGG CTCAGTCAGT ATTCCATCTA TCACCAAATC CCGCCCTGAG 
CCAGGCCGCT CCATGAGTGC TAGCAGTGGC TTGTCAGCAA GGTCATCCGC ACAGAATGGC 
AGCGCATTAA AGAGGGAATT CTCTGGAGGA AGCTACAGTG CCAAGCGGCA GCCCATGCCC 
TCCCCGTCAG AGGGCTCTTT GTCCTCTGGA GGCATGGACC AAGGAAGTGA TGCCCCAGCG 
AGGGACTTTG ACGGAGAGGA CTCTGACTCT CCGAGGCATT CCACAGCTTC CAACAGTTCC 
AACCTAAGCA GCCCCCCAAG CCCAGCTTCA CCCCGAAAAA CCAAGAGCCT CTCCCTGGAG 
AGCACTGACC GCGGGAGCTG GGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006711834.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006711897.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X4, mRNA.

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CloneID	OHu35139
Clone ID Related Accession (Same CDS sequence)	XM_006711834.3 , XM_006711834.4
Accession Version	XM_006711834.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006711834.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGAT CCCGTTGAGA ACACATATGT ATGGAACCCG 
AGCGTCAAGT TTCACATCCA GTCACGGTCC ACATCTCCTT CCACATCTAG TGAAGCTGAG 
CCAGTTAAGA CTGTAGACTC CACTCCACTT TCAGTTCACA CACCAACCTT AAGGAAAAAA 
GGATGTCCTG GTTCAACTGG CTTTCCACCT AAGCGCAAGA CTCACCAGTT TTTTGTAAAA 
TCTTTTACTA CTCCTACCAA GTGTCATCAG TGTACCTCCT TGATGGTGGG TTTAATAAGA 
CAGGGCTGTT CATGTGAAGT GTGTGGATTC TCATGCCATA TAACTTGTGT AAACAAAGCT 
CCAACCACTT GTCCAGTTCC TCCTGAACAG ACAAAAGGTC CCCTGGGTAT AGATCCTCAG 
AAAGGAATAG GAACAGCATA TGAAGGTCAT GTCAGGATTC CTAAGCCAGC TGGAGTGAAG 
AAAGGGTGGC AGAGAGCACT GGCTATAGTG TGTGACTTCA AACTCTTTCT GTACGATATT 
GCTGAAGGAA AAGCATCTCA GCCCAGTGTT GTCATTAGTC AAGTGATTGA CATGAGGGAT 
GAAGAATTTT CTGTGAGTTC AGTCTTGGCT TCTGATGTTA TCCATGCAAG TCGGAAAGAT 
ATACCCTGTA TATTTAGGGT CACAGCTTCC CAGCTCTCAG CATCTAATAA CAAATGTTCA 
ATCCTGATGC TAGCAGACAC TGAGAATGAG AAGAATAAGT GGGTGGGAGT GCTGAGTGAA 
TTGCACAAGA TTTTGAAGAA AAACAAATTC AGAGACCGCT CAGTCTATGT TCCCAAAGAG 
GCTTATGACA GCACTCTACC CCTCATTAAA ACAACCCAGG CAGCCGCAAT CATAGATCAT 
GAAAGAATTG CTTTGGGAAA CGAAGAAGGG TTATTTGTTG TACATGTCAC CAAAGATGAA 
ATTATTAGAG TTGGTGACAA TAAGAAGATT CATCAGATTG AACTCATTCC AAATGATCAG 
CTTGTTGCTG TGATCTCAGG ACGAAATCGT CATGTACGAC TTTTTCCTAT GTCAGCATTG 
GATGGGCGAG AGACCGATTT TTACAAGCTG TCAGAAACTA AAGGGTGTCA AACCGTAACT 
TCTGGAAAGG TGCGCCATGG AGCTCTCACA TGCCTGTGTG TGGCTATGAA AAGGCAGGTC 
CTCTGTTATG AACTATTTCA GAGCAAGACC CGTCACAGAA AATTTAAAGA AATTCAAGTC 
CCATATAATG TCCAGTGGAT GGCAATCTTC AGTGAACAAC TCTGTGTGGG ATTCCAGTCA 
GGATTTCTAA GATACCCCTT GAATGGAGAA GGAAATCCAT ACAGTATGCT CCATTCAAAT 
GACCATACAC TATCATTTAT TGCACATCAA CCAATGGATG CTATCTGCGC AGTTGAGATC 
TCCAGTAAAG AATATCTGCT GTGTTTTAAC AGCATTGGGA TATACACTGA CTGCCAGGGC 
CGAAGATCTA GACAACAGGA ATTGATGTGG CCAGCAAATC CTTCCTCTTG TTGTTACAAT 
GCACCATATC TCTCGGTGTA CAGTGAAAAT GCAGTTGATA TCTTTGATGT GAACTCCATG 
GAATGGATTC AGACTCTTCC TCTCAAAAAG GTTCGACCCT TAAACAATGA AGGATCATTA 
AATCTTTTAG GGTTGGAGAC CATTAGATTA ATATATTTCA AAAATAAGAT GGCAGAAGGG 
GACGAACTGG TAGTACCTGA AACATCAGAT AATAGTCGGA AACAAATGGT TAGAAACATT 
AACAATAAGC GGCGTTATTC CTTCAGAGTC CCAGAAGAGG AAAGGATGCA GCAGAGGAGG 
GAAATGCTAC GAGATCCAGA AATGAGAAAT AAATTAATTT CTAATCCAAC TAATTTTAAT 
CACATAGCAC ACATGGGTCC TGGAGATGGA ATACAGATCC TGAAAGATCT GCCCATGCCA 
GGTTTCCCTT ATCCATCCCC TCATCATCAC TCCGGTCTGA TCTCCTCTCC GATCAACTTT 
GAACACATCT ATCACATGAC TGTTAATTCT GCTGAAAAAT TTCTCTCTCC TGATTCCATA 
AACCCTGAAT ATTCCCCGTC TCTGCGCTCT GTCCCTGGTA CACCAAGCTT TATGACTCTG 
AGGAACCCTC GGCCTCAGGA AAGTCGGACA GTATTCAGTG GCTCAGTCAG TATTCCATCT 
ATCACCAAAT CCCGCCCTGA GCCAGGCCGC TCCATGAGTG CTAGCAGTGG CTTGTCAGCA 
AGGTCATCCG CACAGAATGG CAGCGCATTA AAGAGGGAAT TCTCTGGAGG AAGCTACAGT 
GCCAAGCGGC AGCCCATGCC CTCCCCGTCA GAGGGCTCTT TGTCCTCTGG AGGCATGGAC 
CAAGGAAGTG ATGCCCCAGC GAGGGACTTT GACGGAGAGG ACTCTGACTC TCCGAGGCAT 
TCCACAGCTT CCAACAGTTC CAACCTAAGC AGCCCCCCAA GCCCAGCTTC ACCCCGAAAA 
ACCAAGAGCC TCTCCCTGGA GAGCACTGAC CGCGGGAGCT GGGACCCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006711897.1
CDS	1056..6506
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETDPVENTYVWNPSVKFHIQSRSTSPSTSSEAEPVKTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006711834.3
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006711834.3

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGAT CCCGTTGAGA ACACATATGT ATGGAACCCG 
AGCGTCAAGT TTCACATCCA GTCACGGTCC ACATCTCCTT CCACATCTAG TGAAGCTGAG 
CCAGTTAAGA CTGTAGACTC CACTCCACTT TCAGTTCACA CACCAACCTT AAGGAAAAAA 
GGATGTCCTG GTTCAACTGG CTTTCCACCT AAGCGCAAGA CTCACCAGTT TTTTGTAAAA 
TCTTTTACTA CTCCTACCAA GTGTCATCAG TGTACCTCCT TGATGGTGGG TTTAATAAGA 
CAGGGCTGTT CATGTGAAGT GTGTGGATTC TCATGCCATA TAACTTGTGT AAACAAAGCT 
CCAACCACTT GTCCAGTTCC TCCTGAACAG ACAAAAGGTC CCCTGGGTAT AGATCCTCAG 
AAAGGAATAG GAACAGCATA TGAAGGTCAT GTCAGGATTC CTAAGCCAGC TGGAGTGAAG 
AAAGGGTGGC AGAGAGCACT GGCTATAGTG TGTGACTTCA AACTCTTTCT GTACGATATT 
GCTGAAGGAA AAGCATCTCA GCCCAGTGTT GTCATTAGTC AAGTGATTGA CATGAGGGAT 
GAAGAATTTT CTGTGAGTTC AGTCTTGGCT TCTGATGTTA TCCATGCAAG TCGGAAAGAT 
ATACCCTGTA TATTTAGGGT CACAGCTTCC CAGCTCTCAG CATCTAATAA CAAATGTTCA 
ATCCTGATGC TAGCAGACAC TGAGAATGAG AAGAATAAGT GGGTGGGAGT GCTGAGTGAA 
TTGCACAAGA TTTTGAAGAA AAACAAATTC AGAGACCGCT CAGTCTATGT TCCCAAAGAG 
GCTTATGACA GCACTCTACC CCTCATTAAA ACAACCCAGG CAGCCGCAAT CATAGATCAT 
GAAAGAATTG CTTTGGGAAA CGAAGAAGGG TTATTTGTTG TACATGTCAC CAAAGATGAA 
ATTATTAGAG TTGGTGACAA TAAGAAGATT CATCAGATTG AACTCATTCC AAATGATCAG 
CTTGTTGCTG TGATCTCAGG ACGAAATCGT CATGTACGAC TTTTTCCTAT GTCAGCATTG 
GATGGGCGAG AGACCGATTT TTACAAGCTG TCAGAAACTA AAGGGTGTCA AACCGTAACT 
TCTGGAAAGG TGCGCCATGG AGCTCTCACA TGCCTGTGTG TGGCTATGAA AAGGCAGGTC 
CTCTGTTATG AACTATTTCA GAGCAAGACC CGTCACAGAA AATTTAAAGA AATTCAAGTC 
CCATATAATG TCCAGTGGAT GGCAATCTTC AGTGAACAAC TCTGTGTGGG ATTCCAGTCA 
GGATTTCTAA GATACCCCTT GAATGGAGAA GGAAATCCAT ACAGTATGCT CCATTCAAAT 
GACCATACAC TATCATTTAT TGCACATCAA CCAATGGATG CTATCTGCGC AGTTGAGATC 
TCCAGTAAAG AATATCTGCT GTGTTTTAAC AGCATTGGGA TATACACTGA CTGCCAGGGC 
CGAAGATCTA GACAACAGGA ATTGATGTGG CCAGCAAATC CTTCCTCTTG TTGTTACAAT 
GCACCATATC TCTCGGTGTA CAGTGAAAAT GCAGTTGATA TCTTTGATGT GAACTCCATG 
GAATGGATTC AGACTCTTCC TCTCAAAAAG GTTCGACCCT TAAACAATGA AGGATCATTA 
AATCTTTTAG GGTTGGAGAC CATTAGATTA ATATATTTCA AAAATAAGAT GGCAGAAGGG 
GACGAACTGG TAGTACCTGA AACATCAGAT AATAGTCGGA AACAAATGGT TAGAAACATT 
AACAATAAGC GGCGTTATTC CTTCAGAGTC CCAGAAGAGG AAAGGATGCA GCAGAGGAGG 
GAAATGCTAC GAGATCCAGA AATGAGAAAT AAATTAATTT CTAATCCAAC TAATTTTAAT 
CACATAGCAC ACATGGGTCC TGGAGATGGA ATACAGATCC TGAAAGATCT GCCCATGCCA 
GGTTTCCCTT ATCCATCCCC TCATCATCAC TCCGGTCTGA TCTCCTCTCC GATCAACTTT 
GAACACATCT ATCACATGAC TGTTAATTCT GCTGAAAAAT TTCTCTCTCC TGATTCCATA 
AACCCTGAAT ATTCCCCGTC TCTGCGCTCT GTCCCTGGTA CACCAAGCTT TATGACTCTG 
AGGAACCCTC GGCCTCAGGA AAGTCGGACA GTATTCAGTG GCTCAGTCAG TATTCCATCT 
ATCACCAAAT CCCGCCCTGA GCCAGGCCGC TCCATGAGTG CTAGCAGTGG CTTGTCAGCA 
AGGTCATCCG CACAGAATGG CAGCGCATTA AAGAGGGAAT TCTCTGGAGG AAGCTACAGT 
GCCAAGCGGC AGCCCATGCC CTCCCCGTCA GAGGGCTCTT TGTCCTCTGG AGGCATGGAC 
CAAGGAAGTG ATGCCCCAGC GAGGGACTTT GACGGAGAGG ACTCTGACTC TCCGAGGCAT 
TCCACAGCTT CCAACAGTTC CAACCTAAGC AGCCCCCCAA GCCCAGCTTC ACCCCGAAAA 
ACCAAGAGCC TCTCCCTGGA GAGCACTGAC CGCGGGAGCT GGGACCCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006711834.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006711897.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X4, mRNA.

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CloneID	OHu35139
Clone ID Related Accession (Same CDS sequence)	XM_006711834.3 , XM_006711834.4
Accession Version	XM_006711834.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006711834.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGAT CCCGTTGAGA ACACATATGT ATGGAACCCG 
AGCGTCAAGT TTCACATCCA GTCACGGTCC ACATCTCCTT CCACATCTAG TGAAGCTGAG 
CCAGTTAAGA CTGTAGACTC CACTCCACTT TCAGTTCACA CACCAACCTT AAGGAAAAAA 
GGATGTCCTG GTTCAACTGG CTTTCCACCT AAGCGCAAGA CTCACCAGTT TTTTGTAAAA 
TCTTTTACTA CTCCTACCAA GTGTCATCAG TGTACCTCCT TGATGGTGGG TTTAATAAGA 
CAGGGCTGTT CATGTGAAGT GTGTGGATTC TCATGCCATA TAACTTGTGT AAACAAAGCT 
CCAACCACTT GTCCAGTTCC TCCTGAACAG ACAAAAGGTC CCCTGGGTAT AGATCCTCAG 
AAAGGAATAG GAACAGCATA TGAAGGTCAT GTCAGGATTC CTAAGCCAGC TGGAGTGAAG 
AAAGGGTGGC AGAGAGCACT GGCTATAGTG TGTGACTTCA AACTCTTTCT GTACGATATT 
GCTGAAGGAA AAGCATCTCA GCCCAGTGTT GTCATTAGTC AAGTGATTGA CATGAGGGAT 
GAAGAATTTT CTGTGAGTTC AGTCTTGGCT TCTGATGTTA TCCATGCAAG TCGGAAAGAT 
ATACCCTGTA TATTTAGGGT CACAGCTTCC CAGCTCTCAG CATCTAATAA CAAATGTTCA 
ATCCTGATGC TAGCAGACAC TGAGAATGAG AAGAATAAGT GGGTGGGAGT GCTGAGTGAA 
TTGCACAAGA TTTTGAAGAA AAACAAATTC AGAGACCGCT CAGTCTATGT TCCCAAAGAG 
GCTTATGACA GCACTCTACC CCTCATTAAA ACAACCCAGG CAGCCGCAAT CATAGATCAT 
GAAAGAATTG CTTTGGGAAA CGAAGAAGGG TTATTTGTTG TACATGTCAC CAAAGATGAA 
ATTATTAGAG TTGGTGACAA TAAGAAGATT CATCAGATTG AACTCATTCC AAATGATCAG 
CTTGTTGCTG TGATCTCAGG ACGAAATCGT CATGTACGAC TTTTTCCTAT GTCAGCATTG 
GATGGGCGAG AGACCGATTT TTACAAGCTG TCAGAAACTA AAGGGTGTCA AACCGTAACT 
TCTGGAAAGG TGCGCCATGG AGCTCTCACA TGCCTGTGTG TGGCTATGAA AAGGCAGGTC 
CTCTGTTATG AACTATTTCA GAGCAAGACC CGTCACAGAA AATTTAAAGA AATTCAAGTC 
CCATATAATG TCCAGTGGAT GGCAATCTTC AGTGAACAAC TCTGTGTGGG ATTCCAGTCA 
GGATTTCTAA GATACCCCTT GAATGGAGAA GGAAATCCAT ACAGTATGCT CCATTCAAAT 
GACCATACAC TATCATTTAT TGCACATCAA CCAATGGATG CTATCTGCGC AGTTGAGATC 
TCCAGTAAAG AATATCTGCT GTGTTTTAAC AGCATTGGGA TATACACTGA CTGCCAGGGC 
CGAAGATCTA GACAACAGGA ATTGATGTGG CCAGCAAATC CTTCCTCTTG TTGTTACAAT 
GCACCATATC TCTCGGTGTA CAGTGAAAAT GCAGTTGATA TCTTTGATGT GAACTCCATG 
GAATGGATTC AGACTCTTCC TCTCAAAAAG GTTCGACCCT TAAACAATGA AGGATCATTA 
AATCTTTTAG GGTTGGAGAC CATTAGATTA ATATATTTCA AAAATAAGAT GGCAGAAGGG 
GACGAACTGG TAGTACCTGA AACATCAGAT AATAGTCGGA AACAAATGGT TAGAAACATT 
AACAATAAGC GGCGTTATTC CTTCAGAGTC CCAGAAGAGG AAAGGATGCA GCAGAGGAGG 
GAAATGCTAC GAGATCCAGA AATGAGAAAT AAATTAATTT CTAATCCAAC TAATTTTAAT 
CACATAGCAC ACATGGGTCC TGGAGATGGA ATACAGATCC TGAAAGATCT GCCCATGCCA 
GGTTTCCCTT ATCCATCCCC TCATCATCAC TCCGGTCTGA TCTCCTCTCC GATCAACTTT 
GAACACATCT ATCACATGAC TGTTAATTCT GCTGAAAAAT TTCTCTCTCC TGATTCCATA 
AACCCTGAAT ATTCCCCGTC TCTGCGCTCT GTCCCTGGTA CACCAAGCTT TATGACTCTG 
AGGAACCCTC GGCCTCAGGA AAGTCGGACA GTATTCAGTG GCTCAGTCAG TATTCCATCT 
ATCACCAAAT CCCGCCCTGA GCCAGGCCGC TCCATGAGTG CTAGCAGTGG CTTGTCAGCA 
AGGTCATCCG CACAGAATGG CAGCGCATTA AAGAGGGAAT TCTCTGGAGG AAGCTACAGT 
GCCAAGCGGC AGCCCATGCC CTCCCCGTCA GAGGGCTCTT TGTCCTCTGG AGGCATGGAC 
CAAGGAAGTG ATGCCCCAGC GAGGGACTTT GACGGAGAGG ACTCTGACTC TCCGAGGCAT 
TCCACAGCTT CCAACAGTTC CAACCTAAGC AGCCCCCCAA GCCCAGCTTC ACCCCGAAAA 
ACCAAGAGCC TCTCCCTGGA GAGCACTGAC CGCGGGAGCT GGGACCCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006711897.1
CDS	969..6419
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETDPVENTYVWNPSVKFHIQSRSTSPSTSSEAEPVKTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006711834.4
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006711834.4

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGAT CCCGTTGAGA ACACATATGT ATGGAACCCG 
AGCGTCAAGT TTCACATCCA GTCACGGTCC ACATCTCCTT CCACATCTAG TGAAGCTGAG 
CCAGTTAAGA CTGTAGACTC CACTCCACTT TCAGTTCACA CACCAACCTT AAGGAAAAAA 
GGATGTCCTG GTTCAACTGG CTTTCCACCT AAGCGCAAGA CTCACCAGTT TTTTGTAAAA 
TCTTTTACTA CTCCTACCAA GTGTCATCAG TGTACCTCCT TGATGGTGGG TTTAATAAGA 
CAGGGCTGTT CATGTGAAGT GTGTGGATTC TCATGCCATA TAACTTGTGT AAACAAAGCT 
CCAACCACTT GTCCAGTTCC TCCTGAACAG ACAAAAGGTC CCCTGGGTAT AGATCCTCAG 
AAAGGAATAG GAACAGCATA TGAAGGTCAT GTCAGGATTC CTAAGCCAGC TGGAGTGAAG 
AAAGGGTGGC AGAGAGCACT GGCTATAGTG TGTGACTTCA AACTCTTTCT GTACGATATT 
GCTGAAGGAA AAGCATCTCA GCCCAGTGTT GTCATTAGTC AAGTGATTGA CATGAGGGAT 
GAAGAATTTT CTGTGAGTTC AGTCTTGGCT TCTGATGTTA TCCATGCAAG TCGGAAAGAT 
ATACCCTGTA TATTTAGGGT CACAGCTTCC CAGCTCTCAG CATCTAATAA CAAATGTTCA 
ATCCTGATGC TAGCAGACAC TGAGAATGAG AAGAATAAGT GGGTGGGAGT GCTGAGTGAA 
TTGCACAAGA TTTTGAAGAA AAACAAATTC AGAGACCGCT CAGTCTATGT TCCCAAAGAG 
GCTTATGACA GCACTCTACC CCTCATTAAA ACAACCCAGG CAGCCGCAAT CATAGATCAT 
GAAAGAATTG CTTTGGGAAA CGAAGAAGGG TTATTTGTTG TACATGTCAC CAAAGATGAA 
ATTATTAGAG TTGGTGACAA TAAGAAGATT CATCAGATTG AACTCATTCC AAATGATCAG 
CTTGTTGCTG TGATCTCAGG ACGAAATCGT CATGTACGAC TTTTTCCTAT GTCAGCATTG 
GATGGGCGAG AGACCGATTT TTACAAGCTG TCAGAAACTA AAGGGTGTCA AACCGTAACT 
TCTGGAAAGG TGCGCCATGG AGCTCTCACA TGCCTGTGTG TGGCTATGAA AAGGCAGGTC 
CTCTGTTATG AACTATTTCA GAGCAAGACC CGTCACAGAA AATTTAAAGA AATTCAAGTC 
CCATATAATG TCCAGTGGAT GGCAATCTTC AGTGAACAAC TCTGTGTGGG ATTCCAGTCA 
GGATTTCTAA GATACCCCTT GAATGGAGAA GGAAATCCAT ACAGTATGCT CCATTCAAAT 
GACCATACAC TATCATTTAT TGCACATCAA CCAATGGATG CTATCTGCGC AGTTGAGATC 
TCCAGTAAAG AATATCTGCT GTGTTTTAAC AGCATTGGGA TATACACTGA CTGCCAGGGC 
CGAAGATCTA GACAACAGGA ATTGATGTGG CCAGCAAATC CTTCCTCTTG TTGTTACAAT 
GCACCATATC TCTCGGTGTA CAGTGAAAAT GCAGTTGATA TCTTTGATGT GAACTCCATG 
GAATGGATTC AGACTCTTCC TCTCAAAAAG GTTCGACCCT TAAACAATGA AGGATCATTA 
AATCTTTTAG GGTTGGAGAC CATTAGATTA ATATATTTCA AAAATAAGAT GGCAGAAGGG 
GACGAACTGG TAGTACCTGA AACATCAGAT AATAGTCGGA AACAAATGGT TAGAAACATT 
AACAATAAGC GGCGTTATTC CTTCAGAGTC CCAGAAGAGG AAAGGATGCA GCAGAGGAGG 
GAAATGCTAC GAGATCCAGA AATGAGAAAT AAATTAATTT CTAATCCAAC TAATTTTAAT 
CACATAGCAC ACATGGGTCC TGGAGATGGA ATACAGATCC TGAAAGATCT GCCCATGCCA 
GGTTTCCCTT ATCCATCCCC TCATCATCAC TCCGGTCTGA TCTCCTCTCC GATCAACTTT 
GAACACATCT ATCACATGAC TGTTAATTCT GCTGAAAAAT TTCTCTCTCC TGATTCCATA 
AACCCTGAAT ATTCCCCGTC TCTGCGCTCT GTCCCTGGTA CACCAAGCTT TATGACTCTG 
AGGAACCCTC GGCCTCAGGA AAGTCGGACA GTATTCAGTG GCTCAGTCAG TATTCCATCT 
ATCACCAAAT CCCGCCCTGA GCCAGGCCGC TCCATGAGTG CTAGCAGTGG CTTGTCAGCA 
AGGTCATCCG CACAGAATGG CAGCGCATTA AAGAGGGAAT TCTCTGGAGG AAGCTACAGT 
GCCAAGCGGC AGCCCATGCC CTCCCCGTCA GAGGGCTCTT TGTCCTCTGG AGGCATGGAC 
CAAGGAAGTG ATGCCCCAGC GAGGGACTTT GACGGAGAGG ACTCTGACTC TCCGAGGCAT 
TCCACAGCTT CCAACAGTTC CAACCTAAGC AGCCCCCCAA GCCCAGCTTC ACCCCGAAAA 
ACCAAGAGCC TCTCCCTGGA GAGCACTGAC CGCGGGAGCT GGGACCCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006711835.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006711898.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X6, mRNA.

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CloneID	OHu35140
Clone ID Related Accession (Same CDS sequence)	XM_006711835.3 , XM_006711835.4
Accession Version	XM_006711835.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006711835.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACACTGTAGA CTCCACTCCA CTTTCAGTTC ACACACCAAC CTTAAGGAAA 
AAAGGATGTC CTGGTTCAAC TGGCTTTCCA CCTAAGCGCA AGACTCACCA GTTTTTTGTA 
AAATCTTTTA CTACTCCTAC CAAGTGTCAT CAGTGTACCT CCTTGATGGT GGGTTTAATA 
AGACAGGGCT GTTCATGTGA AGTGTGTGGA TTCTCATGCC ATATAACTTG TGTAAACAAA 
GCTCCAACCA CTTGTCCAGT TCCTCCTGAA CAGACAAAAG GTCCCCTGGG TATAGATCCT 
CAGAAAGGAA TAGGAACAGC ATATGAAGGT CATGTCAGGA TTCCTAAGCC AGCTGGAGTG 
AAGAAAGGGT GGCAGAGAGC ACTGGCTATA GTGTGTGACT TCAAACTCTT TCTGTACGAT 
ATTGCTGAAG GAAAAGCATC TCAGCCCAGT GTTGTCATTA GTCAAGTGAT TGACATGAGG 
GATGAAGAAT TTTCTGTGAG TTCAGTCTTG GCTTCTGATG TTATCCATGC AAGTCGGAAA 
GATATACCCT GTATATTTAG GGTCACAGCT TCCCAGCTCT CAGCATCTAA TAACAAATGT 
TCAATCCTGA TGCTAGCAGA CACTGAGAAT GAGAAGAATA AGTGGGTGGG AGTGCTGAGT 
GAATTGCACA AGATTTTGAA GAAAAACAAA TTCAGAGACC GCTCAGTCTA TGTTCCCAAA 
GAGGCTTATG ACAGCACTCT ACCCCTCATT AAAACAACCC AGGCAGCCGC AATCATAGAT 
CATGAAAGAA TTGCTTTGGG AAACGAAGAA GGGTTATTTG TTGTACATGT CACCAAAGAT 
GAAATTATTA GAGTTGGTGA CAATAAGAAG ATTCATCAGA TTGAACTCAT TCCAAATGAT 
CAGCTTGTTG CTGTGATCTC AGGACGAAAT CGTCATGTAC GACTTTTTCC TATGTCAGCA 
TTGGATGGGC GAGAGACCGA TTTTTACAAG CTGTCAGAAA CTAAAGGGTG TCAAACCGTA 
ACTTCTGGAA AGGTGCGCCA TGGAGCTCTC ACATGCCTGT GTGTGGCTAT GAAAAGGCAG 
GTCCTCTGTT ATGAACTATT TCAGAGCAAG ACCCGTCACA GAAAATTTAA AGAAATTCAA 
GTCCCATATA ATGTCCAGTG GATGGCAATC TTCAGTGAAC AACTCTGTGT GGGATTCCAG 
TCAGGATTTC TAAGATACCC CTTGAATGGA GAAGGAAATC CATACAGTAT GCTCCATTCA 
AATGACCATA CACTATCATT TATTGCACAT CAACCAATGG ATGCTATCTG CGCAGTTGAG 
ATCTCCAGTA AAGAATATCT GCTGTGTTTT AACAGCATTG GGATATACAC TGACTGCCAG 
GGCCGAAGAT CTAGACAACA GGAATTGATG TGGCCAGCAA ATCCTTCCTC TTGTTGTTAC 
AATGCACCAT ATCTCTCGGT GTACAGTGAA AATGCAGTTG ATATCTTTGA TGTGAACTCC 
ATGGAATGGA TTCAGACTCT TCCTCTCAAA AAGGTTCGAC CCTTAAACAA TGAAGGATCA 
TTAAATCTTT TAGGGTTGGA GACCATTAGA TTAATATATT TCAAAAATAA GATGGCAGAA 
GGGGACGAAC TGGTAGTACC TGAAACATCA GATAATAGTC GGAAACAAAT GGTTAGAAAC 
ATTAACAATA AGCGGCGTTA TTCCTTCAGA GTCCCAGAAG AGGAAAGGAT GCAGCAGAGG 
AGGGAAATGC TACGAGATCC AGAAATGAGA AATAAATTAA TTTCTAATCC AACTAATTTT 
AATCACATAG CACACATGGG TCCTGGAGAT GGAATACAGA TCCTGAAAGA TCTGCCCATG 
CCAGGTTTCC CTTATCCATC CCCTCATCAT CACTCCGGTC TGATCTCCTC TCCGATCAAC 
TTTGAACACA TCTATCACAT GACTGTTAAT TCTGCTGAAA AATTTCTCTC TCCTGATTCC 
ATAAACCCTG AATATTCCCC GTCTCTGCGC TCTGTCCCTG GTACACCAAG CTTTATGACT 
CTGAGGAACC CTCGGCCTCA GGAAAGTCGG ACAGTATTCA GTGGCTCAGT CAGTATTCCA 
TCTATCACCA AATCCCGCCC TGAGCCAGGC CGCTCCATGA GTGCTAGCAG TGGCTTGTCA 
GCAAGGTCAT CCGCACAGAA TGGCAGCGCA TTAAAGAGGG AATTCTCTGG AGGAAGCTAC 
AGTGCCAAGC GGCAGCCCAT GCCCTCCCCG TCAGAGGGCT CTTTGTCCTC TGGAGGCATG 
GACCAAGGAA GTGATGCCCC AGCGAGGGAC TTTGACGGAG AGGACTCTGA CTCTCCGAGG 
CATTCCACAG CTTCCAACAG TTCCAACCTA AGCAGCCCCC CAAGCCCAGC TTCACCCCGA 
AAAACCAAGA GCCTCTCCCT GGAGAGCACT GACCGCGGGA GCTGGGACCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006711898.1
CDS	1056..6449
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006711835.3
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006711835.3

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACACTGTAGA CTCCACTCCA CTTTCAGTTC ACACACCAAC CTTAAGGAAA 
AAAGGATGTC CTGGTTCAAC TGGCTTTCCA CCTAAGCGCA AGACTCACCA GTTTTTTGTA 
AAATCTTTTA CTACTCCTAC CAAGTGTCAT CAGTGTACCT CCTTGATGGT GGGTTTAATA 
AGACAGGGCT GTTCATGTGA AGTGTGTGGA TTCTCATGCC ATATAACTTG TGTAAACAAA 
GCTCCAACCA CTTGTCCAGT TCCTCCTGAA CAGACAAAAG GTCCCCTGGG TATAGATCCT 
CAGAAAGGAA TAGGAACAGC ATATGAAGGT CATGTCAGGA TTCCTAAGCC AGCTGGAGTG 
AAGAAAGGGT GGCAGAGAGC ACTGGCTATA GTGTGTGACT TCAAACTCTT TCTGTACGAT 
ATTGCTGAAG GAAAAGCATC TCAGCCCAGT GTTGTCATTA GTCAAGTGAT TGACATGAGG 
GATGAAGAAT TTTCTGTGAG TTCAGTCTTG GCTTCTGATG TTATCCATGC AAGTCGGAAA 
GATATACCCT GTATATTTAG GGTCACAGCT TCCCAGCTCT CAGCATCTAA TAACAAATGT 
TCAATCCTGA TGCTAGCAGA CACTGAGAAT GAGAAGAATA AGTGGGTGGG AGTGCTGAGT 
GAATTGCACA AGATTTTGAA GAAAAACAAA TTCAGAGACC GCTCAGTCTA TGTTCCCAAA 
GAGGCTTATG ACAGCACTCT ACCCCTCATT AAAACAACCC AGGCAGCCGC AATCATAGAT 
CATGAAAGAA TTGCTTTGGG AAACGAAGAA GGGTTATTTG TTGTACATGT CACCAAAGAT 
GAAATTATTA GAGTTGGTGA CAATAAGAAG ATTCATCAGA TTGAACTCAT TCCAAATGAT 
CAGCTTGTTG CTGTGATCTC AGGACGAAAT CGTCATGTAC GACTTTTTCC TATGTCAGCA 
TTGGATGGGC GAGAGACCGA TTTTTACAAG CTGTCAGAAA CTAAAGGGTG TCAAACCGTA 
ACTTCTGGAA AGGTGCGCCA TGGAGCTCTC ACATGCCTGT GTGTGGCTAT GAAAAGGCAG 
GTCCTCTGTT ATGAACTATT TCAGAGCAAG ACCCGTCACA GAAAATTTAA AGAAATTCAA 
GTCCCATATA ATGTCCAGTG GATGGCAATC TTCAGTGAAC AACTCTGTGT GGGATTCCAG 
TCAGGATTTC TAAGATACCC CTTGAATGGA GAAGGAAATC CATACAGTAT GCTCCATTCA 
AATGACCATA CACTATCATT TATTGCACAT CAACCAATGG ATGCTATCTG CGCAGTTGAG 
ATCTCCAGTA AAGAATATCT GCTGTGTTTT AACAGCATTG GGATATACAC TGACTGCCAG 
GGCCGAAGAT CTAGACAACA GGAATTGATG TGGCCAGCAA ATCCTTCCTC TTGTTGTTAC 
AATGCACCAT ATCTCTCGGT GTACAGTGAA AATGCAGTTG ATATCTTTGA TGTGAACTCC 
ATGGAATGGA TTCAGACTCT TCCTCTCAAA AAGGTTCGAC CCTTAAACAA TGAAGGATCA 
TTAAATCTTT TAGGGTTGGA GACCATTAGA TTAATATATT TCAAAAATAA GATGGCAGAA 
GGGGACGAAC TGGTAGTACC TGAAACATCA GATAATAGTC GGAAACAAAT GGTTAGAAAC 
ATTAACAATA AGCGGCGTTA TTCCTTCAGA GTCCCAGAAG AGGAAAGGAT GCAGCAGAGG 
AGGGAAATGC TACGAGATCC AGAAATGAGA AATAAATTAA TTTCTAATCC AACTAATTTT 
AATCACATAG CACACATGGG TCCTGGAGAT GGAATACAGA TCCTGAAAGA TCTGCCCATG 
CCAGGTTTCC CTTATCCATC CCCTCATCAT CACTCCGGTC TGATCTCCTC TCCGATCAAC 
TTTGAACACA TCTATCACAT GACTGTTAAT TCTGCTGAAA AATTTCTCTC TCCTGATTCC 
ATAAACCCTG AATATTCCCC GTCTCTGCGC TCTGTCCCTG GTACACCAAG CTTTATGACT 
CTGAGGAACC CTCGGCCTCA GGAAAGTCGG ACAGTATTCA GTGGCTCAGT CAGTATTCCA 
TCTATCACCA AATCCCGCCC TGAGCCAGGC CGCTCCATGA GTGCTAGCAG TGGCTTGTCA 
GCAAGGTCAT CCGCACAGAA TGGCAGCGCA TTAAAGAGGG AATTCTCTGG AGGAAGCTAC 
AGTGCCAAGC GGCAGCCCAT GCCCTCCCCG TCAGAGGGCT CTTTGTCCTC TGGAGGCATG 
GACCAAGGAA GTGATGCCCC AGCGAGGGAC TTTGACGGAG AGGACTCTGA CTCTCCGAGG 
CATTCCACAG CTTCCAACAG TTCCAACCTA AGCAGCCCCC CAAGCCCAGC TTCACCCCGA 
AAAACCAAGA GCCTCTCCCT GGAGAGCACT GACCGCGGGA GCTGGGACCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006711835.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006711898.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X6, mRNA.

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CloneID	OHu35140
Clone ID Related Accession (Same CDS sequence)	XM_006711835.3 , XM_006711835.4
Accession Version	XM_006711835.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006711835.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACACTGTAGA CTCCACTCCA CTTTCAGTTC ACACACCAAC CTTAAGGAAA 
AAAGGATGTC CTGGTTCAAC TGGCTTTCCA CCTAAGCGCA AGACTCACCA GTTTTTTGTA 
AAATCTTTTA CTACTCCTAC CAAGTGTCAT CAGTGTACCT CCTTGATGGT GGGTTTAATA 
AGACAGGGCT GTTCATGTGA AGTGTGTGGA TTCTCATGCC ATATAACTTG TGTAAACAAA 
GCTCCAACCA CTTGTCCAGT TCCTCCTGAA CAGACAAAAG GTCCCCTGGG TATAGATCCT 
CAGAAAGGAA TAGGAACAGC ATATGAAGGT CATGTCAGGA TTCCTAAGCC AGCTGGAGTG 
AAGAAAGGGT GGCAGAGAGC ACTGGCTATA GTGTGTGACT TCAAACTCTT TCTGTACGAT 
ATTGCTGAAG GAAAAGCATC TCAGCCCAGT GTTGTCATTA GTCAAGTGAT TGACATGAGG 
GATGAAGAAT TTTCTGTGAG TTCAGTCTTG GCTTCTGATG TTATCCATGC AAGTCGGAAA 
GATATACCCT GTATATTTAG GGTCACAGCT TCCCAGCTCT CAGCATCTAA TAACAAATGT 
TCAATCCTGA TGCTAGCAGA CACTGAGAAT GAGAAGAATA AGTGGGTGGG AGTGCTGAGT 
GAATTGCACA AGATTTTGAA GAAAAACAAA TTCAGAGACC GCTCAGTCTA TGTTCCCAAA 
GAGGCTTATG ACAGCACTCT ACCCCTCATT AAAACAACCC AGGCAGCCGC AATCATAGAT 
CATGAAAGAA TTGCTTTGGG AAACGAAGAA GGGTTATTTG TTGTACATGT CACCAAAGAT 
GAAATTATTA GAGTTGGTGA CAATAAGAAG ATTCATCAGA TTGAACTCAT TCCAAATGAT 
CAGCTTGTTG CTGTGATCTC AGGACGAAAT CGTCATGTAC GACTTTTTCC TATGTCAGCA 
TTGGATGGGC GAGAGACCGA TTTTTACAAG CTGTCAGAAA CTAAAGGGTG TCAAACCGTA 
ACTTCTGGAA AGGTGCGCCA TGGAGCTCTC ACATGCCTGT GTGTGGCTAT GAAAAGGCAG 
GTCCTCTGTT ATGAACTATT TCAGAGCAAG ACCCGTCACA GAAAATTTAA AGAAATTCAA 
GTCCCATATA ATGTCCAGTG GATGGCAATC TTCAGTGAAC AACTCTGTGT GGGATTCCAG 
TCAGGATTTC TAAGATACCC CTTGAATGGA GAAGGAAATC CATACAGTAT GCTCCATTCA 
AATGACCATA CACTATCATT TATTGCACAT CAACCAATGG ATGCTATCTG CGCAGTTGAG 
ATCTCCAGTA AAGAATATCT GCTGTGTTTT AACAGCATTG GGATATACAC TGACTGCCAG 
GGCCGAAGAT CTAGACAACA GGAATTGATG TGGCCAGCAA ATCCTTCCTC TTGTTGTTAC 
AATGCACCAT ATCTCTCGGT GTACAGTGAA AATGCAGTTG ATATCTTTGA TGTGAACTCC 
ATGGAATGGA TTCAGACTCT TCCTCTCAAA AAGGTTCGAC CCTTAAACAA TGAAGGATCA 
TTAAATCTTT TAGGGTTGGA GACCATTAGA TTAATATATT TCAAAAATAA GATGGCAGAA 
GGGGACGAAC TGGTAGTACC TGAAACATCA GATAATAGTC GGAAACAAAT GGTTAGAAAC 
ATTAACAATA AGCGGCGTTA TTCCTTCAGA GTCCCAGAAG AGGAAAGGAT GCAGCAGAGG 
AGGGAAATGC TACGAGATCC AGAAATGAGA AATAAATTAA TTTCTAATCC AACTAATTTT 
AATCACATAG CACACATGGG TCCTGGAGAT GGAATACAGA TCCTGAAAGA TCTGCCCATG 
CCAGGTTTCC CTTATCCATC CCCTCATCAT CACTCCGGTC TGATCTCCTC TCCGATCAAC 
TTTGAACACA TCTATCACAT GACTGTTAAT TCTGCTGAAA AATTTCTCTC TCCTGATTCC 
ATAAACCCTG AATATTCCCC GTCTCTGCGC TCTGTCCCTG GTACACCAAG CTTTATGACT 
CTGAGGAACC CTCGGCCTCA GGAAAGTCGG ACAGTATTCA GTGGCTCAGT CAGTATTCCA 
TCTATCACCA AATCCCGCCC TGAGCCAGGC CGCTCCATGA GTGCTAGCAG TGGCTTGTCA 
GCAAGGTCAT CCGCACAGAA TGGCAGCGCA TTAAAGAGGG AATTCTCTGG AGGAAGCTAC 
AGTGCCAAGC GGCAGCCCAT GCCCTCCCCG TCAGAGGGCT CTTTGTCCTC TGGAGGCATG 
GACCAAGGAA GTGATGCCCC AGCGAGGGAC TTTGACGGAG AGGACTCTGA CTCTCCGAGG 
CATTCCACAG CTTCCAACAG TTCCAACCTA AGCAGCCCCC CAAGCCCAGC TTCACCCCGA 
AAAACCAAGA GCCTCTCCCT GGAGAGCACT GACCGCGGGA GCTGGGACCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006711898.1
CDS	969..6362
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006711835.4
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006711835.4

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACACTGTAGA CTCCACTCCA CTTTCAGTTC ACACACCAAC CTTAAGGAAA 
AAAGGATGTC CTGGTTCAAC TGGCTTTCCA CCTAAGCGCA AGACTCACCA GTTTTTTGTA 
AAATCTTTTA CTACTCCTAC CAAGTGTCAT CAGTGTACCT CCTTGATGGT GGGTTTAATA 
AGACAGGGCT GTTCATGTGA AGTGTGTGGA TTCTCATGCC ATATAACTTG TGTAAACAAA 
GCTCCAACCA CTTGTCCAGT TCCTCCTGAA CAGACAAAAG GTCCCCTGGG TATAGATCCT 
CAGAAAGGAA TAGGAACAGC ATATGAAGGT CATGTCAGGA TTCCTAAGCC AGCTGGAGTG 
AAGAAAGGGT GGCAGAGAGC ACTGGCTATA GTGTGTGACT TCAAACTCTT TCTGTACGAT 
ATTGCTGAAG GAAAAGCATC TCAGCCCAGT GTTGTCATTA GTCAAGTGAT TGACATGAGG 
GATGAAGAAT TTTCTGTGAG TTCAGTCTTG GCTTCTGATG TTATCCATGC AAGTCGGAAA 
GATATACCCT GTATATTTAG GGTCACAGCT TCCCAGCTCT CAGCATCTAA TAACAAATGT 
TCAATCCTGA TGCTAGCAGA CACTGAGAAT GAGAAGAATA AGTGGGTGGG AGTGCTGAGT 
GAATTGCACA AGATTTTGAA GAAAAACAAA TTCAGAGACC GCTCAGTCTA TGTTCCCAAA 
GAGGCTTATG ACAGCACTCT ACCCCTCATT AAAACAACCC AGGCAGCCGC AATCATAGAT 
CATGAAAGAA TTGCTTTGGG AAACGAAGAA GGGTTATTTG TTGTACATGT CACCAAAGAT 
GAAATTATTA GAGTTGGTGA CAATAAGAAG ATTCATCAGA TTGAACTCAT TCCAAATGAT 
CAGCTTGTTG CTGTGATCTC AGGACGAAAT CGTCATGTAC GACTTTTTCC TATGTCAGCA 
TTGGATGGGC GAGAGACCGA TTTTTACAAG CTGTCAGAAA CTAAAGGGTG TCAAACCGTA 
ACTTCTGGAA AGGTGCGCCA TGGAGCTCTC ACATGCCTGT GTGTGGCTAT GAAAAGGCAG 
GTCCTCTGTT ATGAACTATT TCAGAGCAAG ACCCGTCACA GAAAATTTAA AGAAATTCAA 
GTCCCATATA ATGTCCAGTG GATGGCAATC TTCAGTGAAC AACTCTGTGT GGGATTCCAG 
TCAGGATTTC TAAGATACCC CTTGAATGGA GAAGGAAATC CATACAGTAT GCTCCATTCA 
AATGACCATA CACTATCATT TATTGCACAT CAACCAATGG ATGCTATCTG CGCAGTTGAG 
ATCTCCAGTA AAGAATATCT GCTGTGTTTT AACAGCATTG GGATATACAC TGACTGCCAG 
GGCCGAAGAT CTAGACAACA GGAATTGATG TGGCCAGCAA ATCCTTCCTC TTGTTGTTAC 
AATGCACCAT ATCTCTCGGT GTACAGTGAA AATGCAGTTG ATATCTTTGA TGTGAACTCC 
ATGGAATGGA TTCAGACTCT TCCTCTCAAA AAGGTTCGAC CCTTAAACAA TGAAGGATCA 
TTAAATCTTT TAGGGTTGGA GACCATTAGA TTAATATATT TCAAAAATAA GATGGCAGAA 
GGGGACGAAC TGGTAGTACC TGAAACATCA GATAATAGTC GGAAACAAAT GGTTAGAAAC 
ATTAACAATA AGCGGCGTTA TTCCTTCAGA GTCCCAGAAG AGGAAAGGAT GCAGCAGAGG 
AGGGAAATGC TACGAGATCC AGAAATGAGA AATAAATTAA TTTCTAATCC AACTAATTTT 
AATCACATAG CACACATGGG TCCTGGAGAT GGAATACAGA TCCTGAAAGA TCTGCCCATG 
CCAGGTTTCC CTTATCCATC CCCTCATCAT CACTCCGGTC TGATCTCCTC TCCGATCAAC 
TTTGAACACA TCTATCACAT GACTGTTAAT TCTGCTGAAA AATTTCTCTC TCCTGATTCC 
ATAAACCCTG AATATTCCCC GTCTCTGCGC TCTGTCCCTG GTACACCAAG CTTTATGACT 
CTGAGGAACC CTCGGCCTCA GGAAAGTCGG ACAGTATTCA GTGGCTCAGT CAGTATTCCA 
TCTATCACCA AATCCCGCCC TGAGCCAGGC CGCTCCATGA GTGCTAGCAG TGGCTTGTCA 
GCAAGGTCAT CCGCACAGAA TGGCAGCGCA TTAAAGAGGG AATTCTCTGG AGGAAGCTAC 
AGTGCCAAGC GGCAGCCCAT GCCCTCCCCG TCAGAGGGCT CTTTGTCCTC TGGAGGCATG 
GACCAAGGAA GTGATGCCCC AGCGAGGGAC TTTGACGGAG AGGACTCTGA CTCTCCGAGG 
CATTCCACAG CTTCCAACAG TTCCAACCTA AGCAGCCCCC CAAGCCCAGC TTCACCCCGA 
AAAACCAAGA GCCTCTCCCT GGAGAGCACT GACCGCGGGA GCTGGGACCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_005273320.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005273377.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X7, mRNA.

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CloneID	OHu35141
Clone ID Related Accession (Same CDS sequence)	XM_005273320.3 , XM_005273320.4
Accession Version	XM_005273320.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005273320.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGTAG ACTCCACTCC ACTTTCAGTT CACACACCAA CCTTAAGGAA AAAAGGATGT 
CCTGGTTCAA CTGGCTTTCC ACCTAAGCGC AAGACTCACC AGTTTTTTGT AAAATCTTTT 
ACTACTCCTA CCAAGTGTCA TCAGTGTACC TCCTTGATGG TGGGTTTAAT AAGACAGGGC 
TGTTCATGTG AAGTGTGTGG ATTCTCATGC CATATAACTT GTGTAAACAA AGCTCCAACC 
ACTTGTCCAG TTCCTCCTGA ACAGACAAAA GGTCCCCTGG GTATAGATCC TCAGAAAGGA 
ATAGGAACAG CATATGAAGG TCATGTCAGG ATTCCTAAGC CAGCTGGAGT GAAGAAAGGG 
TGGCAGAGAG CACTGGCTAT AGTGTGTGAC TTCAAACTCT TTCTGTACGA TATTGCTGAA 
GGAAAAGCAT CTCAGCCCAG TGTTGTCATT AGTCAAGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT GTTATCCATG CAAGTCGGAA AGATATACCC 
TGTATATTTA GGGTCACAGC TTCCCAGCTC TCAGCATCTA ATAACAAATG TTCAATCCTG 
ATGCTAGCAG ACACTGAGAA TGAGAAGAAT AAGTGGGTGG GAGTGCTGAG TGAATTGCAC 
AAGATTTTGA AGAAAAACAA ATTCAGAGAC CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT 
GACAGCACTC TACCCCTCAT TAAAACAACC CAGGCAGCCG CAATCATAGA TCATGAAAGA 
ATTGCTTTGG GAAACGAAGA AGGGTTATTT GTTGTACATG TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ACAATAAGAA GATTCATCAG ATTGAACTCA TTCCAAATGA TCAGCTTGTT 
GCTGTGATCT CAGGACGAAA TCGTCATGTA CGACTTTTTC CTATGTCAGC ATTGGATGGG 
CGAGAGACCG ATTTTTACAA GCTGTCAGAA ACTAAAGGGT GTCAAACCGT AACTTCTGGA 
AAGGTGCGCC ATGGAGCTCT CACATGCCTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACCCGTCAC AGAAAATTTA AAGAAATTCA AGTCCCATAT 
AATGTCCAGT GGATGGCAAT CTTCAGTGAA CAACTCTGTG TGGGATTCCA GTCAGGATTT 
CTAAGATACC CCTTGAATGG AGAAGGAAAT CCATACAGTA TGCTCCATTC AAATGACCAT 
ACACTATCAT TTATTGCACA TCAACCAATG GATGCTATCT GCGCAGTTGA GATCTCCAGT 
AAAGAATATC TGCTGTGTTT TAACAGCATT GGGATATACA CTGACTGCCA GGGCCGAAGA 
TCTAGACAAC AGGAATTGAT GTGGCCAGCA AATCCTTCCT CTTGTTGTTA CAATGCACCA 
TATCTCTCGG TGTACAGTGA AAATGCAGTT GATATCTTTG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TTCCTCTCAA AAAGGTTCGA CCCTTAAACA ATGAAGGATC ATTAAATCTT 
TTAGGGTTGG AGACCATTAG ATTAATATAT TTCAAAAATA AGATGGCAGA AGGGGACGAA 
CTGGTAGTAC CTGAAACATC AGATAATAGT CGGAAACAAA TGGTTAGAAA CATTAACAAT 
AAGCGGCGTT ATTCCTTCAG AGTCCCAGAA GAGGAAAGGA TGCAGCAGAG GAGGGAAATG 
CTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAATC CAACTAATTT TAATCACATA 
GCACACATGG GTCCTGGAGA TGGAATACAG ATCCTGAAAG ATCTGCCCAT GCCAGGTTTC 
CCTTATCCAT CCCCTCATCA TCACTCCGGT CTGATCTCCT CTCCGATCAA CTTTGAACAC 
ATCTATCACA TGACTGTTAA TTCTGCTGAA AAATTTCTCT CTCCTGATTC CATAAACCCT 
GAATATTCCC CGTCTCTGCG CTCTGTCCCT GGTACACCAA GCTTTATGAC TCTGAGGAAC 
CCTCGGCCTC AGGAAAGTCG GACAGTATTC AGTGGCTCAG TCAGTATTCC ATCTATCACC 
AAATCCCGCC CTGAGCCAGG CCGCTCCATG AGTGCTAGCA GTGGCTTGTC AGCAAGGTCA 
TCCGCACAGA ATGGCAGCGC ATTAAAGAGG GAATTCTCTG GAGGAAGCTA CAGTGCCAAG 
CGGCAGCCCA TGCCCTCCCC GTCAGAGGGC TCTTTGTCCT CTGGAGGCAT GGACCAAGGA 
AGTGATGCCC CAGCGAGGGA CTTTGACGGA GAGGACTCTG ACTCTCCGAG GCATTCCACA 
GCTTCCAACA GTTCCAACCT AAGCAGCCCC CCAAGCCCAG CTTCACCCCG AAAAACCAAG 
AGCCTCTCCC TGGAGAGCAC TGACCGCGGG AGCTGGGACC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005273377.1
CDS	1152..6536
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_005273320.3
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005273320.3

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGTAG ACTCCACTCC ACTTTCAGTT CACACACCAA CCTTAAGGAA AAAAGGATGT 
CCTGGTTCAA CTGGCTTTCC ACCTAAGCGC AAGACTCACC AGTTTTTTGT AAAATCTTTT 
ACTACTCCTA CCAAGTGTCA TCAGTGTACC TCCTTGATGG TGGGTTTAAT AAGACAGGGC 
TGTTCATGTG AAGTGTGTGG ATTCTCATGC CATATAACTT GTGTAAACAA AGCTCCAACC 
ACTTGTCCAG TTCCTCCTGA ACAGACAAAA GGTCCCCTGG GTATAGATCC TCAGAAAGGA 
ATAGGAACAG CATATGAAGG TCATGTCAGG ATTCCTAAGC CAGCTGGAGT GAAGAAAGGG 
TGGCAGAGAG CACTGGCTAT AGTGTGTGAC TTCAAACTCT TTCTGTACGA TATTGCTGAA 
GGAAAAGCAT CTCAGCCCAG TGTTGTCATT AGTCAAGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT GTTATCCATG CAAGTCGGAA AGATATACCC 
TGTATATTTA GGGTCACAGC TTCCCAGCTC TCAGCATCTA ATAACAAATG TTCAATCCTG 
ATGCTAGCAG ACACTGAGAA TGAGAAGAAT AAGTGGGTGG GAGTGCTGAG TGAATTGCAC 
AAGATTTTGA AGAAAAACAA ATTCAGAGAC CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT 
GACAGCACTC TACCCCTCAT TAAAACAACC CAGGCAGCCG CAATCATAGA TCATGAAAGA 
ATTGCTTTGG GAAACGAAGA AGGGTTATTT GTTGTACATG TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ACAATAAGAA GATTCATCAG ATTGAACTCA TTCCAAATGA TCAGCTTGTT 
GCTGTGATCT CAGGACGAAA TCGTCATGTA CGACTTTTTC CTATGTCAGC ATTGGATGGG 
CGAGAGACCG ATTTTTACAA GCTGTCAGAA ACTAAAGGGT GTCAAACCGT AACTTCTGGA 
AAGGTGCGCC ATGGAGCTCT CACATGCCTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACCCGTCAC AGAAAATTTA AAGAAATTCA AGTCCCATAT 
AATGTCCAGT GGATGGCAAT CTTCAGTGAA CAACTCTGTG TGGGATTCCA GTCAGGATTT 
CTAAGATACC CCTTGAATGG AGAAGGAAAT CCATACAGTA TGCTCCATTC AAATGACCAT 
ACACTATCAT TTATTGCACA TCAACCAATG GATGCTATCT GCGCAGTTGA GATCTCCAGT 
AAAGAATATC TGCTGTGTTT TAACAGCATT GGGATATACA CTGACTGCCA GGGCCGAAGA 
TCTAGACAAC AGGAATTGAT GTGGCCAGCA AATCCTTCCT CTTGTTGTTA CAATGCACCA 
TATCTCTCGG TGTACAGTGA AAATGCAGTT GATATCTTTG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TTCCTCTCAA AAAGGTTCGA CCCTTAAACA ATGAAGGATC ATTAAATCTT 
TTAGGGTTGG AGACCATTAG ATTAATATAT TTCAAAAATA AGATGGCAGA AGGGGACGAA 
CTGGTAGTAC CTGAAACATC AGATAATAGT CGGAAACAAA TGGTTAGAAA CATTAACAAT 
AAGCGGCGTT ATTCCTTCAG AGTCCCAGAA GAGGAAAGGA TGCAGCAGAG GAGGGAAATG 
CTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAATC CAACTAATTT TAATCACATA 
GCACACATGG GTCCTGGAGA TGGAATACAG ATCCTGAAAG ATCTGCCCAT GCCAGGTTTC 
CCTTATCCAT CCCCTCATCA TCACTCCGGT CTGATCTCCT CTCCGATCAA CTTTGAACAC 
ATCTATCACA TGACTGTTAA TTCTGCTGAA AAATTTCTCT CTCCTGATTC CATAAACCCT 
GAATATTCCC CGTCTCTGCG CTCTGTCCCT GGTACACCAA GCTTTATGAC TCTGAGGAAC 
CCTCGGCCTC AGGAAAGTCG GACAGTATTC AGTGGCTCAG TCAGTATTCC ATCTATCACC 
AAATCCCGCC CTGAGCCAGG CCGCTCCATG AGTGCTAGCA GTGGCTTGTC AGCAAGGTCA 
TCCGCACAGA ATGGCAGCGC ATTAAAGAGG GAATTCTCTG GAGGAAGCTA CAGTGCCAAG 
CGGCAGCCCA TGCCCTCCCC GTCAGAGGGC TCTTTGTCCT CTGGAGGCAT GGACCAAGGA 
AGTGATGCCC CAGCGAGGGA CTTTGACGGA GAGGACTCTG ACTCTCCGAG GCATTCCACA 
GCTTCCAACA GTTCCAACCT AAGCAGCCCC CCAAGCCCAG CTTCACCCCG AAAAACCAAG 
AGCCTCTCCC TGGAGAGCAC TGACCGCGGG AGCTGGGACC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_005273320.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005273377.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X7, mRNA.

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CloneID	OHu35141
Clone ID Related Accession (Same CDS sequence)	XM_005273320.3 , XM_005273320.4
Accession Version	XM_005273320.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005273320.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGTAG ACTCCACTCC ACTTTCAGTT CACACACCAA CCTTAAGGAA AAAAGGATGT 
CCTGGTTCAA CTGGCTTTCC ACCTAAGCGC AAGACTCACC AGTTTTTTGT AAAATCTTTT 
ACTACTCCTA CCAAGTGTCA TCAGTGTACC TCCTTGATGG TGGGTTTAAT AAGACAGGGC 
TGTTCATGTG AAGTGTGTGG ATTCTCATGC CATATAACTT GTGTAAACAA AGCTCCAACC 
ACTTGTCCAG TTCCTCCTGA ACAGACAAAA GGTCCCCTGG GTATAGATCC TCAGAAAGGA 
ATAGGAACAG CATATGAAGG TCATGTCAGG ATTCCTAAGC CAGCTGGAGT GAAGAAAGGG 
TGGCAGAGAG CACTGGCTAT AGTGTGTGAC TTCAAACTCT TTCTGTACGA TATTGCTGAA 
GGAAAAGCAT CTCAGCCCAG TGTTGTCATT AGTCAAGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT GTTATCCATG CAAGTCGGAA AGATATACCC 
TGTATATTTA GGGTCACAGC TTCCCAGCTC TCAGCATCTA ATAACAAATG TTCAATCCTG 
ATGCTAGCAG ACACTGAGAA TGAGAAGAAT AAGTGGGTGG GAGTGCTGAG TGAATTGCAC 
AAGATTTTGA AGAAAAACAA ATTCAGAGAC CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT 
GACAGCACTC TACCCCTCAT TAAAACAACC CAGGCAGCCG CAATCATAGA TCATGAAAGA 
ATTGCTTTGG GAAACGAAGA AGGGTTATTT GTTGTACATG TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ACAATAAGAA GATTCATCAG ATTGAACTCA TTCCAAATGA TCAGCTTGTT 
GCTGTGATCT CAGGACGAAA TCGTCATGTA CGACTTTTTC CTATGTCAGC ATTGGATGGG 
CGAGAGACCG ATTTTTACAA GCTGTCAGAA ACTAAAGGGT GTCAAACCGT AACTTCTGGA 
AAGGTGCGCC ATGGAGCTCT CACATGCCTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACCCGTCAC AGAAAATTTA AAGAAATTCA AGTCCCATAT 
AATGTCCAGT GGATGGCAAT CTTCAGTGAA CAACTCTGTG TGGGATTCCA GTCAGGATTT 
CTAAGATACC CCTTGAATGG AGAAGGAAAT CCATACAGTA TGCTCCATTC AAATGACCAT 
ACACTATCAT TTATTGCACA TCAACCAATG GATGCTATCT GCGCAGTTGA GATCTCCAGT 
AAAGAATATC TGCTGTGTTT TAACAGCATT GGGATATACA CTGACTGCCA GGGCCGAAGA 
TCTAGACAAC AGGAATTGAT GTGGCCAGCA AATCCTTCCT CTTGTTGTTA CAATGCACCA 
TATCTCTCGG TGTACAGTGA AAATGCAGTT GATATCTTTG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TTCCTCTCAA AAAGGTTCGA CCCTTAAACA ATGAAGGATC ATTAAATCTT 
TTAGGGTTGG AGACCATTAG ATTAATATAT TTCAAAAATA AGATGGCAGA AGGGGACGAA 
CTGGTAGTAC CTGAAACATC AGATAATAGT CGGAAACAAA TGGTTAGAAA CATTAACAAT 
AAGCGGCGTT ATTCCTTCAG AGTCCCAGAA GAGGAAAGGA TGCAGCAGAG GAGGGAAATG 
CTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAATC CAACTAATTT TAATCACATA 
GCACACATGG GTCCTGGAGA TGGAATACAG ATCCTGAAAG ATCTGCCCAT GCCAGGTTTC 
CCTTATCCAT CCCCTCATCA TCACTCCGGT CTGATCTCCT CTCCGATCAA CTTTGAACAC 
ATCTATCACA TGACTGTTAA TTCTGCTGAA AAATTTCTCT CTCCTGATTC CATAAACCCT 
GAATATTCCC CGTCTCTGCG CTCTGTCCCT GGTACACCAA GCTTTATGAC TCTGAGGAAC 
CCTCGGCCTC AGGAAAGTCG GACAGTATTC AGTGGCTCAG TCAGTATTCC ATCTATCACC 
AAATCCCGCC CTGAGCCAGG CCGCTCCATG AGTGCTAGCA GTGGCTTGTC AGCAAGGTCA 
TCCGCACAGA ATGGCAGCGC ATTAAAGAGG GAATTCTCTG GAGGAAGCTA CAGTGCCAAG 
CGGCAGCCCA TGCCCTCCCC GTCAGAGGGC TCTTTGTCCT CTGGAGGCAT GGACCAAGGA 
AGTGATGCCC CAGCGAGGGA CTTTGACGGA GAGGACTCTG ACTCTCCGAG GCATTCCACA 
GCTTCCAACA GTTCCAACCT AAGCAGCCCC CCAAGCCCAG CTTCACCCCG AAAAACCAAG 
AGCCTCTCCC TGGAGAGCAC TGACCGCGGG AGCTGGGACC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005273377.1
CDS	1152..6536
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_005273320.4
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005273320.4

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGTAG ACTCCACTCC ACTTTCAGTT CACACACCAA CCTTAAGGAA AAAAGGATGT 
CCTGGTTCAA CTGGCTTTCC ACCTAAGCGC AAGACTCACC AGTTTTTTGT AAAATCTTTT 
ACTACTCCTA CCAAGTGTCA TCAGTGTACC TCCTTGATGG TGGGTTTAAT AAGACAGGGC 
TGTTCATGTG AAGTGTGTGG ATTCTCATGC CATATAACTT GTGTAAACAA AGCTCCAACC 
ACTTGTCCAG TTCCTCCTGA ACAGACAAAA GGTCCCCTGG GTATAGATCC TCAGAAAGGA 
ATAGGAACAG CATATGAAGG TCATGTCAGG ATTCCTAAGC CAGCTGGAGT GAAGAAAGGG 
TGGCAGAGAG CACTGGCTAT AGTGTGTGAC TTCAAACTCT TTCTGTACGA TATTGCTGAA 
GGAAAAGCAT CTCAGCCCAG TGTTGTCATT AGTCAAGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT GTTATCCATG CAAGTCGGAA AGATATACCC 
TGTATATTTA GGGTCACAGC TTCCCAGCTC TCAGCATCTA ATAACAAATG TTCAATCCTG 
ATGCTAGCAG ACACTGAGAA TGAGAAGAAT AAGTGGGTGG GAGTGCTGAG TGAATTGCAC 
AAGATTTTGA AGAAAAACAA ATTCAGAGAC CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT 
GACAGCACTC TACCCCTCAT TAAAACAACC CAGGCAGCCG CAATCATAGA TCATGAAAGA 
ATTGCTTTGG GAAACGAAGA AGGGTTATTT GTTGTACATG TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ACAATAAGAA GATTCATCAG ATTGAACTCA TTCCAAATGA TCAGCTTGTT 
GCTGTGATCT CAGGACGAAA TCGTCATGTA CGACTTTTTC CTATGTCAGC ATTGGATGGG 
CGAGAGACCG ATTTTTACAA GCTGTCAGAA ACTAAAGGGT GTCAAACCGT AACTTCTGGA 
AAGGTGCGCC ATGGAGCTCT CACATGCCTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACCCGTCAC AGAAAATTTA AAGAAATTCA AGTCCCATAT 
AATGTCCAGT GGATGGCAAT CTTCAGTGAA CAACTCTGTG TGGGATTCCA GTCAGGATTT 
CTAAGATACC CCTTGAATGG AGAAGGAAAT CCATACAGTA TGCTCCATTC AAATGACCAT 
ACACTATCAT TTATTGCACA TCAACCAATG GATGCTATCT GCGCAGTTGA GATCTCCAGT 
AAAGAATATC TGCTGTGTTT TAACAGCATT GGGATATACA CTGACTGCCA GGGCCGAAGA 
TCTAGACAAC AGGAATTGAT GTGGCCAGCA AATCCTTCCT CTTGTTGTTA CAATGCACCA 
TATCTCTCGG TGTACAGTGA AAATGCAGTT GATATCTTTG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TTCCTCTCAA AAAGGTTCGA CCCTTAAACA ATGAAGGATC ATTAAATCTT 
TTAGGGTTGG AGACCATTAG ATTAATATAT TTCAAAAATA AGATGGCAGA AGGGGACGAA 
CTGGTAGTAC CTGAAACATC AGATAATAGT CGGAAACAAA TGGTTAGAAA CATTAACAAT 
AAGCGGCGTT ATTCCTTCAG AGTCCCAGAA GAGGAAAGGA TGCAGCAGAG GAGGGAAATG 
CTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAATC CAACTAATTT TAATCACATA 
GCACACATGG GTCCTGGAGA TGGAATACAG ATCCTGAAAG ATCTGCCCAT GCCAGGTTTC 
CCTTATCCAT CCCCTCATCA TCACTCCGGT CTGATCTCCT CTCCGATCAA CTTTGAACAC 
ATCTATCACA TGACTGTTAA TTCTGCTGAA AAATTTCTCT CTCCTGATTC CATAAACCCT 
GAATATTCCC CGTCTCTGCG CTCTGTCCCT GGTACACCAA GCTTTATGAC TCTGAGGAAC 
CCTCGGCCTC AGGAAAGTCG GACAGTATTC AGTGGCTCAG TCAGTATTCC ATCTATCACC 
AAATCCCGCC CTGAGCCAGG CCGCTCCATG AGTGCTAGCA GTGGCTTGTC AGCAAGGTCA 
TCCGCACAGA ATGGCAGCGC ATTAAAGAGG GAATTCTCTG GAGGAAGCTA CAGTGCCAAG 
CGGCAGCCCA TGCCCTCCCC GTCAGAGGGC TCTTTGTCCT CTGGAGGCAT GGACCAAGGA 
AGTGATGCCC CAGCGAGGGA CTTTGACGGA GAGGACTCTG ACTCTCCGAG GCATTCCACA 
GCTTCCAACA GTTCCAACCT AAGCAGCCCC CCAAGCCCAG CTTCACCCCG AAAAACCAAG 
AGCCTCTCCC TGGAGAGCAC TGACCGCGGG AGCTGGGACC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_005273321.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005273378.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X9, mRNA.

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CloneID	OHu35142
Clone ID Related Accession (Same CDS sequence)	XM_005273321.3 , XM_005273321.4
Accession Version	XM_005273321.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005273321.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCCACTC CACTTTCAGT TCACACACCA 
ACCTTAAGGA AAAAAGGATG TCCTGGTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAC 
CAGTTTTTTG TAAAATCTTT TACTACTCCT ACCAAGTGTC ATCAGTGTAC CTCCTTGATG 
GTGGGTTTAA TAAGACAGGG CTGTTCATGT GAAGTGTGTG GATTCTCATG CCATATAACT 
TGTGTAAACA AAGCTCCAAC CACTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCCCTG 
GGTATAGATC CTCAGAAAGG AATAGGAACA GCATATGAAG GTCATGTCAG GATTCCTAAG 
CCAGCTGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTA TAGTGTGTGA CTTCAAACTC 
TTTCTGTACG ATATTGCTGA AGGAAAAGCA TCTCAGCCCA GTGTTGTCAT TAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATCCAT 
GCAAGTCGGA AAGATATACC CTGTATATTT AGGGTCACAG CTTCCCAGCT CTCAGCATCT 
AATAACAAAT GTTCAATCCT GATGCTAGCA GACACTGAGA ATGAGAAGAA TAAGTGGGTG 
GGAGTGCTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTACCCCTCA TTAAAACAAC CCAGGCAGCC 
GCAATCATAG ATCATGAAAG AATTGCTTTG GGAAACGAAG AAGGGTTATT TGTTGTACAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATGTCAG CATTGGATGG GCGAGAGACC GATTTTTACA AGCTGTCAGA AACTAAAGGG 
TGTCAAACCG TAACTTCTGG AAAGGTGCGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGAAAATTT 
AAAGAAATTC AAGTCCCATA TAATGTCCAG TGGATGGCAA TCTTCAGTGA ACAACTCTGT 
GTGGGATTCC AGTCAGGATT TCTAAGATAC CCCTTGAATG GAGAAGGAAA TCCATACAGT 
ATGCTCCATT CAAATGACCA TACACTATCA TTTATTGCAC ATCAACCAAT GGATGCTATC 
TGCGCAGTTG AGATCTCCAG TAAAGAATAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAATTGA TGTGGCCAGC AAATCCTTCC 
TCTTGTTGTT ACAATGCACC ATATCTCTCG GTGTACAGTG AAAATGCAGT TGATATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTTCCTCTCA AAAAGGTTCG ACCCTTAAAC 
AATGAAGGAT CATTAAATCT TTTAGGGTTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGACGA ACTGGTAGTA CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ACATTAACAA TAAGCGGCGT TATTCCTTCA GAGTCCCAGA AGAGGAAAGG 
ATGCAGCAGA GGAGGGAAAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAT 
CCAACTAATT TTAATCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTGAAA 
GATCTGCCCA TGCCAGGTTT CCCTTATCCA TCCCCTCATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAACA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGAATATTCC CCGTCTCTGC GCTCTGTCCC TGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGTC GGACAGTATT CAGTGGCTCA 
GTCAGTATTC CATCTATCAC CAAATCCCGC CCTGAGCCAG GCCGCTCCAT GAGTGCTAGC 
AGTGGCTTGT CAGCAAGGTC ATCCGCACAG AATGGCAGCG CATTAAAGAG GGAATTCTCT 
GGAGGAAGCT ACAGTGCCAA GCGGCAGCCC ATGCCCTCCC CGTCAGAGGG CTCTTTGTCC 
TCTGGAGGCA TGGACCAAGG AAGTGATGCC CCAGCGAGGG ACTTTGACGG AGAGGACTCT 
GACTCTCCGA GGCATTCCAC AGCTTCCAAC AGTTCCAACC TAAGCAGCCC CCCAAGCCCA 
GCTTCACCCC GAAAAACCAA GAGCCTCTCC CTGGAGAGCA CTGACCGCGG GAGCTGGGAC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005273378.1
CDS	1056..6401
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_005273321.3
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005273321.3

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCCACTC CACTTTCAGT TCACACACCA 
ACCTTAAGGA AAAAAGGATG TCCTGGTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAC 
CAGTTTTTTG TAAAATCTTT TACTACTCCT ACCAAGTGTC ATCAGTGTAC CTCCTTGATG 
GTGGGTTTAA TAAGACAGGG CTGTTCATGT GAAGTGTGTG GATTCTCATG CCATATAACT 
TGTGTAAACA AAGCTCCAAC CACTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCCCTG 
GGTATAGATC CTCAGAAAGG AATAGGAACA GCATATGAAG GTCATGTCAG GATTCCTAAG 
CCAGCTGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTA TAGTGTGTGA CTTCAAACTC 
TTTCTGTACG ATATTGCTGA AGGAAAAGCA TCTCAGCCCA GTGTTGTCAT TAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATCCAT 
GCAAGTCGGA AAGATATACC CTGTATATTT AGGGTCACAG CTTCCCAGCT CTCAGCATCT 
AATAACAAAT GTTCAATCCT GATGCTAGCA GACACTGAGA ATGAGAAGAA TAAGTGGGTG 
GGAGTGCTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTACCCCTCA TTAAAACAAC CCAGGCAGCC 
GCAATCATAG ATCATGAAAG AATTGCTTTG GGAAACGAAG AAGGGTTATT TGTTGTACAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATGTCAG CATTGGATGG GCGAGAGACC GATTTTTACA AGCTGTCAGA AACTAAAGGG 
TGTCAAACCG TAACTTCTGG AAAGGTGCGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGAAAATTT 
AAAGAAATTC AAGTCCCATA TAATGTCCAG TGGATGGCAA TCTTCAGTGA ACAACTCTGT 
GTGGGATTCC AGTCAGGATT TCTAAGATAC CCCTTGAATG GAGAAGGAAA TCCATACAGT 
ATGCTCCATT CAAATGACCA TACACTATCA TTTATTGCAC ATCAACCAAT GGATGCTATC 
TGCGCAGTTG AGATCTCCAG TAAAGAATAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAATTGA TGTGGCCAGC AAATCCTTCC 
TCTTGTTGTT ACAATGCACC ATATCTCTCG GTGTACAGTG AAAATGCAGT TGATATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTTCCTCTCA AAAAGGTTCG ACCCTTAAAC 
AATGAAGGAT CATTAAATCT TTTAGGGTTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGACGA ACTGGTAGTA CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ACATTAACAA TAAGCGGCGT TATTCCTTCA GAGTCCCAGA AGAGGAAAGG 
ATGCAGCAGA GGAGGGAAAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAT 
CCAACTAATT TTAATCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTGAAA 
GATCTGCCCA TGCCAGGTTT CCCTTATCCA TCCCCTCATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAACA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGAATATTCC CCGTCTCTGC GCTCTGTCCC TGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGTC GGACAGTATT CAGTGGCTCA 
GTCAGTATTC CATCTATCAC CAAATCCCGC CCTGAGCCAG GCCGCTCCAT GAGTGCTAGC 
AGTGGCTTGT CAGCAAGGTC ATCCGCACAG AATGGCAGCG CATTAAAGAG GGAATTCTCT 
GGAGGAAGCT ACAGTGCCAA GCGGCAGCCC ATGCCCTCCC CGTCAGAGGG CTCTTTGTCC 
TCTGGAGGCA TGGACCAAGG AAGTGATGCC CCAGCGAGGG ACTTTGACGG AGAGGACTCT 
GACTCTCCGA GGCATTCCAC AGCTTCCAAC AGTTCCAACC TAAGCAGCCC CCCAAGCCCA 
GCTTCACCCC GAAAAACCAA GAGCCTCTCC CTGGAGAGCA CTGACCGCGG GAGCTGGGAC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_005273321.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005273378.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X9, mRNA.

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CloneID	OHu35142
Clone ID Related Accession (Same CDS sequence)	XM_005273321.3 , XM_005273321.4
Accession Version	XM_005273321.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005273321.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCCACTC CACTTTCAGT TCACACACCA 
ACCTTAAGGA AAAAAGGATG TCCTGGTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAC 
CAGTTTTTTG TAAAATCTTT TACTACTCCT ACCAAGTGTC ATCAGTGTAC CTCCTTGATG 
GTGGGTTTAA TAAGACAGGG CTGTTCATGT GAAGTGTGTG GATTCTCATG CCATATAACT 
TGTGTAAACA AAGCTCCAAC CACTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCCCTG 
GGTATAGATC CTCAGAAAGG AATAGGAACA GCATATGAAG GTCATGTCAG GATTCCTAAG 
CCAGCTGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTA TAGTGTGTGA CTTCAAACTC 
TTTCTGTACG ATATTGCTGA AGGAAAAGCA TCTCAGCCCA GTGTTGTCAT TAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATCCAT 
GCAAGTCGGA AAGATATACC CTGTATATTT AGGGTCACAG CTTCCCAGCT CTCAGCATCT 
AATAACAAAT GTTCAATCCT GATGCTAGCA GACACTGAGA ATGAGAAGAA TAAGTGGGTG 
GGAGTGCTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTACCCCTCA TTAAAACAAC CCAGGCAGCC 
GCAATCATAG ATCATGAAAG AATTGCTTTG GGAAACGAAG AAGGGTTATT TGTTGTACAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATGTCAG CATTGGATGG GCGAGAGACC GATTTTTACA AGCTGTCAGA AACTAAAGGG 
TGTCAAACCG TAACTTCTGG AAAGGTGCGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGAAAATTT 
AAAGAAATTC AAGTCCCATA TAATGTCCAG TGGATGGCAA TCTTCAGTGA ACAACTCTGT 
GTGGGATTCC AGTCAGGATT TCTAAGATAC CCCTTGAATG GAGAAGGAAA TCCATACAGT 
ATGCTCCATT CAAATGACCA TACACTATCA TTTATTGCAC ATCAACCAAT GGATGCTATC 
TGCGCAGTTG AGATCTCCAG TAAAGAATAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAATTGA TGTGGCCAGC AAATCCTTCC 
TCTTGTTGTT ACAATGCACC ATATCTCTCG GTGTACAGTG AAAATGCAGT TGATATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTTCCTCTCA AAAAGGTTCG ACCCTTAAAC 
AATGAAGGAT CATTAAATCT TTTAGGGTTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGACGA ACTGGTAGTA CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ACATTAACAA TAAGCGGCGT TATTCCTTCA GAGTCCCAGA AGAGGAAAGG 
ATGCAGCAGA GGAGGGAAAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAT 
CCAACTAATT TTAATCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTGAAA 
GATCTGCCCA TGCCAGGTTT CCCTTATCCA TCCCCTCATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAACA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGAATATTCC CCGTCTCTGC GCTCTGTCCC TGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGTC GGACAGTATT CAGTGGCTCA 
GTCAGTATTC CATCTATCAC CAAATCCCGC CCTGAGCCAG GCCGCTCCAT GAGTGCTAGC 
AGTGGCTTGT CAGCAAGGTC ATCCGCACAG AATGGCAGCG CATTAAAGAG GGAATTCTCT 
GGAGGAAGCT ACAGTGCCAA GCGGCAGCCC ATGCCCTCCC CGTCAGAGGG CTCTTTGTCC 
TCTGGAGGCA TGGACCAAGG AAGTGATGCC CCAGCGAGGG ACTTTGACGG AGAGGACTCT 
GACTCTCCGA GGCATTCCAC AGCTTCCAAC AGTTCCAACC TAAGCAGCCC CCCAAGCCCA 
GCTTCACCCC GAAAAACCAA GAGCCTCTCC CTGGAGAGCA CTGACCGCGG GAGCTGGGAC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005273378.1
CDS	969..6314
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_005273321.4
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005273321.4

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCCACTC CACTTTCAGT TCACACACCA 
ACCTTAAGGA AAAAAGGATG TCCTGGTTCA ACTGGCTTTC CACCTAAGCG CAAGACTCAC 
CAGTTTTTTG TAAAATCTTT TACTACTCCT ACCAAGTGTC ATCAGTGTAC CTCCTTGATG 
GTGGGTTTAA TAAGACAGGG CTGTTCATGT GAAGTGTGTG GATTCTCATG CCATATAACT 
TGTGTAAACA AAGCTCCAAC CACTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCCCTG 
GGTATAGATC CTCAGAAAGG AATAGGAACA GCATATGAAG GTCATGTCAG GATTCCTAAG 
CCAGCTGGAG TGAAGAAAGG GTGGCAGAGA GCACTGGCTA TAGTGTGTGA CTTCAAACTC 
TTTCTGTACG ATATTGCTGA AGGAAAAGCA TCTCAGCCCA GTGTTGTCAT TAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATCCAT 
GCAAGTCGGA AAGATATACC CTGTATATTT AGGGTCACAG CTTCCCAGCT CTCAGCATCT 
AATAACAAAT GTTCAATCCT GATGCTAGCA GACACTGAGA ATGAGAAGAA TAAGTGGGTG 
GGAGTGCTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTACCCCTCA TTAAAACAAC CCAGGCAGCC 
GCAATCATAG ATCATGAAAG AATTGCTTTG GGAAACGAAG AAGGGTTATT TGTTGTACAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATGTCAG CATTGGATGG GCGAGAGACC GATTTTTACA AGCTGTCAGA AACTAAAGGG 
TGTCAAACCG TAACTTCTGG AAAGGTGCGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGAAAATTT 
AAAGAAATTC AAGTCCCATA TAATGTCCAG TGGATGGCAA TCTTCAGTGA ACAACTCTGT 
GTGGGATTCC AGTCAGGATT TCTAAGATAC CCCTTGAATG GAGAAGGAAA TCCATACAGT 
ATGCTCCATT CAAATGACCA TACACTATCA TTTATTGCAC ATCAACCAAT GGATGCTATC 
TGCGCAGTTG AGATCTCCAG TAAAGAATAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAATTGA TGTGGCCAGC AAATCCTTCC 
TCTTGTTGTT ACAATGCACC ATATCTCTCG GTGTACAGTG AAAATGCAGT TGATATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTTCCTCTCA AAAAGGTTCG ACCCTTAAAC 
AATGAAGGAT CATTAAATCT TTTAGGGTTG GAGACCATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGACGA ACTGGTAGTA CCTGAAACAT CAGATAATAG TCGGAAACAA 
ATGGTTAGAA ACATTAACAA TAAGCGGCGT TATTCCTTCA GAGTCCCAGA AGAGGAAAGG 
ATGCAGCAGA GGAGGGAAAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAT 
CCAACTAATT TTAATCACAT AGCACACATG GGTCCTGGAG ATGGAATACA GATCCTGAAA 
GATCTGCCCA TGCCAGGTTT CCCTTATCCA TCCCCTCATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAACA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGAATATTCC CCGTCTCTGC GCTCTGTCCC TGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGTC GGACAGTATT CAGTGGCTCA 
GTCAGTATTC CATCTATCAC CAAATCCCGC CCTGAGCCAG GCCGCTCCAT GAGTGCTAGC 
AGTGGCTTGT CAGCAAGGTC ATCCGCACAG AATGGCAGCG CATTAAAGAG GGAATTCTCT 
GGAGGAAGCT ACAGTGCCAA GCGGCAGCCC ATGCCCTCCC CGTCAGAGGG CTCTTTGTCC 
TCTGGAGGCA TGGACCAAGG AAGTGATGCC CCAGCGAGGG ACTTTGACGG AGAGGACTCT 
GACTCTCCGA GGCATTCCAC AGCTTCCAAC AGTTCCAACC TAAGCAGCCC CCCAAGCCCA 
GCTTCACCCC GAAAAACCAA GAGCCTCTCC CTGGAGAGCA CTGACCGCGG GAGCTGGGAC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_005273322.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005273379.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X12, mRNA.

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CloneID	OHu35144
Clone ID Related Accession (Same CDS sequence)	XM_005273322.4 , XM_005273322.3
Accession Version	XM_005273322.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005273322.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGAT CCCGTTGAGA ACACATATGT ATGGAACCCG 
AGCGTCAAGT TTCACATCCA GTCACGGTCC ACATCTCCTT CCACATCTAG TGAAGCTGAG 
CCAGTTAAGA CTGTAGACTC CACTCCACTT TCAGTTCACA CACCAACCTT AAGGAAAAAA 
GGATGTCCTG GTTCAACTGG CTTTCCACCT AAGCGCAAGA CTCACCAGTT TTTTGTAAAA 
TCTTTTACTA CTCCTACCAA GTGTCATCAG TGTACCTCCT TGATGGTGGG TTTAATAAGA 
CAGGGCTGTT CATGTGAAGT GTGTGGATTC TCATGCCATA TAACTTGTGT AAACAAAGCT 
CCAACCACTT GTCCAGTTCC TCCTGAACAG ACAAAAGGTC CCCTGGGTAT AGATCCTCAG 
AAAGGAATAG GAACAGCATA TGAAGGTCAT GTCAGGATTC CTAAGCCAGC TGGAGTGAAG 
AAAGGGTGGC AGAGAGCACT GGCTATAGTG TGTGACTTCA AACTCTTTCT GTACGATATT 
GCTGAAGGAA AAGCATCTCA GCCCAGTGTT GTCATTAGTC AAGTGATTGA CATGAGGGAT 
GAAGAATTTT CTGTGAGTTC AGTCTTGGCT TCTGATGTTA TCCATGCAAG TCGGAAAGAT 
ATACCCTGTA TATTTAGGGT CACAGCTTCC CAGCTCTCAG CATCTAATAA CAAATGTTCA 
ATCCTGATGC TAGCAGACAC TGAGAATGAG AAGAATAAGT GGGTGGGAGT GCTGAGTGAA 
TTGCACAAGA TTTTGAAGAA AAACAAATTC AGAGACCGCT CAGTCTATGT TCCCAAAGAG 
GCTTATGACA GCACTCTACC CCTCATTAAA ACAACCCAGG CAGCCGCAAT CATAGATCAT 
GAAAGAATTG CTTTGGGAAA CGAAGAAGGG TTATTTGTTG TACATGTCAC CAAAGATGAA 
ATTATTAGAG TTGGTGACAA TAAGAAGATT CATCAGATTG AACTCATTCC AAATGATCAG 
CTTGTTGCTG TGATCTCAGG ACGAAATCGT CATGTACGAC TTTTTCCTAT GTCAGCATTG 
GATGGGCGAG AGACCGATTT TTACAAGCTG TCAGAAACTA AAGGGTGTCA AACCGTAACT 
TCTGGAAAGG TGCGCCATGG AGCTCTCACA TGCCTGTGTG TGGCTATGAA AAGGCAGGTC 
CTCTGTTATG AACTATTTCA GAGCAAGACC CGTCACAGAA AATTTAAAGA AATTCAAGTC 
CCATATAATG TCCAGTGGAT GGCAATCTTC AGTGAACAAC TCTGTGTGGG ATTCCAGTCA 
GGATTTCTAA GATACCCCTT GAATGGAGAA GGAAATCCAT ACAGTATGCT CCATTCAAAT 
GACCATACAC TATCATTTAT TGCACATCAA CCAATGGATG CTATCTGCGC AGTTGAGATC 
TCCAGTAAAG AATATCTGCT GTGTTTTAAC AGCATTGGGA TATACACTGA CTGCCAGGGC 
CGAAGATCTA GACAACAGGA ATTGATGTGG CCAGCAAATC CTTCCTCTTG TTGTTACAAT 
GCACCATATC TCTCGGTGTA CAGTGAAAAT GCAGTTGATA TCTTTGATGT GAACTCCATG 
GAATGGATTC AGACTCTTCC TCTCAAAAAG GTTCGACCCT TAAACAATGA AGGATCATTA 
AATCTTTTAG GGTTGGAGAC CATTAGATTA ATATATTTCA AAAATAAGAT GGCAGAAGGG 
GACGAACTGG TAGTACCTGA AACATCAGAT AATAGTCGGA AACAAATGGT TAGAAACATT 
AACAATAAGC GGCGTTATTC CTTCAGAGTC CCAGAAGAGG AAAGGATGCA GCAGAGGAGG 
GAAATGCTAC GAGATCCAGA AATGAGAAAT AAATTAATTT CTAATCCAAC TAATTTTAAT 
CACATAGCAC ACATGGGTCC TGGAGATGGA ATACAGATCC TGAAAGATCT GCCCATGAAC 
CCTCGGCCTC AGGAAAGTCG GACAGTATTC AGTGGCTCAG TCAGTATTCC ATCTATCACC 
AAATCCCGCC CTGAGCCAGG CCGCTCCATG AGTGCTAGCA GTGGCTTGTC AGCAAGGTCA 
TCCGCACAGA ATGGCAGCGC ATTAAAGAGG GAATTCTCTG GAGGAAGCTA CAGTGCCAAG 
CGGCAGCCCA TGCCCTCCCC GTCAGAGGGC TCTTTGTCCT CTGGAGGCAT GGACCAAGGA 
AGTGATGCCC CAGCGAGGGA CTTTGACGGA GAGGACTCTG ACTCTCCGAG GCATTCCACA 
GCTTCCAACA GTTCCAACCT AAGCAGCCCC CCAAGCCCAG CTTCACCCCG AAAAACCAAG 
AGCCTCTCCC TGGAGAGCAC TGACCGCGGG AGCTGGGACC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005273379.1
CDS	969..6233
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETDPVENTYVWNPSVKFHIQSRSTSPSTSSEAEPVKTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_005273322.4
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005273322.4

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGAT CCCGTTGAGA ACACATATGT ATGGAACCCG 
AGCGTCAAGT TTCACATCCA GTCACGGTCC ACATCTCCTT CCACATCTAG TGAAGCTGAG 
CCAGTTAAGA CTGTAGACTC CACTCCACTT TCAGTTCACA CACCAACCTT AAGGAAAAAA 
GGATGTCCTG GTTCAACTGG CTTTCCACCT AAGCGCAAGA CTCACCAGTT TTTTGTAAAA 
TCTTTTACTA CTCCTACCAA GTGTCATCAG TGTACCTCCT TGATGGTGGG TTTAATAAGA 
CAGGGCTGTT CATGTGAAGT GTGTGGATTC TCATGCCATA TAACTTGTGT AAACAAAGCT 
CCAACCACTT GTCCAGTTCC TCCTGAACAG ACAAAAGGTC CCCTGGGTAT AGATCCTCAG 
AAAGGAATAG GAACAGCATA TGAAGGTCAT GTCAGGATTC CTAAGCCAGC TGGAGTGAAG 
AAAGGGTGGC AGAGAGCACT GGCTATAGTG TGTGACTTCA AACTCTTTCT GTACGATATT 
GCTGAAGGAA AAGCATCTCA GCCCAGTGTT GTCATTAGTC AAGTGATTGA CATGAGGGAT 
GAAGAATTTT CTGTGAGTTC AGTCTTGGCT TCTGATGTTA TCCATGCAAG TCGGAAAGAT 
ATACCCTGTA TATTTAGGGT CACAGCTTCC CAGCTCTCAG CATCTAATAA CAAATGTTCA 
ATCCTGATGC TAGCAGACAC TGAGAATGAG AAGAATAAGT GGGTGGGAGT GCTGAGTGAA 
TTGCACAAGA TTTTGAAGAA AAACAAATTC AGAGACCGCT CAGTCTATGT TCCCAAAGAG 
GCTTATGACA GCACTCTACC CCTCATTAAA ACAACCCAGG CAGCCGCAAT CATAGATCAT 
GAAAGAATTG CTTTGGGAAA CGAAGAAGGG TTATTTGTTG TACATGTCAC CAAAGATGAA 
ATTATTAGAG TTGGTGACAA TAAGAAGATT CATCAGATTG AACTCATTCC AAATGATCAG 
CTTGTTGCTG TGATCTCAGG ACGAAATCGT CATGTACGAC TTTTTCCTAT GTCAGCATTG 
GATGGGCGAG AGACCGATTT TTACAAGCTG TCAGAAACTA AAGGGTGTCA AACCGTAACT 
TCTGGAAAGG TGCGCCATGG AGCTCTCACA TGCCTGTGTG TGGCTATGAA AAGGCAGGTC 
CTCTGTTATG AACTATTTCA GAGCAAGACC CGTCACAGAA AATTTAAAGA AATTCAAGTC 
CCATATAATG TCCAGTGGAT GGCAATCTTC AGTGAACAAC TCTGTGTGGG ATTCCAGTCA 
GGATTTCTAA GATACCCCTT GAATGGAGAA GGAAATCCAT ACAGTATGCT CCATTCAAAT 
GACCATACAC TATCATTTAT TGCACATCAA CCAATGGATG CTATCTGCGC AGTTGAGATC 
TCCAGTAAAG AATATCTGCT GTGTTTTAAC AGCATTGGGA TATACACTGA CTGCCAGGGC 
CGAAGATCTA GACAACAGGA ATTGATGTGG CCAGCAAATC CTTCCTCTTG TTGTTACAAT 
GCACCATATC TCTCGGTGTA CAGTGAAAAT GCAGTTGATA TCTTTGATGT GAACTCCATG 
GAATGGATTC AGACTCTTCC TCTCAAAAAG GTTCGACCCT TAAACAATGA AGGATCATTA 
AATCTTTTAG GGTTGGAGAC CATTAGATTA ATATATTTCA AAAATAAGAT GGCAGAAGGG 
GACGAACTGG TAGTACCTGA AACATCAGAT AATAGTCGGA AACAAATGGT TAGAAACATT 
AACAATAAGC GGCGTTATTC CTTCAGAGTC CCAGAAGAGG AAAGGATGCA GCAGAGGAGG 
GAAATGCTAC GAGATCCAGA AATGAGAAAT AAATTAATTT CTAATCCAAC TAATTTTAAT 
CACATAGCAC ACATGGGTCC TGGAGATGGA ATACAGATCC TGAAAGATCT GCCCATGAAC 
CCTCGGCCTC AGGAAAGTCG GACAGTATTC AGTGGCTCAG TCAGTATTCC ATCTATCACC 
AAATCCCGCC CTGAGCCAGG CCGCTCCATG AGTGCTAGCA GTGGCTTGTC AGCAAGGTCA 
TCCGCACAGA ATGGCAGCGC ATTAAAGAGG GAATTCTCTG GAGGAAGCTA CAGTGCCAAG 
CGGCAGCCCA TGCCCTCCCC GTCAGAGGGC TCTTTGTCCT CTGGAGGCAT GGACCAAGGA 
AGTGATGCCC CAGCGAGGGA CTTTGACGGA GAGGACTCTG ACTCTCCGAG GCATTCCACA 
GCTTCCAACA GTTCCAACCT AAGCAGCCCC CCAAGCCCAG CTTCACCCCG AAAAACCAAG 
AGCCTCTCCC TGGAGAGCAC TGACCGCGGG AGCTGGGACC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_005273322.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005273379.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X12, mRNA.

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CloneID	OHu35144
Clone ID Related Accession (Same CDS sequence)	XM_005273322.4 , XM_005273322.3
Accession Version	XM_005273322.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005273322.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGAT CCCGTTGAGA ACACATATGT ATGGAACCCG 
AGCGTCAAGT TTCACATCCA GTCACGGTCC ACATCTCCTT CCACATCTAG TGAAGCTGAG 
CCAGTTAAGA CTGTAGACTC CACTCCACTT TCAGTTCACA CACCAACCTT AAGGAAAAAA 
GGATGTCCTG GTTCAACTGG CTTTCCACCT AAGCGCAAGA CTCACCAGTT TTTTGTAAAA 
TCTTTTACTA CTCCTACCAA GTGTCATCAG TGTACCTCCT TGATGGTGGG TTTAATAAGA 
CAGGGCTGTT CATGTGAAGT GTGTGGATTC TCATGCCATA TAACTTGTGT AAACAAAGCT 
CCAACCACTT GTCCAGTTCC TCCTGAACAG ACAAAAGGTC CCCTGGGTAT AGATCCTCAG 
AAAGGAATAG GAACAGCATA TGAAGGTCAT GTCAGGATTC CTAAGCCAGC TGGAGTGAAG 
AAAGGGTGGC AGAGAGCACT GGCTATAGTG TGTGACTTCA AACTCTTTCT GTACGATATT 
GCTGAAGGAA AAGCATCTCA GCCCAGTGTT GTCATTAGTC AAGTGATTGA CATGAGGGAT 
GAAGAATTTT CTGTGAGTTC AGTCTTGGCT TCTGATGTTA TCCATGCAAG TCGGAAAGAT 
ATACCCTGTA TATTTAGGGT CACAGCTTCC CAGCTCTCAG CATCTAATAA CAAATGTTCA 
ATCCTGATGC TAGCAGACAC TGAGAATGAG AAGAATAAGT GGGTGGGAGT GCTGAGTGAA 
TTGCACAAGA TTTTGAAGAA AAACAAATTC AGAGACCGCT CAGTCTATGT TCCCAAAGAG 
GCTTATGACA GCACTCTACC CCTCATTAAA ACAACCCAGG CAGCCGCAAT CATAGATCAT 
GAAAGAATTG CTTTGGGAAA CGAAGAAGGG TTATTTGTTG TACATGTCAC CAAAGATGAA 
ATTATTAGAG TTGGTGACAA TAAGAAGATT CATCAGATTG AACTCATTCC AAATGATCAG 
CTTGTTGCTG TGATCTCAGG ACGAAATCGT CATGTACGAC TTTTTCCTAT GTCAGCATTG 
GATGGGCGAG AGACCGATTT TTACAAGCTG TCAGAAACTA AAGGGTGTCA AACCGTAACT 
TCTGGAAAGG TGCGCCATGG AGCTCTCACA TGCCTGTGTG TGGCTATGAA AAGGCAGGTC 
CTCTGTTATG AACTATTTCA GAGCAAGACC CGTCACAGAA AATTTAAAGA AATTCAAGTC 
CCATATAATG TCCAGTGGAT GGCAATCTTC AGTGAACAAC TCTGTGTGGG ATTCCAGTCA 
GGATTTCTAA GATACCCCTT GAATGGAGAA GGAAATCCAT ACAGTATGCT CCATTCAAAT 
GACCATACAC TATCATTTAT TGCACATCAA CCAATGGATG CTATCTGCGC AGTTGAGATC 
TCCAGTAAAG AATATCTGCT GTGTTTTAAC AGCATTGGGA TATACACTGA CTGCCAGGGC 
CGAAGATCTA GACAACAGGA ATTGATGTGG CCAGCAAATC CTTCCTCTTG TTGTTACAAT 
GCACCATATC TCTCGGTGTA CAGTGAAAAT GCAGTTGATA TCTTTGATGT GAACTCCATG 
GAATGGATTC AGACTCTTCC TCTCAAAAAG GTTCGACCCT TAAACAATGA AGGATCATTA 
AATCTTTTAG GGTTGGAGAC CATTAGATTA ATATATTTCA AAAATAAGAT GGCAGAAGGG 
GACGAACTGG TAGTACCTGA AACATCAGAT AATAGTCGGA AACAAATGGT TAGAAACATT 
AACAATAAGC GGCGTTATTC CTTCAGAGTC CCAGAAGAGG AAAGGATGCA GCAGAGGAGG 
GAAATGCTAC GAGATCCAGA AATGAGAAAT AAATTAATTT CTAATCCAAC TAATTTTAAT 
CACATAGCAC ACATGGGTCC TGGAGATGGA ATACAGATCC TGAAAGATCT GCCCATGAAC 
CCTCGGCCTC AGGAAAGTCG GACAGTATTC AGTGGCTCAG TCAGTATTCC ATCTATCACC 
AAATCCCGCC CTGAGCCAGG CCGCTCCATG AGTGCTAGCA GTGGCTTGTC AGCAAGGTCA 
TCCGCACAGA ATGGCAGCGC ATTAAAGAGG GAATTCTCTG GAGGAAGCTA CAGTGCCAAG 
CGGCAGCCCA TGCCCTCCCC GTCAGAGGGC TCTTTGTCCT CTGGAGGCAT GGACCAAGGA 
AGTGATGCCC CAGCGAGGGA CTTTGACGGA GAGGACTCTG ACTCTCCGAG GCATTCCACA 
GCTTCCAACA GTTCCAACCT AAGCAGCCCC CCAAGCCCAG CTTCACCCCG AAAAACCAAG 
AGCCTCTCCC TGGAGAGCAC TGACCGCGGG AGCTGGGACC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005273379.1
CDS	1056..6320
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETDPVENTYVWNPSVKFHIQSRSTSPSTSSEAEPVKTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_005273322.3
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005273322.3

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGAT CCCGTTGAGA ACACATATGT ATGGAACCCG 
AGCGTCAAGT TTCACATCCA GTCACGGTCC ACATCTCCTT CCACATCTAG TGAAGCTGAG 
CCAGTTAAGA CTGTAGACTC CACTCCACTT TCAGTTCACA CACCAACCTT AAGGAAAAAA 
GGATGTCCTG GTTCAACTGG CTTTCCACCT AAGCGCAAGA CTCACCAGTT TTTTGTAAAA 
TCTTTTACTA CTCCTACCAA GTGTCATCAG TGTACCTCCT TGATGGTGGG TTTAATAAGA 
CAGGGCTGTT CATGTGAAGT GTGTGGATTC TCATGCCATA TAACTTGTGT AAACAAAGCT 
CCAACCACTT GTCCAGTTCC TCCTGAACAG ACAAAAGGTC CCCTGGGTAT AGATCCTCAG 
AAAGGAATAG GAACAGCATA TGAAGGTCAT GTCAGGATTC CTAAGCCAGC TGGAGTGAAG 
AAAGGGTGGC AGAGAGCACT GGCTATAGTG TGTGACTTCA AACTCTTTCT GTACGATATT 
GCTGAAGGAA AAGCATCTCA GCCCAGTGTT GTCATTAGTC AAGTGATTGA CATGAGGGAT 
GAAGAATTTT CTGTGAGTTC AGTCTTGGCT TCTGATGTTA TCCATGCAAG TCGGAAAGAT 
ATACCCTGTA TATTTAGGGT CACAGCTTCC CAGCTCTCAG CATCTAATAA CAAATGTTCA 
ATCCTGATGC TAGCAGACAC TGAGAATGAG AAGAATAAGT GGGTGGGAGT GCTGAGTGAA 
TTGCACAAGA TTTTGAAGAA AAACAAATTC AGAGACCGCT CAGTCTATGT TCCCAAAGAG 
GCTTATGACA GCACTCTACC CCTCATTAAA ACAACCCAGG CAGCCGCAAT CATAGATCAT 
GAAAGAATTG CTTTGGGAAA CGAAGAAGGG TTATTTGTTG TACATGTCAC CAAAGATGAA 
ATTATTAGAG TTGGTGACAA TAAGAAGATT CATCAGATTG AACTCATTCC AAATGATCAG 
CTTGTTGCTG TGATCTCAGG ACGAAATCGT CATGTACGAC TTTTTCCTAT GTCAGCATTG 
GATGGGCGAG AGACCGATTT TTACAAGCTG TCAGAAACTA AAGGGTGTCA AACCGTAACT 
TCTGGAAAGG TGCGCCATGG AGCTCTCACA TGCCTGTGTG TGGCTATGAA AAGGCAGGTC 
CTCTGTTATG AACTATTTCA GAGCAAGACC CGTCACAGAA AATTTAAAGA AATTCAAGTC 
CCATATAATG TCCAGTGGAT GGCAATCTTC AGTGAACAAC TCTGTGTGGG ATTCCAGTCA 
GGATTTCTAA GATACCCCTT GAATGGAGAA GGAAATCCAT ACAGTATGCT CCATTCAAAT 
GACCATACAC TATCATTTAT TGCACATCAA CCAATGGATG CTATCTGCGC AGTTGAGATC 
TCCAGTAAAG AATATCTGCT GTGTTTTAAC AGCATTGGGA TATACACTGA CTGCCAGGGC 
CGAAGATCTA GACAACAGGA ATTGATGTGG CCAGCAAATC CTTCCTCTTG TTGTTACAAT 
GCACCATATC TCTCGGTGTA CAGTGAAAAT GCAGTTGATA TCTTTGATGT GAACTCCATG 
GAATGGATTC AGACTCTTCC TCTCAAAAAG GTTCGACCCT TAAACAATGA AGGATCATTA 
AATCTTTTAG GGTTGGAGAC CATTAGATTA ATATATTTCA AAAATAAGAT GGCAGAAGGG 
GACGAACTGG TAGTACCTGA AACATCAGAT AATAGTCGGA AACAAATGGT TAGAAACATT 
AACAATAAGC GGCGTTATTC CTTCAGAGTC CCAGAAGAGG AAAGGATGCA GCAGAGGAGG 
GAAATGCTAC GAGATCCAGA AATGAGAAAT AAATTAATTT CTAATCCAAC TAATTTTAAT 
CACATAGCAC ACATGGGTCC TGGAGATGGA ATACAGATCC TGAAAGATCT GCCCATGAAC 
CCTCGGCCTC AGGAAAGTCG GACAGTATTC AGTGGCTCAG TCAGTATTCC ATCTATCACC 
AAATCCCGCC CTGAGCCAGG CCGCTCCATG AGTGCTAGCA GTGGCTTGTC AGCAAGGTCA 
TCCGCACAGA ATGGCAGCGC ATTAAAGAGG GAATTCTCTG GAGGAAGCTA CAGTGCCAAG 
CGGCAGCCCA TGCCCTCCCC GTCAGAGGGC TCTTTGTCCT CTGGAGGCAT GGACCAAGGA 
AGTGATGCCC CAGCGAGGGA CTTTGACGGA GAGGACTCTG ACTCTCCGAG GCATTCCACA 
GCTTCCAACA GTTCCAACCT AAGCAGCCCC CCAAGCCCAG CTTCACCCCG AAAAACCAAG 
AGCCTCTCCC TGGAGAGCAC TGACCGCGGG AGCTGGGACC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_005273324.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005273381.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X15, mRNA.

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CloneID	OHu35146
Clone ID Related Accession (Same CDS sequence)	NM_001366019.1 , NM_001366019.2 , XM_005273324.3
Accession Version	XM_005273324.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005273324.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGTAG ACTCCACTCC ACTTTCAGTT CACACACCAA CCTTAAGGAA AAAAGGATGT 
CCTGGTTCAA CTGGCTTTCC ACCTAAGCGC AAGACTCACC AGTTTTTTGT AAAATCTTTT 
ACTACTCCTA CCAAGTGTCA TCAGTGTACC TCCTTGATGG TGGGTTTAAT AAGACAGGGC 
TGTTCATGTG AAGTGTGTGG ATTCTCATGC CATATAACTT GTGTAAACAA AGCTCCAACC 
ACTTGTCCAG TTCCTCCTGA ACAGACAAAA GGTCCCCTGG GTATAGATCC TCAGAAAGGA 
ATAGGAACAG CATATGAAGG TCATGTCAGG ATTCCTAAGC CAGCTGGAGT GAAGAAAGGG 
TGGCAGAGAG CACTGGCTAT AGTGTGTGAC TTCAAACTCT TTCTGTACGA TATTGCTGAA 
GGAAAAGCAT CTCAGCCCAG TGTTGTCATT AGTCAAGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT GTTATCCATG CAAGTCGGAA AGATATACCC 
TGTATATTTA GGGTCACAGC TTCCCAGCTC TCAGCATCTA ATAACAAATG TTCAATCCTG 
ATGCTAGCAG ACACTGAGAA TGAGAAGAAT AAGTGGGTGG GAGTGCTGAG TGAATTGCAC 
AAGATTTTGA AGAAAAACAA ATTCAGAGAC CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT 
GACAGCACTC TACCCCTCAT TAAAACAACC CAGGCAGCCG CAATCATAGA TCATGAAAGA 
ATTGCTTTGG GAAACGAAGA AGGGTTATTT GTTGTACATG TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ACAATAAGAA GATTCATCAG ATTGAACTCA TTCCAAATGA TCAGCTTGTT 
GCTGTGATCT CAGGACGAAA TCGTCATGTA CGACTTTTTC CTATGTCAGC ATTGGATGGG 
CGAGAGACCG ATTTTTACAA GCTGTCAGAA ACTAAAGGGT GTCAAACCGT AACTTCTGGA 
AAGGTGCGCC ATGGAGCTCT CACATGCCTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACCCGTCAC AGAAAATTTA AAGAAATTCA AGTCCCATAT 
AATGTCCAGT GGATGGCAAT CTTCAGTGAA CAACTCTGTG TGGGATTCCA GTCAGGATTT 
CTAAGATACC CCTTGAATGG AGAAGGAAAT CCATACAGTA TGCTCCATTC AAATGACCAT 
ACACTATCAT TTATTGCACA TCAACCAATG GATGCTATCT GCGCAGTTGA GATCTCCAGT 
AAAGAATATC TGCTGTGTTT TAACAGCATT GGGATATACA CTGACTGCCA GGGCCGAAGA 
TCTAGACAAC AGGAATTGAT GTGGCCAGCA AATCCTTCCT CTTGTTGTTA CAATGCACCA 
TATCTCTCGG TGTACAGTGA AAATGCAGTT GATATCTTTG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TTCCTCTCAA AAAGGTTCGA CCCTTAAACA ATGAAGGATC ATTAAATCTT 
TTAGGGTTGG AGACCATTAG ATTAATATAT TTCAAAAATA AGATGGCAGA AGGGGACGAA 
CTGGTAGTAC CTGAAACATC AGATAATAGT CGGAAACAAA TGGTTAGAAA CATTAACAAT 
AAGCGGCGTT ATTCCTTCAG AGTCCCAGAA GAGGAAAGGA TGCAGCAGAG GAGGGAAATG 
CTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAATC CAACTAATTT TAATCACATA 
GCACACATGG GTCCTGGAGA TGGAATACAG ATCCTGAAAG ATCTGCCCAT GAACCCTCGG 
CCTCAGGAAA GTCGGACAGT ATTCAGTGGC TCAGTCAGTA TTCCATCTAT CACCAAATCC 
CGCCCTGAGC CAGGCCGCTC CATGAGTGCT AGCAGTGGCT TGTCAGCAAG GTCATCCGCA 
CAGAATGGCA GCGCATTAAA GAGGGAATTC TCTGGAGGAA GCTACAGTGC CAAGCGGCAG 
CCCATGCCCT CCCCGTCAGA GGGCTCTTTG TCCTCTGGAG GCATGGACCA AGGAAGTGAT 
GCCCCAGCGA GGGACTTTGA CGGAGAGGAC TCTGACTCTC CGAGGCATTC CACAGCTTCC 
AACAGTTCCA ACCTAAGCAG CCCCCCAAGC CCAGCTTCAC CCCGAAAAAC CAAGAGCCTC 
TCCCTGGAGA GCACTGACCG CGGGAGCTGG GACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005273381.1
CDS	1152..6350
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_005273324.3
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005273324.3

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGTAG ACTCCACTCC ACTTTCAGTT CACACACCAA CCTTAAGGAA AAAAGGATGT 
CCTGGTTCAA CTGGCTTTCC ACCTAAGCGC AAGACTCACC AGTTTTTTGT AAAATCTTTT 
ACTACTCCTA CCAAGTGTCA TCAGTGTACC TCCTTGATGG TGGGTTTAAT AAGACAGGGC 
TGTTCATGTG AAGTGTGTGG ATTCTCATGC CATATAACTT GTGTAAACAA AGCTCCAACC 
ACTTGTCCAG TTCCTCCTGA ACAGACAAAA GGTCCCCTGG GTATAGATCC TCAGAAAGGA 
ATAGGAACAG CATATGAAGG TCATGTCAGG ATTCCTAAGC CAGCTGGAGT GAAGAAAGGG 
TGGCAGAGAG CACTGGCTAT AGTGTGTGAC TTCAAACTCT TTCTGTACGA TATTGCTGAA 
GGAAAAGCAT CTCAGCCCAG TGTTGTCATT AGTCAAGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT GTTATCCATG CAAGTCGGAA AGATATACCC 
TGTATATTTA GGGTCACAGC TTCCCAGCTC TCAGCATCTA ATAACAAATG TTCAATCCTG 
ATGCTAGCAG ACACTGAGAA TGAGAAGAAT AAGTGGGTGG GAGTGCTGAG TGAATTGCAC 
AAGATTTTGA AGAAAAACAA ATTCAGAGAC CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT 
GACAGCACTC TACCCCTCAT TAAAACAACC CAGGCAGCCG CAATCATAGA TCATGAAAGA 
ATTGCTTTGG GAAACGAAGA AGGGTTATTT GTTGTACATG TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ACAATAAGAA GATTCATCAG ATTGAACTCA TTCCAAATGA TCAGCTTGTT 
GCTGTGATCT CAGGACGAAA TCGTCATGTA CGACTTTTTC CTATGTCAGC ATTGGATGGG 
CGAGAGACCG ATTTTTACAA GCTGTCAGAA ACTAAAGGGT GTCAAACCGT AACTTCTGGA 
AAGGTGCGCC ATGGAGCTCT CACATGCCTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACCCGTCAC AGAAAATTTA AAGAAATTCA AGTCCCATAT 
AATGTCCAGT GGATGGCAAT CTTCAGTGAA CAACTCTGTG TGGGATTCCA GTCAGGATTT 
CTAAGATACC CCTTGAATGG AGAAGGAAAT CCATACAGTA TGCTCCATTC AAATGACCAT 
ACACTATCAT TTATTGCACA TCAACCAATG GATGCTATCT GCGCAGTTGA GATCTCCAGT 
AAAGAATATC TGCTGTGTTT TAACAGCATT GGGATATACA CTGACTGCCA GGGCCGAAGA 
TCTAGACAAC AGGAATTGAT GTGGCCAGCA AATCCTTCCT CTTGTTGTTA CAATGCACCA 
TATCTCTCGG TGTACAGTGA AAATGCAGTT GATATCTTTG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TTCCTCTCAA AAAGGTTCGA CCCTTAAACA ATGAAGGATC ATTAAATCTT 
TTAGGGTTGG AGACCATTAG ATTAATATAT TTCAAAAATA AGATGGCAGA AGGGGACGAA 
CTGGTAGTAC CTGAAACATC AGATAATAGT CGGAAACAAA TGGTTAGAAA CATTAACAAT 
AAGCGGCGTT ATTCCTTCAG AGTCCCAGAA GAGGAAAGGA TGCAGCAGAG GAGGGAAATG 
CTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAATC CAACTAATTT TAATCACATA 
GCACACATGG GTCCTGGAGA TGGAATACAG ATCCTGAAAG ATCTGCCCAT GAACCCTCGG 
CCTCAGGAAA GTCGGACAGT ATTCAGTGGC TCAGTCAGTA TTCCATCTAT CACCAAATCC 
CGCCCTGAGC CAGGCCGCTC CATGAGTGCT AGCAGTGGCT TGTCAGCAAG GTCATCCGCA 
CAGAATGGCA GCGCATTAAA GAGGGAATTC TCTGGAGGAA GCTACAGTGC CAAGCGGCAG 
CCCATGCCCT CCCCGTCAGA GGGCTCTTTG TCCTCTGGAG GCATGGACCA AGGAAGTGAT 
GCCCCAGCGA GGGACTTTGA CGGAGAGGAC TCTGACTCTC CGAGGCATTC CACAGCTTCC 
AACAGTTCCA ACCTAAGCAG CCCCCCAAGC CCAGCTTCAC CCCGAAAAAC CAAGAGCCTC 
TCCCTGGAGA GCACTGACCG CGGGAGCTGG GACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_011544306.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542608.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X1, mRNA.

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CloneID	OHu61833
Clone ID Related Accession (Same CDS sequence)	XM_011544306.3 , XM_011544306.2
Accession Version	XM_011544306.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011544306.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC ATCCTGTACT CCAGCTAGCA AAGGCAGACG TACTGATCCC 
GTTGAGAACA CATATGTATG GAACCCGAGC GTCAAGTTTC ACATCCAGTC ACGGTCCACA 
TCTCCTTCCA CATCTAGTGA AGCTGAGCCA GTTAAGACTG TAGACTCCAC TCCACTTTCA 
GTTCACACAC CAACCTTAAG GAAAAAAGGA TGTCCTGGTT CAACTGGCTT TCCACCTAAG 
CGCAAGACTC ACCAGTTTTT TGTAAAATCT TTTACTACTC CTACCAAGTG TCATCAGTGT 
ACCTCCTTGA TGGTGGGTTT AATAAGACAG GGCTGTTCAT GTGAAGTGTG TGGATTCTCA 
TGCCATATAA CTTGTGTAAA CAAAGCTCCA ACCACTTGTC CAGTTCCTCC TGAACAGACA 
AAAGGTCCCC TGGGTATAGA TCCTCAGAAA GGAATAGGAA CAGCATATGA AGGTCATGTC 
AGGATTCCTA AGCCAGCTGG AGTGAAGAAA GGGTGGCAGA GAGCACTGGC TATAGTGTGT 
GACTTCAAAC TCTTTCTGTA CGATATTGCT GAAGGAAAAG CATCTCAGCC CAGTGTTGTC 
ATTAGTCAAG TGATTGACAT GAGGGATGAA GAATTTTCTG TGAGTTCAGT CTTGGCTTCT 
GATGTTATCC ATGCAAGTCG GAAAGATATA CCCTGTATAT TTAGGGTCAC AGCTTCCCAG 
CTCTCAGCAT CTAATAACAA ATGTTCAATC CTGATGCTAG CAGACACTGA GAATGAGAAG 
AATAAGTGGG TGGGAGTGCT GAGTGAATTG CACAAGATTT TGAAGAAAAA CAAATTCAGA 
GACCGCTCAG TCTATGTTCC CAAAGAGGCT TATGACAGCA CTCTACCCCT CATTAAAACA 
ACCCAGGCAG CCGCAATCAT AGATCATGAA AGAATTGCTT TGGGAAACGA AGAAGGGTTA 
TTTGTTGTAC ATGTCACCAA AGATGAAATT ATTAGAGTTG GTGACAATAA GAAGATTCAT 
CAGATTGAAC TCATTCCAAA TGATCAGCTT GTTGCTGTGA TCTCAGGACG AAATCGTCAT 
GTACGACTTT TTCCTATGTC AGCATTGGAT GGGCGAGAGA CCGATTTTTA CAAGCTGTCA 
GAAACTAAAG GGTGTCAAAC CGTAACTTCT GGAAAGGTGC GCCATGGAGC TCTCACATGC 
CTGTGTGTGG CTATGAAAAG GCAGGTCCTC TGTTATGAAC TATTTCAGAG CAAGACCCGT 
CACAGAAAAT TTAAAGAAAT TCAAGTCCCA TATAATGTCC AGTGGATGGC AATCTTCAGT 
GAACAACTCT GTGTGGGATT CCAGTCAGGA TTTCTAAGAT ACCCCTTGAA TGGAGAAGGA 
AATCCATACA GTATGCTCCA TTCAAATGAC CATACACTAT CATTTATTGC ACATCAACCA 
ATGGATGCTA TCTGCGCAGT TGAGATCTCC AGTAAAGAAT ATCTGCTGTG TTTTAACAGC 
ATTGGGATAT ACACTGACTG CCAGGGCCGA AGATCTAGAC AACAGGAATT GATGTGGCCA 
GCAAATCCTT CCTCTTGTTG TTACAATGCA CCATATCTCT CGGTGTACAG TGAAAATGCA 
GTTGATATCT TTGATGTGAA CTCCATGGAA TGGATTCAGA CTCTTCCTCT CAAAAAGGTT 
CGACCCTTAA ACAATGAAGG ATCATTAAAT CTTTTAGGGT TGGAGACCAT TAGATTAATA 
TATTTCAAAA ATAAGATGGC AGAAGGGGAC GAACTGGTAG TACCTGAAAC ATCAGATAAT 
AGTCGGAAAC AAATGGTTAG AAACATTAAC AATAAGCGGC GTTATTCCTT CAGAGTCCCA 
GAAGAGGAAA GGATGCAGCA GAGGAGGGAA ATGCTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ATCCAACTAA TTTTAATCAC ATAGCACACA TGGGTCCTGG AGATGGAATA 
CAGATCCTGA AAGATCTGCC CATGCCAGGT TTCCCTTATC CATCCCCTCA TCATCACTCC 
GGTCTGATCT CCTCTCCGAT CAACTTTGAA CACATCTATC ACATGACTGT TAATTCTGCT 
GAAAAATTTC TCTCTCCTGA TTCCATAAAC CCTGAATATT CCCCGTCTCT GCGCTCTGTC 
CCTGGTACAC CAAGCTTTAT GACTCTGAGG AACCCTCGGC CTCAGGAAAG TCGGACAGTA 
TTCAGTGGCT CAGTCAGTAT TCCATCTATC ACCAAATCCC GCCCTGAGCC AGGCCGCTCC 
ATGAGTGCTA GCAGTGGCTT GTCAGCAAGG TCATCCGCAC AGAATGGCAG CGCATTAAAG 
AGGGAATTCT CTGGAGGAAG CTACAGTGCC AAGCGGCAGC CCATGCCCTC CCCGTCAGAG 
GGCTCTTTGT CCTCTGGAGG CATGGACCAA GGAAGTGATG CCCCAGCGAG GGACTTTGAC 
GGAGAGGACT CTGACTCTCC GAGGCATTCC ACAGCTTCCA ACAGTTCCAA CCTAAGCAGC 
CCCCCAAGCC CAGCTTCACC CCGAAAAACC AAGAGCCTCT CCCTGGAGAG CACTGACCGC 
GGGAGCTGGG ACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542608.1
CDS	1321..6858
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVENTYVWNPSVKFHIQSRSTSPSTSSEAEPVKTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_011544306.3
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544306.3

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC ATCCTGTACT CCAGCTAGCA AAGGCAGACG TACTGATCCC 
GTTGAGAACA CATATGTATG GAACCCGAGC GTCAAGTTTC ACATCCAGTC ACGGTCCACA 
TCTCCTTCCA CATCTAGTGA AGCTGAGCCA GTTAAGACTG TAGACTCCAC TCCACTTTCA 
GTTCACACAC CAACCTTAAG GAAAAAAGGA TGTCCTGGTT CAACTGGCTT TCCACCTAAG 
CGCAAGACTC ACCAGTTTTT TGTAAAATCT TTTACTACTC CTACCAAGTG TCATCAGTGT 
ACCTCCTTGA TGGTGGGTTT AATAAGACAG GGCTGTTCAT GTGAAGTGTG TGGATTCTCA 
TGCCATATAA CTTGTGTAAA CAAAGCTCCA ACCACTTGTC CAGTTCCTCC TGAACAGACA 
AAAGGTCCCC TGGGTATAGA TCCTCAGAAA GGAATAGGAA CAGCATATGA AGGTCATGTC 
AGGATTCCTA AGCCAGCTGG AGTGAAGAAA GGGTGGCAGA GAGCACTGGC TATAGTGTGT 
GACTTCAAAC TCTTTCTGTA CGATATTGCT GAAGGAAAAG CATCTCAGCC CAGTGTTGTC 
ATTAGTCAAG TGATTGACAT GAGGGATGAA GAATTTTCTG TGAGTTCAGT CTTGGCTTCT 
GATGTTATCC ATGCAAGTCG GAAAGATATA CCCTGTATAT TTAGGGTCAC AGCTTCCCAG 
CTCTCAGCAT CTAATAACAA ATGTTCAATC CTGATGCTAG CAGACACTGA GAATGAGAAG 
AATAAGTGGG TGGGAGTGCT GAGTGAATTG CACAAGATTT TGAAGAAAAA CAAATTCAGA 
GACCGCTCAG TCTATGTTCC CAAAGAGGCT TATGACAGCA CTCTACCCCT CATTAAAACA 
ACCCAGGCAG CCGCAATCAT AGATCATGAA AGAATTGCTT TGGGAAACGA AGAAGGGTTA 
TTTGTTGTAC ATGTCACCAA AGATGAAATT ATTAGAGTTG GTGACAATAA GAAGATTCAT 
CAGATTGAAC TCATTCCAAA TGATCAGCTT GTTGCTGTGA TCTCAGGACG AAATCGTCAT 
GTACGACTTT TTCCTATGTC AGCATTGGAT GGGCGAGAGA CCGATTTTTA CAAGCTGTCA 
GAAACTAAAG GGTGTCAAAC CGTAACTTCT GGAAAGGTGC GCCATGGAGC TCTCACATGC 
CTGTGTGTGG CTATGAAAAG GCAGGTCCTC TGTTATGAAC TATTTCAGAG CAAGACCCGT 
CACAGAAAAT TTAAAGAAAT TCAAGTCCCA TATAATGTCC AGTGGATGGC AATCTTCAGT 
GAACAACTCT GTGTGGGATT CCAGTCAGGA TTTCTAAGAT ACCCCTTGAA TGGAGAAGGA 
AATCCATACA GTATGCTCCA TTCAAATGAC CATACACTAT CATTTATTGC ACATCAACCA 
ATGGATGCTA TCTGCGCAGT TGAGATCTCC AGTAAAGAAT ATCTGCTGTG TTTTAACAGC 
ATTGGGATAT ACACTGACTG CCAGGGCCGA AGATCTAGAC AACAGGAATT GATGTGGCCA 
GCAAATCCTT CCTCTTGTTG TTACAATGCA CCATATCTCT CGGTGTACAG TGAAAATGCA 
GTTGATATCT TTGATGTGAA CTCCATGGAA TGGATTCAGA CTCTTCCTCT CAAAAAGGTT 
CGACCCTTAA ACAATGAAGG ATCATTAAAT CTTTTAGGGT TGGAGACCAT TAGATTAATA 
TATTTCAAAA ATAAGATGGC AGAAGGGGAC GAACTGGTAG TACCTGAAAC ATCAGATAAT 
AGTCGGAAAC AAATGGTTAG AAACATTAAC AATAAGCGGC GTTATTCCTT CAGAGTCCCA 
GAAGAGGAAA GGATGCAGCA GAGGAGGGAA ATGCTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ATCCAACTAA TTTTAATCAC ATAGCACACA TGGGTCCTGG AGATGGAATA 
CAGATCCTGA AAGATCTGCC CATGCCAGGT TTCCCTTATC CATCCCCTCA TCATCACTCC 
GGTCTGATCT CCTCTCCGAT CAACTTTGAA CACATCTATC ACATGACTGT TAATTCTGCT 
GAAAAATTTC TCTCTCCTGA TTCCATAAAC CCTGAATATT CCCCGTCTCT GCGCTCTGTC 
CCTGGTACAC CAAGCTTTAT GACTCTGAGG AACCCTCGGC CTCAGGAAAG TCGGACAGTA 
TTCAGTGGCT CAGTCAGTAT TCCATCTATC ACCAAATCCC GCCCTGAGCC AGGCCGCTCC 
ATGAGTGCTA GCAGTGGCTT GTCAGCAAGG TCATCCGCAC AGAATGGCAG CGCATTAAAG 
AGGGAATTCT CTGGAGGAAG CTACAGTGCC AAGCGGCAGC CCATGCCCTC CCCGTCAGAG 
GGCTCTTTGT CCTCTGGAGG CATGGACCAA GGAAGTGATG CCCCAGCGAG GGACTTTGAC 
GGAGAGGACT CTGACTCTCC GAGGCATTCC ACAGCTTCCA ACAGTTCCAA CCTAAGCAGC 
CCCCCAAGCC CAGCTTCACC CCGAAAAACC AAGAGCCTCT CCCTGGAGAG CACTGACCGC 
GGGAGCTGGG ACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_011544306.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542608.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X1, mRNA.

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CloneID	OHu61833
Clone ID Related Accession (Same CDS sequence)	XM_011544306.3 , XM_011544306.2
Accession Version	XM_011544306.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011544306.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC ATCCTGTACT CCAGCTAGCA AAGGCAGACG TACTGATCCC 
GTTGAGAACA CATATGTATG GAACCCGAGC GTCAAGTTTC ACATCCAGTC ACGGTCCACA 
TCTCCTTCCA CATCTAGTGA AGCTGAGCCA GTTAAGACTG TAGACTCCAC TCCACTTTCA 
GTTCACACAC CAACCTTAAG GAAAAAAGGA TGTCCTGGTT CAACTGGCTT TCCACCTAAG 
CGCAAGACTC ACCAGTTTTT TGTAAAATCT TTTACTACTC CTACCAAGTG TCATCAGTGT 
ACCTCCTTGA TGGTGGGTTT AATAAGACAG GGCTGTTCAT GTGAAGTGTG TGGATTCTCA 
TGCCATATAA CTTGTGTAAA CAAAGCTCCA ACCACTTGTC CAGTTCCTCC TGAACAGACA 
AAAGGTCCCC TGGGTATAGA TCCTCAGAAA GGAATAGGAA CAGCATATGA AGGTCATGTC 
AGGATTCCTA AGCCAGCTGG AGTGAAGAAA GGGTGGCAGA GAGCACTGGC TATAGTGTGT 
GACTTCAAAC TCTTTCTGTA CGATATTGCT GAAGGAAAAG CATCTCAGCC CAGTGTTGTC 
ATTAGTCAAG TGATTGACAT GAGGGATGAA GAATTTTCTG TGAGTTCAGT CTTGGCTTCT 
GATGTTATCC ATGCAAGTCG GAAAGATATA CCCTGTATAT TTAGGGTCAC AGCTTCCCAG 
CTCTCAGCAT CTAATAACAA ATGTTCAATC CTGATGCTAG CAGACACTGA GAATGAGAAG 
AATAAGTGGG TGGGAGTGCT GAGTGAATTG CACAAGATTT TGAAGAAAAA CAAATTCAGA 
GACCGCTCAG TCTATGTTCC CAAAGAGGCT TATGACAGCA CTCTACCCCT CATTAAAACA 
ACCCAGGCAG CCGCAATCAT AGATCATGAA AGAATTGCTT TGGGAAACGA AGAAGGGTTA 
TTTGTTGTAC ATGTCACCAA AGATGAAATT ATTAGAGTTG GTGACAATAA GAAGATTCAT 
CAGATTGAAC TCATTCCAAA TGATCAGCTT GTTGCTGTGA TCTCAGGACG AAATCGTCAT 
GTACGACTTT TTCCTATGTC AGCATTGGAT GGGCGAGAGA CCGATTTTTA CAAGCTGTCA 
GAAACTAAAG GGTGTCAAAC CGTAACTTCT GGAAAGGTGC GCCATGGAGC TCTCACATGC 
CTGTGTGTGG CTATGAAAAG GCAGGTCCTC TGTTATGAAC TATTTCAGAG CAAGACCCGT 
CACAGAAAAT TTAAAGAAAT TCAAGTCCCA TATAATGTCC AGTGGATGGC AATCTTCAGT 
GAACAACTCT GTGTGGGATT CCAGTCAGGA TTTCTAAGAT ACCCCTTGAA TGGAGAAGGA 
AATCCATACA GTATGCTCCA TTCAAATGAC CATACACTAT CATTTATTGC ACATCAACCA 
ATGGATGCTA TCTGCGCAGT TGAGATCTCC AGTAAAGAAT ATCTGCTGTG TTTTAACAGC 
ATTGGGATAT ACACTGACTG CCAGGGCCGA AGATCTAGAC AACAGGAATT GATGTGGCCA 
GCAAATCCTT CCTCTTGTTG TTACAATGCA CCATATCTCT CGGTGTACAG TGAAAATGCA 
GTTGATATCT TTGATGTGAA CTCCATGGAA TGGATTCAGA CTCTTCCTCT CAAAAAGGTT 
CGACCCTTAA ACAATGAAGG ATCATTAAAT CTTTTAGGGT TGGAGACCAT TAGATTAATA 
TATTTCAAAA ATAAGATGGC AGAAGGGGAC GAACTGGTAG TACCTGAAAC ATCAGATAAT 
AGTCGGAAAC AAATGGTTAG AAACATTAAC AATAAGCGGC GTTATTCCTT CAGAGTCCCA 
GAAGAGGAAA GGATGCAGCA GAGGAGGGAA ATGCTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ATCCAACTAA TTTTAATCAC ATAGCACACA TGGGTCCTGG AGATGGAATA 
CAGATCCTGA AAGATCTGCC CATGCCAGGT TTCCCTTATC CATCCCCTCA TCATCACTCC 
GGTCTGATCT CCTCTCCGAT CAACTTTGAA CACATCTATC ACATGACTGT TAATTCTGCT 
GAAAAATTTC TCTCTCCTGA TTCCATAAAC CCTGAATATT CCCCGTCTCT GCGCTCTGTC 
CCTGGTACAC CAAGCTTTAT GACTCTGAGG AACCCTCGGC CTCAGGAAAG TCGGACAGTA 
TTCAGTGGCT CAGTCAGTAT TCCATCTATC ACCAAATCCC GCCCTGAGCC AGGCCGCTCC 
ATGAGTGCTA GCAGTGGCTT GTCAGCAAGG TCATCCGCAC AGAATGGCAG CGCATTAAAG 
AGGGAATTCT CTGGAGGAAG CTACAGTGCC AAGCGGCAGC CCATGCCCTC CCCGTCAGAG 
GGCTCTTTGT CCTCTGGAGG CATGGACCAA GGAAGTGATG CCCCAGCGAG GGACTTTGAC 
GGAGAGGACT CTGACTCTCC GAGGCATTCC ACAGCTTCCA ACAGTTCCAA CCTAAGCAGC 
CCCCCAAGCC CAGCTTCACC CCGAAAAACC AAGAGCCTCT CCCTGGAGAG CACTGACCGC 
GGGAGCTGGG ACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542608.1
CDS	1311..6848
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVENTYVWNPSVKFHIQSRSTSPSTSSEAEPVKTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_011544306.2
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544306.2

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC ATCCTGTACT CCAGCTAGCA AAGGCAGACG TACTGATCCC 
GTTGAGAACA CATATGTATG GAACCCGAGC GTCAAGTTTC ACATCCAGTC ACGGTCCACA 
TCTCCTTCCA CATCTAGTGA AGCTGAGCCA GTTAAGACTG TAGACTCCAC TCCACTTTCA 
GTTCACACAC CAACCTTAAG GAAAAAAGGA TGTCCTGGTT CAACTGGCTT TCCACCTAAG 
CGCAAGACTC ACCAGTTTTT TGTAAAATCT TTTACTACTC CTACCAAGTG TCATCAGTGT 
ACCTCCTTGA TGGTGGGTTT AATAAGACAG GGCTGTTCAT GTGAAGTGTG TGGATTCTCA 
TGCCATATAA CTTGTGTAAA CAAAGCTCCA ACCACTTGTC CAGTTCCTCC TGAACAGACA 
AAAGGTCCCC TGGGTATAGA TCCTCAGAAA GGAATAGGAA CAGCATATGA AGGTCATGTC 
AGGATTCCTA AGCCAGCTGG AGTGAAGAAA GGGTGGCAGA GAGCACTGGC TATAGTGTGT 
GACTTCAAAC TCTTTCTGTA CGATATTGCT GAAGGAAAAG CATCTCAGCC CAGTGTTGTC 
ATTAGTCAAG TGATTGACAT GAGGGATGAA GAATTTTCTG TGAGTTCAGT CTTGGCTTCT 
GATGTTATCC ATGCAAGTCG GAAAGATATA CCCTGTATAT TTAGGGTCAC AGCTTCCCAG 
CTCTCAGCAT CTAATAACAA ATGTTCAATC CTGATGCTAG CAGACACTGA GAATGAGAAG 
AATAAGTGGG TGGGAGTGCT GAGTGAATTG CACAAGATTT TGAAGAAAAA CAAATTCAGA 
GACCGCTCAG TCTATGTTCC CAAAGAGGCT TATGACAGCA CTCTACCCCT CATTAAAACA 
ACCCAGGCAG CCGCAATCAT AGATCATGAA AGAATTGCTT TGGGAAACGA AGAAGGGTTA 
TTTGTTGTAC ATGTCACCAA AGATGAAATT ATTAGAGTTG GTGACAATAA GAAGATTCAT 
CAGATTGAAC TCATTCCAAA TGATCAGCTT GTTGCTGTGA TCTCAGGACG AAATCGTCAT 
GTACGACTTT TTCCTATGTC AGCATTGGAT GGGCGAGAGA CCGATTTTTA CAAGCTGTCA 
GAAACTAAAG GGTGTCAAAC CGTAACTTCT GGAAAGGTGC GCCATGGAGC TCTCACATGC 
CTGTGTGTGG CTATGAAAAG GCAGGTCCTC TGTTATGAAC TATTTCAGAG CAAGACCCGT 
CACAGAAAAT TTAAAGAAAT TCAAGTCCCA TATAATGTCC AGTGGATGGC AATCTTCAGT 
GAACAACTCT GTGTGGGATT CCAGTCAGGA TTTCTAAGAT ACCCCTTGAA TGGAGAAGGA 
AATCCATACA GTATGCTCCA TTCAAATGAC CATACACTAT CATTTATTGC ACATCAACCA 
ATGGATGCTA TCTGCGCAGT TGAGATCTCC AGTAAAGAAT ATCTGCTGTG TTTTAACAGC 
ATTGGGATAT ACACTGACTG CCAGGGCCGA AGATCTAGAC AACAGGAATT GATGTGGCCA 
GCAAATCCTT CCTCTTGTTG TTACAATGCA CCATATCTCT CGGTGTACAG TGAAAATGCA 
GTTGATATCT TTGATGTGAA CTCCATGGAA TGGATTCAGA CTCTTCCTCT CAAAAAGGTT 
CGACCCTTAA ACAATGAAGG ATCATTAAAT CTTTTAGGGT TGGAGACCAT TAGATTAATA 
TATTTCAAAA ATAAGATGGC AGAAGGGGAC GAACTGGTAG TACCTGAAAC ATCAGATAAT 
AGTCGGAAAC AAATGGTTAG AAACATTAAC AATAAGCGGC GTTATTCCTT CAGAGTCCCA 
GAAGAGGAAA GGATGCAGCA GAGGAGGGAA ATGCTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ATCCAACTAA TTTTAATCAC ATAGCACACA TGGGTCCTGG AGATGGAATA 
CAGATCCTGA AAGATCTGCC CATGCCAGGT TTCCCTTATC CATCCCCTCA TCATCACTCC 
GGTCTGATCT CCTCTCCGAT CAACTTTGAA CACATCTATC ACATGACTGT TAATTCTGCT 
GAAAAATTTC TCTCTCCTGA TTCCATAAAC CCTGAATATT CCCCGTCTCT GCGCTCTGTC 
CCTGGTACAC CAAGCTTTAT GACTCTGAGG AACCCTCGGC CTCAGGAAAG TCGGACAGTA 
TTCAGTGGCT CAGTCAGTAT TCCATCTATC ACCAAATCCC GCCCTGAGCC AGGCCGCTCC 
ATGAGTGCTA GCAGTGGCTT GTCAGCAAGG TCATCCGCAC AGAATGGCAG CGCATTAAAG 
AGGGAATTCT CTGGAGGAAG CTACAGTGCC AAGCGGCAGC CCATGCCCTC CCCGTCAGAG 
GGCTCTTTGT CCTCTGGAGG CATGGACCAA GGAAGTGATG CCCCAGCGAG GGACTTTGAC 
GGAGAGGACT CTGACTCTCC GAGGCATTCC ACAGCTTCCA ACAGTTCCAA CCTAAGCAGC 
CCCCCAAGCC CAGCTTCACC CCGAAAAACC AAGAGCCTCT CCCTGGAGAG CACTGACCGC 
GGGAGCTGGG ACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_011544307.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542609.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X5, mRNA.

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CloneID	OHu61834
Clone ID Related Accession (Same CDS sequence)	XM_011544307.2 , XM_011544307.3
Accession Version	XM_011544307.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011544307.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC ATCCTGTACT CCAGCTAGCA AAGGCAGACG TACTGTAGAC 
TCCACTCCAC TTTCAGTTCA CACACCAACC TTAAGGAAAA AAGGATGTCC TGGTTCAACT 
GGCTTTCCAC CTAAGCGCAA GACTCACCAG TTTTTTGTAA AATCTTTTAC TACTCCTACC 
AAGTGTCATC AGTGTACCTC CTTGATGGTG GGTTTAATAA GACAGGGCTG TTCATGTGAA 
GTGTGTGGAT TCTCATGCCA TATAACTTGT GTAAACAAAG CTCCAACCAC TTGTCCAGTT 
CCTCCTGAAC AGACAAAAGG TCCCCTGGGT ATAGATCCTC AGAAAGGAAT AGGAACAGCA 
TATGAAGGTC ATGTCAGGAT TCCTAAGCCA GCTGGAGTGA AGAAAGGGTG GCAGAGAGCA 
CTGGCTATAG TGTGTGACTT CAAACTCTTT CTGTACGATA TTGCTGAAGG AAAAGCATCT 
CAGCCCAGTG TTGTCATTAG TCAAGTGATT GACATGAGGG ATGAAGAATT TTCTGTGAGT 
TCAGTCTTGG CTTCTGATGT TATCCATGCA AGTCGGAAAG ATATACCCTG TATATTTAGG 
GTCACAGCTT CCCAGCTCTC AGCATCTAAT AACAAATGTT CAATCCTGAT GCTAGCAGAC 
ACTGAGAATG AGAAGAATAA GTGGGTGGGA GTGCTGAGTG AATTGCACAA GATTTTGAAG 
AAAAACAAAT TCAGAGACCG CTCAGTCTAT GTTCCCAAAG AGGCTTATGA CAGCACTCTA 
CCCCTCATTA AAACAACCCA GGCAGCCGCA ATCATAGATC ATGAAAGAAT TGCTTTGGGA 
AACGAAGAAG GGTTATTTGT TGTACATGTC ACCAAAGATG AAATTATTAG AGTTGGTGAC 
AATAAGAAGA TTCATCAGAT TGAACTCATT CCAAATGATC AGCTTGTTGC TGTGATCTCA 
GGACGAAATC GTCATGTACG ACTTTTTCCT ATGTCAGCAT TGGATGGGCG AGAGACCGAT 
TTTTACAAGC TGTCAGAAAC TAAAGGGTGT CAAACCGTAA CTTCTGGAAA GGTGCGCCAT 
GGAGCTCTCA CATGCCTGTG TGTGGCTATG AAAAGGCAGG TCCTCTGTTA TGAACTATTT 
CAGAGCAAGA CCCGTCACAG AAAATTTAAA GAAATTCAAG TCCCATATAA TGTCCAGTGG 
ATGGCAATCT TCAGTGAACA ACTCTGTGTG GGATTCCAGT CAGGATTTCT AAGATACCCC 
TTGAATGGAG AAGGAAATCC ATACAGTATG CTCCATTCAA ATGACCATAC ACTATCATTT 
ATTGCACATC AACCAATGGA TGCTATCTGC GCAGTTGAGA TCTCCAGTAA AGAATATCTG 
CTGTGTTTTA ACAGCATTGG GATATACACT GACTGCCAGG GCCGAAGATC TAGACAACAG 
GAATTGATGT GGCCAGCAAA TCCTTCCTCT TGTTGTTACA ATGCACCATA TCTCTCGGTG 
TACAGTGAAA ATGCAGTTGA TATCTTTGAT GTGAACTCCA TGGAATGGAT TCAGACTCTT 
CCTCTCAAAA AGGTTCGACC CTTAAACAAT GAAGGATCAT TAAATCTTTT AGGGTTGGAG 
ACCATTAGAT TAATATATTT CAAAAATAAG ATGGCAGAAG GGGACGAACT GGTAGTACCT 
GAAACATCAG ATAATAGTCG GAAACAAATG GTTAGAAACA TTAACAATAA GCGGCGTTAT 
TCCTTCAGAG TCCCAGAAGA GGAAAGGATG CAGCAGAGGA GGGAAATGCT ACGAGATCCA 
GAAATGAGAA ATAAATTAAT TTCTAATCCA ACTAATTTTA ATCACATAGC ACACATGGGT 
CCTGGAGATG GAATACAGAT CCTGAAAGAT CTGCCCATGC CAGGTTTCCC TTATCCATCC 
CCTCATCATC ACTCCGGTCT GATCTCCTCT CCGATCAACT TTGAACACAT CTATCACATG 
ACTGTTAATT CTGCTGAAAA ATTTCTCTCT CCTGATTCCA TAAACCCTGA ATATTCCCCG 
TCTCTGCGCT CTGTCCCTGG TACACCAAGC TTTATGACTC TGAGGAACCC TCGGCCTCAG 
GAAAGTCGGA CAGTATTCAG TGGCTCAGTC AGTATTCCAT CTATCACCAA ATCCCGCCCT 
GAGCCAGGCC GCTCCATGAG TGCTAGCAGT GGCTTGTCAG CAAGGTCATC CGCACAGAAT 
GGCAGCGCAT TAAAGAGGGA ATTCTCTGGA GGAAGCTACA GTGCCAAGCG GCAGCCCATG 
CCCTCCCCGT CAGAGGGCTC TTTGTCCTCT GGAGGCATGG ACCAAGGAAG TGATGCCCCA 
GCGAGGGACT TTGACGGAGA GGACTCTGAC TCTCCGAGGC ATTCCACAGC TTCCAACAGT 
TCCAACCTAA GCAGCCCCCC AAGCCCAGCT TCACCCCGAA AAACCAAGAG CCTCTCCCTG 
GAGAGCACTG ACCGCGGGAG CTGGGACCCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542609.1
CDS	1056..6488
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_011544307.2
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544307.2

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC ATCCTGTACT CCAGCTAGCA AAGGCAGACG TACTGTAGAC 
TCCACTCCAC TTTCAGTTCA CACACCAACC TTAAGGAAAA AAGGATGTCC TGGTTCAACT 
GGCTTTCCAC CTAAGCGCAA GACTCACCAG TTTTTTGTAA AATCTTTTAC TACTCCTACC 
AAGTGTCATC AGTGTACCTC CTTGATGGTG GGTTTAATAA GACAGGGCTG TTCATGTGAA 
GTGTGTGGAT TCTCATGCCA TATAACTTGT GTAAACAAAG CTCCAACCAC TTGTCCAGTT 
CCTCCTGAAC AGACAAAAGG TCCCCTGGGT ATAGATCCTC AGAAAGGAAT AGGAACAGCA 
TATGAAGGTC ATGTCAGGAT TCCTAAGCCA GCTGGAGTGA AGAAAGGGTG GCAGAGAGCA 
CTGGCTATAG TGTGTGACTT CAAACTCTTT CTGTACGATA TTGCTGAAGG AAAAGCATCT 
CAGCCCAGTG TTGTCATTAG TCAAGTGATT GACATGAGGG ATGAAGAATT TTCTGTGAGT 
TCAGTCTTGG CTTCTGATGT TATCCATGCA AGTCGGAAAG ATATACCCTG TATATTTAGG 
GTCACAGCTT CCCAGCTCTC AGCATCTAAT AACAAATGTT CAATCCTGAT GCTAGCAGAC 
ACTGAGAATG AGAAGAATAA GTGGGTGGGA GTGCTGAGTG AATTGCACAA GATTTTGAAG 
AAAAACAAAT TCAGAGACCG CTCAGTCTAT GTTCCCAAAG AGGCTTATGA CAGCACTCTA 
CCCCTCATTA AAACAACCCA GGCAGCCGCA ATCATAGATC ATGAAAGAAT TGCTTTGGGA 
AACGAAGAAG GGTTATTTGT TGTACATGTC ACCAAAGATG AAATTATTAG AGTTGGTGAC 
AATAAGAAGA TTCATCAGAT TGAACTCATT CCAAATGATC AGCTTGTTGC TGTGATCTCA 
GGACGAAATC GTCATGTACG ACTTTTTCCT ATGTCAGCAT TGGATGGGCG AGAGACCGAT 
TTTTACAAGC TGTCAGAAAC TAAAGGGTGT CAAACCGTAA CTTCTGGAAA GGTGCGCCAT 
GGAGCTCTCA CATGCCTGTG TGTGGCTATG AAAAGGCAGG TCCTCTGTTA TGAACTATTT 
CAGAGCAAGA CCCGTCACAG AAAATTTAAA GAAATTCAAG TCCCATATAA TGTCCAGTGG 
ATGGCAATCT TCAGTGAACA ACTCTGTGTG GGATTCCAGT CAGGATTTCT AAGATACCCC 
TTGAATGGAG AAGGAAATCC ATACAGTATG CTCCATTCAA ATGACCATAC ACTATCATTT 
ATTGCACATC AACCAATGGA TGCTATCTGC GCAGTTGAGA TCTCCAGTAA AGAATATCTG 
CTGTGTTTTA ACAGCATTGG GATATACACT GACTGCCAGG GCCGAAGATC TAGACAACAG 
GAATTGATGT GGCCAGCAAA TCCTTCCTCT TGTTGTTACA ATGCACCATA TCTCTCGGTG 
TACAGTGAAA ATGCAGTTGA TATCTTTGAT GTGAACTCCA TGGAATGGAT TCAGACTCTT 
CCTCTCAAAA AGGTTCGACC CTTAAACAAT GAAGGATCAT TAAATCTTTT AGGGTTGGAG 
ACCATTAGAT TAATATATTT CAAAAATAAG ATGGCAGAAG GGGACGAACT GGTAGTACCT 
GAAACATCAG ATAATAGTCG GAAACAAATG GTTAGAAACA TTAACAATAA GCGGCGTTAT 
TCCTTCAGAG TCCCAGAAGA GGAAAGGATG CAGCAGAGGA GGGAAATGCT ACGAGATCCA 
GAAATGAGAA ATAAATTAAT TTCTAATCCA ACTAATTTTA ATCACATAGC ACACATGGGT 
CCTGGAGATG GAATACAGAT CCTGAAAGAT CTGCCCATGC CAGGTTTCCC TTATCCATCC 
CCTCATCATC ACTCCGGTCT GATCTCCTCT CCGATCAACT TTGAACACAT CTATCACATG 
ACTGTTAATT CTGCTGAAAA ATTTCTCTCT CCTGATTCCA TAAACCCTGA ATATTCCCCG 
TCTCTGCGCT CTGTCCCTGG TACACCAAGC TTTATGACTC TGAGGAACCC TCGGCCTCAG 
GAAAGTCGGA CAGTATTCAG TGGCTCAGTC AGTATTCCAT CTATCACCAA ATCCCGCCCT 
GAGCCAGGCC GCTCCATGAG TGCTAGCAGT GGCTTGTCAG CAAGGTCATC CGCACAGAAT 
GGCAGCGCAT TAAAGAGGGA ATTCTCTGGA GGAAGCTACA GTGCCAAGCG GCAGCCCATG 
CCCTCCCCGT CAGAGGGCTC TTTGTCCTCT GGAGGCATGG ACCAAGGAAG TGATGCCCCA 
GCGAGGGACT TTGACGGAGA GGACTCTGAC TCTCCGAGGC ATTCCACAGC TTCCAACAGT 
TCCAACCTAA GCAGCCCCCC AAGCCCAGCT TCACCCCGAA AAACCAAGAG CCTCTCCCTG 
GAGAGCACTG ACCGCGGGAG CTGGGACCCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_011544307.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542609.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu61834
Clone ID Related Accession (Same CDS sequence)	XM_011544307.2 , XM_011544307.3
Accession Version	XM_011544307.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011544307.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC ATCCTGTACT CCAGCTAGCA AAGGCAGACG TACTGTAGAC 
TCCACTCCAC TTTCAGTTCA CACACCAACC TTAAGGAAAA AAGGATGTCC TGGTTCAACT 
GGCTTTCCAC CTAAGCGCAA GACTCACCAG TTTTTTGTAA AATCTTTTAC TACTCCTACC 
AAGTGTCATC AGTGTACCTC CTTGATGGTG GGTTTAATAA GACAGGGCTG TTCATGTGAA 
GTGTGTGGAT TCTCATGCCA TATAACTTGT GTAAACAAAG CTCCAACCAC TTGTCCAGTT 
CCTCCTGAAC AGACAAAAGG TCCCCTGGGT ATAGATCCTC AGAAAGGAAT AGGAACAGCA 
TATGAAGGTC ATGTCAGGAT TCCTAAGCCA GCTGGAGTGA AGAAAGGGTG GCAGAGAGCA 
CTGGCTATAG TGTGTGACTT CAAACTCTTT CTGTACGATA TTGCTGAAGG AAAAGCATCT 
CAGCCCAGTG TTGTCATTAG TCAAGTGATT GACATGAGGG ATGAAGAATT TTCTGTGAGT 
TCAGTCTTGG CTTCTGATGT TATCCATGCA AGTCGGAAAG ATATACCCTG TATATTTAGG 
GTCACAGCTT CCCAGCTCTC AGCATCTAAT AACAAATGTT CAATCCTGAT GCTAGCAGAC 
ACTGAGAATG AGAAGAATAA GTGGGTGGGA GTGCTGAGTG AATTGCACAA GATTTTGAAG 
AAAAACAAAT TCAGAGACCG CTCAGTCTAT GTTCCCAAAG AGGCTTATGA CAGCACTCTA 
CCCCTCATTA AAACAACCCA GGCAGCCGCA ATCATAGATC ATGAAAGAAT TGCTTTGGGA 
AACGAAGAAG GGTTATTTGT TGTACATGTC ACCAAAGATG AAATTATTAG AGTTGGTGAC 
AATAAGAAGA TTCATCAGAT TGAACTCATT CCAAATGATC AGCTTGTTGC TGTGATCTCA 
GGACGAAATC GTCATGTACG ACTTTTTCCT ATGTCAGCAT TGGATGGGCG AGAGACCGAT 
TTTTACAAGC TGTCAGAAAC TAAAGGGTGT CAAACCGTAA CTTCTGGAAA GGTGCGCCAT 
GGAGCTCTCA CATGCCTGTG TGTGGCTATG AAAAGGCAGG TCCTCTGTTA TGAACTATTT 
CAGAGCAAGA CCCGTCACAG AAAATTTAAA GAAATTCAAG TCCCATATAA TGTCCAGTGG 
ATGGCAATCT TCAGTGAACA ACTCTGTGTG GGATTCCAGT CAGGATTTCT AAGATACCCC 
TTGAATGGAG AAGGAAATCC ATACAGTATG CTCCATTCAA ATGACCATAC ACTATCATTT 
ATTGCACATC AACCAATGGA TGCTATCTGC GCAGTTGAGA TCTCCAGTAA AGAATATCTG 
CTGTGTTTTA ACAGCATTGG GATATACACT GACTGCCAGG GCCGAAGATC TAGACAACAG 
GAATTGATGT GGCCAGCAAA TCCTTCCTCT TGTTGTTACA ATGCACCATA TCTCTCGGTG 
TACAGTGAAA ATGCAGTTGA TATCTTTGAT GTGAACTCCA TGGAATGGAT TCAGACTCTT 
CCTCTCAAAA AGGTTCGACC CTTAAACAAT GAAGGATCAT TAAATCTTTT AGGGTTGGAG 
ACCATTAGAT TAATATATTT CAAAAATAAG ATGGCAGAAG GGGACGAACT GGTAGTACCT 
GAAACATCAG ATAATAGTCG GAAACAAATG GTTAGAAACA TTAACAATAA GCGGCGTTAT 
TCCTTCAGAG TCCCAGAAGA GGAAAGGATG CAGCAGAGGA GGGAAATGCT ACGAGATCCA 
GAAATGAGAA ATAAATTAAT TTCTAATCCA ACTAATTTTA ATCACATAGC ACACATGGGT 
CCTGGAGATG GAATACAGAT CCTGAAAGAT CTGCCCATGC CAGGTTTCCC TTATCCATCC 
CCTCATCATC ACTCCGGTCT GATCTCCTCT CCGATCAACT TTGAACACAT CTATCACATG 
ACTGTTAATT CTGCTGAAAA ATTTCTCTCT CCTGATTCCA TAAACCCTGA ATATTCCCCG 
TCTCTGCGCT CTGTCCCTGG TACACCAAGC TTTATGACTC TGAGGAACCC TCGGCCTCAG 
GAAAGTCGGA CAGTATTCAG TGGCTCAGTC AGTATTCCAT CTATCACCAA ATCCCGCCCT 
GAGCCAGGCC GCTCCATGAG TGCTAGCAGT GGCTTGTCAG CAAGGTCATC CGCACAGAAT 
GGCAGCGCAT TAAAGAGGGA ATTCTCTGGA GGAAGCTACA GTGCCAAGCG GCAGCCCATG 
CCCTCCCCGT CAGAGGGCTC TTTGTCCTCT GGAGGCATGG ACCAAGGAAG TGATGCCCCA 
GCGAGGGACT TTGACGGAGA GGACTCTGAC TCTCCGAGGC ATTCCACAGC TTCCAACAGT 
TCCAACCTAA GCAGCCCCCC AAGCCCAGCT TCACCCCGAA AAACCAAGAG CCTCTCCCTG 
GAGAGCACTG ACCGCGGGAG CTGGGACCCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542609.1
CDS	969..6401
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_011544307.3
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544307.3

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC ATCCTGTACT CCAGCTAGCA AAGGCAGACG TACTGTAGAC 
TCCACTCCAC TTTCAGTTCA CACACCAACC TTAAGGAAAA AAGGATGTCC TGGTTCAACT 
GGCTTTCCAC CTAAGCGCAA GACTCACCAG TTTTTTGTAA AATCTTTTAC TACTCCTACC 
AAGTGTCATC AGTGTACCTC CTTGATGGTG GGTTTAATAA GACAGGGCTG TTCATGTGAA 
GTGTGTGGAT TCTCATGCCA TATAACTTGT GTAAACAAAG CTCCAACCAC TTGTCCAGTT 
CCTCCTGAAC AGACAAAAGG TCCCCTGGGT ATAGATCCTC AGAAAGGAAT AGGAACAGCA 
TATGAAGGTC ATGTCAGGAT TCCTAAGCCA GCTGGAGTGA AGAAAGGGTG GCAGAGAGCA 
CTGGCTATAG TGTGTGACTT CAAACTCTTT CTGTACGATA TTGCTGAAGG AAAAGCATCT 
CAGCCCAGTG TTGTCATTAG TCAAGTGATT GACATGAGGG ATGAAGAATT TTCTGTGAGT 
TCAGTCTTGG CTTCTGATGT TATCCATGCA AGTCGGAAAG ATATACCCTG TATATTTAGG 
GTCACAGCTT CCCAGCTCTC AGCATCTAAT AACAAATGTT CAATCCTGAT GCTAGCAGAC 
ACTGAGAATG AGAAGAATAA GTGGGTGGGA GTGCTGAGTG AATTGCACAA GATTTTGAAG 
AAAAACAAAT TCAGAGACCG CTCAGTCTAT GTTCCCAAAG AGGCTTATGA CAGCACTCTA 
CCCCTCATTA AAACAACCCA GGCAGCCGCA ATCATAGATC ATGAAAGAAT TGCTTTGGGA 
AACGAAGAAG GGTTATTTGT TGTACATGTC ACCAAAGATG AAATTATTAG AGTTGGTGAC 
AATAAGAAGA TTCATCAGAT TGAACTCATT CCAAATGATC AGCTTGTTGC TGTGATCTCA 
GGACGAAATC GTCATGTACG ACTTTTTCCT ATGTCAGCAT TGGATGGGCG AGAGACCGAT 
TTTTACAAGC TGTCAGAAAC TAAAGGGTGT CAAACCGTAA CTTCTGGAAA GGTGCGCCAT 
GGAGCTCTCA CATGCCTGTG TGTGGCTATG AAAAGGCAGG TCCTCTGTTA TGAACTATTT 
CAGAGCAAGA CCCGTCACAG AAAATTTAAA GAAATTCAAG TCCCATATAA TGTCCAGTGG 
ATGGCAATCT TCAGTGAACA ACTCTGTGTG GGATTCCAGT CAGGATTTCT AAGATACCCC 
TTGAATGGAG AAGGAAATCC ATACAGTATG CTCCATTCAA ATGACCATAC ACTATCATTT 
ATTGCACATC AACCAATGGA TGCTATCTGC GCAGTTGAGA TCTCCAGTAA AGAATATCTG 
CTGTGTTTTA ACAGCATTGG GATATACACT GACTGCCAGG GCCGAAGATC TAGACAACAG 
GAATTGATGT GGCCAGCAAA TCCTTCCTCT TGTTGTTACA ATGCACCATA TCTCTCGGTG 
TACAGTGAAA ATGCAGTTGA TATCTTTGAT GTGAACTCCA TGGAATGGAT TCAGACTCTT 
CCTCTCAAAA AGGTTCGACC CTTAAACAAT GAAGGATCAT TAAATCTTTT AGGGTTGGAG 
ACCATTAGAT TAATATATTT CAAAAATAAG ATGGCAGAAG GGGACGAACT GGTAGTACCT 
GAAACATCAG ATAATAGTCG GAAACAAATG GTTAGAAACA TTAACAATAA GCGGCGTTAT 
TCCTTCAGAG TCCCAGAAGA GGAAAGGATG CAGCAGAGGA GGGAAATGCT ACGAGATCCA 
GAAATGAGAA ATAAATTAAT TTCTAATCCA ACTAATTTTA ATCACATAGC ACACATGGGT 
CCTGGAGATG GAATACAGAT CCTGAAAGAT CTGCCCATGC CAGGTTTCCC TTATCCATCC 
CCTCATCATC ACTCCGGTCT GATCTCCTCT CCGATCAACT TTGAACACAT CTATCACATG 
ACTGTTAATT CTGCTGAAAA ATTTCTCTCT CCTGATTCCA TAAACCCTGA ATATTCCCCG 
TCTCTGCGCT CTGTCCCTGG TACACCAAGC TTTATGACTC TGAGGAACCC TCGGCCTCAG 
GAAAGTCGGA CAGTATTCAG TGGCTCAGTC AGTATTCCAT CTATCACCAA ATCCCGCCCT 
GAGCCAGGCC GCTCCATGAG TGCTAGCAGT GGCTTGTCAG CAAGGTCATC CGCACAGAAT 
GGCAGCGCAT TAAAGAGGGA ATTCTCTGGA GGAAGCTACA GTGCCAAGCG GCAGCCCATG 
CCCTCCCCGT CAGAGGGCTC TTTGTCCTCT GGAGGCATGG ACCAAGGAAG TGATGCCCCA 
GCGAGGGACT TTGACGGAGA GGACTCTGAC TCTCCGAGGC ATTCCACAGC TTCCAACAGT 
TCCAACCTAA GCAGCCCCCC AAGCCCAGCT TCACCCCGAA AAACCAAGAG CCTCTCCCTG 
GAGAGCACTG ACCGCGGGAG CTGGGACCCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_011544308.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542610.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X8, mRNA.

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CloneID	OHu61835
Clone ID Related Accession (Same CDS sequence)	XM_011544308.2 , XM_011544308.3
Accession Version	XM_011544308.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011544308.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC ATCCTGTACT CCAGCTAGCA AAGGCAGACG TACTGATCCC 
GTTGAGAACA CATATGTATG GAACCCGAGC GTCAAGTTTC ACATCCAGTC ACGGTCCACA 
TCTCCTTCCA CATCTAGTGA AGCTGAGCCA GTTAAGACTG TAGACTCCAC TCCACTTTCA 
GTTCACACAC CAACCTTAAG GAAAAAAGGA TGTCCTGGTT CAACTGGCTT TCCACCTAAG 
CGCAAGACTC ACCAGTTTTT TGTAAAATCT TTTACTACTC CTACCAAGTG TCATCAGTGT 
ACCTCCTTGA TGGTGGGTTT AATAAGACAG GGCTGTTCAT GTGAAGTGTG TGGATTCTCA 
TGCCATATAA CTTGTGTAAA CAAAGCTCCA ACCACTTGTC CAGTTCCTCC TGAACAGACA 
AAAGGTCCCC TGGGTATAGA TCCTCAGAAA GGAATAGGAA CAGCATATGA AGGTCATGTC 
AGGATTCCTA AGCCAGCTGG AGTGAAGAAA GGGTGGCAGA GAGCACTGGC TATAGTGTGT 
GACTTCAAAC TCTTTCTGTA CGATATTGCT GAAGGAAAAG CATCTCAGCC CAGTGTTGTC 
ATTAGTCAAG TGATTGACAT GAGGGATGAA GAATTTTCTG TGAGTTCAGT CTTGGCTTCT 
GATGTTATCC ATGCAAGTCG GAAAGATATA CCCTGTATAT TTAGGGTCAC AGCTTCCCAG 
CTCTCAGCAT CTAATAACAA ATGTTCAATC CTGATGCTAG CAGACACTGA GAATGAGAAG 
AATAAGTGGG TGGGAGTGCT GAGTGAATTG CACAAGATTT TGAAGAAAAA CAAATTCAGA 
GACCGCTCAG TCTATGTTCC CAAAGAGGCT TATGACAGCA CTCTACCCCT CATTAAAACA 
ACCCAGGCAG CCGCAATCAT AGATCATGAA AGAATTGCTT TGGGAAACGA AGAAGGGTTA 
TTTGTTGTAC ATGTCACCAA AGATGAAATT ATTAGAGTTG GTGACAATAA GAAGATTCAT 
CAGATTGAAC TCATTCCAAA TGATCAGCTT GTTGCTGTGA TCTCAGGACG AAATCGTCAT 
GTACGACTTT TTCCTATGTC AGCATTGGAT GGGCGAGAGA CCGATTTTTA CAAGCTGTCA 
GAAACTAAAG GGTGTCAAAC CGTAACTTCT GGAAAGGTGC GCCATGGAGC TCTCACATGC 
CTGTGTGTGG CTATGAAAAG GCAGGTCCTC TGTTATGAAC TATTTCAGAG CAAGACCCGT 
CACAGAAAAT TTAAAGAAAT TCAAGTCCCA TATAATGTCC AGTGGATGGC AATCTTCAGT 
GAACAACTCT GTGTGGGATT CCAGTCAGGA TTTCTAAGAT ACCCCTTGAA TGGAGAAGGA 
AATCCATACA GTATGCTCCA TTCAAATGAC CATACACTAT CATTTATTGC ACATCAACCA 
ATGGATGCTA TCTGCGCAGT TGAGATCTCC AGTAAAGAAT ATCTGCTGTG TTTTAACAGC 
ATTGGGATAT ACACTGACTG CCAGGGCCGA AGATCTAGAC AACAGGAATT GATGTGGCCA 
GCAAATCCTT CCTCTTGTTG TTACAATGCA CCATATCTCT CGGTGTACAG TGAAAATGCA 
GTTGATATCT TTGATGTGAA CTCCATGGAA TGGATTCAGA CTCTTCCTCT CAAAAAGGTT 
CGACCCTTAA ACAATGAAGG ATCATTAAAT CTTTTAGGGT TGGAGACCAT TAGATTAATA 
TATTTCAAAA ATAAGATGGC AGAAGGGGAC GAACTGGTAG TACCTGAAAC ATCAGATAAT 
AGTCGGAAAC AAATGGTTAG AAACATTAAC AATAAGCGGC GTTATTCCTT CAGAGTCCCA 
GAAGAGGAAA GGATGCAGCA GAGGAGGGAA ATGCTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ATCCAACTAA TTTTAATCAC ATAGCACACA TGGGTCCTGG AGATGGAATA 
CAGATCCTGA AAGATCTGCC CATGAACCCT CGGCCTCAGG AAAGTCGGAC AGTATTCAGT 
GGCTCAGTCA GTATTCCATC TATCACCAAA TCCCGCCCTG AGCCAGGCCG CTCCATGAGT 
GCTAGCAGTG GCTTGTCAGC AAGGTCATCC GCACAGAATG GCAGCGCATT AAAGAGGGAA 
TTCTCTGGAG GAAGCTACAG TGCCAAGCGG CAGCCCATGC CCTCCCCGTC AGAGGGCTCT 
TTGTCCTCTG GAGGCATGGA CCAAGGAAGT GATGCCCCAG CGAGGGACTT TGACGGAGAG 
GACTCTGACT CTCCGAGGCA TTCCACAGCT TCCAACAGTT CCAACCTAAG CAGCCCCCCA 
AGCCCAGCTT CACCCCGAAA AACCAAGAGC CTCTCCCTGG AGAGCACTGA CCGCGGGAGC 
TGGGACCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542610.1
CDS	1056..6407
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVENTYVWNPSVKFHIQSRSTSPSTSSEAEPVKTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_011544308.2
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544308.2

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC ATCCTGTACT CCAGCTAGCA AAGGCAGACG TACTGATCCC 
GTTGAGAACA CATATGTATG GAACCCGAGC GTCAAGTTTC ACATCCAGTC ACGGTCCACA 
TCTCCTTCCA CATCTAGTGA AGCTGAGCCA GTTAAGACTG TAGACTCCAC TCCACTTTCA 
GTTCACACAC CAACCTTAAG GAAAAAAGGA TGTCCTGGTT CAACTGGCTT TCCACCTAAG 
CGCAAGACTC ACCAGTTTTT TGTAAAATCT TTTACTACTC CTACCAAGTG TCATCAGTGT 
ACCTCCTTGA TGGTGGGTTT AATAAGACAG GGCTGTTCAT GTGAAGTGTG TGGATTCTCA 
TGCCATATAA CTTGTGTAAA CAAAGCTCCA ACCACTTGTC CAGTTCCTCC TGAACAGACA 
AAAGGTCCCC TGGGTATAGA TCCTCAGAAA GGAATAGGAA CAGCATATGA AGGTCATGTC 
AGGATTCCTA AGCCAGCTGG AGTGAAGAAA GGGTGGCAGA GAGCACTGGC TATAGTGTGT 
GACTTCAAAC TCTTTCTGTA CGATATTGCT GAAGGAAAAG CATCTCAGCC CAGTGTTGTC 
ATTAGTCAAG TGATTGACAT GAGGGATGAA GAATTTTCTG TGAGTTCAGT CTTGGCTTCT 
GATGTTATCC ATGCAAGTCG GAAAGATATA CCCTGTATAT TTAGGGTCAC AGCTTCCCAG 
CTCTCAGCAT CTAATAACAA ATGTTCAATC CTGATGCTAG CAGACACTGA GAATGAGAAG 
AATAAGTGGG TGGGAGTGCT GAGTGAATTG CACAAGATTT TGAAGAAAAA CAAATTCAGA 
GACCGCTCAG TCTATGTTCC CAAAGAGGCT TATGACAGCA CTCTACCCCT CATTAAAACA 
ACCCAGGCAG CCGCAATCAT AGATCATGAA AGAATTGCTT TGGGAAACGA AGAAGGGTTA 
TTTGTTGTAC ATGTCACCAA AGATGAAATT ATTAGAGTTG GTGACAATAA GAAGATTCAT 
CAGATTGAAC TCATTCCAAA TGATCAGCTT GTTGCTGTGA TCTCAGGACG AAATCGTCAT 
GTACGACTTT TTCCTATGTC AGCATTGGAT GGGCGAGAGA CCGATTTTTA CAAGCTGTCA 
GAAACTAAAG GGTGTCAAAC CGTAACTTCT GGAAAGGTGC GCCATGGAGC TCTCACATGC 
CTGTGTGTGG CTATGAAAAG GCAGGTCCTC TGTTATGAAC TATTTCAGAG CAAGACCCGT 
CACAGAAAAT TTAAAGAAAT TCAAGTCCCA TATAATGTCC AGTGGATGGC AATCTTCAGT 
GAACAACTCT GTGTGGGATT CCAGTCAGGA TTTCTAAGAT ACCCCTTGAA TGGAGAAGGA 
AATCCATACA GTATGCTCCA TTCAAATGAC CATACACTAT CATTTATTGC ACATCAACCA 
ATGGATGCTA TCTGCGCAGT TGAGATCTCC AGTAAAGAAT ATCTGCTGTG TTTTAACAGC 
ATTGGGATAT ACACTGACTG CCAGGGCCGA AGATCTAGAC AACAGGAATT GATGTGGCCA 
GCAAATCCTT CCTCTTGTTG TTACAATGCA CCATATCTCT CGGTGTACAG TGAAAATGCA 
GTTGATATCT TTGATGTGAA CTCCATGGAA TGGATTCAGA CTCTTCCTCT CAAAAAGGTT 
CGACCCTTAA ACAATGAAGG ATCATTAAAT CTTTTAGGGT TGGAGACCAT TAGATTAATA 
TATTTCAAAA ATAAGATGGC AGAAGGGGAC GAACTGGTAG TACCTGAAAC ATCAGATAAT 
AGTCGGAAAC AAATGGTTAG AAACATTAAC AATAAGCGGC GTTATTCCTT CAGAGTCCCA 
GAAGAGGAAA GGATGCAGCA GAGGAGGGAA ATGCTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ATCCAACTAA TTTTAATCAC ATAGCACACA TGGGTCCTGG AGATGGAATA 
CAGATCCTGA AAGATCTGCC CATGAACCCT CGGCCTCAGG AAAGTCGGAC AGTATTCAGT 
GGCTCAGTCA GTATTCCATC TATCACCAAA TCCCGCCCTG AGCCAGGCCG CTCCATGAGT 
GCTAGCAGTG GCTTGTCAGC AAGGTCATCC GCACAGAATG GCAGCGCATT AAAGAGGGAA 
TTCTCTGGAG GAAGCTACAG TGCCAAGCGG CAGCCCATGC CCTCCCCGTC AGAGGGCTCT 
TTGTCCTCTG GAGGCATGGA CCAAGGAAGT GATGCCCCAG CGAGGGACTT TGACGGAGAG 
GACTCTGACT CTCCGAGGCA TTCCACAGCT TCCAACAGTT CCAACCTAAG CAGCCCCCCA 
AGCCCAGCTT CACCCCGAAA AACCAAGAGC CTCTCCCTGG AGAGCACTGA CCGCGGGAGC 
TGGGACCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_011544308.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542610.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X8, mRNA.

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CloneID	OHu61835
Clone ID Related Accession (Same CDS sequence)	XM_011544308.2 , XM_011544308.3
Accession Version	XM_011544308.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011544308.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC ATCCTGTACT CCAGCTAGCA AAGGCAGACG TACTGATCCC 
GTTGAGAACA CATATGTATG GAACCCGAGC GTCAAGTTTC ACATCCAGTC ACGGTCCACA 
TCTCCTTCCA CATCTAGTGA AGCTGAGCCA GTTAAGACTG TAGACTCCAC TCCACTTTCA 
GTTCACACAC CAACCTTAAG GAAAAAAGGA TGTCCTGGTT CAACTGGCTT TCCACCTAAG 
CGCAAGACTC ACCAGTTTTT TGTAAAATCT TTTACTACTC CTACCAAGTG TCATCAGTGT 
ACCTCCTTGA TGGTGGGTTT AATAAGACAG GGCTGTTCAT GTGAAGTGTG TGGATTCTCA 
TGCCATATAA CTTGTGTAAA CAAAGCTCCA ACCACTTGTC CAGTTCCTCC TGAACAGACA 
AAAGGTCCCC TGGGTATAGA TCCTCAGAAA GGAATAGGAA CAGCATATGA AGGTCATGTC 
AGGATTCCTA AGCCAGCTGG AGTGAAGAAA GGGTGGCAGA GAGCACTGGC TATAGTGTGT 
GACTTCAAAC TCTTTCTGTA CGATATTGCT GAAGGAAAAG CATCTCAGCC CAGTGTTGTC 
ATTAGTCAAG TGATTGACAT GAGGGATGAA GAATTTTCTG TGAGTTCAGT CTTGGCTTCT 
GATGTTATCC ATGCAAGTCG GAAAGATATA CCCTGTATAT TTAGGGTCAC AGCTTCCCAG 
CTCTCAGCAT CTAATAACAA ATGTTCAATC CTGATGCTAG CAGACACTGA GAATGAGAAG 
AATAAGTGGG TGGGAGTGCT GAGTGAATTG CACAAGATTT TGAAGAAAAA CAAATTCAGA 
GACCGCTCAG TCTATGTTCC CAAAGAGGCT TATGACAGCA CTCTACCCCT CATTAAAACA 
ACCCAGGCAG CCGCAATCAT AGATCATGAA AGAATTGCTT TGGGAAACGA AGAAGGGTTA 
TTTGTTGTAC ATGTCACCAA AGATGAAATT ATTAGAGTTG GTGACAATAA GAAGATTCAT 
CAGATTGAAC TCATTCCAAA TGATCAGCTT GTTGCTGTGA TCTCAGGACG AAATCGTCAT 
GTACGACTTT TTCCTATGTC AGCATTGGAT GGGCGAGAGA CCGATTTTTA CAAGCTGTCA 
GAAACTAAAG GGTGTCAAAC CGTAACTTCT GGAAAGGTGC GCCATGGAGC TCTCACATGC 
CTGTGTGTGG CTATGAAAAG GCAGGTCCTC TGTTATGAAC TATTTCAGAG CAAGACCCGT 
CACAGAAAAT TTAAAGAAAT TCAAGTCCCA TATAATGTCC AGTGGATGGC AATCTTCAGT 
GAACAACTCT GTGTGGGATT CCAGTCAGGA TTTCTAAGAT ACCCCTTGAA TGGAGAAGGA 
AATCCATACA GTATGCTCCA TTCAAATGAC CATACACTAT CATTTATTGC ACATCAACCA 
ATGGATGCTA TCTGCGCAGT TGAGATCTCC AGTAAAGAAT ATCTGCTGTG TTTTAACAGC 
ATTGGGATAT ACACTGACTG CCAGGGCCGA AGATCTAGAC AACAGGAATT GATGTGGCCA 
GCAAATCCTT CCTCTTGTTG TTACAATGCA CCATATCTCT CGGTGTACAG TGAAAATGCA 
GTTGATATCT TTGATGTGAA CTCCATGGAA TGGATTCAGA CTCTTCCTCT CAAAAAGGTT 
CGACCCTTAA ACAATGAAGG ATCATTAAAT CTTTTAGGGT TGGAGACCAT TAGATTAATA 
TATTTCAAAA ATAAGATGGC AGAAGGGGAC GAACTGGTAG TACCTGAAAC ATCAGATAAT 
AGTCGGAAAC AAATGGTTAG AAACATTAAC AATAAGCGGC GTTATTCCTT CAGAGTCCCA 
GAAGAGGAAA GGATGCAGCA GAGGAGGGAA ATGCTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ATCCAACTAA TTTTAATCAC ATAGCACACA TGGGTCCTGG AGATGGAATA 
CAGATCCTGA AAGATCTGCC CATGAACCCT CGGCCTCAGG AAAGTCGGAC AGTATTCAGT 
GGCTCAGTCA GTATTCCATC TATCACCAAA TCCCGCCCTG AGCCAGGCCG CTCCATGAGT 
GCTAGCAGTG GCTTGTCAGC AAGGTCATCC GCACAGAATG GCAGCGCATT AAAGAGGGAA 
TTCTCTGGAG GAAGCTACAG TGCCAAGCGG CAGCCCATGC CCTCCCCGTC AGAGGGCTCT 
TTGTCCTCTG GAGGCATGGA CCAAGGAAGT GATGCCCCAG CGAGGGACTT TGACGGAGAG 
GACTCTGACT CTCCGAGGCA TTCCACAGCT TCCAACAGTT CCAACCTAAG CAGCCCCCCA 
AGCCCAGCTT CACCCCGAAA AACCAAGAGC CTCTCCCTGG AGAGCACTGA CCGCGGGAGC 
TGGGACCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542610.1
CDS	969..6320
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVENTYVWNPSVKFHIQSRSTSPSTSSEAEPVKTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_011544308.3
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544308.3

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TCTAGCTGCT 
GAAGCATCTA AAGACAGGAA GCTACGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGAGT ATGCAGCATA 
GAACATCAGC AAGAGATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TATCTAAAAG AGAAGGAATA CATGCAAATG AAATAAAAAA TCTTAAGAAA 
GAACTGCATG ATTCAGAAGG TCAGCAACTT GCTCTCAACA AAGAAATTAT GATTTTAAAA 
GACAAATTGG AAAAAACCAG AAGAGAAAGT CAAAGTGAAA GGGAGGAATT TGAAAGTGAG 
TTCAAACAAC AATATGAACG AGAAAAAGTG TTGTTAACTG AAGAAAATAA AAAGCTGACG 
AGTGAACTTG ATAAGCTTAC TACTTTGTAT GAGAACTTAA GTATACACAA CCAGCAGTTA 
GAAGAAGAGG TTAAAGATCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGCGAT GAAAAGGATG CACGAGGGTA TCTTCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAG GCATTAAGAA ATTCCAGCTT GGGTACACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCGAAAC TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CGGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGTTG 
AATAAAGTTA AAGCATCTAA TATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTCTCAGA AATCGAACAG CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC ATCCTGTACT CCAGCTAGCA AAGGCAGACG TACTGATCCC 
GTTGAGAACA CATATGTATG GAACCCGAGC GTCAAGTTTC ACATCCAGTC ACGGTCCACA 
TCTCCTTCCA CATCTAGTGA AGCTGAGCCA GTTAAGACTG TAGACTCCAC TCCACTTTCA 
GTTCACACAC CAACCTTAAG GAAAAAAGGA TGTCCTGGTT CAACTGGCTT TCCACCTAAG 
CGCAAGACTC ACCAGTTTTT TGTAAAATCT TTTACTACTC CTACCAAGTG TCATCAGTGT 
ACCTCCTTGA TGGTGGGTTT AATAAGACAG GGCTGTTCAT GTGAAGTGTG TGGATTCTCA 
TGCCATATAA CTTGTGTAAA CAAAGCTCCA ACCACTTGTC CAGTTCCTCC TGAACAGACA 
AAAGGTCCCC TGGGTATAGA TCCTCAGAAA GGAATAGGAA CAGCATATGA AGGTCATGTC 
AGGATTCCTA AGCCAGCTGG AGTGAAGAAA GGGTGGCAGA GAGCACTGGC TATAGTGTGT 
GACTTCAAAC TCTTTCTGTA CGATATTGCT GAAGGAAAAG CATCTCAGCC CAGTGTTGTC 
ATTAGTCAAG TGATTGACAT GAGGGATGAA GAATTTTCTG TGAGTTCAGT CTTGGCTTCT 
GATGTTATCC ATGCAAGTCG GAAAGATATA CCCTGTATAT TTAGGGTCAC AGCTTCCCAG 
CTCTCAGCAT CTAATAACAA ATGTTCAATC CTGATGCTAG CAGACACTGA GAATGAGAAG 
AATAAGTGGG TGGGAGTGCT GAGTGAATTG CACAAGATTT TGAAGAAAAA CAAATTCAGA 
GACCGCTCAG TCTATGTTCC CAAAGAGGCT TATGACAGCA CTCTACCCCT CATTAAAACA 
ACCCAGGCAG CCGCAATCAT AGATCATGAA AGAATTGCTT TGGGAAACGA AGAAGGGTTA 
TTTGTTGTAC ATGTCACCAA AGATGAAATT ATTAGAGTTG GTGACAATAA GAAGATTCAT 
CAGATTGAAC TCATTCCAAA TGATCAGCTT GTTGCTGTGA TCTCAGGACG AAATCGTCAT 
GTACGACTTT TTCCTATGTC AGCATTGGAT GGGCGAGAGA CCGATTTTTA CAAGCTGTCA 
GAAACTAAAG GGTGTCAAAC CGTAACTTCT GGAAAGGTGC GCCATGGAGC TCTCACATGC 
CTGTGTGTGG CTATGAAAAG GCAGGTCCTC TGTTATGAAC TATTTCAGAG CAAGACCCGT 
CACAGAAAAT TTAAAGAAAT TCAAGTCCCA TATAATGTCC AGTGGATGGC AATCTTCAGT 
GAACAACTCT GTGTGGGATT CCAGTCAGGA TTTCTAAGAT ACCCCTTGAA TGGAGAAGGA 
AATCCATACA GTATGCTCCA TTCAAATGAC CATACACTAT CATTTATTGC ACATCAACCA 
ATGGATGCTA TCTGCGCAGT TGAGATCTCC AGTAAAGAAT ATCTGCTGTG TTTTAACAGC 
ATTGGGATAT ACACTGACTG CCAGGGCCGA AGATCTAGAC AACAGGAATT GATGTGGCCA 
GCAAATCCTT CCTCTTGTTG TTACAATGCA CCATATCTCT CGGTGTACAG TGAAAATGCA 
GTTGATATCT TTGATGTGAA CTCCATGGAA TGGATTCAGA CTCTTCCTCT CAAAAAGGTT 
CGACCCTTAA ACAATGAAGG ATCATTAAAT CTTTTAGGGT TGGAGACCAT TAGATTAATA 
TATTTCAAAA ATAAGATGGC AGAAGGGGAC GAACTGGTAG TACCTGAAAC ATCAGATAAT 
AGTCGGAAAC AAATGGTTAG AAACATTAAC AATAAGCGGC GTTATTCCTT CAGAGTCCCA 
GAAGAGGAAA GGATGCAGCA GAGGAGGGAA ATGCTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ATCCAACTAA TTTTAATCAC ATAGCACACA TGGGTCCTGG AGATGGAATA 
CAGATCCTGA AAGATCTGCC CATGAACCCT CGGCCTCAGG AAAGTCGGAC AGTATTCAGT 
GGCTCAGTCA GTATTCCATC TATCACCAAA TCCCGCCCTG AGCCAGGCCG CTCCATGAGT 
GCTAGCAGTG GCTTGTCAGC AAGGTCATCC GCACAGAATG GCAGCGCATT AAAGAGGGAA 
TTCTCTGGAG GAAGCTACAG TGCCAAGCGG CAGCCCATGC CCTCCCCGTC AGAGGGCTCT 
TTGTCCTCTG GAGGCATGGA CCAAGGAAGT GATGCCCCAG CGAGGGACTT TGACGGAGAG 
GACTCTGACT CTCCGAGGCA TTCCACAGCT TCCAACAGTT CCAACCTAAG CAGCCCCCCA 
AGCCCAGCTT CACCCCGAAA AACCAAGAGC CTCTCCCTGG AGAGCACTGA CCGCGGGAGC 
TGGGACCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_011544309.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542611.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X11, mRNA.

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CloneID	OHu61836
Clone ID Related Accession (Same CDS sequence)	XM_011544309.2 , XM_011544309.3
Accession Version	XM_011544309.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011544309.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGCTG GAAGTTCATA CAGAAGCTCT AGCTGCTGAA 
GCATCTAAAG ACAGGAAGCT ACGTGAACAG AGTGAGCACT ATTCTAAGCA ACTGGAAAAT 
GAATTGGAGG GACTGAAGCA AAAACAAATT AGTTACTCAC CAGGAGTATG CAGCATAGAA 
CATCAGCAAG AGATAACCAA ACTAAAGACT GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAATTAT CTAAAAGAGA AGGAATACAT GCAAATGAAA TAAAAAATCT TAAGAAAGAA 
CTGCATGATT CAGAAGGTCA GCAACTTGCT CTCAACAAAG AAATTATGAT TTTAAAAGAC 
AAATTGGAAA AAACCAGAAG AGAAAGTCAA AGTGAAAGGG AGGAATTTGA AAGTGAGTTC 
AAACAACAAT ATGAACGAGA AAAAGTGTTG TTAACTGAAG AAAATAAAAA GCTGACGAGT 
GAACTTGATA AGCTTACTAC TTTGTATGAG AACTTAAGTA TACACAACCA GCAGTTAGAA 
GAAGAGGTTA AAGATCTAGC AGACAAGAAA GAATCAGTTG CACATTGGGA AGCCCAAATC 
ACAGAAATAA TTCAGTGGGT CAGCGATGAA AAGGATGCAC GAGGGTATCT TCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAGGCA TTAAGAAATT CCAGCTTGGG TACACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCGAAACTGG ATATGTCAGC TAGACTGGAG 
TTGCAGTCGG CTCTGGATGC AGAAATAAGA GCCAAACAGG CCATCCAAGA AGAGTTGAAT 
AAAGTTAAAG CATCTAATAT CATAACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAACTAC TCTCAGAAAT CGAACAGCTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AGATTCCTTT TCTTCTTCCT CATCTTCACT GATTGATTTT 
TTGGATGACC GCTCTCCATC CTGTACTCCA GCTAGCAAAG GCAGACGTAC TGATCCCGTT 
GAGAACACAT ATGTATGGAA CCCGAGCGTC AAGTTTCACA TCCAGTCACG GTCCACATCT 
CCTTCCACAT CTAGTGAAGC TGAGCCAGTT AAGACTGTAG ACTCCACTCC ACTTTCAGTT 
CACACACCAA CCTTAAGGAA AAAAGGATGT CCTGGTTCAA CTGGCTTTCC ACCTAAGCGC 
AAGACTCACC AGTTTTTTGT AAAATCTTTT ACTACTCCTA CCAAGTGTCA TCAGTGTACC 
TCCTTGATGG TGGGTTTAAT AAGACAGGGC TGTTCATGTG AAGTGTGTGG ATTCTCATGC 
CATATAACTT GTGTAAACAA AGCTCCAACC ACTTGTCCAG TTCCTCCTGA ACAGACAAAA 
GGTCCCCTGG GTATAGATCC TCAGAAAGGA ATAGGAACAG CATATGAAGG TCATGTCAGG 
ATTCCTAAGC CAGCTGGAGT GAAGAAAGGG TGGCAGAGAG CACTGGCTAT AGTGTGTGAC 
TTCAAACTCT TTCTGTACGA TATTGCTGAA GGAAAAGCAT CTCAGCCCAG TGTTGTCATT 
AGTCAAGTGA TTGACATGAG GGATGAAGAA TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT 
GTTATCCATG CAAGTCGGAA AGATATACCC TGTATATTTA GGGTCACAGC TTCCCAGCTC 
TCAGCATCTA ATAACAAATG TTCAATCCTG ATGCTAGCAG ACACTGAGAA TGAGAAGAAT 
AAGTGGGTGG GAGTGCTGAG TGAATTGCAC AAGATTTTGA AGAAAAACAA ATTCAGAGAC 
CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT GACAGCACTC TACCCCTCAT TAAAACAACC 
CAGGCAGCCG CAATCATAGA TCATGAAAGA ATTGCTTTGG GAAACGAAGA AGGGTTATTT 
GTTGTACATG TCACCAAAGA TGAAATTATT AGAGTTGGTG ACAATAAGAA GATTCATCAG 
ATTGAACTCA TTCCAAATGA TCAGCTTGTT GCTGTGATCT CAGGACGAAA TCGTCATGTA 
CGACTTTTTC CTATGTCAGC ATTGGATGGG CGAGAGACCG ATTTTTACAA GCTGTCAGAA 
ACTAAAGGGT GTCAAACCGT AACTTCTGGA AAGGTGCGCC ATGGAGCTCT CACATGCCTG 
TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT TATGAACTAT TTCAGAGCAA GACCCGTCAC 
AGAAAATTTA AAGAAATTCA AGTCCCATAT AATGTCCAGT GGATGGCAAT CTTCAGTGAA 
CAACTCTGTG TGGGATTCCA GTCAGGATTT CTAAGATACC CCTTGAATGG AGAAGGAAAT 
CCATACAGTA TGCTCCATTC AAATGACCAT ACACTATCAT TTATTGCACA TCAACCAATG 
GATGCTATCT GCGCAGTTGA GATCTCCAGT AAAGAATATC TGCTGTGTTT TAACAGCATT 
GGGATATACA CTGACTGCCA GGGCCGAAGA TCTAGACAAC AGGAATTGAT GTGGCCAGCA 
AATCCTTCCT CTTGTTGTTA CAATGCACCA TATCTCTCGG TGTACAGTGA AAATGCAGTT 
GATATCTTTG ATGTGAACTC CATGGAATGG ATTCAGACTC TTCCTCTCAA AAAGGTTCGA 
CCCTTAAACA ATGAAGGATC ATTAAATCTT TTAGGGTTGG AGACCATTAG ATTAATATAT 
TTCAAAAATA AGATGGCAGA AGGGGACGAA CTGGTAGTAC CTGAAACATC AGATAATAGT 
CGGAAACAAA TGGTTAGAAA CATTAACAAT AAGCGGCGTT ATTCCTTCAG AGTCCCAGAA 
GAGGAAAGGA TGCAGCAGAG GAGGGAAATG CTACGAGATC CAGAAATGAG AAATAAATTA 
ATTTCTAATC CAACTAATTT TAATCACATA GCACACATGG GTCCTGGAGA TGGAATACAG 
ATCCTGAAAG ATCTGCCCAT GCCAGGTTTC CCTTATCCAT CCCCTCATCA TCACTCCGGT 
CTGATCTCCT CTCCGATCAA CTTTGAACAC ATCTATCACA TGACTGTTAA TTCTGCTGAA 
AAATTTCTCT CTCCTGATTC CATAAACCCT GAATATTCCC CGTCTCTGCG CTCTGTCCCT 
GGTACACCAA GCTTTATGAC TCTGAGGAAC CCTCGGCCTC AGGAAAGTCG GACAGTATTC 
AGTGGCTCAG TCAGTATTCC ATCTATCACC AAATCCCGCC CTGAGCCAGG CCGCTCCATG 
AGTGCTAGCA GTGGCTTGTC AGCAAGGTCA TCCGCACAGA ATGGCAGCGC ATTAAAGAGG 
GAATTCTCTG GAGGAAGCTA CAGTGCCAAG CGGCAGCCCA TGCCCTCCCC GTCAGAGGGC 
TCTTTGTCCT CTGGAGGCAT GGACCAAGGA AGTGATGCCC CAGCGAGGGA CTTTGACGGA 
GAGGACTCTG ACTCTCCGAG GCATTCCACA GCTTCCAACA GTTCCAACCT AAGCAGCCCC 
CCAAGCCCAG CTTCACCCCG AAAAACCAAG AGCCTCTCCC TGGAGAGCAC TGACCGCGGG 
AGCTGGGACC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542611.1
CDS	1056..6350
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKLEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVENTYVWNPSVKFHIQSRSTSPSTSSEAEPVKTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_011544309.2
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544309.2

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGCTG GAAGTTCATA CAGAAGCTCT AGCTGCTGAA 
GCATCTAAAG ACAGGAAGCT ACGTGAACAG AGTGAGCACT ATTCTAAGCA ACTGGAAAAT 
GAATTGGAGG GACTGAAGCA AAAACAAATT AGTTACTCAC CAGGAGTATG CAGCATAGAA 
CATCAGCAAG AGATAACCAA ACTAAAGACT GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAATTAT CTAAAAGAGA AGGAATACAT GCAAATGAAA TAAAAAATCT TAAGAAAGAA 
CTGCATGATT CAGAAGGTCA GCAACTTGCT CTCAACAAAG AAATTATGAT TTTAAAAGAC 
AAATTGGAAA AAACCAGAAG AGAAAGTCAA AGTGAAAGGG AGGAATTTGA AAGTGAGTTC 
AAACAACAAT ATGAACGAGA AAAAGTGTTG TTAACTGAAG AAAATAAAAA GCTGACGAGT 
GAACTTGATA AGCTTACTAC TTTGTATGAG AACTTAAGTA TACACAACCA GCAGTTAGAA 
GAAGAGGTTA AAGATCTAGC AGACAAGAAA GAATCAGTTG CACATTGGGA AGCCCAAATC 
ACAGAAATAA TTCAGTGGGT CAGCGATGAA AAGGATGCAC GAGGGTATCT TCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAGGCA TTAAGAAATT CCAGCTTGGG TACACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCGAAACTGG ATATGTCAGC TAGACTGGAG 
TTGCAGTCGG CTCTGGATGC AGAAATAAGA GCCAAACAGG CCATCCAAGA AGAGTTGAAT 
AAAGTTAAAG CATCTAATAT CATAACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAACTAC TCTCAGAAAT CGAACAGCTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AGATTCCTTT TCTTCTTCCT CATCTTCACT GATTGATTTT 
TTGGATGACC GCTCTCCATC CTGTACTCCA GCTAGCAAAG GCAGACGTAC TGATCCCGTT 
GAGAACACAT ATGTATGGAA CCCGAGCGTC AAGTTTCACA TCCAGTCACG GTCCACATCT 
CCTTCCACAT CTAGTGAAGC TGAGCCAGTT AAGACTGTAG ACTCCACTCC ACTTTCAGTT 
CACACACCAA CCTTAAGGAA AAAAGGATGT CCTGGTTCAA CTGGCTTTCC ACCTAAGCGC 
AAGACTCACC AGTTTTTTGT AAAATCTTTT ACTACTCCTA CCAAGTGTCA TCAGTGTACC 
TCCTTGATGG TGGGTTTAAT AAGACAGGGC TGTTCATGTG AAGTGTGTGG ATTCTCATGC 
CATATAACTT GTGTAAACAA AGCTCCAACC ACTTGTCCAG TTCCTCCTGA ACAGACAAAA 
GGTCCCCTGG GTATAGATCC TCAGAAAGGA ATAGGAACAG CATATGAAGG TCATGTCAGG 
ATTCCTAAGC CAGCTGGAGT GAAGAAAGGG TGGCAGAGAG CACTGGCTAT AGTGTGTGAC 
TTCAAACTCT TTCTGTACGA TATTGCTGAA GGAAAAGCAT CTCAGCCCAG TGTTGTCATT 
AGTCAAGTGA TTGACATGAG GGATGAAGAA TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT 
GTTATCCATG CAAGTCGGAA AGATATACCC TGTATATTTA GGGTCACAGC TTCCCAGCTC 
TCAGCATCTA ATAACAAATG TTCAATCCTG ATGCTAGCAG ACACTGAGAA TGAGAAGAAT 
AAGTGGGTGG GAGTGCTGAG TGAATTGCAC AAGATTTTGA AGAAAAACAA ATTCAGAGAC 
CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT GACAGCACTC TACCCCTCAT TAAAACAACC 
CAGGCAGCCG CAATCATAGA TCATGAAAGA ATTGCTTTGG GAAACGAAGA AGGGTTATTT 
GTTGTACATG TCACCAAAGA TGAAATTATT AGAGTTGGTG ACAATAAGAA GATTCATCAG 
ATTGAACTCA TTCCAAATGA TCAGCTTGTT GCTGTGATCT CAGGACGAAA TCGTCATGTA 
CGACTTTTTC CTATGTCAGC ATTGGATGGG CGAGAGACCG ATTTTTACAA GCTGTCAGAA 
ACTAAAGGGT GTCAAACCGT AACTTCTGGA AAGGTGCGCC ATGGAGCTCT CACATGCCTG 
TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT TATGAACTAT TTCAGAGCAA GACCCGTCAC 
AGAAAATTTA AAGAAATTCA AGTCCCATAT AATGTCCAGT GGATGGCAAT CTTCAGTGAA 
CAACTCTGTG TGGGATTCCA GTCAGGATTT CTAAGATACC CCTTGAATGG AGAAGGAAAT 
CCATACAGTA TGCTCCATTC AAATGACCAT ACACTATCAT TTATTGCACA TCAACCAATG 
GATGCTATCT GCGCAGTTGA GATCTCCAGT AAAGAATATC TGCTGTGTTT TAACAGCATT 
GGGATATACA CTGACTGCCA GGGCCGAAGA TCTAGACAAC AGGAATTGAT GTGGCCAGCA 
AATCCTTCCT CTTGTTGTTA CAATGCACCA TATCTCTCGG TGTACAGTGA AAATGCAGTT 
GATATCTTTG ATGTGAACTC CATGGAATGG ATTCAGACTC TTCCTCTCAA AAAGGTTCGA 
CCCTTAAACA ATGAAGGATC ATTAAATCTT TTAGGGTTGG AGACCATTAG ATTAATATAT 
TTCAAAAATA AGATGGCAGA AGGGGACGAA CTGGTAGTAC CTGAAACATC AGATAATAGT 
CGGAAACAAA TGGTTAGAAA CATTAACAAT AAGCGGCGTT ATTCCTTCAG AGTCCCAGAA 
GAGGAAAGGA TGCAGCAGAG GAGGGAAATG CTACGAGATC CAGAAATGAG AAATAAATTA 
ATTTCTAATC CAACTAATTT TAATCACATA GCACACATGG GTCCTGGAGA TGGAATACAG 
ATCCTGAAAG ATCTGCCCAT GCCAGGTTTC CCTTATCCAT CCCCTCATCA TCACTCCGGT 
CTGATCTCCT CTCCGATCAA CTTTGAACAC ATCTATCACA TGACTGTTAA TTCTGCTGAA 
AAATTTCTCT CTCCTGATTC CATAAACCCT GAATATTCCC CGTCTCTGCG CTCTGTCCCT 
GGTACACCAA GCTTTATGAC TCTGAGGAAC CCTCGGCCTC AGGAAAGTCG GACAGTATTC 
AGTGGCTCAG TCAGTATTCC ATCTATCACC AAATCCCGCC CTGAGCCAGG CCGCTCCATG 
AGTGCTAGCA GTGGCTTGTC AGCAAGGTCA TCCGCACAGA ATGGCAGCGC ATTAAAGAGG 
GAATTCTCTG GAGGAAGCTA CAGTGCCAAG CGGCAGCCCA TGCCCTCCCC GTCAGAGGGC 
TCTTTGTCCT CTGGAGGCAT GGACCAAGGA AGTGATGCCC CAGCGAGGGA CTTTGACGGA 
GAGGACTCTG ACTCTCCGAG GCATTCCACA GCTTCCAACA GTTCCAACCT AAGCAGCCCC 
CCAAGCCCAG CTTCACCCCG AAAAACCAAG AGCCTCTCCC TGGAGAGCAC TGACCGCGGG 
AGCTGGGACC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_011544309.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542611.1 Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X11, mRNA.

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CloneID	OHu61836
Clone ID Related Accession (Same CDS sequence)	XM_011544309.2 , XM_011544309.3
Accession Version	XM_011544309.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase MRCK alpha isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011544309.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGCTG GAAGTTCATA CAGAAGCTCT AGCTGCTGAA 
GCATCTAAAG ACAGGAAGCT ACGTGAACAG AGTGAGCACT ATTCTAAGCA ACTGGAAAAT 
GAATTGGAGG GACTGAAGCA AAAACAAATT AGTTACTCAC CAGGAGTATG CAGCATAGAA 
CATCAGCAAG AGATAACCAA ACTAAAGACT GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAATTAT CTAAAAGAGA AGGAATACAT GCAAATGAAA TAAAAAATCT TAAGAAAGAA 
CTGCATGATT CAGAAGGTCA GCAACTTGCT CTCAACAAAG AAATTATGAT TTTAAAAGAC 
AAATTGGAAA AAACCAGAAG AGAAAGTCAA AGTGAAAGGG AGGAATTTGA AAGTGAGTTC 
AAACAACAAT ATGAACGAGA AAAAGTGTTG TTAACTGAAG AAAATAAAAA GCTGACGAGT 
GAACTTGATA AGCTTACTAC TTTGTATGAG AACTTAAGTA TACACAACCA GCAGTTAGAA 
GAAGAGGTTA AAGATCTAGC AGACAAGAAA GAATCAGTTG CACATTGGGA AGCCCAAATC 
ACAGAAATAA TTCAGTGGGT CAGCGATGAA AAGGATGCAC GAGGGTATCT TCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAGGCA TTAAGAAATT CCAGCTTGGG TACACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCGAAACTGG ATATGTCAGC TAGACTGGAG 
TTGCAGTCGG CTCTGGATGC AGAAATAAGA GCCAAACAGG CCATCCAAGA AGAGTTGAAT 
AAAGTTAAAG CATCTAATAT CATAACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAACTAC TCTCAGAAAT CGAACAGCTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AGATTCCTTT TCTTCTTCCT CATCTTCACT GATTGATTTT 
TTGGATGACC GCTCTCCATC CTGTACTCCA GCTAGCAAAG GCAGACGTAC TGATCCCGTT 
GAGAACACAT ATGTATGGAA CCCGAGCGTC AAGTTTCACA TCCAGTCACG GTCCACATCT 
CCTTCCACAT CTAGTGAAGC TGAGCCAGTT AAGACTGTAG ACTCCACTCC ACTTTCAGTT 
CACACACCAA CCTTAAGGAA AAAAGGATGT CCTGGTTCAA CTGGCTTTCC ACCTAAGCGC 
AAGACTCACC AGTTTTTTGT AAAATCTTTT ACTACTCCTA CCAAGTGTCA TCAGTGTACC 
TCCTTGATGG TGGGTTTAAT AAGACAGGGC TGTTCATGTG AAGTGTGTGG ATTCTCATGC 
CATATAACTT GTGTAAACAA AGCTCCAACC ACTTGTCCAG TTCCTCCTGA ACAGACAAAA 
GGTCCCCTGG GTATAGATCC TCAGAAAGGA ATAGGAACAG CATATGAAGG TCATGTCAGG 
ATTCCTAAGC CAGCTGGAGT GAAGAAAGGG TGGCAGAGAG CACTGGCTAT AGTGTGTGAC 
TTCAAACTCT TTCTGTACGA TATTGCTGAA GGAAAAGCAT CTCAGCCCAG TGTTGTCATT 
AGTCAAGTGA TTGACATGAG GGATGAAGAA TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT 
GTTATCCATG CAAGTCGGAA AGATATACCC TGTATATTTA GGGTCACAGC TTCCCAGCTC 
TCAGCATCTA ATAACAAATG TTCAATCCTG ATGCTAGCAG ACACTGAGAA TGAGAAGAAT 
AAGTGGGTGG GAGTGCTGAG TGAATTGCAC AAGATTTTGA AGAAAAACAA ATTCAGAGAC 
CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT GACAGCACTC TACCCCTCAT TAAAACAACC 
CAGGCAGCCG CAATCATAGA TCATGAAAGA ATTGCTTTGG GAAACGAAGA AGGGTTATTT 
GTTGTACATG TCACCAAAGA TGAAATTATT AGAGTTGGTG ACAATAAGAA GATTCATCAG 
ATTGAACTCA TTCCAAATGA TCAGCTTGTT GCTGTGATCT CAGGACGAAA TCGTCATGTA 
CGACTTTTTC CTATGTCAGC ATTGGATGGG CGAGAGACCG ATTTTTACAA GCTGTCAGAA 
ACTAAAGGGT GTCAAACCGT AACTTCTGGA AAGGTGCGCC ATGGAGCTCT CACATGCCTG 
TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT TATGAACTAT TTCAGAGCAA GACCCGTCAC 
AGAAAATTTA AAGAAATTCA AGTCCCATAT AATGTCCAGT GGATGGCAAT CTTCAGTGAA 
CAACTCTGTG TGGGATTCCA GTCAGGATTT CTAAGATACC CCTTGAATGG AGAAGGAAAT 
CCATACAGTA TGCTCCATTC AAATGACCAT ACACTATCAT TTATTGCACA TCAACCAATG 
GATGCTATCT GCGCAGTTGA GATCTCCAGT AAAGAATATC TGCTGTGTTT TAACAGCATT 
GGGATATACA CTGACTGCCA GGGCCGAAGA TCTAGACAAC AGGAATTGAT GTGGCCAGCA 
AATCCTTCCT CTTGTTGTTA CAATGCACCA TATCTCTCGG TGTACAGTGA AAATGCAGTT 
GATATCTTTG ATGTGAACTC CATGGAATGG ATTCAGACTC TTCCTCTCAA AAAGGTTCGA 
CCCTTAAACA ATGAAGGATC ATTAAATCTT TTAGGGTTGG AGACCATTAG ATTAATATAT 
TTCAAAAATA AGATGGCAGA AGGGGACGAA CTGGTAGTAC CTGAAACATC AGATAATAGT 
CGGAAACAAA TGGTTAGAAA CATTAACAAT AAGCGGCGTT ATTCCTTCAG AGTCCCAGAA 
GAGGAAAGGA TGCAGCAGAG GAGGGAAATG CTACGAGATC CAGAAATGAG AAATAAATTA 
ATTTCTAATC CAACTAATTT TAATCACATA GCACACATGG GTCCTGGAGA TGGAATACAG 
ATCCTGAAAG ATCTGCCCAT GCCAGGTTTC CCTTATCCAT CCCCTCATCA TCACTCCGGT 
CTGATCTCCT CTCCGATCAA CTTTGAACAC ATCTATCACA TGACTGTTAA TTCTGCTGAA 
AAATTTCTCT CTCCTGATTC CATAAACCCT GAATATTCCC CGTCTCTGCG CTCTGTCCCT 
GGTACACCAA GCTTTATGAC TCTGAGGAAC CCTCGGCCTC AGGAAAGTCG GACAGTATTC 
AGTGGCTCAG TCAGTATTCC ATCTATCACC AAATCCCGCC CTGAGCCAGG CCGCTCCATG 
AGTGCTAGCA GTGGCTTGTC AGCAAGGTCA TCCGCACAGA ATGGCAGCGC ATTAAAGAGG 
GAATTCTCTG GAGGAAGCTA CAGTGCCAAG CGGCAGCCCA TGCCCTCCCC GTCAGAGGGC 
TCTTTGTCCT CTGGAGGCAT GGACCAAGGA AGTGATGCCC CAGCGAGGGA CTTTGACGGA 
GAGGACTCTG ACTCTCCGAG GCATTCCACA GCTTCCAACA GTTCCAACCT AAGCAGCCCC 
CCAAGCCCAG CTTCACCCCG AAAAACCAAG AGCCTCTCCC TGGAGAGCAC TGACCGCGGG 
AGCTGGGACC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542611.1
CDS	1056..6350
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKLEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVENTYVWNPSVKFHIQSRSTSPSTSSEAEPVKTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_011544309.3
Organism	Homo sapiens(human)
Definition	Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544309.3

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAGACA TTACTGGATA TACTCATCTG CCTTTATGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TCGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAAATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TAAAACTAAA AAATGCAGAT 
AAAGTGTTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACCTTG 
CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGATAT GGCTAGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAC TCAGTTCATC AGCTACATTA TGTACACAGA 
GACATTAAAC CTGACAATAT ACTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTTCAGT CCTCAGTGGC TGTAGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG GAGATATGGA 
CCTGAATGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA CGGAGAAACA 
CCATTTTATG CAGAATCGCT GGTGGAGACA TACGGAAAAA TCATGAACCA CAAAGAGAGG 
TTTCAGTTTC CAGCCCAAGT GACTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGG 
CTCATTTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA GTGGAATTGA TTGGGATAAT ATTCGGAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTAG ATGATGATTG TTTAAAAAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCATTTTCTG GCCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTACTT TCTGATCGGA GCTGTTTAAG AGTTACGGCT 
GGTCCCACCT CACTGGATCT TGATGTTAAT GTTCAGAGGA CTCTAGACAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAAG AAAAACTTGA ACTCAGTAGA 
AAACTTCAAG AGTCAACACA GACTGTCCAA GCTCTGCAGT ATTCAACTGT TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AACTTAAAAG AAGAAATTGA AAAACTAAGA 
AAACAAGTAA CAGAATCAAG TCATTTGGAA CAGCAACTTG AAGAAGCTAA TGCTGTGAGG 
CAAGAACTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAAACGTTA 
CAACAAGAAA GAGAAGATCT AAATAAGCTG GAAGTTCATA CAGAAGCTCT AGCTGCTGAA 
GCATCTAAAG ACAGGAAGCT ACGTGAACAG AGTGAGCACT ATTCTAAGCA ACTGGAAAAT 
GAATTGGAGG GACTGAAGCA AAAACAAATT AGTTACTCAC CAGGAGTATG CAGCATAGAA 
CATCAGCAAG AGATAACCAA ACTAAAGACT GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAATTAT CTAAAAGAGA AGGAATACAT GCAAATGAAA TAAAAAATCT TAAGAAAGAA 
CTGCATGATT CAGAAGGTCA GCAACTTGCT CTCAACAAAG AAATTATGAT TTTAAAAGAC 
AAATTGGAAA AAACCAGAAG AGAAAGTCAA AGTGAAAGGG AGGAATTTGA AAGTGAGTTC 
AAACAACAAT ATGAACGAGA AAAAGTGTTG TTAACTGAAG AAAATAAAAA GCTGACGAGT 
GAACTTGATA AGCTTACTAC TTTGTATGAG AACTTAAGTA TACACAACCA GCAGTTAGAA 
GAAGAGGTTA AAGATCTAGC AGACAAGAAA GAATCAGTTG CACATTGGGA AGCCCAAATC 
ACAGAAATAA TTCAGTGGGT CAGCGATGAA AAGGATGCAC GAGGGTATCT TCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAGGCA TTAAGAAATT CCAGCTTGGG TACACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCGAAACTGG ATATGTCAGC TAGACTGGAG 
TTGCAGTCGG CTCTGGATGC AGAAATAAGA GCCAAACAGG CCATCCAAGA AGAGTTGAAT 
AAAGTTAAAG CATCTAATAT CATAACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAACTAC TCTCAGAAAT CGAACAGCTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AGATTCCTTT TCTTCTTCCT CATCTTCACT GATTGATTTT 
TTGGATGACC GCTCTCCATC CTGTACTCCA GCTAGCAAAG GCAGACGTAC TGATCCCGTT 
GAGAACACAT ATGTATGGAA CCCGAGCGTC AAGTTTCACA TCCAGTCACG GTCCACATCT 
CCTTCCACAT CTAGTGAAGC TGAGCCAGTT AAGACTGTAG ACTCCACTCC ACTTTCAGTT 
CACACACCAA CCTTAAGGAA AAAAGGATGT CCTGGTTCAA CTGGCTTTCC ACCTAAGCGC 
AAGACTCACC AGTTTTTTGT AAAATCTTTT ACTACTCCTA CCAAGTGTCA TCAGTGTACC 
TCCTTGATGG TGGGTTTAAT AAGACAGGGC TGTTCATGTG AAGTGTGTGG ATTCTCATGC 
CATATAACTT GTGTAAACAA AGCTCCAACC ACTTGTCCAG TTCCTCCTGA ACAGACAAAA 
GGTCCCCTGG GTATAGATCC TCAGAAAGGA ATAGGAACAG CATATGAAGG TCATGTCAGG 
ATTCCTAAGC CAGCTGGAGT GAAGAAAGGG TGGCAGAGAG CACTGGCTAT AGTGTGTGAC 
TTCAAACTCT TTCTGTACGA TATTGCTGAA GGAAAAGCAT CTCAGCCCAG TGTTGTCATT 
AGTCAAGTGA TTGACATGAG GGATGAAGAA TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT 
GTTATCCATG CAAGTCGGAA AGATATACCC TGTATATTTA GGGTCACAGC TTCCCAGCTC 
TCAGCATCTA ATAACAAATG TTCAATCCTG ATGCTAGCAG ACACTGAGAA TGAGAAGAAT 
AAGTGGGTGG GAGTGCTGAG TGAATTGCAC AAGATTTTGA AGAAAAACAA ATTCAGAGAC 
CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT GACAGCACTC TACCCCTCAT TAAAACAACC 
CAGGCAGCCG CAATCATAGA TCATGAAAGA ATTGCTTTGG GAAACGAAGA AGGGTTATTT 
GTTGTACATG TCACCAAAGA TGAAATTATT AGAGTTGGTG ACAATAAGAA GATTCATCAG 
ATTGAACTCA TTCCAAATGA TCAGCTTGTT GCTGTGATCT CAGGACGAAA TCGTCATGTA 
CGACTTTTTC CTATGTCAGC ATTGGATGGG CGAGAGACCG ATTTTTACAA GCTGTCAGAA 
ACTAAAGGGT GTCAAACCGT AACTTCTGGA AAGGTGCGCC ATGGAGCTCT CACATGCCTG 
TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT TATGAACTAT TTCAGAGCAA GACCCGTCAC 
AGAAAATTTA AAGAAATTCA AGTCCCATAT AATGTCCAGT GGATGGCAAT CTTCAGTGAA 
CAACTCTGTG TGGGATTCCA GTCAGGATTT CTAAGATACC CCTTGAATGG AGAAGGAAAT 
CCATACAGTA TGCTCCATTC AAATGACCAT ACACTATCAT TTATTGCACA TCAACCAATG 
GATGCTATCT GCGCAGTTGA GATCTCCAGT AAAGAATATC TGCTGTGTTT TAACAGCATT 
GGGATATACA CTGACTGCCA GGGCCGAAGA TCTAGACAAC AGGAATTGAT GTGGCCAGCA 
AATCCTTCCT CTTGTTGTTA CAATGCACCA TATCTCTCGG TGTACAGTGA AAATGCAGTT 
GATATCTTTG ATGTGAACTC CATGGAATGG ATTCAGACTC TTCCTCTCAA AAAGGTTCGA 
CCCTTAAACA ATGAAGGATC ATTAAATCTT TTAGGGTTGG AGACCATTAG ATTAATATAT 
TTCAAAAATA AGATGGCAGA AGGGGACGAA CTGGTAGTAC CTGAAACATC AGATAATAGT 
CGGAAACAAA TGGTTAGAAA CATTAACAAT AAGCGGCGTT ATTCCTTCAG AGTCCCAGAA 
GAGGAAAGGA TGCAGCAGAG GAGGGAAATG CTACGAGATC CAGAAATGAG AAATAAATTA 
ATTTCTAATC CAACTAATTT TAATCACATA GCACACATGG GTCCTGGAGA TGGAATACAG 
ATCCTGAAAG ATCTGCCCAT GCCAGGTTTC CCTTATCCAT CCCCTCATCA TCACTCCGGT 
CTGATCTCCT CTCCGATCAA CTTTGAACAC ATCTATCACA TGACTGTTAA TTCTGCTGAA 
AAATTTCTCT CTCCTGATTC CATAAACCCT GAATATTCCC CGTCTCTGCG CTCTGTCCCT 
GGTACACCAA GCTTTATGAC TCTGAGGAAC CCTCGGCCTC AGGAAAGTCG GACAGTATTC 
AGTGGCTCAG TCAGTATTCC ATCTATCACC AAATCCCGCC CTGAGCCAGG CCGCTCCATG 
AGTGCTAGCA GTGGCTTGTC AGCAAGGTCA TCCGCACAGA ATGGCAGCGC ATTAAAGAGG 
GAATTCTCTG GAGGAAGCTA CAGTGCCAAG CGGCAGCCCA TGCCCTCCCC GTCAGAGGGC 
TCTTTGTCCT CTGGAGGCAT GGACCAAGGA AGTGATGCCC CAGCGAGGGA CTTTGACGGA 
GAGGACTCTG ACTCTCCGAG GCATTCCACA GCTTCCAACA GTTCCAACCT AAGCAGCCCC 
CCAAGCCCAG CTTCACCCCG AAAAACCAAG AGCCTCTCCC TGGAGAGCAC TGACCGCGGG 
AGCTGGGACC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MRCK? is activated by caspase cleavage to assemble an apical actin ring for epithelial cell extrusion.
The Journal of cell biology217(1)231-249(2018 Jan)
Gagliardi PA,Somale D,Puliafito A,Chiaverina G,di Blasio L,Oneto M,Bianchini P,Bussolino F,Primo L

Comparative Proteomics Analysis Identifies Cdc42-Cdc42BPA Signaling as Prognostic Biomarker and Therapeutic Target for Colon Cancer Invasion.
Journal of proteome research17(1)265-275(2018 Jan)
Hu HF,Xu WW,Wang Y,Zheng CC,Zhang WX,Li B,He QY

Inhibition of cell migration and invasion by miR?29a?3p in a colorectal cancer cell line through suppression of CDC42BPA mRNA expression.
Oncology reports38(6)3554-3566(2017 Dec)
He PY,Yip WK,Chai BL,Chai BY,Jabar MF,Dusa N,Mohtarrudin N,Seow HF

ICAP-1 monoubiquitylation coordinates matrix density and rigidity sensing for cell migration through ROCK2-MRCK? balance.
Journal of cell science130(3)626-636(2017 02)
Bouin AP,Kyumurkov A,R?gent-Kloeckner M,Ribba AS,Faurobert E,Fournier HN,Bourrin-Reynard I,Manet-Dup? S,Oddou C,Balland M,Planus E,Albiges-Rizo C

Discovery and characterization of a novel CCND1/MRCK gene fusion in mantle cell lymphoma.
Journal of hematology & oncology930(2016 Mar)
Masamha CP,Albrecht TR,Wagner EJ

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CDC42BPA cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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