SEMA6D gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005211812.3	XP_005211869.1	semaphorin-6D isoform X5
XM_010809340.3	XP_010807642.1	semaphorin-6D isoform X2
XM_005211814.4	XP_005211871.1	semaphorin-6D isoform X9
XM_010809341.2	XP_010807643.1	semaphorin-6D isoform X3
XM_005211814.3	XP_005211871.1	semaphorin-6D isoform X7
XM_005211815.3	XP_005211872.1	semaphorin-6D isoform X8
XM_024997535.1	XP_024853303.1	semaphorin-6D isoform X1
XM_010809342.3	XP_010807644.1	semaphorin-6D isoform X5
XM_010809340.2	XP_010807642.1	semaphorin-6D isoform X2
XM_024997534.1	XP_024853302.1	semaphorin-6D isoform X1
XM_010809341.3	XP_010807643.1	semaphorin-6D isoform X4
XM_005211815.4	XP_005211872.1	semaphorin-6D isoform X10
XM_010809342.2	XP_010807644.1	semaphorin-6D isoform X4
NM_001191133.1	NP_001178062.1	semaphorin-6D precursor
XM_010809338.3	XP_010807640.1	semaphorin-6D isoform X1
XM_024997538.1	XP_024853306.1	semaphorin-6D isoform X6
XM_010809338.2	XP_010807640.1	semaphorin-6D isoform X1
XM_005211813.3	XP_005211870.1	semaphorin-6D isoform X6
XM_024997536.1	XP_024853304.1	semaphorin-6D isoform X1
XM_024997537.1	XP_024853305.1	semaphorin-6D isoform X3
XM_005211812.4	XP_005211869.1	semaphorin-6D isoform X7
XM_005211813.4	XP_005211870.1	semaphorin-6D isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa23347	XM_005211815.4 Latest version!	Bos taurus semaphorin 6D (SEMA6D), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa219557	XM_024997537.1 Latest version!	Bos taurus semaphorin 6D (SEMA6D), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa219558	XM_024997538.1 Latest version!	Bos taurus semaphorin 6D (SEMA6D), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa40864	NM_001191133.1 Latest version!	Bos taurus semaphorin 6D (SEMA6D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa23344	XM_005211812.4 Latest version!	Bos taurus semaphorin 6D (SEMA6D), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa23345	XM_005211813.4 Latest version!	Bos taurus semaphorin 6D (SEMA6D), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa23346	XM_005211814.4 Latest version!	Bos taurus semaphorin 6D (SEMA6D), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa49888	XM_024997536.1 Latest version!	Bos taurus semaphorin 6D (SEMA6D), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa49888	XM_024997535.1 Latest version!	Bos taurus semaphorin 6D (SEMA6D), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa49888	XM_010809338.3 Latest version!	Bos taurus semaphorin 6D (SEMA6D), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa49888	XM_024997534.1 Latest version!	Bos taurus semaphorin 6D (SEMA6D), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa49889	XM_010809340.3 Latest version!	Bos taurus semaphorin 6D (SEMA6D), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa49890	XM_010809341.3 Latest version!	Bos taurus semaphorin 6D (SEMA6D), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa49891	XM_010809342.3 Latest version!	Bos taurus semaphorin 6D (SEMA6D), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OBa23346	XM_005211814.3	Bos taurus semaphorin 6D (SEMA6D), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa49889	XM_010809340.2	Bos taurus semaphorin 6D (SEMA6D), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa49890	XM_010809341.2	Bos taurus semaphorin 6D (SEMA6D), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa23347	XM_005211815.3	Bos taurus semaphorin 6D (SEMA6D), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa49891	XM_010809342.2	Bos taurus semaphorin 6D (SEMA6D), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa49888	XM_010809338.2	Bos taurus semaphorin 6D (SEMA6D), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa23344	XM_005211812.3	Bos taurus semaphorin 6D (SEMA6D), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa23345	XM_005211813.3	Bos taurus semaphorin 6D (SEMA6D), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

SEMA6D ( XM_005211815.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211872.1 Bos taurus semaphorin 6D (SEMA6D), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa23347
Clone ID Related Accession (Same CDS sequence)	XM_005211815.3 , XM_005211815.4
Accession Version	XM_005211815.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	semaphorin-6D isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211815.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC 
AACACGGCCC ATCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAGGCC 
AACCAGATGG TCCACATGAA TGTCCTCATC ACCTGTGTCT TTGCAGCTTT TGTCTTGGGT 
GCGTTCATTG CAGGTGTGGC AGTCTACTGT TACCGTGACA TGTTTGTTCG GAAAAACAGA 
AAGATCCATA AAGATGCAGA ATCTGCCCAG TCATGCACAG ACTCCAGTGG AAGTTTTGCC 
AAGCTGAATG GTCTCTTTGA CAGCCCCGTC AAGGAATACC AACAGAATAT TGATTCTCCC 
AAATTATATA GTAACCTGCT GACCAGTCGG AAAGAGCTGC CACCCAATGG AGATACAAAA 
TCCATGGTCA TGGACCATCG AGGCCAACCT CCTGAGTTGG CTGCTCTCCC CACTCCTGAG 
TCTACGCCTG TGCTTCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC AGAAAAGGCC 
CATGGCCATG GAGCTTCAAG GAAAGAAACC CCCCAGTTTT TTCCTTCTAG TCCTCCACCA 
CATTCCCCAC TAAGTCACGG GCATATCCCC AGCGCCATTG TTCTTCCTAA TGCTACCCAT 
GACTACAACA CTTCTTTCTC AAACTCCAAT GCTCACAAAG CTGAAAAGAA GCTTCAAAAC 
ATTGACCATC CTCTTACAAA GTCATCCAGT AAAAGAGATC ACCGGCGTTC TGTGGATTCC 
AGAAATACCC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC 
ATCATGGGAG ACATCCAGAT GGCCCACCAG ACCCTAATGC TGGATCCCGT GGGACCTATG 
TCTGAAGTCC CGCCCAAGGT CCCTAACCGC GAGGCATCTC TCTACTCTCC TCCCTCGACT 
CTTCCCAGAA ATAGCCCAAC CAAGCGAGTG GATGTTCCCA CCACTCCTGG AGTTCCGATG 
ACTTCTTTGG AAAGACAGAG GGGTTACCAT AAAAATTCCT CCCAGAGGCA CTCTATATCG 
GCTATGCCTA AAAACTTAAG TTCACCAAAT GGTGTTTTGT TATCTAGACA GCCTAGTATG 
AACCGTGGAG GGTACGTGCC CACCCCTGCA GGGCCAAAGG TGGACTATAT TCAGGGAGCA 
CCAGTGAGTG CTCACCTACA GCCTTCCCTC TCCAGACAGA GCAGCTACAC CAGTAATGGC 
ACCCTTCCCC GGACGGGACT AAAGAGGACA CCGTCCTTAA AACCTGACGT GCCGCCAAAG 
CCTTCATTTG TTCCTCAAAC CACCTCCGTC AGACCACTGA ACAAATACAC TTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211872.1
CDS	345..3341
Translation	MRFFLLCAYMLLLLISQLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPKTEVIPNKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYKLNTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDETLSFIKSHPLMDSAVPPIADEPWFTKTRIRYRLTAIAVDHSAGPHQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNHAKCSAENEEDRKVISLQLDKDHHALYVAFSSCVIRIPLSRCERYGSCKKSCIASRDPYCGWLSQGACGRVSPAMLAGGFEQDTEYGNTAHLGDCHGVRWEVQSGEANQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNGDTKSMVMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHGHGASRKETPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNIDHPLTKSSSKRDHRRSVDSRNTLNDLLKHLNDPNSNPKAIMGDIQMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAMPKNLSSPNGVLLSRQPSMNRGGYVPTPAGPKVDYIQGAPVSAHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_005211815.3
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 6D (SEMA6D), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211815.3

ORF Insert Sequence:

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC 
AACACGGCCC ATCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAGGCC 
AACCAGATGG TCCACATGAA TGTCCTCATC ACCTGTGTCT TTGCAGCTTT TGTCTTGGGT 
GCGTTCATTG CAGGTGTGGC AGTCTACTGT TACCGTGACA TGTTTGTTCG GAAAAACAGA 
AAGATCCATA AAGATGCAGA ATCTGCCCAG TCATGCACAG ACTCCAGTGG AAGTTTTGCC 
AAGCTGAATG GTCTCTTTGA CAGCCCCGTC AAGGAATACC AACAGAATAT TGATTCTCCC 
AAATTATATA GTAACCTGCT GACCAGTCGG AAAGAGCTGC CACCCAATGG AGATACAAAA 
TCCATGGTCA TGGACCATCG AGGCCAACCT CCTGAGTTGG CTGCTCTCCC CACTCCTGAG 
TCTACGCCTG TGCTTCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC AGAAAAGGCC 
CATGGCCATG GAGCTTCAAG GAAAGAAACC CCCCAGTTTT TTCCTTCTAG TCCTCCACCA 
CATTCCCCAC TAAGTCACGG GCATATCCCC AGCGCCATTG TTCTTCCTAA TGCTACCCAT 
GACTACAACA CTTCTTTCTC AAACTCCAAT GCTCACAAAG CTGAAAAGAA GCTTCAAAAC 
ATTGACCATC CTCTTACAAA GTCATCCAGT AAAAGAGATC ACCGGCGTTC TGTGGATTCC 
AGAAATACCC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC 
ATCATGGGAG ACATCCAGAT GGCCCACCAG ACCCTAATGC TGGATCCCGT GGGACCTATG 
TCTGAAGTCC CGCCCAAGGT CCCTAACCGC GAGGCATCTC TCTACTCTCC TCCCTCGACT 
CTTCCCAGAA ATAGCCCAAC CAAGCGAGTG GATGTTCCCA CCACTCCTGG AGTTCCGATG 
ACTTCTTTGG AAAGACAGAG GGGTTACCAT AAAAATTCCT CCCAGAGGCA CTCTATATCG 
GCTATGCCTA AAAACTTAAG TTCACCAAAT GGTGTTTTGT TATCTAGACA GCCTAGTATG 
AACCGTGGAG GGTACGTGCC CACCCCTGCA GGGCCAAAGG TGGACTATAT TCAGGGAGCA 
CCAGTGAGTG CTCACCTACA GCCTTCCCTC TCCAGACAGA GCAGCTACAC CAGTAATGGC 
ACCCTTCCCC GGACGGGACT AAAGAGGACA CCGTCCTTAA AACCTGACGT GCCGCCAAAG 
CCTTCATTTG TTCCTCAAAC CACCTCCGTC AGACCACTGA ACAAATACAC TTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA6D ( XM_005211815.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211872.1 Bos taurus semaphorin 6D (SEMA6D), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBa23347
Clone ID Related Accession (Same CDS sequence)	XM_005211815.3 , XM_005211815.4
Accession Version	XM_005211815.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	semaphorin-6D isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211815.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC 
AACACGGCCC ATCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAGGCC 
AACCAGATGG TCCACATGAA TGTCCTCATC ACCTGTGTCT TTGCAGCTTT TGTCTTGGGT 
GCGTTCATTG CAGGTGTGGC AGTCTACTGT TACCGTGACA TGTTTGTTCG GAAAAACAGA 
AAGATCCATA AAGATGCAGA ATCTGCCCAG TCATGCACAG ACTCCAGTGG AAGTTTTGCC 
AAGCTGAATG GTCTCTTTGA CAGCCCCGTC AAGGAATACC AACAGAATAT TGATTCTCCC 
AAATTATATA GTAACCTGCT GACCAGTCGG AAAGAGCTGC CACCCAATGG AGATACAAAA 
TCCATGGTCA TGGACCATCG AGGCCAACCT CCTGAGTTGG CTGCTCTCCC CACTCCTGAG 
TCTACGCCTG TGCTTCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC AGAAAAGGCC 
CATGGCCATG GAGCTTCAAG GAAAGAAACC CCCCAGTTTT TTCCTTCTAG TCCTCCACCA 
CATTCCCCAC TAAGTCACGG GCATATCCCC AGCGCCATTG TTCTTCCTAA TGCTACCCAT 
GACTACAACA CTTCTTTCTC AAACTCCAAT GCTCACAAAG CTGAAAAGAA GCTTCAAAAC 
ATTGACCATC CTCTTACAAA GTCATCCAGT AAAAGAGATC ACCGGCGTTC TGTGGATTCC 
AGAAATACCC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC 
ATCATGGGAG ACATCCAGAT GGCCCACCAG ACCCTAATGC TGGATCCCGT GGGACCTATG 
TCTGAAGTCC CGCCCAAGGT CCCTAACCGC GAGGCATCTC TCTACTCTCC TCCCTCGACT 
CTTCCCAGAA ATAGCCCAAC CAAGCGAGTG GATGTTCCCA CCACTCCTGG AGTTCCGATG 
ACTTCTTTGG AAAGACAGAG GGGTTACCAT AAAAATTCCT CCCAGAGGCA CTCTATATCG 
GCTATGCCTA AAAACTTAAG TTCACCAAAT GGTGTTTTGT TATCTAGACA GCCTAGTATG 
AACCGTGGAG GGTACGTGCC CACCCCTGCA GGGCCAAAGG TGGACTATAT TCAGGGAGCA 
CCAGTGAGTG CTCACCTACA GCCTTCCCTC TCCAGACAGA GCAGCTACAC CAGTAATGGC 
ACCCTTCCCC GGACGGGACT AAAGAGGACA CCGTCCTTAA AACCTGACGT GCCGCCAAAG 
CCTTCATTTG TTCCTCAAAC CACCTCCGTC AGACCACTGA ACAAATACAC TTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211872.1
CDS	180..3176
Translation	MRFFLLCAYMLLLLISQLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPKTEVIPNKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYKLNTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDETLSFIKSHPLMDSAVPPIADEPWFTKTRIRYRLTAIAVDHSAGPHQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNHAKCSAENEEDRKVISLQLDKDHHALYVAFSSCVIRIPLSRCERYGSCKKSCIASRDPYCGWLSQGACGRVSPAMLAGGFEQDTEYGNTAHLGDCHGVRWEVQSGEANQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNGDTKSMVMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHGHGASRKETPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNIDHPLTKSSSKRDHRRSVDSRNTLNDLLKHLNDPNSNPKAIMGDIQMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAMPKNLSSPNGVLLSRQPSMNRGGYVPTPAGPKVDYIQGAPVSAHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_005211815.4
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 6D (SEMA6D), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211815.4

ORF Insert Sequence:

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC 
AACACGGCCC ATCTAGGGGA CTGCCACGGT GTACGGTGGG AAGTCCAGTC TGGAGAGGCC 
AACCAGATGG TCCACATGAA TGTCCTCATC ACCTGTGTCT TTGCAGCTTT TGTCTTGGGT 
GCGTTCATTG CAGGTGTGGC AGTCTACTGT TACCGTGACA TGTTTGTTCG GAAAAACAGA 
AAGATCCATA AAGATGCAGA ATCTGCCCAG TCATGCACAG ACTCCAGTGG AAGTTTTGCC 
AAGCTGAATG GTCTCTTTGA CAGCCCCGTC AAGGAATACC AACAGAATAT TGATTCTCCC 
AAATTATATA GTAACCTGCT GACCAGTCGG AAAGAGCTGC CACCCAATGG AGATACAAAA 
TCCATGGTCA TGGACCATCG AGGCCAACCT CCTGAGTTGG CTGCTCTCCC CACTCCTGAG 
TCTACGCCTG TGCTTCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC AGAAAAGGCC 
CATGGCCATG GAGCTTCAAG GAAAGAAACC CCCCAGTTTT TTCCTTCTAG TCCTCCACCA 
CATTCCCCAC TAAGTCACGG GCATATCCCC AGCGCCATTG TTCTTCCTAA TGCTACCCAT 
GACTACAACA CTTCTTTCTC AAACTCCAAT GCTCACAAAG CTGAAAAGAA GCTTCAAAAC 
ATTGACCATC CTCTTACAAA GTCATCCAGT AAAAGAGATC ACCGGCGTTC TGTGGATTCC 
AGAAATACCC TCAATGATCT CCTGAAGCAT CTAAATGACC CAAACAGTAA CCCCAAAGCC 
ATCATGGGAG ACATCCAGAT GGCCCACCAG ACCCTAATGC TGGATCCCGT GGGACCTATG 
TCTGAAGTCC CGCCCAAGGT CCCTAACCGC GAGGCATCTC TCTACTCTCC TCCCTCGACT 
CTTCCCAGAA ATAGCCCAAC CAAGCGAGTG GATGTTCCCA CCACTCCTGG AGTTCCGATG 
ACTTCTTTGG AAAGACAGAG GGGTTACCAT AAAAATTCCT CCCAGAGGCA CTCTATATCG 
GCTATGCCTA AAAACTTAAG TTCACCAAAT GGTGTTTTGT TATCTAGACA GCCTAGTATG 
AACCGTGGAG GGTACGTGCC CACCCCTGCA GGGCCAAAGG TGGACTATAT TCAGGGAGCA 
CCAGTGAGTG CTCACCTACA GCCTTCCCTC TCCAGACAGA GCAGCTACAC CAGTAATGGC 
ACCCTTCCCC GGACGGGACT AAAGAGGACA CCGTCCTTAA AACCTGACGT GCCGCCAAAG 
CCTTCATTTG TTCCTCAAAC CACCTCCGTC AGACCACTGA ACAAATACAC TTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA6D ( XM_024997537.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853305.1 Bos taurus semaphorin 6D (SEMA6D), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa219557
Clone ID Related Accession (Same CDS sequence)	XM_024997537.1
Accession Version	XM_024997537.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	semaphorin-6D isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC 
AACACGGCCC ATCTAGGGGA CTGCCACGAA ATTTTGCCTA CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCTT CATCTTCTGT TACCACAATG 
GCAAGTATCC CAGAAATTAC ACCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACCAGC 
TCCCGGAAAT TTGTAGTTCA AGATGACCCA AACACTTCTG ATTTTACTGA TCCTTTATCA 
GGTGTACGGT GGGAAGTCCA GTCTGGAGAG GCCAACCAGA TGGTCCACAT GAATGTCCTC 
ATCACCTGTG TCTTTGCAGC TTTTGTCTTG GGTGCGTTCA TTGCAGGTGT GGCAGTCTAC 
TGTTACCGTG ACATGTTTGT TCGGAAAAAC AGAAAGATCC ATAAAGATGC AGAATCTGCC 
CAGTCATGCA CAGACTCCAG TGGAAGTTTT GCCAAGCTGA ATGGTCTCTT TGACAGCCCC 
GTCAAGGAAT ACCAACAGAA TATTGATTCT CCCAAATTAT ATAGTAACCT GCTGACCAGT 
CGGAAAGAGC TGCCACCCAA TGGAGATACA AAATCCATGG TCATGGACCA TCGAGGCCAA 
CCTCCTGAGT TGGCTGCTCT CCCCACTCCT GAGTCTACGC CTGTGCTTCA CCAGAAGACC 
CTGCAGGCCA TGAAGAGCCA CTCAGAAAAG GCCCATGGCC ATGGAGCTTC AAGGAAAGAA 
ACCCCCCAGT TTTTTCCTTC TAGTCCTCCA CCACATTCCC CACTAAGTCA CGGGCATATC 
CCCAGCGCCA TTGTTCTTCC TAATGCTACC CATGACTACA ACACTTCTTT CTCAAACTCC 
AATGCTCACA AAGCTGAAAA GAAGCTTCAA AACATTGACC ATCCTCTTAC AAAGTCATCC 
AGTAAAAGAG ATCACCGGCG TTCTGTGGAT TCCAGAAATA CCCTCAATGA TCTCCTGAAG 
CATCTAAATG ACCCAAACAG TAACCCCAAA GCCATCATGG GAGACATCCA GATGGCCCAC 
CAGACCCTAA TGCTGGATCC CGTGGGACCT ATGTCTGAAG TCCCGCCCAA GGTCCCTAAC 
CGCGAGGCAT CTCTCTACTC TCCTCCCTCG ACTCTTCCCA GAAATAGCCC AACCAAGCGA 
GTGGATGTTC CCACCACTCC TGGAGTTCCG ATGACTTCTT TGGAAAGACA GAGGGGTTAC 
CATAAAAATT CCTCCCAGAG GCACTCTATA TCGGCTATGC CTAAAAACTT AAGTTCACCA 
AATGGTGTTT TGTTATCTAG ACAGCCTAGT ATGAACCGTG GAGGGTACGT GCCCACCCCT 
GCAGGGCCAA AGGTGGACTA TATTCAGGGA GCACCAGTGA GTGCTCACCT ACAGCCTTCC 
CTCTCCAGAC AGAGCAGCTA CACCAGTAAT GGCACCCTTC CCCGGACGGG ACTAAAGAGG 
ACACCGTCCT TAAAACCTGA CGTGCCGCCA AAGCCTTCAT TTGTTCCTCA AACCACCTCC 
GTCAGACCAC TGAACAAATA CACTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853305.1
CDS	180..3389
Translation	MRFFLLCAYMLLLLISQLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPKTEVIPNKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYKLNTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDETLSFIKSHPLMDSAVPPIADEPWFTKTRIRYRLTAIAVDHSAGPHQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNHAKCSAENEEDRKVISLQLDKDHHALYVAFSSCVIRIPLSRCERYGSCKKSCIASRDPYCGWLSQGACGRVSPAMLAGGFEQDTEYGNTAHLGDCHEILPTSTTPDYKIFGGPTSDMEVSSSSVTTMASIPEITPKVIDTWRPKLTSSRKFVVQDDPNTSDFTDPLSGVRWEVQSGEANQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNGDTKSMVMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHGHGASRKETPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNIDHPLTKSSSKRDHRRSVDSRNTLNDLLKHLNDPNSNPKAIMGDIQMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAMPKNLSSPNGVLLSRQPSMNRGGYVPTPAGPKVDYIQGAPVSAHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_024997537.1
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 6D (SEMA6D), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997537.1

ORF Insert Sequence:

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC 
AACACGGCCC ATCTAGGGGA CTGCCACGAA ATTTTGCCTA CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCTT CATCTTCTGT TACCACAATG 
GCAAGTATCC CAGAAATTAC ACCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACCAGC 
TCCCGGAAAT TTGTAGTTCA AGATGACCCA AACACTTCTG ATTTTACTGA TCCTTTATCA 
GGTGTACGGT GGGAAGTCCA GTCTGGAGAG GCCAACCAGA TGGTCCACAT GAATGTCCTC 
ATCACCTGTG TCTTTGCAGC TTTTGTCTTG GGTGCGTTCA TTGCAGGTGT GGCAGTCTAC 
TGTTACCGTG ACATGTTTGT TCGGAAAAAC AGAAAGATCC ATAAAGATGC AGAATCTGCC 
CAGTCATGCA CAGACTCCAG TGGAAGTTTT GCCAAGCTGA ATGGTCTCTT TGACAGCCCC 
GTCAAGGAAT ACCAACAGAA TATTGATTCT CCCAAATTAT ATAGTAACCT GCTGACCAGT 
CGGAAAGAGC TGCCACCCAA TGGAGATACA AAATCCATGG TCATGGACCA TCGAGGCCAA 
CCTCCTGAGT TGGCTGCTCT CCCCACTCCT GAGTCTACGC CTGTGCTTCA CCAGAAGACC 
CTGCAGGCCA TGAAGAGCCA CTCAGAAAAG GCCCATGGCC ATGGAGCTTC AAGGAAAGAA 
ACCCCCCAGT TTTTTCCTTC TAGTCCTCCA CCACATTCCC CACTAAGTCA CGGGCATATC 
CCCAGCGCCA TTGTTCTTCC TAATGCTACC CATGACTACA ACACTTCTTT CTCAAACTCC 
AATGCTCACA AAGCTGAAAA GAAGCTTCAA AACATTGACC ATCCTCTTAC AAAGTCATCC 
AGTAAAAGAG ATCACCGGCG TTCTGTGGAT TCCAGAAATA CCCTCAATGA TCTCCTGAAG 
CATCTAAATG ACCCAAACAG TAACCCCAAA GCCATCATGG GAGACATCCA GATGGCCCAC 
CAGACCCTAA TGCTGGATCC CGTGGGACCT ATGTCTGAAG TCCCGCCCAA GGTCCCTAAC 
CGCGAGGCAT CTCTCTACTC TCCTCCCTCG ACTCTTCCCA GAAATAGCCC AACCAAGCGA 
GTGGATGTTC CCACCACTCC TGGAGTTCCG ATGACTTCTT TGGAAAGACA GAGGGGTTAC 
CATAAAAATT CCTCCCAGAG GCACTCTATA TCGGCTATGC CTAAAAACTT AAGTTCACCA 
AATGGTGTTT TGTTATCTAG ACAGCCTAGT ATGAACCGTG GAGGGTACGT GCCCACCCCT 
GCAGGGCCAA AGGTGGACTA TATTCAGGGA GCACCAGTGA GTGCTCACCT ACAGCCTTCC 
CTCTCCAGAC AGAGCAGCTA CACCAGTAAT GGCACCCTTC CCCGGACGGG ACTAAAGAGG 
ACACCGTCCT TAAAACCTGA CGTGCCGCCA AAGCCTTCAT TTGTTCCTCA AACCACCTCC 
GTCAGACCAC TGAACAAATA CACTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA6D ( XM_024997538.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853306.1 Bos taurus semaphorin 6D (SEMA6D), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa219558
Clone ID Related Accession (Same CDS sequence)	XM_024997538.1
Accession Version	XM_024997538.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	semaphorin-6D isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC 
AACACGGCCC ATCTAGGGGA CTGCCACGAC ATGGAGGTAT CTTCATCTTC TGTTACCACA 
ATGGCAAGTA TCCCAGAAAT TACACCTAAA GTGATTGATA CCTGGAGACC TAAACTGACC 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAACACTT CTGATTTTAC TGATCCTTTA 
TCAGGTGTAC GGTGGGAAGT CCAGTCTGGA GAGGCCAACC AGATGGTCCA CATGAATGTC 
CTCATCACCT GTGTCTTTGC AGCTTTTGTC TTGGGTGCGT TCATTGCAGG TGTGGCAGTC 
TACTGTTACC GTGACATGTT TGTTCGGAAA AACAGAAAGA TCCATAAAGA TGCAGAATCT 
GCCCAGTCAT GCACAGACTC CAGTGGAAGT TTTGCCAAGC TGAATGGTCT CTTTGACAGC 
CCCGTCAAGG AATACCAACA GAATATTGAT TCTCCCAAAT TATATAGTAA CCTGCTGACC 
AGTCGGAAAG AGCTGCCACC CAATGGAGAT ACAAAATCCA TGGTCATGGA CCATCGAGGC 
CAACCTCCTG AGTTGGCTGC TCTCCCCACT CCTGAGTCTA CGCCTGTGCT TCACCAGAAG 
ACCCTGCAGG CCATGAAGAG CCACTCAGAA AAGGCCCATG GCCATGGAGC TTCAAGGAAA 
GAAACCCCCC AGTTTTTTCC TTCTAGTCCT CCACCACATT CCCCACTAAG TCACGGGCAT 
ATCCCCAGCG CCATTGTTCT TCCTAATGCT ACCCATGACT ACAACACTTC TTTCTCAAAC 
TCCAATGCTC ACAAAGCTGA AAAGAAGCTT CAAAACATTG ACCATCCTCT TACAAAGTCA 
TCCAGTAAAA GAGATCACCG GCGTTCTGTG GATTCCAGAA ATACCCTCAA TGATCTCCTG 
AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCA TGGGAGACAT CCAGATGGCC 
CACCAGACCC TAATGCTGGA TCCCGTGGGA CCTATGTCTG AAGTCCCGCC CAAGGTCCCT 
AACCGCGAGG CATCTCTCTA CTCTCCTCCC TCGACTCTTC CCAGAAATAG CCCAACCAAG 
CGAGTGGATG TTCCCACCAC TCCTGGAGTT CCGATGACTT CTTTGGAAAG ACAGAGGGGT 
TACCATAAAA ATTCCTCCCA GAGGCACTCT ATATCGGCTA TGCCTAAAAA CTTAAGTTCA 
CCAAATGGTG TTTTGTTATC TAGACAGCCT AGTATGAACC GTGGAGGGTA CGTGCCCACC 
CCTGCAGGGC CAAAGGTGGA CTATATTCAG GGAGCACCAG TGAGTGCTCA CCTACAGCCT 
TCCCTCTCCA GACAGAGCAG CTACACCAGT AATGGCACCC TTCCCCGGAC GGGACTAAAG 
AGGACACCGT CCTTAAAACC TGACGTGCCG CCAAAGCCTT CATTTGTTCC TCAAACCACC 
TCCGTCAGAC CACTGAACAA ATACACTTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853306.1
CDS	180..3332
Translation	MRFFLLCAYMLLLLISQLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPKTEVIPNKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYKLNTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDETLSFIKSHPLMDSAVPPIADEPWFTKTRIRYRLTAIAVDHSAGPHQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNHAKCSAENEEDRKVISLQLDKDHHALYVAFSSCVIRIPLSRCERYGSCKKSCIASRDPYCGWLSQGACGRVSPAMLAGGFEQDTEYGNTAHLGDCHDMEVSSSSVTTMASIPEITPKVIDTWRPKLTSSRKFVVQDDPNTSDFTDPLSGVRWEVQSGEANQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNGDTKSMVMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHGHGASRKETPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNIDHPLTKSSSKRDHRRSVDSRNTLNDLLKHLNDPNSNPKAIMGDIQMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAMPKNLSSPNGVLLSRQPSMNRGGYVPTPAGPKVDYIQGAPVSAHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_024997538.1
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 6D (SEMA6D), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997538.1

ORF Insert Sequence:

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC 
AACACGGCCC ATCTAGGGGA CTGCCACGAC ATGGAGGTAT CTTCATCTTC TGTTACCACA 
ATGGCAAGTA TCCCAGAAAT TACACCTAAA GTGATTGATA CCTGGAGACC TAAACTGACC 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAACACTT CTGATTTTAC TGATCCTTTA 
TCAGGTGTAC GGTGGGAAGT CCAGTCTGGA GAGGCCAACC AGATGGTCCA CATGAATGTC 
CTCATCACCT GTGTCTTTGC AGCTTTTGTC TTGGGTGCGT TCATTGCAGG TGTGGCAGTC 
TACTGTTACC GTGACATGTT TGTTCGGAAA AACAGAAAGA TCCATAAAGA TGCAGAATCT 
GCCCAGTCAT GCACAGACTC CAGTGGAAGT TTTGCCAAGC TGAATGGTCT CTTTGACAGC 
CCCGTCAAGG AATACCAACA GAATATTGAT TCTCCCAAAT TATATAGTAA CCTGCTGACC 
AGTCGGAAAG AGCTGCCACC CAATGGAGAT ACAAAATCCA TGGTCATGGA CCATCGAGGC 
CAACCTCCTG AGTTGGCTGC TCTCCCCACT CCTGAGTCTA CGCCTGTGCT TCACCAGAAG 
ACCCTGCAGG CCATGAAGAG CCACTCAGAA AAGGCCCATG GCCATGGAGC TTCAAGGAAA 
GAAACCCCCC AGTTTTTTCC TTCTAGTCCT CCACCACATT CCCCACTAAG TCACGGGCAT 
ATCCCCAGCG CCATTGTTCT TCCTAATGCT ACCCATGACT ACAACACTTC TTTCTCAAAC 
TCCAATGCTC ACAAAGCTGA AAAGAAGCTT CAAAACATTG ACCATCCTCT TACAAAGTCA 
TCCAGTAAAA GAGATCACCG GCGTTCTGTG GATTCCAGAA ATACCCTCAA TGATCTCCTG 
AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCA TGGGAGACAT CCAGATGGCC 
CACCAGACCC TAATGCTGGA TCCCGTGGGA CCTATGTCTG AAGTCCCGCC CAAGGTCCCT 
AACCGCGAGG CATCTCTCTA CTCTCCTCCC TCGACTCTTC CCAGAAATAG CCCAACCAAG 
CGAGTGGATG TTCCCACCAC TCCTGGAGTT CCGATGACTT CTTTGGAAAG ACAGAGGGGT 
TACCATAAAA ATTCCTCCCA GAGGCACTCT ATATCGGCTA TGCCTAAAAA CTTAAGTTCA 
CCAAATGGTG TTTTGTTATC TAGACAGCCT AGTATGAACC GTGGAGGGTA CGTGCCCACC 
CCTGCAGGGC CAAAGGTGGA CTATATTCAG GGAGCACCAG TGAGTGCTCA CCTACAGCCT 
TCCCTCTCCA GACAGAGCAG CTACACCAGT AATGGCACCC TTCCCCGGAC GGGACTAAAG 
AGGACACCGT CCTTAAAACC TGACGTGCCG CCAAAGCCTT CATTTGTTCC TCAAACCACC 
TCCGTCAGAC CACTGAACAA ATACACTTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA6D ( NM_001191133.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001178062.1 Bos taurus semaphorin 6D (SEMA6D), mRNA.

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CloneID	OBa40864
Clone ID Related Accession (Same CDS sequence)	NM_001191133.1
Accession Version	NM_001191133.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-29
Organism	Bos taurus(cattle)
Product	semaphorin-6D precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000010.1. On Jul 17, 2010 this sequence version replaced XM_001249780.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR5571258.3122.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC 
AACACGGCCC ATCTAGGGGA CTGCCACGAA ATTTTGCCTA CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCTT CATCTTCTGT TACCACAATG 
GCAAGTATCC CAGAAATTAC ACCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACCAGC 
TCCCGGAAAT TTGTAGTTCA AGATGACCCA AACACTTCTG ATTTTACTGA TCCTTTATCA 
GGTATCCCAA AGGGTGTACG GTGGGAAGTC CAGTCTGGAG AGGCCAACCA GATGGTCCAC 
ATGAATGTCC TCATCACCTG TGTCTTTGCA GCTTTTGTCT TGGGTGCGTT CATTGCAGGT 
GTGGCAGTCT ACTGTTACCG TGACATGTTT GTTCGGAAAA ACAGAAAGAT CCATAAAGAT 
GCAGAATCTG CCCAGTCATG CACAGACTCC AGTGGAAGTT TTGCCAAGCT GAATGGTCTC 
TTTGACAGCC CCGTCAAGGA ATACCAACAG AATATTGATT CTCCCAAATT ATATAGTAAC 
CTGCTGACCA GTCGGAAAGA GCTGCCACCC AATGGAGATA CAAAATCCAT GGTCATGGAC 
CATCGAGGCC AACCTCCTGA GTTGGCTGCT CTCCCCACTC CTGAGTCTAC GCCTGTGCTT 
CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCAGAAA AGGCCCATGG CCATGGAGCT 
TCAAGGAAAG AAACCCCCCA GTTTTTTCCT TCTAGTCCTC CACCACATTC CCCACTAAGT 
CACGGGCATA TCCCCAGCGC CATTGTTCTT CCTAATGCTA CCCATGACTA CAACACTTCT 
TTCTCAAACT CCAATGCTCA CAAAGCTGAA AAGAAGCTTC AAAACATTGA CCATCCTCTT 
ACAAAGTCAT CCAGTAAAAG AGATCACCGG CGTTCTGTGG ATTCCAGAAA TACCCTCAAT 
GATCTCCTGA AGCATCTAAA TGACCCAAAC AGTAACCCCA AAGCCATCAT GGGAGACATC 
CAGATGGCCC ACCAGACCCT AATGCTGGAT CCCGTGGGAC CTATGTCTGA AGTCCCGCCC 
AAGGTCCCTA ACCGCGAGGC ATCTCTCTAC TCTCCTCCCT CGACTCTTCC CAGAAATAGC 
CCAACCAAGC GAGTGGATGT TCCCACCACT CCTGGAGTTC CGATGACTTC TTTGGAAAGA 
CAGAGGGGTT ACCATAAAAA TTCCTCCCAG AGGCACTCTA TATCGGCTAT GCCTAAAAAC 
TTAAGTTCAC CAAATGGTGT TTTGTTATCT AGACAGCCTA GTATGAACCG TGGAGGGTAC 
GTGCCCACCC CTGCAGGGCC AAAGGTGGAC TATATTCAGG GAGCACCAGT GAGTGCTCAC 
CTACAGCCTT CCCTCTCCAG ACAGAGCAGC TACACCAGTA ATGGCACCCT TCCCCGGACG 
GGACTAAAGA GGACACCGTC CTTAAAACCT GACGTGCCGC CAAAGCCTTC ATTTGTTCCT 
CAAACCACCT CCGTCAGACC ACTGAACAAA TACACTTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178062.1
CDS	350..3571
Translation	MRFFLLCAYMLLLLISQLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPKTEVIPNKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYKLNTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDETLSFIKSHPLMDSAVPPIADEPWFTKTRIRYRLTAIAVDHSAGPHQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNHAKCSAENEEDRKVISLQLDKDHHALYVAFSSCVIRIPLSRCERYGSCKKSCIASRDPYCGWLSQGACGRVSPAMLAGGFEQDTEYGNTAHLGDCHEILPTSTTPDYKIFGGPTSDMEVSSSSVTTMASIPEITPKVIDTWRPKLTSSRKFVVQDDPNTSDFTDPLSGIPKGVRWEVQSGEANQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNGDTKSMVMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHGHGASRKETPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNIDHPLTKSSSKRDHRRSVDSRNTLNDLLKHLNDPNSNPKAIMGDIQMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAMPKNLSSPNGVLLSRQPSMNRGGYVPTPAGPKVDYIQGAPVSAHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	NM_001191133.1
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 6D (SEMA6D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191133.1

ORF Insert Sequence:

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC 
AACACGGCCC ATCTAGGGGA CTGCCACGAA ATTTTGCCTA CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCTT CATCTTCTGT TACCACAATG 
GCAAGTATCC CAGAAATTAC ACCTAAAGTG ATTGATACCT GGAGACCTAA ACTGACCAGC 
TCCCGGAAAT TTGTAGTTCA AGATGACCCA AACACTTCTG ATTTTACTGA TCCTTTATCA 
GGTATCCCAA AGGGTGTACG GTGGGAAGTC CAGTCTGGAG AGGCCAACCA GATGGTCCAC 
ATGAATGTCC TCATCACCTG TGTCTTTGCA GCTTTTGTCT TGGGTGCGTT CATTGCAGGT 
GTGGCAGTCT ACTGTTACCG TGACATGTTT GTTCGGAAAA ACAGAAAGAT CCATAAAGAT 
GCAGAATCTG CCCAGTCATG CACAGACTCC AGTGGAAGTT TTGCCAAGCT GAATGGTCTC 
TTTGACAGCC CCGTCAAGGA ATACCAACAG AATATTGATT CTCCCAAATT ATATAGTAAC 
CTGCTGACCA GTCGGAAAGA GCTGCCACCC AATGGAGATA CAAAATCCAT GGTCATGGAC 
CATCGAGGCC AACCTCCTGA GTTGGCTGCT CTCCCCACTC CTGAGTCTAC GCCTGTGCTT 
CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCAGAAA AGGCCCATGG CCATGGAGCT 
TCAAGGAAAG AAACCCCCCA GTTTTTTCCT TCTAGTCCTC CACCACATTC CCCACTAAGT 
CACGGGCATA TCCCCAGCGC CATTGTTCTT CCTAATGCTA CCCATGACTA CAACACTTCT 
TTCTCAAACT CCAATGCTCA CAAAGCTGAA AAGAAGCTTC AAAACATTGA CCATCCTCTT 
ACAAAGTCAT CCAGTAAAAG AGATCACCGG CGTTCTGTGG ATTCCAGAAA TACCCTCAAT 
GATCTCCTGA AGCATCTAAA TGACCCAAAC AGTAACCCCA AAGCCATCAT GGGAGACATC 
CAGATGGCCC ACCAGACCCT AATGCTGGAT CCCGTGGGAC CTATGTCTGA AGTCCCGCCC 
AAGGTCCCTA ACCGCGAGGC ATCTCTCTAC TCTCCTCCCT CGACTCTTCC CAGAAATAGC 
CCAACCAAGC GAGTGGATGT TCCCACCACT CCTGGAGTTC CGATGACTTC TTTGGAAAGA 
CAGAGGGGTT ACCATAAAAA TTCCTCCCAG AGGCACTCTA TATCGGCTAT GCCTAAAAAC 
TTAAGTTCAC CAAATGGTGT TTTGTTATCT AGACAGCCTA GTATGAACCG TGGAGGGTAC 
GTGCCCACCC CTGCAGGGCC AAAGGTGGAC TATATTCAGG GAGCACCAGT GAGTGCTCAC 
CTACAGCCTT CCCTCTCCAG ACAGAGCAGC TACACCAGTA ATGGCACCCT TCCCCGGACG 
GGACTAAAGA GGACACCGTC CTTAAAACCT GACGTGCCGC CAAAGCCTTC ATTTGTTCCT 
CAAACCACCT CCGTCAGACC ACTGAACAAA TACACTTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA6D ( XM_005211812.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211869.1 Bos taurus semaphorin 6D (SEMA6D), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa23344
Clone ID Related Accession (Same CDS sequence)	XM_005211812.3 , XM_005211812.4
Accession Version	XM_005211812.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	semaphorin-6D isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211812.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC 
CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC 
TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGGTGTAC GGTGGGAAGT CCAGTCTGGA GAGGCCAACC AGATGGTCCA CATGAATGTC 
CTCATCACCT GTGTCTTTGC AGCTTTTGTC TTGGGTGCGT TCATTGCAGG TGTGGCAGTC 
TACTGTTACC GTGACATGTT TGTTCGGAAA AACAGAAAGA TCCATAAAGA TGCAGAATCT 
GCCCAGTCAT GCACAGACTC CAGTGGAAGT TTTGCCAAGC TGAATGGTCT CTTTGACAGC 
CCCGTCAAGG AATACCAACA GAATATTGAT TCTCCCAAAT TATATAGTAA CCTGCTGACC 
AGTCGGAAAG AGCTGCCACC CAATGGAGAT ACAAAATCCA TGGTCATGGA CCATCGAGGC 
CAACCTCCTG AGTTGGCTGC TCTCCCCACT CCTGAGTCTA CGCCTGTGCT TCACCAGAAG 
ACCCTGCAGG CCATGAAGAG CCACTCAGAA AAGGCCCATG GCCATGGAGC TTCAAGGAAA 
GAAACCCCCC AGTTTTTTCC TTCTAGTCCT CCACCACATT CCCCACTAAG TCACGGGCAT 
ATCCCCAGCG CCATTGTTCT TCCTAATGCT ACCCATGACT ACAACACTTC TTTCTCAAAC 
TCCAATGCTC ACAAAGCTGA AAAGAAGCTT CAAAACATTG ACCATCCTCT TACAAAGTCA 
TCCAGTAAAA GAGATCACCG GCGTTCTGTG GATTCCAGAA ATACCCTCAA TGATCTCCTG 
AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCA TGGGAGACAT CCAGATGGCC 
CACCAGACCC TAATGCTGGA TCCCGTGGGA CCTATGTCTG AAGTCCCGCC CAAGGTCCCT 
AACCGCGAGG CATCTCTCTA CTCTCCTCCC TCGACTCTTC CCAGAAATAG CCCAACCAAG 
CGAGTGGATG TTCCCACCAC TCCTGGAGTT CCGATGACTT CTTTGGAAAG ACAGAGGGGT 
TACCATAAAA ATTCCTCCCA GAGGCACTCT ATATCGGCTA TGCCTAAAAA CTTAAGTTCA 
CCAAATGGTG TTTTGTTATC TAGACAGCCT AGTATGAACC GTGGAGGGTA CGTGCCCACC 
CCTGCAGGGC CAAAGGTGGA CTATATTCAG GGAGCACCAG TGAGTGCTCA CCTACAGCCT 
TCCCTCTCCA GACAGAGCAG CTACACCAGT AATGGCACCC TTCCCCGGAC GGGACTAAAG 
AGGACACCGT CCTTAAAACC TGACGTGCCG CCAAAGCCTT CATTTGTTCC TCAAACCACC 
TCCGTCAGAC CACTGAACAA ATACACTTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211869.1
CDS	345..3437
Translation	MRFFLLCAYMLLLLISQLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPKTEVIPNKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYKLNTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDETLSFIKSHPLMDSAVPPIADEPWFTKTRIRYRLTAIAVDHSAGPHQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNHAKCSAENEEDRKVISLQLDKDHHALYVAFSSCVIRIPLSRCERYGSCKKSCIASRDPYCGWLSQGACGRVSPAMLLLTEDLFAFHNHSAGGFEQDTEYGNTAHLGDCHEILPTSTTPDYKIFGGPTSGVRWEVQSGEANQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNGDTKSMVMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHGHGASRKETPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNIDHPLTKSSSKRDHRRSVDSRNTLNDLLKHLNDPNSNPKAIMGDIQMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAMPKNLSSPNGVLLSRQPSMNRGGYVPTPAGPKVDYIQGAPVSAHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_005211812.3
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 6D (SEMA6D), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211812.3

ORF Insert Sequence:

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC 
CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC 
TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGGTGTAC GGTGGGAAGT CCAGTCTGGA GAGGCCAACC AGATGGTCCA CATGAATGTC 
CTCATCACCT GTGTCTTTGC AGCTTTTGTC TTGGGTGCGT TCATTGCAGG TGTGGCAGTC 
TACTGTTACC GTGACATGTT TGTTCGGAAA AACAGAAAGA TCCATAAAGA TGCAGAATCT 
GCCCAGTCAT GCACAGACTC CAGTGGAAGT TTTGCCAAGC TGAATGGTCT CTTTGACAGC 
CCCGTCAAGG AATACCAACA GAATATTGAT TCTCCCAAAT TATATAGTAA CCTGCTGACC 
AGTCGGAAAG AGCTGCCACC CAATGGAGAT ACAAAATCCA TGGTCATGGA CCATCGAGGC 
CAACCTCCTG AGTTGGCTGC TCTCCCCACT CCTGAGTCTA CGCCTGTGCT TCACCAGAAG 
ACCCTGCAGG CCATGAAGAG CCACTCAGAA AAGGCCCATG GCCATGGAGC TTCAAGGAAA 
GAAACCCCCC AGTTTTTTCC TTCTAGTCCT CCACCACATT CCCCACTAAG TCACGGGCAT 
ATCCCCAGCG CCATTGTTCT TCCTAATGCT ACCCATGACT ACAACACTTC TTTCTCAAAC 
TCCAATGCTC ACAAAGCTGA AAAGAAGCTT CAAAACATTG ACCATCCTCT TACAAAGTCA 
TCCAGTAAAA GAGATCACCG GCGTTCTGTG GATTCCAGAA ATACCCTCAA TGATCTCCTG 
AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCA TGGGAGACAT CCAGATGGCC 
CACCAGACCC TAATGCTGGA TCCCGTGGGA CCTATGTCTG AAGTCCCGCC CAAGGTCCCT 
AACCGCGAGG CATCTCTCTA CTCTCCTCCC TCGACTCTTC CCAGAAATAG CCCAACCAAG 
CGAGTGGATG TTCCCACCAC TCCTGGAGTT CCGATGACTT CTTTGGAAAG ACAGAGGGGT 
TACCATAAAA ATTCCTCCCA GAGGCACTCT ATATCGGCTA TGCCTAAAAA CTTAAGTTCA 
CCAAATGGTG TTTTGTTATC TAGACAGCCT AGTATGAACC GTGGAGGGTA CGTGCCCACC 
CCTGCAGGGC CAAAGGTGGA CTATATTCAG GGAGCACCAG TGAGTGCTCA CCTACAGCCT 
TCCCTCTCCA GACAGAGCAG CTACACCAGT AATGGCACCC TTCCCCGGAC GGGACTAAAG 
AGGACACCGT CCTTAAAACC TGACGTGCCG CCAAAGCCTT CATTTGTTCC TCAAACCACC 
TCCGTCAGAC CACTGAACAA ATACACTTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA6D ( XM_005211812.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211869.1 Bos taurus semaphorin 6D (SEMA6D), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa23344
Clone ID Related Accession (Same CDS sequence)	XM_005211812.3 , XM_005211812.4
Accession Version	XM_005211812.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	semaphorin-6D isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211812.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC 
CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC 
TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGGTGTAC GGTGGGAAGT CCAGTCTGGA GAGGCCAACC AGATGGTCCA CATGAATGTC 
CTCATCACCT GTGTCTTTGC AGCTTTTGTC TTGGGTGCGT TCATTGCAGG TGTGGCAGTC 
TACTGTTACC GTGACATGTT TGTTCGGAAA AACAGAAAGA TCCATAAAGA TGCAGAATCT 
GCCCAGTCAT GCACAGACTC CAGTGGAAGT TTTGCCAAGC TGAATGGTCT CTTTGACAGC 
CCCGTCAAGG AATACCAACA GAATATTGAT TCTCCCAAAT TATATAGTAA CCTGCTGACC 
AGTCGGAAAG AGCTGCCACC CAATGGAGAT ACAAAATCCA TGGTCATGGA CCATCGAGGC 
CAACCTCCTG AGTTGGCTGC TCTCCCCACT CCTGAGTCTA CGCCTGTGCT TCACCAGAAG 
ACCCTGCAGG CCATGAAGAG CCACTCAGAA AAGGCCCATG GCCATGGAGC TTCAAGGAAA 
GAAACCCCCC AGTTTTTTCC TTCTAGTCCT CCACCACATT CCCCACTAAG TCACGGGCAT 
ATCCCCAGCG CCATTGTTCT TCCTAATGCT ACCCATGACT ACAACACTTC TTTCTCAAAC 
TCCAATGCTC ACAAAGCTGA AAAGAAGCTT CAAAACATTG ACCATCCTCT TACAAAGTCA 
TCCAGTAAAA GAGATCACCG GCGTTCTGTG GATTCCAGAA ATACCCTCAA TGATCTCCTG 
AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCA TGGGAGACAT CCAGATGGCC 
CACCAGACCC TAATGCTGGA TCCCGTGGGA CCTATGTCTG AAGTCCCGCC CAAGGTCCCT 
AACCGCGAGG CATCTCTCTA CTCTCCTCCC TCGACTCTTC CCAGAAATAG CCCAACCAAG 
CGAGTGGATG TTCCCACCAC TCCTGGAGTT CCGATGACTT CTTTGGAAAG ACAGAGGGGT 
TACCATAAAA ATTCCTCCCA GAGGCACTCT ATATCGGCTA TGCCTAAAAA CTTAAGTTCA 
CCAAATGGTG TTTTGTTATC TAGACAGCCT AGTATGAACC GTGGAGGGTA CGTGCCCACC 
CCTGCAGGGC CAAAGGTGGA CTATATTCAG GGAGCACCAG TGAGTGCTCA CCTACAGCCT 
TCCCTCTCCA GACAGAGCAG CTACACCAGT AATGGCACCC TTCCCCGGAC GGGACTAAAG 
AGGACACCGT CCTTAAAACC TGACGTGCCG CCAAAGCCTT CATTTGTTCC TCAAACCACC 
TCCGTCAGAC CACTGAACAA ATACACTTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211869.1
CDS	180..3272
Translation	MRFFLLCAYMLLLLISQLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPKTEVIPNKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYKLNTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDETLSFIKSHPLMDSAVPPIADEPWFTKTRIRYRLTAIAVDHSAGPHQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNHAKCSAENEEDRKVISLQLDKDHHALYVAFSSCVIRIPLSRCERYGSCKKSCIASRDPYCGWLSQGACGRVSPAMLLLTEDLFAFHNHSAGGFEQDTEYGNTAHLGDCHEILPTSTTPDYKIFGGPTSGVRWEVQSGEANQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNGDTKSMVMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHGHGASRKETPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNIDHPLTKSSSKRDHRRSVDSRNTLNDLLKHLNDPNSNPKAIMGDIQMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAMPKNLSSPNGVLLSRQPSMNRGGYVPTPAGPKVDYIQGAPVSAHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_005211812.4
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 6D (SEMA6D), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211812.4

ORF Insert Sequence:

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC 
CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC 
TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGGTGTAC GGTGGGAAGT CCAGTCTGGA GAGGCCAACC AGATGGTCCA CATGAATGTC 
CTCATCACCT GTGTCTTTGC AGCTTTTGTC TTGGGTGCGT TCATTGCAGG TGTGGCAGTC 
TACTGTTACC GTGACATGTT TGTTCGGAAA AACAGAAAGA TCCATAAAGA TGCAGAATCT 
GCCCAGTCAT GCACAGACTC CAGTGGAAGT TTTGCCAAGC TGAATGGTCT CTTTGACAGC 
CCCGTCAAGG AATACCAACA GAATATTGAT TCTCCCAAAT TATATAGTAA CCTGCTGACC 
AGTCGGAAAG AGCTGCCACC CAATGGAGAT ACAAAATCCA TGGTCATGGA CCATCGAGGC 
CAACCTCCTG AGTTGGCTGC TCTCCCCACT CCTGAGTCTA CGCCTGTGCT TCACCAGAAG 
ACCCTGCAGG CCATGAAGAG CCACTCAGAA AAGGCCCATG GCCATGGAGC TTCAAGGAAA 
GAAACCCCCC AGTTTTTTCC TTCTAGTCCT CCACCACATT CCCCACTAAG TCACGGGCAT 
ATCCCCAGCG CCATTGTTCT TCCTAATGCT ACCCATGACT ACAACACTTC TTTCTCAAAC 
TCCAATGCTC ACAAAGCTGA AAAGAAGCTT CAAAACATTG ACCATCCTCT TACAAAGTCA 
TCCAGTAAAA GAGATCACCG GCGTTCTGTG GATTCCAGAA ATACCCTCAA TGATCTCCTG 
AAGCATCTAA ATGACCCAAA CAGTAACCCC AAAGCCATCA TGGGAGACAT CCAGATGGCC 
CACCAGACCC TAATGCTGGA TCCCGTGGGA CCTATGTCTG AAGTCCCGCC CAAGGTCCCT 
AACCGCGAGG CATCTCTCTA CTCTCCTCCC TCGACTCTTC CCAGAAATAG CCCAACCAAG 
CGAGTGGATG TTCCCACCAC TCCTGGAGTT CCGATGACTT CTTTGGAAAG ACAGAGGGGT 
TACCATAAAA ATTCCTCCCA GAGGCACTCT ATATCGGCTA TGCCTAAAAA CTTAAGTTCA 
CCAAATGGTG TTTTGTTATC TAGACAGCCT AGTATGAACC GTGGAGGGTA CGTGCCCACC 
CCTGCAGGGC CAAAGGTGGA CTATATTCAG GGAGCACCAG TGAGTGCTCA CCTACAGCCT 
TCCCTCTCCA GACAGAGCAG CTACACCAGT AATGGCACCC TTCCCCGGAC GGGACTAAAG 
AGGACACCGT CCTTAAAACC TGACGTGCCG CCAAAGCCTT CATTTGTTCC TCAAACCACC 
TCCGTCAGAC CACTGAACAA ATACACTTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA6D ( XM_005211813.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211870.1 Bos taurus semaphorin 6D (SEMA6D), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa23345
Clone ID Related Accession (Same CDS sequence)	XM_005211813.3 , XM_005211813.4
Accession Version	XM_005211813.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	semaphorin-6D isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211813.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC 
AACACGGCCC ATCTAGGGGA CTGCCACGAA ATTTTGCCTA CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGGTGTA CGGTGGGAAG TCCAGTCTGG AGAGGCCAAC 
CAGATGGTCC ACATGAATGT CCTCATCACC TGTGTCTTTG CAGCTTTTGT CTTGGGTGCG 
TTCATTGCAG GTGTGGCAGT CTACTGTTAC CGTGACATGT TTGTTCGGAA AAACAGAAAG 
ATCCATAAAG ATGCAGAATC TGCCCAGTCA TGCACAGACT CCAGTGGAAG TTTTGCCAAG 
CTGAATGGTC TCTTTGACAG CCCCGTCAAG GAATACCAAC AGAATATTGA TTCTCCCAAA 
TTATATAGTA ACCTGCTGAC CAGTCGGAAA GAGCTGCCAC CCAATGGAGA TACAAAATCC 
ATGGTCATGG ACCATCGAGG CCAACCTCCT GAGTTGGCTG CTCTCCCCAC TCCTGAGTCT 
ACGCCTGTGC TTCACCAGAA GACCCTGCAG GCCATGAAGA GCCACTCAGA AAAGGCCCAT 
GGCCATGGAG CTTCAAGGAA AGAAACCCCC CAGTTTTTTC CTTCTAGTCC TCCACCACAT 
TCCCCACTAA GTCACGGGCA TATCCCCAGC GCCATTGTTC TTCCTAATGC TACCCATGAC 
TACAACACTT CTTTCTCAAA CTCCAATGCT CACAAAGCTG AAAAGAAGCT TCAAAACATT 
GACCATCCTC TTACAAAGTC ATCCAGTAAA AGAGATCACC GGCGTTCTGT GGATTCCAGA 
AATACCCTCA ATGATCTCCT GAAGCATCTA AATGACCCAA ACAGTAACCC CAAAGCCATC 
ATGGGAGACA TCCAGATGGC CCACCAGACC CTAATGCTGG ATCCCGTGGG ACCTATGTCT 
GAAGTCCCGC CCAAGGTCCC TAACCGCGAG GCATCTCTCT ACTCTCCTCC CTCGACTCTT 
CCCAGAAATA GCCCAACCAA GCGAGTGGAT GTTCCCACCA CTCCTGGAGT TCCGATGACT 
TCTTTGGAAA GACAGAGGGG TTACCATAAA AATTCCTCCC AGAGGCACTC TATATCGGCT 
ATGCCTAAAA ACTTAAGTTC ACCAAATGGT GTTTTGTTAT CTAGACAGCC TAGTATGAAC 
CGTGGAGGGT ACGTGCCCAC CCCTGCAGGG CCAAAGGTGG ACTATATTCA GGGAGCACCA 
GTGAGTGCTC ACCTACAGCC TTCCCTCTCC AGACAGAGCA GCTACACCAG TAATGGCACC 
CTTCCCCGGA CGGGACTAAA GAGGACACCG TCCTTAAAAC CTGACGTGCC GCCAAAGCCT 
TCATTTGTTC CTCAAACCAC CTCCGTCAGA CCACTGAACA AATACACTTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211870.1
CDS	345..3398
Translation	MRFFLLCAYMLLLLISQLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPKTEVIPNKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYKLNTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDETLSFIKSHPLMDSAVPPIADEPWFTKTRIRYRLTAIAVDHSAGPHQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNHAKCSAENEEDRKVISLQLDKDHHALYVAFSSCVIRIPLSRCERYGSCKKSCIASRDPYCGWLSQGACGRVSPAMLAGGFEQDTEYGNTAHLGDCHEILPTSTTPDYKIFGGPTSGVRWEVQSGEANQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNGDTKSMVMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHGHGASRKETPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNIDHPLTKSSSKRDHRRSVDSRNTLNDLLKHLNDPNSNPKAIMGDIQMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAMPKNLSSPNGVLLSRQPSMNRGGYVPTPAGPKVDYIQGAPVSAHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_005211813.3
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 6D (SEMA6D), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211813.3

ORF Insert Sequence:

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC 
AACACGGCCC ATCTAGGGGA CTGCCACGAA ATTTTGCCTA CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGGTGTA CGGTGGGAAG TCCAGTCTGG AGAGGCCAAC 
CAGATGGTCC ACATGAATGT CCTCATCACC TGTGTCTTTG CAGCTTTTGT CTTGGGTGCG 
TTCATTGCAG GTGTGGCAGT CTACTGTTAC CGTGACATGT TTGTTCGGAA AAACAGAAAG 
ATCCATAAAG ATGCAGAATC TGCCCAGTCA TGCACAGACT CCAGTGGAAG TTTTGCCAAG 
CTGAATGGTC TCTTTGACAG CCCCGTCAAG GAATACCAAC AGAATATTGA TTCTCCCAAA 
TTATATAGTA ACCTGCTGAC CAGTCGGAAA GAGCTGCCAC CCAATGGAGA TACAAAATCC 
ATGGTCATGG ACCATCGAGG CCAACCTCCT GAGTTGGCTG CTCTCCCCAC TCCTGAGTCT 
ACGCCTGTGC TTCACCAGAA GACCCTGCAG GCCATGAAGA GCCACTCAGA AAAGGCCCAT 
GGCCATGGAG CTTCAAGGAA AGAAACCCCC CAGTTTTTTC CTTCTAGTCC TCCACCACAT 
TCCCCACTAA GTCACGGGCA TATCCCCAGC GCCATTGTTC TTCCTAATGC TACCCATGAC 
TACAACACTT CTTTCTCAAA CTCCAATGCT CACAAAGCTG AAAAGAAGCT TCAAAACATT 
GACCATCCTC TTACAAAGTC ATCCAGTAAA AGAGATCACC GGCGTTCTGT GGATTCCAGA 
AATACCCTCA ATGATCTCCT GAAGCATCTA AATGACCCAA ACAGTAACCC CAAAGCCATC 
ATGGGAGACA TCCAGATGGC CCACCAGACC CTAATGCTGG ATCCCGTGGG ACCTATGTCT 
GAAGTCCCGC CCAAGGTCCC TAACCGCGAG GCATCTCTCT ACTCTCCTCC CTCGACTCTT 
CCCAGAAATA GCCCAACCAA GCGAGTGGAT GTTCCCACCA CTCCTGGAGT TCCGATGACT 
TCTTTGGAAA GACAGAGGGG TTACCATAAA AATTCCTCCC AGAGGCACTC TATATCGGCT 
ATGCCTAAAA ACTTAAGTTC ACCAAATGGT GTTTTGTTAT CTAGACAGCC TAGTATGAAC 
CGTGGAGGGT ACGTGCCCAC CCCTGCAGGG CCAAAGGTGG ACTATATTCA GGGAGCACCA 
GTGAGTGCTC ACCTACAGCC TTCCCTCTCC AGACAGAGCA GCTACACCAG TAATGGCACC 
CTTCCCCGGA CGGGACTAAA GAGGACACCG TCCTTAAAAC CTGACGTGCC GCCAAAGCCT 
TCATTTGTTC CTCAAACCAC CTCCGTCAGA CCACTGAACA AATACACTTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA6D ( XM_005211813.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211870.1 Bos taurus semaphorin 6D (SEMA6D), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBa23345
Clone ID Related Accession (Same CDS sequence)	XM_005211813.3 , XM_005211813.4
Accession Version	XM_005211813.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	semaphorin-6D isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211813.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC 
AACACGGCCC ATCTAGGGGA CTGCCACGAA ATTTTGCCTA CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGGTGTA CGGTGGGAAG TCCAGTCTGG AGAGGCCAAC 
CAGATGGTCC ACATGAATGT CCTCATCACC TGTGTCTTTG CAGCTTTTGT CTTGGGTGCG 
TTCATTGCAG GTGTGGCAGT CTACTGTTAC CGTGACATGT TTGTTCGGAA AAACAGAAAG 
ATCCATAAAG ATGCAGAATC TGCCCAGTCA TGCACAGACT CCAGTGGAAG TTTTGCCAAG 
CTGAATGGTC TCTTTGACAG CCCCGTCAAG GAATACCAAC AGAATATTGA TTCTCCCAAA 
TTATATAGTA ACCTGCTGAC CAGTCGGAAA GAGCTGCCAC CCAATGGAGA TACAAAATCC 
ATGGTCATGG ACCATCGAGG CCAACCTCCT GAGTTGGCTG CTCTCCCCAC TCCTGAGTCT 
ACGCCTGTGC TTCACCAGAA GACCCTGCAG GCCATGAAGA GCCACTCAGA AAAGGCCCAT 
GGCCATGGAG CTTCAAGGAA AGAAACCCCC CAGTTTTTTC CTTCTAGTCC TCCACCACAT 
TCCCCACTAA GTCACGGGCA TATCCCCAGC GCCATTGTTC TTCCTAATGC TACCCATGAC 
TACAACACTT CTTTCTCAAA CTCCAATGCT CACAAAGCTG AAAAGAAGCT TCAAAACATT 
GACCATCCTC TTACAAAGTC ATCCAGTAAA AGAGATCACC GGCGTTCTGT GGATTCCAGA 
AATACCCTCA ATGATCTCCT GAAGCATCTA AATGACCCAA ACAGTAACCC CAAAGCCATC 
ATGGGAGACA TCCAGATGGC CCACCAGACC CTAATGCTGG ATCCCGTGGG ACCTATGTCT 
GAAGTCCCGC CCAAGGTCCC TAACCGCGAG GCATCTCTCT ACTCTCCTCC CTCGACTCTT 
CCCAGAAATA GCCCAACCAA GCGAGTGGAT GTTCCCACCA CTCCTGGAGT TCCGATGACT 
TCTTTGGAAA GACAGAGGGG TTACCATAAA AATTCCTCCC AGAGGCACTC TATATCGGCT 
ATGCCTAAAA ACTTAAGTTC ACCAAATGGT GTTTTGTTAT CTAGACAGCC TAGTATGAAC 
CGTGGAGGGT ACGTGCCCAC CCCTGCAGGG CCAAAGGTGG ACTATATTCA GGGAGCACCA 
GTGAGTGCTC ACCTACAGCC TTCCCTCTCC AGACAGAGCA GCTACACCAG TAATGGCACC 
CTTCCCCGGA CGGGACTAAA GAGGACACCG TCCTTAAAAC CTGACGTGCC GCCAAAGCCT 
TCATTTGTTC CTCAAACCAC CTCCGTCAGA CCACTGAACA AATACACTTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211870.1
CDS	180..3233
Translation	MRFFLLCAYMLLLLISQLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPKTEVIPNKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYKLNTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDETLSFIKSHPLMDSAVPPIADEPWFTKTRIRYRLTAIAVDHSAGPHQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNHAKCSAENEEDRKVISLQLDKDHHALYVAFSSCVIRIPLSRCERYGSCKKSCIASRDPYCGWLSQGACGRVSPAMLAGGFEQDTEYGNTAHLGDCHEILPTSTTPDYKIFGGPTSGVRWEVQSGEANQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNGDTKSMVMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHGHGASRKETPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNIDHPLTKSSSKRDHRRSVDSRNTLNDLLKHLNDPNSNPKAIMGDIQMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAMPKNLSSPNGVLLSRQPSMNRGGYVPTPAGPKVDYIQGAPVSAHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_005211813.4
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 6D (SEMA6D), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211813.4

ORF Insert Sequence:

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC 
AACACGGCCC ATCTAGGGGA CTGCCACGAA ATTTTGCCTA CTTCAACTAC ACCAGATTAC 
AAAATATTTG GCGGTCCAAC ATCTGGTGTA CGGTGGGAAG TCCAGTCTGG AGAGGCCAAC 
CAGATGGTCC ACATGAATGT CCTCATCACC TGTGTCTTTG CAGCTTTTGT CTTGGGTGCG 
TTCATTGCAG GTGTGGCAGT CTACTGTTAC CGTGACATGT TTGTTCGGAA AAACAGAAAG 
ATCCATAAAG ATGCAGAATC TGCCCAGTCA TGCACAGACT CCAGTGGAAG TTTTGCCAAG 
CTGAATGGTC TCTTTGACAG CCCCGTCAAG GAATACCAAC AGAATATTGA TTCTCCCAAA 
TTATATAGTA ACCTGCTGAC CAGTCGGAAA GAGCTGCCAC CCAATGGAGA TACAAAATCC 
ATGGTCATGG ACCATCGAGG CCAACCTCCT GAGTTGGCTG CTCTCCCCAC TCCTGAGTCT 
ACGCCTGTGC TTCACCAGAA GACCCTGCAG GCCATGAAGA GCCACTCAGA AAAGGCCCAT 
GGCCATGGAG CTTCAAGGAA AGAAACCCCC CAGTTTTTTC CTTCTAGTCC TCCACCACAT 
TCCCCACTAA GTCACGGGCA TATCCCCAGC GCCATTGTTC TTCCTAATGC TACCCATGAC 
TACAACACTT CTTTCTCAAA CTCCAATGCT CACAAAGCTG AAAAGAAGCT TCAAAACATT 
GACCATCCTC TTACAAAGTC ATCCAGTAAA AGAGATCACC GGCGTTCTGT GGATTCCAGA 
AATACCCTCA ATGATCTCCT GAAGCATCTA AATGACCCAA ACAGTAACCC CAAAGCCATC 
ATGGGAGACA TCCAGATGGC CCACCAGACC CTAATGCTGG ATCCCGTGGG ACCTATGTCT 
GAAGTCCCGC CCAAGGTCCC TAACCGCGAG GCATCTCTCT ACTCTCCTCC CTCGACTCTT 
CCCAGAAATA GCCCAACCAA GCGAGTGGAT GTTCCCACCA CTCCTGGAGT TCCGATGACT 
TCTTTGGAAA GACAGAGGGG TTACCATAAA AATTCCTCCC AGAGGCACTC TATATCGGCT 
ATGCCTAAAA ACTTAAGTTC ACCAAATGGT GTTTTGTTAT CTAGACAGCC TAGTATGAAC 
CGTGGAGGGT ACGTGCCCAC CCCTGCAGGG CCAAAGGTGG ACTATATTCA GGGAGCACCA 
GTGAGTGCTC ACCTACAGCC TTCCCTCTCC AGACAGAGCA GCTACACCAG TAATGGCACC 
CTTCCCCGGA CGGGACTAAA GAGGACACCG TCCTTAAAAC CTGACGTGCC GCCAAAGCCT 
TCATTTGTTC CTCAAACCAC CTCCGTCAGA CCACTGAACA AATACACTTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA6D ( XM_005211814.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211871.1 Bos taurus semaphorin 6D (SEMA6D), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa23346
Clone ID Related Accession (Same CDS sequence)	XM_005211814.4 , XM_005211814.3
Accession Version	XM_005211814.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	semaphorin-6D isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211814.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC 
CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC 
TGCCACGGTG TACGGTGGGA AGTCCAGTCT GGAGAGGCCA ACCAGATGGT CCACATGAAT 
GTCCTCATCA CCTGTGTCTT TGCAGCTTTT GTCTTGGGTG CGTTCATTGC AGGTGTGGCA 
GTCTACTGTT ACCGTGACAT GTTTGTTCGG AAAAACAGAA AGATCCATAA AGATGCAGAA 
TCTGCCCAGT CATGCACAGA CTCCAGTGGA AGTTTTGCCA AGCTGAATGG TCTCTTTGAC 
AGCCCCGTCA AGGAATACCA ACAGAATATT GATTCTCCCA AATTATATAG TAACCTGCTG 
ACCAGTCGGA AAGAGCTGCC ACCCAATGGA GATACAAAAT CCATGGTCAT GGACCATCGA 
GGCCAACCTC CTGAGTTGGC TGCTCTCCCC ACTCCTGAGT CTACGCCTGT GCTTCACCAG 
AAGACCCTGC AGGCCATGAA GAGCCACTCA GAAAAGGCCC ATGGCCATGG AGCTTCAAGG 
AAAGAAACCC CCCAGTTTTT TCCTTCTAGT CCTCCACCAC ATTCCCCACT AAGTCACGGG 
CATATCCCCA GCGCCATTGT TCTTCCTAAT GCTACCCATG ACTACAACAC TTCTTTCTCA 
AACTCCAATG CTCACAAAGC TGAAAAGAAG CTTCAAAACA TTGACCATCC TCTTACAAAG 
TCATCCAGTA AAAGAGATCA CCGGCGTTCT GTGGATTCCA GAAATACCCT CAATGATCTC 
CTGAAGCATC TAAATGACCC AAACAGTAAC CCCAAAGCCA TCATGGGAGA CATCCAGATG 
GCCCACCAGA CCCTAATGCT GGATCCCGTG GGACCTATGT CTGAAGTCCC GCCCAAGGTC 
CCTAACCGCG AGGCATCTCT CTACTCTCCT CCCTCGACTC TTCCCAGAAA TAGCCCAACC 
AAGCGAGTGG ATGTTCCCAC CACTCCTGGA GTTCCGATGA CTTCTTTGGA AAGACAGAGG 
GGTTACCATA AAAATTCCTC CCAGAGGCAC TCTATATCGG CTATGCCTAA AAACTTAAGT 
TCACCAAATG GTGTTTTGTT ATCTAGACAG CCTAGTATGA ACCGTGGAGG GTACGTGCCC 
ACCCCTGCAG GGCCAAAGGT GGACTATATT CAGGGAGCAC CAGTGAGTGC TCACCTACAG 
CCTTCCCTCT CCAGACAGAG CAGCTACACC AGTAATGGCA CCCTTCCCCG GACGGGACTA 
AAGAGGACAC CGTCCTTAAA ACCTGACGTG CCGCCAAAGC CTTCATTTGT TCCTCAAACC 
ACCTCCGTCA GACCACTGAA CAAATACACT TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211871.1
CDS	180..3215
Translation	MRFFLLCAYMLLLLISQLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPKTEVIPNKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYKLNTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDETLSFIKSHPLMDSAVPPIADEPWFTKTRIRYRLTAIAVDHSAGPHQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNHAKCSAENEEDRKVISLQLDKDHHALYVAFSSCVIRIPLSRCERYGSCKKSCIASRDPYCGWLSQGACGRVSPAMLLLTEDLFAFHNHSAGGFEQDTEYGNTAHLGDCHGVRWEVQSGEANQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNGDTKSMVMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHGHGASRKETPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNIDHPLTKSSSKRDHRRSVDSRNTLNDLLKHLNDPNSNPKAIMGDIQMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAMPKNLSSPNGVLLSRQPSMNRGGYVPTPAGPKVDYIQGAPVSAHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_005211814.4
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 6D (SEMA6D), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211814.4

ORF Insert Sequence:

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC 
CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC 
TGCCACGGTG TACGGTGGGA AGTCCAGTCT GGAGAGGCCA ACCAGATGGT CCACATGAAT 
GTCCTCATCA CCTGTGTCTT TGCAGCTTTT GTCTTGGGTG CGTTCATTGC AGGTGTGGCA 
GTCTACTGTT ACCGTGACAT GTTTGTTCGG AAAAACAGAA AGATCCATAA AGATGCAGAA 
TCTGCCCAGT CATGCACAGA CTCCAGTGGA AGTTTTGCCA AGCTGAATGG TCTCTTTGAC 
AGCCCCGTCA AGGAATACCA ACAGAATATT GATTCTCCCA AATTATATAG TAACCTGCTG 
ACCAGTCGGA AAGAGCTGCC ACCCAATGGA GATACAAAAT CCATGGTCAT GGACCATCGA 
GGCCAACCTC CTGAGTTGGC TGCTCTCCCC ACTCCTGAGT CTACGCCTGT GCTTCACCAG 
AAGACCCTGC AGGCCATGAA GAGCCACTCA GAAAAGGCCC ATGGCCATGG AGCTTCAAGG 
AAAGAAACCC CCCAGTTTTT TCCTTCTAGT CCTCCACCAC ATTCCCCACT AAGTCACGGG 
CATATCCCCA GCGCCATTGT TCTTCCTAAT GCTACCCATG ACTACAACAC TTCTTTCTCA 
AACTCCAATG CTCACAAAGC TGAAAAGAAG CTTCAAAACA TTGACCATCC TCTTACAAAG 
TCATCCAGTA AAAGAGATCA CCGGCGTTCT GTGGATTCCA GAAATACCCT CAATGATCTC 
CTGAAGCATC TAAATGACCC AAACAGTAAC CCCAAAGCCA TCATGGGAGA CATCCAGATG 
GCCCACCAGA CCCTAATGCT GGATCCCGTG GGACCTATGT CTGAAGTCCC GCCCAAGGTC 
CCTAACCGCG AGGCATCTCT CTACTCTCCT CCCTCGACTC TTCCCAGAAA TAGCCCAACC 
AAGCGAGTGG ATGTTCCCAC CACTCCTGGA GTTCCGATGA CTTCTTTGGA AAGACAGAGG 
GGTTACCATA AAAATTCCTC CCAGAGGCAC TCTATATCGG CTATGCCTAA AAACTTAAGT 
TCACCAAATG GTGTTTTGTT ATCTAGACAG CCTAGTATGA ACCGTGGAGG GTACGTGCCC 
ACCCCTGCAG GGCCAAAGGT GGACTATATT CAGGGAGCAC CAGTGAGTGC TCACCTACAG 
CCTTCCCTCT CCAGACAGAG CAGCTACACC AGTAATGGCA CCCTTCCCCG GACGGGACTA 
AAGAGGACAC CGTCCTTAAA ACCTGACGTG CCGCCAAAGC CTTCATTTGT TCCTCAAACC 
ACCTCCGTCA GACCACTGAA CAAATACACT TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA6D ( XM_005211814.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211871.1 Bos taurus semaphorin 6D (SEMA6D), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa23346
Clone ID Related Accession (Same CDS sequence)	XM_005211814.4 , XM_005211814.3
Accession Version	XM_005211814.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	semaphorin-6D isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211814.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC 
CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC 
TGCCACGGTG TACGGTGGGA AGTCCAGTCT GGAGAGGCCA ACCAGATGGT CCACATGAAT 
GTCCTCATCA CCTGTGTCTT TGCAGCTTTT GTCTTGGGTG CGTTCATTGC AGGTGTGGCA 
GTCTACTGTT ACCGTGACAT GTTTGTTCGG AAAAACAGAA AGATCCATAA AGATGCAGAA 
TCTGCCCAGT CATGCACAGA CTCCAGTGGA AGTTTTGCCA AGCTGAATGG TCTCTTTGAC 
AGCCCCGTCA AGGAATACCA ACAGAATATT GATTCTCCCA AATTATATAG TAACCTGCTG 
ACCAGTCGGA AAGAGCTGCC ACCCAATGGA GATACAAAAT CCATGGTCAT GGACCATCGA 
GGCCAACCTC CTGAGTTGGC TGCTCTCCCC ACTCCTGAGT CTACGCCTGT GCTTCACCAG 
AAGACCCTGC AGGCCATGAA GAGCCACTCA GAAAAGGCCC ATGGCCATGG AGCTTCAAGG 
AAAGAAACCC CCCAGTTTTT TCCTTCTAGT CCTCCACCAC ATTCCCCACT AAGTCACGGG 
CATATCCCCA GCGCCATTGT TCTTCCTAAT GCTACCCATG ACTACAACAC TTCTTTCTCA 
AACTCCAATG CTCACAAAGC TGAAAAGAAG CTTCAAAACA TTGACCATCC TCTTACAAAG 
TCATCCAGTA AAAGAGATCA CCGGCGTTCT GTGGATTCCA GAAATACCCT CAATGATCTC 
CTGAAGCATC TAAATGACCC AAACAGTAAC CCCAAAGCCA TCATGGGAGA CATCCAGATG 
GCCCACCAGA CCCTAATGCT GGATCCCGTG GGACCTATGT CTGAAGTCCC GCCCAAGGTC 
CCTAACCGCG AGGCATCTCT CTACTCTCCT CCCTCGACTC TTCCCAGAAA TAGCCCAACC 
AAGCGAGTGG ATGTTCCCAC CACTCCTGGA GTTCCGATGA CTTCTTTGGA AAGACAGAGG 
GGTTACCATA AAAATTCCTC CCAGAGGCAC TCTATATCGG CTATGCCTAA AAACTTAAGT 
TCACCAAATG GTGTTTTGTT ATCTAGACAG CCTAGTATGA ACCGTGGAGG GTACGTGCCC 
ACCCCTGCAG GGCCAAAGGT GGACTATATT CAGGGAGCAC CAGTGAGTGC TCACCTACAG 
CCTTCCCTCT CCAGACAGAG CAGCTACACC AGTAATGGCA CCCTTCCCCG GACGGGACTA 
AAGAGGACAC CGTCCTTAAA ACCTGACGTG CCGCCAAAGC CTTCATTTGT TCCTCAAACC 
ACCTCCGTCA GACCACTGAA CAAATACACT TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211871.1
CDS	345..3380
Translation	MRFFLLCAYMLLLLISQLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPKTEVIPNKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYKLNTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDETLSFIKSHPLMDSAVPPIADEPWFTKTRIRYRLTAIAVDHSAGPHQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNHAKCSAENEEDRKVISLQLDKDHHALYVAFSSCVIRIPLSRCERYGSCKKSCIASRDPYCGWLSQGACGRVSPAMLLLTEDLFAFHNHSAGGFEQDTEYGNTAHLGDCHGVRWEVQSGEANQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNGDTKSMVMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHGHGASRKETPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNIDHPLTKSSSKRDHRRSVDSRNTLNDLLKHLNDPNSNPKAIMGDIQMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAMPKNLSSPNGVLLSRQPSMNRGGYVPTPAGPKVDYIQGAPVSAHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_005211814.3
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 6D (SEMA6D), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211814.3

ORF Insert Sequence:

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC 
CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC 
TGCCACGGTG TACGGTGGGA AGTCCAGTCT GGAGAGGCCA ACCAGATGGT CCACATGAAT 
GTCCTCATCA CCTGTGTCTT TGCAGCTTTT GTCTTGGGTG CGTTCATTGC AGGTGTGGCA 
GTCTACTGTT ACCGTGACAT GTTTGTTCGG AAAAACAGAA AGATCCATAA AGATGCAGAA 
TCTGCCCAGT CATGCACAGA CTCCAGTGGA AGTTTTGCCA AGCTGAATGG TCTCTTTGAC 
AGCCCCGTCA AGGAATACCA ACAGAATATT GATTCTCCCA AATTATATAG TAACCTGCTG 
ACCAGTCGGA AAGAGCTGCC ACCCAATGGA GATACAAAAT CCATGGTCAT GGACCATCGA 
GGCCAACCTC CTGAGTTGGC TGCTCTCCCC ACTCCTGAGT CTACGCCTGT GCTTCACCAG 
AAGACCCTGC AGGCCATGAA GAGCCACTCA GAAAAGGCCC ATGGCCATGG AGCTTCAAGG 
AAAGAAACCC CCCAGTTTTT TCCTTCTAGT CCTCCACCAC ATTCCCCACT AAGTCACGGG 
CATATCCCCA GCGCCATTGT TCTTCCTAAT GCTACCCATG ACTACAACAC TTCTTTCTCA 
AACTCCAATG CTCACAAAGC TGAAAAGAAG CTTCAAAACA TTGACCATCC TCTTACAAAG 
TCATCCAGTA AAAGAGATCA CCGGCGTTCT GTGGATTCCA GAAATACCCT CAATGATCTC 
CTGAAGCATC TAAATGACCC AAACAGTAAC CCCAAAGCCA TCATGGGAGA CATCCAGATG 
GCCCACCAGA CCCTAATGCT GGATCCCGTG GGACCTATGT CTGAAGTCCC GCCCAAGGTC 
CCTAACCGCG AGGCATCTCT CTACTCTCCT CCCTCGACTC TTCCCAGAAA TAGCCCAACC 
AAGCGAGTGG ATGTTCCCAC CACTCCTGGA GTTCCGATGA CTTCTTTGGA AAGACAGAGG 
GGTTACCATA AAAATTCCTC CCAGAGGCAC TCTATATCGG CTATGCCTAA AAACTTAAGT 
TCACCAAATG GTGTTTTGTT ATCTAGACAG CCTAGTATGA ACCGTGGAGG GTACGTGCCC 
ACCCCTGCAG GGCCAAAGGT GGACTATATT CAGGGAGCAC CAGTGAGTGC TCACCTACAG 
CCTTCCCTCT CCAGACAGAG CAGCTACACC AGTAATGGCA CCCTTCCCCG GACGGGACTA 
AAGAGGACAC CGTCCTTAAA ACCTGACGTG CCGCCAAAGC CTTCATTTGT TCCTCAAACC 
ACCTCCGTCA GACCACTGAA CAAATACACT TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA6D ( XM_024997535.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853303.1 Bos taurus semaphorin 6D (SEMA6D), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa49888
Clone ID Related Accession (Same CDS sequence)	XM_024997535.1 , XM_024997534.1 , XM_010809338.3 , XM_010809338.2 , XM_024997536.1
Accession Version	XM_024997535.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	semaphorin-6D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC 
CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC 
TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA 
CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA GGCCAACCAG ATGGTCCACA TGAATGTCCT CATCACCTGT 
GTCTTTGCAG CTTTTGTCTT GGGTGCGTTC ATTGCAGGTG TGGCAGTCTA CTGTTACCGT 
GACATGTTTG TTCGGAAAAA CAGAAAGATC CATAAAGATG CAGAATCTGC CCAGTCATGC 
ACAGACTCCA GTGGAAGTTT TGCCAAGCTG AATGGTCTCT TTGACAGCCC CGTCAAGGAA 
TACCAACAGA ATATTGATTC TCCCAAATTA TATAGTAACC TGCTGACCAG TCGGAAAGAG 
CTGCCACCCA ATGGAGATAC AAAATCCATG GTCATGGACC ATCGAGGCCA ACCTCCTGAG 
TTGGCTGCTC TCCCCACTCC TGAGTCTACG CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCAGAAAA GGCCCATGGC CATGGAGCTT CAAGGAAAGA AACCCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCACATTCC CCACTAAGTC ACGGGCATAT CCCCAGCGCC 
ATTGTTCTTC CTAATGCTAC CCATGACTAC AACACTTCTT TCTCAAACTC CAATGCTCAC 
AAAGCTGAAA AGAAGCTTCA AAACATTGAC CATCCTCTTA CAAAGTCATC CAGTAAAAGA 
GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCATG GGAGACATCC AGATGGCCCA CCAGACCCTA 
ATGCTGGATC CCGTGGGACC TATGTCTGAA GTCCCGCCCA AGGTCCCTAA CCGCGAGGCA 
TCTCTCTACT CTCCTCCCTC GACTCTTCCC AGAAATAGCC CAACCAAGCG AGTGGATGTT 
CCCACCACTC CTGGAGTTCC GATGACTTCT TTGGAAAGAC AGAGGGGTTA CCATAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCGGCTATG CCTAAAAACT TAAGTTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCTAG TATGAACCGT GGAGGGTACG TGCCCACCCC TGCAGGGCCA 
AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGCTCACC TACAGCCTTC CCTCTCCAGA 
CAGAGCAGCT ACACCAGTAA TGGCACCCTT CCCCGGACGG GACTAAAGAG GACACCGTCC 
TTAAAACCTG ACGTGCCGCC AAAGCCTTCA TTTGTTCCTC AAACCACCTC CGTCAGACCA 
CTGAACAAAT ACACTTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853303.1
CDS	135..3395
Translation	MRFFLLCAYMLLLLISQLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPKTEVIPNKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYKLNTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDETLSFIKSHPLMDSAVPPIADEPWFTKTRIRYRLTAIAVDHSAGPHQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNHAKCSAENEEDRKVISLQLDKDHHALYVAFSSCVIRIPLSRCERYGSCKKSCIASRDPYCGWLSQGACGRVSPAMLLLTEDLFAFHNHSAGGFEQDTEYGNTAHLGDCHEILPTSTTPDYKIFGGPTSDMEVSSSSVTTMASIPEITPKVIDTWRPKLTSSRKFVVQDDPNTSDFTDPLSGIPKGVRWEVQSGEANQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNGDTKSMVMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHGHGASRKETPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNIDHPLTKSSSKRDHRRSVDSRNTLNDLLKHLNDPNSNPKAIMGDIQMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAMPKNLSSPNGVLLSRQPSMNRGGYVPTPAGPKVDYIQGAPVSAHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_024997535.1
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 6D (SEMA6D), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997535.1

ORF Insert Sequence:

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC 
CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC 
TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA 
CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA GGCCAACCAG ATGGTCCACA TGAATGTCCT CATCACCTGT 
GTCTTTGCAG CTTTTGTCTT GGGTGCGTTC ATTGCAGGTG TGGCAGTCTA CTGTTACCGT 
GACATGTTTG TTCGGAAAAA CAGAAAGATC CATAAAGATG CAGAATCTGC CCAGTCATGC 
ACAGACTCCA GTGGAAGTTT TGCCAAGCTG AATGGTCTCT TTGACAGCCC CGTCAAGGAA 
TACCAACAGA ATATTGATTC TCCCAAATTA TATAGTAACC TGCTGACCAG TCGGAAAGAG 
CTGCCACCCA ATGGAGATAC AAAATCCATG GTCATGGACC ATCGAGGCCA ACCTCCTGAG 
TTGGCTGCTC TCCCCACTCC TGAGTCTACG CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCAGAAAA GGCCCATGGC CATGGAGCTT CAAGGAAAGA AACCCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCACATTCC CCACTAAGTC ACGGGCATAT CCCCAGCGCC 
ATTGTTCTTC CTAATGCTAC CCATGACTAC AACACTTCTT TCTCAAACTC CAATGCTCAC 
AAAGCTGAAA AGAAGCTTCA AAACATTGAC CATCCTCTTA CAAAGTCATC CAGTAAAAGA 
GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCATG GGAGACATCC AGATGGCCCA CCAGACCCTA 
ATGCTGGATC CCGTGGGACC TATGTCTGAA GTCCCGCCCA AGGTCCCTAA CCGCGAGGCA 
TCTCTCTACT CTCCTCCCTC GACTCTTCCC AGAAATAGCC CAACCAAGCG AGTGGATGTT 
CCCACCACTC CTGGAGTTCC GATGACTTCT TTGGAAAGAC AGAGGGGTTA CCATAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCGGCTATG CCTAAAAACT TAAGTTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCTAG TATGAACCGT GGAGGGTACG TGCCCACCCC TGCAGGGCCA 
AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGCTCACC TACAGCCTTC CCTCTCCAGA 
CAGAGCAGCT ACACCAGTAA TGGCACCCTT CCCCGGACGG GACTAAAGAG GACACCGTCC 
TTAAAACCTG ACGTGCCGCC AAAGCCTTCA TTTGTTCCTC AAACCACCTC CGTCAGACCA 
CTGAACAAAT ACACTTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA6D ( XM_024997534.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853302.1 Bos taurus semaphorin 6D (SEMA6D), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa49888
Clone ID Related Accession (Same CDS sequence)	XM_024997535.1 , XM_024997534.1 , XM_010809338.3 , XM_010809338.2 , XM_024997536.1
Accession Version	XM_024997534.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	semaphorin-6D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC 
CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC 
TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA 
CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA GGCCAACCAG ATGGTCCACA TGAATGTCCT CATCACCTGT 
GTCTTTGCAG CTTTTGTCTT GGGTGCGTTC ATTGCAGGTG TGGCAGTCTA CTGTTACCGT 
GACATGTTTG TTCGGAAAAA CAGAAAGATC CATAAAGATG CAGAATCTGC CCAGTCATGC 
ACAGACTCCA GTGGAAGTTT TGCCAAGCTG AATGGTCTCT TTGACAGCCC CGTCAAGGAA 
TACCAACAGA ATATTGATTC TCCCAAATTA TATAGTAACC TGCTGACCAG TCGGAAAGAG 
CTGCCACCCA ATGGAGATAC AAAATCCATG GTCATGGACC ATCGAGGCCA ACCTCCTGAG 
TTGGCTGCTC TCCCCACTCC TGAGTCTACG CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCAGAAAA GGCCCATGGC CATGGAGCTT CAAGGAAAGA AACCCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCACATTCC CCACTAAGTC ACGGGCATAT CCCCAGCGCC 
ATTGTTCTTC CTAATGCTAC CCATGACTAC AACACTTCTT TCTCAAACTC CAATGCTCAC 
AAAGCTGAAA AGAAGCTTCA AAACATTGAC CATCCTCTTA CAAAGTCATC CAGTAAAAGA 
GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCATG GGAGACATCC AGATGGCCCA CCAGACCCTA 
ATGCTGGATC CCGTGGGACC TATGTCTGAA GTCCCGCCCA AGGTCCCTAA CCGCGAGGCA 
TCTCTCTACT CTCCTCCCTC GACTCTTCCC AGAAATAGCC CAACCAAGCG AGTGGATGTT 
CCCACCACTC CTGGAGTTCC GATGACTTCT TTGGAAAGAC AGAGGGGTTA CCATAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCGGCTATG CCTAAAAACT TAAGTTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCTAG TATGAACCGT GGAGGGTACG TGCCCACCCC TGCAGGGCCA 
AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGCTCACC TACAGCCTTC CCTCTCCAGA 
CAGAGCAGCT ACACCAGTAA TGGCACCCTT CCCCGGACGG GACTAAAGAG GACACCGTCC 
TTAAAACCTG ACGTGCCGCC AAAGCCTTCA TTTGTTCCTC AAACCACCTC CGTCAGACCA 
CTGAACAAAT ACACTTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853302.1
CDS	134..3394
Translation	MRFFLLCAYMLLLLISQLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPKTEVIPNKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYKLNTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDETLSFIKSHPLMDSAVPPIADEPWFTKTRIRYRLTAIAVDHSAGPHQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNHAKCSAENEEDRKVISLQLDKDHHALYVAFSSCVIRIPLSRCERYGSCKKSCIASRDPYCGWLSQGACGRVSPAMLLLTEDLFAFHNHSAGGFEQDTEYGNTAHLGDCHEILPTSTTPDYKIFGGPTSDMEVSSSSVTTMASIPEITPKVIDTWRPKLTSSRKFVVQDDPNTSDFTDPLSGIPKGVRWEVQSGEANQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNGDTKSMVMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHGHGASRKETPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNIDHPLTKSSSKRDHRRSVDSRNTLNDLLKHLNDPNSNPKAIMGDIQMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAMPKNLSSPNGVLLSRQPSMNRGGYVPTPAGPKVDYIQGAPVSAHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_024997534.1
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 6D (SEMA6D), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997534.1

ORF Insert Sequence:

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC 
CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC 
TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA 
CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA GGCCAACCAG ATGGTCCACA TGAATGTCCT CATCACCTGT 
GTCTTTGCAG CTTTTGTCTT GGGTGCGTTC ATTGCAGGTG TGGCAGTCTA CTGTTACCGT 
GACATGTTTG TTCGGAAAAA CAGAAAGATC CATAAAGATG CAGAATCTGC CCAGTCATGC 
ACAGACTCCA GTGGAAGTTT TGCCAAGCTG AATGGTCTCT TTGACAGCCC CGTCAAGGAA 
TACCAACAGA ATATTGATTC TCCCAAATTA TATAGTAACC TGCTGACCAG TCGGAAAGAG 
CTGCCACCCA ATGGAGATAC AAAATCCATG GTCATGGACC ATCGAGGCCA ACCTCCTGAG 
TTGGCTGCTC TCCCCACTCC TGAGTCTACG CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCAGAAAA GGCCCATGGC CATGGAGCTT CAAGGAAAGA AACCCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCACATTCC CCACTAAGTC ACGGGCATAT CCCCAGCGCC 
ATTGTTCTTC CTAATGCTAC CCATGACTAC AACACTTCTT TCTCAAACTC CAATGCTCAC 
AAAGCTGAAA AGAAGCTTCA AAACATTGAC CATCCTCTTA CAAAGTCATC CAGTAAAAGA 
GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCATG GGAGACATCC AGATGGCCCA CCAGACCCTA 
ATGCTGGATC CCGTGGGACC TATGTCTGAA GTCCCGCCCA AGGTCCCTAA CCGCGAGGCA 
TCTCTCTACT CTCCTCCCTC GACTCTTCCC AGAAATAGCC CAACCAAGCG AGTGGATGTT 
CCCACCACTC CTGGAGTTCC GATGACTTCT TTGGAAAGAC AGAGGGGTTA CCATAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCGGCTATG CCTAAAAACT TAAGTTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCTAG TATGAACCGT GGAGGGTACG TGCCCACCCC TGCAGGGCCA 
AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGCTCACC TACAGCCTTC CCTCTCCAGA 
CAGAGCAGCT ACACCAGTAA TGGCACCCTT CCCCGGACGG GACTAAAGAG GACACCGTCC 
TTAAAACCTG ACGTGCCGCC AAAGCCTTCA TTTGTTCCTC AAACCACCTC CGTCAGACCA 
CTGAACAAAT ACACTTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA6D ( XM_010809338.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807640.1 Bos taurus semaphorin 6D (SEMA6D), transcript variant X1, mRNA.

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CloneID	OBa49888
Clone ID Related Accession (Same CDS sequence)	XM_024997535.1 , XM_024997534.1 , XM_010809338.3 , XM_010809338.2 , XM_024997536.1
Accession Version	XM_010809338.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	semaphorin-6D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809338.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC 
CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC 
TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA 
CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA GGCCAACCAG ATGGTCCACA TGAATGTCCT CATCACCTGT 
GTCTTTGCAG CTTTTGTCTT GGGTGCGTTC ATTGCAGGTG TGGCAGTCTA CTGTTACCGT 
GACATGTTTG TTCGGAAAAA CAGAAAGATC CATAAAGATG CAGAATCTGC CCAGTCATGC 
ACAGACTCCA GTGGAAGTTT TGCCAAGCTG AATGGTCTCT TTGACAGCCC CGTCAAGGAA 
TACCAACAGA ATATTGATTC TCCCAAATTA TATAGTAACC TGCTGACCAG TCGGAAAGAG 
CTGCCACCCA ATGGAGATAC AAAATCCATG GTCATGGACC ATCGAGGCCA ACCTCCTGAG 
TTGGCTGCTC TCCCCACTCC TGAGTCTACG CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCAGAAAA GGCCCATGGC CATGGAGCTT CAAGGAAAGA AACCCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCACATTCC CCACTAAGTC ACGGGCATAT CCCCAGCGCC 
ATTGTTCTTC CTAATGCTAC CCATGACTAC AACACTTCTT TCTCAAACTC CAATGCTCAC 
AAAGCTGAAA AGAAGCTTCA AAACATTGAC CATCCTCTTA CAAAGTCATC CAGTAAAAGA 
GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCATG GGAGACATCC AGATGGCCCA CCAGACCCTA 
ATGCTGGATC CCGTGGGACC TATGTCTGAA GTCCCGCCCA AGGTCCCTAA CCGCGAGGCA 
TCTCTCTACT CTCCTCCCTC GACTCTTCCC AGAAATAGCC CAACCAAGCG AGTGGATGTT 
CCCACCACTC CTGGAGTTCC GATGACTTCT TTGGAAAGAC AGAGGGGTTA CCATAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCGGCTATG CCTAAAAACT TAAGTTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCTAG TATGAACCGT GGAGGGTACG TGCCCACCCC TGCAGGGCCA 
AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGCTCACC TACAGCCTTC CCTCTCCAGA 
CAGAGCAGCT ACACCAGTAA TGGCACCCTT CCCCGGACGG GACTAAAGAG GACACCGTCC 
TTAAAACCTG ACGTGCCGCC AAAGCCTTCA TTTGTTCCTC AAACCACCTC CGTCAGACCA 
CTGAACAAAT ACACTTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807640.1
CDS	180..3440
Translation	MRFFLLCAYMLLLLISQLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPKTEVIPNKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYKLNTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDETLSFIKSHPLMDSAVPPIADEPWFTKTRIRYRLTAIAVDHSAGPHQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNHAKCSAENEEDRKVISLQLDKDHHALYVAFSSCVIRIPLSRCERYGSCKKSCIASRDPYCGWLSQGACGRVSPAMLLLTEDLFAFHNHSAGGFEQDTEYGNTAHLGDCHEILPTSTTPDYKIFGGPTSDMEVSSSSVTTMASIPEITPKVIDTWRPKLTSSRKFVVQDDPNTSDFTDPLSGIPKGVRWEVQSGEANQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNGDTKSMVMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHGHGASRKETPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNIDHPLTKSSSKRDHRRSVDSRNTLNDLLKHLNDPNSNPKAIMGDIQMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAMPKNLSSPNGVLLSRQPSMNRGGYVPTPAGPKVDYIQGAPVSAHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_010809338.3
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 6D (SEMA6D), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809338.3

ORF Insert Sequence:

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC 
CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC 
TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA 
CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA GGCCAACCAG ATGGTCCACA TGAATGTCCT CATCACCTGT 
GTCTTTGCAG CTTTTGTCTT GGGTGCGTTC ATTGCAGGTG TGGCAGTCTA CTGTTACCGT 
GACATGTTTG TTCGGAAAAA CAGAAAGATC CATAAAGATG CAGAATCTGC CCAGTCATGC 
ACAGACTCCA GTGGAAGTTT TGCCAAGCTG AATGGTCTCT TTGACAGCCC CGTCAAGGAA 
TACCAACAGA ATATTGATTC TCCCAAATTA TATAGTAACC TGCTGACCAG TCGGAAAGAG 
CTGCCACCCA ATGGAGATAC AAAATCCATG GTCATGGACC ATCGAGGCCA ACCTCCTGAG 
TTGGCTGCTC TCCCCACTCC TGAGTCTACG CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCAGAAAA GGCCCATGGC CATGGAGCTT CAAGGAAAGA AACCCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCACATTCC CCACTAAGTC ACGGGCATAT CCCCAGCGCC 
ATTGTTCTTC CTAATGCTAC CCATGACTAC AACACTTCTT TCTCAAACTC CAATGCTCAC 
AAAGCTGAAA AGAAGCTTCA AAACATTGAC CATCCTCTTA CAAAGTCATC CAGTAAAAGA 
GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCATG GGAGACATCC AGATGGCCCA CCAGACCCTA 
ATGCTGGATC CCGTGGGACC TATGTCTGAA GTCCCGCCCA AGGTCCCTAA CCGCGAGGCA 
TCTCTCTACT CTCCTCCCTC GACTCTTCCC AGAAATAGCC CAACCAAGCG AGTGGATGTT 
CCCACCACTC CTGGAGTTCC GATGACTTCT TTGGAAAGAC AGAGGGGTTA CCATAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCGGCTATG CCTAAAAACT TAAGTTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCTAG TATGAACCGT GGAGGGTACG TGCCCACCCC TGCAGGGCCA 
AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGCTCACC TACAGCCTTC CCTCTCCAGA 
CAGAGCAGCT ACACCAGTAA TGGCACCCTT CCCCGGACGG GACTAAAGAG GACACCGTCC 
TTAAAACCTG ACGTGCCGCC AAAGCCTTCA TTTGTTCCTC AAACCACCTC CGTCAGACCA 
CTGAACAAAT ACACTTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA6D ( XM_010809338.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807640.1 Bos taurus semaphorin 6D (SEMA6D), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa49888
Clone ID Related Accession (Same CDS sequence)	XM_024997535.1 , XM_024997534.1 , XM_010809338.3 , XM_010809338.2 , XM_024997536.1
Accession Version	XM_010809338.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	semaphorin-6D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809338.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC 
CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC 
TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA 
CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA GGCCAACCAG ATGGTCCACA TGAATGTCCT CATCACCTGT 
GTCTTTGCAG CTTTTGTCTT GGGTGCGTTC ATTGCAGGTG TGGCAGTCTA CTGTTACCGT 
GACATGTTTG TTCGGAAAAA CAGAAAGATC CATAAAGATG CAGAATCTGC CCAGTCATGC 
ACAGACTCCA GTGGAAGTTT TGCCAAGCTG AATGGTCTCT TTGACAGCCC CGTCAAGGAA 
TACCAACAGA ATATTGATTC TCCCAAATTA TATAGTAACC TGCTGACCAG TCGGAAAGAG 
CTGCCACCCA ATGGAGATAC AAAATCCATG GTCATGGACC ATCGAGGCCA ACCTCCTGAG 
TTGGCTGCTC TCCCCACTCC TGAGTCTACG CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCAGAAAA GGCCCATGGC CATGGAGCTT CAAGGAAAGA AACCCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCACATTCC CCACTAAGTC ACGGGCATAT CCCCAGCGCC 
ATTGTTCTTC CTAATGCTAC CCATGACTAC AACACTTCTT TCTCAAACTC CAATGCTCAC 
AAAGCTGAAA AGAAGCTTCA AAACATTGAC CATCCTCTTA CAAAGTCATC CAGTAAAAGA 
GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCATG GGAGACATCC AGATGGCCCA CCAGACCCTA 
ATGCTGGATC CCGTGGGACC TATGTCTGAA GTCCCGCCCA AGGTCCCTAA CCGCGAGGCA 
TCTCTCTACT CTCCTCCCTC GACTCTTCCC AGAAATAGCC CAACCAAGCG AGTGGATGTT 
CCCACCACTC CTGGAGTTCC GATGACTTCT TTGGAAAGAC AGAGGGGTTA CCATAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCGGCTATG CCTAAAAACT TAAGTTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCTAG TATGAACCGT GGAGGGTACG TGCCCACCCC TGCAGGGCCA 
AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGCTCACC TACAGCCTTC CCTCTCCAGA 
CAGAGCAGCT ACACCAGTAA TGGCACCCTT CCCCGGACGG GACTAAAGAG GACACCGTCC 
TTAAAACCTG ACGTGCCGCC AAAGCCTTCA TTTGTTCCTC AAACCACCTC CGTCAGACCA 
CTGAACAAAT ACACTTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807640.1
CDS	345..3605
Translation	MRFFLLCAYMLLLLISQLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPKTEVIPNKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYKLNTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDETLSFIKSHPLMDSAVPPIADEPWFTKTRIRYRLTAIAVDHSAGPHQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNHAKCSAENEEDRKVISLQLDKDHHALYVAFSSCVIRIPLSRCERYGSCKKSCIASRDPYCGWLSQGACGRVSPAMLLLTEDLFAFHNHSAGGFEQDTEYGNTAHLGDCHEILPTSTTPDYKIFGGPTSDMEVSSSSVTTMASIPEITPKVIDTWRPKLTSSRKFVVQDDPNTSDFTDPLSGIPKGVRWEVQSGEANQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNGDTKSMVMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHGHGASRKETPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNIDHPLTKSSSKRDHRRSVDSRNTLNDLLKHLNDPNSNPKAIMGDIQMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAMPKNLSSPNGVLLSRQPSMNRGGYVPTPAGPKVDYIQGAPVSAHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_010809338.2
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 6D (SEMA6D), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809338.2

ORF Insert Sequence:

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC 
CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC 
TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA 
CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA GGCCAACCAG ATGGTCCACA TGAATGTCCT CATCACCTGT 
GTCTTTGCAG CTTTTGTCTT GGGTGCGTTC ATTGCAGGTG TGGCAGTCTA CTGTTACCGT 
GACATGTTTG TTCGGAAAAA CAGAAAGATC CATAAAGATG CAGAATCTGC CCAGTCATGC 
ACAGACTCCA GTGGAAGTTT TGCCAAGCTG AATGGTCTCT TTGACAGCCC CGTCAAGGAA 
TACCAACAGA ATATTGATTC TCCCAAATTA TATAGTAACC TGCTGACCAG TCGGAAAGAG 
CTGCCACCCA ATGGAGATAC AAAATCCATG GTCATGGACC ATCGAGGCCA ACCTCCTGAG 
TTGGCTGCTC TCCCCACTCC TGAGTCTACG CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCAGAAAA GGCCCATGGC CATGGAGCTT CAAGGAAAGA AACCCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCACATTCC CCACTAAGTC ACGGGCATAT CCCCAGCGCC 
ATTGTTCTTC CTAATGCTAC CCATGACTAC AACACTTCTT TCTCAAACTC CAATGCTCAC 
AAAGCTGAAA AGAAGCTTCA AAACATTGAC CATCCTCTTA CAAAGTCATC CAGTAAAAGA 
GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCATG GGAGACATCC AGATGGCCCA CCAGACCCTA 
ATGCTGGATC CCGTGGGACC TATGTCTGAA GTCCCGCCCA AGGTCCCTAA CCGCGAGGCA 
TCTCTCTACT CTCCTCCCTC GACTCTTCCC AGAAATAGCC CAACCAAGCG AGTGGATGTT 
CCCACCACTC CTGGAGTTCC GATGACTTCT TTGGAAAGAC AGAGGGGTTA CCATAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCGGCTATG CCTAAAAACT TAAGTTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCTAG TATGAACCGT GGAGGGTACG TGCCCACCCC TGCAGGGCCA 
AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGCTCACC TACAGCCTTC CCTCTCCAGA 
CAGAGCAGCT ACACCAGTAA TGGCACCCTT CCCCGGACGG GACTAAAGAG GACACCGTCC 
TTAAAACCTG ACGTGCCGCC AAAGCCTTCA TTTGTTCCTC AAACCACCTC CGTCAGACCA 
CTGAACAAAT ACACTTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA6D ( XM_024997536.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853304.1 Bos taurus semaphorin 6D (SEMA6D), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa49888
Clone ID Related Accession (Same CDS sequence)	XM_024997535.1 , XM_024997534.1 , XM_010809338.3 , XM_010809338.2 , XM_024997536.1
Accession Version	XM_024997536.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	semaphorin-6D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC 
CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC 
TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA 
CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA GGCCAACCAG ATGGTCCACA TGAATGTCCT CATCACCTGT 
GTCTTTGCAG CTTTTGTCTT GGGTGCGTTC ATTGCAGGTG TGGCAGTCTA CTGTTACCGT 
GACATGTTTG TTCGGAAAAA CAGAAAGATC CATAAAGATG CAGAATCTGC CCAGTCATGC 
ACAGACTCCA GTGGAAGTTT TGCCAAGCTG AATGGTCTCT TTGACAGCCC CGTCAAGGAA 
TACCAACAGA ATATTGATTC TCCCAAATTA TATAGTAACC TGCTGACCAG TCGGAAAGAG 
CTGCCACCCA ATGGAGATAC AAAATCCATG GTCATGGACC ATCGAGGCCA ACCTCCTGAG 
TTGGCTGCTC TCCCCACTCC TGAGTCTACG CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCAGAAAA GGCCCATGGC CATGGAGCTT CAAGGAAAGA AACCCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCACATTCC CCACTAAGTC ACGGGCATAT CCCCAGCGCC 
ATTGTTCTTC CTAATGCTAC CCATGACTAC AACACTTCTT TCTCAAACTC CAATGCTCAC 
AAAGCTGAAA AGAAGCTTCA AAACATTGAC CATCCTCTTA CAAAGTCATC CAGTAAAAGA 
GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCATG GGAGACATCC AGATGGCCCA CCAGACCCTA 
ATGCTGGATC CCGTGGGACC TATGTCTGAA GTCCCGCCCA AGGTCCCTAA CCGCGAGGCA 
TCTCTCTACT CTCCTCCCTC GACTCTTCCC AGAAATAGCC CAACCAAGCG AGTGGATGTT 
CCCACCACTC CTGGAGTTCC GATGACTTCT TTGGAAAGAC AGAGGGGTTA CCATAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCGGCTATG CCTAAAAACT TAAGTTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCTAG TATGAACCGT GGAGGGTACG TGCCCACCCC TGCAGGGCCA 
AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGCTCACC TACAGCCTTC CCTCTCCAGA 
CAGAGCAGCT ACACCAGTAA TGGCACCCTT CCCCGGACGG GACTAAAGAG GACACCGTCC 
TTAAAACCTG ACGTGCCGCC AAAGCCTTCA TTTGTTCCTC AAACCACCTC CGTCAGACCA 
CTGAACAAAT ACACTTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853304.1
CDS	939..4199
Translation	MRFFLLCAYMLLLLISQLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPKTEVIPNKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYKLNTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDETLSFIKSHPLMDSAVPPIADEPWFTKTRIRYRLTAIAVDHSAGPHQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNHAKCSAENEEDRKVISLQLDKDHHALYVAFSSCVIRIPLSRCERYGSCKKSCIASRDPYCGWLSQGACGRVSPAMLLLTEDLFAFHNHSAGGFEQDTEYGNTAHLGDCHEILPTSTTPDYKIFGGPTSDMEVSSSSVTTMASIPEITPKVIDTWRPKLTSSRKFVVQDDPNTSDFTDPLSGIPKGVRWEVQSGEANQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNGDTKSMVMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHGHGASRKETPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNIDHPLTKSSSKRDHRRSVDSRNTLNDLLKHLNDPNSNPKAIMGDIQMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAMPKNLSSPNGVLLSRQPSMNRGGYVPTPAGPKVDYIQGAPVSAHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_024997536.1
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 6D (SEMA6D), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997536.1

ORF Insert Sequence:

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC 
CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC 
TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA 
CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTATCCCAAA GGGTGTACGG 
TGGGAAGTCC AGTCTGGAGA GGCCAACCAG ATGGTCCACA TGAATGTCCT CATCACCTGT 
GTCTTTGCAG CTTTTGTCTT GGGTGCGTTC ATTGCAGGTG TGGCAGTCTA CTGTTACCGT 
GACATGTTTG TTCGGAAAAA CAGAAAGATC CATAAAGATG CAGAATCTGC CCAGTCATGC 
ACAGACTCCA GTGGAAGTTT TGCCAAGCTG AATGGTCTCT TTGACAGCCC CGTCAAGGAA 
TACCAACAGA ATATTGATTC TCCCAAATTA TATAGTAACC TGCTGACCAG TCGGAAAGAG 
CTGCCACCCA ATGGAGATAC AAAATCCATG GTCATGGACC ATCGAGGCCA ACCTCCTGAG 
TTGGCTGCTC TCCCCACTCC TGAGTCTACG CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC 
ATGAAGAGCC ACTCAGAAAA GGCCCATGGC CATGGAGCTT CAAGGAAAGA AACCCCCCAG 
TTTTTTCCTT CTAGTCCTCC ACCACATTCC CCACTAAGTC ACGGGCATAT CCCCAGCGCC 
ATTGTTCTTC CTAATGCTAC CCATGACTAC AACACTTCTT TCTCAAACTC CAATGCTCAC 
AAAGCTGAAA AGAAGCTTCA AAACATTGAC CATCCTCTTA CAAAGTCATC CAGTAAAAGA 
GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATCTAAAT 
GACCCAAACA GTAACCCCAA AGCCATCATG GGAGACATCC AGATGGCCCA CCAGACCCTA 
ATGCTGGATC CCGTGGGACC TATGTCTGAA GTCCCGCCCA AGGTCCCTAA CCGCGAGGCA 
TCTCTCTACT CTCCTCCCTC GACTCTTCCC AGAAATAGCC CAACCAAGCG AGTGGATGTT 
CCCACCACTC CTGGAGTTCC GATGACTTCT TTGGAAAGAC AGAGGGGTTA CCATAAAAAT 
TCCTCCCAGA GGCACTCTAT ATCGGCTATG CCTAAAAACT TAAGTTCACC AAATGGTGTT 
TTGTTATCTA GACAGCCTAG TATGAACCGT GGAGGGTACG TGCCCACCCC TGCAGGGCCA 
AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGCTCACC TACAGCCTTC CCTCTCCAGA 
CAGAGCAGCT ACACCAGTAA TGGCACCCTT CCCCGGACGG GACTAAAGAG GACACCGTCC 
TTAAAACCTG ACGTGCCGCC AAAGCCTTCA TTTGTTCCTC AAACCACCTC CGTCAGACCA 
CTGAACAAAT ACACTTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA6D ( XM_010809340.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807642.1 Bos taurus semaphorin 6D (SEMA6D), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa49889
Clone ID Related Accession (Same CDS sequence)	XM_010809340.3 , XM_010809340.2
Accession Version	XM_010809340.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	semaphorin-6D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809340.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC 
CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC 
TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA 
CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTGTACGGTG GGAAGTCCAG 
TCTGGAGAGG CCAACCAGAT GGTCCACATG AATGTCCTCA TCACCTGTGT CTTTGCAGCT 
TTTGTCTTGG GTGCGTTCAT TGCAGGTGTG GCAGTCTACT GTTACCGTGA CATGTTTGTT 
CGGAAAAACA GAAAGATCCA TAAAGATGCA GAATCTGCCC AGTCATGCAC AGACTCCAGT 
GGAAGTTTTG CCAAGCTGAA TGGTCTCTTT GACAGCCCCG TCAAGGAATA CCAACAGAAT 
ATTGATTCTC CCAAATTATA TAGTAACCTG CTGACCAGTC GGAAAGAGCT GCCACCCAAT 
GGAGATACAA AATCCATGGT CATGGACCAT CGAGGCCAAC CTCCTGAGTT GGCTGCTCTC 
CCCACTCCTG AGTCTACGCC TGTGCTTCAC CAGAAGACCC TGCAGGCCAT GAAGAGCCAC 
TCAGAAAAGG CCCATGGCCA TGGAGCTTCA AGGAAAGAAA CCCCCCAGTT TTTTCCTTCT 
AGTCCTCCAC CACATTCCCC ACTAAGTCAC GGGCATATCC CCAGCGCCAT TGTTCTTCCT 
AATGCTACCC ATGACTACAA CACTTCTTTC TCAAACTCCA ATGCTCACAA AGCTGAAAAG 
AAGCTTCAAA ACATTGACCA TCCTCTTACA AAGTCATCCA GTAAAAGAGA TCACCGGCGT 
TCTGTGGATT CCAGAAATAC CCTCAATGAT CTCCTGAAGC ATCTAAATGA CCCAAACAGT 
AACCCCAAAG CCATCATGGG AGACATCCAG ATGGCCCACC AGACCCTAAT GCTGGATCCC 
GTGGGACCTA TGTCTGAAGT CCCGCCCAAG GTCCCTAACC GCGAGGCATC TCTCTACTCT 
CCTCCCTCGA CTCTTCCCAG AAATAGCCCA ACCAAGCGAG TGGATGTTCC CACCACTCCT 
GGAGTTCCGA TGACTTCTTT GGAAAGACAG AGGGGTTACC ATAAAAATTC CTCCCAGAGG 
CACTCTATAT CGGCTATGCC TAAAAACTTA AGTTCACCAA ATGGTGTTTT GTTATCTAGA 
CAGCCTAGTA TGAACCGTGG AGGGTACGTG CCCACCCCTG CAGGGCCAAA GGTGGACTAT 
ATTCAGGGAG CACCAGTGAG TGCTCACCTA CAGCCTTCCC TCTCCAGACA GAGCAGCTAC 
ACCAGTAATG GCACCCTTCC CCGGACGGGA CTAAAGAGGA CACCGTCCTT AAAACCTGAC 
GTGCCGCCAA AGCCTTCATT TGTTCCTCAA ACCACCTCCG TCAGACCACT GAACAAATAC 
ACTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807642.1
CDS	180..3428
Translation	MRFFLLCAYMLLLLISQLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPKTEVIPNKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYKLNTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDETLSFIKSHPLMDSAVPPIADEPWFTKTRIRYRLTAIAVDHSAGPHQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNHAKCSAENEEDRKVISLQLDKDHHALYVAFSSCVIRIPLSRCERYGSCKKSCIASRDPYCGWLSQGACGRVSPAMLLLTEDLFAFHNHSAGGFEQDTEYGNTAHLGDCHEILPTSTTPDYKIFGGPTSDMEVSSSSVTTMASIPEITPKVIDTWRPKLTSSRKFVVQDDPNTSDFTDPLSGVRWEVQSGEANQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNGDTKSMVMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHGHGASRKETPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNIDHPLTKSSSKRDHRRSVDSRNTLNDLLKHLNDPNSNPKAIMGDIQMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAMPKNLSSPNGVLLSRQPSMNRGGYVPTPAGPKVDYIQGAPVSAHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_010809340.3
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 6D (SEMA6D), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809340.3

ORF Insert Sequence:

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC 
CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC 
TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA 
CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTGTACGGTG GGAAGTCCAG 
TCTGGAGAGG CCAACCAGAT GGTCCACATG AATGTCCTCA TCACCTGTGT CTTTGCAGCT 
TTTGTCTTGG GTGCGTTCAT TGCAGGTGTG GCAGTCTACT GTTACCGTGA CATGTTTGTT 
CGGAAAAACA GAAAGATCCA TAAAGATGCA GAATCTGCCC AGTCATGCAC AGACTCCAGT 
GGAAGTTTTG CCAAGCTGAA TGGTCTCTTT GACAGCCCCG TCAAGGAATA CCAACAGAAT 
ATTGATTCTC CCAAATTATA TAGTAACCTG CTGACCAGTC GGAAAGAGCT GCCACCCAAT 
GGAGATACAA AATCCATGGT CATGGACCAT CGAGGCCAAC CTCCTGAGTT GGCTGCTCTC 
CCCACTCCTG AGTCTACGCC TGTGCTTCAC CAGAAGACCC TGCAGGCCAT GAAGAGCCAC 
TCAGAAAAGG CCCATGGCCA TGGAGCTTCA AGGAAAGAAA CCCCCCAGTT TTTTCCTTCT 
AGTCCTCCAC CACATTCCCC ACTAAGTCAC GGGCATATCC CCAGCGCCAT TGTTCTTCCT 
AATGCTACCC ATGACTACAA CACTTCTTTC TCAAACTCCA ATGCTCACAA AGCTGAAAAG 
AAGCTTCAAA ACATTGACCA TCCTCTTACA AAGTCATCCA GTAAAAGAGA TCACCGGCGT 
TCTGTGGATT CCAGAAATAC CCTCAATGAT CTCCTGAAGC ATCTAAATGA CCCAAACAGT 
AACCCCAAAG CCATCATGGG AGACATCCAG ATGGCCCACC AGACCCTAAT GCTGGATCCC 
GTGGGACCTA TGTCTGAAGT CCCGCCCAAG GTCCCTAACC GCGAGGCATC TCTCTACTCT 
CCTCCCTCGA CTCTTCCCAG AAATAGCCCA ACCAAGCGAG TGGATGTTCC CACCACTCCT 
GGAGTTCCGA TGACTTCTTT GGAAAGACAG AGGGGTTACC ATAAAAATTC CTCCCAGAGG 
CACTCTATAT CGGCTATGCC TAAAAACTTA AGTTCACCAA ATGGTGTTTT GTTATCTAGA 
CAGCCTAGTA TGAACCGTGG AGGGTACGTG CCCACCCCTG CAGGGCCAAA GGTGGACTAT 
ATTCAGGGAG CACCAGTGAG TGCTCACCTA CAGCCTTCCC TCTCCAGACA GAGCAGCTAC 
ACCAGTAATG GCACCCTTCC CCGGACGGGA CTAAAGAGGA CACCGTCCTT AAAACCTGAC 
GTGCCGCCAA AGCCTTCATT TGTTCCTCAA ACCACCTCCG TCAGACCACT GAACAAATAC 
ACTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA6D ( XM_010809340.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807642.1 Bos taurus semaphorin 6D (SEMA6D), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa49889
Clone ID Related Accession (Same CDS sequence)	XM_010809340.3 , XM_010809340.2
Accession Version	XM_010809340.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	semaphorin-6D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809340.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC 
CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC 
TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA 
CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTGTACGGTG GGAAGTCCAG 
TCTGGAGAGG CCAACCAGAT GGTCCACATG AATGTCCTCA TCACCTGTGT CTTTGCAGCT 
TTTGTCTTGG GTGCGTTCAT TGCAGGTGTG GCAGTCTACT GTTACCGTGA CATGTTTGTT 
CGGAAAAACA GAAAGATCCA TAAAGATGCA GAATCTGCCC AGTCATGCAC AGACTCCAGT 
GGAAGTTTTG CCAAGCTGAA TGGTCTCTTT GACAGCCCCG TCAAGGAATA CCAACAGAAT 
ATTGATTCTC CCAAATTATA TAGTAACCTG CTGACCAGTC GGAAAGAGCT GCCACCCAAT 
GGAGATACAA AATCCATGGT CATGGACCAT CGAGGCCAAC CTCCTGAGTT GGCTGCTCTC 
CCCACTCCTG AGTCTACGCC TGTGCTTCAC CAGAAGACCC TGCAGGCCAT GAAGAGCCAC 
TCAGAAAAGG CCCATGGCCA TGGAGCTTCA AGGAAAGAAA CCCCCCAGTT TTTTCCTTCT 
AGTCCTCCAC CACATTCCCC ACTAAGTCAC GGGCATATCC CCAGCGCCAT TGTTCTTCCT 
AATGCTACCC ATGACTACAA CACTTCTTTC TCAAACTCCA ATGCTCACAA AGCTGAAAAG 
AAGCTTCAAA ACATTGACCA TCCTCTTACA AAGTCATCCA GTAAAAGAGA TCACCGGCGT 
TCTGTGGATT CCAGAAATAC CCTCAATGAT CTCCTGAAGC ATCTAAATGA CCCAAACAGT 
AACCCCAAAG CCATCATGGG AGACATCCAG ATGGCCCACC AGACCCTAAT GCTGGATCCC 
GTGGGACCTA TGTCTGAAGT CCCGCCCAAG GTCCCTAACC GCGAGGCATC TCTCTACTCT 
CCTCCCTCGA CTCTTCCCAG AAATAGCCCA ACCAAGCGAG TGGATGTTCC CACCACTCCT 
GGAGTTCCGA TGACTTCTTT GGAAAGACAG AGGGGTTACC ATAAAAATTC CTCCCAGAGG 
CACTCTATAT CGGCTATGCC TAAAAACTTA AGTTCACCAA ATGGTGTTTT GTTATCTAGA 
CAGCCTAGTA TGAACCGTGG AGGGTACGTG CCCACCCCTG CAGGGCCAAA GGTGGACTAT 
ATTCAGGGAG CACCAGTGAG TGCTCACCTA CAGCCTTCCC TCTCCAGACA GAGCAGCTAC 
ACCAGTAATG GCACCCTTCC CCGGACGGGA CTAAAGAGGA CACCGTCCTT AAAACCTGAC 
GTGCCGCCAA AGCCTTCATT TGTTCCTCAA ACCACCTCCG TCAGACCACT GAACAAATAC 
ACTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807642.1
CDS	345..3593
Translation	MRFFLLCAYMLLLLISQLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPKTEVIPNKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYKLNTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDETLSFIKSHPLMDSAVPPIADEPWFTKTRIRYRLTAIAVDHSAGPHQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNHAKCSAENEEDRKVISLQLDKDHHALYVAFSSCVIRIPLSRCERYGSCKKSCIASRDPYCGWLSQGACGRVSPAMLLLTEDLFAFHNHSAGGFEQDTEYGNTAHLGDCHEILPTSTTPDYKIFGGPTSDMEVSSSSVTTMASIPEITPKVIDTWRPKLTSSRKFVVQDDPNTSDFTDPLSGVRWEVQSGEANQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNGDTKSMVMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHGHGASRKETPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNIDHPLTKSSSKRDHRRSVDSRNTLNDLLKHLNDPNSNPKAIMGDIQMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAMPKNLSSPNGVLLSRQPSMNRGGYVPTPAGPKVDYIQGAPVSAHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_010809340.2
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 6D (SEMA6D), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809340.2

ORF Insert Sequence:

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC 
CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC 
TGCCACGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA 
TCTGACATGG AGGTATCTTC ATCTTCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA 
CCTAAAGTGA TTGATACCTG GAGACCTAAA CTGACCAGCT CCCGGAAATT TGTAGTTCAA 
GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCAG GTGTACGGTG GGAAGTCCAG 
TCTGGAGAGG CCAACCAGAT GGTCCACATG AATGTCCTCA TCACCTGTGT CTTTGCAGCT 
TTTGTCTTGG GTGCGTTCAT TGCAGGTGTG GCAGTCTACT GTTACCGTGA CATGTTTGTT 
CGGAAAAACA GAAAGATCCA TAAAGATGCA GAATCTGCCC AGTCATGCAC AGACTCCAGT 
GGAAGTTTTG CCAAGCTGAA TGGTCTCTTT GACAGCCCCG TCAAGGAATA CCAACAGAAT 
ATTGATTCTC CCAAATTATA TAGTAACCTG CTGACCAGTC GGAAAGAGCT GCCACCCAAT 
GGAGATACAA AATCCATGGT CATGGACCAT CGAGGCCAAC CTCCTGAGTT GGCTGCTCTC 
CCCACTCCTG AGTCTACGCC TGTGCTTCAC CAGAAGACCC TGCAGGCCAT GAAGAGCCAC 
TCAGAAAAGG CCCATGGCCA TGGAGCTTCA AGGAAAGAAA CCCCCCAGTT TTTTCCTTCT 
AGTCCTCCAC CACATTCCCC ACTAAGTCAC GGGCATATCC CCAGCGCCAT TGTTCTTCCT 
AATGCTACCC ATGACTACAA CACTTCTTTC TCAAACTCCA ATGCTCACAA AGCTGAAAAG 
AAGCTTCAAA ACATTGACCA TCCTCTTACA AAGTCATCCA GTAAAAGAGA TCACCGGCGT 
TCTGTGGATT CCAGAAATAC CCTCAATGAT CTCCTGAAGC ATCTAAATGA CCCAAACAGT 
AACCCCAAAG CCATCATGGG AGACATCCAG ATGGCCCACC AGACCCTAAT GCTGGATCCC 
GTGGGACCTA TGTCTGAAGT CCCGCCCAAG GTCCCTAACC GCGAGGCATC TCTCTACTCT 
CCTCCCTCGA CTCTTCCCAG AAATAGCCCA ACCAAGCGAG TGGATGTTCC CACCACTCCT 
GGAGTTCCGA TGACTTCTTT GGAAAGACAG AGGGGTTACC ATAAAAATTC CTCCCAGAGG 
CACTCTATAT CGGCTATGCC TAAAAACTTA AGTTCACCAA ATGGTGTTTT GTTATCTAGA 
CAGCCTAGTA TGAACCGTGG AGGGTACGTG CCCACCCCTG CAGGGCCAAA GGTGGACTAT 
ATTCAGGGAG CACCAGTGAG TGCTCACCTA CAGCCTTCCC TCTCCAGACA GAGCAGCTAC 
ACCAGTAATG GCACCCTTCC CCGGACGGGA CTAAAGAGGA CACCGTCCTT AAAACCTGAC 
GTGCCGCCAA AGCCTTCATT TGTTCCTCAA ACCACCTCCG TCAGACCACT GAACAAATAC 
ACTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA6D ( XM_010809341.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807643.1 Bos taurus semaphorin 6D (SEMA6D), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa49890
Clone ID Related Accession (Same CDS sequence)	XM_010809341.2 , XM_010809341.3
Accession Version	XM_010809341.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	semaphorin-6D isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809341.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC 
CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC 
TGCCACGACA TGGAGGTATC TTCATCTTCT GTTACCACAA TGGCAAGTAT CCCAGAAATT 
ACACCTAAAG TGATTGATAC CTGGAGACCT AAACTGACCA GCTCCCGGAA ATTTGTAGTT 
CAAGATGACC CAAACACTTC TGATTTTACT GATCCTTTAT CAGGTATCCC AAAGGGTGTA 
CGGTGGGAAG TCCAGTCTGG AGAGGCCAAC CAGATGGTCC ACATGAATGT CCTCATCACC 
TGTGTCTTTG CAGCTTTTGT CTTGGGTGCG TTCATTGCAG GTGTGGCAGT CTACTGTTAC 
CGTGACATGT TTGTTCGGAA AAACAGAAAG ATCCATAAAG ATGCAGAATC TGCCCAGTCA 
TGCACAGACT CCAGTGGAAG TTTTGCCAAG CTGAATGGTC TCTTTGACAG CCCCGTCAAG 
GAATACCAAC AGAATATTGA TTCTCCCAAA TTATATAGTA ACCTGCTGAC CAGTCGGAAA 
GAGCTGCCAC CCAATGGAGA TACAAAATCC ATGGTCATGG ACCATCGAGG CCAACCTCCT 
GAGTTGGCTG CTCTCCCCAC TCCTGAGTCT ACGCCTGTGC TTCACCAGAA GACCCTGCAG 
GCCATGAAGA GCCACTCAGA AAAGGCCCAT GGCCATGGAG CTTCAAGGAA AGAAACCCCC 
CAGTTTTTTC CTTCTAGTCC TCCACCACAT TCCCCACTAA GTCACGGGCA TATCCCCAGC 
GCCATTGTTC TTCCTAATGC TACCCATGAC TACAACACTT CTTTCTCAAA CTCCAATGCT 
CACAAAGCTG AAAAGAAGCT TCAAAACATT GACCATCCTC TTACAAAGTC ATCCAGTAAA 
AGAGATCACC GGCGTTCTGT GGATTCCAGA AATACCCTCA ATGATCTCCT GAAGCATCTA 
AATGACCCAA ACAGTAACCC CAAAGCCATC ATGGGAGACA TCCAGATGGC CCACCAGACC 
CTAATGCTGG ATCCCGTGGG ACCTATGTCT GAAGTCCCGC CCAAGGTCCC TAACCGCGAG 
GCATCTCTCT ACTCTCCTCC CTCGACTCTT CCCAGAAATA GCCCAACCAA GCGAGTGGAT 
GTTCCCACCA CTCCTGGAGT TCCGATGACT TCTTTGGAAA GACAGAGGGG TTACCATAAA 
AATTCCTCCC AGAGGCACTC TATATCGGCT ATGCCTAAAA ACTTAAGTTC ACCAAATGGT 
GTTTTGTTAT CTAGACAGCC TAGTATGAAC CGTGGAGGGT ACGTGCCCAC CCCTGCAGGG 
CCAAAGGTGG ACTATATTCA GGGAGCACCA GTGAGTGCTC ACCTACAGCC TTCCCTCTCC 
AGACAGAGCA GCTACACCAG TAATGGCACC CTTCCCCGGA CGGGACTAAA GAGGACACCG 
TCCTTAAAAC CTGACGTGCC GCCAAAGCCT TCATTTGTTC CTCAAACCAC CTCCGTCAGA 
CCACTGAACA AATACACTTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807643.1
CDS	345..3548
Translation	MRFFLLCAYMLLLLISQLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPKTEVIPNKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYKLNTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDETLSFIKSHPLMDSAVPPIADEPWFTKTRIRYRLTAIAVDHSAGPHQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNHAKCSAENEEDRKVISLQLDKDHHALYVAFSSCVIRIPLSRCERYGSCKKSCIASRDPYCGWLSQGACGRVSPAMLLLTEDLFAFHNHSAGGFEQDTEYGNTAHLGDCHDMEVSSSSVTTMASIPEITPKVIDTWRPKLTSSRKFVVQDDPNTSDFTDPLSGIPKGVRWEVQSGEANQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNGDTKSMVMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHGHGASRKETPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNIDHPLTKSSSKRDHRRSVDSRNTLNDLLKHLNDPNSNPKAIMGDIQMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAMPKNLSSPNGVLLSRQPSMNRGGYVPTPAGPKVDYIQGAPVSAHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_010809341.2
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 6D (SEMA6D), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809341.2

ORF Insert Sequence:

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC 
CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC 
TGCCACGACA TGGAGGTATC TTCATCTTCT GTTACCACAA TGGCAAGTAT CCCAGAAATT 
ACACCTAAAG TGATTGATAC CTGGAGACCT AAACTGACCA GCTCCCGGAA ATTTGTAGTT 
CAAGATGACC CAAACACTTC TGATTTTACT GATCCTTTAT CAGGTATCCC AAAGGGTGTA 
CGGTGGGAAG TCCAGTCTGG AGAGGCCAAC CAGATGGTCC ACATGAATGT CCTCATCACC 
TGTGTCTTTG CAGCTTTTGT CTTGGGTGCG TTCATTGCAG GTGTGGCAGT CTACTGTTAC 
CGTGACATGT TTGTTCGGAA AAACAGAAAG ATCCATAAAG ATGCAGAATC TGCCCAGTCA 
TGCACAGACT CCAGTGGAAG TTTTGCCAAG CTGAATGGTC TCTTTGACAG CCCCGTCAAG 
GAATACCAAC AGAATATTGA TTCTCCCAAA TTATATAGTA ACCTGCTGAC CAGTCGGAAA 
GAGCTGCCAC CCAATGGAGA TACAAAATCC ATGGTCATGG ACCATCGAGG CCAACCTCCT 
GAGTTGGCTG CTCTCCCCAC TCCTGAGTCT ACGCCTGTGC TTCACCAGAA GACCCTGCAG 
GCCATGAAGA GCCACTCAGA AAAGGCCCAT GGCCATGGAG CTTCAAGGAA AGAAACCCCC 
CAGTTTTTTC CTTCTAGTCC TCCACCACAT TCCCCACTAA GTCACGGGCA TATCCCCAGC 
GCCATTGTTC TTCCTAATGC TACCCATGAC TACAACACTT CTTTCTCAAA CTCCAATGCT 
CACAAAGCTG AAAAGAAGCT TCAAAACATT GACCATCCTC TTACAAAGTC ATCCAGTAAA 
AGAGATCACC GGCGTTCTGT GGATTCCAGA AATACCCTCA ATGATCTCCT GAAGCATCTA 
AATGACCCAA ACAGTAACCC CAAAGCCATC ATGGGAGACA TCCAGATGGC CCACCAGACC 
CTAATGCTGG ATCCCGTGGG ACCTATGTCT GAAGTCCCGC CCAAGGTCCC TAACCGCGAG 
GCATCTCTCT ACTCTCCTCC CTCGACTCTT CCCAGAAATA GCCCAACCAA GCGAGTGGAT 
GTTCCCACCA CTCCTGGAGT TCCGATGACT TCTTTGGAAA GACAGAGGGG TTACCATAAA 
AATTCCTCCC AGAGGCACTC TATATCGGCT ATGCCTAAAA ACTTAAGTTC ACCAAATGGT 
GTTTTGTTAT CTAGACAGCC TAGTATGAAC CGTGGAGGGT ACGTGCCCAC CCCTGCAGGG 
CCAAAGGTGG ACTATATTCA GGGAGCACCA GTGAGTGCTC ACCTACAGCC TTCCCTCTCC 
AGACAGAGCA GCTACACCAG TAATGGCACC CTTCCCCGGA CGGGACTAAA GAGGACACCG 
TCCTTAAAAC CTGACGTGCC GCCAAAGCCT TCATTTGTTC CTCAAACCAC CTCCGTCAGA 
CCACTGAACA AATACACTTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA6D ( XM_010809341.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807643.1 Bos taurus semaphorin 6D (SEMA6D), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa49890
Clone ID Related Accession (Same CDS sequence)	XM_010809341.2 , XM_010809341.3
Accession Version	XM_010809341.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	semaphorin-6D isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809341.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC 
CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC 
TGCCACGACA TGGAGGTATC TTCATCTTCT GTTACCACAA TGGCAAGTAT CCCAGAAATT 
ACACCTAAAG TGATTGATAC CTGGAGACCT AAACTGACCA GCTCCCGGAA ATTTGTAGTT 
CAAGATGACC CAAACACTTC TGATTTTACT GATCCTTTAT CAGGTATCCC AAAGGGTGTA 
CGGTGGGAAG TCCAGTCTGG AGAGGCCAAC CAGATGGTCC ACATGAATGT CCTCATCACC 
TGTGTCTTTG CAGCTTTTGT CTTGGGTGCG TTCATTGCAG GTGTGGCAGT CTACTGTTAC 
CGTGACATGT TTGTTCGGAA AAACAGAAAG ATCCATAAAG ATGCAGAATC TGCCCAGTCA 
TGCACAGACT CCAGTGGAAG TTTTGCCAAG CTGAATGGTC TCTTTGACAG CCCCGTCAAG 
GAATACCAAC AGAATATTGA TTCTCCCAAA TTATATAGTA ACCTGCTGAC CAGTCGGAAA 
GAGCTGCCAC CCAATGGAGA TACAAAATCC ATGGTCATGG ACCATCGAGG CCAACCTCCT 
GAGTTGGCTG CTCTCCCCAC TCCTGAGTCT ACGCCTGTGC TTCACCAGAA GACCCTGCAG 
GCCATGAAGA GCCACTCAGA AAAGGCCCAT GGCCATGGAG CTTCAAGGAA AGAAACCCCC 
CAGTTTTTTC CTTCTAGTCC TCCACCACAT TCCCCACTAA GTCACGGGCA TATCCCCAGC 
GCCATTGTTC TTCCTAATGC TACCCATGAC TACAACACTT CTTTCTCAAA CTCCAATGCT 
CACAAAGCTG AAAAGAAGCT TCAAAACATT GACCATCCTC TTACAAAGTC ATCCAGTAAA 
AGAGATCACC GGCGTTCTGT GGATTCCAGA AATACCCTCA ATGATCTCCT GAAGCATCTA 
AATGACCCAA ACAGTAACCC CAAAGCCATC ATGGGAGACA TCCAGATGGC CCACCAGACC 
CTAATGCTGG ATCCCGTGGG ACCTATGTCT GAAGTCCCGC CCAAGGTCCC TAACCGCGAG 
GCATCTCTCT ACTCTCCTCC CTCGACTCTT CCCAGAAATA GCCCAACCAA GCGAGTGGAT 
GTTCCCACCA CTCCTGGAGT TCCGATGACT TCTTTGGAAA GACAGAGGGG TTACCATAAA 
AATTCCTCCC AGAGGCACTC TATATCGGCT ATGCCTAAAA ACTTAAGTTC ACCAAATGGT 
GTTTTGTTAT CTAGACAGCC TAGTATGAAC CGTGGAGGGT ACGTGCCCAC CCCTGCAGGG 
CCAAAGGTGG ACTATATTCA GGGAGCACCA GTGAGTGCTC ACCTACAGCC TTCCCTCTCC 
AGACAGAGCA GCTACACCAG TAATGGCACC CTTCCCCGGA CGGGACTAAA GAGGACACCG 
TCCTTAAAAC CTGACGTGCC GCCAAAGCCT TCATTTGTTC CTCAAACCAC CTCCGTCAGA 
CCACTGAACA AATACACTTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807643.1
CDS	180..3383
Translation	MRFFLLCAYMLLLLISQLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPKTEVIPNKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYKLNTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDETLSFIKSHPLMDSAVPPIADEPWFTKTRIRYRLTAIAVDHSAGPHQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNHAKCSAENEEDRKVISLQLDKDHHALYVAFSSCVIRIPLSRCERYGSCKKSCIASRDPYCGWLSQGACGRVSPAMLLLTEDLFAFHNHSAGGFEQDTEYGNTAHLGDCHDMEVSSSSVTTMASIPEITPKVIDTWRPKLTSSRKFVVQDDPNTSDFTDPLSGIPKGVRWEVQSGEANQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNGDTKSMVMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHGHGASRKETPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNIDHPLTKSSSKRDHRRSVDSRNTLNDLLKHLNDPNSNPKAIMGDIQMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAMPKNLSSPNGVLLSRQPSMNRGGYVPTPAGPKVDYIQGAPVSAHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_010809341.3
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 6D (SEMA6D), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809341.3

ORF Insert Sequence:

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTGCTG TTAACTGAAG ACTTGTTTGC TTTCCATAAC 
CACAGCGCTG GAGGATTTGA ACAAGACACA GAATATGGCA ACACGGCCCA TCTAGGGGAC 
TGCCACGACA TGGAGGTATC TTCATCTTCT GTTACCACAA TGGCAAGTAT CCCAGAAATT 
ACACCTAAAG TGATTGATAC CTGGAGACCT AAACTGACCA GCTCCCGGAA ATTTGTAGTT 
CAAGATGACC CAAACACTTC TGATTTTACT GATCCTTTAT CAGGTATCCC AAAGGGTGTA 
CGGTGGGAAG TCCAGTCTGG AGAGGCCAAC CAGATGGTCC ACATGAATGT CCTCATCACC 
TGTGTCTTTG CAGCTTTTGT CTTGGGTGCG TTCATTGCAG GTGTGGCAGT CTACTGTTAC 
CGTGACATGT TTGTTCGGAA AAACAGAAAG ATCCATAAAG ATGCAGAATC TGCCCAGTCA 
TGCACAGACT CCAGTGGAAG TTTTGCCAAG CTGAATGGTC TCTTTGACAG CCCCGTCAAG 
GAATACCAAC AGAATATTGA TTCTCCCAAA TTATATAGTA ACCTGCTGAC CAGTCGGAAA 
GAGCTGCCAC CCAATGGAGA TACAAAATCC ATGGTCATGG ACCATCGAGG CCAACCTCCT 
GAGTTGGCTG CTCTCCCCAC TCCTGAGTCT ACGCCTGTGC TTCACCAGAA GACCCTGCAG 
GCCATGAAGA GCCACTCAGA AAAGGCCCAT GGCCATGGAG CTTCAAGGAA AGAAACCCCC 
CAGTTTTTTC CTTCTAGTCC TCCACCACAT TCCCCACTAA GTCACGGGCA TATCCCCAGC 
GCCATTGTTC TTCCTAATGC TACCCATGAC TACAACACTT CTTTCTCAAA CTCCAATGCT 
CACAAAGCTG AAAAGAAGCT TCAAAACATT GACCATCCTC TTACAAAGTC ATCCAGTAAA 
AGAGATCACC GGCGTTCTGT GGATTCCAGA AATACCCTCA ATGATCTCCT GAAGCATCTA 
AATGACCCAA ACAGTAACCC CAAAGCCATC ATGGGAGACA TCCAGATGGC CCACCAGACC 
CTAATGCTGG ATCCCGTGGG ACCTATGTCT GAAGTCCCGC CCAAGGTCCC TAACCGCGAG 
GCATCTCTCT ACTCTCCTCC CTCGACTCTT CCCAGAAATA GCCCAACCAA GCGAGTGGAT 
GTTCCCACCA CTCCTGGAGT TCCGATGACT TCTTTGGAAA GACAGAGGGG TTACCATAAA 
AATTCCTCCC AGAGGCACTC TATATCGGCT ATGCCTAAAA ACTTAAGTTC ACCAAATGGT 
GTTTTGTTAT CTAGACAGCC TAGTATGAAC CGTGGAGGGT ACGTGCCCAC CCCTGCAGGG 
CCAAAGGTGG ACTATATTCA GGGAGCACCA GTGAGTGCTC ACCTACAGCC TTCCCTCTCC 
AGACAGAGCA GCTACACCAG TAATGGCACC CTTCCCCGGA CGGGACTAAA GAGGACACCG 
TCCTTAAAAC CTGACGTGCC GCCAAAGCCT TCATTTGTTC CTCAAACCAC CTCCGTCAGA 
CCACTGAACA AATACACTTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA6D ( XM_010809342.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807644.1 Bos taurus semaphorin 6D (SEMA6D), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa49891
Clone ID Related Accession (Same CDS sequence)	XM_010809342.3 , XM_010809342.2
Accession Version	XM_010809342.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	semaphorin-6D isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809342.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC 
AACACGGCCC ATCTAGGGGA CTGCCACGAC ATGGAGGTAT CTTCATCTTC TGTTACCACA 
ATGGCAAGTA TCCCAGAAAT TACACCTAAA GTGATTGATA CCTGGAGACC TAAACTGACC 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAACACTT CTGATTTTAC TGATCCTTTA 
TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAGGCCAA CCAGATGGTC 
CACATGAATG TCCTCATCAC CTGTGTCTTT GCAGCTTTTG TCTTGGGTGC GTTCATTGCA 
GGTGTGGCAG TCTACTGTTA CCGTGACATG TTTGTTCGGA AAAACAGAAA GATCCATAAA 
GATGCAGAAT CTGCCCAGTC ATGCACAGAC TCCAGTGGAA GTTTTGCCAA GCTGAATGGT 
CTCTTTGACA GCCCCGTCAA GGAATACCAA CAGAATATTG ATTCTCCCAA ATTATATAGT 
AACCTGCTGA CCAGTCGGAA AGAGCTGCCA CCCAATGGAG ATACAAAATC CATGGTCATG 
GACCATCGAG GCCAACCTCC TGAGTTGGCT GCTCTCCCCA CTCCTGAGTC TACGCCTGTG 
CTTCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCAG AAAAGGCCCA TGGCCATGGA 
GCTTCAAGGA AAGAAACCCC CCAGTTTTTT CCTTCTAGTC CTCCACCACA TTCCCCACTA 
AGTCACGGGC ATATCCCCAG CGCCATTGTT CTTCCTAATG CTACCCATGA CTACAACACT 
TCTTTCTCAA ACTCCAATGC TCACAAAGCT GAAAAGAAGC TTCAAAACAT TGACCATCCT 
CTTACAAAGT CATCCAGTAA AAGAGATCAC CGGCGTTCTG TGGATTCCAG AAATACCCTC 
AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CATGGGAGAC 
ATCCAGATGG CCCACCAGAC CCTAATGCTG GATCCCGTGG GACCTATGTC TGAAGTCCCG 
CCCAAGGTCC CTAACCGCGA GGCATCTCTC TACTCTCCTC CCTCGACTCT TCCCAGAAAT 
AGCCCAACCA AGCGAGTGGA TGTTCCCACC ACTCCTGGAG TTCCGATGAC TTCTTTGGAA 
AGACAGAGGG GTTACCATAA AAATTCCTCC CAGAGGCACT CTATATCGGC TATGCCTAAA 
AACTTAAGTT CACCAAATGG TGTTTTGTTA TCTAGACAGC CTAGTATGAA CCGTGGAGGG 
TACGTGCCCA CCCCTGCAGG GCCAAAGGTG GACTATATTC AGGGAGCACC AGTGAGTGCT 
CACCTACAGC CTTCCCTCTC CAGACAGAGC AGCTACACCA GTAATGGCAC CCTTCCCCGG 
ACGGGACTAA AGAGGACACC GTCCTTAAAA CCTGACGTGC CGCCAAAGCC TTCATTTGTT 
CCTCAAACCA CCTCCGTCAG ACCACTGAAC AAATACACTT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807644.1
CDS	180..3344
Translation	MRFFLLCAYMLLLLISQLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPKTEVIPNKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYKLNTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDETLSFIKSHPLMDSAVPPIADEPWFTKTRIRYRLTAIAVDHSAGPHQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNHAKCSAENEEDRKVISLQLDKDHHALYVAFSSCVIRIPLSRCERYGSCKKSCIASRDPYCGWLSQGACGRVSPAMLAGGFEQDTEYGNTAHLGDCHDMEVSSSSVTTMASIPEITPKVIDTWRPKLTSSRKFVVQDDPNTSDFTDPLSGIPKGVRWEVQSGEANQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNGDTKSMVMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHGHGASRKETPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNIDHPLTKSSSKRDHRRSVDSRNTLNDLLKHLNDPNSNPKAIMGDIQMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAMPKNLSSPNGVLLSRQPSMNRGGYVPTPAGPKVDYIQGAPVSAHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_010809342.3
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 6D (SEMA6D), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809342.3

ORF Insert Sequence:

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC 
AACACGGCCC ATCTAGGGGA CTGCCACGAC ATGGAGGTAT CTTCATCTTC TGTTACCACA 
ATGGCAAGTA TCCCAGAAAT TACACCTAAA GTGATTGATA CCTGGAGACC TAAACTGACC 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAACACTT CTGATTTTAC TGATCCTTTA 
TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAGGCCAA CCAGATGGTC 
CACATGAATG TCCTCATCAC CTGTGTCTTT GCAGCTTTTG TCTTGGGTGC GTTCATTGCA 
GGTGTGGCAG TCTACTGTTA CCGTGACATG TTTGTTCGGA AAAACAGAAA GATCCATAAA 
GATGCAGAAT CTGCCCAGTC ATGCACAGAC TCCAGTGGAA GTTTTGCCAA GCTGAATGGT 
CTCTTTGACA GCCCCGTCAA GGAATACCAA CAGAATATTG ATTCTCCCAA ATTATATAGT 
AACCTGCTGA CCAGTCGGAA AGAGCTGCCA CCCAATGGAG ATACAAAATC CATGGTCATG 
GACCATCGAG GCCAACCTCC TGAGTTGGCT GCTCTCCCCA CTCCTGAGTC TACGCCTGTG 
CTTCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCAG AAAAGGCCCA TGGCCATGGA 
GCTTCAAGGA AAGAAACCCC CCAGTTTTTT CCTTCTAGTC CTCCACCACA TTCCCCACTA 
AGTCACGGGC ATATCCCCAG CGCCATTGTT CTTCCTAATG CTACCCATGA CTACAACACT 
TCTTTCTCAA ACTCCAATGC TCACAAAGCT GAAAAGAAGC TTCAAAACAT TGACCATCCT 
CTTACAAAGT CATCCAGTAA AAGAGATCAC CGGCGTTCTG TGGATTCCAG AAATACCCTC 
AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CATGGGAGAC 
ATCCAGATGG CCCACCAGAC CCTAATGCTG GATCCCGTGG GACCTATGTC TGAAGTCCCG 
CCCAAGGTCC CTAACCGCGA GGCATCTCTC TACTCTCCTC CCTCGACTCT TCCCAGAAAT 
AGCCCAACCA AGCGAGTGGA TGTTCCCACC ACTCCTGGAG TTCCGATGAC TTCTTTGGAA 
AGACAGAGGG GTTACCATAA AAATTCCTCC CAGAGGCACT CTATATCGGC TATGCCTAAA 
AACTTAAGTT CACCAAATGG TGTTTTGTTA TCTAGACAGC CTAGTATGAA CCGTGGAGGG 
TACGTGCCCA CCCCTGCAGG GCCAAAGGTG GACTATATTC AGGGAGCACC AGTGAGTGCT 
CACCTACAGC CTTCCCTCTC CAGACAGAGC AGCTACACCA GTAATGGCAC CCTTCCCCGG 
ACGGGACTAA AGAGGACACC GTCCTTAAAA CCTGACGTGC CGCCAAAGCC TTCATTTGTT 
CCTCAAACCA CCTCCGTCAG ACCACTGAAC AAATACACTT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA6D ( XM_010809342.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807644.1 Bos taurus semaphorin 6D (SEMA6D), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa49891
Clone ID Related Accession (Same CDS sequence)	XM_010809342.3 , XM_010809342.2
Accession Version	XM_010809342.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	semaphorin-6D isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809342.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC 
AACACGGCCC ATCTAGGGGA CTGCCACGAC ATGGAGGTAT CTTCATCTTC TGTTACCACA 
ATGGCAAGTA TCCCAGAAAT TACACCTAAA GTGATTGATA CCTGGAGACC TAAACTGACC 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAACACTT CTGATTTTAC TGATCCTTTA 
TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAGGCCAA CCAGATGGTC 
CACATGAATG TCCTCATCAC CTGTGTCTTT GCAGCTTTTG TCTTGGGTGC GTTCATTGCA 
GGTGTGGCAG TCTACTGTTA CCGTGACATG TTTGTTCGGA AAAACAGAAA GATCCATAAA 
GATGCAGAAT CTGCCCAGTC ATGCACAGAC TCCAGTGGAA GTTTTGCCAA GCTGAATGGT 
CTCTTTGACA GCCCCGTCAA GGAATACCAA CAGAATATTG ATTCTCCCAA ATTATATAGT 
AACCTGCTGA CCAGTCGGAA AGAGCTGCCA CCCAATGGAG ATACAAAATC CATGGTCATG 
GACCATCGAG GCCAACCTCC TGAGTTGGCT GCTCTCCCCA CTCCTGAGTC TACGCCTGTG 
CTTCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCAG AAAAGGCCCA TGGCCATGGA 
GCTTCAAGGA AAGAAACCCC CCAGTTTTTT CCTTCTAGTC CTCCACCACA TTCCCCACTA 
AGTCACGGGC ATATCCCCAG CGCCATTGTT CTTCCTAATG CTACCCATGA CTACAACACT 
TCTTTCTCAA ACTCCAATGC TCACAAAGCT GAAAAGAAGC TTCAAAACAT TGACCATCCT 
CTTACAAAGT CATCCAGTAA AAGAGATCAC CGGCGTTCTG TGGATTCCAG AAATACCCTC 
AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CATGGGAGAC 
ATCCAGATGG CCCACCAGAC CCTAATGCTG GATCCCGTGG GACCTATGTC TGAAGTCCCG 
CCCAAGGTCC CTAACCGCGA GGCATCTCTC TACTCTCCTC CCTCGACTCT TCCCAGAAAT 
AGCCCAACCA AGCGAGTGGA TGTTCCCACC ACTCCTGGAG TTCCGATGAC TTCTTTGGAA 
AGACAGAGGG GTTACCATAA AAATTCCTCC CAGAGGCACT CTATATCGGC TATGCCTAAA 
AACTTAAGTT CACCAAATGG TGTTTTGTTA TCTAGACAGC CTAGTATGAA CCGTGGAGGG 
TACGTGCCCA CCCCTGCAGG GCCAAAGGTG GACTATATTC AGGGAGCACC AGTGAGTGCT 
CACCTACAGC CTTCCCTCTC CAGACAGAGC AGCTACACCA GTAATGGCAC CCTTCCCCGG 
ACGGGACTAA AGAGGACACC GTCCTTAAAA CCTGACGTGC CGCCAAAGCC TTCATTTGTT 
CCTCAAACCA CCTCCGTCAG ACCACTGAAC AAATACACTT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807644.1
CDS	345..3509
Translation	MRFFLLCAYMLLLLISQLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLYIAGRDQVYTVNLNEIPKTEVIPNKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYKLNTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRTIKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKARLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDETLSFIKSHPLMDSAVPPIADEPWFTKTRIRYRLTAIAVDHSAGPHQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNHAKCSAENEEDRKVISLQLDKDHHALYVAFSSCVIRIPLSRCERYGSCKKSCIASRDPYCGWLSQGACGRVSPAMLAGGFEQDTEYGNTAHLGDCHDMEVSSSSVTTMASIPEITPKVIDTWRPKLTSSRKFVVQDDPNTSDFTDPLSGIPKGVRWEVQSGEANQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNGDTKSMVMDHRGQPPELAALPTPESTPVLHQKTLQAMKSHSEKAHGHGASRKETPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSNAHKAEKKLQNIDHPLTKSSSKRDHRRSVDSRNTLNDLLKHLNDPNSNPKAIMGDIQMAHQTLMLDPVGPMSEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAMPKNLSSPNGVLLSRQPSMNRGGYVPTPAGPKVDYIQGAPVSAHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPKPSFVPQTTSVRPLNKYTY

Target ORF information:

RefSeq Version	XM_010809342.2
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 6D (SEMA6D), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809342.2

ORF Insert Sequence:

ATGAGGTTCT TCCTGCTCTG TGCCTACATG CTGCTGCTAC TGATTTCCCA GTTGAGGGCA 
GTCAGCTTTC CTGAAGATGA TGAACCCCTT AATACTGTTG ACTATCACTA TTCAAGGCAA 
TATCCGGTTT TTAGAGGACG TCCTTCAGGC AATGAATCAC AGCACAGGCT GGACTTTCAG 
CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA 
AACTTAAATG AAATCCCCAA AACAGAAGTA ATACCAAACA AGAAACTGAC ATGGCGGTCA 
AGACAACAGG ATCGAGAAAA CTGTGCTATG AAAGGCAAGC ATAAAGATGA ATGCCACAAC 
TTTATTAAAG TATTTGTTCC AAGAAACGAT GAGATGGTTT TTGTTTGTGG CACCAATGCG 
TTTAATCCCA TGTGTAGATA CTATAAGTTG AATACCTTAG AGTATGATGG AGAAGAAATT 
AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACTA ATGTTGCCCT TTTTGCTGAT 
GGGAAGCTGT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA 
AGCATGGGTG ATGGATCTGC TCTTCGTACA ATAAAATATG ATTCCAAATG GATCAAAGAG 
CCACACTTTC TGCATGCTAT AGAATACGGA AACTACGTCT ATTTCTTCTT TCGAGAAATT 
GCTGTAGAAC ATAACAACTT AGGCAAGGCT GTCTATTCAC GTGTGGCCCG CATATGTAAA 
AACGACATGG GTGGCTCCCA GCGCGTCCTG GAGAAGCACT GGACTTCGTT TCTGAAGGCT 
CGGCTTAACT GTTCTGTCCC TGGAGATTCC TTTTTCTACT TTGATGTTCT GCAGTCTATT 
ACAGACATAA TACAAATCAA TGGCATTCCC ACAGTGGTAG GGGTTTTCAC CACACAGCTC 
AACAGCATTC CTGGTTCCGC AGTCTGTGCG TTTAGCATGG ATGACATTGA AAAAGTATTC 
AAAGGACGGT TTAAAGAACA GAAAACTCCA GATTCTGTTT GGACAGCGGT CCCTGAAGAC 
AAAGTACCAA AGCCAAGGCC TGGGTGTTGT GCAAAGCACG GTCTTGCGGA AGCATATAAA 
ACCTCCATCG ATTTCCCGGA TGAAACCCTG TCATTCATCA AATCCCACCC CCTGATGGAC 
TCGGCCGTCC CACCCATTGC CGACGAACCC TGGTTCACAA AGACTCGGAT CAGGTACAGA 
CTGACGGCCA TCGCCGTCGA CCATTCTGCC GGACCCCACC AGAACTACAC AGTCATCTTT 
GTTGGCTCAG AAGCTGGCGT GGTGCTTAAA GTTTTGGCGA AGACCAGCCC TTTCTCTTTG 
AATGACAGCG TATTACTGGA AGAGATTGAA GCATACAACC ATGCAAAGTG CAGTGCTGAA 
AATGAGGAGG ACAGAAAGGT CATCTCATTA CAGTTGGATA AAGACCATCA TGCTTTATAT 
GTGGCGTTCT CTAGCTGCGT TATCCGCATC CCCCTCAGTC GCTGTGAGCG TTATGGATCA 
TGTAAAAAGT CTTGTATTGC ATCTCGAGAC CCATACTGTG GCTGGTTAAG CCAAGGGGCC 
TGTGGTCGAG TGAGCCCAGC GATGCTCGCT GGAGGATTTG AACAAGACAC AGAATATGGC 
AACACGGCCC ATCTAGGGGA CTGCCACGAC ATGGAGGTAT CTTCATCTTC TGTTACCACA 
ATGGCAAGTA TCCCAGAAAT TACACCTAAA GTGATTGATA CCTGGAGACC TAAACTGACC 
AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAACACTT CTGATTTTAC TGATCCTTTA 
TCAGGTATCC CAAAGGGTGT ACGGTGGGAA GTCCAGTCTG GAGAGGCCAA CCAGATGGTC 
CACATGAATG TCCTCATCAC CTGTGTCTTT GCAGCTTTTG TCTTGGGTGC GTTCATTGCA 
GGTGTGGCAG TCTACTGTTA CCGTGACATG TTTGTTCGGA AAAACAGAAA GATCCATAAA 
GATGCAGAAT CTGCCCAGTC ATGCACAGAC TCCAGTGGAA GTTTTGCCAA GCTGAATGGT 
CTCTTTGACA GCCCCGTCAA GGAATACCAA CAGAATATTG ATTCTCCCAA ATTATATAGT 
AACCTGCTGA CCAGTCGGAA AGAGCTGCCA CCCAATGGAG ATACAAAATC CATGGTCATG 
GACCATCGAG GCCAACCTCC TGAGTTGGCT GCTCTCCCCA CTCCTGAGTC TACGCCTGTG 
CTTCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCAG AAAAGGCCCA TGGCCATGGA 
GCTTCAAGGA AAGAAACCCC CCAGTTTTTT CCTTCTAGTC CTCCACCACA TTCCCCACTA 
AGTCACGGGC ATATCCCCAG CGCCATTGTT CTTCCTAATG CTACCCATGA CTACAACACT 
TCTTTCTCAA ACTCCAATGC TCACAAAGCT GAAAAGAAGC TTCAAAACAT TGACCATCCT 
CTTACAAAGT CATCCAGTAA AAGAGATCAC CGGCGTTCTG TGGATTCCAG AAATACCCTC 
AATGATCTCC TGAAGCATCT AAATGACCCA AACAGTAACC CCAAAGCCAT CATGGGAGAC 
ATCCAGATGG CCCACCAGAC CCTAATGCTG GATCCCGTGG GACCTATGTC TGAAGTCCCG 
CCCAAGGTCC CTAACCGCGA GGCATCTCTC TACTCTCCTC CCTCGACTCT TCCCAGAAAT 
AGCCCAACCA AGCGAGTGGA TGTTCCCACC ACTCCTGGAG TTCCGATGAC TTCTTTGGAA 
AGACAGAGGG GTTACCATAA AAATTCCTCC CAGAGGCACT CTATATCGGC TATGCCTAAA 
AACTTAAGTT CACCAAATGG TGTTTTGTTA TCTAGACAGC CTAGTATGAA CCGTGGAGGG 
TACGTGCCCA CCCCTGCAGG GCCAAAGGTG GACTATATTC AGGGAGCACC AGTGAGTGCT 
CACCTACAGC CTTCCCTCTC CAGACAGAGC AGCTACACCA GTAATGGCAC CCTTCCCCGG 
ACGGGACTAA AGAGGACACC GTCCTTAAAA CCTGACGTGC CGCCAAAGCC TTCATTTGTT 
CCTCAAACCA CCTCCGTCAG ACCACTGAAC AAATACACTT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SEMA6D
Entrez Gene ID	518458
Full Name	semaphorin 6D
General protein information	Preferred Names semaphorin 6D Names semaphorin-6D sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D
Gene Type	protein-coding
Organism	Bos taurus(cattle)

	SEMA6D	XP_001112236.1
Canis lupus familiaris (dog)	SEMA6D	XP_005638436.1
Bos taurus (cattle)	SEMA6D	NP_001178062.1
Homo sapiens (human)	SEMA6D	NP_705871.1
Pan troglodytes (chimpanzee)	SEMA6D	XP_003314766.1
Danio rerio (zebrafish)	sema6dl	NP_001017682.2
Mus musculus (house mouse)	Sema6d	NP_954711.1
Rattus norvegicus (Norway rat)	Sema6d	NP_001101238.1
Gallus gallus (chicken)	SEMA6D	XP_001231859.2
Xenopus tropicalis (tropical clawed frog)	sema6d	NP_001120152.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SEMA6D cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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