sema6d cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following sema6d gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sema6d cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa43025 XM_018091934.1
Latest version!
Xenopus tropicalis sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (sema6d), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
OXa22115 XM_031897617.1
Latest version!
Xenopus tropicalis semaphorin 6D (sema6d), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa22115 XM_012958562.3
Latest version!
Xenopus tropicalis semaphorin 6D (sema6d), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa53240 XM_012958563.3
Latest version!
Xenopus tropicalis semaphorin 6D (sema6d), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa53241 XM_012958564.3
Latest version!
Xenopus tropicalis semaphorin 6D (sema6d), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa53242 XM_012958565.3
Latest version!
Xenopus tropicalis semaphorin 6D (sema6d), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa22115 NM_001126680.1
Latest version!
Xenopus tropicalis semaphorin 6D (sema6d), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa31618 XM_012958562.2
Latest version!
Xenopus tropicalis sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (sema6d), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa31619 XM_012958563.2
Latest version!
Xenopus tropicalis sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (sema6d), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa31620 XM_012958564.2
Latest version!
Xenopus tropicalis sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (sema6d), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa31621 XM_012958565.2
Latest version!
Xenopus tropicalis sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (sema6d), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa43025
    Clone ID Related Accession (Same CDS sequence) XM_018091934.1
    Accession Version XM_018091934.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1431bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product semaphorin-6D isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGACACTGC GCGCGGGTTG TGCCTGGGCT CTGCTGCTAA CAGTAAGCAC GGTCTGTGCC 
    GTTAGCTTTC CTGAAGACGA CGAGCCTATT AATGTTGTAG ATTATCACTA TTCAAGGCAA
    TATCCAGTAT TTAGAGGACG CCCTTCAGGC AATGATTCAC AGCACAGCCT GGACTTTCAA
    CTGATGCTGA AAATTCGAGA CACACTTTAT GTCGCTGGAA GAGATCAGGT TTATGCTATC
    AATTTAAATG AAGTACCGAA GACTGAAGTA ACACCAAGCA GAAGGTTGAC ATGGAGGTCG
    GGCCAAGCAG ACCGAAAAAA CTGTGCAATG AAAGGAAAGC ACAAGGATGA ATGCCATAAC
    TACATCAAGG TTTTCGTTCC AAGAAATGAT GAGATGGTGT TTGTCTGTGG TACAAATGCA
    TTCAATCCTA TGTGCAGATA TTACAGGTTA AATACTTTTG ATTATGATGG GGAAGAAATT
    AGTGGCTTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTAGCCCT ATTTGCAGAT
    GGCAAGTTAT ATTCTGCTAC CATGGCAGAT TTCCAAGCTA GTGACGCTGT GATCTATCGA
    AGCATGGGGG ATGGATTTGC TCTGAGGACA ATTAAATACG ACTCCAAATG GCTGAAAGAG
    CCACATTTTC TCCATGCAAT AGAATACGGA AATTATATCT ATTTCTTCTT TCGTGAAATT
    GCTGTTGAAC ATAATAATCT AGGCAAGGCC GTACACTCTC GTGTGGCACG CATATGCAAA
    AATGACTTGG GAGGCTCACA GCGAGTGCTG GAGAAACACT GGACTTCATT CGTAAAAGCG
    CGACTCAATT GCTCTGTTCC TGGGGATTCT TTCTTCTACT TTGATGTTCT GCAGTCTATC
    ACGGACATAA TAGAAATCAA TGGAATTCCC ACTGTTGTCG GTGTATTTAC CACACAGCTG
    AACAGCATCC CAGGTTCAGC AGTTTGTGCT TTCACCATGG ATGACATAGA AAAGGTATTC
    AGAGGAAGAT TTAAAGAACA AAAGACCCCA GACTCTGTTT GGACAGCTGT ACCTGAAGAC
    AGAGTACCTA AGCCAAGACC TGGTTGCTGT GCAAGACATG GTTACGGAGA AGGCTACAGG
    TCCTCTACAG AATTCCCTGA CGAAACCTTA TCTTTTATCA AGTCCCATCC ACTGATGGAT
    TCTGCCATCC CATCTATAAT TGAAGAACCT TGGTTCACTA AAACACGCGT AAGGTACAGA
    TTAACTGCCG TAGCAGTGGA CCACTTGGCA GGACCTTACT TAAACTACAC AGTTATGTTT
    GTGGGTTCTG AAGCTGGCGT TGTATTAAAG ATCCTAGCCA AGACAAACCC CTTTTCTCTA
    AATGACAGTG TATTACTGGA AGAAATTGAT GCCTACAACC GTGCAAAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947423.1
    CDS1637..3067
    Translation

    Target ORF information:

    RefSeq Version XM_018091934.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (sema6d), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091934.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGACACTGC GCGCGGGTTG TGCCTGGGCT CTGCTGCTAA CAGTAAGCAC GGTCTGTGCC 
    GTTAGCTTTC CTGAAGACGA CGAGCCTATT AATGTTGTAG ATTATCACTA TTCAAGGCAA
    TATCCAGTAT TTAGAGGACG CCCTTCAGGC AATGATTCAC AGCACAGCCT GGACTTTCAA
    CTGATGCTGA AAATTCGAGA CACACTTTAT GTCGCTGGAA GAGATCAGGT TTATGCTATC
    AATTTAAATG AAGTACCGAA GACTGAAGTA ACACCAAGCA GAAGGTTGAC ATGGAGGTCG
    GGCCAAGCAG ACCGAAAAAA CTGTGCAATG AAAGGAAAGC ACAAGGATGA ATGCCATAAC
    TACATCAAGG TTTTCGTTCC AAGAAATGAT GAGATGGTGT TTGTCTGTGG TACAAATGCA
    TTCAATCCTA TGTGCAGATA TTACAGGTTA AATACTTTTG ATTATGATGG GGAAGAAATT
    AGTGGCTTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTAGCCCT ATTTGCAGAT
    GGCAAGTTAT ATTCTGCTAC CATGGCAGAT TTCCAAGCTA GTGACGCTGT GATCTATCGA
    AGCATGGGGG ATGGATTTGC TCTGAGGACA ATTAAATACG ACTCCAAATG GCTGAAAGAG
    CCACATTTTC TCCATGCAAT AGAATACGGA AATTATATCT ATTTCTTCTT TCGTGAAATT
    GCTGTTGAAC ATAATAATCT AGGCAAGGCC GTACACTCTC GTGTGGCACG CATATGCAAA
    AATGACTTGG GAGGCTCACA GCGAGTGCTG GAGAAACACT GGACTTCATT CGTAAAAGCG
    CGACTCAATT GCTCTGTTCC TGGGGATTCT TTCTTCTACT TTGATGTTCT GCAGTCTATC
    ACGGACATAA TAGAAATCAA TGGAATTCCC ACTGTTGTCG GTGTATTTAC CACACAGCTG
    AACAGCATCC CAGGTTCAGC AGTTTGTGCT TTCACCATGG ATGACATAGA AAAGGTATTC
    AGAGGAAGAT TTAAAGAACA AAAGACCCCA GACTCTGTTT GGACAGCTGT ACCTGAAGAC
    AGAGTACCTA AGCCAAGACC TGGTTGCTGT GCAAGACATG GTTACGGAGA AGGCTACAGG
    TCCTCTACAG AATTCCCTGA CGAAACCTTA TCTTTTATCA AGTCCCATCC ACTGATGGAT
    TCTGCCATCC CATCTATAAT TGAAGAACCT TGGTTCACTA AAACACGCGT AAGGTACAGA
    TTAACTGCCG TAGCAGTGGA CCACTTGGCA GGACCTTACT TAAACTACAC AGTTATGTTT
    GTGGGTTCTG AAGCTGGCGT TGTATTAAAG ATCCTAGCCA AGACAAACCC CTTTTCTCTA
    AATGACAGTG TATTACTGGA AGAAATTGAT GCCTACAACC GTGCAAAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa22115
    Clone ID Related Accession (Same CDS sequence) XM_031897617.1 , XM_012958562.3 , NM_001126680.1
    Accession Version XM_031897617.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3255bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product semaphorin-6D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGACACTGC GCGCGGGTTG TGCCTGGGCT CTGCTGCTAA CAGTAAGCAC GGTCTGTGCC 
    GTTAGCTTTC CTGAAGACGA CGAGCCTATT AATGTTGTAG ATTATCACTA TTCAAGGCAA
    TATCCAGTAT TTAGAGGACG CCCTTCAGGC AATGATTCAC AGCACAGCCT GGACTTTCAA
    CTGATGCTGA AAATTCGAGA CACACTTTAT GTCGCTGGAA GAGATCAGGT TTATGCTATC
    AATTTAAATG AAGTACCGAA GACTGAAGTA ACACCAAGCA GAAGGTTGAC ATGGAGGTCG
    GGCCAAGCAG ACCGAAAAAA CTGTGCAATG AAAGGAAAGC ACAAGGATGA ATGCCATAAC
    TACATCAAGG TTTTCGTTCC AAGAAATGAT GAGATGGTGT TTGTCTGTGG TACAAATGCA
    TTCAATCCTA TGTGCAGATA TTACAGGTTA AATACTTTTG ATTATGATGG GGAAGAAATT
    AGTGGCTTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTAGCCCT ATTTGCAGAT
    GGCAAGTTAT ATTCTGCTAC CATGGCAGAT TTCCAAGCTA GTGACGCTGT GATCTATCGA
    AGCATGGGGG ATGGATTTGC TCTGAGGACA ATTAAATACG ACTCCAAATG GCTGAAAGAG
    CCACATTTTC TCCATGCAAT AGAATACGGA AATTATATCT ATTTCTTCTT TCGTGAAATT
    GCTGTTGAAC ATAATAATCT AGGCAAGGCC GTACACTCTC GTGTGGCACG CATATGCAAA
    AATGACTTGG GAGGCTCACA GCGAGTGCTG GAGAAACACT GGACTTCATT CGTAAAAGCG
    CGACTCAATT GCTCTGTTCC TGGGGATTCT TTCTTCTACT TTGATGTTCT GCAGTCTATC
    ACGGACATAA TAGAAATCAA TGGAATTCCC ACTGTTGTCG GTGTATTTAC CACACAGCTG
    AACAGCATCC CAGGTTCAGC AGTTTGTGCT TTCACCATGG ATGACATAGA AAAGGTATTC
    AGAGGAAGAT TTAAAGAACA AAAGACCCCA GACTCTGTTT GGACAGCTGT ACCTGAAGAC
    AGAGTACCTA AGCCAAGACC TGGTTGCTGT GCAAGACATG GTTACGGAGA AGGCTACAGG
    TCCTCTACAG AATTCCCTGA CGAAACCTTA TCTTTTATCA AGTCCCATCC ACTGATGGAT
    TCTGCCATCC CATCTATAAT TGAAGAACCT TGGTTCACTA AAACACGCGT AAGGTACAGA
    TTAACTGCCG TAGCAGTGGA CCACTTGGCA GGACCTTACT TAAACTACAC AGTTATGTTT
    GTGGGTTCTG AAGCTGGCGT TGTATTAAAG ATCCTAGCCA AGACAAACCC CTTTTCTCTA
    AATGACAGTG TATTACTGGA AGAAATTGAT GCCTACAACC GTGCAAAGTG TTCTGGAAAT
    AGCGAGGAGG ACAGAAAAGT TATTTCCCTT CAGATGGACA GAGATCATCA TGCCTTGTTT
    GTGGCTTTCT CTGGCTGCAT TGTCAGACTG CCTTTGAGCC GCTGTGAGCG ATACGGATCA
    TGCAAAAAGT CTTGTATTGG TGCAAGGGAT CCTTATTGTG GCTGGCTGAA CCATGAGTCC
    TGCGGAAGAG TGAAACCGGG CATGTTTTTT GGGTATGAAC AGGATGTGGA ATACGGCAAC
    ACAGTACAGC TTGGCGACTG TCATGACATT TTGCCTACTG CAACTACACC AGATTACAAA
    ATATTTGGCG ATCCAACATC TGACATTGAA GTTCCTTCTA CTTCTGTAAC CACCACAAGT
    AGCCCAGTTA TTTTACCTAA AGTGATTGGT TCCTGGAAAC CCAGAATGAC TGGCTCACGG
    AAATTTATAT ACCAAGATGA CCCAAACACC TCAGAGTATA TTGATCCGTT AACAGGTGTC
    CAAAAGGGTG TACGGTGGGA AGTACAGTCA GGAGACGCCA ACCAAATGGT CCATATGAAT
    GTTTTGATCA CCTGCGTTCT TGCTGCTTTT GTGCTCGGAG CTTTCATCGC AGGAGTGATA
    GTTTACTGCT ACAGGGAGAT GTTTGTAAGG AAAACAAGAA AAATCCACAA AGATGCAGAG
    TCTGCTCAGT CCTGTACAGA TTCAAGTGGA AGTTTTGCAA AATTAAATGG ATTGTTTGAT
    AGTCCAGTTA AAGAATATCA TCAAAGCATT GATTCTCCCA AGCACTACAC AAATATATTA
    ACTAGTAGAA AGGAACTGCC ACCAAATGGA GAAACCAAGT CTATGATGAT GGACAGTCAA
    GGACAGCCAC CTGAACTAGC GGCCCTTCCA ACACCCGAAT CTACCCCAGT TCTTCACCAA
    AAGAGTTTGC AGCCCATAAA AAACCAGTGG GAGAAAACAC ACAATAACTT AAACATCTCA
    CGGAAAGAAA CACCTCTTAA AAGTCCTCAG TTCTTTCCTT CCAGCCCTCC ACCCCATTCA
    CCTTTAGCTC ATGGGCAAAT ACCAAGTGCT GTTGTTATTC CAAATGCCAC CCATGACTAC
    AATACTTCCT TTTCAAACTC AAATGCCCAC AAATCGGATA AAAAATTGCA TAACCTGGAG
    CACTCTTTGG GAAAACAGTC AAGCAAAAGA GACCACAGGC GCTCTGTTGA TTCAAGGAAT
    ACATTAAATG ACTTTTTGAA GCATTTGAAT GAATCTGCTG GAAACCCCAA AGCTCTTATG
    GGAGATATTC AAGTAACACA TCCGAATGGT CACCAAGTTC CACATCAAAC TTTAATGTTA
    GATACAATGG GCAACATGTC TGAAGTTCCA CCTAAAGTTC CAAACAGAGA GGCATCTCTG
    TATTCCCCAT CGTCTACTCT ACCAAGAAAC AGCCCAACAA AAAGAGTAGA TGTACCGTCA
    ACTCCAAATG GGCAAATGAC TCCTATAGAA TCTCAAAGGA GCTATCATAA GAACTCTTCA
    CAGAGGCATT CAATATCAGC CATGCCTAAA AATATGAACT CACCAAATGG TGTTTTGCTT
    TCAAGACAGC CAAGTATTAA TCGAGGAGGG TATATGCCTC CAAGTCCCAG CACAAGGATG
    GACTATATGC AAGGGACGCC TATAATAGTG CACCAACAGA CTTCTTTGTC ACGGCAAAGT
    AGTTATACAG GGCATGGAAC CCTTCCTCGC ATGGGGGTAA AAAGGACACC ATCTATTAAG
    CCAGATGTGC CACCAAAACC TTCATTTACT TCACAAAATA CATCTGTAAG ACAGCTGAAC
    AAATATAGCT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753477.1
    CDS330..3584
    Translation

    Target ORF information:

    RefSeq Version XM_031897617.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis semaphorin 6D (sema6d), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897617.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGACACTGC GCGCGGGTTG TGCCTGGGCT CTGCTGCTAA CAGTAAGCAC GGTCTGTGCC 
    GTTAGCTTTC CTGAAGACGA CGAGCCTATT AATGTTGTAG ATTATCACTA TTCAAGGCAA
    TATCCAGTAT TTAGAGGACG CCCTTCAGGC AATGATTCAC AGCACAGCCT GGACTTTCAA
    CTGATGCTGA AAATTCGAGA CACACTTTAT GTCGCTGGAA GAGATCAGGT TTATGCTATC
    AATTTAAATG AAGTACCGAA GACTGAAGTA ACACCAAGCA GAAGGTTGAC ATGGAGGTCG
    GGCCAAGCAG ACCGAAAAAA CTGTGCAATG AAAGGAAAGC ACAAGGATGA ATGCCATAAC
    TACATCAAGG TTTTCGTTCC AAGAAATGAT GAGATGGTGT TTGTCTGTGG TACAAATGCA
    TTCAATCCTA TGTGCAGATA TTACAGGTTA AATACTTTTG ATTATGATGG GGAAGAAATT
    AGTGGCTTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTAGCCCT ATTTGCAGAT
    GGCAAGTTAT ATTCTGCTAC CATGGCAGAT TTCCAAGCTA GTGACGCTGT GATCTATCGA
    AGCATGGGGG ATGGATTTGC TCTGAGGACA ATTAAATACG ACTCCAAATG GCTGAAAGAG
    CCACATTTTC TCCATGCAAT AGAATACGGA AATTATATCT ATTTCTTCTT TCGTGAAATT
    GCTGTTGAAC ATAATAATCT AGGCAAGGCC GTACACTCTC GTGTGGCACG CATATGCAAA
    AATGACTTGG GAGGCTCACA GCGAGTGCTG GAGAAACACT GGACTTCATT CGTAAAAGCG
    CGACTCAATT GCTCTGTTCC TGGGGATTCT TTCTTCTACT TTGATGTTCT GCAGTCTATC
    ACGGACATAA TAGAAATCAA TGGAATTCCC ACTGTTGTCG GTGTATTTAC CACACAGCTG
    AACAGCATCC CAGGTTCAGC AGTTTGTGCT TTCACCATGG ATGACATAGA AAAGGTATTC
    AGAGGAAGAT TTAAAGAACA AAAGACCCCA GACTCTGTTT GGACAGCTGT ACCTGAAGAC
    AGAGTACCTA AGCCAAGACC TGGTTGCTGT GCAAGACATG GTTACGGAGA AGGCTACAGG
    TCCTCTACAG AATTCCCTGA CGAAACCTTA TCTTTTATCA AGTCCCATCC ACTGATGGAT
    TCTGCCATCC CATCTATAAT TGAAGAACCT TGGTTCACTA AAACACGCGT AAGGTACAGA
    TTAACTGCCG TAGCAGTGGA CCACTTGGCA GGACCTTACT TAAACTACAC AGTTATGTTT
    GTGGGTTCTG AAGCTGGCGT TGTATTAAAG ATCCTAGCCA AGACAAACCC CTTTTCTCTA
    AATGACAGTG TATTACTGGA AGAAATTGAT GCCTACAACC GTGCAAAGTG TTCTGGAAAT
    AGCGAGGAGG ACAGAAAAGT TATTTCCCTT CAGATGGACA GAGATCATCA TGCCTTGTTT
    GTGGCTTTCT CTGGCTGCAT TGTCAGACTG CCTTTGAGCC GCTGTGAGCG ATACGGATCA
    TGCAAAAAGT CTTGTATTGG TGCAAGGGAT CCTTATTGTG GCTGGCTGAA CCATGAGTCC
    TGCGGAAGAG TGAAACCGGG CATGTTTTTT GGGTATGAAC AGGATGTGGA ATACGGCAAC
    ACAGTACAGC TTGGCGACTG TCATGACATT TTGCCTACTG CAACTACACC AGATTACAAA
    ATATTTGGCG ATCCAACATC TGACATTGAA GTTCCTTCTA CTTCTGTAAC CACCACAAGT
    AGCCCAGTTA TTTTACCTAA AGTGATTGGT TCCTGGAAAC CCAGAATGAC TGGCTCACGG
    AAATTTATAT ACCAAGATGA CCCAAACACC TCAGAGTATA TTGATCCGTT AACAGGTGTC
    CAAAAGGGTG TACGGTGGGA AGTACAGTCA GGAGACGCCA ACCAAATGGT CCATATGAAT
    GTTTTGATCA CCTGCGTTCT TGCTGCTTTT GTGCTCGGAG CTTTCATCGC AGGAGTGATA
    GTTTACTGCT ACAGGGAGAT GTTTGTAAGG AAAACAAGAA AAATCCACAA AGATGCAGAG
    TCTGCTCAGT CCTGTACAGA TTCAAGTGGA AGTTTTGCAA AATTAAATGG ATTGTTTGAT
    AGTCCAGTTA AAGAATATCA TCAAAGCATT GATTCTCCCA AGCACTACAC AAATATATTA
    ACTAGTAGAA AGGAACTGCC ACCAAATGGA GAAACCAAGT CTATGATGAT GGACAGTCAA
    GGACAGCCAC CTGAACTAGC GGCCCTTCCA ACACCCGAAT CTACCCCAGT TCTTCACCAA
    AAGAGTTTGC AGCCCATAAA AAACCAGTGG GAGAAAACAC ACAATAACTT AAACATCTCA
    CGGAAAGAAA CACCTCTTAA AAGTCCTCAG TTCTTTCCTT CCAGCCCTCC ACCCCATTCA
    CCTTTAGCTC ATGGGCAAAT ACCAAGTGCT GTTGTTATTC CAAATGCCAC CCATGACTAC
    AATACTTCCT TTTCAAACTC AAATGCCCAC AAATCGGATA AAAAATTGCA TAACCTGGAG
    CACTCTTTGG GAAAACAGTC AAGCAAAAGA GACCACAGGC GCTCTGTTGA TTCAAGGAAT
    ACATTAAATG ACTTTTTGAA GCATTTGAAT GAATCTGCTG GAAACCCCAA AGCTCTTATG
    GGAGATATTC AAGTAACACA TCCGAATGGT CACCAAGTTC CACATCAAAC TTTAATGTTA
    GATACAATGG GCAACATGTC TGAAGTTCCA CCTAAAGTTC CAAACAGAGA GGCATCTCTG
    TATTCCCCAT CGTCTACTCT ACCAAGAAAC AGCCCAACAA AAAGAGTAGA TGTACCGTCA
    ACTCCAAATG GGCAAATGAC TCCTATAGAA TCTCAAAGGA GCTATCATAA GAACTCTTCA
    CAGAGGCATT CAATATCAGC CATGCCTAAA AATATGAACT CACCAAATGG TGTTTTGCTT
    TCAAGACAGC CAAGTATTAA TCGAGGAGGG TATATGCCTC CAAGTCCCAG CACAAGGATG
    GACTATATGC AAGGGACGCC TATAATAGTG CACCAACAGA CTTCTTTGTC ACGGCAAAGT
    AGTTATACAG GGCATGGAAC CCTTCCTCGC ATGGGGGTAA AAAGGACACC ATCTATTAAG
    CCAGATGTGC CACCAAAACC TTCATTTACT TCACAAAATA CATCTGTAAG ACAGCTGAAC
    AAATATAGCT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa22115
    Clone ID Related Accession (Same CDS sequence) XM_031897617.1 , XM_012958562.3 , NM_001126680.1
    Accession Version XM_012958562.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3255bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product semaphorin-6D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958562.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGACACTGC GCGCGGGTTG TGCCTGGGCT CTGCTGCTAA CAGTAAGCAC GGTCTGTGCC 
    GTTAGCTTTC CTGAAGACGA CGAGCCTATT AATGTTGTAG ATTATCACTA TTCAAGGCAA
    TATCCAGTAT TTAGAGGACG CCCTTCAGGC AATGATTCAC AGCACAGCCT GGACTTTCAA
    CTGATGCTGA AAATTCGAGA CACACTTTAT GTCGCTGGAA GAGATCAGGT TTATGCTATC
    AATTTAAATG AAGTACCGAA GACTGAAGTA ACACCAAGCA GAAGGTTGAC ATGGAGGTCG
    GGCCAAGCAG ACCGAAAAAA CTGTGCAATG AAAGGAAAGC ACAAGGATGA ATGCCATAAC
    TACATCAAGG TTTTCGTTCC AAGAAATGAT GAGATGGTGT TTGTCTGTGG TACAAATGCA
    TTCAATCCTA TGTGCAGATA TTACAGGTTA AATACTTTTG ATTATGATGG GGAAGAAATT
    AGTGGCTTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTAGCCCT ATTTGCAGAT
    GGCAAGTTAT ATTCTGCTAC CATGGCAGAT TTCCAAGCTA GTGACGCTGT GATCTATCGA
    AGCATGGGGG ATGGATTTGC TCTGAGGACA ATTAAATACG ACTCCAAATG GCTGAAAGAG
    CCACATTTTC TCCATGCAAT AGAATACGGA AATTATATCT ATTTCTTCTT TCGTGAAATT
    GCTGTTGAAC ATAATAATCT AGGCAAGGCC GTACACTCTC GTGTGGCACG CATATGCAAA
    AATGACTTGG GAGGCTCACA GCGAGTGCTG GAGAAACACT GGACTTCATT CGTAAAAGCG
    CGACTCAATT GCTCTGTTCC TGGGGATTCT TTCTTCTACT TTGATGTTCT GCAGTCTATC
    ACGGACATAA TAGAAATCAA TGGAATTCCC ACTGTTGTCG GTGTATTTAC CACACAGCTG
    AACAGCATCC CAGGTTCAGC AGTTTGTGCT TTCACCATGG ATGACATAGA AAAGGTATTC
    AGAGGAAGAT TTAAAGAACA AAAGACCCCA GACTCTGTTT GGACAGCTGT ACCTGAAGAC
    AGAGTACCTA AGCCAAGACC TGGTTGCTGT GCAAGACATG GTTACGGAGA AGGCTACAGG
    TCCTCTACAG AATTCCCTGA CGAAACCTTA TCTTTTATCA AGTCCCATCC ACTGATGGAT
    TCTGCCATCC CATCTATAAT TGAAGAACCT TGGTTCACTA AAACACGCGT AAGGTACAGA
    TTAACTGCCG TAGCAGTGGA CCACTTGGCA GGACCTTACT TAAACTACAC AGTTATGTTT
    GTGGGTTCTG AAGCTGGCGT TGTATTAAAG ATCCTAGCCA AGACAAACCC CTTTTCTCTA
    AATGACAGTG TATTACTGGA AGAAATTGAT GCCTACAACC GTGCAAAGTG TTCTGGAAAT
    AGCGAGGAGG ACAGAAAAGT TATTTCCCTT CAGATGGACA GAGATCATCA TGCCTTGTTT
    GTGGCTTTCT CTGGCTGCAT TGTCAGACTG CCTTTGAGCC GCTGTGAGCG ATACGGATCA
    TGCAAAAAGT CTTGTATTGG TGCAAGGGAT CCTTATTGTG GCTGGCTGAA CCATGAGTCC
    TGCGGAAGAG TGAAACCGGG CATGTTTTTT GGGTATGAAC AGGATGTGGA ATACGGCAAC
    ACAGTACAGC TTGGCGACTG TCATGACATT TTGCCTACTG CAACTACACC AGATTACAAA
    ATATTTGGCG ATCCAACATC TGACATTGAA GTTCCTTCTA CTTCTGTAAC CACCACAAGT
    AGCCCAGTTA TTTTACCTAA AGTGATTGGT TCCTGGAAAC CCAGAATGAC TGGCTCACGG
    AAATTTATAT ACCAAGATGA CCCAAACACC TCAGAGTATA TTGATCCGTT AACAGGTGTC
    CAAAAGGGTG TACGGTGGGA AGTACAGTCA GGAGACGCCA ACCAAATGGT CCATATGAAT
    GTTTTGATCA CCTGCGTTCT TGCTGCTTTT GTGCTCGGAG CTTTCATCGC AGGAGTGATA
    GTTTACTGCT ACAGGGAGAT GTTTGTAAGG AAAACAAGAA AAATCCACAA AGATGCAGAG
    TCTGCTCAGT CCTGTACAGA TTCAAGTGGA AGTTTTGCAA AATTAAATGG ATTGTTTGAT
    AGTCCAGTTA AAGAATATCA TCAAAGCATT GATTCTCCCA AGCACTACAC AAATATATTA
    ACTAGTAGAA AGGAACTGCC ACCAAATGGA GAAACCAAGT CTATGATGAT GGACAGTCAA
    GGACAGCCAC CTGAACTAGC GGCCCTTCCA ACACCCGAAT CTACCCCAGT TCTTCACCAA
    AAGAGTTTGC AGCCCATAAA AAACCAGTGG GAGAAAACAC ACAATAACTT AAACATCTCA
    CGGAAAGAAA CACCTCTTAA AAGTCCTCAG TTCTTTCCTT CCAGCCCTCC ACCCCATTCA
    CCTTTAGCTC ATGGGCAAAT ACCAAGTGCT GTTGTTATTC CAAATGCCAC CCATGACTAC
    AATACTTCCT TTTCAAACTC AAATGCCCAC AAATCGGATA AAAAATTGCA TAACCTGGAG
    CACTCTTTGG GAAAACAGTC AAGCAAAAGA GACCACAGGC GCTCTGTTGA TTCAAGGAAT
    ACATTAAATG ACTTTTTGAA GCATTTGAAT GAATCTGCTG GAAACCCCAA AGCTCTTATG
    GGAGATATTC AAGTAACACA TCCGAATGGT CACCAAGTTC CACATCAAAC TTTAATGTTA
    GATACAATGG GCAACATGTC TGAAGTTCCA CCTAAAGTTC CAAACAGAGA GGCATCTCTG
    TATTCCCCAT CGTCTACTCT ACCAAGAAAC AGCCCAACAA AAAGAGTAGA TGTACCGTCA
    ACTCCAAATG GGCAAATGAC TCCTATAGAA TCTCAAAGGA GCTATCATAA GAACTCTTCA
    CAGAGGCATT CAATATCAGC CATGCCTAAA AATATGAACT CACCAAATGG TGTTTTGCTT
    TCAAGACAGC CAAGTATTAA TCGAGGAGGG TATATGCCTC CAAGTCCCAG CACAAGGATG
    GACTATATGC AAGGGACGCC TATAATAGTG CACCAACAGA CTTCTTTGTC ACGGCAAAGT
    AGTTATACAG GGCATGGAAC CCTTCCTCGC ATGGGGGTAA AAAGGACACC ATCTATTAAG
    CCAGATGTGC CACCAAAACC TTCATTTACT TCACAAAATA CATCTGTAAG ACAGCTGAAC
    AAATATAGCT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814016.1
    CDS80..3334
    Translation

    Target ORF information:

    RefSeq Version XM_012958562.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis semaphorin 6D (sema6d), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958562.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGACACTGC GCGCGGGTTG TGCCTGGGCT CTGCTGCTAA CAGTAAGCAC GGTCTGTGCC 
    GTTAGCTTTC CTGAAGACGA CGAGCCTATT AATGTTGTAG ATTATCACTA TTCAAGGCAA
    TATCCAGTAT TTAGAGGACG CCCTTCAGGC AATGATTCAC AGCACAGCCT GGACTTTCAA
    CTGATGCTGA AAATTCGAGA CACACTTTAT GTCGCTGGAA GAGATCAGGT TTATGCTATC
    AATTTAAATG AAGTACCGAA GACTGAAGTA ACACCAAGCA GAAGGTTGAC ATGGAGGTCG
    GGCCAAGCAG ACCGAAAAAA CTGTGCAATG AAAGGAAAGC ACAAGGATGA ATGCCATAAC
    TACATCAAGG TTTTCGTTCC AAGAAATGAT GAGATGGTGT TTGTCTGTGG TACAAATGCA
    TTCAATCCTA TGTGCAGATA TTACAGGTTA AATACTTTTG ATTATGATGG GGAAGAAATT
    AGTGGCTTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTAGCCCT ATTTGCAGAT
    GGCAAGTTAT ATTCTGCTAC CATGGCAGAT TTCCAAGCTA GTGACGCTGT GATCTATCGA
    AGCATGGGGG ATGGATTTGC TCTGAGGACA ATTAAATACG ACTCCAAATG GCTGAAAGAG
    CCACATTTTC TCCATGCAAT AGAATACGGA AATTATATCT ATTTCTTCTT TCGTGAAATT
    GCTGTTGAAC ATAATAATCT AGGCAAGGCC GTACACTCTC GTGTGGCACG CATATGCAAA
    AATGACTTGG GAGGCTCACA GCGAGTGCTG GAGAAACACT GGACTTCATT CGTAAAAGCG
    CGACTCAATT GCTCTGTTCC TGGGGATTCT TTCTTCTACT TTGATGTTCT GCAGTCTATC
    ACGGACATAA TAGAAATCAA TGGAATTCCC ACTGTTGTCG GTGTATTTAC CACACAGCTG
    AACAGCATCC CAGGTTCAGC AGTTTGTGCT TTCACCATGG ATGACATAGA AAAGGTATTC
    AGAGGAAGAT TTAAAGAACA AAAGACCCCA GACTCTGTTT GGACAGCTGT ACCTGAAGAC
    AGAGTACCTA AGCCAAGACC TGGTTGCTGT GCAAGACATG GTTACGGAGA AGGCTACAGG
    TCCTCTACAG AATTCCCTGA CGAAACCTTA TCTTTTATCA AGTCCCATCC ACTGATGGAT
    TCTGCCATCC CATCTATAAT TGAAGAACCT TGGTTCACTA AAACACGCGT AAGGTACAGA
    TTAACTGCCG TAGCAGTGGA CCACTTGGCA GGACCTTACT TAAACTACAC AGTTATGTTT
    GTGGGTTCTG AAGCTGGCGT TGTATTAAAG ATCCTAGCCA AGACAAACCC CTTTTCTCTA
    AATGACAGTG TATTACTGGA AGAAATTGAT GCCTACAACC GTGCAAAGTG TTCTGGAAAT
    AGCGAGGAGG ACAGAAAAGT TATTTCCCTT CAGATGGACA GAGATCATCA TGCCTTGTTT
    GTGGCTTTCT CTGGCTGCAT TGTCAGACTG CCTTTGAGCC GCTGTGAGCG ATACGGATCA
    TGCAAAAAGT CTTGTATTGG TGCAAGGGAT CCTTATTGTG GCTGGCTGAA CCATGAGTCC
    TGCGGAAGAG TGAAACCGGG CATGTTTTTT GGGTATGAAC AGGATGTGGA ATACGGCAAC
    ACAGTACAGC TTGGCGACTG TCATGACATT TTGCCTACTG CAACTACACC AGATTACAAA
    ATATTTGGCG ATCCAACATC TGACATTGAA GTTCCTTCTA CTTCTGTAAC CACCACAAGT
    AGCCCAGTTA TTTTACCTAA AGTGATTGGT TCCTGGAAAC CCAGAATGAC TGGCTCACGG
    AAATTTATAT ACCAAGATGA CCCAAACACC TCAGAGTATA TTGATCCGTT AACAGGTGTC
    CAAAAGGGTG TACGGTGGGA AGTACAGTCA GGAGACGCCA ACCAAATGGT CCATATGAAT
    GTTTTGATCA CCTGCGTTCT TGCTGCTTTT GTGCTCGGAG CTTTCATCGC AGGAGTGATA
    GTTTACTGCT ACAGGGAGAT GTTTGTAAGG AAAACAAGAA AAATCCACAA AGATGCAGAG
    TCTGCTCAGT CCTGTACAGA TTCAAGTGGA AGTTTTGCAA AATTAAATGG ATTGTTTGAT
    AGTCCAGTTA AAGAATATCA TCAAAGCATT GATTCTCCCA AGCACTACAC AAATATATTA
    ACTAGTAGAA AGGAACTGCC ACCAAATGGA GAAACCAAGT CTATGATGAT GGACAGTCAA
    GGACAGCCAC CTGAACTAGC GGCCCTTCCA ACACCCGAAT CTACCCCAGT TCTTCACCAA
    AAGAGTTTGC AGCCCATAAA AAACCAGTGG GAGAAAACAC ACAATAACTT AAACATCTCA
    CGGAAAGAAA CACCTCTTAA AAGTCCTCAG TTCTTTCCTT CCAGCCCTCC ACCCCATTCA
    CCTTTAGCTC ATGGGCAAAT ACCAAGTGCT GTTGTTATTC CAAATGCCAC CCATGACTAC
    AATACTTCCT TTTCAAACTC AAATGCCCAC AAATCGGATA AAAAATTGCA TAACCTGGAG
    CACTCTTTGG GAAAACAGTC AAGCAAAAGA GACCACAGGC GCTCTGTTGA TTCAAGGAAT
    ACATTAAATG ACTTTTTGAA GCATTTGAAT GAATCTGCTG GAAACCCCAA AGCTCTTATG
    GGAGATATTC AAGTAACACA TCCGAATGGT CACCAAGTTC CACATCAAAC TTTAATGTTA
    GATACAATGG GCAACATGTC TGAAGTTCCA CCTAAAGTTC CAAACAGAGA GGCATCTCTG
    TATTCCCCAT CGTCTACTCT ACCAAGAAAC AGCCCAACAA AAAGAGTAGA TGTACCGTCA
    ACTCCAAATG GGCAAATGAC TCCTATAGAA TCTCAAAGGA GCTATCATAA GAACTCTTCA
    CAGAGGCATT CAATATCAGC CATGCCTAAA AATATGAACT CACCAAATGG TGTTTTGCTT
    TCAAGACAGC CAAGTATTAA TCGAGGAGGG TATATGCCTC CAAGTCCCAG CACAAGGATG
    GACTATATGC AAGGGACGCC TATAATAGTG CACCAACAGA CTTCTTTGTC ACGGCAAAGT
    AGTTATACAG GGCATGGAAC CCTTCCTCGC ATGGGGGTAA AAAGGACACC ATCTATTAAG
    CCAGATGTGC CACCAAAACC TTCATTTACT TCACAAAATA CATCTGTAAG ACAGCTGAAC
    AAATATAGCT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53240
    Clone ID Related Accession (Same CDS sequence) XM_012958563.3
    Accession Version XM_012958563.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product semaphorin-6D isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958563.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACACTGC GCGCGGGTTG TGCCTGGGCT CTGCTGCTAA CAGTAAGCAC GGTCTGTGCC 
    GTTAGCTTTC CTGAAGACGA CGAGCCTATT AATGTTGTAG ATTATCACTA TTCAAGGCAA
    TATCCAGTAT TTAGAGGACG CCCTTCAGGC AATGATTCAC AGCACAGCCT GGACTTTCAA
    CTGATGCTGA AAATTCGAGA CACACTTTAT GTCGCTGGAA GAGATCAGGT TTATGCTATC
    AATTTAAATG AAGTACCGAA GACTGAAGTA ACACCAAGCA GAAGGTTGAC ATGGAGGTCG
    GGCCAAGCAG ACCGAAAAAA CTGTGCAATG AAAGGAAAGC ACAAGGATGA ATGCCATAAC
    TACATCAAGG TTTTCGTTCC AAGAAATGAT GAGATGGTGT TTGTCTGTGG TACAAATGCA
    TTCAATCCTA TGTGCAGATA TTACAGGTTA AATACTTTTG ATTATGATGG GGAAGAAATT
    AGTGGCTTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTAGCCCT ATTTGCAGAT
    GGCAAGTTAT ATTCTGCTAC CATGGCAGAT TTCCAAGCTA GTGACGCTGT GATCTATCGA
    AGCATGGGGG ATGGATTTGC TCTGAGGACA ATTAAATACG ACTCCAAATG GCTGAAAGAG
    CCACATTTTC TCCATGCAAT AGAATACGGA AATTATATCT ATTTCTTCTT TCGTGAAATT
    GCTGTTGAAC ATAATAATCT AGGCAAGGCC GTACACTCTC GTGTGGCACG CATATGCAAA
    AATGACTTGG GAGGCTCACA GCGAGTGCTG GAGAAACACT GGACTTCATT CGTAAAAGCG
    CGACTCAATT GCTCTGTTCC TGGGGATTCT TTCTTCTACT TTGATGTTCT GCAGTCTATC
    ACGGACATAA TAGAAATCAA TGGAATTCCC ACTGTTGTCG GTGTATTTAC CACACAGCTG
    AACAGCATCC CAGGTTCAGC AGTTTGTGCT TTCACCATGG ATGACATAGA AAAGGTATTC
    AGAGGAAGAT TTAAAGAACA AAAGACCCCA GACTCTGTTT GGACAGCTGT ACCTGAAGAC
    AGAGTACCTA AGCCAAGACC TGGTTGCTGT GCAAGACATG GTTACGGAGA AGGCTACAGG
    TCCTCTACAG AATTCCCTGA CGAAACCTTA TCTTTTATCA AGTCCCATCC ACTGATGGAT
    TCTGCCATCC CATCTATAAT TGAAGAACCT TGGTTCACTA AAACACGCGT AAGGTACAGA
    TTAACTGCCG TAGCAGTGGA CCACTTGGCA GGACCTTACT TAAACTACAC AGTTATGTTT
    GTGGGTTCTG AAGCTGGCGT TGTATTAAAG ATCCTAGCCA AGACAAACCC CTTTTCTCTA
    AATGACAGTG TATTACTGGA AGAAATTGAT GCCTACAACC GTGCAAAGTG TTCTGGAAAT
    AGCGAGGAGG ACAGAAAAGT TATTTCCCTT CAGATGGACA GAGATCATCA TGCCTTGTTT
    GTGGCTTTCT CTGGCTGCAT TGTCAGACTG CCTTTGAGCC GCTGTGAGCG ATACGGATCA
    TGCAAAAAGT CTTGTATTGG TGCAAGGGAT CCTTATTGTG GCTGGCTGAA CCATGAGTCC
    TGCGGAAGAG TGAAACCGGG CATGTTTTTT GGGTATGAAC AGGATGTGGA ATACGGCAAC
    ACAGTACAGC TTGGCGACTG TCATGACATT GAAGTTCCTT CTACTTCTGT AACCACCACA
    AGTAGCCCAG TTATTTTACC TAAAGTGATT GGTTCCTGGA AACCCAGAAT GACTGGCTCA
    CGGAAATTTA TATACCAAGA TGACCCAAAC ACCTCAGAGT ATATTGATCC GTTAACAGGT
    GTCCAAAAGG GTGTACGGTG GGAAGTACAG TCAGGAGACG CCAACCAAAT GGTCCATATG
    AATGTTTTGA TCACCTGCGT TCTTGCTGCT TTTGTGCTCG GAGCTTTCAT CGCAGGAGTG
    ATAGTTTACT GCTACAGGGA GATGTTTGTA AGGAAAACAA GAAAAATCCA CAAAGATGCA
    GAGTCTGCTC AGTCCTGTAC AGATTCAAGT GGAAGTTTTG CAAAATTAAA TGGATTGTTT
    GATAGTCCAG TTAAAGAATA TCATCAAAGC ATTGATTCTC CCAAGCACTA CACAAATATA
    TTAACTAGTA GAAAGGAACT GCCACCAAAT GGAGAAACCA AGTCTATGAT GATGGACAGT
    CAAGGACAGC CACCTGAACT AGCGGCCCTT CCAACACCCG AATCTACCCC AGTTCTTCAC
    CAAAAGAGTT TGCAGCCCAT AAAAAACCAG TGGGAGAAAA CACACAATAA CTTAAACATC
    TCACGGAAAG AAACACCTCT TAAAAGTCCT CAGTTCTTTC CTTCCAGCCC TCCACCCCAT
    TCACCTTTAG CTCATGGGCA AATACCAAGT GCTGTTGTTA TTCCAAATGC CACCCATGAC
    TACAATACTT CCTTTTCAAA CTCAAATGCC CACAAATCGG ATAAAAAATT GCATAACCTG
    GAGCACTCTT TGGGAAAACA GTCAAGCAAA AGAGACCACA GGCGCTCTGT TGATTCAAGG
    AATACATTAA ATGACTTTTT GAAGCATTTG AATGAATCTG CTGGAAACCC CAAAGCTCTT
    ATGGGAGATA TTCAAGTAAC ACATCCGAAT GGTCACCAAG TTCCACATCA AACTTTAATG
    TTAGATACAA TGGGCAACAT GTCTGAAGTT CCACCTAAAG TTCCAAACAG AGAGGCATCT
    CTGTATTCCC CATCGTCTAC TCTACCAAGA AACAGCCCAA CAAAAAGAGT AGATGTACCG
    TCAACTCCAA ATGGGCAAAT GACTCCTATA GAATCTCAAA GGAGCTATCA TAAGAACTCT
    TCACAGAGGC ATTCAATATC AGCCATGCCT AAAAATATGA ACTCACCAAA TGGTGTTTTG
    CTTTCAAGAC AGCCAAGTAT TAATCGAGGA GGGTATATGC CTCCAAGTCC CAGCACAAGG
    ATGGACTATA TGCAAGGGAC GCCTATAATA GTGCACCAAC AGACTTCTTT GTCACGGCAA
    AGTAGTTATA CAGGGCATGG AACCCTTCCT CGCATGGGGG TAAAAAGGAC ACCATCTATT
    AAGCCAGATG TGCCACCAAA ACCTTCATTT ACTTCACAAA ATACATCTGT AAGACAGCTG
    AACAAATATA GCTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814017.1
    CDS940..4137
    Translation

    Target ORF information:

    RefSeq Version XM_012958563.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis semaphorin 6D (sema6d), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958563.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACACTGC GCGCGGGTTG TGCCTGGGCT CTGCTGCTAA CAGTAAGCAC GGTCTGTGCC 
    GTTAGCTTTC CTGAAGACGA CGAGCCTATT AATGTTGTAG ATTATCACTA TTCAAGGCAA
    TATCCAGTAT TTAGAGGACG CCCTTCAGGC AATGATTCAC AGCACAGCCT GGACTTTCAA
    CTGATGCTGA AAATTCGAGA CACACTTTAT GTCGCTGGAA GAGATCAGGT TTATGCTATC
    AATTTAAATG AAGTACCGAA GACTGAAGTA ACACCAAGCA GAAGGTTGAC ATGGAGGTCG
    GGCCAAGCAG ACCGAAAAAA CTGTGCAATG AAAGGAAAGC ACAAGGATGA ATGCCATAAC
    TACATCAAGG TTTTCGTTCC AAGAAATGAT GAGATGGTGT TTGTCTGTGG TACAAATGCA
    TTCAATCCTA TGTGCAGATA TTACAGGTTA AATACTTTTG ATTATGATGG GGAAGAAATT
    AGTGGCTTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTAGCCCT ATTTGCAGAT
    GGCAAGTTAT ATTCTGCTAC CATGGCAGAT TTCCAAGCTA GTGACGCTGT GATCTATCGA
    AGCATGGGGG ATGGATTTGC TCTGAGGACA ATTAAATACG ACTCCAAATG GCTGAAAGAG
    CCACATTTTC TCCATGCAAT AGAATACGGA AATTATATCT ATTTCTTCTT TCGTGAAATT
    GCTGTTGAAC ATAATAATCT AGGCAAGGCC GTACACTCTC GTGTGGCACG CATATGCAAA
    AATGACTTGG GAGGCTCACA GCGAGTGCTG GAGAAACACT GGACTTCATT CGTAAAAGCG
    CGACTCAATT GCTCTGTTCC TGGGGATTCT TTCTTCTACT TTGATGTTCT GCAGTCTATC
    ACGGACATAA TAGAAATCAA TGGAATTCCC ACTGTTGTCG GTGTATTTAC CACACAGCTG
    AACAGCATCC CAGGTTCAGC AGTTTGTGCT TTCACCATGG ATGACATAGA AAAGGTATTC
    AGAGGAAGAT TTAAAGAACA AAAGACCCCA GACTCTGTTT GGACAGCTGT ACCTGAAGAC
    AGAGTACCTA AGCCAAGACC TGGTTGCTGT GCAAGACATG GTTACGGAGA AGGCTACAGG
    TCCTCTACAG AATTCCCTGA CGAAACCTTA TCTTTTATCA AGTCCCATCC ACTGATGGAT
    TCTGCCATCC CATCTATAAT TGAAGAACCT TGGTTCACTA AAACACGCGT AAGGTACAGA
    TTAACTGCCG TAGCAGTGGA CCACTTGGCA GGACCTTACT TAAACTACAC AGTTATGTTT
    GTGGGTTCTG AAGCTGGCGT TGTATTAAAG ATCCTAGCCA AGACAAACCC CTTTTCTCTA
    AATGACAGTG TATTACTGGA AGAAATTGAT GCCTACAACC GTGCAAAGTG TTCTGGAAAT
    AGCGAGGAGG ACAGAAAAGT TATTTCCCTT CAGATGGACA GAGATCATCA TGCCTTGTTT
    GTGGCTTTCT CTGGCTGCAT TGTCAGACTG CCTTTGAGCC GCTGTGAGCG ATACGGATCA
    TGCAAAAAGT CTTGTATTGG TGCAAGGGAT CCTTATTGTG GCTGGCTGAA CCATGAGTCC
    TGCGGAAGAG TGAAACCGGG CATGTTTTTT GGGTATGAAC AGGATGTGGA ATACGGCAAC
    ACAGTACAGC TTGGCGACTG TCATGACATT GAAGTTCCTT CTACTTCTGT AACCACCACA
    AGTAGCCCAG TTATTTTACC TAAAGTGATT GGTTCCTGGA AACCCAGAAT GACTGGCTCA
    CGGAAATTTA TATACCAAGA TGACCCAAAC ACCTCAGAGT ATATTGATCC GTTAACAGGT
    GTCCAAAAGG GTGTACGGTG GGAAGTACAG TCAGGAGACG CCAACCAAAT GGTCCATATG
    AATGTTTTGA TCACCTGCGT TCTTGCTGCT TTTGTGCTCG GAGCTTTCAT CGCAGGAGTG
    ATAGTTTACT GCTACAGGGA GATGTTTGTA AGGAAAACAA GAAAAATCCA CAAAGATGCA
    GAGTCTGCTC AGTCCTGTAC AGATTCAAGT GGAAGTTTTG CAAAATTAAA TGGATTGTTT
    GATAGTCCAG TTAAAGAATA TCATCAAAGC ATTGATTCTC CCAAGCACTA CACAAATATA
    TTAACTAGTA GAAAGGAACT GCCACCAAAT GGAGAAACCA AGTCTATGAT GATGGACAGT
    CAAGGACAGC CACCTGAACT AGCGGCCCTT CCAACACCCG AATCTACCCC AGTTCTTCAC
    CAAAAGAGTT TGCAGCCCAT AAAAAACCAG TGGGAGAAAA CACACAATAA CTTAAACATC
    TCACGGAAAG AAACACCTCT TAAAAGTCCT CAGTTCTTTC CTTCCAGCCC TCCACCCCAT
    TCACCTTTAG CTCATGGGCA AATACCAAGT GCTGTTGTTA TTCCAAATGC CACCCATGAC
    TACAATACTT CCTTTTCAAA CTCAAATGCC CACAAATCGG ATAAAAAATT GCATAACCTG
    GAGCACTCTT TGGGAAAACA GTCAAGCAAA AGAGACCACA GGCGCTCTGT TGATTCAAGG
    AATACATTAA ATGACTTTTT GAAGCATTTG AATGAATCTG CTGGAAACCC CAAAGCTCTT
    ATGGGAGATA TTCAAGTAAC ACATCCGAAT GGTCACCAAG TTCCACATCA AACTTTAATG
    TTAGATACAA TGGGCAACAT GTCTGAAGTT CCACCTAAAG TTCCAAACAG AGAGGCATCT
    CTGTATTCCC CATCGTCTAC TCTACCAAGA AACAGCCCAA CAAAAAGAGT AGATGTACCG
    TCAACTCCAA ATGGGCAAAT GACTCCTATA GAATCTCAAA GGAGCTATCA TAAGAACTCT
    TCACAGAGGC ATTCAATATC AGCCATGCCT AAAAATATGA ACTCACCAAA TGGTGTTTTG
    CTTTCAAGAC AGCCAAGTAT TAATCGAGGA GGGTATATGC CTCCAAGTCC CAGCACAAGG
    ATGGACTATA TGCAAGGGAC GCCTATAATA GTGCACCAAC AGACTTCTTT GTCACGGCAA
    AGTAGTTATA CAGGGCATGG AACCCTTCCT CGCATGGGGG TAAAAAGGAC ACCATCTATT
    AAGCCAGATG TGCCACCAAA ACCTTCATTT ACTTCACAAA ATACATCTGT AAGACAGCTG
    AACAAATATA GCTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53241
    Clone ID Related Accession (Same CDS sequence) XM_012958564.3
    Accession Version XM_012958564.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3090bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product semaphorin-6D isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958564.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGACACTGC GCGCGGGTTG TGCCTGGGCT CTGCTGCTAA CAGTAAGCAC GGTCTGTGCC 
    GTTAGCTTTC CTGAAGACGA CGAGCCTATT AATGTTGTAG ATTATCACTA TTCAAGGCAA
    TATCCAGTAT TTAGAGGACG CCCTTCAGGC AATGATTCAC AGCACAGCCT GGACTTTCAA
    CTGATGCTGA AAATTCGAGA CACACTTTAT GTCGCTGGAA GAGATCAGGT TTATGCTATC
    AATTTAAATG AAGTACCGAA GACTGAAGTA ACACCAAGCA GAAGGTTGAC ATGGAGGTCG
    GGCCAAGCAG ACCGAAAAAA CTGTGCAATG AAAGGAAAGC ACAAGGATGA ATGCCATAAC
    TACATCAAGG TTTTCGTTCC AAGAAATGAT GAGATGGTGT TTGTCTGTGG TACAAATGCA
    TTCAATCCTA TGTGCAGATA TTACAGGTTA AATACTTTTG ATTATGATGG GGAAGAAATT
    AGTGGCTTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTAGCCCT ATTTGCAGAT
    GGCAAGTTAT ATTCTGCTAC CATGGCAGAT TTCCAAGCTA GTGACGCTGT GATCTATCGA
    AGCATGGGGG ATGGATTTGC TCTGAGGACA ATTAAATACG ACTCCAAATG GCTGAAAGAG
    CCACATTTTC TCCATGCAAT AGAATACGGA AATTATATCT ATTTCTTCTT TCGTGAAATT
    GCTGTTGAAC ATAATAATCT AGGCAAGGCC GTACACTCTC GTGTGGCACG CATATGCAAA
    AATGACTTGG GAGGCTCACA GCGAGTGCTG GAGAAACACT GGACTTCATT CGTAAAAGCG
    CGACTCAATT GCTCTGTTCC TGGGGATTCT TTCTTCTACT TTGATGTTCT GCAGTCTATC
    ACGGACATAA TAGAAATCAA TGGAATTCCC ACTGTTGTCG GTGTATTTAC CACACAGCTG
    AACAGCATCC CAGGTTCAGC AGTTTGTGCT TTCACCATGG ATGACATAGA AAAGGTATTC
    AGAGGAAGAT TTAAAGAACA AAAGACCCCA GACTCTGTTT GGACAGCTGT ACCTGAAGAC
    AGAGTACCTA AGCCAAGACC TGGTTGCTGT GCAAGACATG GTTACGGAGA AGGCTACAGG
    TCCTCTACAG AATTCCCTGA CGAAACCTTA TCTTTTATCA AGTCCCATCC ACTGATGGAT
    TCTGCCATCC CATCTATAAT TGAAGAACCT TGGTTCACTA AAACACGCGT AAGGTACAGA
    TTAACTGCCG TAGCAGTGGA CCACTTGGCA GGACCTTACT TAAACTACAC AGTTATGTTT
    GTGGGTTCTG AAGCTGGCGT TGTATTAAAG ATCCTAGCCA AGACAAACCC CTTTTCTCTA
    AATGACAGTG TATTACTGGA AGAAATTGAT GCCTACAACC GTGCAAAGTG TTCTGGAAAT
    AGCGAGGAGG ACAGAAAAGT TATTTCCCTT CAGATGGACA GAGATCATCA TGCCTTGTTT
    GTGGCTTTCT CTGGCTGCAT TGTCAGACTG CCTTTGAGCC GCTGTGAGCG ATACGGATCA
    TGCAAAAAGT CTTGTATTGG TGCAAGGGAT CCTTATTGTG GCTGGCTGAA CCATGAGTCC
    TGCGGAAGAG TGAAACCGGG CATGTTTTTT GGGTATGAAC AGGATGTGGA ATACGGCAAC
    ACAGTACAGC TTGGCGACTG TCATGACATT TTGCCTACTG CAACTACACC AGATTACAAA
    ATATTTGGCG ATCCAACATC TGGTGTACGG TGGGAAGTAC AGTCAGGAGA CGCCAACCAA
    ATGGTCCATA TGAATGTTTT GATCACCTGC GTTCTTGCTG CTTTTGTGCT CGGAGCTTTC
    ATCGCAGGAG TGATAGTTTA CTGCTACAGG GAGATGTTTG TAAGGAAAAC AAGAAAAATC
    CACAAAGATG CAGAGTCTGC TCAGTCCTGT ACAGATTCAA GTGGAAGTTT TGCAAAATTA
    AATGGATTGT TTGATAGTCC AGTTAAAGAA TATCATCAAA GCATTGATTC TCCCAAGCAC
    TACACAAATA TATTAACTAG TAGAAAGGAA CTGCCACCAA ATGGAGAAAC CAAGTCTATG
    ATGATGGACA GTCAAGGACA GCCACCTGAA CTAGCGGCCC TTCCAACACC CGAATCTACC
    CCAGTTCTTC ACCAAAAGAG TTTGCAGCCC ATAAAAAACC AGTGGGAGAA AACACACAAT
    AACTTAAACA TCTCACGGAA AGAAACACCT CTTAAAAGTC CTCAGTTCTT TCCTTCCAGC
    CCTCCACCCC ATTCACCTTT AGCTCATGGG CAAATACCAA GTGCTGTTGT TATTCCAAAT
    GCCACCCATG ACTACAATAC TTCCTTTTCA AACTCAAATG CCCACAAATC GGATAAAAAA
    TTGCATAACC TGGAGCACTC TTTGGGAAAA CAGTCAAGCA AAAGAGACCA CAGGCGCTCT
    GTTGATTCAA GGAATACATT AAATGACTTT TTGAAGCATT TGAATGAATC TGCTGGAAAC
    CCCAAAGCTC TTATGGGAGA TATTCAAGTA ACACATCCGA ATGGTCACCA AGTTCCACAT
    CAAACTTTAA TGTTAGATAC AATGGGCAAC ATGTCTGAAG TTCCACCTAA AGTTCCAAAC
    AGAGAGGCAT CTCTGTATTC CCCATCGTCT ACTCTACCAA GAAACAGCCC AACAAAAAGA
    GTAGATGTAC CGTCAACTCC AAATGGGCAA ATGACTCCTA TAGAATCTCA AAGGAGCTAT
    CATAAGAACT CTTCACAGAG GCATTCAATA TCAGCCATGC CTAAAAATAT GAACTCACCA
    AATGGTGTTT TGCTTTCAAG ACAGCCAAGT ATTAATCGAG GAGGGTATAT GCCTCCAAGT
    CCCAGCACAA GGATGGACTA TATGCAAGGG ACGCCTATAA TAGTGCACCA ACAGACTTCT
    TTGTCACGGC AAAGTAGTTA TACAGGGCAT GGAACCCTTC CTCGCATGGG GGTAAAAAGG
    ACACCATCTA TTAAGCCAGA TGTGCCACCA AAACCTTCAT TTACTTCACA AAATACATCT
    GTAAGACAGC TGAACAAATA TAGCTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814018.1
    CDS940..4029
    Translation

    Target ORF information:

    RefSeq Version XM_012958564.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis semaphorin 6D (sema6d), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958564.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGACACTGC GCGCGGGTTG TGCCTGGGCT CTGCTGCTAA CAGTAAGCAC GGTCTGTGCC 
    GTTAGCTTTC CTGAAGACGA CGAGCCTATT AATGTTGTAG ATTATCACTA TTCAAGGCAA
    TATCCAGTAT TTAGAGGACG CCCTTCAGGC AATGATTCAC AGCACAGCCT GGACTTTCAA
    CTGATGCTGA AAATTCGAGA CACACTTTAT GTCGCTGGAA GAGATCAGGT TTATGCTATC
    AATTTAAATG AAGTACCGAA GACTGAAGTA ACACCAAGCA GAAGGTTGAC ATGGAGGTCG
    GGCCAAGCAG ACCGAAAAAA CTGTGCAATG AAAGGAAAGC ACAAGGATGA ATGCCATAAC
    TACATCAAGG TTTTCGTTCC AAGAAATGAT GAGATGGTGT TTGTCTGTGG TACAAATGCA
    TTCAATCCTA TGTGCAGATA TTACAGGTTA AATACTTTTG ATTATGATGG GGAAGAAATT
    AGTGGCTTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTAGCCCT ATTTGCAGAT
    GGCAAGTTAT ATTCTGCTAC CATGGCAGAT TTCCAAGCTA GTGACGCTGT GATCTATCGA
    AGCATGGGGG ATGGATTTGC TCTGAGGACA ATTAAATACG ACTCCAAATG GCTGAAAGAG
    CCACATTTTC TCCATGCAAT AGAATACGGA AATTATATCT ATTTCTTCTT TCGTGAAATT
    GCTGTTGAAC ATAATAATCT AGGCAAGGCC GTACACTCTC GTGTGGCACG CATATGCAAA
    AATGACTTGG GAGGCTCACA GCGAGTGCTG GAGAAACACT GGACTTCATT CGTAAAAGCG
    CGACTCAATT GCTCTGTTCC TGGGGATTCT TTCTTCTACT TTGATGTTCT GCAGTCTATC
    ACGGACATAA TAGAAATCAA TGGAATTCCC ACTGTTGTCG GTGTATTTAC CACACAGCTG
    AACAGCATCC CAGGTTCAGC AGTTTGTGCT TTCACCATGG ATGACATAGA AAAGGTATTC
    AGAGGAAGAT TTAAAGAACA AAAGACCCCA GACTCTGTTT GGACAGCTGT ACCTGAAGAC
    AGAGTACCTA AGCCAAGACC TGGTTGCTGT GCAAGACATG GTTACGGAGA AGGCTACAGG
    TCCTCTACAG AATTCCCTGA CGAAACCTTA TCTTTTATCA AGTCCCATCC ACTGATGGAT
    TCTGCCATCC CATCTATAAT TGAAGAACCT TGGTTCACTA AAACACGCGT AAGGTACAGA
    TTAACTGCCG TAGCAGTGGA CCACTTGGCA GGACCTTACT TAAACTACAC AGTTATGTTT
    GTGGGTTCTG AAGCTGGCGT TGTATTAAAG ATCCTAGCCA AGACAAACCC CTTTTCTCTA
    AATGACAGTG TATTACTGGA AGAAATTGAT GCCTACAACC GTGCAAAGTG TTCTGGAAAT
    AGCGAGGAGG ACAGAAAAGT TATTTCCCTT CAGATGGACA GAGATCATCA TGCCTTGTTT
    GTGGCTTTCT CTGGCTGCAT TGTCAGACTG CCTTTGAGCC GCTGTGAGCG ATACGGATCA
    TGCAAAAAGT CTTGTATTGG TGCAAGGGAT CCTTATTGTG GCTGGCTGAA CCATGAGTCC
    TGCGGAAGAG TGAAACCGGG CATGTTTTTT GGGTATGAAC AGGATGTGGA ATACGGCAAC
    ACAGTACAGC TTGGCGACTG TCATGACATT TTGCCTACTG CAACTACACC AGATTACAAA
    ATATTTGGCG ATCCAACATC TGGTGTACGG TGGGAAGTAC AGTCAGGAGA CGCCAACCAA
    ATGGTCCATA TGAATGTTTT GATCACCTGC GTTCTTGCTG CTTTTGTGCT CGGAGCTTTC
    ATCGCAGGAG TGATAGTTTA CTGCTACAGG GAGATGTTTG TAAGGAAAAC AAGAAAAATC
    CACAAAGATG CAGAGTCTGC TCAGTCCTGT ACAGATTCAA GTGGAAGTTT TGCAAAATTA
    AATGGATTGT TTGATAGTCC AGTTAAAGAA TATCATCAAA GCATTGATTC TCCCAAGCAC
    TACACAAATA TATTAACTAG TAGAAAGGAA CTGCCACCAA ATGGAGAAAC CAAGTCTATG
    ATGATGGACA GTCAAGGACA GCCACCTGAA CTAGCGGCCC TTCCAACACC CGAATCTACC
    CCAGTTCTTC ACCAAAAGAG TTTGCAGCCC ATAAAAAACC AGTGGGAGAA AACACACAAT
    AACTTAAACA TCTCACGGAA AGAAACACCT CTTAAAAGTC CTCAGTTCTT TCCTTCCAGC
    CCTCCACCCC ATTCACCTTT AGCTCATGGG CAAATACCAA GTGCTGTTGT TATTCCAAAT
    GCCACCCATG ACTACAATAC TTCCTTTTCA AACTCAAATG CCCACAAATC GGATAAAAAA
    TTGCATAACC TGGAGCACTC TTTGGGAAAA CAGTCAAGCA AAAGAGACCA CAGGCGCTCT
    GTTGATTCAA GGAATACATT AAATGACTTT TTGAAGCATT TGAATGAATC TGCTGGAAAC
    CCCAAAGCTC TTATGGGAGA TATTCAAGTA ACACATCCGA ATGGTCACCA AGTTCCACAT
    CAAACTTTAA TGTTAGATAC AATGGGCAAC ATGTCTGAAG TTCCACCTAA AGTTCCAAAC
    AGAGAGGCAT CTCTGTATTC CCCATCGTCT ACTCTACCAA GAAACAGCCC AACAAAAAGA
    GTAGATGTAC CGTCAACTCC AAATGGGCAA ATGACTCCTA TAGAATCTCA AAGGAGCTAT
    CATAAGAACT CTTCACAGAG GCATTCAATA TCAGCCATGC CTAAAAATAT GAACTCACCA
    AATGGTGTTT TGCTTTCAAG ACAGCCAAGT ATTAATCGAG GAGGGTATAT GCCTCCAAGT
    CCCAGCACAA GGATGGACTA TATGCAAGGG ACGCCTATAA TAGTGCACCA ACAGACTTCT
    TTGTCACGGC AAAGTAGTTA TACAGGGCAT GGAACCCTTC CTCGCATGGG GGTAAAAAGG
    ACACCATCTA TTAAGCCAGA TGTGCCACCA AAACCTTCAT TTACTTCACA AAATACATCT
    GTAAGACAGC TGAACAAATA TAGCTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53242
    Clone ID Related Accession (Same CDS sequence) XM_012958565.3
    Accession Version XM_012958565.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3033bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product semaphorin-6D isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958565.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGACACTGC GCGCGGGTTG TGCCTGGGCT CTGCTGCTAA CAGTAAGCAC GGTCTGTGCC 
    GTTAGCTTTC CTGAAGACGA CGAGCCTATT AATGTTGTAG ATTATCACTA TTCAAGGCAA
    TATCCAGTAT TTAGAGGACG CCCTTCAGGC AATGATTCAC AGCACAGCCT GGACTTTCAA
    CTGATGCTGA AAATTCGAGA CACACTTTAT GTCGCTGGAA GAGATCAGGT TTATGCTATC
    AATTTAAATG AAGTACCGAA GACTGAAGTA ACACCAAGCA GAAGGTTGAC ATGGAGGTCG
    GGCCAAGCAG ACCGAAAAAA CTGTGCAATG AAAGGAAAGC ACAAGGATGA ATGCCATAAC
    TACATCAAGG TTTTCGTTCC AAGAAATGAT GAGATGGTGT TTGTCTGTGG TACAAATGCA
    TTCAATCCTA TGTGCAGATA TTACAGGTTA AATACTTTTG ATTATGATGG GGAAGAAATT
    AGTGGCTTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTAGCCCT ATTTGCAGAT
    GGCAAGTTAT ATTCTGCTAC CATGGCAGAT TTCCAAGCTA GTGACGCTGT GATCTATCGA
    AGCATGGGGG ATGGATTTGC TCTGAGGACA ATTAAATACG ACTCCAAATG GCTGAAAGAG
    CCACATTTTC TCCATGCAAT AGAATACGGA AATTATATCT ATTTCTTCTT TCGTGAAATT
    GCTGTTGAAC ATAATAATCT AGGCAAGGCC GTACACTCTC GTGTGGCACG CATATGCAAA
    AATGACTTGG GAGGCTCACA GCGAGTGCTG GAGAAACACT GGACTTCATT CGTAAAAGCG
    CGACTCAATT GCTCTGTTCC TGGGGATTCT TTCTTCTACT TTGATGTTCT GCAGTCTATC
    ACGGACATAA TAGAAATCAA TGGAATTCCC ACTGTTGTCG GTGTATTTAC CACACAGCTG
    AACAGCATCC CAGGTTCAGC AGTTTGTGCT TTCACCATGG ATGACATAGA AAAGGTATTC
    AGAGGAAGAT TTAAAGAACA AAAGACCCCA GACTCTGTTT GGACAGCTGT ACCTGAAGAC
    AGAGTACCTA AGCCAAGACC TGGTTGCTGT GCAAGACATG GTTACGGAGA AGGCTACAGG
    TCCTCTACAG AATTCCCTGA CGAAACCTTA TCTTTTATCA AGTCCCATCC ACTGATGGAT
    TCTGCCATCC CATCTATAAT TGAAGAACCT TGGTTCACTA AAACACGCGT AAGGTACAGA
    TTAACTGCCG TAGCAGTGGA CCACTTGGCA GGACCTTACT TAAACTACAC AGTTATGTTT
    GTGGGTTCTG AAGCTGGCGT TGTATTAAAG ATCCTAGCCA AGACAAACCC CTTTTCTCTA
    AATGACAGTG TATTACTGGA AGAAATTGAT GCCTACAACC GTGCAAAGTG TTCTGGAAAT
    AGCGAGGAGG ACAGAAAAGT TATTTCCCTT CAGATGGACA GAGATCATCA TGCCTTGTTT
    GTGGCTTTCT CTGGCTGCAT TGTCAGACTG CCTTTGAGCC GCTGTGAGCG ATACGGATCA
    TGCAAAAAGT CTTGTATTGG TGCAAGGGAT CCTTATTGTG GCTGGCTGAA CCATGAGTCC
    TGCGGAAGAG TGAAACCGGG CATGTTTTTT GGGTATGAAC AGGATGTGGA ATACGGCAAC
    ACAGTACAGC TTGGCGACTG TCATGGTGTA CGGTGGGAAG TACAGTCAGG AGACGCCAAC
    CAAATGGTCC ATATGAATGT TTTGATCACC TGCGTTCTTG CTGCTTTTGT GCTCGGAGCT
    TTCATCGCAG GAGTGATAGT TTACTGCTAC AGGGAGATGT TTGTAAGGAA AACAAGAAAA
    ATCCACAAAG ATGCAGAGTC TGCTCAGTCC TGTACAGATT CAAGTGGAAG TTTTGCAAAA
    TTAAATGGAT TGTTTGATAG TCCAGTTAAA GAATATCATC AAAGCATTGA TTCTCCCAAG
    CACTACACAA ATATATTAAC TAGTAGAAAG GAACTGCCAC CAAATGGAGA AACCAAGTCT
    ATGATGATGG ACAGTCAAGG ACAGCCACCT GAACTAGCGG CCCTTCCAAC ACCCGAATCT
    ACCCCAGTTC TTCACCAAAA GAGTTTGCAG CCCATAAAAA ACCAGTGGGA GAAAACACAC
    AATAACTTAA ACATCTCACG GAAAGAAACA CCTCTTAAAA GTCCTCAGTT CTTTCCTTCC
    AGCCCTCCAC CCCATTCACC TTTAGCTCAT GGGCAAATAC CAAGTGCTGT TGTTATTCCA
    AATGCCACCC ATGACTACAA TACTTCCTTT TCAAACTCAA ATGCCCACAA ATCGGATAAA
    AAATTGCATA ACCTGGAGCA CTCTTTGGGA AAACAGTCAA GCAAAAGAGA CCACAGGCGC
    TCTGTTGATT CAAGGAATAC ATTAAATGAC TTTTTGAAGC ATTTGAATGA ATCTGCTGGA
    AACCCCAAAG CTCTTATGGG AGATATTCAA GTAACACATC CGAATGGTCA CCAAGTTCCA
    CATCAAACTT TAATGTTAGA TACAATGGGC AACATGTCTG AAGTTCCACC TAAAGTTCCA
    AACAGAGAGG CATCTCTGTA TTCCCCATCG TCTACTCTAC CAAGAAACAG CCCAACAAAA
    AGAGTAGATG TACCGTCAAC TCCAAATGGG CAAATGACTC CTATAGAATC TCAAAGGAGC
    TATCATAAGA ACTCTTCACA GAGGCATTCA ATATCAGCCA TGCCTAAAAA TATGAACTCA
    CCAAATGGTG TTTTGCTTTC AAGACAGCCA AGTATTAATC GAGGAGGGTA TATGCCTCCA
    AGTCCCAGCA CAAGGATGGA CTATATGCAA GGGACGCCTA TAATAGTGCA CCAACAGACT
    TCTTTGTCAC GGCAAAGTAG TTATACAGGG CATGGAACCC TTCCTCGCAT GGGGGTAAAA
    AGGACACCAT CTATTAAGCC AGATGTGCCA CCAAAACCTT CATTTACTTC ACAAAATACA
    TCTGTAAGAC AGCTGAACAA ATATAGCTAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814019.1
    CDS940..3972
    Translation

    Target ORF information:

    RefSeq Version XM_012958565.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis semaphorin 6D (sema6d), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958565.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGACACTGC GCGCGGGTTG TGCCTGGGCT CTGCTGCTAA CAGTAAGCAC GGTCTGTGCC 
    GTTAGCTTTC CTGAAGACGA CGAGCCTATT AATGTTGTAG ATTATCACTA TTCAAGGCAA
    TATCCAGTAT TTAGAGGACG CCCTTCAGGC AATGATTCAC AGCACAGCCT GGACTTTCAA
    CTGATGCTGA AAATTCGAGA CACACTTTAT GTCGCTGGAA GAGATCAGGT TTATGCTATC
    AATTTAAATG AAGTACCGAA GACTGAAGTA ACACCAAGCA GAAGGTTGAC ATGGAGGTCG
    GGCCAAGCAG ACCGAAAAAA CTGTGCAATG AAAGGAAAGC ACAAGGATGA ATGCCATAAC
    TACATCAAGG TTTTCGTTCC AAGAAATGAT GAGATGGTGT TTGTCTGTGG TACAAATGCA
    TTCAATCCTA TGTGCAGATA TTACAGGTTA AATACTTTTG ATTATGATGG GGAAGAAATT
    AGTGGCTTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTAGCCCT ATTTGCAGAT
    GGCAAGTTAT ATTCTGCTAC CATGGCAGAT TTCCAAGCTA GTGACGCTGT GATCTATCGA
    AGCATGGGGG ATGGATTTGC TCTGAGGACA ATTAAATACG ACTCCAAATG GCTGAAAGAG
    CCACATTTTC TCCATGCAAT AGAATACGGA AATTATATCT ATTTCTTCTT TCGTGAAATT
    GCTGTTGAAC ATAATAATCT AGGCAAGGCC GTACACTCTC GTGTGGCACG CATATGCAAA
    AATGACTTGG GAGGCTCACA GCGAGTGCTG GAGAAACACT GGACTTCATT CGTAAAAGCG
    CGACTCAATT GCTCTGTTCC TGGGGATTCT TTCTTCTACT TTGATGTTCT GCAGTCTATC
    ACGGACATAA TAGAAATCAA TGGAATTCCC ACTGTTGTCG GTGTATTTAC CACACAGCTG
    AACAGCATCC CAGGTTCAGC AGTTTGTGCT TTCACCATGG ATGACATAGA AAAGGTATTC
    AGAGGAAGAT TTAAAGAACA AAAGACCCCA GACTCTGTTT GGACAGCTGT ACCTGAAGAC
    AGAGTACCTA AGCCAAGACC TGGTTGCTGT GCAAGACATG GTTACGGAGA AGGCTACAGG
    TCCTCTACAG AATTCCCTGA CGAAACCTTA TCTTTTATCA AGTCCCATCC ACTGATGGAT
    TCTGCCATCC CATCTATAAT TGAAGAACCT TGGTTCACTA AAACACGCGT AAGGTACAGA
    TTAACTGCCG TAGCAGTGGA CCACTTGGCA GGACCTTACT TAAACTACAC AGTTATGTTT
    GTGGGTTCTG AAGCTGGCGT TGTATTAAAG ATCCTAGCCA AGACAAACCC CTTTTCTCTA
    AATGACAGTG TATTACTGGA AGAAATTGAT GCCTACAACC GTGCAAAGTG TTCTGGAAAT
    AGCGAGGAGG ACAGAAAAGT TATTTCCCTT CAGATGGACA GAGATCATCA TGCCTTGTTT
    GTGGCTTTCT CTGGCTGCAT TGTCAGACTG CCTTTGAGCC GCTGTGAGCG ATACGGATCA
    TGCAAAAAGT CTTGTATTGG TGCAAGGGAT CCTTATTGTG GCTGGCTGAA CCATGAGTCC
    TGCGGAAGAG TGAAACCGGG CATGTTTTTT GGGTATGAAC AGGATGTGGA ATACGGCAAC
    ACAGTACAGC TTGGCGACTG TCATGGTGTA CGGTGGGAAG TACAGTCAGG AGACGCCAAC
    CAAATGGTCC ATATGAATGT TTTGATCACC TGCGTTCTTG CTGCTTTTGT GCTCGGAGCT
    TTCATCGCAG GAGTGATAGT TTACTGCTAC AGGGAGATGT TTGTAAGGAA AACAAGAAAA
    ATCCACAAAG ATGCAGAGTC TGCTCAGTCC TGTACAGATT CAAGTGGAAG TTTTGCAAAA
    TTAAATGGAT TGTTTGATAG TCCAGTTAAA GAATATCATC AAAGCATTGA TTCTCCCAAG
    CACTACACAA ATATATTAAC TAGTAGAAAG GAACTGCCAC CAAATGGAGA AACCAAGTCT
    ATGATGATGG ACAGTCAAGG ACAGCCACCT GAACTAGCGG CCCTTCCAAC ACCCGAATCT
    ACCCCAGTTC TTCACCAAAA GAGTTTGCAG CCCATAAAAA ACCAGTGGGA GAAAACACAC
    AATAACTTAA ACATCTCACG GAAAGAAACA CCTCTTAAAA GTCCTCAGTT CTTTCCTTCC
    AGCCCTCCAC CCCATTCACC TTTAGCTCAT GGGCAAATAC CAAGTGCTGT TGTTATTCCA
    AATGCCACCC ATGACTACAA TACTTCCTTT TCAAACTCAA ATGCCCACAA ATCGGATAAA
    AAATTGCATA ACCTGGAGCA CTCTTTGGGA AAACAGTCAA GCAAAAGAGA CCACAGGCGC
    TCTGTTGATT CAAGGAATAC ATTAAATGAC TTTTTGAAGC ATTTGAATGA ATCTGCTGGA
    AACCCCAAAG CTCTTATGGG AGATATTCAA GTAACACATC CGAATGGTCA CCAAGTTCCA
    CATCAAACTT TAATGTTAGA TACAATGGGC AACATGTCTG AAGTTCCACC TAAAGTTCCA
    AACAGAGAGG CATCTCTGTA TTCCCCATCG TCTACTCTAC CAAGAAACAG CCCAACAAAA
    AGAGTAGATG TACCGTCAAC TCCAAATGGG CAAATGACTC CTATAGAATC TCAAAGGAGC
    TATCATAAGA ACTCTTCACA GAGGCATTCA ATATCAGCCA TGCCTAAAAA TATGAACTCA
    CCAAATGGTG TTTTGCTTTC AAGACAGCCA AGTATTAATC GAGGAGGGTA TATGCCTCCA
    AGTCCCAGCA CAAGGATGGA CTATATGCAA GGGACGCCTA TAATAGTGCA CCAACAGACT
    TCTTTGTCAC GGCAAAGTAG TTATACAGGG CATGGAACCC TTCCTCGCAT GGGGGTAAAA
    AGGACACCAT CTATTAAGCC AGATGTGCCA CCAAAACCTT CATTTACTTC ACAAAATACA
    TCTGTAAGAC AGCTGAACAA ATATAGCTAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa22115
    Clone ID Related Accession (Same CDS sequence) XM_031897617.1 , XM_012958562.3 , NM_001126680.1
    Accession Version NM_001126680.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3255bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Xenopus tropicalis(tropical clawed frog)
    Product semaphorin-6D precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC159147.1. ##Evidence-Data-START## Transcript exon combination :: BC159147.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGACACTGC GCGCGGGTTG TGCCTGGGCT CTGCTGCTAA CAGTAAGCAC GGTCTGTGCC 
    GTTAGCTTTC CTGAAGACGA CGAGCCTATT AATGTTGTAG ATTATCACTA TTCAAGGCAA
    TATCCAGTAT TTAGAGGACG CCCTTCAGGC AATGATTCAC AGCACAGCCT GGACTTTCAA
    CTGATGCTGA AAATTCGAGA CACACTTTAT GTCGCTGGAA GAGATCAGGT TTATGCTATC
    AATTTAAATG AAGTACCGAA GACTGAAGTA ACACCAAGCA GAAGGTTGAC ATGGAGGTCG
    GGCCAAGCAG ACCGAAAAAA CTGTGCAATG AAAGGAAAGC ACAAGGATGA ATGCCATAAC
    TACATCAAGG TTTTCGTTCC AAGAAATGAT GAGATGGTGT TTGTCTGTGG TACAAATGCA
    TTCAATCCTA TGTGCAGATA TTACAGGTTA AATACTTTTG ATTATGATGG GGAAGAAATT
    AGTGGCTTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTAGCCCT ATTTGCAGAT
    GGCAAGTTAT ATTCTGCTAC CATGGCAGAT TTCCAAGCTA GTGACGCTGT GATCTATCGA
    AGCATGGGGG ATGGATTTGC TCTGAGGACA ATTAAATACG ACTCCAAATG GCTGAAAGAG
    CCACATTTTC TCCATGCAAT AGAATACGGA AATTATATCT ATTTCTTCTT TCGTGAAATT
    GCTGTTGAAC ATAATAATCT AGGCAAGGCC GTACACTCTC GTGTGGCACG CATATGCAAA
    AATGACTTGG GAGGCTCACA GCGAGTGCTG GAGAAACACT GGACTTCATT CGTAAAAGCG
    CGACTCAATT GCTCTGTTCC TGGGGATTCT TTCTTCTACT TTGATGTTCT GCAGTCTATC
    ACGGACATAA TAGAAATCAA TGGAATTCCC ACTGTTGTCG GTGTATTTAC CACACAGCTG
    AACAGCATCC CAGGTTCAGC AGTTTGTGCT TTCACCATGG ATGACATAGA AAAGGTATTC
    AGAGGAAGAT TTAAAGAACA AAAGACCCCA GACTCTGTTT GGACAGCTGT ACCTGAAGAC
    AGAGTACCTA AGCCAAGACC TGGTTGCTGT GCAAGACATG GTTACGGAGA AGGCTACAGG
    TCCTCTACAG AATTCCCTGA CGAAACCTTA TCTTTTATCA AGTCCCATCC ACTGATGGAT
    TCTGCCATCC CATCTATAAT TGAAGAACCT TGGTTCACTA AAACACGCGT AAGGTACAGA
    TTAACTGCCG TAGCAGTGGA CCACTTGGCA GGACCTTACT TAAACTACAC AGTTATGTTT
    GTGGGTTCTG AAGCTGGCGT TGTATTAAAG ATCCTAGCCA AGACAAACCC CTTTTCTCTA
    AATGACAGTG TATTACTGGA AGAAATTGAT GCCTACAACC GTGCAAAGTG TTCTGGAAAT
    AGCGAGGAGG ACAGAAAAGT TATTTCCCTT CAGATGGACA GAGATCATCA TGCCTTGTTT
    GTGGCTTTCT CTGGCTGCAT TGTCAGACTG CCTTTGAGCC GCTGTGAGCG ATACGGATCA
    TGCAAAAAGT CTTGTATTGG TGCAAGGGAT CCTTATTGTG GCTGGCTGAA CCATGAGTCC
    TGCGGAAGAG TGAAACCGGG CATGTTTTTT GGGTATGAAC AGGATGTGGA ATACGGCAAC
    ACAGTACAGC TTGGCGACTG TCATGACATT TTGCCTACTG CAACTACACC AGATTACAAA
    ATATTTGGCG ATCCAACATC TGACATTGAA GTTCCTTCTA CTTCTGTAAC CACCACAAGT
    AGCCCAGTTA TTTTACCTAA AGTGATTGGT TCCTGGAAAC CCAGAATGAC TGGCTCACGG
    AAATTTATAT ACCAAGATGA CCCAAACACC TCAGAGTATA TTGATCCGTT AACAGGTGTC
    CAAAAGGGTG TACGGTGGGA AGTACAGTCA GGAGACGCCA ACCAAATGGT CCATATGAAT
    GTTTTGATCA CCTGCGTTCT TGCTGCTTTT GTGCTCGGAG CTTTCATCGC AGGAGTGATA
    GTTTACTGCT ACAGGGAGAT GTTTGTAAGG AAAACAAGAA AAATCCACAA AGATGCAGAG
    TCTGCTCAGT CCTGTACAGA TTCAAGTGGA AGTTTTGCAA AATTAAATGG ATTGTTTGAT
    AGTCCAGTTA AAGAATATCA TCAAAGCATT GATTCTCCCA AGCACTACAC AAATATATTA
    ACTAGTAGAA AGGAACTGCC ACCAAATGGA GAAACCAAGT CTATGATGAT GGACAGTCAA
    GGACAGCCAC CTGAACTAGC GGCCCTTCCA ACACCCGAAT CTACCCCAGT TCTTCACCAA
    AAGAGTTTGC AGCCCATAAA AAACCAGTGG GAGAAAACAC ACAATAACTT AAACATCTCA
    CGGAAAGAAA CACCTCTTAA AAGTCCTCAG TTCTTTCCTT CCAGCCCTCC ACCCCATTCA
    CCTTTAGCTC ATGGGCAAAT ACCAAGTGCT GTTGTTATTC CAAATGCCAC CCATGACTAC
    AATACTTCCT TTTCAAACTC AAATGCCCAC AAATCGGATA AAAAATTGCA TAACCTGGAG
    CACTCTTTGG GAAAACAGTC AAGCAAAAGA GACCACAGGC GCTCTGTTGA TTCAAGGAAT
    ACATTAAATG ACTTTTTGAA GCATTTGAAT GAATCTGCTG GAAACCCCAA AGCTCTTATG
    GGAGATATTC AAGTAACACA TCCGAATGGT CACCAAGTTC CACATCAAAC TTTAATGTTA
    GATACAATGG GCAACATGTC TGAAGTTCCA CCTAAAGTTC CAAACAGAGA GGCATCTCTG
    TATTCCCCAT CGTCTACTCT ACCAAGAAAC AGCCCAACAA AAAGAGTAGA TGTACCGTCA
    ACTCCAAATG GGCAAATGAC TCCTATAGAA TCTCAAAGGA GCTATCATAA GAACTCTTCA
    CAGAGGCATT CAATATCAGC CATGCCTAAA AATATGAACT CACCAAATGG TGTTTTGCTT
    TCAAGACAGC CAAGTATTAA TCGAGGAGGG TATATGCCTC CAAGTCCCAG CACAAGGATG
    GACTATATGC AAGGGACGCC TATAATAGTG CACCAACAGA CTTCTTTGTC ACGGCAAAGT
    AGTTATACAG GGCATGGAAC CCTTCCTCGC ATGGGGGTAA AAAGGACACC ATCTATTAAG
    CCAGATGTGC CACCAAAACC TTCATTTACT TCACAAAATA CATCTGTAAG ACAGCTGAAC
    AAATATAGCT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001120152.1
    CDS598..3852
    Translation

    Target ORF information:

    RefSeq Version NM_001126680.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis semaphorin 6D (sema6d), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001126680.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGACACTGC GCGCGGGTTG TGCCTGGGCT CTGCTGCTAA CAGTAAGCAC GGTCTGTGCC 
    GTTAGCTTTC CTGAAGACGA CGAGCCTATT AATGTTGTAG ATTATCACTA TTCAAGGCAA
    TATCCAGTAT TTAGAGGACG CCCTTCAGGC AATGATTCAC AGCACAGCCT GGACTTTCAA
    CTGATGCTGA AAATTCGAGA CACACTTTAT GTCGCTGGAA GAGATCAGGT TTATGCTATC
    AATTTAAATG AAGTACCGAA GACTGAAGTA ACACCAAGCA GAAGGTTGAC ATGGAGGTCG
    GGCCAAGCAG ACCGAAAAAA CTGTGCAATG AAAGGAAAGC ACAAGGATGA ATGCCATAAC
    TACATCAAGG TTTTCGTTCC AAGAAATGAT GAGATGGTGT TTGTCTGTGG TACAAATGCA
    TTCAATCCTA TGTGCAGATA TTACAGGTTA AATACTTTTG ATTATGATGG GGAAGAAATT
    AGTGGCTTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTAGCCCT ATTTGCAGAT
    GGCAAGTTAT ATTCTGCTAC CATGGCAGAT TTCCAAGCTA GTGACGCTGT GATCTATCGA
    AGCATGGGGG ATGGATTTGC TCTGAGGACA ATTAAATACG ACTCCAAATG GCTGAAAGAG
    CCACATTTTC TCCATGCAAT AGAATACGGA AATTATATCT ATTTCTTCTT TCGTGAAATT
    GCTGTTGAAC ATAATAATCT AGGCAAGGCC GTACACTCTC GTGTGGCACG CATATGCAAA
    AATGACTTGG GAGGCTCACA GCGAGTGCTG GAGAAACACT GGACTTCATT CGTAAAAGCG
    CGACTCAATT GCTCTGTTCC TGGGGATTCT TTCTTCTACT TTGATGTTCT GCAGTCTATC
    ACGGACATAA TAGAAATCAA TGGAATTCCC ACTGTTGTCG GTGTATTTAC CACACAGCTG
    AACAGCATCC CAGGTTCAGC AGTTTGTGCT TTCACCATGG ATGACATAGA AAAGGTATTC
    AGAGGAAGAT TTAAAGAACA AAAGACCCCA GACTCTGTTT GGACAGCTGT ACCTGAAGAC
    AGAGTACCTA AGCCAAGACC TGGTTGCTGT GCAAGACATG GTTACGGAGA AGGCTACAGG
    TCCTCTACAG AATTCCCTGA CGAAACCTTA TCTTTTATCA AGTCCCATCC ACTGATGGAT
    TCTGCCATCC CATCTATAAT TGAAGAACCT TGGTTCACTA AAACACGCGT AAGGTACAGA
    TTAACTGCCG TAGCAGTGGA CCACTTGGCA GGACCTTACT TAAACTACAC AGTTATGTTT
    GTGGGTTCTG AAGCTGGCGT TGTATTAAAG ATCCTAGCCA AGACAAACCC CTTTTCTCTA
    AATGACAGTG TATTACTGGA AGAAATTGAT GCCTACAACC GTGCAAAGTG TTCTGGAAAT
    AGCGAGGAGG ACAGAAAAGT TATTTCCCTT CAGATGGACA GAGATCATCA TGCCTTGTTT
    GTGGCTTTCT CTGGCTGCAT TGTCAGACTG CCTTTGAGCC GCTGTGAGCG ATACGGATCA
    TGCAAAAAGT CTTGTATTGG TGCAAGGGAT CCTTATTGTG GCTGGCTGAA CCATGAGTCC
    TGCGGAAGAG TGAAACCGGG CATGTTTTTT GGGTATGAAC AGGATGTGGA ATACGGCAAC
    ACAGTACAGC TTGGCGACTG TCATGACATT TTGCCTACTG CAACTACACC AGATTACAAA
    ATATTTGGCG ATCCAACATC TGACATTGAA GTTCCTTCTA CTTCTGTAAC CACCACAAGT
    AGCCCAGTTA TTTTACCTAA AGTGATTGGT TCCTGGAAAC CCAGAATGAC TGGCTCACGG
    AAATTTATAT ACCAAGATGA CCCAAACACC TCAGAGTATA TTGATCCGTT AACAGGTGTC
    CAAAAGGGTG TACGGTGGGA AGTACAGTCA GGAGACGCCA ACCAAATGGT CCATATGAAT
    GTTTTGATCA CCTGCGTTCT TGCTGCTTTT GTGCTCGGAG CTTTCATCGC AGGAGTGATA
    GTTTACTGCT ACAGGGAGAT GTTTGTAAGG AAAACAAGAA AAATCCACAA AGATGCAGAG
    TCTGCTCAGT CCTGTACAGA TTCAAGTGGA AGTTTTGCAA AATTAAATGG ATTGTTTGAT
    AGTCCAGTTA AAGAATATCA TCAAAGCATT GATTCTCCCA AGCACTACAC AAATATATTA
    ACTAGTAGAA AGGAACTGCC ACCAAATGGA GAAACCAAGT CTATGATGAT GGACAGTCAA
    GGACAGCCAC CTGAACTAGC GGCCCTTCCA ACACCCGAAT CTACCCCAGT TCTTCACCAA
    AAGAGTTTGC AGCCCATAAA AAACCAGTGG GAGAAAACAC ACAATAACTT AAACATCTCA
    CGGAAAGAAA CACCTCTTAA AAGTCCTCAG TTCTTTCCTT CCAGCCCTCC ACCCCATTCA
    CCTTTAGCTC ATGGGCAAAT ACCAAGTGCT GTTGTTATTC CAAATGCCAC CCATGACTAC
    AATACTTCCT TTTCAAACTC AAATGCCCAC AAATCGGATA AAAAATTGCA TAACCTGGAG
    CACTCTTTGG GAAAACAGTC AAGCAAAAGA GACCACAGGC GCTCTGTTGA TTCAAGGAAT
    ACATTAAATG ACTTTTTGAA GCATTTGAAT GAATCTGCTG GAAACCCCAA AGCTCTTATG
    GGAGATATTC AAGTAACACA TCCGAATGGT CACCAAGTTC CACATCAAAC TTTAATGTTA
    GATACAATGG GCAACATGTC TGAAGTTCCA CCTAAAGTTC CAAACAGAGA GGCATCTCTG
    TATTCCCCAT CGTCTACTCT ACCAAGAAAC AGCCCAACAA AAAGAGTAGA TGTACCGTCA
    ACTCCAAATG GGCAAATGAC TCCTATAGAA TCTCAAAGGA GCTATCATAA GAACTCTTCA
    CAGAGGCATT CAATATCAGC CATGCCTAAA AATATGAACT CACCAAATGG TGTTTTGCTT
    TCAAGACAGC CAAGTATTAA TCGAGGAGGG TATATGCCTC CAAGTCCCAG CACAAGGATG
    GACTATATGC AAGGGACGCC TATAATAGTG CACCAACAGA CTTCTTTGTC ACGGCAAAGT
    AGTTATACAG GGCATGGAAC CCTTCCTCGC ATGGGGGTAA AAAGGACACC ATCTATTAAG
    CCAGATGTGC CACCAAAACC TTCATTTACT TCACAAAATA CATCTGTAAG ACAGCTGAAC
    AAATATAGCT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31618
    Clone ID Related Accession (Same CDS sequence) XM_012958562.2
    Accession Version XM_012958562.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3255bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product semaphorin-6D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958562.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGACACTGC GCGCGGGTTG TGCCTGGGCT CTGCTGCTAA CAGTAAGCAC GGTCTGTGCC 
    GTTAGCTTTC CTGAAGACGA CGAGCCTATT AATGTTGTAG ATTATCACTA TTCAAGGCAA
    TATCCAGTAT TTAGAGGACG CCCTTCAGGC AATGATTCAC AGCACAGCCT GGACTTTCAA
    CTGATGCTGA AAATTCGAGA CACACTTTAT GTCGCTGGAA GAGATCAGGT TTATGCTATC
    AATTTAAATG AAGTACCGAA GACTGAAGTA ACACCAAGCA GAAGGTTGAC ATGGAGGTCG
    GGCCAAGCAG ACCGAAAAAA CTGTGCAATG AAAGGAAAGC ACAAGGATGA ATGCCATAAC
    TACATCAAGG TTTTCGTTCC AAGAAATGAT GAGATGGTGT TTGTCTGTGG TACAAATGCA
    TTCAATCCTA TGTGCAGATA TTACAGGTTA AATACTTTTG ATTATGATGG GGAAGAAATT
    AGTGGCTTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTAGCCCT ATTTGCAGAT
    GGCAAGTTAT ATTCTGCTAC CATGGCAGAT TTCCAAGCTA GTGACGCTGT GATCTATCGA
    AGCATGGGGG ATGGATTTGC TCTGAGGACA ATTAAATACG ACTCCAAATG GCTGAAAGAG
    CCACATTTTC TCCATGCAAT AGAATACGGA AATTATATCT ATTTCTTCTT TCGTGAAATT
    GCTGTTGAAC ATAATAATCT AGGCAAGGCC GTACACTCTC GTGTGGCACG CATATGCAAA
    AATGACTTGG GAGGCTCACA GCGAGTGCTG GAGAAACACT GGACTTCATT CGTAAAAGCG
    CGACTCAATT GCTCTGTTCC TGGGGATTCT TTCTTCTACT TTGATGTTCT GCAGTCTATC
    ACGGACATAA TAGAAATCAA TGGAATTCCC ACTGTTGTCG GTGTATTTAC CACACAGCTG
    AACAGCATCC CAGGTTCAGC AGTTTGTGCT TTCACCATGG ATGACATAGA AAAGGTATTC
    AGAGGAAGAT TTAAAGAACA AAAGACCCCA GACTCTGTTT GGACAGCTGT ACCTGAAGAC
    AGAGTACCTA AGCCAAGACC TGGTTGCTGT GCAAGACATG GTTACGGAGA AGGCTACAGG
    TCCTCTACAG AATTCCCTGA CGAAACCTTA TCTTTTATCA AGTCCCATCC ACTGATGGAT
    TCTGCCATCC CATCTATAAT TGAAGAACCT TGGTTCACTA AAACACGCGT AAGGTACAGA
    TTAACTGCCG TAGCAGTGGA CCACTTGGCA GGACCTTACT TAAACTACAC AGTTATGTTT
    GTGGGTTCTG AAGCTGGCGT TGTATTAAAG ATCCTAGCCA AGACAAACCC CTTTTCTCTA
    AATGACAGTG TATTACTGGA AGAAATTGAT GCCTACAACC GTGCAAAGTG TTCTGGAAAT
    AGCGAGGAGG ACAGAAAAGT TATTTCCCTT CAGATGGACA GAGATCATCA TGCCTTGTTT
    GTGGCTTTCT CTGGCTGCAT TGTCAGACTG CCTTTGAGCC GCTGTGAGCG ATACGGATCA
    TGCAAAAAGT CTTGTATTGG TGCAAGGGAT CCTTATTGTG GCTGGCTGAA CCATGAGTCC
    TGCGGAAGAG TGAAACCGGG CATGTTTTTT GGGTATGAAC AGGATGTGGA ATACGGCAAC
    ACAGTACAGC TTGGCGACTG TCATGACATT TTGCCTACTG CAACTACACC AGATTACAAA
    ATATTTGGCG ATCCAACATC TGACATTGAA GTTCCTTCTA CTTCTGTAAC CACCACAAGT
    AGCCCAGTTA TTTTACCTAA AGTGATTGGT TCCTGGAAAC CCAGAATGAC TGGCTCACGG
    AAATTTATAT ACCAAGATGA CCCAAACACC TCAGAGTATA TTGATCCGTT AACAGGTGTC
    CAAAAGGGTG TACGGTGGGA AGTACAGTCA GGAGACGCCA ACCAAATGGT CCATATGAAT
    GTTTTGATCA CCTGCGTTCT TGCTGCTTTT GTGCTCGGAG CTTTCATCGC AGGAGTGATA
    GTTTACTGCT ACAGGGAGAT GTTTGTAAGG AAAACAAGAA AAATCCACAA AGATGCAGAG
    TCTGCTCAGT CCTGTACAGA TTCAAGTGGA AGTTTTGCAA AATTAAATGG ATTGTTTGAT
    AGTCCAGTTA AAGAATATCA TCAAAGCATT GATTCTCCCA AGCACTACAC AAATATATTG
    ACTAGTAGAA AGGAACTGCC ACCAAATGGA GAAACCAAGT CTATGATGAT GGACAGTCAA
    GGACAGCCAC CTGAACTAGC GGCCCTTCCA ACACCCGAAT CTACCCCAGT TCTTCACCAA
    AAGAGTTTGC AGCCCATAAA AAACCAGTGG GAGAAAACAC ACAATAACTT AAACATCTCA
    CGGAAAGAAA CACCTCTTAA AAGTCCTCAG TTCTTTCCTT CCAGCCCTCC ACCCCATTCA
    CCTTTAGCTC ATGGGCAAAT ACCAAGTGCT GTTGTTATTC CAAATGCCAC CCATGACTAC
    AATACTTCCT TTTCAAACTC AAATGCCCAC AAATCGGATA AAAAATTGCA TAACCTGGAG
    CACTCTTTGG GAAAACAGTC AAGCAAAAGA GACCACAGGC GCTCTGTTGA TTCAAGGAAT
    ACATTAAATG ACTTTTTGAA GCATTTGAAT GAATCTGCTG GAAACCCCAA AGCTCTTATG
    GGAGATATTC AAGTAACACA TCCGAATGGT CACCAAGTTC CACATCAAAC TTTAATGTTA
    GATACAATGG GCAACATGTC TGAAGTTCCA CCTAAAGTTC CAAACAGAGA GGCATCTCTG
    TATTCCCCAT CGTCTACTCT ACCAAGAAAC AGCCCAACAA AAAGAGTAGA TGTACCGTCA
    ACTCCAAATG GGCAAATGAC TCCTATAGAA TCTCAAAGGA GCTATCATAA GAACTCTTCA
    CAGAGGCATT CAATATCAGC CATGCCTAAA AATATGAACT CACCAAATGG TGTTTTGCTT
    TCAAGACAGC CAAGTATTAA TCGAGGAGGG TATATGCCTC CAAGTCCCAG CACAAGGATG
    GACTATATGC AAGGGACGCC TATAATAGTG CACCAACAGA CTTCTTTGTC ACGGCAAAGT
    AGTTATACAG GGCATGGAAC CCTTCCTCGC ATGGGGGTAA AAAGGACACC ATCTATTAAG
    CCAGATGTGC CACCAAAACC TTCATTTACT TCACAAAATA CATCTGTAAG ACAGCTGAAC
    AAATATAGCT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814016.1
    CDS91..3345
    Translation

    Target ORF information:

    RefSeq Version XM_012958562.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (sema6d), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958562.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGACACTGC GCGCGGGTTG TGCCTGGGCT CTGCTGCTAA CAGTAAGCAC GGTCTGTGCC 
    GTTAGCTTTC CTGAAGACGA CGAGCCTATT AATGTTGTAG ATTATCACTA TTCAAGGCAA
    TATCCAGTAT TTAGAGGACG CCCTTCAGGC AATGATTCAC AGCACAGCCT GGACTTTCAA
    CTGATGCTGA AAATTCGAGA CACACTTTAT GTCGCTGGAA GAGATCAGGT TTATGCTATC
    AATTTAAATG AAGTACCGAA GACTGAAGTA ACACCAAGCA GAAGGTTGAC ATGGAGGTCG
    GGCCAAGCAG ACCGAAAAAA CTGTGCAATG AAAGGAAAGC ACAAGGATGA ATGCCATAAC
    TACATCAAGG TTTTCGTTCC AAGAAATGAT GAGATGGTGT TTGTCTGTGG TACAAATGCA
    TTCAATCCTA TGTGCAGATA TTACAGGTTA AATACTTTTG ATTATGATGG GGAAGAAATT
    AGTGGCTTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTAGCCCT ATTTGCAGAT
    GGCAAGTTAT ATTCTGCTAC CATGGCAGAT TTCCAAGCTA GTGACGCTGT GATCTATCGA
    AGCATGGGGG ATGGATTTGC TCTGAGGACA ATTAAATACG ACTCCAAATG GCTGAAAGAG
    CCACATTTTC TCCATGCAAT AGAATACGGA AATTATATCT ATTTCTTCTT TCGTGAAATT
    GCTGTTGAAC ATAATAATCT AGGCAAGGCC GTACACTCTC GTGTGGCACG CATATGCAAA
    AATGACTTGG GAGGCTCACA GCGAGTGCTG GAGAAACACT GGACTTCATT CGTAAAAGCG
    CGACTCAATT GCTCTGTTCC TGGGGATTCT TTCTTCTACT TTGATGTTCT GCAGTCTATC
    ACGGACATAA TAGAAATCAA TGGAATTCCC ACTGTTGTCG GTGTATTTAC CACACAGCTG
    AACAGCATCC CAGGTTCAGC AGTTTGTGCT TTCACCATGG ATGACATAGA AAAGGTATTC
    AGAGGAAGAT TTAAAGAACA AAAGACCCCA GACTCTGTTT GGACAGCTGT ACCTGAAGAC
    AGAGTACCTA AGCCAAGACC TGGTTGCTGT GCAAGACATG GTTACGGAGA AGGCTACAGG
    TCCTCTACAG AATTCCCTGA CGAAACCTTA TCTTTTATCA AGTCCCATCC ACTGATGGAT
    TCTGCCATCC CATCTATAAT TGAAGAACCT TGGTTCACTA AAACACGCGT AAGGTACAGA
    TTAACTGCCG TAGCAGTGGA CCACTTGGCA GGACCTTACT TAAACTACAC AGTTATGTTT
    GTGGGTTCTG AAGCTGGCGT TGTATTAAAG ATCCTAGCCA AGACAAACCC CTTTTCTCTA
    AATGACAGTG TATTACTGGA AGAAATTGAT GCCTACAACC GTGCAAAGTG TTCTGGAAAT
    AGCGAGGAGG ACAGAAAAGT TATTTCCCTT CAGATGGACA GAGATCATCA TGCCTTGTTT
    GTGGCTTTCT CTGGCTGCAT TGTCAGACTG CCTTTGAGCC GCTGTGAGCG ATACGGATCA
    TGCAAAAAGT CTTGTATTGG TGCAAGGGAT CCTTATTGTG GCTGGCTGAA CCATGAGTCC
    TGCGGAAGAG TGAAACCGGG CATGTTTTTT GGGTATGAAC AGGATGTGGA ATACGGCAAC
    ACAGTACAGC TTGGCGACTG TCATGACATT TTGCCTACTG CAACTACACC AGATTACAAA
    ATATTTGGCG ATCCAACATC TGACATTGAA GTTCCTTCTA CTTCTGTAAC CACCACAAGT
    AGCCCAGTTA TTTTACCTAA AGTGATTGGT TCCTGGAAAC CCAGAATGAC TGGCTCACGG
    AAATTTATAT ACCAAGATGA CCCAAACACC TCAGAGTATA TTGATCCGTT AACAGGTGTC
    CAAAAGGGTG TACGGTGGGA AGTACAGTCA GGAGACGCCA ACCAAATGGT CCATATGAAT
    GTTTTGATCA CCTGCGTTCT TGCTGCTTTT GTGCTCGGAG CTTTCATCGC AGGAGTGATA
    GTTTACTGCT ACAGGGAGAT GTTTGTAAGG AAAACAAGAA AAATCCACAA AGATGCAGAG
    TCTGCTCAGT CCTGTACAGA TTCAAGTGGA AGTTTTGCAA AATTAAATGG ATTGTTTGAT
    AGTCCAGTTA AAGAATATCA TCAAAGCATT GATTCTCCCA AGCACTACAC AAATATATTG
    ACTAGTAGAA AGGAACTGCC ACCAAATGGA GAAACCAAGT CTATGATGAT GGACAGTCAA
    GGACAGCCAC CTGAACTAGC GGCCCTTCCA ACACCCGAAT CTACCCCAGT TCTTCACCAA
    AAGAGTTTGC AGCCCATAAA AAACCAGTGG GAGAAAACAC ACAATAACTT AAACATCTCA
    CGGAAAGAAA CACCTCTTAA AAGTCCTCAG TTCTTTCCTT CCAGCCCTCC ACCCCATTCA
    CCTTTAGCTC ATGGGCAAAT ACCAAGTGCT GTTGTTATTC CAAATGCCAC CCATGACTAC
    AATACTTCCT TTTCAAACTC AAATGCCCAC AAATCGGATA AAAAATTGCA TAACCTGGAG
    CACTCTTTGG GAAAACAGTC AAGCAAAAGA GACCACAGGC GCTCTGTTGA TTCAAGGAAT
    ACATTAAATG ACTTTTTGAA GCATTTGAAT GAATCTGCTG GAAACCCCAA AGCTCTTATG
    GGAGATATTC AAGTAACACA TCCGAATGGT CACCAAGTTC CACATCAAAC TTTAATGTTA
    GATACAATGG GCAACATGTC TGAAGTTCCA CCTAAAGTTC CAAACAGAGA GGCATCTCTG
    TATTCCCCAT CGTCTACTCT ACCAAGAAAC AGCCCAACAA AAAGAGTAGA TGTACCGTCA
    ACTCCAAATG GGCAAATGAC TCCTATAGAA TCTCAAAGGA GCTATCATAA GAACTCTTCA
    CAGAGGCATT CAATATCAGC CATGCCTAAA AATATGAACT CACCAAATGG TGTTTTGCTT
    TCAAGACAGC CAAGTATTAA TCGAGGAGGG TATATGCCTC CAAGTCCCAG CACAAGGATG
    GACTATATGC AAGGGACGCC TATAATAGTG CACCAACAGA CTTCTTTGTC ACGGCAAAGT
    AGTTATACAG GGCATGGAAC CCTTCCTCGC ATGGGGGTAA AAAGGACACC ATCTATTAAG
    CCAGATGTGC CACCAAAACC TTCATTTACT TCACAAAATA CATCTGTAAG ACAGCTGAAC
    AAATATAGCT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31619
    Clone ID Related Accession (Same CDS sequence) XM_012958563.2
    Accession Version XM_012958563.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product semaphorin-6D isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958563.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACACTGC GCGCGGGTTG TGCCTGGGCT CTGCTGCTAA CAGTAAGCAC GGTCTGTGCC 
    GTTAGCTTTC CTGAAGACGA CGAGCCTATT AATGTTGTAG ATTATCACTA TTCAAGGCAA
    TATCCAGTAT TTAGAGGACG CCCTTCAGGC AATGATTCAC AGCACAGCCT GGACTTTCAA
    CTGATGCTGA AAATTCGAGA CACACTTTAT GTCGCTGGAA GAGATCAGGT TTATGCTATC
    AATTTAAATG AAGTACCGAA GACTGAAGTA ACACCAAGCA GAAGGTTGAC ATGGAGGTCG
    GGCCAAGCAG ACCGAAAAAA CTGTGCAATG AAAGGAAAGC ACAAGGATGA ATGCCATAAC
    TACATCAAGG TTTTCGTTCC AAGAAATGAT GAGATGGTGT TTGTCTGTGG TACAAATGCA
    TTCAATCCTA TGTGCAGATA TTACAGGTTA AATACTTTTG ATTATGATGG GGAAGAAATT
    AGTGGCTTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTAGCCCT ATTTGCAGAT
    GGCAAGTTAT ATTCTGCTAC CATGGCAGAT TTCCAAGCTA GTGACGCTGT GATCTATCGA
    AGCATGGGGG ATGGATTTGC TCTGAGGACA ATTAAATACG ACTCCAAATG GCTGAAAGAG
    CCACATTTTC TCCATGCAAT AGAATACGGA AATTATATCT ATTTCTTCTT TCGTGAAATT
    GCTGTTGAAC ATAATAATCT AGGCAAGGCC GTACACTCTC GTGTGGCACG CATATGCAAA
    AATGACTTGG GAGGCTCACA GCGAGTGCTG GAGAAACACT GGACTTCATT CGTAAAAGCG
    CGACTCAATT GCTCTGTTCC TGGGGATTCT TTCTTCTACT TTGATGTTCT GCAGTCTATC
    ACGGACATAA TAGAAATCAA TGGAATTCCC ACTGTTGTCG GTGTATTTAC CACACAGCTG
    AACAGCATCC CAGGTTCAGC AGTTTGTGCT TTCACCATGG ATGACATAGA AAAGGTATTC
    AGAGGAAGAT TTAAAGAACA AAAGACCCCA GACTCTGTTT GGACAGCTGT ACCTGAAGAC
    AGAGTACCTA AGCCAAGACC TGGTTGCTGT GCAAGACATG GTTACGGAGA AGGCTACAGG
    TCCTCTACAG AATTCCCTGA CGAAACCTTA TCTTTTATCA AGTCCCATCC ACTGATGGAT
    TCTGCCATCC CATCTATAAT TGAAGAACCT TGGTTCACTA AAACACGCGT AAGGTACAGA
    TTAACTGCCG TAGCAGTGGA CCACTTGGCA GGACCTTACT TAAACTACAC AGTTATGTTT
    GTGGGTTCTG AAGCTGGCGT TGTATTAAAG ATCCTAGCCA AGACAAACCC CTTTTCTCTA
    AATGACAGTG TATTACTGGA AGAAATTGAT GCCTACAACC GTGCAAAGTG TTCTGGAAAT
    AGCGAGGAGG ACAGAAAAGT TATTTCCCTT CAGATGGACA GAGATCATCA TGCCTTGTTT
    GTGGCTTTCT CTGGCTGCAT TGTCAGACTG CCTTTGAGCC GCTGTGAGCG ATACGGATCA
    TGCAAAAAGT CTTGTATTGG TGCAAGGGAT CCTTATTGTG GCTGGCTGAA CCATGAGTCC
    TGCGGAAGAG TGAAACCGGG CATGTTTTTT GGGTATGAAC AGGATGTGGA ATACGGCAAC
    ACAGTACAGC TTGGCGACTG TCATGACATT GAAGTTCCTT CTACTTCTGT AACCACCACA
    AGTAGCCCAG TTATTTTACC TAAAGTGATT GGTTCCTGGA AACCCAGAAT GACTGGCTCA
    CGGAAATTTA TATACCAAGA TGACCCAAAC ACCTCAGAGT ATATTGATCC GTTAACAGGT
    GTCCAAAAGG GTGTACGGTG GGAAGTACAG TCAGGAGACG CCAACCAAAT GGTCCATATG
    AATGTTTTGA TCACCTGCGT TCTTGCTGCT TTTGTGCTCG GAGCTTTCAT CGCAGGAGTG
    ATAGTTTACT GCTACAGGGA GATGTTTGTA AGGAAAACAA GAAAAATCCA CAAAGATGCA
    GAGTCTGCTC AGTCCTGTAC AGATTCAAGT GGAAGTTTTG CAAAATTAAA TGGATTGTTT
    GATAGTCCAG TTAAAGAATA TCATCAAAGC ATTGATTCTC CCAAGCACTA CACAAATATA
    TTGACTAGTA GAAAGGAACT GCCACCAAAT GGAGAAACCA AGTCTATGAT GATGGACAGT
    CAAGGACAGC CACCTGAACT AGCGGCCCTT CCAACACCCG AATCTACCCC AGTTCTTCAC
    CAAAAGAGTT TGCAGCCCAT AAAAAACCAG TGGGAGAAAA CACACAATAA CTTAAACATC
    TCACGGAAAG AAACACCTCT TAAAAGTCCT CAGTTCTTTC CTTCCAGCCC TCCACCCCAT
    TCACCTTTAG CTCATGGGCA AATACCAAGT GCTGTTGTTA TTCCAAATGC CACCCATGAC
    TACAATACTT CCTTTTCAAA CTCAAATGCC CACAAATCGG ATAAAAAATT GCATAACCTG
    GAGCACTCTT TGGGAAAACA GTCAAGCAAA AGAGACCACA GGCGCTCTGT TGATTCAAGG
    AATACATTAA ATGACTTTTT GAAGCATTTG AATGAATCTG CTGGAAACCC CAAAGCTCTT
    ATGGGAGATA TTCAAGTAAC ACATCCGAAT GGTCACCAAG TTCCACATCA AACTTTAATG
    TTAGATACAA TGGGCAACAT GTCTGAAGTT CCACCTAAAG TTCCAAACAG AGAGGCATCT
    CTGTATTCCC CATCGTCTAC TCTACCAAGA AACAGCCCAA CAAAAAGAGT AGATGTACCG
    TCAACTCCAA ATGGGCAAAT GACTCCTATA GAATCTCAAA GGAGCTATCA TAAGAACTCT
    TCACAGAGGC ATTCAATATC AGCCATGCCT AAAAATATGA ACTCACCAAA TGGTGTTTTG
    CTTTCAAGAC AGCCAAGTAT TAATCGAGGA GGGTATATGC CTCCAAGTCC CAGCACAAGG
    ATGGACTATA TGCAAGGGAC GCCTATAATA GTGCACCAAC AGACTTCTTT GTCACGGCAA
    AGTAGTTATA CAGGGCATGG AACCCTTCCT CGCATGGGGG TAAAAAGGAC ACCATCTATT
    AAGCCAGATG TGCCACCAAA ACCTTCATTT ACTTCACAAA ATACATCTGT AAGACAGCTG
    AACAAATATA GCTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814017.1
    CDS1629..4826
    Translation

    Target ORF information:

    RefSeq Version XM_012958563.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (sema6d), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958563.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACACTGC GCGCGGGTTG TGCCTGGGCT CTGCTGCTAA CAGTAAGCAC GGTCTGTGCC 
    GTTAGCTTTC CTGAAGACGA CGAGCCTATT AATGTTGTAG ATTATCACTA TTCAAGGCAA
    TATCCAGTAT TTAGAGGACG CCCTTCAGGC AATGATTCAC AGCACAGCCT GGACTTTCAA
    CTGATGCTGA AAATTCGAGA CACACTTTAT GTCGCTGGAA GAGATCAGGT TTATGCTATC
    AATTTAAATG AAGTACCGAA GACTGAAGTA ACACCAAGCA GAAGGTTGAC ATGGAGGTCG
    GGCCAAGCAG ACCGAAAAAA CTGTGCAATG AAAGGAAAGC ACAAGGATGA ATGCCATAAC
    TACATCAAGG TTTTCGTTCC AAGAAATGAT GAGATGGTGT TTGTCTGTGG TACAAATGCA
    TTCAATCCTA TGTGCAGATA TTACAGGTTA AATACTTTTG ATTATGATGG GGAAGAAATT
    AGTGGCTTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTAGCCCT ATTTGCAGAT
    GGCAAGTTAT ATTCTGCTAC CATGGCAGAT TTCCAAGCTA GTGACGCTGT GATCTATCGA
    AGCATGGGGG ATGGATTTGC TCTGAGGACA ATTAAATACG ACTCCAAATG GCTGAAAGAG
    CCACATTTTC TCCATGCAAT AGAATACGGA AATTATATCT ATTTCTTCTT TCGTGAAATT
    GCTGTTGAAC ATAATAATCT AGGCAAGGCC GTACACTCTC GTGTGGCACG CATATGCAAA
    AATGACTTGG GAGGCTCACA GCGAGTGCTG GAGAAACACT GGACTTCATT CGTAAAAGCG
    CGACTCAATT GCTCTGTTCC TGGGGATTCT TTCTTCTACT TTGATGTTCT GCAGTCTATC
    ACGGACATAA TAGAAATCAA TGGAATTCCC ACTGTTGTCG GTGTATTTAC CACACAGCTG
    AACAGCATCC CAGGTTCAGC AGTTTGTGCT TTCACCATGG ATGACATAGA AAAGGTATTC
    AGAGGAAGAT TTAAAGAACA AAAGACCCCA GACTCTGTTT GGACAGCTGT ACCTGAAGAC
    AGAGTACCTA AGCCAAGACC TGGTTGCTGT GCAAGACATG GTTACGGAGA AGGCTACAGG
    TCCTCTACAG AATTCCCTGA CGAAACCTTA TCTTTTATCA AGTCCCATCC ACTGATGGAT
    TCTGCCATCC CATCTATAAT TGAAGAACCT TGGTTCACTA AAACACGCGT AAGGTACAGA
    TTAACTGCCG TAGCAGTGGA CCACTTGGCA GGACCTTACT TAAACTACAC AGTTATGTTT
    GTGGGTTCTG AAGCTGGCGT TGTATTAAAG ATCCTAGCCA AGACAAACCC CTTTTCTCTA
    AATGACAGTG TATTACTGGA AGAAATTGAT GCCTACAACC GTGCAAAGTG TTCTGGAAAT
    AGCGAGGAGG ACAGAAAAGT TATTTCCCTT CAGATGGACA GAGATCATCA TGCCTTGTTT
    GTGGCTTTCT CTGGCTGCAT TGTCAGACTG CCTTTGAGCC GCTGTGAGCG ATACGGATCA
    TGCAAAAAGT CTTGTATTGG TGCAAGGGAT CCTTATTGTG GCTGGCTGAA CCATGAGTCC
    TGCGGAAGAG TGAAACCGGG CATGTTTTTT GGGTATGAAC AGGATGTGGA ATACGGCAAC
    ACAGTACAGC TTGGCGACTG TCATGACATT GAAGTTCCTT CTACTTCTGT AACCACCACA
    AGTAGCCCAG TTATTTTACC TAAAGTGATT GGTTCCTGGA AACCCAGAAT GACTGGCTCA
    CGGAAATTTA TATACCAAGA TGACCCAAAC ACCTCAGAGT ATATTGATCC GTTAACAGGT
    GTCCAAAAGG GTGTACGGTG GGAAGTACAG TCAGGAGACG CCAACCAAAT GGTCCATATG
    AATGTTTTGA TCACCTGCGT TCTTGCTGCT TTTGTGCTCG GAGCTTTCAT CGCAGGAGTG
    ATAGTTTACT GCTACAGGGA GATGTTTGTA AGGAAAACAA GAAAAATCCA CAAAGATGCA
    GAGTCTGCTC AGTCCTGTAC AGATTCAAGT GGAAGTTTTG CAAAATTAAA TGGATTGTTT
    GATAGTCCAG TTAAAGAATA TCATCAAAGC ATTGATTCTC CCAAGCACTA CACAAATATA
    TTGACTAGTA GAAAGGAACT GCCACCAAAT GGAGAAACCA AGTCTATGAT GATGGACAGT
    CAAGGACAGC CACCTGAACT AGCGGCCCTT CCAACACCCG AATCTACCCC AGTTCTTCAC
    CAAAAGAGTT TGCAGCCCAT AAAAAACCAG TGGGAGAAAA CACACAATAA CTTAAACATC
    TCACGGAAAG AAACACCTCT TAAAAGTCCT CAGTTCTTTC CTTCCAGCCC TCCACCCCAT
    TCACCTTTAG CTCATGGGCA AATACCAAGT GCTGTTGTTA TTCCAAATGC CACCCATGAC
    TACAATACTT CCTTTTCAAA CTCAAATGCC CACAAATCGG ATAAAAAATT GCATAACCTG
    GAGCACTCTT TGGGAAAACA GTCAAGCAAA AGAGACCACA GGCGCTCTGT TGATTCAAGG
    AATACATTAA ATGACTTTTT GAAGCATTTG AATGAATCTG CTGGAAACCC CAAAGCTCTT
    ATGGGAGATA TTCAAGTAAC ACATCCGAAT GGTCACCAAG TTCCACATCA AACTTTAATG
    TTAGATACAA TGGGCAACAT GTCTGAAGTT CCACCTAAAG TTCCAAACAG AGAGGCATCT
    CTGTATTCCC CATCGTCTAC TCTACCAAGA AACAGCCCAA CAAAAAGAGT AGATGTACCG
    TCAACTCCAA ATGGGCAAAT GACTCCTATA GAATCTCAAA GGAGCTATCA TAAGAACTCT
    TCACAGAGGC ATTCAATATC AGCCATGCCT AAAAATATGA ACTCACCAAA TGGTGTTTTG
    CTTTCAAGAC AGCCAAGTAT TAATCGAGGA GGGTATATGC CTCCAAGTCC CAGCACAAGG
    ATGGACTATA TGCAAGGGAC GCCTATAATA GTGCACCAAC AGACTTCTTT GTCACGGCAA
    AGTAGTTATA CAGGGCATGG AACCCTTCCT CGCATGGGGG TAAAAAGGAC ACCATCTATT
    AAGCCAGATG TGCCACCAAA ACCTTCATTT ACTTCACAAA ATACATCTGT AAGACAGCTG
    AACAAATATA GCTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31620
    Clone ID Related Accession (Same CDS sequence) XM_012958564.2
    Accession Version XM_012958564.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3090bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product semaphorin-6D isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958564.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGACACTGC GCGCGGGTTG TGCCTGGGCT CTGCTGCTAA CAGTAAGCAC GGTCTGTGCC 
    GTTAGCTTTC CTGAAGACGA CGAGCCTATT AATGTTGTAG ATTATCACTA TTCAAGGCAA
    TATCCAGTAT TTAGAGGACG CCCTTCAGGC AATGATTCAC AGCACAGCCT GGACTTTCAA
    CTGATGCTGA AAATTCGAGA CACACTTTAT GTCGCTGGAA GAGATCAGGT TTATGCTATC
    AATTTAAATG AAGTACCGAA GACTGAAGTA ACACCAAGCA GAAGGTTGAC ATGGAGGTCG
    GGCCAAGCAG ACCGAAAAAA CTGTGCAATG AAAGGAAAGC ACAAGGATGA ATGCCATAAC
    TACATCAAGG TTTTCGTTCC AAGAAATGAT GAGATGGTGT TTGTCTGTGG TACAAATGCA
    TTCAATCCTA TGTGCAGATA TTACAGGTTA AATACTTTTG ATTATGATGG GGAAGAAATT
    AGTGGCTTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTAGCCCT ATTTGCAGAT
    GGCAAGTTAT ATTCTGCTAC CATGGCAGAT TTCCAAGCTA GTGACGCTGT GATCTATCGA
    AGCATGGGGG ATGGATTTGC TCTGAGGACA ATTAAATACG ACTCCAAATG GCTGAAAGAG
    CCACATTTTC TCCATGCAAT AGAATACGGA AATTATATCT ATTTCTTCTT TCGTGAAATT
    GCTGTTGAAC ATAATAATCT AGGCAAGGCC GTACACTCTC GTGTGGCACG CATATGCAAA
    AATGACTTGG GAGGCTCACA GCGAGTGCTG GAGAAACACT GGACTTCATT CGTAAAAGCG
    CGACTCAATT GCTCTGTTCC TGGGGATTCT TTCTTCTACT TTGATGTTCT GCAGTCTATC
    ACGGACATAA TAGAAATCAA TGGAATTCCC ACTGTTGTCG GTGTATTTAC CACACAGCTG
    AACAGCATCC CAGGTTCAGC AGTTTGTGCT TTCACCATGG ATGACATAGA AAAGGTATTC
    AGAGGAAGAT TTAAAGAACA AAAGACCCCA GACTCTGTTT GGACAGCTGT ACCTGAAGAC
    AGAGTACCTA AGCCAAGACC TGGTTGCTGT GCAAGACATG GTTACGGAGA AGGCTACAGG
    TCCTCTACAG AATTCCCTGA CGAAACCTTA TCTTTTATCA AGTCCCATCC ACTGATGGAT
    TCTGCCATCC CATCTATAAT TGAAGAACCT TGGTTCACTA AAACACGCGT AAGGTACAGA
    TTAACTGCCG TAGCAGTGGA CCACTTGGCA GGACCTTACT TAAACTACAC AGTTATGTTT
    GTGGGTTCTG AAGCTGGCGT TGTATTAAAG ATCCTAGCCA AGACAAACCC CTTTTCTCTA
    AATGACAGTG TATTACTGGA AGAAATTGAT GCCTACAACC GTGCAAAGTG TTCTGGAAAT
    AGCGAGGAGG ACAGAAAAGT TATTTCCCTT CAGATGGACA GAGATCATCA TGCCTTGTTT
    GTGGCTTTCT CTGGCTGCAT TGTCAGACTG CCTTTGAGCC GCTGTGAGCG ATACGGATCA
    TGCAAAAAGT CTTGTATTGG TGCAAGGGAT CCTTATTGTG GCTGGCTGAA CCATGAGTCC
    TGCGGAAGAG TGAAACCGGG CATGTTTTTT GGGTATGAAC AGGATGTGGA ATACGGCAAC
    ACAGTACAGC TTGGCGACTG TCATGACATT TTGCCTACTG CAACTACACC AGATTACAAA
    ATATTTGGCG ATCCAACATC TGGTGTACGG TGGGAAGTAC AGTCAGGAGA CGCCAACCAA
    ATGGTCCATA TGAATGTTTT GATCACCTGC GTTCTTGCTG CTTTTGTGCT CGGAGCTTTC
    ATCGCAGGAG TGATAGTTTA CTGCTACAGG GAGATGTTTG TAAGGAAAAC AAGAAAAATC
    CACAAAGATG CAGAGTCTGC TCAGTCCTGT ACAGATTCAA GTGGAAGTTT TGCAAAATTA
    AATGGATTGT TTGATAGTCC AGTTAAAGAA TATCATCAAA GCATTGATTC TCCCAAGCAC
    TACACAAATA TATTGACTAG TAGAAAGGAA CTGCCACCAA ATGGAGAAAC CAAGTCTATG
    ATGATGGACA GTCAAGGACA GCCACCTGAA CTAGCGGCCC TTCCAACACC CGAATCTACC
    CCAGTTCTTC ACCAAAAGAG TTTGCAGCCC ATAAAAAACC AGTGGGAGAA AACACACAAT
    AACTTAAACA TCTCACGGAA AGAAACACCT CTTAAAAGTC CTCAGTTCTT TCCTTCCAGC
    CCTCCACCCC ATTCACCTTT AGCTCATGGG CAAATACCAA GTGCTGTTGT TATTCCAAAT
    GCCACCCATG ACTACAATAC TTCCTTTTCA AACTCAAATG CCCACAAATC GGATAAAAAA
    TTGCATAACC TGGAGCACTC TTTGGGAAAA CAGTCAAGCA AAAGAGACCA CAGGCGCTCT
    GTTGATTCAA GGAATACATT AAATGACTTT TTGAAGCATT TGAATGAATC TGCTGGAAAC
    CCCAAAGCTC TTATGGGAGA TATTCAAGTA ACACATCCGA ATGGTCACCA AGTTCCACAT
    CAAACTTTAA TGTTAGATAC AATGGGCAAC ATGTCTGAAG TTCCACCTAA AGTTCCAAAC
    AGAGAGGCAT CTCTGTATTC CCCATCGTCT ACTCTACCAA GAAACAGCCC AACAAAAAGA
    GTAGATGTAC CGTCAACTCC AAATGGGCAA ATGACTCCTA TAGAATCTCA AAGGAGCTAT
    CATAAGAACT CTTCACAGAG GCATTCAATA TCAGCCATGC CTAAAAATAT GAACTCACCA
    AATGGTGTTT TGCTTTCAAG ACAGCCAAGT ATTAATCGAG GAGGGTATAT GCCTCCAAGT
    CCCAGCACAA GGATGGACTA TATGCAAGGG ACGCCTATAA TAGTGCACCA ACAGACTTCT
    TTGTCACGGC AAAGTAGTTA TACAGGGCAT GGAACCCTTC CTCGCATGGG GGTAAAAAGG
    ACACCATCTA TTAAGCCAGA TGTGCCACCA AAACCTTCAT TTACTTCACA AAATACATCT
    GTAAGACAGC TGAACAAATA TAGCTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814018.1
    CDS1628..4717
    Translation

    Target ORF information:

    RefSeq Version XM_012958564.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (sema6d), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958564.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGACACTGC GCGCGGGTTG TGCCTGGGCT CTGCTGCTAA CAGTAAGCAC GGTCTGTGCC 
    GTTAGCTTTC CTGAAGACGA CGAGCCTATT AATGTTGTAG ATTATCACTA TTCAAGGCAA
    TATCCAGTAT TTAGAGGACG CCCTTCAGGC AATGATTCAC AGCACAGCCT GGACTTTCAA
    CTGATGCTGA AAATTCGAGA CACACTTTAT GTCGCTGGAA GAGATCAGGT TTATGCTATC
    AATTTAAATG AAGTACCGAA GACTGAAGTA ACACCAAGCA GAAGGTTGAC ATGGAGGTCG
    GGCCAAGCAG ACCGAAAAAA CTGTGCAATG AAAGGAAAGC ACAAGGATGA ATGCCATAAC
    TACATCAAGG TTTTCGTTCC AAGAAATGAT GAGATGGTGT TTGTCTGTGG TACAAATGCA
    TTCAATCCTA TGTGCAGATA TTACAGGTTA AATACTTTTG ATTATGATGG GGAAGAAATT
    AGTGGCTTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTAGCCCT ATTTGCAGAT
    GGCAAGTTAT ATTCTGCTAC CATGGCAGAT TTCCAAGCTA GTGACGCTGT GATCTATCGA
    AGCATGGGGG ATGGATTTGC TCTGAGGACA ATTAAATACG ACTCCAAATG GCTGAAAGAG
    CCACATTTTC TCCATGCAAT AGAATACGGA AATTATATCT ATTTCTTCTT TCGTGAAATT
    GCTGTTGAAC ATAATAATCT AGGCAAGGCC GTACACTCTC GTGTGGCACG CATATGCAAA
    AATGACTTGG GAGGCTCACA GCGAGTGCTG GAGAAACACT GGACTTCATT CGTAAAAGCG
    CGACTCAATT GCTCTGTTCC TGGGGATTCT TTCTTCTACT TTGATGTTCT GCAGTCTATC
    ACGGACATAA TAGAAATCAA TGGAATTCCC ACTGTTGTCG GTGTATTTAC CACACAGCTG
    AACAGCATCC CAGGTTCAGC AGTTTGTGCT TTCACCATGG ATGACATAGA AAAGGTATTC
    AGAGGAAGAT TTAAAGAACA AAAGACCCCA GACTCTGTTT GGACAGCTGT ACCTGAAGAC
    AGAGTACCTA AGCCAAGACC TGGTTGCTGT GCAAGACATG GTTACGGAGA AGGCTACAGG
    TCCTCTACAG AATTCCCTGA CGAAACCTTA TCTTTTATCA AGTCCCATCC ACTGATGGAT
    TCTGCCATCC CATCTATAAT TGAAGAACCT TGGTTCACTA AAACACGCGT AAGGTACAGA
    TTAACTGCCG TAGCAGTGGA CCACTTGGCA GGACCTTACT TAAACTACAC AGTTATGTTT
    GTGGGTTCTG AAGCTGGCGT TGTATTAAAG ATCCTAGCCA AGACAAACCC CTTTTCTCTA
    AATGACAGTG TATTACTGGA AGAAATTGAT GCCTACAACC GTGCAAAGTG TTCTGGAAAT
    AGCGAGGAGG ACAGAAAAGT TATTTCCCTT CAGATGGACA GAGATCATCA TGCCTTGTTT
    GTGGCTTTCT CTGGCTGCAT TGTCAGACTG CCTTTGAGCC GCTGTGAGCG ATACGGATCA
    TGCAAAAAGT CTTGTATTGG TGCAAGGGAT CCTTATTGTG GCTGGCTGAA CCATGAGTCC
    TGCGGAAGAG TGAAACCGGG CATGTTTTTT GGGTATGAAC AGGATGTGGA ATACGGCAAC
    ACAGTACAGC TTGGCGACTG TCATGACATT TTGCCTACTG CAACTACACC AGATTACAAA
    ATATTTGGCG ATCCAACATC TGGTGTACGG TGGGAAGTAC AGTCAGGAGA CGCCAACCAA
    ATGGTCCATA TGAATGTTTT GATCACCTGC GTTCTTGCTG CTTTTGTGCT CGGAGCTTTC
    ATCGCAGGAG TGATAGTTTA CTGCTACAGG GAGATGTTTG TAAGGAAAAC AAGAAAAATC
    CACAAAGATG CAGAGTCTGC TCAGTCCTGT ACAGATTCAA GTGGAAGTTT TGCAAAATTA
    AATGGATTGT TTGATAGTCC AGTTAAAGAA TATCATCAAA GCATTGATTC TCCCAAGCAC
    TACACAAATA TATTGACTAG TAGAAAGGAA CTGCCACCAA ATGGAGAAAC CAAGTCTATG
    ATGATGGACA GTCAAGGACA GCCACCTGAA CTAGCGGCCC TTCCAACACC CGAATCTACC
    CCAGTTCTTC ACCAAAAGAG TTTGCAGCCC ATAAAAAACC AGTGGGAGAA AACACACAAT
    AACTTAAACA TCTCACGGAA AGAAACACCT CTTAAAAGTC CTCAGTTCTT TCCTTCCAGC
    CCTCCACCCC ATTCACCTTT AGCTCATGGG CAAATACCAA GTGCTGTTGT TATTCCAAAT
    GCCACCCATG ACTACAATAC TTCCTTTTCA AACTCAAATG CCCACAAATC GGATAAAAAA
    TTGCATAACC TGGAGCACTC TTTGGGAAAA CAGTCAAGCA AAAGAGACCA CAGGCGCTCT
    GTTGATTCAA GGAATACATT AAATGACTTT TTGAAGCATT TGAATGAATC TGCTGGAAAC
    CCCAAAGCTC TTATGGGAGA TATTCAAGTA ACACATCCGA ATGGTCACCA AGTTCCACAT
    CAAACTTTAA TGTTAGATAC AATGGGCAAC ATGTCTGAAG TTCCACCTAA AGTTCCAAAC
    AGAGAGGCAT CTCTGTATTC CCCATCGTCT ACTCTACCAA GAAACAGCCC AACAAAAAGA
    GTAGATGTAC CGTCAACTCC AAATGGGCAA ATGACTCCTA TAGAATCTCA AAGGAGCTAT
    CATAAGAACT CTTCACAGAG GCATTCAATA TCAGCCATGC CTAAAAATAT GAACTCACCA
    AATGGTGTTT TGCTTTCAAG ACAGCCAAGT ATTAATCGAG GAGGGTATAT GCCTCCAAGT
    CCCAGCACAA GGATGGACTA TATGCAAGGG ACGCCTATAA TAGTGCACCA ACAGACTTCT
    TTGTCACGGC AAAGTAGTTA TACAGGGCAT GGAACCCTTC CTCGCATGGG GGTAAAAAGG
    ACACCATCTA TTAAGCCAGA TGTGCCACCA AAACCTTCAT TTACTTCACA AAATACATCT
    GTAAGACAGC TGAACAAATA TAGCTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31621
    Clone ID Related Accession (Same CDS sequence) XM_012958565.2
    Accession Version XM_012958565.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3033bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product semaphorin-6D isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958565.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGACACTGC GCGCGGGTTG TGCCTGGGCT CTGCTGCTAA CAGTAAGCAC GGTCTGTGCC 
    GTTAGCTTTC CTGAAGACGA CGAGCCTATT AATGTTGTAG ATTATCACTA TTCAAGGCAA
    TATCCAGTAT TTAGAGGACG CCCTTCAGGC AATGATTCAC AGCACAGCCT GGACTTTCAA
    CTGATGCTGA AAATTCGAGA CACACTTTAT GTCGCTGGAA GAGATCAGGT TTATGCTATC
    AATTTAAATG AAGTACCGAA GACTGAAGTA ACACCAAGCA GAAGGTTGAC ATGGAGGTCG
    GGCCAAGCAG ACCGAAAAAA CTGTGCAATG AAAGGAAAGC ACAAGGATGA ATGCCATAAC
    TACATCAAGG TTTTCGTTCC AAGAAATGAT GAGATGGTGT TTGTCTGTGG TACAAATGCA
    TTCAATCCTA TGTGCAGATA TTACAGGTTA AATACTTTTG ATTATGATGG GGAAGAAATT
    AGTGGCTTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTAGCCCT ATTTGCAGAT
    GGCAAGTTAT ATTCTGCTAC CATGGCAGAT TTCCAAGCTA GTGACGCTGT GATCTATCGA
    AGCATGGGGG ATGGATTTGC TCTGAGGACA ATTAAATACG ACTCCAAATG GCTGAAAGAG
    CCACATTTTC TCCATGCAAT AGAATACGGA AATTATATCT ATTTCTTCTT TCGTGAAATT
    GCTGTTGAAC ATAATAATCT AGGCAAGGCC GTACACTCTC GTGTGGCACG CATATGCAAA
    AATGACTTGG GAGGCTCACA GCGAGTGCTG GAGAAACACT GGACTTCATT CGTAAAAGCG
    CGACTCAATT GCTCTGTTCC TGGGGATTCT TTCTTCTACT TTGATGTTCT GCAGTCTATC
    ACGGACATAA TAGAAATCAA TGGAATTCCC ACTGTTGTCG GTGTATTTAC CACACAGCTG
    AACAGCATCC CAGGTTCAGC AGTTTGTGCT TTCACCATGG ATGACATAGA AAAGGTATTC
    AGAGGAAGAT TTAAAGAACA AAAGACCCCA GACTCTGTTT GGACAGCTGT ACCTGAAGAC
    AGAGTACCTA AGCCAAGACC TGGTTGCTGT GCAAGACATG GTTACGGAGA AGGCTACAGG
    TCCTCTACAG AATTCCCTGA CGAAACCTTA TCTTTTATCA AGTCCCATCC ACTGATGGAT
    TCTGCCATCC CATCTATAAT TGAAGAACCT TGGTTCACTA AAACACGCGT AAGGTACAGA
    TTAACTGCCG TAGCAGTGGA CCACTTGGCA GGACCTTACT TAAACTACAC AGTTATGTTT
    GTGGGTTCTG AAGCTGGCGT TGTATTAAAG ATCCTAGCCA AGACAAACCC CTTTTCTCTA
    AATGACAGTG TATTACTGGA AGAAATTGAT GCCTACAACC GTGCAAAGTG TTCTGGAAAT
    AGCGAGGAGG ACAGAAAAGT TATTTCCCTT CAGATGGACA GAGATCATCA TGCCTTGTTT
    GTGGCTTTCT CTGGCTGCAT TGTCAGACTG CCTTTGAGCC GCTGTGAGCG ATACGGATCA
    TGCAAAAAGT CTTGTATTGG TGCAAGGGAT CCTTATTGTG GCTGGCTGAA CCATGAGTCC
    TGCGGAAGAG TGAAACCGGG CATGTTTTTT GGGTATGAAC AGGATGTGGA ATACGGCAAC
    ACAGTACAGC TTGGCGACTG TCATGGTGTA CGGTGGGAAG TACAGTCAGG AGACGCCAAC
    CAAATGGTCC ATATGAATGT TTTGATCACC TGCGTTCTTG CTGCTTTTGT GCTCGGAGCT
    TTCATCGCAG GAGTGATAGT TTACTGCTAC AGGGAGATGT TTGTAAGGAA AACAAGAAAA
    ATCCACAAAG ATGCAGAGTC TGCTCAGTCC TGTACAGATT CAAGTGGAAG TTTTGCAAAA
    TTAAATGGAT TGTTTGATAG TCCAGTTAAA GAATATCATC AAAGCATTGA TTCTCCCAAG
    CACTACACAA ATATATTGAC TAGTAGAAAG GAACTGCCAC CAAATGGAGA AACCAAGTCT
    ATGATGATGG ACAGTCAAGG ACAGCCACCT GAACTAGCGG CCCTTCCAAC ACCCGAATCT
    ACCCCAGTTC TTCACCAAAA GAGTTTGCAG CCCATAAAAA ACCAGTGGGA GAAAACACAC
    AATAACTTAA ACATCTCACG GAAAGAAACA CCTCTTAAAA GTCCTCAGTT CTTTCCTTCC
    AGCCCTCCAC CCCATTCACC TTTAGCTCAT GGGCAAATAC CAAGTGCTGT TGTTATTCCA
    AATGCCACCC ATGACTACAA TACTTCCTTT TCAAACTCAA ATGCCCACAA ATCGGATAAA
    AAATTGCATA ACCTGGAGCA CTCTTTGGGA AAACAGTCAA GCAAAAGAGA CCACAGGCGC
    TCTGTTGATT CAAGGAATAC ATTAAATGAC TTTTTGAAGC ATTTGAATGA ATCTGCTGGA
    AACCCCAAAG CTCTTATGGG AGATATTCAA GTAACACATC CGAATGGTCA CCAAGTTCCA
    CATCAAACTT TAATGTTAGA TACAATGGGC AACATGTCTG AAGTTCCACC TAAAGTTCCA
    AACAGAGAGG CATCTCTGTA TTCCCCATCG TCTACTCTAC CAAGAAACAG CCCAACAAAA
    AGAGTAGATG TACCGTCAAC TCCAAATGGG CAAATGACTC CTATAGAATC TCAAAGGAGC
    TATCATAAGA ACTCTTCACA GAGGCATTCA ATATCAGCCA TGCCTAAAAA TATGAACTCA
    CCAAATGGTG TTTTGCTTTC AAGACAGCCA AGTATTAATC GAGGAGGGTA TATGCCTCCA
    AGTCCCAGCA CAAGGATGGA CTATATGCAA GGGACGCCTA TAATAGTGCA CCAACAGACT
    TCTTTGTCAC GGCAAAGTAG TTATACAGGG CATGGAACCC TTCCTCGCAT GGGGGTAAAA
    AGGACACCAT CTATTAAGCC AGATGTGCCA CCAAAACCTT CATTTACTTC ACAAAATACA
    TCTGTAAGAC AGCTGAACAA ATATAGCTAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814019.1
    CDS1629..4661
    Translation

    Target ORF information:

    RefSeq Version XM_012958565.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (sema6d), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958565.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGACACTGC GCGCGGGTTG TGCCTGGGCT CTGCTGCTAA CAGTAAGCAC GGTCTGTGCC 
    GTTAGCTTTC CTGAAGACGA CGAGCCTATT AATGTTGTAG ATTATCACTA TTCAAGGCAA
    TATCCAGTAT TTAGAGGACG CCCTTCAGGC AATGATTCAC AGCACAGCCT GGACTTTCAA
    CTGATGCTGA AAATTCGAGA CACACTTTAT GTCGCTGGAA GAGATCAGGT TTATGCTATC
    AATTTAAATG AAGTACCGAA GACTGAAGTA ACACCAAGCA GAAGGTTGAC ATGGAGGTCG
    GGCCAAGCAG ACCGAAAAAA CTGTGCAATG AAAGGAAAGC ACAAGGATGA ATGCCATAAC
    TACATCAAGG TTTTCGTTCC AAGAAATGAT GAGATGGTGT TTGTCTGTGG TACAAATGCA
    TTCAATCCTA TGTGCAGATA TTACAGGTTA AATACTTTTG ATTATGATGG GGAAGAAATT
    AGTGGCTTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTAGCCCT ATTTGCAGAT
    GGCAAGTTAT ATTCTGCTAC CATGGCAGAT TTCCAAGCTA GTGACGCTGT GATCTATCGA
    AGCATGGGGG ATGGATTTGC TCTGAGGACA ATTAAATACG ACTCCAAATG GCTGAAAGAG
    CCACATTTTC TCCATGCAAT AGAATACGGA AATTATATCT ATTTCTTCTT TCGTGAAATT
    GCTGTTGAAC ATAATAATCT AGGCAAGGCC GTACACTCTC GTGTGGCACG CATATGCAAA
    AATGACTTGG GAGGCTCACA GCGAGTGCTG GAGAAACACT GGACTTCATT CGTAAAAGCG
    CGACTCAATT GCTCTGTTCC TGGGGATTCT TTCTTCTACT TTGATGTTCT GCAGTCTATC
    ACGGACATAA TAGAAATCAA TGGAATTCCC ACTGTTGTCG GTGTATTTAC CACACAGCTG
    AACAGCATCC CAGGTTCAGC AGTTTGTGCT TTCACCATGG ATGACATAGA AAAGGTATTC
    AGAGGAAGAT TTAAAGAACA AAAGACCCCA GACTCTGTTT GGACAGCTGT ACCTGAAGAC
    AGAGTACCTA AGCCAAGACC TGGTTGCTGT GCAAGACATG GTTACGGAGA AGGCTACAGG
    TCCTCTACAG AATTCCCTGA CGAAACCTTA TCTTTTATCA AGTCCCATCC ACTGATGGAT
    TCTGCCATCC CATCTATAAT TGAAGAACCT TGGTTCACTA AAACACGCGT AAGGTACAGA
    TTAACTGCCG TAGCAGTGGA CCACTTGGCA GGACCTTACT TAAACTACAC AGTTATGTTT
    GTGGGTTCTG AAGCTGGCGT TGTATTAAAG ATCCTAGCCA AGACAAACCC CTTTTCTCTA
    AATGACAGTG TATTACTGGA AGAAATTGAT GCCTACAACC GTGCAAAGTG TTCTGGAAAT
    AGCGAGGAGG ACAGAAAAGT TATTTCCCTT CAGATGGACA GAGATCATCA TGCCTTGTTT
    GTGGCTTTCT CTGGCTGCAT TGTCAGACTG CCTTTGAGCC GCTGTGAGCG ATACGGATCA
    TGCAAAAAGT CTTGTATTGG TGCAAGGGAT CCTTATTGTG GCTGGCTGAA CCATGAGTCC
    TGCGGAAGAG TGAAACCGGG CATGTTTTTT GGGTATGAAC AGGATGTGGA ATACGGCAAC
    ACAGTACAGC TTGGCGACTG TCATGGTGTA CGGTGGGAAG TACAGTCAGG AGACGCCAAC
    CAAATGGTCC ATATGAATGT TTTGATCACC TGCGTTCTTG CTGCTTTTGT GCTCGGAGCT
    TTCATCGCAG GAGTGATAGT TTACTGCTAC AGGGAGATGT TTGTAAGGAA AACAAGAAAA
    ATCCACAAAG ATGCAGAGTC TGCTCAGTCC TGTACAGATT CAAGTGGAAG TTTTGCAAAA
    TTAAATGGAT TGTTTGATAG TCCAGTTAAA GAATATCATC AAAGCATTGA TTCTCCCAAG
    CACTACACAA ATATATTGAC TAGTAGAAAG GAACTGCCAC CAAATGGAGA AACCAAGTCT
    ATGATGATGG ACAGTCAAGG ACAGCCACCT GAACTAGCGG CCCTTCCAAC ACCCGAATCT
    ACCCCAGTTC TTCACCAAAA GAGTTTGCAG CCCATAAAAA ACCAGTGGGA GAAAACACAC
    AATAACTTAA ACATCTCACG GAAAGAAACA CCTCTTAAAA GTCCTCAGTT CTTTCCTTCC
    AGCCCTCCAC CCCATTCACC TTTAGCTCAT GGGCAAATAC CAAGTGCTGT TGTTATTCCA
    AATGCCACCC ATGACTACAA TACTTCCTTT TCAAACTCAA ATGCCCACAA ATCGGATAAA
    AAATTGCATA ACCTGGAGCA CTCTTTGGGA AAACAGTCAA GCAAAAGAGA CCACAGGCGC
    TCTGTTGATT CAAGGAATAC ATTAAATGAC TTTTTGAAGC ATTTGAATGA ATCTGCTGGA
    AACCCCAAAG CTCTTATGGG AGATATTCAA GTAACACATC CGAATGGTCA CCAAGTTCCA
    CATCAAACTT TAATGTTAGA TACAATGGGC AACATGTCTG AAGTTCCACC TAAAGTTCCA
    AACAGAGAGG CATCTCTGTA TTCCCCATCG TCTACTCTAC CAAGAAACAG CCCAACAAAA
    AGAGTAGATG TACCGTCAAC TCCAAATGGG CAAATGACTC CTATAGAATC TCAAAGGAGC
    TATCATAAGA ACTCTTCACA GAGGCATTCA ATATCAGCCA TGCCTAAAAA TATGAACTCA
    CCAAATGGTG TTTTGCTTTC AAGACAGCCA AGTATTAATC GAGGAGGGTA TATGCCTCCA
    AGTCCCAGCA CAAGGATGGA CTATATGCAA GGGACGCCTA TAATAGTGCA CCAACAGACT
    TCTTTGTCAC GGCAAAGTAG TTATACAGGG CATGGAACCC TTCCTCGCAT GGGGGTAAAA
    AGGACACCAT CTATTAAGCC AGATGTGCCA CCAAAACCTT CATTTACTTC ACAAAATACA
    TCTGTAAGAC AGCTGAACAA ATATAGCTAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.