Itgb4 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_017314350.1	XP_017169839.1	integrin beta-4 isoform X2
XM_017314349.2	XP_017169838.1	integrin beta-4 isoform X1
XM_006532574.2	XP_006532637.1	integrin beta-4 isoform X6
NM_133663.2	NP_598424.2	integrin beta-4 isoform 2 precursor
XM_017314351.1	XP_017169840.1	integrin beta-4 isoform X3
XM_017314351.2	XP_017169840.1	integrin beta-4 isoform X3
XM_006532572.4	XP_006532635.1	integrin beta-4 isoform X4
XM_006532573.4	XP_006532636.1	integrin beta-4 isoform X5
XM_017314349.1	XP_017169838.1	integrin beta-4 isoform X1
XM_006532572.3	XP_006532635.1	integrin beta-4 isoform X4
XM_006532573.3	XP_006532636.1	integrin beta-4 isoform X5
XM_017314350.2	XP_017169839.1	integrin beta-4 isoform X2
NM_001005608.2	NP_001005608.2	integrin beta-4 isoform 1 precursor

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu45808	XM_006532572.4 Latest version!	Mus musculus integrin beta 4 (Itgb4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu45809	XM_006532573.4 Latest version!	Mus musculus integrin beta 4 (Itgb4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu77781	XM_017314349.2 Latest version!	Mus musculus integrin beta 4 (Itgb4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu77782	XM_017314350.2 Latest version!	Mus musculus integrin beta 4 (Itgb4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu77783	XM_017314351.2 Latest version!	Mus musculus integrin beta 4 (Itgb4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu17496	NM_133663.2 Latest version!	Mus musculus integrin beta 4 (Itgb4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu17669	NM_001005608.2 Latest version!	Mus musculus integrin beta 4 (Itgb4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu45810	XM_006532574.2 Latest version!	Mus musculus integrin beta 4 (Itgb4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OMu77783	XM_017314351.1	Mus musculus integrin beta 4 (Itgb4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu77781	XM_017314349.1	Mus musculus integrin beta 4 (Itgb4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu45808	XM_006532572.3	Mus musculus integrin beta 4 (Itgb4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu45809	XM_006532573.3	Mus musculus integrin beta 4 (Itgb4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu77782	XM_017314350.1	Mus musculus integrin beta 4 (Itgb4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

Itgb4 ( XM_006532572.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532635.1 Mus musculus integrin beta 4 (Itgb4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu45808
Clone ID Related Accession (Same CDS sequence)	XM_006532572.4 , XM_006532572.3
Accession Version	XM_006532572.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	integrin beta-4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532572.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGC CCTGTTGCAG CCCATGGGTG AAGCTGCTGC TGCTGGCAGC AATGCTGAGT 
GCCAGCCTCC CTGGAGACCT GGCCAACCGC TGCAAGAAGG CTCAGGTGAA GAGCTGTACC 
GAGTGCATCC GGGTGGACAA GAGCTGTGCC TACTGCACAG ACGAGCTGTT CAAGGAGAGG 
CGCTGCAACA CCCAGGCGGA GCTTCTGGCT GCAGGCTGCA GGGGAGAGAG CATCCTGGTC 
ATGGAGAGCA GCCTTGAAAT CACAGAGAAC ACCCAGATCG ACACCAGCCT GCACCGCAGC 
CAGGTATCTC CCCAAGGCCT GCAAGTCCGG CTGCGGCCGG GTGAGGAGCG CAGCTTTGTG 
TTCCAGGTGT TTGAGCCCCT GGAGAGCCCC GTGGATCTGT ATATCCTCAT GGACTTCTCC 
AACTCCATGT CTGACGATCT GGACAACCTC AAGCAGATGG GGCAGAACCT GGCCAAGATC 
CTGCGCCAGC TCACCAGCGA CTACACCATT GGATTTGGAA AGTTTGTGGA CAAAGTCAGC 
GTCCCACAGA CAGACATGAG GCCCGAGAAA CTGAAGGAGC CCTGGCCCAA CAGTGATCCC 
CCGTTCTCCT TCAAGAACGT TATCAGCTTA ACGGAGAATG TGGAAGAATT CTGGAACAAA 
CTGCAAGGAG AACGCATCTC AGGCAACCTG GACGCTCCTG AAGGGGGCTT TGATGCCATC 
CTGCAGACAG CTGTGTGCAC AAGGGACATT GGCTGGAGGG CTGACAGCAC CCACCTGCTG 
GTGTTCTCCA CCGAGTCTGC CTTCCACTAC GAGGCTGATG GTGCCAACGT GCTGGCCGGC 
ATCATGAACC GCAATGATGA GAAATGCCAC CTGGACGCCT CGGGCGCCTA CACCCAATAC 
AAGACACAGG ACTACCCATC AGTGCCCACG CTGGTTCGCC TGCTTGCCAA GCATAACATC 
ATCCCCATCT TTGCTGTCAC CAACTACTCT TACAGCTACT ATGAGAAGCT CCATAAGTAT 
TTCCCCGTCT CCTCTCTGGG CGTCCTGCAG GAGGATTCAT CCAACATCGT GGAGCTGCTG 
GAGGAGGCCT TCTATCGAAT TCGCTCCAAC CTGGACATCC GGGCTCTGGA CAGCCCCAGA 
GGCCTGAGAA CAGAGGTCAC CTCCGATACT CTCCAGAAGA CGGAGACTGG GTCCTTTCAC 
ATCAAGCGGG GGGAAGTGGG CACATACAAT GTGCATCTCC GGGCAGTGGA GGACATAGAT 
GGGACACATG TGTGCCAGCT GGCTAAAGAA GACCAAGGGG GCAACATCCA CCTGAAACCC 
TCCTTCTCTG ATGGCCTCCG GATGGACGCG AGTGTGATCT GTGACGTGTG CCCCTGTGAG 
CTGCAAAAGG AAGTTCGATC AGCTCGCTGT CACTTCAGAG GAGACTTCAT GTGTGGACAC 
TGTGTGTGCA ATGAGGGCTG GAGTGGCAAA ACCTGCAACT GCTCCACCGG CTCTCTGAGT 
GACACACAGC CCTGCCTGCG TGAGGGTGAG GACAAACCGT GCTCGGGCCA CGGCGAGTGC 
CAGTGCGGAC GCTGTGTGTG CTATGGTGAA GGCCGCTACG AGGGTCACTT CTGCGAGTAT 
GACAACTTCC AGTGTCCCCG GACCTCTGGA TTCCTGTGCA ATGACCGGGG TCGCTGTTCT 
ATGGGAGAGT GTGTGTGTGA GCCTGGTTGG ACAGGCCGCA GCTGTGACTG TCCCCTCAGC 
AATGCCACCT GCATCGATAG CAACGGGGGC ATCTGCAACG GCCGAGGCTA CTGTGAGTGT 
GGCCGTTGTC ACTGCAACCA GCAGTCGCTC TACACGGACA CCACCTGTGA GATCAACTAC 
TCTGCGATAC GCCTGGGTCT CTGTGAGGAT CTCCGCTCCT GCGTACAGTG CCAGGCCTGG 
GGCACCGGGG AGAAGAAAGG GCGCGCGTGT GACGATTGCC CCTTTAAAGT CAAGATGGTA 
GACGAGCTTA AGAAAGCAGA AGAGGTGGTG GAGTACTGCT CCTTCCGGGA TGAGGATGAC 
GACTGCACTT ACAGCTACAA CGTGGAGGGC GATGGCAGCC CTGGGCCCAA CAGCACAGTC 
CTGGTCCACA AAAAGAAAGA CTGCCCTCCC GGCTCCTTCT GGTGGCTCAT CCCCCTGCTC 
ATCTTCCTCC TGTTGCTCCT GGCGTTGCTT CTGCTGCTCT GCTGGAAATA CTGTGCCTGC 
TGCAAAGCCT GCCTGGGGCT TCTTCCTTGC TGCAACCGAG GTCACATGGT GGGCTTTAAG 
GAAGATCACT ATATGCTTCG GGAGAACCTG ATGGCCTCTG ACCACCTGGA CACGCCCATG 
CTACGAAGCG GGAACCTCAA GGGACGAGAC ACAGTCCGCT GGAAGATCAC CAACAATGTG 
CAGCGCCCTG GCTTTGCCAC CCATGCCGCC AGCACCAGCC CCACGGAGCT CGTACCCTAC 
GGGCTGTCCC TGCGCCTTGG CCGCCTCTGC ACTGAGAACC TTATGAAGCC GGGCACCCGA 
GAGTGTGACC AGCTACGCCA GGAGGTGGAG GAAAATCTGA ATGAGGTGTA TAGACAGGTC 
AGCGGCGCAC ACAAGCTCCA GCAGACGAAG TTCCGACAGC AGCCCAACAC CGGGAAAAAG 
CAAGACCACA CCATTGTGGA CACAGTGTTG CTGGCGCCCC GCTCCGCCAA GCAGATGCTG 
CTGAAGCTGA CAGAGAAGCA GGTGGAGCAG GGGTCCTTCC ATGAACTGAA GGTGGCCCCT 
GGCTACTACA CTGTCACGGC AGAGCAGGAT GCCCGGGGCA TGGTGGAGTT CCAGGAGGGC 
GTGGAGCTGG TGGATGTGCG AGTGCCCCTC TTCATCCGGC CTGAGGATGA TGATGAGAAG 
CAGCTGCTGG TGGAGGCCAT TGATGTCCCT GTGGGCACTG CCACCCTTGG TCGCCGTCTG 
GTAAACATCA CCATTATCAA GGAACAAGCT AGTGGGGTAG TGTCCTTCGA GCAGCCTGAA 
TACTCGGTGA GTCGTGGAGA CCAGGTGGCC CGCATCCCTG TCATCCGGCA CATCCTGGAC 
AATGGCAAGT CCCAGGTCTC CTATAGCACA CAGGATAATA CAGCACACGG ACACCGGGAT 
TATGTCCCTG TGGAGGGAGA GCTGCTGTTC CATCCTGGGG AGACCTGGAA GGAGTTGCAG 
GTGAAGCTAC TGGAGCTGCA GGAGGTCGAC TCCCTCCTGC GTGGCCGCCA GGTCCGCCGC 
TTCCAAGTCC AACTCAGCAA CCCCAAGTTC GGAGCCCGCC TGGGCCAGCC CAGCACAACC 
ACCGTTATTC TCGGTGAGCA CGATGAAACG GACAGGAGTC TCATAAATCA AACACTTTCA 
TCGCCTCCGC CACCCCATGG AGACCTGGGC GCCCCACAGA ACCCCAATGC CAAGGCTGCC 
GGGTCCAGGA AGATCCATTT CAACTGGCTG CCCCCTCCTG GCAAGCCAAT GGGGTACAGG 
GTGAAGTACT GGATCCAGGG CGACTCTGAG TCTGAAGCCC ACCTTCTAGA CAGCAAGGTG 
CCCTCAGTGG AACTCACCAA CCTGTATCCC TATTGCGACT ACGAAATGAA GGTGTGTGCC 
TATGGGGCAC AGGGTGAGGG GCCCTATAGC TCACTGGTGT CCTGCCGCAC CCACCAGGAA 
GTACCCAGCG AGCCAGGGAG GCTGGCTTTC AATGTAGTCT CTTCTACGGT GACGCAGCTG 
AGCTGGGCAG AGCCAGCTGA GACCAATGGC GAGATCACAG CCTACGAGGT CTGCTATGGA 
CTGGTCAATG AGGACAACCG ACCCATTGGA CCTATGAAGA AGGTGCTCGT GGACAACCCC 
AAGAACCGGA TGCTGCTCAT TGAGAATCTG CGAGAATCCC AGCCATACCG ATACACGGTT 
AAGGCGCGCA ATGGGGCAGG ATGGGGACCC GAGAGAGAGG CTATCATCAA CCTGGCTACA 
CAGCCCAAGC GGCCCATGTC CATCCCTATC ATCCCAGACA TCCCCATAGT GGACGCCCAG 
GGTGGAGAAG ACTACGAAAA CTTCCTTATG TACAGTGATG ACGTCCTGCG GTCCCCAGCC 
AGCAGCCAGA GGCCCAGCGT TTCTGATGAC ACTGGCTGCG GCTGGAAGTT CGAGCCTCTG 
CTCGGGGAGG AGCTGGACCT GCGGCGCGTC ACGTGGCGGC TACCCCCGGA ACTCATCCCG 
CGCCTGTCCG CCAGCAGCGG GCGCTCTGAC GAAGACGGCA GCGTTGCGGG GGGCGTGGAA 
GGGGAGGGCA GCGGCTGGAT CCGCGGGGCT ACGCCCCGAC CCCCGGGAGA GCACCTGGTG 
AATGGCCGGA TGGACTTTGC CTATCCAGGC AGCGCCAACT CCCTGCACAG AATGACTGCA 
GCCAATGTGG CCTATGGCAC GCATCTGAGC CCACACCTGT CCCACCGAGT GCTGAGCACG 
TCCTCCACCC TTACCCGGGA CTACCACTCT CTGACACGCA CAGAGCACTC CCACTCAGGC 
ACACTTCCCA GGGACTACTC CACCCTCACT TCCCTTTCCT CCCAAGATTC CCGTGGGGCT 
GTGGGTGTGC CTGACACACC CACTCGGCTG GTGTTCTCTG CCCTGGGGCC CACGTCTTTG 
AAGGTGAGCT GGCAGGAGCC ACAGTGTGAT CGGATGCTGC TGGGCTACAG TGTGGAATAC 
CAGCTACTAA ACGGCGGCGA GATGCATCGG CTCAACATCC CTAACCCTGG CCAAACCTCG 
GTGGTGGTAG AGGATCTCCT GCCTAACCAC TCCTATGTGT TCCGGGTACG GGCCCAGAGC 
CAGGAGGGCT GGGGCCGAGA GCGAGAGGGT GTCATCACCA TCGAGTCCCA GGTGCACCCG 
CAGAGCCCTC TCTGCCCCCT GCCAGGCTCA GCCTTCACTC TGAGCACCCC CAGCGCCCCG 
GGACCGCTGG TGTTCACTGC CCTAAGCCCA GACTCCCTGC AGCTCAGCTG GGAGCGGCCG 
AGGAGGCCCA ATGGAGATAT CCTTGGCTAC CTGGTGACCT GTGAGATGGC CCAAGGAGGA 
GCACCAGCCA GGACCTTCCG GGTGGACGGA GACAACCCTG AGAGCCGGTT GACTGTACCT 
GGCCTCAGTG AGAACGTTCC TTACAAGTTC AAGGTTCAGG CCAGGACGAC CGAGGGCTTC 
GGGCCAGAGC GTGAGGGTAT CATCACCATC GAGTCTCAGG TTGGAGGCCC CTTCCCACAG 
CTGGGCAGCC ATTCTGGGCT CTTCCAGAAC CCAGTGCAAA GCGAGTTCAG CAGCGTGACC 
AGCACGCACA GCACCACGAC TGAGCCCTTC CTCATGGATG GTCTAACCCT GGGGACCCAG 
CGCCTGGAAG CAGGAGGCTC CCTCACCCGG CATGTGACCC AGGAATTCGT GACCCGGACC 
TTAACGGCCA GTGGCTCTCT CAGCACTCAT ATGGACCAAC AGTTCTTCCA AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532635.1
CDS	436..5892
Translation	MAGPCCSPWVKLLLLAAMLSASLPGDLANRCKKAQVKSCTECIRVDKSCAYCTDELFKERRCNTQAELLAAGCRGESILVMESSLEITENTQIDTSLHRSQVSPQGLQVRLRPGEERSFVFQVFEPLESPVDLYILMDFSNSMSDDLDNLKQMGQNLAKILRQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTENVEEFWNKLQGERISGNLDAPEGGFDAILQTAVCTRDIGWRADSTHLLVFSTESAFHYEADGANVLAGIMNRNDEKCHLDASGAYTQYKTQDYPSVPTLVRLLAKHNIIPIFAVTNYSYSYYEKLHKYFPVSSLGVLQEDSSNIVELLEEAFYRIRSNLDIRALDSPRGLRTEVTSDTLQKTETGSFHIKRGEVGTYNVHLRAVEDIDGTHVCQLAKEDQGGNIHLKPSFSDGLRMDASVICDVCPCELQKEVRSARCHFRGDFMCGHCVCNEGWSGKTCNCSTGSLSDTQPCLREGEDKPCSGHGECQCGRCVCYGEGRYEGHFCEYDNFQCPRTSGFLCNDRGRCSMGECVCEPGWTGRSCDCPLSNATCIDSNGGICNGRGYCECGRCHCNQQSLYTDTTCEINYSAIRLGLCEDLRSCVQCQAWGTGEKKGRACDDCPFKVKMVDELKKAEEVVEYCSFRDEDDDCTYSYNVEGDGSPGPNSTVLVHKKKDCPPGSFWWLIPLLIFLLLLLALLLLLCWKYCACCKACLGLLPCCNRGHMVGFKEDHYMLRENLMASDHLDTPMLRSGNLKGRDTVRWKITNNVQRPGFATHAASTSPTELVPYGLSLRLGRLCTENLMKPGTRECDQLRQEVEENLNEVYRQVSGAHKLQQTKFRQQPNTGKKQDHTIVDTVLLAPRSAKQMLLKLTEKQVEQGSFHELKVAPGYYTVTAEQDARGMVEFQEGVELVDVRVPLFIRPEDDDEKQLLVEAIDVPVGTATLGRRLVNITIIKEQASGVVSFEQPEYSVSRGDQVARIPVIRHILDNGKSQVSYSTQDNTAHGHRDYVPVEGELLFHPGETWKELQVKLLELQEVDSLLRGRQVRRFQVQLSNPKFGARLGQPSTTTVILGEHDETDRSLINQTLSSPPPPHGDLGAPQNPNAKAAGSRKIHFNWLPPPGKPMGYRVKYWIQGDSESEAHLLDSKVPSVELTNLYPYCDYEMKVCAYGAQGEGPYSSLVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYGLVNEDNRPIGPMKKVLVDNPKNRMLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDIPIVDAQGGEDYENFLMYSDDVLRSPASSQRPSVSDDTGCGWKFEPLLGEELDLRRVTWRLPPELIPRLSASSGRSDEDGSVAGGVEGEGSGWIRGATPRPPGEHLVNGRMDFAYPGSANSLHRMTAANVAYGTHLSPHLSHRVLSTSSTLTRDYHSLTRTEHSHSGTLPRDYSTLTSLSSQDSRGAVGVPDTPTRLVFSALGPTSLKVSWQEPQCDRMLLGYSVEYQLLNGGEMHRLNIPNPGQTSVVVEDLLPNHSYVFRVRAQSQEGWGREREGVITIESQVHPQSPLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPNGDILGYLVTCEMAQGGAPARTFRVDGDNPESRLTVPGLSENVPYKFKVQARTTEGFGPEREGIITIESQVGGPFPQLGSHSGLFQNPVQSEFSSVTSTHSTTTEPFLMDGLTLGTQRLEAGGSLTRHVTQEFVTRTLTASGSLSTHMDQQFFQT

Target ORF information:

RefSeq Version	XM_006532572.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus integrin beta 4 (Itgb4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532572.4

ORF Insert Sequence:

ATGGCAGGGC CCTGTTGCAG CCCATGGGTG AAGCTGCTGC TGCTGGCAGC AATGCTGAGT 
GCCAGCCTCC CTGGAGACCT GGCCAACCGC TGCAAGAAGG CTCAGGTGAA GAGCTGTACC 
GAGTGCATCC GGGTGGACAA GAGCTGTGCC TACTGCACAG ACGAGCTGTT CAAGGAGAGG 
CGCTGCAACA CCCAGGCGGA GCTTCTGGCT GCAGGCTGCA GGGGAGAGAG CATCCTGGTC 
ATGGAGAGCA GCCTTGAAAT CACAGAGAAC ACCCAGATCG ACACCAGCCT GCACCGCAGC 
CAGGTATCTC CCCAAGGCCT GCAAGTCCGG CTGCGGCCGG GTGAGGAGCG CAGCTTTGTG 
TTCCAGGTGT TTGAGCCCCT GGAGAGCCCC GTGGATCTGT ATATCCTCAT GGACTTCTCC 
AACTCCATGT CTGACGATCT GGACAACCTC AAGCAGATGG GGCAGAACCT GGCCAAGATC 
CTGCGCCAGC TCACCAGCGA CTACACCATT GGATTTGGAA AGTTTGTGGA CAAAGTCAGC 
GTCCCACAGA CAGACATGAG GCCCGAGAAA CTGAAGGAGC CCTGGCCCAA CAGTGATCCC 
CCGTTCTCCT TCAAGAACGT TATCAGCTTA ACGGAGAATG TGGAAGAATT CTGGAACAAA 
CTGCAAGGAG AACGCATCTC AGGCAACCTG GACGCTCCTG AAGGGGGCTT TGATGCCATC 
CTGCAGACAG CTGTGTGCAC AAGGGACATT GGCTGGAGGG CTGACAGCAC CCACCTGCTG 
GTGTTCTCCA CCGAGTCTGC CTTCCACTAC GAGGCTGATG GTGCCAACGT GCTGGCCGGC 
ATCATGAACC GCAATGATGA GAAATGCCAC CTGGACGCCT CGGGCGCCTA CACCCAATAC 
AAGACACAGG ACTACCCATC AGTGCCCACG CTGGTTCGCC TGCTTGCCAA GCATAACATC 
ATCCCCATCT TTGCTGTCAC CAACTACTCT TACAGCTACT ATGAGAAGCT CCATAAGTAT 
TTCCCCGTCT CCTCTCTGGG CGTCCTGCAG GAGGATTCAT CCAACATCGT GGAGCTGCTG 
GAGGAGGCCT TCTATCGAAT TCGCTCCAAC CTGGACATCC GGGCTCTGGA CAGCCCCAGA 
GGCCTGAGAA CAGAGGTCAC CTCCGATACT CTCCAGAAGA CGGAGACTGG GTCCTTTCAC 
ATCAAGCGGG GGGAAGTGGG CACATACAAT GTGCATCTCC GGGCAGTGGA GGACATAGAT 
GGGACACATG TGTGCCAGCT GGCTAAAGAA GACCAAGGGG GCAACATCCA CCTGAAACCC 
TCCTTCTCTG ATGGCCTCCG GATGGACGCG AGTGTGATCT GTGACGTGTG CCCCTGTGAG 
CTGCAAAAGG AAGTTCGATC AGCTCGCTGT CACTTCAGAG GAGACTTCAT GTGTGGACAC 
TGTGTGTGCA ATGAGGGCTG GAGTGGCAAA ACCTGCAACT GCTCCACCGG CTCTCTGAGT 
GACACACAGC CCTGCCTGCG TGAGGGTGAG GACAAACCGT GCTCGGGCCA CGGCGAGTGC 
CAGTGCGGAC GCTGTGTGTG CTATGGTGAA GGCCGCTACG AGGGTCACTT CTGCGAGTAT 
GACAACTTCC AGTGTCCCCG GACCTCTGGA TTCCTGTGCA ATGACCGGGG TCGCTGTTCT 
ATGGGAGAGT GTGTGTGTGA GCCTGGTTGG ACAGGCCGCA GCTGTGACTG TCCCCTCAGC 
AATGCCACCT GCATCGATAG CAACGGGGGC ATCTGCAACG GCCGAGGCTA CTGTGAGTGT 
GGCCGTTGTC ACTGCAACCA GCAGTCGCTC TACACGGACA CCACCTGTGA GATCAACTAC 
TCTGCGATAC GCCTGGGTCT CTGTGAGGAT CTCCGCTCCT GCGTACAGTG CCAGGCCTGG 
GGCACCGGGG AGAAGAAAGG GCGCGCGTGT GACGATTGCC CCTTTAAAGT CAAGATGGTA 
GACGAGCTTA AGAAAGCAGA AGAGGTGGTG GAGTACTGCT CCTTCCGGGA TGAGGATGAC 
GACTGCACTT ACAGCTACAA CGTGGAGGGC GATGGCAGCC CTGGGCCCAA CAGCACAGTC 
CTGGTCCACA AAAAGAAAGA CTGCCCTCCC GGCTCCTTCT GGTGGCTCAT CCCCCTGCTC 
ATCTTCCTCC TGTTGCTCCT GGCGTTGCTT CTGCTGCTCT GCTGGAAATA CTGTGCCTGC 
TGCAAAGCCT GCCTGGGGCT TCTTCCTTGC TGCAACCGAG GTCACATGGT GGGCTTTAAG 
GAAGATCACT ATATGCTTCG GGAGAACCTG ATGGCCTCTG ACCACCTGGA CACGCCCATG 
CTACGAAGCG GGAACCTCAA GGGACGAGAC ACAGTCCGCT GGAAGATCAC CAACAATGTG 
CAGCGCCCTG GCTTTGCCAC CCATGCCGCC AGCACCAGCC CCACGGAGCT CGTACCCTAC 
GGGCTGTCCC TGCGCCTTGG CCGCCTCTGC ACTGAGAACC TTATGAAGCC GGGCACCCGA 
GAGTGTGACC AGCTACGCCA GGAGGTGGAG GAAAATCTGA ATGAGGTGTA TAGACAGGTC 
AGCGGCGCAC ACAAGCTCCA GCAGACGAAG TTCCGACAGC AGCCCAACAC CGGGAAAAAG 
CAAGACCACA CCATTGTGGA CACAGTGTTG CTGGCGCCCC GCTCCGCCAA GCAGATGCTG 
CTGAAGCTGA CAGAGAAGCA GGTGGAGCAG GGGTCCTTCC ATGAACTGAA GGTGGCCCCT 
GGCTACTACA CTGTCACGGC AGAGCAGGAT GCCCGGGGCA TGGTGGAGTT CCAGGAGGGC 
GTGGAGCTGG TGGATGTGCG AGTGCCCCTC TTCATCCGGC CTGAGGATGA TGATGAGAAG 
CAGCTGCTGG TGGAGGCCAT TGATGTCCCT GTGGGCACTG CCACCCTTGG TCGCCGTCTG 
GTAAACATCA CCATTATCAA GGAACAAGCT AGTGGGGTAG TGTCCTTCGA GCAGCCTGAA 
TACTCGGTGA GTCGTGGAGA CCAGGTGGCC CGCATCCCTG TCATCCGGCA CATCCTGGAC 
AATGGCAAGT CCCAGGTCTC CTATAGCACA CAGGATAATA CAGCACACGG ACACCGGGAT 
TATGTCCCTG TGGAGGGAGA GCTGCTGTTC CATCCTGGGG AGACCTGGAA GGAGTTGCAG 
GTGAAGCTAC TGGAGCTGCA GGAGGTCGAC TCCCTCCTGC GTGGCCGCCA GGTCCGCCGC 
TTCCAAGTCC AACTCAGCAA CCCCAAGTTC GGAGCCCGCC TGGGCCAGCC CAGCACAACC 
ACCGTTATTC TCGGTGAGCA CGATGAAACG GACAGGAGTC TCATAAATCA AACACTTTCA 
TCGCCTCCGC CACCCCATGG AGACCTGGGC GCCCCACAGA ACCCCAATGC CAAGGCTGCC 
GGGTCCAGGA AGATCCATTT CAACTGGCTG CCCCCTCCTG GCAAGCCAAT GGGGTACAGG 
GTGAAGTACT GGATCCAGGG CGACTCTGAG TCTGAAGCCC ACCTTCTAGA CAGCAAGGTG 
CCCTCAGTGG AACTCACCAA CCTGTATCCC TATTGCGACT ACGAAATGAA GGTGTGTGCC 
TATGGGGCAC AGGGTGAGGG GCCCTATAGC TCACTGGTGT CCTGCCGCAC CCACCAGGAA 
GTACCCAGCG AGCCAGGGAG GCTGGCTTTC AATGTAGTCT CTTCTACGGT GACGCAGCTG 
AGCTGGGCAG AGCCAGCTGA GACCAATGGC GAGATCACAG CCTACGAGGT CTGCTATGGA 
CTGGTCAATG AGGACAACCG ACCCATTGGA CCTATGAAGA AGGTGCTCGT GGACAACCCC 
AAGAACCGGA TGCTGCTCAT TGAGAATCTG CGAGAATCCC AGCCATACCG ATACACGGTT 
AAGGCGCGCA ATGGGGCAGG ATGGGGACCC GAGAGAGAGG CTATCATCAA CCTGGCTACA 
CAGCCCAAGC GGCCCATGTC CATCCCTATC ATCCCAGACA TCCCCATAGT GGACGCCCAG 
GGTGGAGAAG ACTACGAAAA CTTCCTTATG TACAGTGATG ACGTCCTGCG GTCCCCAGCC 
AGCAGCCAGA GGCCCAGCGT TTCTGATGAC ACTGGCTGCG GCTGGAAGTT CGAGCCTCTG 
CTCGGGGAGG AGCTGGACCT GCGGCGCGTC ACGTGGCGGC TACCCCCGGA ACTCATCCCG 
CGCCTGTCCG CCAGCAGCGG GCGCTCTGAC GAAGACGGCA GCGTTGCGGG GGGCGTGGAA 
GGGGAGGGCA GCGGCTGGAT CCGCGGGGCT ACGCCCCGAC CCCCGGGAGA GCACCTGGTG 
AATGGCCGGA TGGACTTTGC CTATCCAGGC AGCGCCAACT CCCTGCACAG AATGACTGCA 
GCCAATGTGG CCTATGGCAC GCATCTGAGC CCACACCTGT CCCACCGAGT GCTGAGCACG 
TCCTCCACCC TTACCCGGGA CTACCACTCT CTGACACGCA CAGAGCACTC CCACTCAGGC 
ACACTTCCCA GGGACTACTC CACCCTCACT TCCCTTTCCT CCCAAGATTC CCGTGGGGCT 
GTGGGTGTGC CTGACACACC CACTCGGCTG GTGTTCTCTG CCCTGGGGCC CACGTCTTTG 
AAGGTGAGCT GGCAGGAGCC ACAGTGTGAT CGGATGCTGC TGGGCTACAG TGTGGAATAC 
CAGCTACTAA ACGGCGGCGA GATGCATCGG CTCAACATCC CTAACCCTGG CCAAACCTCG 
GTGGTGGTAG AGGATCTCCT GCCTAACCAC TCCTATGTGT TCCGGGTACG GGCCCAGAGC 
CAGGAGGGCT GGGGCCGAGA GCGAGAGGGT GTCATCACCA TCGAGTCCCA GGTGCACCCG 
CAGAGCCCTC TCTGCCCCCT GCCAGGCTCA GCCTTCACTC TGAGCACCCC CAGCGCCCCG 
GGACCGCTGG TGTTCACTGC CCTAAGCCCA GACTCCCTGC AGCTCAGCTG GGAGCGGCCG 
AGGAGGCCCA ATGGAGATAT CCTTGGCTAC CTGGTGACCT GTGAGATGGC CCAAGGAGGA 
GCACCAGCCA GGACCTTCCG GGTGGACGGA GACAACCCTG AGAGCCGGTT GACTGTACCT 
GGCCTCAGTG AGAACGTTCC TTACAAGTTC AAGGTTCAGG CCAGGACGAC CGAGGGCTTC 
GGGCCAGAGC GTGAGGGTAT CATCACCATC GAGTCTCAGG TTGGAGGCCC CTTCCCACAG 
CTGGGCAGCC ATTCTGGGCT CTTCCAGAAC CCAGTGCAAA GCGAGTTCAG CAGCGTGACC 
AGCACGCACA GCACCACGAC TGAGCCCTTC CTCATGGATG GTCTAACCCT GGGGACCCAG 
CGCCTGGAAG CAGGAGGCTC CCTCACCCGG CATGTGACCC AGGAATTCGT GACCCGGACC 
TTAACGGCCA GTGGCTCTCT CAGCACTCAT ATGGACCAAC AGTTCTTCCA AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itgb4 ( XM_006532573.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532636.1 Mus musculus integrin beta 4 (Itgb4), transcript variant X5, mRNA.

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CloneID	OMu45809
Clone ID Related Accession (Same CDS sequence)	XM_006532573.4 , XM_006532573.3
Accession Version	XM_006532573.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	integrin beta-4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532573.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGC CCTGTTGCAG CCCATGGGTG AAGCTGCTGC TGCTGGCAGC AATGCTGAGT 
GCCAGCCTCC CTGGAGACCT GGCCAACCGC TGCAAGAAGG CTCAGGTGAA GAGCTGTACC 
GAGTGCATCC GGGTGGACAA GAGCTGTGCC TACTGCACAG ACGAGCTGTT CAAGGAGAGG 
CGCTGCAACA CCCAGGCGGA GCTTCTGGCT GCAGGCTGCA GGGGAGAGAG CATCCTGGTC 
ATGGAGAGCA GCCTTGAAAT CACAGAGAAC ACCCAGATCG ACACCAGCCT GCACCGCAGC 
CAGGTATCTC CCCAAGGCCT GCAAGTCCGG CTGCGGCCGG GTGAGGAGCG CAGCTTTGTG 
TTCCAGGTGT TTGAGCCCCT GGAGAGCCCC GTGGATCTGT ATATCCTCAT GGACTTCTCC 
AACTCCATGT CTGACGATCT GGACAACCTC AAGCAGATGG GGCAGAACCT GGCCAAGATC 
CTGCGCCAGC TCACCAGCGA CTACACCATT GGATTTGGAA AGTTTGTGGA CAAAGTCAGC 
GTCCCACAGA CAGACATGAG GCCCGAGAAA CTGAAGGAGC CCTGGCCCAA CAGTGATCCC 
CCGTTCTCCT TCAAGAACGT TATCAGCTTA ACGGAGAATG TGGAAGAATT CTGGAACAAA 
CTGCAAGGAG AACGCATCTC AGGCAACCTG GACGCTCCTG AAGGGGGCTT TGATGCCATC 
CTGCAGACAG CTGTGTGCAC AAGGGACATT GGCTGGAGGG CTGACAGCAC CCACCTGCTG 
GTGTTCTCCA CCGAGTCTGC CTTCCACTAC GAGGCTGATG GTGCCAACGT GCTGGCCGGC 
ATCATGAACC GCAATGATGA GAAATGCCAC CTGGACGCCT CGGGCGCCTA CACCCAATAC 
AAGACACAGG ACTACCCATC AGTGCCCACG CTGGTTCGCC TGCTTGCCAA GCATAACATC 
ATCCCCATCT TTGCTGTCAC CAACTACTCT TACAGCTACT ATGAGAAGCT CCATAAGTAT 
TTCCCCGTCT CCTCTCTGGG CGTCCTGCAG GAGGATTCAT CCAACATCGT GGAGCTGCTG 
GAGGAGGCCT TCTATCGAAT TCGCTCCAAC CTGGACATCC GGGCTCTGGA CAGCCCCAGA 
GGCCTGAGAA CAGAGGTCAC CTCCGATACT CTCCAGAAGA CGGAGACTGG GTCCTTTCAC 
ATCAAGCGGG GGGAAGTGGG CACATACAAT GTGCATCTCC GGGCAGTGGA GGACATAGAT 
GGGACACATG TGTGCCAGCT GGCTAAAGAA GACCAAGGGG GCAACATCCA CCTGAAACCC 
TCCTTCTCTG ATGGCCTCCG GATGGACGCG AGTGTGATCT GTGACGTGTG CCCCTGTGAG 
CTGCAAAAGG AAGTTCGATC AGCTCGCTGT CACTTCAGAG GAGACTTCAT GTGTGGACAC 
TGTGTGTGCA ATGAGGGCTG GAGTGGCAAA ACCTGCAACT GCTCCACCGG CTCTCTGAGT 
GACACACAGC CCTGCCTGCG TGAGGGTGAG GACAAACCGT GCTCGGGCCA CGGCGAGTGC 
CAGTGCGGAC GCTGTGTGTG CTATGGTGAA GGCCGCTACG AGGGTCACTT CTGCGAGTAT 
GACAACTTCC AGTGTCCCCG GACCTCTGGA TTCCTGTGCA ATGACCGGGG TCGCTGTTCT 
ATGGGAGAGT GTGTGTGTGA GCCTGGTTGG ACAGGCCGCA GCTGTGACTG TCCCCTCAGC 
AATGCCACCT GCATCGATAG CAACGGGGGC ATCTGCAACG GCCGAGGCTA CTGTGAGTGT 
GGCCGTTGTC ACTGCAACCA GCAGTCGCTC TACACGGACA CCACCTGTGA GATCAACTAC 
TCTGCGATAC GCCTGGGTCT CTGTGAGGAT CTCCGCTCCT GCGTACAGTG CCAGGCCTGG 
GGCACCGGGG AGAAGAAAGG GCGCGCGTGT GACGATTGCC CCTTTAAAGT CAAGATGGTA 
GACGAGCTTA AGAAAGCAGA AGAGGTGGTG GAGTACTGCT CCTTCCGGGA TGAGGATGAC 
GACTGCACTT ACAGCTACAA CGTGGAGGGC GATGGCAGCC CTGGGCCCAA CAGCACAGTC 
CTGGTCCACA AAAAGAAAGA CTGCCCTCCC GGCTCCTTCT GGTGGCTCAT CCCCCTGCTC 
ATCTTCCTCC TGTTGCTCCT GGCGTTGCTT CTGCTGCTCT GCTGGAAATA CTGTGCCTGC 
TGCAAAGCCT GCCTGGGGCT TCTTCCTTGC TGCAACCGAG GTCACATGGT GGGCTTTAAG 
GAAGATCACT ATATGCTTCG GGAGAACCTG ATGGCCTCTG ACCACCTGGA CACGCCCATG 
CTACGAAGCG GGAACCTCAA GGGACGAGAC ACAGTCCGCT GGAAGATCAC CAACAATGTG 
CAGCGCCCTG GCTTTGCCAC CCATGCCGCC AGCACCAGCC CCACGGAGCT CGTACCCTAC 
GGGCTGTCCC TGCGCCTTGG CCGCCTCTGC ACTGAGAACC TTATGAAGCC GGGCACCCGA 
GAGTGTGACC AGCTACGCCA GGAGGTGGAG GAAAATCTGA ATGAGGTGTA TAGACAGGTC 
AGCGGCGCAC ACAAGCTCCA GCAGACGAAG TTCCGACAGC AGCCCAACAC CGGGAAAAAG 
CAAGACCACA CCATTGTGGA CACAGTGTTG CTGGCGCCCC GCTCCGCCAA GCAGATGCTG 
CTGAAGCTGA CAGAGAAGCA GGTGGAGCAG GGGTCCTTCC ATGAACTGAA GGTGGCCCCT 
GGCTACTACA CTGTCACGGC AGAGCAGGAT GCCCGGGGCA TGGTGGAGTT CCAGGAGGGC 
GTGGAGCTGG TGGATGTGCG AGTGCCCCTC TTCATCCGGC CTGAGGATGA TGATGAGAAG 
CAGCTGCTGG TGGAGGCCAT TGATGTCCCT GTGGGCACTG CCACCCTTGG TCGCCGTCTG 
GTAAACATCA CCATTATCAA GGAACAAGCT AGTGGGGTAG TGTCCTTCGA GCAGCCTGAA 
TACTCGGTGA GTCGTGGAGA CCAGGTGGCC CGCATCCCTG TCATCCGGCA CATCCTGGAC 
AATGGCAAGT CCCAGGTCTC CTATAGCACA CAGGATAATA CAGCACACGG ACACCGGGAT 
TATGTCCCTG TGGAGGGAGA GCTGCTGTTC CATCCTGGGG AGACCTGGAA GGAGTTGCAG 
GTGAAGCTAC TGGAGCTGCA GGAGGTCGAC TCCCTCCTGC GTGGCCGCCA GGTCCGCCGC 
TTCCAAGTCC AACTCAGCAA CCCCAAGTTC GGAGCCCGCC TGGGCCAGCC CAGCACAACC 
ACCGTTATTC TCGGTGAGCA CGATGAAACG GACAGGAGTC TCATAAATCA AACACTTTCA 
TCGCCTCCGC CACCCCATGG AGACCTGGGC GCCCCACAGA ACCCCAATGC CAAGGCTGCC 
GGGTCCAGGA AGATCCATTT CAACTGGCTG CCCCCTCCTG GCAAGCCAAT GGGGTACAGG 
GTGAAGTACT GGATCCAGGG CGACTCTGAG TCTGAAGCCC ACCTTCTAGA CAGCAAGGTG 
CCCTCAGTGG AACTCACCAA CCTGTATCCC TATTGCGACT ACGAAATGAA GGTGTGTGCC 
TATGGGGCAC AGGGTGAGGG GCCCTATAGC TCACTGGTGT CCTGCCGCAC CCACCAGGAA 
GTACCCAGCG AGCCAGGGAG GCTGGCTTTC AATGTAGTCT CTTCTACGGT GACGCAGCTG 
AGCTGGGCAG AGCCAGCTGA GACCAATGGC GAGATCACAG CCTACGAGGT CTGCTATGGA 
CTGGTCAATG AGGACAACCG ACCCATTGGA CCTATGAAGA AGGTGCTCGT GGACAACCCC 
AAGAACCGGA TGCTGCTCAT TGAGAATCTG CGAGAATCCC AGCCATACCG ATACACGGTT 
AAGGCGCGCA ATGGGGCAGG ATGGGGACCC GAGAGAGAGG CTATCATCAA CCTGGCTACA 
CAGCCCAAGC GGCCCATGTC CATCCCTATC ATCCCAGACA TCCCCATAGT GGACGCCCAG 
GGTGGAGAAG ACTACGAAAA CTTCCTTATG TACAGTGATG ACGTCCTGCG GTCCCCAGCC 
AGCAGCCAGA GGCCCAGCGT TTCTGATGAC ACTGAGCACC TGGTGAATGG CCGGATGGAC 
TTTGCCTATC CAGGCAGCGC CAACTCCCTG CACAGAATGA CTGCAGCCAA TGTGGCCTAT 
GGCACGCATC TGAGCCCACA CCTGTCCCAC CGAGTGCTGA GCACGTCCTC CACCCTTACC 
CGGGACTACC ACTCTCTGAC ACGCACAGAG CACTCCCACT CAGGCACACT TCCCAGGGAC 
TACTCCACCC TCACTTCCCT TTCCTCCCAA GGCCTCCCTC CTATCTGGGA AGATGGGAGG 
AGCAGGCTTC CGCTGTCCTG GACTCTTGGG TCCTTGAGCC GGGCTCACAT GAAGGGTGTG 
CCCGCATCCA GGGGTTCACC AGACTCTATA ATCCTGGCCG GGCAGTCAGC AGCACCCTCC 
TGGGGTACAG ATTCCCGTGG GGCTGTGGGT GTGCCTGACA CACCCACTCG GCTGGTGTTC 
TCTGCCCTGG GGCCCACGTC TTTGAAGGTG AGCTGGCAGG AGCCACAGTG TGATCGGATG 
CTGCTGGGCT ACAGTGTGGA ATACCAGCTA CTAAACGGCG GCGAGATGCA TCGGCTCAAC 
ATCCCTAACC CTGGCCAAAC CTCGGTGGTG GTAGAGGATC TCCTGCCTAA CCACTCCTAT 
GTGTTCCGGG TACGGGCCCA GAGCCAGGAG GGCTGGGGCC GAGAGCGAGA GGGTGTCATC 
ACCATCGAGT CCCAGGTGCA CCCGCAGAGC CCTCTCTGCC CCCTGCCAGG CTCAGCCTTC 
ACTCTGAGCA CCCCCAGCGC CCCGGGACCG CTGGTGTTCA CTGCCCTAAG CCCAGACTCC 
CTGCAGCTCA GCTGGGAGCG GCCGAGGAGG CCCAATGGAG ATATCCTTGG CTACCTGGTG 
ACCTGTGAGA TGGCCCAAGG AGGAGCACCA GCCAGGACCT TCCGGGTGGA CGGAGACAAC 
CCTGAGAGCC GGTTGACTGT ACCTGGCCTC AGTGAGAACG TTCCTTACAA GTTCAAGGTT 
CAGGCCAGGA CGACCGAGGG CTTCGGGCCA GAGCGTGAGG GTATCATCAC CATCGAGTCT 
CAGGTTGGAG GCCCCTTCCC ACAGCTGGGC AGCCATTCTG GGCTCTTCCA GAACCCAGTG 
CAAAGCGAGT TCAGCAGCGT GACCAGCACG CACAGCACCA CGACTGAGCC CTTCCTCATG 
GATGGTCTAA CCCTGGGGAC CCAGCGCCTG GAAGCAGGAG GCTCCCTCAC CCGGCATGTG 
ACCCAGGAAT TCGTGACCCG GACCTTAACG GCCAGTGGCT CTCTCAGCAC TCATATGGAC 
CAACAGTTCT TCCAAACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532636.1
CDS	436..5856
Translation	MAGPCCSPWVKLLLLAAMLSASLPGDLANRCKKAQVKSCTECIRVDKSCAYCTDELFKERRCNTQAELLAAGCRGESILVMESSLEITENTQIDTSLHRSQVSPQGLQVRLRPGEERSFVFQVFEPLESPVDLYILMDFSNSMSDDLDNLKQMGQNLAKILRQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTENVEEFWNKLQGERISGNLDAPEGGFDAILQTAVCTRDIGWRADSTHLLVFSTESAFHYEADGANVLAGIMNRNDEKCHLDASGAYTQYKTQDYPSVPTLVRLLAKHNIIPIFAVTNYSYSYYEKLHKYFPVSSLGVLQEDSSNIVELLEEAFYRIRSNLDIRALDSPRGLRTEVTSDTLQKTETGSFHIKRGEVGTYNVHLRAVEDIDGTHVCQLAKEDQGGNIHLKPSFSDGLRMDASVICDVCPCELQKEVRSARCHFRGDFMCGHCVCNEGWSGKTCNCSTGSLSDTQPCLREGEDKPCSGHGECQCGRCVCYGEGRYEGHFCEYDNFQCPRTSGFLCNDRGRCSMGECVCEPGWTGRSCDCPLSNATCIDSNGGICNGRGYCECGRCHCNQQSLYTDTTCEINYSAIRLGLCEDLRSCVQCQAWGTGEKKGRACDDCPFKVKMVDELKKAEEVVEYCSFRDEDDDCTYSYNVEGDGSPGPNSTVLVHKKKDCPPGSFWWLIPLLIFLLLLLALLLLLCWKYCACCKACLGLLPCCNRGHMVGFKEDHYMLRENLMASDHLDTPMLRSGNLKGRDTVRWKITNNVQRPGFATHAASTSPTELVPYGLSLRLGRLCTENLMKPGTRECDQLRQEVEENLNEVYRQVSGAHKLQQTKFRQQPNTGKKQDHTIVDTVLLAPRSAKQMLLKLTEKQVEQGSFHELKVAPGYYTVTAEQDARGMVEFQEGVELVDVRVPLFIRPEDDDEKQLLVEAIDVPVGTATLGRRLVNITIIKEQASGVVSFEQPEYSVSRGDQVARIPVIRHILDNGKSQVSYSTQDNTAHGHRDYVPVEGELLFHPGETWKELQVKLLELQEVDSLLRGRQVRRFQVQLSNPKFGARLGQPSTTTVILGEHDETDRSLINQTLSSPPPPHGDLGAPQNPNAKAAGSRKIHFNWLPPPGKPMGYRVKYWIQGDSESEAHLLDSKVPSVELTNLYPYCDYEMKVCAYGAQGEGPYSSLVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYGLVNEDNRPIGPMKKVLVDNPKNRMLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDIPIVDAQGGEDYENFLMYSDDVLRSPASSQRPSVSDDTEHLVNGRMDFAYPGSANSLHRMTAANVAYGTHLSPHLSHRVLSTSSTLTRDYHSLTRTEHSHSGTLPRDYSTLTSLSSQGLPPIWEDGRSRLPLSWTLGSLSRAHMKGVPASRGSPDSIILAGQSAAPSWGTDSRGAVGVPDTPTRLVFSALGPTSLKVSWQEPQCDRMLLGYSVEYQLLNGGEMHRLNIPNPGQTSVVVEDLLPNHSYVFRVRAQSQEGWGREREGVITIESQVHPQSPLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPNGDILGYLVTCEMAQGGAPARTFRVDGDNPESRLTVPGLSENVPYKFKVQARTTEGFGPEREGIITIESQVGGPFPQLGSHSGLFQNPVQSEFSSVTSTHSTTTEPFLMDGLTLGTQRLEAGGSLTRHVTQEFVTRTLTASGSLSTHMDQQFFQT

Target ORF information:

RefSeq Version	XM_006532573.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus integrin beta 4 (Itgb4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532573.4

ORF Insert Sequence:

ATGGCAGGGC CCTGTTGCAG CCCATGGGTG AAGCTGCTGC TGCTGGCAGC AATGCTGAGT 
GCCAGCCTCC CTGGAGACCT GGCCAACCGC TGCAAGAAGG CTCAGGTGAA GAGCTGTACC 
GAGTGCATCC GGGTGGACAA GAGCTGTGCC TACTGCACAG ACGAGCTGTT CAAGGAGAGG 
CGCTGCAACA CCCAGGCGGA GCTTCTGGCT GCAGGCTGCA GGGGAGAGAG CATCCTGGTC 
ATGGAGAGCA GCCTTGAAAT CACAGAGAAC ACCCAGATCG ACACCAGCCT GCACCGCAGC 
CAGGTATCTC CCCAAGGCCT GCAAGTCCGG CTGCGGCCGG GTGAGGAGCG CAGCTTTGTG 
TTCCAGGTGT TTGAGCCCCT GGAGAGCCCC GTGGATCTGT ATATCCTCAT GGACTTCTCC 
AACTCCATGT CTGACGATCT GGACAACCTC AAGCAGATGG GGCAGAACCT GGCCAAGATC 
CTGCGCCAGC TCACCAGCGA CTACACCATT GGATTTGGAA AGTTTGTGGA CAAAGTCAGC 
GTCCCACAGA CAGACATGAG GCCCGAGAAA CTGAAGGAGC CCTGGCCCAA CAGTGATCCC 
CCGTTCTCCT TCAAGAACGT TATCAGCTTA ACGGAGAATG TGGAAGAATT CTGGAACAAA 
CTGCAAGGAG AACGCATCTC AGGCAACCTG GACGCTCCTG AAGGGGGCTT TGATGCCATC 
CTGCAGACAG CTGTGTGCAC AAGGGACATT GGCTGGAGGG CTGACAGCAC CCACCTGCTG 
GTGTTCTCCA CCGAGTCTGC CTTCCACTAC GAGGCTGATG GTGCCAACGT GCTGGCCGGC 
ATCATGAACC GCAATGATGA GAAATGCCAC CTGGACGCCT CGGGCGCCTA CACCCAATAC 
AAGACACAGG ACTACCCATC AGTGCCCACG CTGGTTCGCC TGCTTGCCAA GCATAACATC 
ATCCCCATCT TTGCTGTCAC CAACTACTCT TACAGCTACT ATGAGAAGCT CCATAAGTAT 
TTCCCCGTCT CCTCTCTGGG CGTCCTGCAG GAGGATTCAT CCAACATCGT GGAGCTGCTG 
GAGGAGGCCT TCTATCGAAT TCGCTCCAAC CTGGACATCC GGGCTCTGGA CAGCCCCAGA 
GGCCTGAGAA CAGAGGTCAC CTCCGATACT CTCCAGAAGA CGGAGACTGG GTCCTTTCAC 
ATCAAGCGGG GGGAAGTGGG CACATACAAT GTGCATCTCC GGGCAGTGGA GGACATAGAT 
GGGACACATG TGTGCCAGCT GGCTAAAGAA GACCAAGGGG GCAACATCCA CCTGAAACCC 
TCCTTCTCTG ATGGCCTCCG GATGGACGCG AGTGTGATCT GTGACGTGTG CCCCTGTGAG 
CTGCAAAAGG AAGTTCGATC AGCTCGCTGT CACTTCAGAG GAGACTTCAT GTGTGGACAC 
TGTGTGTGCA ATGAGGGCTG GAGTGGCAAA ACCTGCAACT GCTCCACCGG CTCTCTGAGT 
GACACACAGC CCTGCCTGCG TGAGGGTGAG GACAAACCGT GCTCGGGCCA CGGCGAGTGC 
CAGTGCGGAC GCTGTGTGTG CTATGGTGAA GGCCGCTACG AGGGTCACTT CTGCGAGTAT 
GACAACTTCC AGTGTCCCCG GACCTCTGGA TTCCTGTGCA ATGACCGGGG TCGCTGTTCT 
ATGGGAGAGT GTGTGTGTGA GCCTGGTTGG ACAGGCCGCA GCTGTGACTG TCCCCTCAGC 
AATGCCACCT GCATCGATAG CAACGGGGGC ATCTGCAACG GCCGAGGCTA CTGTGAGTGT 
GGCCGTTGTC ACTGCAACCA GCAGTCGCTC TACACGGACA CCACCTGTGA GATCAACTAC 
TCTGCGATAC GCCTGGGTCT CTGTGAGGAT CTCCGCTCCT GCGTACAGTG CCAGGCCTGG 
GGCACCGGGG AGAAGAAAGG GCGCGCGTGT GACGATTGCC CCTTTAAAGT CAAGATGGTA 
GACGAGCTTA AGAAAGCAGA AGAGGTGGTG GAGTACTGCT CCTTCCGGGA TGAGGATGAC 
GACTGCACTT ACAGCTACAA CGTGGAGGGC GATGGCAGCC CTGGGCCCAA CAGCACAGTC 
CTGGTCCACA AAAAGAAAGA CTGCCCTCCC GGCTCCTTCT GGTGGCTCAT CCCCCTGCTC 
ATCTTCCTCC TGTTGCTCCT GGCGTTGCTT CTGCTGCTCT GCTGGAAATA CTGTGCCTGC 
TGCAAAGCCT GCCTGGGGCT TCTTCCTTGC TGCAACCGAG GTCACATGGT GGGCTTTAAG 
GAAGATCACT ATATGCTTCG GGAGAACCTG ATGGCCTCTG ACCACCTGGA CACGCCCATG 
CTACGAAGCG GGAACCTCAA GGGACGAGAC ACAGTCCGCT GGAAGATCAC CAACAATGTG 
CAGCGCCCTG GCTTTGCCAC CCATGCCGCC AGCACCAGCC CCACGGAGCT CGTACCCTAC 
GGGCTGTCCC TGCGCCTTGG CCGCCTCTGC ACTGAGAACC TTATGAAGCC GGGCACCCGA 
GAGTGTGACC AGCTACGCCA GGAGGTGGAG GAAAATCTGA ATGAGGTGTA TAGACAGGTC 
AGCGGCGCAC ACAAGCTCCA GCAGACGAAG TTCCGACAGC AGCCCAACAC CGGGAAAAAG 
CAAGACCACA CCATTGTGGA CACAGTGTTG CTGGCGCCCC GCTCCGCCAA GCAGATGCTG 
CTGAAGCTGA CAGAGAAGCA GGTGGAGCAG GGGTCCTTCC ATGAACTGAA GGTGGCCCCT 
GGCTACTACA CTGTCACGGC AGAGCAGGAT GCCCGGGGCA TGGTGGAGTT CCAGGAGGGC 
GTGGAGCTGG TGGATGTGCG AGTGCCCCTC TTCATCCGGC CTGAGGATGA TGATGAGAAG 
CAGCTGCTGG TGGAGGCCAT TGATGTCCCT GTGGGCACTG CCACCCTTGG TCGCCGTCTG 
GTAAACATCA CCATTATCAA GGAACAAGCT AGTGGGGTAG TGTCCTTCGA GCAGCCTGAA 
TACTCGGTGA GTCGTGGAGA CCAGGTGGCC CGCATCCCTG TCATCCGGCA CATCCTGGAC 
AATGGCAAGT CCCAGGTCTC CTATAGCACA CAGGATAATA CAGCACACGG ACACCGGGAT 
TATGTCCCTG TGGAGGGAGA GCTGCTGTTC CATCCTGGGG AGACCTGGAA GGAGTTGCAG 
GTGAAGCTAC TGGAGCTGCA GGAGGTCGAC TCCCTCCTGC GTGGCCGCCA GGTCCGCCGC 
TTCCAAGTCC AACTCAGCAA CCCCAAGTTC GGAGCCCGCC TGGGCCAGCC CAGCACAACC 
ACCGTTATTC TCGGTGAGCA CGATGAAACG GACAGGAGTC TCATAAATCA AACACTTTCA 
TCGCCTCCGC CACCCCATGG AGACCTGGGC GCCCCACAGA ACCCCAATGC CAAGGCTGCC 
GGGTCCAGGA AGATCCATTT CAACTGGCTG CCCCCTCCTG GCAAGCCAAT GGGGTACAGG 
GTGAAGTACT GGATCCAGGG CGACTCTGAG TCTGAAGCCC ACCTTCTAGA CAGCAAGGTG 
CCCTCAGTGG AACTCACCAA CCTGTATCCC TATTGCGACT ACGAAATGAA GGTGTGTGCC 
TATGGGGCAC AGGGTGAGGG GCCCTATAGC TCACTGGTGT CCTGCCGCAC CCACCAGGAA 
GTACCCAGCG AGCCAGGGAG GCTGGCTTTC AATGTAGTCT CTTCTACGGT GACGCAGCTG 
AGCTGGGCAG AGCCAGCTGA GACCAATGGC GAGATCACAG CCTACGAGGT CTGCTATGGA 
CTGGTCAATG AGGACAACCG ACCCATTGGA CCTATGAAGA AGGTGCTCGT GGACAACCCC 
AAGAACCGGA TGCTGCTCAT TGAGAATCTG CGAGAATCCC AGCCATACCG ATACACGGTT 
AAGGCGCGCA ATGGGGCAGG ATGGGGACCC GAGAGAGAGG CTATCATCAA CCTGGCTACA 
CAGCCCAAGC GGCCCATGTC CATCCCTATC ATCCCAGACA TCCCCATAGT GGACGCCCAG 
GGTGGAGAAG ACTACGAAAA CTTCCTTATG TACAGTGATG ACGTCCTGCG GTCCCCAGCC 
AGCAGCCAGA GGCCCAGCGT TTCTGATGAC ACTGAGCACC TGGTGAATGG CCGGATGGAC 
TTTGCCTATC CAGGCAGCGC CAACTCCCTG CACAGAATGA CTGCAGCCAA TGTGGCCTAT 
GGCACGCATC TGAGCCCACA CCTGTCCCAC CGAGTGCTGA GCACGTCCTC CACCCTTACC 
CGGGACTACC ACTCTCTGAC ACGCACAGAG CACTCCCACT CAGGCACACT TCCCAGGGAC 
TACTCCACCC TCACTTCCCT TTCCTCCCAA GGCCTCCCTC CTATCTGGGA AGATGGGAGG 
AGCAGGCTTC CGCTGTCCTG GACTCTTGGG TCCTTGAGCC GGGCTCACAT GAAGGGTGTG 
CCCGCATCCA GGGGTTCACC AGACTCTATA ATCCTGGCCG GGCAGTCAGC AGCACCCTCC 
TGGGGTACAG ATTCCCGTGG GGCTGTGGGT GTGCCTGACA CACCCACTCG GCTGGTGTTC 
TCTGCCCTGG GGCCCACGTC TTTGAAGGTG AGCTGGCAGG AGCCACAGTG TGATCGGATG 
CTGCTGGGCT ACAGTGTGGA ATACCAGCTA CTAAACGGCG GCGAGATGCA TCGGCTCAAC 
ATCCCTAACC CTGGCCAAAC CTCGGTGGTG GTAGAGGATC TCCTGCCTAA CCACTCCTAT 
GTGTTCCGGG TACGGGCCCA GAGCCAGGAG GGCTGGGGCC GAGAGCGAGA GGGTGTCATC 
ACCATCGAGT CCCAGGTGCA CCCGCAGAGC CCTCTCTGCC CCCTGCCAGG CTCAGCCTTC 
ACTCTGAGCA CCCCCAGCGC CCCGGGACCG CTGGTGTTCA CTGCCCTAAG CCCAGACTCC 
CTGCAGCTCA GCTGGGAGCG GCCGAGGAGG CCCAATGGAG ATATCCTTGG CTACCTGGTG 
ACCTGTGAGA TGGCCCAAGG AGGAGCACCA GCCAGGACCT TCCGGGTGGA CGGAGACAAC 
CCTGAGAGCC GGTTGACTGT ACCTGGCCTC AGTGAGAACG TTCCTTACAA GTTCAAGGTT 
CAGGCCAGGA CGACCGAGGG CTTCGGGCCA GAGCGTGAGG GTATCATCAC CATCGAGTCT 
CAGGTTGGAG GCCCCTTCCC ACAGCTGGGC AGCCATTCTG GGCTCTTCCA GAACCCAGTG 
CAAAGCGAGT TCAGCAGCGT GACCAGCACG CACAGCACCA CGACTGAGCC CTTCCTCATG 
GATGGTCTAA CCCTGGGGAC CCAGCGCCTG GAAGCAGGAG GCTCCCTCAC CCGGCATGTG 
ACCCAGGAAT TCGTGACCCG GACCTTAACG GCCAGTGGCT CTCTCAGCAC TCATATGGAC 
CAACAGTTCT TCCAAACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itgb4 ( XM_017314349.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169838.1 Mus musculus integrin beta 4 (Itgb4), transcript variant X1, mRNA.

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CloneID	OMu77781
Clone ID Related Accession (Same CDS sequence)	XM_017314349.2 , XM_017314349.1
Accession Version	XM_017314349.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	integrin beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314349.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGC CCTGTTGCAG CCCATGGGTG AAGCTGCTGC TGCTGGCAGC AATGCTGAGT 
GCCAGCCTCC CTGGAGACCT GGCCAACCGC TGCAAGAAGG CTCAGGTGAA GAGCTGTACC 
GAGTGCATCC GGGTGGACAA GAGCTGTGCC TACTGCACAG ACGAGCTGTT CAAGGAGAGG 
CGCTGCAACA CCCAGGCGGA GCTTCTGGCT GCAGGCTGCA GGGGAGAGAG CATCCTGGTC 
ATGGAGAGCA GCCTTGAAAT CACAGAGAAC ACCCAGATCG ACACCAGCCT GCACCGCAGC 
CAGGTATCTC CCCAAGGCCT GCAAGTCCGG CTGCGGCCGG GTGAGGAGCG CAGCTTTGTG 
TTCCAGGTGT TTGAGCCCCT GGAGAGCCCC GTGGATCTGT ATATCCTCAT GGACTTCTCC 
AACTCCATGT CTGACGATCT GGACAACCTC AAGCAGATGG GGCAGAACCT GGCCAAGATC 
CTGCGCCAGC TCACCAGCGA CTACACCATT GGATTTGGAA AGTTTGTGGA CAAAGTCAGC 
GTCCCACAGA CAGACATGAG GCCCGAGAAA CTGAAGGAGC CCTGGCCCAA CAGTGATCCC 
CCGTTCTCCT TCAAGAACGT TATCAGCTTA ACGGAGAATG TGGAAGAATT CTGGAACAAA 
CTGCAAGGAG AACGCATCTC AGGCAACCTG GACGCTCCTG AAGGGGGCTT TGATGCCATC 
CTGCAGACAG CTGTGTGCAC AAGGGACATT GGCTGGAGGG CTGACAGCAC CCACCTGCTG 
GTGTTCTCCA CCGAGTCTGC CTTCCACTAC GAGGCTGATG GTGCCAACGT GCTGGCCGGC 
ATCATGAACC GCAATGATGA GAAATGCCAC CTGGACGCCT CGGGCGCCTA CACCCAATAC 
AAGACACAGG ACTACCCATC AGTGCCCACG CTGGTTCGCC TGCTTGCCAA GCATAACATC 
ATCCCCATCT TTGCTGTCAC CAACTACTCT TACAGCTACT ATGAGAAGCT CCATAAGTAT 
TTCCCCGTCT CCTCTCTGGG CGTCCTGCAG GAGGATTCAT CCAACATCGT GGAGCTGCTG 
GAGGAGGCCT TCTATCGAAT TCGCTCCAAC CTGGACATCC GGGCTCTGGA CAGCCCCAGA 
GGCCTGAGAA CAGAGGTCAC CTCCGATACT CTCCAGAAGA CGGAGACTGG GTCCTTTCAC 
ATCAAGCGGG GGGAAGTGGG CACATACAAT GTGCATCTCC GGGCAGTGGA GGACATAGAT 
GGGACACATG TGTGCCAGCT GGCTAAAGAA GACCAAGGGG GCAACATCCA CCTGAAACCC 
TCCTTCTCTG ATGGCCTCCG GATGGACGCG AGTGTGATCT GTGACGTGTG CCCCTGTGAG 
CTGCAAAAGG AAGTTCGATC AGCTCGCTGT CACTTCAGAG GAGACTTCAT GTGTGGACAC 
TGTGTGTGCA ATGAGGGCTG GAGTGGCAAA ACCTGCAACT GCTCCACCGG CTCTCTGAGT 
GACACACAGC CCTGCCTGCG TGAGGGTGAG GACAAACCGT GCTCGGGCCA CGGCGAGTGC 
CAGTGCGGAC GCTGTGTGTG CTATGGTGAA GGCCGCTACG AGGGTCACTT CTGCGAGTAT 
GACAACTTCC AGTGTCCCCG GACCTCTGGA TTCCTGTGCA ATGACCGGGG TCGCTGTTCT 
ATGGGAGAGT GTGTGTGTGA GCCTGGTTGG ACAGGCCGCA GCTGTGACTG TCCCCTCAGC 
AATGCCACCT GCATCGATAG CAACGGGGGC ATCTGCAACG GCCGAGGCTA CTGTGAGTGT 
GGCCGTTGTC ACTGCAACCA GCAGTCGCTC TACACGGACA CCACCTGTGA GATCAACTAC 
TCTGCGATAC GCCTGGGTCT CTGTGAGGAT CTCCGCTCCT GCGTACAGTG CCAGGCCTGG 
GGCACCGGGG AGAAGAAAGG GCGCGCGTGT GACGATTGCC CCTTTAAAGT CAAGATGGTA 
GACGAGCTTA AGAAAGCAGA AGAGGTGGTG GAGTACTGCT CCTTCCGGGA TGAGGATGAC 
GACTGCACTT ACAGCTACAA CGTGGAGGGC GATGGCAGCC CTGGGCCCAA CAGCACAGTC 
CTGGTCCACA AAAAGAAAGA CTGCCCTCCC GGCTCCTTCT GGTGGCTCAT CCCCCTGCTC 
ATCTTCCTCC TGTTGCTCCT GGCGTTGCTT CTGCTGCTCT GCTGGAAATA CTGTGCCTGC 
TGCAAAGCCT GCCTGGGGCT TCTTCCTTGC TGCAACCGAG GTCACATGGT GGGCTTTAAG 
GAAGATCACT ATATGCTTCG GGAGAACCTG ATGGCCTCTG ACCACCTGGA CACGCCCATG 
CTACGAAGCG GGAACCTCAA GGGACGAGAC ACAGTCCGCT GGAAGATCAC CAACAATGTG 
CAGCGCCCTG GCTTTGCCAC CCATGCCGCC AGCACCAGCC CCACGGAGCT CGTACCCTAC 
GGGCTGTCCC TGCGCCTTGG CCGCCTCTGC ACTGAGAACC TTATGAAGCC GGGCACCCGA 
GAGTGTGACC AGCTACGCCA GGAGGTGGAG GAAAATCTGA ATGAGGTGTA TAGACAGGTC 
AGCGGCGCAC ACAAGCTCCA GCAGACGAAG TTCCGACAGC AGCCCAACAC CGGGAAAAAG 
CAAGACCACA CCATTGTGGA CACAGTGTTG CTGGCGCCCC GCTCCGCCAA GCAGATGCTG 
CTGAAGCTGA CAGAGAAGCA GGTGGAGCAG GGGTCCTTCC ATGAACTGAA GGTGGCCCCT 
GGCTACTACA CTGTCACGGC AGAGCAGGAT GCCCGGGGCA TGGTGGAGTT CCAGGAGGGC 
GTGGAGCTGG TGGATGTGCG AGTGCCCCTC TTCATCCGGC CTGAGGATGA TGATGAGAAG 
CAGCTGCTGG TGGAGGCCAT TGATGTCCCT GTGGGCACTG CCACCCTTGG TCGCCGTCTG 
GTAAACATCA CCATTATCAA GGAACAAGCT AGTGGGGTAG TGTCCTTCGA GCAGCCTGAA 
TACTCGGTGA GTCGTGGAGA CCAGGTGGCC CGCATCCCTG TCATCCGGCA CATCCTGGAC 
AATGGCAAGT CCCAGGTCTC CTATAGCACA CAGGATAATA CAGCACACGG ACACCGGGAT 
TATGTCCCTG TGGAGGGAGA GCTGCTGTTC CATCCTGGGG AGACCTGGAA GGAGTTGCAG 
GTGAAGCTAC TGGAGCTGCA GGAGGTCGAC TCCCTCCTGC GTGGCCGCCA GGTCCGCCGC 
TTCCAAGTCC AACTCAGCAA CCCCAAGTTC GGAGCCCGCC TGGGCCAGCC CAGCACAACC 
ACCGTTATTC TCGGTGAGCA CGATGAAACG GACAGGAGTC TCATAAATCA AACACTTTCA 
TCGCCTCCGC CACCCCATGG AGACCTGGGC GCCCCACAGA ACCCCAATGC CAAGGCTGCC 
GGGTCCAGGA AGATCCATTT CAACTGGCTG CCCCCTCCTG GCAAGCCAAT GGGGTACAGG 
GTGAAGTACT GGATCCAGGG CGACTCTGAG TCTGAAGCCC ACCTTCTAGA CAGCAAGGTG 
CCCTCAGTGG AACTCACCAA CCTGTATCCC TATTGCGACT ACGAAATGAA GGTGTGTGCC 
TATGGGGCAC AGGGTGAGGG GCCCTATAGC TCACTGGTGT CCTGCCGCAC CCACCAGGAA 
GTACCCAGCG AGCCAGGGAG GCTGGCTTTC AATGTAGTCT CTTCTACGGT GACGCAGCTG 
AGCTGGGCAG AGCCAGCTGA GACCAATGGC GAGATCACAG CCTACGAGGT CTGCTATGGA 
CTGGTCAATG AGGACAACCG ACCCATTGGA CCTATGAAGA AGGTGCTCGT GGACAACCCC 
AAGAACCGGA TGCTGCTCAT TGAGAATCTG CGAGAATCCC AGCCATACCG ATACACGGTT 
AAGGCGCGCA ATGGGGCAGG ATGGGGACCC GAGAGAGAGG CTATCATCAA CCTGGCTACA 
CAGCCCAAGC GGCCCATGTC CATCCCTATC ATCCCAGACA TCCCCATAGT GGACGCCCAG 
GGTGGAGAAG ACTACGAAAA CTTCCTTATG TACAGTGATG ACGTCCTGCG GTCCCCAGCC 
AGCAGCCAGA GGCCCAGCGT TTCTGATGAC ACTGGCTGCG GCTGGAAGTT CGAGCCTCTG 
CTCGGGGAGG AGCTGGACCT GCGGCGCGTC ACGTGGCGGC TACCCCCGGA ACTCATCCCG 
CGCCTGTCCG CCAGCAGCGG GCGCTCTGAC GAAGACGGCA GCGTTGCGGG GGGCGTGGAA 
GGGGAGGGCA GCGGCTGGAT CCGCGGGGCT ACGCCCCGAC CCCCGGGAGA GCACCTGGTG 
AATGGCCGGA TGGACTTTGC CTATCCAGGC AGCGCCAACT CCCTGCACAG AATGACTGCA 
GCCAATGTGG CCTATGGCAC GCATCTGAGC CCACACCTGT CCCACCGAGT GCTGAGCACG 
TCCTCCACCC TTACCCGGGA CTACCACTCT CTGACACGCA CAGAGCACTC CCACTCAGGC 
ACACTTCCCA GGGACTACTC CACCCTCACT TCCCTTTCCT CCCAAGGCCT CCCTCCTATC 
TGGGAAGATG GGAGGAGCAG GCTTCCGCTG TCCTGGACTC TTGGGTCCTT GAGCCGGGCT 
CACATGAAGG GTGTGCCCGC ATCCAGGGGT TCACCAGACT CTATAATCCT GGCCGGGCAG 
TCAGCAGCAC CCTCCTGGGG TACAGATTCC CGTGGGGCTG TGGGTGTGCC TGACACACCC 
ACTCGGCTGG TGTTCTCTGC CCTGGGGCCC ACGTCTTTGA AGGTGAGCTG GCAGGAGCCA 
CAGTGTGATC GGATGCTGCT GGGCTACAGT GTGGAATACC AGCTACTAAA CGGCGGCGAG 
ATGCATCGGC TCAACATCCC TAACCCTGGC CAAACCTCGG TGGTGGTAGA GGATCTCCTG 
CCTAACCACT CCTATGTGTT CCGGGTACGG GCCCAGAGCC AGGAGGGCTG GGGCCGAGAG 
CGAGAGGGTG TCATCACCAT CGAGTCCCAG GTGCACCCGC AGAGCCCTCT CTGCCCCCTG 
CCAGGCTCAG CCTTCACTCT GAGCACCCCC AGCGCCCCGG GACCGCTGGT GTTCACTGCC 
CTAAGCCCAG ACTCCCTGCA GCTCAGCTGG GAGCGGCCGA GGAGGCCCAA TGGAGATATC 
CTTGGCTACC TGGTGACCTG TGAGATGGCC CAAGGAGGAG CACCAGCCAG GACCTTCCGG 
GTGGACGGAG ACAACCCTGA GAGCCGGTTG ACTGTACCTG GCCTCAGTGA GAACGTTCCT 
TACAAGTTCA AGGTTCAGGC CAGGACGACC GAGGGCTTCG GGCCAGAGCG TGAGGGTATC 
ATCACCATCG AGTCTCAGGT TGGAGGCCCC TTCCCACAGC TGGGCAGCCA TTCTGGGCTC 
TTCCAGAACC CAGTGCAAAG CGAGTTCAGC AGCGTGACCA GCACGCACAG CACCACGACT 
GAGCCCTTCC TCATGGATGG TCTAACCCTG GGGACCCAGC GCCTGGAAGC AGGAGGCTCC 
CTCACCCGGC ATGTGACCCA GGAATTCGTG ACCCGGACCT TAACGGCCAG TGGCTCTCTC 
AGCACTCATA TGGACCAACA GTTCTTCCAA ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169838.1
CDS	436..6051
Translation	MAGPCCSPWVKLLLLAAMLSASLPGDLANRCKKAQVKSCTECIRVDKSCAYCTDELFKERRCNTQAELLAAGCRGESILVMESSLEITENTQIDTSLHRSQVSPQGLQVRLRPGEERSFVFQVFEPLESPVDLYILMDFSNSMSDDLDNLKQMGQNLAKILRQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTENVEEFWNKLQGERISGNLDAPEGGFDAILQTAVCTRDIGWRADSTHLLVFSTESAFHYEADGANVLAGIMNRNDEKCHLDASGAYTQYKTQDYPSVPTLVRLLAKHNIIPIFAVTNYSYSYYEKLHKYFPVSSLGVLQEDSSNIVELLEEAFYRIRSNLDIRALDSPRGLRTEVTSDTLQKTETGSFHIKRGEVGTYNVHLRAVEDIDGTHVCQLAKEDQGGNIHLKPSFSDGLRMDASVICDVCPCELQKEVRSARCHFRGDFMCGHCVCNEGWSGKTCNCSTGSLSDTQPCLREGEDKPCSGHGECQCGRCVCYGEGRYEGHFCEYDNFQCPRTSGFLCNDRGRCSMGECVCEPGWTGRSCDCPLSNATCIDSNGGICNGRGYCECGRCHCNQQSLYTDTTCEINYSAIRLGLCEDLRSCVQCQAWGTGEKKGRACDDCPFKVKMVDELKKAEEVVEYCSFRDEDDDCTYSYNVEGDGSPGPNSTVLVHKKKDCPPGSFWWLIPLLIFLLLLLALLLLLCWKYCACCKACLGLLPCCNRGHMVGFKEDHYMLRENLMASDHLDTPMLRSGNLKGRDTVRWKITNNVQRPGFATHAASTSPTELVPYGLSLRLGRLCTENLMKPGTRECDQLRQEVEENLNEVYRQVSGAHKLQQTKFRQQPNTGKKQDHTIVDTVLLAPRSAKQMLLKLTEKQVEQGSFHELKVAPGYYTVTAEQDARGMVEFQEGVELVDVRVPLFIRPEDDDEKQLLVEAIDVPVGTATLGRRLVNITIIKEQASGVVSFEQPEYSVSRGDQVARIPVIRHILDNGKSQVSYSTQDNTAHGHRDYVPVEGELLFHPGETWKELQVKLLELQEVDSLLRGRQVRRFQVQLSNPKFGARLGQPSTTTVILGEHDETDRSLINQTLSSPPPPHGDLGAPQNPNAKAAGSRKIHFNWLPPPGKPMGYRVKYWIQGDSESEAHLLDSKVPSVELTNLYPYCDYEMKVCAYGAQGEGPYSSLVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYGLVNEDNRPIGPMKKVLVDNPKNRMLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDIPIVDAQGGEDYENFLMYSDDVLRSPASSQRPSVSDDTGCGWKFEPLLGEELDLRRVTWRLPPELIPRLSASSGRSDEDGSVAGGVEGEGSGWIRGATPRPPGEHLVNGRMDFAYPGSANSLHRMTAANVAYGTHLSPHLSHRVLSTSSTLTRDYHSLTRTEHSHSGTLPRDYSTLTSLSSQGLPPIWEDGRSRLPLSWTLGSLSRAHMKGVPASRGSPDSIILAGQSAAPSWGTDSRGAVGVPDTPTRLVFSALGPTSLKVSWQEPQCDRMLLGYSVEYQLLNGGEMHRLNIPNPGQTSVVVEDLLPNHSYVFRVRAQSQEGWGREREGVITIESQVHPQSPLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPNGDILGYLVTCEMAQGGAPARTFRVDGDNPESRLTVPGLSENVPYKFKVQARTTEGFGPEREGIITIESQVGGPFPQLGSHSGLFQNPVQSEFSSVTSTHSTTTEPFLMDGLTLGTQRLEAGGSLTRHVTQEFVTRTLTASGSLSTHMDQQFFQT

Target ORF information:

RefSeq Version	XM_017314349.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus integrin beta 4 (Itgb4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314349.2

ORF Insert Sequence:

ATGGCAGGGC CCTGTTGCAG CCCATGGGTG AAGCTGCTGC TGCTGGCAGC AATGCTGAGT 
GCCAGCCTCC CTGGAGACCT GGCCAACCGC TGCAAGAAGG CTCAGGTGAA GAGCTGTACC 
GAGTGCATCC GGGTGGACAA GAGCTGTGCC TACTGCACAG ACGAGCTGTT CAAGGAGAGG 
CGCTGCAACA CCCAGGCGGA GCTTCTGGCT GCAGGCTGCA GGGGAGAGAG CATCCTGGTC 
ATGGAGAGCA GCCTTGAAAT CACAGAGAAC ACCCAGATCG ACACCAGCCT GCACCGCAGC 
CAGGTATCTC CCCAAGGCCT GCAAGTCCGG CTGCGGCCGG GTGAGGAGCG CAGCTTTGTG 
TTCCAGGTGT TTGAGCCCCT GGAGAGCCCC GTGGATCTGT ATATCCTCAT GGACTTCTCC 
AACTCCATGT CTGACGATCT GGACAACCTC AAGCAGATGG GGCAGAACCT GGCCAAGATC 
CTGCGCCAGC TCACCAGCGA CTACACCATT GGATTTGGAA AGTTTGTGGA CAAAGTCAGC 
GTCCCACAGA CAGACATGAG GCCCGAGAAA CTGAAGGAGC CCTGGCCCAA CAGTGATCCC 
CCGTTCTCCT TCAAGAACGT TATCAGCTTA ACGGAGAATG TGGAAGAATT CTGGAACAAA 
CTGCAAGGAG AACGCATCTC AGGCAACCTG GACGCTCCTG AAGGGGGCTT TGATGCCATC 
CTGCAGACAG CTGTGTGCAC AAGGGACATT GGCTGGAGGG CTGACAGCAC CCACCTGCTG 
GTGTTCTCCA CCGAGTCTGC CTTCCACTAC GAGGCTGATG GTGCCAACGT GCTGGCCGGC 
ATCATGAACC GCAATGATGA GAAATGCCAC CTGGACGCCT CGGGCGCCTA CACCCAATAC 
AAGACACAGG ACTACCCATC AGTGCCCACG CTGGTTCGCC TGCTTGCCAA GCATAACATC 
ATCCCCATCT TTGCTGTCAC CAACTACTCT TACAGCTACT ATGAGAAGCT CCATAAGTAT 
TTCCCCGTCT CCTCTCTGGG CGTCCTGCAG GAGGATTCAT CCAACATCGT GGAGCTGCTG 
GAGGAGGCCT TCTATCGAAT TCGCTCCAAC CTGGACATCC GGGCTCTGGA CAGCCCCAGA 
GGCCTGAGAA CAGAGGTCAC CTCCGATACT CTCCAGAAGA CGGAGACTGG GTCCTTTCAC 
ATCAAGCGGG GGGAAGTGGG CACATACAAT GTGCATCTCC GGGCAGTGGA GGACATAGAT 
GGGACACATG TGTGCCAGCT GGCTAAAGAA GACCAAGGGG GCAACATCCA CCTGAAACCC 
TCCTTCTCTG ATGGCCTCCG GATGGACGCG AGTGTGATCT GTGACGTGTG CCCCTGTGAG 
CTGCAAAAGG AAGTTCGATC AGCTCGCTGT CACTTCAGAG GAGACTTCAT GTGTGGACAC 
TGTGTGTGCA ATGAGGGCTG GAGTGGCAAA ACCTGCAACT GCTCCACCGG CTCTCTGAGT 
GACACACAGC CCTGCCTGCG TGAGGGTGAG GACAAACCGT GCTCGGGCCA CGGCGAGTGC 
CAGTGCGGAC GCTGTGTGTG CTATGGTGAA GGCCGCTACG AGGGTCACTT CTGCGAGTAT 
GACAACTTCC AGTGTCCCCG GACCTCTGGA TTCCTGTGCA ATGACCGGGG TCGCTGTTCT 
ATGGGAGAGT GTGTGTGTGA GCCTGGTTGG ACAGGCCGCA GCTGTGACTG TCCCCTCAGC 
AATGCCACCT GCATCGATAG CAACGGGGGC ATCTGCAACG GCCGAGGCTA CTGTGAGTGT 
GGCCGTTGTC ACTGCAACCA GCAGTCGCTC TACACGGACA CCACCTGTGA GATCAACTAC 
TCTGCGATAC GCCTGGGTCT CTGTGAGGAT CTCCGCTCCT GCGTACAGTG CCAGGCCTGG 
GGCACCGGGG AGAAGAAAGG GCGCGCGTGT GACGATTGCC CCTTTAAAGT CAAGATGGTA 
GACGAGCTTA AGAAAGCAGA AGAGGTGGTG GAGTACTGCT CCTTCCGGGA TGAGGATGAC 
GACTGCACTT ACAGCTACAA CGTGGAGGGC GATGGCAGCC CTGGGCCCAA CAGCACAGTC 
CTGGTCCACA AAAAGAAAGA CTGCCCTCCC GGCTCCTTCT GGTGGCTCAT CCCCCTGCTC 
ATCTTCCTCC TGTTGCTCCT GGCGTTGCTT CTGCTGCTCT GCTGGAAATA CTGTGCCTGC 
TGCAAAGCCT GCCTGGGGCT TCTTCCTTGC TGCAACCGAG GTCACATGGT GGGCTTTAAG 
GAAGATCACT ATATGCTTCG GGAGAACCTG ATGGCCTCTG ACCACCTGGA CACGCCCATG 
CTACGAAGCG GGAACCTCAA GGGACGAGAC ACAGTCCGCT GGAAGATCAC CAACAATGTG 
CAGCGCCCTG GCTTTGCCAC CCATGCCGCC AGCACCAGCC CCACGGAGCT CGTACCCTAC 
GGGCTGTCCC TGCGCCTTGG CCGCCTCTGC ACTGAGAACC TTATGAAGCC GGGCACCCGA 
GAGTGTGACC AGCTACGCCA GGAGGTGGAG GAAAATCTGA ATGAGGTGTA TAGACAGGTC 
AGCGGCGCAC ACAAGCTCCA GCAGACGAAG TTCCGACAGC AGCCCAACAC CGGGAAAAAG 
CAAGACCACA CCATTGTGGA CACAGTGTTG CTGGCGCCCC GCTCCGCCAA GCAGATGCTG 
CTGAAGCTGA CAGAGAAGCA GGTGGAGCAG GGGTCCTTCC ATGAACTGAA GGTGGCCCCT 
GGCTACTACA CTGTCACGGC AGAGCAGGAT GCCCGGGGCA TGGTGGAGTT CCAGGAGGGC 
GTGGAGCTGG TGGATGTGCG AGTGCCCCTC TTCATCCGGC CTGAGGATGA TGATGAGAAG 
CAGCTGCTGG TGGAGGCCAT TGATGTCCCT GTGGGCACTG CCACCCTTGG TCGCCGTCTG 
GTAAACATCA CCATTATCAA GGAACAAGCT AGTGGGGTAG TGTCCTTCGA GCAGCCTGAA 
TACTCGGTGA GTCGTGGAGA CCAGGTGGCC CGCATCCCTG TCATCCGGCA CATCCTGGAC 
AATGGCAAGT CCCAGGTCTC CTATAGCACA CAGGATAATA CAGCACACGG ACACCGGGAT 
TATGTCCCTG TGGAGGGAGA GCTGCTGTTC CATCCTGGGG AGACCTGGAA GGAGTTGCAG 
GTGAAGCTAC TGGAGCTGCA GGAGGTCGAC TCCCTCCTGC GTGGCCGCCA GGTCCGCCGC 
TTCCAAGTCC AACTCAGCAA CCCCAAGTTC GGAGCCCGCC TGGGCCAGCC CAGCACAACC 
ACCGTTATTC TCGGTGAGCA CGATGAAACG GACAGGAGTC TCATAAATCA AACACTTTCA 
TCGCCTCCGC CACCCCATGG AGACCTGGGC GCCCCACAGA ACCCCAATGC CAAGGCTGCC 
GGGTCCAGGA AGATCCATTT CAACTGGCTG CCCCCTCCTG GCAAGCCAAT GGGGTACAGG 
GTGAAGTACT GGATCCAGGG CGACTCTGAG TCTGAAGCCC ACCTTCTAGA CAGCAAGGTG 
CCCTCAGTGG AACTCACCAA CCTGTATCCC TATTGCGACT ACGAAATGAA GGTGTGTGCC 
TATGGGGCAC AGGGTGAGGG GCCCTATAGC TCACTGGTGT CCTGCCGCAC CCACCAGGAA 
GTACCCAGCG AGCCAGGGAG GCTGGCTTTC AATGTAGTCT CTTCTACGGT GACGCAGCTG 
AGCTGGGCAG AGCCAGCTGA GACCAATGGC GAGATCACAG CCTACGAGGT CTGCTATGGA 
CTGGTCAATG AGGACAACCG ACCCATTGGA CCTATGAAGA AGGTGCTCGT GGACAACCCC 
AAGAACCGGA TGCTGCTCAT TGAGAATCTG CGAGAATCCC AGCCATACCG ATACACGGTT 
AAGGCGCGCA ATGGGGCAGG ATGGGGACCC GAGAGAGAGG CTATCATCAA CCTGGCTACA 
CAGCCCAAGC GGCCCATGTC CATCCCTATC ATCCCAGACA TCCCCATAGT GGACGCCCAG 
GGTGGAGAAG ACTACGAAAA CTTCCTTATG TACAGTGATG ACGTCCTGCG GTCCCCAGCC 
AGCAGCCAGA GGCCCAGCGT TTCTGATGAC ACTGGCTGCG GCTGGAAGTT CGAGCCTCTG 
CTCGGGGAGG AGCTGGACCT GCGGCGCGTC ACGTGGCGGC TACCCCCGGA ACTCATCCCG 
CGCCTGTCCG CCAGCAGCGG GCGCTCTGAC GAAGACGGCA GCGTTGCGGG GGGCGTGGAA 
GGGGAGGGCA GCGGCTGGAT CCGCGGGGCT ACGCCCCGAC CCCCGGGAGA GCACCTGGTG 
AATGGCCGGA TGGACTTTGC CTATCCAGGC AGCGCCAACT CCCTGCACAG AATGACTGCA 
GCCAATGTGG CCTATGGCAC GCATCTGAGC CCACACCTGT CCCACCGAGT GCTGAGCACG 
TCCTCCACCC TTACCCGGGA CTACCACTCT CTGACACGCA CAGAGCACTC CCACTCAGGC 
ACACTTCCCA GGGACTACTC CACCCTCACT TCCCTTTCCT CCCAAGGCCT CCCTCCTATC 
TGGGAAGATG GGAGGAGCAG GCTTCCGCTG TCCTGGACTC TTGGGTCCTT GAGCCGGGCT 
CACATGAAGG GTGTGCCCGC ATCCAGGGGT TCACCAGACT CTATAATCCT GGCCGGGCAG 
TCAGCAGCAC CCTCCTGGGG TACAGATTCC CGTGGGGCTG TGGGTGTGCC TGACACACCC 
ACTCGGCTGG TGTTCTCTGC CCTGGGGCCC ACGTCTTTGA AGGTGAGCTG GCAGGAGCCA 
CAGTGTGATC GGATGCTGCT GGGCTACAGT GTGGAATACC AGCTACTAAA CGGCGGCGAG 
ATGCATCGGC TCAACATCCC TAACCCTGGC CAAACCTCGG TGGTGGTAGA GGATCTCCTG 
CCTAACCACT CCTATGTGTT CCGGGTACGG GCCCAGAGCC AGGAGGGCTG GGGCCGAGAG 
CGAGAGGGTG TCATCACCAT CGAGTCCCAG GTGCACCCGC AGAGCCCTCT CTGCCCCCTG 
CCAGGCTCAG CCTTCACTCT GAGCACCCCC AGCGCCCCGG GACCGCTGGT GTTCACTGCC 
CTAAGCCCAG ACTCCCTGCA GCTCAGCTGG GAGCGGCCGA GGAGGCCCAA TGGAGATATC 
CTTGGCTACC TGGTGACCTG TGAGATGGCC CAAGGAGGAG CACCAGCCAG GACCTTCCGG 
GTGGACGGAG ACAACCCTGA GAGCCGGTTG ACTGTACCTG GCCTCAGTGA GAACGTTCCT 
TACAAGTTCA AGGTTCAGGC CAGGACGACC GAGGGCTTCG GGCCAGAGCG TGAGGGTATC 
ATCACCATCG AGTCTCAGGT TGGAGGCCCC TTCCCACAGC TGGGCAGCCA TTCTGGGCTC 
TTCCAGAACC CAGTGCAAAG CGAGTTCAGC AGCGTGACCA GCACGCACAG CACCACGACT 
GAGCCCTTCC TCATGGATGG TCTAACCCTG GGGACCCAGC GCCTGGAAGC AGGAGGCTCC 
CTCACCCGGC ATGTGACCCA GGAATTCGTG ACCCGGACCT TAACGGCCAG TGGCTCTCTC 
AGCACTCATA TGGACCAACA GTTCTTCCAA ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itgb4 ( XM_017314349.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169838.1 Mus musculus integrin beta 4 (Itgb4), transcript variant X1, mRNA.

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CloneID	OMu77781
Clone ID Related Accession (Same CDS sequence)	XM_017314349.2 , XM_017314349.1
Accession Version	XM_017314349.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	integrin beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGC CCTGTTGCAG CCCATGGGTG AAGCTGCTGC TGCTGGCAGC AATGCTGAGT 
GCCAGCCTCC CTGGAGACCT GGCCAACCGC TGCAAGAAGG CTCAGGTGAA GAGCTGTACC 
GAGTGCATCC GGGTGGACAA GAGCTGTGCC TACTGCACAG ACGAGCTGTT CAAGGAGAGG 
CGCTGCAACA CCCAGGCGGA GCTTCTGGCT GCAGGCTGCA GGGGAGAGAG CATCCTGGTC 
ATGGAGAGCA GCCTTGAAAT CACAGAGAAC ACCCAGATCG ACACCAGCCT GCACCGCAGC 
CAGGTATCTC CCCAAGGCCT GCAAGTCCGG CTGCGGCCGG GTGAGGAGCG CAGCTTTGTG 
TTCCAGGTGT TTGAGCCCCT GGAGAGCCCC GTGGATCTGT ATATCCTCAT GGACTTCTCC 
AACTCCATGT CTGACGATCT GGACAACCTC AAGCAGATGG GGCAGAACCT GGCCAAGATC 
CTGCGCCAGC TCACCAGCGA CTACACCATT GGATTTGGAA AGTTTGTGGA CAAAGTCAGC 
GTCCCACAGA CAGACATGAG GCCCGAGAAA CTGAAGGAGC CCTGGCCCAA CAGTGATCCC 
CCGTTCTCCT TCAAGAACGT TATCAGCTTA ACGGAGAATG TGGAAGAATT CTGGAACAAA 
CTGCAAGGAG AACGCATCTC AGGCAACCTG GACGCTCCTG AAGGGGGCTT TGATGCCATC 
CTGCAGACAG CTGTGTGCAC AAGGGACATT GGCTGGAGGG CTGACAGCAC CCACCTGCTG 
GTGTTCTCCA CCGAGTCTGC CTTCCACTAC GAGGCTGATG GTGCCAACGT GCTGGCCGGC 
ATCATGAACC GCAATGATGA GAAATGCCAC CTGGACGCCT CGGGCGCCTA CACCCAATAC 
AAGACACAGG ACTACCCATC AGTGCCCACG CTGGTTCGCC TGCTTGCCAA GCATAACATC 
ATCCCCATCT TTGCTGTCAC CAACTACTCT TACAGCTACT ATGAGAAGCT CCATAAGTAT 
TTCCCCGTCT CCTCTCTGGG CGTCCTGCAG GAGGATTCAT CCAACATCGT GGAGCTGCTG 
GAGGAGGCCT TCTATCGAAT TCGCTCCAAC CTGGACATCC GGGCTCTGGA CAGCCCCAGA 
GGCCTGAGAA CAGAGGTCAC CTCCGATACT CTCCAGAAGA CGGAGACTGG GTCCTTTCAC 
ATCAAGCGGG GGGAAGTGGG CACATACAAT GTGCATCTCC GGGCAGTGGA GGACATAGAT 
GGGACACATG TGTGCCAGCT GGCTAAAGAA GACCAAGGGG GCAACATCCA CCTGAAACCC 
TCCTTCTCTG ATGGCCTCCG GATGGACGCG AGTGTGATCT GTGACGTGTG CCCCTGTGAG 
CTGCAAAAGG AAGTTCGATC AGCTCGCTGT CACTTCAGAG GAGACTTCAT GTGTGGACAC 
TGTGTGTGCA ATGAGGGCTG GAGTGGCAAA ACCTGCAACT GCTCCACCGG CTCTCTGAGT 
GACACACAGC CCTGCCTGCG TGAGGGTGAG GACAAACCGT GCTCGGGCCA CGGCGAGTGC 
CAGTGCGGAC GCTGTGTGTG CTATGGTGAA GGCCGCTACG AGGGTCACTT CTGCGAGTAT 
GACAACTTCC AGTGTCCCCG GACCTCTGGA TTCCTGTGCA ATGACCGGGG TCGCTGTTCT 
ATGGGAGAGT GTGTGTGTGA GCCTGGTTGG ACAGGCCGCA GCTGTGACTG TCCCCTCAGC 
AATGCCACCT GCATCGATAG CAACGGGGGC ATCTGCAACG GCCGAGGCTA CTGTGAGTGT 
GGCCGTTGTC ACTGCAACCA GCAGTCGCTC TACACGGACA CCACCTGTGA GATCAACTAC 
TCTGCGATAC GCCTGGGTCT CTGTGAGGAT CTCCGCTCCT GCGTACAGTG CCAGGCCTGG 
GGCACCGGGG AGAAGAAAGG GCGCGCGTGT GACGATTGCC CCTTTAAAGT CAAGATGGTA 
GACGAGCTTA AGAAAGCAGA AGAGGTGGTG GAGTACTGCT CCTTCCGGGA TGAGGATGAC 
GACTGCACTT ACAGCTACAA CGTGGAGGGC GATGGCAGCC CTGGGCCCAA CAGCACAGTC 
CTGGTCCACA AAAAGAAAGA CTGCCCTCCC GGCTCCTTCT GGTGGCTCAT CCCCCTGCTC 
ATCTTCCTCC TGTTGCTCCT GGCGTTGCTT CTGCTGCTCT GCTGGAAATA CTGTGCCTGC 
TGCAAAGCCT GCCTGGGGCT TCTTCCTTGC TGCAACCGAG GTCACATGGT GGGCTTTAAG 
GAAGATCACT ATATGCTTCG GGAGAACCTG ATGGCCTCTG ACCACCTGGA CACGCCCATG 
CTACGAAGCG GGAACCTCAA GGGACGAGAC ACAGTCCGCT GGAAGATCAC CAACAATGTG 
CAGCGCCCTG GCTTTGCCAC CCATGCCGCC AGCACCAGCC CCACGGAGCT CGTACCCTAC 
GGGCTGTCCC TGCGCCTTGG CCGCCTCTGC ACTGAGAACC TTATGAAGCC GGGCACCCGA 
GAGTGTGACC AGCTACGCCA GGAGGTGGAG GAAAATCTGA ATGAGGTGTA TAGACAGGTC 
AGCGGCGCAC ACAAGCTCCA GCAGACGAAG TTCCGACAGC AGCCCAACAC CGGGAAAAAG 
CAAGACCACA CCATTGTGGA CACAGTGTTG CTGGCGCCCC GCTCCGCCAA GCAGATGCTG 
CTGAAGCTGA CAGAGAAGCA GGTGGAGCAG GGGTCCTTCC ATGAACTGAA GGTGGCCCCT 
GGCTACTACA CTGTCACGGC AGAGCAGGAT GCCCGGGGCA TGGTGGAGTT CCAGGAGGGC 
GTGGAGCTGG TGGATGTGCG AGTGCCCCTC TTCATCCGGC CTGAGGATGA TGATGAGAAG 
CAGCTGCTGG TGGAGGCCAT TGATGTCCCT GTGGGCACTG CCACCCTTGG TCGCCGTCTG 
GTAAACATCA CCATTATCAA GGAACAAGCT AGTGGGGTAG TGTCCTTCGA GCAGCCTGAA 
TACTCGGTGA GTCGTGGAGA CCAGGTGGCC CGCATCCCTG TCATCCGGCA CATCCTGGAC 
AATGGCAAGT CCCAGGTCTC CTATAGCACA CAGGATAATA CAGCACACGG ACACCGGGAT 
TATGTCCCTG TGGAGGGAGA GCTGCTGTTC CATCCTGGGG AGACCTGGAA GGAGTTGCAG 
GTGAAGCTAC TGGAGCTGCA GGAGGTCGAC TCCCTCCTGC GTGGCCGCCA GGTCCGCCGC 
TTCCAAGTCC AACTCAGCAA CCCCAAGTTC GGAGCCCGCC TGGGCCAGCC CAGCACAACC 
ACCGTTATTC TCGGTGAGCA CGATGAAACG GACAGGAGTC TCATAAATCA AACACTTTCA 
TCGCCTCCGC CACCCCATGG AGACCTGGGC GCCCCACAGA ACCCCAATGC CAAGGCTGCC 
GGGTCCAGGA AGATCCATTT CAACTGGCTG CCCCCTCCTG GCAAGCCAAT GGGGTACAGG 
GTGAAGTACT GGATCCAGGG CGACTCTGAG TCTGAAGCCC ACCTTCTAGA CAGCAAGGTG 
CCCTCAGTGG AACTCACCAA CCTGTATCCC TATTGCGACT ACGAAATGAA GGTGTGTGCC 
TATGGGGCAC AGGGTGAGGG GCCCTATAGC TCACTGGTGT CCTGCCGCAC CCACCAGGAA 
GTACCCAGCG AGCCAGGGAG GCTGGCTTTC AATGTAGTCT CTTCTACGGT GACGCAGCTG 
AGCTGGGCAG AGCCAGCTGA GACCAATGGC GAGATCACAG CCTACGAGGT CTGCTATGGA 
CTGGTCAATG AGGACAACCG ACCCATTGGA CCTATGAAGA AGGTGCTCGT GGACAACCCC 
AAGAACCGGA TGCTGCTCAT TGAGAATCTG CGAGAATCCC AGCCATACCG ATACACGGTT 
AAGGCGCGCA ATGGGGCAGG ATGGGGACCC GAGAGAGAGG CTATCATCAA CCTGGCTACA 
CAGCCCAAGC GGCCCATGTC CATCCCTATC ATCCCAGACA TCCCCATAGT GGACGCCCAG 
GGTGGAGAAG ACTACGAAAA CTTCCTTATG TACAGTGATG ACGTCCTGCG GTCCCCAGCC 
AGCAGCCAGA GGCCCAGCGT TTCTGATGAC ACTGGCTGCG GCTGGAAGTT CGAGCCTCTG 
CTCGGGGAGG AGCTGGACCT GCGGCGCGTC ACGTGGCGGC TACCCCCGGA ACTCATCCCG 
CGCCTGTCCG CCAGCAGCGG GCGCTCTGAC GAAGACGGCA GCGTTGCGGG GGGCGTGGAA 
GGGGAGGGCA GCGGCTGGAT CCGCGGGGCT ACGCCCCGAC CCCCGGGAGA GCACCTGGTG 
AATGGCCGGA TGGACTTTGC CTATCCAGGC AGCGCCAACT CCCTGCACAG AATGACTGCA 
GCCAATGTGG CCTATGGCAC GCATCTGAGC CCACACCTGT CCCACCGAGT GCTGAGCACG 
TCCTCCACCC TTACCCGGGA CTACCACTCT CTGACACGCA CAGAGCACTC CCACTCAGGC 
ACACTTCCCA GGGACTACTC CACCCTCACT TCCCTTTCCT CCCAAGGCCT CCCTCCTATC 
TGGGAAGATG GGAGGAGCAG GCTTCCGCTG TCCTGGACTC TTGGGTCCTT GAGCCGGGCT 
CACATGAAGG GTGTGCCCGC ATCCAGGGGT TCACCAGACT CTATAATCCT GGCCGGGCAG 
TCAGCAGCAC CCTCCTGGGG TACAGATTCC CGTGGGGCTG TGGGTGTGCC TGACACACCC 
ACTCGGCTGG TGTTCTCTGC CCTGGGGCCC ACGTCTTTGA AGGTGAGCTG GCAGGAGCCA 
CAGTGTGATC GGATGCTGCT GGGCTACAGT GTGGAATACC AGCTACTAAA CGGCGGCGAG 
ATGCATCGGC TCAACATCCC TAACCCTGGC CAAACCTCGG TGGTGGTAGA GGATCTCCTG 
CCTAACCACT CCTATGTGTT CCGGGTACGG GCCCAGAGCC AGGAGGGCTG GGGCCGAGAG 
CGAGAGGGTG TCATCACCAT CGAGTCCCAG GTGCACCCGC AGAGCCCTCT CTGCCCCCTG 
CCAGGCTCAG CCTTCACTCT GAGCACCCCC AGCGCCCCGG GACCGCTGGT GTTCACTGCC 
CTAAGCCCAG ACTCCCTGCA GCTCAGCTGG GAGCGGCCGA GGAGGCCCAA TGGAGATATC 
CTTGGCTACC TGGTGACCTG TGAGATGGCC CAAGGAGGAG CACCAGCCAG GACCTTCCGG 
GTGGACGGAG ACAACCCTGA GAGCCGGTTG ACTGTACCTG GCCTCAGTGA GAACGTTCCT 
TACAAGTTCA AGGTTCAGGC CAGGACGACC GAGGGCTTCG GGCCAGAGCG TGAGGGTATC 
ATCACCATCG AGTCTCAGGT TGGAGGCCCC TTCCCACAGC TGGGCAGCCA TTCTGGGCTC 
TTCCAGAACC CAGTGCAAAG CGAGTTCAGC AGCGTGACCA GCACGCACAG CACCACGACT 
GAGCCCTTCC TCATGGATGG TCTAACCCTG GGGACCCAGC GCCTGGAAGC AGGAGGCTCC 
CTCACCCGGC ATGTGACCCA GGAATTCGTG ACCCGGACCT TAACGGCCAG TGGCTCTCTC 
AGCACTCATA TGGACCAACA GTTCTTCCAA ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169838.1
CDS	436..6051
Translation	MAGPCCSPWVKLLLLAAMLSASLPGDLANRCKKAQVKSCTECIRVDKSCAYCTDELFKERRCNTQAELLAAGCRGESILVMESSLEITENTQIDTSLHRSQVSPQGLQVRLRPGEERSFVFQVFEPLESPVDLYILMDFSNSMSDDLDNLKQMGQNLAKILRQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTENVEEFWNKLQGERISGNLDAPEGGFDAILQTAVCTRDIGWRADSTHLLVFSTESAFHYEADGANVLAGIMNRNDEKCHLDASGAYTQYKTQDYPSVPTLVRLLAKHNIIPIFAVTNYSYSYYEKLHKYFPVSSLGVLQEDSSNIVELLEEAFYRIRSNLDIRALDSPRGLRTEVTSDTLQKTETGSFHIKRGEVGTYNVHLRAVEDIDGTHVCQLAKEDQGGNIHLKPSFSDGLRMDASVICDVCPCELQKEVRSARCHFRGDFMCGHCVCNEGWSGKTCNCSTGSLSDTQPCLREGEDKPCSGHGECQCGRCVCYGEGRYEGHFCEYDNFQCPRTSGFLCNDRGRCSMGECVCEPGWTGRSCDCPLSNATCIDSNGGICNGRGYCECGRCHCNQQSLYTDTTCEINYSAIRLGLCEDLRSCVQCQAWGTGEKKGRACDDCPFKVKMVDELKKAEEVVEYCSFRDEDDDCTYSYNVEGDGSPGPNSTVLVHKKKDCPPGSFWWLIPLLIFLLLLLALLLLLCWKYCACCKACLGLLPCCNRGHMVGFKEDHYMLRENLMASDHLDTPMLRSGNLKGRDTVRWKITNNVQRPGFATHAASTSPTELVPYGLSLRLGRLCTENLMKPGTRECDQLRQEVEENLNEVYRQVSGAHKLQQTKFRQQPNTGKKQDHTIVDTVLLAPRSAKQMLLKLTEKQVEQGSFHELKVAPGYYTVTAEQDARGMVEFQEGVELVDVRVPLFIRPEDDDEKQLLVEAIDVPVGTATLGRRLVNITIIKEQASGVVSFEQPEYSVSRGDQVARIPVIRHILDNGKSQVSYSTQDNTAHGHRDYVPVEGELLFHPGETWKELQVKLLELQEVDSLLRGRQVRRFQVQLSNPKFGARLGQPSTTTVILGEHDETDRSLINQTLSSPPPPHGDLGAPQNPNAKAAGSRKIHFNWLPPPGKPMGYRVKYWIQGDSESEAHLLDSKVPSVELTNLYPYCDYEMKVCAYGAQGEGPYSSLVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYGLVNEDNRPIGPMKKVLVDNPKNRMLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDIPIVDAQGGEDYENFLMYSDDVLRSPASSQRPSVSDDTGCGWKFEPLLGEELDLRRVTWRLPPELIPRLSASSGRSDEDGSVAGGVEGEGSGWIRGATPRPPGEHLVNGRMDFAYPGSANSLHRMTAANVAYGTHLSPHLSHRVLSTSSTLTRDYHSLTRTEHSHSGTLPRDYSTLTSLSSQGLPPIWEDGRSRLPLSWTLGSLSRAHMKGVPASRGSPDSIILAGQSAAPSWGTDSRGAVGVPDTPTRLVFSALGPTSLKVSWQEPQCDRMLLGYSVEYQLLNGGEMHRLNIPNPGQTSVVVEDLLPNHSYVFRVRAQSQEGWGREREGVITIESQVHPQSPLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPNGDILGYLVTCEMAQGGAPARTFRVDGDNPESRLTVPGLSENVPYKFKVQARTTEGFGPEREGIITIESQVGGPFPQLGSHSGLFQNPVQSEFSSVTSTHSTTTEPFLMDGLTLGTQRLEAGGSLTRHVTQEFVTRTLTASGSLSTHMDQQFFQT

Target ORF information:

RefSeq Version	XM_017314349.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus integrin beta 4 (Itgb4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314349.1

ORF Insert Sequence:

ATGGCAGGGC CCTGTTGCAG CCCATGGGTG AAGCTGCTGC TGCTGGCAGC AATGCTGAGT 
GCCAGCCTCC CTGGAGACCT GGCCAACCGC TGCAAGAAGG CTCAGGTGAA GAGCTGTACC 
GAGTGCATCC GGGTGGACAA GAGCTGTGCC TACTGCACAG ACGAGCTGTT CAAGGAGAGG 
CGCTGCAACA CCCAGGCGGA GCTTCTGGCT GCAGGCTGCA GGGGAGAGAG CATCCTGGTC 
ATGGAGAGCA GCCTTGAAAT CACAGAGAAC ACCCAGATCG ACACCAGCCT GCACCGCAGC 
CAGGTATCTC CCCAAGGCCT GCAAGTCCGG CTGCGGCCGG GTGAGGAGCG CAGCTTTGTG 
TTCCAGGTGT TTGAGCCCCT GGAGAGCCCC GTGGATCTGT ATATCCTCAT GGACTTCTCC 
AACTCCATGT CTGACGATCT GGACAACCTC AAGCAGATGG GGCAGAACCT GGCCAAGATC 
CTGCGCCAGC TCACCAGCGA CTACACCATT GGATTTGGAA AGTTTGTGGA CAAAGTCAGC 
GTCCCACAGA CAGACATGAG GCCCGAGAAA CTGAAGGAGC CCTGGCCCAA CAGTGATCCC 
CCGTTCTCCT TCAAGAACGT TATCAGCTTA ACGGAGAATG TGGAAGAATT CTGGAACAAA 
CTGCAAGGAG AACGCATCTC AGGCAACCTG GACGCTCCTG AAGGGGGCTT TGATGCCATC 
CTGCAGACAG CTGTGTGCAC AAGGGACATT GGCTGGAGGG CTGACAGCAC CCACCTGCTG 
GTGTTCTCCA CCGAGTCTGC CTTCCACTAC GAGGCTGATG GTGCCAACGT GCTGGCCGGC 
ATCATGAACC GCAATGATGA GAAATGCCAC CTGGACGCCT CGGGCGCCTA CACCCAATAC 
AAGACACAGG ACTACCCATC AGTGCCCACG CTGGTTCGCC TGCTTGCCAA GCATAACATC 
ATCCCCATCT TTGCTGTCAC CAACTACTCT TACAGCTACT ATGAGAAGCT CCATAAGTAT 
TTCCCCGTCT CCTCTCTGGG CGTCCTGCAG GAGGATTCAT CCAACATCGT GGAGCTGCTG 
GAGGAGGCCT TCTATCGAAT TCGCTCCAAC CTGGACATCC GGGCTCTGGA CAGCCCCAGA 
GGCCTGAGAA CAGAGGTCAC CTCCGATACT CTCCAGAAGA CGGAGACTGG GTCCTTTCAC 
ATCAAGCGGG GGGAAGTGGG CACATACAAT GTGCATCTCC GGGCAGTGGA GGACATAGAT 
GGGACACATG TGTGCCAGCT GGCTAAAGAA GACCAAGGGG GCAACATCCA CCTGAAACCC 
TCCTTCTCTG ATGGCCTCCG GATGGACGCG AGTGTGATCT GTGACGTGTG CCCCTGTGAG 
CTGCAAAAGG AAGTTCGATC AGCTCGCTGT CACTTCAGAG GAGACTTCAT GTGTGGACAC 
TGTGTGTGCA ATGAGGGCTG GAGTGGCAAA ACCTGCAACT GCTCCACCGG CTCTCTGAGT 
GACACACAGC CCTGCCTGCG TGAGGGTGAG GACAAACCGT GCTCGGGCCA CGGCGAGTGC 
CAGTGCGGAC GCTGTGTGTG CTATGGTGAA GGCCGCTACG AGGGTCACTT CTGCGAGTAT 
GACAACTTCC AGTGTCCCCG GACCTCTGGA TTCCTGTGCA ATGACCGGGG TCGCTGTTCT 
ATGGGAGAGT GTGTGTGTGA GCCTGGTTGG ACAGGCCGCA GCTGTGACTG TCCCCTCAGC 
AATGCCACCT GCATCGATAG CAACGGGGGC ATCTGCAACG GCCGAGGCTA CTGTGAGTGT 
GGCCGTTGTC ACTGCAACCA GCAGTCGCTC TACACGGACA CCACCTGTGA GATCAACTAC 
TCTGCGATAC GCCTGGGTCT CTGTGAGGAT CTCCGCTCCT GCGTACAGTG CCAGGCCTGG 
GGCACCGGGG AGAAGAAAGG GCGCGCGTGT GACGATTGCC CCTTTAAAGT CAAGATGGTA 
GACGAGCTTA AGAAAGCAGA AGAGGTGGTG GAGTACTGCT CCTTCCGGGA TGAGGATGAC 
GACTGCACTT ACAGCTACAA CGTGGAGGGC GATGGCAGCC CTGGGCCCAA CAGCACAGTC 
CTGGTCCACA AAAAGAAAGA CTGCCCTCCC GGCTCCTTCT GGTGGCTCAT CCCCCTGCTC 
ATCTTCCTCC TGTTGCTCCT GGCGTTGCTT CTGCTGCTCT GCTGGAAATA CTGTGCCTGC 
TGCAAAGCCT GCCTGGGGCT TCTTCCTTGC TGCAACCGAG GTCACATGGT GGGCTTTAAG 
GAAGATCACT ATATGCTTCG GGAGAACCTG ATGGCCTCTG ACCACCTGGA CACGCCCATG 
CTACGAAGCG GGAACCTCAA GGGACGAGAC ACAGTCCGCT GGAAGATCAC CAACAATGTG 
CAGCGCCCTG GCTTTGCCAC CCATGCCGCC AGCACCAGCC CCACGGAGCT CGTACCCTAC 
GGGCTGTCCC TGCGCCTTGG CCGCCTCTGC ACTGAGAACC TTATGAAGCC GGGCACCCGA 
GAGTGTGACC AGCTACGCCA GGAGGTGGAG GAAAATCTGA ATGAGGTGTA TAGACAGGTC 
AGCGGCGCAC ACAAGCTCCA GCAGACGAAG TTCCGACAGC AGCCCAACAC CGGGAAAAAG 
CAAGACCACA CCATTGTGGA CACAGTGTTG CTGGCGCCCC GCTCCGCCAA GCAGATGCTG 
CTGAAGCTGA CAGAGAAGCA GGTGGAGCAG GGGTCCTTCC ATGAACTGAA GGTGGCCCCT 
GGCTACTACA CTGTCACGGC AGAGCAGGAT GCCCGGGGCA TGGTGGAGTT CCAGGAGGGC 
GTGGAGCTGG TGGATGTGCG AGTGCCCCTC TTCATCCGGC CTGAGGATGA TGATGAGAAG 
CAGCTGCTGG TGGAGGCCAT TGATGTCCCT GTGGGCACTG CCACCCTTGG TCGCCGTCTG 
GTAAACATCA CCATTATCAA GGAACAAGCT AGTGGGGTAG TGTCCTTCGA GCAGCCTGAA 
TACTCGGTGA GTCGTGGAGA CCAGGTGGCC CGCATCCCTG TCATCCGGCA CATCCTGGAC 
AATGGCAAGT CCCAGGTCTC CTATAGCACA CAGGATAATA CAGCACACGG ACACCGGGAT 
TATGTCCCTG TGGAGGGAGA GCTGCTGTTC CATCCTGGGG AGACCTGGAA GGAGTTGCAG 
GTGAAGCTAC TGGAGCTGCA GGAGGTCGAC TCCCTCCTGC GTGGCCGCCA GGTCCGCCGC 
TTCCAAGTCC AACTCAGCAA CCCCAAGTTC GGAGCCCGCC TGGGCCAGCC CAGCACAACC 
ACCGTTATTC TCGGTGAGCA CGATGAAACG GACAGGAGTC TCATAAATCA AACACTTTCA 
TCGCCTCCGC CACCCCATGG AGACCTGGGC GCCCCACAGA ACCCCAATGC CAAGGCTGCC 
GGGTCCAGGA AGATCCATTT CAACTGGCTG CCCCCTCCTG GCAAGCCAAT GGGGTACAGG 
GTGAAGTACT GGATCCAGGG CGACTCTGAG TCTGAAGCCC ACCTTCTAGA CAGCAAGGTG 
CCCTCAGTGG AACTCACCAA CCTGTATCCC TATTGCGACT ACGAAATGAA GGTGTGTGCC 
TATGGGGCAC AGGGTGAGGG GCCCTATAGC TCACTGGTGT CCTGCCGCAC CCACCAGGAA 
GTACCCAGCG AGCCAGGGAG GCTGGCTTTC AATGTAGTCT CTTCTACGGT GACGCAGCTG 
AGCTGGGCAG AGCCAGCTGA GACCAATGGC GAGATCACAG CCTACGAGGT CTGCTATGGA 
CTGGTCAATG AGGACAACCG ACCCATTGGA CCTATGAAGA AGGTGCTCGT GGACAACCCC 
AAGAACCGGA TGCTGCTCAT TGAGAATCTG CGAGAATCCC AGCCATACCG ATACACGGTT 
AAGGCGCGCA ATGGGGCAGG ATGGGGACCC GAGAGAGAGG CTATCATCAA CCTGGCTACA 
CAGCCCAAGC GGCCCATGTC CATCCCTATC ATCCCAGACA TCCCCATAGT GGACGCCCAG 
GGTGGAGAAG ACTACGAAAA CTTCCTTATG TACAGTGATG ACGTCCTGCG GTCCCCAGCC 
AGCAGCCAGA GGCCCAGCGT TTCTGATGAC ACTGGCTGCG GCTGGAAGTT CGAGCCTCTG 
CTCGGGGAGG AGCTGGACCT GCGGCGCGTC ACGTGGCGGC TACCCCCGGA ACTCATCCCG 
CGCCTGTCCG CCAGCAGCGG GCGCTCTGAC GAAGACGGCA GCGTTGCGGG GGGCGTGGAA 
GGGGAGGGCA GCGGCTGGAT CCGCGGGGCT ACGCCCCGAC CCCCGGGAGA GCACCTGGTG 
AATGGCCGGA TGGACTTTGC CTATCCAGGC AGCGCCAACT CCCTGCACAG AATGACTGCA 
GCCAATGTGG CCTATGGCAC GCATCTGAGC CCACACCTGT CCCACCGAGT GCTGAGCACG 
TCCTCCACCC TTACCCGGGA CTACCACTCT CTGACACGCA CAGAGCACTC CCACTCAGGC 
ACACTTCCCA GGGACTACTC CACCCTCACT TCCCTTTCCT CCCAAGGCCT CCCTCCTATC 
TGGGAAGATG GGAGGAGCAG GCTTCCGCTG TCCTGGACTC TTGGGTCCTT GAGCCGGGCT 
CACATGAAGG GTGTGCCCGC ATCCAGGGGT TCACCAGACT CTATAATCCT GGCCGGGCAG 
TCAGCAGCAC CCTCCTGGGG TACAGATTCC CGTGGGGCTG TGGGTGTGCC TGACACACCC 
ACTCGGCTGG TGTTCTCTGC CCTGGGGCCC ACGTCTTTGA AGGTGAGCTG GCAGGAGCCA 
CAGTGTGATC GGATGCTGCT GGGCTACAGT GTGGAATACC AGCTACTAAA CGGCGGCGAG 
ATGCATCGGC TCAACATCCC TAACCCTGGC CAAACCTCGG TGGTGGTAGA GGATCTCCTG 
CCTAACCACT CCTATGTGTT CCGGGTACGG GCCCAGAGCC AGGAGGGCTG GGGCCGAGAG 
CGAGAGGGTG TCATCACCAT CGAGTCCCAG GTGCACCCGC AGAGCCCTCT CTGCCCCCTG 
CCAGGCTCAG CCTTCACTCT GAGCACCCCC AGCGCCCCGG GACCGCTGGT GTTCACTGCC 
CTAAGCCCAG ACTCCCTGCA GCTCAGCTGG GAGCGGCCGA GGAGGCCCAA TGGAGATATC 
CTTGGCTACC TGGTGACCTG TGAGATGGCC CAAGGAGGAG CACCAGCCAG GACCTTCCGG 
GTGGACGGAG ACAACCCTGA GAGCCGGTTG ACTGTACCTG GCCTCAGTGA GAACGTTCCT 
TACAAGTTCA AGGTTCAGGC CAGGACGACC GAGGGCTTCG GGCCAGAGCG TGAGGGTATC 
ATCACCATCG AGTCTCAGGT TGGAGGCCCC TTCCCACAGC TGGGCAGCCA TTCTGGGCTC 
TTCCAGAACC CAGTGCAAAG CGAGTTCAGC AGCGTGACCA GCACGCACAG CACCACGACT 
GAGCCCTTCC TCATGGATGG TCTAACCCTG GGGACCCAGC GCCTGGAAGC AGGAGGCTCC 
CTCACCCGGC ATGTGACCCA GGAATTCGTG ACCCGGACCT TAACGGCCAG TGGCTCTCTC 
AGCACTCATA TGGACCAACA GTTCTTCCAA ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itgb4 ( XM_017314350.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169839.1 Mus musculus integrin beta 4 (Itgb4), transcript variant X2, mRNA.

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CloneID	OMu77782
Clone ID Related Accession (Same CDS sequence)	XM_017314350.1 , XM_017314350.2
Accession Version	XM_017314350.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	integrin beta-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGC CCTGTTGCAG CCCATGGGTG AAGCTGCTGC TGCTGGCAGC AATGCTGAGT 
GCCAGCCTCC CTGGAGACCT GGCCAACCGC TGCAAGAAGG CTCAGGTGAA GAGCTGTACC 
GAGTGCATCC GGGTGGACAA GAGCTGTGCC TACTGCACAG ACGAGCTGTT CAAGGAGAGG 
CGCTGCAACA CCCAGGCGGA GCTTCTGGCT GCAGGCTGCA GGGGAGAGAG CATCCTGGTC 
ATGGAGAGCA GCCTTGAAAT CACAGAGAAC ACCCAGATCG ACACCAGCCT GCACCGCAGC 
CAGGTATCTC CCCAAGGCCT GCAAGTCCGG CTGCGGCCGG GTGAGGAGCG CAGCTTTGTG 
TTCCAGGTGT TTGAGCCCCT GGAGAGCCCC GTGGATCTGT ATATCCTCAT GGACTTCTCC 
AACTCCATGT CTGACGATCT GGACAACCTC AAGCAGATGG GGCAGAACCT GGCCAAGATC 
CTGCGCCAGC TCACCAGCGA CTACACCATT GGATTTGGAA AGTTTGTGGA CAAAGTCAGC 
GTCCCACAGA CAGACATGAG GCCCGAGAAA CTGAAGGAGC CCTGGCCCAA CAGTGATCCC 
CCGTTCTCCT TCAAGAACGT TATCAGCTTA ACGGAGAATG TGGAAGAATT CTGGAACAAA 
CTGCAAGGAG AACGCATCTC AGGCAACCTG GACGCTCCTG AAGGGGGCTT TGATGCCATC 
CTGCAGACAG CTGTGTGCAC AAGGGACATT GGCTGGAGGG CTGACAGCAC CCACCTGCTG 
GTGTTCTCCA CCGAGTCTGC CTTCCACTAC GAGGCTGATG GTGCCAACGT GCTGGCCGGC 
ATCATGAACC GCAATGATGA GAAATGCCAC CTGGACGCCT CGGGCGCCTA CACCCAATAC 
AAGACACAGG ACTACCCATC AGTGCCCACG CTGGTTCGCC TGCTTGCCAA GCATAACATC 
ATCCCCATCT TTGCTGTCAC CAACTACTCT TACAGCTACT ATGAGAAGCT CCATAAGTAT 
TTCCCCGTCT CCTCTCTGGG CGTCCTGCAG GAGGATTCAT CCAACATCGT GGAGCTGCTG 
GAGGAGGCCT TCTATCGAAT TCGCTCCAAC CTGGACATCC GGGCTCTGGA CAGCCCCAGA 
GGCCTGAGAA CAGAGGTCAC CTCCGATACT CTCCAGAAGA CGGAGACTGG GTCCTTTCAC 
ATCAAGCGGG GGGAAGTGGG CACATACAAT GTGCATCTCC GGGCAGTGGA GGACATAGAT 
GGGACACATG TGTGCCAGCT GGCTAAAGAA GACCAAGGGG GCAACATCCA CCTGAAACCC 
TCCTTCTCTG ATGGCCTCCG GATGGACGCG AGTGTGATCT GTGACGTGTG CCCCTGTGAG 
CTGCAAAAGG AAGTTCGATC AGCTCGCTGT CACTTCAGAG GAGACTTCAT GTGTGGACAC 
TGTGTGTGCA ATGAGGGCTG GAGTGGCAAA ACCTGCAACT GCTCCACCGG CTCTCTGAGT 
GACACACAGC CCTGCCTGCG TGAGGGTGAG GACAAACCGT GCTCGGGCCA CGGCGAGTGC 
CAGTGCGGAC GCTGTGTGTG CTATGGTGAA GGCCGCTACG AGGGTCACTT CTGCGAGTAT 
GACAACTTCC AGTGTCCCCG GACCTCTGGA TTCCTGTGCA ATGACCGGGG TCGCTGTTCT 
ATGGGAGAGT GTGTGTGTGA GCCTGGTTGG ACAGGCCGCA GCTGTGACTG TCCCCTCAGC 
AATGCCACCT GCATCGATAG CAACGGGGGC ATCTGCAACG GCCGAGGCTA CTGTGAGTGT 
GGCCGTTGTC ACTGCAACCA GCAGTCGCTC TACACGGACA CCACCTGTGA GATCAACTAC 
TCTGCGATAC GCCTGGGTCT CTGTGAGGAT CTCCGCTCCT GCGTACAGTG CCAGGCCTGG 
GGCACCGGGG AGAAGAAAGG GCGCGCGTGT GACGATTGCC CCTTTAAAGT CAAGATGGTA 
GACGAGCTTA AGAAAGAAGA GGTGGTGGAG TACTGCTCCT TCCGGGATGA GGATGACGAC 
TGCACTTACA GCTACAACGT GGAGGGCGAT GGCAGCCCTG GGCCCAACAG CACAGTCCTG 
GTCCACAAAA AGAAAGACTG CCCTCCCGGC TCCTTCTGGT GGCTCATCCC CCTGCTCATC 
TTCCTCCTGT TGCTCCTGGC GTTGCTTCTG CTGCTCTGCT GGAAATACTG TGCCTGCTGC 
AAAGCCTGCC TGGGGCTTCT TCCTTGCTGC AACCGAGGTC ACATGGTGGG CTTTAAGGAA 
GATCACTATA TGCTTCGGGA GAACCTGATG GCCTCTGACC ACCTGGACAC GCCCATGCTA 
CGAAGCGGGA ACCTCAAGGG ACGAGACACA GTCCGCTGGA AGATCACCAA CAATGTGCAG 
CGCCCTGGCT TTGCCACCCA TGCCGCCAGC ACCAGCCCCA CGGAGCTCGT ACCCTACGGG 
CTGTCCCTGC GCCTTGGCCG CCTCTGCACT GAGAACCTTA TGAAGCCGGG CACCCGAGAG 
TGTGACCAGC TACGCCAGGA GGTGGAGGAA AATCTGAATG AGGTGTATAG ACAGGTCAGC 
GGCGCACACA AGCTCCAGCA GACGAAGTTC CGACAGCAGC CCAACACCGG GAAAAAGCAA 
GACCACACCA TTGTGGACAC AGTGTTGCTG GCGCCCCGCT CCGCCAAGCA GATGCTGCTG 
AAGCTGACAG AGAAGCAGGT GGAGCAGGGG TCCTTCCATG AACTGAAGGT GGCCCCTGGC 
TACTACACTG TCACGGCAGA GCAGGATGCC CGGGGCATGG TGGAGTTCCA GGAGGGCGTG 
GAGCTGGTGG ATGTGCGAGT GCCCCTCTTC ATCCGGCCTG AGGATGATGA TGAGAAGCAG 
CTGCTGGTGG AGGCCATTGA TGTCCCTGTG GGCACTGCCA CCCTTGGTCG CCGTCTGGTA 
AACATCACCA TTATCAAGGA ACAAGCTAGT GGGGTAGTGT CCTTCGAGCA GCCTGAATAC 
TCGGTGAGTC GTGGAGACCA GGTGGCCCGC ATCCCTGTCA TCCGGCACAT CCTGGACAAT 
GGCAAGTCCC AGGTCTCCTA TAGCACACAG GATAATACAG CACACGGACA CCGGGATTAT 
GTCCCTGTGG AGGGAGAGCT GCTGTTCCAT CCTGGGGAGA CCTGGAAGGA GTTGCAGGTG 
AAGCTACTGG AGCTGCAGGA GGTCGACTCC CTCCTGCGTG GCCGCCAGGT CCGCCGCTTC 
CAAGTCCAAC TCAGCAACCC CAAGTTCGGA GCCCGCCTGG GCCAGCCCAG CACAACCACC 
GTTATTCTCG GTGAGCACGA TGAAACGGAC AGGAGTCTCA TAAATCAAAC ACTTTCATCG 
CCTCCGCCAC CCCATGGAGA CCTGGGCGCC CCACAGAACC CCAATGCCAA GGCTGCCGGG 
TCCAGGAAGA TCCATTTCAA CTGGCTGCCC CCTCCTGGCA AGCCAATGGG GTACAGGGTG 
AAGTACTGGA TCCAGGGCGA CTCTGAGTCT GAAGCCCACC TTCTAGACAG CAAGGTGCCC 
TCAGTGGAAC TCACCAACCT GTATCCCTAT TGCGACTACG AAATGAAGGT GTGTGCCTAT 
GGGGCACAGG GTGAGGGGCC CTATAGCTCA CTGGTGTCCT GCCGCACCCA CCAGGAAGTA 
CCCAGCGAGC CAGGGAGGCT GGCTTTCAAT GTAGTCTCTT CTACGGTGAC GCAGCTGAGC 
TGGGCAGAGC CAGCTGAGAC CAATGGCGAG ATCACAGCCT ACGAGGTCTG CTATGGACTG 
GTCAATGAGG ACAACCGACC CATTGGACCT ATGAAGAAGG TGCTCGTGGA CAACCCCAAG 
AACCGGATGC TGCTCATTGA GAATCTGCGA GAATCCCAGC CATACCGATA CACGGTTAAG 
GCGCGCAATG GGGCAGGATG GGGACCCGAG AGAGAGGCTA TCATCAACCT GGCTACACAG 
CCCAAGCGGC CCATGTCCAT CCCTATCATC CCAGACATCC CCATAGTGGA CGCCCAGGGT 
GGAGAAGACT ACGAAAACTT CCTTATGTAC AGTGATGACG TCCTGCGGTC CCCAGCCAGC 
AGCCAGAGGC CCAGCGTTTC TGATGACACT GGCTGCGGCT GGAAGTTCGA GCCTCTGCTC 
GGGGAGGAGC TGGACCTGCG GCGCGTCACG TGGCGGCTAC CCCCGGAACT CATCCCGCGC 
CTGTCCGCCA GCAGCGGGCG CTCTGACGAA GACGGCAGCG TTGCGGGGGG CGTGGAAGGG 
GAGGGCAGCG GCTGGATCCG CGGGGCTACG CCCCGACCCC CGGGAGAGCA CCTGGTGAAT 
GGCCGGATGG ACTTTGCCTA TCCAGGCAGC GCCAACTCCC TGCACAGAAT GACTGCAGCC 
AATGTGGCCT ATGGCACGCA TCTGAGCCCA CACCTGTCCC ACCGAGTGCT GAGCACGTCC 
TCCACCCTTA CCCGGGACTA CCACTCTCTG ACACGCACAG AGCACTCCCA CTCAGGCACA 
CTTCCCAGGG ACTACTCCAC CCTCACTTCC CTTTCCTCCC AAGGCCTCCC TCCTATCTGG 
GAAGATGGGA GGAGCAGGCT TCCGCTGTCC TGGACTCTTG GGTCCTTGAG CCGGGCTCAC 
ATGAAGGGTG TGCCCGCATC CAGGGGTTCA CCAGACTCTA TAATCCTGGC CGGGCAGTCA 
GCAGCACCCT CCTGGGGTAC AGATTCCCGT GGGGCTGTGG GTGTGCCTGA CACACCCACT 
CGGCTGGTGT TCTCTGCCCT GGGGCCCACG TCTTTGAAGG TGAGCTGGCA GGAGCCACAG 
TGTGATCGGA TGCTGCTGGG CTACAGTGTG GAATACCAGC TACTAAACGG CGGCGAGATG 
CATCGGCTCA ACATCCCTAA CCCTGGCCAA ACCTCGGTGG TGGTAGAGGA TCTCCTGCCT 
AACCACTCCT ATGTGTTCCG GGTACGGGCC CAGAGCCAGG AGGGCTGGGG CCGAGAGCGA 
GAGGGTGTCA TCACCATCGA GTCCCAGGTG CACCCGCAGA GCCCTCTCTG CCCCCTGCCA 
GGCTCAGCCT TCACTCTGAG CACCCCCAGC GCCCCGGGAC CGCTGGTGTT CACTGCCCTA 
AGCCCAGACT CCCTGCAGCT CAGCTGGGAG CGGCCGAGGA GGCCCAATGG AGATATCCTT 
GGCTACCTGG TGACCTGTGA GATGGCCCAA GGAGGAGCAC CAGCCAGGAC CTTCCGGGTG 
GACGGAGACA ACCCTGAGAG CCGGTTGACT GTACCTGGCC TCAGTGAGAA CGTTCCTTAC 
AAGTTCAAGG TTCAGGCCAG GACGACCGAG GGCTTCGGGC CAGAGCGTGA GGGTATCATC 
ACCATCGAGT CTCAGGTTGG AGGCCCCTTC CCACAGCTGG GCAGCCATTC TGGGCTCTTC 
CAGAACCCAG TGCAAAGCGA GTTCAGCAGC GTGACCAGCA CGCACAGCAC CACGACTGAG 
CCCTTCCTCA TGGATGGTCT AACCCTGGGG ACCCAGCGCC TGGAAGCAGG AGGCTCCCTC 
ACCCGGCATG TGACCCAGGA ATTCGTGACC CGGACCTTAA CGGCCAGTGG CTCTCTCAGC 
ACTCATATGG ACCAACAGTT CTTCCAAACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169839.1
CDS	436..6048
Translation	MAGPCCSPWVKLLLLAAMLSASLPGDLANRCKKAQVKSCTECIRVDKSCAYCTDELFKERRCNTQAELLAAGCRGESILVMESSLEITENTQIDTSLHRSQVSPQGLQVRLRPGEERSFVFQVFEPLESPVDLYILMDFSNSMSDDLDNLKQMGQNLAKILRQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTENVEEFWNKLQGERISGNLDAPEGGFDAILQTAVCTRDIGWRADSTHLLVFSTESAFHYEADGANVLAGIMNRNDEKCHLDASGAYTQYKTQDYPSVPTLVRLLAKHNIIPIFAVTNYSYSYYEKLHKYFPVSSLGVLQEDSSNIVELLEEAFYRIRSNLDIRALDSPRGLRTEVTSDTLQKTETGSFHIKRGEVGTYNVHLRAVEDIDGTHVCQLAKEDQGGNIHLKPSFSDGLRMDASVICDVCPCELQKEVRSARCHFRGDFMCGHCVCNEGWSGKTCNCSTGSLSDTQPCLREGEDKPCSGHGECQCGRCVCYGEGRYEGHFCEYDNFQCPRTSGFLCNDRGRCSMGECVCEPGWTGRSCDCPLSNATCIDSNGGICNGRGYCECGRCHCNQQSLYTDTTCEINYSAIRLGLCEDLRSCVQCQAWGTGEKKGRACDDCPFKVKMVDELKKEEVVEYCSFRDEDDDCTYSYNVEGDGSPGPNSTVLVHKKKDCPPGSFWWLIPLLIFLLLLLALLLLLCWKYCACCKACLGLLPCCNRGHMVGFKEDHYMLRENLMASDHLDTPMLRSGNLKGRDTVRWKITNNVQRPGFATHAASTSPTELVPYGLSLRLGRLCTENLMKPGTRECDQLRQEVEENLNEVYRQVSGAHKLQQTKFRQQPNTGKKQDHTIVDTVLLAPRSAKQMLLKLTEKQVEQGSFHELKVAPGYYTVTAEQDARGMVEFQEGVELVDVRVPLFIRPEDDDEKQLLVEAIDVPVGTATLGRRLVNITIIKEQASGVVSFEQPEYSVSRGDQVARIPVIRHILDNGKSQVSYSTQDNTAHGHRDYVPVEGELLFHPGETWKELQVKLLELQEVDSLLRGRQVRRFQVQLSNPKFGARLGQPSTTTVILGEHDETDRSLINQTLSSPPPPHGDLGAPQNPNAKAAGSRKIHFNWLPPPGKPMGYRVKYWIQGDSESEAHLLDSKVPSVELTNLYPYCDYEMKVCAYGAQGEGPYSSLVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYGLVNEDNRPIGPMKKVLVDNPKNRMLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDIPIVDAQGGEDYENFLMYSDDVLRSPASSQRPSVSDDTGCGWKFEPLLGEELDLRRVTWRLPPELIPRLSASSGRSDEDGSVAGGVEGEGSGWIRGATPRPPGEHLVNGRMDFAYPGSANSLHRMTAANVAYGTHLSPHLSHRVLSTSSTLTRDYHSLTRTEHSHSGTLPRDYSTLTSLSSQGLPPIWEDGRSRLPLSWTLGSLSRAHMKGVPASRGSPDSIILAGQSAAPSWGTDSRGAVGVPDTPTRLVFSALGPTSLKVSWQEPQCDRMLLGYSVEYQLLNGGEMHRLNIPNPGQTSVVVEDLLPNHSYVFRVRAQSQEGWGREREGVITIESQVHPQSPLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPNGDILGYLVTCEMAQGGAPARTFRVDGDNPESRLTVPGLSENVPYKFKVQARTTEGFGPEREGIITIESQVGGPFPQLGSHSGLFQNPVQSEFSSVTSTHSTTTEPFLMDGLTLGTQRLEAGGSLTRHVTQEFVTRTLTASGSLSTHMDQQFFQT

Target ORF information:

RefSeq Version	XM_017314350.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus integrin beta 4 (Itgb4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314350.1

ORF Insert Sequence:

ATGGCAGGGC CCTGTTGCAG CCCATGGGTG AAGCTGCTGC TGCTGGCAGC AATGCTGAGT 
GCCAGCCTCC CTGGAGACCT GGCCAACCGC TGCAAGAAGG CTCAGGTGAA GAGCTGTACC 
GAGTGCATCC GGGTGGACAA GAGCTGTGCC TACTGCACAG ACGAGCTGTT CAAGGAGAGG 
CGCTGCAACA CCCAGGCGGA GCTTCTGGCT GCAGGCTGCA GGGGAGAGAG CATCCTGGTC 
ATGGAGAGCA GCCTTGAAAT CACAGAGAAC ACCCAGATCG ACACCAGCCT GCACCGCAGC 
CAGGTATCTC CCCAAGGCCT GCAAGTCCGG CTGCGGCCGG GTGAGGAGCG CAGCTTTGTG 
TTCCAGGTGT TTGAGCCCCT GGAGAGCCCC GTGGATCTGT ATATCCTCAT GGACTTCTCC 
AACTCCATGT CTGACGATCT GGACAACCTC AAGCAGATGG GGCAGAACCT GGCCAAGATC 
CTGCGCCAGC TCACCAGCGA CTACACCATT GGATTTGGAA AGTTTGTGGA CAAAGTCAGC 
GTCCCACAGA CAGACATGAG GCCCGAGAAA CTGAAGGAGC CCTGGCCCAA CAGTGATCCC 
CCGTTCTCCT TCAAGAACGT TATCAGCTTA ACGGAGAATG TGGAAGAATT CTGGAACAAA 
CTGCAAGGAG AACGCATCTC AGGCAACCTG GACGCTCCTG AAGGGGGCTT TGATGCCATC 
CTGCAGACAG CTGTGTGCAC AAGGGACATT GGCTGGAGGG CTGACAGCAC CCACCTGCTG 
GTGTTCTCCA CCGAGTCTGC CTTCCACTAC GAGGCTGATG GTGCCAACGT GCTGGCCGGC 
ATCATGAACC GCAATGATGA GAAATGCCAC CTGGACGCCT CGGGCGCCTA CACCCAATAC 
AAGACACAGG ACTACCCATC AGTGCCCACG CTGGTTCGCC TGCTTGCCAA GCATAACATC 
ATCCCCATCT TTGCTGTCAC CAACTACTCT TACAGCTACT ATGAGAAGCT CCATAAGTAT 
TTCCCCGTCT CCTCTCTGGG CGTCCTGCAG GAGGATTCAT CCAACATCGT GGAGCTGCTG 
GAGGAGGCCT TCTATCGAAT TCGCTCCAAC CTGGACATCC GGGCTCTGGA CAGCCCCAGA 
GGCCTGAGAA CAGAGGTCAC CTCCGATACT CTCCAGAAGA CGGAGACTGG GTCCTTTCAC 
ATCAAGCGGG GGGAAGTGGG CACATACAAT GTGCATCTCC GGGCAGTGGA GGACATAGAT 
GGGACACATG TGTGCCAGCT GGCTAAAGAA GACCAAGGGG GCAACATCCA CCTGAAACCC 
TCCTTCTCTG ATGGCCTCCG GATGGACGCG AGTGTGATCT GTGACGTGTG CCCCTGTGAG 
CTGCAAAAGG AAGTTCGATC AGCTCGCTGT CACTTCAGAG GAGACTTCAT GTGTGGACAC 
TGTGTGTGCA ATGAGGGCTG GAGTGGCAAA ACCTGCAACT GCTCCACCGG CTCTCTGAGT 
GACACACAGC CCTGCCTGCG TGAGGGTGAG GACAAACCGT GCTCGGGCCA CGGCGAGTGC 
CAGTGCGGAC GCTGTGTGTG CTATGGTGAA GGCCGCTACG AGGGTCACTT CTGCGAGTAT 
GACAACTTCC AGTGTCCCCG GACCTCTGGA TTCCTGTGCA ATGACCGGGG TCGCTGTTCT 
ATGGGAGAGT GTGTGTGTGA GCCTGGTTGG ACAGGCCGCA GCTGTGACTG TCCCCTCAGC 
AATGCCACCT GCATCGATAG CAACGGGGGC ATCTGCAACG GCCGAGGCTA CTGTGAGTGT 
GGCCGTTGTC ACTGCAACCA GCAGTCGCTC TACACGGACA CCACCTGTGA GATCAACTAC 
TCTGCGATAC GCCTGGGTCT CTGTGAGGAT CTCCGCTCCT GCGTACAGTG CCAGGCCTGG 
GGCACCGGGG AGAAGAAAGG GCGCGCGTGT GACGATTGCC CCTTTAAAGT CAAGATGGTA 
GACGAGCTTA AGAAAGAAGA GGTGGTGGAG TACTGCTCCT TCCGGGATGA GGATGACGAC 
TGCACTTACA GCTACAACGT GGAGGGCGAT GGCAGCCCTG GGCCCAACAG CACAGTCCTG 
GTCCACAAAA AGAAAGACTG CCCTCCCGGC TCCTTCTGGT GGCTCATCCC CCTGCTCATC 
TTCCTCCTGT TGCTCCTGGC GTTGCTTCTG CTGCTCTGCT GGAAATACTG TGCCTGCTGC 
AAAGCCTGCC TGGGGCTTCT TCCTTGCTGC AACCGAGGTC ACATGGTGGG CTTTAAGGAA 
GATCACTATA TGCTTCGGGA GAACCTGATG GCCTCTGACC ACCTGGACAC GCCCATGCTA 
CGAAGCGGGA ACCTCAAGGG ACGAGACACA GTCCGCTGGA AGATCACCAA CAATGTGCAG 
CGCCCTGGCT TTGCCACCCA TGCCGCCAGC ACCAGCCCCA CGGAGCTCGT ACCCTACGGG 
CTGTCCCTGC GCCTTGGCCG CCTCTGCACT GAGAACCTTA TGAAGCCGGG CACCCGAGAG 
TGTGACCAGC TACGCCAGGA GGTGGAGGAA AATCTGAATG AGGTGTATAG ACAGGTCAGC 
GGCGCACACA AGCTCCAGCA GACGAAGTTC CGACAGCAGC CCAACACCGG GAAAAAGCAA 
GACCACACCA TTGTGGACAC AGTGTTGCTG GCGCCCCGCT CCGCCAAGCA GATGCTGCTG 
AAGCTGACAG AGAAGCAGGT GGAGCAGGGG TCCTTCCATG AACTGAAGGT GGCCCCTGGC 
TACTACACTG TCACGGCAGA GCAGGATGCC CGGGGCATGG TGGAGTTCCA GGAGGGCGTG 
GAGCTGGTGG ATGTGCGAGT GCCCCTCTTC ATCCGGCCTG AGGATGATGA TGAGAAGCAG 
CTGCTGGTGG AGGCCATTGA TGTCCCTGTG GGCACTGCCA CCCTTGGTCG CCGTCTGGTA 
AACATCACCA TTATCAAGGA ACAAGCTAGT GGGGTAGTGT CCTTCGAGCA GCCTGAATAC 
TCGGTGAGTC GTGGAGACCA GGTGGCCCGC ATCCCTGTCA TCCGGCACAT CCTGGACAAT 
GGCAAGTCCC AGGTCTCCTA TAGCACACAG GATAATACAG CACACGGACA CCGGGATTAT 
GTCCCTGTGG AGGGAGAGCT GCTGTTCCAT CCTGGGGAGA CCTGGAAGGA GTTGCAGGTG 
AAGCTACTGG AGCTGCAGGA GGTCGACTCC CTCCTGCGTG GCCGCCAGGT CCGCCGCTTC 
CAAGTCCAAC TCAGCAACCC CAAGTTCGGA GCCCGCCTGG GCCAGCCCAG CACAACCACC 
GTTATTCTCG GTGAGCACGA TGAAACGGAC AGGAGTCTCA TAAATCAAAC ACTTTCATCG 
CCTCCGCCAC CCCATGGAGA CCTGGGCGCC CCACAGAACC CCAATGCCAA GGCTGCCGGG 
TCCAGGAAGA TCCATTTCAA CTGGCTGCCC CCTCCTGGCA AGCCAATGGG GTACAGGGTG 
AAGTACTGGA TCCAGGGCGA CTCTGAGTCT GAAGCCCACC TTCTAGACAG CAAGGTGCCC 
TCAGTGGAAC TCACCAACCT GTATCCCTAT TGCGACTACG AAATGAAGGT GTGTGCCTAT 
GGGGCACAGG GTGAGGGGCC CTATAGCTCA CTGGTGTCCT GCCGCACCCA CCAGGAAGTA 
CCCAGCGAGC CAGGGAGGCT GGCTTTCAAT GTAGTCTCTT CTACGGTGAC GCAGCTGAGC 
TGGGCAGAGC CAGCTGAGAC CAATGGCGAG ATCACAGCCT ACGAGGTCTG CTATGGACTG 
GTCAATGAGG ACAACCGACC CATTGGACCT ATGAAGAAGG TGCTCGTGGA CAACCCCAAG 
AACCGGATGC TGCTCATTGA GAATCTGCGA GAATCCCAGC CATACCGATA CACGGTTAAG 
GCGCGCAATG GGGCAGGATG GGGACCCGAG AGAGAGGCTA TCATCAACCT GGCTACACAG 
CCCAAGCGGC CCATGTCCAT CCCTATCATC CCAGACATCC CCATAGTGGA CGCCCAGGGT 
GGAGAAGACT ACGAAAACTT CCTTATGTAC AGTGATGACG TCCTGCGGTC CCCAGCCAGC 
AGCCAGAGGC CCAGCGTTTC TGATGACACT GGCTGCGGCT GGAAGTTCGA GCCTCTGCTC 
GGGGAGGAGC TGGACCTGCG GCGCGTCACG TGGCGGCTAC CCCCGGAACT CATCCCGCGC 
CTGTCCGCCA GCAGCGGGCG CTCTGACGAA GACGGCAGCG TTGCGGGGGG CGTGGAAGGG 
GAGGGCAGCG GCTGGATCCG CGGGGCTACG CCCCGACCCC CGGGAGAGCA CCTGGTGAAT 
GGCCGGATGG ACTTTGCCTA TCCAGGCAGC GCCAACTCCC TGCACAGAAT GACTGCAGCC 
AATGTGGCCT ATGGCACGCA TCTGAGCCCA CACCTGTCCC ACCGAGTGCT GAGCACGTCC 
TCCACCCTTA CCCGGGACTA CCACTCTCTG ACACGCACAG AGCACTCCCA CTCAGGCACA 
CTTCCCAGGG ACTACTCCAC CCTCACTTCC CTTTCCTCCC AAGGCCTCCC TCCTATCTGG 
GAAGATGGGA GGAGCAGGCT TCCGCTGTCC TGGACTCTTG GGTCCTTGAG CCGGGCTCAC 
ATGAAGGGTG TGCCCGCATC CAGGGGTTCA CCAGACTCTA TAATCCTGGC CGGGCAGTCA 
GCAGCACCCT CCTGGGGTAC AGATTCCCGT GGGGCTGTGG GTGTGCCTGA CACACCCACT 
CGGCTGGTGT TCTCTGCCCT GGGGCCCACG TCTTTGAAGG TGAGCTGGCA GGAGCCACAG 
TGTGATCGGA TGCTGCTGGG CTACAGTGTG GAATACCAGC TACTAAACGG CGGCGAGATG 
CATCGGCTCA ACATCCCTAA CCCTGGCCAA ACCTCGGTGG TGGTAGAGGA TCTCCTGCCT 
AACCACTCCT ATGTGTTCCG GGTACGGGCC CAGAGCCAGG AGGGCTGGGG CCGAGAGCGA 
GAGGGTGTCA TCACCATCGA GTCCCAGGTG CACCCGCAGA GCCCTCTCTG CCCCCTGCCA 
GGCTCAGCCT TCACTCTGAG CACCCCCAGC GCCCCGGGAC CGCTGGTGTT CACTGCCCTA 
AGCCCAGACT CCCTGCAGCT CAGCTGGGAG CGGCCGAGGA GGCCCAATGG AGATATCCTT 
GGCTACCTGG TGACCTGTGA GATGGCCCAA GGAGGAGCAC CAGCCAGGAC CTTCCGGGTG 
GACGGAGACA ACCCTGAGAG CCGGTTGACT GTACCTGGCC TCAGTGAGAA CGTTCCTTAC 
AAGTTCAAGG TTCAGGCCAG GACGACCGAG GGCTTCGGGC CAGAGCGTGA GGGTATCATC 
ACCATCGAGT CTCAGGTTGG AGGCCCCTTC CCACAGCTGG GCAGCCATTC TGGGCTCTTC 
CAGAACCCAG TGCAAAGCGA GTTCAGCAGC GTGACCAGCA CGCACAGCAC CACGACTGAG 
CCCTTCCTCA TGGATGGTCT AACCCTGGGG ACCCAGCGCC TGGAAGCAGG AGGCTCCCTC 
ACCCGGCATG TGACCCAGGA ATTCGTGACC CGGACCTTAA CGGCCAGTGG CTCTCTCAGC 
ACTCATATGG ACCAACAGTT CTTCCAAACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itgb4 ( XM_017314350.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169839.1 Mus musculus integrin beta 4 (Itgb4), transcript variant X2, mRNA.

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CloneID	OMu77782
Clone ID Related Accession (Same CDS sequence)	XM_017314350.1 , XM_017314350.2
Accession Version	XM_017314350.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	integrin beta-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314350.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGC CCTGTTGCAG CCCATGGGTG AAGCTGCTGC TGCTGGCAGC AATGCTGAGT 
GCCAGCCTCC CTGGAGACCT GGCCAACCGC TGCAAGAAGG CTCAGGTGAA GAGCTGTACC 
GAGTGCATCC GGGTGGACAA GAGCTGTGCC TACTGCACAG ACGAGCTGTT CAAGGAGAGG 
CGCTGCAACA CCCAGGCGGA GCTTCTGGCT GCAGGCTGCA GGGGAGAGAG CATCCTGGTC 
ATGGAGAGCA GCCTTGAAAT CACAGAGAAC ACCCAGATCG ACACCAGCCT GCACCGCAGC 
CAGGTATCTC CCCAAGGCCT GCAAGTCCGG CTGCGGCCGG GTGAGGAGCG CAGCTTTGTG 
TTCCAGGTGT TTGAGCCCCT GGAGAGCCCC GTGGATCTGT ATATCCTCAT GGACTTCTCC 
AACTCCATGT CTGACGATCT GGACAACCTC AAGCAGATGG GGCAGAACCT GGCCAAGATC 
CTGCGCCAGC TCACCAGCGA CTACACCATT GGATTTGGAA AGTTTGTGGA CAAAGTCAGC 
GTCCCACAGA CAGACATGAG GCCCGAGAAA CTGAAGGAGC CCTGGCCCAA CAGTGATCCC 
CCGTTCTCCT TCAAGAACGT TATCAGCTTA ACGGAGAATG TGGAAGAATT CTGGAACAAA 
CTGCAAGGAG AACGCATCTC AGGCAACCTG GACGCTCCTG AAGGGGGCTT TGATGCCATC 
CTGCAGACAG CTGTGTGCAC AAGGGACATT GGCTGGAGGG CTGACAGCAC CCACCTGCTG 
GTGTTCTCCA CCGAGTCTGC CTTCCACTAC GAGGCTGATG GTGCCAACGT GCTGGCCGGC 
ATCATGAACC GCAATGATGA GAAATGCCAC CTGGACGCCT CGGGCGCCTA CACCCAATAC 
AAGACACAGG ACTACCCATC AGTGCCCACG CTGGTTCGCC TGCTTGCCAA GCATAACATC 
ATCCCCATCT TTGCTGTCAC CAACTACTCT TACAGCTACT ATGAGAAGCT CCATAAGTAT 
TTCCCCGTCT CCTCTCTGGG CGTCCTGCAG GAGGATTCAT CCAACATCGT GGAGCTGCTG 
GAGGAGGCCT TCTATCGAAT TCGCTCCAAC CTGGACATCC GGGCTCTGGA CAGCCCCAGA 
GGCCTGAGAA CAGAGGTCAC CTCCGATACT CTCCAGAAGA CGGAGACTGG GTCCTTTCAC 
ATCAAGCGGG GGGAAGTGGG CACATACAAT GTGCATCTCC GGGCAGTGGA GGACATAGAT 
GGGACACATG TGTGCCAGCT GGCTAAAGAA GACCAAGGGG GCAACATCCA CCTGAAACCC 
TCCTTCTCTG ATGGCCTCCG GATGGACGCG AGTGTGATCT GTGACGTGTG CCCCTGTGAG 
CTGCAAAAGG AAGTTCGATC AGCTCGCTGT CACTTCAGAG GAGACTTCAT GTGTGGACAC 
TGTGTGTGCA ATGAGGGCTG GAGTGGCAAA ACCTGCAACT GCTCCACCGG CTCTCTGAGT 
GACACACAGC CCTGCCTGCG TGAGGGTGAG GACAAACCGT GCTCGGGCCA CGGCGAGTGC 
CAGTGCGGAC GCTGTGTGTG CTATGGTGAA GGCCGCTACG AGGGTCACTT CTGCGAGTAT 
GACAACTTCC AGTGTCCCCG GACCTCTGGA TTCCTGTGCA ATGACCGGGG TCGCTGTTCT 
ATGGGAGAGT GTGTGTGTGA GCCTGGTTGG ACAGGCCGCA GCTGTGACTG TCCCCTCAGC 
AATGCCACCT GCATCGATAG CAACGGGGGC ATCTGCAACG GCCGAGGCTA CTGTGAGTGT 
GGCCGTTGTC ACTGCAACCA GCAGTCGCTC TACACGGACA CCACCTGTGA GATCAACTAC 
TCTGCGATAC GCCTGGGTCT CTGTGAGGAT CTCCGCTCCT GCGTACAGTG CCAGGCCTGG 
GGCACCGGGG AGAAGAAAGG GCGCGCGTGT GACGATTGCC CCTTTAAAGT CAAGATGGTA 
GACGAGCTTA AGAAAGAAGA GGTGGTGGAG TACTGCTCCT TCCGGGATGA GGATGACGAC 
TGCACTTACA GCTACAACGT GGAGGGCGAT GGCAGCCCTG GGCCCAACAG CACAGTCCTG 
GTCCACAAAA AGAAAGACTG CCCTCCCGGC TCCTTCTGGT GGCTCATCCC CCTGCTCATC 
TTCCTCCTGT TGCTCCTGGC GTTGCTTCTG CTGCTCTGCT GGAAATACTG TGCCTGCTGC 
AAAGCCTGCC TGGGGCTTCT TCCTTGCTGC AACCGAGGTC ACATGGTGGG CTTTAAGGAA 
GATCACTATA TGCTTCGGGA GAACCTGATG GCCTCTGACC ACCTGGACAC GCCCATGCTA 
CGAAGCGGGA ACCTCAAGGG ACGAGACACA GTCCGCTGGA AGATCACCAA CAATGTGCAG 
CGCCCTGGCT TTGCCACCCA TGCCGCCAGC ACCAGCCCCA CGGAGCTCGT ACCCTACGGG 
CTGTCCCTGC GCCTTGGCCG CCTCTGCACT GAGAACCTTA TGAAGCCGGG CACCCGAGAG 
TGTGACCAGC TACGCCAGGA GGTGGAGGAA AATCTGAATG AGGTGTATAG ACAGGTCAGC 
GGCGCACACA AGCTCCAGCA GACGAAGTTC CGACAGCAGC CCAACACCGG GAAAAAGCAA 
GACCACACCA TTGTGGACAC AGTGTTGCTG GCGCCCCGCT CCGCCAAGCA GATGCTGCTG 
AAGCTGACAG AGAAGCAGGT GGAGCAGGGG TCCTTCCATG AACTGAAGGT GGCCCCTGGC 
TACTACACTG TCACGGCAGA GCAGGATGCC CGGGGCATGG TGGAGTTCCA GGAGGGCGTG 
GAGCTGGTGG ATGTGCGAGT GCCCCTCTTC ATCCGGCCTG AGGATGATGA TGAGAAGCAG 
CTGCTGGTGG AGGCCATTGA TGTCCCTGTG GGCACTGCCA CCCTTGGTCG CCGTCTGGTA 
AACATCACCA TTATCAAGGA ACAAGCTAGT GGGGTAGTGT CCTTCGAGCA GCCTGAATAC 
TCGGTGAGTC GTGGAGACCA GGTGGCCCGC ATCCCTGTCA TCCGGCACAT CCTGGACAAT 
GGCAAGTCCC AGGTCTCCTA TAGCACACAG GATAATACAG CACACGGACA CCGGGATTAT 
GTCCCTGTGG AGGGAGAGCT GCTGTTCCAT CCTGGGGAGA CCTGGAAGGA GTTGCAGGTG 
AAGCTACTGG AGCTGCAGGA GGTCGACTCC CTCCTGCGTG GCCGCCAGGT CCGCCGCTTC 
CAAGTCCAAC TCAGCAACCC CAAGTTCGGA GCCCGCCTGG GCCAGCCCAG CACAACCACC 
GTTATTCTCG GTGAGCACGA TGAAACGGAC AGGAGTCTCA TAAATCAAAC ACTTTCATCG 
CCTCCGCCAC CCCATGGAGA CCTGGGCGCC CCACAGAACC CCAATGCCAA GGCTGCCGGG 
TCCAGGAAGA TCCATTTCAA CTGGCTGCCC CCTCCTGGCA AGCCAATGGG GTACAGGGTG 
AAGTACTGGA TCCAGGGCGA CTCTGAGTCT GAAGCCCACC TTCTAGACAG CAAGGTGCCC 
TCAGTGGAAC TCACCAACCT GTATCCCTAT TGCGACTACG AAATGAAGGT GTGTGCCTAT 
GGGGCACAGG GTGAGGGGCC CTATAGCTCA CTGGTGTCCT GCCGCACCCA CCAGGAAGTA 
CCCAGCGAGC CAGGGAGGCT GGCTTTCAAT GTAGTCTCTT CTACGGTGAC GCAGCTGAGC 
TGGGCAGAGC CAGCTGAGAC CAATGGCGAG ATCACAGCCT ACGAGGTCTG CTATGGACTG 
GTCAATGAGG ACAACCGACC CATTGGACCT ATGAAGAAGG TGCTCGTGGA CAACCCCAAG 
AACCGGATGC TGCTCATTGA GAATCTGCGA GAATCCCAGC CATACCGATA CACGGTTAAG 
GCGCGCAATG GGGCAGGATG GGGACCCGAG AGAGAGGCTA TCATCAACCT GGCTACACAG 
CCCAAGCGGC CCATGTCCAT CCCTATCATC CCAGACATCC CCATAGTGGA CGCCCAGGGT 
GGAGAAGACT ACGAAAACTT CCTTATGTAC AGTGATGACG TCCTGCGGTC CCCAGCCAGC 
AGCCAGAGGC CCAGCGTTTC TGATGACACT GGCTGCGGCT GGAAGTTCGA GCCTCTGCTC 
GGGGAGGAGC TGGACCTGCG GCGCGTCACG TGGCGGCTAC CCCCGGAACT CATCCCGCGC 
CTGTCCGCCA GCAGCGGGCG CTCTGACGAA GACGGCAGCG TTGCGGGGGG CGTGGAAGGG 
GAGGGCAGCG GCTGGATCCG CGGGGCTACG CCCCGACCCC CGGGAGAGCA CCTGGTGAAT 
GGCCGGATGG ACTTTGCCTA TCCAGGCAGC GCCAACTCCC TGCACAGAAT GACTGCAGCC 
AATGTGGCCT ATGGCACGCA TCTGAGCCCA CACCTGTCCC ACCGAGTGCT GAGCACGTCC 
TCCACCCTTA CCCGGGACTA CCACTCTCTG ACACGCACAG AGCACTCCCA CTCAGGCACA 
CTTCCCAGGG ACTACTCCAC CCTCACTTCC CTTTCCTCCC AAGGCCTCCC TCCTATCTGG 
GAAGATGGGA GGAGCAGGCT TCCGCTGTCC TGGACTCTTG GGTCCTTGAG CCGGGCTCAC 
ATGAAGGGTG TGCCCGCATC CAGGGGTTCA CCAGACTCTA TAATCCTGGC CGGGCAGTCA 
GCAGCACCCT CCTGGGGTAC AGATTCCCGT GGGGCTGTGG GTGTGCCTGA CACACCCACT 
CGGCTGGTGT TCTCTGCCCT GGGGCCCACG TCTTTGAAGG TGAGCTGGCA GGAGCCACAG 
TGTGATCGGA TGCTGCTGGG CTACAGTGTG GAATACCAGC TACTAAACGG CGGCGAGATG 
CATCGGCTCA ACATCCCTAA CCCTGGCCAA ACCTCGGTGG TGGTAGAGGA TCTCCTGCCT 
AACCACTCCT ATGTGTTCCG GGTACGGGCC CAGAGCCAGG AGGGCTGGGG CCGAGAGCGA 
GAGGGTGTCA TCACCATCGA GTCCCAGGTG CACCCGCAGA GCCCTCTCTG CCCCCTGCCA 
GGCTCAGCCT TCACTCTGAG CACCCCCAGC GCCCCGGGAC CGCTGGTGTT CACTGCCCTA 
AGCCCAGACT CCCTGCAGCT CAGCTGGGAG CGGCCGAGGA GGCCCAATGG AGATATCCTT 
GGCTACCTGG TGACCTGTGA GATGGCCCAA GGAGGAGCAC CAGCCAGGAC CTTCCGGGTG 
GACGGAGACA ACCCTGAGAG CCGGTTGACT GTACCTGGCC TCAGTGAGAA CGTTCCTTAC 
AAGTTCAAGG TTCAGGCCAG GACGACCGAG GGCTTCGGGC CAGAGCGTGA GGGTATCATC 
ACCATCGAGT CTCAGGTTGG AGGCCCCTTC CCACAGCTGG GCAGCCATTC TGGGCTCTTC 
CAGAACCCAG TGCAAAGCGA GTTCAGCAGC GTGACCAGCA CGCACAGCAC CACGACTGAG 
CCCTTCCTCA TGGATGGTCT AACCCTGGGG ACCCAGCGCC TGGAAGCAGG AGGCTCCCTC 
ACCCGGCATG TGACCCAGGA ATTCGTGACC CGGACCTTAA CGGCCAGTGG CTCTCTCAGC 
ACTCATATGG ACCAACAGTT CTTCCAAACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169839.1
CDS	436..6048
Translation	MAGPCCSPWVKLLLLAAMLSASLPGDLANRCKKAQVKSCTECIRVDKSCAYCTDELFKERRCNTQAELLAAGCRGESILVMESSLEITENTQIDTSLHRSQVSPQGLQVRLRPGEERSFVFQVFEPLESPVDLYILMDFSNSMSDDLDNLKQMGQNLAKILRQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTENVEEFWNKLQGERISGNLDAPEGGFDAILQTAVCTRDIGWRADSTHLLVFSTESAFHYEADGANVLAGIMNRNDEKCHLDASGAYTQYKTQDYPSVPTLVRLLAKHNIIPIFAVTNYSYSYYEKLHKYFPVSSLGVLQEDSSNIVELLEEAFYRIRSNLDIRALDSPRGLRTEVTSDTLQKTETGSFHIKRGEVGTYNVHLRAVEDIDGTHVCQLAKEDQGGNIHLKPSFSDGLRMDASVICDVCPCELQKEVRSARCHFRGDFMCGHCVCNEGWSGKTCNCSTGSLSDTQPCLREGEDKPCSGHGECQCGRCVCYGEGRYEGHFCEYDNFQCPRTSGFLCNDRGRCSMGECVCEPGWTGRSCDCPLSNATCIDSNGGICNGRGYCECGRCHCNQQSLYTDTTCEINYSAIRLGLCEDLRSCVQCQAWGTGEKKGRACDDCPFKVKMVDELKKEEVVEYCSFRDEDDDCTYSYNVEGDGSPGPNSTVLVHKKKDCPPGSFWWLIPLLIFLLLLLALLLLLCWKYCACCKACLGLLPCCNRGHMVGFKEDHYMLRENLMASDHLDTPMLRSGNLKGRDTVRWKITNNVQRPGFATHAASTSPTELVPYGLSLRLGRLCTENLMKPGTRECDQLRQEVEENLNEVYRQVSGAHKLQQTKFRQQPNTGKKQDHTIVDTVLLAPRSAKQMLLKLTEKQVEQGSFHELKVAPGYYTVTAEQDARGMVEFQEGVELVDVRVPLFIRPEDDDEKQLLVEAIDVPVGTATLGRRLVNITIIKEQASGVVSFEQPEYSVSRGDQVARIPVIRHILDNGKSQVSYSTQDNTAHGHRDYVPVEGELLFHPGETWKELQVKLLELQEVDSLLRGRQVRRFQVQLSNPKFGARLGQPSTTTVILGEHDETDRSLINQTLSSPPPPHGDLGAPQNPNAKAAGSRKIHFNWLPPPGKPMGYRVKYWIQGDSESEAHLLDSKVPSVELTNLYPYCDYEMKVCAYGAQGEGPYSSLVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYGLVNEDNRPIGPMKKVLVDNPKNRMLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDIPIVDAQGGEDYENFLMYSDDVLRSPASSQRPSVSDDTGCGWKFEPLLGEELDLRRVTWRLPPELIPRLSASSGRSDEDGSVAGGVEGEGSGWIRGATPRPPGEHLVNGRMDFAYPGSANSLHRMTAANVAYGTHLSPHLSHRVLSTSSTLTRDYHSLTRTEHSHSGTLPRDYSTLTSLSSQGLPPIWEDGRSRLPLSWTLGSLSRAHMKGVPASRGSPDSIILAGQSAAPSWGTDSRGAVGVPDTPTRLVFSALGPTSLKVSWQEPQCDRMLLGYSVEYQLLNGGEMHRLNIPNPGQTSVVVEDLLPNHSYVFRVRAQSQEGWGREREGVITIESQVHPQSPLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPNGDILGYLVTCEMAQGGAPARTFRVDGDNPESRLTVPGLSENVPYKFKVQARTTEGFGPEREGIITIESQVGGPFPQLGSHSGLFQNPVQSEFSSVTSTHSTTTEPFLMDGLTLGTQRLEAGGSLTRHVTQEFVTRTLTASGSLSTHMDQQFFQT

Target ORF information:

RefSeq Version	XM_017314350.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus integrin beta 4 (Itgb4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314350.2

ORF Insert Sequence:

ATGGCAGGGC CCTGTTGCAG CCCATGGGTG AAGCTGCTGC TGCTGGCAGC AATGCTGAGT 
GCCAGCCTCC CTGGAGACCT GGCCAACCGC TGCAAGAAGG CTCAGGTGAA GAGCTGTACC 
GAGTGCATCC GGGTGGACAA GAGCTGTGCC TACTGCACAG ACGAGCTGTT CAAGGAGAGG 
CGCTGCAACA CCCAGGCGGA GCTTCTGGCT GCAGGCTGCA GGGGAGAGAG CATCCTGGTC 
ATGGAGAGCA GCCTTGAAAT CACAGAGAAC ACCCAGATCG ACACCAGCCT GCACCGCAGC 
CAGGTATCTC CCCAAGGCCT GCAAGTCCGG CTGCGGCCGG GTGAGGAGCG CAGCTTTGTG 
TTCCAGGTGT TTGAGCCCCT GGAGAGCCCC GTGGATCTGT ATATCCTCAT GGACTTCTCC 
AACTCCATGT CTGACGATCT GGACAACCTC AAGCAGATGG GGCAGAACCT GGCCAAGATC 
CTGCGCCAGC TCACCAGCGA CTACACCATT GGATTTGGAA AGTTTGTGGA CAAAGTCAGC 
GTCCCACAGA CAGACATGAG GCCCGAGAAA CTGAAGGAGC CCTGGCCCAA CAGTGATCCC 
CCGTTCTCCT TCAAGAACGT TATCAGCTTA ACGGAGAATG TGGAAGAATT CTGGAACAAA 
CTGCAAGGAG AACGCATCTC AGGCAACCTG GACGCTCCTG AAGGGGGCTT TGATGCCATC 
CTGCAGACAG CTGTGTGCAC AAGGGACATT GGCTGGAGGG CTGACAGCAC CCACCTGCTG 
GTGTTCTCCA CCGAGTCTGC CTTCCACTAC GAGGCTGATG GTGCCAACGT GCTGGCCGGC 
ATCATGAACC GCAATGATGA GAAATGCCAC CTGGACGCCT CGGGCGCCTA CACCCAATAC 
AAGACACAGG ACTACCCATC AGTGCCCACG CTGGTTCGCC TGCTTGCCAA GCATAACATC 
ATCCCCATCT TTGCTGTCAC CAACTACTCT TACAGCTACT ATGAGAAGCT CCATAAGTAT 
TTCCCCGTCT CCTCTCTGGG CGTCCTGCAG GAGGATTCAT CCAACATCGT GGAGCTGCTG 
GAGGAGGCCT TCTATCGAAT TCGCTCCAAC CTGGACATCC GGGCTCTGGA CAGCCCCAGA 
GGCCTGAGAA CAGAGGTCAC CTCCGATACT CTCCAGAAGA CGGAGACTGG GTCCTTTCAC 
ATCAAGCGGG GGGAAGTGGG CACATACAAT GTGCATCTCC GGGCAGTGGA GGACATAGAT 
GGGACACATG TGTGCCAGCT GGCTAAAGAA GACCAAGGGG GCAACATCCA CCTGAAACCC 
TCCTTCTCTG ATGGCCTCCG GATGGACGCG AGTGTGATCT GTGACGTGTG CCCCTGTGAG 
CTGCAAAAGG AAGTTCGATC AGCTCGCTGT CACTTCAGAG GAGACTTCAT GTGTGGACAC 
TGTGTGTGCA ATGAGGGCTG GAGTGGCAAA ACCTGCAACT GCTCCACCGG CTCTCTGAGT 
GACACACAGC CCTGCCTGCG TGAGGGTGAG GACAAACCGT GCTCGGGCCA CGGCGAGTGC 
CAGTGCGGAC GCTGTGTGTG CTATGGTGAA GGCCGCTACG AGGGTCACTT CTGCGAGTAT 
GACAACTTCC AGTGTCCCCG GACCTCTGGA TTCCTGTGCA ATGACCGGGG TCGCTGTTCT 
ATGGGAGAGT GTGTGTGTGA GCCTGGTTGG ACAGGCCGCA GCTGTGACTG TCCCCTCAGC 
AATGCCACCT GCATCGATAG CAACGGGGGC ATCTGCAACG GCCGAGGCTA CTGTGAGTGT 
GGCCGTTGTC ACTGCAACCA GCAGTCGCTC TACACGGACA CCACCTGTGA GATCAACTAC 
TCTGCGATAC GCCTGGGTCT CTGTGAGGAT CTCCGCTCCT GCGTACAGTG CCAGGCCTGG 
GGCACCGGGG AGAAGAAAGG GCGCGCGTGT GACGATTGCC CCTTTAAAGT CAAGATGGTA 
GACGAGCTTA AGAAAGAAGA GGTGGTGGAG TACTGCTCCT TCCGGGATGA GGATGACGAC 
TGCACTTACA GCTACAACGT GGAGGGCGAT GGCAGCCCTG GGCCCAACAG CACAGTCCTG 
GTCCACAAAA AGAAAGACTG CCCTCCCGGC TCCTTCTGGT GGCTCATCCC CCTGCTCATC 
TTCCTCCTGT TGCTCCTGGC GTTGCTTCTG CTGCTCTGCT GGAAATACTG TGCCTGCTGC 
AAAGCCTGCC TGGGGCTTCT TCCTTGCTGC AACCGAGGTC ACATGGTGGG CTTTAAGGAA 
GATCACTATA TGCTTCGGGA GAACCTGATG GCCTCTGACC ACCTGGACAC GCCCATGCTA 
CGAAGCGGGA ACCTCAAGGG ACGAGACACA GTCCGCTGGA AGATCACCAA CAATGTGCAG 
CGCCCTGGCT TTGCCACCCA TGCCGCCAGC ACCAGCCCCA CGGAGCTCGT ACCCTACGGG 
CTGTCCCTGC GCCTTGGCCG CCTCTGCACT GAGAACCTTA TGAAGCCGGG CACCCGAGAG 
TGTGACCAGC TACGCCAGGA GGTGGAGGAA AATCTGAATG AGGTGTATAG ACAGGTCAGC 
GGCGCACACA AGCTCCAGCA GACGAAGTTC CGACAGCAGC CCAACACCGG GAAAAAGCAA 
GACCACACCA TTGTGGACAC AGTGTTGCTG GCGCCCCGCT CCGCCAAGCA GATGCTGCTG 
AAGCTGACAG AGAAGCAGGT GGAGCAGGGG TCCTTCCATG AACTGAAGGT GGCCCCTGGC 
TACTACACTG TCACGGCAGA GCAGGATGCC CGGGGCATGG TGGAGTTCCA GGAGGGCGTG 
GAGCTGGTGG ATGTGCGAGT GCCCCTCTTC ATCCGGCCTG AGGATGATGA TGAGAAGCAG 
CTGCTGGTGG AGGCCATTGA TGTCCCTGTG GGCACTGCCA CCCTTGGTCG CCGTCTGGTA 
AACATCACCA TTATCAAGGA ACAAGCTAGT GGGGTAGTGT CCTTCGAGCA GCCTGAATAC 
TCGGTGAGTC GTGGAGACCA GGTGGCCCGC ATCCCTGTCA TCCGGCACAT CCTGGACAAT 
GGCAAGTCCC AGGTCTCCTA TAGCACACAG GATAATACAG CACACGGACA CCGGGATTAT 
GTCCCTGTGG AGGGAGAGCT GCTGTTCCAT CCTGGGGAGA CCTGGAAGGA GTTGCAGGTG 
AAGCTACTGG AGCTGCAGGA GGTCGACTCC CTCCTGCGTG GCCGCCAGGT CCGCCGCTTC 
CAAGTCCAAC TCAGCAACCC CAAGTTCGGA GCCCGCCTGG GCCAGCCCAG CACAACCACC 
GTTATTCTCG GTGAGCACGA TGAAACGGAC AGGAGTCTCA TAAATCAAAC ACTTTCATCG 
CCTCCGCCAC CCCATGGAGA CCTGGGCGCC CCACAGAACC CCAATGCCAA GGCTGCCGGG 
TCCAGGAAGA TCCATTTCAA CTGGCTGCCC CCTCCTGGCA AGCCAATGGG GTACAGGGTG 
AAGTACTGGA TCCAGGGCGA CTCTGAGTCT GAAGCCCACC TTCTAGACAG CAAGGTGCCC 
TCAGTGGAAC TCACCAACCT GTATCCCTAT TGCGACTACG AAATGAAGGT GTGTGCCTAT 
GGGGCACAGG GTGAGGGGCC CTATAGCTCA CTGGTGTCCT GCCGCACCCA CCAGGAAGTA 
CCCAGCGAGC CAGGGAGGCT GGCTTTCAAT GTAGTCTCTT CTACGGTGAC GCAGCTGAGC 
TGGGCAGAGC CAGCTGAGAC CAATGGCGAG ATCACAGCCT ACGAGGTCTG CTATGGACTG 
GTCAATGAGG ACAACCGACC CATTGGACCT ATGAAGAAGG TGCTCGTGGA CAACCCCAAG 
AACCGGATGC TGCTCATTGA GAATCTGCGA GAATCCCAGC CATACCGATA CACGGTTAAG 
GCGCGCAATG GGGCAGGATG GGGACCCGAG AGAGAGGCTA TCATCAACCT GGCTACACAG 
CCCAAGCGGC CCATGTCCAT CCCTATCATC CCAGACATCC CCATAGTGGA CGCCCAGGGT 
GGAGAAGACT ACGAAAACTT CCTTATGTAC AGTGATGACG TCCTGCGGTC CCCAGCCAGC 
AGCCAGAGGC CCAGCGTTTC TGATGACACT GGCTGCGGCT GGAAGTTCGA GCCTCTGCTC 
GGGGAGGAGC TGGACCTGCG GCGCGTCACG TGGCGGCTAC CCCCGGAACT CATCCCGCGC 
CTGTCCGCCA GCAGCGGGCG CTCTGACGAA GACGGCAGCG TTGCGGGGGG CGTGGAAGGG 
GAGGGCAGCG GCTGGATCCG CGGGGCTACG CCCCGACCCC CGGGAGAGCA CCTGGTGAAT 
GGCCGGATGG ACTTTGCCTA TCCAGGCAGC GCCAACTCCC TGCACAGAAT GACTGCAGCC 
AATGTGGCCT ATGGCACGCA TCTGAGCCCA CACCTGTCCC ACCGAGTGCT GAGCACGTCC 
TCCACCCTTA CCCGGGACTA CCACTCTCTG ACACGCACAG AGCACTCCCA CTCAGGCACA 
CTTCCCAGGG ACTACTCCAC CCTCACTTCC CTTTCCTCCC AAGGCCTCCC TCCTATCTGG 
GAAGATGGGA GGAGCAGGCT TCCGCTGTCC TGGACTCTTG GGTCCTTGAG CCGGGCTCAC 
ATGAAGGGTG TGCCCGCATC CAGGGGTTCA CCAGACTCTA TAATCCTGGC CGGGCAGTCA 
GCAGCACCCT CCTGGGGTAC AGATTCCCGT GGGGCTGTGG GTGTGCCTGA CACACCCACT 
CGGCTGGTGT TCTCTGCCCT GGGGCCCACG TCTTTGAAGG TGAGCTGGCA GGAGCCACAG 
TGTGATCGGA TGCTGCTGGG CTACAGTGTG GAATACCAGC TACTAAACGG CGGCGAGATG 
CATCGGCTCA ACATCCCTAA CCCTGGCCAA ACCTCGGTGG TGGTAGAGGA TCTCCTGCCT 
AACCACTCCT ATGTGTTCCG GGTACGGGCC CAGAGCCAGG AGGGCTGGGG CCGAGAGCGA 
GAGGGTGTCA TCACCATCGA GTCCCAGGTG CACCCGCAGA GCCCTCTCTG CCCCCTGCCA 
GGCTCAGCCT TCACTCTGAG CACCCCCAGC GCCCCGGGAC CGCTGGTGTT CACTGCCCTA 
AGCCCAGACT CCCTGCAGCT CAGCTGGGAG CGGCCGAGGA GGCCCAATGG AGATATCCTT 
GGCTACCTGG TGACCTGTGA GATGGCCCAA GGAGGAGCAC CAGCCAGGAC CTTCCGGGTG 
GACGGAGACA ACCCTGAGAG CCGGTTGACT GTACCTGGCC TCAGTGAGAA CGTTCCTTAC 
AAGTTCAAGG TTCAGGCCAG GACGACCGAG GGCTTCGGGC CAGAGCGTGA GGGTATCATC 
ACCATCGAGT CTCAGGTTGG AGGCCCCTTC CCACAGCTGG GCAGCCATTC TGGGCTCTTC 
CAGAACCCAG TGCAAAGCGA GTTCAGCAGC GTGACCAGCA CGCACAGCAC CACGACTGAG 
CCCTTCCTCA TGGATGGTCT AACCCTGGGG ACCCAGCGCC TGGAAGCAGG AGGCTCCCTC 
ACCCGGCATG TGACCCAGGA ATTCGTGACC CGGACCTTAA CGGCCAGTGG CTCTCTCAGC 
ACTCATATGG ACCAACAGTT CTTCCAAACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itgb4 ( XM_017314351.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169840.1 Mus musculus integrin beta 4 (Itgb4), transcript variant X3, mRNA.

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CloneID	OMu77783
Clone ID Related Accession (Same CDS sequence)	XM_017314351.1 , XM_017314351.2
Accession Version	XM_017314351.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	integrin beta-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGC CCTGTTGCAG CCCATGGGTG AAGCTGCTGC TGCTGGCAGC AATGCTGAGT 
GCCAGCCTCC CTGGAGACCT GGCCAACCGC TGCAAGAAGG CTCAGGTGAA GAGCTGTACC 
GAGTGCATCC GGGTGGACAA GAGCTGTGCC TACTGCACAG ACGAGCTGTT CAAGGAGAGG 
CGCTGCAACA CCCAGGCGGA GCTTCTGGCT GCAGGCTGCA GGGGAGAGAG CATCCTGGTC 
ATGGAGAGCA GCCTTGAAAT CACAGAGAAC ACCCAGATCG ACACCAGCCT GCACCGCAGC 
CAGGTATCTC CCCAAGGCCT GCAAGTCCGG CTGCGGCCGG GTGAGGAGCG CAGCTTTGTG 
TTCCAGGTGT TTGAGCCCCT GGAGAGCCCC GTGGATCTGT ATATCCTCAT GGACTTCTCC 
AACTCCATGT CTGACGATCT GGACAACCTC AAGCAGATGG GGCAGAACCT GGCCAAGATC 
CTGCGCCAGC TCACCAGCGA CTACACCATT GGATTTGGAA AGTTTGTGGA CAAAGTCAGC 
GTCCCACAGA CAGACATGAG GCCCGAGAAA CTGAAGGAGC CCTGGCCCAA CAGTGATCCC 
CCGTTCTCCT TCAAGAACGT TATCAGCTTA ACGGAGAATG TGGAAGAATT CTGGAACAAA 
CTGCAAGGAG AACGCATCTC AGGCAACCTG GACGCTCCTG AAGGGGGCTT TGATGCCATC 
CTGCAGACAG CTGTGTGCAC AAGGGACATT GGCTGGAGGG CTGACAGCAC CCACCTGCTG 
GTGTTCTCCA CCGAGTCTGC CTTCCACTAC GAGGCTGATG GTGCCAACGT GCTGGCCGGC 
ATCATGAACC GCAATGATGA GAAATGCCAC CTGGACGCCT CGGGCGCCTA CACCCAATAC 
AAGACACAGG ACTACCCATC AGTGCCCACG CTGGTTCGCC TGCTTGCCAA GCATAACATC 
ATCCCCATCT TTGCTGTCAC CAACTACTCT TACAGCTACT ATGAGAAGCT CCATAAGTAT 
TTCCCCGTCT CCTCTCTGGG CGTCCTGCAG GAGGATTCAT CCAACATCGT GGAGCTGCTG 
GAGGAGGCCT TCTATCGAAT TCGCTCCAAC CTGGACATCC GGGCTCTGGA CAGCCCCAGA 
GGCCTGAGAA CAGAGGTCAC CTCCGATACT CTCCAGAAGA CGGAGACTGG GTCCTTTCAC 
ATCAAGCGGG GGGAAGTGGG CACATACAAT GTGCATCTCC GGGCAGTGGA GGACATAGAT 
GGGACACATG TGTGCCAGCT GGCTAAAGAA GACCAAGGGG GCAACATCCA CCTGAAACCC 
TCCTTCTCTG ATGGCCTCCG GATGGACGCG AGTGTGATCT GTGACGTGTG CCCCTGTGAG 
CTGCAAAAGG AAGTTCGATC AGCTCGCTGT CACTTCAGAG GAGACTTCAT GTGTGGACAC 
TGTGTGTGCA ATGAGGGCTG GAGTGGCAAA ACCTGCAACT GCTCCACCGG CTCTCTGAGT 
GACACACAGC CCTGCCTGCG TGAGGGTGAG GACAAACCGT GCTCGGGCCA CGGCGAGTGC 
CAGTGCGGAC GCTGTGTGTG CTATGGTGAA GGCCGCTACG AGGGTCACTT CTGCGAGTAT 
GACAACTTCC AGTGTCCCCG GACCTCTGGA TTCCTGTGCA ATGACCGGGG TCGCTGTTCT 
ATGGGAGAGT GTGTGTGTGA GCCTGGTTGG ACAGGCCGCA GCTGTGACTG TCCCCTCAGC 
AATGCCACCT GCATCGATAG CAACGGGGGC ATCTGCAACG GCCGAGGCTA CTGTGAGTGT 
GGCCGTTGTC ACTGCAACCA GCAGTCGCTC TACACGGACA CCACCTGTGA GATCAACTAC 
TCTGCGATAC GCCTGGGTCT CTGTGAGGAT CTCCGCTCCT GCGTACAGTG CCAGGCCTGG 
GGCACCGGGG AGAAGAAAGG GCGCGCGTGT GACGATTGCC CCTTTAAAGT CAAGATGGTA 
GACGAGCTTA AGAAAGCAGA AGAGGTGGTG GAGTACTGCT CCTTCCGGGA TGAGGATGAC 
GACTGCACTT ACAGCTACAA CGTGGAGGGC GATGGCAGCC CTGGGCCCAA CAGCACAGTC 
CTGGTCCACA AAAAGAAAGA CTGCCCTCCC GGCTCCTTCT GGTGGCTCAT CCCCCTGCTC 
ATCTTCCTCC TGTTGCTCCT GGCGTTGCTT CTGCTGCTCT GCTGGAAATA CTGTGCCTGC 
TGCAAAGCCT GCCTGGGGCT TCTTCCTTGC TGCAACCGAG GTCACATGGT GGGCTTTAAG 
GAAGATCACT ATATGCTTCG GGAGAACCTG ATGGCCTCTG ACCACCTGGA CACGCCCATG 
CTACGAAGCG GGAACCTCAA GGGACGAGAC ACAGTCCGCT GGAAGATCAC CAACAATGTG 
CAGCGCCCTG GCTTTGCCAC CCATGCCGCC AGCACCAGCC CCACGGAGCT CGTACCCTAC 
GGGCTGTCCC TGCGCCTTGG CCGCCTCTGC ACTGAGAACC TTATGAAGCC GGGCACCCGA 
GAGTGTGACC AGCTACGCCA GGAGGTGGAG GAAAATCTGA ATGAGGTGTA TAGACAGGTC 
AGCGGCGCAC ACAAGCTCCA GCAGACGAAG TTCCGACAGC AGCCCAACAC CGGGAAAAAG 
CAAGACCACA CCATTGTGGA CACAGTGTTG CTGGCGCCCC GCTCCGCCAA GCAGATGCTG 
CTGAAGCTGA CAGAGAAGCA GGTGGAGCAG GGGTCCTTCC ATGAACTGAA GGTGGCCCCT 
GGCTACTACA CTGTCACGGC AGAGCAGGAT GCCCGGGGCA TGGTGGAGTT CCAGGAGGGC 
GTGGAGCTGG TGGATGTGCG AGTGCCCCTC TTCATCCGGC CTGAGGATGA TGATGAGAAG 
CAGCTGCTGG TGGAGGCCAT TGATGTCCCT GTGGGCACTG CCACCCTTGG TCGCCGTCTG 
GTAAACATCA CCATTATCAA GGAACAAGCT AGTGGGGTAG TGTCCTTCGA GCAGCCTGAA 
TACTCGGTGA GTCGTGGAGA CCAGGTGGCC CGCATCCCTG TCATCCGGCA CATCCTGGAC 
AATGGCAAGT CCCAGGTCTC CTATAGCACA CAGGATAATA CAGCACACGG ACACCGGGAT 
TATGTCCCTG TGGAGGGAGA GCTGCTGTTC CATCCTGGGG AGACCTGGAA GGAGTTGCAG 
GTGAAGCTAC TGGAGCTGCA GGAGGTCGAC TCCCTCCTGC GTGGCCGCCA GGTCCGCCGC 
TTCCAAGTCC AACTCAGCAA CCCCAAGTTC GGAGCCCGCC TGGGCCAGCC CAGCACAACC 
ACCGTTATTC TCGATGAAAC GGACAGGAGT CTCATAAATC AAACACTTTC ATCGCCTCCG 
CCACCCCATG GAGACCTGGG CGCCCCACAG AACCCCAATG CCAAGGCTGC CGGGTCCAGG 
AAGATCCATT TCAACTGGCT GCCCCCTCCT GGCAAGCCAA TGGGGTACAG GGTGAAGTAC 
TGGATCCAGG GCGACTCTGA GTCTGAAGCC CACCTTCTAG ACAGCAAGGT GCCCTCAGTG 
GAACTCACCA ACCTGTATCC CTATTGCGAC TACGAAATGA AGGTGTGTGC CTATGGGGCA 
CAGGGTGAGG GGCCCTATAG CTCACTGGTG TCCTGCCGCA CCCACCAGGA AGTACCCAGC 
GAGCCAGGGA GGCTGGCTTT CAATGTAGTC TCTTCTACGG TGACGCAGCT GAGCTGGGCA 
GAGCCAGCTG AGACCAATGG CGAGATCACA GCCTACGAGG TCTGCTATGG ACTGGTCAAT 
GAGGACAACC GACCCATTGG ACCTATGAAG AAGGTGCTCG TGGACAACCC CAAGAACCGG 
ATGCTGCTCA TTGAGAATCT GCGAGAATCC CAGCCATACC GATACACGGT TAAGGCGCGC 
AATGGGGCAG GATGGGGACC CGAGAGAGAG GCTATCATCA ACCTGGCTAC ACAGCCCAAG 
CGGCCCATGT CCATCCCTAT CATCCCAGAC ATCCCCATAG TGGACGCCCA GGGTGGAGAA 
GACTACGAAA ACTTCCTTAT GTACAGTGAT GACGTCCTGC GGTCCCCAGC CAGCAGCCAG 
AGGCCCAGCG TTTCTGATGA CACTGGCTGC GGCTGGAAGT TCGAGCCTCT GCTCGGGGAG 
GAGCTGGACC TGCGGCGCGT CACGTGGCGG CTACCCCCGG AACTCATCCC GCGCCTGTCC 
GCCAGCAGCG GGCGCTCTGA CGAAGACGGC AGCGTTGCGG GGGGCGTGGA AGGGGAGGGC 
AGCGGCTGGA TCCGCGGGGC TACGCCCCGA CCCCCGGGAG AGCACCTGGT GAATGGCCGG 
ATGGACTTTG CCTATCCAGG CAGCGCCAAC TCCCTGCACA GAATGACTGC AGCCAATGTG 
GCCTATGGCA CGCATCTGAG CCCACACCTG TCCCACCGAG TGCTGAGCAC GTCCTCCACC 
CTTACCCGGG ACTACCACTC TCTGACACGC ACAGAGCACT CCCACTCAGG CACACTTCCC 
AGGGACTACT CCACCCTCAC TTCCCTTTCC TCCCAAGGCC TCCCTCCTAT CTGGGAAGAT 
GGGAGGAGCA GGCTTCCGCT GTCCTGGACT CTTGGGTCCT TGAGCCGGGC TCACATGAAG 
GGTGTGCCCG CATCCAGGGG TTCACCAGAC TCTATAATCC TGGCCGGGCA GTCAGCAGCA 
CCCTCCTGGG GTACAGATTC CCGTGGGGCT GTGGGTGTGC CTGACACACC CACTCGGCTG 
GTGTTCTCTG CCCTGGGGCC CACGTCTTTG AAGGTGAGCT GGCAGGAGCC ACAGTGTGAT 
CGGATGCTGC TGGGCTACAG TGTGGAATAC CAGCTACTAA ACGGCGGCGA GATGCATCGG 
CTCAACATCC CTAACCCTGG CCAAACCTCG GTGGTGGTAG AGGATCTCCT GCCTAACCAC 
TCCTATGTGT TCCGGGTACG GGCCCAGAGC CAGGAGGGCT GGGGCCGAGA GCGAGAGGGT 
GTCATCACCA TCGAGTCCCA GGTGCACCCG CAGAGCCCTC TCTGCCCCCT GCCAGGCTCA 
GCCTTCACTC TGAGCACCCC CAGCGCCCCG GGACCGCTGG TGTTCACTGC CCTAAGCCCA 
GACTCCCTGC AGCTCAGCTG GGAGCGGCCG AGGAGGCCCA ATGGAGATAT CCTTGGCTAC 
CTGGTGACCT GTGAGATGGC CCAAGGAGGA GCACCAGCCA GGACCTTCCG GGTGGACGGA 
GACAACCCTG AGAGCCGGTT GACTGTACCT GGCCTCAGTG AGAACGTTCC TTACAAGTTC 
AAGGTTCAGG CCAGGACGAC CGAGGGCTTC GGGCCAGAGC GTGAGGGTAT CATCACCATC 
GAGTCTCAGG TTGGAGGCCC CTTCCCACAG CTGGGCAGCC ATTCTGGGCT CTTCCAGAAC 
CCAGTGCAAA GCGAGTTCAG CAGCGTGACC AGCACGCACA GCACCACGAC TGAGCCCTTC 
CTCATGGATG GTCTAACCCT GGGGACCCAG CGCCTGGAAG CAGGAGGCTC CCTCACCCGG 
CATGTGACCC AGGAATTCGT GACCCGGACC TTAACGGCCA GTGGCTCTCT CAGCACTCAT 
ATGGACCAAC AGTTCTTCCA AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169840.1
CDS	436..6042
Translation	MAGPCCSPWVKLLLLAAMLSASLPGDLANRCKKAQVKSCTECIRVDKSCAYCTDELFKERRCNTQAELLAAGCRGESILVMESSLEITENTQIDTSLHRSQVSPQGLQVRLRPGEERSFVFQVFEPLESPVDLYILMDFSNSMSDDLDNLKQMGQNLAKILRQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTENVEEFWNKLQGERISGNLDAPEGGFDAILQTAVCTRDIGWRADSTHLLVFSTESAFHYEADGANVLAGIMNRNDEKCHLDASGAYTQYKTQDYPSVPTLVRLLAKHNIIPIFAVTNYSYSYYEKLHKYFPVSSLGVLQEDSSNIVELLEEAFYRIRSNLDIRALDSPRGLRTEVTSDTLQKTETGSFHIKRGEVGTYNVHLRAVEDIDGTHVCQLAKEDQGGNIHLKPSFSDGLRMDASVICDVCPCELQKEVRSARCHFRGDFMCGHCVCNEGWSGKTCNCSTGSLSDTQPCLREGEDKPCSGHGECQCGRCVCYGEGRYEGHFCEYDNFQCPRTSGFLCNDRGRCSMGECVCEPGWTGRSCDCPLSNATCIDSNGGICNGRGYCECGRCHCNQQSLYTDTTCEINYSAIRLGLCEDLRSCVQCQAWGTGEKKGRACDDCPFKVKMVDELKKAEEVVEYCSFRDEDDDCTYSYNVEGDGSPGPNSTVLVHKKKDCPPGSFWWLIPLLIFLLLLLALLLLLCWKYCACCKACLGLLPCCNRGHMVGFKEDHYMLRENLMASDHLDTPMLRSGNLKGRDTVRWKITNNVQRPGFATHAASTSPTELVPYGLSLRLGRLCTENLMKPGTRECDQLRQEVEENLNEVYRQVSGAHKLQQTKFRQQPNTGKKQDHTIVDTVLLAPRSAKQMLLKLTEKQVEQGSFHELKVAPGYYTVTAEQDARGMVEFQEGVELVDVRVPLFIRPEDDDEKQLLVEAIDVPVGTATLGRRLVNITIIKEQASGVVSFEQPEYSVSRGDQVARIPVIRHILDNGKSQVSYSTQDNTAHGHRDYVPVEGELLFHPGETWKELQVKLLELQEVDSLLRGRQVRRFQVQLSNPKFGARLGQPSTTTVILDETDRSLINQTLSSPPPPHGDLGAPQNPNAKAAGSRKIHFNWLPPPGKPMGYRVKYWIQGDSESEAHLLDSKVPSVELTNLYPYCDYEMKVCAYGAQGEGPYSSLVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYGLVNEDNRPIGPMKKVLVDNPKNRMLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDIPIVDAQGGEDYENFLMYSDDVLRSPASSQRPSVSDDTGCGWKFEPLLGEELDLRRVTWRLPPELIPRLSASSGRSDEDGSVAGGVEGEGSGWIRGATPRPPGEHLVNGRMDFAYPGSANSLHRMTAANVAYGTHLSPHLSHRVLSTSSTLTRDYHSLTRTEHSHSGTLPRDYSTLTSLSSQGLPPIWEDGRSRLPLSWTLGSLSRAHMKGVPASRGSPDSIILAGQSAAPSWGTDSRGAVGVPDTPTRLVFSALGPTSLKVSWQEPQCDRMLLGYSVEYQLLNGGEMHRLNIPNPGQTSVVVEDLLPNHSYVFRVRAQSQEGWGREREGVITIESQVHPQSPLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPNGDILGYLVTCEMAQGGAPARTFRVDGDNPESRLTVPGLSENVPYKFKVQARTTEGFGPEREGIITIESQVGGPFPQLGSHSGLFQNPVQSEFSSVTSTHSTTTEPFLMDGLTLGTQRLEAGGSLTRHVTQEFVTRTLTASGSLSTHMDQQFFQT

Target ORF information:

RefSeq Version	XM_017314351.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus integrin beta 4 (Itgb4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314351.1

ORF Insert Sequence:

ATGGCAGGGC CCTGTTGCAG CCCATGGGTG AAGCTGCTGC TGCTGGCAGC AATGCTGAGT 
GCCAGCCTCC CTGGAGACCT GGCCAACCGC TGCAAGAAGG CTCAGGTGAA GAGCTGTACC 
GAGTGCATCC GGGTGGACAA GAGCTGTGCC TACTGCACAG ACGAGCTGTT CAAGGAGAGG 
CGCTGCAACA CCCAGGCGGA GCTTCTGGCT GCAGGCTGCA GGGGAGAGAG CATCCTGGTC 
ATGGAGAGCA GCCTTGAAAT CACAGAGAAC ACCCAGATCG ACACCAGCCT GCACCGCAGC 
CAGGTATCTC CCCAAGGCCT GCAAGTCCGG CTGCGGCCGG GTGAGGAGCG CAGCTTTGTG 
TTCCAGGTGT TTGAGCCCCT GGAGAGCCCC GTGGATCTGT ATATCCTCAT GGACTTCTCC 
AACTCCATGT CTGACGATCT GGACAACCTC AAGCAGATGG GGCAGAACCT GGCCAAGATC 
CTGCGCCAGC TCACCAGCGA CTACACCATT GGATTTGGAA AGTTTGTGGA CAAAGTCAGC 
GTCCCACAGA CAGACATGAG GCCCGAGAAA CTGAAGGAGC CCTGGCCCAA CAGTGATCCC 
CCGTTCTCCT TCAAGAACGT TATCAGCTTA ACGGAGAATG TGGAAGAATT CTGGAACAAA 
CTGCAAGGAG AACGCATCTC AGGCAACCTG GACGCTCCTG AAGGGGGCTT TGATGCCATC 
CTGCAGACAG CTGTGTGCAC AAGGGACATT GGCTGGAGGG CTGACAGCAC CCACCTGCTG 
GTGTTCTCCA CCGAGTCTGC CTTCCACTAC GAGGCTGATG GTGCCAACGT GCTGGCCGGC 
ATCATGAACC GCAATGATGA GAAATGCCAC CTGGACGCCT CGGGCGCCTA CACCCAATAC 
AAGACACAGG ACTACCCATC AGTGCCCACG CTGGTTCGCC TGCTTGCCAA GCATAACATC 
ATCCCCATCT TTGCTGTCAC CAACTACTCT TACAGCTACT ATGAGAAGCT CCATAAGTAT 
TTCCCCGTCT CCTCTCTGGG CGTCCTGCAG GAGGATTCAT CCAACATCGT GGAGCTGCTG 
GAGGAGGCCT TCTATCGAAT TCGCTCCAAC CTGGACATCC GGGCTCTGGA CAGCCCCAGA 
GGCCTGAGAA CAGAGGTCAC CTCCGATACT CTCCAGAAGA CGGAGACTGG GTCCTTTCAC 
ATCAAGCGGG GGGAAGTGGG CACATACAAT GTGCATCTCC GGGCAGTGGA GGACATAGAT 
GGGACACATG TGTGCCAGCT GGCTAAAGAA GACCAAGGGG GCAACATCCA CCTGAAACCC 
TCCTTCTCTG ATGGCCTCCG GATGGACGCG AGTGTGATCT GTGACGTGTG CCCCTGTGAG 
CTGCAAAAGG AAGTTCGATC AGCTCGCTGT CACTTCAGAG GAGACTTCAT GTGTGGACAC 
TGTGTGTGCA ATGAGGGCTG GAGTGGCAAA ACCTGCAACT GCTCCACCGG CTCTCTGAGT 
GACACACAGC CCTGCCTGCG TGAGGGTGAG GACAAACCGT GCTCGGGCCA CGGCGAGTGC 
CAGTGCGGAC GCTGTGTGTG CTATGGTGAA GGCCGCTACG AGGGTCACTT CTGCGAGTAT 
GACAACTTCC AGTGTCCCCG GACCTCTGGA TTCCTGTGCA ATGACCGGGG TCGCTGTTCT 
ATGGGAGAGT GTGTGTGTGA GCCTGGTTGG ACAGGCCGCA GCTGTGACTG TCCCCTCAGC 
AATGCCACCT GCATCGATAG CAACGGGGGC ATCTGCAACG GCCGAGGCTA CTGTGAGTGT 
GGCCGTTGTC ACTGCAACCA GCAGTCGCTC TACACGGACA CCACCTGTGA GATCAACTAC 
TCTGCGATAC GCCTGGGTCT CTGTGAGGAT CTCCGCTCCT GCGTACAGTG CCAGGCCTGG 
GGCACCGGGG AGAAGAAAGG GCGCGCGTGT GACGATTGCC CCTTTAAAGT CAAGATGGTA 
GACGAGCTTA AGAAAGCAGA AGAGGTGGTG GAGTACTGCT CCTTCCGGGA TGAGGATGAC 
GACTGCACTT ACAGCTACAA CGTGGAGGGC GATGGCAGCC CTGGGCCCAA CAGCACAGTC 
CTGGTCCACA AAAAGAAAGA CTGCCCTCCC GGCTCCTTCT GGTGGCTCAT CCCCCTGCTC 
ATCTTCCTCC TGTTGCTCCT GGCGTTGCTT CTGCTGCTCT GCTGGAAATA CTGTGCCTGC 
TGCAAAGCCT GCCTGGGGCT TCTTCCTTGC TGCAACCGAG GTCACATGGT GGGCTTTAAG 
GAAGATCACT ATATGCTTCG GGAGAACCTG ATGGCCTCTG ACCACCTGGA CACGCCCATG 
CTACGAAGCG GGAACCTCAA GGGACGAGAC ACAGTCCGCT GGAAGATCAC CAACAATGTG 
CAGCGCCCTG GCTTTGCCAC CCATGCCGCC AGCACCAGCC CCACGGAGCT CGTACCCTAC 
GGGCTGTCCC TGCGCCTTGG CCGCCTCTGC ACTGAGAACC TTATGAAGCC GGGCACCCGA 
GAGTGTGACC AGCTACGCCA GGAGGTGGAG GAAAATCTGA ATGAGGTGTA TAGACAGGTC 
AGCGGCGCAC ACAAGCTCCA GCAGACGAAG TTCCGACAGC AGCCCAACAC CGGGAAAAAG 
CAAGACCACA CCATTGTGGA CACAGTGTTG CTGGCGCCCC GCTCCGCCAA GCAGATGCTG 
CTGAAGCTGA CAGAGAAGCA GGTGGAGCAG GGGTCCTTCC ATGAACTGAA GGTGGCCCCT 
GGCTACTACA CTGTCACGGC AGAGCAGGAT GCCCGGGGCA TGGTGGAGTT CCAGGAGGGC 
GTGGAGCTGG TGGATGTGCG AGTGCCCCTC TTCATCCGGC CTGAGGATGA TGATGAGAAG 
CAGCTGCTGG TGGAGGCCAT TGATGTCCCT GTGGGCACTG CCACCCTTGG TCGCCGTCTG 
GTAAACATCA CCATTATCAA GGAACAAGCT AGTGGGGTAG TGTCCTTCGA GCAGCCTGAA 
TACTCGGTGA GTCGTGGAGA CCAGGTGGCC CGCATCCCTG TCATCCGGCA CATCCTGGAC 
AATGGCAAGT CCCAGGTCTC CTATAGCACA CAGGATAATA CAGCACACGG ACACCGGGAT 
TATGTCCCTG TGGAGGGAGA GCTGCTGTTC CATCCTGGGG AGACCTGGAA GGAGTTGCAG 
GTGAAGCTAC TGGAGCTGCA GGAGGTCGAC TCCCTCCTGC GTGGCCGCCA GGTCCGCCGC 
TTCCAAGTCC AACTCAGCAA CCCCAAGTTC GGAGCCCGCC TGGGCCAGCC CAGCACAACC 
ACCGTTATTC TCGATGAAAC GGACAGGAGT CTCATAAATC AAACACTTTC ATCGCCTCCG 
CCACCCCATG GAGACCTGGG CGCCCCACAG AACCCCAATG CCAAGGCTGC CGGGTCCAGG 
AAGATCCATT TCAACTGGCT GCCCCCTCCT GGCAAGCCAA TGGGGTACAG GGTGAAGTAC 
TGGATCCAGG GCGACTCTGA GTCTGAAGCC CACCTTCTAG ACAGCAAGGT GCCCTCAGTG 
GAACTCACCA ACCTGTATCC CTATTGCGAC TACGAAATGA AGGTGTGTGC CTATGGGGCA 
CAGGGTGAGG GGCCCTATAG CTCACTGGTG TCCTGCCGCA CCCACCAGGA AGTACCCAGC 
GAGCCAGGGA GGCTGGCTTT CAATGTAGTC TCTTCTACGG TGACGCAGCT GAGCTGGGCA 
GAGCCAGCTG AGACCAATGG CGAGATCACA GCCTACGAGG TCTGCTATGG ACTGGTCAAT 
GAGGACAACC GACCCATTGG ACCTATGAAG AAGGTGCTCG TGGACAACCC CAAGAACCGG 
ATGCTGCTCA TTGAGAATCT GCGAGAATCC CAGCCATACC GATACACGGT TAAGGCGCGC 
AATGGGGCAG GATGGGGACC CGAGAGAGAG GCTATCATCA ACCTGGCTAC ACAGCCCAAG 
CGGCCCATGT CCATCCCTAT CATCCCAGAC ATCCCCATAG TGGACGCCCA GGGTGGAGAA 
GACTACGAAA ACTTCCTTAT GTACAGTGAT GACGTCCTGC GGTCCCCAGC CAGCAGCCAG 
AGGCCCAGCG TTTCTGATGA CACTGGCTGC GGCTGGAAGT TCGAGCCTCT GCTCGGGGAG 
GAGCTGGACC TGCGGCGCGT CACGTGGCGG CTACCCCCGG AACTCATCCC GCGCCTGTCC 
GCCAGCAGCG GGCGCTCTGA CGAAGACGGC AGCGTTGCGG GGGGCGTGGA AGGGGAGGGC 
AGCGGCTGGA TCCGCGGGGC TACGCCCCGA CCCCCGGGAG AGCACCTGGT GAATGGCCGG 
ATGGACTTTG CCTATCCAGG CAGCGCCAAC TCCCTGCACA GAATGACTGC AGCCAATGTG 
GCCTATGGCA CGCATCTGAG CCCACACCTG TCCCACCGAG TGCTGAGCAC GTCCTCCACC 
CTTACCCGGG ACTACCACTC TCTGACACGC ACAGAGCACT CCCACTCAGG CACACTTCCC 
AGGGACTACT CCACCCTCAC TTCCCTTTCC TCCCAAGGCC TCCCTCCTAT CTGGGAAGAT 
GGGAGGAGCA GGCTTCCGCT GTCCTGGACT CTTGGGTCCT TGAGCCGGGC TCACATGAAG 
GGTGTGCCCG CATCCAGGGG TTCACCAGAC TCTATAATCC TGGCCGGGCA GTCAGCAGCA 
CCCTCCTGGG GTACAGATTC CCGTGGGGCT GTGGGTGTGC CTGACACACC CACTCGGCTG 
GTGTTCTCTG CCCTGGGGCC CACGTCTTTG AAGGTGAGCT GGCAGGAGCC ACAGTGTGAT 
CGGATGCTGC TGGGCTACAG TGTGGAATAC CAGCTACTAA ACGGCGGCGA GATGCATCGG 
CTCAACATCC CTAACCCTGG CCAAACCTCG GTGGTGGTAG AGGATCTCCT GCCTAACCAC 
TCCTATGTGT TCCGGGTACG GGCCCAGAGC CAGGAGGGCT GGGGCCGAGA GCGAGAGGGT 
GTCATCACCA TCGAGTCCCA GGTGCACCCG CAGAGCCCTC TCTGCCCCCT GCCAGGCTCA 
GCCTTCACTC TGAGCACCCC CAGCGCCCCG GGACCGCTGG TGTTCACTGC CCTAAGCCCA 
GACTCCCTGC AGCTCAGCTG GGAGCGGCCG AGGAGGCCCA ATGGAGATAT CCTTGGCTAC 
CTGGTGACCT GTGAGATGGC CCAAGGAGGA GCACCAGCCA GGACCTTCCG GGTGGACGGA 
GACAACCCTG AGAGCCGGTT GACTGTACCT GGCCTCAGTG AGAACGTTCC TTACAAGTTC 
AAGGTTCAGG CCAGGACGAC CGAGGGCTTC GGGCCAGAGC GTGAGGGTAT CATCACCATC 
GAGTCTCAGG TTGGAGGCCC CTTCCCACAG CTGGGCAGCC ATTCTGGGCT CTTCCAGAAC 
CCAGTGCAAA GCGAGTTCAG CAGCGTGACC AGCACGCACA GCACCACGAC TGAGCCCTTC 
CTCATGGATG GTCTAACCCT GGGGACCCAG CGCCTGGAAG CAGGAGGCTC CCTCACCCGG 
CATGTGACCC AGGAATTCGT GACCCGGACC TTAACGGCCA GTGGCTCTCT CAGCACTCAT 
ATGGACCAAC AGTTCTTCCA AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itgb4 ( XM_017314351.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169840.1 Mus musculus integrin beta 4 (Itgb4), transcript variant X3, mRNA.

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CloneID	OMu77783
Clone ID Related Accession (Same CDS sequence)	XM_017314351.1 , XM_017314351.2
Accession Version	XM_017314351.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	integrin beta-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314351.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGC CCTGTTGCAG CCCATGGGTG AAGCTGCTGC TGCTGGCAGC AATGCTGAGT 
GCCAGCCTCC CTGGAGACCT GGCCAACCGC TGCAAGAAGG CTCAGGTGAA GAGCTGTACC 
GAGTGCATCC GGGTGGACAA GAGCTGTGCC TACTGCACAG ACGAGCTGTT CAAGGAGAGG 
CGCTGCAACA CCCAGGCGGA GCTTCTGGCT GCAGGCTGCA GGGGAGAGAG CATCCTGGTC 
ATGGAGAGCA GCCTTGAAAT CACAGAGAAC ACCCAGATCG ACACCAGCCT GCACCGCAGC 
CAGGTATCTC CCCAAGGCCT GCAAGTCCGG CTGCGGCCGG GTGAGGAGCG CAGCTTTGTG 
TTCCAGGTGT TTGAGCCCCT GGAGAGCCCC GTGGATCTGT ATATCCTCAT GGACTTCTCC 
AACTCCATGT CTGACGATCT GGACAACCTC AAGCAGATGG GGCAGAACCT GGCCAAGATC 
CTGCGCCAGC TCACCAGCGA CTACACCATT GGATTTGGAA AGTTTGTGGA CAAAGTCAGC 
GTCCCACAGA CAGACATGAG GCCCGAGAAA CTGAAGGAGC CCTGGCCCAA CAGTGATCCC 
CCGTTCTCCT TCAAGAACGT TATCAGCTTA ACGGAGAATG TGGAAGAATT CTGGAACAAA 
CTGCAAGGAG AACGCATCTC AGGCAACCTG GACGCTCCTG AAGGGGGCTT TGATGCCATC 
CTGCAGACAG CTGTGTGCAC AAGGGACATT GGCTGGAGGG CTGACAGCAC CCACCTGCTG 
GTGTTCTCCA CCGAGTCTGC CTTCCACTAC GAGGCTGATG GTGCCAACGT GCTGGCCGGC 
ATCATGAACC GCAATGATGA GAAATGCCAC CTGGACGCCT CGGGCGCCTA CACCCAATAC 
AAGACACAGG ACTACCCATC AGTGCCCACG CTGGTTCGCC TGCTTGCCAA GCATAACATC 
ATCCCCATCT TTGCTGTCAC CAACTACTCT TACAGCTACT ATGAGAAGCT CCATAAGTAT 
TTCCCCGTCT CCTCTCTGGG CGTCCTGCAG GAGGATTCAT CCAACATCGT GGAGCTGCTG 
GAGGAGGCCT TCTATCGAAT TCGCTCCAAC CTGGACATCC GGGCTCTGGA CAGCCCCAGA 
GGCCTGAGAA CAGAGGTCAC CTCCGATACT CTCCAGAAGA CGGAGACTGG GTCCTTTCAC 
ATCAAGCGGG GGGAAGTGGG CACATACAAT GTGCATCTCC GGGCAGTGGA GGACATAGAT 
GGGACACATG TGTGCCAGCT GGCTAAAGAA GACCAAGGGG GCAACATCCA CCTGAAACCC 
TCCTTCTCTG ATGGCCTCCG GATGGACGCG AGTGTGATCT GTGACGTGTG CCCCTGTGAG 
CTGCAAAAGG AAGTTCGATC AGCTCGCTGT CACTTCAGAG GAGACTTCAT GTGTGGACAC 
TGTGTGTGCA ATGAGGGCTG GAGTGGCAAA ACCTGCAACT GCTCCACCGG CTCTCTGAGT 
GACACACAGC CCTGCCTGCG TGAGGGTGAG GACAAACCGT GCTCGGGCCA CGGCGAGTGC 
CAGTGCGGAC GCTGTGTGTG CTATGGTGAA GGCCGCTACG AGGGTCACTT CTGCGAGTAT 
GACAACTTCC AGTGTCCCCG GACCTCTGGA TTCCTGTGCA ATGACCGGGG TCGCTGTTCT 
ATGGGAGAGT GTGTGTGTGA GCCTGGTTGG ACAGGCCGCA GCTGTGACTG TCCCCTCAGC 
AATGCCACCT GCATCGATAG CAACGGGGGC ATCTGCAACG GCCGAGGCTA CTGTGAGTGT 
GGCCGTTGTC ACTGCAACCA GCAGTCGCTC TACACGGACA CCACCTGTGA GATCAACTAC 
TCTGCGATAC GCCTGGGTCT CTGTGAGGAT CTCCGCTCCT GCGTACAGTG CCAGGCCTGG 
GGCACCGGGG AGAAGAAAGG GCGCGCGTGT GACGATTGCC CCTTTAAAGT CAAGATGGTA 
GACGAGCTTA AGAAAGCAGA AGAGGTGGTG GAGTACTGCT CCTTCCGGGA TGAGGATGAC 
GACTGCACTT ACAGCTACAA CGTGGAGGGC GATGGCAGCC CTGGGCCCAA CAGCACAGTC 
CTGGTCCACA AAAAGAAAGA CTGCCCTCCC GGCTCCTTCT GGTGGCTCAT CCCCCTGCTC 
ATCTTCCTCC TGTTGCTCCT GGCGTTGCTT CTGCTGCTCT GCTGGAAATA CTGTGCCTGC 
TGCAAAGCCT GCCTGGGGCT TCTTCCTTGC TGCAACCGAG GTCACATGGT GGGCTTTAAG 
GAAGATCACT ATATGCTTCG GGAGAACCTG ATGGCCTCTG ACCACCTGGA CACGCCCATG 
CTACGAAGCG GGAACCTCAA GGGACGAGAC ACAGTCCGCT GGAAGATCAC CAACAATGTG 
CAGCGCCCTG GCTTTGCCAC CCATGCCGCC AGCACCAGCC CCACGGAGCT CGTACCCTAC 
GGGCTGTCCC TGCGCCTTGG CCGCCTCTGC ACTGAGAACC TTATGAAGCC GGGCACCCGA 
GAGTGTGACC AGCTACGCCA GGAGGTGGAG GAAAATCTGA ATGAGGTGTA TAGACAGGTC 
AGCGGCGCAC ACAAGCTCCA GCAGACGAAG TTCCGACAGC AGCCCAACAC CGGGAAAAAG 
CAAGACCACA CCATTGTGGA CACAGTGTTG CTGGCGCCCC GCTCCGCCAA GCAGATGCTG 
CTGAAGCTGA CAGAGAAGCA GGTGGAGCAG GGGTCCTTCC ATGAACTGAA GGTGGCCCCT 
GGCTACTACA CTGTCACGGC AGAGCAGGAT GCCCGGGGCA TGGTGGAGTT CCAGGAGGGC 
GTGGAGCTGG TGGATGTGCG AGTGCCCCTC TTCATCCGGC CTGAGGATGA TGATGAGAAG 
CAGCTGCTGG TGGAGGCCAT TGATGTCCCT GTGGGCACTG CCACCCTTGG TCGCCGTCTG 
GTAAACATCA CCATTATCAA GGAACAAGCT AGTGGGGTAG TGTCCTTCGA GCAGCCTGAA 
TACTCGGTGA GTCGTGGAGA CCAGGTGGCC CGCATCCCTG TCATCCGGCA CATCCTGGAC 
AATGGCAAGT CCCAGGTCTC CTATAGCACA CAGGATAATA CAGCACACGG ACACCGGGAT 
TATGTCCCTG TGGAGGGAGA GCTGCTGTTC CATCCTGGGG AGACCTGGAA GGAGTTGCAG 
GTGAAGCTAC TGGAGCTGCA GGAGGTCGAC TCCCTCCTGC GTGGCCGCCA GGTCCGCCGC 
TTCCAAGTCC AACTCAGCAA CCCCAAGTTC GGAGCCCGCC TGGGCCAGCC CAGCACAACC 
ACCGTTATTC TCGATGAAAC GGACAGGAGT CTCATAAATC AAACACTTTC ATCGCCTCCG 
CCACCCCATG GAGACCTGGG CGCCCCACAG AACCCCAATG CCAAGGCTGC CGGGTCCAGG 
AAGATCCATT TCAACTGGCT GCCCCCTCCT GGCAAGCCAA TGGGGTACAG GGTGAAGTAC 
TGGATCCAGG GCGACTCTGA GTCTGAAGCC CACCTTCTAG ACAGCAAGGT GCCCTCAGTG 
GAACTCACCA ACCTGTATCC CTATTGCGAC TACGAAATGA AGGTGTGTGC CTATGGGGCA 
CAGGGTGAGG GGCCCTATAG CTCACTGGTG TCCTGCCGCA CCCACCAGGA AGTACCCAGC 
GAGCCAGGGA GGCTGGCTTT CAATGTAGTC TCTTCTACGG TGACGCAGCT GAGCTGGGCA 
GAGCCAGCTG AGACCAATGG CGAGATCACA GCCTACGAGG TCTGCTATGG ACTGGTCAAT 
GAGGACAACC GACCCATTGG ACCTATGAAG AAGGTGCTCG TGGACAACCC CAAGAACCGG 
ATGCTGCTCA TTGAGAATCT GCGAGAATCC CAGCCATACC GATACACGGT TAAGGCGCGC 
AATGGGGCAG GATGGGGACC CGAGAGAGAG GCTATCATCA ACCTGGCTAC ACAGCCCAAG 
CGGCCCATGT CCATCCCTAT CATCCCAGAC ATCCCCATAG TGGACGCCCA GGGTGGAGAA 
GACTACGAAA ACTTCCTTAT GTACAGTGAT GACGTCCTGC GGTCCCCAGC CAGCAGCCAG 
AGGCCCAGCG TTTCTGATGA CACTGGCTGC GGCTGGAAGT TCGAGCCTCT GCTCGGGGAG 
GAGCTGGACC TGCGGCGCGT CACGTGGCGG CTACCCCCGG AACTCATCCC GCGCCTGTCC 
GCCAGCAGCG GGCGCTCTGA CGAAGACGGC AGCGTTGCGG GGGGCGTGGA AGGGGAGGGC 
AGCGGCTGGA TCCGCGGGGC TACGCCCCGA CCCCCGGGAG AGCACCTGGT GAATGGCCGG 
ATGGACTTTG CCTATCCAGG CAGCGCCAAC TCCCTGCACA GAATGACTGC AGCCAATGTG 
GCCTATGGCA CGCATCTGAG CCCACACCTG TCCCACCGAG TGCTGAGCAC GTCCTCCACC 
CTTACCCGGG ACTACCACTC TCTGACACGC ACAGAGCACT CCCACTCAGG CACACTTCCC 
AGGGACTACT CCACCCTCAC TTCCCTTTCC TCCCAAGGCC TCCCTCCTAT CTGGGAAGAT 
GGGAGGAGCA GGCTTCCGCT GTCCTGGACT CTTGGGTCCT TGAGCCGGGC TCACATGAAG 
GGTGTGCCCG CATCCAGGGG TTCACCAGAC TCTATAATCC TGGCCGGGCA GTCAGCAGCA 
CCCTCCTGGG GTACAGATTC CCGTGGGGCT GTGGGTGTGC CTGACACACC CACTCGGCTG 
GTGTTCTCTG CCCTGGGGCC CACGTCTTTG AAGGTGAGCT GGCAGGAGCC ACAGTGTGAT 
CGGATGCTGC TGGGCTACAG TGTGGAATAC CAGCTACTAA ACGGCGGCGA GATGCATCGG 
CTCAACATCC CTAACCCTGG CCAAACCTCG GTGGTGGTAG AGGATCTCCT GCCTAACCAC 
TCCTATGTGT TCCGGGTACG GGCCCAGAGC CAGGAGGGCT GGGGCCGAGA GCGAGAGGGT 
GTCATCACCA TCGAGTCCCA GGTGCACCCG CAGAGCCCTC TCTGCCCCCT GCCAGGCTCA 
GCCTTCACTC TGAGCACCCC CAGCGCCCCG GGACCGCTGG TGTTCACTGC CCTAAGCCCA 
GACTCCCTGC AGCTCAGCTG GGAGCGGCCG AGGAGGCCCA ATGGAGATAT CCTTGGCTAC 
CTGGTGACCT GTGAGATGGC CCAAGGAGGA GCACCAGCCA GGACCTTCCG GGTGGACGGA 
GACAACCCTG AGAGCCGGTT GACTGTACCT GGCCTCAGTG AGAACGTTCC TTACAAGTTC 
AAGGTTCAGG CCAGGACGAC CGAGGGCTTC GGGCCAGAGC GTGAGGGTAT CATCACCATC 
GAGTCTCAGG TTGGAGGCCC CTTCCCACAG CTGGGCAGCC ATTCTGGGCT CTTCCAGAAC 
CCAGTGCAAA GCGAGTTCAG CAGCGTGACC AGCACGCACA GCACCACGAC TGAGCCCTTC 
CTCATGGATG GTCTAACCCT GGGGACCCAG CGCCTGGAAG CAGGAGGCTC CCTCACCCGG 
CATGTGACCC AGGAATTCGT GACCCGGACC TTAACGGCCA GTGGCTCTCT CAGCACTCAT 
ATGGACCAAC AGTTCTTCCA AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169840.1
CDS	436..6042
Translation	MAGPCCSPWVKLLLLAAMLSASLPGDLANRCKKAQVKSCTECIRVDKSCAYCTDELFKERRCNTQAELLAAGCRGESILVMESSLEITENTQIDTSLHRSQVSPQGLQVRLRPGEERSFVFQVFEPLESPVDLYILMDFSNSMSDDLDNLKQMGQNLAKILRQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTENVEEFWNKLQGERISGNLDAPEGGFDAILQTAVCTRDIGWRADSTHLLVFSTESAFHYEADGANVLAGIMNRNDEKCHLDASGAYTQYKTQDYPSVPTLVRLLAKHNIIPIFAVTNYSYSYYEKLHKYFPVSSLGVLQEDSSNIVELLEEAFYRIRSNLDIRALDSPRGLRTEVTSDTLQKTETGSFHIKRGEVGTYNVHLRAVEDIDGTHVCQLAKEDQGGNIHLKPSFSDGLRMDASVICDVCPCELQKEVRSARCHFRGDFMCGHCVCNEGWSGKTCNCSTGSLSDTQPCLREGEDKPCSGHGECQCGRCVCYGEGRYEGHFCEYDNFQCPRTSGFLCNDRGRCSMGECVCEPGWTGRSCDCPLSNATCIDSNGGICNGRGYCECGRCHCNQQSLYTDTTCEINYSAIRLGLCEDLRSCVQCQAWGTGEKKGRACDDCPFKVKMVDELKKAEEVVEYCSFRDEDDDCTYSYNVEGDGSPGPNSTVLVHKKKDCPPGSFWWLIPLLIFLLLLLALLLLLCWKYCACCKACLGLLPCCNRGHMVGFKEDHYMLRENLMASDHLDTPMLRSGNLKGRDTVRWKITNNVQRPGFATHAASTSPTELVPYGLSLRLGRLCTENLMKPGTRECDQLRQEVEENLNEVYRQVSGAHKLQQTKFRQQPNTGKKQDHTIVDTVLLAPRSAKQMLLKLTEKQVEQGSFHELKVAPGYYTVTAEQDARGMVEFQEGVELVDVRVPLFIRPEDDDEKQLLVEAIDVPVGTATLGRRLVNITIIKEQASGVVSFEQPEYSVSRGDQVARIPVIRHILDNGKSQVSYSTQDNTAHGHRDYVPVEGELLFHPGETWKELQVKLLELQEVDSLLRGRQVRRFQVQLSNPKFGARLGQPSTTTVILDETDRSLINQTLSSPPPPHGDLGAPQNPNAKAAGSRKIHFNWLPPPGKPMGYRVKYWIQGDSESEAHLLDSKVPSVELTNLYPYCDYEMKVCAYGAQGEGPYSSLVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYGLVNEDNRPIGPMKKVLVDNPKNRMLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDIPIVDAQGGEDYENFLMYSDDVLRSPASSQRPSVSDDTGCGWKFEPLLGEELDLRRVTWRLPPELIPRLSASSGRSDEDGSVAGGVEGEGSGWIRGATPRPPGEHLVNGRMDFAYPGSANSLHRMTAANVAYGTHLSPHLSHRVLSTSSTLTRDYHSLTRTEHSHSGTLPRDYSTLTSLSSQGLPPIWEDGRSRLPLSWTLGSLSRAHMKGVPASRGSPDSIILAGQSAAPSWGTDSRGAVGVPDTPTRLVFSALGPTSLKVSWQEPQCDRMLLGYSVEYQLLNGGEMHRLNIPNPGQTSVVVEDLLPNHSYVFRVRAQSQEGWGREREGVITIESQVHPQSPLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPNGDILGYLVTCEMAQGGAPARTFRVDGDNPESRLTVPGLSENVPYKFKVQARTTEGFGPEREGIITIESQVGGPFPQLGSHSGLFQNPVQSEFSSVTSTHSTTTEPFLMDGLTLGTQRLEAGGSLTRHVTQEFVTRTLTASGSLSTHMDQQFFQT

Target ORF information:

RefSeq Version	XM_017314351.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus integrin beta 4 (Itgb4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314351.2

ORF Insert Sequence:

ATGGCAGGGC CCTGTTGCAG CCCATGGGTG AAGCTGCTGC TGCTGGCAGC AATGCTGAGT 
GCCAGCCTCC CTGGAGACCT GGCCAACCGC TGCAAGAAGG CTCAGGTGAA GAGCTGTACC 
GAGTGCATCC GGGTGGACAA GAGCTGTGCC TACTGCACAG ACGAGCTGTT CAAGGAGAGG 
CGCTGCAACA CCCAGGCGGA GCTTCTGGCT GCAGGCTGCA GGGGAGAGAG CATCCTGGTC 
ATGGAGAGCA GCCTTGAAAT CACAGAGAAC ACCCAGATCG ACACCAGCCT GCACCGCAGC 
CAGGTATCTC CCCAAGGCCT GCAAGTCCGG CTGCGGCCGG GTGAGGAGCG CAGCTTTGTG 
TTCCAGGTGT TTGAGCCCCT GGAGAGCCCC GTGGATCTGT ATATCCTCAT GGACTTCTCC 
AACTCCATGT CTGACGATCT GGACAACCTC AAGCAGATGG GGCAGAACCT GGCCAAGATC 
CTGCGCCAGC TCACCAGCGA CTACACCATT GGATTTGGAA AGTTTGTGGA CAAAGTCAGC 
GTCCCACAGA CAGACATGAG GCCCGAGAAA CTGAAGGAGC CCTGGCCCAA CAGTGATCCC 
CCGTTCTCCT TCAAGAACGT TATCAGCTTA ACGGAGAATG TGGAAGAATT CTGGAACAAA 
CTGCAAGGAG AACGCATCTC AGGCAACCTG GACGCTCCTG AAGGGGGCTT TGATGCCATC 
CTGCAGACAG CTGTGTGCAC AAGGGACATT GGCTGGAGGG CTGACAGCAC CCACCTGCTG 
GTGTTCTCCA CCGAGTCTGC CTTCCACTAC GAGGCTGATG GTGCCAACGT GCTGGCCGGC 
ATCATGAACC GCAATGATGA GAAATGCCAC CTGGACGCCT CGGGCGCCTA CACCCAATAC 
AAGACACAGG ACTACCCATC AGTGCCCACG CTGGTTCGCC TGCTTGCCAA GCATAACATC 
ATCCCCATCT TTGCTGTCAC CAACTACTCT TACAGCTACT ATGAGAAGCT CCATAAGTAT 
TTCCCCGTCT CCTCTCTGGG CGTCCTGCAG GAGGATTCAT CCAACATCGT GGAGCTGCTG 
GAGGAGGCCT TCTATCGAAT TCGCTCCAAC CTGGACATCC GGGCTCTGGA CAGCCCCAGA 
GGCCTGAGAA CAGAGGTCAC CTCCGATACT CTCCAGAAGA CGGAGACTGG GTCCTTTCAC 
ATCAAGCGGG GGGAAGTGGG CACATACAAT GTGCATCTCC GGGCAGTGGA GGACATAGAT 
GGGACACATG TGTGCCAGCT GGCTAAAGAA GACCAAGGGG GCAACATCCA CCTGAAACCC 
TCCTTCTCTG ATGGCCTCCG GATGGACGCG AGTGTGATCT GTGACGTGTG CCCCTGTGAG 
CTGCAAAAGG AAGTTCGATC AGCTCGCTGT CACTTCAGAG GAGACTTCAT GTGTGGACAC 
TGTGTGTGCA ATGAGGGCTG GAGTGGCAAA ACCTGCAACT GCTCCACCGG CTCTCTGAGT 
GACACACAGC CCTGCCTGCG TGAGGGTGAG GACAAACCGT GCTCGGGCCA CGGCGAGTGC 
CAGTGCGGAC GCTGTGTGTG CTATGGTGAA GGCCGCTACG AGGGTCACTT CTGCGAGTAT 
GACAACTTCC AGTGTCCCCG GACCTCTGGA TTCCTGTGCA ATGACCGGGG TCGCTGTTCT 
ATGGGAGAGT GTGTGTGTGA GCCTGGTTGG ACAGGCCGCA GCTGTGACTG TCCCCTCAGC 
AATGCCACCT GCATCGATAG CAACGGGGGC ATCTGCAACG GCCGAGGCTA CTGTGAGTGT 
GGCCGTTGTC ACTGCAACCA GCAGTCGCTC TACACGGACA CCACCTGTGA GATCAACTAC 
TCTGCGATAC GCCTGGGTCT CTGTGAGGAT CTCCGCTCCT GCGTACAGTG CCAGGCCTGG 
GGCACCGGGG AGAAGAAAGG GCGCGCGTGT GACGATTGCC CCTTTAAAGT CAAGATGGTA 
GACGAGCTTA AGAAAGCAGA AGAGGTGGTG GAGTACTGCT CCTTCCGGGA TGAGGATGAC 
GACTGCACTT ACAGCTACAA CGTGGAGGGC GATGGCAGCC CTGGGCCCAA CAGCACAGTC 
CTGGTCCACA AAAAGAAAGA CTGCCCTCCC GGCTCCTTCT GGTGGCTCAT CCCCCTGCTC 
ATCTTCCTCC TGTTGCTCCT GGCGTTGCTT CTGCTGCTCT GCTGGAAATA CTGTGCCTGC 
TGCAAAGCCT GCCTGGGGCT TCTTCCTTGC TGCAACCGAG GTCACATGGT GGGCTTTAAG 
GAAGATCACT ATATGCTTCG GGAGAACCTG ATGGCCTCTG ACCACCTGGA CACGCCCATG 
CTACGAAGCG GGAACCTCAA GGGACGAGAC ACAGTCCGCT GGAAGATCAC CAACAATGTG 
CAGCGCCCTG GCTTTGCCAC CCATGCCGCC AGCACCAGCC CCACGGAGCT CGTACCCTAC 
GGGCTGTCCC TGCGCCTTGG CCGCCTCTGC ACTGAGAACC TTATGAAGCC GGGCACCCGA 
GAGTGTGACC AGCTACGCCA GGAGGTGGAG GAAAATCTGA ATGAGGTGTA TAGACAGGTC 
AGCGGCGCAC ACAAGCTCCA GCAGACGAAG TTCCGACAGC AGCCCAACAC CGGGAAAAAG 
CAAGACCACA CCATTGTGGA CACAGTGTTG CTGGCGCCCC GCTCCGCCAA GCAGATGCTG 
CTGAAGCTGA CAGAGAAGCA GGTGGAGCAG GGGTCCTTCC ATGAACTGAA GGTGGCCCCT 
GGCTACTACA CTGTCACGGC AGAGCAGGAT GCCCGGGGCA TGGTGGAGTT CCAGGAGGGC 
GTGGAGCTGG TGGATGTGCG AGTGCCCCTC TTCATCCGGC CTGAGGATGA TGATGAGAAG 
CAGCTGCTGG TGGAGGCCAT TGATGTCCCT GTGGGCACTG CCACCCTTGG TCGCCGTCTG 
GTAAACATCA CCATTATCAA GGAACAAGCT AGTGGGGTAG TGTCCTTCGA GCAGCCTGAA 
TACTCGGTGA GTCGTGGAGA CCAGGTGGCC CGCATCCCTG TCATCCGGCA CATCCTGGAC 
AATGGCAAGT CCCAGGTCTC CTATAGCACA CAGGATAATA CAGCACACGG ACACCGGGAT 
TATGTCCCTG TGGAGGGAGA GCTGCTGTTC CATCCTGGGG AGACCTGGAA GGAGTTGCAG 
GTGAAGCTAC TGGAGCTGCA GGAGGTCGAC TCCCTCCTGC GTGGCCGCCA GGTCCGCCGC 
TTCCAAGTCC AACTCAGCAA CCCCAAGTTC GGAGCCCGCC TGGGCCAGCC CAGCACAACC 
ACCGTTATTC TCGATGAAAC GGACAGGAGT CTCATAAATC AAACACTTTC ATCGCCTCCG 
CCACCCCATG GAGACCTGGG CGCCCCACAG AACCCCAATG CCAAGGCTGC CGGGTCCAGG 
AAGATCCATT TCAACTGGCT GCCCCCTCCT GGCAAGCCAA TGGGGTACAG GGTGAAGTAC 
TGGATCCAGG GCGACTCTGA GTCTGAAGCC CACCTTCTAG ACAGCAAGGT GCCCTCAGTG 
GAACTCACCA ACCTGTATCC CTATTGCGAC TACGAAATGA AGGTGTGTGC CTATGGGGCA 
CAGGGTGAGG GGCCCTATAG CTCACTGGTG TCCTGCCGCA CCCACCAGGA AGTACCCAGC 
GAGCCAGGGA GGCTGGCTTT CAATGTAGTC TCTTCTACGG TGACGCAGCT GAGCTGGGCA 
GAGCCAGCTG AGACCAATGG CGAGATCACA GCCTACGAGG TCTGCTATGG ACTGGTCAAT 
GAGGACAACC GACCCATTGG ACCTATGAAG AAGGTGCTCG TGGACAACCC CAAGAACCGG 
ATGCTGCTCA TTGAGAATCT GCGAGAATCC CAGCCATACC GATACACGGT TAAGGCGCGC 
AATGGGGCAG GATGGGGACC CGAGAGAGAG GCTATCATCA ACCTGGCTAC ACAGCCCAAG 
CGGCCCATGT CCATCCCTAT CATCCCAGAC ATCCCCATAG TGGACGCCCA GGGTGGAGAA 
GACTACGAAA ACTTCCTTAT GTACAGTGAT GACGTCCTGC GGTCCCCAGC CAGCAGCCAG 
AGGCCCAGCG TTTCTGATGA CACTGGCTGC GGCTGGAAGT TCGAGCCTCT GCTCGGGGAG 
GAGCTGGACC TGCGGCGCGT CACGTGGCGG CTACCCCCGG AACTCATCCC GCGCCTGTCC 
GCCAGCAGCG GGCGCTCTGA CGAAGACGGC AGCGTTGCGG GGGGCGTGGA AGGGGAGGGC 
AGCGGCTGGA TCCGCGGGGC TACGCCCCGA CCCCCGGGAG AGCACCTGGT GAATGGCCGG 
ATGGACTTTG CCTATCCAGG CAGCGCCAAC TCCCTGCACA GAATGACTGC AGCCAATGTG 
GCCTATGGCA CGCATCTGAG CCCACACCTG TCCCACCGAG TGCTGAGCAC GTCCTCCACC 
CTTACCCGGG ACTACCACTC TCTGACACGC ACAGAGCACT CCCACTCAGG CACACTTCCC 
AGGGACTACT CCACCCTCAC TTCCCTTTCC TCCCAAGGCC TCCCTCCTAT CTGGGAAGAT 
GGGAGGAGCA GGCTTCCGCT GTCCTGGACT CTTGGGTCCT TGAGCCGGGC TCACATGAAG 
GGTGTGCCCG CATCCAGGGG TTCACCAGAC TCTATAATCC TGGCCGGGCA GTCAGCAGCA 
CCCTCCTGGG GTACAGATTC CCGTGGGGCT GTGGGTGTGC CTGACACACC CACTCGGCTG 
GTGTTCTCTG CCCTGGGGCC CACGTCTTTG AAGGTGAGCT GGCAGGAGCC ACAGTGTGAT 
CGGATGCTGC TGGGCTACAG TGTGGAATAC CAGCTACTAA ACGGCGGCGA GATGCATCGG 
CTCAACATCC CTAACCCTGG CCAAACCTCG GTGGTGGTAG AGGATCTCCT GCCTAACCAC 
TCCTATGTGT TCCGGGTACG GGCCCAGAGC CAGGAGGGCT GGGGCCGAGA GCGAGAGGGT 
GTCATCACCA TCGAGTCCCA GGTGCACCCG CAGAGCCCTC TCTGCCCCCT GCCAGGCTCA 
GCCTTCACTC TGAGCACCCC CAGCGCCCCG GGACCGCTGG TGTTCACTGC CCTAAGCCCA 
GACTCCCTGC AGCTCAGCTG GGAGCGGCCG AGGAGGCCCA ATGGAGATAT CCTTGGCTAC 
CTGGTGACCT GTGAGATGGC CCAAGGAGGA GCACCAGCCA GGACCTTCCG GGTGGACGGA 
GACAACCCTG AGAGCCGGTT GACTGTACCT GGCCTCAGTG AGAACGTTCC TTACAAGTTC 
AAGGTTCAGG CCAGGACGAC CGAGGGCTTC GGGCCAGAGC GTGAGGGTAT CATCACCATC 
GAGTCTCAGG TTGGAGGCCC CTTCCCACAG CTGGGCAGCC ATTCTGGGCT CTTCCAGAAC 
CCAGTGCAAA GCGAGTTCAG CAGCGTGACC AGCACGCACA GCACCACGAC TGAGCCCTTC 
CTCATGGATG GTCTAACCCT GGGGACCCAG CGCCTGGAAG CAGGAGGCTC CCTCACCCGG 
CATGTGACCC AGGAATTCGT GACCCGGACC TTAACGGCCA GTGGCTCTCT CAGCACTCAT 
ATGGACCAAC AGTTCTTCCA AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itgb4 ( NM_133663.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598424.2 Mus musculus integrin beta 4 (Itgb4), transcript variant 2, mRNA.

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CloneID	OMu17496
Clone ID Related Accession (Same CDS sequence)	NM_133663.2
Accession Version	NM_133663.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Mus musculus(house mouse)
Product	integrin beta-4 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK147424.1, AK048888.1 and AA407046.1. On or before Apr 25, 2007 this sequence version replaced NM_001005607.1, NM_133663.1. Transcript Variant: This variant (2) uses a different splice site and lacks an alternate in-frame exon in the 3' coding region, as compared to variant 1. The resulting protein (isoform 2) thus has two differences with a shorter C-terminus when compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147424.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGGGC CCTGTTGCAG CCCATGGGTG AAGCTGCTGC TGCTGGCAGC AATGCTGAGT 
GCCAGCCTCC CTGGAGACCT GGCCAACCGC TGCAAGAAGG CTCAGGTGAA GAGCTGTACC 
GAGTGCATCC GGGTGGACAA GAGCTGTGCC TACTGCACAG ACGAGCTGTT CAAGGAGAGG 
CGCTGCAACA CCCAGGCGGA GCTTCTGGCT GCAGGCTGCA GGGGAGAGAG CATCCTGGTC 
ATGGAGAGCA GCCTTGAAAT CACAGAGAAC ACCCAGATCG ACACCAGCCT GCACCGCAGC 
CAGGTATCTC CCCAAGGCCT GCAAGTCCGG CTGCGGCCGG GTGAGGAGCG CAGCTTTGTG 
TTCCAGGTGT TTGAGCCCCT GGAGAGCCCC GTGGATCTGT ATATCCTCAT GGACTTCTCC 
AACTCCATGT CTGACGATCT GGACAACCTC AAGCAGATGG GGCAGAACCT GGCCAAGATC 
CTGCGCCAGC TCACCAGCGA CTACACCATT GGATTTGGAA AGTTTGTGGA CAAAGTCAGC 
GTCCCACAGA CAGACATGAG GCCCGAGAAA CTGAAGGAGC CCTGGCCCAA CAGTGATCCC 
CCGTTCTCCT TCAAGAACGT TATCAGCTTA ACGGAGAATG TGGAAGAATT CTGGAACAAA 
CTGCAAGGAG AACGCATCTC AGGCAACCTG GACGCTCCTG AAGGGGGCTT TGATGCCATC 
CTGCAGACAG CTGTGTGCAC AAGGGACATT GGCTGGAGGG CTGACAGCAC CCACCTGCTG 
GTGTTCTCCA CCGAGTCTGC CTTCCACTAC GAGGCTGATG GTGCCAACGT GCTGGCCGGC 
ATCATGAACC GCAATGATGA GAAATGCCAC CTGGACGCCT CGGGCGCCTA CACCCAATAC 
AAGACACAGG ACTACCCATC AGTGCCCACG CTGGTTCGCC TGCTTGCCAA GCATAACATC 
ATCCCCATCT TTGCTGTCAC CAACTACTCT TACAGCTACT ATGAGAAGCT CCATAAGTAT 
TTCCCCGTCT CCTCTCTGGG CGTCCTGCAG GAGGATTCAT CCAACATCGT GGAGCTGCTG 
GAGGAGGCCT TCTATCGAAT TCGCTCCAAC CTGGACATCC GGGCTCTGGA CAGCCCCAGA 
GGCCTGAGAA CAGAGGTCAC CTCCGATACT CTCCAGAAGA CGGAGACTGG GTCCTTTCAC 
ATCAAGCGGG GGGAAGTGGG CACATACAAT GTGCATCTCC GGGCAGTGGA GGACATAGAT 
GGGACACATG TGTGCCAGCT GGCTAAAGAA GACCAAGGGG GCAACATCCA CCTGAAACCC 
TCCTTCTCTG ATGGCCTCCG GATGGACGCG AGTGTGATCT GTGACGTGTG CCCCTGTGAG 
CTGCAAAAGG AAGTTCGATC AGCTCGCTGT CACTTCAGAG GAGACTTCAT GTGTGGACAC 
TGTGTGTGCA ATGAGGGCTG GAGTGGCAAA ACCTGCAACT GCTCCACCGG CTCTCTGAGT 
GACACACAGC CCTGCCTGCG TGAGGGTGAG GACAAACCGT GCTCGGGCCA CGGCGAGTGC 
CAGTGCGGAC GCTGTGTGTG CTATGGTGAA GGCCGCTACG AGGGTCACTT CTGCGAGTAT 
GACAACTTCC AGTGTCCCCG GACCTCTGGA TTCCTGTGCA ATGACCGGGG TCGCTGTTCT 
ATGGGAGAGT GTGTGTGTGA GCCTGGTTGG ACAGGCCGCA GCTGTGACTG TCCCCTCAGC 
AATGCCACCT GCATCGATAG CAACGGGGGC ATCTGCAACG GCCGAGGCTA CTGTGAGTGT 
GGCCGTTGTC ACTGCAACCA GCAGTCGCTC TACACGGACA CCACCTGTGA GATCAACTAC 
TCTGCGATAC GCCTGGGTCT CTGTGAGGAT CTCCGCTCCT GCGTACAGTG CCAGGCCTGG 
GGCACCGGGG AGAAGAAAGG GCGCGCGTGT GACGATTGCC CCTTTAAAGT CAAGATGGTA 
GACGAGCTTA AGAAAGAAGA GGTGGTGGAG TACTGCTCCT TCCGGGATGA GGATGACGAC 
TGCACTTACA GCTACAACGT GGAGGGCGAT GGCAGCCCTG GGCCCAACAG CACAGTCCTG 
GTCCACAAAA AGAAAGACTG CCCTCCCGGC TCCTTCTGGT GGCTCATCCC CCTGCTCATC 
TTCCTCCTGT TGCTCCTGGC GTTGCTTCTG CTGCTCTGCT GGAAATACTG TGCCTGCTGC 
AAAGCCTGCC TGGGGCTTCT TCCTTGCTGC AACCGAGGTC ACATGGTGGG CTTTAAGGAA 
GATCACTATA TGCTTCGGGA GAACCTGATG GCCTCTGACC ACCTGGACAC GCCCATGCTA 
CGAAGCGGGA ACCTCAAGGG ACGAGACACA GTCCGCTGGA AGATCACCAA CAATGTGCAG 
CGCCCTGGCT TTGCCACCCA TGCCGCCAGC ACCAGCCCCA CGGAGCTCGT ACCCTACGGG 
CTGTCCCTGC GCCTTGGCCG CCTCTGCACT GAGAACCTTA TGAAGCCGGG CACCCGAGAG 
TGTGACCAGC TACGCCAGGA GGTGGAGGAA AATCTGAATG AGGTGTATAG ACAGGTCAGC 
GGCGCACACA AGCTCCAGCA GACGAAGTTC CGACAGCAGC CCAACACCGG GAAAAAGCAA 
GACCACACCA TTGTGGACAC AGTGTTGCTG GCGCCCCGCT CCGCCAAGCA GATGCTGCTG 
AAGCTGACAG AGAAGCAGGT GGAGCAGGGG TCCTTCCATG AACTGAAGGT GGCCCCTGGC 
TACTACACTG TCACGGCAGA GCAGGATGCC CGGGGCATGG TGGAGTTCCA GGAGGGCGTG 
GAGCTGGTGG ATGTGCGAGT GCCCCTCTTC ATCCGGCCTG AGGATGATGA TGAGAAGCAG 
CTGCTGGTGG AGGCCATTGA TGTCCCTGTG GGCACTGCCA CCCTTGGTCG CCGTCTGGTA 
AACATCACCA TTATCAAGGA ACAAGCTAGT GGGGTAGTGT CCTTCGAGCA GCCTGAATAC 
TCGGTGAGTC GTGGAGACCA GGTGGCCCGC ATCCCTGTCA TCCGGCACAT CCTGGACAAT 
GGCAAGTCCC AGGTCTCCTA TAGCACACAG GATAATACAG CACACGGACA CCGGGATTAT 
GTCCCTGTGG AGGGAGAGCT GCTGTTCCAT CCTGGGGAGA CCTGGAAGGA GTTGCAGGTG 
AAGCTACTGG AGCTGCAGGA GGTCGACTCC CTCCTGCGTG GCCGCCAGGT CCGCCGCTTC 
CAAGTCCAAC TCAGCAACCC CAAGTTCGGA GCCCGCCTGG GCCAGCCCAG CACAACCACC 
GTTATTCTCG GTGAGCACGA TGAAACGGAC AGGAGTCTCA TAAATCAAAC ACTTTCATCG 
CCTCCGCCAC CCCATGGAGA CCTGGGCGCC CCACAGAACC CCAATGCCAA GGCTGCCGGG 
TCCAGGAAGA TCCATTTCAA CTGGCTGCCC CCTCCTGGCA AGCCAATGGG GTACAGGGTG 
AAGTACTGGA TCCAGGGCGA CTCTGAGTCT GAAGCCCACC TTCTAGACAG CAAGGTGCCC 
TCAGTGGAAC TCACCAACCT GTATCCCTAT TGCGACTACG AAATGAAGGT GTGTGCCTAT 
GGGGCACAGG GTGAGGGGCC CTATAGCTCA CTGGTGTCCT GCCGCACCCA CCAGGAAGTA 
CCCAGCGAGC CAGGGAGGCT GGCTTTCAAT GTAGTCTCTT CTACGGTGAC GCAGCTGAGC 
TGGGCAGAGC CAGCTGAGAC CAATGGCGAG ATCACAGCCT ACGAGGTCTG CTATGGACTG 
GTCAATGAGG ACAACCGACC CATTGGACCT ATGAAGAAGG TGCTCGTGGA CAACCCCAAG 
AACCGGATGC TGCTCATTGA GAATCTGCGA GAATCCCAGC CATACCGATA CACGGTTAAG 
GCGCGCAATG GGGCAGGATG GGGACCCGAG AGAGAGGCTA TCATCAACCT GGCTACACAG 
CCCAAGCGGC CCATGTCCAT CCCTATCATC CCAGACATCC CCATAGTGGA CGCCCAGGGT 
GGAGAAGACT ACGAAAACTT CCTTATGTAC AGTGATGACG TCCTGCGGTC CCCAGCCAGC 
AGCCAGAGGC CCAGCGTTTC TGATGACACT GAGCACCTGG TGAATGGCCG GATGGACTTT 
GCCTATCCAG GCAGCGCCAA CTCCCTGCAC AGAATGACTG CAGCCAATGT GGCCTATGGC 
ACGCATCTGA GCCCACACCT GTCCCACCGA GTGCTGAGCA CGTCCTCCAC CCTTACCCGG 
GACTACCACT CTCTGACACG CACAGAGCAC TCCCACTCAG GCACACTTCC CAGGGACTAC 
TCCACCCTCA CTTCCCTTTC CTCCCAAGAT TCCCGTGGGG CTGTGGGTGT GCCTGACACA 
CCCACTCGGC TGGTGTTCTC TGCCCTGGGG CCCACGTCTT TGAAGGTGAG CTGGCAGGAG 
CCACAGTGTG ATCGGATGCT GCTGGGCTAC AGTGTGGAAT ACCAGCTACT AAACGGCGGC 
GAGATGCATC GGCTCAACAT CCCTAACCCT GGCCAAACCT CGGTGGTGGT AGAGGATCTC 
CTGCCTAACC ACTCCTATGT GTTCCGGGTA CGGGCCCAGA GCCAGGAGGG CTGGGGCCGA 
GAGCGAGAGG GTGTCATCAC CATCGAGTCC CAGGTGCACC CGCAGAGCCC TCTCTGCCCC 
CTGCCAGGCT CAGCCTTCAC TCTGAGCACC CCCAGCGCCC CGGGACCGCT GGTGTTCACT 
GCCCTAAGCC CAGACTCCCT GCAGCTCAGC TGGGAGCGGC CGAGGAGGCC CAATGGAGAT 
ATCCTTGGCT ACCTGGTGAC CTGTGAGATG GCCCAAGGAG GAGCACCAGC CAGGACCTTC 
CGGGTGGACG GAGACAACCC TGAGAGCCGG TTGACTGTAC CTGGCCTCAG TGAGAACGTT 
CCTTACAAGT TCAAGGTTCA GGCCAGGACG ACCGAGGGCT TCGGGCCAGA GCGTGAGGGT 
ATCATCACCA TCGAGTCTCA GGTTGGAGGC CCCTTCCCAC AGCTGGGCAG CCATTCTGGG 
CTCTTCCAGA ACCCAGTGCA AAGCGAGTTC AGCAGCGTGA CCAGCACGCA CAGCACCACG 
ACTGAGCCCT TCCTCATGGA TGGTCTAACC CTGGGGACCC AGCGCCTGGA AGCAGGAGGC 
TCCCTCACCC GGCATGTGAC CCAGGAATTC GTGACCCGGA CCTTAACGGC CAGTGGCTCT 
CTCAGCACTC ATATGGACCA ACAGTTCTTC CAAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598424.2
CDS	441..5699
Translation	MAGPCCSPWVKLLLLAAMLSASLPGDLANRCKKAQVKSCTECIRVDKSCAYCTDELFKERRCNTQAELLAAGCRGESILVMESSLEITENTQIDTSLHRSQVSPQGLQVRLRPGEERSFVFQVFEPLESPVDLYILMDFSNSMSDDLDNLKQMGQNLAKILRQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTENVEEFWNKLQGERISGNLDAPEGGFDAILQTAVCTRDIGWRADSTHLLVFSTESAFHYEADGANVLAGIMNRNDEKCHLDASGAYTQYKTQDYPSVPTLVRLLAKHNIIPIFAVTNYSYSYYEKLHKYFPVSSLGVLQEDSSNIVELLEEAFYRIRSNLDIRALDSPRGLRTEVTSDTLQKTETGSFHIKRGEVGTYNVHLRAVEDIDGTHVCQLAKEDQGGNIHLKPSFSDGLRMDASVICDVCPCELQKEVRSARCHFRGDFMCGHCVCNEGWSGKTCNCSTGSLSDTQPCLREGEDKPCSGHGECQCGRCVCYGEGRYEGHFCEYDNFQCPRTSGFLCNDRGRCSMGECVCEPGWTGRSCDCPLSNATCIDSNGGICNGRGYCECGRCHCNQQSLYTDTTCEINYSAIRLGLCEDLRSCVQCQAWGTGEKKGRACDDCPFKVKMVDELKKEEVVEYCSFRDEDDDCTYSYNVEGDGSPGPNSTVLVHKKKDCPPGSFWWLIPLLIFLLLLLALLLLLCWKYCACCKACLGLLPCCNRGHMVGFKEDHYMLRENLMASDHLDTPMLRSGNLKGRDTVRWKITNNVQRPGFATHAASTSPTELVPYGLSLRLGRLCTENLMKPGTRECDQLRQEVEENLNEVYRQVSGAHKLQQTKFRQQPNTGKKQDHTIVDTVLLAPRSAKQMLLKLTEKQVEQGSFHELKVAPGYYTVTAEQDARGMVEFQEGVELVDVRVPLFIRPEDDDEKQLLVEAIDVPVGTATLGRRLVNITIIKEQASGVVSFEQPEYSVSRGDQVARIPVIRHILDNGKSQVSYSTQDNTAHGHRDYVPVEGELLFHPGETWKELQVKLLELQEVDSLLRGRQVRRFQVQLSNPKFGARLGQPSTTTVILGEHDETDRSLINQTLSSPPPPHGDLGAPQNPNAKAAGSRKIHFNWLPPPGKPMGYRVKYWIQGDSESEAHLLDSKVPSVELTNLYPYCDYEMKVCAYGAQGEGPYSSLVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYGLVNEDNRPIGPMKKVLVDNPKNRMLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDIPIVDAQGGEDYENFLMYSDDVLRSPASSQRPSVSDDTEHLVNGRMDFAYPGSANSLHRMTAANVAYGTHLSPHLSHRVLSTSSTLTRDYHSLTRTEHSHSGTLPRDYSTLTSLSSQDSRGAVGVPDTPTRLVFSALGPTSLKVSWQEPQCDRMLLGYSVEYQLLNGGEMHRLNIPNPGQTSVVVEDLLPNHSYVFRVRAQSQEGWGREREGVITIESQVHPQSPLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPNGDILGYLVTCEMAQGGAPARTFRVDGDNPESRLTVPGLSENVPYKFKVQARTTEGFGPEREGIITIESQVGGPFPQLGSHSGLFQNPVQSEFSSVTSTHSTTTEPFLMDGLTLGTQRLEAGGSLTRHVTQEFVTRTLTASGSLSTHMDQQFFQT

Target ORF information:

RefSeq Version	NM_133663.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus integrin beta 4 (Itgb4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133663.2

ORF Insert Sequence:

ATGGCAGGGC CCTGTTGCAG CCCATGGGTG AAGCTGCTGC TGCTGGCAGC AATGCTGAGT 
GCCAGCCTCC CTGGAGACCT GGCCAACCGC TGCAAGAAGG CTCAGGTGAA GAGCTGTACC 
GAGTGCATCC GGGTGGACAA GAGCTGTGCC TACTGCACAG ACGAGCTGTT CAAGGAGAGG 
CGCTGCAACA CCCAGGCGGA GCTTCTGGCT GCAGGCTGCA GGGGAGAGAG CATCCTGGTC 
ATGGAGAGCA GCCTTGAAAT CACAGAGAAC ACCCAGATCG ACACCAGCCT GCACCGCAGC 
CAGGTATCTC CCCAAGGCCT GCAAGTCCGG CTGCGGCCGG GTGAGGAGCG CAGCTTTGTG 
TTCCAGGTGT TTGAGCCCCT GGAGAGCCCC GTGGATCTGT ATATCCTCAT GGACTTCTCC 
AACTCCATGT CTGACGATCT GGACAACCTC AAGCAGATGG GGCAGAACCT GGCCAAGATC 
CTGCGCCAGC TCACCAGCGA CTACACCATT GGATTTGGAA AGTTTGTGGA CAAAGTCAGC 
GTCCCACAGA CAGACATGAG GCCCGAGAAA CTGAAGGAGC CCTGGCCCAA CAGTGATCCC 
CCGTTCTCCT TCAAGAACGT TATCAGCTTA ACGGAGAATG TGGAAGAATT CTGGAACAAA 
CTGCAAGGAG AACGCATCTC AGGCAACCTG GACGCTCCTG AAGGGGGCTT TGATGCCATC 
CTGCAGACAG CTGTGTGCAC AAGGGACATT GGCTGGAGGG CTGACAGCAC CCACCTGCTG 
GTGTTCTCCA CCGAGTCTGC CTTCCACTAC GAGGCTGATG GTGCCAACGT GCTGGCCGGC 
ATCATGAACC GCAATGATGA GAAATGCCAC CTGGACGCCT CGGGCGCCTA CACCCAATAC 
AAGACACAGG ACTACCCATC AGTGCCCACG CTGGTTCGCC TGCTTGCCAA GCATAACATC 
ATCCCCATCT TTGCTGTCAC CAACTACTCT TACAGCTACT ATGAGAAGCT CCATAAGTAT 
TTCCCCGTCT CCTCTCTGGG CGTCCTGCAG GAGGATTCAT CCAACATCGT GGAGCTGCTG 
GAGGAGGCCT TCTATCGAAT TCGCTCCAAC CTGGACATCC GGGCTCTGGA CAGCCCCAGA 
GGCCTGAGAA CAGAGGTCAC CTCCGATACT CTCCAGAAGA CGGAGACTGG GTCCTTTCAC 
ATCAAGCGGG GGGAAGTGGG CACATACAAT GTGCATCTCC GGGCAGTGGA GGACATAGAT 
GGGACACATG TGTGCCAGCT GGCTAAAGAA GACCAAGGGG GCAACATCCA CCTGAAACCC 
TCCTTCTCTG ATGGCCTCCG GATGGACGCG AGTGTGATCT GTGACGTGTG CCCCTGTGAG 
CTGCAAAAGG AAGTTCGATC AGCTCGCTGT CACTTCAGAG GAGACTTCAT GTGTGGACAC 
TGTGTGTGCA ATGAGGGCTG GAGTGGCAAA ACCTGCAACT GCTCCACCGG CTCTCTGAGT 
GACACACAGC CCTGCCTGCG TGAGGGTGAG GACAAACCGT GCTCGGGCCA CGGCGAGTGC 
CAGTGCGGAC GCTGTGTGTG CTATGGTGAA GGCCGCTACG AGGGTCACTT CTGCGAGTAT 
GACAACTTCC AGTGTCCCCG GACCTCTGGA TTCCTGTGCA ATGACCGGGG TCGCTGTTCT 
ATGGGAGAGT GTGTGTGTGA GCCTGGTTGG ACAGGCCGCA GCTGTGACTG TCCCCTCAGC 
AATGCCACCT GCATCGATAG CAACGGGGGC ATCTGCAACG GCCGAGGCTA CTGTGAGTGT 
GGCCGTTGTC ACTGCAACCA GCAGTCGCTC TACACGGACA CCACCTGTGA GATCAACTAC 
TCTGCGATAC GCCTGGGTCT CTGTGAGGAT CTCCGCTCCT GCGTACAGTG CCAGGCCTGG 
GGCACCGGGG AGAAGAAAGG GCGCGCGTGT GACGATTGCC CCTTTAAAGT CAAGATGGTA 
GACGAGCTTA AGAAAGAAGA GGTGGTGGAG TACTGCTCCT TCCGGGATGA GGATGACGAC 
TGCACTTACA GCTACAACGT GGAGGGCGAT GGCAGCCCTG GGCCCAACAG CACAGTCCTG 
GTCCACAAAA AGAAAGACTG CCCTCCCGGC TCCTTCTGGT GGCTCATCCC CCTGCTCATC 
TTCCTCCTGT TGCTCCTGGC GTTGCTTCTG CTGCTCTGCT GGAAATACTG TGCCTGCTGC 
AAAGCCTGCC TGGGGCTTCT TCCTTGCTGC AACCGAGGTC ACATGGTGGG CTTTAAGGAA 
GATCACTATA TGCTTCGGGA GAACCTGATG GCCTCTGACC ACCTGGACAC GCCCATGCTA 
CGAAGCGGGA ACCTCAAGGG ACGAGACACA GTCCGCTGGA AGATCACCAA CAATGTGCAG 
CGCCCTGGCT TTGCCACCCA TGCCGCCAGC ACCAGCCCCA CGGAGCTCGT ACCCTACGGG 
CTGTCCCTGC GCCTTGGCCG CCTCTGCACT GAGAACCTTA TGAAGCCGGG CACCCGAGAG 
TGTGACCAGC TACGCCAGGA GGTGGAGGAA AATCTGAATG AGGTGTATAG ACAGGTCAGC 
GGCGCACACA AGCTCCAGCA GACGAAGTTC CGACAGCAGC CCAACACCGG GAAAAAGCAA 
GACCACACCA TTGTGGACAC AGTGTTGCTG GCGCCCCGCT CCGCCAAGCA GATGCTGCTG 
AAGCTGACAG AGAAGCAGGT GGAGCAGGGG TCCTTCCATG AACTGAAGGT GGCCCCTGGC 
TACTACACTG TCACGGCAGA GCAGGATGCC CGGGGCATGG TGGAGTTCCA GGAGGGCGTG 
GAGCTGGTGG ATGTGCGAGT GCCCCTCTTC ATCCGGCCTG AGGATGATGA TGAGAAGCAG 
CTGCTGGTGG AGGCCATTGA TGTCCCTGTG GGCACTGCCA CCCTTGGTCG CCGTCTGGTA 
AACATCACCA TTATCAAGGA ACAAGCTAGT GGGGTAGTGT CCTTCGAGCA GCCTGAATAC 
TCGGTGAGTC GTGGAGACCA GGTGGCCCGC ATCCCTGTCA TCCGGCACAT CCTGGACAAT 
GGCAAGTCCC AGGTCTCCTA TAGCACACAG GATAATACAG CACACGGACA CCGGGATTAT 
GTCCCTGTGG AGGGAGAGCT GCTGTTCCAT CCTGGGGAGA CCTGGAAGGA GTTGCAGGTG 
AAGCTACTGG AGCTGCAGGA GGTCGACTCC CTCCTGCGTG GCCGCCAGGT CCGCCGCTTC 
CAAGTCCAAC TCAGCAACCC CAAGTTCGGA GCCCGCCTGG GCCAGCCCAG CACAACCACC 
GTTATTCTCG GTGAGCACGA TGAAACGGAC AGGAGTCTCA TAAATCAAAC ACTTTCATCG 
CCTCCGCCAC CCCATGGAGA CCTGGGCGCC CCACAGAACC CCAATGCCAA GGCTGCCGGG 
TCCAGGAAGA TCCATTTCAA CTGGCTGCCC CCTCCTGGCA AGCCAATGGG GTACAGGGTG 
AAGTACTGGA TCCAGGGCGA CTCTGAGTCT GAAGCCCACC TTCTAGACAG CAAGGTGCCC 
TCAGTGGAAC TCACCAACCT GTATCCCTAT TGCGACTACG AAATGAAGGT GTGTGCCTAT 
GGGGCACAGG GTGAGGGGCC CTATAGCTCA CTGGTGTCCT GCCGCACCCA CCAGGAAGTA 
CCCAGCGAGC CAGGGAGGCT GGCTTTCAAT GTAGTCTCTT CTACGGTGAC GCAGCTGAGC 
TGGGCAGAGC CAGCTGAGAC CAATGGCGAG ATCACAGCCT ACGAGGTCTG CTATGGACTG 
GTCAATGAGG ACAACCGACC CATTGGACCT ATGAAGAAGG TGCTCGTGGA CAACCCCAAG 
AACCGGATGC TGCTCATTGA GAATCTGCGA GAATCCCAGC CATACCGATA CACGGTTAAG 
GCGCGCAATG GGGCAGGATG GGGACCCGAG AGAGAGGCTA TCATCAACCT GGCTACACAG 
CCCAAGCGGC CCATGTCCAT CCCTATCATC CCAGACATCC CCATAGTGGA CGCCCAGGGT 
GGAGAAGACT ACGAAAACTT CCTTATGTAC AGTGATGACG TCCTGCGGTC CCCAGCCAGC 
AGCCAGAGGC CCAGCGTTTC TGATGACACT GAGCACCTGG TGAATGGCCG GATGGACTTT 
GCCTATCCAG GCAGCGCCAA CTCCCTGCAC AGAATGACTG CAGCCAATGT GGCCTATGGC 
ACGCATCTGA GCCCACACCT GTCCCACCGA GTGCTGAGCA CGTCCTCCAC CCTTACCCGG 
GACTACCACT CTCTGACACG CACAGAGCAC TCCCACTCAG GCACACTTCC CAGGGACTAC 
TCCACCCTCA CTTCCCTTTC CTCCCAAGAT TCCCGTGGGG CTGTGGGTGT GCCTGACACA 
CCCACTCGGC TGGTGTTCTC TGCCCTGGGG CCCACGTCTT TGAAGGTGAG CTGGCAGGAG 
CCACAGTGTG ATCGGATGCT GCTGGGCTAC AGTGTGGAAT ACCAGCTACT AAACGGCGGC 
GAGATGCATC GGCTCAACAT CCCTAACCCT GGCCAAACCT CGGTGGTGGT AGAGGATCTC 
CTGCCTAACC ACTCCTATGT GTTCCGGGTA CGGGCCCAGA GCCAGGAGGG CTGGGGCCGA 
GAGCGAGAGG GTGTCATCAC CATCGAGTCC CAGGTGCACC CGCAGAGCCC TCTCTGCCCC 
CTGCCAGGCT CAGCCTTCAC TCTGAGCACC CCCAGCGCCC CGGGACCGCT GGTGTTCACT 
GCCCTAAGCC CAGACTCCCT GCAGCTCAGC TGGGAGCGGC CGAGGAGGCC CAATGGAGAT 
ATCCTTGGCT ACCTGGTGAC CTGTGAGATG GCCCAAGGAG GAGCACCAGC CAGGACCTTC 
CGGGTGGACG GAGACAACCC TGAGAGCCGG TTGACTGTAC CTGGCCTCAG TGAGAACGTT 
CCTTACAAGT TCAAGGTTCA GGCCAGGACG ACCGAGGGCT TCGGGCCAGA GCGTGAGGGT 
ATCATCACCA TCGAGTCTCA GGTTGGAGGC CCCTTCCCAC AGCTGGGCAG CCATTCTGGG 
CTCTTCCAGA ACCCAGTGCA AAGCGAGTTC AGCAGCGTGA CCAGCACGCA CAGCACCACG 
ACTGAGCCCT TCCTCATGGA TGGTCTAACC CTGGGGACCC AGCGCCTGGA AGCAGGAGGC 
TCCCTCACCC GGCATGTGAC CCAGGAATTC GTGACCCGGA CCTTAACGGC CAGTGGCTCT 
CTCAGCACTC ATATGGACCA ACAGTTCTTC CAAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itgb4 ( NM_001005608.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001005608.2 Mus musculus integrin beta 4 (Itgb4), transcript variant 1, mRNA.

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CloneID	OMu17669
Clone ID Related Accession (Same CDS sequence)	NM_001005608.2
Accession Version	NM_001005608.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Mus musculus(house mouse)
Product	integrin beta-4 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK147424.1, AK048888.1, BE863493.1 and AA407046.1. On Jul 26, 2006 this sequence version replaced NM_001005608.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGGGC CCTGTTGCAG CCCATGGGTG AAGCTGCTGC TGCTGGCAGC AATGCTGAGT 
GCCAGCCTCC CTGGAGACCT GGCCAACCGC TGCAAGAAGG CTCAGGTGAA GAGCTGTACC 
GAGTGCATCC GGGTGGACAA GAGCTGTGCC TACTGCACAG ACGAGCTGTT CAAGGAGAGG 
CGCTGCAACA CCCAGGCGGA GCTTCTGGCT GCAGGCTGCA GGGGAGAGAG CATCCTGGTC 
ATGGAGAGCA GCCTTGAAAT CACAGAGAAC ACCCAGATCG ACACCAGCCT GCACCGCAGC 
CAGGTATCTC CCCAAGGCCT GCAAGTCCGG CTGCGGCCGG GTGAGGAGCG CAGCTTTGTG 
TTCCAGGTGT TTGAGCCCCT GGAGAGCCCC GTGGATCTGT ATATCCTCAT GGACTTCTCC 
AACTCCATGT CTGACGATCT GGACAACCTC AAGCAGATGG GGCAGAACCT GGCCAAGATC 
CTGCGCCAGC TCACCAGCGA CTACACCATT GGATTTGGAA AGTTTGTGGA CAAAGTCAGC 
GTCCCACAGA CAGACATGAG GCCCGAGAAA CTGAAGGAGC CCTGGCCCAA CAGTGATCCC 
CCGTTCTCCT TCAAGAACGT TATCAGCTTA ACGGAGAATG TGGAAGAATT CTGGAACAAA 
CTGCAAGGAG AACGCATCTC AGGCAACCTG GACGCTCCTG AAGGGGGCTT TGATGCCATC 
CTGCAGACAG CTGTGTGCAC AAGGGACATT GGCTGGAGGG CTGACAGCAC CCACCTGCTG 
GTGTTCTCCA CCGAGTCTGC CTTCCACTAC GAGGCTGATG GTGCCAACGT GCTGGCCGGC 
ATCATGAACC GCAATGATGA GAAATGCCAC CTGGACGCCT CGGGCGCCTA CACCCAATAC 
AAGACACAGG ACTACCCATC AGTGCCCACG CTGGTTCGCC TGCTTGCCAA GCATAACATC 
ATCCCCATCT TTGCTGTCAC CAACTACTCT TACAGCTACT ATGAGAAGCT CCATAAGTAT 
TTCCCCGTCT CCTCTCTGGG CGTCCTGCAG GAGGATTCAT CCAACATCGT GGAGCTGCTG 
GAGGAGGCCT TCTATCGAAT TCGCTCCAAC CTGGACATCC GGGCTCTGGA CAGCCCCAGA 
GGCCTGAGAA CAGAGGTCAC CTCCGATACT CTCCAGAAGA CGGAGACTGG GTCCTTTCAC 
ATCAAGCGGG GGGAAGTGGG CACATACAAT GTGCATCTCC GGGCAGTGGA GGACATAGAT 
GGGACACATG TGTGCCAGCT GGCTAAAGAA GACCAAGGGG GCAACATCCA CCTGAAACCC 
TCCTTCTCTG ATGGCCTCCG GATGGACGCG AGTGTGATCT GTGACGTGTG CCCCTGTGAG 
CTGCAAAAGG AAGTTCGATC AGCTCGCTGT CACTTCAGAG GAGACTTCAT GTGTGGACAC 
TGTGTGTGCA ATGAGGGCTG GAGTGGCAAA ACCTGCAACT GCTCCACCGG CTCTCTGAGT 
GACACACAGC CCTGCCTGCG TGAGGGTGAG GACAAACCGT GCTCGGGCCA CGGCGAGTGC 
CAGTGCGGAC GCTGTGTGTG CTATGGTGAA GGCCGCTACG AGGGTCACTT CTGCGAGTAT 
GACAACTTCC AGTGTCCCCG GACCTCTGGA TTCCTGTGCA ATGACCGGGG TCGCTGTTCT 
ATGGGAGAGT GTGTGTGTGA GCCTGGTTGG ACAGGCCGCA GCTGTGACTG TCCCCTCAGC 
AATGCCACCT GCATCGATAG CAACGGGGGC ATCTGCAACG GCCGAGGCTA CTGTGAGTGT 
GGCCGTTGTC ACTGCAACCA GCAGTCGCTC TACACGGACA CCACCTGTGA GATCAACTAC 
TCTGCGATAC GCCTGGGTCT CTGTGAGGAT CTCCGCTCCT GCGTACAGTG CCAGGCCTGG 
GGCACCGGGG AGAAGAAAGG GCGCGCGTGT GACGATTGCC CCTTTAAAGT CAAGATGGTA 
GACGAGCTTA AGAAAGAAGA GGTGGTGGAG TACTGCTCCT TCCGGGATGA GGATGACGAC 
TGCACTTACA GCTACAACGT GGAGGGCGAT GGCAGCCCTG GGCCCAACAG CACAGTCCTG 
GTCCACAAAA AGAAAGACTG CCCTCCCGGC TCCTTCTGGT GGCTCATCCC CCTGCTCATC 
TTCCTCCTGT TGCTCCTGGC GTTGCTTCTG CTGCTCTGCT GGAAATACTG TGCCTGCTGC 
AAAGCCTGCC TGGGGCTTCT TCCTTGCTGC AACCGAGGTC ACATGGTGGG CTTTAAGGAA 
GATCACTATA TGCTTCGGGA GAACCTGATG GCCTCTGACC ACCTGGACAC GCCCATGCTA 
CGAAGCGGGA ACCTCAAGGG ACGAGACACA GTCCGCTGGA AGATCACCAA CAATGTGCAG 
CGCCCTGGCT TTGCCACCCA TGCCGCCAGC ACCAGCCCCA CGGAGCTCGT ACCCTACGGG 
CTGTCCCTGC GCCTTGGCCG CCTCTGCACT GAGAACCTTA TGAAGCCGGG CACCCGAGAG 
TGTGACCAGC TACGCCAGGA GGTGGAGGAA AATCTGAATG AGGTGTATAG ACAGGTCAGC 
GGCGCACACA AGCTCCAGCA GACGAAGTTC CGACAGCAGC CCAACACCGG GAAAAAGCAA 
GACCACACCA TTGTGGACAC AGTGTTGCTG GCGCCCCGCT CCGCCAAGCA GATGCTGCTG 
AAGCTGACAG AGAAGCAGGT GGAGCAGGGG TCCTTCCATG AACTGAAGGT GGCCCCTGGC 
TACTACACTG TCACGGCAGA GCAGGATGCC CGGGGCATGG TGGAGTTCCA GGAGGGCGTG 
GAGCTGGTGG ATGTGCGAGT GCCCCTCTTC ATCCGGCCTG AGGATGATGA TGAGAAGCAG 
CTGCTGGTGG AGGCCATTGA TGTCCCTGTG GGCACTGCCA CCCTTGGTCG CCGTCTGGTA 
AACATCACCA TTATCAAGGA ACAAGCTAGT GGGGTAGTGT CCTTCGAGCA GCCTGAATAC 
TCGGTGAGTC GTGGAGACCA GGTGGCCCGC ATCCCTGTCA TCCGGCACAT CCTGGACAAT 
GGCAAGTCCC AGGTCTCCTA TAGCACACAG GATAATACAG CACACGGACA CCGGGATTAT 
GTCCCTGTGG AGGGAGAGCT GCTGTTCCAT CCTGGGGAGA CCTGGAAGGA GTTGCAGGTG 
AAGCTACTGG AGCTGCAGGA GGTCGACTCC CTCCTGCGTG GCCGCCAGGT CCGCCGCTTC 
CAAGTCCAAC TCAGCAACCC CAAGTTCGGA GCCCGCCTGG GCCAGCCCAG CACAACCACC 
GTTATTCTCG ATGAAACGGA CAGGAGTCTC ATAAATCAAA CACTTTCATC GCCTCCGCCA 
CCCCATGGAG ACCTGGGCGC CCCACAGAAC CCCAATGCCA AGGCTGCCGG GTCCAGGAAG 
ATCCATTTCA ACTGGCTGCC CCCTCCTGGC AAGCCAATGG GGTACAGGGT GAAGTACTGG 
ATCCAGGGCG ACTCTGAGTC TGAAGCCCAC CTTCTAGACA GCAAGGTGCC CTCAGTGGAA 
CTCACCAACC TGTATCCCTA TTGCGACTAC GAAATGAAGG TGTGTGCCTA TGGGGCACAG 
GGTGAGGGGC CCTATAGCTC ACTGGTGTCC TGCCGCACCC ACCAGGAAGT ACCCAGCGAG 
CCAGGGAGGC TGGCTTTCAA TGTAGTCTCT TCTACGGTGA CGCAGCTGAG CTGGGCAGAG 
CCAGCTGAGA CCAATGGCGA GATCACAGCC TACGAGGTCT GCTATGGACT GGTCAATGAG 
GACAACCGAC CCATTGGACC TATGAAGAAG GTGCTCGTGG ACAACCCCAA GAACCGGATG 
CTGCTCATTG AGAATCTGCG AGAATCCCAG CCATACCGAT ACACGGTTAA GGCGCGCAAT 
GGGGCAGGAT GGGGACCCGA GAGAGAGGCT ATCATCAACC TGGCTACACA GCCCAAGCGG 
CCCATGTCCA TCCCTATCAT CCCAGACATC CCCATAGTGG ACGCCCAGGG TGGAGAAGAC 
TACGAAAACT TCCTTATGTA CAGTGATGAC GTCCTGCGGT CCCCAGCCAG CAGCCAGAGG 
CCCAGCGTTT CTGATGACAC TGAGCACCTG GTGAATGGCC GGATGGACTT TGCCTATCCA 
GGCAGCGCCA ACTCCCTGCA CAGAATGACT GCAGCCAATG TGGCCTATGG CACGCATCTG 
AGCCCACACC TGTCCCACCG AGTGCTGAGC ACGTCCTCCA CCCTTACCCG GGACTACCAC 
TCTCTGACAC GCACAGAGCA CTCCCACTCA GGCACACTTC CCAGGGACTA CTCCACCCTC 
ACTTCCCTTT CCTCCCAAGG CCTCCCTCCT ATCTGGGAAG ATGGGAGGAG CAGGCTTCCG 
CTGTCCTGGA CTCTTGGGTC CTTGAGCCGG GCTCACATGA AGGGTGTGCC CGCATCCAGG 
GGTTCACCAG ACTCTATAAT CCTGGCCGGG CAGTCAGCAG CACCCTCCTG GGGTACAGAT 
TCCCGTGGGG CTGTGGGTGT GCCTGACACA CCCACTCGGC TGGTGTTCTC TGCCCTGGGG 
CCCACGTCTT TGAAGGTGAG CTGGCAGGAG CCACAGTGTG ATCGGATGCT GCTGGGCTAC 
AGTGTGGAAT ACCAGCTACT AAACGGCGGC GAGATGCATC GGCTCAACAT CCCTAACCCT 
GGCCAAACCT CGGTGGTGGT AGAGGATCTC CTGCCTAACC ACTCCTATGT GTTCCGGGTA 
CGGGCCCAGA GCCAGGAGGG CTGGGGCCGA GAGCGAGAGG GTGTCATCAC CATCGAGTCC 
CAGGTGCACC CGCAGAGCCC TCTCTGCCCC CTGCCAGGCT CAGCCTTCAC TCTGAGCACC 
CCCAGCGCCC CGGGACCGCT GGTGTTCACT GCCCTAAGCC CAGACTCCCT GCAGCTCAGC 
TGGGAGCGGC CGAGGAGGCC CAATGGAGAT ATCCTTGGCT ACCTGGTGAC CTGTGAGATG 
GCCCAAGGAG GAGCACCAGC CAGGACCTTC CGGGTGGACG GAGACAACCC TGAGAGCCGG 
TTGACTGTAC CTGGCCTCAG TGAGAACGTT CCTTACAAGT TCAAGGTTCA GGCCAGGACG 
ACCGAGGGCT TCGGGCCAGA GCGTGAGGGT ATCATCACCA TCGAGTCTCA GGTTGGAGGC 
CCCTTCCCAC AGCTGGGCAG CCATTCTGGG CTCTTCCAGA ACCCAGTGCA AAGCGAGTTC 
AGCAGCGTGA CCAGCACGCA CAGCACCACG ACTGAGCCCT TCCTCATGGA TGGTCTAACC 
CTGGGGACCC AGCGCCTGGA AGCAGGAGGC TCCCTCACCC GGCATGTGAC CCAGGAATTC 
GTGACCCGGA CCTTAACGGC CAGTGGCTCT CTCAGCACTC ATATGGACCA ACAGTTCTTC 
CAAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005608.2
CDS	441..5849
Translation	MAGPCCSPWVKLLLLAAMLSASLPGDLANRCKKAQVKSCTECIRVDKSCAYCTDELFKERRCNTQAELLAAGCRGESILVMESSLEITENTQIDTSLHRSQVSPQGLQVRLRPGEERSFVFQVFEPLESPVDLYILMDFSNSMSDDLDNLKQMGQNLAKILRQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTENVEEFWNKLQGERISGNLDAPEGGFDAILQTAVCTRDIGWRADSTHLLVFSTESAFHYEADGANVLAGIMNRNDEKCHLDASGAYTQYKTQDYPSVPTLVRLLAKHNIIPIFAVTNYSYSYYEKLHKYFPVSSLGVLQEDSSNIVELLEEAFYRIRSNLDIRALDSPRGLRTEVTSDTLQKTETGSFHIKRGEVGTYNVHLRAVEDIDGTHVCQLAKEDQGGNIHLKPSFSDGLRMDASVICDVCPCELQKEVRSARCHFRGDFMCGHCVCNEGWSGKTCNCSTGSLSDTQPCLREGEDKPCSGHGECQCGRCVCYGEGRYEGHFCEYDNFQCPRTSGFLCNDRGRCSMGECVCEPGWTGRSCDCPLSNATCIDSNGGICNGRGYCECGRCHCNQQSLYTDTTCEINYSAIRLGLCEDLRSCVQCQAWGTGEKKGRACDDCPFKVKMVDELKKEEVVEYCSFRDEDDDCTYSYNVEGDGSPGPNSTVLVHKKKDCPPGSFWWLIPLLIFLLLLLALLLLLCWKYCACCKACLGLLPCCNRGHMVGFKEDHYMLRENLMASDHLDTPMLRSGNLKGRDTVRWKITNNVQRPGFATHAASTSPTELVPYGLSLRLGRLCTENLMKPGTRECDQLRQEVEENLNEVYRQVSGAHKLQQTKFRQQPNTGKKQDHTIVDTVLLAPRSAKQMLLKLTEKQVEQGSFHELKVAPGYYTVTAEQDARGMVEFQEGVELVDVRVPLFIRPEDDDEKQLLVEAIDVPVGTATLGRRLVNITIIKEQASGVVSFEQPEYSVSRGDQVARIPVIRHILDNGKSQVSYSTQDNTAHGHRDYVPVEGELLFHPGETWKELQVKLLELQEVDSLLRGRQVRRFQVQLSNPKFGARLGQPSTTTVILDETDRSLINQTLSSPPPPHGDLGAPQNPNAKAAGSRKIHFNWLPPPGKPMGYRVKYWIQGDSESEAHLLDSKVPSVELTNLYPYCDYEMKVCAYGAQGEGPYSSLVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYGLVNEDNRPIGPMKKVLVDNPKNRMLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDIPIVDAQGGEDYENFLMYSDDVLRSPASSQRPSVSDDTEHLVNGRMDFAYPGSANSLHRMTAANVAYGTHLSPHLSHRVLSTSSTLTRDYHSLTRTEHSHSGTLPRDYSTLTSLSSQGLPPIWEDGRSRLPLSWTLGSLSRAHMKGVPASRGSPDSIILAGQSAAPSWGTDSRGAVGVPDTPTRLVFSALGPTSLKVSWQEPQCDRMLLGYSVEYQLLNGGEMHRLNIPNPGQTSVVVEDLLPNHSYVFRVRAQSQEGWGREREGVITIESQVHPQSPLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPNGDILGYLVTCEMAQGGAPARTFRVDGDNPESRLTVPGLSENVPYKFKVQARTTEGFGPEREGIITIESQVGGPFPQLGSHSGLFQNPVQSEFSSVTSTHSTTTEPFLMDGLTLGTQRLEAGGSLTRHVTQEFVTRTLTASGSLSTHMDQQFFQT

Target ORF information:

RefSeq Version	NM_001005608.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus integrin beta 4 (Itgb4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005608.2

ORF Insert Sequence:

ATGGCAGGGC CCTGTTGCAG CCCATGGGTG AAGCTGCTGC TGCTGGCAGC AATGCTGAGT 
GCCAGCCTCC CTGGAGACCT GGCCAACCGC TGCAAGAAGG CTCAGGTGAA GAGCTGTACC 
GAGTGCATCC GGGTGGACAA GAGCTGTGCC TACTGCACAG ACGAGCTGTT CAAGGAGAGG 
CGCTGCAACA CCCAGGCGGA GCTTCTGGCT GCAGGCTGCA GGGGAGAGAG CATCCTGGTC 
ATGGAGAGCA GCCTTGAAAT CACAGAGAAC ACCCAGATCG ACACCAGCCT GCACCGCAGC 
CAGGTATCTC CCCAAGGCCT GCAAGTCCGG CTGCGGCCGG GTGAGGAGCG CAGCTTTGTG 
TTCCAGGTGT TTGAGCCCCT GGAGAGCCCC GTGGATCTGT ATATCCTCAT GGACTTCTCC 
AACTCCATGT CTGACGATCT GGACAACCTC AAGCAGATGG GGCAGAACCT GGCCAAGATC 
CTGCGCCAGC TCACCAGCGA CTACACCATT GGATTTGGAA AGTTTGTGGA CAAAGTCAGC 
GTCCCACAGA CAGACATGAG GCCCGAGAAA CTGAAGGAGC CCTGGCCCAA CAGTGATCCC 
CCGTTCTCCT TCAAGAACGT TATCAGCTTA ACGGAGAATG TGGAAGAATT CTGGAACAAA 
CTGCAAGGAG AACGCATCTC AGGCAACCTG GACGCTCCTG AAGGGGGCTT TGATGCCATC 
CTGCAGACAG CTGTGTGCAC AAGGGACATT GGCTGGAGGG CTGACAGCAC CCACCTGCTG 
GTGTTCTCCA CCGAGTCTGC CTTCCACTAC GAGGCTGATG GTGCCAACGT GCTGGCCGGC 
ATCATGAACC GCAATGATGA GAAATGCCAC CTGGACGCCT CGGGCGCCTA CACCCAATAC 
AAGACACAGG ACTACCCATC AGTGCCCACG CTGGTTCGCC TGCTTGCCAA GCATAACATC 
ATCCCCATCT TTGCTGTCAC CAACTACTCT TACAGCTACT ATGAGAAGCT CCATAAGTAT 
TTCCCCGTCT CCTCTCTGGG CGTCCTGCAG GAGGATTCAT CCAACATCGT GGAGCTGCTG 
GAGGAGGCCT TCTATCGAAT TCGCTCCAAC CTGGACATCC GGGCTCTGGA CAGCCCCAGA 
GGCCTGAGAA CAGAGGTCAC CTCCGATACT CTCCAGAAGA CGGAGACTGG GTCCTTTCAC 
ATCAAGCGGG GGGAAGTGGG CACATACAAT GTGCATCTCC GGGCAGTGGA GGACATAGAT 
GGGACACATG TGTGCCAGCT GGCTAAAGAA GACCAAGGGG GCAACATCCA CCTGAAACCC 
TCCTTCTCTG ATGGCCTCCG GATGGACGCG AGTGTGATCT GTGACGTGTG CCCCTGTGAG 
CTGCAAAAGG AAGTTCGATC AGCTCGCTGT CACTTCAGAG GAGACTTCAT GTGTGGACAC 
TGTGTGTGCA ATGAGGGCTG GAGTGGCAAA ACCTGCAACT GCTCCACCGG CTCTCTGAGT 
GACACACAGC CCTGCCTGCG TGAGGGTGAG GACAAACCGT GCTCGGGCCA CGGCGAGTGC 
CAGTGCGGAC GCTGTGTGTG CTATGGTGAA GGCCGCTACG AGGGTCACTT CTGCGAGTAT 
GACAACTTCC AGTGTCCCCG GACCTCTGGA TTCCTGTGCA ATGACCGGGG TCGCTGTTCT 
ATGGGAGAGT GTGTGTGTGA GCCTGGTTGG ACAGGCCGCA GCTGTGACTG TCCCCTCAGC 
AATGCCACCT GCATCGATAG CAACGGGGGC ATCTGCAACG GCCGAGGCTA CTGTGAGTGT 
GGCCGTTGTC ACTGCAACCA GCAGTCGCTC TACACGGACA CCACCTGTGA GATCAACTAC 
TCTGCGATAC GCCTGGGTCT CTGTGAGGAT CTCCGCTCCT GCGTACAGTG CCAGGCCTGG 
GGCACCGGGG AGAAGAAAGG GCGCGCGTGT GACGATTGCC CCTTTAAAGT CAAGATGGTA 
GACGAGCTTA AGAAAGAAGA GGTGGTGGAG TACTGCTCCT TCCGGGATGA GGATGACGAC 
TGCACTTACA GCTACAACGT GGAGGGCGAT GGCAGCCCTG GGCCCAACAG CACAGTCCTG 
GTCCACAAAA AGAAAGACTG CCCTCCCGGC TCCTTCTGGT GGCTCATCCC CCTGCTCATC 
TTCCTCCTGT TGCTCCTGGC GTTGCTTCTG CTGCTCTGCT GGAAATACTG TGCCTGCTGC 
AAAGCCTGCC TGGGGCTTCT TCCTTGCTGC AACCGAGGTC ACATGGTGGG CTTTAAGGAA 
GATCACTATA TGCTTCGGGA GAACCTGATG GCCTCTGACC ACCTGGACAC GCCCATGCTA 
CGAAGCGGGA ACCTCAAGGG ACGAGACACA GTCCGCTGGA AGATCACCAA CAATGTGCAG 
CGCCCTGGCT TTGCCACCCA TGCCGCCAGC ACCAGCCCCA CGGAGCTCGT ACCCTACGGG 
CTGTCCCTGC GCCTTGGCCG CCTCTGCACT GAGAACCTTA TGAAGCCGGG CACCCGAGAG 
TGTGACCAGC TACGCCAGGA GGTGGAGGAA AATCTGAATG AGGTGTATAG ACAGGTCAGC 
GGCGCACACA AGCTCCAGCA GACGAAGTTC CGACAGCAGC CCAACACCGG GAAAAAGCAA 
GACCACACCA TTGTGGACAC AGTGTTGCTG GCGCCCCGCT CCGCCAAGCA GATGCTGCTG 
AAGCTGACAG AGAAGCAGGT GGAGCAGGGG TCCTTCCATG AACTGAAGGT GGCCCCTGGC 
TACTACACTG TCACGGCAGA GCAGGATGCC CGGGGCATGG TGGAGTTCCA GGAGGGCGTG 
GAGCTGGTGG ATGTGCGAGT GCCCCTCTTC ATCCGGCCTG AGGATGATGA TGAGAAGCAG 
CTGCTGGTGG AGGCCATTGA TGTCCCTGTG GGCACTGCCA CCCTTGGTCG CCGTCTGGTA 
AACATCACCA TTATCAAGGA ACAAGCTAGT GGGGTAGTGT CCTTCGAGCA GCCTGAATAC 
TCGGTGAGTC GTGGAGACCA GGTGGCCCGC ATCCCTGTCA TCCGGCACAT CCTGGACAAT 
GGCAAGTCCC AGGTCTCCTA TAGCACACAG GATAATACAG CACACGGACA CCGGGATTAT 
GTCCCTGTGG AGGGAGAGCT GCTGTTCCAT CCTGGGGAGA CCTGGAAGGA GTTGCAGGTG 
AAGCTACTGG AGCTGCAGGA GGTCGACTCC CTCCTGCGTG GCCGCCAGGT CCGCCGCTTC 
CAAGTCCAAC TCAGCAACCC CAAGTTCGGA GCCCGCCTGG GCCAGCCCAG CACAACCACC 
GTTATTCTCG ATGAAACGGA CAGGAGTCTC ATAAATCAAA CACTTTCATC GCCTCCGCCA 
CCCCATGGAG ACCTGGGCGC CCCACAGAAC CCCAATGCCA AGGCTGCCGG GTCCAGGAAG 
ATCCATTTCA ACTGGCTGCC CCCTCCTGGC AAGCCAATGG GGTACAGGGT GAAGTACTGG 
ATCCAGGGCG ACTCTGAGTC TGAAGCCCAC CTTCTAGACA GCAAGGTGCC CTCAGTGGAA 
CTCACCAACC TGTATCCCTA TTGCGACTAC GAAATGAAGG TGTGTGCCTA TGGGGCACAG 
GGTGAGGGGC CCTATAGCTC ACTGGTGTCC TGCCGCACCC ACCAGGAAGT ACCCAGCGAG 
CCAGGGAGGC TGGCTTTCAA TGTAGTCTCT TCTACGGTGA CGCAGCTGAG CTGGGCAGAG 
CCAGCTGAGA CCAATGGCGA GATCACAGCC TACGAGGTCT GCTATGGACT GGTCAATGAG 
GACAACCGAC CCATTGGACC TATGAAGAAG GTGCTCGTGG ACAACCCCAA GAACCGGATG 
CTGCTCATTG AGAATCTGCG AGAATCCCAG CCATACCGAT ACACGGTTAA GGCGCGCAAT 
GGGGCAGGAT GGGGACCCGA GAGAGAGGCT ATCATCAACC TGGCTACACA GCCCAAGCGG 
CCCATGTCCA TCCCTATCAT CCCAGACATC CCCATAGTGG ACGCCCAGGG TGGAGAAGAC 
TACGAAAACT TCCTTATGTA CAGTGATGAC GTCCTGCGGT CCCCAGCCAG CAGCCAGAGG 
CCCAGCGTTT CTGATGACAC TGAGCACCTG GTGAATGGCC GGATGGACTT TGCCTATCCA 
GGCAGCGCCA ACTCCCTGCA CAGAATGACT GCAGCCAATG TGGCCTATGG CACGCATCTG 
AGCCCACACC TGTCCCACCG AGTGCTGAGC ACGTCCTCCA CCCTTACCCG GGACTACCAC 
TCTCTGACAC GCACAGAGCA CTCCCACTCA GGCACACTTC CCAGGGACTA CTCCACCCTC 
ACTTCCCTTT CCTCCCAAGG CCTCCCTCCT ATCTGGGAAG ATGGGAGGAG CAGGCTTCCG 
CTGTCCTGGA CTCTTGGGTC CTTGAGCCGG GCTCACATGA AGGGTGTGCC CGCATCCAGG 
GGTTCACCAG ACTCTATAAT CCTGGCCGGG CAGTCAGCAG CACCCTCCTG GGGTACAGAT 
TCCCGTGGGG CTGTGGGTGT GCCTGACACA CCCACTCGGC TGGTGTTCTC TGCCCTGGGG 
CCCACGTCTT TGAAGGTGAG CTGGCAGGAG CCACAGTGTG ATCGGATGCT GCTGGGCTAC 
AGTGTGGAAT ACCAGCTACT AAACGGCGGC GAGATGCATC GGCTCAACAT CCCTAACCCT 
GGCCAAACCT CGGTGGTGGT AGAGGATCTC CTGCCTAACC ACTCCTATGT GTTCCGGGTA 
CGGGCCCAGA GCCAGGAGGG CTGGGGCCGA GAGCGAGAGG GTGTCATCAC CATCGAGTCC 
CAGGTGCACC CGCAGAGCCC TCTCTGCCCC CTGCCAGGCT CAGCCTTCAC TCTGAGCACC 
CCCAGCGCCC CGGGACCGCT GGTGTTCACT GCCCTAAGCC CAGACTCCCT GCAGCTCAGC 
TGGGAGCGGC CGAGGAGGCC CAATGGAGAT ATCCTTGGCT ACCTGGTGAC CTGTGAGATG 
GCCCAAGGAG GAGCACCAGC CAGGACCTTC CGGGTGGACG GAGACAACCC TGAGAGCCGG 
TTGACTGTAC CTGGCCTCAG TGAGAACGTT CCTTACAAGT TCAAGGTTCA GGCCAGGACG 
ACCGAGGGCT TCGGGCCAGA GCGTGAGGGT ATCATCACCA TCGAGTCTCA GGTTGGAGGC 
CCCTTCCCAC AGCTGGGCAG CCATTCTGGG CTCTTCCAGA ACCCAGTGCA AAGCGAGTTC 
AGCAGCGTGA CCAGCACGCA CAGCACCACG ACTGAGCCCT TCCTCATGGA TGGTCTAACC 
CTGGGGACCC AGCGCCTGGA AGCAGGAGGC TCCCTCACCC GGCATGTGAC CCAGGAATTC 
GTGACCCGGA CCTTAACGGC CAGTGGCTCT CTCAGCACTC ATATGGACCA ACAGTTCTTC 
CAAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itgb4 ( XM_006532572.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532635.1 Mus musculus integrin beta 4 (Itgb4), transcript variant X4, mRNA.

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CloneID	OMu45808
Clone ID Related Accession (Same CDS sequence)	XM_006532572.4 , XM_006532572.3
Accession Version	XM_006532572.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	integrin beta-4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532572.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGC CCTGTTGCAG CCCATGGGTG AAGCTGCTGC TGCTGGCAGC AATGCTGAGT 
GCCAGCCTCC CTGGAGACCT GGCCAACCGC TGCAAGAAGG CTCAGGTGAA GAGCTGTACC 
GAGTGCATCC GGGTGGACAA GAGCTGTGCC TACTGCACAG ACGAGCTGTT CAAGGAGAGG 
CGCTGCAACA CCCAGGCGGA GCTTCTGGCT GCAGGCTGCA GGGGAGAGAG CATCCTGGTC 
ATGGAGAGCA GCCTTGAAAT CACAGAGAAC ACCCAGATCG ACACCAGCCT GCACCGCAGC 
CAGGTATCTC CCCAAGGCCT GCAAGTCCGG CTGCGGCCGG GTGAGGAGCG CAGCTTTGTG 
TTCCAGGTGT TTGAGCCCCT GGAGAGCCCC GTGGATCTGT ATATCCTCAT GGACTTCTCC 
AACTCCATGT CTGACGATCT GGACAACCTC AAGCAGATGG GGCAGAACCT GGCCAAGATC 
CTGCGCCAGC TCACCAGCGA CTACACCATT GGATTTGGAA AGTTTGTGGA CAAAGTCAGC 
GTCCCACAGA CAGACATGAG GCCCGAGAAA CTGAAGGAGC CCTGGCCCAA CAGTGATCCC 
CCGTTCTCCT TCAAGAACGT TATCAGCTTA ACGGAGAATG TGGAAGAATT CTGGAACAAA 
CTGCAAGGAG AACGCATCTC AGGCAACCTG GACGCTCCTG AAGGGGGCTT TGATGCCATC 
CTGCAGACAG CTGTGTGCAC AAGGGACATT GGCTGGAGGG CTGACAGCAC CCACCTGCTG 
GTGTTCTCCA CCGAGTCTGC CTTCCACTAC GAGGCTGATG GTGCCAACGT GCTGGCCGGC 
ATCATGAACC GCAATGATGA GAAATGCCAC CTGGACGCCT CGGGCGCCTA CACCCAATAC 
AAGACACAGG ACTACCCATC AGTGCCCACG CTGGTTCGCC TGCTTGCCAA GCATAACATC 
ATCCCCATCT TTGCTGTCAC CAACTACTCT TACAGCTACT ATGAGAAGCT CCATAAGTAT 
TTCCCCGTCT CCTCTCTGGG CGTCCTGCAG GAGGATTCAT CCAACATCGT GGAGCTGCTG 
GAGGAGGCCT TCTATCGAAT TCGCTCCAAC CTGGACATCC GGGCTCTGGA CAGCCCCAGA 
GGCCTGAGAA CAGAGGTCAC CTCCGATACT CTCCAGAAGA CGGAGACTGG GTCCTTTCAC 
ATCAAGCGGG GGGAAGTGGG CACATACAAT GTGCATCTCC GGGCAGTGGA GGACATAGAT 
GGGACACATG TGTGCCAGCT GGCTAAAGAA GACCAAGGGG GCAACATCCA CCTGAAACCC 
TCCTTCTCTG ATGGCCTCCG GATGGACGCG AGTGTGATCT GTGACGTGTG CCCCTGTGAG 
CTGCAAAAGG AAGTTCGATC AGCTCGCTGT CACTTCAGAG GAGACTTCAT GTGTGGACAC 
TGTGTGTGCA ATGAGGGCTG GAGTGGCAAA ACCTGCAACT GCTCCACCGG CTCTCTGAGT 
GACACACAGC CCTGCCTGCG TGAGGGTGAG GACAAACCGT GCTCGGGCCA CGGCGAGTGC 
CAGTGCGGAC GCTGTGTGTG CTATGGTGAA GGCCGCTACG AGGGTCACTT CTGCGAGTAT 
GACAACTTCC AGTGTCCCCG GACCTCTGGA TTCCTGTGCA ATGACCGGGG TCGCTGTTCT 
ATGGGAGAGT GTGTGTGTGA GCCTGGTTGG ACAGGCCGCA GCTGTGACTG TCCCCTCAGC 
AATGCCACCT GCATCGATAG CAACGGGGGC ATCTGCAACG GCCGAGGCTA CTGTGAGTGT 
GGCCGTTGTC ACTGCAACCA GCAGTCGCTC TACACGGACA CCACCTGTGA GATCAACTAC 
TCTGCGATAC GCCTGGGTCT CTGTGAGGAT CTCCGCTCCT GCGTACAGTG CCAGGCCTGG 
GGCACCGGGG AGAAGAAAGG GCGCGCGTGT GACGATTGCC CCTTTAAAGT CAAGATGGTA 
GACGAGCTTA AGAAAGCAGA AGAGGTGGTG GAGTACTGCT CCTTCCGGGA TGAGGATGAC 
GACTGCACTT ACAGCTACAA CGTGGAGGGC GATGGCAGCC CTGGGCCCAA CAGCACAGTC 
CTGGTCCACA AAAAGAAAGA CTGCCCTCCC GGCTCCTTCT GGTGGCTCAT CCCCCTGCTC 
ATCTTCCTCC TGTTGCTCCT GGCGTTGCTT CTGCTGCTCT GCTGGAAATA CTGTGCCTGC 
TGCAAAGCCT GCCTGGGGCT TCTTCCTTGC TGCAACCGAG GTCACATGGT GGGCTTTAAG 
GAAGATCACT ATATGCTTCG GGAGAACCTG ATGGCCTCTG ACCACCTGGA CACGCCCATG 
CTACGAAGCG GGAACCTCAA GGGACGAGAC ACAGTCCGCT GGAAGATCAC CAACAATGTG 
CAGCGCCCTG GCTTTGCCAC CCATGCCGCC AGCACCAGCC CCACGGAGCT CGTACCCTAC 
GGGCTGTCCC TGCGCCTTGG CCGCCTCTGC ACTGAGAACC TTATGAAGCC GGGCACCCGA 
GAGTGTGACC AGCTACGCCA GGAGGTGGAG GAAAATCTGA ATGAGGTGTA TAGACAGGTC 
AGCGGCGCAC ACAAGCTCCA GCAGACGAAG TTCCGACAGC AGCCCAACAC CGGGAAAAAG 
CAAGACCACA CCATTGTGGA CACAGTGTTG CTGGCGCCCC GCTCCGCCAA GCAGATGCTG 
CTGAAGCTGA CAGAGAAGCA GGTGGAGCAG GGGTCCTTCC ATGAACTGAA GGTGGCCCCT 
GGCTACTACA CTGTCACGGC AGAGCAGGAT GCCCGGGGCA TGGTGGAGTT CCAGGAGGGC 
GTGGAGCTGG TGGATGTGCG AGTGCCCCTC TTCATCCGGC CTGAGGATGA TGATGAGAAG 
CAGCTGCTGG TGGAGGCCAT TGATGTCCCT GTGGGCACTG CCACCCTTGG TCGCCGTCTG 
GTAAACATCA CCATTATCAA GGAACAAGCT AGTGGGGTAG TGTCCTTCGA GCAGCCTGAA 
TACTCGGTGA GTCGTGGAGA CCAGGTGGCC CGCATCCCTG TCATCCGGCA CATCCTGGAC 
AATGGCAAGT CCCAGGTCTC CTATAGCACA CAGGATAATA CAGCACACGG ACACCGGGAT 
TATGTCCCTG TGGAGGGAGA GCTGCTGTTC CATCCTGGGG AGACCTGGAA GGAGTTGCAG 
GTGAAGCTAC TGGAGCTGCA GGAGGTCGAC TCCCTCCTGC GTGGCCGCCA GGTCCGCCGC 
TTCCAAGTCC AACTCAGCAA CCCCAAGTTC GGAGCCCGCC TGGGCCAGCC CAGCACAACC 
ACCGTTATTC TCGGTGAGCA CGATGAAACG GACAGGAGTC TCATAAATCA AACACTTTCA 
TCGCCTCCGC CACCCCATGG AGACCTGGGC GCCCCACAGA ACCCCAATGC CAAGGCTGCC 
GGGTCCAGGA AGATCCATTT CAACTGGCTG CCCCCTCCTG GCAAGCCAAT GGGGTACAGG 
GTGAAGTACT GGATCCAGGG CGACTCTGAG TCTGAAGCCC ACCTTCTAGA CAGCAAGGTG 
CCCTCAGTGG AACTCACCAA CCTGTATCCC TATTGCGACT ACGAAATGAA GGTGTGTGCC 
TATGGGGCAC AGGGTGAGGG GCCCTATAGC TCACTGGTGT CCTGCCGCAC CCACCAGGAA 
GTACCCAGCG AGCCAGGGAG GCTGGCTTTC AATGTAGTCT CTTCTACGGT GACGCAGCTG 
AGCTGGGCAG AGCCAGCTGA GACCAATGGC GAGATCACAG CCTACGAGGT CTGCTATGGA 
CTGGTCAATG AGGACAACCG ACCCATTGGA CCTATGAAGA AGGTGCTCGT GGACAACCCC 
AAGAACCGGA TGCTGCTCAT TGAGAATCTG CGAGAATCCC AGCCATACCG ATACACGGTT 
AAGGCGCGCA ATGGGGCAGG ATGGGGACCC GAGAGAGAGG CTATCATCAA CCTGGCTACA 
CAGCCCAAGC GGCCCATGTC CATCCCTATC ATCCCAGACA TCCCCATAGT GGACGCCCAG 
GGTGGAGAAG ACTACGAAAA CTTCCTTATG TACAGTGATG ACGTCCTGCG GTCCCCAGCC 
AGCAGCCAGA GGCCCAGCGT TTCTGATGAC ACTGGCTGCG GCTGGAAGTT CGAGCCTCTG 
CTCGGGGAGG AGCTGGACCT GCGGCGCGTC ACGTGGCGGC TACCCCCGGA ACTCATCCCG 
CGCCTGTCCG CCAGCAGCGG GCGCTCTGAC GAAGACGGCA GCGTTGCGGG GGGCGTGGAA 
GGGGAGGGCA GCGGCTGGAT CCGCGGGGCT ACGCCCCGAC CCCCGGGAGA GCACCTGGTG 
AATGGCCGGA TGGACTTTGC CTATCCAGGC AGCGCCAACT CCCTGCACAG AATGACTGCA 
GCCAATGTGG CCTATGGCAC GCATCTGAGC CCACACCTGT CCCACCGAGT GCTGAGCACG 
TCCTCCACCC TTACCCGGGA CTACCACTCT CTGACACGCA CAGAGCACTC CCACTCAGGC 
ACACTTCCCA GGGACTACTC CACCCTCACT TCCCTTTCCT CCCAAGATTC CCGTGGGGCT 
GTGGGTGTGC CTGACACACC CACTCGGCTG GTGTTCTCTG CCCTGGGGCC CACGTCTTTG 
AAGGTGAGCT GGCAGGAGCC ACAGTGTGAT CGGATGCTGC TGGGCTACAG TGTGGAATAC 
CAGCTACTAA ACGGCGGCGA GATGCATCGG CTCAACATCC CTAACCCTGG CCAAACCTCG 
GTGGTGGTAG AGGATCTCCT GCCTAACCAC TCCTATGTGT TCCGGGTACG GGCCCAGAGC 
CAGGAGGGCT GGGGCCGAGA GCGAGAGGGT GTCATCACCA TCGAGTCCCA GGTGCACCCG 
CAGAGCCCTC TCTGCCCCCT GCCAGGCTCA GCCTTCACTC TGAGCACCCC CAGCGCCCCG 
GGACCGCTGG TGTTCACTGC CCTAAGCCCA GACTCCCTGC AGCTCAGCTG GGAGCGGCCG 
AGGAGGCCCA ATGGAGATAT CCTTGGCTAC CTGGTGACCT GTGAGATGGC CCAAGGAGGA 
GCACCAGCCA GGACCTTCCG GGTGGACGGA GACAACCCTG AGAGCCGGTT GACTGTACCT 
GGCCTCAGTG AGAACGTTCC TTACAAGTTC AAGGTTCAGG CCAGGACGAC CGAGGGCTTC 
GGGCCAGAGC GTGAGGGTAT CATCACCATC GAGTCTCAGG TTGGAGGCCC CTTCCCACAG 
CTGGGCAGCC ATTCTGGGCT CTTCCAGAAC CCAGTGCAAA GCGAGTTCAG CAGCGTGACC 
AGCACGCACA GCACCACGAC TGAGCCCTTC CTCATGGATG GTCTAACCCT GGGGACCCAG 
CGCCTGGAAG CAGGAGGCTC CCTCACCCGG CATGTGACCC AGGAATTCGT GACCCGGACC 
TTAACGGCCA GTGGCTCTCT CAGCACTCAT ATGGACCAAC AGTTCTTCCA AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532635.1
CDS	436..5892
Translation	MAGPCCSPWVKLLLLAAMLSASLPGDLANRCKKAQVKSCTECIRVDKSCAYCTDELFKERRCNTQAELLAAGCRGESILVMESSLEITENTQIDTSLHRSQVSPQGLQVRLRPGEERSFVFQVFEPLESPVDLYILMDFSNSMSDDLDNLKQMGQNLAKILRQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTENVEEFWNKLQGERISGNLDAPEGGFDAILQTAVCTRDIGWRADSTHLLVFSTESAFHYEADGANVLAGIMNRNDEKCHLDASGAYTQYKTQDYPSVPTLVRLLAKHNIIPIFAVTNYSYSYYEKLHKYFPVSSLGVLQEDSSNIVELLEEAFYRIRSNLDIRALDSPRGLRTEVTSDTLQKTETGSFHIKRGEVGTYNVHLRAVEDIDGTHVCQLAKEDQGGNIHLKPSFSDGLRMDASVICDVCPCELQKEVRSARCHFRGDFMCGHCVCNEGWSGKTCNCSTGSLSDTQPCLREGEDKPCSGHGECQCGRCVCYGEGRYEGHFCEYDNFQCPRTSGFLCNDRGRCSMGECVCEPGWTGRSCDCPLSNATCIDSNGGICNGRGYCECGRCHCNQQSLYTDTTCEINYSAIRLGLCEDLRSCVQCQAWGTGEKKGRACDDCPFKVKMVDELKKAEEVVEYCSFRDEDDDCTYSYNVEGDGSPGPNSTVLVHKKKDCPPGSFWWLIPLLIFLLLLLALLLLLCWKYCACCKACLGLLPCCNRGHMVGFKEDHYMLRENLMASDHLDTPMLRSGNLKGRDTVRWKITNNVQRPGFATHAASTSPTELVPYGLSLRLGRLCTENLMKPGTRECDQLRQEVEENLNEVYRQVSGAHKLQQTKFRQQPNTGKKQDHTIVDTVLLAPRSAKQMLLKLTEKQVEQGSFHELKVAPGYYTVTAEQDARGMVEFQEGVELVDVRVPLFIRPEDDDEKQLLVEAIDVPVGTATLGRRLVNITIIKEQASGVVSFEQPEYSVSRGDQVARIPVIRHILDNGKSQVSYSTQDNTAHGHRDYVPVEGELLFHPGETWKELQVKLLELQEVDSLLRGRQVRRFQVQLSNPKFGARLGQPSTTTVILGEHDETDRSLINQTLSSPPPPHGDLGAPQNPNAKAAGSRKIHFNWLPPPGKPMGYRVKYWIQGDSESEAHLLDSKVPSVELTNLYPYCDYEMKVCAYGAQGEGPYSSLVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYGLVNEDNRPIGPMKKVLVDNPKNRMLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDIPIVDAQGGEDYENFLMYSDDVLRSPASSQRPSVSDDTGCGWKFEPLLGEELDLRRVTWRLPPELIPRLSASSGRSDEDGSVAGGVEGEGSGWIRGATPRPPGEHLVNGRMDFAYPGSANSLHRMTAANVAYGTHLSPHLSHRVLSTSSTLTRDYHSLTRTEHSHSGTLPRDYSTLTSLSSQDSRGAVGVPDTPTRLVFSALGPTSLKVSWQEPQCDRMLLGYSVEYQLLNGGEMHRLNIPNPGQTSVVVEDLLPNHSYVFRVRAQSQEGWGREREGVITIESQVHPQSPLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPNGDILGYLVTCEMAQGGAPARTFRVDGDNPESRLTVPGLSENVPYKFKVQARTTEGFGPEREGIITIESQVGGPFPQLGSHSGLFQNPVQSEFSSVTSTHSTTTEPFLMDGLTLGTQRLEAGGSLTRHVTQEFVTRTLTASGSLSTHMDQQFFQT

Target ORF information:

RefSeq Version	XM_006532572.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus integrin beta 4 (Itgb4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532572.3

ORF Insert Sequence:

ATGGCAGGGC CCTGTTGCAG CCCATGGGTG AAGCTGCTGC TGCTGGCAGC AATGCTGAGT 
GCCAGCCTCC CTGGAGACCT GGCCAACCGC TGCAAGAAGG CTCAGGTGAA GAGCTGTACC 
GAGTGCATCC GGGTGGACAA GAGCTGTGCC TACTGCACAG ACGAGCTGTT CAAGGAGAGG 
CGCTGCAACA CCCAGGCGGA GCTTCTGGCT GCAGGCTGCA GGGGAGAGAG CATCCTGGTC 
ATGGAGAGCA GCCTTGAAAT CACAGAGAAC ACCCAGATCG ACACCAGCCT GCACCGCAGC 
CAGGTATCTC CCCAAGGCCT GCAAGTCCGG CTGCGGCCGG GTGAGGAGCG CAGCTTTGTG 
TTCCAGGTGT TTGAGCCCCT GGAGAGCCCC GTGGATCTGT ATATCCTCAT GGACTTCTCC 
AACTCCATGT CTGACGATCT GGACAACCTC AAGCAGATGG GGCAGAACCT GGCCAAGATC 
CTGCGCCAGC TCACCAGCGA CTACACCATT GGATTTGGAA AGTTTGTGGA CAAAGTCAGC 
GTCCCACAGA CAGACATGAG GCCCGAGAAA CTGAAGGAGC CCTGGCCCAA CAGTGATCCC 
CCGTTCTCCT TCAAGAACGT TATCAGCTTA ACGGAGAATG TGGAAGAATT CTGGAACAAA 
CTGCAAGGAG AACGCATCTC AGGCAACCTG GACGCTCCTG AAGGGGGCTT TGATGCCATC 
CTGCAGACAG CTGTGTGCAC AAGGGACATT GGCTGGAGGG CTGACAGCAC CCACCTGCTG 
GTGTTCTCCA CCGAGTCTGC CTTCCACTAC GAGGCTGATG GTGCCAACGT GCTGGCCGGC 
ATCATGAACC GCAATGATGA GAAATGCCAC CTGGACGCCT CGGGCGCCTA CACCCAATAC 
AAGACACAGG ACTACCCATC AGTGCCCACG CTGGTTCGCC TGCTTGCCAA GCATAACATC 
ATCCCCATCT TTGCTGTCAC CAACTACTCT TACAGCTACT ATGAGAAGCT CCATAAGTAT 
TTCCCCGTCT CCTCTCTGGG CGTCCTGCAG GAGGATTCAT CCAACATCGT GGAGCTGCTG 
GAGGAGGCCT TCTATCGAAT TCGCTCCAAC CTGGACATCC GGGCTCTGGA CAGCCCCAGA 
GGCCTGAGAA CAGAGGTCAC CTCCGATACT CTCCAGAAGA CGGAGACTGG GTCCTTTCAC 
ATCAAGCGGG GGGAAGTGGG CACATACAAT GTGCATCTCC GGGCAGTGGA GGACATAGAT 
GGGACACATG TGTGCCAGCT GGCTAAAGAA GACCAAGGGG GCAACATCCA CCTGAAACCC 
TCCTTCTCTG ATGGCCTCCG GATGGACGCG AGTGTGATCT GTGACGTGTG CCCCTGTGAG 
CTGCAAAAGG AAGTTCGATC AGCTCGCTGT CACTTCAGAG GAGACTTCAT GTGTGGACAC 
TGTGTGTGCA ATGAGGGCTG GAGTGGCAAA ACCTGCAACT GCTCCACCGG CTCTCTGAGT 
GACACACAGC CCTGCCTGCG TGAGGGTGAG GACAAACCGT GCTCGGGCCA CGGCGAGTGC 
CAGTGCGGAC GCTGTGTGTG CTATGGTGAA GGCCGCTACG AGGGTCACTT CTGCGAGTAT 
GACAACTTCC AGTGTCCCCG GACCTCTGGA TTCCTGTGCA ATGACCGGGG TCGCTGTTCT 
ATGGGAGAGT GTGTGTGTGA GCCTGGTTGG ACAGGCCGCA GCTGTGACTG TCCCCTCAGC 
AATGCCACCT GCATCGATAG CAACGGGGGC ATCTGCAACG GCCGAGGCTA CTGTGAGTGT 
GGCCGTTGTC ACTGCAACCA GCAGTCGCTC TACACGGACA CCACCTGTGA GATCAACTAC 
TCTGCGATAC GCCTGGGTCT CTGTGAGGAT CTCCGCTCCT GCGTACAGTG CCAGGCCTGG 
GGCACCGGGG AGAAGAAAGG GCGCGCGTGT GACGATTGCC CCTTTAAAGT CAAGATGGTA 
GACGAGCTTA AGAAAGCAGA AGAGGTGGTG GAGTACTGCT CCTTCCGGGA TGAGGATGAC 
GACTGCACTT ACAGCTACAA CGTGGAGGGC GATGGCAGCC CTGGGCCCAA CAGCACAGTC 
CTGGTCCACA AAAAGAAAGA CTGCCCTCCC GGCTCCTTCT GGTGGCTCAT CCCCCTGCTC 
ATCTTCCTCC TGTTGCTCCT GGCGTTGCTT CTGCTGCTCT GCTGGAAATA CTGTGCCTGC 
TGCAAAGCCT GCCTGGGGCT TCTTCCTTGC TGCAACCGAG GTCACATGGT GGGCTTTAAG 
GAAGATCACT ATATGCTTCG GGAGAACCTG ATGGCCTCTG ACCACCTGGA CACGCCCATG 
CTACGAAGCG GGAACCTCAA GGGACGAGAC ACAGTCCGCT GGAAGATCAC CAACAATGTG 
CAGCGCCCTG GCTTTGCCAC CCATGCCGCC AGCACCAGCC CCACGGAGCT CGTACCCTAC 
GGGCTGTCCC TGCGCCTTGG CCGCCTCTGC ACTGAGAACC TTATGAAGCC GGGCACCCGA 
GAGTGTGACC AGCTACGCCA GGAGGTGGAG GAAAATCTGA ATGAGGTGTA TAGACAGGTC 
AGCGGCGCAC ACAAGCTCCA GCAGACGAAG TTCCGACAGC AGCCCAACAC CGGGAAAAAG 
CAAGACCACA CCATTGTGGA CACAGTGTTG CTGGCGCCCC GCTCCGCCAA GCAGATGCTG 
CTGAAGCTGA CAGAGAAGCA GGTGGAGCAG GGGTCCTTCC ATGAACTGAA GGTGGCCCCT 
GGCTACTACA CTGTCACGGC AGAGCAGGAT GCCCGGGGCA TGGTGGAGTT CCAGGAGGGC 
GTGGAGCTGG TGGATGTGCG AGTGCCCCTC TTCATCCGGC CTGAGGATGA TGATGAGAAG 
CAGCTGCTGG TGGAGGCCAT TGATGTCCCT GTGGGCACTG CCACCCTTGG TCGCCGTCTG 
GTAAACATCA CCATTATCAA GGAACAAGCT AGTGGGGTAG TGTCCTTCGA GCAGCCTGAA 
TACTCGGTGA GTCGTGGAGA CCAGGTGGCC CGCATCCCTG TCATCCGGCA CATCCTGGAC 
AATGGCAAGT CCCAGGTCTC CTATAGCACA CAGGATAATA CAGCACACGG ACACCGGGAT 
TATGTCCCTG TGGAGGGAGA GCTGCTGTTC CATCCTGGGG AGACCTGGAA GGAGTTGCAG 
GTGAAGCTAC TGGAGCTGCA GGAGGTCGAC TCCCTCCTGC GTGGCCGCCA GGTCCGCCGC 
TTCCAAGTCC AACTCAGCAA CCCCAAGTTC GGAGCCCGCC TGGGCCAGCC CAGCACAACC 
ACCGTTATTC TCGGTGAGCA CGATGAAACG GACAGGAGTC TCATAAATCA AACACTTTCA 
TCGCCTCCGC CACCCCATGG AGACCTGGGC GCCCCACAGA ACCCCAATGC CAAGGCTGCC 
GGGTCCAGGA AGATCCATTT CAACTGGCTG CCCCCTCCTG GCAAGCCAAT GGGGTACAGG 
GTGAAGTACT GGATCCAGGG CGACTCTGAG TCTGAAGCCC ACCTTCTAGA CAGCAAGGTG 
CCCTCAGTGG AACTCACCAA CCTGTATCCC TATTGCGACT ACGAAATGAA GGTGTGTGCC 
TATGGGGCAC AGGGTGAGGG GCCCTATAGC TCACTGGTGT CCTGCCGCAC CCACCAGGAA 
GTACCCAGCG AGCCAGGGAG GCTGGCTTTC AATGTAGTCT CTTCTACGGT GACGCAGCTG 
AGCTGGGCAG AGCCAGCTGA GACCAATGGC GAGATCACAG CCTACGAGGT CTGCTATGGA 
CTGGTCAATG AGGACAACCG ACCCATTGGA CCTATGAAGA AGGTGCTCGT GGACAACCCC 
AAGAACCGGA TGCTGCTCAT TGAGAATCTG CGAGAATCCC AGCCATACCG ATACACGGTT 
AAGGCGCGCA ATGGGGCAGG ATGGGGACCC GAGAGAGAGG CTATCATCAA CCTGGCTACA 
CAGCCCAAGC GGCCCATGTC CATCCCTATC ATCCCAGACA TCCCCATAGT GGACGCCCAG 
GGTGGAGAAG ACTACGAAAA CTTCCTTATG TACAGTGATG ACGTCCTGCG GTCCCCAGCC 
AGCAGCCAGA GGCCCAGCGT TTCTGATGAC ACTGGCTGCG GCTGGAAGTT CGAGCCTCTG 
CTCGGGGAGG AGCTGGACCT GCGGCGCGTC ACGTGGCGGC TACCCCCGGA ACTCATCCCG 
CGCCTGTCCG CCAGCAGCGG GCGCTCTGAC GAAGACGGCA GCGTTGCGGG GGGCGTGGAA 
GGGGAGGGCA GCGGCTGGAT CCGCGGGGCT ACGCCCCGAC CCCCGGGAGA GCACCTGGTG 
AATGGCCGGA TGGACTTTGC CTATCCAGGC AGCGCCAACT CCCTGCACAG AATGACTGCA 
GCCAATGTGG CCTATGGCAC GCATCTGAGC CCACACCTGT CCCACCGAGT GCTGAGCACG 
TCCTCCACCC TTACCCGGGA CTACCACTCT CTGACACGCA CAGAGCACTC CCACTCAGGC 
ACACTTCCCA GGGACTACTC CACCCTCACT TCCCTTTCCT CCCAAGATTC CCGTGGGGCT 
GTGGGTGTGC CTGACACACC CACTCGGCTG GTGTTCTCTG CCCTGGGGCC CACGTCTTTG 
AAGGTGAGCT GGCAGGAGCC ACAGTGTGAT CGGATGCTGC TGGGCTACAG TGTGGAATAC 
CAGCTACTAA ACGGCGGCGA GATGCATCGG CTCAACATCC CTAACCCTGG CCAAACCTCG 
GTGGTGGTAG AGGATCTCCT GCCTAACCAC TCCTATGTGT TCCGGGTACG GGCCCAGAGC 
CAGGAGGGCT GGGGCCGAGA GCGAGAGGGT GTCATCACCA TCGAGTCCCA GGTGCACCCG 
CAGAGCCCTC TCTGCCCCCT GCCAGGCTCA GCCTTCACTC TGAGCACCCC CAGCGCCCCG 
GGACCGCTGG TGTTCACTGC CCTAAGCCCA GACTCCCTGC AGCTCAGCTG GGAGCGGCCG 
AGGAGGCCCA ATGGAGATAT CCTTGGCTAC CTGGTGACCT GTGAGATGGC CCAAGGAGGA 
GCACCAGCCA GGACCTTCCG GGTGGACGGA GACAACCCTG AGAGCCGGTT GACTGTACCT 
GGCCTCAGTG AGAACGTTCC TTACAAGTTC AAGGTTCAGG CCAGGACGAC CGAGGGCTTC 
GGGCCAGAGC GTGAGGGTAT CATCACCATC GAGTCTCAGG TTGGAGGCCC CTTCCCACAG 
CTGGGCAGCC ATTCTGGGCT CTTCCAGAAC CCAGTGCAAA GCGAGTTCAG CAGCGTGACC 
AGCACGCACA GCACCACGAC TGAGCCCTTC CTCATGGATG GTCTAACCCT GGGGACCCAG 
CGCCTGGAAG CAGGAGGCTC CCTCACCCGG CATGTGACCC AGGAATTCGT GACCCGGACC 
TTAACGGCCA GTGGCTCTCT CAGCACTCAT ATGGACCAAC AGTTCTTCCA AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itgb4 ( XM_006532573.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532636.1 Mus musculus integrin beta 4 (Itgb4), transcript variant X5, mRNA.

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CloneID	OMu45809
Clone ID Related Accession (Same CDS sequence)	XM_006532573.4 , XM_006532573.3
Accession Version	XM_006532573.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	integrin beta-4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532573.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGC CCTGTTGCAG CCCATGGGTG AAGCTGCTGC TGCTGGCAGC AATGCTGAGT 
GCCAGCCTCC CTGGAGACCT GGCCAACCGC TGCAAGAAGG CTCAGGTGAA GAGCTGTACC 
GAGTGCATCC GGGTGGACAA GAGCTGTGCC TACTGCACAG ACGAGCTGTT CAAGGAGAGG 
CGCTGCAACA CCCAGGCGGA GCTTCTGGCT GCAGGCTGCA GGGGAGAGAG CATCCTGGTC 
ATGGAGAGCA GCCTTGAAAT CACAGAGAAC ACCCAGATCG ACACCAGCCT GCACCGCAGC 
CAGGTATCTC CCCAAGGCCT GCAAGTCCGG CTGCGGCCGG GTGAGGAGCG CAGCTTTGTG 
TTCCAGGTGT TTGAGCCCCT GGAGAGCCCC GTGGATCTGT ATATCCTCAT GGACTTCTCC 
AACTCCATGT CTGACGATCT GGACAACCTC AAGCAGATGG GGCAGAACCT GGCCAAGATC 
CTGCGCCAGC TCACCAGCGA CTACACCATT GGATTTGGAA AGTTTGTGGA CAAAGTCAGC 
GTCCCACAGA CAGACATGAG GCCCGAGAAA CTGAAGGAGC CCTGGCCCAA CAGTGATCCC 
CCGTTCTCCT TCAAGAACGT TATCAGCTTA ACGGAGAATG TGGAAGAATT CTGGAACAAA 
CTGCAAGGAG AACGCATCTC AGGCAACCTG GACGCTCCTG AAGGGGGCTT TGATGCCATC 
CTGCAGACAG CTGTGTGCAC AAGGGACATT GGCTGGAGGG CTGACAGCAC CCACCTGCTG 
GTGTTCTCCA CCGAGTCTGC CTTCCACTAC GAGGCTGATG GTGCCAACGT GCTGGCCGGC 
ATCATGAACC GCAATGATGA GAAATGCCAC CTGGACGCCT CGGGCGCCTA CACCCAATAC 
AAGACACAGG ACTACCCATC AGTGCCCACG CTGGTTCGCC TGCTTGCCAA GCATAACATC 
ATCCCCATCT TTGCTGTCAC CAACTACTCT TACAGCTACT ATGAGAAGCT CCATAAGTAT 
TTCCCCGTCT CCTCTCTGGG CGTCCTGCAG GAGGATTCAT CCAACATCGT GGAGCTGCTG 
GAGGAGGCCT TCTATCGAAT TCGCTCCAAC CTGGACATCC GGGCTCTGGA CAGCCCCAGA 
GGCCTGAGAA CAGAGGTCAC CTCCGATACT CTCCAGAAGA CGGAGACTGG GTCCTTTCAC 
ATCAAGCGGG GGGAAGTGGG CACATACAAT GTGCATCTCC GGGCAGTGGA GGACATAGAT 
GGGACACATG TGTGCCAGCT GGCTAAAGAA GACCAAGGGG GCAACATCCA CCTGAAACCC 
TCCTTCTCTG ATGGCCTCCG GATGGACGCG AGTGTGATCT GTGACGTGTG CCCCTGTGAG 
CTGCAAAAGG AAGTTCGATC AGCTCGCTGT CACTTCAGAG GAGACTTCAT GTGTGGACAC 
TGTGTGTGCA ATGAGGGCTG GAGTGGCAAA ACCTGCAACT GCTCCACCGG CTCTCTGAGT 
GACACACAGC CCTGCCTGCG TGAGGGTGAG GACAAACCGT GCTCGGGCCA CGGCGAGTGC 
CAGTGCGGAC GCTGTGTGTG CTATGGTGAA GGCCGCTACG AGGGTCACTT CTGCGAGTAT 
GACAACTTCC AGTGTCCCCG GACCTCTGGA TTCCTGTGCA ATGACCGGGG TCGCTGTTCT 
ATGGGAGAGT GTGTGTGTGA GCCTGGTTGG ACAGGCCGCA GCTGTGACTG TCCCCTCAGC 
AATGCCACCT GCATCGATAG CAACGGGGGC ATCTGCAACG GCCGAGGCTA CTGTGAGTGT 
GGCCGTTGTC ACTGCAACCA GCAGTCGCTC TACACGGACA CCACCTGTGA GATCAACTAC 
TCTGCGATAC GCCTGGGTCT CTGTGAGGAT CTCCGCTCCT GCGTACAGTG CCAGGCCTGG 
GGCACCGGGG AGAAGAAAGG GCGCGCGTGT GACGATTGCC CCTTTAAAGT CAAGATGGTA 
GACGAGCTTA AGAAAGCAGA AGAGGTGGTG GAGTACTGCT CCTTCCGGGA TGAGGATGAC 
GACTGCACTT ACAGCTACAA CGTGGAGGGC GATGGCAGCC CTGGGCCCAA CAGCACAGTC 
CTGGTCCACA AAAAGAAAGA CTGCCCTCCC GGCTCCTTCT GGTGGCTCAT CCCCCTGCTC 
ATCTTCCTCC TGTTGCTCCT GGCGTTGCTT CTGCTGCTCT GCTGGAAATA CTGTGCCTGC 
TGCAAAGCCT GCCTGGGGCT TCTTCCTTGC TGCAACCGAG GTCACATGGT GGGCTTTAAG 
GAAGATCACT ATATGCTTCG GGAGAACCTG ATGGCCTCTG ACCACCTGGA CACGCCCATG 
CTACGAAGCG GGAACCTCAA GGGACGAGAC ACAGTCCGCT GGAAGATCAC CAACAATGTG 
CAGCGCCCTG GCTTTGCCAC CCATGCCGCC AGCACCAGCC CCACGGAGCT CGTACCCTAC 
GGGCTGTCCC TGCGCCTTGG CCGCCTCTGC ACTGAGAACC TTATGAAGCC GGGCACCCGA 
GAGTGTGACC AGCTACGCCA GGAGGTGGAG GAAAATCTGA ATGAGGTGTA TAGACAGGTC 
AGCGGCGCAC ACAAGCTCCA GCAGACGAAG TTCCGACAGC AGCCCAACAC CGGGAAAAAG 
CAAGACCACA CCATTGTGGA CACAGTGTTG CTGGCGCCCC GCTCCGCCAA GCAGATGCTG 
CTGAAGCTGA CAGAGAAGCA GGTGGAGCAG GGGTCCTTCC ATGAACTGAA GGTGGCCCCT 
GGCTACTACA CTGTCACGGC AGAGCAGGAT GCCCGGGGCA TGGTGGAGTT CCAGGAGGGC 
GTGGAGCTGG TGGATGTGCG AGTGCCCCTC TTCATCCGGC CTGAGGATGA TGATGAGAAG 
CAGCTGCTGG TGGAGGCCAT TGATGTCCCT GTGGGCACTG CCACCCTTGG TCGCCGTCTG 
GTAAACATCA CCATTATCAA GGAACAAGCT AGTGGGGTAG TGTCCTTCGA GCAGCCTGAA 
TACTCGGTGA GTCGTGGAGA CCAGGTGGCC CGCATCCCTG TCATCCGGCA CATCCTGGAC 
AATGGCAAGT CCCAGGTCTC CTATAGCACA CAGGATAATA CAGCACACGG ACACCGGGAT 
TATGTCCCTG TGGAGGGAGA GCTGCTGTTC CATCCTGGGG AGACCTGGAA GGAGTTGCAG 
GTGAAGCTAC TGGAGCTGCA GGAGGTCGAC TCCCTCCTGC GTGGCCGCCA GGTCCGCCGC 
TTCCAAGTCC AACTCAGCAA CCCCAAGTTC GGAGCCCGCC TGGGCCAGCC CAGCACAACC 
ACCGTTATTC TCGGTGAGCA CGATGAAACG GACAGGAGTC TCATAAATCA AACACTTTCA 
TCGCCTCCGC CACCCCATGG AGACCTGGGC GCCCCACAGA ACCCCAATGC CAAGGCTGCC 
GGGTCCAGGA AGATCCATTT CAACTGGCTG CCCCCTCCTG GCAAGCCAAT GGGGTACAGG 
GTGAAGTACT GGATCCAGGG CGACTCTGAG TCTGAAGCCC ACCTTCTAGA CAGCAAGGTG 
CCCTCAGTGG AACTCACCAA CCTGTATCCC TATTGCGACT ACGAAATGAA GGTGTGTGCC 
TATGGGGCAC AGGGTGAGGG GCCCTATAGC TCACTGGTGT CCTGCCGCAC CCACCAGGAA 
GTACCCAGCG AGCCAGGGAG GCTGGCTTTC AATGTAGTCT CTTCTACGGT GACGCAGCTG 
AGCTGGGCAG AGCCAGCTGA GACCAATGGC GAGATCACAG CCTACGAGGT CTGCTATGGA 
CTGGTCAATG AGGACAACCG ACCCATTGGA CCTATGAAGA AGGTGCTCGT GGACAACCCC 
AAGAACCGGA TGCTGCTCAT TGAGAATCTG CGAGAATCCC AGCCATACCG ATACACGGTT 
AAGGCGCGCA ATGGGGCAGG ATGGGGACCC GAGAGAGAGG CTATCATCAA CCTGGCTACA 
CAGCCCAAGC GGCCCATGTC CATCCCTATC ATCCCAGACA TCCCCATAGT GGACGCCCAG 
GGTGGAGAAG ACTACGAAAA CTTCCTTATG TACAGTGATG ACGTCCTGCG GTCCCCAGCC 
AGCAGCCAGA GGCCCAGCGT TTCTGATGAC ACTGAGCACC TGGTGAATGG CCGGATGGAC 
TTTGCCTATC CAGGCAGCGC CAACTCCCTG CACAGAATGA CTGCAGCCAA TGTGGCCTAT 
GGCACGCATC TGAGCCCACA CCTGTCCCAC CGAGTGCTGA GCACGTCCTC CACCCTTACC 
CGGGACTACC ACTCTCTGAC ACGCACAGAG CACTCCCACT CAGGCACACT TCCCAGGGAC 
TACTCCACCC TCACTTCCCT TTCCTCCCAA GGCCTCCCTC CTATCTGGGA AGATGGGAGG 
AGCAGGCTTC CGCTGTCCTG GACTCTTGGG TCCTTGAGCC GGGCTCACAT GAAGGGTGTG 
CCCGCATCCA GGGGTTCACC AGACTCTATA ATCCTGGCCG GGCAGTCAGC AGCACCCTCC 
TGGGGTACAG ATTCCCGTGG GGCTGTGGGT GTGCCTGACA CACCCACTCG GCTGGTGTTC 
TCTGCCCTGG GGCCCACGTC TTTGAAGGTG AGCTGGCAGG AGCCACAGTG TGATCGGATG 
CTGCTGGGCT ACAGTGTGGA ATACCAGCTA CTAAACGGCG GCGAGATGCA TCGGCTCAAC 
ATCCCTAACC CTGGCCAAAC CTCGGTGGTG GTAGAGGATC TCCTGCCTAA CCACTCCTAT 
GTGTTCCGGG TACGGGCCCA GAGCCAGGAG GGCTGGGGCC GAGAGCGAGA GGGTGTCATC 
ACCATCGAGT CCCAGGTGCA CCCGCAGAGC CCTCTCTGCC CCCTGCCAGG CTCAGCCTTC 
ACTCTGAGCA CCCCCAGCGC CCCGGGACCG CTGGTGTTCA CTGCCCTAAG CCCAGACTCC 
CTGCAGCTCA GCTGGGAGCG GCCGAGGAGG CCCAATGGAG ATATCCTTGG CTACCTGGTG 
ACCTGTGAGA TGGCCCAAGG AGGAGCACCA GCCAGGACCT TCCGGGTGGA CGGAGACAAC 
CCTGAGAGCC GGTTGACTGT ACCTGGCCTC AGTGAGAACG TTCCTTACAA GTTCAAGGTT 
CAGGCCAGGA CGACCGAGGG CTTCGGGCCA GAGCGTGAGG GTATCATCAC CATCGAGTCT 
CAGGTTGGAG GCCCCTTCCC ACAGCTGGGC AGCCATTCTG GGCTCTTCCA GAACCCAGTG 
CAAAGCGAGT TCAGCAGCGT GACCAGCACG CACAGCACCA CGACTGAGCC CTTCCTCATG 
GATGGTCTAA CCCTGGGGAC CCAGCGCCTG GAAGCAGGAG GCTCCCTCAC CCGGCATGTG 
ACCCAGGAAT TCGTGACCCG GACCTTAACG GCCAGTGGCT CTCTCAGCAC TCATATGGAC 
CAACAGTTCT TCCAAACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532636.1
CDS	436..5856
Translation	MAGPCCSPWVKLLLLAAMLSASLPGDLANRCKKAQVKSCTECIRVDKSCAYCTDELFKERRCNTQAELLAAGCRGESILVMESSLEITENTQIDTSLHRSQVSPQGLQVRLRPGEERSFVFQVFEPLESPVDLYILMDFSNSMSDDLDNLKQMGQNLAKILRQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTENVEEFWNKLQGERISGNLDAPEGGFDAILQTAVCTRDIGWRADSTHLLVFSTESAFHYEADGANVLAGIMNRNDEKCHLDASGAYTQYKTQDYPSVPTLVRLLAKHNIIPIFAVTNYSYSYYEKLHKYFPVSSLGVLQEDSSNIVELLEEAFYRIRSNLDIRALDSPRGLRTEVTSDTLQKTETGSFHIKRGEVGTYNVHLRAVEDIDGTHVCQLAKEDQGGNIHLKPSFSDGLRMDASVICDVCPCELQKEVRSARCHFRGDFMCGHCVCNEGWSGKTCNCSTGSLSDTQPCLREGEDKPCSGHGECQCGRCVCYGEGRYEGHFCEYDNFQCPRTSGFLCNDRGRCSMGECVCEPGWTGRSCDCPLSNATCIDSNGGICNGRGYCECGRCHCNQQSLYTDTTCEINYSAIRLGLCEDLRSCVQCQAWGTGEKKGRACDDCPFKVKMVDELKKAEEVVEYCSFRDEDDDCTYSYNVEGDGSPGPNSTVLVHKKKDCPPGSFWWLIPLLIFLLLLLALLLLLCWKYCACCKACLGLLPCCNRGHMVGFKEDHYMLRENLMASDHLDTPMLRSGNLKGRDTVRWKITNNVQRPGFATHAASTSPTELVPYGLSLRLGRLCTENLMKPGTRECDQLRQEVEENLNEVYRQVSGAHKLQQTKFRQQPNTGKKQDHTIVDTVLLAPRSAKQMLLKLTEKQVEQGSFHELKVAPGYYTVTAEQDARGMVEFQEGVELVDVRVPLFIRPEDDDEKQLLVEAIDVPVGTATLGRRLVNITIIKEQASGVVSFEQPEYSVSRGDQVARIPVIRHILDNGKSQVSYSTQDNTAHGHRDYVPVEGELLFHPGETWKELQVKLLELQEVDSLLRGRQVRRFQVQLSNPKFGARLGQPSTTTVILGEHDETDRSLINQTLSSPPPPHGDLGAPQNPNAKAAGSRKIHFNWLPPPGKPMGYRVKYWIQGDSESEAHLLDSKVPSVELTNLYPYCDYEMKVCAYGAQGEGPYSSLVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYGLVNEDNRPIGPMKKVLVDNPKNRMLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDIPIVDAQGGEDYENFLMYSDDVLRSPASSQRPSVSDDTEHLVNGRMDFAYPGSANSLHRMTAANVAYGTHLSPHLSHRVLSTSSTLTRDYHSLTRTEHSHSGTLPRDYSTLTSLSSQGLPPIWEDGRSRLPLSWTLGSLSRAHMKGVPASRGSPDSIILAGQSAAPSWGTDSRGAVGVPDTPTRLVFSALGPTSLKVSWQEPQCDRMLLGYSVEYQLLNGGEMHRLNIPNPGQTSVVVEDLLPNHSYVFRVRAQSQEGWGREREGVITIESQVHPQSPLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPNGDILGYLVTCEMAQGGAPARTFRVDGDNPESRLTVPGLSENVPYKFKVQARTTEGFGPEREGIITIESQVGGPFPQLGSHSGLFQNPVQSEFSSVTSTHSTTTEPFLMDGLTLGTQRLEAGGSLTRHVTQEFVTRTLTASGSLSTHMDQQFFQT

Target ORF information:

RefSeq Version	XM_006532573.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus integrin beta 4 (Itgb4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532573.3

ORF Insert Sequence:

ATGGCAGGGC CCTGTTGCAG CCCATGGGTG AAGCTGCTGC TGCTGGCAGC AATGCTGAGT 
GCCAGCCTCC CTGGAGACCT GGCCAACCGC TGCAAGAAGG CTCAGGTGAA GAGCTGTACC 
GAGTGCATCC GGGTGGACAA GAGCTGTGCC TACTGCACAG ACGAGCTGTT CAAGGAGAGG 
CGCTGCAACA CCCAGGCGGA GCTTCTGGCT GCAGGCTGCA GGGGAGAGAG CATCCTGGTC 
ATGGAGAGCA GCCTTGAAAT CACAGAGAAC ACCCAGATCG ACACCAGCCT GCACCGCAGC 
CAGGTATCTC CCCAAGGCCT GCAAGTCCGG CTGCGGCCGG GTGAGGAGCG CAGCTTTGTG 
TTCCAGGTGT TTGAGCCCCT GGAGAGCCCC GTGGATCTGT ATATCCTCAT GGACTTCTCC 
AACTCCATGT CTGACGATCT GGACAACCTC AAGCAGATGG GGCAGAACCT GGCCAAGATC 
CTGCGCCAGC TCACCAGCGA CTACACCATT GGATTTGGAA AGTTTGTGGA CAAAGTCAGC 
GTCCCACAGA CAGACATGAG GCCCGAGAAA CTGAAGGAGC CCTGGCCCAA CAGTGATCCC 
CCGTTCTCCT TCAAGAACGT TATCAGCTTA ACGGAGAATG TGGAAGAATT CTGGAACAAA 
CTGCAAGGAG AACGCATCTC AGGCAACCTG GACGCTCCTG AAGGGGGCTT TGATGCCATC 
CTGCAGACAG CTGTGTGCAC AAGGGACATT GGCTGGAGGG CTGACAGCAC CCACCTGCTG 
GTGTTCTCCA CCGAGTCTGC CTTCCACTAC GAGGCTGATG GTGCCAACGT GCTGGCCGGC 
ATCATGAACC GCAATGATGA GAAATGCCAC CTGGACGCCT CGGGCGCCTA CACCCAATAC 
AAGACACAGG ACTACCCATC AGTGCCCACG CTGGTTCGCC TGCTTGCCAA GCATAACATC 
ATCCCCATCT TTGCTGTCAC CAACTACTCT TACAGCTACT ATGAGAAGCT CCATAAGTAT 
TTCCCCGTCT CCTCTCTGGG CGTCCTGCAG GAGGATTCAT CCAACATCGT GGAGCTGCTG 
GAGGAGGCCT TCTATCGAAT TCGCTCCAAC CTGGACATCC GGGCTCTGGA CAGCCCCAGA 
GGCCTGAGAA CAGAGGTCAC CTCCGATACT CTCCAGAAGA CGGAGACTGG GTCCTTTCAC 
ATCAAGCGGG GGGAAGTGGG CACATACAAT GTGCATCTCC GGGCAGTGGA GGACATAGAT 
GGGACACATG TGTGCCAGCT GGCTAAAGAA GACCAAGGGG GCAACATCCA CCTGAAACCC 
TCCTTCTCTG ATGGCCTCCG GATGGACGCG AGTGTGATCT GTGACGTGTG CCCCTGTGAG 
CTGCAAAAGG AAGTTCGATC AGCTCGCTGT CACTTCAGAG GAGACTTCAT GTGTGGACAC 
TGTGTGTGCA ATGAGGGCTG GAGTGGCAAA ACCTGCAACT GCTCCACCGG CTCTCTGAGT 
GACACACAGC CCTGCCTGCG TGAGGGTGAG GACAAACCGT GCTCGGGCCA CGGCGAGTGC 
CAGTGCGGAC GCTGTGTGTG CTATGGTGAA GGCCGCTACG AGGGTCACTT CTGCGAGTAT 
GACAACTTCC AGTGTCCCCG GACCTCTGGA TTCCTGTGCA ATGACCGGGG TCGCTGTTCT 
ATGGGAGAGT GTGTGTGTGA GCCTGGTTGG ACAGGCCGCA GCTGTGACTG TCCCCTCAGC 
AATGCCACCT GCATCGATAG CAACGGGGGC ATCTGCAACG GCCGAGGCTA CTGTGAGTGT 
GGCCGTTGTC ACTGCAACCA GCAGTCGCTC TACACGGACA CCACCTGTGA GATCAACTAC 
TCTGCGATAC GCCTGGGTCT CTGTGAGGAT CTCCGCTCCT GCGTACAGTG CCAGGCCTGG 
GGCACCGGGG AGAAGAAAGG GCGCGCGTGT GACGATTGCC CCTTTAAAGT CAAGATGGTA 
GACGAGCTTA AGAAAGCAGA AGAGGTGGTG GAGTACTGCT CCTTCCGGGA TGAGGATGAC 
GACTGCACTT ACAGCTACAA CGTGGAGGGC GATGGCAGCC CTGGGCCCAA CAGCACAGTC 
CTGGTCCACA AAAAGAAAGA CTGCCCTCCC GGCTCCTTCT GGTGGCTCAT CCCCCTGCTC 
ATCTTCCTCC TGTTGCTCCT GGCGTTGCTT CTGCTGCTCT GCTGGAAATA CTGTGCCTGC 
TGCAAAGCCT GCCTGGGGCT TCTTCCTTGC TGCAACCGAG GTCACATGGT GGGCTTTAAG 
GAAGATCACT ATATGCTTCG GGAGAACCTG ATGGCCTCTG ACCACCTGGA CACGCCCATG 
CTACGAAGCG GGAACCTCAA GGGACGAGAC ACAGTCCGCT GGAAGATCAC CAACAATGTG 
CAGCGCCCTG GCTTTGCCAC CCATGCCGCC AGCACCAGCC CCACGGAGCT CGTACCCTAC 
GGGCTGTCCC TGCGCCTTGG CCGCCTCTGC ACTGAGAACC TTATGAAGCC GGGCACCCGA 
GAGTGTGACC AGCTACGCCA GGAGGTGGAG GAAAATCTGA ATGAGGTGTA TAGACAGGTC 
AGCGGCGCAC ACAAGCTCCA GCAGACGAAG TTCCGACAGC AGCCCAACAC CGGGAAAAAG 
CAAGACCACA CCATTGTGGA CACAGTGTTG CTGGCGCCCC GCTCCGCCAA GCAGATGCTG 
CTGAAGCTGA CAGAGAAGCA GGTGGAGCAG GGGTCCTTCC ATGAACTGAA GGTGGCCCCT 
GGCTACTACA CTGTCACGGC AGAGCAGGAT GCCCGGGGCA TGGTGGAGTT CCAGGAGGGC 
GTGGAGCTGG TGGATGTGCG AGTGCCCCTC TTCATCCGGC CTGAGGATGA TGATGAGAAG 
CAGCTGCTGG TGGAGGCCAT TGATGTCCCT GTGGGCACTG CCACCCTTGG TCGCCGTCTG 
GTAAACATCA CCATTATCAA GGAACAAGCT AGTGGGGTAG TGTCCTTCGA GCAGCCTGAA 
TACTCGGTGA GTCGTGGAGA CCAGGTGGCC CGCATCCCTG TCATCCGGCA CATCCTGGAC 
AATGGCAAGT CCCAGGTCTC CTATAGCACA CAGGATAATA CAGCACACGG ACACCGGGAT 
TATGTCCCTG TGGAGGGAGA GCTGCTGTTC CATCCTGGGG AGACCTGGAA GGAGTTGCAG 
GTGAAGCTAC TGGAGCTGCA GGAGGTCGAC TCCCTCCTGC GTGGCCGCCA GGTCCGCCGC 
TTCCAAGTCC AACTCAGCAA CCCCAAGTTC GGAGCCCGCC TGGGCCAGCC CAGCACAACC 
ACCGTTATTC TCGGTGAGCA CGATGAAACG GACAGGAGTC TCATAAATCA AACACTTTCA 
TCGCCTCCGC CACCCCATGG AGACCTGGGC GCCCCACAGA ACCCCAATGC CAAGGCTGCC 
GGGTCCAGGA AGATCCATTT CAACTGGCTG CCCCCTCCTG GCAAGCCAAT GGGGTACAGG 
GTGAAGTACT GGATCCAGGG CGACTCTGAG TCTGAAGCCC ACCTTCTAGA CAGCAAGGTG 
CCCTCAGTGG AACTCACCAA CCTGTATCCC TATTGCGACT ACGAAATGAA GGTGTGTGCC 
TATGGGGCAC AGGGTGAGGG GCCCTATAGC TCACTGGTGT CCTGCCGCAC CCACCAGGAA 
GTACCCAGCG AGCCAGGGAG GCTGGCTTTC AATGTAGTCT CTTCTACGGT GACGCAGCTG 
AGCTGGGCAG AGCCAGCTGA GACCAATGGC GAGATCACAG CCTACGAGGT CTGCTATGGA 
CTGGTCAATG AGGACAACCG ACCCATTGGA CCTATGAAGA AGGTGCTCGT GGACAACCCC 
AAGAACCGGA TGCTGCTCAT TGAGAATCTG CGAGAATCCC AGCCATACCG ATACACGGTT 
AAGGCGCGCA ATGGGGCAGG ATGGGGACCC GAGAGAGAGG CTATCATCAA CCTGGCTACA 
CAGCCCAAGC GGCCCATGTC CATCCCTATC ATCCCAGACA TCCCCATAGT GGACGCCCAG 
GGTGGAGAAG ACTACGAAAA CTTCCTTATG TACAGTGATG ACGTCCTGCG GTCCCCAGCC 
AGCAGCCAGA GGCCCAGCGT TTCTGATGAC ACTGAGCACC TGGTGAATGG CCGGATGGAC 
TTTGCCTATC CAGGCAGCGC CAACTCCCTG CACAGAATGA CTGCAGCCAA TGTGGCCTAT 
GGCACGCATC TGAGCCCACA CCTGTCCCAC CGAGTGCTGA GCACGTCCTC CACCCTTACC 
CGGGACTACC ACTCTCTGAC ACGCACAGAG CACTCCCACT CAGGCACACT TCCCAGGGAC 
TACTCCACCC TCACTTCCCT TTCCTCCCAA GGCCTCCCTC CTATCTGGGA AGATGGGAGG 
AGCAGGCTTC CGCTGTCCTG GACTCTTGGG TCCTTGAGCC GGGCTCACAT GAAGGGTGTG 
CCCGCATCCA GGGGTTCACC AGACTCTATA ATCCTGGCCG GGCAGTCAGC AGCACCCTCC 
TGGGGTACAG ATTCCCGTGG GGCTGTGGGT GTGCCTGACA CACCCACTCG GCTGGTGTTC 
TCTGCCCTGG GGCCCACGTC TTTGAAGGTG AGCTGGCAGG AGCCACAGTG TGATCGGATG 
CTGCTGGGCT ACAGTGTGGA ATACCAGCTA CTAAACGGCG GCGAGATGCA TCGGCTCAAC 
ATCCCTAACC CTGGCCAAAC CTCGGTGGTG GTAGAGGATC TCCTGCCTAA CCACTCCTAT 
GTGTTCCGGG TACGGGCCCA GAGCCAGGAG GGCTGGGGCC GAGAGCGAGA GGGTGTCATC 
ACCATCGAGT CCCAGGTGCA CCCGCAGAGC CCTCTCTGCC CCCTGCCAGG CTCAGCCTTC 
ACTCTGAGCA CCCCCAGCGC CCCGGGACCG CTGGTGTTCA CTGCCCTAAG CCCAGACTCC 
CTGCAGCTCA GCTGGGAGCG GCCGAGGAGG CCCAATGGAG ATATCCTTGG CTACCTGGTG 
ACCTGTGAGA TGGCCCAAGG AGGAGCACCA GCCAGGACCT TCCGGGTGGA CGGAGACAAC 
CCTGAGAGCC GGTTGACTGT ACCTGGCCTC AGTGAGAACG TTCCTTACAA GTTCAAGGTT 
CAGGCCAGGA CGACCGAGGG CTTCGGGCCA GAGCGTGAGG GTATCATCAC CATCGAGTCT 
CAGGTTGGAG GCCCCTTCCC ACAGCTGGGC AGCCATTCTG GGCTCTTCCA GAACCCAGTG 
CAAAGCGAGT TCAGCAGCGT GACCAGCACG CACAGCACCA CGACTGAGCC CTTCCTCATG 
GATGGTCTAA CCCTGGGGAC CCAGCGCCTG GAAGCAGGAG GCTCCCTCAC CCGGCATGTG 
ACCCAGGAAT TCGTGACCCG GACCTTAACG GCCAGTGGCT CTCTCAGCAC TCATATGGAC 
CAACAGTTCT TCCAAACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itgb4 ( XM_006532574.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532637.1 Mus musculus integrin beta 4 (Itgb4), transcript variant X6, mRNA.

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CloneID	OMu45810
Clone ID Related Accession (Same CDS sequence)	XM_006532574.2
Accession Version	XM_006532574.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	integrin beta-4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006532574.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGC CCTGTTGCAG CCCATGGGTG AAGCTGCTGC TGCTGGCAGC AATGCTGAGT 
GCCAGCCTCC CTGGAGACCT GGCCAACCGC TGCAAGAAGG CTCAGGTGAA GAGCTGTACC 
GAGTGCATCC GGGTGGACAA GAGCTGTGCC TACTGCACAG ACGAGCTGTT CAAGGAGAGG 
CGCTGCAACA CCCAGGCGGA GCTTCTGGCT GCAGGCTGCA GGGGAGAGAG CATCCTGGTC 
ATGGAGAGCA GCCTTGAAAT CACAGAGAAC ACCCAGATCG ACACCAGCCT GCACCGCAGC 
CAGGTATCTC CCCAAGGCCT GCAAGTCCGG CTGCGGCCGG GTGAGGAGCG CAGCTTTGTG 
TTCCAGGTGT TTGAGCCCCT GGAGAGCCCC GTGGATCTGT ATATCCTCAT GGACTTCTCC 
AACTCCATGT CTGACGATCT GGACAACCTC AAGCAGATGG GGCAGAACCT GGCCAAGATC 
CTGCGCCAGC TCACCAGCGA CTACACCATT GGATTTGGAA AGTTTGTGGA CAAAGTCAGC 
GTCCCACAGA CAGACATGAG GCCCGAGAAA CTGAAGGAGC CCTGGCCCAA CAGTGATCCC 
CCGTTCTCCT TCAAGAACGT TATCAGCTTA ACGGAGAATG TGGAAGAATT CTGGAACAAA 
CTGCAAGGAG AACGCATCTC AGGCAACCTG GACGCTCCTG AAGGGGGCTT TGATGCCATC 
CTGCAGACAG CTGTGTGCAC AAGGGACATT GGCTGGAGGG CTGACAGCAC CCACCTGCTG 
GTGTTCTCCA CCGAGTCTGC CTTCCACTAC GAGGCTGATG GTGCCAACGT GCTGGCCGGC 
ATCATGAACC GCAATGATGA GAAATGCCAC CTGGACGCCT CGGGCGCCTA CACCCAATAC 
AAGACACAGG ACTACCCATC AGTGCCCACG CTGGTTCGCC TGCTTGCCAA GCATAACATC 
ATCCCCATCT TTGCTGTCAC CAACTACTCT TACAGCTACT ATGAGAAGCT CCATAAGTAT 
TTCCCCGTCT CCTCTCTGGG CGTCCTGCAG GAGGATTCAT CCAACATCGT GGAGCTGCTG 
GAGGAGGCCT TCTATCGAAT TCGCTCCAAC CTGGACATCC GGGCTCTGGA CAGCCCCAGA 
GGCCTGAGAA CAGAGGTCAC CTCCGATACT CTCCAGAAGA CGGAGACTGG GTCCTTTCAC 
ATCAAGCGGG GGGAAGTGGG CACATACAAT GTGCATCTCC GGGCAGTGGA GGACATAGAT 
GGGACACATG TGTGCCAGCT GGCTAAAGAA GACCAAGGGG GCAACATCCA CCTGAAACCC 
TCCTTCTCTG ATGGCCTCCG GATGGACGCG AGTGTGATCT GTGACGTGTG CCCCTGTGAG 
CTGCAAAAGG AAGTTCGATC AGCTCGCTGT CACTTCAGAG GAGACTTCAT GTGTGGACAC 
TGTGTGTGCA ATGAGGGCTG GAGTGGCAAA ACCTGCAACT GCTCCACCGG CTCTCTGAGT 
GACACACAGC CCTGCCTGCG TGAGGGTGAG GACAAACCGT GCTCGGGCCA CGGCGAGTGC 
CAGTGCGGAC GCTGTGTGTG CTATGGTGAA GGCCGCTACG AGGGTCACTT CTGCGAGTAT 
GACAACTTCC AGTGTCCCCG GACCTCTGGA TTCCTGTGCA ATGACCGGGG TCGCTGTTCT 
ATGGGAGAGT GTGTGTGTGA GCCTGGTTGG ACAGGCCGCA GCTGTGACTG TCCCCTCAGC 
AATGCCACCT GCATCGATAG CAACGGGGGC ATCTGCAACG GCCGAGGCTA CTGTGAGTGT 
GGCCGTTGTC ACTGCAACCA GCAGTCGCTC TACACGGACA CCACCTGTGA GATCAACTAC 
TCTGCGATAC GCCTGGGTCT CTGTGAGGAT CTCCGCTCCT GCGTACAGTG CCAGGCCTGG 
GGCACCGGGG AGAAGAAAGG GCGCGCGTGT GACGATTGCC CCTTTAAAGT CAAGATGGTA 
GACGAGCTTA AGAAAGCAGA AGAGGTGGTG GAGTACTGCT CCTTCCGGGA TGAGGATGAC 
GACTGCACTT ACAGCTACAA CGTGGAGGGC GATGGCAGCC CTGGGCCCAA CAGCACAGTC 
CTGGTCCACA AAAAGAAAGA CTGCCCTCCC GGCTCCTTCT GGTGGCTCAT CCCCCTGCTC 
ATCTTCCTCC TGTTGCTCCT GGCGTTGCTT CTGCTGCTCT GCTGGAAATA CTGTGCCTGC 
TGCAAAGCCT GCCTGGGGCT TCTTCCTTGC TGCAACCGAG GTCACATGGT GGGCTTTAAG 
GAAGATCACT ATATGCTTCG GGAGAACCTG ATGGCCTCTG ACCACCTGGA CACGCCCATG 
CTACGAAGCG GGAACCTCAA GGGACGAGAC ACAGTCCGCT GGAAGATCAC CAACAATGTG 
CAGCGCCCTG GCTTTGCCAC CCATGCCGCC AGCACCAGCC CCACGGAGCT CGTACCCTAC 
GGGCTGTCCC TGCGCCTTGG CCGCCTCTGC ACTGAGAACC TTATGAAGCC GGGCACCCGA 
GAGTGTGACC AGCTACGCCA GGAGGTGGAG GAAAATCTGA ATGAGGTGTA TAGACAGGTC 
AGCGGCGCAC ACAAGCTCCA GCAGACGAAG TTCCGACAGC AGCCCAACAC CGGGAAAAAG 
CAAGACCACA CCATTGTGGA CACAGTGTTG CTGGCGCCCC GCTCCGCCAA GCAGATGCTG 
CTGAAGCTGA CAGAGAAGCA GGTGGAGCAG GGGTCCTTCC ATGAACTGAA GGTGGCCCCT 
GGCTACTACA CTGTCACGGC AGAGCAGGAT GCCCGGGGCA TGGTGGAGTT CCAGGAGGGC 
GTGGAGCTGG TGGATGTGCG AGTGCCCCTC TTCATCCGGC CTGAGGATGA TGATGAGAAG 
CAGCTGCTGG TGGAGGCCAT TGATGTCCCT GTGGGCACTG CCACCCTTGG TCGCCGTCTG 
GTAAACATCA CCATTATCAA GGAACAAGCT AGTGGGGTAG TGTCCTTCGA GCAGCCTGAA 
TACTCGGTGA GTCGTGGAGA CCAGGTGGCC CGCATCCCTG TCATCCGGCA CATCCTGGAC 
AATGGCAAGT CCCAGGTCTC CTATAGCACA CAGGATAATA CAGCACACGG ACACCGGGAT 
TATGTCCCTG TGGAGGGAGA GCTGCTGTTC CATCCTGGGG AGACCTGGAA GGAGTTGCAG 
GTGAAGCTAC TGGAGCTGCA GGAGGTCGAC TCCCTCCTGC GTGGCCGCCA GGTCCGCCGC 
TTCCAAGTCC AACTCAGCAA CCCCAAGTTC GGAGCCCGCC TGGGCCAGCC CAGCACAACC 
ACCGTTATTC TCGGTGAGCA CGATGAAACG GACAGGAGTC TCATAAATCA AACACTTTCA 
TCGCCTCCGC CACCCCATGG AGACCTGGGC GCCCCACAGA ACCCCAATGC CAAGGCTGCC 
GGGTCCAGGA AGATCCATTT CAACTGGCTG CCCCCTCCTG GCAAGCCAAT GGGGTACAGG 
GTGAAGTACT GGATCCAGGG CGACTCTGAG TCTGAAGCCC ACCTTCTAGA CAGCAAGGTG 
CCCTCAGTGG AACTCACCAA CCTGTATCCC TATTGCGACT ACGAAATGAA GGTGTGTGCC 
TATGGGGCAC AGGGTGAGGG GCCCTATAGC TCACTGGTGT CCTGCCGCAC CCACCAGGAA 
GTACCCAGCG AGCCAGGGAG GCTGGCTTTC AATGTAGTCT CTTCTACGGT GACGCAGCTG 
AGCTGGGCAG AGCCAGCTGA GACCAATGGC GAGATCACAG CCTACGAGGT CTGCTATGGA 
CTGGTCAATG AGGACAACCG ACCCATTGGA CCTATGAAGA AGGTGCTCGT GGACAACCCC 
AAGAACCGGA TGCTGCTCAT TGAGAATCTG CGAGAATCCC AGCCATACCG ATACACGGTT 
AAGGCGCGCA ATGGGGCAGG ATGGGGACCC GAGAGAGAGG CTATCATCAA CCTGGCTACA 
CAGCCCAAGC GGCCCATGTC CATCCCTATC ATCCCAGACA TCCCCATAGT GGACGCCCAG 
GGTGGAGAAG ACTACGAAAA CTTCCTTATG TACAGTGATG ACGTCCTGCG GTCCCCAGCC 
AGCAGCCAGA GGCCCAGCGT TTCTGATGAC ACTGAGCACC TGGTGAATGG CCGGATGGAC 
TTTGCCTATC CAGGCAGCGC CAACTCCCTG CACAGAATGA CTGCAGCCAA TGTGGCCTAT 
GGCACGCATC TGAGCCCACA CCTGTCCCAC CGAGTGCTGA GCACGTCCTC CACCCTTACC 
CGGGACTACC ACTCTCTGAC ACGCACAGAG CACTCCCACT CAGGCACACT TCCCAGGGAC 
TACTCCACCC TCACTTCCCT TTCCTCCCAA GATTCCCGTG GGGCTGTGGG TGTGCCTGAC 
ACACCCACTC GGCTGGTGTT CTCTGCCCTG GGGCCCACGT CTTTGAAGGT GAGCTGGCAG 
GAGCCACAGT GTGATCGGAT GCTGCTGGGC TACAGTGTGG AATACCAGCT ACTAAACGGC 
GGCGAGATGC ATCGGCTCAA CATCCCTAAC CCTGGCCAAA CCTCGGTGGT GGTAGAGGAT 
CTCCTGCCTA ACCACTCCTA TGTGTTCCGG GTACGGGCCC AGAGCCAGGA GGGCTGGGGC 
CGAGAGCGAG AGGGTGTCAT CACCATCGAG TCCCAGGTGC ACCCGCAGAG CCCTCTCTGC 
CCCCTGCCAG GCTCAGCCTT CACTCTGAGC ACCCCCAGCG CCCCGGGACC GCTGGTGTTC 
ACTGCCCTAA GCCCAGACTC CCTGCAGCTC AGCTGGGAGC GGCCGAGGAG GCCCAATGGA 
GATATCCTTG GCTACCTGGT GACCTGTGAG ATGGCCCAAG GAGGAGCACC AGCCAGGACC 
TTCCGGGTGG ACGGAGACAA CCCTGAGAGC CGGTTGACTG TACCTGGCCT CAGTGAGAAC 
GTTCCTTACA AGTTCAAGGT TCAGGCCAGG ACGACCGAGG GCTTCGGGCC AGAGCGTGAG 
GGTATCATCA CCATCGAGTC TCAGGTTGGA GGCCCCTTCC CACAGCTGGG CAGCCATTCT 
GGGCTCTTCC AGAACCCAGT GCAAAGCGAG TTCAGCAGCG TGACCAGCAC GCACAGCACC 
ACGACTGAGC CCTTCCTCAT GGATGGTCTA ACCCTGGGGA CCCAGCGCCT GGAAGCAGGA 
GGCTCCCTCA CCCGGCATGT GACCCAGGAA TTCGTGACCC GGACCTTAAC GGCCAGTGGC 
TCTCTCAGCA CTCATATGGA CCAACAGTTC TTCCAAACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532637.1
CDS	436..5697
Translation	MAGPCCSPWVKLLLLAAMLSASLPGDLANRCKKAQVKSCTECIRVDKSCAYCTDELFKERRCNTQAELLAAGCRGESILVMESSLEITENTQIDTSLHRSQVSPQGLQVRLRPGEERSFVFQVFEPLESPVDLYILMDFSNSMSDDLDNLKQMGQNLAKILRQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTENVEEFWNKLQGERISGNLDAPEGGFDAILQTAVCTRDIGWRADSTHLLVFSTESAFHYEADGANVLAGIMNRNDEKCHLDASGAYTQYKTQDYPSVPTLVRLLAKHNIIPIFAVTNYSYSYYEKLHKYFPVSSLGVLQEDSSNIVELLEEAFYRIRSNLDIRALDSPRGLRTEVTSDTLQKTETGSFHIKRGEVGTYNVHLRAVEDIDGTHVCQLAKEDQGGNIHLKPSFSDGLRMDASVICDVCPCELQKEVRSARCHFRGDFMCGHCVCNEGWSGKTCNCSTGSLSDTQPCLREGEDKPCSGHGECQCGRCVCYGEGRYEGHFCEYDNFQCPRTSGFLCNDRGRCSMGECVCEPGWTGRSCDCPLSNATCIDSNGGICNGRGYCECGRCHCNQQSLYTDTTCEINYSAIRLGLCEDLRSCVQCQAWGTGEKKGRACDDCPFKVKMVDELKKAEEVVEYCSFRDEDDDCTYSYNVEGDGSPGPNSTVLVHKKKDCPPGSFWWLIPLLIFLLLLLALLLLLCWKYCACCKACLGLLPCCNRGHMVGFKEDHYMLRENLMASDHLDTPMLRSGNLKGRDTVRWKITNNVQRPGFATHAASTSPTELVPYGLSLRLGRLCTENLMKPGTRECDQLRQEVEENLNEVYRQVSGAHKLQQTKFRQQPNTGKKQDHTIVDTVLLAPRSAKQMLLKLTEKQVEQGSFHELKVAPGYYTVTAEQDARGMVEFQEGVELVDVRVPLFIRPEDDDEKQLLVEAIDVPVGTATLGRRLVNITIIKEQASGVVSFEQPEYSVSRGDQVARIPVIRHILDNGKSQVSYSTQDNTAHGHRDYVPVEGELLFHPGETWKELQVKLLELQEVDSLLRGRQVRRFQVQLSNPKFGARLGQPSTTTVILGEHDETDRSLINQTLSSPPPPHGDLGAPQNPNAKAAGSRKIHFNWLPPPGKPMGYRVKYWIQGDSESEAHLLDSKVPSVELTNLYPYCDYEMKVCAYGAQGEGPYSSLVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYGLVNEDNRPIGPMKKVLVDNPKNRMLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDIPIVDAQGGEDYENFLMYSDDVLRSPASSQRPSVSDDTEHLVNGRMDFAYPGSANSLHRMTAANVAYGTHLSPHLSHRVLSTSSTLTRDYHSLTRTEHSHSGTLPRDYSTLTSLSSQDSRGAVGVPDTPTRLVFSALGPTSLKVSWQEPQCDRMLLGYSVEYQLLNGGEMHRLNIPNPGQTSVVVEDLLPNHSYVFRVRAQSQEGWGREREGVITIESQVHPQSPLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPNGDILGYLVTCEMAQGGAPARTFRVDGDNPESRLTVPGLSENVPYKFKVQARTTEGFGPEREGIITIESQVGGPFPQLGSHSGLFQNPVQSEFSSVTSTHSTTTEPFLMDGLTLGTQRLEAGGSLTRHVTQEFVTRTLTASGSLSTHMDQQFFQT

Target ORF information:

RefSeq Version	XM_006532574.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus integrin beta 4 (Itgb4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532574.2

ORF Insert Sequence:

ATGGCAGGGC CCTGTTGCAG CCCATGGGTG AAGCTGCTGC TGCTGGCAGC AATGCTGAGT 
GCCAGCCTCC CTGGAGACCT GGCCAACCGC TGCAAGAAGG CTCAGGTGAA GAGCTGTACC 
GAGTGCATCC GGGTGGACAA GAGCTGTGCC TACTGCACAG ACGAGCTGTT CAAGGAGAGG 
CGCTGCAACA CCCAGGCGGA GCTTCTGGCT GCAGGCTGCA GGGGAGAGAG CATCCTGGTC 
ATGGAGAGCA GCCTTGAAAT CACAGAGAAC ACCCAGATCG ACACCAGCCT GCACCGCAGC 
CAGGTATCTC CCCAAGGCCT GCAAGTCCGG CTGCGGCCGG GTGAGGAGCG CAGCTTTGTG 
TTCCAGGTGT TTGAGCCCCT GGAGAGCCCC GTGGATCTGT ATATCCTCAT GGACTTCTCC 
AACTCCATGT CTGACGATCT GGACAACCTC AAGCAGATGG GGCAGAACCT GGCCAAGATC 
CTGCGCCAGC TCACCAGCGA CTACACCATT GGATTTGGAA AGTTTGTGGA CAAAGTCAGC 
GTCCCACAGA CAGACATGAG GCCCGAGAAA CTGAAGGAGC CCTGGCCCAA CAGTGATCCC 
CCGTTCTCCT TCAAGAACGT TATCAGCTTA ACGGAGAATG TGGAAGAATT CTGGAACAAA 
CTGCAAGGAG AACGCATCTC AGGCAACCTG GACGCTCCTG AAGGGGGCTT TGATGCCATC 
CTGCAGACAG CTGTGTGCAC AAGGGACATT GGCTGGAGGG CTGACAGCAC CCACCTGCTG 
GTGTTCTCCA CCGAGTCTGC CTTCCACTAC GAGGCTGATG GTGCCAACGT GCTGGCCGGC 
ATCATGAACC GCAATGATGA GAAATGCCAC CTGGACGCCT CGGGCGCCTA CACCCAATAC 
AAGACACAGG ACTACCCATC AGTGCCCACG CTGGTTCGCC TGCTTGCCAA GCATAACATC 
ATCCCCATCT TTGCTGTCAC CAACTACTCT TACAGCTACT ATGAGAAGCT CCATAAGTAT 
TTCCCCGTCT CCTCTCTGGG CGTCCTGCAG GAGGATTCAT CCAACATCGT GGAGCTGCTG 
GAGGAGGCCT TCTATCGAAT TCGCTCCAAC CTGGACATCC GGGCTCTGGA CAGCCCCAGA 
GGCCTGAGAA CAGAGGTCAC CTCCGATACT CTCCAGAAGA CGGAGACTGG GTCCTTTCAC 
ATCAAGCGGG GGGAAGTGGG CACATACAAT GTGCATCTCC GGGCAGTGGA GGACATAGAT 
GGGACACATG TGTGCCAGCT GGCTAAAGAA GACCAAGGGG GCAACATCCA CCTGAAACCC 
TCCTTCTCTG ATGGCCTCCG GATGGACGCG AGTGTGATCT GTGACGTGTG CCCCTGTGAG 
CTGCAAAAGG AAGTTCGATC AGCTCGCTGT CACTTCAGAG GAGACTTCAT GTGTGGACAC 
TGTGTGTGCA ATGAGGGCTG GAGTGGCAAA ACCTGCAACT GCTCCACCGG CTCTCTGAGT 
GACACACAGC CCTGCCTGCG TGAGGGTGAG GACAAACCGT GCTCGGGCCA CGGCGAGTGC 
CAGTGCGGAC GCTGTGTGTG CTATGGTGAA GGCCGCTACG AGGGTCACTT CTGCGAGTAT 
GACAACTTCC AGTGTCCCCG GACCTCTGGA TTCCTGTGCA ATGACCGGGG TCGCTGTTCT 
ATGGGAGAGT GTGTGTGTGA GCCTGGTTGG ACAGGCCGCA GCTGTGACTG TCCCCTCAGC 
AATGCCACCT GCATCGATAG CAACGGGGGC ATCTGCAACG GCCGAGGCTA CTGTGAGTGT 
GGCCGTTGTC ACTGCAACCA GCAGTCGCTC TACACGGACA CCACCTGTGA GATCAACTAC 
TCTGCGATAC GCCTGGGTCT CTGTGAGGAT CTCCGCTCCT GCGTACAGTG CCAGGCCTGG 
GGCACCGGGG AGAAGAAAGG GCGCGCGTGT GACGATTGCC CCTTTAAAGT CAAGATGGTA 
GACGAGCTTA AGAAAGCAGA AGAGGTGGTG GAGTACTGCT CCTTCCGGGA TGAGGATGAC 
GACTGCACTT ACAGCTACAA CGTGGAGGGC GATGGCAGCC CTGGGCCCAA CAGCACAGTC 
CTGGTCCACA AAAAGAAAGA CTGCCCTCCC GGCTCCTTCT GGTGGCTCAT CCCCCTGCTC 
ATCTTCCTCC TGTTGCTCCT GGCGTTGCTT CTGCTGCTCT GCTGGAAATA CTGTGCCTGC 
TGCAAAGCCT GCCTGGGGCT TCTTCCTTGC TGCAACCGAG GTCACATGGT GGGCTTTAAG 
GAAGATCACT ATATGCTTCG GGAGAACCTG ATGGCCTCTG ACCACCTGGA CACGCCCATG 
CTACGAAGCG GGAACCTCAA GGGACGAGAC ACAGTCCGCT GGAAGATCAC CAACAATGTG 
CAGCGCCCTG GCTTTGCCAC CCATGCCGCC AGCACCAGCC CCACGGAGCT CGTACCCTAC 
GGGCTGTCCC TGCGCCTTGG CCGCCTCTGC ACTGAGAACC TTATGAAGCC GGGCACCCGA 
GAGTGTGACC AGCTACGCCA GGAGGTGGAG GAAAATCTGA ATGAGGTGTA TAGACAGGTC 
AGCGGCGCAC ACAAGCTCCA GCAGACGAAG TTCCGACAGC AGCCCAACAC CGGGAAAAAG 
CAAGACCACA CCATTGTGGA CACAGTGTTG CTGGCGCCCC GCTCCGCCAA GCAGATGCTG 
CTGAAGCTGA CAGAGAAGCA GGTGGAGCAG GGGTCCTTCC ATGAACTGAA GGTGGCCCCT 
GGCTACTACA CTGTCACGGC AGAGCAGGAT GCCCGGGGCA TGGTGGAGTT CCAGGAGGGC 
GTGGAGCTGG TGGATGTGCG AGTGCCCCTC TTCATCCGGC CTGAGGATGA TGATGAGAAG 
CAGCTGCTGG TGGAGGCCAT TGATGTCCCT GTGGGCACTG CCACCCTTGG TCGCCGTCTG 
GTAAACATCA CCATTATCAA GGAACAAGCT AGTGGGGTAG TGTCCTTCGA GCAGCCTGAA 
TACTCGGTGA GTCGTGGAGA CCAGGTGGCC CGCATCCCTG TCATCCGGCA CATCCTGGAC 
AATGGCAAGT CCCAGGTCTC CTATAGCACA CAGGATAATA CAGCACACGG ACACCGGGAT 
TATGTCCCTG TGGAGGGAGA GCTGCTGTTC CATCCTGGGG AGACCTGGAA GGAGTTGCAG 
GTGAAGCTAC TGGAGCTGCA GGAGGTCGAC TCCCTCCTGC GTGGCCGCCA GGTCCGCCGC 
TTCCAAGTCC AACTCAGCAA CCCCAAGTTC GGAGCCCGCC TGGGCCAGCC CAGCACAACC 
ACCGTTATTC TCGGTGAGCA CGATGAAACG GACAGGAGTC TCATAAATCA AACACTTTCA 
TCGCCTCCGC CACCCCATGG AGACCTGGGC GCCCCACAGA ACCCCAATGC CAAGGCTGCC 
GGGTCCAGGA AGATCCATTT CAACTGGCTG CCCCCTCCTG GCAAGCCAAT GGGGTACAGG 
GTGAAGTACT GGATCCAGGG CGACTCTGAG TCTGAAGCCC ACCTTCTAGA CAGCAAGGTG 
CCCTCAGTGG AACTCACCAA CCTGTATCCC TATTGCGACT ACGAAATGAA GGTGTGTGCC 
TATGGGGCAC AGGGTGAGGG GCCCTATAGC TCACTGGTGT CCTGCCGCAC CCACCAGGAA 
GTACCCAGCG AGCCAGGGAG GCTGGCTTTC AATGTAGTCT CTTCTACGGT GACGCAGCTG 
AGCTGGGCAG AGCCAGCTGA GACCAATGGC GAGATCACAG CCTACGAGGT CTGCTATGGA 
CTGGTCAATG AGGACAACCG ACCCATTGGA CCTATGAAGA AGGTGCTCGT GGACAACCCC 
AAGAACCGGA TGCTGCTCAT TGAGAATCTG CGAGAATCCC AGCCATACCG ATACACGGTT 
AAGGCGCGCA ATGGGGCAGG ATGGGGACCC GAGAGAGAGG CTATCATCAA CCTGGCTACA 
CAGCCCAAGC GGCCCATGTC CATCCCTATC ATCCCAGACA TCCCCATAGT GGACGCCCAG 
GGTGGAGAAG ACTACGAAAA CTTCCTTATG TACAGTGATG ACGTCCTGCG GTCCCCAGCC 
AGCAGCCAGA GGCCCAGCGT TTCTGATGAC ACTGAGCACC TGGTGAATGG CCGGATGGAC 
TTTGCCTATC CAGGCAGCGC CAACTCCCTG CACAGAATGA CTGCAGCCAA TGTGGCCTAT 
GGCACGCATC TGAGCCCACA CCTGTCCCAC CGAGTGCTGA GCACGTCCTC CACCCTTACC 
CGGGACTACC ACTCTCTGAC ACGCACAGAG CACTCCCACT CAGGCACACT TCCCAGGGAC 
TACTCCACCC TCACTTCCCT TTCCTCCCAA GATTCCCGTG GGGCTGTGGG TGTGCCTGAC 
ACACCCACTC GGCTGGTGTT CTCTGCCCTG GGGCCCACGT CTTTGAAGGT GAGCTGGCAG 
GAGCCACAGT GTGATCGGAT GCTGCTGGGC TACAGTGTGG AATACCAGCT ACTAAACGGC 
GGCGAGATGC ATCGGCTCAA CATCCCTAAC CCTGGCCAAA CCTCGGTGGT GGTAGAGGAT 
CTCCTGCCTA ACCACTCCTA TGTGTTCCGG GTACGGGCCC AGAGCCAGGA GGGCTGGGGC 
CGAGAGCGAG AGGGTGTCAT CACCATCGAG TCCCAGGTGC ACCCGCAGAG CCCTCTCTGC 
CCCCTGCCAG GCTCAGCCTT CACTCTGAGC ACCCCCAGCG CCCCGGGACC GCTGGTGTTC 
ACTGCCCTAA GCCCAGACTC CCTGCAGCTC AGCTGGGAGC GGCCGAGGAG GCCCAATGGA 
GATATCCTTG GCTACCTGGT GACCTGTGAG ATGGCCCAAG GAGGAGCACC AGCCAGGACC 
TTCCGGGTGG ACGGAGACAA CCCTGAGAGC CGGTTGACTG TACCTGGCCT CAGTGAGAAC 
GTTCCTTACA AGTTCAAGGT TCAGGCCAGG ACGACCGAGG GCTTCGGGCC AGAGCGTGAG 
GGTATCATCA CCATCGAGTC TCAGGTTGGA GGCCCCTTCC CACAGCTGGG CAGCCATTCT 
GGGCTCTTCC AGAACCCAGT GCAAAGCGAG TTCAGCAGCG TGACCAGCAC GCACAGCACC 
ACGACTGAGC CCTTCCTCAT GGATGGTCTA ACCCTGGGGA CCCAGCGCCT GGAAGCAGGA 
GGCTCCCTCA CCCGGCATGT GACCCAGGAA TTCGTGACCC GGACCTTAAC GGCCAGTGGC 
TCTCTCAGCA CTCATATGGA CCAACAGTTC TTCCAAACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Itgb4
Entrez Gene ID	192897
Full Name	integrin beta 4
Synonyms	AA407042,C230078O20,CD104
General protein information	Preferred Names integrin beta 4 Names integrin beta-4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 E2\|11 80.91 cM
Summary	Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008].

Bos taurus (cattle)	ITGB4	NP_001180186.2
Danio rerio (zebrafish)	itgb4	NP_001019557.1
Pan troglodytes (chimpanzee)	ITGB4	XP_003315785.1
Rattus norvegicus (Norway rat)	Itgb4	NP_037312.1
Xenopus tropicalis (tropical clawed frog)	itgb4	XP_004918514.1
Canis lupus familiaris (dog)	ITGB4	XP_005624218.1
Gallus gallus (chicken)	ITGB4	XP_003642374.2
Homo sapiens (human)	ITGB4	NP_000204.3
	ITGB4	XP_002800666.1
Mus musculus (house mouse)	Itgb4	NP_001005608.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Itgb4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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