CDC42BPA gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_003435000.3	XP_003435048.1	serine/threonine-protein kinase MRCK alpha isoform X19
XM_005622622.1	XP_005622679.1	serine/threonine-protein kinase MRCK alpha isoform X9
XM_005622627.1	XP_005622684.1	serine/threonine-protein kinase MRCK alpha isoform X13
XM_005622624.1	XP_005622681.1	serine/threonine-protein kinase MRCK alpha isoform X13
XM_005622627.2	XP_005622684.1	serine/threonine-protein kinase MRCK alpha isoform X17
XM_858573.2	XP_863666.1	serine/threonine-protein kinase MRCK alpha isoform X14
XM_005622614.1	XP_005622671.1	serine/threonine-protein kinase MRCK alpha isoform X1
XM_005622615.1	XP_005622672.1	serine/threonine-protein kinase MRCK alpha isoform X3
XM_005622617.1	XP_005622674.1	serine/threonine-protein kinase MRCK alpha isoform X5
XM_005622618.1	XP_005622675.1	serine/threonine-protein kinase MRCK alpha isoform X6
XM_005622622.2	XP_005622679.1	serine/threonine-protein kinase MRCK alpha isoform X11
XM_858483.2	XP_863576.1	serine/threonine-protein kinase MRCK alpha isoform X16
XM_022421171.1	XP_022276879.1	serine/threonine-protein kinase MRCK alpha isoform X2
XM_005622616.1	XP_005622673.1	serine/threonine-protein kinase MRCK alpha isoform X4
XM_005622620.1	XP_005622677.1	serine/threonine-protein kinase MRCK alpha isoform X8
XM_005622621.1	XP_005622678.1	serine/threonine-protein kinase MRCK alpha isoform X9
XM_005622623.1	XP_005622680.1	serine/threonine-protein kinase MRCK alpha isoform X12
XM_022421174.1	XP_022276882.1	serine/threonine-protein kinase MRCK alpha isoform X14
XM_005622625.1	XP_005622682.1	serine/threonine-protein kinase MRCK alpha isoform X15
XM_858573.3	XP_863666.1	serine/threonine-protein kinase MRCK alpha isoform X18
XM_858483.3	XP_863576.1	serine/threonine-protein kinase MRCK alpha isoform X20
XM_003435000.2	XP_003435048.1	serine/threonine-protein kinase MRCK alpha isoform X15
XM_005622619.1	XP_005622676.1	serine/threonine-protein kinase MRCK alpha isoform X7
XM_022421172.1	XP_022276880.1	serine/threonine-protein kinase MRCK alpha isoform X10
XM_022421175.1	XP_022276883.1	serine/threonine-protein kinase MRCK alpha isoform X16

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe09390	XM_022421175.1 Latest version!	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe289620	XM_022421171.1 Latest version!	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe289621	XM_022421172.1 Latest version!	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe289622	XM_022421174.1 Latest version!	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe09378	XM_005622614.1 Latest version!	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe09379	XM_005622615.1 Latest version!	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe09380	XM_005622616.1 Latest version!	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe09381	XM_005622617.1 Latest version!	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe09382	XM_005622618.1 Latest version!	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe09383	XM_005622619.1 Latest version!	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe09384	XM_005622620.1 Latest version!	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe09385	XM_005622621.1 Latest version!	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe09386	XM_005622622.2 Latest version!	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe09387	XM_005622623.1 Latest version!	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe09388	XM_005622624.1 Latest version!	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe09389	XM_005622625.1 Latest version!	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe09391	XM_005622627.2 Latest version!	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe09392	XM_858573.3 Latest version!	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe09393	XM_003435000.3 Latest version!	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe09394	XM_858483.3 Latest version!	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OCe09391	XM_005622627.1	Canis lupus familiaris CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe09386	XM_005622622.1	Canis lupus familiaris CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe09392	XM_858573.2	Canis lupus familiaris CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe09394	XM_858483.2	Canis lupus familiaris CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe09393	XM_003435000.2	Canis lupus familiaris CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

CDC42BPA ( XM_022421175.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022276883.1 Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OCe09390
Clone ID Related Accession (Same CDS sequence)	XM_022421175.1
Accession Version	XM_022421175.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase MRCK alpha isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATAT TGAATAAATG GGAAATGCTG AAAAGGGCTG AGACAGCATG TTTTCGTGAA 
GAAAGGGATG TATTAGTGAA TGGAGATAAT AAATGGATTA CAACTCTGCA TTATGCTTTC 
CAGGATGACA ATAACTTATA CTTGGTCATG GATTATTATG TTGGTGGGGA TTTGCTTACT 
CTGCTCAGCA AATTTGAAGA TCGATTGCCT GAAGATATGG CTCGATTTTA CTTGGCCGAG 
ATGGTGATTG CAATCGATTC TGTTCATCAG CTGCACTATG TGCATAGGGA CATCAAACCA 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGATTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCAGTGGCTG TTGGAACTCC AGATTATATC 
TCTCCTGAAA TCCTTCAAGC CATGGAAGAT GGGAAAGGAA GATACGGGCC TGAATGTGAC 
TGGTGGTCTC TGGGGGTCTG TATGTACGAA ATGCTTTATG GAGAAACACC ATTTTATGCA 
GAATCTCTGG TGGAGACCTA TGGAAAAATC ATGAATCATA AAGAGAGGTT TCAGTTTCCA 
GCTCAAGTGA CGGATGTGTC TGAAAATGCT AAAGATCTGA TTCGTAGGCT CATTTGCAGC 
CGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAACACCC ATTTTTCAAT 
GGAATTGATT GGGATAATAT TCGAAACTGT GAAGCACCGT ATATTCCAGA AGTTAGTAGC 
CCAACAGATA CATCGAATTT TGATGTGGAT GATGATTGTT TAAAGAATTC TGAAACGATG 
CCCCCACCCA CACATACTGC ATTTTCTGGC CACCACCTGC CATTTGTTGG TTTCACATAT 
ACCAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTACGGG TCACGGCTGG TCCTGCGTCC 
CTGGACCTTG ACGTCAGTGT CCAGCGGACT CTAGACAACA ACCTGGCCAC TGAGGCATAT 
GAGAGGAGAA TTAAGCGCCT CGAACAGGAG AAGCTGGAGC TGAGTCGGAA GCTTCAAGAG 
TCAACACAGA CTGTCCAAGC TCTACAGTAT TCAACTGCTG ATGGCCCACT AACAGCAAGC 
AAAGATTTAG AAATAAAAAC CTTGAAAGAA GAAATTGAAA ACCTAAGGAA ACAAATAAGA 
GAATCAAGTC ATTTGGAACA ACAACTTGAA GAAGCCAACT CTGTGAGGAG AGAGTTAGAT 
GATGCTTTTC GACAAATCAA GGCTTATGAA AAACAAATCA GAACACTTCA ACAAGAAAGG 
GAAGAATTAA ACAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA GTCCAAAGAG 
CTGAAAGACG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGGCTAACAG AATTGCACAC CCAAAAACAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGTT GAAAGCTTAA GGCAAGAACT GCGCAGAACA 
GAGAGAGCCA AAAAAGAGCT GGAAGTTCAT ACAGAAGCTG TGGCTGCTGA AGCATCTAAA 
GATAGGAAAC TGCGTGAACA GAGTGAGCAT TATTCTAAGC AACTGGAAAA TGAATTGGAG 
GGTCTAAAGC AGAAACAAAT TAGTTACTCC CCAGGACTGT GCAGCATAGA ACACCAGCAG 
GAGATATCCA AACTAAAGAC GGATTTGGAA AAGAAAAGTA TCTTTTATGA AGAGGAATTG 
TCTAAAAGAG AAGGAATACA TGCAAATGAA ATTAAAAATC TGAAGAAAGA ACTTCATGAT 
TCAGAAGGCC AGCAACTTGC TCTCAACAAG GAAATTATGA TTTTGAAAGA CAAATTAGAG 
AAAAACAGAA GAGAGAGTCA AAGTGAAAGG GAAGAGTTTG AAAATGAATT CAAACAACAA 
TATGAACGAG AAAAAGTATT ATTAACAGAA GAAAATAAAA AACTGACAAG TGAACTTGAT 
AAGCTCACCA CTTTGTATGA AAATGTAAGT ATGCGCAACC AGCAGTTAGA AGAAGAGGTG 
AAAGACCTAG CAGACAAGAA AGAATCAGTT GCACATTGGG AAGCCCAAAT CACAGAAATA 
ATTCAGTGGG TCAGTGATGA AAAGGATGCA CGAGGGTATC TCCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AATTGGAAGC TTTGAGGAAT TCCAGCTTGG GTGCACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCAAAATTG GATATGTCAG CTAGACTGGA GTTGCAGTCA 
GCTCTGGATG CAGAAATAAG AGCTAAACAG GCCATCCAAG AAGAACTGAA TAAAGTTAAA 
GCATCTAACA TCATCACAGA ATGTAAACTA AAAGATTCAG AGAAGAAGAA CTTGGAGCTC 
CTCTCAGAAA TCGAACAACT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GATCAATTTG AAGATTCCTT TTCTTCTTCC TCATCTTCAC TGATTGATTT TTTGGATGAC 
CGCTCTCCGT CCTGTACTCC AGCTAGCAAA GGCAGACGTA CCGACCCCGT TGAGAGCACA 
TATGTACGGA ATCCGAACGT CAAGGTTAAC GTCCAGTCAA GGTCCACATC TCCTTCTACA 
TCTAGTGAAG CTGAGCCACT TAAGATTGTA GACAGTACTC CTCTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG TCCTGGTTCA ACTGGCTTTC CAGCTAAGCG CAAGACTCAT 
CAGTTTTTTG TCAAATCTTT CACAACACCT ACCAGATGTC ACCAGTGCAC CTCTCTGATG 
GTTGGTTTGA TAAGACAAGG CTGTTCCTGT GAGGTGTGCG GATTCTCATG TCATATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTCCCTCCTG AGCAGACAAA AGGTCCTCTG 
GGGATAGATC CACAGAAAGG AATAGGAACA GCCTATGAAG GTCATGTCAG GATTCCGAAA 
CCAGCTGGAG TAAAGAAAGG ATGGCAGAGA GCCCTGGCTG TGGTTTGTGA CTTTAAACTC 
TTCCTGTATG ATGTCGCTGA AGGAAAAGCA TCTCAGCCCA GCGTTGTGGT CAGTCAGGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTGT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTC AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
GACAGCAGGT GTTCGGTCCT AATGCTCGCA GAGAGTGAGA GCGAGAGGAA CAAGTGGGTG 
GGCGTGCTCA ACGAGCTGCA TAAGATTTTG AAGAAAAGCA AATTCAGAGA CCGCTCCGTC 
TATGTTCCCA AGGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCACGAAAG GATAGCTCTG GGCAATGAAG AAGGATTATT TGTTGTGCAC 
ATCACCAAAG ATGAAATTAT TAGAGTTGGT GATAATAAGA AGATACATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATATCAG CTTTGGACGG ACGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGG 
TGTCAGACCA TAACTTCGGG CAAAGTGTGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGGAAATTT 
AAGGAAATTC AAGTCCCATA TAACGTGCAA TGGATGGCGA TCTTCAGTGA ACAGCTCTGC 
GTGGGATTCC AGTCGGGATT CCTAAGATAC CCGTTGAACG GAGAGGGAAG CCCATACAGT 
ATGCTCCATT CAAATGACCA CACACTGTCA TTTATTGCCC ACCAGCCAAT GGATGCCATC 
TGCGCAGTTG AGATCTCCAG TAAAGAGTAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGGAG ATCGAGACAA CAGGAGTTGA TGTGGCCGTC AAATCCCTCC 
TCTTGTTGTT ACAACGCACC ATATCTCTCA GTGTATAGTG AGAATGCAGT TGATATCTTC 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG ACCCTTAAAC 
AGTGAAGGAT CATTAAACCT TTTAGGGCTG GAGACAATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTGGTGGTT CCTGAAACAT CGGACAATAG TCGGAAGCAG 
ATGGTTAGAA ACATCAACAA CAAGCGACGC TTCTCCTTCA GGGTCCCAGA AGAGGAGAGG 
ATGCAGCAGA GGAGAGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGCCCAGGGG ATGGAATACA GATCCTAAAA 
GACCTGCCTA TGCCAGGCTT CCCTTATCCA TCCCCAAATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAGCA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGCATATTCC CCGTCTCTGC GCTCTGTCCC CGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGCC GGACCGTGTT CAGCGGCTCA 
GTCAGTATTC CATCCATCAC TAAATCCCGC CCTGAGCCCG GCCGCTCCAT GAGCGCCAGC 
AGCGGCCTTT CGGCAAGGTC ATCTGCACAG AATGGCAGTG CATTAAAGAG AGAATTCTCC 
GGAGGAAGCT ATAATGCTAA GTGGCAGCCC ATGCCCTCCC CATCAGAGGG CTCCTTGTCT 
TCCGGAGGCA TGGACCAGGG AAGTGACGCC CCAGCCAGGG ACTATGACGG AGAGGACTCG 
GATTCTCCGA GGCACTCCAC AGCTTCCAAC AGCTCCAACC TCAGCAGCCC GCCCAGCCCA 
GTGTCACCTC GGAAGACCAA GAGCCTGTCC CTGGAGAGCA CCGACCGTGG GACCTGGGAC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276883.1
CDS	293..5518
Translation	MKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPASLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKTLKEEIENLRKQIRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIRTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGLCSIEHQQEISKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLYENVSMRNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGARATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVESTYVRNPNVKVNVQSRSTSPSTSSEAEPLKIVDSTPLPAHTPTLRKKGCPGSTGFPAKRKTHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDVAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASDSRCSVLMLAESESERNKWVGVLNELHKILKKSKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHITKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPISALDGRETDFYKLAETKGCQTITSGKVCHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPSNPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRFSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPNHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPAYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNAKWQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGTWDP

Target ORF information:

RefSeq Version	XM_022421175.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421175.1

ORF Insert Sequence:

ATGAAAATAT TGAATAAATG GGAAATGCTG AAAAGGGCTG AGACAGCATG TTTTCGTGAA 
GAAAGGGATG TATTAGTGAA TGGAGATAAT AAATGGATTA CAACTCTGCA TTATGCTTTC 
CAGGATGACA ATAACTTATA CTTGGTCATG GATTATTATG TTGGTGGGGA TTTGCTTACT 
CTGCTCAGCA AATTTGAAGA TCGATTGCCT GAAGATATGG CTCGATTTTA CTTGGCCGAG 
ATGGTGATTG CAATCGATTC TGTTCATCAG CTGCACTATG TGCATAGGGA CATCAAACCA 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGATTAG CAGATTTTGG TTCTTGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAGTCC TCAGTGGCTG TTGGAACTCC AGATTATATC 
TCTCCTGAAA TCCTTCAAGC CATGGAAGAT GGGAAAGGAA GATACGGGCC TGAATGTGAC 
TGGTGGTCTC TGGGGGTCTG TATGTACGAA ATGCTTTATG GAGAAACACC ATTTTATGCA 
GAATCTCTGG TGGAGACCTA TGGAAAAATC ATGAATCATA AAGAGAGGTT TCAGTTTCCA 
GCTCAAGTGA CGGATGTGTC TGAAAATGCT AAAGATCTGA TTCGTAGGCT CATTTGCAGC 
CGAGAACATC GACTTGGCCA AAATGGAATA GAAGACTTTA AGAAACACCC ATTTTTCAAT 
GGAATTGATT GGGATAATAT TCGAAACTGT GAAGCACCGT ATATTCCAGA AGTTAGTAGC 
CCAACAGATA CATCGAATTT TGATGTGGAT GATGATTGTT TAAAGAATTC TGAAACGATG 
CCCCCACCCA CACATACTGC ATTTTCTGGC CACCACCTGC CATTTGTTGG TTTCACATAT 
ACCAGTAGCT GTGTTCTTTC TGATCGGAGC TGTTTACGGG TCACGGCTGG TCCTGCGTCC 
CTGGACCTTG ACGTCAGTGT CCAGCGGACT CTAGACAACA ACCTGGCCAC TGAGGCATAT 
GAGAGGAGAA TTAAGCGCCT CGAACAGGAG AAGCTGGAGC TGAGTCGGAA GCTTCAAGAG 
TCAACACAGA CTGTCCAAGC TCTACAGTAT TCAACTGCTG ATGGCCCACT AACAGCAAGC 
AAAGATTTAG AAATAAAAAC CTTGAAAGAA GAAATTGAAA ACCTAAGGAA ACAAATAAGA 
GAATCAAGTC ATTTGGAACA ACAACTTGAA GAAGCCAACT CTGTGAGGAG AGAGTTAGAT 
GATGCTTTTC GACAAATCAA GGCTTATGAA AAACAAATCA GAACACTTCA ACAAGAAAGG 
GAAGAATTAA ACAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA GTCCAAAGAG 
CTGAAAGACG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGGCTAACAG AATTGCACAC CCAAAAACAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGTT GAAAGCTTAA GGCAAGAACT GCGCAGAACA 
GAGAGAGCCA AAAAAGAGCT GGAAGTTCAT ACAGAAGCTG TGGCTGCTGA AGCATCTAAA 
GATAGGAAAC TGCGTGAACA GAGTGAGCAT TATTCTAAGC AACTGGAAAA TGAATTGGAG 
GGTCTAAAGC AGAAACAAAT TAGTTACTCC CCAGGACTGT GCAGCATAGA ACACCAGCAG 
GAGATATCCA AACTAAAGAC GGATTTGGAA AAGAAAAGTA TCTTTTATGA AGAGGAATTG 
TCTAAAAGAG AAGGAATACA TGCAAATGAA ATTAAAAATC TGAAGAAAGA ACTTCATGAT 
TCAGAAGGCC AGCAACTTGC TCTCAACAAG GAAATTATGA TTTTGAAAGA CAAATTAGAG 
AAAAACAGAA GAGAGAGTCA AAGTGAAAGG GAAGAGTTTG AAAATGAATT CAAACAACAA 
TATGAACGAG AAAAAGTATT ATTAACAGAA GAAAATAAAA AACTGACAAG TGAACTTGAT 
AAGCTCACCA CTTTGTATGA AAATGTAAGT ATGCGCAACC AGCAGTTAGA AGAAGAGGTG 
AAAGACCTAG CAGACAAGAA AGAATCAGTT GCACATTGGG AAGCCCAAAT CACAGAAATA 
ATTCAGTGGG TCAGTGATGA AAAGGATGCA CGAGGGTATC TCCAGGCCTT AGCTTCTAAA 
ATGACTGAAG AATTGGAAGC TTTGAGGAAT TCCAGCTTGG GTGCACGAGC AACAGATATG 
CCCTGGAAAA TGCGTCGTTT TGCAAAATTG GATATGTCAG CTAGACTGGA GTTGCAGTCA 
GCTCTGGATG CAGAAATAAG AGCTAAACAG GCCATCCAAG AAGAACTGAA TAAAGTTAAA 
GCATCTAACA TCATCACAGA ATGTAAACTA AAAGATTCAG AGAAGAAGAA CTTGGAGCTC 
CTCTCAGAAA TCGAACAACT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GATCAATTTG AAGATTCCTT TTCTTCTTCC TCATCTTCAC TGATTGATTT TTTGGATGAC 
CGCTCTCCGT CCTGTACTCC AGCTAGCAAA GGCAGACGTA CCGACCCCGT TGAGAGCACA 
TATGTACGGA ATCCGAACGT CAAGGTTAAC GTCCAGTCAA GGTCCACATC TCCTTCTACA 
TCTAGTGAAG CTGAGCCACT TAAGATTGTA GACAGTACTC CTCTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG TCCTGGTTCA ACTGGCTTTC CAGCTAAGCG CAAGACTCAT 
CAGTTTTTTG TCAAATCTTT CACAACACCT ACCAGATGTC ACCAGTGCAC CTCTCTGATG 
GTTGGTTTGA TAAGACAAGG CTGTTCCTGT GAGGTGTGCG GATTCTCATG TCATATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTCCCTCCTG AGCAGACAAA AGGTCCTCTG 
GGGATAGATC CACAGAAAGG AATAGGAACA GCCTATGAAG GTCATGTCAG GATTCCGAAA 
CCAGCTGGAG TAAAGAAAGG ATGGCAGAGA GCCCTGGCTG TGGTTTGTGA CTTTAAACTC 
TTCCTGTATG ATGTCGCTGA AGGAAAAGCA TCTCAGCCCA GCGTTGTGGT CAGTCAGGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTGT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTC AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
GACAGCAGGT GTTCGGTCCT AATGCTCGCA GAGAGTGAGA GCGAGAGGAA CAAGTGGGTG 
GGCGTGCTCA ACGAGCTGCA TAAGATTTTG AAGAAAAGCA AATTCAGAGA CCGCTCCGTC 
TATGTTCCCA AGGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCACGAAAG GATAGCTCTG GGCAATGAAG AAGGATTATT TGTTGTGCAC 
ATCACCAAAG ATGAAATTAT TAGAGTTGGT GATAATAAGA AGATACATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATATCAG CTTTGGACGG ACGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGG 
TGTCAGACCA TAACTTCGGG CAAAGTGTGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGGAAATTT 
AAGGAAATTC AAGTCCCATA TAACGTGCAA TGGATGGCGA TCTTCAGTGA ACAGCTCTGC 
GTGGGATTCC AGTCGGGATT CCTAAGATAC CCGTTGAACG GAGAGGGAAG CCCATACAGT 
ATGCTCCATT CAAATGACCA CACACTGTCA TTTATTGCCC ACCAGCCAAT GGATGCCATC 
TGCGCAGTTG AGATCTCCAG TAAAGAGTAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGGAG ATCGAGACAA CAGGAGTTGA TGTGGCCGTC AAATCCCTCC 
TCTTGTTGTT ACAACGCACC ATATCTCTCA GTGTATAGTG AGAATGCAGT TGATATCTTC 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG ACCCTTAAAC 
AGTGAAGGAT CATTAAACCT TTTAGGGCTG GAGACAATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTGGTGGTT CCTGAAACAT CGGACAATAG TCGGAAGCAG 
ATGGTTAGAA ACATCAACAA CAAGCGACGC TTCTCCTTCA GGGTCCCAGA AGAGGAGAGG 
ATGCAGCAGA GGAGAGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGCCCAGGGG ATGGAATACA GATCCTAAAA 
GACCTGCCTA TGCCAGGCTT CCCTTATCCA TCCCCAAATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAGCA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGCATATTCC CCGTCTCTGC GCTCTGTCCC CGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGCC GGACCGTGTT CAGCGGCTCA 
GTCAGTATTC CATCCATCAC TAAATCCCGC CCTGAGCCCG GCCGCTCCAT GAGCGCCAGC 
AGCGGCCTTT CGGCAAGGTC ATCTGCACAG AATGGCAGTG CATTAAAGAG AGAATTCTCC 
GGAGGAAGCT ATAATGCTAA GTGGCAGCCC ATGCCCTCCC CATCAGAGGG CTCCTTGTCT 
TCCGGAGGCA TGGACCAGGG AAGTGACGCC CCAGCCAGGG ACTATGACGG AGAGGACTCG 
GATTCTCCGA GGCACTCCAC AGCTTCCAAC AGCTCCAACC TCAGCAGCCC GCCCAGCCCA 
GTGTCACCTC GGAAGACCAA GAGCCTGTCC CTGGAGAGCA CCGACCGTGG GACCTGGGAC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_022421171.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022276879.1 Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X2, mRNA.

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CloneID	OCe289620
Clone ID Related Accession (Same CDS sequence)	XM_022421171.1
Accession Version	XM_022421171.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase MRCK alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GAAACAAATT AGTTACTCCC CAGGACTGTG CAGCATAGAA 
CACCAGCAGG AGATATCCAA ACTAAAGACG GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAGGAATTGT CTAAAAGAGA AGGAATACAT GCAAATGAAA TTAAAAATCT GAAGAAAGAA 
CTTCATGATT CAGAAGGCCA GCAACTTGCT CTCAACAAGG AAATTATGAT TTTGAAAGAC 
AAATTAGAGA AAAACAGAAG AGAGAGTCAA AGTGAAAGGG AAGAGTTTGA AAATGAATTC 
AAACAACAAT ATGAACGAGA AAAAGTATTA TTAACAGAAG AAAATAAAAA ACTGACAAGT 
GAACTTGATA AGCTCACCAC TTTGTATGAA AATGTAAGTA TGCGCAACCA GCAGTTAGAA 
GAAGAGGTGA AAGACCTAGC AGACAAGAAA GAATCAGTTG CACATTGGGA AGCCCAAATC 
ACAGAAATAA TTCAGTGGGT CAGTGATGAA AAGGATGCAC GAGGGTATCT CCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAAGCT TTGAGGAATT CCAGCTTGGG TGCACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCAAAATTGG ATATGTCAGC TAGACTGGAG 
TTGCAGTCAG CTCTGGATGC AGAAATAAGA GCTAAACAGG CCATCCAAGA AGAACTGAAT 
AAAGTTAAAG CATCTAACAT CATCACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAGCTCC TCTCAGAAAT CGAACAACTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AGATTCCTTT TCTTCTTCCT CATCTTCACT GATTGATTTT 
TTGGATGACC GCTCTCCGTC CTGTACTCCA GCTAGCAAAG GCAGACGTAC CGACCCCGTT 
GAGAGCACAT ATGTACGGAA TCCGAACGTC AAGGTTAACG TCCAGTCAAG GTCCACATCT 
CCTTCTACAT CTAGTGAAGC TGAGCCACTT AAGATTGTAG ACAGTACTCC TCTTCCAGCT 
CACACACCAA CCTTAAGGAA AAAGGGATGT CCTGGTTCAA CTGGCTTTCC AGCTAAGCGC 
AAGACTCATC AGTTTTTTGT CAAATCTTTC ACAACACCTA CCAGATGTCA CCAGTGCACC 
TCTCTGATGG TTGGTTTGAT AAGACAAGGC TGTTCCTGTG AGGTGTGCGG ATTCTCATGT 
CATATAACTT GTGTAAACAA AGCTCCAACT GCTTGTCCAG TCCCTCCTGA GCAGACAAAA 
GGTCCTCTGG GGATAGATCC ACAGAAAGGA ATAGGAACAG CCTATGAAGG TCATGTCAGG 
ATTCCGAAAC CAGCTGGAGT AAAGAAAGGA TGGCAGAGAG CCCTGGCTGT GGTTTGTGAC 
TTTAAACTCT TCCTGTATGA TGTCGCTGAA GGAAAAGCAT CTCAGCCCAG CGTTGTGGTC 
AGTCAGGTGA TTGACATGAG GGATGAAGAA TTTTCTGTGA GTTCAGTGTT GGCTTCTGAT 
GTTATTCATG CAAGTCGAAA AGATATACCC TGTATATTCA GAGTCACAGC TTCCCAGCTC 
TCAGCATCTG ACAGCAGGTG TTCGGTCCTA ATGCTCGCAG AGAGTGAGAG CGAGAGGAAC 
AAGTGGGTGG GCGTGCTCAA CGAGCTGCAT AAGATTTTGA AGAAAAGCAA ATTCAGAGAC 
CGCTCCGTCT ATGTTCCCAA GGAGGCTTAT GACAGCACTC TGCCCCTCAT TAAAACAACC 
CAGGCAGCTG CAATCATAGA TCACGAAAGG ATAGCTCTGG GCAATGAAGA AGGATTATTT 
GTTGTGCACA TCACCAAAGA TGAAATTATT AGAGTTGGTG ATAATAAGAA GATACATCAG 
ATTGAACTCA TTCCAAATGA TCAGCTTGTT GCTGTGATCT CAGGACGAAA TCGTCATGTA 
CGACTTTTTC CTATATCAGC TTTGGACGGA CGAGAGACTG ATTTTTATAA GCTGGCAGAA 
ACTAAAGGGT GTCAGACCAT AACTTCGGGC AAAGTGTGCC ATGGAGCTCT CACATGCCTG 
TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT TATGAACTAT TTCAGAGCAA GACCCGTCAC 
AGGAAATTTA AGGAAATTCA AGTCCCATAT AACGTGCAAT GGATGGCGAT CTTCAGTGAA 
CAGCTCTGCG TGGGATTCCA GTCGGGATTC CTAAGATACC CGTTGAACGG AGAGGGAAGC 
CCATACAGTA TGCTCCATTC AAATGACCAC ACACTGTCAT TTATTGCCCA CCAGCCAATG 
GATGCCATCT GCGCAGTTGA GATCTCCAGT AAAGAGTATC TGCTGTGTTT TAACAGCATT 
GGGATATACA CTGACTGCCA GGGCCGGAGA TCGAGACAAC AGGAGTTGAT GTGGCCGTCA 
AATCCCTCCT CTTGTTGTTA CAACGCACCA TATCTCTCAG TGTATAGTGA GAATGCAGTT 
GATATCTTCG ATGTGAACTC CATGGAATGG ATTCAGACTC TCCCACTCAA AAAGGTTCGA 
CCCTTAAACA GTGAAGGATC ATTAAACCTT TTAGGGCTGG AGACAATTAG ATTAATATAT 
TTCAAAAATA AGATGGCAGA AGGGGATGAA CTGGTGGTTC CTGAAACATC GGACAATAGT 
CGGAAGCAGA TGGTTAGAAA CATCAACAAC AAGCGACGCT TCTCCTTCAG GGTCCCAGAA 
GAGGAGAGGA TGCAGCAGAG GAGAGAGATG CTACGAGATC CAGAAATGAG AAATAAATTA 
ATTTCTAACC CAACTAATTT TAACCACATA GCACACATGG GCCCAGGGGA TGGAATACAG 
ATCCTAAAAG ACCTGCCTAT GCCAGGCTTC CCTTATCCAT CCCCAAATCA TCACTCCGGT 
CTGATCTCCT CTCCGATCAA CTTTGAGCAC ATCTATCACA TGACTGTTAA TTCTGCTGAA 
AAATTTCTCT CTCCTGATTC CATAAACCCT GCATATTCCC CGTCTCTGCG CTCTGTCCCC 
GGTACACCAA GCTTTATGAC TCTGAGGAAC CCTCGGCCTC AGGAAAGCCG GACCGTGTTC 
AGCGGCTCAG TCAGTATTCC ATCCATCACT AAATCCCGCC CTGAGCCCGG CCGCTCCATG 
AGCGCCAGCA GCGGCCTTTC GGCAAGGTCA TCTGCACAGA ATGGCAGTGC ATTAAAGAGA 
GAATTCTCCG GAGGAAGCTA TAATGCTAAG TGGCAGCCCA TGCCCTCCCC ATCAGAGGGC 
TCCTTGTCTT CCGGAGGCAT GGACCAGGGA AGTGACGCCC CAGCCAGGGA CTATGACGGA 
GAGGACTCGG ATTCTCCGAG GCACTCCACA GCTTCCAACA GCTCCAACCT CAGCAGCCCG 
CCCAGCCCAG TGTCACCTCG GAAGACCAAG AGCCTGTCCC TGGAGAGCAC CGACCGTGGG 
ACCTGGGACC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276879.1
CDS	384..5918
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPASLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKTLKEEIENLRKQIRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIRTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEAVAAEASKDRKLREQSEHYSKQLENELEGLKKQISYSPGLCSIEHQQEISKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLYENVSMRNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGARATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVESTYVRNPNVKVNVQSRSTSPSTSSEAEPLKIVDSTPLPAHTPTLRKKGCPGSTGFPAKRKTHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDVAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASDSRCSVLMLAESESERNKWVGVLNELHKILKKSKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHITKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPISALDGRETDFYKLAETKGCQTITSGKVCHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPSNPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRFSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPNHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPAYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNAKWQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGTWDP

Target ORF information:

RefSeq Version	XM_022421171.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421171.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GAAACAAATT AGTTACTCCC CAGGACTGTG CAGCATAGAA 
CACCAGCAGG AGATATCCAA ACTAAAGACG GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAGGAATTGT CTAAAAGAGA AGGAATACAT GCAAATGAAA TTAAAAATCT GAAGAAAGAA 
CTTCATGATT CAGAAGGCCA GCAACTTGCT CTCAACAAGG AAATTATGAT TTTGAAAGAC 
AAATTAGAGA AAAACAGAAG AGAGAGTCAA AGTGAAAGGG AAGAGTTTGA AAATGAATTC 
AAACAACAAT ATGAACGAGA AAAAGTATTA TTAACAGAAG AAAATAAAAA ACTGACAAGT 
GAACTTGATA AGCTCACCAC TTTGTATGAA AATGTAAGTA TGCGCAACCA GCAGTTAGAA 
GAAGAGGTGA AAGACCTAGC AGACAAGAAA GAATCAGTTG CACATTGGGA AGCCCAAATC 
ACAGAAATAA TTCAGTGGGT CAGTGATGAA AAGGATGCAC GAGGGTATCT CCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAAGCT TTGAGGAATT CCAGCTTGGG TGCACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCAAAATTGG ATATGTCAGC TAGACTGGAG 
TTGCAGTCAG CTCTGGATGC AGAAATAAGA GCTAAACAGG CCATCCAAGA AGAACTGAAT 
AAAGTTAAAG CATCTAACAT CATCACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAGCTCC TCTCAGAAAT CGAACAACTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AGATTCCTTT TCTTCTTCCT CATCTTCACT GATTGATTTT 
TTGGATGACC GCTCTCCGTC CTGTACTCCA GCTAGCAAAG GCAGACGTAC CGACCCCGTT 
GAGAGCACAT ATGTACGGAA TCCGAACGTC AAGGTTAACG TCCAGTCAAG GTCCACATCT 
CCTTCTACAT CTAGTGAAGC TGAGCCACTT AAGATTGTAG ACAGTACTCC TCTTCCAGCT 
CACACACCAA CCTTAAGGAA AAAGGGATGT CCTGGTTCAA CTGGCTTTCC AGCTAAGCGC 
AAGACTCATC AGTTTTTTGT CAAATCTTTC ACAACACCTA CCAGATGTCA CCAGTGCACC 
TCTCTGATGG TTGGTTTGAT AAGACAAGGC TGTTCCTGTG AGGTGTGCGG ATTCTCATGT 
CATATAACTT GTGTAAACAA AGCTCCAACT GCTTGTCCAG TCCCTCCTGA GCAGACAAAA 
GGTCCTCTGG GGATAGATCC ACAGAAAGGA ATAGGAACAG CCTATGAAGG TCATGTCAGG 
ATTCCGAAAC CAGCTGGAGT AAAGAAAGGA TGGCAGAGAG CCCTGGCTGT GGTTTGTGAC 
TTTAAACTCT TCCTGTATGA TGTCGCTGAA GGAAAAGCAT CTCAGCCCAG CGTTGTGGTC 
AGTCAGGTGA TTGACATGAG GGATGAAGAA TTTTCTGTGA GTTCAGTGTT GGCTTCTGAT 
GTTATTCATG CAAGTCGAAA AGATATACCC TGTATATTCA GAGTCACAGC TTCCCAGCTC 
TCAGCATCTG ACAGCAGGTG TTCGGTCCTA ATGCTCGCAG AGAGTGAGAG CGAGAGGAAC 
AAGTGGGTGG GCGTGCTCAA CGAGCTGCAT AAGATTTTGA AGAAAAGCAA ATTCAGAGAC 
CGCTCCGTCT ATGTTCCCAA GGAGGCTTAT GACAGCACTC TGCCCCTCAT TAAAACAACC 
CAGGCAGCTG CAATCATAGA TCACGAAAGG ATAGCTCTGG GCAATGAAGA AGGATTATTT 
GTTGTGCACA TCACCAAAGA TGAAATTATT AGAGTTGGTG ATAATAAGAA GATACATCAG 
ATTGAACTCA TTCCAAATGA TCAGCTTGTT GCTGTGATCT CAGGACGAAA TCGTCATGTA 
CGACTTTTTC CTATATCAGC TTTGGACGGA CGAGAGACTG ATTTTTATAA GCTGGCAGAA 
ACTAAAGGGT GTCAGACCAT AACTTCGGGC AAAGTGTGCC ATGGAGCTCT CACATGCCTG 
TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT TATGAACTAT TTCAGAGCAA GACCCGTCAC 
AGGAAATTTA AGGAAATTCA AGTCCCATAT AACGTGCAAT GGATGGCGAT CTTCAGTGAA 
CAGCTCTGCG TGGGATTCCA GTCGGGATTC CTAAGATACC CGTTGAACGG AGAGGGAAGC 
CCATACAGTA TGCTCCATTC AAATGACCAC ACACTGTCAT TTATTGCCCA CCAGCCAATG 
GATGCCATCT GCGCAGTTGA GATCTCCAGT AAAGAGTATC TGCTGTGTTT TAACAGCATT 
GGGATATACA CTGACTGCCA GGGCCGGAGA TCGAGACAAC AGGAGTTGAT GTGGCCGTCA 
AATCCCTCCT CTTGTTGTTA CAACGCACCA TATCTCTCAG TGTATAGTGA GAATGCAGTT 
GATATCTTCG ATGTGAACTC CATGGAATGG ATTCAGACTC TCCCACTCAA AAAGGTTCGA 
CCCTTAAACA GTGAAGGATC ATTAAACCTT TTAGGGCTGG AGACAATTAG ATTAATATAT 
TTCAAAAATA AGATGGCAGA AGGGGATGAA CTGGTGGTTC CTGAAACATC GGACAATAGT 
CGGAAGCAGA TGGTTAGAAA CATCAACAAC AAGCGACGCT TCTCCTTCAG GGTCCCAGAA 
GAGGAGAGGA TGCAGCAGAG GAGAGAGATG CTACGAGATC CAGAAATGAG AAATAAATTA 
ATTTCTAACC CAACTAATTT TAACCACATA GCACACATGG GCCCAGGGGA TGGAATACAG 
ATCCTAAAAG ACCTGCCTAT GCCAGGCTTC CCTTATCCAT CCCCAAATCA TCACTCCGGT 
CTGATCTCCT CTCCGATCAA CTTTGAGCAC ATCTATCACA TGACTGTTAA TTCTGCTGAA 
AAATTTCTCT CTCCTGATTC CATAAACCCT GCATATTCCC CGTCTCTGCG CTCTGTCCCC 
GGTACACCAA GCTTTATGAC TCTGAGGAAC CCTCGGCCTC AGGAAAGCCG GACCGTGTTC 
AGCGGCTCAG TCAGTATTCC ATCCATCACT AAATCCCGCC CTGAGCCCGG CCGCTCCATG 
AGCGCCAGCA GCGGCCTTTC GGCAAGGTCA TCTGCACAGA ATGGCAGTGC ATTAAAGAGA 
GAATTCTCCG GAGGAAGCTA TAATGCTAAG TGGCAGCCCA TGCCCTCCCC ATCAGAGGGC 
TCCTTGTCTT CCGGAGGCAT GGACCAGGGA AGTGACGCCC CAGCCAGGGA CTATGACGGA 
GAGGACTCGG ATTCTCCGAG GCACTCCACA GCTTCCAACA GCTCCAACCT CAGCAGCCCG 
CCCAGCCCAG TGTCACCTCG GAAGACCAAG AGCCTGTCCC TGGAGAGCAC CGACCGTGGG 
ACCTGGGACC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_022421172.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022276880.1 Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X10, mRNA.

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CloneID	OCe289621
Clone ID Related Accession (Same CDS sequence)	XM_022421172.1
Accession Version	XM_022421172.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase MRCK alpha isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGTG CTTCAGCGTG GAGACGCTGC TGGACATCCT CATCTGCCTT TACGATGAGT 
GCAACAATTC TCCGCTGCGG AGGGAGAAGA ACATTCTTGA ATACCTGGAA TGGGGTTGCT 
GTAGTGAAAC TAAAAAATGC AGATAAAGTA TTTGCCATGA AAATATTGAA TAAATGGGAA 
ATGCTGAAAA GGGCTGAGAC AGCATGTTTT CGTGAAGAAA GGGATGTATT AGTGAATGGA 
GATAATAAAT GGATTACAAC TCTGCATTAT GCTTTCCAGG ATGACAATAA CTTATACTTG 
GTCATGGATT ATTATGTTGG TGGGGATTTG CTTACTCTGC TCAGCAAATT TGAAGATCGA 
TTGCCTGAAG ATATGGCTCG ATTTTACTTG GCCGAGATGG TGATTGCAAT CGATTCTGTT 
CATCAGCTGC ACTATGTGCA TAGGGACATC AAACCAGACA ATATATTGAT GGATATGAAT 
GGACATATTC GATTAGCAGA TTTTGGTTCT TGTCTGAAGC TGATGGAAGA TGGAACGGTC 
CAGTCCTCAG TGGCTGTTGG AACTCCAGAT TATATCTCTC CTGAAATCCT TCAAGCCATG 
GAAGATGGGA AAGGAAGATA CGGGCCTGAA TGTGACTGGT GGTCTCTGGG GGTCTGTATG 
TACGAAATGC TTTATGGAGA AACACCATTT TATGCAGAAT CTCTGGTGGA GACCTATGGA 
AAAATCATGA ATCATAAAGA GAGGTTTCAG TTTCCAGCTC AAGTGACGGA TGTGTCTGAA 
AATGCTAAAG ATCTGATTCG TAGGCTCATT TGCAGCCGAG AACATCGACT TGGCCAAAAT 
GGAATAGAAG ACTTTAAGAA ACACCCATTT TTCAATGGAA TTGATTGGGA TAATATTCGA 
AACTGTGAAG CACCGTATAT TCCAGAAGTT AGTAGCCCAA CAGATACATC GAATTTTGAT 
GTGGATGATG ATTGTTTAAA GAATTCTGAA ACGATGCCCC CACCCACACA TACTGCATTT 
TCTGGCCACC ACCTGCCATT TGTTGGTTTC ACATATACCA GTAGCTGTGT TCTTTCTGAT 
CGGAGCTGTT TACGGGTCAC GGCTGGTCCT GCGTCCCTGG ACCTTGACGT CAGTGTCCAG 
CGGACTCTAG ACAACAACCT GGCCACTGAG GCATATGAGA GGAGAATTAA GCGCCTCGAA 
CAGGAGAAGC TGGAGCTGAG TCGGAAGCTT CAAGAGTCAA CACAGACTGT CCAAGCTCTA 
CAGTATTCAA CTGCTGATGG CCCACTAACA GCAAGCAAAG ATTTAGAAAT AAAAACCTTG 
AAAGAAGAAA TTGAAAACCT AAGGAAACAA ATAAGAGAAT CAAGTCATTT GGAACAACAA 
CTTGAAGAAG CCAACTCTGT GAGGAGAGAG TTAGATGATG CTTTTCGACA AATCAAGGCT 
TATGAAAAAC AAATCAGAAC ACTTCAACAA GAAAGGGAAG AATTAAACAA GGAACTAGTC 
CAGGCTAGTG AGCGATTAAA AAACCAGTCC AAAGAGCTGA AAGACGCACA CTGTCAGAGG 
AAACTGGCCA TGCAGGAATT CATGGAGATC AATGAGCGGC TAACAGAATT GCACACCCAA 
AAACAGAAAC TTGCTCGCCA TGTCCGAGAT AAGGAAGAAG AGGTGGACCT GGTGATGCAA 
AAAGTTGAAA GCTTAAGGCA AGAACTGCGC AGAACAGAGA GAGCCAAAAA AGAGCTGGAA 
GTTCATACAG AAGCTGTGGC TGCTGAAGCA TCTAAAGATA GGAAACTGCG TGAACAGAGT 
GAGCATTATT CTAAGCAACT GGAAAATGAA TTGGAGGGTC TAAAGCAGAA ACAAATTAGT 
TACTCCCCAG GACTGTGCAG CATAGAACAC CAGCAGGAGA TATCCAAACT AAAGACGGAT 
TTGGAAAAGA AAAGTATCTT TTATGAAGAG GAATTGTCTA AAAGAGAAGG AATACATGCA 
AATGAAATTA AAAATCTGAA GAAAGAACTT CATGATTCAG AAGGCCAGCA ACTTGCTCTC 
AACAAGGAAA TTATGATTTT GAAAGACAAA TTAGAGAAAA ACAGAAGAGA GAGTCAAAGT 
GAAAGGGAAG AGTTTGAAAA TGAATTCAAA CAACAATATG AACGAGAAAA AGTATTATTA 
ACAGAAGAAA ATAAAAAACT GACAAGTGAA CTTGATAAGC TCACCACTTT GTATGAAAAT 
GTAAGTATGC GCAACCAGCA GTTAGAAGAA GAGGTGAAAG ACCTAGCAGA CAAGAAAGAA 
TCAGTTGCAC ATTGGGAAGC CCAAATCACA GAAATAATTC AGTGGGTCAG TGATGAAAAG 
GATGCACGAG GGTATCTCCA GGCCTTAGCT TCTAAAATGA CTGAAGAATT GGAAGCTTTG 
AGGAATTCCA GCTTGGGTGC ACGAGCAACA GATATGCCCT GGAAAATGCG TCGTTTTGCA 
AAATTGGATA TGTCAGCTAG ACTGGAGTTG CAGTCAGCTC TGGATGCAGA AATAAGAGCT 
AAACAGGCCA TCCAAGAAGA ACTGAATAAA GTTAAAGCAT CTAACATCAT CACAGAATGT 
AAACTAAAAG ATTCAGAGAA GAAGAACTTG GAGCTCCTCT CAGAAATCGA ACAACTGATA 
AAGGACACTG AAGAGCTTAG ATCTGAAAAG GGTATAGAGC ACCAAGACTC ACAGCATTCT 
TTCTTGGCAT TTTTGAATAC GCCTACCGAT GCTCTGGATC AATTTGAAGA TTCCTTTTCT 
TCTTCCTCAT CTTCACTGAT TGATTTTTTG GATGACCGCT CTCCGTCCTG TACTCCAGCT 
AGCAAAGGCA GACGTACCGA CCCCGTTGAG AGCACATATG TACGGAATCC GAACGTCAAG 
GTTAACGTCC AGTCAAGGTC CACATCTCCT TCTACATCTA GTGAAGCTGA GCCACTTAAG 
ATTGTAGACA GTACTCCTCT TCCAGCTCAC ACACCAACCT TAAGGAAAAA GGGATGTCCT 
GGTTCAACTG GCTTTCCAGC TAAGCGCAAG ACTCATCAGT TTTTTGTCAA ATCTTTCACA 
ACACCTACCA GATGTCACCA GTGCACCTCT CTGATGGTTG GTTTGATAAG ACAAGGCTGT 
TCCTGTGAGG TGTGCGGATT CTCATGTCAT ATAACTTGTG TAAACAAAGC TCCAACTGCT 
TGTCCAGTCC CTCCTGAGCA GACAAAAGGT CCTCTGGGGA TAGATCCACA GAAAGGAATA 
GGAACAGCCT ATGAAGGTCA TGTCAGGATT CCGAAACCAG CTGGAGTAAA GAAAGGATGG 
CAGAGAGCCC TGGCTGTGGT TTGTGACTTT AAACTCTTCC TGTATGATGT CGCTGAAGGA 
AAAGCATCTC AGCCCAGCGT TGTGGTCAGT CAGGTGATTG ACATGAGGGA TGAAGAATTT 
TCTGTGAGTT CAGTGTTGGC TTCTGATGTT ATTCATGCAA GTCGAAAAGA TATACCCTGT 
ATATTCAGAG TCACAGCTTC CCAGCTCTCA GCATCTGACA GCAGGTGTTC GGTCCTAATG 
CTCGCAGAGA GTGAGAGCGA GAGGAACAAG TGGGTGGGCG TGCTCAACGA GCTGCATAAG 
ATTTTGAAGA AAAGCAAATT CAGAGACCGC TCCGTCTATG TTCCCAAGGA GGCTTATGAC 
AGCACTCTGC CCCTCATTAA AACAACCCAG GCAGCTGCAA TCATAGATCA CGAAAGGATA 
GCTCTGGGCA ATGAAGAAGG ATTATTTGTT GTGCACATCA CCAAAGATGA AATTATTAGA 
GTTGGTGATA ATAAGAAGAT ACATCAGATT GAACTCATTC CAAATGATCA GCTTGTTGCT 
GTGATCTCAG GACGAAATCG TCATGTACGA CTTTTTCCTA TATCAGCTTT GGACGGACGA 
GAGACTGATT TTTATAAGCT GGCAGAAACT AAAGGGTGTC AGACCATAAC TTCGGGCAAA 
GTGTGCCATG GAGCTCTCAC ATGCCTGTGT GTGGCTATGA AAAGGCAGGT CCTCTGTTAT 
GAACTATTTC AGAGCAAGAC CCGTCACAGG AAATTTAAGG AAATTCAAGT CCCATATAAC 
GTGCAATGGA TGGCGATCTT CAGTGAACAG CTCTGCGTGG GATTCCAGTC GGGATTCCTA 
AGATACCCGT TGAACGGAGA GGGAAGCCCA TACAGTATGC TCCATTCAAA TGACCACACA 
CTGTCATTTA TTGCCCACCA GCCAATGGAT GCCATCTGCG CAGTTGAGAT CTCCAGTAAA 
GAGTATCTGC TGTGTTTTAA CAGCATTGGG ATATACACTG ACTGCCAGGG CCGGAGATCG 
AGACAACAGG AGTTGATGTG GCCGTCAAAT CCCTCCTCTT GTTGTTACAA CGCACCATAT 
CTCTCAGTGT ATAGTGAGAA TGCAGTTGAT ATCTTCGATG TGAACTCCAT GGAATGGATT 
CAGACTCTCC CACTCAAAAA GGTTCGACCC TTAAACAGTG AAGGATCATT AAACCTTTTA 
GGGCTGGAGA CAATTAGATT AATATATTTC AAAAATAAGA TGGCAGAAGG GGATGAACTG 
GTGGTTCCTG AAACATCGGA CAATAGTCGG AAGCAGATGG TTAGAAACAT CAACAACAAG 
CGACGCTTCT CCTTCAGGGT CCCAGAAGAG GAGAGGATGC AGCAGAGGAG AGAGATGCTA 
CGAGATCCAG AAATGAGAAA TAAATTAATT TCTAACCCAA CTAATTTTAA CCACATAGCA 
CACATGGGCC CAGGGGATGG AATACAGATC CTAAAAGACC TGCCTATGCC AGGCTTCCCT 
TATCCATCCC CAAATCATCA CTCCGGTCTG ATCTCCTCTC CGATCAACTT TGAGCACATC 
TATCACATGA CTGTTAATTC TGCTGAAAAA TTTCTCTCTC CTGATTCCAT AAACCCTGCA 
TATTCCCCGT CTCTGCGCTC TGTCCCCGGT ACACCAAGCT TTATGACTCT GAGGAACCCT 
CGGCCTCAGG AAAGCCGGAC CGTGTTCAGC GGCTCAGTCA GTATTCCATC CATCACTAAA 
TCCCGCCCTG AGCCCGGCCG CTCCATGAGC GCCAGCAGCG GCCTTTCGGC AAGGTCATCT 
GCACAGAATG GCAGTGCATT AAAGAGAGAA TTCTCCGGAG GAAGCTATAA TGCTAAGTGG 
CAGCCCATGC CCTCCCCATC AGAGGGCTCC TTGTCTTCCG GAGGCATGGA CCAGGGAAGT 
GACGCCCCAG CCAGGGACTA TGACGGAGAG GACTCGGATT CTCCGAGGCA CTCCACAGCT 
TCCAACAGCT CCAACCTCAG CAGCCCGCCC AGCCCAGTGT CACCTCGGAA GACCAAGAGC 
CTGTCCCTGG AGAGCACCGA CCGTGGGACC TGGGACCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276880.1
CDS	448..5829
Translation	MGSASAWRRCWTSSSAFTMSATILRCGGRRTFLNTWNGVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPASLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKTLKEEIENLRKQIRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIRTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGLCSIEHQQEISKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLYENVSMRNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGARATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVESTYVRNPNVKVNVQSRSTSPSTSSEAEPLKIVDSTPLPAHTPTLRKKGCPGSTGFPAKRKTHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDVAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASDSRCSVLMLAESESERNKWVGVLNELHKILKKSKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHITKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPISALDGRETDFYKLAETKGCQTITSGKVCHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPSNPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRFSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPNHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPAYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNAKWQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGTWDP

Target ORF information:

RefSeq Version	XM_022421172.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421172.1

ORF Insert Sequence:

ATGGGCAGTG CTTCAGCGTG GAGACGCTGC TGGACATCCT CATCTGCCTT TACGATGAGT 
GCAACAATTC TCCGCTGCGG AGGGAGAAGA ACATTCTTGA ATACCTGGAA TGGGGTTGCT 
GTAGTGAAAC TAAAAAATGC AGATAAAGTA TTTGCCATGA AAATATTGAA TAAATGGGAA 
ATGCTGAAAA GGGCTGAGAC AGCATGTTTT CGTGAAGAAA GGGATGTATT AGTGAATGGA 
GATAATAAAT GGATTACAAC TCTGCATTAT GCTTTCCAGG ATGACAATAA CTTATACTTG 
GTCATGGATT ATTATGTTGG TGGGGATTTG CTTACTCTGC TCAGCAAATT TGAAGATCGA 
TTGCCTGAAG ATATGGCTCG ATTTTACTTG GCCGAGATGG TGATTGCAAT CGATTCTGTT 
CATCAGCTGC ACTATGTGCA TAGGGACATC AAACCAGACA ATATATTGAT GGATATGAAT 
GGACATATTC GATTAGCAGA TTTTGGTTCT TGTCTGAAGC TGATGGAAGA TGGAACGGTC 
CAGTCCTCAG TGGCTGTTGG AACTCCAGAT TATATCTCTC CTGAAATCCT TCAAGCCATG 
GAAGATGGGA AAGGAAGATA CGGGCCTGAA TGTGACTGGT GGTCTCTGGG GGTCTGTATG 
TACGAAATGC TTTATGGAGA AACACCATTT TATGCAGAAT CTCTGGTGGA GACCTATGGA 
AAAATCATGA ATCATAAAGA GAGGTTTCAG TTTCCAGCTC AAGTGACGGA TGTGTCTGAA 
AATGCTAAAG ATCTGATTCG TAGGCTCATT TGCAGCCGAG AACATCGACT TGGCCAAAAT 
GGAATAGAAG ACTTTAAGAA ACACCCATTT TTCAATGGAA TTGATTGGGA TAATATTCGA 
AACTGTGAAG CACCGTATAT TCCAGAAGTT AGTAGCCCAA CAGATACATC GAATTTTGAT 
GTGGATGATG ATTGTTTAAA GAATTCTGAA ACGATGCCCC CACCCACACA TACTGCATTT 
TCTGGCCACC ACCTGCCATT TGTTGGTTTC ACATATACCA GTAGCTGTGT TCTTTCTGAT 
CGGAGCTGTT TACGGGTCAC GGCTGGTCCT GCGTCCCTGG ACCTTGACGT CAGTGTCCAG 
CGGACTCTAG ACAACAACCT GGCCACTGAG GCATATGAGA GGAGAATTAA GCGCCTCGAA 
CAGGAGAAGC TGGAGCTGAG TCGGAAGCTT CAAGAGTCAA CACAGACTGT CCAAGCTCTA 
CAGTATTCAA CTGCTGATGG CCCACTAACA GCAAGCAAAG ATTTAGAAAT AAAAACCTTG 
AAAGAAGAAA TTGAAAACCT AAGGAAACAA ATAAGAGAAT CAAGTCATTT GGAACAACAA 
CTTGAAGAAG CCAACTCTGT GAGGAGAGAG TTAGATGATG CTTTTCGACA AATCAAGGCT 
TATGAAAAAC AAATCAGAAC ACTTCAACAA GAAAGGGAAG AATTAAACAA GGAACTAGTC 
CAGGCTAGTG AGCGATTAAA AAACCAGTCC AAAGAGCTGA AAGACGCACA CTGTCAGAGG 
AAACTGGCCA TGCAGGAATT CATGGAGATC AATGAGCGGC TAACAGAATT GCACACCCAA 
AAACAGAAAC TTGCTCGCCA TGTCCGAGAT AAGGAAGAAG AGGTGGACCT GGTGATGCAA 
AAAGTTGAAA GCTTAAGGCA AGAACTGCGC AGAACAGAGA GAGCCAAAAA AGAGCTGGAA 
GTTCATACAG AAGCTGTGGC TGCTGAAGCA TCTAAAGATA GGAAACTGCG TGAACAGAGT 
GAGCATTATT CTAAGCAACT GGAAAATGAA TTGGAGGGTC TAAAGCAGAA ACAAATTAGT 
TACTCCCCAG GACTGTGCAG CATAGAACAC CAGCAGGAGA TATCCAAACT AAAGACGGAT 
TTGGAAAAGA AAAGTATCTT TTATGAAGAG GAATTGTCTA AAAGAGAAGG AATACATGCA 
AATGAAATTA AAAATCTGAA GAAAGAACTT CATGATTCAG AAGGCCAGCA ACTTGCTCTC 
AACAAGGAAA TTATGATTTT GAAAGACAAA TTAGAGAAAA ACAGAAGAGA GAGTCAAAGT 
GAAAGGGAAG AGTTTGAAAA TGAATTCAAA CAACAATATG AACGAGAAAA AGTATTATTA 
ACAGAAGAAA ATAAAAAACT GACAAGTGAA CTTGATAAGC TCACCACTTT GTATGAAAAT 
GTAAGTATGC GCAACCAGCA GTTAGAAGAA GAGGTGAAAG ACCTAGCAGA CAAGAAAGAA 
TCAGTTGCAC ATTGGGAAGC CCAAATCACA GAAATAATTC AGTGGGTCAG TGATGAAAAG 
GATGCACGAG GGTATCTCCA GGCCTTAGCT TCTAAAATGA CTGAAGAATT GGAAGCTTTG 
AGGAATTCCA GCTTGGGTGC ACGAGCAACA GATATGCCCT GGAAAATGCG TCGTTTTGCA 
AAATTGGATA TGTCAGCTAG ACTGGAGTTG CAGTCAGCTC TGGATGCAGA AATAAGAGCT 
AAACAGGCCA TCCAAGAAGA ACTGAATAAA GTTAAAGCAT CTAACATCAT CACAGAATGT 
AAACTAAAAG ATTCAGAGAA GAAGAACTTG GAGCTCCTCT CAGAAATCGA ACAACTGATA 
AAGGACACTG AAGAGCTTAG ATCTGAAAAG GGTATAGAGC ACCAAGACTC ACAGCATTCT 
TTCTTGGCAT TTTTGAATAC GCCTACCGAT GCTCTGGATC AATTTGAAGA TTCCTTTTCT 
TCTTCCTCAT CTTCACTGAT TGATTTTTTG GATGACCGCT CTCCGTCCTG TACTCCAGCT 
AGCAAAGGCA GACGTACCGA CCCCGTTGAG AGCACATATG TACGGAATCC GAACGTCAAG 
GTTAACGTCC AGTCAAGGTC CACATCTCCT TCTACATCTA GTGAAGCTGA GCCACTTAAG 
ATTGTAGACA GTACTCCTCT TCCAGCTCAC ACACCAACCT TAAGGAAAAA GGGATGTCCT 
GGTTCAACTG GCTTTCCAGC TAAGCGCAAG ACTCATCAGT TTTTTGTCAA ATCTTTCACA 
ACACCTACCA GATGTCACCA GTGCACCTCT CTGATGGTTG GTTTGATAAG ACAAGGCTGT 
TCCTGTGAGG TGTGCGGATT CTCATGTCAT ATAACTTGTG TAAACAAAGC TCCAACTGCT 
TGTCCAGTCC CTCCTGAGCA GACAAAAGGT CCTCTGGGGA TAGATCCACA GAAAGGAATA 
GGAACAGCCT ATGAAGGTCA TGTCAGGATT CCGAAACCAG CTGGAGTAAA GAAAGGATGG 
CAGAGAGCCC TGGCTGTGGT TTGTGACTTT AAACTCTTCC TGTATGATGT CGCTGAAGGA 
AAAGCATCTC AGCCCAGCGT TGTGGTCAGT CAGGTGATTG ACATGAGGGA TGAAGAATTT 
TCTGTGAGTT CAGTGTTGGC TTCTGATGTT ATTCATGCAA GTCGAAAAGA TATACCCTGT 
ATATTCAGAG TCACAGCTTC CCAGCTCTCA GCATCTGACA GCAGGTGTTC GGTCCTAATG 
CTCGCAGAGA GTGAGAGCGA GAGGAACAAG TGGGTGGGCG TGCTCAACGA GCTGCATAAG 
ATTTTGAAGA AAAGCAAATT CAGAGACCGC TCCGTCTATG TTCCCAAGGA GGCTTATGAC 
AGCACTCTGC CCCTCATTAA AACAACCCAG GCAGCTGCAA TCATAGATCA CGAAAGGATA 
GCTCTGGGCA ATGAAGAAGG ATTATTTGTT GTGCACATCA CCAAAGATGA AATTATTAGA 
GTTGGTGATA ATAAGAAGAT ACATCAGATT GAACTCATTC CAAATGATCA GCTTGTTGCT 
GTGATCTCAG GACGAAATCG TCATGTACGA CTTTTTCCTA TATCAGCTTT GGACGGACGA 
GAGACTGATT TTTATAAGCT GGCAGAAACT AAAGGGTGTC AGACCATAAC TTCGGGCAAA 
GTGTGCCATG GAGCTCTCAC ATGCCTGTGT GTGGCTATGA AAAGGCAGGT CCTCTGTTAT 
GAACTATTTC AGAGCAAGAC CCGTCACAGG AAATTTAAGG AAATTCAAGT CCCATATAAC 
GTGCAATGGA TGGCGATCTT CAGTGAACAG CTCTGCGTGG GATTCCAGTC GGGATTCCTA 
AGATACCCGT TGAACGGAGA GGGAAGCCCA TACAGTATGC TCCATTCAAA TGACCACACA 
CTGTCATTTA TTGCCCACCA GCCAATGGAT GCCATCTGCG CAGTTGAGAT CTCCAGTAAA 
GAGTATCTGC TGTGTTTTAA CAGCATTGGG ATATACACTG ACTGCCAGGG CCGGAGATCG 
AGACAACAGG AGTTGATGTG GCCGTCAAAT CCCTCCTCTT GTTGTTACAA CGCACCATAT 
CTCTCAGTGT ATAGTGAGAA TGCAGTTGAT ATCTTCGATG TGAACTCCAT GGAATGGATT 
CAGACTCTCC CACTCAAAAA GGTTCGACCC TTAAACAGTG AAGGATCATT AAACCTTTTA 
GGGCTGGAGA CAATTAGATT AATATATTTC AAAAATAAGA TGGCAGAAGG GGATGAACTG 
GTGGTTCCTG AAACATCGGA CAATAGTCGG AAGCAGATGG TTAGAAACAT CAACAACAAG 
CGACGCTTCT CCTTCAGGGT CCCAGAAGAG GAGAGGATGC AGCAGAGGAG AGAGATGCTA 
CGAGATCCAG AAATGAGAAA TAAATTAATT TCTAACCCAA CTAATTTTAA CCACATAGCA 
CACATGGGCC CAGGGGATGG AATACAGATC CTAAAAGACC TGCCTATGCC AGGCTTCCCT 
TATCCATCCC CAAATCATCA CTCCGGTCTG ATCTCCTCTC CGATCAACTT TGAGCACATC 
TATCACATGA CTGTTAATTC TGCTGAAAAA TTTCTCTCTC CTGATTCCAT AAACCCTGCA 
TATTCCCCGT CTCTGCGCTC TGTCCCCGGT ACACCAAGCT TTATGACTCT GAGGAACCCT 
CGGCCTCAGG AAAGCCGGAC CGTGTTCAGC GGCTCAGTCA GTATTCCATC CATCACTAAA 
TCCCGCCCTG AGCCCGGCCG CTCCATGAGC GCCAGCAGCG GCCTTTCGGC AAGGTCATCT 
GCACAGAATG GCAGTGCATT AAAGAGAGAA TTCTCCGGAG GAAGCTATAA TGCTAAGTGG 
CAGCCCATGC CCTCCCCATC AGAGGGCTCC TTGTCTTCCG GAGGCATGGA CCAGGGAAGT 
GACGCCCCAG CCAGGGACTA TGACGGAGAG GACTCGGATT CTCCGAGGCA CTCCACAGCT 
TCCAACAGCT CCAACCTCAG CAGCCCGCCC AGCCCAGTGT CACCTCGGAA GACCAAGAGC 
CTGTCCCTGG AGAGCACCGA CCGTGGGACC TGGGACCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_022421174.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022276882.1 Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X14, mRNA.

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CloneID	OCe289622
Clone ID Related Accession (Same CDS sequence)	XM_022421174.1
Accession Version	XM_022421174.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase MRCK alpha isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGCTG GAAGTTCATA CAGAAGCTGT GGCTGCTGAA 
GCATCTAAAG ATAGGAAACT GCGTGAACAG AGTGAGCATT ATTCTAAGCA ACTGGAAAAT 
GAATTGGAGG GTCTAAAGAA ACAAATTAGT TACTCCCCAG GACTGTGCAG CATAGAACAC 
CAGCAGGAGA TATCCAAACT AAAGACGGAT TTGGAAAAGA AAAGTATCTT TTATGAAGAG 
GAATTGTCTA AAAGAGAAGG AATACATGCA AATGAAATTA AAAATCTGAA GAAAGAACTT 
CATGATTCAG AAGGCCAGCA ACTTGCTCTC AACAAGGAAA TTATGATTTT GAAAGACAAA 
TTAGAGAAAA ACAGAAGAGA GAGTCAAAGT GAAAGGGAAG AGTTTGAAAA TGAATTCAAA 
CAACAATATG AACGAGAAAA AGTATTATTA ACAGAAGAAA ATAAAAAACT GACAAGTGAA 
CTTGATAAGC TCACCACTTT GTATGAAAAT GTAAGTATGC GCAACCAGCA GTTAGAAGAA 
GAGGTGAAAG ACCTAGCAGA CAAGAAAGAA TCAGTTGCAC ATTGGGAAGC CCAAATCACA 
GAAATAATTC AGTGGGTCAG TGATGAAAAG GATGCACGAG GGTATCTCCA GGCCTTAGCT 
TCTAAAATGA CTGAAGAATT GGAAGCTTTG AGGAATTCCA GCTTGGGTGC ACGAGCAACA 
GATATGCCCT GGAAAATGCG TCGTTTTGCA AAATTGGATA TGTCAGCTAG ACTGGAGTTG 
CAGTCAGCTC TGGATGCAGA AATAAGAGCT AAACAGGCCA TCCAAGAAGA ACTGAATAAA 
GTTAAAGCAT CTAACATCAT CACAGAATGT AAACTAAAAG ATTCAGAGAA GAAGAACTTG 
GAGCTCCTCT CAGAAATCGA ACAACTGATA AAGGACACTG AAGAGCTTAG ATCTGAAAAG 
GGTATAGAGC ACCAAGACTC ACAGCATTCT TTCTTGGCAT TTTTGAATAC GCCTACCGAT 
GCTCTGGATC AATTTGAAGA TTCCTTTTCT TCTTCCTCAT CTTCACTGAT TGATTTTTTG 
GATGACCGCT CTCCGTCCTG TACTCCAGCT AGCAAAGGCA GACGTACCGA CCCCGTTGAG 
AGCACATATG TACGGAATCC GAACGTCAAG GTTAACGTCC AGTCAAGGTC CACATCTCCT 
TCTACATCTA GTGAAGCTGA GCCACTTAAG ATTGTAGACA GTACTCCTCT TCCAGCTCAC 
ACACCAACCT TAAGGAAAAA GGGATGTCCT GGTTCAACTG GCTTTCCAGC TAAGCGCAAG 
ACTCATCAGT TTTTTGTCAA ATCTTTCACA ACACCTACCA GATGTCACCA GTGCACCTCT 
CTGATGGTTG GTTTGATAAG ACAAGGCTGT TCCTGTGAGG TGTGCGGATT CTCATGTCAT 
ATAACTTGTG TAAACAAAGC TCCAACTGCT TGTCCAGTCC CTCCTGAGCA GACAAAAGGT 
CCTCTGGGGA TAGATCCACA GAAAGGAATA GGAACAGCCT ATGAAGGTCA TGTCAGGATT 
CCGAAACCAG CTGGAGTAAA GAAAGGATGG CAGAGAGCCC TGGCTGTGGT TTGTGACTTT 
AAACTCTTCC TGTATGATGT CGCTGAAGGA AAAGCATCTC AGCCCAGCGT TGTGGTCAGT 
CAGGTGATTG ACATGAGGGA TGAAGAATTT TCTGTGAGTT CAGTGTTGGC TTCTGATGTT 
ATTCATGCAA GTCGAAAAGA TATACCCTGT ATATTCAGAG TCACAGCTTC CCAGCTCTCA 
GCATCTGACA GCAGGTGTTC GGTCCTAATG CTCGCAGAGA GTGAGAGCGA GAGGAACAAG 
TGGGTGGGCG TGCTCAACGA GCTGCATAAG ATTTTGAAGA AAAGCAAATT CAGAGACCGC 
TCCGTCTATG TTCCCAAGGA GGCTTATGAC AGCACTCTGC CCCTCATTAA AACAACCCAG 
GCAGCTGCAA TCATAGATCA CGAAAGGATA GCTCTGGGCA ATGAAGAAGG ATTATTTGTT 
GTGCACATCA CCAAAGATGA AATTATTAGA GTTGGTGATA ATAAGAAGAT ACATCAGATT 
GAACTCATTC CAAATGATCA GCTTGTTGCT GTGATCTCAG GACGAAATCG TCATGTACGA 
CTTTTTCCTA TATCAGCTTT GGACGGACGA GAGACTGATT TTTATAAGCT GGCAGAAACT 
AAAGGGTGTC AGACCATAAC TTCGGGCAAA GTGTGCCATG GAGCTCTCAC ATGCCTGTGT 
GTGGCTATGA AAAGGCAGGT CCTCTGTTAT GAACTATTTC AGAGCAAGAC CCGTCACAGG 
AAATTTAAGG AAATTCAAGT CCCATATAAC GTGCAATGGA TGGCGATCTT CAGTGAACAG 
CTCTGCGTGG GATTCCAGTC GGGATTCCTA AGATACCCGT TGAACGGAGA GGGAAGCCCA 
TACAGTATGC TCCATTCAAA TGACCACACA CTGTCATTTA TTGCCCACCA GCCAATGGAT 
GCCATCTGCG CAGTTGAGAT CTCCAGTAAA GAGTATCTGC TGTGTTTTAA CAGCATTGGG 
ATATACACTG ACTGCCAGGG CCGGAGATCG AGACAACAGG AGTTGATGTG GCCGTCAAAT 
CCCTCCTCTT GTTGTTACAA CGCACCATAT CTCTCAGTGT ATAGTGAGAA TGCAGTTGAT 
ATCTTCGATG TGAACTCCAT GGAATGGATT CAGACTCTCC CACTCAAAAA GGTTCGACCC 
TTAAACAGTG AAGGATCATT AAACCTTTTA GGGCTGGAGA CAATTAGATT AATATATTTC 
AAAAATAAGA TGGCAGAAGG GGATGAACTG GTGGTTCCTG AAACATCGGA CAATAGTCGG 
AAGCAGATGG TTAGAAACAT CAACAACAAG CGACGCTTCT CCTTCAGGGT CCCAGAAGAG 
GAGAGGATGC AGCAGAGGAG AGAGATGCTA CGAGATCCAG AAATGAGAAA TAAATTAATT 
TCTAACCCAA CTAATTTTAA CCACATAGCA CACATGGGCC CAGGGGATGG AATACAGATC 
CTAAAAGACC TGCCTATGCC AGGCTTCCCT TATCCATCCC CAAATCATCA CTCCGGTCTG 
ATCTCCTCTC CGATCAACTT TGAGCACATC TATCACATGA CTGTTAATTC TGCTGAAAAA 
TTTCTCTCTC CTGATTCCAT AAACCCTGCA TATTCCCCGT CTCTGCGCTC TGTCCCCGGT 
ACACCAAGCT TTATGACTCT GAGGAACCCT CGGCCTCAGG AAAGCCGGAC CGTGTTCAGC 
GGCTCAGTCA GTATTCCATC CATCACTAAA TCCCGCCCTG AGCCCGGCCG CTCCATGAGC 
GCCAGCAGCG GCCTTTCGGC AAGGTCATCT GCACAGAATG GCAGTGCATT AAAGAGAGAA 
TTCTCCGGAG GAAGCTATAA TGCTAAGTGG CAGCCCATGC CCTCCCCATC AGAGGGCTCC 
TTGTCTTCCG GAGGCATGGA CCAGGGAAGT GACGCCCCAG CCAGGGACTA TGACGGAGAG 
GACTCGGATT CTCCGAGGCA CTCCACAGCT TCCAACAGCT CCAACCTCAG CAGCCCGCCC 
AGCCCAGTGT CACCTCGGAA GACCAAGAGC CTGTCCCTGG AGAGCACCGA CCGTGGGACC 
TGGGACCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276882.1
CDS	384..5675
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPASLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKTLKEEIENLRKQIRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIRTLQQEREELNKLEVHTEAVAAEASKDRKLREQSEHYSKQLENELEGLKKQISYSPGLCSIEHQQEISKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLYENVSMRNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGARATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVESTYVRNPNVKVNVQSRSTSPSTSSEAEPLKIVDSTPLPAHTPTLRKKGCPGSTGFPAKRKTHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDVAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASDSRCSVLMLAESESERNKWVGVLNELHKILKKSKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHITKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPISALDGRETDFYKLAETKGCQTITSGKVCHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPSNPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRFSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPNHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPAYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNAKWQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGTWDP

Target ORF information:

RefSeq Version	XM_022421174.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421174.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGCTG GAAGTTCATA CAGAAGCTGT GGCTGCTGAA 
GCATCTAAAG ATAGGAAACT GCGTGAACAG AGTGAGCATT ATTCTAAGCA ACTGGAAAAT 
GAATTGGAGG GTCTAAAGAA ACAAATTAGT TACTCCCCAG GACTGTGCAG CATAGAACAC 
CAGCAGGAGA TATCCAAACT AAAGACGGAT TTGGAAAAGA AAAGTATCTT TTATGAAGAG 
GAATTGTCTA AAAGAGAAGG AATACATGCA AATGAAATTA AAAATCTGAA GAAAGAACTT 
CATGATTCAG AAGGCCAGCA ACTTGCTCTC AACAAGGAAA TTATGATTTT GAAAGACAAA 
TTAGAGAAAA ACAGAAGAGA GAGTCAAAGT GAAAGGGAAG AGTTTGAAAA TGAATTCAAA 
CAACAATATG AACGAGAAAA AGTATTATTA ACAGAAGAAA ATAAAAAACT GACAAGTGAA 
CTTGATAAGC TCACCACTTT GTATGAAAAT GTAAGTATGC GCAACCAGCA GTTAGAAGAA 
GAGGTGAAAG ACCTAGCAGA CAAGAAAGAA TCAGTTGCAC ATTGGGAAGC CCAAATCACA 
GAAATAATTC AGTGGGTCAG TGATGAAAAG GATGCACGAG GGTATCTCCA GGCCTTAGCT 
TCTAAAATGA CTGAAGAATT GGAAGCTTTG AGGAATTCCA GCTTGGGTGC ACGAGCAACA 
GATATGCCCT GGAAAATGCG TCGTTTTGCA AAATTGGATA TGTCAGCTAG ACTGGAGTTG 
CAGTCAGCTC TGGATGCAGA AATAAGAGCT AAACAGGCCA TCCAAGAAGA ACTGAATAAA 
GTTAAAGCAT CTAACATCAT CACAGAATGT AAACTAAAAG ATTCAGAGAA GAAGAACTTG 
GAGCTCCTCT CAGAAATCGA ACAACTGATA AAGGACACTG AAGAGCTTAG ATCTGAAAAG 
GGTATAGAGC ACCAAGACTC ACAGCATTCT TTCTTGGCAT TTTTGAATAC GCCTACCGAT 
GCTCTGGATC AATTTGAAGA TTCCTTTTCT TCTTCCTCAT CTTCACTGAT TGATTTTTTG 
GATGACCGCT CTCCGTCCTG TACTCCAGCT AGCAAAGGCA GACGTACCGA CCCCGTTGAG 
AGCACATATG TACGGAATCC GAACGTCAAG GTTAACGTCC AGTCAAGGTC CACATCTCCT 
TCTACATCTA GTGAAGCTGA GCCACTTAAG ATTGTAGACA GTACTCCTCT TCCAGCTCAC 
ACACCAACCT TAAGGAAAAA GGGATGTCCT GGTTCAACTG GCTTTCCAGC TAAGCGCAAG 
ACTCATCAGT TTTTTGTCAA ATCTTTCACA ACACCTACCA GATGTCACCA GTGCACCTCT 
CTGATGGTTG GTTTGATAAG ACAAGGCTGT TCCTGTGAGG TGTGCGGATT CTCATGTCAT 
ATAACTTGTG TAAACAAAGC TCCAACTGCT TGTCCAGTCC CTCCTGAGCA GACAAAAGGT 
CCTCTGGGGA TAGATCCACA GAAAGGAATA GGAACAGCCT ATGAAGGTCA TGTCAGGATT 
CCGAAACCAG CTGGAGTAAA GAAAGGATGG CAGAGAGCCC TGGCTGTGGT TTGTGACTTT 
AAACTCTTCC TGTATGATGT CGCTGAAGGA AAAGCATCTC AGCCCAGCGT TGTGGTCAGT 
CAGGTGATTG ACATGAGGGA TGAAGAATTT TCTGTGAGTT CAGTGTTGGC TTCTGATGTT 
ATTCATGCAA GTCGAAAAGA TATACCCTGT ATATTCAGAG TCACAGCTTC CCAGCTCTCA 
GCATCTGACA GCAGGTGTTC GGTCCTAATG CTCGCAGAGA GTGAGAGCGA GAGGAACAAG 
TGGGTGGGCG TGCTCAACGA GCTGCATAAG ATTTTGAAGA AAAGCAAATT CAGAGACCGC 
TCCGTCTATG TTCCCAAGGA GGCTTATGAC AGCACTCTGC CCCTCATTAA AACAACCCAG 
GCAGCTGCAA TCATAGATCA CGAAAGGATA GCTCTGGGCA ATGAAGAAGG ATTATTTGTT 
GTGCACATCA CCAAAGATGA AATTATTAGA GTTGGTGATA ATAAGAAGAT ACATCAGATT 
GAACTCATTC CAAATGATCA GCTTGTTGCT GTGATCTCAG GACGAAATCG TCATGTACGA 
CTTTTTCCTA TATCAGCTTT GGACGGACGA GAGACTGATT TTTATAAGCT GGCAGAAACT 
AAAGGGTGTC AGACCATAAC TTCGGGCAAA GTGTGCCATG GAGCTCTCAC ATGCCTGTGT 
GTGGCTATGA AAAGGCAGGT CCTCTGTTAT GAACTATTTC AGAGCAAGAC CCGTCACAGG 
AAATTTAAGG AAATTCAAGT CCCATATAAC GTGCAATGGA TGGCGATCTT CAGTGAACAG 
CTCTGCGTGG GATTCCAGTC GGGATTCCTA AGATACCCGT TGAACGGAGA GGGAAGCCCA 
TACAGTATGC TCCATTCAAA TGACCACACA CTGTCATTTA TTGCCCACCA GCCAATGGAT 
GCCATCTGCG CAGTTGAGAT CTCCAGTAAA GAGTATCTGC TGTGTTTTAA CAGCATTGGG 
ATATACACTG ACTGCCAGGG CCGGAGATCG AGACAACAGG AGTTGATGTG GCCGTCAAAT 
CCCTCCTCTT GTTGTTACAA CGCACCATAT CTCTCAGTGT ATAGTGAGAA TGCAGTTGAT 
ATCTTCGATG TGAACTCCAT GGAATGGATT CAGACTCTCC CACTCAAAAA GGTTCGACCC 
TTAAACAGTG AAGGATCATT AAACCTTTTA GGGCTGGAGA CAATTAGATT AATATATTTC 
AAAAATAAGA TGGCAGAAGG GGATGAACTG GTGGTTCCTG AAACATCGGA CAATAGTCGG 
AAGCAGATGG TTAGAAACAT CAACAACAAG CGACGCTTCT CCTTCAGGGT CCCAGAAGAG 
GAGAGGATGC AGCAGAGGAG AGAGATGCTA CGAGATCCAG AAATGAGAAA TAAATTAATT 
TCTAACCCAA CTAATTTTAA CCACATAGCA CACATGGGCC CAGGGGATGG AATACAGATC 
CTAAAAGACC TGCCTATGCC AGGCTTCCCT TATCCATCCC CAAATCATCA CTCCGGTCTG 
ATCTCCTCTC CGATCAACTT TGAGCACATC TATCACATGA CTGTTAATTC TGCTGAAAAA 
TTTCTCTCTC CTGATTCCAT AAACCCTGCA TATTCCCCGT CTCTGCGCTC TGTCCCCGGT 
ACACCAAGCT TTATGACTCT GAGGAACCCT CGGCCTCAGG AAAGCCGGAC CGTGTTCAGC 
GGCTCAGTCA GTATTCCATC CATCACTAAA TCCCGCCCTG AGCCCGGCCG CTCCATGAGC 
GCCAGCAGCG GCCTTTCGGC AAGGTCATCT GCACAGAATG GCAGTGCATT AAAGAGAGAA 
TTCTCCGGAG GAAGCTATAA TGCTAAGTGG CAGCCCATGC CCTCCCCATC AGAGGGCTCC 
TTGTCTTCCG GAGGCATGGA CCAGGGAAGT GACGCCCCAG CCAGGGACTA TGACGGAGAG 
GACTCGGATT CTCCGAGGCA CTCCACAGCT TCCAACAGCT CCAACCTCAG CAGCCCGCCC 
AGCCCAGTGT CACCTCGGAA GACCAAGAGC CTGTCCCTGG AGAGCACCGA CCGTGGGACC 
TGGGACCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_005622614.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622671.1 Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe09378
Clone ID Related Accession (Same CDS sequence)	XM_005622614.1
Accession Version	XM_005622614.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase MRCK alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC GTCCTGTACT CCAGCTAGCA AAGGCAGACG TACCGACCCC 
GTTGAGAGCA CATATGTACG GAATCCGAAC GTCAAGGTTA ACGTCCAGTC AAGGTCCACA 
TCTCCTTCTA CATCTAGTGA AGCTGAGCCA CTTAAGATTG TAGACAGTAC TCCTCTTCCA 
GCTCACACAC CAACCTTAAG GAAAAAGGGA TGTCCTGGTT CAACTGGCTT TCCAGCTAAG 
CGCAAGACTC ATCAGTTTTT TGTCAAATCT TTCACAACAC CTACCAGATG TCACCAGTGC 
ACCTCTCTGA TGGTTGGTTT GATAAGACAA GGCTGTTCCT GTGAGGTGTG CGGATTCTCA 
TGTCATATAA CTTGTGTAAA CAAAGCTCCA ACTGCTTGTC CAGTCCCTCC TGAGCAGACA 
AAAGGTCCTC TGGGGATAGA TCCACAGAAA GGAATAGGAA CAGCCTATGA AGGTCATGTC 
AGGATTCCGA AACCAGCTGG AGTAAAGAAA GGATGGCAGA GAGCCCTGGC TGTGGTTTGT 
GACTTTAAAC TCTTCCTGTA TGATGTCGCT GAAGGAAAAG CATCTCAGCC CAGCGTTGTG 
GTCAGTCAGG TGATTGACAT GAGGGATGAA GAATTTTCTG TGAGTTCAGT GTTGGCTTCT 
GATGTTATTC ATGCAAGTCG AAAAGATATA CCCTGTATAT TCAGAGTCAC AGCTTCCCAG 
CTCTCAGCAT CTGACAGCAG GTGTTCGGTC CTAATGCTCG CAGAGAGTGA GAGCGAGAGG 
AACAAGTGGG TGGGCGTGCT CAACGAGCTG CATAAGATTT TGAAGAAAAG CAAATTCAGA 
GACCGCTCCG TCTATGTTCC CAAGGAGGCT TATGACAGCA CTCTGCCCCT CATTAAAACA 
ACCCAGGCAG CTGCAATCAT AGATCACGAA AGGATAGCTC TGGGCAATGA AGAAGGATTA 
TTTGTTGTGC ACATCACCAA AGATGAAATT ATTAGAGTTG GTGATAATAA GAAGATACAT 
CAGATTGAAC TCATTCCAAA TGATCAGCTT GTTGCTGTGA TCTCAGGACG AAATCGTCAT 
GTACGACTTT TTCCTATATC AGCTTTGGAC GGACGAGAGA CTGATTTTTA TAAGCTGGCA 
GAAACTAAAG GGTGTCAGAC CATAACTTCG GGCAAAGTGT GCCATGGAGC TCTCACATGC 
CTGTGTGTGG CTATGAAAAG GCAGGTCCTC TGTTATGAAC TATTTCAGAG CAAGACCCGT 
CACAGGAAAT TTAAGGAAAT TCAAGTCCCA TATAACGTGC AATGGATGGC GATCTTCAGT 
GAACAGCTCT GCGTGGGATT CCAGTCGGGA TTCCTAAGAT ACCCGTTGAA CGGAGAGGGA 
AGCCCATACA GTATGCTCCA TTCAAATGAC CACACACTGT CATTTATTGC CCACCAGCCA 
ATGGATGCCA TCTGCGCAGT TGAGATCTCC AGTAAAGAGT ATCTGCTGTG TTTTAACAGC 
ATTGGGATAT ACACTGACTG CCAGGGCCGG AGATCGAGAC AACAGGAGTT GATGTGGCCG 
TCAAATCCCT CCTCTTGTTG TTACAACGCA CCATATCTCT CAGTGTATAG TGAGAATGCA 
GTTGATATCT TCGATGTGAA CTCCATGGAA TGGATTCAGA CTCTCCCACT CAAAAAGGTT 
CGACCCTTAA ACAGTGAAGG ATCATTAAAC CTTTTAGGGC TGGAGACAAT TAGATTAATA 
TATTTCAAAA ATAAGATGGC AGAAGGGGAT GAACTGGTGG TTCCTGAAAC ATCGGACAAT 
AGTCGGAAGC AGATGGTTAG AAACATCAAC AACAAGCGAC GCTTCTCCTT CAGGGTCCCA 
GAAGAGGAGA GGATGCAGCA GAGGAGAGAG ATGCTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ACCCAACTAA TTTTAACCAC ATAGCACACA TGGGCCCAGG GGATGGAATA 
CAGATCCTAA AAGACCTGCC TATGCCAGGC TTCCCTTATC CATCCCCAAA TCATCACTCC 
GGTCTGATCT CCTCTCCGAT CAACTTTGAG CACATCTATC ACATGACTGT TAATTCTGCT 
GAAAAATTTC TCTCTCCTGA TTCCATAAAC CCTGCATATT CCCCGTCTCT GCGCTCTGTC 
CCCGGTACAC CAAGCTTTAT GACTCTGAGG AACCCTCGGC CTCAGGAAAG CCGGACCGTG 
TTCAGCGGCT CAGTCAGTAT TCCATCCATC ACTAAATCCC GCCCTGAGCC CGGCCGCTCC 
ATGAGCGCCA GCAGCGGCCT TTCGGCAAGG TCATCTGCAC AGAATGGCAG TGCATTAAAG 
AGAGAATTCT CCGGAGGAAG CTATAATGCT AAGTGGCAGC CCATGCCCTC CCCATCAGAG 
GGCTCCTTGT CTTCCGGAGG CATGGACCAG GGAAGTGACG CCCCAGCCAG GGACTATGAC 
GGAGAGGACT CGGATTCTCC GAGGCACTCC ACAGCTTCCA ACAGCTCCAA CCTCAGCAGC 
CCGCCCAGCC CAGTGTCACC TCGGAAGACC AAGAGCCTGT CCCTGGAGAG CACCGACCGT 
GGGACCTGGG ACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622671.1
CDS	384..5921
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPASLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKTLKEEIENLRKQIRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIRTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGLCSIEHQQEISKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLYENVSMRNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGARATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVESTYVRNPNVKVNVQSRSTSPSTSSEAEPLKIVDSTPLPAHTPTLRKKGCPGSTGFPAKRKTHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDVAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASDSRCSVLMLAESESERNKWVGVLNELHKILKKSKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHITKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPISALDGRETDFYKLAETKGCQTITSGKVCHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPSNPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRFSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPNHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPAYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNAKWQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGTWDP

Target ORF information:

RefSeq Version	XM_005622614.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622614.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC GTCCTGTACT CCAGCTAGCA AAGGCAGACG TACCGACCCC 
GTTGAGAGCA CATATGTACG GAATCCGAAC GTCAAGGTTA ACGTCCAGTC AAGGTCCACA 
TCTCCTTCTA CATCTAGTGA AGCTGAGCCA CTTAAGATTG TAGACAGTAC TCCTCTTCCA 
GCTCACACAC CAACCTTAAG GAAAAAGGGA TGTCCTGGTT CAACTGGCTT TCCAGCTAAG 
CGCAAGACTC ATCAGTTTTT TGTCAAATCT TTCACAACAC CTACCAGATG TCACCAGTGC 
ACCTCTCTGA TGGTTGGTTT GATAAGACAA GGCTGTTCCT GTGAGGTGTG CGGATTCTCA 
TGTCATATAA CTTGTGTAAA CAAAGCTCCA ACTGCTTGTC CAGTCCCTCC TGAGCAGACA 
AAAGGTCCTC TGGGGATAGA TCCACAGAAA GGAATAGGAA CAGCCTATGA AGGTCATGTC 
AGGATTCCGA AACCAGCTGG AGTAAAGAAA GGATGGCAGA GAGCCCTGGC TGTGGTTTGT 
GACTTTAAAC TCTTCCTGTA TGATGTCGCT GAAGGAAAAG CATCTCAGCC CAGCGTTGTG 
GTCAGTCAGG TGATTGACAT GAGGGATGAA GAATTTTCTG TGAGTTCAGT GTTGGCTTCT 
GATGTTATTC ATGCAAGTCG AAAAGATATA CCCTGTATAT TCAGAGTCAC AGCTTCCCAG 
CTCTCAGCAT CTGACAGCAG GTGTTCGGTC CTAATGCTCG CAGAGAGTGA GAGCGAGAGG 
AACAAGTGGG TGGGCGTGCT CAACGAGCTG CATAAGATTT TGAAGAAAAG CAAATTCAGA 
GACCGCTCCG TCTATGTTCC CAAGGAGGCT TATGACAGCA CTCTGCCCCT CATTAAAACA 
ACCCAGGCAG CTGCAATCAT AGATCACGAA AGGATAGCTC TGGGCAATGA AGAAGGATTA 
TTTGTTGTGC ACATCACCAA AGATGAAATT ATTAGAGTTG GTGATAATAA GAAGATACAT 
CAGATTGAAC TCATTCCAAA TGATCAGCTT GTTGCTGTGA TCTCAGGACG AAATCGTCAT 
GTACGACTTT TTCCTATATC AGCTTTGGAC GGACGAGAGA CTGATTTTTA TAAGCTGGCA 
GAAACTAAAG GGTGTCAGAC CATAACTTCG GGCAAAGTGT GCCATGGAGC TCTCACATGC 
CTGTGTGTGG CTATGAAAAG GCAGGTCCTC TGTTATGAAC TATTTCAGAG CAAGACCCGT 
CACAGGAAAT TTAAGGAAAT TCAAGTCCCA TATAACGTGC AATGGATGGC GATCTTCAGT 
GAACAGCTCT GCGTGGGATT CCAGTCGGGA TTCCTAAGAT ACCCGTTGAA CGGAGAGGGA 
AGCCCATACA GTATGCTCCA TTCAAATGAC CACACACTGT CATTTATTGC CCACCAGCCA 
ATGGATGCCA TCTGCGCAGT TGAGATCTCC AGTAAAGAGT ATCTGCTGTG TTTTAACAGC 
ATTGGGATAT ACACTGACTG CCAGGGCCGG AGATCGAGAC AACAGGAGTT GATGTGGCCG 
TCAAATCCCT CCTCTTGTTG TTACAACGCA CCATATCTCT CAGTGTATAG TGAGAATGCA 
GTTGATATCT TCGATGTGAA CTCCATGGAA TGGATTCAGA CTCTCCCACT CAAAAAGGTT 
CGACCCTTAA ACAGTGAAGG ATCATTAAAC CTTTTAGGGC TGGAGACAAT TAGATTAATA 
TATTTCAAAA ATAAGATGGC AGAAGGGGAT GAACTGGTGG TTCCTGAAAC ATCGGACAAT 
AGTCGGAAGC AGATGGTTAG AAACATCAAC AACAAGCGAC GCTTCTCCTT CAGGGTCCCA 
GAAGAGGAGA GGATGCAGCA GAGGAGAGAG ATGCTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ACCCAACTAA TTTTAACCAC ATAGCACACA TGGGCCCAGG GGATGGAATA 
CAGATCCTAA AAGACCTGCC TATGCCAGGC TTCCCTTATC CATCCCCAAA TCATCACTCC 
GGTCTGATCT CCTCTCCGAT CAACTTTGAG CACATCTATC ACATGACTGT TAATTCTGCT 
GAAAAATTTC TCTCTCCTGA TTCCATAAAC CCTGCATATT CCCCGTCTCT GCGCTCTGTC 
CCCGGTACAC CAAGCTTTAT GACTCTGAGG AACCCTCGGC CTCAGGAAAG CCGGACCGTG 
TTCAGCGGCT CAGTCAGTAT TCCATCCATC ACTAAATCCC GCCCTGAGCC CGGCCGCTCC 
ATGAGCGCCA GCAGCGGCCT TTCGGCAAGG TCATCTGCAC AGAATGGCAG TGCATTAAAG 
AGAGAATTCT CCGGAGGAAG CTATAATGCT AAGTGGCAGC CCATGCCCTC CCCATCAGAG 
GGCTCCTTGT CTTCCGGAGG CATGGACCAG GGAAGTGACG CCCCAGCCAG GGACTATGAC 
GGAGAGGACT CGGATTCTCC GAGGCACTCC ACAGCTTCCA ACAGCTCCAA CCTCAGCAGC 
CCGCCCAGCC CAGTGTCACC TCGGAAGACC AAGAGCCTGT CCCTGGAGAG CACCGACCGT 
GGGACCTGGG ACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_005622615.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622672.1 Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X3, mRNA.

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CloneID	OCe09379
Clone ID Related Accession (Same CDS sequence)	XM_005622615.1
Accession Version	XM_005622615.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase MRCK alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACACCGACCC CGTTGAGAGC ACATATGTAC GGAATCCGAA CGTCAAGGTT 
AACGTCCAGT CAAGGTCCAC ATCTCCTTCT ACATCTAGTG AAGCTGAGCC ACTTAAGATT 
GTAGACAGTA CTCCTCTTCC AGCTCACACA CCAACCTTAA GGAAAAAGGG ATGTCCTGGT 
TCAACTGGCT TTCCAGCTAA GCGCAAGACT CATCAGTTTT TTGTCAAATC TTTCACAACA 
CCTACCAGAT GTCACCAGTG CACCTCTCTG ATGGTTGGTT TGATAAGACA AGGCTGTTCC 
TGTGAGGTGT GCGGATTCTC ATGTCATATA ACTTGTGTAA ACAAAGCTCC AACTGCTTGT 
CCAGTCCCTC CTGAGCAGAC AAAAGGTCCT CTGGGGATAG ATCCACAGAA AGGAATAGGA 
ACAGCCTATG AAGGTCATGT CAGGATTCCG AAACCAGCTG GAGTAAAGAA AGGATGGCAG 
AGAGCCCTGG CTGTGGTTTG TGACTTTAAA CTCTTCCTGT ATGATGTCGC TGAAGGAAAA 
GCATCTCAGC CCAGCGTTGT GGTCAGTCAG GTGATTGACA TGAGGGATGA AGAATTTTCT 
GTGAGTTCAG TGTTGGCTTC TGATGTTATT CATGCAAGTC GAAAAGATAT ACCCTGTATA 
TTCAGAGTCA CAGCTTCCCA GCTCTCAGCA TCTGACAGCA GGTGTTCGGT CCTAATGCTC 
GCAGAGAGTG AGAGCGAGAG GAACAAGTGG GTGGGCGTGC TCAACGAGCT GCATAAGATT 
TTGAAGAAAA GCAAATTCAG AGACCGCTCC GTCTATGTTC CCAAGGAGGC TTATGACAGC 
ACTCTGCCCC TCATTAAAAC AACCCAGGCA GCTGCAATCA TAGATCACGA AAGGATAGCT 
CTGGGCAATG AAGAAGGATT ATTTGTTGTG CACATCACCA AAGATGAAAT TATTAGAGTT 
GGTGATAATA AGAAGATACA TCAGATTGAA CTCATTCCAA ATGATCAGCT TGTTGCTGTG 
ATCTCAGGAC GAAATCGTCA TGTACGACTT TTTCCTATAT CAGCTTTGGA CGGACGAGAG 
ACTGATTTTT ATAAGCTGGC AGAAACTAAA GGGTGTCAGA CCATAACTTC GGGCAAAGTG 
TGCCATGGAG CTCTCACATG CCTGTGTGTG GCTATGAAAA GGCAGGTCCT CTGTTATGAA 
CTATTTCAGA GCAAGACCCG TCACAGGAAA TTTAAGGAAA TTCAAGTCCC ATATAACGTG 
CAATGGATGG CGATCTTCAG TGAACAGCTC TGCGTGGGAT TCCAGTCGGG ATTCCTAAGA 
TACCCGTTGA ACGGAGAGGG AAGCCCATAC AGTATGCTCC ATTCAAATGA CCACACACTG 
TCATTTATTG CCCACCAGCC AATGGATGCC ATCTGCGCAG TTGAGATCTC CAGTAAAGAG 
TATCTGCTGT GTTTTAACAG CATTGGGATA TACACTGACT GCCAGGGCCG GAGATCGAGA 
CAACAGGAGT TGATGTGGCC GTCAAATCCC TCCTCTTGTT GTTACAACGC ACCATATCTC 
TCAGTGTATA GTGAGAATGC AGTTGATATC TTCGATGTGA ACTCCATGGA ATGGATTCAG 
ACTCTCCCAC TCAAAAAGGT TCGACCCTTA AACAGTGAAG GATCATTAAA CCTTTTAGGG 
CTGGAGACAA TTAGATTAAT ATATTTCAAA AATAAGATGG CAGAAGGGGA TGAACTGGTG 
GTTCCTGAAA CATCGGACAA TAGTCGGAAG CAGATGGTTA GAAACATCAA CAACAAGCGA 
CGCTTCTCCT TCAGGGTCCC AGAAGAGGAG AGGATGCAGC AGAGGAGAGA GATGCTACGA 
GATCCAGAAA TGAGAAATAA ATTAATTTCT AACCCAACTA ATTTTAACCA CATAGCACAC 
ATGGGCCCAG GGGATGGAAT ACAGATCCTA AAAGACCTGC CTATGCCAGG CTTCCCTTAT 
CCATCCCCAA ATCATCACTC CGGTCTGATC TCCTCTCCGA TCAACTTTGA GCACATCTAT 
CACATGACTG TTAATTCTGC TGAAAAATTT CTCTCTCCTG ATTCCATAAA CCCTGCATAT 
TCCCCGTCTC TGCGCTCTGT CCCCGGTACA CCAAGCTTTA TGACTCTGAG GAACCCTCGG 
CCTCAGGAAA GCCGGACCGT GTTCAGCGGC TCAGTCAGTA TTCCATCCAT CACTAAATCC 
CGCCCTGAGC CCGGCCGCTC CATGAGCGCC AGCAGCGGCC TTTCGGCAAG GTCATCTGCA 
CAGAATGGCA GTGCATTAAA GAGAGAATTC TCCGGAGGAA GCTATAATGC TAAGTGGCAG 
CCCATGCCCT CCCCATCAGA GGGCTCCTTG TCTTCCGGAG GCATGGACCA GGGAAGTGAC 
GCCCCAGCCA GGGACTATGA CGGAGAGGAC TCGGATTCTC CGAGGCACTC CACAGCTTCC 
AACAGCTCCA ACCTCAGCAG CCCGCCCAGC CCAGTGTCAC CTCGGAAGAC CAAGAGCCTG 
TCCCTGGAGA GCACCGACCG TGGGACCTGG GACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622672.1
CDS	384..5882
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPASLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKTLKEEIENLRKQIRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIRTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGLCSIEHQQEISKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLYENVSMRNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGARATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDTDPVESTYVRNPNVKVNVQSRSTSPSTSSEAEPLKIVDSTPLPAHTPTLRKKGCPGSTGFPAKRKTHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDVAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASDSRCSVLMLAESESERNKWVGVLNELHKILKKSKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHITKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPISALDGRETDFYKLAETKGCQTITSGKVCHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPSNPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRFSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPNHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPAYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNAKWQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGTWDP

Target ORF information:

RefSeq Version	XM_005622615.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622615.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACACCGACCC CGTTGAGAGC ACATATGTAC GGAATCCGAA CGTCAAGGTT 
AACGTCCAGT CAAGGTCCAC ATCTCCTTCT ACATCTAGTG AAGCTGAGCC ACTTAAGATT 
GTAGACAGTA CTCCTCTTCC AGCTCACACA CCAACCTTAA GGAAAAAGGG ATGTCCTGGT 
TCAACTGGCT TTCCAGCTAA GCGCAAGACT CATCAGTTTT TTGTCAAATC TTTCACAACA 
CCTACCAGAT GTCACCAGTG CACCTCTCTG ATGGTTGGTT TGATAAGACA AGGCTGTTCC 
TGTGAGGTGT GCGGATTCTC ATGTCATATA ACTTGTGTAA ACAAAGCTCC AACTGCTTGT 
CCAGTCCCTC CTGAGCAGAC AAAAGGTCCT CTGGGGATAG ATCCACAGAA AGGAATAGGA 
ACAGCCTATG AAGGTCATGT CAGGATTCCG AAACCAGCTG GAGTAAAGAA AGGATGGCAG 
AGAGCCCTGG CTGTGGTTTG TGACTTTAAA CTCTTCCTGT ATGATGTCGC TGAAGGAAAA 
GCATCTCAGC CCAGCGTTGT GGTCAGTCAG GTGATTGACA TGAGGGATGA AGAATTTTCT 
GTGAGTTCAG TGTTGGCTTC TGATGTTATT CATGCAAGTC GAAAAGATAT ACCCTGTATA 
TTCAGAGTCA CAGCTTCCCA GCTCTCAGCA TCTGACAGCA GGTGTTCGGT CCTAATGCTC 
GCAGAGAGTG AGAGCGAGAG GAACAAGTGG GTGGGCGTGC TCAACGAGCT GCATAAGATT 
TTGAAGAAAA GCAAATTCAG AGACCGCTCC GTCTATGTTC CCAAGGAGGC TTATGACAGC 
ACTCTGCCCC TCATTAAAAC AACCCAGGCA GCTGCAATCA TAGATCACGA AAGGATAGCT 
CTGGGCAATG AAGAAGGATT ATTTGTTGTG CACATCACCA AAGATGAAAT TATTAGAGTT 
GGTGATAATA AGAAGATACA TCAGATTGAA CTCATTCCAA ATGATCAGCT TGTTGCTGTG 
ATCTCAGGAC GAAATCGTCA TGTACGACTT TTTCCTATAT CAGCTTTGGA CGGACGAGAG 
ACTGATTTTT ATAAGCTGGC AGAAACTAAA GGGTGTCAGA CCATAACTTC GGGCAAAGTG 
TGCCATGGAG CTCTCACATG CCTGTGTGTG GCTATGAAAA GGCAGGTCCT CTGTTATGAA 
CTATTTCAGA GCAAGACCCG TCACAGGAAA TTTAAGGAAA TTCAAGTCCC ATATAACGTG 
CAATGGATGG CGATCTTCAG TGAACAGCTC TGCGTGGGAT TCCAGTCGGG ATTCCTAAGA 
TACCCGTTGA ACGGAGAGGG AAGCCCATAC AGTATGCTCC ATTCAAATGA CCACACACTG 
TCATTTATTG CCCACCAGCC AATGGATGCC ATCTGCGCAG TTGAGATCTC CAGTAAAGAG 
TATCTGCTGT GTTTTAACAG CATTGGGATA TACACTGACT GCCAGGGCCG GAGATCGAGA 
CAACAGGAGT TGATGTGGCC GTCAAATCCC TCCTCTTGTT GTTACAACGC ACCATATCTC 
TCAGTGTATA GTGAGAATGC AGTTGATATC TTCGATGTGA ACTCCATGGA ATGGATTCAG 
ACTCTCCCAC TCAAAAAGGT TCGACCCTTA AACAGTGAAG GATCATTAAA CCTTTTAGGG 
CTGGAGACAA TTAGATTAAT ATATTTCAAA AATAAGATGG CAGAAGGGGA TGAACTGGTG 
GTTCCTGAAA CATCGGACAA TAGTCGGAAG CAGATGGTTA GAAACATCAA CAACAAGCGA 
CGCTTCTCCT TCAGGGTCCC AGAAGAGGAG AGGATGCAGC AGAGGAGAGA GATGCTACGA 
GATCCAGAAA TGAGAAATAA ATTAATTTCT AACCCAACTA ATTTTAACCA CATAGCACAC 
ATGGGCCCAG GGGATGGAAT ACAGATCCTA AAAGACCTGC CTATGCCAGG CTTCCCTTAT 
CCATCCCCAA ATCATCACTC CGGTCTGATC TCCTCTCCGA TCAACTTTGA GCACATCTAT 
CACATGACTG TTAATTCTGC TGAAAAATTT CTCTCTCCTG ATTCCATAAA CCCTGCATAT 
TCCCCGTCTC TGCGCTCTGT CCCCGGTACA CCAAGCTTTA TGACTCTGAG GAACCCTCGG 
CCTCAGGAAA GCCGGACCGT GTTCAGCGGC TCAGTCAGTA TTCCATCCAT CACTAAATCC 
CGCCCTGAGC CCGGCCGCTC CATGAGCGCC AGCAGCGGCC TTTCGGCAAG GTCATCTGCA 
CAGAATGGCA GTGCATTAAA GAGAGAATTC TCCGGAGGAA GCTATAATGC TAAGTGGCAG 
CCCATGCCCT CCCCATCAGA GGGCTCCTTG TCTTCCGGAG GCATGGACCA GGGAAGTGAC 
GCCCCAGCCA GGGACTATGA CGGAGAGGAC TCGGATTCTC CGAGGCACTC CACAGCTTCC 
AACAGCTCCA ACCTCAGCAG CCCGCCCAGC CCAGTGTCAC CTCGGAAGAC CAAGAGCCTG 
TCCCTGGAGA GCACCGACCG TGGGACCTGG GACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_005622616.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622673.1 Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X4, mRNA.

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CloneID	OCe09380
Clone ID Related Accession (Same CDS sequence)	XM_005622616.1
Accession Version	XM_005622616.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase MRCK alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCGTCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACCGACC CCGTTGAGAG CACATATGTA CGGAATCCGA ACGTCAAGGT TAACGTCCAG 
TCAAGGTCCA CATCTCCTTC TACATCTAGT GAAGCTGAGC CACTTAAGAT TGTAGACAGT 
ACTCCTCTTC CAGCTCACAC ACCAACCTTA AGGAAAAAGG GATGTCCTGG TTCAACTGGC 
TTTCCAGCTA AGCGCAAGAC TCATCAGTTT TTTGTCAAAT CTTTCACAAC ACCTACCAGA 
TGTCACCAGT GCACCTCTCT GATGGTTGGT TTGATAAGAC AAGGCTGTTC CTGTGAGGTG 
TGCGGATTCT CATGTCATAT AACTTGTGTA AACAAAGCTC CAACTGCTTG TCCAGTCCCT 
CCTGAGCAGA CAAAAGGTCC TCTGGGGATA GATCCACAGA AAGGAATAGG AACAGCCTAT 
GAAGGTCATG TCAGGATTCC GAAACCAGCT GGAGTAAAGA AAGGATGGCA GAGAGCCCTG 
GCTGTGGTTT GTGACTTTAA ACTCTTCCTG TATGATGTCG CTGAAGGAAA AGCATCTCAG 
CCCAGCGTTG TGGTCAGTCA GGTGATTGAC ATGAGGGATG AAGAATTTTC TGTGAGTTCA 
GTGTTGGCTT CTGATGTTAT TCATGCAAGT CGAAAAGATA TACCCTGTAT ATTCAGAGTC 
ACAGCTTCCC AGCTCTCAGC ATCTGACAGC AGGTGTTCGG TCCTAATGCT CGCAGAGAGT 
GAGAGCGAGA GGAACAAGTG GGTGGGCGTG CTCAACGAGC TGCATAAGAT TTTGAAGAAA 
AGCAAATTCA GAGACCGCTC CGTCTATGTT CCCAAGGAGG CTTATGACAG CACTCTGCCC 
CTCATTAAAA CAACCCAGGC AGCTGCAATC ATAGATCACG AAAGGATAGC TCTGGGCAAT 
GAAGAAGGAT TATTTGTTGT GCACATCACC AAAGATGAAA TTATTAGAGT TGGTGATAAT 
AAGAAGATAC ATCAGATTGA ACTCATTCCA AATGATCAGC TTGTTGCTGT GATCTCAGGA 
CGAAATCGTC ATGTACGACT TTTTCCTATA TCAGCTTTGG ACGGACGAGA GACTGATTTT 
TATAAGCTGG CAGAAACTAA AGGGTGTCAG ACCATAACTT CGGGCAAAGT GTGCCATGGA 
GCTCTCACAT GCCTGTGTGT GGCTATGAAA AGGCAGGTCC TCTGTTATGA ACTATTTCAG 
AGCAAGACCC GTCACAGGAA ATTTAAGGAA ATTCAAGTCC CATATAACGT GCAATGGATG 
GCGATCTTCA GTGAACAGCT CTGCGTGGGA TTCCAGTCGG GATTCCTAAG ATACCCGTTG 
AACGGAGAGG GAAGCCCATA CAGTATGCTC CATTCAAATG ACCACACACT GTCATTTATT 
GCCCACCAGC CAATGGATGC CATCTGCGCA GTTGAGATCT CCAGTAAAGA GTATCTGCTG 
TGTTTTAACA GCATTGGGAT ATACACTGAC TGCCAGGGCC GGAGATCGAG ACAACAGGAG 
TTGATGTGGC CGTCAAATCC CTCCTCTTGT TGTTACAACG CACCATATCT CTCAGTGTAT 
AGTGAGAATG CAGTTGATAT CTTCGATGTG AACTCCATGG AATGGATTCA GACTCTCCCA 
CTCAAAAAGG TTCGACCCTT AAACAGTGAA GGATCATTAA ACCTTTTAGG GCTGGAGACA 
ATTAGATTAA TATATTTCAA AAATAAGATG GCAGAAGGGG ATGAACTGGT GGTTCCTGAA 
ACATCGGACA ATAGTCGGAA GCAGATGGTT AGAAACATCA ACAACAAGCG ACGCTTCTCC 
TTCAGGGTCC CAGAAGAGGA GAGGATGCAG CAGAGGAGAG AGATGCTACG AGATCCAGAA 
ATGAGAAATA AATTAATTTC TAACCCAACT AATTTTAACC ACATAGCACA CATGGGCCCA 
GGGGATGGAA TACAGATCCT AAAAGACCTG CCTATGCCAG GCTTCCCTTA TCCATCCCCA 
AATCATCACT CCGGTCTGAT CTCCTCTCCG ATCAACTTTG AGCACATCTA TCACATGACT 
GTTAATTCTG CTGAAAAATT TCTCTCTCCT GATTCCATAA ACCCTGCATA TTCCCCGTCT 
CTGCGCTCTG TCCCCGGTAC ACCAAGCTTT ATGACTCTGA GGAACCCTCG GCCTCAGGAA 
AGCCGGACCG TGTTCAGCGG CTCAGTCAGT ATTCCATCCA TCACTAAATC CCGCCCTGAG 
CCCGGCCGCT CCATGAGCGC CAGCAGCGGC CTTTCGGCAA GGTCATCTGC ACAGAATGGC 
AGTGCATTAA AGAGAGAATT CTCCGGAGGA AGCTATAATG CTAAGTGGCA GCCCATGCCC 
TCCCCATCAG AGGGCTCCTT GTCTTCCGGA GGCATGGACC AGGGAAGTGA CGCCCCAGCC 
AGGGACTATG ACGGAGAGGA CTCGGATTCT CCGAGGCACT CCACAGCTTC CAACAGCTCC 
AACCTCAGCA GCCCGCCCAG CCCAGTGTCA CCTCGGAAGA CCAAGAGCCT GTCCCTGGAG 
AGCACCGACC GTGGGACCTG GGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622673.1
CDS	384..5873
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPASLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKTLKEEIENLRKQIRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIRTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGLCSIEHQQEISKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLYENVSMRNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGARATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRTDPVESTYVRNPNVKVNVQSRSTSPSTSSEAEPLKIVDSTPLPAHTPTLRKKGCPGSTGFPAKRKTHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDVAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASDSRCSVLMLAESESERNKWVGVLNELHKILKKSKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHITKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPISALDGRETDFYKLAETKGCQTITSGKVCHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPSNPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRFSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPNHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPAYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNAKWQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGTWDP

Target ORF information:

RefSeq Version	XM_005622616.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622616.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCGTCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACCGACC CCGTTGAGAG CACATATGTA CGGAATCCGA ACGTCAAGGT TAACGTCCAG 
TCAAGGTCCA CATCTCCTTC TACATCTAGT GAAGCTGAGC CACTTAAGAT TGTAGACAGT 
ACTCCTCTTC CAGCTCACAC ACCAACCTTA AGGAAAAAGG GATGTCCTGG TTCAACTGGC 
TTTCCAGCTA AGCGCAAGAC TCATCAGTTT TTTGTCAAAT CTTTCACAAC ACCTACCAGA 
TGTCACCAGT GCACCTCTCT GATGGTTGGT TTGATAAGAC AAGGCTGTTC CTGTGAGGTG 
TGCGGATTCT CATGTCATAT AACTTGTGTA AACAAAGCTC CAACTGCTTG TCCAGTCCCT 
CCTGAGCAGA CAAAAGGTCC TCTGGGGATA GATCCACAGA AAGGAATAGG AACAGCCTAT 
GAAGGTCATG TCAGGATTCC GAAACCAGCT GGAGTAAAGA AAGGATGGCA GAGAGCCCTG 
GCTGTGGTTT GTGACTTTAA ACTCTTCCTG TATGATGTCG CTGAAGGAAA AGCATCTCAG 
CCCAGCGTTG TGGTCAGTCA GGTGATTGAC ATGAGGGATG AAGAATTTTC TGTGAGTTCA 
GTGTTGGCTT CTGATGTTAT TCATGCAAGT CGAAAAGATA TACCCTGTAT ATTCAGAGTC 
ACAGCTTCCC AGCTCTCAGC ATCTGACAGC AGGTGTTCGG TCCTAATGCT CGCAGAGAGT 
GAGAGCGAGA GGAACAAGTG GGTGGGCGTG CTCAACGAGC TGCATAAGAT TTTGAAGAAA 
AGCAAATTCA GAGACCGCTC CGTCTATGTT CCCAAGGAGG CTTATGACAG CACTCTGCCC 
CTCATTAAAA CAACCCAGGC AGCTGCAATC ATAGATCACG AAAGGATAGC TCTGGGCAAT 
GAAGAAGGAT TATTTGTTGT GCACATCACC AAAGATGAAA TTATTAGAGT TGGTGATAAT 
AAGAAGATAC ATCAGATTGA ACTCATTCCA AATGATCAGC TTGTTGCTGT GATCTCAGGA 
CGAAATCGTC ATGTACGACT TTTTCCTATA TCAGCTTTGG ACGGACGAGA GACTGATTTT 
TATAAGCTGG CAGAAACTAA AGGGTGTCAG ACCATAACTT CGGGCAAAGT GTGCCATGGA 
GCTCTCACAT GCCTGTGTGT GGCTATGAAA AGGCAGGTCC TCTGTTATGA ACTATTTCAG 
AGCAAGACCC GTCACAGGAA ATTTAAGGAA ATTCAAGTCC CATATAACGT GCAATGGATG 
GCGATCTTCA GTGAACAGCT CTGCGTGGGA TTCCAGTCGG GATTCCTAAG ATACCCGTTG 
AACGGAGAGG GAAGCCCATA CAGTATGCTC CATTCAAATG ACCACACACT GTCATTTATT 
GCCCACCAGC CAATGGATGC CATCTGCGCA GTTGAGATCT CCAGTAAAGA GTATCTGCTG 
TGTTTTAACA GCATTGGGAT ATACACTGAC TGCCAGGGCC GGAGATCGAG ACAACAGGAG 
TTGATGTGGC CGTCAAATCC CTCCTCTTGT TGTTACAACG CACCATATCT CTCAGTGTAT 
AGTGAGAATG CAGTTGATAT CTTCGATGTG AACTCCATGG AATGGATTCA GACTCTCCCA 
CTCAAAAAGG TTCGACCCTT AAACAGTGAA GGATCATTAA ACCTTTTAGG GCTGGAGACA 
ATTAGATTAA TATATTTCAA AAATAAGATG GCAGAAGGGG ATGAACTGGT GGTTCCTGAA 
ACATCGGACA ATAGTCGGAA GCAGATGGTT AGAAACATCA ACAACAAGCG ACGCTTCTCC 
TTCAGGGTCC CAGAAGAGGA GAGGATGCAG CAGAGGAGAG AGATGCTACG AGATCCAGAA 
ATGAGAAATA AATTAATTTC TAACCCAACT AATTTTAACC ACATAGCACA CATGGGCCCA 
GGGGATGGAA TACAGATCCT AAAAGACCTG CCTATGCCAG GCTTCCCTTA TCCATCCCCA 
AATCATCACT CCGGTCTGAT CTCCTCTCCG ATCAACTTTG AGCACATCTA TCACATGACT 
GTTAATTCTG CTGAAAAATT TCTCTCTCCT GATTCCATAA ACCCTGCATA TTCCCCGTCT 
CTGCGCTCTG TCCCCGGTAC ACCAAGCTTT ATGACTCTGA GGAACCCTCG GCCTCAGGAA 
AGCCGGACCG TGTTCAGCGG CTCAGTCAGT ATTCCATCCA TCACTAAATC CCGCCCTGAG 
CCCGGCCGCT CCATGAGCGC CAGCAGCGGC CTTTCGGCAA GGTCATCTGC ACAGAATGGC 
AGTGCATTAA AGAGAGAATT CTCCGGAGGA AGCTATAATG CTAAGTGGCA GCCCATGCCC 
TCCCCATCAG AGGGCTCCTT GTCTTCCGGA GGCATGGACC AGGGAAGTGA CGCCCCAGCC 
AGGGACTATG ACGGAGAGGA CTCGGATTCT CCGAGGCACT CCACAGCTTC CAACAGCTCC 
AACCTCAGCA GCCCGCCCAG CCCAGTGTCA CCTCGGAAGA CCAAGAGCCT GTCCCTGGAG 
AGCACCGACC GTGGGACCTG GGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_005622617.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622674.1 Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X5, mRNA.

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CloneID	OCe09381
Clone ID Related Accession (Same CDS sequence)	XM_005622617.1
Accession Version	XM_005622617.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase MRCK alpha isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACCGAC CCCGTTGAGA GCACATATGT ACGGAATCCG 
AACGTCAAGG TTAACGTCCA GTCAAGGTCC ACATCTCCTT CTACATCTAG TGAAGCTGAG 
CCACTTAAGA TTGTAGACAG TACTCCTCTT CCAGCTCACA CACCAACCTT AAGGAAAAAG 
GGATGTCCTG GTTCAACTGG CTTTCCAGCT AAGCGCAAGA CTCATCAGTT TTTTGTCAAA 
TCTTTCACAA CACCTACCAG ATGTCACCAG TGCACCTCTC TGATGGTTGG TTTGATAAGA 
CAAGGCTGTT CCTGTGAGGT GTGCGGATTC TCATGTCATA TAACTTGTGT AAACAAAGCT 
CCAACTGCTT GTCCAGTCCC TCCTGAGCAG ACAAAAGGTC CTCTGGGGAT AGATCCACAG 
AAAGGAATAG GAACAGCCTA TGAAGGTCAT GTCAGGATTC CGAAACCAGC TGGAGTAAAG 
AAAGGATGGC AGAGAGCCCT GGCTGTGGTT TGTGACTTTA AACTCTTCCT GTATGATGTC 
GCTGAAGGAA AAGCATCTCA GCCCAGCGTT GTGGTCAGTC AGGTGATTGA CATGAGGGAT 
GAAGAATTTT CTGTGAGTTC AGTGTTGGCT TCTGATGTTA TTCATGCAAG TCGAAAAGAT 
ATACCCTGTA TATTCAGAGT CACAGCTTCC CAGCTCTCAG CATCTGACAG CAGGTGTTCG 
GTCCTAATGC TCGCAGAGAG TGAGAGCGAG AGGAACAAGT GGGTGGGCGT GCTCAACGAG 
CTGCATAAGA TTTTGAAGAA AAGCAAATTC AGAGACCGCT CCGTCTATGT TCCCAAGGAG 
GCTTATGACA GCACTCTGCC CCTCATTAAA ACAACCCAGG CAGCTGCAAT CATAGATCAC 
GAAAGGATAG CTCTGGGCAA TGAAGAAGGA TTATTTGTTG TGCACATCAC CAAAGATGAA 
ATTATTAGAG TTGGTGATAA TAAGAAGATA CATCAGATTG AACTCATTCC AAATGATCAG 
CTTGTTGCTG TGATCTCAGG ACGAAATCGT CATGTACGAC TTTTTCCTAT ATCAGCTTTG 
GACGGACGAG AGACTGATTT TTATAAGCTG GCAGAAACTA AAGGGTGTCA GACCATAACT 
TCGGGCAAAG TGTGCCATGG AGCTCTCACA TGCCTGTGTG TGGCTATGAA AAGGCAGGTC 
CTCTGTTATG AACTATTTCA GAGCAAGACC CGTCACAGGA AATTTAAGGA AATTCAAGTC 
CCATATAACG TGCAATGGAT GGCGATCTTC AGTGAACAGC TCTGCGTGGG ATTCCAGTCG 
GGATTCCTAA GATACCCGTT GAACGGAGAG GGAAGCCCAT ACAGTATGCT CCATTCAAAT 
GACCACACAC TGTCATTTAT TGCCCACCAG CCAATGGATG CCATCTGCGC AGTTGAGATC 
TCCAGTAAAG AGTATCTGCT GTGTTTTAAC AGCATTGGGA TATACACTGA CTGCCAGGGC 
CGGAGATCGA GACAACAGGA GTTGATGTGG CCGTCAAATC CCTCCTCTTG TTGTTACAAC 
GCACCATATC TCTCAGTGTA TAGTGAGAAT GCAGTTGATA TCTTCGATGT GAACTCCATG 
GAATGGATTC AGACTCTCCC ACTCAAAAAG GTTCGACCCT TAAACAGTGA AGGATCATTA 
AACCTTTTAG GGCTGGAGAC AATTAGATTA ATATATTTCA AAAATAAGAT GGCAGAAGGG 
GATGAACTGG TGGTTCCTGA AACATCGGAC AATAGTCGGA AGCAGATGGT TAGAAACATC 
AACAACAAGC GACGCTTCTC CTTCAGGGTC CCAGAAGAGG AGAGGATGCA GCAGAGGAGA 
GAGATGCTAC GAGATCCAGA AATGAGAAAT AAATTAATTT CTAACCCAAC TAATTTTAAC 
CACATAGCAC ACATGGGCCC AGGGGATGGA ATACAGATCC TAAAAGACCT GCCTATGCCA 
GGCTTCCCTT ATCCATCCCC AAATCATCAC TCCGGTCTGA TCTCCTCTCC GATCAACTTT 
GAGCACATCT ATCACATGAC TGTTAATTCT GCTGAAAAAT TTCTCTCTCC TGATTCCATA 
AACCCTGCAT ATTCCCCGTC TCTGCGCTCT GTCCCCGGTA CACCAAGCTT TATGACTCTG 
AGGAACCCTC GGCCTCAGGA AAGCCGGACC GTGTTCAGCG GCTCAGTCAG TATTCCATCC 
ATCACTAAAT CCCGCCCTGA GCCCGGCCGC TCCATGAGCG CCAGCAGCGG CCTTTCGGCA 
AGGTCATCTG CACAGAATGG CAGTGCATTA AAGAGAGAAT TCTCCGGAGG AAGCTATAAT 
GCTAAGTGGC AGCCCATGCC CTCCCCATCA GAGGGCTCCT TGTCTTCCGG AGGCATGGAC 
CAGGGAAGTG ACGCCCCAGC CAGGGACTAT GACGGAGAGG ACTCGGATTC TCCGAGGCAC 
TCCACAGCTT CCAACAGCTC CAACCTCAGC AGCCCGCCCA GCCCAGTGTC ACCTCGGAAG 
ACCAAGAGCC TGTCCCTGGA GAGCACCGAC CGTGGGACCT GGGACCCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622674.1
CDS	384..5834
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPASLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKTLKEEIENLRKQIRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIRTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGLCSIEHQQEISKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLYENVSMRNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGARATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETDPVESTYVRNPNVKVNVQSRSTSPSTSSEAEPLKIVDSTPLPAHTPTLRKKGCPGSTGFPAKRKTHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDVAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASDSRCSVLMLAESESERNKWVGVLNELHKILKKSKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHITKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPISALDGRETDFYKLAETKGCQTITSGKVCHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPSNPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRFSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPNHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPAYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNAKWQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGTWDP

Target ORF information:

RefSeq Version	XM_005622617.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622617.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACCGAC CCCGTTGAGA GCACATATGT ACGGAATCCG 
AACGTCAAGG TTAACGTCCA GTCAAGGTCC ACATCTCCTT CTACATCTAG TGAAGCTGAG 
CCACTTAAGA TTGTAGACAG TACTCCTCTT CCAGCTCACA CACCAACCTT AAGGAAAAAG 
GGATGTCCTG GTTCAACTGG CTTTCCAGCT AAGCGCAAGA CTCATCAGTT TTTTGTCAAA 
TCTTTCACAA CACCTACCAG ATGTCACCAG TGCACCTCTC TGATGGTTGG TTTGATAAGA 
CAAGGCTGTT CCTGTGAGGT GTGCGGATTC TCATGTCATA TAACTTGTGT AAACAAAGCT 
CCAACTGCTT GTCCAGTCCC TCCTGAGCAG ACAAAAGGTC CTCTGGGGAT AGATCCACAG 
AAAGGAATAG GAACAGCCTA TGAAGGTCAT GTCAGGATTC CGAAACCAGC TGGAGTAAAG 
AAAGGATGGC AGAGAGCCCT GGCTGTGGTT TGTGACTTTA AACTCTTCCT GTATGATGTC 
GCTGAAGGAA AAGCATCTCA GCCCAGCGTT GTGGTCAGTC AGGTGATTGA CATGAGGGAT 
GAAGAATTTT CTGTGAGTTC AGTGTTGGCT TCTGATGTTA TTCATGCAAG TCGAAAAGAT 
ATACCCTGTA TATTCAGAGT CACAGCTTCC CAGCTCTCAG CATCTGACAG CAGGTGTTCG 
GTCCTAATGC TCGCAGAGAG TGAGAGCGAG AGGAACAAGT GGGTGGGCGT GCTCAACGAG 
CTGCATAAGA TTTTGAAGAA AAGCAAATTC AGAGACCGCT CCGTCTATGT TCCCAAGGAG 
GCTTATGACA GCACTCTGCC CCTCATTAAA ACAACCCAGG CAGCTGCAAT CATAGATCAC 
GAAAGGATAG CTCTGGGCAA TGAAGAAGGA TTATTTGTTG TGCACATCAC CAAAGATGAA 
ATTATTAGAG TTGGTGATAA TAAGAAGATA CATCAGATTG AACTCATTCC AAATGATCAG 
CTTGTTGCTG TGATCTCAGG ACGAAATCGT CATGTACGAC TTTTTCCTAT ATCAGCTTTG 
GACGGACGAG AGACTGATTT TTATAAGCTG GCAGAAACTA AAGGGTGTCA GACCATAACT 
TCGGGCAAAG TGTGCCATGG AGCTCTCACA TGCCTGTGTG TGGCTATGAA AAGGCAGGTC 
CTCTGTTATG AACTATTTCA GAGCAAGACC CGTCACAGGA AATTTAAGGA AATTCAAGTC 
CCATATAACG TGCAATGGAT GGCGATCTTC AGTGAACAGC TCTGCGTGGG ATTCCAGTCG 
GGATTCCTAA GATACCCGTT GAACGGAGAG GGAAGCCCAT ACAGTATGCT CCATTCAAAT 
GACCACACAC TGTCATTTAT TGCCCACCAG CCAATGGATG CCATCTGCGC AGTTGAGATC 
TCCAGTAAAG AGTATCTGCT GTGTTTTAAC AGCATTGGGA TATACACTGA CTGCCAGGGC 
CGGAGATCGA GACAACAGGA GTTGATGTGG CCGTCAAATC CCTCCTCTTG TTGTTACAAC 
GCACCATATC TCTCAGTGTA TAGTGAGAAT GCAGTTGATA TCTTCGATGT GAACTCCATG 
GAATGGATTC AGACTCTCCC ACTCAAAAAG GTTCGACCCT TAAACAGTGA AGGATCATTA 
AACCTTTTAG GGCTGGAGAC AATTAGATTA ATATATTTCA AAAATAAGAT GGCAGAAGGG 
GATGAACTGG TGGTTCCTGA AACATCGGAC AATAGTCGGA AGCAGATGGT TAGAAACATC 
AACAACAAGC GACGCTTCTC CTTCAGGGTC CCAGAAGAGG AGAGGATGCA GCAGAGGAGA 
GAGATGCTAC GAGATCCAGA AATGAGAAAT AAATTAATTT CTAACCCAAC TAATTTTAAC 
CACATAGCAC ACATGGGCCC AGGGGATGGA ATACAGATCC TAAAAGACCT GCCTATGCCA 
GGCTTCCCTT ATCCATCCCC AAATCATCAC TCCGGTCTGA TCTCCTCTCC GATCAACTTT 
GAGCACATCT ATCACATGAC TGTTAATTCT GCTGAAAAAT TTCTCTCTCC TGATTCCATA 
AACCCTGCAT ATTCCCCGTC TCTGCGCTCT GTCCCCGGTA CACCAAGCTT TATGACTCTG 
AGGAACCCTC GGCCTCAGGA AAGCCGGACC GTGTTCAGCG GCTCAGTCAG TATTCCATCC 
ATCACTAAAT CCCGCCCTGA GCCCGGCCGC TCCATGAGCG CCAGCAGCGG CCTTTCGGCA 
AGGTCATCTG CACAGAATGG CAGTGCATTA AAGAGAGAAT TCTCCGGAGG AAGCTATAAT 
GCTAAGTGGC AGCCCATGCC CTCCCCATCA GAGGGCTCCT TGTCTTCCGG AGGCATGGAC 
CAGGGAAGTG ACGCCCCAGC CAGGGACTAT GACGGAGAGG ACTCGGATTC TCCGAGGCAC 
TCCACAGCTT CCAACAGCTC CAACCTCAGC AGCCCGCCCA GCCCAGTGTC ACCTCGGAAG 
ACCAAGAGCC TGTCCCTGGA GAGCACCGAC CGTGGGACCT GGGACCCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_005622618.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622675.1 Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X6, mRNA.

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CloneID	OCe09382
Clone ID Related Accession (Same CDS sequence)	XM_005622618.1
Accession Version	XM_005622618.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase MRCK alpha isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC GTCCTGTACT CCAGCTAGCA AAGGCAGACG TATTGTAGAC 
AGTACTCCTC TTCCAGCTCA CACACCAACC TTAAGGAAAA AGGGATGTCC TGGTTCAACT 
GGCTTTCCAG CTAAGCGCAA GACTCATCAG TTTTTTGTCA AATCTTTCAC AACACCTACC 
AGATGTCACC AGTGCACCTC TCTGATGGTT GGTTTGATAA GACAAGGCTG TTCCTGTGAG 
GTGTGCGGAT TCTCATGTCA TATAACTTGT GTAAACAAAG CTCCAACTGC TTGTCCAGTC 
CCTCCTGAGC AGACAAAAGG TCCTCTGGGG ATAGATCCAC AGAAAGGAAT AGGAACAGCC 
TATGAAGGTC ATGTCAGGAT TCCGAAACCA GCTGGAGTAA AGAAAGGATG GCAGAGAGCC 
CTGGCTGTGG TTTGTGACTT TAAACTCTTC CTGTATGATG TCGCTGAAGG AAAAGCATCT 
CAGCCCAGCG TTGTGGTCAG TCAGGTGATT GACATGAGGG ATGAAGAATT TTCTGTGAGT 
TCAGTGTTGG CTTCTGATGT TATTCATGCA AGTCGAAAAG ATATACCCTG TATATTCAGA 
GTCACAGCTT CCCAGCTCTC AGCATCTGAC AGCAGGTGTT CGGTCCTAAT GCTCGCAGAG 
AGTGAGAGCG AGAGGAACAA GTGGGTGGGC GTGCTCAACG AGCTGCATAA GATTTTGAAG 
AAAAGCAAAT TCAGAGACCG CTCCGTCTAT GTTCCCAAGG AGGCTTATGA CAGCACTCTG 
CCCCTCATTA AAACAACCCA GGCAGCTGCA ATCATAGATC ACGAAAGGAT AGCTCTGGGC 
AATGAAGAAG GATTATTTGT TGTGCACATC ACCAAAGATG AAATTATTAG AGTTGGTGAT 
AATAAGAAGA TACATCAGAT TGAACTCATT CCAAATGATC AGCTTGTTGC TGTGATCTCA 
GGACGAAATC GTCATGTACG ACTTTTTCCT ATATCAGCTT TGGACGGACG AGAGACTGAT 
TTTTATAAGC TGGCAGAAAC TAAAGGGTGT CAGACCATAA CTTCGGGCAA AGTGTGCCAT 
GGAGCTCTCA CATGCCTGTG TGTGGCTATG AAAAGGCAGG TCCTCTGTTA TGAACTATTT 
CAGAGCAAGA CCCGTCACAG GAAATTTAAG GAAATTCAAG TCCCATATAA CGTGCAATGG 
ATGGCGATCT TCAGTGAACA GCTCTGCGTG GGATTCCAGT CGGGATTCCT AAGATACCCG 
TTGAACGGAG AGGGAAGCCC ATACAGTATG CTCCATTCAA ATGACCACAC ACTGTCATTT 
ATTGCCCACC AGCCAATGGA TGCCATCTGC GCAGTTGAGA TCTCCAGTAA AGAGTATCTG 
CTGTGTTTTA ACAGCATTGG GATATACACT GACTGCCAGG GCCGGAGATC GAGACAACAG 
GAGTTGATGT GGCCGTCAAA TCCCTCCTCT TGTTGTTACA ACGCACCATA TCTCTCAGTG 
TATAGTGAGA ATGCAGTTGA TATCTTCGAT GTGAACTCCA TGGAATGGAT TCAGACTCTC 
CCACTCAAAA AGGTTCGACC CTTAAACAGT GAAGGATCAT TAAACCTTTT AGGGCTGGAG 
ACAATTAGAT TAATATATTT CAAAAATAAG ATGGCAGAAG GGGATGAACT GGTGGTTCCT 
GAAACATCGG ACAATAGTCG GAAGCAGATG GTTAGAAACA TCAACAACAA GCGACGCTTC 
TCCTTCAGGG TCCCAGAAGA GGAGAGGATG CAGCAGAGGA GAGAGATGCT ACGAGATCCA 
GAAATGAGAA ATAAATTAAT TTCTAACCCA ACTAATTTTA ACCACATAGC ACACATGGGC 
CCAGGGGATG GAATACAGAT CCTAAAAGAC CTGCCTATGC CAGGCTTCCC TTATCCATCC 
CCAAATCATC ACTCCGGTCT GATCTCCTCT CCGATCAACT TTGAGCACAT CTATCACATG 
ACTGTTAATT CTGCTGAAAA ATTTCTCTCT CCTGATTCCA TAAACCCTGC ATATTCCCCG 
TCTCTGCGCT CTGTCCCCGG TACACCAAGC TTTATGACTC TGAGGAACCC TCGGCCTCAG 
GAAAGCCGGA CCGTGTTCAG CGGCTCAGTC AGTATTCCAT CCATCACTAA ATCCCGCCCT 
GAGCCCGGCC GCTCCATGAG CGCCAGCAGC GGCCTTTCGG CAAGGTCATC TGCACAGAAT 
GGCAGTGCAT TAAAGAGAGA ATTCTCCGGA GGAAGCTATA ATGCTAAGTG GCAGCCCATG 
CCCTCCCCAT CAGAGGGCTC CTTGTCTTCC GGAGGCATGG ACCAGGGAAG TGACGCCCCA 
GCCAGGGACT ATGACGGAGA GGACTCGGAT TCTCCGAGGC ACTCCACAGC TTCCAACAGC 
TCCAACCTCA GCAGCCCGCC CAGCCCAGTG TCACCTCGGA AGACCAAGAG CCTGTCCCTG 
GAGAGCACCG ACCGTGGGAC CTGGGACCCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622675.1
CDS	384..5816
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPASLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKTLKEEIENLRKQIRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIRTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGLCSIEHQQEISKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLYENVSMRNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGARATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRIVDSTPLPAHTPTLRKKGCPGSTGFPAKRKTHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDVAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASDSRCSVLMLAESESERNKWVGVLNELHKILKKSKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHITKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPISALDGRETDFYKLAETKGCQTITSGKVCHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPSNPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRFSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPNHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPAYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNAKWQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGTWDP

Target ORF information:

RefSeq Version	XM_005622618.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622618.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC GTCCTGTACT CCAGCTAGCA AAGGCAGACG TATTGTAGAC 
AGTACTCCTC TTCCAGCTCA CACACCAACC TTAAGGAAAA AGGGATGTCC TGGTTCAACT 
GGCTTTCCAG CTAAGCGCAA GACTCATCAG TTTTTTGTCA AATCTTTCAC AACACCTACC 
AGATGTCACC AGTGCACCTC TCTGATGGTT GGTTTGATAA GACAAGGCTG TTCCTGTGAG 
GTGTGCGGAT TCTCATGTCA TATAACTTGT GTAAACAAAG CTCCAACTGC TTGTCCAGTC 
CCTCCTGAGC AGACAAAAGG TCCTCTGGGG ATAGATCCAC AGAAAGGAAT AGGAACAGCC 
TATGAAGGTC ATGTCAGGAT TCCGAAACCA GCTGGAGTAA AGAAAGGATG GCAGAGAGCC 
CTGGCTGTGG TTTGTGACTT TAAACTCTTC CTGTATGATG TCGCTGAAGG AAAAGCATCT 
CAGCCCAGCG TTGTGGTCAG TCAGGTGATT GACATGAGGG ATGAAGAATT TTCTGTGAGT 
TCAGTGTTGG CTTCTGATGT TATTCATGCA AGTCGAAAAG ATATACCCTG TATATTCAGA 
GTCACAGCTT CCCAGCTCTC AGCATCTGAC AGCAGGTGTT CGGTCCTAAT GCTCGCAGAG 
AGTGAGAGCG AGAGGAACAA GTGGGTGGGC GTGCTCAACG AGCTGCATAA GATTTTGAAG 
AAAAGCAAAT TCAGAGACCG CTCCGTCTAT GTTCCCAAGG AGGCTTATGA CAGCACTCTG 
CCCCTCATTA AAACAACCCA GGCAGCTGCA ATCATAGATC ACGAAAGGAT AGCTCTGGGC 
AATGAAGAAG GATTATTTGT TGTGCACATC ACCAAAGATG AAATTATTAG AGTTGGTGAT 
AATAAGAAGA TACATCAGAT TGAACTCATT CCAAATGATC AGCTTGTTGC TGTGATCTCA 
GGACGAAATC GTCATGTACG ACTTTTTCCT ATATCAGCTT TGGACGGACG AGAGACTGAT 
TTTTATAAGC TGGCAGAAAC TAAAGGGTGT CAGACCATAA CTTCGGGCAA AGTGTGCCAT 
GGAGCTCTCA CATGCCTGTG TGTGGCTATG AAAAGGCAGG TCCTCTGTTA TGAACTATTT 
CAGAGCAAGA CCCGTCACAG GAAATTTAAG GAAATTCAAG TCCCATATAA CGTGCAATGG 
ATGGCGATCT TCAGTGAACA GCTCTGCGTG GGATTCCAGT CGGGATTCCT AAGATACCCG 
TTGAACGGAG AGGGAAGCCC ATACAGTATG CTCCATTCAA ATGACCACAC ACTGTCATTT 
ATTGCCCACC AGCCAATGGA TGCCATCTGC GCAGTTGAGA TCTCCAGTAA AGAGTATCTG 
CTGTGTTTTA ACAGCATTGG GATATACACT GACTGCCAGG GCCGGAGATC GAGACAACAG 
GAGTTGATGT GGCCGTCAAA TCCCTCCTCT TGTTGTTACA ACGCACCATA TCTCTCAGTG 
TATAGTGAGA ATGCAGTTGA TATCTTCGAT GTGAACTCCA TGGAATGGAT TCAGACTCTC 
CCACTCAAAA AGGTTCGACC CTTAAACAGT GAAGGATCAT TAAACCTTTT AGGGCTGGAG 
ACAATTAGAT TAATATATTT CAAAAATAAG ATGGCAGAAG GGGATGAACT GGTGGTTCCT 
GAAACATCGG ACAATAGTCG GAAGCAGATG GTTAGAAACA TCAACAACAA GCGACGCTTC 
TCCTTCAGGG TCCCAGAAGA GGAGAGGATG CAGCAGAGGA GAGAGATGCT ACGAGATCCA 
GAAATGAGAA ATAAATTAAT TTCTAACCCA ACTAATTTTA ACCACATAGC ACACATGGGC 
CCAGGGGATG GAATACAGAT CCTAAAAGAC CTGCCTATGC CAGGCTTCCC TTATCCATCC 
CCAAATCATC ACTCCGGTCT GATCTCCTCT CCGATCAACT TTGAGCACAT CTATCACATG 
ACTGTTAATT CTGCTGAAAA ATTTCTCTCT CCTGATTCCA TAAACCCTGC ATATTCCCCG 
TCTCTGCGCT CTGTCCCCGG TACACCAAGC TTTATGACTC TGAGGAACCC TCGGCCTCAG 
GAAAGCCGGA CCGTGTTCAG CGGCTCAGTC AGTATTCCAT CCATCACTAA ATCCCGCCCT 
GAGCCCGGCC GCTCCATGAG CGCCAGCAGC GGCCTTTCGG CAAGGTCATC TGCACAGAAT 
GGCAGTGCAT TAAAGAGAGA ATTCTCCGGA GGAAGCTATA ATGCTAAGTG GCAGCCCATG 
CCCTCCCCAT CAGAGGGCTC CTTGTCTTCC GGAGGCATGG ACCAGGGAAG TGACGCCCCA 
GCCAGGGACT ATGACGGAGA GGACTCGGAT TCTCCGAGGC ACTCCACAGC TTCCAACAGC 
TCCAACCTCA GCAGCCCGCC CAGCCCAGTG TCACCTCGGA AGACCAAGAG CCTGTCCCTG 
GAGAGCACCG ACCGTGGGAC CTGGGACCCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_005622619.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622676.1 Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X7, mRNA.

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CloneID	OCe09383
Clone ID Related Accession (Same CDS sequence)	XM_005622619.1
Accession Version	XM_005622619.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase MRCK alpha isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACATTGTAGA CAGTACTCCT CTTCCAGCTC ACACACCAAC CTTAAGGAAA 
AAGGGATGTC CTGGTTCAAC TGGCTTTCCA GCTAAGCGCA AGACTCATCA GTTTTTTGTC 
AAATCTTTCA CAACACCTAC CAGATGTCAC CAGTGCACCT CTCTGATGGT TGGTTTGATA 
AGACAAGGCT GTTCCTGTGA GGTGTGCGGA TTCTCATGTC ATATAACTTG TGTAAACAAA 
GCTCCAACTG CTTGTCCAGT CCCTCCTGAG CAGACAAAAG GTCCTCTGGG GATAGATCCA 
CAGAAAGGAA TAGGAACAGC CTATGAAGGT CATGTCAGGA TTCCGAAACC AGCTGGAGTA 
AAGAAAGGAT GGCAGAGAGC CCTGGCTGTG GTTTGTGACT TTAAACTCTT CCTGTATGAT 
GTCGCTGAAG GAAAAGCATC TCAGCCCAGC GTTGTGGTCA GTCAGGTGAT TGACATGAGG 
GATGAAGAAT TTTCTGTGAG TTCAGTGTTG GCTTCTGATG TTATTCATGC AAGTCGAAAA 
GATATACCCT GTATATTCAG AGTCACAGCT TCCCAGCTCT CAGCATCTGA CAGCAGGTGT 
TCGGTCCTAA TGCTCGCAGA GAGTGAGAGC GAGAGGAACA AGTGGGTGGG CGTGCTCAAC 
GAGCTGCATA AGATTTTGAA GAAAAGCAAA TTCAGAGACC GCTCCGTCTA TGTTCCCAAG 
GAGGCTTATG ACAGCACTCT GCCCCTCATT AAAACAACCC AGGCAGCTGC AATCATAGAT 
CACGAAAGGA TAGCTCTGGG CAATGAAGAA GGATTATTTG TTGTGCACAT CACCAAAGAT 
GAAATTATTA GAGTTGGTGA TAATAAGAAG ATACATCAGA TTGAACTCAT TCCAAATGAT 
CAGCTTGTTG CTGTGATCTC AGGACGAAAT CGTCATGTAC GACTTTTTCC TATATCAGCT 
TTGGACGGAC GAGAGACTGA TTTTTATAAG CTGGCAGAAA CTAAAGGGTG TCAGACCATA 
ACTTCGGGCA AAGTGTGCCA TGGAGCTCTC ACATGCCTGT GTGTGGCTAT GAAAAGGCAG 
GTCCTCTGTT ATGAACTATT TCAGAGCAAG ACCCGTCACA GGAAATTTAA GGAAATTCAA 
GTCCCATATA ACGTGCAATG GATGGCGATC TTCAGTGAAC AGCTCTGCGT GGGATTCCAG 
TCGGGATTCC TAAGATACCC GTTGAACGGA GAGGGAAGCC CATACAGTAT GCTCCATTCA 
AATGACCACA CACTGTCATT TATTGCCCAC CAGCCAATGG ATGCCATCTG CGCAGTTGAG 
ATCTCCAGTA AAGAGTATCT GCTGTGTTTT AACAGCATTG GGATATACAC TGACTGCCAG 
GGCCGGAGAT CGAGACAACA GGAGTTGATG TGGCCGTCAA ATCCCTCCTC TTGTTGTTAC 
AACGCACCAT ATCTCTCAGT GTATAGTGAG AATGCAGTTG ATATCTTCGA TGTGAACTCC 
ATGGAATGGA TTCAGACTCT CCCACTCAAA AAGGTTCGAC CCTTAAACAG TGAAGGATCA 
TTAAACCTTT TAGGGCTGGA GACAATTAGA TTAATATATT TCAAAAATAA GATGGCAGAA 
GGGGATGAAC TGGTGGTTCC TGAAACATCG GACAATAGTC GGAAGCAGAT GGTTAGAAAC 
ATCAACAACA AGCGACGCTT CTCCTTCAGG GTCCCAGAAG AGGAGAGGAT GCAGCAGAGG 
AGAGAGATGC TACGAGATCC AGAAATGAGA AATAAATTAA TTTCTAACCC AACTAATTTT 
AACCACATAG CACACATGGG CCCAGGGGAT GGAATACAGA TCCTAAAAGA CCTGCCTATG 
CCAGGCTTCC CTTATCCATC CCCAAATCAT CACTCCGGTC TGATCTCCTC TCCGATCAAC 
TTTGAGCACA TCTATCACAT GACTGTTAAT TCTGCTGAAA AATTTCTCTC TCCTGATTCC 
ATAAACCCTG CATATTCCCC GTCTCTGCGC TCTGTCCCCG GTACACCAAG CTTTATGACT 
CTGAGGAACC CTCGGCCTCA GGAAAGCCGG ACCGTGTTCA GCGGCTCAGT CAGTATTCCA 
TCCATCACTA AATCCCGCCC TGAGCCCGGC CGCTCCATGA GCGCCAGCAG CGGCCTTTCG 
GCAAGGTCAT CTGCACAGAA TGGCAGTGCA TTAAAGAGAG AATTCTCCGG AGGAAGCTAT 
AATGCTAAGT GGCAGCCCAT GCCCTCCCCA TCAGAGGGCT CCTTGTCTTC CGGAGGCATG 
GACCAGGGAA GTGACGCCCC AGCCAGGGAC TATGACGGAG AGGACTCGGA TTCTCCGAGG 
CACTCCACAG CTTCCAACAG CTCCAACCTC AGCAGCCCGC CCAGCCCAGT GTCACCTCGG 
AAGACCAAGA GCCTGTCCCT GGAGAGCACC GACCGTGGGA CCTGGGACCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622676.1
CDS	384..5777
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPASLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKTLKEEIENLRKQIRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIRTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGLCSIEHQQEISKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLYENVSMRNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGARATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDIVDSTPLPAHTPTLRKKGCPGSTGFPAKRKTHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDVAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASDSRCSVLMLAESESERNKWVGVLNELHKILKKSKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHITKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPISALDGRETDFYKLAETKGCQTITSGKVCHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPSNPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRFSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPNHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPAYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNAKWQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGTWDP

Target ORF information:

RefSeq Version	XM_005622619.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622619.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACATTGTAGA CAGTACTCCT CTTCCAGCTC ACACACCAAC CTTAAGGAAA 
AAGGGATGTC CTGGTTCAAC TGGCTTTCCA GCTAAGCGCA AGACTCATCA GTTTTTTGTC 
AAATCTTTCA CAACACCTAC CAGATGTCAC CAGTGCACCT CTCTGATGGT TGGTTTGATA 
AGACAAGGCT GTTCCTGTGA GGTGTGCGGA TTCTCATGTC ATATAACTTG TGTAAACAAA 
GCTCCAACTG CTTGTCCAGT CCCTCCTGAG CAGACAAAAG GTCCTCTGGG GATAGATCCA 
CAGAAAGGAA TAGGAACAGC CTATGAAGGT CATGTCAGGA TTCCGAAACC AGCTGGAGTA 
AAGAAAGGAT GGCAGAGAGC CCTGGCTGTG GTTTGTGACT TTAAACTCTT CCTGTATGAT 
GTCGCTGAAG GAAAAGCATC TCAGCCCAGC GTTGTGGTCA GTCAGGTGAT TGACATGAGG 
GATGAAGAAT TTTCTGTGAG TTCAGTGTTG GCTTCTGATG TTATTCATGC AAGTCGAAAA 
GATATACCCT GTATATTCAG AGTCACAGCT TCCCAGCTCT CAGCATCTGA CAGCAGGTGT 
TCGGTCCTAA TGCTCGCAGA GAGTGAGAGC GAGAGGAACA AGTGGGTGGG CGTGCTCAAC 
GAGCTGCATA AGATTTTGAA GAAAAGCAAA TTCAGAGACC GCTCCGTCTA TGTTCCCAAG 
GAGGCTTATG ACAGCACTCT GCCCCTCATT AAAACAACCC AGGCAGCTGC AATCATAGAT 
CACGAAAGGA TAGCTCTGGG CAATGAAGAA GGATTATTTG TTGTGCACAT CACCAAAGAT 
GAAATTATTA GAGTTGGTGA TAATAAGAAG ATACATCAGA TTGAACTCAT TCCAAATGAT 
CAGCTTGTTG CTGTGATCTC AGGACGAAAT CGTCATGTAC GACTTTTTCC TATATCAGCT 
TTGGACGGAC GAGAGACTGA TTTTTATAAG CTGGCAGAAA CTAAAGGGTG TCAGACCATA 
ACTTCGGGCA AAGTGTGCCA TGGAGCTCTC ACATGCCTGT GTGTGGCTAT GAAAAGGCAG 
GTCCTCTGTT ATGAACTATT TCAGAGCAAG ACCCGTCACA GGAAATTTAA GGAAATTCAA 
GTCCCATATA ACGTGCAATG GATGGCGATC TTCAGTGAAC AGCTCTGCGT GGGATTCCAG 
TCGGGATTCC TAAGATACCC GTTGAACGGA GAGGGAAGCC CATACAGTAT GCTCCATTCA 
AATGACCACA CACTGTCATT TATTGCCCAC CAGCCAATGG ATGCCATCTG CGCAGTTGAG 
ATCTCCAGTA AAGAGTATCT GCTGTGTTTT AACAGCATTG GGATATACAC TGACTGCCAG 
GGCCGGAGAT CGAGACAACA GGAGTTGATG TGGCCGTCAA ATCCCTCCTC TTGTTGTTAC 
AACGCACCAT ATCTCTCAGT GTATAGTGAG AATGCAGTTG ATATCTTCGA TGTGAACTCC 
ATGGAATGGA TTCAGACTCT CCCACTCAAA AAGGTTCGAC CCTTAAACAG TGAAGGATCA 
TTAAACCTTT TAGGGCTGGA GACAATTAGA TTAATATATT TCAAAAATAA GATGGCAGAA 
GGGGATGAAC TGGTGGTTCC TGAAACATCG GACAATAGTC GGAAGCAGAT GGTTAGAAAC 
ATCAACAACA AGCGACGCTT CTCCTTCAGG GTCCCAGAAG AGGAGAGGAT GCAGCAGAGG 
AGAGAGATGC TACGAGATCC AGAAATGAGA AATAAATTAA TTTCTAACCC AACTAATTTT 
AACCACATAG CACACATGGG CCCAGGGGAT GGAATACAGA TCCTAAAAGA CCTGCCTATG 
CCAGGCTTCC CTTATCCATC CCCAAATCAT CACTCCGGTC TGATCTCCTC TCCGATCAAC 
TTTGAGCACA TCTATCACAT GACTGTTAAT TCTGCTGAAA AATTTCTCTC TCCTGATTCC 
ATAAACCCTG CATATTCCCC GTCTCTGCGC TCTGTCCCCG GTACACCAAG CTTTATGACT 
CTGAGGAACC CTCGGCCTCA GGAAAGCCGG ACCGTGTTCA GCGGCTCAGT CAGTATTCCA 
TCCATCACTA AATCCCGCCC TGAGCCCGGC CGCTCCATGA GCGCCAGCAG CGGCCTTTCG 
GCAAGGTCAT CTGCACAGAA TGGCAGTGCA TTAAAGAGAG AATTCTCCGG AGGAAGCTAT 
AATGCTAAGT GGCAGCCCAT GCCCTCCCCA TCAGAGGGCT CCTTGTCTTC CGGAGGCATG 
GACCAGGGAA GTGACGCCCC AGCCAGGGAC TATGACGGAG AGGACTCGGA TTCTCCGAGG 
CACTCCACAG CTTCCAACAG CTCCAACCTC AGCAGCCCGC CCAGCCCAGT GTCACCTCGG 
AAGACCAAGA GCCTGTCCCT GGAGAGCACC GACCGTGGGA CCTGGGACCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_005622620.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622677.1 Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X8, mRNA.

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CloneID	OCe09384
Clone ID Related Accession (Same CDS sequence)	XM_005622620.1
Accession Version	XM_005622620.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase MRCK alpha isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGCTA GCAAAGGCAG ACGTACCGAC CCCGTTGAGA GCACATATGT ACGGAATCCG 
AACGTCAAGG TTAACGTCCA GTCAAGGTCC ACATCTCCTT CTACATCTAG TGAAGCTGAG 
CCACTTAAGA TTGTAGACAG TACTCCTCTT CCAGCTCACA CACCAACCTT AAGGAAAAAG 
GGATGTCCTG GTTCAACTGG CTTTCCAGCT AAGCGCAAGA CTCATCAGTT TTTTGTCAAA 
TCTTTCACAA CACCTACCAG ATGTCACCAG TGCACCTCTC TGATGGTTGG TTTGATAAGA 
CAAGGCTGTT CCTGTGAGGT GTGCGGATTC TCATGTCATA TAACTTGTGT AAACAAAGCT 
CCAACTGCTT GTCCAGTCCC TCCTGAGCAG ACAAAAGGTC CTCTGGGGAT AGATCCACAG 
AAAGGAATAG GAACAGCCTA TGAAGGTCAT GTCAGGATTC CGAAACCAGC TGGAGTAAAG 
AAAGGATGGC AGAGAGCCCT GGCTGTGGTT TGTGACTTTA AACTCTTCCT GTATGATGTC 
GCTGAAGGAA AAGCATCTCA GCCCAGCGTT GTGGTCAGTC AGGTGATTGA CATGAGGGAT 
GAAGAATTTT CTGTGAGTTC AGTGTTGGCT TCTGATGTTA TTCATGCAAG TCGAAAAGAT 
ATACCCTGTA TATTCAGAGT CACAGCTTCC CAGCTCTCAG CATCTGACAG CAGGTGTTCG 
GTCCTAATGC TCGCAGAGAG TGAGAGCGAG AGGAACAAGT GGGTGGGCGT GCTCAACGAG 
CTGCATAAGA TTTTGAAGAA AAGCAAATTC AGAGACCGCT CCGTCTATGT TCCCAAGGAG 
GCTTATGACA GCACTCTGCC CCTCATTAAA ACAACCCAGG CAGCTGCAAT CATAGATCAC 
GAAAGGATAG CTCTGGGCAA TGAAGAAGGA TTATTTGTTG TGCACATCAC CAAAGATGAA 
ATTATTAGAG TTGGTGATAA TAAGAAGATA CATCAGATTG AACTCATTCC AAATGATCAG 
CTTGTTGCTG TGATCTCAGG ACGAAATCGT CATGTACGAC TTTTTCCTAT ATCAGCTTTG 
GACGGACGAG AGACTGATTT TTATAAGCTG GCAGAAACTA AAGGGTGTCA GACCATAACT 
TCGGGCAAAG TGTGCCATGG AGCTCTCACA TGCCTGTGTG TGGCTATGAA AAGGCAGGTC 
CTCTGTTATG AACTATTTCA GAGCAAGACC CGTCACAGGA AATTTAAGGA AATTCAAGTC 
CCATATAACG TGCAATGGAT GGCGATCTTC AGTGAACAGC TCTGCGTGGG ATTCCAGTCG 
GGATTCCTAA GATACCCGTT GAACGGAGAG GGAAGCCCAT ACAGTATGCT CCATTCAAAT 
GACCACACAC TGTCATTTAT TGCCCACCAG CCAATGGATG CCATCTGCGC AGTTGAGATC 
TCCAGTAAAG AGTATCTGCT GTGTTTTAAC AGCATTGGGA TATACACTGA CTGCCAGGGC 
CGGAGATCGA GACAACAGGA GTTGATGTGG CCGTCAAATC CCTCCTCTTG TTGTTACAAC 
GCACCATATC TCTCAGTGTA TAGTGAGAAT GCAGTTGATA TCTTCGATGT GAACTCCATG 
GAATGGATTC AGACTCTCCC ACTCAAAAAG GTTCGACCCT TAAACAGTGA AGGATCATTA 
AACCTTTTAG GGCTGGAGAC AATTAGATTA ATATATTTCA AAAATAAGAT GGCAGAAGGG 
GATGAACTGG TGGTTCCTGA AACATCGGAC AATAGTCGGA AGCAGATGGT TAGAAACATC 
AACAACAAGC GACGCTTCTC CTTCAGGGTC CCAGAAGAGG AGAGGATGCA GCAGAGGAGA 
GAGATGCTAC GAGATCCAGA AATGAGAAAT AAATTAATTT CTAACCCAAC TAATTTTAAC 
CACATAGCAC ACATGGGCCC AGGGGATGGA ATACAGATCC TAAAAGACCT GCCTATGCCA 
GGCTTCCCTT ATCCATCCCC AAATCATCAC TCCGGTCTGA TCTCCTCTCC GATCAACTTT 
GAGCACATCT ATCACATGAC TGTTAATTCT GCTGAAAAAT TTCTCTCTCC TGATTCCATA 
AACCCTGCAT ATTCCCCGTC TCTGCGCTCT GTCCCCGGTA CACCAAGCTT TATGACTCTG 
AGGAACCCTC GGCCTCAGGA AAGCCGGACC GTGTTCAGCG GCTCAGTCAG TATTCCATCC 
ATCACTAAAT CCCGCCCTGA GCCCGGCCGC TCCATGAGCG CCAGCAGCGG CCTTTCGGCA 
AGGTCATCTG CACAGAATGG CAGTGCATTA AAGAGAGAAT TCTCCGGAGG AAGCTATAAT 
GCTAAGTGGC AGCCCATGCC CTCCCCATCA GAGGGCTCCT TGTCTTCCGG AGGCATGGAC 
CAGGGAAGTG ACGCCCCAGC CAGGGACTAT GACGGAGAGG ACTCGGATTC TCCGAGGCAC 
TCCACAGCTT CCAACAGCTC CAACCTCAGC AGCCCGCCCA GCCCAGTGTC ACCTCGGAAG 
ACCAAGAGCC TGTCCCTGGA GAGCACCGAC CGTGGGACCT GGGACCCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622677.1
CDS	384..5774
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPASLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKTLKEEIENLRKQIRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIRTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGLCSIEHQQEISKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLYENVSMRNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGARATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKASKGRRTDPVESTYVRNPNVKVNVQSRSTSPSTSSEAEPLKIVDSTPLPAHTPTLRKKGCPGSTGFPAKRKTHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDVAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASDSRCSVLMLAESESERNKWVGVLNELHKILKKSKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHITKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPISALDGRETDFYKLAETKGCQTITSGKVCHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPSNPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRFSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPNHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPAYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNAKWQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGTWDP

Target ORF information:

RefSeq Version	XM_005622620.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622620.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGCTA GCAAAGGCAG ACGTACCGAC CCCGTTGAGA GCACATATGT ACGGAATCCG 
AACGTCAAGG TTAACGTCCA GTCAAGGTCC ACATCTCCTT CTACATCTAG TGAAGCTGAG 
CCACTTAAGA TTGTAGACAG TACTCCTCTT CCAGCTCACA CACCAACCTT AAGGAAAAAG 
GGATGTCCTG GTTCAACTGG CTTTCCAGCT AAGCGCAAGA CTCATCAGTT TTTTGTCAAA 
TCTTTCACAA CACCTACCAG ATGTCACCAG TGCACCTCTC TGATGGTTGG TTTGATAAGA 
CAAGGCTGTT CCTGTGAGGT GTGCGGATTC TCATGTCATA TAACTTGTGT AAACAAAGCT 
CCAACTGCTT GTCCAGTCCC TCCTGAGCAG ACAAAAGGTC CTCTGGGGAT AGATCCACAG 
AAAGGAATAG GAACAGCCTA TGAAGGTCAT GTCAGGATTC CGAAACCAGC TGGAGTAAAG 
AAAGGATGGC AGAGAGCCCT GGCTGTGGTT TGTGACTTTA AACTCTTCCT GTATGATGTC 
GCTGAAGGAA AAGCATCTCA GCCCAGCGTT GTGGTCAGTC AGGTGATTGA CATGAGGGAT 
GAAGAATTTT CTGTGAGTTC AGTGTTGGCT TCTGATGTTA TTCATGCAAG TCGAAAAGAT 
ATACCCTGTA TATTCAGAGT CACAGCTTCC CAGCTCTCAG CATCTGACAG CAGGTGTTCG 
GTCCTAATGC TCGCAGAGAG TGAGAGCGAG AGGAACAAGT GGGTGGGCGT GCTCAACGAG 
CTGCATAAGA TTTTGAAGAA AAGCAAATTC AGAGACCGCT CCGTCTATGT TCCCAAGGAG 
GCTTATGACA GCACTCTGCC CCTCATTAAA ACAACCCAGG CAGCTGCAAT CATAGATCAC 
GAAAGGATAG CTCTGGGCAA TGAAGAAGGA TTATTTGTTG TGCACATCAC CAAAGATGAA 
ATTATTAGAG TTGGTGATAA TAAGAAGATA CATCAGATTG AACTCATTCC AAATGATCAG 
CTTGTTGCTG TGATCTCAGG ACGAAATCGT CATGTACGAC TTTTTCCTAT ATCAGCTTTG 
GACGGACGAG AGACTGATTT TTATAAGCTG GCAGAAACTA AAGGGTGTCA GACCATAACT 
TCGGGCAAAG TGTGCCATGG AGCTCTCACA TGCCTGTGTG TGGCTATGAA AAGGCAGGTC 
CTCTGTTATG AACTATTTCA GAGCAAGACC CGTCACAGGA AATTTAAGGA AATTCAAGTC 
CCATATAACG TGCAATGGAT GGCGATCTTC AGTGAACAGC TCTGCGTGGG ATTCCAGTCG 
GGATTCCTAA GATACCCGTT GAACGGAGAG GGAAGCCCAT ACAGTATGCT CCATTCAAAT 
GACCACACAC TGTCATTTAT TGCCCACCAG CCAATGGATG CCATCTGCGC AGTTGAGATC 
TCCAGTAAAG AGTATCTGCT GTGTTTTAAC AGCATTGGGA TATACACTGA CTGCCAGGGC 
CGGAGATCGA GACAACAGGA GTTGATGTGG CCGTCAAATC CCTCCTCTTG TTGTTACAAC 
GCACCATATC TCTCAGTGTA TAGTGAGAAT GCAGTTGATA TCTTCGATGT GAACTCCATG 
GAATGGATTC AGACTCTCCC ACTCAAAAAG GTTCGACCCT TAAACAGTGA AGGATCATTA 
AACCTTTTAG GGCTGGAGAC AATTAGATTA ATATATTTCA AAAATAAGAT GGCAGAAGGG 
GATGAACTGG TGGTTCCTGA AACATCGGAC AATAGTCGGA AGCAGATGGT TAGAAACATC 
AACAACAAGC GACGCTTCTC CTTCAGGGTC CCAGAAGAGG AGAGGATGCA GCAGAGGAGA 
GAGATGCTAC GAGATCCAGA AATGAGAAAT AAATTAATTT CTAACCCAAC TAATTTTAAC 
CACATAGCAC ACATGGGCCC AGGGGATGGA ATACAGATCC TAAAAGACCT GCCTATGCCA 
GGCTTCCCTT ATCCATCCCC AAATCATCAC TCCGGTCTGA TCTCCTCTCC GATCAACTTT 
GAGCACATCT ATCACATGAC TGTTAATTCT GCTGAAAAAT TTCTCTCTCC TGATTCCATA 
AACCCTGCAT ATTCCCCGTC TCTGCGCTCT GTCCCCGGTA CACCAAGCTT TATGACTCTG 
AGGAACCCTC GGCCTCAGGA AAGCCGGACC GTGTTCAGCG GCTCAGTCAG TATTCCATCC 
ATCACTAAAT CCCGCCCTGA GCCCGGCCGC TCCATGAGCG CCAGCAGCGG CCTTTCGGCA 
AGGTCATCTG CACAGAATGG CAGTGCATTA AAGAGAGAAT TCTCCGGAGG AAGCTATAAT 
GCTAAGTGGC AGCCCATGCC CTCCCCATCA GAGGGCTCCT TGTCTTCCGG AGGCATGGAC 
CAGGGAAGTG ACGCCCCAGC CAGGGACTAT GACGGAGAGG ACTCGGATTC TCCGAGGCAC 
TCCACAGCTT CCAACAGCTC CAACCTCAGC AGCCCGCCCA GCCCAGTGTC ACCTCGGAAG 
ACCAAGAGCC TGTCCCTGGA GAGCACCGAC CGTGGGACCT GGGACCCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_005622621.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622678.1 Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X9, mRNA.

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CloneID	OCe09385
Clone ID Related Accession (Same CDS sequence)	XM_005622621.1
Accession Version	XM_005622621.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase MRCK alpha isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCGTCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTATTGTAG ACAGTACTCC TCTTCCAGCT CACACACCAA CCTTAAGGAA AAAGGGATGT 
CCTGGTTCAA CTGGCTTTCC AGCTAAGCGC AAGACTCATC AGTTTTTTGT CAAATCTTTC 
ACAACACCTA CCAGATGTCA CCAGTGCACC TCTCTGATGG TTGGTTTGAT AAGACAAGGC 
TGTTCCTGTG AGGTGTGCGG ATTCTCATGT CATATAACTT GTGTAAACAA AGCTCCAACT 
GCTTGTCCAG TCCCTCCTGA GCAGACAAAA GGTCCTCTGG GGATAGATCC ACAGAAAGGA 
ATAGGAACAG CCTATGAAGG TCATGTCAGG ATTCCGAAAC CAGCTGGAGT AAAGAAAGGA 
TGGCAGAGAG CCCTGGCTGT GGTTTGTGAC TTTAAACTCT TCCTGTATGA TGTCGCTGAA 
GGAAAAGCAT CTCAGCCCAG CGTTGTGGTC AGTCAGGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTGTT GGCTTCTGAT GTTATTCATG CAAGTCGAAA AGATATACCC 
TGTATATTCA GAGTCACAGC TTCCCAGCTC TCAGCATCTG ACAGCAGGTG TTCGGTCCTA 
ATGCTCGCAG AGAGTGAGAG CGAGAGGAAC AAGTGGGTGG GCGTGCTCAA CGAGCTGCAT 
AAGATTTTGA AGAAAAGCAA ATTCAGAGAC CGCTCCGTCT ATGTTCCCAA GGAGGCTTAT 
GACAGCACTC TGCCCCTCAT TAAAACAACC CAGGCAGCTG CAATCATAGA TCACGAAAGG 
ATAGCTCTGG GCAATGAAGA AGGATTATTT GTTGTGCACA TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ATAATAAGAA GATACATCAG ATTGAACTCA TTCCAAATGA TCAGCTTGTT 
GCTGTGATCT CAGGACGAAA TCGTCATGTA CGACTTTTTC CTATATCAGC TTTGGACGGA 
CGAGAGACTG ATTTTTATAA GCTGGCAGAA ACTAAAGGGT GTCAGACCAT AACTTCGGGC 
AAAGTGTGCC ATGGAGCTCT CACATGCCTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACCCGTCAC AGGAAATTTA AGGAAATTCA AGTCCCATAT 
AACGTGCAAT GGATGGCGAT CTTCAGTGAA CAGCTCTGCG TGGGATTCCA GTCGGGATTC 
CTAAGATACC CGTTGAACGG AGAGGGAAGC CCATACAGTA TGCTCCATTC AAATGACCAC 
ACACTGTCAT TTATTGCCCA CCAGCCAATG GATGCCATCT GCGCAGTTGA GATCTCCAGT 
AAAGAGTATC TGCTGTGTTT TAACAGCATT GGGATATACA CTGACTGCCA GGGCCGGAGA 
TCGAGACAAC AGGAGTTGAT GTGGCCGTCA AATCCCTCCT CTTGTTGTTA CAACGCACCA 
TATCTCTCAG TGTATAGTGA GAATGCAGTT GATATCTTCG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TCCCACTCAA AAAGGTTCGA CCCTTAAACA GTGAAGGATC ATTAAACCTT 
TTAGGGCTGG AGACAATTAG ATTAATATAT TTCAAAAATA AGATGGCAGA AGGGGATGAA 
CTGGTGGTTC CTGAAACATC GGACAATAGT CGGAAGCAGA TGGTTAGAAA CATCAACAAC 
AAGCGACGCT TCTCCTTCAG GGTCCCAGAA GAGGAGAGGA TGCAGCAGAG GAGAGAGATG 
CTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAACC CAACTAATTT TAACCACATA 
GCACACATGG GCCCAGGGGA TGGAATACAG ATCCTAAAAG ACCTGCCTAT GCCAGGCTTC 
CCTTATCCAT CCCCAAATCA TCACTCCGGT CTGATCTCCT CTCCGATCAA CTTTGAGCAC 
ATCTATCACA TGACTGTTAA TTCTGCTGAA AAATTTCTCT CTCCTGATTC CATAAACCCT 
GCATATTCCC CGTCTCTGCG CTCTGTCCCC GGTACACCAA GCTTTATGAC TCTGAGGAAC 
CCTCGGCCTC AGGAAAGCCG GACCGTGTTC AGCGGCTCAG TCAGTATTCC ATCCATCACT 
AAATCCCGCC CTGAGCCCGG CCGCTCCATG AGCGCCAGCA GCGGCCTTTC GGCAAGGTCA 
TCTGCACAGA ATGGCAGTGC ATTAAAGAGA GAATTCTCCG GAGGAAGCTA TAATGCTAAG 
TGGCAGCCCA TGCCCTCCCC ATCAGAGGGC TCCTTGTCTT CCGGAGGCAT GGACCAGGGA 
AGTGACGCCC CAGCCAGGGA CTATGACGGA GAGGACTCGG ATTCTCCGAG GCACTCCACA 
GCTTCCAACA GCTCCAACCT CAGCAGCCCG CCCAGCCCAG TGTCACCTCG GAAGACCAAG 
AGCCTGTCCC TGGAGAGCAC CGACCGTGGG ACCTGGGACC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622678.1
CDS	384..5768
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPASLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKTLKEEIENLRKQIRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIRTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGLCSIEHQQEISKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLYENVSMRNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGARATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRIVDSTPLPAHTPTLRKKGCPGSTGFPAKRKTHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDVAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASDSRCSVLMLAESESERNKWVGVLNELHKILKKSKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHITKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPISALDGRETDFYKLAETKGCQTITSGKVCHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPSNPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRFSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPNHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPAYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNAKWQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGTWDP

Target ORF information:

RefSeq Version	XM_005622621.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622621.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCGTCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTATTGTAG ACAGTACTCC TCTTCCAGCT CACACACCAA CCTTAAGGAA AAAGGGATGT 
CCTGGTTCAA CTGGCTTTCC AGCTAAGCGC AAGACTCATC AGTTTTTTGT CAAATCTTTC 
ACAACACCTA CCAGATGTCA CCAGTGCACC TCTCTGATGG TTGGTTTGAT AAGACAAGGC 
TGTTCCTGTG AGGTGTGCGG ATTCTCATGT CATATAACTT GTGTAAACAA AGCTCCAACT 
GCTTGTCCAG TCCCTCCTGA GCAGACAAAA GGTCCTCTGG GGATAGATCC ACAGAAAGGA 
ATAGGAACAG CCTATGAAGG TCATGTCAGG ATTCCGAAAC CAGCTGGAGT AAAGAAAGGA 
TGGCAGAGAG CCCTGGCTGT GGTTTGTGAC TTTAAACTCT TCCTGTATGA TGTCGCTGAA 
GGAAAAGCAT CTCAGCCCAG CGTTGTGGTC AGTCAGGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTGTT GGCTTCTGAT GTTATTCATG CAAGTCGAAA AGATATACCC 
TGTATATTCA GAGTCACAGC TTCCCAGCTC TCAGCATCTG ACAGCAGGTG TTCGGTCCTA 
ATGCTCGCAG AGAGTGAGAG CGAGAGGAAC AAGTGGGTGG GCGTGCTCAA CGAGCTGCAT 
AAGATTTTGA AGAAAAGCAA ATTCAGAGAC CGCTCCGTCT ATGTTCCCAA GGAGGCTTAT 
GACAGCACTC TGCCCCTCAT TAAAACAACC CAGGCAGCTG CAATCATAGA TCACGAAAGG 
ATAGCTCTGG GCAATGAAGA AGGATTATTT GTTGTGCACA TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ATAATAAGAA GATACATCAG ATTGAACTCA TTCCAAATGA TCAGCTTGTT 
GCTGTGATCT CAGGACGAAA TCGTCATGTA CGACTTTTTC CTATATCAGC TTTGGACGGA 
CGAGAGACTG ATTTTTATAA GCTGGCAGAA ACTAAAGGGT GTCAGACCAT AACTTCGGGC 
AAAGTGTGCC ATGGAGCTCT CACATGCCTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACCCGTCAC AGGAAATTTA AGGAAATTCA AGTCCCATAT 
AACGTGCAAT GGATGGCGAT CTTCAGTGAA CAGCTCTGCG TGGGATTCCA GTCGGGATTC 
CTAAGATACC CGTTGAACGG AGAGGGAAGC CCATACAGTA TGCTCCATTC AAATGACCAC 
ACACTGTCAT TTATTGCCCA CCAGCCAATG GATGCCATCT GCGCAGTTGA GATCTCCAGT 
AAAGAGTATC TGCTGTGTTT TAACAGCATT GGGATATACA CTGACTGCCA GGGCCGGAGA 
TCGAGACAAC AGGAGTTGAT GTGGCCGTCA AATCCCTCCT CTTGTTGTTA CAACGCACCA 
TATCTCTCAG TGTATAGTGA GAATGCAGTT GATATCTTCG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TCCCACTCAA AAAGGTTCGA CCCTTAAACA GTGAAGGATC ATTAAACCTT 
TTAGGGCTGG AGACAATTAG ATTAATATAT TTCAAAAATA AGATGGCAGA AGGGGATGAA 
CTGGTGGTTC CTGAAACATC GGACAATAGT CGGAAGCAGA TGGTTAGAAA CATCAACAAC 
AAGCGACGCT TCTCCTTCAG GGTCCCAGAA GAGGAGAGGA TGCAGCAGAG GAGAGAGATG 
CTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAACC CAACTAATTT TAACCACATA 
GCACACATGG GCCCAGGGGA TGGAATACAG ATCCTAAAAG ACCTGCCTAT GCCAGGCTTC 
CCTTATCCAT CCCCAAATCA TCACTCCGGT CTGATCTCCT CTCCGATCAA CTTTGAGCAC 
ATCTATCACA TGACTGTTAA TTCTGCTGAA AAATTTCTCT CTCCTGATTC CATAAACCCT 
GCATATTCCC CGTCTCTGCG CTCTGTCCCC GGTACACCAA GCTTTATGAC TCTGAGGAAC 
CCTCGGCCTC AGGAAAGCCG GACCGTGTTC AGCGGCTCAG TCAGTATTCC ATCCATCACT 
AAATCCCGCC CTGAGCCCGG CCGCTCCATG AGCGCCAGCA GCGGCCTTTC GGCAAGGTCA 
TCTGCACAGA ATGGCAGTGC ATTAAAGAGA GAATTCTCCG GAGGAAGCTA TAATGCTAAG 
TGGCAGCCCA TGCCCTCCCC ATCAGAGGGC TCCTTGTCTT CCGGAGGCAT GGACCAGGGA 
AGTGACGCCC CAGCCAGGGA CTATGACGGA GAGGACTCGG ATTCTCCGAG GCACTCCACA 
GCTTCCAACA GCTCCAACCT CAGCAGCCCG CCCAGCCCAG TGTCACCTCG GAAGACCAAG 
AGCCTGTCCC TGGAGAGCAC CGACCGTGGG ACCTGGGACC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_005622622.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622679.1 Canis lupus familiaris CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X9, mRNA.

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CloneID	OCe09386
Clone ID Related Accession (Same CDS sequence)	XM_005622622.1 , XM_005622622.2
Accession Version	XM_005622622.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase MRCK alpha isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC GTCCTGTACT CCAGCTAGCA AAGGCAGACG TACCGACCCC 
GTTGAGAGCA CATATGTACG GAATCCGAAC GTCAAGGTTA ACGTCCAGTC AAGGTCCACA 
TCTCCTTCTA CATCTAGTGA AGCTGAGCCA CTTAAGATTG TAGACAGTAC TCCTCTTCCA 
GCTCACACAC CAACCTTAAG GAAAAAGGGA TGTCCTGGTT CAACTGGCTT TCCAGCTAAG 
CGCAAGACTC ATCAGTTTTT TGTCAAATCT TTCACAACAC CTACCAGATG TCACCAGTGC 
ACCTCTCTGA TGGTTGGTTT GATAAGACAA GGCTGTTCCT GTGAGGTGTG CGGATTCTCA 
TGTCATATAA CTTGTGTAAA CAAAGCTCCA ACTGCTTGTC CAGTCCCTCC TGAGCAGACA 
AAAGGTCCTC TGGGGATAGA TCCACAGAAA GGAATAGGAA CAGCCTATGA AGGTCATGTC 
AGGATTCCGA AACCAGCTGG AGTAAAGAAA GGATGGCAGA GAGCCCTGGC TGTGGTTTGT 
GACTTTAAAC TCTTCCTGTA TGATGTCGCT GAAGGAAAAG CATCTCAGCC CAGCGTTGTG 
GTCAGTCAGG TGATTGACAT GAGGGATGAA GAATTTTCTG TGAGTTCAGT GTTGGCTTCT 
GATGTTATTC ATGCAAGTCG AAAAGATATA CCCTGTATAT TCAGAGTCAC AGCTTCCCAG 
CTCTCAGCAT CTGACAGCAG GTGTTCGGTC CTAATGCTCG CAGAGAGTGA GAGCGAGAGG 
AACAAGTGGG TGGGCGTGCT CAACGAGCTG CATAAGATTT TGAAGAAAAG CAAATTCAGA 
GACCGCTCCG TCTATGTTCC CAAGGAGGCT TATGACAGCA CTCTGCCCCT CATTAAAACA 
ACCCAGGCAG CTGCAATCAT AGATCACGAA AGGATAGCTC TGGGCAATGA AGAAGGATTA 
TTTGTTGTGC ACATCACCAA AGATGAAATT ATTAGAGTTG GTGATAATAA GAAGATACAT 
CAGATTGAAC TCATTCCAAA TGATCAGCTT GTTGCTGTGA TCTCAGGACG AAATCGTCAT 
GTACGACTTT TTCCTATATC AGCTTTGGAC GGACGAGAGA CTGATTTTTA TAAGCTGGCA 
GAAACTAAAG GGTGTCAGAC CATAACTTCG GGCAAAGTGT GCCATGGAGC TCTCACATGC 
CTGTGTGTGG CTATGAAAAG GCAGGTCCTC TGTTATGAAC TATTTCAGAG CAAGACCCGT 
CACAGGAAAT TTAAGGAAAT TCAAGTCCCA TATAACGTGC AATGGATGGC GATCTTCAGT 
GAACAGCTCT GCGTGGGATT CCAGTCGGGA TTCCTAAGAT ACCCGTTGAA CGGAGAGGGA 
AGCCCATACA GTATGCTCCA TTCAAATGAC CACACACTGT CATTTATTGC CCACCAGCCA 
ATGGATGCCA TCTGCGCAGT TGAGATCTCC AGTAAAGAGT ATCTGCTGTG TTTTAACAGC 
ATTGGGATAT ACACTGACTG CCAGGGCCGG AGATCGAGAC AACAGGAGTT GATGTGGCCG 
TCAAATCCCT CCTCTTGTTG TTACAACGCA CCATATCTCT CAGTGTATAG TGAGAATGCA 
GTTGATATCT TCGATGTGAA CTCCATGGAA TGGATTCAGA CTCTCCCACT CAAAAAGGTT 
CGACCCTTAA ACAGTGAAGG ATCATTAAAC CTTTTAGGGC TGGAGACAAT TAGATTAATA 
TATTTCAAAA ATAAGATGGC AGAAGGGGAT GAACTGGTGG TTCCTGAAAC ATCGGACAAT 
AGTCGGAAGC AGATGGTTAG AAACATCAAC AACAAGCGAC GCTTCTCCTT CAGGGTCCCA 
GAAGAGGAGA GGATGCAGCA GAGGAGAGAG ATGCTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ACCCAACTAA TTTTAACCAC ATAGCACACA TGGGCCCAGG GGATGGAATA 
CAGATCCTAA AAGACCTGCC TATGAACCCT CGGCCTCAGG AAAGCCGGAC CGTGTTCAGC 
GGCTCAGTCA GTATTCCATC CATCACTAAA TCCCGCCCTG AGCCCGGCCG CTCCATGAGC 
GCCAGCAGCG GCCTTTCGGC AAGGTCATCT GCACAGAATG GCAGTGCATT AAAGAGAGAA 
TTCTCCGGAG GAAGCTATAA TGCTAAGTGG CAGCCCATGC CCTCCCCATC AGAGGGCTCC 
TTGTCTTCCG GAGGCATGGA CCAGGGAAGT GACGCCCCAG CCAGGGACTA TGACGGAGAG 
GACTCGGATT CTCCGAGGCA CTCCACAGCT TCCAACAGCT CCAACCTCAG CAGCCCGCCC 
AGCCCAGTGT CACCTCGGAA GACCAAGAGC CTGTCCCTGG AGAGCACCGA CCGTGGGACC 
TGGGACCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622679.1
CDS	384..5735
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPASLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKTLKEEIENLRKQIRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIRTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGLCSIEHQQEISKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLYENVSMRNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGARATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVESTYVRNPNVKVNVQSRSTSPSTSSEAEPLKIVDSTPLPAHTPTLRKKGCPGSTGFPAKRKTHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDVAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASDSRCSVLMLAESESERNKWVGVLNELHKILKKSKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHITKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPISALDGRETDFYKLAETKGCQTITSGKVCHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPSNPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRFSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNAKWQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGTWDP

Target ORF information:

RefSeq Version	XM_005622622.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622622.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC GTCCTGTACT CCAGCTAGCA AAGGCAGACG TACCGACCCC 
GTTGAGAGCA CATATGTACG GAATCCGAAC GTCAAGGTTA ACGTCCAGTC AAGGTCCACA 
TCTCCTTCTA CATCTAGTGA AGCTGAGCCA CTTAAGATTG TAGACAGTAC TCCTCTTCCA 
GCTCACACAC CAACCTTAAG GAAAAAGGGA TGTCCTGGTT CAACTGGCTT TCCAGCTAAG 
CGCAAGACTC ATCAGTTTTT TGTCAAATCT TTCACAACAC CTACCAGATG TCACCAGTGC 
ACCTCTCTGA TGGTTGGTTT GATAAGACAA GGCTGTTCCT GTGAGGTGTG CGGATTCTCA 
TGTCATATAA CTTGTGTAAA CAAAGCTCCA ACTGCTTGTC CAGTCCCTCC TGAGCAGACA 
AAAGGTCCTC TGGGGATAGA TCCACAGAAA GGAATAGGAA CAGCCTATGA AGGTCATGTC 
AGGATTCCGA AACCAGCTGG AGTAAAGAAA GGATGGCAGA GAGCCCTGGC TGTGGTTTGT 
GACTTTAAAC TCTTCCTGTA TGATGTCGCT GAAGGAAAAG CATCTCAGCC CAGCGTTGTG 
GTCAGTCAGG TGATTGACAT GAGGGATGAA GAATTTTCTG TGAGTTCAGT GTTGGCTTCT 
GATGTTATTC ATGCAAGTCG AAAAGATATA CCCTGTATAT TCAGAGTCAC AGCTTCCCAG 
CTCTCAGCAT CTGACAGCAG GTGTTCGGTC CTAATGCTCG CAGAGAGTGA GAGCGAGAGG 
AACAAGTGGG TGGGCGTGCT CAACGAGCTG CATAAGATTT TGAAGAAAAG CAAATTCAGA 
GACCGCTCCG TCTATGTTCC CAAGGAGGCT TATGACAGCA CTCTGCCCCT CATTAAAACA 
ACCCAGGCAG CTGCAATCAT AGATCACGAA AGGATAGCTC TGGGCAATGA AGAAGGATTA 
TTTGTTGTGC ACATCACCAA AGATGAAATT ATTAGAGTTG GTGATAATAA GAAGATACAT 
CAGATTGAAC TCATTCCAAA TGATCAGCTT GTTGCTGTGA TCTCAGGACG AAATCGTCAT 
GTACGACTTT TTCCTATATC AGCTTTGGAC GGACGAGAGA CTGATTTTTA TAAGCTGGCA 
GAAACTAAAG GGTGTCAGAC CATAACTTCG GGCAAAGTGT GCCATGGAGC TCTCACATGC 
CTGTGTGTGG CTATGAAAAG GCAGGTCCTC TGTTATGAAC TATTTCAGAG CAAGACCCGT 
CACAGGAAAT TTAAGGAAAT TCAAGTCCCA TATAACGTGC AATGGATGGC GATCTTCAGT 
GAACAGCTCT GCGTGGGATT CCAGTCGGGA TTCCTAAGAT ACCCGTTGAA CGGAGAGGGA 
AGCCCATACA GTATGCTCCA TTCAAATGAC CACACACTGT CATTTATTGC CCACCAGCCA 
ATGGATGCCA TCTGCGCAGT TGAGATCTCC AGTAAAGAGT ATCTGCTGTG TTTTAACAGC 
ATTGGGATAT ACACTGACTG CCAGGGCCGG AGATCGAGAC AACAGGAGTT GATGTGGCCG 
TCAAATCCCT CCTCTTGTTG TTACAACGCA CCATATCTCT CAGTGTATAG TGAGAATGCA 
GTTGATATCT TCGATGTGAA CTCCATGGAA TGGATTCAGA CTCTCCCACT CAAAAAGGTT 
CGACCCTTAA ACAGTGAAGG ATCATTAAAC CTTTTAGGGC TGGAGACAAT TAGATTAATA 
TATTTCAAAA ATAAGATGGC AGAAGGGGAT GAACTGGTGG TTCCTGAAAC ATCGGACAAT 
AGTCGGAAGC AGATGGTTAG AAACATCAAC AACAAGCGAC GCTTCTCCTT CAGGGTCCCA 
GAAGAGGAGA GGATGCAGCA GAGGAGAGAG ATGCTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ACCCAACTAA TTTTAACCAC ATAGCACACA TGGGCCCAGG GGATGGAATA 
CAGATCCTAA AAGACCTGCC TATGAACCCT CGGCCTCAGG AAAGCCGGAC CGTGTTCAGC 
GGCTCAGTCA GTATTCCATC CATCACTAAA TCCCGCCCTG AGCCCGGCCG CTCCATGAGC 
GCCAGCAGCG GCCTTTCGGC AAGGTCATCT GCACAGAATG GCAGTGCATT AAAGAGAGAA 
TTCTCCGGAG GAAGCTATAA TGCTAAGTGG CAGCCCATGC CCTCCCCATC AGAGGGCTCC 
TTGTCTTCCG GAGGCATGGA CCAGGGAAGT GACGCCCCAG CCAGGGACTA TGACGGAGAG 
GACTCGGATT CTCCGAGGCA CTCCACAGCT TCCAACAGCT CCAACCTCAG CAGCCCGCCC 
AGCCCAGTGT CACCTCGGAA GACCAAGAGC CTGTCCCTGG AGAGCACCGA CCGTGGGACC 
TGGGACCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_005622622.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622679.1 Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X11, mRNA.

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CloneID	OCe09386
Clone ID Related Accession (Same CDS sequence)	XM_005622622.1 , XM_005622622.2
Accession Version	XM_005622622.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase MRCK alpha isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005622622.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC GTCCTGTACT CCAGCTAGCA AAGGCAGACG TACCGACCCC 
GTTGAGAGCA CATATGTACG GAATCCGAAC GTCAAGGTTA ACGTCCAGTC AAGGTCCACA 
TCTCCTTCTA CATCTAGTGA AGCTGAGCCA CTTAAGATTG TAGACAGTAC TCCTCTTCCA 
GCTCACACAC CAACCTTAAG GAAAAAGGGA TGTCCTGGTT CAACTGGCTT TCCAGCTAAG 
CGCAAGACTC ATCAGTTTTT TGTCAAATCT TTCACAACAC CTACCAGATG TCACCAGTGC 
ACCTCTCTGA TGGTTGGTTT GATAAGACAA GGCTGTTCCT GTGAGGTGTG CGGATTCTCA 
TGTCATATAA CTTGTGTAAA CAAAGCTCCA ACTGCTTGTC CAGTCCCTCC TGAGCAGACA 
AAAGGTCCTC TGGGGATAGA TCCACAGAAA GGAATAGGAA CAGCCTATGA AGGTCATGTC 
AGGATTCCGA AACCAGCTGG AGTAAAGAAA GGATGGCAGA GAGCCCTGGC TGTGGTTTGT 
GACTTTAAAC TCTTCCTGTA TGATGTCGCT GAAGGAAAAG CATCTCAGCC CAGCGTTGTG 
GTCAGTCAGG TGATTGACAT GAGGGATGAA GAATTTTCTG TGAGTTCAGT GTTGGCTTCT 
GATGTTATTC ATGCAAGTCG AAAAGATATA CCCTGTATAT TCAGAGTCAC AGCTTCCCAG 
CTCTCAGCAT CTGACAGCAG GTGTTCGGTC CTAATGCTCG CAGAGAGTGA GAGCGAGAGG 
AACAAGTGGG TGGGCGTGCT CAACGAGCTG CATAAGATTT TGAAGAAAAG CAAATTCAGA 
GACCGCTCCG TCTATGTTCC CAAGGAGGCT TATGACAGCA CTCTGCCCCT CATTAAAACA 
ACCCAGGCAG CTGCAATCAT AGATCACGAA AGGATAGCTC TGGGCAATGA AGAAGGATTA 
TTTGTTGTGC ACATCACCAA AGATGAAATT ATTAGAGTTG GTGATAATAA GAAGATACAT 
CAGATTGAAC TCATTCCAAA TGATCAGCTT GTTGCTGTGA TCTCAGGACG AAATCGTCAT 
GTACGACTTT TTCCTATATC AGCTTTGGAC GGACGAGAGA CTGATTTTTA TAAGCTGGCA 
GAAACTAAAG GGTGTCAGAC CATAACTTCG GGCAAAGTGT GCCATGGAGC TCTCACATGC 
CTGTGTGTGG CTATGAAAAG GCAGGTCCTC TGTTATGAAC TATTTCAGAG CAAGACCCGT 
CACAGGAAAT TTAAGGAAAT TCAAGTCCCA TATAACGTGC AATGGATGGC GATCTTCAGT 
GAACAGCTCT GCGTGGGATT CCAGTCGGGA TTCCTAAGAT ACCCGTTGAA CGGAGAGGGA 
AGCCCATACA GTATGCTCCA TTCAAATGAC CACACACTGT CATTTATTGC CCACCAGCCA 
ATGGATGCCA TCTGCGCAGT TGAGATCTCC AGTAAAGAGT ATCTGCTGTG TTTTAACAGC 
ATTGGGATAT ACACTGACTG CCAGGGCCGG AGATCGAGAC AACAGGAGTT GATGTGGCCG 
TCAAATCCCT CCTCTTGTTG TTACAACGCA CCATATCTCT CAGTGTATAG TGAGAATGCA 
GTTGATATCT TCGATGTGAA CTCCATGGAA TGGATTCAGA CTCTCCCACT CAAAAAGGTT 
CGACCCTTAA ACAGTGAAGG ATCATTAAAC CTTTTAGGGC TGGAGACAAT TAGATTAATA 
TATTTCAAAA ATAAGATGGC AGAAGGGGAT GAACTGGTGG TTCCTGAAAC ATCGGACAAT 
AGTCGGAAGC AGATGGTTAG AAACATCAAC AACAAGCGAC GCTTCTCCTT CAGGGTCCCA 
GAAGAGGAGA GGATGCAGCA GAGGAGAGAG ATGCTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ACCCAACTAA TTTTAACCAC ATAGCACACA TGGGCCCAGG GGATGGAATA 
CAGATCCTAA AAGACCTGCC TATGAACCCT CGGCCTCAGG AAAGCCGGAC CGTGTTCAGC 
GGCTCAGTCA GTATTCCATC CATCACTAAA TCCCGCCCTG AGCCCGGCCG CTCCATGAGC 
GCCAGCAGCG GCCTTTCGGC AAGGTCATCT GCACAGAATG GCAGTGCATT AAAGAGAGAA 
TTCTCCGGAG GAAGCTATAA TGCTAAGTGG CAGCCCATGC CCTCCCCATC AGAGGGCTCC 
TTGTCTTCCG GAGGCATGGA CCAGGGAAGT GACGCCCCAG CCAGGGACTA TGACGGAGAG 
GACTCGGATT CTCCGAGGCA CTCCACAGCT TCCAACAGCT CCAACCTCAG CAGCCCGCCC 
AGCCCAGTGT CACCTCGGAA GACCAAGAGC CTGTCCCTGG AGAGCACCGA CCGTGGGACC 
TGGGACCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622679.1
CDS	384..5735
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPASLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKTLKEEIENLRKQIRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIRTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGLCSIEHQQEISKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLYENVSMRNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGARATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVESTYVRNPNVKVNVQSRSTSPSTSSEAEPLKIVDSTPLPAHTPTLRKKGCPGSTGFPAKRKTHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDVAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASDSRCSVLMLAESESERNKWVGVLNELHKILKKSKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHITKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPISALDGRETDFYKLAETKGCQTITSGKVCHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPSNPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRFSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNAKWQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGTWDP

Target ORF information:

RefSeq Version	XM_005622622.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622622.2

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC GTCCTGTACT CCAGCTAGCA AAGGCAGACG TACCGACCCC 
GTTGAGAGCA CATATGTACG GAATCCGAAC GTCAAGGTTA ACGTCCAGTC AAGGTCCACA 
TCTCCTTCTA CATCTAGTGA AGCTGAGCCA CTTAAGATTG TAGACAGTAC TCCTCTTCCA 
GCTCACACAC CAACCTTAAG GAAAAAGGGA TGTCCTGGTT CAACTGGCTT TCCAGCTAAG 
CGCAAGACTC ATCAGTTTTT TGTCAAATCT TTCACAACAC CTACCAGATG TCACCAGTGC 
ACCTCTCTGA TGGTTGGTTT GATAAGACAA GGCTGTTCCT GTGAGGTGTG CGGATTCTCA 
TGTCATATAA CTTGTGTAAA CAAAGCTCCA ACTGCTTGTC CAGTCCCTCC TGAGCAGACA 
AAAGGTCCTC TGGGGATAGA TCCACAGAAA GGAATAGGAA CAGCCTATGA AGGTCATGTC 
AGGATTCCGA AACCAGCTGG AGTAAAGAAA GGATGGCAGA GAGCCCTGGC TGTGGTTTGT 
GACTTTAAAC TCTTCCTGTA TGATGTCGCT GAAGGAAAAG CATCTCAGCC CAGCGTTGTG 
GTCAGTCAGG TGATTGACAT GAGGGATGAA GAATTTTCTG TGAGTTCAGT GTTGGCTTCT 
GATGTTATTC ATGCAAGTCG AAAAGATATA CCCTGTATAT TCAGAGTCAC AGCTTCCCAG 
CTCTCAGCAT CTGACAGCAG GTGTTCGGTC CTAATGCTCG CAGAGAGTGA GAGCGAGAGG 
AACAAGTGGG TGGGCGTGCT CAACGAGCTG CATAAGATTT TGAAGAAAAG CAAATTCAGA 
GACCGCTCCG TCTATGTTCC CAAGGAGGCT TATGACAGCA CTCTGCCCCT CATTAAAACA 
ACCCAGGCAG CTGCAATCAT AGATCACGAA AGGATAGCTC TGGGCAATGA AGAAGGATTA 
TTTGTTGTGC ACATCACCAA AGATGAAATT ATTAGAGTTG GTGATAATAA GAAGATACAT 
CAGATTGAAC TCATTCCAAA TGATCAGCTT GTTGCTGTGA TCTCAGGACG AAATCGTCAT 
GTACGACTTT TTCCTATATC AGCTTTGGAC GGACGAGAGA CTGATTTTTA TAAGCTGGCA 
GAAACTAAAG GGTGTCAGAC CATAACTTCG GGCAAAGTGT GCCATGGAGC TCTCACATGC 
CTGTGTGTGG CTATGAAAAG GCAGGTCCTC TGTTATGAAC TATTTCAGAG CAAGACCCGT 
CACAGGAAAT TTAAGGAAAT TCAAGTCCCA TATAACGTGC AATGGATGGC GATCTTCAGT 
GAACAGCTCT GCGTGGGATT CCAGTCGGGA TTCCTAAGAT ACCCGTTGAA CGGAGAGGGA 
AGCCCATACA GTATGCTCCA TTCAAATGAC CACACACTGT CATTTATTGC CCACCAGCCA 
ATGGATGCCA TCTGCGCAGT TGAGATCTCC AGTAAAGAGT ATCTGCTGTG TTTTAACAGC 
ATTGGGATAT ACACTGACTG CCAGGGCCGG AGATCGAGAC AACAGGAGTT GATGTGGCCG 
TCAAATCCCT CCTCTTGTTG TTACAACGCA CCATATCTCT CAGTGTATAG TGAGAATGCA 
GTTGATATCT TCGATGTGAA CTCCATGGAA TGGATTCAGA CTCTCCCACT CAAAAAGGTT 
CGACCCTTAA ACAGTGAAGG ATCATTAAAC CTTTTAGGGC TGGAGACAAT TAGATTAATA 
TATTTCAAAA ATAAGATGGC AGAAGGGGAT GAACTGGTGG TTCCTGAAAC ATCGGACAAT 
AGTCGGAAGC AGATGGTTAG AAACATCAAC AACAAGCGAC GCTTCTCCTT CAGGGTCCCA 
GAAGAGGAGA GGATGCAGCA GAGGAGAGAG ATGCTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ACCCAACTAA TTTTAACCAC ATAGCACACA TGGGCCCAGG GGATGGAATA 
CAGATCCTAA AAGACCTGCC TATGAACCCT CGGCCTCAGG AAAGCCGGAC CGTGTTCAGC 
GGCTCAGTCA GTATTCCATC CATCACTAAA TCCCGCCCTG AGCCCGGCCG CTCCATGAGC 
GCCAGCAGCG GCCTTTCGGC AAGGTCATCT GCACAGAATG GCAGTGCATT AAAGAGAGAA 
TTCTCCGGAG GAAGCTATAA TGCTAAGTGG CAGCCCATGC CCTCCCCATC AGAGGGCTCC 
TTGTCTTCCG GAGGCATGGA CCAGGGAAGT GACGCCCCAG CCAGGGACTA TGACGGAGAG 
GACTCGGATT CTCCGAGGCA CTCCACAGCT TCCAACAGCT CCAACCTCAG CAGCCCGCCC 
AGCCCAGTGT CACCTCGGAA GACCAAGAGC CTGTCCCTGG AGAGCACCGA CCGTGGGACC 
TGGGACCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_005622623.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622680.1 Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X12, mRNA.

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CloneID	OCe09387
Clone ID Related Accession (Same CDS sequence)	XM_005622623.1
Accession Version	XM_005622623.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase MRCK alpha isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAATTGTA GACAGTACTC CTCTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG TCCTGGTTCA ACTGGCTTTC CAGCTAAGCG CAAGACTCAT 
CAGTTTTTTG TCAAATCTTT CACAACACCT ACCAGATGTC ACCAGTGCAC CTCTCTGATG 
GTTGGTTTGA TAAGACAAGG CTGTTCCTGT GAGGTGTGCG GATTCTCATG TCATATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTCCCTCCTG AGCAGACAAA AGGTCCTCTG 
GGGATAGATC CACAGAAAGG AATAGGAACA GCCTATGAAG GTCATGTCAG GATTCCGAAA 
CCAGCTGGAG TAAAGAAAGG ATGGCAGAGA GCCCTGGCTG TGGTTTGTGA CTTTAAACTC 
TTCCTGTATG ATGTCGCTGA AGGAAAAGCA TCTCAGCCCA GCGTTGTGGT CAGTCAGGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTGT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTC AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
GACAGCAGGT GTTCGGTCCT AATGCTCGCA GAGAGTGAGA GCGAGAGGAA CAAGTGGGTG 
GGCGTGCTCA ACGAGCTGCA TAAGATTTTG AAGAAAAGCA AATTCAGAGA CCGCTCCGTC 
TATGTTCCCA AGGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCACGAAAG GATAGCTCTG GGCAATGAAG AAGGATTATT TGTTGTGCAC 
ATCACCAAAG ATGAAATTAT TAGAGTTGGT GATAATAAGA AGATACATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATATCAG CTTTGGACGG ACGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGG 
TGTCAGACCA TAACTTCGGG CAAAGTGTGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGGAAATTT 
AAGGAAATTC AAGTCCCATA TAACGTGCAA TGGATGGCGA TCTTCAGTGA ACAGCTCTGC 
GTGGGATTCC AGTCGGGATT CCTAAGATAC CCGTTGAACG GAGAGGGAAG CCCATACAGT 
ATGCTCCATT CAAATGACCA CACACTGTCA TTTATTGCCC ACCAGCCAAT GGATGCCATC 
TGCGCAGTTG AGATCTCCAG TAAAGAGTAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGGAG ATCGAGACAA CAGGAGTTGA TGTGGCCGTC AAATCCCTCC 
TCTTGTTGTT ACAACGCACC ATATCTCTCA GTGTATAGTG AGAATGCAGT TGATATCTTC 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG ACCCTTAAAC 
AGTGAAGGAT CATTAAACCT TTTAGGGCTG GAGACAATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTGGTGGTT CCTGAAACAT CGGACAATAG TCGGAAGCAG 
ATGGTTAGAA ACATCAACAA CAAGCGACGC TTCTCCTTCA GGGTCCCAGA AGAGGAGAGG 
ATGCAGCAGA GGAGAGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGCCCAGGGG ATGGAATACA GATCCTAAAA 
GACCTGCCTA TGCCAGGCTT CCCTTATCCA TCCCCAAATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAGCA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGCATATTCC CCGTCTCTGC GCTCTGTCCC CGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGCC GGACCGTGTT CAGCGGCTCA 
GTCAGTATTC CATCCATCAC TAAATCCCGC CCTGAGCCCG GCCGCTCCAT GAGCGCCAGC 
AGCGGCCTTT CGGCAAGGTC ATCTGCACAG AATGGCAGTG CATTAAAGAG AGAATTCTCC 
GGAGGAAGCT ATAATGCTAA GTGGCAGCCC ATGCCCTCCC CATCAGAGGG CTCCTTGTCT 
TCCGGAGGCA TGGACCAGGG AAGTGACGCC CCAGCCAGGG ACTATGACGG AGAGGACTCG 
GATTCTCCGA GGCACTCCAC AGCTTCCAAC AGCTCCAACC TCAGCAGCCC GCCCAGCCCA 
GTGTCACCTC GGAAGACCAA GAGCCTGTCC CTGGAGAGCA CCGACCGTGG GACCTGGGAC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622680.1
CDS	384..5729
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPASLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKTLKEEIENLRKQIRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIRTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGLCSIEHQQEISKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLYENVSMRNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGARATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEIVDSTPLPAHTPTLRKKGCPGSTGFPAKRKTHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDVAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASDSRCSVLMLAESESERNKWVGVLNELHKILKKSKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHITKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPISALDGRETDFYKLAETKGCQTITSGKVCHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPSNPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRFSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPNHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPAYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNAKWQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGTWDP

Target ORF information:

RefSeq Version	XM_005622623.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622623.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAATTGTA GACAGTACTC CTCTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG TCCTGGTTCA ACTGGCTTTC CAGCTAAGCG CAAGACTCAT 
CAGTTTTTTG TCAAATCTTT CACAACACCT ACCAGATGTC ACCAGTGCAC CTCTCTGATG 
GTTGGTTTGA TAAGACAAGG CTGTTCCTGT GAGGTGTGCG GATTCTCATG TCATATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTCCCTCCTG AGCAGACAAA AGGTCCTCTG 
GGGATAGATC CACAGAAAGG AATAGGAACA GCCTATGAAG GTCATGTCAG GATTCCGAAA 
CCAGCTGGAG TAAAGAAAGG ATGGCAGAGA GCCCTGGCTG TGGTTTGTGA CTTTAAACTC 
TTCCTGTATG ATGTCGCTGA AGGAAAAGCA TCTCAGCCCA GCGTTGTGGT CAGTCAGGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTGT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTC AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
GACAGCAGGT GTTCGGTCCT AATGCTCGCA GAGAGTGAGA GCGAGAGGAA CAAGTGGGTG 
GGCGTGCTCA ACGAGCTGCA TAAGATTTTG AAGAAAAGCA AATTCAGAGA CCGCTCCGTC 
TATGTTCCCA AGGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCACGAAAG GATAGCTCTG GGCAATGAAG AAGGATTATT TGTTGTGCAC 
ATCACCAAAG ATGAAATTAT TAGAGTTGGT GATAATAAGA AGATACATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATATCAG CTTTGGACGG ACGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGG 
TGTCAGACCA TAACTTCGGG CAAAGTGTGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGGAAATTT 
AAGGAAATTC AAGTCCCATA TAACGTGCAA TGGATGGCGA TCTTCAGTGA ACAGCTCTGC 
GTGGGATTCC AGTCGGGATT CCTAAGATAC CCGTTGAACG GAGAGGGAAG CCCATACAGT 
ATGCTCCATT CAAATGACCA CACACTGTCA TTTATTGCCC ACCAGCCAAT GGATGCCATC 
TGCGCAGTTG AGATCTCCAG TAAAGAGTAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGGAG ATCGAGACAA CAGGAGTTGA TGTGGCCGTC AAATCCCTCC 
TCTTGTTGTT ACAACGCACC ATATCTCTCA GTGTATAGTG AGAATGCAGT TGATATCTTC 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG ACCCTTAAAC 
AGTGAAGGAT CATTAAACCT TTTAGGGCTG GAGACAATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTGGTGGTT CCTGAAACAT CGGACAATAG TCGGAAGCAG 
ATGGTTAGAA ACATCAACAA CAAGCGACGC TTCTCCTTCA GGGTCCCAGA AGAGGAGAGG 
ATGCAGCAGA GGAGAGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGCCCAGGGG ATGGAATACA GATCCTAAAA 
GACCTGCCTA TGCCAGGCTT CCCTTATCCA TCCCCAAATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAGCA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGCATATTCC CCGTCTCTGC GCTCTGTCCC CGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGCC GGACCGTGTT CAGCGGCTCA 
GTCAGTATTC CATCCATCAC TAAATCCCGC CCTGAGCCCG GCCGCTCCAT GAGCGCCAGC 
AGCGGCCTTT CGGCAAGGTC ATCTGCACAG AATGGCAGTG CATTAAAGAG AGAATTCTCC 
GGAGGAAGCT ATAATGCTAA GTGGCAGCCC ATGCCCTCCC CATCAGAGGG CTCCTTGTCT 
TCCGGAGGCA TGGACCAGGG AAGTGACGCC CCAGCCAGGG ACTATGACGG AGAGGACTCG 
GATTCTCCGA GGCACTCCAC AGCTTCCAAC AGCTCCAACC TCAGCAGCCC GCCCAGCCCA 
GTGTCACCTC GGAAGACCAA GAGCCTGTCC CTGGAGAGCA CCGACCGTGG GACCTGGGAC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_005622624.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622681.1 Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X13, mRNA.

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CloneID	OCe09388
Clone ID Related Accession (Same CDS sequence)	XM_005622624.1
Accession Version	XM_005622624.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase MRCK alpha isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGCTG GAAGTTCATA CAGAAGCTGT GGCTGCTGAA 
GCATCTAAAG ATAGGAAACT GCGTGAACAG AGTGAGCATT ATTCTAAGCA ACTGGAAAAT 
GAATTGGAGG GTCTAAAGCA GAAACAAATT AGTTACTCCC CAGGACTGTG CAGCATAGAA 
CACCAGCAGG AGATATCCAA ACTAAAGACG GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAGGAATTGT CTAAAAGAGA AGGAATACAT GCAAATGAAA TTAAAAATCT GAAGAAAGAA 
CTTCATGATT CAGAAGGCCA GCAACTTGCT CTCAACAAGG AAATTATGAT TTTGAAAGAC 
AAATTAGAGA AAAACAGAAG AGAGAGTCAA AGTGAAAGGG AAGAGTTTGA AAATGAATTC 
AAACAACAAT ATGAACGAGA AAAAGTATTA TTAACAGAAG AAAATAAAAA ACTGACAAGT 
GAACTTGATA AGCTCACCAC TTTGTATGAA AATGTAAGTA TGCGCAACCA GCAGTTAGAA 
GAAGAGGTGA AAGACCTAGC AGACAAGAAA GAATCAGTTG CACATTGGGA AGCCCAAATC 
ACAGAAATAA TTCAGTGGGT CAGTGATGAA AAGGATGCAC GAGGGTATCT CCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAAGCT TTGAGGAATT CCAGCTTGGG TGCACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCAAAATTGG ATATGTCAGC TAGACTGGAG 
TTGCAGTCAG CTCTGGATGC AGAAATAAGA GCTAAACAGG CCATCCAAGA AGAACTGAAT 
AAAGTTAAAG CATCTAACAT CATCACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAGCTCC TCTCAGAAAT CGAACAACTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AGATTCCTTT TCTTCTTCCT CATCTTCACT GATTGATTTT 
TTGGATGACC GCTCTCCGTC CTGTACTCCA GCTAGCAAAG GCAGACGTAC CGACCCCGTT 
GAGAGCACAT ATGTACGGAA TCCGAACGTC AAGGTTAACG TCCAGTCAAG GTCCACATCT 
CCTTCTACAT CTAGTGAAGC TGAGCCACTT AAGATTGTAG ACAGTACTCC TCTTCCAGCT 
CACACACCAA CCTTAAGGAA AAAGGGATGT CCTGGTTCAA CTGGCTTTCC AGCTAAGCGC 
AAGACTCATC AGTTTTTTGT CAAATCTTTC ACAACACCTA CCAGATGTCA CCAGTGCACC 
TCTCTGATGG TTGGTTTGAT AAGACAAGGC TGTTCCTGTG AGGTGTGCGG ATTCTCATGT 
CATATAACTT GTGTAAACAA AGCTCCAACT GCTTGTCCAG TCCCTCCTGA GCAGACAAAA 
GGTCCTCTGG GGATAGATCC ACAGAAAGGA ATAGGAACAG CCTATGAAGG TCATGTCAGG 
ATTCCGAAAC CAGCTGGAGT AAAGAAAGGA TGGCAGAGAG CCCTGGCTGT GGTTTGTGAC 
TTTAAACTCT TCCTGTATGA TGTCGCTGAA GGAAAAGCAT CTCAGCCCAG CGTTGTGGTC 
AGTCAGGTGA TTGACATGAG GGATGAAGAA TTTTCTGTGA GTTCAGTGTT GGCTTCTGAT 
GTTATTCATG CAAGTCGAAA AGATATACCC TGTATATTCA GAGTCACAGC TTCCCAGCTC 
TCAGCATCTG ACAGCAGGTG TTCGGTCCTA ATGCTCGCAG AGAGTGAGAG CGAGAGGAAC 
AAGTGGGTGG GCGTGCTCAA CGAGCTGCAT AAGATTTTGA AGAAAAGCAA ATTCAGAGAC 
CGCTCCGTCT ATGTTCCCAA GGAGGCTTAT GACAGCACTC TGCCCCTCAT TAAAACAACC 
CAGGCAGCTG CAATCATAGA TCACGAAAGG ATAGCTCTGG GCAATGAAGA AGGATTATTT 
GTTGTGCACA TCACCAAAGA TGAAATTATT AGAGTTGGTG ATAATAAGAA GATACATCAG 
ATTGAACTCA TTCCAAATGA TCAGCTTGTT GCTGTGATCT CAGGACGAAA TCGTCATGTA 
CGACTTTTTC CTATATCAGC TTTGGACGGA CGAGAGACTG ATTTTTATAA GCTGGCAGAA 
ACTAAAGGGT GTCAGACCAT AACTTCGGGC AAAGTGTGCC ATGGAGCTCT CACATGCCTG 
TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT TATGAACTAT TTCAGAGCAA GACCCGTCAC 
AGGAAATTTA AGGAAATTCA AGTCCCATAT AACGTGCAAT GGATGGCGAT CTTCAGTGAA 
CAGCTCTGCG TGGGATTCCA GTCGGGATTC CTAAGATACC CGTTGAACGG AGAGGGAAGC 
CCATACAGTA TGCTCCATTC AAATGACCAC ACACTGTCAT TTATTGCCCA CCAGCCAATG 
GATGCCATCT GCGCAGTTGA GATCTCCAGT AAAGAGTATC TGCTGTGTTT TAACAGCATT 
GGGATATACA CTGACTGCCA GGGCCGGAGA TCGAGACAAC AGGAGTTGAT GTGGCCGTCA 
AATCCCTCCT CTTGTTGTTA CAACGCACCA TATCTCTCAG TGTATAGTGA GAATGCAGTT 
GATATCTTCG ATGTGAACTC CATGGAATGG ATTCAGACTC TCCCACTCAA AAAGGTTCGA 
CCCTTAAACA GTGAAGGATC ATTAAACCTT TTAGGGCTGG AGACAATTAG ATTAATATAT 
TTCAAAAATA AGATGGCAGA AGGGGATGAA CTGGTGGTTC CTGAAACATC GGACAATAGT 
CGGAAGCAGA TGGTTAGAAA CATCAACAAC AAGCGACGCT TCTCCTTCAG GGTCCCAGAA 
GAGGAGAGGA TGCAGCAGAG GAGAGAGATG CTACGAGATC CAGAAATGAG AAATAAATTA 
ATTTCTAACC CAACTAATTT TAACCACATA GCACACATGG GCCCAGGGGA TGGAATACAG 
ATCCTAAAAG ACCTGCCTAT GCCAGGCTTC CCTTATCCAT CCCCAAATCA TCACTCCGGT 
CTGATCTCCT CTCCGATCAA CTTTGAGCAC ATCTATCACA TGACTGTTAA TTCTGCTGAA 
AAATTTCTCT CTCCTGATTC CATAAACCCT GCATATTCCC CGTCTCTGCG CTCTGTCCCC 
GGTACACCAA GCTTTATGAC TCTGAGGAAC CCTCGGCCTC AGGAAAGCCG GACCGTGTTC 
AGCGGCTCAG TCAGTATTCC ATCCATCACT AAATCCCGCC CTGAGCCCGG CCGCTCCATG 
AGCGCCAGCA GCGGCCTTTC GGCAAGGTCA TCTGCACAGA ATGGCAGTGC ATTAAAGAGA 
GAATTCTCCG GAGGAAGCTA TAATGCTAAG TGGCAGCCCA TGCCCTCCCC ATCAGAGGGC 
TCCTTGTCTT CCGGAGGCAT GGACCAGGGA AGTGACGCCC CAGCCAGGGA CTATGACGGA 
GAGGACTCGG ATTCTCCGAG GCACTCCACA GCTTCCAACA GCTCCAACCT CAGCAGCCCG 
CCCAGCCCAG TGTCACCTCG GAAGACCAAG AGCCTGTCCC TGGAGAGCAC CGACCGTGGG 
ACCTGGGACC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622681.1
CDS	384..5678
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPASLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKTLKEEIENLRKQIRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIRTLQQEREELNKLEVHTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGLCSIEHQQEISKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLYENVSMRNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGARATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVESTYVRNPNVKVNVQSRSTSPSTSSEAEPLKIVDSTPLPAHTPTLRKKGCPGSTGFPAKRKTHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDVAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASDSRCSVLMLAESESERNKWVGVLNELHKILKKSKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHITKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPISALDGRETDFYKLAETKGCQTITSGKVCHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPSNPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRFSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPNHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPAYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNAKWQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGTWDP

Target ORF information:

RefSeq Version	XM_005622624.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622624.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGCTG GAAGTTCATA CAGAAGCTGT GGCTGCTGAA 
GCATCTAAAG ATAGGAAACT GCGTGAACAG AGTGAGCATT ATTCTAAGCA ACTGGAAAAT 
GAATTGGAGG GTCTAAAGCA GAAACAAATT AGTTACTCCC CAGGACTGTG CAGCATAGAA 
CACCAGCAGG AGATATCCAA ACTAAAGACG GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAGGAATTGT CTAAAAGAGA AGGAATACAT GCAAATGAAA TTAAAAATCT GAAGAAAGAA 
CTTCATGATT CAGAAGGCCA GCAACTTGCT CTCAACAAGG AAATTATGAT TTTGAAAGAC 
AAATTAGAGA AAAACAGAAG AGAGAGTCAA AGTGAAAGGG AAGAGTTTGA AAATGAATTC 
AAACAACAAT ATGAACGAGA AAAAGTATTA TTAACAGAAG AAAATAAAAA ACTGACAAGT 
GAACTTGATA AGCTCACCAC TTTGTATGAA AATGTAAGTA TGCGCAACCA GCAGTTAGAA 
GAAGAGGTGA AAGACCTAGC AGACAAGAAA GAATCAGTTG CACATTGGGA AGCCCAAATC 
ACAGAAATAA TTCAGTGGGT CAGTGATGAA AAGGATGCAC GAGGGTATCT CCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAAGCT TTGAGGAATT CCAGCTTGGG TGCACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCAAAATTGG ATATGTCAGC TAGACTGGAG 
TTGCAGTCAG CTCTGGATGC AGAAATAAGA GCTAAACAGG CCATCCAAGA AGAACTGAAT 
AAAGTTAAAG CATCTAACAT CATCACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAGCTCC TCTCAGAAAT CGAACAACTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AGATTCCTTT TCTTCTTCCT CATCTTCACT GATTGATTTT 
TTGGATGACC GCTCTCCGTC CTGTACTCCA GCTAGCAAAG GCAGACGTAC CGACCCCGTT 
GAGAGCACAT ATGTACGGAA TCCGAACGTC AAGGTTAACG TCCAGTCAAG GTCCACATCT 
CCTTCTACAT CTAGTGAAGC TGAGCCACTT AAGATTGTAG ACAGTACTCC TCTTCCAGCT 
CACACACCAA CCTTAAGGAA AAAGGGATGT CCTGGTTCAA CTGGCTTTCC AGCTAAGCGC 
AAGACTCATC AGTTTTTTGT CAAATCTTTC ACAACACCTA CCAGATGTCA CCAGTGCACC 
TCTCTGATGG TTGGTTTGAT AAGACAAGGC TGTTCCTGTG AGGTGTGCGG ATTCTCATGT 
CATATAACTT GTGTAAACAA AGCTCCAACT GCTTGTCCAG TCCCTCCTGA GCAGACAAAA 
GGTCCTCTGG GGATAGATCC ACAGAAAGGA ATAGGAACAG CCTATGAAGG TCATGTCAGG 
ATTCCGAAAC CAGCTGGAGT AAAGAAAGGA TGGCAGAGAG CCCTGGCTGT GGTTTGTGAC 
TTTAAACTCT TCCTGTATGA TGTCGCTGAA GGAAAAGCAT CTCAGCCCAG CGTTGTGGTC 
AGTCAGGTGA TTGACATGAG GGATGAAGAA TTTTCTGTGA GTTCAGTGTT GGCTTCTGAT 
GTTATTCATG CAAGTCGAAA AGATATACCC TGTATATTCA GAGTCACAGC TTCCCAGCTC 
TCAGCATCTG ACAGCAGGTG TTCGGTCCTA ATGCTCGCAG AGAGTGAGAG CGAGAGGAAC 
AAGTGGGTGG GCGTGCTCAA CGAGCTGCAT AAGATTTTGA AGAAAAGCAA ATTCAGAGAC 
CGCTCCGTCT ATGTTCCCAA GGAGGCTTAT GACAGCACTC TGCCCCTCAT TAAAACAACC 
CAGGCAGCTG CAATCATAGA TCACGAAAGG ATAGCTCTGG GCAATGAAGA AGGATTATTT 
GTTGTGCACA TCACCAAAGA TGAAATTATT AGAGTTGGTG ATAATAAGAA GATACATCAG 
ATTGAACTCA TTCCAAATGA TCAGCTTGTT GCTGTGATCT CAGGACGAAA TCGTCATGTA 
CGACTTTTTC CTATATCAGC TTTGGACGGA CGAGAGACTG ATTTTTATAA GCTGGCAGAA 
ACTAAAGGGT GTCAGACCAT AACTTCGGGC AAAGTGTGCC ATGGAGCTCT CACATGCCTG 
TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT TATGAACTAT TTCAGAGCAA GACCCGTCAC 
AGGAAATTTA AGGAAATTCA AGTCCCATAT AACGTGCAAT GGATGGCGAT CTTCAGTGAA 
CAGCTCTGCG TGGGATTCCA GTCGGGATTC CTAAGATACC CGTTGAACGG AGAGGGAAGC 
CCATACAGTA TGCTCCATTC AAATGACCAC ACACTGTCAT TTATTGCCCA CCAGCCAATG 
GATGCCATCT GCGCAGTTGA GATCTCCAGT AAAGAGTATC TGCTGTGTTT TAACAGCATT 
GGGATATACA CTGACTGCCA GGGCCGGAGA TCGAGACAAC AGGAGTTGAT GTGGCCGTCA 
AATCCCTCCT CTTGTTGTTA CAACGCACCA TATCTCTCAG TGTATAGTGA GAATGCAGTT 
GATATCTTCG ATGTGAACTC CATGGAATGG ATTCAGACTC TCCCACTCAA AAAGGTTCGA 
CCCTTAAACA GTGAAGGATC ATTAAACCTT TTAGGGCTGG AGACAATTAG ATTAATATAT 
TTCAAAAATA AGATGGCAGA AGGGGATGAA CTGGTGGTTC CTGAAACATC GGACAATAGT 
CGGAAGCAGA TGGTTAGAAA CATCAACAAC AAGCGACGCT TCTCCTTCAG GGTCCCAGAA 
GAGGAGAGGA TGCAGCAGAG GAGAGAGATG CTACGAGATC CAGAAATGAG AAATAAATTA 
ATTTCTAACC CAACTAATTT TAACCACATA GCACACATGG GCCCAGGGGA TGGAATACAG 
ATCCTAAAAG ACCTGCCTAT GCCAGGCTTC CCTTATCCAT CCCCAAATCA TCACTCCGGT 
CTGATCTCCT CTCCGATCAA CTTTGAGCAC ATCTATCACA TGACTGTTAA TTCTGCTGAA 
AAATTTCTCT CTCCTGATTC CATAAACCCT GCATATTCCC CGTCTCTGCG CTCTGTCCCC 
GGTACACCAA GCTTTATGAC TCTGAGGAAC CCTCGGCCTC AGGAAAGCCG GACCGTGTTC 
AGCGGCTCAG TCAGTATTCC ATCCATCACT AAATCCCGCC CTGAGCCCGG CCGCTCCATG 
AGCGCCAGCA GCGGCCTTTC GGCAAGGTCA TCTGCACAGA ATGGCAGTGC ATTAAAGAGA 
GAATTCTCCG GAGGAAGCTA TAATGCTAAG TGGCAGCCCA TGCCCTCCCC ATCAGAGGGC 
TCCTTGTCTT CCGGAGGCAT GGACCAGGGA AGTGACGCCC CAGCCAGGGA CTATGACGGA 
GAGGACTCGG ATTCTCCGAG GCACTCCACA GCTTCCAACA GCTCCAACCT CAGCAGCCCG 
CCCAGCCCAG TGTCACCTCG GAAGACCAAG AGCCTGTCCC TGGAGAGCAC CGACCGTGGG 
ACCTGGGACC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_005622625.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622682.1 Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X15, mRNA.

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CloneID	OCe09389
Clone ID Related Accession (Same CDS sequence)	XM_005622625.1
Accession Version	XM_005622625.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase MRCK alpha isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGCTA GCAAAGGCAG ACGTATTGTA GACAGTACTC CTCTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG TCCTGGTTCA ACTGGCTTTC CAGCTAAGCG CAAGACTCAT 
CAGTTTTTTG TCAAATCTTT CACAACACCT ACCAGATGTC ACCAGTGCAC CTCTCTGATG 
GTTGGTTTGA TAAGACAAGG CTGTTCCTGT GAGGTGTGCG GATTCTCATG TCATATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTCCCTCCTG AGCAGACAAA AGGTCCTCTG 
GGGATAGATC CACAGAAAGG AATAGGAACA GCCTATGAAG GTCATGTCAG GATTCCGAAA 
CCAGCTGGAG TAAAGAAAGG ATGGCAGAGA GCCCTGGCTG TGGTTTGTGA CTTTAAACTC 
TTCCTGTATG ATGTCGCTGA AGGAAAAGCA TCTCAGCCCA GCGTTGTGGT CAGTCAGGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTGT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTC AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
GACAGCAGGT GTTCGGTCCT AATGCTCGCA GAGAGTGAGA GCGAGAGGAA CAAGTGGGTG 
GGCGTGCTCA ACGAGCTGCA TAAGATTTTG AAGAAAAGCA AATTCAGAGA CCGCTCCGTC 
TATGTTCCCA AGGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCACGAAAG GATAGCTCTG GGCAATGAAG AAGGATTATT TGTTGTGCAC 
ATCACCAAAG ATGAAATTAT TAGAGTTGGT GATAATAAGA AGATACATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATATCAG CTTTGGACGG ACGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGG 
TGTCAGACCA TAACTTCGGG CAAAGTGTGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGGAAATTT 
AAGGAAATTC AAGTCCCATA TAACGTGCAA TGGATGGCGA TCTTCAGTGA ACAGCTCTGC 
GTGGGATTCC AGTCGGGATT CCTAAGATAC CCGTTGAACG GAGAGGGAAG CCCATACAGT 
ATGCTCCATT CAAATGACCA CACACTGTCA TTTATTGCCC ACCAGCCAAT GGATGCCATC 
TGCGCAGTTG AGATCTCCAG TAAAGAGTAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGGAG ATCGAGACAA CAGGAGTTGA TGTGGCCGTC AAATCCCTCC 
TCTTGTTGTT ACAACGCACC ATATCTCTCA GTGTATAGTG AGAATGCAGT TGATATCTTC 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG ACCCTTAAAC 
AGTGAAGGAT CATTAAACCT TTTAGGGCTG GAGACAATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTGGTGGTT CCTGAAACAT CGGACAATAG TCGGAAGCAG 
ATGGTTAGAA ACATCAACAA CAAGCGACGC TTCTCCTTCA GGGTCCCAGA AGAGGAGAGG 
ATGCAGCAGA GGAGAGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGCCCAGGGG ATGGAATACA GATCCTAAAA 
GACCTGCCTA TGCCAGGCTT CCCTTATCCA TCCCCAAATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAGCA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGCATATTCC CCGTCTCTGC GCTCTGTCCC CGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGCC GGACCGTGTT CAGCGGCTCA 
GTCAGTATTC CATCCATCAC TAAATCCCGC CCTGAGCCCG GCCGCTCCAT GAGCGCCAGC 
AGCGGCCTTT CGGCAAGGTC ATCTGCACAG AATGGCAGTG CATTAAAGAG AGAATTCTCC 
GGAGGAAGCT ATAATGCTAA GTGGCAGCCC ATGCCCTCCC CATCAGAGGG CTCCTTGTCT 
TCCGGAGGCA TGGACCAGGG AAGTGACGCC CCAGCCAGGG ACTATGACGG AGAGGACTCG 
GATTCTCCGA GGCACTCCAC AGCTTCCAAC AGCTCCAACC TCAGCAGCCC GCCCAGCCCA 
GTGTCACCTC GGAAGACCAA GAGCCTGTCC CTGGAGAGCA CCGACCGTGG GACCTGGGAC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622682.1
CDS	384..5669
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPASLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKTLKEEIENLRKQIRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIRTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGLCSIEHQQEISKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLYENVSMRNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGARATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKASKGRRIVDSTPLPAHTPTLRKKGCPGSTGFPAKRKTHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDVAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASDSRCSVLMLAESESERNKWVGVLNELHKILKKSKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHITKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPISALDGRETDFYKLAETKGCQTITSGKVCHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPSNPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRFSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPNHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPAYSPSLRSVPGTPSFMTLRNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNAKWQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGTWDP

Target ORF information:

RefSeq Version	XM_005622625.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622625.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGCTA GCAAAGGCAG ACGTATTGTA GACAGTACTC CTCTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG TCCTGGTTCA ACTGGCTTTC CAGCTAAGCG CAAGACTCAT 
CAGTTTTTTG TCAAATCTTT CACAACACCT ACCAGATGTC ACCAGTGCAC CTCTCTGATG 
GTTGGTTTGA TAAGACAAGG CTGTTCCTGT GAGGTGTGCG GATTCTCATG TCATATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTCCCTCCTG AGCAGACAAA AGGTCCTCTG 
GGGATAGATC CACAGAAAGG AATAGGAACA GCCTATGAAG GTCATGTCAG GATTCCGAAA 
CCAGCTGGAG TAAAGAAAGG ATGGCAGAGA GCCCTGGCTG TGGTTTGTGA CTTTAAACTC 
TTCCTGTATG ATGTCGCTGA AGGAAAAGCA TCTCAGCCCA GCGTTGTGGT CAGTCAGGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTGT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTC AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
GACAGCAGGT GTTCGGTCCT AATGCTCGCA GAGAGTGAGA GCGAGAGGAA CAAGTGGGTG 
GGCGTGCTCA ACGAGCTGCA TAAGATTTTG AAGAAAAGCA AATTCAGAGA CCGCTCCGTC 
TATGTTCCCA AGGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCACGAAAG GATAGCTCTG GGCAATGAAG AAGGATTATT TGTTGTGCAC 
ATCACCAAAG ATGAAATTAT TAGAGTTGGT GATAATAAGA AGATACATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATATCAG CTTTGGACGG ACGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGG 
TGTCAGACCA TAACTTCGGG CAAAGTGTGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGGAAATTT 
AAGGAAATTC AAGTCCCATA TAACGTGCAA TGGATGGCGA TCTTCAGTGA ACAGCTCTGC 
GTGGGATTCC AGTCGGGATT CCTAAGATAC CCGTTGAACG GAGAGGGAAG CCCATACAGT 
ATGCTCCATT CAAATGACCA CACACTGTCA TTTATTGCCC ACCAGCCAAT GGATGCCATC 
TGCGCAGTTG AGATCTCCAG TAAAGAGTAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGGAG ATCGAGACAA CAGGAGTTGA TGTGGCCGTC AAATCCCTCC 
TCTTGTTGTT ACAACGCACC ATATCTCTCA GTGTATAGTG AGAATGCAGT TGATATCTTC 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG ACCCTTAAAC 
AGTGAAGGAT CATTAAACCT TTTAGGGCTG GAGACAATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTGGTGGTT CCTGAAACAT CGGACAATAG TCGGAAGCAG 
ATGGTTAGAA ACATCAACAA CAAGCGACGC TTCTCCTTCA GGGTCCCAGA AGAGGAGAGG 
ATGCAGCAGA GGAGAGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGCCCAGGGG ATGGAATACA GATCCTAAAA 
GACCTGCCTA TGCCAGGCTT CCCTTATCCA TCCCCAAATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAGCA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGCATATTCC CCGTCTCTGC GCTCTGTCCC CGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGCC GGACCGTGTT CAGCGGCTCA 
GTCAGTATTC CATCCATCAC TAAATCCCGC CCTGAGCCCG GCCGCTCCAT GAGCGCCAGC 
AGCGGCCTTT CGGCAAGGTC ATCTGCACAG AATGGCAGTG CATTAAAGAG AGAATTCTCC 
GGAGGAAGCT ATAATGCTAA GTGGCAGCCC ATGCCCTCCC CATCAGAGGG CTCCTTGTCT 
TCCGGAGGCA TGGACCAGGG AAGTGACGCC CCAGCCAGGG ACTATGACGG AGAGGACTCG 
GATTCTCCGA GGCACTCCAC AGCTTCCAAC AGCTCCAACC TCAGCAGCCC GCCCAGCCCA 
GTGTCACCTC GGAAGACCAA GAGCCTGTCC CTGGAGAGCA CCGACCGTGG GACCTGGGAC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_005622627.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622684.1 Canis lupus familiaris CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X13, mRNA.

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CloneID	OCe09391
Clone ID Related Accession (Same CDS sequence)	XM_005622627.1 , XM_005622627.2
Accession Version	XM_005622627.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase MRCK alpha isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCGTCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTATTGTAG ACAGTACTCC TCTTCCAGCT CACACACCAA CCTTAAGGAA AAAGGGATGT 
CCTGGTTCAA CTGGCTTTCC AGCTAAGCGC AAGACTCATC AGTTTTTTGT CAAATCTTTC 
ACAACACCTA CCAGATGTCA CCAGTGCACC TCTCTGATGG TTGGTTTGAT AAGACAAGGC 
TGTTCCTGTG AGGTGTGCGG ATTCTCATGT CATATAACTT GTGTAAACAA AGCTCCAACT 
GCTTGTCCAG TCCCTCCTGA GCAGACAAAA GGTCCTCTGG GGATAGATCC ACAGAAAGGA 
ATAGGAACAG CCTATGAAGG TCATGTCAGG ATTCCGAAAC CAGCTGGAGT AAAGAAAGGA 
TGGCAGAGAG CCCTGGCTGT GGTTTGTGAC TTTAAACTCT TCCTGTATGA TGTCGCTGAA 
GGAAAAGCAT CTCAGCCCAG CGTTGTGGTC AGTCAGGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTGTT GGCTTCTGAT GTTATTCATG CAAGTCGAAA AGATATACCC 
TGTATATTCA GAGTCACAGC TTCCCAGCTC TCAGCATCTG ACAGCAGGTG TTCGGTCCTA 
ATGCTCGCAG AGAGTGAGAG CGAGAGGAAC AAGTGGGTGG GCGTGCTCAA CGAGCTGCAT 
AAGATTTTGA AGAAAAGCAA ATTCAGAGAC CGCTCCGTCT ATGTTCCCAA GGAGGCTTAT 
GACAGCACTC TGCCCCTCAT TAAAACAACC CAGGCAGCTG CAATCATAGA TCACGAAAGG 
ATAGCTCTGG GCAATGAAGA AGGATTATTT GTTGTGCACA TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ATAATAAGAA GATACATCAG ATTGAACTCA TTCCAAATGA TCAGCTTGTT 
GCTGTGATCT CAGGACGAAA TCGTCATGTA CGACTTTTTC CTATATCAGC TTTGGACGGA 
CGAGAGACTG ATTTTTATAA GCTGGCAGAA ACTAAAGGGT GTCAGACCAT AACTTCGGGC 
AAAGTGTGCC ATGGAGCTCT CACATGCCTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACCCGTCAC AGGAAATTTA AGGAAATTCA AGTCCCATAT 
AACGTGCAAT GGATGGCGAT CTTCAGTGAA CAGCTCTGCG TGGGATTCCA GTCGGGATTC 
CTAAGATACC CGTTGAACGG AGAGGGAAGC CCATACAGTA TGCTCCATTC AAATGACCAC 
ACACTGTCAT TTATTGCCCA CCAGCCAATG GATGCCATCT GCGCAGTTGA GATCTCCAGT 
AAAGAGTATC TGCTGTGTTT TAACAGCATT GGGATATACA CTGACTGCCA GGGCCGGAGA 
TCGAGACAAC AGGAGTTGAT GTGGCCGTCA AATCCCTCCT CTTGTTGTTA CAACGCACCA 
TATCTCTCAG TGTATAGTGA GAATGCAGTT GATATCTTCG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TCCCACTCAA AAAGGTTCGA CCCTTAAACA GTGAAGGATC ATTAAACCTT 
TTAGGGCTGG AGACAATTAG ATTAATATAT TTCAAAAATA AGATGGCAGA AGGGGATGAA 
CTGGTGGTTC CTGAAACATC GGACAATAGT CGGAAGCAGA TGGTTAGAAA CATCAACAAC 
AAGCGACGCT TCTCCTTCAG GGTCCCAGAA GAGGAGAGGA TGCAGCAGAG GAGAGAGATG 
CTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAACC CAACTAATTT TAACCACATA 
GCACACATGG GCCCAGGGGA TGGAATACAG ATCCTAAAAG ACCTGCCTAT GAACCCTCGG 
CCTCAGGAAA GCCGGACCGT GTTCAGCGGC TCAGTCAGTA TTCCATCCAT CACTAAATCC 
CGCCCTGAGC CCGGCCGCTC CATGAGCGCC AGCAGCGGCC TTTCGGCAAG GTCATCTGCA 
CAGAATGGCA GTGCATTAAA GAGAGAATTC TCCGGAGGAA GCTATAATGC TAAGTGGCAG 
CCCATGCCCT CCCCATCAGA GGGCTCCTTG TCTTCCGGAG GCATGGACCA GGGAAGTGAC 
GCCCCAGCCA GGGACTATGA CGGAGAGGAC TCGGATTCTC CGAGGCACTC CACAGCTTCC 
AACAGCTCCA ACCTCAGCAG CCCGCCCAGC CCAGTGTCAC CTCGGAAGAC CAAGAGCCTG 
TCCCTGGAGA GCACCGACCG TGGGACCTGG GACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622684.1
CDS	384..5582
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPASLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKTLKEEIENLRKQIRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIRTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGLCSIEHQQEISKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLYENVSMRNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGARATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRIVDSTPLPAHTPTLRKKGCPGSTGFPAKRKTHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDVAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASDSRCSVLMLAESESERNKWVGVLNELHKILKKSKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHITKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPISALDGRETDFYKLAETKGCQTITSGKVCHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPSNPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRFSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNAKWQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGTWDP

Target ORF information:

RefSeq Version	XM_005622627.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622627.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCGTCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTATTGTAG ACAGTACTCC TCTTCCAGCT CACACACCAA CCTTAAGGAA AAAGGGATGT 
CCTGGTTCAA CTGGCTTTCC AGCTAAGCGC AAGACTCATC AGTTTTTTGT CAAATCTTTC 
ACAACACCTA CCAGATGTCA CCAGTGCACC TCTCTGATGG TTGGTTTGAT AAGACAAGGC 
TGTTCCTGTG AGGTGTGCGG ATTCTCATGT CATATAACTT GTGTAAACAA AGCTCCAACT 
GCTTGTCCAG TCCCTCCTGA GCAGACAAAA GGTCCTCTGG GGATAGATCC ACAGAAAGGA 
ATAGGAACAG CCTATGAAGG TCATGTCAGG ATTCCGAAAC CAGCTGGAGT AAAGAAAGGA 
TGGCAGAGAG CCCTGGCTGT GGTTTGTGAC TTTAAACTCT TCCTGTATGA TGTCGCTGAA 
GGAAAAGCAT CTCAGCCCAG CGTTGTGGTC AGTCAGGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTGTT GGCTTCTGAT GTTATTCATG CAAGTCGAAA AGATATACCC 
TGTATATTCA GAGTCACAGC TTCCCAGCTC TCAGCATCTG ACAGCAGGTG TTCGGTCCTA 
ATGCTCGCAG AGAGTGAGAG CGAGAGGAAC AAGTGGGTGG GCGTGCTCAA CGAGCTGCAT 
AAGATTTTGA AGAAAAGCAA ATTCAGAGAC CGCTCCGTCT ATGTTCCCAA GGAGGCTTAT 
GACAGCACTC TGCCCCTCAT TAAAACAACC CAGGCAGCTG CAATCATAGA TCACGAAAGG 
ATAGCTCTGG GCAATGAAGA AGGATTATTT GTTGTGCACA TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ATAATAAGAA GATACATCAG ATTGAACTCA TTCCAAATGA TCAGCTTGTT 
GCTGTGATCT CAGGACGAAA TCGTCATGTA CGACTTTTTC CTATATCAGC TTTGGACGGA 
CGAGAGACTG ATTTTTATAA GCTGGCAGAA ACTAAAGGGT GTCAGACCAT AACTTCGGGC 
AAAGTGTGCC ATGGAGCTCT CACATGCCTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACCCGTCAC AGGAAATTTA AGGAAATTCA AGTCCCATAT 
AACGTGCAAT GGATGGCGAT CTTCAGTGAA CAGCTCTGCG TGGGATTCCA GTCGGGATTC 
CTAAGATACC CGTTGAACGG AGAGGGAAGC CCATACAGTA TGCTCCATTC AAATGACCAC 
ACACTGTCAT TTATTGCCCA CCAGCCAATG GATGCCATCT GCGCAGTTGA GATCTCCAGT 
AAAGAGTATC TGCTGTGTTT TAACAGCATT GGGATATACA CTGACTGCCA GGGCCGGAGA 
TCGAGACAAC AGGAGTTGAT GTGGCCGTCA AATCCCTCCT CTTGTTGTTA CAACGCACCA 
TATCTCTCAG TGTATAGTGA GAATGCAGTT GATATCTTCG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TCCCACTCAA AAAGGTTCGA CCCTTAAACA GTGAAGGATC ATTAAACCTT 
TTAGGGCTGG AGACAATTAG ATTAATATAT TTCAAAAATA AGATGGCAGA AGGGGATGAA 
CTGGTGGTTC CTGAAACATC GGACAATAGT CGGAAGCAGA TGGTTAGAAA CATCAACAAC 
AAGCGACGCT TCTCCTTCAG GGTCCCAGAA GAGGAGAGGA TGCAGCAGAG GAGAGAGATG 
CTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAACC CAACTAATTT TAACCACATA 
GCACACATGG GCCCAGGGGA TGGAATACAG ATCCTAAAAG ACCTGCCTAT GAACCCTCGG 
CCTCAGGAAA GCCGGACCGT GTTCAGCGGC TCAGTCAGTA TTCCATCCAT CACTAAATCC 
CGCCCTGAGC CCGGCCGCTC CATGAGCGCC AGCAGCGGCC TTTCGGCAAG GTCATCTGCA 
CAGAATGGCA GTGCATTAAA GAGAGAATTC TCCGGAGGAA GCTATAATGC TAAGTGGCAG 
CCCATGCCCT CCCCATCAGA GGGCTCCTTG TCTTCCGGAG GCATGGACCA GGGAAGTGAC 
GCCCCAGCCA GGGACTATGA CGGAGAGGAC TCGGATTCTC CGAGGCACTC CACAGCTTCC 
AACAGCTCCA ACCTCAGCAG CCCGCCCAGC CCAGTGTCAC CTCGGAAGAC CAAGAGCCTG 
TCCCTGGAGA GCACCGACCG TGGGACCTGG GACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_005622627.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622684.1 Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X17, mRNA.

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CloneID	OCe09391
Clone ID Related Accession (Same CDS sequence)	XM_005622627.1 , XM_005622627.2
Accession Version	XM_005622627.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase MRCK alpha isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005622627.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCGTCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTATTGTAG ACAGTACTCC TCTTCCAGCT CACACACCAA CCTTAAGGAA AAAGGGATGT 
CCTGGTTCAA CTGGCTTTCC AGCTAAGCGC AAGACTCATC AGTTTTTTGT CAAATCTTTC 
ACAACACCTA CCAGATGTCA CCAGTGCACC TCTCTGATGG TTGGTTTGAT AAGACAAGGC 
TGTTCCTGTG AGGTGTGCGG ATTCTCATGT CATATAACTT GTGTAAACAA AGCTCCAACT 
GCTTGTCCAG TCCCTCCTGA GCAGACAAAA GGTCCTCTGG GGATAGATCC ACAGAAAGGA 
ATAGGAACAG CCTATGAAGG TCATGTCAGG ATTCCGAAAC CAGCTGGAGT AAAGAAAGGA 
TGGCAGAGAG CCCTGGCTGT GGTTTGTGAC TTTAAACTCT TCCTGTATGA TGTCGCTGAA 
GGAAAAGCAT CTCAGCCCAG CGTTGTGGTC AGTCAGGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTGTT GGCTTCTGAT GTTATTCATG CAAGTCGAAA AGATATACCC 
TGTATATTCA GAGTCACAGC TTCCCAGCTC TCAGCATCTG ACAGCAGGTG TTCGGTCCTA 
ATGCTCGCAG AGAGTGAGAG CGAGAGGAAC AAGTGGGTGG GCGTGCTCAA CGAGCTGCAT 
AAGATTTTGA AGAAAAGCAA ATTCAGAGAC CGCTCCGTCT ATGTTCCCAA GGAGGCTTAT 
GACAGCACTC TGCCCCTCAT TAAAACAACC CAGGCAGCTG CAATCATAGA TCACGAAAGG 
ATAGCTCTGG GCAATGAAGA AGGATTATTT GTTGTGCACA TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ATAATAAGAA GATACATCAG ATTGAACTCA TTCCAAATGA TCAGCTTGTT 
GCTGTGATCT CAGGACGAAA TCGTCATGTA CGACTTTTTC CTATATCAGC TTTGGACGGA 
CGAGAGACTG ATTTTTATAA GCTGGCAGAA ACTAAAGGGT GTCAGACCAT AACTTCGGGC 
AAAGTGTGCC ATGGAGCTCT CACATGCCTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACCCGTCAC AGGAAATTTA AGGAAATTCA AGTCCCATAT 
AACGTGCAAT GGATGGCGAT CTTCAGTGAA CAGCTCTGCG TGGGATTCCA GTCGGGATTC 
CTAAGATACC CGTTGAACGG AGAGGGAAGC CCATACAGTA TGCTCCATTC AAATGACCAC 
ACACTGTCAT TTATTGCCCA CCAGCCAATG GATGCCATCT GCGCAGTTGA GATCTCCAGT 
AAAGAGTATC TGCTGTGTTT TAACAGCATT GGGATATACA CTGACTGCCA GGGCCGGAGA 
TCGAGACAAC AGGAGTTGAT GTGGCCGTCA AATCCCTCCT CTTGTTGTTA CAACGCACCA 
TATCTCTCAG TGTATAGTGA GAATGCAGTT GATATCTTCG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TCCCACTCAA AAAGGTTCGA CCCTTAAACA GTGAAGGATC ATTAAACCTT 
TTAGGGCTGG AGACAATTAG ATTAATATAT TTCAAAAATA AGATGGCAGA AGGGGATGAA 
CTGGTGGTTC CTGAAACATC GGACAATAGT CGGAAGCAGA TGGTTAGAAA CATCAACAAC 
AAGCGACGCT TCTCCTTCAG GGTCCCAGAA GAGGAGAGGA TGCAGCAGAG GAGAGAGATG 
CTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAACC CAACTAATTT TAACCACATA 
GCACACATGG GCCCAGGGGA TGGAATACAG ATCCTAAAAG ACCTGCCTAT GAACCCTCGG 
CCTCAGGAAA GCCGGACCGT GTTCAGCGGC TCAGTCAGTA TTCCATCCAT CACTAAATCC 
CGCCCTGAGC CCGGCCGCTC CATGAGCGCC AGCAGCGGCC TTTCGGCAAG GTCATCTGCA 
CAGAATGGCA GTGCATTAAA GAGAGAATTC TCCGGAGGAA GCTATAATGC TAAGTGGCAG 
CCCATGCCCT CCCCATCAGA GGGCTCCTTG TCTTCCGGAG GCATGGACCA GGGAAGTGAC 
GCCCCAGCCA GGGACTATGA CGGAGAGGAC TCGGATTCTC CGAGGCACTC CACAGCTTCC 
AACAGCTCCA ACCTCAGCAG CCCGCCCAGC CCAGTGTCAC CTCGGAAGAC CAAGAGCCTG 
TCCCTGGAGA GCACCGACCG TGGGACCTGG GACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622684.1
CDS	1..5199
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPASLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKTLKEEIENLRKQIRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIRTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGLCSIEHQQEISKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLYENVSMRNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGARATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRIVDSTPLPAHTPTLRKKGCPGSTGFPAKRKTHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDVAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASDSRCSVLMLAESESERNKWVGVLNELHKILKKSKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHITKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPISALDGRETDFYKLAETKGCQTITSGKVCHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPSNPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRFSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNAKWQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGTWDP

Target ORF information:

RefSeq Version	XM_005622627.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622627.2

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCGTCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTATTGTAG ACAGTACTCC TCTTCCAGCT CACACACCAA CCTTAAGGAA AAAGGGATGT 
CCTGGTTCAA CTGGCTTTCC AGCTAAGCGC AAGACTCATC AGTTTTTTGT CAAATCTTTC 
ACAACACCTA CCAGATGTCA CCAGTGCACC TCTCTGATGG TTGGTTTGAT AAGACAAGGC 
TGTTCCTGTG AGGTGTGCGG ATTCTCATGT CATATAACTT GTGTAAACAA AGCTCCAACT 
GCTTGTCCAG TCCCTCCTGA GCAGACAAAA GGTCCTCTGG GGATAGATCC ACAGAAAGGA 
ATAGGAACAG CCTATGAAGG TCATGTCAGG ATTCCGAAAC CAGCTGGAGT AAAGAAAGGA 
TGGCAGAGAG CCCTGGCTGT GGTTTGTGAC TTTAAACTCT TCCTGTATGA TGTCGCTGAA 
GGAAAAGCAT CTCAGCCCAG CGTTGTGGTC AGTCAGGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTGTT GGCTTCTGAT GTTATTCATG CAAGTCGAAA AGATATACCC 
TGTATATTCA GAGTCACAGC TTCCCAGCTC TCAGCATCTG ACAGCAGGTG TTCGGTCCTA 
ATGCTCGCAG AGAGTGAGAG CGAGAGGAAC AAGTGGGTGG GCGTGCTCAA CGAGCTGCAT 
AAGATTTTGA AGAAAAGCAA ATTCAGAGAC CGCTCCGTCT ATGTTCCCAA GGAGGCTTAT 
GACAGCACTC TGCCCCTCAT TAAAACAACC CAGGCAGCTG CAATCATAGA TCACGAAAGG 
ATAGCTCTGG GCAATGAAGA AGGATTATTT GTTGTGCACA TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ATAATAAGAA GATACATCAG ATTGAACTCA TTCCAAATGA TCAGCTTGTT 
GCTGTGATCT CAGGACGAAA TCGTCATGTA CGACTTTTTC CTATATCAGC TTTGGACGGA 
CGAGAGACTG ATTTTTATAA GCTGGCAGAA ACTAAAGGGT GTCAGACCAT AACTTCGGGC 
AAAGTGTGCC ATGGAGCTCT CACATGCCTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACCCGTCAC AGGAAATTTA AGGAAATTCA AGTCCCATAT 
AACGTGCAAT GGATGGCGAT CTTCAGTGAA CAGCTCTGCG TGGGATTCCA GTCGGGATTC 
CTAAGATACC CGTTGAACGG AGAGGGAAGC CCATACAGTA TGCTCCATTC AAATGACCAC 
ACACTGTCAT TTATTGCCCA CCAGCCAATG GATGCCATCT GCGCAGTTGA GATCTCCAGT 
AAAGAGTATC TGCTGTGTTT TAACAGCATT GGGATATACA CTGACTGCCA GGGCCGGAGA 
TCGAGACAAC AGGAGTTGAT GTGGCCGTCA AATCCCTCCT CTTGTTGTTA CAACGCACCA 
TATCTCTCAG TGTATAGTGA GAATGCAGTT GATATCTTCG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TCCCACTCAA AAAGGTTCGA CCCTTAAACA GTGAAGGATC ATTAAACCTT 
TTAGGGCTGG AGACAATTAG ATTAATATAT TTCAAAAATA AGATGGCAGA AGGGGATGAA 
CTGGTGGTTC CTGAAACATC GGACAATAGT CGGAAGCAGA TGGTTAGAAA CATCAACAAC 
AAGCGACGCT TCTCCTTCAG GGTCCCAGAA GAGGAGAGGA TGCAGCAGAG GAGAGAGATG 
CTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAACC CAACTAATTT TAACCACATA 
GCACACATGG GCCCAGGGGA TGGAATACAG ATCCTAAAAG ACCTGCCTAT GAACCCTCGG 
CCTCAGGAAA GCCGGACCGT GTTCAGCGGC TCAGTCAGTA TTCCATCCAT CACTAAATCC 
CGCCCTGAGC CCGGCCGCTC CATGAGCGCC AGCAGCGGCC TTTCGGCAAG GTCATCTGCA 
CAGAATGGCA GTGCATTAAA GAGAGAATTC TCCGGAGGAA GCTATAATGC TAAGTGGCAG 
CCCATGCCCT CCCCATCAGA GGGCTCCTTG TCTTCCGGAG GCATGGACCA GGGAAGTGAC 
GCCCCAGCCA GGGACTATGA CGGAGAGGAC TCGGATTCTC CGAGGCACTC CACAGCTTCC 
AACAGCTCCA ACCTCAGCAG CCCGCCCAGC CCAGTGTCAC CTCGGAAGAC CAAGAGCCTG 
TCCCTGGAGA GCACCGACCG TGGGACCTGG GACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_858573.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_863666.1 Canis lupus familiaris CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X15, mRNA.

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CloneID	OCe09392
Clone ID Related Accession (Same CDS sequence)	XM_858573.2 , XM_858573.3
Accession Version	XM_858573.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase MRCK alpha isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_858573.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAATTGTA GACAGTACTC CTCTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG TCCTGGTTCA ACTGGCTTTC CAGCTAAGCG CAAGACTCAT 
CAGTTTTTTG TCAAATCTTT CACAACACCT ACCAGATGTC ACCAGTGCAC CTCTCTGATG 
GTTGGTTTGA TAAGACAAGG CTGTTCCTGT GAGGTGTGCG GATTCTCATG TCATATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTCCCTCCTG AGCAGACAAA AGGTCCTCTG 
GGGATAGATC CACAGAAAGG AATAGGAACA GCCTATGAAG GTCATGTCAG GATTCCGAAA 
CCAGCTGGAG TAAAGAAAGG ATGGCAGAGA GCCCTGGCTG TGGTTTGTGA CTTTAAACTC 
TTCCTGTATG ATGTCGCTGA AGGAAAAGCA TCTCAGCCCA GCGTTGTGGT CAGTCAGGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTGT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTC AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
GACAGCAGGT GTTCGGTCCT AATGCTCGCA GAGAGTGAGA GCGAGAGGAA CAAGTGGGTG 
GGCGTGCTCA ACGAGCTGCA TAAGATTTTG AAGAAAAGCA AATTCAGAGA CCGCTCCGTC 
TATGTTCCCA AGGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCACGAAAG GATAGCTCTG GGCAATGAAG AAGGATTATT TGTTGTGCAC 
ATCACCAAAG ATGAAATTAT TAGAGTTGGT GATAATAAGA AGATACATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATATCAG CTTTGGACGG ACGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGG 
TGTCAGACCA TAACTTCGGG CAAAGTGTGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGGAAATTT 
AAGGAAATTC AAGTCCCATA TAACGTGCAA TGGATGGCGA TCTTCAGTGA ACAGCTCTGC 
GTGGGATTCC AGTCGGGATT CCTAAGATAC CCGTTGAACG GAGAGGGAAG CCCATACAGT 
ATGCTCCATT CAAATGACCA CACACTGTCA TTTATTGCCC ACCAGCCAAT GGATGCCATC 
TGCGCAGTTG AGATCTCCAG TAAAGAGTAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGGAG ATCGAGACAA CAGGAGTTGA TGTGGCCGTC AAATCCCTCC 
TCTTGTTGTT ACAACGCACC ATATCTCTCA GTGTATAGTG AGAATGCAGT TGATATCTTC 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG ACCCTTAAAC 
AGTGAAGGAT CATTAAACCT TTTAGGGCTG GAGACAATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTGGTGGTT CCTGAAACAT CGGACAATAG TCGGAAGCAG 
ATGGTTAGAA ACATCAACAA CAAGCGACGC TTCTCCTTCA GGGTCCCAGA AGAGGAGAGG 
ATGCAGCAGA GGAGAGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGCCCAGGGG ATGGAATACA GATCCTAAAA 
GACCTGCCTA TGAACCCTCG GCCTCAGGAA AGCCGGACCG TGTTCAGCGG CTCAGTCAGT 
ATTCCATCCA TCACTAAATC CCGCCCTGAG CCCGGCCGCT CCATGAGCGC CAGCAGCGGC 
CTTTCGGCAA GGTCATCTGC ACAGAATGGC AGTGCATTAA AGAGAGAATT CTCCGGAGGA 
AGCTATAATG CTAAGTGGCA GCCCATGCCC TCCCCATCAG AGGGCTCCTT GTCTTCCGGA 
GGCATGGACC AGGGAAGTGA CGCCCCAGCC AGGGACTATG ACGGAGAGGA CTCGGATTCT 
CCGAGGCACT CCACAGCTTC CAACAGCTCC AACCTCAGCA GCCCGCCCAG CCCAGTGTCA 
CCTCGGAAGA CCAAGAGCCT GTCCCTGGAG AGCACCGACC GTGGGACCTG GGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_863666.1
CDS	384..5543
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPASLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKTLKEEIENLRKQIRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIRTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGLCSIEHQQEISKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLYENVSMRNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGARATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEIVDSTPLPAHTPTLRKKGCPGSTGFPAKRKTHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDVAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASDSRCSVLMLAESESERNKWVGVLNELHKILKKSKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHITKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPISALDGRETDFYKLAETKGCQTITSGKVCHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPSNPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRFSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNAKWQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGTWDP

Target ORF information:

RefSeq Version	XM_858573.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_858573.2

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAATTGTA GACAGTACTC CTCTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG TCCTGGTTCA ACTGGCTTTC CAGCTAAGCG CAAGACTCAT 
CAGTTTTTTG TCAAATCTTT CACAACACCT ACCAGATGTC ACCAGTGCAC CTCTCTGATG 
GTTGGTTTGA TAAGACAAGG CTGTTCCTGT GAGGTGTGCG GATTCTCATG TCATATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTCCCTCCTG AGCAGACAAA AGGTCCTCTG 
GGGATAGATC CACAGAAAGG AATAGGAACA GCCTATGAAG GTCATGTCAG GATTCCGAAA 
CCAGCTGGAG TAAAGAAAGG ATGGCAGAGA GCCCTGGCTG TGGTTTGTGA CTTTAAACTC 
TTCCTGTATG ATGTCGCTGA AGGAAAAGCA TCTCAGCCCA GCGTTGTGGT CAGTCAGGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTGT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTC AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
GACAGCAGGT GTTCGGTCCT AATGCTCGCA GAGAGTGAGA GCGAGAGGAA CAAGTGGGTG 
GGCGTGCTCA ACGAGCTGCA TAAGATTTTG AAGAAAAGCA AATTCAGAGA CCGCTCCGTC 
TATGTTCCCA AGGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCACGAAAG GATAGCTCTG GGCAATGAAG AAGGATTATT TGTTGTGCAC 
ATCACCAAAG ATGAAATTAT TAGAGTTGGT GATAATAAGA AGATACATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATATCAG CTTTGGACGG ACGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGG 
TGTCAGACCA TAACTTCGGG CAAAGTGTGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGGAAATTT 
AAGGAAATTC AAGTCCCATA TAACGTGCAA TGGATGGCGA TCTTCAGTGA ACAGCTCTGC 
GTGGGATTCC AGTCGGGATT CCTAAGATAC CCGTTGAACG GAGAGGGAAG CCCATACAGT 
ATGCTCCATT CAAATGACCA CACACTGTCA TTTATTGCCC ACCAGCCAAT GGATGCCATC 
TGCGCAGTTG AGATCTCCAG TAAAGAGTAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGGAG ATCGAGACAA CAGGAGTTGA TGTGGCCGTC AAATCCCTCC 
TCTTGTTGTT ACAACGCACC ATATCTCTCA GTGTATAGTG AGAATGCAGT TGATATCTTC 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG ACCCTTAAAC 
AGTGAAGGAT CATTAAACCT TTTAGGGCTG GAGACAATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTGGTGGTT CCTGAAACAT CGGACAATAG TCGGAAGCAG 
ATGGTTAGAA ACATCAACAA CAAGCGACGC TTCTCCTTCA GGGTCCCAGA AGAGGAGAGG 
ATGCAGCAGA GGAGAGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGCCCAGGGG ATGGAATACA GATCCTAAAA 
GACCTGCCTA TGAACCCTCG GCCTCAGGAA AGCCGGACCG TGTTCAGCGG CTCAGTCAGT 
ATTCCATCCA TCACTAAATC CCGCCCTGAG CCCGGCCGCT CCATGAGCGC CAGCAGCGGC 
CTTTCGGCAA GGTCATCTGC ACAGAATGGC AGTGCATTAA AGAGAGAATT CTCCGGAGGA 
AGCTATAATG CTAAGTGGCA GCCCATGCCC TCCCCATCAG AGGGCTCCTT GTCTTCCGGA 
GGCATGGACC AGGGAAGTGA CGCCCCAGCC AGGGACTATG ACGGAGAGGA CTCGGATTCT 
CCGAGGCACT CCACAGCTTC CAACAGCTCC AACCTCAGCA GCCCGCCCAG CCCAGTGTCA 
CCTCGGAAGA CCAAGAGCCT GTCCCTGGAG AGCACCGACC GTGGGACCTG GGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_858573.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_863666.1 Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X19, mRNA.

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CloneID	OCe09392
Clone ID Related Accession (Same CDS sequence)	XM_858573.2 , XM_858573.3
Accession Version	XM_858573.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase MRCK alpha isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_858573.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAATTGTA GACAGTACTC CTCTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG TCCTGGTTCA ACTGGCTTTC CAGCTAAGCG CAAGACTCAT 
CAGTTTTTTG TCAAATCTTT CACAACACCT ACCAGATGTC ACCAGTGCAC CTCTCTGATG 
GTTGGTTTGA TAAGACAAGG CTGTTCCTGT GAGGTGTGCG GATTCTCATG TCATATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTCCCTCCTG AGCAGACAAA AGGTCCTCTG 
GGGATAGATC CACAGAAAGG AATAGGAACA GCCTATGAAG GTCATGTCAG GATTCCGAAA 
CCAGCTGGAG TAAAGAAAGG ATGGCAGAGA GCCCTGGCTG TGGTTTGTGA CTTTAAACTC 
TTCCTGTATG ATGTCGCTGA AGGAAAAGCA TCTCAGCCCA GCGTTGTGGT CAGTCAGGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTGT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTC AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
GACAGCAGGT GTTCGGTCCT AATGCTCGCA GAGAGTGAGA GCGAGAGGAA CAAGTGGGTG 
GGCGTGCTCA ACGAGCTGCA TAAGATTTTG AAGAAAAGCA AATTCAGAGA CCGCTCCGTC 
TATGTTCCCA AGGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCACGAAAG GATAGCTCTG GGCAATGAAG AAGGATTATT TGTTGTGCAC 
ATCACCAAAG ATGAAATTAT TAGAGTTGGT GATAATAAGA AGATACATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATATCAG CTTTGGACGG ACGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGG 
TGTCAGACCA TAACTTCGGG CAAAGTGTGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGGAAATTT 
AAGGAAATTC AAGTCCCATA TAACGTGCAA TGGATGGCGA TCTTCAGTGA ACAGCTCTGC 
GTGGGATTCC AGTCGGGATT CCTAAGATAC CCGTTGAACG GAGAGGGAAG CCCATACAGT 
ATGCTCCATT CAAATGACCA CACACTGTCA TTTATTGCCC ACCAGCCAAT GGATGCCATC 
TGCGCAGTTG AGATCTCCAG TAAAGAGTAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGGAG ATCGAGACAA CAGGAGTTGA TGTGGCCGTC AAATCCCTCC 
TCTTGTTGTT ACAACGCACC ATATCTCTCA GTGTATAGTG AGAATGCAGT TGATATCTTC 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG ACCCTTAAAC 
AGTGAAGGAT CATTAAACCT TTTAGGGCTG GAGACAATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTGGTGGTT CCTGAAACAT CGGACAATAG TCGGAAGCAG 
ATGGTTAGAA ACATCAACAA CAAGCGACGC TTCTCCTTCA GGGTCCCAGA AGAGGAGAGG 
ATGCAGCAGA GGAGAGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGCCCAGGGG ATGGAATACA GATCCTAAAA 
GACCTGCCTA TGAACCCTCG GCCTCAGGAA AGCCGGACCG TGTTCAGCGG CTCAGTCAGT 
ATTCCATCCA TCACTAAATC CCGCCCTGAG CCCGGCCGCT CCATGAGCGC CAGCAGCGGC 
CTTTCGGCAA GGTCATCTGC ACAGAATGGC AGTGCATTAA AGAGAGAATT CTCCGGAGGA 
AGCTATAATG CTAAGTGGCA GCCCATGCCC TCCCCATCAG AGGGCTCCTT GTCTTCCGGA 
GGCATGGACC AGGGAAGTGA CGCCCCAGCC AGGGACTATG ACGGAGAGGA CTCGGATTCT 
CCGAGGCACT CCACAGCTTC CAACAGCTCC AACCTCAGCA GCCCGCCCAG CCCAGTGTCA 
CCTCGGAAGA CCAAGAGCCT GTCCCTGGAG AGCACCGACC GTGGGACCTG GGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_863666.1
CDS	1..5160
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPASLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKTLKEEIENLRKQIRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIRTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGLCSIEHQQEISKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLYENVSMRNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGARATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEIVDSTPLPAHTPTLRKKGCPGSTGFPAKRKTHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDVAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASDSRCSVLMLAESESERNKWVGVLNELHKILKKSKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHITKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPISALDGRETDFYKLAETKGCQTITSGKVCHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPSNPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRFSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNAKWQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGTWDP

Target ORF information:

RefSeq Version	XM_858573.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_858573.3

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAATTGTA GACAGTACTC CTCTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG TCCTGGTTCA ACTGGCTTTC CAGCTAAGCG CAAGACTCAT 
CAGTTTTTTG TCAAATCTTT CACAACACCT ACCAGATGTC ACCAGTGCAC CTCTCTGATG 
GTTGGTTTGA TAAGACAAGG CTGTTCCTGT GAGGTGTGCG GATTCTCATG TCATATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTCCCTCCTG AGCAGACAAA AGGTCCTCTG 
GGGATAGATC CACAGAAAGG AATAGGAACA GCCTATGAAG GTCATGTCAG GATTCCGAAA 
CCAGCTGGAG TAAAGAAAGG ATGGCAGAGA GCCCTGGCTG TGGTTTGTGA CTTTAAACTC 
TTCCTGTATG ATGTCGCTGA AGGAAAAGCA TCTCAGCCCA GCGTTGTGGT CAGTCAGGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTGT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTC AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
GACAGCAGGT GTTCGGTCCT AATGCTCGCA GAGAGTGAGA GCGAGAGGAA CAAGTGGGTG 
GGCGTGCTCA ACGAGCTGCA TAAGATTTTG AAGAAAAGCA AATTCAGAGA CCGCTCCGTC 
TATGTTCCCA AGGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCACGAAAG GATAGCTCTG GGCAATGAAG AAGGATTATT TGTTGTGCAC 
ATCACCAAAG ATGAAATTAT TAGAGTTGGT GATAATAAGA AGATACATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATATCAG CTTTGGACGG ACGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGG 
TGTCAGACCA TAACTTCGGG CAAAGTGTGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGGAAATTT 
AAGGAAATTC AAGTCCCATA TAACGTGCAA TGGATGGCGA TCTTCAGTGA ACAGCTCTGC 
GTGGGATTCC AGTCGGGATT CCTAAGATAC CCGTTGAACG GAGAGGGAAG CCCATACAGT 
ATGCTCCATT CAAATGACCA CACACTGTCA TTTATTGCCC ACCAGCCAAT GGATGCCATC 
TGCGCAGTTG AGATCTCCAG TAAAGAGTAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGGAG ATCGAGACAA CAGGAGTTGA TGTGGCCGTC AAATCCCTCC 
TCTTGTTGTT ACAACGCACC ATATCTCTCA GTGTATAGTG AGAATGCAGT TGATATCTTC 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG ACCCTTAAAC 
AGTGAAGGAT CATTAAACCT TTTAGGGCTG GAGACAATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTGGTGGTT CCTGAAACAT CGGACAATAG TCGGAAGCAG 
ATGGTTAGAA ACATCAACAA CAAGCGACGC TTCTCCTTCA GGGTCCCAGA AGAGGAGAGG 
ATGCAGCAGA GGAGAGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGCCCAGGGG ATGGAATACA GATCCTAAAA 
GACCTGCCTA TGAACCCTCG GCCTCAGGAA AGCCGGACCG TGTTCAGCGG CTCAGTCAGT 
ATTCCATCCA TCACTAAATC CCGCCCTGAG CCCGGCCGCT CCATGAGCGC CAGCAGCGGC 
CTTTCGGCAA GGTCATCTGC ACAGAATGGC AGTGCATTAA AGAGAGAATT CTCCGGAGGA 
AGCTATAATG CTAAGTGGCA GCCCATGCCC TCCCCATCAG AGGGCTCCTT GTCTTCCGGA 
GGCATGGACC AGGGAAGTGA CGCCCCAGCC AGGGACTATG ACGGAGAGGA CTCGGATTCT 
CCGAGGCACT CCACAGCTTC CAACAGCTCC AACCTCAGCA GCCCGCCCAG CCCAGTGTCA 
CCTCGGAAGA CCAAGAGCCT GTCCCTGGAG AGCACCGACC GTGGGACCTG GGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_003435000.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003435048.1 Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OCe09393
Clone ID Related Accession (Same CDS sequence)	XM_003435000.3 , XM_003435000.2
Accession Version	XM_003435000.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase MRCK alpha isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003435000.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGCTA GCAAAGGCAG ACGTATTGTA GACAGTACTC CTCTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG TCCTGGTTCA ACTGGCTTTC CAGCTAAGCG CAAGACTCAT 
CAGTTTTTTG TCAAATCTTT CACAACACCT ACCAGATGTC ACCAGTGCAC CTCTCTGATG 
GTTGGTTTGA TAAGACAAGG CTGTTCCTGT GAGGTGTGCG GATTCTCATG TCATATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTCCCTCCTG AGCAGACAAA AGGTCCTCTG 
GGGATAGATC CACAGAAAGG AATAGGAACA GCCTATGAAG GTCATGTCAG GATTCCGAAA 
CCAGCTGGAG TAAAGAAAGG ATGGCAGAGA GCCCTGGCTG TGGTTTGTGA CTTTAAACTC 
TTCCTGTATG ATGTCGCTGA AGGAAAAGCA TCTCAGCCCA GCGTTGTGGT CAGTCAGGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTGT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTC AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
GACAGCAGGT GTTCGGTCCT AATGCTCGCA GAGAGTGAGA GCGAGAGGAA CAAGTGGGTG 
GGCGTGCTCA ACGAGCTGCA TAAGATTTTG AAGAAAAGCA AATTCAGAGA CCGCTCCGTC 
TATGTTCCCA AGGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCACGAAAG GATAGCTCTG GGCAATGAAG AAGGATTATT TGTTGTGCAC 
ATCACCAAAG ATGAAATTAT TAGAGTTGGT GATAATAAGA AGATACATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATATCAG CTTTGGACGG ACGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGG 
TGTCAGACCA TAACTTCGGG CAAAGTGTGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGGAAATTT 
AAGGAAATTC AAGTCCCATA TAACGTGCAA TGGATGGCGA TCTTCAGTGA ACAGCTCTGC 
GTGGGATTCC AGTCGGGATT CCTAAGATAC CCGTTGAACG GAGAGGGAAG CCCATACAGT 
ATGCTCCATT CAAATGACCA CACACTGTCA TTTATTGCCC ACCAGCCAAT GGATGCCATC 
TGCGCAGTTG AGATCTCCAG TAAAGAGTAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGGAG ATCGAGACAA CAGGAGTTGA TGTGGCCGTC AAATCCCTCC 
TCTTGTTGTT ACAACGCACC ATATCTCTCA GTGTATAGTG AGAATGCAGT TGATATCTTC 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG ACCCTTAAAC 
AGTGAAGGAT CATTAAACCT TTTAGGGCTG GAGACAATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTGGTGGTT CCTGAAACAT CGGACAATAG TCGGAAGCAG 
ATGGTTAGAA ACATCAACAA CAAGCGACGC TTCTCCTTCA GGGTCCCAGA AGAGGAGAGG 
ATGCAGCAGA GGAGAGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGCCCAGGGG ATGGAATACA GATCCTAAAA 
GACCTGCCTA TGAACCCTCG GCCTCAGGAA AGCCGGACCG TGTTCAGCGG CTCAGTCAGT 
ATTCCATCCA TCACTAAATC CCGCCCTGAG CCCGGCCGCT CCATGAGCGC CAGCAGCGGC 
CTTTCGGCAA GGTCATCTGC ACAGAATGGC AGTGCATTAA AGAGAGAATT CTCCGGAGGA 
AGCTATAATG CTAAGTGGCA GCCCATGCCC TCCCCATCAG AGGGCTCCTT GTCTTCCGGA 
GGCATGGACC AGGGAAGTGA CGCCCCAGCC AGGGACTATG ACGGAGAGGA CTCGGATTCT 
CCGAGGCACT CCACAGCTTC CAACAGCTCC AACCTCAGCA GCCCGCCCAG CCCAGTGTCA 
CCTCGGAAGA CCAAGAGCCT GTCCCTGGAG AGCACCGACC GTGGGACCTG GGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003435048.1
CDS	1..5100
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPASLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKTLKEEIENLRKQIRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIRTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGLCSIEHQQEISKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLYENVSMRNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGARATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKASKGRRIVDSTPLPAHTPTLRKKGCPGSTGFPAKRKTHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDVAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASDSRCSVLMLAESESERNKWVGVLNELHKILKKSKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHITKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPISALDGRETDFYKLAETKGCQTITSGKVCHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPSNPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRFSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNAKWQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGTWDP

Target ORF information:

RefSeq Version	XM_003435000.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003435000.3

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGCTA GCAAAGGCAG ACGTATTGTA GACAGTACTC CTCTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG TCCTGGTTCA ACTGGCTTTC CAGCTAAGCG CAAGACTCAT 
CAGTTTTTTG TCAAATCTTT CACAACACCT ACCAGATGTC ACCAGTGCAC CTCTCTGATG 
GTTGGTTTGA TAAGACAAGG CTGTTCCTGT GAGGTGTGCG GATTCTCATG TCATATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTCCCTCCTG AGCAGACAAA AGGTCCTCTG 
GGGATAGATC CACAGAAAGG AATAGGAACA GCCTATGAAG GTCATGTCAG GATTCCGAAA 
CCAGCTGGAG TAAAGAAAGG ATGGCAGAGA GCCCTGGCTG TGGTTTGTGA CTTTAAACTC 
TTCCTGTATG ATGTCGCTGA AGGAAAAGCA TCTCAGCCCA GCGTTGTGGT CAGTCAGGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTGT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTC AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
GACAGCAGGT GTTCGGTCCT AATGCTCGCA GAGAGTGAGA GCGAGAGGAA CAAGTGGGTG 
GGCGTGCTCA ACGAGCTGCA TAAGATTTTG AAGAAAAGCA AATTCAGAGA CCGCTCCGTC 
TATGTTCCCA AGGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCACGAAAG GATAGCTCTG GGCAATGAAG AAGGATTATT TGTTGTGCAC 
ATCACCAAAG ATGAAATTAT TAGAGTTGGT GATAATAAGA AGATACATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATATCAG CTTTGGACGG ACGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGG 
TGTCAGACCA TAACTTCGGG CAAAGTGTGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGGAAATTT 
AAGGAAATTC AAGTCCCATA TAACGTGCAA TGGATGGCGA TCTTCAGTGA ACAGCTCTGC 
GTGGGATTCC AGTCGGGATT CCTAAGATAC CCGTTGAACG GAGAGGGAAG CCCATACAGT 
ATGCTCCATT CAAATGACCA CACACTGTCA TTTATTGCCC ACCAGCCAAT GGATGCCATC 
TGCGCAGTTG AGATCTCCAG TAAAGAGTAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGGAG ATCGAGACAA CAGGAGTTGA TGTGGCCGTC AAATCCCTCC 
TCTTGTTGTT ACAACGCACC ATATCTCTCA GTGTATAGTG AGAATGCAGT TGATATCTTC 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG ACCCTTAAAC 
AGTGAAGGAT CATTAAACCT TTTAGGGCTG GAGACAATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTGGTGGTT CCTGAAACAT CGGACAATAG TCGGAAGCAG 
ATGGTTAGAA ACATCAACAA CAAGCGACGC TTCTCCTTCA GGGTCCCAGA AGAGGAGAGG 
ATGCAGCAGA GGAGAGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGCCCAGGGG ATGGAATACA GATCCTAAAA 
GACCTGCCTA TGAACCCTCG GCCTCAGGAA AGCCGGACCG TGTTCAGCGG CTCAGTCAGT 
ATTCCATCCA TCACTAAATC CCGCCCTGAG CCCGGCCGCT CCATGAGCGC CAGCAGCGGC 
CTTTCGGCAA GGTCATCTGC ACAGAATGGC AGTGCATTAA AGAGAGAATT CTCCGGAGGA 
AGCTATAATG CTAAGTGGCA GCCCATGCCC TCCCCATCAG AGGGCTCCTT GTCTTCCGGA 
GGCATGGACC AGGGAAGTGA CGCCCCAGCC AGGGACTATG ACGGAGAGGA CTCGGATTCT 
CCGAGGCACT CCACAGCTTC CAACAGCTCC AACCTCAGCA GCCCGCCCAG CCCAGTGTCA 
CCTCGGAAGA CCAAGAGCCT GTCCCTGGAG AGCACCGACC GTGGGACCTG GGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_003435000.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003435048.1 Canis lupus familiaris CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X16, mRNA.

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CloneID	OCe09393
Clone ID Related Accession (Same CDS sequence)	XM_003435000.3 , XM_003435000.2
Accession Version	XM_003435000.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase MRCK alpha isoform X15
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_003435000.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGCTA GCAAAGGCAG ACGTATTGTA GACAGTACTC CTCTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG TCCTGGTTCA ACTGGCTTTC CAGCTAAGCG CAAGACTCAT 
CAGTTTTTTG TCAAATCTTT CACAACACCT ACCAGATGTC ACCAGTGCAC CTCTCTGATG 
GTTGGTTTGA TAAGACAAGG CTGTTCCTGT GAGGTGTGCG GATTCTCATG TCATATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTCCCTCCTG AGCAGACAAA AGGTCCTCTG 
GGGATAGATC CACAGAAAGG AATAGGAACA GCCTATGAAG GTCATGTCAG GATTCCGAAA 
CCAGCTGGAG TAAAGAAAGG ATGGCAGAGA GCCCTGGCTG TGGTTTGTGA CTTTAAACTC 
TTCCTGTATG ATGTCGCTGA AGGAAAAGCA TCTCAGCCCA GCGTTGTGGT CAGTCAGGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTGT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTC AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
GACAGCAGGT GTTCGGTCCT AATGCTCGCA GAGAGTGAGA GCGAGAGGAA CAAGTGGGTG 
GGCGTGCTCA ACGAGCTGCA TAAGATTTTG AAGAAAAGCA AATTCAGAGA CCGCTCCGTC 
TATGTTCCCA AGGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCACGAAAG GATAGCTCTG GGCAATGAAG AAGGATTATT TGTTGTGCAC 
ATCACCAAAG ATGAAATTAT TAGAGTTGGT GATAATAAGA AGATACATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATATCAG CTTTGGACGG ACGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGG 
TGTCAGACCA TAACTTCGGG CAAAGTGTGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGGAAATTT 
AAGGAAATTC AAGTCCCATA TAACGTGCAA TGGATGGCGA TCTTCAGTGA ACAGCTCTGC 
GTGGGATTCC AGTCGGGATT CCTAAGATAC CCGTTGAACG GAGAGGGAAG CCCATACAGT 
ATGCTCCATT CAAATGACCA CACACTGTCA TTTATTGCCC ACCAGCCAAT GGATGCCATC 
TGCGCAGTTG AGATCTCCAG TAAAGAGTAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGGAG ATCGAGACAA CAGGAGTTGA TGTGGCCGTC AAATCCCTCC 
TCTTGTTGTT ACAACGCACC ATATCTCTCA GTGTATAGTG AGAATGCAGT TGATATCTTC 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG ACCCTTAAAC 
AGTGAAGGAT CATTAAACCT TTTAGGGCTG GAGACAATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTGGTGGTT CCTGAAACAT CGGACAATAG TCGGAAGCAG 
ATGGTTAGAA ACATCAACAA CAAGCGACGC TTCTCCTTCA GGGTCCCAGA AGAGGAGAGG 
ATGCAGCAGA GGAGAGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGCCCAGGGG ATGGAATACA GATCCTAAAA 
GACCTGCCTA TGAACCCTCG GCCTCAGGAA AGCCGGACCG TGTTCAGCGG CTCAGTCAGT 
ATTCCATCCA TCACTAAATC CCGCCCTGAG CCCGGCCGCT CCATGAGCGC CAGCAGCGGC 
CTTTCGGCAA GGTCATCTGC ACAGAATGGC AGTGCATTAA AGAGAGAATT CTCCGGAGGA 
AGCTATAATG CTAAGTGGCA GCCCATGCCC TCCCCATCAG AGGGCTCCTT GTCTTCCGGA 
GGCATGGACC AGGGAAGTGA CGCCCCAGCC AGGGACTATG ACGGAGAGGA CTCGGATTCT 
CCGAGGCACT CCACAGCTTC CAACAGCTCC AACCTCAGCA GCCCGCCCAG CCCAGTGTCA 
CCTCGGAAGA CCAAGAGCCT GTCCCTGGAG AGCACCGACC GTGGGACCTG GGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003435048.1
CDS	384..5483
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPASLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKTLKEEIENLRKQIRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIRTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGLCSIEHQQEISKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLYENVSMRNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGARATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKASKGRRIVDSTPLPAHTPTLRKKGCPGSTGFPAKRKTHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDVAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASDSRCSVLMLAESESERNKWVGVLNELHKILKKSKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHITKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPISALDGRETDFYKLAETKGCQTITSGKVCHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPSNPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRFSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNAKWQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGTWDP

Target ORF information:

RefSeq Version	XM_003435000.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003435000.2

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAGTCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAGAGAGC CAAAAAAGAG CTGGAAGTTC ATACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGATAGGAA ACTGCGTGAA CAGAGTGAGC ATTATTCTAA GCAACTGGAA 
AATGAATTGG AGGGTCTAAA GCAGAAACAA ATTAGTTACT CCCCAGGACT GTGCAGCATA 
GAACACCAGC AGGAGATATC CAAACTAAAG ACGGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAGGAAT TGTCTAAAAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTTCATG ATTCAGAAGG CCAGCAACTT GCTCTCAACA AGGAAATTAT GATTTTGAAA 
GACAAATTAG AGAAAAACAG AAGAGAGAGT CAAAGTGAAA GGGAAGAGTT TGAAAATGAA 
TTCAAACAAC AATATGAACG AGAAAAAGTA TTATTAACAG AAGAAAATAA AAAACTGACA 
AGTGAACTTG ATAAGCTCAC CACTTTGTAT GAAAATGTAA GTATGCGCAA CCAGCAGTTA 
GAAGAAGAGG TGAAAGACCT AGCAGACAAG AAAGAATCAG TTGCACATTG GGAAGCCCAA 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTCCAGGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTGAGGA ATTCCAGCTT GGGTGCACGA 
GCAACAGATA TGCCCTGGAA AATGCGTCGT TTTGCAAAAT TGGATATGTC AGCTAGACTG 
GAGTTGCAGT CAGCTCTGGA TGCAGAAATA AGAGCTAAAC AGGCCATCCA AGAAGAACTG 
AATAAAGTTA AAGCATCTAA CATCATCACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAGC TCCTCTCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGCTA GCAAAGGCAG ACGTATTGTA GACAGTACTC CTCTTCCAGC TCACACACCA 
ACCTTAAGGA AAAAGGGATG TCCTGGTTCA ACTGGCTTTC CAGCTAAGCG CAAGACTCAT 
CAGTTTTTTG TCAAATCTTT CACAACACCT ACCAGATGTC ACCAGTGCAC CTCTCTGATG 
GTTGGTTTGA TAAGACAAGG CTGTTCCTGT GAGGTGTGCG GATTCTCATG TCATATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTCCCTCCTG AGCAGACAAA AGGTCCTCTG 
GGGATAGATC CACAGAAAGG AATAGGAACA GCCTATGAAG GTCATGTCAG GATTCCGAAA 
CCAGCTGGAG TAAAGAAAGG ATGGCAGAGA GCCCTGGCTG TGGTTTGTGA CTTTAAACTC 
TTCCTGTATG ATGTCGCTGA AGGAAAAGCA TCTCAGCCCA GCGTTGTGGT CAGTCAGGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTGT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTC AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
GACAGCAGGT GTTCGGTCCT AATGCTCGCA GAGAGTGAGA GCGAGAGGAA CAAGTGGGTG 
GGCGTGCTCA ACGAGCTGCA TAAGATTTTG AAGAAAAGCA AATTCAGAGA CCGCTCCGTC 
TATGTTCCCA AGGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCACGAAAG GATAGCTCTG GGCAATGAAG AAGGATTATT TGTTGTGCAC 
ATCACCAAAG ATGAAATTAT TAGAGTTGGT GATAATAAGA AGATACATCA GATTGAACTC 
ATTCCAAATG ATCAGCTTGT TGCTGTGATC TCAGGACGAA ATCGTCATGT ACGACTTTTT 
CCTATATCAG CTTTGGACGG ACGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGG 
TGTCAGACCA TAACTTCGGG CAAAGTGTGC CATGGAGCTC TCACATGCCT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACCCGTCA CAGGAAATTT 
AAGGAAATTC AAGTCCCATA TAACGTGCAA TGGATGGCGA TCTTCAGTGA ACAGCTCTGC 
GTGGGATTCC AGTCGGGATT CCTAAGATAC CCGTTGAACG GAGAGGGAAG CCCATACAGT 
ATGCTCCATT CAAATGACCA CACACTGTCA TTTATTGCCC ACCAGCCAAT GGATGCCATC 
TGCGCAGTTG AGATCTCCAG TAAAGAGTAT CTGCTGTGTT TTAACAGCAT TGGGATATAC 
ACTGACTGCC AGGGCCGGAG ATCGAGACAA CAGGAGTTGA TGTGGCCGTC AAATCCCTCC 
TCTTGTTGTT ACAACGCACC ATATCTCTCA GTGTATAGTG AGAATGCAGT TGATATCTTC 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG ACCCTTAAAC 
AGTGAAGGAT CATTAAACCT TTTAGGGCTG GAGACAATTA GATTAATATA TTTCAAAAAT 
AAGATGGCAG AAGGGGATGA ACTGGTGGTT CCTGAAACAT CGGACAATAG TCGGAAGCAG 
ATGGTTAGAA ACATCAACAA CAAGCGACGC TTCTCCTTCA GGGTCCCAGA AGAGGAGAGG 
ATGCAGCAGA GGAGAGAGAT GCTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACACATG GGCCCAGGGG ATGGAATACA GATCCTAAAA 
GACCTGCCTA TGAACCCTCG GCCTCAGGAA AGCCGGACCG TGTTCAGCGG CTCAGTCAGT 
ATTCCATCCA TCACTAAATC CCGCCCTGAG CCCGGCCGCT CCATGAGCGC CAGCAGCGGC 
CTTTCGGCAA GGTCATCTGC ACAGAATGGC AGTGCATTAA AGAGAGAATT CTCCGGAGGA 
AGCTATAATG CTAAGTGGCA GCCCATGCCC TCCCCATCAG AGGGCTCCTT GTCTTCCGGA 
GGCATGGACC AGGGAAGTGA CGCCCCAGCC AGGGACTATG ACGGAGAGGA CTCGGATTCT 
CCGAGGCACT CCACAGCTTC CAACAGCTCC AACCTCAGCA GCCCGCCCAG CCCAGTGTCA 
CCTCGGAAGA CCAAGAGCCT GTCCCTGGAG AGCACCGACC GTGGGACCTG GGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_858483.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_863576.1 Canis lupus familiaris CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X17, mRNA.

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CloneID	OCe09394
Clone ID Related Accession (Same CDS sequence)	XM_858483.2 , XM_858483.3
Accession Version	XM_858483.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase MRCK alpha isoform X16
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_858483.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGCTG GAAGTTCATA CAGAAGCTGT GGCTGCTGAA 
GCATCTAAAG ATAGGAAACT GCGTGAACAG AGTGAGCATT ATTCTAAGCA ACTGGAAAAT 
GAATTGGAGG GTCTAAAGCA GAAACAAATT AGTTACTCCC CAGGACTGTG CAGCATAGAA 
CACCAGCAGG AGATATCCAA ACTAAAGACG GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAGGAATTGT CTAAAAGAGA AGGAATACAT GCAAATGAAA TTAAAAATCT GAAGAAAGAA 
CTTCATGATT CAGAAGGCCA GCAACTTGCT CTCAACAAGG AAATTATGAT TTTGAAAGAC 
AAATTAGAGA AAAACAGAAG AGAGAGTCAA AGTGAAAGGG AAGAGTTTGA AAATGAATTC 
AAACAACAAT ATGAACGAGA AAAAGTATTA TTAACAGAAG AAAATAAAAA ACTGACAAGT 
GAACTTGATA AGCTCACCAC TTTGTATGAA AATGTAAGTA TGCGCAACCA GCAGTTAGAA 
GAAGAGGTGA AAGACCTAGC AGACAAGAAA GAATCAGTTG CACATTGGGA AGCCCAAATC 
ACAGAAATAA TTCAGTGGGT CAGTGATGAA AAGGATGCAC GAGGGTATCT CCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAAGCT TTGAGGAATT CCAGCTTGGG TGCACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCAAAATTGG ATATGTCAGC TAGACTGGAG 
TTGCAGTCAG CTCTGGATGC AGAAATAAGA GCTAAACAGG CCATCCAAGA AGAACTGAAT 
AAAGTTAAAG CATCTAACAT CATCACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAGCTCC TCTCAGAAAT CGAACAACTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AATTGTAGAC AGTACTCCTC TTCCAGCTCA CACACCAACC 
TTAAGGAAAA AGGGATGTCC TGGTTCAACT GGCTTTCCAG CTAAGCGCAA GACTCATCAG 
TTTTTTGTCA AATCTTTCAC AACACCTACC AGATGTCACC AGTGCACCTC TCTGATGGTT 
GGTTTGATAA GACAAGGCTG TTCCTGTGAG GTGTGCGGAT TCTCATGTCA TATAACTTGT 
GTAAACAAAG CTCCAACTGC TTGTCCAGTC CCTCCTGAGC AGACAAAAGG TCCTCTGGGG 
ATAGATCCAC AGAAAGGAAT AGGAACAGCC TATGAAGGTC ATGTCAGGAT TCCGAAACCA 
GCTGGAGTAA AGAAAGGATG GCAGAGAGCC CTGGCTGTGG TTTGTGACTT TAAACTCTTC 
CTGTATGATG TCGCTGAAGG AAAAGCATCT CAGCCCAGCG TTGTGGTCAG TCAGGTGATT 
GACATGAGGG ATGAAGAATT TTCTGTGAGT TCAGTGTTGG CTTCTGATGT TATTCATGCA 
AGTCGAAAAG ATATACCCTG TATATTCAGA GTCACAGCTT CCCAGCTCTC AGCATCTGAC 
AGCAGGTGTT CGGTCCTAAT GCTCGCAGAG AGTGAGAGCG AGAGGAACAA GTGGGTGGGC 
GTGCTCAACG AGCTGCATAA GATTTTGAAG AAAAGCAAAT TCAGAGACCG CTCCGTCTAT 
GTTCCCAAGG AGGCTTATGA CAGCACTCTG CCCCTCATTA AAACAACCCA GGCAGCTGCA 
ATCATAGATC ACGAAAGGAT AGCTCTGGGC AATGAAGAAG GATTATTTGT TGTGCACATC 
ACCAAAGATG AAATTATTAG AGTTGGTGAT AATAAGAAGA TACATCAGAT TGAACTCATT 
CCAAATGATC AGCTTGTTGC TGTGATCTCA GGACGAAATC GTCATGTACG ACTTTTTCCT 
ATATCAGCTT TGGACGGACG AGAGACTGAT TTTTATAAGC TGGCAGAAAC TAAAGGGTGT 
CAGACCATAA CTTCGGGCAA AGTGTGCCAT GGAGCTCTCA CATGCCTGTG TGTGGCTATG 
AAAAGGCAGG TCCTCTGTTA TGAACTATTT CAGAGCAAGA CCCGTCACAG GAAATTTAAG 
GAAATTCAAG TCCCATATAA CGTGCAATGG ATGGCGATCT TCAGTGAACA GCTCTGCGTG 
GGATTCCAGT CGGGATTCCT AAGATACCCG TTGAACGGAG AGGGAAGCCC ATACAGTATG 
CTCCATTCAA ATGACCACAC ACTGTCATTT ATTGCCCACC AGCCAATGGA TGCCATCTGC 
GCAGTTGAGA TCTCCAGTAA AGAGTATCTG CTGTGTTTTA ACAGCATTGG GATATACACT 
GACTGCCAGG GCCGGAGATC GAGACAACAG GAGTTGATGT GGCCGTCAAA TCCCTCCTCT 
TGTTGTTACA ACGCACCATA TCTCTCAGTG TATAGTGAGA ATGCAGTTGA TATCTTCGAT 
GTGAACTCCA TGGAATGGAT TCAGACTCTC CCACTCAAAA AGGTTCGACC CTTAAACAGT 
GAAGGATCAT TAAACCTTTT AGGGCTGGAG ACAATTAGAT TAATATATTT CAAAAATAAG 
ATGGCAGAAG GGGATGAACT GGTGGTTCCT GAAACATCGG ACAATAGTCG GAAGCAGATG 
GTTAGAAACA TCAACAACAA GCGACGCTTC TCCTTCAGGG TCCCAGAAGA GGAGAGGATG 
CAGCAGAGGA GAGAGATGCT ACGAGATCCA GAAATGAGAA ATAAATTAAT TTCTAACCCA 
ACTAATTTTA ACCACATAGC ACACATGGGC CCAGGGGATG GAATACAGAT CCTAAAAGAC 
CTGCCTATGA ACCCTCGGCC TCAGGAAAGC CGGACCGTGT TCAGCGGCTC AGTCAGTATT 
CCATCCATCA CTAAATCCCG CCCTGAGCCC GGCCGCTCCA TGAGCGCCAG CAGCGGCCTT 
TCGGCAAGGT CATCTGCACA GAATGGCAGT GCATTAAAGA GAGAATTCTC CGGAGGAAGC 
TATAATGCTA AGTGGCAGCC CATGCCCTCC CCATCAGAGG GCTCCTTGTC TTCCGGAGGC 
ATGGACCAGG GAAGTGACGC CCCAGCCAGG GACTATGACG GAGAGGACTC GGATTCTCCG 
AGGCACTCCA CAGCTTCCAA CAGCTCCAAC CTCAGCAGCC CGCCCAGCCC AGTGTCACCT 
CGGAAGACCA AGAGCCTGTC CCTGGAGAGC ACCGACCGTG GGACCTGGGA CCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_863576.1
CDS	384..5300
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPASLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKTLKEEIENLRKQIRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIRTLQQEREELNKLEVHTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGLCSIEHQQEISKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLYENVSMRNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGARATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEIVDSTPLPAHTPTLRKKGCPGSTGFPAKRKTHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDVAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASDSRCSVLMLAESESERNKWVGVLNELHKILKKSKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHITKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPISALDGRETDFYKLAETKGCQTITSGKVCHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPSNPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRFSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNAKWQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGTWDP

Target ORF information:

RefSeq Version	XM_858483.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_858483.2

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGCTG GAAGTTCATA CAGAAGCTGT GGCTGCTGAA 
GCATCTAAAG ATAGGAAACT GCGTGAACAG AGTGAGCATT ATTCTAAGCA ACTGGAAAAT 
GAATTGGAGG GTCTAAAGCA GAAACAAATT AGTTACTCCC CAGGACTGTG CAGCATAGAA 
CACCAGCAGG AGATATCCAA ACTAAAGACG GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAGGAATTGT CTAAAAGAGA AGGAATACAT GCAAATGAAA TTAAAAATCT GAAGAAAGAA 
CTTCATGATT CAGAAGGCCA GCAACTTGCT CTCAACAAGG AAATTATGAT TTTGAAAGAC 
AAATTAGAGA AAAACAGAAG AGAGAGTCAA AGTGAAAGGG AAGAGTTTGA AAATGAATTC 
AAACAACAAT ATGAACGAGA AAAAGTATTA TTAACAGAAG AAAATAAAAA ACTGACAAGT 
GAACTTGATA AGCTCACCAC TTTGTATGAA AATGTAAGTA TGCGCAACCA GCAGTTAGAA 
GAAGAGGTGA AAGACCTAGC AGACAAGAAA GAATCAGTTG CACATTGGGA AGCCCAAATC 
ACAGAAATAA TTCAGTGGGT CAGTGATGAA AAGGATGCAC GAGGGTATCT CCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAAGCT TTGAGGAATT CCAGCTTGGG TGCACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCAAAATTGG ATATGTCAGC TAGACTGGAG 
TTGCAGTCAG CTCTGGATGC AGAAATAAGA GCTAAACAGG CCATCCAAGA AGAACTGAAT 
AAAGTTAAAG CATCTAACAT CATCACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAGCTCC TCTCAGAAAT CGAACAACTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AATTGTAGAC AGTACTCCTC TTCCAGCTCA CACACCAACC 
TTAAGGAAAA AGGGATGTCC TGGTTCAACT GGCTTTCCAG CTAAGCGCAA GACTCATCAG 
TTTTTTGTCA AATCTTTCAC AACACCTACC AGATGTCACC AGTGCACCTC TCTGATGGTT 
GGTTTGATAA GACAAGGCTG TTCCTGTGAG GTGTGCGGAT TCTCATGTCA TATAACTTGT 
GTAAACAAAG CTCCAACTGC TTGTCCAGTC CCTCCTGAGC AGACAAAAGG TCCTCTGGGG 
ATAGATCCAC AGAAAGGAAT AGGAACAGCC TATGAAGGTC ATGTCAGGAT TCCGAAACCA 
GCTGGAGTAA AGAAAGGATG GCAGAGAGCC CTGGCTGTGG TTTGTGACTT TAAACTCTTC 
CTGTATGATG TCGCTGAAGG AAAAGCATCT CAGCCCAGCG TTGTGGTCAG TCAGGTGATT 
GACATGAGGG ATGAAGAATT TTCTGTGAGT TCAGTGTTGG CTTCTGATGT TATTCATGCA 
AGTCGAAAAG ATATACCCTG TATATTCAGA GTCACAGCTT CCCAGCTCTC AGCATCTGAC 
AGCAGGTGTT CGGTCCTAAT GCTCGCAGAG AGTGAGAGCG AGAGGAACAA GTGGGTGGGC 
GTGCTCAACG AGCTGCATAA GATTTTGAAG AAAAGCAAAT TCAGAGACCG CTCCGTCTAT 
GTTCCCAAGG AGGCTTATGA CAGCACTCTG CCCCTCATTA AAACAACCCA GGCAGCTGCA 
ATCATAGATC ACGAAAGGAT AGCTCTGGGC AATGAAGAAG GATTATTTGT TGTGCACATC 
ACCAAAGATG AAATTATTAG AGTTGGTGAT AATAAGAAGA TACATCAGAT TGAACTCATT 
CCAAATGATC AGCTTGTTGC TGTGATCTCA GGACGAAATC GTCATGTACG ACTTTTTCCT 
ATATCAGCTT TGGACGGACG AGAGACTGAT TTTTATAAGC TGGCAGAAAC TAAAGGGTGT 
CAGACCATAA CTTCGGGCAA AGTGTGCCAT GGAGCTCTCA CATGCCTGTG TGTGGCTATG 
AAAAGGCAGG TCCTCTGTTA TGAACTATTT CAGAGCAAGA CCCGTCACAG GAAATTTAAG 
GAAATTCAAG TCCCATATAA CGTGCAATGG ATGGCGATCT TCAGTGAACA GCTCTGCGTG 
GGATTCCAGT CGGGATTCCT AAGATACCCG TTGAACGGAG AGGGAAGCCC ATACAGTATG 
CTCCATTCAA ATGACCACAC ACTGTCATTT ATTGCCCACC AGCCAATGGA TGCCATCTGC 
GCAGTTGAGA TCTCCAGTAA AGAGTATCTG CTGTGTTTTA ACAGCATTGG GATATACACT 
GACTGCCAGG GCCGGAGATC GAGACAACAG GAGTTGATGT GGCCGTCAAA TCCCTCCTCT 
TGTTGTTACA ACGCACCATA TCTCTCAGTG TATAGTGAGA ATGCAGTTGA TATCTTCGAT 
GTGAACTCCA TGGAATGGAT TCAGACTCTC CCACTCAAAA AGGTTCGACC CTTAAACAGT 
GAAGGATCAT TAAACCTTTT AGGGCTGGAG ACAATTAGAT TAATATATTT CAAAAATAAG 
ATGGCAGAAG GGGATGAACT GGTGGTTCCT GAAACATCGG ACAATAGTCG GAAGCAGATG 
GTTAGAAACA TCAACAACAA GCGACGCTTC TCCTTCAGGG TCCCAGAAGA GGAGAGGATG 
CAGCAGAGGA GAGAGATGCT ACGAGATCCA GAAATGAGAA ATAAATTAAT TTCTAACCCA 
ACTAATTTTA ACCACATAGC ACACATGGGC CCAGGGGATG GAATACAGAT CCTAAAAGAC 
CTGCCTATGA ACCCTCGGCC TCAGGAAAGC CGGACCGTGT TCAGCGGCTC AGTCAGTATT 
CCATCCATCA CTAAATCCCG CCCTGAGCCC GGCCGCTCCA TGAGCGCCAG CAGCGGCCTT 
TCGGCAAGGT CATCTGCACA GAATGGCAGT GCATTAAAGA GAGAATTCTC CGGAGGAAGC 
TATAATGCTA AGTGGCAGCC CATGCCCTCC CCATCAGAGG GCTCCTTGTC TTCCGGAGGC 
ATGGACCAGG GAAGTGACGC CCCAGCCAGG GACTATGACG GAGAGGACTC GGATTCTCCG 
AGGCACTCCA CAGCTTCCAA CAGCTCCAAC CTCAGCAGCC CGCCCAGCCC AGTGTCACCT 
CGGAAGACCA AGAGCCTGTC CCTGGAGAGC ACCGACCGTG GGACCTGGGA CCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_858483.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_863576.1 Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X21, mRNA.

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CloneID	OCe09394
Clone ID Related Accession (Same CDS sequence)	XM_858483.2 , XM_858483.3
Accession Version	XM_858483.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase MRCK alpha isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_858483.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGCTG GAAGTTCATA CAGAAGCTGT GGCTGCTGAA 
GCATCTAAAG ATAGGAAACT GCGTGAACAG AGTGAGCATT ATTCTAAGCA ACTGGAAAAT 
GAATTGGAGG GTCTAAAGCA GAAACAAATT AGTTACTCCC CAGGACTGTG CAGCATAGAA 
CACCAGCAGG AGATATCCAA ACTAAAGACG GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAGGAATTGT CTAAAAGAGA AGGAATACAT GCAAATGAAA TTAAAAATCT GAAGAAAGAA 
CTTCATGATT CAGAAGGCCA GCAACTTGCT CTCAACAAGG AAATTATGAT TTTGAAAGAC 
AAATTAGAGA AAAACAGAAG AGAGAGTCAA AGTGAAAGGG AAGAGTTTGA AAATGAATTC 
AAACAACAAT ATGAACGAGA AAAAGTATTA TTAACAGAAG AAAATAAAAA ACTGACAAGT 
GAACTTGATA AGCTCACCAC TTTGTATGAA AATGTAAGTA TGCGCAACCA GCAGTTAGAA 
GAAGAGGTGA AAGACCTAGC AGACAAGAAA GAATCAGTTG CACATTGGGA AGCCCAAATC 
ACAGAAATAA TTCAGTGGGT CAGTGATGAA AAGGATGCAC GAGGGTATCT CCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAAGCT TTGAGGAATT CCAGCTTGGG TGCACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCAAAATTGG ATATGTCAGC TAGACTGGAG 
TTGCAGTCAG CTCTGGATGC AGAAATAAGA GCTAAACAGG CCATCCAAGA AGAACTGAAT 
AAAGTTAAAG CATCTAACAT CATCACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAGCTCC TCTCAGAAAT CGAACAACTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AATTGTAGAC AGTACTCCTC TTCCAGCTCA CACACCAACC 
TTAAGGAAAA AGGGATGTCC TGGTTCAACT GGCTTTCCAG CTAAGCGCAA GACTCATCAG 
TTTTTTGTCA AATCTTTCAC AACACCTACC AGATGTCACC AGTGCACCTC TCTGATGGTT 
GGTTTGATAA GACAAGGCTG TTCCTGTGAG GTGTGCGGAT TCTCATGTCA TATAACTTGT 
GTAAACAAAG CTCCAACTGC TTGTCCAGTC CCTCCTGAGC AGACAAAAGG TCCTCTGGGG 
ATAGATCCAC AGAAAGGAAT AGGAACAGCC TATGAAGGTC ATGTCAGGAT TCCGAAACCA 
GCTGGAGTAA AGAAAGGATG GCAGAGAGCC CTGGCTGTGG TTTGTGACTT TAAACTCTTC 
CTGTATGATG TCGCTGAAGG AAAAGCATCT CAGCCCAGCG TTGTGGTCAG TCAGGTGATT 
GACATGAGGG ATGAAGAATT TTCTGTGAGT TCAGTGTTGG CTTCTGATGT TATTCATGCA 
AGTCGAAAAG ATATACCCTG TATATTCAGA GTCACAGCTT CCCAGCTCTC AGCATCTGAC 
AGCAGGTGTT CGGTCCTAAT GCTCGCAGAG AGTGAGAGCG AGAGGAACAA GTGGGTGGGC 
GTGCTCAACG AGCTGCATAA GATTTTGAAG AAAAGCAAAT TCAGAGACCG CTCCGTCTAT 
GTTCCCAAGG AGGCTTATGA CAGCACTCTG CCCCTCATTA AAACAACCCA GGCAGCTGCA 
ATCATAGATC ACGAAAGGAT AGCTCTGGGC AATGAAGAAG GATTATTTGT TGTGCACATC 
ACCAAAGATG AAATTATTAG AGTTGGTGAT AATAAGAAGA TACATCAGAT TGAACTCATT 
CCAAATGATC AGCTTGTTGC TGTGATCTCA GGACGAAATC GTCATGTACG ACTTTTTCCT 
ATATCAGCTT TGGACGGACG AGAGACTGAT TTTTATAAGC TGGCAGAAAC TAAAGGGTGT 
CAGACCATAA CTTCGGGCAA AGTGTGCCAT GGAGCTCTCA CATGCCTGTG TGTGGCTATG 
AAAAGGCAGG TCCTCTGTTA TGAACTATTT CAGAGCAAGA CCCGTCACAG GAAATTTAAG 
GAAATTCAAG TCCCATATAA CGTGCAATGG ATGGCGATCT TCAGTGAACA GCTCTGCGTG 
GGATTCCAGT CGGGATTCCT AAGATACCCG TTGAACGGAG AGGGAAGCCC ATACAGTATG 
CTCCATTCAA ATGACCACAC ACTGTCATTT ATTGCCCACC AGCCAATGGA TGCCATCTGC 
GCAGTTGAGA TCTCCAGTAA AGAGTATCTG CTGTGTTTTA ACAGCATTGG GATATACACT 
GACTGCCAGG GCCGGAGATC GAGACAACAG GAGTTGATGT GGCCGTCAAA TCCCTCCTCT 
TGTTGTTACA ACGCACCATA TCTCTCAGTG TATAGTGAGA ATGCAGTTGA TATCTTCGAT 
GTGAACTCCA TGGAATGGAT TCAGACTCTC CCACTCAAAA AGGTTCGACC CTTAAACAGT 
GAAGGATCAT TAAACCTTTT AGGGCTGGAG ACAATTAGAT TAATATATTT CAAAAATAAG 
ATGGCAGAAG GGGATGAACT GGTGGTTCCT GAAACATCGG ACAATAGTCG GAAGCAGATG 
GTTAGAAACA TCAACAACAA GCGACGCTTC TCCTTCAGGG TCCCAGAAGA GGAGAGGATG 
CAGCAGAGGA GAGAGATGCT ACGAGATCCA GAAATGAGAA ATAAATTAAT TTCTAACCCA 
ACTAATTTTA ACCACATAGC ACACATGGGC CCAGGGGATG GAATACAGAT CCTAAAAGAC 
CTGCCTATGA ACCCTCGGCC TCAGGAAAGC CGGACCGTGT TCAGCGGCTC AGTCAGTATT 
CCATCCATCA CTAAATCCCG CCCTGAGCCC GGCCGCTCCA TGAGCGCCAG CAGCGGCCTT 
TCGGCAAGGT CATCTGCACA GAATGGCAGT GCATTAAAGA GAGAATTCTC CGGAGGAAGC 
TATAATGCTA AGTGGCAGCC CATGCCCTCC CCATCAGAGG GCTCCTTGTC TTCCGGAGGC 
ATGGACCAGG GAAGTGACGC CCCAGCCAGG GACTATGACG GAGAGGACTC GGATTCTCCG 
AGGCACTCCA CAGCTTCCAA CAGCTCCAAC CTCAGCAGCC CGCCCAGCCC AGTGTCACCT 
CGGAAGACCA AGAGCCTGTC CCTGGAGAGC ACCGACCGTG GGACCTGGGA CCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_863576.1
CDS	1..4917
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPASLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKTLKEEIENLRKQIRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIRTLQQEREELNKLEVHTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGLCSIEHQQEISKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLYENVSMRNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGARATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEIVDSTPLPAHTPTLRKKGCPGSTGFPAKRKTHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDVAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASDSRCSVLMLAESESERNKWVGVLNELHKILKKSKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHITKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPISALDGRETDFYKLAETKGCQTITSGKVCHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPSNPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRFSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNAKWQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGTWDP

Target ORF information:

RefSeq Version	XM_858483.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_858483.3

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGCTT GAGGCAGTTG GAGCAGTTCA TTTTGGACGG GCCGGCGCAG 
ACCAATGGGC AGTGCTTCAG CGTGGAGACG CTGCTGGACA TCCTCATCTG CCTTTACGAT 
GAGTGCAACA ATTCTCCGCT GCGGAGGGAG AAGAACATTC TTGAATACCT GGAATGGGCC 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGCCTACATA GAGAAGACTT TGAGATATTG 
AAGGTGATAG GTCGAGGAGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGGGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGATA ATAAATGGAT TACAACTCTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACTTGGTCA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTGCTCAG CAAATTTGAA GATCGATTGC CTGAAGATAT GGCTCGATTT 
TACTTGGCCG AGATGGTGAT TGCAATCGAT TCTGTTCATC AGCTGCACTA TGTGCATAGG 
GACATCAAAC CAGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGATT AGCAGATTTT 
GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGGAAAGG AAGATACGGG 
CCTGAATGTG ACTGGTGGTC TCTGGGGGTC TGTATGTACG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAGACC TATGGAAAAA TCATGAATCA TAAAGAGAGG 
TTTCAGTTTC CAGCTCAAGT GACGGATGTG TCTGAAAATG CTAAAGATCT GATTCGTAGG 
CTCATTTGCA GCCGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAACAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC GTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACATCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC CACACATACT GCATTTTCTG GCCACCACCT GCCATTTGTT 
GGTTTCACAT ATACCAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG GGTCACGGCT 
GGTCCTGCGT CCCTGGACCT TGACGTCAGT GTCCAGCGGA CTCTAGACAA CAACCTGGCC 
ACTGAGGCAT ATGAGAGGAG AATTAAGCGC CTCGAACAGG AGAAGCTGGA GCTGAGTCGG 
AAGCTTCAAG AGTCAACACA GACTGTCCAA GCTCTACAGT ATTCAACTGC TGATGGCCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA ACCTTGAAAG AAGAAATTGA AAACCTAAGG 
AAACAAATAA GAGAATCAAG TCATTTGGAA CAACAACTTG AAGAAGCCAA CTCTGTGAGG 
AGAGAGTTAG ATGATGCTTT TCGACAAATC AAGGCTTATG AAAAACAAAT CAGAACACTT 
CAACAAGAAA GGGAAGAATT AAACAAGCTG GAAGTTCATA CAGAAGCTGT GGCTGCTGAA 
GCATCTAAAG ATAGGAAACT GCGTGAACAG AGTGAGCATT ATTCTAAGCA ACTGGAAAAT 
GAATTGGAGG GTCTAAAGCA GAAACAAATT AGTTACTCCC CAGGACTGTG CAGCATAGAA 
CACCAGCAGG AGATATCCAA ACTAAAGACG GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAGGAATTGT CTAAAAGAGA AGGAATACAT GCAAATGAAA TTAAAAATCT GAAGAAAGAA 
CTTCATGATT CAGAAGGCCA GCAACTTGCT CTCAACAAGG AAATTATGAT TTTGAAAGAC 
AAATTAGAGA AAAACAGAAG AGAGAGTCAA AGTGAAAGGG AAGAGTTTGA AAATGAATTC 
AAACAACAAT ATGAACGAGA AAAAGTATTA TTAACAGAAG AAAATAAAAA ACTGACAAGT 
GAACTTGATA AGCTCACCAC TTTGTATGAA AATGTAAGTA TGCGCAACCA GCAGTTAGAA 
GAAGAGGTGA AAGACCTAGC AGACAAGAAA GAATCAGTTG CACATTGGGA AGCCCAAATC 
ACAGAAATAA TTCAGTGGGT CAGTGATGAA AAGGATGCAC GAGGGTATCT CCAGGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAAGCT TTGAGGAATT CCAGCTTGGG TGCACGAGCA 
ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCAAAATTGG ATATGTCAGC TAGACTGGAG 
TTGCAGTCAG CTCTGGATGC AGAAATAAGA GCTAAACAGG CCATCCAAGA AGAACTGAAT 
AAAGTTAAAG CATCTAACAT CATCACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAGCTCC TCTCAGAAAT CGAACAACTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AATTGTAGAC AGTACTCCTC TTCCAGCTCA CACACCAACC 
TTAAGGAAAA AGGGATGTCC TGGTTCAACT GGCTTTCCAG CTAAGCGCAA GACTCATCAG 
TTTTTTGTCA AATCTTTCAC AACACCTACC AGATGTCACC AGTGCACCTC TCTGATGGTT 
GGTTTGATAA GACAAGGCTG TTCCTGTGAG GTGTGCGGAT TCTCATGTCA TATAACTTGT 
GTAAACAAAG CTCCAACTGC TTGTCCAGTC CCTCCTGAGC AGACAAAAGG TCCTCTGGGG 
ATAGATCCAC AGAAAGGAAT AGGAACAGCC TATGAAGGTC ATGTCAGGAT TCCGAAACCA 
GCTGGAGTAA AGAAAGGATG GCAGAGAGCC CTGGCTGTGG TTTGTGACTT TAAACTCTTC 
CTGTATGATG TCGCTGAAGG AAAAGCATCT CAGCCCAGCG TTGTGGTCAG TCAGGTGATT 
GACATGAGGG ATGAAGAATT TTCTGTGAGT TCAGTGTTGG CTTCTGATGT TATTCATGCA 
AGTCGAAAAG ATATACCCTG TATATTCAGA GTCACAGCTT CCCAGCTCTC AGCATCTGAC 
AGCAGGTGTT CGGTCCTAAT GCTCGCAGAG AGTGAGAGCG AGAGGAACAA GTGGGTGGGC 
GTGCTCAACG AGCTGCATAA GATTTTGAAG AAAAGCAAAT TCAGAGACCG CTCCGTCTAT 
GTTCCCAAGG AGGCTTATGA CAGCACTCTG CCCCTCATTA AAACAACCCA GGCAGCTGCA 
ATCATAGATC ACGAAAGGAT AGCTCTGGGC AATGAAGAAG GATTATTTGT TGTGCACATC 
ACCAAAGATG AAATTATTAG AGTTGGTGAT AATAAGAAGA TACATCAGAT TGAACTCATT 
CCAAATGATC AGCTTGTTGC TGTGATCTCA GGACGAAATC GTCATGTACG ACTTTTTCCT 
ATATCAGCTT TGGACGGACG AGAGACTGAT TTTTATAAGC TGGCAGAAAC TAAAGGGTGT 
CAGACCATAA CTTCGGGCAA AGTGTGCCAT GGAGCTCTCA CATGCCTGTG TGTGGCTATG 
AAAAGGCAGG TCCTCTGTTA TGAACTATTT CAGAGCAAGA CCCGTCACAG GAAATTTAAG 
GAAATTCAAG TCCCATATAA CGTGCAATGG ATGGCGATCT TCAGTGAACA GCTCTGCGTG 
GGATTCCAGT CGGGATTCCT AAGATACCCG TTGAACGGAG AGGGAAGCCC ATACAGTATG 
CTCCATTCAA ATGACCACAC ACTGTCATTT ATTGCCCACC AGCCAATGGA TGCCATCTGC 
GCAGTTGAGA TCTCCAGTAA AGAGTATCTG CTGTGTTTTA ACAGCATTGG GATATACACT 
GACTGCCAGG GCCGGAGATC GAGACAACAG GAGTTGATGT GGCCGTCAAA TCCCTCCTCT 
TGTTGTTACA ACGCACCATA TCTCTCAGTG TATAGTGAGA ATGCAGTTGA TATCTTCGAT 
GTGAACTCCA TGGAATGGAT TCAGACTCTC CCACTCAAAA AGGTTCGACC CTTAAACAGT 
GAAGGATCAT TAAACCTTTT AGGGCTGGAG ACAATTAGAT TAATATATTT CAAAAATAAG 
ATGGCAGAAG GGGATGAACT GGTGGTTCCT GAAACATCGG ACAATAGTCG GAAGCAGATG 
GTTAGAAACA TCAACAACAA GCGACGCTTC TCCTTCAGGG TCCCAGAAGA GGAGAGGATG 
CAGCAGAGGA GAGAGATGCT ACGAGATCCA GAAATGAGAA ATAAATTAAT TTCTAACCCA 
ACTAATTTTA ACCACATAGC ACACATGGGC CCAGGGGATG GAATACAGAT CCTAAAAGAC 
CTGCCTATGA ACCCTCGGCC TCAGGAAAGC CGGACCGTGT TCAGCGGCTC AGTCAGTATT 
CCATCCATCA CTAAATCCCG CCCTGAGCCC GGCCGCTCCA TGAGCGCCAG CAGCGGCCTT 
TCGGCAAGGT CATCTGCACA GAATGGCAGT GCATTAAAGA GAGAATTCTC CGGAGGAAGC 
TATAATGCTA AGTGGCAGCC CATGCCCTCC CCATCAGAGG GCTCCTTGTC TTCCGGAGGC 
ATGGACCAGG GAAGTGACGC CCCAGCCAGG GACTATGACG GAGAGGACTC GGATTCTCCG 
AGGCACTCCA CAGCTTCCAA CAGCTCCAAC CTCAGCAGCC CGCCCAGCCC AGTGTCACCT 
CGGAAGACCA AGAGCCTGTC CCTGGAGAGC ACCGACCGTG GGACCTGGGA CCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CDC42BPA
Entrez Gene ID	480107
Full Name	CDC42 binding protein kinase alpha
General protein information	Preferred Names CDC42 binding protein kinase alpha Names serine/threonine-protein kinase MRCK alpha
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Homo sapiens (human)	CDC42BPA	NP_003598.2
	CDC42BPA	XP_002801950.1
Canis lupus familiaris (dog)	CDC42BPA	XP_005622674.1
Gallus gallus (chicken)	CDC42BPA	NP_001186372.1
Xenopus tropicalis (tropical clawed frog)	cdc42bpa	XP_002938889.2
Pan troglodytes (chimpanzee)	CDC42BPA	XP_003949757.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012090	XP_320436.4
Rattus norvegicus (Norway rat)	Cdc42bpa	NP_446109.1
Danio rerio (zebrafish)	si:ch211-89p3.3	NP_001038559.1
Drosophila melanogaster (fruit fly)	gek	NP_523837.2
Caenorhabditis elegans (roundworm)	mrck-1	NP_504599.2
Mus musculus (house mouse)	Cdc42bpa	NP_001028457.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

CDC42BPA cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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見積依頼