Sema6d cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sema6d
Entrez Gene ID	311384
Full Name	semaphorin 6D
General protein information	Preferred Names semaphorin 6D Names semaphorin-6D sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q36

mRNA and Protein(s)

mRNA	Protein	Name
NM_001107768.1	NP_001101238.1	semaphorin-6D precursor
XM_017591743.1	XP_017447232.1	semaphorin-6D isoform X4
XM_008762174.2	XP_008760396.1	semaphorin-6D isoform X5
XM_008762172.2	XP_008760394.1	semaphorin-6D isoform X2
XM_008762173.2	XP_008760395.1	semaphorin-6D isoform X3
XM_008762171.2	XP_008760393.1	semaphorin-6D isoform X1
XM_017591744.1	XP_017447233.1	semaphorin-6D isoform X6
XM_008762175.2	XP_008760397.1	semaphorin-6D isoform X7

Pathways from BioSystems

Homologies

	SEMA6D	XP_001112236.1
Canis lupus familiaris (dog)	SEMA6D	XP_005638436.1
Bos taurus (cattle)	SEMA6D	NP_001178062.1
Homo sapiens (human)	SEMA6D	NP_705871.1
Pan troglodytes (chimpanzee)	SEMA6D	XP_003314766.1
Danio rerio (zebrafish)	sema6dl	NP_001017682.2
Mus musculus (house mouse)	Sema6d	NP_954711.1
Rattus norvegicus (Norway rat)	Sema6d	NP_001101238.1
Gallus gallus (chicken)	SEMA6D	XP_001231859.2
Xenopus tropicalis (tropical clawed frog)	sema6d	NP_001120152.1

Gene Ontology
Bibliography

Related articles in PubMed

Stromal expression of decorin, Semaphorin6D, SPARC, Sprouty1 and Tsukushi in developing prostate and decreased levels of decorin in prostate cancer.
Henke A, Grace OC, Ashley GR, Stewart GD, Riddick AC, Yeun H, O'Donnell M, Anderson RA, Thomson AA
PloS one7(8)e42516(2012)

PlexinA1 signaling directs the segregation of proprioceptive sensory axons in the developing spinal cord.
Yoshida Y, Han B, Mendelsohn M, Jessell TM
Neuron52(5)775-88(2006 Dec)

Dual roles of Sema6D in cardiac morphogenesis through region-specific association of its receptor, Plexin-A1, with off-track and vascular endothelial growth factor receptor type 2.
Toyofuku T, Zhang H, Kumanogoh A, Takegahara N, Suto F, Kamei J, Aoki K, Yabuki M, Hori M, Fujisawa H, Kikutani H
Genes & development18(4)435-47(2004 Feb)

Identification, characterization, and functional study of the two novel human members of the semaphorin gene family.
Qu X, Wei H, Zhai Y, Que H, Chen Q, Tang F, Wu Y, Xing G, Zhu Y, Liu S, Fan M, He F
The Journal of biological chemistry277(38)35574-85(2002 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The spatio-temporal expression of Decorin, Semaphorin6D, SPARC, Spry-1 and Tsukushi in developing prostate is described and observed similar mesenchymal expression patterns in rat and human.
Title: Stromal expression of decorin, Semaphorin6D, SPARC, Sprouty1 and Tsukushi in developing prostate and decreased levels of decorin in prostate cancer.

The following Sema6d gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sema6d cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa05912	NM_001107768.1 Latest version!	Rattus norvegicus semaphorin 6D (Sema6d), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa60402	XM_017591743.1 Latest version!	Rattus norvegicus semaphorin 6D (Sema6d), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa60403	XM_017591744.1 Latest version!	Rattus norvegicus semaphorin 6D (Sema6d), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa46987	XM_008762171.2 Latest version!	Rattus norvegicus semaphorin 6D (Sema6d), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa46988	XM_008762172.2 Latest version!	Rattus norvegicus semaphorin 6D (Sema6d), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa46989	XM_008762173.2 Latest version!	Rattus norvegicus semaphorin 6D (Sema6d), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa46990	XM_008762174.2 Latest version!	Rattus norvegicus semaphorin 6D (Sema6d), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa46991	XM_008762175.2 Latest version!	Rattus norvegicus semaphorin 6D (Sema6d), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sema6d ( NM_001107768.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101238.1 Rattus norvegicus semaphorin 6D (Sema6d), mRNA.

Price & Availability↑

CloneID	ORa05912
Clone ID Related Accession (Same CDS sequence)	NM_001107768.1
Accession Version	NM_001107768.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	semaphorin-6D precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473949.1. On or before Oct 4, 2007 this sequence version replaced XM_230583.4, XM_001077367.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAGGTTCT TTCTGCTGTG GTTCTGTGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCC 
GTCAGCTTCC CTGAGGACGA TGAGCCCCTT AACACGGTTG ACTACCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCATAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG ACATCCCCCA AACAGAGGTG ATCCCGAGCA AGGATGAATG TCACAACTTC 
ATCAAAGTCT TTGTCCCAAG AAATGATGAG ATGGTTTTTG TCTGTGGCAC CAACGCTTTC 
AACCCGATGT GCAGATACTA TAGGTTGAGT ACCTTAGAGT ATGATGGGGA AGAAATTAGT 
GGCCTGGCAC GGTGCCCGTT TGATGCCCGA CAAACCAATG TTGCCCTCTT TGCTGATGGG 
AAACTGTATT CTGCCACAGT GGCTGATTTC CTGGCCAGTG ATGCTGTCAT TTACAGAAGC 
ATGGGTGATG GATCGGCCCT CCGCACAATA AAATACGACT CCAAATGGAT CAAAGAACCA 
CACTTTCTTC ACGCCATAGA ATATGGAAAC TATGTCTATT TCTTCTTCAG AGAAATCGCC 
GTGGAACATA ATAACTTAGG CAAGGCTGTG TACTCCCGAG TGGCCCGCAT TTGTAAAAAC 
GACATGGGTG GCTCACAGCG GGTCCTGGAG AAACACTGGA CTTCCTTCCT GAAGGCTCGG 
CTTAACTGCT CAGTCCCTGG AGATTCCTTT TTCTACTTCG ATGTTCTGCA GTCCATCACA 
GACATCATCC AAATCAATGG CATCCCCACC GTGATCGGGG TCTTCACCAC GCAGCTCAAC 
AGCATTCCTG GCTCTGCAGT CTGTGCCTTT AGCATGGACG ACATTGAGAA AGTGTTCAAA 
GGGCGGTTCA AAGAGCAGAA AACCCCAGAC TCTGTTTGGA CAGCGGTTCC TGAAGACAAA 
GTACCAAAAC CAAGGCCTGG CTGTTGTGCC AAACATGGCC TCGCGGAAGC TTACAAAACC 
TCCATCGACT TTCCAGATGA TACCCTGTCT TTCATCAAGT CCCACCCGCT GATGGACTCC 
GCTGTCCCAC CCATTGCTGA TGAGCCCTGG TTCACAAAGA CTCGGGTCCG GTACAGGCTG 
ACAGCCATCG AAGTGGACCG TTCGGCAGGG CCGTACCAAA ACTACACAGT CATCTTTGTT 
GGCTCTGAGG CCGGCGTGGT GCTTAAAGTT TTGGCAAAGA CCAGTCCTTT CTCTTTGAAC 
GACAGTGTAT TACTCGAAGA GATCGAAGCT TATAACCCAG CCAAGTGCAG CGCCGAGAGT 
GAGGAGGACA GGAAGGTCGT CTCGTTACAG CTGGACAGGG ATCACCATGC TTTATACGTG 
GCCTTCTCCA GCTGCGTGGT CCGCATCCCC CTCAGCCGCT GTGAGCGCTA TGGCTCCTGT 
AAAAAGTCTT GCATTGCATC ACGAGACCCG TACTGTGGTT GGTTAAGCCA GGGAGTGTGT 
GAGAGAGTGA CCTTAGGGAT GCTGCTGTTA ACCGAAGACT TCTTTGCTTT CCATAACCAC 
AGTGCTGGAG GATATGAGCA GGACACCGAG TATGGCAACA CGGCCCACCT AGGGGACTGC 
CACGGTGTAC GGTGGGAAGT CCAGTCTGGA GATTCCAACC AGATGGTCCA CATGAATGTC 
CTCATCACCT GCGTGTTTGC AGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCTGTG 
TACTGCTATC GTGACATGTT TGTTCGGAAG AACAGAAAGA TCCATAAAGA TGCAGAATCG 
GCCCAGTCAT GCACAGATTC CAGTGGAAGC TTTGCCAAGC TGAATGGTCT CTTTGACAGC 
CCCGTCAAGG AGTACCAGCA GAACATCGAT TCACCCAAAC TGTACAGCAA CCTGCTGACC 
AGTCGGAAGG AGCTGCCTCC CAACACGGAT CCAAAGTCCA TGGCCATGGA CCATCGAGGC 
CAGCCTCCAG AGCTGGCTGC TCTCCCCACG CCAGAGTCTA CACCTGTACT CCACCAGAAG 
ACCCTGCAGG CCATGAAGAG CCACTCCGAT AAGGCCCATG GCCATGGTGC TTCAAGGAAG 
GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCGTTAAG TCACGGGCAT 
ATTCCCAGTG CCATCGTTCT TCCAAACGCC ACTCATGACT ACAACACATC CTTCTCAAAC 
TCTAACGCTC ACAAAGCCGA AAAGAAGCTT CAGAACGTTG ATCACCCTCT CACAAAGTCA 
TCCAGTAAGA GGGAACACCG GCGCTCTGTG GACTCCAGAA ACACCCTCAA TGATCTCTTG 
AAGCATCTCA ATGACCCAAA CAGTAACGCC AAAGCCATCA TGGGAGAAAT CCACATGGCC 
CATCAGACCC TCATGCTGGA CCCAGTGGGA CCAATGTCTG AGGTCCCACC CAAGGTTCCT 
AACCGGGAGG CATCCCTATA CTCCCCCCCC TCAACACTCC CCAGAAATAG TCCAACCAAG 
AGAGTAGATG TCCCCACCAC TCCTGGGGTC CCAATGACTT CTCTGGAAAG ACAAAGGGGT 
TATCACAAAA ACTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA 
CCAAACGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGATA TATGCCCACC 
CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTCCA TCTGCAGCCC 
TCCCTCTCCA GACAGAGCAG CTACACCAGT AATGGTACCC TACCCAGGAC GGGACTAAAG 
AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC TCAAACCACA 
TCTGTCAGAC CACTGAACAA ATACACTTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101238.1
CDS	491..3463
Translation	MRFFLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNDIPQTEVIPSKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLSTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVIGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLSFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDRDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSAGGYEQDTEYGNTAHLGDCHGVRWEVQSGDSNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDPKSMAMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSDKAHGHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNVDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNAKAIMGEIHMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	NM_001107768.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus semaphorin 6D (Sema6d), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107768.1

ORF Insert Sequence:

ATGAGGTTCT TTCTGCTGTG GTTCTGTGTG CTGTTCCTTC TGGTCTCCAG GTTACGGGCC 
GTCAGCTTCC CTGAGGACGA TGAGCCCCTT AACACGGTTG ACTACCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG CCCTTCAGGC AACGAATCGC AGCATAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT CTATACAGTG 
AACTTAAATG ACATCCCCCA AACAGAGGTG ATCCCGAGCA AGGATGAATG TCACAACTTC 
ATCAAAGTCT TTGTCCCAAG AAATGATGAG ATGGTTTTTG TCTGTGGCAC CAACGCTTTC 
AACCCGATGT GCAGATACTA TAGGTTGAGT ACCTTAGAGT ATGATGGGGA AGAAATTAGT 
GGCCTGGCAC GGTGCCCGTT TGATGCCCGA CAAACCAATG TTGCCCTCTT TGCTGATGGG 
AAACTGTATT CTGCCACAGT GGCTGATTTC CTGGCCAGTG ATGCTGTCAT TTACAGAAGC 
ATGGGTGATG GATCGGCCCT CCGCACAATA AAATACGACT CCAAATGGAT CAAAGAACCA 
CACTTTCTTC ACGCCATAGA ATATGGAAAC TATGTCTATT TCTTCTTCAG AGAAATCGCC 
GTGGAACATA ATAACTTAGG CAAGGCTGTG TACTCCCGAG TGGCCCGCAT TTGTAAAAAC 
GACATGGGTG GCTCACAGCG GGTCCTGGAG AAACACTGGA CTTCCTTCCT GAAGGCTCGG 
CTTAACTGCT CAGTCCCTGG AGATTCCTTT TTCTACTTCG ATGTTCTGCA GTCCATCACA 
GACATCATCC AAATCAATGG CATCCCCACC GTGATCGGGG TCTTCACCAC GCAGCTCAAC 
AGCATTCCTG GCTCTGCAGT CTGTGCCTTT AGCATGGACG ACATTGAGAA AGTGTTCAAA 
GGGCGGTTCA AAGAGCAGAA AACCCCAGAC TCTGTTTGGA CAGCGGTTCC TGAAGACAAA 
GTACCAAAAC CAAGGCCTGG CTGTTGTGCC AAACATGGCC TCGCGGAAGC TTACAAAACC 
TCCATCGACT TTCCAGATGA TACCCTGTCT TTCATCAAGT CCCACCCGCT GATGGACTCC 
GCTGTCCCAC CCATTGCTGA TGAGCCCTGG TTCACAAAGA CTCGGGTCCG GTACAGGCTG 
ACAGCCATCG AAGTGGACCG TTCGGCAGGG CCGTACCAAA ACTACACAGT CATCTTTGTT 
GGCTCTGAGG CCGGCGTGGT GCTTAAAGTT TTGGCAAAGA CCAGTCCTTT CTCTTTGAAC 
GACAGTGTAT TACTCGAAGA GATCGAAGCT TATAACCCAG CCAAGTGCAG CGCCGAGAGT 
GAGGAGGACA GGAAGGTCGT CTCGTTACAG CTGGACAGGG ATCACCATGC TTTATACGTG 
GCCTTCTCCA GCTGCGTGGT CCGCATCCCC CTCAGCCGCT GTGAGCGCTA TGGCTCCTGT 
AAAAAGTCTT GCATTGCATC ACGAGACCCG TACTGTGGTT GGTTAAGCCA GGGAGTGTGT 
GAGAGAGTGA CCTTAGGGAT GCTGCTGTTA ACCGAAGACT TCTTTGCTTT CCATAACCAC 
AGTGCTGGAG GATATGAGCA GGACACCGAG TATGGCAACA CGGCCCACCT AGGGGACTGC 
CACGGTGTAC GGTGGGAAGT CCAGTCTGGA GATTCCAACC AGATGGTCCA CATGAATGTC 
CTCATCACCT GCGTGTTTGC AGCTTTTGTC TTGGGCGCGT TCATCGCAGG AGTGGCTGTG 
TACTGCTATC GTGACATGTT TGTTCGGAAG AACAGAAAGA TCCATAAAGA TGCAGAATCG 
GCCCAGTCAT GCACAGATTC CAGTGGAAGC TTTGCCAAGC TGAATGGTCT CTTTGACAGC 
CCCGTCAAGG AGTACCAGCA GAACATCGAT TCACCCAAAC TGTACAGCAA CCTGCTGACC 
AGTCGGAAGG AGCTGCCTCC CAACACGGAT CCAAAGTCCA TGGCCATGGA CCATCGAGGC 
CAGCCTCCAG AGCTGGCTGC TCTCCCCACG CCAGAGTCTA CACCTGTACT CCACCAGAAG 
ACCCTGCAGG CCATGAAGAG CCACTCCGAT AAGGCCCATG GCCATGGTGC TTCAAGGAAG 
GAACACCCCC AGTTTTTTCC TTCTAGTCCT CCACCCCATT CCCCGTTAAG TCACGGGCAT 
ATTCCCAGTG CCATCGTTCT TCCAAACGCC ACTCATGACT ACAACACATC CTTCTCAAAC 
TCTAACGCTC ACAAAGCCGA AAAGAAGCTT CAGAACGTTG ATCACCCTCT CACAAAGTCA 
TCCAGTAAGA GGGAACACCG GCGCTCTGTG GACTCCAGAA ACACCCTCAA TGATCTCTTG 
AAGCATCTCA ATGACCCAAA CAGTAACGCC AAAGCCATCA TGGGAGAAAT CCACATGGCC 
CATCAGACCC TCATGCTGGA CCCAGTGGGA CCAATGTCTG AGGTCCCACC CAAGGTTCCT 
AACCGGGAGG CATCCCTATA CTCCCCCCCC TCAACACTCC CCAGAAATAG TCCAACCAAG 
AGAGTAGATG TCCCCACCAC TCCTGGGGTC CCAATGACTT CTCTGGAAAG ACAAAGGGGT 
TATCACAAAA ACTCCTCCCA GAGGCACTCT ATATCTGCCG TGCCTAAAAA CTTAAACTCA 
CCAAACGGTG TTTTGTTATC TAGACAGCCG AGTATGAACC GTGGAGGATA TATGCCCACC 
CCAACAGGGG CGAAGGTGGA CTATATTCAG GGGACACCGG TGAGTGTCCA TCTGCAGCCC 
TCCCTCTCCA GACAGAGCAG CTACACCAGT AATGGTACCC TACCCAGGAC GGGACTAAAG 
AGGACACCAT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CCTTTGTTCC TCAAACCACA 
TCTGTCAGAC CACTGAACAA ATACACTTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_017591743.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447232.1 Rattus norvegicus semaphorin 6D (Sema6d), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa60402
Clone ID Related Accession (Same CDS sequence)	XM_017591743.1
Accession Version	XM_017591743.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	semaphorin-6D isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCAA AGCATCAATA TGCTCACCAG GTGCTTCAGG GAGGCCTGGC TCGGCGCTGC 
CTTTTGTCTG TTCTTGGAGA TAACCTGTCT ACCACTGCCG GGTCTCTTGA TAAATACGGA 
GCTGTAGAGA GAGGTGGGCA AAAATTTGGA CTGGCTACGA GCCAGAACCC AGTTTCACCA 
CAACATAAAT GTCTTTCCAT GGTGCTTTTG TCTTCCAGGA AGCTAAGTGG GACTTCTGAG 
GAGGGAGCTC AGATACCTTC TCGGCCAACC ATGAGGTTCT TTCTGCTGTG GTTCTGTGTG 
CTGTTCCTTC TGGTCTCCAG GTTACGGGCC GTCAGCTTCC CTGAGGACGA TGAGCCCCTT 
AACACGGTTG ACTACCACTA TTCAAGGCAA TATCCGGTTT TTAGAGGACG CCCTTCAGGC 
AACGAATCGC AGCATAGGCT GGACTTTCAG CTGATGTTGA AAATTCGAGA CACACTTTAT 
ATTGCTGGCA GGGATCAAGT CTATACAGTG AACTTAAATG ACATCCCCCA AACAGAGGTG 
ATCCCGAGCA AGAAGCTGAC ATGGAGGTCC AGACAGCAGG ATCGAGAAAA TTGTGCTATG 
AAAGGCAAGC ATAAAGATGA ATGTCACAAC TTCATCAAAG TCTTTGTCCC AAGAAATGAT 
GAGATGGTTT TTGTCTGTGG CACCAACGCT TTCAACCCGA TGTGCAGATA CTATAGGTTG 
AGTACCTTAG AGTATGATGG GGAAGAAATT AGTGGCCTGG CACGGTGCCC GTTTGATGCC 
CGACAAACCA ATGTTGCCCT CTTTGCTGAT GGGAAACTGT ATTCTGCCAC AGTGGCTGAT 
TTCCTGGCCA GTGATGCTGT CATTTACAGA AGCATGGGTG ATGGATCGGC CCTCCGCACA 
ATAAAATACG ACTCCAAATG GATCAAAGAA CCACACTTTC TTCACGCCAT AGAATATGGA 
AACTATGTCT ATTTCTTCTT CAGAGAAATC GCCGTGGAAC ATAATAACTT AGGCAAGGCT 
GTGTACTCCC GAGTGGCCCG CATTTGTAAA AACGACATGG GTGGCTCACA GCGGGTCCTG 
GAGAAACACT GGACTTCCTT CCTGAAGGCT CGGCTTAACT GCTCAGTCCC TGGAGATTCC 
TTTTTCTACT TCGATGTTCT GCAGTCCATC ACAGACATCA TCCAAATCAA TGGCATCCCC 
ACCGTGATCG GGGTCTTCAC CACGCAGCTC AACAGCATTC CTGGCTCTGC AGTCTGTGCC 
TTTAGCATGG ACGACATTGA GAAAGTGTTC AAAGGGCGGT TCAAAGAGCA GAAAACCCCA 
GACTCTGTTT GGACAGCGGT TCCTGAAGAC AAAGTACCAA AACCAAGGCC TGGCTGTTGT 
GCCAAACATG GCCTCGCGGA AGCTTACAAA ACCTCCATCG ACTTTCCAGA TGATACCCTG 
TCTTTCATCA AGTCCCACCC GCTGATGGAC TCCGCTGTCC CACCCATTGC TGATGAGCCC 
TGGTTCACAA AGACTCGGGT CCGGTACAGG CTGACAGCCA TCGAAGTGGA CCGTTCGGCA 
GGGCCGTACC AAAACTACAC AGTCATCTTT GTTGGCTCTG AGGCCGGCGT GGTGCTTAAA 
GTTTTGGCAA AGACCAGTCC TTTCTCTTTG AACGACAGTG TATTACTCGA AGAGATCGAA 
GCTTATAACC CAGCCAAGTG CAGCGCCGAG AGTGAGGAGG ACAGGAAGGT CGTCTCGTTA 
CAGCTGGACA GGGATCACCA TGCTTTATAC GTGGCCTTCT CCAGCTGCGT GGTCCGCATC 
CCCCTCAGCC GCTGTGAGCG CTATGGCTCC TGTAAAAAGT CTTGCATTGC ATCACGAGAC 
CCGTACTGTG GTTGGTTAAG CCAGGGAGTG TGTGAGAGAG TGACCTTAGG GATGCTGCTG 
TTAACCGAAG ACTTCTTTGC TTTCCATAAC CACAGTGCTG GAGGATATGA GCAGGACACC 
GAGTATGGCA ACACGGCCCA CCTAGGGGAC TGCCACGAAA GTTTGCCTAC TTCAACTACA 
CCAGATTACA AAATATTTGG CGGTCCAACA TCTGGTGTAC GGTGGGAAGT CCAGTCTGGA 
GATTCCAACC AGATGGTCCA CATGAATGTC CTCATCACCT GCGTGTTTGC AGCTTTTGTC 
TTGGGCGCGT TCATCGCAGG AGTGGCTGTG TACTGCTATC GTGACATGTT TGTTCGGAAG 
AACAGAAAGA TCCATAAAGA TGCAGAATCG GCCCAGTCAT GCACAGATTC CAGTGGAAGC 
TTTGCCAAGC TGAATGGTCT CTTTGACAGC CCCGTCAAGG AGTACCAGCA GAACATCGAT 
TCACCCAAAC TGTACAGCAA CCTGCTGACC AGTCGGAAGG AGCTGCCTCC CAACACGGAT 
CCAAAGTCCA TGGCCATGGA CCATCGAGGC CAGCCTCCAG AGCTGGCTGC TCTCCCCACG 
CCAGAGTCTA CACCTGTACT CCACCAGAAG ACCCTGCAGG CCATGAAGAG CCACTCCGAT 
AAGGCCCATG GCCATGGTGC TTCAAGGAAG GAACACCCCC AGTTTTTTCC TTCTAGTCCT 
CCACCCCATT CCCCGTTAAG TCACGGGCAT ATTCCCAGTG CCATCGTTCT TCCAAACGCC 
ACTCATGACT ACAACACATC CTTCTCAAAC TCTAACGCTC ACAAAGCCGA AAAGAAGCTT 
CAGAACGTTG ATCACCCTCT CACAAAGTCA TCCAGTAAGA GGGAACACCG GCGCTCTGTG 
GACTCCAGAA ACACCCTCAA TGATCTCTTG AAGCATCTCA ATGACCCAAA CAGTAACGCC 
AAAGCCATCA TGGGAGAAAT CCACATGGCC CATCAGACCC TCATGCTGGA CCCAGTGGGA 
CCAATGTCTG AGGTCCCACC CAAGGTTCCT AACCGGGAGG CATCCCTATA CTCCCCCCCC 
TCAACACTCC CCAGAAATAG TCCAACCAAG AGAGTAGATG TCCCCACCAC TCCTGGGGTC 
CCAATGACTT CTCTGGAAAG ACAAAGGGGT TATCACAAAA ACTCCTCCCA GAGGCACTCT 
ATATCTGCCG TGCCTAAAAA CTTAAACTCA CCAAACGGTG TTTTGTTATC TAGACAGCCG 
AGTATGAACC GTGGAGGATA TATGCCCACC CCAACAGGGG CGAAGGTGGA CTATATTCAG 
GGGACACCGG TGAGTGTCCA TCTGCAGCCC TCCCTCTCCA GACAGAGCAG CTACACCAGT 
AATGGTACCC TACCCAGGAC GGGACTAAAG AGGACACCAT CCTTAAAACC TGATGTGCCA 
CCAAAGCCTT CCTTTGTTCC TCAAACCACA TCTGTCAGAC CACTGAACAA ATACACTTAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447232.1
CDS	3589..6951
Translation	MCPKHQYAHQVLQGGLARRCLLSVLGDNLSTTAGSLDKYGAVERGGQKFGLATSQNPVSPQHKCLSMVLLSSRKLSGTSEEGAQIPSRPTMRFFLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNDIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLSTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVIGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLSFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDRDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSAGGYEQDTEYGNTAHLGDCHESLPTSTTPDYKIFGGPTSGVRWEVQSGDSNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDPKSMAMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSDKAHGHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNVDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNAKAIMGEIHMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_017591743.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus semaphorin 6D (Sema6d), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591743.1

ORF Insert Sequence:

ATGTGCCCAA AGCATCAATA TGCTCACCAG GTGCTTCAGG GAGGCCTGGC TCGGCGCTGC 
CTTTTGTCTG TTCTTGGAGA TAACCTGTCT ACCACTGCCG GGTCTCTTGA TAAATACGGA 
GCTGTAGAGA GAGGTGGGCA AAAATTTGGA CTGGCTACGA GCCAGAACCC AGTTTCACCA 
CAACATAAAT GTCTTTCCAT GGTGCTTTTG TCTTCCAGGA AGCTAAGTGG GACTTCTGAG 
GAGGGAGCTC AGATACCTTC TCGGCCAACC ATGAGGTTCT TTCTGCTGTG GTTCTGTGTG 
CTGTTCCTTC TGGTCTCCAG GTTACGGGCC GTCAGCTTCC CTGAGGACGA TGAGCCCCTT 
AACACGGTTG ACTACCACTA TTCAAGGCAA TATCCGGTTT TTAGAGGACG CCCTTCAGGC 
AACGAATCGC AGCATAGGCT GGACTTTCAG CTGATGTTGA AAATTCGAGA CACACTTTAT 
ATTGCTGGCA GGGATCAAGT CTATACAGTG AACTTAAATG ACATCCCCCA AACAGAGGTG 
ATCCCGAGCA AGAAGCTGAC ATGGAGGTCC AGACAGCAGG ATCGAGAAAA TTGTGCTATG 
AAAGGCAAGC ATAAAGATGA ATGTCACAAC TTCATCAAAG TCTTTGTCCC AAGAAATGAT 
GAGATGGTTT TTGTCTGTGG CACCAACGCT TTCAACCCGA TGTGCAGATA CTATAGGTTG 
AGTACCTTAG AGTATGATGG GGAAGAAATT AGTGGCCTGG CACGGTGCCC GTTTGATGCC 
CGACAAACCA ATGTTGCCCT CTTTGCTGAT GGGAAACTGT ATTCTGCCAC AGTGGCTGAT 
TTCCTGGCCA GTGATGCTGT CATTTACAGA AGCATGGGTG ATGGATCGGC CCTCCGCACA 
ATAAAATACG ACTCCAAATG GATCAAAGAA CCACACTTTC TTCACGCCAT AGAATATGGA 
AACTATGTCT ATTTCTTCTT CAGAGAAATC GCCGTGGAAC ATAATAACTT AGGCAAGGCT 
GTGTACTCCC GAGTGGCCCG CATTTGTAAA AACGACATGG GTGGCTCACA GCGGGTCCTG 
GAGAAACACT GGACTTCCTT CCTGAAGGCT CGGCTTAACT GCTCAGTCCC TGGAGATTCC 
TTTTTCTACT TCGATGTTCT GCAGTCCATC ACAGACATCA TCCAAATCAA TGGCATCCCC 
ACCGTGATCG GGGTCTTCAC CACGCAGCTC AACAGCATTC CTGGCTCTGC AGTCTGTGCC 
TTTAGCATGG ACGACATTGA GAAAGTGTTC AAAGGGCGGT TCAAAGAGCA GAAAACCCCA 
GACTCTGTTT GGACAGCGGT TCCTGAAGAC AAAGTACCAA AACCAAGGCC TGGCTGTTGT 
GCCAAACATG GCCTCGCGGA AGCTTACAAA ACCTCCATCG ACTTTCCAGA TGATACCCTG 
TCTTTCATCA AGTCCCACCC GCTGATGGAC TCCGCTGTCC CACCCATTGC TGATGAGCCC 
TGGTTCACAA AGACTCGGGT CCGGTACAGG CTGACAGCCA TCGAAGTGGA CCGTTCGGCA 
GGGCCGTACC AAAACTACAC AGTCATCTTT GTTGGCTCTG AGGCCGGCGT GGTGCTTAAA 
GTTTTGGCAA AGACCAGTCC TTTCTCTTTG AACGACAGTG TATTACTCGA AGAGATCGAA 
GCTTATAACC CAGCCAAGTG CAGCGCCGAG AGTGAGGAGG ACAGGAAGGT CGTCTCGTTA 
CAGCTGGACA GGGATCACCA TGCTTTATAC GTGGCCTTCT CCAGCTGCGT GGTCCGCATC 
CCCCTCAGCC GCTGTGAGCG CTATGGCTCC TGTAAAAAGT CTTGCATTGC ATCACGAGAC 
CCGTACTGTG GTTGGTTAAG CCAGGGAGTG TGTGAGAGAG TGACCTTAGG GATGCTGCTG 
TTAACCGAAG ACTTCTTTGC TTTCCATAAC CACAGTGCTG GAGGATATGA GCAGGACACC 
GAGTATGGCA ACACGGCCCA CCTAGGGGAC TGCCACGAAA GTTTGCCTAC TTCAACTACA 
CCAGATTACA AAATATTTGG CGGTCCAACA TCTGGTGTAC GGTGGGAAGT CCAGTCTGGA 
GATTCCAACC AGATGGTCCA CATGAATGTC CTCATCACCT GCGTGTTTGC AGCTTTTGTC 
TTGGGCGCGT TCATCGCAGG AGTGGCTGTG TACTGCTATC GTGACATGTT TGTTCGGAAG 
AACAGAAAGA TCCATAAAGA TGCAGAATCG GCCCAGTCAT GCACAGATTC CAGTGGAAGC 
TTTGCCAAGC TGAATGGTCT CTTTGACAGC CCCGTCAAGG AGTACCAGCA GAACATCGAT 
TCACCCAAAC TGTACAGCAA CCTGCTGACC AGTCGGAAGG AGCTGCCTCC CAACACGGAT 
CCAAAGTCCA TGGCCATGGA CCATCGAGGC CAGCCTCCAG AGCTGGCTGC TCTCCCCACG 
CCAGAGTCTA CACCTGTACT CCACCAGAAG ACCCTGCAGG CCATGAAGAG CCACTCCGAT 
AAGGCCCATG GCCATGGTGC TTCAAGGAAG GAACACCCCC AGTTTTTTCC TTCTAGTCCT 
CCACCCCATT CCCCGTTAAG TCACGGGCAT ATTCCCAGTG CCATCGTTCT TCCAAACGCC 
ACTCATGACT ACAACACATC CTTCTCAAAC TCTAACGCTC ACAAAGCCGA AAAGAAGCTT 
CAGAACGTTG ATCACCCTCT CACAAAGTCA TCCAGTAAGA GGGAACACCG GCGCTCTGTG 
GACTCCAGAA ACACCCTCAA TGATCTCTTG AAGCATCTCA ATGACCCAAA CAGTAACGCC 
AAAGCCATCA TGGGAGAAAT CCACATGGCC CATCAGACCC TCATGCTGGA CCCAGTGGGA 
CCAATGTCTG AGGTCCCACC CAAGGTTCCT AACCGGGAGG CATCCCTATA CTCCCCCCCC 
TCAACACTCC CCAGAAATAG TCCAACCAAG AGAGTAGATG TCCCCACCAC TCCTGGGGTC 
CCAATGACTT CTCTGGAAAG ACAAAGGGGT TATCACAAAA ACTCCTCCCA GAGGCACTCT 
ATATCTGCCG TGCCTAAAAA CTTAAACTCA CCAAACGGTG TTTTGTTATC TAGACAGCCG 
AGTATGAACC GTGGAGGATA TATGCCCACC CCAACAGGGG CGAAGGTGGA CTATATTCAG 
GGGACACCGG TGAGTGTCCA TCTGCAGCCC TCCCTCTCCA GACAGAGCAG CTACACCAGT 
AATGGTACCC TACCCAGGAC GGGACTAAAG AGGACACCAT CCTTAAAACC TGATGTGCCA 
CCAAAGCCTT CCTTTGTTCC TCAAACCACA TCTGTCAGAC CACTGAACAA ATACACTTAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_017591744.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447233.1 Rattus norvegicus semaphorin 6D (Sema6d), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa60403
Clone ID Related Accession (Same CDS sequence)	XM_017591744.1
Accession Version	XM_017591744.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	semaphorin-6D isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCAA AGCATCAATA TGCTCACCAG GTGCTTCAGG GAGGCCTGGC TCGGCGCTGC 
CTTTTGTCTG TTCTTGGAGA TAACCTGTCT ACCACTGCCG GGTCTCTTGA TAAATACGGA 
GCTGTAGAGA GAGGTGGGCA AAAATTTGGA CTGGCTACGA GCCAGAACCC AGTTTCACCA 
CAACATAAAT GTCTTTCCAT GGTGCTTTTG TCTTCCAGGA AGCTAAGTGG GACTTCTGAG 
GAGGGAGCTC AGATACCTTC TCGGCCAACC ATGAGGTTCT TTCTGCTGTG GTTCTGTGTG 
CTGTTCCTTC TGGTCTCCAG GTTACGGGCC GTCAGCTTCC CTGAGGACGA TGAGCCCCTT 
AACACGGTTG ACTACCACTA TTCAAGGCAA TATCCGGTTT TTAGAGGACG CCCTTCAGGC 
AACGAATCGC AGCATAGGCT GGACTTTCAG CTGATGTTGA AAATTCGAGA CACACTTTAT 
ATTGCTGGCA GGGATCAAGT CTATACAGTG AACTTAAATG ACATCCCCCA AACAGAGGTG 
ATCCCGAGCA AGAAGCTGAC ATGGAGGTCC AGACAGCAGG ATCGAGAAAA TTGTGCTATG 
AAAGGCAAGC ATAAAGATGA ATGTCACAAC TTCATCAAAG TCTTTGTCCC AAGAAATGAT 
GAGATGGTTT TTGTCTGTGG CACCAACGCT TTCAACCCGA TGTGCAGATA CTATAGGTTG 
AGTACCTTAG AGTATGATGG GGAAGAAATT AGTGGCCTGG CACGGTGCCC GTTTGATGCC 
CGACAAACCA ATGTTGCCCT CTTTGCTGAT GGGAAACTGT ATTCTGCCAC AGTGGCTGAT 
TTCCTGGCCA GTGATGCTGT CATTTACAGA AGCATGGGTG ATGGATCGGC CCTCCGCACA 
ATAAAATACG ACTCCAAATG GATCAAAGAA CCACACTTTC TTCACGCCAT AGAATATGGA 
AACTATGTCT ATTTCTTCTT CAGAGAAATC GCCGTGGAAC ATAATAACTT AGGCAAGGCT 
GTGTACTCCC GAGTGGCCCG CATTTGTAAA AACGACATGG GTGGCTCACA GCGGGTCCTG 
GAGAAACACT GGACTTCCTT CCTGAAGGCT CGGCTTAACT GCTCAGTCCC TGGAGATTCC 
TTTTTCTACT TCGATGTTCT GCAGTCCATC ACAGACATCA TCCAAATCAA TGGCATCCCC 
ACCGTGATCG GGGTCTTCAC CACGCAGCTC AACAGCATTC CTGGCTCTGC AGTCTGTGCC 
TTTAGCATGG ACGACATTGA GAAAGTGTTC AAAGGGCGGT TCAAAGAGCA GAAAACCCCA 
GACTCTGTTT GGACAGCGGT TCCTGAAGAC AAAGTACCAA AACCAAGGCC TGGCTGTTGT 
GCCAAACATG GCCTCGCGGA AGCTTACAAA ACCTCCATCG ACTTTCCAGA TGATACCCTG 
TCTTTCATCA AGTCCCACCC GCTGATGGAC TCCGCTGTCC CACCCATTGC TGATGAGCCC 
TGGTTCACAA AGACTCGGGT CCGGTACAGG CTGACAGCCA TCGAAGTGGA CCGTTCGGCA 
GGGCCGTACC AAAACTACAC AGTCATCTTT GTTGGCTCTG AGGCCGGCGT GGTGCTTAAA 
GTTTTGGCAA AGACCAGTCC TTTCTCTTTG AACGACAGTG TATTACTCGA AGAGATCGAA 
GCTTATAACC CAGCCAAGTG CAGCGCCGAG AGTGAGGAGG ACAGGAAGGT CGTCTCGTTA 
CAGCTGGACA GGGATCACCA TGCTTTATAC GTGGCCTTCT CCAGCTGCGT GGTCCGCATC 
CCCCTCAGCC GCTGTGAGCG CTATGGCTCC TGTAAAAAGT CTTGCATTGC ATCACGAGAC 
CCGTACTGTG GTTGGTTAAG CCAGGGAGTG TGTGAGAGAG TGACCTTAGG GATGCTGCTG 
TTAACCGAAG ACTTCTTTGC TTTCCATAAC CACAGTGCTG GAGGATATGA GCAGGACACC 
GAGTATGGCA ACACGGCCCA CCTAGGGGAC TGCCACGGTG TACGGTGGGA AGTCCAGTCT 
GGAGATTCCA ACCAGATGGT CCACATGAAT GTCCTCATCA CCTGCGTGTT TGCAGCTTTT 
GTCTTGGGCG CGTTCATCGC AGGAGTGGCT GTGTACTGCT ATCGTGACAT GTTTGTTCGG 
AAGAACAGAA AGATCCATAA AGATGCAGAA TCGGCCCAGT CATGCACAGA TTCCAGTGGA 
AGCTTTGCCA AGCTGAATGG TCTCTTTGAC AGCCCCGTCA AGGAGTACCA GCAGAACATC 
GATTCACCCA AACTGTACAG CAACCTGCTG ACCAGTCGGA AGGAGCTGCC TCCCAACACG 
GATCCAAAGT CCATGGCCAT GGACCATCGA GGCCAGCCTC CAGAGCTGGC TGCTCTCCCC 
ACGCCAGAGT CTACACCTGT ACTCCACCAG AAGACCCTGC AGGCCATGAA GAGCCACTCC 
GATAAGGCCC ATGGCCATGG TGCTTCAAGG AAGGAACACC CCCAGTTTTT TCCTTCTAGT 
CCTCCACCCC ATTCCCCGTT AAGTCACGGG CATATTCCCA GTGCCATCGT TCTTCCAAAC 
GCCACTCATG ACTACAACAC ATCCTTCTCA AACTCTAACG CTCACAAAGC CGAAAAGAAG 
CTTCAGAACG TTGATCACCC TCTCACAAAG TCATCCAGTA AGAGGGAACA CCGGCGCTCT 
GTGGACTCCA GAAACACCCT CAATGATCTC TTGAAGCATC TCAATGACCC AAACAGTAAC 
GCCAAAGCCA TCATGGGAGA AATCCACATG GCCCATCAGA CCCTCATGCT GGACCCAGTG 
GGACCAATGT CTGAGGTCCC ACCCAAGGTT CCTAACCGGG AGGCATCCCT ATACTCCCCC 
CCCTCAACAC TCCCCAGAAA TAGTCCAACC AAGAGAGTAG ATGTCCCCAC CACTCCTGGG 
GTCCCAATGA CTTCTCTGGA AAGACAAAGG GGTTATCACA AAAACTCCTC CCAGAGGCAC 
TCTATATCTG CCGTGCCTAA AAACTTAAAC TCACCAAACG GTGTTTTGTT ATCTAGACAG 
CCGAGTATGA ACCGTGGAGG ATATATGCCC ACCCCAACAG GGGCGAAGGT GGACTATATT 
CAGGGGACAC CGGTGAGTGT CCATCTGCAG CCCTCCCTCT CCAGACAGAG CAGCTACACC 
AGTAATGGTA CCCTACCCAG GACGGGACTA AAGAGGACAC CATCCTTAAA ACCTGATGTG 
CCACCAAAGC CTTCCTTTGT TCCTCAAACC ACATCTGTCA GACCACTGAA CAAATACACT 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447233.1
CDS	3589..6894
Translation	MCPKHQYAHQVLQGGLARRCLLSVLGDNLSTTAGSLDKYGAVERGGQKFGLATSQNPVSPQHKCLSMVLLSSRKLSGTSEEGAQIPSRPTMRFFLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNDIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLSTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVIGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLSFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDRDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLLLTEDFFAFHNHSAGGYEQDTEYGNTAHLGDCHGVRWEVQSGDSNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDPKSMAMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSDKAHGHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNVDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNAKAIMGEIHMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_017591744.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus semaphorin 6D (Sema6d), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591744.1

ORF Insert Sequence:

ATGTGCCCAA AGCATCAATA TGCTCACCAG GTGCTTCAGG GAGGCCTGGC TCGGCGCTGC 
CTTTTGTCTG TTCTTGGAGA TAACCTGTCT ACCACTGCCG GGTCTCTTGA TAAATACGGA 
GCTGTAGAGA GAGGTGGGCA AAAATTTGGA CTGGCTACGA GCCAGAACCC AGTTTCACCA 
CAACATAAAT GTCTTTCCAT GGTGCTTTTG TCTTCCAGGA AGCTAAGTGG GACTTCTGAG 
GAGGGAGCTC AGATACCTTC TCGGCCAACC ATGAGGTTCT TTCTGCTGTG GTTCTGTGTG 
CTGTTCCTTC TGGTCTCCAG GTTACGGGCC GTCAGCTTCC CTGAGGACGA TGAGCCCCTT 
AACACGGTTG ACTACCACTA TTCAAGGCAA TATCCGGTTT TTAGAGGACG CCCTTCAGGC 
AACGAATCGC AGCATAGGCT GGACTTTCAG CTGATGTTGA AAATTCGAGA CACACTTTAT 
ATTGCTGGCA GGGATCAAGT CTATACAGTG AACTTAAATG ACATCCCCCA AACAGAGGTG 
ATCCCGAGCA AGAAGCTGAC ATGGAGGTCC AGACAGCAGG ATCGAGAAAA TTGTGCTATG 
AAAGGCAAGC ATAAAGATGA ATGTCACAAC TTCATCAAAG TCTTTGTCCC AAGAAATGAT 
GAGATGGTTT TTGTCTGTGG CACCAACGCT TTCAACCCGA TGTGCAGATA CTATAGGTTG 
AGTACCTTAG AGTATGATGG GGAAGAAATT AGTGGCCTGG CACGGTGCCC GTTTGATGCC 
CGACAAACCA ATGTTGCCCT CTTTGCTGAT GGGAAACTGT ATTCTGCCAC AGTGGCTGAT 
TTCCTGGCCA GTGATGCTGT CATTTACAGA AGCATGGGTG ATGGATCGGC CCTCCGCACA 
ATAAAATACG ACTCCAAATG GATCAAAGAA CCACACTTTC TTCACGCCAT AGAATATGGA 
AACTATGTCT ATTTCTTCTT CAGAGAAATC GCCGTGGAAC ATAATAACTT AGGCAAGGCT 
GTGTACTCCC GAGTGGCCCG CATTTGTAAA AACGACATGG GTGGCTCACA GCGGGTCCTG 
GAGAAACACT GGACTTCCTT CCTGAAGGCT CGGCTTAACT GCTCAGTCCC TGGAGATTCC 
TTTTTCTACT TCGATGTTCT GCAGTCCATC ACAGACATCA TCCAAATCAA TGGCATCCCC 
ACCGTGATCG GGGTCTTCAC CACGCAGCTC AACAGCATTC CTGGCTCTGC AGTCTGTGCC 
TTTAGCATGG ACGACATTGA GAAAGTGTTC AAAGGGCGGT TCAAAGAGCA GAAAACCCCA 
GACTCTGTTT GGACAGCGGT TCCTGAAGAC AAAGTACCAA AACCAAGGCC TGGCTGTTGT 
GCCAAACATG GCCTCGCGGA AGCTTACAAA ACCTCCATCG ACTTTCCAGA TGATACCCTG 
TCTTTCATCA AGTCCCACCC GCTGATGGAC TCCGCTGTCC CACCCATTGC TGATGAGCCC 
TGGTTCACAA AGACTCGGGT CCGGTACAGG CTGACAGCCA TCGAAGTGGA CCGTTCGGCA 
GGGCCGTACC AAAACTACAC AGTCATCTTT GTTGGCTCTG AGGCCGGCGT GGTGCTTAAA 
GTTTTGGCAA AGACCAGTCC TTTCTCTTTG AACGACAGTG TATTACTCGA AGAGATCGAA 
GCTTATAACC CAGCCAAGTG CAGCGCCGAG AGTGAGGAGG ACAGGAAGGT CGTCTCGTTA 
CAGCTGGACA GGGATCACCA TGCTTTATAC GTGGCCTTCT CCAGCTGCGT GGTCCGCATC 
CCCCTCAGCC GCTGTGAGCG CTATGGCTCC TGTAAAAAGT CTTGCATTGC ATCACGAGAC 
CCGTACTGTG GTTGGTTAAG CCAGGGAGTG TGTGAGAGAG TGACCTTAGG GATGCTGCTG 
TTAACCGAAG ACTTCTTTGC TTTCCATAAC CACAGTGCTG GAGGATATGA GCAGGACACC 
GAGTATGGCA ACACGGCCCA CCTAGGGGAC TGCCACGGTG TACGGTGGGA AGTCCAGTCT 
GGAGATTCCA ACCAGATGGT CCACATGAAT GTCCTCATCA CCTGCGTGTT TGCAGCTTTT 
GTCTTGGGCG CGTTCATCGC AGGAGTGGCT GTGTACTGCT ATCGTGACAT GTTTGTTCGG 
AAGAACAGAA AGATCCATAA AGATGCAGAA TCGGCCCAGT CATGCACAGA TTCCAGTGGA 
AGCTTTGCCA AGCTGAATGG TCTCTTTGAC AGCCCCGTCA AGGAGTACCA GCAGAACATC 
GATTCACCCA AACTGTACAG CAACCTGCTG ACCAGTCGGA AGGAGCTGCC TCCCAACACG 
GATCCAAAGT CCATGGCCAT GGACCATCGA GGCCAGCCTC CAGAGCTGGC TGCTCTCCCC 
ACGCCAGAGT CTACACCTGT ACTCCACCAG AAGACCCTGC AGGCCATGAA GAGCCACTCC 
GATAAGGCCC ATGGCCATGG TGCTTCAAGG AAGGAACACC CCCAGTTTTT TCCTTCTAGT 
CCTCCACCCC ATTCCCCGTT AAGTCACGGG CATATTCCCA GTGCCATCGT TCTTCCAAAC 
GCCACTCATG ACTACAACAC ATCCTTCTCA AACTCTAACG CTCACAAAGC CGAAAAGAAG 
CTTCAGAACG TTGATCACCC TCTCACAAAG TCATCCAGTA AGAGGGAACA CCGGCGCTCT 
GTGGACTCCA GAAACACCCT CAATGATCTC TTGAAGCATC TCAATGACCC AAACAGTAAC 
GCCAAAGCCA TCATGGGAGA AATCCACATG GCCCATCAGA CCCTCATGCT GGACCCAGTG 
GGACCAATGT CTGAGGTCCC ACCCAAGGTT CCTAACCGGG AGGCATCCCT ATACTCCCCC 
CCCTCAACAC TCCCCAGAAA TAGTCCAACC AAGAGAGTAG ATGTCCCCAC CACTCCTGGG 
GTCCCAATGA CTTCTCTGGA AAGACAAAGG GGTTATCACA AAAACTCCTC CCAGAGGCAC 
TCTATATCTG CCGTGCCTAA AAACTTAAAC TCACCAAACG GTGTTTTGTT ATCTAGACAG 
CCGAGTATGA ACCGTGGAGG ATATATGCCC ACCCCAACAG GGGCGAAGGT GGACTATATT 
CAGGGGACAC CGGTGAGTGT CCATCTGCAG CCCTCCCTCT CCAGACAGAG CAGCTACACC 
AGTAATGGTA CCCTACCCAG GACGGGACTA AAGAGGACAC CATCCTTAAA ACCTGATGTG 
CCACCAAAGC CTTCCTTTGT TCCTCAAACC ACATCTGTCA GACCACTGAA CAAATACACT 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_008762171.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760393.1 Rattus norvegicus semaphorin 6D (Sema6d), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa46987
Clone ID Related Accession (Same CDS sequence)	XM_008762171.2
Accession Version	XM_008762171.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	semaphorin-6D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762171.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCAA AGCATCAATA TGCTCACCAG GTGCTTCAGG GAGGCCTGGC TCGGCGCTGC 
CTTTTGTCTG TTCTTGGAGA TAACCTGTCT ACCACTGCCG GGTCTCTTGA TAAATACGGA 
GCTGTAGAGA GAGGTGGGCA AAAATTTGGA CTGGCTACGA GCCAGAACCC AGTTTCACCA 
CAACATAAAT GTCTTTCCAT GGTGCTTTTG TCTTCCAGGA AGCTAAGTGG GACTTCTGAG 
GAGGGAGCTC AGATACCTTC TCGGCCAACC ATGAGGTTCT TTCTGCTGTG GTTCTGTGTG 
CTGTTCCTTC TGGTCTCCAG GTTACGGGCC GTCAGCTTCC CTGAGGACGA TGAGCCCCTT 
AACACGGTTG ACTACCACTA TTCAAGGCAA TATCCGGTTT TTAGAGGACG CCCTTCAGGC 
AACGAATCGC AGCATAGGCT GGACTTTCAG CTGATGTTGA AAATTCGAGA CACACTTTAT 
ATTGCTGGCA GGGATCAAGT CTATACAGTG AACTTAAATG ACATCCCCCA AACAGAGGTG 
ATCCCGAGCA AGAAGCTGAC ATGGAGGTCC AGACAGCAGG ATCGAGAAAA TTGTGCTATG 
AAAGGCAAGC ATAAAGATGA ATGTCACAAC TTCATCAAAG TCTTTGTCCC AAGAAATGAT 
GAGATGGTTT TTGTCTGTGG CACCAACGCT TTCAACCCGA TGTGCAGATA CTATAGGTTG 
AGTACCTTAG AGTATGATGG GGAAGAAATT AGTGGCCTGG CACGGTGCCC GTTTGATGCC 
CGACAAACCA ATGTTGCCCT CTTTGCTGAT GGGAAACTGT ATTCTGCCAC AGTGGCTGAT 
TTCCTGGCCA GTGATGCTGT CATTTACAGA AGCATGGGTG ATGGATCGGC CCTCCGCACA 
ATAAAATACG ACTCCAAATG GATCAAAGAA CCACACTTTC TTCACGCCAT AGAATATGGA 
AACTATGTCT ATTTCTTCTT CAGAGAAATC GCCGTGGAAC ATAATAACTT AGGCAAGGCT 
GTGTACTCCC GAGTGGCCCG CATTTGTAAA AACGACATGG GTGGCTCACA GCGGGTCCTG 
GAGAAACACT GGACTTCCTT CCTGAAGGCT CGGCTTAACT GCTCAGTCCC TGGAGATTCC 
TTTTTCTACT TCGATGTTCT GCAGTCCATC ACAGACATCA TCCAAATCAA TGGCATCCCC 
ACCGTGATCG GGGTCTTCAC CACGCAGCTC AACAGCATTC CTGGCTCTGC AGTCTGTGCC 
TTTAGCATGG ACGACATTGA GAAAGTGTTC AAAGGGCGGT TCAAAGAGCA GAAAACCCCA 
GACTCTGTTT GGACAGCGGT TCCTGAAGAC AAAGTACCAA AACCAAGGCC TGGCTGTTGT 
GCCAAACATG GCCTCGCGGA AGCTTACAAA ACCTCCATCG ACTTTCCAGA TGATACCCTG 
TCTTTCATCA AGTCCCACCC GCTGATGGAC TCCGCTGTCC CACCCATTGC TGATGAGCCC 
TGGTTCACAA AGACTCGGGT CCGGTACAGG CTGACAGCCA TCGAAGTGGA CCGTTCGGCA 
GGGCCGTACC AAAACTACAC AGTCATCTTT GTTGGCTCTG AGGCCGGCGT GGTGCTTAAA 
GTTTTGGCAA AGACCAGTCC TTTCTCTTTG AACGACAGTG TATTACTCGA AGAGATCGAA 
GCTTATAACC CAGCCAAGTG CAGCGCCGAG AGTGAGGAGG ACAGGAAGGT CGTCTCGTTA 
CAGCTGGACA GGGATCACCA TGCTTTATAC GTGGCCTTCT CCAGCTGCGT GGTCCGCATC 
CCCCTCAGCC GCTGTGAGCG CTATGGCTCC TGTAAAAAGT CTTGCATTGC ATCACGAGAC 
CCGTACTGTG GTTGGTTAAG CCAGGGAGTG TGTGAGAGAG TGACCTTAGG GATGCTTGCT 
GGAGGATATG AGCAGGACAC CGAGTATGGC AACACGGCCC ACCTAGGGGA CTGCCACGAA 
AGTTTGCCTA CTTCAACTAC ACCAGATTAC AAAATATTTG GCGGTCCAAC ATCTGACATG 
GAGGTACCCT CATCTTCTGT TACCACTGTG GCAAGTAGCC CAGAAATTAC ATCTAAAGTG 
ATTGATACCT GGAGACCTAA ACTGACGAGC TCCCGGAAAT TTGTAGTTCA AGATGACCCA 
AACACTTCCG ATTTTACTGA TACTATATCA GGTATCCCAA AGGGTGTACG GTGGGAAGTC 
CAGTCTGGAG ATTCCAACCA GATGGTCCAC ATGAATGTCC TCATCACCTG CGTGTTTGCA 
GCTTTTGTCT TGGGCGCGTT CATCGCAGGA GTGGCTGTGT ACTGCTATCG TGACATGTTT 
GTTCGGAAGA ACAGAAAGAT CCATAAAGAT GCAGAATCGG CCCAGTCATG CACAGATTCC 
AGTGGAAGCT TTGCCAAGCT GAATGGTCTC TTTGACAGCC CCGTCAAGGA GTACCAGCAG 
AACATCGATT CACCCAAACT GTACAGCAAC CTGCTGACCA GTCGGAAGGA GCTGCCTCCC 
AACACGGATC CAAAGTCCAT GGCCATGGAC CATCGAGGCC AGCCTCCAGA GCTGGCTGCT 
CTCCCCACGC CAGAGTCTAC ACCTGTACTC CACCAGAAGA CCCTGCAGGC CATGAAGAGC 
CACTCCGATA AGGCCCATGG CCATGGTGCT TCAAGGAAGG AACACCCCCA GTTTTTTCCT 
TCTAGTCCTC CACCCCATTC CCCGTTAAGT CACGGGCATA TTCCCAGTGC CATCGTTCTT 
CCAAACGCCA CTCATGACTA CAACACATCC TTCTCAAACT CTAACGCTCA CAAAGCCGAA 
AAGAAGCTTC AGAACGTTGA TCACCCTCTC ACAAAGTCAT CCAGTAAGAG GGAACACCGG 
CGCTCTGTGG ACTCCAGAAA CACCCTCAAT GATCTCTTGA AGCATCTCAA TGACCCAAAC 
AGTAACGCCA AAGCCATCAT GGGAGAAATC CACATGGCCC ATCAGACCCT CATGCTGGAC 
CCAGTGGGAC CAATGTCTGA GGTCCCACCC AAGGTTCCTA ACCGGGAGGC ATCCCTATAC 
TCCCCCCCCT CAACACTCCC CAGAAATAGT CCAACCAAGA GAGTAGATGT CCCCACCACT 
CCTGGGGTCC CAATGACTTC TCTGGAAAGA CAAAGGGGTT ATCACAAAAA CTCCTCCCAG 
AGGCACTCTA TATCTGCCGT GCCTAAAAAC TTAAACTCAC CAAACGGTGT TTTGTTATCT 
AGACAGCCGA GTATGAACCG TGGAGGATAT ATGCCCACCC CAACAGGGGC GAAGGTGGAC 
TATATTCAGG GGACACCGGT GAGTGTCCAT CTGCAGCCCT CCCTCTCCAG ACAGAGCAGC 
TACACCAGTA ATGGTACCCT ACCCAGGACG GGACTAAAGA GGACACCATC CTTAAAACCT 
GATGTGCCAC CAAAGCCTTC CTTTGTTCCT CAAACCACAT CTGTCAGACC ACTGAACAAA 
TACACTTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760393.1
CDS	3589..7080
Translation	MCPKHQYAHQVLQGGLARRCLLSVLGDNLSTTAGSLDKYGAVERGGQKFGLATSQNPVSPQHKCLSMVLLSSRKLSGTSEEGAQIPSRPTMRFFLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNDIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLSTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVIGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLSFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDRDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLAGGYEQDTEYGNTAHLGDCHESLPTSTTPDYKIFGGPTSDMEVPSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGDSNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDPKSMAMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSDKAHGHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNVDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNAKAIMGEIHMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_008762171.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus semaphorin 6D (Sema6d), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762171.2

ORF Insert Sequence:

ATGTGCCCAA AGCATCAATA TGCTCACCAG GTGCTTCAGG GAGGCCTGGC TCGGCGCTGC 
CTTTTGTCTG TTCTTGGAGA TAACCTGTCT ACCACTGCCG GGTCTCTTGA TAAATACGGA 
GCTGTAGAGA GAGGTGGGCA AAAATTTGGA CTGGCTACGA GCCAGAACCC AGTTTCACCA 
CAACATAAAT GTCTTTCCAT GGTGCTTTTG TCTTCCAGGA AGCTAAGTGG GACTTCTGAG 
GAGGGAGCTC AGATACCTTC TCGGCCAACC ATGAGGTTCT TTCTGCTGTG GTTCTGTGTG 
CTGTTCCTTC TGGTCTCCAG GTTACGGGCC GTCAGCTTCC CTGAGGACGA TGAGCCCCTT 
AACACGGTTG ACTACCACTA TTCAAGGCAA TATCCGGTTT TTAGAGGACG CCCTTCAGGC 
AACGAATCGC AGCATAGGCT GGACTTTCAG CTGATGTTGA AAATTCGAGA CACACTTTAT 
ATTGCTGGCA GGGATCAAGT CTATACAGTG AACTTAAATG ACATCCCCCA AACAGAGGTG 
ATCCCGAGCA AGAAGCTGAC ATGGAGGTCC AGACAGCAGG ATCGAGAAAA TTGTGCTATG 
AAAGGCAAGC ATAAAGATGA ATGTCACAAC TTCATCAAAG TCTTTGTCCC AAGAAATGAT 
GAGATGGTTT TTGTCTGTGG CACCAACGCT TTCAACCCGA TGTGCAGATA CTATAGGTTG 
AGTACCTTAG AGTATGATGG GGAAGAAATT AGTGGCCTGG CACGGTGCCC GTTTGATGCC 
CGACAAACCA ATGTTGCCCT CTTTGCTGAT GGGAAACTGT ATTCTGCCAC AGTGGCTGAT 
TTCCTGGCCA GTGATGCTGT CATTTACAGA AGCATGGGTG ATGGATCGGC CCTCCGCACA 
ATAAAATACG ACTCCAAATG GATCAAAGAA CCACACTTTC TTCACGCCAT AGAATATGGA 
AACTATGTCT ATTTCTTCTT CAGAGAAATC GCCGTGGAAC ATAATAACTT AGGCAAGGCT 
GTGTACTCCC GAGTGGCCCG CATTTGTAAA AACGACATGG GTGGCTCACA GCGGGTCCTG 
GAGAAACACT GGACTTCCTT CCTGAAGGCT CGGCTTAACT GCTCAGTCCC TGGAGATTCC 
TTTTTCTACT TCGATGTTCT GCAGTCCATC ACAGACATCA TCCAAATCAA TGGCATCCCC 
ACCGTGATCG GGGTCTTCAC CACGCAGCTC AACAGCATTC CTGGCTCTGC AGTCTGTGCC 
TTTAGCATGG ACGACATTGA GAAAGTGTTC AAAGGGCGGT TCAAAGAGCA GAAAACCCCA 
GACTCTGTTT GGACAGCGGT TCCTGAAGAC AAAGTACCAA AACCAAGGCC TGGCTGTTGT 
GCCAAACATG GCCTCGCGGA AGCTTACAAA ACCTCCATCG ACTTTCCAGA TGATACCCTG 
TCTTTCATCA AGTCCCACCC GCTGATGGAC TCCGCTGTCC CACCCATTGC TGATGAGCCC 
TGGTTCACAA AGACTCGGGT CCGGTACAGG CTGACAGCCA TCGAAGTGGA CCGTTCGGCA 
GGGCCGTACC AAAACTACAC AGTCATCTTT GTTGGCTCTG AGGCCGGCGT GGTGCTTAAA 
GTTTTGGCAA AGACCAGTCC TTTCTCTTTG AACGACAGTG TATTACTCGA AGAGATCGAA 
GCTTATAACC CAGCCAAGTG CAGCGCCGAG AGTGAGGAGG ACAGGAAGGT CGTCTCGTTA 
CAGCTGGACA GGGATCACCA TGCTTTATAC GTGGCCTTCT CCAGCTGCGT GGTCCGCATC 
CCCCTCAGCC GCTGTGAGCG CTATGGCTCC TGTAAAAAGT CTTGCATTGC ATCACGAGAC 
CCGTACTGTG GTTGGTTAAG CCAGGGAGTG TGTGAGAGAG TGACCTTAGG GATGCTTGCT 
GGAGGATATG AGCAGGACAC CGAGTATGGC AACACGGCCC ACCTAGGGGA CTGCCACGAA 
AGTTTGCCTA CTTCAACTAC ACCAGATTAC AAAATATTTG GCGGTCCAAC ATCTGACATG 
GAGGTACCCT CATCTTCTGT TACCACTGTG GCAAGTAGCC CAGAAATTAC ATCTAAAGTG 
ATTGATACCT GGAGACCTAA ACTGACGAGC TCCCGGAAAT TTGTAGTTCA AGATGACCCA 
AACACTTCCG ATTTTACTGA TACTATATCA GGTATCCCAA AGGGTGTACG GTGGGAAGTC 
CAGTCTGGAG ATTCCAACCA GATGGTCCAC ATGAATGTCC TCATCACCTG CGTGTTTGCA 
GCTTTTGTCT TGGGCGCGTT CATCGCAGGA GTGGCTGTGT ACTGCTATCG TGACATGTTT 
GTTCGGAAGA ACAGAAAGAT CCATAAAGAT GCAGAATCGG CCCAGTCATG CACAGATTCC 
AGTGGAAGCT TTGCCAAGCT GAATGGTCTC TTTGACAGCC CCGTCAAGGA GTACCAGCAG 
AACATCGATT CACCCAAACT GTACAGCAAC CTGCTGACCA GTCGGAAGGA GCTGCCTCCC 
AACACGGATC CAAAGTCCAT GGCCATGGAC CATCGAGGCC AGCCTCCAGA GCTGGCTGCT 
CTCCCCACGC CAGAGTCTAC ACCTGTACTC CACCAGAAGA CCCTGCAGGC CATGAAGAGC 
CACTCCGATA AGGCCCATGG CCATGGTGCT TCAAGGAAGG AACACCCCCA GTTTTTTCCT 
TCTAGTCCTC CACCCCATTC CCCGTTAAGT CACGGGCATA TTCCCAGTGC CATCGTTCTT 
CCAAACGCCA CTCATGACTA CAACACATCC TTCTCAAACT CTAACGCTCA CAAAGCCGAA 
AAGAAGCTTC AGAACGTTGA TCACCCTCTC ACAAAGTCAT CCAGTAAGAG GGAACACCGG 
CGCTCTGTGG ACTCCAGAAA CACCCTCAAT GATCTCTTGA AGCATCTCAA TGACCCAAAC 
AGTAACGCCA AAGCCATCAT GGGAGAAATC CACATGGCCC ATCAGACCCT CATGCTGGAC 
CCAGTGGGAC CAATGTCTGA GGTCCCACCC AAGGTTCCTA ACCGGGAGGC ATCCCTATAC 
TCCCCCCCCT CAACACTCCC CAGAAATAGT CCAACCAAGA GAGTAGATGT CCCCACCACT 
CCTGGGGTCC CAATGACTTC TCTGGAAAGA CAAAGGGGTT ATCACAAAAA CTCCTCCCAG 
AGGCACTCTA TATCTGCCGT GCCTAAAAAC TTAAACTCAC CAAACGGTGT TTTGTTATCT 
AGACAGCCGA GTATGAACCG TGGAGGATAT ATGCCCACCC CAACAGGGGC GAAGGTGGAC 
TATATTCAGG GGACACCGGT GAGTGTCCAT CTGCAGCCCT CCCTCTCCAG ACAGAGCAGC 
TACACCAGTA ATGGTACCCT ACCCAGGACG GGACTAAAGA GGACACCATC CTTAAAACCT 
GATGTGCCAC CAAAGCCTTC CTTTGTTCCT CAAACCACAT CTGTCAGACC ACTGAACAAA 
TACACTTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_008762172.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760394.1 Rattus norvegicus semaphorin 6D (Sema6d), transcript variant X2, mRNA.

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CloneID	ORa46988
Clone ID Related Accession (Same CDS sequence)	XM_008762172.2
Accession Version	XM_008762172.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	semaphorin-6D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762172.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCAA AGCATCAATA TGCTCACCAG GTGCTTCAGG GAGGCCTGGC TCGGCGCTGC 
CTTTTGTCTG TTCTTGGAGA TAACCTGTCT ACCACTGCCG GGTCTCTTGA TAAATACGGA 
GCTGTAGAGA GAGGTGGGCA AAAATTTGGA CTGGCTACGA GCCAGAACCC AGTTTCACCA 
CAACATAAAT GTCTTTCCAT GGTGCTTTTG TCTTCCAGGA AGCTAAGTGG GACTTCTGAG 
GAGGGAGCTC AGATACCTTC TCGGCCAACC ATGAGGTTCT TTCTGCTGTG GTTCTGTGTG 
CTGTTCCTTC TGGTCTCCAG GTTACGGGCC GTCAGCTTCC CTGAGGACGA TGAGCCCCTT 
AACACGGTTG ACTACCACTA TTCAAGGCAA TATCCGGTTT TTAGAGGACG CCCTTCAGGC 
AACGAATCGC AGCATAGGCT GGACTTTCAG CTGATGTTGA AAATTCGAGA CACACTTTAT 
ATTGCTGGCA GGGATCAAGT CTATACAGTG AACTTAAATG ACATCCCCCA AACAGAGGTG 
ATCCCGAGCA AGAAGCTGAC ATGGAGGTCC AGACAGCAGG ATCGAGAAAA TTGTGCTATG 
AAAGGCAAGC ATAAAGATGA ATGTCACAAC TTCATCAAAG TCTTTGTCCC AAGAAATGAT 
GAGATGGTTT TTGTCTGTGG CACCAACGCT TTCAACCCGA TGTGCAGATA CTATAGGTTG 
AGTACCTTAG AGTATGATGG GGAAGAAATT AGTGGCCTGG CACGGTGCCC GTTTGATGCC 
CGACAAACCA ATGTTGCCCT CTTTGCTGAT GGGAAACTGT ATTCTGCCAC AGTGGCTGAT 
TTCCTGGCCA GTGATGCTGT CATTTACAGA AGCATGGGTG ATGGATCGGC CCTCCGCACA 
ATAAAATACG ACTCCAAATG GATCAAAGAA CCACACTTTC TTCACGCCAT AGAATATGGA 
AACTATGTCT ATTTCTTCTT CAGAGAAATC GCCGTGGAAC ATAATAACTT AGGCAAGGCT 
GTGTACTCCC GAGTGGCCCG CATTTGTAAA AACGACATGG GTGGCTCACA GCGGGTCCTG 
GAGAAACACT GGACTTCCTT CCTGAAGGCT CGGCTTAACT GCTCAGTCCC TGGAGATTCC 
TTTTTCTACT TCGATGTTCT GCAGTCCATC ACAGACATCA TCCAAATCAA TGGCATCCCC 
ACCGTGATCG GGGTCTTCAC CACGCAGCTC AACAGCATTC CTGGCTCTGC AGTCTGTGCC 
TTTAGCATGG ACGACATTGA GAAAGTGTTC AAAGGGCGGT TCAAAGAGCA GAAAACCCCA 
GACTCTGTTT GGACAGCGGT TCCTGAAGAC AAAGTACCAA AACCAAGGCC TGGCTGTTGT 
GCCAAACATG GCCTCGCGGA AGCTTACAAA ACCTCCATCG ACTTTCCAGA TGATACCCTG 
TCTTTCATCA AGTCCCACCC GCTGATGGAC TCCGCTGTCC CACCCATTGC TGATGAGCCC 
TGGTTCACAA AGACTCGGGT CCGGTACAGG CTGACAGCCA TCGAAGTGGA CCGTTCGGCA 
GGGCCGTACC AAAACTACAC AGTCATCTTT GTTGGCTCTG AGGCCGGCGT GGTGCTTAAA 
GTTTTGGCAA AGACCAGTCC TTTCTCTTTG AACGACAGTG TATTACTCGA AGAGATCGAA 
GCTTATAACC CAGCCAAGTG CAGCGCCGAG AGTGAGGAGG ACAGGAAGGT CGTCTCGTTA 
CAGCTGGACA GGGATCACCA TGCTTTATAC GTGGCCTTCT CCAGCTGCGT GGTCCGCATC 
CCCCTCAGCC GCTGTGAGCG CTATGGCTCC TGTAAAAAGT CTTGCATTGC ATCACGAGAC 
CCGTACTGTG GTTGGTTAAG CCAGGGAGTG TGTGAGAGAG TGACCTTAGG GATGCTTGCT 
GGAGGATATG AGCAGGACAC CGAGTATGGC AACACGGCCC ACCTAGGGGA CTGCCACGAA 
AGTTTGCCTA CTTCAACTAC ACCAGATTAC AAAATATTTG GCGGTCCAAC ATCTGACATG 
GAGGTACCCT CATCTTCTGT TACCACTGTG GCAAGTAGCC CAGAAATTAC ATCTAAAGTG 
ATTGATACCT GGAGACCTAA ACTGACGAGC TCCCGGAAAT TTGTAGTTCA AGATGACCCA 
AACACTTCCG ATTTTACTGA TACTATATCA GGTGTACGGT GGGAAGTCCA GTCTGGAGAT 
TCCAACCAGA TGGTCCACAT GAATGTCCTC ATCACCTGCG TGTTTGCAGC TTTTGTCTTG 
GGCGCGTTCA TCGCAGGAGT GGCTGTGTAC TGCTATCGTG ACATGTTTGT TCGGAAGAAC 
AGAAAGATCC ATAAAGATGC AGAATCGGCC CAGTCATGCA CAGATTCCAG TGGAAGCTTT 
GCCAAGCTGA ATGGTCTCTT TGACAGCCCC GTCAAGGAGT ACCAGCAGAA CATCGATTCA 
CCCAAACTGT ACAGCAACCT GCTGACCAGT CGGAAGGAGC TGCCTCCCAA CACGGATCCA 
AAGTCCATGG CCATGGACCA TCGAGGCCAG CCTCCAGAGC TGGCTGCTCT CCCCACGCCA 
GAGTCTACAC CTGTACTCCA CCAGAAGACC CTGCAGGCCA TGAAGAGCCA CTCCGATAAG 
GCCCATGGCC ATGGTGCTTC AAGGAAGGAA CACCCCCAGT TTTTTCCTTC TAGTCCTCCA 
CCCCATTCCC CGTTAAGTCA CGGGCATATT CCCAGTGCCA TCGTTCTTCC AAACGCCACT 
CATGACTACA ACACATCCTT CTCAAACTCT AACGCTCACA AAGCCGAAAA GAAGCTTCAG 
AACGTTGATC ACCCTCTCAC AAAGTCATCC AGTAAGAGGG AACACCGGCG CTCTGTGGAC 
TCCAGAAACA CCCTCAATGA TCTCTTGAAG CATCTCAATG ACCCAAACAG TAACGCCAAA 
GCCATCATGG GAGAAATCCA CATGGCCCAT CAGACCCTCA TGCTGGACCC AGTGGGACCA 
ATGTCTGAGG TCCCACCCAA GGTTCCTAAC CGGGAGGCAT CCCTATACTC CCCCCCCTCA 
ACACTCCCCA GAAATAGTCC AACCAAGAGA GTAGATGTCC CCACCACTCC TGGGGTCCCA 
ATGACTTCTC TGGAAAGACA AAGGGGTTAT CACAAAAACT CCTCCCAGAG GCACTCTATA 
TCTGCCGTGC CTAAAAACTT AAACTCACCA AACGGTGTTT TGTTATCTAG ACAGCCGAGT 
ATGAACCGTG GAGGATATAT GCCCACCCCA ACAGGGGCGA AGGTGGACTA TATTCAGGGG 
ACACCGGTGA GTGTCCATCT GCAGCCCTCC CTCTCCAGAC AGAGCAGCTA CACCAGTAAT 
GGTACCCTAC CCAGGACGGG ACTAAAGAGG ACACCATCCT TAAAACCTGA TGTGCCACCA 
AAGCCTTCCT TTGTTCCTCA AACCACATCT GTCAGACCAC TGAACAAATA CACTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760394.1
CDS	3589..7068
Translation	MCPKHQYAHQVLQGGLARRCLLSVLGDNLSTTAGSLDKYGAVERGGQKFGLATSQNPVSPQHKCLSMVLLSSRKLSGTSEEGAQIPSRPTMRFFLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNDIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLSTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVIGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLSFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDRDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLAGGYEQDTEYGNTAHLGDCHESLPTSTTPDYKIFGGPTSDMEVPSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGVRWEVQSGDSNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDPKSMAMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSDKAHGHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNVDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNAKAIMGEIHMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_008762172.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus semaphorin 6D (Sema6d), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762172.2

ORF Insert Sequence:

ATGTGCCCAA AGCATCAATA TGCTCACCAG GTGCTTCAGG GAGGCCTGGC TCGGCGCTGC 
CTTTTGTCTG TTCTTGGAGA TAACCTGTCT ACCACTGCCG GGTCTCTTGA TAAATACGGA 
GCTGTAGAGA GAGGTGGGCA AAAATTTGGA CTGGCTACGA GCCAGAACCC AGTTTCACCA 
CAACATAAAT GTCTTTCCAT GGTGCTTTTG TCTTCCAGGA AGCTAAGTGG GACTTCTGAG 
GAGGGAGCTC AGATACCTTC TCGGCCAACC ATGAGGTTCT TTCTGCTGTG GTTCTGTGTG 
CTGTTCCTTC TGGTCTCCAG GTTACGGGCC GTCAGCTTCC CTGAGGACGA TGAGCCCCTT 
AACACGGTTG ACTACCACTA TTCAAGGCAA TATCCGGTTT TTAGAGGACG CCCTTCAGGC 
AACGAATCGC AGCATAGGCT GGACTTTCAG CTGATGTTGA AAATTCGAGA CACACTTTAT 
ATTGCTGGCA GGGATCAAGT CTATACAGTG AACTTAAATG ACATCCCCCA AACAGAGGTG 
ATCCCGAGCA AGAAGCTGAC ATGGAGGTCC AGACAGCAGG ATCGAGAAAA TTGTGCTATG 
AAAGGCAAGC ATAAAGATGA ATGTCACAAC TTCATCAAAG TCTTTGTCCC AAGAAATGAT 
GAGATGGTTT TTGTCTGTGG CACCAACGCT TTCAACCCGA TGTGCAGATA CTATAGGTTG 
AGTACCTTAG AGTATGATGG GGAAGAAATT AGTGGCCTGG CACGGTGCCC GTTTGATGCC 
CGACAAACCA ATGTTGCCCT CTTTGCTGAT GGGAAACTGT ATTCTGCCAC AGTGGCTGAT 
TTCCTGGCCA GTGATGCTGT CATTTACAGA AGCATGGGTG ATGGATCGGC CCTCCGCACA 
ATAAAATACG ACTCCAAATG GATCAAAGAA CCACACTTTC TTCACGCCAT AGAATATGGA 
AACTATGTCT ATTTCTTCTT CAGAGAAATC GCCGTGGAAC ATAATAACTT AGGCAAGGCT 
GTGTACTCCC GAGTGGCCCG CATTTGTAAA AACGACATGG GTGGCTCACA GCGGGTCCTG 
GAGAAACACT GGACTTCCTT CCTGAAGGCT CGGCTTAACT GCTCAGTCCC TGGAGATTCC 
TTTTTCTACT TCGATGTTCT GCAGTCCATC ACAGACATCA TCCAAATCAA TGGCATCCCC 
ACCGTGATCG GGGTCTTCAC CACGCAGCTC AACAGCATTC CTGGCTCTGC AGTCTGTGCC 
TTTAGCATGG ACGACATTGA GAAAGTGTTC AAAGGGCGGT TCAAAGAGCA GAAAACCCCA 
GACTCTGTTT GGACAGCGGT TCCTGAAGAC AAAGTACCAA AACCAAGGCC TGGCTGTTGT 
GCCAAACATG GCCTCGCGGA AGCTTACAAA ACCTCCATCG ACTTTCCAGA TGATACCCTG 
TCTTTCATCA AGTCCCACCC GCTGATGGAC TCCGCTGTCC CACCCATTGC TGATGAGCCC 
TGGTTCACAA AGACTCGGGT CCGGTACAGG CTGACAGCCA TCGAAGTGGA CCGTTCGGCA 
GGGCCGTACC AAAACTACAC AGTCATCTTT GTTGGCTCTG AGGCCGGCGT GGTGCTTAAA 
GTTTTGGCAA AGACCAGTCC TTTCTCTTTG AACGACAGTG TATTACTCGA AGAGATCGAA 
GCTTATAACC CAGCCAAGTG CAGCGCCGAG AGTGAGGAGG ACAGGAAGGT CGTCTCGTTA 
CAGCTGGACA GGGATCACCA TGCTTTATAC GTGGCCTTCT CCAGCTGCGT GGTCCGCATC 
CCCCTCAGCC GCTGTGAGCG CTATGGCTCC TGTAAAAAGT CTTGCATTGC ATCACGAGAC 
CCGTACTGTG GTTGGTTAAG CCAGGGAGTG TGTGAGAGAG TGACCTTAGG GATGCTTGCT 
GGAGGATATG AGCAGGACAC CGAGTATGGC AACACGGCCC ACCTAGGGGA CTGCCACGAA 
AGTTTGCCTA CTTCAACTAC ACCAGATTAC AAAATATTTG GCGGTCCAAC ATCTGACATG 
GAGGTACCCT CATCTTCTGT TACCACTGTG GCAAGTAGCC CAGAAATTAC ATCTAAAGTG 
ATTGATACCT GGAGACCTAA ACTGACGAGC TCCCGGAAAT TTGTAGTTCA AGATGACCCA 
AACACTTCCG ATTTTACTGA TACTATATCA GGTGTACGGT GGGAAGTCCA GTCTGGAGAT 
TCCAACCAGA TGGTCCACAT GAATGTCCTC ATCACCTGCG TGTTTGCAGC TTTTGTCTTG 
GGCGCGTTCA TCGCAGGAGT GGCTGTGTAC TGCTATCGTG ACATGTTTGT TCGGAAGAAC 
AGAAAGATCC ATAAAGATGC AGAATCGGCC CAGTCATGCA CAGATTCCAG TGGAAGCTTT 
GCCAAGCTGA ATGGTCTCTT TGACAGCCCC GTCAAGGAGT ACCAGCAGAA CATCGATTCA 
CCCAAACTGT ACAGCAACCT GCTGACCAGT CGGAAGGAGC TGCCTCCCAA CACGGATCCA 
AAGTCCATGG CCATGGACCA TCGAGGCCAG CCTCCAGAGC TGGCTGCTCT CCCCACGCCA 
GAGTCTACAC CTGTACTCCA CCAGAAGACC CTGCAGGCCA TGAAGAGCCA CTCCGATAAG 
GCCCATGGCC ATGGTGCTTC AAGGAAGGAA CACCCCCAGT TTTTTCCTTC TAGTCCTCCA 
CCCCATTCCC CGTTAAGTCA CGGGCATATT CCCAGTGCCA TCGTTCTTCC AAACGCCACT 
CATGACTACA ACACATCCTT CTCAAACTCT AACGCTCACA AAGCCGAAAA GAAGCTTCAG 
AACGTTGATC ACCCTCTCAC AAAGTCATCC AGTAAGAGGG AACACCGGCG CTCTGTGGAC 
TCCAGAAACA CCCTCAATGA TCTCTTGAAG CATCTCAATG ACCCAAACAG TAACGCCAAA 
GCCATCATGG GAGAAATCCA CATGGCCCAT CAGACCCTCA TGCTGGACCC AGTGGGACCA 
ATGTCTGAGG TCCCACCCAA GGTTCCTAAC CGGGAGGCAT CCCTATACTC CCCCCCCTCA 
ACACTCCCCA GAAATAGTCC AACCAAGAGA GTAGATGTCC CCACCACTCC TGGGGTCCCA 
ATGACTTCTC TGGAAAGACA AAGGGGTTAT CACAAAAACT CCTCCCAGAG GCACTCTATA 
TCTGCCGTGC CTAAAAACTT AAACTCACCA AACGGTGTTT TGTTATCTAG ACAGCCGAGT 
ATGAACCGTG GAGGATATAT GCCCACCCCA ACAGGGGCGA AGGTGGACTA TATTCAGGGG 
ACACCGGTGA GTGTCCATCT GCAGCCCTCC CTCTCCAGAC AGAGCAGCTA CACCAGTAAT 
GGTACCCTAC CCAGGACGGG ACTAAAGAGG ACACCATCCT TAAAACCTGA TGTGCCACCA 
AAGCCTTCCT TTGTTCCTCA AACCACATCT GTCAGACCAC TGAACAAATA CACTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_008762173.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760395.1 Rattus norvegicus semaphorin 6D (Sema6d), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa46989
Clone ID Related Accession (Same CDS sequence)	XM_008762173.2
Accession Version	XM_008762173.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	semaphorin-6D isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762173.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCAA AGCATCAATA TGCTCACCAG GTGCTTCAGG GAGGCCTGGC TCGGCGCTGC 
CTTTTGTCTG TTCTTGGAGA TAACCTGTCT ACCACTGCCG GGTCTCTTGA TAAATACGGA 
GCTGTAGAGA GAGGTGGGCA AAAATTTGGA CTGGCTACGA GCCAGAACCC AGTTTCACCA 
CAACATAAAT GTCTTTCCAT GGTGCTTTTG TCTTCCAGGA AGCTAAGTGG GACTTCTGAG 
GAGGGAGCTC AGATACCTTC TCGGCCAACC ATGAGGTTCT TTCTGCTGTG GTTCTGTGTG 
CTGTTCCTTC TGGTCTCCAG GTTACGGGCC GTCAGCTTCC CTGAGGACGA TGAGCCCCTT 
AACACGGTTG ACTACCACTA TTCAAGGCAA TATCCGGTTT TTAGAGGACG CCCTTCAGGC 
AACGAATCGC AGCATAGGCT GGACTTTCAG CTGATGTTGA AAATTCGAGA CACACTTTAT 
ATTGCTGGCA GGGATCAAGT CTATACAGTG AACTTAAATG ACATCCCCCA AACAGAGGTG 
ATCCCGAGCA AGAAGCTGAC ATGGAGGTCC AGACAGCAGG ATCGAGAAAA TTGTGCTATG 
AAAGGCAAGC ATAAAGATGA ATGTCACAAC TTCATCAAAG TCTTTGTCCC AAGAAATGAT 
GAGATGGTTT TTGTCTGTGG CACCAACGCT TTCAACCCGA TGTGCAGATA CTATAGGTTG 
AGTACCTTAG AGTATGATGG GGAAGAAATT AGTGGCCTGG CACGGTGCCC GTTTGATGCC 
CGACAAACCA ATGTTGCCCT CTTTGCTGAT GGGAAACTGT ATTCTGCCAC AGTGGCTGAT 
TTCCTGGCCA GTGATGCTGT CATTTACAGA AGCATGGGTG ATGGATCGGC CCTCCGCACA 
ATAAAATACG ACTCCAAATG GATCAAAGAA CCACACTTTC TTCACGCCAT AGAATATGGA 
AACTATGTCT ATTTCTTCTT CAGAGAAATC GCCGTGGAAC ATAATAACTT AGGCAAGGCT 
GTGTACTCCC GAGTGGCCCG CATTTGTAAA AACGACATGG GTGGCTCACA GCGGGTCCTG 
GAGAAACACT GGACTTCCTT CCTGAAGGCT CGGCTTAACT GCTCAGTCCC TGGAGATTCC 
TTTTTCTACT TCGATGTTCT GCAGTCCATC ACAGACATCA TCCAAATCAA TGGCATCCCC 
ACCGTGATCG GGGTCTTCAC CACGCAGCTC AACAGCATTC CTGGCTCTGC AGTCTGTGCC 
TTTAGCATGG ACGACATTGA GAAAGTGTTC AAAGGGCGGT TCAAAGAGCA GAAAACCCCA 
GACTCTGTTT GGACAGCGGT TCCTGAAGAC AAAGTACCAA AACCAAGGCC TGGCTGTTGT 
GCCAAACATG GCCTCGCGGA AGCTTACAAA ACCTCCATCG ACTTTCCAGA TGATACCCTG 
TCTTTCATCA AGTCCCACCC GCTGATGGAC TCCGCTGTCC CACCCATTGC TGATGAGCCC 
TGGTTCACAA AGACTCGGGT CCGGTACAGG CTGACAGCCA TCGAAGTGGA CCGTTCGGCA 
GGGCCGTACC AAAACTACAC AGTCATCTTT GTTGGCTCTG AGGCCGGCGT GGTGCTTAAA 
GTTTTGGCAA AGACCAGTCC TTTCTCTTTG AACGACAGTG TATTACTCGA AGAGATCGAA 
GCTTATAACC CAGCCAAGTG CAGCGCCGAG AGTGAGGAGG ACAGGAAGGT CGTCTCGTTA 
CAGCTGGACA GGGATCACCA TGCTTTATAC GTGGCCTTCT CCAGCTGCGT GGTCCGCATC 
CCCCTCAGCC GCTGTGAGCG CTATGGCTCC TGTAAAAAGT CTTGCATTGC ATCACGAGAC 
CCGTACTGTG GTTGGTTAAG CCAGGGAGTG TGTGAGAGAG TGACCTTAGG GATGCTTGCT 
GGAGGATATG AGCAGGACAC CGAGTATGGC AACACGGCCC ACCTAGGGGA CTGCCACGAC 
ATGGAGGTAC CCTCATCTTC TGTTACCACT GTGGCAAGTA GCCCAGAAAT TACATCTAAA 
GTGATTGATA CCTGGAGACC TAAACTGACG AGCTCCCGGA AATTTGTAGT TCAAGATGAC 
CCAAACACTT CCGATTTTAC TGATACTATA TCAGGTATCC CAAAGGGTGT ACGGTGGGAA 
GTCCAGTCTG GAGATTCCAA CCAGATGGTC CACATGAATG TCCTCATCAC CTGCGTGTTT 
GCAGCTTTTG TCTTGGGCGC GTTCATCGCA GGAGTGGCTG TGTACTGCTA TCGTGACATG 
TTTGTTCGGA AGAACAGAAA GATCCATAAA GATGCAGAAT CGGCCCAGTC ATGCACAGAT 
TCCAGTGGAA GCTTTGCCAA GCTGAATGGT CTCTTTGACA GCCCCGTCAA GGAGTACCAG 
CAGAACATCG ATTCACCCAA ACTGTACAGC AACCTGCTGA CCAGTCGGAA GGAGCTGCCT 
CCCAACACGG ATCCAAAGTC CATGGCCATG GACCATCGAG GCCAGCCTCC AGAGCTGGCT 
GCTCTCCCCA CGCCAGAGTC TACACCTGTA CTCCACCAGA AGACCCTGCA GGCCATGAAG 
AGCCACTCCG ATAAGGCCCA TGGCCATGGT GCTTCAAGGA AGGAACACCC CCAGTTTTTT 
CCTTCTAGTC CTCCACCCCA TTCCCCGTTA AGTCACGGGC ATATTCCCAG TGCCATCGTT 
CTTCCAAACG CCACTCATGA CTACAACACA TCCTTCTCAA ACTCTAACGC TCACAAAGCC 
GAAAAGAAGC TTCAGAACGT TGATCACCCT CTCACAAAGT CATCCAGTAA GAGGGAACAC 
CGGCGCTCTG TGGACTCCAG AAACACCCTC AATGATCTCT TGAAGCATCT CAATGACCCA 
AACAGTAACG CCAAAGCCAT CATGGGAGAA ATCCACATGG CCCATCAGAC CCTCATGCTG 
GACCCAGTGG GACCAATGTC TGAGGTCCCA CCCAAGGTTC CTAACCGGGA GGCATCCCTA 
TACTCCCCCC CCTCAACACT CCCCAGAAAT AGTCCAACCA AGAGAGTAGA TGTCCCCACC 
ACTCCTGGGG TCCCAATGAC TTCTCTGGAA AGACAAAGGG GTTATCACAA AAACTCCTCC 
CAGAGGCACT CTATATCTGC CGTGCCTAAA AACTTAAACT CACCAAACGG TGTTTTGTTA 
TCTAGACAGC CGAGTATGAA CCGTGGAGGA TATATGCCCA CCCCAACAGG GGCGAAGGTG 
GACTATATTC AGGGGACACC GGTGAGTGTC CATCTGCAGC CCTCCCTCTC CAGACAGAGC 
AGCTACACCA GTAATGGTAC CCTACCCAGG ACGGGACTAA AGAGGACACC ATCCTTAAAA 
CCTGATGTGC CACCAAAGCC TTCCTTTGTT CCTCAAACCA CATCTGTCAG ACCACTGAAC 
AAATACACTT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760395.1
CDS	3589..7023
Translation	MCPKHQYAHQVLQGGLARRCLLSVLGDNLSTTAGSLDKYGAVERGGQKFGLATSQNPVSPQHKCLSMVLLSSRKLSGTSEEGAQIPSRPTMRFFLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNDIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLSTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVIGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLSFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDRDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLAGGYEQDTEYGNTAHLGDCHDMEVPSSSVTTVASSPEITSKVIDTWRPKLTSSRKFVVQDDPNTSDFTDTISGIPKGVRWEVQSGDSNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDPKSMAMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSDKAHGHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNVDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNAKAIMGEIHMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_008762173.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus semaphorin 6D (Sema6d), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762173.2

ORF Insert Sequence:

ATGTGCCCAA AGCATCAATA TGCTCACCAG GTGCTTCAGG GAGGCCTGGC TCGGCGCTGC 
CTTTTGTCTG TTCTTGGAGA TAACCTGTCT ACCACTGCCG GGTCTCTTGA TAAATACGGA 
GCTGTAGAGA GAGGTGGGCA AAAATTTGGA CTGGCTACGA GCCAGAACCC AGTTTCACCA 
CAACATAAAT GTCTTTCCAT GGTGCTTTTG TCTTCCAGGA AGCTAAGTGG GACTTCTGAG 
GAGGGAGCTC AGATACCTTC TCGGCCAACC ATGAGGTTCT TTCTGCTGTG GTTCTGTGTG 
CTGTTCCTTC TGGTCTCCAG GTTACGGGCC GTCAGCTTCC CTGAGGACGA TGAGCCCCTT 
AACACGGTTG ACTACCACTA TTCAAGGCAA TATCCGGTTT TTAGAGGACG CCCTTCAGGC 
AACGAATCGC AGCATAGGCT GGACTTTCAG CTGATGTTGA AAATTCGAGA CACACTTTAT 
ATTGCTGGCA GGGATCAAGT CTATACAGTG AACTTAAATG ACATCCCCCA AACAGAGGTG 
ATCCCGAGCA AGAAGCTGAC ATGGAGGTCC AGACAGCAGG ATCGAGAAAA TTGTGCTATG 
AAAGGCAAGC ATAAAGATGA ATGTCACAAC TTCATCAAAG TCTTTGTCCC AAGAAATGAT 
GAGATGGTTT TTGTCTGTGG CACCAACGCT TTCAACCCGA TGTGCAGATA CTATAGGTTG 
AGTACCTTAG AGTATGATGG GGAAGAAATT AGTGGCCTGG CACGGTGCCC GTTTGATGCC 
CGACAAACCA ATGTTGCCCT CTTTGCTGAT GGGAAACTGT ATTCTGCCAC AGTGGCTGAT 
TTCCTGGCCA GTGATGCTGT CATTTACAGA AGCATGGGTG ATGGATCGGC CCTCCGCACA 
ATAAAATACG ACTCCAAATG GATCAAAGAA CCACACTTTC TTCACGCCAT AGAATATGGA 
AACTATGTCT ATTTCTTCTT CAGAGAAATC GCCGTGGAAC ATAATAACTT AGGCAAGGCT 
GTGTACTCCC GAGTGGCCCG CATTTGTAAA AACGACATGG GTGGCTCACA GCGGGTCCTG 
GAGAAACACT GGACTTCCTT CCTGAAGGCT CGGCTTAACT GCTCAGTCCC TGGAGATTCC 
TTTTTCTACT TCGATGTTCT GCAGTCCATC ACAGACATCA TCCAAATCAA TGGCATCCCC 
ACCGTGATCG GGGTCTTCAC CACGCAGCTC AACAGCATTC CTGGCTCTGC AGTCTGTGCC 
TTTAGCATGG ACGACATTGA GAAAGTGTTC AAAGGGCGGT TCAAAGAGCA GAAAACCCCA 
GACTCTGTTT GGACAGCGGT TCCTGAAGAC AAAGTACCAA AACCAAGGCC TGGCTGTTGT 
GCCAAACATG GCCTCGCGGA AGCTTACAAA ACCTCCATCG ACTTTCCAGA TGATACCCTG 
TCTTTCATCA AGTCCCACCC GCTGATGGAC TCCGCTGTCC CACCCATTGC TGATGAGCCC 
TGGTTCACAA AGACTCGGGT CCGGTACAGG CTGACAGCCA TCGAAGTGGA CCGTTCGGCA 
GGGCCGTACC AAAACTACAC AGTCATCTTT GTTGGCTCTG AGGCCGGCGT GGTGCTTAAA 
GTTTTGGCAA AGACCAGTCC TTTCTCTTTG AACGACAGTG TATTACTCGA AGAGATCGAA 
GCTTATAACC CAGCCAAGTG CAGCGCCGAG AGTGAGGAGG ACAGGAAGGT CGTCTCGTTA 
CAGCTGGACA GGGATCACCA TGCTTTATAC GTGGCCTTCT CCAGCTGCGT GGTCCGCATC 
CCCCTCAGCC GCTGTGAGCG CTATGGCTCC TGTAAAAAGT CTTGCATTGC ATCACGAGAC 
CCGTACTGTG GTTGGTTAAG CCAGGGAGTG TGTGAGAGAG TGACCTTAGG GATGCTTGCT 
GGAGGATATG AGCAGGACAC CGAGTATGGC AACACGGCCC ACCTAGGGGA CTGCCACGAC 
ATGGAGGTAC CCTCATCTTC TGTTACCACT GTGGCAAGTA GCCCAGAAAT TACATCTAAA 
GTGATTGATA CCTGGAGACC TAAACTGACG AGCTCCCGGA AATTTGTAGT TCAAGATGAC 
CCAAACACTT CCGATTTTAC TGATACTATA TCAGGTATCC CAAAGGGTGT ACGGTGGGAA 
GTCCAGTCTG GAGATTCCAA CCAGATGGTC CACATGAATG TCCTCATCAC CTGCGTGTTT 
GCAGCTTTTG TCTTGGGCGC GTTCATCGCA GGAGTGGCTG TGTACTGCTA TCGTGACATG 
TTTGTTCGGA AGAACAGAAA GATCCATAAA GATGCAGAAT CGGCCCAGTC ATGCACAGAT 
TCCAGTGGAA GCTTTGCCAA GCTGAATGGT CTCTTTGACA GCCCCGTCAA GGAGTACCAG 
CAGAACATCG ATTCACCCAA ACTGTACAGC AACCTGCTGA CCAGTCGGAA GGAGCTGCCT 
CCCAACACGG ATCCAAAGTC CATGGCCATG GACCATCGAG GCCAGCCTCC AGAGCTGGCT 
GCTCTCCCCA CGCCAGAGTC TACACCTGTA CTCCACCAGA AGACCCTGCA GGCCATGAAG 
AGCCACTCCG ATAAGGCCCA TGGCCATGGT GCTTCAAGGA AGGAACACCC CCAGTTTTTT 
CCTTCTAGTC CTCCACCCCA TTCCCCGTTA AGTCACGGGC ATATTCCCAG TGCCATCGTT 
CTTCCAAACG CCACTCATGA CTACAACACA TCCTTCTCAA ACTCTAACGC TCACAAAGCC 
GAAAAGAAGC TTCAGAACGT TGATCACCCT CTCACAAAGT CATCCAGTAA GAGGGAACAC 
CGGCGCTCTG TGGACTCCAG AAACACCCTC AATGATCTCT TGAAGCATCT CAATGACCCA 
AACAGTAACG CCAAAGCCAT CATGGGAGAA ATCCACATGG CCCATCAGAC CCTCATGCTG 
GACCCAGTGG GACCAATGTC TGAGGTCCCA CCCAAGGTTC CTAACCGGGA GGCATCCCTA 
TACTCCCCCC CCTCAACACT CCCCAGAAAT AGTCCAACCA AGAGAGTAGA TGTCCCCACC 
ACTCCTGGGG TCCCAATGAC TTCTCTGGAA AGACAAAGGG GTTATCACAA AAACTCCTCC 
CAGAGGCACT CTATATCTGC CGTGCCTAAA AACTTAAACT CACCAAACGG TGTTTTGTTA 
TCTAGACAGC CGAGTATGAA CCGTGGAGGA TATATGCCCA CCCCAACAGG GGCGAAGGTG 
GACTATATTC AGGGGACACC GGTGAGTGTC CATCTGCAGC CCTCCCTCTC CAGACAGAGC 
AGCTACACCA GTAATGGTAC CCTACCCAGG ACGGGACTAA AGAGGACACC ATCCTTAAAA 
CCTGATGTGC CACCAAAGCC TTCCTTTGTT CCTCAAACCA CATCTGTCAG ACCACTGAAC 
AAATACACTT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_008762174.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760396.1 Rattus norvegicus semaphorin 6D (Sema6d), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa46990
Clone ID Related Accession (Same CDS sequence)	XM_008762174.2
Accession Version	XM_008762174.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	semaphorin-6D isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762174.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCAA AGCATCAATA TGCTCACCAG GTGCTTCAGG GAGGCCTGGC TCGGCGCTGC 
CTTTTGTCTG TTCTTGGAGA TAACCTGTCT ACCACTGCCG GGTCTCTTGA TAAATACGGA 
GCTGTAGAGA GAGGTGGGCA AAAATTTGGA CTGGCTACGA GCCAGAACCC AGTTTCACCA 
CAACATAAAT GTCTTTCCAT GGTGCTTTTG TCTTCCAGGA AGCTAAGTGG GACTTCTGAG 
GAGGGAGCTC AGATACCTTC TCGGCCAACC ATGAGGTTCT TTCTGCTGTG GTTCTGTGTG 
CTGTTCCTTC TGGTCTCCAG GTTACGGGCC GTCAGCTTCC CTGAGGACGA TGAGCCCCTT 
AACACGGTTG ACTACCACTA TTCAAGGCAA TATCCGGTTT TTAGAGGACG CCCTTCAGGC 
AACGAATCGC AGCATAGGCT GGACTTTCAG CTGATGTTGA AAATTCGAGA CACACTTTAT 
ATTGCTGGCA GGGATCAAGT CTATACAGTG AACTTAAATG ACATCCCCCA AACAGAGGTG 
ATCCCGAGCA AGAAGCTGAC ATGGAGGTCC AGACAGCAGG ATCGAGAAAA TTGTGCTATG 
AAAGGCAAGC ATAAAGATGA ATGTCACAAC TTCATCAAAG TCTTTGTCCC AAGAAATGAT 
GAGATGGTTT TTGTCTGTGG CACCAACGCT TTCAACCCGA TGTGCAGATA CTATAGGTTG 
AGTACCTTAG AGTATGATGG GGAAGAAATT AGTGGCCTGG CACGGTGCCC GTTTGATGCC 
CGACAAACCA ATGTTGCCCT CTTTGCTGAT GGGAAACTGT ATTCTGCCAC AGTGGCTGAT 
TTCCTGGCCA GTGATGCTGT CATTTACAGA AGCATGGGTG ATGGATCGGC CCTCCGCACA 
ATAAAATACG ACTCCAAATG GATCAAAGAA CCACACTTTC TTCACGCCAT AGAATATGGA 
AACTATGTCT ATTTCTTCTT CAGAGAAATC GCCGTGGAAC ATAATAACTT AGGCAAGGCT 
GTGTACTCCC GAGTGGCCCG CATTTGTAAA AACGACATGG GTGGCTCACA GCGGGTCCTG 
GAGAAACACT GGACTTCCTT CCTGAAGGCT CGGCTTAACT GCTCAGTCCC TGGAGATTCC 
TTTTTCTACT TCGATGTTCT GCAGTCCATC ACAGACATCA TCCAAATCAA TGGCATCCCC 
ACCGTGATCG GGGTCTTCAC CACGCAGCTC AACAGCATTC CTGGCTCTGC AGTCTGTGCC 
TTTAGCATGG ACGACATTGA GAAAGTGTTC AAAGGGCGGT TCAAAGAGCA GAAAACCCCA 
GACTCTGTTT GGACAGCGGT TCCTGAAGAC AAAGTACCAA AACCAAGGCC TGGCTGTTGT 
GCCAAACATG GCCTCGCGGA AGCTTACAAA ACCTCCATCG ACTTTCCAGA TGATACCCTG 
TCTTTCATCA AGTCCCACCC GCTGATGGAC TCCGCTGTCC CACCCATTGC TGATGAGCCC 
TGGTTCACAA AGACTCGGGT CCGGTACAGG CTGACAGCCA TCGAAGTGGA CCGTTCGGCA 
GGGCCGTACC AAAACTACAC AGTCATCTTT GTTGGCTCTG AGGCCGGCGT GGTGCTTAAA 
GTTTTGGCAA AGACCAGTCC TTTCTCTTTG AACGACAGTG TATTACTCGA AGAGATCGAA 
GCTTATAACC CAGCCAAGTG CAGCGCCGAG AGTGAGGAGG ACAGGAAGGT CGTCTCGTTA 
CAGCTGGACA GGGATCACCA TGCTTTATAC GTGGCCTTCT CCAGCTGCGT GGTCCGCATC 
CCCCTCAGCC GCTGTGAGCG CTATGGCTCC TGTAAAAAGT CTTGCATTGC ATCACGAGAC 
CCGTACTGTG GTTGGTTAAG CCAGGGAGTG TGTGAGAGAG TGACCTTAGG GATGCTTGCT 
GGAGGATATG AGCAGGACAC CGAGTATGGC AACACGGCCC ACCTAGGGGA CTGCCACGAA 
AGTTTGCCTA CTTCAACTAC ACCAGATTAC AAAATATTTG GCGGTCCAAC ATCTGGTGTA 
CGGTGGGAAG TCCAGTCTGG AGATTCCAAC CAGATGGTCC ACATGAATGT CCTCATCACC 
TGCGTGTTTG CAGCTTTTGT CTTGGGCGCG TTCATCGCAG GAGTGGCTGT GTACTGCTAT 
CGTGACATGT TTGTTCGGAA GAACAGAAAG ATCCATAAAG ATGCAGAATC GGCCCAGTCA 
TGCACAGATT CCAGTGGAAG CTTTGCCAAG CTGAATGGTC TCTTTGACAG CCCCGTCAAG 
GAGTACCAGC AGAACATCGA TTCACCCAAA CTGTACAGCA ACCTGCTGAC CAGTCGGAAG 
GAGCTGCCTC CCAACACGGA TCCAAAGTCC ATGGCCATGG ACCATCGAGG CCAGCCTCCA 
GAGCTGGCTG CTCTCCCCAC GCCAGAGTCT ACACCTGTAC TCCACCAGAA GACCCTGCAG 
GCCATGAAGA GCCACTCCGA TAAGGCCCAT GGCCATGGTG CTTCAAGGAA GGAACACCCC 
CAGTTTTTTC CTTCTAGTCC TCCACCCCAT TCCCCGTTAA GTCACGGGCA TATTCCCAGT 
GCCATCGTTC TTCCAAACGC CACTCATGAC TACAACACAT CCTTCTCAAA CTCTAACGCT 
CACAAAGCCG AAAAGAAGCT TCAGAACGTT GATCACCCTC TCACAAAGTC ATCCAGTAAG 
AGGGAACACC GGCGCTCTGT GGACTCCAGA AACACCCTCA ATGATCTCTT GAAGCATCTC 
AATGACCCAA ACAGTAACGC CAAAGCCATC ATGGGAGAAA TCCACATGGC CCATCAGACC 
CTCATGCTGG ACCCAGTGGG ACCAATGTCT GAGGTCCCAC CCAAGGTTCC TAACCGGGAG 
GCATCCCTAT ACTCCCCCCC CTCAACACTC CCCAGAAATA GTCCAACCAA GAGAGTAGAT 
GTCCCCACCA CTCCTGGGGT CCCAATGACT TCTCTGGAAA GACAAAGGGG TTATCACAAA 
AACTCCTCCC AGAGGCACTC TATATCTGCC GTGCCTAAAA ACTTAAACTC ACCAAACGGT 
GTTTTGTTAT CTAGACAGCC GAGTATGAAC CGTGGAGGAT ATATGCCCAC CCCAACAGGG 
GCGAAGGTGG ACTATATTCA GGGGACACCG GTGAGTGTCC ATCTGCAGCC CTCCCTCTCC 
AGACAGAGCA GCTACACCAG TAATGGTACC CTACCCAGGA CGGGACTAAA GAGGACACCA 
TCCTTAAAAC CTGATGTGCC ACCAAAGCCT TCCTTTGTTC CTCAAACCAC ATCTGTCAGA 
CCACTGAACA AATACACTTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760396.1
CDS	3589..6912
Translation	MCPKHQYAHQVLQGGLARRCLLSVLGDNLSTTAGSLDKYGAVERGGQKFGLATSQNPVSPQHKCLSMVLLSSRKLSGTSEEGAQIPSRPTMRFFLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNDIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLSTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVIGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLSFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDRDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLAGGYEQDTEYGNTAHLGDCHESLPTSTTPDYKIFGGPTSGVRWEVQSGDSNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDPKSMAMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSDKAHGHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNVDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNAKAIMGEIHMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_008762174.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus semaphorin 6D (Sema6d), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762174.2

ORF Insert Sequence:

ATGTGCCCAA AGCATCAATA TGCTCACCAG GTGCTTCAGG GAGGCCTGGC TCGGCGCTGC 
CTTTTGTCTG TTCTTGGAGA TAACCTGTCT ACCACTGCCG GGTCTCTTGA TAAATACGGA 
GCTGTAGAGA GAGGTGGGCA AAAATTTGGA CTGGCTACGA GCCAGAACCC AGTTTCACCA 
CAACATAAAT GTCTTTCCAT GGTGCTTTTG TCTTCCAGGA AGCTAAGTGG GACTTCTGAG 
GAGGGAGCTC AGATACCTTC TCGGCCAACC ATGAGGTTCT TTCTGCTGTG GTTCTGTGTG 
CTGTTCCTTC TGGTCTCCAG GTTACGGGCC GTCAGCTTCC CTGAGGACGA TGAGCCCCTT 
AACACGGTTG ACTACCACTA TTCAAGGCAA TATCCGGTTT TTAGAGGACG CCCTTCAGGC 
AACGAATCGC AGCATAGGCT GGACTTTCAG CTGATGTTGA AAATTCGAGA CACACTTTAT 
ATTGCTGGCA GGGATCAAGT CTATACAGTG AACTTAAATG ACATCCCCCA AACAGAGGTG 
ATCCCGAGCA AGAAGCTGAC ATGGAGGTCC AGACAGCAGG ATCGAGAAAA TTGTGCTATG 
AAAGGCAAGC ATAAAGATGA ATGTCACAAC TTCATCAAAG TCTTTGTCCC AAGAAATGAT 
GAGATGGTTT TTGTCTGTGG CACCAACGCT TTCAACCCGA TGTGCAGATA CTATAGGTTG 
AGTACCTTAG AGTATGATGG GGAAGAAATT AGTGGCCTGG CACGGTGCCC GTTTGATGCC 
CGACAAACCA ATGTTGCCCT CTTTGCTGAT GGGAAACTGT ATTCTGCCAC AGTGGCTGAT 
TTCCTGGCCA GTGATGCTGT CATTTACAGA AGCATGGGTG ATGGATCGGC CCTCCGCACA 
ATAAAATACG ACTCCAAATG GATCAAAGAA CCACACTTTC TTCACGCCAT AGAATATGGA 
AACTATGTCT ATTTCTTCTT CAGAGAAATC GCCGTGGAAC ATAATAACTT AGGCAAGGCT 
GTGTACTCCC GAGTGGCCCG CATTTGTAAA AACGACATGG GTGGCTCACA GCGGGTCCTG 
GAGAAACACT GGACTTCCTT CCTGAAGGCT CGGCTTAACT GCTCAGTCCC TGGAGATTCC 
TTTTTCTACT TCGATGTTCT GCAGTCCATC ACAGACATCA TCCAAATCAA TGGCATCCCC 
ACCGTGATCG GGGTCTTCAC CACGCAGCTC AACAGCATTC CTGGCTCTGC AGTCTGTGCC 
TTTAGCATGG ACGACATTGA GAAAGTGTTC AAAGGGCGGT TCAAAGAGCA GAAAACCCCA 
GACTCTGTTT GGACAGCGGT TCCTGAAGAC AAAGTACCAA AACCAAGGCC TGGCTGTTGT 
GCCAAACATG GCCTCGCGGA AGCTTACAAA ACCTCCATCG ACTTTCCAGA TGATACCCTG 
TCTTTCATCA AGTCCCACCC GCTGATGGAC TCCGCTGTCC CACCCATTGC TGATGAGCCC 
TGGTTCACAA AGACTCGGGT CCGGTACAGG CTGACAGCCA TCGAAGTGGA CCGTTCGGCA 
GGGCCGTACC AAAACTACAC AGTCATCTTT GTTGGCTCTG AGGCCGGCGT GGTGCTTAAA 
GTTTTGGCAA AGACCAGTCC TTTCTCTTTG AACGACAGTG TATTACTCGA AGAGATCGAA 
GCTTATAACC CAGCCAAGTG CAGCGCCGAG AGTGAGGAGG ACAGGAAGGT CGTCTCGTTA 
CAGCTGGACA GGGATCACCA TGCTTTATAC GTGGCCTTCT CCAGCTGCGT GGTCCGCATC 
CCCCTCAGCC GCTGTGAGCG CTATGGCTCC TGTAAAAAGT CTTGCATTGC ATCACGAGAC 
CCGTACTGTG GTTGGTTAAG CCAGGGAGTG TGTGAGAGAG TGACCTTAGG GATGCTTGCT 
GGAGGATATG AGCAGGACAC CGAGTATGGC AACACGGCCC ACCTAGGGGA CTGCCACGAA 
AGTTTGCCTA CTTCAACTAC ACCAGATTAC AAAATATTTG GCGGTCCAAC ATCTGGTGTA 
CGGTGGGAAG TCCAGTCTGG AGATTCCAAC CAGATGGTCC ACATGAATGT CCTCATCACC 
TGCGTGTTTG CAGCTTTTGT CTTGGGCGCG TTCATCGCAG GAGTGGCTGT GTACTGCTAT 
CGTGACATGT TTGTTCGGAA GAACAGAAAG ATCCATAAAG ATGCAGAATC GGCCCAGTCA 
TGCACAGATT CCAGTGGAAG CTTTGCCAAG CTGAATGGTC TCTTTGACAG CCCCGTCAAG 
GAGTACCAGC AGAACATCGA TTCACCCAAA CTGTACAGCA ACCTGCTGAC CAGTCGGAAG 
GAGCTGCCTC CCAACACGGA TCCAAAGTCC ATGGCCATGG ACCATCGAGG CCAGCCTCCA 
GAGCTGGCTG CTCTCCCCAC GCCAGAGTCT ACACCTGTAC TCCACCAGAA GACCCTGCAG 
GCCATGAAGA GCCACTCCGA TAAGGCCCAT GGCCATGGTG CTTCAAGGAA GGAACACCCC 
CAGTTTTTTC CTTCTAGTCC TCCACCCCAT TCCCCGTTAA GTCACGGGCA TATTCCCAGT 
GCCATCGTTC TTCCAAACGC CACTCATGAC TACAACACAT CCTTCTCAAA CTCTAACGCT 
CACAAAGCCG AAAAGAAGCT TCAGAACGTT GATCACCCTC TCACAAAGTC ATCCAGTAAG 
AGGGAACACC GGCGCTCTGT GGACTCCAGA AACACCCTCA ATGATCTCTT GAAGCATCTC 
AATGACCCAA ACAGTAACGC CAAAGCCATC ATGGGAGAAA TCCACATGGC CCATCAGACC 
CTCATGCTGG ACCCAGTGGG ACCAATGTCT GAGGTCCCAC CCAAGGTTCC TAACCGGGAG 
GCATCCCTAT ACTCCCCCCC CTCAACACTC CCCAGAAATA GTCCAACCAA GAGAGTAGAT 
GTCCCCACCA CTCCTGGGGT CCCAATGACT TCTCTGGAAA GACAAAGGGG TTATCACAAA 
AACTCCTCCC AGAGGCACTC TATATCTGCC GTGCCTAAAA ACTTAAACTC ACCAAACGGT 
GTTTTGTTAT CTAGACAGCC GAGTATGAAC CGTGGAGGAT ATATGCCCAC CCCAACAGGG 
GCGAAGGTGG ACTATATTCA GGGGACACCG GTGAGTGTCC ATCTGCAGCC CTCCCTCTCC 
AGACAGAGCA GCTACACCAG TAATGGTACC CTACCCAGGA CGGGACTAAA GAGGACACCA 
TCCTTAAAAC CTGATGTGCC ACCAAAGCCT TCCTTTGTTC CTCAAACCAC ATCTGTCAGA 
CCACTGAACA AATACACTTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6d ( XM_008762175.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760397.1 Rattus norvegicus semaphorin 6D (Sema6d), transcript variant X7, mRNA.

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CloneID	ORa46991
Clone ID Related Accession (Same CDS sequence)	XM_008762175.2
Accession Version	XM_008762175.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	semaphorin-6D isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762175.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCAA AGCATCAATA TGCTCACCAG GTGCTTCAGG GAGGCCTGGC TCGGCGCTGC 
CTTTTGTCTG TTCTTGGAGA TAACCTGTCT ACCACTGCCG GGTCTCTTGA TAAATACGGA 
GCTGTAGAGA GAGGTGGGCA AAAATTTGGA CTGGCTACGA GCCAGAACCC AGTTTCACCA 
CAACATAAAT GTCTTTCCAT GGTGCTTTTG TCTTCCAGGA AGCTAAGTGG GACTTCTGAG 
GAGGGAGCTC AGATACCTTC TCGGCCAACC ATGAGGTTCT TTCTGCTGTG GTTCTGTGTG 
CTGTTCCTTC TGGTCTCCAG GTTACGGGCC GTCAGCTTCC CTGAGGACGA TGAGCCCCTT 
AACACGGTTG ACTACCACTA TTCAAGGCAA TATCCGGTTT TTAGAGGACG CCCTTCAGGC 
AACGAATCGC AGCATAGGCT GGACTTTCAG CTGATGTTGA AAATTCGAGA CACACTTTAT 
ATTGCTGGCA GGGATCAAGT CTATACAGTG AACTTAAATG ACATCCCCCA AACAGAGGTG 
ATCCCGAGCA AGAAGCTGAC ATGGAGGTCC AGACAGCAGG ATCGAGAAAA TTGTGCTATG 
AAAGGCAAGC ATAAAGATGA ATGTCACAAC TTCATCAAAG TCTTTGTCCC AAGAAATGAT 
GAGATGGTTT TTGTCTGTGG CACCAACGCT TTCAACCCGA TGTGCAGATA CTATAGGTTG 
AGTACCTTAG AGTATGATGG GGAAGAAATT AGTGGCCTGG CACGGTGCCC GTTTGATGCC 
CGACAAACCA ATGTTGCCCT CTTTGCTGAT GGGAAACTGT ATTCTGCCAC AGTGGCTGAT 
TTCCTGGCCA GTGATGCTGT CATTTACAGA AGCATGGGTG ATGGATCGGC CCTCCGCACA 
ATAAAATACG ACTCCAAATG GATCAAAGAA CCACACTTTC TTCACGCCAT AGAATATGGA 
AACTATGTCT ATTTCTTCTT CAGAGAAATC GCCGTGGAAC ATAATAACTT AGGCAAGGCT 
GTGTACTCCC GAGTGGCCCG CATTTGTAAA AACGACATGG GTGGCTCACA GCGGGTCCTG 
GAGAAACACT GGACTTCCTT CCTGAAGGCT CGGCTTAACT GCTCAGTCCC TGGAGATTCC 
TTTTTCTACT TCGATGTTCT GCAGTCCATC ACAGACATCA TCCAAATCAA TGGCATCCCC 
ACCGTGATCG GGGTCTTCAC CACGCAGCTC AACAGCATTC CTGGCTCTGC AGTCTGTGCC 
TTTAGCATGG ACGACATTGA GAAAGTGTTC AAAGGGCGGT TCAAAGAGCA GAAAACCCCA 
GACTCTGTTT GGACAGCGGT TCCTGAAGAC AAAGTACCAA AACCAAGGCC TGGCTGTTGT 
GCCAAACATG GCCTCGCGGA AGCTTACAAA ACCTCCATCG ACTTTCCAGA TGATACCCTG 
TCTTTCATCA AGTCCCACCC GCTGATGGAC TCCGCTGTCC CACCCATTGC TGATGAGCCC 
TGGTTCACAA AGACTCGGGT CCGGTACAGG CTGACAGCCA TCGAAGTGGA CCGTTCGGCA 
GGGCCGTACC AAAACTACAC AGTCATCTTT GTTGGCTCTG AGGCCGGCGT GGTGCTTAAA 
GTTTTGGCAA AGACCAGTCC TTTCTCTTTG AACGACAGTG TATTACTCGA AGAGATCGAA 
GCTTATAACC CAGCCAAGTG CAGCGCCGAG AGTGAGGAGG ACAGGAAGGT CGTCTCGTTA 
CAGCTGGACA GGGATCACCA TGCTTTATAC GTGGCCTTCT CCAGCTGCGT GGTCCGCATC 
CCCCTCAGCC GCTGTGAGCG CTATGGCTCC TGTAAAAAGT CTTGCATTGC ATCACGAGAC 
CCGTACTGTG GTTGGTTAAG CCAGGGAGTG TGTGAGAGAG TGACCTTAGG GATGCTTGCT 
GGAGGATATG AGCAGGACAC CGAGTATGGC AACACGGCCC ACCTAGGGGA CTGCCACGGT 
GTACGGTGGG AAGTCCAGTC TGGAGATTCC AACCAGATGG TCCACATGAA TGTCCTCATC 
ACCTGCGTGT TTGCAGCTTT TGTCTTGGGC GCGTTCATCG CAGGAGTGGC TGTGTACTGC 
TATCGTGACA TGTTTGTTCG GAAGAACAGA AAGATCCATA AAGATGCAGA ATCGGCCCAG 
TCATGCACAG ATTCCAGTGG AAGCTTTGCC AAGCTGAATG GTCTCTTTGA CAGCCCCGTC 
AAGGAGTACC AGCAGAACAT CGATTCACCC AAACTGTACA GCAACCTGCT GACCAGTCGG 
AAGGAGCTGC CTCCCAACAC GGATCCAAAG TCCATGGCCA TGGACCATCG AGGCCAGCCT 
CCAGAGCTGG CTGCTCTCCC CACGCCAGAG TCTACACCTG TACTCCACCA GAAGACCCTG 
CAGGCCATGA AGAGCCACTC CGATAAGGCC CATGGCCATG GTGCTTCAAG GAAGGAACAC 
CCCCAGTTTT TTCCTTCTAG TCCTCCACCC CATTCCCCGT TAAGTCACGG GCATATTCCC 
AGTGCCATCG TTCTTCCAAA CGCCACTCAT GACTACAACA CATCCTTCTC AAACTCTAAC 
GCTCACAAAG CCGAAAAGAA GCTTCAGAAC GTTGATCACC CTCTCACAAA GTCATCCAGT 
AAGAGGGAAC ACCGGCGCTC TGTGGACTCC AGAAACACCC TCAATGATCT CTTGAAGCAT 
CTCAATGACC CAAACAGTAA CGCCAAAGCC ATCATGGGAG AAATCCACAT GGCCCATCAG 
ACCCTCATGC TGGACCCAGT GGGACCAATG TCTGAGGTCC CACCCAAGGT TCCTAACCGG 
GAGGCATCCC TATACTCCCC CCCCTCAACA CTCCCCAGAA ATAGTCCAAC CAAGAGAGTA 
GATGTCCCCA CCACTCCTGG GGTCCCAATG ACTTCTCTGG AAAGACAAAG GGGTTATCAC 
AAAAACTCCT CCCAGAGGCA CTCTATATCT GCCGTGCCTA AAAACTTAAA CTCACCAAAC 
GGTGTTTTGT TATCTAGACA GCCGAGTATG AACCGTGGAG GATATATGCC CACCCCAACA 
GGGGCGAAGG TGGACTATAT TCAGGGGACA CCGGTGAGTG TCCATCTGCA GCCCTCCCTC 
TCCAGACAGA GCAGCTACAC CAGTAATGGT ACCCTACCCA GGACGGGACT AAAGAGGACA 
CCATCCTTAA AACCTGATGT GCCACCAAAG CCTTCCTTTG TTCCTCAAAC CACATCTGTC 
AGACCACTGA ACAAATACAC TTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760397.1
CDS	3588..6854
Translation	MCPKHQYAHQVLQGGLARRCLLSVLGDNLSTTAGSLDKYGAVERGGQKFGLATSQNPVSPQHKCLSMVLLSSRKLSGTSEEGAQIPSRPTMRFFLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNDIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRLSTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVIGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLSFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSLQLDRDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLAGGYEQDTEYGNTAHLGDCHGVRWEVQSGDSNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDPKSMAMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSDKAHGHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNVDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNAKAIMGEIHMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPNGVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_008762175.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus semaphorin 6D (Sema6d), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762175.2

ORF Insert Sequence:

ATGTGCCCAA AGCATCAATA TGCTCACCAG GTGCTTCAGG GAGGCCTGGC TCGGCGCTGC 
CTTTTGTCTG TTCTTGGAGA TAACCTGTCT ACCACTGCCG GGTCTCTTGA TAAATACGGA 
GCTGTAGAGA GAGGTGGGCA AAAATTTGGA CTGGCTACGA GCCAGAACCC AGTTTCACCA 
CAACATAAAT GTCTTTCCAT GGTGCTTTTG TCTTCCAGGA AGCTAAGTGG GACTTCTGAG 
GAGGGAGCTC AGATACCTTC TCGGCCAACC ATGAGGTTCT TTCTGCTGTG GTTCTGTGTG 
CTGTTCCTTC TGGTCTCCAG GTTACGGGCC GTCAGCTTCC CTGAGGACGA TGAGCCCCTT 
AACACGGTTG ACTACCACTA TTCAAGGCAA TATCCGGTTT TTAGAGGACG CCCTTCAGGC 
AACGAATCGC AGCATAGGCT GGACTTTCAG CTGATGTTGA AAATTCGAGA CACACTTTAT 
ATTGCTGGCA GGGATCAAGT CTATACAGTG AACTTAAATG ACATCCCCCA AACAGAGGTG 
ATCCCGAGCA AGAAGCTGAC ATGGAGGTCC AGACAGCAGG ATCGAGAAAA TTGTGCTATG 
AAAGGCAAGC ATAAAGATGA ATGTCACAAC TTCATCAAAG TCTTTGTCCC AAGAAATGAT 
GAGATGGTTT TTGTCTGTGG CACCAACGCT TTCAACCCGA TGTGCAGATA CTATAGGTTG 
AGTACCTTAG AGTATGATGG GGAAGAAATT AGTGGCCTGG CACGGTGCCC GTTTGATGCC 
CGACAAACCA ATGTTGCCCT CTTTGCTGAT GGGAAACTGT ATTCTGCCAC AGTGGCTGAT 
TTCCTGGCCA GTGATGCTGT CATTTACAGA AGCATGGGTG ATGGATCGGC CCTCCGCACA 
ATAAAATACG ACTCCAAATG GATCAAAGAA CCACACTTTC TTCACGCCAT AGAATATGGA 
AACTATGTCT ATTTCTTCTT CAGAGAAATC GCCGTGGAAC ATAATAACTT AGGCAAGGCT 
GTGTACTCCC GAGTGGCCCG CATTTGTAAA AACGACATGG GTGGCTCACA GCGGGTCCTG 
GAGAAACACT GGACTTCCTT CCTGAAGGCT CGGCTTAACT GCTCAGTCCC TGGAGATTCC 
TTTTTCTACT TCGATGTTCT GCAGTCCATC ACAGACATCA TCCAAATCAA TGGCATCCCC 
ACCGTGATCG GGGTCTTCAC CACGCAGCTC AACAGCATTC CTGGCTCTGC AGTCTGTGCC 
TTTAGCATGG ACGACATTGA GAAAGTGTTC AAAGGGCGGT TCAAAGAGCA GAAAACCCCA 
GACTCTGTTT GGACAGCGGT TCCTGAAGAC AAAGTACCAA AACCAAGGCC TGGCTGTTGT 
GCCAAACATG GCCTCGCGGA AGCTTACAAA ACCTCCATCG ACTTTCCAGA TGATACCCTG 
TCTTTCATCA AGTCCCACCC GCTGATGGAC TCCGCTGTCC CACCCATTGC TGATGAGCCC 
TGGTTCACAA AGACTCGGGT CCGGTACAGG CTGACAGCCA TCGAAGTGGA CCGTTCGGCA 
GGGCCGTACC AAAACTACAC AGTCATCTTT GTTGGCTCTG AGGCCGGCGT GGTGCTTAAA 
GTTTTGGCAA AGACCAGTCC TTTCTCTTTG AACGACAGTG TATTACTCGA AGAGATCGAA 
GCTTATAACC CAGCCAAGTG CAGCGCCGAG AGTGAGGAGG ACAGGAAGGT CGTCTCGTTA 
CAGCTGGACA GGGATCACCA TGCTTTATAC GTGGCCTTCT CCAGCTGCGT GGTCCGCATC 
CCCCTCAGCC GCTGTGAGCG CTATGGCTCC TGTAAAAAGT CTTGCATTGC ATCACGAGAC 
CCGTACTGTG GTTGGTTAAG CCAGGGAGTG TGTGAGAGAG TGACCTTAGG GATGCTTGCT 
GGAGGATATG AGCAGGACAC CGAGTATGGC AACACGGCCC ACCTAGGGGA CTGCCACGGT 
GTACGGTGGG AAGTCCAGTC TGGAGATTCC AACCAGATGG TCCACATGAA TGTCCTCATC 
ACCTGCGTGT TTGCAGCTTT TGTCTTGGGC GCGTTCATCG CAGGAGTGGC TGTGTACTGC 
TATCGTGACA TGTTTGTTCG GAAGAACAGA AAGATCCATA AAGATGCAGA ATCGGCCCAG 
TCATGCACAG ATTCCAGTGG AAGCTTTGCC AAGCTGAATG GTCTCTTTGA CAGCCCCGTC 
AAGGAGTACC AGCAGAACAT CGATTCACCC AAACTGTACA GCAACCTGCT GACCAGTCGG 
AAGGAGCTGC CTCCCAACAC GGATCCAAAG TCCATGGCCA TGGACCATCG AGGCCAGCCT 
CCAGAGCTGG CTGCTCTCCC CACGCCAGAG TCTACACCTG TACTCCACCA GAAGACCCTG 
CAGGCCATGA AGAGCCACTC CGATAAGGCC CATGGCCATG GTGCTTCAAG GAAGGAACAC 
CCCCAGTTTT TTCCTTCTAG TCCTCCACCC CATTCCCCGT TAAGTCACGG GCATATTCCC 
AGTGCCATCG TTCTTCCAAA CGCCACTCAT GACTACAACA CATCCTTCTC AAACTCTAAC 
GCTCACAAAG CCGAAAAGAA GCTTCAGAAC GTTGATCACC CTCTCACAAA GTCATCCAGT 
AAGAGGGAAC ACCGGCGCTC TGTGGACTCC AGAAACACCC TCAATGATCT CTTGAAGCAT 
CTCAATGACC CAAACAGTAA CGCCAAAGCC ATCATGGGAG AAATCCACAT GGCCCATCAG 
ACCCTCATGC TGGACCCAGT GGGACCAATG TCTGAGGTCC CACCCAAGGT TCCTAACCGG 
GAGGCATCCC TATACTCCCC CCCCTCAACA CTCCCCAGAA ATAGTCCAAC CAAGAGAGTA 
GATGTCCCCA CCACTCCTGG GGTCCCAATG ACTTCTCTGG AAAGACAAAG GGGTTATCAC 
AAAAACTCCT CCCAGAGGCA CTCTATATCT GCCGTGCCTA AAAACTTAAA CTCACCAAAC 
GGTGTTTTGT TATCTAGACA GCCGAGTATG AACCGTGGAG GATATATGCC CACCCCAACA 
GGGGCGAAGG TGGACTATAT TCAGGGGACA CCGGTGAGTG TCCATCTGCA GCCCTCCCTC 
TCCAGACAGA GCAGCTACAC CAGTAATGGT ACCCTACCCA GGACGGGACT AAAGAGGACA 
CCATCCTTAA AACCTGATGT GCCACCAAAG CCTTCCTTTG TTCCTCAAAC CACATCTGTC 
AGACCACTGA ACAAATACAC TTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Stromal expression of decorin, Semaphorin6D, SPARC, Sprouty1 and Tsukushi in developing prostate and decreased levels of decorin in prostate cancer.
PloS one7(8)e42516(2012)
Henke A,Grace OC,Ashley GR,Stewart GD,Riddick AC,Yeun H,O'Donnell M,Anderson RA,Thomson AA

PlexinA1 signaling directs the segregation of proprioceptive sensory axons in the developing spinal cord.
Neuron52(5)775-88(2006 Dec)
Yoshida Y,Han B,Mendelsohn M,Jessell TM

Dual roles of Sema6D in cardiac morphogenesis through region-specific association of its receptor, Plexin-A1, with off-track and vascular endothelial growth factor receptor type 2.
Genes & development18(4)435-47(2004 Feb)
Toyofuku T,Zhang H,Kumanogoh A,Takegahara N,Suto F,Kamei J,Aoki K,Yabuki M,Hori M,Fujisawa H,Kikutani H

Identification, characterization, and functional study of the two novel human members of the semaphorin gene family.
The Journal of biological chemistry277(38)35574-85(2002 Sep)
Qu X,Wei H,Zhai Y,Que H,Chen Q,Tang F,Wu Y,Xing G,Zhu Y,Liu S,Fan M,He F

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Sema6d cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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