itgb4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	itgb4
Entrez Gene ID	335269
Full Name	integrin, beta 4
Synonyms	fl03d06,wu:fb72e01,wu:fc27g01,wu:fl03d06,zgc:136313
General protein information	Preferred Names integrin, beta 4 Names integrin beta-4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005166790.4	XP_005166847.1	integrin beta-4 isoform X1
XM_005166791.3	XP_005166848.1	integrin beta-4 isoform X1
XM_005166790.3	XP_005166847.1	integrin beta-4 isoform X1
NM_001024386.1	NP_001019557.1	integrin beta-4 precursor
XM_005166791.4	XP_005166848.1	integrin beta-4 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ITGB4	NP_001180186.2
Danio rerio (zebrafish)	itgb4	NP_001019557.1
Pan troglodytes (chimpanzee)	ITGB4	XP_003315785.1
Rattus norvegicus (Norway rat)	Itgb4	NP_037312.1
Xenopus tropicalis (tropical clawed frog)	itgb4	XP_004918514.1
Canis lupus familiaris (dog)	ITGB4	XP_005624218.1
Gallus gallus (chicken)	ITGB4	XP_003642374.2
Homo sapiens (human)	ITGB4	NP_000204.3
	ITGB4	XP_002800666.1
Mus musculus (house mouse)	Itgb4	NP_001005608.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Comparative transcriptomic profiling of hydrogen peroxide signaling networks in zebrafish and human keratinocytes: Implications toward conservation, migration and wound healing.
Lisse TS, King BL, Rieger S
Scientific reports620328(2016 Feb)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Zebrafish: a model system to study heritable skin diseases.
Li Q, Frank M, Thisse CI, Thisse BV, Uitto J
The Journal of investigative dermatology131(3)565-71(2011 Mar)

Nlcam modulates midline convergence during anterior neural plate morphogenesis.
Brown KE, Keller PJ, Ramialison M, Rembold M, Stelzer EH, Loosli F, Wittbrodt J
Developmental biology339(1)14-25(2010 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following itgb4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the itgb4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa33629	NM_001024386.1 Latest version!	Danio rerio integrin, beta 4 (itgb4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa13938	XM_005166790.4 Latest version!	Danio rerio integrin, beta 4 (itgb4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa13938	XM_005166791.4 Latest version!	Danio rerio integrin, beta 4 (itgb4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa13938	XM_005166790.3	Danio rerio integrin, beta 4 (itgb4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa13938	XM_005166791.3	Danio rerio integrin, beta 4 (itgb4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

itgb4 ( NM_001024386.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001019557.1 Danio rerio integrin, beta 4 (itgb4), mRNA.

Price & Availability↑

CloneID	ODa33629
Clone ID Related Accession (Same CDS sequence)	NM_001024386.1
Accession Version	NM_001024386.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	integrin beta-4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ022377.1. On or before Feb 16, 2011 this sequence version replaced XM_002663082.1, NM_001045266.1. ##Evidence-Data-START## Transcript exon combination :: DQ022377.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGGAGGAT GGGCGATACG TCTGGCTGTG GGGATCTTCC TTCTCTTGCT TGCTGCGACA 
GACAGTCGTG CTGATGAAGT GAATCACTGC ACTGCTGCCA GGACCAAAAC CTGCTCAGAA 
TGTATTCAGG TTGGCGCAGG ATGTGCATAC TGCCCTGATG AGGTCTTCGA GCATGAGCGT 
TGTGACCTGA AAGAAAACCT GGAGGCCCAA AAATGTATCA AAATAGTAAA TTTTGAGAGC 
AGCAGGCAAA TCCAAGAGAA TGTGAAAATC AACGAGCAAC TGAAGCAGTC TCAGGTGTCC 
CCTCAGCTCA TGAGCATGAC GCTTCTGCCC GGGGAGGAGA AAGAGGTGGA GATGGAGGTG 
TTTGAACCCA CCCGTGGCCC ACTGGATCTC TACATCCTCA TGGACTTCTC CAACTCCATG 
TCTGACGATC TGGACAACCT TAAGAGGATG GGAGAAAAGC TGGCACGGCT TGTTGGCGAT 
CTCTCTGACG ATTACACCAT CGGATTTGGC AAGTTTGTGG ACAAAGTCAC CGAGCCTCAG 
ACAGACATGA GGCCAGCCAA ATTAGCTGAG CCGTGGTCCA ATAGCGACCC TCCATTTTCC 
TTCCGTAACG TCATCAAACT CACCAGCAAC ATCACCTCCT TCAGACAGAA GCTACAGAAA 
GAGCGTATAT CTGGCAACCT TGATGCTCCA GAAGGTGGTT TTGATGCCAT CTTACAGACT 
GCTGTCTGCC AGGACCAAAT TGGCTGGAGG AAAGACAGCA CACACTTGCT GGTCTTCTCC 
ACTGAATCAG CTTTCCATTA TGAGGCAGAT GGTGCCAACG TGCTCGCTGG CATCTTGGAC 
CGCAATGATG AGCAATGCCA TCTGAATGTA GATGGAAACT ACACGCATGA TGTTCGTCAA 
GATTACCCAT CTATACCGAC TCTAGTGCGA CTTTTGGTGA AACATAACAT CATCCCAATT 
TTTGCTGTTA CTAATCACTC ATACTCATAC TATGAGAAAC TTCATGAATA CTTCCCCATT 
GCTGAGCTGG GACAGTTACA GGAGGATTCA TCTAATATCC TGAGCATTTT AGAAAAAGCT 
TTTGAGAATA TTCGTTCCAA GATCAGTATT CGTGCAGAGG ACAGACCAAA GGCAGTGGAA 
ACCAAGATTT TCTCTCAGTC TGGCACCTTT TCTGAATATG GCAATTTCAA AATCACACCA 
GGACAAACTG GTAAATTTAA AGTGCGTATG AAGGCCCTTA ATCAGGTAGG AGAAGAGCCC 
GTGTGTAAGG TCAACCAGGC TGACCGCGCA GGAACCTTGA GAGTCAAGCC CACCACCTTC 
AGCTCTGCCT TTAAAATCAA TGCAGAGGTG CTCTGTCCAA CCTGTGACTG TGAAAAGACC 
CCGGTTAAAA ATGCAATGCG CTGTACTGGC CATGGAGATC TGGTCTGTGG GAAATGCCAG 
TGCTATGCAG GATGGCTCGG CCCTTTGTGT AACTGTTCAG CAAGCGCCTC CACAGACTCT 
GTCATGTGCA TAGAACCAGG AAAGACAGAG CCGTGCTCAG GCAGAGGAGA CTGCATGTGT 
GGGACCTGTG TGTGTTATAA CCCCAACCAG TTTGAAGGGC CCTACTGCCA GTTTGACAAA 
TCTCAGTGCC AGAGATTTGG TGGATTTCTC TGTAATGAGC GTGGAAGCTG CAGCATGGGC 
AGATGTGTTT GTTCTCCGGG ATGGAGTGGA GAGGCCTGTG AATGTCCCAC CAGCAATGAC 
TCATGCCGGG ATAGCAAAGG GGGCATCTGT AATGACCGTG GTGTGTGTAA ATGTGGCCGC 
TGTGAGTGTG AACACTCAGG ACTTCCACTG AGTCCTACCT GCGAAGCCAA CTTTCAGCAA 
CAAGTGGGTA TGTGTGAAGC CAAGAGGAGC TGCGTCCAGT GCCAGGCCTG GAGCACCGGT 
GAAAGGAAAG GACGGAAATG CGATGAGTGT CCCTTCAAAA TTGTCAGGGT GGATGAGCTT 
GAAGAAGATA AAAATGTAAT CGAGACATGC AGTTTCCGTG ATGAAGATGA TGACTGCACA 
TATCACTATA ATGTGAATTA TCCATCGAAC ATCACAGACA AAGAACACCG CGTCCTAGTG 
AAGAAAAAGA AAGATTGTCC ACCTGCAGGT TTCCTGTGGC TCATTCCTCT CATTATGTTT 
CTGATGCTGT TACTTGGTTT GCTTTTGCTC TGTTGCTGGA AATACTGCGC CTGCTGCCAG 
TCCTGTCTTG CTCTTCTCCC GTGCTGTGCA AGAGGTCGTA TGGTGGGCTT TAAAGAAAAA 
CAGTACCTGC TACGTCAGTC TTTTCTGCAA AATGATTATT TGGACACCCC AATGGTGCGC 
AGCGGCCCGC TCAAGAGCAC AGACGTTGTG CGCTGGAAAG TGGCAGACAA TGTTCATCGT 
GGAACGAATC ACCCCCAGAA CCAAATCAGA CCCAACCCCA AAGAGACCAT CCAGTATCCT 
GTTTCTCTTC GTCTGAACCG CCAGTTTTCA GAAGTTCTTT CTAACCCTGA CGCTCGGGAC 
ACAGACGTGC TCAGACGAGA AGTAGAGGAC AACCTCAATG ACGTGTTCCT GCAGATCCCG 
GGCGCGCAAC CAGTGCAGAA GACTCTGTTC AGGACACAAA GAAATTCTGG AAAGAGACAG 
AACAACACAA TTGTGGACAC AGTTTTATCT GCCCCTCGCT CCAGTTACCC AGATATTGTA 
AAACTGACAG ATAAACAGGT TCAGTCTGGA AACTTCCGGG ACCTGAAGGT GATTCCAGGC 
TACTACACTG TGGCTTCTGA TAAAGAAGCA GCAGGCGCAG TGGAGTTTCC GGAGAACGTG 
GAATCAGTGG ATGTTCGGGT TCCTCTCTTC ATTAAAGATG AGGACGATGA AAAGAAGGAG 
CTGCTTGTGG AAGCTGTGGA TGTTCCAGTC GGAATTGCAA AGATTGGAAA AAATCATGTC 
AACATTACAG TGCTCAAGGA ACAGGCTTCG AGTGTCTTTA CCTTCCTGGA GCCGTCATAT 
ACCTACAGCC GTCAAGATGG AGTGGCCAAC ATCCCGATCA AACGTCAGAT CATAGAGGAT 
GGACGGGCAC AAGTCACATA CAGCACAGCA GACCTCACTG CTAAAGACAA GAAGGACTAT 
ATTTCGGTAG ATGGAGACTT GAGCTATGGA CAAGGTGAAA CAGAGAAGAT TGTCCCAGTG 
AAACTTCTGG AACTTGGAGA GCAAGATGGC CTGCTGGAGG ACAAGCAGGT CAAACAGTTT 
GTCATGGATT TGACCAACCC ACGACAAAGC GCTAAACTAG GCCGTTACCC ACGCACTGTT 
ATCACCATCG CTGACAAACC AGAATCCAGT GTTATGGCGT TTAAGAAGAG TACTCAAACT 
TTCAGTACCA CCGACTCGCT CTACACCATC CCGGTGGAAC GCACTGGAAA CCGGGAGACT 
CCGGCCACTG TGCACTGGCG CACCAACAAG GCCTCGCGCT TCGACATCTC CAGCCCTCTC 
AAATTTGCAC CCGGTGAAGC TGAAAAAAAT ATCCTCATCA ATCCTCGTAC ACATCCATCT 
CCCATCATAC CTGAGAAATT TAACCTGGAA CTATTAGACC CAAGCAACAA TGCAATTATC 
GGAAAGAGGA AGACCACGGA GGTGATTGTC ACCGACGGTC GAGGGGATGG TAAAAATCAG 
GATTTCCAGA AGATGGAATA TGTTACTCAA ACAGCCACAT CCCCCGGAGG ACGCCTCTAT 
TCTCCAGCCA ACATTAAAGC CGTTCCTACC GGCCCTAAAA ACATCCGCCT GAACTGGAAA 
CCCAGCCAAA ATGCCAATGG TTACAAGGTG AAGTACTGGA TTTATGGAGA CCCCGAAGAT 
AAAGCTAAAT TTATGGATGT GAAGACGCCT CAAGCCGAGC TGACAAATCT CGATCCATAC 
TGTGATTACG AGATGCGTGT TTGTGGCTAC AATGCACTTG GAGATGGCAA TTACAGTGAT 
ATCATCCAAT GCCAAACCCT GGAGGATATA CCTGGTGAAC CTGGTCGTCT GGCTTTCAAC 
GTCATCAGCC CTACTGTTAC TCAGGTTAGT TGGGCGGAGC CAGCAGAAGC CAATGGTATC 
ATCACTGGCT ATGAGGTCAT CTACATGCCC ATTAACGAGG ACAAGAAGCC TACAGGACCT 
CCTAAGAAGG TGATGATTGA CAACCCTAAA AAGCGCATGT TGCTCATTGA GAACCTGCAA 
CCTTCTCAGA CCTACTGTTA CAAAGTGCGT GCCAGCAATA AGGTGGGCTG GGGCCCATAC 
AGAGAAGCCA CAATCAACCT GGCATCACAG CCTACCAGAC CCATGTCAAT TCCCATCATT 
CCCGACATTC CCATCGTGGA TGCAGAAGCT GGCGAAGAGT ACGACAGCTA TCTGATGTAC 
AGTAATGAAG TCCTGCGATC CCCAACGAGC AGCCTGAGGC CTAGCGTCAC AGATCTGTCT 
GATGATCAGT TCGTGAACGG AAAGTGGGAA CAGAATTTCT TGTTTCCTGG AGGAGGAGGT 
GGTTCCCTCT CCCGCAACAT CAGCTCTTCC TCAACCACAT ACAGCCATTC CACACCGCGT 
ACCAATGTGA CCAACAACAG CTCCACCACA ACGTACATCT CTGGCAAAGG AGGCTCAACT 
ACACACAGAT ATGATGTAAG AGACGGTGGA CTTCTCAAAG AAGACGTGAT CCTGAGAAAA 
CGCTCTGAAA ACAGGCATTA CACAGAAGGT GATGGTGTGA GGGACTCAAT CGTAATGGGA 
AACCTGTCTG GTGGACTTTT GGACAATTTA GAATCGCGCT TCACCCCGGG TGTTCCCGCG 
ACTCCCAGCA GACTGGTGTT TTCAGCTCTG GGCCACACGG CTCTCAAAGT GAGCTGGCAG 
GAGCCACACT GCGAGTCTGA TGTACTGGGA TACTGCGTCC TTTACCAGCT CATCAATGGA 
GGTGACGTAA AACGTATCGA GGTGAACAAT CCTGCTCAGA ACTCAGTGCT GGTTCAGGAT 
CTCCTGCCTA ACCAGTCCTA CATCTTCAAA GTCAAAGCAC AGAGTCGAGA GGGCTGGGGT 
CCTGAGAGAG AGGGCGTCAT TACCATTGAG TCCAACGTCG ACCCCAAGAG TCCACTGAGC 
CCAGTGCCCG GGTCTCCGTT CACTCTCAGC ACTCCCAGCG CTCCAGGACC TCTGATCTTC 
ACAGCGTTAC GTCCAGATAC ACTGCAGCTG AGCTGGGAGA AACCCCGAAA ACCCAACGGA 
GACATCCTGG GATACGTGGT GACCTGCGAA CAGCTGCATG GTGGARGAGA TCTTCGCTCC 
TTCCAGGTGA ATGGCAATGC AGCCACTTCA TTGACTGTCT CTGACCTGAA AGAAAATATG 
CCTTACAAAT TCAAAGTTCA AGCTCAGACC ACGCAGGGCT TTGGCCCAGA GCGAGAGGGA 
ATCATCACCA TCGAGTCCCA GGATGGAGGC TCCATGTCCC AATACAGCAG TCAGTCGGTC 
ACGAAGAGAG AGGTGTTCAA TCTGCCCACA GAAATCACAC ACACTTCACA CACCATGTTC 
TCAGAGCCCT TTGTTTCTTC AGACGGGATG ATGATGATGT CGAGCAGGCA GATCACAGAG 
TCGGGTGGCT CCATGACACG GCAGGTGGAG ATGGTGCAGA GAGGAGTGAT GTCCAGCAGC 
ACCGTCACCA AGAGGCTGGA GAGACAGTAC TACGAGGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019557.1
CDS	162..5843
Translation	MGGWAIRLAVGIFLLLLAATDSRADEVNHCTAARTKTCSECIQVGAGCAYCPDEVFEHERCDLKENLEAQKCIKIVNFESSRQIQENVKINEQLKQSQVSPQLMSMTLLPGEEKEVEMEVFEPTRGPLDLYILMDFSNSMSDDLDNLKRMGEKLARLVGDLSDDYTIGFGKFVDKVTEPQTDMRPAKLAEPWSNSDPPFSFRNVIKLTSNITSFRQKLQKERISGNLDAPEGGFDAILQTAVCQDQIGWRKDSTHLLVFSTESAFHYEADGANVLAGILDRNDEQCHLNVDGNYTHDVRQDYPSIPTLVRLLVKHNIIPIFAVTNHSYSYYEKLHEYFPIAELGQLQEDSSNILSILEKAFENIRSKISIRAEDRPKAVETKIFSQSGTFSEYGNFKITPGQTGKFKVRMKALNQVGEEPVCKVNQADRAGTLRVKPTTFSSAFKINAEVLCPTCDCEKTPVKNAMRCTGHGDLVCGKCQCYAGWLGPLCNCSASASTDSVMCIEPGKTEPCSGRGDCMCGTCVCYNPNQFEGPYCQFDKSQCQRFGGFLCNERGSCSMGRCVCSPGWSGEACECPTSNDSCRDSKGGICNDRGVCKCGRCECEHSGLPLSPTCEANFQQQVGMCEAKRSCVQCQAWSTGERKGRKCDECPFKIVRVDELEEDKNVIETCSFRDEDDDCTYHYNVNYPSNITDKEHRVLVKKKKDCPPAGFLWLIPLIMFLMLLLGLLLLCCWKYCACCQSCLALLPCCARGRMVGFKEKQYLLRQSFLQNDYLDTPMVRSGPLKSTDVVRWKVADNVHRGTNHPQNQIRPNPKETIQYPVSLRLNRQFSEVLSNPDARDTDVLRREVEDNLNDVFLQIPGAQPVQKTLFRTQRNSGKRQNNTIVDTVLSAPRSSYPDIVKLTDKQVQSGNFRDLKVIPGYYTVASDKEAAGAVEFPENVESVDVRVPLFIKDEDDEKKELLVEAVDVPVGIAKIGKNHVNITVLKEQASSVFTFLEPSYTYSRQDGVANIPIKRQIIEDGRAQVTYSTADLTAKDKKDYISVDGDLSYGQGETEKIVPVKLLELGEQDGLLEDKQVKQFVMDLTNPRQSAKLGRYPRTVITIADKPESSVMAFKKSTQTFSTTDSLYTIPVERTGNRETPATVHWRTNKASRFDISSPLKFAPGEAEKNILINPRTHPSPIIPEKFNLELLDPSNNAIIGKRKTTEVIVTDGRGDGKNQDFQKMEYVTQTATSPGGRLYSPANIKAVPTGPKNIRLNWKPSQNANGYKVKYWIYGDPEDKAKFMDVKTPQAELTNLDPYCDYEMRVCGYNALGDGNYSDIIQCQTLEDIPGEPGRLAFNVISPTVTQVSWAEPAEANGIITGYEVIYMPINEDKKPTGPPKKVMIDNPKKRMLLIENLQPSQTYCYKVRASNKVGWGPYREATINLASQPTRPMSIPIIPDIPIVDAEAGEEYDSYLMYSNEVLRSPTSSLRPSVTDLSDDQFVNGKWEQNFLFPGGGGGSLSRNISSSSTTYSHSTPRTNVTNNSSTTTYISGKGGSTTHRYDVRDGGLLKEDVILRKRSENRHYTEGDGVRDSIVMGNLSGGLLDNLESRFTPGVPATPSRLVFSALGHTALKVSWQEPHCESDVLGYCVLYQLINGGDVKRIEVNNPAQNSVLVQDLLPNQSYIFKVKAQSREGWGPEREGVITIESNVDPKSPLSPVPGSPFTLSTPSAPGPLIFTALRPDTLQLSWEKPRKPNGDILGYVVTCEQLHGGnullDLRSFQVNGNAATSLTVSDLKENMPYKFKVQAQTTQGFGPEREGIITIESQDGGSMSQYSSQSVTKREVFNLPTEITHTSHTMFSEPFVSSDGMMMMSSRQITESGGSMTRQVEMVQRGVMSSSTVTKRLERQYYEA

Target ORF information:

RefSeq Version	NM_001024386.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio integrin, beta 4 (itgb4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024386.1

ORF Insert Sequence:

ATGGGAGGAT GGGCGATACG TCTGGCTGTG GGGATCTTCC TTCTCTTGCT TGCTGCGACA 
GACAGTCGTG CTGATGAAGT GAATCACTGC ACTGCTGCCA GGACCAAAAC CTGCTCAGAA 
TGTATTCAGG TTGGCGCAGG ATGTGCATAC TGCCCTGATG AGGTCTTCGA GCATGAGCGT 
TGTGACCTGA AAGAAAACCT GGAGGCCCAA AAATGTATCA AAATAGTAAA TTTTGAGAGC 
AGCAGGCAAA TCCAAGAGAA TGTGAAAATC AACGAGCAAC TGAAGCAGTC TCAGGTGTCC 
CCTCAGCTCA TGAGCATGAC GCTTCTGCCC GGGGAGGAGA AAGAGGTGGA GATGGAGGTG 
TTTGAACCCA CCCGTGGCCC ACTGGATCTC TACATCCTCA TGGACTTCTC CAACTCCATG 
TCTGACGATC TGGACAACCT TAAGAGGATG GGAGAAAAGC TGGCACGGCT TGTTGGCGAT 
CTCTCTGACG ATTACACCAT CGGATTTGGC AAGTTTGTGG ACAAAGTCAC CGAGCCTCAG 
ACAGACATGA GGCCAGCCAA ATTAGCTGAG CCGTGGTCCA ATAGCGACCC TCCATTTTCC 
TTCCGTAACG TCATCAAACT CACCAGCAAC ATCACCTCCT TCAGACAGAA GCTACAGAAA 
GAGCGTATAT CTGGCAACCT TGATGCTCCA GAAGGTGGTT TTGATGCCAT CTTACAGACT 
GCTGTCTGCC AGGACCAAAT TGGCTGGAGG AAAGACAGCA CACACTTGCT GGTCTTCTCC 
ACTGAATCAG CTTTCCATTA TGAGGCAGAT GGTGCCAACG TGCTCGCTGG CATCTTGGAC 
CGCAATGATG AGCAATGCCA TCTGAATGTA GATGGAAACT ACACGCATGA TGTTCGTCAA 
GATTACCCAT CTATACCGAC TCTAGTGCGA CTTTTGGTGA AACATAACAT CATCCCAATT 
TTTGCTGTTA CTAATCACTC ATACTCATAC TATGAGAAAC TTCATGAATA CTTCCCCATT 
GCTGAGCTGG GACAGTTACA GGAGGATTCA TCTAATATCC TGAGCATTTT AGAAAAAGCT 
TTTGAGAATA TTCGTTCCAA GATCAGTATT CGTGCAGAGG ACAGACCAAA GGCAGTGGAA 
ACCAAGATTT TCTCTCAGTC TGGCACCTTT TCTGAATATG GCAATTTCAA AATCACACCA 
GGACAAACTG GTAAATTTAA AGTGCGTATG AAGGCCCTTA ATCAGGTAGG AGAAGAGCCC 
GTGTGTAAGG TCAACCAGGC TGACCGCGCA GGAACCTTGA GAGTCAAGCC CACCACCTTC 
AGCTCTGCCT TTAAAATCAA TGCAGAGGTG CTCTGTCCAA CCTGTGACTG TGAAAAGACC 
CCGGTTAAAA ATGCAATGCG CTGTACTGGC CATGGAGATC TGGTCTGTGG GAAATGCCAG 
TGCTATGCAG GATGGCTCGG CCCTTTGTGT AACTGTTCAG CAAGCGCCTC CACAGACTCT 
GTCATGTGCA TAGAACCAGG AAAGACAGAG CCGTGCTCAG GCAGAGGAGA CTGCATGTGT 
GGGACCTGTG TGTGTTATAA CCCCAACCAG TTTGAAGGGC CCTACTGCCA GTTTGACAAA 
TCTCAGTGCC AGAGATTTGG TGGATTTCTC TGTAATGAGC GTGGAAGCTG CAGCATGGGC 
AGATGTGTTT GTTCTCCGGG ATGGAGTGGA GAGGCCTGTG AATGTCCCAC CAGCAATGAC 
TCATGCCGGG ATAGCAAAGG GGGCATCTGT AATGACCGTG GTGTGTGTAA ATGTGGCCGC 
TGTGAGTGTG AACACTCAGG ACTTCCACTG AGTCCTACCT GCGAAGCCAA CTTTCAGCAA 
CAAGTGGGTA TGTGTGAAGC CAAGAGGAGC TGCGTCCAGT GCCAGGCCTG GAGCACCGGT 
GAAAGGAAAG GACGGAAATG CGATGAGTGT CCCTTCAAAA TTGTCAGGGT GGATGAGCTT 
GAAGAAGATA AAAATGTAAT CGAGACATGC AGTTTCCGTG ATGAAGATGA TGACTGCACA 
TATCACTATA ATGTGAATTA TCCATCGAAC ATCACAGACA AAGAACACCG CGTCCTAGTG 
AAGAAAAAGA AAGATTGTCC ACCTGCAGGT TTCCTGTGGC TCATTCCTCT CATTATGTTT 
CTGATGCTGT TACTTGGTTT GCTTTTGCTC TGTTGCTGGA AATACTGCGC CTGCTGCCAG 
TCCTGTCTTG CTCTTCTCCC GTGCTGTGCA AGAGGTCGTA TGGTGGGCTT TAAAGAAAAA 
CAGTACCTGC TACGTCAGTC TTTTCTGCAA AATGATTATT TGGACACCCC AATGGTGCGC 
AGCGGCCCGC TCAAGAGCAC AGACGTTGTG CGCTGGAAAG TGGCAGACAA TGTTCATCGT 
GGAACGAATC ACCCCCAGAA CCAAATCAGA CCCAACCCCA AAGAGACCAT CCAGTATCCT 
GTTTCTCTTC GTCTGAACCG CCAGTTTTCA GAAGTTCTTT CTAACCCTGA CGCTCGGGAC 
ACAGACGTGC TCAGACGAGA AGTAGAGGAC AACCTCAATG ACGTGTTCCT GCAGATCCCG 
GGCGCGCAAC CAGTGCAGAA GACTCTGTTC AGGACACAAA GAAATTCTGG AAAGAGACAG 
AACAACACAA TTGTGGACAC AGTTTTATCT GCCCCTCGCT CCAGTTACCC AGATATTGTA 
AAACTGACAG ATAAACAGGT TCAGTCTGGA AACTTCCGGG ACCTGAAGGT GATTCCAGGC 
TACTACACTG TGGCTTCTGA TAAAGAAGCA GCAGGCGCAG TGGAGTTTCC GGAGAACGTG 
GAATCAGTGG ATGTTCGGGT TCCTCTCTTC ATTAAAGATG AGGACGATGA AAAGAAGGAG 
CTGCTTGTGG AAGCTGTGGA TGTTCCAGTC GGAATTGCAA AGATTGGAAA AAATCATGTC 
AACATTACAG TGCTCAAGGA ACAGGCTTCG AGTGTCTTTA CCTTCCTGGA GCCGTCATAT 
ACCTACAGCC GTCAAGATGG AGTGGCCAAC ATCCCGATCA AACGTCAGAT CATAGAGGAT 
GGACGGGCAC AAGTCACATA CAGCACAGCA GACCTCACTG CTAAAGACAA GAAGGACTAT 
ATTTCGGTAG ATGGAGACTT GAGCTATGGA CAAGGTGAAA CAGAGAAGAT TGTCCCAGTG 
AAACTTCTGG AACTTGGAGA GCAAGATGGC CTGCTGGAGG ACAAGCAGGT CAAACAGTTT 
GTCATGGATT TGACCAACCC ACGACAAAGC GCTAAACTAG GCCGTTACCC ACGCACTGTT 
ATCACCATCG CTGACAAACC AGAATCCAGT GTTATGGCGT TTAAGAAGAG TACTCAAACT 
TTCAGTACCA CCGACTCGCT CTACACCATC CCGGTGGAAC GCACTGGAAA CCGGGAGACT 
CCGGCCACTG TGCACTGGCG CACCAACAAG GCCTCGCGCT TCGACATCTC CAGCCCTCTC 
AAATTTGCAC CCGGTGAAGC TGAAAAAAAT ATCCTCATCA ATCCTCGTAC ACATCCATCT 
CCCATCATAC CTGAGAAATT TAACCTGGAA CTATTAGACC CAAGCAACAA TGCAATTATC 
GGAAAGAGGA AGACCACGGA GGTGATTGTC ACCGACGGTC GAGGGGATGG TAAAAATCAG 
GATTTCCAGA AGATGGAATA TGTTACTCAA ACAGCCACAT CCCCCGGAGG ACGCCTCTAT 
TCTCCAGCCA ACATTAAAGC CGTTCCTACC GGCCCTAAAA ACATCCGCCT GAACTGGAAA 
CCCAGCCAAA ATGCCAATGG TTACAAGGTG AAGTACTGGA TTTATGGAGA CCCCGAAGAT 
AAAGCTAAAT TTATGGATGT GAAGACGCCT CAAGCCGAGC TGACAAATCT CGATCCATAC 
TGTGATTACG AGATGCGTGT TTGTGGCTAC AATGCACTTG GAGATGGCAA TTACAGTGAT 
ATCATCCAAT GCCAAACCCT GGAGGATATA CCTGGTGAAC CTGGTCGTCT GGCTTTCAAC 
GTCATCAGCC CTACTGTTAC TCAGGTTAGT TGGGCGGAGC CAGCAGAAGC CAATGGTATC 
ATCACTGGCT ATGAGGTCAT CTACATGCCC ATTAACGAGG ACAAGAAGCC TACAGGACCT 
CCTAAGAAGG TGATGATTGA CAACCCTAAA AAGCGCATGT TGCTCATTGA GAACCTGCAA 
CCTTCTCAGA CCTACTGTTA CAAAGTGCGT GCCAGCAATA AGGTGGGCTG GGGCCCATAC 
AGAGAAGCCA CAATCAACCT GGCATCACAG CCTACCAGAC CCATGTCAAT TCCCATCATT 
CCCGACATTC CCATCGTGGA TGCAGAAGCT GGCGAAGAGT ACGACAGCTA TCTGATGTAC 
AGTAATGAAG TCCTGCGATC CCCAACGAGC AGCCTGAGGC CTAGCGTCAC AGATCTGTCT 
GATGATCAGT TCGTGAACGG AAAGTGGGAA CAGAATTTCT TGTTTCCTGG AGGAGGAGGT 
GGTTCCCTCT CCCGCAACAT CAGCTCTTCC TCAACCACAT ACAGCCATTC CACACCGCGT 
ACCAATGTGA CCAACAACAG CTCCACCACA ACGTACATCT CTGGCAAAGG AGGCTCAACT 
ACACACAGAT ATGATGTAAG AGACGGTGGA CTTCTCAAAG AAGACGTGAT CCTGAGAAAA 
CGCTCTGAAA ACAGGCATTA CACAGAAGGT GATGGTGTGA GGGACTCAAT CGTAATGGGA 
AACCTGTCTG GTGGACTTTT GGACAATTTA GAATCGCGCT TCACCCCGGG TGTTCCCGCG 
ACTCCCAGCA GACTGGTGTT TTCAGCTCTG GGCCACACGG CTCTCAAAGT GAGCTGGCAG 
GAGCCACACT GCGAGTCTGA TGTACTGGGA TACTGCGTCC TTTACCAGCT CATCAATGGA 
GGTGACGTAA AACGTATCGA GGTGAACAAT CCTGCTCAGA ACTCAGTGCT GGTTCAGGAT 
CTCCTGCCTA ACCAGTCCTA CATCTTCAAA GTCAAAGCAC AGAGTCGAGA GGGCTGGGGT 
CCTGAGAGAG AGGGCGTCAT TACCATTGAG TCCAACGTCG ACCCCAAGAG TCCACTGAGC 
CCAGTGCCCG GGTCTCCGTT CACTCTCAGC ACTCCCAGCG CTCCAGGACC TCTGATCTTC 
ACAGCGTTAC GTCCAGATAC ACTGCAGCTG AGCTGGGAGA AACCCCGAAA ACCCAACGGA 
GACATCCTGG GATACGTGGT GACCTGCGAA CAGCTGCATG GTGGARGAGA TCTTCGCTCC 
TTCCAGGTGA ATGGCAATGC AGCCACTTCA TTGACTGTCT CTGACCTGAA AGAAAATATG 
CCTTACAAAT TCAAAGTTCA AGCTCAGACC ACGCAGGGCT TTGGCCCAGA GCGAGAGGGA 
ATCATCACCA TCGAGTCCCA GGATGGAGGC TCCATGTCCC AATACAGCAG TCAGTCGGTC 
ACGAAGAGAG AGGTGTTCAA TCTGCCCACA GAAATCACAC ACACTTCACA CACCATGTTC 
TCAGAGCCCT TTGTTTCTTC AGACGGGATG ATGATGATGT CGAGCAGGCA GATCACAGAG 
TCGGGTGGCT CCATGACACG GCAGGTGGAG ATGGTGCAGA GAGGAGTGAT GTCCAGCAGC 
ACCGTCACCA AGAGGCTGGA GAGACAGTAC TACGAGGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itgb4 ( XM_005166790.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166847.1 Danio rerio integrin, beta 4 (itgb4), transcript variant X1, mRNA.

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CloneID	ODa13938
Clone ID Related Accession (Same CDS sequence)	XM_005166790.4 , XM_005166791.3 , XM_005166790.3 , XM_005166791.4
Accession Version	XM_005166790.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	integrin beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166790.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGGAT GGGCGATACG TCTGGCTGTG GGGATCTTCC TTCTCTTGCT TGCTGCGACA 
GACAGTCGTG CTGATGAAGT GAATCACTGC ACTGCTGCCA GGACCAAAAC CTGCTCAGAA 
TGTATTCAGG TTGGCGCAGG ATGTGCGTAC TGCCCTGATG AGGTCTTCGA GCATGAGCGT 
TGTGACCTGA AAGAAAACCT GGAGGCCCAA AAATGTAGCA AAATAGTAAA TTTTGAGAGC 
AGCAGGCAAA TCCAAGAGAA TGTGAAAATC AACGAGCAAC TGAAGCAGTC TCAGGTGTCC 
CCTCAGCTCA TGAGCATGAC GCTTCTGCCC GGGGAGGAGA AAGAGGTGGA GATGGAGGTG 
TTTGAACCCA CCCGTGGCCC ACTGGATCTC TACATCCTCA TGGACTTCTC CAACTCCATG 
TCTGACGATC TGGACAACCT TAAGAGGATG GGAGAAAAGC TGGCACGGCT TGTTGGCGAT 
CTCTCTGACG ATTACACCAT CGGATTTGGC AAGTTTGTGG ACAAAGTCAC CGAGCCTCAG 
ACAGACATGA GGCCAGCCAA ATTAGCTGAG CCGTGGTCCA ATAGCGACCC TCCATTTTCC 
TTCCGTAACG TCATCAAGCT CACCAGCAAC ATCACCTCCT TCAGACAGAA GCTACAGAAA 
GAGCGTATAT CTGGCAACCT TGATGCTCCA GAAGGTGGTT TTGATGCCAT CTTACAGACT 
GCTGTCTGCC AGGACCAAAT TGGCTGGAGG AAAGACAGCA CACACTTGCT GGTCTTCTCC 
ACTGAATCAG CTTTCCATTA TGAGGCAGAT GGTGCCAACG TGCTCGCTGG CATCTTGGAC 
CGCAATGATG AGCAATGCCA TCTGAATGTA GATGGAAACT ACACGCATGA TGTTCGTCAA 
GATTACCCAT CTATACCGAC TCTAGTGCGA CTTTTGGTGA AACATAACAT CATCCCAATT 
TTCGCTGTTA CTAATCACTC ATACTCATAC TATGAGAAAC TTCATGAATA CTTCCCCATT 
GCTGAGCTGG GACAGTTACA GGAGGATTCA TCTAATATCC TGAGCATTTT AGAAAAAGCT 
TTTGAGAATA TTCGTTCCAA GATCAGTATT CGTGCAGAGG ACAGACCAAA GGCAGTGGAA 
ACCAAGATTT TCTCTCAGTC TGGCACCTTT TCTGAATATG GCAATTTCAA AATCACACCA 
GGACAAACTG GTAAATTTAA AGTGCGTATG AAGGCCCTTA ATCAGGTAGG AGAAGAGCCC 
GTGTGTAAGG TCAACCAGGC TGACCGCGCA GGAACCTTGA GAGTCAAGCC CACCACCTTC 
AGCTCTGCCT TTAAAATCAA TGCAGAGGTG CTCTGTCCAA CCTGTGACTG TGAAAAGACC 
CCGGTTAAAA ATGCAGTGCG CTGTACTGGC CATGGAGATC TGGTCTGTGG GAAATGCCAG 
TGCTATGCAG GATGGCTCGG CCCTTTGTGT AACTGTTCAG CGGGCGCCTC CACAGACTCT 
GTCATGTGCA TAGAACGAGG AAAGACAGAG CCGTGCTCAG GCAGAGGAGA CTGCATGTGT 
GGGACCTGTG TGTGTTATAA CCCCAACCAG TTTGAAGGGC CCTACTGCCA GTTTGACAAA 
TCTCAGTGCC AGAGATTTGG TGGATTTCTC TGTAATGAGC GTGGAAGCTG CAGCATGGGC 
AGATGTGTTT GTTCTCCGGG ATGGAGTGGA GAGGCCTGTG AATGTCCCAC CAGCAATGAC 
TCATGCCGGG ATAGCAAAGG GGGCATCTGT AATGACCGTG GTGTGTGTAA ATGTGGCCGC 
TGTGAGTGTG AACACTCAGG ACTTCCACTG AGTCCTACCT GCGAAGCCAA CTTTCAGCAA 
CAAGTGGGTA TGTGTGAAGC CAAGAGGAGC TGCGTCCAGT GCCAGGCCTG GAGCACCGGT 
GAAAGGAAAG GACGGAAATG CGATGAGTGT CCCTTCAAAA TTGTCAGGGT GGATGAGCTT 
GAAGAAGATA AAAATGTAAT CGAGACATGC AGTTTCCGTG ATGAAGATGA TGACTGCACA 
TATCACTATA ATGTGAATTA TCCATCGAAC ATCACAGACA AAGAACACCG CGTCCTAGTG 
AAGAAAAAGA AAGATTGTCC ACCTGCAGGT TTCCTGTGGC TCATTCCTCT CATTATGTTT 
CTGATGCTGT TACTTGGTTT GCTTTTGCTC TGTTGCTGGA AATACTGCGC CTGCTGCCAG 
TCCTGTCTTG CTCTTCTCCC GTGCTGTGCA AGAGGTCGTA TGGTGGGCTT TAAAGAAAAC 
CAGTACCTGC TACGTCAGTC TTTTCTGCAA AATGATTATT TGGACACCCC AATGGTGCGC 
AGCGGCCCGC TCAAGAGCAC AGACGTTGTG CGCTGGAAAG TGGCAGACAA TGTTCATCGA 
GGAACGAATC ACCCCCAGAA CCAAATCAGA CCCAACCCCA AAGAGACCAT CCAGTATCCT 
GTTTCTCTTC GTCTGAACCG CCAGTTTTCA GAAGTTCTTT CTAACCCTGA CGCTCGGGAC 
ACAGACATGC TCAGACGAGA AGTAGAGGAC AACCTCAATG ACGTGTTCCT GCAGATCCCG 
GGCGCACAAC CAGTGCAGAA GACTCTGTTC AGGACACAAA GAAATTCTGG AAAGAGACAG 
AACAACACAA TTGTGGACAC AGTTTTATCT GCCCCTCGCT CCAGTTACCC AGATATTGTA 
AAACTGACAG ATAAACAGGT TCAGTCTGGA AACTTCCGGG ACCTGAAGGT GATTCCAGGC 
TACTACACTG TGGCTTCTGA TAAAGAAGCA GCAGGCGCAG TGGAGTTTCC GGAGAACGTG 
GAATCAGTGG ATGTTCGGGT TCCTCTCTTC ATTAAAGATG AGGACGATGA AAAGAAGGAG 
CTGCTTGTGC AAGCTGTGGA TGTTCCAGTC GGAATCGCAA AGATTGGAAA AGATCATGTC 
AACATTACAG TGCTTAAGGA ACAGGCTTCG AGTGTCTTTA CCTTCCTGGA GCCGTCATAT 
ACCTACAGCC GTCAAGATGG AGTGGCCAAC ATCCCGATCA AACGTCAGAT CATAGAGGAT 
GGACGGGCAC AAGTCACATA CAGCACAGCA GACCTCACTG CTAAAGACAA GAAGGACTAT 
ATTTCGGTAG ATGGAGACTT GAGCTATGGA GCAGGTGAAA CAGAGAAGAT TGTCCCAGTG 
AAACTTCTGG AACTTGGAGA GCAAGATGGC CTGCTGGAGG ACAAGCAGGT CAAACAGTTT 
GTCATGGATT TGACCAACCC ACGACAAAGT GCTAAACTAG GCCGTTACCC ACGCACTGTT 
ATCACCATCG CTGACAAACC AGAATCCAGT GTTATGGCGT TTAAGAAGAG TACTCAAACT 
TTCAGTACCA CCGACTCGCT CTACACCATC CCGGTGGAAC GCACTGGAAA CCGGGAGACT 
CCGGCCACTG TGCACTGGCG CACCAACAAG GCCTCGCGCT TCGACATCTC CAGCCCTCTC 
AAATTTGCAC CCGGTGAAGC TGAAAAAAAT ATCCTCATCA ATCCTCGTAC ACATCCATCT 
CCCATCATAC CTGAGAAATT TAACCTGGAA CTATTAGACC CAAGCAACAA TGCAATTATC 
GGAAAGAGGA AGACCACGGA GGTGATCGTC ACCGACGGTC GAGGGGATGG TAAAAATCAG 
GATTTCCAGA AGATGGAATA TGTTACTCAA ACAGCCACAT CCCCCGGAGG ACGCCTCTAT 
TCTCCAGCCA ACATTAAAGC CGTTGCTACC GGCCCTAAAA ACATCCGCCT GAACTGGAAA 
CCCAGCCAAA ATGCCAATGG TTACAAGGTG AAGTACTGGA TTTATGGAGA CCCCGAAGAT 
AAAGCTAAAT TTATGGATGT GAAGACGCCT CAAGCCGAGC TGACAAATCT CGATCCATAC 
TGTGATTACG AGATGCGTGT TTGTGGCTAC AATGCACTTG GAGATGGCAA TTACAGTGAT 
ATCATCCAAT GCCAAACCCT GGAGGATATA CCTGGTGAAC CTGGTCGTCT GGCTTTCAAC 
GTCATCAGCC CTACTGTTAC TCAGGTTAGT TGGGCGGAGC CAGCAGAAGC CAATGGTATC 
ATCACTGGCT ATGAGGTCAT CTACATGCCC ATTAACGAGG ACAAGAAGCC TACAGGACCT 
CCTAAGAAGG TGATGATTGA CAACCCTAAA AAGCGCATGT TGCTCATTGA GAACCTGCAA 
CCTTCTCAGA CCTACTGTTA TAAAGTGCGT GCCAGCAATA AGGTGGGCTG GGGCCCATAC 
AGAGAAGCCA CAATCAACCT GGCATCACAG CCTACCAGAC CCATGTCAAT TCCCATCATT 
CCCGACATTC CCATCGTGGA TGCAGAAGCT GGCGAAGAGT ACGACAGCTA TCTGATGTAC 
AGTAATGAAG TCCTGCGATC CCCAACGAGC AGCCTGAGGC CTAGCGTCAC AGATCTGTCT 
GATGATCAGT TCGTGAACGG AAAGTGGGAA CAGAATTTCT TGTTTCCTGG AGGAGGAGGT 
GGTTCCCTCT CCCGCAACAT CAGCTCTTCC TCAACCACAT ACAGCCATTC CACACCGCGT 
ACCAATGTGA CCAACAACAG CTCCACCACA ACGTACATCT CTGGCAAAGG AGGCTCAACT 
ACACACAGAT ATGATGTAAG AGACGGTGGA CTTCTCAAAG AAGACGTGAT CCTGAGAAAA 
CGCTCTGAAA ACAGGCATTA CACAGAAGGT GATGGTGTGA GGGACTCAAT CGTAATGGGA 
AACCTGTCTG GTGGACTTTT GGACAATTTA GGGGTCTCTA GCTTCAGCCA GACTACATCT 
ACCAGCTACA GCTTGAGCTC CAGAGCTCGT ACTCAGTCTG AAGATATCAA CGATGCTCTG 
CTGAACCTGG ATAGTGTTCT TCAGGAATCG CGCTTCACCC CGGGTGTTCC CGCGACTCCC 
AGCAGACTGG TGTTTTCAGC TCTGGGCCAC ACGGCTCTCA AAGTGAGCTG GCAGGAGCCA 
CACTGCGAGT CTGATGTACT GGGATACTGC GTCCTTTACC AGCTCATCAA TGGAGGTGAC 
GTAAAACGTA TCGAGGTGAA CAATCCTGCT CAGAACTCAG TGCTGGTTCA GGATCTCCTG 
CCTAACCAGT CCTACATCTT CAAAGTCAAA GCACAGAGTC GAGAGGGCTG GGGTCCTGAG 
AGAGAGGGCG TCATTACCAT TGAGTCCAAC GTCGACCCCA AGAGTCCACT GAGCCCAGTG 
CCCGGGTCTC CGTTCACTCT CAGCACTCCC AGCGCTCCAG GACCTCTGAT CTTCACAGCG 
TTACGTCCAG ATACACTGCA GCTGAGCTGG GAGAAACCCC GAAAACCCAA CGGAGACATC 
CTGGGATACG TGGTGACCTG CGAACAGCTG CATGGTGGAG GAGATCTTCG CTCCTTCCAG 
GTGAATGGCA ATGCAGCCAC TTCATTGACT GTCTCTGACC TGAAAGAAAA CATGCCTTAC 
AAATTCAAAG TTCAAGCTCA GACCACGCAG GGCTTTGGCC CAGAGCGAGA GGGAATCATC 
ACCATCGAGT CCCAGGATGG AGGCTCCATG TCCCAATACA GCAGTCAGTC GGTCACGAAG 
AGAGAGGTGT TCAATCTGCC CACAGAAATC ACACACACTT CACACACCAT GTTCTCAGAG 
CCCTTTGTTT CTTCAGACGG GATGATGATG ATGTCGAGCA GGCAGATCAC AGAGTCGGGT 
GGCTCCATGA CACGGCAGGT GGAGATGGTG CAGAGAGGAG TGATGTCCAG CAGCACCGTC 
ACCAAGAGGC TGGAGAGACA GTACTACGAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166847.1
CDS	470..6265
Translation	MGGWAIRLAVGIFLLLLAATDSRADEVNHCTAARTKTCSECIQVGAGCAYCPDEVFEHERCDLKENLEAQKCSKIVNFESSRQIQENVKINEQLKQSQVSPQLMSMTLLPGEEKEVEMEVFEPTRGPLDLYILMDFSNSMSDDLDNLKRMGEKLARLVGDLSDDYTIGFGKFVDKVTEPQTDMRPAKLAEPWSNSDPPFSFRNVIKLTSNITSFRQKLQKERISGNLDAPEGGFDAILQTAVCQDQIGWRKDSTHLLVFSTESAFHYEADGANVLAGILDRNDEQCHLNVDGNYTHDVRQDYPSIPTLVRLLVKHNIIPIFAVTNHSYSYYEKLHEYFPIAELGQLQEDSSNILSILEKAFENIRSKISIRAEDRPKAVETKIFSQSGTFSEYGNFKITPGQTGKFKVRMKALNQVGEEPVCKVNQADRAGTLRVKPTTFSSAFKINAEVLCPTCDCEKTPVKNAVRCTGHGDLVCGKCQCYAGWLGPLCNCSAGASTDSVMCIERGKTEPCSGRGDCMCGTCVCYNPNQFEGPYCQFDKSQCQRFGGFLCNERGSCSMGRCVCSPGWSGEACECPTSNDSCRDSKGGICNDRGVCKCGRCECEHSGLPLSPTCEANFQQQVGMCEAKRSCVQCQAWSTGERKGRKCDECPFKIVRVDELEEDKNVIETCSFRDEDDDCTYHYNVNYPSNITDKEHRVLVKKKKDCPPAGFLWLIPLIMFLMLLLGLLLLCCWKYCACCQSCLALLPCCARGRMVGFKENQYLLRQSFLQNDYLDTPMVRSGPLKSTDVVRWKVADNVHRGTNHPQNQIRPNPKETIQYPVSLRLNRQFSEVLSNPDARDTDMLRREVEDNLNDVFLQIPGAQPVQKTLFRTQRNSGKRQNNTIVDTVLSAPRSSYPDIVKLTDKQVQSGNFRDLKVIPGYYTVASDKEAAGAVEFPENVESVDVRVPLFIKDEDDEKKELLVQAVDVPVGIAKIGKDHVNITVLKEQASSVFTFLEPSYTYSRQDGVANIPIKRQIIEDGRAQVTYSTADLTAKDKKDYISVDGDLSYGAGETEKIVPVKLLELGEQDGLLEDKQVKQFVMDLTNPRQSAKLGRYPRTVITIADKPESSVMAFKKSTQTFSTTDSLYTIPVERTGNRETPATVHWRTNKASRFDISSPLKFAPGEAEKNILINPRTHPSPIIPEKFNLELLDPSNNAIIGKRKTTEVIVTDGRGDGKNQDFQKMEYVTQTATSPGGRLYSPANIKAVATGPKNIRLNWKPSQNANGYKVKYWIYGDPEDKAKFMDVKTPQAELTNLDPYCDYEMRVCGYNALGDGNYSDIIQCQTLEDIPGEPGRLAFNVISPTVTQVSWAEPAEANGIITGYEVIYMPINEDKKPTGPPKKVMIDNPKKRMLLIENLQPSQTYCYKVRASNKVGWGPYREATINLASQPTRPMSIPIIPDIPIVDAEAGEEYDSYLMYSNEVLRSPTSSLRPSVTDLSDDQFVNGKWEQNFLFPGGGGGSLSRNISSSSTTYSHSTPRTNVTNNSSTTTYISGKGGSTTHRYDVRDGGLLKEDVILRKRSENRHYTEGDGVRDSIVMGNLSGGLLDNLGVSSFSQTTSTSYSLSSRARTQSEDINDALLNLDSVLQESRFTPGVPATPSRLVFSALGHTALKVSWQEPHCESDVLGYCVLYQLINGGDVKRIEVNNPAQNSVLVQDLLPNQSYIFKVKAQSREGWGPEREGVITIESNVDPKSPLSPVPGSPFTLSTPSAPGPLIFTALRPDTLQLSWEKPRKPNGDILGYVVTCEQLHGGGDLRSFQVNGNAATSLTVSDLKENMPYKFKVQAQTTQGFGPEREGIITIESQDGGSMSQYSSQSVTKREVFNLPTEITHTSHTMFSEPFVSSDGMMMMSSRQITESGGSMTRQVEMVQRGVMSSSTVTKRLERQYYEA

Target ORF information:

RefSeq Version	XM_005166790.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio integrin, beta 4 (itgb4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166790.4

ORF Insert Sequence:

ATGGGAGGAT GGGCGATACG TCTGGCTGTG GGGATCTTCC TTCTCTTGCT TGCTGCGACA 
GACAGTCGTG CTGATGAAGT GAATCACTGC ACTGCTGCCA GGACCAAAAC CTGCTCAGAA 
TGTATTCAGG TTGGCGCAGG ATGTGCGTAC TGCCCTGATG AGGTCTTCGA GCATGAGCGT 
TGTGACCTGA AAGAAAACCT GGAGGCCCAA AAATGTAGCA AAATAGTAAA TTTTGAGAGC 
AGCAGGCAAA TCCAAGAGAA TGTGAAAATC AACGAGCAAC TGAAGCAGTC TCAGGTGTCC 
CCTCAGCTCA TGAGCATGAC GCTTCTGCCC GGGGAGGAGA AAGAGGTGGA GATGGAGGTG 
TTTGAACCCA CCCGTGGCCC ACTGGATCTC TACATCCTCA TGGACTTCTC CAACTCCATG 
TCTGACGATC TGGACAACCT TAAGAGGATG GGAGAAAAGC TGGCACGGCT TGTTGGCGAT 
CTCTCTGACG ATTACACCAT CGGATTTGGC AAGTTTGTGG ACAAAGTCAC CGAGCCTCAG 
ACAGACATGA GGCCAGCCAA ATTAGCTGAG CCGTGGTCCA ATAGCGACCC TCCATTTTCC 
TTCCGTAACG TCATCAAGCT CACCAGCAAC ATCACCTCCT TCAGACAGAA GCTACAGAAA 
GAGCGTATAT CTGGCAACCT TGATGCTCCA GAAGGTGGTT TTGATGCCAT CTTACAGACT 
GCTGTCTGCC AGGACCAAAT TGGCTGGAGG AAAGACAGCA CACACTTGCT GGTCTTCTCC 
ACTGAATCAG CTTTCCATTA TGAGGCAGAT GGTGCCAACG TGCTCGCTGG CATCTTGGAC 
CGCAATGATG AGCAATGCCA TCTGAATGTA GATGGAAACT ACACGCATGA TGTTCGTCAA 
GATTACCCAT CTATACCGAC TCTAGTGCGA CTTTTGGTGA AACATAACAT CATCCCAATT 
TTCGCTGTTA CTAATCACTC ATACTCATAC TATGAGAAAC TTCATGAATA CTTCCCCATT 
GCTGAGCTGG GACAGTTACA GGAGGATTCA TCTAATATCC TGAGCATTTT AGAAAAAGCT 
TTTGAGAATA TTCGTTCCAA GATCAGTATT CGTGCAGAGG ACAGACCAAA GGCAGTGGAA 
ACCAAGATTT TCTCTCAGTC TGGCACCTTT TCTGAATATG GCAATTTCAA AATCACACCA 
GGACAAACTG GTAAATTTAA AGTGCGTATG AAGGCCCTTA ATCAGGTAGG AGAAGAGCCC 
GTGTGTAAGG TCAACCAGGC TGACCGCGCA GGAACCTTGA GAGTCAAGCC CACCACCTTC 
AGCTCTGCCT TTAAAATCAA TGCAGAGGTG CTCTGTCCAA CCTGTGACTG TGAAAAGACC 
CCGGTTAAAA ATGCAGTGCG CTGTACTGGC CATGGAGATC TGGTCTGTGG GAAATGCCAG 
TGCTATGCAG GATGGCTCGG CCCTTTGTGT AACTGTTCAG CGGGCGCCTC CACAGACTCT 
GTCATGTGCA TAGAACGAGG AAAGACAGAG CCGTGCTCAG GCAGAGGAGA CTGCATGTGT 
GGGACCTGTG TGTGTTATAA CCCCAACCAG TTTGAAGGGC CCTACTGCCA GTTTGACAAA 
TCTCAGTGCC AGAGATTTGG TGGATTTCTC TGTAATGAGC GTGGAAGCTG CAGCATGGGC 
AGATGTGTTT GTTCTCCGGG ATGGAGTGGA GAGGCCTGTG AATGTCCCAC CAGCAATGAC 
TCATGCCGGG ATAGCAAAGG GGGCATCTGT AATGACCGTG GTGTGTGTAA ATGTGGCCGC 
TGTGAGTGTG AACACTCAGG ACTTCCACTG AGTCCTACCT GCGAAGCCAA CTTTCAGCAA 
CAAGTGGGTA TGTGTGAAGC CAAGAGGAGC TGCGTCCAGT GCCAGGCCTG GAGCACCGGT 
GAAAGGAAAG GACGGAAATG CGATGAGTGT CCCTTCAAAA TTGTCAGGGT GGATGAGCTT 
GAAGAAGATA AAAATGTAAT CGAGACATGC AGTTTCCGTG ATGAAGATGA TGACTGCACA 
TATCACTATA ATGTGAATTA TCCATCGAAC ATCACAGACA AAGAACACCG CGTCCTAGTG 
AAGAAAAAGA AAGATTGTCC ACCTGCAGGT TTCCTGTGGC TCATTCCTCT CATTATGTTT 
CTGATGCTGT TACTTGGTTT GCTTTTGCTC TGTTGCTGGA AATACTGCGC CTGCTGCCAG 
TCCTGTCTTG CTCTTCTCCC GTGCTGTGCA AGAGGTCGTA TGGTGGGCTT TAAAGAAAAC 
CAGTACCTGC TACGTCAGTC TTTTCTGCAA AATGATTATT TGGACACCCC AATGGTGCGC 
AGCGGCCCGC TCAAGAGCAC AGACGTTGTG CGCTGGAAAG TGGCAGACAA TGTTCATCGA 
GGAACGAATC ACCCCCAGAA CCAAATCAGA CCCAACCCCA AAGAGACCAT CCAGTATCCT 
GTTTCTCTTC GTCTGAACCG CCAGTTTTCA GAAGTTCTTT CTAACCCTGA CGCTCGGGAC 
ACAGACATGC TCAGACGAGA AGTAGAGGAC AACCTCAATG ACGTGTTCCT GCAGATCCCG 
GGCGCACAAC CAGTGCAGAA GACTCTGTTC AGGACACAAA GAAATTCTGG AAAGAGACAG 
AACAACACAA TTGTGGACAC AGTTTTATCT GCCCCTCGCT CCAGTTACCC AGATATTGTA 
AAACTGACAG ATAAACAGGT TCAGTCTGGA AACTTCCGGG ACCTGAAGGT GATTCCAGGC 
TACTACACTG TGGCTTCTGA TAAAGAAGCA GCAGGCGCAG TGGAGTTTCC GGAGAACGTG 
GAATCAGTGG ATGTTCGGGT TCCTCTCTTC ATTAAAGATG AGGACGATGA AAAGAAGGAG 
CTGCTTGTGC AAGCTGTGGA TGTTCCAGTC GGAATCGCAA AGATTGGAAA AGATCATGTC 
AACATTACAG TGCTTAAGGA ACAGGCTTCG AGTGTCTTTA CCTTCCTGGA GCCGTCATAT 
ACCTACAGCC GTCAAGATGG AGTGGCCAAC ATCCCGATCA AACGTCAGAT CATAGAGGAT 
GGACGGGCAC AAGTCACATA CAGCACAGCA GACCTCACTG CTAAAGACAA GAAGGACTAT 
ATTTCGGTAG ATGGAGACTT GAGCTATGGA GCAGGTGAAA CAGAGAAGAT TGTCCCAGTG 
AAACTTCTGG AACTTGGAGA GCAAGATGGC CTGCTGGAGG ACAAGCAGGT CAAACAGTTT 
GTCATGGATT TGACCAACCC ACGACAAAGT GCTAAACTAG GCCGTTACCC ACGCACTGTT 
ATCACCATCG CTGACAAACC AGAATCCAGT GTTATGGCGT TTAAGAAGAG TACTCAAACT 
TTCAGTACCA CCGACTCGCT CTACACCATC CCGGTGGAAC GCACTGGAAA CCGGGAGACT 
CCGGCCACTG TGCACTGGCG CACCAACAAG GCCTCGCGCT TCGACATCTC CAGCCCTCTC 
AAATTTGCAC CCGGTGAAGC TGAAAAAAAT ATCCTCATCA ATCCTCGTAC ACATCCATCT 
CCCATCATAC CTGAGAAATT TAACCTGGAA CTATTAGACC CAAGCAACAA TGCAATTATC 
GGAAAGAGGA AGACCACGGA GGTGATCGTC ACCGACGGTC GAGGGGATGG TAAAAATCAG 
GATTTCCAGA AGATGGAATA TGTTACTCAA ACAGCCACAT CCCCCGGAGG ACGCCTCTAT 
TCTCCAGCCA ACATTAAAGC CGTTGCTACC GGCCCTAAAA ACATCCGCCT GAACTGGAAA 
CCCAGCCAAA ATGCCAATGG TTACAAGGTG AAGTACTGGA TTTATGGAGA CCCCGAAGAT 
AAAGCTAAAT TTATGGATGT GAAGACGCCT CAAGCCGAGC TGACAAATCT CGATCCATAC 
TGTGATTACG AGATGCGTGT TTGTGGCTAC AATGCACTTG GAGATGGCAA TTACAGTGAT 
ATCATCCAAT GCCAAACCCT GGAGGATATA CCTGGTGAAC CTGGTCGTCT GGCTTTCAAC 
GTCATCAGCC CTACTGTTAC TCAGGTTAGT TGGGCGGAGC CAGCAGAAGC CAATGGTATC 
ATCACTGGCT ATGAGGTCAT CTACATGCCC ATTAACGAGG ACAAGAAGCC TACAGGACCT 
CCTAAGAAGG TGATGATTGA CAACCCTAAA AAGCGCATGT TGCTCATTGA GAACCTGCAA 
CCTTCTCAGA CCTACTGTTA TAAAGTGCGT GCCAGCAATA AGGTGGGCTG GGGCCCATAC 
AGAGAAGCCA CAATCAACCT GGCATCACAG CCTACCAGAC CCATGTCAAT TCCCATCATT 
CCCGACATTC CCATCGTGGA TGCAGAAGCT GGCGAAGAGT ACGACAGCTA TCTGATGTAC 
AGTAATGAAG TCCTGCGATC CCCAACGAGC AGCCTGAGGC CTAGCGTCAC AGATCTGTCT 
GATGATCAGT TCGTGAACGG AAAGTGGGAA CAGAATTTCT TGTTTCCTGG AGGAGGAGGT 
GGTTCCCTCT CCCGCAACAT CAGCTCTTCC TCAACCACAT ACAGCCATTC CACACCGCGT 
ACCAATGTGA CCAACAACAG CTCCACCACA ACGTACATCT CTGGCAAAGG AGGCTCAACT 
ACACACAGAT ATGATGTAAG AGACGGTGGA CTTCTCAAAG AAGACGTGAT CCTGAGAAAA 
CGCTCTGAAA ACAGGCATTA CACAGAAGGT GATGGTGTGA GGGACTCAAT CGTAATGGGA 
AACCTGTCTG GTGGACTTTT GGACAATTTA GGGGTCTCTA GCTTCAGCCA GACTACATCT 
ACCAGCTACA GCTTGAGCTC CAGAGCTCGT ACTCAGTCTG AAGATATCAA CGATGCTCTG 
CTGAACCTGG ATAGTGTTCT TCAGGAATCG CGCTTCACCC CGGGTGTTCC CGCGACTCCC 
AGCAGACTGG TGTTTTCAGC TCTGGGCCAC ACGGCTCTCA AAGTGAGCTG GCAGGAGCCA 
CACTGCGAGT CTGATGTACT GGGATACTGC GTCCTTTACC AGCTCATCAA TGGAGGTGAC 
GTAAAACGTA TCGAGGTGAA CAATCCTGCT CAGAACTCAG TGCTGGTTCA GGATCTCCTG 
CCTAACCAGT CCTACATCTT CAAAGTCAAA GCACAGAGTC GAGAGGGCTG GGGTCCTGAG 
AGAGAGGGCG TCATTACCAT TGAGTCCAAC GTCGACCCCA AGAGTCCACT GAGCCCAGTG 
CCCGGGTCTC CGTTCACTCT CAGCACTCCC AGCGCTCCAG GACCTCTGAT CTTCACAGCG 
TTACGTCCAG ATACACTGCA GCTGAGCTGG GAGAAACCCC GAAAACCCAA CGGAGACATC 
CTGGGATACG TGGTGACCTG CGAACAGCTG CATGGTGGAG GAGATCTTCG CTCCTTCCAG 
GTGAATGGCA ATGCAGCCAC TTCATTGACT GTCTCTGACC TGAAAGAAAA CATGCCTTAC 
AAATTCAAAG TTCAAGCTCA GACCACGCAG GGCTTTGGCC CAGAGCGAGA GGGAATCATC 
ACCATCGAGT CCCAGGATGG AGGCTCCATG TCCCAATACA GCAGTCAGTC GGTCACGAAG 
AGAGAGGTGT TCAATCTGCC CACAGAAATC ACACACACTT CACACACCAT GTTCTCAGAG 
CCCTTTGTTT CTTCAGACGG GATGATGATG ATGTCGAGCA GGCAGATCAC AGAGTCGGGT 
GGCTCCATGA CACGGCAGGT GGAGATGGTG CAGAGAGGAG TGATGTCCAG CAGCACCGTC 
ACCAAGAGGC TGGAGAGACA GTACTACGAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itgb4 ( XM_005166791.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166848.1 Danio rerio integrin, beta 4 (itgb4), transcript variant X2, mRNA.

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CloneID	ODa13938
Clone ID Related Accession (Same CDS sequence)	XM_005166790.4 , XM_005166791.3 , XM_005166790.3 , XM_005166791.4
Accession Version	XM_005166791.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	integrin beta-4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166791.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGGAT GGGCGATACG TCTGGCTGTG GGGATCTTCC TTCTCTTGCT TGCTGCGACA 
GACAGTCGTG CTGATGAAGT GAATCACTGC ACTGCTGCCA GGACCAAAAC CTGCTCAGAA 
TGTATTCAGG TTGGCGCAGG ATGTGCGTAC TGCCCTGATG AGGTCTTCGA GCATGAGCGT 
TGTGACCTGA AAGAAAACCT GGAGGCCCAA AAATGTAGCA AAATAGTAAA TTTTGAGAGC 
AGCAGGCAAA TCCAAGAGAA TGTGAAAATC AACGAGCAAC TGAAGCAGTC TCAGGTGTCC 
CCTCAGCTCA TGAGCATGAC GCTTCTGCCC GGGGAGGAGA AAGAGGTGGA GATGGAGGTG 
TTTGAACCCA CCCGTGGCCC ACTGGATCTC TACATCCTCA TGGACTTCTC CAACTCCATG 
TCTGACGATC TGGACAACCT TAAGAGGATG GGAGAAAAGC TGGCACGGCT TGTTGGCGAT 
CTCTCTGACG ATTACACCAT CGGATTTGGC AAGTTTGTGG ACAAAGTCAC CGAGCCTCAG 
ACAGACATGA GGCCAGCCAA ATTAGCTGAG CCGTGGTCCA ATAGCGACCC TCCATTTTCC 
TTCCGTAACG TCATCAAGCT CACCAGCAAC ATCACCTCCT TCAGACAGAA GCTACAGAAA 
GAGCGTATAT CTGGCAACCT TGATGCTCCA GAAGGTGGTT TTGATGCCAT CTTACAGACT 
GCTGTCTGCC AGGACCAAAT TGGCTGGAGG AAAGACAGCA CACACTTGCT GGTCTTCTCC 
ACTGAATCAG CTTTCCATTA TGAGGCAGAT GGTGCCAACG TGCTCGCTGG CATCTTGGAC 
CGCAATGATG AGCAATGCCA TCTGAATGTA GATGGAAACT ACACGCATGA TGTTCGTCAA 
GATTACCCAT CTATACCGAC TCTAGTGCGA CTTTTGGTGA AACATAACAT CATCCCAATT 
TTCGCTGTTA CTAATCACTC ATACTCATAC TATGAGAAAC TTCATGAATA CTTCCCCATT 
GCTGAGCTGG GACAGTTACA GGAGGATTCA TCTAATATCC TGAGCATTTT AGAAAAAGCT 
TTTGAGAATA TTCGTTCCAA GATCAGTATT CGTGCAGAGG ACAGACCAAA GGCAGTGGAA 
ACCAAGATTT TCTCTCAGTC TGGCACCTTT TCTGAATATG GCAATTTCAA AATCACACCA 
GGACAAACTG GTAAATTTAA AGTGCGTATG AAGGCCCTTA ATCAGGTAGG AGAAGAGCCC 
GTGTGTAAGG TCAACCAGGC TGACCGCGCA GGAACCTTGA GAGTCAAGCC CACCACCTTC 
AGCTCTGCCT TTAAAATCAA TGCAGAGGTG CTCTGTCCAA CCTGTGACTG TGAAAAGACC 
CCGGTTAAAA ATGCAGTGCG CTGTACTGGC CATGGAGATC TGGTCTGTGG GAAATGCCAG 
TGCTATGCAG GATGGCTCGG CCCTTTGTGT AACTGTTCAG CGGGCGCCTC CACAGACTCT 
GTCATGTGCA TAGAACGAGG AAAGACAGAG CCGTGCTCAG GCAGAGGAGA CTGCATGTGT 
GGGACCTGTG TGTGTTATAA CCCCAACCAG TTTGAAGGGC CCTACTGCCA GTTTGACAAA 
TCTCAGTGCC AGAGATTTGG TGGATTTCTC TGTAATGAGC GTGGAAGCTG CAGCATGGGC 
AGATGTGTTT GTTCTCCGGG ATGGAGTGGA GAGGCCTGTG AATGTCCCAC CAGCAATGAC 
TCATGCCGGG ATAGCAAAGG GGGCATCTGT AATGACCGTG GTGTGTGTAA ATGTGGCCGC 
TGTGAGTGTG AACACTCAGG ACTTCCACTG AGTCCTACCT GCGAAGCCAA CTTTCAGCAA 
CAAGTGGGTA TGTGTGAAGC CAAGAGGAGC TGCGTCCAGT GCCAGGCCTG GAGCACCGGT 
GAAAGGAAAG GACGGAAATG CGATGAGTGT CCCTTCAAAA TTGTCAGGGT GGATGAGCTT 
GAAGAAGATA AAAATGTAAT CGAGACATGC AGTTTCCGTG ATGAAGATGA TGACTGCACA 
TATCACTATA ATGTGAATTA TCCATCGAAC ATCACAGACA AAGAACACCG CGTCCTAGTG 
AAGAAAAAGA AAGATTGTCC ACCTGCAGGT TTCCTGTGGC TCATTCCTCT CATTATGTTT 
CTGATGCTGT TACTTGGTTT GCTTTTGCTC TGTTGCTGGA AATACTGCGC CTGCTGCCAG 
TCCTGTCTTG CTCTTCTCCC GTGCTGTGCA AGAGGTCGTA TGGTGGGCTT TAAAGAAAAC 
CAGTACCTGC TACGTCAGTC TTTTCTGCAA AATGATTATT TGGACACCCC AATGGTGCGC 
AGCGGCCCGC TCAAGAGCAC AGACGTTGTG CGCTGGAAAG TGGCAGACAA TGTTCATCGA 
GGAACGAATC ACCCCCAGAA CCAAATCAGA CCCAACCCCA AAGAGACCAT CCAGTATCCT 
GTTTCTCTTC GTCTGAACCG CCAGTTTTCA GAAGTTCTTT CTAACCCTGA CGCTCGGGAC 
ACAGACATGC TCAGACGAGA AGTAGAGGAC AACCTCAATG ACGTGTTCCT GCAGATCCCG 
GGCGCACAAC CAGTGCAGAA GACTCTGTTC AGGACACAAA GAAATTCTGG AAAGAGACAG 
AACAACACAA TTGTGGACAC AGTTTTATCT GCCCCTCGCT CCAGTTACCC AGATATTGTA 
AAACTGACAG ATAAACAGGT TCAGTCTGGA AACTTCCGGG ACCTGAAGGT GATTCCAGGC 
TACTACACTG TGGCTTCTGA TAAAGAAGCA GCAGGCGCAG TGGAGTTTCC GGAGAACGTG 
GAATCAGTGG ATGTTCGGGT TCCTCTCTTC ATTAAAGATG AGGACGATGA AAAGAAGGAG 
CTGCTTGTGC AAGCTGTGGA TGTTCCAGTC GGAATCGCAA AGATTGGAAA AGATCATGTC 
AACATTACAG TGCTTAAGGA ACAGGCTTCG AGTGTCTTTA CCTTCCTGGA GCCGTCATAT 
ACCTACAGCC GTCAAGATGG AGTGGCCAAC ATCCCGATCA AACGTCAGAT CATAGAGGAT 
GGACGGGCAC AAGTCACATA CAGCACAGCA GACCTCACTG CTAAAGACAA GAAGGACTAT 
ATTTCGGTAG ATGGAGACTT GAGCTATGGA GCAGGTGAAA CAGAGAAGAT TGTCCCAGTG 
AAACTTCTGG AACTTGGAGA GCAAGATGGC CTGCTGGAGG ACAAGCAGGT CAAACAGTTT 
GTCATGGATT TGACCAACCC ACGACAAAGT GCTAAACTAG GCCGTTACCC ACGCACTGTT 
ATCACCATCG CTGACAAACC AGAATCCAGT GTTATGGCGT TTAAGAAGAG TACTCAAACT 
TTCAGTACCA CCGACTCGCT CTACACCATC CCGGTGGAAC GCACTGGAAA CCGGGAGACT 
CCGGCCACTG TGCACTGGCG CACCAACAAG GCCTCGCGCT TCGACATCTC CAGCCCTCTC 
AAATTTGCAC CCGGTGAAGC TGAAAAAAAT ATCCTCATCA ATCCTCGTAC ACATCCATCT 
CCCATCATAC CTGAGAAATT TAACCTGGAA CTATTAGACC CAAGCAACAA TGCAATTATC 
GGAAAGAGGA AGACCACGGA GGTGATCGTC ACCGACGGTC GAGGGGATGG TAAAAATCAG 
GATTTCCAGA AGATGGAATA TGTTACTCAA ACAGCCACAT CCCCCGGAGG ACGCCTCTAT 
TCTCCAGCCA ACATTAAAGC CGTTGCTACC GGCCCTAAAA ACATCCGCCT GAACTGGAAA 
CCCAGCCAAA ATGCCAATGG TTACAAGGTG AAGTACTGGA TTTATGGAGA CCCCGAAGAT 
AAAGCTAAAT TTATGGATGT GAAGACGCCT CAAGCCGAGC TGACAAATCT CGATCCATAC 
TGTGATTACG AGATGCGTGT TTGTGGCTAC AATGCACTTG GAGATGGCAA TTACAGTGAT 
ATCATCCAAT GCCAAACCCT GGAGGATATA CCTGGTGAAC CTGGTCGTCT GGCTTTCAAC 
GTCATCAGCC CTACTGTTAC TCAGGTTAGT TGGGCGGAGC CAGCAGAAGC CAATGGTATC 
ATCACTGGCT ATGAGGTCAT CTACATGCCC ATTAACGAGG ACAAGAAGCC TACAGGACCT 
CCTAAGAAGG TGATGATTGA CAACCCTAAA AAGCGCATGT TGCTCATTGA GAACCTGCAA 
CCTTCTCAGA CCTACTGTTA TAAAGTGCGT GCCAGCAATA AGGTGGGCTG GGGCCCATAC 
AGAGAAGCCA CAATCAACCT GGCATCACAG CCTACCAGAC CCATGTCAAT TCCCATCATT 
CCCGACATTC CCATCGTGGA TGCAGAAGCT GGCGAAGAGT ACGACAGCTA TCTGATGTAC 
AGTAATGAAG TCCTGCGATC CCCAACGAGC AGCCTGAGGC CTAGCGTCAC AGATCTGTCT 
GATGATCAGT TCGTGAACGG AAAGTGGGAA CAGAATTTCT TGTTTCCTGG AGGAGGAGGT 
GGTTCCCTCT CCCGCAACAT CAGCTCTTCC TCAACCACAT ACAGCCATTC CACACCGCGT 
ACCAATGTGA CCAACAACAG CTCCACCACA ACGTACATCT CTGGCAAAGG AGGCTCAACT 
ACACACAGAT ATGATGTAAG AGACGGTGGA CTTCTCAAAG AAGACGTGAT CCTGAGAAAA 
CGCTCTGAAA ACAGGCATTA CACAGAAGGT GATGGTGTGA GGGACTCAAT CGTAATGGGA 
AACCTGTCTG GTGGACTTTT GGACAATTTA GGGGTCTCTA GCTTCAGCCA GACTACATCT 
ACCAGCTACA GCTTGAGCTC CAGAGCTCGT ACTCAGTCTG AAGATATCAA CGATGCTCTG 
CTGAACCTGG ATAGTGTTCT TCAGGAATCG CGCTTCACCC CGGGTGTTCC CGCGACTCCC 
AGCAGACTGG TGTTTTCAGC TCTGGGCCAC ACGGCTCTCA AAGTGAGCTG GCAGGAGCCA 
CACTGCGAGT CTGATGTACT GGGATACTGC GTCCTTTACC AGCTCATCAA TGGAGGTGAC 
GTAAAACGTA TCGAGGTGAA CAATCCTGCT CAGAACTCAG TGCTGGTTCA GGATCTCCTG 
CCTAACCAGT CCTACATCTT CAAAGTCAAA GCACAGAGTC GAGAGGGCTG GGGTCCTGAG 
AGAGAGGGCG TCATTACCAT TGAGTCCAAC GTCGACCCCA AGAGTCCACT GAGCCCAGTG 
CCCGGGTCTC CGTTCACTCT CAGCACTCCC AGCGCTCCAG GACCTCTGAT CTTCACAGCG 
TTACGTCCAG ATACACTGCA GCTGAGCTGG GAGAAACCCC GAAAACCCAA CGGAGACATC 
CTGGGATACG TGGTGACCTG CGAACAGCTG CATGGTGGAG GAGATCTTCG CTCCTTCCAG 
GTGAATGGCA ATGCAGCCAC TTCATTGACT GTCTCTGACC TGAAAGAAAA CATGCCTTAC 
AAATTCAAAG TTCAAGCTCA GACCACGCAG GGCTTTGGCC CAGAGCGAGA GGGAATCATC 
ACCATCGAGT CCCAGGATGG AGGCTCCATG TCCCAATACA GCAGTCAGTC GGTCACGAAG 
AGAGAGGTGT TCAATCTGCC CACAGAAATC ACACACACTT CACACACCAT GTTCTCAGAG 
CCCTTTGTTT CTTCAGACGG GATGATGATG ATGTCGAGCA GGCAGATCAC AGAGTCGGGT 
GGCTCCATGA CACGGCAGGT GGAGATGGTG CAGAGAGGAG TGATGTCCAG CAGCACCGTC 
ACCAAGAGGC TGGAGAGACA GTACTACGAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166848.1
CDS	177..5972
Translation	MGGWAIRLAVGIFLLLLAATDSRADEVNHCTAARTKTCSECIQVGAGCAYCPDEVFEHERCDLKENLEAQKCSKIVNFESSRQIQENVKINEQLKQSQVSPQLMSMTLLPGEEKEVEMEVFEPTRGPLDLYILMDFSNSMSDDLDNLKRMGEKLARLVGDLSDDYTIGFGKFVDKVTEPQTDMRPAKLAEPWSNSDPPFSFRNVIKLTSNITSFRQKLQKERISGNLDAPEGGFDAILQTAVCQDQIGWRKDSTHLLVFSTESAFHYEADGANVLAGILDRNDEQCHLNVDGNYTHDVRQDYPSIPTLVRLLVKHNIIPIFAVTNHSYSYYEKLHEYFPIAELGQLQEDSSNILSILEKAFENIRSKISIRAEDRPKAVETKIFSQSGTFSEYGNFKITPGQTGKFKVRMKALNQVGEEPVCKVNQADRAGTLRVKPTTFSSAFKINAEVLCPTCDCEKTPVKNAVRCTGHGDLVCGKCQCYAGWLGPLCNCSAGASTDSVMCIERGKTEPCSGRGDCMCGTCVCYNPNQFEGPYCQFDKSQCQRFGGFLCNERGSCSMGRCVCSPGWSGEACECPTSNDSCRDSKGGICNDRGVCKCGRCECEHSGLPLSPTCEANFQQQVGMCEAKRSCVQCQAWSTGERKGRKCDECPFKIVRVDELEEDKNVIETCSFRDEDDDCTYHYNVNYPSNITDKEHRVLVKKKKDCPPAGFLWLIPLIMFLMLLLGLLLLCCWKYCACCQSCLALLPCCARGRMVGFKENQYLLRQSFLQNDYLDTPMVRSGPLKSTDVVRWKVADNVHRGTNHPQNQIRPNPKETIQYPVSLRLNRQFSEVLSNPDARDTDMLRREVEDNLNDVFLQIPGAQPVQKTLFRTQRNSGKRQNNTIVDTVLSAPRSSYPDIVKLTDKQVQSGNFRDLKVIPGYYTVASDKEAAGAVEFPENVESVDVRVPLFIKDEDDEKKELLVQAVDVPVGIAKIGKDHVNITVLKEQASSVFTFLEPSYTYSRQDGVANIPIKRQIIEDGRAQVTYSTADLTAKDKKDYISVDGDLSYGAGETEKIVPVKLLELGEQDGLLEDKQVKQFVMDLTNPRQSAKLGRYPRTVITIADKPESSVMAFKKSTQTFSTTDSLYTIPVERTGNRETPATVHWRTNKASRFDISSPLKFAPGEAEKNILINPRTHPSPIIPEKFNLELLDPSNNAIIGKRKTTEVIVTDGRGDGKNQDFQKMEYVTQTATSPGGRLYSPANIKAVATGPKNIRLNWKPSQNANGYKVKYWIYGDPEDKAKFMDVKTPQAELTNLDPYCDYEMRVCGYNALGDGNYSDIIQCQTLEDIPGEPGRLAFNVISPTVTQVSWAEPAEANGIITGYEVIYMPINEDKKPTGPPKKVMIDNPKKRMLLIENLQPSQTYCYKVRASNKVGWGPYREATINLASQPTRPMSIPIIPDIPIVDAEAGEEYDSYLMYSNEVLRSPTSSLRPSVTDLSDDQFVNGKWEQNFLFPGGGGGSLSRNISSSSTTYSHSTPRTNVTNNSSTTTYISGKGGSTTHRYDVRDGGLLKEDVILRKRSENRHYTEGDGVRDSIVMGNLSGGLLDNLGVSSFSQTTSTSYSLSSRARTQSEDINDALLNLDSVLQESRFTPGVPATPSRLVFSALGHTALKVSWQEPHCESDVLGYCVLYQLINGGDVKRIEVNNPAQNSVLVQDLLPNQSYIFKVKAQSREGWGPEREGVITIESNVDPKSPLSPVPGSPFTLSTPSAPGPLIFTALRPDTLQLSWEKPRKPNGDILGYVVTCEQLHGGGDLRSFQVNGNAATSLTVSDLKENMPYKFKVQAQTTQGFGPEREGIITIESQDGGSMSQYSSQSVTKREVFNLPTEITHTSHTMFSEPFVSSDGMMMMSSRQITESGGSMTRQVEMVQRGVMSSSTVTKRLERQYYEA

Target ORF information:

RefSeq Version	XM_005166791.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio integrin, beta 4 (itgb4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166791.3

ORF Insert Sequence:

ATGGGAGGAT GGGCGATACG TCTGGCTGTG GGGATCTTCC TTCTCTTGCT TGCTGCGACA 
GACAGTCGTG CTGATGAAGT GAATCACTGC ACTGCTGCCA GGACCAAAAC CTGCTCAGAA 
TGTATTCAGG TTGGCGCAGG ATGTGCGTAC TGCCCTGATG AGGTCTTCGA GCATGAGCGT 
TGTGACCTGA AAGAAAACCT GGAGGCCCAA AAATGTAGCA AAATAGTAAA TTTTGAGAGC 
AGCAGGCAAA TCCAAGAGAA TGTGAAAATC AACGAGCAAC TGAAGCAGTC TCAGGTGTCC 
CCTCAGCTCA TGAGCATGAC GCTTCTGCCC GGGGAGGAGA AAGAGGTGGA GATGGAGGTG 
TTTGAACCCA CCCGTGGCCC ACTGGATCTC TACATCCTCA TGGACTTCTC CAACTCCATG 
TCTGACGATC TGGACAACCT TAAGAGGATG GGAGAAAAGC TGGCACGGCT TGTTGGCGAT 
CTCTCTGACG ATTACACCAT CGGATTTGGC AAGTTTGTGG ACAAAGTCAC CGAGCCTCAG 
ACAGACATGA GGCCAGCCAA ATTAGCTGAG CCGTGGTCCA ATAGCGACCC TCCATTTTCC 
TTCCGTAACG TCATCAAGCT CACCAGCAAC ATCACCTCCT TCAGACAGAA GCTACAGAAA 
GAGCGTATAT CTGGCAACCT TGATGCTCCA GAAGGTGGTT TTGATGCCAT CTTACAGACT 
GCTGTCTGCC AGGACCAAAT TGGCTGGAGG AAAGACAGCA CACACTTGCT GGTCTTCTCC 
ACTGAATCAG CTTTCCATTA TGAGGCAGAT GGTGCCAACG TGCTCGCTGG CATCTTGGAC 
CGCAATGATG AGCAATGCCA TCTGAATGTA GATGGAAACT ACACGCATGA TGTTCGTCAA 
GATTACCCAT CTATACCGAC TCTAGTGCGA CTTTTGGTGA AACATAACAT CATCCCAATT 
TTCGCTGTTA CTAATCACTC ATACTCATAC TATGAGAAAC TTCATGAATA CTTCCCCATT 
GCTGAGCTGG GACAGTTACA GGAGGATTCA TCTAATATCC TGAGCATTTT AGAAAAAGCT 
TTTGAGAATA TTCGTTCCAA GATCAGTATT CGTGCAGAGG ACAGACCAAA GGCAGTGGAA 
ACCAAGATTT TCTCTCAGTC TGGCACCTTT TCTGAATATG GCAATTTCAA AATCACACCA 
GGACAAACTG GTAAATTTAA AGTGCGTATG AAGGCCCTTA ATCAGGTAGG AGAAGAGCCC 
GTGTGTAAGG TCAACCAGGC TGACCGCGCA GGAACCTTGA GAGTCAAGCC CACCACCTTC 
AGCTCTGCCT TTAAAATCAA TGCAGAGGTG CTCTGTCCAA CCTGTGACTG TGAAAAGACC 
CCGGTTAAAA ATGCAGTGCG CTGTACTGGC CATGGAGATC TGGTCTGTGG GAAATGCCAG 
TGCTATGCAG GATGGCTCGG CCCTTTGTGT AACTGTTCAG CGGGCGCCTC CACAGACTCT 
GTCATGTGCA TAGAACGAGG AAAGACAGAG CCGTGCTCAG GCAGAGGAGA CTGCATGTGT 
GGGACCTGTG TGTGTTATAA CCCCAACCAG TTTGAAGGGC CCTACTGCCA GTTTGACAAA 
TCTCAGTGCC AGAGATTTGG TGGATTTCTC TGTAATGAGC GTGGAAGCTG CAGCATGGGC 
AGATGTGTTT GTTCTCCGGG ATGGAGTGGA GAGGCCTGTG AATGTCCCAC CAGCAATGAC 
TCATGCCGGG ATAGCAAAGG GGGCATCTGT AATGACCGTG GTGTGTGTAA ATGTGGCCGC 
TGTGAGTGTG AACACTCAGG ACTTCCACTG AGTCCTACCT GCGAAGCCAA CTTTCAGCAA 
CAAGTGGGTA TGTGTGAAGC CAAGAGGAGC TGCGTCCAGT GCCAGGCCTG GAGCACCGGT 
GAAAGGAAAG GACGGAAATG CGATGAGTGT CCCTTCAAAA TTGTCAGGGT GGATGAGCTT 
GAAGAAGATA AAAATGTAAT CGAGACATGC AGTTTCCGTG ATGAAGATGA TGACTGCACA 
TATCACTATA ATGTGAATTA TCCATCGAAC ATCACAGACA AAGAACACCG CGTCCTAGTG 
AAGAAAAAGA AAGATTGTCC ACCTGCAGGT TTCCTGTGGC TCATTCCTCT CATTATGTTT 
CTGATGCTGT TACTTGGTTT GCTTTTGCTC TGTTGCTGGA AATACTGCGC CTGCTGCCAG 
TCCTGTCTTG CTCTTCTCCC GTGCTGTGCA AGAGGTCGTA TGGTGGGCTT TAAAGAAAAC 
CAGTACCTGC TACGTCAGTC TTTTCTGCAA AATGATTATT TGGACACCCC AATGGTGCGC 
AGCGGCCCGC TCAAGAGCAC AGACGTTGTG CGCTGGAAAG TGGCAGACAA TGTTCATCGA 
GGAACGAATC ACCCCCAGAA CCAAATCAGA CCCAACCCCA AAGAGACCAT CCAGTATCCT 
GTTTCTCTTC GTCTGAACCG CCAGTTTTCA GAAGTTCTTT CTAACCCTGA CGCTCGGGAC 
ACAGACATGC TCAGACGAGA AGTAGAGGAC AACCTCAATG ACGTGTTCCT GCAGATCCCG 
GGCGCACAAC CAGTGCAGAA GACTCTGTTC AGGACACAAA GAAATTCTGG AAAGAGACAG 
AACAACACAA TTGTGGACAC AGTTTTATCT GCCCCTCGCT CCAGTTACCC AGATATTGTA 
AAACTGACAG ATAAACAGGT TCAGTCTGGA AACTTCCGGG ACCTGAAGGT GATTCCAGGC 
TACTACACTG TGGCTTCTGA TAAAGAAGCA GCAGGCGCAG TGGAGTTTCC GGAGAACGTG 
GAATCAGTGG ATGTTCGGGT TCCTCTCTTC ATTAAAGATG AGGACGATGA AAAGAAGGAG 
CTGCTTGTGC AAGCTGTGGA TGTTCCAGTC GGAATCGCAA AGATTGGAAA AGATCATGTC 
AACATTACAG TGCTTAAGGA ACAGGCTTCG AGTGTCTTTA CCTTCCTGGA GCCGTCATAT 
ACCTACAGCC GTCAAGATGG AGTGGCCAAC ATCCCGATCA AACGTCAGAT CATAGAGGAT 
GGACGGGCAC AAGTCACATA CAGCACAGCA GACCTCACTG CTAAAGACAA GAAGGACTAT 
ATTTCGGTAG ATGGAGACTT GAGCTATGGA GCAGGTGAAA CAGAGAAGAT TGTCCCAGTG 
AAACTTCTGG AACTTGGAGA GCAAGATGGC CTGCTGGAGG ACAAGCAGGT CAAACAGTTT 
GTCATGGATT TGACCAACCC ACGACAAAGT GCTAAACTAG GCCGTTACCC ACGCACTGTT 
ATCACCATCG CTGACAAACC AGAATCCAGT GTTATGGCGT TTAAGAAGAG TACTCAAACT 
TTCAGTACCA CCGACTCGCT CTACACCATC CCGGTGGAAC GCACTGGAAA CCGGGAGACT 
CCGGCCACTG TGCACTGGCG CACCAACAAG GCCTCGCGCT TCGACATCTC CAGCCCTCTC 
AAATTTGCAC CCGGTGAAGC TGAAAAAAAT ATCCTCATCA ATCCTCGTAC ACATCCATCT 
CCCATCATAC CTGAGAAATT TAACCTGGAA CTATTAGACC CAAGCAACAA TGCAATTATC 
GGAAAGAGGA AGACCACGGA GGTGATCGTC ACCGACGGTC GAGGGGATGG TAAAAATCAG 
GATTTCCAGA AGATGGAATA TGTTACTCAA ACAGCCACAT CCCCCGGAGG ACGCCTCTAT 
TCTCCAGCCA ACATTAAAGC CGTTGCTACC GGCCCTAAAA ACATCCGCCT GAACTGGAAA 
CCCAGCCAAA ATGCCAATGG TTACAAGGTG AAGTACTGGA TTTATGGAGA CCCCGAAGAT 
AAAGCTAAAT TTATGGATGT GAAGACGCCT CAAGCCGAGC TGACAAATCT CGATCCATAC 
TGTGATTACG AGATGCGTGT TTGTGGCTAC AATGCACTTG GAGATGGCAA TTACAGTGAT 
ATCATCCAAT GCCAAACCCT GGAGGATATA CCTGGTGAAC CTGGTCGTCT GGCTTTCAAC 
GTCATCAGCC CTACTGTTAC TCAGGTTAGT TGGGCGGAGC CAGCAGAAGC CAATGGTATC 
ATCACTGGCT ATGAGGTCAT CTACATGCCC ATTAACGAGG ACAAGAAGCC TACAGGACCT 
CCTAAGAAGG TGATGATTGA CAACCCTAAA AAGCGCATGT TGCTCATTGA GAACCTGCAA 
CCTTCTCAGA CCTACTGTTA TAAAGTGCGT GCCAGCAATA AGGTGGGCTG GGGCCCATAC 
AGAGAAGCCA CAATCAACCT GGCATCACAG CCTACCAGAC CCATGTCAAT TCCCATCATT 
CCCGACATTC CCATCGTGGA TGCAGAAGCT GGCGAAGAGT ACGACAGCTA TCTGATGTAC 
AGTAATGAAG TCCTGCGATC CCCAACGAGC AGCCTGAGGC CTAGCGTCAC AGATCTGTCT 
GATGATCAGT TCGTGAACGG AAAGTGGGAA CAGAATTTCT TGTTTCCTGG AGGAGGAGGT 
GGTTCCCTCT CCCGCAACAT CAGCTCTTCC TCAACCACAT ACAGCCATTC CACACCGCGT 
ACCAATGTGA CCAACAACAG CTCCACCACA ACGTACATCT CTGGCAAAGG AGGCTCAACT 
ACACACAGAT ATGATGTAAG AGACGGTGGA CTTCTCAAAG AAGACGTGAT CCTGAGAAAA 
CGCTCTGAAA ACAGGCATTA CACAGAAGGT GATGGTGTGA GGGACTCAAT CGTAATGGGA 
AACCTGTCTG GTGGACTTTT GGACAATTTA GGGGTCTCTA GCTTCAGCCA GACTACATCT 
ACCAGCTACA GCTTGAGCTC CAGAGCTCGT ACTCAGTCTG AAGATATCAA CGATGCTCTG 
CTGAACCTGG ATAGTGTTCT TCAGGAATCG CGCTTCACCC CGGGTGTTCC CGCGACTCCC 
AGCAGACTGG TGTTTTCAGC TCTGGGCCAC ACGGCTCTCA AAGTGAGCTG GCAGGAGCCA 
CACTGCGAGT CTGATGTACT GGGATACTGC GTCCTTTACC AGCTCATCAA TGGAGGTGAC 
GTAAAACGTA TCGAGGTGAA CAATCCTGCT CAGAACTCAG TGCTGGTTCA GGATCTCCTG 
CCTAACCAGT CCTACATCTT CAAAGTCAAA GCACAGAGTC GAGAGGGCTG GGGTCCTGAG 
AGAGAGGGCG TCATTACCAT TGAGTCCAAC GTCGACCCCA AGAGTCCACT GAGCCCAGTG 
CCCGGGTCTC CGTTCACTCT CAGCACTCCC AGCGCTCCAG GACCTCTGAT CTTCACAGCG 
TTACGTCCAG ATACACTGCA GCTGAGCTGG GAGAAACCCC GAAAACCCAA CGGAGACATC 
CTGGGATACG TGGTGACCTG CGAACAGCTG CATGGTGGAG GAGATCTTCG CTCCTTCCAG 
GTGAATGGCA ATGCAGCCAC TTCATTGACT GTCTCTGACC TGAAAGAAAA CATGCCTTAC 
AAATTCAAAG TTCAAGCTCA GACCACGCAG GGCTTTGGCC CAGAGCGAGA GGGAATCATC 
ACCATCGAGT CCCAGGATGG AGGCTCCATG TCCCAATACA GCAGTCAGTC GGTCACGAAG 
AGAGAGGTGT TCAATCTGCC CACAGAAATC ACACACACTT CACACACCAT GTTCTCAGAG 
CCCTTTGTTT CTTCAGACGG GATGATGATG ATGTCGAGCA GGCAGATCAC AGAGTCGGGT 
GGCTCCATGA CACGGCAGGT GGAGATGGTG CAGAGAGGAG TGATGTCCAG CAGCACCGTC 
ACCAAGAGGC TGGAGAGACA GTACTACGAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itgb4 ( XM_005166790.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166847.1 Danio rerio integrin, beta 4 (itgb4), transcript variant X1, mRNA.

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CloneID	ODa13938
Clone ID Related Accession (Same CDS sequence)	XM_005166790.4 , XM_005166791.3 , XM_005166790.3 , XM_005166791.4
Accession Version	XM_005166790.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	integrin beta-4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166790.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGGAT GGGCGATACG TCTGGCTGTG GGGATCTTCC TTCTCTTGCT TGCTGCGACA 
GACAGTCGTG CTGATGAAGT GAATCACTGC ACTGCTGCCA GGACCAAAAC CTGCTCAGAA 
TGTATTCAGG TTGGCGCAGG ATGTGCGTAC TGCCCTGATG AGGTCTTCGA GCATGAGCGT 
TGTGACCTGA AAGAAAACCT GGAGGCCCAA AAATGTAGCA AAATAGTAAA TTTTGAGAGC 
AGCAGGCAAA TCCAAGAGAA TGTGAAAATC AACGAGCAAC TGAAGCAGTC TCAGGTGTCC 
CCTCAGCTCA TGAGCATGAC GCTTCTGCCC GGGGAGGAGA AAGAGGTGGA GATGGAGGTG 
TTTGAACCCA CCCGTGGCCC ACTGGATCTC TACATCCTCA TGGACTTCTC CAACTCCATG 
TCTGACGATC TGGACAACCT TAAGAGGATG GGAGAAAAGC TGGCACGGCT TGTTGGCGAT 
CTCTCTGACG ATTACACCAT CGGATTTGGC AAGTTTGTGG ACAAAGTCAC CGAGCCTCAG 
ACAGACATGA GGCCAGCCAA ATTAGCTGAG CCGTGGTCCA ATAGCGACCC TCCATTTTCC 
TTCCGTAACG TCATCAAGCT CACCAGCAAC ATCACCTCCT TCAGACAGAA GCTACAGAAA 
GAGCGTATAT CTGGCAACCT TGATGCTCCA GAAGGTGGTT TTGATGCCAT CTTACAGACT 
GCTGTCTGCC AGGACCAAAT TGGCTGGAGG AAAGACAGCA CACACTTGCT GGTCTTCTCC 
ACTGAATCAG CTTTCCATTA TGAGGCAGAT GGTGCCAACG TGCTCGCTGG CATCTTGGAC 
CGCAATGATG AGCAATGCCA TCTGAATGTA GATGGAAACT ACACGCATGA TGTTCGTCAA 
GATTACCCAT CTATACCGAC TCTAGTGCGA CTTTTGGTGA AACATAACAT CATCCCAATT 
TTCGCTGTTA CTAATCACTC ATACTCATAC TATGAGAAAC TTCATGAATA CTTCCCCATT 
GCTGAGCTGG GACAGTTACA GGAGGATTCA TCTAATATCC TGAGCATTTT AGAAAAAGCT 
TTTGAGAATA TTCGTTCCAA GATCAGTATT CGTGCAGAGG ACAGACCAAA GGCAGTGGAA 
ACCAAGATTT TCTCTCAGTC TGGCACCTTT TCTGAATATG GCAATTTCAA AATCACACCA 
GGACAAACTG GTAAATTTAA AGTGCGTATG AAGGCCCTTA ATCAGGTAGG AGAAGAGCCC 
GTGTGTAAGG TCAACCAGGC TGACCGCGCA GGAACCTTGA GAGTCAAGCC CACCACCTTC 
AGCTCTGCCT TTAAAATCAA TGCAGAGGTG CTCTGTCCAA CCTGTGACTG TGAAAAGACC 
CCGGTTAAAA ATGCAGTGCG CTGTACTGGC CATGGAGATC TGGTCTGTGG GAAATGCCAG 
TGCTATGCAG GATGGCTCGG CCCTTTGTGT AACTGTTCAG CGGGCGCCTC CACAGACTCT 
GTCATGTGCA TAGAACGAGG AAAGACAGAG CCGTGCTCAG GCAGAGGAGA CTGCATGTGT 
GGGACCTGTG TGTGTTATAA CCCCAACCAG TTTGAAGGGC CCTACTGCCA GTTTGACAAA 
TCTCAGTGCC AGAGATTTGG TGGATTTCTC TGTAATGAGC GTGGAAGCTG CAGCATGGGC 
AGATGTGTTT GTTCTCCGGG ATGGAGTGGA GAGGCCTGTG AATGTCCCAC CAGCAATGAC 
TCATGCCGGG ATAGCAAAGG GGGCATCTGT AATGACCGTG GTGTGTGTAA ATGTGGCCGC 
TGTGAGTGTG AACACTCAGG ACTTCCACTG AGTCCTACCT GCGAAGCCAA CTTTCAGCAA 
CAAGTGGGTA TGTGTGAAGC CAAGAGGAGC TGCGTCCAGT GCCAGGCCTG GAGCACCGGT 
GAAAGGAAAG GACGGAAATG CGATGAGTGT CCCTTCAAAA TTGTCAGGGT GGATGAGCTT 
GAAGAAGATA AAAATGTAAT CGAGACATGC AGTTTCCGTG ATGAAGATGA TGACTGCACA 
TATCACTATA ATGTGAATTA TCCATCGAAC ATCACAGACA AAGAACACCG CGTCCTAGTG 
AAGAAAAAGA AAGATTGTCC ACCTGCAGGT TTCCTGTGGC TCATTCCTCT CATTATGTTT 
CTGATGCTGT TACTTGGTTT GCTTTTGCTC TGTTGCTGGA AATACTGCGC CTGCTGCCAG 
TCCTGTCTTG CTCTTCTCCC GTGCTGTGCA AGAGGTCGTA TGGTGGGCTT TAAAGAAAAC 
CAGTACCTGC TACGTCAGTC TTTTCTGCAA AATGATTATT TGGACACCCC AATGGTGCGC 
AGCGGCCCGC TCAAGAGCAC AGACGTTGTG CGCTGGAAAG TGGCAGACAA TGTTCATCGA 
GGAACGAATC ACCCCCAGAA CCAAATCAGA CCCAACCCCA AAGAGACCAT CCAGTATCCT 
GTTTCTCTTC GTCTGAACCG CCAGTTTTCA GAAGTTCTTT CTAACCCTGA CGCTCGGGAC 
ACAGACATGC TCAGACGAGA AGTAGAGGAC AACCTCAATG ACGTGTTCCT GCAGATCCCG 
GGCGCACAAC CAGTGCAGAA GACTCTGTTC AGGACACAAA GAAATTCTGG AAAGAGACAG 
AACAACACAA TTGTGGACAC AGTTTTATCT GCCCCTCGCT CCAGTTACCC AGATATTGTA 
AAACTGACAG ATAAACAGGT TCAGTCTGGA AACTTCCGGG ACCTGAAGGT GATTCCAGGC 
TACTACACTG TGGCTTCTGA TAAAGAAGCA GCAGGCGCAG TGGAGTTTCC GGAGAACGTG 
GAATCAGTGG ATGTTCGGGT TCCTCTCTTC ATTAAAGATG AGGACGATGA AAAGAAGGAG 
CTGCTTGTGC AAGCTGTGGA TGTTCCAGTC GGAATCGCAA AGATTGGAAA AGATCATGTC 
AACATTACAG TGCTTAAGGA ACAGGCTTCG AGTGTCTTTA CCTTCCTGGA GCCGTCATAT 
ACCTACAGCC GTCAAGATGG AGTGGCCAAC ATCCCGATCA AACGTCAGAT CATAGAGGAT 
GGACGGGCAC AAGTCACATA CAGCACAGCA GACCTCACTG CTAAAGACAA GAAGGACTAT 
ATTTCGGTAG ATGGAGACTT GAGCTATGGA GCAGGTGAAA CAGAGAAGAT TGTCCCAGTG 
AAACTTCTGG AACTTGGAGA GCAAGATGGC CTGCTGGAGG ACAAGCAGGT CAAACAGTTT 
GTCATGGATT TGACCAACCC ACGACAAAGT GCTAAACTAG GCCGTTACCC ACGCACTGTT 
ATCACCATCG CTGACAAACC AGAATCCAGT GTTATGGCGT TTAAGAAGAG TACTCAAACT 
TTCAGTACCA CCGACTCGCT CTACACCATC CCGGTGGAAC GCACTGGAAA CCGGGAGACT 
CCGGCCACTG TGCACTGGCG CACCAACAAG GCCTCGCGCT TCGACATCTC CAGCCCTCTC 
AAATTTGCAC CCGGTGAAGC TGAAAAAAAT ATCCTCATCA ATCCTCGTAC ACATCCATCT 
CCCATCATAC CTGAGAAATT TAACCTGGAA CTATTAGACC CAAGCAACAA TGCAATTATC 
GGAAAGAGGA AGACCACGGA GGTGATCGTC ACCGACGGTC GAGGGGATGG TAAAAATCAG 
GATTTCCAGA AGATGGAATA TGTTACTCAA ACAGCCACAT CCCCCGGAGG ACGCCTCTAT 
TCTCCAGCCA ACATTAAAGC CGTTGCTACC GGCCCTAAAA ACATCCGCCT GAACTGGAAA 
CCCAGCCAAA ATGCCAATGG TTACAAGGTG AAGTACTGGA TTTATGGAGA CCCCGAAGAT 
AAAGCTAAAT TTATGGATGT GAAGACGCCT CAAGCCGAGC TGACAAATCT CGATCCATAC 
TGTGATTACG AGATGCGTGT TTGTGGCTAC AATGCACTTG GAGATGGCAA TTACAGTGAT 
ATCATCCAAT GCCAAACCCT GGAGGATATA CCTGGTGAAC CTGGTCGTCT GGCTTTCAAC 
GTCATCAGCC CTACTGTTAC TCAGGTTAGT TGGGCGGAGC CAGCAGAAGC CAATGGTATC 
ATCACTGGCT ATGAGGTCAT CTACATGCCC ATTAACGAGG ACAAGAAGCC TACAGGACCT 
CCTAAGAAGG TGATGATTGA CAACCCTAAA AAGCGCATGT TGCTCATTGA GAACCTGCAA 
CCTTCTCAGA CCTACTGTTA TAAAGTGCGT GCCAGCAATA AGGTGGGCTG GGGCCCATAC 
AGAGAAGCCA CAATCAACCT GGCATCACAG CCTACCAGAC CCATGTCAAT TCCCATCATT 
CCCGACATTC CCATCGTGGA TGCAGAAGCT GGCGAAGAGT ACGACAGCTA TCTGATGTAC 
AGTAATGAAG TCCTGCGATC CCCAACGAGC AGCCTGAGGC CTAGCGTCAC AGATCTGTCT 
GATGATCAGT TCGTGAACGG AAAGTGGGAA CAGAATTTCT TGTTTCCTGG AGGAGGAGGT 
GGTTCCCTCT CCCGCAACAT CAGCTCTTCC TCAACCACAT ACAGCCATTC CACACCGCGT 
ACCAATGTGA CCAACAACAG CTCCACCACA ACGTACATCT CTGGCAAAGG AGGCTCAACT 
ACACACAGAT ATGATGTAAG AGACGGTGGA CTTCTCAAAG AAGACGTGAT CCTGAGAAAA 
CGCTCTGAAA ACAGGCATTA CACAGAAGGT GATGGTGTGA GGGACTCAAT CGTAATGGGA 
AACCTGTCTG GTGGACTTTT GGACAATTTA GGGGTCTCTA GCTTCAGCCA GACTACATCT 
ACCAGCTACA GCTTGAGCTC CAGAGCTCGT ACTCAGTCTG AAGATATCAA CGATGCTCTG 
CTGAACCTGG ATAGTGTTCT TCAGGAATCG CGCTTCACCC CGGGTGTTCC CGCGACTCCC 
AGCAGACTGG TGTTTTCAGC TCTGGGCCAC ACGGCTCTCA AAGTGAGCTG GCAGGAGCCA 
CACTGCGAGT CTGATGTACT GGGATACTGC GTCCTTTACC AGCTCATCAA TGGAGGTGAC 
GTAAAACGTA TCGAGGTGAA CAATCCTGCT CAGAACTCAG TGCTGGTTCA GGATCTCCTG 
CCTAACCAGT CCTACATCTT CAAAGTCAAA GCACAGAGTC GAGAGGGCTG GGGTCCTGAG 
AGAGAGGGCG TCATTACCAT TGAGTCCAAC GTCGACCCCA AGAGTCCACT GAGCCCAGTG 
CCCGGGTCTC CGTTCACTCT CAGCACTCCC AGCGCTCCAG GACCTCTGAT CTTCACAGCG 
TTACGTCCAG ATACACTGCA GCTGAGCTGG GAGAAACCCC GAAAACCCAA CGGAGACATC 
CTGGGATACG TGGTGACCTG CGAACAGCTG CATGGTGGAG GAGATCTTCG CTCCTTCCAG 
GTGAATGGCA ATGCAGCCAC TTCATTGACT GTCTCTGACC TGAAAGAAAA CATGCCTTAC 
AAATTCAAAG TTCAAGCTCA GACCACGCAG GGCTTTGGCC CAGAGCGAGA GGGAATCATC 
ACCATCGAGT CCCAGGATGG AGGCTCCATG TCCCAATACA GCAGTCAGTC GGTCACGAAG 
AGAGAGGTGT TCAATCTGCC CACAGAAATC ACACACACTT CACACACCAT GTTCTCAGAG 
CCCTTTGTTT CTTCAGACGG GATGATGATG ATGTCGAGCA GGCAGATCAC AGAGTCGGGT 
GGCTCCATGA CACGGCAGGT GGAGATGGTG CAGAGAGGAG TGATGTCCAG CAGCACCGTC 
ACCAAGAGGC TGGAGAGACA GTACTACGAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166847.1
CDS	483..6278
Translation	MGGWAIRLAVGIFLLLLAATDSRADEVNHCTAARTKTCSECIQVGAGCAYCPDEVFEHERCDLKENLEAQKCSKIVNFESSRQIQENVKINEQLKQSQVSPQLMSMTLLPGEEKEVEMEVFEPTRGPLDLYILMDFSNSMSDDLDNLKRMGEKLARLVGDLSDDYTIGFGKFVDKVTEPQTDMRPAKLAEPWSNSDPPFSFRNVIKLTSNITSFRQKLQKERISGNLDAPEGGFDAILQTAVCQDQIGWRKDSTHLLVFSTESAFHYEADGANVLAGILDRNDEQCHLNVDGNYTHDVRQDYPSIPTLVRLLVKHNIIPIFAVTNHSYSYYEKLHEYFPIAELGQLQEDSSNILSILEKAFENIRSKISIRAEDRPKAVETKIFSQSGTFSEYGNFKITPGQTGKFKVRMKALNQVGEEPVCKVNQADRAGTLRVKPTTFSSAFKINAEVLCPTCDCEKTPVKNAVRCTGHGDLVCGKCQCYAGWLGPLCNCSAGASTDSVMCIERGKTEPCSGRGDCMCGTCVCYNPNQFEGPYCQFDKSQCQRFGGFLCNERGSCSMGRCVCSPGWSGEACECPTSNDSCRDSKGGICNDRGVCKCGRCECEHSGLPLSPTCEANFQQQVGMCEAKRSCVQCQAWSTGERKGRKCDECPFKIVRVDELEEDKNVIETCSFRDEDDDCTYHYNVNYPSNITDKEHRVLVKKKKDCPPAGFLWLIPLIMFLMLLLGLLLLCCWKYCACCQSCLALLPCCARGRMVGFKENQYLLRQSFLQNDYLDTPMVRSGPLKSTDVVRWKVADNVHRGTNHPQNQIRPNPKETIQYPVSLRLNRQFSEVLSNPDARDTDMLRREVEDNLNDVFLQIPGAQPVQKTLFRTQRNSGKRQNNTIVDTVLSAPRSSYPDIVKLTDKQVQSGNFRDLKVIPGYYTVASDKEAAGAVEFPENVESVDVRVPLFIKDEDDEKKELLVQAVDVPVGIAKIGKDHVNITVLKEQASSVFTFLEPSYTYSRQDGVANIPIKRQIIEDGRAQVTYSTADLTAKDKKDYISVDGDLSYGAGETEKIVPVKLLELGEQDGLLEDKQVKQFVMDLTNPRQSAKLGRYPRTVITIADKPESSVMAFKKSTQTFSTTDSLYTIPVERTGNRETPATVHWRTNKASRFDISSPLKFAPGEAEKNILINPRTHPSPIIPEKFNLELLDPSNNAIIGKRKTTEVIVTDGRGDGKNQDFQKMEYVTQTATSPGGRLYSPANIKAVATGPKNIRLNWKPSQNANGYKVKYWIYGDPEDKAKFMDVKTPQAELTNLDPYCDYEMRVCGYNALGDGNYSDIIQCQTLEDIPGEPGRLAFNVISPTVTQVSWAEPAEANGIITGYEVIYMPINEDKKPTGPPKKVMIDNPKKRMLLIENLQPSQTYCYKVRASNKVGWGPYREATINLASQPTRPMSIPIIPDIPIVDAEAGEEYDSYLMYSNEVLRSPTSSLRPSVTDLSDDQFVNGKWEQNFLFPGGGGGSLSRNISSSSTTYSHSTPRTNVTNNSSTTTYISGKGGSTTHRYDVRDGGLLKEDVILRKRSENRHYTEGDGVRDSIVMGNLSGGLLDNLGVSSFSQTTSTSYSLSSRARTQSEDINDALLNLDSVLQESRFTPGVPATPSRLVFSALGHTALKVSWQEPHCESDVLGYCVLYQLINGGDVKRIEVNNPAQNSVLVQDLLPNQSYIFKVKAQSREGWGPEREGVITIESNVDPKSPLSPVPGSPFTLSTPSAPGPLIFTALRPDTLQLSWEKPRKPNGDILGYVVTCEQLHGGGDLRSFQVNGNAATSLTVSDLKENMPYKFKVQAQTTQGFGPEREGIITIESQDGGSMSQYSSQSVTKREVFNLPTEITHTSHTMFSEPFVSSDGMMMMSSRQITESGGSMTRQVEMVQRGVMSSSTVTKRLERQYYEA

Target ORF information:

RefSeq Version	XM_005166790.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio integrin, beta 4 (itgb4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166790.3

ORF Insert Sequence:

ATGGGAGGAT GGGCGATACG TCTGGCTGTG GGGATCTTCC TTCTCTTGCT TGCTGCGACA 
GACAGTCGTG CTGATGAAGT GAATCACTGC ACTGCTGCCA GGACCAAAAC CTGCTCAGAA 
TGTATTCAGG TTGGCGCAGG ATGTGCGTAC TGCCCTGATG AGGTCTTCGA GCATGAGCGT 
TGTGACCTGA AAGAAAACCT GGAGGCCCAA AAATGTAGCA AAATAGTAAA TTTTGAGAGC 
AGCAGGCAAA TCCAAGAGAA TGTGAAAATC AACGAGCAAC TGAAGCAGTC TCAGGTGTCC 
CCTCAGCTCA TGAGCATGAC GCTTCTGCCC GGGGAGGAGA AAGAGGTGGA GATGGAGGTG 
TTTGAACCCA CCCGTGGCCC ACTGGATCTC TACATCCTCA TGGACTTCTC CAACTCCATG 
TCTGACGATC TGGACAACCT TAAGAGGATG GGAGAAAAGC TGGCACGGCT TGTTGGCGAT 
CTCTCTGACG ATTACACCAT CGGATTTGGC AAGTTTGTGG ACAAAGTCAC CGAGCCTCAG 
ACAGACATGA GGCCAGCCAA ATTAGCTGAG CCGTGGTCCA ATAGCGACCC TCCATTTTCC 
TTCCGTAACG TCATCAAGCT CACCAGCAAC ATCACCTCCT TCAGACAGAA GCTACAGAAA 
GAGCGTATAT CTGGCAACCT TGATGCTCCA GAAGGTGGTT TTGATGCCAT CTTACAGACT 
GCTGTCTGCC AGGACCAAAT TGGCTGGAGG AAAGACAGCA CACACTTGCT GGTCTTCTCC 
ACTGAATCAG CTTTCCATTA TGAGGCAGAT GGTGCCAACG TGCTCGCTGG CATCTTGGAC 
CGCAATGATG AGCAATGCCA TCTGAATGTA GATGGAAACT ACACGCATGA TGTTCGTCAA 
GATTACCCAT CTATACCGAC TCTAGTGCGA CTTTTGGTGA AACATAACAT CATCCCAATT 
TTCGCTGTTA CTAATCACTC ATACTCATAC TATGAGAAAC TTCATGAATA CTTCCCCATT 
GCTGAGCTGG GACAGTTACA GGAGGATTCA TCTAATATCC TGAGCATTTT AGAAAAAGCT 
TTTGAGAATA TTCGTTCCAA GATCAGTATT CGTGCAGAGG ACAGACCAAA GGCAGTGGAA 
ACCAAGATTT TCTCTCAGTC TGGCACCTTT TCTGAATATG GCAATTTCAA AATCACACCA 
GGACAAACTG GTAAATTTAA AGTGCGTATG AAGGCCCTTA ATCAGGTAGG AGAAGAGCCC 
GTGTGTAAGG TCAACCAGGC TGACCGCGCA GGAACCTTGA GAGTCAAGCC CACCACCTTC 
AGCTCTGCCT TTAAAATCAA TGCAGAGGTG CTCTGTCCAA CCTGTGACTG TGAAAAGACC 
CCGGTTAAAA ATGCAGTGCG CTGTACTGGC CATGGAGATC TGGTCTGTGG GAAATGCCAG 
TGCTATGCAG GATGGCTCGG CCCTTTGTGT AACTGTTCAG CGGGCGCCTC CACAGACTCT 
GTCATGTGCA TAGAACGAGG AAAGACAGAG CCGTGCTCAG GCAGAGGAGA CTGCATGTGT 
GGGACCTGTG TGTGTTATAA CCCCAACCAG TTTGAAGGGC CCTACTGCCA GTTTGACAAA 
TCTCAGTGCC AGAGATTTGG TGGATTTCTC TGTAATGAGC GTGGAAGCTG CAGCATGGGC 
AGATGTGTTT GTTCTCCGGG ATGGAGTGGA GAGGCCTGTG AATGTCCCAC CAGCAATGAC 
TCATGCCGGG ATAGCAAAGG GGGCATCTGT AATGACCGTG GTGTGTGTAA ATGTGGCCGC 
TGTGAGTGTG AACACTCAGG ACTTCCACTG AGTCCTACCT GCGAAGCCAA CTTTCAGCAA 
CAAGTGGGTA TGTGTGAAGC CAAGAGGAGC TGCGTCCAGT GCCAGGCCTG GAGCACCGGT 
GAAAGGAAAG GACGGAAATG CGATGAGTGT CCCTTCAAAA TTGTCAGGGT GGATGAGCTT 
GAAGAAGATA AAAATGTAAT CGAGACATGC AGTTTCCGTG ATGAAGATGA TGACTGCACA 
TATCACTATA ATGTGAATTA TCCATCGAAC ATCACAGACA AAGAACACCG CGTCCTAGTG 
AAGAAAAAGA AAGATTGTCC ACCTGCAGGT TTCCTGTGGC TCATTCCTCT CATTATGTTT 
CTGATGCTGT TACTTGGTTT GCTTTTGCTC TGTTGCTGGA AATACTGCGC CTGCTGCCAG 
TCCTGTCTTG CTCTTCTCCC GTGCTGTGCA AGAGGTCGTA TGGTGGGCTT TAAAGAAAAC 
CAGTACCTGC TACGTCAGTC TTTTCTGCAA AATGATTATT TGGACACCCC AATGGTGCGC 
AGCGGCCCGC TCAAGAGCAC AGACGTTGTG CGCTGGAAAG TGGCAGACAA TGTTCATCGA 
GGAACGAATC ACCCCCAGAA CCAAATCAGA CCCAACCCCA AAGAGACCAT CCAGTATCCT 
GTTTCTCTTC GTCTGAACCG CCAGTTTTCA GAAGTTCTTT CTAACCCTGA CGCTCGGGAC 
ACAGACATGC TCAGACGAGA AGTAGAGGAC AACCTCAATG ACGTGTTCCT GCAGATCCCG 
GGCGCACAAC CAGTGCAGAA GACTCTGTTC AGGACACAAA GAAATTCTGG AAAGAGACAG 
AACAACACAA TTGTGGACAC AGTTTTATCT GCCCCTCGCT CCAGTTACCC AGATATTGTA 
AAACTGACAG ATAAACAGGT TCAGTCTGGA AACTTCCGGG ACCTGAAGGT GATTCCAGGC 
TACTACACTG TGGCTTCTGA TAAAGAAGCA GCAGGCGCAG TGGAGTTTCC GGAGAACGTG 
GAATCAGTGG ATGTTCGGGT TCCTCTCTTC ATTAAAGATG AGGACGATGA AAAGAAGGAG 
CTGCTTGTGC AAGCTGTGGA TGTTCCAGTC GGAATCGCAA AGATTGGAAA AGATCATGTC 
AACATTACAG TGCTTAAGGA ACAGGCTTCG AGTGTCTTTA CCTTCCTGGA GCCGTCATAT 
ACCTACAGCC GTCAAGATGG AGTGGCCAAC ATCCCGATCA AACGTCAGAT CATAGAGGAT 
GGACGGGCAC AAGTCACATA CAGCACAGCA GACCTCACTG CTAAAGACAA GAAGGACTAT 
ATTTCGGTAG ATGGAGACTT GAGCTATGGA GCAGGTGAAA CAGAGAAGAT TGTCCCAGTG 
AAACTTCTGG AACTTGGAGA GCAAGATGGC CTGCTGGAGG ACAAGCAGGT CAAACAGTTT 
GTCATGGATT TGACCAACCC ACGACAAAGT GCTAAACTAG GCCGTTACCC ACGCACTGTT 
ATCACCATCG CTGACAAACC AGAATCCAGT GTTATGGCGT TTAAGAAGAG TACTCAAACT 
TTCAGTACCA CCGACTCGCT CTACACCATC CCGGTGGAAC GCACTGGAAA CCGGGAGACT 
CCGGCCACTG TGCACTGGCG CACCAACAAG GCCTCGCGCT TCGACATCTC CAGCCCTCTC 
AAATTTGCAC CCGGTGAAGC TGAAAAAAAT ATCCTCATCA ATCCTCGTAC ACATCCATCT 
CCCATCATAC CTGAGAAATT TAACCTGGAA CTATTAGACC CAAGCAACAA TGCAATTATC 
GGAAAGAGGA AGACCACGGA GGTGATCGTC ACCGACGGTC GAGGGGATGG TAAAAATCAG 
GATTTCCAGA AGATGGAATA TGTTACTCAA ACAGCCACAT CCCCCGGAGG ACGCCTCTAT 
TCTCCAGCCA ACATTAAAGC CGTTGCTACC GGCCCTAAAA ACATCCGCCT GAACTGGAAA 
CCCAGCCAAA ATGCCAATGG TTACAAGGTG AAGTACTGGA TTTATGGAGA CCCCGAAGAT 
AAAGCTAAAT TTATGGATGT GAAGACGCCT CAAGCCGAGC TGACAAATCT CGATCCATAC 
TGTGATTACG AGATGCGTGT TTGTGGCTAC AATGCACTTG GAGATGGCAA TTACAGTGAT 
ATCATCCAAT GCCAAACCCT GGAGGATATA CCTGGTGAAC CTGGTCGTCT GGCTTTCAAC 
GTCATCAGCC CTACTGTTAC TCAGGTTAGT TGGGCGGAGC CAGCAGAAGC CAATGGTATC 
ATCACTGGCT ATGAGGTCAT CTACATGCCC ATTAACGAGG ACAAGAAGCC TACAGGACCT 
CCTAAGAAGG TGATGATTGA CAACCCTAAA AAGCGCATGT TGCTCATTGA GAACCTGCAA 
CCTTCTCAGA CCTACTGTTA TAAAGTGCGT GCCAGCAATA AGGTGGGCTG GGGCCCATAC 
AGAGAAGCCA CAATCAACCT GGCATCACAG CCTACCAGAC CCATGTCAAT TCCCATCATT 
CCCGACATTC CCATCGTGGA TGCAGAAGCT GGCGAAGAGT ACGACAGCTA TCTGATGTAC 
AGTAATGAAG TCCTGCGATC CCCAACGAGC AGCCTGAGGC CTAGCGTCAC AGATCTGTCT 
GATGATCAGT TCGTGAACGG AAAGTGGGAA CAGAATTTCT TGTTTCCTGG AGGAGGAGGT 
GGTTCCCTCT CCCGCAACAT CAGCTCTTCC TCAACCACAT ACAGCCATTC CACACCGCGT 
ACCAATGTGA CCAACAACAG CTCCACCACA ACGTACATCT CTGGCAAAGG AGGCTCAACT 
ACACACAGAT ATGATGTAAG AGACGGTGGA CTTCTCAAAG AAGACGTGAT CCTGAGAAAA 
CGCTCTGAAA ACAGGCATTA CACAGAAGGT GATGGTGTGA GGGACTCAAT CGTAATGGGA 
AACCTGTCTG GTGGACTTTT GGACAATTTA GGGGTCTCTA GCTTCAGCCA GACTACATCT 
ACCAGCTACA GCTTGAGCTC CAGAGCTCGT ACTCAGTCTG AAGATATCAA CGATGCTCTG 
CTGAACCTGG ATAGTGTTCT TCAGGAATCG CGCTTCACCC CGGGTGTTCC CGCGACTCCC 
AGCAGACTGG TGTTTTCAGC TCTGGGCCAC ACGGCTCTCA AAGTGAGCTG GCAGGAGCCA 
CACTGCGAGT CTGATGTACT GGGATACTGC GTCCTTTACC AGCTCATCAA TGGAGGTGAC 
GTAAAACGTA TCGAGGTGAA CAATCCTGCT CAGAACTCAG TGCTGGTTCA GGATCTCCTG 
CCTAACCAGT CCTACATCTT CAAAGTCAAA GCACAGAGTC GAGAGGGCTG GGGTCCTGAG 
AGAGAGGGCG TCATTACCAT TGAGTCCAAC GTCGACCCCA AGAGTCCACT GAGCCCAGTG 
CCCGGGTCTC CGTTCACTCT CAGCACTCCC AGCGCTCCAG GACCTCTGAT CTTCACAGCG 
TTACGTCCAG ATACACTGCA GCTGAGCTGG GAGAAACCCC GAAAACCCAA CGGAGACATC 
CTGGGATACG TGGTGACCTG CGAACAGCTG CATGGTGGAG GAGATCTTCG CTCCTTCCAG 
GTGAATGGCA ATGCAGCCAC TTCATTGACT GTCTCTGACC TGAAAGAAAA CATGCCTTAC 
AAATTCAAAG TTCAAGCTCA GACCACGCAG GGCTTTGGCC CAGAGCGAGA GGGAATCATC 
ACCATCGAGT CCCAGGATGG AGGCTCCATG TCCCAATACA GCAGTCAGTC GGTCACGAAG 
AGAGAGGTGT TCAATCTGCC CACAGAAATC ACACACACTT CACACACCAT GTTCTCAGAG 
CCCTTTGTTT CTTCAGACGG GATGATGATG ATGTCGAGCA GGCAGATCAC AGAGTCGGGT 
GGCTCCATGA CACGGCAGGT GGAGATGGTG CAGAGAGGAG TGATGTCCAG CAGCACCGTC 
ACCAAGAGGC TGGAGAGACA GTACTACGAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itgb4 ( XM_005166791.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166848.1 Danio rerio integrin, beta 4 (itgb4), transcript variant X2, mRNA.

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CloneID	ODa13938
Clone ID Related Accession (Same CDS sequence)	XM_005166790.4 , XM_005166791.3 , XM_005166790.3 , XM_005166791.4
Accession Version	XM_005166791.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	integrin beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166791.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGGAT GGGCGATACG TCTGGCTGTG GGGATCTTCC TTCTCTTGCT TGCTGCGACA 
GACAGTCGTG CTGATGAAGT GAATCACTGC ACTGCTGCCA GGACCAAAAC CTGCTCAGAA 
TGTATTCAGG TTGGCGCAGG ATGTGCGTAC TGCCCTGATG AGGTCTTCGA GCATGAGCGT 
TGTGACCTGA AAGAAAACCT GGAGGCCCAA AAATGTAGCA AAATAGTAAA TTTTGAGAGC 
AGCAGGCAAA TCCAAGAGAA TGTGAAAATC AACGAGCAAC TGAAGCAGTC TCAGGTGTCC 
CCTCAGCTCA TGAGCATGAC GCTTCTGCCC GGGGAGGAGA AAGAGGTGGA GATGGAGGTG 
TTTGAACCCA CCCGTGGCCC ACTGGATCTC TACATCCTCA TGGACTTCTC CAACTCCATG 
TCTGACGATC TGGACAACCT TAAGAGGATG GGAGAAAAGC TGGCACGGCT TGTTGGCGAT 
CTCTCTGACG ATTACACCAT CGGATTTGGC AAGTTTGTGG ACAAAGTCAC CGAGCCTCAG 
ACAGACATGA GGCCAGCCAA ATTAGCTGAG CCGTGGTCCA ATAGCGACCC TCCATTTTCC 
TTCCGTAACG TCATCAAGCT CACCAGCAAC ATCACCTCCT TCAGACAGAA GCTACAGAAA 
GAGCGTATAT CTGGCAACCT TGATGCTCCA GAAGGTGGTT TTGATGCCAT CTTACAGACT 
GCTGTCTGCC AGGACCAAAT TGGCTGGAGG AAAGACAGCA CACACTTGCT GGTCTTCTCC 
ACTGAATCAG CTTTCCATTA TGAGGCAGAT GGTGCCAACG TGCTCGCTGG CATCTTGGAC 
CGCAATGATG AGCAATGCCA TCTGAATGTA GATGGAAACT ACACGCATGA TGTTCGTCAA 
GATTACCCAT CTATACCGAC TCTAGTGCGA CTTTTGGTGA AACATAACAT CATCCCAATT 
TTCGCTGTTA CTAATCACTC ATACTCATAC TATGAGAAAC TTCATGAATA CTTCCCCATT 
GCTGAGCTGG GACAGTTACA GGAGGATTCA TCTAATATCC TGAGCATTTT AGAAAAAGCT 
TTTGAGAATA TTCGTTCCAA GATCAGTATT CGTGCAGAGG ACAGACCAAA GGCAGTGGAA 
ACCAAGATTT TCTCTCAGTC TGGCACCTTT TCTGAATATG GCAATTTCAA AATCACACCA 
GGACAAACTG GTAAATTTAA AGTGCGTATG AAGGCCCTTA ATCAGGTAGG AGAAGAGCCC 
GTGTGTAAGG TCAACCAGGC TGACCGCGCA GGAACCTTGA GAGTCAAGCC CACCACCTTC 
AGCTCTGCCT TTAAAATCAA TGCAGAGGTG CTCTGTCCAA CCTGTGACTG TGAAAAGACC 
CCGGTTAAAA ATGCAGTGCG CTGTACTGGC CATGGAGATC TGGTCTGTGG GAAATGCCAG 
TGCTATGCAG GATGGCTCGG CCCTTTGTGT AACTGTTCAG CGGGCGCCTC CACAGACTCT 
GTCATGTGCA TAGAACGAGG AAAGACAGAG CCGTGCTCAG GCAGAGGAGA CTGCATGTGT 
GGGACCTGTG TGTGTTATAA CCCCAACCAG TTTGAAGGGC CCTACTGCCA GTTTGACAAA 
TCTCAGTGCC AGAGATTTGG TGGATTTCTC TGTAATGAGC GTGGAAGCTG CAGCATGGGC 
AGATGTGTTT GTTCTCCGGG ATGGAGTGGA GAGGCCTGTG AATGTCCCAC CAGCAATGAC 
TCATGCCGGG ATAGCAAAGG GGGCATCTGT AATGACCGTG GTGTGTGTAA ATGTGGCCGC 
TGTGAGTGTG AACACTCAGG ACTTCCACTG AGTCCTACCT GCGAAGCCAA CTTTCAGCAA 
CAAGTGGGTA TGTGTGAAGC CAAGAGGAGC TGCGTCCAGT GCCAGGCCTG GAGCACCGGT 
GAAAGGAAAG GACGGAAATG CGATGAGTGT CCCTTCAAAA TTGTCAGGGT GGATGAGCTT 
GAAGAAGATA AAAATGTAAT CGAGACATGC AGTTTCCGTG ATGAAGATGA TGACTGCACA 
TATCACTATA ATGTGAATTA TCCATCGAAC ATCACAGACA AAGAACACCG CGTCCTAGTG 
AAGAAAAAGA AAGATTGTCC ACCTGCAGGT TTCCTGTGGC TCATTCCTCT CATTATGTTT 
CTGATGCTGT TACTTGGTTT GCTTTTGCTC TGTTGCTGGA AATACTGCGC CTGCTGCCAG 
TCCTGTCTTG CTCTTCTCCC GTGCTGTGCA AGAGGTCGTA TGGTGGGCTT TAAAGAAAAC 
CAGTACCTGC TACGTCAGTC TTTTCTGCAA AATGATTATT TGGACACCCC AATGGTGCGC 
AGCGGCCCGC TCAAGAGCAC AGACGTTGTG CGCTGGAAAG TGGCAGACAA TGTTCATCGA 
GGAACGAATC ACCCCCAGAA CCAAATCAGA CCCAACCCCA AAGAGACCAT CCAGTATCCT 
GTTTCTCTTC GTCTGAACCG CCAGTTTTCA GAAGTTCTTT CTAACCCTGA CGCTCGGGAC 
ACAGACATGC TCAGACGAGA AGTAGAGGAC AACCTCAATG ACGTGTTCCT GCAGATCCCG 
GGCGCACAAC CAGTGCAGAA GACTCTGTTC AGGACACAAA GAAATTCTGG AAAGAGACAG 
AACAACACAA TTGTGGACAC AGTTTTATCT GCCCCTCGCT CCAGTTACCC AGATATTGTA 
AAACTGACAG ATAAACAGGT TCAGTCTGGA AACTTCCGGG ACCTGAAGGT GATTCCAGGC 
TACTACACTG TGGCTTCTGA TAAAGAAGCA GCAGGCGCAG TGGAGTTTCC GGAGAACGTG 
GAATCAGTGG ATGTTCGGGT TCCTCTCTTC ATTAAAGATG AGGACGATGA AAAGAAGGAG 
CTGCTTGTGC AAGCTGTGGA TGTTCCAGTC GGAATCGCAA AGATTGGAAA AGATCATGTC 
AACATTACAG TGCTTAAGGA ACAGGCTTCG AGTGTCTTTA CCTTCCTGGA GCCGTCATAT 
ACCTACAGCC GTCAAGATGG AGTGGCCAAC ATCCCGATCA AACGTCAGAT CATAGAGGAT 
GGACGGGCAC AAGTCACATA CAGCACAGCA GACCTCACTG CTAAAGACAA GAAGGACTAT 
ATTTCGGTAG ATGGAGACTT GAGCTATGGA GCAGGTGAAA CAGAGAAGAT TGTCCCAGTG 
AAACTTCTGG AACTTGGAGA GCAAGATGGC CTGCTGGAGG ACAAGCAGGT CAAACAGTTT 
GTCATGGATT TGACCAACCC ACGACAAAGT GCTAAACTAG GCCGTTACCC ACGCACTGTT 
ATCACCATCG CTGACAAACC AGAATCCAGT GTTATGGCGT TTAAGAAGAG TACTCAAACT 
TTCAGTACCA CCGACTCGCT CTACACCATC CCGGTGGAAC GCACTGGAAA CCGGGAGACT 
CCGGCCACTG TGCACTGGCG CACCAACAAG GCCTCGCGCT TCGACATCTC CAGCCCTCTC 
AAATTTGCAC CCGGTGAAGC TGAAAAAAAT ATCCTCATCA ATCCTCGTAC ACATCCATCT 
CCCATCATAC CTGAGAAATT TAACCTGGAA CTATTAGACC CAAGCAACAA TGCAATTATC 
GGAAAGAGGA AGACCACGGA GGTGATCGTC ACCGACGGTC GAGGGGATGG TAAAAATCAG 
GATTTCCAGA AGATGGAATA TGTTACTCAA ACAGCCACAT CCCCCGGAGG ACGCCTCTAT 
TCTCCAGCCA ACATTAAAGC CGTTGCTACC GGCCCTAAAA ACATCCGCCT GAACTGGAAA 
CCCAGCCAAA ATGCCAATGG TTACAAGGTG AAGTACTGGA TTTATGGAGA CCCCGAAGAT 
AAAGCTAAAT TTATGGATGT GAAGACGCCT CAAGCCGAGC TGACAAATCT CGATCCATAC 
TGTGATTACG AGATGCGTGT TTGTGGCTAC AATGCACTTG GAGATGGCAA TTACAGTGAT 
ATCATCCAAT GCCAAACCCT GGAGGATATA CCTGGTGAAC CTGGTCGTCT GGCTTTCAAC 
GTCATCAGCC CTACTGTTAC TCAGGTTAGT TGGGCGGAGC CAGCAGAAGC CAATGGTATC 
ATCACTGGCT ATGAGGTCAT CTACATGCCC ATTAACGAGG ACAAGAAGCC TACAGGACCT 
CCTAAGAAGG TGATGATTGA CAACCCTAAA AAGCGCATGT TGCTCATTGA GAACCTGCAA 
CCTTCTCAGA CCTACTGTTA TAAAGTGCGT GCCAGCAATA AGGTGGGCTG GGGCCCATAC 
AGAGAAGCCA CAATCAACCT GGCATCACAG CCTACCAGAC CCATGTCAAT TCCCATCATT 
CCCGACATTC CCATCGTGGA TGCAGAAGCT GGCGAAGAGT ACGACAGCTA TCTGATGTAC 
AGTAATGAAG TCCTGCGATC CCCAACGAGC AGCCTGAGGC CTAGCGTCAC AGATCTGTCT 
GATGATCAGT TCGTGAACGG AAAGTGGGAA CAGAATTTCT TGTTTCCTGG AGGAGGAGGT 
GGTTCCCTCT CCCGCAACAT CAGCTCTTCC TCAACCACAT ACAGCCATTC CACACCGCGT 
ACCAATGTGA CCAACAACAG CTCCACCACA ACGTACATCT CTGGCAAAGG AGGCTCAACT 
ACACACAGAT ATGATGTAAG AGACGGTGGA CTTCTCAAAG AAGACGTGAT CCTGAGAAAA 
CGCTCTGAAA ACAGGCATTA CACAGAAGGT GATGGTGTGA GGGACTCAAT CGTAATGGGA 
AACCTGTCTG GTGGACTTTT GGACAATTTA GGGGTCTCTA GCTTCAGCCA GACTACATCT 
ACCAGCTACA GCTTGAGCTC CAGAGCTCGT ACTCAGTCTG AAGATATCAA CGATGCTCTG 
CTGAACCTGG ATAGTGTTCT TCAGGAATCG CGCTTCACCC CGGGTGTTCC CGCGACTCCC 
AGCAGACTGG TGTTTTCAGC TCTGGGCCAC ACGGCTCTCA AAGTGAGCTG GCAGGAGCCA 
CACTGCGAGT CTGATGTACT GGGATACTGC GTCCTTTACC AGCTCATCAA TGGAGGTGAC 
GTAAAACGTA TCGAGGTGAA CAATCCTGCT CAGAACTCAG TGCTGGTTCA GGATCTCCTG 
CCTAACCAGT CCTACATCTT CAAAGTCAAA GCACAGAGTC GAGAGGGCTG GGGTCCTGAG 
AGAGAGGGCG TCATTACCAT TGAGTCCAAC GTCGACCCCA AGAGTCCACT GAGCCCAGTG 
CCCGGGTCTC CGTTCACTCT CAGCACTCCC AGCGCTCCAG GACCTCTGAT CTTCACAGCG 
TTACGTCCAG ATACACTGCA GCTGAGCTGG GAGAAACCCC GAAAACCCAA CGGAGACATC 
CTGGGATACG TGGTGACCTG CGAACAGCTG CATGGTGGAG GAGATCTTCG CTCCTTCCAG 
GTGAATGGCA ATGCAGCCAC TTCATTGACT GTCTCTGACC TGAAAGAAAA CATGCCTTAC 
AAATTCAAAG TTCAAGCTCA GACCACGCAG GGCTTTGGCC CAGAGCGAGA GGGAATCATC 
ACCATCGAGT CCCAGGATGG AGGCTCCATG TCCCAATACA GCAGTCAGTC GGTCACGAAG 
AGAGAGGTGT TCAATCTGCC CACAGAAATC ACACACACTT CACACACCAT GTTCTCAGAG 
CCCTTTGTTT CTTCAGACGG GATGATGATG ATGTCGAGCA GGCAGATCAC AGAGTCGGGT 
GGCTCCATGA CACGGCAGGT GGAGATGGTG CAGAGAGGAG TGATGTCCAG CAGCACCGTC 
ACCAAGAGGC TGGAGAGACA GTACTACGAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166848.1
CDS	195..5990
Translation	MGGWAIRLAVGIFLLLLAATDSRADEVNHCTAARTKTCSECIQVGAGCAYCPDEVFEHERCDLKENLEAQKCSKIVNFESSRQIQENVKINEQLKQSQVSPQLMSMTLLPGEEKEVEMEVFEPTRGPLDLYILMDFSNSMSDDLDNLKRMGEKLARLVGDLSDDYTIGFGKFVDKVTEPQTDMRPAKLAEPWSNSDPPFSFRNVIKLTSNITSFRQKLQKERISGNLDAPEGGFDAILQTAVCQDQIGWRKDSTHLLVFSTESAFHYEADGANVLAGILDRNDEQCHLNVDGNYTHDVRQDYPSIPTLVRLLVKHNIIPIFAVTNHSYSYYEKLHEYFPIAELGQLQEDSSNILSILEKAFENIRSKISIRAEDRPKAVETKIFSQSGTFSEYGNFKITPGQTGKFKVRMKALNQVGEEPVCKVNQADRAGTLRVKPTTFSSAFKINAEVLCPTCDCEKTPVKNAVRCTGHGDLVCGKCQCYAGWLGPLCNCSAGASTDSVMCIERGKTEPCSGRGDCMCGTCVCYNPNQFEGPYCQFDKSQCQRFGGFLCNERGSCSMGRCVCSPGWSGEACECPTSNDSCRDSKGGICNDRGVCKCGRCECEHSGLPLSPTCEANFQQQVGMCEAKRSCVQCQAWSTGERKGRKCDECPFKIVRVDELEEDKNVIETCSFRDEDDDCTYHYNVNYPSNITDKEHRVLVKKKKDCPPAGFLWLIPLIMFLMLLLGLLLLCCWKYCACCQSCLALLPCCARGRMVGFKENQYLLRQSFLQNDYLDTPMVRSGPLKSTDVVRWKVADNVHRGTNHPQNQIRPNPKETIQYPVSLRLNRQFSEVLSNPDARDTDMLRREVEDNLNDVFLQIPGAQPVQKTLFRTQRNSGKRQNNTIVDTVLSAPRSSYPDIVKLTDKQVQSGNFRDLKVIPGYYTVASDKEAAGAVEFPENVESVDVRVPLFIKDEDDEKKELLVQAVDVPVGIAKIGKDHVNITVLKEQASSVFTFLEPSYTYSRQDGVANIPIKRQIIEDGRAQVTYSTADLTAKDKKDYISVDGDLSYGAGETEKIVPVKLLELGEQDGLLEDKQVKQFVMDLTNPRQSAKLGRYPRTVITIADKPESSVMAFKKSTQTFSTTDSLYTIPVERTGNRETPATVHWRTNKASRFDISSPLKFAPGEAEKNILINPRTHPSPIIPEKFNLELLDPSNNAIIGKRKTTEVIVTDGRGDGKNQDFQKMEYVTQTATSPGGRLYSPANIKAVATGPKNIRLNWKPSQNANGYKVKYWIYGDPEDKAKFMDVKTPQAELTNLDPYCDYEMRVCGYNALGDGNYSDIIQCQTLEDIPGEPGRLAFNVISPTVTQVSWAEPAEANGIITGYEVIYMPINEDKKPTGPPKKVMIDNPKKRMLLIENLQPSQTYCYKVRASNKVGWGPYREATINLASQPTRPMSIPIIPDIPIVDAEAGEEYDSYLMYSNEVLRSPTSSLRPSVTDLSDDQFVNGKWEQNFLFPGGGGGSLSRNISSSSTTYSHSTPRTNVTNNSSTTTYISGKGGSTTHRYDVRDGGLLKEDVILRKRSENRHYTEGDGVRDSIVMGNLSGGLLDNLGVSSFSQTTSTSYSLSSRARTQSEDINDALLNLDSVLQESRFTPGVPATPSRLVFSALGHTALKVSWQEPHCESDVLGYCVLYQLINGGDVKRIEVNNPAQNSVLVQDLLPNQSYIFKVKAQSREGWGPEREGVITIESNVDPKSPLSPVPGSPFTLSTPSAPGPLIFTALRPDTLQLSWEKPRKPNGDILGYVVTCEQLHGGGDLRSFQVNGNAATSLTVSDLKENMPYKFKVQAQTTQGFGPEREGIITIESQDGGSMSQYSSQSVTKREVFNLPTEITHTSHTMFSEPFVSSDGMMMMSSRQITESGGSMTRQVEMVQRGVMSSSTVTKRLERQYYEA

Target ORF information:

RefSeq Version	XM_005166791.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio integrin, beta 4 (itgb4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166791.4

ORF Insert Sequence:

ATGGGAGGAT GGGCGATACG TCTGGCTGTG GGGATCTTCC TTCTCTTGCT TGCTGCGACA 
GACAGTCGTG CTGATGAAGT GAATCACTGC ACTGCTGCCA GGACCAAAAC CTGCTCAGAA 
TGTATTCAGG TTGGCGCAGG ATGTGCGTAC TGCCCTGATG AGGTCTTCGA GCATGAGCGT 
TGTGACCTGA AAGAAAACCT GGAGGCCCAA AAATGTAGCA AAATAGTAAA TTTTGAGAGC 
AGCAGGCAAA TCCAAGAGAA TGTGAAAATC AACGAGCAAC TGAAGCAGTC TCAGGTGTCC 
CCTCAGCTCA TGAGCATGAC GCTTCTGCCC GGGGAGGAGA AAGAGGTGGA GATGGAGGTG 
TTTGAACCCA CCCGTGGCCC ACTGGATCTC TACATCCTCA TGGACTTCTC CAACTCCATG 
TCTGACGATC TGGACAACCT TAAGAGGATG GGAGAAAAGC TGGCACGGCT TGTTGGCGAT 
CTCTCTGACG ATTACACCAT CGGATTTGGC AAGTTTGTGG ACAAAGTCAC CGAGCCTCAG 
ACAGACATGA GGCCAGCCAA ATTAGCTGAG CCGTGGTCCA ATAGCGACCC TCCATTTTCC 
TTCCGTAACG TCATCAAGCT CACCAGCAAC ATCACCTCCT TCAGACAGAA GCTACAGAAA 
GAGCGTATAT CTGGCAACCT TGATGCTCCA GAAGGTGGTT TTGATGCCAT CTTACAGACT 
GCTGTCTGCC AGGACCAAAT TGGCTGGAGG AAAGACAGCA CACACTTGCT GGTCTTCTCC 
ACTGAATCAG CTTTCCATTA TGAGGCAGAT GGTGCCAACG TGCTCGCTGG CATCTTGGAC 
CGCAATGATG AGCAATGCCA TCTGAATGTA GATGGAAACT ACACGCATGA TGTTCGTCAA 
GATTACCCAT CTATACCGAC TCTAGTGCGA CTTTTGGTGA AACATAACAT CATCCCAATT 
TTCGCTGTTA CTAATCACTC ATACTCATAC TATGAGAAAC TTCATGAATA CTTCCCCATT 
GCTGAGCTGG GACAGTTACA GGAGGATTCA TCTAATATCC TGAGCATTTT AGAAAAAGCT 
TTTGAGAATA TTCGTTCCAA GATCAGTATT CGTGCAGAGG ACAGACCAAA GGCAGTGGAA 
ACCAAGATTT TCTCTCAGTC TGGCACCTTT TCTGAATATG GCAATTTCAA AATCACACCA 
GGACAAACTG GTAAATTTAA AGTGCGTATG AAGGCCCTTA ATCAGGTAGG AGAAGAGCCC 
GTGTGTAAGG TCAACCAGGC TGACCGCGCA GGAACCTTGA GAGTCAAGCC CACCACCTTC 
AGCTCTGCCT TTAAAATCAA TGCAGAGGTG CTCTGTCCAA CCTGTGACTG TGAAAAGACC 
CCGGTTAAAA ATGCAGTGCG CTGTACTGGC CATGGAGATC TGGTCTGTGG GAAATGCCAG 
TGCTATGCAG GATGGCTCGG CCCTTTGTGT AACTGTTCAG CGGGCGCCTC CACAGACTCT 
GTCATGTGCA TAGAACGAGG AAAGACAGAG CCGTGCTCAG GCAGAGGAGA CTGCATGTGT 
GGGACCTGTG TGTGTTATAA CCCCAACCAG TTTGAAGGGC CCTACTGCCA GTTTGACAAA 
TCTCAGTGCC AGAGATTTGG TGGATTTCTC TGTAATGAGC GTGGAAGCTG CAGCATGGGC 
AGATGTGTTT GTTCTCCGGG ATGGAGTGGA GAGGCCTGTG AATGTCCCAC CAGCAATGAC 
TCATGCCGGG ATAGCAAAGG GGGCATCTGT AATGACCGTG GTGTGTGTAA ATGTGGCCGC 
TGTGAGTGTG AACACTCAGG ACTTCCACTG AGTCCTACCT GCGAAGCCAA CTTTCAGCAA 
CAAGTGGGTA TGTGTGAAGC CAAGAGGAGC TGCGTCCAGT GCCAGGCCTG GAGCACCGGT 
GAAAGGAAAG GACGGAAATG CGATGAGTGT CCCTTCAAAA TTGTCAGGGT GGATGAGCTT 
GAAGAAGATA AAAATGTAAT CGAGACATGC AGTTTCCGTG ATGAAGATGA TGACTGCACA 
TATCACTATA ATGTGAATTA TCCATCGAAC ATCACAGACA AAGAACACCG CGTCCTAGTG 
AAGAAAAAGA AAGATTGTCC ACCTGCAGGT TTCCTGTGGC TCATTCCTCT CATTATGTTT 
CTGATGCTGT TACTTGGTTT GCTTTTGCTC TGTTGCTGGA AATACTGCGC CTGCTGCCAG 
TCCTGTCTTG CTCTTCTCCC GTGCTGTGCA AGAGGTCGTA TGGTGGGCTT TAAAGAAAAC 
CAGTACCTGC TACGTCAGTC TTTTCTGCAA AATGATTATT TGGACACCCC AATGGTGCGC 
AGCGGCCCGC TCAAGAGCAC AGACGTTGTG CGCTGGAAAG TGGCAGACAA TGTTCATCGA 
GGAACGAATC ACCCCCAGAA CCAAATCAGA CCCAACCCCA AAGAGACCAT CCAGTATCCT 
GTTTCTCTTC GTCTGAACCG CCAGTTTTCA GAAGTTCTTT CTAACCCTGA CGCTCGGGAC 
ACAGACATGC TCAGACGAGA AGTAGAGGAC AACCTCAATG ACGTGTTCCT GCAGATCCCG 
GGCGCACAAC CAGTGCAGAA GACTCTGTTC AGGACACAAA GAAATTCTGG AAAGAGACAG 
AACAACACAA TTGTGGACAC AGTTTTATCT GCCCCTCGCT CCAGTTACCC AGATATTGTA 
AAACTGACAG ATAAACAGGT TCAGTCTGGA AACTTCCGGG ACCTGAAGGT GATTCCAGGC 
TACTACACTG TGGCTTCTGA TAAAGAAGCA GCAGGCGCAG TGGAGTTTCC GGAGAACGTG 
GAATCAGTGG ATGTTCGGGT TCCTCTCTTC ATTAAAGATG AGGACGATGA AAAGAAGGAG 
CTGCTTGTGC AAGCTGTGGA TGTTCCAGTC GGAATCGCAA AGATTGGAAA AGATCATGTC 
AACATTACAG TGCTTAAGGA ACAGGCTTCG AGTGTCTTTA CCTTCCTGGA GCCGTCATAT 
ACCTACAGCC GTCAAGATGG AGTGGCCAAC ATCCCGATCA AACGTCAGAT CATAGAGGAT 
GGACGGGCAC AAGTCACATA CAGCACAGCA GACCTCACTG CTAAAGACAA GAAGGACTAT 
ATTTCGGTAG ATGGAGACTT GAGCTATGGA GCAGGTGAAA CAGAGAAGAT TGTCCCAGTG 
AAACTTCTGG AACTTGGAGA GCAAGATGGC CTGCTGGAGG ACAAGCAGGT CAAACAGTTT 
GTCATGGATT TGACCAACCC ACGACAAAGT GCTAAACTAG GCCGTTACCC ACGCACTGTT 
ATCACCATCG CTGACAAACC AGAATCCAGT GTTATGGCGT TTAAGAAGAG TACTCAAACT 
TTCAGTACCA CCGACTCGCT CTACACCATC CCGGTGGAAC GCACTGGAAA CCGGGAGACT 
CCGGCCACTG TGCACTGGCG CACCAACAAG GCCTCGCGCT TCGACATCTC CAGCCCTCTC 
AAATTTGCAC CCGGTGAAGC TGAAAAAAAT ATCCTCATCA ATCCTCGTAC ACATCCATCT 
CCCATCATAC CTGAGAAATT TAACCTGGAA CTATTAGACC CAAGCAACAA TGCAATTATC 
GGAAAGAGGA AGACCACGGA GGTGATCGTC ACCGACGGTC GAGGGGATGG TAAAAATCAG 
GATTTCCAGA AGATGGAATA TGTTACTCAA ACAGCCACAT CCCCCGGAGG ACGCCTCTAT 
TCTCCAGCCA ACATTAAAGC CGTTGCTACC GGCCCTAAAA ACATCCGCCT GAACTGGAAA 
CCCAGCCAAA ATGCCAATGG TTACAAGGTG AAGTACTGGA TTTATGGAGA CCCCGAAGAT 
AAAGCTAAAT TTATGGATGT GAAGACGCCT CAAGCCGAGC TGACAAATCT CGATCCATAC 
TGTGATTACG AGATGCGTGT TTGTGGCTAC AATGCACTTG GAGATGGCAA TTACAGTGAT 
ATCATCCAAT GCCAAACCCT GGAGGATATA CCTGGTGAAC CTGGTCGTCT GGCTTTCAAC 
GTCATCAGCC CTACTGTTAC TCAGGTTAGT TGGGCGGAGC CAGCAGAAGC CAATGGTATC 
ATCACTGGCT ATGAGGTCAT CTACATGCCC ATTAACGAGG ACAAGAAGCC TACAGGACCT 
CCTAAGAAGG TGATGATTGA CAACCCTAAA AAGCGCATGT TGCTCATTGA GAACCTGCAA 
CCTTCTCAGA CCTACTGTTA TAAAGTGCGT GCCAGCAATA AGGTGGGCTG GGGCCCATAC 
AGAGAAGCCA CAATCAACCT GGCATCACAG CCTACCAGAC CCATGTCAAT TCCCATCATT 
CCCGACATTC CCATCGTGGA TGCAGAAGCT GGCGAAGAGT ACGACAGCTA TCTGATGTAC 
AGTAATGAAG TCCTGCGATC CCCAACGAGC AGCCTGAGGC CTAGCGTCAC AGATCTGTCT 
GATGATCAGT TCGTGAACGG AAAGTGGGAA CAGAATTTCT TGTTTCCTGG AGGAGGAGGT 
GGTTCCCTCT CCCGCAACAT CAGCTCTTCC TCAACCACAT ACAGCCATTC CACACCGCGT 
ACCAATGTGA CCAACAACAG CTCCACCACA ACGTACATCT CTGGCAAAGG AGGCTCAACT 
ACACACAGAT ATGATGTAAG AGACGGTGGA CTTCTCAAAG AAGACGTGAT CCTGAGAAAA 
CGCTCTGAAA ACAGGCATTA CACAGAAGGT GATGGTGTGA GGGACTCAAT CGTAATGGGA 
AACCTGTCTG GTGGACTTTT GGACAATTTA GGGGTCTCTA GCTTCAGCCA GACTACATCT 
ACCAGCTACA GCTTGAGCTC CAGAGCTCGT ACTCAGTCTG AAGATATCAA CGATGCTCTG 
CTGAACCTGG ATAGTGTTCT TCAGGAATCG CGCTTCACCC CGGGTGTTCC CGCGACTCCC 
AGCAGACTGG TGTTTTCAGC TCTGGGCCAC ACGGCTCTCA AAGTGAGCTG GCAGGAGCCA 
CACTGCGAGT CTGATGTACT GGGATACTGC GTCCTTTACC AGCTCATCAA TGGAGGTGAC 
GTAAAACGTA TCGAGGTGAA CAATCCTGCT CAGAACTCAG TGCTGGTTCA GGATCTCCTG 
CCTAACCAGT CCTACATCTT CAAAGTCAAA GCACAGAGTC GAGAGGGCTG GGGTCCTGAG 
AGAGAGGGCG TCATTACCAT TGAGTCCAAC GTCGACCCCA AGAGTCCACT GAGCCCAGTG 
CCCGGGTCTC CGTTCACTCT CAGCACTCCC AGCGCTCCAG GACCTCTGAT CTTCACAGCG 
TTACGTCCAG ATACACTGCA GCTGAGCTGG GAGAAACCCC GAAAACCCAA CGGAGACATC 
CTGGGATACG TGGTGACCTG CGAACAGCTG CATGGTGGAG GAGATCTTCG CTCCTTCCAG 
GTGAATGGCA ATGCAGCCAC TTCATTGACT GTCTCTGACC TGAAAGAAAA CATGCCTTAC 
AAATTCAAAG TTCAAGCTCA GACCACGCAG GGCTTTGGCC CAGAGCGAGA GGGAATCATC 
ACCATCGAGT CCCAGGATGG AGGCTCCATG TCCCAATACA GCAGTCAGTC GGTCACGAAG 
AGAGAGGTGT TCAATCTGCC CACAGAAATC ACACACACTT CACACACCAT GTTCTCAGAG 
CCCTTTGTTT CTTCAGACGG GATGATGATG ATGTCGAGCA GGCAGATCAC AGAGTCGGGT 
GGCTCCATGA CACGGCAGGT GGAGATGGTG CAGAGAGGAG TGATGTCCAG CAGCACCGTC 
ACCAAGAGGC TGGAGAGACA GTACTACGAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
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itgb4 cDNA ORF clone, Danio rerio(zebrafish)

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ORF Insert Sequence:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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