ITGB4 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ITGB4
Entrez Gene ID	506995
Full Name	integrin subunit beta 4
General protein information	Preferred Names integrin subunit beta 4 Names integrin beta-4 integrin, beta 4
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024979795.1	XP_024835563.1	integrin beta-4 isoform X1
XM_024979796.1	XP_024835564.1	integrin beta-4 isoform X2
XM_024979798.1	XP_024835566.1	integrin beta-4 isoform X4
NM_001193257.2	NP_001180186.2	integrin beta-4 precursor
XM_024979797.1	XP_024835565.1	integrin beta-4 isoform X3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ITGB4	NP_001180186.2
Danio rerio (zebrafish)	itgb4	NP_001019557.1
Pan troglodytes (chimpanzee)	ITGB4	XP_003315785.1
Rattus norvegicus (Norway rat)	Itgb4	NP_037312.1
Xenopus tropicalis (tropical clawed frog)	itgb4	XP_004918514.1
Canis lupus familiaris (dog)	ITGB4	XP_005624218.1
Gallus gallus (chicken)	ITGB4	XP_003642374.2
Homo sapiens (human)	ITGB4	NP_000204.3
	ITGB4	XP_002800666.1
Mus musculus (house mouse)	Itgb4	NP_001005608.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

integrin alpha6beta4 was indeed involved in dairy cow mammary development.
Title: Up-regulation of integrin ?6?4 expression by mitogens involved in dairy cow mammary development.

The following ITGB4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ITGB4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa226109	XM_024979795.1 Latest version!	Bos taurus integrin subunit beta 4 (ITGB4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa226110	XM_024979796.1 Latest version!	Bos taurus integrin subunit beta 4 (ITGB4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa226111	XM_024979797.1 Latest version!	Bos taurus integrin subunit beta 4 (ITGB4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa226112	XM_024979798.1 Latest version!	Bos taurus integrin subunit beta 4 (ITGB4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa01779	NM_001193257.2 Latest version!	Bos taurus integrin, beta 4 (ITGB4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ITGB4 ( XM_024979795.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835563.1 Bos taurus integrin subunit beta 4 (ITGB4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa226109
Clone ID Related Accession (Same CDS sequence)	XM_024979795.1
Accession Version	XM_024979795.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	integrin beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGC CACATCCCAG CCCGTGGACC AGGCTGCTGC TGGCAGCGCT GCTGAGCGTC 
AGCTTCCCCG GGCACATGGC GAACCGCTGC AAGAAGGCCC AGGTGAAGAG CTGTACCGAG 
TGCATCCGCG TGGACAAGGA CTGCGCCTAC TGCACGGACG AGGTGTTCAA GGAACGGCGC 
TGTAACACCC AGGCCGAGCT GCTGGCTGCC GGCTGCCGGC TGGAGAGCGT GGTGGTCATG 
GAGAGCAGCT TCGAGATCAC GGAGGAAAGG CAGATCGACA CCACGCTGCG CCGCAGCCAG 
GTGTCCCCCC AGGCGCTGCG GGTGCGGCTG CGGCCGGGCG AGGAGCGGCA CTTCGAGCTG 
CAGGTGTTCG AGCCCCTGGA GAGCCCCATG GACCTGTATA TCCTCATGGA CTTCTCCAAC 
TCCATGTCTG ATGATCTGGA CAACCTCAAG AAGATGGGGC AAGATCTGGC TCAGGTCCTG 
AGGCAGCTCA CCAGTGACTA CACAATAGGA TTTGGCAAGT TCGTGGACAA AGTCAGCGTC 
CCTCAGACAG ACATGAGGCC TGAGAAGCTG AAGGAACCCT GGCCCAACAG CGACCCCCCC 
TTCTCCTTCA AGAATGTCAT CAGCCTGACG GAAGATGTGG AGGAGTTCCG GAATAAGCTG 
AAGGGGGAGC GCATCTCAGG CAACCTGGAT GCCCCGGAAG GAGGTTTTGA TGCCATCCTG 
CAGACGGCCG TGTGCACCAG TGACATTGGC TGGCGCCCTG ACAGCACCCA CTTGCTGGTG 
TTCTCCACCG AGTCAGCCTT CCACTACGAG GCCGACGGGG CCAACGTGCT GGCCGGCATC 
ATGAGGCGTA ACGACGAGGA ATGCCACCTG GACCCCACGG GCACCTACAC CCAGTACAAG 
ATGCAGGACT ACCCGTCGGT GCCCACCCTG GTGCGCCTGC TCGGCCAACA CAACATCATC 
CCCATCTTCG CCGTCACCAA CTACTCCTAT AGCTACTATG AGAGGCTGTC GTCATATTTC 
CCCGTCTCTT CACTGGGGGT GCTGCAGGAG GACTCGTCCA ACATTGTGGA TCTGCTGCAG 
GAAGCCTTCA ACCGCATCCG CTCCAACCTG GACATTCGGG CCCTGGACAG TCCTCGAGGC 
CTGCGGACAG AGGTCACCTC TAGGATGTTC CAGAAGACGG ACACGGGGTC CTTTTTCATC 
CGGCGAGGGG AAGTGGGCAC ATACCAAGTG CAGCTCCGGG CCATCGAGGA CCTGGACGGG 
GCTCATGTGT GTCAGCTTCT GGAGGCAGAC CAGAAGGGCA CCATCCATCT GAAGCCATCC 
TTCTCCAACG GCCTCCGGAT GGACGTGGGC GTCATCTGTG ACGTGTGCTC CTGTGAGCTG 
CAAAAAGAGG AGCGGTCATC TCGCTGCAAC TTCCACGGGG ACTTCATGTG CGGACACTGT 
GTGTGCCACG AGGGCTGGAG CGGCAAGACC TGTAACTGCT CCACCGGCTC CCAGAGCGAC 
CTGCAGCCCT GCCGGCGGGA GGGCGAGGAC AAGGTGTGCT CCGGGCGGGG CGAGTGCCAG 
TGTGGCCACT GTGTGTGCTA TGGCGAAGGC CGCTACGAGG GTCAGTTCTG CGAATACGAC 
AACTTCCAGT GTCCCCGCAC CTCCGGGTTC CTCTGCAATG ACCGGGGACG CTGCTCCATG 
GGTCAGTGCG TGTGTGAGCC TGGCTGGACA GGCCTGAGCT GTGACTGTCC TCTCAGCAAT 
GCCACCTGCA TCGACAGTAA CGGGGGTCTC TGTAATGGGC GTGGCCACTG CGAGTGCGGC 
CGCTGCCACT GCAACCAACA GTCGCTCTAC ACGGACACCG TCTGCGAGAT CAACTACTCG 
GCGATCCACC TGGGCCTCTG TGAGGACCTG CGCTCCTGCG TGCAGTGCCA GGCCTGGGGC 
ACCGGTGAAA AGAAGGGGCG CACGTGCGAG GAGTGCAGCT TCAAGGTCAA GATGGTGGAT 
GAGCTCAAGA AAGCGGAGGA GGTGGTGGAG CACTGCTCCT TCCGGGACGA GGACGACGAC 
TGTACCTACA GTTACACCGT GGAGGGGGAC AGCGCCCCCG GGCCCAACAG CACCGTCTTG 
GTGCAGAGGA GGAAGGAATG CCCCCCGGGC ACCTTCTGGT GGCTCATCCC CCTGCTCATC 
TTCCTCCTCC TGTTCCCGGT CCTGCTGCTG CTGCTCTGCT GGAAGTACTG TGCCTGCTGC 
AAGGCCTGCC TGGCCCTTCT CCCTTGCTGC AACCGAGGTC ACATGGTGGG CTTCAAGGAA 
GACCATTACA TGCTGCGAGA GAACCTGATG GCCTCGGACC ACTTGGACAC GCCCTTGCTG 
CGCAGCGGGA ACCTCAAGGG TCGCGACACA GTCCGATGGA AGATCACCAA CAACGTGCAG 
CGGCCGGGCT TCGCCTCGCA CGCTGCAGGC ATCAACCCCT CAGAGCTGGT GCCCTACGGA 
CTGTCCCTGC GGCTTGCTCG CCTCTGCACC GAGAACCTGC TGAAGCCTGG CACGCGAGAA 
TGCGACCAGC TGCGCCAGGA GGTGGAGGAG AATCTGAACG AGGTGTACCG ACAAATCACA 
GGCGCTCACA ACCTCCAGCA GACCAAGTTC CGGCAGCAGC CCAACGCCGG GAAAAAGCAG 
GACCACACCA TTGTGGACAC TGTGCTGATG GCTCCCCGCT CGGCCAAGCA GGCCCTGCTG 
AAGCTGACGG AGAAGCACGT GGAGCAGGGG GCCTTCCATG AGCTCAAGGT GGCCCCCGGC 
TATTACACGC TCACCGCAGA CCAGGATGCC CGGGGCATGG TGGAATTCCA GGAGGGCGTG 
GAGCTGGTGG ACGTGCGGGT GCCTTTGTTT ATCCGGCCCG AGGACGACGA CGAGAAGCAG 
CTGCTGGTGG AGGCCATCGA CGTGCCTGTG GGCACCGCCA CCCTCGGACG TCGCCTGGTC 
AATATCACCA TCATCAAGGA GCAAGCCAGC GGGATCGTGT CCTTTGAGCA GCCCGAGTAC 
TTGGTCAGCA GCGGGGAGCA CGTTGCCCGC ATCCCCGTGG TCCGGCGCAT CCTGGACAGC 
GGCAAATCAC AGGTCTCCTA CCGCACACAG GATAACACCG CCAAGGCCAA CCGGGACTAC 
ATCCCCATGG AGGGTGAGCT GCTGTTCCAA CCTGGGGAGA CCTGGAAGGA GCTGCAGGTG 
AAGCTCCTGG AGCTGCAGGA GATGGACTCC CTTCTGCGGG GCCCCCAGAC CCGCCGCTTC 
TACATCCAAC TCAGCAACCC CAAGTTCGGG GCCCGCCTGG GCCAGCCCCA GTGTGCCACT 
GTCATCACTG GGGACCGAGA CGAGCTGGAC CGGAATGTAA TGAACCAGAC CGTGTCATCA 
CCACCACCGC CCCGGGGTGA CCTGGGTGCC CCACAGAACC CCAATGCCAA GGCTGCTGGG 
TCCCGGAAGA TCCACTTCAA CTGGCTGCCT CCTCCTGGCA AGCCGACAGG GTACCGGGTG 
AAATATTGGA TCCAGGGTGA CTCCGAGTCT GAAGCCCACC TGCTTGACAG CAAGGTGCCC 
TCAGTGGAGC TCACCAACCT GTACCCATAT TGTGACTACG AGATGAAGGT GTGCGCCTAT 
GGGGCGCAGG GCGAGGGCCC CTACAGCTCC CCGGTGTCCT GTCGCACCCA CCAGGAAGTG 
CCCAGCGAGC CCGGGCGTCT GGCCTTCAAC GTCGTCTCCT CCACCGTGAC CCAGCTGAGC 
TGGGCCGAGC CGGCTGAGAC CAACGGCGAG ATCACAGCCT ACGAGGTCTG CTATGGCCTG 
GTCAATGAGG ACAACCGACC CATCGGGCCC ATGAAGAAGG TGCTGGTGGA CAGCCCCAAG 
AAGCGGACGC TGCTCATCGA GAACCTGCGC GAGTCCCAGC CATACCGCTA CACGGTCAAG 
GCTCGCAACG GGGCAGGCTG GGGGCCTGAG CGGGAGGCCA TCATCAACCT GGCCACCCAG 
CCCAAGCGAC CCATGTCCAT CCCCATCATC CCTGACATCC CCATCGTGGA CGCCCAGAGT 
GGGGAGGACT ATGAGAGCTT CCTCATGTAC AGCGATGATG TGCTCCGCTC TCCAGCCGGC 
AGCCAGAGGC CCAGCGTCTC TGATGACACC GGCTGCGGCT GGAAGTTCGA GCCCCTGCTG 
GGGGAGGAGC TGGACCTGAG GCGCGTCACG TGGCGGCTGC CCCCGGAGCT CATCCCGCGC 
CTGTCCGCCG GCAGCCGGCG CTCCTCCGAC TCCGGGCCCC CGGGAGACGG CGCCGCCGAT 
GGGGCCGATG GGGGCACGCG GGGCGCCAGC GGGGAGGGAG AGCACCTGGT GAACGGGCGG 
ATGGACTTTG CCTTCCCGGG CAGCGCCAAC TCCCTGCACA GGATGACCGT GAGCACCGCG 
GCCCACGGCG CCCACCTGAG CCCGCAGTTG TCCCACCGCG TGCTGAGCAC CTCGTCCACC 
CTCACGCGGG ACTACCACTC GCTGACCCGC ACGGAGCACT CGCACTCCGC CACGCTGCCC 
AGGGACTACT CCACTCTCAC CTCCCTTTCC TCCCAAAGCC TCCCTCCCAT CTGGGAACAC 
GGGAGGAGCA GGCTTCCGCT GTCCTGGGCC CTAGGGTCCC GGAGTCGGGC TCAGATGAAG 
GGAGCCCCCC GCTCCGGGGA CTTGAGAGAC TCTATAATCC TGGCTGGGCA GCCAGCAGCG 
CCCTCTTGGG GCCCAGGCTC CCGCTTGGCT GCGGGTGTGC CCAACACACC CACCCGCCTG 
GTGTTCTCCG CCCTTGGACC CACGTCTCTG AAAGTGAGCT GGCAGGAGCC GCAGTGTGAG 
CGTGCGCTGC AGGGCTACAG TGTGGAGTAC CAGCTGCTGA ACGGCGGTGA GCTGTATCGC 
CTCAACATCC CCAACCCCAG CCAGACCTCG GTGGTGGTGG AGGACCTCCT GCCCAACCAC 
TCCTACGTGT TCCGTGTGCG GGCCCAGAGC CAGGAGGGCT GGGGCCCGGA GCGTGAGGGT 
GTCATCACCA TTGAGTCACA GGTGCACCCG CAGAGCCCGC TCTGCCCCCT GCCGGGCTCC 
GCCTTCACTT TGAGCACACC CAGCGCCCCG GGCCCGCTGG TGTTCACTGC CCTGAGCCCC 
GACTCGCTGC AGCTGAGCTG GGAGCGGCCC CGCAGGCCAG ATGGCGACAT TCTGGGCTAC 
CTGGTGACGT GTGAGATGGC CCACGGAGGA GAGCCAGCCA CTACGTTCCT GGTTGACGGC 
GACAGCCCTG AGAGCCGGCT GACCGTGCCT GGCCTCAGCG AGAACGTGCC CTACAAGTTC 
AAGGTGCAGG CCAAGACCAC CCAAGGCTTC GGGCCGGAGC GAGAGGGAAT CATCACCATC 
GAGTCCCAGG ATGGAGGACC CTTTCCACAG CTGGGCGGCC ACTTGGGGCT CTTCCAGCAC 
CCAGCGTCAG GGGAATACAG CAGCATCACC ACCCACTCCA GCACCACTGA ACCCTTCCTA 
CTGGATGGAC TGACCCTGGG CTCCCAGCAT CTGGAAGCAA GTGGCTCCCT CACCCGGCAT 
GTGACACAGG AGTTTGTGAG CCGGACTCTG ACCACCAGTG GGACCCTCAG CACCCACGTG 
GACCAACAGT TCTTCCAGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835563.1
CDS	231..5834
Translation	MAGPHPSPWTRLLLAALLSVSFPGHMANRCKKAQVKSCTECIRVDKDCAYCTDEVFKERRCNTQAELLAAGCRLESVVVMESSFEITEERQIDTTLRRSQVSPQALRVRLRPGEERHFELQVFEPLESPMDLYILMDFSNSMSDDLDNLKKMGQDLAQVLRQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTEDVEEFRNKLKGERISGNLDAPEGGFDAILQTAVCTSDIGWRPDSTHLLVFSTESAFHYEADGANVLAGIMRRNDEECHLDPTGTYTQYKMQDYPSVPTLVRLLGQHNIIPIFAVTNYSYSYYERLSSYFPVSSLGVLQEDSSNIVDLLQEAFNRIRSNLDIRALDSPRGLRTEVTSRMFQKTDTGSFFIRRGEVGTYQVQLRAIEDLDGAHVCQLLEADQKGTIHLKPSFSNGLRMDVGVICDVCSCELQKEERSSRCNFHGDFMCGHCVCHEGWSGKTCNCSTGSQSDLQPCRREGEDKVCSGRGECQCGHCVCYGEGRYEGQFCEYDNFQCPRTSGFLCNDRGRCSMGQCVCEPGWTGLSCDCPLSNATCIDSNGGLCNGRGHCECGRCHCNQQSLYTDTVCEINYSAIHLGLCEDLRSCVQCQAWGTGEKKGRTCEECSFKVKMVDELKKAEEVVEHCSFRDEDDDCTYSYTVEGDSAPGPNSTVLVQRRKECPPGTFWWLIPLLIFLLLFPVLLLLLCWKYCACCKACLALLPCCNRGHMVGFKEDHYMLRENLMASDHLDTPLLRSGNLKGRDTVRWKITNNVQRPGFASHAAGINPSELVPYGLSLRLARLCTENLLKPGTRECDQLRQEVEENLNEVYRQITGAHNLQQTKFRQQPNAGKKQDHTIVDTVLMAPRSAKQALLKLTEKHVEQGAFHELKVAPGYYTLTADQDARGMVEFQEGVELVDVRVPLFIRPEDDDEKQLLVEAIDVPVGTATLGRRLVNITIIKEQASGIVSFEQPEYLVSSGEHVARIPVVRRILDSGKSQVSYRTQDNTAKANRDYIPMEGELLFQPGETWKELQVKLLELQEMDSLLRGPQTRRFYIQLSNPKFGARLGQPQCATVITGDRDELDRNVMNQTVSSPPPPRGDLGAPQNPNAKAAGSRKIHFNWLPPPGKPTGYRVKYWIQGDSESEAHLLDSKVPSVELTNLYPYCDYEMKVCAYGAQGEGPYSSPVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYGLVNEDNRPIGPMKKVLVDSPKKRTLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDIPIVDAQSGEDYESFLMYSDDVLRSPAGSQRPSVSDDTGCGWKFEPLLGEELDLRRVTWRLPPELIPRLSAGSRRSSDSGPPGDGAADGADGGTRGASGEGEHLVNGRMDFAFPGSANSLHRMTVSTAAHGAHLSPQLSHRVLSTSSTLTRDYHSLTRTEHSHSATLPRDYSTLTSLSSQSLPPIWEHGRSRLPLSWALGSRSRAQMKGAPRSGDLRDSIILAGQPAAPSWGPGSRLAAGVPNTPTRLVFSALGPTSLKVSWQEPQCERALQGYSVEYQLLNGGELYRLNIPNPSQTSVVVEDLLPNHSYVFRVRAQSQEGWGPEREGVITIESQVHPQSPLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPDGDILGYLVTCEMAHGGEPATTFLVDGDSPESRLTVPGLSENVPYKFKVQAKTTQGFGPEREGIITIESQDGGPFPQLGGHLGLFQHPASGEYSSITTHSSTTEPFLLDGLTLGSQHLEASGSLTRHVTQEFVSRTLTTSGTLSTHVDQQFFQT

Target ORF information:

RefSeq Version	XM_024979795.1
Organism	Bos taurus(cattle)
Definition	Bos taurus integrin subunit beta 4 (ITGB4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979795.1

ORF Insert Sequence:

ATGGCAGGGC CACATCCCAG CCCGTGGACC AGGCTGCTGC TGGCAGCGCT GCTGAGCGTC 
AGCTTCCCCG GGCACATGGC GAACCGCTGC AAGAAGGCCC AGGTGAAGAG CTGTACCGAG 
TGCATCCGCG TGGACAAGGA CTGCGCCTAC TGCACGGACG AGGTGTTCAA GGAACGGCGC 
TGTAACACCC AGGCCGAGCT GCTGGCTGCC GGCTGCCGGC TGGAGAGCGT GGTGGTCATG 
GAGAGCAGCT TCGAGATCAC GGAGGAAAGG CAGATCGACA CCACGCTGCG CCGCAGCCAG 
GTGTCCCCCC AGGCGCTGCG GGTGCGGCTG CGGCCGGGCG AGGAGCGGCA CTTCGAGCTG 
CAGGTGTTCG AGCCCCTGGA GAGCCCCATG GACCTGTATA TCCTCATGGA CTTCTCCAAC 
TCCATGTCTG ATGATCTGGA CAACCTCAAG AAGATGGGGC AAGATCTGGC TCAGGTCCTG 
AGGCAGCTCA CCAGTGACTA CACAATAGGA TTTGGCAAGT TCGTGGACAA AGTCAGCGTC 
CCTCAGACAG ACATGAGGCC TGAGAAGCTG AAGGAACCCT GGCCCAACAG CGACCCCCCC 
TTCTCCTTCA AGAATGTCAT CAGCCTGACG GAAGATGTGG AGGAGTTCCG GAATAAGCTG 
AAGGGGGAGC GCATCTCAGG CAACCTGGAT GCCCCGGAAG GAGGTTTTGA TGCCATCCTG 
CAGACGGCCG TGTGCACCAG TGACATTGGC TGGCGCCCTG ACAGCACCCA CTTGCTGGTG 
TTCTCCACCG AGTCAGCCTT CCACTACGAG GCCGACGGGG CCAACGTGCT GGCCGGCATC 
ATGAGGCGTA ACGACGAGGA ATGCCACCTG GACCCCACGG GCACCTACAC CCAGTACAAG 
ATGCAGGACT ACCCGTCGGT GCCCACCCTG GTGCGCCTGC TCGGCCAACA CAACATCATC 
CCCATCTTCG CCGTCACCAA CTACTCCTAT AGCTACTATG AGAGGCTGTC GTCATATTTC 
CCCGTCTCTT CACTGGGGGT GCTGCAGGAG GACTCGTCCA ACATTGTGGA TCTGCTGCAG 
GAAGCCTTCA ACCGCATCCG CTCCAACCTG GACATTCGGG CCCTGGACAG TCCTCGAGGC 
CTGCGGACAG AGGTCACCTC TAGGATGTTC CAGAAGACGG ACACGGGGTC CTTTTTCATC 
CGGCGAGGGG AAGTGGGCAC ATACCAAGTG CAGCTCCGGG CCATCGAGGA CCTGGACGGG 
GCTCATGTGT GTCAGCTTCT GGAGGCAGAC CAGAAGGGCA CCATCCATCT GAAGCCATCC 
TTCTCCAACG GCCTCCGGAT GGACGTGGGC GTCATCTGTG ACGTGTGCTC CTGTGAGCTG 
CAAAAAGAGG AGCGGTCATC TCGCTGCAAC TTCCACGGGG ACTTCATGTG CGGACACTGT 
GTGTGCCACG AGGGCTGGAG CGGCAAGACC TGTAACTGCT CCACCGGCTC CCAGAGCGAC 
CTGCAGCCCT GCCGGCGGGA GGGCGAGGAC AAGGTGTGCT CCGGGCGGGG CGAGTGCCAG 
TGTGGCCACT GTGTGTGCTA TGGCGAAGGC CGCTACGAGG GTCAGTTCTG CGAATACGAC 
AACTTCCAGT GTCCCCGCAC CTCCGGGTTC CTCTGCAATG ACCGGGGACG CTGCTCCATG 
GGTCAGTGCG TGTGTGAGCC TGGCTGGACA GGCCTGAGCT GTGACTGTCC TCTCAGCAAT 
GCCACCTGCA TCGACAGTAA CGGGGGTCTC TGTAATGGGC GTGGCCACTG CGAGTGCGGC 
CGCTGCCACT GCAACCAACA GTCGCTCTAC ACGGACACCG TCTGCGAGAT CAACTACTCG 
GCGATCCACC TGGGCCTCTG TGAGGACCTG CGCTCCTGCG TGCAGTGCCA GGCCTGGGGC 
ACCGGTGAAA AGAAGGGGCG CACGTGCGAG GAGTGCAGCT TCAAGGTCAA GATGGTGGAT 
GAGCTCAAGA AAGCGGAGGA GGTGGTGGAG CACTGCTCCT TCCGGGACGA GGACGACGAC 
TGTACCTACA GTTACACCGT GGAGGGGGAC AGCGCCCCCG GGCCCAACAG CACCGTCTTG 
GTGCAGAGGA GGAAGGAATG CCCCCCGGGC ACCTTCTGGT GGCTCATCCC CCTGCTCATC 
TTCCTCCTCC TGTTCCCGGT CCTGCTGCTG CTGCTCTGCT GGAAGTACTG TGCCTGCTGC 
AAGGCCTGCC TGGCCCTTCT CCCTTGCTGC AACCGAGGTC ACATGGTGGG CTTCAAGGAA 
GACCATTACA TGCTGCGAGA GAACCTGATG GCCTCGGACC ACTTGGACAC GCCCTTGCTG 
CGCAGCGGGA ACCTCAAGGG TCGCGACACA GTCCGATGGA AGATCACCAA CAACGTGCAG 
CGGCCGGGCT TCGCCTCGCA CGCTGCAGGC ATCAACCCCT CAGAGCTGGT GCCCTACGGA 
CTGTCCCTGC GGCTTGCTCG CCTCTGCACC GAGAACCTGC TGAAGCCTGG CACGCGAGAA 
TGCGACCAGC TGCGCCAGGA GGTGGAGGAG AATCTGAACG AGGTGTACCG ACAAATCACA 
GGCGCTCACA ACCTCCAGCA GACCAAGTTC CGGCAGCAGC CCAACGCCGG GAAAAAGCAG 
GACCACACCA TTGTGGACAC TGTGCTGATG GCTCCCCGCT CGGCCAAGCA GGCCCTGCTG 
AAGCTGACGG AGAAGCACGT GGAGCAGGGG GCCTTCCATG AGCTCAAGGT GGCCCCCGGC 
TATTACACGC TCACCGCAGA CCAGGATGCC CGGGGCATGG TGGAATTCCA GGAGGGCGTG 
GAGCTGGTGG ACGTGCGGGT GCCTTTGTTT ATCCGGCCCG AGGACGACGA CGAGAAGCAG 
CTGCTGGTGG AGGCCATCGA CGTGCCTGTG GGCACCGCCA CCCTCGGACG TCGCCTGGTC 
AATATCACCA TCATCAAGGA GCAAGCCAGC GGGATCGTGT CCTTTGAGCA GCCCGAGTAC 
TTGGTCAGCA GCGGGGAGCA CGTTGCCCGC ATCCCCGTGG TCCGGCGCAT CCTGGACAGC 
GGCAAATCAC AGGTCTCCTA CCGCACACAG GATAACACCG CCAAGGCCAA CCGGGACTAC 
ATCCCCATGG AGGGTGAGCT GCTGTTCCAA CCTGGGGAGA CCTGGAAGGA GCTGCAGGTG 
AAGCTCCTGG AGCTGCAGGA GATGGACTCC CTTCTGCGGG GCCCCCAGAC CCGCCGCTTC 
TACATCCAAC TCAGCAACCC CAAGTTCGGG GCCCGCCTGG GCCAGCCCCA GTGTGCCACT 
GTCATCACTG GGGACCGAGA CGAGCTGGAC CGGAATGTAA TGAACCAGAC CGTGTCATCA 
CCACCACCGC CCCGGGGTGA CCTGGGTGCC CCACAGAACC CCAATGCCAA GGCTGCTGGG 
TCCCGGAAGA TCCACTTCAA CTGGCTGCCT CCTCCTGGCA AGCCGACAGG GTACCGGGTG 
AAATATTGGA TCCAGGGTGA CTCCGAGTCT GAAGCCCACC TGCTTGACAG CAAGGTGCCC 
TCAGTGGAGC TCACCAACCT GTACCCATAT TGTGACTACG AGATGAAGGT GTGCGCCTAT 
GGGGCGCAGG GCGAGGGCCC CTACAGCTCC CCGGTGTCCT GTCGCACCCA CCAGGAAGTG 
CCCAGCGAGC CCGGGCGTCT GGCCTTCAAC GTCGTCTCCT CCACCGTGAC CCAGCTGAGC 
TGGGCCGAGC CGGCTGAGAC CAACGGCGAG ATCACAGCCT ACGAGGTCTG CTATGGCCTG 
GTCAATGAGG ACAACCGACC CATCGGGCCC ATGAAGAAGG TGCTGGTGGA CAGCCCCAAG 
AAGCGGACGC TGCTCATCGA GAACCTGCGC GAGTCCCAGC CATACCGCTA CACGGTCAAG 
GCTCGCAACG GGGCAGGCTG GGGGCCTGAG CGGGAGGCCA TCATCAACCT GGCCACCCAG 
CCCAAGCGAC CCATGTCCAT CCCCATCATC CCTGACATCC CCATCGTGGA CGCCCAGAGT 
GGGGAGGACT ATGAGAGCTT CCTCATGTAC AGCGATGATG TGCTCCGCTC TCCAGCCGGC 
AGCCAGAGGC CCAGCGTCTC TGATGACACC GGCTGCGGCT GGAAGTTCGA GCCCCTGCTG 
GGGGAGGAGC TGGACCTGAG GCGCGTCACG TGGCGGCTGC CCCCGGAGCT CATCCCGCGC 
CTGTCCGCCG GCAGCCGGCG CTCCTCCGAC TCCGGGCCCC CGGGAGACGG CGCCGCCGAT 
GGGGCCGATG GGGGCACGCG GGGCGCCAGC GGGGAGGGAG AGCACCTGGT GAACGGGCGG 
ATGGACTTTG CCTTCCCGGG CAGCGCCAAC TCCCTGCACA GGATGACCGT GAGCACCGCG 
GCCCACGGCG CCCACCTGAG CCCGCAGTTG TCCCACCGCG TGCTGAGCAC CTCGTCCACC 
CTCACGCGGG ACTACCACTC GCTGACCCGC ACGGAGCACT CGCACTCCGC CACGCTGCCC 
AGGGACTACT CCACTCTCAC CTCCCTTTCC TCCCAAAGCC TCCCTCCCAT CTGGGAACAC 
GGGAGGAGCA GGCTTCCGCT GTCCTGGGCC CTAGGGTCCC GGAGTCGGGC TCAGATGAAG 
GGAGCCCCCC GCTCCGGGGA CTTGAGAGAC TCTATAATCC TGGCTGGGCA GCCAGCAGCG 
CCCTCTTGGG GCCCAGGCTC CCGCTTGGCT GCGGGTGTGC CCAACACACC CACCCGCCTG 
GTGTTCTCCG CCCTTGGACC CACGTCTCTG AAAGTGAGCT GGCAGGAGCC GCAGTGTGAG 
CGTGCGCTGC AGGGCTACAG TGTGGAGTAC CAGCTGCTGA ACGGCGGTGA GCTGTATCGC 
CTCAACATCC CCAACCCCAG CCAGACCTCG GTGGTGGTGG AGGACCTCCT GCCCAACCAC 
TCCTACGTGT TCCGTGTGCG GGCCCAGAGC CAGGAGGGCT GGGGCCCGGA GCGTGAGGGT 
GTCATCACCA TTGAGTCACA GGTGCACCCG CAGAGCCCGC TCTGCCCCCT GCCGGGCTCC 
GCCTTCACTT TGAGCACACC CAGCGCCCCG GGCCCGCTGG TGTTCACTGC CCTGAGCCCC 
GACTCGCTGC AGCTGAGCTG GGAGCGGCCC CGCAGGCCAG ATGGCGACAT TCTGGGCTAC 
CTGGTGACGT GTGAGATGGC CCACGGAGGA GAGCCAGCCA CTACGTTCCT GGTTGACGGC 
GACAGCCCTG AGAGCCGGCT GACCGTGCCT GGCCTCAGCG AGAACGTGCC CTACAAGTTC 
AAGGTGCAGG CCAAGACCAC CCAAGGCTTC GGGCCGGAGC GAGAGGGAAT CATCACCATC 
GAGTCCCAGG ATGGAGGACC CTTTCCACAG CTGGGCGGCC ACTTGGGGCT CTTCCAGCAC 
CCAGCGTCAG GGGAATACAG CAGCATCACC ACCCACTCCA GCACCACTGA ACCCTTCCTA 
CTGGATGGAC TGACCCTGGG CTCCCAGCAT CTGGAAGCAA GTGGCTCCCT CACCCGGCAT 
GTGACACAGG AGTTTGTGAG CCGGACTCTG ACCACCAGTG GGACCCTCAG CACCCACGTG 
GACCAACAGT TCTTCCAGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITGB4 ( XM_024979796.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835564.1 Bos taurus integrin subunit beta 4 (ITGB4), transcript variant X2, mRNA.

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CloneID	OBa226110
Clone ID Related Accession (Same CDS sequence)	XM_024979796.1
Accession Version	XM_024979796.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	integrin beta-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGC CACATCCCAG CCCGTGGACC AGGCTGCTGC TGGCAGCGCT GCTGAGCGTC 
AGCTTCCCCG GGCACATGGC GAACCGCTGC AAGAAGGCCC AGGTGAAGAG CTGTACCGAG 
TGCATCCGCG TGGACAAGGA CTGCGCCTAC TGCACGGACG AGGTGTTCAA GGAACGGCGC 
TGTAACACCC AGGCCGAGCT GCTGGCTGCC GGCTGCCGGC TGGAGAGCGT GGTGGTCATG 
GAGAGCAGCT TCGAGATCAC GGAGGAAAGG CAGATCGACA CCACGCTGCG CCGCAGCCAG 
GTGTCCCCCC AGGCGCTGCG GGTGCGGCTG CGGCCGGGCG AGGAGCGGCA CTTCGAGCTG 
CAGGTGTTCG AGCCCCTGGA GAGCCCCATG GACCTGTATA TCCTCATGGA CTTCTCCAAC 
TCCATGTCTG ATGATCTGGA CAACCTCAAG AAGATGGGGC AAGATCTGGC TCAGGTCCTG 
AGGCAGCTCA CCAGTGACTA CACAATAGGA TTTGGCAAGT TCGTGGACAA AGTCAGCGTC 
CCTCAGACAG ACATGAGGCC TGAGAAGCTG AAGGAACCCT GGCCCAACAG CGACCCCCCC 
TTCTCCTTCA AGAATGTCAT CAGCCTGACG GAAGATGTGG AGGAGTTCCG GAATAAGCTG 
AAGGGGGAGC GCATCTCAGG CAACCTGGAT GCCCCGGAAG GAGGTTTTGA TGCCATCCTG 
CAGACGGCCG TGTGCACCAG TGACATTGGC TGGCGCCCTG ACAGCACCCA CTTGCTGGTG 
TTCTCCACCG AGTCAGCCTT CCACTACGAG GCCGACGGGG CCAACGTGCT GGCCGGCATC 
ATGAGGCGTA ACGACGAGGA ATGCCACCTG GACCCCACGG GCACCTACAC CCAGTACAAG 
ATGCAGGACT ACCCGTCGGT GCCCACCCTG GTGCGCCTGC TCGGCCAACA CAACATCATC 
CCCATCTTCG CCGTCACCAA CTACTCCTAT AGCTACTATG AGAGGCTGTC GTCATATTTC 
CCCGTCTCTT CACTGGGGGT GCTGCAGGAG GACTCGTCCA ACATTGTGGA TCTGCTGCAG 
GAAGCCTTCA ACCGCATCCG CTCCAACCTG GACATTCGGG CCCTGGACAG TCCTCGAGGC 
CTGCGGACAG AGGTCACCTC TAGGATGTTC CAGAAGACGG ACACGGGGTC CTTTTTCATC 
CGGCGAGGGG AAGTGGGCAC ATACCAAGTG CAGCTCCGGG CCATCGAGGA CCTGGACGGG 
GCTCATGTGT GTCAGCTTCT GGAGGCAGAC CAGAAGGGCA CCATCCATCT GAAGCCATCC 
TTCTCCAACG GCCTCCGGAT GGACGTGGGC GTCATCTGTG ACGTGTGCTC CTGTGAGCTG 
CAAAAAGAGG AGCGGTCATC TCGCTGCAAC TTCCACGGGG ACTTCATGTG CGGACACTGT 
GTGTGCCACG AGGGCTGGAG CGGCAAGACC TGTAACTGCT CCACCGGCTC CCAGAGCGAC 
CTGCAGCCCT GCCGGCGGGA GGGCGAGGAC AAGGTGTGCT CCGGGCGGGG CGAGTGCCAG 
TGTGGCCACT GTGTGTGCTA TGGCGAAGGC CGCTACGAGG GTCAGTTCTG CGAATACGAC 
AACTTCCAGT GTCCCCGCAC CTCCGGGTTC CTCTGCAATG ACCGGGGACG CTGCTCCATG 
GGTCAGTGCG TGTGTGAGCC TGGCTGGACA GGCCTGAGCT GTGACTGTCC TCTCAGCAAT 
GCCACCTGCA TCGACAGTAA CGGGGGTCTC TGTAATGGGC GTGGCCACTG CGAGTGCGGC 
CGCTGCCACT GCAACCAACA GTCGCTCTAC ACGGACACCG TCTGCGAGAT CAACTACTCG 
GCGATCCACC TGGGCCTCTG TGAGGACCTG CGCTCCTGCG TGCAGTGCCA GGCCTGGGGC 
ACCGGTGAAA AGAAGGGGCG CACGTGCGAG GAGTGCAGCT TCAAGGTCAA GATGGTGGAT 
GAGCTCAAGA AAGCGGAGGA GGTGGTGGAG CACTGCTCCT TCCGGGACGA GGACGACGAC 
TGTACCTACA GTTACACCGT GGAGGGGGAC AGCGCCCCCG GGCCCAACAG CACCGTCTTG 
GTGCAGAGGA GGAAGGAATG CCCCCCGGGC ACCTTCTGGT GGCTCATCCC CCTGCTCATC 
TTCCTCCTCC TGTTCCCGGT CCTGCTGCTG CTGCTCTGCT GGAAGTACTG TGCCTGCTGC 
AAGGCCTGCC TGGCCCTTCT CCCTTGCTGC AACCGAGGTC ACATGGTGGG CTTCAAGGAA 
GACCATTACA TGCTGCGAGA GAACCTGATG GCCTCGGACC ACTTGGACAC GCCCTTGCTG 
CGCAGCGGGA ACCTCAAGGG TCGCGACACA GTCCGATGGA AGATCACCAA CAACGTGCAG 
CGGCCGGGCT TCGCCTCGCA CGCTGCAGGC ATCAACCCCT CAGAGCTGGT GCCCTACGGA 
CTGTCCCTGC GGCTTGCTCG CCTCTGCACC GAGAACCTGC TGAAGCCTGG CACGCGAGAA 
TGCGACCAGC TGCGCCAGGA GGTGGAGGAG AATCTGAACG AGGTGTACCG ACAAATCACA 
GGCGCTCACA ACCTCCAGCA GACCAAGTTC CGGCAGCAGC CCAACGCCGG GAAAAAGCAG 
GACCACACCA TTGTGGACAC TGTGCTGATG GCTCCCCGCT CGGCCAAGCA GGCCCTGCTG 
AAGCTGACGG AGAAGCACGT GGAGCAGGGG GCCTTCCATG AGCTCAAGGT GGCCCCCGGC 
TATTACACGC TCACCGCAGA CCAGGATGCC CGGGGCATGG TGGAATTCCA GGAGGGCGTG 
GAGCTGGTGG ACGTGCGGGT GCCTTTGTTT ATCCGGCCCG AGGACGACGA CGAGAAGCAG 
CTGCTGGTGG AGGCCATCGA CGTGCCTGTG GGCACCGCCA CCCTCGGACG TCGCCTGGTC 
AATATCACCA TCATCAAGGA GCAAGCCAGC GGGATCGTGT CCTTTGAGCA GCCCGAGTAC 
TTGGTCAGCA GCGGGGAGCA CGTTGCCCGC ATCCCCGTGG TCCGGCGCAT CCTGGACAGC 
GGCAAATCAC AGGTCTCCTA CCGCACACAG GATAACACCG CCAAGGCCAA CCGGGACTAC 
ATCCCCATGG AGGGTGAGCT GCTGTTCCAA CCTGGGGAGA CCTGGAAGGA GCTGCAGGTG 
AAGCTCCTGG AGCTGCAGGA GATGGACTCC CTTCTGCGGG GCCCCCAGAC CCGCCGCTTC 
TACATCCAAC TCAGCAACCC CAAGTTCGGG GCCCGCCTGG GCCAGCCCCA GTGTGCCACT 
GTCATCACTG GGGACCGAGA CGAGCTGGAC CGGAATGTAA TGAACCAGAC CGTGTCATCA 
CCACCACCGC CCCGGGGTGA CCTGGGTGCC CCACAGAACC CCAATGCCAA GGCTGCTGGG 
TCCCGGAAGA TCCACTTCAA CTGGCTGCCT CCTCCTGGCA AGCCGACAGG GTACCGGGTG 
AAATATTGGA TCCAGGGTGA CTCCGAGTCT GAAGCCCACC TGCTTGACAG CAAGGTGCCC 
TCAGTGGAGC TCACCAACCT GTACCCATAT TGTGACTACG AGATGAAGGT GTGCGCCTAT 
GGGGCGCAGG GCGAGGGCCC CTACAGCTCC CCGGTGTCCT GTCGCACCCA CCAGGAAGTG 
CCCAGCGAGC CCGGGCGTCT GGCCTTCAAC GTCGTCTCCT CCACCGTGAC CCAGCTGAGC 
TGGGCCGAGC CGGCTGAGAC CAACGGCGAG ATCACAGCCT ACGAGGTCTG CTATGGCCTG 
GTCAATGAGG ACAACCGACC CATCGGGCCC ATGAAGAAGG TGCTGGTGGA CAGCCCCAAG 
AAGCGGACGC TGCTCATCGA GAACCTGCGC GAGTCCCAGC CATACCGCTA CACGGTCAAG 
GCTCGCAACG GGGCAGGCTG GGGGCCTGAG CGGGAGGCCA TCATCAACCT GGCCACCCAG 
CCCAAGCGAC CCATGTCCAT CCCCATCATC CCTGACATCC CCATCGTGGA CGCCCAGAGT 
GGGGAGGACT ATGAGAGCTT CCTCATGTAC AGCGATGATG TGCTCCGCTC TCCAGCCGGC 
AGCCAGAGGC CCAGCGTCTC TGATGACACC GGCTGCGGCT GGAAGTTCGA GCCCCTGCTG 
GGGGAGGAGC TGGACCTGAG GCGCGTCACG TGGCGGCTGC CCCCGGAGCT CATCCCGCGC 
CTGTCCGCCG GCAGCCGGCG CTCCTCCGAC TCCGGGCCCC CGGGAGACGG CGCCGCCGAT 
GGGGCCGATG GGGGCACGCG GGGCGCCAGC GGGGAGGGAG AGCACCTGGT GAACGGGCGG 
ATGGACTTTG CCTTCCCGGG CAGCGCCAAC TCCCTGCACA GGATGACCGT GAGCACCGCG 
GCCCACGGCG CCCACCTGAG CCCGCAGTTG TCCCACCGCG TGCTGAGCAC CTCGTCCACC 
CTCACGCGGG ACTACCACTC GCTGACCCGC ACGGAGCACT CGCACTCCGC CACGCTGCCC 
AGGGACTACT CCACTCTCAC CTCCCTTTCC TCCCAAAGCT CCCGCTTGGC TGCGGGTGTG 
CCCAACACAC CCACCCGCCT GGTGTTCTCC GCCCTTGGAC CCACGTCTCT GAAAGTGAGC 
TGGCAGGAGC CGCAGTGTGA GCGTGCGCTG CAGGGCTACA GTGTGGAGTA CCAGCTGCTG 
AACGGCGGTG AGCTGTATCG CCTCAACATC CCCAACCCCA GCCAGACCTC GGTGGTGGTG 
GAGGACCTCC TGCCCAACCA CTCCTACGTG TTCCGTGTGC GGGCCCAGAG CCAGGAGGGC 
TGGGGCCCGG AGCGTGAGGG TGTCATCACC ATTGAGTCAC AGGTGCACCC GCAGAGCCCG 
CTCTGCCCCC TGCCGGGCTC CGCCTTCACT TTGAGCACAC CCAGCGCCCC GGGCCCGCTG 
GTGTTCACTG CCCTGAGCCC CGACTCGCTG CAGCTGAGCT GGGAGCGGCC CCGCAGGCCA 
GATGGCGACA TTCTGGGCTA CCTGGTGACG TGTGAGATGG CCCACGGAGG AGAGCCAGCC 
ACTACGTTCC TGGTTGACGG CGACAGCCCT GAGAGCCGGC TGACCGTGCC TGGCCTCAGC 
GAGAACGTGC CCTACAAGTT CAAGGTGCAG GCCAAGACCA CCCAAGGCTT CGGGCCGGAG 
CGAGAGGGAA TCATCACCAT CGAGTCCCAG GATGGAGGAC CCTTTCCACA GCTGGGCGGC 
CACTTGGGGC TCTTCCAGCA CCCAGCGTCA GGGGAATACA GCAGCATCAC CACCCACTCC 
AGCACCACTG AACCCTTCCT ACTGGATGGA CTGACCCTGG GCTCCCAGCA TCTGGAAGCA 
AGTGGCTCCC TCACCCGGCA TGTGACACAG GAGTTTGTGA GCCGGACTCT GACCACCAGT 
GGGACCCTCA GCACCCACGT GGACCAACAG TTCTTCCAGA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835564.1
CDS	230..5674
Translation	MAGPHPSPWTRLLLAALLSVSFPGHMANRCKKAQVKSCTECIRVDKDCAYCTDEVFKERRCNTQAELLAAGCRLESVVVMESSFEITEERQIDTTLRRSQVSPQALRVRLRPGEERHFELQVFEPLESPMDLYILMDFSNSMSDDLDNLKKMGQDLAQVLRQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTEDVEEFRNKLKGERISGNLDAPEGGFDAILQTAVCTSDIGWRPDSTHLLVFSTESAFHYEADGANVLAGIMRRNDEECHLDPTGTYTQYKMQDYPSVPTLVRLLGQHNIIPIFAVTNYSYSYYERLSSYFPVSSLGVLQEDSSNIVDLLQEAFNRIRSNLDIRALDSPRGLRTEVTSRMFQKTDTGSFFIRRGEVGTYQVQLRAIEDLDGAHVCQLLEADQKGTIHLKPSFSNGLRMDVGVICDVCSCELQKEERSSRCNFHGDFMCGHCVCHEGWSGKTCNCSTGSQSDLQPCRREGEDKVCSGRGECQCGHCVCYGEGRYEGQFCEYDNFQCPRTSGFLCNDRGRCSMGQCVCEPGWTGLSCDCPLSNATCIDSNGGLCNGRGHCECGRCHCNQQSLYTDTVCEINYSAIHLGLCEDLRSCVQCQAWGTGEKKGRTCEECSFKVKMVDELKKAEEVVEHCSFRDEDDDCTYSYTVEGDSAPGPNSTVLVQRRKECPPGTFWWLIPLLIFLLLFPVLLLLLCWKYCACCKACLALLPCCNRGHMVGFKEDHYMLRENLMASDHLDTPLLRSGNLKGRDTVRWKITNNVQRPGFASHAAGINPSELVPYGLSLRLARLCTENLLKPGTRECDQLRQEVEENLNEVYRQITGAHNLQQTKFRQQPNAGKKQDHTIVDTVLMAPRSAKQALLKLTEKHVEQGAFHELKVAPGYYTLTADQDARGMVEFQEGVELVDVRVPLFIRPEDDDEKQLLVEAIDVPVGTATLGRRLVNITIIKEQASGIVSFEQPEYLVSSGEHVARIPVVRRILDSGKSQVSYRTQDNTAKANRDYIPMEGELLFQPGETWKELQVKLLELQEMDSLLRGPQTRRFYIQLSNPKFGARLGQPQCATVITGDRDELDRNVMNQTVSSPPPPRGDLGAPQNPNAKAAGSRKIHFNWLPPPGKPTGYRVKYWIQGDSESEAHLLDSKVPSVELTNLYPYCDYEMKVCAYGAQGEGPYSSPVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYGLVNEDNRPIGPMKKVLVDSPKKRTLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDIPIVDAQSGEDYESFLMYSDDVLRSPAGSQRPSVSDDTGCGWKFEPLLGEELDLRRVTWRLPPELIPRLSAGSRRSSDSGPPGDGAADGADGGTRGASGEGEHLVNGRMDFAFPGSANSLHRMTVSTAAHGAHLSPQLSHRVLSTSSTLTRDYHSLTRTEHSHSATLPRDYSTLTSLSSQSSRLAAGVPNTPTRLVFSALGPTSLKVSWQEPQCERALQGYSVEYQLLNGGELYRLNIPNPSQTSVVVEDLLPNHSYVFRVRAQSQEGWGPEREGVITIESQVHPQSPLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPDGDILGYLVTCEMAHGGEPATTFLVDGDSPESRLTVPGLSENVPYKFKVQAKTTQGFGPEREGIITIESQDGGPFPQLGGHLGLFQHPASGEYSSITTHSSTTEPFLLDGLTLGSQHLEASGSLTRHVTQEFVSRTLTTSGTLSTHVDQQFFQT

Target ORF information:

RefSeq Version	XM_024979796.1
Organism	Bos taurus(cattle)
Definition	Bos taurus integrin subunit beta 4 (ITGB4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979796.1

ORF Insert Sequence:

ATGGCAGGGC CACATCCCAG CCCGTGGACC AGGCTGCTGC TGGCAGCGCT GCTGAGCGTC 
AGCTTCCCCG GGCACATGGC GAACCGCTGC AAGAAGGCCC AGGTGAAGAG CTGTACCGAG 
TGCATCCGCG TGGACAAGGA CTGCGCCTAC TGCACGGACG AGGTGTTCAA GGAACGGCGC 
TGTAACACCC AGGCCGAGCT GCTGGCTGCC GGCTGCCGGC TGGAGAGCGT GGTGGTCATG 
GAGAGCAGCT TCGAGATCAC GGAGGAAAGG CAGATCGACA CCACGCTGCG CCGCAGCCAG 
GTGTCCCCCC AGGCGCTGCG GGTGCGGCTG CGGCCGGGCG AGGAGCGGCA CTTCGAGCTG 
CAGGTGTTCG AGCCCCTGGA GAGCCCCATG GACCTGTATA TCCTCATGGA CTTCTCCAAC 
TCCATGTCTG ATGATCTGGA CAACCTCAAG AAGATGGGGC AAGATCTGGC TCAGGTCCTG 
AGGCAGCTCA CCAGTGACTA CACAATAGGA TTTGGCAAGT TCGTGGACAA AGTCAGCGTC 
CCTCAGACAG ACATGAGGCC TGAGAAGCTG AAGGAACCCT GGCCCAACAG CGACCCCCCC 
TTCTCCTTCA AGAATGTCAT CAGCCTGACG GAAGATGTGG AGGAGTTCCG GAATAAGCTG 
AAGGGGGAGC GCATCTCAGG CAACCTGGAT GCCCCGGAAG GAGGTTTTGA TGCCATCCTG 
CAGACGGCCG TGTGCACCAG TGACATTGGC TGGCGCCCTG ACAGCACCCA CTTGCTGGTG 
TTCTCCACCG AGTCAGCCTT CCACTACGAG GCCGACGGGG CCAACGTGCT GGCCGGCATC 
ATGAGGCGTA ACGACGAGGA ATGCCACCTG GACCCCACGG GCACCTACAC CCAGTACAAG 
ATGCAGGACT ACCCGTCGGT GCCCACCCTG GTGCGCCTGC TCGGCCAACA CAACATCATC 
CCCATCTTCG CCGTCACCAA CTACTCCTAT AGCTACTATG AGAGGCTGTC GTCATATTTC 
CCCGTCTCTT CACTGGGGGT GCTGCAGGAG GACTCGTCCA ACATTGTGGA TCTGCTGCAG 
GAAGCCTTCA ACCGCATCCG CTCCAACCTG GACATTCGGG CCCTGGACAG TCCTCGAGGC 
CTGCGGACAG AGGTCACCTC TAGGATGTTC CAGAAGACGG ACACGGGGTC CTTTTTCATC 
CGGCGAGGGG AAGTGGGCAC ATACCAAGTG CAGCTCCGGG CCATCGAGGA CCTGGACGGG 
GCTCATGTGT GTCAGCTTCT GGAGGCAGAC CAGAAGGGCA CCATCCATCT GAAGCCATCC 
TTCTCCAACG GCCTCCGGAT GGACGTGGGC GTCATCTGTG ACGTGTGCTC CTGTGAGCTG 
CAAAAAGAGG AGCGGTCATC TCGCTGCAAC TTCCACGGGG ACTTCATGTG CGGACACTGT 
GTGTGCCACG AGGGCTGGAG CGGCAAGACC TGTAACTGCT CCACCGGCTC CCAGAGCGAC 
CTGCAGCCCT GCCGGCGGGA GGGCGAGGAC AAGGTGTGCT CCGGGCGGGG CGAGTGCCAG 
TGTGGCCACT GTGTGTGCTA TGGCGAAGGC CGCTACGAGG GTCAGTTCTG CGAATACGAC 
AACTTCCAGT GTCCCCGCAC CTCCGGGTTC CTCTGCAATG ACCGGGGACG CTGCTCCATG 
GGTCAGTGCG TGTGTGAGCC TGGCTGGACA GGCCTGAGCT GTGACTGTCC TCTCAGCAAT 
GCCACCTGCA TCGACAGTAA CGGGGGTCTC TGTAATGGGC GTGGCCACTG CGAGTGCGGC 
CGCTGCCACT GCAACCAACA GTCGCTCTAC ACGGACACCG TCTGCGAGAT CAACTACTCG 
GCGATCCACC TGGGCCTCTG TGAGGACCTG CGCTCCTGCG TGCAGTGCCA GGCCTGGGGC 
ACCGGTGAAA AGAAGGGGCG CACGTGCGAG GAGTGCAGCT TCAAGGTCAA GATGGTGGAT 
GAGCTCAAGA AAGCGGAGGA GGTGGTGGAG CACTGCTCCT TCCGGGACGA GGACGACGAC 
TGTACCTACA GTTACACCGT GGAGGGGGAC AGCGCCCCCG GGCCCAACAG CACCGTCTTG 
GTGCAGAGGA GGAAGGAATG CCCCCCGGGC ACCTTCTGGT GGCTCATCCC CCTGCTCATC 
TTCCTCCTCC TGTTCCCGGT CCTGCTGCTG CTGCTCTGCT GGAAGTACTG TGCCTGCTGC 
AAGGCCTGCC TGGCCCTTCT CCCTTGCTGC AACCGAGGTC ACATGGTGGG CTTCAAGGAA 
GACCATTACA TGCTGCGAGA GAACCTGATG GCCTCGGACC ACTTGGACAC GCCCTTGCTG 
CGCAGCGGGA ACCTCAAGGG TCGCGACACA GTCCGATGGA AGATCACCAA CAACGTGCAG 
CGGCCGGGCT TCGCCTCGCA CGCTGCAGGC ATCAACCCCT CAGAGCTGGT GCCCTACGGA 
CTGTCCCTGC GGCTTGCTCG CCTCTGCACC GAGAACCTGC TGAAGCCTGG CACGCGAGAA 
TGCGACCAGC TGCGCCAGGA GGTGGAGGAG AATCTGAACG AGGTGTACCG ACAAATCACA 
GGCGCTCACA ACCTCCAGCA GACCAAGTTC CGGCAGCAGC CCAACGCCGG GAAAAAGCAG 
GACCACACCA TTGTGGACAC TGTGCTGATG GCTCCCCGCT CGGCCAAGCA GGCCCTGCTG 
AAGCTGACGG AGAAGCACGT GGAGCAGGGG GCCTTCCATG AGCTCAAGGT GGCCCCCGGC 
TATTACACGC TCACCGCAGA CCAGGATGCC CGGGGCATGG TGGAATTCCA GGAGGGCGTG 
GAGCTGGTGG ACGTGCGGGT GCCTTTGTTT ATCCGGCCCG AGGACGACGA CGAGAAGCAG 
CTGCTGGTGG AGGCCATCGA CGTGCCTGTG GGCACCGCCA CCCTCGGACG TCGCCTGGTC 
AATATCACCA TCATCAAGGA GCAAGCCAGC GGGATCGTGT CCTTTGAGCA GCCCGAGTAC 
TTGGTCAGCA GCGGGGAGCA CGTTGCCCGC ATCCCCGTGG TCCGGCGCAT CCTGGACAGC 
GGCAAATCAC AGGTCTCCTA CCGCACACAG GATAACACCG CCAAGGCCAA CCGGGACTAC 
ATCCCCATGG AGGGTGAGCT GCTGTTCCAA CCTGGGGAGA CCTGGAAGGA GCTGCAGGTG 
AAGCTCCTGG AGCTGCAGGA GATGGACTCC CTTCTGCGGG GCCCCCAGAC CCGCCGCTTC 
TACATCCAAC TCAGCAACCC CAAGTTCGGG GCCCGCCTGG GCCAGCCCCA GTGTGCCACT 
GTCATCACTG GGGACCGAGA CGAGCTGGAC CGGAATGTAA TGAACCAGAC CGTGTCATCA 
CCACCACCGC CCCGGGGTGA CCTGGGTGCC CCACAGAACC CCAATGCCAA GGCTGCTGGG 
TCCCGGAAGA TCCACTTCAA CTGGCTGCCT CCTCCTGGCA AGCCGACAGG GTACCGGGTG 
AAATATTGGA TCCAGGGTGA CTCCGAGTCT GAAGCCCACC TGCTTGACAG CAAGGTGCCC 
TCAGTGGAGC TCACCAACCT GTACCCATAT TGTGACTACG AGATGAAGGT GTGCGCCTAT 
GGGGCGCAGG GCGAGGGCCC CTACAGCTCC CCGGTGTCCT GTCGCACCCA CCAGGAAGTG 
CCCAGCGAGC CCGGGCGTCT GGCCTTCAAC GTCGTCTCCT CCACCGTGAC CCAGCTGAGC 
TGGGCCGAGC CGGCTGAGAC CAACGGCGAG ATCACAGCCT ACGAGGTCTG CTATGGCCTG 
GTCAATGAGG ACAACCGACC CATCGGGCCC ATGAAGAAGG TGCTGGTGGA CAGCCCCAAG 
AAGCGGACGC TGCTCATCGA GAACCTGCGC GAGTCCCAGC CATACCGCTA CACGGTCAAG 
GCTCGCAACG GGGCAGGCTG GGGGCCTGAG CGGGAGGCCA TCATCAACCT GGCCACCCAG 
CCCAAGCGAC CCATGTCCAT CCCCATCATC CCTGACATCC CCATCGTGGA CGCCCAGAGT 
GGGGAGGACT ATGAGAGCTT CCTCATGTAC AGCGATGATG TGCTCCGCTC TCCAGCCGGC 
AGCCAGAGGC CCAGCGTCTC TGATGACACC GGCTGCGGCT GGAAGTTCGA GCCCCTGCTG 
GGGGAGGAGC TGGACCTGAG GCGCGTCACG TGGCGGCTGC CCCCGGAGCT CATCCCGCGC 
CTGTCCGCCG GCAGCCGGCG CTCCTCCGAC TCCGGGCCCC CGGGAGACGG CGCCGCCGAT 
GGGGCCGATG GGGGCACGCG GGGCGCCAGC GGGGAGGGAG AGCACCTGGT GAACGGGCGG 
ATGGACTTTG CCTTCCCGGG CAGCGCCAAC TCCCTGCACA GGATGACCGT GAGCACCGCG 
GCCCACGGCG CCCACCTGAG CCCGCAGTTG TCCCACCGCG TGCTGAGCAC CTCGTCCACC 
CTCACGCGGG ACTACCACTC GCTGACCCGC ACGGAGCACT CGCACTCCGC CACGCTGCCC 
AGGGACTACT CCACTCTCAC CTCCCTTTCC TCCCAAAGCT CCCGCTTGGC TGCGGGTGTG 
CCCAACACAC CCACCCGCCT GGTGTTCTCC GCCCTTGGAC CCACGTCTCT GAAAGTGAGC 
TGGCAGGAGC CGCAGTGTGA GCGTGCGCTG CAGGGCTACA GTGTGGAGTA CCAGCTGCTG 
AACGGCGGTG AGCTGTATCG CCTCAACATC CCCAACCCCA GCCAGACCTC GGTGGTGGTG 
GAGGACCTCC TGCCCAACCA CTCCTACGTG TTCCGTGTGC GGGCCCAGAG CCAGGAGGGC 
TGGGGCCCGG AGCGTGAGGG TGTCATCACC ATTGAGTCAC AGGTGCACCC GCAGAGCCCG 
CTCTGCCCCC TGCCGGGCTC CGCCTTCACT TTGAGCACAC CCAGCGCCCC GGGCCCGCTG 
GTGTTCACTG CCCTGAGCCC CGACTCGCTG CAGCTGAGCT GGGAGCGGCC CCGCAGGCCA 
GATGGCGACA TTCTGGGCTA CCTGGTGACG TGTGAGATGG CCCACGGAGG AGAGCCAGCC 
ACTACGTTCC TGGTTGACGG CGACAGCCCT GAGAGCCGGC TGACCGTGCC TGGCCTCAGC 
GAGAACGTGC CCTACAAGTT CAAGGTGCAG GCCAAGACCA CCCAAGGCTT CGGGCCGGAG 
CGAGAGGGAA TCATCACCAT CGAGTCCCAG GATGGAGGAC CCTTTCCACA GCTGGGCGGC 
CACTTGGGGC TCTTCCAGCA CCCAGCGTCA GGGGAATACA GCAGCATCAC CACCCACTCC 
AGCACCACTG AACCCTTCCT ACTGGATGGA CTGACCCTGG GCTCCCAGCA TCTGGAAGCA 
AGTGGCTCCC TCACCCGGCA TGTGACACAG GAGTTTGTGA GCCGGACTCT GACCACCAGT 
GGGACCCTCA GCACCCACGT GGACCAACAG TTCTTCCAGA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITGB4 ( XM_024979797.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835565.1 Bos taurus integrin subunit beta 4 (ITGB4), transcript variant X3, mRNA.

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CloneID	OBa226111
Clone ID Related Accession (Same CDS sequence)	XM_024979797.1
Accession Version	XM_024979797.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5415bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	integrin beta-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGC CACATCCCAG CCCGTGGACC AGGCTGCTGC TGGCAGCGCT GCTGAGCGTC 
AGCTTCCCCG GGCACATGGC GAACCGCTGC AAGAAGGCCC AGGTGAAGAG CTGTACCGAG 
TGCATCCGCG TGGACAAGGA CTGCGCCTAC TGCACGGACG AGGTGTTCAA GGAACGGCGC 
TGTAACACCC AGGCCGAGCT GCTGGCTGCC GGCTGCCGGC TGGAGAGCGT GGTGGTCATG 
GAGAGCAGCT TCGAGATCAC GGAGGAAAGG CAGATCGACA CCACGCTGCG CCGCAGCCAG 
GTGTCCCCCC AGGCGCTGCG GGTGCGGCTG CGGCCGGGCG AGGAGCGGCA CTTCGAGCTG 
CAGGTGTTCG AGCCCCTGGA GAGCCCCATG GACCTGTATA TCCTCATGGA CTTCTCCAAC 
TCCATGTCTG ATGATCTGGA CAACCTCAAG AAGATGGGGC AAGATCTGGC TCAGGTCCTG 
AGGCAGCTCA CCAGTGACTA CACAATAGGA TTTGGCAAGT TCGTGGACAA AGTCAGCGTC 
CCTCAGACAG ACATGAGGCC TGAGAAGCTG AAGGAACCCT GGCCCAACAG CGACCCCCCC 
TTCTCCTTCA AGAATGTCAT CAGCCTGACG GAAGATGTGG AGGAGTTCCG GAATAAGCTG 
AAGGGGGAGC GCATCTCAGG CAACCTGGAT GCCCCGGAAG GAGGTTTTGA TGCCATCCTG 
CAGACGGCCG TGTGCACCAG TGACATTGGC TGGCGCCCTG ACAGCACCCA CTTGCTGGTG 
TTCTCCACCG AGTCAGCCTT CCACTACGAG GCCGACGGGG CCAACGTGCT GGCCGGCATC 
ATGAGGCGTA ACGACGAGGA ATGCCACCTG GACCCCACGG GCACCTACAC CCAGTACAAG 
ATGCAGGACT ACCCGTCGGT GCCCACCCTG GTGCGCCTGC TCGGCCAACA CAACATCATC 
CCCATCTTCG CCGTCACCAA CTACTCCTAT AGCTACTATG AGAGGCTGTC GTCATATTTC 
CCCGTCTCTT CACTGGGGGT GCTGCAGGAG GACTCGTCCA ACATTGTGGA TCTGCTGCAG 
GAAGCCTTCA ACCGCATCCG CTCCAACCTG GACATTCGGG CCCTGGACAG TCCTCGAGGC 
CTGCGGACAG AGGTCACCTC TAGGATGTTC CAGAAGACGG ACACGGGGTC CTTTTTCATC 
CGGCGAGGGG AAGTGGGCAC ATACCAAGTG CAGCTCCGGG CCATCGAGGA CCTGGACGGG 
GCTCATGTGT GTCAGCTTCT GGAGGCAGAC CAGAAGGGCA CCATCCATCT GAAGCCATCC 
TTCTCCAACG GCCTCCGGAT GGACGTGGGC GTCATCTGTG ACGTGTGCTC CTGTGAGCTG 
CAAAAAGAGG AGCGGTCATC TCGCTGCAAC TTCCACGGGG ACTTCATGTG CGGACACTGT 
GTGTGCCACG AGGGCTGGAG CGGCAAGACC TGTAACTGCT CCACCGGCTC CCAGAGCGAC 
CTGCAGCCCT GCCGGCGGGA GGGCGAGGAC AAGGTGTGCT CCGGGCGGGG CGAGTGCCAG 
TGTGGCCACT GTGTGTGCTA TGGCGAAGGC CGCTACGAGG GTCAGTTCTG CGAATACGAC 
AACTTCCAGT GTCCCCGCAC CTCCGGGTTC CTCTGCAATG ACCGGGGACG CTGCTCCATG 
GGTCAGTGCG TGTGTGAGCC TGGCTGGACA GGCCTGAGCT GTGACTGTCC TCTCAGCAAT 
GCCACCTGCA TCGACAGTAA CGGGGGTCTC TGTAATGGGC GTGGCCACTG CGAGTGCGGC 
CGCTGCCACT GCAACCAACA GTCGCTCTAC ACGGACACCG TCTGCGAGAT CAACTACTCG 
GCGATCCACC TGGGCCTCTG TGAGGACCTG CGCTCCTGCG TGCAGTGCCA GGCCTGGGGC 
ACCGGTGAAA AGAAGGGGCG CACGTGCGAG GAGTGCAGCT TCAAGGTCAA GATGGTGGAT 
GAGCTCAAGA AAGCGGAGGA GGTGGTGGAG CACTGCTCCT TCCGGGACGA GGACGACGAC 
TGTACCTACA GTTACACCGT GGAGGGGGAC AGCGCCCCCG GGCCCAACAG CACCGTCTTG 
GTGCAGAGGA GGAAGGAATG CCCCCCGGGC ACCTTCTGGT GGCTCATCCC CCTGCTCATC 
TTCCTCCTCC TGTTCCCGGT CCTGCTGCTG CTGCTCTGCT GGAAGTACTG TGCCTGCTGC 
AAGGCCTGCC TGGCCCTTCT CCCTTGCTGC AACCGAGGTC ACATGGTGGG CTTCAAGGAA 
GACCATTACA TGCTGCGAGA GAACCTGATG GCCTCGGACC ACTTGGACAC GCCCTTGCTG 
CGCAGCGGGA ACCTCAAGGG TCGCGACACA GTCCGATGGA AGATCACCAA CAACGTGCAG 
CGGCCGGGCT TCGCCTCGCA CGCTGCAGGC ATCAACCCCT CAGAGCTGGT GCCCTACGGA 
CTGTCCCTGC GGCTTGCTCG CCTCTGCACC GAGAACCTGC TGAAGCCTGG CACGCGAGAA 
TGCGACCAGC TGCGCCAGGA GGTGGAGGAG AATCTGAACG AGGTGTACCG ACAAATCACA 
GGCGCTCACA ACCTCCAGCA GACCAAGTTC CGGCAGCAGC CCAACGCCGG GAAAAAGCAG 
GACCACACCA TTGTGGACAC TGTGCTGATG GCTCCCCGCT CGGCCAAGCA GGCCCTGCTG 
AAGCTGACGG AGAAGCACGT GGAGCAGGGG GCCTTCCATG AGCTCAAGGT GGCCCCCGGC 
TATTACACGC TCACCGCAGA CCAGGATGCC CGGGGCATGG TGGAATTCCA GGAGGGCGTG 
GAGCTGGTGG ACGTGCGGGT GCCTTTGTTT ATCCGGCCCG AGGACGACGA CGAGAAGCAG 
CTGCTGGTGG AGGCCATCGA CGTGCCTGTG GGCACCGCCA CCCTCGGACG TCGCCTGGTC 
AATATCACCA TCATCAAGGA GCAAGCCAGC GGGATCGTGT CCTTTGAGCA GCCCGAGTAC 
TTGGTCAGCA GCGGGGAGCA CGTTGCCCGC ATCCCCGTGG TCCGGCGCAT CCTGGACAGC 
GGCAAATCAC AGGTCTCCTA CCGCACACAG GATAACACCG CCAAGGCCAA CCGGGACTAC 
ATCCCCATGG AGGGTGAGCT GCTGTTCCAA CCTGGGGAGA CCTGGAAGGA GCTGCAGGTG 
AAGCTCCTGG AGCTGCAGGA GATGGACTCC CTTCTGCGGG GCCCCCAGAC CCGCCGCTTC 
TACATCCAAC TCAGCAACCC CAAGTTCGGG GCCCGCCTGG GCCAGCCCCA GTGTGCCACT 
GTCATCACTG GGGACCGAGA CGAGCTGGAC CGGAATGTAA TGAACCAGAC CGTGTCATCA 
CCACCACCGC CCCGGGGTGA CCTGGGTGCC CCACAGAACC CCAATGCCAA GGCTGCTGGG 
TCCCGGAAGA TCCACTTCAA CTGGCTGCCT CCTCCTGGCA AGCCGACAGG GTACCGGGTG 
AAATATTGGA TCCAGGGTGA CTCCGAGTCT GAAGCCCACC TGCTTGACAG CAAGGTGCCC 
TCAGTGGAGC TCACCAACCT GTACCCATAT TGTGACTACG AGATGAAGGT GTGCGCCTAT 
GGGGCGCAGG GCGAGGGCCC CTACAGCTCC CCGGTGTCCT GTCGCACCCA CCAGGAAGTG 
CCCAGCGAGC CCGGGCGTCT GGCCTTCAAC GTCGTCTCCT CCACCGTGAC CCAGCTGAGC 
TGGGCCGAGC CGGCTGAGAC CAACGGCGAG ATCACAGCCT ACGAGGTCTG CTATGGCCTG 
GTCAATGAGG ACAACCGACC CATCGGGCCC ATGAAGAAGG TGCTGGTGGA CAGCCCCAAG 
AAGCGGACGC TGCTCATCGA GAACCTGCGC GAGTCCCAGC CATACCGCTA CACGGTCAAG 
GCTCGCAACG GGGCAGGCTG GGGGCCTGAG CGGGAGGCCA TCATCAACCT GGCCACCCAG 
CCCAAGCGAC CCATGTCCAT CCCCATCATC CCTGACATCC CCATCGTGGA CGCCCAGAGT 
GGGGAGGACT ATGAGAGCTT CCTCATGTAC AGCGATGATG TGCTCCGCTC TCCAGCCGGC 
AGCCAGAGGC CCAGCGTCTC TGATGACACC GAGCACCTGG TGAACGGGCG GATGGACTTT 
GCCTTCCCGG GCAGCGCCAA CTCCCTGCAC AGGATGACCG TGAGCACCGC GGCCCACGGC 
GCCCACCTGA GCCCGCAGTT GTCCCACCGC GTGCTGAGCA CCTCGTCCAC CCTCACGCGG 
GACTACCACT CGCTGACCCG CACGGAGCAC TCGCACTCCG CCACGCTGCC CAGGGACTAC 
TCCACTCTCA CCTCCCTTTC CTCCCAAAGC CTCCCTCCCA TCTGGGAACA CGGGAGGAGC 
AGGCTTCCGC TGTCCTGGGC CCTAGGGTCC CGGAGTCGGG CTCAGATGAA GGGAGCCCCC 
CGCTCCGGGG ACTTGAGAGA CTCTATAATC CTGGCTGGGC AGCCAGCAGC GCCCTCTTGG 
GGCCCAGGCT CCCGCTTGGC TGCGGGTGTG CCCAACACAC CCACCCGCCT GGTGTTCTCC 
GCCCTTGGAC CCACGTCTCT GAAAGTGAGC TGGCAGGAGC CGCAGTGTGA GCGTGCGCTG 
CAGGGCTACA GTGTGGAGTA CCAGCTGCTG AACGGCGGTG AGCTGTATCG CCTCAACATC 
CCCAACCCCA GCCAGACCTC GGTGGTGGTG GAGGACCTCC TGCCCAACCA CTCCTACGTG 
TTCCGTGTGC GGGCCCAGAG CCAGGAGGGC TGGGGCCCGG AGCGTGAGGG TGTCATCACC 
ATTGAGTCAC AGGTGCACCC GCAGAGCCCG CTCTGCCCCC TGCCGGGCTC CGCCTTCACT 
TTGAGCACAC CCAGCGCCCC GGGCCCGCTG GTGTTCACTG CCCTGAGCCC CGACTCGCTG 
CAGCTGAGCT GGGAGCGGCC CCGCAGGCCA GATGGCGACA TTCTGGGCTA CCTGGTGACG 
TGTGAGATGG CCCACGGAGG AGAGCCAGCC ACTACGTTCC TGGTTGACGG CGACAGCCCT 
GAGAGCCGGC TGACCGTGCC TGGCCTCAGC GAGAACGTGC CCTACAAGTT CAAGGTGCAG 
GCCAAGACCA CCCAAGGCTT CGGGCCGGAG CGAGAGGGAA TCATCACCAT CGAGTCCCAG 
GATGGAGGAC CCTTTCCACA GCTGGGCGGC CACTTGGGGC TCTTCCAGCA CCCAGCGTCA 
GGGGAATACA GCAGCATCAC CACCCACTCC AGCACCACTG AACCCTTCCT ACTGGATGGA 
CTGACCCTGG GCTCCCAGCA TCTGGAAGCA AGTGGCTCCC TCACCCGGCA TGTGACACAG 
GAGTTTGTGA GCCGGACTCT GACCACCAGT GGGACCCTCA GCACCCACGT GGACCAACAG 
TTCTTCCAGA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835565.1
CDS	229..5643
Translation	MAGPHPSPWTRLLLAALLSVSFPGHMANRCKKAQVKSCTECIRVDKDCAYCTDEVFKERRCNTQAELLAAGCRLESVVVMESSFEITEERQIDTTLRRSQVSPQALRVRLRPGEERHFELQVFEPLESPMDLYILMDFSNSMSDDLDNLKKMGQDLAQVLRQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTEDVEEFRNKLKGERISGNLDAPEGGFDAILQTAVCTSDIGWRPDSTHLLVFSTESAFHYEADGANVLAGIMRRNDEECHLDPTGTYTQYKMQDYPSVPTLVRLLGQHNIIPIFAVTNYSYSYYERLSSYFPVSSLGVLQEDSSNIVDLLQEAFNRIRSNLDIRALDSPRGLRTEVTSRMFQKTDTGSFFIRRGEVGTYQVQLRAIEDLDGAHVCQLLEADQKGTIHLKPSFSNGLRMDVGVICDVCSCELQKEERSSRCNFHGDFMCGHCVCHEGWSGKTCNCSTGSQSDLQPCRREGEDKVCSGRGECQCGHCVCYGEGRYEGQFCEYDNFQCPRTSGFLCNDRGRCSMGQCVCEPGWTGLSCDCPLSNATCIDSNGGLCNGRGHCECGRCHCNQQSLYTDTVCEINYSAIHLGLCEDLRSCVQCQAWGTGEKKGRTCEECSFKVKMVDELKKAEEVVEHCSFRDEDDDCTYSYTVEGDSAPGPNSTVLVQRRKECPPGTFWWLIPLLIFLLLFPVLLLLLCWKYCACCKACLALLPCCNRGHMVGFKEDHYMLRENLMASDHLDTPLLRSGNLKGRDTVRWKITNNVQRPGFASHAAGINPSELVPYGLSLRLARLCTENLLKPGTRECDQLRQEVEENLNEVYRQITGAHNLQQTKFRQQPNAGKKQDHTIVDTVLMAPRSAKQALLKLTEKHVEQGAFHELKVAPGYYTLTADQDARGMVEFQEGVELVDVRVPLFIRPEDDDEKQLLVEAIDVPVGTATLGRRLVNITIIKEQASGIVSFEQPEYLVSSGEHVARIPVVRRILDSGKSQVSYRTQDNTAKANRDYIPMEGELLFQPGETWKELQVKLLELQEMDSLLRGPQTRRFYIQLSNPKFGARLGQPQCATVITGDRDELDRNVMNQTVSSPPPPRGDLGAPQNPNAKAAGSRKIHFNWLPPPGKPTGYRVKYWIQGDSESEAHLLDSKVPSVELTNLYPYCDYEMKVCAYGAQGEGPYSSPVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYGLVNEDNRPIGPMKKVLVDSPKKRTLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDIPIVDAQSGEDYESFLMYSDDVLRSPAGSQRPSVSDDTEHLVNGRMDFAFPGSANSLHRMTVSTAAHGAHLSPQLSHRVLSTSSTLTRDYHSLTRTEHSHSATLPRDYSTLTSLSSQSLPPIWEHGRSRLPLSWALGSRSRAQMKGAPRSGDLRDSIILAGQPAAPSWGPGSRLAAGVPNTPTRLVFSALGPTSLKVSWQEPQCERALQGYSVEYQLLNGGELYRLNIPNPSQTSVVVEDLLPNHSYVFRVRAQSQEGWGPEREGVITIESQVHPQSPLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPDGDILGYLVTCEMAHGGEPATTFLVDGDSPESRLTVPGLSENVPYKFKVQAKTTQGFGPEREGIITIESQDGGPFPQLGGHLGLFQHPASGEYSSITTHSSTTEPFLLDGLTLGSQHLEASGSLTRHVTQEFVSRTLTTSGTLSTHVDQQFFQT

Target ORF information:

RefSeq Version	XM_024979797.1
Organism	Bos taurus(cattle)
Definition	Bos taurus integrin subunit beta 4 (ITGB4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979797.1

ORF Insert Sequence:

ATGGCAGGGC CACATCCCAG CCCGTGGACC AGGCTGCTGC TGGCAGCGCT GCTGAGCGTC 
AGCTTCCCCG GGCACATGGC GAACCGCTGC AAGAAGGCCC AGGTGAAGAG CTGTACCGAG 
TGCATCCGCG TGGACAAGGA CTGCGCCTAC TGCACGGACG AGGTGTTCAA GGAACGGCGC 
TGTAACACCC AGGCCGAGCT GCTGGCTGCC GGCTGCCGGC TGGAGAGCGT GGTGGTCATG 
GAGAGCAGCT TCGAGATCAC GGAGGAAAGG CAGATCGACA CCACGCTGCG CCGCAGCCAG 
GTGTCCCCCC AGGCGCTGCG GGTGCGGCTG CGGCCGGGCG AGGAGCGGCA CTTCGAGCTG 
CAGGTGTTCG AGCCCCTGGA GAGCCCCATG GACCTGTATA TCCTCATGGA CTTCTCCAAC 
TCCATGTCTG ATGATCTGGA CAACCTCAAG AAGATGGGGC AAGATCTGGC TCAGGTCCTG 
AGGCAGCTCA CCAGTGACTA CACAATAGGA TTTGGCAAGT TCGTGGACAA AGTCAGCGTC 
CCTCAGACAG ACATGAGGCC TGAGAAGCTG AAGGAACCCT GGCCCAACAG CGACCCCCCC 
TTCTCCTTCA AGAATGTCAT CAGCCTGACG GAAGATGTGG AGGAGTTCCG GAATAAGCTG 
AAGGGGGAGC GCATCTCAGG CAACCTGGAT GCCCCGGAAG GAGGTTTTGA TGCCATCCTG 
CAGACGGCCG TGTGCACCAG TGACATTGGC TGGCGCCCTG ACAGCACCCA CTTGCTGGTG 
TTCTCCACCG AGTCAGCCTT CCACTACGAG GCCGACGGGG CCAACGTGCT GGCCGGCATC 
ATGAGGCGTA ACGACGAGGA ATGCCACCTG GACCCCACGG GCACCTACAC CCAGTACAAG 
ATGCAGGACT ACCCGTCGGT GCCCACCCTG GTGCGCCTGC TCGGCCAACA CAACATCATC 
CCCATCTTCG CCGTCACCAA CTACTCCTAT AGCTACTATG AGAGGCTGTC GTCATATTTC 
CCCGTCTCTT CACTGGGGGT GCTGCAGGAG GACTCGTCCA ACATTGTGGA TCTGCTGCAG 
GAAGCCTTCA ACCGCATCCG CTCCAACCTG GACATTCGGG CCCTGGACAG TCCTCGAGGC 
CTGCGGACAG AGGTCACCTC TAGGATGTTC CAGAAGACGG ACACGGGGTC CTTTTTCATC 
CGGCGAGGGG AAGTGGGCAC ATACCAAGTG CAGCTCCGGG CCATCGAGGA CCTGGACGGG 
GCTCATGTGT GTCAGCTTCT GGAGGCAGAC CAGAAGGGCA CCATCCATCT GAAGCCATCC 
TTCTCCAACG GCCTCCGGAT GGACGTGGGC GTCATCTGTG ACGTGTGCTC CTGTGAGCTG 
CAAAAAGAGG AGCGGTCATC TCGCTGCAAC TTCCACGGGG ACTTCATGTG CGGACACTGT 
GTGTGCCACG AGGGCTGGAG CGGCAAGACC TGTAACTGCT CCACCGGCTC CCAGAGCGAC 
CTGCAGCCCT GCCGGCGGGA GGGCGAGGAC AAGGTGTGCT CCGGGCGGGG CGAGTGCCAG 
TGTGGCCACT GTGTGTGCTA TGGCGAAGGC CGCTACGAGG GTCAGTTCTG CGAATACGAC 
AACTTCCAGT GTCCCCGCAC CTCCGGGTTC CTCTGCAATG ACCGGGGACG CTGCTCCATG 
GGTCAGTGCG TGTGTGAGCC TGGCTGGACA GGCCTGAGCT GTGACTGTCC TCTCAGCAAT 
GCCACCTGCA TCGACAGTAA CGGGGGTCTC TGTAATGGGC GTGGCCACTG CGAGTGCGGC 
CGCTGCCACT GCAACCAACA GTCGCTCTAC ACGGACACCG TCTGCGAGAT CAACTACTCG 
GCGATCCACC TGGGCCTCTG TGAGGACCTG CGCTCCTGCG TGCAGTGCCA GGCCTGGGGC 
ACCGGTGAAA AGAAGGGGCG CACGTGCGAG GAGTGCAGCT TCAAGGTCAA GATGGTGGAT 
GAGCTCAAGA AAGCGGAGGA GGTGGTGGAG CACTGCTCCT TCCGGGACGA GGACGACGAC 
TGTACCTACA GTTACACCGT GGAGGGGGAC AGCGCCCCCG GGCCCAACAG CACCGTCTTG 
GTGCAGAGGA GGAAGGAATG CCCCCCGGGC ACCTTCTGGT GGCTCATCCC CCTGCTCATC 
TTCCTCCTCC TGTTCCCGGT CCTGCTGCTG CTGCTCTGCT GGAAGTACTG TGCCTGCTGC 
AAGGCCTGCC TGGCCCTTCT CCCTTGCTGC AACCGAGGTC ACATGGTGGG CTTCAAGGAA 
GACCATTACA TGCTGCGAGA GAACCTGATG GCCTCGGACC ACTTGGACAC GCCCTTGCTG 
CGCAGCGGGA ACCTCAAGGG TCGCGACACA GTCCGATGGA AGATCACCAA CAACGTGCAG 
CGGCCGGGCT TCGCCTCGCA CGCTGCAGGC ATCAACCCCT CAGAGCTGGT GCCCTACGGA 
CTGTCCCTGC GGCTTGCTCG CCTCTGCACC GAGAACCTGC TGAAGCCTGG CACGCGAGAA 
TGCGACCAGC TGCGCCAGGA GGTGGAGGAG AATCTGAACG AGGTGTACCG ACAAATCACA 
GGCGCTCACA ACCTCCAGCA GACCAAGTTC CGGCAGCAGC CCAACGCCGG GAAAAAGCAG 
GACCACACCA TTGTGGACAC TGTGCTGATG GCTCCCCGCT CGGCCAAGCA GGCCCTGCTG 
AAGCTGACGG AGAAGCACGT GGAGCAGGGG GCCTTCCATG AGCTCAAGGT GGCCCCCGGC 
TATTACACGC TCACCGCAGA CCAGGATGCC CGGGGCATGG TGGAATTCCA GGAGGGCGTG 
GAGCTGGTGG ACGTGCGGGT GCCTTTGTTT ATCCGGCCCG AGGACGACGA CGAGAAGCAG 
CTGCTGGTGG AGGCCATCGA CGTGCCTGTG GGCACCGCCA CCCTCGGACG TCGCCTGGTC 
AATATCACCA TCATCAAGGA GCAAGCCAGC GGGATCGTGT CCTTTGAGCA GCCCGAGTAC 
TTGGTCAGCA GCGGGGAGCA CGTTGCCCGC ATCCCCGTGG TCCGGCGCAT CCTGGACAGC 
GGCAAATCAC AGGTCTCCTA CCGCACACAG GATAACACCG CCAAGGCCAA CCGGGACTAC 
ATCCCCATGG AGGGTGAGCT GCTGTTCCAA CCTGGGGAGA CCTGGAAGGA GCTGCAGGTG 
AAGCTCCTGG AGCTGCAGGA GATGGACTCC CTTCTGCGGG GCCCCCAGAC CCGCCGCTTC 
TACATCCAAC TCAGCAACCC CAAGTTCGGG GCCCGCCTGG GCCAGCCCCA GTGTGCCACT 
GTCATCACTG GGGACCGAGA CGAGCTGGAC CGGAATGTAA TGAACCAGAC CGTGTCATCA 
CCACCACCGC CCCGGGGTGA CCTGGGTGCC CCACAGAACC CCAATGCCAA GGCTGCTGGG 
TCCCGGAAGA TCCACTTCAA CTGGCTGCCT CCTCCTGGCA AGCCGACAGG GTACCGGGTG 
AAATATTGGA TCCAGGGTGA CTCCGAGTCT GAAGCCCACC TGCTTGACAG CAAGGTGCCC 
TCAGTGGAGC TCACCAACCT GTACCCATAT TGTGACTACG AGATGAAGGT GTGCGCCTAT 
GGGGCGCAGG GCGAGGGCCC CTACAGCTCC CCGGTGTCCT GTCGCACCCA CCAGGAAGTG 
CCCAGCGAGC CCGGGCGTCT GGCCTTCAAC GTCGTCTCCT CCACCGTGAC CCAGCTGAGC 
TGGGCCGAGC CGGCTGAGAC CAACGGCGAG ATCACAGCCT ACGAGGTCTG CTATGGCCTG 
GTCAATGAGG ACAACCGACC CATCGGGCCC ATGAAGAAGG TGCTGGTGGA CAGCCCCAAG 
AAGCGGACGC TGCTCATCGA GAACCTGCGC GAGTCCCAGC CATACCGCTA CACGGTCAAG 
GCTCGCAACG GGGCAGGCTG GGGGCCTGAG CGGGAGGCCA TCATCAACCT GGCCACCCAG 
CCCAAGCGAC CCATGTCCAT CCCCATCATC CCTGACATCC CCATCGTGGA CGCCCAGAGT 
GGGGAGGACT ATGAGAGCTT CCTCATGTAC AGCGATGATG TGCTCCGCTC TCCAGCCGGC 
AGCCAGAGGC CCAGCGTCTC TGATGACACC GAGCACCTGG TGAACGGGCG GATGGACTTT 
GCCTTCCCGG GCAGCGCCAA CTCCCTGCAC AGGATGACCG TGAGCACCGC GGCCCACGGC 
GCCCACCTGA GCCCGCAGTT GTCCCACCGC GTGCTGAGCA CCTCGTCCAC CCTCACGCGG 
GACTACCACT CGCTGACCCG CACGGAGCAC TCGCACTCCG CCACGCTGCC CAGGGACTAC 
TCCACTCTCA CCTCCCTTTC CTCCCAAAGC CTCCCTCCCA TCTGGGAACA CGGGAGGAGC 
AGGCTTCCGC TGTCCTGGGC CCTAGGGTCC CGGAGTCGGG CTCAGATGAA GGGAGCCCCC 
CGCTCCGGGG ACTTGAGAGA CTCTATAATC CTGGCTGGGC AGCCAGCAGC GCCCTCTTGG 
GGCCCAGGCT CCCGCTTGGC TGCGGGTGTG CCCAACACAC CCACCCGCCT GGTGTTCTCC 
GCCCTTGGAC CCACGTCTCT GAAAGTGAGC TGGCAGGAGC CGCAGTGTGA GCGTGCGCTG 
CAGGGCTACA GTGTGGAGTA CCAGCTGCTG AACGGCGGTG AGCTGTATCG CCTCAACATC 
CCCAACCCCA GCCAGACCTC GGTGGTGGTG GAGGACCTCC TGCCCAACCA CTCCTACGTG 
TTCCGTGTGC GGGCCCAGAG CCAGGAGGGC TGGGGCCCGG AGCGTGAGGG TGTCATCACC 
ATTGAGTCAC AGGTGCACCC GCAGAGCCCG CTCTGCCCCC TGCCGGGCTC CGCCTTCACT 
TTGAGCACAC CCAGCGCCCC GGGCCCGCTG GTGTTCACTG CCCTGAGCCC CGACTCGCTG 
CAGCTGAGCT GGGAGCGGCC CCGCAGGCCA GATGGCGACA TTCTGGGCTA CCTGGTGACG 
TGTGAGATGG CCCACGGAGG AGAGCCAGCC ACTACGTTCC TGGTTGACGG CGACAGCCCT 
GAGAGCCGGC TGACCGTGCC TGGCCTCAGC GAGAACGTGC CCTACAAGTT CAAGGTGCAG 
GCCAAGACCA CCCAAGGCTT CGGGCCGGAG CGAGAGGGAA TCATCACCAT CGAGTCCCAG 
GATGGAGGAC CCTTTCCACA GCTGGGCGGC CACTTGGGGC TCTTCCAGCA CCCAGCGTCA 
GGGGAATACA GCAGCATCAC CACCCACTCC AGCACCACTG AACCCTTCCT ACTGGATGGA 
CTGACCCTGG GCTCCCAGCA TCTGGAAGCA AGTGGCTCCC TCACCCGGCA TGTGACACAG 
GAGTTTGTGA GCCGGACTCT GACCACCAGT GGGACCCTCA GCACCCACGT GGACCAACAG 
TTCTTCCAGA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITGB4 ( XM_024979798.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835566.1 Bos taurus integrin subunit beta 4 (ITGB4), transcript variant X4, mRNA.

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CloneID	OBa226112
Clone ID Related Accession (Same CDS sequence)	XM_024979798.1
Accession Version	XM_024979798.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	integrin beta-4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGC CACATCCCAG CCCGTGGACC AGGCTGCTGC TGGCAGCGCT GCTGAGCGTC 
AGCTTCCCCG GGCACATGGC GAACCGCTGC AAGAAGGCCC AGGTGAAGAG CTGTACCGAG 
TGCATCCGCG TGGACAAGGA CTGCGCCTAC TGCACGGACG AGGTGTTCAA GGAACGGCGC 
TGTAACACCC AGGCCGAGCT GCTGGCTGCC GGCTGCCGGC TGGAGAGCGT GGTGGTCATG 
GAGAGCAGCT TCGAGATCAC GGAGGAAAGG CAGATCGACA CCACGCTGCG CCGCAGCCAG 
GTGTCCCCCC AGGCGCTGCG GGTGCGGCTG CGGCCGGGCG AGGAGCGGCA CTTCGAGCTG 
CAGGTGTTCG AGCCCCTGGA GAGCCCCATG GACCTGTATA TCCTCATGGA CTTCTCCAAC 
TCCATGTCTG ATGATCTGGA CAACCTCAAG AAGATGGGGC AAGATCTGGC TCAGGTCCTG 
AGGCAGCTCA CCAGTGACTA CACAATAGGA TTTGGCAAGT TCGTGGACAA AGTCAGCGTC 
CCTCAGACAG ACATGAGGCC TGAGAAGCTG AAGGAACCCT GGCCCAACAG CGACCCCCCC 
TTCTCCTTCA AGAATGTCAT CAGCCTGACG GAAGATGTGG AGGAGTTCCG GAATAAGCTG 
AAGGGGGAGC GCATCTCAGG CAACCTGGAT GCCCCGGAAG GAGGTTTTGA TGCCATCCTG 
CAGACGGCCG TGTGCACCAG TGACATTGGC TGGCGCCCTG ACAGCACCCA CTTGCTGGTG 
TTCTCCACCG AGTCAGCCTT CCACTACGAG GCCGACGGGG CCAACGTGCT GGCCGGCATC 
ATGAGGCGTA ACGACGAGGA ATGCCACCTG GACCCCACGG GCACCTACAC CCAGTACAAG 
ATGCAGGACT ACCCGTCGGT GCCCACCCTG GTGCGCCTGC TCGGCCAACA CAACATCATC 
CCCATCTTCG CCGTCACCAA CTACTCCTAT AGCTACTATG AGAGGCTGTC GTCATATTTC 
CCCGTCTCTT CACTGGGGGT GCTGCAGGAG GACTCGTCCA ACATTGTGGA TCTGCTGCAG 
GAAGCCTTCA ACCGCATCCG CTCCAACCTG GACATTCGGG CCCTGGACAG TCCTCGAGGC 
CTGCGGACAG AGGTCACCTC TAGGATGTTC CAGAAGACGG ACACGGGGTC CTTTTTCATC 
CGGCGAGGGG AAGTGGGCAC ATACCAAGTG CAGCTCCGGG CCATCGAGGA CCTGGACGGG 
GCTCATGTGT GTCAGCTTCT GGAGGCAGAC CAGAAGGGCA CCATCCATCT GAAGCCATCC 
TTCTCCAACG GCCTCCGGAT GGACGTGGGC GTCATCTGTG ACGTGTGCTC CTGTGAGCTG 
CAAAAAGAGG AGCGGTCATC TCGCTGCAAC TTCCACGGGG ACTTCATGTG CGGACACTGT 
GTGTGCCACG AGGGCTGGAG CGGCAAGACC TGTAACTGCT CCACCGGCTC CCAGAGCGAC 
CTGCAGCCCT GCCGGCGGGA GGGCGAGGAC AAGGTGTGCT CCGGGCGGGG CGAGTGCCAG 
TGTGGCCACT GTGTGTGCTA TGGCGAAGGC CGCTACGAGG GTCAGTTCTG CGAATACGAC 
AACTTCCAGT GTCCCCGCAC CTCCGGGTTC CTCTGCAATG ACCGGGGACG CTGCTCCATG 
GGTCAGTGCG TGTGTGAGCC TGGCTGGACA GGCCTGAGCT GTGACTGTCC TCTCAGCAAT 
GCCACCTGCA TCGACAGTAA CGGGGGTCTC TGTAATGGGC GTGGCCACTG CGAGTGCGGC 
CGCTGCCACT GCAACCAACA GTCGCTCTAC ACGGACACCG TCTGCGAGAT CAACTACTCG 
GCGATCCACC TGGGCCTCTG TGAGGACCTG CGCTCCTGCG TGCAGTGCCA GGCCTGGGGC 
ACCGGTGAAA AGAAGGGGCG CACGTGCGAG GAGTGCAGCT TCAAGGTCAA GATGGTGGAT 
GAGCTCAAGA AAGCGGAGGA GGTGGTGGAG CACTGCTCCT TCCGGGACGA GGACGACGAC 
TGTACCTACA GTTACACCGT GGAGGGGGAC AGCGCCCCCG GGCCCAACAG CACCGTCTTG 
GTGCAGAGGA GGAAGGAATG CCCCCCGGGC ACCTTCTGGT GGCTCATCCC CCTGCTCATC 
TTCCTCCTCC TGTTCCCGGT CCTGCTGCTG CTGCTCTGCT GGAAGTACTG TGCCTGCTGC 
AAGGCCTGCC TGGCCCTTCT CCCTTGCTGC AACCGAGGTC ACATGGTGGG CTTCAAGGAA 
GACCATTACA TGCTGCGAGA GAACCTGATG GCCTCGGACC ACTTGGACAC GCCCTTGCTG 
CGCAGCGGGA ACCTCAAGGG TCGCGACACA GTCCGATGGA AGATCACCAA CAACGTGCAG 
CGGCCGGGCT TCGCCTCGCA CGCTGCAGGC ATCAACCCCT CAGAGCTGGT GCCCTACGGA 
CTGTCCCTGC GGCTTGCTCG CCTCTGCACC GAGAACCTGC TGAAGCCTGG CACGCGAGAA 
TGCGACCAGC TGCGCCAGGA GGTGGAGGAG AATCTGAACG AGGTGTACCG ACAAATCACA 
GGCGCTCACA ACCTCCAGCA GACCAAGTTC CGGCAGCAGC CCAACGCCGG GAAAAAGCAG 
GACCACACCA TTGTGGACAC TGTGCTGATG GCTCCCCGCT CGGCCAAGCA GGCCCTGCTG 
AAGCTGACGG AGAAGCACGT GGAGCAGGGG GCCTTCCATG AGCTCAAGGT GGCCCCCGGC 
TATTACACGC TCACCGCAGA CCAGGATGCC CGGGGCATGG TGGAATTCCA GGAGGGCGTG 
GAGCTGGTGG ACGTGCGGGT GCCTTTGTTT ATCCGGCCCG AGGACGACGA CGAGAAGCAG 
CTGCTGGTGG AGGCCATCGA CGTGCCTGTG GGCACCGCCA CCCTCGGACG TCGCCTGGTC 
AATATCACCA TCATCAAGGA GCAAGCCAGC GGGATCGTGT CCTTTGAGCA GCCCGAGTAC 
TTGGTCAGCA GCGGGGAGCA CGTTGCCCGC ATCCCCGTGG TCCGGCGCAT CCTGGACAGC 
GGCAAATCAC AGGTCTCCTA CCGCACACAG GATAACACCG CCAAGGCCAA CCGGGACTAC 
ATCCCCATGG AGGGTGAGCT GCTGTTCCAA CCTGGGGAGA CCTGGAAGGA GCTGCAGGTG 
AAGCTCCTGG AGCTGCAGGA GATGGACTCC CTTCTGCGGG GCCCCCAGAC CCGCCGCTTC 
TACATCCAAC TCAGCAACCC CAAGTTCGGG GCCCGCCTGG GCCAGCCCCA GTGTGCCACT 
GTCATCACTG GGGACCGAGA CGAGCTGGAC CGGAATGTAA TGAACCAGAC CGTGTCATCA 
CCACCACCGC CCCGGGGTGA CCTGGGTGCC CCACAGAACC CCAATGCCAA GGCTGCTGGG 
TCCCGGAAGA TCCACTTCAA CTGGCTGCCT CCTCCTGGCA AGCCGACAGG GTACCGGGTG 
AAATATTGGA TCCAGGGTGA CTCCGAGTCT GAAGCCCACC TGCTTGACAG CAAGGTGCCC 
TCAGTGGAGC TCACCAACCT GTACCCATAT TGTGACTACG AGATGAAGGT GTGCGCCTAT 
GGGGCGCAGG GCGAGGGCCC CTACAGCTCC CCGGTGTCCT GTCGCACCCA CCAGGAAGTG 
CCCAGCGAGC CCGGGCGTCT GGCCTTCAAC GTCGTCTCCT CCACCGTGAC CCAGCTGAGC 
TGGGCCGAGC CGGCTGAGAC CAACGGCGAG ATCACAGCCT ACGAGGTCTG CTATGGCCTG 
GTCAATGAGG ACAACCGACC CATCGGGCCC ATGAAGAAGG TGCTGGTGGA CAGCCCCAAG 
AAGCGGACGC TGCTCATCGA GAACCTGCGC GAGTCCCAGC CATACCGCTA CACGGTCAAG 
GCTCGCAACG GGGCAGGCTG GGGGCCTGAG CGGGAGGCCA TCATCAACCT GGCCACCCAG 
CCCAAGCGAC CCATGTCCAT CCCCATCATC CCTGACATCC CCATCGTGGA CGCCCAGAGT 
GGGGAGGACT ATGAGAGCTT CCTCATGTAC AGCGATGATG TGCTCCGCTC TCCAGCCGGC 
AGCCAGAGGC CCAGCGTCTC TGATGACACC GAGCACCTGG TGAACGGGCG GATGGACTTT 
GCCTTCCCGG GCAGCGCCAA CTCCCTGCAC AGGATGACCG TGAGCACCGC GGCCCACGGC 
GCCCACCTGA GCCCGCAGTT GTCCCACCGC GTGCTGAGCA CCTCGTCCAC CCTCACGCGG 
GACTACCACT CGCTGACCCG CACGGAGCAC TCGCACTCCG CCACGCTGCC CAGGGACTAC 
TCCACTCTCA CCTCCCTTTC CTCCCAAAGC TCCCGCTTGG CTGCGGGTGT GCCCAACACA 
CCCACCCGCC TGGTGTTCTC CGCCCTTGGA CCCACGTCTC TGAAAGTGAG CTGGCAGGAG 
CCGCAGTGTG AGCGTGCGCT GCAGGGCTAC AGTGTGGAGT ACCAGCTGCT GAACGGCGGT 
GAGCTGTATC GCCTCAACAT CCCCAACCCC AGCCAGACCT CGGTGGTGGT GGAGGACCTC 
CTGCCCAACC ACTCCTACGT GTTCCGTGTG CGGGCCCAGA GCCAGGAGGG CTGGGGCCCG 
GAGCGTGAGG GTGTCATCAC CATTGAGTCA CAGGTGCACC CGCAGAGCCC GCTCTGCCCC 
CTGCCGGGCT CCGCCTTCAC TTTGAGCACA CCCAGCGCCC CGGGCCCGCT GGTGTTCACT 
GCCCTGAGCC CCGACTCGCT GCAGCTGAGC TGGGAGCGGC CCCGCAGGCC AGATGGCGAC 
ATTCTGGGCT ACCTGGTGAC GTGTGAGATG GCCCACGGAG GAGAGCCAGC CACTACGTTC 
CTGGTTGACG GCGACAGCCC TGAGAGCCGG CTGACCGTGC CTGGCCTCAG CGAGAACGTG 
CCCTACAAGT TCAAGGTGCA GGCCAAGACC ACCCAAGGCT TCGGGCCGGA GCGAGAGGGA 
ATCATCACCA TCGAGTCCCA GGATGGAGGA CCCTTTCCAC AGCTGGGCGG CCACTTGGGG 
CTCTTCCAGC ACCCAGCGTC AGGGGAATAC AGCAGCATCA CCACCCACTC CAGCACCACT 
GAACCCTTCC TACTGGATGG ACTGACCCTG GGCTCCCAGC ATCTGGAAGC AAGTGGCTCC 
CTCACCCGGC ATGTGACACA GGAGTTTGTG AGCCGGACTC TGACCACCAG TGGGACCCTC 
AGCACCCACG TGGACCAACA GTTCTTCCAG ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835566.1
CDS	241..5496
Translation	MAGPHPSPWTRLLLAALLSVSFPGHMANRCKKAQVKSCTECIRVDKDCAYCTDEVFKERRCNTQAELLAAGCRLESVVVMESSFEITEERQIDTTLRRSQVSPQALRVRLRPGEERHFELQVFEPLESPMDLYILMDFSNSMSDDLDNLKKMGQDLAQVLRQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTEDVEEFRNKLKGERISGNLDAPEGGFDAILQTAVCTSDIGWRPDSTHLLVFSTESAFHYEADGANVLAGIMRRNDEECHLDPTGTYTQYKMQDYPSVPTLVRLLGQHNIIPIFAVTNYSYSYYERLSSYFPVSSLGVLQEDSSNIVDLLQEAFNRIRSNLDIRALDSPRGLRTEVTSRMFQKTDTGSFFIRRGEVGTYQVQLRAIEDLDGAHVCQLLEADQKGTIHLKPSFSNGLRMDVGVICDVCSCELQKEERSSRCNFHGDFMCGHCVCHEGWSGKTCNCSTGSQSDLQPCRREGEDKVCSGRGECQCGHCVCYGEGRYEGQFCEYDNFQCPRTSGFLCNDRGRCSMGQCVCEPGWTGLSCDCPLSNATCIDSNGGLCNGRGHCECGRCHCNQQSLYTDTVCEINYSAIHLGLCEDLRSCVQCQAWGTGEKKGRTCEECSFKVKMVDELKKAEEVVEHCSFRDEDDDCTYSYTVEGDSAPGPNSTVLVQRRKECPPGTFWWLIPLLIFLLLFPVLLLLLCWKYCACCKACLALLPCCNRGHMVGFKEDHYMLRENLMASDHLDTPLLRSGNLKGRDTVRWKITNNVQRPGFASHAAGINPSELVPYGLSLRLARLCTENLLKPGTRECDQLRQEVEENLNEVYRQITGAHNLQQTKFRQQPNAGKKQDHTIVDTVLMAPRSAKQALLKLTEKHVEQGAFHELKVAPGYYTLTADQDARGMVEFQEGVELVDVRVPLFIRPEDDDEKQLLVEAIDVPVGTATLGRRLVNITIIKEQASGIVSFEQPEYLVSSGEHVARIPVVRRILDSGKSQVSYRTQDNTAKANRDYIPMEGELLFQPGETWKELQVKLLELQEMDSLLRGPQTRRFYIQLSNPKFGARLGQPQCATVITGDRDELDRNVMNQTVSSPPPPRGDLGAPQNPNAKAAGSRKIHFNWLPPPGKPTGYRVKYWIQGDSESEAHLLDSKVPSVELTNLYPYCDYEMKVCAYGAQGEGPYSSPVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYGLVNEDNRPIGPMKKVLVDSPKKRTLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDIPIVDAQSGEDYESFLMYSDDVLRSPAGSQRPSVSDDTEHLVNGRMDFAFPGSANSLHRMTVSTAAHGAHLSPQLSHRVLSTSSTLTRDYHSLTRTEHSHSATLPRDYSTLTSLSSQSSRLAAGVPNTPTRLVFSALGPTSLKVSWQEPQCERALQGYSVEYQLLNGGELYRLNIPNPSQTSVVVEDLLPNHSYVFRVRAQSQEGWGPEREGVITIESQVHPQSPLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPDGDILGYLVTCEMAHGGEPATTFLVDGDSPESRLTVPGLSENVPYKFKVQAKTTQGFGPEREGIITIESQDGGPFPQLGGHLGLFQHPASGEYSSITTHSSTTEPFLLDGLTLGSQHLEASGSLTRHVTQEFVSRTLTTSGTLSTHVDQQFFQT

Target ORF information:

RefSeq Version	XM_024979798.1
Organism	Bos taurus(cattle)
Definition	Bos taurus integrin subunit beta 4 (ITGB4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979798.1

ORF Insert Sequence:

ATGGCAGGGC CACATCCCAG CCCGTGGACC AGGCTGCTGC TGGCAGCGCT GCTGAGCGTC 
AGCTTCCCCG GGCACATGGC GAACCGCTGC AAGAAGGCCC AGGTGAAGAG CTGTACCGAG 
TGCATCCGCG TGGACAAGGA CTGCGCCTAC TGCACGGACG AGGTGTTCAA GGAACGGCGC 
TGTAACACCC AGGCCGAGCT GCTGGCTGCC GGCTGCCGGC TGGAGAGCGT GGTGGTCATG 
GAGAGCAGCT TCGAGATCAC GGAGGAAAGG CAGATCGACA CCACGCTGCG CCGCAGCCAG 
GTGTCCCCCC AGGCGCTGCG GGTGCGGCTG CGGCCGGGCG AGGAGCGGCA CTTCGAGCTG 
CAGGTGTTCG AGCCCCTGGA GAGCCCCATG GACCTGTATA TCCTCATGGA CTTCTCCAAC 
TCCATGTCTG ATGATCTGGA CAACCTCAAG AAGATGGGGC AAGATCTGGC TCAGGTCCTG 
AGGCAGCTCA CCAGTGACTA CACAATAGGA TTTGGCAAGT TCGTGGACAA AGTCAGCGTC 
CCTCAGACAG ACATGAGGCC TGAGAAGCTG AAGGAACCCT GGCCCAACAG CGACCCCCCC 
TTCTCCTTCA AGAATGTCAT CAGCCTGACG GAAGATGTGG AGGAGTTCCG GAATAAGCTG 
AAGGGGGAGC GCATCTCAGG CAACCTGGAT GCCCCGGAAG GAGGTTTTGA TGCCATCCTG 
CAGACGGCCG TGTGCACCAG TGACATTGGC TGGCGCCCTG ACAGCACCCA CTTGCTGGTG 
TTCTCCACCG AGTCAGCCTT CCACTACGAG GCCGACGGGG CCAACGTGCT GGCCGGCATC 
ATGAGGCGTA ACGACGAGGA ATGCCACCTG GACCCCACGG GCACCTACAC CCAGTACAAG 
ATGCAGGACT ACCCGTCGGT GCCCACCCTG GTGCGCCTGC TCGGCCAACA CAACATCATC 
CCCATCTTCG CCGTCACCAA CTACTCCTAT AGCTACTATG AGAGGCTGTC GTCATATTTC 
CCCGTCTCTT CACTGGGGGT GCTGCAGGAG GACTCGTCCA ACATTGTGGA TCTGCTGCAG 
GAAGCCTTCA ACCGCATCCG CTCCAACCTG GACATTCGGG CCCTGGACAG TCCTCGAGGC 
CTGCGGACAG AGGTCACCTC TAGGATGTTC CAGAAGACGG ACACGGGGTC CTTTTTCATC 
CGGCGAGGGG AAGTGGGCAC ATACCAAGTG CAGCTCCGGG CCATCGAGGA CCTGGACGGG 
GCTCATGTGT GTCAGCTTCT GGAGGCAGAC CAGAAGGGCA CCATCCATCT GAAGCCATCC 
TTCTCCAACG GCCTCCGGAT GGACGTGGGC GTCATCTGTG ACGTGTGCTC CTGTGAGCTG 
CAAAAAGAGG AGCGGTCATC TCGCTGCAAC TTCCACGGGG ACTTCATGTG CGGACACTGT 
GTGTGCCACG AGGGCTGGAG CGGCAAGACC TGTAACTGCT CCACCGGCTC CCAGAGCGAC 
CTGCAGCCCT GCCGGCGGGA GGGCGAGGAC AAGGTGTGCT CCGGGCGGGG CGAGTGCCAG 
TGTGGCCACT GTGTGTGCTA TGGCGAAGGC CGCTACGAGG GTCAGTTCTG CGAATACGAC 
AACTTCCAGT GTCCCCGCAC CTCCGGGTTC CTCTGCAATG ACCGGGGACG CTGCTCCATG 
GGTCAGTGCG TGTGTGAGCC TGGCTGGACA GGCCTGAGCT GTGACTGTCC TCTCAGCAAT 
GCCACCTGCA TCGACAGTAA CGGGGGTCTC TGTAATGGGC GTGGCCACTG CGAGTGCGGC 
CGCTGCCACT GCAACCAACA GTCGCTCTAC ACGGACACCG TCTGCGAGAT CAACTACTCG 
GCGATCCACC TGGGCCTCTG TGAGGACCTG CGCTCCTGCG TGCAGTGCCA GGCCTGGGGC 
ACCGGTGAAA AGAAGGGGCG CACGTGCGAG GAGTGCAGCT TCAAGGTCAA GATGGTGGAT 
GAGCTCAAGA AAGCGGAGGA GGTGGTGGAG CACTGCTCCT TCCGGGACGA GGACGACGAC 
TGTACCTACA GTTACACCGT GGAGGGGGAC AGCGCCCCCG GGCCCAACAG CACCGTCTTG 
GTGCAGAGGA GGAAGGAATG CCCCCCGGGC ACCTTCTGGT GGCTCATCCC CCTGCTCATC 
TTCCTCCTCC TGTTCCCGGT CCTGCTGCTG CTGCTCTGCT GGAAGTACTG TGCCTGCTGC 
AAGGCCTGCC TGGCCCTTCT CCCTTGCTGC AACCGAGGTC ACATGGTGGG CTTCAAGGAA 
GACCATTACA TGCTGCGAGA GAACCTGATG GCCTCGGACC ACTTGGACAC GCCCTTGCTG 
CGCAGCGGGA ACCTCAAGGG TCGCGACACA GTCCGATGGA AGATCACCAA CAACGTGCAG 
CGGCCGGGCT TCGCCTCGCA CGCTGCAGGC ATCAACCCCT CAGAGCTGGT GCCCTACGGA 
CTGTCCCTGC GGCTTGCTCG CCTCTGCACC GAGAACCTGC TGAAGCCTGG CACGCGAGAA 
TGCGACCAGC TGCGCCAGGA GGTGGAGGAG AATCTGAACG AGGTGTACCG ACAAATCACA 
GGCGCTCACA ACCTCCAGCA GACCAAGTTC CGGCAGCAGC CCAACGCCGG GAAAAAGCAG 
GACCACACCA TTGTGGACAC TGTGCTGATG GCTCCCCGCT CGGCCAAGCA GGCCCTGCTG 
AAGCTGACGG AGAAGCACGT GGAGCAGGGG GCCTTCCATG AGCTCAAGGT GGCCCCCGGC 
TATTACACGC TCACCGCAGA CCAGGATGCC CGGGGCATGG TGGAATTCCA GGAGGGCGTG 
GAGCTGGTGG ACGTGCGGGT GCCTTTGTTT ATCCGGCCCG AGGACGACGA CGAGAAGCAG 
CTGCTGGTGG AGGCCATCGA CGTGCCTGTG GGCACCGCCA CCCTCGGACG TCGCCTGGTC 
AATATCACCA TCATCAAGGA GCAAGCCAGC GGGATCGTGT CCTTTGAGCA GCCCGAGTAC 
TTGGTCAGCA GCGGGGAGCA CGTTGCCCGC ATCCCCGTGG TCCGGCGCAT CCTGGACAGC 
GGCAAATCAC AGGTCTCCTA CCGCACACAG GATAACACCG CCAAGGCCAA CCGGGACTAC 
ATCCCCATGG AGGGTGAGCT GCTGTTCCAA CCTGGGGAGA CCTGGAAGGA GCTGCAGGTG 
AAGCTCCTGG AGCTGCAGGA GATGGACTCC CTTCTGCGGG GCCCCCAGAC CCGCCGCTTC 
TACATCCAAC TCAGCAACCC CAAGTTCGGG GCCCGCCTGG GCCAGCCCCA GTGTGCCACT 
GTCATCACTG GGGACCGAGA CGAGCTGGAC CGGAATGTAA TGAACCAGAC CGTGTCATCA 
CCACCACCGC CCCGGGGTGA CCTGGGTGCC CCACAGAACC CCAATGCCAA GGCTGCTGGG 
TCCCGGAAGA TCCACTTCAA CTGGCTGCCT CCTCCTGGCA AGCCGACAGG GTACCGGGTG 
AAATATTGGA TCCAGGGTGA CTCCGAGTCT GAAGCCCACC TGCTTGACAG CAAGGTGCCC 
TCAGTGGAGC TCACCAACCT GTACCCATAT TGTGACTACG AGATGAAGGT GTGCGCCTAT 
GGGGCGCAGG GCGAGGGCCC CTACAGCTCC CCGGTGTCCT GTCGCACCCA CCAGGAAGTG 
CCCAGCGAGC CCGGGCGTCT GGCCTTCAAC GTCGTCTCCT CCACCGTGAC CCAGCTGAGC 
TGGGCCGAGC CGGCTGAGAC CAACGGCGAG ATCACAGCCT ACGAGGTCTG CTATGGCCTG 
GTCAATGAGG ACAACCGACC CATCGGGCCC ATGAAGAAGG TGCTGGTGGA CAGCCCCAAG 
AAGCGGACGC TGCTCATCGA GAACCTGCGC GAGTCCCAGC CATACCGCTA CACGGTCAAG 
GCTCGCAACG GGGCAGGCTG GGGGCCTGAG CGGGAGGCCA TCATCAACCT GGCCACCCAG 
CCCAAGCGAC CCATGTCCAT CCCCATCATC CCTGACATCC CCATCGTGGA CGCCCAGAGT 
GGGGAGGACT ATGAGAGCTT CCTCATGTAC AGCGATGATG TGCTCCGCTC TCCAGCCGGC 
AGCCAGAGGC CCAGCGTCTC TGATGACACC GAGCACCTGG TGAACGGGCG GATGGACTTT 
GCCTTCCCGG GCAGCGCCAA CTCCCTGCAC AGGATGACCG TGAGCACCGC GGCCCACGGC 
GCCCACCTGA GCCCGCAGTT GTCCCACCGC GTGCTGAGCA CCTCGTCCAC CCTCACGCGG 
GACTACCACT CGCTGACCCG CACGGAGCAC TCGCACTCCG CCACGCTGCC CAGGGACTAC 
TCCACTCTCA CCTCCCTTTC CTCCCAAAGC TCCCGCTTGG CTGCGGGTGT GCCCAACACA 
CCCACCCGCC TGGTGTTCTC CGCCCTTGGA CCCACGTCTC TGAAAGTGAG CTGGCAGGAG 
CCGCAGTGTG AGCGTGCGCT GCAGGGCTAC AGTGTGGAGT ACCAGCTGCT GAACGGCGGT 
GAGCTGTATC GCCTCAACAT CCCCAACCCC AGCCAGACCT CGGTGGTGGT GGAGGACCTC 
CTGCCCAACC ACTCCTACGT GTTCCGTGTG CGGGCCCAGA GCCAGGAGGG CTGGGGCCCG 
GAGCGTGAGG GTGTCATCAC CATTGAGTCA CAGGTGCACC CGCAGAGCCC GCTCTGCCCC 
CTGCCGGGCT CCGCCTTCAC TTTGAGCACA CCCAGCGCCC CGGGCCCGCT GGTGTTCACT 
GCCCTGAGCC CCGACTCGCT GCAGCTGAGC TGGGAGCGGC CCCGCAGGCC AGATGGCGAC 
ATTCTGGGCT ACCTGGTGAC GTGTGAGATG GCCCACGGAG GAGAGCCAGC CACTACGTTC 
CTGGTTGACG GCGACAGCCC TGAGAGCCGG CTGACCGTGC CTGGCCTCAG CGAGAACGTG 
CCCTACAAGT TCAAGGTGCA GGCCAAGACC ACCCAAGGCT TCGGGCCGGA GCGAGAGGGA 
ATCATCACCA TCGAGTCCCA GGATGGAGGA CCCTTTCCAC AGCTGGGCGG CCACTTGGGG 
CTCTTCCAGC ACCCAGCGTC AGGGGAATAC AGCAGCATCA CCACCCACTC CAGCACCACT 
GAACCCTTCC TACTGGATGG ACTGACCCTG GGCTCCCAGC ATCTGGAAGC AAGTGGCTCC 
CTCACCCGGC ATGTGACACA GGAGTTTGTG AGCCGGACTC TGACCACCAG TGGGACCCTC 
AGCACCCACG TGGACCAACA GTTCTTCCAG ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITGB4 ( NM_001193257.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001180186.2 Bos taurus integrin, beta 4 (ITGB4), mRNA.

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CloneID	OBa01779
Clone ID Related Accession (Same CDS sequence)	NM_001193257.2
Accession Version	NM_001193257.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2011-11-13
Organism	Bos taurus(cattle)
Product	integrin beta-4 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAFC03117429.1, EH177871.1, AAFC03129054.1 and AAFC03054511.1. On Apr 14, 2011 this sequence version replaced NM_001193257.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

ATGGCAGGGC CACATCCCAG CCCGTGGACC AGGCTGCTGC TGGCAGCGCT GCTGAGCGTC 
AGCTTCCCCG GGCACATGGC GAACCGCTGC AAGAAGGCCC AGGTGAAGAG CTGTACCGAG 
TGCATCCGCG TGGACAAGGA CTGCGCCTAC TGCACGGACG AGGTGTTCAA GGAACGGCGC 
TGTAACACCC AGGCCGAGCT GCTGGCTGCC GGCTGCCGGC TGGAGAGCGT GGTGGTCATG 
GAGAGCAGCT TCGAGATCAC GGAGGAAAGG CAGATCGACA CCACGCTGCG CCGCAGTCAG 
GTGTCCCCCC AGGCGCTGCG GGTGCGGCTG CGGCCGGGCG AGGAGCGGCA CTTCGAGCTG 
CAGGTGTTCG AGCCCCTGGA GAGCCCCATG GACCTGTATA TCCTCATGGA CTTCTCCAAC 
TCCATGTCTG ATGATCTGGA CAACCTCAAG AAGATGGGGC AAGATCTGGC TCAGGTCCTG 
AGGCAGCTCA CCAGTGACTA CACAATAGGA TTTGGCAAGT TCGTGGACAA AGTCAGCGTC 
CCTCAGACAG ACATGAGGCC TGAGAAGCTG AAGGAACCCT GGCCCAACAG CGACCCCCCC 
TTCTCCTTCA AGAATGTCAT CAGCCTGACG GAAGACGTGG AGGAGTTCCG GAATAAGCTG 
AAGGGGGAGC GCATCTCAGG CAACCTGGAT GCCCCGGAAG GAGGTTTTGA TGCCATCCTG 
CAGACGGCCG TGTGCACCAG TGACATTGGC TGGCGCCCTG ACAGCACCCA CTTGCTGGTG 
TTCTCCACCG AGTCAGCCTT CCACTACGAG GCCGACGGGG CCAACGTGCT GGCCGGCATC 
ATGAGGCGTA ACGACGAGGA ATGCCACCTG GACCCCACGG GCACCTACAC CCAGTACAAG 
ATGCAGGACT ACCCGTCGGT GCCCACCCTG GTGCGCCTGC TCGGCCAACA CAACATCATC 
CCCATCTTCG CCGTCACCAA CTACTCCTAT AGCTACTATG AGAGGCTGTC GTCATATTTC 
CCCGTCTCTT CACTGGGGGT GCTGCAGGAG GACTCGTCCA ACATTGTGGA TCTGCTGCAG 
GAAGCCTTCA ACCGCATCCG CTCCAACCTG GACATTCGGG CCCTGGACAG TCCTCGAGGC 
CTGCGGACAG AGGTCACCTC TAGGATGTTC CAGAAGACGG ACACGGGGTC CTTTTTCATC 
CGGCGAGGGG AAGTGGGCAC ATACCAAGTG CAGCTCCGGG CCATCGAGGA CCTGGACGGG 
GCTCATGTGT GTCAGCTTCT GGAGGCAGAC CAGAAGGGCA CCATCCATCT GAAGCCATCC 
TTCTCCAACG GCCTCCGGAT GGACGTGGGC GTCATCTGTG ACGTGTGCTC CTGTGAGCTG 
CAAAAAGAGG AGCGGTCATC TCGCTGCAAC TTCCACGGGG ACTTCATGTG CGGACACTGT 
GTGTGCCACG AGGGCTGGAG CGGCAAGACC TGTAACTGCT CCACGGGCTC CCAGAGCGAC 
CTGCAGCCCT GCCGGCGGGA GGGCGAGGAC AAGGTGTGCT CCGGGCGGGG CGAGTGCCAG 
TGTGGCCACT GTGTGTGCTA TGGCGAAGGC CGCTACGAGG GTCAGTTCTG CGAATACGAC 
AACTTCCAGT GTCCCCGCAC CTCCGGGTTC CTCTGCAATG ACCGGGGACG CTGCTCCATG 
GGTCAGTGCG TGTGTGAGCC TGGCTGGACA GGCCTGAGCT GTGACTGTCC TCTCAGCAAT 
GCCACCTGCA TCGACAGTAA CGGGGGTCTC TGTAATGGGC GTGGCCACTG CGAGTGCGGC 
CGCTGCCACT GCAACCAACA GTCGCTCTAC ACGGACACCG TCTGCGAGAT CAACTACTCG 
GCGATCCACC TGGGCCTCTG TGAGGACCTG CGCTCCTGCG TGCAGTGCCA GGCCTGGGGC 
ACCGGTGAAA AGAAGGGGCG CACGTGCGAG GAGTGCAGCT TCAAGGTCAA GATGGTGGAT 
GAGCTCAAGA AAGCGGAGGA GGTGGTGGAG CACTGCTCCT TCCGGGACGA GGACGACGAC 
TGTACCTACA GTTACACCGT GGAGGGGGAC AGCGCCCCCG GGCCCAACAG CACCGTCTTG 
GTGCAGAGGA GGAAGGAATG CCCCCCGGGC ACCTTCTGGT GGCTCATCCC CCTGCTCATC 
TTCCTCCTCC TGTTCCCGGT CCTGCTGCTG CTGCTCTGCT GGAAGTACTG TGCCTGCTGC 
AAGGCCTGCC TGGCCCTTCT CCCTTGCTGC AACCGAGGTC ACATGGTGGG CTTCAAGGAA 
GACCATTACA TGCTGCGAGA GAACCTGATG GCCTCGGACC ACTTGGACAC GCCCTTGCTG 
CGCAGCGGGA ACCTCAAGGG TCGCGACACA GTCCGATGGA AGATCACCAA CAACGTGCAG 
CGGCCGGGCT TCGCCTCGCA CGCTGCAGGC ATCAACCCCT CAGAGCTGGT GCCCTACGGA 
CTGTCCCTGC GGCTTGCTCG CCTCTGCACC GAGAACCTGC TGAAGCCTGG CACGCGAGAA 
TGCGACCAGC TGCGCCAGGA GGTGGAGGAG AATCTGAACG AGGTGTACCG ACAAATCACA 
GGCGCTCACA ACCTCCAGCA GACCAAGTTC CGGCAGCAGC CCAACGCCGG GAAAAAGCAG 
GACCACACCA TTGTGGACAC TGTGCTGATG GCTCCCCGCT CGGCCAAGCA GGCCCTGCTG 
AAGCTGACGG AGAAGCACGT GGAGCAGGGG GCCTTCCATG AGCTCAAGGT GGCCCCCGGC 
TATTACACGC TCACCGCAGA CCAGGATGCC CGGGGCATGG TGGAATTCCA GGAGGGCGTG 
GAGCTGGTGG ACGTGCGGGT GCCTTTGTTT ATCCGGCCCG AGGACGACGA CGAGAAGCAG 
CTGCTGGTGG AGGCCATCGA CGTGCCTGTG GGCACCGCCA CCCTCGGACG TCGCCTGGTC 
AATATCACCA TCATCAAGGA GCAAGCCAGC GGGATCGTGT CCTTTGAGCA GCCCGAGTAC 
TTGGTCAGCA GCGGGGAGCA CGTTGCCCGC ATCCCCGTGG TCCGGCGCAT CCTGGACAGC 
GGCAAATCAC AGGTCTCCTA CCGCACACAG GATAACACCG CCAAGGCCAA CCGGGACTAC 
ATCCCCATGG AGGGTGAGCT GCTGTTCCAA CCTGGGGAGA CCTGGAAGGA GCTGCAGGTG 
AAGCTCCTGG AGCTGCAGGA GATGGACTCC CTTCTGCGGG GCCCCCAGAC CCGCCGCTTC 
TACATCCAAC TCAGCAACCC CAAGTTCGGG GCCCGCCTGG GCCAGCCCCA GTGTGCCACT 
GTCATCACTG GGGACCGAGA CGAGCTGGAC CGGAATGTAA TGAACCAGAC CGTGTCATCA 
CCACCACCGC CCCGGGGTGA CCTGGGTGCC CCACAGAACC CCAATGCCAA GGCTGCTGGG 
TCCCGGAAGA TCCACTTCAA CTGGCTGCCT CCTCCTGGCA AGCCGACAGG GTACCGGGTG 
AAATATTGGA TCCAGGGTGA CTCCGAGTCT GAAGCCCACC TGCTTGACAG CAAGGTGCCC 
TCAGTGGAGC TCACCAACCT GTACCCATAT TGTGACTACG AGATGAAGGT GTGCGCCTAT 
GGGGCGCAGG GCGAGGGCCC CTACAGCTCC CCGGTGTCCT GTCGCACCCA CCAGGAAGTG 
CCCAGCGAGC CCGGGCGTCT GGCCTTCAAC GTCGTCTCCT CCACCGTGAC CCAGCTGAGC 
TGGGCCGAGC CGGCTGAGAC CAACGGCGAG ATCACAGCCT ACGAGGTCTG CTATGGCCTG 
GTCAATGAGG ACAACCGACC CATCGGGCCC ATGAAGAAGG TGCTGGTGGA CAGCCCCAAG 
AAGCGGACGC TGCTCATCGA GAACCTGCGC GAGTCCCAGC CATACCGCTA CACGGTCAAG 
GCTCGCAACG GGGCAGGCTG GGGGCCTGAG CGGGAGGCCA TCATCAACCT GGCCACCCAG 
CCCAAGCGAC CCATGTCCAT CCCCATCATC CCTGACATCC CCATCGTGGA CGCCCAGAGT 
GGGGAGGACT ATGAGAGCTT CCTCATGTAC AGCGATGATG TGCTCCGCTC TCCAGCCGGC 
AGCCAGAGGC CCAGCGTCTC TGATGACACC GAGCACCTGG TGAACGGGCG GATGGACTTT 
GCCTTCCCGG GCAGCGCCAA CTCCCTGCAC AGGATGACCG TGAGCACCGC GGCCCACGGC 
GCCCACCTGA GCCCGCAGTT GTCCCACCGC GTGCTGAGCA CCTCGTCCAC CCTCACGCGG 
GACTACCACT CGCTGACCCG CACGGAGCAC TCGCACTCCG CCACGCTGCC CAGGGACTAC 
TCCACTCTCA CCTCCCTTTC CTCCCAAAGC TCCCGCTTGG CTGCGGGTGT GCCCAACACA 
CCCACCCGCC TGGTGTTCTC CGCCCTTGGA CCCACGTCTC TGAAAGTGAG CTGGCAGGAG 
CCGCAGTGTG AGCGTGCGCT GCAGGGCTAC AGTGTGGAGT ACCAGCTGCT GAACGGCGGT 
GAGCTGTATC GCCTCAACAT CCCCAACCCC AGCCAGACCT CGGTGGTGGT GGAGGACCTC 
CTGCCCAACC ACTCCTACGT GTTCCGTGTG CGGGCCCAGA GCCAGGAGGG CTGGGGCCCG 
GAGCGTGAGG GTGTCATCAC CATTGAGTCA CAGGTGCACC CGCAGAGCCC GCTCTGCCCC 
CTGCCGGGCT CCGCCTTCAC TTTGAGCACA CCCAGCGCCC CGGGCCCGCT GGTGTTCACT 
GCCCTGAGCC CCGACTCGCT GCAGCTGAGC TGGGAGCGGC CCCGCAGGCC AGATGGCGAC 
ATTCTGGGCT ACCTGGTGAC GTGTGAGATG GCCCACGGAG GAGGTCCAGC CACTACGTTC 
CTGGTTGACG GCGACAGCCC TGAGAGCCGG CTGACCGTGC CTGGCCTCAG CGAGAACGTG 
CCCTACAAGT TCAAGGTGCA GGCCAAGACC ACCCAAGGCT TCGGGCCGGA GCGAGAGGGA 
ATCATCACCA TCGAGTCCCA GGATGGAGGA CCCTTTCCAC AGCTGGGCGG CCACTTGGGG 
CTCTTCCAGC ACCCAGCGTC AGGGGAATAC AGCAGCATCA CCACCCACTC CAGCACCACT 
GAACCCTTCC TACTGGATGG ACTGACCCTG GGCTCCCAGC ATCTGGAAGC AAGTGGCTCC 
CTCACCCGGC ATGTGACACA GGAGTTTGTG AGCCGGACTC TGACCACCAG TGGGACCCTC 
AGCACCCACG TGGACCAACA GTTCTTCCAG ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180186.2
CDS	203..5458
Translation	MAGPHPSPWTRLLLAALLSVSFPGHMANRCKKAQVKSCTECIRVDKDCAYCTDEVFKERRCNTQAELLAAGCRLESVVVMESSFEITEERQIDTTLRRSQVSPQALRVRLRPGEERHFELQVFEPLESPMDLYILMDFSNSMSDDLDNLKKMGQDLAQVLRQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTEDVEEFRNKLKGERISGNLDAPEGGFDAILQTAVCTSDIGWRPDSTHLLVFSTESAFHYEADGANVLAGIMRRNDEECHLDPTGTYTQYKMQDYPSVPTLVRLLGQHNIIPIFAVTNYSYSYYERLSSYFPVSSLGVLQEDSSNIVDLLQEAFNRIRSNLDIRALDSPRGLRTEVTSRMFQKTDTGSFFIRRGEVGTYQVQLRAIEDLDGAHVCQLLEADQKGTIHLKPSFSNGLRMDVGVICDVCSCELQKEERSSRCNFHGDFMCGHCVCHEGWSGKTCNCSTGSQSDLQPCRREGEDKVCSGRGECQCGHCVCYGEGRYEGQFCEYDNFQCPRTSGFLCNDRGRCSMGQCVCEPGWTGLSCDCPLSNATCIDSNGGLCNGRGHCECGRCHCNQQSLYTDTVCEINYSAIHLGLCEDLRSCVQCQAWGTGEKKGRTCEECSFKVKMVDELKKAEEVVEHCSFRDEDDDCTYSYTVEGDSAPGPNSTVLVQRRKECPPGTFWWLIPLLIFLLLFPVLLLLLCWKYCACCKACLALLPCCNRGHMVGFKEDHYMLRENLMASDHLDTPLLRSGNLKGRDTVRWKITNNVQRPGFASHAAGINPSELVPYGLSLRLARLCTENLLKPGTRECDQLRQEVEENLNEVYRQITGAHNLQQTKFRQQPNAGKKQDHTIVDTVLMAPRSAKQALLKLTEKHVEQGAFHELKVAPGYYTLTADQDARGMVEFQEGVELVDVRVPLFIRPEDDDEKQLLVEAIDVPVGTATLGRRLVNITIIKEQASGIVSFEQPEYLVSSGEHVARIPVVRRILDSGKSQVSYRTQDNTAKANRDYIPMEGELLFQPGETWKELQVKLLELQEMDSLLRGPQTRRFYIQLSNPKFGARLGQPQCATVITGDRDELDRNVMNQTVSSPPPPRGDLGAPQNPNAKAAGSRKIHFNWLPPPGKPTGYRVKYWIQGDSESEAHLLDSKVPSVELTNLYPYCDYEMKVCAYGAQGEGPYSSPVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYGLVNEDNRPIGPMKKVLVDSPKKRTLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDIPIVDAQSGEDYESFLMYSDDVLRSPAGSQRPSVSDDTEHLVNGRMDFAFPGSANSLHRMTVSTAAHGAHLSPQLSHRVLSTSSTLTRDYHSLTRTEHSHSATLPRDYSTLTSLSSQSSRLAAGVPNTPTRLVFSALGPTSLKVSWQEPQCERALQGYSVEYQLLNGGELYRLNIPNPSQTSVVVEDLLPNHSYVFRVRAQSQEGWGPEREGVITIESQVHPQSPLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPDGDILGYLVTCEMAHGGGPATTFLVDGDSPESRLTVPGLSENVPYKFKVQAKTTQGFGPEREGIITIESQDGGPFPQLGGHLGLFQHPASGEYSSITTHSSTTEPFLLDGLTLGSQHLEASGSLTRHVTQEFVSRTLTTSGTLSTHVDQQFFQT

Target ORF information:

RefSeq Version	NM_001193257.2
Organism	Bos taurus(cattle)
Definition	Bos taurus integrin, beta 4 (ITGB4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193257.2

ORF Insert Sequence:

ATGGCAGGGC CACATCCCAG CCCGTGGACC AGGCTGCTGC TGGCAGCGCT GCTGAGCGTC 
AGCTTCCCCG GGCACATGGC GAACCGCTGC AAGAAGGCCC AGGTGAAGAG CTGTACCGAG 
TGCATCCGCG TGGACAAGGA CTGCGCCTAC TGCACGGACG AGGTGTTCAA GGAACGGCGC 
TGTAACACCC AGGCCGAGCT GCTGGCTGCC GGCTGCCGGC TGGAGAGCGT GGTGGTCATG 
GAGAGCAGCT TCGAGATCAC GGAGGAAAGG CAGATCGACA CCACGCTGCG CCGCAGTCAG 
GTGTCCCCCC AGGCGCTGCG GGTGCGGCTG CGGCCGGGCG AGGAGCGGCA CTTCGAGCTG 
CAGGTGTTCG AGCCCCTGGA GAGCCCCATG GACCTGTATA TCCTCATGGA CTTCTCCAAC 
TCCATGTCTG ATGATCTGGA CAACCTCAAG AAGATGGGGC AAGATCTGGC TCAGGTCCTG 
AGGCAGCTCA CCAGTGACTA CACAATAGGA TTTGGCAAGT TCGTGGACAA AGTCAGCGTC 
CCTCAGACAG ACATGAGGCC TGAGAAGCTG AAGGAACCCT GGCCCAACAG CGACCCCCCC 
TTCTCCTTCA AGAATGTCAT CAGCCTGACG GAAGACGTGG AGGAGTTCCG GAATAAGCTG 
AAGGGGGAGC GCATCTCAGG CAACCTGGAT GCCCCGGAAG GAGGTTTTGA TGCCATCCTG 
CAGACGGCCG TGTGCACCAG TGACATTGGC TGGCGCCCTG ACAGCACCCA CTTGCTGGTG 
TTCTCCACCG AGTCAGCCTT CCACTACGAG GCCGACGGGG CCAACGTGCT GGCCGGCATC 
ATGAGGCGTA ACGACGAGGA ATGCCACCTG GACCCCACGG GCACCTACAC CCAGTACAAG 
ATGCAGGACT ACCCGTCGGT GCCCACCCTG GTGCGCCTGC TCGGCCAACA CAACATCATC 
CCCATCTTCG CCGTCACCAA CTACTCCTAT AGCTACTATG AGAGGCTGTC GTCATATTTC 
CCCGTCTCTT CACTGGGGGT GCTGCAGGAG GACTCGTCCA ACATTGTGGA TCTGCTGCAG 
GAAGCCTTCA ACCGCATCCG CTCCAACCTG GACATTCGGG CCCTGGACAG TCCTCGAGGC 
CTGCGGACAG AGGTCACCTC TAGGATGTTC CAGAAGACGG ACACGGGGTC CTTTTTCATC 
CGGCGAGGGG AAGTGGGCAC ATACCAAGTG CAGCTCCGGG CCATCGAGGA CCTGGACGGG 
GCTCATGTGT GTCAGCTTCT GGAGGCAGAC CAGAAGGGCA CCATCCATCT GAAGCCATCC 
TTCTCCAACG GCCTCCGGAT GGACGTGGGC GTCATCTGTG ACGTGTGCTC CTGTGAGCTG 
CAAAAAGAGG AGCGGTCATC TCGCTGCAAC TTCCACGGGG ACTTCATGTG CGGACACTGT 
GTGTGCCACG AGGGCTGGAG CGGCAAGACC TGTAACTGCT CCACGGGCTC CCAGAGCGAC 
CTGCAGCCCT GCCGGCGGGA GGGCGAGGAC AAGGTGTGCT CCGGGCGGGG CGAGTGCCAG 
TGTGGCCACT GTGTGTGCTA TGGCGAAGGC CGCTACGAGG GTCAGTTCTG CGAATACGAC 
AACTTCCAGT GTCCCCGCAC CTCCGGGTTC CTCTGCAATG ACCGGGGACG CTGCTCCATG 
GGTCAGTGCG TGTGTGAGCC TGGCTGGACA GGCCTGAGCT GTGACTGTCC TCTCAGCAAT 
GCCACCTGCA TCGACAGTAA CGGGGGTCTC TGTAATGGGC GTGGCCACTG CGAGTGCGGC 
CGCTGCCACT GCAACCAACA GTCGCTCTAC ACGGACACCG TCTGCGAGAT CAACTACTCG 
GCGATCCACC TGGGCCTCTG TGAGGACCTG CGCTCCTGCG TGCAGTGCCA GGCCTGGGGC 
ACCGGTGAAA AGAAGGGGCG CACGTGCGAG GAGTGCAGCT TCAAGGTCAA GATGGTGGAT 
GAGCTCAAGA AAGCGGAGGA GGTGGTGGAG CACTGCTCCT TCCGGGACGA GGACGACGAC 
TGTACCTACA GTTACACCGT GGAGGGGGAC AGCGCCCCCG GGCCCAACAG CACCGTCTTG 
GTGCAGAGGA GGAAGGAATG CCCCCCGGGC ACCTTCTGGT GGCTCATCCC CCTGCTCATC 
TTCCTCCTCC TGTTCCCGGT CCTGCTGCTG CTGCTCTGCT GGAAGTACTG TGCCTGCTGC 
AAGGCCTGCC TGGCCCTTCT CCCTTGCTGC AACCGAGGTC ACATGGTGGG CTTCAAGGAA 
GACCATTACA TGCTGCGAGA GAACCTGATG GCCTCGGACC ACTTGGACAC GCCCTTGCTG 
CGCAGCGGGA ACCTCAAGGG TCGCGACACA GTCCGATGGA AGATCACCAA CAACGTGCAG 
CGGCCGGGCT TCGCCTCGCA CGCTGCAGGC ATCAACCCCT CAGAGCTGGT GCCCTACGGA 
CTGTCCCTGC GGCTTGCTCG CCTCTGCACC GAGAACCTGC TGAAGCCTGG CACGCGAGAA 
TGCGACCAGC TGCGCCAGGA GGTGGAGGAG AATCTGAACG AGGTGTACCG ACAAATCACA 
GGCGCTCACA ACCTCCAGCA GACCAAGTTC CGGCAGCAGC CCAACGCCGG GAAAAAGCAG 
GACCACACCA TTGTGGACAC TGTGCTGATG GCTCCCCGCT CGGCCAAGCA GGCCCTGCTG 
AAGCTGACGG AGAAGCACGT GGAGCAGGGG GCCTTCCATG AGCTCAAGGT GGCCCCCGGC 
TATTACACGC TCACCGCAGA CCAGGATGCC CGGGGCATGG TGGAATTCCA GGAGGGCGTG 
GAGCTGGTGG ACGTGCGGGT GCCTTTGTTT ATCCGGCCCG AGGACGACGA CGAGAAGCAG 
CTGCTGGTGG AGGCCATCGA CGTGCCTGTG GGCACCGCCA CCCTCGGACG TCGCCTGGTC 
AATATCACCA TCATCAAGGA GCAAGCCAGC GGGATCGTGT CCTTTGAGCA GCCCGAGTAC 
TTGGTCAGCA GCGGGGAGCA CGTTGCCCGC ATCCCCGTGG TCCGGCGCAT CCTGGACAGC 
GGCAAATCAC AGGTCTCCTA CCGCACACAG GATAACACCG CCAAGGCCAA CCGGGACTAC 
ATCCCCATGG AGGGTGAGCT GCTGTTCCAA CCTGGGGAGA CCTGGAAGGA GCTGCAGGTG 
AAGCTCCTGG AGCTGCAGGA GATGGACTCC CTTCTGCGGG GCCCCCAGAC CCGCCGCTTC 
TACATCCAAC TCAGCAACCC CAAGTTCGGG GCCCGCCTGG GCCAGCCCCA GTGTGCCACT 
GTCATCACTG GGGACCGAGA CGAGCTGGAC CGGAATGTAA TGAACCAGAC CGTGTCATCA 
CCACCACCGC CCCGGGGTGA CCTGGGTGCC CCACAGAACC CCAATGCCAA GGCTGCTGGG 
TCCCGGAAGA TCCACTTCAA CTGGCTGCCT CCTCCTGGCA AGCCGACAGG GTACCGGGTG 
AAATATTGGA TCCAGGGTGA CTCCGAGTCT GAAGCCCACC TGCTTGACAG CAAGGTGCCC 
TCAGTGGAGC TCACCAACCT GTACCCATAT TGTGACTACG AGATGAAGGT GTGCGCCTAT 
GGGGCGCAGG GCGAGGGCCC CTACAGCTCC CCGGTGTCCT GTCGCACCCA CCAGGAAGTG 
CCCAGCGAGC CCGGGCGTCT GGCCTTCAAC GTCGTCTCCT CCACCGTGAC CCAGCTGAGC 
TGGGCCGAGC CGGCTGAGAC CAACGGCGAG ATCACAGCCT ACGAGGTCTG CTATGGCCTG 
GTCAATGAGG ACAACCGACC CATCGGGCCC ATGAAGAAGG TGCTGGTGGA CAGCCCCAAG 
AAGCGGACGC TGCTCATCGA GAACCTGCGC GAGTCCCAGC CATACCGCTA CACGGTCAAG 
GCTCGCAACG GGGCAGGCTG GGGGCCTGAG CGGGAGGCCA TCATCAACCT GGCCACCCAG 
CCCAAGCGAC CCATGTCCAT CCCCATCATC CCTGACATCC CCATCGTGGA CGCCCAGAGT 
GGGGAGGACT ATGAGAGCTT CCTCATGTAC AGCGATGATG TGCTCCGCTC TCCAGCCGGC 
AGCCAGAGGC CCAGCGTCTC TGATGACACC GAGCACCTGG TGAACGGGCG GATGGACTTT 
GCCTTCCCGG GCAGCGCCAA CTCCCTGCAC AGGATGACCG TGAGCACCGC GGCCCACGGC 
GCCCACCTGA GCCCGCAGTT GTCCCACCGC GTGCTGAGCA CCTCGTCCAC CCTCACGCGG 
GACTACCACT CGCTGACCCG CACGGAGCAC TCGCACTCCG CCACGCTGCC CAGGGACTAC 
TCCACTCTCA CCTCCCTTTC CTCCCAAAGC TCCCGCTTGG CTGCGGGTGT GCCCAACACA 
CCCACCCGCC TGGTGTTCTC CGCCCTTGGA CCCACGTCTC TGAAAGTGAG CTGGCAGGAG 
CCGCAGTGTG AGCGTGCGCT GCAGGGCTAC AGTGTGGAGT ACCAGCTGCT GAACGGCGGT 
GAGCTGTATC GCCTCAACAT CCCCAACCCC AGCCAGACCT CGGTGGTGGT GGAGGACCTC 
CTGCCCAACC ACTCCTACGT GTTCCGTGTG CGGGCCCAGA GCCAGGAGGG CTGGGGCCCG 
GAGCGTGAGG GTGTCATCAC CATTGAGTCA CAGGTGCACC CGCAGAGCCC GCTCTGCCCC 
CTGCCGGGCT CCGCCTTCAC TTTGAGCACA CCCAGCGCCC CGGGCCCGCT GGTGTTCACT 
GCCCTGAGCC CCGACTCGCT GCAGCTGAGC TGGGAGCGGC CCCGCAGGCC AGATGGCGAC 
ATTCTGGGCT ACCTGGTGAC GTGTGAGATG GCCCACGGAG GAGGTCCAGC CACTACGTTC 
CTGGTTGACG GCGACAGCCC TGAGAGCCGG CTGACCGTGC CTGGCCTCAG CGAGAACGTG 
CCCTACAAGT TCAAGGTGCA GGCCAAGACC ACCCAAGGCT TCGGGCCGGA GCGAGAGGGA 
ATCATCACCA TCGAGTCCCA GGATGGAGGA CCCTTTCCAC AGCTGGGCGG CCACTTGGGG 
CTCTTCCAGC ACCCAGCGTC AGGGGAATAC AGCAGCATCA CCACCCACTC CAGCACCACT 
GAACCCTTCC TACTGGATGG ACTGACCCTG GGCTCCCAGC ATCTGGAAGC AAGTGGCTCC 
CTCACCCGGC ATGTGACACA GGAGTTTGTG AGCCGGACTC TGACCACCAG TGGGACCCTC 
AGCACCCACG TGGACCAACA GTTCTTCCAG ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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ITGB4 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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