itgb4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	itgb4
Entrez Gene ID	100485847
Full Name	integrin subunit beta 4
General protein information	Preferred Names integrin subunit beta 4 Names integrin beta-4 integrin, beta 4
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018097872.2	XP_017953361.1	integrin beta-4 isoform X1
XM_002939974.4	XP_002940020.1	integrin beta-4 isoform X1
XM_004918457.3	XP_004918514.1	integrin beta-4 isoform X2
XM_018097872.1	XP_017953361.1	integrin beta-4 isoform X1
XM_004918457.4	XP_004918514.1	integrin beta-4 isoform X2
XM_002939974.5	XP_002940020.1	integrin beta-4 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ITGB4	NP_001180186.2
Danio rerio (zebrafish)	itgb4	NP_001019557.1
Pan troglodytes (chimpanzee)	ITGB4	XP_003315785.1
Rattus norvegicus (Norway rat)	Itgb4	NP_037312.1
Xenopus tropicalis (tropical clawed frog)	itgb4	XP_004918514.1
Canis lupus familiaris (dog)	ITGB4	XP_005624218.1
Gallus gallus (chicken)	ITGB4	XP_003642374.2
Homo sapiens (human)	ITGB4	NP_000204.3
	ITGB4	XP_002800666.1
Mus musculus (house mouse)	Itgb4	NP_001005608.2

Gene Ontology

The following itgb4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the itgb4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa50383	XM_018097872.2 Latest version!	Xenopus tropicalis integrin subunit beta 4 (itgb4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa50383	XM_002939974.5 Latest version!	Xenopus tropicalis integrin subunit beta 4 (itgb4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa50384	XM_004918457.4 Latest version!	Xenopus tropicalis integrin subunit beta 4 (itgb4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa17190	XM_002939974.4 Latest version!	Xenopus tropicalis integrin subunit beta 4 (itgb4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa17190	XM_018097872.1 Latest version!	Xenopus tropicalis integrin subunit beta 4 (itgb4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa17191	XM_004918457.3 Latest version!	Xenopus tropicalis integrin subunit beta 4 (itgb4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

itgb4 ( XM_018097872.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017953361.1 Xenopus tropicalis integrin subunit beta 4 (itgb4), transcript variant X2, mRNA.

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CloneID	OXa50383
Clone ID Related Accession (Same CDS sequence)	XM_018097872.2 , XM_002939974.5
Accession Version	XM_018097872.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	integrin beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018097872.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAACC CTCGTGTTTT AGGGGGTCTC CTCCTGCTTG TCCTGCTCTT GGGTCTCCCA 
GCTTCCCATT CTCAGAGCAA CAAATATAAC CCCTGCGTGG CCTCCAGAGC AACCAGTTGC 
TCGGCCTGTA TCCGGGCGTA CAAGGACTGT TCCTACTGTG CAGATGAGGG TTTCGCTAGT 
GTACGCTGTG ATCTAGCAGA GAATCTGAGA AGATATGGCT GCAGCGAGAG AGGGATTGTT 
TACATGAGAA GTGAAGTCTT GACCCAGCAG AGCTATGACA TTAACACTCT CGTCAGCAAA 
ACCCAAGTGG CCCCCCAAAG GATGTCCATG CGTCTTCGTG CTGGGGAAGA AACCTCATTT 
GACCTGAATG TGTTTGAGCC GCTGGACACC CCTGTGGATT TGTACATTTT GATGGACTTC 
TCTCATTCCA TGTCAGATGA TTTAGCCAAC CTAAAAAATA TGGGTCAGGA GTTAGCTAAT 
GTTGTAAGCA CTCTCTCCAA GAATTACACC ATTGGATTTG GAAAATTTGT GGACAAAGTG 
ACTGTACCAC AGACTGACAT GAGACCTGAG AAACTGAAGC AACCCTGGTT GGACAGCACT 
CCTCCTTTCT CCTTCAAGAA CGTTATTCAA CTGACAAGCG ACGTGGGGAA ATTTCGGAAT 
GAATTGATGA AAGAGAAAAT CTCTGGAAAC CTTGATCCTC CAGAGGGGGG GTTTGATGCC 
ATTCTTCAGA CTGCTGTGTG CACGAATCAA ATTGGCTGGA GAAAAGGAAG TACTCATCTG 
TTGGTCTTCT CTACTGAGTC AGCATTCCAT TATGAAGCTG ATGGGATGAA CGTACTTGAT 
GGGATACTGA AGCGAAATGA TGAGCAGTGC CACTTGGATG CCTCTGGTTA CTACACACAC 
GACATCGTCC AGGACTATCC ATCTGTGCCC ACACTTGTTC GACTGTTAGC AAAGAACAAC 
ATCATCCCTA TCTTTGCCGT CACCAACTAC TCCTACAGTT ACTATGATAA GCTGAGCAAG 
TATTTCAAAG TATCTGAGAT TGGAGAATTG AAAGAAGATT CCTCCAACAT TGTGGAGCTC 
TTGCAGGAGG CCTTTACGCA AATTCGTTCC AGGATGGATA TCCGAGATGA TAACACCCCA 
CGTGCAATGG ATGTCTCAGT CACTTCTGAG ACAGCTAAAG TTACAGAAAC AGGATCGTTT 
CAAGTCACCC GGGGAGAAGT GGGTACCTTC AAAGTGAAGG TCAAGGCTTT AGAACGGTTG 
GGCAACCAGC ATGTGTGTGA ACTACCACCA CAAGACCAAG GGAGCCAAAT TCTCCTCAAA 
CCTTCCACCT TTACTGATGG CCTTAAGATT GATGCTTCTC TGATCTGTGA CAGCTGTCCT 
TGTGAATTGC AAAAGGAGTT GAGTTCCCCC AAGTGCAGCT TTAATGGCGA CTTTGTGTGC 
GGACGCTGTG AGTGCAGGGA GCGCTGGAGG GGAGTATATT GTAACTGCTC CAGCACAGAC 
ACCAAAAGCA CCCAATCGTG TATATCACCA GGCAGTACAG AGATTTGCTC CGGCAGGGGT 
GAATGCCTAT GCGGCACCTG TCAGTGTCAC TCGGAGTCCC CCACTCAACA GTACCAGGGG 
GAGTTCTGCC AGTTCAATAA CTTCCAGTGC CCACGTTTTC TTGGATACAT GTGCAATGAC 
AGAGGGCGCT GTCACATGGG AGCCTGTGTG TGTGATGCTG GCTGGGAAGG AGAGAGTTGT 
CAGTGTCCTA CCAGCAACCA AACCTGCCTT GATAGGAATG GGGGTCTGTG CAGTAGCCGT 
GGGAAGTGCG TGTGTGGGCG CTGCCAGTGT GATAACGTGT CACAGTATAC AGACGCCACG 
TGTGAATTCT CCTATTCACT GCGCCAACTG GGTGTGTGCG AAGACCTGCG GACGTGTGTG 
CAGTGCCAGG CCTGGGGAAC TGGAGAGAAA AAGGGCACAC AATGTGAAGC TTGTGAAATT 
AAGATTAAAA TGGTGGATGA GCTGAAGAAA GTGGATGAAA GAAGTGAAAA CTGTACTTTC 
CGGGATGAAA CAGATGACTG TACATATGAG TACTCAGTAG ACCCTCACGC AGTGCAAGGA 
AACTACACTG TGCTGGTCAA GAACAAAAAA GAATGTCCTC CTGGAGCCTT CCTCTGGCTT 
ATCCCTCTAC TCATCTTTCT GATGCTTCTC CTTGGGCTCT TGTTGCTCCT GTGCTGGAAA 
TACTGTGCCT GTTGCAAGGC TTGCTTGGCA ATGCTTCCCT GCTGTGGACC AGGACGGACA 
GTGGGATTTA AAGAAGACCA CTACATGTTA AGGCAAAGCC TGATGTCATC TGATTACCTG 
AATACCCCAC TGGTGCGTTC TGGGAGCCTC AAGGGCGGGG ACACTGTAAG ATGGAAGATC 
GACAACAACG TGCACCGCCA ACCACCCGCC CCTGGAACAC TGAACCCCAA GGAGCTTGTC 
CCATATGGCG TCTCACTGCG TCTGGCCCGG CTGTTCACTG AGAACCTGTC CAAGCCTCAA 
TCCCGTGAGT GTACCCAGCT GCGTCAGGAG GTGGAAGATT CGTTAAATGA GGTGTACAAG 
GTGATACCTG GAACCCACAA GGTGAAACAG ACAGCTTTCA GGCTTCAGTC CAATGCAGGA 
AAAAGACAGG ATCACACTAT TGCAGAAACC ATCCTGATGG CTCCCCGGGA GGCTGAACCT 
GAGATTGTAA AGGTTACAGA GAAACATGTA ACACAAGAGG CTTTCCAGGA TATGAAGGTT 
GCCCCAGGTT ACTATACAGT GACGTCAGAC AGAGATGCCC ATGGACTTGT AGAGTTCCAG 
GAGGGAGTTG AATTGGTGGA TGTCCGGGTT CCTCTCTTCA TCAGGGAAGA GGATGATGAT 
GAGAAGCAGT TGTTAGTGGA AGCTCTGGAC GTTCCATCAG GAATTGCCCG TTTGGGGCGG 
AAACTGGTCA ATATTACTAT TATCAAAGAG CAAGCCAAGA GCATAGTGAC GTTTGTGAAA 
CCTGCCTACA CCTACAGCCG ACAGGAACAG GTTGCCCGTG TCCCAGTTTC TCGTGAGATC 
ATCCAGAATG GAAAGACACA AGTTACATAT CGTTCCAAGG ACCTGACTGC TAGGGAGGGA 
AAGGACTATA CATTCTCAGA GGGGGAGCTC ACCTTCCAGC CAGATGACAC TCAATCAGAG 
ATTCTAGTGC CTATACTCGG TCGTTCAGAA GTTGACACTC TGCTGGGAAA TAAACAAGTC 
AAACAGTTCC TTCTAGAACT CAGTAATCCT AAGTATGGGG CCAAGATTGG CAAATACCCA 
CAATGCACCA TCACTATTGA TGACACTGCA GATGTTATAC AAAAGACAAT GACAGTCCAG 
TCTGTTCAGT CCCCCCGTGG GAAGGTAGGT GCTCCTATCA ACCTGAATGC CCAGGCCCTC 
AGCGCCAGGA AAATCCGACT CAACTGGATG CCGCCTCCTG GAAAACCCAC AGGTTACAAG 
GTGCGTTACT GGATTCAAGG AGATTCCGAG TCAGATGCGA CTGTGGTGGA TACAAATGTG 
CCCTCTGCAG AGCTGGTCAA TCTGTACCCC TACTGTGACT ACGAGATGCG GGTATGCGCA 
TACAATGCTC AAGAGGAGGG TCCCTTCACT GACATAGTCA CCTGCCAGAC CTTGGAGGAA 
GCACCCAGTG AACCCGGCCG CCTCGCCTTT AACGTCATTT CAAGCACAGT GACGCAGCTG 
AGTTGGGCAG AGCCAGCTGA AACCAATGGT GAAATCACAG CGTATGAGGT CACCTACGGA 
ATGGTCAACG AGGACAACAA ACCCATTGGG CCCACGAAGA AAGTCCTGAT TAATGATTCA 
AAGAAGCGCA TGGTTCTAAT TGAAAACCTA CGAGAATCCC AGCCATACCG ATACACTGTG 
AGAGCCTGCA ACAAAGCTGG GTGGGGGCCA GAGAGAGATG CCACCATGAA CCTAGCTACC 
CAACCCACCC GCCCAATGTC TATTCCCATT ATCCCTGATG CTCCCATTAT TGATGCTGAA 
GCTGGGGAAG AATATGACAG TTACCTGATG TACAGCACCG ATGTCCTGCG CTCTCCAGTT 
GGCAGCAAGA GACCCAGTGT GTCCGACGAG TCAGGCCAGC GCTGGAAGTA TGTCCCTCTC 
CTGGGCTCGG AGATGGACCT GCGGCGGGTC ACCTGGAAGC TACCCGTAGA GGTCATACCA 
CGCCTGTCCG TCAGCAGCGG CCTGTCCACT GACAACGAGA GCTTGCTGGA CCAGCCTTTG 
ACTACCAGCT CACTGACGAG GAAAGGGACC ATGCGAGCAA CCCCAGAAGA GCAATTAATT 
AACGGACGCA CAGAATTCTC CTTCTCGGGC GGCAGTCTCA ACCGTTCCAT GGTATCAGGG 
TACCAGCAAC TCAGCCCGCA CGTGCAAAAT GAAAGCTACA GGTTTGGTAG CAGCAGTTCC 
CAGCATACAT CCACAACGGT CATATCAGGG CAAGGCTCAT CAGGGCAGAA CAGACTGGGG 
CTCCCCCCAT TGGTGTTAGG AGGAGGAAGG GGACGAACCC ACTCAGAGGA TGTGAGGGAT 
GCTCTGACAA GCTTGGATGT GGCCATACAG GATACACGTC TCTCCCCGGG AATCCCGGAT 
ACCCCTACCC GTCTGGTATT CTCAGCTCTG GGACCCACCT CCCTAAAGGT CAGTTGGCAG 
GAGCCGCAAT GCGAAAGAGA AGTTCTGGGA TACAAAGTGC ATTATCAACT ACTGCATGGA 
GGTGAATTAA GGACCATCGA CATTACAAAC CCACGGGAGA ACTCAGTAGT GGTGGAAGAC 
TTGCTTCCTA ACCACTCGTA CATGTTCCGT GTGAAGGCAC AAAGCGTTGA GGGCTGGGGG 
CCCGAAAGAG AAGGCGTTAT CACTATCGAA TCACAGGTGG ATCCTCAGAG TCCACTCAGC 
CCTGTTCCAG GGTCTCCATT CACCCTAAGC ACCCCCAGTG CTCCTGGACC ACTGGTTTTC 
ACAGCTCTGG CCCCTGACTC CCTGCAGTTA AGTTGGGTGA GACCCCGAAA GCCTAATGGA 
ACAATTCTGG GGTACATGAT CACTTGCGAG ATGTTGCATG GAGGAGGGGA ACCACGAACG 
ATCTATGTTG AAGGTGACAA ACCCGAGACT TCCCTGACGG TTCCTTTCCT AAATGAAAAT 
CTGCCCTATA AATTTAAGGT CCAGGCGAAA ACCACACAGG GTTTTGGACC AGAACGGGAA 
GGGATAATTA CTATTGAATC TCAAGATGGA GGAAACTTTT CTCAGTTTGG AACACAACAG 
ATCACACGCC GAGAGGTGTT CAATATGCCA GGAGAATATA CTCAAAGCAG CATCACTCAA 
TCCAACATAA CAGAACCCTT CTTTTCAGAT GGAATGAGTG TCACCACCCG CCGTATCGAG 
GGCTCCAACA CAGTCACACA ACAGGTCACC AAAGAATTTG TCACACGGAC CATGATGTCT 
GGCGGCTCCC TCAACAGGCA AGTGGAGCGC CAGTTTTACG ACGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017953361.1
CDS	588..6095
Translation	MMNPRVLGGLLLLVLLLGLPASHSQSNKYNPCVASRATSCSACIRAYKDCSYCADEGFASVRCDLAENLRRYGCSERGIVYMRSEVLTQQSYDINTLVSKTQVAPQRMSMRLRAGEETSFDLNVFEPLDTPVDLYILMDFSHSMSDDLANLKNMGQELANVVSTLSKNYTIGFGKFVDKVTVPQTDMRPEKLKQPWLDSTPPFSFKNVIQLTSDVGKFRNELMKEKISGNLDPPEGGFDAILQTAVCTNQIGWRKGSTHLLVFSTESAFHYEADGMNVLDGILKRNDEQCHLDASGYYTHDIVQDYPSVPTLVRLLAKNNIIPIFAVTNYSYSYYDKLSKYFKVSEIGELKEDSSNIVELLQEAFTQIRSRMDIRDDNTPRAMDVSVTSETAKVTETGSFQVTRGEVGTFKVKVKALERLGNQHVCELPPQDQGSQILLKPSTFTDGLKIDASLICDSCPCELQKELSSPKCSFNGDFVCGRCECRERWRGVYCNCSSTDTKSTQSCISPGSTEICSGRGECLCGTCQCHSESPTQQYQGEFCQFNNFQCPRFLGYMCNDRGRCHMGACVCDAGWEGESCQCPTSNQTCLDRNGGLCSSRGKCVCGRCQCDNVSQYTDATCEFSYSLRQLGVCEDLRTCVQCQAWGTGEKKGTQCEACEIKIKMVDELKKVDERSENCTFRDETDDCTYEYSVDPHAVQGNYTVLVKNKKECPPGAFLWLIPLLIFLMLLLGLLLLLCWKYCACCKACLAMLPCCGPGRTVGFKEDHYMLRQSLMSSDYLNTPLVRSGSLKGGDTVRWKIDNNVHRQPPAPGTLNPKELVPYGVSLRLARLFTENLSKPQSRECTQLRQEVEDSLNEVYKVIPGTHKVKQTAFRLQSNAGKRQDHTIAETILMAPREAEPEIVKVTEKHVTQEAFQDMKVAPGYYTVTSDRDAHGLVEFQEGVELVDVRVPLFIREEDDDEKQLLVEALDVPSGIARLGRKLVNITIIKEQAKSIVTFVKPAYTYSRQEQVARVPVSREIIQNGKTQVTYRSKDLTAREGKDYTFSEGELTFQPDDTQSEILVPILGRSEVDTLLGNKQVKQFLLELSNPKYGAKIGKYPQCTITIDDTADVIQKTMTVQSVQSPRGKVGAPINLNAQALSARKIRLNWMPPPGKPTGYKVRYWIQGDSESDATVVDTNVPSAELVNLYPYCDYEMRVCAYNAQEEGPFTDIVTCQTLEEAPSEPGRLAFNVISSTVTQLSWAEPAETNGEITAYEVTYGMVNEDNKPIGPTKKVLINDSKKRMVLIENLRESQPYRYTVRACNKAGWGPERDATMNLATQPTRPMSIPIIPDAPIIDAEAGEEYDSYLMYSTDVLRSPVGSKRPSVSDESGQRWKYVPLLGSEMDLRRVTWKLPVEVIPRLSVSSGLSTDNESLLDQPLTTSSLTRKGTMRATPEEQLINGRTEFSFSGGSLNRSMVSGYQQLSPHVQNESYRFGSSSSQHTSTTVISGQGSSGQNRLGLPPLVLGGGRGRTHSEDVRDALTSLDVAIQDTRLSPGIPDTPTRLVFSALGPTSLKVSWQEPQCEREVLGYKVHYQLLHGGELRTIDITNPRENSVVVEDLLPNHSYMFRVKAQSVEGWGPEREGVITIESQVDPQSPLSPVPGSPFTLSTPSAPGPLVFTALAPDSLQLSWVRPRKPNGTILGYMITCEMLHGGGEPRTIYVEGDKPETSLTVPFLNENLPYKFKVQAKTTQGFGPEREGIITIESQDGGNFSQFGTQQITRREVFNMPGEYTQSSITQSNITEPFFSDGMSVTTRRIEGSNTVTQQVTKEFVTRTMMSGGSLNRQVERQFYDA

Target ORF information:

RefSeq Version	XM_018097872.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis integrin subunit beta 4 (itgb4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018097872.2

ORF Insert Sequence:

ATGATGAACC CTCGTGTTTT AGGGGGTCTC CTCCTGCTTG TCCTGCTCTT GGGTCTCCCA 
GCTTCCCATT CTCAGAGCAA CAAATATAAC CCCTGCGTGG CCTCCAGAGC AACCAGTTGC 
TCGGCCTGTA TCCGGGCGTA CAAGGACTGT TCCTACTGTG CAGATGAGGG TTTCGCTAGT 
GTACGCTGTG ATCTAGCAGA GAATCTGAGA AGATATGGCT GCAGCGAGAG AGGGATTGTT 
TACATGAGAA GTGAAGTCTT GACCCAGCAG AGCTATGACA TTAACACTCT CGTCAGCAAA 
ACCCAAGTGG CCCCCCAAAG GATGTCCATG CGTCTTCGTG CTGGGGAAGA AACCTCATTT 
GACCTGAATG TGTTTGAGCC GCTGGACACC CCTGTGGATT TGTACATTTT GATGGACTTC 
TCTCATTCCA TGTCAGATGA TTTAGCCAAC CTAAAAAATA TGGGTCAGGA GTTAGCTAAT 
GTTGTAAGCA CTCTCTCCAA GAATTACACC ATTGGATTTG GAAAATTTGT GGACAAAGTG 
ACTGTACCAC AGACTGACAT GAGACCTGAG AAACTGAAGC AACCCTGGTT GGACAGCACT 
CCTCCTTTCT CCTTCAAGAA CGTTATTCAA CTGACAAGCG ACGTGGGGAA ATTTCGGAAT 
GAATTGATGA AAGAGAAAAT CTCTGGAAAC CTTGATCCTC CAGAGGGGGG GTTTGATGCC 
ATTCTTCAGA CTGCTGTGTG CACGAATCAA ATTGGCTGGA GAAAAGGAAG TACTCATCTG 
TTGGTCTTCT CTACTGAGTC AGCATTCCAT TATGAAGCTG ATGGGATGAA CGTACTTGAT 
GGGATACTGA AGCGAAATGA TGAGCAGTGC CACTTGGATG CCTCTGGTTA CTACACACAC 
GACATCGTCC AGGACTATCC ATCTGTGCCC ACACTTGTTC GACTGTTAGC AAAGAACAAC 
ATCATCCCTA TCTTTGCCGT CACCAACTAC TCCTACAGTT ACTATGATAA GCTGAGCAAG 
TATTTCAAAG TATCTGAGAT TGGAGAATTG AAAGAAGATT CCTCCAACAT TGTGGAGCTC 
TTGCAGGAGG CCTTTACGCA AATTCGTTCC AGGATGGATA TCCGAGATGA TAACACCCCA 
CGTGCAATGG ATGTCTCAGT CACTTCTGAG ACAGCTAAAG TTACAGAAAC AGGATCGTTT 
CAAGTCACCC GGGGAGAAGT GGGTACCTTC AAAGTGAAGG TCAAGGCTTT AGAACGGTTG 
GGCAACCAGC ATGTGTGTGA ACTACCACCA CAAGACCAAG GGAGCCAAAT TCTCCTCAAA 
CCTTCCACCT TTACTGATGG CCTTAAGATT GATGCTTCTC TGATCTGTGA CAGCTGTCCT 
TGTGAATTGC AAAAGGAGTT GAGTTCCCCC AAGTGCAGCT TTAATGGCGA CTTTGTGTGC 
GGACGCTGTG AGTGCAGGGA GCGCTGGAGG GGAGTATATT GTAACTGCTC CAGCACAGAC 
ACCAAAAGCA CCCAATCGTG TATATCACCA GGCAGTACAG AGATTTGCTC CGGCAGGGGT 
GAATGCCTAT GCGGCACCTG TCAGTGTCAC TCGGAGTCCC CCACTCAACA GTACCAGGGG 
GAGTTCTGCC AGTTCAATAA CTTCCAGTGC CCACGTTTTC TTGGATACAT GTGCAATGAC 
AGAGGGCGCT GTCACATGGG AGCCTGTGTG TGTGATGCTG GCTGGGAAGG AGAGAGTTGT 
CAGTGTCCTA CCAGCAACCA AACCTGCCTT GATAGGAATG GGGGTCTGTG CAGTAGCCGT 
GGGAAGTGCG TGTGTGGGCG CTGCCAGTGT GATAACGTGT CACAGTATAC AGACGCCACG 
TGTGAATTCT CCTATTCACT GCGCCAACTG GGTGTGTGCG AAGACCTGCG GACGTGTGTG 
CAGTGCCAGG CCTGGGGAAC TGGAGAGAAA AAGGGCACAC AATGTGAAGC TTGTGAAATT 
AAGATTAAAA TGGTGGATGA GCTGAAGAAA GTGGATGAAA GAAGTGAAAA CTGTACTTTC 
CGGGATGAAA CAGATGACTG TACATATGAG TACTCAGTAG ACCCTCACGC AGTGCAAGGA 
AACTACACTG TGCTGGTCAA GAACAAAAAA GAATGTCCTC CTGGAGCCTT CCTCTGGCTT 
ATCCCTCTAC TCATCTTTCT GATGCTTCTC CTTGGGCTCT TGTTGCTCCT GTGCTGGAAA 
TACTGTGCCT GTTGCAAGGC TTGCTTGGCA ATGCTTCCCT GCTGTGGACC AGGACGGACA 
GTGGGATTTA AAGAAGACCA CTACATGTTA AGGCAAAGCC TGATGTCATC TGATTACCTG 
AATACCCCAC TGGTGCGTTC TGGGAGCCTC AAGGGCGGGG ACACTGTAAG ATGGAAGATC 
GACAACAACG TGCACCGCCA ACCACCCGCC CCTGGAACAC TGAACCCCAA GGAGCTTGTC 
CCATATGGCG TCTCACTGCG TCTGGCCCGG CTGTTCACTG AGAACCTGTC CAAGCCTCAA 
TCCCGTGAGT GTACCCAGCT GCGTCAGGAG GTGGAAGATT CGTTAAATGA GGTGTACAAG 
GTGATACCTG GAACCCACAA GGTGAAACAG ACAGCTTTCA GGCTTCAGTC CAATGCAGGA 
AAAAGACAGG ATCACACTAT TGCAGAAACC ATCCTGATGG CTCCCCGGGA GGCTGAACCT 
GAGATTGTAA AGGTTACAGA GAAACATGTA ACACAAGAGG CTTTCCAGGA TATGAAGGTT 
GCCCCAGGTT ACTATACAGT GACGTCAGAC AGAGATGCCC ATGGACTTGT AGAGTTCCAG 
GAGGGAGTTG AATTGGTGGA TGTCCGGGTT CCTCTCTTCA TCAGGGAAGA GGATGATGAT 
GAGAAGCAGT TGTTAGTGGA AGCTCTGGAC GTTCCATCAG GAATTGCCCG TTTGGGGCGG 
AAACTGGTCA ATATTACTAT TATCAAAGAG CAAGCCAAGA GCATAGTGAC GTTTGTGAAA 
CCTGCCTACA CCTACAGCCG ACAGGAACAG GTTGCCCGTG TCCCAGTTTC TCGTGAGATC 
ATCCAGAATG GAAAGACACA AGTTACATAT CGTTCCAAGG ACCTGACTGC TAGGGAGGGA 
AAGGACTATA CATTCTCAGA GGGGGAGCTC ACCTTCCAGC CAGATGACAC TCAATCAGAG 
ATTCTAGTGC CTATACTCGG TCGTTCAGAA GTTGACACTC TGCTGGGAAA TAAACAAGTC 
AAACAGTTCC TTCTAGAACT CAGTAATCCT AAGTATGGGG CCAAGATTGG CAAATACCCA 
CAATGCACCA TCACTATTGA TGACACTGCA GATGTTATAC AAAAGACAAT GACAGTCCAG 
TCTGTTCAGT CCCCCCGTGG GAAGGTAGGT GCTCCTATCA ACCTGAATGC CCAGGCCCTC 
AGCGCCAGGA AAATCCGACT CAACTGGATG CCGCCTCCTG GAAAACCCAC AGGTTACAAG 
GTGCGTTACT GGATTCAAGG AGATTCCGAG TCAGATGCGA CTGTGGTGGA TACAAATGTG 
CCCTCTGCAG AGCTGGTCAA TCTGTACCCC TACTGTGACT ACGAGATGCG GGTATGCGCA 
TACAATGCTC AAGAGGAGGG TCCCTTCACT GACATAGTCA CCTGCCAGAC CTTGGAGGAA 
GCACCCAGTG AACCCGGCCG CCTCGCCTTT AACGTCATTT CAAGCACAGT GACGCAGCTG 
AGTTGGGCAG AGCCAGCTGA AACCAATGGT GAAATCACAG CGTATGAGGT CACCTACGGA 
ATGGTCAACG AGGACAACAA ACCCATTGGG CCCACGAAGA AAGTCCTGAT TAATGATTCA 
AAGAAGCGCA TGGTTCTAAT TGAAAACCTA CGAGAATCCC AGCCATACCG ATACACTGTG 
AGAGCCTGCA ACAAAGCTGG GTGGGGGCCA GAGAGAGATG CCACCATGAA CCTAGCTACC 
CAACCCACCC GCCCAATGTC TATTCCCATT ATCCCTGATG CTCCCATTAT TGATGCTGAA 
GCTGGGGAAG AATATGACAG TTACCTGATG TACAGCACCG ATGTCCTGCG CTCTCCAGTT 
GGCAGCAAGA GACCCAGTGT GTCCGACGAG TCAGGCCAGC GCTGGAAGTA TGTCCCTCTC 
CTGGGCTCGG AGATGGACCT GCGGCGGGTC ACCTGGAAGC TACCCGTAGA GGTCATACCA 
CGCCTGTCCG TCAGCAGCGG CCTGTCCACT GACAACGAGA GCTTGCTGGA CCAGCCTTTG 
ACTACCAGCT CACTGACGAG GAAAGGGACC ATGCGAGCAA CCCCAGAAGA GCAATTAATT 
AACGGACGCA CAGAATTCTC CTTCTCGGGC GGCAGTCTCA ACCGTTCCAT GGTATCAGGG 
TACCAGCAAC TCAGCCCGCA CGTGCAAAAT GAAAGCTACA GGTTTGGTAG CAGCAGTTCC 
CAGCATACAT CCACAACGGT CATATCAGGG CAAGGCTCAT CAGGGCAGAA CAGACTGGGG 
CTCCCCCCAT TGGTGTTAGG AGGAGGAAGG GGACGAACCC ACTCAGAGGA TGTGAGGGAT 
GCTCTGACAA GCTTGGATGT GGCCATACAG GATACACGTC TCTCCCCGGG AATCCCGGAT 
ACCCCTACCC GTCTGGTATT CTCAGCTCTG GGACCCACCT CCCTAAAGGT CAGTTGGCAG 
GAGCCGCAAT GCGAAAGAGA AGTTCTGGGA TACAAAGTGC ATTATCAACT ACTGCATGGA 
GGTGAATTAA GGACCATCGA CATTACAAAC CCACGGGAGA ACTCAGTAGT GGTGGAAGAC 
TTGCTTCCTA ACCACTCGTA CATGTTCCGT GTGAAGGCAC AAAGCGTTGA GGGCTGGGGG 
CCCGAAAGAG AAGGCGTTAT CACTATCGAA TCACAGGTGG ATCCTCAGAG TCCACTCAGC 
CCTGTTCCAG GGTCTCCATT CACCCTAAGC ACCCCCAGTG CTCCTGGACC ACTGGTTTTC 
ACAGCTCTGG CCCCTGACTC CCTGCAGTTA AGTTGGGTGA GACCCCGAAA GCCTAATGGA 
ACAATTCTGG GGTACATGAT CACTTGCGAG ATGTTGCATG GAGGAGGGGA ACCACGAACG 
ATCTATGTTG AAGGTGACAA ACCCGAGACT TCCCTGACGG TTCCTTTCCT AAATGAAAAT 
CTGCCCTATA AATTTAAGGT CCAGGCGAAA ACCACACAGG GTTTTGGACC AGAACGGGAA 
GGGATAATTA CTATTGAATC TCAAGATGGA GGAAACTTTT CTCAGTTTGG AACACAACAG 
ATCACACGCC GAGAGGTGTT CAATATGCCA GGAGAATATA CTCAAAGCAG CATCACTCAA 
TCCAACATAA CAGAACCCTT CTTTTCAGAT GGAATGAGTG TCACCACCCG CCGTATCGAG 
GGCTCCAACA CAGTCACACA ACAGGTCACC AAAGAATTTG TCACACGGAC CATGATGTCT 
GGCGGCTCCC TCAACAGGCA AGTGGAGCGC CAGTTTTACG ACGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itgb4 ( XM_002939974.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940020.1 Xenopus tropicalis integrin subunit beta 4 (itgb4), transcript variant X1, mRNA.

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CloneID	OXa50383
Clone ID Related Accession (Same CDS sequence)	XM_018097872.2 , XM_002939974.5
Accession Version	XM_002939974.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	integrin beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002939974.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAACC CTCGTGTTTT AGGGGGTCTC CTCCTGCTTG TCCTGCTCTT GGGTCTCCCA 
GCTTCCCATT CTCAGAGCAA CAAATATAAC CCCTGCGTGG CCTCCAGAGC AACCAGTTGC 
TCGGCCTGTA TCCGGGCGTA CAAGGACTGT TCCTACTGTG CAGATGAGGG TTTCGCTAGT 
GTACGCTGTG ATCTAGCAGA GAATCTGAGA AGATATGGCT GCAGCGAGAG AGGGATTGTT 
TACATGAGAA GTGAAGTCTT GACCCAGCAG AGCTATGACA TTAACACTCT CGTCAGCAAA 
ACCCAAGTGG CCCCCCAAAG GATGTCCATG CGTCTTCGTG CTGGGGAAGA AACCTCATTT 
GACCTGAATG TGTTTGAGCC GCTGGACACC CCTGTGGATT TGTACATTTT GATGGACTTC 
TCTCATTCCA TGTCAGATGA TTTAGCCAAC CTAAAAAATA TGGGTCAGGA GTTAGCTAAT 
GTTGTAAGCA CTCTCTCCAA GAATTACACC ATTGGATTTG GAAAATTTGT GGACAAAGTG 
ACTGTACCAC AGACTGACAT GAGACCTGAG AAACTGAAGC AACCCTGGTT GGACAGCACT 
CCTCCTTTCT CCTTCAAGAA CGTTATTCAA CTGACAAGCG ACGTGGGGAA ATTTCGGAAT 
GAATTGATGA AAGAGAAAAT CTCTGGAAAC CTTGATCCTC CAGAGGGGGG GTTTGATGCC 
ATTCTTCAGA CTGCTGTGTG CACGAATCAA ATTGGCTGGA GAAAAGGAAG TACTCATCTG 
TTGGTCTTCT CTACTGAGTC AGCATTCCAT TATGAAGCTG ATGGGATGAA CGTACTTGAT 
GGGATACTGA AGCGAAATGA TGAGCAGTGC CACTTGGATG CCTCTGGTTA CTACACACAC 
GACATCGTCC AGGACTATCC ATCTGTGCCC ACACTTGTTC GACTGTTAGC AAAGAACAAC 
ATCATCCCTA TCTTTGCCGT CACCAACTAC TCCTACAGTT ACTATGATAA GCTGAGCAAG 
TATTTCAAAG TATCTGAGAT TGGAGAATTG AAAGAAGATT CCTCCAACAT TGTGGAGCTC 
TTGCAGGAGG CCTTTACGCA AATTCGTTCC AGGATGGATA TCCGAGATGA TAACACCCCA 
CGTGCAATGG ATGTCTCAGT CACTTCTGAG ACAGCTAAAG TTACAGAAAC AGGATCGTTT 
CAAGTCACCC GGGGAGAAGT GGGTACCTTC AAAGTGAAGG TCAAGGCTTT AGAACGGTTG 
GGCAACCAGC ATGTGTGTGA ACTACCACCA CAAGACCAAG GGAGCCAAAT TCTCCTCAAA 
CCTTCCACCT TTACTGATGG CCTTAAGATT GATGCTTCTC TGATCTGTGA CAGCTGTCCT 
TGTGAATTGC AAAAGGAGTT GAGTTCCCCC AAGTGCAGCT TTAATGGCGA CTTTGTGTGC 
GGACGCTGTG AGTGCAGGGA GCGCTGGAGG GGAGTATATT GTAACTGCTC CAGCACAGAC 
ACCAAAAGCA CCCAATCGTG TATATCACCA GGCAGTACAG AGATTTGCTC CGGCAGGGGT 
GAATGCCTAT GCGGCACCTG TCAGTGTCAC TCGGAGTCCC CCACTCAACA GTACCAGGGG 
GAGTTCTGCC AGTTCAATAA CTTCCAGTGC CCACGTTTTC TTGGATACAT GTGCAATGAC 
AGAGGGCGCT GTCACATGGG AGCCTGTGTG TGTGATGCTG GCTGGGAAGG AGAGAGTTGT 
CAGTGTCCTA CCAGCAACCA AACCTGCCTT GATAGGAATG GGGGTCTGTG CAGTAGCCGT 
GGGAAGTGCG TGTGTGGGCG CTGCCAGTGT GATAACGTGT CACAGTATAC AGACGCCACG 
TGTGAATTCT CCTATTCACT GCGCCAACTG GGTGTGTGCG AAGACCTGCG GACGTGTGTG 
CAGTGCCAGG CCTGGGGAAC TGGAGAGAAA AAGGGCACAC AATGTGAAGC TTGTGAAATT 
AAGATTAAAA TGGTGGATGA GCTGAAGAAA GTGGATGAAA GAAGTGAAAA CTGTACTTTC 
CGGGATGAAA CAGATGACTG TACATATGAG TACTCAGTAG ACCCTCACGC AGTGCAAGGA 
AACTACACTG TGCTGGTCAA GAACAAAAAA GAATGTCCTC CTGGAGCCTT CCTCTGGCTT 
ATCCCTCTAC TCATCTTTCT GATGCTTCTC CTTGGGCTCT TGTTGCTCCT GTGCTGGAAA 
TACTGTGCCT GTTGCAAGGC TTGCTTGGCA ATGCTTCCCT GCTGTGGACC AGGACGGACA 
GTGGGATTTA AAGAAGACCA CTACATGTTA AGGCAAAGCC TGATGTCATC TGATTACCTG 
AATACCCCAC TGGTGCGTTC TGGGAGCCTC AAGGGCGGGG ACACTGTAAG ATGGAAGATC 
GACAACAACG TGCACCGCCA ACCACCCGCC CCTGGAACAC TGAACCCCAA GGAGCTTGTC 
CCATATGGCG TCTCACTGCG TCTGGCCCGG CTGTTCACTG AGAACCTGTC CAAGCCTCAA 
TCCCGTGAGT GTACCCAGCT GCGTCAGGAG GTGGAAGATT CGTTAAATGA GGTGTACAAG 
GTGATACCTG GAACCCACAA GGTGAAACAG ACAGCTTTCA GGCTTCAGTC CAATGCAGGA 
AAAAGACAGG ATCACACTAT TGCAGAAACC ATCCTGATGG CTCCCCGGGA GGCTGAACCT 
GAGATTGTAA AGGTTACAGA GAAACATGTA ACACAAGAGG CTTTCCAGGA TATGAAGGTT 
GCCCCAGGTT ACTATACAGT GACGTCAGAC AGAGATGCCC ATGGACTTGT AGAGTTCCAG 
GAGGGAGTTG AATTGGTGGA TGTCCGGGTT CCTCTCTTCA TCAGGGAAGA GGATGATGAT 
GAGAAGCAGT TGTTAGTGGA AGCTCTGGAC GTTCCATCAG GAATTGCCCG TTTGGGGCGG 
AAACTGGTCA ATATTACTAT TATCAAAGAG CAAGCCAAGA GCATAGTGAC GTTTGTGAAA 
CCTGCCTACA CCTACAGCCG ACAGGAACAG GTTGCCCGTG TCCCAGTTTC TCGTGAGATC 
ATCCAGAATG GAAAGACACA AGTTACATAT CGTTCCAAGG ACCTGACTGC TAGGGAGGGA 
AAGGACTATA CATTCTCAGA GGGGGAGCTC ACCTTCCAGC CAGATGACAC TCAATCAGAG 
ATTCTAGTGC CTATACTCGG TCGTTCAGAA GTTGACACTC TGCTGGGAAA TAAACAAGTC 
AAACAGTTCC TTCTAGAACT CAGTAATCCT AAGTATGGGG CCAAGATTGG CAAATACCCA 
CAATGCACCA TCACTATTGA TGACACTGCA GATGTTATAC AAAAGACAAT GACAGTCCAG 
TCTGTTCAGT CCCCCCGTGG GAAGGTAGGT GCTCCTATCA ACCTGAATGC CCAGGCCCTC 
AGCGCCAGGA AAATCCGACT CAACTGGATG CCGCCTCCTG GAAAACCCAC AGGTTACAAG 
GTGCGTTACT GGATTCAAGG AGATTCCGAG TCAGATGCGA CTGTGGTGGA TACAAATGTG 
CCCTCTGCAG AGCTGGTCAA TCTGTACCCC TACTGTGACT ACGAGATGCG GGTATGCGCA 
TACAATGCTC AAGAGGAGGG TCCCTTCACT GACATAGTCA CCTGCCAGAC CTTGGAGGAA 
GCACCCAGTG AACCCGGCCG CCTCGCCTTT AACGTCATTT CAAGCACAGT GACGCAGCTG 
AGTTGGGCAG AGCCAGCTGA AACCAATGGT GAAATCACAG CGTATGAGGT CACCTACGGA 
ATGGTCAACG AGGACAACAA ACCCATTGGG CCCACGAAGA AAGTCCTGAT TAATGATTCA 
AAGAAGCGCA TGGTTCTAAT TGAAAACCTA CGAGAATCCC AGCCATACCG ATACACTGTG 
AGAGCCTGCA ACAAAGCTGG GTGGGGGCCA GAGAGAGATG CCACCATGAA CCTAGCTACC 
CAACCCACCC GCCCAATGTC TATTCCCATT ATCCCTGATG CTCCCATTAT TGATGCTGAA 
GCTGGGGAAG AATATGACAG TTACCTGATG TACAGCACCG ATGTCCTGCG CTCTCCAGTT 
GGCAGCAAGA GACCCAGTGT GTCCGACGAG TCAGGCCAGC GCTGGAAGTA TGTCCCTCTC 
CTGGGCTCGG AGATGGACCT GCGGCGGGTC ACCTGGAAGC TACCCGTAGA GGTCATACCA 
CGCCTGTCCG TCAGCAGCGG CCTGTCCACT GACAACGAGA GCTTGCTGGA CCAGCCTTTG 
ACTACCAGCT CACTGACGAG GAAAGGGACC ATGCGAGCAA CCCCAGAAGA GCAATTAATT 
AACGGACGCA CAGAATTCTC CTTCTCGGGC GGCAGTCTCA ACCGTTCCAT GGTATCAGGG 
TACCAGCAAC TCAGCCCGCA CGTGCAAAAT GAAAGCTACA GGTTTGGTAG CAGCAGTTCC 
CAGCATACAT CCACAACGGT CATATCAGGG CAAGGCTCAT CAGGGCAGAA CAGACTGGGG 
CTCCCCCCAT TGGTGTTAGG AGGAGGAAGG GGACGAACCC ACTCAGAGGA TGTGAGGGAT 
GCTCTGACAA GCTTGGATGT GGCCATACAG GATACACGTC TCTCCCCGGG AATCCCGGAT 
ACCCCTACCC GTCTGGTATT CTCAGCTCTG GGACCCACCT CCCTAAAGGT CAGTTGGCAG 
GAGCCGCAAT GCGAAAGAGA AGTTCTGGGA TACAAAGTGC ATTATCAACT ACTGCATGGA 
GGTGAATTAA GGACCATCGA CATTACAAAC CCACGGGAGA ACTCAGTAGT GGTGGAAGAC 
TTGCTTCCTA ACCACTCGTA CATGTTCCGT GTGAAGGCAC AAAGCGTTGA GGGCTGGGGG 
CCCGAAAGAG AAGGCGTTAT CACTATCGAA TCACAGGTGG ATCCTCAGAG TCCACTCAGC 
CCTGTTCCAG GGTCTCCATT CACCCTAAGC ACCCCCAGTG CTCCTGGACC ACTGGTTTTC 
ACAGCTCTGG CCCCTGACTC CCTGCAGTTA AGTTGGGTGA GACCCCGAAA GCCTAATGGA 
ACAATTCTGG GGTACATGAT CACTTGCGAG ATGTTGCATG GAGGAGGGGA ACCACGAACG 
ATCTATGTTG AAGGTGACAA ACCCGAGACT TCCCTGACGG TTCCTTTCCT AAATGAAAAT 
CTGCCCTATA AATTTAAGGT CCAGGCGAAA ACCACACAGG GTTTTGGACC AGAACGGGAA 
GGGATAATTA CTATTGAATC TCAAGATGGA GGAAACTTTT CTCAGTTTGG AACACAACAG 
ATCACACGCC GAGAGGTGTT CAATATGCCA GGAGAATATA CTCAAAGCAG CATCACTCAA 
TCCAACATAA CAGAACCCTT CTTTTCAGAT GGAATGAGTG TCACCACCCG CCGTATCGAG 
GGCTCCAACA CAGTCACACA ACAGGTCACC AAAGAATTTG TCACACGGAC CATGATGTCT 
GGCGGCTCCC TCAACAGGCA AGTGGAGCGC CAGTTTTACG ACGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940020.1
CDS	467..5974
Translation	MMNPRVLGGLLLLVLLLGLPASHSQSNKYNPCVASRATSCSACIRAYKDCSYCADEGFASVRCDLAENLRRYGCSERGIVYMRSEVLTQQSYDINTLVSKTQVAPQRMSMRLRAGEETSFDLNVFEPLDTPVDLYILMDFSHSMSDDLANLKNMGQELANVVSTLSKNYTIGFGKFVDKVTVPQTDMRPEKLKQPWLDSTPPFSFKNVIQLTSDVGKFRNELMKEKISGNLDPPEGGFDAILQTAVCTNQIGWRKGSTHLLVFSTESAFHYEADGMNVLDGILKRNDEQCHLDASGYYTHDIVQDYPSVPTLVRLLAKNNIIPIFAVTNYSYSYYDKLSKYFKVSEIGELKEDSSNIVELLQEAFTQIRSRMDIRDDNTPRAMDVSVTSETAKVTETGSFQVTRGEVGTFKVKVKALERLGNQHVCELPPQDQGSQILLKPSTFTDGLKIDASLICDSCPCELQKELSSPKCSFNGDFVCGRCECRERWRGVYCNCSSTDTKSTQSCISPGSTEICSGRGECLCGTCQCHSESPTQQYQGEFCQFNNFQCPRFLGYMCNDRGRCHMGACVCDAGWEGESCQCPTSNQTCLDRNGGLCSSRGKCVCGRCQCDNVSQYTDATCEFSYSLRQLGVCEDLRTCVQCQAWGTGEKKGTQCEACEIKIKMVDELKKVDERSENCTFRDETDDCTYEYSVDPHAVQGNYTVLVKNKKECPPGAFLWLIPLLIFLMLLLGLLLLLCWKYCACCKACLAMLPCCGPGRTVGFKEDHYMLRQSLMSSDYLNTPLVRSGSLKGGDTVRWKIDNNVHRQPPAPGTLNPKELVPYGVSLRLARLFTENLSKPQSRECTQLRQEVEDSLNEVYKVIPGTHKVKQTAFRLQSNAGKRQDHTIAETILMAPREAEPEIVKVTEKHVTQEAFQDMKVAPGYYTVTSDRDAHGLVEFQEGVELVDVRVPLFIREEDDDEKQLLVEALDVPSGIARLGRKLVNITIIKEQAKSIVTFVKPAYTYSRQEQVARVPVSREIIQNGKTQVTYRSKDLTAREGKDYTFSEGELTFQPDDTQSEILVPILGRSEVDTLLGNKQVKQFLLELSNPKYGAKIGKYPQCTITIDDTADVIQKTMTVQSVQSPRGKVGAPINLNAQALSARKIRLNWMPPPGKPTGYKVRYWIQGDSESDATVVDTNVPSAELVNLYPYCDYEMRVCAYNAQEEGPFTDIVTCQTLEEAPSEPGRLAFNVISSTVTQLSWAEPAETNGEITAYEVTYGMVNEDNKPIGPTKKVLINDSKKRMVLIENLRESQPYRYTVRACNKAGWGPERDATMNLATQPTRPMSIPIIPDAPIIDAEAGEEYDSYLMYSTDVLRSPVGSKRPSVSDESGQRWKYVPLLGSEMDLRRVTWKLPVEVIPRLSVSSGLSTDNESLLDQPLTTSSLTRKGTMRATPEEQLINGRTEFSFSGGSLNRSMVSGYQQLSPHVQNESYRFGSSSSQHTSTTVISGQGSSGQNRLGLPPLVLGGGRGRTHSEDVRDALTSLDVAIQDTRLSPGIPDTPTRLVFSALGPTSLKVSWQEPQCEREVLGYKVHYQLLHGGELRTIDITNPRENSVVVEDLLPNHSYMFRVKAQSVEGWGPEREGVITIESQVDPQSPLSPVPGSPFTLSTPSAPGPLVFTALAPDSLQLSWVRPRKPNGTILGYMITCEMLHGGGEPRTIYVEGDKPETSLTVPFLNENLPYKFKVQAKTTQGFGPEREGIITIESQDGGNFSQFGTQQITRREVFNMPGEYTQSSITQSNITEPFFSDGMSVTTRRIEGSNTVTQQVTKEFVTRTMMSGGSLNRQVERQFYDA

Target ORF information:

RefSeq Version	XM_002939974.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis integrin subunit beta 4 (itgb4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939974.5

ORF Insert Sequence:

ATGATGAACC CTCGTGTTTT AGGGGGTCTC CTCCTGCTTG TCCTGCTCTT GGGTCTCCCA 
GCTTCCCATT CTCAGAGCAA CAAATATAAC CCCTGCGTGG CCTCCAGAGC AACCAGTTGC 
TCGGCCTGTA TCCGGGCGTA CAAGGACTGT TCCTACTGTG CAGATGAGGG TTTCGCTAGT 
GTACGCTGTG ATCTAGCAGA GAATCTGAGA AGATATGGCT GCAGCGAGAG AGGGATTGTT 
TACATGAGAA GTGAAGTCTT GACCCAGCAG AGCTATGACA TTAACACTCT CGTCAGCAAA 
ACCCAAGTGG CCCCCCAAAG GATGTCCATG CGTCTTCGTG CTGGGGAAGA AACCTCATTT 
GACCTGAATG TGTTTGAGCC GCTGGACACC CCTGTGGATT TGTACATTTT GATGGACTTC 
TCTCATTCCA TGTCAGATGA TTTAGCCAAC CTAAAAAATA TGGGTCAGGA GTTAGCTAAT 
GTTGTAAGCA CTCTCTCCAA GAATTACACC ATTGGATTTG GAAAATTTGT GGACAAAGTG 
ACTGTACCAC AGACTGACAT GAGACCTGAG AAACTGAAGC AACCCTGGTT GGACAGCACT 
CCTCCTTTCT CCTTCAAGAA CGTTATTCAA CTGACAAGCG ACGTGGGGAA ATTTCGGAAT 
GAATTGATGA AAGAGAAAAT CTCTGGAAAC CTTGATCCTC CAGAGGGGGG GTTTGATGCC 
ATTCTTCAGA CTGCTGTGTG CACGAATCAA ATTGGCTGGA GAAAAGGAAG TACTCATCTG 
TTGGTCTTCT CTACTGAGTC AGCATTCCAT TATGAAGCTG ATGGGATGAA CGTACTTGAT 
GGGATACTGA AGCGAAATGA TGAGCAGTGC CACTTGGATG CCTCTGGTTA CTACACACAC 
GACATCGTCC AGGACTATCC ATCTGTGCCC ACACTTGTTC GACTGTTAGC AAAGAACAAC 
ATCATCCCTA TCTTTGCCGT CACCAACTAC TCCTACAGTT ACTATGATAA GCTGAGCAAG 
TATTTCAAAG TATCTGAGAT TGGAGAATTG AAAGAAGATT CCTCCAACAT TGTGGAGCTC 
TTGCAGGAGG CCTTTACGCA AATTCGTTCC AGGATGGATA TCCGAGATGA TAACACCCCA 
CGTGCAATGG ATGTCTCAGT CACTTCTGAG ACAGCTAAAG TTACAGAAAC AGGATCGTTT 
CAAGTCACCC GGGGAGAAGT GGGTACCTTC AAAGTGAAGG TCAAGGCTTT AGAACGGTTG 
GGCAACCAGC ATGTGTGTGA ACTACCACCA CAAGACCAAG GGAGCCAAAT TCTCCTCAAA 
CCTTCCACCT TTACTGATGG CCTTAAGATT GATGCTTCTC TGATCTGTGA CAGCTGTCCT 
TGTGAATTGC AAAAGGAGTT GAGTTCCCCC AAGTGCAGCT TTAATGGCGA CTTTGTGTGC 
GGACGCTGTG AGTGCAGGGA GCGCTGGAGG GGAGTATATT GTAACTGCTC CAGCACAGAC 
ACCAAAAGCA CCCAATCGTG TATATCACCA GGCAGTACAG AGATTTGCTC CGGCAGGGGT 
GAATGCCTAT GCGGCACCTG TCAGTGTCAC TCGGAGTCCC CCACTCAACA GTACCAGGGG 
GAGTTCTGCC AGTTCAATAA CTTCCAGTGC CCACGTTTTC TTGGATACAT GTGCAATGAC 
AGAGGGCGCT GTCACATGGG AGCCTGTGTG TGTGATGCTG GCTGGGAAGG AGAGAGTTGT 
CAGTGTCCTA CCAGCAACCA AACCTGCCTT GATAGGAATG GGGGTCTGTG CAGTAGCCGT 
GGGAAGTGCG TGTGTGGGCG CTGCCAGTGT GATAACGTGT CACAGTATAC AGACGCCACG 
TGTGAATTCT CCTATTCACT GCGCCAACTG GGTGTGTGCG AAGACCTGCG GACGTGTGTG 
CAGTGCCAGG CCTGGGGAAC TGGAGAGAAA AAGGGCACAC AATGTGAAGC TTGTGAAATT 
AAGATTAAAA TGGTGGATGA GCTGAAGAAA GTGGATGAAA GAAGTGAAAA CTGTACTTTC 
CGGGATGAAA CAGATGACTG TACATATGAG TACTCAGTAG ACCCTCACGC AGTGCAAGGA 
AACTACACTG TGCTGGTCAA GAACAAAAAA GAATGTCCTC CTGGAGCCTT CCTCTGGCTT 
ATCCCTCTAC TCATCTTTCT GATGCTTCTC CTTGGGCTCT TGTTGCTCCT GTGCTGGAAA 
TACTGTGCCT GTTGCAAGGC TTGCTTGGCA ATGCTTCCCT GCTGTGGACC AGGACGGACA 
GTGGGATTTA AAGAAGACCA CTACATGTTA AGGCAAAGCC TGATGTCATC TGATTACCTG 
AATACCCCAC TGGTGCGTTC TGGGAGCCTC AAGGGCGGGG ACACTGTAAG ATGGAAGATC 
GACAACAACG TGCACCGCCA ACCACCCGCC CCTGGAACAC TGAACCCCAA GGAGCTTGTC 
CCATATGGCG TCTCACTGCG TCTGGCCCGG CTGTTCACTG AGAACCTGTC CAAGCCTCAA 
TCCCGTGAGT GTACCCAGCT GCGTCAGGAG GTGGAAGATT CGTTAAATGA GGTGTACAAG 
GTGATACCTG GAACCCACAA GGTGAAACAG ACAGCTTTCA GGCTTCAGTC CAATGCAGGA 
AAAAGACAGG ATCACACTAT TGCAGAAACC ATCCTGATGG CTCCCCGGGA GGCTGAACCT 
GAGATTGTAA AGGTTACAGA GAAACATGTA ACACAAGAGG CTTTCCAGGA TATGAAGGTT 
GCCCCAGGTT ACTATACAGT GACGTCAGAC AGAGATGCCC ATGGACTTGT AGAGTTCCAG 
GAGGGAGTTG AATTGGTGGA TGTCCGGGTT CCTCTCTTCA TCAGGGAAGA GGATGATGAT 
GAGAAGCAGT TGTTAGTGGA AGCTCTGGAC GTTCCATCAG GAATTGCCCG TTTGGGGCGG 
AAACTGGTCA ATATTACTAT TATCAAAGAG CAAGCCAAGA GCATAGTGAC GTTTGTGAAA 
CCTGCCTACA CCTACAGCCG ACAGGAACAG GTTGCCCGTG TCCCAGTTTC TCGTGAGATC 
ATCCAGAATG GAAAGACACA AGTTACATAT CGTTCCAAGG ACCTGACTGC TAGGGAGGGA 
AAGGACTATA CATTCTCAGA GGGGGAGCTC ACCTTCCAGC CAGATGACAC TCAATCAGAG 
ATTCTAGTGC CTATACTCGG TCGTTCAGAA GTTGACACTC TGCTGGGAAA TAAACAAGTC 
AAACAGTTCC TTCTAGAACT CAGTAATCCT AAGTATGGGG CCAAGATTGG CAAATACCCA 
CAATGCACCA TCACTATTGA TGACACTGCA GATGTTATAC AAAAGACAAT GACAGTCCAG 
TCTGTTCAGT CCCCCCGTGG GAAGGTAGGT GCTCCTATCA ACCTGAATGC CCAGGCCCTC 
AGCGCCAGGA AAATCCGACT CAACTGGATG CCGCCTCCTG GAAAACCCAC AGGTTACAAG 
GTGCGTTACT GGATTCAAGG AGATTCCGAG TCAGATGCGA CTGTGGTGGA TACAAATGTG 
CCCTCTGCAG AGCTGGTCAA TCTGTACCCC TACTGTGACT ACGAGATGCG GGTATGCGCA 
TACAATGCTC AAGAGGAGGG TCCCTTCACT GACATAGTCA CCTGCCAGAC CTTGGAGGAA 
GCACCCAGTG AACCCGGCCG CCTCGCCTTT AACGTCATTT CAAGCACAGT GACGCAGCTG 
AGTTGGGCAG AGCCAGCTGA AACCAATGGT GAAATCACAG CGTATGAGGT CACCTACGGA 
ATGGTCAACG AGGACAACAA ACCCATTGGG CCCACGAAGA AAGTCCTGAT TAATGATTCA 
AAGAAGCGCA TGGTTCTAAT TGAAAACCTA CGAGAATCCC AGCCATACCG ATACACTGTG 
AGAGCCTGCA ACAAAGCTGG GTGGGGGCCA GAGAGAGATG CCACCATGAA CCTAGCTACC 
CAACCCACCC GCCCAATGTC TATTCCCATT ATCCCTGATG CTCCCATTAT TGATGCTGAA 
GCTGGGGAAG AATATGACAG TTACCTGATG TACAGCACCG ATGTCCTGCG CTCTCCAGTT 
GGCAGCAAGA GACCCAGTGT GTCCGACGAG TCAGGCCAGC GCTGGAAGTA TGTCCCTCTC 
CTGGGCTCGG AGATGGACCT GCGGCGGGTC ACCTGGAAGC TACCCGTAGA GGTCATACCA 
CGCCTGTCCG TCAGCAGCGG CCTGTCCACT GACAACGAGA GCTTGCTGGA CCAGCCTTTG 
ACTACCAGCT CACTGACGAG GAAAGGGACC ATGCGAGCAA CCCCAGAAGA GCAATTAATT 
AACGGACGCA CAGAATTCTC CTTCTCGGGC GGCAGTCTCA ACCGTTCCAT GGTATCAGGG 
TACCAGCAAC TCAGCCCGCA CGTGCAAAAT GAAAGCTACA GGTTTGGTAG CAGCAGTTCC 
CAGCATACAT CCACAACGGT CATATCAGGG CAAGGCTCAT CAGGGCAGAA CAGACTGGGG 
CTCCCCCCAT TGGTGTTAGG AGGAGGAAGG GGACGAACCC ACTCAGAGGA TGTGAGGGAT 
GCTCTGACAA GCTTGGATGT GGCCATACAG GATACACGTC TCTCCCCGGG AATCCCGGAT 
ACCCCTACCC GTCTGGTATT CTCAGCTCTG GGACCCACCT CCCTAAAGGT CAGTTGGCAG 
GAGCCGCAAT GCGAAAGAGA AGTTCTGGGA TACAAAGTGC ATTATCAACT ACTGCATGGA 
GGTGAATTAA GGACCATCGA CATTACAAAC CCACGGGAGA ACTCAGTAGT GGTGGAAGAC 
TTGCTTCCTA ACCACTCGTA CATGTTCCGT GTGAAGGCAC AAAGCGTTGA GGGCTGGGGG 
CCCGAAAGAG AAGGCGTTAT CACTATCGAA TCACAGGTGG ATCCTCAGAG TCCACTCAGC 
CCTGTTCCAG GGTCTCCATT CACCCTAAGC ACCCCCAGTG CTCCTGGACC ACTGGTTTTC 
ACAGCTCTGG CCCCTGACTC CCTGCAGTTA AGTTGGGTGA GACCCCGAAA GCCTAATGGA 
ACAATTCTGG GGTACATGAT CACTTGCGAG ATGTTGCATG GAGGAGGGGA ACCACGAACG 
ATCTATGTTG AAGGTGACAA ACCCGAGACT TCCCTGACGG TTCCTTTCCT AAATGAAAAT 
CTGCCCTATA AATTTAAGGT CCAGGCGAAA ACCACACAGG GTTTTGGACC AGAACGGGAA 
GGGATAATTA CTATTGAATC TCAAGATGGA GGAAACTTTT CTCAGTTTGG AACACAACAG 
ATCACACGCC GAGAGGTGTT CAATATGCCA GGAGAATATA CTCAAAGCAG CATCACTCAA 
TCCAACATAA CAGAACCCTT CTTTTCAGAT GGAATGAGTG TCACCACCCG CCGTATCGAG 
GGCTCCAACA CAGTCACACA ACAGGTCACC AAAGAATTTG TCACACGGAC CATGATGTCT 
GGCGGCTCCC TCAACAGGCA AGTGGAGCGC CAGTTTTACG ACGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itgb4 ( XM_004918457.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004918514.1 Xenopus tropicalis integrin subunit beta 4 (itgb4), transcript variant X3, mRNA.

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CloneID	OXa50384
Clone ID Related Accession (Same CDS sequence)	XM_004918457.4
Accession Version	XM_004918457.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	integrin beta-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004918457.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAACC CTCGTGTTTT AGGGGGTCTC CTCCTGCTTG TCCTGCTCTT GGGTCTCCCA 
GCTTCCCATT CTCAGAGCAA CAAATATAAC CCCTGCGTGG CCTCCAGAGC AACCAGTTGC 
TCGGCCTGTA TCCGGGCGTA CAAGGACTGT TCCTACTGTG CAGATGAGGG TTTCGCTAGT 
GTACGCTGTG ATCTAGCAGA GAATCTGAGA AGATATGGCT GCAGCGAGAG AGGGATTGTT 
TACATGAGAA GTGAAGTCTT GACCCAGCAG AGCTATGACA TTAACACTCT CGTCAGCAAA 
ACCCAAGTGG CCCCCCAAAG GATGTCCATG CGTCTTCGTG CTGGGGAAGA AACCTCATTT 
GACCTGAATG TGTTTGAGCC GCTGGACACC CCTGTGGATT TGTACATTTT GATGGACTTC 
TCTCATTCCA TGTCAGATGA TTTAGCCAAC CTAAAAAATA TGGGTCAGGA GTTAGCTAAT 
GTTGTAAGCA CTCTCTCCAA GAATTACACC ATTGGATTTG GAAAATTTGT GGACAAAGTG 
ACTGTACCAC AGACTGACAT GAGACCTGAG AAACTGAAGC AACCCTGGTT GGACAGCACT 
CCTCCTTTCT CCTTCAAGAA CGTTATTCAA CTGACAAGCG ACGTGGGGAA ATTTCGGAAT 
GAATTGATGA AAGAGAAAAT CTCTGGAAAC CTTGATCCTC CAGAGGGGGG GTTTGATGCC 
ATTCTTCAGA CTGCTGTGTG CACGAATCAA ATTGGCTGGA GAAAAGGAAG TACTCATCTG 
TTGGTCTTCT CTACTGAGTC AGCATTCCAT TATGAAGCTG ATGGGATGAA CGTACTTGAT 
GGGATACTGA AGCGAAATGA TGAGCAGTGC CACTTGGATG CCTCTGGTTA CTACACACAC 
GACATCGTCC AGGACTATCC ATCTGTGCCC ACACTTGTTC GACTGTTAGC AAAGAACAAC 
ATCATCCCTA TCTTTGCCGT CACCAACTAC TCCTACAGTT ACTATGATAA GCTGAGCAAG 
TATTTCAAAG TATCTGAGAT TGGAGAATTG AAAGAAGATT CCTCCAACAT TGTGGAGCTC 
TTGCAGGAGG CCTTTACGCA AATTCGTTCC AGGATGGATA TCCGAGATGA TAACACCCCA 
CGTGCAATGG ATGTCTCAGT CACTTCTGAG ACAGCTAAAG TTACAGAAAC AGGATCGTTT 
CAAGTCACCC GGGGAGAAGT GGGTACCTTC AAAGTGAAGG TCAAGGCTTT AGAACGGTTG 
GGCAACCAGC ATGTGTGTGA ACTACCACCA CAAGACCAAG GGAGCCAAAT TCTCCTCAAA 
CCTTCCACCT TTACTGATGG CCTTAAGATT GATGCTTCTC TGATCTGTGA CAGCTGTCCT 
TGTGAATTGC AAAAGGAGTT GAGTTCCCCC AAGTGCAGCT TTAATGGCGA CTTTGTGTGC 
GGACGCTGTG AGTGCAGGGA GCGCTGGAGG GGAGTATATT GTAACTGCTC CAGCACAGAC 
ACCAAAAGCA CCCAATCGTG TATATCACCA GGCAGTACAG AGATTTGCTC CGGCAGGGGT 
GAATGCCTAT GCGGCACCTG TCAGTGTCAC TCGGAGTCCC CCACTCAACA GTACCAGGGG 
GAGTTCTGCC AGTTCAATAA CTTCCAGTGC CCACGTTTTC TTGGATACAT GTGCAATGAC 
AGAGGGCGCT GTCACATGGG AGCCTGTGTG TGTGATGCTG GCTGGGAAGG AGAGAGTTGT 
CAGTGTCCTA CCAGCAACCA AACCTGCCTT GATAGGAATG GGGGTCTGTG CAGTAGCCGT 
GGGAAGTGCG TGTGTGGGCG CTGCCAGTGT GATAACGTGT CACAGTATAC AGACGCCACG 
TGTGAATTCT CCTATTCACT GCGCCAACTG GGTGTGTGCG AAGACCTGCG GACGTGTGTG 
CAGTGCCAGG CCTGGGGAAC TGGAGAGAAA AAGGGCACAC AATGTGAAGC TTGTGAAATT 
AAGATTAAAA TGGTGGATGA GCTGAAGAAA GTGGATGAAA GAAGTGAAAA CTGTACTTTC 
CGGGATGAAA CAGATGACTG TACATATGAG TACTCAGTAG ACCCTCACGC AGTGCAAGGA 
AACTACACTG TGCTGGTCAA GAACAAAAAA GAATGTCCTC CTGGAGCCTT CCTCTGGCTT 
ATCCCTCTAC TCATCTTTCT GATGCTTCTC CTTGGGCTCT TGTTGCTCCT GTGCTGGAAA 
TACTGTGCCT GTTGCAAGGC TTGCTTGGCA ATGCTTCCCT GCTGTGGACC AGGACGGACA 
GTGGGATTTA AAGAAGACCA CTACATGTTA AGGCAAAGCC TGATGTCATC TGATTACCTG 
AATACCCCAC TGGTGCGTTC TGGGAGCCTC AAGGGCGGGG ACACTGTAAG ATGGAAGATC 
GACAACAACG TGCACCGCCA ACCACCCGCC CCTGGAACAC TGAACCCCAA GGAGCTTGTC 
CCATATGGCG TCTCACTGCG TCTGGCCCGG CTGTTCACTG AGAACCTGTC CAAGCCTCAA 
TCCCGTGAGT GTACCCAGCT GCGTCAGGAG GTGGAAGATT CGTTAAATGA GGTGTACAAG 
GTGATACCTG GAACCCACAA GGTGAAACAG ACAGCTTTCA GGCTTCAGTC CAATGCAGGA 
AAAAGACAGG ATCACACTAT TGCAGAAACC ATCCTGATGG CTCCCCGGGA GGCTGAACCT 
GAGATTGTAA AGGTTACAGA GAAACATGTA ACACAAGAGG CTTTCCAGGA TATGAAGGTT 
GCCCCAGGTT ACTATACAGT GACGTCAGAC AGAGATGCCC ATGGACTTGT AGAGTTCCAG 
GAGGGAGTTG AATTGGTGGA TGTCCGGGTT CCTCTCTTCA TCAGGGAAGA GGATGATGAT 
GAGAAGCAGT TGTTAGTGGA AGCTCTGGAC GTTCCATCAG GAATTGCCCG TTTGGGGCGG 
AAACTGGTCA ATATTACTAT TATCAAAGAG CAAGCCAAGA GCATAGTGAC GTTTGTGAAA 
CCTGCCTACA CCTACAGCCG ACAGGAACAG GTTGCCCGTG TCCCAGTTTC TCGTGAGATC 
ATCCAGAATG GAAAGACACA AGTTACATAT CGTTCCAAGG ACCTGACTGC TAGGGAGGGA 
AAGGACTATA CATTCTCAGA GGGGGAGCTC ACCTTCCAGC CAGATGACAC TCAATCAGAG 
ATTCTAGTGC CTATACTCGG TCGTTCAGAA GTTGACACTC TGCTGGGAAA TAAACAAGTC 
AAACAGTTCC TTCTAGAACT CAGTAATCCT AAGTATGGGG CCAAGATTGG CAAATACCCA 
CAATGCACCA TCACTATTGA TGACACTGCA GATGTTATAC AAAAGACAAT GACAGTCCAG 
TCTGTTCAGT CCCCCCGTGG GAAGGTAGGT GCTCCTATCA ACCTGAATGC CCAGGCCCTC 
AGCGCCAGGA AAATCCGACT CAACTGGATG CCGCCTCCTG GAAAACCCAC AGGTTACAAG 
GTGCGTTACT GGATTCAAGG AGATTCCGAG TCAGATGCGA CTGTGGTGGA TACAAATGTG 
CCCTCTGCAG AGCTGGTCAA TCTGTACCCC TACTGTGACT ACGAGATGCG GGTATGCGCA 
TACAATGCTC AAGAGGAGGG TCCCTTCACT GACATAGTCA CCTGCCAGAC CTTGGAGGAA 
GCACCCAGTG AACCCGGCCG CCTCGCCTTT AACGTCATTT CAAGCACAGT GACGCAGCTG 
AGTTGGGCAG AGCCAGCTGA AACCAATGGT GAAATCACAG CGTATGAGGT CACCTACGGA 
ATGGTCAACG AGGACAACAA ACCCATTGGG CCCACGAAGA AAGTCCTGAT TAATGATTCA 
AAGAAGCGCA TGGTTCTAAT TGAAAACCTA CGAGAATCCC AGCCATACCG ATACACTGTG 
AGAGCCTGCA ACAAAGCTGG GTGGGGGCCA GAGAGAGATG CCACCATGAA CCTAGCTACC 
CAACCCACCC GCCCAATGTC TATTCCCATT ATCCCTGATG CTCCCATTAT TGATGCTGAA 
GCTGGGGAAG AATATGACAG TTACCTGATG TACAGCACCG ATGTCCTGCG CTCTCCAGTT 
GGCAGCAAGA GACCCAGTGT GTCCGACGAG TCAGAGCAAT TAATTAACGG ACGCACAGAA 
TTCTCCTTCT CGGGCGGCAG TCTCAACCGT TCCATGGTAT CAGGGTACCA GCAACTCAGC 
CCGCACGTGC AAAATGAAAG CTACAGGTTT GGTAGCAGCA GTTCCCAGCA TACATCCACA 
ACGGTCATAT CAGGGCAAGG CTCATCAGGG CAGAACAGAC TGGGGCTCCC CCCATTGGTG 
TTAGGAGGAG GAAGGGGACG AACCCACTCA GAGGATGTGA GGGATGCTCT GACAAGCTTG 
GATGTGGCCA TACAGGATAC ACGTCTCTCC CCGGGAATCC CGGATACCCC TACCCGTCTG 
GTATTCTCAG CTCTGGGACC CACCTCCCTA AAGGTCAGTT GGCAGGAGCC GCAATGCGAA 
AGAGAAGTTC TGGGATACAA AGTGCATTAT CAACTACTGC ATGGAGGTGA ATTAAGGACC 
ATCGACATTA CAAACCCACG GGAGAACTCA GTAGTGGTGG AAGACTTGCT TCCTAACCAC 
TCGTACATGT TCCGTGTGAA GGCACAAAGC GTTGAGGGCT GGGGGCCCGA AAGAGAAGGC 
GTTATCACTA TCGAATCACA GGTGGATCCT CAGAGTCCAC TCAGCCCTGT TCCAGGGTCT 
CCATTCACCC TAAGCACCCC CAGTGCTCCT GGACCACTGG TTTTCACAGC TCTGGCCCCT 
GACTCCCTGC AGTTAAGTTG GGTGAGACCC CGAAAGCCTA ATGGAACAAT TCTGGGGTAC 
ATGATCACTT GCGAGATGTT GCATGGAGGA GGGGAACCAC GAACGATCTA TGTTGAAGGT 
GACAAACCCG AGACTTCCCT GACGGTTCCT TTCCTAAATG AAAATCTGCC CTATAAATTT 
AAGGTCCAGG CGAAAACCAC ACAGGGTTTT GGACCAGAAC GGGAAGGGAT AATTACTATT 
GAATCTCAAG ATGGAGGAAA CTTTTCTCAG TTTGGAACAC AACAGATCAC ACGCCGAGAG 
GTGTTCAATA TGCCAGGAGA ATATACTCAA AGCAGCATCA CTCAATCCAA CATAACAGAA 
CCCTTCTTTT CAGATGGAAT GAGTGTCACC ACCCGCCGTA TCGAGGGCTC CAACACAGTC 
ACACAACAGG TCACCAAAGA ATTTGTCACA CGGACCATGA TGTCTGGCGG CTCCCTCAAC 
AGGCAAGTGG AGCGCCAGTT TTACGACGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004918514.1
CDS	462..5774
Translation	MMNPRVLGGLLLLVLLLGLPASHSQSNKYNPCVASRATSCSACIRAYKDCSYCADEGFASVRCDLAENLRRYGCSERGIVYMRSEVLTQQSYDINTLVSKTQVAPQRMSMRLRAGEETSFDLNVFEPLDTPVDLYILMDFSHSMSDDLANLKNMGQELANVVSTLSKNYTIGFGKFVDKVTVPQTDMRPEKLKQPWLDSTPPFSFKNVIQLTSDVGKFRNELMKEKISGNLDPPEGGFDAILQTAVCTNQIGWRKGSTHLLVFSTESAFHYEADGMNVLDGILKRNDEQCHLDASGYYTHDIVQDYPSVPTLVRLLAKNNIIPIFAVTNYSYSYYDKLSKYFKVSEIGELKEDSSNIVELLQEAFTQIRSRMDIRDDNTPRAMDVSVTSETAKVTETGSFQVTRGEVGTFKVKVKALERLGNQHVCELPPQDQGSQILLKPSTFTDGLKIDASLICDSCPCELQKELSSPKCSFNGDFVCGRCECRERWRGVYCNCSSTDTKSTQSCISPGSTEICSGRGECLCGTCQCHSESPTQQYQGEFCQFNNFQCPRFLGYMCNDRGRCHMGACVCDAGWEGESCQCPTSNQTCLDRNGGLCSSRGKCVCGRCQCDNVSQYTDATCEFSYSLRQLGVCEDLRTCVQCQAWGTGEKKGTQCEACEIKIKMVDELKKVDERSENCTFRDETDDCTYEYSVDPHAVQGNYTVLVKNKKECPPGAFLWLIPLLIFLMLLLGLLLLLCWKYCACCKACLAMLPCCGPGRTVGFKEDHYMLRQSLMSSDYLNTPLVRSGSLKGGDTVRWKIDNNVHRQPPAPGTLNPKELVPYGVSLRLARLFTENLSKPQSRECTQLRQEVEDSLNEVYKVIPGTHKVKQTAFRLQSNAGKRQDHTIAETILMAPREAEPEIVKVTEKHVTQEAFQDMKVAPGYYTVTSDRDAHGLVEFQEGVELVDVRVPLFIREEDDDEKQLLVEALDVPSGIARLGRKLVNITIIKEQAKSIVTFVKPAYTYSRQEQVARVPVSREIIQNGKTQVTYRSKDLTAREGKDYTFSEGELTFQPDDTQSEILVPILGRSEVDTLLGNKQVKQFLLELSNPKYGAKIGKYPQCTITIDDTADVIQKTMTVQSVQSPRGKVGAPINLNAQALSARKIRLNWMPPPGKPTGYKVRYWIQGDSESDATVVDTNVPSAELVNLYPYCDYEMRVCAYNAQEEGPFTDIVTCQTLEEAPSEPGRLAFNVISSTVTQLSWAEPAETNGEITAYEVTYGMVNEDNKPIGPTKKVLINDSKKRMVLIENLRESQPYRYTVRACNKAGWGPERDATMNLATQPTRPMSIPIIPDAPIIDAEAGEEYDSYLMYSTDVLRSPVGSKRPSVSDESEQLINGRTEFSFSGGSLNRSMVSGYQQLSPHVQNESYRFGSSSSQHTSTTVISGQGSSGQNRLGLPPLVLGGGRGRTHSEDVRDALTSLDVAIQDTRLSPGIPDTPTRLVFSALGPTSLKVSWQEPQCEREVLGYKVHYQLLHGGELRTIDITNPRENSVVVEDLLPNHSYMFRVKAQSVEGWGPEREGVITIESQVDPQSPLSPVPGSPFTLSTPSAPGPLVFTALAPDSLQLSWVRPRKPNGTILGYMITCEMLHGGGEPRTIYVEGDKPETSLTVPFLNENLPYKFKVQAKTTQGFGPEREGIITIESQDGGNFSQFGTQQITRREVFNMPGEYTQSSITQSNITEPFFSDGMSVTTRRIEGSNTVTQQVTKEFVTRTMMSGGSLNRQVERQFYDA

Target ORF information:

RefSeq Version	XM_004918457.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis integrin subunit beta 4 (itgb4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004918457.4

ORF Insert Sequence:

ATGATGAACC CTCGTGTTTT AGGGGGTCTC CTCCTGCTTG TCCTGCTCTT GGGTCTCCCA 
GCTTCCCATT CTCAGAGCAA CAAATATAAC CCCTGCGTGG CCTCCAGAGC AACCAGTTGC 
TCGGCCTGTA TCCGGGCGTA CAAGGACTGT TCCTACTGTG CAGATGAGGG TTTCGCTAGT 
GTACGCTGTG ATCTAGCAGA GAATCTGAGA AGATATGGCT GCAGCGAGAG AGGGATTGTT 
TACATGAGAA GTGAAGTCTT GACCCAGCAG AGCTATGACA TTAACACTCT CGTCAGCAAA 
ACCCAAGTGG CCCCCCAAAG GATGTCCATG CGTCTTCGTG CTGGGGAAGA AACCTCATTT 
GACCTGAATG TGTTTGAGCC GCTGGACACC CCTGTGGATT TGTACATTTT GATGGACTTC 
TCTCATTCCA TGTCAGATGA TTTAGCCAAC CTAAAAAATA TGGGTCAGGA GTTAGCTAAT 
GTTGTAAGCA CTCTCTCCAA GAATTACACC ATTGGATTTG GAAAATTTGT GGACAAAGTG 
ACTGTACCAC AGACTGACAT GAGACCTGAG AAACTGAAGC AACCCTGGTT GGACAGCACT 
CCTCCTTTCT CCTTCAAGAA CGTTATTCAA CTGACAAGCG ACGTGGGGAA ATTTCGGAAT 
GAATTGATGA AAGAGAAAAT CTCTGGAAAC CTTGATCCTC CAGAGGGGGG GTTTGATGCC 
ATTCTTCAGA CTGCTGTGTG CACGAATCAA ATTGGCTGGA GAAAAGGAAG TACTCATCTG 
TTGGTCTTCT CTACTGAGTC AGCATTCCAT TATGAAGCTG ATGGGATGAA CGTACTTGAT 
GGGATACTGA AGCGAAATGA TGAGCAGTGC CACTTGGATG CCTCTGGTTA CTACACACAC 
GACATCGTCC AGGACTATCC ATCTGTGCCC ACACTTGTTC GACTGTTAGC AAAGAACAAC 
ATCATCCCTA TCTTTGCCGT CACCAACTAC TCCTACAGTT ACTATGATAA GCTGAGCAAG 
TATTTCAAAG TATCTGAGAT TGGAGAATTG AAAGAAGATT CCTCCAACAT TGTGGAGCTC 
TTGCAGGAGG CCTTTACGCA AATTCGTTCC AGGATGGATA TCCGAGATGA TAACACCCCA 
CGTGCAATGG ATGTCTCAGT CACTTCTGAG ACAGCTAAAG TTACAGAAAC AGGATCGTTT 
CAAGTCACCC GGGGAGAAGT GGGTACCTTC AAAGTGAAGG TCAAGGCTTT AGAACGGTTG 
GGCAACCAGC ATGTGTGTGA ACTACCACCA CAAGACCAAG GGAGCCAAAT TCTCCTCAAA 
CCTTCCACCT TTACTGATGG CCTTAAGATT GATGCTTCTC TGATCTGTGA CAGCTGTCCT 
TGTGAATTGC AAAAGGAGTT GAGTTCCCCC AAGTGCAGCT TTAATGGCGA CTTTGTGTGC 
GGACGCTGTG AGTGCAGGGA GCGCTGGAGG GGAGTATATT GTAACTGCTC CAGCACAGAC 
ACCAAAAGCA CCCAATCGTG TATATCACCA GGCAGTACAG AGATTTGCTC CGGCAGGGGT 
GAATGCCTAT GCGGCACCTG TCAGTGTCAC TCGGAGTCCC CCACTCAACA GTACCAGGGG 
GAGTTCTGCC AGTTCAATAA CTTCCAGTGC CCACGTTTTC TTGGATACAT GTGCAATGAC 
AGAGGGCGCT GTCACATGGG AGCCTGTGTG TGTGATGCTG GCTGGGAAGG AGAGAGTTGT 
CAGTGTCCTA CCAGCAACCA AACCTGCCTT GATAGGAATG GGGGTCTGTG CAGTAGCCGT 
GGGAAGTGCG TGTGTGGGCG CTGCCAGTGT GATAACGTGT CACAGTATAC AGACGCCACG 
TGTGAATTCT CCTATTCACT GCGCCAACTG GGTGTGTGCG AAGACCTGCG GACGTGTGTG 
CAGTGCCAGG CCTGGGGAAC TGGAGAGAAA AAGGGCACAC AATGTGAAGC TTGTGAAATT 
AAGATTAAAA TGGTGGATGA GCTGAAGAAA GTGGATGAAA GAAGTGAAAA CTGTACTTTC 
CGGGATGAAA CAGATGACTG TACATATGAG TACTCAGTAG ACCCTCACGC AGTGCAAGGA 
AACTACACTG TGCTGGTCAA GAACAAAAAA GAATGTCCTC CTGGAGCCTT CCTCTGGCTT 
ATCCCTCTAC TCATCTTTCT GATGCTTCTC CTTGGGCTCT TGTTGCTCCT GTGCTGGAAA 
TACTGTGCCT GTTGCAAGGC TTGCTTGGCA ATGCTTCCCT GCTGTGGACC AGGACGGACA 
GTGGGATTTA AAGAAGACCA CTACATGTTA AGGCAAAGCC TGATGTCATC TGATTACCTG 
AATACCCCAC TGGTGCGTTC TGGGAGCCTC AAGGGCGGGG ACACTGTAAG ATGGAAGATC 
GACAACAACG TGCACCGCCA ACCACCCGCC CCTGGAACAC TGAACCCCAA GGAGCTTGTC 
CCATATGGCG TCTCACTGCG TCTGGCCCGG CTGTTCACTG AGAACCTGTC CAAGCCTCAA 
TCCCGTGAGT GTACCCAGCT GCGTCAGGAG GTGGAAGATT CGTTAAATGA GGTGTACAAG 
GTGATACCTG GAACCCACAA GGTGAAACAG ACAGCTTTCA GGCTTCAGTC CAATGCAGGA 
AAAAGACAGG ATCACACTAT TGCAGAAACC ATCCTGATGG CTCCCCGGGA GGCTGAACCT 
GAGATTGTAA AGGTTACAGA GAAACATGTA ACACAAGAGG CTTTCCAGGA TATGAAGGTT 
GCCCCAGGTT ACTATACAGT GACGTCAGAC AGAGATGCCC ATGGACTTGT AGAGTTCCAG 
GAGGGAGTTG AATTGGTGGA TGTCCGGGTT CCTCTCTTCA TCAGGGAAGA GGATGATGAT 
GAGAAGCAGT TGTTAGTGGA AGCTCTGGAC GTTCCATCAG GAATTGCCCG TTTGGGGCGG 
AAACTGGTCA ATATTACTAT TATCAAAGAG CAAGCCAAGA GCATAGTGAC GTTTGTGAAA 
CCTGCCTACA CCTACAGCCG ACAGGAACAG GTTGCCCGTG TCCCAGTTTC TCGTGAGATC 
ATCCAGAATG GAAAGACACA AGTTACATAT CGTTCCAAGG ACCTGACTGC TAGGGAGGGA 
AAGGACTATA CATTCTCAGA GGGGGAGCTC ACCTTCCAGC CAGATGACAC TCAATCAGAG 
ATTCTAGTGC CTATACTCGG TCGTTCAGAA GTTGACACTC TGCTGGGAAA TAAACAAGTC 
AAACAGTTCC TTCTAGAACT CAGTAATCCT AAGTATGGGG CCAAGATTGG CAAATACCCA 
CAATGCACCA TCACTATTGA TGACACTGCA GATGTTATAC AAAAGACAAT GACAGTCCAG 
TCTGTTCAGT CCCCCCGTGG GAAGGTAGGT GCTCCTATCA ACCTGAATGC CCAGGCCCTC 
AGCGCCAGGA AAATCCGACT CAACTGGATG CCGCCTCCTG GAAAACCCAC AGGTTACAAG 
GTGCGTTACT GGATTCAAGG AGATTCCGAG TCAGATGCGA CTGTGGTGGA TACAAATGTG 
CCCTCTGCAG AGCTGGTCAA TCTGTACCCC TACTGTGACT ACGAGATGCG GGTATGCGCA 
TACAATGCTC AAGAGGAGGG TCCCTTCACT GACATAGTCA CCTGCCAGAC CTTGGAGGAA 
GCACCCAGTG AACCCGGCCG CCTCGCCTTT AACGTCATTT CAAGCACAGT GACGCAGCTG 
AGTTGGGCAG AGCCAGCTGA AACCAATGGT GAAATCACAG CGTATGAGGT CACCTACGGA 
ATGGTCAACG AGGACAACAA ACCCATTGGG CCCACGAAGA AAGTCCTGAT TAATGATTCA 
AAGAAGCGCA TGGTTCTAAT TGAAAACCTA CGAGAATCCC AGCCATACCG ATACACTGTG 
AGAGCCTGCA ACAAAGCTGG GTGGGGGCCA GAGAGAGATG CCACCATGAA CCTAGCTACC 
CAACCCACCC GCCCAATGTC TATTCCCATT ATCCCTGATG CTCCCATTAT TGATGCTGAA 
GCTGGGGAAG AATATGACAG TTACCTGATG TACAGCACCG ATGTCCTGCG CTCTCCAGTT 
GGCAGCAAGA GACCCAGTGT GTCCGACGAG TCAGAGCAAT TAATTAACGG ACGCACAGAA 
TTCTCCTTCT CGGGCGGCAG TCTCAACCGT TCCATGGTAT CAGGGTACCA GCAACTCAGC 
CCGCACGTGC AAAATGAAAG CTACAGGTTT GGTAGCAGCA GTTCCCAGCA TACATCCACA 
ACGGTCATAT CAGGGCAAGG CTCATCAGGG CAGAACAGAC TGGGGCTCCC CCCATTGGTG 
TTAGGAGGAG GAAGGGGACG AACCCACTCA GAGGATGTGA GGGATGCTCT GACAAGCTTG 
GATGTGGCCA TACAGGATAC ACGTCTCTCC CCGGGAATCC CGGATACCCC TACCCGTCTG 
GTATTCTCAG CTCTGGGACC CACCTCCCTA AAGGTCAGTT GGCAGGAGCC GCAATGCGAA 
AGAGAAGTTC TGGGATACAA AGTGCATTAT CAACTACTGC ATGGAGGTGA ATTAAGGACC 
ATCGACATTA CAAACCCACG GGAGAACTCA GTAGTGGTGG AAGACTTGCT TCCTAACCAC 
TCGTACATGT TCCGTGTGAA GGCACAAAGC GTTGAGGGCT GGGGGCCCGA AAGAGAAGGC 
GTTATCACTA TCGAATCACA GGTGGATCCT CAGAGTCCAC TCAGCCCTGT TCCAGGGTCT 
CCATTCACCC TAAGCACCCC CAGTGCTCCT GGACCACTGG TTTTCACAGC TCTGGCCCCT 
GACTCCCTGC AGTTAAGTTG GGTGAGACCC CGAAAGCCTA ATGGAACAAT TCTGGGGTAC 
ATGATCACTT GCGAGATGTT GCATGGAGGA GGGGAACCAC GAACGATCTA TGTTGAAGGT 
GACAAACCCG AGACTTCCCT GACGGTTCCT TTCCTAAATG AAAATCTGCC CTATAAATTT 
AAGGTCCAGG CGAAAACCAC ACAGGGTTTT GGACCAGAAC GGGAAGGGAT AATTACTATT 
GAATCTCAAG ATGGAGGAAA CTTTTCTCAG TTTGGAACAC AACAGATCAC ACGCCGAGAG 
GTGTTCAATA TGCCAGGAGA ATATACTCAA AGCAGCATCA CTCAATCCAA CATAACAGAA 
CCCTTCTTTT CAGATGGAAT GAGTGTCACC ACCCGCCGTA TCGAGGGCTC CAACACAGTC 
ACACAACAGG TCACCAAAGA ATTTGTCACA CGGACCATGA TGTCTGGCGG CTCCCTCAAC 
AGGCAAGTGG AGCGCCAGTT TTACGACGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itgb4 ( XM_002939974.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940020.1 Xenopus tropicalis integrin subunit beta 4 (itgb4), transcript variant X1, mRNA.

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CloneID	OXa17190
Clone ID Related Accession (Same CDS sequence)	XM_002939974.4 , XM_018097872.1
Accession Version	XM_002939974.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	integrin beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002939974.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAACC CTCGTGTTTT AGGGGGTCTC CTCCTGCTTG TCCTGCTCTT GGGTCTCCCA 
GCTTCCCATT CTCAGAGCAA CAAATATAAC CCCTGCGTGG CCTCCAGAGC AACCAGTTGC 
TCGGCCTGTA TCCGGGCGTA CAAGGACTGT TCCTACTGTG CAGATGAGGG TTTCGCTAGT 
GTACGCTGTG ATCTAGCAGA GAATCTGAGA AGATATGGCT GCAGCGAGAG AGGGATTGTT 
TACATGAGAA GTGAAGTCTT GACCCAGCAG AGCTATGACA TTAACACTCT CGTCAGCAAA 
ACCCAAGTGG CCCCTCAAAG GATGTCCATG CGTCTTCGTG CTGGGGAAGA AACCTCATTT 
GACCTGAATG TGTTTGAGCC GCTGGACACC CCTGTGGATT TGTACATTTT GATGGACTTC 
TCTCATTCCA TGTCAGATGA TTTAGCCAAC CTAAAAAATA TGGGTCAGGA GTTAGCTAAT 
GTTGTAAGCA CTCTCTCCAA GAATTACACC ATTGGATTTG GAAAATTTGT GGACAAAGTG 
ACTGTACCAC AGACTGACAT GAGACCTGAG AAACTGAAGC AACCCTGGTT GGACAGCACT 
CCTCCTTTCT CCTTCAAGAA CGTTATTCAA CTGACAAGCG ACGTGGGGAA ATTTCGGAAT 
GAATTGATGA AAGAGAAAAT CTCTGGAAAC CTTGATCCTC CAGAGGGGGG GTTTGATGCC 
ATTCTTCAGA CTGCTGTGTG CACGAATCAA ATTGGCTGGA GAAAAGGAAG TACTCATCTG 
TTGGTCTTCT CTACTGAGTC AGCATTCCAT TATGAAGCTG ATGGGATGAA CGTACTTGAT 
GGGATACTGA AGCGAAATGA TGAGCAGTGC CACTTGGATG CCTCTGGTTA CTACACACAC 
GACATCGTCC AGGACTATCC ATCTGTGCCC ACACTTGTTC GACTGTTAGC AAAGAACAAC 
ATCATCCCTA TCTTTGCCGT CACCAACTAC TCCTACAGTT ACTATGATAA GCTGAGCAAG 
TATTTCAAAG TATCTGAGAT TGGAGAATTG AAAGAAGATT CCTCCAACAT TGTGGAGCTC 
TTGCAGGAGG CCTTTACGCA AATTCGTTCC AGGATGGATA TCCGAGATGA TAACACCCCA 
CGTGCAATGG ATGTCTCAGT CACTTCTGAG ACAGCTAAAG TTACAGAAAC AGGATCGTTT 
CAAGTCACCC GGGGAGAAGT GGGTACCTTC AAAGTGAAGG TCAAGGCTTT AGAACGGTTG 
GGCAACCAGC ATGTGTGTGA ACTACCACCA CAAGACCAAG GGAGCCAAAT TCTCCTCAAA 
CCTTCCACCT TTACTGATGG CCTTAAGATT GATGCTTCTC TGATCTGTGA CAGCTGTCCT 
TGTGAATTGC AAAAGGAGTT GAGTTCCCCC AAGTGCAGCT TTAATGGCGA CTTTGTGTGC 
GGACGCTGTG AGTGCAGGGA GCGCTGGAGG GGAGTATATT GTAACTGCTC CAGCACAGAC 
ACCAAAAGCA CCCAATCGTG TATATCACCA GGCAGTACAG AGATTTGCTC CGGCAGGGGT 
GAATGCCTAT GCGGCACCTG TCAGTGTCAC TCGGAGTCCC CCACTCAACA GTACCAGGGG 
GAGTTCTGCC AGTTCAATAA CTTCCAGTGC CCACGTTTTC TTGGATACAT GTGCAATGAC 
AGAGGGCGCT GTCACATGGG AGCCTGTGTG TGTGATGCTG GCTGGGAAGG AGAGAGTTGT 
CAGTGTCCTA CCAGCAACCA AACCTGCCTT GATAGGAATG GGGGTCTGTG CAGTAGCCGT 
GGGAAGTGCG TGTGTGGGCG CTGCCAGTGT GATAACGTGT CACAGTATAC AGACGCCACG 
TGTGAATTCT CCTATTCACT GCGCCAACTG GGTGTGTGCG AAGACCTGCG GACGTGTGTG 
CAGTGCCAGG CCTGGGGAAC TGGAGAGAAA AAGGGCACAC AATGTGAAGC TTGTGAAATT 
AAGATTAAAA TGGTGGATGA GCTGAAGAAA GTGGATGAAA GAAGTGAAAA CTGTACTTTC 
CGGGATGAAA CAGATGACTG TACATATGAG TACTCAGTAG ACCCTCACGC AGTGCAAGGA 
AACTACACTG TGCTGGTCAA GAACAAAAAA GAATGTCCTC CTGGAGCCTT CCTCTGGCTT 
ATCCCTCTAC TCATCTTTCT GATGCTTCTC CTTGGGCTCT TGTTGCTCCT GTGCTGGAAA 
TACTGTGCCT GTTGCAAGGC TTGCTTGGCA ATGCTTCCCT GCTGTGGACC AGGACGGACA 
GTGGGATTTA AAGAAGACCA CTACATGTTA AGGCAAAGCC TGATGTCATC TGATTACCTG 
AATACCCCAC TGGTGCGTTC TGGGAGCCTC AAGGGCGGGG ACACTGTAAG ATGGAAGATC 
GACAACAACG TGCACCGCCA ACCACCCGCC CCTGGAACAC TGAACCCCAA GGAGCTTGTC 
CCATATGGCG TCTCACTGCG TCTGGCCCGG CTGTTCACTG AGAACCTGTC CAAGCCTCAA 
TCCCGTGAGT GTACCCAGCT GCGTCAGGAG GTGGAGGATT CGTTAAATGA GGTGTACAAG 
GTGATACCTG GAACCCACAA GGTGAAACAG ACAGCTTTCA GGCTTCAGTC CAATGCAGGA 
AAAAGACAGG ATCACACTAT TGCAGAAACC ATCCTGATGG CTCCCCGGGA GGCTGAACCT 
GAGATTGTAA AGGTTACAGA GAAACATGTA ACACAAGAGG CTTTCCAGGA TATGAAGGTT 
GCCCCAGGTT ACTATACAGT GACGTCAGAC AGAGATGCCC ATGGACTTGT AGAGTTCCAG 
GAGGGAGTTG AATTGGTGGA TGTCCGGGTT CCTCTCTTCA TCAGGGAAGA GGATGATGAT 
GAGAAGCAGT TGTTAGTGGA AGCTCTGGAC GTTCCATCAG GAATTGCCCG TTTGGGGCGG 
AAACTGGTCA ATATTACTAT TATCAAAGAG CAAGCCAAGA GCATAGTGAC GTTTGTGAAA 
CCTGCCTACA CCTACAGCCG ACAGGAACAG GTTGCCCGTG TCCCAGTTTC TCGTGAGATC 
ATCCAGAATG GAAAGACACA AGTTACATAT CGTTCCAAGG ACCTGACTGC TAGGGAGGGA 
AAGGACTATA CATTCTCAGA GGGGGAGCTC ACCTTCCAGC CAGATGACAC TCAATCAGAG 
ATTCTAGTGC CTATACTCGG TCGTTCAGAA GTTGACACTC TGCTGGGAAA TAAACAAGTC 
AAACAGTTCC TTCTAGAACT CAGTAATCCT AAGTATGGGG CCAAGATTGG CAAATACCCA 
CAATGCACCA TCACTATTGA TGACACTGCA GATGTTATAC AAAAGACAAT GACAGTCCAG 
TCTGTTCAGT CCCCCCGTGG GAAGGTAGGT GCTCCTATCA ACCTGAATGC CCAGGCCCTC 
AGCGCCAGGA AAATCCGACT CAACTGGATG CCGCCTCCTG GAAAACCCAC AGGTTACAAG 
GTGCGTTACT GGATTCAAGG AGATTCCGAG TCAGATGCGA CTGTGGTGGA TACAAATGTG 
CCCTCTGCAG AGCTGGTCAA TCTGTACCCC TACTGTGACT ACGAGATGCG GGTATGCGCA 
TACAATGCTC AAGAGGAGGG TCCCTTCACT GACATAGTCA CCTGCCAGAC CTTGGAGGAA 
GCACCCAGTG AACCCGGCCG CCTCGCCTTT AACGTCATTT CAAGCACAGT GACGCAGCTG 
AGTTGGGCAG AGCCAGCTGA AACCAATGGT GAAATCACAG CGTATGAGGT CACCTACGGA 
ATGGTCAACG AGGACAACAA ACCCATTGGG CCCACGAAGA AAGTCCTGAT TAATGATTCA 
AAGAAGCGCA TGGTTCTAAT TGAAAACCTA CGAGAATCCC AGCCATACCG ATACACTGTG 
AGAGCCTGCA ACAAAGCTGG GTGGGGGCCA GAGAGAGATG CCACCATGAA CCTAGCTACC 
CAACCCACCC GCCCAATGTC TATTCCCATT ATCCCTGATG CTCCCATTAT TGATGCTGAA 
GCTGGGGAAG AATATGACAG TTACCTGATG TACAGCACCG ATGTCCTGCG CTCTCCAGTT 
GGCAGCAAGA GACCCAGTGT GTCCGACGAG TCAGGCCAGC GCTGGAAGTA TGTCCCTCTC 
CTGGGCTCGG AGATGGACCT GCGGCGGGTC ACCTGGAAGC TACCCGTAGA GGTCATACCA 
CGCCTGTCCG TCAGCAGCGG CCTGTCCACT GACAACGAGA GCTTGCTGGA CCAGCCTTTG 
ACTACCAGCT CACTGACGAG GAAAGGGACC ATGCGAGCAA CCCCAGAAGA GCAATTAATT 
AACGGACGCA CAGAATTCTC CTTCTCGGGC GGCAGTCTCA ACCGTTCCAT GGTATCAGGG 
TACCAGCAAC TCAGCCCGCA CGTGCAAAAT GAAAGCTACA GGTTTGGTAG CAGCAGTTCC 
CAGCATACAT CCACAACGGT CATATCAGGG CAAGGCTCAT CAGGGCAGAA CAGACTGGGG 
CTCCCCCCAT TGGTGTTAGG AGGAGGAAGG GGACGAACCC ACTCAGAGGA TGTGAGGGAT 
GCTCTGACAA GCTTGGATGT GGCCATACAG GATACACGTC TCTCCCCGGG AATCCCGGAT 
ACCCCTACCC GTCTGGTATT CTCAGCTCTG GGACCCACCT CCCTAAAGGT CAGTTGGCAG 
GAGCCGCAAT GCGAAAGAGA AGTTCTGGGA TACAAAGTGC ATTATCAACT ACTGCATGGA 
GGTGAATTAA GGACCATCGA CATTACAAAC CCACGGGAGA ACTCAGTAGT GGTGGAAGAC 
TTGCTTCCTA ACCACTCGTA CATGTTCCGT GTGAAGGCAC AAAGCGTTGA GGGCTGGGGG 
CCCGAAAGAG AAGGCGTTAT CACTATCGAA TCACAGGTGG ATCCTCAGAG TCCACTCAGC 
CCTGTTCCAG GGTCTCCATT CACCCTAAGC ACCCCCAGTG CTCCTGGACC ACTGGTTTTC 
ACAGCTCTGG CCCCTGACTC CCTGCAGTTA AGTTGGGTGA GACCCCGAAA GCCTAATGGA 
ACAATTCTGG GGTACATGAT CACTTGCGAG ATGTTGCATG GAGGAGGGGA ACCACGAACG 
ATCTATGTTG AAGGTGACAA ACCCGAGACT TCCCTGACGG TTCCTTTCCT AAATGAAAAT 
CTGCCCTATA AATTTAAGGT CCAGGCGAAA ACCACACAGG GTTTTGGACC AGAACGGGAA 
GGGATAATTA CTATTGAATC TCAAGATGGA GGAAACTTTT CTCAGTTTGG AACACAACAG 
ATCACACGCC GAGAGGTGTT CAATATGCCA GGAGAATATA CTCAAAGCAG CATCACTCAA 
TCCAACATAA CAGAACCCTT CTTTTCAGAT GGAATGAGTG TCACCACCCG CCGTATCGAG 
GGCTCCAACA CAGTCACACA ACAGGTCACC AAAGAATTTG TCACACGGAC CATGATGTCT 
GGCGGCTCCC TCAACAGGCA AGTGGAGCGC CAGTTTTACG ACGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940020.1
CDS	1130..6637
Translation	MMNPRVLGGLLLLVLLLGLPASHSQSNKYNPCVASRATSCSACIRAYKDCSYCADEGFASVRCDLAENLRRYGCSERGIVYMRSEVLTQQSYDINTLVSKTQVAPQRMSMRLRAGEETSFDLNVFEPLDTPVDLYILMDFSHSMSDDLANLKNMGQELANVVSTLSKNYTIGFGKFVDKVTVPQTDMRPEKLKQPWLDSTPPFSFKNVIQLTSDVGKFRNELMKEKISGNLDPPEGGFDAILQTAVCTNQIGWRKGSTHLLVFSTESAFHYEADGMNVLDGILKRNDEQCHLDASGYYTHDIVQDYPSVPTLVRLLAKNNIIPIFAVTNYSYSYYDKLSKYFKVSEIGELKEDSSNIVELLQEAFTQIRSRMDIRDDNTPRAMDVSVTSETAKVTETGSFQVTRGEVGTFKVKVKALERLGNQHVCELPPQDQGSQILLKPSTFTDGLKIDASLICDSCPCELQKELSSPKCSFNGDFVCGRCECRERWRGVYCNCSSTDTKSTQSCISPGSTEICSGRGECLCGTCQCHSESPTQQYQGEFCQFNNFQCPRFLGYMCNDRGRCHMGACVCDAGWEGESCQCPTSNQTCLDRNGGLCSSRGKCVCGRCQCDNVSQYTDATCEFSYSLRQLGVCEDLRTCVQCQAWGTGEKKGTQCEACEIKIKMVDELKKVDERSENCTFRDETDDCTYEYSVDPHAVQGNYTVLVKNKKECPPGAFLWLIPLLIFLMLLLGLLLLLCWKYCACCKACLAMLPCCGPGRTVGFKEDHYMLRQSLMSSDYLNTPLVRSGSLKGGDTVRWKIDNNVHRQPPAPGTLNPKELVPYGVSLRLARLFTENLSKPQSRECTQLRQEVEDSLNEVYKVIPGTHKVKQTAFRLQSNAGKRQDHTIAETILMAPREAEPEIVKVTEKHVTQEAFQDMKVAPGYYTVTSDRDAHGLVEFQEGVELVDVRVPLFIREEDDDEKQLLVEALDVPSGIARLGRKLVNITIIKEQAKSIVTFVKPAYTYSRQEQVARVPVSREIIQNGKTQVTYRSKDLTAREGKDYTFSEGELTFQPDDTQSEILVPILGRSEVDTLLGNKQVKQFLLELSNPKYGAKIGKYPQCTITIDDTADVIQKTMTVQSVQSPRGKVGAPINLNAQALSARKIRLNWMPPPGKPTGYKVRYWIQGDSESDATVVDTNVPSAELVNLYPYCDYEMRVCAYNAQEEGPFTDIVTCQTLEEAPSEPGRLAFNVISSTVTQLSWAEPAETNGEITAYEVTYGMVNEDNKPIGPTKKVLINDSKKRMVLIENLRESQPYRYTVRACNKAGWGPERDATMNLATQPTRPMSIPIIPDAPIIDAEAGEEYDSYLMYSTDVLRSPVGSKRPSVSDESGQRWKYVPLLGSEMDLRRVTWKLPVEVIPRLSVSSGLSTDNESLLDQPLTTSSLTRKGTMRATPEEQLINGRTEFSFSGGSLNRSMVSGYQQLSPHVQNESYRFGSSSSQHTSTTVISGQGSSGQNRLGLPPLVLGGGRGRTHSEDVRDALTSLDVAIQDTRLSPGIPDTPTRLVFSALGPTSLKVSWQEPQCEREVLGYKVHYQLLHGGELRTIDITNPRENSVVVEDLLPNHSYMFRVKAQSVEGWGPEREGVITIESQVDPQSPLSPVPGSPFTLSTPSAPGPLVFTALAPDSLQLSWVRPRKPNGTILGYMITCEMLHGGGEPRTIYVEGDKPETSLTVPFLNENLPYKFKVQAKTTQGFGPEREGIITIESQDGGNFSQFGTQQITRREVFNMPGEYTQSSITQSNITEPFFSDGMSVTTRRIEGSNTVTQQVTKEFVTRTMMSGGSLNRQVERQFYDA

Target ORF information:

RefSeq Version	XM_002939974.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis integrin subunit beta 4 (itgb4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939974.4

ORF Insert Sequence:

ATGATGAACC CTCGTGTTTT AGGGGGTCTC CTCCTGCTTG TCCTGCTCTT GGGTCTCCCA 
GCTTCCCATT CTCAGAGCAA CAAATATAAC CCCTGCGTGG CCTCCAGAGC AACCAGTTGC 
TCGGCCTGTA TCCGGGCGTA CAAGGACTGT TCCTACTGTG CAGATGAGGG TTTCGCTAGT 
GTACGCTGTG ATCTAGCAGA GAATCTGAGA AGATATGGCT GCAGCGAGAG AGGGATTGTT 
TACATGAGAA GTGAAGTCTT GACCCAGCAG AGCTATGACA TTAACACTCT CGTCAGCAAA 
ACCCAAGTGG CCCCTCAAAG GATGTCCATG CGTCTTCGTG CTGGGGAAGA AACCTCATTT 
GACCTGAATG TGTTTGAGCC GCTGGACACC CCTGTGGATT TGTACATTTT GATGGACTTC 
TCTCATTCCA TGTCAGATGA TTTAGCCAAC CTAAAAAATA TGGGTCAGGA GTTAGCTAAT 
GTTGTAAGCA CTCTCTCCAA GAATTACACC ATTGGATTTG GAAAATTTGT GGACAAAGTG 
ACTGTACCAC AGACTGACAT GAGACCTGAG AAACTGAAGC AACCCTGGTT GGACAGCACT 
CCTCCTTTCT CCTTCAAGAA CGTTATTCAA CTGACAAGCG ACGTGGGGAA ATTTCGGAAT 
GAATTGATGA AAGAGAAAAT CTCTGGAAAC CTTGATCCTC CAGAGGGGGG GTTTGATGCC 
ATTCTTCAGA CTGCTGTGTG CACGAATCAA ATTGGCTGGA GAAAAGGAAG TACTCATCTG 
TTGGTCTTCT CTACTGAGTC AGCATTCCAT TATGAAGCTG ATGGGATGAA CGTACTTGAT 
GGGATACTGA AGCGAAATGA TGAGCAGTGC CACTTGGATG CCTCTGGTTA CTACACACAC 
GACATCGTCC AGGACTATCC ATCTGTGCCC ACACTTGTTC GACTGTTAGC AAAGAACAAC 
ATCATCCCTA TCTTTGCCGT CACCAACTAC TCCTACAGTT ACTATGATAA GCTGAGCAAG 
TATTTCAAAG TATCTGAGAT TGGAGAATTG AAAGAAGATT CCTCCAACAT TGTGGAGCTC 
TTGCAGGAGG CCTTTACGCA AATTCGTTCC AGGATGGATA TCCGAGATGA TAACACCCCA 
CGTGCAATGG ATGTCTCAGT CACTTCTGAG ACAGCTAAAG TTACAGAAAC AGGATCGTTT 
CAAGTCACCC GGGGAGAAGT GGGTACCTTC AAAGTGAAGG TCAAGGCTTT AGAACGGTTG 
GGCAACCAGC ATGTGTGTGA ACTACCACCA CAAGACCAAG GGAGCCAAAT TCTCCTCAAA 
CCTTCCACCT TTACTGATGG CCTTAAGATT GATGCTTCTC TGATCTGTGA CAGCTGTCCT 
TGTGAATTGC AAAAGGAGTT GAGTTCCCCC AAGTGCAGCT TTAATGGCGA CTTTGTGTGC 
GGACGCTGTG AGTGCAGGGA GCGCTGGAGG GGAGTATATT GTAACTGCTC CAGCACAGAC 
ACCAAAAGCA CCCAATCGTG TATATCACCA GGCAGTACAG AGATTTGCTC CGGCAGGGGT 
GAATGCCTAT GCGGCACCTG TCAGTGTCAC TCGGAGTCCC CCACTCAACA GTACCAGGGG 
GAGTTCTGCC AGTTCAATAA CTTCCAGTGC CCACGTTTTC TTGGATACAT GTGCAATGAC 
AGAGGGCGCT GTCACATGGG AGCCTGTGTG TGTGATGCTG GCTGGGAAGG AGAGAGTTGT 
CAGTGTCCTA CCAGCAACCA AACCTGCCTT GATAGGAATG GGGGTCTGTG CAGTAGCCGT 
GGGAAGTGCG TGTGTGGGCG CTGCCAGTGT GATAACGTGT CACAGTATAC AGACGCCACG 
TGTGAATTCT CCTATTCACT GCGCCAACTG GGTGTGTGCG AAGACCTGCG GACGTGTGTG 
CAGTGCCAGG CCTGGGGAAC TGGAGAGAAA AAGGGCACAC AATGTGAAGC TTGTGAAATT 
AAGATTAAAA TGGTGGATGA GCTGAAGAAA GTGGATGAAA GAAGTGAAAA CTGTACTTTC 
CGGGATGAAA CAGATGACTG TACATATGAG TACTCAGTAG ACCCTCACGC AGTGCAAGGA 
AACTACACTG TGCTGGTCAA GAACAAAAAA GAATGTCCTC CTGGAGCCTT CCTCTGGCTT 
ATCCCTCTAC TCATCTTTCT GATGCTTCTC CTTGGGCTCT TGTTGCTCCT GTGCTGGAAA 
TACTGTGCCT GTTGCAAGGC TTGCTTGGCA ATGCTTCCCT GCTGTGGACC AGGACGGACA 
GTGGGATTTA AAGAAGACCA CTACATGTTA AGGCAAAGCC TGATGTCATC TGATTACCTG 
AATACCCCAC TGGTGCGTTC TGGGAGCCTC AAGGGCGGGG ACACTGTAAG ATGGAAGATC 
GACAACAACG TGCACCGCCA ACCACCCGCC CCTGGAACAC TGAACCCCAA GGAGCTTGTC 
CCATATGGCG TCTCACTGCG TCTGGCCCGG CTGTTCACTG AGAACCTGTC CAAGCCTCAA 
TCCCGTGAGT GTACCCAGCT GCGTCAGGAG GTGGAGGATT CGTTAAATGA GGTGTACAAG 
GTGATACCTG GAACCCACAA GGTGAAACAG ACAGCTTTCA GGCTTCAGTC CAATGCAGGA 
AAAAGACAGG ATCACACTAT TGCAGAAACC ATCCTGATGG CTCCCCGGGA GGCTGAACCT 
GAGATTGTAA AGGTTACAGA GAAACATGTA ACACAAGAGG CTTTCCAGGA TATGAAGGTT 
GCCCCAGGTT ACTATACAGT GACGTCAGAC AGAGATGCCC ATGGACTTGT AGAGTTCCAG 
GAGGGAGTTG AATTGGTGGA TGTCCGGGTT CCTCTCTTCA TCAGGGAAGA GGATGATGAT 
GAGAAGCAGT TGTTAGTGGA AGCTCTGGAC GTTCCATCAG GAATTGCCCG TTTGGGGCGG 
AAACTGGTCA ATATTACTAT TATCAAAGAG CAAGCCAAGA GCATAGTGAC GTTTGTGAAA 
CCTGCCTACA CCTACAGCCG ACAGGAACAG GTTGCCCGTG TCCCAGTTTC TCGTGAGATC 
ATCCAGAATG GAAAGACACA AGTTACATAT CGTTCCAAGG ACCTGACTGC TAGGGAGGGA 
AAGGACTATA CATTCTCAGA GGGGGAGCTC ACCTTCCAGC CAGATGACAC TCAATCAGAG 
ATTCTAGTGC CTATACTCGG TCGTTCAGAA GTTGACACTC TGCTGGGAAA TAAACAAGTC 
AAACAGTTCC TTCTAGAACT CAGTAATCCT AAGTATGGGG CCAAGATTGG CAAATACCCA 
CAATGCACCA TCACTATTGA TGACACTGCA GATGTTATAC AAAAGACAAT GACAGTCCAG 
TCTGTTCAGT CCCCCCGTGG GAAGGTAGGT GCTCCTATCA ACCTGAATGC CCAGGCCCTC 
AGCGCCAGGA AAATCCGACT CAACTGGATG CCGCCTCCTG GAAAACCCAC AGGTTACAAG 
GTGCGTTACT GGATTCAAGG AGATTCCGAG TCAGATGCGA CTGTGGTGGA TACAAATGTG 
CCCTCTGCAG AGCTGGTCAA TCTGTACCCC TACTGTGACT ACGAGATGCG GGTATGCGCA 
TACAATGCTC AAGAGGAGGG TCCCTTCACT GACATAGTCA CCTGCCAGAC CTTGGAGGAA 
GCACCCAGTG AACCCGGCCG CCTCGCCTTT AACGTCATTT CAAGCACAGT GACGCAGCTG 
AGTTGGGCAG AGCCAGCTGA AACCAATGGT GAAATCACAG CGTATGAGGT CACCTACGGA 
ATGGTCAACG AGGACAACAA ACCCATTGGG CCCACGAAGA AAGTCCTGAT TAATGATTCA 
AAGAAGCGCA TGGTTCTAAT TGAAAACCTA CGAGAATCCC AGCCATACCG ATACACTGTG 
AGAGCCTGCA ACAAAGCTGG GTGGGGGCCA GAGAGAGATG CCACCATGAA CCTAGCTACC 
CAACCCACCC GCCCAATGTC TATTCCCATT ATCCCTGATG CTCCCATTAT TGATGCTGAA 
GCTGGGGAAG AATATGACAG TTACCTGATG TACAGCACCG ATGTCCTGCG CTCTCCAGTT 
GGCAGCAAGA GACCCAGTGT GTCCGACGAG TCAGGCCAGC GCTGGAAGTA TGTCCCTCTC 
CTGGGCTCGG AGATGGACCT GCGGCGGGTC ACCTGGAAGC TACCCGTAGA GGTCATACCA 
CGCCTGTCCG TCAGCAGCGG CCTGTCCACT GACAACGAGA GCTTGCTGGA CCAGCCTTTG 
ACTACCAGCT CACTGACGAG GAAAGGGACC ATGCGAGCAA CCCCAGAAGA GCAATTAATT 
AACGGACGCA CAGAATTCTC CTTCTCGGGC GGCAGTCTCA ACCGTTCCAT GGTATCAGGG 
TACCAGCAAC TCAGCCCGCA CGTGCAAAAT GAAAGCTACA GGTTTGGTAG CAGCAGTTCC 
CAGCATACAT CCACAACGGT CATATCAGGG CAAGGCTCAT CAGGGCAGAA CAGACTGGGG 
CTCCCCCCAT TGGTGTTAGG AGGAGGAAGG GGACGAACCC ACTCAGAGGA TGTGAGGGAT 
GCTCTGACAA GCTTGGATGT GGCCATACAG GATACACGTC TCTCCCCGGG AATCCCGGAT 
ACCCCTACCC GTCTGGTATT CTCAGCTCTG GGACCCACCT CCCTAAAGGT CAGTTGGCAG 
GAGCCGCAAT GCGAAAGAGA AGTTCTGGGA TACAAAGTGC ATTATCAACT ACTGCATGGA 
GGTGAATTAA GGACCATCGA CATTACAAAC CCACGGGAGA ACTCAGTAGT GGTGGAAGAC 
TTGCTTCCTA ACCACTCGTA CATGTTCCGT GTGAAGGCAC AAAGCGTTGA GGGCTGGGGG 
CCCGAAAGAG AAGGCGTTAT CACTATCGAA TCACAGGTGG ATCCTCAGAG TCCACTCAGC 
CCTGTTCCAG GGTCTCCATT CACCCTAAGC ACCCCCAGTG CTCCTGGACC ACTGGTTTTC 
ACAGCTCTGG CCCCTGACTC CCTGCAGTTA AGTTGGGTGA GACCCCGAAA GCCTAATGGA 
ACAATTCTGG GGTACATGAT CACTTGCGAG ATGTTGCATG GAGGAGGGGA ACCACGAACG 
ATCTATGTTG AAGGTGACAA ACCCGAGACT TCCCTGACGG TTCCTTTCCT AAATGAAAAT 
CTGCCCTATA AATTTAAGGT CCAGGCGAAA ACCACACAGG GTTTTGGACC AGAACGGGAA 
GGGATAATTA CTATTGAATC TCAAGATGGA GGAAACTTTT CTCAGTTTGG AACACAACAG 
ATCACACGCC GAGAGGTGTT CAATATGCCA GGAGAATATA CTCAAAGCAG CATCACTCAA 
TCCAACATAA CAGAACCCTT CTTTTCAGAT GGAATGAGTG TCACCACCCG CCGTATCGAG 
GGCTCCAACA CAGTCACACA ACAGGTCACC AAAGAATTTG TCACACGGAC CATGATGTCT 
GGCGGCTCCC TCAACAGGCA AGTGGAGCGC CAGTTTTACG ACGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itgb4 ( XM_018097872.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017953361.1 Xenopus tropicalis integrin subunit beta 4 (itgb4), transcript variant X2, mRNA.

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CloneID	OXa17190
Clone ID Related Accession (Same CDS sequence)	XM_002939974.4 , XM_018097872.1
Accession Version	XM_018097872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	integrin beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAACC CTCGTGTTTT AGGGGGTCTC CTCCTGCTTG TCCTGCTCTT GGGTCTCCCA 
GCTTCCCATT CTCAGAGCAA CAAATATAAC CCCTGCGTGG CCTCCAGAGC AACCAGTTGC 
TCGGCCTGTA TCCGGGCGTA CAAGGACTGT TCCTACTGTG CAGATGAGGG TTTCGCTAGT 
GTACGCTGTG ATCTAGCAGA GAATCTGAGA AGATATGGCT GCAGCGAGAG AGGGATTGTT 
TACATGAGAA GTGAAGTCTT GACCCAGCAG AGCTATGACA TTAACACTCT CGTCAGCAAA 
ACCCAAGTGG CCCCTCAAAG GATGTCCATG CGTCTTCGTG CTGGGGAAGA AACCTCATTT 
GACCTGAATG TGTTTGAGCC GCTGGACACC CCTGTGGATT TGTACATTTT GATGGACTTC 
TCTCATTCCA TGTCAGATGA TTTAGCCAAC CTAAAAAATA TGGGTCAGGA GTTAGCTAAT 
GTTGTAAGCA CTCTCTCCAA GAATTACACC ATTGGATTTG GAAAATTTGT GGACAAAGTG 
ACTGTACCAC AGACTGACAT GAGACCTGAG AAACTGAAGC AACCCTGGTT GGACAGCACT 
CCTCCTTTCT CCTTCAAGAA CGTTATTCAA CTGACAAGCG ACGTGGGGAA ATTTCGGAAT 
GAATTGATGA AAGAGAAAAT CTCTGGAAAC CTTGATCCTC CAGAGGGGGG GTTTGATGCC 
ATTCTTCAGA CTGCTGTGTG CACGAATCAA ATTGGCTGGA GAAAAGGAAG TACTCATCTG 
TTGGTCTTCT CTACTGAGTC AGCATTCCAT TATGAAGCTG ATGGGATGAA CGTACTTGAT 
GGGATACTGA AGCGAAATGA TGAGCAGTGC CACTTGGATG CCTCTGGTTA CTACACACAC 
GACATCGTCC AGGACTATCC ATCTGTGCCC ACACTTGTTC GACTGTTAGC AAAGAACAAC 
ATCATCCCTA TCTTTGCCGT CACCAACTAC TCCTACAGTT ACTATGATAA GCTGAGCAAG 
TATTTCAAAG TATCTGAGAT TGGAGAATTG AAAGAAGATT CCTCCAACAT TGTGGAGCTC 
TTGCAGGAGG CCTTTACGCA AATTCGTTCC AGGATGGATA TCCGAGATGA TAACACCCCA 
CGTGCAATGG ATGTCTCAGT CACTTCTGAG ACAGCTAAAG TTACAGAAAC AGGATCGTTT 
CAAGTCACCC GGGGAGAAGT GGGTACCTTC AAAGTGAAGG TCAAGGCTTT AGAACGGTTG 
GGCAACCAGC ATGTGTGTGA ACTACCACCA CAAGACCAAG GGAGCCAAAT TCTCCTCAAA 
CCTTCCACCT TTACTGATGG CCTTAAGATT GATGCTTCTC TGATCTGTGA CAGCTGTCCT 
TGTGAATTGC AAAAGGAGTT GAGTTCCCCC AAGTGCAGCT TTAATGGCGA CTTTGTGTGC 
GGACGCTGTG AGTGCAGGGA GCGCTGGAGG GGAGTATATT GTAACTGCTC CAGCACAGAC 
ACCAAAAGCA CCCAATCGTG TATATCACCA GGCAGTACAG AGATTTGCTC CGGCAGGGGT 
GAATGCCTAT GCGGCACCTG TCAGTGTCAC TCGGAGTCCC CCACTCAACA GTACCAGGGG 
GAGTTCTGCC AGTTCAATAA CTTCCAGTGC CCACGTTTTC TTGGATACAT GTGCAATGAC 
AGAGGGCGCT GTCACATGGG AGCCTGTGTG TGTGATGCTG GCTGGGAAGG AGAGAGTTGT 
CAGTGTCCTA CCAGCAACCA AACCTGCCTT GATAGGAATG GGGGTCTGTG CAGTAGCCGT 
GGGAAGTGCG TGTGTGGGCG CTGCCAGTGT GATAACGTGT CACAGTATAC AGACGCCACG 
TGTGAATTCT CCTATTCACT GCGCCAACTG GGTGTGTGCG AAGACCTGCG GACGTGTGTG 
CAGTGCCAGG CCTGGGGAAC TGGAGAGAAA AAGGGCACAC AATGTGAAGC TTGTGAAATT 
AAGATTAAAA TGGTGGATGA GCTGAAGAAA GTGGATGAAA GAAGTGAAAA CTGTACTTTC 
CGGGATGAAA CAGATGACTG TACATATGAG TACTCAGTAG ACCCTCACGC AGTGCAAGGA 
AACTACACTG TGCTGGTCAA GAACAAAAAA GAATGTCCTC CTGGAGCCTT CCTCTGGCTT 
ATCCCTCTAC TCATCTTTCT GATGCTTCTC CTTGGGCTCT TGTTGCTCCT GTGCTGGAAA 
TACTGTGCCT GTTGCAAGGC TTGCTTGGCA ATGCTTCCCT GCTGTGGACC AGGACGGACA 
GTGGGATTTA AAGAAGACCA CTACATGTTA AGGCAAAGCC TGATGTCATC TGATTACCTG 
AATACCCCAC TGGTGCGTTC TGGGAGCCTC AAGGGCGGGG ACACTGTAAG ATGGAAGATC 
GACAACAACG TGCACCGCCA ACCACCCGCC CCTGGAACAC TGAACCCCAA GGAGCTTGTC 
CCATATGGCG TCTCACTGCG TCTGGCCCGG CTGTTCACTG AGAACCTGTC CAAGCCTCAA 
TCCCGTGAGT GTACCCAGCT GCGTCAGGAG GTGGAGGATT CGTTAAATGA GGTGTACAAG 
GTGATACCTG GAACCCACAA GGTGAAACAG ACAGCTTTCA GGCTTCAGTC CAATGCAGGA 
AAAAGACAGG ATCACACTAT TGCAGAAACC ATCCTGATGG CTCCCCGGGA GGCTGAACCT 
GAGATTGTAA AGGTTACAGA GAAACATGTA ACACAAGAGG CTTTCCAGGA TATGAAGGTT 
GCCCCAGGTT ACTATACAGT GACGTCAGAC AGAGATGCCC ATGGACTTGT AGAGTTCCAG 
GAGGGAGTTG AATTGGTGGA TGTCCGGGTT CCTCTCTTCA TCAGGGAAGA GGATGATGAT 
GAGAAGCAGT TGTTAGTGGA AGCTCTGGAC GTTCCATCAG GAATTGCCCG TTTGGGGCGG 
AAACTGGTCA ATATTACTAT TATCAAAGAG CAAGCCAAGA GCATAGTGAC GTTTGTGAAA 
CCTGCCTACA CCTACAGCCG ACAGGAACAG GTTGCCCGTG TCCCAGTTTC TCGTGAGATC 
ATCCAGAATG GAAAGACACA AGTTACATAT CGTTCCAAGG ACCTGACTGC TAGGGAGGGA 
AAGGACTATA CATTCTCAGA GGGGGAGCTC ACCTTCCAGC CAGATGACAC TCAATCAGAG 
ATTCTAGTGC CTATACTCGG TCGTTCAGAA GTTGACACTC TGCTGGGAAA TAAACAAGTC 
AAACAGTTCC TTCTAGAACT CAGTAATCCT AAGTATGGGG CCAAGATTGG CAAATACCCA 
CAATGCACCA TCACTATTGA TGACACTGCA GATGTTATAC AAAAGACAAT GACAGTCCAG 
TCTGTTCAGT CCCCCCGTGG GAAGGTAGGT GCTCCTATCA ACCTGAATGC CCAGGCCCTC 
AGCGCCAGGA AAATCCGACT CAACTGGATG CCGCCTCCTG GAAAACCCAC AGGTTACAAG 
GTGCGTTACT GGATTCAAGG AGATTCCGAG TCAGATGCGA CTGTGGTGGA TACAAATGTG 
CCCTCTGCAG AGCTGGTCAA TCTGTACCCC TACTGTGACT ACGAGATGCG GGTATGCGCA 
TACAATGCTC AAGAGGAGGG TCCCTTCACT GACATAGTCA CCTGCCAGAC CTTGGAGGAA 
GCACCCAGTG AACCCGGCCG CCTCGCCTTT AACGTCATTT CAAGCACAGT GACGCAGCTG 
AGTTGGGCAG AGCCAGCTGA AACCAATGGT GAAATCACAG CGTATGAGGT CACCTACGGA 
ATGGTCAACG AGGACAACAA ACCCATTGGG CCCACGAAGA AAGTCCTGAT TAATGATTCA 
AAGAAGCGCA TGGTTCTAAT TGAAAACCTA CGAGAATCCC AGCCATACCG ATACACTGTG 
AGAGCCTGCA ACAAAGCTGG GTGGGGGCCA GAGAGAGATG CCACCATGAA CCTAGCTACC 
CAACCCACCC GCCCAATGTC TATTCCCATT ATCCCTGATG CTCCCATTAT TGATGCTGAA 
GCTGGGGAAG AATATGACAG TTACCTGATG TACAGCACCG ATGTCCTGCG CTCTCCAGTT 
GGCAGCAAGA GACCCAGTGT GTCCGACGAG TCAGGCCAGC GCTGGAAGTA TGTCCCTCTC 
CTGGGCTCGG AGATGGACCT GCGGCGGGTC ACCTGGAAGC TACCCGTAGA GGTCATACCA 
CGCCTGTCCG TCAGCAGCGG CCTGTCCACT GACAACGAGA GCTTGCTGGA CCAGCCTTTG 
ACTACCAGCT CACTGACGAG GAAAGGGACC ATGCGAGCAA CCCCAGAAGA GCAATTAATT 
AACGGACGCA CAGAATTCTC CTTCTCGGGC GGCAGTCTCA ACCGTTCCAT GGTATCAGGG 
TACCAGCAAC TCAGCCCGCA CGTGCAAAAT GAAAGCTACA GGTTTGGTAG CAGCAGTTCC 
CAGCATACAT CCACAACGGT CATATCAGGG CAAGGCTCAT CAGGGCAGAA CAGACTGGGG 
CTCCCCCCAT TGGTGTTAGG AGGAGGAAGG GGACGAACCC ACTCAGAGGA TGTGAGGGAT 
GCTCTGACAA GCTTGGATGT GGCCATACAG GATACACGTC TCTCCCCGGG AATCCCGGAT 
ACCCCTACCC GTCTGGTATT CTCAGCTCTG GGACCCACCT CCCTAAAGGT CAGTTGGCAG 
GAGCCGCAAT GCGAAAGAGA AGTTCTGGGA TACAAAGTGC ATTATCAACT ACTGCATGGA 
GGTGAATTAA GGACCATCGA CATTACAAAC CCACGGGAGA ACTCAGTAGT GGTGGAAGAC 
TTGCTTCCTA ACCACTCGTA CATGTTCCGT GTGAAGGCAC AAAGCGTTGA GGGCTGGGGG 
CCCGAAAGAG AAGGCGTTAT CACTATCGAA TCACAGGTGG ATCCTCAGAG TCCACTCAGC 
CCTGTTCCAG GGTCTCCATT CACCCTAAGC ACCCCCAGTG CTCCTGGACC ACTGGTTTTC 
ACAGCTCTGG CCCCTGACTC CCTGCAGTTA AGTTGGGTGA GACCCCGAAA GCCTAATGGA 
ACAATTCTGG GGTACATGAT CACTTGCGAG ATGTTGCATG GAGGAGGGGA ACCACGAACG 
ATCTATGTTG AAGGTGACAA ACCCGAGACT TCCCTGACGG TTCCTTTCCT AAATGAAAAT 
CTGCCCTATA AATTTAAGGT CCAGGCGAAA ACCACACAGG GTTTTGGACC AGAACGGGAA 
GGGATAATTA CTATTGAATC TCAAGATGGA GGAAACTTTT CTCAGTTTGG AACACAACAG 
ATCACACGCC GAGAGGTGTT CAATATGCCA GGAGAATATA CTCAAAGCAG CATCACTCAA 
TCCAACATAA CAGAACCCTT CTTTTCAGAT GGAATGAGTG TCACCACCCG CCGTATCGAG 
GGCTCCAACA CAGTCACACA ACAGGTCACC AAAGAATTTG TCACACGGAC CATGATGTCT 
GGCGGCTCCC TCAACAGGCA AGTGGAGCGC CAGTTTTACG ACGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017953361.1
CDS	1251..6758
Translation	MMNPRVLGGLLLLVLLLGLPASHSQSNKYNPCVASRATSCSACIRAYKDCSYCADEGFASVRCDLAENLRRYGCSERGIVYMRSEVLTQQSYDINTLVSKTQVAPQRMSMRLRAGEETSFDLNVFEPLDTPVDLYILMDFSHSMSDDLANLKNMGQELANVVSTLSKNYTIGFGKFVDKVTVPQTDMRPEKLKQPWLDSTPPFSFKNVIQLTSDVGKFRNELMKEKISGNLDPPEGGFDAILQTAVCTNQIGWRKGSTHLLVFSTESAFHYEADGMNVLDGILKRNDEQCHLDASGYYTHDIVQDYPSVPTLVRLLAKNNIIPIFAVTNYSYSYYDKLSKYFKVSEIGELKEDSSNIVELLQEAFTQIRSRMDIRDDNTPRAMDVSVTSETAKVTETGSFQVTRGEVGTFKVKVKALERLGNQHVCELPPQDQGSQILLKPSTFTDGLKIDASLICDSCPCELQKELSSPKCSFNGDFVCGRCECRERWRGVYCNCSSTDTKSTQSCISPGSTEICSGRGECLCGTCQCHSESPTQQYQGEFCQFNNFQCPRFLGYMCNDRGRCHMGACVCDAGWEGESCQCPTSNQTCLDRNGGLCSSRGKCVCGRCQCDNVSQYTDATCEFSYSLRQLGVCEDLRTCVQCQAWGTGEKKGTQCEACEIKIKMVDELKKVDERSENCTFRDETDDCTYEYSVDPHAVQGNYTVLVKNKKECPPGAFLWLIPLLIFLMLLLGLLLLLCWKYCACCKACLAMLPCCGPGRTVGFKEDHYMLRQSLMSSDYLNTPLVRSGSLKGGDTVRWKIDNNVHRQPPAPGTLNPKELVPYGVSLRLARLFTENLSKPQSRECTQLRQEVEDSLNEVYKVIPGTHKVKQTAFRLQSNAGKRQDHTIAETILMAPREAEPEIVKVTEKHVTQEAFQDMKVAPGYYTVTSDRDAHGLVEFQEGVELVDVRVPLFIREEDDDEKQLLVEALDVPSGIARLGRKLVNITIIKEQAKSIVTFVKPAYTYSRQEQVARVPVSREIIQNGKTQVTYRSKDLTAREGKDYTFSEGELTFQPDDTQSEILVPILGRSEVDTLLGNKQVKQFLLELSNPKYGAKIGKYPQCTITIDDTADVIQKTMTVQSVQSPRGKVGAPINLNAQALSARKIRLNWMPPPGKPTGYKVRYWIQGDSESDATVVDTNVPSAELVNLYPYCDYEMRVCAYNAQEEGPFTDIVTCQTLEEAPSEPGRLAFNVISSTVTQLSWAEPAETNGEITAYEVTYGMVNEDNKPIGPTKKVLINDSKKRMVLIENLRESQPYRYTVRACNKAGWGPERDATMNLATQPTRPMSIPIIPDAPIIDAEAGEEYDSYLMYSTDVLRSPVGSKRPSVSDESGQRWKYVPLLGSEMDLRRVTWKLPVEVIPRLSVSSGLSTDNESLLDQPLTTSSLTRKGTMRATPEEQLINGRTEFSFSGGSLNRSMVSGYQQLSPHVQNESYRFGSSSSQHTSTTVISGQGSSGQNRLGLPPLVLGGGRGRTHSEDVRDALTSLDVAIQDTRLSPGIPDTPTRLVFSALGPTSLKVSWQEPQCEREVLGYKVHYQLLHGGELRTIDITNPRENSVVVEDLLPNHSYMFRVKAQSVEGWGPEREGVITIESQVDPQSPLSPVPGSPFTLSTPSAPGPLVFTALAPDSLQLSWVRPRKPNGTILGYMITCEMLHGGGEPRTIYVEGDKPETSLTVPFLNENLPYKFKVQAKTTQGFGPEREGIITIESQDGGNFSQFGTQQITRREVFNMPGEYTQSSITQSNITEPFFSDGMSVTTRRIEGSNTVTQQVTKEFVTRTMMSGGSLNRQVERQFYDA

Target ORF information:

RefSeq Version	XM_018097872.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis integrin subunit beta 4 (itgb4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018097872.1

ORF Insert Sequence:

ATGATGAACC CTCGTGTTTT AGGGGGTCTC CTCCTGCTTG TCCTGCTCTT GGGTCTCCCA 
GCTTCCCATT CTCAGAGCAA CAAATATAAC CCCTGCGTGG CCTCCAGAGC AACCAGTTGC 
TCGGCCTGTA TCCGGGCGTA CAAGGACTGT TCCTACTGTG CAGATGAGGG TTTCGCTAGT 
GTACGCTGTG ATCTAGCAGA GAATCTGAGA AGATATGGCT GCAGCGAGAG AGGGATTGTT 
TACATGAGAA GTGAAGTCTT GACCCAGCAG AGCTATGACA TTAACACTCT CGTCAGCAAA 
ACCCAAGTGG CCCCTCAAAG GATGTCCATG CGTCTTCGTG CTGGGGAAGA AACCTCATTT 
GACCTGAATG TGTTTGAGCC GCTGGACACC CCTGTGGATT TGTACATTTT GATGGACTTC 
TCTCATTCCA TGTCAGATGA TTTAGCCAAC CTAAAAAATA TGGGTCAGGA GTTAGCTAAT 
GTTGTAAGCA CTCTCTCCAA GAATTACACC ATTGGATTTG GAAAATTTGT GGACAAAGTG 
ACTGTACCAC AGACTGACAT GAGACCTGAG AAACTGAAGC AACCCTGGTT GGACAGCACT 
CCTCCTTTCT CCTTCAAGAA CGTTATTCAA CTGACAAGCG ACGTGGGGAA ATTTCGGAAT 
GAATTGATGA AAGAGAAAAT CTCTGGAAAC CTTGATCCTC CAGAGGGGGG GTTTGATGCC 
ATTCTTCAGA CTGCTGTGTG CACGAATCAA ATTGGCTGGA GAAAAGGAAG TACTCATCTG 
TTGGTCTTCT CTACTGAGTC AGCATTCCAT TATGAAGCTG ATGGGATGAA CGTACTTGAT 
GGGATACTGA AGCGAAATGA TGAGCAGTGC CACTTGGATG CCTCTGGTTA CTACACACAC 
GACATCGTCC AGGACTATCC ATCTGTGCCC ACACTTGTTC GACTGTTAGC AAAGAACAAC 
ATCATCCCTA TCTTTGCCGT CACCAACTAC TCCTACAGTT ACTATGATAA GCTGAGCAAG 
TATTTCAAAG TATCTGAGAT TGGAGAATTG AAAGAAGATT CCTCCAACAT TGTGGAGCTC 
TTGCAGGAGG CCTTTACGCA AATTCGTTCC AGGATGGATA TCCGAGATGA TAACACCCCA 
CGTGCAATGG ATGTCTCAGT CACTTCTGAG ACAGCTAAAG TTACAGAAAC AGGATCGTTT 
CAAGTCACCC GGGGAGAAGT GGGTACCTTC AAAGTGAAGG TCAAGGCTTT AGAACGGTTG 
GGCAACCAGC ATGTGTGTGA ACTACCACCA CAAGACCAAG GGAGCCAAAT TCTCCTCAAA 
CCTTCCACCT TTACTGATGG CCTTAAGATT GATGCTTCTC TGATCTGTGA CAGCTGTCCT 
TGTGAATTGC AAAAGGAGTT GAGTTCCCCC AAGTGCAGCT TTAATGGCGA CTTTGTGTGC 
GGACGCTGTG AGTGCAGGGA GCGCTGGAGG GGAGTATATT GTAACTGCTC CAGCACAGAC 
ACCAAAAGCA CCCAATCGTG TATATCACCA GGCAGTACAG AGATTTGCTC CGGCAGGGGT 
GAATGCCTAT GCGGCACCTG TCAGTGTCAC TCGGAGTCCC CCACTCAACA GTACCAGGGG 
GAGTTCTGCC AGTTCAATAA CTTCCAGTGC CCACGTTTTC TTGGATACAT GTGCAATGAC 
AGAGGGCGCT GTCACATGGG AGCCTGTGTG TGTGATGCTG GCTGGGAAGG AGAGAGTTGT 
CAGTGTCCTA CCAGCAACCA AACCTGCCTT GATAGGAATG GGGGTCTGTG CAGTAGCCGT 
GGGAAGTGCG TGTGTGGGCG CTGCCAGTGT GATAACGTGT CACAGTATAC AGACGCCACG 
TGTGAATTCT CCTATTCACT GCGCCAACTG GGTGTGTGCG AAGACCTGCG GACGTGTGTG 
CAGTGCCAGG CCTGGGGAAC TGGAGAGAAA AAGGGCACAC AATGTGAAGC TTGTGAAATT 
AAGATTAAAA TGGTGGATGA GCTGAAGAAA GTGGATGAAA GAAGTGAAAA CTGTACTTTC 
CGGGATGAAA CAGATGACTG TACATATGAG TACTCAGTAG ACCCTCACGC AGTGCAAGGA 
AACTACACTG TGCTGGTCAA GAACAAAAAA GAATGTCCTC CTGGAGCCTT CCTCTGGCTT 
ATCCCTCTAC TCATCTTTCT GATGCTTCTC CTTGGGCTCT TGTTGCTCCT GTGCTGGAAA 
TACTGTGCCT GTTGCAAGGC TTGCTTGGCA ATGCTTCCCT GCTGTGGACC AGGACGGACA 
GTGGGATTTA AAGAAGACCA CTACATGTTA AGGCAAAGCC TGATGTCATC TGATTACCTG 
AATACCCCAC TGGTGCGTTC TGGGAGCCTC AAGGGCGGGG ACACTGTAAG ATGGAAGATC 
GACAACAACG TGCACCGCCA ACCACCCGCC CCTGGAACAC TGAACCCCAA GGAGCTTGTC 
CCATATGGCG TCTCACTGCG TCTGGCCCGG CTGTTCACTG AGAACCTGTC CAAGCCTCAA 
TCCCGTGAGT GTACCCAGCT GCGTCAGGAG GTGGAGGATT CGTTAAATGA GGTGTACAAG 
GTGATACCTG GAACCCACAA GGTGAAACAG ACAGCTTTCA GGCTTCAGTC CAATGCAGGA 
AAAAGACAGG ATCACACTAT TGCAGAAACC ATCCTGATGG CTCCCCGGGA GGCTGAACCT 
GAGATTGTAA AGGTTACAGA GAAACATGTA ACACAAGAGG CTTTCCAGGA TATGAAGGTT 
GCCCCAGGTT ACTATACAGT GACGTCAGAC AGAGATGCCC ATGGACTTGT AGAGTTCCAG 
GAGGGAGTTG AATTGGTGGA TGTCCGGGTT CCTCTCTTCA TCAGGGAAGA GGATGATGAT 
GAGAAGCAGT TGTTAGTGGA AGCTCTGGAC GTTCCATCAG GAATTGCCCG TTTGGGGCGG 
AAACTGGTCA ATATTACTAT TATCAAAGAG CAAGCCAAGA GCATAGTGAC GTTTGTGAAA 
CCTGCCTACA CCTACAGCCG ACAGGAACAG GTTGCCCGTG TCCCAGTTTC TCGTGAGATC 
ATCCAGAATG GAAAGACACA AGTTACATAT CGTTCCAAGG ACCTGACTGC TAGGGAGGGA 
AAGGACTATA CATTCTCAGA GGGGGAGCTC ACCTTCCAGC CAGATGACAC TCAATCAGAG 
ATTCTAGTGC CTATACTCGG TCGTTCAGAA GTTGACACTC TGCTGGGAAA TAAACAAGTC 
AAACAGTTCC TTCTAGAACT CAGTAATCCT AAGTATGGGG CCAAGATTGG CAAATACCCA 
CAATGCACCA TCACTATTGA TGACACTGCA GATGTTATAC AAAAGACAAT GACAGTCCAG 
TCTGTTCAGT CCCCCCGTGG GAAGGTAGGT GCTCCTATCA ACCTGAATGC CCAGGCCCTC 
AGCGCCAGGA AAATCCGACT CAACTGGATG CCGCCTCCTG GAAAACCCAC AGGTTACAAG 
GTGCGTTACT GGATTCAAGG AGATTCCGAG TCAGATGCGA CTGTGGTGGA TACAAATGTG 
CCCTCTGCAG AGCTGGTCAA TCTGTACCCC TACTGTGACT ACGAGATGCG GGTATGCGCA 
TACAATGCTC AAGAGGAGGG TCCCTTCACT GACATAGTCA CCTGCCAGAC CTTGGAGGAA 
GCACCCAGTG AACCCGGCCG CCTCGCCTTT AACGTCATTT CAAGCACAGT GACGCAGCTG 
AGTTGGGCAG AGCCAGCTGA AACCAATGGT GAAATCACAG CGTATGAGGT CACCTACGGA 
ATGGTCAACG AGGACAACAA ACCCATTGGG CCCACGAAGA AAGTCCTGAT TAATGATTCA 
AAGAAGCGCA TGGTTCTAAT TGAAAACCTA CGAGAATCCC AGCCATACCG ATACACTGTG 
AGAGCCTGCA ACAAAGCTGG GTGGGGGCCA GAGAGAGATG CCACCATGAA CCTAGCTACC 
CAACCCACCC GCCCAATGTC TATTCCCATT ATCCCTGATG CTCCCATTAT TGATGCTGAA 
GCTGGGGAAG AATATGACAG TTACCTGATG TACAGCACCG ATGTCCTGCG CTCTCCAGTT 
GGCAGCAAGA GACCCAGTGT GTCCGACGAG TCAGGCCAGC GCTGGAAGTA TGTCCCTCTC 
CTGGGCTCGG AGATGGACCT GCGGCGGGTC ACCTGGAAGC TACCCGTAGA GGTCATACCA 
CGCCTGTCCG TCAGCAGCGG CCTGTCCACT GACAACGAGA GCTTGCTGGA CCAGCCTTTG 
ACTACCAGCT CACTGACGAG GAAAGGGACC ATGCGAGCAA CCCCAGAAGA GCAATTAATT 
AACGGACGCA CAGAATTCTC CTTCTCGGGC GGCAGTCTCA ACCGTTCCAT GGTATCAGGG 
TACCAGCAAC TCAGCCCGCA CGTGCAAAAT GAAAGCTACA GGTTTGGTAG CAGCAGTTCC 
CAGCATACAT CCACAACGGT CATATCAGGG CAAGGCTCAT CAGGGCAGAA CAGACTGGGG 
CTCCCCCCAT TGGTGTTAGG AGGAGGAAGG GGACGAACCC ACTCAGAGGA TGTGAGGGAT 
GCTCTGACAA GCTTGGATGT GGCCATACAG GATACACGTC TCTCCCCGGG AATCCCGGAT 
ACCCCTACCC GTCTGGTATT CTCAGCTCTG GGACCCACCT CCCTAAAGGT CAGTTGGCAG 
GAGCCGCAAT GCGAAAGAGA AGTTCTGGGA TACAAAGTGC ATTATCAACT ACTGCATGGA 
GGTGAATTAA GGACCATCGA CATTACAAAC CCACGGGAGA ACTCAGTAGT GGTGGAAGAC 
TTGCTTCCTA ACCACTCGTA CATGTTCCGT GTGAAGGCAC AAAGCGTTGA GGGCTGGGGG 
CCCGAAAGAG AAGGCGTTAT CACTATCGAA TCACAGGTGG ATCCTCAGAG TCCACTCAGC 
CCTGTTCCAG GGTCTCCATT CACCCTAAGC ACCCCCAGTG CTCCTGGACC ACTGGTTTTC 
ACAGCTCTGG CCCCTGACTC CCTGCAGTTA AGTTGGGTGA GACCCCGAAA GCCTAATGGA 
ACAATTCTGG GGTACATGAT CACTTGCGAG ATGTTGCATG GAGGAGGGGA ACCACGAACG 
ATCTATGTTG AAGGTGACAA ACCCGAGACT TCCCTGACGG TTCCTTTCCT AAATGAAAAT 
CTGCCCTATA AATTTAAGGT CCAGGCGAAA ACCACACAGG GTTTTGGACC AGAACGGGAA 
GGGATAATTA CTATTGAATC TCAAGATGGA GGAAACTTTT CTCAGTTTGG AACACAACAG 
ATCACACGCC GAGAGGTGTT CAATATGCCA GGAGAATATA CTCAAAGCAG CATCACTCAA 
TCCAACATAA CAGAACCCTT CTTTTCAGAT GGAATGAGTG TCACCACCCG CCGTATCGAG 
GGCTCCAACA CAGTCACACA ACAGGTCACC AAAGAATTTG TCACACGGAC CATGATGTCT 
GGCGGCTCCC TCAACAGGCA AGTGGAGCGC CAGTTTTACG ACGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itgb4 ( XM_004918457.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004918514.1 Xenopus tropicalis integrin subunit beta 4 (itgb4), transcript variant X3, mRNA.

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CloneID	OXa17191
Clone ID Related Accession (Same CDS sequence)	XM_004918457.3
Accession Version	XM_004918457.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	integrin beta-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004918457.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAACC CTCGTGTTTT AGGGGGTCTC CTCCTGCTTG TCCTGCTCTT GGGTCTCCCA 
GCTTCCCATT CTCAGAGCAA CAAATATAAC CCCTGCGTGG CCTCCAGAGC AACCAGTTGC 
TCGGCCTGTA TCCGGGCGTA CAAGGACTGT TCCTACTGTG CAGATGAGGG TTTCGCTAGT 
GTACGCTGTG ATCTAGCAGA GAATCTGAGA AGATATGGCT GCAGCGAGAG AGGGATTGTT 
TACATGAGAA GTGAAGTCTT GACCCAGCAG AGCTATGACA TTAACACTCT CGTCAGCAAA 
ACCCAAGTGG CCCCTCAAAG GATGTCCATG CGTCTTCGTG CTGGGGAAGA AACCTCATTT 
GACCTGAATG TGTTTGAGCC GCTGGACACC CCTGTGGATT TGTACATTTT GATGGACTTC 
TCTCATTCCA TGTCAGATGA TTTAGCCAAC CTAAAAAATA TGGGTCAGGA GTTAGCTAAT 
GTTGTAAGCA CTCTCTCCAA GAATTACACC ATTGGATTTG GAAAATTTGT GGACAAAGTG 
ACTGTACCAC AGACTGACAT GAGACCTGAG AAACTGAAGC AACCCTGGTT GGACAGCACT 
CCTCCTTTCT CCTTCAAGAA CGTTATTCAA CTGACAAGCG ACGTGGGGAA ATTTCGGAAT 
GAATTGATGA AAGAGAAAAT CTCTGGAAAC CTTGATCCTC CAGAGGGGGG GTTTGATGCC 
ATTCTTCAGA CTGCTGTGTG CACGAATCAA ATTGGCTGGA GAAAAGGAAG TACTCATCTG 
TTGGTCTTCT CTACTGAGTC AGCATTCCAT TATGAAGCTG ATGGGATGAA CGTACTTGAT 
GGGATACTGA AGCGAAATGA TGAGCAGTGC CACTTGGATG CCTCTGGTTA CTACACACAC 
GACATCGTCC AGGACTATCC ATCTGTGCCC ACACTTGTTC GACTGTTAGC AAAGAACAAC 
ATCATCCCTA TCTTTGCCGT CACCAACTAC TCCTACAGTT ACTATGATAA GCTGAGCAAG 
TATTTCAAAG TATCTGAGAT TGGAGAATTG AAAGAAGATT CCTCCAACAT TGTGGAGCTC 
TTGCAGGAGG CCTTTACGCA AATTCGTTCC AGGATGGATA TCCGAGATGA TAACACCCCA 
CGTGCAATGG ATGTCTCAGT CACTTCTGAG ACAGCTAAAG TTACAGAAAC AGGATCGTTT 
CAAGTCACCC GGGGAGAAGT GGGTACCTTC AAAGTGAAGG TCAAGGCTTT AGAACGGTTG 
GGCAACCAGC ATGTGTGTGA ACTACCACCA CAAGACCAAG GGAGCCAAAT TCTCCTCAAA 
CCTTCCACCT TTACTGATGG CCTTAAGATT GATGCTTCTC TGATCTGTGA CAGCTGTCCT 
TGTGAATTGC AAAAGGAGTT GAGTTCCCCC AAGTGCAGCT TTAATGGCGA CTTTGTGTGC 
GGACGCTGTG AGTGCAGGGA GCGCTGGAGG GGAGTATATT GTAACTGCTC CAGCACAGAC 
ACCAAAAGCA CCCAATCGTG TATATCACCA GGCAGTACAG AGATTTGCTC CGGCAGGGGT 
GAATGCCTAT GCGGCACCTG TCAGTGTCAC TCGGAGTCCC CCACTCAACA GTACCAGGGG 
GAGTTCTGCC AGTTCAATAA CTTCCAGTGC CCACGTTTTC TTGGATACAT GTGCAATGAC 
AGAGGGCGCT GTCACATGGG AGCCTGTGTG TGTGATGCTG GCTGGGAAGG AGAGAGTTGT 
CAGTGTCCTA CCAGCAACCA AACCTGCCTT GATAGGAATG GGGGTCTGTG CAGTAGCCGT 
GGGAAGTGCG TGTGTGGGCG CTGCCAGTGT GATAACGTGT CACAGTATAC AGACGCCACG 
TGTGAATTCT CCTATTCACT GCGCCAACTG GGTGTGTGCG AAGACCTGCG GACGTGTGTG 
CAGTGCCAGG CCTGGGGAAC TGGAGAGAAA AAGGGCACAC AATGTGAAGC TTGTGAAATT 
AAGATTAAAA TGGTGGATGA GCTGAAGAAA GTGGATGAAA GAAGTGAAAA CTGTACTTTC 
CGGGATGAAA CAGATGACTG TACATATGAG TACTCAGTAG ACCCTCACGC AGTGCAAGGA 
AACTACACTG TGCTGGTCAA GAACAAAAAA GAATGTCCTC CTGGAGCCTT CCTCTGGCTT 
ATCCCTCTAC TCATCTTTCT GATGCTTCTC CTTGGGCTCT TGTTGCTCCT GTGCTGGAAA 
TACTGTGCCT GTTGCAAGGC TTGCTTGGCA ATGCTTCCCT GCTGTGGACC AGGACGGACA 
GTGGGATTTA AAGAAGACCA CTACATGTTA AGGCAAAGCC TGATGTCATC TGATTACCTG 
AATACCCCAC TGGTGCGTTC TGGGAGCCTC AAGGGCGGGG ACACTGTAAG ATGGAAGATC 
GACAACAACG TGCACCGCCA ACCACCCGCC CCTGGAACAC TGAACCCCAA GGAGCTTGTC 
CCATATGGCG TCTCACTGCG TCTGGCCCGG CTGTTCACTG AGAACCTGTC CAAGCCTCAA 
TCCCGTGAGT GTACCCAGCT GCGTCAGGAG GTGGAGGATT CGTTAAATGA GGTGTACAAG 
GTGATACCTG GAACCCACAA GGTGAAACAG ACAGCTTTCA GGCTTCAGTC CAATGCAGGA 
AAAAGACAGG ATCACACTAT TGCAGAAACC ATCCTGATGG CTCCCCGGGA GGCTGAACCT 
GAGATTGTAA AGGTTACAGA GAAACATGTA ACACAAGAGG CTTTCCAGGA TATGAAGGTT 
GCCCCAGGTT ACTATACAGT GACGTCAGAC AGAGATGCCC ATGGACTTGT AGAGTTCCAG 
GAGGGAGTTG AATTGGTGGA TGTCCGGGTT CCTCTCTTCA TCAGGGAAGA GGATGATGAT 
GAGAAGCAGT TGTTAGTGGA AGCTCTGGAC GTTCCATCAG GAATTGCCCG TTTGGGGCGG 
AAACTGGTCA ATATTACTAT TATCAAAGAG CAAGCCAAGA GCATAGTGAC GTTTGTGAAA 
CCTGCCTACA CCTACAGCCG ACAGGAACAG GTTGCCCGTG TCCCAGTTTC TCGTGAGATC 
ATCCAGAATG GAAAGACACA AGTTACATAT CGTTCCAAGG ACCTGACTGC TAGGGAGGGA 
AAGGACTATA CATTCTCAGA GGGGGAGCTC ACCTTCCAGC CAGATGACAC TCAATCAGAG 
ATTCTAGTGC CTATACTCGG TCGTTCAGAA GTTGACACTC TGCTGGGAAA TAAACAAGTC 
AAACAGTTCC TTCTAGAACT CAGTAATCCT AAGTATGGGG CCAAGATTGG CAAATACCCA 
CAATGCACCA TCACTATTGA TGACACTGCA GATGTTATAC AAAAGACAAT GACAGTCCAG 
TCTGTTCAGT CCCCCCGTGG GAAGGTAGGT GCTCCTATCA ACCTGAATGC CCAGGCCCTC 
AGCGCCAGGA AAATCCGACT CAACTGGATG CCGCCTCCTG GAAAACCCAC AGGTTACAAG 
GTGCGTTACT GGATTCAAGG AGATTCCGAG TCAGATGCGA CTGTGGTGGA TACAAATGTG 
CCCTCTGCAG AGCTGGTCAA TCTGTACCCC TACTGTGACT ACGAGATGCG GGTATGCGCA 
TACAATGCTC AAGAGGAGGG TCCCTTCACT GACATAGTCA CCTGCCAGAC CTTGGAGGAA 
GCACCCAGTG AACCCGGCCG CCTCGCCTTT AACGTCATTT CAAGCACAGT GACGCAGCTG 
AGTTGGGCAG AGCCAGCTGA AACCAATGGT GAAATCACAG CGTATGAGGT CACCTACGGA 
ATGGTCAACG AGGACAACAA ACCCATTGGG CCCACGAAGA AAGTCCTGAT TAATGATTCA 
AAGAAGCGCA TGGTTCTAAT TGAAAACCTA CGAGAATCCC AGCCATACCG ATACACTGTG 
AGAGCCTGCA ACAAAGCTGG GTGGGGGCCA GAGAGAGATG CCACCATGAA CCTAGCTACC 
CAACCCACCC GCCCAATGTC TATTCCCATT ATCCCTGATG CTCCCATTAT TGATGCTGAA 
GCTGGGGAAG AATATGACAG TTACCTGATG TACAGCACCG ATGTCCTGCG CTCTCCAGTT 
GGCAGCAAGA GACCCAGTGT GTCCGACGAG TCAGAGCAAT TAATTAACGG ACGCACAGAA 
TTCTCCTTCT CGGGCGGCAG TCTCAACCGT TCCATGGTAT CAGGGTACCA GCAACTCAGC 
CCGCACGTGC AAAATGAAAG CTACAGGTTT GGTAGCAGCA GTTCCCAGCA TACATCCACA 
ACGGTCATAT CAGGGCAAGG CTCATCAGGG CAGAACAGAC TGGGGCTCCC CCCATTGGTG 
TTAGGAGGAG GAAGGGGACG AACCCACTCA GAGGATGTGA GGGATGCTCT GACAAGCTTG 
GATGTGGCCA TACAGGATAC ACGTCTCTCC CCGGGAATCC CGGATACCCC TACCCGTCTG 
GTATTCTCAG CTCTGGGACC CACCTCCCTA AAGGTCAGTT GGCAGGAGCC GCAATGCGAA 
AGAGAAGTTC TGGGATACAA AGTGCATTAT CAACTACTGC ATGGAGGTGA ATTAAGGACC 
ATCGACATTA CAAACCCACG GGAGAACTCA GTAGTGGTGG AAGACTTGCT TCCTAACCAC 
TCGTACATGT TCCGTGTGAA GGCACAAAGC GTTGAGGGCT GGGGGCCCGA AAGAGAAGGC 
GTTATCACTA TCGAATCACA GGTGGATCCT CAGAGTCCAC TCAGCCCTGT TCCAGGGTCT 
CCATTCACCC TAAGCACCCC CAGTGCTCCT GGACCACTGG TTTTCACAGC TCTGGCCCCT 
GACTCCCTGC AGTTAAGTTG GGTGAGACCC CGAAAGCCTA ATGGAACAAT TCTGGGGTAC 
ATGATCACTT GCGAGATGTT GCATGGAGGA GGGGAACCAC GAACGATCTA TGTTGAAGGT 
GACAAACCCG AGACTTCCCT GACGGTTCCT TTCCTAAATG AAAATCTGCC CTATAAATTT 
AAGGTCCAGG CGAAAACCAC ACAGGGTTTT GGACCAGAAC GGGAAGGGAT AATTACTATT 
GAATCTCAAG ATGGAGGAAA CTTTTCTCAG TTTGGAACAC AACAGATCAC ACGCCGAGAG 
GTGTTCAATA TGCCAGGAGA ATATACTCAA AGCAGCATCA CTCAATCCAA CATAACAGAA 
CCCTTCTTTT CAGATGGAAT GAGTGTCACC ACCCGCCGTA TCGAGGGCTC CAACACAGTC 
ACACAACAGG TCACCAAAGA ATTTGTCACA CGGACCATGA TGTCTGGCGG CTCCCTCAAC 
AGGCAAGTGG AGCGCCAGTT TTACGACGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004918514.1
CDS	1125..6437
Translation	MMNPRVLGGLLLLVLLLGLPASHSQSNKYNPCVASRATSCSACIRAYKDCSYCADEGFASVRCDLAENLRRYGCSERGIVYMRSEVLTQQSYDINTLVSKTQVAPQRMSMRLRAGEETSFDLNVFEPLDTPVDLYILMDFSHSMSDDLANLKNMGQELANVVSTLSKNYTIGFGKFVDKVTVPQTDMRPEKLKQPWLDSTPPFSFKNVIQLTSDVGKFRNELMKEKISGNLDPPEGGFDAILQTAVCTNQIGWRKGSTHLLVFSTESAFHYEADGMNVLDGILKRNDEQCHLDASGYYTHDIVQDYPSVPTLVRLLAKNNIIPIFAVTNYSYSYYDKLSKYFKVSEIGELKEDSSNIVELLQEAFTQIRSRMDIRDDNTPRAMDVSVTSETAKVTETGSFQVTRGEVGTFKVKVKALERLGNQHVCELPPQDQGSQILLKPSTFTDGLKIDASLICDSCPCELQKELSSPKCSFNGDFVCGRCECRERWRGVYCNCSSTDTKSTQSCISPGSTEICSGRGECLCGTCQCHSESPTQQYQGEFCQFNNFQCPRFLGYMCNDRGRCHMGACVCDAGWEGESCQCPTSNQTCLDRNGGLCSSRGKCVCGRCQCDNVSQYTDATCEFSYSLRQLGVCEDLRTCVQCQAWGTGEKKGTQCEACEIKIKMVDELKKVDERSENCTFRDETDDCTYEYSVDPHAVQGNYTVLVKNKKECPPGAFLWLIPLLIFLMLLLGLLLLLCWKYCACCKACLAMLPCCGPGRTVGFKEDHYMLRQSLMSSDYLNTPLVRSGSLKGGDTVRWKIDNNVHRQPPAPGTLNPKELVPYGVSLRLARLFTENLSKPQSRECTQLRQEVEDSLNEVYKVIPGTHKVKQTAFRLQSNAGKRQDHTIAETILMAPREAEPEIVKVTEKHVTQEAFQDMKVAPGYYTVTSDRDAHGLVEFQEGVELVDVRVPLFIREEDDDEKQLLVEALDVPSGIARLGRKLVNITIIKEQAKSIVTFVKPAYTYSRQEQVARVPVSREIIQNGKTQVTYRSKDLTAREGKDYTFSEGELTFQPDDTQSEILVPILGRSEVDTLLGNKQVKQFLLELSNPKYGAKIGKYPQCTITIDDTADVIQKTMTVQSVQSPRGKVGAPINLNAQALSARKIRLNWMPPPGKPTGYKVRYWIQGDSESDATVVDTNVPSAELVNLYPYCDYEMRVCAYNAQEEGPFTDIVTCQTLEEAPSEPGRLAFNVISSTVTQLSWAEPAETNGEITAYEVTYGMVNEDNKPIGPTKKVLINDSKKRMVLIENLRESQPYRYTVRACNKAGWGPERDATMNLATQPTRPMSIPIIPDAPIIDAEAGEEYDSYLMYSTDVLRSPVGSKRPSVSDESEQLINGRTEFSFSGGSLNRSMVSGYQQLSPHVQNESYRFGSSSSQHTSTTVISGQGSSGQNRLGLPPLVLGGGRGRTHSEDVRDALTSLDVAIQDTRLSPGIPDTPTRLVFSALGPTSLKVSWQEPQCEREVLGYKVHYQLLHGGELRTIDITNPRENSVVVEDLLPNHSYMFRVKAQSVEGWGPEREGVITIESQVDPQSPLSPVPGSPFTLSTPSAPGPLVFTALAPDSLQLSWVRPRKPNGTILGYMITCEMLHGGGEPRTIYVEGDKPETSLTVPFLNENLPYKFKVQAKTTQGFGPEREGIITIESQDGGNFSQFGTQQITRREVFNMPGEYTQSSITQSNITEPFFSDGMSVTTRRIEGSNTVTQQVTKEFVTRTMMSGGSLNRQVERQFYDA

Target ORF information:

RefSeq Version	XM_004918457.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis integrin subunit beta 4 (itgb4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004918457.3

ORF Insert Sequence:

ATGATGAACC CTCGTGTTTT AGGGGGTCTC CTCCTGCTTG TCCTGCTCTT GGGTCTCCCA 
GCTTCCCATT CTCAGAGCAA CAAATATAAC CCCTGCGTGG CCTCCAGAGC AACCAGTTGC 
TCGGCCTGTA TCCGGGCGTA CAAGGACTGT TCCTACTGTG CAGATGAGGG TTTCGCTAGT 
GTACGCTGTG ATCTAGCAGA GAATCTGAGA AGATATGGCT GCAGCGAGAG AGGGATTGTT 
TACATGAGAA GTGAAGTCTT GACCCAGCAG AGCTATGACA TTAACACTCT CGTCAGCAAA 
ACCCAAGTGG CCCCTCAAAG GATGTCCATG CGTCTTCGTG CTGGGGAAGA AACCTCATTT 
GACCTGAATG TGTTTGAGCC GCTGGACACC CCTGTGGATT TGTACATTTT GATGGACTTC 
TCTCATTCCA TGTCAGATGA TTTAGCCAAC CTAAAAAATA TGGGTCAGGA GTTAGCTAAT 
GTTGTAAGCA CTCTCTCCAA GAATTACACC ATTGGATTTG GAAAATTTGT GGACAAAGTG 
ACTGTACCAC AGACTGACAT GAGACCTGAG AAACTGAAGC AACCCTGGTT GGACAGCACT 
CCTCCTTTCT CCTTCAAGAA CGTTATTCAA CTGACAAGCG ACGTGGGGAA ATTTCGGAAT 
GAATTGATGA AAGAGAAAAT CTCTGGAAAC CTTGATCCTC CAGAGGGGGG GTTTGATGCC 
ATTCTTCAGA CTGCTGTGTG CACGAATCAA ATTGGCTGGA GAAAAGGAAG TACTCATCTG 
TTGGTCTTCT CTACTGAGTC AGCATTCCAT TATGAAGCTG ATGGGATGAA CGTACTTGAT 
GGGATACTGA AGCGAAATGA TGAGCAGTGC CACTTGGATG CCTCTGGTTA CTACACACAC 
GACATCGTCC AGGACTATCC ATCTGTGCCC ACACTTGTTC GACTGTTAGC AAAGAACAAC 
ATCATCCCTA TCTTTGCCGT CACCAACTAC TCCTACAGTT ACTATGATAA GCTGAGCAAG 
TATTTCAAAG TATCTGAGAT TGGAGAATTG AAAGAAGATT CCTCCAACAT TGTGGAGCTC 
TTGCAGGAGG CCTTTACGCA AATTCGTTCC AGGATGGATA TCCGAGATGA TAACACCCCA 
CGTGCAATGG ATGTCTCAGT CACTTCTGAG ACAGCTAAAG TTACAGAAAC AGGATCGTTT 
CAAGTCACCC GGGGAGAAGT GGGTACCTTC AAAGTGAAGG TCAAGGCTTT AGAACGGTTG 
GGCAACCAGC ATGTGTGTGA ACTACCACCA CAAGACCAAG GGAGCCAAAT TCTCCTCAAA 
CCTTCCACCT TTACTGATGG CCTTAAGATT GATGCTTCTC TGATCTGTGA CAGCTGTCCT 
TGTGAATTGC AAAAGGAGTT GAGTTCCCCC AAGTGCAGCT TTAATGGCGA CTTTGTGTGC 
GGACGCTGTG AGTGCAGGGA GCGCTGGAGG GGAGTATATT GTAACTGCTC CAGCACAGAC 
ACCAAAAGCA CCCAATCGTG TATATCACCA GGCAGTACAG AGATTTGCTC CGGCAGGGGT 
GAATGCCTAT GCGGCACCTG TCAGTGTCAC TCGGAGTCCC CCACTCAACA GTACCAGGGG 
GAGTTCTGCC AGTTCAATAA CTTCCAGTGC CCACGTTTTC TTGGATACAT GTGCAATGAC 
AGAGGGCGCT GTCACATGGG AGCCTGTGTG TGTGATGCTG GCTGGGAAGG AGAGAGTTGT 
CAGTGTCCTA CCAGCAACCA AACCTGCCTT GATAGGAATG GGGGTCTGTG CAGTAGCCGT 
GGGAAGTGCG TGTGTGGGCG CTGCCAGTGT GATAACGTGT CACAGTATAC AGACGCCACG 
TGTGAATTCT CCTATTCACT GCGCCAACTG GGTGTGTGCG AAGACCTGCG GACGTGTGTG 
CAGTGCCAGG CCTGGGGAAC TGGAGAGAAA AAGGGCACAC AATGTGAAGC TTGTGAAATT 
AAGATTAAAA TGGTGGATGA GCTGAAGAAA GTGGATGAAA GAAGTGAAAA CTGTACTTTC 
CGGGATGAAA CAGATGACTG TACATATGAG TACTCAGTAG ACCCTCACGC AGTGCAAGGA 
AACTACACTG TGCTGGTCAA GAACAAAAAA GAATGTCCTC CTGGAGCCTT CCTCTGGCTT 
ATCCCTCTAC TCATCTTTCT GATGCTTCTC CTTGGGCTCT TGTTGCTCCT GTGCTGGAAA 
TACTGTGCCT GTTGCAAGGC TTGCTTGGCA ATGCTTCCCT GCTGTGGACC AGGACGGACA 
GTGGGATTTA AAGAAGACCA CTACATGTTA AGGCAAAGCC TGATGTCATC TGATTACCTG 
AATACCCCAC TGGTGCGTTC TGGGAGCCTC AAGGGCGGGG ACACTGTAAG ATGGAAGATC 
GACAACAACG TGCACCGCCA ACCACCCGCC CCTGGAACAC TGAACCCCAA GGAGCTTGTC 
CCATATGGCG TCTCACTGCG TCTGGCCCGG CTGTTCACTG AGAACCTGTC CAAGCCTCAA 
TCCCGTGAGT GTACCCAGCT GCGTCAGGAG GTGGAGGATT CGTTAAATGA GGTGTACAAG 
GTGATACCTG GAACCCACAA GGTGAAACAG ACAGCTTTCA GGCTTCAGTC CAATGCAGGA 
AAAAGACAGG ATCACACTAT TGCAGAAACC ATCCTGATGG CTCCCCGGGA GGCTGAACCT 
GAGATTGTAA AGGTTACAGA GAAACATGTA ACACAAGAGG CTTTCCAGGA TATGAAGGTT 
GCCCCAGGTT ACTATACAGT GACGTCAGAC AGAGATGCCC ATGGACTTGT AGAGTTCCAG 
GAGGGAGTTG AATTGGTGGA TGTCCGGGTT CCTCTCTTCA TCAGGGAAGA GGATGATGAT 
GAGAAGCAGT TGTTAGTGGA AGCTCTGGAC GTTCCATCAG GAATTGCCCG TTTGGGGCGG 
AAACTGGTCA ATATTACTAT TATCAAAGAG CAAGCCAAGA GCATAGTGAC GTTTGTGAAA 
CCTGCCTACA CCTACAGCCG ACAGGAACAG GTTGCCCGTG TCCCAGTTTC TCGTGAGATC 
ATCCAGAATG GAAAGACACA AGTTACATAT CGTTCCAAGG ACCTGACTGC TAGGGAGGGA 
AAGGACTATA CATTCTCAGA GGGGGAGCTC ACCTTCCAGC CAGATGACAC TCAATCAGAG 
ATTCTAGTGC CTATACTCGG TCGTTCAGAA GTTGACACTC TGCTGGGAAA TAAACAAGTC 
AAACAGTTCC TTCTAGAACT CAGTAATCCT AAGTATGGGG CCAAGATTGG CAAATACCCA 
CAATGCACCA TCACTATTGA TGACACTGCA GATGTTATAC AAAAGACAAT GACAGTCCAG 
TCTGTTCAGT CCCCCCGTGG GAAGGTAGGT GCTCCTATCA ACCTGAATGC CCAGGCCCTC 
AGCGCCAGGA AAATCCGACT CAACTGGATG CCGCCTCCTG GAAAACCCAC AGGTTACAAG 
GTGCGTTACT GGATTCAAGG AGATTCCGAG TCAGATGCGA CTGTGGTGGA TACAAATGTG 
CCCTCTGCAG AGCTGGTCAA TCTGTACCCC TACTGTGACT ACGAGATGCG GGTATGCGCA 
TACAATGCTC AAGAGGAGGG TCCCTTCACT GACATAGTCA CCTGCCAGAC CTTGGAGGAA 
GCACCCAGTG AACCCGGCCG CCTCGCCTTT AACGTCATTT CAAGCACAGT GACGCAGCTG 
AGTTGGGCAG AGCCAGCTGA AACCAATGGT GAAATCACAG CGTATGAGGT CACCTACGGA 
ATGGTCAACG AGGACAACAA ACCCATTGGG CCCACGAAGA AAGTCCTGAT TAATGATTCA 
AAGAAGCGCA TGGTTCTAAT TGAAAACCTA CGAGAATCCC AGCCATACCG ATACACTGTG 
AGAGCCTGCA ACAAAGCTGG GTGGGGGCCA GAGAGAGATG CCACCATGAA CCTAGCTACC 
CAACCCACCC GCCCAATGTC TATTCCCATT ATCCCTGATG CTCCCATTAT TGATGCTGAA 
GCTGGGGAAG AATATGACAG TTACCTGATG TACAGCACCG ATGTCCTGCG CTCTCCAGTT 
GGCAGCAAGA GACCCAGTGT GTCCGACGAG TCAGAGCAAT TAATTAACGG ACGCACAGAA 
TTCTCCTTCT CGGGCGGCAG TCTCAACCGT TCCATGGTAT CAGGGTACCA GCAACTCAGC 
CCGCACGTGC AAAATGAAAG CTACAGGTTT GGTAGCAGCA GTTCCCAGCA TACATCCACA 
ACGGTCATAT CAGGGCAAGG CTCATCAGGG CAGAACAGAC TGGGGCTCCC CCCATTGGTG 
TTAGGAGGAG GAAGGGGACG AACCCACTCA GAGGATGTGA GGGATGCTCT GACAAGCTTG 
GATGTGGCCA TACAGGATAC ACGTCTCTCC CCGGGAATCC CGGATACCCC TACCCGTCTG 
GTATTCTCAG CTCTGGGACC CACCTCCCTA AAGGTCAGTT GGCAGGAGCC GCAATGCGAA 
AGAGAAGTTC TGGGATACAA AGTGCATTAT CAACTACTGC ATGGAGGTGA ATTAAGGACC 
ATCGACATTA CAAACCCACG GGAGAACTCA GTAGTGGTGG AAGACTTGCT TCCTAACCAC 
TCGTACATGT TCCGTGTGAA GGCACAAAGC GTTGAGGGCT GGGGGCCCGA AAGAGAAGGC 
GTTATCACTA TCGAATCACA GGTGGATCCT CAGAGTCCAC TCAGCCCTGT TCCAGGGTCT 
CCATTCACCC TAAGCACCCC CAGTGCTCCT GGACCACTGG TTTTCACAGC TCTGGCCCCT 
GACTCCCTGC AGTTAAGTTG GGTGAGACCC CGAAAGCCTA ATGGAACAAT TCTGGGGTAC 
ATGATCACTT GCGAGATGTT GCATGGAGGA GGGGAACCAC GAACGATCTA TGTTGAAGGT 
GACAAACCCG AGACTTCCCT GACGGTTCCT TTCCTAAATG AAAATCTGCC CTATAAATTT 
AAGGTCCAGG CGAAAACCAC ACAGGGTTTT GGACCAGAAC GGGAAGGGAT AATTACTATT 
GAATCTCAAG ATGGAGGAAA CTTTTCTCAG TTTGGAACAC AACAGATCAC ACGCCGAGAG 
GTGTTCAATA TGCCAGGAGA ATATACTCAA AGCAGCATCA CTCAATCCAA CATAACAGAA 
CCCTTCTTTT CAGATGGAAT GAGTGTCACC ACCCGCCGTA TCGAGGGCTC CAACACAGTC 
ACACAACAGG TCACCAAAGA ATTTGTCACA CGGACCATGA TGTCTGGCGG CTCCCTCAAC 
AGGCAAGTGG AGCGCCAGTT TTACGACGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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itgb4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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