Sema6d cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sema6d
Entrez Gene ID	214968
Full Name	sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D
Synonyms	1110067B02Rik,AA409156,D330011G23,mKIAA1479
General protein information	Preferred Names sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D Names semaphorin-6D
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 F1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006499111.2	XP_006499174.1	semaphorin-6D isoform X5
XM_006499111.3	XP_006499174.1	semaphorin-6D isoform X1
XM_006499114.3	XP_006499177.1	semaphorin-6D isoform X1
XM_011239430.1	XP_011237732.1	semaphorin-6D isoform X4
NM_199238.3	NP_954708.1	semaphorin-6D isoform Sema6D-2 precursor
NM_001291000.1	NP_001277929.1	semaphorin-6D isoform 3 precursor
XM_030249335.1	XP_030105195.1	semaphorin-6D isoform X1
XM_030249357.1	XP_030105217.1	semaphorin-6D isoform X5
XM_017317012.2	XP_017172501.1	semaphorin-6D isoform X7
XM_030249369.1	XP_030105229.1	semaphorin-6D isoform X11
XM_006499113.2	XP_006499176.1	semaphorin-6D isoform X5
XM_017317014.1	XP_017172503.1	semaphorin-6D isoform X6
XM_017316995.2	XP_017172484.1	semaphorin-6D isoform X2
XM_030249343.1	XP_030105203.1	semaphorin-6D isoform X3
XM_030249345.1	XP_030105205.1	semaphorin-6D isoform X3
XM_030249363.1	XP_030105223.1	semaphorin-6D isoform X7
XM_017317014.2	XP_017172503.1	semaphorin-6D isoform X8
XM_017317019.2	XP_017172508.1	semaphorin-6D isoform X9
XM_017317021.2	XP_017172510.1	semaphorin-6D isoform X10
XM_030249368.1	XP_030105228.1	semaphorin-6D isoform X10
XM_030249372.1	XP_030105232.1	semaphorin-6D isoform X12
XM_006499112.2	XP_006499175.1	semaphorin-6D isoform X5
XM_006499112.3	XP_006499175.1	semaphorin-6D isoform X1
XM_017317001.1	XP_017172490.1	semaphorin-6D isoform X3
XM_017317010.1	XP_017172499.1	semaphorin-6D isoform X4
XM_017317019.1	XP_017172508.1	semaphorin-6D isoform X7
XM_006499111.4	XP_006499174.1	semaphorin-6D isoform X1
XM_006499112.4	XP_006499175.1	semaphorin-6D isoform X1
XM_030249334.1	XP_030105194.1	semaphorin-6D isoform X1
XM_006499113.4	XP_006499176.1	semaphorin-6D isoform X1
XM_006499114.4	XP_006499177.1	semaphorin-6D isoform X1
XM_017317001.2	XP_017172490.1	semaphorin-6D isoform X3
XM_030249347.1	XP_030105207.1	semaphorin-6D isoform X4
XM_030249351.1	XP_030105211.1	semaphorin-6D isoform X4
XM_017317010.2	XP_017172499.1	semaphorin-6D isoform X5
XM_030249358.1	XP_030105218.1	semaphorin-6D isoform X6
XM_011239427.3	XP_011237729.1	semaphorin-6D isoform X7
XM_006499114.2	XP_006499177.1	semaphorin-6D isoform X5
XM_006499113.3	XP_006499176.1	semaphorin-6D isoform X1
XM_017317021.1	XP_017172510.1	semaphorin-6D isoform X8
XM_030249361.1	XP_030105221.1	semaphorin-6D isoform X7
XM_030249365.1	XP_030105225.1	semaphorin-6D isoform X8
XM_030249366.1	XP_030105226.1	semaphorin-6D isoform X8
XM_030249371.1	XP_030105231.1	semaphorin-6D isoform X11
XM_030249373.1	XP_030105233.1	semaphorin-6D isoform X12
XM_017316995.1	XP_017172484.1	semaphorin-6D isoform X2
XM_017317012.1	XP_017172501.1	semaphorin-6D isoform X5
XM_011239427.2	XP_011237729.1	semaphorin-6D isoform X5
XM_017317026.1	XP_017172515.1	semaphorin-6D isoform X13
XM_017317028.1	XP_017172517.1	semaphorin-6D isoform X14
NM_199241.3	NP_954711.1	semaphorin-6D isoform Sema6D-4 precursor
NM_172537.4	NP_766125.2	semaphorin-6D isoform Sema6D-1 precursor
NM_001290997.1	NP_001277926.1	semaphorin-6D isoform Sema6D-2 precursor
NM_199239.3	NP_954709.1	semaphorin-6D isoform Sema6D-5 precursor
NM_199240.3	NP_954710.1	semaphorin-6D isoform Sema6D-6 precursor

Pathways from BioSystems

Homologies

	SEMA6D	XP_001112236.1
Canis lupus familiaris (dog)	SEMA6D	XP_005638436.1
Bos taurus (cattle)	SEMA6D	NP_001178062.1
Homo sapiens (human)	SEMA6D	NP_705871.1
Pan troglodytes (chimpanzee)	SEMA6D	XP_003314766.1
Danio rerio (zebrafish)	sema6dl	NP_001017682.2
Mus musculus (house mouse)	Sema6d	NP_954711.1
Rattus norvegicus (Norway rat)	Sema6d	NP_001101238.1
Gallus gallus (chicken)	SEMA6D	XP_001231859.2
Xenopus tropicalis (tropical clawed frog)	sema6d	NP_001120152.1

Gene Ontology
Bibliography

Related articles in PubMed

Transcription and Signaling Regulators in Developing Neuronal Subtypes of Mouse and Human Enteric Nervous System.
Memic F, Knoflach V, Morarach K, Sadler R, Laranjeira C, Hjerling-Leffler J, Sundstr?m E, Pachnis V, Marklund U
Gastroenterology(2017 Oct)

Control of species-dependent cortico-motoneuronal connections underlying manual dexterity.
Gu Z, Kalambogias J, Yoshioka S, Han W, Li Z, Kawasawa YI, Pochareddy S, Li Z, Liu F, Xu X, Wijeratne HRS, Ueno M, Blatz E, Salomone J, Kumanogoh A, Rasin MR, Gebelein B, Weirauch MT, Sestan N, Martin JH, Yoshida Y
Science (New York, N.Y.)357(6349)400-404(2017 07)

Shear stress-regulated miR-27b controls pericyte recruitment by repressing SEMA6A and SEMA6D.
Demolli S, Doddaballapur A, Devraj K, Stark K, Manavski Y, Eckart A, Zehendner CM, Lucas T, Korff T, Hecker M, Massberg S, Liebner S, Kaluza D, Boon RA, Dimmeler S
Cardiovascular research113(6)681-691(2017 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

BMP-SEMA6D-Rho axis plays a key role in the initial stages of AV cushion mesenchyme formation.
Title: Sema6D acts downstream of bone morphogenetic protein signalling to promote atrioventricular cushion development in mice.

Class 6 transmembrane semaphorins are unlikely to serve as major PlexA receptor ligands for the assembly of murine retinal circuit laminar organization.
Title: Sema6B, Sema6C, and Sema6D expression and function during mammalian retinal development.

These findings suggest a mechanism by which a complex of Sema6D, Nr-CAM, and Plexin-A1 at the chiasm midline alters the sign of Sema6D and signals Nr-CAM/Plexin-A1 receptors on retinal ganglion cells to implement the contralateral retinal cell projection.
Title: Optic chiasm presentation of Semaphorin6D in the context of Plexin-A1 and Nr-CAM promotes retinal axon midline crossing.

A mouse null mutation of Sema6d results in ectopic placement of the shafts of proprioceptive axons and their associated oligodendrocytes in the superficial dorsal horn, disrupting its synaptic organization.
Title: Ectopic myelinating oligodendrocytes in the dorsal spinal cord as a consequence of altered semaphorin 6D signaling inhibit synapse formation.

This work reveals a role for semaphorin 6D as a regulator of the late phase of primary immune responses.
Title: Semaphorin 6D regulates the late phase of CD4+ T cell primary immune responses.

The following Sema6d gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sema6d cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu72914	XM_017317026.1 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu72915	XM_017317028.1 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu20440	NM_199238.3 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu20440	NM_001290997.1 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu20440	XM_030249372.1 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu20440	XM_011239430.1 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu20440	XM_030249373.1 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu20434	XM_017317019.2 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu20449	XM_030249371.1 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu20449	XM_030249369.1 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu20490	XM_017317001.2 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu20490	XM_030249343.1 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu20490	XM_030249345.1 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu20528	XM_017317012.2 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu20528	XM_030249361.1 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu20528	XM_011239427.3 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu20528	XM_030249363.1 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu20556	XM_030249347.1 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu20556	XM_030249351.1 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu30226	XM_006499111.4 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu30226	XM_006499114.4 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu30226	XM_006499113.4 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu30226	XM_006499112.4 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu30226	XM_030249334.1 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu30226	XM_030249335.1 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu72911	XM_017316995.2 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu72912	XM_017317010.2 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu72912	XM_030249357.1 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu72913	XM_017317021.2 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu72913	XM_030249368.1 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu80174	XM_017317014.2 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu80174	XM_030249366.1 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu80174	XM_030249365.1 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu87775	XM_030249358.1 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu20434	NM_199239.3 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu20449	NM_172537.4 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$594.30 $849.00
OMu20490	NM_199241.3 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu20528	NM_199240.3 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu20556	NM_001291000.1 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu30226	XM_006499111.2	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu80174	XM_017317014.1	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu30226	XM_006499112.2	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu20434	XM_017317019.1	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu30226	XM_006499111.3	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu30226	XM_006499112.3	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu30226	XM_006499113.2	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu30226	XM_006499114.3	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu20490	XM_017317001.1	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu72912	XM_017317010.1	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu30226	XM_006499114.2	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu30226	XM_006499113.3	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu72913	XM_017317021.1	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu72911	XM_017316995.1	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu20528	XM_017317012.1	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu20528	XM_011239427.2	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sema6d ( XM_017317026.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172515.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X30, mRNA.

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CloneID	OMu72914
Clone ID Related Accession (Same CDS sequence)	XM_017317026.1
Accession Version	XM_017317026.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG CAGCAACTCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172515.1
CDS	832..2805
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISAATL

Target ORF information:

RefSeq Version	XM_017317026.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317026.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG CAGCAACTCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_017317028.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172517.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X31, mRNA.

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CloneID	OMu72915
Clone ID Related Accession (Same CDS sequence)	XM_017317028.1
Accession Version	XM_017317028.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG CAACTCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172517.1
CDS	832..2802
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISATL

Target ORF information:

RefSeq Version	XM_017317028.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317028.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG CAACTCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( NM_199238.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_954708.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 2, mRNA.

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CloneID	OMu20440
Clone ID Related Accession (Same CDS sequence)	XM_011239430.1 , NM_199238.3 , XM_030249372.1 , XM_030249373.1 , NM_001290997.1
Accession Version	NM_199238.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-10
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform Sema6D-2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB091532.1, BC060680.1 and AL935323.6. On Mar 21, 2014 this sequence version replaced NM_199238.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (Sema6D-2) that is shorter than isoform Sema6D-1. Both variants 2 and 3 encode isoform Sema6D-2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC060680.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAATCC 
AATCAGATGG TCCACATGAA TGTCCTCATC ACCTGCGTGT TTGCCGCTTT TGTCTTGGGC 
GCGTTCATCG CAGGAGTGGC CGTGTACTGC TACCGTGACA TGTTCGTTCG GAAGAACAGA 
AAGATCCATA AAGACGCAGA ATCCGCCCAG TCGTGCACAG ACTCCAGCGG AAGCTTCGCC 
AAGCTGAACG GCCTCTTTGA CAGCCCCGTC AAGGAATACC AGCAGAACAT TGATTCTCCC 
AAACTCTACA GCAACCTGCT GACCAGTCGG AAGGAACTGC CACCAAACAC GGATACAAAG 
TCCATGGCCG TGGACCACAG AGGCCAGCCT CCCGAGCTGG CTGCTCTCCC CACGCCGGAA 
TCCACACCTG TCCTCCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC TGAGAAGGCC 
CACAGCCACG GTGCTTCAAG GAAAGAACAC CCCCAGTTTT TTCCTTCTAG TCCTCCACCC 
CATTCCCCAT TGAGTCACGG GCATATCCCC AGTGCCATCG TTCTTCCAAA CGCCACTCAC 
GACTACAATA CATCCTTCTC CAACTCGAAT GCCCACAAAG CCGAAAAGAA GCTTCAGAGC 
ATGGATCACC CTCTTACGAA GTCATCCAGT AAGCGGGAGC ACCGGCGGTC TGTGGATTCC 
AGGAATACTC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC 
ATCCTGGGAG AGATCCATAT GGCTCATCAA ACCCTCATGC TGGACCCGGT GGGACCAATG 
GCTGAGGTCC CACCCAAGGT CCCTAACCGG GAGGCATCTC TATACTCCCC TCCCTCCACA 
CTCCCCAGAA ATAGTCCAAC CAAGAGAGTA GATGTCCCCA CCACTCCTGG GGTGCCAATG 
ACTTCTCTGG AAAGACAAAG GGGTTATCAC AAAAATTCCT CCCAGAGGCA CTCTATATCT 
GCCGTGCCTA AAAACTTAAA CTCACCAAAT GGTGTTTTGT TATCTAGACA GCCGAGTATG 
AACCGTGGAG GCTATATGCC CACCCCAACA GGGGCGAAGG TGGACTATAT TCAGGGGACA 
CCGGTGAGTG TTCATCTGCA GCCCTCCCTC TCCAGACAGA GCAGCTATAC CAGTAATGGC 
ACCCTCCCCA GGACGGGACT AAAGAGGACA CCATCCTTAA AACCTGATGT GCCACCAAAG 
CCTTCCTTTG TTCCGCAAAC CACATCTGTC AGACCACTGA ACAAGTACAC GTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954708.1
CDS	820..3816
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	NM_199238.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199238.3

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAATCC 
AATCAGATGG TCCACATGAA TGTCCTCATC ACCTGCGTGT TTGCCGCTTT TGTCTTGGGC 
GCGTTCATCG CAGGAGTGGC CGTGTACTGC TACCGTGACA TGTTCGTTCG GAAGAACAGA 
AAGATCCATA AAGACGCAGA ATCCGCCCAG TCGTGCACAG ACTCCAGCGG AAGCTTCGCC 
AAGCTGAACG GCCTCTTTGA CAGCCCCGTC AAGGAATACC AGCAGAACAT TGATTCTCCC 
AAACTCTACA GCAACCTGCT GACCAGTCGG AAGGAACTGC CACCAAACAC GGATACAAAG 
TCCATGGCCG TGGACCACAG AGGCCAGCCT CCCGAGCTGG CTGCTCTCCC CACGCCGGAA 
TCCACACCTG TCCTCCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC TGAGAAGGCC 
CACAGCCACG GTGCTTCAAG GAAAGAACAC CCCCAGTTTT TTCCTTCTAG TCCTCCACCC 
CATTCCCCAT TGAGTCACGG GCATATCCCC AGTGCCATCG TTCTTCCAAA CGCCACTCAC 
GACTACAATA CATCCTTCTC CAACTCGAAT GCCCACAAAG CCGAAAAGAA GCTTCAGAGC 
ATGGATCACC CTCTTACGAA GTCATCCAGT AAGCGGGAGC ACCGGCGGTC TGTGGATTCC 
AGGAATACTC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC 
ATCCTGGGAG AGATCCATAT GGCTCATCAA ACCCTCATGC TGGACCCGGT GGGACCAATG 
GCTGAGGTCC CACCCAAGGT CCCTAACCGG GAGGCATCTC TATACTCCCC TCCCTCCACA 
CTCCCCAGAA ATAGTCCAAC CAAGAGAGTA GATGTCCCCA CCACTCCTGG GGTGCCAATG 
ACTTCTCTGG AAAGACAAAG GGGTTATCAC AAAAATTCCT CCCAGAGGCA CTCTATATCT 
GCCGTGCCTA AAAACTTAAA CTCACCAAAT GGTGTTTTGT TATCTAGACA GCCGAGTATG 
AACCGTGGAG GCTATATGCC CACCCCAACA GGGGCGAAGG TGGACTATAT TCAGGGGACA 
CCGGTGAGTG TTCATCTGCA GCCCTCCCTC TCCAGACAGA GCAGCTATAC CAGTAATGGC 
ACCCTCCCCA GGACGGGACT AAAGAGGACA CCATCCTTAA AACCTGATGT GCCACCAAAG 
CCTTCCTTTG TTCCGCAAAC CACATCTGTC AGACCACTGA ACAAGTACAC GTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( NM_001290997.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277926.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 3, mRNA.

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CloneID	OMu20440
Clone ID Related Accession (Same CDS sequence)	XM_011239430.1 , NM_199238.3 , XM_030249372.1 , XM_030249373.1 , NM_001290997.1
Accession Version	NM_001290997.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-10
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform Sema6D-2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL929570.7, BY242475.1, AL833808.4, BC060680.1 and AL935323.6. Transcript Variant: This variant (3) differs in the 5' UTR, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (Sema6D-2) is shorter than isoform Sema6D-1. Both variants 2 and 3 encode isoform Sema6D-2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK122515.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN01164132, SAMN01164136 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAATCC 
AATCAGATGG TCCACATGAA TGTCCTCATC ACCTGCGTGT TTGCCGCTTT TGTCTTGGGC 
GCGTTCATCG CAGGAGTGGC CGTGTACTGC TACCGTGACA TGTTCGTTCG GAAGAACAGA 
AAGATCCATA AAGACGCAGA ATCCGCCCAG TCGTGCACAG ACTCCAGCGG AAGCTTCGCC 
AAGCTGAACG GCCTCTTTGA CAGCCCCGTC AAGGAATACC AGCAGAACAT TGATTCTCCC 
AAACTCTACA GCAACCTGCT GACCAGTCGG AAGGAACTGC CACCAAACAC GGATACAAAG 
TCCATGGCCG TGGACCACAG AGGCCAGCCT CCCGAGCTGG CTGCTCTCCC CACGCCGGAA 
TCCACACCTG TCCTCCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC TGAGAAGGCC 
CACAGCCACG GTGCTTCAAG GAAAGAACAC CCCCAGTTTT TTCCTTCTAG TCCTCCACCC 
CATTCCCCAT TGAGTCACGG GCATATCCCC AGTGCCATCG TTCTTCCAAA CGCCACTCAC 
GACTACAATA CATCCTTCTC CAACTCGAAT GCCCACAAAG CCGAAAAGAA GCTTCAGAGC 
ATGGATCACC CTCTTACGAA GTCATCCAGT AAGCGGGAGC ACCGGCGGTC TGTGGATTCC 
AGGAATACTC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC 
ATCCTGGGAG AGATCCATAT GGCTCATCAA ACCCTCATGC TGGACCCGGT GGGACCAATG 
GCTGAGGTCC CACCCAAGGT CCCTAACCGG GAGGCATCTC TATACTCCCC TCCCTCCACA 
CTCCCCAGAA ATAGTCCAAC CAAGAGAGTA GATGTCCCCA CCACTCCTGG GGTGCCAATG 
ACTTCTCTGG AAAGACAAAG GGGTTATCAC AAAAATTCCT CCCAGAGGCA CTCTATATCT 
GCCGTGCCTA AAAACTTAAA CTCACCAAAT GGTGTTTTGT TATCTAGACA GCCGAGTATG 
AACCGTGGAG GCTATATGCC CACCCCAACA GGGGCGAAGG TGGACTATAT TCAGGGGACA 
CCGGTGAGTG TTCATCTGCA GCCCTCCCTC TCCAGACAGA GCAGCTATAC CAGTAATGGC 
ACCCTCCCCA GGACGGGACT AAAGAGGACA CCATCCTTAA AACCTGATGT GCCACCAAAG 
CCTTCCTTTG TTCCGCAAAC CACATCTGTC AGACCACTGA ACAAGTACAC GTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277926.1
CDS	702..3698
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	NM_001290997.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290997.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAATCC 
AATCAGATGG TCCACATGAA TGTCCTCATC ACCTGCGTGT TTGCCGCTTT TGTCTTGGGC 
GCGTTCATCG CAGGAGTGGC CGTGTACTGC TACCGTGACA TGTTCGTTCG GAAGAACAGA 
AAGATCCATA AAGACGCAGA ATCCGCCCAG TCGTGCACAG ACTCCAGCGG AAGCTTCGCC 
AAGCTGAACG GCCTCTTTGA CAGCCCCGTC AAGGAATACC AGCAGAACAT TGATTCTCCC 
AAACTCTACA GCAACCTGCT GACCAGTCGG AAGGAACTGC CACCAAACAC GGATACAAAG 
TCCATGGCCG TGGACCACAG AGGCCAGCCT CCCGAGCTGG CTGCTCTCCC CACGCCGGAA 
TCCACACCTG TCCTCCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC TGAGAAGGCC 
CACAGCCACG GTGCTTCAAG GAAAGAACAC CCCCAGTTTT TTCCTTCTAG TCCTCCACCC 
CATTCCCCAT TGAGTCACGG GCATATCCCC AGTGCCATCG TTCTTCCAAA CGCCACTCAC 
GACTACAATA CATCCTTCTC CAACTCGAAT GCCCACAAAG CCGAAAAGAA GCTTCAGAGC 
ATGGATCACC CTCTTACGAA GTCATCCAGT AAGCGGGAGC ACCGGCGGTC TGTGGATTCC 
AGGAATACTC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC 
ATCCTGGGAG AGATCCATAT GGCTCATCAA ACCCTCATGC TGGACCCGGT GGGACCAATG 
GCTGAGGTCC CACCCAAGGT CCCTAACCGG GAGGCATCTC TATACTCCCC TCCCTCCACA 
CTCCCCAGAA ATAGTCCAAC CAAGAGAGTA GATGTCCCCA CCACTCCTGG GGTGCCAATG 
ACTTCTCTGG AAAGACAAAG GGGTTATCAC AAAAATTCCT CCCAGAGGCA CTCTATATCT 
GCCGTGCCTA AAAACTTAAA CTCACCAAAT GGTGTTTTGT TATCTAGACA GCCGAGTATG 
AACCGTGGAG GCTATATGCC CACCCCAACA GGGGCGAAGG TGGACTATAT TCAGGGGACA 
CCGGTGAGTG TTCATCTGCA GCCCTCCCTC TCCAGACAGA GCAGCTATAC CAGTAATGGC 
ACCCTCCCCA GGACGGGACT AAAGAGGACA CCATCCTTAA AACCTGATGT GCCACCAAAG 
CCTTCCTTTG TTCCGCAAAC CACATCTGTC AGACCACTGA ACAAGTACAC GTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_011239430.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237732.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMu20440
Clone ID Related Accession (Same CDS sequence)	XM_011239430.1 , NM_199238.3 , XM_030249372.1 , XM_030249373.1 , NM_001290997.1
Accession Version	XM_011239430.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	semaphorin-6D isoform X4
Comment

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAATCC 
AATCAGATGG TCCACATGAA TGTCCTCATC ACCTGCGTGT TTGCCGCTTT TGTCTTGGGC 
GCGTTCATCG CAGGAGTGGC CGTGTACTGC TACCGTGACA TGTTCGTTCG GAAGAACAGA 
AAGATCCATA AAGACGCAGA ATCCGCCCAG TCGTGCACAG ACTCCAGCGG AAGCTTCGCC 
AAGCTGAACG GCCTCTTTGA CAGCCCCGTC AAGGAATACC AGCAGAACAT TGATTCTCCC 
AAACTCTACA GCAACCTGCT GACCAGTCGG AAGGAACTGC CACCAAACAC GGATACAAAG 
TCCATGGCCG TGGACCACAG AGGCCAGCCT CCCGAGCTGG CTGCTCTCCC CACGCCGGAA 
TCCACACCTG TCCTCCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC TGAGAAGGCC 
CACAGCCACG GTGCTTCAAG GAAAGAACAC CCCCAGTTTT TTCCTTCTAG TCCTCCACCC 
CATTCCCCAT TGAGTCACGG GCATATCCCC AGTGCCATCG TTCTTCCAAA CGCCACTCAC 
GACTACAATA CATCCTTCTC CAACTCGAAT GCCCACAAAG CCGAAAAGAA GCTTCAGAGC 
ATGGATCACC CTCTTACGAA GTCATCCAGT AAGCGGGAGC ACCGGCGGTC TGTGGATTCC 
AGGAATACTC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC 
ATCCTGGGAG AGATCCATAT GGCTCATCAA ACCCTCATGC TGGACCCGGT GGGACCAATG 
GCTGAGGTCC CACCCAAGGT CCCTAACCGG GAGGCATCTC TATACTCCCC TCCCTCCACA 
CTCCCCAGAA ATAGTCCAAC CAAGAGAGTA GATGTCCCCA CCACTCCTGG GGTGCCAATG 
ACTTCTCTGG AAAGACAAAG GGGTTATCAC AAAAATTCCT CCCAGAGGCA CTCTATATCT 
GCCGTGCCTA AAAACTTAAA CTCACCAAAT GGTGTTTTGT TATCTAGACA GCCGAGTATG 
AACCGTGGAG GCTATATGCC CACCCCAACA GGGGCGAAGG TGGACTATAT TCAGGGGACA 
CCGGTGAGTG TTCATCTGCA GCCCTCCCTC TCCAGACAGA GCAGCTATAC CAGTAATGGC 
ACCCTCCCCA GGACGGGACT AAAGAGGACA CCATCCTTAA AACCTGATGT GCCACCAAAG 
CCTTCCTTTG TTCCGCAAAC CACATCTGTC AGACCACTGA ACAAGTACAC GTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011237732.1

Translation

MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_011239430.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239430.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAATCC 
AATCAGATGG TCCACATGAA TGTCCTCATC ACCTGCGTGT TTGCCGCTTT TGTCTTGGGC 
GCGTTCATCG CAGGAGTGGC CGTGTACTGC TACCGTGACA TGTTCGTTCG GAAGAACAGA 
AAGATCCATA AAGACGCAGA ATCCGCCCAG TCGTGCACAG ACTCCAGCGG AAGCTTCGCC 
AAGCTGAACG GCCTCTTTGA CAGCCCCGTC AAGGAATACC AGCAGAACAT TGATTCTCCC 
AAACTCTACA GCAACCTGCT GACCAGTCGG AAGGAACTGC CACCAAACAC GGATACAAAG 
TCCATGGCCG TGGACCACAG AGGCCAGCCT CCCGAGCTGG CTGCTCTCCC CACGCCGGAA 
TCCACACCTG TCCTCCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC TGAGAAGGCC 
CACAGCCACG GTGCTTCAAG GAAAGAACAC CCCCAGTTTT TTCCTTCTAG TCCTCCACCC 
CATTCCCCAT TGAGTCACGG GCATATCCCC AGTGCCATCG TTCTTCCAAA CGCCACTCAC 
GACTACAATA CATCCTTCTC CAACTCGAAT GCCCACAAAG CCGAAAAGAA GCTTCAGAGC 
ATGGATCACC CTCTTACGAA GTCATCCAGT AAGCGGGAGC ACCGGCGGTC TGTGGATTCC 
AGGAATACTC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC 
ATCCTGGGAG AGATCCATAT GGCTCATCAA ACCCTCATGC TGGACCCGGT GGGACCAATG 
GCTGAGGTCC CACCCAAGGT CCCTAACCGG GAGGCATCTC TATACTCCCC TCCCTCCACA 
CTCCCCAGAA ATAGTCCAAC CAAGAGAGTA GATGTCCCCA CCACTCCTGG GGTGCCAATG 
ACTTCTCTGG AAAGACAAAG GGGTTATCAC AAAAATTCCT CCCAGAGGCA CTCTATATCT 
GCCGTGCCTA AAAACTTAAA CTCACCAAAT GGTGTTTTGT TATCTAGACA GCCGAGTATG 
AACCGTGGAG GCTATATGCC CACCCCAACA GGGGCGAAGG TGGACTATAT TCAGGGGACA 
CCGGTGAGTG TTCATCTGCA GCCCTCCCTC TCCAGACAGA GCAGCTATAC CAGTAATGGC 
ACCCTCCCCA GGACGGGACT AAAGAGGACA CCATCCTTAA AACCTGATGT GCCACCAAAG 
CCTTCCTTTG TTCCGCAAAC CACATCTGTC AGACCACTGA ACAAGTACAC GTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_030249372.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105232.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X28, mRNA.

Price & Availability↑

CloneID	OMu20440
Clone ID Related Accession (Same CDS sequence)	XM_011239430.1 , NM_199238.3 , XM_030249372.1 , XM_030249373.1 , NM_001290997.1
Accession Version	XM_030249372.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAATCC 
AATCAGATGG TCCACATGAA TGTCCTCATC ACCTGCGTGT TTGCCGCTTT TGTCTTGGGC 
GCGTTCATCG CAGGAGTGGC CGTGTACTGC TACCGTGACA TGTTCGTTCG GAAGAACAGA 
AAGATCCATA AAGACGCAGA ATCCGCCCAG TCGTGCACAG ACTCCAGCGG AAGCTTCGCC 
AAGCTGAACG GCCTCTTTGA CAGCCCCGTC AAGGAATACC AGCAGAACAT TGATTCTCCC 
AAACTCTACA GCAACCTGCT GACCAGTCGG AAGGAACTGC CACCAAACAC GGATACAAAG 
TCCATGGCCG TGGACCACAG AGGCCAGCCT CCCGAGCTGG CTGCTCTCCC CACGCCGGAA 
TCCACACCTG TCCTCCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC TGAGAAGGCC 
CACAGCCACG GTGCTTCAAG GAAAGAACAC CCCCAGTTTT TTCCTTCTAG TCCTCCACCC 
CATTCCCCAT TGAGTCACGG GCATATCCCC AGTGCCATCG TTCTTCCAAA CGCCACTCAC 
GACTACAATA CATCCTTCTC CAACTCGAAT GCCCACAAAG CCGAAAAGAA GCTTCAGAGC 
ATGGATCACC CTCTTACGAA GTCATCCAGT AAGCGGGAGC ACCGGCGGTC TGTGGATTCC 
AGGAATACTC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC 
ATCCTGGGAG AGATCCATAT GGCTCATCAA ACCCTCATGC TGGACCCGGT GGGACCAATG 
GCTGAGGTCC CACCCAAGGT CCCTAACCGG GAGGCATCTC TATACTCCCC TCCCTCCACA 
CTCCCCAGAA ATAGTCCAAC CAAGAGAGTA GATGTCCCCA CCACTCCTGG GGTGCCAATG 
ACTTCTCTGG AAAGACAAAG GGGTTATCAC AAAAATTCCT CCCAGAGGCA CTCTATATCT 
GCCGTGCCTA AAAACTTAAA CTCACCAAAT GGTGTTTTGT TATCTAGACA GCCGAGTATG 
AACCGTGGAG GCTATATGCC CACCCCAACA GGGGCGAAGG TGGACTATAT TCAGGGGACA 
CCGGTGAGTG TTCATCTGCA GCCCTCCCTC TCCAGACAGA GCAGCTATAC CAGTAATGGC 
ACCCTCCCCA GGACGGGACT AAAGAGGACA CCATCCTTAA AACCTGATGT GCCACCAAAG 
CCTTCCTTTG TTCCGCAAAC CACATCTGTC AGACCACTGA ACAAGTACAC GTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105232.1
CDS	605..3601
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_030249372.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249372.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAATCC 
AATCAGATGG TCCACATGAA TGTCCTCATC ACCTGCGTGT TTGCCGCTTT TGTCTTGGGC 
GCGTTCATCG CAGGAGTGGC CGTGTACTGC TACCGTGACA TGTTCGTTCG GAAGAACAGA 
AAGATCCATA AAGACGCAGA ATCCGCCCAG TCGTGCACAG ACTCCAGCGG AAGCTTCGCC 
AAGCTGAACG GCCTCTTTGA CAGCCCCGTC AAGGAATACC AGCAGAACAT TGATTCTCCC 
AAACTCTACA GCAACCTGCT GACCAGTCGG AAGGAACTGC CACCAAACAC GGATACAAAG 
TCCATGGCCG TGGACCACAG AGGCCAGCCT CCCGAGCTGG CTGCTCTCCC CACGCCGGAA 
TCCACACCTG TCCTCCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC TGAGAAGGCC 
CACAGCCACG GTGCTTCAAG GAAAGAACAC CCCCAGTTTT TTCCTTCTAG TCCTCCACCC 
CATTCCCCAT TGAGTCACGG GCATATCCCC AGTGCCATCG TTCTTCCAAA CGCCACTCAC 
GACTACAATA CATCCTTCTC CAACTCGAAT GCCCACAAAG CCGAAAAGAA GCTTCAGAGC 
ATGGATCACC CTCTTACGAA GTCATCCAGT AAGCGGGAGC ACCGGCGGTC TGTGGATTCC 
AGGAATACTC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC 
ATCCTGGGAG AGATCCATAT GGCTCATCAA ACCCTCATGC TGGACCCGGT GGGACCAATG 
GCTGAGGTCC CACCCAAGGT CCCTAACCGG GAGGCATCTC TATACTCCCC TCCCTCCACA 
CTCCCCAGAA ATAGTCCAAC CAAGAGAGTA GATGTCCCCA CCACTCCTGG GGTGCCAATG 
ACTTCTCTGG AAAGACAAAG GGGTTATCAC AAAAATTCCT CCCAGAGGCA CTCTATATCT 
GCCGTGCCTA AAAACTTAAA CTCACCAAAT GGTGTTTTGT TATCTAGACA GCCGAGTATG 
AACCGTGGAG GCTATATGCC CACCCCAACA GGGGCGAAGG TGGACTATAT TCAGGGGACA 
CCGGTGAGTG TTCATCTGCA GCCCTCCCTC TCCAGACAGA GCAGCTATAC CAGTAATGGC 
ACCCTCCCCA GGACGGGACT AAAGAGGACA CCATCCTTAA AACCTGATGT GCCACCAAAG 
CCTTCCTTTG TTCCGCAAAC CACATCTGTC AGACCACTGA ACAAGTACAC GTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_030249373.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105233.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X29, mRNA.

Price & Availability↑

CloneID	OMu20440
Clone ID Related Accession (Same CDS sequence)	XM_011239430.1 , NM_199238.3 , XM_030249372.1 , XM_030249373.1 , NM_001290997.1
Accession Version	XM_030249373.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAATCC 
AATCAGATGG TCCACATGAA TGTCCTCATC ACCTGCGTGT TTGCCGCTTT TGTCTTGGGC 
GCGTTCATCG CAGGAGTGGC CGTGTACTGC TACCGTGACA TGTTCGTTCG GAAGAACAGA 
AAGATCCATA AAGACGCAGA ATCCGCCCAG TCGTGCACAG ACTCCAGCGG AAGCTTCGCC 
AAGCTGAACG GCCTCTTTGA CAGCCCCGTC AAGGAATACC AGCAGAACAT TGATTCTCCC 
AAACTCTACA GCAACCTGCT GACCAGTCGG AAGGAACTGC CACCAAACAC GGATACAAAG 
TCCATGGCCG TGGACCACAG AGGCCAGCCT CCCGAGCTGG CTGCTCTCCC CACGCCGGAA 
TCCACACCTG TCCTCCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC TGAGAAGGCC 
CACAGCCACG GTGCTTCAAG GAAAGAACAC CCCCAGTTTT TTCCTTCTAG TCCTCCACCC 
CATTCCCCAT TGAGTCACGG GCATATCCCC AGTGCCATCG TTCTTCCAAA CGCCACTCAC 
GACTACAATA CATCCTTCTC CAACTCGAAT GCCCACAAAG CCGAAAAGAA GCTTCAGAGC 
ATGGATCACC CTCTTACGAA GTCATCCAGT AAGCGGGAGC ACCGGCGGTC TGTGGATTCC 
AGGAATACTC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC 
ATCCTGGGAG AGATCCATAT GGCTCATCAA ACCCTCATGC TGGACCCGGT GGGACCAATG 
GCTGAGGTCC CACCCAAGGT CCCTAACCGG GAGGCATCTC TATACTCCCC TCCCTCCACA 
CTCCCCAGAA ATAGTCCAAC CAAGAGAGTA GATGTCCCCA CCACTCCTGG GGTGCCAATG 
ACTTCTCTGG AAAGACAAAG GGGTTATCAC AAAAATTCCT CCCAGAGGCA CTCTATATCT 
GCCGTGCCTA AAAACTTAAA CTCACCAAAT GGTGTTTTGT TATCTAGACA GCCGAGTATG 
AACCGTGGAG GCTATATGCC CACCCCAACA GGGGCGAAGG TGGACTATAT TCAGGGGACA 
CCGGTGAGTG TTCATCTGCA GCCCTCCCTC TCCAGACAGA GCAGCTATAC CAGTAATGGC 
ACCCTCCCCA GGACGGGACT AAAGAGGACA CCATCCTTAA AACCTGATGT GCCACCAAAG 
CCTTCCTTTG TTCCGCAAAC CACATCTGTC AGACCACTGA ACAAGTACAC GTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105233.1
CDS	8748..11744
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_030249373.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249373.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAATCC 
AATCAGATGG TCCACATGAA TGTCCTCATC ACCTGCGTGT TTGCCGCTTT TGTCTTGGGC 
GCGTTCATCG CAGGAGTGGC CGTGTACTGC TACCGTGACA TGTTCGTTCG GAAGAACAGA 
AAGATCCATA AAGACGCAGA ATCCGCCCAG TCGTGCACAG ACTCCAGCGG AAGCTTCGCC 
AAGCTGAACG GCCTCTTTGA CAGCCCCGTC AAGGAATACC AGCAGAACAT TGATTCTCCC 
AAACTCTACA GCAACCTGCT GACCAGTCGG AAGGAACTGC CACCAAACAC GGATACAAAG 
TCCATGGCCG TGGACCACAG AGGCCAGCCT CCCGAGCTGG CTGCTCTCCC CACGCCGGAA 
TCCACACCTG TCCTCCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC TGAGAAGGCC 
CACAGCCACG GTGCTTCAAG GAAAGAACAC CCCCAGTTTT TTCCTTCTAG TCCTCCACCC 
CATTCCCCAT TGAGTCACGG GCATATCCCC AGTGCCATCG TTCTTCCAAA CGCCACTCAC 
GACTACAATA CATCCTTCTC CAACTCGAAT GCCCACAAAG CCGAAAAGAA GCTTCAGAGC 
ATGGATCACC CTCTTACGAA GTCATCCAGT AAGCGGGAGC ACCGGCGGTC TGTGGATTCC 
AGGAATACTC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC 
ATCCTGGGAG AGATCCATAT GGCTCATCAA ACCCTCATGC TGGACCCGGT GGGACCAATG 
GCTGAGGTCC CACCCAAGGT CCCTAACCGG GAGGCATCTC TATACTCCCC TCCCTCCACA 
CTCCCCAGAA ATAGTCCAAC CAAGAGAGTA GATGTCCCCA CCACTCCTGG GGTGCCAATG 
ACTTCTCTGG AAAGACAAAG GGGTTATCAC AAAAATTCCT CCCAGAGGCA CTCTATATCT 
GCCGTGCCTA AAAACTTAAA CTCACCAAAT GGTGTTTTGT TATCTAGACA GCCGAGTATG 
AACCGTGGAG GCTATATGCC CACCCCAACA GGGGCGAAGG TGGACTATAT TCAGGGGACA 
CCGGTGAGTG TTCATCTGCA GCCCTCCCTC TCCAGACAGA GCAGCTATAC CAGTAATGGC 
ACCCTCCCCA GGACGGGACT AAAGAGGACA CCATCCTTAA AACCTGATGT GCCACCAAAG 
CCTTCCTTTG TTCCGCAAAC CACATCTGTC AGACCACTGA ACAAGTACAC GTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_017317019.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172508.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X23, mRNA.

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CloneID	OMu20434
Clone ID Related Accession (Same CDS sequence)	XM_017317019.2 , XM_017317019.1 , NM_199239.3
Accession Version	XM_017317019.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317019.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC 
CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG 
TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC 
GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC 
CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC 
AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC 
CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG 
ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA 
GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT 
ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC 
TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA 
TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG 
AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT 
CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT 
AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG 
AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT 
TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA 
CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC 
CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC 
TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG 
AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA 
TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172508.1
CDS	830..3922
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_017317019.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317019.2

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC 
CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG 
TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC 
GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC 
CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC 
AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC 
CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG 
ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA 
GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT 
ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC 
TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA 
TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG 
AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT 
CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT 
AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG 
AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT 
TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA 
CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC 
CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC 
TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG 
AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA 
TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_030249369.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105229.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X26, mRNA.

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CloneID	OMu20449
Clone ID Related Accession (Same CDS sequence)	XM_030249369.1 , XM_030249371.1 , NM_172537.4
Accession Version	XM_030249369.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGGTG TACGGTGGGA AGTCCAGTCT GGAGAATCCA ATCAGATGGT CCACATGAAT 
GTCCTCATCA CCTGCGTGTT TGCCGCTTTT GTCTTGGGCG CGTTCATCGC AGGAGTGGCC 
GTGTACTGCT ACCGTGACAT GTTCGTTCGG AAGAACAGAA AGATCCATAA AGACGCAGAA 
TCCGCCCAGT CGTGCACAGA CTCCAGCGGA AGCTTCGCCA AGCTGAACGG CCTCTTTGAC 
AGCCCCGTCA AGGAATACCA GCAGAACATT GATTCTCCCA AACTCTACAG CAACCTGCTG 
ACCAGTCGGA AGGAACTGCC ACCAAACACG GATACAAAGT CCATGGCCGT GGACCACAGA 
GGCCAGCCTC CCGAGCTGGC TGCTCTCCCC ACGCCGGAAT CCACACCTGT CCTCCACCAG 
AAGACCCTGC AGGCCATGAA GAGCCACTCT GAGAAGGCCC ACAGCCACGG TGCTTCAAGG 
AAAGAACACC CCCAGTTTTT TCCTTCTAGT CCTCCACCCC ATTCCCCATT GAGTCACGGG 
CATATCCCCA GTGCCATCGT TCTTCCAAAC GCCACTCACG ACTACAATAC ATCCTTCTCC 
AACTCGAATG CCCACAAAGC CGAAAAGAAG CTTCAGAGCA TGGATCACCC TCTTACGAAG 
TCATCCAGTA AGCGGGAGCA CCGGCGGTCT GTGGATTCCA GGAATACTCT CAATGATCTC 
CTGAAGCATC TAAATGACCC AAACAGTAAC CCCAAAGCCA TCCTGGGAGA GATCCATATG 
GCTCATCAAA CCCTCATGCT GGACCCGGTG GGACCAATGG CTGAGGTCCC ACCCAAGGTC 
CCTAACCGGG AGGCATCTCT ATACTCCCCT CCCTCCACAC TCCCCAGAAA TAGTCCAACC 
AAGAGAGTAG ATGTCCCCAC CACTCCTGGG GTGCCAATGA CTTCTCTGGA AAGACAAAGG 
GGTTATCACA AAAATTCCTC CCAGAGGCAC TCTATATCTG CCGTGCCTAA AAACTTAAAC 
TCACCAAATG GTGTTTTGTT ATCTAGACAG CCGAGTATGA ACCGTGGAGG CTATATGCCC 
ACCCCAACAG GGGCGAAGGT GGACTATATT CAGGGGACAC CGGTGAGTGT TCATCTGCAG 
CCCTCCCTCT CCAGACAGAG CAGCTATACC AGTAATGGCA CCCTCCCCAG GACGGGACTA 
AAGAGGACAC CATCCTTAAA ACCTGATGTG CCACCAAAGC CTTCCTTTGT TCCGCAAACC 
ACATCTGTCA GACCACTGAA CAAGTACACG TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105229.1
CDS	605..3640
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_030249369.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249369.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGGTG TACGGTGGGA AGTCCAGTCT GGAGAATCCA ATCAGATGGT CCACATGAAT 
GTCCTCATCA CCTGCGTGTT TGCCGCTTTT GTCTTGGGCG CGTTCATCGC AGGAGTGGCC 
GTGTACTGCT ACCGTGACAT GTTCGTTCGG AAGAACAGAA AGATCCATAA AGACGCAGAA 
TCCGCCCAGT CGTGCACAGA CTCCAGCGGA AGCTTCGCCA AGCTGAACGG CCTCTTTGAC 
AGCCCCGTCA AGGAATACCA GCAGAACATT GATTCTCCCA AACTCTACAG CAACCTGCTG 
ACCAGTCGGA AGGAACTGCC ACCAAACACG GATACAAAGT CCATGGCCGT GGACCACAGA 
GGCCAGCCTC CCGAGCTGGC TGCTCTCCCC ACGCCGGAAT CCACACCTGT CCTCCACCAG 
AAGACCCTGC AGGCCATGAA GAGCCACTCT GAGAAGGCCC ACAGCCACGG TGCTTCAAGG 
AAAGAACACC CCCAGTTTTT TCCTTCTAGT CCTCCACCCC ATTCCCCATT GAGTCACGGG 
CATATCCCCA GTGCCATCGT TCTTCCAAAC GCCACTCACG ACTACAATAC ATCCTTCTCC 
AACTCGAATG CCCACAAAGC CGAAAAGAAG CTTCAGAGCA TGGATCACCC TCTTACGAAG 
TCATCCAGTA AGCGGGAGCA CCGGCGGTCT GTGGATTCCA GGAATACTCT CAATGATCTC 
CTGAAGCATC TAAATGACCC AAACAGTAAC CCCAAAGCCA TCCTGGGAGA GATCCATATG 
GCTCATCAAA CCCTCATGCT GGACCCGGTG GGACCAATGG CTGAGGTCCC ACCCAAGGTC 
CCTAACCGGG AGGCATCTCT ATACTCCCCT CCCTCCACAC TCCCCAGAAA TAGTCCAACC 
AAGAGAGTAG ATGTCCCCAC CACTCCTGGG GTGCCAATGA CTTCTCTGGA AAGACAAAGG 
GGTTATCACA AAAATTCCTC CCAGAGGCAC TCTATATCTG CCGTGCCTAA AAACTTAAAC 
TCACCAAATG GTGTTTTGTT ATCTAGACAG CCGAGTATGA ACCGTGGAGG CTATATGCCC 
ACCCCAACAG GGGCGAAGGT GGACTATATT CAGGGGACAC CGGTGAGTGT TCATCTGCAG 
CCCTCCCTCT CCAGACAGAG CAGCTATACC AGTAATGGCA CCCTCCCCAG GACGGGACTA 
AAGAGGACAC CATCCTTAAA ACCTGATGTG CCACCAAAGC CTTCCTTTGT TCCGCAAACC 
ACATCTGTCA GACCACTGAA CAAGTACACG TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_030249371.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105231.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X27, mRNA.

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CloneID	OMu20449
Clone ID Related Accession (Same CDS sequence)	XM_030249369.1 , XM_030249371.1 , NM_172537.4
Accession Version	XM_030249371.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGGTG TACGGTGGGA AGTCCAGTCT GGAGAATCCA ATCAGATGGT CCACATGAAT 
GTCCTCATCA CCTGCGTGTT TGCCGCTTTT GTCTTGGGCG CGTTCATCGC AGGAGTGGCC 
GTGTACTGCT ACCGTGACAT GTTCGTTCGG AAGAACAGAA AGATCCATAA AGACGCAGAA 
TCCGCCCAGT CGTGCACAGA CTCCAGCGGA AGCTTCGCCA AGCTGAACGG CCTCTTTGAC 
AGCCCCGTCA AGGAATACCA GCAGAACATT GATTCTCCCA AACTCTACAG CAACCTGCTG 
ACCAGTCGGA AGGAACTGCC ACCAAACACG GATACAAAGT CCATGGCCGT GGACCACAGA 
GGCCAGCCTC CCGAGCTGGC TGCTCTCCCC ACGCCGGAAT CCACACCTGT CCTCCACCAG 
AAGACCCTGC AGGCCATGAA GAGCCACTCT GAGAAGGCCC ACAGCCACGG TGCTTCAAGG 
AAAGAACACC CCCAGTTTTT TCCTTCTAGT CCTCCACCCC ATTCCCCATT GAGTCACGGG 
CATATCCCCA GTGCCATCGT TCTTCCAAAC GCCACTCACG ACTACAATAC ATCCTTCTCC 
AACTCGAATG CCCACAAAGC CGAAAAGAAG CTTCAGAGCA TGGATCACCC TCTTACGAAG 
TCATCCAGTA AGCGGGAGCA CCGGCGGTCT GTGGATTCCA GGAATACTCT CAATGATCTC 
CTGAAGCATC TAAATGACCC AAACAGTAAC CCCAAAGCCA TCCTGGGAGA GATCCATATG 
GCTCATCAAA CCCTCATGCT GGACCCGGTG GGACCAATGG CTGAGGTCCC ACCCAAGGTC 
CCTAACCGGG AGGCATCTCT ATACTCCCCT CCCTCCACAC TCCCCAGAAA TAGTCCAACC 
AAGAGAGTAG ATGTCCCCAC CACTCCTGGG GTGCCAATGA CTTCTCTGGA AAGACAAAGG 
GGTTATCACA AAAATTCCTC CCAGAGGCAC TCTATATCTG CCGTGCCTAA AAACTTAAAC 
TCACCAAATG GTGTTTTGTT ATCTAGACAG CCGAGTATGA ACCGTGGAGG CTATATGCCC 
ACCCCAACAG GGGCGAAGGT GGACTATATT CAGGGGACAC CGGTGAGTGT TCATCTGCAG 
CCCTCCCTCT CCAGACAGAG CAGCTATACC AGTAATGGCA CCCTCCCCAG GACGGGACTA 
AAGAGGACAC CATCCTTAAA ACCTGATGTG CCACCAAAGC CTTCCTTTGT TCCGCAAACC 
ACATCTGTCA GACCACTGAA CAAGTACACG TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105231.1
CDS	8748..11783
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_030249371.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249371.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGGTG TACGGTGGGA AGTCCAGTCT GGAGAATCCA ATCAGATGGT CCACATGAAT 
GTCCTCATCA CCTGCGTGTT TGCCGCTTTT GTCTTGGGCG CGTTCATCGC AGGAGTGGCC 
GTGTACTGCT ACCGTGACAT GTTCGTTCGG AAGAACAGAA AGATCCATAA AGACGCAGAA 
TCCGCCCAGT CGTGCACAGA CTCCAGCGGA AGCTTCGCCA AGCTGAACGG CCTCTTTGAC 
AGCCCCGTCA AGGAATACCA GCAGAACATT GATTCTCCCA AACTCTACAG CAACCTGCTG 
ACCAGTCGGA AGGAACTGCC ACCAAACACG GATACAAAGT CCATGGCCGT GGACCACAGA 
GGCCAGCCTC CCGAGCTGGC TGCTCTCCCC ACGCCGGAAT CCACACCTGT CCTCCACCAG 
AAGACCCTGC AGGCCATGAA GAGCCACTCT GAGAAGGCCC ACAGCCACGG TGCTTCAAGG 
AAAGAACACC CCCAGTTTTT TCCTTCTAGT CCTCCACCCC ATTCCCCATT GAGTCACGGG 
CATATCCCCA GTGCCATCGT TCTTCCAAAC GCCACTCACG ACTACAATAC ATCCTTCTCC 
AACTCGAATG CCCACAAAGC CGAAAAGAAG CTTCAGAGCA TGGATCACCC TCTTACGAAG 
TCATCCAGTA AGCGGGAGCA CCGGCGGTCT GTGGATTCCA GGAATACTCT CAATGATCTC 
CTGAAGCATC TAAATGACCC AAACAGTAAC CCCAAAGCCA TCCTGGGAGA GATCCATATG 
GCTCATCAAA CCCTCATGCT GGACCCGGTG GGACCAATGG CTGAGGTCCC ACCCAAGGTC 
CCTAACCGGG AGGCATCTCT ATACTCCCCT CCCTCCACAC TCCCCAGAAA TAGTCCAACC 
AAGAGAGTAG ATGTCCCCAC CACTCCTGGG GTGCCAATGA CTTCTCTGGA AAGACAAAGG 
GGTTATCACA AAAATTCCTC CCAGAGGCAC TCTATATCTG CCGTGCCTAA AAACTTAAAC 
TCACCAAATG GTGTTTTGTT ATCTAGACAG CCGAGTATGA ACCGTGGAGG CTATATGCCC 
ACCCCAACAG GGGCGAAGGT GGACTATATT CAGGGGACAC CGGTGAGTGT TCATCTGCAG 
CCCTCCCTCT CCAGACAGAG CAGCTATACC AGTAATGGCA CCCTCCCCAG GACGGGACTA 
AAGAGGACAC CATCCTTAAA ACCTGATGTG CCACCAAAGC CTTCCTTTGT TCCGCAAACC 
ACATCTGTCA GACCACTGAA CAAGTACACG TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_030249343.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105203.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu20490
Clone ID Related Accession (Same CDS sequence)	XM_030249343.1 , XM_030249345.1 , XM_017317001.1 , XM_017317001.2 , NM_199241.3
Accession Version	XM_030249343.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG 
GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC 
TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA 
GGTATCCCAA AGGGTGTACG GTGGGAAGTC CAGTCTGGAG AATCCAATCA GATGGTCCAC 
ATGAATGTCC TCATCACCTG CGTGTTTGCC GCTTTTGTCT TGGGCGCGTT CATCGCAGGA 
GTGGCCGTGT ACTGCTACCG TGACATGTTC GTTCGGAAGA ACAGAAAGAT CCATAAAGAC 
GCAGAATCCG CCCAGTCGTG CACAGACTCC AGCGGAAGCT TCGCCAAGCT GAACGGCCTC 
TTTGACAGCC CCGTCAAGGA ATACCAGCAG AACATTGATT CTCCCAAACT CTACAGCAAC 
CTGCTGACCA GTCGGAAGGA ACTGCCACCA AACACGGATA CAAAGTCCAT GGCCGTGGAC 
CACAGAGGCC AGCCTCCCGA GCTGGCTGCT CTCCCCACGC CGGAATCCAC ACCTGTCCTC 
CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCTGAGA AGGCCCACAG CCACGGTGCT 
TCAAGGAAAG AACACCCCCA GTTTTTTCCT TCTAGTCCTC CACCCCATTC CCCATTGAGT 
CACGGGCATA TCCCCAGTGC CATCGTTCTT CCAAACGCCA CTCACGACTA CAATACATCC 
TTCTCCAACT CGAATGCCCA CAAAGCCGAA AAGAAGCTTC AGAGCATGGA TCACCCTCTT 
ACGAAGTCAT CCAGTAAGCG GGAGCACCGG CGGTCTGTGG ATTCCAGGAA TACTCTCAAT 
GATCTCCTGA AGCATCTAAA TGACCCAAAC AGTAACCCCA AAGCCATCCT GGGAGAGATC 
CATATGGCTC ATCAAACCCT CATGCTGGAC CCGGTGGGAC CAATGGCTGA GGTCCCACCC 
AAGGTCCCTA ACCGGGAGGC ATCTCTATAC TCCCCTCCCT CCACACTCCC CAGAAATAGT 
CCAACCAAGA GAGTAGATGT CCCCACCACT CCTGGGGTGC CAATGACTTC TCTGGAAAGA 
CAAAGGGGTT ATCACAAAAA TTCCTCCCAG AGGCACTCTA TATCTGCCGT GCCTAAAAAC 
TTAAACTCAC CAAATGGTGT TTTGTTATCT AGACAGCCGA GTATGAACCG TGGAGGCTAT 
ATGCCCACCC CAACAGGGGC GAAGGTGGAC TATATTCAGG GGACACCGGT GAGTGTTCAT 
CTGCAGCCCT CCCTCTCCAG ACAGAGCAGC TATACCAGTA ATGGCACCCT CCCCAGGACG 
GGACTAAAGA GGACACCATC CTTAAAACCT GATGTGCCAC CAAAGCCTTC CTTTGTTCCG 
CAAACCACAT CTGTCAGACC ACTGAACAAG TACACGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105203.1
CDS	8748..11969
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_030249343.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249343.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG 
GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC 
TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA 
GGTATCCCAA AGGGTGTACG GTGGGAAGTC CAGTCTGGAG AATCCAATCA GATGGTCCAC 
ATGAATGTCC TCATCACCTG CGTGTTTGCC GCTTTTGTCT TGGGCGCGTT CATCGCAGGA 
GTGGCCGTGT ACTGCTACCG TGACATGTTC GTTCGGAAGA ACAGAAAGAT CCATAAAGAC 
GCAGAATCCG CCCAGTCGTG CACAGACTCC AGCGGAAGCT TCGCCAAGCT GAACGGCCTC 
TTTGACAGCC CCGTCAAGGA ATACCAGCAG AACATTGATT CTCCCAAACT CTACAGCAAC 
CTGCTGACCA GTCGGAAGGA ACTGCCACCA AACACGGATA CAAAGTCCAT GGCCGTGGAC 
CACAGAGGCC AGCCTCCCGA GCTGGCTGCT CTCCCCACGC CGGAATCCAC ACCTGTCCTC 
CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCTGAGA AGGCCCACAG CCACGGTGCT 
TCAAGGAAAG AACACCCCCA GTTTTTTCCT TCTAGTCCTC CACCCCATTC CCCATTGAGT 
CACGGGCATA TCCCCAGTGC CATCGTTCTT CCAAACGCCA CTCACGACTA CAATACATCC 
TTCTCCAACT CGAATGCCCA CAAAGCCGAA AAGAAGCTTC AGAGCATGGA TCACCCTCTT 
ACGAAGTCAT CCAGTAAGCG GGAGCACCGG CGGTCTGTGG ATTCCAGGAA TACTCTCAAT 
GATCTCCTGA AGCATCTAAA TGACCCAAAC AGTAACCCCA AAGCCATCCT GGGAGAGATC 
CATATGGCTC ATCAAACCCT CATGCTGGAC CCGGTGGGAC CAATGGCTGA GGTCCCACCC 
AAGGTCCCTA ACCGGGAGGC ATCTCTATAC TCCCCTCCCT CCACACTCCC CAGAAATAGT 
CCAACCAAGA GAGTAGATGT CCCCACCACT CCTGGGGTGC CAATGACTTC TCTGGAAAGA 
CAAAGGGGTT ATCACAAAAA TTCCTCCCAG AGGCACTCTA TATCTGCCGT GCCTAAAAAC 
TTAAACTCAC CAAATGGTGT TTTGTTATCT AGACAGCCGA GTATGAACCG TGGAGGCTAT 
ATGCCCACCC CAACAGGGGC GAAGGTGGAC TATATTCAGG GGACACCGGT GAGTGTTCAT 
CTGCAGCCCT CCCTCTCCAG ACAGAGCAGC TATACCAGTA ATGGCACCCT CCCCAGGACG 
GGACTAAAGA GGACACCATC CTTAAAACCT GATGTGCCAC CAAAGCCTTC CTTTGTTCCG 
CAAACCACAT CTGTCAGACC ACTGAACAAG TACACGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_030249345.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105205.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu20490
Clone ID Related Accession (Same CDS sequence)	XM_030249343.1 , XM_030249345.1 , XM_017317001.1 , XM_017317001.2 , NM_199241.3
Accession Version	XM_030249345.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG 
GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC 
TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA 
GGTATCCCAA AGGGTGTACG GTGGGAAGTC CAGTCTGGAG AATCCAATCA GATGGTCCAC 
ATGAATGTCC TCATCACCTG CGTGTTTGCC GCTTTTGTCT TGGGCGCGTT CATCGCAGGA 
GTGGCCGTGT ACTGCTACCG TGACATGTTC GTTCGGAAGA ACAGAAAGAT CCATAAAGAC 
GCAGAATCCG CCCAGTCGTG CACAGACTCC AGCGGAAGCT TCGCCAAGCT GAACGGCCTC 
TTTGACAGCC CCGTCAAGGA ATACCAGCAG AACATTGATT CTCCCAAACT CTACAGCAAC 
CTGCTGACCA GTCGGAAGGA ACTGCCACCA AACACGGATA CAAAGTCCAT GGCCGTGGAC 
CACAGAGGCC AGCCTCCCGA GCTGGCTGCT CTCCCCACGC CGGAATCCAC ACCTGTCCTC 
CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCTGAGA AGGCCCACAG CCACGGTGCT 
TCAAGGAAAG AACACCCCCA GTTTTTTCCT TCTAGTCCTC CACCCCATTC CCCATTGAGT 
CACGGGCATA TCCCCAGTGC CATCGTTCTT CCAAACGCCA CTCACGACTA CAATACATCC 
TTCTCCAACT CGAATGCCCA CAAAGCCGAA AAGAAGCTTC AGAGCATGGA TCACCCTCTT 
ACGAAGTCAT CCAGTAAGCG GGAGCACCGG CGGTCTGTGG ATTCCAGGAA TACTCTCAAT 
GATCTCCTGA AGCATCTAAA TGACCCAAAC AGTAACCCCA AAGCCATCCT GGGAGAGATC 
CATATGGCTC ATCAAACCCT CATGCTGGAC CCGGTGGGAC CAATGGCTGA GGTCCCACCC 
AAGGTCCCTA ACCGGGAGGC ATCTCTATAC TCCCCTCCCT CCACACTCCC CAGAAATAGT 
CCAACCAAGA GAGTAGATGT CCCCACCACT CCTGGGGTGC CAATGACTTC TCTGGAAAGA 
CAAAGGGGTT ATCACAAAAA TTCCTCCCAG AGGCACTCTA TATCTGCCGT GCCTAAAAAC 
TTAAACTCAC CAAATGGTGT TTTGTTATCT AGACAGCCGA GTATGAACCG TGGAGGCTAT 
ATGCCCACCC CAACAGGGGC GAAGGTGGAC TATATTCAGG GGACACCGGT GAGTGTTCAT 
CTGCAGCCCT CCCTCTCCAG ACAGAGCAGC TATACCAGTA ATGGCACCCT CCCCAGGACG 
GGACTAAAGA GGACACCATC CTTAAAACCT GATGTGCCAC CAAAGCCTTC CTTTGTTCCG 
CAAACCACAT CTGTCAGACC ACTGAACAAG TACACGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105205.1
CDS	832..4053
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_030249345.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249345.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG 
GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC 
TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA 
GGTATCCCAA AGGGTGTACG GTGGGAAGTC CAGTCTGGAG AATCCAATCA GATGGTCCAC 
ATGAATGTCC TCATCACCTG CGTGTTTGCC GCTTTTGTCT TGGGCGCGTT CATCGCAGGA 
GTGGCCGTGT ACTGCTACCG TGACATGTTC GTTCGGAAGA ACAGAAAGAT CCATAAAGAC 
GCAGAATCCG CCCAGTCGTG CACAGACTCC AGCGGAAGCT TCGCCAAGCT GAACGGCCTC 
TTTGACAGCC CCGTCAAGGA ATACCAGCAG AACATTGATT CTCCCAAACT CTACAGCAAC 
CTGCTGACCA GTCGGAAGGA ACTGCCACCA AACACGGATA CAAAGTCCAT GGCCGTGGAC 
CACAGAGGCC AGCCTCCCGA GCTGGCTGCT CTCCCCACGC CGGAATCCAC ACCTGTCCTC 
CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCTGAGA AGGCCCACAG CCACGGTGCT 
TCAAGGAAAG AACACCCCCA GTTTTTTCCT TCTAGTCCTC CACCCCATTC CCCATTGAGT 
CACGGGCATA TCCCCAGTGC CATCGTTCTT CCAAACGCCA CTCACGACTA CAATACATCC 
TTCTCCAACT CGAATGCCCA CAAAGCCGAA AAGAAGCTTC AGAGCATGGA TCACCCTCTT 
ACGAAGTCAT CCAGTAAGCG GGAGCACCGG CGGTCTGTGG ATTCCAGGAA TACTCTCAAT 
GATCTCCTGA AGCATCTAAA TGACCCAAAC AGTAACCCCA AAGCCATCCT GGGAGAGATC 
CATATGGCTC ATCAAACCCT CATGCTGGAC CCGGTGGGAC CAATGGCTGA GGTCCCACCC 
AAGGTCCCTA ACCGGGAGGC ATCTCTATAC TCCCCTCCCT CCACACTCCC CAGAAATAGT 
CCAACCAAGA GAGTAGATGT CCCCACCACT CCTGGGGTGC CAATGACTTC TCTGGAAAGA 
CAAAGGGGTT ATCACAAAAA TTCCTCCCAG AGGCACTCTA TATCTGCCGT GCCTAAAAAC 
TTAAACTCAC CAAATGGTGT TTTGTTATCT AGACAGCCGA GTATGAACCG TGGAGGCTAT 
ATGCCCACCC CAACAGGGGC GAAGGTGGAC TATATTCAGG GGACACCGGT GAGTGTTCAT 
CTGCAGCCCT CCCTCTCCAG ACAGAGCAGC TATACCAGTA ATGGCACCCT CCCCAGGACG 
GGACTAAAGA GGACACCATC CTTAAAACCT GATGTGCCAC CAAAGCCTTC CTTTGTTCCG 
CAAACCACAT CTGTCAGACC ACTGAACAAG TACACGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_017317001.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172490.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X8, mRNA.

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CloneID	OMu20490
Clone ID Related Accession (Same CDS sequence)	XM_030249343.1 , XM_030249345.1 , XM_017317001.1 , XM_017317001.2 , NM_199241.3
Accession Version	XM_017317001.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317001.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG 
GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC 
TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA 
GGTATCCCAA AGGGTGTACG GTGGGAAGTC CAGTCTGGAG AATCCAATCA GATGGTCCAC 
ATGAATGTCC TCATCACCTG CGTGTTTGCC GCTTTTGTCT TGGGCGCGTT CATCGCAGGA 
GTGGCCGTGT ACTGCTACCG TGACATGTTC GTTCGGAAGA ACAGAAAGAT CCATAAAGAC 
GCAGAATCCG CCCAGTCGTG CACAGACTCC AGCGGAAGCT TCGCCAAGCT GAACGGCCTC 
TTTGACAGCC CCGTCAAGGA ATACCAGCAG AACATTGATT CTCCCAAACT CTACAGCAAC 
CTGCTGACCA GTCGGAAGGA ACTGCCACCA AACACGGATA CAAAGTCCAT GGCCGTGGAC 
CACAGAGGCC AGCCTCCCGA GCTGGCTGCT CTCCCCACGC CGGAATCCAC ACCTGTCCTC 
CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCTGAGA AGGCCCACAG CCACGGTGCT 
TCAAGGAAAG AACACCCCCA GTTTTTTCCT TCTAGTCCTC CACCCCATTC CCCATTGAGT 
CACGGGCATA TCCCCAGTGC CATCGTTCTT CCAAACGCCA CTCACGACTA CAATACATCC 
TTCTCCAACT CGAATGCCCA CAAAGCCGAA AAGAAGCTTC AGAGCATGGA TCACCCTCTT 
ACGAAGTCAT CCAGTAAGCG GGAGCACCGG CGGTCTGTGG ATTCCAGGAA TACTCTCAAT 
GATCTCCTGA AGCATCTAAA TGACCCAAAC AGTAACCCCA AAGCCATCCT GGGAGAGATC 
CATATGGCTC ATCAAACCCT CATGCTGGAC CCGGTGGGAC CAATGGCTGA GGTCCCACCC 
AAGGTCCCTA ACCGGGAGGC ATCTCTATAC TCCCCTCCCT CCACACTCCC CAGAAATAGT 
CCAACCAAGA GAGTAGATGT CCCCACCACT CCTGGGGTGC CAATGACTTC TCTGGAAAGA 
CAAAGGGGTT ATCACAAAAA TTCCTCCCAG AGGCACTCTA TATCTGCCGT GCCTAAAAAC 
TTAAACTCAC CAAATGGTGT TTTGTTATCT AGACAGCCGA GTATGAACCG TGGAGGCTAT 
ATGCCCACCC CAACAGGGGC GAAGGTGGAC TATATTCAGG GGACACCGGT GAGTGTTCAT 
CTGCAGCCCT CCCTCTCCAG ACAGAGCAGC TATACCAGTA ATGGCACCCT CCCCAGGACG 
GGACTAAAGA GGACACCATC CTTAAAACCT GATGTGCCAC CAAAGCCTTC CTTTGTTCCG 
CAAACCACAT CTGTCAGACC ACTGAACAAG TACACGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172490.1
CDS	830..4051
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_017317001.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317001.2

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG 
GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC 
TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA 
GGTATCCCAA AGGGTGTACG GTGGGAAGTC CAGTCTGGAG AATCCAATCA GATGGTCCAC 
ATGAATGTCC TCATCACCTG CGTGTTTGCC GCTTTTGTCT TGGGCGCGTT CATCGCAGGA 
GTGGCCGTGT ACTGCTACCG TGACATGTTC GTTCGGAAGA ACAGAAAGAT CCATAAAGAC 
GCAGAATCCG CCCAGTCGTG CACAGACTCC AGCGGAAGCT TCGCCAAGCT GAACGGCCTC 
TTTGACAGCC CCGTCAAGGA ATACCAGCAG AACATTGATT CTCCCAAACT CTACAGCAAC 
CTGCTGACCA GTCGGAAGGA ACTGCCACCA AACACGGATA CAAAGTCCAT GGCCGTGGAC 
CACAGAGGCC AGCCTCCCGA GCTGGCTGCT CTCCCCACGC CGGAATCCAC ACCTGTCCTC 
CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCTGAGA AGGCCCACAG CCACGGTGCT 
TCAAGGAAAG AACACCCCCA GTTTTTTCCT TCTAGTCCTC CACCCCATTC CCCATTGAGT 
CACGGGCATA TCCCCAGTGC CATCGTTCTT CCAAACGCCA CTCACGACTA CAATACATCC 
TTCTCCAACT CGAATGCCCA CAAAGCCGAA AAGAAGCTTC AGAGCATGGA TCACCCTCTT 
ACGAAGTCAT CCAGTAAGCG GGAGCACCGG CGGTCTGTGG ATTCCAGGAA TACTCTCAAT 
GATCTCCTGA AGCATCTAAA TGACCCAAAC AGTAACCCCA AAGCCATCCT GGGAGAGATC 
CATATGGCTC ATCAAACCCT CATGCTGGAC CCGGTGGGAC CAATGGCTGA GGTCCCACCC 
AAGGTCCCTA ACCGGGAGGC ATCTCTATAC TCCCCTCCCT CCACACTCCC CAGAAATAGT 
CCAACCAAGA GAGTAGATGT CCCCACCACT CCTGGGGTGC CAATGACTTC TCTGGAAAGA 
CAAAGGGGTT ATCACAAAAA TTCCTCCCAG AGGCACTCTA TATCTGCCGT GCCTAAAAAC 
TTAAACTCAC CAAATGGTGT TTTGTTATCT AGACAGCCGA GTATGAACCG TGGAGGCTAT 
ATGCCCACCC CAACAGGGGC GAAGGTGGAC TATATTCAGG GGACACCGGT GAGTGTTCAT 
CTGCAGCCCT CCCTCTCCAG ACAGAGCAGC TATACCAGTA ATGGCACCCT CCCCAGGACG 
GGACTAAAGA GGACACCATC CTTAAAACCT GATGTGCCAC CAAAGCCTTC CTTTGTTCCG 
CAAACCACAT CTGTCAGACC ACTGAACAAG TACACGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_017317012.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172501.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OMu20528
Clone ID Related Accession (Same CDS sequence)	XM_017317012.2 , XM_030249363.1 , XM_011239427.3 , XM_030249361.1 , XM_017317012.1 , XM_011239427.2 , NM_199240.3
Accession Version	XM_017317012.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317012.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT 
GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA 
TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC 
CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA 
GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA 
GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC 
CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC 
AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG 
GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC 
CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT 
GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG 
AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA 
TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT 
CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC 
AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG 
ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA 
CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT 
AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA 
AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA 
AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC 
TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT 
CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG 
ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT 
CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172501.1
CDS	830..3994
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_017317012.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317012.2

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT 
GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA 
TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC 
CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA 
GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA 
GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC 
CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC 
AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG 
GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC 
CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT 
GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG 
AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA 
TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT 
CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC 
AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG 
ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA 
CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT 
AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA 
AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA 
AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC 
TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT 
CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG 
ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT 
CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_030249363.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105223.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OMu20528
Clone ID Related Accession (Same CDS sequence)	XM_017317012.2 , XM_030249363.1 , XM_011239427.3 , XM_030249361.1 , XM_017317012.1 , XM_011239427.2 , NM_199240.3
Accession Version	XM_030249363.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT 
GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA 
TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC 
CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA 
GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA 
GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC 
CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC 
AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG 
GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC 
CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT 
GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG 
AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA 
TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT 
CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC 
AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG 
ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA 
CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT 
AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA 
AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA 
AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC 
TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT 
CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG 
ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT 
CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105223.1
CDS	394..3558
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_030249363.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249363.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT 
GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA 
TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC 
CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA 
GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA 
GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC 
CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC 
AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG 
GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC 
CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT 
GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG 
AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA 
TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT 
CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC 
AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG 
ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA 
CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT 
AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA 
AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA 
AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC 
TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT 
CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG 
ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT 
CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_011239427.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237729.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OMu20528
Clone ID Related Accession (Same CDS sequence)	XM_017317012.2 , XM_030249363.1 , XM_011239427.3 , XM_030249361.1 , XM_017317012.1 , XM_011239427.2 , NM_199240.3
Accession Version	XM_011239427.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011239427.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT 
GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA 
TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC 
CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA 
GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA 
GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC 
CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC 
AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG 
GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC 
CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT 
GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG 
AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA 
TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT 
CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC 
AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG 
ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA 
CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT 
AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA 
AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA 
AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC 
TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT 
CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG 
ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT 
CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237729.1
CDS	605..3769
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_011239427.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239427.3

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT 
GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA 
TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC 
CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA 
GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA 
GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC 
CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC 
AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG 
GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC 
CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT 
GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG 
AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA 
TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT 
CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC 
AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG 
ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA 
CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT 
AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA 
AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA 
AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC 
TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT 
CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG 
ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT 
CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_030249361.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105221.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X18, mRNA.

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CloneID	OMu20528
Clone ID Related Accession (Same CDS sequence)	XM_017317012.2 , XM_030249363.1 , XM_011239427.3 , XM_030249361.1 , XM_017317012.1 , XM_011239427.2 , NM_199240.3
Accession Version	XM_030249361.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT 
GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA 
TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC 
CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA 
GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA 
GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC 
CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC 
AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG 
GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC 
CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT 
GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG 
AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA 
TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT 
CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC 
AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG 
ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA 
CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT 
AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA 
AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA 
AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC 
TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT 
CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG 
ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT 
CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105221.1
CDS	8748..11912
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_030249361.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249361.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT 
GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA 
TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC 
CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA 
GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA 
GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC 
CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC 
AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG 
GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC 
CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT 
GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG 
AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA 
TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT 
CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC 
AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG 
ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA 
CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT 
AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA 
AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA 
AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC 
TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT 
CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG 
ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT 
CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_030249347.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105207.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMu20556
Clone ID Related Accession (Same CDS sequence)	NM_001291000.1 , XM_030249347.1 , XM_030249351.1
Accession Version	XM_030249347.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG 
GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC 
TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA 
GGTGTACGGT GGGAAGTCCA GTCTGGAGAA TCCAATCAGA TGGTCCACAT GAATGTCCTC 
ATCACCTGCG TGTTTGCCGC TTTTGTCTTG GGCGCGTTCA TCGCAGGAGT GGCCGTGTAC 
TGCTACCGTG ACATGTTCGT TCGGAAGAAC AGAAAGATCC ATAAAGACGC AGAATCCGCC 
CAGTCGTGCA CAGACTCCAG CGGAAGCTTC GCCAAGCTGA ACGGCCTCTT TGACAGCCCC 
GTCAAGGAAT ACCAGCAGAA CATTGATTCT CCCAAACTCT ACAGCAACCT GCTGACCAGT 
CGGAAGGAAC TGCCACCAAA CACGGATACA AAGTCCATGG CCGTGGACCA CAGAGGCCAG 
CCTCCCGAGC TGGCTGCTCT CCCCACGCCG GAATCCACAC CTGTCCTCCA CCAGAAGACC 
CTGCAGGCCA TGAAGAGCCA CTCTGAGAAG GCCCACAGCC ACGGTGCTTC AAGGAAAGAA 
CACCCCCAGT TTTTTCCTTC TAGTCCTCCA CCCCATTCCC CATTGAGTCA CGGGCATATC 
CCCAGTGCCA TCGTTCTTCC AAACGCCACT CACGACTACA ATACATCCTT CTCCAACTCG 
AATGCCCACA AAGCCGAAAA GAAGCTTCAG AGCATGGATC ACCCTCTTAC GAAGTCATCC 
AGTAAGCGGG AGCACCGGCG GTCTGTGGAT TCCAGGAATA CTCTCAATGA TCTCCTGAAG 
CATCTAAATG ACCCAAACAG TAACCCCAAA GCCATCCTGG GAGAGATCCA TATGGCTCAT 
CAAACCCTCA TGCTGGACCC GGTGGGACCA ATGGCTGAGG TCCCACCCAA GGTCCCTAAC 
CGGGAGGCAT CTCTATACTC CCCTCCCTCC ACACTCCCCA GAAATAGTCC AACCAAGAGA 
GTAGATGTCC CCACCACTCC TGGGGTGCCA ATGACTTCTC TGGAAAGACA AAGGGGTTAT 
CACAAAAATT CCTCCCAGAG GCACTCTATA TCTGCCGTGC CTAAAAACTT AAACTCACCA 
AATGGTGTTT TGTTATCTAG ACAGCCGAGT ATGAACCGTG GAGGCTATAT GCCCACCCCA 
ACAGGGGCGA AGGTGGACTA TATTCAGGGG ACACCGGTGA GTGTTCATCT GCAGCCCTCC 
CTCTCCAGAC AGAGCAGCTA TACCAGTAAT GGCACCCTCC CCAGGACGGG ACTAAAGAGG 
ACACCATCCT TAAAACCTGA TGTGCCACCA AAGCCTTCCT TTGTTCCGCA AACCACATCT 
GTCAGACCAC TGAACAAGTA CACGTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105207.1
CDS	8748..11957
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_030249347.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249347.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG 
GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC 
TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA 
GGTGTACGGT GGGAAGTCCA GTCTGGAGAA TCCAATCAGA TGGTCCACAT GAATGTCCTC 
ATCACCTGCG TGTTTGCCGC TTTTGTCTTG GGCGCGTTCA TCGCAGGAGT GGCCGTGTAC 
TGCTACCGTG ACATGTTCGT TCGGAAGAAC AGAAAGATCC ATAAAGACGC AGAATCCGCC 
CAGTCGTGCA CAGACTCCAG CGGAAGCTTC GCCAAGCTGA ACGGCCTCTT TGACAGCCCC 
GTCAAGGAAT ACCAGCAGAA CATTGATTCT CCCAAACTCT ACAGCAACCT GCTGACCAGT 
CGGAAGGAAC TGCCACCAAA CACGGATACA AAGTCCATGG CCGTGGACCA CAGAGGCCAG 
CCTCCCGAGC TGGCTGCTCT CCCCACGCCG GAATCCACAC CTGTCCTCCA CCAGAAGACC 
CTGCAGGCCA TGAAGAGCCA CTCTGAGAAG GCCCACAGCC ACGGTGCTTC AAGGAAAGAA 
CACCCCCAGT TTTTTCCTTC TAGTCCTCCA CCCCATTCCC CATTGAGTCA CGGGCATATC 
CCCAGTGCCA TCGTTCTTCC AAACGCCACT CACGACTACA ATACATCCTT CTCCAACTCG 
AATGCCCACA AAGCCGAAAA GAAGCTTCAG AGCATGGATC ACCCTCTTAC GAAGTCATCC 
AGTAAGCGGG AGCACCGGCG GTCTGTGGAT TCCAGGAATA CTCTCAATGA TCTCCTGAAG 
CATCTAAATG ACCCAAACAG TAACCCCAAA GCCATCCTGG GAGAGATCCA TATGGCTCAT 
CAAACCCTCA TGCTGGACCC GGTGGGACCA ATGGCTGAGG TCCCACCCAA GGTCCCTAAC 
CGGGAGGCAT CTCTATACTC CCCTCCCTCC ACACTCCCCA GAAATAGTCC AACCAAGAGA 
GTAGATGTCC CCACCACTCC TGGGGTGCCA ATGACTTCTC TGGAAAGACA AAGGGGTTAT 
CACAAAAATT CCTCCCAGAG GCACTCTATA TCTGCCGTGC CTAAAAACTT AAACTCACCA 
AATGGTGTTT TGTTATCTAG ACAGCCGAGT ATGAACCGTG GAGGCTATAT GCCCACCCCA 
ACAGGGGCGA AGGTGGACTA TATTCAGGGG ACACCGGTGA GTGTTCATCT GCAGCCCTCC 
CTCTCCAGAC AGAGCAGCTA TACCAGTAAT GGCACCCTCC CCAGGACGGG ACTAAAGAGG 
ACACCATCCT TAAAACCTGA TGTGCCACCA AAGCCTTCCT TTGTTCCGCA AACCACATCT 
GTCAGACCAC TGAACAAGTA CACGTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_030249351.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105211.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu20556
Clone ID Related Accession (Same CDS sequence)	NM_001291000.1 , XM_030249347.1 , XM_030249351.1
Accession Version	XM_030249351.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG 
GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC 
TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA 
GGTGTACGGT GGGAAGTCCA GTCTGGAGAA TCCAATCAGA TGGTCCACAT GAATGTCCTC 
ATCACCTGCG TGTTTGCCGC TTTTGTCTTG GGCGCGTTCA TCGCAGGAGT GGCCGTGTAC 
TGCTACCGTG ACATGTTCGT TCGGAAGAAC AGAAAGATCC ATAAAGACGC AGAATCCGCC 
CAGTCGTGCA CAGACTCCAG CGGAAGCTTC GCCAAGCTGA ACGGCCTCTT TGACAGCCCC 
GTCAAGGAAT ACCAGCAGAA CATTGATTCT CCCAAACTCT ACAGCAACCT GCTGACCAGT 
CGGAAGGAAC TGCCACCAAA CACGGATACA AAGTCCATGG CCGTGGACCA CAGAGGCCAG 
CCTCCCGAGC TGGCTGCTCT CCCCACGCCG GAATCCACAC CTGTCCTCCA CCAGAAGACC 
CTGCAGGCCA TGAAGAGCCA CTCTGAGAAG GCCCACAGCC ACGGTGCTTC AAGGAAAGAA 
CACCCCCAGT TTTTTCCTTC TAGTCCTCCA CCCCATTCCC CATTGAGTCA CGGGCATATC 
CCCAGTGCCA TCGTTCTTCC AAACGCCACT CACGACTACA ATACATCCTT CTCCAACTCG 
AATGCCCACA AAGCCGAAAA GAAGCTTCAG AGCATGGATC ACCCTCTTAC GAAGTCATCC 
AGTAAGCGGG AGCACCGGCG GTCTGTGGAT TCCAGGAATA CTCTCAATGA TCTCCTGAAG 
CATCTAAATG ACCCAAACAG TAACCCCAAA GCCATCCTGG GAGAGATCCA TATGGCTCAT 
CAAACCCTCA TGCTGGACCC GGTGGGACCA ATGGCTGAGG TCCCACCCAA GGTCCCTAAC 
CGGGAGGCAT CTCTATACTC CCCTCCCTCC ACACTCCCCA GAAATAGTCC AACCAAGAGA 
GTAGATGTCC CCACCACTCC TGGGGTGCCA ATGACTTCTC TGGAAAGACA AAGGGGTTAT 
CACAAAAATT CCTCCCAGAG GCACTCTATA TCTGCCGTGC CTAAAAACTT AAACTCACCA 
AATGGTGTTT TGTTATCTAG ACAGCCGAGT ATGAACCGTG GAGGCTATAT GCCCACCCCA 
ACAGGGGCGA AGGTGGACTA TATTCAGGGG ACACCGGTGA GTGTTCATCT GCAGCCCTCC 
CTCTCCAGAC AGAGCAGCTA TACCAGTAAT GGCACCCTCC CCAGGACGGG ACTAAAGAGG 
ACACCATCCT TAAAACCTGA TGTGCCACCA AAGCCTTCCT TTGTTCCGCA AACCACATCT 
GTCAGACCAC TGAACAAGTA CACGTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105211.1
CDS	832..4041
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_030249351.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249351.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG 
GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC 
TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA 
GGTGTACGGT GGGAAGTCCA GTCTGGAGAA TCCAATCAGA TGGTCCACAT GAATGTCCTC 
ATCACCTGCG TGTTTGCCGC TTTTGTCTTG GGCGCGTTCA TCGCAGGAGT GGCCGTGTAC 
TGCTACCGTG ACATGTTCGT TCGGAAGAAC AGAAAGATCC ATAAAGACGC AGAATCCGCC 
CAGTCGTGCA CAGACTCCAG CGGAAGCTTC GCCAAGCTGA ACGGCCTCTT TGACAGCCCC 
GTCAAGGAAT ACCAGCAGAA CATTGATTCT CCCAAACTCT ACAGCAACCT GCTGACCAGT 
CGGAAGGAAC TGCCACCAAA CACGGATACA AAGTCCATGG CCGTGGACCA CAGAGGCCAG 
CCTCCCGAGC TGGCTGCTCT CCCCACGCCG GAATCCACAC CTGTCCTCCA CCAGAAGACC 
CTGCAGGCCA TGAAGAGCCA CTCTGAGAAG GCCCACAGCC ACGGTGCTTC AAGGAAAGAA 
CACCCCCAGT TTTTTCCTTC TAGTCCTCCA CCCCATTCCC CATTGAGTCA CGGGCATATC 
CCCAGTGCCA TCGTTCTTCC AAACGCCACT CACGACTACA ATACATCCTT CTCCAACTCG 
AATGCCCACA AAGCCGAAAA GAAGCTTCAG AGCATGGATC ACCCTCTTAC GAAGTCATCC 
AGTAAGCGGG AGCACCGGCG GTCTGTGGAT TCCAGGAATA CTCTCAATGA TCTCCTGAAG 
CATCTAAATG ACCCAAACAG TAACCCCAAA GCCATCCTGG GAGAGATCCA TATGGCTCAT 
CAAACCCTCA TGCTGGACCC GGTGGGACCA ATGGCTGAGG TCCCACCCAA GGTCCCTAAC 
CGGGAGGCAT CTCTATACTC CCCTCCCTCC ACACTCCCCA GAAATAGTCC AACCAAGAGA 
GTAGATGTCC CCACCACTCC TGGGGTGCCA ATGACTTCTC TGGAAAGACA AAGGGGTTAT 
CACAAAAATT CCTCCCAGAG GCACTCTATA TCTGCCGTGC CTAAAAACTT AAACTCACCA 
AATGGTGTTT TGTTATCTAG ACAGCCGAGT ATGAACCGTG GAGGCTATAT GCCCACCCCA 
ACAGGGGCGA AGGTGGACTA TATTCAGGGG ACACCGGTGA GTGTTCATCT GCAGCCCTCC 
CTCTCCAGAC AGAGCAGCTA TACCAGTAAT GGCACCCTCC CCAGGACGGG ACTAAAGAGG 
ACACCATCCT TAAAACCTGA TGTGCCACCA AAGCCTTCCT TTGTTCCGCA AACCACATCT 
GTCAGACCAC TGAACAAGTA CACGTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_030249335.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105195.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu30226
Clone ID Related Accession (Same CDS sequence)	XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
Accession Version	XM_030249335.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105195.1
CDS	471..3731
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_030249335.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249335.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_006499111.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499174.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu30226
Clone ID Related Accession (Same CDS sequence)	XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
Accession Version	XM_006499111.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499111.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499174.1
CDS	830..4090
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_006499111.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499111.4

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_006499112.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499175.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu30226
Clone ID Related Accession (Same CDS sequence)	XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
Accession Version	XM_006499112.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499112.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499175.1
CDS	485..3745
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_006499112.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499112.4

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_030249334.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105194.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu30226
Clone ID Related Accession (Same CDS sequence)	XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
Accession Version	XM_030249334.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105194.1
CDS	444..3704
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_030249334.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249334.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_006499113.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499176.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X5, mRNA.

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CloneID	OMu30226
Clone ID Related Accession (Same CDS sequence)	XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
Accession Version	XM_006499113.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499113.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499176.1
CDS	8748..12008
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_006499113.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499113.4

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_006499114.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499177.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X6, mRNA.

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CloneID	OMu30226
Clone ID Related Accession (Same CDS sequence)	XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
Accession Version	XM_006499114.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499114.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499177.1
CDS	839..4099
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_006499114.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499114.4

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_017316995.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172484.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu72911
Clone ID Related Accession (Same CDS sequence)	XM_017316995.2 , XM_017316995.1
Accession Version	XM_017316995.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316995.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTGTACGGTG GGAAGTCCAG 
TCTGGAGAAT CCAATCAGAT GGTCCACATG AATGTCCTCA TCACCTGCGT GTTTGCCGCT 
TTTGTCTTGG GCGCGTTCAT CGCAGGAGTG GCCGTGTACT GCTACCGTGA CATGTTCGTT 
CGGAAGAACA GAAAGATCCA TAAAGACGCA GAATCCGCCC AGTCGTGCAC AGACTCCAGC 
GGAAGCTTCG CCAAGCTGAA CGGCCTCTTT GACAGCCCCG TCAAGGAATA CCAGCAGAAC 
ATTGATTCTC CCAAACTCTA CAGCAACCTG CTGACCAGTC GGAAGGAACT GCCACCAAAC 
ACGGATACAA AGTCCATGGC CGTGGACCAC AGAGGCCAGC CTCCCGAGCT GGCTGCTCTC 
CCCACGCCGG AATCCACACC TGTCCTCCAC CAGAAGACCC TGCAGGCCAT GAAGAGCCAC 
TCTGAGAAGG CCCACAGCCA CGGTGCTTCA AGGAAAGAAC ACCCCCAGTT TTTTCCTTCT 
AGTCCTCCAC CCCATTCCCC ATTGAGTCAC GGGCATATCC CCAGTGCCAT CGTTCTTCCA 
AACGCCACTC ACGACTACAA TACATCCTTC TCCAACTCGA ATGCCCACAA AGCCGAAAAG 
AAGCTTCAGA GCATGGATCA CCCTCTTACG AAGTCATCCA GTAAGCGGGA GCACCGGCGG 
TCTGTGGATT CCAGGAATAC TCTCAATGAT CTCCTGAAGC ATCTAAATGA CCCAAACAGT 
AACCCCAAAG CCATCCTGGG AGAGATCCAT ATGGCTCATC AAACCCTCAT GCTGGACCCG 
GTGGGACCAA TGGCTGAGGT CCCACCCAAG GTCCCTAACC GGGAGGCATC TCTATACTCC 
CCTCCCTCCA CACTCCCCAG AAATAGTCCA ACCAAGAGAG TAGATGTCCC CACCACTCCT 
GGGGTGCCAA TGACTTCTCT GGAAAGACAA AGGGGTTATC ACAAAAATTC CTCCCAGAGG 
CACTCTATAT CTGCCGTGCC TAAAAACTTA AACTCACCAA ATGGTGTTTT GTTATCTAGA 
CAGCCGAGTA TGAACCGTGG AGGCTATATG CCCACCCCAA CAGGGGCGAA GGTGGACTAT 
ATTCAGGGGA CACCGGTGAG TGTTCATCTG CAGCCCTCCC TCTCCAGACA GAGCAGCTAT 
ACCAGTAATG GCACCCTCCC CAGGACGGGA CTAAAGAGGA CACCATCCTT AAAACCTGAT 
GTGCCACCAA AGCCTTCCTT TGTTCCGCAA ACCACATCTG TCAGACCACT GAACAAGTAC 
ACGTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172484.1
CDS	830..4078
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_017316995.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316995.2

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTGTACGGTG GGAAGTCCAG 
TCTGGAGAAT CCAATCAGAT GGTCCACATG AATGTCCTCA TCACCTGCGT GTTTGCCGCT 
TTTGTCTTGG GCGCGTTCAT CGCAGGAGTG GCCGTGTACT GCTACCGTGA CATGTTCGTT 
CGGAAGAACA GAAAGATCCA TAAAGACGCA GAATCCGCCC AGTCGTGCAC AGACTCCAGC 
GGAAGCTTCG CCAAGCTGAA CGGCCTCTTT GACAGCCCCG TCAAGGAATA CCAGCAGAAC 
ATTGATTCTC CCAAACTCTA CAGCAACCTG CTGACCAGTC GGAAGGAACT GCCACCAAAC 
ACGGATACAA AGTCCATGGC CGTGGACCAC AGAGGCCAGC CTCCCGAGCT GGCTGCTCTC 
CCCACGCCGG AATCCACACC TGTCCTCCAC CAGAAGACCC TGCAGGCCAT GAAGAGCCAC 
TCTGAGAAGG CCCACAGCCA CGGTGCTTCA AGGAAAGAAC ACCCCCAGTT TTTTCCTTCT 
AGTCCTCCAC CCCATTCCCC ATTGAGTCAC GGGCATATCC CCAGTGCCAT CGTTCTTCCA 
AACGCCACTC ACGACTACAA TACATCCTTC TCCAACTCGA ATGCCCACAA AGCCGAAAAG 
AAGCTTCAGA GCATGGATCA CCCTCTTACG AAGTCATCCA GTAAGCGGGA GCACCGGCGG 
TCTGTGGATT CCAGGAATAC TCTCAATGAT CTCCTGAAGC ATCTAAATGA CCCAAACAGT 
AACCCCAAAG CCATCCTGGG AGAGATCCAT ATGGCTCATC AAACCCTCAT GCTGGACCCG 
GTGGGACCAA TGGCTGAGGT CCCACCCAAG GTCCCTAACC GGGAGGCATC TCTATACTCC 
CCTCCCTCCA CACTCCCCAG AAATAGTCCA ACCAAGAGAG TAGATGTCCC CACCACTCCT 
GGGGTGCCAA TGACTTCTCT GGAAAGACAA AGGGGTTATC ACAAAAATTC CTCCCAGAGG 
CACTCTATAT CTGCCGTGCC TAAAAACTTA AACTCACCAA ATGGTGTTTT GTTATCTAGA 
CAGCCGAGTA TGAACCGTGG AGGCTATATG CCCACCCCAA CAGGGGCGAA GGTGGACTAT 
ATTCAGGGGA CACCGGTGAG TGTTCATCTG CAGCCCTCCC TCTCCAGACA GAGCAGCTAT 
ACCAGTAATG GCACCCTCCC CAGGACGGGA CTAAAGAGGA CACCATCCTT AAAACCTGAT 
GTGCCACCAA AGCCTTCCTT TGTTCCGCAA ACCACATCTG TCAGACCACT GAACAAGTAC 
ACGTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_030249357.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105217.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OMu72912
Clone ID Related Accession (Same CDS sequence)	XM_030249357.1 , XM_017317010.1 , XM_017317010.2
Accession Version	XM_030249357.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGACA TGGAGGTATC CTCATCTTCT GTTACCACTG TGGCAAGTAG CCCAGAAATT 
ACATCTAAAG TGATTGATAC CTGGAGACCT AAACTGACGA GCTCCCGGAA ATTTGTAGTT 
CAAGATGACC CAAATACTTC TGATTTTACT GATACTATAT CAGGTATCCC AAAGGGTGTA 
CGGTGGGAAG TCCAGTCTGG AGAATCCAAT CAGATGGTCC ACATGAATGT CCTCATCACC 
TGCGTGTTTG CCGCTTTTGT CTTGGGCGCG TTCATCGCAG GAGTGGCCGT GTACTGCTAC 
CGTGACATGT TCGTTCGGAA GAACAGAAAG ATCCATAAAG ACGCAGAATC CGCCCAGTCG 
TGCACAGACT CCAGCGGAAG CTTCGCCAAG CTGAACGGCC TCTTTGACAG CCCCGTCAAG 
GAATACCAGC AGAACATTGA TTCTCCCAAA CTCTACAGCA ACCTGCTGAC CAGTCGGAAG 
GAACTGCCAC CAAACACGGA TACAAAGTCC ATGGCCGTGG ACCACAGAGG CCAGCCTCCC 
GAGCTGGCTG CTCTCCCCAC GCCGGAATCC ACACCTGTCC TCCACCAGAA GACCCTGCAG 
GCCATGAAGA GCCACTCTGA GAAGGCCCAC AGCCACGGTG CTTCAAGGAA AGAACACCCC 
CAGTTTTTTC CTTCTAGTCC TCCACCCCAT TCCCCATTGA GTCACGGGCA TATCCCCAGT 
GCCATCGTTC TTCCAAACGC CACTCACGAC TACAATACAT CCTTCTCCAA CTCGAATGCC 
CACAAAGCCG AAAAGAAGCT TCAGAGCATG GATCACCCTC TTACGAAGTC ATCCAGTAAG 
CGGGAGCACC GGCGGTCTGT GGATTCCAGG AATACTCTCA ATGATCTCCT GAAGCATCTA 
AATGACCCAA ACAGTAACCC CAAAGCCATC CTGGGAGAGA TCCATATGGC TCATCAAACC 
CTCATGCTGG ACCCGGTGGG ACCAATGGCT GAGGTCCCAC CCAAGGTCCC TAACCGGGAG 
GCATCTCTAT ACTCCCCTCC CTCCACACTC CCCAGAAATA GTCCAACCAA GAGAGTAGAT 
GTCCCCACCA CTCCTGGGGT GCCAATGACT TCTCTGGAAA GACAAAGGGG TTATCACAAA 
AATTCCTCCC AGAGGCACTC TATATCTGCC GTGCCTAAAA ACTTAAACTC ACCAAATGGT 
GTTTTGTTAT CTAGACAGCC GAGTATGAAC CGTGGAGGCT ATATGCCCAC CCCAACAGGG 
GCGAAGGTGG ACTATATTCA GGGGACACCG GTGAGTGTTC ATCTGCAGCC CTCCCTCTCC 
AGACAGAGCA GCTATACCAG TAATGGCACC CTCCCCAGGA CGGGACTAAA GAGGACACCA 
TCCTTAAAAC CTGATGTGCC ACCAAAGCCT TCCTTTGTTC CGCAAACCAC ATCTGTCAGA 
CCACTGAACA AGTACACGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105217.1
CDS	8748..11951
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_030249357.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249357.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGACA TGGAGGTATC CTCATCTTCT GTTACCACTG TGGCAAGTAG CCCAGAAATT 
ACATCTAAAG TGATTGATAC CTGGAGACCT AAACTGACGA GCTCCCGGAA ATTTGTAGTT 
CAAGATGACC CAAATACTTC TGATTTTACT GATACTATAT CAGGTATCCC AAAGGGTGTA 
CGGTGGGAAG TCCAGTCTGG AGAATCCAAT CAGATGGTCC ACATGAATGT CCTCATCACC 
TGCGTGTTTG CCGCTTTTGT CTTGGGCGCG TTCATCGCAG GAGTGGCCGT GTACTGCTAC 
CGTGACATGT TCGTTCGGAA GAACAGAAAG ATCCATAAAG ACGCAGAATC CGCCCAGTCG 
TGCACAGACT CCAGCGGAAG CTTCGCCAAG CTGAACGGCC TCTTTGACAG CCCCGTCAAG 
GAATACCAGC AGAACATTGA TTCTCCCAAA CTCTACAGCA ACCTGCTGAC CAGTCGGAAG 
GAACTGCCAC CAAACACGGA TACAAAGTCC ATGGCCGTGG ACCACAGAGG CCAGCCTCCC 
GAGCTGGCTG CTCTCCCCAC GCCGGAATCC ACACCTGTCC TCCACCAGAA GACCCTGCAG 
GCCATGAAGA GCCACTCTGA GAAGGCCCAC AGCCACGGTG CTTCAAGGAA AGAACACCCC 
CAGTTTTTTC CTTCTAGTCC TCCACCCCAT TCCCCATTGA GTCACGGGCA TATCCCCAGT 
GCCATCGTTC TTCCAAACGC CACTCACGAC TACAATACAT CCTTCTCCAA CTCGAATGCC 
CACAAAGCCG AAAAGAAGCT TCAGAGCATG GATCACCCTC TTACGAAGTC ATCCAGTAAG 
CGGGAGCACC GGCGGTCTGT GGATTCCAGG AATACTCTCA ATGATCTCCT GAAGCATCTA 
AATGACCCAA ACAGTAACCC CAAAGCCATC CTGGGAGAGA TCCATATGGC TCATCAAACC 
CTCATGCTGG ACCCGGTGGG ACCAATGGCT GAGGTCCCAC CCAAGGTCCC TAACCGGGAG 
GCATCTCTAT ACTCCCCTCC CTCCACACTC CCCAGAAATA GTCCAACCAA GAGAGTAGAT 
GTCCCCACCA CTCCTGGGGT GCCAATGACT TCTCTGGAAA GACAAAGGGG TTATCACAAA 
AATTCCTCCC AGAGGCACTC TATATCTGCC GTGCCTAAAA ACTTAAACTC ACCAAATGGT 
GTTTTGTTAT CTAGACAGCC GAGTATGAAC CGTGGAGGCT ATATGCCCAC CCCAACAGGG 
GCGAAGGTGG ACTATATTCA GGGGACACCG GTGAGTGTTC ATCTGCAGCC CTCCCTCTCC 
AGACAGAGCA GCTATACCAG TAATGGCACC CTCCCCAGGA CGGGACTAAA GAGGACACCA 
TCCTTAAAAC CTGATGTGCC ACCAAAGCCT TCCTTTGTTC CGCAAACCAC ATCTGTCAGA 
CCACTGAACA AGTACACGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_017317010.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172499.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OMu72912
Clone ID Related Accession (Same CDS sequence)	XM_030249357.1 , XM_017317010.1 , XM_017317010.2
Accession Version	XM_017317010.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317010.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGACA TGGAGGTATC CTCATCTTCT GTTACCACTG TGGCAAGTAG CCCAGAAATT 
ACATCTAAAG TGATTGATAC CTGGAGACCT AAACTGACGA GCTCCCGGAA ATTTGTAGTT 
CAAGATGACC CAAATACTTC TGATTTTACT GATACTATAT CAGGTATCCC AAAGGGTGTA 
CGGTGGGAAG TCCAGTCTGG AGAATCCAAT CAGATGGTCC ACATGAATGT CCTCATCACC 
TGCGTGTTTG CCGCTTTTGT CTTGGGCGCG TTCATCGCAG GAGTGGCCGT GTACTGCTAC 
CGTGACATGT TCGTTCGGAA GAACAGAAAG ATCCATAAAG ACGCAGAATC CGCCCAGTCG 
TGCACAGACT CCAGCGGAAG CTTCGCCAAG CTGAACGGCC TCTTTGACAG CCCCGTCAAG 
GAATACCAGC AGAACATTGA TTCTCCCAAA CTCTACAGCA ACCTGCTGAC CAGTCGGAAG 
GAACTGCCAC CAAACACGGA TACAAAGTCC ATGGCCGTGG ACCACAGAGG CCAGCCTCCC 
GAGCTGGCTG CTCTCCCCAC GCCGGAATCC ACACCTGTCC TCCACCAGAA GACCCTGCAG 
GCCATGAAGA GCCACTCTGA GAAGGCCCAC AGCCACGGTG CTTCAAGGAA AGAACACCCC 
CAGTTTTTTC CTTCTAGTCC TCCACCCCAT TCCCCATTGA GTCACGGGCA TATCCCCAGT 
GCCATCGTTC TTCCAAACGC CACTCACGAC TACAATACAT CCTTCTCCAA CTCGAATGCC 
CACAAAGCCG AAAAGAAGCT TCAGAGCATG GATCACCCTC TTACGAAGTC ATCCAGTAAG 
CGGGAGCACC GGCGGTCTGT GGATTCCAGG AATACTCTCA ATGATCTCCT GAAGCATCTA 
AATGACCCAA ACAGTAACCC CAAAGCCATC CTGGGAGAGA TCCATATGGC TCATCAAACC 
CTCATGCTGG ACCCGGTGGG ACCAATGGCT GAGGTCCCAC CCAAGGTCCC TAACCGGGAG 
GCATCTCTAT ACTCCCCTCC CTCCACACTC CCCAGAAATA GTCCAACCAA GAGAGTAGAT 
GTCCCCACCA CTCCTGGGGT GCCAATGACT TCTCTGGAAA GACAAAGGGG TTATCACAAA 
AATTCCTCCC AGAGGCACTC TATATCTGCC GTGCCTAAAA ACTTAAACTC ACCAAATGGT 
GTTTTGTTAT CTAGACAGCC GAGTATGAAC CGTGGAGGCT ATATGCCCAC CCCAACAGGG 
GCGAAGGTGG ACTATATTCA GGGGACACCG GTGAGTGTTC ATCTGCAGCC CTCCCTCTCC 
AGACAGAGCA GCTATACCAG TAATGGCACC CTCCCCAGGA CGGGACTAAA GAGGACACCA 
TCCTTAAAAC CTGATGTGCC ACCAAAGCCT TCCTTTGTTC CGCAAACCAC ATCTGTCAGA 
CCACTGAACA AGTACACGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172499.1
CDS	830..4033
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_017317010.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317010.2

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGACA TGGAGGTATC CTCATCTTCT GTTACCACTG TGGCAAGTAG CCCAGAAATT 
ACATCTAAAG TGATTGATAC CTGGAGACCT AAACTGACGA GCTCCCGGAA ATTTGTAGTT 
CAAGATGACC CAAATACTTC TGATTTTACT GATACTATAT CAGGTATCCC AAAGGGTGTA 
CGGTGGGAAG TCCAGTCTGG AGAATCCAAT CAGATGGTCC ACATGAATGT CCTCATCACC 
TGCGTGTTTG CCGCTTTTGT CTTGGGCGCG TTCATCGCAG GAGTGGCCGT GTACTGCTAC 
CGTGACATGT TCGTTCGGAA GAACAGAAAG ATCCATAAAG ACGCAGAATC CGCCCAGTCG 
TGCACAGACT CCAGCGGAAG CTTCGCCAAG CTGAACGGCC TCTTTGACAG CCCCGTCAAG 
GAATACCAGC AGAACATTGA TTCTCCCAAA CTCTACAGCA ACCTGCTGAC CAGTCGGAAG 
GAACTGCCAC CAAACACGGA TACAAAGTCC ATGGCCGTGG ACCACAGAGG CCAGCCTCCC 
GAGCTGGCTG CTCTCCCCAC GCCGGAATCC ACACCTGTCC TCCACCAGAA GACCCTGCAG 
GCCATGAAGA GCCACTCTGA GAAGGCCCAC AGCCACGGTG CTTCAAGGAA AGAACACCCC 
CAGTTTTTTC CTTCTAGTCC TCCACCCCAT TCCCCATTGA GTCACGGGCA TATCCCCAGT 
GCCATCGTTC TTCCAAACGC CACTCACGAC TACAATACAT CCTTCTCCAA CTCGAATGCC 
CACAAAGCCG AAAAGAAGCT TCAGAGCATG GATCACCCTC TTACGAAGTC ATCCAGTAAG 
CGGGAGCACC GGCGGTCTGT GGATTCCAGG AATACTCTCA ATGATCTCCT GAAGCATCTA 
AATGACCCAA ACAGTAACCC CAAAGCCATC CTGGGAGAGA TCCATATGGC TCATCAAACC 
CTCATGCTGG ACCCGGTGGG ACCAATGGCT GAGGTCCCAC CCAAGGTCCC TAACCGGGAG 
GCATCTCTAT ACTCCCCTCC CTCCACACTC CCCAGAAATA GTCCAACCAA GAGAGTAGAT 
GTCCCCACCA CTCCTGGGGT GCCAATGACT TCTCTGGAAA GACAAAGGGG TTATCACAAA 
AATTCCTCCC AGAGGCACTC TATATCTGCC GTGCCTAAAA ACTTAAACTC ACCAAATGGT 
GTTTTGTTAT CTAGACAGCC GAGTATGAAC CGTGGAGGCT ATATGCCCAC CCCAACAGGG 
GCGAAGGTGG ACTATATTCA GGGGACACCG GTGAGTGTTC ATCTGCAGCC CTCCCTCTCC 
AGACAGAGCA GCTATACCAG TAATGGCACC CTCCCCAGGA CGGGACTAAA GAGGACACCA 
TCCTTAAAAC CTGATGTGCC ACCAAAGCCT TCCTTTGTTC CGCAAACCAC ATCTGTCAGA 
CCACTGAACA AGTACACGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_017317021.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172510.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X24, mRNA.

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CloneID	OMu72913
Clone ID Related Accession (Same CDS sequence)	XM_017317021.2 , XM_030249368.1 , XM_017317021.1
Accession Version	XM_017317021.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317021.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGGTGTA CGGTGGGAAG TCCAGTCTGG AGAATCCAAT 
CAGATGGTCC ACATGAATGT CCTCATCACC TGCGTGTTTG CCGCTTTTGT CTTGGGCGCG 
TTCATCGCAG GAGTGGCCGT GTACTGCTAC CGTGACATGT TCGTTCGGAA GAACAGAAAG 
ATCCATAAAG ACGCAGAATC CGCCCAGTCG TGCACAGACT CCAGCGGAAG CTTCGCCAAG 
CTGAACGGCC TCTTTGACAG CCCCGTCAAG GAATACCAGC AGAACATTGA TTCTCCCAAA 
CTCTACAGCA ACCTGCTGAC CAGTCGGAAG GAACTGCCAC CAAACACGGA TACAAAGTCC 
ATGGCCGTGG ACCACAGAGG CCAGCCTCCC GAGCTGGCTG CTCTCCCCAC GCCGGAATCC 
ACACCTGTCC TCCACCAGAA GACCCTGCAG GCCATGAAGA GCCACTCTGA GAAGGCCCAC 
AGCCACGGTG CTTCAAGGAA AGAACACCCC CAGTTTTTTC CTTCTAGTCC TCCACCCCAT 
TCCCCATTGA GTCACGGGCA TATCCCCAGT GCCATCGTTC TTCCAAACGC CACTCACGAC 
TACAATACAT CCTTCTCCAA CTCGAATGCC CACAAAGCCG AAAAGAAGCT TCAGAGCATG 
GATCACCCTC TTACGAAGTC ATCCAGTAAG CGGGAGCACC GGCGGTCTGT GGATTCCAGG 
AATACTCTCA ATGATCTCCT GAAGCATCTA AATGACCCAA ACAGTAACCC CAAAGCCATC 
CTGGGAGAGA TCCATATGGC TCATCAAACC CTCATGCTGG ACCCGGTGGG ACCAATGGCT 
GAGGTCCCAC CCAAGGTCCC TAACCGGGAG GCATCTCTAT ACTCCCCTCC CTCCACACTC 
CCCAGAAATA GTCCAACCAA GAGAGTAGAT GTCCCCACCA CTCCTGGGGT GCCAATGACT 
TCTCTGGAAA GACAAAGGGG TTATCACAAA AATTCCTCCC AGAGGCACTC TATATCTGCC 
GTGCCTAAAA ACTTAAACTC ACCAAATGGT GTTTTGTTAT CTAGACAGCC GAGTATGAAC 
CGTGGAGGCT ATATGCCCAC CCCAACAGGG GCGAAGGTGG ACTATATTCA GGGGACACCG 
GTGAGTGTTC ATCTGCAGCC CTCCCTCTCC AGACAGAGCA GCTATACCAG TAATGGCACC 
CTCCCCAGGA CGGGACTAAA GAGGACACCA TCCTTAAAAC CTGATGTGCC ACCAAAGCCT 
TCCTTTGTTC CGCAAACCAC ATCTGTCAGA CCACTGAACA AGTACACGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172510.1
CDS	830..3883
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_017317021.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317021.2

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGGTGTA CGGTGGGAAG TCCAGTCTGG AGAATCCAAT 
CAGATGGTCC ACATGAATGT CCTCATCACC TGCGTGTTTG CCGCTTTTGT CTTGGGCGCG 
TTCATCGCAG GAGTGGCCGT GTACTGCTAC CGTGACATGT TCGTTCGGAA GAACAGAAAG 
ATCCATAAAG ACGCAGAATC CGCCCAGTCG TGCACAGACT CCAGCGGAAG CTTCGCCAAG 
CTGAACGGCC TCTTTGACAG CCCCGTCAAG GAATACCAGC AGAACATTGA TTCTCCCAAA 
CTCTACAGCA ACCTGCTGAC CAGTCGGAAG GAACTGCCAC CAAACACGGA TACAAAGTCC 
ATGGCCGTGG ACCACAGAGG CCAGCCTCCC GAGCTGGCTG CTCTCCCCAC GCCGGAATCC 
ACACCTGTCC TCCACCAGAA GACCCTGCAG GCCATGAAGA GCCACTCTGA GAAGGCCCAC 
AGCCACGGTG CTTCAAGGAA AGAACACCCC CAGTTTTTTC CTTCTAGTCC TCCACCCCAT 
TCCCCATTGA GTCACGGGCA TATCCCCAGT GCCATCGTTC TTCCAAACGC CACTCACGAC 
TACAATACAT CCTTCTCCAA CTCGAATGCC CACAAAGCCG AAAAGAAGCT TCAGAGCATG 
GATCACCCTC TTACGAAGTC ATCCAGTAAG CGGGAGCACC GGCGGTCTGT GGATTCCAGG 
AATACTCTCA ATGATCTCCT GAAGCATCTA AATGACCCAA ACAGTAACCC CAAAGCCATC 
CTGGGAGAGA TCCATATGGC TCATCAAACC CTCATGCTGG ACCCGGTGGG ACCAATGGCT 
GAGGTCCCAC CCAAGGTCCC TAACCGGGAG GCATCTCTAT ACTCCCCTCC CTCCACACTC 
CCCAGAAATA GTCCAACCAA GAGAGTAGAT GTCCCCACCA CTCCTGGGGT GCCAATGACT 
TCTCTGGAAA GACAAAGGGG TTATCACAAA AATTCCTCCC AGAGGCACTC TATATCTGCC 
GTGCCTAAAA ACTTAAACTC ACCAAATGGT GTTTTGTTAT CTAGACAGCC GAGTATGAAC 
CGTGGAGGCT ATATGCCCAC CCCAACAGGG GCGAAGGTGG ACTATATTCA GGGGACACCG 
GTGAGTGTTC ATCTGCAGCC CTCCCTCTCC AGACAGAGCA GCTATACCAG TAATGGCACC 
CTCCCCAGGA CGGGACTAAA GAGGACACCA TCCTTAAAAC CTGATGTGCC ACCAAAGCCT 
TCCTTTGTTC CGCAAACCAC ATCTGTCAGA CCACTGAACA AGTACACGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_030249368.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105228.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X25, mRNA.

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CloneID	OMu72913
Clone ID Related Accession (Same CDS sequence)	XM_017317021.2 , XM_030249368.1 , XM_017317021.1
Accession Version	XM_030249368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGGTGTA CGGTGGGAAG TCCAGTCTGG AGAATCCAAT 
CAGATGGTCC ACATGAATGT CCTCATCACC TGCGTGTTTG CCGCTTTTGT CTTGGGCGCG 
TTCATCGCAG GAGTGGCCGT GTACTGCTAC CGTGACATGT TCGTTCGGAA GAACAGAAAG 
ATCCATAAAG ACGCAGAATC CGCCCAGTCG TGCACAGACT CCAGCGGAAG CTTCGCCAAG 
CTGAACGGCC TCTTTGACAG CCCCGTCAAG GAATACCAGC AGAACATTGA TTCTCCCAAA 
CTCTACAGCA ACCTGCTGAC CAGTCGGAAG GAACTGCCAC CAAACACGGA TACAAAGTCC 
ATGGCCGTGG ACCACAGAGG CCAGCCTCCC GAGCTGGCTG CTCTCCCCAC GCCGGAATCC 
ACACCTGTCC TCCACCAGAA GACCCTGCAG GCCATGAAGA GCCACTCTGA GAAGGCCCAC 
AGCCACGGTG CTTCAAGGAA AGAACACCCC CAGTTTTTTC CTTCTAGTCC TCCACCCCAT 
TCCCCATTGA GTCACGGGCA TATCCCCAGT GCCATCGTTC TTCCAAACGC CACTCACGAC 
TACAATACAT CCTTCTCCAA CTCGAATGCC CACAAAGCCG AAAAGAAGCT TCAGAGCATG 
GATCACCCTC TTACGAAGTC ATCCAGTAAG CGGGAGCACC GGCGGTCTGT GGATTCCAGG 
AATACTCTCA ATGATCTCCT GAAGCATCTA AATGACCCAA ACAGTAACCC CAAAGCCATC 
CTGGGAGAGA TCCATATGGC TCATCAAACC CTCATGCTGG ACCCGGTGGG ACCAATGGCT 
GAGGTCCCAC CCAAGGTCCC TAACCGGGAG GCATCTCTAT ACTCCCCTCC CTCCACACTC 
CCCAGAAATA GTCCAACCAA GAGAGTAGAT GTCCCCACCA CTCCTGGGGT GCCAATGACT 
TCTCTGGAAA GACAAAGGGG TTATCACAAA AATTCCTCCC AGAGGCACTC TATATCTGCC 
GTGCCTAAAA ACTTAAACTC ACCAAATGGT GTTTTGTTAT CTAGACAGCC GAGTATGAAC 
CGTGGAGGCT ATATGCCCAC CCCAACAGGG GCGAAGGTGG ACTATATTCA GGGGACACCG 
GTGAGTGTTC ATCTGCAGCC CTCCCTCTCC AGACAGAGCA GCTATACCAG TAATGGCACC 
CTCCCCAGGA CGGGACTAAA GAGGACACCA TCCTTAAAAC CTGATGTGCC ACCAAAGCCT 
TCCTTTGTTC CGCAAACCAC ATCTGTCAGA CCACTGAACA AGTACACGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105228.1
CDS	8748..11801
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_030249368.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249368.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGGTGTA CGGTGGGAAG TCCAGTCTGG AGAATCCAAT 
CAGATGGTCC ACATGAATGT CCTCATCACC TGCGTGTTTG CCGCTTTTGT CTTGGGCGCG 
TTCATCGCAG GAGTGGCCGT GTACTGCTAC CGTGACATGT TCGTTCGGAA GAACAGAAAG 
ATCCATAAAG ACGCAGAATC CGCCCAGTCG TGCACAGACT CCAGCGGAAG CTTCGCCAAG 
CTGAACGGCC TCTTTGACAG CCCCGTCAAG GAATACCAGC AGAACATTGA TTCTCCCAAA 
CTCTACAGCA ACCTGCTGAC CAGTCGGAAG GAACTGCCAC CAAACACGGA TACAAAGTCC 
ATGGCCGTGG ACCACAGAGG CCAGCCTCCC GAGCTGGCTG CTCTCCCCAC GCCGGAATCC 
ACACCTGTCC TCCACCAGAA GACCCTGCAG GCCATGAAGA GCCACTCTGA GAAGGCCCAC 
AGCCACGGTG CTTCAAGGAA AGAACACCCC CAGTTTTTTC CTTCTAGTCC TCCACCCCAT 
TCCCCATTGA GTCACGGGCA TATCCCCAGT GCCATCGTTC TTCCAAACGC CACTCACGAC 
TACAATACAT CCTTCTCCAA CTCGAATGCC CACAAAGCCG AAAAGAAGCT TCAGAGCATG 
GATCACCCTC TTACGAAGTC ATCCAGTAAG CGGGAGCACC GGCGGTCTGT GGATTCCAGG 
AATACTCTCA ATGATCTCCT GAAGCATCTA AATGACCCAA ACAGTAACCC CAAAGCCATC 
CTGGGAGAGA TCCATATGGC TCATCAAACC CTCATGCTGG ACCCGGTGGG ACCAATGGCT 
GAGGTCCCAC CCAAGGTCCC TAACCGGGAG GCATCTCTAT ACTCCCCTCC CTCCACACTC 
CCCAGAAATA GTCCAACCAA GAGAGTAGAT GTCCCCACCA CTCCTGGGGT GCCAATGACT 
TCTCTGGAAA GACAAAGGGG TTATCACAAA AATTCCTCCC AGAGGCACTC TATATCTGCC 
GTGCCTAAAA ACTTAAACTC ACCAAATGGT GTTTTGTTAT CTAGACAGCC GAGTATGAAC 
CGTGGAGGCT ATATGCCCAC CCCAACAGGG GCGAAGGTGG ACTATATTCA GGGGACACCG 
GTGAGTGTTC ATCTGCAGCC CTCCCTCTCC AGACAGAGCA GCTATACCAG TAATGGCACC 
CTCCCCAGGA CGGGACTAAA GAGGACACCA TCCTTAAAAC CTGATGTGCC ACCAAAGCCT 
TCCTTTGTTC CGCAAACCAC ATCTGTCAGA CCACTGAACA AGTACACGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_017317014.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172503.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu80174
Clone ID Related Accession (Same CDS sequence)	XM_017317014.1 , XM_017317014.2 , XM_030249365.1 , XM_030249366.1
Accession Version	XM_017317014.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT 
GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA 
TCAGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC 
CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG 
TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC 
GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC 
CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC 
AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC 
CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG 
ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA 
GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT 
ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC 
TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA 
TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG 
AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT 
CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT 
AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG 
AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT 
TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA 
CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC 
CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC 
TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG 
AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA 
TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172503.1
CDS	832..3984
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_017317014.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317014.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT 
GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA 
TCAGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC 
CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG 
TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC 
GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC 
CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC 
AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC 
CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG 
ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA 
GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT 
ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC 
TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA 
TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG 
AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT 
CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT 
AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG 
AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT 
TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA 
CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC 
CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC 
TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG 
AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA 
TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_017317014.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172503.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OMu80174
Clone ID Related Accession (Same CDS sequence)	XM_017317014.1 , XM_017317014.2 , XM_030249365.1 , XM_030249366.1
Accession Version	XM_017317014.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317014.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT 
GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA 
TCAGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC 
CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG 
TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC 
GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC 
CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC 
AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC 
CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG 
ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA 
GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT 
ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC 
TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA 
TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG 
AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT 
CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT 
AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG 
AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT 
TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA 
CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC 
CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC 
TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG 
AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA 
TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172503.1
CDS	830..3982
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_017317014.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317014.2

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT 
GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA 
TCAGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC 
CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG 
TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC 
GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC 
CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC 
AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC 
CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG 
ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA 
GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT 
ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC 
TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA 
TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG 
AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT 
CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT 
AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG 
AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT 
TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA 
CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC 
CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC 
TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG 
AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA 
TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_030249365.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105225.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OMu80174
Clone ID Related Accession (Same CDS sequence)	XM_017317014.1 , XM_017317014.2 , XM_030249365.1 , XM_030249366.1
Accession Version	XM_030249365.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT 
GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA 
TCAGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC 
CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG 
TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC 
GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC 
CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC 
AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC 
CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG 
ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA 
GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT 
ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC 
TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA 
TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG 
AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT 
CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT 
AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG 
AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT 
TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA 
CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC 
CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC 
TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG 
AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA 
TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105225.1
CDS	605..3757
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_030249365.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249365.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT 
GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA 
TCAGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC 
CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG 
TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC 
GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC 
CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC 
AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC 
CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG 
ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA 
GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT 
ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC 
TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA 
TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG 
AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT 
CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT 
AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG 
AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT 
TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA 
CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC 
CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC 
TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG 
AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA 
TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_030249366.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105226.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OMu80174
Clone ID Related Accession (Same CDS sequence)	XM_017317014.1 , XM_017317014.2 , XM_030249365.1 , XM_030249366.1
Accession Version	XM_030249366.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT 
GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA 
TCAGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC 
CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG 
TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC 
GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC 
CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC 
AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC 
CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG 
ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA 
GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT 
ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC 
TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA 
TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG 
AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT 
CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT 
AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG 
AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT 
TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA 
CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC 
CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC 
TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG 
AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA 
TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105226.1
CDS	8748..11900
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_030249366.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249366.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT 
GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA 
TCAGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC 
CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG 
TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC 
GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC 
CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC 
AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC 
CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG 
ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA 
GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT 
ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC 
TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA 
TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG 
AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT 
CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT 
AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG 
AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT 
TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA 
CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC 
CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC 
TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG 
AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA 
TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_030249358.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105218.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OMu87775
Clone ID Related Accession (Same CDS sequence)	XM_030249358.1
Accession Version	XM_030249358.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGACA TGGAGGTATC CTCATCTTCT GTTACCACTG TGGCAAGTAG CCCAGAAATT 
ACATCTAAAG TGATTGATAC CTGGAGACCT AAACTGACGA GCTCCCGGAA ATTTGTAGTT 
CAAGATGACC CAAATACTTC TGATTTTACT GATACTATAT CAGGTGTACG GTGGGAAGTC 
CAGTCTGGAG AATCCAATCA GATGGTCCAC ATGAATGTCC TCATCACCTG CGTGTTTGCC 
GCTTTTGTCT TGGGCGCGTT CATCGCAGGA GTGGCCGTGT ACTGCTACCG TGACATGTTC 
GTTCGGAAGA ACAGAAAGAT CCATAAAGAC GCAGAATCCG CCCAGTCGTG CACAGACTCC 
AGCGGAAGCT TCGCCAAGCT GAACGGCCTC TTTGACAGCC CCGTCAAGGA ATACCAGCAG 
AACATTGATT CTCCCAAACT CTACAGCAAC CTGCTGACCA GTCGGAAGGA ACTGCCACCA 
AACACGGATA CAAAGTCCAT GGCCGTGGAC CACAGAGGCC AGCCTCCCGA GCTGGCTGCT 
CTCCCCACGC CGGAATCCAC ACCTGTCCTC CACCAGAAGA CCCTGCAGGC CATGAAGAGC 
CACTCTGAGA AGGCCCACAG CCACGGTGCT TCAAGGAAAG AACACCCCCA GTTTTTTCCT 
TCTAGTCCTC CACCCCATTC CCCATTGAGT CACGGGCATA TCCCCAGTGC CATCGTTCTT 
CCAAACGCCA CTCACGACTA CAATACATCC TTCTCCAACT CGAATGCCCA CAAAGCCGAA 
AAGAAGCTTC AGAGCATGGA TCACCCTCTT ACGAAGTCAT CCAGTAAGCG GGAGCACCGG 
CGGTCTGTGG ATTCCAGGAA TACTCTCAAT GATCTCCTGA AGCATCTAAA TGACCCAAAC 
AGTAACCCCA AAGCCATCCT GGGAGAGATC CATATGGCTC ATCAAACCCT CATGCTGGAC 
CCGGTGGGAC CAATGGCTGA GGTCCCACCC AAGGTCCCTA ACCGGGAGGC ATCTCTATAC 
TCCCCTCCCT CCACACTCCC CAGAAATAGT CCAACCAAGA GAGTAGATGT CCCCACCACT 
CCTGGGGTGC CAATGACTTC TCTGGAAAGA CAAAGGGGTT ATCACAAAAA TTCCTCCCAG 
AGGCACTCTA TATCTGCCGT GCCTAAAAAC TTAAACTCAC CAAATGGTGT TTTGTTATCT 
AGACAGCCGA GTATGAACCG TGGAGGCTAT ATGCCCACCC CAACAGGGGC GAAGGTGGAC 
TATATTCAGG GGACACCGGT GAGTGTTCAT CTGCAGCCCT CCCTCTCCAG ACAGAGCAGC 
TATACCAGTA ATGGCACCCT CCCCAGGACG GGACTAAAGA GGACACCATC CTTAAAACCT 
GATGTGCCAC CAAAGCCTTC CTTTGTTCCG CAAACCACAT CTGTCAGACC ACTGAACAAG 
TACACGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105218.1
CDS	830..4021
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_030249358.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249358.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGACA TGGAGGTATC CTCATCTTCT GTTACCACTG TGGCAAGTAG CCCAGAAATT 
ACATCTAAAG TGATTGATAC CTGGAGACCT AAACTGACGA GCTCCCGGAA ATTTGTAGTT 
CAAGATGACC CAAATACTTC TGATTTTACT GATACTATAT CAGGTGTACG GTGGGAAGTC 
CAGTCTGGAG AATCCAATCA GATGGTCCAC ATGAATGTCC TCATCACCTG CGTGTTTGCC 
GCTTTTGTCT TGGGCGCGTT CATCGCAGGA GTGGCCGTGT ACTGCTACCG TGACATGTTC 
GTTCGGAAGA ACAGAAAGAT CCATAAAGAC GCAGAATCCG CCCAGTCGTG CACAGACTCC 
AGCGGAAGCT TCGCCAAGCT GAACGGCCTC TTTGACAGCC CCGTCAAGGA ATACCAGCAG 
AACATTGATT CTCCCAAACT CTACAGCAAC CTGCTGACCA GTCGGAAGGA ACTGCCACCA 
AACACGGATA CAAAGTCCAT GGCCGTGGAC CACAGAGGCC AGCCTCCCGA GCTGGCTGCT 
CTCCCCACGC CGGAATCCAC ACCTGTCCTC CACCAGAAGA CCCTGCAGGC CATGAAGAGC 
CACTCTGAGA AGGCCCACAG CCACGGTGCT TCAAGGAAAG AACACCCCCA GTTTTTTCCT 
TCTAGTCCTC CACCCCATTC CCCATTGAGT CACGGGCATA TCCCCAGTGC CATCGTTCTT 
CCAAACGCCA CTCACGACTA CAATACATCC TTCTCCAACT CGAATGCCCA CAAAGCCGAA 
AAGAAGCTTC AGAGCATGGA TCACCCTCTT ACGAAGTCAT CCAGTAAGCG GGAGCACCGG 
CGGTCTGTGG ATTCCAGGAA TACTCTCAAT GATCTCCTGA AGCATCTAAA TGACCCAAAC 
AGTAACCCCA AAGCCATCCT GGGAGAGATC CATATGGCTC ATCAAACCCT CATGCTGGAC 
CCGGTGGGAC CAATGGCTGA GGTCCCACCC AAGGTCCCTA ACCGGGAGGC ATCTCTATAC 
TCCCCTCCCT CCACACTCCC CAGAAATAGT CCAACCAAGA GAGTAGATGT CCCCACCACT 
CCTGGGGTGC CAATGACTTC TCTGGAAAGA CAAAGGGGTT ATCACAAAAA TTCCTCCCAG 
AGGCACTCTA TATCTGCCGT GCCTAAAAAC TTAAACTCAC CAAATGGTGT TTTGTTATCT 
AGACAGCCGA GTATGAACCG TGGAGGCTAT ATGCCCACCC CAACAGGGGC GAAGGTGGAC 
TATATTCAGG GGACACCGGT GAGTGTTCAT CTGCAGCCCT CCCTCTCCAG ACAGAGCAGC 
TATACCAGTA ATGGCACCCT CCCCAGGACG GGACTAAAGA GGACACCATC CTTAAAACCT 
GATGTGCCAC CAAAGCCTTC CTTTGTTCCG CAAACCACAT CTGTCAGACC ACTGAACAAG 
TACACGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( NM_199239.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_954709.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 5, mRNA.

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CloneID	OMu20434
Clone ID Related Accession (Same CDS sequence)	XM_017317019.2 , XM_017317019.1 , NM_199239.3
Accession Version	NM_199239.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-10
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform Sema6D-5 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC060680.1, AB091534.1 and AL935323.6. On Mar 21, 2014 this sequence version replaced NM_199239.2. Transcript Variant: This variant (5) contains an additional in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (Sema6D-5) that is longer than isoform Sema6D-1. The 5' UTR is incomplete because the evidence for this variant is for the CDS only, and multiple possible 5' UTR exon combinations exist. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB091534.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC 
CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG 
TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC 
GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC 
CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC 
AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC 
CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG 
ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA 
GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT 
ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC 
TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA 
TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG 
AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT 
CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT 
AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG 
AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT 
TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA 
CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC 
CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC 
TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG 
AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA 
TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954709.1
CDS	55..3147
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	NM_199239.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199239.3

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC 
CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG 
TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC 
GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC 
CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC 
AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC 
CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG 
ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA 
GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT 
ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC 
TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA 
TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG 
AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT 
CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT 
AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG 
AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT 
TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA 
CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC 
CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC 
TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG 
AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA 
TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( NM_172537.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766125.2 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 1, mRNA.

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CloneID	OMu20449
Clone ID Related Accession (Same CDS sequence)	XM_030249369.1 , XM_030249371.1 , NM_172537.4
Accession Version	NM_172537.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-10
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform Sema6D-1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB091532.1, BC060680.1 and AL935323.6. On Mar 21, 2014 this sequence version replaced NM_172537.3. Transcript Variant: This variant (1) encodes isoform Sema6D-1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB091532.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGGTG TACGGTGGGA AGTCCAGTCT GGAGAATCCA ATCAGATGGT CCACATGAAT 
GTCCTCATCA CCTGCGTGTT TGCCGCTTTT GTCTTGGGCG CGTTCATCGC AGGAGTGGCC 
GTGTACTGCT ACCGTGACAT GTTCGTTCGG AAGAACAGAA AGATCCATAA AGACGCAGAA 
TCCGCCCAGT CGTGCACAGA CTCCAGCGGA AGCTTCGCCA AGCTGAACGG CCTCTTTGAC 
AGCCCCGTCA AGGAATACCA GCAGAACATT GATTCTCCCA AACTCTACAG CAACCTGCTG 
ACCAGTCGGA AGGAACTGCC ACCAAACACG GATACAAAGT CCATGGCCGT GGACCACAGA 
GGCCAGCCTC CCGAGCTGGC TGCTCTCCCC ACGCCGGAAT CCACACCTGT CCTCCACCAG 
AAGACCCTGC AGGCCATGAA GAGCCACTCT GAGAAGGCCC ACAGCCACGG TGCTTCAAGG 
AAAGAACACC CCCAGTTTTT TCCTTCTAGT CCTCCACCCC ATTCCCCATT GAGTCACGGG 
CATATCCCCA GTGCCATCGT TCTTCCAAAC GCCACTCACG ACTACAATAC ATCCTTCTCC 
AACTCGAATG CCCACAAAGC CGAAAAGAAG CTTCAGAGCA TGGATCACCC TCTTACGAAG 
TCATCCAGTA AGCGGGAGCA CCGGCGGTCT GTGGATTCCA GGAATACTCT CAATGATCTC 
CTGAAGCATC TAAATGACCC AAACAGTAAC CCCAAAGCCA TCCTGGGAGA GATCCATATG 
GCTCATCAAA CCCTCATGCT GGACCCGGTG GGACCAATGG CTGAGGTCCC ACCCAAGGTC 
CCTAACCGGG AGGCATCTCT ATACTCCCCT CCCTCCACAC TCCCCAGAAA TAGTCCAACC 
AAGAGAGTAG ATGTCCCCAC CACTCCTGGG GTGCCAATGA CTTCTCTGGA AAGACAAAGG 
GGTTATCACA AAAATTCCTC CCAGAGGCAC TCTATATCTG CCGTGCCTAA AAACTTAAAC 
TCACCAAATG GTGTTTTGTT ATCTAGACAG CCGAGTATGA ACCGTGGAGG CTATATGCCC 
ACCCCAACAG GGGCGAAGGT GGACTATATT CAGGGGACAC CGGTGAGTGT TCATCTGCAG 
CCCTCCCTCT CCAGACAGAG CAGCTATACC AGTAATGGCA CCCTCCCCAG GACGGGACTA 
AAGAGGACAC CATCCTTAAA ACCTGATGTG CCACCAAAGC CTTCCTTTGT TCCGCAAACC 
ACATCTGTCA GACCACTGAA CAAGTACACG TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766125.2
CDS	820..3855
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	NM_172537.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172537.4

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGGTG TACGGTGGGA AGTCCAGTCT GGAGAATCCA ATCAGATGGT CCACATGAAT 
GTCCTCATCA CCTGCGTGTT TGCCGCTTTT GTCTTGGGCG CGTTCATCGC AGGAGTGGCC 
GTGTACTGCT ACCGTGACAT GTTCGTTCGG AAGAACAGAA AGATCCATAA AGACGCAGAA 
TCCGCCCAGT CGTGCACAGA CTCCAGCGGA AGCTTCGCCA AGCTGAACGG CCTCTTTGAC 
AGCCCCGTCA AGGAATACCA GCAGAACATT GATTCTCCCA AACTCTACAG CAACCTGCTG 
ACCAGTCGGA AGGAACTGCC ACCAAACACG GATACAAAGT CCATGGCCGT GGACCACAGA 
GGCCAGCCTC CCGAGCTGGC TGCTCTCCCC ACGCCGGAAT CCACACCTGT CCTCCACCAG 
AAGACCCTGC AGGCCATGAA GAGCCACTCT GAGAAGGCCC ACAGCCACGG TGCTTCAAGG 
AAAGAACACC CCCAGTTTTT TCCTTCTAGT CCTCCACCCC ATTCCCCATT GAGTCACGGG 
CATATCCCCA GTGCCATCGT TCTTCCAAAC GCCACTCACG ACTACAATAC ATCCTTCTCC 
AACTCGAATG CCCACAAAGC CGAAAAGAAG CTTCAGAGCA TGGATCACCC TCTTACGAAG 
TCATCCAGTA AGCGGGAGCA CCGGCGGTCT GTGGATTCCA GGAATACTCT CAATGATCTC 
CTGAAGCATC TAAATGACCC AAACAGTAAC CCCAAAGCCA TCCTGGGAGA GATCCATATG 
GCTCATCAAA CCCTCATGCT GGACCCGGTG GGACCAATGG CTGAGGTCCC ACCCAAGGTC 
CCTAACCGGG AGGCATCTCT ATACTCCCCT CCCTCCACAC TCCCCAGAAA TAGTCCAACC 
AAGAGAGTAG ATGTCCCCAC CACTCCTGGG GTGCCAATGA CTTCTCTGGA AAGACAAAGG 
GGTTATCACA AAAATTCCTC CCAGAGGCAC TCTATATCTG CCGTGCCTAA AAACTTAAAC 
TCACCAAATG GTGTTTTGTT ATCTAGACAG CCGAGTATGA ACCGTGGAGG CTATATGCCC 
ACCCCAACAG GGGCGAAGGT GGACTATATT CAGGGGACAC CGGTGAGTGT TCATCTGCAG 
CCCTCCCTCT CCAGACAGAG CAGCTATACC AGTAATGGCA CCCTCCCCAG GACGGGACTA 
AAGAGGACAC CATCCTTAAA ACCTGATGTG CCACCAAAGC CTTCCTTTGT TCCGCAAACC 
ACATCTGTCA GACCACTGAA CAAGTACACG TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( NM_199241.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_954711.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 4, mRNA.

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CloneID	OMu20490
Clone ID Related Accession (Same CDS sequence)	XM_030249343.1 , XM_030249345.1 , XM_017317001.1 , XM_017317001.2 , NM_199241.3
Accession Version	NM_199241.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-10
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform Sema6D-4 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC060680.1, AB091536.1 and AL935323.6. On Mar 21, 2014 this sequence version replaced NM_199241.2. Transcript Variant: This variant (4) uses an alternate in-frame splice site and contains two additional in-frame exons in the central coding region, compared to variant 1. The encoded isoform (Sema6D-4) is longer than isoform Sema6D-1. The 5' UTR is incomplete because the evidence for this variant is for the CDS only, and multiple possible 5' UTR exon combinations exist. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB091536.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG 
GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC 
TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA 
GGTATCCCAA AGGGTGTACG GTGGGAAGTC CAGTCTGGAG AATCCAATCA GATGGTCCAC 
ATGAATGTCC TCATCACCTG CGTGTTTGCC GCTTTTGTCT TGGGCGCGTT CATCGCAGGA 
GTGGCCGTGT ACTGCTACCG TGACATGTTC GTTCGGAAGA ACAGAAAGAT CCATAAAGAC 
GCAGAATCCG CCCAGTCGTG CACAGACTCC AGCGGAAGCT TCGCCAAGCT GAACGGCCTC 
TTTGACAGCC CCGTCAAGGA ATACCAGCAG AACATTGATT CTCCCAAACT CTACAGCAAC 
CTGCTGACCA GTCGGAAGGA ACTGCCACCA AACACGGATA CAAAGTCCAT GGCCGTGGAC 
CACAGAGGCC AGCCTCCCGA GCTGGCTGCT CTCCCCACGC CGGAATCCAC ACCTGTCCTC 
CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCTGAGA AGGCCCACAG CCACGGTGCT 
TCAAGGAAAG AACACCCCCA GTTTTTTCCT TCTAGTCCTC CACCCCATTC CCCATTGAGT 
CACGGGCATA TCCCCAGTGC CATCGTTCTT CCAAACGCCA CTCACGACTA CAATACATCC 
TTCTCCAACT CGAATGCCCA CAAAGCCGAA AAGAAGCTTC AGAGCATGGA TCACCCTCTT 
ACGAAGTCAT CCAGTAAGCG GGAGCACCGG CGGTCTGTGG ATTCCAGGAA TACTCTCAAT 
GATCTCCTGA AGCATCTAAA TGACCCAAAC AGTAACCCCA AAGCCATCCT GGGAGAGATC 
CATATGGCTC ATCAAACCCT CATGCTGGAC CCGGTGGGAC CAATGGCTGA GGTCCCACCC 
AAGGTCCCTA ACCGGGAGGC ATCTCTATAC TCCCCTCCCT CCACACTCCC CAGAAATAGT 
CCAACCAAGA GAGTAGATGT CCCCACCACT CCTGGGGTGC CAATGACTTC TCTGGAAAGA 
CAAAGGGGTT ATCACAAAAA TTCCTCCCAG AGGCACTCTA TATCTGCCGT GCCTAAAAAC 
TTAAACTCAC CAAATGGTGT TTTGTTATCT AGACAGCCGA GTATGAACCG TGGAGGCTAT 
ATGCCCACCC CAACAGGGGC GAAGGTGGAC TATATTCAGG GGACACCGGT GAGTGTTCAT 
CTGCAGCCCT CCCTCTCCAG ACAGAGCAGC TATACCAGTA ATGGCACCCT CCCCAGGACG 
GGACTAAAGA GGACACCATC CTTAAAACCT GATGTGCCAC CAAAGCCTTC CTTTGTTCCG 
CAAACCACAT CTGTCAGACC ACTGAACAAG TACACGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954711.1
CDS	55..3276
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	NM_199241.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199241.3

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG 
GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC 
TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA 
GGTATCCCAA AGGGTGTACG GTGGGAAGTC CAGTCTGGAG AATCCAATCA GATGGTCCAC 
ATGAATGTCC TCATCACCTG CGTGTTTGCC GCTTTTGTCT TGGGCGCGTT CATCGCAGGA 
GTGGCCGTGT ACTGCTACCG TGACATGTTC GTTCGGAAGA ACAGAAAGAT CCATAAAGAC 
GCAGAATCCG CCCAGTCGTG CACAGACTCC AGCGGAAGCT TCGCCAAGCT GAACGGCCTC 
TTTGACAGCC CCGTCAAGGA ATACCAGCAG AACATTGATT CTCCCAAACT CTACAGCAAC 
CTGCTGACCA GTCGGAAGGA ACTGCCACCA AACACGGATA CAAAGTCCAT GGCCGTGGAC 
CACAGAGGCC AGCCTCCCGA GCTGGCTGCT CTCCCCACGC CGGAATCCAC ACCTGTCCTC 
CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCTGAGA AGGCCCACAG CCACGGTGCT 
TCAAGGAAAG AACACCCCCA GTTTTTTCCT TCTAGTCCTC CACCCCATTC CCCATTGAGT 
CACGGGCATA TCCCCAGTGC CATCGTTCTT CCAAACGCCA CTCACGACTA CAATACATCC 
TTCTCCAACT CGAATGCCCA CAAAGCCGAA AAGAAGCTTC AGAGCATGGA TCACCCTCTT 
ACGAAGTCAT CCAGTAAGCG GGAGCACCGG CGGTCTGTGG ATTCCAGGAA TACTCTCAAT 
GATCTCCTGA AGCATCTAAA TGACCCAAAC AGTAACCCCA AAGCCATCCT GGGAGAGATC 
CATATGGCTC ATCAAACCCT CATGCTGGAC CCGGTGGGAC CAATGGCTGA GGTCCCACCC 
AAGGTCCCTA ACCGGGAGGC ATCTCTATAC TCCCCTCCCT CCACACTCCC CAGAAATAGT 
CCAACCAAGA GAGTAGATGT CCCCACCACT CCTGGGGTGC CAATGACTTC TCTGGAAAGA 
CAAAGGGGTT ATCACAAAAA TTCCTCCCAG AGGCACTCTA TATCTGCCGT GCCTAAAAAC 
TTAAACTCAC CAAATGGTGT TTTGTTATCT AGACAGCCGA GTATGAACCG TGGAGGCTAT 
ATGCCCACCC CAACAGGGGC GAAGGTGGAC TATATTCAGG GGACACCGGT GAGTGTTCAT 
CTGCAGCCCT CCCTCTCCAG ACAGAGCAGC TATACCAGTA ATGGCACCCT CCCCAGGACG 
GGACTAAAGA GGACACCATC CTTAAAACCT GATGTGCCAC CAAAGCCTTC CTTTGTTCCG 
CAAACCACAT CTGTCAGACC ACTGAACAAG TACACGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( NM_199240.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_954710.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 6, mRNA.

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CloneID	OMu20528
Clone ID Related Accession (Same CDS sequence)	XM_017317012.2 , XM_030249363.1 , XM_011239427.3 , XM_030249361.1 , XM_017317012.1 , XM_011239427.2 , NM_199240.3
Accession Version	NM_199240.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-10
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform Sema6D-6 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC060680.1, AB091535.1 and AL935323.6. On Mar 21, 2014 this sequence version replaced NM_199240.2. Transcript Variant: This variant (6) uses an alternate in-frame splice site and contains an additional in-frame exon in the central coding region, compared to variant 1. The encoded isoform (Sema6D-6) is longer than isoform Sema6D-1. The 5' UTR is incomplete because the evidence for this variant is for the CDS only, and multiple possible 5' UTR exon combinations exist. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB091535.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT 
GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA 
TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC 
CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA 
GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA 
GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC 
CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC 
AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG 
GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC 
CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT 
GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG 
AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA 
TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT 
CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC 
AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG 
ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA 
CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT 
AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA 
AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA 
AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC 
TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT 
CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG 
ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT 
CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954710.1
CDS	55..3219
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	NM_199240.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199240.3

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT 
GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA 
TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC 
CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA 
GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA 
GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC 
CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC 
AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG 
GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC 
CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT 
GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG 
AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA 
TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT 
CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC 
AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG 
ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA 
CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT 
AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA 
AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA 
AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC 
TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT 
CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG 
ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT 
CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( NM_001291000.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277929.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OMu20556
Clone ID Related Accession (Same CDS sequence)	NM_001291000.1 , XM_030249347.1 , XM_030249351.1
Accession Version	NM_001291000.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-10
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB091532.1, AK031307.1, AL935323.6 and BC060680.1. On Mar 21, 2014 this sequence version replaced XM_006499117.1. Transcript Variant: This variant (7) uses an alternate in-frame splice site and contains two additional in-frame exons in the central coding region, compared to variant 1. The encoded isoform (3) is longer than isoform Sema6D-1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK031307.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG 
GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC 
TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA 
GGTGTACGGT GGGAAGTCCA GTCTGGAGAA TCCAATCAGA TGGTCCACAT GAATGTCCTC 
ATCACCTGCG TGTTTGCCGC TTTTGTCTTG GGCGCGTTCA TCGCAGGAGT GGCCGTGTAC 
TGCTACCGTG ACATGTTCGT TCGGAAGAAC AGAAAGATCC ATAAAGACGC AGAATCCGCC 
CAGTCGTGCA CAGACTCCAG CGGAAGCTTC GCCAAGCTGA ACGGCCTCTT TGACAGCCCC 
GTCAAGGAAT ACCAGCAGAA CATTGATTCT CCCAAACTCT ACAGCAACCT GCTGACCAGT 
CGGAAGGAAC TGCCACCAAA CACGGATACA AAGTCCATGG CCGTGGACCA CAGAGGCCAG 
CCTCCCGAGC TGGCTGCTCT CCCCACGCCG GAATCCACAC CTGTCCTCCA CCAGAAGACC 
CTGCAGGCCA TGAAGAGCCA CTCTGAGAAG GCCCACAGCC ACGGTGCTTC AAGGAAAGAA 
CACCCCCAGT TTTTTCCTTC TAGTCCTCCA CCCCATTCCC CATTGAGTCA CGGGCATATC 
CCCAGTGCCA TCGTTCTTCC AAACGCCACT CACGACTACA ATACATCCTT CTCCAACTCG 
AATGCCCACA AAGCCGAAAA GAAGCTTCAG AGCATGGATC ACCCTCTTAC GAAGTCATCC 
AGTAAGCGGG AGCACCGGCG GTCTGTGGAT TCCAGGAATA CTCTCAATGA TCTCCTGAAG 
CATCTAAATG ACCCAAACAG TAACCCCAAA GCCATCCTGG GAGAGATCCA TATGGCTCAT 
CAAACCCTCA TGCTGGACCC GGTGGGACCA ATGGCTGAGG TCCCACCCAA GGTCCCTAAC 
CGGGAGGCAT CTCTATACTC CCCTCCCTCC ACACTCCCCA GAAATAGTCC AACCAAGAGA 
GTAGATGTCC CCACCACTCC TGGGGTGCCA ATGACTTCTC TGGAAAGACA AAGGGGTTAT 
CACAAAAATT CCTCCCAGAG GCACTCTATA TCTGCCGTGC CTAAAAACTT AAACTCACCA 
AATGGTGTTT TGTTATCTAG ACAGCCGAGT ATGAACCGTG GAGGCTATAT GCCCACCCCA 
ACAGGGGCGA AGGTGGACTA TATTCAGGGG ACACCGGTGA GTGTTCATCT GCAGCCCTCC 
CTCTCCAGAC AGAGCAGCTA TACCAGTAAT GGCACCCTCC CCAGGACGGG ACTAAAGAGG 
ACACCATCCT TAAAACCTGA TGTGCCACCA AAGCCTTCCT TTGTTCCGCA AACCACATCT 
GTCAGACCAC TGAACAAGTA CACGTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277929.1
CDS	820..4029
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	NM_001291000.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291000.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG 
GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC 
TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA 
GGTGTACGGT GGGAAGTCCA GTCTGGAGAA TCCAATCAGA TGGTCCACAT GAATGTCCTC 
ATCACCTGCG TGTTTGCCGC TTTTGTCTTG GGCGCGTTCA TCGCAGGAGT GGCCGTGTAC 
TGCTACCGTG ACATGTTCGT TCGGAAGAAC AGAAAGATCC ATAAAGACGC AGAATCCGCC 
CAGTCGTGCA CAGACTCCAG CGGAAGCTTC GCCAAGCTGA ACGGCCTCTT TGACAGCCCC 
GTCAAGGAAT ACCAGCAGAA CATTGATTCT CCCAAACTCT ACAGCAACCT GCTGACCAGT 
CGGAAGGAAC TGCCACCAAA CACGGATACA AAGTCCATGG CCGTGGACCA CAGAGGCCAG 
CCTCCCGAGC TGGCTGCTCT CCCCACGCCG GAATCCACAC CTGTCCTCCA CCAGAAGACC 
CTGCAGGCCA TGAAGAGCCA CTCTGAGAAG GCCCACAGCC ACGGTGCTTC AAGGAAAGAA 
CACCCCCAGT TTTTTCCTTC TAGTCCTCCA CCCCATTCCC CATTGAGTCA CGGGCATATC 
CCCAGTGCCA TCGTTCTTCC AAACGCCACT CACGACTACA ATACATCCTT CTCCAACTCG 
AATGCCCACA AAGCCGAAAA GAAGCTTCAG AGCATGGATC ACCCTCTTAC GAAGTCATCC 
AGTAAGCGGG AGCACCGGCG GTCTGTGGAT TCCAGGAATA CTCTCAATGA TCTCCTGAAG 
CATCTAAATG ACCCAAACAG TAACCCCAAA GCCATCCTGG GAGAGATCCA TATGGCTCAT 
CAAACCCTCA TGCTGGACCC GGTGGGACCA ATGGCTGAGG TCCCACCCAA GGTCCCTAAC 
CGGGAGGCAT CTCTATACTC CCCTCCCTCC ACACTCCCCA GAAATAGTCC AACCAAGAGA 
GTAGATGTCC CCACCACTCC TGGGGTGCCA ATGACTTCTC TGGAAAGACA AAGGGGTTAT 
CACAAAAATT CCTCCCAGAG GCACTCTATA TCTGCCGTGC CTAAAAACTT AAACTCACCA 
AATGGTGTTT TGTTATCTAG ACAGCCGAGT ATGAACCGTG GAGGCTATAT GCCCACCCCA 
ACAGGGGCGA AGGTGGACTA TATTCAGGGG ACACCGGTGA GTGTTCATCT GCAGCCCTCC 
CTCTCCAGAC AGAGCAGCTA TACCAGTAAT GGCACCCTCC CCAGGACGGG ACTAAAGAGG 
ACACCATCCT TAAAACCTGA TGTGCCACCA AAGCCTTCCT TTGTTCCGCA AACCACATCT 
GTCAGACCAC TGAACAAGTA CACGTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_017317019.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172508.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMu20434
Clone ID Related Accession (Same CDS sequence)	XM_017317019.2 , XM_017317019.1 , NM_199239.3
Accession Version	XM_017317019.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC 
CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG 
TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC 
GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC 
CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC 
AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC 
CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG 
ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA 
GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT 
ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC 
TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA 
TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG 
AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT 
CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT 
AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG 
AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT 
TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA 
CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC 
CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC 
TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG 
AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA 
TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172508.1
CDS	832..3924
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_017317019.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317019.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGGTGTAC GGTGGGAAGT CCAGTCTGGA GAATCCAATC AGATGGTCCA CATGAATGTC 
CTCATCACCT GCGTGTTTGC CGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCCGTG 
TACTGCTACC GTGACATGTT CGTTCGGAAG AACAGAAAGA TCCATAAAGA CGCAGAATCC 
GCCCAGTCGT GCACAGACTC CAGCGGAAGC TTCGCCAAGC TGAACGGCCT CTTTGACAGC 
CCCGTCAAGG AATACCAGCA GAACATTGAT TCTCCCAAAC TCTACAGCAA CCTGCTGACC 
AGTCGGAAGG AACTGCCACC AAACACGGAT ACAAAGTCCA TGGCCGTGGA CCACAGAGGC 
CAGCCTCCCG AGCTGGCTGC TCTCCCCACG CCGGAATCCA CACCTGTCCT CCACCAGAAG 
ACCCTGCAGG CCATGAAGAG CCACTCTGAG AAGGCCCACA GCCACGGTGC TTCAAGGAAA 
GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCATTGAG TCACGGGCAT 
ATCCCCAGTG CCATCGTTCT TCCAAACGCC ACTCACGACT ACAATACATC CTTCTCCAAC 
TCGAATGCCC ACAAAGCCGA AAAGAAGCTT CAGAGCATGG ATCACCCTCT TACGAAGTCA 
TCCAGTAAGC GGGAGCACCG GCGGTCTGTG GATTCCAGGA ATACTCTCAA TGATCTCCTG 
AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCC TGGGAGAGAT CCATATGGCT 
CATCAAACCC TCATGCTGGA CCCGGTGGGA CCAATGGCTG AGGTCCCACC CAAGGTCCCT 
AACCGGGAGG CATCTCTATA CTCCCCTCCC TCCACACTCC CCAGAAATAG TCCAACCAAG 
AGAGTAGATG TCCCCACCAC TCCTGGGGTG CCAATGACTT CTCTGGAAAG ACAAAGGGGT 
TATCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA 
CCAAATGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGCTA TATGCCCACC 
CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTTCA TCTGCAGCCC 
TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TCCCCAGGAC GGGACTAAAG 
AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC GCAAACCACA 
TCTGTCAGAC CACTGAACAA GTACACGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_017317001.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172490.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu20490
Clone ID Related Accession (Same CDS sequence)	XM_030249343.1 , XM_030249345.1 , XM_017317001.1 , XM_017317001.2 , NM_199241.3
Accession Version	XM_017317001.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG 
GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC 
TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA 
GGTATCCCAA AGGGTGTACG GTGGGAAGTC CAGTCTGGAG AATCCAATCA GATGGTCCAC 
ATGAATGTCC TCATCACCTG CGTGTTTGCC GCTTTTGTCT TGGGCGCGTT CATCGCAGGA 
GTGGCCGTGT ACTGCTACCG TGACATGTTC GTTCGGAAGA ACAGAAAGAT CCATAAAGAC 
GCAGAATCCG CCCAGTCGTG CACAGACTCC AGCGGAAGCT TCGCCAAGCT GAACGGCCTC 
TTTGACAGCC CCGTCAAGGA ATACCAGCAG AACATTGATT CTCCCAAACT CTACAGCAAC 
CTGCTGACCA GTCGGAAGGA ACTGCCACCA AACACGGATA CAAAGTCCAT GGCCGTGGAC 
CACAGAGGCC AGCCTCCCGA GCTGGCTGCT CTCCCCACGC CGGAATCCAC ACCTGTCCTC 
CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCTGAGA AGGCCCACAG CCACGGTGCT 
TCAAGGAAAG AACACCCCCA GTTTTTTCCT TCTAGTCCTC CACCCCATTC CCCATTGAGT 
CACGGGCATA TCCCCAGTGC CATCGTTCTT CCAAACGCCA CTCACGACTA CAATACATCC 
TTCTCCAACT CGAATGCCCA CAAAGCCGAA AAGAAGCTTC AGAGCATGGA TCACCCTCTT 
ACGAAGTCAT CCAGTAAGCG GGAGCACCGG CGGTCTGTGG ATTCCAGGAA TACTCTCAAT 
GATCTCCTGA AGCATCTAAA TGACCCAAAC AGTAACCCCA AAGCCATCCT GGGAGAGATC 
CATATGGCTC ATCAAACCCT CATGCTGGAC CCGGTGGGAC CAATGGCTGA GGTCCCACCC 
AAGGTCCCTA ACCGGGAGGC ATCTCTATAC TCCCCTCCCT CCACACTCCC CAGAAATAGT 
CCAACCAAGA GAGTAGATGT CCCCACCACT CCTGGGGTGC CAATGACTTC TCTGGAAAGA 
CAAAGGGGTT ATCACAAAAA TTCCTCCCAG AGGCACTCTA TATCTGCCGT GCCTAAAAAC 
TTAAACTCAC CAAATGGTGT TTTGTTATCT AGACAGCCGA GTATGAACCG TGGAGGCTAT 
ATGCCCACCC CAACAGGGGC GAAGGTGGAC TATATTCAGG GGACACCGGT GAGTGTTCAT 
CTGCAGCCCT CCCTCTCCAG ACAGAGCAGC TATACCAGTA ATGGCACCCT CCCCAGGACG 
GGACTAAAGA GGACACCATC CTTAAAACCT GATGTGCCAC CAAAGCCTTC CTTTGTTCCG 
CAAACCACAT CTGTCAGACC ACTGAACAAG TACACGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172490.1
CDS	832..4053
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_017317001.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317001.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCCT CATCTTCTGT TACCACTGTG 
GCAAGTAGCC CAGAAATTAC ATCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACGAGC 
TCCCGGAAAT TTGTAGTTCA AGATGACCCA AATACTTCTG ATTTTACTGA TACTATATCA 
GGTATCCCAA AGGGTGTACG GTGGGAAGTC CAGTCTGGAG AATCCAATCA GATGGTCCAC 
ATGAATGTCC TCATCACCTG CGTGTTTGCC GCTTTTGTCT TGGGCGCGTT CATCGCAGGA 
GTGGCCGTGT ACTGCTACCG TGACATGTTC GTTCGGAAGA ACAGAAAGAT CCATAAAGAC 
GCAGAATCCG CCCAGTCGTG CACAGACTCC AGCGGAAGCT TCGCCAAGCT GAACGGCCTC 
TTTGACAGCC CCGTCAAGGA ATACCAGCAG AACATTGATT CTCCCAAACT CTACAGCAAC 
CTGCTGACCA GTCGGAAGGA ACTGCCACCA AACACGGATA CAAAGTCCAT GGCCGTGGAC 
CACAGAGGCC AGCCTCCCGA GCTGGCTGCT CTCCCCACGC CGGAATCCAC ACCTGTCCTC 
CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCTGAGA AGGCCCACAG CCACGGTGCT 
TCAAGGAAAG AACACCCCCA GTTTTTTCCT TCTAGTCCTC CACCCCATTC CCCATTGAGT 
CACGGGCATA TCCCCAGTGC CATCGTTCTT CCAAACGCCA CTCACGACTA CAATACATCC 
TTCTCCAACT CGAATGCCCA CAAAGCCGAA AAGAAGCTTC AGAGCATGGA TCACCCTCTT 
ACGAAGTCAT CCAGTAAGCG GGAGCACCGG CGGTCTGTGG ATTCCAGGAA TACTCTCAAT 
GATCTCCTGA AGCATCTAAA TGACCCAAAC AGTAACCCCA AAGCCATCCT GGGAGAGATC 
CATATGGCTC ATCAAACCCT CATGCTGGAC CCGGTGGGAC CAATGGCTGA GGTCCCACCC 
AAGGTCCCTA ACCGGGAGGC ATCTCTATAC TCCCCTCCCT CCACACTCCC CAGAAATAGT 
CCAACCAAGA GAGTAGATGT CCCCACCACT CCTGGGGTGC CAATGACTTC TCTGGAAAGA 
CAAAGGGGTT ATCACAAAAA TTCCTCCCAG AGGCACTCTA TATCTGCCGT GCCTAAAAAC 
TTAAACTCAC CAAATGGTGT TTTGTTATCT AGACAGCCGA GTATGAACCG TGGAGGCTAT 
ATGCCCACCC CAACAGGGGC GAAGGTGGAC TATATTCAGG GGACACCGGT GAGTGTTCAT 
CTGCAGCCCT CCCTCTCCAG ACAGAGCAGC TATACCAGTA ATGGCACCCT CCCCAGGACG 
GGACTAAAGA GGACACCATC CTTAAAACCT GATGTGCCAC CAAAGCCTTC CTTTGTTCCG 
CAAACCACAT CTGTCAGACC ACTGAACAAG TACACGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_017317012.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172501.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X8, mRNA.

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CloneID	OMu20528
Clone ID Related Accession (Same CDS sequence)	XM_017317012.2 , XM_030249363.1 , XM_011239427.3 , XM_030249361.1 , XM_017317012.1 , XM_011239427.2 , NM_199240.3
Accession Version	XM_017317012.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT 
GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA 
TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC 
CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA 
GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA 
GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC 
CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC 
AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG 
GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC 
CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT 
GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG 
AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA 
TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT 
CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC 
AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG 
ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA 
CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT 
AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA 
AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA 
AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC 
TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT 
CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG 
ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT 
CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172501.1
CDS	832..3996
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_017317012.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317012.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT 
GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA 
TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC 
CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA 
GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA 
GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC 
CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC 
AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG 
GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC 
CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT 
GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG 
AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA 
TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT 
CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC 
AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG 
ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA 
CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT 
AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA 
AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA 
AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC 
TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT 
CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG 
ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT 
CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_011239427.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237729.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X9, mRNA.

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CloneID	OMu20528
Clone ID Related Accession (Same CDS sequence)	XM_017317012.2 , XM_030249363.1 , XM_011239427.3 , XM_030249361.1 , XM_017317012.1 , XM_011239427.2 , NM_199240.3
Accession Version	XM_011239427.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011239427.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT 
GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA 
TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC 
CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA 
GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA 
GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC 
CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC 
AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG 
GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC 
CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT 
GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG 
AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA 
TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT 
CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC 
AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG 
ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA 
CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT 
AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA 
AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA 
AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC 
TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT 
CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG 
ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT 
CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237729.1
CDS	630..3794
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_011239427.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239427.2

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAC ATGGAGGTAT CCTCATCTTC TGTTACCACT 
GTGGCAAGTA GCCCAGAAAT TACATCTAAA GTGATTGATA CCTGGAGACC TAAACTGACG 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAATACTT CTGATTTTAC TGATACTATA 
TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAATCCAA TCAGATGGTC 
CACATGAATG TCCTCATCAC CTGCGTGTTT GCCGCTTTTG TCTTGGGCGC GTTCATCGCA 
GGAGTGGCCG TGTACTGCTA CCGTGACATG TTCGTTCGGA AGAACAGAAA GATCCATAAA 
GACGCAGAAT CCGCCCAGTC GTGCACAGAC TCCAGCGGAA GCTTCGCCAA GCTGAACGGC 
CTCTTTGACA GCCCCGTCAA GGAATACCAG CAGAACATTG ATTCTCCCAA ACTCTACAGC 
AACCTGCTGA CCAGTCGGAA GGAACTGCCA CCAAACACGG ATACAAAGTC CATGGCCGTG 
GACCACAGAG GCCAGCCTCC CGAGCTGGCT GCTCTCCCCA CGCCGGAATC CACACCTGTC 
CTCCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCTG AGAAGGCCCA CAGCCACGGT 
GCTTCAAGGA AAGAACACCC CCAGTTTTTT CCTTCTAGTC CTCCACCCCA TTCCCCATTG 
AGTCACGGGC ATATCCCCAG TGCCATCGTT CTTCCAAACG CCACTCACGA CTACAATACA 
TCCTTCTCCA ACTCGAATGC CCACAAAGCC GAAAAGAAGC TTCAGAGCAT GGATCACCCT 
CTTACGAAGT CATCCAGTAA GCGGGAGCAC CGGCGGTCTG TGGATTCCAG GAATACTCTC 
AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CCTGGGAGAG 
ATCCATATGG CTCATCAAAC CCTCATGCTG GACCCGGTGG GACCAATGGC TGAGGTCCCA 
CCCAAGGTCC CTAACCGGGA GGCATCTCTA TACTCCCCTC CCTCCACACT CCCCAGAAAT 
AGTCCAACCA AGAGAGTAGA TGTCCCCACC ACTCCTGGGG TGCCAATGAC TTCTCTGGAA 
AGACAAAGGG GTTATCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC CGTGCCTAAA 
AACTTAAACT CACCAAATGG TGTTTTGTTA TCTAGACAGC CGAGTATGAA CCGTGGAGGC 
TATATGCCCA CCCCAACAGG GGCGAAGGTG GACTATATTC AGGGGACACC GGTGAGTGTT 
CATCTGCAGC CCTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTCCCCAGG 
ACGGGACTAA AGAGGACACC ATCCTTAAAA CCTGATGTGC CACCAAAGCC TTCCTTTGTT 
CCGCAAACCA CATCTGTCAG ACCACTGAAC AAGTACACGT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_006499111.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499174.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X2, mRNA.

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CloneID	OMu30226
Clone ID Related Accession (Same CDS sequence)	XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
Accession Version	XM_006499111.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	semaphorin-6D isoform X5
Comment

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006499174.1

Translation

MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_006499111.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499111.2

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_006499111.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499174.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu30226
Clone ID Related Accession (Same CDS sequence)	XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
Accession Version	XM_006499111.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499111.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499174.1
CDS	832..4092
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_006499111.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499111.3

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_006499114.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499177.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu30226
Clone ID Related Accession (Same CDS sequence)	XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
Accession Version	XM_006499114.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499114.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499177.1
CDS	859..4119
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_006499114.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499114.3

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_006499113.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499176.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu30226
Clone ID Related Accession (Same CDS sequence)	XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
Accession Version	XM_006499113.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	semaphorin-6D isoform X5
Comment

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006499176.1

Translation

MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_006499113.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499113.2

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_006499112.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499175.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu30226
Clone ID Related Accession (Same CDS sequence)	XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
Accession Version	XM_006499112.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	semaphorin-6D isoform X5
Comment

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006499175.1

Translation

MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_006499112.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499112.2

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_006499112.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499175.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X2, mRNA.

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CloneID	OMu30226
Clone ID Related Accession (Same CDS sequence)	XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
Accession Version	XM_006499112.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499112.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499175.1
CDS	369..3629
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_006499112.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499112.3

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_006499114.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499177.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu30226
Clone ID Related Accession (Same CDS sequence)	XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
Accession Version	XM_006499114.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	semaphorin-6D isoform X5
Comment

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006499177.1

Translation

MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_006499114.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499114.2

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_006499113.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499176.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu30226
Clone ID Related Accession (Same CDS sequence)	XM_006499111.2 , XM_006499111.3 , XM_006499114.3 , XM_030249335.1 , XM_006499113.2 , XM_006499112.2 , XM_006499112.3 , XM_006499111.4 , XM_006499112.4 , XM_030249334.1 , XM_006499113.4 , XM_006499114.4 , XM_006499114.2 , XM_006499113.3
Accession Version	XM_006499113.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499113.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499176.1
CDS	438..3698
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_006499113.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499113.3

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA ATCCAATCAG ATGGTCCACA TGAATGTCCT CATCACCTGC 
GTGTTTGCCG CTTTTGTCTT GGGCGCGTTC ATCGCAGGAG TGGCCGTGTA CTGCTACCGT 
GACATGTTCG TTCGGAAGAA CAGAAAGATC CATAAAGACG CAGAATCCGC CCAGTCGTGC 
ACAGACTCCA GCGGAAGCTT CGCCAAGCTG AACGGCCTCT TTGACAGCCC CGTCAAGGAA 
TACCAGCAGA ACATTGATTC TCCCAAACTC TACAGCAACC TGCTGACCAG TCGGAAGGAA 
CTGCCACCAA ACACGGATAC AAAGTCCATG GCCGTGGACC ACAGAGGCCA GCCTCCCGAG 
CTGGCTGCTC TCCCCACGCC GGAATCCACA CCTGTCCTCC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCTGAGAA GGCCCACAGC CACGGTGCTT CAAGGAAAGA ACACCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCCCATTCC CCATTGAGTC ACGGGCATAT CCCCAGTGCC 
ATCGTTCTTC CAAACGCCAC TCACGACTAC AATACATCCT TCTCCAACTC GAATGCCCAC 
AAAGCCGAAA AGAAGCTTCA GAGCATGGAT CACCCTCTTA CGAAGTCATC CAGTAAGCGG 
GAGCACCGGC GGTCTGTGGA TTCCAGGAAT ACTCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCCTG GGAGAGATCC ATATGGCTCA TCAAACCCTC 
ATGCTGGACC CGGTGGGACC AATGGCTGAG GTCCCACCCA AGGTCCCTAA CCGGGAGGCA 
TCTCTATACT CCCCTCCCTC CACACTCCCC AGAAATAGTC CAACCAAGAG AGTAGATGTC 
CCCACCACTC CTGGGGTGCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA TCACAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCTGCCGTG CCTAAAAACT TAAACTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCGAG TATGAACCGT GGAGGCTATA TGCCCACCCC AACAGGGGCG 
AAGGTGGACT ATATTCAGGG GACACCGGTG AGTGTTCATC TGCAGCCCTC CCTCTCCAGA 
CAGAGCAGCT ATACCAGTAA TGGCACCCTC CCCAGGACGG GACTAAAGAG GACACCATCC 
TTAAAACCTG ATGTGCCACC AAAGCCTTCC TTTGTTCCGC AAACCACATC TGTCAGACCA 
CTGAACAAGT ACACGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_017316995.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172484.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu72911
Clone ID Related Accession (Same CDS sequence)	XM_017316995.2 , XM_017316995.1
Accession Version	XM_017316995.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTGTACGGTG GGAAGTCCAG 
TCTGGAGAAT CCAATCAGAT GGTCCACATG AATGTCCTCA TCACCTGCGT GTTTGCCGCT 
TTTGTCTTGG GCGCGTTCAT CGCAGGAGTG GCCGTGTACT GCTACCGTGA CATGTTCGTT 
CGGAAGAACA GAAAGATCCA TAAAGACGCA GAATCCGCCC AGTCGTGCAC AGACTCCAGC 
GGAAGCTTCG CCAAGCTGAA CGGCCTCTTT GACAGCCCCG TCAAGGAATA CCAGCAGAAC 
ATTGATTCTC CCAAACTCTA CAGCAACCTG CTGACCAGTC GGAAGGAACT GCCACCAAAC 
ACGGATACAA AGTCCATGGC CGTGGACCAC AGAGGCCAGC CTCCCGAGCT GGCTGCTCTC 
CCCACGCCGG AATCCACACC TGTCCTCCAC CAGAAGACCC TGCAGGCCAT GAAGAGCCAC 
TCTGAGAAGG CCCACAGCCA CGGTGCTTCA AGGAAAGAAC ACCCCCAGTT TTTTCCTTCT 
AGTCCTCCAC CCCATTCCCC ATTGAGTCAC GGGCATATCC CCAGTGCCAT CGTTCTTCCA 
AACGCCACTC ACGACTACAA TACATCCTTC TCCAACTCGA ATGCCCACAA AGCCGAAAAG 
AAGCTTCAGA GCATGGATCA CCCTCTTACG AAGTCATCCA GTAAGCGGGA GCACCGGCGG 
TCTGTGGATT CCAGGAATAC TCTCAATGAT CTCCTGAAGC ATCTAAATGA CCCAAACAGT 
AACCCCAAAG CCATCCTGGG AGAGATCCAT ATGGCTCATC AAACCCTCAT GCTGGACCCG 
GTGGGACCAA TGGCTGAGGT CCCACCCAAG GTCCCTAACC GGGAGGCATC TCTATACTCC 
CCTCCCTCCA CACTCCCCAG AAATAGTCCA ACCAAGAGAG TAGATGTCCC CACCACTCCT 
GGGGTGCCAA TGACTTCTCT GGAAAGACAA AGGGGTTATC ACAAAAATTC CTCCCAGAGG 
CACTCTATAT CTGCCGTGCC TAAAAACTTA AACTCACCAA ATGGTGTTTT GTTATCTAGA 
CAGCCGAGTA TGAACCGTGG AGGCTATATG CCCACCCCAA CAGGGGCGAA GGTGGACTAT 
ATTCAGGGGA CACCGGTGAG TGTTCATCTG CAGCCCTCCC TCTCCAGACA GAGCAGCTAT 
ACCAGTAATG GCACCCTCCC CAGGACGGGA CTAAAGAGGA CACCATCCTT AAAACCTGAT 
GTGCCACCAA AGCCTTCCTT TGTTCCGCAA ACCACATCTG TCAGACCACT GAACAAGTAC 
ACGTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172484.1
CDS	832..4080
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_017316995.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316995.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGAAA GTTTGCCTCC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCCTC ATCTTCTGTT ACCACTGTGG CAAGTAGCCC AGAAATTACA 
TCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ATACTTCTGA TTTTACTGAT ACTATATCAG GTGTACGGTG GGAAGTCCAG 
TCTGGAGAAT CCAATCAGAT GGTCCACATG AATGTCCTCA TCACCTGCGT GTTTGCCGCT 
TTTGTCTTGG GCGCGTTCAT CGCAGGAGTG GCCGTGTACT GCTACCGTGA CATGTTCGTT 
CGGAAGAACA GAAAGATCCA TAAAGACGCA GAATCCGCCC AGTCGTGCAC AGACTCCAGC 
GGAAGCTTCG CCAAGCTGAA CGGCCTCTTT GACAGCCCCG TCAAGGAATA CCAGCAGAAC 
ATTGATTCTC CCAAACTCTA CAGCAACCTG CTGACCAGTC GGAAGGAACT GCCACCAAAC 
ACGGATACAA AGTCCATGGC CGTGGACCAC AGAGGCCAGC CTCCCGAGCT GGCTGCTCTC 
CCCACGCCGG AATCCACACC TGTCCTCCAC CAGAAGACCC TGCAGGCCAT GAAGAGCCAC 
TCTGAGAAGG CCCACAGCCA CGGTGCTTCA AGGAAAGAAC ACCCCCAGTT TTTTCCTTCT 
AGTCCTCCAC CCCATTCCCC ATTGAGTCAC GGGCATATCC CCAGTGCCAT CGTTCTTCCA 
AACGCCACTC ACGACTACAA TACATCCTTC TCCAACTCGA ATGCCCACAA AGCCGAAAAG 
AAGCTTCAGA GCATGGATCA CCCTCTTACG AAGTCATCCA GTAAGCGGGA GCACCGGCGG 
TCTGTGGATT CCAGGAATAC TCTCAATGAT CTCCTGAAGC ATCTAAATGA CCCAAACAGT 
AACCCCAAAG CCATCCTGGG AGAGATCCAT ATGGCTCATC AAACCCTCAT GCTGGACCCG 
GTGGGACCAA TGGCTGAGGT CCCACCCAAG GTCCCTAACC GGGAGGCATC TCTATACTCC 
CCTCCCTCCA CACTCCCCAG AAATAGTCCA ACCAAGAGAG TAGATGTCCC CACCACTCCT 
GGGGTGCCAA TGACTTCTCT GGAAAGACAA AGGGGTTATC ACAAAAATTC CTCCCAGAGG 
CACTCTATAT CTGCCGTGCC TAAAAACTTA AACTCACCAA ATGGTGTTTT GTTATCTAGA 
CAGCCGAGTA TGAACCGTGG AGGCTATATG CCCACCCCAA CAGGGGCGAA GGTGGACTAT 
ATTCAGGGGA CACCGGTGAG TGTTCATCTG CAGCCCTCCC TCTCCAGACA GAGCAGCTAT 
ACCAGTAATG GCACCCTCCC CAGGACGGGA CTAAAGAGGA CACCATCCTT AAAACCTGAT 
GTGCCACCAA AGCCTTCCTT TGTTCCGCAA ACCACATCTG TCAGACCACT GAACAAGTAC 
ACGTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_017317010.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172499.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu72912
Clone ID Related Accession (Same CDS sequence)	XM_030249357.1 , XM_017317010.1 , XM_017317010.2
Accession Version	XM_017317010.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGACA TGGAGGTATC CTCATCTTCT GTTACCACTG TGGCAAGTAG CCCAGAAATT 
ACATCTAAAG TGATTGATAC CTGGAGACCT AAACTGACGA GCTCCCGGAA ATTTGTAGTT 
CAAGATGACC CAAATACTTC TGATTTTACT GATACTATAT CAGGTATCCC AAAGGGTGTA 
CGGTGGGAAG TCCAGTCTGG AGAATCCAAT CAGATGGTCC ACATGAATGT CCTCATCACC 
TGCGTGTTTG CCGCTTTTGT CTTGGGCGCG TTCATCGCAG GAGTGGCCGT GTACTGCTAC 
CGTGACATGT TCGTTCGGAA GAACAGAAAG ATCCATAAAG ACGCAGAATC CGCCCAGTCG 
TGCACAGACT CCAGCGGAAG CTTCGCCAAG CTGAACGGCC TCTTTGACAG CCCCGTCAAG 
GAATACCAGC AGAACATTGA TTCTCCCAAA CTCTACAGCA ACCTGCTGAC CAGTCGGAAG 
GAACTGCCAC CAAACACGGA TACAAAGTCC ATGGCCGTGG ACCACAGAGG CCAGCCTCCC 
GAGCTGGCTG CTCTCCCCAC GCCGGAATCC ACACCTGTCC TCCACCAGAA GACCCTGCAG 
GCCATGAAGA GCCACTCTGA GAAGGCCCAC AGCCACGGTG CTTCAAGGAA AGAACACCCC 
CAGTTTTTTC CTTCTAGTCC TCCACCCCAT TCCCCATTGA GTCACGGGCA TATCCCCAGT 
GCCATCGTTC TTCCAAACGC CACTCACGAC TACAATACAT CCTTCTCCAA CTCGAATGCC 
CACAAAGCCG AAAAGAAGCT TCAGAGCATG GATCACCCTC TTACGAAGTC ATCCAGTAAG 
CGGGAGCACC GGCGGTCTGT GGATTCCAGG AATACTCTCA ATGATCTCCT GAAGCATCTA 
AATGACCCAA ACAGTAACCC CAAAGCCATC CTGGGAGAGA TCCATATGGC TCATCAAACC 
CTCATGCTGG ACCCGGTGGG ACCAATGGCT GAGGTCCCAC CCAAGGTCCC TAACCGGGAG 
GCATCTCTAT ACTCCCCTCC CTCCACACTC CCCAGAAATA GTCCAACCAA GAGAGTAGAT 
GTCCCCACCA CTCCTGGGGT GCCAATGACT TCTCTGGAAA GACAAAGGGG TTATCACAAA 
AATTCCTCCC AGAGGCACTC TATATCTGCC GTGCCTAAAA ACTTAAACTC ACCAAATGGT 
GTTTTGTTAT CTAGACAGCC GAGTATGAAC CGTGGAGGCT ATATGCCCAC CCCAACAGGG 
GCGAAGGTGG ACTATATTCA GGGGACACCG GTGAGTGTTC ATCTGCAGCC CTCCCTCTCC 
AGACAGAGCA GCTATACCAG TAATGGCACC CTCCCCAGGA CGGGACTAAA GAGGACACCA 
TCCTTAAAAC CTGATGTGCC ACCAAAGCCT TCCTTTGTTC CGCAAACCAC ATCTGTCAGA 
CCACTGAACA AGTACACGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172499.1
CDS	832..4035
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSPGGYEQDTEYGNTAHLGDCHDMEVSSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_017317010.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317010.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC 
CACAGCCCTG GAGGATATGA GCAGGACACG GAGTACGGCA ACACAGCCCA CCTAGGGGAC 
TGCCACGACA TGGAGGTATC CTCATCTTCT GTTACCACTG TGGCAAGTAG CCCAGAAATT 
ACATCTAAAG TGATTGATAC CTGGAGACCT AAACTGACGA GCTCCCGGAA ATTTGTAGTT 
CAAGATGACC CAAATACTTC TGATTTTACT GATACTATAT CAGGTATCCC AAAGGGTGTA 
CGGTGGGAAG TCCAGTCTGG AGAATCCAAT CAGATGGTCC ACATGAATGT CCTCATCACC 
TGCGTGTTTG CCGCTTTTGT CTTGGGCGCG TTCATCGCAG GAGTGGCCGT GTACTGCTAC 
CGTGACATGT TCGTTCGGAA GAACAGAAAG ATCCATAAAG ACGCAGAATC CGCCCAGTCG 
TGCACAGACT CCAGCGGAAG CTTCGCCAAG CTGAACGGCC TCTTTGACAG CCCCGTCAAG 
GAATACCAGC AGAACATTGA TTCTCCCAAA CTCTACAGCA ACCTGCTGAC CAGTCGGAAG 
GAACTGCCAC CAAACACGGA TACAAAGTCC ATGGCCGTGG ACCACAGAGG CCAGCCTCCC 
GAGCTGGCTG CTCTCCCCAC GCCGGAATCC ACACCTGTCC TCCACCAGAA GACCCTGCAG 
GCCATGAAGA GCCACTCTGA GAAGGCCCAC AGCCACGGTG CTTCAAGGAA AGAACACCCC 
CAGTTTTTTC CTTCTAGTCC TCCACCCCAT TCCCCATTGA GTCACGGGCA TATCCCCAGT 
GCCATCGTTC TTCCAAACGC CACTCACGAC TACAATACAT CCTTCTCCAA CTCGAATGCC 
CACAAAGCCG AAAAGAAGCT TCAGAGCATG GATCACCCTC TTACGAAGTC ATCCAGTAAG 
CGGGAGCACC GGCGGTCTGT GGATTCCAGG AATACTCTCA ATGATCTCCT GAAGCATCTA 
AATGACCCAA ACAGTAACCC CAAAGCCATC CTGGGAGAGA TCCATATGGC TCATCAAACC 
CTCATGCTGG ACCCGGTGGG ACCAATGGCT GAGGTCCCAC CCAAGGTCCC TAACCGGGAG 
GCATCTCTAT ACTCCCCTCC CTCCACACTC CCCAGAAATA GTCCAACCAA GAGAGTAGAT 
GTCCCCACCA CTCCTGGGGT GCCAATGACT TCTCTGGAAA GACAAAGGGG TTATCACAAA 
AATTCCTCCC AGAGGCACTC TATATCTGCC GTGCCTAAAA ACTTAAACTC ACCAAATGGT 
GTTTTGTTAT CTAGACAGCC GAGTATGAAC CGTGGAGGCT ATATGCCCAC CCCAACAGGG 
GCGAAGGTGG ACTATATTCA GGGGACACCG GTGAGTGTTC ATCTGCAGCC CTCCCTCTCC 
AGACAGAGCA GCTATACCAG TAATGGCACC CTCCCCAGGA CGGGACTAAA GAGGACACCA 
TCCTTAAAAC CTGATGTGCC ACCAAAGCCT TCCTTTGTTC CGCAAACCAC ATCTGTCAGA 
CCACTGAACA AGTACACGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_017317021.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172510.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X12, mRNA.

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CloneID	OMu72913
Clone ID Related Accession (Same CDS sequence)	XM_017317021.2 , XM_030249368.1 , XM_017317021.1
Accession Version	XM_017317021.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	semaphorin-6D isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGGTGTA CGGTGGGAAG TCCAGTCTGG AGAATCCAAT 
CAGATGGTCC ACATGAATGT CCTCATCACC TGCGTGTTTG CCGCTTTTGT CTTGGGCGCG 
TTCATCGCAG GAGTGGCCGT GTACTGCTAC CGTGACATGT TCGTTCGGAA GAACAGAAAG 
ATCCATAAAG ACGCAGAATC CGCCCAGTCG TGCACAGACT CCAGCGGAAG CTTCGCCAAG 
CTGAACGGCC TCTTTGACAG CCCCGTCAAG GAATACCAGC AGAACATTGA TTCTCCCAAA 
CTCTACAGCA ACCTGCTGAC CAGTCGGAAG GAACTGCCAC CAAACACGGA TACAAAGTCC 
ATGGCCGTGG ACCACAGAGG CCAGCCTCCC GAGCTGGCTG CTCTCCCCAC GCCGGAATCC 
ACACCTGTCC TCCACCAGAA GACCCTGCAG GCCATGAAGA GCCACTCTGA GAAGGCCCAC 
AGCCACGGTG CTTCAAGGAA AGAACACCCC CAGTTTTTTC CTTCTAGTCC TCCACCCCAT 
TCCCCATTGA GTCACGGGCA TATCCCCAGT GCCATCGTTC TTCCAAACGC CACTCACGAC 
TACAATACAT CCTTCTCCAA CTCGAATGCC CACAAAGCCG AAAAGAAGCT TCAGAGCATG 
GATCACCCTC TTACGAAGTC ATCCAGTAAG CGGGAGCACC GGCGGTCTGT GGATTCCAGG 
AATACTCTCA ATGATCTCCT GAAGCATCTA AATGACCCAA ACAGTAACCC CAAAGCCATC 
CTGGGAGAGA TCCATATGGC TCATCAAACC CTCATGCTGG ACCCGGTGGG ACCAATGGCT 
GAGGTCCCAC CCAAGGTCCC TAACCGGGAG GCATCTCTAT ACTCCCCTCC CTCCACACTC 
CCCAGAAATA GTCCAACCAA GAGAGTAGAT GTCCCCACCA CTCCTGGGGT GCCAATGACT 
TCTCTGGAAA GACAAAGGGG TTATCACAAA AATTCCTCCC AGAGGCACTC TATATCTGCC 
GTGCCTAAAA ACTTAAACTC ACCAAATGGT GTTTTGTTAT CTAGACAGCC GAGTATGAAC 
CGTGGAGGCT ATATGCCCAC CCCAACAGGG GCGAAGGTGG ACTATATTCA GGGGACACCG 
GTGAGTGTTC ATCTGCAGCC CTCCCTCTCC AGACAGAGCA GCTATACCAG TAATGGCACC 
CTCCCCAGGA CGGGACTAAA GAGGACACCA TCCTTAAAAC CTGATGTGCC ACCAAAGCCT 
TCCTTTGTTC CGCAAACCAC ATCTGTCAGA CCACTGAACA AGTACACGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172510.1
CDS	832..3885
Translation	MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYGNTAHLGDCHESLPPSTTPDYKIFGGPTSGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPMAEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_017317021.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317021.1

ORF Insert Sequence:

ATGGGGTTCC TTCTGCTTTG GTTCTGCGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCG 
GTCAGCTTCC CAGAAGACGA TGAGCCCCTC AACACGGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG AAATCCCCCA AACAGAGGTG ATACCAAGCA AGAAGCTGAC GTGGAGGTCC 
AGACAGCAGG ATCGAGAAAA TTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTCATCAAAG TCTTTGTCCC AAGAAATGAT GAGATGGTTT TTGTCTGTGG TACCAATGCT 
TTCAACCCGA TGTGCAGATA CTATAGGTTG AGAACGTTAG AGTATGATGG GGAAGAAATT 
AGTGGCCTGG CACGATGCCC GTTTGATGCC CGACAAACCA ATGTCGCCCT CTTTGCTGAT 
GGAAAACTCT ATTCTGCCAC AGTGGCTGAT TTCCTGGCCA GTGATGCTGT CATTTACAGA 
AGCATGGGAG ATGGATCTGC CCTTCGCACA ATAAAATACG ATTCCAAGTG GATCAAAGAA 
CCACACTTCC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT CAGAGAAATC 
GCCGTGGAAC ATAATAACTT AGGCAAGGCT GTGTATTCCC GCGTGGCTCG CATTTGTAAA 
AACGACATGG GTGGCTCACA GCGGGTCCTG GAGAAACACT GGACTTCCTT CCTTAAGGCT 
CGGCTGAACT GCTCCGTTCC TGGAGATTCC TTTTTCTACT TCGACGTCCT GCAGTCTATA 
ACAGACATAA TCCAAATCAA TGGCATCCCC ACTGTGGTTG GGGTCTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCTGC AGTCTGTGCC TTTAGCATGG ACGACATTGA GAAAGTGTTC 
AAAGGGCGGT TCAAAGAGCA GAAAACCCCA GACTCTGTTT GGACAGCAGT TCCCGAAGAC 
AAAGTACCAA AACCAAGGCC TGGCTGTTGT GCCAAACACG GCCTCGCAGA AGCTTACAAG 
ACCTCCATCG ACTTTCCAGA TGACACCCTG GCTTTCATCA AGTCCCACCC GCTGATGGAC 
TCTGCCGTCC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACACGGGT CAGGTACAGG 
TTGACAGCCA TCGAAGTGGA CCGTTCAGCA GGGCCATACC AAAACTACAC AGTCATCTTT 
GTTGGCTCTG AAGCTGGCGT GGTACTTAAA GTTTTGGCAA AGACCAGTCC TTTCTCTCTG 
AATGACAGTG TATTACTCGA AGAGATTGAA GCTTATAACC CAGCCAAGTG CAGCGCCGAG 
AGTGAGGAGG ACAGAAAGGT GGTCTCATTA CAGCTGGACA AGGATCACCA TGCTTTATAC 
GTGGCCTTCT CTAGCTGCGT GGTCCGCATC CCCCTCAGCC GCTGTGAGCG CTACGGATCG 
TGTAAAAAGT CTTGCATTGC ATCACGTGAC CCGTACTGTG GTTGGTTAAG CCAGGGAGTT 
TGTGAGAGAG TGACCCTAGG GATGCTCCCT GGAGGATATG AGCAGGACAC GGAGTACGGC 
AACACAGCCC ACCTAGGGGA CTGCCACGAA AGTTTGCCTC CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGGTGTA CGGTGGGAAG TCCAGTCTGG AGAATCCAAT 
CAGATGGTCC ACATGAATGT CCTCATCACC TGCGTGTTTG CCGCTTTTGT CTTGGGCGCG 
TTCATCGCAG GAGTGGCCGT GTACTGCTAC CGTGACATGT TCGTTCGGAA GAACAGAAAG 
ATCCATAAAG ACGCAGAATC CGCCCAGTCG TGCACAGACT CCAGCGGAAG CTTCGCCAAG 
CTGAACGGCC TCTTTGACAG CCCCGTCAAG GAATACCAGC AGAACATTGA TTCTCCCAAA 
CTCTACAGCA ACCTGCTGAC CAGTCGGAAG GAACTGCCAC CAAACACGGA TACAAAGTCC 
ATGGCCGTGG ACCACAGAGG CCAGCCTCCC GAGCTGGCTG CTCTCCCCAC GCCGGAATCC 
ACACCTGTCC TCCACCAGAA GACCCTGCAG GCCATGAAGA GCCACTCTGA GAAGGCCCAC 
AGCCACGGTG CTTCAAGGAA AGAACACCCC CAGTTTTTTC CTTCTAGTCC TCCACCCCAT 
TCCCCATTGA GTCACGGGCA TATCCCCAGT GCCATCGTTC TTCCAAACGC CACTCACGAC 
TACAATACAT CCTTCTCCAA CTCGAATGCC CACAAAGCCG AAAAGAAGCT TCAGAGCATG 
GATCACCCTC TTACGAAGTC ATCCAGTAAG CGGGAGCACC GGCGGTCTGT GGATTCCAGG 
AATACTCTCA ATGATCTCCT GAAGCATCTA AATGACCCAA ACAGTAACCC CAAAGCCATC 
CTGGGAGAGA TCCATATGGC TCATCAAACC CTCATGCTGG ACCCGGTGGG ACCAATGGCT 
GAGGTCCCAC CCAAGGTCCC TAACCGGGAG GCATCTCTAT ACTCCCCTCC CTCCACACTC 
CCCAGAAATA GTCCAACCAA GAGAGTAGAT GTCCCCACCA CTCCTGGGGT GCCAATGACT 
TCTCTGGAAA GACAAAGGGG TTATCACAAA AATTCCTCCC AGAGGCACTC TATATCTGCC 
GTGCCTAAAA ACTTAAACTC ACCAAATGGT GTTTTGTTAT CTAGACAGCC GAGTATGAAC 
CGTGGAGGCT ATATGCCCAC CCCAACAGGG GCGAAGGTGG ACTATATTCA GGGGACACCG 
GTGAGTGTTC ATCTGCAGCC CTCCCTCTCC AGACAGAGCA GCTATACCAG TAATGGCACC 
CTCCCCAGGA CGGGACTAAA GAGGACACCA TCCTTAAAAC CTGATGTGCC ACCAAAGCCT 
TCCTTTGTTC CGCAAACCAC ATCTGTCAGA CCACTGAACA AGTACACGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcription and Signaling Regulators in Developing Neuronal Subtypes of Mouse and Human Enteric Nervous System.
Gastroenterology(2017 Oct)
Memic F,Knoflach V,Morarach K,Sadler R,Laranjeira C,Hjerling-Leffler J,Sundstr?m E,Pachnis V,Marklund U

Control of species-dependent cortico-motoneuronal connections underlying manual dexterity.
Science (New York, N.Y.)357(6349)400-404(2017 07)
Gu Z,Kalambogias J,Yoshioka S,Han W,Li Z,Kawasawa YI,Pochareddy S,Li Z,Liu F,Xu X,Wijeratne HRS,Ueno M,Blatz E,Salomone J,Kumanogoh A,Rasin MR,Gebelein B,Weirauch MT,Sestan N,Martin JH,Yoshida Y

Shear stress-regulated miR-27b controls pericyte recruitment by repressing SEMA6A and SEMA6D.
Cardiovascular research113(6)681-691(2017 May)
Demolli S,Doddaballapur A,Devraj K,Stark K,Manavski Y,Eckart A,Zehendner CM,Lucas T,Korff T,Hecker M,Massberg S,Liebner S,Kaluza D,Boon RA,Dimmeler S

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Sema6d cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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