Ppl cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppl
Entrez Gene ID	19041
Full Name	periplakin
Synonyms	AW553870
General protein information	Preferred Names periplakin Names periplakin 195kDa protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16 A1\|16 2.5 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008909.2	NP_032935.2	periplakin

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PPL	XP_003314985.2
Gallus gallus (chicken)	PPL	XP_414712.3
Homo sapiens (human)	PPL	NP_002696.3
	PPL	XP_001098863.2
Canis lupus familiaris (dog)	PPL	XP_005621642.1
Xenopus tropicalis (tropical clawed frog)	ppl	XP_002941094.2
Bos taurus (cattle)	PPL	XP_005224719.1
Mus musculus (house mouse)	Ppl	NP_032935.2
Rattus norvegicus (Norway rat)	Ppl	NP_001100446.1
Danio rerio (zebrafish)	ppl	NP_001131138.1

Gene Ontology
Bibliography

Related articles in PubMed

Increased Bacterial Load and Expression of Antimicrobial Peptides in Skin of Barrier-Deficient Mice with Reduced Cancer Susceptibility.
Natsuga K, Cipolat S, Watt FM
The Journal of investigative dermatology136(1)99-106(2016 Jan)

Epidermal barrier defects link atopic dermatitis with altered skin cancer susceptibility.
Cipolat S, Hoste E, Natsuga K, Quist SR, Watt FM
eLife3e01888(2014 May)

Annexin A9 is a periplakin interacting partner in membrane-targeted cytoskeletal linker protein complexes.
Boczonadi V, M??tt? A
FEBS letters586(19)3090-6(2012 Sep)

Exons 5-15 of kazrin are dispensable for murine epidermal morphogenesis and homeostasis.
Chhatriwala MK, Cipolat S, Sevilla LM, Nachat R, Watt FM
The Journal of investigative dermatology132(8)1977-87(2012 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Thus, combined loss of the cornified envelope proteins not only impairs the epidermal barrier, but also changes the composition of T cell subpopulations in the skin.
Title: Mice deficient in involucrin, envoplakin, and periplakin have a defective epidermal barrier.

Periplakin interferes with G protein activation by the melanin-concentrating hormone receptor-1 by binding to the proximal segment of the receptor C-terminal tail
Title: Periplakin interferes with G protein activation by the melanin-concentrating hormone receptor-1 by binding to the proximal segment of the receptor C-terminal tail.

Data suggest that the primary role of periplakin may not relate to the physiology of the cornified cell envelope in epidermal keratinocytes but may reside in the challenges, which normal laboratory mice do not encounter.
Title: Periplakin gene targeting reveals a constituent of the cornified cell envelope dispensable for normal mouse development.

The following Ppl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu03066	NM_008909.2 Latest version!	Mus musculus periplakin (Ppl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppl ( NM_008909.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032935.2 Mus musculus periplakin (Ppl), mRNA.

Price & Availability↑

CloneID	OMu03066
Clone ID Related Accession (Same CDS sequence)	NM_008909.2
Accession Version	NM_008909.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-07
Organism	Mus musculus(house mouse)
Product	periplakin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ056283.1, AK142562.1, AF126834.1, AC163723.3 and AW553870.2. On Aug 17, 2006 this sequence version replaced NM_008909.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF126834.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCAACGGT GCAGACGCGG 
AGCATCTCCA ACAAGGAACT CTCAGATCTG ATTGAGCAGC TGCAGAAGAA TGCAGACCAG 
GTGGAAAGGA ACATTGTGGA CACCGAGGCC AAGATGCAGA GCGACTTGGC CCGGATGCAA 
GAGGGGCAGC TCCCTGAGCA CCGGGACGCG GCCCTGCAGA ACGTGTCTGA CTCAGAAAAG 
CTGCTGTACG TGCTGGAGGC AGATTCGGCC ATCGCCAAGC ACATGAAGCA CCCACAGGGG 
GACATGATCG CCGAGGACAT CCGCCAGCTG AAAGAGCGCG TGACCAACCT GCGGGGGAAA 
CACAAGCAGA TGTACAGCCT GGCAGTGAAA GAGGCCGACC CGAGAGTCAA CTGGGACACA 
CTGGTGGACG AGAAGCTGGA CAAGCTGAGC AGCCAGAGCT TTGGGACTGA CCTTCCTCTG 
GTGGATAGCC AGGTGGAGCA ACACAACATC TTCCACAATG AAGTCAAAGC CATCGGGCCC 
CACCTGGCCA AGGACAAGGA GCAGAACAGT GAACTCCAAG CCAAGTATCA GAAGTTGCTG 
ACAGCATCAC AGGCACGGCA GCAGCACCTG AGCTCGCTGC AGGATTACAT GCAGCGCTGC 
ACCAATGAGC TGTACTGGCT GGACCAGCAA GCCAAGGGCC GCATGCAGTA TGACTGGAGC 
GACCGCAACC TCGACTACCC CAGCCGCCGC CGCCAGTACG AGAATTTCAT CAACCGGAAC 
CTGGAGGCCA AAGAAGAAAG AATCAACAAA CTGCACACTG AAGGTGACCA ACTGCTGACA 
GCTGAGCACC CAGGGAGGAA CTCCATTGAG GCACACATGG AGGCTGTGCA TGCTGAGTGG 
AAGGAGTACC TGAACCTGCT CATCTGTGAG GAGAGCCATC TGAAATACAT GGAAGACTAC 
CACCAATTTC ACAAAGACAT GAAGGATGCT CAGGAGCTGC TACGCAAGGT GGACTCAGAC 
TTGAACCAGA AATATAGCCC TGACTTCAAA GACCGGTATC AGATAGAGCT GTTGCTTCGG 
GAACTGGATG ACCAGGAGAA GGCTCTGGAC AAGTATGAGG ATGTGGTGCG AGGGCTGCAG 
AGGCGAGGGC AGCAAGTGGT GCCACTCAAG TACCGCCGCG AGACGCCGCT CAAGCCCATC 
CCTGTGGAGG CTCTCTGTGA CTTTGAGAGT GACCAGGGCC TCATCTCGCG GGGCTATAGC 
TACACACTGC AGAAGAACAA CGGGGAGAGC TGGGAGCTGA CAGACAGCAC TGGGAAGAAG 
CTGGCTGCCC CAGCCGTCTG CTTCATCATT CCCCCCACCG ACCCCGAGGC CTTGGCTCTT 
GCTGACAGCC TGGGCAGCCA GTACCGAAGT GTGAGGCAGA AGGCGACTGG GAGCAAACAT 
GCACTCCAGC AGCGGCATGA GGTGCTAAGG ACGGAGAACC CTGGAGATGC CTCTGATCTT 
CAGGGACGAC AGCTGCTGGC TGGCTTGGAC AAAGTGGCCA GTGACCTGGA CCGGCAGGAA 
AAGGCTATCA CAGGGATCCT ACGGCCACCA CTTGAGCAGG GACGGGCAAT TGAGGATAGT 
GCGGAGCGGG CCAAGGGTCT CAAGAACATT ACCAATGAGC TGCTACAGAT TGAGCCAGAG 
AAGACGCAGT GCACAGCCGA GTGTGAAGCC TTTGTGCAGG CCCTCCCGGC CAGCGGCACC 
GCACCCTTGC TGAAGACCCG GGTAGAAGAC ACCAACCAGA AATATGAGCG ACTAGTATGG 
TTGCTAGAGG CAGCCCAGGA GAAGGTGGAC GTGGCCAACC GCCTGGAGAA CAGTCTGCAG 
CGGGGCAGGG AGCTGCTGGC CTCATACGAG AACAGGCTGA TTCAGGACGA CACAATGCCT 
GAAAGTGGTC ATGTGTTGGA CAGCAAGAGG CAGGAGCTGG AGGCCATGGC TTCTGAGCTG 
CAGGCCCACA AGTCCCTCCT GGGTGAGGTG GAACAGAACC TACAGGTGGC CAAGCAGTGT 
TCCAGCTCAC TGGCCAGCCG CTTCCAAGAG CACTGCCCTG ACCTGGAGCG GCAGGAGGCC 
GAGGTGCACA AGCTCAACCA ACGTTTCAAC AACCTCAGCC AGCAGGTGGA ACGGAGGGCC 
CAGAGCCTGC AGAGTGCCAG GGCTGCCTAC GATGAATACT GCAGTGGTTA CAACCGTGTG 
CTCCAGTTTT TGGCCAAGAC TCCCAGTTAT GAGCCCCAGG AGACAGACAG CCTCGGCCAG 
ATGGAGACCA AGCTCAAGAA CCAGAAGAAC TTGCTTGATG AGCTAGCAAG CAGGGAACAG 
GAGGTGCAGA AGGTCTATGC AGATTCCCAG CAGTACCAGC AAGCGGTGAA GGACTATGAA 
CTTGAAGCAG AGAAGCTAAG GTCCTTGCTG GACCTGGAGA ATGGACGGAA CAGCCATGTG 
AACAAGAGAG CCAGGCTGCA GTCTCCTGCT GCCAAAGTGA AGGAAGAGGA AGCTGCTCTT 
GCTGCCAAGT TCACAGAGGT TAATGCCATC AACAGACAGA GGCTACAGAA TTTGGAGTTT 
GCACTGAATC TCTTGAGACA GCAACCAGAA GCAGGAGTGA CCCATGAGAC CCTACAAGGG 
GGAAAGCTAA GCTCTGGCAT GGAGGAAACA TGGAAGATTA AGAAGGAACT GGAGGAGGAG 
ATAGAGCGAA GGCAACAGCT GGAAAATGAG GTCAAGAGTG CCCAGGAGGA AATCCAGACC 
CTGAAGGATC AGGGGCCTCA GGAGTCATTG GTGAGGAAGG AGGTGCTGAA GAAGGTGCCA 
GACCCTGCCC TGGAGGAGAG CTTCCAGCAG CTGCAGCAGA CGCTGGCTGA GGAACAGCAC 
AAGAACCAGC TATTGCAGGA GGAGCTGGGA GCACTGCAGC TCCGGCTGCA GGCTCTAGAG 
CAGGAGACCA GGGATGGGGG ACAGGAGTAT GTGGTCAAGG AGGTCTTGCG CATTGAGCCA 
GACAGAGCCC AGGAGGATGA GGTCTTGCAG CTTCGGGAGG AGCTGGAGGC ACTGCGGCGC 
CAGAAAGGCG CCCGAGAGGC TGAAGTGCTA CTCTTACAGC AGCGTGTAGC AGCCCTGGCT 
GCTGAGAAGA GCAGGGTGCA GGAAAAGGTC ACTGAGAGAG AGGTGGTAAA GCTACAGAAT 
GACCCCCAAC TGGAGGCAGA ATACCGGAGG CTGCAGGAGG AGCACCAGCG GGAGGGCACA 
CTCAGGGAGA AGCAGGAGGA GGAGCTGAGT TTCCTACAGG CTAAGCTCAG GAGACTGGAG 
AAAGAACGAG CCATGGCAGA AGGCAAGATC ACTGTCAAAG AGGTGCTCAA GGTAGAGAAA 
GATGCAGCTG TGGAGAGAGA AGTCAATGAC CTCACCCGCC AGTATGAGGA CGAGGCTGCC 
AAGGCACGCT CTGGCCAGCG GGAGAAGACA GAACTTCTCA GAAAGATATG GGCCCTGGAA 
GAGGAAAATG CTAAGGTGGT GGTACAGGAG AAGGTCCGAG AGATCGTGCG GCCAGATCCC 
AAGGCAGAGA GTGAAGTGGC CAACCTCCGC CTGGAGCTGG TGGAGCAGGA GCGCAAATTC 
AGGGGTGCTG AGGAGCAGCT GAAGAGCTAC CAGAGTGAGC TGGAGGCCCT ACGGAACCGT 
GGACCACAGG TAGAAGTCAA AGAGGTGACC AAAGAGGTCA TTAAATATAC AACTGACCCA 
GAGACAGAGC AGGAACTCCA GAGGCTCAGG GAGGAGATCA TGGACAAGAC CAGGTTAATA 
GAAAGGTGTG ACCTAGAGAT TTACCAGCTG AAGCAGGAGA TCCAGGCCCT TAAGGACACC 
AAGCCACAGG TGCAGACTAG AGAGGTAGTC CAGGAGATCC TGCAGTTCCA GGAAGACCCC 
CAAACCAAGA AGGAGGTAGA GTCTCTGCGT ATACAGCTGT CAGAAGAACA GAAGAAACAG 
GTGGACCTGG AAGGGGAGCG GGCATCCCAG GAAGAGAAGA TCAAACGCAA GGAGGAAGAG 
CTGGCACAGG GGAAGGAAAG GGTTGTACGC CAGGAGGTAG TACAGTATGA AGATGAGCCA 
GATTTGCGAG CTGAGGTGAC TGCCTTCACA AACAGCATTG ATGCTGAGCT GCGGCAGATT 
GATAAACTGC ATGTGGAGCT ACGGCGGTTA CAGCATCGCC GAGCAGAGCT GGAGAGGCAG 
TTGGAGGAGC TGGAGCGGGA GAGGCAGGCA CGTAGGGCAG CCGAGCTGGA GGTACAGAGG 
CTGCAACAGC GGCTGGCTGC ATTGGAGCAG GAGGAGGCCA AGACTGGTGA GAAGGTGACC 
CACACACAAA AGGTGGTGCT GCAGCAGGAC CCACAGCAGA CCAGGGAGCA TGCCCTGCTC 
CGAGCCCAGC TGGAGGAAGA GCGGCACCGG AGGCAGCTAC TGGAGGGTGA GCTTGAGCCG 
CTGCGTAGAA AGTTGGCTGC CCTGGAGAAG GCAGAGATCA AAGAAAAGGT GGTCTTCTCT 
GAGAGTGTCC AGGTAGAAAA AGGTGACACT GAGCAAGAGA TCCAGCGCCT CAAGAAGAGC 
CTGGAAGAGG AGAGCCAGAG CAAGAGGGAG CTGGATTCAG AGGTGACCCG GCTAGAAGCC 
AAGTTATCTG AGCTAGAATT CTATAATTCC AAGTCATCCA AGGAACTGGA CTTCCTGAGA 
GAAGAAAACC ACAAGCTACA GCTAGAGCGG CAAAACCTGC AGCTAGAGAC CCGGAGACTC 
CAATCAGAGA TTGAGATGGC AGCAACAGAA ACTCGAGATC TGAAGAACAT AACCACGATA 
GACTCTGGGA CCCACCTAAA CTCTAGGCTG TGGTCCCTAG AGAAGGAACT GGATGACCTC 
AAGAAGATGT CCAAGGACAA GGATCTGGAG ATCGATGAAC TACAGAGGCG CCTGGGCTCT 
GTGGCTGTCA AGAGAGAGCA GAGGGAGAAC CACCTGAGGC GCTCCATAGT GGTCATAGAC 
CCTGACACAG GTCGTGAGCT GTCCCCAGAG GAGGCTCACC GGGCTGGGCT TATCGACTGG 
AAAATGTTTG TGAAGCTCAG AAGCCAGGAG TGCGACTGGG AGGAGATATC AGTGAAAGGT 
CCTAATGGGG AGTCATCAGT AATTCATGAC AGGAAGTCAG GCAAGAAGTT CTCCATTGAG 
GACGCTCTTC AGAGTGGCAG GCTAACCGCT GCTCAATATG ACCGCTATGT CAACAAGGAT 
ATGTCCATTC AGGAGCTGGC AGTCTTGGTG TCTGGGCAGA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032935.2
CDS	139..5403
Translation	MHSLFRKRNKGKYSPTVQTRSISNKELSDLIEQLQKNADQVERNIVDTEAKMQSDLARMQEGQLPEHRDAALQNVSDSEKLLYVLEADSAIAKHMKHPQGDMIAEDIRQLKERVTNLRGKHKQMYSLAVKEADPRVNWDTLVDEKLDKLSSQSFGTDLPLVDSQVEQHNIFHNEVKAIGPHLAKDKEQNSELQAKYQKLLTASQARQQHLSSLQDYMQRCTNELYWLDQQAKGRMQYDWSDRNLDYPSRRRQYENFINRNLEAKEERINKLHTEGDQLLTAEHPGRNSIEAHMEAVHAEWKEYLNLLICEESHLKYMEDYHQFHKDMKDAQELLRKVDSDLNQKYSPDFKDRYQIELLLRELDDQEKALDKYEDVVRGLQRRGQQVVPLKYRRETPLKPIPVEALCDFESDQGLISRGYSYTLQKNNGESWELTDSTGKKLAAPAVCFIIPPTDPEALALADSLGSQYRSVRQKATGSKHALQQRHEVLRTENPGDASDLQGRQLLAGLDKVASDLDRQEKAITGILRPPLEQGRAIEDSAERAKGLKNITNELLQIEPEKTQCTAECEAFVQALPASGTAPLLKTRVEDTNQKYERLVWLLEAAQEKVDVANRLENSLQRGRELLASYENRLIQDDTMPESGHVLDSKRQELEAMASELQAHKSLLGEVEQNLQVAKQCSSSLASRFQEHCPDLERQEAEVHKLNQRFNNLSQQVERRAQSLQSARAAYDEYCSGYNRVLQFLAKTPSYEPQETDSLGQMETKLKNQKNLLDELASREQEVQKVYADSQQYQQAVKDYELEAEKLRSLLDLENGRNSHVNKRARLQSPAAKVKEEEAALAAKFTEVNAINRQRLQNLEFALNLLRQQPEAGVTHETLQGGKLSSGMEETWKIKKELEEEIERRQQLENEVKSAQEEIQTLKDQGPQESLVRKEVLKKVPDPALEESFQQLQQTLAEEQHKNQLLQEELGALQLRLQALEQETRDGGQEYVVKEVLRIEPDRAQEDEVLQLREELEALRRQKGAREAEVLLLQQRVAALAAEKSRVQEKVTEREVVKLQNDPQLEAEYRRLQEEHQREGTLREKQEEELSFLQAKLRRLEKERAMAEGKITVKEVLKVEKDAAVEREVNDLTRQYEDEAAKARSGQREKTELLRKIWALEEENAKVVVQEKVREIVRPDPKAESEVANLRLELVEQERKFRGAEEQLKSYQSELEALRNRGPQVEVKEVTKEVIKYTTDPETEQELQRLREEIMDKTRLIERCDLEIYQLKQEIQALKDTKPQVQTREVVQEILQFQEDPQTKKEVESLRIQLSEEQKKQVDLEGERASQEEKIKRKEEELAQGKERVVRQEVVQYEDEPDLRAEVTAFTNSIDAELRQIDKLHVELRRLQHRRAELERQLEELERERQARRAAELEVQRLQQRLAALEQEEAKTGEKVTHTQKVVLQQDPQQTREHALLRAQLEEERHRRQLLEGELEPLRRKLAALEKAEIKEKVVFSESVQVEKGDTEQEIQRLKKSLEEESQSKRELDSEVTRLEAKLSELEFYNSKSSKELDFLREENHKLQLERQNLQLETRRLQSEIEMAATETRDLKNITTIDSGTHLNSRLWSLEKELDDLKKMSKDKDLEIDELQRRLGSVAVKREQRENHLRRSIVVIDPDTGRELSPEEAHRAGLIDWKMFVKLRSQECDWEEISVKGPNGESSVIHDRKSGKKFSIEDALQSGRLTAAQYDRYVNKDMSIQELAVLVSGQK

Target ORF information:

RefSeq Version	NM_008909.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus periplakin (Ppl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008909.2

ORF Insert Sequence:

ATGCACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCAACGGT GCAGACGCGG 
AGCATCTCCA ACAAGGAACT CTCAGATCTG ATTGAGCAGC TGCAGAAGAA TGCAGACCAG 
GTGGAAAGGA ACATTGTGGA CACCGAGGCC AAGATGCAGA GCGACTTGGC CCGGATGCAA 
GAGGGGCAGC TCCCTGAGCA CCGGGACGCG GCCCTGCAGA ACGTGTCTGA CTCAGAAAAG 
CTGCTGTACG TGCTGGAGGC AGATTCGGCC ATCGCCAAGC ACATGAAGCA CCCACAGGGG 
GACATGATCG CCGAGGACAT CCGCCAGCTG AAAGAGCGCG TGACCAACCT GCGGGGGAAA 
CACAAGCAGA TGTACAGCCT GGCAGTGAAA GAGGCCGACC CGAGAGTCAA CTGGGACACA 
CTGGTGGACG AGAAGCTGGA CAAGCTGAGC AGCCAGAGCT TTGGGACTGA CCTTCCTCTG 
GTGGATAGCC AGGTGGAGCA ACACAACATC TTCCACAATG AAGTCAAAGC CATCGGGCCC 
CACCTGGCCA AGGACAAGGA GCAGAACAGT GAACTCCAAG CCAAGTATCA GAAGTTGCTG 
ACAGCATCAC AGGCACGGCA GCAGCACCTG AGCTCGCTGC AGGATTACAT GCAGCGCTGC 
ACCAATGAGC TGTACTGGCT GGACCAGCAA GCCAAGGGCC GCATGCAGTA TGACTGGAGC 
GACCGCAACC TCGACTACCC CAGCCGCCGC CGCCAGTACG AGAATTTCAT CAACCGGAAC 
CTGGAGGCCA AAGAAGAAAG AATCAACAAA CTGCACACTG AAGGTGACCA ACTGCTGACA 
GCTGAGCACC CAGGGAGGAA CTCCATTGAG GCACACATGG AGGCTGTGCA TGCTGAGTGG 
AAGGAGTACC TGAACCTGCT CATCTGTGAG GAGAGCCATC TGAAATACAT GGAAGACTAC 
CACCAATTTC ACAAAGACAT GAAGGATGCT CAGGAGCTGC TACGCAAGGT GGACTCAGAC 
TTGAACCAGA AATATAGCCC TGACTTCAAA GACCGGTATC AGATAGAGCT GTTGCTTCGG 
GAACTGGATG ACCAGGAGAA GGCTCTGGAC AAGTATGAGG ATGTGGTGCG AGGGCTGCAG 
AGGCGAGGGC AGCAAGTGGT GCCACTCAAG TACCGCCGCG AGACGCCGCT CAAGCCCATC 
CCTGTGGAGG CTCTCTGTGA CTTTGAGAGT GACCAGGGCC TCATCTCGCG GGGCTATAGC 
TACACACTGC AGAAGAACAA CGGGGAGAGC TGGGAGCTGA CAGACAGCAC TGGGAAGAAG 
CTGGCTGCCC CAGCCGTCTG CTTCATCATT CCCCCCACCG ACCCCGAGGC CTTGGCTCTT 
GCTGACAGCC TGGGCAGCCA GTACCGAAGT GTGAGGCAGA AGGCGACTGG GAGCAAACAT 
GCACTCCAGC AGCGGCATGA GGTGCTAAGG ACGGAGAACC CTGGAGATGC CTCTGATCTT 
CAGGGACGAC AGCTGCTGGC TGGCTTGGAC AAAGTGGCCA GTGACCTGGA CCGGCAGGAA 
AAGGCTATCA CAGGGATCCT ACGGCCACCA CTTGAGCAGG GACGGGCAAT TGAGGATAGT 
GCGGAGCGGG CCAAGGGTCT CAAGAACATT ACCAATGAGC TGCTACAGAT TGAGCCAGAG 
AAGACGCAGT GCACAGCCGA GTGTGAAGCC TTTGTGCAGG CCCTCCCGGC CAGCGGCACC 
GCACCCTTGC TGAAGACCCG GGTAGAAGAC ACCAACCAGA AATATGAGCG ACTAGTATGG 
TTGCTAGAGG CAGCCCAGGA GAAGGTGGAC GTGGCCAACC GCCTGGAGAA CAGTCTGCAG 
CGGGGCAGGG AGCTGCTGGC CTCATACGAG AACAGGCTGA TTCAGGACGA CACAATGCCT 
GAAAGTGGTC ATGTGTTGGA CAGCAAGAGG CAGGAGCTGG AGGCCATGGC TTCTGAGCTG 
CAGGCCCACA AGTCCCTCCT GGGTGAGGTG GAACAGAACC TACAGGTGGC CAAGCAGTGT 
TCCAGCTCAC TGGCCAGCCG CTTCCAAGAG CACTGCCCTG ACCTGGAGCG GCAGGAGGCC 
GAGGTGCACA AGCTCAACCA ACGTTTCAAC AACCTCAGCC AGCAGGTGGA ACGGAGGGCC 
CAGAGCCTGC AGAGTGCCAG GGCTGCCTAC GATGAATACT GCAGTGGTTA CAACCGTGTG 
CTCCAGTTTT TGGCCAAGAC TCCCAGTTAT GAGCCCCAGG AGACAGACAG CCTCGGCCAG 
ATGGAGACCA AGCTCAAGAA CCAGAAGAAC TTGCTTGATG AGCTAGCAAG CAGGGAACAG 
GAGGTGCAGA AGGTCTATGC AGATTCCCAG CAGTACCAGC AAGCGGTGAA GGACTATGAA 
CTTGAAGCAG AGAAGCTAAG GTCCTTGCTG GACCTGGAGA ATGGACGGAA CAGCCATGTG 
AACAAGAGAG CCAGGCTGCA GTCTCCTGCT GCCAAAGTGA AGGAAGAGGA AGCTGCTCTT 
GCTGCCAAGT TCACAGAGGT TAATGCCATC AACAGACAGA GGCTACAGAA TTTGGAGTTT 
GCACTGAATC TCTTGAGACA GCAACCAGAA GCAGGAGTGA CCCATGAGAC CCTACAAGGG 
GGAAAGCTAA GCTCTGGCAT GGAGGAAACA TGGAAGATTA AGAAGGAACT GGAGGAGGAG 
ATAGAGCGAA GGCAACAGCT GGAAAATGAG GTCAAGAGTG CCCAGGAGGA AATCCAGACC 
CTGAAGGATC AGGGGCCTCA GGAGTCATTG GTGAGGAAGG AGGTGCTGAA GAAGGTGCCA 
GACCCTGCCC TGGAGGAGAG CTTCCAGCAG CTGCAGCAGA CGCTGGCTGA GGAACAGCAC 
AAGAACCAGC TATTGCAGGA GGAGCTGGGA GCACTGCAGC TCCGGCTGCA GGCTCTAGAG 
CAGGAGACCA GGGATGGGGG ACAGGAGTAT GTGGTCAAGG AGGTCTTGCG CATTGAGCCA 
GACAGAGCCC AGGAGGATGA GGTCTTGCAG CTTCGGGAGG AGCTGGAGGC ACTGCGGCGC 
CAGAAAGGCG CCCGAGAGGC TGAAGTGCTA CTCTTACAGC AGCGTGTAGC AGCCCTGGCT 
GCTGAGAAGA GCAGGGTGCA GGAAAAGGTC ACTGAGAGAG AGGTGGTAAA GCTACAGAAT 
GACCCCCAAC TGGAGGCAGA ATACCGGAGG CTGCAGGAGG AGCACCAGCG GGAGGGCACA 
CTCAGGGAGA AGCAGGAGGA GGAGCTGAGT TTCCTACAGG CTAAGCTCAG GAGACTGGAG 
AAAGAACGAG CCATGGCAGA AGGCAAGATC ACTGTCAAAG AGGTGCTCAA GGTAGAGAAA 
GATGCAGCTG TGGAGAGAGA AGTCAATGAC CTCACCCGCC AGTATGAGGA CGAGGCTGCC 
AAGGCACGCT CTGGCCAGCG GGAGAAGACA GAACTTCTCA GAAAGATATG GGCCCTGGAA 
GAGGAAAATG CTAAGGTGGT GGTACAGGAG AAGGTCCGAG AGATCGTGCG GCCAGATCCC 
AAGGCAGAGA GTGAAGTGGC CAACCTCCGC CTGGAGCTGG TGGAGCAGGA GCGCAAATTC 
AGGGGTGCTG AGGAGCAGCT GAAGAGCTAC CAGAGTGAGC TGGAGGCCCT ACGGAACCGT 
GGACCACAGG TAGAAGTCAA AGAGGTGACC AAAGAGGTCA TTAAATATAC AACTGACCCA 
GAGACAGAGC AGGAACTCCA GAGGCTCAGG GAGGAGATCA TGGACAAGAC CAGGTTAATA 
GAAAGGTGTG ACCTAGAGAT TTACCAGCTG AAGCAGGAGA TCCAGGCCCT TAAGGACACC 
AAGCCACAGG TGCAGACTAG AGAGGTAGTC CAGGAGATCC TGCAGTTCCA GGAAGACCCC 
CAAACCAAGA AGGAGGTAGA GTCTCTGCGT ATACAGCTGT CAGAAGAACA GAAGAAACAG 
GTGGACCTGG AAGGGGAGCG GGCATCCCAG GAAGAGAAGA TCAAACGCAA GGAGGAAGAG 
CTGGCACAGG GGAAGGAAAG GGTTGTACGC CAGGAGGTAG TACAGTATGA AGATGAGCCA 
GATTTGCGAG CTGAGGTGAC TGCCTTCACA AACAGCATTG ATGCTGAGCT GCGGCAGATT 
GATAAACTGC ATGTGGAGCT ACGGCGGTTA CAGCATCGCC GAGCAGAGCT GGAGAGGCAG 
TTGGAGGAGC TGGAGCGGGA GAGGCAGGCA CGTAGGGCAG CCGAGCTGGA GGTACAGAGG 
CTGCAACAGC GGCTGGCTGC ATTGGAGCAG GAGGAGGCCA AGACTGGTGA GAAGGTGACC 
CACACACAAA AGGTGGTGCT GCAGCAGGAC CCACAGCAGA CCAGGGAGCA TGCCCTGCTC 
CGAGCCCAGC TGGAGGAAGA GCGGCACCGG AGGCAGCTAC TGGAGGGTGA GCTTGAGCCG 
CTGCGTAGAA AGTTGGCTGC CCTGGAGAAG GCAGAGATCA AAGAAAAGGT GGTCTTCTCT 
GAGAGTGTCC AGGTAGAAAA AGGTGACACT GAGCAAGAGA TCCAGCGCCT CAAGAAGAGC 
CTGGAAGAGG AGAGCCAGAG CAAGAGGGAG CTGGATTCAG AGGTGACCCG GCTAGAAGCC 
AAGTTATCTG AGCTAGAATT CTATAATTCC AAGTCATCCA AGGAACTGGA CTTCCTGAGA 
GAAGAAAACC ACAAGCTACA GCTAGAGCGG CAAAACCTGC AGCTAGAGAC CCGGAGACTC 
CAATCAGAGA TTGAGATGGC AGCAACAGAA ACTCGAGATC TGAAGAACAT AACCACGATA 
GACTCTGGGA CCCACCTAAA CTCTAGGCTG TGGTCCCTAG AGAAGGAACT GGATGACCTC 
AAGAAGATGT CCAAGGACAA GGATCTGGAG ATCGATGAAC TACAGAGGCG CCTGGGCTCT 
GTGGCTGTCA AGAGAGAGCA GAGGGAGAAC CACCTGAGGC GCTCCATAGT GGTCATAGAC 
CCTGACACAG GTCGTGAGCT GTCCCCAGAG GAGGCTCACC GGGCTGGGCT TATCGACTGG 
AAAATGTTTG TGAAGCTCAG AAGCCAGGAG TGCGACTGGG AGGAGATATC AGTGAAAGGT 
CCTAATGGGG AGTCATCAGT AATTCATGAC AGGAAGTCAG GCAAGAAGTT CTCCATTGAG 
GACGCTCTTC AGAGTGGCAG GCTAACCGCT GCTCAATATG ACCGCTATGT CAACAAGGAT 
ATGTCCATTC AGGAGCTGGC AGTCTTGGTG TCTGGGCAGA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Increased Bacterial Load and Expression of Antimicrobial Peptides in Skin of Barrier-Deficient Mice with Reduced Cancer Susceptibility.
The Journal of investigative dermatology136(1)99-106(2016 Jan)
Natsuga K,Cipolat S,Watt FM

Epidermal barrier defects link atopic dermatitis with altered skin cancer susceptibility.
eLife3e01888(2014 May)
Cipolat S,Hoste E,Natsuga K,Quist SR,Watt FM

Annexin A9 is a periplakin interacting partner in membrane-targeted cytoskeletal linker protein complexes.
FEBS letters586(19)3090-6(2012 Sep)
Boczonadi V,M??tt? A

Exons 5-15 of kazrin are dispensable for murine epidermal morphogenesis and homeostasis.
The Journal of investigative dermatology132(8)1977-87(2012 Aug)
Chhatriwala MK,Cipolat S,Sevilla LM,Nachat R,Watt FM

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Ppl cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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