Sema6d cDNA ORF clone, Mus musculus(house mouse)

The following Sema6d gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sema6d cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu72914 XM_017317026.1
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X30, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OMu72915 XM_017317028.1
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X31, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OMu20440 NM_199238.3
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $475.30
$679.00
OMu20440 NM_001290997.1
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $475.30
$679.00
OMu20440 XM_030249372.1
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu20440 XM_011239430.1
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $475.30
$679.00
OMu20440 XM_030249373.1
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu20434 XM_017317019.2
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu20449 XM_030249371.1
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu20449 XM_030249369.1
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu20490 XM_017317001.2
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu20490 XM_030249343.1
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu20490 XM_030249345.1
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu20528 XM_017317012.2
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu20528 XM_030249361.1
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu20528 XM_011239427.3
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu20528 XM_030249363.1
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu20556 XM_030249347.1
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu20556 XM_030249351.1
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu30226 XM_006499111.4
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu30226 XM_006499114.4
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu30226 XM_006499113.4
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu30226 XM_006499112.4
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu30226 XM_030249334.1
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu30226 XM_030249335.1
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu72911 XM_017316995.2
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu72912 XM_017317010.2
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu72912 XM_030249357.1
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu72913 XM_017317021.2
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu72913 XM_030249368.1
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu80174 XM_017317014.2
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu80174 XM_030249366.1
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu80174 XM_030249365.1
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu87775 XM_030249358.1
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu20434 NM_199239.3
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu20449 NM_172537.4
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $594.30
$849.00
OMu20490 NM_199241.3
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu20528 NM_199240.3
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu20556 NM_001291000.1
Latest version!
Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
View Old Accession Versions >>
OMu30226 XM_006499111.2 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu80174 XM_017317014.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu30226 XM_006499112.2 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu20434 XM_017317019.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu30226 XM_006499111.3 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu30226 XM_006499112.3 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu30226 XM_006499113.2 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu30226 XM_006499114.3 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu20490 XM_017317001.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu72912 XM_017317010.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu30226 XM_006499114.2 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu30226 XM_006499113.3 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu72913 XM_017317021.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu72911 XM_017316995.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu20528 XM_017317012.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu20528 XM_011239427.2 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu72914
    Clone ID Related Accession (Same CDS sequence) XM_017317026.1
    Accession Version XM_017317026.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1974bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG CAGCAACTCT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172515.1
    CDS832..2805
    Translation

    Target ORF information:

    RefSeq Version XM_017317026.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X30, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317026.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG CAGCAACTCT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu72915
    Clone ID Related Accession (Same CDS sequence) XM_017317028.1
    Accession Version XM_017317028.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG CAACTCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172517.1
    CDS832..2802
    Translation

    Target ORF information:

    RefSeq Version XM_017317028.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X31, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317028.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG CAACTCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20440
    Clone ID Related Accession (Same CDS sequence) XM_011239430.1 , NM_199238.3 , XM_030249372.1 , XM_030249373.1 , NM_001290997.1
    Accession Version NM_199238.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-10
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform Sema6D-2 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB091532.1, BC060680.1 and AL935323.6. On Mar 21, 2014 this sequence version replaced NM_199238.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (Sema6D-2) that is shorter than isoform Sema6D-1. Both variants 2 and 3 encode isoform Sema6D-2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC060680.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAATCC
    AATCAGATGG TCCACATGAA TGTCCTCATC ACCTGCGTGT TTGCCGCTTT TGTCTTGGGC
    GCGTTCATCG CAGGAGTGGC CGTGTACTGC TACCGTGACA TGTTCGTTCG GAAGAACAGA
    AAGATCCATA AAGACGCAGA ATCCGCCCAG TCGTGCACAG ACTCCAGCGG AAGCTTCGCC
    AAGCTGAACG GCCTCTTTGA CAGCCCCGTC AAGGAATACC AGCAGAACAT TGATTCTCCC
    AAACTCTACA GCAACCTGCT GACCAGTCGG AAGGAACTGC CACCAAACAC GGATACAAAG
    TCCATGGCCG TGGACCACAG AGGCCAGCCT CCCGAGCTGG CTGCTCTCCC CACGCCGGAA
    TCCACACCTG TCCTCCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC TGAGAAGGCC
    CACAGCCACG GTGCTTCAAG GAAAGAACAC CCCCAGTTTT TTCCTTCTAG TCCTCCACCC
    CATTCCCCAT TGAGTCACGG GCATATCCCC AGTGCCATCG TTCTTCCAAA CGCCACTCAC
    GACTACAATA CATCCTTCTC CAACTCGAAT GCCCACAAAG CCGAAAAGAA GCTTCAGAGC
    ATGGATCACC CTCTTACGAA GTCATCCAGT AAGCGGGAGC ACCGGCGGTC TGTGGATTCC
    AGGAATACTC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC
    ATCCTGGGAG AGATCCATAT GGCTCATCAA ACCCTCATGC TGGACCCGGT GGGACCAATG
    GCTGAGGTCC CACCCAAGGT CCCTAACCGG GAGGCATCTC TATACTCCCC TCCCTCCACA
    CTCCCCAGAA ATAGTCCAAC CAAGAGAGTA GATGTCCCCA CCACTCCTGG GGTGCCAATG
    ACTTCTCTGG AAAGACAAAG GGGTTATCAC AAAAATTCCT CCCAGAGGCA CTCTATATCT
    GCCGTGCCTA AAAACTTAAA CTCACCAAAT GGTGTTTTGT TATCTAGACA GCCGAGTATG
    AACCGTGGAG GCTATATGCC CACCCCAACA GGGGCGAAGG TGGACTATAT TCAGGGGACA
    CCGGTGAGTG TTCATCTGCA GCCCTCCCTC TCCAGACAGA GCAGCTATAC CAGTAATGGC
    ACCCTCCCCA GGACGGGACT AAAGAGGACA CCATCCTTAA AACCTGATGT GCCACCAAAG
    CCTTCCTTTG TTCCGCAAAC CACATCTGTC AGACCACTGA ACAAGTACAC GTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_954708.1
    CDS820..3816
    Translation

    Target ORF information:

    RefSeq Version NM_199238.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_199238.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAATCC
    AATCAGATGG TCCACATGAA TGTCCTCATC ACCTGCGTGT TTGCCGCTTT TGTCTTGGGC
    GCGTTCATCG CAGGAGTGGC CGTGTACTGC TACCGTGACA TGTTCGTTCG GAAGAACAGA
    AAGATCCATA AAGACGCAGA ATCCGCCCAG TCGTGCACAG ACTCCAGCGG AAGCTTCGCC
    AAGCTGAACG GCCTCTTTGA CAGCCCCGTC AAGGAATACC AGCAGAACAT TGATTCTCCC
    AAACTCTACA GCAACCTGCT GACCAGTCGG AAGGAACTGC CACCAAACAC GGATACAAAG
    TCCATGGCCG TGGACCACAG AGGCCAGCCT CCCGAGCTGG CTGCTCTCCC CACGCCGGAA
    TCCACACCTG TCCTCCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC TGAGAAGGCC
    CACAGCCACG GTGCTTCAAG GAAAGAACAC CCCCAGTTTT TTCCTTCTAG TCCTCCACCC
    CATTCCCCAT TGAGTCACGG GCATATCCCC AGTGCCATCG TTCTTCCAAA CGCCACTCAC
    GACTACAATA CATCCTTCTC CAACTCGAAT GCCCACAAAG CCGAAAAGAA GCTTCAGAGC
    ATGGATCACC CTCTTACGAA GTCATCCAGT AAGCGGGAGC ACCGGCGGTC TGTGGATTCC
    AGGAATACTC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC
    ATCCTGGGAG AGATCCATAT GGCTCATCAA ACCCTCATGC TGGACCCGGT GGGACCAATG
    GCTGAGGTCC CACCCAAGGT CCCTAACCGG GAGGCATCTC TATACTCCCC TCCCTCCACA
    CTCCCCAGAA ATAGTCCAAC CAAGAGAGTA GATGTCCCCA CCACTCCTGG GGTGCCAATG
    ACTTCTCTGG AAAGACAAAG GGGTTATCAC AAAAATTCCT CCCAGAGGCA CTCTATATCT
    GCCGTGCCTA AAAACTTAAA CTCACCAAAT GGTGTTTTGT TATCTAGACA GCCGAGTATG
    AACCGTGGAG GCTATATGCC CACCCCAACA GGGGCGAAGG TGGACTATAT TCAGGGGACA
    CCGGTGAGTG TTCATCTGCA GCCCTCCCTC TCCAGACAGA GCAGCTATAC CAGTAATGGC
    ACCCTCCCCA GGACGGGACT AAAGAGGACA CCATCCTTAA AACCTGATGT GCCACCAAAG
    CCTTCCTTTG TTCCGCAAAC CACATCTGTC AGACCACTGA ACAAGTACAC GTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20440
    Clone ID Related Accession (Same CDS sequence) XM_011239430.1 , NM_199238.3 , XM_030249372.1 , XM_030249373.1 , NM_001290997.1
    Accession Version NM_001290997.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-10
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform Sema6D-2 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL929570.7, BY242475.1, AL833808.4, BC060680.1 and AL935323.6. Transcript Variant: This variant (3) differs in the 5' UTR, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (Sema6D-2) is shorter than isoform Sema6D-1. Both variants 2 and 3 encode isoform Sema6D-2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK122515.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN01164132, SAMN01164136 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAATCC
    AATCAGATGG TCCACATGAA TGTCCTCATC ACCTGCGTGT TTGCCGCTTT TGTCTTGGGC
    GCGTTCATCG CAGGAGTGGC CGTGTACTGC TACCGTGACA TGTTCGTTCG GAAGAACAGA
    AAGATCCATA AAGACGCAGA ATCCGCCCAG TCGTGCACAG ACTCCAGCGG AAGCTTCGCC
    AAGCTGAACG GCCTCTTTGA CAGCCCCGTC AAGGAATACC AGCAGAACAT TGATTCTCCC
    AAACTCTACA GCAACCTGCT GACCAGTCGG AAGGAACTGC CACCAAACAC GGATACAAAG
    TCCATGGCCG TGGACCACAG AGGCCAGCCT CCCGAGCTGG CTGCTCTCCC CACGCCGGAA
    TCCACACCTG TCCTCCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC TGAGAAGGCC
    CACAGCCACG GTGCTTCAAG GAAAGAACAC CCCCAGTTTT TTCCTTCTAG TCCTCCACCC
    CATTCCCCAT TGAGTCACGG GCATATCCCC AGTGCCATCG TTCTTCCAAA CGCCACTCAC
    GACTACAATA CATCCTTCTC CAACTCGAAT GCCCACAAAG CCGAAAAGAA GCTTCAGAGC
    ATGGATCACC CTCTTACGAA GTCATCCAGT AAGCGGGAGC ACCGGCGGTC TGTGGATTCC
    AGGAATACTC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC
    ATCCTGGGAG AGATCCATAT GGCTCATCAA ACCCTCATGC TGGACCCGGT GGGACCAATG
    GCTGAGGTCC CACCCAAGGT CCCTAACCGG GAGGCATCTC TATACTCCCC TCCCTCCACA
    CTCCCCAGAA ATAGTCCAAC CAAGAGAGTA GATGTCCCCA CCACTCCTGG GGTGCCAATG
    ACTTCTCTGG AAAGACAAAG GGGTTATCAC AAAAATTCCT CCCAGAGGCA CTCTATATCT
    GCCGTGCCTA AAAACTTAAA CTCACCAAAT GGTGTTTTGT TATCTAGACA GCCGAGTATG
    AACCGTGGAG GCTATATGCC CACCCCAACA GGGGCGAAGG TGGACTATAT TCAGGGGACA
    CCGGTGAGTG TTCATCTGCA GCCCTCCCTC TCCAGACAGA GCAGCTATAC CAGTAATGGC
    ACCCTCCCCA GGACGGGACT AAAGAGGACA CCATCCTTAA AACCTGATGT GCCACCAAAG
    CCTTCCTTTG TTCCGCAAAC CACATCTGTC AGACCACTGA ACAAGTACAC GTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001277926.1
    CDS702..3698
    Translation

    Target ORF information:

    RefSeq Version NM_001290997.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001290997.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAATCC
    AATCAGATGG TCCACATGAA TGTCCTCATC ACCTGCGTGT TTGCCGCTTT TGTCTTGGGC
    GCGTTCATCG CAGGAGTGGC CGTGTACTGC TACCGTGACA TGTTCGTTCG GAAGAACAGA
    AAGATCCATA AAGACGCAGA ATCCGCCCAG TCGTGCACAG ACTCCAGCGG AAGCTTCGCC
    AAGCTGAACG GCCTCTTTGA CAGCCCCGTC AAGGAATACC AGCAGAACAT TGATTCTCCC
    AAACTCTACA GCAACCTGCT GACCAGTCGG AAGGAACTGC CACCAAACAC GGATACAAAG
    TCCATGGCCG TGGACCACAG AGGCCAGCCT CCCGAGCTGG CTGCTCTCCC CACGCCGGAA
    TCCACACCTG TCCTCCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC TGAGAAGGCC
    CACAGCCACG GTGCTTCAAG GAAAGAACAC CCCCAGTTTT TTCCTTCTAG TCCTCCACCC
    CATTCCCCAT TGAGTCACGG GCATATCCCC AGTGCCATCG TTCTTCCAAA CGCCACTCAC
    GACTACAATA CATCCTTCTC CAACTCGAAT GCCCACAAAG CCGAAAAGAA GCTTCAGAGC
    ATGGATCACC CTCTTACGAA GTCATCCAGT AAGCGGGAGC ACCGGCGGTC TGTGGATTCC
    AGGAATACTC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC
    ATCCTGGGAG AGATCCATAT GGCTCATCAA ACCCTCATGC TGGACCCGGT GGGACCAATG
    GCTGAGGTCC CACCCAAGGT CCCTAACCGG GAGGCATCTC TATACTCCCC TCCCTCCACA
    CTCCCCAGAA ATAGTCCAAC CAAGAGAGTA GATGTCCCCA CCACTCCTGG GGTGCCAATG
    ACTTCTCTGG AAAGACAAAG GGGTTATCAC AAAAATTCCT CCCAGAGGCA CTCTATATCT
    GCCGTGCCTA AAAACTTAAA CTCACCAAAT GGTGTTTTGT TATCTAGACA GCCGAGTATG
    AACCGTGGAG GCTATATGCC CACCCCAACA GGGGCGAAGG TGGACTATAT TCAGGGGACA
    CCGGTGAGTG TTCATCTGCA GCCCTCCCTC TCCAGACAGA GCAGCTATAC CAGTAATGGC
    ACCCTCCCCA GGACGGGACT AAAGAGGACA CCATCCTTAA AACCTGATGT GCCACCAAAG
    CCTTCCTTTG TTCCGCAAAC CACATCTGTC AGACCACTGA ACAAGTACAC GTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20440
    Clone ID Related Accession (Same CDS sequence) XM_011239430.1 , NM_199238.3 , XM_030249372.1 , XM_030249373.1 , NM_001290997.1
    Accession Version XM_011239430.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-02-08
    Organism Mus musculus(House mouse)
    Product semaphorin-6D isoform X4
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAATCC
    AATCAGATGG TCCACATGAA TGTCCTCATC ACCTGCGTGT TTGCCGCTTT TGTCTTGGGC
    GCGTTCATCG CAGGAGTGGC CGTGTACTGC TACCGTGACA TGTTCGTTCG GAAGAACAGA
    AAGATCCATA AAGACGCAGA ATCCGCCCAG TCGTGCACAG ACTCCAGCGG AAGCTTCGCC
    AAGCTGAACG GCCTCTTTGA CAGCCCCGTC AAGGAATACC AGCAGAACAT TGATTCTCCC
    AAACTCTACA GCAACCTGCT GACCAGTCGG AAGGAACTGC CACCAAACAC GGATACAAAG
    TCCATGGCCG TGGACCACAG AGGCCAGCCT CCCGAGCTGG CTGCTCTCCC CACGCCGGAA
    TCCACACCTG TCCTCCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC TGAGAAGGCC
    CACAGCCACG GTGCTTCAAG GAAAGAACAC CCCCAGTTTT TTCCTTCTAG TCCTCCACCC
    CATTCCCCAT TGAGTCACGG GCATATCCCC AGTGCCATCG TTCTTCCAAA CGCCACTCAC
    GACTACAATA CATCCTTCTC CAACTCGAAT GCCCACAAAG CCGAAAAGAA GCTTCAGAGC
    ATGGATCACC CTCTTACGAA GTCATCCAGT AAGCGGGAGC ACCGGCGGTC TGTGGATTCC
    AGGAATACTC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC
    ATCCTGGGAG AGATCCATAT GGCTCATCAA ACCCTCATGC TGGACCCGGT GGGACCAATG
    GCTGAGGTCC CACCCAAGGT CCCTAACCGG GAGGCATCTC TATACTCCCC TCCCTCCACA
    CTCCCCAGAA ATAGTCCAAC CAAGAGAGTA GATGTCCCCA CCACTCCTGG GGTGCCAATG
    ACTTCTCTGG AAAGACAAAG GGGTTATCAC AAAAATTCCT CCCAGAGGCA CTCTATATCT
    GCCGTGCCTA AAAACTTAAA CTCACCAAAT GGTGTTTTGT TATCTAGACA GCCGAGTATG
    AACCGTGGAG GCTATATGCC CACCCCAACA GGGGCGAAGG TGGACTATAT TCAGGGGACA
    CCGGTGAGTG TTCATCTGCA GCCCTCCCTC TCCAGACAGA GCAGCTATAC CAGTAATGGC
    ACCCTCCCCA GGACGGGACT AAAGAGGACA CCATCCTTAA AACCTGATGT GCCACCAAAG
    CCTTCCTTTG TTCCGCAAAC CACATCTGTC AGACCACTGA ACAAGTACAC GTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011237732.1
    Translation

    Target ORF information:

    RefSeq Version XM_011239430.1
    Organism Mus musculus(House mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011239430.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAATCC
    AATCAGATGG TCCACATGAA TGTCCTCATC ACCTGCGTGT TTGCCGCTTT TGTCTTGGGC
    GCGTTCATCG CAGGAGTGGC CGTGTACTGC TACCGTGACA TGTTCGTTCG GAAGAACAGA
    AAGATCCATA AAGACGCAGA ATCCGCCCAG TCGTGCACAG ACTCCAGCGG AAGCTTCGCC
    AAGCTGAACG GCCTCTTTGA CAGCCCCGTC AAGGAATACC AGCAGAACAT TGATTCTCCC
    AAACTCTACA GCAACCTGCT GACCAGTCGG AAGGAACTGC CACCAAACAC GGATACAAAG
    TCCATGGCCG TGGACCACAG AGGCCAGCCT CCCGAGCTGG CTGCTCTCCC CACGCCGGAA
    TCCACACCTG TCCTCCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC TGAGAAGGCC
    CACAGCCACG GTGCTTCAAG GAAAGAACAC CCCCAGTTTT TTCCTTCTAG TCCTCCACCC
    CATTCCCCAT TGAGTCACGG GCATATCCCC AGTGCCATCG TTCTTCCAAA CGCCACTCAC
    GACTACAATA CATCCTTCTC CAACTCGAAT GCCCACAAAG CCGAAAAGAA GCTTCAGAGC
    ATGGATCACC CTCTTACGAA GTCATCCAGT AAGCGGGAGC ACCGGCGGTC TGTGGATTCC
    AGGAATACTC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC
    ATCCTGGGAG AGATCCATAT GGCTCATCAA ACCCTCATGC TGGACCCGGT GGGACCAATG
    GCTGAGGTCC CACCCAAGGT CCCTAACCGG GAGGCATCTC TATACTCCCC TCCCTCCACA
    CTCCCCAGAA ATAGTCCAAC CAAGAGAGTA GATGTCCCCA CCACTCCTGG GGTGCCAATG
    ACTTCTCTGG AAAGACAAAG GGGTTATCAC AAAAATTCCT CCCAGAGGCA CTCTATATCT
    GCCGTGCCTA AAAACTTAAA CTCACCAAAT GGTGTTTTGT TATCTAGACA GCCGAGTATG
    AACCGTGGAG GCTATATGCC CACCCCAACA GGGGCGAAGG TGGACTATAT TCAGGGGACA
    CCGGTGAGTG TTCATCTGCA GCCCTCCCTC TCCAGACAGA GCAGCTATAC CAGTAATGGC
    ACCCTCCCCA GGACGGGACT AAAGAGGACA CCATCCTTAA AACCTGATGT GCCACCAAAG
    CCTTCCTTTG TTCCGCAAAC CACATCTGTC AGACCACTGA ACAAGTACAC GTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20440
    Clone ID Related Accession (Same CDS sequence) XM_011239430.1 , NM_199238.3 , XM_030249372.1 , XM_030249373.1 , NM_001290997.1
    Accession Version XM_030249372.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAATCC
    AATCAGATGG TCCACATGAA TGTCCTCATC ACCTGCGTGT TTGCCGCTTT TGTCTTGGGC
    GCGTTCATCG CAGGAGTGGC CGTGTACTGC TACCGTGACA TGTTCGTTCG GAAGAACAGA
    AAGATCCATA AAGACGCAGA ATCCGCCCAG TCGTGCACAG ACTCCAGCGG AAGCTTCGCC
    AAGCTGAACG GCCTCTTTGA CAGCCCCGTC AAGGAATACC AGCAGAACAT TGATTCTCCC
    AAACTCTACA GCAACCTGCT GACCAGTCGG AAGGAACTGC CACCAAACAC GGATACAAAG
    TCCATGGCCG TGGACCACAG AGGCCAGCCT CCCGAGCTGG CTGCTCTCCC CACGCCGGAA
    TCCACACCTG TCCTCCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC TGAGAAGGCC
    CACAGCCACG GTGCTTCAAG GAAAGAACAC CCCCAGTTTT TTCCTTCTAG TCCTCCACCC
    CATTCCCCAT TGAGTCACGG GCATATCCCC AGTGCCATCG TTCTTCCAAA CGCCACTCAC
    GACTACAATA CATCCTTCTC CAACTCGAAT GCCCACAAAG CCGAAAAGAA GCTTCAGAGC
    ATGGATCACC CTCTTACGAA GTCATCCAGT AAGCGGGAGC ACCGGCGGTC TGTGGATTCC
    AGGAATACTC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC
    ATCCTGGGAG AGATCCATAT GGCTCATCAA ACCCTCATGC TGGACCCGGT GGGACCAATG
    GCTGAGGTCC CACCCAAGGT CCCTAACCGG GAGGCATCTC TATACTCCCC TCCCTCCACA
    CTCCCCAGAA ATAGTCCAAC CAAGAGAGTA GATGTCCCCA CCACTCCTGG GGTGCCAATG
    ACTTCTCTGG AAAGACAAAG GGGTTATCAC AAAAATTCCT CCCAGAGGCA CTCTATATCT
    GCCGTGCCTA AAAACTTAAA CTCACCAAAT GGTGTTTTGT TATCTAGACA GCCGAGTATG
    AACCGTGGAG GCTATATGCC CACCCCAACA GGGGCGAAGG TGGACTATAT TCAGGGGACA
    CCGGTGAGTG TTCATCTGCA GCCCTCCCTC TCCAGACAGA GCAGCTATAC CAGTAATGGC
    ACCCTCCCCA GGACGGGACT AAAGAGGACA CCATCCTTAA AACCTGATGT GCCACCAAAG
    CCTTCCTTTG TTCCGCAAAC CACATCTGTC AGACCACTGA ACAAGTACAC GTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030105232.1
    CDS605..3601
    Translation

    Target ORF information:

    RefSeq Version XM_030249372.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249372.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAATCC
    AATCAGATGG TCCACATGAA TGTCCTCATC ACCTGCGTGT TTGCCGCTTT TGTCTTGGGC
    GCGTTCATCG CAGGAGTGGC CGTGTACTGC TACCGTGACA TGTTCGTTCG GAAGAACAGA
    AAGATCCATA AAGACGCAGA ATCCGCCCAG TCGTGCACAG ACTCCAGCGG AAGCTTCGCC
    AAGCTGAACG GCCTCTTTGA CAGCCCCGTC AAGGAATACC AGCAGAACAT TGATTCTCCC
    AAACTCTACA GCAACCTGCT GACCAGTCGG AAGGAACTGC CACCAAACAC GGATACAAAG
    TCCATGGCCG TGGACCACAG AGGCCAGCCT CCCGAGCTGG CTGCTCTCCC CACGCCGGAA
    TCCACACCTG TCCTCCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC TGAGAAGGCC
    CACAGCCACG GTGCTTCAAG GAAAGAACAC CCCCAGTTTT TTCCTTCTAG TCCTCCACCC
    CATTCCCCAT TGAGTCACGG GCATATCCCC AGTGCCATCG TTCTTCCAAA CGCCACTCAC
    GACTACAATA CATCCTTCTC CAACTCGAAT GCCCACAAAG CCGAAAAGAA GCTTCAGAGC
    ATGGATCACC CTCTTACGAA GTCATCCAGT AAGCGGGAGC ACCGGCGGTC TGTGGATTCC
    AGGAATACTC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC
    ATCCTGGGAG AGATCCATAT GGCTCATCAA ACCCTCATGC TGGACCCGGT GGGACCAATG
    GCTGAGGTCC CACCCAAGGT CCCTAACCGG GAGGCATCTC TATACTCCCC TCCCTCCACA
    CTCCCCAGAA ATAGTCCAAC CAAGAGAGTA GATGTCCCCA CCACTCCTGG GGTGCCAATG
    ACTTCTCTGG AAAGACAAAG GGGTTATCAC AAAAATTCCT CCCAGAGGCA CTCTATATCT
    GCCGTGCCTA AAAACTTAAA CTCACCAAAT GGTGTTTTGT TATCTAGACA GCCGAGTATG
    AACCGTGGAG GCTATATGCC CACCCCAACA GGGGCGAAGG TGGACTATAT TCAGGGGACA
    CCGGTGAGTG TTCATCTGCA GCCCTCCCTC TCCAGACAGA GCAGCTATAC CAGTAATGGC
    ACCCTCCCCA GGACGGGACT AAAGAGGACA CCATCCTTAA AACCTGATGT GCCACCAAAG
    CCTTCCTTTG TTCCGCAAAC CACATCTGTC AGACCACTGA ACAAGTACAC GTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20440
    Clone ID Related Accession (Same CDS sequence) XM_011239430.1 , NM_199238.3 , XM_030249372.1 , XM_030249373.1 , NM_001290997.1
    Accession Version XM_030249373.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAATCC
    AATCAGATGG TCCACATGAA TGTCCTCATC ACCTGCGTGT TTGCCGCTTT TGTCTTGGGC
    GCGTTCATCG CAGGAGTGGC CGTGTACTGC TACCGTGACA TGTTCGTTCG GAAGAACAGA
    AAGATCCATA AAGACGCAGA ATCCGCCCAG TCGTGCACAG ACTCCAGCGG AAGCTTCGCC
    AAGCTGAACG GCCTCTTTGA CAGCCCCGTC AAGGAATACC AGCAGAACAT TGATTCTCCC
    AAACTCTACA GCAACCTGCT GACCAGTCGG AAGGAACTGC CACCAAACAC GGATACAAAG
    TCCATGGCCG TGGACCACAG AGGCCAGCCT CCCGAGCTGG CTGCTCTCCC CACGCCGGAA
    TCCACACCTG TCCTCCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC TGAGAAGGCC
    CACAGCCACG GTGCTTCAAG GAAAGAACAC CCCCAGTTTT TTCCTTCTAG TCCTCCACCC
    CATTCCCCAT TGAGTCACGG GCATATCCCC AGTGCCATCG TTCTTCCAAA CGCCACTCAC
    GACTACAATA CATCCTTCTC CAACTCGAAT GCCCACAAAG CCGAAAAGAA GCTTCAGAGC
    ATGGATCACC CTCTTACGAA GTCATCCAGT AAGCGGGAGC ACCGGCGGTC TGTGGATTCC
    AGGAATACTC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC
    ATCCTGGGAG AGATCCATAT GGCTCATCAA ACCCTCATGC TGGACCCGGT GGGACCAATG
    GCTGAGGTCC CACCCAAGGT CCCTAACCGG GAGGCATCTC TATACTCCCC TCCCTCCACA
    CTCCCCAGAA ATAGTCCAAC CAAGAGAGTA GATGTCCCCA CCACTCCTGG GGTGCCAATG
    ACTTCTCTGG AAAGACAAAG GGGTTATCAC AAAAATTCCT CCCAGAGGCA CTCTATATCT
    GCCGTGCCTA AAAACTTAAA CTCACCAAAT GGTGTTTTGT TATCTAGACA GCCGAGTATG
    AACCGTGGAG GCTATATGCC CACCCCAACA GGGGCGAAGG TGGACTATAT TCAGGGGACA
    CCGGTGAGTG TTCATCTGCA GCCCTCCCTC TCCAGACAGA GCAGCTATAC CAGTAATGGC
    ACCCTCCCCA GGACGGGACT AAAGAGGACA CCATCCTTAA AACCTGATGT GCCACCAAAG
    CCTTCCTTTG TTCCGCAAAC CACATCTGTC AGACCACTGA ACAAGTACAC GTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030105233.1
    CDS8748..11744
    Translation

    Target ORF information:

    RefSeq Version XM_030249373.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X29, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249373.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAATCC
    AATCAGATGG TCCACATGAA TGTCCTCATC ACCTGCGTGT TTGCCGCTTT TGTCTTGGGC
    GCGTTCATCG CAGGAGTGGC CGTGTACTGC TACCGTGACA TGTTCGTTCG GAAGAACAGA
    AAGATCCATA AAGACGCAGA ATCCGCCCAG TCGTGCACAG ACTCCAGCGG AAGCTTCGCC
    AAGCTGAACG GCCTCTTTGA CAGCCCCGTC AAGGAATACC AGCAGAACAT TGATTCTCCC
    AAACTCTACA GCAACCTGCT GACCAGTCGG AAGGAACTGC CACCAAACAC GGATACAAAG
    TCCATGGCCG TGGACCACAG AGGCCAGCCT CCCGAGCTGG CTGCTCTCCC CACGCCGGAA
    TCCACACCTG TCCTCCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC TGAGAAGGCC
    CACAGCCACG GTGCTTCAAG GAAAGAACAC CCCCAGTTTT TTCCTTCTAG TCCTCCACCC
    CATTCCCCAT TGAGTCACGG GCATATCCCC AGTGCCATCG TTCTTCCAAA CGCCACTCAC
    GACTACAATA CATCCTTCTC CAACTCGAAT GCCCACAAAG CCGAAAAGAA GCTTCAGAGC
    ATGGATCACC CTCTTACGAA GTCATCCAGT AAGCGGGAGC ACCGGCGGTC TGTGGATTCC
    AGGAATACTC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC
    ATCCTGGGAG AGATCCATAT GGCTCATCAA ACCCTCATGC TGGACCCGGT GGGACCAATG
    GCTGAGGTCC CACCCAAGGT CCCTAACCGG GAGGCATCTC TATACTCCCC TCCCTCCACA
    CTCCCCAGAA ATAGTCCAAC CAAGAGAGTA GATGTCCCCA CCACTCCTGG GGTGCCAATG
    ACTTCTCTGG AAAGACAAAG GGGTTATCAC AAAAATTCCT CCCAGAGGCA CTCTATATCT
    GCCGTGCCTA AAAACTTAAA CTCACCAAAT GGTGTTTTGT TATCTAGACA GCCGAGTATG
    AACCGTGGAG GCTATATGCC CACCCCAACA GGGGCGAAGG TGGACTATAT TCAGGGGACA
    CCGGTGAGTG TTCATCTGCA GCCCTCCCTC TCCAGACAGA GCAGCTATAC CAGTAATGGC
    ACCCTCCCCA GGACGGGACT AAAGAGGACA CCATCCTTAA AACCTGATGT GCCACCAAAG
    CCTTCCTTTG TTCCGCAAAC CACATCTGTC AGACCACTGA ACAAGTACAC GTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20434
    Clone ID Related Accession (Same CDS sequence) XM_017317019.2 , XM_017317019.1 , NM_199239.3
    Accession Version XM_017317019.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3093bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317019.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC
    CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG
    TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC
    GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC
    CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC
    AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC
    CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG
    ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA
    GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT
    ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC
    TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA
    TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG
    AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT
    CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT
    AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG
    AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT
    TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA
    CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC
    CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC
    TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG
    AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA
    TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172508.1
    CDS830..3922
    Translation

    Target ORF information:

    RefSeq Version XM_017317019.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317019.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC
    CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG
    TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC
    GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC
    CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC
    AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC
    CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG
    ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA
    GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT
    ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC
    TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA
    TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG
    AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT
    CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT
    AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG
    AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT
    TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA
    CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC
    CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC
    TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG
    AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA
    TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20449
    Clone ID Related Accession (Same CDS sequence) XM_030249369.1 , XM_030249371.1 , NM_172537.4
    Accession Version XM_030249369.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGGTG TACGGTGGGA AGTCCAGTCT GGAGAATCCA ATCAGATGGT CCACATGAAT
    GTCCTCATCA CCTGCGTGTT TGCCGCTTTT GTCTTGGGCG CGTTCATCGC AGGAGTGGCC
    GTGTACTGCT ACCGTGACAT GTTCGTTCGG AAGAACAGAA AGATCCATAA AGACGCAGAA
    TCCGCCCAGT CGTGCACAGA CTCCAGCGGA AGCTTCGCCA AGCTGAACGG CCTCTTTGAC
    AGCCCCGTCA AGGAATACCA GCAGAACATT GATTCTCCCA AACTCTACAG CAACCTGCTG
    ACCAGTCGGA AGGAACTGCC ACCAAACACG GATACAAAGT CCATGGCCGT GGACCACAGA
    GGCCAGCCTC CCGAGCTGGC TGCTCTCCCC ACGCCGGAAT CCACACCTGT CCTCCACCAG
    AAGACCCTGC AGGCCATGAA GAGCCACTCT GAGAAGGCCC ACAGCCACGG TGCTTCAAGG
    AAAGAACACC CCCAGTTTTT TCCTTCTAGT CCTCCACCCC ATTCCCCATT GAGTCACGGG
    CATATCCCCA GTGCCATCGT TCTTCCAAAC GCCACTCACG ACTACAATAC ATCCTTCTCC
    AACTCGAATG CCCACAAAGC CGAAAAGAAG CTTCAGAGCA TGGATCACCC TCTTACGAAG
    TCATCCAGTA AGCGGGAGCA CCGGCGGTCT GTGGATTCCA GGAATACTCT CAATGATCTC
    CTGAAGCATC TAAATGACCC AAACAGTAAC CCCAAAGCCA TCCTGGGAGA GATCCATATG
    GCTCATCAAA CCCTCATGCT GGACCCGGTG GGACCAATGG CTGAGGTCCC ACCCAAGGTC
    CCTAACCGGG AGGCATCTCT ATACTCCCCT CCCTCCACAC TCCCCAGAAA TAGTCCAACC
    AAGAGAGTAG ATGTCCCCAC CACTCCTGGG GTGCCAATGA CTTCTCTGGA AAGACAAAGG
    GGTTATCACA AAAATTCCTC CCAGAGGCAC TCTATATCTG CCGTGCCTAA AAACTTAAAC
    TCACCAAATG GTGTTTTGTT ATCTAGACAG CCGAGTATGA ACCGTGGAGG CTATATGCCC
    ACCCCAACAG GGGCGAAGGT GGACTATATT CAGGGGACAC CGGTGAGTGT TCATCTGCAG
    CCCTCCCTCT CCAGACAGAG CAGCTATACC AGTAATGGCA CCCTCCCCAG GACGGGACTA
    AAGAGGACAC CATCCTTAAA ACCTGATGTG CCACCAAAGC CTTCCTTTGT TCCGCAAACC
    ACATCTGTCA GACCACTGAA CAAGTACACG TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030105229.1
    CDS605..3640
    Translation

    Target ORF information:

    RefSeq Version XM_030249369.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249369.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGGTG TACGGTGGGA AGTCCAGTCT GGAGAATCCA ATCAGATGGT CCACATGAAT
    GTCCTCATCA CCTGCGTGTT TGCCGCTTTT GTCTTGGGCG CGTTCATCGC AGGAGTGGCC
    GTGTACTGCT ACCGTGACAT GTTCGTTCGG AAGAACAGAA AGATCCATAA AGACGCAGAA
    TCCGCCCAGT CGTGCACAGA CTCCAGCGGA AGCTTCGCCA AGCTGAACGG CCTCTTTGAC
    AGCCCCGTCA AGGAATACCA GCAGAACATT GATTCTCCCA AACTCTACAG CAACCTGCTG
    ACCAGTCGGA AGGAACTGCC ACCAAACACG GATACAAAGT CCATGGCCGT GGACCACAGA
    GGCCAGCCTC CCGAGCTGGC TGCTCTCCCC ACGCCGGAAT CCACACCTGT CCTCCACCAG
    AAGACCCTGC AGGCCATGAA GAGCCACTCT GAGAAGGCCC ACAGCCACGG TGCTTCAAGG
    AAAGAACACC CCCAGTTTTT TCCTTCTAGT CCTCCACCCC ATTCCCCATT GAGTCACGGG
    CATATCCCCA GTGCCATCGT TCTTCCAAAC GCCACTCACG ACTACAATAC ATCCTTCTCC
    AACTCGAATG CCCACAAAGC CGAAAAGAAG CTTCAGAGCA TGGATCACCC TCTTACGAAG
    TCATCCAGTA AGCGGGAGCA CCGGCGGTCT GTGGATTCCA GGAATACTCT CAATGATCTC
    CTGAAGCATC TAAATGACCC AAACAGTAAC CCCAAAGCCA TCCTGGGAGA GATCCATATG
    GCTCATCAAA CCCTCATGCT GGACCCGGTG GGACCAATGG CTGAGGTCCC ACCCAAGGTC
    CCTAACCGGG AGGCATCTCT ATACTCCCCT CCCTCCACAC TCCCCAGAAA TAGTCCAACC
    AAGAGAGTAG ATGTCCCCAC CACTCCTGGG GTGCCAATGA CTTCTCTGGA AAGACAAAGG
    GGTTATCACA AAAATTCCTC CCAGAGGCAC TCTATATCTG CCGTGCCTAA AAACTTAAAC
    TCACCAAATG GTGTTTTGTT ATCTAGACAG CCGAGTATGA ACCGTGGAGG CTATATGCCC
    ACCCCAACAG GGGCGAAGGT GGACTATATT CAGGGGACAC CGGTGAGTGT TCATCTGCAG
    CCCTCCCTCT CCAGACAGAG CAGCTATACC AGTAATGGCA CCCTCCCCAG GACGGGACTA
    AAGAGGACAC CATCCTTAAA ACCTGATGTG CCACCAAAGC CTTCCTTTGT TCCGCAAACC
    ACATCTGTCA GACCACTGAA CAAGTACACG TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20449
    Clone ID Related Accession (Same CDS sequence) XM_030249369.1 , XM_030249371.1 , NM_172537.4
    Accession Version XM_030249371.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGGTG TACGGTGGGA AGTCCAGTCT GGAGAATCCA ATCAGATGGT CCACATGAAT
    GTCCTCATCA CCTGCGTGTT TGCCGCTTTT GTCTTGGGCG CGTTCATCGC AGGAGTGGCC
    GTGTACTGCT ACCGTGACAT GTTCGTTCGG AAGAACAGAA AGATCCATAA AGACGCAGAA
    TCCGCCCAGT CGTGCACAGA CTCCAGCGGA AGCTTCGCCA AGCTGAACGG CCTCTTTGAC
    AGCCCCGTCA AGGAATACCA GCAGAACATT GATTCTCCCA AACTCTACAG CAACCTGCTG
    ACCAGTCGGA AGGAACTGCC ACCAAACACG GATACAAAGT CCATGGCCGT GGACCACAGA
    GGCCAGCCTC CCGAGCTGGC TGCTCTCCCC ACGCCGGAAT CCACACCTGT CCTCCACCAG
    AAGACCCTGC AGGCCATGAA GAGCCACTCT GAGAAGGCCC ACAGCCACGG TGCTTCAAGG
    AAAGAACACC CCCAGTTTTT TCCTTCTAGT CCTCCACCCC ATTCCCCATT GAGTCACGGG
    CATATCCCCA GTGCCATCGT TCTTCCAAAC GCCACTCACG ACTACAATAC ATCCTTCTCC
    AACTCGAATG CCCACAAAGC CGAAAAGAAG CTTCAGAGCA TGGATCACCC TCTTACGAAG
    TCATCCAGTA AGCGGGAGCA CCGGCGGTCT GTGGATTCCA GGAATACTCT CAATGATCTC
    CTGAAGCATC TAAATGACCC AAACAGTAAC CCCAAAGCCA TCCTGGGAGA GATCCATATG
    GCTCATCAAA CCCTCATGCT GGACCCGGTG GGACCAATGG CTGAGGTCCC ACCCAAGGTC
    CCTAACCGGG AGGCATCTCT ATACTCCCCT CCCTCCACAC TCCCCAGAAA TAGTCCAACC
    AAGAGAGTAG ATGTCCCCAC CACTCCTGGG GTGCCAATGA CTTCTCTGGA AAGACAAAGG
    GGTTATCACA AAAATTCCTC CCAGAGGCAC TCTATATCTG CCGTGCCTAA AAACTTAAAC
    TCACCAAATG GTGTTTTGTT ATCTAGACAG CCGAGTATGA ACCGTGGAGG CTATATGCCC
    ACCCCAACAG GGGCGAAGGT GGACTATATT CAGGGGACAC CGGTGAGTGT TCATCTGCAG
    CCCTCCCTCT CCAGACAGAG CAGCTATACC AGTAATGGCA CCCTCCCCAG GACGGGACTA
    AAGAGGACAC CATCCTTAAA ACCTGATGTG CCACCAAAGC CTTCCTTTGT TCCGCAAACC
    ACATCTGTCA GACCACTGAA CAAGTACACG TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030105231.1
    CDS8748..11783
    Translation

    Target ORF information:

    RefSeq Version XM_030249371.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249371.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGGTG TACGGTGGGA AGTCCAGTCT GGAGAATCCA ATCAGATGGT CCACATGAAT
    GTCCTCATCA CCTGCGTGTT TGCCGCTTTT GTCTTGGGCG CGTTCATCGC AGGAGTGGCC
    GTGTACTGCT ACCGTGACAT GTTCGTTCGG AAGAACAGAA AGATCCATAA AGACGCAGAA
    TCCGCCCAGT CGTGCACAGA CTCCAGCGGA AGCTTCGCCA AGCTGAACGG CCTCTTTGAC
    AGCCCCGTCA AGGAATACCA GCAGAACATT GATTCTCCCA AACTCTACAG CAACCTGCTG
    ACCAGTCGGA AGGAACTGCC ACCAAACACG GATACAAAGT CCATGGCCGT GGACCACAGA
    GGCCAGCCTC CCGAGCTGGC TGCTCTCCCC ACGCCGGAAT CCACACCTGT CCTCCACCAG
    AAGACCCTGC AGGCCATGAA GAGCCACTCT GAGAAGGCCC ACAGCCACGG TGCTTCAAGG
    AAAGAACACC CCCAGTTTTT TCCTTCTAGT CCTCCACCCC ATTCCCCATT GAGTCACGGG
    CATATCCCCA GTGCCATCGT TCTTCCAAAC GCCACTCACG ACTACAATAC ATCCTTCTCC
    AACTCGAATG CCCACAAAGC CGAAAAGAAG CTTCAGAGCA TGGATCACCC TCTTACGAAG
    TCATCCAGTA AGCGGGAGCA CCGGCGGTCT GTGGATTCCA GGAATACTCT CAATGATCTC
    CTGAAGCATC TAAATGACCC AAACAGTAAC CCCAAAGCCA TCCTGGGAGA GATCCATATG
    GCTCATCAAA CCCTCATGCT GGACCCGGTG GGACCAATGG CTGAGGTCCC ACCCAAGGTC
    CCTAACCGGG AGGCATCTCT ATACTCCCCT CCCTCCACAC TCCCCAGAAA TAGTCCAACC
    AAGAGAGTAG ATGTCCCCAC CACTCCTGGG GTGCCAATGA CTTCTCTGGA AAGACAAAGG
    GGTTATCACA AAAATTCCTC CCAGAGGCAC TCTATATCTG CCGTGCCTAA AAACTTAAAC
    TCACCAAATG GTGTTTTGTT ATCTAGACAG CCGAGTATGA ACCGTGGAGG CTATATGCCC
    ACCCCAACAG GGGCGAAGGT GGACTATATT CAGGGGACAC CGGTGAGTGT TCATCTGCAG
    CCCTCCCTCT CCAGACAGAG CAGCTATACC AGTAATGGCA CCCTCCCCAG GACGGGACTA
    AAGAGGACAC CATCCTTAAA ACCTGATGTG CCACCAAAGC CTTCCTTTGT TCCGCAAACC
    ACATCTGTCA GACCACTGAA CAAGTACACG TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20490
    Clone ID Related Accession (Same CDS sequence) XM_030249343.1 , XM_030249345.1 , XM_017317001.1 , XM_017317001.2 , NM_199241.3
    Accession Version XM_030249343.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG
    GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC
    TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA
    GGTATCCCAA AGGGTGTACG GTGGGAAGTC CAGTCTGGAG AATCCAATCA GATGGTCCAC
    ATGAATGTCC TCATCACCTG CGTGTTTGCC GCTTTTGTCT TGGGCGCGTT CATCGCAGGA
    GTGGCCGTGT ACTGCTACCG TGACATGTTC GTTCGGAAGA ACAGAAAGAT CCATAAAGAC
    GCAGAATCCG CCCAGTCGTG CACAGACTCC AGCGGAAGCT TCGCCAAGCT GAACGGCCTC
    TTTGACAGCC CCGTCAAGGA ATACCAGCAG AACATTGATT CTCCCAAACT CTACAGCAAC
    CTGCTGACCA GTCGGAAGGA ACTGCCACCA AACACGGATA CAAAGTCCAT GGCCGTGGAC
    CACAGAGGCC AGCCTCCCGA GCTGGCTGCT CTCCCCACGC CGGAATCCAC ACCTGTCCTC
    CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCTGAGA AGGCCCACAG CCACGGTGCT
    TCAAGGAAAG AACACCCCCA GTTTTTTCCT TCTAGTCCTC CACCCCATTC CCCATTGAGT
    CACGGGCATA TCCCCAGTGC CATCGTTCTT CCAAACGCCA CTCACGACTA CAATACATCC
    TTCTCCAACT CGAATGCCCA CAAAGCCGAA AAGAAGCTTC AGAGCATGGA TCACCCTCTT
    ACGAAGTCAT CCAGTAAGCG GGAGCACCGG CGGTCTGTGG ATTCCAGGAA TACTCTCAAT
    GATCTCCTGA AGCATCTAAA TGACCCAAAC AGTAACCCCA AAGCCATCCT GGGAGAGATC
    CATATGGCTC ATCAAACCCT CATGCTGGAC CCGGTGGGAC CAATGGCTGA GGTCCCACCC
    AAGGTCCCTA ACCGGGAGGC ATCTCTATAC TCCCCTCCCT CCACACTCCC CAGAAATAGT
    CCAACCAAGA GAGTAGATGT CCCCACCACT CCTGGGGTGC CAATGACTTC TCTGGAAAGA
    CAAAGGGGTT ATCACAAAAA TTCCTCCCAG AGGCACTCTA TATCTGCCGT GCCTAAAAAC
    TTAAACTCAC CAAATGGTGT TTTGTTATCT AGACAGCCGA GTATGAACCG TGGAGGCTAT
    ATGCCCACCC CAACAGGGGC GAAGGTGGAC TATATTCAGG GGACACCGGT GAGTGTTCAT
    CTGCAGCCCT CCCTCTCCAG ACAGAGCAGC TATACCAGTA ATGGCACCCT CCCCAGGACG
    GGACTAAAGA GGACACCATC CTTAAAACCT GATGTGCCAC CAAAGCCTTC CTTTGTTCCG
    CAAACCACAT CTGTCAGACC ACTGAACAAG TACACGTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030105203.1
    CDS8748..11969
    Translation

    Target ORF information:

    RefSeq Version XM_030249343.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249343.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG
    GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC
    TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA
    GGTATCCCAA AGGGTGTACG GTGGGAAGTC CAGTCTGGAG AATCCAATCA GATGGTCCAC
    ATGAATGTCC TCATCACCTG CGTGTTTGCC GCTTTTGTCT TGGGCGCGTT CATCGCAGGA
    GTGGCCGTGT ACTGCTACCG TGACATGTTC GTTCGGAAGA ACAGAAAGAT CCATAAAGAC
    GCAGAATCCG CCCAGTCGTG CACAGACTCC AGCGGAAGCT TCGCCAAGCT GAACGGCCTC
    TTTGACAGCC CCGTCAAGGA ATACCAGCAG AACATTGATT CTCCCAAACT CTACAGCAAC
    CTGCTGACCA GTCGGAAGGA ACTGCCACCA AACACGGATA CAAAGTCCAT GGCCGTGGAC
    CACAGAGGCC AGCCTCCCGA GCTGGCTGCT CTCCCCACGC CGGAATCCAC ACCTGTCCTC
    CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCTGAGA AGGCCCACAG CCACGGTGCT
    TCAAGGAAAG AACACCCCCA GTTTTTTCCT TCTAGTCCTC CACCCCATTC CCCATTGAGT
    CACGGGCATA TCCCCAGTGC CATCGTTCTT CCAAACGCCA CTCACGACTA CAATACATCC
    TTCTCCAACT CGAATGCCCA CAAAGCCGAA AAGAAGCTTC AGAGCATGGA TCACCCTCTT
    ACGAAGTCAT CCAGTAAGCG GGAGCACCGG CGGTCTGTGG ATTCCAGGAA TACTCTCAAT
    GATCTCCTGA AGCATCTAAA TGACCCAAAC AGTAACCCCA AAGCCATCCT GGGAGAGATC
    CATATGGCTC ATCAAACCCT CATGCTGGAC CCGGTGGGAC CAATGGCTGA GGTCCCACCC
    AAGGTCCCTA ACCGGGAGGC ATCTCTATAC TCCCCTCCCT CCACACTCCC CAGAAATAGT
    CCAACCAAGA GAGTAGATGT CCCCACCACT CCTGGGGTGC CAATGACTTC TCTGGAAAGA
    CAAAGGGGTT ATCACAAAAA TTCCTCCCAG AGGCACTCTA TATCTGCCGT GCCTAAAAAC
    TTAAACTCAC CAAATGGTGT TTTGTTATCT AGACAGCCGA GTATGAACCG TGGAGGCTAT
    ATGCCCACCC CAACAGGGGC GAAGGTGGAC TATATTCAGG GGACACCGGT GAGTGTTCAT
    CTGCAGCCCT CCCTCTCCAG ACAGAGCAGC TATACCAGTA ATGGCACCCT CCCCAGGACG
    GGACTAAAGA GGACACCATC CTTAAAACCT GATGTGCCAC CAAAGCCTTC CTTTGTTCCG
    CAAACCACAT CTGTCAGACC ACTGAACAAG TACACGTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20490
    Clone ID Related Accession (Same CDS sequence) XM_030249343.1 , XM_030249345.1 , XM_017317001.1 , XM_017317001.2 , NM_199241.3
    Accession Version XM_030249345.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG
    GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC
    TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA
    GGTATCCCAA AGGGTGTACG GTGGGAAGTC CAGTCTGGAG AATCCAATCA GATGGTCCAC
    ATGAATGTCC TCATCACCTG CGTGTTTGCC GCTTTTGTCT TGGGCGCGTT CATCGCAGGA
    GTGGCCGTGT ACTGCTACCG TGACATGTTC GTTCGGAAGA ACAGAAAGAT CCATAAAGAC
    GCAGAATCCG CCCAGTCGTG CACAGACTCC AGCGGAAGCT TCGCCAAGCT GAACGGCCTC
    TTTGACAGCC CCGTCAAGGA ATACCAGCAG AACATTGATT CTCCCAAACT CTACAGCAAC
    CTGCTGACCA GTCGGAAGGA ACTGCCACCA AACACGGATA CAAAGTCCAT GGCCGTGGAC
    CACAGAGGCC AGCCTCCCGA GCTGGCTGCT CTCCCCACGC CGGAATCCAC ACCTGTCCTC
    CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCTGAGA AGGCCCACAG CCACGGTGCT
    TCAAGGAAAG AACACCCCCA GTTTTTTCCT TCTAGTCCTC CACCCCATTC CCCATTGAGT
    CACGGGCATA TCCCCAGTGC CATCGTTCTT CCAAACGCCA CTCACGACTA CAATACATCC
    TTCTCCAACT CGAATGCCCA CAAAGCCGAA AAGAAGCTTC AGAGCATGGA TCACCCTCTT
    ACGAAGTCAT CCAGTAAGCG GGAGCACCGG CGGTCTGTGG ATTCCAGGAA TACTCTCAAT
    GATCTCCTGA AGCATCTAAA TGACCCAAAC AGTAACCCCA AAGCCATCCT GGGAGAGATC
    CATATGGCTC ATCAAACCCT CATGCTGGAC CCGGTGGGAC CAATGGCTGA GGTCCCACCC
    AAGGTCCCTA ACCGGGAGGC ATCTCTATAC TCCCCTCCCT CCACACTCCC CAGAAATAGT
    CCAACCAAGA GAGTAGATGT CCCCACCACT CCTGGGGTGC CAATGACTTC TCTGGAAAGA
    CAAAGGGGTT ATCACAAAAA TTCCTCCCAG AGGCACTCTA TATCTGCCGT GCCTAAAAAC
    TTAAACTCAC CAAATGGTGT TTTGTTATCT AGACAGCCGA GTATGAACCG TGGAGGCTAT
    ATGCCCACCC CAACAGGGGC GAAGGTGGAC TATATTCAGG GGACACCGGT GAGTGTTCAT
    CTGCAGCCCT CCCTCTCCAG ACAGAGCAGC TATACCAGTA ATGGCACCCT CCCCAGGACG
    GGACTAAAGA GGACACCATC CTTAAAACCT GATGTGCCAC CAAAGCCTTC CTTTGTTCCG
    CAAACCACAT CTGTCAGACC ACTGAACAAG TACACGTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030105205.1
    CDS832..4053
    Translation

    Target ORF information:

    RefSeq Version XM_030249345.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249345.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG
    GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC
    TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA
    GGTATCCCAA AGGGTGTACG GTGGGAAGTC CAGTCTGGAG AATCCAATCA GATGGTCCAC
    ATGAATGTCC TCATCACCTG CGTGTTTGCC GCTTTTGTCT TGGGCGCGTT CATCGCAGGA
    GTGGCCGTGT ACTGCTACCG TGACATGTTC GTTCGGAAGA ACAGAAAGAT CCATAAAGAC
    GCAGAATCCG CCCAGTCGTG CACAGACTCC AGCGGAAGCT TCGCCAAGCT GAACGGCCTC
    TTTGACAGCC CCGTCAAGGA ATACCAGCAG AACATTGATT CTCCCAAACT CTACAGCAAC
    CTGCTGACCA GTCGGAAGGA ACTGCCACCA AACACGGATA CAAAGTCCAT GGCCGTGGAC
    CACAGAGGCC AGCCTCCCGA GCTGGCTGCT CTCCCCACGC CGGAATCCAC ACCTGTCCTC
    CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCTGAGA AGGCCCACAG CCACGGTGCT
    TCAAGGAAAG AACACCCCCA GTTTTTTCCT TCTAGTCCTC CACCCCATTC CCCATTGAGT
    CACGGGCATA TCCCCAGTGC CATCGTTCTT CCAAACGCCA CTCACGACTA CAATACATCC
    TTCTCCAACT CGAATGCCCA CAAAGCCGAA AAGAAGCTTC AGAGCATGGA TCACCCTCTT
    ACGAAGTCAT CCAGTAAGCG GGAGCACCGG CGGTCTGTGG ATTCCAGGAA TACTCTCAAT
    GATCTCCTGA AGCATCTAAA TGACCCAAAC AGTAACCCCA AAGCCATCCT GGGAGAGATC
    CATATGGCTC ATCAAACCCT CATGCTGGAC CCGGTGGGAC CAATGGCTGA GGTCCCACCC
    AAGGTCCCTA ACCGGGAGGC ATCTCTATAC TCCCCTCCCT CCACACTCCC CAGAAATAGT
    CCAACCAAGA GAGTAGATGT CCCCACCACT CCTGGGGTGC CAATGACTTC TCTGGAAAGA
    CAAAGGGGTT ATCACAAAAA TTCCTCCCAG AGGCACTCTA TATCTGCCGT GCCTAAAAAC
    TTAAACTCAC CAAATGGTGT TTTGTTATCT AGACAGCCGA GTATGAACCG TGGAGGCTAT
    ATGCCCACCC CAACAGGGGC GAAGGTGGAC TATATTCAGG GGACACCGGT GAGTGTTCAT
    CTGCAGCCCT CCCTCTCCAG ACAGAGCAGC TATACCAGTA ATGGCACCCT CCCCAGGACG
    GGACTAAAGA GGACACCATC CTTAAAACCT GATGTGCCAC CAAAGCCTTC CTTTGTTCCG
    CAAACCACAT CTGTCAGACC ACTGAACAAG TACACGTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20490
    Clone ID Related Accession (Same CDS sequence) XM_030249343.1 , XM_030249345.1 , XM_017317001.1 , XM_017317001.2 , NM_199241.3
    Accession Version XM_017317001.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317001.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG
    GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC
    TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA
    GGTATCCCAA AGGGTGTACG GTGGGAAGTC CAGTCTGGAG AATCCAATCA GATGGTCCAC
    ATGAATGTCC TCATCACCTG CGTGTTTGCC GCTTTTGTCT TGGGCGCGTT CATCGCAGGA
    GTGGCCGTGT ACTGCTACCG TGACATGTTC GTTCGGAAGA ACAGAAAGAT CCATAAAGAC
    GCAGAATCCG CCCAGTCGTG CACAGACTCC AGCGGAAGCT TCGCCAAGCT GAACGGCCTC
    TTTGACAGCC CCGTCAAGGA ATACCAGCAG AACATTGATT CTCCCAAACT CTACAGCAAC
    CTGCTGACCA GTCGGAAGGA ACTGCCACCA AACACGGATA CAAAGTCCAT GGCCGTGGAC
    CACAGAGGCC AGCCTCCCGA GCTGGCTGCT CTCCCCACGC CGGAATCCAC ACCTGTCCTC
    CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCTGAGA AGGCCCACAG CCACGGTGCT
    TCAAGGAAAG AACACCCCCA GTTTTTTCCT TCTAGTCCTC CACCCCATTC CCCATTGAGT
    CACGGGCATA TCCCCAGTGC CATCGTTCTT CCAAACGCCA CTCACGACTA CAATACATCC
    TTCTCCAACT CGAATGCCCA CAAAGCCGAA AAGAAGCTTC AGAGCATGGA TCACCCTCTT
    ACGAAGTCAT CCAGTAAGCG GGAGCACCGG CGGTCTGTGG ATTCCAGGAA TACTCTCAAT
    GATCTCCTGA AGCATCTAAA TGACCCAAAC AGTAACCCCA AAGCCATCCT GGGAGAGATC
    CATATGGCTC ATCAAACCCT CATGCTGGAC CCGGTGGGAC CAATGGCTGA GGTCCCACCC
    AAGGTCCCTA ACCGGGAGGC ATCTCTATAC TCCCCTCCCT CCACACTCCC CAGAAATAGT
    CCAACCAAGA GAGTAGATGT CCCCACCACT CCTGGGGTGC CAATGACTTC TCTGGAAAGA
    CAAAGGGGTT ATCACAAAAA TTCCTCCCAG AGGCACTCTA TATCTGCCGT GCCTAAAAAC
    TTAAACTCAC CAAATGGTGT TTTGTTATCT AGACAGCCGA GTATGAACCG TGGAGGCTAT
    ATGCCCACCC CAACAGGGGC GAAGGTGGAC TATATTCAGG GGACACCGGT GAGTGTTCAT
    CTGCAGCCCT CCCTCTCCAG ACAGAGCAGC TATACCAGTA ATGGCACCCT CCCCAGGACG
    GGACTAAAGA GGACACCATC CTTAAAACCT GATGTGCCAC CAAAGCCTTC CTTTGTTCCG
    CAAACCACAT CTGTCAGACC ACTGAACAAG TACACGTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172490.1
    CDS830..4051
    Translation

    Target ORF information:

    RefSeq Version XM_017317001.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317001.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG
    GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC
    TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA
    GGTATCCCAA AGGGTGTACG GTGGGAAGTC CAGTCTGGAG AATCCAATCA GATGGTCCAC
    ATGAATGTCC TCATCACCTG CGTGTTTGCC GCTTTTGTCT TGGGCGCGTT CATCGCAGGA
    GTGGCCGTGT ACTGCTACCG TGACATGTTC GTTCGGAAGA ACAGAAAGAT CCATAAAGAC
    GCAGAATCCG CCCAGTCGTG CACAGACTCC AGCGGAAGCT TCGCCAAGCT GAACGGCCTC
    TTTGACAGCC CCGTCAAGGA ATACCAGCAG AACATTGATT CTCCCAAACT CTACAGCAAC
    CTGCTGACCA GTCGGAAGGA ACTGCCACCA AACACGGATA CAAAGTCCAT GGCCGTGGAC
    CACAGAGGCC AGCCTCCCGA GCTGGCTGCT CTCCCCACGC CGGAATCCAC ACCTGTCCTC
    CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCTGAGA AGGCCCACAG CCACGGTGCT
    TCAAGGAAAG AACACCCCCA GTTTTTTCCT TCTAGTCCTC CACCCCATTC CCCATTGAGT
    CACGGGCATA TCCCCAGTGC CATCGTTCTT CCAAACGCCA CTCACGACTA CAATACATCC
    TTCTCCAACT CGAATGCCCA CAAAGCCGAA AAGAAGCTTC AGAGCATGGA TCACCCTCTT
    ACGAAGTCAT CCAGTAAGCG GGAGCACCGG CGGTCTGTGG ATTCCAGGAA TACTCTCAAT
    GATCTCCTGA AGCATCTAAA TGACCCAAAC AGTAACCCCA AAGCCATCCT GGGAGAGATC
    CATATGGCTC ATCAAACCCT CATGCTGGAC CCGGTGGGAC CAATGGCTGA GGTCCCACCC
    AAGGTCCCTA ACCGGGAGGC ATCTCTATAC TCCCCTCCCT CCACACTCCC CAGAAATAGT
    CCAACCAAGA GAGTAGATGT CCCCACCACT CCTGGGGTGC CAATGACTTC TCTGGAAAGA
    CAAAGGGGTT ATCACAAAAA TTCCTCCCAG AGGCACTCTA TATCTGCCGT GCCTAAAAAC
    TTAAACTCAC CAAATGGTGT TTTGTTATCT AGACAGCCGA GTATGAACCG TGGAGGCTAT
    ATGCCCACCC CAACAGGGGC GAAGGTGGAC TATATTCAGG GGACACCGGT GAGTGTTCAT
    CTGCAGCCCT CCCTCTCCAG ACAGAGCAGC TATACCAGTA ATGGCACCCT CCCCAGGACG
    GGACTAAAGA GGACACCATC CTTAAAACCT GATGTGCCAC CAAAGCCTTC CTTTGTTCCG
    CAAACCACAT CTGTCAGACC ACTGAACAAG TACACGTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20528
    Clone ID Related Accession (Same CDS sequence) XM_017317012.2 , XM_030249363.1 , XM_011239427.3 , XM_030249361.1 , XM_017317012.1 , XM_011239427.2 , NM_199240.3
    Accession Version XM_017317012.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3165bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317012.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT
    GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA
    TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC
    CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA
    GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA
    GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC
    CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC
    AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG
    GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC
    CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT
    GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG
    AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA
    TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT
    CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC
    AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG
    ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA
    CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT
    AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA
    AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA
    AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC
    TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT
    CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG
    ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT
    CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172501.1
    CDS830..3994
    Translation

    Target ORF information:

    RefSeq Version XM_017317012.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317012.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT
    GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA
    TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC
    CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA
    GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA
    GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC
    CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC
    AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG
    GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC
    CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT
    GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG
    AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA
    TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT
    CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC
    AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG
    ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA
    CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT
    AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA
    AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA
    AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC
    TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT
    CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG
    ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT
    CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20528
    Clone ID Related Accession (Same CDS sequence) XM_017317012.2 , XM_030249363.1 , XM_011239427.3 , XM_030249361.1 , XM_017317012.1 , XM_011239427.2 , NM_199240.3
    Accession Version XM_030249363.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3165bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT
    GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA
    TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC
    CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA
    GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA
    GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC
    CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC
    AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG
    GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC
    CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT
    GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG
    AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA
    TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT
    CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC
    AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG
    ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA
    CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT
    AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA
    AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA
    AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC
    TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT
    CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG
    ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT
    CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030105223.1
    CDS394..3558
    Translation

    Target ORF information:

    RefSeq Version XM_030249363.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249363.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT
    GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA
    TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC
    CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA
    GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA
    GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC
    CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC
    AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG
    GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC
    CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT
    GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG
    AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA
    TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT
    CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC
    AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG
    ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA
    CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT
    AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA
    AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA
    AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC
    TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT
    CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG
    ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT
    CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20528
    Clone ID Related Accession (Same CDS sequence) XM_017317012.2 , XM_030249363.1 , XM_011239427.3 , XM_030249361.1 , XM_017317012.1 , XM_011239427.2 , NM_199240.3
    Accession Version XM_011239427.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3165bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011239427.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT
    GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA
    TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC
    CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA
    GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA
    GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC
    CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC
    AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG
    GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC
    CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT
    GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG
    AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA
    TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT
    CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC
    AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG
    ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA
    CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT
    AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA
    AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA
    AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC
    TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT
    CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG
    ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT
    CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011237729.1
    CDS605..3769
    Translation

    Target ORF information:

    RefSeq Version XM_011239427.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011239427.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT
    GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA
    TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC
    CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA
    GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA
    GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC
    CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC
    AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG
    GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC
    CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT
    GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG
    AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA
    TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT
    CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC
    AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG
    ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA
    CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT
    AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA
    AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA
    AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC
    TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT
    CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG
    ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT
    CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20528
    Clone ID Related Accession (Same CDS sequence) XM_017317012.2 , XM_030249363.1 , XM_011239427.3 , XM_030249361.1 , XM_017317012.1 , XM_011239427.2 , NM_199240.3
    Accession Version XM_030249361.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3165bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT
    GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA
    TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC
    CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA
    GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA
    GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC
    CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC
    AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG
    GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC
    CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT
    GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG
    AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA
    TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT
    CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC
    AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG
    ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA
    CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT
    AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA
    AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA
    AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC
    TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT
    CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG
    ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT
    CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030105221.1
    CDS8748..11912
    Translation

    Target ORF information:

    RefSeq Version XM_030249361.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249361.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT
    GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA
    TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC
    CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA
    GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA
    GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC
    CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC
    AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG
    GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC
    CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT
    GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG
    AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA
    TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT
    CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC
    AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG
    ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA
    CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT
    AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA
    AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA
    AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC
    TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT
    CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG
    ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT
    CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20556
    Clone ID Related Accession (Same CDS sequence) NM_001291000.1 , XM_030249347.1 , XM_030249351.1
    Accession Version XM_030249347.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG
    GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC
    TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA
    GGTGTACGGT GGGAAGTCCA GTCTGGAGAA TCCAATCAGA TGGTCCACAT GAATGTCCTC
    ATCACCTGCG TGTTTGCCGC TTTTGTCTTG GGCGCGTTCA TCGCAGGAGT GGCCGTGTAC
    TGCTACCGTG ACATGTTCGT TCGGAAGAAC AGAAAGATCC ATAAAGACGC AGAATCCGCC
    CAGTCGTGCA CAGACTCCAG CGGAAGCTTC GCCAAGCTGA ACGGCCTCTT TGACAGCCCC
    GTCAAGGAAT ACCAGCAGAA CATTGATTCT CCCAAACTCT ACAGCAACCT GCTGACCAGT
    CGGAAGGAAC TGCCACCAAA CACGGATACA AAGTCCATGG CCGTGGACCA CAGAGGCCAG
    CCTCCCGAGC TGGCTGCTCT CCCCACGCCG GAATCCACAC CTGTCCTCCA CCAGAAGACC
    CTGCAGGCCA TGAAGAGCCA CTCTGAGAAG GCCCACAGCC ACGGTGCTTC AAGGAAAGAA
    CACCCCCAGT TTTTTCCTTC TAGTCCTCCA CCCCATTCCC CATTGAGTCA CGGGCATATC
    CCCAGTGCCA TCGTTCTTCC AAACGCCACT CACGACTACA ATACATCCTT CTCCAACTCG
    AATGCCCACA AAGCCGAAAA GAAGCTTCAG AGCATGGATC ACCCTCTTAC GAAGTCATCC
    AGTAAGCGGG AGCACCGGCG GTCTGTGGAT TCCAGGAATA CTCTCAATGA TCTCCTGAAG
    CATCTAAATG ACCCAAACAG TAACCCCAAA GCCATCCTGG GAGAGATCCA TATGGCTCAT
    CAAACCCTCA TGCTGGACCC GGTGGGACCA ATGGCTGAGG TCCCACCCAA GGTCCCTAAC
    CGGGAGGCAT CTCTATACTC CCCTCCCTCC ACACTCCCCA GAAATAGTCC AACCAAGAGA
    GTAGATGTCC CCACCACTCC TGGGGTGCCA ATGACTTCTC TGGAAAGACA AAGGGGTTAT
    CACAAAAATT CCTCCCAGAG GCACTCTATA TCTGCCGTGC CTAAAAACTT AAACTCACCA
    AATGGTGTTT TGTTATCTAG ACAGCCGAGT ATGAACCGTG GAGGCTATAT GCCCACCCCA
    ACAGGGGCGA AGGTGGACTA TATTCAGGGG ACACCGGTGA GTGTTCATCT GCAGCCCTCC
    CTCTCCAGAC AGAGCAGCTA TACCAGTAAT GGCACCCTCC CCAGGACGGG ACTAAAGAGG
    ACACCATCCT TAAAACCTGA TGTGCCACCA AAGCCTTCCT TTGTTCCGCA AACCACATCT
    GTCAGACCAC TGAACAAGTA CACGTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030105207.1
    CDS8748..11957
    Translation

    Target ORF information:

    RefSeq Version XM_030249347.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249347.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG
    GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC
    TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA
    GGTGTACGGT GGGAAGTCCA GTCTGGAGAA TCCAATCAGA TGGTCCACAT GAATGTCCTC
    ATCACCTGCG TGTTTGCCGC TTTTGTCTTG GGCGCGTTCA TCGCAGGAGT GGCCGTGTAC
    TGCTACCGTG ACATGTTCGT TCGGAAGAAC AGAAAGATCC ATAAAGACGC AGAATCCGCC
    CAGTCGTGCA CAGACTCCAG CGGAAGCTTC GCCAAGCTGA ACGGCCTCTT TGACAGCCCC
    GTCAAGGAAT ACCAGCAGAA CATTGATTCT CCCAAACTCT ACAGCAACCT GCTGACCAGT
    CGGAAGGAAC TGCCACCAAA CACGGATACA AAGTCCATGG CCGTGGACCA CAGAGGCCAG
    CCTCCCGAGC TGGCTGCTCT CCCCACGCCG GAATCCACAC CTGTCCTCCA CCAGAAGACC
    CTGCAGGCCA TGAAGAGCCA CTCTGAGAAG GCCCACAGCC ACGGTGCTTC AAGGAAAGAA
    CACCCCCAGT TTTTTCCTTC TAGTCCTCCA CCCCATTCCC CATTGAGTCA CGGGCATATC
    CCCAGTGCCA TCGTTCTTCC AAACGCCACT CACGACTACA ATACATCCTT CTCCAACTCG
    AATGCCCACA AAGCCGAAAA GAAGCTTCAG AGCATGGATC ACCCTCTTAC GAAGTCATCC
    AGTAAGCGGG AGCACCGGCG GTCTGTGGAT TCCAGGAATA CTCTCAATGA TCTCCTGAAG
    CATCTAAATG ACCCAAACAG TAACCCCAAA GCCATCCTGG GAGAGATCCA TATGGCTCAT
    CAAACCCTCA TGCTGGACCC GGTGGGACCA ATGGCTGAGG TCCCACCCAA GGTCCCTAAC
    CGGGAGGCAT CTCTATACTC CCCTCCCTCC ACACTCCCCA GAAATAGTCC AACCAAGAGA
    GTAGATGTCC CCACCACTCC TGGGGTGCCA ATGACTTCTC TGGAAAGACA AAGGGGTTAT
    CACAAAAATT CCTCCCAGAG GCACTCTATA TCTGCCGTGC CTAAAAACTT AAACTCACCA
    AATGGTGTTT TGTTATCTAG ACAGCCGAGT ATGAACCGTG GAGGCTATAT GCCCACCCCA
    ACAGGGGCGA AGGTGGACTA TATTCAGGGG ACACCGGTGA GTGTTCATCT GCAGCCCTCC
    CTCTCCAGAC AGAGCAGCTA TACCAGTAAT GGCACCCTCC CCAGGACGGG ACTAAAGAGG
    ACACCATCCT TAAAACCTGA TGTGCCACCA AAGCCTTCCT TTGTTCCGCA AACCACATCT
    GTCAGACCAC TGAACAAGTA CACGTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20556
    Clone ID Related Accession (Same CDS sequence) NM_001291000.1 , XM_030249347.1 , XM_030249351.1
    Accession Version XM_030249351.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG
    GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC
    TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA
    GGTGTACGGT GGGAAGTCCA GTCTGGAGAA TCCAATCAGA TGGTCCACAT GAATGTCCTC
    ATCACCTGCG TGTTTGCCGC TTTTGTCTTG GGCGCGTTCA TCGCAGGAGT GGCCGTGTAC
    TGCTACCGTG ACATGTTCGT TCGGAAGAAC AGAAAGATCC ATAAAGACGC AGAATCCGCC
    CAGTCGTGCA CAGACTCCAG CGGAAGCTTC GCCAAGCTGA ACGGCCTCTT TGACAGCCCC
    GTCAAGGAAT ACCAGCAGAA CATTGATTCT CCCAAACTCT ACAGCAACCT GCTGACCAGT
    CGGAAGGAAC TGCCACCAAA CACGGATACA AAGTCCATGG CCGTGGACCA CAGAGGCCAG
    CCTCCCGAGC TGGCTGCTCT CCCCACGCCG GAATCCACAC CTGTCCTCCA CCAGAAGACC
    CTGCAGGCCA TGAAGAGCCA CTCTGAGAAG GCCCACAGCC ACGGTGCTTC AAGGAAAGAA
    CACCCCCAGT TTTTTCCTTC TAGTCCTCCA CCCCATTCCC CATTGAGTCA CGGGCATATC
    CCCAGTGCCA TCGTTCTTCC AAACGCCACT CACGACTACA ATACATCCTT CTCCAACTCG
    AATGCCCACA AAGCCGAAAA GAAGCTTCAG AGCATGGATC ACCCTCTTAC GAAGTCATCC
    AGTAAGCGGG AGCACCGGCG GTCTGTGGAT TCCAGGAATA CTCTCAATGA TCTCCTGAAG
    CATCTAAATG ACCCAAACAG TAACCCCAAA GCCATCCTGG GAGAGATCCA TATGGCTCAT
    CAAACCCTCA TGCTGGACCC GGTGGGACCA ATGGCTGAGG TCCCACCCAA GGTCCCTAAC
    CGGGAGGCAT CTCTATACTC CCCTCCCTCC ACACTCCCCA GAAATAGTCC AACCAAGAGA
    GTAGATGTCC CCACCACTCC TGGGGTGCCA ATGACTTCTC TGGAAAGACA AAGGGGTTAT
    CACAAAAATT CCTCCCAGAG GCACTCTATA TCTGCCGTGC CTAAAAACTT AAACTCACCA
    AATGGTGTTT TGTTATCTAG ACAGCCGAGT ATGAACCGTG GAGGCTATAT GCCCACCCCA
    ACAGGGGCGA AGGTGGACTA TATTCAGGGG ACACCGGTGA GTGTTCATCT GCAGCCCTCC
    CTCTCCAGAC AGAGCAGCTA TACCAGTAAT GGCACCCTCC CCAGGACGGG ACTAAAGAGG
    ACACCATCCT TAAAACCTGA TGTGCCACCA AAGCCTTCCT TTGTTCCGCA AACCACATCT
    GTCAGACCAC TGAACAAGTA CACGTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030105211.1
    CDS832..4041
    Translation

    Target ORF information:

    RefSeq Version XM_030249351.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249351.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG
    GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC
    TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA
    GGTGTACGGT GGGAAGTCCA GTCTGGAGAA TCCAATCAGA TGGTCCACAT GAATGTCCTC
    ATCACCTGCG TGTTTGCCGC TTTTGTCTTG GGCGCGTTCA TCGCAGGAGT GGCCGTGTAC
    TGCTACCGTG ACATGTTCGT TCGGAAGAAC AGAAAGATCC ATAAAGACGC AGAATCCGCC
    CAGTCGTGCA CAGACTCCAG CGGAAGCTTC GCCAAGCTGA ACGGCCTCTT TGACAGCCCC
    GTCAAGGAAT ACCAGCAGAA CATTGATTCT CCCAAACTCT ACAGCAACCT GCTGACCAGT
    CGGAAGGAAC TGCCACCAAA CACGGATACA AAGTCCATGG CCGTGGACCA CAGAGGCCAG
    CCTCCCGAGC TGGCTGCTCT CCCCACGCCG GAATCCACAC CTGTCCTCCA CCAGAAGACC
    CTGCAGGCCA TGAAGAGCCA CTCTGAGAAG GCCCACAGCC ACGGTGCTTC AAGGAAAGAA
    CACCCCCAGT TTTTTCCTTC TAGTCCTCCA CCCCATTCCC CATTGAGTCA CGGGCATATC
    CCCAGTGCCA TCGTTCTTCC AAACGCCACT CACGACTACA ATACATCCTT CTCCAACTCG
    AATGCCCACA AAGCCGAAAA GAAGCTTCAG AGCATGGATC ACCCTCTTAC GAAGTCATCC
    AGTAAGCGGG AGCACCGGCG GTCTGTGGAT TCCAGGAATA CTCTCAATGA TCTCCTGAAG
    CATCTAAATG ACCCAAACAG TAACCCCAAA GCCATCCTGG GAGAGATCCA TATGGCTCAT
    CAAACCCTCA TGCTGGACCC GGTGGGACCA ATGGCTGAGG TCCCACCCAA GGTCCCTAAC
    CGGGAGGCAT CTCTATACTC CCCTCCCTCC ACACTCCCCA GAAATAGTCC AACCAAGAGA
    GTAGATGTCC CCACCACTCC TGGGGTGCCA ATGACTTCTC TGGAAAGACA AAGGGGTTAT
    CACAAAAATT CCTCCCAGAG GCACTCTATA TCTGCCGTGC CTAAAAACTT AAACTCACCA
    AATGGTGTTT TGTTATCTAG ACAGCCGAGT ATGAACCGTG GAGGCTATAT GCCCACCCCA
    ACAGGGGCGA AGGTGGACTA TATTCAGGGG ACACCGGTGA GTGTTCATCT GCAGCCCTCC
    CTCTCCAGAC AGAGCAGCTA TACCAGTAAT GGCACCCTCC CCAGGACGGG ACTAAAGAGG
    ACACCATCCT TAAAACCTGA TGTGCCACCA AAGCCTTCCT TTGTTCCGCA AACCACATCT
    GTCAGACCAC TGAACAAGTA CACGTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu30226
    Clone ID Related Accession (Same CDS sequence) XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
    Accession Version XM_030249335.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030105195.1
    CDS471..3731
    Translation

    Target ORF information:

    RefSeq Version XM_030249335.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249335.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu30226
    Clone ID Related Accession (Same CDS sequence) XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
    Accession Version XM_006499111.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499111.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006499174.1
    CDS830..4090
    Translation

    Target ORF information:

    RefSeq Version XM_006499111.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006499111.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu30226
    Clone ID Related Accession (Same CDS sequence) XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
    Accession Version XM_006499112.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499112.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006499175.1
    CDS485..3745
    Translation

    Target ORF information:

    RefSeq Version XM_006499112.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006499112.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu30226
    Clone ID Related Accession (Same CDS sequence) XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
    Accession Version XM_030249334.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030105194.1
    CDS444..3704
    Translation

    Target ORF information:

    RefSeq Version XM_030249334.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249334.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu30226
    Clone ID Related Accession (Same CDS sequence) XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
    Accession Version XM_006499113.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499113.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006499176.1
    CDS8748..12008
    Translation

    Target ORF information:

    RefSeq Version XM_006499113.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006499113.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu30226
    Clone ID Related Accession (Same CDS sequence) XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
    Accession Version XM_006499114.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499114.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006499177.1
    CDS839..4099
    Translation

    Target ORF information:

    RefSeq Version XM_006499114.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006499114.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu72911
    Clone ID Related Accession (Same CDS sequence) XM_017316995.2 , XM_017316995.1
    Accession Version XM_017316995.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316995.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTGTACGGTG GGAAGTCCAG
    TCTGGAGAAT CCAATCAGAT GGTCCACATG AATGTCCTCA TCACCTGCGT GTTTGCCGCT
    TTTGTCTTGG GCGCGTTCAT CGCAGGAGTG GCCGTGTACT GCTACCGTGA CATGTTCGTT
    CGGAAGAACA GAAAGATCCA TAAAGACGCA GAATCCGCCC AGTCGTGCAC AGACTCCAGC
    GGAAGCTTCG CCAAGCTGAA CGGCCTCTTT GACAGCCCCG TCAAGGAATA CCAGCAGAAC
    ATTGATTCTC CCAAACTCTA CAGCAACCTG CTGACCAGTC GGAAGGAACT GCCACCAAAC
    ACGGATACAA AGTCCATGGC CGTGGACCAC AGAGGCCAGC CTCCCGAGCT GGCTGCTCTC
    CCCACGCCGG AATCCACACC TGTCCTCCAC CAGAAGACCC TGCAGGCCAT GAAGAGCCAC
    TCTGAGAAGG CCCACAGCCA CGGTGCTTCA AGGAAAGAAC ACCCCCAGTT TTTTCCTTCT
    AGTCCTCCAC CCCATTCCCC ATTGAGTCAC GGGCATATCC CCAGTGCCAT CGTTCTTCCA
    AACGCCACTC ACGACTACAA TACATCCTTC TCCAACTCGA ATGCCCACAA AGCCGAAAAG
    AAGCTTCAGA GCATGGATCA CCCTCTTACG AAGTCATCCA GTAAGCGGGA GCACCGGCGG
    TCTGTGGATT CCAGGAATAC TCTCAATGAT CTCCTGAAGC ATCTAAATGA CCCAAACAGT
    AACCCCAAAG CCATCCTGGG AGAGATCCAT ATGGCTCATC AAACCCTCAT GCTGGACCCG
    GTGGGACCAA TGGCTGAGGT CCCACCCAAG GTCCCTAACC GGGAGGCATC TCTATACTCC
    CCTCCCTCCA CACTCCCCAG AAATAGTCCA ACCAAGAGAG TAGATGTCCC CACCACTCCT
    GGGGTGCCAA TGACTTCTCT GGAAAGACAA AGGGGTTATC ACAAAAATTC CTCCCAGAGG
    CACTCTATAT CTGCCGTGCC TAAAAACTTA AACTCACCAA ATGGTGTTTT GTTATCTAGA
    CAGCCGAGTA TGAACCGTGG AGGCTATATG CCCACCCCAA CAGGGGCGAA GGTGGACTAT
    ATTCAGGGGA CACCGGTGAG TGTTCATCTG CAGCCCTCCC TCTCCAGACA GAGCAGCTAT
    ACCAGTAATG GCACCCTCCC CAGGACGGGA CTAAAGAGGA CACCATCCTT AAAACCTGAT
    GTGCCACCAA AGCCTTCCTT TGTTCCGCAA ACCACATCTG TCAGACCACT GAACAAGTAC
    ACGTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172484.1
    CDS830..4078
    Translation

    Target ORF information:

    RefSeq Version XM_017316995.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017316995.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTGTACGGTG GGAAGTCCAG
    TCTGGAGAAT CCAATCAGAT GGTCCACATG AATGTCCTCA TCACCTGCGT GTTTGCCGCT
    TTTGTCTTGG GCGCGTTCAT CGCAGGAGTG GCCGTGTACT GCTACCGTGA CATGTTCGTT
    CGGAAGAACA GAAAGATCCA TAAAGACGCA GAATCCGCCC AGTCGTGCAC AGACTCCAGC
    GGAAGCTTCG CCAAGCTGAA CGGCCTCTTT GACAGCCCCG TCAAGGAATA CCAGCAGAAC
    ATTGATTCTC CCAAACTCTA CAGCAACCTG CTGACCAGTC GGAAGGAACT GCCACCAAAC
    ACGGATACAA AGTCCATGGC CGTGGACCAC AGAGGCCAGC CTCCCGAGCT GGCTGCTCTC
    CCCACGCCGG AATCCACACC TGTCCTCCAC CAGAAGACCC TGCAGGCCAT GAAGAGCCAC
    TCTGAGAAGG CCCACAGCCA CGGTGCTTCA AGGAAAGAAC ACCCCCAGTT TTTTCCTTCT
    AGTCCTCCAC CCCATTCCCC ATTGAGTCAC GGGCATATCC CCAGTGCCAT CGTTCTTCCA
    AACGCCACTC ACGACTACAA TACATCCTTC TCCAACTCGA ATGCCCACAA AGCCGAAAAG
    AAGCTTCAGA GCATGGATCA CCCTCTTACG AAGTCATCCA GTAAGCGGGA GCACCGGCGG
    TCTGTGGATT CCAGGAATAC TCTCAATGAT CTCCTGAAGC ATCTAAATGA CCCAAACAGT
    AACCCCAAAG CCATCCTGGG AGAGATCCAT ATGGCTCATC AAACCCTCAT GCTGGACCCG
    GTGGGACCAA TGGCTGAGGT CCCACCCAAG GTCCCTAACC GGGAGGCATC TCTATACTCC
    CCTCCCTCCA CACTCCCCAG AAATAGTCCA ACCAAGAGAG TAGATGTCCC CACCACTCCT
    GGGGTGCCAA TGACTTCTCT GGAAAGACAA AGGGGTTATC ACAAAAATTC CTCCCAGAGG
    CACTCTATAT CTGCCGTGCC TAAAAACTTA AACTCACCAA ATGGTGTTTT GTTATCTAGA
    CAGCCGAGTA TGAACCGTGG AGGCTATATG CCCACCCCAA CAGGGGCGAA GGTGGACTAT
    ATTCAGGGGA CACCGGTGAG TGTTCATCTG CAGCCCTCCC TCTCCAGACA GAGCAGCTAT
    ACCAGTAATG GCACCCTCCC CAGGACGGGA CTAAAGAGGA CACCATCCTT AAAACCTGAT
    GTGCCACCAA AGCCTTCCTT TGTTCCGCAA ACCACATCTG TCAGACCACT GAACAAGTAC
    ACGTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu72912
    Clone ID Related Accession (Same CDS sequence) XM_030249357.1 , XM_017317010.1 , XM_017317010.2
    Accession Version XM_030249357.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3204bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGACA TGGAGGTATC CTCATCTTCT GTTACCACTG TGGCAAGTAG CCCAGAAATT
    ACATCTAAAG TGATTGATAC CTGGAGACCT AAACTGACGA GCTCCCGGAA ATTTGTAGTT
    CAAGATGACC CAAATACTTC TGATTTTACT GATACTATAT CAGGTATCCC AAAGGGTGTA
    CGGTGGGAAG TCCAGTCTGG AGAATCCAAT CAGATGGTCC ACATGAATGT CCTCATCACC
    TGCGTGTTTG CCGCTTTTGT CTTGGGCGCG TTCATCGCAG GAGTGGCCGT GTACTGCTAC
    CGTGACATGT TCGTTCGGAA GAACAGAAAG ATCCATAAAG ACGCAGAATC CGCCCAGTCG
    TGCACAGACT CCAGCGGAAG CTTCGCCAAG CTGAACGGCC TCTTTGACAG CCCCGTCAAG
    GAATACCAGC AGAACATTGA TTCTCCCAAA CTCTACAGCA ACCTGCTGAC CAGTCGGAAG
    GAACTGCCAC CAAACACGGA TACAAAGTCC ATGGCCGTGG ACCACAGAGG CCAGCCTCCC
    GAGCTGGCTG CTCTCCCCAC GCCGGAATCC ACACCTGTCC TCCACCAGAA GACCCTGCAG
    GCCATGAAGA GCCACTCTGA GAAGGCCCAC AGCCACGGTG CTTCAAGGAA AGAACACCCC
    CAGTTTTTTC CTTCTAGTCC TCCACCCCAT TCCCCATTGA GTCACGGGCA TATCCCCAGT
    GCCATCGTTC TTCCAAACGC CACTCACGAC TACAATACAT CCTTCTCCAA CTCGAATGCC
    CACAAAGCCG AAAAGAAGCT TCAGAGCATG GATCACCCTC TTACGAAGTC ATCCAGTAAG
    CGGGAGCACC GGCGGTCTGT GGATTCCAGG AATACTCTCA ATGATCTCCT GAAGCATCTA
    AATGACCCAA ACAGTAACCC CAAAGCCATC CTGGGAGAGA TCCATATGGC TCATCAAACC
    CTCATGCTGG ACCCGGTGGG ACCAATGGCT GAGGTCCCAC CCAAGGTCCC TAACCGGGAG
    GCATCTCTAT ACTCCCCTCC CTCCACACTC CCCAGAAATA GTCCAACCAA GAGAGTAGAT
    GTCCCCACCA CTCCTGGGGT GCCAATGACT TCTCTGGAAA GACAAAGGGG TTATCACAAA
    AATTCCTCCC AGAGGCACTC TATATCTGCC GTGCCTAAAA ACTTAAACTC ACCAAATGGT
    GTTTTGTTAT CTAGACAGCC GAGTATGAAC CGTGGAGGCT ATATGCCCAC CCCAACAGGG
    GCGAAGGTGG ACTATATTCA GGGGACACCG GTGAGTGTTC ATCTGCAGCC CTCCCTCTCC
    AGACAGAGCA GCTATACCAG TAATGGCACC CTCCCCAGGA CGGGACTAAA GAGGACACCA
    TCCTTAAAAC CTGATGTGCC ACCAAAGCCT TCCTTTGTTC CGCAAACCAC ATCTGTCAGA
    CCACTGAACA AGTACACGTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030105217.1
    CDS8748..11951
    Translation

    Target ORF information:

    RefSeq Version XM_030249357.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249357.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGACA TGGAGGTATC CTCATCTTCT GTTACCACTG TGGCAAGTAG CCCAGAAATT
    ACATCTAAAG TGATTGATAC CTGGAGACCT AAACTGACGA GCTCCCGGAA ATTTGTAGTT
    CAAGATGACC CAAATACTTC TGATTTTACT GATACTATAT CAGGTATCCC AAAGGGTGTA
    CGGTGGGAAG TCCAGTCTGG AGAATCCAAT CAGATGGTCC ACATGAATGT CCTCATCACC
    TGCGTGTTTG CCGCTTTTGT CTTGGGCGCG TTCATCGCAG GAGTGGCCGT GTACTGCTAC
    CGTGACATGT TCGTTCGGAA GAACAGAAAG ATCCATAAAG ACGCAGAATC CGCCCAGTCG
    TGCACAGACT CCAGCGGAAG CTTCGCCAAG CTGAACGGCC TCTTTGACAG CCCCGTCAAG
    GAATACCAGC AGAACATTGA TTCTCCCAAA CTCTACAGCA ACCTGCTGAC CAGTCGGAAG
    GAACTGCCAC CAAACACGGA TACAAAGTCC ATGGCCGTGG ACCACAGAGG CCAGCCTCCC
    GAGCTGGCTG CTCTCCCCAC GCCGGAATCC ACACCTGTCC TCCACCAGAA GACCCTGCAG
    GCCATGAAGA GCCACTCTGA GAAGGCCCAC AGCCACGGTG CTTCAAGGAA AGAACACCCC
    CAGTTTTTTC CTTCTAGTCC TCCACCCCAT TCCCCATTGA GTCACGGGCA TATCCCCAGT
    GCCATCGTTC TTCCAAACGC CACTCACGAC TACAATACAT CCTTCTCCAA CTCGAATGCC
    CACAAAGCCG AAAAGAAGCT TCAGAGCATG GATCACCCTC TTACGAAGTC ATCCAGTAAG
    CGGGAGCACC GGCGGTCTGT GGATTCCAGG AATACTCTCA ATGATCTCCT GAAGCATCTA
    AATGACCCAA ACAGTAACCC CAAAGCCATC CTGGGAGAGA TCCATATGGC TCATCAAACC
    CTCATGCTGG ACCCGGTGGG ACCAATGGCT GAGGTCCCAC CCAAGGTCCC TAACCGGGAG
    GCATCTCTAT ACTCCCCTCC CTCCACACTC CCCAGAAATA GTCCAACCAA GAGAGTAGAT
    GTCCCCACCA CTCCTGGGGT GCCAATGACT TCTCTGGAAA GACAAAGGGG TTATCACAAA
    AATTCCTCCC AGAGGCACTC TATATCTGCC GTGCCTAAAA ACTTAAACTC ACCAAATGGT
    GTTTTGTTAT CTAGACAGCC GAGTATGAAC CGTGGAGGCT ATATGCCCAC CCCAACAGGG
    GCGAAGGTGG ACTATATTCA GGGGACACCG GTGAGTGTTC ATCTGCAGCC CTCCCTCTCC
    AGACAGAGCA GCTATACCAG TAATGGCACC CTCCCCAGGA CGGGACTAAA GAGGACACCA
    TCCTTAAAAC CTGATGTGCC ACCAAAGCCT TCCTTTGTTC CGCAAACCAC ATCTGTCAGA
    CCACTGAACA AGTACACGTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu72912
    Clone ID Related Accession (Same CDS sequence) XM_030249357.1 , XM_017317010.1 , XM_017317010.2
    Accession Version XM_017317010.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3204bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317010.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGACA TGGAGGTATC CTCATCTTCT GTTACCACTG TGGCAAGTAG CCCAGAAATT
    ACATCTAAAG TGATTGATAC CTGGAGACCT AAACTGACGA GCTCCCGGAA ATTTGTAGTT
    CAAGATGACC CAAATACTTC TGATTTTACT GATACTATAT CAGGTATCCC AAAGGGTGTA
    CGGTGGGAAG TCCAGTCTGG AGAATCCAAT CAGATGGTCC ACATGAATGT CCTCATCACC
    TGCGTGTTTG CCGCTTTTGT CTTGGGCGCG TTCATCGCAG GAGTGGCCGT GTACTGCTAC
    CGTGACATGT TCGTTCGGAA GAACAGAAAG ATCCATAAAG ACGCAGAATC CGCCCAGTCG
    TGCACAGACT CCAGCGGAAG CTTCGCCAAG CTGAACGGCC TCTTTGACAG CCCCGTCAAG
    GAATACCAGC AGAACATTGA TTCTCCCAAA CTCTACAGCA ACCTGCTGAC CAGTCGGAAG
    GAACTGCCAC CAAACACGGA TACAAAGTCC ATGGCCGTGG ACCACAGAGG CCAGCCTCCC
    GAGCTGGCTG CTCTCCCCAC GCCGGAATCC ACACCTGTCC TCCACCAGAA GACCCTGCAG
    GCCATGAAGA GCCACTCTGA GAAGGCCCAC AGCCACGGTG CTTCAAGGAA AGAACACCCC
    CAGTTTTTTC CTTCTAGTCC TCCACCCCAT TCCCCATTGA GTCACGGGCA TATCCCCAGT
    GCCATCGTTC TTCCAAACGC CACTCACGAC TACAATACAT CCTTCTCCAA CTCGAATGCC
    CACAAAGCCG AAAAGAAGCT TCAGAGCATG GATCACCCTC TTACGAAGTC ATCCAGTAAG
    CGGGAGCACC GGCGGTCTGT GGATTCCAGG AATACTCTCA ATGATCTCCT GAAGCATCTA
    AATGACCCAA ACAGTAACCC CAAAGCCATC CTGGGAGAGA TCCATATGGC TCATCAAACC
    CTCATGCTGG ACCCGGTGGG ACCAATGGCT GAGGTCCCAC CCAAGGTCCC TAACCGGGAG
    GCATCTCTAT ACTCCCCTCC CTCCACACTC CCCAGAAATA GTCCAACCAA GAGAGTAGAT
    GTCCCCACCA CTCCTGGGGT GCCAATGACT TCTCTGGAAA GACAAAGGGG TTATCACAAA
    AATTCCTCCC AGAGGCACTC TATATCTGCC GTGCCTAAAA ACTTAAACTC ACCAAATGGT
    GTTTTGTTAT CTAGACAGCC GAGTATGAAC CGTGGAGGCT ATATGCCCAC CCCAACAGGG
    GCGAAGGTGG ACTATATTCA GGGGACACCG GTGAGTGTTC ATCTGCAGCC CTCCCTCTCC
    AGACAGAGCA GCTATACCAG TAATGGCACC CTCCCCAGGA CGGGACTAAA GAGGACACCA
    TCCTTAAAAC CTGATGTGCC ACCAAAGCCT TCCTTTGTTC CGCAAACCAC ATCTGTCAGA
    CCACTGAACA AGTACACGTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172499.1
    CDS830..4033
    Translation

    Target ORF information:

    RefSeq Version XM_017317010.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317010.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGACA TGGAGGTATC CTCATCTTCT GTTACCACTG TGGCAAGTAG CCCAGAAATT
    ACATCTAAAG TGATTGATAC CTGGAGACCT AAACTGACGA GCTCCCGGAA ATTTGTAGTT
    CAAGATGACC CAAATACTTC TGATTTTACT GATACTATAT CAGGTATCCC AAAGGGTGTA
    CGGTGGGAAG TCCAGTCTGG AGAATCCAAT CAGATGGTCC ACATGAATGT CCTCATCACC
    TGCGTGTTTG CCGCTTTTGT CTTGGGCGCG TTCATCGCAG GAGTGGCCGT GTACTGCTAC
    CGTGACATGT TCGTTCGGAA GAACAGAAAG ATCCATAAAG ACGCAGAATC CGCCCAGTCG
    TGCACAGACT CCAGCGGAAG CTTCGCCAAG CTGAACGGCC TCTTTGACAG CCCCGTCAAG
    GAATACCAGC AGAACATTGA TTCTCCCAAA CTCTACAGCA ACCTGCTGAC CAGTCGGAAG
    GAACTGCCAC CAAACACGGA TACAAAGTCC ATGGCCGTGG ACCACAGAGG CCAGCCTCCC
    GAGCTGGCTG CTCTCCCCAC GCCGGAATCC ACACCTGTCC TCCACCAGAA GACCCTGCAG
    GCCATGAAGA GCCACTCTGA GAAGGCCCAC AGCCACGGTG CTTCAAGGAA AGAACACCCC
    CAGTTTTTTC CTTCTAGTCC TCCACCCCAT TCCCCATTGA GTCACGGGCA TATCCCCAGT
    GCCATCGTTC TTCCAAACGC CACTCACGAC TACAATACAT CCTTCTCCAA CTCGAATGCC
    CACAAAGCCG AAAAGAAGCT TCAGAGCATG GATCACCCTC TTACGAAGTC ATCCAGTAAG
    CGGGAGCACC GGCGGTCTGT GGATTCCAGG AATACTCTCA ATGATCTCCT GAAGCATCTA
    AATGACCCAA ACAGTAACCC CAAAGCCATC CTGGGAGAGA TCCATATGGC TCATCAAACC
    CTCATGCTGG ACCCGGTGGG ACCAATGGCT GAGGTCCCAC CCAAGGTCCC TAACCGGGAG
    GCATCTCTAT ACTCCCCTCC CTCCACACTC CCCAGAAATA GTCCAACCAA GAGAGTAGAT
    GTCCCCACCA CTCCTGGGGT GCCAATGACT TCTCTGGAAA GACAAAGGGG TTATCACAAA
    AATTCCTCCC AGAGGCACTC TATATCTGCC GTGCCTAAAA ACTTAAACTC ACCAAATGGT
    GTTTTGTTAT CTAGACAGCC GAGTATGAAC CGTGGAGGCT ATATGCCCAC CCCAACAGGG
    GCGAAGGTGG ACTATATTCA GGGGACACCG GTGAGTGTTC ATCTGCAGCC CTCCCTCTCC
    AGACAGAGCA GCTATACCAG TAATGGCACC CTCCCCAGGA CGGGACTAAA GAGGACACCA
    TCCTTAAAAC CTGATGTGCC ACCAAAGCCT TCCTTTGTTC CGCAAACCAC ATCTGTCAGA
    CCACTGAACA AGTACACGTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu72913
    Clone ID Related Accession (Same CDS sequence) XM_017317021.2 , XM_030249368.1 , XM_017317021.1
    Accession Version XM_017317021.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3054bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317021.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGGTGTA CGGTGGGAAG TCCAGTCTGG AGAATCCAAT
    CAGATGGTCC ACATGAATGT CCTCATCACC TGCGTGTTTG CCGCTTTTGT CTTGGGCGCG
    TTCATCGCAG GAGTGGCCGT GTACTGCTAC CGTGACATGT TCGTTCGGAA GAACAGAAAG
    ATCCATAAAG ACGCAGAATC CGCCCAGTCG TGCACAGACT CCAGCGGAAG CTTCGCCAAG
    CTGAACGGCC TCTTTGACAG CCCCGTCAAG GAATACCAGC AGAACATTGA TTCTCCCAAA
    CTCTACAGCA ACCTGCTGAC CAGTCGGAAG GAACTGCCAC CAAACACGGA TACAAAGTCC
    ATGGCCGTGG ACCACAGAGG CCAGCCTCCC GAGCTGGCTG CTCTCCCCAC GCCGGAATCC
    ACACCTGTCC TCCACCAGAA GACCCTGCAG GCCATGAAGA GCCACTCTGA GAAGGCCCAC
    AGCCACGGTG CTTCAAGGAA AGAACACCCC CAGTTTTTTC CTTCTAGTCC TCCACCCCAT
    TCCCCATTGA GTCACGGGCA TATCCCCAGT GCCATCGTTC TTCCAAACGC CACTCACGAC
    TACAATACAT CCTTCTCCAA CTCGAATGCC CACAAAGCCG AAAAGAAGCT TCAGAGCATG
    GATCACCCTC TTACGAAGTC ATCCAGTAAG CGGGAGCACC GGCGGTCTGT GGATTCCAGG
    AATACTCTCA ATGATCTCCT GAAGCATCTA AATGACCCAA ACAGTAACCC CAAAGCCATC
    CTGGGAGAGA TCCATATGGC TCATCAAACC CTCATGCTGG ACCCGGTGGG ACCAATGGCT
    GAGGTCCCAC CCAAGGTCCC TAACCGGGAG GCATCTCTAT ACTCCCCTCC CTCCACACTC
    CCCAGAAATA GTCCAACCAA GAGAGTAGAT GTCCCCACCA CTCCTGGGGT GCCAATGACT
    TCTCTGGAAA GACAAAGGGG TTATCACAAA AATTCCTCCC AGAGGCACTC TATATCTGCC
    GTGCCTAAAA ACTTAAACTC ACCAAATGGT GTTTTGTTAT CTAGACAGCC GAGTATGAAC
    CGTGGAGGCT ATATGCCCAC CCCAACAGGG GCGAAGGTGG ACTATATTCA GGGGACACCG
    GTGAGTGTTC ATCTGCAGCC CTCCCTCTCC AGACAGAGCA GCTATACCAG TAATGGCACC
    CTCCCCAGGA CGGGACTAAA GAGGACACCA TCCTTAAAAC CTGATGTGCC ACCAAAGCCT
    TCCTTTGTTC CGCAAACCAC ATCTGTCAGA CCACTGAACA AGTACACGTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172510.1
    CDS830..3883
    Translation

    Target ORF information:

    RefSeq Version XM_017317021.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317021.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGGTGTA CGGTGGGAAG TCCAGTCTGG AGAATCCAAT
    CAGATGGTCC ACATGAATGT CCTCATCACC TGCGTGTTTG CCGCTTTTGT CTTGGGCGCG
    TTCATCGCAG GAGTGGCCGT GTACTGCTAC CGTGACATGT TCGTTCGGAA GAACAGAAAG
    ATCCATAAAG ACGCAGAATC CGCCCAGTCG TGCACAGACT CCAGCGGAAG CTTCGCCAAG
    CTGAACGGCC TCTTTGACAG CCCCGTCAAG GAATACCAGC AGAACATTGA TTCTCCCAAA
    CTCTACAGCA ACCTGCTGAC CAGTCGGAAG GAACTGCCAC CAAACACGGA TACAAAGTCC
    ATGGCCGTGG ACCACAGAGG CCAGCCTCCC GAGCTGGCTG CTCTCCCCAC GCCGGAATCC
    ACACCTGTCC TCCACCAGAA GACCCTGCAG GCCATGAAGA GCCACTCTGA GAAGGCCCAC
    AGCCACGGTG CTTCAAGGAA AGAACACCCC CAGTTTTTTC CTTCTAGTCC TCCACCCCAT
    TCCCCATTGA GTCACGGGCA TATCCCCAGT GCCATCGTTC TTCCAAACGC CACTCACGAC
    TACAATACAT CCTTCTCCAA CTCGAATGCC CACAAAGCCG AAAAGAAGCT TCAGAGCATG
    GATCACCCTC TTACGAAGTC ATCCAGTAAG CGGGAGCACC GGCGGTCTGT GGATTCCAGG
    AATACTCTCA ATGATCTCCT GAAGCATCTA AATGACCCAA ACAGTAACCC CAAAGCCATC
    CTGGGAGAGA TCCATATGGC TCATCAAACC CTCATGCTGG ACCCGGTGGG ACCAATGGCT
    GAGGTCCCAC CCAAGGTCCC TAACCGGGAG GCATCTCTAT ACTCCCCTCC CTCCACACTC
    CCCAGAAATA GTCCAACCAA GAGAGTAGAT GTCCCCACCA CTCCTGGGGT GCCAATGACT
    TCTCTGGAAA GACAAAGGGG TTATCACAAA AATTCCTCCC AGAGGCACTC TATATCTGCC
    GTGCCTAAAA ACTTAAACTC ACCAAATGGT GTTTTGTTAT CTAGACAGCC GAGTATGAAC
    CGTGGAGGCT ATATGCCCAC CCCAACAGGG GCGAAGGTGG ACTATATTCA GGGGACACCG
    GTGAGTGTTC ATCTGCAGCC CTCCCTCTCC AGACAGAGCA GCTATACCAG TAATGGCACC
    CTCCCCAGGA CGGGACTAAA GAGGACACCA TCCTTAAAAC CTGATGTGCC ACCAAAGCCT
    TCCTTTGTTC CGCAAACCAC ATCTGTCAGA CCACTGAACA AGTACACGTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu72913
    Clone ID Related Accession (Same CDS sequence) XM_017317021.2 , XM_030249368.1 , XM_017317021.1
    Accession Version XM_030249368.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3054bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGGTGTA CGGTGGGAAG TCCAGTCTGG AGAATCCAAT
    CAGATGGTCC ACATGAATGT CCTCATCACC TGCGTGTTTG CCGCTTTTGT CTTGGGCGCG
    TTCATCGCAG GAGTGGCCGT GTACTGCTAC CGTGACATGT TCGTTCGGAA GAACAGAAAG
    ATCCATAAAG ACGCAGAATC CGCCCAGTCG TGCACAGACT CCAGCGGAAG CTTCGCCAAG
    CTGAACGGCC TCTTTGACAG CCCCGTCAAG GAATACCAGC AGAACATTGA TTCTCCCAAA
    CTCTACAGCA ACCTGCTGAC CAGTCGGAAG GAACTGCCAC CAAACACGGA TACAAAGTCC
    ATGGCCGTGG ACCACAGAGG CCAGCCTCCC GAGCTGGCTG CTCTCCCCAC GCCGGAATCC
    ACACCTGTCC TCCACCAGAA GACCCTGCAG GCCATGAAGA GCCACTCTGA GAAGGCCCAC
    AGCCACGGTG CTTCAAGGAA AGAACACCCC CAGTTTTTTC CTTCTAGTCC TCCACCCCAT
    TCCCCATTGA GTCACGGGCA TATCCCCAGT GCCATCGTTC TTCCAAACGC CACTCACGAC
    TACAATACAT CCTTCTCCAA CTCGAATGCC CACAAAGCCG AAAAGAAGCT TCAGAGCATG
    GATCACCCTC TTACGAAGTC ATCCAGTAAG CGGGAGCACC GGCGGTCTGT GGATTCCAGG
    AATACTCTCA ATGATCTCCT GAAGCATCTA AATGACCCAA ACAGTAACCC CAAAGCCATC
    CTGGGAGAGA TCCATATGGC TCATCAAACC CTCATGCTGG ACCCGGTGGG ACCAATGGCT
    GAGGTCCCAC CCAAGGTCCC TAACCGGGAG GCATCTCTAT ACTCCCCTCC CTCCACACTC
    CCCAGAAATA GTCCAACCAA GAGAGTAGAT GTCCCCACCA CTCCTGGGGT GCCAATGACT
    TCTCTGGAAA GACAAAGGGG TTATCACAAA AATTCCTCCC AGAGGCACTC TATATCTGCC
    GTGCCTAAAA ACTTAAACTC ACCAAATGGT GTTTTGTTAT CTAGACAGCC GAGTATGAAC
    CGTGGAGGCT ATATGCCCAC CCCAACAGGG GCGAAGGTGG ACTATATTCA GGGGACACCG
    GTGAGTGTTC ATCTGCAGCC CTCCCTCTCC AGACAGAGCA GCTATACCAG TAATGGCACC
    CTCCCCAGGA CGGGACTAAA GAGGACACCA TCCTTAAAAC CTGATGTGCC ACCAAAGCCT
    TCCTTTGTTC CGCAAACCAC ATCTGTCAGA CCACTGAACA AGTACACGTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030105228.1
    CDS8748..11801
    Translation

    Target ORF information:

    RefSeq Version XM_030249368.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249368.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGGTGTA CGGTGGGAAG TCCAGTCTGG AGAATCCAAT
    CAGATGGTCC ACATGAATGT CCTCATCACC TGCGTGTTTG CCGCTTTTGT CTTGGGCGCG
    TTCATCGCAG GAGTGGCCGT GTACTGCTAC CGTGACATGT TCGTTCGGAA GAACAGAAAG
    ATCCATAAAG ACGCAGAATC CGCCCAGTCG TGCACAGACT CCAGCGGAAG CTTCGCCAAG
    CTGAACGGCC TCTTTGACAG CCCCGTCAAG GAATACCAGC AGAACATTGA TTCTCCCAAA
    CTCTACAGCA ACCTGCTGAC CAGTCGGAAG GAACTGCCAC CAAACACGGA TACAAAGTCC
    ATGGCCGTGG ACCACAGAGG CCAGCCTCCC GAGCTGGCTG CTCTCCCCAC GCCGGAATCC
    ACACCTGTCC TCCACCAGAA GACCCTGCAG GCCATGAAGA GCCACTCTGA GAAGGCCCAC
    AGCCACGGTG CTTCAAGGAA AGAACACCCC CAGTTTTTTC CTTCTAGTCC TCCACCCCAT
    TCCCCATTGA GTCACGGGCA TATCCCCAGT GCCATCGTTC TTCCAAACGC CACTCACGAC
    TACAATACAT CCTTCTCCAA CTCGAATGCC CACAAAGCCG AAAAGAAGCT TCAGAGCATG
    GATCACCCTC TTACGAAGTC ATCCAGTAAG CGGGAGCACC GGCGGTCTGT GGATTCCAGG
    AATACTCTCA ATGATCTCCT GAAGCATCTA AATGACCCAA ACAGTAACCC CAAAGCCATC
    CTGGGAGAGA TCCATATGGC TCATCAAACC CTCATGCTGG ACCCGGTGGG ACCAATGGCT
    GAGGTCCCAC CCAAGGTCCC TAACCGGGAG GCATCTCTAT ACTCCCCTCC CTCCACACTC
    CCCAGAAATA GTCCAACCAA GAGAGTAGAT GTCCCCACCA CTCCTGGGGT GCCAATGACT
    TCTCTGGAAA GACAAAGGGG TTATCACAAA AATTCCTCCC AGAGGCACTC TATATCTGCC
    GTGCCTAAAA ACTTAAACTC ACCAAATGGT GTTTTGTTAT CTAGACAGCC GAGTATGAAC
    CGTGGAGGCT ATATGCCCAC CCCAACAGGG GCGAAGGTGG ACTATATTCA GGGGACACCG
    GTGAGTGTTC ATCTGCAGCC CTCCCTCTCC AGACAGAGCA GCTATACCAG TAATGGCACC
    CTCCCCAGGA CGGGACTAAA GAGGACACCA TCCTTAAAAC CTGATGTGCC ACCAAAGCCT
    TCCTTTGTTC CGCAAACCAC ATCTGTCAGA CCACTGAACA AGTACACGTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu80174
    Clone ID Related Accession (Same CDS sequence) XM_017317014.1 , XM_017317014.2 , XM_030249365.1 , XM_030249366.1
    Accession Version XM_017317014.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3153bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT
    GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA
    TCAGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC
    CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG
    TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC
    GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC
    CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC
    AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC
    CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG
    ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA
    GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT
    ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC
    TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA
    TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG
    AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT
    CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT
    AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG
    AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT
    TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA
    CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC
    CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC
    TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG
    AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA
    TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172503.1
    CDS832..3984
    Translation

    Target ORF information:

    RefSeq Version XM_017317014.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317014.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT
    GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA
    TCAGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC
    CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG
    TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC
    GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC
    CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC
    AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC
    CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG
    ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA
    GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT
    ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC
    TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA
    TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG
    AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT
    CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT
    AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG
    AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT
    TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA
    CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC
    CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC
    TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG
    AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA
    TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu80174
    Clone ID Related Accession (Same CDS sequence) XM_017317014.1 , XM_017317014.2 , XM_030249365.1 , XM_030249366.1
    Accession Version XM_017317014.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3153bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317014.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT
    GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA
    TCAGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC
    CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG
    TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC
    GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC
    CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC
    AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC
    CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG
    ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA
    GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT
    ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC
    TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA
    TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG
    AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT
    CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT
    AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG
    AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT
    TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA
    CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC
    CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC
    TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG
    AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA
    TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172503.1
    CDS830..3982
    Translation

    Target ORF information:

    RefSeq Version XM_017317014.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317014.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT
    GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA
    TCAGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC
    CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG
    TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC
    GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC
    CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC
    AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC
    CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG
    ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA
    GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT
    ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC
    TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA
    TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG
    AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT
    CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT
    AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG
    AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT
    TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA
    CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC
    CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC
    TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG
    AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA
    TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu80174
    Clone ID Related Accession (Same CDS sequence) XM_017317014.1 , XM_017317014.2 , XM_030249365.1 , XM_030249366.1
    Accession Version XM_030249365.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3153bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT
    GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA
    TCAGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC
    CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG
    TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC
    GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC
    CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC
    AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC
    CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG
    ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA
    GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT
    ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC
    TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA
    TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG
    AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT
    CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT
    AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG
    AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT
    TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA
    CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC
    CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC
    TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG
    AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA
    TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030105225.1
    CDS605..3757
    Translation

    Target ORF information:

    RefSeq Version XM_030249365.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249365.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT
    GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA
    TCAGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC
    CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG
    TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC
    GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC
    CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC
    AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC
    CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG
    ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA
    GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT
    ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC
    TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA
    TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG
    AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT
    CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT
    AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG
    AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT
    TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA
    CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC
    CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC
    TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG
    AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA
    TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu80174
    Clone ID Related Accession (Same CDS sequence) XM_017317014.1 , XM_017317014.2 , XM_030249365.1 , XM_030249366.1
    Accession Version XM_030249366.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3153bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT
    GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA
    TCAGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC
    CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG
    TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC
    GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC
    CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC
    AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC
    CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG
    ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA
    GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT
    ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC
    TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA
    TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG
    AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT
    CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT
    AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG
    AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT
    TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA
    CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC
    CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC
    TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG
    AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA
    TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030105226.1
    CDS8748..11900
    Translation

    Target ORF information:

    RefSeq Version XM_030249366.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249366.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT
    GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA
    TCAGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC
    CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG
    TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC
    GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC
    CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC
    AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC
    CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG
    ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA
    GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT
    ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC
    TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA
    TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG
    AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT
    CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT
    AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG
    AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT
    TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA
    CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC
    CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC
    TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG
    AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA
    TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu87775
    Clone ID Related Accession (Same CDS sequence) XM_030249358.1
    Accession Version XM_030249358.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3192bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGACA TGGAGGTATC CTCATCTTCT GTTACCACTG TGGCAAGTAG CCCAGAAATT
    ACATCTAAAG TGATTGATAC CTGGAGACCT AAACTGACGA GCTCCCGGAA ATTTGTAGTT
    CAAGATGACC CAAATACTTC TGATTTTACT GATACTATAT CAGGTGTACG GTGGGAAGTC
    CAGTCTGGAG AATCCAATCA GATGGTCCAC ATGAATGTCC TCATCACCTG CGTGTTTGCC
    GCTTTTGTCT TGGGCGCGTT CATCGCAGGA GTGGCCGTGT ACTGCTACCG TGACATGTTC
    GTTCGGAAGA ACAGAAAGAT CCATAAAGAC GCAGAATCCG CCCAGTCGTG CACAGACTCC
    AGCGGAAGCT TCGCCAAGCT GAACGGCCTC TTTGACAGCC CCGTCAAGGA ATACCAGCAG
    AACATTGATT CTCCCAAACT CTACAGCAAC CTGCTGACCA GTCGGAAGGA ACTGCCACCA
    AACACGGATA CAAAGTCCAT GGCCGTGGAC CACAGAGGCC AGCCTCCCGA GCTGGCTGCT
    CTCCCCACGC CGGAATCCAC ACCTGTCCTC CACCAGAAGA CCCTGCAGGC CATGAAGAGC
    CACTCTGAGA AGGCCCACAG CCACGGTGCT TCAAGGAAAG AACACCCCCA GTTTTTTCCT
    TCTAGTCCTC CACCCCATTC CCCATTGAGT CACGGGCATA TCCCCAGTGC CATCGTTCTT
    CCAAACGCCA CTCACGACTA CAATACATCC TTCTCCAACT CGAATGCCCA CAAAGCCGAA
    AAGAAGCTTC AGAGCATGGA TCACCCTCTT ACGAAGTCAT CCAGTAAGCG GGAGCACCGG
    CGGTCTGTGG ATTCCAGGAA TACTCTCAAT GATCTCCTGA AGCATCTAAA TGACCCAAAC
    AGTAACCCCA AAGCCATCCT GGGAGAGATC CATATGGCTC ATCAAACCCT CATGCTGGAC
    CCGGTGGGAC CAATGGCTGA GGTCCCACCC AAGGTCCCTA ACCGGGAGGC ATCTCTATAC
    TCCCCTCCCT CCACACTCCC CAGAAATAGT CCAACCAAGA GAGTAGATGT CCCCACCACT
    CCTGGGGTGC CAATGACTTC TCTGGAAAGA CAAAGGGGTT ATCACAAAAA TTCCTCCCAG
    AGGCACTCTA TATCTGCCGT GCCTAAAAAC TTAAACTCAC CAAATGGTGT TTTGTTATCT
    AGACAGCCGA GTATGAACCG TGGAGGCTAT ATGCCCACCC CAACAGGGGC GAAGGTGGAC
    TATATTCAGG GGACACCGGT GAGTGTTCAT CTGCAGCCCT CCCTCTCCAG ACAGAGCAGC
    TATACCAGTA ATGGCACCCT CCCCAGGACG GGACTAAAGA GGACACCATC CTTAAAACCT
    GATGTGCCAC CAAAGCCTTC CTTTGTTCCG CAAACCACAT CTGTCAGACC ACTGAACAAG
    TACACGTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030105218.1
    CDS830..4021
    Translation

    Target ORF information:

    RefSeq Version XM_030249358.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249358.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGACA TGGAGGTATC CTCATCTTCT GTTACCACTG TGGCAAGTAG CCCAGAAATT
    ACATCTAAAG TGATTGATAC CTGGAGACCT AAACTGACGA GCTCCCGGAA ATTTGTAGTT
    CAAGATGACC CAAATACTTC TGATTTTACT GATACTATAT CAGGTGTACG GTGGGAAGTC
    CAGTCTGGAG AATCCAATCA GATGGTCCAC ATGAATGTCC TCATCACCTG CGTGTTTGCC
    GCTTTTGTCT TGGGCGCGTT CATCGCAGGA GTGGCCGTGT ACTGCTACCG TGACATGTTC
    GTTCGGAAGA ACAGAAAGAT CCATAAAGAC GCAGAATCCG CCCAGTCGTG CACAGACTCC
    AGCGGAAGCT TCGCCAAGCT GAACGGCCTC TTTGACAGCC CCGTCAAGGA ATACCAGCAG
    AACATTGATT CTCCCAAACT CTACAGCAAC CTGCTGACCA GTCGGAAGGA ACTGCCACCA
    AACACGGATA CAAAGTCCAT GGCCGTGGAC CACAGAGGCC AGCCTCCCGA GCTGGCTGCT
    CTCCCCACGC CGGAATCCAC ACCTGTCCTC CACCAGAAGA CCCTGCAGGC CATGAAGAGC
    CACTCTGAGA AGGCCCACAG CCACGGTGCT TCAAGGAAAG AACACCCCCA GTTTTTTCCT
    TCTAGTCCTC CACCCCATTC CCCATTGAGT CACGGGCATA TCCCCAGTGC CATCGTTCTT
    CCAAACGCCA CTCACGACTA CAATACATCC TTCTCCAACT CGAATGCCCA CAAAGCCGAA
    AAGAAGCTTC AGAGCATGGA TCACCCTCTT ACGAAGTCAT CCAGTAAGCG GGAGCACCGG
    CGGTCTGTGG ATTCCAGGAA TACTCTCAAT GATCTCCTGA AGCATCTAAA TGACCCAAAC
    AGTAACCCCA AAGCCATCCT GGGAGAGATC CATATGGCTC ATCAAACCCT CATGCTGGAC
    CCGGTGGGAC CAATGGCTGA GGTCCCACCC AAGGTCCCTA ACCGGGAGGC ATCTCTATAC
    TCCCCTCCCT CCACACTCCC CAGAAATAGT CCAACCAAGA GAGTAGATGT CCCCACCACT
    CCTGGGGTGC CAATGACTTC TCTGGAAAGA CAAAGGGGTT ATCACAAAAA TTCCTCCCAG
    AGGCACTCTA TATCTGCCGT GCCTAAAAAC TTAAACTCAC CAAATGGTGT TTTGTTATCT
    AGACAGCCGA GTATGAACCG TGGAGGCTAT ATGCCCACCC CAACAGGGGC GAAGGTGGAC
    TATATTCAGG GGACACCGGT GAGTGTTCAT CTGCAGCCCT CCCTCTCCAG ACAGAGCAGC
    TATACCAGTA ATGGCACCCT CCCCAGGACG GGACTAAAGA GGACACCATC CTTAAAACCT
    GATGTGCCAC CAAAGCCTTC CTTTGTTCCG CAAACCACAT CTGTCAGACC ACTGAACAAG
    TACACGTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20434
    Clone ID Related Accession (Same CDS sequence) XM_017317019.2 , XM_017317019.1 , NM_199239.3
    Accession Version NM_199239.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3093bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-10
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform Sema6D-5 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC060680.1, AB091534.1 and AL935323.6. On Mar 21, 2014 this sequence version replaced NM_199239.2. Transcript Variant: This variant (5) contains an additional in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (Sema6D-5) that is longer than isoform Sema6D-1. The 5' UTR is incomplete because the evidence for this variant is for the CDS only, and multiple possible 5' UTR exon combinations exist. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB091534.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC
    CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG
    TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC
    GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC
    CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC
    AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC
    CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG
    ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA
    GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT
    ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC
    TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA
    TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG
    AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT
    CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT
    AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG
    AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT
    TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA
    CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC
    CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC
    TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG
    AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA
    TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_954709.1
    CDS55..3147
    Translation

    Target ORF information:

    RefSeq Version NM_199239.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_199239.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC
    CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG
    TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC
    GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC
    CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC
    AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC
    CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG
    ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA
    GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT
    ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC
    TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA
    TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG
    AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT
    CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT
    AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG
    AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT
    TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA
    CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC
    CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC
    TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG
    AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA
    TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20449
    Clone ID Related Accession (Same CDS sequence) XM_030249369.1 , XM_030249371.1 , NM_172537.4
    Accession Version NM_172537.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-10
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform Sema6D-1 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB091532.1, BC060680.1 and AL935323.6. On Mar 21, 2014 this sequence version replaced NM_172537.3. Transcript Variant: This variant (1) encodes isoform Sema6D-1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB091532.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGGTG TACGGTGGGA AGTCCAGTCT GGAGAATCCA ATCAGATGGT CCACATGAAT
    GTCCTCATCA CCTGCGTGTT TGCCGCTTTT GTCTTGGGCG CGTTCATCGC AGGAGTGGCC
    GTGTACTGCT ACCGTGACAT GTTCGTTCGG AAGAACAGAA AGATCCATAA AGACGCAGAA
    TCCGCCCAGT CGTGCACAGA CTCCAGCGGA AGCTTCGCCA AGCTGAACGG CCTCTTTGAC
    AGCCCCGTCA AGGAATACCA GCAGAACATT GATTCTCCCA AACTCTACAG CAACCTGCTG
    ACCAGTCGGA AGGAACTGCC ACCAAACACG GATACAAAGT CCATGGCCGT GGACCACAGA
    GGCCAGCCTC CCGAGCTGGC TGCTCTCCCC ACGCCGGAAT CCACACCTGT CCTCCACCAG
    AAGACCCTGC AGGCCATGAA GAGCCACTCT GAGAAGGCCC ACAGCCACGG TGCTTCAAGG
    AAAGAACACC CCCAGTTTTT TCCTTCTAGT CCTCCACCCC ATTCCCCATT GAGTCACGGG
    CATATCCCCA GTGCCATCGT TCTTCCAAAC GCCACTCACG ACTACAATAC ATCCTTCTCC
    AACTCGAATG CCCACAAAGC CGAAAAGAAG CTTCAGAGCA TGGATCACCC TCTTACGAAG
    TCATCCAGTA AGCGGGAGCA CCGGCGGTCT GTGGATTCCA GGAATACTCT CAATGATCTC
    CTGAAGCATC TAAATGACCC AAACAGTAAC CCCAAAGCCA TCCTGGGAGA GATCCATATG
    GCTCATCAAA CCCTCATGCT GGACCCGGTG GGACCAATGG CTGAGGTCCC ACCCAAGGTC
    CCTAACCGGG AGGCATCTCT ATACTCCCCT CCCTCCACAC TCCCCAGAAA TAGTCCAACC
    AAGAGAGTAG ATGTCCCCAC CACTCCTGGG GTGCCAATGA CTTCTCTGGA AAGACAAAGG
    GGTTATCACA AAAATTCCTC CCAGAGGCAC TCTATATCTG CCGTGCCTAA AAACTTAAAC
    TCACCAAATG GTGTTTTGTT ATCTAGACAG CCGAGTATGA ACCGTGGAGG CTATATGCCC
    ACCCCAACAG GGGCGAAGGT GGACTATATT CAGGGGACAC CGGTGAGTGT TCATCTGCAG
    CCCTCCCTCT CCAGACAGAG CAGCTATACC AGTAATGGCA CCCTCCCCAG GACGGGACTA
    AAGAGGACAC CATCCTTAAA ACCTGATGTG CCACCAAAGC CTTCCTTTGT TCCGCAAACC
    ACATCTGTCA GACCACTGAA CAAGTACACG TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_766125.2
    CDS820..3855
    Translation

    Target ORF information:

    RefSeq Version NM_172537.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_172537.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGGTG TACGGTGGGA AGTCCAGTCT GGAGAATCCA ATCAGATGGT CCACATGAAT
    GTCCTCATCA CCTGCGTGTT TGCCGCTTTT GTCTTGGGCG CGTTCATCGC AGGAGTGGCC
    GTGTACTGCT ACCGTGACAT GTTCGTTCGG AAGAACAGAA AGATCCATAA AGACGCAGAA
    TCCGCCCAGT CGTGCACAGA CTCCAGCGGA AGCTTCGCCA AGCTGAACGG CCTCTTTGAC
    AGCCCCGTCA AGGAATACCA GCAGAACATT GATTCTCCCA AACTCTACAG CAACCTGCTG
    ACCAGTCGGA AGGAACTGCC ACCAAACACG GATACAAAGT CCATGGCCGT GGACCACAGA
    GGCCAGCCTC CCGAGCTGGC TGCTCTCCCC ACGCCGGAAT CCACACCTGT CCTCCACCAG
    AAGACCCTGC AGGCCATGAA GAGCCACTCT GAGAAGGCCC ACAGCCACGG TGCTTCAAGG
    AAAGAACACC CCCAGTTTTT TCCTTCTAGT CCTCCACCCC ATTCCCCATT GAGTCACGGG
    CATATCCCCA GTGCCATCGT TCTTCCAAAC GCCACTCACG ACTACAATAC ATCCTTCTCC
    AACTCGAATG CCCACAAAGC CGAAAAGAAG CTTCAGAGCA TGGATCACCC TCTTACGAAG
    TCATCCAGTA AGCGGGAGCA CCGGCGGTCT GTGGATTCCA GGAATACTCT CAATGATCTC
    CTGAAGCATC TAAATGACCC AAACAGTAAC CCCAAAGCCA TCCTGGGAGA GATCCATATG
    GCTCATCAAA CCCTCATGCT GGACCCGGTG GGACCAATGG CTGAGGTCCC ACCCAAGGTC
    CCTAACCGGG AGGCATCTCT ATACTCCCCT CCCTCCACAC TCCCCAGAAA TAGTCCAACC
    AAGAGAGTAG ATGTCCCCAC CACTCCTGGG GTGCCAATGA CTTCTCTGGA AAGACAAAGG
    GGTTATCACA AAAATTCCTC CCAGAGGCAC TCTATATCTG CCGTGCCTAA AAACTTAAAC
    TCACCAAATG GTGTTTTGTT ATCTAGACAG CCGAGTATGA ACCGTGGAGG CTATATGCCC
    ACCCCAACAG GGGCGAAGGT GGACTATATT CAGGGGACAC CGGTGAGTGT TCATCTGCAG
    CCCTCCCTCT CCAGACAGAG CAGCTATACC AGTAATGGCA CCCTCCCCAG GACGGGACTA
    AAGAGGACAC CATCCTTAAA ACCTGATGTG CCACCAAAGC CTTCCTTTGT TCCGCAAACC
    ACATCTGTCA GACCACTGAA CAAGTACACG TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20490
    Clone ID Related Accession (Same CDS sequence) XM_030249343.1 , XM_030249345.1 , XM_017317001.1 , XM_017317001.2 , NM_199241.3
    Accession Version NM_199241.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-10
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform Sema6D-4 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC060680.1, AB091536.1 and AL935323.6. On Mar 21, 2014 this sequence version replaced NM_199241.2. Transcript Variant: This variant (4) uses an alternate in-frame splice site and contains two additional in-frame exons in the central coding region, compared to variant 1. The encoded isoform (Sema6D-4) is longer than isoform Sema6D-1. The 5' UTR is incomplete because the evidence for this variant is for the CDS only, and multiple possible 5' UTR exon combinations exist. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB091536.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG
    GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC
    TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA
    GGTATCCCAA AGGGTGTACG GTGGGAAGTC CAGTCTGGAG AATCCAATCA GATGGTCCAC
    ATGAATGTCC TCATCACCTG CGTGTTTGCC GCTTTTGTCT TGGGCGCGTT CATCGCAGGA
    GTGGCCGTGT ACTGCTACCG TGACATGTTC GTTCGGAAGA ACAGAAAGAT CCATAAAGAC
    GCAGAATCCG CCCAGTCGTG CACAGACTCC AGCGGAAGCT TCGCCAAGCT GAACGGCCTC
    TTTGACAGCC CCGTCAAGGA ATACCAGCAG AACATTGATT CTCCCAAACT CTACAGCAAC
    CTGCTGACCA GTCGGAAGGA ACTGCCACCA AACACGGATA CAAAGTCCAT GGCCGTGGAC
    CACAGAGGCC AGCCTCCCGA GCTGGCTGCT CTCCCCACGC CGGAATCCAC ACCTGTCCTC
    CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCTGAGA AGGCCCACAG CCACGGTGCT
    TCAAGGAAAG AACACCCCCA GTTTTTTCCT TCTAGTCCTC CACCCCATTC CCCATTGAGT
    CACGGGCATA TCCCCAGTGC CATCGTTCTT CCAAACGCCA CTCACGACTA CAATACATCC
    TTCTCCAACT CGAATGCCCA CAAAGCCGAA AAGAAGCTTC AGAGCATGGA TCACCCTCTT
    ACGAAGTCAT CCAGTAAGCG GGAGCACCGG CGGTCTGTGG ATTCCAGGAA TACTCTCAAT
    GATCTCCTGA AGCATCTAAA TGACCCAAAC AGTAACCCCA AAGCCATCCT GGGAGAGATC
    CATATGGCTC ATCAAACCCT CATGCTGGAC CCGGTGGGAC CAATGGCTGA GGTCCCACCC
    AAGGTCCCTA ACCGGGAGGC ATCTCTATAC TCCCCTCCCT CCACACTCCC CAGAAATAGT
    CCAACCAAGA GAGTAGATGT CCCCACCACT CCTGGGGTGC CAATGACTTC TCTGGAAAGA
    CAAAGGGGTT ATCACAAAAA TTCCTCCCAG AGGCACTCTA TATCTGCCGT GCCTAAAAAC
    TTAAACTCAC CAAATGGTGT TTTGTTATCT AGACAGCCGA GTATGAACCG TGGAGGCTAT
    ATGCCCACCC CAACAGGGGC GAAGGTGGAC TATATTCAGG GGACACCGGT GAGTGTTCAT
    CTGCAGCCCT CCCTCTCCAG ACAGAGCAGC TATACCAGTA ATGGCACCCT CCCCAGGACG
    GGACTAAAGA GGACACCATC CTTAAAACCT GATGTGCCAC CAAAGCCTTC CTTTGTTCCG
    CAAACCACAT CTGTCAGACC ACTGAACAAG TACACGTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_954711.1
    CDS55..3276
    Translation

    Target ORF information:

    RefSeq Version NM_199241.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_199241.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG
    GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC
    TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA
    GGTATCCCAA AGGGTGTACG GTGGGAAGTC CAGTCTGGAG AATCCAATCA GATGGTCCAC
    ATGAATGTCC TCATCACCTG CGTGTTTGCC GCTTTTGTCT TGGGCGCGTT CATCGCAGGA
    GTGGCCGTGT ACTGCTACCG TGACATGTTC GTTCGGAAGA ACAGAAAGAT CCATAAAGAC
    GCAGAATCCG CCCAGTCGTG CACAGACTCC AGCGGAAGCT TCGCCAAGCT GAACGGCCTC
    TTTGACAGCC CCGTCAAGGA ATACCAGCAG AACATTGATT CTCCCAAACT CTACAGCAAC
    CTGCTGACCA GTCGGAAGGA ACTGCCACCA AACACGGATA CAAAGTCCAT GGCCGTGGAC
    CACAGAGGCC AGCCTCCCGA GCTGGCTGCT CTCCCCACGC CGGAATCCAC ACCTGTCCTC
    CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCTGAGA AGGCCCACAG CCACGGTGCT
    TCAAGGAAAG AACACCCCCA GTTTTTTCCT TCTAGTCCTC CACCCCATTC CCCATTGAGT
    CACGGGCATA TCCCCAGTGC CATCGTTCTT CCAAACGCCA CTCACGACTA CAATACATCC
    TTCTCCAACT CGAATGCCCA CAAAGCCGAA AAGAAGCTTC AGAGCATGGA TCACCCTCTT
    ACGAAGTCAT CCAGTAAGCG GGAGCACCGG CGGTCTGTGG ATTCCAGGAA TACTCTCAAT
    GATCTCCTGA AGCATCTAAA TGACCCAAAC AGTAACCCCA AAGCCATCCT GGGAGAGATC
    CATATGGCTC ATCAAACCCT CATGCTGGAC CCGGTGGGAC CAATGGCTGA GGTCCCACCC
    AAGGTCCCTA ACCGGGAGGC ATCTCTATAC TCCCCTCCCT CCACACTCCC CAGAAATAGT
    CCAACCAAGA GAGTAGATGT CCCCACCACT CCTGGGGTGC CAATGACTTC TCTGGAAAGA
    CAAAGGGGTT ATCACAAAAA TTCCTCCCAG AGGCACTCTA TATCTGCCGT GCCTAAAAAC
    TTAAACTCAC CAAATGGTGT TTTGTTATCT AGACAGCCGA GTATGAACCG TGGAGGCTAT
    ATGCCCACCC CAACAGGGGC GAAGGTGGAC TATATTCAGG GGACACCGGT GAGTGTTCAT
    CTGCAGCCCT CCCTCTCCAG ACAGAGCAGC TATACCAGTA ATGGCACCCT CCCCAGGACG
    GGACTAAAGA GGACACCATC CTTAAAACCT GATGTGCCAC CAAAGCCTTC CTTTGTTCCG
    CAAACCACAT CTGTCAGACC ACTGAACAAG TACACGTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20528
    Clone ID Related Accession (Same CDS sequence) XM_017317012.2 , XM_030249363.1 , XM_011239427.3 , XM_030249361.1 , XM_017317012.1 , XM_011239427.2 , NM_199240.3
    Accession Version NM_199240.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3165bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-10
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform Sema6D-6 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC060680.1, AB091535.1 and AL935323.6. On Mar 21, 2014 this sequence version replaced NM_199240.2. Transcript Variant: This variant (6) uses an alternate in-frame splice site and contains an additional in-frame exon in the central coding region, compared to variant 1. The encoded isoform (Sema6D-6) is longer than isoform Sema6D-1. The 5' UTR is incomplete because the evidence for this variant is for the CDS only, and multiple possible 5' UTR exon combinations exist. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB091535.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT
    GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA
    TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC
    CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA
    GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA
    GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC
    CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC
    AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG
    GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC
    CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT
    GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG
    AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA
    TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT
    CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC
    AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG
    ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA
    CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT
    AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA
    AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA
    AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC
    TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT
    CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG
    ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT
    CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_954710.1
    CDS55..3219
    Translation

    Target ORF information:

    RefSeq Version NM_199240.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_199240.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT
    GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA
    TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC
    CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA
    GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA
    GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC
    CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC
    AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG
    GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC
    CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT
    GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG
    AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA
    TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT
    CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC
    AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG
    ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA
    CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT
    AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA
    AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA
    AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC
    TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT
    CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG
    ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT
    CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20556
    Clone ID Related Accession (Same CDS sequence) NM_001291000.1 , XM_030249347.1 , XM_030249351.1
    Accession Version NM_001291000.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-10
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform 3 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB091532.1, AK031307.1, AL935323.6 and BC060680.1. On Mar 21, 2014 this sequence version replaced XM_006499117.1. Transcript Variant: This variant (7) uses an alternate in-frame splice site and contains two additional in-frame exons in the central coding region, compared to variant 1. The encoded isoform (3) is longer than isoform Sema6D-1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK031307.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG
    GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC
    TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA
    GGTGTACGGT GGGAAGTCCA GTCTGGAGAA TCCAATCAGA TGGTCCACAT GAATGTCCTC
    ATCACCTGCG TGTTTGCCGC TTTTGTCTTG GGCGCGTTCA TCGCAGGAGT GGCCGTGTAC
    TGCTACCGTG ACATGTTCGT TCGGAAGAAC AGAAAGATCC ATAAAGACGC AGAATCCGCC
    CAGTCGTGCA CAGACTCCAG CGGAAGCTTC GCCAAGCTGA ACGGCCTCTT TGACAGCCCC
    GTCAAGGAAT ACCAGCAGAA CATTGATTCT CCCAAACTCT ACAGCAACCT GCTGACCAGT
    CGGAAGGAAC TGCCACCAAA CACGGATACA AAGTCCATGG CCGTGGACCA CAGAGGCCAG
    CCTCCCGAGC TGGCTGCTCT CCCCACGCCG GAATCCACAC CTGTCCTCCA CCAGAAGACC
    CTGCAGGCCA TGAAGAGCCA CTCTGAGAAG GCCCACAGCC ACGGTGCTTC AAGGAAAGAA
    CACCCCCAGT TTTTTCCTTC TAGTCCTCCA CCCCATTCCC CATTGAGTCA CGGGCATATC
    CCCAGTGCCA TCGTTCTTCC AAACGCCACT CACGACTACA ATACATCCTT CTCCAACTCG
    AATGCCCACA AAGCCGAAAA GAAGCTTCAG AGCATGGATC ACCCTCTTAC GAAGTCATCC
    AGTAAGCGGG AGCACCGGCG GTCTGTGGAT TCCAGGAATA CTCTCAATGA TCTCCTGAAG
    CATCTAAATG ACCCAAACAG TAACCCCAAA GCCATCCTGG GAGAGATCCA TATGGCTCAT
    CAAACCCTCA TGCTGGACCC GGTGGGACCA ATGGCTGAGG TCCCACCCAA GGTCCCTAAC
    CGGGAGGCAT CTCTATACTC CCCTCCCTCC ACACTCCCCA GAAATAGTCC AACCAAGAGA
    GTAGATGTCC CCACCACTCC TGGGGTGCCA ATGACTTCTC TGGAAAGACA AAGGGGTTAT
    CACAAAAATT CCTCCCAGAG GCACTCTATA TCTGCCGTGC CTAAAAACTT AAACTCACCA
    AATGGTGTTT TGTTATCTAG ACAGCCGAGT ATGAACCGTG GAGGCTATAT GCCCACCCCA
    ACAGGGGCGA AGGTGGACTA TATTCAGGGG ACACCGGTGA GTGTTCATCT GCAGCCCTCC
    CTCTCCAGAC AGAGCAGCTA TACCAGTAAT GGCACCCTCC CCAGGACGGG ACTAAAGAGG
    ACACCATCCT TAAAACCTGA TGTGCCACCA AAGCCTTCCT TTGTTCCGCA AACCACATCT
    GTCAGACCAC TGAACAAGTA CACGTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001277929.1
    CDS820..4029
    Translation

    Target ORF information:

    RefSeq Version NM_001291000.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001291000.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG
    GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC
    TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA
    GGTGTACGGT GGGAAGTCCA GTCTGGAGAA TCCAATCAGA TGGTCCACAT GAATGTCCTC
    ATCACCTGCG TGTTTGCCGC TTTTGTCTTG GGCGCGTTCA TCGCAGGAGT GGCCGTGTAC
    TGCTACCGTG ACATGTTCGT TCGGAAGAAC AGAAAGATCC ATAAAGACGC AGAATCCGCC
    CAGTCGTGCA CAGACTCCAG CGGAAGCTTC GCCAAGCTGA ACGGCCTCTT TGACAGCCCC
    GTCAAGGAAT ACCAGCAGAA CATTGATTCT CCCAAACTCT ACAGCAACCT GCTGACCAGT
    CGGAAGGAAC TGCCACCAAA CACGGATACA AAGTCCATGG CCGTGGACCA CAGAGGCCAG
    CCTCCCGAGC TGGCTGCTCT CCCCACGCCG GAATCCACAC CTGTCCTCCA CCAGAAGACC
    CTGCAGGCCA TGAAGAGCCA CTCTGAGAAG GCCCACAGCC ACGGTGCTTC AAGGAAAGAA
    CACCCCCAGT TTTTTCCTTC TAGTCCTCCA CCCCATTCCC CATTGAGTCA CGGGCATATC
    CCCAGTGCCA TCGTTCTTCC AAACGCCACT CACGACTACA ATACATCCTT CTCCAACTCG
    AATGCCCACA AAGCCGAAAA GAAGCTTCAG AGCATGGATC ACCCTCTTAC GAAGTCATCC
    AGTAAGCGGG AGCACCGGCG GTCTGTGGAT TCCAGGAATA CTCTCAATGA TCTCCTGAAG
    CATCTAAATG ACCCAAACAG TAACCCCAAA GCCATCCTGG GAGAGATCCA TATGGCTCAT
    CAAACCCTCA TGCTGGACCC GGTGGGACCA ATGGCTGAGG TCCCACCCAA GGTCCCTAAC
    CGGGAGGCAT CTCTATACTC CCCTCCCTCC ACACTCCCCA GAAATAGTCC AACCAAGAGA
    GTAGATGTCC CCACCACTCC TGGGGTGCCA ATGACTTCTC TGGAAAGACA AAGGGGTTAT
    CACAAAAATT CCTCCCAGAG GCACTCTATA TCTGCCGTGC CTAAAAACTT AAACTCACCA
    AATGGTGTTT TGTTATCTAG ACAGCCGAGT ATGAACCGTG GAGGCTATAT GCCCACCCCA
    ACAGGGGCGA AGGTGGACTA TATTCAGGGG ACACCGGTGA GTGTTCATCT GCAGCCCTCC
    CTCTCCAGAC AGAGCAGCTA TACCAGTAAT GGCACCCTCC CCAGGACGGG ACTAAAGAGG
    ACACCATCCT TAAAACCTGA TGTGCCACCA AAGCCTTCCT TTGTTCCGCA AACCACATCT
    GTCAGACCAC TGAACAAGTA CACGTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20434
    Clone ID Related Accession (Same CDS sequence) XM_017317019.2 , XM_017317019.1 , NM_199239.3
    Accession Version XM_017317019.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3093bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC
    CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG
    TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC
    GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC
    CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC
    AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC
    CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG
    ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA
    GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT
    ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC
    TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA
    TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG
    AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT
    CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT
    AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG
    AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT
    TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA
    CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC
    CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC
    TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG
    AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA
    TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172508.1
    CDS832..3924
    Translation

    Target ORF information:

    RefSeq Version XM_017317019.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317019.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC
    CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG
    TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC
    GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC
    CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC
    AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC
    CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG
    ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA
    GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT
    ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC
    TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA
    TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG
    AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT
    CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT
    AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG
    AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT
    TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA
    CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC
    CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC
    TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG
    AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA
    TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20490
    Clone ID Related Accession (Same CDS sequence) XM_030249343.1 , XM_030249345.1 , XM_017317001.1 , XM_017317001.2 , NM_199241.3
    Accession Version XM_017317001.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG
    GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC
    TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA
    GGTATCCCAA AGGGTGTACG GTGGGAAGTC CAGTCTGGAG AATCCAATCA GATGGTCCAC
    ATGAATGTCC TCATCACCTG CGTGTTTGCC GCTTTTGTCT TGGGCGCGTT CATCGCAGGA
    GTGGCCGTGT ACTGCTACCG TGACATGTTC GTTCGGAAGA ACAGAAAGAT CCATAAAGAC
    GCAGAATCCG CCCAGTCGTG CACAGACTCC AGCGGAAGCT TCGCCAAGCT GAACGGCCTC
    TTTGACAGCC CCGTCAAGGA ATACCAGCAG AACATTGATT CTCCCAAACT CTACAGCAAC
    CTGCTGACCA GTCGGAAGGA ACTGCCACCA AACACGGATA CAAAGTCCAT GGCCGTGGAC
    CACAGAGGCC AGCCTCCCGA GCTGGCTGCT CTCCCCACGC CGGAATCCAC ACCTGTCCTC
    CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCTGAGA AGGCCCACAG CCACGGTGCT
    TCAAGGAAAG AACACCCCCA GTTTTTTCCT TCTAGTCCTC CACCCCATTC CCCATTGAGT
    CACGGGCATA TCCCCAGTGC CATCGTTCTT CCAAACGCCA CTCACGACTA CAATACATCC
    TTCTCCAACT CGAATGCCCA CAAAGCCGAA AAGAAGCTTC AGAGCATGGA TCACCCTCTT
    ACGAAGTCAT CCAGTAAGCG GGAGCACCGG CGGTCTGTGG ATTCCAGGAA TACTCTCAAT
    GATCTCCTGA AGCATCTAAA TGACCCAAAC AGTAACCCCA AAGCCATCCT GGGAGAGATC
    CATATGGCTC ATCAAACCCT CATGCTGGAC CCGGTGGGAC CAATGGCTGA GGTCCCACCC
    AAGGTCCCTA ACCGGGAGGC ATCTCTATAC TCCCCTCCCT CCACACTCCC CAGAAATAGT
    CCAACCAAGA GAGTAGATGT CCCCACCACT CCTGGGGTGC CAATGACTTC TCTGGAAAGA
    CAAAGGGGTT ATCACAAAAA TTCCTCCCAG AGGCACTCTA TATCTGCCGT GCCTAAAAAC
    TTAAACTCAC CAAATGGTGT TTTGTTATCT AGACAGCCGA GTATGAACCG TGGAGGCTAT
    ATGCCCACCC CAACAGGGGC GAAGGTGGAC TATATTCAGG GGACACCGGT GAGTGTTCAT
    CTGCAGCCCT CCCTCTCCAG ACAGAGCAGC TATACCAGTA ATGGCACCCT CCCCAGGACG
    GGACTAAAGA GGACACCATC CTTAAAACCT GATGTGCCAC CAAAGCCTTC CTTTGTTCCG
    CAAACCACAT CTGTCAGACC ACTGAACAAG TACACGTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172490.1
    CDS832..4053
    Translation

    Target ORF information:

    RefSeq Version XM_017317001.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317001.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG
    GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC
    TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA
    GGTATCCCAA AGGGTGTACG GTGGGAAGTC CAGTCTGGAG AATCCAATCA GATGGTCCAC
    ATGAATGTCC TCATCACCTG CGTGTTTGCC GCTTTTGTCT TGGGCGCGTT CATCGCAGGA
    GTGGCCGTGT ACTGCTACCG TGACATGTTC GTTCGGAAGA ACAGAAAGAT CCATAAAGAC
    GCAGAATCCG CCCAGTCGTG CACAGACTCC AGCGGAAGCT TCGCCAAGCT GAACGGCCTC
    TTTGACAGCC CCGTCAAGGA ATACCAGCAG AACATTGATT CTCCCAAACT CTACAGCAAC
    CTGCTGACCA GTCGGAAGGA ACTGCCACCA AACACGGATA CAAAGTCCAT GGCCGTGGAC
    CACAGAGGCC AGCCTCCCGA GCTGGCTGCT CTCCCCACGC CGGAATCCAC ACCTGTCCTC
    CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCTGAGA AGGCCCACAG CCACGGTGCT
    TCAAGGAAAG AACACCCCCA GTTTTTTCCT TCTAGTCCTC CACCCCATTC CCCATTGAGT
    CACGGGCATA TCCCCAGTGC CATCGTTCTT CCAAACGCCA CTCACGACTA CAATACATCC
    TTCTCCAACT CGAATGCCCA CAAAGCCGAA AAGAAGCTTC AGAGCATGGA TCACCCTCTT
    ACGAAGTCAT CCAGTAAGCG GGAGCACCGG CGGTCTGTGG ATTCCAGGAA TACTCTCAAT
    GATCTCCTGA AGCATCTAAA TGACCCAAAC AGTAACCCCA AAGCCATCCT GGGAGAGATC
    CATATGGCTC ATCAAACCCT CATGCTGGAC CCGGTGGGAC CAATGGCTGA GGTCCCACCC
    AAGGTCCCTA ACCGGGAGGC ATCTCTATAC TCCCCTCCCT CCACACTCCC CAGAAATAGT
    CCAACCAAGA GAGTAGATGT CCCCACCACT CCTGGGGTGC CAATGACTTC TCTGGAAAGA
    CAAAGGGGTT ATCACAAAAA TTCCTCCCAG AGGCACTCTA TATCTGCCGT GCCTAAAAAC
    TTAAACTCAC CAAATGGTGT TTTGTTATCT AGACAGCCGA GTATGAACCG TGGAGGCTAT
    ATGCCCACCC CAACAGGGGC GAAGGTGGAC TATATTCAGG GGACACCGGT GAGTGTTCAT
    CTGCAGCCCT CCCTCTCCAG ACAGAGCAGC TATACCAGTA ATGGCACCCT CCCCAGGACG
    GGACTAAAGA GGACACCATC CTTAAAACCT GATGTGCCAC CAAAGCCTTC CTTTGTTCCG
    CAAACCACAT CTGTCAGACC ACTGAACAAG TACACGTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20528
    Clone ID Related Accession (Same CDS sequence) XM_017317012.2 , XM_030249363.1 , XM_011239427.3 , XM_030249361.1 , XM_017317012.1 , XM_011239427.2 , NM_199240.3
    Accession Version XM_017317012.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3165bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT
    GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA
    TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC
    CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA
    GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA
    GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC
    CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC
    AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG
    GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC
    CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT
    GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG
    AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA
    TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT
    CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC
    AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG
    ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA
    CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT
    AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA
    AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA
    AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC
    TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT
    CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG
    ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT
    CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172501.1
    CDS832..3996
    Translation

    Target ORF information:

    RefSeq Version XM_017317012.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317012.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT
    GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA
    TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC
    CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA
    GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA
    GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC
    CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC
    AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG
    GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC
    CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT
    GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG
    AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA
    TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT
    CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC
    AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG
    ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA
    CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT
    AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA
    AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA
    AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC
    TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT
    CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG
    ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT
    CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20528
    Clone ID Related Accession (Same CDS sequence) XM_017317012.2 , XM_030249363.1 , XM_011239427.3 , XM_030249361.1 , XM_017317012.1 , XM_011239427.2 , NM_199240.3
    Accession Version XM_011239427.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3165bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011239427.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT
    GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA
    TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC
    CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA
    GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA
    GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC
    CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC
    AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG
    GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC
    CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT
    GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG
    AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA
    TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT
    CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC
    AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG
    ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA
    CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT
    AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA
    AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA
    AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC
    TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT
    CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG
    ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT
    CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011237729.1
    CDS630..3794
    Translation

    Target ORF information:

    RefSeq Version XM_011239427.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011239427.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT
    GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA
    TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC
    CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA
    GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA
    GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC
    CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC
    AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG
    GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC
    CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT
    GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG
    AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA
    TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT
    CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC
    AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG
    ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA
    CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT
    AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA
    AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA
    AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC
    TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT
    CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG
    ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT
    CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu30226
    Clone ID Related Accession (Same CDS sequence) XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
    Accession Version XM_006499111.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-02-08
    Organism Mus musculus(House mouse)
    Product semaphorin-6D isoform X5
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006499174.1
    Translation

    Target ORF information:

    RefSeq Version XM_006499111.2
    Organism Mus musculus(House mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006499111.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu30226
    Clone ID Related Accession (Same CDS sequence) XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
    Accession Version XM_006499111.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499111.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006499174.1
    CDS832..4092
    Translation

    Target ORF information:

    RefSeq Version XM_006499111.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006499111.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu30226
    Clone ID Related Accession (Same CDS sequence) XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
    Accession Version XM_006499114.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499114.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006499177.1
    CDS859..4119
    Translation

    Target ORF information:

    RefSeq Version XM_006499114.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006499114.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu30226
    Clone ID Related Accession (Same CDS sequence) XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
    Accession Version XM_006499113.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-02-08
    Organism Mus musculus(House mouse)
    Product semaphorin-6D isoform X5
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006499176.1
    Translation

    Target ORF information:

    RefSeq Version XM_006499113.2
    Organism Mus musculus(House mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006499113.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu30226
    Clone ID Related Accession (Same CDS sequence) XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
    Accession Version XM_006499112.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-02-08
    Organism Mus musculus(House mouse)
    Product semaphorin-6D isoform X5
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006499175.1
    Translation

    Target ORF information:

    RefSeq Version XM_006499112.2
    Organism Mus musculus(House mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006499112.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu30226
    Clone ID Related Accession (Same CDS sequence) XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
    Accession Version XM_006499112.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499112.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006499175.1
    CDS369..3629
    Translation

    Target ORF information:

    RefSeq Version XM_006499112.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006499112.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu30226
    Clone ID Related Accession (Same CDS sequence) XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
    Accession Version XM_006499114.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-02-08
    Organism Mus musculus(House mouse)
    Product semaphorin-6D isoform X5
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006499177.1
    Translation

    Target ORF information:

    RefSeq Version XM_006499114.2
    Organism Mus musculus(House mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006499114.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu30226
    Clone ID Related Accession (Same CDS sequence) XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
    Accession Version XM_006499113.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499113.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006499176.1
    CDS438..3698
    Translation

    Target ORF information:

    RefSeq Version XM_006499113.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006499113.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC
    GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT
    GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC
    ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA
    TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA
    CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG
    CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC
    ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC
    AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG
    GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC
    ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA
    TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC
    CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG
    AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA
    CTGAACAAGT ACACGTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu72911
    Clone ID Related Accession (Same CDS sequence) XM_017316995.2 , XM_017316995.1
    Accession Version XM_017316995.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTGTACGGTG GGAAGTCCAG
    TCTGGAGAAT CCAATCAGAT GGTCCACATG AATGTCCTCA TCACCTGCGT GTTTGCCGCT
    TTTGTCTTGG GCGCGTTCAT CGCAGGAGTG GCCGTGTACT GCTACCGTGA CATGTTCGTT
    CGGAAGAACA GAAAGATCCA TAAAGACGCA GAATCCGCCC AGTCGTGCAC AGACTCCAGC
    GGAAGCTTCG CCAAGCTGAA CGGCCTCTTT GACAGCCCCG TCAAGGAATA CCAGCAGAAC
    ATTGATTCTC CCAAACTCTA CAGCAACCTG CTGACCAGTC GGAAGGAACT GCCACCAAAC
    ACGGATACAA AGTCCATGGC CGTGGACCAC AGAGGCCAGC CTCCCGAGCT GGCTGCTCTC
    CCCACGCCGG AATCCACACC TGTCCTCCAC CAGAAGACCC TGCAGGCCAT GAAGAGCCAC
    TCTGAGAAGG CCCACAGCCA CGGTGCTTCA AGGAAAGAAC ACCCCCAGTT TTTTCCTTCT
    AGTCCTCCAC CCCATTCCCC ATTGAGTCAC GGGCATATCC CCAGTGCCAT CGTTCTTCCA
    AACGCCACTC ACGACTACAA TACATCCTTC TCCAACTCGA ATGCCCACAA AGCCGAAAAG
    AAGCTTCAGA GCATGGATCA CCCTCTTACG AAGTCATCCA GTAAGCGGGA GCACCGGCGG
    TCTGTGGATT CCAGGAATAC TCTCAATGAT CTCCTGAAGC ATCTAAATGA CCCAAACAGT
    AACCCCAAAG CCATCCTGGG AGAGATCCAT ATGGCTCATC AAACCCTCAT GCTGGACCCG
    GTGGGACCAA TGGCTGAGGT CCCACCCAAG GTCCCTAACC GGGAGGCATC TCTATACTCC
    CCTCCCTCCA CACTCCCCAG AAATAGTCCA ACCAAGAGAG TAGATGTCCC CACCACTCCT
    GGGGTGCCAA TGACTTCTCT GGAAAGACAA AGGGGTTATC ACAAAAATTC CTCCCAGAGG
    CACTCTATAT CTGCCGTGCC TAAAAACTTA AACTCACCAA ATGGTGTTTT GTTATCTAGA
    CAGCCGAGTA TGAACCGTGG AGGCTATATG CCCACCCCAA CAGGGGCGAA GGTGGACTAT
    ATTCAGGGGA CACCGGTGAG TGTTCATCTG CAGCCCTCCC TCTCCAGACA GAGCAGCTAT
    ACCAGTAATG GCACCCTCCC CAGGACGGGA CTAAAGAGGA CACCATCCTT AAAACCTGAT
    GTGCCACCAA AGCCTTCCTT TGTTCCGCAA ACCACATCTG TCAGACCACT GAACAAGTAC
    ACGTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172484.1
    CDS832..4080
    Translation

    Target ORF information:

    RefSeq Version XM_017316995.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017316995.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA
    TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTGTACGGTG GGAAGTCCAG
    TCTGGAGAAT CCAATCAGAT GGTCCACATG AATGTCCTCA TCACCTGCGT GTTTGCCGCT
    TTTGTCTTGG GCGCGTTCAT CGCAGGAGTG GCCGTGTACT GCTACCGTGA CATGTTCGTT
    CGGAAGAACA GAAAGATCCA TAAAGACGCA GAATCCGCCC AGTCGTGCAC AGACTCCAGC
    GGAAGCTTCG CCAAGCTGAA CGGCCTCTTT GACAGCCCCG TCAAGGAATA CCAGCAGAAC
    ATTGATTCTC CCAAACTCTA CAGCAACCTG CTGACCAGTC GGAAGGAACT GCCACCAAAC
    ACGGATACAA AGTCCATGGC CGTGGACCAC AGAGGCCAGC CTCCCGAGCT GGCTGCTCTC
    CCCACGCCGG AATCCACACC TGTCCTCCAC CAGAAGACCC TGCAGGCCAT GAAGAGCCAC
    TCTGAGAAGG CCCACAGCCA CGGTGCTTCA AGGAAAGAAC ACCCCCAGTT TTTTCCTTCT
    AGTCCTCCAC CCCATTCCCC ATTGAGTCAC GGGCATATCC CCAGTGCCAT CGTTCTTCCA
    AACGCCACTC ACGACTACAA TACATCCTTC TCCAACTCGA ATGCCCACAA AGCCGAAAAG
    AAGCTTCAGA GCATGGATCA CCCTCTTACG AAGTCATCCA GTAAGCGGGA GCACCGGCGG
    TCTGTGGATT CCAGGAATAC TCTCAATGAT CTCCTGAAGC ATCTAAATGA CCCAAACAGT
    AACCCCAAAG CCATCCTGGG AGAGATCCAT ATGGCTCATC AAACCCTCAT GCTGGACCCG
    GTGGGACCAA TGGCTGAGGT CCCACCCAAG GTCCCTAACC GGGAGGCATC TCTATACTCC
    CCTCCCTCCA CACTCCCCAG AAATAGTCCA ACCAAGAGAG TAGATGTCCC CACCACTCCT
    GGGGTGCCAA TGACTTCTCT GGAAAGACAA AGGGGTTATC ACAAAAATTC CTCCCAGAGG
    CACTCTATAT CTGCCGTGCC TAAAAACTTA AACTCACCAA ATGGTGTTTT GTTATCTAGA
    CAGCCGAGTA TGAACCGTGG AGGCTATATG CCCACCCCAA CAGGGGCGAA GGTGGACTAT
    ATTCAGGGGA CACCGGTGAG TGTTCATCTG CAGCCCTCCC TCTCCAGACA GAGCAGCTAT
    ACCAGTAATG GCACCCTCCC CAGGACGGGA CTAAAGAGGA CACCATCCTT AAAACCTGAT
    GTGCCACCAA AGCCTTCCTT TGTTCCGCAA ACCACATCTG TCAGACCACT GAACAAGTAC
    ACGTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu72912
    Clone ID Related Accession (Same CDS sequence) XM_030249357.1 , XM_017317010.1 , XM_017317010.2
    Accession Version XM_017317010.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3204bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGACA TGGAGGTATC CTCATCTTCT GTTACCACTG TGGCAAGTAG CCCAGAAATT
    ACATCTAAAG TGATTGATAC CTGGAGACCT AAACTGACGA GCTCCCGGAA ATTTGTAGTT
    CAAGATGACC CAAATACTTC TGATTTTACT GATACTATAT CAGGTATCCC AAAGGGTGTA
    CGGTGGGAAG TCCAGTCTGG AGAATCCAAT CAGATGGTCC ACATGAATGT CCTCATCACC
    TGCGTGTTTG CCGCTTTTGT CTTGGGCGCG TTCATCGCAG GAGTGGCCGT GTACTGCTAC
    CGTGACATGT TCGTTCGGAA GAACAGAAAG ATCCATAAAG ACGCAGAATC CGCCCAGTCG
    TGCACAGACT CCAGCGGAAG CTTCGCCAAG CTGAACGGCC TCTTTGACAG CCCCGTCAAG
    GAATACCAGC AGAACATTGA TTCTCCCAAA CTCTACAGCA ACCTGCTGAC CAGTCGGAAG
    GAACTGCCAC CAAACACGGA TACAAAGTCC ATGGCCGTGG ACCACAGAGG CCAGCCTCCC
    GAGCTGGCTG CTCTCCCCAC GCCGGAATCC ACACCTGTCC TCCACCAGAA GACCCTGCAG
    GCCATGAAGA GCCACTCTGA GAAGGCCCAC AGCCACGGTG CTTCAAGGAA AGAACACCCC
    CAGTTTTTTC CTTCTAGTCC TCCACCCCAT TCCCCATTGA GTCACGGGCA TATCCCCAGT
    GCCATCGTTC TTCCAAACGC CACTCACGAC TACAATACAT CCTTCTCCAA CTCGAATGCC
    CACAAAGCCG AAAAGAAGCT TCAGAGCATG GATCACCCTC TTACGAAGTC ATCCAGTAAG
    CGGGAGCACC GGCGGTCTGT GGATTCCAGG AATACTCTCA ATGATCTCCT GAAGCATCTA
    AATGACCCAA ACAGTAACCC CAAAGCCATC CTGGGAGAGA TCCATATGGC TCATCAAACC
    CTCATGCTGG ACCCGGTGGG ACCAATGGCT GAGGTCCCAC CCAAGGTCCC TAACCGGGAG
    GCATCTCTAT ACTCCCCTCC CTCCACACTC CCCAGAAATA GTCCAACCAA GAGAGTAGAT
    GTCCCCACCA CTCCTGGGGT GCCAATGACT TCTCTGGAAA GACAAAGGGG TTATCACAAA
    AATTCCTCCC AGAGGCACTC TATATCTGCC GTGCCTAAAA ACTTAAACTC ACCAAATGGT
    GTTTTGTTAT CTAGACAGCC GAGTATGAAC CGTGGAGGCT ATATGCCCAC CCCAACAGGG
    GCGAAGGTGG ACTATATTCA GGGGACACCG GTGAGTGTTC ATCTGCAGCC CTCCCTCTCC
    AGACAGAGCA GCTATACCAG TAATGGCACC CTCCCCAGGA CGGGACTAAA GAGGACACCA
    TCCTTAAAAC CTGATGTGCC ACCAAAGCCT TCCTTTGTTC CGCAAACCAC ATCTGTCAGA
    CCACTGAACA AGTACACGTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172499.1
    CDS832..4035
    Translation

    Target ORF information:

    RefSeq Version XM_017317010.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317010.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC
    TGCCACGACA TGGAGGTATC CTCATCTTCT GTTACCACTG TGGCAAGTAG CCCAGAAATT
    ACATCTAAAG TGATTGATAC CTGGAGACCT AAACTGACGA GCTCCCGGAA ATTTGTAGTT
    CAAGATGACC CAAATACTTC TGATTTTACT GATACTATAT CAGGTATCCC AAAGGGTGTA
    CGGTGGGAAG TCCAGTCTGG AGAATCCAAT CAGATGGTCC ACATGAATGT CCTCATCACC
    TGCGTGTTTG CCGCTTTTGT CTTGGGCGCG TTCATCGCAG GAGTGGCCGT GTACTGCTAC
    CGTGACATGT TCGTTCGGAA GAACAGAAAG ATCCATAAAG ACGCAGAATC CGCCCAGTCG
    TGCACAGACT CCAGCGGAAG CTTCGCCAAG CTGAACGGCC TCTTTGACAG CCCCGTCAAG
    GAATACCAGC AGAACATTGA TTCTCCCAAA CTCTACAGCA ACCTGCTGAC CAGTCGGAAG
    GAACTGCCAC CAAACACGGA TACAAAGTCC ATGGCCGTGG ACCACAGAGG CCAGCCTCCC
    GAGCTGGCTG CTCTCCCCAC GCCGGAATCC ACACCTGTCC TCCACCAGAA GACCCTGCAG
    GCCATGAAGA GCCACTCTGA GAAGGCCCAC AGCCACGGTG CTTCAAGGAA AGAACACCCC
    CAGTTTTTTC CTTCTAGTCC TCCACCCCAT TCCCCATTGA GTCACGGGCA TATCCCCAGT
    GCCATCGTTC TTCCAAACGC CACTCACGAC TACAATACAT CCTTCTCCAA CTCGAATGCC
    CACAAAGCCG AAAAGAAGCT TCAGAGCATG GATCACCCTC TTACGAAGTC ATCCAGTAAG
    CGGGAGCACC GGCGGTCTGT GGATTCCAGG AATACTCTCA ATGATCTCCT GAAGCATCTA
    AATGACCCAA ACAGTAACCC CAAAGCCATC CTGGGAGAGA TCCATATGGC TCATCAAACC
    CTCATGCTGG ACCCGGTGGG ACCAATGGCT GAGGTCCCAC CCAAGGTCCC TAACCGGGAG
    GCATCTCTAT ACTCCCCTCC CTCCACACTC CCCAGAAATA GTCCAACCAA GAGAGTAGAT
    GTCCCCACCA CTCCTGGGGT GCCAATGACT TCTCTGGAAA GACAAAGGGG TTATCACAAA
    AATTCCTCCC AGAGGCACTC TATATCTGCC GTGCCTAAAA ACTTAAACTC ACCAAATGGT
    GTTTTGTTAT CTAGACAGCC GAGTATGAAC CGTGGAGGCT ATATGCCCAC CCCAACAGGG
    GCGAAGGTGG ACTATATTCA GGGGACACCG GTGAGTGTTC ATCTGCAGCC CTCCCTCTCC
    AGACAGAGCA GCTATACCAG TAATGGCACC CTCCCCAGGA CGGGACTAAA GAGGACACCA
    TCCTTAAAAC CTGATGTGCC ACCAAAGCCT TCCTTTGTTC CGCAAACCAC ATCTGTCAGA
    CCACTGAACA AGTACACGTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu72913
    Clone ID Related Accession (Same CDS sequence) XM_017317021.2 , XM_030249368.1 , XM_017317021.1
    Accession Version XM_017317021.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3054bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product semaphorin-6D isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGGTGTA CGGTGGGAAG TCCAGTCTGG AGAATCCAAT
    CAGATGGTCC ACATGAATGT CCTCATCACC TGCGTGTTTG CCGCTTTTGT CTTGGGCGCG
    TTCATCGCAG GAGTGGCCGT GTACTGCTAC CGTGACATGT TCGTTCGGAA GAACAGAAAG
    ATCCATAAAG ACGCAGAATC CGCCCAGTCG TGCACAGACT CCAGCGGAAG CTTCGCCAAG
    CTGAACGGCC TCTTTGACAG CCCCGTCAAG GAATACCAGC AGAACATTGA TTCTCCCAAA
    CTCTACAGCA ACCTGCTGAC CAGTCGGAAG GAACTGCCAC CAAACACGGA TACAAAGTCC
    ATGGCCGTGG ACCACAGAGG CCAGCCTCCC GAGCTGGCTG CTCTCCCCAC GCCGGAATCC
    ACACCTGTCC TCCACCAGAA GACCCTGCAG GCCATGAAGA GCCACTCTGA GAAGGCCCAC
    AGCCACGGTG CTTCAAGGAA AGAACACCCC CAGTTTTTTC CTTCTAGTCC TCCACCCCAT
    TCCCCATTGA GTCACGGGCA TATCCCCAGT GCCATCGTTC TTCCAAACGC CACTCACGAC
    TACAATACAT CCTTCTCCAA CTCGAATGCC CACAAAGCCG AAAAGAAGCT TCAGAGCATG
    GATCACCCTC TTACGAAGTC ATCCAGTAAG CGGGAGCACC GGCGGTCTGT GGATTCCAGG
    AATACTCTCA ATGATCTCCT GAAGCATCTA AATGACCCAA ACAGTAACCC CAAAGCCATC
    CTGGGAGAGA TCCATATGGC TCATCAAACC CTCATGCTGG ACCCGGTGGG ACCAATGGCT
    GAGGTCCCAC CCAAGGTCCC TAACCGGGAG GCATCTCTAT ACTCCCCTCC CTCCACACTC
    CCCAGAAATA GTCCAACCAA GAGAGTAGAT GTCCCCACCA CTCCTGGGGT GCCAATGACT
    TCTCTGGAAA GACAAAGGGG TTATCACAAA AATTCCTCCC AGAGGCACTC TATATCTGCC
    GTGCCTAAAA ACTTAAACTC ACCAAATGGT GTTTTGTTAT CTAGACAGCC GAGTATGAAC
    CGTGGAGGCT ATATGCCCAC CCCAACAGGG GCGAAGGTGG ACTATATTCA GGGGACACCG
    GTGAGTGTTC ATCTGCAGCC CTCCCTCTCC AGACAGAGCA GCTATACCAG TAATGGCACC
    CTCCCCAGGA CGGGACTAAA GAGGACACCA TCCTTAAAAC CTGATGTGCC ACCAAAGCCT
    TCCTTTGTTC CGCAAACCAC ATCTGTCAGA CCACTGAACA AGTACACGTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172510.1
    CDS832..3885
    Translation

    Target ORF information:

    RefSeq Version XM_017317021.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317021.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
    GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG
    AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC
    AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT
    TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT
    AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT
    GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA
    AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA
    CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC
    GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA
    AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT
    CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA
    ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC
    AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC
    AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG
    ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC
    TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG
    TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG
    AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG
    AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC
    GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG
    TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT
    TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC
    AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGGTGTA CGGTGGGAAG TCCAGTCTGG AGAATCCAAT
    CAGATGGTCC ACATGAATGT CCTCATCACC TGCGTGTTTG CCGCTTTTGT CTTGGGCGCG
    TTCATCGCAG GAGTGGCCGT GTACTGCTAC CGTGACATGT TCGTTCGGAA GAACAGAAAG
    ATCCATAAAG ACGCAGAATC CGCCCAGTCG TGCACAGACT CCAGCGGAAG CTTCGCCAAG
    CTGAACGGCC TCTTTGACAG CCCCGTCAAG GAATACCAGC AGAACATTGA TTCTCCCAAA
    CTCTACAGCA ACCTGCTGAC CAGTCGGAAG GAACTGCCAC CAAACACGGA TACAAAGTCC
    ATGGCCGTGG ACCACAGAGG CCAGCCTCCC GAGCTGGCTG CTCTCCCCAC GCCGGAATCC
    ACACCTGTCC TCCACCAGAA GACCCTGCAG GCCATGAAGA GCCACTCTGA GAAGGCCCAC
    AGCCACGGTG CTTCAAGGAA AGAACACCCC CAGTTTTTTC CTTCTAGTCC TCCACCCCAT
    TCCCCATTGA GTCACGGGCA TATCCCCAGT GCCATCGTTC TTCCAAACGC CACTCACGAC
    TACAATACAT CCTTCTCCAA CTCGAATGCC CACAAAGCCG AAAAGAAGCT TCAGAGCATG
    GATCACCCTC TTACGAAGTC ATCCAGTAAG CGGGAGCACC GGCGGTCTGT GGATTCCAGG
    AATACTCTCA ATGATCTCCT GAAGCATCTA AATGACCCAA ACAGTAACCC CAAAGCCATC
    CTGGGAGAGA TCCATATGGC TCATCAAACC CTCATGCTGG ACCCGGTGGG ACCAATGGCT
    GAGGTCCCAC CCAAGGTCCC TAACCGGGAG GCATCTCTAT ACTCCCCTCC CTCCACACTC
    CCCAGAAATA GTCCAACCAA GAGAGTAGAT GTCCCCACCA CTCCTGGGGT GCCAATGACT
    TCTCTGGAAA GACAAAGGGG TTATCACAAA AATTCCTCCC AGAGGCACTC TATATCTGCC
    GTGCCTAAAA ACTTAAACTC ACCAAATGGT GTTTTGTTAT CTAGACAGCC GAGTATGAAC
    CGTGGAGGCT ATATGCCCAC CCCAACAGGG GCGAAGGTGG ACTATATTCA GGGGACACCG
    GTGAGTGTTC ATCTGCAGCC CTCCCTCTCC AGACAGAGCA GCTATACCAG TAATGGCACC
    CTCCCCAGGA CGGGACTAAA GAGGACACCA TCCTTAAAAC CTGATGTGCC ACCAAAGCCT
    TCCTTTGTTC CGCAAACCAC ATCTGTCAGA CCACTGAACA AGTACACGTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.