SEMA6DL cDNA ORF clone, Danio rerio(Zebrafish)

The following SEMA6DL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEMA6DL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa23486 XM_005173650.4
Latest version!
Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D, like (sema6dl), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa32512 NM_001017682.2
Latest version!
Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D, like (sema6dl), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa23484 XM_005173648.4
Latest version!
Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D, like (sema6dl), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa23484 XM_009293805.3
Latest version!
Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D, like (sema6dl), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa23485 XM_005173649.4
Latest version!
Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D, like (sema6dl), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa41756 XM_009293807.3
Latest version!
Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D, like (sema6dl), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
ODa41756 XM_009293807.2 Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D, like (sema6dl), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa23486 XM_005173650.3 Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D, like (sema6dl), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa23484 XM_009293805.2 Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D, like (sema6dl), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa23485 XM_005173649.3 Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D, like (sema6dl), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa23484 XM_005173648.3 Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D, like (sema6dl), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa23486
    Clone ID Related Accession (Same CDS sequence) XM_005173650.4 , XM_005173650.3
    Accession Version XM_005173650.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3000bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product semaphorin-6D isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173650.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGGTGGC GAGAACTGAC GATTGACCTG CTGATACTCC TGCTGGTGGC GTCCCGTCTG 
    GATGCTGTGA GCTTTCCTGA GGACAACATC CCTTTAGACG TTGTGGACAG ACACTATGCA
    CGGCAGTACC CGGTGTTTCG TGGACGTCCC TCTGGTAACG AATCTCAGCA CAGACTGGAC
    TTTCAGCTGA TGACCCAGAT ACATGATACG CTGTTCATCG CGGGCAGGGA TCAGGTATAT
    TTGGTGAGTC TGAGGGAGTC CTACAGGAAC GAGATCATTC CATACAGGAA GCTGACGTGG
    CGGTCGGGGC AGGCTGACAG AGAGACGTGC GCCATGAAGG GAAAACACAG AGACGAGTGC
    CATAATTTCA TTAAAGTCCT GGTTCCGAGA AATGATGATC TCGTGTTCAT CTGCGGAACA
    AATGGTTTCA ATCCCATGTG CCGTTACTAC AGGTTGGATA ACCTGGAATT TGATGGGGAA
    GAGATCAGCG GTTTGGCCCG ATGCCCGTTT GACGCTAAGC AGACAAACGT TGCCCTGTTT
    GCCGATGGTA AGCTGTACTC TGCTACGGTG GCAGACTTCC TGGCTAGCGA TGCTGTGATT
    TATCGCAGTA TGGGAGATGG TTCTGCCCTT CGCACTATCA AGTATGACTC CAAGTGGTTG
    AAAGAGCCAC ATTTTCTTCA CGCTGTGGAC TACGGGGATT TTGTCTACTT CTTCTTTAGA
    GAAATTGCAG CAGAGCACAA CAACTTGGGA AAGGCGGTTT ACTCCAGAGT GGCTCGGATC
    TGTAAAAATG ACATGGGAGG GTCTCAGCGG GTGCTGGAAA AACACTGGAC ATCTTTTGTT
    AAGGCTCGGC TGAACTGTTC CGTTCCTGGA GAATCGTTTT TCTACTTTGA CGTCCTTCAG
    GCCATTACCG ATATCATCGA CATTAATGGG GTGCCTTCTG TGGTTGGAGT TTTCACCACT
    CAGTTAAACA GTATTCCTGG ATCTGCAGTG TGTGCCTTCT CCATGGTGGA CATTGAGAAA
    GCCTTCCGCG GCCGTTTCAA AGAGCAGAAG ACGCCAGATT CAGTGTGGAC GCCATTTCCA
    GAGGAGAAAC TGCCCAAGCC CAGACCTGGC TGCTGTGCAG GACACGGTTC AGCAGAGTCC
    TACAAAACCT CCATCGAGTT TCCAGACGAG ACCCTTCAAT TCATCAAGTC ACACCCACTC
    ATGGACGCCT CGGTCCCCTC CATTGGAGAC GAACCGTGGT TCACCAAAAC AAGAGTCAGG
    TACAGACTAA CAGCACTTGC AGTTGACAAC ACGGCAGGAC CCCATAAGAA TTACACAGTG
    GTTTTCATCG GCTCAGAAGC AGGCATCGTG CTAAAAGTTC TAGCCAAGAC ATCCCCGCTG
    TCTTTGAATG ACAGCGTCCT GCTGGAGGAA ATTGACGTTT TCAACCAGGC CAAGTGTTTA
    TCCAGCAATG AGGACGATCG GCGAATTTTG TCTTTCCACC TAGATAAAGA CAGTCACACA
    CTTTATGTGG CCTTTTCCAG CTGTGTGGTA AGAATACCAC TCAGCCGCTG CGAACGACAC
    AGTTCCTGTC AAAAGTCTTG TATTGCTTCA AGAGACCCAT ACTGCGGATG GATGTCACAT
    GGAGCTTGTG AACGTATTCC TGCAACTATG CAAAGTGGTT ATGAGCAAGA TGTTGAATAT
    GGCAACACTG CTCACCTCGG GGATTGTCAC GGTGTTTGGG AAATACAAGC AGGTGAATCA
    AACCAGATGG TGCACATGAA CATCCTGATC TCCTGTGTGT TAGCTGCGTT TCTGCTTGGT
    GCATTCATCG CAGGCATGGT TGTTTACTGC TATAGAGATG CGTTTCTTCG CACTCCACGA
    AAGATCCAAA AAGATGCAGA GTCGGCACAA TCCTGCACTG ATTCCACTGG CAGCTTTGCC
    AAGCTTAACG GTCTGTTTGA CAGTCCTGTC AAAGAGTACC AGCCTAATTT GGATACTCCC
    AAACTCTACA CCAACCTGTT GAGCAATGGA AGGGAGGTGC CAACCACTGG AGAAACTAAG
    ACTATGCTCC TGAGTGGGCA GCCACCGGAA TTAGCAGCAC TGCCTACGCC TGAATCCACA
    CCTGTGCTGC AACAGAAGAG CCTCCAGCCC ATTAAGAACC AGTGGGAGAG AGCTCATGGA
    AAGATGAGTG GCTCCCGCAA AGATGCACCA CCCAAAAGCC CACAGTACCT TCCCTCTAGT
    CCACCTCCCC ATTCTGCAAT CAATCCACAC ATACCTAGTG CTGTGGTACT GCCTAATGCC
    ACGCATGAAA ACAAAGCAGG CTTCAGCACA CCAGAGGGGC CTCAAGCAGA AAAGAAGGTC
    CAGAATAGTG ATCAGCATGG TTCCAAATCC GGACGTAAAG ACCAGAGGCG AACAGTGGAT
    GCCAGGAATA CGTTAAACGA CCTCTTGAAA CATCTAAATG ATAGCAACCC CAAGGCCATC
    ATGGTAGAGA TGCCCAAATC TCGACAGCAC CTAATGCTGG AACCTATTGT AAGTCCAACA
    GAGATTCCAC CTAAAGTCCC AAGTCGAGAG GCCTCCTTGT ATTCTCCGTC TTCCTCGTTA
    CCAAGGAACA GCCCGACCAA GAGAGTGGAT GTGCCGACAA CGCCAACGTC GCCCACAGGG
    CATATGGGAA CTCTTGAGAG ACAGCGGTAT CACCGAGGCT CTTCCCAGCG ACACTCCATA
    TCCTCGCCGC CCAAAGGGCT GCACTCACCT AGCGGGGCCA TAGTGTCCCG GCAACCTAGC
    ATGAACAGAG GTGGGTACAT ACCCCCCACT CCTACCCCAC CCACTAGACT AGACTCTCAT
    GGGGTACCAA TGGCAATGAC GCCTTCCGTA TCCCGACAGA GCAGCTACAG TGGGCATGGA
    TCTTTACCAC GAACATCCAT TAAAAGGACA GCATCGCTAA AGCCCGATGT GCCACCCAAA
    CCCAACGGCT TTGCACCACA GACTGCACAA ATGAGGCCAG TGAACAAGTA TAGCTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173707.1
    CDS219..3218
    Translation

    Target ORF information:

    RefSeq Version XM_005173650.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D, like (sema6dl), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173650.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGGTGGC GAGAACTGAC GATTGACCTG CTGATACTCC TGCTGGTGGC GTCCCGTCTG 
    GATGCTGTGA GCTTTCCTGA GGACAACATC CCTTTAGACG TTGTGGACAG ACACTATGCA
    CGGCAGTACC CGGTGTTTCG TGGACGTCCC TCTGGTAACG AATCTCAGCA CAGACTGGAC
    TTTCAGCTGA TGACCCAGAT ACATGATACG CTGTTCATCG CGGGCAGGGA TCAGGTATAT
    TTGGTGAGTC TGAGGGAGTC CTACAGGAAC GAGATCATTC CATACAGGAA GCTGACGTGG
    CGGTCGGGGC AGGCTGACAG AGAGACGTGC GCCATGAAGG GAAAACACAG AGACGAGTGC
    CATAATTTCA TTAAAGTCCT GGTTCCGAGA AATGATGATC TCGTGTTCAT CTGCGGAACA
    AATGGTTTCA ATCCCATGTG CCGTTACTAC AGGTTGGATA ACCTGGAATT TGATGGGGAA
    GAGATCAGCG GTTTGGCCCG ATGCCCGTTT GACGCTAAGC AGACAAACGT TGCCCTGTTT
    GCCGATGGTA AGCTGTACTC TGCTACGGTG GCAGACTTCC TGGCTAGCGA TGCTGTGATT
    TATCGCAGTA TGGGAGATGG TTCTGCCCTT CGCACTATCA AGTATGACTC CAAGTGGTTG
    AAAGAGCCAC ATTTTCTTCA CGCTGTGGAC TACGGGGATT TTGTCTACTT CTTCTTTAGA
    GAAATTGCAG CAGAGCACAA CAACTTGGGA AAGGCGGTTT ACTCCAGAGT GGCTCGGATC
    TGTAAAAATG ACATGGGAGG GTCTCAGCGG GTGCTGGAAA AACACTGGAC ATCTTTTGTT
    AAGGCTCGGC TGAACTGTTC CGTTCCTGGA GAATCGTTTT TCTACTTTGA CGTCCTTCAG
    GCCATTACCG ATATCATCGA CATTAATGGG GTGCCTTCTG TGGTTGGAGT TTTCACCACT
    CAGTTAAACA GTATTCCTGG ATCTGCAGTG TGTGCCTTCT CCATGGTGGA CATTGAGAAA
    GCCTTCCGCG GCCGTTTCAA AGAGCAGAAG ACGCCAGATT CAGTGTGGAC GCCATTTCCA
    GAGGAGAAAC TGCCCAAGCC CAGACCTGGC TGCTGTGCAG GACACGGTTC AGCAGAGTCC
    TACAAAACCT CCATCGAGTT TCCAGACGAG ACCCTTCAAT TCATCAAGTC ACACCCACTC
    ATGGACGCCT CGGTCCCCTC CATTGGAGAC GAACCGTGGT TCACCAAAAC AAGAGTCAGG
    TACAGACTAA CAGCACTTGC AGTTGACAAC ACGGCAGGAC CCCATAAGAA TTACACAGTG
    GTTTTCATCG GCTCAGAAGC AGGCATCGTG CTAAAAGTTC TAGCCAAGAC ATCCCCGCTG
    TCTTTGAATG ACAGCGTCCT GCTGGAGGAA ATTGACGTTT TCAACCAGGC CAAGTGTTTA
    TCCAGCAATG AGGACGATCG GCGAATTTTG TCTTTCCACC TAGATAAAGA CAGTCACACA
    CTTTATGTGG CCTTTTCCAG CTGTGTGGTA AGAATACCAC TCAGCCGCTG CGAACGACAC
    AGTTCCTGTC AAAAGTCTTG TATTGCTTCA AGAGACCCAT ACTGCGGATG GATGTCACAT
    GGAGCTTGTG AACGTATTCC TGCAACTATG CAAAGTGGTT ATGAGCAAGA TGTTGAATAT
    GGCAACACTG CTCACCTCGG GGATTGTCAC GGTGTTTGGG AAATACAAGC AGGTGAATCA
    AACCAGATGG TGCACATGAA CATCCTGATC TCCTGTGTGT TAGCTGCGTT TCTGCTTGGT
    GCATTCATCG CAGGCATGGT TGTTTACTGC TATAGAGATG CGTTTCTTCG CACTCCACGA
    AAGATCCAAA AAGATGCAGA GTCGGCACAA TCCTGCACTG ATTCCACTGG CAGCTTTGCC
    AAGCTTAACG GTCTGTTTGA CAGTCCTGTC AAAGAGTACC AGCCTAATTT GGATACTCCC
    AAACTCTACA CCAACCTGTT GAGCAATGGA AGGGAGGTGC CAACCACTGG AGAAACTAAG
    ACTATGCTCC TGAGTGGGCA GCCACCGGAA TTAGCAGCAC TGCCTACGCC TGAATCCACA
    CCTGTGCTGC AACAGAAGAG CCTCCAGCCC ATTAAGAACC AGTGGGAGAG AGCTCATGGA
    AAGATGAGTG GCTCCCGCAA AGATGCACCA CCCAAAAGCC CACAGTACCT TCCCTCTAGT
    CCACCTCCCC ATTCTGCAAT CAATCCACAC ATACCTAGTG CTGTGGTACT GCCTAATGCC
    ACGCATGAAA ACAAAGCAGG CTTCAGCACA CCAGAGGGGC CTCAAGCAGA AAAGAAGGTC
    CAGAATAGTG ATCAGCATGG TTCCAAATCC GGACGTAAAG ACCAGAGGCG AACAGTGGAT
    GCCAGGAATA CGTTAAACGA CCTCTTGAAA CATCTAAATG ATAGCAACCC CAAGGCCATC
    ATGGTAGAGA TGCCCAAATC TCGACAGCAC CTAATGCTGG AACCTATTGT AAGTCCAACA
    GAGATTCCAC CTAAAGTCCC AAGTCGAGAG GCCTCCTTGT ATTCTCCGTC TTCCTCGTTA
    CCAAGGAACA GCCCGACCAA GAGAGTGGAT GTGCCGACAA CGCCAACGTC GCCCACAGGG
    CATATGGGAA CTCTTGAGAG ACAGCGGTAT CACCGAGGCT CTTCCCAGCG ACACTCCATA
    TCCTCGCCGC CCAAAGGGCT GCACTCACCT AGCGGGGCCA TAGTGTCCCG GCAACCTAGC
    ATGAACAGAG GTGGGTACAT ACCCCCCACT CCTACCCCAC CCACTAGACT AGACTCTCAT
    GGGGTACCAA TGGCAATGAC GCCTTCCGTA TCCCGACAGA GCAGCTACAG TGGGCATGGA
    TCTTTACCAC GAACATCCAT TAAAAGGACA GCATCGCTAA AGCCCGATGT GCCACCCAAA
    CCCAACGGCT TTGCACCACA GACTGCACAA ATGAGGCCAG TGAACAAGTA TAGCTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23486
    Clone ID Related Accession (Same CDS sequence) XM_005173650.4 , XM_005173650.3
    Accession Version XM_005173650.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3000bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product semaphorin-6D isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173650.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGGTGGC GAGAACTGAC GATTGACCTG CTGATACTCC TGCTGGTGGC GTCCCGTCTG 
    GATGCTGTGA GCTTTCCTGA GGACAACATC CCTTTAGACG TTGTGGACAG ACACTATGCA
    CGGCAGTACC CGGTGTTTCG TGGACGTCCC TCTGGTAACG AATCTCAGCA CAGACTGGAC
    TTTCAGCTGA TGACCCAGAT ACATGATACG CTGTTCATCG CGGGCAGGGA TCAGGTATAT
    TTGGTGAGTC TGAGGGAGTC CTACAGGAAC GAGATCATTC CATACAGGAA GCTGACGTGG
    CGGTCGGGGC AGGCTGACAG AGAGACGTGC GCCATGAAGG GAAAACACAG AGACGAGTGC
    CATAATTTCA TTAAAGTCCT GGTTCCGAGA AATGATGATC TCGTGTTCAT CTGCGGAACA
    AATGGTTTCA ATCCCATGTG CCGTTACTAC AGGTTGGATA ACCTGGAATT TGATGGGGAA
    GAGATCAGCG GTTTGGCCCG ATGCCCGTTT GACGCTAAGC AGACAAACGT TGCCCTGTTT
    GCCGATGGTA AGCTGTACTC TGCTACGGTG GCAGACTTCC TGGCTAGCGA TGCTGTGATT
    TATCGCAGTA TGGGAGATGG TTCTGCCCTT CGCACTATCA AGTATGACTC CAAGTGGTTG
    AAAGAGCCAC ATTTTCTTCA CGCTGTGGAC TACGGGGATT TTGTCTACTT CTTCTTTAGA
    GAAATTGCAG CAGAGCACAA CAACTTGGGA AAGGCGGTTT ACTCCAGAGT GGCTCGGATC
    TGTAAAAATG ACATGGGAGG GTCTCAGCGG GTGCTGGAAA AACACTGGAC ATCTTTTGTT
    AAGGCTCGGC TGAACTGTTC CGTTCCTGGA GAATCGTTTT TCTACTTTGA CGTCCTTCAG
    GCCATTACCG ATATCATCGA CATTAATGGG GTGCCTTCTG TGGTTGGAGT TTTCACCACT
    CAGTTAAACA GTATTCCTGG ATCTGCAGTG TGTGCCTTCT CCATGGTGGA CATTGAGAAA
    GCCTTCCGCG GCCGTTTCAA AGAGCAGAAG ACGCCAGATT CAGTGTGGAC GCCATTTCCA
    GAGGAGAAAC TGCCCAAGCC CAGACCTGGC TGCTGTGCAG GACACGGTTC AGCAGAGTCC
    TACAAAACCT CCATCGAGTT TCCAGACGAG ACCCTTCAAT TCATCAAGTC ACACCCACTC
    ATGGACGCCT CGGTCCCCTC CATTGGAGAC GAACCGTGGT TCACCAAAAC AAGAGTCAGG
    TACAGACTAA CAGCACTTGC AGTTGACAAC ACGGCAGGAC CCCATAAGAA TTACACAGTG
    GTTTTCATCG GCTCAGAAGC AGGCATCGTG CTAAAAGTTC TAGCCAAGAC ATCCCCGCTG
    TCTTTGAATG ACAGCGTCCT GCTGGAGGAA ATTGACGTTT TCAACCAGGC CAAGTGTTTA
    TCCAGCAATG AGGACGATCG GCGAATTTTG TCTTTCCACC TAGATAAAGA CAGTCACACA
    CTTTATGTGG CCTTTTCCAG CTGTGTGGTA AGAATACCAC TCAGCCGCTG CGAACGACAC
    AGTTCCTGTC AAAAGTCTTG TATTGCTTCA AGAGACCCAT ACTGCGGATG GATGTCACAT
    GGAGCTTGTG AACGTATTCC TGCAACTATG CAAAGTGGTT ATGAGCAAGA TGTTGAATAT
    GGCAACACTG CTCACCTCGG GGATTGTCAC GGTGTTTGGG AAATACAAGC AGGTGAATCA
    AACCAGATGG TGCACATGAA CATCCTGATC TCCTGTGTGT TAGCTGCGTT TCTGCTTGGT
    GCATTCATCG CAGGCATGGT TGTTTACTGC TATAGAGATG CGTTTCTTCG CACTCCACGA
    AAGATCCAAA AAGATGCAGA GTCGGCACAA TCCTGCACTG ATTCCACTGG CAGCTTTGCC
    AAGCTTAACG GTCTGTTTGA CAGTCCTGTC AAAGAGTACC AGCCTAATTT GGATACTCCC
    AAACTCTACA CCAACCTGTT GAGCAATGGA AGGGAGGTGC CAACCACTGG AGAAACTAAG
    ACTATGCTCC TGAGTGGGCA GCCACCGGAA TTAGCAGCAC TGCCTACGCC TGAATCCACA
    CCTGTGCTGC AACAGAAGAG CCTCCAGCCC ATTAAGAACC AGTGGGAGAG AGCTCATGGA
    AAGATGAGTG GCTCCCGCAA AGATGCACCA CCCAAAAGCC CACAGTACCT TCCCTCTAGT
    CCACCTCCCC ATTCTGCAAT CAATCCACAC ATACCTAGTG CTGTGGTACT GCCTAATGCC
    ACGCATGAAA ACAAAGCAGG CTTCAGCACA CCAGAGGGGC CTCAAGCAGA AAAGAAGGTC
    CAGAATAGTG ATCAGCATGG TTCCAAATCC GGACGTAAAG ACCAGAGGCG AACAGTGGAT
    GCCAGGAATA CGTTAAACGA CCTCTTGAAA CATCTAAATG ATAGCAACCC CAAGGCCATC
    ATGGTAGAGA TGCCCAAATC TCGACAGCAC CTAATGCTGG AACCTATTGT AAGTCCAACA
    GAGATTCCAC CTAAAGTCCC AAGTCGAGAG GCCTCCTTGT ATTCTCCGTC TTCCTCGTTA
    CCAAGGAACA GCCCGACCAA GAGAGTGGAT GTGCCGACAA CGCCAACGTC GCCCACAGGG
    CATATGGGAA CTCTTGAGAG ACAGCGGTAT CACCGAGGCT CTTCCCAGCG ACACTCCATA
    TCCTCGCCGC CCAAAGGGCT GCACTCACCT AGCGGGGCCA TAGTGTCCCG GCAACCTAGC
    ATGAACAGAG GTGGGTACAT ACCCCCCACT CCTACCCCAC CCACTAGACT AGACTCTCAT
    GGGGTACCAA TGGCAATGAC GCCTTCCGTA TCCCGACAGA GCAGCTACAG TGGGCATGGA
    TCTTTACCAC GAACATCCAT TAAAAGGACA GCATCGCTAA AGCCCGATGT GCCACCCAAA
    CCCAACGGCT TTGCACCACA GACTGCACAA ATGAGGCCAG TGAACAAGTA TAGCTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173707.1
    CDS443..3442
    Translation

    Target ORF information:

    RefSeq Version XM_005173650.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D, like (sema6dl), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173650.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGGTGGC GAGAACTGAC GATTGACCTG CTGATACTCC TGCTGGTGGC GTCCCGTCTG 
    GATGCTGTGA GCTTTCCTGA GGACAACATC CCTTTAGACG TTGTGGACAG ACACTATGCA
    CGGCAGTACC CGGTGTTTCG TGGACGTCCC TCTGGTAACG AATCTCAGCA CAGACTGGAC
    TTTCAGCTGA TGACCCAGAT ACATGATACG CTGTTCATCG CGGGCAGGGA TCAGGTATAT
    TTGGTGAGTC TGAGGGAGTC CTACAGGAAC GAGATCATTC CATACAGGAA GCTGACGTGG
    CGGTCGGGGC AGGCTGACAG AGAGACGTGC GCCATGAAGG GAAAACACAG AGACGAGTGC
    CATAATTTCA TTAAAGTCCT GGTTCCGAGA AATGATGATC TCGTGTTCAT CTGCGGAACA
    AATGGTTTCA ATCCCATGTG CCGTTACTAC AGGTTGGATA ACCTGGAATT TGATGGGGAA
    GAGATCAGCG GTTTGGCCCG ATGCCCGTTT GACGCTAAGC AGACAAACGT TGCCCTGTTT
    GCCGATGGTA AGCTGTACTC TGCTACGGTG GCAGACTTCC TGGCTAGCGA TGCTGTGATT
    TATCGCAGTA TGGGAGATGG TTCTGCCCTT CGCACTATCA AGTATGACTC CAAGTGGTTG
    AAAGAGCCAC ATTTTCTTCA CGCTGTGGAC TACGGGGATT TTGTCTACTT CTTCTTTAGA
    GAAATTGCAG CAGAGCACAA CAACTTGGGA AAGGCGGTTT ACTCCAGAGT GGCTCGGATC
    TGTAAAAATG ACATGGGAGG GTCTCAGCGG GTGCTGGAAA AACACTGGAC ATCTTTTGTT
    AAGGCTCGGC TGAACTGTTC CGTTCCTGGA GAATCGTTTT TCTACTTTGA CGTCCTTCAG
    GCCATTACCG ATATCATCGA CATTAATGGG GTGCCTTCTG TGGTTGGAGT TTTCACCACT
    CAGTTAAACA GTATTCCTGG ATCTGCAGTG TGTGCCTTCT CCATGGTGGA CATTGAGAAA
    GCCTTCCGCG GCCGTTTCAA AGAGCAGAAG ACGCCAGATT CAGTGTGGAC GCCATTTCCA
    GAGGAGAAAC TGCCCAAGCC CAGACCTGGC TGCTGTGCAG GACACGGTTC AGCAGAGTCC
    TACAAAACCT CCATCGAGTT TCCAGACGAG ACCCTTCAAT TCATCAAGTC ACACCCACTC
    ATGGACGCCT CGGTCCCCTC CATTGGAGAC GAACCGTGGT TCACCAAAAC AAGAGTCAGG
    TACAGACTAA CAGCACTTGC AGTTGACAAC ACGGCAGGAC CCCATAAGAA TTACACAGTG
    GTTTTCATCG GCTCAGAAGC AGGCATCGTG CTAAAAGTTC TAGCCAAGAC ATCCCCGCTG
    TCTTTGAATG ACAGCGTCCT GCTGGAGGAA ATTGACGTTT TCAACCAGGC CAAGTGTTTA
    TCCAGCAATG AGGACGATCG GCGAATTTTG TCTTTCCACC TAGATAAAGA CAGTCACACA
    CTTTATGTGG CCTTTTCCAG CTGTGTGGTA AGAATACCAC TCAGCCGCTG CGAACGACAC
    AGTTCCTGTC AAAAGTCTTG TATTGCTTCA AGAGACCCAT ACTGCGGATG GATGTCACAT
    GGAGCTTGTG AACGTATTCC TGCAACTATG CAAAGTGGTT ATGAGCAAGA TGTTGAATAT
    GGCAACACTG CTCACCTCGG GGATTGTCAC GGTGTTTGGG AAATACAAGC AGGTGAATCA
    AACCAGATGG TGCACATGAA CATCCTGATC TCCTGTGTGT TAGCTGCGTT TCTGCTTGGT
    GCATTCATCG CAGGCATGGT TGTTTACTGC TATAGAGATG CGTTTCTTCG CACTCCACGA
    AAGATCCAAA AAGATGCAGA GTCGGCACAA TCCTGCACTG ATTCCACTGG CAGCTTTGCC
    AAGCTTAACG GTCTGTTTGA CAGTCCTGTC AAAGAGTACC AGCCTAATTT GGATACTCCC
    AAACTCTACA CCAACCTGTT GAGCAATGGA AGGGAGGTGC CAACCACTGG AGAAACTAAG
    ACTATGCTCC TGAGTGGGCA GCCACCGGAA TTAGCAGCAC TGCCTACGCC TGAATCCACA
    CCTGTGCTGC AACAGAAGAG CCTCCAGCCC ATTAAGAACC AGTGGGAGAG AGCTCATGGA
    AAGATGAGTG GCTCCCGCAA AGATGCACCA CCCAAAAGCC CACAGTACCT TCCCTCTAGT
    CCACCTCCCC ATTCTGCAAT CAATCCACAC ATACCTAGTG CTGTGGTACT GCCTAATGCC
    ACGCATGAAA ACAAAGCAGG CTTCAGCACA CCAGAGGGGC CTCAAGCAGA AAAGAAGGTC
    CAGAATAGTG ATCAGCATGG TTCCAAATCC GGACGTAAAG ACCAGAGGCG AACAGTGGAT
    GCCAGGAATA CGTTAAACGA CCTCTTGAAA CATCTAAATG ATAGCAACCC CAAGGCCATC
    ATGGTAGAGA TGCCCAAATC TCGACAGCAC CTAATGCTGG AACCTATTGT AAGTCCAACA
    GAGATTCCAC CTAAAGTCCC AAGTCGAGAG GCCTCCTTGT ATTCTCCGTC TTCCTCGTTA
    CCAAGGAACA GCCCGACCAA GAGAGTGGAT GTGCCGACAA CGCCAACGTC GCCCACAGGG
    CATATGGGAA CTCTTGAGAG ACAGCGGTAT CACCGAGGCT CTTCCCAGCG ACACTCCATA
    TCCTCGCCGC CCAAAGGGCT GCACTCACCT AGCGGGGCCA TAGTGTCCCG GCAACCTAGC
    ATGAACAGAG GTGGGTACAT ACCCCCCACT CCTACCCCAC CCACTAGACT AGACTCTCAT
    GGGGTACCAA TGGCAATGAC GCCTTCCGTA TCCCGACAGA GCAGCTACAG TGGGCATGGA
    TCTTTACCAC GAACATCCAT TAAAAGGACA GCATCGCTAA AGCCCGATGT GCCACCCAAA
    CCCAACGGCT TTGCACCACA GACTGCACAA ATGAGGCCAG TGAACAAGTA TAGCTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa32512
    Clone ID Related Accession (Same CDS sequence) NM_001017682.2
    Accession Version NM_001017682.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3057bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-23
    Organism Danio rerio(zebrafish)
    Product semaphorin-6D precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB212073.1. On Oct 20, 2007 this sequence version replaced NM_001017682.1. ##Evidence-Data-START## Transcript exon combination :: AB212073.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGGGTGGC GAGAACTGAC GATTGACCTG CTGATACTCC TGCTGGTGGC GTCCCGTCTG 
    GATGCTGTGA GCTTTCCTGA GGACAACATC CCTTTAGACG TTGTGGACAG ACACTATGCA
    CGGCAGTACC CGGTGTTTCG TGGACGTCCC TCTGGTAACG AATCTCAGCA CAGACTGGAC
    TTTCAGCTGA TGACCCAGAT ACATGATACG CTGTTCATCG CGGGCAGGGA TCAGGTATAT
    TTGGTGAGTC TGAGGGAGTC CTACAGGAAC GAGATCATTC CATACAGGAA GCTGACGTGG
    CGGTCGGGGC AGGCTGACAG AGAGACGTGC GCCATGAAGG GAAAACACAG AGACGAGTGC
    CATAATTTCA TTAAAGTCCT GGTTCCGAGA AATGATGATC TCGTGTTCAT CTGCGGAACA
    AATGGTTTCA ATCCCATGTG CCGTTACTAC AGGTTGGATA ACCTGGAATT TGATGGGGAA
    GAGATCAGCG GTTTGGCCCG ATGCCCGTTT GACGCTAAGC AGACAAACGT TGCCCTGTTT
    GCCGATGGTA AGCTGTACTC TGCTACGGTG GCAGACTTCC TGGCTAGCGA TGCTGTGATT
    TATCGCAGTA TGGGAGATGG TTCTGCCCTT CGCACTATCA AGTATGACTC CAAGTGGTTG
    AAAGAGCCAC ATTTTCTTCA CGCTGTGGAC TACGGGGATT TTGTCTACTT CTTCTTTAGA
    GAAATTGCAG CAGAGCACAA CAACTTGGGA AAGGCGGTTT ACTCCAGAGT GGCTCGGATC
    TGTAAAAATG ACATGGGAGG GTCTCAGCGG GTGCTGGAAA AACACTGGAC ATCTTTTGTT
    AAGGCTCGGC TGAACTGTTC CGTTCCTGGA GAATCGTTTT TCTACTTTGA CGTCCTTCAG
    GCCATTACCG ATATCATCGA CATTAATGGG GTGCCTTCTG TGGTTGGAGT TTTCACCACT
    CAGTTAAACA GTATTCCTGG ATCTGCAGTG TGTGCCTTCT CCATGGTGGA CATTGAGAAA
    GCCTTCCGCG GCCGTTTCAA AGAGCAGAAG ACGCCAGATT CAGTGTGGAC GCCATTTCCA
    GAGGAGAAAC TGCCCAAGCC CAGACCTGGC TGCTGTGCAG GACACGGTTC AGCAGAGTCC
    TACAAAACCT CCATCGAGTT TCCAGACGAG ACCCTTCAAT TCATCAAGTC ACACCCACTC
    ATGGACGCCT CGGTCCCCTC CATTGGAGAC GAACCGTGGT TCACCAAAAC AAGAGTCAGG
    TACAGACTAA CAGCACTTGC AGTTGACAAC ACGGCAGGAC CCCATAAGAA TTACACAGTG
    GTTTTCATCG GCTCAGAAGC AGGCATCGTG CTAAAAGTTC TAGCCAAGAC ATCCCCGCTG
    TCTTTGAATG ACAGCGTCCT GCTGGAGGAA ATTGACGTTT TCAACCAGGC CAAGTGTTTA
    TCCAGCAATG AGGACGATCG GCGAATTTTG TCTTTCCACC TAGATAAAGA CAGTCACACA
    CTTTATGTGG CCTTTTCCAG CTGTGTGGTA AGAATACCAC TCAGCCGCTG CGAACGACAC
    AGTTCCTGTC AAAAGTCTTG TATTGCTTCA AGAGACCCAT ACTGCGGATG GATGTCACAT
    GGAGCTTGTG AACGTATTCC TGCAACTATG CAAAGTGGTT ATGAGCAAGA TGTTGAATAT
    GGCAACACTG CTCACCTCGG GGATTGTCAC GAATTTTTGG CCACTACATC AGCGCCAGAT
    TTCAAATCAT ATGGCGACCC AACCTCTGGT GTTTGGGAAA TACAAGCAGG TGAATCAAAC
    CAGATGGTGC ACATGAACAT CCTGATCTCC TGTGTGTTAG CTGCGTTTCT GCTTGGTGCA
    TTCATCGCAG GCATGGTTGT TTACTGCTAT AGAGATGCGT TTCTTCGCAC TCCACGAAAG
    ATCCAAAAAG ATGCAGAGTC GGCACAATCC TGCACTGATT CCACTGGCAG CTTTGCCAAG
    CTTAACGGTC TGTTTGACAG TCCTGTCAAA GAGTACCAGC CTAATTTGGA TACTCCCAAA
    CTCTACACCA ACCTGTTGAG CAATGGAAGG GAGGTGCCAA CCACTGGAGA AACTAAGACT
    ATGCTCCTGA GTGGGCAGCC ACCGGAATTA GCAGCACTGC CTACGCCTGA ATCCACACCT
    GTGCTGCAAC AGAAGAGCCT CCAGCCCATT AAGAACCAGT GGGAGAGAGC TCATGGAAAG
    ATGAGTGGCT CCCGCAAAGA TGCACCACCC AAAAGCCCAC AGTACCTTCC CTCTAGTCCA
    CCTCCCCATT CTGCAATCAA TCCACACATA CCTAGTGCTG TGGTACTGCC TAATGCCACG
    CATGAAAACA AAGCAGGCTT CAGCACACCA GAGGGGCCTC AAGCAGAAAA GAAGGTCCAG
    AATAGTGATC AGCATGGTTC CAAATCCGGA CGTAAAGACC AGAGGCGAAC AGTGGATGCC
    AGGAATACGT TAAACGACCT CTTGAAACAT CTAAATGATA GCAACCCCAA GGCCATCATG
    GTAGAGATGC CCAAATCTCG ACAGCACCTA ATGCTGGAAC CTATTGTAAG TCCAACAGAG
    ATTCCACCTA AAGTCCCAAG TCGAGAGGCC TCCTTGTATT CTCCGTCTTC CTCGTTACCA
    AGGAACAGCC CGACCAAGAG AGTGGATGTG CCGACAACGC CAACGTCGCC CACAGGGCAT
    ATGGGAACTC TTGAGAGACA GCGGTATCAC CGAGGCTCTT CCCAGCGACA CTCCATATCC
    TCGCCGCCCA AAGGGCTGCA CTCACCTAGC GGGGCCATAG TGTCCCGGCA ACCTAGCATG
    AACAGAGGTG GGTACATACC CCCCACTCCT ACCCCACCCA CTAGACTAGA CTCTCATGGG
    GTACCAATGG CAATGACGCC TTCCGTATCC CGACAGAGCA GCTACAGTGG GCATGGATCT
    TTACCACGAA CATCCATTAA AAGGACAGCA TCGCTAAAGC CCGATGTGCC ACCCAAACCC
    AACGGCTTTG CACCACAGAC TGCACAAATG AGGCCAGTGA ACAAGTATAG CTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001017682.2
    CDS101..3157
    Translation

    Target ORF information:

    RefSeq Version NM_001017682.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D, like (sema6dl), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001017682.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGGGTGGC GAGAACTGAC GATTGACCTG CTGATACTCC TGCTGGTGGC GTCCCGTCTG 
    GATGCTGTGA GCTTTCCTGA GGACAACATC CCTTTAGACG TTGTGGACAG ACACTATGCA
    CGGCAGTACC CGGTGTTTCG TGGACGTCCC TCTGGTAACG AATCTCAGCA CAGACTGGAC
    TTTCAGCTGA TGACCCAGAT ACATGATACG CTGTTCATCG CGGGCAGGGA TCAGGTATAT
    TTGGTGAGTC TGAGGGAGTC CTACAGGAAC GAGATCATTC CATACAGGAA GCTGACGTGG
    CGGTCGGGGC AGGCTGACAG AGAGACGTGC GCCATGAAGG GAAAACACAG AGACGAGTGC
    CATAATTTCA TTAAAGTCCT GGTTCCGAGA AATGATGATC TCGTGTTCAT CTGCGGAACA
    AATGGTTTCA ATCCCATGTG CCGTTACTAC AGGTTGGATA ACCTGGAATT TGATGGGGAA
    GAGATCAGCG GTTTGGCCCG ATGCCCGTTT GACGCTAAGC AGACAAACGT TGCCCTGTTT
    GCCGATGGTA AGCTGTACTC TGCTACGGTG GCAGACTTCC TGGCTAGCGA TGCTGTGATT
    TATCGCAGTA TGGGAGATGG TTCTGCCCTT CGCACTATCA AGTATGACTC CAAGTGGTTG
    AAAGAGCCAC ATTTTCTTCA CGCTGTGGAC TACGGGGATT TTGTCTACTT CTTCTTTAGA
    GAAATTGCAG CAGAGCACAA CAACTTGGGA AAGGCGGTTT ACTCCAGAGT GGCTCGGATC
    TGTAAAAATG ACATGGGAGG GTCTCAGCGG GTGCTGGAAA AACACTGGAC ATCTTTTGTT
    AAGGCTCGGC TGAACTGTTC CGTTCCTGGA GAATCGTTTT TCTACTTTGA CGTCCTTCAG
    GCCATTACCG ATATCATCGA CATTAATGGG GTGCCTTCTG TGGTTGGAGT TTTCACCACT
    CAGTTAAACA GTATTCCTGG ATCTGCAGTG TGTGCCTTCT CCATGGTGGA CATTGAGAAA
    GCCTTCCGCG GCCGTTTCAA AGAGCAGAAG ACGCCAGATT CAGTGTGGAC GCCATTTCCA
    GAGGAGAAAC TGCCCAAGCC CAGACCTGGC TGCTGTGCAG GACACGGTTC AGCAGAGTCC
    TACAAAACCT CCATCGAGTT TCCAGACGAG ACCCTTCAAT TCATCAAGTC ACACCCACTC
    ATGGACGCCT CGGTCCCCTC CATTGGAGAC GAACCGTGGT TCACCAAAAC AAGAGTCAGG
    TACAGACTAA CAGCACTTGC AGTTGACAAC ACGGCAGGAC CCCATAAGAA TTACACAGTG
    GTTTTCATCG GCTCAGAAGC AGGCATCGTG CTAAAAGTTC TAGCCAAGAC ATCCCCGCTG
    TCTTTGAATG ACAGCGTCCT GCTGGAGGAA ATTGACGTTT TCAACCAGGC CAAGTGTTTA
    TCCAGCAATG AGGACGATCG GCGAATTTTG TCTTTCCACC TAGATAAAGA CAGTCACACA
    CTTTATGTGG CCTTTTCCAG CTGTGTGGTA AGAATACCAC TCAGCCGCTG CGAACGACAC
    AGTTCCTGTC AAAAGTCTTG TATTGCTTCA AGAGACCCAT ACTGCGGATG GATGTCACAT
    GGAGCTTGTG AACGTATTCC TGCAACTATG CAAAGTGGTT ATGAGCAAGA TGTTGAATAT
    GGCAACACTG CTCACCTCGG GGATTGTCAC GAATTTTTGG CCACTACATC AGCGCCAGAT
    TTCAAATCAT ATGGCGACCC AACCTCTGGT GTTTGGGAAA TACAAGCAGG TGAATCAAAC
    CAGATGGTGC ACATGAACAT CCTGATCTCC TGTGTGTTAG CTGCGTTTCT GCTTGGTGCA
    TTCATCGCAG GCATGGTTGT TTACTGCTAT AGAGATGCGT TTCTTCGCAC TCCACGAAAG
    ATCCAAAAAG ATGCAGAGTC GGCACAATCC TGCACTGATT CCACTGGCAG CTTTGCCAAG
    CTTAACGGTC TGTTTGACAG TCCTGTCAAA GAGTACCAGC CTAATTTGGA TACTCCCAAA
    CTCTACACCA ACCTGTTGAG CAATGGAAGG GAGGTGCCAA CCACTGGAGA AACTAAGACT
    ATGCTCCTGA GTGGGCAGCC ACCGGAATTA GCAGCACTGC CTACGCCTGA ATCCACACCT
    GTGCTGCAAC AGAAGAGCCT CCAGCCCATT AAGAACCAGT GGGAGAGAGC TCATGGAAAG
    ATGAGTGGCT CCCGCAAAGA TGCACCACCC AAAAGCCCAC AGTACCTTCC CTCTAGTCCA
    CCTCCCCATT CTGCAATCAA TCCACACATA CCTAGTGCTG TGGTACTGCC TAATGCCACG
    CATGAAAACA AAGCAGGCTT CAGCACACCA GAGGGGCCTC AAGCAGAAAA GAAGGTCCAG
    AATAGTGATC AGCATGGTTC CAAATCCGGA CGTAAAGACC AGAGGCGAAC AGTGGATGCC
    AGGAATACGT TAAACGACCT CTTGAAACAT CTAAATGATA GCAACCCCAA GGCCATCATG
    GTAGAGATGC CCAAATCTCG ACAGCACCTA ATGCTGGAAC CTATTGTAAG TCCAACAGAG
    ATTCCACCTA AAGTCCCAAG TCGAGAGGCC TCCTTGTATT CTCCGTCTTC CTCGTTACCA
    AGGAACAGCC CGACCAAGAG AGTGGATGTG CCGACAACGC CAACGTCGCC CACAGGGCAT
    ATGGGAACTC TTGAGAGACA GCGGTATCAC CGAGGCTCTT CCCAGCGACA CTCCATATCC
    TCGCCGCCCA AAGGGCTGCA CTCACCTAGC GGGGCCATAG TGTCCCGGCA ACCTAGCATG
    AACAGAGGTG GGTACATACC CCCCACTCCT ACCCCACCCA CTAGACTAGA CTCTCATGGG
    GTACCAATGG CAATGACGCC TTCCGTATCC CGACAGAGCA GCTACAGTGG GCATGGATCT
    TTACCACGAA CATCCATTAA AAGGACAGCA TCGCTAAAGC CCGATGTGCC ACCCAAACCC
    AACGGCTTTG CACCACAGAC TGCACAAATG AGGCCAGTGA ACAAGTATAG CTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23484
    Clone ID Related Accession (Same CDS sequence) XM_005173648.4 , XM_009293805.2 , XM_009293805.3 , XM_005173648.3
    Accession Version XM_005173648.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3213bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product semaphorin-6D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173648.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTGGC GAGAACTGAC GATTGACCTG CTGATACTCC TGCTGGTGGC GTCCCGTCTG 
    GATGCTGTGA GCTTTCCTGA GGACAACATC CCTTTAGACG TTGTGGACAG ACACTATGCA
    CGGCAGTACC CGGTGTTTCG TGGACGTCCC TCTGGTAACG AATCTCAGCA CAGACTGGAC
    TTTCAGCTGA TGACCCAGAT ACATGATACG CTGTTCATCG CGGGCAGGGA TCAGGTATAT
    TTGGTGAGTC TGAGGGAGTC CTACAGGAAC GAGATCATTC CATACAGGAA GCTGACGTGG
    CGGTCGGGGC AGGCTGACAG AGAGACGTGC GCCATGAAGG GAAAACACAG AGACGAGTGC
    CATAATTTCA TTAAAGTCCT GGTTCCGAGA AATGATGATC TCGTGTTCAT CTGCGGAACA
    AATGGTTTCA ATCCCATGTG CCGTTACTAC AGGTTGGATA ACCTGGAATT TGATGGGGAA
    GAGATCAGCG GTTTGGCCCG ATGCCCGTTT GACGCTAAGC AGACAAACGT TGCCCTGTTT
    GCCGATGGTA AGCTGTACTC TGCTACGGTG GCAGACTTCC TGGCTAGCGA TGCTGTGATT
    TATCGCAGTA TGGGAGATGG TTCTGCCCTT CGCACTATCA AGTATGACTC CAAGTGGTTG
    AAAGAGCCAC ATTTTCTTCA CGCTGTGGAC TACGGGGATT TTGTCTACTT CTTCTTTAGA
    GAAATTGCAG CAGAGCACAA CAACTTGGGA AAGGCGGTTT ACTCCAGAGT GGCTCGGATC
    TGTAAAAATG ACATGGGAGG GTCTCAGCGG GTGCTGGAAA AACACTGGAC ATCTTTTGTT
    AAGGCTCGGC TGAACTGTTC CGTTCCTGGA GAATCGTTTT TCTACTTTGA CGTCCTTCAG
    GCCATTACCG ATATCATCGA CATTAATGGG GTGCCTTCTG TGGTTGGAGT TTTCACCACT
    CAGTTAAACA GTATTCCTGG ATCTGCAGTG TGTGCCTTCT CCATGGTGGA CATTGAGAAA
    GCCTTCCGCG GCCGTTTCAA AGAGCAGAAG ACGCCAGATT CAGTGTGGAC GCCATTTCCA
    GAGGAGAAAC TGCCCAAGCC CAGACCTGGC TGCTGTGCAG GACACGGTTC AGCAGAGTCC
    TACAAAACCT CCATCGAGTT TCCAGACGAG ACCCTTCAAT TCATCAAGTC ACACCCACTC
    ATGGACGCCT CGGTCCCCTC CATTGGAGAC GAACCGTGGT TCACCAAAAC AAGAGTCAGG
    TACAGACTAA CAGCACTTGC AGTTGACAAC ACGGCAGGAC CCCATAAGAA TTACACAGTG
    GTTTTCATCG GCTCAGAAGC AGGCATCGTG CTAAAAGTTC TAGCCAAGAC ATCCCCGCTG
    TCTTTGAATG ACAGCGTCCT GCTGGAGGAA ATTGACGTTT TCAACCAGGC CAAGTGTTTA
    TCCAGCAATG AGGACGATCG GCGAATTTTG TCTTTCCACC TAGATAAAGA CAGTCACACA
    CTTTATGTGG CCTTTTCCAG CTGTGTGGTA AGAATACCAC TCAGCCGCTG CGAACGACAC
    AGTTCCTGTC AAAAGTCTTG TATTGCTTCA AGAGACCCAT ACTGCGGATG GATGTCACAT
    GGAGCTTGTG AACGTATTCC TGCAACTATG CAAAGTGGTT ATGAGCAAGA TGTTGAATAT
    GGCAACACTG CTCACCTCGG GGATTGTCAC GAATTTTTGG CCACTACATC AGCGCCAGAT
    TTCAAATCAT ATGGCGACCC AACCTCTGAC ATGGAGTTGC CATCTTCATC AGTCACAGTG
    CCCGCGACTT CTGAACCCAT ACAATCACCC CAAACCAACC CCACTCAGAC ACCACAACTC
    TTTGAACCCC ATGGTGACCC AGCCACTTCA CATTCTGTTG AGCATATACC AGGTGTGCTG
    GAGGGTGTTT GGGAAATACA AGCAGGTGAA TCAAACCAGA TGGTGCACAT GAACATCCTG
    ATCTCCTGTG TGTTAGCTGC GTTTCTGCTT GGTGCATTCA TCGCAGGCAT GGTTGTTTAC
    TGCTATAGAG ATGCGTTTCT TCGCACTCCA CGAAAGATCC AAAAAGATGC AGAGTCGGCA
    CAATCCTGCA CTGATTCCAC TGGCAGCTTT GCCAAGCTTA ACGGTCTGTT TGACAGTCCT
    GTCAAAGAGT ACCAGCCTAA TTTGGATACT CCCAAACTCT ACACCAACCT GTTGAGCAAT
    GGAAGGGAGG TGCCAACCAC TGGAGAAACT AAGACTATGC TCCTGAGTGG GCAGCCACCG
    GAATTAGCAG CACTGCCTAC GCCTGAATCC ACACCTGTGC TGCAACAGAA GAGCCTCCAG
    CCCATTAAGA ACCAGTGGGA GAGAGCTCAT GGAAAGATGA GTGGCTCCCG CAAAGATGCA
    CCACCCAAAA GCCCACAGTA CCTTCCCTCT AGTCCACCTC CCCATTCTGC AATCAATCCA
    CACATACCTA GTGCTGTGGT ACTGCCTAAT GCCACGCATG AAAACAAAGC AGGCTTCAGC
    ACACCAGAGG GGCCTCAAGC AGAAAAGAAG GTCCAGAATA GTGATCAGCA TGGTTCCAAA
    TCCGGACGTA AAGACCAGAG GCGAACAGTG GATGCCAGGA ATACGTTAAA CGACCTCTTG
    AAACATCTAA ATGATAGCAA CCCCAAGGCC ATCATGGTAG AGATGCCCAA ATCTCGACAG
    CACCTAATGC TGGAACCTAT TGTAAGTCCA ACAGAGATTC CACCTAAAGT CCCAAGTCGA
    GAGGCCTCCT TGTATTCTCC GTCTTCCTCG TTACCAAGGA ACAGCCCGAC CAAGAGAGTG
    GATGTGCCGA CAACGCCAAC GTCGCCCACA GGGCATATGG GAACTCTTGA GAGACAGCGG
    TATCACCGAG GCTCTTCCCA GCGACACTCC ATATCCTCGC CGCCCAAAGG GCTGCACTCA
    CCTAGCGGGG CCATAGTGTC CCGGCAACCT AGCATGAACA GAGGTGGGTA CATACCCCCC
    ACTCCTACCC CACCCACTAG ACTAGACTCT CATGGGGTAC CAATGGCAAT GACGCCTTCC
    GTATCCCGAC AGAGCAGCTA CAGTGGGCAT GGATCTTTAC CACGAACATC CATTAAAAGG
    ACAGCATCGC TAAAGCCCGA TGTGCCACCC AAACCCAACG GCTTTGCACC ACAGACTGCA
    CAAATGAGGC CAGTGAACAA GTATAGCTAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173705.1
    CDS219..3431
    Translation

    Target ORF information:

    RefSeq Version XM_005173648.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D, like (sema6dl), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173648.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTGGC GAGAACTGAC GATTGACCTG CTGATACTCC TGCTGGTGGC GTCCCGTCTG 
    GATGCTGTGA GCTTTCCTGA GGACAACATC CCTTTAGACG TTGTGGACAG ACACTATGCA
    CGGCAGTACC CGGTGTTTCG TGGACGTCCC TCTGGTAACG AATCTCAGCA CAGACTGGAC
    TTTCAGCTGA TGACCCAGAT ACATGATACG CTGTTCATCG CGGGCAGGGA TCAGGTATAT
    TTGGTGAGTC TGAGGGAGTC CTACAGGAAC GAGATCATTC CATACAGGAA GCTGACGTGG
    CGGTCGGGGC AGGCTGACAG AGAGACGTGC GCCATGAAGG GAAAACACAG AGACGAGTGC
    CATAATTTCA TTAAAGTCCT GGTTCCGAGA AATGATGATC TCGTGTTCAT CTGCGGAACA
    AATGGTTTCA ATCCCATGTG CCGTTACTAC AGGTTGGATA ACCTGGAATT TGATGGGGAA
    GAGATCAGCG GTTTGGCCCG ATGCCCGTTT GACGCTAAGC AGACAAACGT TGCCCTGTTT
    GCCGATGGTA AGCTGTACTC TGCTACGGTG GCAGACTTCC TGGCTAGCGA TGCTGTGATT
    TATCGCAGTA TGGGAGATGG TTCTGCCCTT CGCACTATCA AGTATGACTC CAAGTGGTTG
    AAAGAGCCAC ATTTTCTTCA CGCTGTGGAC TACGGGGATT TTGTCTACTT CTTCTTTAGA
    GAAATTGCAG CAGAGCACAA CAACTTGGGA AAGGCGGTTT ACTCCAGAGT GGCTCGGATC
    TGTAAAAATG ACATGGGAGG GTCTCAGCGG GTGCTGGAAA AACACTGGAC ATCTTTTGTT
    AAGGCTCGGC TGAACTGTTC CGTTCCTGGA GAATCGTTTT TCTACTTTGA CGTCCTTCAG
    GCCATTACCG ATATCATCGA CATTAATGGG GTGCCTTCTG TGGTTGGAGT TTTCACCACT
    CAGTTAAACA GTATTCCTGG ATCTGCAGTG TGTGCCTTCT CCATGGTGGA CATTGAGAAA
    GCCTTCCGCG GCCGTTTCAA AGAGCAGAAG ACGCCAGATT CAGTGTGGAC GCCATTTCCA
    GAGGAGAAAC TGCCCAAGCC CAGACCTGGC TGCTGTGCAG GACACGGTTC AGCAGAGTCC
    TACAAAACCT CCATCGAGTT TCCAGACGAG ACCCTTCAAT TCATCAAGTC ACACCCACTC
    ATGGACGCCT CGGTCCCCTC CATTGGAGAC GAACCGTGGT TCACCAAAAC AAGAGTCAGG
    TACAGACTAA CAGCACTTGC AGTTGACAAC ACGGCAGGAC CCCATAAGAA TTACACAGTG
    GTTTTCATCG GCTCAGAAGC AGGCATCGTG CTAAAAGTTC TAGCCAAGAC ATCCCCGCTG
    TCTTTGAATG ACAGCGTCCT GCTGGAGGAA ATTGACGTTT TCAACCAGGC CAAGTGTTTA
    TCCAGCAATG AGGACGATCG GCGAATTTTG TCTTTCCACC TAGATAAAGA CAGTCACACA
    CTTTATGTGG CCTTTTCCAG CTGTGTGGTA AGAATACCAC TCAGCCGCTG CGAACGACAC
    AGTTCCTGTC AAAAGTCTTG TATTGCTTCA AGAGACCCAT ACTGCGGATG GATGTCACAT
    GGAGCTTGTG AACGTATTCC TGCAACTATG CAAAGTGGTT ATGAGCAAGA TGTTGAATAT
    GGCAACACTG CTCACCTCGG GGATTGTCAC GAATTTTTGG CCACTACATC AGCGCCAGAT
    TTCAAATCAT ATGGCGACCC AACCTCTGAC ATGGAGTTGC CATCTTCATC AGTCACAGTG
    CCCGCGACTT CTGAACCCAT ACAATCACCC CAAACCAACC CCACTCAGAC ACCACAACTC
    TTTGAACCCC ATGGTGACCC AGCCACTTCA CATTCTGTTG AGCATATACC AGGTGTGCTG
    GAGGGTGTTT GGGAAATACA AGCAGGTGAA TCAAACCAGA TGGTGCACAT GAACATCCTG
    ATCTCCTGTG TGTTAGCTGC GTTTCTGCTT GGTGCATTCA TCGCAGGCAT GGTTGTTTAC
    TGCTATAGAG ATGCGTTTCT TCGCACTCCA CGAAAGATCC AAAAAGATGC AGAGTCGGCA
    CAATCCTGCA CTGATTCCAC TGGCAGCTTT GCCAAGCTTA ACGGTCTGTT TGACAGTCCT
    GTCAAAGAGT ACCAGCCTAA TTTGGATACT CCCAAACTCT ACACCAACCT GTTGAGCAAT
    GGAAGGGAGG TGCCAACCAC TGGAGAAACT AAGACTATGC TCCTGAGTGG GCAGCCACCG
    GAATTAGCAG CACTGCCTAC GCCTGAATCC ACACCTGTGC TGCAACAGAA GAGCCTCCAG
    CCCATTAAGA ACCAGTGGGA GAGAGCTCAT GGAAAGATGA GTGGCTCCCG CAAAGATGCA
    CCACCCAAAA GCCCACAGTA CCTTCCCTCT AGTCCACCTC CCCATTCTGC AATCAATCCA
    CACATACCTA GTGCTGTGGT ACTGCCTAAT GCCACGCATG AAAACAAAGC AGGCTTCAGC
    ACACCAGAGG GGCCTCAAGC AGAAAAGAAG GTCCAGAATA GTGATCAGCA TGGTTCCAAA
    TCCGGACGTA AAGACCAGAG GCGAACAGTG GATGCCAGGA ATACGTTAAA CGACCTCTTG
    AAACATCTAA ATGATAGCAA CCCCAAGGCC ATCATGGTAG AGATGCCCAA ATCTCGACAG
    CACCTAATGC TGGAACCTAT TGTAAGTCCA ACAGAGATTC CACCTAAAGT CCCAAGTCGA
    GAGGCCTCCT TGTATTCTCC GTCTTCCTCG TTACCAAGGA ACAGCCCGAC CAAGAGAGTG
    GATGTGCCGA CAACGCCAAC GTCGCCCACA GGGCATATGG GAACTCTTGA GAGACAGCGG
    TATCACCGAG GCTCTTCCCA GCGACACTCC ATATCCTCGC CGCCCAAAGG GCTGCACTCA
    CCTAGCGGGG CCATAGTGTC CCGGCAACCT AGCATGAACA GAGGTGGGTA CATACCCCCC
    ACTCCTACCC CACCCACTAG ACTAGACTCT CATGGGGTAC CAATGGCAAT GACGCCTTCC
    GTATCCCGAC AGAGCAGCTA CAGTGGGCAT GGATCTTTAC CACGAACATC CATTAAAAGG
    ACAGCATCGC TAAAGCCCGA TGTGCCACCC AAACCCAACG GCTTTGCACC ACAGACTGCA
    CAAATGAGGC CAGTGAACAA GTATAGCTAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23484
    Clone ID Related Accession (Same CDS sequence) XM_005173648.4 , XM_009293805.2 , XM_009293805.3 , XM_005173648.3
    Accession Version XM_009293805.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3213bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product semaphorin-6D isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293805.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTGGC GAGAACTGAC GATTGACCTG CTGATACTCC TGCTGGTGGC GTCCCGTCTG 
    GATGCTGTGA GCTTTCCTGA GGACAACATC CCTTTAGACG TTGTGGACAG ACACTATGCA
    CGGCAGTACC CGGTGTTTCG TGGACGTCCC TCTGGTAACG AATCTCAGCA CAGACTGGAC
    TTTCAGCTGA TGACCCAGAT ACATGATACG CTGTTCATCG CGGGCAGGGA TCAGGTATAT
    TTGGTGAGTC TGAGGGAGTC CTACAGGAAC GAGATCATTC CATACAGGAA GCTGACGTGG
    CGGTCGGGGC AGGCTGACAG AGAGACGTGC GCCATGAAGG GAAAACACAG AGACGAGTGC
    CATAATTTCA TTAAAGTCCT GGTTCCGAGA AATGATGATC TCGTGTTCAT CTGCGGAACA
    AATGGTTTCA ATCCCATGTG CCGTTACTAC AGGTTGGATA ACCTGGAATT TGATGGGGAA
    GAGATCAGCG GTTTGGCCCG ATGCCCGTTT GACGCTAAGC AGACAAACGT TGCCCTGTTT
    GCCGATGGTA AGCTGTACTC TGCTACGGTG GCAGACTTCC TGGCTAGCGA TGCTGTGATT
    TATCGCAGTA TGGGAGATGG TTCTGCCCTT CGCACTATCA AGTATGACTC CAAGTGGTTG
    AAAGAGCCAC ATTTTCTTCA CGCTGTGGAC TACGGGGATT TTGTCTACTT CTTCTTTAGA
    GAAATTGCAG CAGAGCACAA CAACTTGGGA AAGGCGGTTT ACTCCAGAGT GGCTCGGATC
    TGTAAAAATG ACATGGGAGG GTCTCAGCGG GTGCTGGAAA AACACTGGAC ATCTTTTGTT
    AAGGCTCGGC TGAACTGTTC CGTTCCTGGA GAATCGTTTT TCTACTTTGA CGTCCTTCAG
    GCCATTACCG ATATCATCGA CATTAATGGG GTGCCTTCTG TGGTTGGAGT TTTCACCACT
    CAGTTAAACA GTATTCCTGG ATCTGCAGTG TGTGCCTTCT CCATGGTGGA CATTGAGAAA
    GCCTTCCGCG GCCGTTTCAA AGAGCAGAAG ACGCCAGATT CAGTGTGGAC GCCATTTCCA
    GAGGAGAAAC TGCCCAAGCC CAGACCTGGC TGCTGTGCAG GACACGGTTC AGCAGAGTCC
    TACAAAACCT CCATCGAGTT TCCAGACGAG ACCCTTCAAT TCATCAAGTC ACACCCACTC
    ATGGACGCCT CGGTCCCCTC CATTGGAGAC GAACCGTGGT TCACCAAAAC AAGAGTCAGG
    TACAGACTAA CAGCACTTGC AGTTGACAAC ACGGCAGGAC CCCATAAGAA TTACACAGTG
    GTTTTCATCG GCTCAGAAGC AGGCATCGTG CTAAAAGTTC TAGCCAAGAC ATCCCCGCTG
    TCTTTGAATG ACAGCGTCCT GCTGGAGGAA ATTGACGTTT TCAACCAGGC CAAGTGTTTA
    TCCAGCAATG AGGACGATCG GCGAATTTTG TCTTTCCACC TAGATAAAGA CAGTCACACA
    CTTTATGTGG CCTTTTCCAG CTGTGTGGTA AGAATACCAC TCAGCCGCTG CGAACGACAC
    AGTTCCTGTC AAAAGTCTTG TATTGCTTCA AGAGACCCAT ACTGCGGATG GATGTCACAT
    GGAGCTTGTG AACGTATTCC TGCAACTATG CAAAGTGGTT ATGAGCAAGA TGTTGAATAT
    GGCAACACTG CTCACCTCGG GGATTGTCAC GAATTTTTGG CCACTACATC AGCGCCAGAT
    TTCAAATCAT ATGGCGACCC AACCTCTGAC ATGGAGTTGC CATCTTCATC AGTCACAGTG
    CCCGCGACTT CTGAACCCAT ACAATCACCC CAAACCAACC CCACTCAGAC ACCACAACTC
    TTTGAACCCC ATGGTGACCC AGCCACTTCA CATTCTGTTG AGCATATACC AGGTGTGCTG
    GAGGGTGTTT GGGAAATACA AGCAGGTGAA TCAAACCAGA TGGTGCACAT GAACATCCTG
    ATCTCCTGTG TGTTAGCTGC GTTTCTGCTT GGTGCATTCA TCGCAGGCAT GGTTGTTTAC
    TGCTATAGAG ATGCGTTTCT TCGCACTCCA CGAAAGATCC AAAAAGATGC AGAGTCGGCA
    CAATCCTGCA CTGATTCCAC TGGCAGCTTT GCCAAGCTTA ACGGTCTGTT TGACAGTCCT
    GTCAAAGAGT ACCAGCCTAA TTTGGATACT CCCAAACTCT ACACCAACCT GTTGAGCAAT
    GGAAGGGAGG TGCCAACCAC TGGAGAAACT AAGACTATGC TCCTGAGTGG GCAGCCACCG
    GAATTAGCAG CACTGCCTAC GCCTGAATCC ACACCTGTGC TGCAACAGAA GAGCCTCCAG
    CCCATTAAGA ACCAGTGGGA GAGAGCTCAT GGAAAGATGA GTGGCTCCCG CAAAGATGCA
    CCACCCAAAA GCCCACAGTA CCTTCCCTCT AGTCCACCTC CCCATTCTGC AATCAATCCA
    CACATACCTA GTGCTGTGGT ACTGCCTAAT GCCACGCATG AAAACAAAGC AGGCTTCAGC
    ACACCAGAGG GGCCTCAAGC AGAAAAGAAG GTCCAGAATA GTGATCAGCA TGGTTCCAAA
    TCCGGACGTA AAGACCAGAG GCGAACAGTG GATGCCAGGA ATACGTTAAA CGACCTCTTG
    AAACATCTAA ATGATAGCAA CCCCAAGGCC ATCATGGTAG AGATGCCCAA ATCTCGACAG
    CACCTAATGC TGGAACCTAT TGTAAGTCCA ACAGAGATTC CACCTAAAGT CCCAAGTCGA
    GAGGCCTCCT TGTATTCTCC GTCTTCCTCG TTACCAAGGA ACAGCCCGAC CAAGAGAGTG
    GATGTGCCGA CAACGCCAAC GTCGCCCACA GGGCATATGG GAACTCTTGA GAGACAGCGG
    TATCACCGAG GCTCTTCCCA GCGACACTCC ATATCCTCGC CGCCCAAAGG GCTGCACTCA
    CCTAGCGGGG CCATAGTGTC CCGGCAACCT AGCATGAACA GAGGTGGGTA CATACCCCCC
    ACTCCTACCC CACCCACTAG ACTAGACTCT CATGGGGTAC CAATGGCAAT GACGCCTTCC
    GTATCCCGAC AGAGCAGCTA CAGTGGGCAT GGATCTTTAC CACGAACATC CATTAAAAGG
    ACAGCATCGC TAAAGCCCGA TGTGCCACCC AAACCCAACG GCTTTGCACC ACAGACTGCA
    CAAATGAGGC CAGTGAACAA GTATAGCTAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292080.1
    CDS623..3835
    Translation

    Target ORF information:

    RefSeq Version XM_009293805.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D, like (sema6dl), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293805.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTGGC GAGAACTGAC GATTGACCTG CTGATACTCC TGCTGGTGGC GTCCCGTCTG 
    GATGCTGTGA GCTTTCCTGA GGACAACATC CCTTTAGACG TTGTGGACAG ACACTATGCA
    CGGCAGTACC CGGTGTTTCG TGGACGTCCC TCTGGTAACG AATCTCAGCA CAGACTGGAC
    TTTCAGCTGA TGACCCAGAT ACATGATACG CTGTTCATCG CGGGCAGGGA TCAGGTATAT
    TTGGTGAGTC TGAGGGAGTC CTACAGGAAC GAGATCATTC CATACAGGAA GCTGACGTGG
    CGGTCGGGGC AGGCTGACAG AGAGACGTGC GCCATGAAGG GAAAACACAG AGACGAGTGC
    CATAATTTCA TTAAAGTCCT GGTTCCGAGA AATGATGATC TCGTGTTCAT CTGCGGAACA
    AATGGTTTCA ATCCCATGTG CCGTTACTAC AGGTTGGATA ACCTGGAATT TGATGGGGAA
    GAGATCAGCG GTTTGGCCCG ATGCCCGTTT GACGCTAAGC AGACAAACGT TGCCCTGTTT
    GCCGATGGTA AGCTGTACTC TGCTACGGTG GCAGACTTCC TGGCTAGCGA TGCTGTGATT
    TATCGCAGTA TGGGAGATGG TTCTGCCCTT CGCACTATCA AGTATGACTC CAAGTGGTTG
    AAAGAGCCAC ATTTTCTTCA CGCTGTGGAC TACGGGGATT TTGTCTACTT CTTCTTTAGA
    GAAATTGCAG CAGAGCACAA CAACTTGGGA AAGGCGGTTT ACTCCAGAGT GGCTCGGATC
    TGTAAAAATG ACATGGGAGG GTCTCAGCGG GTGCTGGAAA AACACTGGAC ATCTTTTGTT
    AAGGCTCGGC TGAACTGTTC CGTTCCTGGA GAATCGTTTT TCTACTTTGA CGTCCTTCAG
    GCCATTACCG ATATCATCGA CATTAATGGG GTGCCTTCTG TGGTTGGAGT TTTCACCACT
    CAGTTAAACA GTATTCCTGG ATCTGCAGTG TGTGCCTTCT CCATGGTGGA CATTGAGAAA
    GCCTTCCGCG GCCGTTTCAA AGAGCAGAAG ACGCCAGATT CAGTGTGGAC GCCATTTCCA
    GAGGAGAAAC TGCCCAAGCC CAGACCTGGC TGCTGTGCAG GACACGGTTC AGCAGAGTCC
    TACAAAACCT CCATCGAGTT TCCAGACGAG ACCCTTCAAT TCATCAAGTC ACACCCACTC
    ATGGACGCCT CGGTCCCCTC CATTGGAGAC GAACCGTGGT TCACCAAAAC AAGAGTCAGG
    TACAGACTAA CAGCACTTGC AGTTGACAAC ACGGCAGGAC CCCATAAGAA TTACACAGTG
    GTTTTCATCG GCTCAGAAGC AGGCATCGTG CTAAAAGTTC TAGCCAAGAC ATCCCCGCTG
    TCTTTGAATG ACAGCGTCCT GCTGGAGGAA ATTGACGTTT TCAACCAGGC CAAGTGTTTA
    TCCAGCAATG AGGACGATCG GCGAATTTTG TCTTTCCACC TAGATAAAGA CAGTCACACA
    CTTTATGTGG CCTTTTCCAG CTGTGTGGTA AGAATACCAC TCAGCCGCTG CGAACGACAC
    AGTTCCTGTC AAAAGTCTTG TATTGCTTCA AGAGACCCAT ACTGCGGATG GATGTCACAT
    GGAGCTTGTG AACGTATTCC TGCAACTATG CAAAGTGGTT ATGAGCAAGA TGTTGAATAT
    GGCAACACTG CTCACCTCGG GGATTGTCAC GAATTTTTGG CCACTACATC AGCGCCAGAT
    TTCAAATCAT ATGGCGACCC AACCTCTGAC ATGGAGTTGC CATCTTCATC AGTCACAGTG
    CCCGCGACTT CTGAACCCAT ACAATCACCC CAAACCAACC CCACTCAGAC ACCACAACTC
    TTTGAACCCC ATGGTGACCC AGCCACTTCA CATTCTGTTG AGCATATACC AGGTGTGCTG
    GAGGGTGTTT GGGAAATACA AGCAGGTGAA TCAAACCAGA TGGTGCACAT GAACATCCTG
    ATCTCCTGTG TGTTAGCTGC GTTTCTGCTT GGTGCATTCA TCGCAGGCAT GGTTGTTTAC
    TGCTATAGAG ATGCGTTTCT TCGCACTCCA CGAAAGATCC AAAAAGATGC AGAGTCGGCA
    CAATCCTGCA CTGATTCCAC TGGCAGCTTT GCCAAGCTTA ACGGTCTGTT TGACAGTCCT
    GTCAAAGAGT ACCAGCCTAA TTTGGATACT CCCAAACTCT ACACCAACCT GTTGAGCAAT
    GGAAGGGAGG TGCCAACCAC TGGAGAAACT AAGACTATGC TCCTGAGTGG GCAGCCACCG
    GAATTAGCAG CACTGCCTAC GCCTGAATCC ACACCTGTGC TGCAACAGAA GAGCCTCCAG
    CCCATTAAGA ACCAGTGGGA GAGAGCTCAT GGAAAGATGA GTGGCTCCCG CAAAGATGCA
    CCACCCAAAA GCCCACAGTA CCTTCCCTCT AGTCCACCTC CCCATTCTGC AATCAATCCA
    CACATACCTA GTGCTGTGGT ACTGCCTAAT GCCACGCATG AAAACAAAGC AGGCTTCAGC
    ACACCAGAGG GGCCTCAAGC AGAAAAGAAG GTCCAGAATA GTGATCAGCA TGGTTCCAAA
    TCCGGACGTA AAGACCAGAG GCGAACAGTG GATGCCAGGA ATACGTTAAA CGACCTCTTG
    AAACATCTAA ATGATAGCAA CCCCAAGGCC ATCATGGTAG AGATGCCCAA ATCTCGACAG
    CACCTAATGC TGGAACCTAT TGTAAGTCCA ACAGAGATTC CACCTAAAGT CCCAAGTCGA
    GAGGCCTCCT TGTATTCTCC GTCTTCCTCG TTACCAAGGA ACAGCCCGAC CAAGAGAGTG
    GATGTGCCGA CAACGCCAAC GTCGCCCACA GGGCATATGG GAACTCTTGA GAGACAGCGG
    TATCACCGAG GCTCTTCCCA GCGACACTCC ATATCCTCGC CGCCCAAAGG GCTGCACTCA
    CCTAGCGGGG CCATAGTGTC CCGGCAACCT AGCATGAACA GAGGTGGGTA CATACCCCCC
    ACTCCTACCC CACCCACTAG ACTAGACTCT CATGGGGTAC CAATGGCAAT GACGCCTTCC
    GTATCCCGAC AGAGCAGCTA CAGTGGGCAT GGATCTTTAC CACGAACATC CATTAAAAGG
    ACAGCATCGC TAAAGCCCGA TGTGCCACCC AAACCCAACG GCTTTGCACC ACAGACTGCA
    CAAATGAGGC CAGTGAACAA GTATAGCTAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23484
    Clone ID Related Accession (Same CDS sequence) XM_005173648.4 , XM_009293805.2 , XM_009293805.3 , XM_005173648.3
    Accession Version XM_009293805.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3213bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product semaphorin-6D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293805.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTGGC GAGAACTGAC GATTGACCTG CTGATACTCC TGCTGGTGGC GTCCCGTCTG 
    GATGCTGTGA GCTTTCCTGA GGACAACATC CCTTTAGACG TTGTGGACAG ACACTATGCA
    CGGCAGTACC CGGTGTTTCG TGGACGTCCC TCTGGTAACG AATCTCAGCA CAGACTGGAC
    TTTCAGCTGA TGACCCAGAT ACATGATACG CTGTTCATCG CGGGCAGGGA TCAGGTATAT
    TTGGTGAGTC TGAGGGAGTC CTACAGGAAC GAGATCATTC CATACAGGAA GCTGACGTGG
    CGGTCGGGGC AGGCTGACAG AGAGACGTGC GCCATGAAGG GAAAACACAG AGACGAGTGC
    CATAATTTCA TTAAAGTCCT GGTTCCGAGA AATGATGATC TCGTGTTCAT CTGCGGAACA
    AATGGTTTCA ATCCCATGTG CCGTTACTAC AGGTTGGATA ACCTGGAATT TGATGGGGAA
    GAGATCAGCG GTTTGGCCCG ATGCCCGTTT GACGCTAAGC AGACAAACGT TGCCCTGTTT
    GCCGATGGTA AGCTGTACTC TGCTACGGTG GCAGACTTCC TGGCTAGCGA TGCTGTGATT
    TATCGCAGTA TGGGAGATGG TTCTGCCCTT CGCACTATCA AGTATGACTC CAAGTGGTTG
    AAAGAGCCAC ATTTTCTTCA CGCTGTGGAC TACGGGGATT TTGTCTACTT CTTCTTTAGA
    GAAATTGCAG CAGAGCACAA CAACTTGGGA AAGGCGGTTT ACTCCAGAGT GGCTCGGATC
    TGTAAAAATG ACATGGGAGG GTCTCAGCGG GTGCTGGAAA AACACTGGAC ATCTTTTGTT
    AAGGCTCGGC TGAACTGTTC CGTTCCTGGA GAATCGTTTT TCTACTTTGA CGTCCTTCAG
    GCCATTACCG ATATCATCGA CATTAATGGG GTGCCTTCTG TGGTTGGAGT TTTCACCACT
    CAGTTAAACA GTATTCCTGG ATCTGCAGTG TGTGCCTTCT CCATGGTGGA CATTGAGAAA
    GCCTTCCGCG GCCGTTTCAA AGAGCAGAAG ACGCCAGATT CAGTGTGGAC GCCATTTCCA
    GAGGAGAAAC TGCCCAAGCC CAGACCTGGC TGCTGTGCAG GACACGGTTC AGCAGAGTCC
    TACAAAACCT CCATCGAGTT TCCAGACGAG ACCCTTCAAT TCATCAAGTC ACACCCACTC
    ATGGACGCCT CGGTCCCCTC CATTGGAGAC GAACCGTGGT TCACCAAAAC AAGAGTCAGG
    TACAGACTAA CAGCACTTGC AGTTGACAAC ACGGCAGGAC CCCATAAGAA TTACACAGTG
    GTTTTCATCG GCTCAGAAGC AGGCATCGTG CTAAAAGTTC TAGCCAAGAC ATCCCCGCTG
    TCTTTGAATG ACAGCGTCCT GCTGGAGGAA ATTGACGTTT TCAACCAGGC CAAGTGTTTA
    TCCAGCAATG AGGACGATCG GCGAATTTTG TCTTTCCACC TAGATAAAGA CAGTCACACA
    CTTTATGTGG CCTTTTCCAG CTGTGTGGTA AGAATACCAC TCAGCCGCTG CGAACGACAC
    AGTTCCTGTC AAAAGTCTTG TATTGCTTCA AGAGACCCAT ACTGCGGATG GATGTCACAT
    GGAGCTTGTG AACGTATTCC TGCAACTATG CAAAGTGGTT ATGAGCAAGA TGTTGAATAT
    GGCAACACTG CTCACCTCGG GGATTGTCAC GAATTTTTGG CCACTACATC AGCGCCAGAT
    TTCAAATCAT ATGGCGACCC AACCTCTGAC ATGGAGTTGC CATCTTCATC AGTCACAGTG
    CCCGCGACTT CTGAACCCAT ACAATCACCC CAAACCAACC CCACTCAGAC ACCACAACTC
    TTTGAACCCC ATGGTGACCC AGCCACTTCA CATTCTGTTG AGCATATACC AGGTGTGCTG
    GAGGGTGTTT GGGAAATACA AGCAGGTGAA TCAAACCAGA TGGTGCACAT GAACATCCTG
    ATCTCCTGTG TGTTAGCTGC GTTTCTGCTT GGTGCATTCA TCGCAGGCAT GGTTGTTTAC
    TGCTATAGAG ATGCGTTTCT TCGCACTCCA CGAAAGATCC AAAAAGATGC AGAGTCGGCA
    CAATCCTGCA CTGATTCCAC TGGCAGCTTT GCCAAGCTTA ACGGTCTGTT TGACAGTCCT
    GTCAAAGAGT ACCAGCCTAA TTTGGATACT CCCAAACTCT ACACCAACCT GTTGAGCAAT
    GGAAGGGAGG TGCCAACCAC TGGAGAAACT AAGACTATGC TCCTGAGTGG GCAGCCACCG
    GAATTAGCAG CACTGCCTAC GCCTGAATCC ACACCTGTGC TGCAACAGAA GAGCCTCCAG
    CCCATTAAGA ACCAGTGGGA GAGAGCTCAT GGAAAGATGA GTGGCTCCCG CAAAGATGCA
    CCACCCAAAA GCCCACAGTA CCTTCCCTCT AGTCCACCTC CCCATTCTGC AATCAATCCA
    CACATACCTA GTGCTGTGGT ACTGCCTAAT GCCACGCATG AAAACAAAGC AGGCTTCAGC
    ACACCAGAGG GGCCTCAAGC AGAAAAGAAG GTCCAGAATA GTGATCAGCA TGGTTCCAAA
    TCCGGACGTA AAGACCAGAG GCGAACAGTG GATGCCAGGA ATACGTTAAA CGACCTCTTG
    AAACATCTAA ATGATAGCAA CCCCAAGGCC ATCATGGTAG AGATGCCCAA ATCTCGACAG
    CACCTAATGC TGGAACCTAT TGTAAGTCCA ACAGAGATTC CACCTAAAGT CCCAAGTCGA
    GAGGCCTCCT TGTATTCTCC GTCTTCCTCG TTACCAAGGA ACAGCCCGAC CAAGAGAGTG
    GATGTGCCGA CAACGCCAAC GTCGCCCACA GGGCATATGG GAACTCTTGA GAGACAGCGG
    TATCACCGAG GCTCTTCCCA GCGACACTCC ATATCCTCGC CGCCCAAAGG GCTGCACTCA
    CCTAGCGGGG CCATAGTGTC CCGGCAACCT AGCATGAACA GAGGTGGGTA CATACCCCCC
    ACTCCTACCC CACCCACTAG ACTAGACTCT CATGGGGTAC CAATGGCAAT GACGCCTTCC
    GTATCCCGAC AGAGCAGCTA CAGTGGGCAT GGATCTTTAC CACGAACATC CATTAAAAGG
    ACAGCATCGC TAAAGCCCGA TGTGCCACCC AAACCCAACG GCTTTGCACC ACAGACTGCA
    CAAATGAGGC CAGTGAACAA GTATAGCTAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292080.1
    CDS610..3822
    Translation

    Target ORF information:

    RefSeq Version XM_009293805.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D, like (sema6dl), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293805.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTGGC GAGAACTGAC GATTGACCTG CTGATACTCC TGCTGGTGGC GTCCCGTCTG 
    GATGCTGTGA GCTTTCCTGA GGACAACATC CCTTTAGACG TTGTGGACAG ACACTATGCA
    CGGCAGTACC CGGTGTTTCG TGGACGTCCC TCTGGTAACG AATCTCAGCA CAGACTGGAC
    TTTCAGCTGA TGACCCAGAT ACATGATACG CTGTTCATCG CGGGCAGGGA TCAGGTATAT
    TTGGTGAGTC TGAGGGAGTC CTACAGGAAC GAGATCATTC CATACAGGAA GCTGACGTGG
    CGGTCGGGGC AGGCTGACAG AGAGACGTGC GCCATGAAGG GAAAACACAG AGACGAGTGC
    CATAATTTCA TTAAAGTCCT GGTTCCGAGA AATGATGATC TCGTGTTCAT CTGCGGAACA
    AATGGTTTCA ATCCCATGTG CCGTTACTAC AGGTTGGATA ACCTGGAATT TGATGGGGAA
    GAGATCAGCG GTTTGGCCCG ATGCCCGTTT GACGCTAAGC AGACAAACGT TGCCCTGTTT
    GCCGATGGTA AGCTGTACTC TGCTACGGTG GCAGACTTCC TGGCTAGCGA TGCTGTGATT
    TATCGCAGTA TGGGAGATGG TTCTGCCCTT CGCACTATCA AGTATGACTC CAAGTGGTTG
    AAAGAGCCAC ATTTTCTTCA CGCTGTGGAC TACGGGGATT TTGTCTACTT CTTCTTTAGA
    GAAATTGCAG CAGAGCACAA CAACTTGGGA AAGGCGGTTT ACTCCAGAGT GGCTCGGATC
    TGTAAAAATG ACATGGGAGG GTCTCAGCGG GTGCTGGAAA AACACTGGAC ATCTTTTGTT
    AAGGCTCGGC TGAACTGTTC CGTTCCTGGA GAATCGTTTT TCTACTTTGA CGTCCTTCAG
    GCCATTACCG ATATCATCGA CATTAATGGG GTGCCTTCTG TGGTTGGAGT TTTCACCACT
    CAGTTAAACA GTATTCCTGG ATCTGCAGTG TGTGCCTTCT CCATGGTGGA CATTGAGAAA
    GCCTTCCGCG GCCGTTTCAA AGAGCAGAAG ACGCCAGATT CAGTGTGGAC GCCATTTCCA
    GAGGAGAAAC TGCCCAAGCC CAGACCTGGC TGCTGTGCAG GACACGGTTC AGCAGAGTCC
    TACAAAACCT CCATCGAGTT TCCAGACGAG ACCCTTCAAT TCATCAAGTC ACACCCACTC
    ATGGACGCCT CGGTCCCCTC CATTGGAGAC GAACCGTGGT TCACCAAAAC AAGAGTCAGG
    TACAGACTAA CAGCACTTGC AGTTGACAAC ACGGCAGGAC CCCATAAGAA TTACACAGTG
    GTTTTCATCG GCTCAGAAGC AGGCATCGTG CTAAAAGTTC TAGCCAAGAC ATCCCCGCTG
    TCTTTGAATG ACAGCGTCCT GCTGGAGGAA ATTGACGTTT TCAACCAGGC CAAGTGTTTA
    TCCAGCAATG AGGACGATCG GCGAATTTTG TCTTTCCACC TAGATAAAGA CAGTCACACA
    CTTTATGTGG CCTTTTCCAG CTGTGTGGTA AGAATACCAC TCAGCCGCTG CGAACGACAC
    AGTTCCTGTC AAAAGTCTTG TATTGCTTCA AGAGACCCAT ACTGCGGATG GATGTCACAT
    GGAGCTTGTG AACGTATTCC TGCAACTATG CAAAGTGGTT ATGAGCAAGA TGTTGAATAT
    GGCAACACTG CTCACCTCGG GGATTGTCAC GAATTTTTGG CCACTACATC AGCGCCAGAT
    TTCAAATCAT ATGGCGACCC AACCTCTGAC ATGGAGTTGC CATCTTCATC AGTCACAGTG
    CCCGCGACTT CTGAACCCAT ACAATCACCC CAAACCAACC CCACTCAGAC ACCACAACTC
    TTTGAACCCC ATGGTGACCC AGCCACTTCA CATTCTGTTG AGCATATACC AGGTGTGCTG
    GAGGGTGTTT GGGAAATACA AGCAGGTGAA TCAAACCAGA TGGTGCACAT GAACATCCTG
    ATCTCCTGTG TGTTAGCTGC GTTTCTGCTT GGTGCATTCA TCGCAGGCAT GGTTGTTTAC
    TGCTATAGAG ATGCGTTTCT TCGCACTCCA CGAAAGATCC AAAAAGATGC AGAGTCGGCA
    CAATCCTGCA CTGATTCCAC TGGCAGCTTT GCCAAGCTTA ACGGTCTGTT TGACAGTCCT
    GTCAAAGAGT ACCAGCCTAA TTTGGATACT CCCAAACTCT ACACCAACCT GTTGAGCAAT
    GGAAGGGAGG TGCCAACCAC TGGAGAAACT AAGACTATGC TCCTGAGTGG GCAGCCACCG
    GAATTAGCAG CACTGCCTAC GCCTGAATCC ACACCTGTGC TGCAACAGAA GAGCCTCCAG
    CCCATTAAGA ACCAGTGGGA GAGAGCTCAT GGAAAGATGA GTGGCTCCCG CAAAGATGCA
    CCACCCAAAA GCCCACAGTA CCTTCCCTCT AGTCCACCTC CCCATTCTGC AATCAATCCA
    CACATACCTA GTGCTGTGGT ACTGCCTAAT GCCACGCATG AAAACAAAGC AGGCTTCAGC
    ACACCAGAGG GGCCTCAAGC AGAAAAGAAG GTCCAGAATA GTGATCAGCA TGGTTCCAAA
    TCCGGACGTA AAGACCAGAG GCGAACAGTG GATGCCAGGA ATACGTTAAA CGACCTCTTG
    AAACATCTAA ATGATAGCAA CCCCAAGGCC ATCATGGTAG AGATGCCCAA ATCTCGACAG
    CACCTAATGC TGGAACCTAT TGTAAGTCCA ACAGAGATTC CACCTAAAGT CCCAAGTCGA
    GAGGCCTCCT TGTATTCTCC GTCTTCCTCG TTACCAAGGA ACAGCCCGAC CAAGAGAGTG
    GATGTGCCGA CAACGCCAAC GTCGCCCACA GGGCATATGG GAACTCTTGA GAGACAGCGG
    TATCACCGAG GCTCTTCCCA GCGACACTCC ATATCCTCGC CGCCCAAAGG GCTGCACTCA
    CCTAGCGGGG CCATAGTGTC CCGGCAACCT AGCATGAACA GAGGTGGGTA CATACCCCCC
    ACTCCTACCC CACCCACTAG ACTAGACTCT CATGGGGTAC CAATGGCAAT GACGCCTTCC
    GTATCCCGAC AGAGCAGCTA CAGTGGGCAT GGATCTTTAC CACGAACATC CATTAAAAGG
    ACAGCATCGC TAAAGCCCGA TGTGCCACCC AAACCCAACG GCTTTGCACC ACAGACTGCA
    CAAATGAGGC CAGTGAACAA GTATAGCTAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23484
    Clone ID Related Accession (Same CDS sequence) XM_005173648.4 , XM_009293805.2 , XM_009293805.3 , XM_005173648.3
    Accession Version XM_005173648.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3213bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product semaphorin-6D isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173648.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTGGC GAGAACTGAC GATTGACCTG CTGATACTCC TGCTGGTGGC GTCCCGTCTG 
    GATGCTGTGA GCTTTCCTGA GGACAACATC CCTTTAGACG TTGTGGACAG ACACTATGCA
    CGGCAGTACC CGGTGTTTCG TGGACGTCCC TCTGGTAACG AATCTCAGCA CAGACTGGAC
    TTTCAGCTGA TGACCCAGAT ACATGATACG CTGTTCATCG CGGGCAGGGA TCAGGTATAT
    TTGGTGAGTC TGAGGGAGTC CTACAGGAAC GAGATCATTC CATACAGGAA GCTGACGTGG
    CGGTCGGGGC AGGCTGACAG AGAGACGTGC GCCATGAAGG GAAAACACAG AGACGAGTGC
    CATAATTTCA TTAAAGTCCT GGTTCCGAGA AATGATGATC TCGTGTTCAT CTGCGGAACA
    AATGGTTTCA ATCCCATGTG CCGTTACTAC AGGTTGGATA ACCTGGAATT TGATGGGGAA
    GAGATCAGCG GTTTGGCCCG ATGCCCGTTT GACGCTAAGC AGACAAACGT TGCCCTGTTT
    GCCGATGGTA AGCTGTACTC TGCTACGGTG GCAGACTTCC TGGCTAGCGA TGCTGTGATT
    TATCGCAGTA TGGGAGATGG TTCTGCCCTT CGCACTATCA AGTATGACTC CAAGTGGTTG
    AAAGAGCCAC ATTTTCTTCA CGCTGTGGAC TACGGGGATT TTGTCTACTT CTTCTTTAGA
    GAAATTGCAG CAGAGCACAA CAACTTGGGA AAGGCGGTTT ACTCCAGAGT GGCTCGGATC
    TGTAAAAATG ACATGGGAGG GTCTCAGCGG GTGCTGGAAA AACACTGGAC ATCTTTTGTT
    AAGGCTCGGC TGAACTGTTC CGTTCCTGGA GAATCGTTTT TCTACTTTGA CGTCCTTCAG
    GCCATTACCG ATATCATCGA CATTAATGGG GTGCCTTCTG TGGTTGGAGT TTTCACCACT
    CAGTTAAACA GTATTCCTGG ATCTGCAGTG TGTGCCTTCT CCATGGTGGA CATTGAGAAA
    GCCTTCCGCG GCCGTTTCAA AGAGCAGAAG ACGCCAGATT CAGTGTGGAC GCCATTTCCA
    GAGGAGAAAC TGCCCAAGCC CAGACCTGGC TGCTGTGCAG GACACGGTTC AGCAGAGTCC
    TACAAAACCT CCATCGAGTT TCCAGACGAG ACCCTTCAAT TCATCAAGTC ACACCCACTC
    ATGGACGCCT CGGTCCCCTC CATTGGAGAC GAACCGTGGT TCACCAAAAC AAGAGTCAGG
    TACAGACTAA CAGCACTTGC AGTTGACAAC ACGGCAGGAC CCCATAAGAA TTACACAGTG
    GTTTTCATCG GCTCAGAAGC AGGCATCGTG CTAAAAGTTC TAGCCAAGAC ATCCCCGCTG
    TCTTTGAATG ACAGCGTCCT GCTGGAGGAA ATTGACGTTT TCAACCAGGC CAAGTGTTTA
    TCCAGCAATG AGGACGATCG GCGAATTTTG TCTTTCCACC TAGATAAAGA CAGTCACACA
    CTTTATGTGG CCTTTTCCAG CTGTGTGGTA AGAATACCAC TCAGCCGCTG CGAACGACAC
    AGTTCCTGTC AAAAGTCTTG TATTGCTTCA AGAGACCCAT ACTGCGGATG GATGTCACAT
    GGAGCTTGTG AACGTATTCC TGCAACTATG CAAAGTGGTT ATGAGCAAGA TGTTGAATAT
    GGCAACACTG CTCACCTCGG GGATTGTCAC GAATTTTTGG CCACTACATC AGCGCCAGAT
    TTCAAATCAT ATGGCGACCC AACCTCTGAC ATGGAGTTGC CATCTTCATC AGTCACAGTG
    CCCGCGACTT CTGAACCCAT ACAATCACCC CAAACCAACC CCACTCAGAC ACCACAACTC
    TTTGAACCCC ATGGTGACCC AGCCACTTCA CATTCTGTTG AGCATATACC AGGTGTGCTG
    GAGGGTGTTT GGGAAATACA AGCAGGTGAA TCAAACCAGA TGGTGCACAT GAACATCCTG
    ATCTCCTGTG TGTTAGCTGC GTTTCTGCTT GGTGCATTCA TCGCAGGCAT GGTTGTTTAC
    TGCTATAGAG ATGCGTTTCT TCGCACTCCA CGAAAGATCC AAAAAGATGC AGAGTCGGCA
    CAATCCTGCA CTGATTCCAC TGGCAGCTTT GCCAAGCTTA ACGGTCTGTT TGACAGTCCT
    GTCAAAGAGT ACCAGCCTAA TTTGGATACT CCCAAACTCT ACACCAACCT GTTGAGCAAT
    GGAAGGGAGG TGCCAACCAC TGGAGAAACT AAGACTATGC TCCTGAGTGG GCAGCCACCG
    GAATTAGCAG CACTGCCTAC GCCTGAATCC ACACCTGTGC TGCAACAGAA GAGCCTCCAG
    CCCATTAAGA ACCAGTGGGA GAGAGCTCAT GGAAAGATGA GTGGCTCCCG CAAAGATGCA
    CCACCCAAAA GCCCACAGTA CCTTCCCTCT AGTCCACCTC CCCATTCTGC AATCAATCCA
    CACATACCTA GTGCTGTGGT ACTGCCTAAT GCCACGCATG AAAACAAAGC AGGCTTCAGC
    ACACCAGAGG GGCCTCAAGC AGAAAAGAAG GTCCAGAATA GTGATCAGCA TGGTTCCAAA
    TCCGGACGTA AAGACCAGAG GCGAACAGTG GATGCCAGGA ATACGTTAAA CGACCTCTTG
    AAACATCTAA ATGATAGCAA CCCCAAGGCC ATCATGGTAG AGATGCCCAA ATCTCGACAG
    CACCTAATGC TGGAACCTAT TGTAAGTCCA ACAGAGATTC CACCTAAAGT CCCAAGTCGA
    GAGGCCTCCT TGTATTCTCC GTCTTCCTCG TTACCAAGGA ACAGCCCGAC CAAGAGAGTG
    GATGTGCCGA CAACGCCAAC GTCGCCCACA GGGCATATGG GAACTCTTGA GAGACAGCGG
    TATCACCGAG GCTCTTCCCA GCGACACTCC ATATCCTCGC CGCCCAAAGG GCTGCACTCA
    CCTAGCGGGG CCATAGTGTC CCGGCAACCT AGCATGAACA GAGGTGGGTA CATACCCCCC
    ACTCCTACCC CACCCACTAG ACTAGACTCT CATGGGGTAC CAATGGCAAT GACGCCTTCC
    GTATCCCGAC AGAGCAGCTA CAGTGGGCAT GGATCTTTAC CACGAACATC CATTAAAAGG
    ACAGCATCGC TAAAGCCCGA TGTGCCACCC AAACCCAACG GCTTTGCACC ACAGACTGCA
    CAAATGAGGC CAGTGAACAA GTATAGCTAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173705.1
    CDS443..3655
    Translation

    Target ORF information:

    RefSeq Version XM_005173648.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D, like (sema6dl), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173648.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTGGC GAGAACTGAC GATTGACCTG CTGATACTCC TGCTGGTGGC GTCCCGTCTG 
    GATGCTGTGA GCTTTCCTGA GGACAACATC CCTTTAGACG TTGTGGACAG ACACTATGCA
    CGGCAGTACC CGGTGTTTCG TGGACGTCCC TCTGGTAACG AATCTCAGCA CAGACTGGAC
    TTTCAGCTGA TGACCCAGAT ACATGATACG CTGTTCATCG CGGGCAGGGA TCAGGTATAT
    TTGGTGAGTC TGAGGGAGTC CTACAGGAAC GAGATCATTC CATACAGGAA GCTGACGTGG
    CGGTCGGGGC AGGCTGACAG AGAGACGTGC GCCATGAAGG GAAAACACAG AGACGAGTGC
    CATAATTTCA TTAAAGTCCT GGTTCCGAGA AATGATGATC TCGTGTTCAT CTGCGGAACA
    AATGGTTTCA ATCCCATGTG CCGTTACTAC AGGTTGGATA ACCTGGAATT TGATGGGGAA
    GAGATCAGCG GTTTGGCCCG ATGCCCGTTT GACGCTAAGC AGACAAACGT TGCCCTGTTT
    GCCGATGGTA AGCTGTACTC TGCTACGGTG GCAGACTTCC TGGCTAGCGA TGCTGTGATT
    TATCGCAGTA TGGGAGATGG TTCTGCCCTT CGCACTATCA AGTATGACTC CAAGTGGTTG
    AAAGAGCCAC ATTTTCTTCA CGCTGTGGAC TACGGGGATT TTGTCTACTT CTTCTTTAGA
    GAAATTGCAG CAGAGCACAA CAACTTGGGA AAGGCGGTTT ACTCCAGAGT GGCTCGGATC
    TGTAAAAATG ACATGGGAGG GTCTCAGCGG GTGCTGGAAA AACACTGGAC ATCTTTTGTT
    AAGGCTCGGC TGAACTGTTC CGTTCCTGGA GAATCGTTTT TCTACTTTGA CGTCCTTCAG
    GCCATTACCG ATATCATCGA CATTAATGGG GTGCCTTCTG TGGTTGGAGT TTTCACCACT
    CAGTTAAACA GTATTCCTGG ATCTGCAGTG TGTGCCTTCT CCATGGTGGA CATTGAGAAA
    GCCTTCCGCG GCCGTTTCAA AGAGCAGAAG ACGCCAGATT CAGTGTGGAC GCCATTTCCA
    GAGGAGAAAC TGCCCAAGCC CAGACCTGGC TGCTGTGCAG GACACGGTTC AGCAGAGTCC
    TACAAAACCT CCATCGAGTT TCCAGACGAG ACCCTTCAAT TCATCAAGTC ACACCCACTC
    ATGGACGCCT CGGTCCCCTC CATTGGAGAC GAACCGTGGT TCACCAAAAC AAGAGTCAGG
    TACAGACTAA CAGCACTTGC AGTTGACAAC ACGGCAGGAC CCCATAAGAA TTACACAGTG
    GTTTTCATCG GCTCAGAAGC AGGCATCGTG CTAAAAGTTC TAGCCAAGAC ATCCCCGCTG
    TCTTTGAATG ACAGCGTCCT GCTGGAGGAA ATTGACGTTT TCAACCAGGC CAAGTGTTTA
    TCCAGCAATG AGGACGATCG GCGAATTTTG TCTTTCCACC TAGATAAAGA CAGTCACACA
    CTTTATGTGG CCTTTTCCAG CTGTGTGGTA AGAATACCAC TCAGCCGCTG CGAACGACAC
    AGTTCCTGTC AAAAGTCTTG TATTGCTTCA AGAGACCCAT ACTGCGGATG GATGTCACAT
    GGAGCTTGTG AACGTATTCC TGCAACTATG CAAAGTGGTT ATGAGCAAGA TGTTGAATAT
    GGCAACACTG CTCACCTCGG GGATTGTCAC GAATTTTTGG CCACTACATC AGCGCCAGAT
    TTCAAATCAT ATGGCGACCC AACCTCTGAC ATGGAGTTGC CATCTTCATC AGTCACAGTG
    CCCGCGACTT CTGAACCCAT ACAATCACCC CAAACCAACC CCACTCAGAC ACCACAACTC
    TTTGAACCCC ATGGTGACCC AGCCACTTCA CATTCTGTTG AGCATATACC AGGTGTGCTG
    GAGGGTGTTT GGGAAATACA AGCAGGTGAA TCAAACCAGA TGGTGCACAT GAACATCCTG
    ATCTCCTGTG TGTTAGCTGC GTTTCTGCTT GGTGCATTCA TCGCAGGCAT GGTTGTTTAC
    TGCTATAGAG ATGCGTTTCT TCGCACTCCA CGAAAGATCC AAAAAGATGC AGAGTCGGCA
    CAATCCTGCA CTGATTCCAC TGGCAGCTTT GCCAAGCTTA ACGGTCTGTT TGACAGTCCT
    GTCAAAGAGT ACCAGCCTAA TTTGGATACT CCCAAACTCT ACACCAACCT GTTGAGCAAT
    GGAAGGGAGG TGCCAACCAC TGGAGAAACT AAGACTATGC TCCTGAGTGG GCAGCCACCG
    GAATTAGCAG CACTGCCTAC GCCTGAATCC ACACCTGTGC TGCAACAGAA GAGCCTCCAG
    CCCATTAAGA ACCAGTGGGA GAGAGCTCAT GGAAAGATGA GTGGCTCCCG CAAAGATGCA
    CCACCCAAAA GCCCACAGTA CCTTCCCTCT AGTCCACCTC CCCATTCTGC AATCAATCCA
    CACATACCTA GTGCTGTGGT ACTGCCTAAT GCCACGCATG AAAACAAAGC AGGCTTCAGC
    ACACCAGAGG GGCCTCAAGC AGAAAAGAAG GTCCAGAATA GTGATCAGCA TGGTTCCAAA
    TCCGGACGTA AAGACCAGAG GCGAACAGTG GATGCCAGGA ATACGTTAAA CGACCTCTTG
    AAACATCTAA ATGATAGCAA CCCCAAGGCC ATCATGGTAG AGATGCCCAA ATCTCGACAG
    CACCTAATGC TGGAACCTAT TGTAAGTCCA ACAGAGATTC CACCTAAAGT CCCAAGTCGA
    GAGGCCTCCT TGTATTCTCC GTCTTCCTCG TTACCAAGGA ACAGCCCGAC CAAGAGAGTG
    GATGTGCCGA CAACGCCAAC GTCGCCCACA GGGCATATGG GAACTCTTGA GAGACAGCGG
    TATCACCGAG GCTCTTCCCA GCGACACTCC ATATCCTCGC CGCCCAAAGG GCTGCACTCA
    CCTAGCGGGG CCATAGTGTC CCGGCAACCT AGCATGAACA GAGGTGGGTA CATACCCCCC
    ACTCCTACCC CACCCACTAG ACTAGACTCT CATGGGGTAC CAATGGCAAT GACGCCTTCC
    GTATCCCGAC AGAGCAGCTA CAGTGGGCAT GGATCTTTAC CACGAACATC CATTAAAAGG
    ACAGCATCGC TAAAGCCCGA TGTGCCACCC AAACCCAACG GCTTTGCACC ACAGACTGCA
    CAAATGAGGC CAGTGAACAA GTATAGCTAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23485
    Clone ID Related Accession (Same CDS sequence) XM_005173649.3 , XM_005173649.4
    Accession Version XM_005173649.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3156bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product semaphorin-6D isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173649.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGTGGC GAGAACTGAC GATTGACCTG CTGATACTCC TGCTGGTGGC GTCCCGTCTG 
    GATGCTGTGA GCTTTCCTGA GGACAACATC CCTTTAGACG TTGTGGACAG ACACTATGCA
    CGGCAGTACC CGGTGTTTCG TGGACGTCCC TCTGGTAACG AATCTCAGCA CAGACTGGAC
    TTTCAGCTGA TGACCCAGAT ACATGATACG CTGTTCATCG CGGGCAGGGA TCAGGTATAT
    TTGGTGAGTC TGAGGGAGTC CTACAGGAAC GAGATCATTC CATACAGGAA GCTGACGTGG
    CGGTCGGGGC AGGCTGACAG AGAGACGTGC GCCATGAAGG GAAAACACAG AGACGAGTGC
    CATAATTTCA TTAAAGTCCT GGTTCCGAGA AATGATGATC TCGTGTTCAT CTGCGGAACA
    AATGGTTTCA ATCCCATGTG CCGTTACTAC AGGTTGGATA ACCTGGAATT TGATGGGGAA
    GAGATCAGCG GTTTGGCCCG ATGCCCGTTT GACGCTAAGC AGACAAACGT TGCCCTGTTT
    GCCGATGGTA AGCTGTACTC TGCTACGGTG GCAGACTTCC TGGCTAGCGA TGCTGTGATT
    TATCGCAGTA TGGGAGATGG TTCTGCCCTT CGCACTATCA AGTATGACTC CAAGTGGTTG
    AAAGAGCCAC ATTTTCTTCA CGCTGTGGAC TACGGGGATT TTGTCTACTT CTTCTTTAGA
    GAAATTGCAG CAGAGCACAA CAACTTGGGA AAGGCGGTTT ACTCCAGAGT GGCTCGGATC
    TGTAAAAATG ACATGGGAGG GTCTCAGCGG GTGCTGGAAA AACACTGGAC ATCTTTTGTT
    AAGGCTCGGC TGAACTGTTC CGTTCCTGGA GAATCGTTTT TCTACTTTGA CGTCCTTCAG
    GCCATTACCG ATATCATCGA CATTAATGGG GTGCCTTCTG TGGTTGGAGT TTTCACCACT
    CAGTTAAACA GTATTCCTGG ATCTGCAGTG TGTGCCTTCT CCATGGTGGA CATTGAGAAA
    GCCTTCCGCG GCCGTTTCAA AGAGCAGAAG ACGCCAGATT CAGTGTGGAC GCCATTTCCA
    GAGGAGAAAC TGCCCAAGCC CAGACCTGGC TGCTGTGCAG GACACGGTTC AGCAGAGTCC
    TACAAAACCT CCATCGAGTT TCCAGACGAG ACCCTTCAAT TCATCAAGTC ACACCCACTC
    ATGGACGCCT CGGTCCCCTC CATTGGAGAC GAACCGTGGT TCACCAAAAC AAGAGTCAGG
    TACAGACTAA CAGCACTTGC AGTTGACAAC ACGGCAGGAC CCCATAAGAA TTACACAGTG
    GTTTTCATCG GCTCAGAAGC AGGCATCGTG CTAAAAGTTC TAGCCAAGAC ATCCCCGCTG
    TCTTTGAATG ACAGCGTCCT GCTGGAGGAA ATTGACGTTT TCAACCAGGC CAAGTGTTTA
    TCCAGCAATG AGGACGATCG GCGAATTTTG TCTTTCCACC TAGATAAAGA CAGTCACACA
    CTTTATGTGG CCTTTTCCAG CTGTGTGGTA AGAATACCAC TCAGCCGCTG CGAACGACAC
    AGTTCCTGTC AAAAGTCTTG TATTGCTTCA AGAGACCCAT ACTGCGGATG GATGTCACAT
    GGAGCTTGTG AACGTATTCC TGCAACTATG CAAAGTGGTT ATGAGCAAGA TGTTGAATAT
    GGCAACACTG CTCACCTCGG GGATTGTCAC GACATGGAGT TGCCATCTTC ATCAGTCACA
    GTGCCCGCGA CTTCTGAACC CATACAATCA CCCCAAACCA ACCCCACTCA GACACCACAA
    CTCTTTGAAC CCCATGGTGA CCCAGCCACT TCACATTCTG TTGAGCATAT ACCAGGTGTG
    CTGGAGGGTG TTTGGGAAAT ACAAGCAGGT GAATCAAACC AGATGGTGCA CATGAACATC
    CTGATCTCCT GTGTGTTAGC TGCGTTTCTG CTTGGTGCAT TCATCGCAGG CATGGTTGTT
    TACTGCTATA GAGATGCGTT TCTTCGCACT CCACGAAAGA TCCAAAAAGA TGCAGAGTCG
    GCACAATCCT GCACTGATTC CACTGGCAGC TTTGCCAAGC TTAACGGTCT GTTTGACAGT
    CCTGTCAAAG AGTACCAGCC TAATTTGGAT ACTCCCAAAC TCTACACCAA CCTGTTGAGC
    AATGGAAGGG AGGTGCCAAC CACTGGAGAA ACTAAGACTA TGCTCCTGAG TGGGCAGCCA
    CCGGAATTAG CAGCACTGCC TACGCCTGAA TCCACACCTG TGCTGCAACA GAAGAGCCTC
    CAGCCCATTA AGAACCAGTG GGAGAGAGCT CATGGAAAGA TGAGTGGCTC CCGCAAAGAT
    GCACCACCCA AAAGCCCACA GTACCTTCCC TCTAGTCCAC CTCCCCATTC TGCAATCAAT
    CCACACATAC CTAGTGCTGT GGTACTGCCT AATGCCACGC ATGAAAACAA AGCAGGCTTC
    AGCACACCAG AGGGGCCTCA AGCAGAAAAG AAGGTCCAGA ATAGTGATCA GCATGGTTCC
    AAATCCGGAC GTAAAGACCA GAGGCGAACA GTGGATGCCA GGAATACGTT AAACGACCTC
    TTGAAACATC TAAATGATAG CAACCCCAAG GCCATCATGG TAGAGATGCC CAAATCTCGA
    CAGCACCTAA TGCTGGAACC TATTGTAAGT CCAACAGAGA TTCCACCTAA AGTCCCAAGT
    CGAGAGGCCT CCTTGTATTC TCCGTCTTCC TCGTTACCAA GGAACAGCCC GACCAAGAGA
    GTGGATGTGC CGACAACGCC AACGTCGCCC ACAGGGCATA TGGGAACTCT TGAGAGACAG
    CGGTATCACC GAGGCTCTTC CCAGCGACAC TCCATATCCT CGCCGCCCAA AGGGCTGCAC
    TCACCTAGCG GGGCCATAGT GTCCCGGCAA CCTAGCATGA ACAGAGGTGG GTACATACCC
    CCCACTCCTA CCCCACCCAC TAGACTAGAC TCTCATGGGG TACCAATGGC AATGACGCCT
    TCCGTATCCC GACAGAGCAG CTACAGTGGG CATGGATCTT TACCACGAAC ATCCATTAAA
    AGGACAGCAT CGCTAAAGCC CGATGTGCCA CCCAAACCCA ACGGCTTTGC ACCACAGACT
    GCACAAATGA GGCCAGTGAA CAAGTATAGC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173706.1
    CDS443..3598
    Translation

    Target ORF information:

    RefSeq Version XM_005173649.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D, like (sema6dl), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173649.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGTGGC GAGAACTGAC GATTGACCTG CTGATACTCC TGCTGGTGGC GTCCCGTCTG 
    GATGCTGTGA GCTTTCCTGA GGACAACATC CCTTTAGACG TTGTGGACAG ACACTATGCA
    CGGCAGTACC CGGTGTTTCG TGGACGTCCC TCTGGTAACG AATCTCAGCA CAGACTGGAC
    TTTCAGCTGA TGACCCAGAT ACATGATACG CTGTTCATCG CGGGCAGGGA TCAGGTATAT
    TTGGTGAGTC TGAGGGAGTC CTACAGGAAC GAGATCATTC CATACAGGAA GCTGACGTGG
    CGGTCGGGGC AGGCTGACAG AGAGACGTGC GCCATGAAGG GAAAACACAG AGACGAGTGC
    CATAATTTCA TTAAAGTCCT GGTTCCGAGA AATGATGATC TCGTGTTCAT CTGCGGAACA
    AATGGTTTCA ATCCCATGTG CCGTTACTAC AGGTTGGATA ACCTGGAATT TGATGGGGAA
    GAGATCAGCG GTTTGGCCCG ATGCCCGTTT GACGCTAAGC AGACAAACGT TGCCCTGTTT
    GCCGATGGTA AGCTGTACTC TGCTACGGTG GCAGACTTCC TGGCTAGCGA TGCTGTGATT
    TATCGCAGTA TGGGAGATGG TTCTGCCCTT CGCACTATCA AGTATGACTC CAAGTGGTTG
    AAAGAGCCAC ATTTTCTTCA CGCTGTGGAC TACGGGGATT TTGTCTACTT CTTCTTTAGA
    GAAATTGCAG CAGAGCACAA CAACTTGGGA AAGGCGGTTT ACTCCAGAGT GGCTCGGATC
    TGTAAAAATG ACATGGGAGG GTCTCAGCGG GTGCTGGAAA AACACTGGAC ATCTTTTGTT
    AAGGCTCGGC TGAACTGTTC CGTTCCTGGA GAATCGTTTT TCTACTTTGA CGTCCTTCAG
    GCCATTACCG ATATCATCGA CATTAATGGG GTGCCTTCTG TGGTTGGAGT TTTCACCACT
    CAGTTAAACA GTATTCCTGG ATCTGCAGTG TGTGCCTTCT CCATGGTGGA CATTGAGAAA
    GCCTTCCGCG GCCGTTTCAA AGAGCAGAAG ACGCCAGATT CAGTGTGGAC GCCATTTCCA
    GAGGAGAAAC TGCCCAAGCC CAGACCTGGC TGCTGTGCAG GACACGGTTC AGCAGAGTCC
    TACAAAACCT CCATCGAGTT TCCAGACGAG ACCCTTCAAT TCATCAAGTC ACACCCACTC
    ATGGACGCCT CGGTCCCCTC CATTGGAGAC GAACCGTGGT TCACCAAAAC AAGAGTCAGG
    TACAGACTAA CAGCACTTGC AGTTGACAAC ACGGCAGGAC CCCATAAGAA TTACACAGTG
    GTTTTCATCG GCTCAGAAGC AGGCATCGTG CTAAAAGTTC TAGCCAAGAC ATCCCCGCTG
    TCTTTGAATG ACAGCGTCCT GCTGGAGGAA ATTGACGTTT TCAACCAGGC CAAGTGTTTA
    TCCAGCAATG AGGACGATCG GCGAATTTTG TCTTTCCACC TAGATAAAGA CAGTCACACA
    CTTTATGTGG CCTTTTCCAG CTGTGTGGTA AGAATACCAC TCAGCCGCTG CGAACGACAC
    AGTTCCTGTC AAAAGTCTTG TATTGCTTCA AGAGACCCAT ACTGCGGATG GATGTCACAT
    GGAGCTTGTG AACGTATTCC TGCAACTATG CAAAGTGGTT ATGAGCAAGA TGTTGAATAT
    GGCAACACTG CTCACCTCGG GGATTGTCAC GACATGGAGT TGCCATCTTC ATCAGTCACA
    GTGCCCGCGA CTTCTGAACC CATACAATCA CCCCAAACCA ACCCCACTCA GACACCACAA
    CTCTTTGAAC CCCATGGTGA CCCAGCCACT TCACATTCTG TTGAGCATAT ACCAGGTGTG
    CTGGAGGGTG TTTGGGAAAT ACAAGCAGGT GAATCAAACC AGATGGTGCA CATGAACATC
    CTGATCTCCT GTGTGTTAGC TGCGTTTCTG CTTGGTGCAT TCATCGCAGG CATGGTTGTT
    TACTGCTATA GAGATGCGTT TCTTCGCACT CCACGAAAGA TCCAAAAAGA TGCAGAGTCG
    GCACAATCCT GCACTGATTC CACTGGCAGC TTTGCCAAGC TTAACGGTCT GTTTGACAGT
    CCTGTCAAAG AGTACCAGCC TAATTTGGAT ACTCCCAAAC TCTACACCAA CCTGTTGAGC
    AATGGAAGGG AGGTGCCAAC CACTGGAGAA ACTAAGACTA TGCTCCTGAG TGGGCAGCCA
    CCGGAATTAG CAGCACTGCC TACGCCTGAA TCCACACCTG TGCTGCAACA GAAGAGCCTC
    CAGCCCATTA AGAACCAGTG GGAGAGAGCT CATGGAAAGA TGAGTGGCTC CCGCAAAGAT
    GCACCACCCA AAAGCCCACA GTACCTTCCC TCTAGTCCAC CTCCCCATTC TGCAATCAAT
    CCACACATAC CTAGTGCTGT GGTACTGCCT AATGCCACGC ATGAAAACAA AGCAGGCTTC
    AGCACACCAG AGGGGCCTCA AGCAGAAAAG AAGGTCCAGA ATAGTGATCA GCATGGTTCC
    AAATCCGGAC GTAAAGACCA GAGGCGAACA GTGGATGCCA GGAATACGTT AAACGACCTC
    TTGAAACATC TAAATGATAG CAACCCCAAG GCCATCATGG TAGAGATGCC CAAATCTCGA
    CAGCACCTAA TGCTGGAACC TATTGTAAGT CCAACAGAGA TTCCACCTAA AGTCCCAAGT
    CGAGAGGCCT CCTTGTATTC TCCGTCTTCC TCGTTACCAA GGAACAGCCC GACCAAGAGA
    GTGGATGTGC CGACAACGCC AACGTCGCCC ACAGGGCATA TGGGAACTCT TGAGAGACAG
    CGGTATCACC GAGGCTCTTC CCAGCGACAC TCCATATCCT CGCCGCCCAA AGGGCTGCAC
    TCACCTAGCG GGGCCATAGT GTCCCGGCAA CCTAGCATGA ACAGAGGTGG GTACATACCC
    CCCACTCCTA CCCCACCCAC TAGACTAGAC TCTCATGGGG TACCAATGGC AATGACGCCT
    TCCGTATCCC GACAGAGCAG CTACAGTGGG CATGGATCTT TACCACGAAC ATCCATTAAA
    AGGACAGCAT CGCTAAAGCC CGATGTGCCA CCCAAACCCA ACGGCTTTGC ACCACAGACT
    GCACAAATGA GGCCAGTGAA CAAGTATAGC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23485
    Clone ID Related Accession (Same CDS sequence) XM_005173649.3 , XM_005173649.4
    Accession Version XM_005173649.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3156bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product semaphorin-6D isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173649.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGTGGC GAGAACTGAC GATTGACCTG CTGATACTCC TGCTGGTGGC GTCCCGTCTG 
    GATGCTGTGA GCTTTCCTGA GGACAACATC CCTTTAGACG TTGTGGACAG ACACTATGCA
    CGGCAGTACC CGGTGTTTCG TGGACGTCCC TCTGGTAACG AATCTCAGCA CAGACTGGAC
    TTTCAGCTGA TGACCCAGAT ACATGATACG CTGTTCATCG CGGGCAGGGA TCAGGTATAT
    TTGGTGAGTC TGAGGGAGTC CTACAGGAAC GAGATCATTC CATACAGGAA GCTGACGTGG
    CGGTCGGGGC AGGCTGACAG AGAGACGTGC GCCATGAAGG GAAAACACAG AGACGAGTGC
    CATAATTTCA TTAAAGTCCT GGTTCCGAGA AATGATGATC TCGTGTTCAT CTGCGGAACA
    AATGGTTTCA ATCCCATGTG CCGTTACTAC AGGTTGGATA ACCTGGAATT TGATGGGGAA
    GAGATCAGCG GTTTGGCCCG ATGCCCGTTT GACGCTAAGC AGACAAACGT TGCCCTGTTT
    GCCGATGGTA AGCTGTACTC TGCTACGGTG GCAGACTTCC TGGCTAGCGA TGCTGTGATT
    TATCGCAGTA TGGGAGATGG TTCTGCCCTT CGCACTATCA AGTATGACTC CAAGTGGTTG
    AAAGAGCCAC ATTTTCTTCA CGCTGTGGAC TACGGGGATT TTGTCTACTT CTTCTTTAGA
    GAAATTGCAG CAGAGCACAA CAACTTGGGA AAGGCGGTTT ACTCCAGAGT GGCTCGGATC
    TGTAAAAATG ACATGGGAGG GTCTCAGCGG GTGCTGGAAA AACACTGGAC ATCTTTTGTT
    AAGGCTCGGC TGAACTGTTC CGTTCCTGGA GAATCGTTTT TCTACTTTGA CGTCCTTCAG
    GCCATTACCG ATATCATCGA CATTAATGGG GTGCCTTCTG TGGTTGGAGT TTTCACCACT
    CAGTTAAACA GTATTCCTGG ATCTGCAGTG TGTGCCTTCT CCATGGTGGA CATTGAGAAA
    GCCTTCCGCG GCCGTTTCAA AGAGCAGAAG ACGCCAGATT CAGTGTGGAC GCCATTTCCA
    GAGGAGAAAC TGCCCAAGCC CAGACCTGGC TGCTGTGCAG GACACGGTTC AGCAGAGTCC
    TACAAAACCT CCATCGAGTT TCCAGACGAG ACCCTTCAAT TCATCAAGTC ACACCCACTC
    ATGGACGCCT CGGTCCCCTC CATTGGAGAC GAACCGTGGT TCACCAAAAC AAGAGTCAGG
    TACAGACTAA CAGCACTTGC AGTTGACAAC ACGGCAGGAC CCCATAAGAA TTACACAGTG
    GTTTTCATCG GCTCAGAAGC AGGCATCGTG CTAAAAGTTC TAGCCAAGAC ATCCCCGCTG
    TCTTTGAATG ACAGCGTCCT GCTGGAGGAA ATTGACGTTT TCAACCAGGC CAAGTGTTTA
    TCCAGCAATG AGGACGATCG GCGAATTTTG TCTTTCCACC TAGATAAAGA CAGTCACACA
    CTTTATGTGG CCTTTTCCAG CTGTGTGGTA AGAATACCAC TCAGCCGCTG CGAACGACAC
    AGTTCCTGTC AAAAGTCTTG TATTGCTTCA AGAGACCCAT ACTGCGGATG GATGTCACAT
    GGAGCTTGTG AACGTATTCC TGCAACTATG CAAAGTGGTT ATGAGCAAGA TGTTGAATAT
    GGCAACACTG CTCACCTCGG GGATTGTCAC GACATGGAGT TGCCATCTTC ATCAGTCACA
    GTGCCCGCGA CTTCTGAACC CATACAATCA CCCCAAACCA ACCCCACTCA GACACCACAA
    CTCTTTGAAC CCCATGGTGA CCCAGCCACT TCACATTCTG TTGAGCATAT ACCAGGTGTG
    CTGGAGGGTG TTTGGGAAAT ACAAGCAGGT GAATCAAACC AGATGGTGCA CATGAACATC
    CTGATCTCCT GTGTGTTAGC TGCGTTTCTG CTTGGTGCAT TCATCGCAGG CATGGTTGTT
    TACTGCTATA GAGATGCGTT TCTTCGCACT CCACGAAAGA TCCAAAAAGA TGCAGAGTCG
    GCACAATCCT GCACTGATTC CACTGGCAGC TTTGCCAAGC TTAACGGTCT GTTTGACAGT
    CCTGTCAAAG AGTACCAGCC TAATTTGGAT ACTCCCAAAC TCTACACCAA CCTGTTGAGC
    AATGGAAGGG AGGTGCCAAC CACTGGAGAA ACTAAGACTA TGCTCCTGAG TGGGCAGCCA
    CCGGAATTAG CAGCACTGCC TACGCCTGAA TCCACACCTG TGCTGCAACA GAAGAGCCTC
    CAGCCCATTA AGAACCAGTG GGAGAGAGCT CATGGAAAGA TGAGTGGCTC CCGCAAAGAT
    GCACCACCCA AAAGCCCACA GTACCTTCCC TCTAGTCCAC CTCCCCATTC TGCAATCAAT
    CCACACATAC CTAGTGCTGT GGTACTGCCT AATGCCACGC ATGAAAACAA AGCAGGCTTC
    AGCACACCAG AGGGGCCTCA AGCAGAAAAG AAGGTCCAGA ATAGTGATCA GCATGGTTCC
    AAATCCGGAC GTAAAGACCA GAGGCGAACA GTGGATGCCA GGAATACGTT AAACGACCTC
    TTGAAACATC TAAATGATAG CAACCCCAAG GCCATCATGG TAGAGATGCC CAAATCTCGA
    CAGCACCTAA TGCTGGAACC TATTGTAAGT CCAACAGAGA TTCCACCTAA AGTCCCAAGT
    CGAGAGGCCT CCTTGTATTC TCCGTCTTCC TCGTTACCAA GGAACAGCCC GACCAAGAGA
    GTGGATGTGC CGACAACGCC AACGTCGCCC ACAGGGCATA TGGGAACTCT TGAGAGACAG
    CGGTATCACC GAGGCTCTTC CCAGCGACAC TCCATATCCT CGCCGCCCAA AGGGCTGCAC
    TCACCTAGCG GGGCCATAGT GTCCCGGCAA CCTAGCATGA ACAGAGGTGG GTACATACCC
    CCCACTCCTA CCCCACCCAC TAGACTAGAC TCTCATGGGG TACCAATGGC AATGACGCCT
    TCCGTATCCC GACAGAGCAG CTACAGTGGG CATGGATCTT TACCACGAAC ATCCATTAAA
    AGGACAGCAT CGCTAAAGCC CGATGTGCCA CCCAAACCCA ACGGCTTTGC ACCACAGACT
    GCACAAATGA GGCCAGTGAA CAAGTATAGC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173706.1
    CDS219..3374
    Translation

    Target ORF information:

    RefSeq Version XM_005173649.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D, like (sema6dl), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173649.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGTGGC GAGAACTGAC GATTGACCTG CTGATACTCC TGCTGGTGGC GTCCCGTCTG 
    GATGCTGTGA GCTTTCCTGA GGACAACATC CCTTTAGACG TTGTGGACAG ACACTATGCA
    CGGCAGTACC CGGTGTTTCG TGGACGTCCC TCTGGTAACG AATCTCAGCA CAGACTGGAC
    TTTCAGCTGA TGACCCAGAT ACATGATACG CTGTTCATCG CGGGCAGGGA TCAGGTATAT
    TTGGTGAGTC TGAGGGAGTC CTACAGGAAC GAGATCATTC CATACAGGAA GCTGACGTGG
    CGGTCGGGGC AGGCTGACAG AGAGACGTGC GCCATGAAGG GAAAACACAG AGACGAGTGC
    CATAATTTCA TTAAAGTCCT GGTTCCGAGA AATGATGATC TCGTGTTCAT CTGCGGAACA
    AATGGTTTCA ATCCCATGTG CCGTTACTAC AGGTTGGATA ACCTGGAATT TGATGGGGAA
    GAGATCAGCG GTTTGGCCCG ATGCCCGTTT GACGCTAAGC AGACAAACGT TGCCCTGTTT
    GCCGATGGTA AGCTGTACTC TGCTACGGTG GCAGACTTCC TGGCTAGCGA TGCTGTGATT
    TATCGCAGTA TGGGAGATGG TTCTGCCCTT CGCACTATCA AGTATGACTC CAAGTGGTTG
    AAAGAGCCAC ATTTTCTTCA CGCTGTGGAC TACGGGGATT TTGTCTACTT CTTCTTTAGA
    GAAATTGCAG CAGAGCACAA CAACTTGGGA AAGGCGGTTT ACTCCAGAGT GGCTCGGATC
    TGTAAAAATG ACATGGGAGG GTCTCAGCGG GTGCTGGAAA AACACTGGAC ATCTTTTGTT
    AAGGCTCGGC TGAACTGTTC CGTTCCTGGA GAATCGTTTT TCTACTTTGA CGTCCTTCAG
    GCCATTACCG ATATCATCGA CATTAATGGG GTGCCTTCTG TGGTTGGAGT TTTCACCACT
    CAGTTAAACA GTATTCCTGG ATCTGCAGTG TGTGCCTTCT CCATGGTGGA CATTGAGAAA
    GCCTTCCGCG GCCGTTTCAA AGAGCAGAAG ACGCCAGATT CAGTGTGGAC GCCATTTCCA
    GAGGAGAAAC TGCCCAAGCC CAGACCTGGC TGCTGTGCAG GACACGGTTC AGCAGAGTCC
    TACAAAACCT CCATCGAGTT TCCAGACGAG ACCCTTCAAT TCATCAAGTC ACACCCACTC
    ATGGACGCCT CGGTCCCCTC CATTGGAGAC GAACCGTGGT TCACCAAAAC AAGAGTCAGG
    TACAGACTAA CAGCACTTGC AGTTGACAAC ACGGCAGGAC CCCATAAGAA TTACACAGTG
    GTTTTCATCG GCTCAGAAGC AGGCATCGTG CTAAAAGTTC TAGCCAAGAC ATCCCCGCTG
    TCTTTGAATG ACAGCGTCCT GCTGGAGGAA ATTGACGTTT TCAACCAGGC CAAGTGTTTA
    TCCAGCAATG AGGACGATCG GCGAATTTTG TCTTTCCACC TAGATAAAGA CAGTCACACA
    CTTTATGTGG CCTTTTCCAG CTGTGTGGTA AGAATACCAC TCAGCCGCTG CGAACGACAC
    AGTTCCTGTC AAAAGTCTTG TATTGCTTCA AGAGACCCAT ACTGCGGATG GATGTCACAT
    GGAGCTTGTG AACGTATTCC TGCAACTATG CAAAGTGGTT ATGAGCAAGA TGTTGAATAT
    GGCAACACTG CTCACCTCGG GGATTGTCAC GACATGGAGT TGCCATCTTC ATCAGTCACA
    GTGCCCGCGA CTTCTGAACC CATACAATCA CCCCAAACCA ACCCCACTCA GACACCACAA
    CTCTTTGAAC CCCATGGTGA CCCAGCCACT TCACATTCTG TTGAGCATAT ACCAGGTGTG
    CTGGAGGGTG TTTGGGAAAT ACAAGCAGGT GAATCAAACC AGATGGTGCA CATGAACATC
    CTGATCTCCT GTGTGTTAGC TGCGTTTCTG CTTGGTGCAT TCATCGCAGG CATGGTTGTT
    TACTGCTATA GAGATGCGTT TCTTCGCACT CCACGAAAGA TCCAAAAAGA TGCAGAGTCG
    GCACAATCCT GCACTGATTC CACTGGCAGC TTTGCCAAGC TTAACGGTCT GTTTGACAGT
    CCTGTCAAAG AGTACCAGCC TAATTTGGAT ACTCCCAAAC TCTACACCAA CCTGTTGAGC
    AATGGAAGGG AGGTGCCAAC CACTGGAGAA ACTAAGACTA TGCTCCTGAG TGGGCAGCCA
    CCGGAATTAG CAGCACTGCC TACGCCTGAA TCCACACCTG TGCTGCAACA GAAGAGCCTC
    CAGCCCATTA AGAACCAGTG GGAGAGAGCT CATGGAAAGA TGAGTGGCTC CCGCAAAGAT
    GCACCACCCA AAAGCCCACA GTACCTTCCC TCTAGTCCAC CTCCCCATTC TGCAATCAAT
    CCACACATAC CTAGTGCTGT GGTACTGCCT AATGCCACGC ATGAAAACAA AGCAGGCTTC
    AGCACACCAG AGGGGCCTCA AGCAGAAAAG AAGGTCCAGA ATAGTGATCA GCATGGTTCC
    AAATCCGGAC GTAAAGACCA GAGGCGAACA GTGGATGCCA GGAATACGTT AAACGACCTC
    TTGAAACATC TAAATGATAG CAACCCCAAG GCCATCATGG TAGAGATGCC CAAATCTCGA
    CAGCACCTAA TGCTGGAACC TATTGTAAGT CCAACAGAGA TTCCACCTAA AGTCCCAAGT
    CGAGAGGCCT CCTTGTATTC TCCGTCTTCC TCGTTACCAA GGAACAGCCC GACCAAGAGA
    GTGGATGTGC CGACAACGCC AACGTCGCCC ACAGGGCATA TGGGAACTCT TGAGAGACAG
    CGGTATCACC GAGGCTCTTC CCAGCGACAC TCCATATCCT CGCCGCCCAA AGGGCTGCAC
    TCACCTAGCG GGGCCATAGT GTCCCGGCAA CCTAGCATGA ACAGAGGTGG GTACATACCC
    CCCACTCCTA CCCCACCCAC TAGACTAGAC TCTCATGGGG TACCAATGGC AATGACGCCT
    TCCGTATCCC GACAGAGCAG CTACAGTGGG CATGGATCTT TACCACGAAC ATCCATTAAA
    AGGACAGCAT CGCTAAAGCC CGATGTGCCA CCCAAACCCA ACGGCTTTGC ACCACAGACT
    GCACAAATGA GGCCAGTGAA CAAGTATAGC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41756
    Clone ID Related Accession (Same CDS sequence) XM_009293807.3 , XM_009293807.2
    Accession Version XM_009293807.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3201bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product semaphorin-6D isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293807.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTGGC GAGAACTGAC GATTGACCTG CTGATACTCC TGCTGGTGGC GTCCCGTCTG 
    GATGCTGTGA GCTTTCCTGA GGACAACATC CCTTTAGACG TTGTGGACAG ACACTATGCA
    CGGCAGTACC CGGTGTTTCG TGGACGTCCC TCTGGTAACG AATCTCAGCA CAGACTGGAC
    TTTCAGCTGA TGACCCAGAT ACATGATACG CTGTTCATCG CGGGCAGGGA TCAGGTATAT
    TTGGTGAGTC TGAGGGAGTC CTACAGGAAC GAGATCATTC CATACAGGAA GCTGACGTGG
    CGGTCGGGGC AGGCTGACAG AGAGACGTGC GCCATGAAGG GAAAACACAG AGACGAGTGC
    CATAATTTCA TTAAAGTCCT GGTTCCGAGA AATGATGATC TCGTGTTCAT CTGCGGAACA
    AATGGTTTCA ATCCCATGTG CCGTTACTAC AGGTTGGATA ACCTGGAATT TGATGGGGAA
    GAGATCAGCG GTTTGGCCCG ATGCCCGTTT GACGCTAAGC AGACAAACGT TGCCCTGTTT
    GCCGATGGTA AGCTGTACTC TGCTACGGTG GCAGACTTCC TGGCTAGCGA TGCTGTGATT
    TATCGCAGTA TGGGAGATGG TTCTGCCCTT CGCACTATCA AGTATGACTC CAAGTGGTTG
    AAAGAGCCAC ATTTTCTTCA CGCTGTGGAC TACGGGGATT TTGTCTACTT CTTCTTTAGA
    GAAATTGCAG CAGAGCACAA CAACTTGGGA AAGGCGGTTT ACTCCAGAGT GGCTCGGATC
    TGTAAAAATG ACATGGGAGG GTCTCAGCGG GTGCTGGAAA AACACTGGAC ATCTTTTGTT
    AAGGCTCGGC TGAACTGTTC CGTTCCTGGA GAATCGTTTT TCTACTTTGA CGTCCTTCAG
    GCCATTACCG ATATCATCGA CATTAATGGG GTGCCTTCTG TGGTTGGAGT TTTCACCACT
    CAGTTAAACA GTATTCCTGG ATCTGCAGTG TGTGCCTTCT CCATGGTGGA CATTGAGAAA
    GCCTTCCGCG GCCGTTTCAA AGAGCAGAAG ACGCCAGATT CAGTGTGGAC GCCATTTCCA
    GAGGAGAAAC TGCCCAAGCC CAGACCTGGC TGCTGTGCAG GACACGGTTC AGCAGAGTCC
    TACAAAACCT CCATCGAGTT TCCAGACGAG ACCCTTCAAT TCATCAAGTC ACACCCACTC
    ATGGACGCCT CGGTCCCCTC CATTGGAGAC GAACCGTGGT TCACCAAAAC AAGAGTCAGG
    TACAGACTAA CAGCACTTGC AGTTGACAAC ACGGCAGGAC CCCATAAGAA TTACACAGTG
    GTTTTCATCG GCTCAGAAGC AGGCATCGTG CTAAAAGTTC TAGCCAAGAC ATCCCCGCTG
    TCTTTGAATG ACAGCGTCCT GCTGGAGGAA ATTGACGTTT TCAACCAGGC CAAGTGTTTA
    TCCAGCAATG AGGACGATCG GCGAATTTTG TCTTTCCACC TAGATAAAGA CAGTCACACA
    CTTTATGTGG CCTTTTCCAG CTGTGTGGTA AGAATACCAC TCAGCCGCTG CGAACGACAC
    AGTTCCTGTC AAAAGTCTTG TATTGCTTCA AGAGACCCAT ACTGCGGATG GATGTCACAT
    GGAGCTTGTG AACGTATTCC TGCAACTATG CAAAGTGGTT ATGAGCAAGA TGTTGAATAT
    GGCAACACTG CTCACCTCGG GGATTGTCAC GAATTTTTGG CCACTACATC AGCGCCAGAT
    TTCAAATCAT ATGGCGACCC AACCTCTGAC ATGGAGTTGC CATCTTCATC AGTCACAGTG
    CCCGCGACTT CTGAACCCAT ACAATCACCC CAAACCAACC CCACTCAGAC ACCACAACTC
    TTTGAACCCC ATGGTGACCC AGCCACTTCA CATTCTGTTG AGCATATACC AGGTGTTTGG
    GAAATACAAG CAGGTGAATC AAACCAGATG GTGCACATGA ACATCCTGAT CTCCTGTGTG
    TTAGCTGCGT TTCTGCTTGG TGCATTCATC GCAGGCATGG TTGTTTACTG CTATAGAGAT
    GCGTTTCTTC GCACTCCACG AAAGATCCAA AAAGATGCAG AGTCGGCACA ATCCTGCACT
    GATTCCACTG GCAGCTTTGC CAAGCTTAAC GGTCTGTTTG ACAGTCCTGT CAAAGAGTAC
    CAGCCTAATT TGGATACTCC CAAACTCTAC ACCAACCTGT TGAGCAATGG AAGGGAGGTG
    CCAACCACTG GAGAAACTAA GACTATGCTC CTGAGTGGGC AGCCACCGGA ATTAGCAGCA
    CTGCCTACGC CTGAATCCAC ACCTGTGCTG CAACAGAAGA GCCTCCAGCC CATTAAGAAC
    CAGTGGGAGA GAGCTCATGG AAAGATGAGT GGCTCCCGCA AAGATGCACC ACCCAAAAGC
    CCACAGTACC TTCCCTCTAG TCCACCTCCC CATTCTGCAA TCAATCCACA CATACCTAGT
    GCTGTGGTAC TGCCTAATGC CACGCATGAA AACAAAGCAG GCTTCAGCAC ACCAGAGGGG
    CCTCAAGCAG AAAAGAAGGT CCAGAATAGT GATCAGCATG GTTCCAAATC CGGACGTAAA
    GACCAGAGGC GAACAGTGGA TGCCAGGAAT ACGTTAAACG ACCTCTTGAA ACATCTAAAT
    GATAGCAACC CCAAGGCCAT CATGGTAGAG ATGCCCAAAT CTCGACAGCA CCTAATGCTG
    GAACCTATTG TAAGTCCAAC AGAGATTCCA CCTAAAGTCC CAAGTCGAGA GGCCTCCTTG
    TATTCTCCGT CTTCCTCGTT ACCAAGGAAC AGCCCGACCA AGAGAGTGGA TGTGCCGACA
    ACGCCAACGT CGCCCACAGG GCATATGGGA ACTCTTGAGA GACAGCGGTA TCACCGAGGC
    TCTTCCCAGC GACACTCCAT ATCCTCGCCG CCCAAAGGGC TGCACTCACC TAGCGGGGCC
    ATAGTGTCCC GGCAACCTAG CATGAACAGA GGTGGGTACA TACCCCCCAC TCCTACCCCA
    CCCACTAGAC TAGACTCTCA TGGGGTACCA ATGGCAATGA CGCCTTCCGT ATCCCGACAG
    AGCAGCTACA GTGGGCATGG ATCTTTACCA CGAACATCCA TTAAAAGGAC AGCATCGCTA
    AAGCCCGATG TGCCACCCAA ACCCAACGGC TTTGCACCAC AGACTGCACA AATGAGGCCA
    GTGAACAAGT ATAGCTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292082.1
    CDS219..3419
    Translation

    Target ORF information:

    RefSeq Version XM_009293807.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D, like (sema6dl), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293807.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTGGC GAGAACTGAC GATTGACCTG CTGATACTCC TGCTGGTGGC GTCCCGTCTG 
    GATGCTGTGA GCTTTCCTGA GGACAACATC CCTTTAGACG TTGTGGACAG ACACTATGCA
    CGGCAGTACC CGGTGTTTCG TGGACGTCCC TCTGGTAACG AATCTCAGCA CAGACTGGAC
    TTTCAGCTGA TGACCCAGAT ACATGATACG CTGTTCATCG CGGGCAGGGA TCAGGTATAT
    TTGGTGAGTC TGAGGGAGTC CTACAGGAAC GAGATCATTC CATACAGGAA GCTGACGTGG
    CGGTCGGGGC AGGCTGACAG AGAGACGTGC GCCATGAAGG GAAAACACAG AGACGAGTGC
    CATAATTTCA TTAAAGTCCT GGTTCCGAGA AATGATGATC TCGTGTTCAT CTGCGGAACA
    AATGGTTTCA ATCCCATGTG CCGTTACTAC AGGTTGGATA ACCTGGAATT TGATGGGGAA
    GAGATCAGCG GTTTGGCCCG ATGCCCGTTT GACGCTAAGC AGACAAACGT TGCCCTGTTT
    GCCGATGGTA AGCTGTACTC TGCTACGGTG GCAGACTTCC TGGCTAGCGA TGCTGTGATT
    TATCGCAGTA TGGGAGATGG TTCTGCCCTT CGCACTATCA AGTATGACTC CAAGTGGTTG
    AAAGAGCCAC ATTTTCTTCA CGCTGTGGAC TACGGGGATT TTGTCTACTT CTTCTTTAGA
    GAAATTGCAG CAGAGCACAA CAACTTGGGA AAGGCGGTTT ACTCCAGAGT GGCTCGGATC
    TGTAAAAATG ACATGGGAGG GTCTCAGCGG GTGCTGGAAA AACACTGGAC ATCTTTTGTT
    AAGGCTCGGC TGAACTGTTC CGTTCCTGGA GAATCGTTTT TCTACTTTGA CGTCCTTCAG
    GCCATTACCG ATATCATCGA CATTAATGGG GTGCCTTCTG TGGTTGGAGT TTTCACCACT
    CAGTTAAACA GTATTCCTGG ATCTGCAGTG TGTGCCTTCT CCATGGTGGA CATTGAGAAA
    GCCTTCCGCG GCCGTTTCAA AGAGCAGAAG ACGCCAGATT CAGTGTGGAC GCCATTTCCA
    GAGGAGAAAC TGCCCAAGCC CAGACCTGGC TGCTGTGCAG GACACGGTTC AGCAGAGTCC
    TACAAAACCT CCATCGAGTT TCCAGACGAG ACCCTTCAAT TCATCAAGTC ACACCCACTC
    ATGGACGCCT CGGTCCCCTC CATTGGAGAC GAACCGTGGT TCACCAAAAC AAGAGTCAGG
    TACAGACTAA CAGCACTTGC AGTTGACAAC ACGGCAGGAC CCCATAAGAA TTACACAGTG
    GTTTTCATCG GCTCAGAAGC AGGCATCGTG CTAAAAGTTC TAGCCAAGAC ATCCCCGCTG
    TCTTTGAATG ACAGCGTCCT GCTGGAGGAA ATTGACGTTT TCAACCAGGC CAAGTGTTTA
    TCCAGCAATG AGGACGATCG GCGAATTTTG TCTTTCCACC TAGATAAAGA CAGTCACACA
    CTTTATGTGG CCTTTTCCAG CTGTGTGGTA AGAATACCAC TCAGCCGCTG CGAACGACAC
    AGTTCCTGTC AAAAGTCTTG TATTGCTTCA AGAGACCCAT ACTGCGGATG GATGTCACAT
    GGAGCTTGTG AACGTATTCC TGCAACTATG CAAAGTGGTT ATGAGCAAGA TGTTGAATAT
    GGCAACACTG CTCACCTCGG GGATTGTCAC GAATTTTTGG CCACTACATC AGCGCCAGAT
    TTCAAATCAT ATGGCGACCC AACCTCTGAC ATGGAGTTGC CATCTTCATC AGTCACAGTG
    CCCGCGACTT CTGAACCCAT ACAATCACCC CAAACCAACC CCACTCAGAC ACCACAACTC
    TTTGAACCCC ATGGTGACCC AGCCACTTCA CATTCTGTTG AGCATATACC AGGTGTTTGG
    GAAATACAAG CAGGTGAATC AAACCAGATG GTGCACATGA ACATCCTGAT CTCCTGTGTG
    TTAGCTGCGT TTCTGCTTGG TGCATTCATC GCAGGCATGG TTGTTTACTG CTATAGAGAT
    GCGTTTCTTC GCACTCCACG AAAGATCCAA AAAGATGCAG AGTCGGCACA ATCCTGCACT
    GATTCCACTG GCAGCTTTGC CAAGCTTAAC GGTCTGTTTG ACAGTCCTGT CAAAGAGTAC
    CAGCCTAATT TGGATACTCC CAAACTCTAC ACCAACCTGT TGAGCAATGG AAGGGAGGTG
    CCAACCACTG GAGAAACTAA GACTATGCTC CTGAGTGGGC AGCCACCGGA ATTAGCAGCA
    CTGCCTACGC CTGAATCCAC ACCTGTGCTG CAACAGAAGA GCCTCCAGCC CATTAAGAAC
    CAGTGGGAGA GAGCTCATGG AAAGATGAGT GGCTCCCGCA AAGATGCACC ACCCAAAAGC
    CCACAGTACC TTCCCTCTAG TCCACCTCCC CATTCTGCAA TCAATCCACA CATACCTAGT
    GCTGTGGTAC TGCCTAATGC CACGCATGAA AACAAAGCAG GCTTCAGCAC ACCAGAGGGG
    CCTCAAGCAG AAAAGAAGGT CCAGAATAGT GATCAGCATG GTTCCAAATC CGGACGTAAA
    GACCAGAGGC GAACAGTGGA TGCCAGGAAT ACGTTAAACG ACCTCTTGAA ACATCTAAAT
    GATAGCAACC CCAAGGCCAT CATGGTAGAG ATGCCCAAAT CTCGACAGCA CCTAATGCTG
    GAACCTATTG TAAGTCCAAC AGAGATTCCA CCTAAAGTCC CAAGTCGAGA GGCCTCCTTG
    TATTCTCCGT CTTCCTCGTT ACCAAGGAAC AGCCCGACCA AGAGAGTGGA TGTGCCGACA
    ACGCCAACGT CGCCCACAGG GCATATGGGA ACTCTTGAGA GACAGCGGTA TCACCGAGGC
    TCTTCCCAGC GACACTCCAT ATCCTCGCCG CCCAAAGGGC TGCACTCACC TAGCGGGGCC
    ATAGTGTCCC GGCAACCTAG CATGAACAGA GGTGGGTACA TACCCCCCAC TCCTACCCCA
    CCCACTAGAC TAGACTCTCA TGGGGTACCA ATGGCAATGA CGCCTTCCGT ATCCCGACAG
    AGCAGCTACA GTGGGCATGG ATCTTTACCA CGAACATCCA TTAAAAGGAC AGCATCGCTA
    AAGCCCGATG TGCCACCCAA ACCCAACGGC TTTGCACCAC AGACTGCACA AATGAGGCCA
    GTGAACAAGT ATAGCTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41756
    Clone ID Related Accession (Same CDS sequence) XM_009293807.3 , XM_009293807.2
    Accession Version XM_009293807.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3201bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product semaphorin-6D isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293807.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTGGC GAGAACTGAC GATTGACCTG CTGATACTCC TGCTGGTGGC GTCCCGTCTG 
    GATGCTGTGA GCTTTCCTGA GGACAACATC CCTTTAGACG TTGTGGACAG ACACTATGCA
    CGGCAGTACC CGGTGTTTCG TGGACGTCCC TCTGGTAACG AATCTCAGCA CAGACTGGAC
    TTTCAGCTGA TGACCCAGAT ACATGATACG CTGTTCATCG CGGGCAGGGA TCAGGTATAT
    TTGGTGAGTC TGAGGGAGTC CTACAGGAAC GAGATCATTC CATACAGGAA GCTGACGTGG
    CGGTCGGGGC AGGCTGACAG AGAGACGTGC GCCATGAAGG GAAAACACAG AGACGAGTGC
    CATAATTTCA TTAAAGTCCT GGTTCCGAGA AATGATGATC TCGTGTTCAT CTGCGGAACA
    AATGGTTTCA ATCCCATGTG CCGTTACTAC AGGTTGGATA ACCTGGAATT TGATGGGGAA
    GAGATCAGCG GTTTGGCCCG ATGCCCGTTT GACGCTAAGC AGACAAACGT TGCCCTGTTT
    GCCGATGGTA AGCTGTACTC TGCTACGGTG GCAGACTTCC TGGCTAGCGA TGCTGTGATT
    TATCGCAGTA TGGGAGATGG TTCTGCCCTT CGCACTATCA AGTATGACTC CAAGTGGTTG
    AAAGAGCCAC ATTTTCTTCA CGCTGTGGAC TACGGGGATT TTGTCTACTT CTTCTTTAGA
    GAAATTGCAG CAGAGCACAA CAACTTGGGA AAGGCGGTTT ACTCCAGAGT GGCTCGGATC
    TGTAAAAATG ACATGGGAGG GTCTCAGCGG GTGCTGGAAA AACACTGGAC ATCTTTTGTT
    AAGGCTCGGC TGAACTGTTC CGTTCCTGGA GAATCGTTTT TCTACTTTGA CGTCCTTCAG
    GCCATTACCG ATATCATCGA CATTAATGGG GTGCCTTCTG TGGTTGGAGT TTTCACCACT
    CAGTTAAACA GTATTCCTGG ATCTGCAGTG TGTGCCTTCT CCATGGTGGA CATTGAGAAA
    GCCTTCCGCG GCCGTTTCAA AGAGCAGAAG ACGCCAGATT CAGTGTGGAC GCCATTTCCA
    GAGGAGAAAC TGCCCAAGCC CAGACCTGGC TGCTGTGCAG GACACGGTTC AGCAGAGTCC
    TACAAAACCT CCATCGAGTT TCCAGACGAG ACCCTTCAAT TCATCAAGTC ACACCCACTC
    ATGGACGCCT CGGTCCCCTC CATTGGAGAC GAACCGTGGT TCACCAAAAC AAGAGTCAGG
    TACAGACTAA CAGCACTTGC AGTTGACAAC ACGGCAGGAC CCCATAAGAA TTACACAGTG
    GTTTTCATCG GCTCAGAAGC AGGCATCGTG CTAAAAGTTC TAGCCAAGAC ATCCCCGCTG
    TCTTTGAATG ACAGCGTCCT GCTGGAGGAA ATTGACGTTT TCAACCAGGC CAAGTGTTTA
    TCCAGCAATG AGGACGATCG GCGAATTTTG TCTTTCCACC TAGATAAAGA CAGTCACACA
    CTTTATGTGG CCTTTTCCAG CTGTGTGGTA AGAATACCAC TCAGCCGCTG CGAACGACAC
    AGTTCCTGTC AAAAGTCTTG TATTGCTTCA AGAGACCCAT ACTGCGGATG GATGTCACAT
    GGAGCTTGTG AACGTATTCC TGCAACTATG CAAAGTGGTT ATGAGCAAGA TGTTGAATAT
    GGCAACACTG CTCACCTCGG GGATTGTCAC GAATTTTTGG CCACTACATC AGCGCCAGAT
    TTCAAATCAT ATGGCGACCC AACCTCTGAC ATGGAGTTGC CATCTTCATC AGTCACAGTG
    CCCGCGACTT CTGAACCCAT ACAATCACCC CAAACCAACC CCACTCAGAC ACCACAACTC
    TTTGAACCCC ATGGTGACCC AGCCACTTCA CATTCTGTTG AGCATATACC AGGTGTTTGG
    GAAATACAAG CAGGTGAATC AAACCAGATG GTGCACATGA ACATCCTGAT CTCCTGTGTG
    TTAGCTGCGT TTCTGCTTGG TGCATTCATC GCAGGCATGG TTGTTTACTG CTATAGAGAT
    GCGTTTCTTC GCACTCCACG AAAGATCCAA AAAGATGCAG AGTCGGCACA ATCCTGCACT
    GATTCCACTG GCAGCTTTGC CAAGCTTAAC GGTCTGTTTG ACAGTCCTGT CAAAGAGTAC
    CAGCCTAATT TGGATACTCC CAAACTCTAC ACCAACCTGT TGAGCAATGG AAGGGAGGTG
    CCAACCACTG GAGAAACTAA GACTATGCTC CTGAGTGGGC AGCCACCGGA ATTAGCAGCA
    CTGCCTACGC CTGAATCCAC ACCTGTGCTG CAACAGAAGA GCCTCCAGCC CATTAAGAAC
    CAGTGGGAGA GAGCTCATGG AAAGATGAGT GGCTCCCGCA AAGATGCACC ACCCAAAAGC
    CCACAGTACC TTCCCTCTAG TCCACCTCCC CATTCTGCAA TCAATCCACA CATACCTAGT
    GCTGTGGTAC TGCCTAATGC CACGCATGAA AACAAAGCAG GCTTCAGCAC ACCAGAGGGG
    CCTCAAGCAG AAAAGAAGGT CCAGAATAGT GATCAGCATG GTTCCAAATC CGGACGTAAA
    GACCAGAGGC GAACAGTGGA TGCCAGGAAT ACGTTAAACG ACCTCTTGAA ACATCTAAAT
    GATAGCAACC CCAAGGCCAT CATGGTAGAG ATGCCCAAAT CTCGACAGCA CCTAATGCTG
    GAACCTATTG TAAGTCCAAC AGAGATTCCA CCTAAAGTCC CAAGTCGAGA GGCCTCCTTG
    TATTCTCCGT CTTCCTCGTT ACCAAGGAAC AGCCCGACCA AGAGAGTGGA TGTGCCGACA
    ACGCCAACGT CGCCCACAGG GCATATGGGA ACTCTTGAGA GACAGCGGTA TCACCGAGGC
    TCTTCCCAGC GACACTCCAT ATCCTCGCCG CCCAAAGGGC TGCACTCACC TAGCGGGGCC
    ATAGTGTCCC GGCAACCTAG CATGAACAGA GGTGGGTACA TACCCCCCAC TCCTACCCCA
    CCCACTAGAC TAGACTCTCA TGGGGTACCA ATGGCAATGA CGCCTTCCGT ATCCCGACAG
    AGCAGCTACA GTGGGCATGG ATCTTTACCA CGAACATCCA TTAAAAGGAC AGCATCGCTA
    AAGCCCGATG TGCCACCCAA ACCCAACGGC TTTGCACCAC AGACTGCACA AATGAGGCCA
    GTGAACAAGT ATAGCTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292082.1
    CDS443..3643
    Translation

    Target ORF information:

    RefSeq Version XM_009293807.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D, like (sema6dl), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293807.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGGTGGC GAGAACTGAC GATTGACCTG CTGATACTCC TGCTGGTGGC GTCCCGTCTG 
    GATGCTGTGA GCTTTCCTGA GGACAACATC CCTTTAGACG TTGTGGACAG ACACTATGCA
    CGGCAGTACC CGGTGTTTCG TGGACGTCCC TCTGGTAACG AATCTCAGCA CAGACTGGAC
    TTTCAGCTGA TGACCCAGAT ACATGATACG CTGTTCATCG CGGGCAGGGA TCAGGTATAT
    TTGGTGAGTC TGAGGGAGTC CTACAGGAAC GAGATCATTC CATACAGGAA GCTGACGTGG
    CGGTCGGGGC AGGCTGACAG AGAGACGTGC GCCATGAAGG GAAAACACAG AGACGAGTGC
    CATAATTTCA TTAAAGTCCT GGTTCCGAGA AATGATGATC TCGTGTTCAT CTGCGGAACA
    AATGGTTTCA ATCCCATGTG CCGTTACTAC AGGTTGGATA ACCTGGAATT TGATGGGGAA
    GAGATCAGCG GTTTGGCCCG ATGCCCGTTT GACGCTAAGC AGACAAACGT TGCCCTGTTT
    GCCGATGGTA AGCTGTACTC TGCTACGGTG GCAGACTTCC TGGCTAGCGA TGCTGTGATT
    TATCGCAGTA TGGGAGATGG TTCTGCCCTT CGCACTATCA AGTATGACTC CAAGTGGTTG
    AAAGAGCCAC ATTTTCTTCA CGCTGTGGAC TACGGGGATT TTGTCTACTT CTTCTTTAGA
    GAAATTGCAG CAGAGCACAA CAACTTGGGA AAGGCGGTTT ACTCCAGAGT GGCTCGGATC
    TGTAAAAATG ACATGGGAGG GTCTCAGCGG GTGCTGGAAA AACACTGGAC ATCTTTTGTT
    AAGGCTCGGC TGAACTGTTC CGTTCCTGGA GAATCGTTTT TCTACTTTGA CGTCCTTCAG
    GCCATTACCG ATATCATCGA CATTAATGGG GTGCCTTCTG TGGTTGGAGT TTTCACCACT
    CAGTTAAACA GTATTCCTGG ATCTGCAGTG TGTGCCTTCT CCATGGTGGA CATTGAGAAA
    GCCTTCCGCG GCCGTTTCAA AGAGCAGAAG ACGCCAGATT CAGTGTGGAC GCCATTTCCA
    GAGGAGAAAC TGCCCAAGCC CAGACCTGGC TGCTGTGCAG GACACGGTTC AGCAGAGTCC
    TACAAAACCT CCATCGAGTT TCCAGACGAG ACCCTTCAAT TCATCAAGTC ACACCCACTC
    ATGGACGCCT CGGTCCCCTC CATTGGAGAC GAACCGTGGT TCACCAAAAC AAGAGTCAGG
    TACAGACTAA CAGCACTTGC AGTTGACAAC ACGGCAGGAC CCCATAAGAA TTACACAGTG
    GTTTTCATCG GCTCAGAAGC AGGCATCGTG CTAAAAGTTC TAGCCAAGAC ATCCCCGCTG
    TCTTTGAATG ACAGCGTCCT GCTGGAGGAA ATTGACGTTT TCAACCAGGC CAAGTGTTTA
    TCCAGCAATG AGGACGATCG GCGAATTTTG TCTTTCCACC TAGATAAAGA CAGTCACACA
    CTTTATGTGG CCTTTTCCAG CTGTGTGGTA AGAATACCAC TCAGCCGCTG CGAACGACAC
    AGTTCCTGTC AAAAGTCTTG TATTGCTTCA AGAGACCCAT ACTGCGGATG GATGTCACAT
    GGAGCTTGTG AACGTATTCC TGCAACTATG CAAAGTGGTT ATGAGCAAGA TGTTGAATAT
    GGCAACACTG CTCACCTCGG GGATTGTCAC GAATTTTTGG CCACTACATC AGCGCCAGAT
    TTCAAATCAT ATGGCGACCC AACCTCTGAC ATGGAGTTGC CATCTTCATC AGTCACAGTG
    CCCGCGACTT CTGAACCCAT ACAATCACCC CAAACCAACC CCACTCAGAC ACCACAACTC
    TTTGAACCCC ATGGTGACCC AGCCACTTCA CATTCTGTTG AGCATATACC AGGTGTTTGG
    GAAATACAAG CAGGTGAATC AAACCAGATG GTGCACATGA ACATCCTGAT CTCCTGTGTG
    TTAGCTGCGT TTCTGCTTGG TGCATTCATC GCAGGCATGG TTGTTTACTG CTATAGAGAT
    GCGTTTCTTC GCACTCCACG AAAGATCCAA AAAGATGCAG AGTCGGCACA ATCCTGCACT
    GATTCCACTG GCAGCTTTGC CAAGCTTAAC GGTCTGTTTG ACAGTCCTGT CAAAGAGTAC
    CAGCCTAATT TGGATACTCC CAAACTCTAC ACCAACCTGT TGAGCAATGG AAGGGAGGTG
    CCAACCACTG GAGAAACTAA GACTATGCTC CTGAGTGGGC AGCCACCGGA ATTAGCAGCA
    CTGCCTACGC CTGAATCCAC ACCTGTGCTG CAACAGAAGA GCCTCCAGCC CATTAAGAAC
    CAGTGGGAGA GAGCTCATGG AAAGATGAGT GGCTCCCGCA AAGATGCACC ACCCAAAAGC
    CCACAGTACC TTCCCTCTAG TCCACCTCCC CATTCTGCAA TCAATCCACA CATACCTAGT
    GCTGTGGTAC TGCCTAATGC CACGCATGAA AACAAAGCAG GCTTCAGCAC ACCAGAGGGG
    CCTCAAGCAG AAAAGAAGGT CCAGAATAGT GATCAGCATG GTTCCAAATC CGGACGTAAA
    GACCAGAGGC GAACAGTGGA TGCCAGGAAT ACGTTAAACG ACCTCTTGAA ACATCTAAAT
    GATAGCAACC CCAAGGCCAT CATGGTAGAG ATGCCCAAAT CTCGACAGCA CCTAATGCTG
    GAACCTATTG TAAGTCCAAC AGAGATTCCA CCTAAAGTCC CAAGTCGAGA GGCCTCCTTG
    TATTCTCCGT CTTCCTCGTT ACCAAGGAAC AGCCCGACCA AGAGAGTGGA TGTGCCGACA
    ACGCCAACGT CGCCCACAGG GCATATGGGA ACTCTTGAGA GACAGCGGTA TCACCGAGGC
    TCTTCCCAGC GACACTCCAT ATCCTCGCCG CCCAAAGGGC TGCACTCACC TAGCGGGGCC
    ATAGTGTCCC GGCAACCTAG CATGAACAGA GGTGGGTACA TACCCCCCAC TCCTACCCCA
    CCCACTAGAC TAGACTCTCA TGGGGTACCA ATGGCAATGA CGCCTTCCGT ATCCCGACAG
    AGCAGCTACA GTGGGCATGG ATCTTTACCA CGAACATCCA TTAAAAGGAC AGCATCGCTA
    AAGCCCGATG TGCCACCCAA ACCCAACGGC TTTGCACCAC AGACTGCACA AATGAGGCCA
    GTGAACAAGT ATAGCTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.