SEMA6D cDNA ORF clone, Bos taurus(cattle)

The following SEMA6D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEMA6D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa23347 XM_005211815.4
Latest version!
Bos taurus semaphorin 6D (SEMA6D), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa219557 XM_024997537.1
Latest version!
Bos taurus semaphorin 6D (SEMA6D), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa219558 XM_024997538.1
Latest version!
Bos taurus semaphorin 6D (SEMA6D), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa40864 NM_001191133.1
Latest version!
Bos taurus semaphorin 6D (SEMA6D), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa23344 XM_005211812.4
Latest version!
Bos taurus semaphorin 6D (SEMA6D), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa23345 XM_005211813.4
Latest version!
Bos taurus semaphorin 6D (SEMA6D), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa23346 XM_005211814.4
Latest version!
Bos taurus semaphorin 6D (SEMA6D), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa49888 XM_024997536.1
Latest version!
Bos taurus semaphorin 6D (SEMA6D), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa49888 XM_024997535.1
Latest version!
Bos taurus semaphorin 6D (SEMA6D), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa49888 XM_010809338.3
Latest version!
Bos taurus semaphorin 6D (SEMA6D), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa49888 XM_024997534.1
Latest version!
Bos taurus semaphorin 6D (SEMA6D), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa49889 XM_010809340.3
Latest version!
Bos taurus semaphorin 6D (SEMA6D), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa49890 XM_010809341.3
Latest version!
Bos taurus semaphorin 6D (SEMA6D), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa49891 XM_010809342.3
Latest version!
Bos taurus semaphorin 6D (SEMA6D), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa23346 XM_005211814.3 Bos taurus semaphorin 6D (SEMA6D), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa49889 XM_010809340.2 Bos taurus semaphorin 6D (SEMA6D), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa49890 XM_010809341.2 Bos taurus semaphorin 6D (SEMA6D), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa23347 XM_005211815.3 Bos taurus semaphorin 6D (SEMA6D), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa49891 XM_010809342.2 Bos taurus semaphorin 6D (SEMA6D), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa49888 XM_010809338.2 Bos taurus semaphorin 6D (SEMA6D), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa23344 XM_005211812.3 Bos taurus semaphorin 6D (SEMA6D), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa23345 XM_005211813.3 Bos taurus semaphorin 6D (SEMA6D), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa23347
    Clone ID Related Accession (Same CDS sequence) XM_005211815.3 , XM_005211815.4
    Accession Version XM_005211815.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product semaphorin-6D isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211815.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC
    AACACGGCCC ATCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAGGCC
    AACCAGATGG TCCACATGAA TGTCCTCATC ACCTGTGTCT TTGCAGCTTT TGTCTTGGGT
    GCGTTCATTG CAGGTGTGGC AGTCTACTGT TACCGTGACA TGTTTGTTCG GAAAAACAGA
    AAGATCCATA AAGATGCAGA ATCTGCCCAG TCATGCACAG ACTCCAGTGG AAGTTTTGCC
    AAGCTGAATG GTCTCTTTGA CAGCCCCGTC AAGGAATACC AACAGAATAT TGATTCTCCC
    AAATTATATA GTAACCTGCT GACCAGTCGG AAAGAGCTGC CACCCAATGG AGATACAAAA
    TCCATGGTCA TGGACCATCG AGGCCAACCT CCTGAGTTGG CTGCTCTCCC CACTCCTGAG
    TCTACGCCTG TGCTTCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC AGAAAAGGCC
    CATGGCCATG GAGCTTCAAG GAAAGAAACC CCCCAGTTTT TTCCTTCTAG TCCTCCACCA
    CATTCCCCAC TAAGTCACGG GCATATCCCC AGCGCCATTG TTCTTCCTAA TGCTACCCAT
    GACTACAACA CTTCTTTCTC AAACTCCAAT GCTCACAAAG CTGAAAAGAA GCTTCAAAAC
    ATTGACCATC CTCTTACAAA GTCATCCAGT AAAAGAGATC ACCGGCGTTC TGTGGATTCC
    AGAAATACCC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC
    ATCATGGGAG ACATCCAGAT GGCCCACCAG ACCCTAATGC TGGATCCCGT GGGACCTATG
    TCTGAAGTCC CGCCCAAGGT CCCTAACCGC GAGGCATCTC TCTACTCTCC TCCCTCGACT
    CTTCCCAGAA ATAGCCCAAC CAAGCGAGTG GATGTTCCCA CCACTCCTGG AGTTCCGATG
    ACTTCTTTGG AAAGACAGAG GGGTTACCAT AAAAATTCCT CCCAGAGGCA CTCTATATCG
    GCTATGCCTA AAAACTTAAG TTCACCAAAT GGTGTTTTGT TATCTAGACA GCCTAGTATG
    AACCGTGGAG GGTACGTGCC CACCCCTGCA GGGCCAAAGG TGGACTATAT TCAGGGAGCA
    CCAGTGAGTG CTCACCTACA GCCTTCCCTC TCCAGACAGA GCAGCTACAC CAGTAATGGC
    ACCCTTCCCC GGACGGGACT AAAGAGGACA CCGTCCTTAA AACCTGACGT GCCGCCAAAG
    CCTTCATTTG TTCCTCAAAC CACCTCCGTC AGACCACTGA ACAAATACAC TTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211872.1
    CDS345..3341
    Translation

    Target ORF information:

    RefSeq Version XM_005211815.3
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 6D (SEMA6D), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211815.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC
    AACACGGCCC ATCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAGGCC
    AACCAGATGG TCCACATGAA TGTCCTCATC ACCTGTGTCT TTGCAGCTTT TGTCTTGGGT
    GCGTTCATTG CAGGTGTGGC AGTCTACTGT TACCGTGACA TGTTTGTTCG GAAAAACAGA
    AAGATCCATA AAGATGCAGA ATCTGCCCAG TCATGCACAG ACTCCAGTGG AAGTTTTGCC
    AAGCTGAATG GTCTCTTTGA CAGCCCCGTC AAGGAATACC AACAGAATAT TGATTCTCCC
    AAATTATATA GTAACCTGCT GACCAGTCGG AAAGAGCTGC CACCCAATGG AGATACAAAA
    TCCATGGTCA TGGACCATCG AGGCCAACCT CCTGAGTTGG CTGCTCTCCC CACTCCTGAG
    TCTACGCCTG TGCTTCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC AGAAAAGGCC
    CATGGCCATG GAGCTTCAAG GAAAGAAACC CCCCAGTTTT TTCCTTCTAG TCCTCCACCA
    CATTCCCCAC TAAGTCACGG GCATATCCCC AGCGCCATTG TTCTTCCTAA TGCTACCCAT
    GACTACAACA CTTCTTTCTC AAACTCCAAT GCTCACAAAG CTGAAAAGAA GCTTCAAAAC
    ATTGACCATC CTCTTACAAA GTCATCCAGT AAAAGAGATC ACCGGCGTTC TGTGGATTCC
    AGAAATACCC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC
    ATCATGGGAG ACATCCAGAT GGCCCACCAG ACCCTAATGC TGGATCCCGT GGGACCTATG
    TCTGAAGTCC CGCCCAAGGT CCCTAACCGC GAGGCATCTC TCTACTCTCC TCCCTCGACT
    CTTCCCAGAA ATAGCCCAAC CAAGCGAGTG GATGTTCCCA CCACTCCTGG AGTTCCGATG
    ACTTCTTTGG AAAGACAGAG GGGTTACCAT AAAAATTCCT CCCAGAGGCA CTCTATATCG
    GCTATGCCTA AAAACTTAAG TTCACCAAAT GGTGTTTTGT TATCTAGACA GCCTAGTATG
    AACCGTGGAG GGTACGTGCC CACCCCTGCA GGGCCAAAGG TGGACTATAT TCAGGGAGCA
    CCAGTGAGTG CTCACCTACA GCCTTCCCTC TCCAGACAGA GCAGCTACAC CAGTAATGGC
    ACCCTTCCCC GGACGGGACT AAAGAGGACA CCGTCCTTAA AACCTGACGT GCCGCCAAAG
    CCTTCATTTG TTCCTCAAAC CACCTCCGTC AGACCACTGA ACAAATACAC TTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23347
    Clone ID Related Accession (Same CDS sequence) XM_005211815.3 , XM_005211815.4
    Accession Version XM_005211815.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product semaphorin-6D isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211815.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC
    AACACGGCCC ATCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAGGCC
    AACCAGATGG TCCACATGAA TGTCCTCATC ACCTGTGTCT TTGCAGCTTT TGTCTTGGGT
    GCGTTCATTG CAGGTGTGGC AGTCTACTGT TACCGTGACA TGTTTGTTCG GAAAAACAGA
    AAGATCCATA AAGATGCAGA ATCTGCCCAG TCATGCACAG ACTCCAGTGG AAGTTTTGCC
    AAGCTGAATG GTCTCTTTGA CAGCCCCGTC AAGGAATACC AACAGAATAT TGATTCTCCC
    AAATTATATA GTAACCTGCT GACCAGTCGG AAAGAGCTGC CACCCAATGG AGATACAAAA
    TCCATGGTCA TGGACCATCG AGGCCAACCT CCTGAGTTGG CTGCTCTCCC CACTCCTGAG
    TCTACGCCTG TGCTTCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC AGAAAAGGCC
    CATGGCCATG GAGCTTCAAG GAAAGAAACC CCCCAGTTTT TTCCTTCTAG TCCTCCACCA
    CATTCCCCAC TAAGTCACGG GCATATCCCC AGCGCCATTG TTCTTCCTAA TGCTACCCAT
    GACTACAACA CTTCTTTCTC AAACTCCAAT GCTCACAAAG CTGAAAAGAA GCTTCAAAAC
    ATTGACCATC CTCTTACAAA GTCATCCAGT AAAAGAGATC ACCGGCGTTC TGTGGATTCC
    AGAAATACCC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC
    ATCATGGGAG ACATCCAGAT GGCCCACCAG ACCCTAATGC TGGATCCCGT GGGACCTATG
    TCTGAAGTCC CGCCCAAGGT CCCTAACCGC GAGGCATCTC TCTACTCTCC TCCCTCGACT
    CTTCCCAGAA ATAGCCCAAC CAAGCGAGTG GATGTTCCCA CCACTCCTGG AGTTCCGATG
    ACTTCTTTGG AAAGACAGAG GGGTTACCAT AAAAATTCCT CCCAGAGGCA CTCTATATCG
    GCTATGCCTA AAAACTTAAG TTCACCAAAT GGTGTTTTGT TATCTAGACA GCCTAGTATG
    AACCGTGGAG GGTACGTGCC CACCCCTGCA GGGCCAAAGG TGGACTATAT TCAGGGAGCA
    CCAGTGAGTG CTCACCTACA GCCTTCCCTC TCCAGACAGA GCAGCTACAC CAGTAATGGC
    ACCCTTCCCC GGACGGGACT AAAGAGGACA CCGTCCTTAA AACCTGACGT GCCGCCAAAG
    CCTTCATTTG TTCCTCAAAC CACCTCCGTC AGACCACTGA ACAAATACAC TTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211872.1
    CDS180..3176
    Translation

    Target ORF information:

    RefSeq Version XM_005211815.4
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 6D (SEMA6D), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211815.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC
    AACACGGCCC ATCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAGGCC
    AACCAGATGG TCCACATGAA TGTCCTCATC ACCTGTGTCT TTGCAGCTTT TGTCTTGGGT
    GCGTTCATTG CAGGTGTGGC AGTCTACTGT TACCGTGACA TGTTTGTTCG GAAAAACAGA
    AAGATCCATA AAGATGCAGA ATCTGCCCAG TCATGCACAG ACTCCAGTGG AAGTTTTGCC
    AAGCTGAATG GTCTCTTTGA CAGCCCCGTC AAGGAATACC AACAGAATAT TGATTCTCCC
    AAATTATATA GTAACCTGCT GACCAGTCGG AAAGAGCTGC CACCCAATGG AGATACAAAA
    TCCATGGTCA TGGACCATCG AGGCCAACCT CCTGAGTTGG CTGCTCTCCC CACTCCTGAG
    TCTACGCCTG TGCTTCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC AGAAAAGGCC
    CATGGCCATG GAGCTTCAAG GAAAGAAACC CCCCAGTTTT TTCCTTCTAG TCCTCCACCA
    CATTCCCCAC TAAGTCACGG GCATATCCCC AGCGCCATTG TTCTTCCTAA TGCTACCCAT
    GACTACAACA CTTCTTTCTC AAACTCCAAT GCTCACAAAG CTGAAAAGAA GCTTCAAAAC
    ATTGACCATC CTCTTACAAA GTCATCCAGT AAAAGAGATC ACCGGCGTTC TGTGGATTCC
    AGAAATACCC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC
    ATCATGGGAG ACATCCAGAT GGCCCACCAG ACCCTAATGC TGGATCCCGT GGGACCTATG
    TCTGAAGTCC CGCCCAAGGT CCCTAACCGC GAGGCATCTC TCTACTCTCC TCCCTCGACT
    CTTCCCAGAA ATAGCCCAAC CAAGCGAGTG GATGTTCCCA CCACTCCTGG AGTTCCGATG
    ACTTCTTTGG AAAGACAGAG GGGTTACCAT AAAAATTCCT CCCAGAGGCA CTCTATATCG
    GCTATGCCTA AAAACTTAAG TTCACCAAAT GGTGTTTTGT TATCTAGACA GCCTAGTATG
    AACCGTGGAG GGTACGTGCC CACCCCTGCA GGGCCAAAGG TGGACTATAT TCAGGGAGCA
    CCAGTGAGTG CTCACCTACA GCCTTCCCTC TCCAGACAGA GCAGCTACAC CAGTAATGGC
    ACCCTTCCCC GGACGGGACT AAAGAGGACA CCGTCCTTAA AACCTGACGT GCCGCCAAAG
    CCTTCATTTG TTCCTCAAAC CACCTCCGTC AGACCACTGA ACAAATACAC TTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219557
    Clone ID Related Accession (Same CDS sequence) XM_024997537.1
    Accession Version XM_024997537.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product semaphorin-6D isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC
    AACACGGCCC ATCTAGGGGA CTGCCACGAA ATTTTGCCTA CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCTT CATCTTCTGT TACCACAATG
    GCAAGTATCC CAGAAATTAC ACCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACCAGC
    TCCCGGAAAT TTGTAGTTCA AGATGACCCA AACACTTCTG ATTTTACTGA TCCTTTATCA
    GGTGTACGGT GGGAAGTCCA GTCTGGAGAG GCCAACCAGA TGGTCCACAT GAATGTCCTC
    ATCACCTGTG TCTTTGCAGC TTTTGTCTTG GGTGCGTTCA TTGCAGGTGT GGCAGTCTAC
    TGTTACCGTG ACATGTTTGT TCGGAAAAAC AGAAAGATCC ATAAAGATGC AGAATCTGCC
    CAGTCATGCA CAGACTCCAG TGGAAGTTTT GCCAAGCTGA ATGGTCTCTT TGACAGCCCC
    GTCAAGGAAT ACCAACAGAA TATTGATTCT CCCAAATTAT ATAGTAACCT GCTGACCAGT
    CGGAAAGAGC TGCCACCCAA TGGAGATACA AAATCCATGG TCATGGACCA TCGAGGCCAA
    CCTCCTGAGT TGGCTGCTCT CCCCACTCCT GAGTCTACGC CTGTGCTTCA CCAGAAGACC
    CTGCAGGCCA TGAAGAGCCA CTCAGAAAAG GCCCATGGCC ATGGAGCTTC AAGGAAAGAA
    ACCCCCCAGT TTTTTCCTTC TAGTCCTCCA CCACATTCCC CACTAAGTCA CGGGCATATC
    CCCAGCGCCA TTGTTCTTCC TAATGCTACC CATGACTACA ACACTTCTTT CTCAAACTCC
    AATGCTCACA AAGCTGAAAA GAAGCTTCAA AACATTGACC ATCCTCTTAC AAAGTCATCC
    AGTAAAAGAG ATCACCGGCG TTCTGTGGAT TCCAGAAATA CCCTCAATGA TCTCCTGAAG
    CATCTAAATG ACCCAAACAG TAACCCCAAA GCCATCATGG GAGACATCCA GATGGCCCAC
    CAGACCCTAA TGCTGGATCC CGTGGGACCT ATGTCTGAAG TCCCGCCCAA GGTCCCTAAC
    CGCGAGGCAT CTCTCTACTC TCCTCCCTCG ACTCTTCCCA GAAATAGCCC AACCAAGCGA
    GTGGATGTTC CCACCACTCC TGGAGTTCCG ATGACTTCTT TGGAAAGACA GAGGGGTTAC
    CATAAAAATT CCTCCCAGAG GCACTCTATA TCGGCTATGC CTAAAAACTT AAGTTCACCA
    AATGGTGTTT TGTTATCTAG ACAGCCTAGT ATGAACCGTG GAGGGTACGT GCCCACCCCT
    GCAGGGCCAA AGGTGGACTA TATTCAGGGA GCACCAGTGA GTGCTCACCT ACAGCCTTCC
    CTCTCCAGAC AGAGCAGCTA CACCAGTAAT GGCACCCTTC CCCGGACGGG ACTAAAGAGG
    ACACCGTCCT TAAAACCTGA CGTGCCGCCA AAGCCTTCAT TTGTTCCTCA AACCACCTCC
    GTCAGACCAC TGAACAAATA CACTTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853305.1
    CDS180..3389
    Translation

    Target ORF information:

    RefSeq Version XM_024997537.1
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 6D (SEMA6D), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997537.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC
    AACACGGCCC ATCTAGGGGA CTGCCACGAA ATTTTGCCTA CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCTT CATCTTCTGT TACCACAATG
    GCAAGTATCC CAGAAATTAC ACCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACCAGC
    TCCCGGAAAT TTGTAGTTCA AGATGACCCA AACACTTCTG ATTTTACTGA TCCTTTATCA
    GGTGTACGGT GGGAAGTCCA GTCTGGAGAG GCCAACCAGA TGGTCCACAT GAATGTCCTC
    ATCACCTGTG TCTTTGCAGC TTTTGTCTTG GGTGCGTTCA TTGCAGGTGT GGCAGTCTAC
    TGTTACCGTG ACATGTTTGT TCGGAAAAAC AGAAAGATCC ATAAAGATGC AGAATCTGCC
    CAGTCATGCA CAGACTCCAG TGGAAGTTTT GCCAAGCTGA ATGGTCTCTT TGACAGCCCC
    GTCAAGGAAT ACCAACAGAA TATTGATTCT CCCAAATTAT ATAGTAACCT GCTGACCAGT
    CGGAAAGAGC TGCCACCCAA TGGAGATACA AAATCCATGG TCATGGACCA TCGAGGCCAA
    CCTCCTGAGT TGGCTGCTCT CCCCACTCCT GAGTCTACGC CTGTGCTTCA CCAGAAGACC
    CTGCAGGCCA TGAAGAGCCA CTCAGAAAAG GCCCATGGCC ATGGAGCTTC AAGGAAAGAA
    ACCCCCCAGT TTTTTCCTTC TAGTCCTCCA CCACATTCCC CACTAAGTCA CGGGCATATC
    CCCAGCGCCA TTGTTCTTCC TAATGCTACC CATGACTACA ACACTTCTTT CTCAAACTCC
    AATGCTCACA AAGCTGAAAA GAAGCTTCAA AACATTGACC ATCCTCTTAC AAAGTCATCC
    AGTAAAAGAG ATCACCGGCG TTCTGTGGAT TCCAGAAATA CCCTCAATGA TCTCCTGAAG
    CATCTAAATG ACCCAAACAG TAACCCCAAA GCCATCATGG GAGACATCCA GATGGCCCAC
    CAGACCCTAA TGCTGGATCC CGTGGGACCT ATGTCTGAAG TCCCGCCCAA GGTCCCTAAC
    CGCGAGGCAT CTCTCTACTC TCCTCCCTCG ACTCTTCCCA GAAATAGCCC AACCAAGCGA
    GTGGATGTTC CCACCACTCC TGGAGTTCCG ATGACTTCTT TGGAAAGACA GAGGGGTTAC
    CATAAAAATT CCTCCCAGAG GCACTCTATA TCGGCTATGC CTAAAAACTT AAGTTCACCA
    AATGGTGTTT TGTTATCTAG ACAGCCTAGT ATGAACCGTG GAGGGTACGT GCCCACCCCT
    GCAGGGCCAA AGGTGGACTA TATTCAGGGA GCACCAGTGA GTGCTCACCT ACAGCCTTCC
    CTCTCCAGAC AGAGCAGCTA CACCAGTAAT GGCACCCTTC CCCGGACGGG ACTAAAGAGG
    ACACCGTCCT TAAAACCTGA CGTGCCGCCA AAGCCTTCAT TTGTTCCTCA AACCACCTCC
    GTCAGACCAC TGAACAAATA CACTTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219558
    Clone ID Related Accession (Same CDS sequence) XM_024997538.1
    Accession Version XM_024997538.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3153bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product semaphorin-6D isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC
    AACACGGCCC ATCTAGGGGA CTGCCACGAC ATGGAGGTAT CTTCATCTTC TGTTACCACA
    ATGGCAAGTA TCCCAGAAAT TACACCTAAA GTGATTGATA CCTGGAGACC TAAACTGACC
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAACACTT CTGATTTTAC TGATCCTTTA
    TCAGGTGTAC GGTGGGAAGT CCAGTCTGGA GAGGCCAACC AGATGGTCCA CATGAATGTC
    CTCATCACCT GTGTCTTTGC AGCTTTTGTC TTGGGTGCGT TCATTGCAGG TGTGGCAGTC
    TACTGTTACC GTGACATGTT TGTTCGGAAA AACAGAAAGA TCCATAAAGA TGCAGAATCT
    GCCCAGTCAT GCACAGACTC CAGTGGAAGT TTTGCCAAGC TGAATGGTCT CTTTGACAGC
    CCCGTCAAGG AATACCAACA GAATATTGAT TCTCCCAAAT TATATAGTAA CCTGCTGACC
    AGTCGGAAAG AGCTGCCACC CAATGGAGAT ACAAAATCCA TGGTCATGGA CCATCGAGGC
    CAACCTCCTG AGTTGGCTGC TCTCCCCACT CCTGAGTCTA CGCCTGTGCT TCACCAGAAG
    ACCCTGCAGG CCATGAAGAG CCACTCAGAA AAGGCCCATG GCCATGGAGC TTCAAGGAAA
    GAAACCCCCC AGTTTTTTCC TTCTAGTCCT CCACCACATT CCCCACTAAG TCACGGGCAT
    ATCCCCAGCG CCATTGTTCT TCCTAATGCT ACCCATGACT ACAACACTTC TTTCTCAAAC
    TCCAATGCTC ACAAAGCTGA AAAGAAGCTT CAAAACATTG ACCATCCTCT TACAAAGTCA
    TCCAGTAAAA GAGATCACCG GCGTTCTGTG GATTCCAGAA ATACCCTCAA TGATCTCCTG
    AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCA TGGGAGACAT CCAGATGGCC
    CACCAGACCC TAATGCTGGA TCCCGTGGGA CCTATGTCTG AAGTCCCGCC CAAGGTCCCT
    AACCGCGAGG CATCTCTCTA CTCTCCTCCC TCGACTCTTC CCAGAAATAG CCCAACCAAG
    CGAGTGGATG TTCCCACCAC TCCTGGAGTT CCGATGACTT CTTTGGAAAG ACAGAGGGGT
    TACCATAAAA ATTCCTCCCA GAGGCACTCT ATATCGGCTA TGCCTAAAAA CTTAAGTTCA
    CCAAATGGTG TTTTGTTATC TAGACAGCCT AGTATGAACC GTGGAGGGTA CGTGCCCACC
    CCTGCAGGGC CAAAGGTGGA CTATATTCAG GGAGCACCAG TGAGTGCTCA CCTACAGCCT
    TCCCTCTCCA GACAGAGCAG CTACACCAGT AATGGCACCC TTCCCCGGAC GGGACTAAAG
    AGGACACCGT CCTTAAAACC TGACGTGCCG CCAAAGCCTT CATTTGTTCC TCAAACCACC
    TCCGTCAGAC CACTGAACAA ATACACTTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853306.1
    CDS180..3332
    Translation

    Target ORF information:

    RefSeq Version XM_024997538.1
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 6D (SEMA6D), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997538.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC
    AACACGGCCC ATCTAGGGGA CTGCCACGAC ATGGAGGTAT CTTCATCTTC TGTTACCACA
    ATGGCAAGTA TCCCAGAAAT TACACCTAAA GTGATTGATA CCTGGAGACC TAAACTGACC
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAACACTT CTGATTTTAC TGATCCTTTA
    TCAGGTGTAC GGTGGGAAGT CCAGTCTGGA GAGGCCAACC AGATGGTCCA CATGAATGTC
    CTCATCACCT GTGTCTTTGC AGCTTTTGTC TTGGGTGCGT TCATTGCAGG TGTGGCAGTC
    TACTGTTACC GTGACATGTT TGTTCGGAAA AACAGAAAGA TCCATAAAGA TGCAGAATCT
    GCCCAGTCAT GCACAGACTC CAGTGGAAGT TTTGCCAAGC TGAATGGTCT CTTTGACAGC
    CCCGTCAAGG AATACCAACA GAATATTGAT TCTCCCAAAT TATATAGTAA CCTGCTGACC
    AGTCGGAAAG AGCTGCCACC CAATGGAGAT ACAAAATCCA TGGTCATGGA CCATCGAGGC
    CAACCTCCTG AGTTGGCTGC TCTCCCCACT CCTGAGTCTA CGCCTGTGCT TCACCAGAAG
    ACCCTGCAGG CCATGAAGAG CCACTCAGAA AAGGCCCATG GCCATGGAGC TTCAAGGAAA
    GAAACCCCCC AGTTTTTTCC TTCTAGTCCT CCACCACATT CCCCACTAAG TCACGGGCAT
    ATCCCCAGCG CCATTGTTCT TCCTAATGCT ACCCATGACT ACAACACTTC TTTCTCAAAC
    TCCAATGCTC ACAAAGCTGA AAAGAAGCTT CAAAACATTG ACCATCCTCT TACAAAGTCA
    TCCAGTAAAA GAGATCACCG GCGTTCTGTG GATTCCAGAA ATACCCTCAA TGATCTCCTG
    AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCA TGGGAGACAT CCAGATGGCC
    CACCAGACCC TAATGCTGGA TCCCGTGGGA CCTATGTCTG AAGTCCCGCC CAAGGTCCCT
    AACCGCGAGG CATCTCTCTA CTCTCCTCCC TCGACTCTTC CCAGAAATAG CCCAACCAAG
    CGAGTGGATG TTCCCACCAC TCCTGGAGTT CCGATGACTT CTTTGGAAAG ACAGAGGGGT
    TACCATAAAA ATTCCTCCCA GAGGCACTCT ATATCGGCTA TGCCTAAAAA CTTAAGTTCA
    CCAAATGGTG TTTTGTTATC TAGACAGCCT AGTATGAACC GTGGAGGGTA CGTGCCCACC
    CCTGCAGGGC CAAAGGTGGA CTATATTCAG GGAGCACCAG TGAGTGCTCA CCTACAGCCT
    TCCCTCTCCA GACAGAGCAG CTACACCAGT AATGGCACCC TTCCCCGGAC GGGACTAAAG
    AGGACACCGT CCTTAAAACC TGACGTGCCG CCAAAGCCTT CATTTGTTCC TCAAACCACC
    TCCGTCAGAC CACTGAACAA ATACACTTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40864
    Clone ID Related Accession (Same CDS sequence) NM_001191133.1
    Accession Version NM_001191133.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product semaphorin-6D precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000010.1. On Jul 17, 2010 this sequence version replaced XM_001249780.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR5571258.3122.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC
    AACACGGCCC ATCTAGGGGA CTGCCACGAA ATTTTGCCTA CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCTT CATCTTCTGT TACCACAATG
    GCAAGTATCC CAGAAATTAC ACCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACCAGC
    TCCCGGAAAT TTGTAGTTCA AGATGACCCA AACACTTCTG ATTTTACTGA TCCTTTATCA
    GGTATCCCAA AGGGTGTACG GTGGGAAGTC CAGTCTGGAG AGGCCAACCA GATGGTCCAC
    ATGAATGTCC TCATCACCTG TGTCTTTGCA GCTTTTGTCT TGGGTGCGTT CATTGCAGGT
    GTGGCAGTCT ACTGTTACCG TGACATGTTT GTTCGGAAAA ACAGAAAGAT CCATAAAGAT
    GCAGAATCTG CCCAGTCATG CACAGACTCC AGTGGAAGTT TTGCCAAGCT GAATGGTCTC
    TTTGACAGCC CCGTCAAGGA ATACCAACAG AATATTGATT CTCCCAAATT ATATAGTAAC
    CTGCTGACCA GTCGGAAAGA GCTGCCACCC AATGGAGATA CAAAATCCAT GGTCATGGAC
    CATCGAGGCC AACCTCCTGA GTTGGCTGCT CTCCCCACTC CTGAGTCTAC GCCTGTGCTT
    CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCAGAAA AGGCCCATGG CCATGGAGCT
    TCAAGGAAAG AAACCCCCCA GTTTTTTCCT TCTAGTCCTC CACCACATTC CCCACTAAGT
    CACGGGCATA TCCCCAGCGC CATTGTTCTT CCTAATGCTA CCCATGACTA CAACACTTCT
    TTCTCAAACT CCAATGCTCA CAAAGCTGAA AAGAAGCTTC AAAACATTGA CCATCCTCTT
    ACAAAGTCAT CCAGTAAAAG AGATCACCGG CGTTCTGTGG ATTCCAGAAA TACCCTCAAT
    GATCTCCTGA AGCATCTAAA TGACCCAAAC AGTAACCCCA AAGCCATCAT GGGAGACATC
    CAGATGGCCC ACCAGACCCT AATGCTGGAT CCCGTGGGAC CTATGTCTGA AGTCCCGCCC
    AAGGTCCCTA ACCGCGAGGC ATCTCTCTAC TCTCCTCCCT CGACTCTTCC CAGAAATAGC
    CCAACCAAGC GAGTGGATGT TCCCACCACT CCTGGAGTTC CGATGACTTC TTTGGAAAGA
    CAGAGGGGTT ACCATAAAAA TTCCTCCCAG AGGCACTCTA TATCGGCTAT GCCTAAAAAC
    TTAAGTTCAC CAAATGGTGT TTTGTTATCT AGACAGCCTA GTATGAACCG TGGAGGGTAC
    GTGCCCACCC CTGCAGGGCC AAAGGTGGAC TATATTCAGG GAGCACCAGT GAGTGCTCAC
    CTACAGCCTT CCCTCTCCAG ACAGAGCAGC TACACCAGTA ATGGCACCCT TCCCCGGACG
    GGACTAAAGA GGACACCGTC CTTAAAACCT GACGTGCCGC CAAAGCCTTC ATTTGTTCCT
    CAAACCACCT CCGTCAGACC ACTGAACAAA TACACTTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178062.1
    CDS350..3571
    Translation

    Target ORF information:

    RefSeq Version NM_001191133.1
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 6D (SEMA6D), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191133.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC
    AACACGGCCC ATCTAGGGGA CTGCCACGAA ATTTTGCCTA CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCTT CATCTTCTGT TACCACAATG
    GCAAGTATCC CAGAAATTAC ACCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACCAGC
    TCCCGGAAAT TTGTAGTTCA AGATGACCCA AACACTTCTG ATTTTACTGA TCCTTTATCA
    GGTATCCCAA AGGGTGTACG GTGGGAAGTC CAGTCTGGAG AGGCCAACCA GATGGTCCAC
    ATGAATGTCC TCATCACCTG TGTCTTTGCA GCTTTTGTCT TGGGTGCGTT CATTGCAGGT
    GTGGCAGTCT ACTGTTACCG TGACATGTTT GTTCGGAAAA ACAGAAAGAT CCATAAAGAT
    GCAGAATCTG CCCAGTCATG CACAGACTCC AGTGGAAGTT TTGCCAAGCT GAATGGTCTC
    TTTGACAGCC CCGTCAAGGA ATACCAACAG AATATTGATT CTCCCAAATT ATATAGTAAC
    CTGCTGACCA GTCGGAAAGA GCTGCCACCC AATGGAGATA CAAAATCCAT GGTCATGGAC
    CATCGAGGCC AACCTCCTGA GTTGGCTGCT CTCCCCACTC CTGAGTCTAC GCCTGTGCTT
    CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCAGAAA AGGCCCATGG CCATGGAGCT
    TCAAGGAAAG AAACCCCCCA GTTTTTTCCT TCTAGTCCTC CACCACATTC CCCACTAAGT
    CACGGGCATA TCCCCAGCGC CATTGTTCTT CCTAATGCTA CCCATGACTA CAACACTTCT
    TTCTCAAACT CCAATGCTCA CAAAGCTGAA AAGAAGCTTC AAAACATTGA CCATCCTCTT
    ACAAAGTCAT CCAGTAAAAG AGATCACCGG CGTTCTGTGG ATTCCAGAAA TACCCTCAAT
    GATCTCCTGA AGCATCTAAA TGACCCAAAC AGTAACCCCA AAGCCATCAT GGGAGACATC
    CAGATGGCCC ACCAGACCCT AATGCTGGAT CCCGTGGGAC CTATGTCTGA AGTCCCGCCC
    AAGGTCCCTA ACCGCGAGGC ATCTCTCTAC TCTCCTCCCT CGACTCTTCC CAGAAATAGC
    CCAACCAAGC GAGTGGATGT TCCCACCACT CCTGGAGTTC CGATGACTTC TTTGGAAAGA
    CAGAGGGGTT ACCATAAAAA TTCCTCCCAG AGGCACTCTA TATCGGCTAT GCCTAAAAAC
    TTAAGTTCAC CAAATGGTGT TTTGTTATCT AGACAGCCTA GTATGAACCG TGGAGGGTAC
    GTGCCCACCC CTGCAGGGCC AAAGGTGGAC TATATTCAGG GAGCACCAGT GAGTGCTCAC
    CTACAGCCTT CCCTCTCCAG ACAGAGCAGC TACACCAGTA ATGGCACCCT TCCCCGGACG
    GGACTAAAGA GGACACCGTC CTTAAAACCT GACGTGCCGC CAAAGCCTTC ATTTGTTCCT
    CAAACCACCT CCGTCAGACC ACTGAACAAA TACACTTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23344
    Clone ID Related Accession (Same CDS sequence) XM_005211812.3 , XM_005211812.4
    Accession Version XM_005211812.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3093bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product semaphorin-6D isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211812.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC
    TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGGTGTAC GGTGGGAAGT CCAGTCTGGA GAGGCCAACC AGATGGTCCA CATGAATGTC
    CTCATCACCT GTGTCTTTGC AGCTTTTGTC TTGGGTGCGT TCATTGCAGG TGTGGCAGTC
    TACTGTTACC GTGACATGTT TGTTCGGAAA AACAGAAAGA TCCATAAAGA TGCAGAATCT
    GCCCAGTCAT GCACAGACTC CAGTGGAAGT TTTGCCAAGC TGAATGGTCT CTTTGACAGC
    CCCGTCAAGG AATACCAACA GAATATTGAT TCTCCCAAAT TATATAGTAA CCTGCTGACC
    AGTCGGAAAG AGCTGCCACC CAATGGAGAT ACAAAATCCA TGGTCATGGA CCATCGAGGC
    CAACCTCCTG AGTTGGCTGC TCTCCCCACT CCTGAGTCTA CGCCTGTGCT TCACCAGAAG
    ACCCTGCAGG CCATGAAGAG CCACTCAGAA AAGGCCCATG GCCATGGAGC TTCAAGGAAA
    GAAACCCCCC AGTTTTTTCC TTCTAGTCCT CCACCACATT CCCCACTAAG TCACGGGCAT
    ATCCCCAGCG CCATTGTTCT TCCTAATGCT ACCCATGACT ACAACACTTC TTTCTCAAAC
    TCCAATGCTC ACAAAGCTGA AAAGAAGCTT CAAAACATTG ACCATCCTCT TACAAAGTCA
    TCCAGTAAAA GAGATCACCG GCGTTCTGTG GATTCCAGAA ATACCCTCAA TGATCTCCTG
    AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCA TGGGAGACAT CCAGATGGCC
    CACCAGACCC TAATGCTGGA TCCCGTGGGA CCTATGTCTG AAGTCCCGCC CAAGGTCCCT
    AACCGCGAGG CATCTCTCTA CTCTCCTCCC TCGACTCTTC CCAGAAATAG CCCAACCAAG
    CGAGTGGATG TTCCCACCAC TCCTGGAGTT CCGATGACTT CTTTGGAAAG ACAGAGGGGT
    TACCATAAAA ATTCCTCCCA GAGGCACTCT ATATCGGCTA TGCCTAAAAA CTTAAGTTCA
    CCAAATGGTG TTTTGTTATC TAGACAGCCT AGTATGAACC GTGGAGGGTA CGTGCCCACC
    CCTGCAGGGC CAAAGGTGGA CTATATTCAG GGAGCACCAG TGAGTGCTCA CCTACAGCCT
    TCCCTCTCCA GACAGAGCAG CTACACCAGT AATGGCACCC TTCCCCGGAC GGGACTAAAG
    AGGACACCGT CCTTAAAACC TGACGTGCCG CCAAAGCCTT CATTTGTTCC TCAAACCACC
    TCCGTCAGAC CACTGAACAA ATACACTTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211869.1
    CDS345..3437
    Translation

    Target ORF information:

    RefSeq Version XM_005211812.3
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 6D (SEMA6D), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211812.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC
    TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGGTGTAC GGTGGGAAGT CCAGTCTGGA GAGGCCAACC AGATGGTCCA CATGAATGTC
    CTCATCACCT GTGTCTTTGC AGCTTTTGTC TTGGGTGCGT TCATTGCAGG TGTGGCAGTC
    TACTGTTACC GTGACATGTT TGTTCGGAAA AACAGAAAGA TCCATAAAGA TGCAGAATCT
    GCCCAGTCAT GCACAGACTC CAGTGGAAGT TTTGCCAAGC TGAATGGTCT CTTTGACAGC
    CCCGTCAAGG AATACCAACA GAATATTGAT TCTCCCAAAT TATATAGTAA CCTGCTGACC
    AGTCGGAAAG AGCTGCCACC CAATGGAGAT ACAAAATCCA TGGTCATGGA CCATCGAGGC
    CAACCTCCTG AGTTGGCTGC TCTCCCCACT CCTGAGTCTA CGCCTGTGCT TCACCAGAAG
    ACCCTGCAGG CCATGAAGAG CCACTCAGAA AAGGCCCATG GCCATGGAGC TTCAAGGAAA
    GAAACCCCCC AGTTTTTTCC TTCTAGTCCT CCACCACATT CCCCACTAAG TCACGGGCAT
    ATCCCCAGCG CCATTGTTCT TCCTAATGCT ACCCATGACT ACAACACTTC TTTCTCAAAC
    TCCAATGCTC ACAAAGCTGA AAAGAAGCTT CAAAACATTG ACCATCCTCT TACAAAGTCA
    TCCAGTAAAA GAGATCACCG GCGTTCTGTG GATTCCAGAA ATACCCTCAA TGATCTCCTG
    AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCA TGGGAGACAT CCAGATGGCC
    CACCAGACCC TAATGCTGGA TCCCGTGGGA CCTATGTCTG AAGTCCCGCC CAAGGTCCCT
    AACCGCGAGG CATCTCTCTA CTCTCCTCCC TCGACTCTTC CCAGAAATAG CCCAACCAAG
    CGAGTGGATG TTCCCACCAC TCCTGGAGTT CCGATGACTT CTTTGGAAAG ACAGAGGGGT
    TACCATAAAA ATTCCTCCCA GAGGCACTCT ATATCGGCTA TGCCTAAAAA CTTAAGTTCA
    CCAAATGGTG TTTTGTTATC TAGACAGCCT AGTATGAACC GTGGAGGGTA CGTGCCCACC
    CCTGCAGGGC CAAAGGTGGA CTATATTCAG GGAGCACCAG TGAGTGCTCA CCTACAGCCT
    TCCCTCTCCA GACAGAGCAG CTACACCAGT AATGGCACCC TTCCCCGGAC GGGACTAAAG
    AGGACACCGT CCTTAAAACC TGACGTGCCG CCAAAGCCTT CATTTGTTCC TCAAACCACC
    TCCGTCAGAC CACTGAACAA ATACACTTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23344
    Clone ID Related Accession (Same CDS sequence) XM_005211812.3 , XM_005211812.4
    Accession Version XM_005211812.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3093bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product semaphorin-6D isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211812.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC
    TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGGTGTAC GGTGGGAAGT CCAGTCTGGA GAGGCCAACC AGATGGTCCA CATGAATGTC
    CTCATCACCT GTGTCTTTGC AGCTTTTGTC TTGGGTGCGT TCATTGCAGG TGTGGCAGTC
    TACTGTTACC GTGACATGTT TGTTCGGAAA AACAGAAAGA TCCATAAAGA TGCAGAATCT
    GCCCAGTCAT GCACAGACTC CAGTGGAAGT TTTGCCAAGC TGAATGGTCT CTTTGACAGC
    CCCGTCAAGG AATACCAACA GAATATTGAT TCTCCCAAAT TATATAGTAA CCTGCTGACC
    AGTCGGAAAG AGCTGCCACC CAATGGAGAT ACAAAATCCA TGGTCATGGA CCATCGAGGC
    CAACCTCCTG AGTTGGCTGC TCTCCCCACT CCTGAGTCTA CGCCTGTGCT TCACCAGAAG
    ACCCTGCAGG CCATGAAGAG CCACTCAGAA AAGGCCCATG GCCATGGAGC TTCAAGGAAA
    GAAACCCCCC AGTTTTTTCC TTCTAGTCCT CCACCACATT CCCCACTAAG TCACGGGCAT
    ATCCCCAGCG CCATTGTTCT TCCTAATGCT ACCCATGACT ACAACACTTC TTTCTCAAAC
    TCCAATGCTC ACAAAGCTGA AAAGAAGCTT CAAAACATTG ACCATCCTCT TACAAAGTCA
    TCCAGTAAAA GAGATCACCG GCGTTCTGTG GATTCCAGAA ATACCCTCAA TGATCTCCTG
    AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCA TGGGAGACAT CCAGATGGCC
    CACCAGACCC TAATGCTGGA TCCCGTGGGA CCTATGTCTG AAGTCCCGCC CAAGGTCCCT
    AACCGCGAGG CATCTCTCTA CTCTCCTCCC TCGACTCTTC CCAGAAATAG CCCAACCAAG
    CGAGTGGATG TTCCCACCAC TCCTGGAGTT CCGATGACTT CTTTGGAAAG ACAGAGGGGT
    TACCATAAAA ATTCCTCCCA GAGGCACTCT ATATCGGCTA TGCCTAAAAA CTTAAGTTCA
    CCAAATGGTG TTTTGTTATC TAGACAGCCT AGTATGAACC GTGGAGGGTA CGTGCCCACC
    CCTGCAGGGC CAAAGGTGGA CTATATTCAG GGAGCACCAG TGAGTGCTCA CCTACAGCCT
    TCCCTCTCCA GACAGAGCAG CTACACCAGT AATGGCACCC TTCCCCGGAC GGGACTAAAG
    AGGACACCGT CCTTAAAACC TGACGTGCCG CCAAAGCCTT CATTTGTTCC TCAAACCACC
    TCCGTCAGAC CACTGAACAA ATACACTTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211869.1
    CDS180..3272
    Translation

    Target ORF information:

    RefSeq Version XM_005211812.4
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 6D (SEMA6D), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211812.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC
    TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGGTGTAC GGTGGGAAGT CCAGTCTGGA GAGGCCAACC AGATGGTCCA CATGAATGTC
    CTCATCACCT GTGTCTTTGC AGCTTTTGTC TTGGGTGCGT TCATTGCAGG TGTGGCAGTC
    TACTGTTACC GTGACATGTT TGTTCGGAAA AACAGAAAGA TCCATAAAGA TGCAGAATCT
    GCCCAGTCAT GCACAGACTC CAGTGGAAGT TTTGCCAAGC TGAATGGTCT CTTTGACAGC
    CCCGTCAAGG AATACCAACA GAATATTGAT TCTCCCAAAT TATATAGTAA CCTGCTGACC
    AGTCGGAAAG AGCTGCCACC CAATGGAGAT ACAAAATCCA TGGTCATGGA CCATCGAGGC
    CAACCTCCTG AGTTGGCTGC TCTCCCCACT CCTGAGTCTA CGCCTGTGCT TCACCAGAAG
    ACCCTGCAGG CCATGAAGAG CCACTCAGAA AAGGCCCATG GCCATGGAGC TTCAAGGAAA
    GAAACCCCCC AGTTTTTTCC TTCTAGTCCT CCACCACATT CCCCACTAAG TCACGGGCAT
    ATCCCCAGCG CCATTGTTCT TCCTAATGCT ACCCATGACT ACAACACTTC TTTCTCAAAC
    TCCAATGCTC ACAAAGCTGA AAAGAAGCTT CAAAACATTG ACCATCCTCT TACAAAGTCA
    TCCAGTAAAA GAGATCACCG GCGTTCTGTG GATTCCAGAA ATACCCTCAA TGATCTCCTG
    AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCA TGGGAGACAT CCAGATGGCC
    CACCAGACCC TAATGCTGGA TCCCGTGGGA CCTATGTCTG AAGTCCCGCC CAAGGTCCCT
    AACCGCGAGG CATCTCTCTA CTCTCCTCCC TCGACTCTTC CCAGAAATAG CCCAACCAAG
    CGAGTGGATG TTCCCACCAC TCCTGGAGTT CCGATGACTT CTTTGGAAAG ACAGAGGGGT
    TACCATAAAA ATTCCTCCCA GAGGCACTCT ATATCGGCTA TGCCTAAAAA CTTAAGTTCA
    CCAAATGGTG TTTTGTTATC TAGACAGCCT AGTATGAACC GTGGAGGGTA CGTGCCCACC
    CCTGCAGGGC CAAAGGTGGA CTATATTCAG GGAGCACCAG TGAGTGCTCA CCTACAGCCT
    TCCCTCTCCA GACAGAGCAG CTACACCAGT AATGGCACCC TTCCCCGGAC GGGACTAAAG
    AGGACACCGT CCTTAAAACC TGACGTGCCG CCAAAGCCTT CATTTGTTCC TCAAACCACC
    TCCGTCAGAC CACTGAACAA ATACACTTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23345
    Clone ID Related Accession (Same CDS sequence) XM_005211813.3 , XM_005211813.4
    Accession Version XM_005211813.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3054bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product semaphorin-6D isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211813.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC
    AACACGGCCC ATCTAGGGGA CTGCCACGAA ATTTTGCCTA CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGGTGTA CGGTGGGAAG TCCAGTCTGG AGAGGCCAAC
    CAGATGGTCC ACATGAATGT CCTCATCACC TGTGTCTTTG CAGCTTTTGT CTTGGGTGCG
    TTCATTGCAG GTGTGGCAGT CTACTGTTAC CGTGACATGT TTGTTCGGAA AAACAGAAAG
    ATCCATAAAG ATGCAGAATC TGCCCAGTCA TGCACAGACT CCAGTGGAAG TTTTGCCAAG
    CTGAATGGTC TCTTTGACAG CCCCGTCAAG GAATACCAAC AGAATATTGA TTCTCCCAAA
    TTATATAGTA ACCTGCTGAC CAGTCGGAAA GAGCTGCCAC CCAATGGAGA TACAAAATCC
    ATGGTCATGG ACCATCGAGG CCAACCTCCT GAGTTGGCTG CTCTCCCCAC TCCTGAGTCT
    ACGCCTGTGC TTCACCAGAA GACCCTGCAG GCCATGAAGA GCCACTCAGA AAAGGCCCAT
    GGCCATGGAG CTTCAAGGAA AGAAACCCCC CAGTTTTTTC CTTCTAGTCC TCCACCACAT
    TCCCCACTAA GTCACGGGCA TATCCCCAGC GCCATTGTTC TTCCTAATGC TACCCATGAC
    TACAACACTT CTTTCTCAAA CTCCAATGCT CACAAAGCTG AAAAGAAGCT TCAAAACATT
    GACCATCCTC TTACAAAGTC ATCCAGTAAA AGAGATCACC GGCGTTCTGT GGATTCCAGA
    AATACCCTCA ATGATCTCCT GAAGCATCTA AATGACCCAA ACAGTAACCC CAAAGCCATC
    ATGGGAGACA TCCAGATGGC CCACCAGACC CTAATGCTGG ATCCCGTGGG ACCTATGTCT
    GAAGTCCCGC CCAAGGTCCC TAACCGCGAG GCATCTCTCT ACTCTCCTCC CTCGACTCTT
    CCCAGAAATA GCCCAACCAA GCGAGTGGAT GTTCCCACCA CTCCTGGAGT TCCGATGACT
    TCTTTGGAAA GACAGAGGGG TTACCATAAA AATTCCTCCC AGAGGCACTC TATATCGGCT
    ATGCCTAAAA ACTTAAGTTC ACCAAATGGT GTTTTGTTAT CTAGACAGCC TAGTATGAAC
    CGTGGAGGGT ACGTGCCCAC CCCTGCAGGG CCAAAGGTGG ACTATATTCA GGGAGCACCA
    GTGAGTGCTC ACCTACAGCC TTCCCTCTCC AGACAGAGCA GCTACACCAG TAATGGCACC
    CTTCCCCGGA CGGGACTAAA GAGGACACCG TCCTTAAAAC CTGACGTGCC GCCAAAGCCT
    TCATTTGTTC CTCAAACCAC CTCCGTCAGA CCACTGAACA AATACACTTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211870.1
    CDS345..3398
    Translation

    Target ORF information:

    RefSeq Version XM_005211813.3
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 6D (SEMA6D), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211813.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC
    AACACGGCCC ATCTAGGGGA CTGCCACGAA ATTTTGCCTA CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGGTGTA CGGTGGGAAG TCCAGTCTGG AGAGGCCAAC
    CAGATGGTCC ACATGAATGT CCTCATCACC TGTGTCTTTG CAGCTTTTGT CTTGGGTGCG
    TTCATTGCAG GTGTGGCAGT CTACTGTTAC CGTGACATGT TTGTTCGGAA AAACAGAAAG
    ATCCATAAAG ATGCAGAATC TGCCCAGTCA TGCACAGACT CCAGTGGAAG TTTTGCCAAG
    CTGAATGGTC TCTTTGACAG CCCCGTCAAG GAATACCAAC AGAATATTGA TTCTCCCAAA
    TTATATAGTA ACCTGCTGAC CAGTCGGAAA GAGCTGCCAC CCAATGGAGA TACAAAATCC
    ATGGTCATGG ACCATCGAGG CCAACCTCCT GAGTTGGCTG CTCTCCCCAC TCCTGAGTCT
    ACGCCTGTGC TTCACCAGAA GACCCTGCAG GCCATGAAGA GCCACTCAGA AAAGGCCCAT
    GGCCATGGAG CTTCAAGGAA AGAAACCCCC CAGTTTTTTC CTTCTAGTCC TCCACCACAT
    TCCCCACTAA GTCACGGGCA TATCCCCAGC GCCATTGTTC TTCCTAATGC TACCCATGAC
    TACAACACTT CTTTCTCAAA CTCCAATGCT CACAAAGCTG AAAAGAAGCT TCAAAACATT
    GACCATCCTC TTACAAAGTC ATCCAGTAAA AGAGATCACC GGCGTTCTGT GGATTCCAGA
    AATACCCTCA ATGATCTCCT GAAGCATCTA AATGACCCAA ACAGTAACCC CAAAGCCATC
    ATGGGAGACA TCCAGATGGC CCACCAGACC CTAATGCTGG ATCCCGTGGG ACCTATGTCT
    GAAGTCCCGC CCAAGGTCCC TAACCGCGAG GCATCTCTCT ACTCTCCTCC CTCGACTCTT
    CCCAGAAATA GCCCAACCAA GCGAGTGGAT GTTCCCACCA CTCCTGGAGT TCCGATGACT
    TCTTTGGAAA GACAGAGGGG TTACCATAAA AATTCCTCCC AGAGGCACTC TATATCGGCT
    ATGCCTAAAA ACTTAAGTTC ACCAAATGGT GTTTTGTTAT CTAGACAGCC TAGTATGAAC
    CGTGGAGGGT ACGTGCCCAC CCCTGCAGGG CCAAAGGTGG ACTATATTCA GGGAGCACCA
    GTGAGTGCTC ACCTACAGCC TTCCCTCTCC AGACAGAGCA GCTACACCAG TAATGGCACC
    CTTCCCCGGA CGGGACTAAA GAGGACACCG TCCTTAAAAC CTGACGTGCC GCCAAAGCCT
    TCATTTGTTC CTCAAACCAC CTCCGTCAGA CCACTGAACA AATACACTTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23345
    Clone ID Related Accession (Same CDS sequence) XM_005211813.3 , XM_005211813.4
    Accession Version XM_005211813.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3054bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product semaphorin-6D isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211813.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC
    AACACGGCCC ATCTAGGGGA CTGCCACGAA ATTTTGCCTA CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGGTGTA CGGTGGGAAG TCCAGTCTGG AGAGGCCAAC
    CAGATGGTCC ACATGAATGT CCTCATCACC TGTGTCTTTG CAGCTTTTGT CTTGGGTGCG
    TTCATTGCAG GTGTGGCAGT CTACTGTTAC CGTGACATGT TTGTTCGGAA AAACAGAAAG
    ATCCATAAAG ATGCAGAATC TGCCCAGTCA TGCACAGACT CCAGTGGAAG TTTTGCCAAG
    CTGAATGGTC TCTTTGACAG CCCCGTCAAG GAATACCAAC AGAATATTGA TTCTCCCAAA
    TTATATAGTA ACCTGCTGAC CAGTCGGAAA GAGCTGCCAC CCAATGGAGA TACAAAATCC
    ATGGTCATGG ACCATCGAGG CCAACCTCCT GAGTTGGCTG CTCTCCCCAC TCCTGAGTCT
    ACGCCTGTGC TTCACCAGAA GACCCTGCAG GCCATGAAGA GCCACTCAGA AAAGGCCCAT
    GGCCATGGAG CTTCAAGGAA AGAAACCCCC CAGTTTTTTC CTTCTAGTCC TCCACCACAT
    TCCCCACTAA GTCACGGGCA TATCCCCAGC GCCATTGTTC TTCCTAATGC TACCCATGAC
    TACAACACTT CTTTCTCAAA CTCCAATGCT CACAAAGCTG AAAAGAAGCT TCAAAACATT
    GACCATCCTC TTACAAAGTC ATCCAGTAAA AGAGATCACC GGCGTTCTGT GGATTCCAGA
    AATACCCTCA ATGATCTCCT GAAGCATCTA AATGACCCAA ACAGTAACCC CAAAGCCATC
    ATGGGAGACA TCCAGATGGC CCACCAGACC CTAATGCTGG ATCCCGTGGG ACCTATGTCT
    GAAGTCCCGC CCAAGGTCCC TAACCGCGAG GCATCTCTCT ACTCTCCTCC CTCGACTCTT
    CCCAGAAATA GCCCAACCAA GCGAGTGGAT GTTCCCACCA CTCCTGGAGT TCCGATGACT
    TCTTTGGAAA GACAGAGGGG TTACCATAAA AATTCCTCCC AGAGGCACTC TATATCGGCT
    ATGCCTAAAA ACTTAAGTTC ACCAAATGGT GTTTTGTTAT CTAGACAGCC TAGTATGAAC
    CGTGGAGGGT ACGTGCCCAC CCCTGCAGGG CCAAAGGTGG ACTATATTCA GGGAGCACCA
    GTGAGTGCTC ACCTACAGCC TTCCCTCTCC AGACAGAGCA GCTACACCAG TAATGGCACC
    CTTCCCCGGA CGGGACTAAA GAGGACACCG TCCTTAAAAC CTGACGTGCC GCCAAAGCCT
    TCATTTGTTC CTCAAACCAC CTCCGTCAGA CCACTGAACA AATACACTTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211870.1
    CDS180..3233
    Translation

    Target ORF information:

    RefSeq Version XM_005211813.4
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 6D (SEMA6D), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211813.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC
    AACACGGCCC ATCTAGGGGA CTGCCACGAA ATTTTGCCTA CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGGTGTA CGGTGGGAAG TCCAGTCTGG AGAGGCCAAC
    CAGATGGTCC ACATGAATGT CCTCATCACC TGTGTCTTTG CAGCTTTTGT CTTGGGTGCG
    TTCATTGCAG GTGTGGCAGT CTACTGTTAC CGTGACATGT TTGTTCGGAA AAACAGAAAG
    ATCCATAAAG ATGCAGAATC TGCCCAGTCA TGCACAGACT CCAGTGGAAG TTTTGCCAAG
    CTGAATGGTC TCTTTGACAG CCCCGTCAAG GAATACCAAC AGAATATTGA TTCTCCCAAA
    TTATATAGTA ACCTGCTGAC CAGTCGGAAA GAGCTGCCAC CCAATGGAGA TACAAAATCC
    ATGGTCATGG ACCATCGAGG CCAACCTCCT GAGTTGGCTG CTCTCCCCAC TCCTGAGTCT
    ACGCCTGTGC TTCACCAGAA GACCCTGCAG GCCATGAAGA GCCACTCAGA AAAGGCCCAT
    GGCCATGGAG CTTCAAGGAA AGAAACCCCC CAGTTTTTTC CTTCTAGTCC TCCACCACAT
    TCCCCACTAA GTCACGGGCA TATCCCCAGC GCCATTGTTC TTCCTAATGC TACCCATGAC
    TACAACACTT CTTTCTCAAA CTCCAATGCT CACAAAGCTG AAAAGAAGCT TCAAAACATT
    GACCATCCTC TTACAAAGTC ATCCAGTAAA AGAGATCACC GGCGTTCTGT GGATTCCAGA
    AATACCCTCA ATGATCTCCT GAAGCATCTA AATGACCCAA ACAGTAACCC CAAAGCCATC
    ATGGGAGACA TCCAGATGGC CCACCAGACC CTAATGCTGG ATCCCGTGGG ACCTATGTCT
    GAAGTCCCGC CCAAGGTCCC TAACCGCGAG GCATCTCTCT ACTCTCCTCC CTCGACTCTT
    CCCAGAAATA GCCCAACCAA GCGAGTGGAT GTTCCCACCA CTCCTGGAGT TCCGATGACT
    TCTTTGGAAA GACAGAGGGG TTACCATAAA AATTCCTCCC AGAGGCACTC TATATCGGCT
    ATGCCTAAAA ACTTAAGTTC ACCAAATGGT GTTTTGTTAT CTAGACAGCC TAGTATGAAC
    CGTGGAGGGT ACGTGCCCAC CCCTGCAGGG CCAAAGGTGG ACTATATTCA GGGAGCACCA
    GTGAGTGCTC ACCTACAGCC TTCCCTCTCC AGACAGAGCA GCTACACCAG TAATGGCACC
    CTTCCCCGGA CGGGACTAAA GAGGACACCG TCCTTAAAAC CTGACGTGCC GCCAAAGCCT
    TCATTTGTTC CTCAAACCAC CTCCGTCAGA CCACTGAACA AATACACTTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23346
    Clone ID Related Accession (Same CDS sequence) XM_005211814.4 , XM_005211814.3
    Accession Version XM_005211814.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product semaphorin-6D isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211814.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC
    TGCCACGGTG TACGGTGGGA AGTCCAGTCT GGAGAGGCCA ACCAGATGGT CCACATGAAT
    GTCCTCATCA CCTGTGTCTT TGCAGCTTTT GTCTTGGGTG CGTTCATTGC AGGTGTGGCA
    GTCTACTGTT ACCGTGACAT GTTTGTTCGG AAAAACAGAA AGATCCATAA AGATGCAGAA
    TCTGCCCAGT CATGCACAGA CTCCAGTGGA AGTTTTGCCA AGCTGAATGG TCTCTTTGAC
    AGCCCCGTCA AGGAATACCA ACAGAATATT GATTCTCCCA AATTATATAG TAACCTGCTG
    ACCAGTCGGA AAGAGCTGCC ACCCAATGGA GATACAAAAT CCATGGTCAT GGACCATCGA
    GGCCAACCTC CTGAGTTGGC TGCTCTCCCC ACTCCTGAGT CTACGCCTGT GCTTCACCAG
    AAGACCCTGC AGGCCATGAA GAGCCACTCA GAAAAGGCCC ATGGCCATGG AGCTTCAAGG
    AAAGAAACCC CCCAGTTTTT TCCTTCTAGT CCTCCACCAC ATTCCCCACT AAGTCACGGG
    CATATCCCCA GCGCCATTGT TCTTCCTAAT GCTACCCATG ACTACAACAC TTCTTTCTCA
    AACTCCAATG CTCACAAAGC TGAAAAGAAG CTTCAAAACA TTGACCATCC TCTTACAAAG
    TCATCCAGTA AAAGAGATCA CCGGCGTTCT GTGGATTCCA GAAATACCCT CAATGATCTC
    CTGAAGCATC TAAATGACCC AAACAGTAAC CCCAAAGCCA TCATGGGAGA CATCCAGATG
    GCCCACCAGA CCCTAATGCT GGATCCCGTG GGACCTATGT CTGAAGTCCC GCCCAAGGTC
    CCTAACCGCG AGGCATCTCT CTACTCTCCT CCCTCGACTC TTCCCAGAAA TAGCCCAACC
    AAGCGAGTGG ATGTTCCCAC CACTCCTGGA GTTCCGATGA CTTCTTTGGA AAGACAGAGG
    GGTTACCATA AAAATTCCTC CCAGAGGCAC TCTATATCGG CTATGCCTAA AAACTTAAGT
    TCACCAAATG GTGTTTTGTT ATCTAGACAG CCTAGTATGA ACCGTGGAGG GTACGTGCCC
    ACCCCTGCAG GGCCAAAGGT GGACTATATT CAGGGAGCAC CAGTGAGTGC TCACCTACAG
    CCTTCCCTCT CCAGACAGAG CAGCTACACC AGTAATGGCA CCCTTCCCCG GACGGGACTA
    AAGAGGACAC CGTCCTTAAA ACCTGACGTG CCGCCAAAGC CTTCATTTGT TCCTCAAACC
    ACCTCCGTCA GACCACTGAA CAAATACACT TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211871.1
    CDS180..3215
    Translation

    Target ORF information:

    RefSeq Version XM_005211814.4
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 6D (SEMA6D), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211814.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC
    TGCCACGGTG TACGGTGGGA AGTCCAGTCT GGAGAGGCCA ACCAGATGGT CCACATGAAT
    GTCCTCATCA CCTGTGTCTT TGCAGCTTTT GTCTTGGGTG CGTTCATTGC AGGTGTGGCA
    GTCTACTGTT ACCGTGACAT GTTTGTTCGG AAAAACAGAA AGATCCATAA AGATGCAGAA
    TCTGCCCAGT CATGCACAGA CTCCAGTGGA AGTTTTGCCA AGCTGAATGG TCTCTTTGAC
    AGCCCCGTCA AGGAATACCA ACAGAATATT GATTCTCCCA AATTATATAG TAACCTGCTG
    ACCAGTCGGA AAGAGCTGCC ACCCAATGGA GATACAAAAT CCATGGTCAT GGACCATCGA
    GGCCAACCTC CTGAGTTGGC TGCTCTCCCC ACTCCTGAGT CTACGCCTGT GCTTCACCAG
    AAGACCCTGC AGGCCATGAA GAGCCACTCA GAAAAGGCCC ATGGCCATGG AGCTTCAAGG
    AAAGAAACCC CCCAGTTTTT TCCTTCTAGT CCTCCACCAC ATTCCCCACT AAGTCACGGG
    CATATCCCCA GCGCCATTGT TCTTCCTAAT GCTACCCATG ACTACAACAC TTCTTTCTCA
    AACTCCAATG CTCACAAAGC TGAAAAGAAG CTTCAAAACA TTGACCATCC TCTTACAAAG
    TCATCCAGTA AAAGAGATCA CCGGCGTTCT GTGGATTCCA GAAATACCCT CAATGATCTC
    CTGAAGCATC TAAATGACCC AAACAGTAAC CCCAAAGCCA TCATGGGAGA CATCCAGATG
    GCCCACCAGA CCCTAATGCT GGATCCCGTG GGACCTATGT CTGAAGTCCC GCCCAAGGTC
    CCTAACCGCG AGGCATCTCT CTACTCTCCT CCCTCGACTC TTCCCAGAAA TAGCCCAACC
    AAGCGAGTGG ATGTTCCCAC CACTCCTGGA GTTCCGATGA CTTCTTTGGA AAGACAGAGG
    GGTTACCATA AAAATTCCTC CCAGAGGCAC TCTATATCGG CTATGCCTAA AAACTTAAGT
    TCACCAAATG GTGTTTTGTT ATCTAGACAG CCTAGTATGA ACCGTGGAGG GTACGTGCCC
    ACCCCTGCAG GGCCAAAGGT GGACTATATT CAGGGAGCAC CAGTGAGTGC TCACCTACAG
    CCTTCCCTCT CCAGACAGAG CAGCTACACC AGTAATGGCA CCCTTCCCCG GACGGGACTA
    AAGAGGACAC CGTCCTTAAA ACCTGACGTG CCGCCAAAGC CTTCATTTGT TCCTCAAACC
    ACCTCCGTCA GACCACTGAA CAAATACACT TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23346
    Clone ID Related Accession (Same CDS sequence) XM_005211814.4 , XM_005211814.3
    Accession Version XM_005211814.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product semaphorin-6D isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211814.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC
    TGCCACGGTG TACGGTGGGA AGTCCAGTCT GGAGAGGCCA ACCAGATGGT CCACATGAAT
    GTCCTCATCA CCTGTGTCTT TGCAGCTTTT GTCTTGGGTG CGTTCATTGC AGGTGTGGCA
    GTCTACTGTT ACCGTGACAT GTTTGTTCGG AAAAACAGAA AGATCCATAA AGATGCAGAA
    TCTGCCCAGT CATGCACAGA CTCCAGTGGA AGTTTTGCCA AGCTGAATGG TCTCTTTGAC
    AGCCCCGTCA AGGAATACCA ACAGAATATT GATTCTCCCA AATTATATAG TAACCTGCTG
    ACCAGTCGGA AAGAGCTGCC ACCCAATGGA GATACAAAAT CCATGGTCAT GGACCATCGA
    GGCCAACCTC CTGAGTTGGC TGCTCTCCCC ACTCCTGAGT CTACGCCTGT GCTTCACCAG
    AAGACCCTGC AGGCCATGAA GAGCCACTCA GAAAAGGCCC ATGGCCATGG AGCTTCAAGG
    AAAGAAACCC CCCAGTTTTT TCCTTCTAGT CCTCCACCAC ATTCCCCACT AAGTCACGGG
    CATATCCCCA GCGCCATTGT TCTTCCTAAT GCTACCCATG ACTACAACAC TTCTTTCTCA
    AACTCCAATG CTCACAAAGC TGAAAAGAAG CTTCAAAACA TTGACCATCC TCTTACAAAG
    TCATCCAGTA AAAGAGATCA CCGGCGTTCT GTGGATTCCA GAAATACCCT CAATGATCTC
    CTGAAGCATC TAAATGACCC AAACAGTAAC CCCAAAGCCA TCATGGGAGA CATCCAGATG
    GCCCACCAGA CCCTAATGCT GGATCCCGTG GGACCTATGT CTGAAGTCCC GCCCAAGGTC
    CCTAACCGCG AGGCATCTCT CTACTCTCCT CCCTCGACTC TTCCCAGAAA TAGCCCAACC
    AAGCGAGTGG ATGTTCCCAC CACTCCTGGA GTTCCGATGA CTTCTTTGGA AAGACAGAGG
    GGTTACCATA AAAATTCCTC CCAGAGGCAC TCTATATCGG CTATGCCTAA AAACTTAAGT
    TCACCAAATG GTGTTTTGTT ATCTAGACAG CCTAGTATGA ACCGTGGAGG GTACGTGCCC
    ACCCCTGCAG GGCCAAAGGT GGACTATATT CAGGGAGCAC CAGTGAGTGC TCACCTACAG
    CCTTCCCTCT CCAGACAGAG CAGCTACACC AGTAATGGCA CCCTTCCCCG GACGGGACTA
    AAGAGGACAC CGTCCTTAAA ACCTGACGTG CCGCCAAAGC CTTCATTTGT TCCTCAAACC
    ACCTCCGTCA GACCACTGAA CAAATACACT TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211871.1
    CDS345..3380
    Translation

    Target ORF information:

    RefSeq Version XM_005211814.3
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 6D (SEMA6D), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211814.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC
    TGCCACGGTG TACGGTGGGA AGTCCAGTCT GGAGAGGCCA ACCAGATGGT CCACATGAAT
    GTCCTCATCA CCTGTGTCTT TGCAGCTTTT GTCTTGGGTG CGTTCATTGC AGGTGTGGCA
    GTCTACTGTT ACCGTGACAT GTTTGTTCGG AAAAACAGAA AGATCCATAA AGATGCAGAA
    TCTGCCCAGT CATGCACAGA CTCCAGTGGA AGTTTTGCCA AGCTGAATGG TCTCTTTGAC
    AGCCCCGTCA AGGAATACCA ACAGAATATT GATTCTCCCA AATTATATAG TAACCTGCTG
    ACCAGTCGGA AAGAGCTGCC ACCCAATGGA GATACAAAAT CCATGGTCAT GGACCATCGA
    GGCCAACCTC CTGAGTTGGC TGCTCTCCCC ACTCCTGAGT CTACGCCTGT GCTTCACCAG
    AAGACCCTGC AGGCCATGAA GAGCCACTCA GAAAAGGCCC ATGGCCATGG AGCTTCAAGG
    AAAGAAACCC CCCAGTTTTT TCCTTCTAGT CCTCCACCAC ATTCCCCACT AAGTCACGGG
    CATATCCCCA GCGCCATTGT TCTTCCTAAT GCTACCCATG ACTACAACAC TTCTTTCTCA
    AACTCCAATG CTCACAAAGC TGAAAAGAAG CTTCAAAACA TTGACCATCC TCTTACAAAG
    TCATCCAGTA AAAGAGATCA CCGGCGTTCT GTGGATTCCA GAAATACCCT CAATGATCTC
    CTGAAGCATC TAAATGACCC AAACAGTAAC CCCAAAGCCA TCATGGGAGA CATCCAGATG
    GCCCACCAGA CCCTAATGCT GGATCCCGTG GGACCTATGT CTGAAGTCCC GCCCAAGGTC
    CCTAACCGCG AGGCATCTCT CTACTCTCCT CCCTCGACTC TTCCCAGAAA TAGCCCAACC
    AAGCGAGTGG ATGTTCCCAC CACTCCTGGA GTTCCGATGA CTTCTTTGGA AAGACAGAGG
    GGTTACCATA AAAATTCCTC CCAGAGGCAC TCTATATCGG CTATGCCTAA AAACTTAAGT
    TCACCAAATG GTGTTTTGTT ATCTAGACAG CCTAGTATGA ACCGTGGAGG GTACGTGCCC
    ACCCCTGCAG GGCCAAAGGT GGACTATATT CAGGGAGCAC CAGTGAGTGC TCACCTACAG
    CCTTCCCTCT CCAGACAGAG CAGCTACACC AGTAATGGCA CCCTTCCCCG GACGGGACTA
    AAGAGGACAC CGTCCTTAAA ACCTGACGTG CCGCCAAAGC CTTCATTTGT TCCTCAAACC
    ACCTCCGTCA GACCACTGAA CAAATACACT TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49888
    Clone ID Related Accession (Same CDS sequence) XM_024997535.1 , XM_024997534.1 , XM_010809338.3 , XM_010809338.2 , XM_024997536.1
    Accession Version XM_024997535.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product semaphorin-6D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC
    TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA
    CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA GGCCAACCAG ATGGTCCACA TGAATGTCCT CATCACCTGT
    GTCTTTGCAG CTTTTGTCTT GGGTGCGTTC ATTGCAGGTG TGGCAGTCTA CTGTTACCGT
    GACATGTTTG TTCGGAAAAA CAGAAAGATC CATAAAGATG CAGAATCTGC CCAGTCATGC
    ACAGACTCCA GTGGAAGTTT TGCCAAGCTG AATGGTCTCT TTGACAGCCC CGTCAAGGAA
    TACCAACAGA ATATTGATTC TCCCAAATTA TATAGTAACC TGCTGACCAG TCGGAAAGAG
    CTGCCACCCA ATGGAGATAC AAAATCCATG GTCATGGACC ATCGAGGCCA ACCTCCTGAG
    TTGGCTGCTC TCCCCACTCC TGAGTCTACG CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCAGAAAA GGCCCATGGC CATGGAGCTT CAAGGAAAGA AACCCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCACATTCC CCACTAAGTC ACGGGCATAT CCCCAGCGCC
    ATTGTTCTTC CTAATGCTAC CCATGACTAC AACACTTCTT TCTCAAACTC CAATGCTCAC
    AAAGCTGAAA AGAAGCTTCA AAACATTGAC CATCCTCTTA CAAAGTCATC CAGTAAAAGA
    GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCATG GGAGACATCC AGATGGCCCA CCAGACCCTA
    ATGCTGGATC CCGTGGGACC TATGTCTGAA GTCCCGCCCA AGGTCCCTAA CCGCGAGGCA
    TCTCTCTACT CTCCTCCCTC GACTCTTCCC AGAAATAGCC CAACCAAGCG AGTGGATGTT
    CCCACCACTC CTGGAGTTCC GATGACTTCT TTGGAAAGAC AGAGGGGTTA CCATAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCGGCTATG CCTAAAAACT TAAGTTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCTAG TATGAACCGT GGAGGGTACG TGCCCACCCC TGCAGGGCCA
    AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGCTCACC TACAGCCTTC CCTCTCCAGA
    CAGAGCAGCT ACACCAGTAA TGGCACCCTT CCCCGGACGG GACTAAAGAG GACACCGTCC
    TTAAAACCTG ACGTGCCGCC AAAGCCTTCA TTTGTTCCTC AAACCACCTC CGTCAGACCA
    CTGAACAAAT ACACTTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853303.1
    CDS135..3395
    Translation

    Target ORF information:

    RefSeq Version XM_024997535.1
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 6D (SEMA6D), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997535.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC
    TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA
    CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA GGCCAACCAG ATGGTCCACA TGAATGTCCT CATCACCTGT
    GTCTTTGCAG CTTTTGTCTT GGGTGCGTTC ATTGCAGGTG TGGCAGTCTA CTGTTACCGT
    GACATGTTTG TTCGGAAAAA CAGAAAGATC CATAAAGATG CAGAATCTGC CCAGTCATGC
    ACAGACTCCA GTGGAAGTTT TGCCAAGCTG AATGGTCTCT TTGACAGCCC CGTCAAGGAA
    TACCAACAGA ATATTGATTC TCCCAAATTA TATAGTAACC TGCTGACCAG TCGGAAAGAG
    CTGCCACCCA ATGGAGATAC AAAATCCATG GTCATGGACC ATCGAGGCCA ACCTCCTGAG
    TTGGCTGCTC TCCCCACTCC TGAGTCTACG CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCAGAAAA GGCCCATGGC CATGGAGCTT CAAGGAAAGA AACCCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCACATTCC CCACTAAGTC ACGGGCATAT CCCCAGCGCC
    ATTGTTCTTC CTAATGCTAC CCATGACTAC AACACTTCTT TCTCAAACTC CAATGCTCAC
    AAAGCTGAAA AGAAGCTTCA AAACATTGAC CATCCTCTTA CAAAGTCATC CAGTAAAAGA
    GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCATG GGAGACATCC AGATGGCCCA CCAGACCCTA
    ATGCTGGATC CCGTGGGACC TATGTCTGAA GTCCCGCCCA AGGTCCCTAA CCGCGAGGCA
    TCTCTCTACT CTCCTCCCTC GACTCTTCCC AGAAATAGCC CAACCAAGCG AGTGGATGTT
    CCCACCACTC CTGGAGTTCC GATGACTTCT TTGGAAAGAC AGAGGGGTTA CCATAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCGGCTATG CCTAAAAACT TAAGTTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCTAG TATGAACCGT GGAGGGTACG TGCCCACCCC TGCAGGGCCA
    AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGCTCACC TACAGCCTTC CCTCTCCAGA
    CAGAGCAGCT ACACCAGTAA TGGCACCCTT CCCCGGACGG GACTAAAGAG GACACCGTCC
    TTAAAACCTG ACGTGCCGCC AAAGCCTTCA TTTGTTCCTC AAACCACCTC CGTCAGACCA
    CTGAACAAAT ACACTTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49888
    Clone ID Related Accession (Same CDS sequence) XM_024997535.1 , XM_024997534.1 , XM_010809338.3 , XM_010809338.2 , XM_024997536.1
    Accession Version XM_024997534.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product semaphorin-6D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC
    TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA
    CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA GGCCAACCAG ATGGTCCACA TGAATGTCCT CATCACCTGT
    GTCTTTGCAG CTTTTGTCTT GGGTGCGTTC ATTGCAGGTG TGGCAGTCTA CTGTTACCGT
    GACATGTTTG TTCGGAAAAA CAGAAAGATC CATAAAGATG CAGAATCTGC CCAGTCATGC
    ACAGACTCCA GTGGAAGTTT TGCCAAGCTG AATGGTCTCT TTGACAGCCC CGTCAAGGAA
    TACCAACAGA ATATTGATTC TCCCAAATTA TATAGTAACC TGCTGACCAG TCGGAAAGAG
    CTGCCACCCA ATGGAGATAC AAAATCCATG GTCATGGACC ATCGAGGCCA ACCTCCTGAG
    TTGGCTGCTC TCCCCACTCC TGAGTCTACG CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCAGAAAA GGCCCATGGC CATGGAGCTT CAAGGAAAGA AACCCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCACATTCC CCACTAAGTC ACGGGCATAT CCCCAGCGCC
    ATTGTTCTTC CTAATGCTAC CCATGACTAC AACACTTCTT TCTCAAACTC CAATGCTCAC
    AAAGCTGAAA AGAAGCTTCA AAACATTGAC CATCCTCTTA CAAAGTCATC CAGTAAAAGA
    GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCATG GGAGACATCC AGATGGCCCA CCAGACCCTA
    ATGCTGGATC CCGTGGGACC TATGTCTGAA GTCCCGCCCA AGGTCCCTAA CCGCGAGGCA
    TCTCTCTACT CTCCTCCCTC GACTCTTCCC AGAAATAGCC CAACCAAGCG AGTGGATGTT
    CCCACCACTC CTGGAGTTCC GATGACTTCT TTGGAAAGAC AGAGGGGTTA CCATAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCGGCTATG CCTAAAAACT TAAGTTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCTAG TATGAACCGT GGAGGGTACG TGCCCACCCC TGCAGGGCCA
    AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGCTCACC TACAGCCTTC CCTCTCCAGA
    CAGAGCAGCT ACACCAGTAA TGGCACCCTT CCCCGGACGG GACTAAAGAG GACACCGTCC
    TTAAAACCTG ACGTGCCGCC AAAGCCTTCA TTTGTTCCTC AAACCACCTC CGTCAGACCA
    CTGAACAAAT ACACTTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853302.1
    CDS134..3394
    Translation

    Target ORF information:

    RefSeq Version XM_024997534.1
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 6D (SEMA6D), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997534.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC
    TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA
    CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA GGCCAACCAG ATGGTCCACA TGAATGTCCT CATCACCTGT
    GTCTTTGCAG CTTTTGTCTT GGGTGCGTTC ATTGCAGGTG TGGCAGTCTA CTGTTACCGT
    GACATGTTTG TTCGGAAAAA CAGAAAGATC CATAAAGATG CAGAATCTGC CCAGTCATGC
    ACAGACTCCA GTGGAAGTTT TGCCAAGCTG AATGGTCTCT TTGACAGCCC CGTCAAGGAA
    TACCAACAGA ATATTGATTC TCCCAAATTA TATAGTAACC TGCTGACCAG TCGGAAAGAG
    CTGCCACCCA ATGGAGATAC AAAATCCATG GTCATGGACC ATCGAGGCCA ACCTCCTGAG
    TTGGCTGCTC TCCCCACTCC TGAGTCTACG CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCAGAAAA GGCCCATGGC CATGGAGCTT CAAGGAAAGA AACCCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCACATTCC CCACTAAGTC ACGGGCATAT CCCCAGCGCC
    ATTGTTCTTC CTAATGCTAC CCATGACTAC AACACTTCTT TCTCAAACTC CAATGCTCAC
    AAAGCTGAAA AGAAGCTTCA AAACATTGAC CATCCTCTTA CAAAGTCATC CAGTAAAAGA
    GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCATG GGAGACATCC AGATGGCCCA CCAGACCCTA
    ATGCTGGATC CCGTGGGACC TATGTCTGAA GTCCCGCCCA AGGTCCCTAA CCGCGAGGCA
    TCTCTCTACT CTCCTCCCTC GACTCTTCCC AGAAATAGCC CAACCAAGCG AGTGGATGTT
    CCCACCACTC CTGGAGTTCC GATGACTTCT TTGGAAAGAC AGAGGGGTTA CCATAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCGGCTATG CCTAAAAACT TAAGTTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCTAG TATGAACCGT GGAGGGTACG TGCCCACCCC TGCAGGGCCA
    AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGCTCACC TACAGCCTTC CCTCTCCAGA
    CAGAGCAGCT ACACCAGTAA TGGCACCCTT CCCCGGACGG GACTAAAGAG GACACCGTCC
    TTAAAACCTG ACGTGCCGCC AAAGCCTTCA TTTGTTCCTC AAACCACCTC CGTCAGACCA
    CTGAACAAAT ACACTTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49888
    Clone ID Related Accession (Same CDS sequence) XM_024997535.1 , XM_024997534.1 , XM_010809338.3 , XM_010809338.2 , XM_024997536.1
    Accession Version XM_010809338.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product semaphorin-6D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809338.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC
    TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA
    CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA GGCCAACCAG ATGGTCCACA TGAATGTCCT CATCACCTGT
    GTCTTTGCAG CTTTTGTCTT GGGTGCGTTC ATTGCAGGTG TGGCAGTCTA CTGTTACCGT
    GACATGTTTG TTCGGAAAAA CAGAAAGATC CATAAAGATG CAGAATCTGC CCAGTCATGC
    ACAGACTCCA GTGGAAGTTT TGCCAAGCTG AATGGTCTCT TTGACAGCCC CGTCAAGGAA
    TACCAACAGA ATATTGATTC TCCCAAATTA TATAGTAACC TGCTGACCAG TCGGAAAGAG
    CTGCCACCCA ATGGAGATAC AAAATCCATG GTCATGGACC ATCGAGGCCA ACCTCCTGAG
    TTGGCTGCTC TCCCCACTCC TGAGTCTACG CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCAGAAAA GGCCCATGGC CATGGAGCTT CAAGGAAAGA AACCCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCACATTCC CCACTAAGTC ACGGGCATAT CCCCAGCGCC
    ATTGTTCTTC CTAATGCTAC CCATGACTAC AACACTTCTT TCTCAAACTC CAATGCTCAC
    AAAGCTGAAA AGAAGCTTCA AAACATTGAC CATCCTCTTA CAAAGTCATC CAGTAAAAGA
    GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCATG GGAGACATCC AGATGGCCCA CCAGACCCTA
    ATGCTGGATC CCGTGGGACC TATGTCTGAA GTCCCGCCCA AGGTCCCTAA CCGCGAGGCA
    TCTCTCTACT CTCCTCCCTC GACTCTTCCC AGAAATAGCC CAACCAAGCG AGTGGATGTT
    CCCACCACTC CTGGAGTTCC GATGACTTCT TTGGAAAGAC AGAGGGGTTA CCATAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCGGCTATG CCTAAAAACT TAAGTTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCTAG TATGAACCGT GGAGGGTACG TGCCCACCCC TGCAGGGCCA
    AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGCTCACC TACAGCCTTC CCTCTCCAGA
    CAGAGCAGCT ACACCAGTAA TGGCACCCTT CCCCGGACGG GACTAAAGAG GACACCGTCC
    TTAAAACCTG ACGTGCCGCC AAAGCCTTCA TTTGTTCCTC AAACCACCTC CGTCAGACCA
    CTGAACAAAT ACACTTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807640.1
    CDS180..3440
    Translation

    Target ORF information:

    RefSeq Version XM_010809338.3
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 6D (SEMA6D), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809338.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC
    TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA
    CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA GGCCAACCAG ATGGTCCACA TGAATGTCCT CATCACCTGT
    GTCTTTGCAG CTTTTGTCTT GGGTGCGTTC ATTGCAGGTG TGGCAGTCTA CTGTTACCGT
    GACATGTTTG TTCGGAAAAA CAGAAAGATC CATAAAGATG CAGAATCTGC CCAGTCATGC
    ACAGACTCCA GTGGAAGTTT TGCCAAGCTG AATGGTCTCT TTGACAGCCC CGTCAAGGAA
    TACCAACAGA ATATTGATTC TCCCAAATTA TATAGTAACC TGCTGACCAG TCGGAAAGAG
    CTGCCACCCA ATGGAGATAC AAAATCCATG GTCATGGACC ATCGAGGCCA ACCTCCTGAG
    TTGGCTGCTC TCCCCACTCC TGAGTCTACG CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCAGAAAA GGCCCATGGC CATGGAGCTT CAAGGAAAGA AACCCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCACATTCC CCACTAAGTC ACGGGCATAT CCCCAGCGCC
    ATTGTTCTTC CTAATGCTAC CCATGACTAC AACACTTCTT TCTCAAACTC CAATGCTCAC
    AAAGCTGAAA AGAAGCTTCA AAACATTGAC CATCCTCTTA CAAAGTCATC CAGTAAAAGA
    GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCATG GGAGACATCC AGATGGCCCA CCAGACCCTA
    ATGCTGGATC CCGTGGGACC TATGTCTGAA GTCCCGCCCA AGGTCCCTAA CCGCGAGGCA
    TCTCTCTACT CTCCTCCCTC GACTCTTCCC AGAAATAGCC CAACCAAGCG AGTGGATGTT
    CCCACCACTC CTGGAGTTCC GATGACTTCT TTGGAAAGAC AGAGGGGTTA CCATAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCGGCTATG CCTAAAAACT TAAGTTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCTAG TATGAACCGT GGAGGGTACG TGCCCACCCC TGCAGGGCCA
    AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGCTCACC TACAGCCTTC CCTCTCCAGA
    CAGAGCAGCT ACACCAGTAA TGGCACCCTT CCCCGGACGG GACTAAAGAG GACACCGTCC
    TTAAAACCTG ACGTGCCGCC AAAGCCTTCA TTTGTTCCTC AAACCACCTC CGTCAGACCA
    CTGAACAAAT ACACTTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49888
    Clone ID Related Accession (Same CDS sequence) XM_024997535.1 , XM_024997534.1 , XM_010809338.3 , XM_010809338.2 , XM_024997536.1
    Accession Version XM_010809338.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product semaphorin-6D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809338.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC
    TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA
    CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA GGCCAACCAG ATGGTCCACA TGAATGTCCT CATCACCTGT
    GTCTTTGCAG CTTTTGTCTT GGGTGCGTTC ATTGCAGGTG TGGCAGTCTA CTGTTACCGT
    GACATGTTTG TTCGGAAAAA CAGAAAGATC CATAAAGATG CAGAATCTGC CCAGTCATGC
    ACAGACTCCA GTGGAAGTTT TGCCAAGCTG AATGGTCTCT TTGACAGCCC CGTCAAGGAA
    TACCAACAGA ATATTGATTC TCCCAAATTA TATAGTAACC TGCTGACCAG TCGGAAAGAG
    CTGCCACCCA ATGGAGATAC AAAATCCATG GTCATGGACC ATCGAGGCCA ACCTCCTGAG
    TTGGCTGCTC TCCCCACTCC TGAGTCTACG CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCAGAAAA GGCCCATGGC CATGGAGCTT CAAGGAAAGA AACCCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCACATTCC CCACTAAGTC ACGGGCATAT CCCCAGCGCC
    ATTGTTCTTC CTAATGCTAC CCATGACTAC AACACTTCTT TCTCAAACTC CAATGCTCAC
    AAAGCTGAAA AGAAGCTTCA AAACATTGAC CATCCTCTTA CAAAGTCATC CAGTAAAAGA
    GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCATG GGAGACATCC AGATGGCCCA CCAGACCCTA
    ATGCTGGATC CCGTGGGACC TATGTCTGAA GTCCCGCCCA AGGTCCCTAA CCGCGAGGCA
    TCTCTCTACT CTCCTCCCTC GACTCTTCCC AGAAATAGCC CAACCAAGCG AGTGGATGTT
    CCCACCACTC CTGGAGTTCC GATGACTTCT TTGGAAAGAC AGAGGGGTTA CCATAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCGGCTATG CCTAAAAACT TAAGTTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCTAG TATGAACCGT GGAGGGTACG TGCCCACCCC TGCAGGGCCA
    AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGCTCACC TACAGCCTTC CCTCTCCAGA
    CAGAGCAGCT ACACCAGTAA TGGCACCCTT CCCCGGACGG GACTAAAGAG GACACCGTCC
    TTAAAACCTG ACGTGCCGCC AAAGCCTTCA TTTGTTCCTC AAACCACCTC CGTCAGACCA
    CTGAACAAAT ACACTTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807640.1
    CDS345..3605
    Translation

    Target ORF information:

    RefSeq Version XM_010809338.2
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 6D (SEMA6D), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809338.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC
    TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA
    CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA GGCCAACCAG ATGGTCCACA TGAATGTCCT CATCACCTGT
    GTCTTTGCAG CTTTTGTCTT GGGTGCGTTC ATTGCAGGTG TGGCAGTCTA CTGTTACCGT
    GACATGTTTG TTCGGAAAAA CAGAAAGATC CATAAAGATG CAGAATCTGC CCAGTCATGC
    ACAGACTCCA GTGGAAGTTT TGCCAAGCTG AATGGTCTCT TTGACAGCCC CGTCAAGGAA
    TACCAACAGA ATATTGATTC TCCCAAATTA TATAGTAACC TGCTGACCAG TCGGAAAGAG
    CTGCCACCCA ATGGAGATAC AAAATCCATG GTCATGGACC ATCGAGGCCA ACCTCCTGAG
    TTGGCTGCTC TCCCCACTCC TGAGTCTACG CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCAGAAAA GGCCCATGGC CATGGAGCTT CAAGGAAAGA AACCCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCACATTCC CCACTAAGTC ACGGGCATAT CCCCAGCGCC
    ATTGTTCTTC CTAATGCTAC CCATGACTAC AACACTTCTT TCTCAAACTC CAATGCTCAC
    AAAGCTGAAA AGAAGCTTCA AAACATTGAC CATCCTCTTA CAAAGTCATC CAGTAAAAGA
    GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCATG GGAGACATCC AGATGGCCCA CCAGACCCTA
    ATGCTGGATC CCGTGGGACC TATGTCTGAA GTCCCGCCCA AGGTCCCTAA CCGCGAGGCA
    TCTCTCTACT CTCCTCCCTC GACTCTTCCC AGAAATAGCC CAACCAAGCG AGTGGATGTT
    CCCACCACTC CTGGAGTTCC GATGACTTCT TTGGAAAGAC AGAGGGGTTA CCATAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCGGCTATG CCTAAAAACT TAAGTTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCTAG TATGAACCGT GGAGGGTACG TGCCCACCCC TGCAGGGCCA
    AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGCTCACC TACAGCCTTC CCTCTCCAGA
    CAGAGCAGCT ACACCAGTAA TGGCACCCTT CCCCGGACGG GACTAAAGAG GACACCGTCC
    TTAAAACCTG ACGTGCCGCC AAAGCCTTCA TTTGTTCCTC AAACCACCTC CGTCAGACCA
    CTGAACAAAT ACACTTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49888
    Clone ID Related Accession (Same CDS sequence) XM_024997535.1 , XM_024997534.1 , XM_010809338.3 , XM_010809338.2 , XM_024997536.1
    Accession Version XM_024997536.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product semaphorin-6D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC
    TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA
    CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA GGCCAACCAG ATGGTCCACA TGAATGTCCT CATCACCTGT
    GTCTTTGCAG CTTTTGTCTT GGGTGCGTTC ATTGCAGGTG TGGCAGTCTA CTGTTACCGT
    GACATGTTTG TTCGGAAAAA CAGAAAGATC CATAAAGATG CAGAATCTGC CCAGTCATGC
    ACAGACTCCA GTGGAAGTTT TGCCAAGCTG AATGGTCTCT TTGACAGCCC CGTCAAGGAA
    TACCAACAGA ATATTGATTC TCCCAAATTA TATAGTAACC TGCTGACCAG TCGGAAAGAG
    CTGCCACCCA ATGGAGATAC AAAATCCATG GTCATGGACC ATCGAGGCCA ACCTCCTGAG
    TTGGCTGCTC TCCCCACTCC TGAGTCTACG CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCAGAAAA GGCCCATGGC CATGGAGCTT CAAGGAAAGA AACCCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCACATTCC CCACTAAGTC ACGGGCATAT CCCCAGCGCC
    ATTGTTCTTC CTAATGCTAC CCATGACTAC AACACTTCTT TCTCAAACTC CAATGCTCAC
    AAAGCTGAAA AGAAGCTTCA AAACATTGAC CATCCTCTTA CAAAGTCATC CAGTAAAAGA
    GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCATG GGAGACATCC AGATGGCCCA CCAGACCCTA
    ATGCTGGATC CCGTGGGACC TATGTCTGAA GTCCCGCCCA AGGTCCCTAA CCGCGAGGCA
    TCTCTCTACT CTCCTCCCTC GACTCTTCCC AGAAATAGCC CAACCAAGCG AGTGGATGTT
    CCCACCACTC CTGGAGTTCC GATGACTTCT TTGGAAAGAC AGAGGGGTTA CCATAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCGGCTATG CCTAAAAACT TAAGTTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCTAG TATGAACCGT GGAGGGTACG TGCCCACCCC TGCAGGGCCA
    AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGCTCACC TACAGCCTTC CCTCTCCAGA
    CAGAGCAGCT ACACCAGTAA TGGCACCCTT CCCCGGACGG GACTAAAGAG GACACCGTCC
    TTAAAACCTG ACGTGCCGCC AAAGCCTTCA TTTGTTCCTC AAACCACCTC CGTCAGACCA
    CTGAACAAAT ACACTTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853304.1
    CDS939..4199
    Translation

    Target ORF information:

    RefSeq Version XM_024997536.1
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 6D (SEMA6D), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997536.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC
    TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA
    CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTATCCCAAA GGGTGTACGG
    TGGGAAGTCC AGTCTGGAGA GGCCAACCAG ATGGTCCACA TGAATGTCCT CATCACCTGT
    GTCTTTGCAG CTTTTGTCTT GGGTGCGTTC ATTGCAGGTG TGGCAGTCTA CTGTTACCGT
    GACATGTTTG TTCGGAAAAA CAGAAAGATC CATAAAGATG CAGAATCTGC CCAGTCATGC
    ACAGACTCCA GTGGAAGTTT TGCCAAGCTG AATGGTCTCT TTGACAGCCC CGTCAAGGAA
    TACCAACAGA ATATTGATTC TCCCAAATTA TATAGTAACC TGCTGACCAG TCGGAAAGAG
    CTGCCACCCA ATGGAGATAC AAAATCCATG GTCATGGACC ATCGAGGCCA ACCTCCTGAG
    TTGGCTGCTC TCCCCACTCC TGAGTCTACG CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCAGAAAA GGCCCATGGC CATGGAGCTT CAAGGAAAGA AACCCCCCAG
    TTTTTTCCTT CTAGTCCTCC ACCACATTCC CCACTAAGTC ACGGGCATAT CCCCAGCGCC
    ATTGTTCTTC CTAATGCTAC CCATGACTAC AACACTTCTT TCTCAAACTC CAATGCTCAC
    AAAGCTGAAA AGAAGCTTCA AAACATTGAC CATCCTCTTA CAAAGTCATC CAGTAAAAGA
    GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATCTAAAT
    GACCCAAACA GTAACCCCAA AGCCATCATG GGAGACATCC AGATGGCCCA CCAGACCCTA
    ATGCTGGATC CCGTGGGACC TATGTCTGAA GTCCCGCCCA AGGTCCCTAA CCGCGAGGCA
    TCTCTCTACT CTCCTCCCTC GACTCTTCCC AGAAATAGCC CAACCAAGCG AGTGGATGTT
    CCCACCACTC CTGGAGTTCC GATGACTTCT TTGGAAAGAC AGAGGGGTTA CCATAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCGGCTATG CCTAAAAACT TAAGTTCACC AAATGGTGTT
    TTGTTATCTA GACAGCCTAG TATGAACCGT GGAGGGTACG TGCCCACCCC TGCAGGGCCA
    AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGCTCACC TACAGCCTTC CCTCTCCAGA
    CAGAGCAGCT ACACCAGTAA TGGCACCCTT CCCCGGACGG GACTAAAGAG GACACCGTCC
    TTAAAACCTG ACGTGCCGCC AAAGCCTTCA TTTGTTCCTC AAACCACCTC CGTCAGACCA
    CTGAACAAAT ACACTTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49889
    Clone ID Related Accession (Same CDS sequence) XM_010809340.3 , XM_010809340.2
    Accession Version XM_010809340.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product semaphorin-6D isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809340.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC
    TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA
    CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTGTACGGTG GGAAGTCCAG
    TCTGGAGAGG CCAACCAGAT GGTCCACATG AATGTCCTCA TCACCTGTGT CTTTGCAGCT
    TTTGTCTTGG GTGCGTTCAT TGCAGGTGTG GCAGTCTACT GTTACCGTGA CATGTTTGTT
    CGGAAAAACA GAAAGATCCA TAAAGATGCA GAATCTGCCC AGTCATGCAC AGACTCCAGT
    GGAAGTTTTG CCAAGCTGAA TGGTCTCTTT GACAGCCCCG TCAAGGAATA CCAACAGAAT
    ATTGATTCTC CCAAATTATA TAGTAACCTG CTGACCAGTC GGAAAGAGCT GCCACCCAAT
    GGAGATACAA AATCCATGGT CATGGACCAT CGAGGCCAAC CTCCTGAGTT GGCTGCTCTC
    CCCACTCCTG AGTCTACGCC TGTGCTTCAC CAGAAGACCC TGCAGGCCAT GAAGAGCCAC
    TCAGAAAAGG CCCATGGCCA TGGAGCTTCA AGGAAAGAAA CCCCCCAGTT TTTTCCTTCT
    AGTCCTCCAC CACATTCCCC ACTAAGTCAC GGGCATATCC CCAGCGCCAT TGTTCTTCCT
    AATGCTACCC ATGACTACAA CACTTCTTTC TCAAACTCCA ATGCTCACAA AGCTGAAAAG
    AAGCTTCAAA ACATTGACCA TCCTCTTACA AAGTCATCCA GTAAAAGAGA TCACCGGCGT
    TCTGTGGATT CCAGAAATAC CCTCAATGAT CTCCTGAAGC ATCTAAATGA CCCAAACAGT
    AACCCCAAAG CCATCATGGG AGACATCCAG ATGGCCCACC AGACCCTAAT GCTGGATCCC
    GTGGGACCTA TGTCTGAAGT CCCGCCCAAG GTCCCTAACC GCGAGGCATC TCTCTACTCT
    CCTCCCTCGA CTCTTCCCAG AAATAGCCCA ACCAAGCGAG TGGATGTTCC CACCACTCCT
    GGAGTTCCGA TGACTTCTTT GGAAAGACAG AGGGGTTACC ATAAAAATTC CTCCCAGAGG
    CACTCTATAT CGGCTATGCC TAAAAACTTA AGTTCACCAA ATGGTGTTTT GTTATCTAGA
    CAGCCTAGTA TGAACCGTGG AGGGTACGTG CCCACCCCTG CAGGGCCAAA GGTGGACTAT
    ATTCAGGGAG CACCAGTGAG TGCTCACCTA CAGCCTTCCC TCTCCAGACA GAGCAGCTAC
    ACCAGTAATG GCACCCTTCC CCGGACGGGA CTAAAGAGGA CACCGTCCTT AAAACCTGAC
    GTGCCGCCAA AGCCTTCATT TGTTCCTCAA ACCACCTCCG TCAGACCACT GAACAAATAC
    ACTTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807642.1
    CDS180..3428
    Translation

    Target ORF information:

    RefSeq Version XM_010809340.3
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 6D (SEMA6D), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809340.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC
    TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA
    CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTGTACGGTG GGAAGTCCAG
    TCTGGAGAGG CCAACCAGAT GGTCCACATG AATGTCCTCA TCACCTGTGT CTTTGCAGCT
    TTTGTCTTGG GTGCGTTCAT TGCAGGTGTG GCAGTCTACT GTTACCGTGA CATGTTTGTT
    CGGAAAAACA GAAAGATCCA TAAAGATGCA GAATCTGCCC AGTCATGCAC AGACTCCAGT
    GGAAGTTTTG CCAAGCTGAA TGGTCTCTTT GACAGCCCCG TCAAGGAATA CCAACAGAAT
    ATTGATTCTC CCAAATTATA TAGTAACCTG CTGACCAGTC GGAAAGAGCT GCCACCCAAT
    GGAGATACAA AATCCATGGT CATGGACCAT CGAGGCCAAC CTCCTGAGTT GGCTGCTCTC
    CCCACTCCTG AGTCTACGCC TGTGCTTCAC CAGAAGACCC TGCAGGCCAT GAAGAGCCAC
    TCAGAAAAGG CCCATGGCCA TGGAGCTTCA AGGAAAGAAA CCCCCCAGTT TTTTCCTTCT
    AGTCCTCCAC CACATTCCCC ACTAAGTCAC GGGCATATCC CCAGCGCCAT TGTTCTTCCT
    AATGCTACCC ATGACTACAA CACTTCTTTC TCAAACTCCA ATGCTCACAA AGCTGAAAAG
    AAGCTTCAAA ACATTGACCA TCCTCTTACA AAGTCATCCA GTAAAAGAGA TCACCGGCGT
    TCTGTGGATT CCAGAAATAC CCTCAATGAT CTCCTGAAGC ATCTAAATGA CCCAAACAGT
    AACCCCAAAG CCATCATGGG AGACATCCAG ATGGCCCACC AGACCCTAAT GCTGGATCCC
    GTGGGACCTA TGTCTGAAGT CCCGCCCAAG GTCCCTAACC GCGAGGCATC TCTCTACTCT
    CCTCCCTCGA CTCTTCCCAG AAATAGCCCA ACCAAGCGAG TGGATGTTCC CACCACTCCT
    GGAGTTCCGA TGACTTCTTT GGAAAGACAG AGGGGTTACC ATAAAAATTC CTCCCAGAGG
    CACTCTATAT CGGCTATGCC TAAAAACTTA AGTTCACCAA ATGGTGTTTT GTTATCTAGA
    CAGCCTAGTA TGAACCGTGG AGGGTACGTG CCCACCCCTG CAGGGCCAAA GGTGGACTAT
    ATTCAGGGAG CACCAGTGAG TGCTCACCTA CAGCCTTCCC TCTCCAGACA GAGCAGCTAC
    ACCAGTAATG GCACCCTTCC CCGGACGGGA CTAAAGAGGA CACCGTCCTT AAAACCTGAC
    GTGCCGCCAA AGCCTTCATT TGTTCCTCAA ACCACCTCCG TCAGACCACT GAACAAATAC
    ACTTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49889
    Clone ID Related Accession (Same CDS sequence) XM_010809340.3 , XM_010809340.2
    Accession Version XM_010809340.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product semaphorin-6D isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809340.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC
    TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA
    CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTGTACGGTG GGAAGTCCAG
    TCTGGAGAGG CCAACCAGAT GGTCCACATG AATGTCCTCA TCACCTGTGT CTTTGCAGCT
    TTTGTCTTGG GTGCGTTCAT TGCAGGTGTG GCAGTCTACT GTTACCGTGA CATGTTTGTT
    CGGAAAAACA GAAAGATCCA TAAAGATGCA GAATCTGCCC AGTCATGCAC AGACTCCAGT
    GGAAGTTTTG CCAAGCTGAA TGGTCTCTTT GACAGCCCCG TCAAGGAATA CCAACAGAAT
    ATTGATTCTC CCAAATTATA TAGTAACCTG CTGACCAGTC GGAAAGAGCT GCCACCCAAT
    GGAGATACAA AATCCATGGT CATGGACCAT CGAGGCCAAC CTCCTGAGTT GGCTGCTCTC
    CCCACTCCTG AGTCTACGCC TGTGCTTCAC CAGAAGACCC TGCAGGCCAT GAAGAGCCAC
    TCAGAAAAGG CCCATGGCCA TGGAGCTTCA AGGAAAGAAA CCCCCCAGTT TTTTCCTTCT
    AGTCCTCCAC CACATTCCCC ACTAAGTCAC GGGCATATCC CCAGCGCCAT TGTTCTTCCT
    AATGCTACCC ATGACTACAA CACTTCTTTC TCAAACTCCA ATGCTCACAA AGCTGAAAAG
    AAGCTTCAAA ACATTGACCA TCCTCTTACA AAGTCATCCA GTAAAAGAGA TCACCGGCGT
    TCTGTGGATT CCAGAAATAC CCTCAATGAT CTCCTGAAGC ATCTAAATGA CCCAAACAGT
    AACCCCAAAG CCATCATGGG AGACATCCAG ATGGCCCACC AGACCCTAAT GCTGGATCCC
    GTGGGACCTA TGTCTGAAGT CCCGCCCAAG GTCCCTAACC GCGAGGCATC TCTCTACTCT
    CCTCCCTCGA CTCTTCCCAG AAATAGCCCA ACCAAGCGAG TGGATGTTCC CACCACTCCT
    GGAGTTCCGA TGACTTCTTT GGAAAGACAG AGGGGTTACC ATAAAAATTC CTCCCAGAGG
    CACTCTATAT CGGCTATGCC TAAAAACTTA AGTTCACCAA ATGGTGTTTT GTTATCTAGA
    CAGCCTAGTA TGAACCGTGG AGGGTACGTG CCCACCCCTG CAGGGCCAAA GGTGGACTAT
    ATTCAGGGAG CACCAGTGAG TGCTCACCTA CAGCCTTCCC TCTCCAGACA GAGCAGCTAC
    ACCAGTAATG GCACCCTTCC CCGGACGGGA CTAAAGAGGA CACCGTCCTT AAAACCTGAC
    GTGCCGCCAA AGCCTTCATT TGTTCCTCAA ACCACCTCCG TCAGACCACT GAACAAATAC
    ACTTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807642.1
    CDS345..3593
    Translation

    Target ORF information:

    RefSeq Version XM_010809340.2
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 6D (SEMA6D), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809340.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC
    TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA
    CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTGTACGGTG GGAAGTCCAG
    TCTGGAGAGG CCAACCAGAT GGTCCACATG AATGTCCTCA TCACCTGTGT CTTTGCAGCT
    TTTGTCTTGG GTGCGTTCAT TGCAGGTGTG GCAGTCTACT GTTACCGTGA CATGTTTGTT
    CGGAAAAACA GAAAGATCCA TAAAGATGCA GAATCTGCCC AGTCATGCAC AGACTCCAGT
    GGAAGTTTTG CCAAGCTGAA TGGTCTCTTT GACAGCCCCG TCAAGGAATA CCAACAGAAT
    ATTGATTCTC CCAAATTATA TAGTAACCTG CTGACCAGTC GGAAAGAGCT GCCACCCAAT
    GGAGATACAA AATCCATGGT CATGGACCAT CGAGGCCAAC CTCCTGAGTT GGCTGCTCTC
    CCCACTCCTG AGTCTACGCC TGTGCTTCAC CAGAAGACCC TGCAGGCCAT GAAGAGCCAC
    TCAGAAAAGG CCCATGGCCA TGGAGCTTCA AGGAAAGAAA CCCCCCAGTT TTTTCCTTCT
    AGTCCTCCAC CACATTCCCC ACTAAGTCAC GGGCATATCC CCAGCGCCAT TGTTCTTCCT
    AATGCTACCC ATGACTACAA CACTTCTTTC TCAAACTCCA ATGCTCACAA AGCTGAAAAG
    AAGCTTCAAA ACATTGACCA TCCTCTTACA AAGTCATCCA GTAAAAGAGA TCACCGGCGT
    TCTGTGGATT CCAGAAATAC CCTCAATGAT CTCCTGAAGC ATCTAAATGA CCCAAACAGT
    AACCCCAAAG CCATCATGGG AGACATCCAG ATGGCCCACC AGACCCTAAT GCTGGATCCC
    GTGGGACCTA TGTCTGAAGT CCCGCCCAAG GTCCCTAACC GCGAGGCATC TCTCTACTCT
    CCTCCCTCGA CTCTTCCCAG AAATAGCCCA ACCAAGCGAG TGGATGTTCC CACCACTCCT
    GGAGTTCCGA TGACTTCTTT GGAAAGACAG AGGGGTTACC ATAAAAATTC CTCCCAGAGG
    CACTCTATAT CGGCTATGCC TAAAAACTTA AGTTCACCAA ATGGTGTTTT GTTATCTAGA
    CAGCCTAGTA TGAACCGTGG AGGGTACGTG CCCACCCCTG CAGGGCCAAA GGTGGACTAT
    ATTCAGGGAG CACCAGTGAG TGCTCACCTA CAGCCTTCCC TCTCCAGACA GAGCAGCTAC
    ACCAGTAATG GCACCCTTCC CCGGACGGGA CTAAAGAGGA CACCGTCCTT AAAACCTGAC
    GTGCCGCCAA AGCCTTCATT TGTTCCTCAA ACCACCTCCG TCAGACCACT GAACAAATAC
    ACTTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49890
    Clone ID Related Accession (Same CDS sequence) XM_010809341.2 , XM_010809341.3
    Accession Version XM_010809341.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3204bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product semaphorin-6D isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809341.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC
    TGCCACGACA TGGAGGTATC TTCATCTTCT GTTACCACAA TGGCAAGTAT CCCAGAAATT
    ACACCTAAAG TGATTGATAC CTGGAGACCT AAACTGACCA GCTCCCGGAA ATTTGTAGTT
    CAAGATGACC CAAACACTTC TGATTTTACT GATCCTTTAT CAGGTATCCC AAAGGGTGTA
    CGGTGGGAAG TCCAGTCTGG AGAGGCCAAC CAGATGGTCC ACATGAATGT CCTCATCACC
    TGTGTCTTTG CAGCTTTTGT CTTGGGTGCG TTCATTGCAG GTGTGGCAGT CTACTGTTAC
    CGTGACATGT TTGTTCGGAA AAACAGAAAG ATCCATAAAG ATGCAGAATC TGCCCAGTCA
    TGCACAGACT CCAGTGGAAG TTTTGCCAAG CTGAATGGTC TCTTTGACAG CCCCGTCAAG
    GAATACCAAC AGAATATTGA TTCTCCCAAA TTATATAGTA ACCTGCTGAC CAGTCGGAAA
    GAGCTGCCAC CCAATGGAGA TACAAAATCC ATGGTCATGG ACCATCGAGG CCAACCTCCT
    GAGTTGGCTG CTCTCCCCAC TCCTGAGTCT ACGCCTGTGC TTCACCAGAA GACCCTGCAG
    GCCATGAAGA GCCACTCAGA AAAGGCCCAT GGCCATGGAG CTTCAAGGAA AGAAACCCCC
    CAGTTTTTTC CTTCTAGTCC TCCACCACAT TCCCCACTAA GTCACGGGCA TATCCCCAGC
    GCCATTGTTC TTCCTAATGC TACCCATGAC TACAACACTT CTTTCTCAAA CTCCAATGCT
    CACAAAGCTG AAAAGAAGCT TCAAAACATT GACCATCCTC TTACAAAGTC ATCCAGTAAA
    AGAGATCACC GGCGTTCTGT GGATTCCAGA AATACCCTCA ATGATCTCCT GAAGCATCTA
    AATGACCCAA ACAGTAACCC CAAAGCCATC ATGGGAGACA TCCAGATGGC CCACCAGACC
    CTAATGCTGG ATCCCGTGGG ACCTATGTCT GAAGTCCCGC CCAAGGTCCC TAACCGCGAG
    GCATCTCTCT ACTCTCCTCC CTCGACTCTT CCCAGAAATA GCCCAACCAA GCGAGTGGAT
    GTTCCCACCA CTCCTGGAGT TCCGATGACT TCTTTGGAAA GACAGAGGGG TTACCATAAA
    AATTCCTCCC AGAGGCACTC TATATCGGCT ATGCCTAAAA ACTTAAGTTC ACCAAATGGT
    GTTTTGTTAT CTAGACAGCC TAGTATGAAC CGTGGAGGGT ACGTGCCCAC CCCTGCAGGG
    CCAAAGGTGG ACTATATTCA GGGAGCACCA GTGAGTGCTC ACCTACAGCC TTCCCTCTCC
    AGACAGAGCA GCTACACCAG TAATGGCACC CTTCCCCGGA CGGGACTAAA GAGGACACCG
    TCCTTAAAAC CTGACGTGCC GCCAAAGCCT TCATTTGTTC CTCAAACCAC CTCCGTCAGA
    CCACTGAACA AATACACTTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807643.1
    CDS345..3548
    Translation

    Target ORF information:

    RefSeq Version XM_010809341.2
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 6D (SEMA6D), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809341.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC
    TGCCACGACA TGGAGGTATC TTCATCTTCT GTTACCACAA TGGCAAGTAT CCCAGAAATT
    ACACCTAAAG TGATTGATAC CTGGAGACCT AAACTGACCA GCTCCCGGAA ATTTGTAGTT
    CAAGATGACC CAAACACTTC TGATTTTACT GATCCTTTAT CAGGTATCCC AAAGGGTGTA
    CGGTGGGAAG TCCAGTCTGG AGAGGCCAAC CAGATGGTCC ACATGAATGT CCTCATCACC
    TGTGTCTTTG CAGCTTTTGT CTTGGGTGCG TTCATTGCAG GTGTGGCAGT CTACTGTTAC
    CGTGACATGT TTGTTCGGAA AAACAGAAAG ATCCATAAAG ATGCAGAATC TGCCCAGTCA
    TGCACAGACT CCAGTGGAAG TTTTGCCAAG CTGAATGGTC TCTTTGACAG CCCCGTCAAG
    GAATACCAAC AGAATATTGA TTCTCCCAAA TTATATAGTA ACCTGCTGAC CAGTCGGAAA
    GAGCTGCCAC CCAATGGAGA TACAAAATCC ATGGTCATGG ACCATCGAGG CCAACCTCCT
    GAGTTGGCTG CTCTCCCCAC TCCTGAGTCT ACGCCTGTGC TTCACCAGAA GACCCTGCAG
    GCCATGAAGA GCCACTCAGA AAAGGCCCAT GGCCATGGAG CTTCAAGGAA AGAAACCCCC
    CAGTTTTTTC CTTCTAGTCC TCCACCACAT TCCCCACTAA GTCACGGGCA TATCCCCAGC
    GCCATTGTTC TTCCTAATGC TACCCATGAC TACAACACTT CTTTCTCAAA CTCCAATGCT
    CACAAAGCTG AAAAGAAGCT TCAAAACATT GACCATCCTC TTACAAAGTC ATCCAGTAAA
    AGAGATCACC GGCGTTCTGT GGATTCCAGA AATACCCTCA ATGATCTCCT GAAGCATCTA
    AATGACCCAA ACAGTAACCC CAAAGCCATC ATGGGAGACA TCCAGATGGC CCACCAGACC
    CTAATGCTGG ATCCCGTGGG ACCTATGTCT GAAGTCCCGC CCAAGGTCCC TAACCGCGAG
    GCATCTCTCT ACTCTCCTCC CTCGACTCTT CCCAGAAATA GCCCAACCAA GCGAGTGGAT
    GTTCCCACCA CTCCTGGAGT TCCGATGACT TCTTTGGAAA GACAGAGGGG TTACCATAAA
    AATTCCTCCC AGAGGCACTC TATATCGGCT ATGCCTAAAA ACTTAAGTTC ACCAAATGGT
    GTTTTGTTAT CTAGACAGCC TAGTATGAAC CGTGGAGGGT ACGTGCCCAC CCCTGCAGGG
    CCAAAGGTGG ACTATATTCA GGGAGCACCA GTGAGTGCTC ACCTACAGCC TTCCCTCTCC
    AGACAGAGCA GCTACACCAG TAATGGCACC CTTCCCCGGA CGGGACTAAA GAGGACACCG
    TCCTTAAAAC CTGACGTGCC GCCAAAGCCT TCATTTGTTC CTCAAACCAC CTCCGTCAGA
    CCACTGAACA AATACACTTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49890
    Clone ID Related Accession (Same CDS sequence) XM_010809341.2 , XM_010809341.3
    Accession Version XM_010809341.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3204bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product semaphorin-6D isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809341.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC
    TGCCACGACA TGGAGGTATC TTCATCTTCT GTTACCACAA TGGCAAGTAT CCCAGAAATT
    ACACCTAAAG TGATTGATAC CTGGAGACCT AAACTGACCA GCTCCCGGAA ATTTGTAGTT
    CAAGATGACC CAAACACTTC TGATTTTACT GATCCTTTAT CAGGTATCCC AAAGGGTGTA
    CGGTGGGAAG TCCAGTCTGG AGAGGCCAAC CAGATGGTCC ACATGAATGT CCTCATCACC
    TGTGTCTTTG CAGCTTTTGT CTTGGGTGCG TTCATTGCAG GTGTGGCAGT CTACTGTTAC
    CGTGACATGT TTGTTCGGAA AAACAGAAAG ATCCATAAAG ATGCAGAATC TGCCCAGTCA
    TGCACAGACT CCAGTGGAAG TTTTGCCAAG CTGAATGGTC TCTTTGACAG CCCCGTCAAG
    GAATACCAAC AGAATATTGA TTCTCCCAAA TTATATAGTA ACCTGCTGAC CAGTCGGAAA
    GAGCTGCCAC CCAATGGAGA TACAAAATCC ATGGTCATGG ACCATCGAGG CCAACCTCCT
    GAGTTGGCTG CTCTCCCCAC TCCTGAGTCT ACGCCTGTGC TTCACCAGAA GACCCTGCAG
    GCCATGAAGA GCCACTCAGA AAAGGCCCAT GGCCATGGAG CTTCAAGGAA AGAAACCCCC
    CAGTTTTTTC CTTCTAGTCC TCCACCACAT TCCCCACTAA GTCACGGGCA TATCCCCAGC
    GCCATTGTTC TTCCTAATGC TACCCATGAC TACAACACTT CTTTCTCAAA CTCCAATGCT
    CACAAAGCTG AAAAGAAGCT TCAAAACATT GACCATCCTC TTACAAAGTC ATCCAGTAAA
    AGAGATCACC GGCGTTCTGT GGATTCCAGA AATACCCTCA ATGATCTCCT GAAGCATCTA
    AATGACCCAA ACAGTAACCC CAAAGCCATC ATGGGAGACA TCCAGATGGC CCACCAGACC
    CTAATGCTGG ATCCCGTGGG ACCTATGTCT GAAGTCCCGC CCAAGGTCCC TAACCGCGAG
    GCATCTCTCT ACTCTCCTCC CTCGACTCTT CCCAGAAATA GCCCAACCAA GCGAGTGGAT
    GTTCCCACCA CTCCTGGAGT TCCGATGACT TCTTTGGAAA GACAGAGGGG TTACCATAAA
    AATTCCTCCC AGAGGCACTC TATATCGGCT ATGCCTAAAA ACTTAAGTTC ACCAAATGGT
    GTTTTGTTAT CTAGACAGCC TAGTATGAAC CGTGGAGGGT ACGTGCCCAC CCCTGCAGGG
    CCAAAGGTGG ACTATATTCA GGGAGCACCA GTGAGTGCTC ACCTACAGCC TTCCCTCTCC
    AGACAGAGCA GCTACACCAG TAATGGCACC CTTCCCCGGA CGGGACTAAA GAGGACACCG
    TCCTTAAAAC CTGACGTGCC GCCAAAGCCT TCATTTGTTC CTCAAACCAC CTCCGTCAGA
    CCACTGAACA AATACACTTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807643.1
    CDS180..3383
    Translation

    Target ORF information:

    RefSeq Version XM_010809341.3
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 6D (SEMA6D), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809341.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC
    TGCCACGACA TGGAGGTATC TTCATCTTCT GTTACCACAA TGGCAAGTAT CCCAGAAATT
    ACACCTAAAG TGATTGATAC CTGGAGACCT AAACTGACCA GCTCCCGGAA ATTTGTAGTT
    CAAGATGACC CAAACACTTC TGATTTTACT GATCCTTTAT CAGGTATCCC AAAGGGTGTA
    CGGTGGGAAG TCCAGTCTGG AGAGGCCAAC CAGATGGTCC ACATGAATGT CCTCATCACC
    TGTGTCTTTG CAGCTTTTGT CTTGGGTGCG TTCATTGCAG GTGTGGCAGT CTACTGTTAC
    CGTGACATGT TTGTTCGGAA AAACAGAAAG ATCCATAAAG ATGCAGAATC TGCCCAGTCA
    TGCACAGACT CCAGTGGAAG TTTTGCCAAG CTGAATGGTC TCTTTGACAG CCCCGTCAAG
    GAATACCAAC AGAATATTGA TTCTCCCAAA TTATATAGTA ACCTGCTGAC CAGTCGGAAA
    GAGCTGCCAC CCAATGGAGA TACAAAATCC ATGGTCATGG ACCATCGAGG CCAACCTCCT
    GAGTTGGCTG CTCTCCCCAC TCCTGAGTCT ACGCCTGTGC TTCACCAGAA GACCCTGCAG
    GCCATGAAGA GCCACTCAGA AAAGGCCCAT GGCCATGGAG CTTCAAGGAA AGAAACCCCC
    CAGTTTTTTC CTTCTAGTCC TCCACCACAT TCCCCACTAA GTCACGGGCA TATCCCCAGC
    GCCATTGTTC TTCCTAATGC TACCCATGAC TACAACACTT CTTTCTCAAA CTCCAATGCT
    CACAAAGCTG AAAAGAAGCT TCAAAACATT GACCATCCTC TTACAAAGTC ATCCAGTAAA
    AGAGATCACC GGCGTTCTGT GGATTCCAGA AATACCCTCA ATGATCTCCT GAAGCATCTA
    AATGACCCAA ACAGTAACCC CAAAGCCATC ATGGGAGACA TCCAGATGGC CCACCAGACC
    CTAATGCTGG ATCCCGTGGG ACCTATGTCT GAAGTCCCGC CCAAGGTCCC TAACCGCGAG
    GCATCTCTCT ACTCTCCTCC CTCGACTCTT CCCAGAAATA GCCCAACCAA GCGAGTGGAT
    GTTCCCACCA CTCCTGGAGT TCCGATGACT TCTTTGGAAA GACAGAGGGG TTACCATAAA
    AATTCCTCCC AGAGGCACTC TATATCGGCT ATGCCTAAAA ACTTAAGTTC ACCAAATGGT
    GTTTTGTTAT CTAGACAGCC TAGTATGAAC CGTGGAGGGT ACGTGCCCAC CCCTGCAGGG
    CCAAAGGTGG ACTATATTCA GGGAGCACCA GTGAGTGCTC ACCTACAGCC TTCCCTCTCC
    AGACAGAGCA GCTACACCAG TAATGGCACC CTTCCCCGGA CGGGACTAAA GAGGACACCG
    TCCTTAAAAC CTGACGTGCC GCCAAAGCCT TCATTTGTTC CTCAAACCAC CTCCGTCAGA
    CCACTGAACA AATACACTTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49891
    Clone ID Related Accession (Same CDS sequence) XM_010809342.3 , XM_010809342.2
    Accession Version XM_010809342.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3165bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product semaphorin-6D isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809342.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC
    AACACGGCCC ATCTAGGGGA CTGCCACGAC ATGGAGGTAT CTTCATCTTC TGTTACCACA
    ATGGCAAGTA TCCCAGAAAT TACACCTAAA GTGATTGATA CCTGGAGACC TAAACTGACC
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAACACTT CTGATTTTAC TGATCCTTTA
    TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAGGCCAA CCAGATGGTC
    CACATGAATG TCCTCATCAC CTGTGTCTTT GCAGCTTTTG TCTTGGGTGC GTTCATTGCA
    GGTGTGGCAG TCTACTGTTA CCGTGACATG TTTGTTCGGA AAAACAGAAA GATCCATAAA
    GATGCAGAAT CTGCCCAGTC ATGCACAGAC TCCAGTGGAA GTTTTGCCAA GCTGAATGGT
    CTCTTTGACA GCCCCGTCAA GGAATACCAA CAGAATATTG ATTCTCCCAA ATTATATAGT
    AACCTGCTGA CCAGTCGGAA AGAGCTGCCA CCCAATGGAG ATACAAAATC CATGGTCATG
    GACCATCGAG GCCAACCTCC TGAGTTGGCT GCTCTCCCCA CTCCTGAGTC TACGCCTGTG
    CTTCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCAG AAAAGGCCCA TGGCCATGGA
    GCTTCAAGGA AAGAAACCCC CCAGTTTTTT CCTTCTAGTC CTCCACCACA TTCCCCACTA
    AGTCACGGGC ATATCCCCAG CGCCATTGTT CTTCCTAATG CTACCCATGA CTACAACACT
    TCTTTCTCAA ACTCCAATGC TCACAAAGCT GAAAAGAAGC TTCAAAACAT TGACCATCCT
    CTTACAAAGT CATCCAGTAA AAGAGATCAC CGGCGTTCTG TGGATTCCAG AAATACCCTC
    AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CATGGGAGAC
    ATCCAGATGG CCCACCAGAC CCTAATGCTG GATCCCGTGG GACCTATGTC TGAAGTCCCG
    CCCAAGGTCC CTAACCGCGA GGCATCTCTC TACTCTCCTC CCTCGACTCT TCCCAGAAAT
    AGCCCAACCA AGCGAGTGGA TGTTCCCACC ACTCCTGGAG TTCCGATGAC TTCTTTGGAA
    AGACAGAGGG GTTACCATAA AAATTCCTCC CAGAGGCACT CTATATCGGC TATGCCTAAA
    AACTTAAGTT CACCAAATGG TGTTTTGTTA TCTAGACAGC CTAGTATGAA CCGTGGAGGG
    TACGTGCCCA CCCCTGCAGG GCCAAAGGTG GACTATATTC AGGGAGCACC AGTGAGTGCT
    CACCTACAGC CTTCCCTCTC CAGACAGAGC AGCTACACCA GTAATGGCAC CCTTCCCCGG
    ACGGGACTAA AGAGGACACC GTCCTTAAAA CCTGACGTGC CGCCAAAGCC TTCATTTGTT
    CCTCAAACCA CCTCCGTCAG ACCACTGAAC AAATACACTT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807644.1
    CDS180..3344
    Translation

    Target ORF information:

    RefSeq Version XM_010809342.3
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 6D (SEMA6D), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809342.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC
    AACACGGCCC ATCTAGGGGA CTGCCACGAC ATGGAGGTAT CTTCATCTTC TGTTACCACA
    ATGGCAAGTA TCCCAGAAAT TACACCTAAA GTGATTGATA CCTGGAGACC TAAACTGACC
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAACACTT CTGATTTTAC TGATCCTTTA
    TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAGGCCAA CCAGATGGTC
    CACATGAATG TCCTCATCAC CTGTGTCTTT GCAGCTTTTG TCTTGGGTGC GTTCATTGCA
    GGTGTGGCAG TCTACTGTTA CCGTGACATG TTTGTTCGGA AAAACAGAAA GATCCATAAA
    GATGCAGAAT CTGCCCAGTC ATGCACAGAC TCCAGTGGAA GTTTTGCCAA GCTGAATGGT
    CTCTTTGACA GCCCCGTCAA GGAATACCAA CAGAATATTG ATTCTCCCAA ATTATATAGT
    AACCTGCTGA CCAGTCGGAA AGAGCTGCCA CCCAATGGAG ATACAAAATC CATGGTCATG
    GACCATCGAG GCCAACCTCC TGAGTTGGCT GCTCTCCCCA CTCCTGAGTC TACGCCTGTG
    CTTCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCAG AAAAGGCCCA TGGCCATGGA
    GCTTCAAGGA AAGAAACCCC CCAGTTTTTT CCTTCTAGTC CTCCACCACA TTCCCCACTA
    AGTCACGGGC ATATCCCCAG CGCCATTGTT CTTCCTAATG CTACCCATGA CTACAACACT
    TCTTTCTCAA ACTCCAATGC TCACAAAGCT GAAAAGAAGC TTCAAAACAT TGACCATCCT
    CTTACAAAGT CATCCAGTAA AAGAGATCAC CGGCGTTCTG TGGATTCCAG AAATACCCTC
    AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CATGGGAGAC
    ATCCAGATGG CCCACCAGAC CCTAATGCTG GATCCCGTGG GACCTATGTC TGAAGTCCCG
    CCCAAGGTCC CTAACCGCGA GGCATCTCTC TACTCTCCTC CCTCGACTCT TCCCAGAAAT
    AGCCCAACCA AGCGAGTGGA TGTTCCCACC ACTCCTGGAG TTCCGATGAC TTCTTTGGAA
    AGACAGAGGG GTTACCATAA AAATTCCTCC CAGAGGCACT CTATATCGGC TATGCCTAAA
    AACTTAAGTT CACCAAATGG TGTTTTGTTA TCTAGACAGC CTAGTATGAA CCGTGGAGGG
    TACGTGCCCA CCCCTGCAGG GCCAAAGGTG GACTATATTC AGGGAGCACC AGTGAGTGCT
    CACCTACAGC CTTCCCTCTC CAGACAGAGC AGCTACACCA GTAATGGCAC CCTTCCCCGG
    ACGGGACTAA AGAGGACACC GTCCTTAAAA CCTGACGTGC CGCCAAAGCC TTCATTTGTT
    CCTCAAACCA CCTCCGTCAG ACCACTGAAC AAATACACTT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49891
    Clone ID Related Accession (Same CDS sequence) XM_010809342.3 , XM_010809342.2
    Accession Version XM_010809342.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3165bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product semaphorin-6D isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809342.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC
    AACACGGCCC ATCTAGGGGA CTGCCACGAC ATGGAGGTAT CTTCATCTTC TGTTACCACA
    ATGGCAAGTA TCCCAGAAAT TACACCTAAA GTGATTGATA CCTGGAGACC TAAACTGACC
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAACACTT CTGATTTTAC TGATCCTTTA
    TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAGGCCAA CCAGATGGTC
    CACATGAATG TCCTCATCAC CTGTGTCTTT GCAGCTTTTG TCTTGGGTGC GTTCATTGCA
    GGTGTGGCAG TCTACTGTTA CCGTGACATG TTTGTTCGGA AAAACAGAAA GATCCATAAA
    GATGCAGAAT CTGCCCAGTC ATGCACAGAC TCCAGTGGAA GTTTTGCCAA GCTGAATGGT
    CTCTTTGACA GCCCCGTCAA GGAATACCAA CAGAATATTG ATTCTCCCAA ATTATATAGT
    AACCTGCTGA CCAGTCGGAA AGAGCTGCCA CCCAATGGAG ATACAAAATC CATGGTCATG
    GACCATCGAG GCCAACCTCC TGAGTTGGCT GCTCTCCCCA CTCCTGAGTC TACGCCTGTG
    CTTCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCAG AAAAGGCCCA TGGCCATGGA
    GCTTCAAGGA AAGAAACCCC CCAGTTTTTT CCTTCTAGTC CTCCACCACA TTCCCCACTA
    AGTCACGGGC ATATCCCCAG CGCCATTGTT CTTCCTAATG CTACCCATGA CTACAACACT
    TCTTTCTCAA ACTCCAATGC TCACAAAGCT GAAAAGAAGC TTCAAAACAT TGACCATCCT
    CTTACAAAGT CATCCAGTAA AAGAGATCAC CGGCGTTCTG TGGATTCCAG AAATACCCTC
    AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CATGGGAGAC
    ATCCAGATGG CCCACCAGAC CCTAATGCTG GATCCCGTGG GACCTATGTC TGAAGTCCCG
    CCCAAGGTCC CTAACCGCGA GGCATCTCTC TACTCTCCTC CCTCGACTCT TCCCAGAAAT
    AGCCCAACCA AGCGAGTGGA TGTTCCCACC ACTCCTGGAG TTCCGATGAC TTCTTTGGAA
    AGACAGAGGG GTTACCATAA AAATTCCTCC CAGAGGCACT CTATATCGGC TATGCCTAAA
    AACTTAAGTT CACCAAATGG TGTTTTGTTA TCTAGACAGC CTAGTATGAA CCGTGGAGGG
    TACGTGCCCA CCCCTGCAGG GCCAAAGGTG GACTATATTC AGGGAGCACC AGTGAGTGCT
    CACCTACAGC CTTCCCTCTC CAGACAGAGC AGCTACACCA GTAATGGCAC CCTTCCCCGG
    ACGGGACTAA AGAGGACACC GTCCTTAAAA CCTGACGTGC CGCCAAAGCC TTCATTTGTT
    CCTCAAACCA CCTCCGTCAG ACCACTGAAC AAATACACTT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807644.1
    CDS345..3509
    Translation

    Target ORF information:

    RefSeq Version XM_010809342.2
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 6D (SEMA6D), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809342.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG
    TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG
    CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA
    AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT
    CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC
    AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC
    AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA
    ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC
    TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT
    GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG
    AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA
    AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT
    GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA
    TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC
    TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC
    AACACGGCCC ATCTAGGGGA CTGCCACGAC ATGGAGGTAT CTTCATCTTC TGTTACCACA
    ATGGCAAGTA TCCCAGAAAT TACACCTAAA GTGATTGATA CCTGGAGACC TAAACTGACC
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAACACTT CTGATTTTAC TGATCCTTTA
    TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAGGCCAA CCAGATGGTC
    CACATGAATG TCCTCATCAC CTGTGTCTTT GCAGCTTTTG TCTTGGGTGC GTTCATTGCA
    GGTGTGGCAG TCTACTGTTA CCGTGACATG TTTGTTCGGA AAAACAGAAA GATCCATAAA
    GATGCAGAAT CTGCCCAGTC ATGCACAGAC TCCAGTGGAA GTTTTGCCAA GCTGAATGGT
    CTCTTTGACA GCCCCGTCAA GGAATACCAA CAGAATATTG ATTCTCCCAA ATTATATAGT
    AACCTGCTGA CCAGTCGGAA AGAGCTGCCA CCCAATGGAG ATACAAAATC CATGGTCATG
    GACCATCGAG GCCAACCTCC TGAGTTGGCT GCTCTCCCCA CTCCTGAGTC TACGCCTGTG
    CTTCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCAG AAAAGGCCCA TGGCCATGGA
    GCTTCAAGGA AAGAAACCCC CCAGTTTTTT CCTTCTAGTC CTCCACCACA TTCCCCACTA
    AGTCACGGGC ATATCCCCAG CGCCATTGTT CTTCCTAATG CTACCCATGA CTACAACACT
    TCTTTCTCAA ACTCCAATGC TCACAAAGCT GAAAAGAAGC TTCAAAACAT TGACCATCCT
    CTTACAAAGT CATCCAGTAA AAGAGATCAC CGGCGTTCTG TGGATTCCAG AAATACCCTC
    AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CATGGGAGAC
    ATCCAGATGG CCCACCAGAC CCTAATGCTG GATCCCGTGG GACCTATGTC TGAAGTCCCG
    CCCAAGGTCC CTAACCGCGA GGCATCTCTC TACTCTCCTC CCTCGACTCT TCCCAGAAAT
    AGCCCAACCA AGCGAGTGGA TGTTCCCACC ACTCCTGGAG TTCCGATGAC TTCTTTGGAA
    AGACAGAGGG GTTACCATAA AAATTCCTCC CAGAGGCACT CTATATCGGC TATGCCTAAA
    AACTTAAGTT CACCAAATGG TGTTTTGTTA TCTAGACAGC CTAGTATGAA CCGTGGAGGG
    TACGTGCCCA CCCCTGCAGG GCCAAAGGTG GACTATATTC AGGGAGCACC AGTGAGTGCT
    CACCTACAGC CTTCCCTCTC CAGACAGAGC AGCTACACCA GTAATGGCAC CCTTCCCCGG
    ACGGGACTAA AGAGGACACC GTCCTTAAAA CCTGACGTGC CGCCAAAGCC TTCATTTGTT
    CCTCAAACCA CCTCCGTCAG ACCACTGAAC AAATACACTT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.