PPL gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005224663.3	XP_005224720.1	periplakin isoform X3
XM_005224662.4	XP_005224719.1	periplakin isoform X1
XM_024985416.1	XP_024841184.1	periplakin isoform X4
XM_010799955.2	XP_010798257.2	periplakin isoform X3
XM_005224662.3	XP_005224719.1	periplakin isoform X1
XM_015469294.1	XP_015324780.1	periplakin isoform X2
XM_002697888.5	XP_002697934.3	periplakin isoform X2
XM_010799950.2	XP_010798252.2	periplakin isoform X1
XM_002697888.6	XP_002697934.3	periplakin isoform X2
XM_005224663.4	XP_005224720.1	periplakin isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa230667	XM_024985416.1 Latest version!	Bos taurus periplakin (PPL), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa32912	XM_005224662.4 Latest version!	Bos taurus periplakin (PPL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa32912	XM_010799950.2 Latest version!	Bos taurus periplakin (PPL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa32913	XM_015469294.1 Latest version!	Bos taurus periplakin (PPL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa32913	XM_002697888.6 Latest version!	Bos taurus periplakin (PPL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa32914	XM_010799955.2 Latest version!	Bos taurus periplakin (PPL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa32914	XM_005224663.4 Latest version!	Bos taurus periplakin (PPL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
View Old Accession Versions >>
OBa32912	XM_005224662.3	Bos taurus periplakin (PPL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa32913	XM_002697888.5	Bos taurus periplakin (PPL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa32914	XM_005224663.3	Bos taurus periplakin (PPL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

PPL ( XM_024985416.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024841184.1 Bos taurus periplakin (PPL), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa230667
Clone ID Related Accession (Same CDS sequence)	XM_024985416.1
Accession Version	XM_024985416.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	periplakin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGATG CCCAGGAGCT GCTTCGCAAG GTGGACTCAG ACCTGAACCA GAAATACAGC 
CCTGACTTCA AGGACCGATA CCAGATTGAG CTGCTGCTGC GGGAGCTGGA TGACCAGGAG 
AAGGCTCTGG ACAAGTACCA GCATGTGGTG CGGGGGCTGC AGAAGCGAGG GCAGCAGGTG 
GTGCCCCTCA AGTACCGCCG GGAGACGCCC CTCAAGCCCA TCCCCGTGGA GGCGCTCTGT 
GACTTCGAGG GTGACCAGGG CCTGATCTCT CGGGGCTACA GCTACACCCT GCAGAGGAAC 
AATGGGGAGA GCTGGGACCT CACCGACAGT GCGGGGAACA AGCTGACCGC CCCGGCCGTC 
TGCTTCATGA TCCCGCCCAC TGACCCCGAG GCCCTGGCTC TGGCTGAAAG CCTGGGCAGC 
CAGTACCAGA GCCTGCGGCA GAAGGCAGCT GGGAGCAGAA AGGCGCTCCA GGAGCAGCAT 
GAGGTGCTGA AGGCAGAGAG CTCTGGAGCA GATGCCTCGG ACCTGCAGGG CCGCCAGCTG 
CTGGCCGGCT TGGACAAGGT GGCCAGCGAC CTGGACCGGC AGGAGAAGGC CATCACGGCC 
ATCCTGCGGC CGCCGCTGGA GCAGGGCCGG GCTGTGCAGG ACAGCGCCGA GAGGGCCAAG 
GACCTCAAGA ACATCACCAA CGAGCTGCTG CGGATCGAGC CCGAGAAGGC GCGCAGCACG 
GCCGAGGGCG AGGCGTTCGT GCGGGCCCTC CCGGACAGCG GCAGCACCGC CCTGCTGAGG 
ACGCGGGTGG AGGACACCCA GCGCAGATAC GAGCGCCTGG TGCAGCTGCT GGAAGCCGCC 
CAGGAGAAGC TCGACATCGC CAACCGCCTG GAGAAGAGCC TGCAGCAGGG CCGGGAGATG 
CTGGCCACGT ATGAGACCCA GCTGGCCCAG GAGGACACGG TGCCCGAGAG TGGCCAGGCC 
CTGGACAGCA GGAGGCAGGA GCTGGCGGCC ATGGCCTCCG AGCTGCAGGC CCGGAAGGCC 
CTCCTCGGCG AGGTGCAGCG GAACCTGCAG GCCGCCAAGC AGTGCTCGGG CTCGCTGGCC 
AGCCGCTTCC AGGAGCACTG CCCGGACCTG GAGCGCCAGG AGGCCGAGGT GCACAAGCTG 
TGCCAGCGCT TCGACGGCCT CTGCCAGCAG GCGGAGCTCA GGGTCCAGAG CCTGCAGAGC 
GCCCGGGCTG AGCACGACGC CTTCTGCAGC GGCCGTGACC GCCTGCTTCA GTTCCTGTCC 
CACATCCCCA GCTACGAGCC CCAGGAGACG GACGGCCTCG GCCAAGTGGA GACCAAGCTG 
AACAACCAGA AGAACCTACT AGATGAAATA GCAAGAAGGG AAGAGGAAGT GCAGAAAGTC 
CACACGCACT CCCAGCAGTA CCAGCAGGCT GTCAAGGACT ACGAGTTGGA AGCAGAGAAA 
CTCAGGTCCC TCCTTGACTT GGAGAATGGA AGGAGCAGCC ACGTGAGCAA GCGGGCCAGG 
CTCCAGTCCC CTGCCACCAA AGTGAGGGAA GAGGAAGCAG CTCTTGCTGC CAAGTTTACG 
GAGGTTAATG CCATCAACAG ACAGAGACTG CAGAACCTGG AGTTTGCACT GACCCTCCTG 
AGACAGCAGC TGGAGGCAGG TGCAAGCCAG GAGGCCCTGC CCGGGAGCAA GCCAGGCCCT 
GGAGCAGAGG AGACGTGGAA GATTCAGGAG GAGCTGGATG AGGAGATGGA GCGGCGGCAG 
CAGCTGGAGC ACGAGGTCCG GAGCGCCCAG GAGGAGATCC GGGCCCTGCG GAGCCAGAGC 
CCGCAGGAGG CGGTGGTGAC CAGGGAGGTG CTCAGGAAGG TGCCGGACCC AGCACTGGAG 
GAGAGCTTCC AGCAGGCGCA GCAGACCCTG GCCGAGGAGC AGCACAAGAA CCGGCTGCTG 
CAGGGGGAGG TGGAGGCGCT GCGGCTGCGG CTGCGTGCCC TGGAGGAGGC GGCCCGGGCC 
GGGGGCCAGG AGTACGTGGT CAAGGAGGTA CTGCGCATTG AGCCGGATCG GGCCCAGGCT 
GACGAGGTCC TGAGGCTGCG GGGGGAGCTG GAGGTGCTGC GGCGGCAGAA GGGCGCCCGC 
GAGGCGGAGG CGCTTCTCCT GCAGCAGCGC GTGGCCGCCC TGGCCGAGGA GAAGCGGCGG 
GCGCGGGAGG TGGTCACAGA GAAGGAGGTG GTCAAGGTGC ACAATGACCC CCAGCTGGAG 
GCCGAGTTCC GGCAGCTGCA GGAGGACCAG CAGCGGGAGC AGGGGCTCCG GGAGAAGCAG 
GAGGAGGAGC TGGGCTTCCT GCAGGACAAG CTCAAGAGGC TGGAGAAGGA ACGGGCCATG 
GCCGAGGGCA AGATCACAGT CAAGGAGGTG CTCAAGGTGG AGAAGGACGT GGCGGCTGAG 
CGGGAGGTGG GCGCCCTCCA GCGCCGGTAT GAGGATGAGG CCGCCAGGGC CCGTGCCAGC 
CAGAGGGAGA AGACCGAGCT GCTCCGGAAG ATCTGGGCCC TGGAGGAGGA GAACGCTAAG 
GTGGTTGTGC AAGAGAAGGT GCGCGAGATT GTCCGGCCTG ACCCCAAGGC TGAGAGTGAG 
GTGGCCAACC TCCGCCTGGA GCTGGTAGAG CAGGAGCGCA AGTACCGGGG GGCTGAGGAG 
CAGCTCAAGA GCTACCAGAG CGAGCTGGAG GCGCTTCGGA GGCGAGGCCC CCAGGTAGAG 
GTCAAGGAGG TGACCAAGGA GGTCATCAAG TACACGACGG ACCCTGAGAC GGAGAAGGAG 
CTCCAGAGGC TCAGGGAGGA GATCGTGGAC AAGACCCGCC TCATCGAGAG GTGTGACCTG 
GAGATCTACC AGCTGAAGCA GGAGATCCAG TCCCTCAAAG AGGCCAAGCC CCAGGTGCAG 
ACCAAGGAGG TGGTCCAGGA GATCTTGCAG TTCCAGGAAG ACCCCCAGAC CAAAGAGGAA 
GTGGCGGCTC TGAGGGCCCG GCTGGCTGAG GAGCACAAGA AGCAGGTAGA CCTGGAACGG 
GAATGCGTGT CACAGGAGGA GAAGATCCAG GAGAAGGAGG AGGAGCTGGC CCGCGGGAAG 
GAGGTGGTGG TGCAGCAGGA GGTGGTCAGG TACGAGGAGG AGCCGGGCCT CCGGGCGGAA 
GTGAGCGCCT TCACCGAGAG CATCGATGCA GAACTGCGGC AGATCGACGG CCTCCGCGGC 
GAGCTGCGGC GGCTGCAGCG CCGGCGAGCC GAGCTGGAGC ACCAGCTGGA GGAGCTGGAG 
CGCGAGCGGC AGGCGCGCCG CGAGGCTGAG CTGGAGGTGC AGCACCTCAA GCAACAGCTG 
GCCCAGCTGG AGGCGCAGGA GGGCGAGGCC CACGAGAAGG TGACCCTCAC ACAGAAGGTG 
GTGCTGCAGC AGGACCCCCA GCAGGCCCGG GAGCATGCCC TCCTGGCCCT GCAGCTGGAG 
GAAGAGCGGC ACCGCCGGCA GGGCCTGGAG CGCGAGCTCG AGACCCTAAA GCAGCAGCTG 
GAGCACCTGG AGAAGATGGA GGTCAAGGAG AAGGTGGTCT TCTCCGAGAG CGTCCAGGTG 
GAGAAAGGCG ACACCGAGCA GGAGATTGAG AGGCTCAGGG GCAGTCTGGA GGCTGAGAGC 
CGAGGCAAGA GGGAGCTGGA CGCCGAGGTG AGCCGGCTGG AGGCCAAGCT GTCGGAGCTG 
GAGTTCTGCA ACTCTAAGTC ATCCAAGGAG CTGGACTTCC TGAGGGAGGA GAACCACAAG 
CTGCAGCTGG AGCGGCAGAG CCTGCAGCTC GAGGCCCGCA GGCTGCAGTC GGAGATCGAG 
ATGGCCAAGG CCGAGGCGCA GAGCCTAAGA AGCCTGCCCA CAACGACTGG AGCCGACCCC 
GGGGCGCAGC TCGACTCACG CCTGCGGTCC CTGGAGCGGG AGCTGGACGA CCTCAGGAGG 
CTCTCCAGGG ACAAAGACCA GGAGATCGAA GAGCTGCAGC GTCGCCTGGG GTCCGTGGCC 
ATCAAGCGGG AGCAGAGGGA GAACCACCTG CGGCGCTCCA TTGTGGTCAT CGACCCTGAC 
TCGGGCCGTG AGCTGTCCCC CGAGGAGGCC CACCGCGCCG GCCTCATCGA CTGGAACATG 
TTTGTCAAGC TCAGGAGCCA GGAGTGCGAC TGGGAGGAGA TCTCCGTCAA GGGCCCCGGA 
GGCGAGTCCT CCGTGATCCA TGACAGGAAG TCCGGCAGGA AGTTCTCCAT TGAAGAGGCC 
CTGCAGAGCG GGCGGCTGAC CCCCGCGCAG TACGACCGCT ACGTCAACAA GAGCATGTCC 
ATCCAGGAGC TGGCCGTCCT GGTGTCAGGG CAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024841184.1
CDS	229..4527
Translation	MKDAQELLRKVDSDLNQKYSPDFKDRYQIELLLRELDDQEKALDKYQHVVRGLQKRGQQVVPLKYRRETPLKPIPVEALCDFEGDQGLISRGYSYTLQRNNGESWDLTDSAGNKLTAPAVCFMIPPTDPEALALAESLGSQYQSLRQKAAGSRKALQEQHEVLKAESSGADASDLQGRQLLAGLDKVASDLDRQEKAITAILRPPLEQGRAVQDSAERAKDLKNITNELLRIEPEKARSTAEGEAFVRALPDSGSTALLRTRVEDTQRRYERLVQLLEAAQEKLDIANRLEKSLQQGREMLATYETQLAQEDTVPESGQALDSRRQELAAMASELQARKALLGEVQRNLQAAKQCSGSLASRFQEHCPDLERQEAEVHKLCQRFDGLCQQAELRVQSLQSARAEHDAFCSGRDRLLQFLSHIPSYEPQETDGLGQVETKLNNQKNLLDEIARREEEVQKVHTHSQQYQQAVKDYELEAEKLRSLLDLENGRSSHVSKRARLQSPATKVREEEAALAAKFTEVNAINRQRLQNLEFALTLLRQQLEAGASQEALPGSKPGPGAEETWKIQEELDEEMERRQQLEHEVRSAQEEIRALRSQSPQEAVVTREVLRKVPDPALEESFQQAQQTLAEEQHKNRLLQGEVEALRLRLRALEEAARAGGQEYVVKEVLRIEPDRAQADEVLRLRGELEVLRRQKGAREAEALLLQQRVAALAEEKRRAREVVTEKEVVKVHNDPQLEAEFRQLQEDQQREQGLREKQEEELGFLQDKLKRLEKERAMAEGKITVKEVLKVEKDVAAEREVGALQRRYEDEAARARASQREKTELLRKIWALEEENAKVVVQEKVREIVRPDPKAESEVANLRLELVEQERKYRGAEEQLKSYQSELEALRRRGPQVEVKEVTKEVIKYTTDPETEKELQRLREEIVDKTRLIERCDLEIYQLKQEIQSLKEAKPQVQTKEVVQEILQFQEDPQTKEEVAALRARLAEEHKKQVDLERECVSQEEKIQEKEEELARGKEVVVQQEVVRYEEEPGLRAEVSAFTESIDAELRQIDGLRGELRRLQRRRAELEHQLEELERERQARREAELEVQHLKQQLAQLEAQEGEAHEKVTLTQKVVLQQDPQQAREHALLALQLEEERHRRQGLERELETLKQQLEHLEKMEVKEKVVFSESVQVEKGDTEQEIERLRGSLEAESRGKRELDAEVSRLEAKLSELEFCNSKSSKELDFLREENHKLQLERQSLQLEARRLQSEIEMAKAEAQSLRSLPTTTGADPGAQLDSRLRSLERELDDLRRLSRDKDQEIEELQRRLGSVAIKREQRENHLRRSIVVIDPDSGRELSPEEAHRAGLIDWNMFVKLRSQECDWEEISVKGPGGESSVIHDRKSGRKFSIEEALQSGRLTPAQYDRYVNKSMSIQELAVLVSGQK

Target ORF information:

RefSeq Version	XM_024985416.1
Organism	Bos taurus(cattle)
Definition	Bos taurus periplakin (PPL), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985416.1

ORF Insert Sequence:

ATGAAGGATG CCCAGGAGCT GCTTCGCAAG GTGGACTCAG ACCTGAACCA GAAATACAGC 
CCTGACTTCA AGGACCGATA CCAGATTGAG CTGCTGCTGC GGGAGCTGGA TGACCAGGAG 
AAGGCTCTGG ACAAGTACCA GCATGTGGTG CGGGGGCTGC AGAAGCGAGG GCAGCAGGTG 
GTGCCCCTCA AGTACCGCCG GGAGACGCCC CTCAAGCCCA TCCCCGTGGA GGCGCTCTGT 
GACTTCGAGG GTGACCAGGG CCTGATCTCT CGGGGCTACA GCTACACCCT GCAGAGGAAC 
AATGGGGAGA GCTGGGACCT CACCGACAGT GCGGGGAACA AGCTGACCGC CCCGGCCGTC 
TGCTTCATGA TCCCGCCCAC TGACCCCGAG GCCCTGGCTC TGGCTGAAAG CCTGGGCAGC 
CAGTACCAGA GCCTGCGGCA GAAGGCAGCT GGGAGCAGAA AGGCGCTCCA GGAGCAGCAT 
GAGGTGCTGA AGGCAGAGAG CTCTGGAGCA GATGCCTCGG ACCTGCAGGG CCGCCAGCTG 
CTGGCCGGCT TGGACAAGGT GGCCAGCGAC CTGGACCGGC AGGAGAAGGC CATCACGGCC 
ATCCTGCGGC CGCCGCTGGA GCAGGGCCGG GCTGTGCAGG ACAGCGCCGA GAGGGCCAAG 
GACCTCAAGA ACATCACCAA CGAGCTGCTG CGGATCGAGC CCGAGAAGGC GCGCAGCACG 
GCCGAGGGCG AGGCGTTCGT GCGGGCCCTC CCGGACAGCG GCAGCACCGC CCTGCTGAGG 
ACGCGGGTGG AGGACACCCA GCGCAGATAC GAGCGCCTGG TGCAGCTGCT GGAAGCCGCC 
CAGGAGAAGC TCGACATCGC CAACCGCCTG GAGAAGAGCC TGCAGCAGGG CCGGGAGATG 
CTGGCCACGT ATGAGACCCA GCTGGCCCAG GAGGACACGG TGCCCGAGAG TGGCCAGGCC 
CTGGACAGCA GGAGGCAGGA GCTGGCGGCC ATGGCCTCCG AGCTGCAGGC CCGGAAGGCC 
CTCCTCGGCG AGGTGCAGCG GAACCTGCAG GCCGCCAAGC AGTGCTCGGG CTCGCTGGCC 
AGCCGCTTCC AGGAGCACTG CCCGGACCTG GAGCGCCAGG AGGCCGAGGT GCACAAGCTG 
TGCCAGCGCT TCGACGGCCT CTGCCAGCAG GCGGAGCTCA GGGTCCAGAG CCTGCAGAGC 
GCCCGGGCTG AGCACGACGC CTTCTGCAGC GGCCGTGACC GCCTGCTTCA GTTCCTGTCC 
CACATCCCCA GCTACGAGCC CCAGGAGACG GACGGCCTCG GCCAAGTGGA GACCAAGCTG 
AACAACCAGA AGAACCTACT AGATGAAATA GCAAGAAGGG AAGAGGAAGT GCAGAAAGTC 
CACACGCACT CCCAGCAGTA CCAGCAGGCT GTCAAGGACT ACGAGTTGGA AGCAGAGAAA 
CTCAGGTCCC TCCTTGACTT GGAGAATGGA AGGAGCAGCC ACGTGAGCAA GCGGGCCAGG 
CTCCAGTCCC CTGCCACCAA AGTGAGGGAA GAGGAAGCAG CTCTTGCTGC CAAGTTTACG 
GAGGTTAATG CCATCAACAG ACAGAGACTG CAGAACCTGG AGTTTGCACT GACCCTCCTG 
AGACAGCAGC TGGAGGCAGG TGCAAGCCAG GAGGCCCTGC CCGGGAGCAA GCCAGGCCCT 
GGAGCAGAGG AGACGTGGAA GATTCAGGAG GAGCTGGATG AGGAGATGGA GCGGCGGCAG 
CAGCTGGAGC ACGAGGTCCG GAGCGCCCAG GAGGAGATCC GGGCCCTGCG GAGCCAGAGC 
CCGCAGGAGG CGGTGGTGAC CAGGGAGGTG CTCAGGAAGG TGCCGGACCC AGCACTGGAG 
GAGAGCTTCC AGCAGGCGCA GCAGACCCTG GCCGAGGAGC AGCACAAGAA CCGGCTGCTG 
CAGGGGGAGG TGGAGGCGCT GCGGCTGCGG CTGCGTGCCC TGGAGGAGGC GGCCCGGGCC 
GGGGGCCAGG AGTACGTGGT CAAGGAGGTA CTGCGCATTG AGCCGGATCG GGCCCAGGCT 
GACGAGGTCC TGAGGCTGCG GGGGGAGCTG GAGGTGCTGC GGCGGCAGAA GGGCGCCCGC 
GAGGCGGAGG CGCTTCTCCT GCAGCAGCGC GTGGCCGCCC TGGCCGAGGA GAAGCGGCGG 
GCGCGGGAGG TGGTCACAGA GAAGGAGGTG GTCAAGGTGC ACAATGACCC CCAGCTGGAG 
GCCGAGTTCC GGCAGCTGCA GGAGGACCAG CAGCGGGAGC AGGGGCTCCG GGAGAAGCAG 
GAGGAGGAGC TGGGCTTCCT GCAGGACAAG CTCAAGAGGC TGGAGAAGGA ACGGGCCATG 
GCCGAGGGCA AGATCACAGT CAAGGAGGTG CTCAAGGTGG AGAAGGACGT GGCGGCTGAG 
CGGGAGGTGG GCGCCCTCCA GCGCCGGTAT GAGGATGAGG CCGCCAGGGC CCGTGCCAGC 
CAGAGGGAGA AGACCGAGCT GCTCCGGAAG ATCTGGGCCC TGGAGGAGGA GAACGCTAAG 
GTGGTTGTGC AAGAGAAGGT GCGCGAGATT GTCCGGCCTG ACCCCAAGGC TGAGAGTGAG 
GTGGCCAACC TCCGCCTGGA GCTGGTAGAG CAGGAGCGCA AGTACCGGGG GGCTGAGGAG 
CAGCTCAAGA GCTACCAGAG CGAGCTGGAG GCGCTTCGGA GGCGAGGCCC CCAGGTAGAG 
GTCAAGGAGG TGACCAAGGA GGTCATCAAG TACACGACGG ACCCTGAGAC GGAGAAGGAG 
CTCCAGAGGC TCAGGGAGGA GATCGTGGAC AAGACCCGCC TCATCGAGAG GTGTGACCTG 
GAGATCTACC AGCTGAAGCA GGAGATCCAG TCCCTCAAAG AGGCCAAGCC CCAGGTGCAG 
ACCAAGGAGG TGGTCCAGGA GATCTTGCAG TTCCAGGAAG ACCCCCAGAC CAAAGAGGAA 
GTGGCGGCTC TGAGGGCCCG GCTGGCTGAG GAGCACAAGA AGCAGGTAGA CCTGGAACGG 
GAATGCGTGT CACAGGAGGA GAAGATCCAG GAGAAGGAGG AGGAGCTGGC CCGCGGGAAG 
GAGGTGGTGG TGCAGCAGGA GGTGGTCAGG TACGAGGAGG AGCCGGGCCT CCGGGCGGAA 
GTGAGCGCCT TCACCGAGAG CATCGATGCA GAACTGCGGC AGATCGACGG CCTCCGCGGC 
GAGCTGCGGC GGCTGCAGCG CCGGCGAGCC GAGCTGGAGC ACCAGCTGGA GGAGCTGGAG 
CGCGAGCGGC AGGCGCGCCG CGAGGCTGAG CTGGAGGTGC AGCACCTCAA GCAACAGCTG 
GCCCAGCTGG AGGCGCAGGA GGGCGAGGCC CACGAGAAGG TGACCCTCAC ACAGAAGGTG 
GTGCTGCAGC AGGACCCCCA GCAGGCCCGG GAGCATGCCC TCCTGGCCCT GCAGCTGGAG 
GAAGAGCGGC ACCGCCGGCA GGGCCTGGAG CGCGAGCTCG AGACCCTAAA GCAGCAGCTG 
GAGCACCTGG AGAAGATGGA GGTCAAGGAG AAGGTGGTCT TCTCCGAGAG CGTCCAGGTG 
GAGAAAGGCG ACACCGAGCA GGAGATTGAG AGGCTCAGGG GCAGTCTGGA GGCTGAGAGC 
CGAGGCAAGA GGGAGCTGGA CGCCGAGGTG AGCCGGCTGG AGGCCAAGCT GTCGGAGCTG 
GAGTTCTGCA ACTCTAAGTC ATCCAAGGAG CTGGACTTCC TGAGGGAGGA GAACCACAAG 
CTGCAGCTGG AGCGGCAGAG CCTGCAGCTC GAGGCCCGCA GGCTGCAGTC GGAGATCGAG 
ATGGCCAAGG CCGAGGCGCA GAGCCTAAGA AGCCTGCCCA CAACGACTGG AGCCGACCCC 
GGGGCGCAGC TCGACTCACG CCTGCGGTCC CTGGAGCGGG AGCTGGACGA CCTCAGGAGG 
CTCTCCAGGG ACAAAGACCA GGAGATCGAA GAGCTGCAGC GTCGCCTGGG GTCCGTGGCC 
ATCAAGCGGG AGCAGAGGGA GAACCACCTG CGGCGCTCCA TTGTGGTCAT CGACCCTGAC 
TCGGGCCGTG AGCTGTCCCC CGAGGAGGCC CACCGCGCCG GCCTCATCGA CTGGAACATG 
TTTGTCAAGC TCAGGAGCCA GGAGTGCGAC TGGGAGGAGA TCTCCGTCAA GGGCCCCGGA 
GGCGAGTCCT CCGTGATCCA TGACAGGAAG TCCGGCAGGA AGTTCTCCAT TGAAGAGGCC 
CTGCAGAGCG GGCGGCTGAC CCCCGCGCAG TACGACCGCT ACGTCAACAA GAGCATGTCC 
ATCCAGGAGC TGGCCGTCCT GGTGTCAGGG CAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPL ( XM_005224662.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224719.1 Bos taurus periplakin (PPL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa32912
Clone ID Related Accession (Same CDS sequence)	XM_005224662.4 , XM_005224662.3 , XM_010799950.2
Accession Version	XM_005224662.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	periplakin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224662.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCCACGGT TCGGACCCGC 
AGCGTCTCTA ACAAGGAGCT GTCGGAGCTC ATCGAGCAGT TGCAGAAGAA TGCCGACCAG 
GTGGAGAGGA ACATCGTGGA CACGGAAGCC AAGATGCAGA GAGACCTGGC CCGGCTGCAG 
GAGGGCCAGC AGCCCGAGCA CCGGGATGTG GCCCTGCAGA AGGTGTCAGA CTCTGAGAAG 
CTGCTCTACA TGCTGGAGGC GGACGCGGCC ATCGCCAAGC ACATGAAGCA CCCCCAGGGG 
GACATGATCG CCGAGGACAT CCGTCAGCTG AAGGAGCGTG TGACCAACCT CCGCGGGAAA 
CACAAGCAGA TCTACAACCT GGTGGTGAAA GAGGTGGACC CACAGGTCAA CTGGGAGGCG 
CTGGTGGAGG AAAAGCTGGA CGAGCTGAGC AGCCAGGGCT TCGGGACGGA ACTCCCGCTG 
GTAGGCCAGC AGGTGGAGCA ACACAACGTC TTTCACAACG AGGTCAAGGC CATCGGGCCC 
CACCTGGCCA AGGAGCAGAA CGGTGAACTC CAGGCCAAGT ACCAGAAACT GCTGGCGGCG 
TCACAGGCGC GGCAGCAGCA CCTGAGCTCA CTGCAGGACT ACATGCAGCG GTGCACGAAC 
GAGCTGTACT GGCTGGACCA GCAGGCCCAG GGCCGCATAC AGTATGACTG GAGTGACCGC 
AACCTCAACT ACCCCAGCCG CCGGCGCCAG TACGAGAATT TCATCCACCG GAACCTGGAG 
GCCAAAGAGG AGAGAATTAA CAAGCTGCAC AGCGAGGGTG ACCAGCTGCT CGCCGCCGAG 
CACCCCGGGA GGAACTCCAT TGAAGCTCAC ATGGAGGCTG TCCACGCAGA TTGGAAGGAA 
TACCTGAACC TGCTCATCTG CGAGGAGAGC CACCTGAAGT ACATGGAAGA CTACCACCAG 
TTCCACAAAG ACATGAAGGA TGCCCAGGAG CTGCTTCGCA AGGTGGACTC AGACCTGAAC 
CAGAAATACA GCCCTGACTT CAAGGACCGA TACCAGATTG AGCTGCTGCT GCGGGAGCTG 
GATGACCAGG AGAAGGCTCT GGACAAGTAC CAGCATGTGG TGCGGGGGCT GCAGAAGCGA 
GGGCAGCAGG TGGTGCCCCT CAAGTACCGC CGGGAGACGC CCCTCAAGCC CATCCCCGTG 
GAGGCGCTCT GTGACTTCGA GGGTGACCAG GGCCTGATCT CTCGGGGCTA CAGCTACACC 
CTGCAGAGGA ACAATGGGGA GAGCTGGGAC CTCACCGACA GTGCGGGGAA CAAGCTGACC 
GCCCCGGCCG TCTGCTTCAT GATCCCGCCC ACTGACCCCG AGGCCCTGGC TCTGGCTGAA 
AGCCTGGGCA GCCAGTACCA GAGCCTGCGG CAGAAGGCAG CTGGGAGCAG AAAGGCGCTC 
CAGGAGCAGC ATGAGGTGCT GAAGGCAGAG AGCTCTGGAG CAGATGCCTC GGACCTGCAG 
GGCCGCCAGC TGCTGGCCGG CTTGGACAAG GTGGCCAGCG ACCTGGACCG GCAGGAGAAG 
GCCATCACGG CCATCCTGCG GCCGCCGCTG GAGCAGGGCC GGGCTGTGCA GGACAGCGCC 
GAGAGGGCCA AGGACCTCAA GAACATCACC AACGAGCTGC TGCGGATCGA GCCCGAGAAG 
GCGCGCAGCA CGGCCGAGGG CGAGGCGTTC GTGCGGGCCC TCCCGGACAG CGGCAGCACC 
GCCCTGCTGA GGACGCGGGT GGAGGACACC CAGCGCAGAT ACGAGCGCCT GGTGCAGCTG 
CTGGAAGCCG CCCAGGAGAA GCTCGACATC GCCAACCGCC TGGAGAAGAG CCTGCAGCAG 
GGCCGGGAGA TGCTGGCCAC GTATGAGACC CAGCTGGCCC AGGAGGACAC GGTGCCCGAG 
AGTGGCCAGG CCCTGGACAG CAGGAGGCAG GAGCTGGCGG CCATGGCCTC CGAGCTGCAG 
GCCCGGAAGG CCCTCCTCGG CGAGGTGCAG CGGAACCTGC AGGCCGCCAA GCAGTGCTCG 
GGCTCGCTGG CCAGCCGCTT CCAGGAGCAC TGCCCGGACC TGGAGCGCCA GGAGGCCGAG 
GTGCACAAGC TGTGCCAGCG CTTCGACGGC CTCTGCCAGC AGGCGGAGCT CAGGGTCCAG 
AGCCTGCAGA GCGCCCGGGC TGAGCACGAC GCCTTCTGCA GCGGCCGTGA CCGCCTGCTT 
CAGTTCCTGT CCCACATCCC CAGCTACGAG CCCCAGGAGA CGGACGGCCT CGGCCAAGTG 
GAGACCAAGC TGAACAACCA GAAGAACCTA CTAGATGAAA TAGCAAGAAG GGAAGAGGAA 
GTGCAGAAAG TCCACACGCA CTCCCAGCAG TACCAGCAGG CTGTCAAGGA CTACGAGTTG 
GAAGCAGAGA AACTCAGGTC CCTCCTTGAC TTGGAGAATG GAAGGAGCAG CCACGTGAGC 
AAGCGGGCCA GGCTCCAGTC CCCTGCCACC AAAGTGAGGG AAGAGGAAGC AGCTCTTGCT 
GCCAAGTTTA CGGAGGTTAA TGCCATCAAC AGACAGAGAC TGCAGAACCT GGAGTTTGCA 
CTGACCCTCC TGAGACAGCA GCTGGAGGCA GGTGCAAGCC AGGAGGCCCT GCCCGGGAGC 
AAGCCAGGCC CTGGAGCAGA GGAGACGTGG AAGATTCAGG AGGAGCTGGA TGAGGAGATG 
GAGCGGCGGC AGCAGCTGGA GCACGAGGTC CGGAGCGCCC AGGAGGAGAT CCGGGCCCTG 
CGGAGCCAGA GCCCGCAGGA GGCGGTGGTG ACCAGGGAGG TGCTCAGGAA GGTGCCGGAC 
CCAGCACTGG AGGAGAGCTT CCAGCAGGCG CAGCAGACCC TGGCCGAGGA GCAGCACAAG 
AACCGGCTGC TGCAGGGGGA GGTGGAGGCG CTGCGGCTGC GGCTGCGTGC CCTGGAGGAG 
GCGGCCCGGG CCGGGGGCCA GGAGTACGTG GTCAAGGAGG TACTGCGCAT TGAGCCGGAT 
CGGGCCCAGG CTGACGAGGT CCTGAGGCTG CGGGGGGAGC TGGAGGTGCT GCGGCGGCAG 
AAGGGCGCCC GCGAGGCGGA GGCGCTTCTC CTGCAGCAGC GCGTGGCCGC CCTGGCCGAG 
GAGAAGCGGC GGGCGCGGGA GGTGGTCACA GAGAAGGAGG TGGTCAAGGT GCACAATGAC 
CCCCAGCTGG AGGCCGAGTT CCGGCAGCTG CAGGAGGACC AGCAGCGGGA GCAGGGGCTC 
CGGGAGAAGC AGGAGGAGGA GCTGGGCTTC CTGCAGGACA AGCTCAAGAG GCTGGAGAAG 
GAACGGGCCA TGGCCGAGGG CAAGATCACA GTCAAGGAGG TGCTCAAGGT GGAGAAGGAC 
GTGGCGGCTG AGCGGGAGGT GGGCGCCCTC CAGCGCCGGT ATGAGGATGA GGCCGCCAGG 
GCCCGTGCCA GCCAGAGGGA GAAGACCGAG CTGCTCCGGA AGATCTGGGC CCTGGAGGAG 
GAGAACGCTA AGGTGGTTGT GCAAGAGAAG GTGCGCGAGA TTGTCCGGCC TGACCCCAAG 
GCTGAGAGTG AGGTGGCCAA CCTCCGCCTG GAGCTGGTAG AGCAGGAGCG CAAGTACCGG 
GGGGCTGAGG AGCAGCTCAA GAGCTACCAG AGCGAGCTGG AGGCGCTTCG GAGGCGAGGC 
CCCCAGGTAG AGGTCAAGGA GGTGACCAAG GAGGTCATCA AGTACACGAC GGACCCTGAG 
ACGGAGAAGG AGCTCCAGAG GCTCAGGGAG GAGATCGTGG ACAAGACCCG CCTCATCGAG 
AGGTGTGACC TGGAGATCTA CCAGCTGAAG CAGGAGATCC AGTCCCTCAA AGAGGCCAAG 
CCCCAGGTGC AGACCAAGGA GGTGGTCCAG GAGATCTTGC AGTTCCAGGA AGACCCCCAG 
ACCAAAGAGG AAGTGGCGGC TCTGAGGGCC CGGCTGGCTG AGGAGCACAA GAAGCAGGTA 
GACCTGGAAC GGGAATGCGT GTCACAGGAG GAGAAGATCC AGGAGAAGGA GGAGGAGCTG 
GCCCGCGGGA AGGAGGTGGT GGTGCAGCAG GAGGTGGTCA GGTACGAGGA GGAGCCGGGC 
CTCCGGGCGG AAGTGAGCGC CTTCACCGAG AGCATCGATG CAGAACTGCG GCAGATCGAC 
GGCCTCCGCG GCGAGCTGCG GCGGCTGCAG CGCCGGCGAG CCGAGCTGGA GCACCAGCTG 
GAGGAGCTGG AGCGCGAGCG GCAGGCGCGC CGCGAGGCTG AGCTGGAGGT GCAGCACCTC 
AAGCAACAGC TGGCCCAGCT GGAGGCGCAG GAGGGCGAGG CCCACGAGAA GGTGACCCTC 
ACACAGAAGG TGGTGCTGCA GCAGGACCCC CAGCAGGCCC GGGAGCATGC CCTCCTGGCC 
CTGCAGCTGG AGGAAGAGCG GCACCGCCGG CAGGGCCTGG AGCGCGAGCT CGAGACCCTA 
AAGCAGCAGC TGGAGCACCT GGAGAAGATG GAGGTCAAGG AGAAGGTGGT CTTCTCCGAG 
AGCGTCCAGG TGGAGAAAGG CGACACCGAG CAGGAGATTG AGAGGCTCAG GGGCAGTCTG 
GAGGCTGAGA GCCGAGGCAA GAGGGAGCTG GACGCCGAGG TGAGCCGGCT GGAGGCCAAG 
CTGTCGGAGC TGGAGTTCTG CAACTCTAAG TCATCCAAGG AGCTGGACTT CCTGAGGGAG 
GAGAACCACA AGCTGCAGCT GGAGCGGCAG AGCCTGCAGC TCGAGGCCCG CAGGCTGCAG 
TCGGAGATCG AGATGGCCAA GGCCGAGGCG CAGAGCCTAA GAAGCCTGCC CACAACGACT 
GGAGCCGACC CCGGGGCGCA GCTCGACTCA CGCCTGCGGT CCCTGGAGCG GGAGCTGGAC 
GACCTCAGGA GGCTCTCCAG GGACAAAGAC CAGGAGATCG AAGAGCTGCA GCGTCGCCTG 
GGGTCCGTGG CCATCAAGCG GGAGCAGAGG GAGAACCACC TGCGGCGCTC CATTGTGGTC 
ATCGACCCTG ACTCGGGCCG TGAGCTGTCC CCCGAGGAGG CCCACCGCGC CGGCCTCATC 
GACTGGAACA TGTTTGTCAA GCTCAGGAGC CAGGAGTGCG ACTGGGAGGA GATCTCCGTC 
AAGGGCCCCG GAGGCGAGTC CTCCGTGATC CATGACAGGA AGTCCGGCAG GAAGTTCTCC 
ATTGAAGAGG CCCTGCAGAG CGGGCGGCTG ACCCCCGCGC AGTACGACCG CTACGTCAAC 
AAGAGCATGT CCATCCAGGA GCTGGCCGTC CTGGTGTCAG GGCAGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224719.1
CDS	225..5495
Translation	MNSLFRKRNKGKYSPTVRTRSVSNKELSELIEQLQKNADQVERNIVDTEAKMQRDLARLQEGQQPEHRDVALQKVSDSEKLLYMLEADAAIAKHMKHPQGDMIAEDIRQLKERVTNLRGKHKQIYNLVVKEVDPQVNWEALVEEKLDELSSQGFGTELPLVGQQVEQHNVFHNEVKAIGPHLAKEQNGELQAKYQKLLAASQARQQHLSSLQDYMQRCTNELYWLDQQAQGRIQYDWSDRNLNYPSRRRQYENFIHRNLEAKEERINKLHSEGDQLLAAEHPGRNSIEAHMEAVHADWKEYLNLLICEESHLKYMEDYHQFHKDMKDAQELLRKVDSDLNQKYSPDFKDRYQIELLLRELDDQEKALDKYQHVVRGLQKRGQQVVPLKYRRETPLKPIPVEALCDFEGDQGLISRGYSYTLQRNNGESWDLTDSAGNKLTAPAVCFMIPPTDPEALALAESLGSQYQSLRQKAAGSRKALQEQHEVLKAESSGADASDLQGRQLLAGLDKVASDLDRQEKAITAILRPPLEQGRAVQDSAERAKDLKNITNELLRIEPEKARSTAEGEAFVRALPDSGSTALLRTRVEDTQRRYERLVQLLEAAQEKLDIANRLEKSLQQGREMLATYETQLAQEDTVPESGQALDSRRQELAAMASELQARKALLGEVQRNLQAAKQCSGSLASRFQEHCPDLERQEAEVHKLCQRFDGLCQQAELRVQSLQSARAEHDAFCSGRDRLLQFLSHIPSYEPQETDGLGQVETKLNNQKNLLDEIARREEEVQKVHTHSQQYQQAVKDYELEAEKLRSLLDLENGRSSHVSKRARLQSPATKVREEEAALAAKFTEVNAINRQRLQNLEFALTLLRQQLEAGASQEALPGSKPGPGAEETWKIQEELDEEMERRQQLEHEVRSAQEEIRALRSQSPQEAVVTREVLRKVPDPALEESFQQAQQTLAEEQHKNRLLQGEVEALRLRLRALEEAARAGGQEYVVKEVLRIEPDRAQADEVLRLRGELEVLRRQKGAREAEALLLQQRVAALAEEKRRAREVVTEKEVVKVHNDPQLEAEFRQLQEDQQREQGLREKQEEELGFLQDKLKRLEKERAMAEGKITVKEVLKVEKDVAAEREVGALQRRYEDEAARARASQREKTELLRKIWALEEENAKVVVQEKVREIVRPDPKAESEVANLRLELVEQERKYRGAEEQLKSYQSELEALRRRGPQVEVKEVTKEVIKYTTDPETEKELQRLREEIVDKTRLIERCDLEIYQLKQEIQSLKEAKPQVQTKEVVQEILQFQEDPQTKEEVAALRARLAEEHKKQVDLERECVSQEEKIQEKEEELARGKEVVVQQEVVRYEEEPGLRAEVSAFTESIDAELRQIDGLRGELRRLQRRRAELEHQLEELERERQARREAELEVQHLKQQLAQLEAQEGEAHEKVTLTQKVVLQQDPQQAREHALLALQLEEERHRRQGLERELETLKQQLEHLEKMEVKEKVVFSESVQVEKGDTEQEIERLRGSLEAESRGKRELDAEVSRLEAKLSELEFCNSKSSKELDFLREENHKLQLERQSLQLEARRLQSEIEMAKAEAQSLRSLPTTTGADPGAQLDSRLRSLERELDDLRRLSRDKDQEIEELQRRLGSVAIKREQRENHLRRSIVVIDPDSGRELSPEEAHRAGLIDWNMFVKLRSQECDWEEISVKGPGGESSVIHDRKSGRKFSIEEALQSGRLTPAQYDRYVNKSMSIQELAVLVSGQK

Target ORF information:

RefSeq Version	XM_005224662.4
Organism	Bos taurus(cattle)
Definition	Bos taurus periplakin (PPL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224662.4

ORF Insert Sequence:

ATGAACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCCACGGT TCGGACCCGC 
AGCGTCTCTA ACAAGGAGCT GTCGGAGCTC ATCGAGCAGT TGCAGAAGAA TGCCGACCAG 
GTGGAGAGGA ACATCGTGGA CACGGAAGCC AAGATGCAGA GAGACCTGGC CCGGCTGCAG 
GAGGGCCAGC AGCCCGAGCA CCGGGATGTG GCCCTGCAGA AGGTGTCAGA CTCTGAGAAG 
CTGCTCTACA TGCTGGAGGC GGACGCGGCC ATCGCCAAGC ACATGAAGCA CCCCCAGGGG 
GACATGATCG CCGAGGACAT CCGTCAGCTG AAGGAGCGTG TGACCAACCT CCGCGGGAAA 
CACAAGCAGA TCTACAACCT GGTGGTGAAA GAGGTGGACC CACAGGTCAA CTGGGAGGCG 
CTGGTGGAGG AAAAGCTGGA CGAGCTGAGC AGCCAGGGCT TCGGGACGGA ACTCCCGCTG 
GTAGGCCAGC AGGTGGAGCA ACACAACGTC TTTCACAACG AGGTCAAGGC CATCGGGCCC 
CACCTGGCCA AGGAGCAGAA CGGTGAACTC CAGGCCAAGT ACCAGAAACT GCTGGCGGCG 
TCACAGGCGC GGCAGCAGCA CCTGAGCTCA CTGCAGGACT ACATGCAGCG GTGCACGAAC 
GAGCTGTACT GGCTGGACCA GCAGGCCCAG GGCCGCATAC AGTATGACTG GAGTGACCGC 
AACCTCAACT ACCCCAGCCG CCGGCGCCAG TACGAGAATT TCATCCACCG GAACCTGGAG 
GCCAAAGAGG AGAGAATTAA CAAGCTGCAC AGCGAGGGTG ACCAGCTGCT CGCCGCCGAG 
CACCCCGGGA GGAACTCCAT TGAAGCTCAC ATGGAGGCTG TCCACGCAGA TTGGAAGGAA 
TACCTGAACC TGCTCATCTG CGAGGAGAGC CACCTGAAGT ACATGGAAGA CTACCACCAG 
TTCCACAAAG ACATGAAGGA TGCCCAGGAG CTGCTTCGCA AGGTGGACTC AGACCTGAAC 
CAGAAATACA GCCCTGACTT CAAGGACCGA TACCAGATTG AGCTGCTGCT GCGGGAGCTG 
GATGACCAGG AGAAGGCTCT GGACAAGTAC CAGCATGTGG TGCGGGGGCT GCAGAAGCGA 
GGGCAGCAGG TGGTGCCCCT CAAGTACCGC CGGGAGACGC CCCTCAAGCC CATCCCCGTG 
GAGGCGCTCT GTGACTTCGA GGGTGACCAG GGCCTGATCT CTCGGGGCTA CAGCTACACC 
CTGCAGAGGA ACAATGGGGA GAGCTGGGAC CTCACCGACA GTGCGGGGAA CAAGCTGACC 
GCCCCGGCCG TCTGCTTCAT GATCCCGCCC ACTGACCCCG AGGCCCTGGC TCTGGCTGAA 
AGCCTGGGCA GCCAGTACCA GAGCCTGCGG CAGAAGGCAG CTGGGAGCAG AAAGGCGCTC 
CAGGAGCAGC ATGAGGTGCT GAAGGCAGAG AGCTCTGGAG CAGATGCCTC GGACCTGCAG 
GGCCGCCAGC TGCTGGCCGG CTTGGACAAG GTGGCCAGCG ACCTGGACCG GCAGGAGAAG 
GCCATCACGG CCATCCTGCG GCCGCCGCTG GAGCAGGGCC GGGCTGTGCA GGACAGCGCC 
GAGAGGGCCA AGGACCTCAA GAACATCACC AACGAGCTGC TGCGGATCGA GCCCGAGAAG 
GCGCGCAGCA CGGCCGAGGG CGAGGCGTTC GTGCGGGCCC TCCCGGACAG CGGCAGCACC 
GCCCTGCTGA GGACGCGGGT GGAGGACACC CAGCGCAGAT ACGAGCGCCT GGTGCAGCTG 
CTGGAAGCCG CCCAGGAGAA GCTCGACATC GCCAACCGCC TGGAGAAGAG CCTGCAGCAG 
GGCCGGGAGA TGCTGGCCAC GTATGAGACC CAGCTGGCCC AGGAGGACAC GGTGCCCGAG 
AGTGGCCAGG CCCTGGACAG CAGGAGGCAG GAGCTGGCGG CCATGGCCTC CGAGCTGCAG 
GCCCGGAAGG CCCTCCTCGG CGAGGTGCAG CGGAACCTGC AGGCCGCCAA GCAGTGCTCG 
GGCTCGCTGG CCAGCCGCTT CCAGGAGCAC TGCCCGGACC TGGAGCGCCA GGAGGCCGAG 
GTGCACAAGC TGTGCCAGCG CTTCGACGGC CTCTGCCAGC AGGCGGAGCT CAGGGTCCAG 
AGCCTGCAGA GCGCCCGGGC TGAGCACGAC GCCTTCTGCA GCGGCCGTGA CCGCCTGCTT 
CAGTTCCTGT CCCACATCCC CAGCTACGAG CCCCAGGAGA CGGACGGCCT CGGCCAAGTG 
GAGACCAAGC TGAACAACCA GAAGAACCTA CTAGATGAAA TAGCAAGAAG GGAAGAGGAA 
GTGCAGAAAG TCCACACGCA CTCCCAGCAG TACCAGCAGG CTGTCAAGGA CTACGAGTTG 
GAAGCAGAGA AACTCAGGTC CCTCCTTGAC TTGGAGAATG GAAGGAGCAG CCACGTGAGC 
AAGCGGGCCA GGCTCCAGTC CCCTGCCACC AAAGTGAGGG AAGAGGAAGC AGCTCTTGCT 
GCCAAGTTTA CGGAGGTTAA TGCCATCAAC AGACAGAGAC TGCAGAACCT GGAGTTTGCA 
CTGACCCTCC TGAGACAGCA GCTGGAGGCA GGTGCAAGCC AGGAGGCCCT GCCCGGGAGC 
AAGCCAGGCC CTGGAGCAGA GGAGACGTGG AAGATTCAGG AGGAGCTGGA TGAGGAGATG 
GAGCGGCGGC AGCAGCTGGA GCACGAGGTC CGGAGCGCCC AGGAGGAGAT CCGGGCCCTG 
CGGAGCCAGA GCCCGCAGGA GGCGGTGGTG ACCAGGGAGG TGCTCAGGAA GGTGCCGGAC 
CCAGCACTGG AGGAGAGCTT CCAGCAGGCG CAGCAGACCC TGGCCGAGGA GCAGCACAAG 
AACCGGCTGC TGCAGGGGGA GGTGGAGGCG CTGCGGCTGC GGCTGCGTGC CCTGGAGGAG 
GCGGCCCGGG CCGGGGGCCA GGAGTACGTG GTCAAGGAGG TACTGCGCAT TGAGCCGGAT 
CGGGCCCAGG CTGACGAGGT CCTGAGGCTG CGGGGGGAGC TGGAGGTGCT GCGGCGGCAG 
AAGGGCGCCC GCGAGGCGGA GGCGCTTCTC CTGCAGCAGC GCGTGGCCGC CCTGGCCGAG 
GAGAAGCGGC GGGCGCGGGA GGTGGTCACA GAGAAGGAGG TGGTCAAGGT GCACAATGAC 
CCCCAGCTGG AGGCCGAGTT CCGGCAGCTG CAGGAGGACC AGCAGCGGGA GCAGGGGCTC 
CGGGAGAAGC AGGAGGAGGA GCTGGGCTTC CTGCAGGACA AGCTCAAGAG GCTGGAGAAG 
GAACGGGCCA TGGCCGAGGG CAAGATCACA GTCAAGGAGG TGCTCAAGGT GGAGAAGGAC 
GTGGCGGCTG AGCGGGAGGT GGGCGCCCTC CAGCGCCGGT ATGAGGATGA GGCCGCCAGG 
GCCCGTGCCA GCCAGAGGGA GAAGACCGAG CTGCTCCGGA AGATCTGGGC CCTGGAGGAG 
GAGAACGCTA AGGTGGTTGT GCAAGAGAAG GTGCGCGAGA TTGTCCGGCC TGACCCCAAG 
GCTGAGAGTG AGGTGGCCAA CCTCCGCCTG GAGCTGGTAG AGCAGGAGCG CAAGTACCGG 
GGGGCTGAGG AGCAGCTCAA GAGCTACCAG AGCGAGCTGG AGGCGCTTCG GAGGCGAGGC 
CCCCAGGTAG AGGTCAAGGA GGTGACCAAG GAGGTCATCA AGTACACGAC GGACCCTGAG 
ACGGAGAAGG AGCTCCAGAG GCTCAGGGAG GAGATCGTGG ACAAGACCCG CCTCATCGAG 
AGGTGTGACC TGGAGATCTA CCAGCTGAAG CAGGAGATCC AGTCCCTCAA AGAGGCCAAG 
CCCCAGGTGC AGACCAAGGA GGTGGTCCAG GAGATCTTGC AGTTCCAGGA AGACCCCCAG 
ACCAAAGAGG AAGTGGCGGC TCTGAGGGCC CGGCTGGCTG AGGAGCACAA GAAGCAGGTA 
GACCTGGAAC GGGAATGCGT GTCACAGGAG GAGAAGATCC AGGAGAAGGA GGAGGAGCTG 
GCCCGCGGGA AGGAGGTGGT GGTGCAGCAG GAGGTGGTCA GGTACGAGGA GGAGCCGGGC 
CTCCGGGCGG AAGTGAGCGC CTTCACCGAG AGCATCGATG CAGAACTGCG GCAGATCGAC 
GGCCTCCGCG GCGAGCTGCG GCGGCTGCAG CGCCGGCGAG CCGAGCTGGA GCACCAGCTG 
GAGGAGCTGG AGCGCGAGCG GCAGGCGCGC CGCGAGGCTG AGCTGGAGGT GCAGCACCTC 
AAGCAACAGC TGGCCCAGCT GGAGGCGCAG GAGGGCGAGG CCCACGAGAA GGTGACCCTC 
ACACAGAAGG TGGTGCTGCA GCAGGACCCC CAGCAGGCCC GGGAGCATGC CCTCCTGGCC 
CTGCAGCTGG AGGAAGAGCG GCACCGCCGG CAGGGCCTGG AGCGCGAGCT CGAGACCCTA 
AAGCAGCAGC TGGAGCACCT GGAGAAGATG GAGGTCAAGG AGAAGGTGGT CTTCTCCGAG 
AGCGTCCAGG TGGAGAAAGG CGACACCGAG CAGGAGATTG AGAGGCTCAG GGGCAGTCTG 
GAGGCTGAGA GCCGAGGCAA GAGGGAGCTG GACGCCGAGG TGAGCCGGCT GGAGGCCAAG 
CTGTCGGAGC TGGAGTTCTG CAACTCTAAG TCATCCAAGG AGCTGGACTT CCTGAGGGAG 
GAGAACCACA AGCTGCAGCT GGAGCGGCAG AGCCTGCAGC TCGAGGCCCG CAGGCTGCAG 
TCGGAGATCG AGATGGCCAA GGCCGAGGCG CAGAGCCTAA GAAGCCTGCC CACAACGACT 
GGAGCCGACC CCGGGGCGCA GCTCGACTCA CGCCTGCGGT CCCTGGAGCG GGAGCTGGAC 
GACCTCAGGA GGCTCTCCAG GGACAAAGAC CAGGAGATCG AAGAGCTGCA GCGTCGCCTG 
GGGTCCGTGG CCATCAAGCG GGAGCAGAGG GAGAACCACC TGCGGCGCTC CATTGTGGTC 
ATCGACCCTG ACTCGGGCCG TGAGCTGTCC CCCGAGGAGG CCCACCGCGC CGGCCTCATC 
GACTGGAACA TGTTTGTCAA GCTCAGGAGC CAGGAGTGCG ACTGGGAGGA GATCTCCGTC 
AAGGGCCCCG GAGGCGAGTC CTCCGTGATC CATGACAGGA AGTCCGGCAG GAAGTTCTCC 
ATTGAAGAGG CCCTGCAGAG CGGGCGGCTG ACCCCCGCGC AGTACGACCG CTACGTCAAC 
AAGAGCATGT CCATCCAGGA GCTGGCCGTC CTGGTGTCAG GGCAGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPL ( XM_005224662.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224719.1 Bos taurus periplakin (PPL), transcript variant X1, mRNA.

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CloneID	OBa32912
Clone ID Related Accession (Same CDS sequence)	XM_005224662.4 , XM_005224662.3 , XM_010799950.2
Accession Version	XM_005224662.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	periplakin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224662.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCCACGGT TCGGACCCGC 
AGCGTCTCTA ACAAGGAGCT GTCGGAGCTC ATCGAGCAGT TGCAGAAGAA TGCCGACCAG 
GTGGAGAGGA ACATCGTGGA CACGGAAGCC AAGATGCAGA GAGACCTGGC CCGGCTGCAG 
GAGGGCCAGC AGCCCGAGCA CCGGGATGTG GCCCTGCAGA AGGTGTCAGA CTCTGAGAAG 
CTGCTCTACA TGCTGGAGGC GGACGCGGCC ATCGCCAAGC ACATGAAGCA CCCCCAGGGG 
GACATGATCG CCGAGGACAT CCGTCAGCTG AAGGAGCGTG TGACCAACCT CCGCGGGAAA 
CACAAGCAGA TCTACAACCT GGTGGTGAAA GAGGTGGACC CACAGGTCAA CTGGGAGGCG 
CTGGTGGAGG AAAAGCTGGA CGAGCTGAGC AGCCAGGGCT TCGGGACGGA ACTCCCGCTG 
GTAGGCCAGC AGGTGGAGCA ACACAACGTC TTTCACAACG AGGTCAAGGC CATCGGGCCC 
CACCTGGCCA AGGAGCAGAA CGGTGAACTC CAGGCCAAGT ACCAGAAACT GCTGGCGGCG 
TCACAGGCGC GGCAGCAGCA CCTGAGCTCA CTGCAGGACT ACATGCAGCG GTGCACGAAC 
GAGCTGTACT GGCTGGACCA GCAGGCCCAG GGCCGCATAC AGTATGACTG GAGTGACCGC 
AACCTCAACT ACCCCAGCCG CCGGCGCCAG TACGAGAATT TCATCCACCG GAACCTGGAG 
GCCAAAGAGG AGAGAATTAA CAAGCTGCAC AGCGAGGGTG ACCAGCTGCT CGCCGCCGAG 
CACCCCGGGA GGAACTCCAT TGAAGCTCAC ATGGAGGCTG TCCACGCAGA TTGGAAGGAA 
TACCTGAACC TGCTCATCTG CGAGGAGAGC CACCTGAAGT ACATGGAAGA CTACCACCAG 
TTCCACAAAG ACATGAAGGA TGCCCAGGAG CTGCTTCGCA AGGTGGACTC AGACCTGAAC 
CAGAAATACA GCCCTGACTT CAAGGACCGA TACCAGATTG AGCTGCTGCT GCGGGAGCTG 
GATGACCAGG AGAAGGCTCT GGACAAGTAC CAGCATGTGG TGCGGGGGCT GCAGAAGCGA 
GGGCAGCAGG TGGTGCCCCT CAAGTACCGC CGGGAGACGC CCCTCAAGCC CATCCCCGTG 
GAGGCGCTCT GTGACTTCGA GGGTGACCAG GGCCTGATCT CTCGGGGCTA CAGCTACACC 
CTGCAGAGGA ACAATGGGGA GAGCTGGGAC CTCACCGACA GTGCGGGGAA CAAGCTGACC 
GCCCCGGCCG TCTGCTTCAT GATCCCGCCC ACTGACCCCG AGGCCCTGGC TCTGGCTGAA 
AGCCTGGGCA GCCAGTACCA GAGCCTGCGG CAGAAGGCAG CTGGGAGCAG AAAGGCGCTC 
CAGGAGCAGC ATGAGGTGCT GAAGGCAGAG AGCTCTGGAG CAGATGCCTC GGACCTGCAG 
GGCCGCCAGC TGCTGGCCGG CTTGGACAAG GTGGCCAGCG ACCTGGACCG GCAGGAGAAG 
GCCATCACGG CCATCCTGCG GCCGCCGCTG GAGCAGGGCC GGGCTGTGCA GGACAGCGCC 
GAGAGGGCCA AGGACCTCAA GAACATCACC AACGAGCTGC TGCGGATCGA GCCCGAGAAG 
GCGCGCAGCA CGGCCGAGGG CGAGGCGTTC GTGCGGGCCC TCCCGGACAG CGGCAGCACC 
GCCCTGCTGA GGACGCGGGT GGAGGACACC CAGCGCAGAT ACGAGCGCCT GGTGCAGCTG 
CTGGAAGCCG CCCAGGAGAA GCTCGACATC GCCAACCGCC TGGAGAAGAG CCTGCAGCAG 
GGCCGGGAGA TGCTGGCCAC GTATGAGACC CAGCTGGCCC AGGAGGACAC GGTGCCCGAG 
AGTGGCCAGG CCCTGGACAG CAGGAGGCAG GAGCTGGCGG CCATGGCCTC CGAGCTGCAG 
GCCCGGAAGG CCCTCCTCGG CGAGGTGCAG CGGAACCTGC AGGCCGCCAA GCAGTGCTCG 
GGCTCGCTGG CCAGCCGCTT CCAGGAGCAC TGCCCGGACC TGGAGCGCCA GGAGGCCGAG 
GTGCACAAGC TGTGCCAGCG CTTCGACGGC CTCTGCCAGC AGGCGGAGCT CAGGGTCCAG 
AGCCTGCAGA GCGCCCGGGC TGAGCACGAC GCCTTCTGCA GCGGCCGTGA CCGCCTGCTT 
CAGTTCCTGT CCCACATCCC CAGCTACGAG CCCCAGGAGA CGGACGGCCT CGGCCAAGTG 
GAGACCAAGC TGAACAACCA GAAGAACCTA CTAGATGAAA TAGCAAGAAG GGAAGAGGAA 
GTGCAGAAAG TCCACACGCA CTCCCAGCAG TACCAGCAGG CTGTCAAGGA CTACGAGTTG 
GAAGCAGAGA AACTCAGGTC CCTCCTTGAC TTGGAGAATG GAAGGAGCAG CCACGTGAGC 
AAGCGGGCCA GGCTCCAGTC CCCTGCCACC AAAGTGAGGG AAGAGGAAGC AGCTCTTGCT 
GCCAAGTTTA CGGAGGTTAA TGCCATCAAC AGACAGAGAC TGCAGAACCT GGAGTTTGCA 
CTGACCCTCC TGAGACAGCA GCTGGAGGCA GGTGCAAGCC AGGAGGCCCT GCCCGGGAGC 
AAGCCAGGCC CTGGAGCAGA GGAGACGTGG AAGATTCAGG AGGAGCTGGA TGAGGAGATG 
GAGCGGCGGC AGCAGCTGGA GCACGAGGTC CGGAGCGCCC AGGAGGAGAT CCGGGCCCTG 
CGGAGCCAGA GCCCGCAGGA GGCGGTGGTG ACCAGGGAGG TGCTCAGGAA GGTGCCGGAC 
CCAGCACTGG AGGAGAGCTT CCAGCAGGCG CAGCAGACCC TGGCCGAGGA GCAGCACAAG 
AACCGGCTGC TGCAGGGGGA GGTGGAGGCG CTGCGGCTGC GGCTGCGTGC CCTGGAGGAG 
GCGGCCCGGG CCGGGGGCCA GGAGTACGTG GTCAAGGAGG TACTGCGCAT TGAGCCGGAT 
CGGGCCCAGG CTGACGAGGT CCTGAGGCTG CGGGGGGAGC TGGAGGTGCT GCGGCGGCAG 
AAGGGCGCCC GCGAGGCGGA GGCGCTTCTC CTGCAGCAGC GCGTGGCCGC CCTGGCCGAG 
GAGAAGCGGC GGGCGCGGGA GGTGGTCACA GAGAAGGAGG TGGTCAAGGT GCACAATGAC 
CCCCAGCTGG AGGCCGAGTT CCGGCAGCTG CAGGAGGACC AGCAGCGGGA GCAGGGGCTC 
CGGGAGAAGC AGGAGGAGGA GCTGGGCTTC CTGCAGGACA AGCTCAAGAG GCTGGAGAAG 
GAACGGGCCA TGGCCGAGGG CAAGATCACA GTCAAGGAGG TGCTCAAGGT GGAGAAGGAC 
GTGGCGGCTG AGCGGGAGGT GGGCGCCCTC CAGCGCCGGT ATGAGGATGA GGCCGCCAGG 
GCCCGTGCCA GCCAGAGGGA GAAGACCGAG CTGCTCCGGA AGATCTGGGC CCTGGAGGAG 
GAGAACGCTA AGGTGGTTGT GCAAGAGAAG GTGCGCGAGA TTGTCCGGCC TGACCCCAAG 
GCTGAGAGTG AGGTGGCCAA CCTCCGCCTG GAGCTGGTAG AGCAGGAGCG CAAGTACCGG 
GGGGCTGAGG AGCAGCTCAA GAGCTACCAG AGCGAGCTGG AGGCGCTTCG GAGGCGAGGC 
CCCCAGGTAG AGGTCAAGGA GGTGACCAAG GAGGTCATCA AGTACACGAC GGACCCTGAG 
ACGGAGAAGG AGCTCCAGAG GCTCAGGGAG GAGATCGTGG ACAAGACCCG CCTCATCGAG 
AGGTGTGACC TGGAGATCTA CCAGCTGAAG CAGGAGATCC AGTCCCTCAA AGAGGCCAAG 
CCCCAGGTGC AGACCAAGGA GGTGGTCCAG GAGATCTTGC AGTTCCAGGA AGACCCCCAG 
ACCAAAGAGG AAGTGGCGGC TCTGAGGGCC CGGCTGGCTG AGGAGCACAA GAAGCAGGTA 
GACCTGGAAC GGGAATGCGT GTCACAGGAG GAGAAGATCC AGGAGAAGGA GGAGGAGCTG 
GCCCGCGGGA AGGAGGTGGT GGTGCAGCAG GAGGTGGTCA GGTACGAGGA GGAGCCGGGC 
CTCCGGGCGG AAGTGAGCGC CTTCACCGAG AGCATCGATG CAGAACTGCG GCAGATCGAC 
GGCCTCCGCG GCGAGCTGCG GCGGCTGCAG CGCCGGCGAG CCGAGCTGGA GCACCAGCTG 
GAGGAGCTGG AGCGCGAGCG GCAGGCGCGC CGCGAGGCTG AGCTGGAGGT GCAGCACCTC 
AAGCAACAGC TGGCCCAGCT GGAGGCGCAG GAGGGCGAGG CCCACGAGAA GGTGACCCTC 
ACACAGAAGG TGGTGCTGCA GCAGGACCCC CAGCAGGCCC GGGAGCATGC CCTCCTGGCC 
CTGCAGCTGG AGGAAGAGCG GCACCGCCGG CAGGGCCTGG AGCGCGAGCT CGAGACCCTA 
AAGCAGCAGC TGGAGCACCT GGAGAAGATG GAGGTCAAGG AGAAGGTGGT CTTCTCCGAG 
AGCGTCCAGG TGGAGAAAGG CGACACCGAG CAGGAGATTG AGAGGCTCAG GGGCAGTCTG 
GAGGCTGAGA GCCGAGGCAA GAGGGAGCTG GACGCCGAGG TGAGCCGGCT GGAGGCCAAG 
CTGTCGGAGC TGGAGTTCTG CAACTCTAAG TCATCCAAGG AGCTGGACTT CCTGAGGGAG 
GAGAACCACA AGCTGCAGCT GGAGCGGCAG AGCCTGCAGC TCGAGGCCCG CAGGCTGCAG 
TCGGAGATCG AGATGGCCAA GGCCGAGGCG CAGAGCCTAA GAAGCCTGCC CACAACGACT 
GGAGCCGACC CCGGGGCGCA GCTCGACTCA CGCCTGCGGT CCCTGGAGCG GGAGCTGGAC 
GACCTCAGGA GGCTCTCCAG GGACAAAGAC CAGGAGATCG AAGAGCTGCA GCGTCGCCTG 
GGGTCCGTGG CCATCAAGCG GGAGCAGAGG GAGAACCACC TGCGGCGCTC CATTGTGGTC 
ATCGACCCTG ACTCGGGCCG TGAGCTGTCC CCCGAGGAGG CCCACCGCGC CGGCCTCATC 
GACTGGAACA TGTTTGTCAA GCTCAGGAGC CAGGAGTGCG ACTGGGAGGA GATCTCCGTC 
AAGGGCCCCG GAGGCGAGTC CTCCGTGATC CATGACAGGA AGTCCGGCAG GAAGTTCTCC 
ATTGAAGAGG CCCTGCAGAG CGGGCGGCTG ACCCCCGCGC AGTACGACCG CTACGTCAAC 
AAGAGCATGT CCATCCAGGA GCTGGCCGTC CTGGTGTCAG GGCAGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224719.1
CDS	207..5477
Translation	MNSLFRKRNKGKYSPTVRTRSVSNKELSELIEQLQKNADQVERNIVDTEAKMQRDLARLQEGQQPEHRDVALQKVSDSEKLLYMLEADAAIAKHMKHPQGDMIAEDIRQLKERVTNLRGKHKQIYNLVVKEVDPQVNWEALVEEKLDELSSQGFGTELPLVGQQVEQHNVFHNEVKAIGPHLAKEQNGELQAKYQKLLAASQARQQHLSSLQDYMQRCTNELYWLDQQAQGRIQYDWSDRNLNYPSRRRQYENFIHRNLEAKEERINKLHSEGDQLLAAEHPGRNSIEAHMEAVHADWKEYLNLLICEESHLKYMEDYHQFHKDMKDAQELLRKVDSDLNQKYSPDFKDRYQIELLLRELDDQEKALDKYQHVVRGLQKRGQQVVPLKYRRETPLKPIPVEALCDFEGDQGLISRGYSYTLQRNNGESWDLTDSAGNKLTAPAVCFMIPPTDPEALALAESLGSQYQSLRQKAAGSRKALQEQHEVLKAESSGADASDLQGRQLLAGLDKVASDLDRQEKAITAILRPPLEQGRAVQDSAERAKDLKNITNELLRIEPEKARSTAEGEAFVRALPDSGSTALLRTRVEDTQRRYERLVQLLEAAQEKLDIANRLEKSLQQGREMLATYETQLAQEDTVPESGQALDSRRQELAAMASELQARKALLGEVQRNLQAAKQCSGSLASRFQEHCPDLERQEAEVHKLCQRFDGLCQQAELRVQSLQSARAEHDAFCSGRDRLLQFLSHIPSYEPQETDGLGQVETKLNNQKNLLDEIARREEEVQKVHTHSQQYQQAVKDYELEAEKLRSLLDLENGRSSHVSKRARLQSPATKVREEEAALAAKFTEVNAINRQRLQNLEFALTLLRQQLEAGASQEALPGSKPGPGAEETWKIQEELDEEMERRQQLEHEVRSAQEEIRALRSQSPQEAVVTREVLRKVPDPALEESFQQAQQTLAEEQHKNRLLQGEVEALRLRLRALEEAARAGGQEYVVKEVLRIEPDRAQADEVLRLRGELEVLRRQKGAREAEALLLQQRVAALAEEKRRAREVVTEKEVVKVHNDPQLEAEFRQLQEDQQREQGLREKQEEELGFLQDKLKRLEKERAMAEGKITVKEVLKVEKDVAAEREVGALQRRYEDEAARARASQREKTELLRKIWALEEENAKVVVQEKVREIVRPDPKAESEVANLRLELVEQERKYRGAEEQLKSYQSELEALRRRGPQVEVKEVTKEVIKYTTDPETEKELQRLREEIVDKTRLIERCDLEIYQLKQEIQSLKEAKPQVQTKEVVQEILQFQEDPQTKEEVAALRARLAEEHKKQVDLERECVSQEEKIQEKEEELARGKEVVVQQEVVRYEEEPGLRAEVSAFTESIDAELRQIDGLRGELRRLQRRRAELEHQLEELERERQARREAELEVQHLKQQLAQLEAQEGEAHEKVTLTQKVVLQQDPQQAREHALLALQLEEERHRRQGLERELETLKQQLEHLEKMEVKEKVVFSESVQVEKGDTEQEIERLRGSLEAESRGKRELDAEVSRLEAKLSELEFCNSKSSKELDFLREENHKLQLERQSLQLEARRLQSEIEMAKAEAQSLRSLPTTTGADPGAQLDSRLRSLERELDDLRRLSRDKDQEIEELQRRLGSVAIKREQRENHLRRSIVVIDPDSGRELSPEEAHRAGLIDWNMFVKLRSQECDWEEISVKGPGGESSVIHDRKSGRKFSIEEALQSGRLTPAQYDRYVNKSMSIQELAVLVSGQK

Target ORF information:

RefSeq Version	XM_005224662.3
Organism	Bos taurus(cattle)
Definition	Bos taurus periplakin (PPL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224662.3

ORF Insert Sequence:

ATGAACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCCACGGT TCGGACCCGC 
AGCGTCTCTA ACAAGGAGCT GTCGGAGCTC ATCGAGCAGT TGCAGAAGAA TGCCGACCAG 
GTGGAGAGGA ACATCGTGGA CACGGAAGCC AAGATGCAGA GAGACCTGGC CCGGCTGCAG 
GAGGGCCAGC AGCCCGAGCA CCGGGATGTG GCCCTGCAGA AGGTGTCAGA CTCTGAGAAG 
CTGCTCTACA TGCTGGAGGC GGACGCGGCC ATCGCCAAGC ACATGAAGCA CCCCCAGGGG 
GACATGATCG CCGAGGACAT CCGTCAGCTG AAGGAGCGTG TGACCAACCT CCGCGGGAAA 
CACAAGCAGA TCTACAACCT GGTGGTGAAA GAGGTGGACC CACAGGTCAA CTGGGAGGCG 
CTGGTGGAGG AAAAGCTGGA CGAGCTGAGC AGCCAGGGCT TCGGGACGGA ACTCCCGCTG 
GTAGGCCAGC AGGTGGAGCA ACACAACGTC TTTCACAACG AGGTCAAGGC CATCGGGCCC 
CACCTGGCCA AGGAGCAGAA CGGTGAACTC CAGGCCAAGT ACCAGAAACT GCTGGCGGCG 
TCACAGGCGC GGCAGCAGCA CCTGAGCTCA CTGCAGGACT ACATGCAGCG GTGCACGAAC 
GAGCTGTACT GGCTGGACCA GCAGGCCCAG GGCCGCATAC AGTATGACTG GAGTGACCGC 
AACCTCAACT ACCCCAGCCG CCGGCGCCAG TACGAGAATT TCATCCACCG GAACCTGGAG 
GCCAAAGAGG AGAGAATTAA CAAGCTGCAC AGCGAGGGTG ACCAGCTGCT CGCCGCCGAG 
CACCCCGGGA GGAACTCCAT TGAAGCTCAC ATGGAGGCTG TCCACGCAGA TTGGAAGGAA 
TACCTGAACC TGCTCATCTG CGAGGAGAGC CACCTGAAGT ACATGGAAGA CTACCACCAG 
TTCCACAAAG ACATGAAGGA TGCCCAGGAG CTGCTTCGCA AGGTGGACTC AGACCTGAAC 
CAGAAATACA GCCCTGACTT CAAGGACCGA TACCAGATTG AGCTGCTGCT GCGGGAGCTG 
GATGACCAGG AGAAGGCTCT GGACAAGTAC CAGCATGTGG TGCGGGGGCT GCAGAAGCGA 
GGGCAGCAGG TGGTGCCCCT CAAGTACCGC CGGGAGACGC CCCTCAAGCC CATCCCCGTG 
GAGGCGCTCT GTGACTTCGA GGGTGACCAG GGCCTGATCT CTCGGGGCTA CAGCTACACC 
CTGCAGAGGA ACAATGGGGA GAGCTGGGAC CTCACCGACA GTGCGGGGAA CAAGCTGACC 
GCCCCGGCCG TCTGCTTCAT GATCCCGCCC ACTGACCCCG AGGCCCTGGC TCTGGCTGAA 
AGCCTGGGCA GCCAGTACCA GAGCCTGCGG CAGAAGGCAG CTGGGAGCAG AAAGGCGCTC 
CAGGAGCAGC ATGAGGTGCT GAAGGCAGAG AGCTCTGGAG CAGATGCCTC GGACCTGCAG 
GGCCGCCAGC TGCTGGCCGG CTTGGACAAG GTGGCCAGCG ACCTGGACCG GCAGGAGAAG 
GCCATCACGG CCATCCTGCG GCCGCCGCTG GAGCAGGGCC GGGCTGTGCA GGACAGCGCC 
GAGAGGGCCA AGGACCTCAA GAACATCACC AACGAGCTGC TGCGGATCGA GCCCGAGAAG 
GCGCGCAGCA CGGCCGAGGG CGAGGCGTTC GTGCGGGCCC TCCCGGACAG CGGCAGCACC 
GCCCTGCTGA GGACGCGGGT GGAGGACACC CAGCGCAGAT ACGAGCGCCT GGTGCAGCTG 
CTGGAAGCCG CCCAGGAGAA GCTCGACATC GCCAACCGCC TGGAGAAGAG CCTGCAGCAG 
GGCCGGGAGA TGCTGGCCAC GTATGAGACC CAGCTGGCCC AGGAGGACAC GGTGCCCGAG 
AGTGGCCAGG CCCTGGACAG CAGGAGGCAG GAGCTGGCGG CCATGGCCTC CGAGCTGCAG 
GCCCGGAAGG CCCTCCTCGG CGAGGTGCAG CGGAACCTGC AGGCCGCCAA GCAGTGCTCG 
GGCTCGCTGG CCAGCCGCTT CCAGGAGCAC TGCCCGGACC TGGAGCGCCA GGAGGCCGAG 
GTGCACAAGC TGTGCCAGCG CTTCGACGGC CTCTGCCAGC AGGCGGAGCT CAGGGTCCAG 
AGCCTGCAGA GCGCCCGGGC TGAGCACGAC GCCTTCTGCA GCGGCCGTGA CCGCCTGCTT 
CAGTTCCTGT CCCACATCCC CAGCTACGAG CCCCAGGAGA CGGACGGCCT CGGCCAAGTG 
GAGACCAAGC TGAACAACCA GAAGAACCTA CTAGATGAAA TAGCAAGAAG GGAAGAGGAA 
GTGCAGAAAG TCCACACGCA CTCCCAGCAG TACCAGCAGG CTGTCAAGGA CTACGAGTTG 
GAAGCAGAGA AACTCAGGTC CCTCCTTGAC TTGGAGAATG GAAGGAGCAG CCACGTGAGC 
AAGCGGGCCA GGCTCCAGTC CCCTGCCACC AAAGTGAGGG AAGAGGAAGC AGCTCTTGCT 
GCCAAGTTTA CGGAGGTTAA TGCCATCAAC AGACAGAGAC TGCAGAACCT GGAGTTTGCA 
CTGACCCTCC TGAGACAGCA GCTGGAGGCA GGTGCAAGCC AGGAGGCCCT GCCCGGGAGC 
AAGCCAGGCC CTGGAGCAGA GGAGACGTGG AAGATTCAGG AGGAGCTGGA TGAGGAGATG 
GAGCGGCGGC AGCAGCTGGA GCACGAGGTC CGGAGCGCCC AGGAGGAGAT CCGGGCCCTG 
CGGAGCCAGA GCCCGCAGGA GGCGGTGGTG ACCAGGGAGG TGCTCAGGAA GGTGCCGGAC 
CCAGCACTGG AGGAGAGCTT CCAGCAGGCG CAGCAGACCC TGGCCGAGGA GCAGCACAAG 
AACCGGCTGC TGCAGGGGGA GGTGGAGGCG CTGCGGCTGC GGCTGCGTGC CCTGGAGGAG 
GCGGCCCGGG CCGGGGGCCA GGAGTACGTG GTCAAGGAGG TACTGCGCAT TGAGCCGGAT 
CGGGCCCAGG CTGACGAGGT CCTGAGGCTG CGGGGGGAGC TGGAGGTGCT GCGGCGGCAG 
AAGGGCGCCC GCGAGGCGGA GGCGCTTCTC CTGCAGCAGC GCGTGGCCGC CCTGGCCGAG 
GAGAAGCGGC GGGCGCGGGA GGTGGTCACA GAGAAGGAGG TGGTCAAGGT GCACAATGAC 
CCCCAGCTGG AGGCCGAGTT CCGGCAGCTG CAGGAGGACC AGCAGCGGGA GCAGGGGCTC 
CGGGAGAAGC AGGAGGAGGA GCTGGGCTTC CTGCAGGACA AGCTCAAGAG GCTGGAGAAG 
GAACGGGCCA TGGCCGAGGG CAAGATCACA GTCAAGGAGG TGCTCAAGGT GGAGAAGGAC 
GTGGCGGCTG AGCGGGAGGT GGGCGCCCTC CAGCGCCGGT ATGAGGATGA GGCCGCCAGG 
GCCCGTGCCA GCCAGAGGGA GAAGACCGAG CTGCTCCGGA AGATCTGGGC CCTGGAGGAG 
GAGAACGCTA AGGTGGTTGT GCAAGAGAAG GTGCGCGAGA TTGTCCGGCC TGACCCCAAG 
GCTGAGAGTG AGGTGGCCAA CCTCCGCCTG GAGCTGGTAG AGCAGGAGCG CAAGTACCGG 
GGGGCTGAGG AGCAGCTCAA GAGCTACCAG AGCGAGCTGG AGGCGCTTCG GAGGCGAGGC 
CCCCAGGTAG AGGTCAAGGA GGTGACCAAG GAGGTCATCA AGTACACGAC GGACCCTGAG 
ACGGAGAAGG AGCTCCAGAG GCTCAGGGAG GAGATCGTGG ACAAGACCCG CCTCATCGAG 
AGGTGTGACC TGGAGATCTA CCAGCTGAAG CAGGAGATCC AGTCCCTCAA AGAGGCCAAG 
CCCCAGGTGC AGACCAAGGA GGTGGTCCAG GAGATCTTGC AGTTCCAGGA AGACCCCCAG 
ACCAAAGAGG AAGTGGCGGC TCTGAGGGCC CGGCTGGCTG AGGAGCACAA GAAGCAGGTA 
GACCTGGAAC GGGAATGCGT GTCACAGGAG GAGAAGATCC AGGAGAAGGA GGAGGAGCTG 
GCCCGCGGGA AGGAGGTGGT GGTGCAGCAG GAGGTGGTCA GGTACGAGGA GGAGCCGGGC 
CTCCGGGCGG AAGTGAGCGC CTTCACCGAG AGCATCGATG CAGAACTGCG GCAGATCGAC 
GGCCTCCGCG GCGAGCTGCG GCGGCTGCAG CGCCGGCGAG CCGAGCTGGA GCACCAGCTG 
GAGGAGCTGG AGCGCGAGCG GCAGGCGCGC CGCGAGGCTG AGCTGGAGGT GCAGCACCTC 
AAGCAACAGC TGGCCCAGCT GGAGGCGCAG GAGGGCGAGG CCCACGAGAA GGTGACCCTC 
ACACAGAAGG TGGTGCTGCA GCAGGACCCC CAGCAGGCCC GGGAGCATGC CCTCCTGGCC 
CTGCAGCTGG AGGAAGAGCG GCACCGCCGG CAGGGCCTGG AGCGCGAGCT CGAGACCCTA 
AAGCAGCAGC TGGAGCACCT GGAGAAGATG GAGGTCAAGG AGAAGGTGGT CTTCTCCGAG 
AGCGTCCAGG TGGAGAAAGG CGACACCGAG CAGGAGATTG AGAGGCTCAG GGGCAGTCTG 
GAGGCTGAGA GCCGAGGCAA GAGGGAGCTG GACGCCGAGG TGAGCCGGCT GGAGGCCAAG 
CTGTCGGAGC TGGAGTTCTG CAACTCTAAG TCATCCAAGG AGCTGGACTT CCTGAGGGAG 
GAGAACCACA AGCTGCAGCT GGAGCGGCAG AGCCTGCAGC TCGAGGCCCG CAGGCTGCAG 
TCGGAGATCG AGATGGCCAA GGCCGAGGCG CAGAGCCTAA GAAGCCTGCC CACAACGACT 
GGAGCCGACC CCGGGGCGCA GCTCGACTCA CGCCTGCGGT CCCTGGAGCG GGAGCTGGAC 
GACCTCAGGA GGCTCTCCAG GGACAAAGAC CAGGAGATCG AAGAGCTGCA GCGTCGCCTG 
GGGTCCGTGG CCATCAAGCG GGAGCAGAGG GAGAACCACC TGCGGCGCTC CATTGTGGTC 
ATCGACCCTG ACTCGGGCCG TGAGCTGTCC CCCGAGGAGG CCCACCGCGC CGGCCTCATC 
GACTGGAACA TGTTTGTCAA GCTCAGGAGC CAGGAGTGCG ACTGGGAGGA GATCTCCGTC 
AAGGGCCCCG GAGGCGAGTC CTCCGTGATC CATGACAGGA AGTCCGGCAG GAAGTTCTCC 
ATTGAAGAGG CCCTGCAGAG CGGGCGGCTG ACCCCCGCGC AGTACGACCG CTACGTCAAC 
AAGAGCATGT CCATCCAGGA GCTGGCCGTC CTGGTGTCAG GGCAGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPL ( XM_010799950.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010798252.2 Bos taurus periplakin (PPL), transcript variant X1, mRNA.

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CloneID	OBa32912
Clone ID Related Accession (Same CDS sequence)	XM_005224662.4 , XM_005224662.3 , XM_010799950.2
Accession Version	XM_010799950.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	periplakin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646645.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010799950.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCCACGGT TCGGACCCGC 
AGCGTCTCTA ACAAGGAGCT GTCGGAGCTC ATCGAGCAGT TGCAGAAGAA TGCCGACCAG 
GTGGAGAGGA ACATCGTGGA CACGGAAGCC AAGATGCAGA GAGACCTGGC CCGGCTGCAG 
GAGGGCCAGC AGCCCGAGCA CCGGGATGTG GCCCTGCAGA AGGTGTCAGA CTCTGAGAAG 
CTGCTCTACA TGCTGGAGGC GGACGCGGCC ATCGCCAAGC ACATGAAGCA CCCCCAGGGG 
GACATGATCG CCGAGGACAT CCGTCAGCTG AAGGAGCGTG TGACCAACCT CCGCGGGAAA 
CACAAGCAGA TCTACAACCT GGTGGTGAAA GAGGTGGACC CACAGGTCAA CTGGGAGGCG 
CTGGTGGAGG AAAAGCTGGA CGAGCTGAGC AGCCAGGGCT TCGGGACGGA ACTCCCGCTG 
GTAGGCCAGC AGGTGGAGCA ACACAACGTC TTTCACAACG AGGTCAAGGC CATCGGGCCC 
CACCTGGCCA AGGAGCAGAA CGGTGAACTC CAGGCCAAGT ACCAGAAACT GCTGGCGGCG 
TCACAGGCGC GGCAGCAGCA CCTGAGCTCA CTGCAGGACT ACATGCAGCG GTGCACGAAC 
GAGCTGTACT GGCTGGACCA GCAGGCCCAG GGCCGCATAC AGTATGACTG GAGTGACCGC 
AACCTCAACT ACCCCAGCCG CCGGCGCCAG TACGAGAATT TCATCCACCG GAACCTGGAG 
GCCAAAGAGG AGAGAATTAA CAAGCTGCAC AGCGAGGGTG ACCAGCTGCT CGCCGCCGAG 
CACCCCGGGA GGAACTCCAT TGAAGCTCAC ATGGAGGCTG TCCACGCAGA TTGGAAGGAA 
TACCTGAACC TGCTCATCTG CGAGGAGAGC CACCTGAAGT ACATGGAAGA CTACCACCAG 
TTCCACAAAG ACATGAAGGA TGCCCAGGAG CTGCTTCGCA AGGTGGACTC AGACCTGAAC 
CAGAAATACA GCCCTGACTT CAAGGACCGA TACCAGATTG AGCTGCTGCT GCGGGAGCTG 
GATGACCAGG AGAAGGCTCT GGACAAGTAC CAGCATGTGG TGCGGGGGCT GCAGAAGCGA 
GGGCAGCAGG TGGTGCCCCT CAAGTACCGC CGGGAGACGC CCCTCAAGCC CATCCCCGTG 
GAGGCGCTCT GTGACTTCGA GGGTGACCAG GGCCTGATCT CTCGGGGCTA CAGCTACACC 
CTGCAGAGGA ACAATGGGGA GAGCTGGGAC CTCACCGACA GTGCGGGGAA CAAGCTGACC 
GCCCCGGCCG TCTGCTTCAT GATCCCGCCC ACTGACCCCG AGGCCCTGGC TCTGGCTGAA 
AGCCTGGGCA GCCAGTACCA GAGCCTGCGG CAGAAGGCAG CTGGGAGCAG AAAGGCGCTC 
CAGGAGCAGC ATGAGGTGCT GAAGGCAGAG AGCTCTGGAG CAGATGCCTC GGACCTGCAG 
GGCCGCCAGC TGCTGGCCGG CTTGGACAAG GTGGCCAGCG ACCTGGACCG GCAGGAGAAG 
GCCATCACGG CCATCCTGCG GCCGCCGCTG GAGCAGGGCC GGGCTGTGCA GGACAGCGCC 
GAGAGGGCCA AGGACCTCAA GAACATCACC AACGAGCTGC TGCGGATCGA GCCCGAGAAG 
GCGCGCAGCA CGGCCGAGGG CGAGGCGTTC GTGCGGGCCC TCCCGGACAG CGGCAGCACC 
GCCCTGCTGA GGACGCGGGT GGAGGACACC CAGCGCAGAT ACGAGCGCCT GGTGCAGCTG 
CTGGAAGCCG CCCAGGAGAA GCTCGACATC GCCAACCGCC TGGAGAAGAG CCTGCAGCAG 
GGCCGGGAGA TGCTGGCCAC GTATGAGACC CAGCTGGCCC AGGAGGACAC GGTGCCCGAG 
AGTGGCCAGG CCCTGGACAG CAGGAGGCAG GAGCTGGCGG CCATGGCCTC CGAGCTGCAG 
GCCCGGAAGG CCCTCCTCGG CGAGGTGCAG CGGAACCTGC AGGCCGCCAA GCAGTGCTCG 
GGCTCGCTGG CCAGCCGCTT CCAGGAGCAC TGCCCGGACC TGGAGCGCCA GGAGGCCGAG 
GTGCACAAGC TGTGCCAGCG CTTCGACGGC CTCTGCCAGC AGGCGGAGCT CAGGGTCCAG 
AGCCTGCAGA GCGCCCGGGC TGAGCACGAC GCCTTCTGCA GCGGCCGTGA CCGCCTGCTT 
CAGTTCCTGT CCCACATCCC CAGCTACGAG CCCCAGGAGA CGGACGGCCT CGGCCAAGTG 
GAGACCAAGC TGAACAACCA GAAGAACCTA CTAGATGAAA TAGCAAGAAG GGAAGAGGAA 
GTGCAGAAAG TCCACACGCA CTCCCAGCAG TACCAGCAGG CTGTCAAGGA CTACGAGTTG 
GAAGCAGAGA AACTCAGGTC CCTCCTTGAC TTGGAGAATG GAAGGAGCAG CCACGTGAGC 
AAGCGGGCCA GGCTCCAGTC CCCTGCCACC AAAGTGAGGG AAGAGGAAGC AGCTCTTGCT 
GCCAAGTTTA CGGAGGTTAA TGCCATCAAC AGACAGAGAC TGCAGAACCT GGAGTTTGCA 
CTGACCCTCC TGAGACAGCA GCTGGAGGCA GGTGCAAGCC AGGAGGCCCT GCCCGGGAGC 
AAGCCAGGCC CTGGAGCAGA GGAGACGTGG AAGATTCAGG AGGAGCTGGA TGAGGAGATG 
GAGCGGCGGC AGCAGCTGGA GCACGAGGTC CGGAGCGCCC AGGAGGAGAT CCGGGCCCTG 
CGGAGCCAGA GCCCGCAGGA GGCGGTGGTG ACCAGGGAGG TGCTCAGGAA GGTGCCGGAC 
CCAGCACTGG AGGAGAGCTT CCAGCAGGCG CAGCAGACCC TGGCCGAGGA GCAGCACAAG 
AACCGGCTGC TGCAGGGGGA GGTGGAGGCG CTGCGGCTGC GGCTGCGTGC CCTGGAGGAG 
GCGGCCCGGG CCGGGGGCCA GGAGTACGTG GTCAAGGAGG TACTGCGCAT TGAGCCGGAT 
CGGGCCCAGG CTGACGAGGT CCTGAGGCTG CGGGGGGAGC TGGAGGTGCT GCGGCGGCAG 
AAGGGCGCCC GCGAGGCGGA GGCGCTTCTC CTGCAGCAGC GCGTGGCCGC CCTGGCCGAG 
GAGAAGCGGC GGGCGCGGGA GGTGGTCACA GAGAAGGAGG TGGTCAAGGT GCACAATGAC 
CCCCAGCTGG AGGCCGAGTT CCGGCAGCTG CAGGAGGACC AGCAGCGGGA GCAGGGGCTC 
CGGGAGAAGC AGGAGGAGGA GCTGGGCTTC CTGCAGGACA AGCTCAAGAG GCTGGAGAAG 
GAACGGGCCA TGGCCGAGGG CAAGATCACA GTCAAGGAGG TGCTCAAGGT GGAGAAGGAC 
GTGGCGGCTG AGCGGGAGGT GGGCGCCCTC CAGCGCCGGT ATGAGGATGA GGCCGCCAGG 
GCCCGTGCCA GCCAGAGGGA GAAGACCGAG CTGCTCCGGA AGATCTGGGC CCTGGAGGAG 
GAGAACGCTA AGGTGGTTGT GCAAGAGAAG GTGCGCGAGA TTGTCCGGCC TGACCCCAAG 
GCTGAGAGTG AGGTGGCCAA CCTCCGCCTG GAGCTGGTAG AGCAGGAGCG CAAGTACCGG 
GGGGCTGAGG AGCAGCTCAA GAGCTACCAG AGCGAGCTGG AGGCGCTTCG GAGGCGAGGC 
CCCCAGGTAG AGGTCAAGGA GGTGACCAAG GAGGTCATCA AGTACACGAC GGACCCTGAG 
ACGGAGAAGG AGCTCCAGAG GCTCAGGGAG GAGATCGTGG ACAAGACCCG CCTCATCGAG 
AGGTGTGACC TGGAGATCTA CCAGCTGAAG CAGGAGATCC AGTCCCTCAA AGAGGCCAAG 
CCCCAGGTGC AGACCAAGGA GGTGGTCCAG GAGATCTTGC AGTTCCAGGA AGACCCCCAG 
ACCAAAGAGG AAGTGGCGGC TCTGAGGGCC CGGCTGGCTG AGGAGCACAA GAAGCAGGTA 
GACCTGGAAC GGGAATGCGT GTCACAGGAG GAGAAGATCC AGGAGAAGGA GGAGGAGCTG 
GCCCGCGGGA AGGAGGTGGT GGTGCAGCAG GAGGTGGTCA GGTACGAGGA GGAGCCGGGC 
CTCCGGGCGG AAGTGAGCGC CTTCACCGAG AGCATCGATG CAGAACTGCG GCAGATCGAC 
GGCCTCCGCG GCGAGCTGCG GCGGCTGCAG CGCCGGCGAG CCGAGCTGGA GCACCAGCTG 
GAGGAGCTGG AGCGCGAGCG GCAGGCGCGC CGCGAGGCTG AGCTGGAGGT GCAGCACCTC 
AAGCAACAGC TGGCCCAGCT GGAGGCGCAG GAGGGCGAGG CCCACGAGAA GGTGACCCTC 
ACACAGAAGG TGGTGCTGCA GCAGGACCCC CAGCAGGCCC GGGAGCATGC CCTCCTGGCC 
CTGCAGCTGG AGGAAGAGCG GCACCGCCGG CAGGGCCTGG AGCGCGAGCT CGAGACCCTA 
AAGCAGCAGC TGGAGCACCT GGAGAAGATG GAGGTCAAGG AGAAGGTGGT CTTCTCCGAG 
AGCGTCCAGG TGGAGAAAGG CGACACCGAG CAGGAGATTG AGAGGCTCAG GGGCAGTCTG 
GAGGCTGAGA GCCGAGGCAA GAGGGAGCTG GACGCCGAGG TGAGCCGGCT GGAGGCCAAG 
CTGTCGGAGC TGGAGTTCTG CAACTCTAAG TCATCCAAGG AGCTGGACTT CCTGAGGGAG 
GAGAACCACA AGCTGCAGCT GGAGCGGCAG AGCCTGCAGC TCGAGGCCCG CAGGCTGCAG 
TCGGAGATCG AGATGGCCAA GGCCGAGGCG CAGAGCCTAA GAAGCCTGCC CACAACGACT 
GGAGCCGACC CCGGGGCGCA GCTCGACTCA CGCCTGCGGT CCCTGGAGCG GGAGCTGGAC 
GACCTCAGGA GGCTCTCCAG GGACAAAGAC CAGGAGATCG AAGAGCTGCA GCGTCGCCTG 
GGGTCCGTGG CCATCAAGCG GGAGCAGAGG GAGAACCACC TGCGGCGCTC CATTGTGGTC 
ATCGACCCTG ACTCGGGCCG TGAGCTGTCC CCCGAGGAGG CCCACCGCGC CGGCCTCATC 
GACTGGAACA TGTTTGTCAA GCTCAGGAGC CAGGAGTGCG ACTGGGAGGA GATCTCCGTC 
AAGGGCCCCG GAGGCGAGTC CTCCGTGATC CATGACAGGA AGTCCGGCAG GAAGTTCTCC 
ATTGAAGAGG CCCTGCAGAG CGGGCGGCTG ACCCCCGCGC AGTACGACCG CTACGTCAAC 
AAGAGCATGT CCATCCAGGA GCTGGCCGTC CTGGTGTCAG GGCAGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010798252.2
CDS	207..5477
Translation	MNSLFRKRNKGKYSPTVRTRSVSNKELSELIEQLQKNADQVERNIVDTEAKMQRDLARLQEGQQPEHRDVALQKVSDSEKLLYMLEADAAIAKHMKHPQGDMIAEDIRQLKERVTNLRGKHKQIYNLVVKEVDPQVNWEALVEEKLDELSSQGFGTELPLVGQQVEQHNVFHNEVKAIGPHLAKEQNGELQAKYQKLLAASQARQQHLSSLQDYMQRCTNELYWLDQQAQGRIQYDWSDRNLNYPSRRRQYENFIHRNLEAKEERINKLHSEGDQLLAAEHPGRNSIEAHMEAVHADWKEYLNLLICEESHLKYMEDYHQFHKDMKDAQELLRKVDSDLNQKYSPDFKDRYQIELLLRELDDQEKALDKYQHVVRGLQKRGQQVVPLKYRRETPLKPIPVEALCDFEGDQGLISRGYSYTLQRNNGESWDLTDSAGNKLTAPAVCFMIPPTDPEALALAESLGSQYQSLRQKAAGSRKALQEQHEVLKAESSGADASDLQGRQLLAGLDKVASDLDRQEKAITAILRPPLEQGRAVQDSAERAKDLKNITNELLRIEPEKARSTAEGEAFVRALPDSGSTALLRTRVEDTQRRYERLVQLLEAAQEKLDIANRLEKSLQQGREMLATYETQLAQEDTVPESGQALDSRRQELAAMASELQARKALLGEVQRNLQAAKQCSGSLASRFQEHCPDLERQEAEVHKLCQRFDGLCQQAELRVQSLQSARAEHDAFCSGRDRLLQFLSHIPSYEPQETDGLGQVETKLNNQKNLLDEIARREEEVQKVHTHSQQYQQAVKDYELEAEKLRSLLDLENGRSSHVSKRARLQSPATKVREEEAALAAKFTEVNAINRQRLQNLEFALTLLRQQLEAGASQEALPGSKPGPGAEETWKIQEELDEEMERRQQLEHEVRSAQEEIRALRSQSPQEAVVTREVLRKVPDPALEESFQQAQQTLAEEQHKNRLLQGEVEALRLRLRALEEAARAGGQEYVVKEVLRIEPDRAQADEVLRLRGELEVLRRQKGAREAEALLLQQRVAALAEEKRRAREVVTEKEVVKVHNDPQLEAEFRQLQEDQQREQGLREKQEEELGFLQDKLKRLEKERAMAEGKITVKEVLKVEKDVAAEREVGALQRRYEDEAARARASQREKTELLRKIWALEEENAKVVVQEKVREIVRPDPKAESEVANLRLELVEQERKYRGAEEQLKSYQSELEALRRRGPQVEVKEVTKEVIKYTTDPETEKELQRLREEIVDKTRLIERCDLEIYQLKQEIQSLKEAKPQVQTKEVVQEILQFQEDPQTKEEVAALRARLAEEHKKQVDLERECVSQEEKIQEKEEELARGKEVVVQQEVVRYEEEPGLRAEVSAFTESIDAELRQIDGLRGELRRLQRRRAELEHQLEELERERQARREAELEVQHLKQQLAQLEAQEGEAHEKVTLTQKVVLQQDPQQAREHALLALQLEEERHRRQGLERELETLKQQLEHLEKMEVKEKVVFSESVQVEKGDTEQEIERLRGSLEAESRGKRELDAEVSRLEAKLSELEFCNSKSSKELDFLREENHKLQLERQSLQLEARRLQSEIEMAKAEAQSLRSLPTTTGADPGAQLDSRLRSLERELDDLRRLSRDKDQEIEELQRRLGSVAIKREQRENHLRRSIVVIDPDSGRELSPEEAHRAGLIDWNMFVKLRSQECDWEEISVKGPGGESSVIHDRKSGRKFSIEEALQSGRLTPAQYDRYVNKSMSIQELAVLVSGQK

Target ORF information:

RefSeq Version	XM_010799950.2
Organism	Bos taurus(cattle)
Definition	Bos taurus periplakin (PPL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010799950.2

ORF Insert Sequence:

ATGAACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCCACGGT TCGGACCCGC 
AGCGTCTCTA ACAAGGAGCT GTCGGAGCTC ATCGAGCAGT TGCAGAAGAA TGCCGACCAG 
GTGGAGAGGA ACATCGTGGA CACGGAAGCC AAGATGCAGA GAGACCTGGC CCGGCTGCAG 
GAGGGCCAGC AGCCCGAGCA CCGGGATGTG GCCCTGCAGA AGGTGTCAGA CTCTGAGAAG 
CTGCTCTACA TGCTGGAGGC GGACGCGGCC ATCGCCAAGC ACATGAAGCA CCCCCAGGGG 
GACATGATCG CCGAGGACAT CCGTCAGCTG AAGGAGCGTG TGACCAACCT CCGCGGGAAA 
CACAAGCAGA TCTACAACCT GGTGGTGAAA GAGGTGGACC CACAGGTCAA CTGGGAGGCG 
CTGGTGGAGG AAAAGCTGGA CGAGCTGAGC AGCCAGGGCT TCGGGACGGA ACTCCCGCTG 
GTAGGCCAGC AGGTGGAGCA ACACAACGTC TTTCACAACG AGGTCAAGGC CATCGGGCCC 
CACCTGGCCA AGGAGCAGAA CGGTGAACTC CAGGCCAAGT ACCAGAAACT GCTGGCGGCG 
TCACAGGCGC GGCAGCAGCA CCTGAGCTCA CTGCAGGACT ACATGCAGCG GTGCACGAAC 
GAGCTGTACT GGCTGGACCA GCAGGCCCAG GGCCGCATAC AGTATGACTG GAGTGACCGC 
AACCTCAACT ACCCCAGCCG CCGGCGCCAG TACGAGAATT TCATCCACCG GAACCTGGAG 
GCCAAAGAGG AGAGAATTAA CAAGCTGCAC AGCGAGGGTG ACCAGCTGCT CGCCGCCGAG 
CACCCCGGGA GGAACTCCAT TGAAGCTCAC ATGGAGGCTG TCCACGCAGA TTGGAAGGAA 
TACCTGAACC TGCTCATCTG CGAGGAGAGC CACCTGAAGT ACATGGAAGA CTACCACCAG 
TTCCACAAAG ACATGAAGGA TGCCCAGGAG CTGCTTCGCA AGGTGGACTC AGACCTGAAC 
CAGAAATACA GCCCTGACTT CAAGGACCGA TACCAGATTG AGCTGCTGCT GCGGGAGCTG 
GATGACCAGG AGAAGGCTCT GGACAAGTAC CAGCATGTGG TGCGGGGGCT GCAGAAGCGA 
GGGCAGCAGG TGGTGCCCCT CAAGTACCGC CGGGAGACGC CCCTCAAGCC CATCCCCGTG 
GAGGCGCTCT GTGACTTCGA GGGTGACCAG GGCCTGATCT CTCGGGGCTA CAGCTACACC 
CTGCAGAGGA ACAATGGGGA GAGCTGGGAC CTCACCGACA GTGCGGGGAA CAAGCTGACC 
GCCCCGGCCG TCTGCTTCAT GATCCCGCCC ACTGACCCCG AGGCCCTGGC TCTGGCTGAA 
AGCCTGGGCA GCCAGTACCA GAGCCTGCGG CAGAAGGCAG CTGGGAGCAG AAAGGCGCTC 
CAGGAGCAGC ATGAGGTGCT GAAGGCAGAG AGCTCTGGAG CAGATGCCTC GGACCTGCAG 
GGCCGCCAGC TGCTGGCCGG CTTGGACAAG GTGGCCAGCG ACCTGGACCG GCAGGAGAAG 
GCCATCACGG CCATCCTGCG GCCGCCGCTG GAGCAGGGCC GGGCTGTGCA GGACAGCGCC 
GAGAGGGCCA AGGACCTCAA GAACATCACC AACGAGCTGC TGCGGATCGA GCCCGAGAAG 
GCGCGCAGCA CGGCCGAGGG CGAGGCGTTC GTGCGGGCCC TCCCGGACAG CGGCAGCACC 
GCCCTGCTGA GGACGCGGGT GGAGGACACC CAGCGCAGAT ACGAGCGCCT GGTGCAGCTG 
CTGGAAGCCG CCCAGGAGAA GCTCGACATC GCCAACCGCC TGGAGAAGAG CCTGCAGCAG 
GGCCGGGAGA TGCTGGCCAC GTATGAGACC CAGCTGGCCC AGGAGGACAC GGTGCCCGAG 
AGTGGCCAGG CCCTGGACAG CAGGAGGCAG GAGCTGGCGG CCATGGCCTC CGAGCTGCAG 
GCCCGGAAGG CCCTCCTCGG CGAGGTGCAG CGGAACCTGC AGGCCGCCAA GCAGTGCTCG 
GGCTCGCTGG CCAGCCGCTT CCAGGAGCAC TGCCCGGACC TGGAGCGCCA GGAGGCCGAG 
GTGCACAAGC TGTGCCAGCG CTTCGACGGC CTCTGCCAGC AGGCGGAGCT CAGGGTCCAG 
AGCCTGCAGA GCGCCCGGGC TGAGCACGAC GCCTTCTGCA GCGGCCGTGA CCGCCTGCTT 
CAGTTCCTGT CCCACATCCC CAGCTACGAG CCCCAGGAGA CGGACGGCCT CGGCCAAGTG 
GAGACCAAGC TGAACAACCA GAAGAACCTA CTAGATGAAA TAGCAAGAAG GGAAGAGGAA 
GTGCAGAAAG TCCACACGCA CTCCCAGCAG TACCAGCAGG CTGTCAAGGA CTACGAGTTG 
GAAGCAGAGA AACTCAGGTC CCTCCTTGAC TTGGAGAATG GAAGGAGCAG CCACGTGAGC 
AAGCGGGCCA GGCTCCAGTC CCCTGCCACC AAAGTGAGGG AAGAGGAAGC AGCTCTTGCT 
GCCAAGTTTA CGGAGGTTAA TGCCATCAAC AGACAGAGAC TGCAGAACCT GGAGTTTGCA 
CTGACCCTCC TGAGACAGCA GCTGGAGGCA GGTGCAAGCC AGGAGGCCCT GCCCGGGAGC 
AAGCCAGGCC CTGGAGCAGA GGAGACGTGG AAGATTCAGG AGGAGCTGGA TGAGGAGATG 
GAGCGGCGGC AGCAGCTGGA GCACGAGGTC CGGAGCGCCC AGGAGGAGAT CCGGGCCCTG 
CGGAGCCAGA GCCCGCAGGA GGCGGTGGTG ACCAGGGAGG TGCTCAGGAA GGTGCCGGAC 
CCAGCACTGG AGGAGAGCTT CCAGCAGGCG CAGCAGACCC TGGCCGAGGA GCAGCACAAG 
AACCGGCTGC TGCAGGGGGA GGTGGAGGCG CTGCGGCTGC GGCTGCGTGC CCTGGAGGAG 
GCGGCCCGGG CCGGGGGCCA GGAGTACGTG GTCAAGGAGG TACTGCGCAT TGAGCCGGAT 
CGGGCCCAGG CTGACGAGGT CCTGAGGCTG CGGGGGGAGC TGGAGGTGCT GCGGCGGCAG 
AAGGGCGCCC GCGAGGCGGA GGCGCTTCTC CTGCAGCAGC GCGTGGCCGC CCTGGCCGAG 
GAGAAGCGGC GGGCGCGGGA GGTGGTCACA GAGAAGGAGG TGGTCAAGGT GCACAATGAC 
CCCCAGCTGG AGGCCGAGTT CCGGCAGCTG CAGGAGGACC AGCAGCGGGA GCAGGGGCTC 
CGGGAGAAGC AGGAGGAGGA GCTGGGCTTC CTGCAGGACA AGCTCAAGAG GCTGGAGAAG 
GAACGGGCCA TGGCCGAGGG CAAGATCACA GTCAAGGAGG TGCTCAAGGT GGAGAAGGAC 
GTGGCGGCTG AGCGGGAGGT GGGCGCCCTC CAGCGCCGGT ATGAGGATGA GGCCGCCAGG 
GCCCGTGCCA GCCAGAGGGA GAAGACCGAG CTGCTCCGGA AGATCTGGGC CCTGGAGGAG 
GAGAACGCTA AGGTGGTTGT GCAAGAGAAG GTGCGCGAGA TTGTCCGGCC TGACCCCAAG 
GCTGAGAGTG AGGTGGCCAA CCTCCGCCTG GAGCTGGTAG AGCAGGAGCG CAAGTACCGG 
GGGGCTGAGG AGCAGCTCAA GAGCTACCAG AGCGAGCTGG AGGCGCTTCG GAGGCGAGGC 
CCCCAGGTAG AGGTCAAGGA GGTGACCAAG GAGGTCATCA AGTACACGAC GGACCCTGAG 
ACGGAGAAGG AGCTCCAGAG GCTCAGGGAG GAGATCGTGG ACAAGACCCG CCTCATCGAG 
AGGTGTGACC TGGAGATCTA CCAGCTGAAG CAGGAGATCC AGTCCCTCAA AGAGGCCAAG 
CCCCAGGTGC AGACCAAGGA GGTGGTCCAG GAGATCTTGC AGTTCCAGGA AGACCCCCAG 
ACCAAAGAGG AAGTGGCGGC TCTGAGGGCC CGGCTGGCTG AGGAGCACAA GAAGCAGGTA 
GACCTGGAAC GGGAATGCGT GTCACAGGAG GAGAAGATCC AGGAGAAGGA GGAGGAGCTG 
GCCCGCGGGA AGGAGGTGGT GGTGCAGCAG GAGGTGGTCA GGTACGAGGA GGAGCCGGGC 
CTCCGGGCGG AAGTGAGCGC CTTCACCGAG AGCATCGATG CAGAACTGCG GCAGATCGAC 
GGCCTCCGCG GCGAGCTGCG GCGGCTGCAG CGCCGGCGAG CCGAGCTGGA GCACCAGCTG 
GAGGAGCTGG AGCGCGAGCG GCAGGCGCGC CGCGAGGCTG AGCTGGAGGT GCAGCACCTC 
AAGCAACAGC TGGCCCAGCT GGAGGCGCAG GAGGGCGAGG CCCACGAGAA GGTGACCCTC 
ACACAGAAGG TGGTGCTGCA GCAGGACCCC CAGCAGGCCC GGGAGCATGC CCTCCTGGCC 
CTGCAGCTGG AGGAAGAGCG GCACCGCCGG CAGGGCCTGG AGCGCGAGCT CGAGACCCTA 
AAGCAGCAGC TGGAGCACCT GGAGAAGATG GAGGTCAAGG AGAAGGTGGT CTTCTCCGAG 
AGCGTCCAGG TGGAGAAAGG CGACACCGAG CAGGAGATTG AGAGGCTCAG GGGCAGTCTG 
GAGGCTGAGA GCCGAGGCAA GAGGGAGCTG GACGCCGAGG TGAGCCGGCT GGAGGCCAAG 
CTGTCGGAGC TGGAGTTCTG CAACTCTAAG TCATCCAAGG AGCTGGACTT CCTGAGGGAG 
GAGAACCACA AGCTGCAGCT GGAGCGGCAG AGCCTGCAGC TCGAGGCCCG CAGGCTGCAG 
TCGGAGATCG AGATGGCCAA GGCCGAGGCG CAGAGCCTAA GAAGCCTGCC CACAACGACT 
GGAGCCGACC CCGGGGCGCA GCTCGACTCA CGCCTGCGGT CCCTGGAGCG GGAGCTGGAC 
GACCTCAGGA GGCTCTCCAG GGACAAAGAC CAGGAGATCG AAGAGCTGCA GCGTCGCCTG 
GGGTCCGTGG CCATCAAGCG GGAGCAGAGG GAGAACCACC TGCGGCGCTC CATTGTGGTC 
ATCGACCCTG ACTCGGGCCG TGAGCTGTCC CCCGAGGAGG CCCACCGCGC CGGCCTCATC 
GACTGGAACA TGTTTGTCAA GCTCAGGAGC CAGGAGTGCG ACTGGGAGGA GATCTCCGTC 
AAGGGCCCCG GAGGCGAGTC CTCCGTGATC CATGACAGGA AGTCCGGCAG GAAGTTCTCC 
ATTGAAGAGG CCCTGCAGAG CGGGCGGCTG ACCCCCGCGC AGTACGACCG CTACGTCAAC 
AAGAGCATGT CCATCCAGGA GCTGGCCGTC CTGGTGTCAG GGCAGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPL ( XM_015469294.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324780.1 Bos taurus periplakin (PPL), transcript variant X2, mRNA.

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CloneID	OBa32913
Clone ID Related Accession (Same CDS sequence)	XM_015469294.1 , XM_002697888.5 , XM_002697888.6
Accession Version	XM_015469294.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	periplakin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646645.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCCACGGT TCGGACCCGC 
AGCGTCTCTA ACAAGGAGCT GTCGGAGCTC ATCGAGCAGT TGCAGAAGAA TGCCGACCAG 
GTGGAGAGGA ACATCGTGGA CACGGAAGCC AAGATGCAGA GAGACCTGGC CCGGCTGCAG 
GAGGGCCAGC AGCCCGAGCA CCGGGATGTG GCCCTGCAGA AGGTGTCAGA CTCTGAGAAG 
CTGCTCTACA TGCTGGAGGC GGACGCGGCC ATCGCCAAGC ACATGAAGCA CCCCCAGGGG 
GACATGATCG CCGAGGACAT CCGTCAGCTG AAGGAGCGTG TGACCAACCT CCGCGGGAAA 
CACAAGCAGA TCTACAACCT GGTGGTGAAA GAGGTGGACC CACAGGTCAA CTGGGAGGCG 
CTGGTGGAGG AAAAGCTGGA CGAGCTGAGC AGCCAGGGCT TCGGGACGGA ACTCCCGCTG 
GTAGGCCAGC AGGTGGAGCA ACACAACGTC TTTCACAACG AGGTCAAGGC CATCGGGCCC 
CACCTGGCCA AGGAGCAGAA CGGTGAACTC CAGGCCAAGT ACCAGAAACT GCTGGCGGCG 
TCACAGGCGC GGCAGCAGCA CCTGAGCTCA CTGCAGGACT ACATGCAGCG GTGCACGAAC 
GAGCTGTACT GGCTGGACCA GCAGGCCCAG GGCCGCATAC AGTATGACTG GAGTGACCGC 
AACCTCAACT ACCCCAGCCG CCGGCGCCAG TACGAGAATT TCATCCACCG GAACCTGGAG 
GCCAAAGAGG AGAGAATTAA CAAGCTGCAC AGCGAGGGTG ACCAGCTGCT CGCCGCCGAG 
CACCCCGGGA GGAACTCCAT TGAAGCTCAC ATGGAGGCTG TCCACGCAGA TTGGAAGGAA 
TACCTGAACC TGCTCATCTG CGAGGAGAGC CACCTGAAGT ACATGGAAGA CTACCACCAG 
TTCCACAAAG ACATGAAGGA TGCCCAGGAG CTGCTTCGCA AGGTGGACTC AGACCTGAAC 
CAGAAATACA GCCCTGACTT CAAGGACCGA TACCAGATTG AGCTGCTGCT GCGGGAGCTG 
GATGACCAGG AGAAGGCTCT GGACAAGTAC CAGCATGTGG TGCGGGGGCT GCAGAAGCGA 
GGGCAGCAGG TGGTGCCCCT CAAGTACCGC CGGGAGACGC CCCTCAAGCC CATCCCCGTG 
GAGGCGCTCT GTGACTTCGA GGGTGACCAG GGCCTGATCT CTCGGGGCTA CAGCTACACC 
CTGCAGAGGA ACAATGGGGA GAGCTGGGAC CTCACCGACA GTGCGGGGAA CAAGCTGACC 
GCCCCGGCCG TCTGCTTCAT GATCCCGCCC ACTGACCCCG AGGCCCTGGC TCTGGCTGAA 
AGCCTGGGCA GCCAGTACCA GAGCCTGCGG CAGAAGGCAG CTGGGAGCAG AAAGGCGCTC 
CAGGAGCAGC ATGAGGTGCT GAAGGCAGAG AGCTCTGGAG ATGCCTCGGA CCTGCAGGGC 
CGCCAGCTGC TGGCCGGCTT GGACAAGGTG GCCAGCGACC TGGACCGGCA GGAGAAGGCC 
ATCACGGCCA TCCTGCGGCC GCCGCTGGAG CAGGGCCGGG CTGTGCAGGA CAGCGCCGAG 
AGGGCCAAGG ACCTCAAGAA CATCACCAAC GAGCTGCTGC GGATCGAGCC CGAGAAGGCG 
CGCAGCACGG CCGAGGGCGA GGCGTTCGTG CGGGCCCTCC CGGACAGCGG CAGCACCGCC 
CTGCTGAGGA CGCGGGTGGA GGACACCCAG CGCAGATACG AGCGCCTGGT GCAGCTGCTG 
GAAGCCGCCC AGGAGAAGCT CGACATCGCC AACCGCCTGG AGAAGAGCCT GCAGCAGGGC 
CGGGAGATGC TGGCCACGTA TGAGACCCAG CTGGCCCAGG AGGACACGGT GCCCGAGAGT 
GGCCAGGCCC TGGACAGCAG GAGGCAGGAG CTGGCGGCCA TGGCCTCCGA GCTGCAGGCC 
CGGAAGGCCC TCCTCGGCGA GGTGCAGCGG AACCTGCAGG CCGCCAAGCA GTGCTCGGGC 
TCGCTGGCCA GCCGCTTCCA GGAGCACTGC CCGGACCTGG AGCGCCAGGA GGCCGAGGTG 
CACAAGCTGT GCCAGCGCTT CGACGGCCTC TGCCAGCAGG CGGAGCTCAG GGTCCAGAGC 
CTGCAGAGCG CCCGGGCTGA GCACGACGCC TTCTGCAGCG GCCGTGACCG CCTGCTTCAG 
TTCCTGTCCC ACATCCCCAG CTACGAGCCC CAGGAGACGG ACGGCCTCGG CCAAGTGGAG 
ACCAAGCTGA ACAACCAGAA GAACCTACTA GATGAAATAG CAAGAAGGGA AGAGGAAGTG 
CAGAAAGTCC ACACGCACTC CCAGCAGTAC CAGCAGGCTG TCAAGGACTA CGAGTTGGAA 
GCAGAGAAAC TCAGGTCCCT CCTTGACTTG GAGAATGGAA GGAGCAGCCA CGTGAGCAAG 
CGGGCCAGGC TCCAGTCCCC TGCCACCAAA GTGAGGGAAG AGGAAGCAGC TCTTGCTGCC 
AAGTTTACGG AGGTTAATGC CATCAACAGA CAGAGACTGC AGAACCTGGA GTTTGCACTG 
ACCCTCCTGA GACAGCAGCT GGAGGCAGGT GCAAGCCAGG AGGCCCTGCC CGGGAGCAAG 
CCAGGCCCTG GAGCAGAGGA GACGTGGAAG ATTCAGGAGG AGCTGGATGA GGAGATGGAG 
CGGCGGCAGC AGCTGGAGCA CGAGGTCCGG AGCGCCCAGG AGGAGATCCG GGCCCTGCGG 
AGCCAGAGCC CGCAGGAGGC GGTGGTGACC AGGGAGGTGC TCAGGAAGGT GCCGGACCCA 
GCACTGGAGG AGAGCTTCCA GCAGGCGCAG CAGACCCTGG CCGAGGAGCA GCACAAGAAC 
CGGCTGCTGC AGGGGGAGGT GGAGGCGCTG CGGCTGCGGC TGCGTGCCCT GGAGGAGGCG 
GCCCGGGCCG GGGGCCAGGA GTACGTGGTC AAGGAGGTAC TGCGCATTGA GCCGGATCGG 
GCCCAGGCTG ACGAGGTCCT GAGGCTGCGG GGGGAGCTGG AGGTGCTGCG GCGGCAGAAG 
GGCGCCCGCG AGGCGGAGGC GCTTCTCCTG CAGCAGCGCG TGGCCGCCCT GGCCGAGGAG 
AAGCGGCGGG CGCGGGAGGT GGTCACAGAG AAGGAGGTGG TCAAGGTGCA CAATGACCCC 
CAGCTGGAGG CCGAGTTCCG GCAGCTGCAG GAGGACCAGC AGCGGGAGCA GGGGCTCCGG 
GAGAAGCAGG AGGAGGAGCT GGGCTTCCTG CAGGACAAGC TCAAGAGGCT GGAGAAGGAA 
CGGGCCATGG CCGAGGGCAA GATCACAGTC AAGGAGGTGC TCAAGGTGGA GAAGGACGTG 
GCGGCTGAGC GGGAGGTGGG CGCCCTCCAG CGCCGGTATG AGGATGAGGC CGCCAGGGCC 
CGTGCCAGCC AGAGGGAGAA GACCGAGCTG CTCCGGAAGA TCTGGGCCCT GGAGGAGGAG 
AACGCTAAGG TGGTTGTGCA AGAGAAGGTG CGCGAGATTG TCCGGCCTGA CCCCAAGGCT 
GAGAGTGAGG TGGCCAACCT CCGCCTGGAG CTGGTAGAGC AGGAGCGCAA GTACCGGGGG 
GCTGAGGAGC AGCTCAAGAG CTACCAGAGC GAGCTGGAGG CGCTTCGGAG GCGAGGCCCC 
CAGGTAGAGG TCAAGGAGGT GACCAAGGAG GTCATCAAGT ACACGACGGA CCCTGAGACG 
GAGAAGGAGC TCCAGAGGCT CAGGGAGGAG ATCGTGGACA AGACCCGCCT CATCGAGAGG 
TGTGACCTGG AGATCTACCA GCTGAAGCAG GAGATCCAGT CCCTCAAAGA GGCCAAGCCC 
CAGGTGCAGA CCAAGGAGGT GGTCCAGGAG ATCTTGCAGT TCCAGGAAGA CCCCCAGACC 
AAAGAGGAAG TGGCGGCTCT GAGGGCCCGG CTGGCTGAGG AGCACAAGAA GCAGGTAGAC 
CTGGAACGGG AATGCGTGTC ACAGGAGGAG AAGATCCAGG AGAAGGAGGA GGAGCTGGCC 
CGCGGGAAGG AGGTGGTGGT GCAGCAGGAG GTGGTCAGGT ACGAGGAGGA GCCGGGCCTC 
CGGGCGGAAG TGAGCGCCTT CACCGAGAGC ATCGATGCAG AACTGCGGCA GATCGACGGC 
CTCCGCGGCG AGCTGCGGCG GCTGCAGCGC CGGCGAGCCG AGCTGGAGCA CCAGCTGGAG 
GAGCTGGAGC GCGAGCGGCA GGCGCGCCGC GAGGCTGAGC TGGAGGTGCA GCACCTCAAG 
CAACAGCTGG CCCAGCTGGA GGCGCAGGAG GGCGAGGCCC ACGAGAAGGT GACCCTCACA 
CAGAAGGTGG TGCTGCAGCA GGACCCCCAG CAGGCCCGGG AGCATGCCCT CCTGGCCCTG 
CAGCTGGAGG AAGAGCGGCA CCGCCGGCAG GGCCTGGAGC GCGAGCTCGA GACCCTAAAG 
CAGCAGCTGG AGCACCTGGA GAAGATGGAG GTCAAGGAGA AGGTGGTCTT CTCCGAGAGC 
GTCCAGGTGG AGAAAGGCGA CACCGAGCAG GAGATTGAGA GGCTCAGGGG CAGTCTGGAG 
GCTGAGAGCC GAGGCAAGAG GGAGCTGGAC GCCGAGGTGA GCCGGCTGGA GGCCAAGCTG 
TCGGAGCTGG AGTTCTGCAA CTCTAAGTCA TCCAAGGAGC TGGACTTCCT GAGGGAGGAG 
AACCACAAGC TGCAGCTGGA GCGGCAGAGC CTGCAGCTCG AGGCCCGCAG GCTGCAGTCG 
GAGATCGAGA TGGCCAAGGC CGAGGCGCAG AGCCTAAGAA GCCTGCCCAC AACGACTGGA 
GCCGACCCCG GGGCGCAGCT CGACTCACGC CTGCGGTCCC TGGAGCGGGA GCTGGACGAC 
CTCAGGAGGC TCTCCAGGGA CAAAGACCAG GAGATCGAAG AGCTGCAGCG TCGCCTGGGG 
TCCGTGGCCA TCAAGCGGGA GCAGAGGGAG AACCACCTGC GGCGCTCCAT TGTGGTCATC 
GACCCTGACT CGGGCCGTGA GCTGTCCCCC GAGGAGGCCC ACCGCGCCGG CCTCATCGAC 
TGGAACATGT TTGTCAAGCT CAGGAGCCAG GAGTGCGACT GGGAGGAGAT CTCCGTCAAG 
GGCCCCGGAG GCGAGTCCTC CGTGATCCAT GACAGGAAGT CCGGCAGGAA GTTCTCCATT 
GAAGAGGCCC TGCAGAGCGG GCGGCTGACC CCCGCGCAGT ACGACCGCTA CGTCAACAAG 
AGCATGTCCA TCCAGGAGCT GGCCGTCCTG GTGTCAGGGC AGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324780.1
CDS	207..5474
Translation	MNSLFRKRNKGKYSPTVRTRSVSNKELSELIEQLQKNADQVERNIVDTEAKMQRDLARLQEGQQPEHRDVALQKVSDSEKLLYMLEADAAIAKHMKHPQGDMIAEDIRQLKERVTNLRGKHKQIYNLVVKEVDPQVNWEALVEEKLDELSSQGFGTELPLVGQQVEQHNVFHNEVKAIGPHLAKEQNGELQAKYQKLLAASQARQQHLSSLQDYMQRCTNELYWLDQQAQGRIQYDWSDRNLNYPSRRRQYENFIHRNLEAKEERINKLHSEGDQLLAAEHPGRNSIEAHMEAVHADWKEYLNLLICEESHLKYMEDYHQFHKDMKDAQELLRKVDSDLNQKYSPDFKDRYQIELLLRELDDQEKALDKYQHVVRGLQKRGQQVVPLKYRRETPLKPIPVEALCDFEGDQGLISRGYSYTLQRNNGESWDLTDSAGNKLTAPAVCFMIPPTDPEALALAESLGSQYQSLRQKAAGSRKALQEQHEVLKAESSGDASDLQGRQLLAGLDKVASDLDRQEKAITAILRPPLEQGRAVQDSAERAKDLKNITNELLRIEPEKARSTAEGEAFVRALPDSGSTALLRTRVEDTQRRYERLVQLLEAAQEKLDIANRLEKSLQQGREMLATYETQLAQEDTVPESGQALDSRRQELAAMASELQARKALLGEVQRNLQAAKQCSGSLASRFQEHCPDLERQEAEVHKLCQRFDGLCQQAELRVQSLQSARAEHDAFCSGRDRLLQFLSHIPSYEPQETDGLGQVETKLNNQKNLLDEIARREEEVQKVHTHSQQYQQAVKDYELEAEKLRSLLDLENGRSSHVSKRARLQSPATKVREEEAALAAKFTEVNAINRQRLQNLEFALTLLRQQLEAGASQEALPGSKPGPGAEETWKIQEELDEEMERRQQLEHEVRSAQEEIRALRSQSPQEAVVTREVLRKVPDPALEESFQQAQQTLAEEQHKNRLLQGEVEALRLRLRALEEAARAGGQEYVVKEVLRIEPDRAQADEVLRLRGELEVLRRQKGAREAEALLLQQRVAALAEEKRRAREVVTEKEVVKVHNDPQLEAEFRQLQEDQQREQGLREKQEEELGFLQDKLKRLEKERAMAEGKITVKEVLKVEKDVAAEREVGALQRRYEDEAARARASQREKTELLRKIWALEEENAKVVVQEKVREIVRPDPKAESEVANLRLELVEQERKYRGAEEQLKSYQSELEALRRRGPQVEVKEVTKEVIKYTTDPETEKELQRLREEIVDKTRLIERCDLEIYQLKQEIQSLKEAKPQVQTKEVVQEILQFQEDPQTKEEVAALRARLAEEHKKQVDLERECVSQEEKIQEKEEELARGKEVVVQQEVVRYEEEPGLRAEVSAFTESIDAELRQIDGLRGELRRLQRRRAELEHQLEELERERQARREAELEVQHLKQQLAQLEAQEGEAHEKVTLTQKVVLQQDPQQAREHALLALQLEEERHRRQGLERELETLKQQLEHLEKMEVKEKVVFSESVQVEKGDTEQEIERLRGSLEAESRGKRELDAEVSRLEAKLSELEFCNSKSSKELDFLREENHKLQLERQSLQLEARRLQSEIEMAKAEAQSLRSLPTTTGADPGAQLDSRLRSLERELDDLRRLSRDKDQEIEELQRRLGSVAIKREQRENHLRRSIVVIDPDSGRELSPEEAHRAGLIDWNMFVKLRSQECDWEEISVKGPGGESSVIHDRKSGRKFSIEEALQSGRLTPAQYDRYVNKSMSIQELAVLVSGQK

Target ORF information:

RefSeq Version	XM_015469294.1
Organism	Bos taurus(cattle)
Definition	Bos taurus periplakin (PPL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469294.1

ORF Insert Sequence:

ATGAACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCCACGGT TCGGACCCGC 
AGCGTCTCTA ACAAGGAGCT GTCGGAGCTC ATCGAGCAGT TGCAGAAGAA TGCCGACCAG 
GTGGAGAGGA ACATCGTGGA CACGGAAGCC AAGATGCAGA GAGACCTGGC CCGGCTGCAG 
GAGGGCCAGC AGCCCGAGCA CCGGGATGTG GCCCTGCAGA AGGTGTCAGA CTCTGAGAAG 
CTGCTCTACA TGCTGGAGGC GGACGCGGCC ATCGCCAAGC ACATGAAGCA CCCCCAGGGG 
GACATGATCG CCGAGGACAT CCGTCAGCTG AAGGAGCGTG TGACCAACCT CCGCGGGAAA 
CACAAGCAGA TCTACAACCT GGTGGTGAAA GAGGTGGACC CACAGGTCAA CTGGGAGGCG 
CTGGTGGAGG AAAAGCTGGA CGAGCTGAGC AGCCAGGGCT TCGGGACGGA ACTCCCGCTG 
GTAGGCCAGC AGGTGGAGCA ACACAACGTC TTTCACAACG AGGTCAAGGC CATCGGGCCC 
CACCTGGCCA AGGAGCAGAA CGGTGAACTC CAGGCCAAGT ACCAGAAACT GCTGGCGGCG 
TCACAGGCGC GGCAGCAGCA CCTGAGCTCA CTGCAGGACT ACATGCAGCG GTGCACGAAC 
GAGCTGTACT GGCTGGACCA GCAGGCCCAG GGCCGCATAC AGTATGACTG GAGTGACCGC 
AACCTCAACT ACCCCAGCCG CCGGCGCCAG TACGAGAATT TCATCCACCG GAACCTGGAG 
GCCAAAGAGG AGAGAATTAA CAAGCTGCAC AGCGAGGGTG ACCAGCTGCT CGCCGCCGAG 
CACCCCGGGA GGAACTCCAT TGAAGCTCAC ATGGAGGCTG TCCACGCAGA TTGGAAGGAA 
TACCTGAACC TGCTCATCTG CGAGGAGAGC CACCTGAAGT ACATGGAAGA CTACCACCAG 
TTCCACAAAG ACATGAAGGA TGCCCAGGAG CTGCTTCGCA AGGTGGACTC AGACCTGAAC 
CAGAAATACA GCCCTGACTT CAAGGACCGA TACCAGATTG AGCTGCTGCT GCGGGAGCTG 
GATGACCAGG AGAAGGCTCT GGACAAGTAC CAGCATGTGG TGCGGGGGCT GCAGAAGCGA 
GGGCAGCAGG TGGTGCCCCT CAAGTACCGC CGGGAGACGC CCCTCAAGCC CATCCCCGTG 
GAGGCGCTCT GTGACTTCGA GGGTGACCAG GGCCTGATCT CTCGGGGCTA CAGCTACACC 
CTGCAGAGGA ACAATGGGGA GAGCTGGGAC CTCACCGACA GTGCGGGGAA CAAGCTGACC 
GCCCCGGCCG TCTGCTTCAT GATCCCGCCC ACTGACCCCG AGGCCCTGGC TCTGGCTGAA 
AGCCTGGGCA GCCAGTACCA GAGCCTGCGG CAGAAGGCAG CTGGGAGCAG AAAGGCGCTC 
CAGGAGCAGC ATGAGGTGCT GAAGGCAGAG AGCTCTGGAG ATGCCTCGGA CCTGCAGGGC 
CGCCAGCTGC TGGCCGGCTT GGACAAGGTG GCCAGCGACC TGGACCGGCA GGAGAAGGCC 
ATCACGGCCA TCCTGCGGCC GCCGCTGGAG CAGGGCCGGG CTGTGCAGGA CAGCGCCGAG 
AGGGCCAAGG ACCTCAAGAA CATCACCAAC GAGCTGCTGC GGATCGAGCC CGAGAAGGCG 
CGCAGCACGG CCGAGGGCGA GGCGTTCGTG CGGGCCCTCC CGGACAGCGG CAGCACCGCC 
CTGCTGAGGA CGCGGGTGGA GGACACCCAG CGCAGATACG AGCGCCTGGT GCAGCTGCTG 
GAAGCCGCCC AGGAGAAGCT CGACATCGCC AACCGCCTGG AGAAGAGCCT GCAGCAGGGC 
CGGGAGATGC TGGCCACGTA TGAGACCCAG CTGGCCCAGG AGGACACGGT GCCCGAGAGT 
GGCCAGGCCC TGGACAGCAG GAGGCAGGAG CTGGCGGCCA TGGCCTCCGA GCTGCAGGCC 
CGGAAGGCCC TCCTCGGCGA GGTGCAGCGG AACCTGCAGG CCGCCAAGCA GTGCTCGGGC 
TCGCTGGCCA GCCGCTTCCA GGAGCACTGC CCGGACCTGG AGCGCCAGGA GGCCGAGGTG 
CACAAGCTGT GCCAGCGCTT CGACGGCCTC TGCCAGCAGG CGGAGCTCAG GGTCCAGAGC 
CTGCAGAGCG CCCGGGCTGA GCACGACGCC TTCTGCAGCG GCCGTGACCG CCTGCTTCAG 
TTCCTGTCCC ACATCCCCAG CTACGAGCCC CAGGAGACGG ACGGCCTCGG CCAAGTGGAG 
ACCAAGCTGA ACAACCAGAA GAACCTACTA GATGAAATAG CAAGAAGGGA AGAGGAAGTG 
CAGAAAGTCC ACACGCACTC CCAGCAGTAC CAGCAGGCTG TCAAGGACTA CGAGTTGGAA 
GCAGAGAAAC TCAGGTCCCT CCTTGACTTG GAGAATGGAA GGAGCAGCCA CGTGAGCAAG 
CGGGCCAGGC TCCAGTCCCC TGCCACCAAA GTGAGGGAAG AGGAAGCAGC TCTTGCTGCC 
AAGTTTACGG AGGTTAATGC CATCAACAGA CAGAGACTGC AGAACCTGGA GTTTGCACTG 
ACCCTCCTGA GACAGCAGCT GGAGGCAGGT GCAAGCCAGG AGGCCCTGCC CGGGAGCAAG 
CCAGGCCCTG GAGCAGAGGA GACGTGGAAG ATTCAGGAGG AGCTGGATGA GGAGATGGAG 
CGGCGGCAGC AGCTGGAGCA CGAGGTCCGG AGCGCCCAGG AGGAGATCCG GGCCCTGCGG 
AGCCAGAGCC CGCAGGAGGC GGTGGTGACC AGGGAGGTGC TCAGGAAGGT GCCGGACCCA 
GCACTGGAGG AGAGCTTCCA GCAGGCGCAG CAGACCCTGG CCGAGGAGCA GCACAAGAAC 
CGGCTGCTGC AGGGGGAGGT GGAGGCGCTG CGGCTGCGGC TGCGTGCCCT GGAGGAGGCG 
GCCCGGGCCG GGGGCCAGGA GTACGTGGTC AAGGAGGTAC TGCGCATTGA GCCGGATCGG 
GCCCAGGCTG ACGAGGTCCT GAGGCTGCGG GGGGAGCTGG AGGTGCTGCG GCGGCAGAAG 
GGCGCCCGCG AGGCGGAGGC GCTTCTCCTG CAGCAGCGCG TGGCCGCCCT GGCCGAGGAG 
AAGCGGCGGG CGCGGGAGGT GGTCACAGAG AAGGAGGTGG TCAAGGTGCA CAATGACCCC 
CAGCTGGAGG CCGAGTTCCG GCAGCTGCAG GAGGACCAGC AGCGGGAGCA GGGGCTCCGG 
GAGAAGCAGG AGGAGGAGCT GGGCTTCCTG CAGGACAAGC TCAAGAGGCT GGAGAAGGAA 
CGGGCCATGG CCGAGGGCAA GATCACAGTC AAGGAGGTGC TCAAGGTGGA GAAGGACGTG 
GCGGCTGAGC GGGAGGTGGG CGCCCTCCAG CGCCGGTATG AGGATGAGGC CGCCAGGGCC 
CGTGCCAGCC AGAGGGAGAA GACCGAGCTG CTCCGGAAGA TCTGGGCCCT GGAGGAGGAG 
AACGCTAAGG TGGTTGTGCA AGAGAAGGTG CGCGAGATTG TCCGGCCTGA CCCCAAGGCT 
GAGAGTGAGG TGGCCAACCT CCGCCTGGAG CTGGTAGAGC AGGAGCGCAA GTACCGGGGG 
GCTGAGGAGC AGCTCAAGAG CTACCAGAGC GAGCTGGAGG CGCTTCGGAG GCGAGGCCCC 
CAGGTAGAGG TCAAGGAGGT GACCAAGGAG GTCATCAAGT ACACGACGGA CCCTGAGACG 
GAGAAGGAGC TCCAGAGGCT CAGGGAGGAG ATCGTGGACA AGACCCGCCT CATCGAGAGG 
TGTGACCTGG AGATCTACCA GCTGAAGCAG GAGATCCAGT CCCTCAAAGA GGCCAAGCCC 
CAGGTGCAGA CCAAGGAGGT GGTCCAGGAG ATCTTGCAGT TCCAGGAAGA CCCCCAGACC 
AAAGAGGAAG TGGCGGCTCT GAGGGCCCGG CTGGCTGAGG AGCACAAGAA GCAGGTAGAC 
CTGGAACGGG AATGCGTGTC ACAGGAGGAG AAGATCCAGG AGAAGGAGGA GGAGCTGGCC 
CGCGGGAAGG AGGTGGTGGT GCAGCAGGAG GTGGTCAGGT ACGAGGAGGA GCCGGGCCTC 
CGGGCGGAAG TGAGCGCCTT CACCGAGAGC ATCGATGCAG AACTGCGGCA GATCGACGGC 
CTCCGCGGCG AGCTGCGGCG GCTGCAGCGC CGGCGAGCCG AGCTGGAGCA CCAGCTGGAG 
GAGCTGGAGC GCGAGCGGCA GGCGCGCCGC GAGGCTGAGC TGGAGGTGCA GCACCTCAAG 
CAACAGCTGG CCCAGCTGGA GGCGCAGGAG GGCGAGGCCC ACGAGAAGGT GACCCTCACA 
CAGAAGGTGG TGCTGCAGCA GGACCCCCAG CAGGCCCGGG AGCATGCCCT CCTGGCCCTG 
CAGCTGGAGG AAGAGCGGCA CCGCCGGCAG GGCCTGGAGC GCGAGCTCGA GACCCTAAAG 
CAGCAGCTGG AGCACCTGGA GAAGATGGAG GTCAAGGAGA AGGTGGTCTT CTCCGAGAGC 
GTCCAGGTGG AGAAAGGCGA CACCGAGCAG GAGATTGAGA GGCTCAGGGG CAGTCTGGAG 
GCTGAGAGCC GAGGCAAGAG GGAGCTGGAC GCCGAGGTGA GCCGGCTGGA GGCCAAGCTG 
TCGGAGCTGG AGTTCTGCAA CTCTAAGTCA TCCAAGGAGC TGGACTTCCT GAGGGAGGAG 
AACCACAAGC TGCAGCTGGA GCGGCAGAGC CTGCAGCTCG AGGCCCGCAG GCTGCAGTCG 
GAGATCGAGA TGGCCAAGGC CGAGGCGCAG AGCCTAAGAA GCCTGCCCAC AACGACTGGA 
GCCGACCCCG GGGCGCAGCT CGACTCACGC CTGCGGTCCC TGGAGCGGGA GCTGGACGAC 
CTCAGGAGGC TCTCCAGGGA CAAAGACCAG GAGATCGAAG AGCTGCAGCG TCGCCTGGGG 
TCCGTGGCCA TCAAGCGGGA GCAGAGGGAG AACCACCTGC GGCGCTCCAT TGTGGTCATC 
GACCCTGACT CGGGCCGTGA GCTGTCCCCC GAGGAGGCCC ACCGCGCCGG CCTCATCGAC 
TGGAACATGT TTGTCAAGCT CAGGAGCCAG GAGTGCGACT GGGAGGAGAT CTCCGTCAAG 
GGCCCCGGAG GCGAGTCCTC CGTGATCCAT GACAGGAAGT CCGGCAGGAA GTTCTCCATT 
GAAGAGGCCC TGCAGAGCGG GCGGCTGACC CCCGCGCAGT ACGACCGCTA CGTCAACAAG 
AGCATGTCCA TCCAGGAGCT GGCCGTCCTG GTGTCAGGGC AGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPL ( XM_002697888.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002697934.3 Bos taurus periplakin (PPL), transcript variant X2, mRNA.

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CloneID	OBa32913
Clone ID Related Accession (Same CDS sequence)	XM_015469294.1 , XM_002697888.5 , XM_002697888.6
Accession Version	XM_002697888.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	periplakin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002697888.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCCACGGT TCGGACCCGC 
AGCGTCTCTA ACAAGGAGCT GTCGGAGCTC ATCGAGCAGT TGCAGAAGAA TGCCGACCAG 
GTGGAGAGGA ACATCGTGGA CACGGAAGCC AAGATGCAGA GAGACCTGGC CCGGCTGCAG 
GAGGGCCAGC AGCCCGAGCA CCGGGATGTG GCCCTGCAGA AGGTGTCAGA CTCTGAGAAG 
CTGCTCTACA TGCTGGAGGC GGACGCGGCC ATCGCCAAGC ACATGAAGCA CCCCCAGGGG 
GACATGATCG CCGAGGACAT CCGTCAGCTG AAGGAGCGTG TGACCAACCT CCGCGGGAAA 
CACAAGCAGA TCTACAACCT GGTGGTGAAA GAGGTGGACC CACAGGTCAA CTGGGAGGCG 
CTGGTGGAGG AAAAGCTGGA CGAGCTGAGC AGCCAGGGCT TCGGGACGGA ACTCCCGCTG 
GTAGGCCAGC AGGTGGAGCA ACACAACGTC TTTCACAACG AGGTCAAGGC CATCGGGCCC 
CACCTGGCCA AGGAGCAGAA CGGTGAACTC CAGGCCAAGT ACCAGAAACT GCTGGCGGCG 
TCACAGGCGC GGCAGCAGCA CCTGAGCTCA CTGCAGGACT ACATGCAGCG GTGCACGAAC 
GAGCTGTACT GGCTGGACCA GCAGGCCCAG GGCCGCATAC AGTATGACTG GAGTGACCGC 
AACCTCAACT ACCCCAGCCG CCGGCGCCAG TACGAGAATT TCATCCACCG GAACCTGGAG 
GCCAAAGAGG AGAGAATTAA CAAGCTGCAC AGCGAGGGTG ACCAGCTGCT CGCCGCCGAG 
CACCCCGGGA GGAACTCCAT TGAAGCTCAC ATGGAGGCTG TCCACGCAGA TTGGAAGGAA 
TACCTGAACC TGCTCATCTG CGAGGAGAGC CACCTGAAGT ACATGGAAGA CTACCACCAG 
TTCCACAAAG ACATGAAGGA TGCCCAGGAG CTGCTTCGCA AGGTGGACTC AGACCTGAAC 
CAGAAATACA GCCCTGACTT CAAGGACCGA TACCAGATTG AGCTGCTGCT GCGGGAGCTG 
GATGACCAGG AGAAGGCTCT GGACAAGTAC CAGCATGTGG TGCGGGGGCT GCAGAAGCGA 
GGGCAGCAGG TGGTGCCCCT CAAGTACCGC CGGGAGACGC CCCTCAAGCC CATCCCCGTG 
GAGGCGCTCT GTGACTTCGA GGGTGACCAG GGCCTGATCT CTCGGGGCTA CAGCTACACC 
CTGCAGAGGA ACAATGGGGA GAGCTGGGAC CTCACCGACA GTGCGGGGAA CAAGCTGACC 
GCCCCGGCCG TCTGCTTCAT GATCCCGCCC ACTGACCCCG AGGCCCTGGC TCTGGCTGAA 
AGCCTGGGCA GCCAGTACCA GAGCCTGCGG CAGAAGGCAG CTGGGAGCAG AAAGGCGCTC 
CAGGAGCAGC ATGAGGTGCT GAAGGCAGAG AGCTCTGGAG ATGCCTCGGA CCTGCAGGGC 
CGCCAGCTGC TGGCCGGCTT GGACAAGGTG GCCAGCGACC TGGACCGGCA GGAGAAGGCC 
ATCACGGCCA TCCTGCGGCC GCCGCTGGAG CAGGGCCGGG CTGTGCAGGA CAGCGCCGAG 
AGGGCCAAGG ACCTCAAGAA CATCACCAAC GAGCTGCTGC GGATCGAGCC CGAGAAGGCG 
CGCAGCACGG CCGAGGGCGA GGCGTTCGTG CGGGCCCTCC CGGACAGCGG CAGCACCGCC 
CTGCTGAGGA CGCGGGTGGA GGACACCCAG CGCAGATACG AGCGCCTGGT GCAGCTGCTG 
GAAGCCGCCC AGGAGAAGCT CGACATCGCC AACCGCCTGG AGAAGAGCCT GCAGCAGGGC 
CGGGAGATGC TGGCCACGTA TGAGACCCAG CTGGCCCAGG AGGACACGGT GCCCGAGAGT 
GGCCAGGCCC TGGACAGCAG GAGGCAGGAG CTGGCGGCCA TGGCCTCCGA GCTGCAGGCC 
CGGAAGGCCC TCCTCGGCGA GGTGCAGCGG AACCTGCAGG CCGCCAAGCA GTGCTCGGGC 
TCGCTGGCCA GCCGCTTCCA GGAGCACTGC CCGGACCTGG AGCGCCAGGA GGCCGAGGTG 
CACAAGCTGT GCCAGCGCTT CGACGGCCTC TGCCAGCAGG CGGAGCTCAG GGTCCAGAGC 
CTGCAGAGCG CCCGGGCTGA GCACGACGCC TTCTGCAGCG GCCGTGACCG CCTGCTTCAG 
TTCCTGTCCC ACATCCCCAG CTACGAGCCC CAGGAGACGG ACGGCCTCGG CCAAGTGGAG 
ACCAAGCTGA ACAACCAGAA GAACCTACTA GATGAAATAG CAAGAAGGGA AGAGGAAGTG 
CAGAAAGTCC ACACGCACTC CCAGCAGTAC CAGCAGGCTG TCAAGGACTA CGAGTTGGAA 
GCAGAGAAAC TCAGGTCCCT CCTTGACTTG GAGAATGGAA GGAGCAGCCA CGTGAGCAAG 
CGGGCCAGGC TCCAGTCCCC TGCCACCAAA GTGAGGGAAG AGGAAGCAGC TCTTGCTGCC 
AAGTTTACGG AGGTTAATGC CATCAACAGA CAGAGACTGC AGAACCTGGA GTTTGCACTG 
ACCCTCCTGA GACAGCAGCT GGAGGCAGGT GCAAGCCAGG AGGCCCTGCC CGGGAGCAAG 
CCAGGCCCTG GAGCAGAGGA GACGTGGAAG ATTCAGGAGG AGCTGGATGA GGAGATGGAG 
CGGCGGCAGC AGCTGGAGCA CGAGGTCCGG AGCGCCCAGG AGGAGATCCG GGCCCTGCGG 
AGCCAGAGCC CGCAGGAGGC GGTGGTGACC AGGGAGGTGC TCAGGAAGGT GCCGGACCCA 
GCACTGGAGG AGAGCTTCCA GCAGGCGCAG CAGACCCTGG CCGAGGAGCA GCACAAGAAC 
CGGCTGCTGC AGGGGGAGGT GGAGGCGCTG CGGCTGCGGC TGCGTGCCCT GGAGGAGGCG 
GCCCGGGCCG GGGGCCAGGA GTACGTGGTC AAGGAGGTAC TGCGCATTGA GCCGGATCGG 
GCCCAGGCTG ACGAGGTCCT GAGGCTGCGG GGGGAGCTGG AGGTGCTGCG GCGGCAGAAG 
GGCGCCCGCG AGGCGGAGGC GCTTCTCCTG CAGCAGCGCG TGGCCGCCCT GGCCGAGGAG 
AAGCGGCGGG CGCGGGAGGT GGTCACAGAG AAGGAGGTGG TCAAGGTGCA CAATGACCCC 
CAGCTGGAGG CCGAGTTCCG GCAGCTGCAG GAGGACCAGC AGCGGGAGCA GGGGCTCCGG 
GAGAAGCAGG AGGAGGAGCT GGGCTTCCTG CAGGACAAGC TCAAGAGGCT GGAGAAGGAA 
CGGGCCATGG CCGAGGGCAA GATCACAGTC AAGGAGGTGC TCAAGGTGGA GAAGGACGTG 
GCGGCTGAGC GGGAGGTGGG CGCCCTCCAG CGCCGGTATG AGGATGAGGC CGCCAGGGCC 
CGTGCCAGCC AGAGGGAGAA GACCGAGCTG CTCCGGAAGA TCTGGGCCCT GGAGGAGGAG 
AACGCTAAGG TGGTTGTGCA AGAGAAGGTG CGCGAGATTG TCCGGCCTGA CCCCAAGGCT 
GAGAGTGAGG TGGCCAACCT CCGCCTGGAG CTGGTAGAGC AGGAGCGCAA GTACCGGGGG 
GCTGAGGAGC AGCTCAAGAG CTACCAGAGC GAGCTGGAGG CGCTTCGGAG GCGAGGCCCC 
CAGGTAGAGG TCAAGGAGGT GACCAAGGAG GTCATCAAGT ACACGACGGA CCCTGAGACG 
GAGAAGGAGC TCCAGAGGCT CAGGGAGGAG ATCGTGGACA AGACCCGCCT CATCGAGAGG 
TGTGACCTGG AGATCTACCA GCTGAAGCAG GAGATCCAGT CCCTCAAAGA GGCCAAGCCC 
CAGGTGCAGA CCAAGGAGGT GGTCCAGGAG ATCTTGCAGT TCCAGGAAGA CCCCCAGACC 
AAAGAGGAAG TGGCGGCTCT GAGGGCCCGG CTGGCTGAGG AGCACAAGAA GCAGGTAGAC 
CTGGAACGGG AATGCGTGTC ACAGGAGGAG AAGATCCAGG AGAAGGAGGA GGAGCTGGCC 
CGCGGGAAGG AGGTGGTGGT GCAGCAGGAG GTGGTCAGGT ACGAGGAGGA GCCGGGCCTC 
CGGGCGGAAG TGAGCGCCTT CACCGAGAGC ATCGATGCAG AACTGCGGCA GATCGACGGC 
CTCCGCGGCG AGCTGCGGCG GCTGCAGCGC CGGCGAGCCG AGCTGGAGCA CCAGCTGGAG 
GAGCTGGAGC GCGAGCGGCA GGCGCGCCGC GAGGCTGAGC TGGAGGTGCA GCACCTCAAG 
CAACAGCTGG CCCAGCTGGA GGCGCAGGAG GGCGAGGCCC ACGAGAAGGT GACCCTCACA 
CAGAAGGTGG TGCTGCAGCA GGACCCCCAG CAGGCCCGGG AGCATGCCCT CCTGGCCCTG 
CAGCTGGAGG AAGAGCGGCA CCGCCGGCAG GGCCTGGAGC GCGAGCTCGA GACCCTAAAG 
CAGCAGCTGG AGCACCTGGA GAAGATGGAG GTCAAGGAGA AGGTGGTCTT CTCCGAGAGC 
GTCCAGGTGG AGAAAGGCGA CACCGAGCAG GAGATTGAGA GGCTCAGGGG CAGTCTGGAG 
GCTGAGAGCC GAGGCAAGAG GGAGCTGGAC GCCGAGGTGA GCCGGCTGGA GGCCAAGCTG 
TCGGAGCTGG AGTTCTGCAA CTCTAAGTCA TCCAAGGAGC TGGACTTCCT GAGGGAGGAG 
AACCACAAGC TGCAGCTGGA GCGGCAGAGC CTGCAGCTCG AGGCCCGCAG GCTGCAGTCG 
GAGATCGAGA TGGCCAAGGC CGAGGCGCAG AGCCTAAGAA GCCTGCCCAC AACGACTGGA 
GCCGACCCCG GGGCGCAGCT CGACTCACGC CTGCGGTCCC TGGAGCGGGA GCTGGACGAC 
CTCAGGAGGC TCTCCAGGGA CAAAGACCAG GAGATCGAAG AGCTGCAGCG TCGCCTGGGG 
TCCGTGGCCA TCAAGCGGGA GCAGAGGGAG AACCACCTGC GGCGCTCCAT TGTGGTCATC 
GACCCTGACT CGGGCCGTGA GCTGTCCCCC GAGGAGGCCC ACCGCGCCGG CCTCATCGAC 
TGGAACATGT TTGTCAAGCT CAGGAGCCAG GAGTGCGACT GGGAGGAGAT CTCCGTCAAG 
GGCCCCGGAG GCGAGTCCTC CGTGATCCAT GACAGGAAGT CCGGCAGGAA GTTCTCCATT 
GAAGAGGCCC TGCAGAGCGG GCGGCTGACC CCCGCGCAGT ACGACCGCTA CGTCAACAAG 
AGCATGTCCA TCCAGGAGCT GGCCGTCCTG GTGTCAGGGC AGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002697934.3
CDS	207..5474
Translation	MNSLFRKRNKGKYSPTVRTRSVSNKELSELIEQLQKNADQVERNIVDTEAKMQRDLARLQEGQQPEHRDVALQKVSDSEKLLYMLEADAAIAKHMKHPQGDMIAEDIRQLKERVTNLRGKHKQIYNLVVKEVDPQVNWEALVEEKLDELSSQGFGTELPLVGQQVEQHNVFHNEVKAIGPHLAKEQNGELQAKYQKLLAASQARQQHLSSLQDYMQRCTNELYWLDQQAQGRIQYDWSDRNLNYPSRRRQYENFIHRNLEAKEERINKLHSEGDQLLAAEHPGRNSIEAHMEAVHADWKEYLNLLICEESHLKYMEDYHQFHKDMKDAQELLRKVDSDLNQKYSPDFKDRYQIELLLRELDDQEKALDKYQHVVRGLQKRGQQVVPLKYRRETPLKPIPVEALCDFEGDQGLISRGYSYTLQRNNGESWDLTDSAGNKLTAPAVCFMIPPTDPEALALAESLGSQYQSLRQKAAGSRKALQEQHEVLKAESSGDASDLQGRQLLAGLDKVASDLDRQEKAITAILRPPLEQGRAVQDSAERAKDLKNITNELLRIEPEKARSTAEGEAFVRALPDSGSTALLRTRVEDTQRRYERLVQLLEAAQEKLDIANRLEKSLQQGREMLATYETQLAQEDTVPESGQALDSRRQELAAMASELQARKALLGEVQRNLQAAKQCSGSLASRFQEHCPDLERQEAEVHKLCQRFDGLCQQAELRVQSLQSARAEHDAFCSGRDRLLQFLSHIPSYEPQETDGLGQVETKLNNQKNLLDEIARREEEVQKVHTHSQQYQQAVKDYELEAEKLRSLLDLENGRSSHVSKRARLQSPATKVREEEAALAAKFTEVNAINRQRLQNLEFALTLLRQQLEAGASQEALPGSKPGPGAEETWKIQEELDEEMERRQQLEHEVRSAQEEIRALRSQSPQEAVVTREVLRKVPDPALEESFQQAQQTLAEEQHKNRLLQGEVEALRLRLRALEEAARAGGQEYVVKEVLRIEPDRAQADEVLRLRGELEVLRRQKGAREAEALLLQQRVAALAEEKRRAREVVTEKEVVKVHNDPQLEAEFRQLQEDQQREQGLREKQEEELGFLQDKLKRLEKERAMAEGKITVKEVLKVEKDVAAEREVGALQRRYEDEAARARASQREKTELLRKIWALEEENAKVVVQEKVREIVRPDPKAESEVANLRLELVEQERKYRGAEEQLKSYQSELEALRRRGPQVEVKEVTKEVIKYTTDPETEKELQRLREEIVDKTRLIERCDLEIYQLKQEIQSLKEAKPQVQTKEVVQEILQFQEDPQTKEEVAALRARLAEEHKKQVDLERECVSQEEKIQEKEEELARGKEVVVQQEVVRYEEEPGLRAEVSAFTESIDAELRQIDGLRGELRRLQRRRAELEHQLEELERERQARREAELEVQHLKQQLAQLEAQEGEAHEKVTLTQKVVLQQDPQQAREHALLALQLEEERHRRQGLERELETLKQQLEHLEKMEVKEKVVFSESVQVEKGDTEQEIERLRGSLEAESRGKRELDAEVSRLEAKLSELEFCNSKSSKELDFLREENHKLQLERQSLQLEARRLQSEIEMAKAEAQSLRSLPTTTGADPGAQLDSRLRSLERELDDLRRLSRDKDQEIEELQRRLGSVAIKREQRENHLRRSIVVIDPDSGRELSPEEAHRAGLIDWNMFVKLRSQECDWEEISVKGPGGESSVIHDRKSGRKFSIEEALQSGRLTPAQYDRYVNKSMSIQELAVLVSGQK

Target ORF information:

RefSeq Version	XM_002697888.5
Organism	Bos taurus(cattle)
Definition	Bos taurus periplakin (PPL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002697888.5

ORF Insert Sequence:

ATGAACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCCACGGT TCGGACCCGC 
AGCGTCTCTA ACAAGGAGCT GTCGGAGCTC ATCGAGCAGT TGCAGAAGAA TGCCGACCAG 
GTGGAGAGGA ACATCGTGGA CACGGAAGCC AAGATGCAGA GAGACCTGGC CCGGCTGCAG 
GAGGGCCAGC AGCCCGAGCA CCGGGATGTG GCCCTGCAGA AGGTGTCAGA CTCTGAGAAG 
CTGCTCTACA TGCTGGAGGC GGACGCGGCC ATCGCCAAGC ACATGAAGCA CCCCCAGGGG 
GACATGATCG CCGAGGACAT CCGTCAGCTG AAGGAGCGTG TGACCAACCT CCGCGGGAAA 
CACAAGCAGA TCTACAACCT GGTGGTGAAA GAGGTGGACC CACAGGTCAA CTGGGAGGCG 
CTGGTGGAGG AAAAGCTGGA CGAGCTGAGC AGCCAGGGCT TCGGGACGGA ACTCCCGCTG 
GTAGGCCAGC AGGTGGAGCA ACACAACGTC TTTCACAACG AGGTCAAGGC CATCGGGCCC 
CACCTGGCCA AGGAGCAGAA CGGTGAACTC CAGGCCAAGT ACCAGAAACT GCTGGCGGCG 
TCACAGGCGC GGCAGCAGCA CCTGAGCTCA CTGCAGGACT ACATGCAGCG GTGCACGAAC 
GAGCTGTACT GGCTGGACCA GCAGGCCCAG GGCCGCATAC AGTATGACTG GAGTGACCGC 
AACCTCAACT ACCCCAGCCG CCGGCGCCAG TACGAGAATT TCATCCACCG GAACCTGGAG 
GCCAAAGAGG AGAGAATTAA CAAGCTGCAC AGCGAGGGTG ACCAGCTGCT CGCCGCCGAG 
CACCCCGGGA GGAACTCCAT TGAAGCTCAC ATGGAGGCTG TCCACGCAGA TTGGAAGGAA 
TACCTGAACC TGCTCATCTG CGAGGAGAGC CACCTGAAGT ACATGGAAGA CTACCACCAG 
TTCCACAAAG ACATGAAGGA TGCCCAGGAG CTGCTTCGCA AGGTGGACTC AGACCTGAAC 
CAGAAATACA GCCCTGACTT CAAGGACCGA TACCAGATTG AGCTGCTGCT GCGGGAGCTG 
GATGACCAGG AGAAGGCTCT GGACAAGTAC CAGCATGTGG TGCGGGGGCT GCAGAAGCGA 
GGGCAGCAGG TGGTGCCCCT CAAGTACCGC CGGGAGACGC CCCTCAAGCC CATCCCCGTG 
GAGGCGCTCT GTGACTTCGA GGGTGACCAG GGCCTGATCT CTCGGGGCTA CAGCTACACC 
CTGCAGAGGA ACAATGGGGA GAGCTGGGAC CTCACCGACA GTGCGGGGAA CAAGCTGACC 
GCCCCGGCCG TCTGCTTCAT GATCCCGCCC ACTGACCCCG AGGCCCTGGC TCTGGCTGAA 
AGCCTGGGCA GCCAGTACCA GAGCCTGCGG CAGAAGGCAG CTGGGAGCAG AAAGGCGCTC 
CAGGAGCAGC ATGAGGTGCT GAAGGCAGAG AGCTCTGGAG ATGCCTCGGA CCTGCAGGGC 
CGCCAGCTGC TGGCCGGCTT GGACAAGGTG GCCAGCGACC TGGACCGGCA GGAGAAGGCC 
ATCACGGCCA TCCTGCGGCC GCCGCTGGAG CAGGGCCGGG CTGTGCAGGA CAGCGCCGAG 
AGGGCCAAGG ACCTCAAGAA CATCACCAAC GAGCTGCTGC GGATCGAGCC CGAGAAGGCG 
CGCAGCACGG CCGAGGGCGA GGCGTTCGTG CGGGCCCTCC CGGACAGCGG CAGCACCGCC 
CTGCTGAGGA CGCGGGTGGA GGACACCCAG CGCAGATACG AGCGCCTGGT GCAGCTGCTG 
GAAGCCGCCC AGGAGAAGCT CGACATCGCC AACCGCCTGG AGAAGAGCCT GCAGCAGGGC 
CGGGAGATGC TGGCCACGTA TGAGACCCAG CTGGCCCAGG AGGACACGGT GCCCGAGAGT 
GGCCAGGCCC TGGACAGCAG GAGGCAGGAG CTGGCGGCCA TGGCCTCCGA GCTGCAGGCC 
CGGAAGGCCC TCCTCGGCGA GGTGCAGCGG AACCTGCAGG CCGCCAAGCA GTGCTCGGGC 
TCGCTGGCCA GCCGCTTCCA GGAGCACTGC CCGGACCTGG AGCGCCAGGA GGCCGAGGTG 
CACAAGCTGT GCCAGCGCTT CGACGGCCTC TGCCAGCAGG CGGAGCTCAG GGTCCAGAGC 
CTGCAGAGCG CCCGGGCTGA GCACGACGCC TTCTGCAGCG GCCGTGACCG CCTGCTTCAG 
TTCCTGTCCC ACATCCCCAG CTACGAGCCC CAGGAGACGG ACGGCCTCGG CCAAGTGGAG 
ACCAAGCTGA ACAACCAGAA GAACCTACTA GATGAAATAG CAAGAAGGGA AGAGGAAGTG 
CAGAAAGTCC ACACGCACTC CCAGCAGTAC CAGCAGGCTG TCAAGGACTA CGAGTTGGAA 
GCAGAGAAAC TCAGGTCCCT CCTTGACTTG GAGAATGGAA GGAGCAGCCA CGTGAGCAAG 
CGGGCCAGGC TCCAGTCCCC TGCCACCAAA GTGAGGGAAG AGGAAGCAGC TCTTGCTGCC 
AAGTTTACGG AGGTTAATGC CATCAACAGA CAGAGACTGC AGAACCTGGA GTTTGCACTG 
ACCCTCCTGA GACAGCAGCT GGAGGCAGGT GCAAGCCAGG AGGCCCTGCC CGGGAGCAAG 
CCAGGCCCTG GAGCAGAGGA GACGTGGAAG ATTCAGGAGG AGCTGGATGA GGAGATGGAG 
CGGCGGCAGC AGCTGGAGCA CGAGGTCCGG AGCGCCCAGG AGGAGATCCG GGCCCTGCGG 
AGCCAGAGCC CGCAGGAGGC GGTGGTGACC AGGGAGGTGC TCAGGAAGGT GCCGGACCCA 
GCACTGGAGG AGAGCTTCCA GCAGGCGCAG CAGACCCTGG CCGAGGAGCA GCACAAGAAC 
CGGCTGCTGC AGGGGGAGGT GGAGGCGCTG CGGCTGCGGC TGCGTGCCCT GGAGGAGGCG 
GCCCGGGCCG GGGGCCAGGA GTACGTGGTC AAGGAGGTAC TGCGCATTGA GCCGGATCGG 
GCCCAGGCTG ACGAGGTCCT GAGGCTGCGG GGGGAGCTGG AGGTGCTGCG GCGGCAGAAG 
GGCGCCCGCG AGGCGGAGGC GCTTCTCCTG CAGCAGCGCG TGGCCGCCCT GGCCGAGGAG 
AAGCGGCGGG CGCGGGAGGT GGTCACAGAG AAGGAGGTGG TCAAGGTGCA CAATGACCCC 
CAGCTGGAGG CCGAGTTCCG GCAGCTGCAG GAGGACCAGC AGCGGGAGCA GGGGCTCCGG 
GAGAAGCAGG AGGAGGAGCT GGGCTTCCTG CAGGACAAGC TCAAGAGGCT GGAGAAGGAA 
CGGGCCATGG CCGAGGGCAA GATCACAGTC AAGGAGGTGC TCAAGGTGGA GAAGGACGTG 
GCGGCTGAGC GGGAGGTGGG CGCCCTCCAG CGCCGGTATG AGGATGAGGC CGCCAGGGCC 
CGTGCCAGCC AGAGGGAGAA GACCGAGCTG CTCCGGAAGA TCTGGGCCCT GGAGGAGGAG 
AACGCTAAGG TGGTTGTGCA AGAGAAGGTG CGCGAGATTG TCCGGCCTGA CCCCAAGGCT 
GAGAGTGAGG TGGCCAACCT CCGCCTGGAG CTGGTAGAGC AGGAGCGCAA GTACCGGGGG 
GCTGAGGAGC AGCTCAAGAG CTACCAGAGC GAGCTGGAGG CGCTTCGGAG GCGAGGCCCC 
CAGGTAGAGG TCAAGGAGGT GACCAAGGAG GTCATCAAGT ACACGACGGA CCCTGAGACG 
GAGAAGGAGC TCCAGAGGCT CAGGGAGGAG ATCGTGGACA AGACCCGCCT CATCGAGAGG 
TGTGACCTGG AGATCTACCA GCTGAAGCAG GAGATCCAGT CCCTCAAAGA GGCCAAGCCC 
CAGGTGCAGA CCAAGGAGGT GGTCCAGGAG ATCTTGCAGT TCCAGGAAGA CCCCCAGACC 
AAAGAGGAAG TGGCGGCTCT GAGGGCCCGG CTGGCTGAGG AGCACAAGAA GCAGGTAGAC 
CTGGAACGGG AATGCGTGTC ACAGGAGGAG AAGATCCAGG AGAAGGAGGA GGAGCTGGCC 
CGCGGGAAGG AGGTGGTGGT GCAGCAGGAG GTGGTCAGGT ACGAGGAGGA GCCGGGCCTC 
CGGGCGGAAG TGAGCGCCTT CACCGAGAGC ATCGATGCAG AACTGCGGCA GATCGACGGC 
CTCCGCGGCG AGCTGCGGCG GCTGCAGCGC CGGCGAGCCG AGCTGGAGCA CCAGCTGGAG 
GAGCTGGAGC GCGAGCGGCA GGCGCGCCGC GAGGCTGAGC TGGAGGTGCA GCACCTCAAG 
CAACAGCTGG CCCAGCTGGA GGCGCAGGAG GGCGAGGCCC ACGAGAAGGT GACCCTCACA 
CAGAAGGTGG TGCTGCAGCA GGACCCCCAG CAGGCCCGGG AGCATGCCCT CCTGGCCCTG 
CAGCTGGAGG AAGAGCGGCA CCGCCGGCAG GGCCTGGAGC GCGAGCTCGA GACCCTAAAG 
CAGCAGCTGG AGCACCTGGA GAAGATGGAG GTCAAGGAGA AGGTGGTCTT CTCCGAGAGC 
GTCCAGGTGG AGAAAGGCGA CACCGAGCAG GAGATTGAGA GGCTCAGGGG CAGTCTGGAG 
GCTGAGAGCC GAGGCAAGAG GGAGCTGGAC GCCGAGGTGA GCCGGCTGGA GGCCAAGCTG 
TCGGAGCTGG AGTTCTGCAA CTCTAAGTCA TCCAAGGAGC TGGACTTCCT GAGGGAGGAG 
AACCACAAGC TGCAGCTGGA GCGGCAGAGC CTGCAGCTCG AGGCCCGCAG GCTGCAGTCG 
GAGATCGAGA TGGCCAAGGC CGAGGCGCAG AGCCTAAGAA GCCTGCCCAC AACGACTGGA 
GCCGACCCCG GGGCGCAGCT CGACTCACGC CTGCGGTCCC TGGAGCGGGA GCTGGACGAC 
CTCAGGAGGC TCTCCAGGGA CAAAGACCAG GAGATCGAAG AGCTGCAGCG TCGCCTGGGG 
TCCGTGGCCA TCAAGCGGGA GCAGAGGGAG AACCACCTGC GGCGCTCCAT TGTGGTCATC 
GACCCTGACT CGGGCCGTGA GCTGTCCCCC GAGGAGGCCC ACCGCGCCGG CCTCATCGAC 
TGGAACATGT TTGTCAAGCT CAGGAGCCAG GAGTGCGACT GGGAGGAGAT CTCCGTCAAG 
GGCCCCGGAG GCGAGTCCTC CGTGATCCAT GACAGGAAGT CCGGCAGGAA GTTCTCCATT 
GAAGAGGCCC TGCAGAGCGG GCGGCTGACC CCCGCGCAGT ACGACCGCTA CGTCAACAAG 
AGCATGTCCA TCCAGGAGCT GGCCGTCCTG GTGTCAGGGC AGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPL ( XM_002697888.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002697934.3 Bos taurus periplakin (PPL), transcript variant X2, mRNA.

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CloneID	OBa32913
Clone ID Related Accession (Same CDS sequence)	XM_015469294.1 , XM_002697888.5 , XM_002697888.6
Accession Version	XM_002697888.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	periplakin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002697888.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCCACGGT TCGGACCCGC 
AGCGTCTCTA ACAAGGAGCT GTCGGAGCTC ATCGAGCAGT TGCAGAAGAA TGCCGACCAG 
GTGGAGAGGA ACATCGTGGA CACGGAAGCC AAGATGCAGA GAGACCTGGC CCGGCTGCAG 
GAGGGCCAGC AGCCCGAGCA CCGGGATGTG GCCCTGCAGA AGGTGTCAGA CTCTGAGAAG 
CTGCTCTACA TGCTGGAGGC GGACGCGGCC ATCGCCAAGC ACATGAAGCA CCCCCAGGGG 
GACATGATCG CCGAGGACAT CCGTCAGCTG AAGGAGCGTG TGACCAACCT CCGCGGGAAA 
CACAAGCAGA TCTACAACCT GGTGGTGAAA GAGGTGGACC CACAGGTCAA CTGGGAGGCG 
CTGGTGGAGG AAAAGCTGGA CGAGCTGAGC AGCCAGGGCT TCGGGACGGA ACTCCCGCTG 
GTAGGCCAGC AGGTGGAGCA ACACAACGTC TTTCACAACG AGGTCAAGGC CATCGGGCCC 
CACCTGGCCA AGGAGCAGAA CGGTGAACTC CAGGCCAAGT ACCAGAAACT GCTGGCGGCG 
TCACAGGCGC GGCAGCAGCA CCTGAGCTCA CTGCAGGACT ACATGCAGCG GTGCACGAAC 
GAGCTGTACT GGCTGGACCA GCAGGCCCAG GGCCGCATAC AGTATGACTG GAGTGACCGC 
AACCTCAACT ACCCCAGCCG CCGGCGCCAG TACGAGAATT TCATCCACCG GAACCTGGAG 
GCCAAAGAGG AGAGAATTAA CAAGCTGCAC AGCGAGGGTG ACCAGCTGCT CGCCGCCGAG 
CACCCCGGGA GGAACTCCAT TGAAGCTCAC ATGGAGGCTG TCCACGCAGA TTGGAAGGAA 
TACCTGAACC TGCTCATCTG CGAGGAGAGC CACCTGAAGT ACATGGAAGA CTACCACCAG 
TTCCACAAAG ACATGAAGGA TGCCCAGGAG CTGCTTCGCA AGGTGGACTC AGACCTGAAC 
CAGAAATACA GCCCTGACTT CAAGGACCGA TACCAGATTG AGCTGCTGCT GCGGGAGCTG 
GATGACCAGG AGAAGGCTCT GGACAAGTAC CAGCATGTGG TGCGGGGGCT GCAGAAGCGA 
GGGCAGCAGG TGGTGCCCCT CAAGTACCGC CGGGAGACGC CCCTCAAGCC CATCCCCGTG 
GAGGCGCTCT GTGACTTCGA GGGTGACCAG GGCCTGATCT CTCGGGGCTA CAGCTACACC 
CTGCAGAGGA ACAATGGGGA GAGCTGGGAC CTCACCGACA GTGCGGGGAA CAAGCTGACC 
GCCCCGGCCG TCTGCTTCAT GATCCCGCCC ACTGACCCCG AGGCCCTGGC TCTGGCTGAA 
AGCCTGGGCA GCCAGTACCA GAGCCTGCGG CAGAAGGCAG CTGGGAGCAG AAAGGCGCTC 
CAGGAGCAGC ATGAGGTGCT GAAGGCAGAG AGCTCTGGAG ATGCCTCGGA CCTGCAGGGC 
CGCCAGCTGC TGGCCGGCTT GGACAAGGTG GCCAGCGACC TGGACCGGCA GGAGAAGGCC 
ATCACGGCCA TCCTGCGGCC GCCGCTGGAG CAGGGCCGGG CTGTGCAGGA CAGCGCCGAG 
AGGGCCAAGG ACCTCAAGAA CATCACCAAC GAGCTGCTGC GGATCGAGCC CGAGAAGGCG 
CGCAGCACGG CCGAGGGCGA GGCGTTCGTG CGGGCCCTCC CGGACAGCGG CAGCACCGCC 
CTGCTGAGGA CGCGGGTGGA GGACACCCAG CGCAGATACG AGCGCCTGGT GCAGCTGCTG 
GAAGCCGCCC AGGAGAAGCT CGACATCGCC AACCGCCTGG AGAAGAGCCT GCAGCAGGGC 
CGGGAGATGC TGGCCACGTA TGAGACCCAG CTGGCCCAGG AGGACACGGT GCCCGAGAGT 
GGCCAGGCCC TGGACAGCAG GAGGCAGGAG CTGGCGGCCA TGGCCTCCGA GCTGCAGGCC 
CGGAAGGCCC TCCTCGGCGA GGTGCAGCGG AACCTGCAGG CCGCCAAGCA GTGCTCGGGC 
TCGCTGGCCA GCCGCTTCCA GGAGCACTGC CCGGACCTGG AGCGCCAGGA GGCCGAGGTG 
CACAAGCTGT GCCAGCGCTT CGACGGCCTC TGCCAGCAGG CGGAGCTCAG GGTCCAGAGC 
CTGCAGAGCG CCCGGGCTGA GCACGACGCC TTCTGCAGCG GCCGTGACCG CCTGCTTCAG 
TTCCTGTCCC ACATCCCCAG CTACGAGCCC CAGGAGACGG ACGGCCTCGG CCAAGTGGAG 
ACCAAGCTGA ACAACCAGAA GAACCTACTA GATGAAATAG CAAGAAGGGA AGAGGAAGTG 
CAGAAAGTCC ACACGCACTC CCAGCAGTAC CAGCAGGCTG TCAAGGACTA CGAGTTGGAA 
GCAGAGAAAC TCAGGTCCCT CCTTGACTTG GAGAATGGAA GGAGCAGCCA CGTGAGCAAG 
CGGGCCAGGC TCCAGTCCCC TGCCACCAAA GTGAGGGAAG AGGAAGCAGC TCTTGCTGCC 
AAGTTTACGG AGGTTAATGC CATCAACAGA CAGAGACTGC AGAACCTGGA GTTTGCACTG 
ACCCTCCTGA GACAGCAGCT GGAGGCAGGT GCAAGCCAGG AGGCCCTGCC CGGGAGCAAG 
CCAGGCCCTG GAGCAGAGGA GACGTGGAAG ATTCAGGAGG AGCTGGATGA GGAGATGGAG 
CGGCGGCAGC AGCTGGAGCA CGAGGTCCGG AGCGCCCAGG AGGAGATCCG GGCCCTGCGG 
AGCCAGAGCC CGCAGGAGGC GGTGGTGACC AGGGAGGTGC TCAGGAAGGT GCCGGACCCA 
GCACTGGAGG AGAGCTTCCA GCAGGCGCAG CAGACCCTGG CCGAGGAGCA GCACAAGAAC 
CGGCTGCTGC AGGGGGAGGT GGAGGCGCTG CGGCTGCGGC TGCGTGCCCT GGAGGAGGCG 
GCCCGGGCCG GGGGCCAGGA GTACGTGGTC AAGGAGGTAC TGCGCATTGA GCCGGATCGG 
GCCCAGGCTG ACGAGGTCCT GAGGCTGCGG GGGGAGCTGG AGGTGCTGCG GCGGCAGAAG 
GGCGCCCGCG AGGCGGAGGC GCTTCTCCTG CAGCAGCGCG TGGCCGCCCT GGCCGAGGAG 
AAGCGGCGGG CGCGGGAGGT GGTCACAGAG AAGGAGGTGG TCAAGGTGCA CAATGACCCC 
CAGCTGGAGG CCGAGTTCCG GCAGCTGCAG GAGGACCAGC AGCGGGAGCA GGGGCTCCGG 
GAGAAGCAGG AGGAGGAGCT GGGCTTCCTG CAGGACAAGC TCAAGAGGCT GGAGAAGGAA 
CGGGCCATGG CCGAGGGCAA GATCACAGTC AAGGAGGTGC TCAAGGTGGA GAAGGACGTG 
GCGGCTGAGC GGGAGGTGGG CGCCCTCCAG CGCCGGTATG AGGATGAGGC CGCCAGGGCC 
CGTGCCAGCC AGAGGGAGAA GACCGAGCTG CTCCGGAAGA TCTGGGCCCT GGAGGAGGAG 
AACGCTAAGG TGGTTGTGCA AGAGAAGGTG CGCGAGATTG TCCGGCCTGA CCCCAAGGCT 
GAGAGTGAGG TGGCCAACCT CCGCCTGGAG CTGGTAGAGC AGGAGCGCAA GTACCGGGGG 
GCTGAGGAGC AGCTCAAGAG CTACCAGAGC GAGCTGGAGG CGCTTCGGAG GCGAGGCCCC 
CAGGTAGAGG TCAAGGAGGT GACCAAGGAG GTCATCAAGT ACACGACGGA CCCTGAGACG 
GAGAAGGAGC TCCAGAGGCT CAGGGAGGAG ATCGTGGACA AGACCCGCCT CATCGAGAGG 
TGTGACCTGG AGATCTACCA GCTGAAGCAG GAGATCCAGT CCCTCAAAGA GGCCAAGCCC 
CAGGTGCAGA CCAAGGAGGT GGTCCAGGAG ATCTTGCAGT TCCAGGAAGA CCCCCAGACC 
AAAGAGGAAG TGGCGGCTCT GAGGGCCCGG CTGGCTGAGG AGCACAAGAA GCAGGTAGAC 
CTGGAACGGG AATGCGTGTC ACAGGAGGAG AAGATCCAGG AGAAGGAGGA GGAGCTGGCC 
CGCGGGAAGG AGGTGGTGGT GCAGCAGGAG GTGGTCAGGT ACGAGGAGGA GCCGGGCCTC 
CGGGCGGAAG TGAGCGCCTT CACCGAGAGC ATCGATGCAG AACTGCGGCA GATCGACGGC 
CTCCGCGGCG AGCTGCGGCG GCTGCAGCGC CGGCGAGCCG AGCTGGAGCA CCAGCTGGAG 
GAGCTGGAGC GCGAGCGGCA GGCGCGCCGC GAGGCTGAGC TGGAGGTGCA GCACCTCAAG 
CAACAGCTGG CCCAGCTGGA GGCGCAGGAG GGCGAGGCCC ACGAGAAGGT GACCCTCACA 
CAGAAGGTGG TGCTGCAGCA GGACCCCCAG CAGGCCCGGG AGCATGCCCT CCTGGCCCTG 
CAGCTGGAGG AAGAGCGGCA CCGCCGGCAG GGCCTGGAGC GCGAGCTCGA GACCCTAAAG 
CAGCAGCTGG AGCACCTGGA GAAGATGGAG GTCAAGGAGA AGGTGGTCTT CTCCGAGAGC 
GTCCAGGTGG AGAAAGGCGA CACCGAGCAG GAGATTGAGA GGCTCAGGGG CAGTCTGGAG 
GCTGAGAGCC GAGGCAAGAG GGAGCTGGAC GCCGAGGTGA GCCGGCTGGA GGCCAAGCTG 
TCGGAGCTGG AGTTCTGCAA CTCTAAGTCA TCCAAGGAGC TGGACTTCCT GAGGGAGGAG 
AACCACAAGC TGCAGCTGGA GCGGCAGAGC CTGCAGCTCG AGGCCCGCAG GCTGCAGTCG 
GAGATCGAGA TGGCCAAGGC CGAGGCGCAG AGCCTAAGAA GCCTGCCCAC AACGACTGGA 
GCCGACCCCG GGGCGCAGCT CGACTCACGC CTGCGGTCCC TGGAGCGGGA GCTGGACGAC 
CTCAGGAGGC TCTCCAGGGA CAAAGACCAG GAGATCGAAG AGCTGCAGCG TCGCCTGGGG 
TCCGTGGCCA TCAAGCGGGA GCAGAGGGAG AACCACCTGC GGCGCTCCAT TGTGGTCATC 
GACCCTGACT CGGGCCGTGA GCTGTCCCCC GAGGAGGCCC ACCGCGCCGG CCTCATCGAC 
TGGAACATGT TTGTCAAGCT CAGGAGCCAG GAGTGCGACT GGGAGGAGAT CTCCGTCAAG 
GGCCCCGGAG GCGAGTCCTC CGTGATCCAT GACAGGAAGT CCGGCAGGAA GTTCTCCATT 
GAAGAGGCCC TGCAGAGCGG GCGGCTGACC CCCGCGCAGT ACGACCGCTA CGTCAACAAG 
AGCATGTCCA TCCAGGAGCT GGCCGTCCTG GTGTCAGGGC AGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002697934.3
CDS	225..5492
Translation	MNSLFRKRNKGKYSPTVRTRSVSNKELSELIEQLQKNADQVERNIVDTEAKMQRDLARLQEGQQPEHRDVALQKVSDSEKLLYMLEADAAIAKHMKHPQGDMIAEDIRQLKERVTNLRGKHKQIYNLVVKEVDPQVNWEALVEEKLDELSSQGFGTELPLVGQQVEQHNVFHNEVKAIGPHLAKEQNGELQAKYQKLLAASQARQQHLSSLQDYMQRCTNELYWLDQQAQGRIQYDWSDRNLNYPSRRRQYENFIHRNLEAKEERINKLHSEGDQLLAAEHPGRNSIEAHMEAVHADWKEYLNLLICEESHLKYMEDYHQFHKDMKDAQELLRKVDSDLNQKYSPDFKDRYQIELLLRELDDQEKALDKYQHVVRGLQKRGQQVVPLKYRRETPLKPIPVEALCDFEGDQGLISRGYSYTLQRNNGESWDLTDSAGNKLTAPAVCFMIPPTDPEALALAESLGSQYQSLRQKAAGSRKALQEQHEVLKAESSGDASDLQGRQLLAGLDKVASDLDRQEKAITAILRPPLEQGRAVQDSAERAKDLKNITNELLRIEPEKARSTAEGEAFVRALPDSGSTALLRTRVEDTQRRYERLVQLLEAAQEKLDIANRLEKSLQQGREMLATYETQLAQEDTVPESGQALDSRRQELAAMASELQARKALLGEVQRNLQAAKQCSGSLASRFQEHCPDLERQEAEVHKLCQRFDGLCQQAELRVQSLQSARAEHDAFCSGRDRLLQFLSHIPSYEPQETDGLGQVETKLNNQKNLLDEIARREEEVQKVHTHSQQYQQAVKDYELEAEKLRSLLDLENGRSSHVSKRARLQSPATKVREEEAALAAKFTEVNAINRQRLQNLEFALTLLRQQLEAGASQEALPGSKPGPGAEETWKIQEELDEEMERRQQLEHEVRSAQEEIRALRSQSPQEAVVTREVLRKVPDPALEESFQQAQQTLAEEQHKNRLLQGEVEALRLRLRALEEAARAGGQEYVVKEVLRIEPDRAQADEVLRLRGELEVLRRQKGAREAEALLLQQRVAALAEEKRRAREVVTEKEVVKVHNDPQLEAEFRQLQEDQQREQGLREKQEEELGFLQDKLKRLEKERAMAEGKITVKEVLKVEKDVAAEREVGALQRRYEDEAARARASQREKTELLRKIWALEEENAKVVVQEKVREIVRPDPKAESEVANLRLELVEQERKYRGAEEQLKSYQSELEALRRRGPQVEVKEVTKEVIKYTTDPETEKELQRLREEIVDKTRLIERCDLEIYQLKQEIQSLKEAKPQVQTKEVVQEILQFQEDPQTKEEVAALRARLAEEHKKQVDLERECVSQEEKIQEKEEELARGKEVVVQQEVVRYEEEPGLRAEVSAFTESIDAELRQIDGLRGELRRLQRRRAELEHQLEELERERQARREAELEVQHLKQQLAQLEAQEGEAHEKVTLTQKVVLQQDPQQAREHALLALQLEEERHRRQGLERELETLKQQLEHLEKMEVKEKVVFSESVQVEKGDTEQEIERLRGSLEAESRGKRELDAEVSRLEAKLSELEFCNSKSSKELDFLREENHKLQLERQSLQLEARRLQSEIEMAKAEAQSLRSLPTTTGADPGAQLDSRLRSLERELDDLRRLSRDKDQEIEELQRRLGSVAIKREQRENHLRRSIVVIDPDSGRELSPEEAHRAGLIDWNMFVKLRSQECDWEEISVKGPGGESSVIHDRKSGRKFSIEEALQSGRLTPAQYDRYVNKSMSIQELAVLVSGQK

Target ORF information:

RefSeq Version	XM_002697888.6
Organism	Bos taurus(cattle)
Definition	Bos taurus periplakin (PPL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002697888.6

ORF Insert Sequence:

ATGAACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCCACGGT TCGGACCCGC 
AGCGTCTCTA ACAAGGAGCT GTCGGAGCTC ATCGAGCAGT TGCAGAAGAA TGCCGACCAG 
GTGGAGAGGA ACATCGTGGA CACGGAAGCC AAGATGCAGA GAGACCTGGC CCGGCTGCAG 
GAGGGCCAGC AGCCCGAGCA CCGGGATGTG GCCCTGCAGA AGGTGTCAGA CTCTGAGAAG 
CTGCTCTACA TGCTGGAGGC GGACGCGGCC ATCGCCAAGC ACATGAAGCA CCCCCAGGGG 
GACATGATCG CCGAGGACAT CCGTCAGCTG AAGGAGCGTG TGACCAACCT CCGCGGGAAA 
CACAAGCAGA TCTACAACCT GGTGGTGAAA GAGGTGGACC CACAGGTCAA CTGGGAGGCG 
CTGGTGGAGG AAAAGCTGGA CGAGCTGAGC AGCCAGGGCT TCGGGACGGA ACTCCCGCTG 
GTAGGCCAGC AGGTGGAGCA ACACAACGTC TTTCACAACG AGGTCAAGGC CATCGGGCCC 
CACCTGGCCA AGGAGCAGAA CGGTGAACTC CAGGCCAAGT ACCAGAAACT GCTGGCGGCG 
TCACAGGCGC GGCAGCAGCA CCTGAGCTCA CTGCAGGACT ACATGCAGCG GTGCACGAAC 
GAGCTGTACT GGCTGGACCA GCAGGCCCAG GGCCGCATAC AGTATGACTG GAGTGACCGC 
AACCTCAACT ACCCCAGCCG CCGGCGCCAG TACGAGAATT TCATCCACCG GAACCTGGAG 
GCCAAAGAGG AGAGAATTAA CAAGCTGCAC AGCGAGGGTG ACCAGCTGCT CGCCGCCGAG 
CACCCCGGGA GGAACTCCAT TGAAGCTCAC ATGGAGGCTG TCCACGCAGA TTGGAAGGAA 
TACCTGAACC TGCTCATCTG CGAGGAGAGC CACCTGAAGT ACATGGAAGA CTACCACCAG 
TTCCACAAAG ACATGAAGGA TGCCCAGGAG CTGCTTCGCA AGGTGGACTC AGACCTGAAC 
CAGAAATACA GCCCTGACTT CAAGGACCGA TACCAGATTG AGCTGCTGCT GCGGGAGCTG 
GATGACCAGG AGAAGGCTCT GGACAAGTAC CAGCATGTGG TGCGGGGGCT GCAGAAGCGA 
GGGCAGCAGG TGGTGCCCCT CAAGTACCGC CGGGAGACGC CCCTCAAGCC CATCCCCGTG 
GAGGCGCTCT GTGACTTCGA GGGTGACCAG GGCCTGATCT CTCGGGGCTA CAGCTACACC 
CTGCAGAGGA ACAATGGGGA GAGCTGGGAC CTCACCGACA GTGCGGGGAA CAAGCTGACC 
GCCCCGGCCG TCTGCTTCAT GATCCCGCCC ACTGACCCCG AGGCCCTGGC TCTGGCTGAA 
AGCCTGGGCA GCCAGTACCA GAGCCTGCGG CAGAAGGCAG CTGGGAGCAG AAAGGCGCTC 
CAGGAGCAGC ATGAGGTGCT GAAGGCAGAG AGCTCTGGAG ATGCCTCGGA CCTGCAGGGC 
CGCCAGCTGC TGGCCGGCTT GGACAAGGTG GCCAGCGACC TGGACCGGCA GGAGAAGGCC 
ATCACGGCCA TCCTGCGGCC GCCGCTGGAG CAGGGCCGGG CTGTGCAGGA CAGCGCCGAG 
AGGGCCAAGG ACCTCAAGAA CATCACCAAC GAGCTGCTGC GGATCGAGCC CGAGAAGGCG 
CGCAGCACGG CCGAGGGCGA GGCGTTCGTG CGGGCCCTCC CGGACAGCGG CAGCACCGCC 
CTGCTGAGGA CGCGGGTGGA GGACACCCAG CGCAGATACG AGCGCCTGGT GCAGCTGCTG 
GAAGCCGCCC AGGAGAAGCT CGACATCGCC AACCGCCTGG AGAAGAGCCT GCAGCAGGGC 
CGGGAGATGC TGGCCACGTA TGAGACCCAG CTGGCCCAGG AGGACACGGT GCCCGAGAGT 
GGCCAGGCCC TGGACAGCAG GAGGCAGGAG CTGGCGGCCA TGGCCTCCGA GCTGCAGGCC 
CGGAAGGCCC TCCTCGGCGA GGTGCAGCGG AACCTGCAGG CCGCCAAGCA GTGCTCGGGC 
TCGCTGGCCA GCCGCTTCCA GGAGCACTGC CCGGACCTGG AGCGCCAGGA GGCCGAGGTG 
CACAAGCTGT GCCAGCGCTT CGACGGCCTC TGCCAGCAGG CGGAGCTCAG GGTCCAGAGC 
CTGCAGAGCG CCCGGGCTGA GCACGACGCC TTCTGCAGCG GCCGTGACCG CCTGCTTCAG 
TTCCTGTCCC ACATCCCCAG CTACGAGCCC CAGGAGACGG ACGGCCTCGG CCAAGTGGAG 
ACCAAGCTGA ACAACCAGAA GAACCTACTA GATGAAATAG CAAGAAGGGA AGAGGAAGTG 
CAGAAAGTCC ACACGCACTC CCAGCAGTAC CAGCAGGCTG TCAAGGACTA CGAGTTGGAA 
GCAGAGAAAC TCAGGTCCCT CCTTGACTTG GAGAATGGAA GGAGCAGCCA CGTGAGCAAG 
CGGGCCAGGC TCCAGTCCCC TGCCACCAAA GTGAGGGAAG AGGAAGCAGC TCTTGCTGCC 
AAGTTTACGG AGGTTAATGC CATCAACAGA CAGAGACTGC AGAACCTGGA GTTTGCACTG 
ACCCTCCTGA GACAGCAGCT GGAGGCAGGT GCAAGCCAGG AGGCCCTGCC CGGGAGCAAG 
CCAGGCCCTG GAGCAGAGGA GACGTGGAAG ATTCAGGAGG AGCTGGATGA GGAGATGGAG 
CGGCGGCAGC AGCTGGAGCA CGAGGTCCGG AGCGCCCAGG AGGAGATCCG GGCCCTGCGG 
AGCCAGAGCC CGCAGGAGGC GGTGGTGACC AGGGAGGTGC TCAGGAAGGT GCCGGACCCA 
GCACTGGAGG AGAGCTTCCA GCAGGCGCAG CAGACCCTGG CCGAGGAGCA GCACAAGAAC 
CGGCTGCTGC AGGGGGAGGT GGAGGCGCTG CGGCTGCGGC TGCGTGCCCT GGAGGAGGCG 
GCCCGGGCCG GGGGCCAGGA GTACGTGGTC AAGGAGGTAC TGCGCATTGA GCCGGATCGG 
GCCCAGGCTG ACGAGGTCCT GAGGCTGCGG GGGGAGCTGG AGGTGCTGCG GCGGCAGAAG 
GGCGCCCGCG AGGCGGAGGC GCTTCTCCTG CAGCAGCGCG TGGCCGCCCT GGCCGAGGAG 
AAGCGGCGGG CGCGGGAGGT GGTCACAGAG AAGGAGGTGG TCAAGGTGCA CAATGACCCC 
CAGCTGGAGG CCGAGTTCCG GCAGCTGCAG GAGGACCAGC AGCGGGAGCA GGGGCTCCGG 
GAGAAGCAGG AGGAGGAGCT GGGCTTCCTG CAGGACAAGC TCAAGAGGCT GGAGAAGGAA 
CGGGCCATGG CCGAGGGCAA GATCACAGTC AAGGAGGTGC TCAAGGTGGA GAAGGACGTG 
GCGGCTGAGC GGGAGGTGGG CGCCCTCCAG CGCCGGTATG AGGATGAGGC CGCCAGGGCC 
CGTGCCAGCC AGAGGGAGAA GACCGAGCTG CTCCGGAAGA TCTGGGCCCT GGAGGAGGAG 
AACGCTAAGG TGGTTGTGCA AGAGAAGGTG CGCGAGATTG TCCGGCCTGA CCCCAAGGCT 
GAGAGTGAGG TGGCCAACCT CCGCCTGGAG CTGGTAGAGC AGGAGCGCAA GTACCGGGGG 
GCTGAGGAGC AGCTCAAGAG CTACCAGAGC GAGCTGGAGG CGCTTCGGAG GCGAGGCCCC 
CAGGTAGAGG TCAAGGAGGT GACCAAGGAG GTCATCAAGT ACACGACGGA CCCTGAGACG 
GAGAAGGAGC TCCAGAGGCT CAGGGAGGAG ATCGTGGACA AGACCCGCCT CATCGAGAGG 
TGTGACCTGG AGATCTACCA GCTGAAGCAG GAGATCCAGT CCCTCAAAGA GGCCAAGCCC 
CAGGTGCAGA CCAAGGAGGT GGTCCAGGAG ATCTTGCAGT TCCAGGAAGA CCCCCAGACC 
AAAGAGGAAG TGGCGGCTCT GAGGGCCCGG CTGGCTGAGG AGCACAAGAA GCAGGTAGAC 
CTGGAACGGG AATGCGTGTC ACAGGAGGAG AAGATCCAGG AGAAGGAGGA GGAGCTGGCC 
CGCGGGAAGG AGGTGGTGGT GCAGCAGGAG GTGGTCAGGT ACGAGGAGGA GCCGGGCCTC 
CGGGCGGAAG TGAGCGCCTT CACCGAGAGC ATCGATGCAG AACTGCGGCA GATCGACGGC 
CTCCGCGGCG AGCTGCGGCG GCTGCAGCGC CGGCGAGCCG AGCTGGAGCA CCAGCTGGAG 
GAGCTGGAGC GCGAGCGGCA GGCGCGCCGC GAGGCTGAGC TGGAGGTGCA GCACCTCAAG 
CAACAGCTGG CCCAGCTGGA GGCGCAGGAG GGCGAGGCCC ACGAGAAGGT GACCCTCACA 
CAGAAGGTGG TGCTGCAGCA GGACCCCCAG CAGGCCCGGG AGCATGCCCT CCTGGCCCTG 
CAGCTGGAGG AAGAGCGGCA CCGCCGGCAG GGCCTGGAGC GCGAGCTCGA GACCCTAAAG 
CAGCAGCTGG AGCACCTGGA GAAGATGGAG GTCAAGGAGA AGGTGGTCTT CTCCGAGAGC 
GTCCAGGTGG AGAAAGGCGA CACCGAGCAG GAGATTGAGA GGCTCAGGGG CAGTCTGGAG 
GCTGAGAGCC GAGGCAAGAG GGAGCTGGAC GCCGAGGTGA GCCGGCTGGA GGCCAAGCTG 
TCGGAGCTGG AGTTCTGCAA CTCTAAGTCA TCCAAGGAGC TGGACTTCCT GAGGGAGGAG 
AACCACAAGC TGCAGCTGGA GCGGCAGAGC CTGCAGCTCG AGGCCCGCAG GCTGCAGTCG 
GAGATCGAGA TGGCCAAGGC CGAGGCGCAG AGCCTAAGAA GCCTGCCCAC AACGACTGGA 
GCCGACCCCG GGGCGCAGCT CGACTCACGC CTGCGGTCCC TGGAGCGGGA GCTGGACGAC 
CTCAGGAGGC TCTCCAGGGA CAAAGACCAG GAGATCGAAG AGCTGCAGCG TCGCCTGGGG 
TCCGTGGCCA TCAAGCGGGA GCAGAGGGAG AACCACCTGC GGCGCTCCAT TGTGGTCATC 
GACCCTGACT CGGGCCGTGA GCTGTCCCCC GAGGAGGCCC ACCGCGCCGG CCTCATCGAC 
TGGAACATGT TTGTCAAGCT CAGGAGCCAG GAGTGCGACT GGGAGGAGAT CTCCGTCAAG 
GGCCCCGGAG GCGAGTCCTC CGTGATCCAT GACAGGAAGT CCGGCAGGAA GTTCTCCATT 
GAAGAGGCCC TGCAGAGCGG GCGGCTGACC CCCGCGCAGT ACGACCGCTA CGTCAACAAG 
AGCATGTCCA TCCAGGAGCT GGCCGTCCTG GTGTCAGGGC AGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPL ( XM_005224663.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224720.1 Bos taurus periplakin (PPL), transcript variant X3, mRNA.

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CloneID	OBa32914
Clone ID Related Accession (Same CDS sequence)	XM_005224663.3 , XM_010799955.2 , XM_005224663.4
Accession Version	XM_005224663.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	periplakin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224663.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCCACGGT TCGGACCCGC 
AGCGTCTCTA ACAAGGAGCT GTCGGAGCTC ATCGAGCAGT TGCAGAAGAA TGCCGACCAG 
GTGGAGAGGA ACATCGTGGA CACGGAAGCC AAGATGCAGA GAGACCTGGC CCGGCTGCAG 
GAGGGCCAGC AGCCCGAGCA CCGGGATGTG GCCCTGCAGA AGGTGTCAGA CTCTGAGAAG 
CTGCTCTACA TGCTGGAGGC GGACGCGGCC ATCGCCAAGC ACATGAAGCA CCCCCAGGGG 
GACATGATCG CCGAGGACAT CCGTCAGCTG AAGGAGCGTG TGACCAACCT CCGCGGGAAA 
CACAAGCAGA TCTACAACCT GGTGGTGAAA GAGGTGGACC CACAGGTCAA CTGGGAGGCG 
CTGGTGGAGG AAAAGCTGGA CGAGCTGAGC AGCCAGGGCT TCGGGACGGA ACTCCCGCTG 
GTAGGCCAGC AGGTGGAGCA ACACAACGTC TTTCACAACG AGGTCAAGGC CATCGGGCCC 
CACCTGGCCA AGGAGCAGAA CGGTGAACTC CAGGCCAAGT ACCAGAAACT GCTGGCGGCG 
TCACAGGCGC GGCAGCAGCA CCTGAGCTCA CTGCAGGACT ACATGCAGCG GTGCACGAAC 
GAGCTGTACT GGCTGGACCA GCAGGCCCAG GGCCGCATAC AGTATGACTG GAGTGACCGC 
AACCTCAACT ACCCCAGCCG CCGGCGCCAG TACGAGAATT TCATCCACCG GAACCTGGAG 
GCCAAAGAGG AGAGAATTAA CAAGCTGCAC AGCGAGGGTG ACCAGCTGCT CGCCGCCGAG 
CACCCCGGGA GGAACTCCAT TGAAGCTCAC ATGGAGGCTG TCCACGCAGA TTGGAAGGAA 
TACCTGAACC TGCTCATCTG CGAGGAGAGC CACCTGAAGT ACATGGAAGA CTACCACCAG 
TTCCACAAAG ACATGAAGGA TGCCCAGGAG CTGCTTCGCA AGGTGGACTC AGACCTGAAC 
CAGAAATACA GCCCTGACTT CAAGGACCGA TACCAGATTG AGCTGCTGCT GCGGGAGCTG 
GATGACCAGG AGAAGGCTCT GGACAAGTAC CAGCATGTGG TGCGGGGGCT GCAGAAGCGA 
GGGCAGCAGG TGGTGCCCCT CAAGTACCGC CGGGAGACGC CCCTCAAGCC CATCCCCGTG 
GAGGCGCTCT GTGACTTCGA GGGTGACCAG GGCCTGATCT CTCGGGGCTA CAGCTACACC 
CTGCAGAGGA ACAATGGGGA GAGCTGGGAC CTCACCGACA GTGCGGGGAA CAAGCTGACC 
GCCCCGGCCG TCTGCTTCAT GATCCCGCCC ACTGACCCCG AGGCCCTGGC TCTGGCTGAA 
AGCCTGGGCA GCCAGTACCA GAGCCTGCGG CAGAAGGCAG CTGGGAGCAG AAAGGCGCTC 
CAGGAGCAGC ATGAGGTGCT GAAGGCAGAG AGCTCTGGAG CAGATGCCTC GGACCTGCAG 
GGCCGCCAGC TGCTGGCCGG CTTGGACAAG GTGGCCAGCG ACCTGGACCG GCAGGAGAAG 
GCCATCACGG CCATCCTGCG GCCGCCGCTG GAGCAGGGCC GGGCTGTGCA GGACAGCGCC 
GAGAGGGCCA AGGACCTCAA GAACATCACC AACGAGCTGC TGCGGATCGA GCCCGAGAAG 
GCGCGCAGCA CGGCCGAGGG CGAGGCGTTC GTGCGGGCCC TCCCGGACAG CGGCAGCACC 
GCCCTGCTGA GGACGCGGGT GGAGGACACC CAGCGCAGAT ACGAGCGCCT GGTGCAGCTG 
CTGGAAGCCG CCCAGGAGAA GCTCGACATC GCCAACCGCC TGGAGAAGAG CCTGCAGCAG 
GGCCGGGAGA TGCTGGCCAC GTATGAGACC CAGCTGGCCC AGGAGGACAC GGTGCCCGAG 
AGTGGCCAGG CCCTGGACAG CAGGAGGCAG GAGCTGGCGG CCATGGCCTC CGAGCTGCAG 
GCCCGGAAGG CCCTCCTCGG CGAGGTGCAG CGGAACCTGC AGGCCGCCAA GCAGTGCTCG 
GGCTCGCTGG CCAGCCGCTT CCAGGAGCAC TGCCCGGACC TGGAGCGCCA GGAGGCCGAG 
GTGCACAAGC TGTGCCAGCG CTTCGACGGC CTCTGCCAGC AGGCGGAGCT CAGGGTCCAG 
AGCCTGCAGA GCGCCCGGGC TGAGCACGAC GCCTTCTGCA GCGGCCGTGA CCGCCTGCTT 
CAGTTCCTGT CCCACATCCC CAGCTACGAG CCCCAGGAGA CGGACGGCCT CGGCCAAGTG 
GAGACCAAGC TGAACAACCA GAAGAACCTA CTAGATGAAA TAGCAAGAAG GGAAGAGGAA 
GTGCAGAAAG TCCACACGCA CTCCCAGCAG TACCAGCAGG CTGTCAAGGA CTACGAGTTG 
GAAGCAGAGA AACTCAGGTC CCTCCTTGAC TTGGAGAATG GAAGGAGCAG CCACGTGAGC 
AAGCGGGCCA GGCTCCAGTC CCCTGCCACC AAAGTGAGGG AAGAGGAAGC AGCTCTTGCT 
GCCAAGTTTA CGGAGGTTAA TGCCATCAAC AGACAGAGAC TGCAGAACCT GGAGTTTGCA 
CTGACCCTCC TGAGACAGCT GGAGGCAGGT GCAAGCCAGG AGGCCCTGCC CGGGAGCAAG 
CCAGGCCCTG GAGCAGAGGA GACGTGGAAG ATTCAGGAGG AGCTGGATGA GGAGATGGAG 
CGGCGGCAGC AGCTGGAGCA CGAGGTCCGG AGCGCCCAGG AGGAGATCCG GGCCCTGCGG 
AGCCAGAGCC CGCAGGAGGC GGTGGTGACC AGGGAGGTGC TCAGGAAGGT GCCGGACCCA 
GCACTGGAGG AGAGCTTCCA GCAGGCGCAG CAGACCCTGG CCGAGGAGCA GCACAAGAAC 
CGGCTGCTGC AGGGGGAGGT GGAGGCGCTG CGGCTGCGGC TGCGTGCCCT GGAGGAGGCG 
GCCCGGGCCG GGGGCCAGGA GTACGTGGTC AAGGAGGTAC TGCGCATTGA GCCGGATCGG 
GCCCAGGCTG ACGAGGTCCT GAGGCTGCGG GGGGAGCTGG AGGTGCTGCG GCGGCAGAAG 
GGCGCCCGCG AGGCGGAGGC GCTTCTCCTG CAGCAGCGCG TGGCCGCCCT GGCCGAGGAG 
AAGCGGCGGG CGCGGGAGGT GGTCACAGAG AAGGAGGTGG TCAAGGTGCA CAATGACCCC 
CAGCTGGAGG CCGAGTTCCG GCAGCTGCAG GAGGACCAGC AGCGGGAGCA GGGGCTCCGG 
GAGAAGCAGG AGGAGGAGCT GGGCTTCCTG CAGGACAAGC TCAAGAGGCT GGAGAAGGAA 
CGGGCCATGG CCGAGGGCAA GATCACAGTC AAGGAGGTGC TCAAGGTGGA GAAGGACGTG 
GCGGCTGAGC GGGAGGTGGG CGCCCTCCAG CGCCGGTATG AGGATGAGGC CGCCAGGGCC 
CGTGCCAGCC AGAGGGAGAA GACCGAGCTG CTCCGGAAGA TCTGGGCCCT GGAGGAGGAG 
AACGCTAAGG TGGTTGTGCA AGAGAAGGTG CGCGAGATTG TCCGGCCTGA CCCCAAGGCT 
GAGAGTGAGG TGGCCAACCT CCGCCTGGAG CTGGTAGAGC AGGAGCGCAA GTACCGGGGG 
GCTGAGGAGC AGCTCAAGAG CTACCAGAGC GAGCTGGAGG CGCTTCGGAG GCGAGGCCCC 
CAGGTAGAGG TCAAGGAGGT GACCAAGGAG GTCATCAAGT ACACGACGGA CCCTGAGACG 
GAGAAGGAGC TCCAGAGGCT CAGGGAGGAG ATCGTGGACA AGACCCGCCT CATCGAGAGG 
TGTGACCTGG AGATCTACCA GCTGAAGCAG GAGATCCAGT CCCTCAAAGA GGCCAAGCCC 
CAGGTGCAGA CCAAGGAGGT GGTCCAGGAG ATCTTGCAGT TCCAGGAAGA CCCCCAGACC 
AAAGAGGAAG TGGCGGCTCT GAGGGCCCGG CTGGCTGAGG AGCACAAGAA GCAGGTAGAC 
CTGGAACGGG AATGCGTGTC ACAGGAGGAG AAGATCCAGG AGAAGGAGGA GGAGCTGGCC 
CGCGGGAAGG AGGTGGTGGT GCAGCAGGAG GTGGTCAGGT ACGAGGAGGA GCCGGGCCTC 
CGGGCGGAAG TGAGCGCCTT CACCGAGAGC ATCGATGCAG AACTGCGGCA GATCGACGGC 
CTCCGCGGCG AGCTGCGGCG GCTGCAGCGC CGGCGAGCCG AGCTGGAGCA CCAGCTGGAG 
GAGCTGGAGC GCGAGCGGCA GGCGCGCCGC GAGGCTGAGC TGGAGGTGCA GCACCTCAAG 
CAACAGCTGG CCCAGCTGGA GGCGCAGGAG GGCGAGGCCC ACGAGAAGGT GACCCTCACA 
CAGAAGGTGG TGCTGCAGCA GGACCCCCAG CAGGCCCGGG AGCATGCCCT CCTGGCCCTG 
CAGCTGGAGG AAGAGCGGCA CCGCCGGCAG GGCCTGGAGC GCGAGCTCGA GACCCTAAAG 
CAGCAGCTGG AGCACCTGGA GAAGATGGAG GTCAAGGAGA AGGTGGTCTT CTCCGAGAGC 
GTCCAGGTGG AGAAAGGCGA CACCGAGCAG GAGATTGAGA GGCTCAGGGG CAGTCTGGAG 
GCTGAGAGCC GAGGCAAGAG GGAGCTGGAC GCCGAGGTGA GCCGGCTGGA GGCCAAGCTG 
TCGGAGCTGG AGTTCTGCAA CTCTAAGTCA TCCAAGGAGC TGGACTTCCT GAGGGAGGAG 
AACCACAAGC TGCAGCTGGA GCGGCAGAGC CTGCAGCTCG AGGCCCGCAG GCTGCAGTCG 
GAGATCGAGA TGGCCAAGGC CGAGGCGCAG AGCCTAAGAA GCCTGCCCAC AACGACTGGA 
GCCGACCCCG GGGCGCAGCT CGACTCACGC CTGCGGTCCC TGGAGCGGGA GCTGGACGAC 
CTCAGGAGGC TCTCCAGGGA CAAAGACCAG GAGATCGAAG AGCTGCAGCG TCGCCTGGGG 
TCCGTGGCCA TCAAGCGGGA GCAGAGGGAG AACCACCTGC GGCGCTCCAT TGTGGTCATC 
GACCCTGACT CGGGCCGTGA GCTGTCCCCC GAGGAGGCCC ACCGCGCCGG CCTCATCGAC 
TGGAACATGT TTGTCAAGCT CAGGAGCCAG GAGTGCGACT GGGAGGAGAT CTCCGTCAAG 
GGCCCCGGAG GCGAGTCCTC CGTGATCCAT GACAGGAAGT CCGGCAGGAA GTTCTCCATT 
GAAGAGGCCC TGCAGAGCGG GCGGCTGACC CCCGCGCAGT ACGACCGCTA CGTCAACAAG 
AGCATGTCCA TCCAGGAGCT GGCCGTCCTG GTGTCAGGGC AGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224720.1
CDS	207..5474
Translation	MNSLFRKRNKGKYSPTVRTRSVSNKELSELIEQLQKNADQVERNIVDTEAKMQRDLARLQEGQQPEHRDVALQKVSDSEKLLYMLEADAAIAKHMKHPQGDMIAEDIRQLKERVTNLRGKHKQIYNLVVKEVDPQVNWEALVEEKLDELSSQGFGTELPLVGQQVEQHNVFHNEVKAIGPHLAKEQNGELQAKYQKLLAASQARQQHLSSLQDYMQRCTNELYWLDQQAQGRIQYDWSDRNLNYPSRRRQYENFIHRNLEAKEERINKLHSEGDQLLAAEHPGRNSIEAHMEAVHADWKEYLNLLICEESHLKYMEDYHQFHKDMKDAQELLRKVDSDLNQKYSPDFKDRYQIELLLRELDDQEKALDKYQHVVRGLQKRGQQVVPLKYRRETPLKPIPVEALCDFEGDQGLISRGYSYTLQRNNGESWDLTDSAGNKLTAPAVCFMIPPTDPEALALAESLGSQYQSLRQKAAGSRKALQEQHEVLKAESSGADASDLQGRQLLAGLDKVASDLDRQEKAITAILRPPLEQGRAVQDSAERAKDLKNITNELLRIEPEKARSTAEGEAFVRALPDSGSTALLRTRVEDTQRRYERLVQLLEAAQEKLDIANRLEKSLQQGREMLATYETQLAQEDTVPESGQALDSRRQELAAMASELQARKALLGEVQRNLQAAKQCSGSLASRFQEHCPDLERQEAEVHKLCQRFDGLCQQAELRVQSLQSARAEHDAFCSGRDRLLQFLSHIPSYEPQETDGLGQVETKLNNQKNLLDEIARREEEVQKVHTHSQQYQQAVKDYELEAEKLRSLLDLENGRSSHVSKRARLQSPATKVREEEAALAAKFTEVNAINRQRLQNLEFALTLLRQLEAGASQEALPGSKPGPGAEETWKIQEELDEEMERRQQLEHEVRSAQEEIRALRSQSPQEAVVTREVLRKVPDPALEESFQQAQQTLAEEQHKNRLLQGEVEALRLRLRALEEAARAGGQEYVVKEVLRIEPDRAQADEVLRLRGELEVLRRQKGAREAEALLLQQRVAALAEEKRRAREVVTEKEVVKVHNDPQLEAEFRQLQEDQQREQGLREKQEEELGFLQDKLKRLEKERAMAEGKITVKEVLKVEKDVAAEREVGALQRRYEDEAARARASQREKTELLRKIWALEEENAKVVVQEKVREIVRPDPKAESEVANLRLELVEQERKYRGAEEQLKSYQSELEALRRRGPQVEVKEVTKEVIKYTTDPETEKELQRLREEIVDKTRLIERCDLEIYQLKQEIQSLKEAKPQVQTKEVVQEILQFQEDPQTKEEVAALRARLAEEHKKQVDLERECVSQEEKIQEKEEELARGKEVVVQQEVVRYEEEPGLRAEVSAFTESIDAELRQIDGLRGELRRLQRRRAELEHQLEELERERQARREAELEVQHLKQQLAQLEAQEGEAHEKVTLTQKVVLQQDPQQAREHALLALQLEEERHRRQGLERELETLKQQLEHLEKMEVKEKVVFSESVQVEKGDTEQEIERLRGSLEAESRGKRELDAEVSRLEAKLSELEFCNSKSSKELDFLREENHKLQLERQSLQLEARRLQSEIEMAKAEAQSLRSLPTTTGADPGAQLDSRLRSLERELDDLRRLSRDKDQEIEELQRRLGSVAIKREQRENHLRRSIVVIDPDSGRELSPEEAHRAGLIDWNMFVKLRSQECDWEEISVKGPGGESSVIHDRKSGRKFSIEEALQSGRLTPAQYDRYVNKSMSIQELAVLVSGQK

Target ORF information:

RefSeq Version	XM_005224663.3
Organism	Bos taurus(cattle)
Definition	Bos taurus periplakin (PPL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224663.3

ORF Insert Sequence:

ATGAACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCCACGGT TCGGACCCGC 
AGCGTCTCTA ACAAGGAGCT GTCGGAGCTC ATCGAGCAGT TGCAGAAGAA TGCCGACCAG 
GTGGAGAGGA ACATCGTGGA CACGGAAGCC AAGATGCAGA GAGACCTGGC CCGGCTGCAG 
GAGGGCCAGC AGCCCGAGCA CCGGGATGTG GCCCTGCAGA AGGTGTCAGA CTCTGAGAAG 
CTGCTCTACA TGCTGGAGGC GGACGCGGCC ATCGCCAAGC ACATGAAGCA CCCCCAGGGG 
GACATGATCG CCGAGGACAT CCGTCAGCTG AAGGAGCGTG TGACCAACCT CCGCGGGAAA 
CACAAGCAGA TCTACAACCT GGTGGTGAAA GAGGTGGACC CACAGGTCAA CTGGGAGGCG 
CTGGTGGAGG AAAAGCTGGA CGAGCTGAGC AGCCAGGGCT TCGGGACGGA ACTCCCGCTG 
GTAGGCCAGC AGGTGGAGCA ACACAACGTC TTTCACAACG AGGTCAAGGC CATCGGGCCC 
CACCTGGCCA AGGAGCAGAA CGGTGAACTC CAGGCCAAGT ACCAGAAACT GCTGGCGGCG 
TCACAGGCGC GGCAGCAGCA CCTGAGCTCA CTGCAGGACT ACATGCAGCG GTGCACGAAC 
GAGCTGTACT GGCTGGACCA GCAGGCCCAG GGCCGCATAC AGTATGACTG GAGTGACCGC 
AACCTCAACT ACCCCAGCCG CCGGCGCCAG TACGAGAATT TCATCCACCG GAACCTGGAG 
GCCAAAGAGG AGAGAATTAA CAAGCTGCAC AGCGAGGGTG ACCAGCTGCT CGCCGCCGAG 
CACCCCGGGA GGAACTCCAT TGAAGCTCAC ATGGAGGCTG TCCACGCAGA TTGGAAGGAA 
TACCTGAACC TGCTCATCTG CGAGGAGAGC CACCTGAAGT ACATGGAAGA CTACCACCAG 
TTCCACAAAG ACATGAAGGA TGCCCAGGAG CTGCTTCGCA AGGTGGACTC AGACCTGAAC 
CAGAAATACA GCCCTGACTT CAAGGACCGA TACCAGATTG AGCTGCTGCT GCGGGAGCTG 
GATGACCAGG AGAAGGCTCT GGACAAGTAC CAGCATGTGG TGCGGGGGCT GCAGAAGCGA 
GGGCAGCAGG TGGTGCCCCT CAAGTACCGC CGGGAGACGC CCCTCAAGCC CATCCCCGTG 
GAGGCGCTCT GTGACTTCGA GGGTGACCAG GGCCTGATCT CTCGGGGCTA CAGCTACACC 
CTGCAGAGGA ACAATGGGGA GAGCTGGGAC CTCACCGACA GTGCGGGGAA CAAGCTGACC 
GCCCCGGCCG TCTGCTTCAT GATCCCGCCC ACTGACCCCG AGGCCCTGGC TCTGGCTGAA 
AGCCTGGGCA GCCAGTACCA GAGCCTGCGG CAGAAGGCAG CTGGGAGCAG AAAGGCGCTC 
CAGGAGCAGC ATGAGGTGCT GAAGGCAGAG AGCTCTGGAG CAGATGCCTC GGACCTGCAG 
GGCCGCCAGC TGCTGGCCGG CTTGGACAAG GTGGCCAGCG ACCTGGACCG GCAGGAGAAG 
GCCATCACGG CCATCCTGCG GCCGCCGCTG GAGCAGGGCC GGGCTGTGCA GGACAGCGCC 
GAGAGGGCCA AGGACCTCAA GAACATCACC AACGAGCTGC TGCGGATCGA GCCCGAGAAG 
GCGCGCAGCA CGGCCGAGGG CGAGGCGTTC GTGCGGGCCC TCCCGGACAG CGGCAGCACC 
GCCCTGCTGA GGACGCGGGT GGAGGACACC CAGCGCAGAT ACGAGCGCCT GGTGCAGCTG 
CTGGAAGCCG CCCAGGAGAA GCTCGACATC GCCAACCGCC TGGAGAAGAG CCTGCAGCAG 
GGCCGGGAGA TGCTGGCCAC GTATGAGACC CAGCTGGCCC AGGAGGACAC GGTGCCCGAG 
AGTGGCCAGG CCCTGGACAG CAGGAGGCAG GAGCTGGCGG CCATGGCCTC CGAGCTGCAG 
GCCCGGAAGG CCCTCCTCGG CGAGGTGCAG CGGAACCTGC AGGCCGCCAA GCAGTGCTCG 
GGCTCGCTGG CCAGCCGCTT CCAGGAGCAC TGCCCGGACC TGGAGCGCCA GGAGGCCGAG 
GTGCACAAGC TGTGCCAGCG CTTCGACGGC CTCTGCCAGC AGGCGGAGCT CAGGGTCCAG 
AGCCTGCAGA GCGCCCGGGC TGAGCACGAC GCCTTCTGCA GCGGCCGTGA CCGCCTGCTT 
CAGTTCCTGT CCCACATCCC CAGCTACGAG CCCCAGGAGA CGGACGGCCT CGGCCAAGTG 
GAGACCAAGC TGAACAACCA GAAGAACCTA CTAGATGAAA TAGCAAGAAG GGAAGAGGAA 
GTGCAGAAAG TCCACACGCA CTCCCAGCAG TACCAGCAGG CTGTCAAGGA CTACGAGTTG 
GAAGCAGAGA AACTCAGGTC CCTCCTTGAC TTGGAGAATG GAAGGAGCAG CCACGTGAGC 
AAGCGGGCCA GGCTCCAGTC CCCTGCCACC AAAGTGAGGG AAGAGGAAGC AGCTCTTGCT 
GCCAAGTTTA CGGAGGTTAA TGCCATCAAC AGACAGAGAC TGCAGAACCT GGAGTTTGCA 
CTGACCCTCC TGAGACAGCT GGAGGCAGGT GCAAGCCAGG AGGCCCTGCC CGGGAGCAAG 
CCAGGCCCTG GAGCAGAGGA GACGTGGAAG ATTCAGGAGG AGCTGGATGA GGAGATGGAG 
CGGCGGCAGC AGCTGGAGCA CGAGGTCCGG AGCGCCCAGG AGGAGATCCG GGCCCTGCGG 
AGCCAGAGCC CGCAGGAGGC GGTGGTGACC AGGGAGGTGC TCAGGAAGGT GCCGGACCCA 
GCACTGGAGG AGAGCTTCCA GCAGGCGCAG CAGACCCTGG CCGAGGAGCA GCACAAGAAC 
CGGCTGCTGC AGGGGGAGGT GGAGGCGCTG CGGCTGCGGC TGCGTGCCCT GGAGGAGGCG 
GCCCGGGCCG GGGGCCAGGA GTACGTGGTC AAGGAGGTAC TGCGCATTGA GCCGGATCGG 
GCCCAGGCTG ACGAGGTCCT GAGGCTGCGG GGGGAGCTGG AGGTGCTGCG GCGGCAGAAG 
GGCGCCCGCG AGGCGGAGGC GCTTCTCCTG CAGCAGCGCG TGGCCGCCCT GGCCGAGGAG 
AAGCGGCGGG CGCGGGAGGT GGTCACAGAG AAGGAGGTGG TCAAGGTGCA CAATGACCCC 
CAGCTGGAGG CCGAGTTCCG GCAGCTGCAG GAGGACCAGC AGCGGGAGCA GGGGCTCCGG 
GAGAAGCAGG AGGAGGAGCT GGGCTTCCTG CAGGACAAGC TCAAGAGGCT GGAGAAGGAA 
CGGGCCATGG CCGAGGGCAA GATCACAGTC AAGGAGGTGC TCAAGGTGGA GAAGGACGTG 
GCGGCTGAGC GGGAGGTGGG CGCCCTCCAG CGCCGGTATG AGGATGAGGC CGCCAGGGCC 
CGTGCCAGCC AGAGGGAGAA GACCGAGCTG CTCCGGAAGA TCTGGGCCCT GGAGGAGGAG 
AACGCTAAGG TGGTTGTGCA AGAGAAGGTG CGCGAGATTG TCCGGCCTGA CCCCAAGGCT 
GAGAGTGAGG TGGCCAACCT CCGCCTGGAG CTGGTAGAGC AGGAGCGCAA GTACCGGGGG 
GCTGAGGAGC AGCTCAAGAG CTACCAGAGC GAGCTGGAGG CGCTTCGGAG GCGAGGCCCC 
CAGGTAGAGG TCAAGGAGGT GACCAAGGAG GTCATCAAGT ACACGACGGA CCCTGAGACG 
GAGAAGGAGC TCCAGAGGCT CAGGGAGGAG ATCGTGGACA AGACCCGCCT CATCGAGAGG 
TGTGACCTGG AGATCTACCA GCTGAAGCAG GAGATCCAGT CCCTCAAAGA GGCCAAGCCC 
CAGGTGCAGA CCAAGGAGGT GGTCCAGGAG ATCTTGCAGT TCCAGGAAGA CCCCCAGACC 
AAAGAGGAAG TGGCGGCTCT GAGGGCCCGG CTGGCTGAGG AGCACAAGAA GCAGGTAGAC 
CTGGAACGGG AATGCGTGTC ACAGGAGGAG AAGATCCAGG AGAAGGAGGA GGAGCTGGCC 
CGCGGGAAGG AGGTGGTGGT GCAGCAGGAG GTGGTCAGGT ACGAGGAGGA GCCGGGCCTC 
CGGGCGGAAG TGAGCGCCTT CACCGAGAGC ATCGATGCAG AACTGCGGCA GATCGACGGC 
CTCCGCGGCG AGCTGCGGCG GCTGCAGCGC CGGCGAGCCG AGCTGGAGCA CCAGCTGGAG 
GAGCTGGAGC GCGAGCGGCA GGCGCGCCGC GAGGCTGAGC TGGAGGTGCA GCACCTCAAG 
CAACAGCTGG CCCAGCTGGA GGCGCAGGAG GGCGAGGCCC ACGAGAAGGT GACCCTCACA 
CAGAAGGTGG TGCTGCAGCA GGACCCCCAG CAGGCCCGGG AGCATGCCCT CCTGGCCCTG 
CAGCTGGAGG AAGAGCGGCA CCGCCGGCAG GGCCTGGAGC GCGAGCTCGA GACCCTAAAG 
CAGCAGCTGG AGCACCTGGA GAAGATGGAG GTCAAGGAGA AGGTGGTCTT CTCCGAGAGC 
GTCCAGGTGG AGAAAGGCGA CACCGAGCAG GAGATTGAGA GGCTCAGGGG CAGTCTGGAG 
GCTGAGAGCC GAGGCAAGAG GGAGCTGGAC GCCGAGGTGA GCCGGCTGGA GGCCAAGCTG 
TCGGAGCTGG AGTTCTGCAA CTCTAAGTCA TCCAAGGAGC TGGACTTCCT GAGGGAGGAG 
AACCACAAGC TGCAGCTGGA GCGGCAGAGC CTGCAGCTCG AGGCCCGCAG GCTGCAGTCG 
GAGATCGAGA TGGCCAAGGC CGAGGCGCAG AGCCTAAGAA GCCTGCCCAC AACGACTGGA 
GCCGACCCCG GGGCGCAGCT CGACTCACGC CTGCGGTCCC TGGAGCGGGA GCTGGACGAC 
CTCAGGAGGC TCTCCAGGGA CAAAGACCAG GAGATCGAAG AGCTGCAGCG TCGCCTGGGG 
TCCGTGGCCA TCAAGCGGGA GCAGAGGGAG AACCACCTGC GGCGCTCCAT TGTGGTCATC 
GACCCTGACT CGGGCCGTGA GCTGTCCCCC GAGGAGGCCC ACCGCGCCGG CCTCATCGAC 
TGGAACATGT TTGTCAAGCT CAGGAGCCAG GAGTGCGACT GGGAGGAGAT CTCCGTCAAG 
GGCCCCGGAG GCGAGTCCTC CGTGATCCAT GACAGGAAGT CCGGCAGGAA GTTCTCCATT 
GAAGAGGCCC TGCAGAGCGG GCGGCTGACC CCCGCGCAGT ACGACCGCTA CGTCAACAAG 
AGCATGTCCA TCCAGGAGCT GGCCGTCCTG GTGTCAGGGC AGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPL ( XM_010799955.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010798257.2 Bos taurus periplakin (PPL), transcript variant X3, mRNA.

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CloneID	OBa32914
Clone ID Related Accession (Same CDS sequence)	XM_005224663.3 , XM_010799955.2 , XM_005224663.4
Accession Version	XM_010799955.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	periplakin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646645.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010799955.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCCACGGT TCGGACCCGC 
AGCGTCTCTA ACAAGGAGCT GTCGGAGCTC ATCGAGCAGT TGCAGAAGAA TGCCGACCAG 
GTGGAGAGGA ACATCGTGGA CACGGAAGCC AAGATGCAGA GAGACCTGGC CCGGCTGCAG 
GAGGGCCAGC AGCCCGAGCA CCGGGATGTG GCCCTGCAGA AGGTGTCAGA CTCTGAGAAG 
CTGCTCTACA TGCTGGAGGC GGACGCGGCC ATCGCCAAGC ACATGAAGCA CCCCCAGGGG 
GACATGATCG CCGAGGACAT CCGTCAGCTG AAGGAGCGTG TGACCAACCT CCGCGGGAAA 
CACAAGCAGA TCTACAACCT GGTGGTGAAA GAGGTGGACC CACAGGTCAA CTGGGAGGCG 
CTGGTGGAGG AAAAGCTGGA CGAGCTGAGC AGCCAGGGCT TCGGGACGGA ACTCCCGCTG 
GTAGGCCAGC AGGTGGAGCA ACACAACGTC TTTCACAACG AGGTCAAGGC CATCGGGCCC 
CACCTGGCCA AGGAGCAGAA CGGTGAACTC CAGGCCAAGT ACCAGAAACT GCTGGCGGCG 
TCACAGGCGC GGCAGCAGCA CCTGAGCTCA CTGCAGGACT ACATGCAGCG GTGCACGAAC 
GAGCTGTACT GGCTGGACCA GCAGGCCCAG GGCCGCATAC AGTATGACTG GAGTGACCGC 
AACCTCAACT ACCCCAGCCG CCGGCGCCAG TACGAGAATT TCATCCACCG GAACCTGGAG 
GCCAAAGAGG AGAGAATTAA CAAGCTGCAC AGCGAGGGTG ACCAGCTGCT CGCCGCCGAG 
CACCCCGGGA GGAACTCCAT TGAAGCTCAC ATGGAGGCTG TCCACGCAGA TTGGAAGGAA 
TACCTGAACC TGCTCATCTG CGAGGAGAGC CACCTGAAGT ACATGGAAGA CTACCACCAG 
TTCCACAAAG ACATGAAGGA TGCCCAGGAG CTGCTTCGCA AGGTGGACTC AGACCTGAAC 
CAGAAATACA GCCCTGACTT CAAGGACCGA TACCAGATTG AGCTGCTGCT GCGGGAGCTG 
GATGACCAGG AGAAGGCTCT GGACAAGTAC CAGCATGTGG TGCGGGGGCT GCAGAAGCGA 
GGGCAGCAGG TGGTGCCCCT CAAGTACCGC CGGGAGACGC CCCTCAAGCC CATCCCCGTG 
GAGGCGCTCT GTGACTTCGA GGGTGACCAG GGCCTGATCT CTCGGGGCTA CAGCTACACC 
CTGCAGAGGA ACAATGGGGA GAGCTGGGAC CTCACCGACA GTGCGGGGAA CAAGCTGACC 
GCCCCGGCCG TCTGCTTCAT GATCCCGCCC ACTGACCCCG AGGCCCTGGC TCTGGCTGAA 
AGCCTGGGCA GCCAGTACCA GAGCCTGCGG CAGAAGGCAG CTGGGAGCAG AAAGGCGCTC 
CAGGAGCAGC ATGAGGTGCT GAAGGCAGAG AGCTCTGGAG CAGATGCCTC GGACCTGCAG 
GGCCGCCAGC TGCTGGCCGG CTTGGACAAG GTGGCCAGCG ACCTGGACCG GCAGGAGAAG 
GCCATCACGG CCATCCTGCG GCCGCCGCTG GAGCAGGGCC GGGCTGTGCA GGACAGCGCC 
GAGAGGGCCA AGGACCTCAA GAACATCACC AACGAGCTGC TGCGGATCGA GCCCGAGAAG 
GCGCGCAGCA CGGCCGAGGG CGAGGCGTTC GTGCGGGCCC TCCCGGACAG CGGCAGCACC 
GCCCTGCTGA GGACGCGGGT GGAGGACACC CAGCGCAGAT ACGAGCGCCT GGTGCAGCTG 
CTGGAAGCCG CCCAGGAGAA GCTCGACATC GCCAACCGCC TGGAGAAGAG CCTGCAGCAG 
GGCCGGGAGA TGCTGGCCAC GTATGAGACC CAGCTGGCCC AGGAGGACAC GGTGCCCGAG 
AGTGGCCAGG CCCTGGACAG CAGGAGGCAG GAGCTGGCGG CCATGGCCTC CGAGCTGCAG 
GCCCGGAAGG CCCTCCTCGG CGAGGTGCAG CGGAACCTGC AGGCCGCCAA GCAGTGCTCG 
GGCTCGCTGG CCAGCCGCTT CCAGGAGCAC TGCCCGGACC TGGAGCGCCA GGAGGCCGAG 
GTGCACAAGC TGTGCCAGCG CTTCGACGGC CTCTGCCAGC AGGCGGAGCT CAGGGTCCAG 
AGCCTGCAGA GCGCCCGGGC TGAGCACGAC GCCTTCTGCA GCGGCCGTGA CCGCCTGCTT 
CAGTTCCTGT CCCACATCCC CAGCTACGAG CCCCAGGAGA CGGACGGCCT CGGCCAAGTG 
GAGACCAAGC TGAACAACCA GAAGAACCTA CTAGATGAAA TAGCAAGAAG GGAAGAGGAA 
GTGCAGAAAG TCCACACGCA CTCCCAGCAG TACCAGCAGG CTGTCAAGGA CTACGAGTTG 
GAAGCAGAGA AACTCAGGTC CCTCCTTGAC TTGGAGAATG GAAGGAGCAG CCACGTGAGC 
AAGCGGGCCA GGCTCCAGTC CCCTGCCACC AAAGTGAGGG AAGAGGAAGC AGCTCTTGCT 
GCCAAGTTTA CGGAGGTTAA TGCCATCAAC AGACAGAGAC TGCAGAACCT GGAGTTTGCA 
CTGACCCTCC TGAGACAGCT GGAGGCAGGT GCAAGCCAGG AGGCCCTGCC CGGGAGCAAG 
CCAGGCCCTG GAGCAGAGGA GACGTGGAAG ATTCAGGAGG AGCTGGATGA GGAGATGGAG 
CGGCGGCAGC AGCTGGAGCA CGAGGTCCGG AGCGCCCAGG AGGAGATCCG GGCCCTGCGG 
AGCCAGAGCC CGCAGGAGGC GGTGGTGACC AGGGAGGTGC TCAGGAAGGT GCCGGACCCA 
GCACTGGAGG AGAGCTTCCA GCAGGCGCAG CAGACCCTGG CCGAGGAGCA GCACAAGAAC 
CGGCTGCTGC AGGGGGAGGT GGAGGCGCTG CGGCTGCGGC TGCGTGCCCT GGAGGAGGCG 
GCCCGGGCCG GGGGCCAGGA GTACGTGGTC AAGGAGGTAC TGCGCATTGA GCCGGATCGG 
GCCCAGGCTG ACGAGGTCCT GAGGCTGCGG GGGGAGCTGG AGGTGCTGCG GCGGCAGAAG 
GGCGCCCGCG AGGCGGAGGC GCTTCTCCTG CAGCAGCGCG TGGCCGCCCT GGCCGAGGAG 
AAGCGGCGGG CGCGGGAGGT GGTCACAGAG AAGGAGGTGG TCAAGGTGCA CAATGACCCC 
CAGCTGGAGG CCGAGTTCCG GCAGCTGCAG GAGGACCAGC AGCGGGAGCA GGGGCTCCGG 
GAGAAGCAGG AGGAGGAGCT GGGCTTCCTG CAGGACAAGC TCAAGAGGCT GGAGAAGGAA 
CGGGCCATGG CCGAGGGCAA GATCACAGTC AAGGAGGTGC TCAAGGTGGA GAAGGACGTG 
GCGGCTGAGC GGGAGGTGGG CGCCCTCCAG CGCCGGTATG AGGATGAGGC CGCCAGGGCC 
CGTGCCAGCC AGAGGGAGAA GACCGAGCTG CTCCGGAAGA TCTGGGCCCT GGAGGAGGAG 
AACGCTAAGG TGGTTGTGCA AGAGAAGGTG CGCGAGATTG TCCGGCCTGA CCCCAAGGCT 
GAGAGTGAGG TGGCCAACCT CCGCCTGGAG CTGGTAGAGC AGGAGCGCAA GTACCGGGGG 
GCTGAGGAGC AGCTCAAGAG CTACCAGAGC GAGCTGGAGG CGCTTCGGAG GCGAGGCCCC 
CAGGTAGAGG TCAAGGAGGT GACCAAGGAG GTCATCAAGT ACACGACGGA CCCTGAGACG 
GAGAAGGAGC TCCAGAGGCT CAGGGAGGAG ATCGTGGACA AGACCCGCCT CATCGAGAGG 
TGTGACCTGG AGATCTACCA GCTGAAGCAG GAGATCCAGT CCCTCAAAGA GGCCAAGCCC 
CAGGTGCAGA CCAAGGAGGT GGTCCAGGAG ATCTTGCAGT TCCAGGAAGA CCCCCAGACC 
AAAGAGGAAG TGGCGGCTCT GAGGGCCCGG CTGGCTGAGG AGCACAAGAA GCAGGTAGAC 
CTGGAACGGG AATGCGTGTC ACAGGAGGAG AAGATCCAGG AGAAGGAGGA GGAGCTGGCC 
CGCGGGAAGG AGGTGGTGGT GCAGCAGGAG GTGGTCAGGT ACGAGGAGGA GCCGGGCCTC 
CGGGCGGAAG TGAGCGCCTT CACCGAGAGC ATCGATGCAG AACTGCGGCA GATCGACGGC 
CTCCGCGGCG AGCTGCGGCG GCTGCAGCGC CGGCGAGCCG AGCTGGAGCA CCAGCTGGAG 
GAGCTGGAGC GCGAGCGGCA GGCGCGCCGC GAGGCTGAGC TGGAGGTGCA GCACCTCAAG 
CAACAGCTGG CCCAGCTGGA GGCGCAGGAG GGCGAGGCCC ACGAGAAGGT GACCCTCACA 
CAGAAGGTGG TGCTGCAGCA GGACCCCCAG CAGGCCCGGG AGCATGCCCT CCTGGCCCTG 
CAGCTGGAGG AAGAGCGGCA CCGCCGGCAG GGCCTGGAGC GCGAGCTCGA GACCCTAAAG 
CAGCAGCTGG AGCACCTGGA GAAGATGGAG GTCAAGGAGA AGGTGGTCTT CTCCGAGAGC 
GTCCAGGTGG AGAAAGGCGA CACCGAGCAG GAGATTGAGA GGCTCAGGGG CAGTCTGGAG 
GCTGAGAGCC GAGGCAAGAG GGAGCTGGAC GCCGAGGTGA GCCGGCTGGA GGCCAAGCTG 
TCGGAGCTGG AGTTCTGCAA CTCTAAGTCA TCCAAGGAGC TGGACTTCCT GAGGGAGGAG 
AACCACAAGC TGCAGCTGGA GCGGCAGAGC CTGCAGCTCG AGGCCCGCAG GCTGCAGTCG 
GAGATCGAGA TGGCCAAGGC CGAGGCGCAG AGCCTAAGAA GCCTGCCCAC AACGACTGGA 
GCCGACCCCG GGGCGCAGCT CGACTCACGC CTGCGGTCCC TGGAGCGGGA GCTGGACGAC 
CTCAGGAGGC TCTCCAGGGA CAAAGACCAG GAGATCGAAG AGCTGCAGCG TCGCCTGGGG 
TCCGTGGCCA TCAAGCGGGA GCAGAGGGAG AACCACCTGC GGCGCTCCAT TGTGGTCATC 
GACCCTGACT CGGGCCGTGA GCTGTCCCCC GAGGAGGCCC ACCGCGCCGG CCTCATCGAC 
TGGAACATGT TTGTCAAGCT CAGGAGCCAG GAGTGCGACT GGGAGGAGAT CTCCGTCAAG 
GGCCCCGGAG GCGAGTCCTC CGTGATCCAT GACAGGAAGT CCGGCAGGAA GTTCTCCATT 
GAAGAGGCCC TGCAGAGCGG GCGGCTGACC CCCGCGCAGT ACGACCGCTA CGTCAACAAG 
AGCATGTCCA TCCAGGAGCT GGCCGTCCTG GTGTCAGGGC AGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010798257.2
CDS	207..5474
Translation	MNSLFRKRNKGKYSPTVRTRSVSNKELSELIEQLQKNADQVERNIVDTEAKMQRDLARLQEGQQPEHRDVALQKVSDSEKLLYMLEADAAIAKHMKHPQGDMIAEDIRQLKERVTNLRGKHKQIYNLVVKEVDPQVNWEALVEEKLDELSSQGFGTELPLVGQQVEQHNVFHNEVKAIGPHLAKEQNGELQAKYQKLLAASQARQQHLSSLQDYMQRCTNELYWLDQQAQGRIQYDWSDRNLNYPSRRRQYENFIHRNLEAKEERINKLHSEGDQLLAAEHPGRNSIEAHMEAVHADWKEYLNLLICEESHLKYMEDYHQFHKDMKDAQELLRKVDSDLNQKYSPDFKDRYQIELLLRELDDQEKALDKYQHVVRGLQKRGQQVVPLKYRRETPLKPIPVEALCDFEGDQGLISRGYSYTLQRNNGESWDLTDSAGNKLTAPAVCFMIPPTDPEALALAESLGSQYQSLRQKAAGSRKALQEQHEVLKAESSGADASDLQGRQLLAGLDKVASDLDRQEKAITAILRPPLEQGRAVQDSAERAKDLKNITNELLRIEPEKARSTAEGEAFVRALPDSGSTALLRTRVEDTQRRYERLVQLLEAAQEKLDIANRLEKSLQQGREMLATYETQLAQEDTVPESGQALDSRRQELAAMASELQARKALLGEVQRNLQAAKQCSGSLASRFQEHCPDLERQEAEVHKLCQRFDGLCQQAELRVQSLQSARAEHDAFCSGRDRLLQFLSHIPSYEPQETDGLGQVETKLNNQKNLLDEIARREEEVQKVHTHSQQYQQAVKDYELEAEKLRSLLDLENGRSSHVSKRARLQSPATKVREEEAALAAKFTEVNAINRQRLQNLEFALTLLRQLEAGASQEALPGSKPGPGAEETWKIQEELDEEMERRQQLEHEVRSAQEEIRALRSQSPQEAVVTREVLRKVPDPALEESFQQAQQTLAEEQHKNRLLQGEVEALRLRLRALEEAARAGGQEYVVKEVLRIEPDRAQADEVLRLRGELEVLRRQKGAREAEALLLQQRVAALAEEKRRAREVVTEKEVVKVHNDPQLEAEFRQLQEDQQREQGLREKQEEELGFLQDKLKRLEKERAMAEGKITVKEVLKVEKDVAAEREVGALQRRYEDEAARARASQREKTELLRKIWALEEENAKVVVQEKVREIVRPDPKAESEVANLRLELVEQERKYRGAEEQLKSYQSELEALRRRGPQVEVKEVTKEVIKYTTDPETEKELQRLREEIVDKTRLIERCDLEIYQLKQEIQSLKEAKPQVQTKEVVQEILQFQEDPQTKEEVAALRARLAEEHKKQVDLERECVSQEEKIQEKEEELARGKEVVVQQEVVRYEEEPGLRAEVSAFTESIDAELRQIDGLRGELRRLQRRRAELEHQLEELERERQARREAELEVQHLKQQLAQLEAQEGEAHEKVTLTQKVVLQQDPQQAREHALLALQLEEERHRRQGLERELETLKQQLEHLEKMEVKEKVVFSESVQVEKGDTEQEIERLRGSLEAESRGKRELDAEVSRLEAKLSELEFCNSKSSKELDFLREENHKLQLERQSLQLEARRLQSEIEMAKAEAQSLRSLPTTTGADPGAQLDSRLRSLERELDDLRRLSRDKDQEIEELQRRLGSVAIKREQRENHLRRSIVVIDPDSGRELSPEEAHRAGLIDWNMFVKLRSQECDWEEISVKGPGGESSVIHDRKSGRKFSIEEALQSGRLTPAQYDRYVNKSMSIQELAVLVSGQK

Target ORF information:

RefSeq Version	XM_010799955.2
Organism	Bos taurus(cattle)
Definition	Bos taurus periplakin (PPL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010799955.2

ORF Insert Sequence:

ATGAACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCCACGGT TCGGACCCGC 
AGCGTCTCTA ACAAGGAGCT GTCGGAGCTC ATCGAGCAGT TGCAGAAGAA TGCCGACCAG 
GTGGAGAGGA ACATCGTGGA CACGGAAGCC AAGATGCAGA GAGACCTGGC CCGGCTGCAG 
GAGGGCCAGC AGCCCGAGCA CCGGGATGTG GCCCTGCAGA AGGTGTCAGA CTCTGAGAAG 
CTGCTCTACA TGCTGGAGGC GGACGCGGCC ATCGCCAAGC ACATGAAGCA CCCCCAGGGG 
GACATGATCG CCGAGGACAT CCGTCAGCTG AAGGAGCGTG TGACCAACCT CCGCGGGAAA 
CACAAGCAGA TCTACAACCT GGTGGTGAAA GAGGTGGACC CACAGGTCAA CTGGGAGGCG 
CTGGTGGAGG AAAAGCTGGA CGAGCTGAGC AGCCAGGGCT TCGGGACGGA ACTCCCGCTG 
GTAGGCCAGC AGGTGGAGCA ACACAACGTC TTTCACAACG AGGTCAAGGC CATCGGGCCC 
CACCTGGCCA AGGAGCAGAA CGGTGAACTC CAGGCCAAGT ACCAGAAACT GCTGGCGGCG 
TCACAGGCGC GGCAGCAGCA CCTGAGCTCA CTGCAGGACT ACATGCAGCG GTGCACGAAC 
GAGCTGTACT GGCTGGACCA GCAGGCCCAG GGCCGCATAC AGTATGACTG GAGTGACCGC 
AACCTCAACT ACCCCAGCCG CCGGCGCCAG TACGAGAATT TCATCCACCG GAACCTGGAG 
GCCAAAGAGG AGAGAATTAA CAAGCTGCAC AGCGAGGGTG ACCAGCTGCT CGCCGCCGAG 
CACCCCGGGA GGAACTCCAT TGAAGCTCAC ATGGAGGCTG TCCACGCAGA TTGGAAGGAA 
TACCTGAACC TGCTCATCTG CGAGGAGAGC CACCTGAAGT ACATGGAAGA CTACCACCAG 
TTCCACAAAG ACATGAAGGA TGCCCAGGAG CTGCTTCGCA AGGTGGACTC AGACCTGAAC 
CAGAAATACA GCCCTGACTT CAAGGACCGA TACCAGATTG AGCTGCTGCT GCGGGAGCTG 
GATGACCAGG AGAAGGCTCT GGACAAGTAC CAGCATGTGG TGCGGGGGCT GCAGAAGCGA 
GGGCAGCAGG TGGTGCCCCT CAAGTACCGC CGGGAGACGC CCCTCAAGCC CATCCCCGTG 
GAGGCGCTCT GTGACTTCGA GGGTGACCAG GGCCTGATCT CTCGGGGCTA CAGCTACACC 
CTGCAGAGGA ACAATGGGGA GAGCTGGGAC CTCACCGACA GTGCGGGGAA CAAGCTGACC 
GCCCCGGCCG TCTGCTTCAT GATCCCGCCC ACTGACCCCG AGGCCCTGGC TCTGGCTGAA 
AGCCTGGGCA GCCAGTACCA GAGCCTGCGG CAGAAGGCAG CTGGGAGCAG AAAGGCGCTC 
CAGGAGCAGC ATGAGGTGCT GAAGGCAGAG AGCTCTGGAG CAGATGCCTC GGACCTGCAG 
GGCCGCCAGC TGCTGGCCGG CTTGGACAAG GTGGCCAGCG ACCTGGACCG GCAGGAGAAG 
GCCATCACGG CCATCCTGCG GCCGCCGCTG GAGCAGGGCC GGGCTGTGCA GGACAGCGCC 
GAGAGGGCCA AGGACCTCAA GAACATCACC AACGAGCTGC TGCGGATCGA GCCCGAGAAG 
GCGCGCAGCA CGGCCGAGGG CGAGGCGTTC GTGCGGGCCC TCCCGGACAG CGGCAGCACC 
GCCCTGCTGA GGACGCGGGT GGAGGACACC CAGCGCAGAT ACGAGCGCCT GGTGCAGCTG 
CTGGAAGCCG CCCAGGAGAA GCTCGACATC GCCAACCGCC TGGAGAAGAG CCTGCAGCAG 
GGCCGGGAGA TGCTGGCCAC GTATGAGACC CAGCTGGCCC AGGAGGACAC GGTGCCCGAG 
AGTGGCCAGG CCCTGGACAG CAGGAGGCAG GAGCTGGCGG CCATGGCCTC CGAGCTGCAG 
GCCCGGAAGG CCCTCCTCGG CGAGGTGCAG CGGAACCTGC AGGCCGCCAA GCAGTGCTCG 
GGCTCGCTGG CCAGCCGCTT CCAGGAGCAC TGCCCGGACC TGGAGCGCCA GGAGGCCGAG 
GTGCACAAGC TGTGCCAGCG CTTCGACGGC CTCTGCCAGC AGGCGGAGCT CAGGGTCCAG 
AGCCTGCAGA GCGCCCGGGC TGAGCACGAC GCCTTCTGCA GCGGCCGTGA CCGCCTGCTT 
CAGTTCCTGT CCCACATCCC CAGCTACGAG CCCCAGGAGA CGGACGGCCT CGGCCAAGTG 
GAGACCAAGC TGAACAACCA GAAGAACCTA CTAGATGAAA TAGCAAGAAG GGAAGAGGAA 
GTGCAGAAAG TCCACACGCA CTCCCAGCAG TACCAGCAGG CTGTCAAGGA CTACGAGTTG 
GAAGCAGAGA AACTCAGGTC CCTCCTTGAC TTGGAGAATG GAAGGAGCAG CCACGTGAGC 
AAGCGGGCCA GGCTCCAGTC CCCTGCCACC AAAGTGAGGG AAGAGGAAGC AGCTCTTGCT 
GCCAAGTTTA CGGAGGTTAA TGCCATCAAC AGACAGAGAC TGCAGAACCT GGAGTTTGCA 
CTGACCCTCC TGAGACAGCT GGAGGCAGGT GCAAGCCAGG AGGCCCTGCC CGGGAGCAAG 
CCAGGCCCTG GAGCAGAGGA GACGTGGAAG ATTCAGGAGG AGCTGGATGA GGAGATGGAG 
CGGCGGCAGC AGCTGGAGCA CGAGGTCCGG AGCGCCCAGG AGGAGATCCG GGCCCTGCGG 
AGCCAGAGCC CGCAGGAGGC GGTGGTGACC AGGGAGGTGC TCAGGAAGGT GCCGGACCCA 
GCACTGGAGG AGAGCTTCCA GCAGGCGCAG CAGACCCTGG CCGAGGAGCA GCACAAGAAC 
CGGCTGCTGC AGGGGGAGGT GGAGGCGCTG CGGCTGCGGC TGCGTGCCCT GGAGGAGGCG 
GCCCGGGCCG GGGGCCAGGA GTACGTGGTC AAGGAGGTAC TGCGCATTGA GCCGGATCGG 
GCCCAGGCTG ACGAGGTCCT GAGGCTGCGG GGGGAGCTGG AGGTGCTGCG GCGGCAGAAG 
GGCGCCCGCG AGGCGGAGGC GCTTCTCCTG CAGCAGCGCG TGGCCGCCCT GGCCGAGGAG 
AAGCGGCGGG CGCGGGAGGT GGTCACAGAG AAGGAGGTGG TCAAGGTGCA CAATGACCCC 
CAGCTGGAGG CCGAGTTCCG GCAGCTGCAG GAGGACCAGC AGCGGGAGCA GGGGCTCCGG 
GAGAAGCAGG AGGAGGAGCT GGGCTTCCTG CAGGACAAGC TCAAGAGGCT GGAGAAGGAA 
CGGGCCATGG CCGAGGGCAA GATCACAGTC AAGGAGGTGC TCAAGGTGGA GAAGGACGTG 
GCGGCTGAGC GGGAGGTGGG CGCCCTCCAG CGCCGGTATG AGGATGAGGC CGCCAGGGCC 
CGTGCCAGCC AGAGGGAGAA GACCGAGCTG CTCCGGAAGA TCTGGGCCCT GGAGGAGGAG 
AACGCTAAGG TGGTTGTGCA AGAGAAGGTG CGCGAGATTG TCCGGCCTGA CCCCAAGGCT 
GAGAGTGAGG TGGCCAACCT CCGCCTGGAG CTGGTAGAGC AGGAGCGCAA GTACCGGGGG 
GCTGAGGAGC AGCTCAAGAG CTACCAGAGC GAGCTGGAGG CGCTTCGGAG GCGAGGCCCC 
CAGGTAGAGG TCAAGGAGGT GACCAAGGAG GTCATCAAGT ACACGACGGA CCCTGAGACG 
GAGAAGGAGC TCCAGAGGCT CAGGGAGGAG ATCGTGGACA AGACCCGCCT CATCGAGAGG 
TGTGACCTGG AGATCTACCA GCTGAAGCAG GAGATCCAGT CCCTCAAAGA GGCCAAGCCC 
CAGGTGCAGA CCAAGGAGGT GGTCCAGGAG ATCTTGCAGT TCCAGGAAGA CCCCCAGACC 
AAAGAGGAAG TGGCGGCTCT GAGGGCCCGG CTGGCTGAGG AGCACAAGAA GCAGGTAGAC 
CTGGAACGGG AATGCGTGTC ACAGGAGGAG AAGATCCAGG AGAAGGAGGA GGAGCTGGCC 
CGCGGGAAGG AGGTGGTGGT GCAGCAGGAG GTGGTCAGGT ACGAGGAGGA GCCGGGCCTC 
CGGGCGGAAG TGAGCGCCTT CACCGAGAGC ATCGATGCAG AACTGCGGCA GATCGACGGC 
CTCCGCGGCG AGCTGCGGCG GCTGCAGCGC CGGCGAGCCG AGCTGGAGCA CCAGCTGGAG 
GAGCTGGAGC GCGAGCGGCA GGCGCGCCGC GAGGCTGAGC TGGAGGTGCA GCACCTCAAG 
CAACAGCTGG CCCAGCTGGA GGCGCAGGAG GGCGAGGCCC ACGAGAAGGT GACCCTCACA 
CAGAAGGTGG TGCTGCAGCA GGACCCCCAG CAGGCCCGGG AGCATGCCCT CCTGGCCCTG 
CAGCTGGAGG AAGAGCGGCA CCGCCGGCAG GGCCTGGAGC GCGAGCTCGA GACCCTAAAG 
CAGCAGCTGG AGCACCTGGA GAAGATGGAG GTCAAGGAGA AGGTGGTCTT CTCCGAGAGC 
GTCCAGGTGG AGAAAGGCGA CACCGAGCAG GAGATTGAGA GGCTCAGGGG CAGTCTGGAG 
GCTGAGAGCC GAGGCAAGAG GGAGCTGGAC GCCGAGGTGA GCCGGCTGGA GGCCAAGCTG 
TCGGAGCTGG AGTTCTGCAA CTCTAAGTCA TCCAAGGAGC TGGACTTCCT GAGGGAGGAG 
AACCACAAGC TGCAGCTGGA GCGGCAGAGC CTGCAGCTCG AGGCCCGCAG GCTGCAGTCG 
GAGATCGAGA TGGCCAAGGC CGAGGCGCAG AGCCTAAGAA GCCTGCCCAC AACGACTGGA 
GCCGACCCCG GGGCGCAGCT CGACTCACGC CTGCGGTCCC TGGAGCGGGA GCTGGACGAC 
CTCAGGAGGC TCTCCAGGGA CAAAGACCAG GAGATCGAAG AGCTGCAGCG TCGCCTGGGG 
TCCGTGGCCA TCAAGCGGGA GCAGAGGGAG AACCACCTGC GGCGCTCCAT TGTGGTCATC 
GACCCTGACT CGGGCCGTGA GCTGTCCCCC GAGGAGGCCC ACCGCGCCGG CCTCATCGAC 
TGGAACATGT TTGTCAAGCT CAGGAGCCAG GAGTGCGACT GGGAGGAGAT CTCCGTCAAG 
GGCCCCGGAG GCGAGTCCTC CGTGATCCAT GACAGGAAGT CCGGCAGGAA GTTCTCCATT 
GAAGAGGCCC TGCAGAGCGG GCGGCTGACC CCCGCGCAGT ACGACCGCTA CGTCAACAAG 
AGCATGTCCA TCCAGGAGCT GGCCGTCCTG GTGTCAGGGC AGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPL ( XM_005224663.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224720.1 Bos taurus periplakin (PPL), transcript variant X3, mRNA.

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CloneID	OBa32914
Clone ID Related Accession (Same CDS sequence)	XM_005224663.3 , XM_010799955.2 , XM_005224663.4
Accession Version	XM_005224663.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	periplakin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224663.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCCACGGT TCGGACCCGC 
AGCGTCTCTA ACAAGGAGCT GTCGGAGCTC ATCGAGCAGT TGCAGAAGAA TGCCGACCAG 
GTGGAGAGGA ACATCGTGGA CACGGAAGCC AAGATGCAGA GAGACCTGGC CCGGCTGCAG 
GAGGGCCAGC AGCCCGAGCA CCGGGATGTG GCCCTGCAGA AGGTGTCAGA CTCTGAGAAG 
CTGCTCTACA TGCTGGAGGC GGACGCGGCC ATCGCCAAGC ACATGAAGCA CCCCCAGGGG 
GACATGATCG CCGAGGACAT CCGTCAGCTG AAGGAGCGTG TGACCAACCT CCGCGGGAAA 
CACAAGCAGA TCTACAACCT GGTGGTGAAA GAGGTGGACC CACAGGTCAA CTGGGAGGCG 
CTGGTGGAGG AAAAGCTGGA CGAGCTGAGC AGCCAGGGCT TCGGGACGGA ACTCCCGCTG 
GTAGGCCAGC AGGTGGAGCA ACACAACGTC TTTCACAACG AGGTCAAGGC CATCGGGCCC 
CACCTGGCCA AGGAGCAGAA CGGTGAACTC CAGGCCAAGT ACCAGAAACT GCTGGCGGCG 
TCACAGGCGC GGCAGCAGCA CCTGAGCTCA CTGCAGGACT ACATGCAGCG GTGCACGAAC 
GAGCTGTACT GGCTGGACCA GCAGGCCCAG GGCCGCATAC AGTATGACTG GAGTGACCGC 
AACCTCAACT ACCCCAGCCG CCGGCGCCAG TACGAGAATT TCATCCACCG GAACCTGGAG 
GCCAAAGAGG AGAGAATTAA CAAGCTGCAC AGCGAGGGTG ACCAGCTGCT CGCCGCCGAG 
CACCCCGGGA GGAACTCCAT TGAAGCTCAC ATGGAGGCTG TCCACGCAGA TTGGAAGGAA 
TACCTGAACC TGCTCATCTG CGAGGAGAGC CACCTGAAGT ACATGGAAGA CTACCACCAG 
TTCCACAAAG ACATGAAGGA TGCCCAGGAG CTGCTTCGCA AGGTGGACTC AGACCTGAAC 
CAGAAATACA GCCCTGACTT CAAGGACCGA TACCAGATTG AGCTGCTGCT GCGGGAGCTG 
GATGACCAGG AGAAGGCTCT GGACAAGTAC CAGCATGTGG TGCGGGGGCT GCAGAAGCGA 
GGGCAGCAGG TGGTGCCCCT CAAGTACCGC CGGGAGACGC CCCTCAAGCC CATCCCCGTG 
GAGGCGCTCT GTGACTTCGA GGGTGACCAG GGCCTGATCT CTCGGGGCTA CAGCTACACC 
CTGCAGAGGA ACAATGGGGA GAGCTGGGAC CTCACCGACA GTGCGGGGAA CAAGCTGACC 
GCCCCGGCCG TCTGCTTCAT GATCCCGCCC ACTGACCCCG AGGCCCTGGC TCTGGCTGAA 
AGCCTGGGCA GCCAGTACCA GAGCCTGCGG CAGAAGGCAG CTGGGAGCAG AAAGGCGCTC 
CAGGAGCAGC ATGAGGTGCT GAAGGCAGAG AGCTCTGGAG CAGATGCCTC GGACCTGCAG 
GGCCGCCAGC TGCTGGCCGG CTTGGACAAG GTGGCCAGCG ACCTGGACCG GCAGGAGAAG 
GCCATCACGG CCATCCTGCG GCCGCCGCTG GAGCAGGGCC GGGCTGTGCA GGACAGCGCC 
GAGAGGGCCA AGGACCTCAA GAACATCACC AACGAGCTGC TGCGGATCGA GCCCGAGAAG 
GCGCGCAGCA CGGCCGAGGG CGAGGCGTTC GTGCGGGCCC TCCCGGACAG CGGCAGCACC 
GCCCTGCTGA GGACGCGGGT GGAGGACACC CAGCGCAGAT ACGAGCGCCT GGTGCAGCTG 
CTGGAAGCCG CCCAGGAGAA GCTCGACATC GCCAACCGCC TGGAGAAGAG CCTGCAGCAG 
GGCCGGGAGA TGCTGGCCAC GTATGAGACC CAGCTGGCCC AGGAGGACAC GGTGCCCGAG 
AGTGGCCAGG CCCTGGACAG CAGGAGGCAG GAGCTGGCGG CCATGGCCTC CGAGCTGCAG 
GCCCGGAAGG CCCTCCTCGG CGAGGTGCAG CGGAACCTGC AGGCCGCCAA GCAGTGCTCG 
GGCTCGCTGG CCAGCCGCTT CCAGGAGCAC TGCCCGGACC TGGAGCGCCA GGAGGCCGAG 
GTGCACAAGC TGTGCCAGCG CTTCGACGGC CTCTGCCAGC AGGCGGAGCT CAGGGTCCAG 
AGCCTGCAGA GCGCCCGGGC TGAGCACGAC GCCTTCTGCA GCGGCCGTGA CCGCCTGCTT 
CAGTTCCTGT CCCACATCCC CAGCTACGAG CCCCAGGAGA CGGACGGCCT CGGCCAAGTG 
GAGACCAAGC TGAACAACCA GAAGAACCTA CTAGATGAAA TAGCAAGAAG GGAAGAGGAA 
GTGCAGAAAG TCCACACGCA CTCCCAGCAG TACCAGCAGG CTGTCAAGGA CTACGAGTTG 
GAAGCAGAGA AACTCAGGTC CCTCCTTGAC TTGGAGAATG GAAGGAGCAG CCACGTGAGC 
AAGCGGGCCA GGCTCCAGTC CCCTGCCACC AAAGTGAGGG AAGAGGAAGC AGCTCTTGCT 
GCCAAGTTTA CGGAGGTTAA TGCCATCAAC AGACAGAGAC TGCAGAACCT GGAGTTTGCA 
CTGACCCTCC TGAGACAGCT GGAGGCAGGT GCAAGCCAGG AGGCCCTGCC CGGGAGCAAG 
CCAGGCCCTG GAGCAGAGGA GACGTGGAAG ATTCAGGAGG AGCTGGATGA GGAGATGGAG 
CGGCGGCAGC AGCTGGAGCA CGAGGTCCGG AGCGCCCAGG AGGAGATCCG GGCCCTGCGG 
AGCCAGAGCC CGCAGGAGGC GGTGGTGACC AGGGAGGTGC TCAGGAAGGT GCCGGACCCA 
GCACTGGAGG AGAGCTTCCA GCAGGCGCAG CAGACCCTGG CCGAGGAGCA GCACAAGAAC 
CGGCTGCTGC AGGGGGAGGT GGAGGCGCTG CGGCTGCGGC TGCGTGCCCT GGAGGAGGCG 
GCCCGGGCCG GGGGCCAGGA GTACGTGGTC AAGGAGGTAC TGCGCATTGA GCCGGATCGG 
GCCCAGGCTG ACGAGGTCCT GAGGCTGCGG GGGGAGCTGG AGGTGCTGCG GCGGCAGAAG 
GGCGCCCGCG AGGCGGAGGC GCTTCTCCTG CAGCAGCGCG TGGCCGCCCT GGCCGAGGAG 
AAGCGGCGGG CGCGGGAGGT GGTCACAGAG AAGGAGGTGG TCAAGGTGCA CAATGACCCC 
CAGCTGGAGG CCGAGTTCCG GCAGCTGCAG GAGGACCAGC AGCGGGAGCA GGGGCTCCGG 
GAGAAGCAGG AGGAGGAGCT GGGCTTCCTG CAGGACAAGC TCAAGAGGCT GGAGAAGGAA 
CGGGCCATGG CCGAGGGCAA GATCACAGTC AAGGAGGTGC TCAAGGTGGA GAAGGACGTG 
GCGGCTGAGC GGGAGGTGGG CGCCCTCCAG CGCCGGTATG AGGATGAGGC CGCCAGGGCC 
CGTGCCAGCC AGAGGGAGAA GACCGAGCTG CTCCGGAAGA TCTGGGCCCT GGAGGAGGAG 
AACGCTAAGG TGGTTGTGCA AGAGAAGGTG CGCGAGATTG TCCGGCCTGA CCCCAAGGCT 
GAGAGTGAGG TGGCCAACCT CCGCCTGGAG CTGGTAGAGC AGGAGCGCAA GTACCGGGGG 
GCTGAGGAGC AGCTCAAGAG CTACCAGAGC GAGCTGGAGG CGCTTCGGAG GCGAGGCCCC 
CAGGTAGAGG TCAAGGAGGT GACCAAGGAG GTCATCAAGT ACACGACGGA CCCTGAGACG 
GAGAAGGAGC TCCAGAGGCT CAGGGAGGAG ATCGTGGACA AGACCCGCCT CATCGAGAGG 
TGTGACCTGG AGATCTACCA GCTGAAGCAG GAGATCCAGT CCCTCAAAGA GGCCAAGCCC 
CAGGTGCAGA CCAAGGAGGT GGTCCAGGAG ATCTTGCAGT TCCAGGAAGA CCCCCAGACC 
AAAGAGGAAG TGGCGGCTCT GAGGGCCCGG CTGGCTGAGG AGCACAAGAA GCAGGTAGAC 
CTGGAACGGG AATGCGTGTC ACAGGAGGAG AAGATCCAGG AGAAGGAGGA GGAGCTGGCC 
CGCGGGAAGG AGGTGGTGGT GCAGCAGGAG GTGGTCAGGT ACGAGGAGGA GCCGGGCCTC 
CGGGCGGAAG TGAGCGCCTT CACCGAGAGC ATCGATGCAG AACTGCGGCA GATCGACGGC 
CTCCGCGGCG AGCTGCGGCG GCTGCAGCGC CGGCGAGCCG AGCTGGAGCA CCAGCTGGAG 
GAGCTGGAGC GCGAGCGGCA GGCGCGCCGC GAGGCTGAGC TGGAGGTGCA GCACCTCAAG 
CAACAGCTGG CCCAGCTGGA GGCGCAGGAG GGCGAGGCCC ACGAGAAGGT GACCCTCACA 
CAGAAGGTGG TGCTGCAGCA GGACCCCCAG CAGGCCCGGG AGCATGCCCT CCTGGCCCTG 
CAGCTGGAGG AAGAGCGGCA CCGCCGGCAG GGCCTGGAGC GCGAGCTCGA GACCCTAAAG 
CAGCAGCTGG AGCACCTGGA GAAGATGGAG GTCAAGGAGA AGGTGGTCTT CTCCGAGAGC 
GTCCAGGTGG AGAAAGGCGA CACCGAGCAG GAGATTGAGA GGCTCAGGGG CAGTCTGGAG 
GCTGAGAGCC GAGGCAAGAG GGAGCTGGAC GCCGAGGTGA GCCGGCTGGA GGCCAAGCTG 
TCGGAGCTGG AGTTCTGCAA CTCTAAGTCA TCCAAGGAGC TGGACTTCCT GAGGGAGGAG 
AACCACAAGC TGCAGCTGGA GCGGCAGAGC CTGCAGCTCG AGGCCCGCAG GCTGCAGTCG 
GAGATCGAGA TGGCCAAGGC CGAGGCGCAG AGCCTAAGAA GCCTGCCCAC AACGACTGGA 
GCCGACCCCG GGGCGCAGCT CGACTCACGC CTGCGGTCCC TGGAGCGGGA GCTGGACGAC 
CTCAGGAGGC TCTCCAGGGA CAAAGACCAG GAGATCGAAG AGCTGCAGCG TCGCCTGGGG 
TCCGTGGCCA TCAAGCGGGA GCAGAGGGAG AACCACCTGC GGCGCTCCAT TGTGGTCATC 
GACCCTGACT CGGGCCGTGA GCTGTCCCCC GAGGAGGCCC ACCGCGCCGG CCTCATCGAC 
TGGAACATGT TTGTCAAGCT CAGGAGCCAG GAGTGCGACT GGGAGGAGAT CTCCGTCAAG 
GGCCCCGGAG GCGAGTCCTC CGTGATCCAT GACAGGAAGT CCGGCAGGAA GTTCTCCATT 
GAAGAGGCCC TGCAGAGCGG GCGGCTGACC CCCGCGCAGT ACGACCGCTA CGTCAACAAG 
AGCATGTCCA TCCAGGAGCT GGCCGTCCTG GTGTCAGGGC AGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224720.1
CDS	225..5492
Translation	MNSLFRKRNKGKYSPTVRTRSVSNKELSELIEQLQKNADQVERNIVDTEAKMQRDLARLQEGQQPEHRDVALQKVSDSEKLLYMLEADAAIAKHMKHPQGDMIAEDIRQLKERVTNLRGKHKQIYNLVVKEVDPQVNWEALVEEKLDELSSQGFGTELPLVGQQVEQHNVFHNEVKAIGPHLAKEQNGELQAKYQKLLAASQARQQHLSSLQDYMQRCTNELYWLDQQAQGRIQYDWSDRNLNYPSRRRQYENFIHRNLEAKEERINKLHSEGDQLLAAEHPGRNSIEAHMEAVHADWKEYLNLLICEESHLKYMEDYHQFHKDMKDAQELLRKVDSDLNQKYSPDFKDRYQIELLLRELDDQEKALDKYQHVVRGLQKRGQQVVPLKYRRETPLKPIPVEALCDFEGDQGLISRGYSYTLQRNNGESWDLTDSAGNKLTAPAVCFMIPPTDPEALALAESLGSQYQSLRQKAAGSRKALQEQHEVLKAESSGADASDLQGRQLLAGLDKVASDLDRQEKAITAILRPPLEQGRAVQDSAERAKDLKNITNELLRIEPEKARSTAEGEAFVRALPDSGSTALLRTRVEDTQRRYERLVQLLEAAQEKLDIANRLEKSLQQGREMLATYETQLAQEDTVPESGQALDSRRQELAAMASELQARKALLGEVQRNLQAAKQCSGSLASRFQEHCPDLERQEAEVHKLCQRFDGLCQQAELRVQSLQSARAEHDAFCSGRDRLLQFLSHIPSYEPQETDGLGQVETKLNNQKNLLDEIARREEEVQKVHTHSQQYQQAVKDYELEAEKLRSLLDLENGRSSHVSKRARLQSPATKVREEEAALAAKFTEVNAINRQRLQNLEFALTLLRQLEAGASQEALPGSKPGPGAEETWKIQEELDEEMERRQQLEHEVRSAQEEIRALRSQSPQEAVVTREVLRKVPDPALEESFQQAQQTLAEEQHKNRLLQGEVEALRLRLRALEEAARAGGQEYVVKEVLRIEPDRAQADEVLRLRGELEVLRRQKGAREAEALLLQQRVAALAEEKRRAREVVTEKEVVKVHNDPQLEAEFRQLQEDQQREQGLREKQEEELGFLQDKLKRLEKERAMAEGKITVKEVLKVEKDVAAEREVGALQRRYEDEAARARASQREKTELLRKIWALEEENAKVVVQEKVREIVRPDPKAESEVANLRLELVEQERKYRGAEEQLKSYQSELEALRRRGPQVEVKEVTKEVIKYTTDPETEKELQRLREEIVDKTRLIERCDLEIYQLKQEIQSLKEAKPQVQTKEVVQEILQFQEDPQTKEEVAALRARLAEEHKKQVDLERECVSQEEKIQEKEEELARGKEVVVQQEVVRYEEEPGLRAEVSAFTESIDAELRQIDGLRGELRRLQRRRAELEHQLEELERERQARREAELEVQHLKQQLAQLEAQEGEAHEKVTLTQKVVLQQDPQQAREHALLALQLEEERHRRQGLERELETLKQQLEHLEKMEVKEKVVFSESVQVEKGDTEQEIERLRGSLEAESRGKRELDAEVSRLEAKLSELEFCNSKSSKELDFLREENHKLQLERQSLQLEARRLQSEIEMAKAEAQSLRSLPTTTGADPGAQLDSRLRSLERELDDLRRLSRDKDQEIEELQRRLGSVAIKREQRENHLRRSIVVIDPDSGRELSPEEAHRAGLIDWNMFVKLRSQECDWEEISVKGPGGESSVIHDRKSGRKFSIEEALQSGRLTPAQYDRYVNKSMSIQELAVLVSGQK

Target ORF information:

RefSeq Version	XM_005224663.4
Organism	Bos taurus(cattle)
Definition	Bos taurus periplakin (PPL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224663.4

ORF Insert Sequence:

ATGAACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCCACGGT TCGGACCCGC 
AGCGTCTCTA ACAAGGAGCT GTCGGAGCTC ATCGAGCAGT TGCAGAAGAA TGCCGACCAG 
GTGGAGAGGA ACATCGTGGA CACGGAAGCC AAGATGCAGA GAGACCTGGC CCGGCTGCAG 
GAGGGCCAGC AGCCCGAGCA CCGGGATGTG GCCCTGCAGA AGGTGTCAGA CTCTGAGAAG 
CTGCTCTACA TGCTGGAGGC GGACGCGGCC ATCGCCAAGC ACATGAAGCA CCCCCAGGGG 
GACATGATCG CCGAGGACAT CCGTCAGCTG AAGGAGCGTG TGACCAACCT CCGCGGGAAA 
CACAAGCAGA TCTACAACCT GGTGGTGAAA GAGGTGGACC CACAGGTCAA CTGGGAGGCG 
CTGGTGGAGG AAAAGCTGGA CGAGCTGAGC AGCCAGGGCT TCGGGACGGA ACTCCCGCTG 
GTAGGCCAGC AGGTGGAGCA ACACAACGTC TTTCACAACG AGGTCAAGGC CATCGGGCCC 
CACCTGGCCA AGGAGCAGAA CGGTGAACTC CAGGCCAAGT ACCAGAAACT GCTGGCGGCG 
TCACAGGCGC GGCAGCAGCA CCTGAGCTCA CTGCAGGACT ACATGCAGCG GTGCACGAAC 
GAGCTGTACT GGCTGGACCA GCAGGCCCAG GGCCGCATAC AGTATGACTG GAGTGACCGC 
AACCTCAACT ACCCCAGCCG CCGGCGCCAG TACGAGAATT TCATCCACCG GAACCTGGAG 
GCCAAAGAGG AGAGAATTAA CAAGCTGCAC AGCGAGGGTG ACCAGCTGCT CGCCGCCGAG 
CACCCCGGGA GGAACTCCAT TGAAGCTCAC ATGGAGGCTG TCCACGCAGA TTGGAAGGAA 
TACCTGAACC TGCTCATCTG CGAGGAGAGC CACCTGAAGT ACATGGAAGA CTACCACCAG 
TTCCACAAAG ACATGAAGGA TGCCCAGGAG CTGCTTCGCA AGGTGGACTC AGACCTGAAC 
CAGAAATACA GCCCTGACTT CAAGGACCGA TACCAGATTG AGCTGCTGCT GCGGGAGCTG 
GATGACCAGG AGAAGGCTCT GGACAAGTAC CAGCATGTGG TGCGGGGGCT GCAGAAGCGA 
GGGCAGCAGG TGGTGCCCCT CAAGTACCGC CGGGAGACGC CCCTCAAGCC CATCCCCGTG 
GAGGCGCTCT GTGACTTCGA GGGTGACCAG GGCCTGATCT CTCGGGGCTA CAGCTACACC 
CTGCAGAGGA ACAATGGGGA GAGCTGGGAC CTCACCGACA GTGCGGGGAA CAAGCTGACC 
GCCCCGGCCG TCTGCTTCAT GATCCCGCCC ACTGACCCCG AGGCCCTGGC TCTGGCTGAA 
AGCCTGGGCA GCCAGTACCA GAGCCTGCGG CAGAAGGCAG CTGGGAGCAG AAAGGCGCTC 
CAGGAGCAGC ATGAGGTGCT GAAGGCAGAG AGCTCTGGAG CAGATGCCTC GGACCTGCAG 
GGCCGCCAGC TGCTGGCCGG CTTGGACAAG GTGGCCAGCG ACCTGGACCG GCAGGAGAAG 
GCCATCACGG CCATCCTGCG GCCGCCGCTG GAGCAGGGCC GGGCTGTGCA GGACAGCGCC 
GAGAGGGCCA AGGACCTCAA GAACATCACC AACGAGCTGC TGCGGATCGA GCCCGAGAAG 
GCGCGCAGCA CGGCCGAGGG CGAGGCGTTC GTGCGGGCCC TCCCGGACAG CGGCAGCACC 
GCCCTGCTGA GGACGCGGGT GGAGGACACC CAGCGCAGAT ACGAGCGCCT GGTGCAGCTG 
CTGGAAGCCG CCCAGGAGAA GCTCGACATC GCCAACCGCC TGGAGAAGAG CCTGCAGCAG 
GGCCGGGAGA TGCTGGCCAC GTATGAGACC CAGCTGGCCC AGGAGGACAC GGTGCCCGAG 
AGTGGCCAGG CCCTGGACAG CAGGAGGCAG GAGCTGGCGG CCATGGCCTC CGAGCTGCAG 
GCCCGGAAGG CCCTCCTCGG CGAGGTGCAG CGGAACCTGC AGGCCGCCAA GCAGTGCTCG 
GGCTCGCTGG CCAGCCGCTT CCAGGAGCAC TGCCCGGACC TGGAGCGCCA GGAGGCCGAG 
GTGCACAAGC TGTGCCAGCG CTTCGACGGC CTCTGCCAGC AGGCGGAGCT CAGGGTCCAG 
AGCCTGCAGA GCGCCCGGGC TGAGCACGAC GCCTTCTGCA GCGGCCGTGA CCGCCTGCTT 
CAGTTCCTGT CCCACATCCC CAGCTACGAG CCCCAGGAGA CGGACGGCCT CGGCCAAGTG 
GAGACCAAGC TGAACAACCA GAAGAACCTA CTAGATGAAA TAGCAAGAAG GGAAGAGGAA 
GTGCAGAAAG TCCACACGCA CTCCCAGCAG TACCAGCAGG CTGTCAAGGA CTACGAGTTG 
GAAGCAGAGA AACTCAGGTC CCTCCTTGAC TTGGAGAATG GAAGGAGCAG CCACGTGAGC 
AAGCGGGCCA GGCTCCAGTC CCCTGCCACC AAAGTGAGGG AAGAGGAAGC AGCTCTTGCT 
GCCAAGTTTA CGGAGGTTAA TGCCATCAAC AGACAGAGAC TGCAGAACCT GGAGTTTGCA 
CTGACCCTCC TGAGACAGCT GGAGGCAGGT GCAAGCCAGG AGGCCCTGCC CGGGAGCAAG 
CCAGGCCCTG GAGCAGAGGA GACGTGGAAG ATTCAGGAGG AGCTGGATGA GGAGATGGAG 
CGGCGGCAGC AGCTGGAGCA CGAGGTCCGG AGCGCCCAGG AGGAGATCCG GGCCCTGCGG 
AGCCAGAGCC CGCAGGAGGC GGTGGTGACC AGGGAGGTGC TCAGGAAGGT GCCGGACCCA 
GCACTGGAGG AGAGCTTCCA GCAGGCGCAG CAGACCCTGG CCGAGGAGCA GCACAAGAAC 
CGGCTGCTGC AGGGGGAGGT GGAGGCGCTG CGGCTGCGGC TGCGTGCCCT GGAGGAGGCG 
GCCCGGGCCG GGGGCCAGGA GTACGTGGTC AAGGAGGTAC TGCGCATTGA GCCGGATCGG 
GCCCAGGCTG ACGAGGTCCT GAGGCTGCGG GGGGAGCTGG AGGTGCTGCG GCGGCAGAAG 
GGCGCCCGCG AGGCGGAGGC GCTTCTCCTG CAGCAGCGCG TGGCCGCCCT GGCCGAGGAG 
AAGCGGCGGG CGCGGGAGGT GGTCACAGAG AAGGAGGTGG TCAAGGTGCA CAATGACCCC 
CAGCTGGAGG CCGAGTTCCG GCAGCTGCAG GAGGACCAGC AGCGGGAGCA GGGGCTCCGG 
GAGAAGCAGG AGGAGGAGCT GGGCTTCCTG CAGGACAAGC TCAAGAGGCT GGAGAAGGAA 
CGGGCCATGG CCGAGGGCAA GATCACAGTC AAGGAGGTGC TCAAGGTGGA GAAGGACGTG 
GCGGCTGAGC GGGAGGTGGG CGCCCTCCAG CGCCGGTATG AGGATGAGGC CGCCAGGGCC 
CGTGCCAGCC AGAGGGAGAA GACCGAGCTG CTCCGGAAGA TCTGGGCCCT GGAGGAGGAG 
AACGCTAAGG TGGTTGTGCA AGAGAAGGTG CGCGAGATTG TCCGGCCTGA CCCCAAGGCT 
GAGAGTGAGG TGGCCAACCT CCGCCTGGAG CTGGTAGAGC AGGAGCGCAA GTACCGGGGG 
GCTGAGGAGC AGCTCAAGAG CTACCAGAGC GAGCTGGAGG CGCTTCGGAG GCGAGGCCCC 
CAGGTAGAGG TCAAGGAGGT GACCAAGGAG GTCATCAAGT ACACGACGGA CCCTGAGACG 
GAGAAGGAGC TCCAGAGGCT CAGGGAGGAG ATCGTGGACA AGACCCGCCT CATCGAGAGG 
TGTGACCTGG AGATCTACCA GCTGAAGCAG GAGATCCAGT CCCTCAAAGA GGCCAAGCCC 
CAGGTGCAGA CCAAGGAGGT GGTCCAGGAG ATCTTGCAGT TCCAGGAAGA CCCCCAGACC 
AAAGAGGAAG TGGCGGCTCT GAGGGCCCGG CTGGCTGAGG AGCACAAGAA GCAGGTAGAC 
CTGGAACGGG AATGCGTGTC ACAGGAGGAG AAGATCCAGG AGAAGGAGGA GGAGCTGGCC 
CGCGGGAAGG AGGTGGTGGT GCAGCAGGAG GTGGTCAGGT ACGAGGAGGA GCCGGGCCTC 
CGGGCGGAAG TGAGCGCCTT CACCGAGAGC ATCGATGCAG AACTGCGGCA GATCGACGGC 
CTCCGCGGCG AGCTGCGGCG GCTGCAGCGC CGGCGAGCCG AGCTGGAGCA CCAGCTGGAG 
GAGCTGGAGC GCGAGCGGCA GGCGCGCCGC GAGGCTGAGC TGGAGGTGCA GCACCTCAAG 
CAACAGCTGG CCCAGCTGGA GGCGCAGGAG GGCGAGGCCC ACGAGAAGGT GACCCTCACA 
CAGAAGGTGG TGCTGCAGCA GGACCCCCAG CAGGCCCGGG AGCATGCCCT CCTGGCCCTG 
CAGCTGGAGG AAGAGCGGCA CCGCCGGCAG GGCCTGGAGC GCGAGCTCGA GACCCTAAAG 
CAGCAGCTGG AGCACCTGGA GAAGATGGAG GTCAAGGAGA AGGTGGTCTT CTCCGAGAGC 
GTCCAGGTGG AGAAAGGCGA CACCGAGCAG GAGATTGAGA GGCTCAGGGG CAGTCTGGAG 
GCTGAGAGCC GAGGCAAGAG GGAGCTGGAC GCCGAGGTGA GCCGGCTGGA GGCCAAGCTG 
TCGGAGCTGG AGTTCTGCAA CTCTAAGTCA TCCAAGGAGC TGGACTTCCT GAGGGAGGAG 
AACCACAAGC TGCAGCTGGA GCGGCAGAGC CTGCAGCTCG AGGCCCGCAG GCTGCAGTCG 
GAGATCGAGA TGGCCAAGGC CGAGGCGCAG AGCCTAAGAA GCCTGCCCAC AACGACTGGA 
GCCGACCCCG GGGCGCAGCT CGACTCACGC CTGCGGTCCC TGGAGCGGGA GCTGGACGAC 
CTCAGGAGGC TCTCCAGGGA CAAAGACCAG GAGATCGAAG AGCTGCAGCG TCGCCTGGGG 
TCCGTGGCCA TCAAGCGGGA GCAGAGGGAG AACCACCTGC GGCGCTCCAT TGTGGTCATC 
GACCCTGACT CGGGCCGTGA GCTGTCCCCC GAGGAGGCCC ACCGCGCCGG CCTCATCGAC 
TGGAACATGT TTGTCAAGCT CAGGAGCCAG GAGTGCGACT GGGAGGAGAT CTCCGTCAAG 
GGCCCCGGAG GCGAGTCCTC CGTGATCCAT GACAGGAAGT CCGGCAGGAA GTTCTCCATT 
GAAGAGGCCC TGCAGAGCGG GCGGCTGACC CCCGCGCAGT ACGACCGCTA CGTCAACAAG 
AGCATGTCCA TCCAGGAGCT GGCCGTCCTG GTGTCAGGGC AGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PPL
Entrez Gene ID	522886
Full Name	periplakin
General protein information	Preferred Names periplakin Names periplakin
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Pan troglodytes (chimpanzee)	PPL	XP_003314985.2
Gallus gallus (chicken)	PPL	XP_414712.3
Homo sapiens (human)	PPL	NP_002696.3
	PPL	XP_001098863.2
Canis lupus familiaris (dog)	PPL	XP_005621642.1
Xenopus tropicalis (tropical clawed frog)	ppl	XP_002941094.2
Bos taurus (cattle)	PPL	XP_005224719.1
Mus musculus (house mouse)	Ppl	NP_032935.2
Rattus norvegicus (Norway rat)	Ppl	NP_001100446.1
Danio rerio (zebrafish)	ppl	NP_001131138.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PPL cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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