PPL cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPL
Entrez Gene ID	5493
Full Name	periplakin
General protein information	Preferred Names periplakin Names periplakin 190 kDa paraneoplastic pemphigus antigen 195 kDa cornified envelope precursor protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p13.3
Summary	The protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling. [provided by RefSeq, Jul 2008].
Disorder MIM:	602871

mRNA and Protein(s)

mRNA	Protein	Name
XM_017023374.1	XP_016878863.1	periplakin isoform X1
NM_002705.4	NP_002696.3	periplakin
XM_006720902.4	XP_006720965.1	periplakin isoform X3
XM_017023375.1	XP_016878864.1	periplakin isoform X2
XM_006720902.3	XP_006720965.1	periplakin isoform X3
NM_002705.5	NP_002696.4	periplakin
XM_017023375.2	XP_016878864.1	periplakin isoform X2
XM_017023374.2	XP_016878863.1	periplakin isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PPL	XP_003314985.2
Gallus gallus (chicken)	PPL	XP_414712.3
Homo sapiens (human)	PPL	NP_002696.3
	PPL	XP_001098863.2
Canis lupus familiaris (dog)	PPL	XP_005621642.1
Xenopus tropicalis (tropical clawed frog)	ppl	XP_002941094.2
Bos taurus (cattle)	PPL	XP_005224719.1
Mus musculus (house mouse)	Ppl	NP_032935.2
Rattus norvegicus (Norway rat)	Ppl	NP_001100446.1
Danio rerio (zebrafish)	ppl	NP_001131138.1

Gene Ontology
Bibliography

Related articles in PubMed

Loss of periplakin expression is associated with the tumorigenesis of colorectal carcinoma.
Li X, Zhang G, Wang Y, Elgehama A, Sun Y, Li L, Gu Y, Guo W, Xu Q
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie87366-374(2017 Mar)

Perip7lakin is a target for autoimmunity in asthma.
Taill? C, Grootenboer-Mignot S, Estellat C, Roy C, Ly Ka So S, Pretolani M, Aubier M, Crestani B, Chollet-Martin S
Respiratory research17(1)126(2016 Oct)

Activation of human ?? T cells by cytosolic interactions of BTN3A1 with soluble phosphoantigens and the cytoskeletal adaptor periplakin.
Rhodes DA, Chen HC, Price AJ, Keeble AH, Davey MS, James LC, Eberl M, Trowsdale J
Journal of immunology (Baltimore, Md. : 1950)194(5)2390-8(2015 Mar)

Serum periplakin as a potential biomarker for urothelial carcinoma of the urinary bladder.
Matsumoto K, Ikeda M, Matsumoto T, Nagashio R, Nishimori T, Tomonaga T, Nomura F, Sato Y, Kitasato H, Iwamura M
Asian Pacific journal of cancer prevention : APJCP15(22)9927-31(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PPL overexpression inhibited the growth of colon cancer.
Title: Loss of periplakin expression is associated with the tumorigenesis of colorectal carcinoma.

Letter: suggest periplakin autoimmunity may be involved in asthma pathogenesis.
Title: Perip7lakin is a target for autoimmunity in asthma.

Human gamma-delta T cells are activated by cytosolic interactions of BTN3A1 with soluble phosphoantigens and the cytoskeletal adaptor periplakin.
Title: Activation of human ?? T cells by cytosolic interactions of BTN3A1 with soluble phosphoantigens and the cytoskeletal adaptor periplakin.

Loss of periplakin expression is associated with pathological stage and cancer-specific survival in patients with urothelial carcinoma of the urinary bladder.
Title: Loss of periplakin expression is associated with pathological stage and cancer-specific survival in patients with urothelial carcinoma of the urinary bladder.

Low serum periplakin is associated with urothelial carcinoma.
Title: Serum periplakin as a potential biomarker for urothelial carcinoma of the urinary bladder.

The following PPL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13532	NM_002705.4 Latest version!	Homo sapiens periplakin (PPL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu107375	NM_002705.5 Latest version!	Homo sapiens periplakin (PPL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu48890	XM_006720902.4 Latest version!	Homo sapiens periplakin (PPL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu86120	XM_017023374.2 Latest version!	Homo sapiens periplakin (PPL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu86121	XM_017023375.2 Latest version!	Homo sapiens periplakin (PPL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OHu48890	XM_006720902.3	Homo sapiens periplakin (PPL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu86121	XM_017023375.1	Homo sapiens periplakin (PPL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu86120	XM_017023374.1	Homo sapiens periplakin (PPL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPL ( NM_002705.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002696.3 Homo sapiens periplakin (PPL), mRNA.

Price & Availability↑

CloneID	OHu13532
Clone ID Related Accession (Same CDS sequence)	NM_002705.4
Accession Version	NM_002705.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	periplakin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF001691.1, AC027687.7, AF013717.1, BC114620.1, AW582622.1 and BM673980.1. On Dec 1, 2006 this sequence version replaced NM_002705.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF001691.1, AF013717.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCCACTGT GCAGACCCGG 
AGCATCTCTA ACAAGGAGCT CTCGGAGCTG ATCGAGCAGC TGCAGAAGAA TGCCGACCAG 
GTGGAGAAGA ACATCGTGGA CACAGAGGCC AAGATGCAGA GTGACCTGGC TCGGCTGCAG 
GAGGGTCGGC AGCCTGAGCA CCGGGACGTG ACCCTGCAGA AGGTGTTGGA CTCTGAGAAG 
CTGCTCTATG TGCTAGAGGC GGATGCGGCC ATTGCCAAGC ACATGAAGCA CCCACAGGGG 
GACATGATCG CCGAGGATAT CCGCCAGCTG AAGGAGCGTG TGACCAACCT GCGCGGGAAA 
CACAAGCAGA TCTACAGGCT GGCGGTGAAG GAAGTGGATC CACAGGTCAA CTGGGCGGCA 
CTGGTGGAGG AGAAGCTGGA CAAGCTGAAC AACCAGAGCT TTGGGACTGA CCTGCCGCTG 
GTGGACCACC AAGTGGAGGA GCATAACATC TTCCACAATG AGGTCAAGGC CATCGGGCCC 
CACCTGGCCA AGGACGGGGA CAAGGAGCAG AACAGCGAAC TCCGGGCCAA GTACCAGAAA 
CTGCTGGCAG CATCACAGGC CCGGCAGCAG CACCTGAGTT CGCTGCAGGA CTACATGCAG 
CGCTGCACCA ATGAGCTGTA CTGGCTGGAC CAGCAGGCCA AGGGCCGCAT GCAGTACGAC 
TGGAGTGACC GCAACCTCGA CTACCCCAGC CGCCGGCGCC AGTATGAGAA TTTCATCAAC 
CGGAACCTGG AGGCCAAAGA GGAGAGAATC AACAAACTGC ACAGCGAGGG CGACCAGCTG 
CTGGCGGCCG AGCACCCCGG GAGGAACTCC ATTGAGGCGC ACATGGAGGC TGTGCACGCA 
GACTGGAAGG AGTACCTGAA CCTGCTCATC TGCGAGGAGA GCCACCTCAA GTACATGGAG 
GACTACCACC AGTTTCACGA AGACGTGAAG GACGCTCAGG AGCTGCTGCG CAAGGTGGAC 
TCGGACCTGA ACCAGAAGTA TGGCCCTGAC TTCAAGGACC GGTACCAGAT TGAGCTGCTG 
CTGCGGGAGC TGGATGACCA GGAGAAGGTG CTGGACAAGT ATGAGGACGT GGTGCAGGGG 
CTGCAGAAGC GAGGCCAGCA GGTGGTGCCC CTCAAGTACC GCCGGGAGAC TCCGCTCAAG 
CCCATCCCCG TGGAGGCACT CTGTGACTTT GAGGGGGAGC AGGGCCTGAT CTCGCGGGGC 
TACAGCTACA CCCTGCAGAA GAACAACGGG GAGAGCTGGG AGCTCATGGA CAGCGCTGGG 
AACAAGCTGA TTGCTCCGGC CGTGTGTTTT GTGATCCCCC CCACAGACCC TGAGGCCCTG 
GCTCTGGCTG ACAGCCTGGG CAGCCAGTAC CGGAGCGTGC GGCAGAAGGC AGCTGGGAGC 
AAACGCACGC TGCAGCAGCG GTATGAGGTG CTGAAGACCG AGAATCCCGG AGATGCCTCT 
GACCTACAGG GGCGGCAGCT GCTGGCTGGC TTGGACAAGG TGGCCAGCGA CCTGGACCGG 
CAGGAGAAGG CCATCACAGG GATCCTGCGG CCACCACTGG AGCAAGGCCG GGCTGTGCAG 
GACAGTGCCG AGCGGGCCAA GGACCTCAAG AACATCACCA ACGAGCTACT GCGGATTGAA 
CCTGAGAAGA CGCGGAGCAC GGCTGAGGGC GAAGCCTTCA TCCAGGCCCT CCCAGGCAGT 
GGCACCACAC CCCTGCTGAG GACCCGGGTG GAGGACACCA ACCGGAAATA CGAGCACCTC 
CTGCAGCTGC TGGACTTGGC CCAGGAGAAG GTTGATGTGG CCAACCGCCT GGAGAAGAGC 
CTGCAGCAGA GCTGGGAGTT GCTGGCCACA CACGAGAACC ATCTGAATCA GGATGACACA 
GTGCCTGAGA GCAGCCGTGT CCTGGACAGC AAGGGGCAGG AGCTGGCGGC CATGGCCTGT 
GAGTTACAGG CCCAGAAGTC CCTCCTGGGT GAGGTGGAGC AGAACTTGCA GGCGGCCAAG 
CAGTGCTCGA GCACACTGGC CAGCCGCTTC CAGGAGCACT GTCCGGACCT GGAGCGCCAG 
GAGGCCGAGG TGCACAAGCT GGGCCAGCGT TTCAACAACC TGCGCCAGCA GGTGGAACGC 
AGGGCGCAGA GCCTACAGAG CGCCAAGGCA GCCTACGAGC ACTTCCACCG CGGCCATGAC 
CACGTGCTGC AGTTCCTAGT CAGCATCCCC AGTTACGAGC CCCAGGAGAC AGACAGCCTC 
AGCCAGATGG AGACCAAGCT GAAGAACCAG AAGAACCTGC TAGATGAGAT AGCAAGTAGG 
GAGCAGGAAG TACAGAAGAT CTGTGCCAAT TCCCAGCAGT ACCAGCAAGC TGTAAAGGAC 
TATGAGTTAG AAGCAGAAAA ACTAAGGTCT CTTCTCGACT TGGAGAATGG AAGGAGCAGC 
CACGTGAGCA AGAGAGCCAG GCTCCAATCT CCTGCCACCA AAGTGAAGGA AGAGGAAGCA 
GCACTTGCCG CCAAGTTCAC TGAAGTTTAT GCCATCAACA GACAGAGGCT GCAGAATCTG 
GAGTTTGCTC TGAATCTCCT CAGACAGCAG CCGGAAGTAG AAGTGACCCA TGAGACCCTG 
CAAAGGAATA GGCCGGACTC TGGAGTGGAG GAGGCGTGGA AGATCAGGAA GGAACTGGAT 
GAGGAGACTG AGCGGAGGCG GCAGCTGGAG AACGAGGTCA AGAGCACCCA GGAAGAAATC 
TGGACCTTGA GGAATCAGGG GCCTCAGGAA TCGGTGGTGA GGAAGGAGGT GCTCAAGAAG 
GTGCCGGATC CCGTGCTGGA GGAGAGCTTC CAGCAGCTGC AGCGGACGCT GGCAGAGGAG 
CAGCACAAGA ACCAGCTGCT GCAGGAGGAG CTGGAGGCAC TGCAGCTGCA GCTGCGTGCC 
CTGGAGCAGG AGACCAGAGA CGGGGGGCAG GAGTACGTGG TCAAGGAGGT CCTGCGCATC 
GAGCCTGACA GGGCCCAGGC GGATGAGGTC TTGCAGCTGC GGGAGGAGCT GGAGGCACTG 
AGGCGGCAGA AGGGCGCCCG GGAGGCAGAG GTGCTCCTCC TGCAGCAGCG TGTGGCCGCC 
CTGGCTGAAG AGAAGAGCCG GGCGCAGGAG AAGGTCACAG AGAAAGAGGT GGTGAAACTG 
CAGAATGACC CCCAGCTGGA GGCAGAGTAC CAGCAGCTGC AGGAGGACCA CCAGCGCCAG 
GACCAGCTCA GGGAGAAGCA GGAGGAGGAG CTGAGCTTCC TCCAGGACAA GCTCAAGAGG 
CTAGAGAAGG AGCGGGCCAT GGCCGAGGGC AAGATCACCG TCAAGGAGGT GCTCAAGGTG 
GAGAAGGACG CGGCCACCGA GAGGGAGGTC AGCGATCTCA CCCGCCAATA TGAGGACGAG 
GCTGCCAAGG CTCGCGCTAG CCAGAGGGAG AAGACGGAGC TGCTCCGAAA GATATGGGCC 
TTGGAGGAGG AGAACGCCAA AGTGGTGGTG CAGGAGAAGG TGCGGGAGAT CGTGCGGCCA 
GACCCCAAGG CGGAAAGTGA AGTGGCGAAC CTCCGCCTGG AGCTTGTGGA GCAGGAGCGA 
AAGTACCGGG GTGCCGAGGA GCAGCTCCGG AGCTACCAGA GTGAGCTGGA GGCCCTCAGG 
AGGCGAGGCC CCCAGGTGGA AGTCAAAGAG GTGACTAAGG AAGTCATTAA GTACAAGACT 
GACCCTGAGA TGGAGAAGGA GCTTCAGCGG CTCAGGGAGG AGATCGTGGA CAAGACCAGA 
CTGATCGAAA GGTGTGATTT AGAGATCTAC CAGCTGAAAA AGGAAATCCA GGCCCTGAAA 
GACACCAAAC CCCAGGTCCA GACCAAAGAG GTGGTCCAGG AGATCCTCCA ATTCCAAGAA 
GACCCTCAAA CCAAGGAGGA GGTGGCGTCT CTGAGGGCAA AGCTCTCAGA GGAGCAGAAG 
AAACAAGTGG ATCTGGAGAG GGAAAGAGCT TCCCAGGAAG AGCAGATCGC CCGGAAAGAG 
GAGGAGCTCT CGCGGGTGAA GGAAAGGGTG GTGCAGCAGG AGGTGGTCAG GTATGAGGAG 
GAGCCAGGCC TGCGGGCCGA GGCGAGCGCC TTTGCCGAGA GCATCGATGT GGAGCTGCGG 
CAGATTGACA AGCTGCGGGC AGAGCTGCGG CGGCTGCAGC GCCGGCGCAC CGAGCTTGAG 
CGGCAGCTGG AGGAGCTAGA GCGCGAGCGG CAGGCCCGCA GGGAGGCCGA GCGCGAGGTA 
CAGCGGTTGC AGCAGCGGCT GGCAGCGCTG GAGCAGGAAG AAGCTGAGGC CCGTGAGAAG 
GTAACCCATA CGCAGAAGGT GGTGCTGCAG CAGGACCCGC AGCAGGCGCG AGAGCATGCC 
CTGCTCCGAC TCCAGCTGGA AGAAGAGCAG CACCGGCGGC AGCTCCTGGA GGGGGAGCTC 
GAGACCCTCC GGAGGAAACT GGCTGCACTG GAGAAGGCGG AGGTCAAGGA GAAGGTGGTG 
CTCTCCGAGA GTGTCCAGGT GGAGAAGGGC GACACCGAGC AAGAGATCCA GAGGCTCAAG 
AGCAGCCTGG AGGAGGAGAG CCGCAGCAAG CGCGAGCTGG ACGTCGAGGT GAGCCGGCTG 
GAAGCCAGGC TTTCGGAGCT GGAATTCCAT AACTCCAAGT CATCCAAGGA ACTAGACTTT 
CTGAGGGAAG AGAACCACAA ATTACAGCTG GAGAGGCAAA ACCTGCAGCT GGAGACCCGA 
AGGCTCCAAT CGGAAATCAA CATGGCAGCG ACGGAAACAC GAGACCTGCG GAACATGACC 
GTGGCGGACT CTGGGACCAA CCATGACTCC AGACTGTGGT CCCTGGAGAG GGAACTGGAT 
GACCTCAAGA GGCTCTCCAA GGACAAAGAC CTCGAGATCG ACGAGCTGCA GAAGCGCCTG 
GGCTCCGTGG CCGTCAAGCG GGAGCAGCGG GAGAACCACC TGCGGCGCTC CATCGTAGTC 
ATCCACCCTG ACACAGGCCG CGAGCTGTCC CCGGAGGAAG CCCACCGTGC CGGGCTCATT 
GACTGGAACA TGTTCGTGAA ACTCAGAAGC CAGGAGTGCG ACTGGGAGGA GATCTCAGTG 
AAGGGTCCCA ATGGGGAGTC CTCAGTGATA CACGACAGGA AGTCTGGCAA GAAGTTCTCC 
ATCGAAGAGG CCCTGCAGAG TGGCAGGCTG ACCCCTGCTC AGTATGACCG CTATGTCAAC 
AAGGATATGT CCATCCAGGA GCTGGCGGTC TTGGTATCTG GGCAGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002696.3
CDS	91..5361
Translation	MNSLFRKRNKGKYSPTVQTRSISNKELSELIEQLQKNADQVEKNIVDTEAKMQSDLARLQEGRQPEHRDVTLQKVLDSEKLLYVLEADAAIAKHMKHPQGDMIAEDIRQLKERVTNLRGKHKQIYRLAVKEVDPQVNWAALVEEKLDKLNNQSFGTDLPLVDHQVEEHNIFHNEVKAIGPHLAKDGDKEQNSELRAKYQKLLAASQARQQHLSSLQDYMQRCTNELYWLDQQAKGRMQYDWSDRNLDYPSRRRQYENFINRNLEAKEERINKLHSEGDQLLAAEHPGRNSIEAHMEAVHADWKEYLNLLICEESHLKYMEDYHQFHEDVKDAQELLRKVDSDLNQKYGPDFKDRYQIELLLRELDDQEKVLDKYEDVVQGLQKRGQQVVPLKYRRETPLKPIPVEALCDFEGEQGLISRGYSYTLQKNNGESWELMDSAGNKLIAPAVCFVIPPTDPEALALADSLGSQYRSVRQKAAGSKRTLQQRYEVLKTENPGDASDLQGRQLLAGLDKVASDLDRQEKAITGILRPPLEQGRAVQDSAERAKDLKNITNELLRIEPEKTRSTAEGEAFIQALPGSGTTPLLRTRVEDTNRKYEHLLQLLDLAQEKVDVANRLEKSLQQSWELLATHENHLNQDDTVPESSRVLDSKGQELAAMACELQAQKSLLGEVEQNLQAAKQCSSTLASRFQEHCPDLERQEAEVHKLGQRFNNLRQQVERRAQSLQSAKAAYEHFHRGHDHVLQFLVSIPSYEPQETDSLSQMETKLKNQKNLLDEIASREQEVQKICANSQQYQQAVKDYELEAEKLRSLLDLENGRSSHVSKRARLQSPATKVKEEEAALAAKFTEVYAINRQRLQNLEFALNLLRQQPEVEVTHETLQRNRPDSGVEEAWKIRKELDEETERRRQLENEVKSTQEEIWTLRNQGPQESVVRKEVLKKVPDPVLEESFQQLQRTLAEEQHKNQLLQEELEALQLQLRALEQETRDGGQEYVVKEVLRIEPDRAQADEVLQLREELEALRRQKGAREAEVLLLQQRVAALAEEKSRAQEKVTEKEVVKLQNDPQLEAEYQQLQEDHQRQDQLREKQEEELSFLQDKLKRLEKERAMAEGKITVKEVLKVEKDAATEREVSDLTRQYEDEAAKARASQREKTELLRKIWALEEENAKVVVQEKVREIVRPDPKAESEVANLRLELVEQERKYRGAEEQLRSYQSELEALRRRGPQVEVKEVTKEVIKYKTDPEMEKELQRLREEIVDKTRLIERCDLEIYQLKKEIQALKDTKPQVQTKEVVQEILQFQEDPQTKEEVASLRAKLSEEQKKQVDLERERASQEEQIARKEEELSRVKERVVQQEVVRYEEEPGLRAEASAFAESIDVELRQIDKLRAELRRLQRRRTELERQLEELERERQARREAEREVQRLQQRLAALEQEEAEAREKVTHTQKVVLQQDPQQAREHALLRLQLEEEQHRRQLLEGELETLRRKLAALEKAEVKEKVVLSESVQVEKGDTEQEIQRLKSSLEEESRSKRELDVEVSRLEARLSELEFHNSKSSKELDFLREENHKLQLERQNLQLETRRLQSEINMAATETRDLRNMTVADSGTNHDSRLWSLERELDDLKRLSKDKDLEIDELQKRLGSVAVKREQRENHLRRSIVVIHPDTGRELSPEEAHRAGLIDWNMFVKLRSQECDWEEISVKGPNGESSVIHDRKSGKKFSIEEALQSGRLTPAQYDRYVNKDMSIQELAVLVSGQK

Target ORF information:

RefSeq Version	NM_002705.4
Organism	Homo sapiens(human)
Definition	Homo sapiens periplakin (PPL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002705.4

ORF Insert Sequence:

ATGAACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCCACTGT GCAGACCCGG 
AGCATCTCTA ACAAGGAGCT CTCGGAGCTG ATCGAGCAGC TGCAGAAGAA TGCCGACCAG 
GTGGAGAAGA ACATCGTGGA CACAGAGGCC AAGATGCAGA GTGACCTGGC TCGGCTGCAG 
GAGGGTCGGC AGCCTGAGCA CCGGGACGTG ACCCTGCAGA AGGTGTTGGA CTCTGAGAAG 
CTGCTCTATG TGCTAGAGGC GGATGCGGCC ATTGCCAAGC ACATGAAGCA CCCACAGGGG 
GACATGATCG CCGAGGATAT CCGCCAGCTG AAGGAGCGTG TGACCAACCT GCGCGGGAAA 
CACAAGCAGA TCTACAGGCT GGCGGTGAAG GAAGTGGATC CACAGGTCAA CTGGGCGGCA 
CTGGTGGAGG AGAAGCTGGA CAAGCTGAAC AACCAGAGCT TTGGGACTGA CCTGCCGCTG 
GTGGACCACC AAGTGGAGGA GCATAACATC TTCCACAATG AGGTCAAGGC CATCGGGCCC 
CACCTGGCCA AGGACGGGGA CAAGGAGCAG AACAGCGAAC TCCGGGCCAA GTACCAGAAA 
CTGCTGGCAG CATCACAGGC CCGGCAGCAG CACCTGAGTT CGCTGCAGGA CTACATGCAG 
CGCTGCACCA ATGAGCTGTA CTGGCTGGAC CAGCAGGCCA AGGGCCGCAT GCAGTACGAC 
TGGAGTGACC GCAACCTCGA CTACCCCAGC CGCCGGCGCC AGTATGAGAA TTTCATCAAC 
CGGAACCTGG AGGCCAAAGA GGAGAGAATC AACAAACTGC ACAGCGAGGG CGACCAGCTG 
CTGGCGGCCG AGCACCCCGG GAGGAACTCC ATTGAGGCGC ACATGGAGGC TGTGCACGCA 
GACTGGAAGG AGTACCTGAA CCTGCTCATC TGCGAGGAGA GCCACCTCAA GTACATGGAG 
GACTACCACC AGTTTCACGA AGACGTGAAG GACGCTCAGG AGCTGCTGCG CAAGGTGGAC 
TCGGACCTGA ACCAGAAGTA TGGCCCTGAC TTCAAGGACC GGTACCAGAT TGAGCTGCTG 
CTGCGGGAGC TGGATGACCA GGAGAAGGTG CTGGACAAGT ATGAGGACGT GGTGCAGGGG 
CTGCAGAAGC GAGGCCAGCA GGTGGTGCCC CTCAAGTACC GCCGGGAGAC TCCGCTCAAG 
CCCATCCCCG TGGAGGCACT CTGTGACTTT GAGGGGGAGC AGGGCCTGAT CTCGCGGGGC 
TACAGCTACA CCCTGCAGAA GAACAACGGG GAGAGCTGGG AGCTCATGGA CAGCGCTGGG 
AACAAGCTGA TTGCTCCGGC CGTGTGTTTT GTGATCCCCC CCACAGACCC TGAGGCCCTG 
GCTCTGGCTG ACAGCCTGGG CAGCCAGTAC CGGAGCGTGC GGCAGAAGGC AGCTGGGAGC 
AAACGCACGC TGCAGCAGCG GTATGAGGTG CTGAAGACCG AGAATCCCGG AGATGCCTCT 
GACCTACAGG GGCGGCAGCT GCTGGCTGGC TTGGACAAGG TGGCCAGCGA CCTGGACCGG 
CAGGAGAAGG CCATCACAGG GATCCTGCGG CCACCACTGG AGCAAGGCCG GGCTGTGCAG 
GACAGTGCCG AGCGGGCCAA GGACCTCAAG AACATCACCA ACGAGCTACT GCGGATTGAA 
CCTGAGAAGA CGCGGAGCAC GGCTGAGGGC GAAGCCTTCA TCCAGGCCCT CCCAGGCAGT 
GGCACCACAC CCCTGCTGAG GACCCGGGTG GAGGACACCA ACCGGAAATA CGAGCACCTC 
CTGCAGCTGC TGGACTTGGC CCAGGAGAAG GTTGATGTGG CCAACCGCCT GGAGAAGAGC 
CTGCAGCAGA GCTGGGAGTT GCTGGCCACA CACGAGAACC ATCTGAATCA GGATGACACA 
GTGCCTGAGA GCAGCCGTGT CCTGGACAGC AAGGGGCAGG AGCTGGCGGC CATGGCCTGT 
GAGTTACAGG CCCAGAAGTC CCTCCTGGGT GAGGTGGAGC AGAACTTGCA GGCGGCCAAG 
CAGTGCTCGA GCACACTGGC CAGCCGCTTC CAGGAGCACT GTCCGGACCT GGAGCGCCAG 
GAGGCCGAGG TGCACAAGCT GGGCCAGCGT TTCAACAACC TGCGCCAGCA GGTGGAACGC 
AGGGCGCAGA GCCTACAGAG CGCCAAGGCA GCCTACGAGC ACTTCCACCG CGGCCATGAC 
CACGTGCTGC AGTTCCTAGT CAGCATCCCC AGTTACGAGC CCCAGGAGAC AGACAGCCTC 
AGCCAGATGG AGACCAAGCT GAAGAACCAG AAGAACCTGC TAGATGAGAT AGCAAGTAGG 
GAGCAGGAAG TACAGAAGAT CTGTGCCAAT TCCCAGCAGT ACCAGCAAGC TGTAAAGGAC 
TATGAGTTAG AAGCAGAAAA ACTAAGGTCT CTTCTCGACT TGGAGAATGG AAGGAGCAGC 
CACGTGAGCA AGAGAGCCAG GCTCCAATCT CCTGCCACCA AAGTGAAGGA AGAGGAAGCA 
GCACTTGCCG CCAAGTTCAC TGAAGTTTAT GCCATCAACA GACAGAGGCT GCAGAATCTG 
GAGTTTGCTC TGAATCTCCT CAGACAGCAG CCGGAAGTAG AAGTGACCCA TGAGACCCTG 
CAAAGGAATA GGCCGGACTC TGGAGTGGAG GAGGCGTGGA AGATCAGGAA GGAACTGGAT 
GAGGAGACTG AGCGGAGGCG GCAGCTGGAG AACGAGGTCA AGAGCACCCA GGAAGAAATC 
TGGACCTTGA GGAATCAGGG GCCTCAGGAA TCGGTGGTGA GGAAGGAGGT GCTCAAGAAG 
GTGCCGGATC CCGTGCTGGA GGAGAGCTTC CAGCAGCTGC AGCGGACGCT GGCAGAGGAG 
CAGCACAAGA ACCAGCTGCT GCAGGAGGAG CTGGAGGCAC TGCAGCTGCA GCTGCGTGCC 
CTGGAGCAGG AGACCAGAGA CGGGGGGCAG GAGTACGTGG TCAAGGAGGT CCTGCGCATC 
GAGCCTGACA GGGCCCAGGC GGATGAGGTC TTGCAGCTGC GGGAGGAGCT GGAGGCACTG 
AGGCGGCAGA AGGGCGCCCG GGAGGCAGAG GTGCTCCTCC TGCAGCAGCG TGTGGCCGCC 
CTGGCTGAAG AGAAGAGCCG GGCGCAGGAG AAGGTCACAG AGAAAGAGGT GGTGAAACTG 
CAGAATGACC CCCAGCTGGA GGCAGAGTAC CAGCAGCTGC AGGAGGACCA CCAGCGCCAG 
GACCAGCTCA GGGAGAAGCA GGAGGAGGAG CTGAGCTTCC TCCAGGACAA GCTCAAGAGG 
CTAGAGAAGG AGCGGGCCAT GGCCGAGGGC AAGATCACCG TCAAGGAGGT GCTCAAGGTG 
GAGAAGGACG CGGCCACCGA GAGGGAGGTC AGCGATCTCA CCCGCCAATA TGAGGACGAG 
GCTGCCAAGG CTCGCGCTAG CCAGAGGGAG AAGACGGAGC TGCTCCGAAA GATATGGGCC 
TTGGAGGAGG AGAACGCCAA AGTGGTGGTG CAGGAGAAGG TGCGGGAGAT CGTGCGGCCA 
GACCCCAAGG CGGAAAGTGA AGTGGCGAAC CTCCGCCTGG AGCTTGTGGA GCAGGAGCGA 
AAGTACCGGG GTGCCGAGGA GCAGCTCCGG AGCTACCAGA GTGAGCTGGA GGCCCTCAGG 
AGGCGAGGCC CCCAGGTGGA AGTCAAAGAG GTGACTAAGG AAGTCATTAA GTACAAGACT 
GACCCTGAGA TGGAGAAGGA GCTTCAGCGG CTCAGGGAGG AGATCGTGGA CAAGACCAGA 
CTGATCGAAA GGTGTGATTT AGAGATCTAC CAGCTGAAAA AGGAAATCCA GGCCCTGAAA 
GACACCAAAC CCCAGGTCCA GACCAAAGAG GTGGTCCAGG AGATCCTCCA ATTCCAAGAA 
GACCCTCAAA CCAAGGAGGA GGTGGCGTCT CTGAGGGCAA AGCTCTCAGA GGAGCAGAAG 
AAACAAGTGG ATCTGGAGAG GGAAAGAGCT TCCCAGGAAG AGCAGATCGC CCGGAAAGAG 
GAGGAGCTCT CGCGGGTGAA GGAAAGGGTG GTGCAGCAGG AGGTGGTCAG GTATGAGGAG 
GAGCCAGGCC TGCGGGCCGA GGCGAGCGCC TTTGCCGAGA GCATCGATGT GGAGCTGCGG 
CAGATTGACA AGCTGCGGGC AGAGCTGCGG CGGCTGCAGC GCCGGCGCAC CGAGCTTGAG 
CGGCAGCTGG AGGAGCTAGA GCGCGAGCGG CAGGCCCGCA GGGAGGCCGA GCGCGAGGTA 
CAGCGGTTGC AGCAGCGGCT GGCAGCGCTG GAGCAGGAAG AAGCTGAGGC CCGTGAGAAG 
GTAACCCATA CGCAGAAGGT GGTGCTGCAG CAGGACCCGC AGCAGGCGCG AGAGCATGCC 
CTGCTCCGAC TCCAGCTGGA AGAAGAGCAG CACCGGCGGC AGCTCCTGGA GGGGGAGCTC 
GAGACCCTCC GGAGGAAACT GGCTGCACTG GAGAAGGCGG AGGTCAAGGA GAAGGTGGTG 
CTCTCCGAGA GTGTCCAGGT GGAGAAGGGC GACACCGAGC AAGAGATCCA GAGGCTCAAG 
AGCAGCCTGG AGGAGGAGAG CCGCAGCAAG CGCGAGCTGG ACGTCGAGGT GAGCCGGCTG 
GAAGCCAGGC TTTCGGAGCT GGAATTCCAT AACTCCAAGT CATCCAAGGA ACTAGACTTT 
CTGAGGGAAG AGAACCACAA ATTACAGCTG GAGAGGCAAA ACCTGCAGCT GGAGACCCGA 
AGGCTCCAAT CGGAAATCAA CATGGCAGCG ACGGAAACAC GAGACCTGCG GAACATGACC 
GTGGCGGACT CTGGGACCAA CCATGACTCC AGACTGTGGT CCCTGGAGAG GGAACTGGAT 
GACCTCAAGA GGCTCTCCAA GGACAAAGAC CTCGAGATCG ACGAGCTGCA GAAGCGCCTG 
GGCTCCGTGG CCGTCAAGCG GGAGCAGCGG GAGAACCACC TGCGGCGCTC CATCGTAGTC 
ATCCACCCTG ACACAGGCCG CGAGCTGTCC CCGGAGGAAG CCCACCGTGC CGGGCTCATT 
GACTGGAACA TGTTCGTGAA ACTCAGAAGC CAGGAGTGCG ACTGGGAGGA GATCTCAGTG 
AAGGGTCCCA ATGGGGAGTC CTCAGTGATA CACGACAGGA AGTCTGGCAA GAAGTTCTCC 
ATCGAAGAGG CCCTGCAGAG TGGCAGGCTG ACCCCTGCTC AGTATGACCG CTATGTCAAC 
AAGGATATGT CCATCCAGGA GCTGGCGGTC TTGGTATCTG GGCAGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPL ( NM_002705.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002696.4 Homo sapiens periplakin (PPL), mRNA.

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CloneID	OHu107375
Clone ID Related Accession (Same CDS sequence)	NM_002705.5
Accession Version	NM_002705.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	periplakin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC027687.7. On Nov 24, 2018 this sequence version replaced NM_002705.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF001691.1, AF013717.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000345988.7/ ENSP00000340510.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCCACTGT GCAGACCCGG 
AGCATCTCTA ACAAGGAGCT CTCGGAGCTG ATCGAGCAGC TGCAGAAGAA TGCCGACCAG 
GTGGAGAAGA ACATCGTGGA CACAGAGGCC AAGATGCAGA GTGACCTGGC TCGGCTGCAG 
GAGGGTCGGC AGCCTGAGCA CCGGGACGTG ACCCTGCAGA AGGTGTTGGA CTCTGAGAAG 
CTGCTCTATG TGCTAGAGGC GGATGCGGCC ATTGCCAAGC ACATGAAGCA CCCACAGGGG 
GACATGATCG CCGAGGATAT CCGCCAGCTG AAGGAGCGTG TGACCAACCT GCGCGGGAAA 
CACAAGCAGA TCTACAGGCT GGCGGTGAAG GAAGTGGATC CACAGGTCAA CTGGGCGGCA 
CTGGTGGAGG AGAAGCTGGA CAAGCTGAAC AACCAGAGCT TTGGGACTGA CCTGCCGCTG 
GTGGACCACC AAGTGGAGGA GCATAACATC TTCCACAATG AGGTCAAGGC CATCGGGCCC 
CACCTGGCCA AGGACGGGGA CAAGGAGCAG AACAGCGAAC TCCGGGCCAA GTACCAGAAA 
CTGCTGGCAG CATCACAGGC CCGGCAGCAG CACCTGAGTT CGCTGCAGGA CTACATGCAG 
CGCTGCACCA ATGAGCTGTA CTGGCTGGAC CAGCAGGCCA AGGGCCGCAT GCAGTACGAC 
TGGAGTGACC GCAACCTCGA CTACCCCAGC CGCCGGCGCC AGTATGAGAA TTTCATCAAC 
CGGAACCTGG AGGCCAAAGA GGAGAGAATC AACAAACTGC ACAGCGAGGG CGACCAGCTG 
CTGGCGGCCG AGCACCCCGG GAGGAACTCC ATTGAGGCGC ACATGGAGGC TGTGCACGCA 
GACTGGAAGG AGTACCTGAA CCTGCTCATC TGCGAGGAGA GCCACCTCAA GTACATGGAG 
GACTACCACC AGTTTCACGA AGACGTGAAG GACGCTCAGG AGCTGCTGCG CAAGGTGGAC 
TCGGACCTGA ACCAGAAGTA TGGCCCTGAC TTCAAGGACC GGTACCAGAT TGAGCTGCTG 
CTGCGGGAGC TGGATGACCA GGAGAAGGTG CTGGACAAGT ATGAGGACGT GGTGCAGGGG 
CTGCAGAAGC GAGGCCAGCA GGTGGTGCCC CTCAAGTACC GCCGGGAGAC TCCGCTCAAG 
CCCATCCCCG TGGAGGCACT CTGTGACTTT GAGGGGGAGC AGGGCCTGAT CTCGCGGGGC 
TACAGCTACA CCCTGCAGAA GAACAACGGG GAGAGCTGGG AGCTCATGGA CAGCGCTGGG 
AACAAGCTGA TTGCTCCGGC CGTGTGTTTT GTGATCCCCC CCACAGACCC TGAGGCCCTG 
GCTCTGGCTG ACAGCCTGGG CAGCCAGTAC CGGAGCGTGC GGCAGAAGGC AGCTGGGAGC 
AAACGCACGC TGCAGCAGCG GTATGAGGTG CTGAAGACCG AGAATCCCGG AGATGCCTCT 
GACCTACAGG GGCGGCAGCT GCTGGCTGGC TTGGACAAGG TGGCCAGCGA CCTGGACCGG 
CAGGAGAAGG CCATCACAGG GATCCTGCGG CCACCACTGG AGCAAGGCCG GGCTGTGCAG 
GACAGTGCCG AGCGGGCCAA GGACCTCAAG AACATCACCA ACGAGCTACT GCGGATTGAA 
CCTGAGAAGA CGCGGAGCAC GGCTGAGGGC GAAGCCTTCA TCCAGGCCCT CCCAGGCAGT 
GGCACCACAC CCCTGCTGAG GACCCGGGTG GAGGACACCA ACCGGAAATA CGAGCACCTC 
CTGCAGCTGC TGGACTTGGC CCAGGAGAAG GTTGATGTGG CCAACCGCCT GGAGAAGAGC 
CTGCAGCAGA GCTGGGAGTT GCTGGCCACA CACGAGAACC ATCTGAATCA GGATGACACA 
GTGCCTGAGA GCAGCCGTGT CCTGGACAGC AAGGGGCAGG AGCTGGCGGC CATGGCCTGT 
GAGTTACAGG CCCAGAAGTC CCTCCTGGGT GAGGTGGAGC AGAACTTGCA GGCGGCCAAG 
CAGTGCTCGA GCACACTGGC CAGCCGCTTC CAGGAGCACT GTCCGGACCT GGAGCGCCAG 
GAGGCCGAGG TGCACAAGCT GGGCCAGCGT TTCAACAACC TGCGCCAGCA GGTGGAACGC 
AGGGCGCAGA GCCTACAGAG CGCCAAGGCA GCCTACGAGC ACTTCCACCG CGGCCATGAC 
CACGTGCTGC AGTTCCTAGT CAGCATCCCC AGTTACGAGC CCCAGGAGAC AGACAGCCTC 
AGCCAGATGG AGACCAAGCT GAAGAACCAG AAGAACCTGC TAGATGAGAT AGCAAGTAGG 
GAGCAGGAAG TACAGAAGAT CTGTGCCAAT TCCCAGCAGT ACCAGCAAGC TGTAAAGGAC 
TATGAGTTAG AAGCAGAAAA ACTAAGGTCT CTTCTCGACT TGGAGAATGG AAGGAGAAGC 
CACGTGAGCA AGAGAGCCAG GCTCCAATCT CCTGCCACCA AAGTGAAGGA AGAGGAAGCA 
GCACTTGCCG CCAAGTTCAC TGAAGTTTAT GCCATCAACA GACAGAGGCT GCAGAATCTG 
GAGTTTGCTC TGAATCTCCT CAGACAGCAG CCGGAAGTAG AAGTGACCCA TGAGACCCTG 
CAAAGGAATA GGCCGGACTC TGGAGTGGAG GAGGCGTGGA AGATCAGGAA GGAACTGGAT 
GAGGAGACTG AGCGGAGGCG GCAGCTGGAG AACGAGGTCA AGAGCACCCA GGAAGAAATC 
TGGACCTTGA GGAATCAGGG GCCTCAGGAA TCGGTGGTGA GGAAGGAGGT GCTCAAGAAG 
GTGCCGGATC CCGTGCTGGA GGAGAGCTTC CAGCAGCTGC AGCGGACGCT GGCAGAGGAG 
CAGCACAAGA ACCAGCTGCT GCAGGAGGAG CTGGAGGCAC TGCAGCTGCA GCTGCGTGCC 
CTGGAGCAGG AGACCAGAGA CGGGGGGCAG GAGTACGTGG TCAAGGAGGT CCTGCGCATC 
GAGCCTGACA GGGCCCAGGC GGATGAGGTC TTGCAGCTGC GGGAGGAGCT GGAGGCACTG 
AGGCGGCAGA AGGGCGCCCG GGAGGCAGAG GTGCTCCTCC TGCAGCAGCG TGTGGCCGCC 
CTGGCTGAAG AGAAGAGCCG GGCGCAGGAG AAGGTCACAG AGAAAGAGGT GGTGAAACTG 
CAGAATGACC CCCAGCTGGA GGCAGAGTAC CAGCAGCTGC AGGAGGACCA CCAGCGCCAG 
GACCAGCTCA GGGAGAAGCA GGAGGAGGAG CTGAGCTTCC TCCAGGACAA GCTCAAGAGG 
CTAGAGAAGG AGCGGGCCAT GGCCGAGGGC AAGATCACCG TCAAGGAGGT GCTCAAGGTG 
GAGAAGGACG CGGCCACCGA GAGGGAGGTC AGCGATCTCA CCCGCCAATA TGAGGACGAG 
GCTGCCAAGG CTCGCGCTAG CCAGAGGGAG AAGACGGAGC TGCTCCGAAA GATATGGGCC 
TTGGAGGAGG AGAACGCCAA AGTGGTGGTG CAGGAGAAGG TGCGGGAGAT CGTGCGGCCA 
GACCCCAAGG CGGAAAGTGA AGTGGCGAAC CTCCGCCTGG AGCTTGTGGA GCAGGAGCGA 
AAGTACCGGG GTGCCGAGGA GCAGCTCCGG AGCTACCAGA GTGAGCTGGA GGCCCTCAGG 
AGGCGAGGCC CCCAGGTGGA AGTCAAAGAG GTGACTAAGG AAGTCATTAA GTACAAGACT 
GACCCTGAGA TGGAGAAGGA GCTTCAGCGG CTCAGGGAGG AGATCGTGGA CAAGACCAGA 
CTGATCGAAA GGTGTGATTT AGAGATCTAC CAGCTGAAAA AGGAAATCCA GGCCCTGAAA 
GACACCAAAC CCCAGGTCCA GACCAAAGAG GTGGTCCAGG AGATCCTCCA ATTCCAAGAA 
GACCCTCAAA CCAAGGAGGA GGTGGCGTCT CTGAGGGCAA AGCTCTCAGA GGAGCAGAAG 
AAACAAGTGG ATCTGGAGAG GGAAAGAGCT TCCCAGGAAG AGCAGATCGC CCGGAAAGAG 
GAGGAGCTCT CGCGGGTGAA GGAAAGGGTG GTGCAGCAGG AGGTGGTCAG GTATGAGGAG 
GAGCCAGGCC TGCGGGCCGA GGCGAGCGCC TTTGCCGAGA GCATCGATGT GGAGCTGCGG 
CAGATTGACA AGCTGCGGGC AGAGCTGCGG CGGCTGCAGC GCCGGCGCAC CGAGCTTGAG 
CGGCAGCTGG AGGAGCTAGA GCGCGAGCGG CAGGCCCGCA GGGAGGCCGA GCGCGAGGTA 
CAGCGGTTGC AGCAGCGGCT GGCAGCGCTG GAGCAGGAAG AAGCTGAGGC CCGTGAGAAG 
GTAACCCATA CGCAGAAGGT GGTGCTGCAG CAGGACCCGC AGCAGGCGCG AGAGCATGCC 
CTGCTCCGAC TCCAGCTGGA AGAAGAGCAG CACCGGCGGC AGCTCCTGGA GGGGGAGCTC 
GAGACCCTCC GGAGGAAACT GGCTGCACTG GAGAAGGCGG AGGTCAAGGA GAAGGTGGTG 
CTCTCCGAGA GTGTCCAGGT GGAGAAGGGC GACACCGAGC AAGAGATCCA GAGGCTCAAG 
AGCAGCCTGG AGGAGGAGAG CCGCAGCAAG CGCGAGCTGG ACGTCGAGGT GAGCCGGCTG 
GAAGCCAGGC TTTCGGAGCT GGAATTCCAT AACTCCAAGT CATCCAAGGA ACTAGACTTT 
CTGAGGGAAG AGAACCACAA ATTACAGCTG GAGAGGCAAA ACCTGCAGCT GGAGACCCGA 
AGGCTCCAAT CGGAAATCAA CATGGCAGCG ACGGAAACAC GAGACCTGCG GAACATGACC 
GTGGCGGACT CTGGGACCAA CCATGACTCC AGACTGTGGT CCCTGGAGAG GGAACTGGAT 
GACCTCAAGA GGCTCTCCAA GGACAAAGAC CTCGAGATCG ACGAGCTGCA GAAGCGCCTG 
GGCTCCGTGG CCGTCAAGCG GGAGCAGCGG GAGAACCACC TGCGGCGCTC CATCGTAGTC 
ATCCACCCTG ACACAGGCCG CGAGCTGTCC CCGGAGGAAG CCCACCGTGC CGGGCTCATT 
GACTGGAACA TGTTCGTGAA ACTCAGAAGC CAGGAGTGCG ACTGGGAGGA GATCTCAGTG 
AAGGGTCCCA ATGGGGAGTC CTCAGTGATA CACGACAGGA AGTCTGGCAA GAAGTTCTCC 
ATCGAAGAGG CCCTGCAGAG TGGCAGGCTG ACCCCTGCTC AGTATGACCG CTATGTCAAC 
AAGGATATGT CCATCCAGGA GCTGGCGGTC TTGGTATCTG GGCAGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002696.4
CDS	104..5374
Translation	MNSLFRKRNKGKYSPTVQTRSISNKELSELIEQLQKNADQVEKNIVDTEAKMQSDLARLQEGRQPEHRDVTLQKVLDSEKLLYVLEADAAIAKHMKHPQGDMIAEDIRQLKERVTNLRGKHKQIYRLAVKEVDPQVNWAALVEEKLDKLNNQSFGTDLPLVDHQVEEHNIFHNEVKAIGPHLAKDGDKEQNSELRAKYQKLLAASQARQQHLSSLQDYMQRCTNELYWLDQQAKGRMQYDWSDRNLDYPSRRRQYENFINRNLEAKEERINKLHSEGDQLLAAEHPGRNSIEAHMEAVHADWKEYLNLLICEESHLKYMEDYHQFHEDVKDAQELLRKVDSDLNQKYGPDFKDRYQIELLLRELDDQEKVLDKYEDVVQGLQKRGQQVVPLKYRRETPLKPIPVEALCDFEGEQGLISRGYSYTLQKNNGESWELMDSAGNKLIAPAVCFVIPPTDPEALALADSLGSQYRSVRQKAAGSKRTLQQRYEVLKTENPGDASDLQGRQLLAGLDKVASDLDRQEKAITGILRPPLEQGRAVQDSAERAKDLKNITNELLRIEPEKTRSTAEGEAFIQALPGSGTTPLLRTRVEDTNRKYEHLLQLLDLAQEKVDVANRLEKSLQQSWELLATHENHLNQDDTVPESSRVLDSKGQELAAMACELQAQKSLLGEVEQNLQAAKQCSSTLASRFQEHCPDLERQEAEVHKLGQRFNNLRQQVERRAQSLQSAKAAYEHFHRGHDHVLQFLVSIPSYEPQETDSLSQMETKLKNQKNLLDEIASREQEVQKICANSQQYQQAVKDYELEAEKLRSLLDLENGRRSHVSKRARLQSPATKVKEEEAALAAKFTEVYAINRQRLQNLEFALNLLRQQPEVEVTHETLQRNRPDSGVEEAWKIRKELDEETERRRQLENEVKSTQEEIWTLRNQGPQESVVRKEVLKKVPDPVLEESFQQLQRTLAEEQHKNQLLQEELEALQLQLRALEQETRDGGQEYVVKEVLRIEPDRAQADEVLQLREELEALRRQKGAREAEVLLLQQRVAALAEEKSRAQEKVTEKEVVKLQNDPQLEAEYQQLQEDHQRQDQLREKQEEELSFLQDKLKRLEKERAMAEGKITVKEVLKVEKDAATEREVSDLTRQYEDEAAKARASQREKTELLRKIWALEEENAKVVVQEKVREIVRPDPKAESEVANLRLELVEQERKYRGAEEQLRSYQSELEALRRRGPQVEVKEVTKEVIKYKTDPEMEKELQRLREEIVDKTRLIERCDLEIYQLKKEIQALKDTKPQVQTKEVVQEILQFQEDPQTKEEVASLRAKLSEEQKKQVDLERERASQEEQIARKEEELSRVKERVVQQEVVRYEEEPGLRAEASAFAESIDVELRQIDKLRAELRRLQRRRTELERQLEELERERQARREAEREVQRLQQRLAALEQEEAEAREKVTHTQKVVLQQDPQQAREHALLRLQLEEEQHRRQLLEGELETLRRKLAALEKAEVKEKVVLSESVQVEKGDTEQEIQRLKSSLEEESRSKRELDVEVSRLEARLSELEFHNSKSSKELDFLREENHKLQLERQNLQLETRRLQSEINMAATETRDLRNMTVADSGTNHDSRLWSLERELDDLKRLSKDKDLEIDELQKRLGSVAVKREQRENHLRRSIVVIHPDTGRELSPEEAHRAGLIDWNMFVKLRSQECDWEEISVKGPNGESSVIHDRKSGKKFSIEEALQSGRLTPAQYDRYVNKDMSIQELAVLVSGQK

Target ORF information:

RefSeq Version	NM_002705.5
Organism	Homo sapiens(human)
Definition	Homo sapiens periplakin (PPL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002705.5

ORF Insert Sequence:

ATGAACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCCACTGT GCAGACCCGG 
AGCATCTCTA ACAAGGAGCT CTCGGAGCTG ATCGAGCAGC TGCAGAAGAA TGCCGACCAG 
GTGGAGAAGA ACATCGTGGA CACAGAGGCC AAGATGCAGA GTGACCTGGC TCGGCTGCAG 
GAGGGTCGGC AGCCTGAGCA CCGGGACGTG ACCCTGCAGA AGGTGTTGGA CTCTGAGAAG 
CTGCTCTATG TGCTAGAGGC GGATGCGGCC ATTGCCAAGC ACATGAAGCA CCCACAGGGG 
GACATGATCG CCGAGGATAT CCGCCAGCTG AAGGAGCGTG TGACCAACCT GCGCGGGAAA 
CACAAGCAGA TCTACAGGCT GGCGGTGAAG GAAGTGGATC CACAGGTCAA CTGGGCGGCA 
CTGGTGGAGG AGAAGCTGGA CAAGCTGAAC AACCAGAGCT TTGGGACTGA CCTGCCGCTG 
GTGGACCACC AAGTGGAGGA GCATAACATC TTCCACAATG AGGTCAAGGC CATCGGGCCC 
CACCTGGCCA AGGACGGGGA CAAGGAGCAG AACAGCGAAC TCCGGGCCAA GTACCAGAAA 
CTGCTGGCAG CATCACAGGC CCGGCAGCAG CACCTGAGTT CGCTGCAGGA CTACATGCAG 
CGCTGCACCA ATGAGCTGTA CTGGCTGGAC CAGCAGGCCA AGGGCCGCAT GCAGTACGAC 
TGGAGTGACC GCAACCTCGA CTACCCCAGC CGCCGGCGCC AGTATGAGAA TTTCATCAAC 
CGGAACCTGG AGGCCAAAGA GGAGAGAATC AACAAACTGC ACAGCGAGGG CGACCAGCTG 
CTGGCGGCCG AGCACCCCGG GAGGAACTCC ATTGAGGCGC ACATGGAGGC TGTGCACGCA 
GACTGGAAGG AGTACCTGAA CCTGCTCATC TGCGAGGAGA GCCACCTCAA GTACATGGAG 
GACTACCACC AGTTTCACGA AGACGTGAAG GACGCTCAGG AGCTGCTGCG CAAGGTGGAC 
TCGGACCTGA ACCAGAAGTA TGGCCCTGAC TTCAAGGACC GGTACCAGAT TGAGCTGCTG 
CTGCGGGAGC TGGATGACCA GGAGAAGGTG CTGGACAAGT ATGAGGACGT GGTGCAGGGG 
CTGCAGAAGC GAGGCCAGCA GGTGGTGCCC CTCAAGTACC GCCGGGAGAC TCCGCTCAAG 
CCCATCCCCG TGGAGGCACT CTGTGACTTT GAGGGGGAGC AGGGCCTGAT CTCGCGGGGC 
TACAGCTACA CCCTGCAGAA GAACAACGGG GAGAGCTGGG AGCTCATGGA CAGCGCTGGG 
AACAAGCTGA TTGCTCCGGC CGTGTGTTTT GTGATCCCCC CCACAGACCC TGAGGCCCTG 
GCTCTGGCTG ACAGCCTGGG CAGCCAGTAC CGGAGCGTGC GGCAGAAGGC AGCTGGGAGC 
AAACGCACGC TGCAGCAGCG GTATGAGGTG CTGAAGACCG AGAATCCCGG AGATGCCTCT 
GACCTACAGG GGCGGCAGCT GCTGGCTGGC TTGGACAAGG TGGCCAGCGA CCTGGACCGG 
CAGGAGAAGG CCATCACAGG GATCCTGCGG CCACCACTGG AGCAAGGCCG GGCTGTGCAG 
GACAGTGCCG AGCGGGCCAA GGACCTCAAG AACATCACCA ACGAGCTACT GCGGATTGAA 
CCTGAGAAGA CGCGGAGCAC GGCTGAGGGC GAAGCCTTCA TCCAGGCCCT CCCAGGCAGT 
GGCACCACAC CCCTGCTGAG GACCCGGGTG GAGGACACCA ACCGGAAATA CGAGCACCTC 
CTGCAGCTGC TGGACTTGGC CCAGGAGAAG GTTGATGTGG CCAACCGCCT GGAGAAGAGC 
CTGCAGCAGA GCTGGGAGTT GCTGGCCACA CACGAGAACC ATCTGAATCA GGATGACACA 
GTGCCTGAGA GCAGCCGTGT CCTGGACAGC AAGGGGCAGG AGCTGGCGGC CATGGCCTGT 
GAGTTACAGG CCCAGAAGTC CCTCCTGGGT GAGGTGGAGC AGAACTTGCA GGCGGCCAAG 
CAGTGCTCGA GCACACTGGC CAGCCGCTTC CAGGAGCACT GTCCGGACCT GGAGCGCCAG 
GAGGCCGAGG TGCACAAGCT GGGCCAGCGT TTCAACAACC TGCGCCAGCA GGTGGAACGC 
AGGGCGCAGA GCCTACAGAG CGCCAAGGCA GCCTACGAGC ACTTCCACCG CGGCCATGAC 
CACGTGCTGC AGTTCCTAGT CAGCATCCCC AGTTACGAGC CCCAGGAGAC AGACAGCCTC 
AGCCAGATGG AGACCAAGCT GAAGAACCAG AAGAACCTGC TAGATGAGAT AGCAAGTAGG 
GAGCAGGAAG TACAGAAGAT CTGTGCCAAT TCCCAGCAGT ACCAGCAAGC TGTAAAGGAC 
TATGAGTTAG AAGCAGAAAA ACTAAGGTCT CTTCTCGACT TGGAGAATGG AAGGAGAAGC 
CACGTGAGCA AGAGAGCCAG GCTCCAATCT CCTGCCACCA AAGTGAAGGA AGAGGAAGCA 
GCACTTGCCG CCAAGTTCAC TGAAGTTTAT GCCATCAACA GACAGAGGCT GCAGAATCTG 
GAGTTTGCTC TGAATCTCCT CAGACAGCAG CCGGAAGTAG AAGTGACCCA TGAGACCCTG 
CAAAGGAATA GGCCGGACTC TGGAGTGGAG GAGGCGTGGA AGATCAGGAA GGAACTGGAT 
GAGGAGACTG AGCGGAGGCG GCAGCTGGAG AACGAGGTCA AGAGCACCCA GGAAGAAATC 
TGGACCTTGA GGAATCAGGG GCCTCAGGAA TCGGTGGTGA GGAAGGAGGT GCTCAAGAAG 
GTGCCGGATC CCGTGCTGGA GGAGAGCTTC CAGCAGCTGC AGCGGACGCT GGCAGAGGAG 
CAGCACAAGA ACCAGCTGCT GCAGGAGGAG CTGGAGGCAC TGCAGCTGCA GCTGCGTGCC 
CTGGAGCAGG AGACCAGAGA CGGGGGGCAG GAGTACGTGG TCAAGGAGGT CCTGCGCATC 
GAGCCTGACA GGGCCCAGGC GGATGAGGTC TTGCAGCTGC GGGAGGAGCT GGAGGCACTG 
AGGCGGCAGA AGGGCGCCCG GGAGGCAGAG GTGCTCCTCC TGCAGCAGCG TGTGGCCGCC 
CTGGCTGAAG AGAAGAGCCG GGCGCAGGAG AAGGTCACAG AGAAAGAGGT GGTGAAACTG 
CAGAATGACC CCCAGCTGGA GGCAGAGTAC CAGCAGCTGC AGGAGGACCA CCAGCGCCAG 
GACCAGCTCA GGGAGAAGCA GGAGGAGGAG CTGAGCTTCC TCCAGGACAA GCTCAAGAGG 
CTAGAGAAGG AGCGGGCCAT GGCCGAGGGC AAGATCACCG TCAAGGAGGT GCTCAAGGTG 
GAGAAGGACG CGGCCACCGA GAGGGAGGTC AGCGATCTCA CCCGCCAATA TGAGGACGAG 
GCTGCCAAGG CTCGCGCTAG CCAGAGGGAG AAGACGGAGC TGCTCCGAAA GATATGGGCC 
TTGGAGGAGG AGAACGCCAA AGTGGTGGTG CAGGAGAAGG TGCGGGAGAT CGTGCGGCCA 
GACCCCAAGG CGGAAAGTGA AGTGGCGAAC CTCCGCCTGG AGCTTGTGGA GCAGGAGCGA 
AAGTACCGGG GTGCCGAGGA GCAGCTCCGG AGCTACCAGA GTGAGCTGGA GGCCCTCAGG 
AGGCGAGGCC CCCAGGTGGA AGTCAAAGAG GTGACTAAGG AAGTCATTAA GTACAAGACT 
GACCCTGAGA TGGAGAAGGA GCTTCAGCGG CTCAGGGAGG AGATCGTGGA CAAGACCAGA 
CTGATCGAAA GGTGTGATTT AGAGATCTAC CAGCTGAAAA AGGAAATCCA GGCCCTGAAA 
GACACCAAAC CCCAGGTCCA GACCAAAGAG GTGGTCCAGG AGATCCTCCA ATTCCAAGAA 
GACCCTCAAA CCAAGGAGGA GGTGGCGTCT CTGAGGGCAA AGCTCTCAGA GGAGCAGAAG 
AAACAAGTGG ATCTGGAGAG GGAAAGAGCT TCCCAGGAAG AGCAGATCGC CCGGAAAGAG 
GAGGAGCTCT CGCGGGTGAA GGAAAGGGTG GTGCAGCAGG AGGTGGTCAG GTATGAGGAG 
GAGCCAGGCC TGCGGGCCGA GGCGAGCGCC TTTGCCGAGA GCATCGATGT GGAGCTGCGG 
CAGATTGACA AGCTGCGGGC AGAGCTGCGG CGGCTGCAGC GCCGGCGCAC CGAGCTTGAG 
CGGCAGCTGG AGGAGCTAGA GCGCGAGCGG CAGGCCCGCA GGGAGGCCGA GCGCGAGGTA 
CAGCGGTTGC AGCAGCGGCT GGCAGCGCTG GAGCAGGAAG AAGCTGAGGC CCGTGAGAAG 
GTAACCCATA CGCAGAAGGT GGTGCTGCAG CAGGACCCGC AGCAGGCGCG AGAGCATGCC 
CTGCTCCGAC TCCAGCTGGA AGAAGAGCAG CACCGGCGGC AGCTCCTGGA GGGGGAGCTC 
GAGACCCTCC GGAGGAAACT GGCTGCACTG GAGAAGGCGG AGGTCAAGGA GAAGGTGGTG 
CTCTCCGAGA GTGTCCAGGT GGAGAAGGGC GACACCGAGC AAGAGATCCA GAGGCTCAAG 
AGCAGCCTGG AGGAGGAGAG CCGCAGCAAG CGCGAGCTGG ACGTCGAGGT GAGCCGGCTG 
GAAGCCAGGC TTTCGGAGCT GGAATTCCAT AACTCCAAGT CATCCAAGGA ACTAGACTTT 
CTGAGGGAAG AGAACCACAA ATTACAGCTG GAGAGGCAAA ACCTGCAGCT GGAGACCCGA 
AGGCTCCAAT CGGAAATCAA CATGGCAGCG ACGGAAACAC GAGACCTGCG GAACATGACC 
GTGGCGGACT CTGGGACCAA CCATGACTCC AGACTGTGGT CCCTGGAGAG GGAACTGGAT 
GACCTCAAGA GGCTCTCCAA GGACAAAGAC CTCGAGATCG ACGAGCTGCA GAAGCGCCTG 
GGCTCCGTGG CCGTCAAGCG GGAGCAGCGG GAGAACCACC TGCGGCGCTC CATCGTAGTC 
ATCCACCCTG ACACAGGCCG CGAGCTGTCC CCGGAGGAAG CCCACCGTGC CGGGCTCATT 
GACTGGAACA TGTTCGTGAA ACTCAGAAGC CAGGAGTGCG ACTGGGAGGA GATCTCAGTG 
AAGGGTCCCA ATGGGGAGTC CTCAGTGATA CACGACAGGA AGTCTGGCAA GAAGTTCTCC 
ATCGAAGAGG CCCTGCAGAG TGGCAGGCTG ACCCCTGCTC AGTATGACCG CTATGTCAAC 
AAGGATATGT CCATCCAGGA GCTGGCGGTC TTGGTATCTG GGCAGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPL ( XM_006720902.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006720965.1 Homo sapiens periplakin (PPL), transcript variant X3, mRNA.

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CloneID	OHu48890
Clone ID Related Accession (Same CDS sequence)	XM_006720902.4 , XM_006720902.3
Accession Version	XM_006720902.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	periplakin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006720902.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGGTA CGGGGGTCCA GGAACCGGGA ACTGAACCCG GAGTGCGGGA CTCTCGGGAA 
CGCGGGACCG AGGCCACCCT GAAGCTGGGA AGCGCCGCCA GCATCTCTAA CAAGGAGCTC 
TCGGAGCTGA TCGAGCAGCT GCAGAAGAAT GCCGACCAGG TGGAGAAGAA CATCGTGGAC 
ACAGAGGCCA AGATGCAGAG TGACCTGGCT CGGCTGCAGG AGGGTCGGCA GCCTGAGCAC 
CGGGACGTGA CCCTGCAGAA GGTGTTGGAC TCTGAGAAGC TGCTCTATGT GCTAGAGGCG 
GATGCGGCCA TTGCCAAGCA CATGAAGCAC CCACAGGGGG ACATGATCGC CGAGGATATC 
CGCCAGCTGA AGGAGCGTGT GACCAACCTG CGCGGGAAAC ACAAGCAGAT CTACAGGCTG 
GCGGTGAAGG AAGTGGATCC ACAGGTCAAC TGGGCGGCAC TGGTGGAGGA GAAGCTGGAC 
AAGCTGAACA ACCAGAGCTT TGGGACTGAC CTGCCGCTGG TGGACCACCA AGTGGAGGAG 
CATAACATCT TCCACAATGA GGTCAAGGCC ATCGGGCCCC ACCTGGCCAA GGACGGGGAC 
AAGGAGCAGA ACAGCGAACT CCGGGCCAAG TACCAGAAAC TGCTGGCAGC ATCACAGGCC 
CGGCAGCAGC ACCTGAGTTC GCTGCAGGAC TACATGCAGC GCTGCACCAA TGAGCTGTAC 
TGGCTGGACC AGCAGGCCAA GGGCCGCATG CAGTACGACT GGAGTGACCG CAACCTCGAC 
TACCCCAGCC GCCGGCGCCA GTATGAGAAT TTCATCAACC GGAACCTGGA GGCCAAAGAG 
GAGAGAATCA ACAAACTGCA CAGCGAGGGC GACCAGCTGC TGGCGGCCGA GCACCCCGGG 
AGGAACTCCA TTGAGGCGCA CATGGAGGCT GTGCACGCAG ACTGGAAGGA GTACCTGAAC 
CTGCTCATCT GCGAGGAGAG CCACCTCAAG TACATGGAGG ACTACCACCA GTTTCACGAA 
GACGTGAAGG ACGCTCAGGA GCTGCTGCGC AAGGTGGACT CGGACCTGAA CCAGAAGTAT 
GGCCCTGACT TCAAGGACCG GTACCAGATT GAGCTGCTGC TGCGGGAGCT GGATGACCAG 
GAGAAGGTGC TGGACAAGTA TGAGGACGTG GTGCAGGGGC TGCAGAAGCG AGGCCAGCAG 
GTGGTGCCCC TCAAGTACCG CCGGGAGACT CCGCTCAAGC CCATCCCCGT GGAGGCACTC 
TGTGACTTTG AGGGGGAGCA GGGCCTGATC TCGCGGGGCT ACAGCTACAC CCTGCAGAAG 
AACAACGGGG AGAGCTGGGA GCTCATGGAC AGCGCTGGGA ACAAGCTGAT TGCTCCGGCC 
GTGTGTTTTG TGATCCCCCC CACAGACCCT GAGGCCCTGG CTCTGGCTGA CAGCCTGGGC 
AGCCAGTACC GGAGCGTGCG GCAGAAGGCA GCTGGGAGCA AACGCACGCT GCAGCAGCGG 
TATGAGGTGC TGAAGACCGA GAATCCCGGA GATGCCTCTG ACCTACAGGG GCGGCAGCTG 
CTGGCTGGCT TGGACAAGGT GGCCAGCGAC CTGGACCGGC AGGAGAAGGC CATCACAGGG 
ATCCTGCGGC CACCACTGGA GCAAGGCCGG GCTGTGCAGG ACAGTGCCGA GCGGGCCAAG 
GACCTCAAGA ACATCACCAA CGAGCTACTG CGGATTGAAC CTGAGAAGAC GCGGAGCACG 
GCTGAGGGCG AAGCCTTCAT CCAGGCCCTC CCAGGCAGTG GCACCACACC CCTGCTGAGG 
ACCCGGGTGG AGGACACCAA CCGGAAATAC GAGCACCTCC TGCAGCTGCT GGACTTGGCC 
CAGGAGAAGG TTGATGTGGC CAACCGCCTG GAGAAGAGCC TGCAGCAGAG CTGGGAGTTG 
CTGGCCACAC ACGAGAACCA TCTGAATCAG GATGACACAG TGCCTGAGAG CAGCCGTGTC 
CTGGACAGCA AGGGGCAGGA GCTGGCGGCC ATGGCCTGTG AGTTACAGGC CCAGAAGTCC 
CTCCTGGGTG AGGTGGAGCA GAACTTGCAG GCGGCCAAGC AGTGCTCGAG CACACTGGCC 
AGCCGCTTCC AGGAGCACTG TCCGGACCTG GAGCGCCAGG AGGCCGAGGT GCACAAGCTG 
GGCCAGCGTT TCAACAACCT GCGCCAGCAG GTGGAACGCA GGGCGCAGAG CCTACAGAGC 
GCCAAGGCAG CCTACGAGCA CTTCCACCGC GGCCATGACC ACGTGCTGCA GTTCCTAGTC 
AGCATCCCCA GTTACGAGCC CCAGGAGACA GACAGCCTCA GCCAGATGGA GACCAAGCTG 
AAGAACCAGA AGAACCTGCT AGATGAGATA GCAAGTAGGG AGCAGGAAGT ACAGAAGATC 
TGTGCCAATT CCCAGCAGTA CCAGCAAGCT GTAAAGGACT ATGAGTTAGA AGCAGAAAAA 
CTAAGGTCTC TTCTCGACTT GGAGAATGGA AGGAGAAGCC ACGTGAGCAA GAGAGCCAGG 
CTCCAATCTC CTGCCACCAA AGTGAAGGAA GAGGAAGCAG CACTTGCCGC CAAGTTCACT 
GAAGTTTATG CCATCAACAG ACAGAGGCTG CAGAATCTGG AGTTTGCTCT GAATCTCCTC 
AGACAGCAGC CGGAAGTAGA AGTGACCCAT GAGACCCTGC AAAGGAATAG GCCGGACTCT 
GGAGTGGAGG AGGCGTGGAA GATCAGGAAG GAACTGGATG AGGAGACTGA GCGGAGGCGG 
CAGCTGGAGA ACGAGGTCAA GAGCACCCAG GAAGAAATCT GGACCTTGAG GAATCAGGGG 
CCTCAGGAAT CGGTGGTGAG GAAGGAGGTG CTCAAGAAGG TGCCGGATCC CGTGCTGGAG 
GAGAGCTTCC AGCAGCTGCA GCGGACGCTG GCAGAGGAGC AGCACAAGAA CCAGCTGCTG 
CAGGAGGAGC TGGAGGCACT GCAGCTGCAG CTGCGTGCCC TGGAGCAGGA GACCAGAGAC 
GGGGGGCAGG AGTACGTGGT CAAGGAGGTC CTGCGCATCG AGCCTGACAG GGCCCAGGCG 
GATGAGGTCT TGCAGCTGCG GGAGGAGCTG GAGGCACTGA GGCGGCAGAA GGGCGCCCGG 
GAGGCAGAGG TGCTCCTCCT GCAGCAGCGT GTGGCCGCCC TGGCTGAAGA GAAGAGCCGG 
GCGCAGGAGA AGGTCACAGA GAAAGAGGTG GTGAAACTGC AGAATGACCC CCAGCTGGAG 
GCAGAGTACC AGCAGCTGCA GGAGGACCAC CAGCGCCAGG ACCAGCTCAG GGAGAAGCAG 
GAGGAGGAGC TGAGCTTCCT CCAGGACAAG CTCAAGAGGC TAGAGAAGGA GCGGGCCATG 
GCCGAGGGCA AGATCACCGT CAAGGAGGTG CTCAAGGTGG AGAAGGACGC GGCCACCGAG 
AGGGAGGTCA GCGATCTCAC CCGCCAATAT GAGGACGAGG CTGCCAAGGC TCGCGCTAGC 
CAGAGGGAGA AGACGGAGCT GCTCCGAAAG ATATGGGCCT TGGAGGAGGA GAACGCCAAA 
GTGGTGGTGC AGGAGAAGGT GCGGGAGATC GTGCGGCCAG ACCCCAAGGC GGAAAGTGAA 
GTGGCGAACC TCCGCCTGGA GCTTGTGGAG CAGGAGCGAA AGTACCGGGG TGCCGAGGAG 
CAGCTCCGGA GCTACCAGAG TGAGCTGGAG GCCCTCAGGA GGCGAGGCCC CCAGGTGGAA 
GTCAAAGAGG TGACTAAGGA AGTCATTAAG TACAAGACTG ACCCTGAGAT GGAGAAGGAG 
CTTCAGCGGC TCAGGGAGGA GATCGTGGAC AAGACCAGAC TGATCGAAAG GTGTGATTTA 
GAGATCTACC AGCTGAAAAA GGAAATCCAG GCCCTGAAAG ACACCAAACC CCAGGTCCAG 
ACCAAAGAGG TGGTCCAGGA GATCCTCCAA TTCCAAGAAG ACCCTCAAAC CAAGGAGGAG 
GTGGCGTCTC TGAGGGCAAA GCTCTCAGAG GAGCAGAAGA AACAAGTGGA TCTGGAGAGG 
GAAAGAGCTT CCCAGGAAGA GCAGATCGCC CGGAAAGAGG AGGAGCTCTC GCGGGTGAAG 
GAAAGGGTGG TGCAGCAGGA GGTGGTCAGG TATGAGGAGG AGCCAGGCCT GCGGGCCGAG 
GCGAGCGCCT TTGCCGAGAG CATCGATGTG GAGCTGCGGC AGATTGACAA GCTGCGGGCA 
GAGCTGCGGC GGCTGCAGCG CCGGCGCACC GAGCTTGAGC GGCAGCTGGA GGAGCTAGAG 
CGCGAGCGGC AGGCCCGCAG GGAGGCCGAG CGCGAGGTAC AGCGGTTGCA GCAGCGGCTG 
GCAGCGCTGG AGCAGGAAGA AGCTGAGGCC CGTGAGAAGG TAACCCATAC GCAGAAGGTG 
GTGCTGCAGC AGGACCCGCA GCAGGCGCGA GAGCATGCCC TGCTCCGACT CCAGCTGGAA 
GAAGAGCAGC ACCGGCGGCA GCTCCTGGAG GGGGAGCTCG AGACCCTCCG GAGGAAACTG 
GCTGCACTGG AGAAGGCGGA GGTCAAGGAG AAGGTGGTGC TCTCCGAGAG TGTCCAGGTG 
GAGAAGGGCG ACACCGAGCA AGAGATCCAG AGGCTCAAGA GCAGCCTGGA GGAGGAGAGC 
CGCAGCAAGC GCGAGCTGGA CGTCGAGGTG AGCCGGCTGG AAGCCAGGCT TTCGGAGCTG 
GAATTCCATA ACTCCAAGTC ATCCAAGGAA CTAGACTTTC TGAGGGAAGA GAACCACAAA 
TTACAGCTGG AGAGGCAAAA CCTGCAGCTG GAGACCCGAA GGCTCCAATC GGAAATCAAC 
ATGGCAGCGA CGGAAACACG AGACCTGCGG AACATGACCG TGGCGGACTC TGGGACCAAC 
CATGACTCCA GACTGTGGTC CCTGGAGAGG GAACTGGATG ACCTCAAGAG GCTCTCCAAG 
GACAAAGACC TCGAGATCGA CGAGCTGCAG AAGCGCCTGG GCTCCGTGGC CGTCAAGCGG 
GAGCAGCGGG AGAACCACCT GCGGCGCTCC ATCGTAGTCA TCCACCCTGA CACAGGCCGC 
GAGCTGTCCC CGGAGGAAGC CCACCGTGCC GGGCTCATTG ACTGGAACAT GTTCGTGAAA 
CTCAGAAGCC AGGAGTGCGA CTGGGAGGAG ATCTCAGTGA AGGGTCCCAA TGGGGAGTCC 
TCAGTGATAC ACGACAGGAA GTCTGGCAAG AAGTTCTCCA TCGAAGAGGC CCTGCAGAGT 
GGCAGGCTGA CCCCTGCTCA GTATGACCGC TATGTCAACA AGGATATGTC CATCCAGGAG 
CTGGCGGTCT TGGTATCTGG GCAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006720965.1
CDS	16..5325
Translation	MGGTGVQEPGTEPGVRDSRERGTEATLKLGSAASISNKELSELIEQLQKNADQVEKNIVDTEAKMQSDLARLQEGRQPEHRDVTLQKVLDSEKLLYVLEADAAIAKHMKHPQGDMIAEDIRQLKERVTNLRGKHKQIYRLAVKEVDPQVNWAALVEEKLDKLNNQSFGTDLPLVDHQVEEHNIFHNEVKAIGPHLAKDGDKEQNSELRAKYQKLLAASQARQQHLSSLQDYMQRCTNELYWLDQQAKGRMQYDWSDRNLDYPSRRRQYENFINRNLEAKEERINKLHSEGDQLLAAEHPGRNSIEAHMEAVHADWKEYLNLLICEESHLKYMEDYHQFHEDVKDAQELLRKVDSDLNQKYGPDFKDRYQIELLLRELDDQEKVLDKYEDVVQGLQKRGQQVVPLKYRRETPLKPIPVEALCDFEGEQGLISRGYSYTLQKNNGESWELMDSAGNKLIAPAVCFVIPPTDPEALALADSLGSQYRSVRQKAAGSKRTLQQRYEVLKTENPGDASDLQGRQLLAGLDKVASDLDRQEKAITGILRPPLEQGRAVQDSAERAKDLKNITNELLRIEPEKTRSTAEGEAFIQALPGSGTTPLLRTRVEDTNRKYEHLLQLLDLAQEKVDVANRLEKSLQQSWELLATHENHLNQDDTVPESSRVLDSKGQELAAMACELQAQKSLLGEVEQNLQAAKQCSSTLASRFQEHCPDLERQEAEVHKLGQRFNNLRQQVERRAQSLQSAKAAYEHFHRGHDHVLQFLVSIPSYEPQETDSLSQMETKLKNQKNLLDEIASREQEVQKICANSQQYQQAVKDYELEAEKLRSLLDLENGRRSHVSKRARLQSPATKVKEEEAALAAKFTEVYAINRQRLQNLEFALNLLRQQPEVEVTHETLQRNRPDSGVEEAWKIRKELDEETERRRQLENEVKSTQEEIWTLRNQGPQESVVRKEVLKKVPDPVLEESFQQLQRTLAEEQHKNQLLQEELEALQLQLRALEQETRDGGQEYVVKEVLRIEPDRAQADEVLQLREELEALRRQKGAREAEVLLLQQRVAALAEEKSRAQEKVTEKEVVKLQNDPQLEAEYQQLQEDHQRQDQLREKQEEELSFLQDKLKRLEKERAMAEGKITVKEVLKVEKDAATEREVSDLTRQYEDEAAKARASQREKTELLRKIWALEEENAKVVVQEKVREIVRPDPKAESEVANLRLELVEQERKYRGAEEQLRSYQSELEALRRRGPQVEVKEVTKEVIKYKTDPEMEKELQRLREEIVDKTRLIERCDLEIYQLKKEIQALKDTKPQVQTKEVVQEILQFQEDPQTKEEVASLRAKLSEEQKKQVDLERERASQEEQIARKEEELSRVKERVVQQEVVRYEEEPGLRAEASAFAESIDVELRQIDKLRAELRRLQRRRTELERQLEELERERQARREAEREVQRLQQRLAALEQEEAEAREKVTHTQKVVLQQDPQQAREHALLRLQLEEEQHRRQLLEGELETLRRKLAALEKAEVKEKVVLSESVQVEKGDTEQEIQRLKSSLEEESRSKRELDVEVSRLEARLSELEFHNSKSSKELDFLREENHKLQLERQNLQLETRRLQSEINMAATETRDLRNMTVADSGTNHDSRLWSLERELDDLKRLSKDKDLEIDELQKRLGSVAVKREQRENHLRRSIVVIHPDTGRELSPEEAHRAGLIDWNMFVKLRSQECDWEEISVKGPNGESSVIHDRKSGKKFSIEEALQSGRLTPAQYDRYVNKDMSIQELAVLVSGQK

Target ORF information:

RefSeq Version	XM_006720902.4
Organism	Homo sapiens(human)
Definition	Homo sapiens periplakin (PPL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006720902.4

ORF Insert Sequence:

ATGGGGGGTA CGGGGGTCCA GGAACCGGGA ACTGAACCCG GAGTGCGGGA CTCTCGGGAA 
CGCGGGACCG AGGCCACCCT GAAGCTGGGA AGCGCCGCCA GCATCTCTAA CAAGGAGCTC 
TCGGAGCTGA TCGAGCAGCT GCAGAAGAAT GCCGACCAGG TGGAGAAGAA CATCGTGGAC 
ACAGAGGCCA AGATGCAGAG TGACCTGGCT CGGCTGCAGG AGGGTCGGCA GCCTGAGCAC 
CGGGACGTGA CCCTGCAGAA GGTGTTGGAC TCTGAGAAGC TGCTCTATGT GCTAGAGGCG 
GATGCGGCCA TTGCCAAGCA CATGAAGCAC CCACAGGGGG ACATGATCGC CGAGGATATC 
CGCCAGCTGA AGGAGCGTGT GACCAACCTG CGCGGGAAAC ACAAGCAGAT CTACAGGCTG 
GCGGTGAAGG AAGTGGATCC ACAGGTCAAC TGGGCGGCAC TGGTGGAGGA GAAGCTGGAC 
AAGCTGAACA ACCAGAGCTT TGGGACTGAC CTGCCGCTGG TGGACCACCA AGTGGAGGAG 
CATAACATCT TCCACAATGA GGTCAAGGCC ATCGGGCCCC ACCTGGCCAA GGACGGGGAC 
AAGGAGCAGA ACAGCGAACT CCGGGCCAAG TACCAGAAAC TGCTGGCAGC ATCACAGGCC 
CGGCAGCAGC ACCTGAGTTC GCTGCAGGAC TACATGCAGC GCTGCACCAA TGAGCTGTAC 
TGGCTGGACC AGCAGGCCAA GGGCCGCATG CAGTACGACT GGAGTGACCG CAACCTCGAC 
TACCCCAGCC GCCGGCGCCA GTATGAGAAT TTCATCAACC GGAACCTGGA GGCCAAAGAG 
GAGAGAATCA ACAAACTGCA CAGCGAGGGC GACCAGCTGC TGGCGGCCGA GCACCCCGGG 
AGGAACTCCA TTGAGGCGCA CATGGAGGCT GTGCACGCAG ACTGGAAGGA GTACCTGAAC 
CTGCTCATCT GCGAGGAGAG CCACCTCAAG TACATGGAGG ACTACCACCA GTTTCACGAA 
GACGTGAAGG ACGCTCAGGA GCTGCTGCGC AAGGTGGACT CGGACCTGAA CCAGAAGTAT 
GGCCCTGACT TCAAGGACCG GTACCAGATT GAGCTGCTGC TGCGGGAGCT GGATGACCAG 
GAGAAGGTGC TGGACAAGTA TGAGGACGTG GTGCAGGGGC TGCAGAAGCG AGGCCAGCAG 
GTGGTGCCCC TCAAGTACCG CCGGGAGACT CCGCTCAAGC CCATCCCCGT GGAGGCACTC 
TGTGACTTTG AGGGGGAGCA GGGCCTGATC TCGCGGGGCT ACAGCTACAC CCTGCAGAAG 
AACAACGGGG AGAGCTGGGA GCTCATGGAC AGCGCTGGGA ACAAGCTGAT TGCTCCGGCC 
GTGTGTTTTG TGATCCCCCC CACAGACCCT GAGGCCCTGG CTCTGGCTGA CAGCCTGGGC 
AGCCAGTACC GGAGCGTGCG GCAGAAGGCA GCTGGGAGCA AACGCACGCT GCAGCAGCGG 
TATGAGGTGC TGAAGACCGA GAATCCCGGA GATGCCTCTG ACCTACAGGG GCGGCAGCTG 
CTGGCTGGCT TGGACAAGGT GGCCAGCGAC CTGGACCGGC AGGAGAAGGC CATCACAGGG 
ATCCTGCGGC CACCACTGGA GCAAGGCCGG GCTGTGCAGG ACAGTGCCGA GCGGGCCAAG 
GACCTCAAGA ACATCACCAA CGAGCTACTG CGGATTGAAC CTGAGAAGAC GCGGAGCACG 
GCTGAGGGCG AAGCCTTCAT CCAGGCCCTC CCAGGCAGTG GCACCACACC CCTGCTGAGG 
ACCCGGGTGG AGGACACCAA CCGGAAATAC GAGCACCTCC TGCAGCTGCT GGACTTGGCC 
CAGGAGAAGG TTGATGTGGC CAACCGCCTG GAGAAGAGCC TGCAGCAGAG CTGGGAGTTG 
CTGGCCACAC ACGAGAACCA TCTGAATCAG GATGACACAG TGCCTGAGAG CAGCCGTGTC 
CTGGACAGCA AGGGGCAGGA GCTGGCGGCC ATGGCCTGTG AGTTACAGGC CCAGAAGTCC 
CTCCTGGGTG AGGTGGAGCA GAACTTGCAG GCGGCCAAGC AGTGCTCGAG CACACTGGCC 
AGCCGCTTCC AGGAGCACTG TCCGGACCTG GAGCGCCAGG AGGCCGAGGT GCACAAGCTG 
GGCCAGCGTT TCAACAACCT GCGCCAGCAG GTGGAACGCA GGGCGCAGAG CCTACAGAGC 
GCCAAGGCAG CCTACGAGCA CTTCCACCGC GGCCATGACC ACGTGCTGCA GTTCCTAGTC 
AGCATCCCCA GTTACGAGCC CCAGGAGACA GACAGCCTCA GCCAGATGGA GACCAAGCTG 
AAGAACCAGA AGAACCTGCT AGATGAGATA GCAAGTAGGG AGCAGGAAGT ACAGAAGATC 
TGTGCCAATT CCCAGCAGTA CCAGCAAGCT GTAAAGGACT ATGAGTTAGA AGCAGAAAAA 
CTAAGGTCTC TTCTCGACTT GGAGAATGGA AGGAGAAGCC ACGTGAGCAA GAGAGCCAGG 
CTCCAATCTC CTGCCACCAA AGTGAAGGAA GAGGAAGCAG CACTTGCCGC CAAGTTCACT 
GAAGTTTATG CCATCAACAG ACAGAGGCTG CAGAATCTGG AGTTTGCTCT GAATCTCCTC 
AGACAGCAGC CGGAAGTAGA AGTGACCCAT GAGACCCTGC AAAGGAATAG GCCGGACTCT 
GGAGTGGAGG AGGCGTGGAA GATCAGGAAG GAACTGGATG AGGAGACTGA GCGGAGGCGG 
CAGCTGGAGA ACGAGGTCAA GAGCACCCAG GAAGAAATCT GGACCTTGAG GAATCAGGGG 
CCTCAGGAAT CGGTGGTGAG GAAGGAGGTG CTCAAGAAGG TGCCGGATCC CGTGCTGGAG 
GAGAGCTTCC AGCAGCTGCA GCGGACGCTG GCAGAGGAGC AGCACAAGAA CCAGCTGCTG 
CAGGAGGAGC TGGAGGCACT GCAGCTGCAG CTGCGTGCCC TGGAGCAGGA GACCAGAGAC 
GGGGGGCAGG AGTACGTGGT CAAGGAGGTC CTGCGCATCG AGCCTGACAG GGCCCAGGCG 
GATGAGGTCT TGCAGCTGCG GGAGGAGCTG GAGGCACTGA GGCGGCAGAA GGGCGCCCGG 
GAGGCAGAGG TGCTCCTCCT GCAGCAGCGT GTGGCCGCCC TGGCTGAAGA GAAGAGCCGG 
GCGCAGGAGA AGGTCACAGA GAAAGAGGTG GTGAAACTGC AGAATGACCC CCAGCTGGAG 
GCAGAGTACC AGCAGCTGCA GGAGGACCAC CAGCGCCAGG ACCAGCTCAG GGAGAAGCAG 
GAGGAGGAGC TGAGCTTCCT CCAGGACAAG CTCAAGAGGC TAGAGAAGGA GCGGGCCATG 
GCCGAGGGCA AGATCACCGT CAAGGAGGTG CTCAAGGTGG AGAAGGACGC GGCCACCGAG 
AGGGAGGTCA GCGATCTCAC CCGCCAATAT GAGGACGAGG CTGCCAAGGC TCGCGCTAGC 
CAGAGGGAGA AGACGGAGCT GCTCCGAAAG ATATGGGCCT TGGAGGAGGA GAACGCCAAA 
GTGGTGGTGC AGGAGAAGGT GCGGGAGATC GTGCGGCCAG ACCCCAAGGC GGAAAGTGAA 
GTGGCGAACC TCCGCCTGGA GCTTGTGGAG CAGGAGCGAA AGTACCGGGG TGCCGAGGAG 
CAGCTCCGGA GCTACCAGAG TGAGCTGGAG GCCCTCAGGA GGCGAGGCCC CCAGGTGGAA 
GTCAAAGAGG TGACTAAGGA AGTCATTAAG TACAAGACTG ACCCTGAGAT GGAGAAGGAG 
CTTCAGCGGC TCAGGGAGGA GATCGTGGAC AAGACCAGAC TGATCGAAAG GTGTGATTTA 
GAGATCTACC AGCTGAAAAA GGAAATCCAG GCCCTGAAAG ACACCAAACC CCAGGTCCAG 
ACCAAAGAGG TGGTCCAGGA GATCCTCCAA TTCCAAGAAG ACCCTCAAAC CAAGGAGGAG 
GTGGCGTCTC TGAGGGCAAA GCTCTCAGAG GAGCAGAAGA AACAAGTGGA TCTGGAGAGG 
GAAAGAGCTT CCCAGGAAGA GCAGATCGCC CGGAAAGAGG AGGAGCTCTC GCGGGTGAAG 
GAAAGGGTGG TGCAGCAGGA GGTGGTCAGG TATGAGGAGG AGCCAGGCCT GCGGGCCGAG 
GCGAGCGCCT TTGCCGAGAG CATCGATGTG GAGCTGCGGC AGATTGACAA GCTGCGGGCA 
GAGCTGCGGC GGCTGCAGCG CCGGCGCACC GAGCTTGAGC GGCAGCTGGA GGAGCTAGAG 
CGCGAGCGGC AGGCCCGCAG GGAGGCCGAG CGCGAGGTAC AGCGGTTGCA GCAGCGGCTG 
GCAGCGCTGG AGCAGGAAGA AGCTGAGGCC CGTGAGAAGG TAACCCATAC GCAGAAGGTG 
GTGCTGCAGC AGGACCCGCA GCAGGCGCGA GAGCATGCCC TGCTCCGACT CCAGCTGGAA 
GAAGAGCAGC ACCGGCGGCA GCTCCTGGAG GGGGAGCTCG AGACCCTCCG GAGGAAACTG 
GCTGCACTGG AGAAGGCGGA GGTCAAGGAG AAGGTGGTGC TCTCCGAGAG TGTCCAGGTG 
GAGAAGGGCG ACACCGAGCA AGAGATCCAG AGGCTCAAGA GCAGCCTGGA GGAGGAGAGC 
CGCAGCAAGC GCGAGCTGGA CGTCGAGGTG AGCCGGCTGG AAGCCAGGCT TTCGGAGCTG 
GAATTCCATA ACTCCAAGTC ATCCAAGGAA CTAGACTTTC TGAGGGAAGA GAACCACAAA 
TTACAGCTGG AGAGGCAAAA CCTGCAGCTG GAGACCCGAA GGCTCCAATC GGAAATCAAC 
ATGGCAGCGA CGGAAACACG AGACCTGCGG AACATGACCG TGGCGGACTC TGGGACCAAC 
CATGACTCCA GACTGTGGTC CCTGGAGAGG GAACTGGATG ACCTCAAGAG GCTCTCCAAG 
GACAAAGACC TCGAGATCGA CGAGCTGCAG AAGCGCCTGG GCTCCGTGGC CGTCAAGCGG 
GAGCAGCGGG AGAACCACCT GCGGCGCTCC ATCGTAGTCA TCCACCCTGA CACAGGCCGC 
GAGCTGTCCC CGGAGGAAGC CCACCGTGCC GGGCTCATTG ACTGGAACAT GTTCGTGAAA 
CTCAGAAGCC AGGAGTGCGA CTGGGAGGAG ATCTCAGTGA AGGGTCCCAA TGGGGAGTCC 
TCAGTGATAC ACGACAGGAA GTCTGGCAAG AAGTTCTCCA TCGAAGAGGC CCTGCAGAGT 
GGCAGGCTGA CCCCTGCTCA GTATGACCGC TATGTCAACA AGGATATGTC CATCCAGGAG 
CTGGCGGTCT TGGTATCTGG GCAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPL ( XM_006720902.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006720965.1 Homo sapiens periplakin (PPL), transcript variant X3, mRNA.

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CloneID	OHu48890
Clone ID Related Accession (Same CDS sequence)	XM_006720902.4 , XM_006720902.3
Accession Version	XM_006720902.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	periplakin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006720902.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGGTA CGGGGGTCCA GGAACCGGGA ACTGAACCCG GAGTGCGGGA CTCTCGGGAA 
CGCGGGACCG AGGCCACCCT GAAGCTGGGA AGCGCCGCCA GCATCTCTAA CAAGGAGCTC 
TCGGAGCTGA TCGAGCAGCT GCAGAAGAAT GCCGACCAGG TGGAGAAGAA CATCGTGGAC 
ACAGAGGCCA AGATGCAGAG TGACCTGGCT CGGCTGCAGG AGGGTCGGCA GCCTGAGCAC 
CGGGACGTGA CCCTGCAGAA GGTGTTGGAC TCTGAGAAGC TGCTCTATGT GCTAGAGGCG 
GATGCGGCCA TTGCCAAGCA CATGAAGCAC CCACAGGGGG ACATGATCGC CGAGGATATC 
CGCCAGCTGA AGGAGCGTGT GACCAACCTG CGCGGGAAAC ACAAGCAGAT CTACAGGCTG 
GCGGTGAAGG AAGTGGATCC ACAGGTCAAC TGGGCGGCAC TGGTGGAGGA GAAGCTGGAC 
AAGCTGAACA ACCAGAGCTT TGGGACTGAC CTGCCGCTGG TGGACCACCA AGTGGAGGAG 
CATAACATCT TCCACAATGA GGTCAAGGCC ATCGGGCCCC ACCTGGCCAA GGACGGGGAC 
AAGGAGCAGA ACAGCGAACT CCGGGCCAAG TACCAGAAAC TGCTGGCAGC ATCACAGGCC 
CGGCAGCAGC ACCTGAGTTC GCTGCAGGAC TACATGCAGC GCTGCACCAA TGAGCTGTAC 
TGGCTGGACC AGCAGGCCAA GGGCCGCATG CAGTACGACT GGAGTGACCG CAACCTCGAC 
TACCCCAGCC GCCGGCGCCA GTATGAGAAT TTCATCAACC GGAACCTGGA GGCCAAAGAG 
GAGAGAATCA ACAAACTGCA CAGCGAGGGC GACCAGCTGC TGGCGGCCGA GCACCCCGGG 
AGGAACTCCA TTGAGGCGCA CATGGAGGCT GTGCACGCAG ACTGGAAGGA GTACCTGAAC 
CTGCTCATCT GCGAGGAGAG CCACCTCAAG TACATGGAGG ACTACCACCA GTTTCACGAA 
GACGTGAAGG ACGCTCAGGA GCTGCTGCGC AAGGTGGACT CGGACCTGAA CCAGAAGTAT 
GGCCCTGACT TCAAGGACCG GTACCAGATT GAGCTGCTGC TGCGGGAGCT GGATGACCAG 
GAGAAGGTGC TGGACAAGTA TGAGGACGTG GTGCAGGGGC TGCAGAAGCG AGGCCAGCAG 
GTGGTGCCCC TCAAGTACCG CCGGGAGACT CCGCTCAAGC CCATCCCCGT GGAGGCACTC 
TGTGACTTTG AGGGGGAGCA GGGCCTGATC TCGCGGGGCT ACAGCTACAC CCTGCAGAAG 
AACAACGGGG AGAGCTGGGA GCTCATGGAC AGCGCTGGGA ACAAGCTGAT TGCTCCGGCC 
GTGTGTTTTG TGATCCCCCC CACAGACCCT GAGGCCCTGG CTCTGGCTGA CAGCCTGGGC 
AGCCAGTACC GGAGCGTGCG GCAGAAGGCA GCTGGGAGCA AACGCACGCT GCAGCAGCGG 
TATGAGGTGC TGAAGACCGA GAATCCCGGA GATGCCTCTG ACCTACAGGG GCGGCAGCTG 
CTGGCTGGCT TGGACAAGGT GGCCAGCGAC CTGGACCGGC AGGAGAAGGC CATCACAGGG 
ATCCTGCGGC CACCACTGGA GCAAGGCCGG GCTGTGCAGG ACAGTGCCGA GCGGGCCAAG 
GACCTCAAGA ACATCACCAA CGAGCTACTG CGGATTGAAC CTGAGAAGAC GCGGAGCACG 
GCTGAGGGCG AAGCCTTCAT CCAGGCCCTC CCAGGCAGTG GCACCACACC CCTGCTGAGG 
ACCCGGGTGG AGGACACCAA CCGGAAATAC GAGCACCTCC TGCAGCTGCT GGACTTGGCC 
CAGGAGAAGG TTGATGTGGC CAACCGCCTG GAGAAGAGCC TGCAGCAGAG CTGGGAGTTG 
CTGGCCACAC ACGAGAACCA TCTGAATCAG GATGACACAG TGCCTGAGAG CAGCCGTGTC 
CTGGACAGCA AGGGGCAGGA GCTGGCGGCC ATGGCCTGTG AGTTACAGGC CCAGAAGTCC 
CTCCTGGGTG AGGTGGAGCA GAACTTGCAG GCGGCCAAGC AGTGCTCGAG CACACTGGCC 
AGCCGCTTCC AGGAGCACTG TCCGGACCTG GAGCGCCAGG AGGCCGAGGT GCACAAGCTG 
GGCCAGCGTT TCAACAACCT GCGCCAGCAG GTGGAACGCA GGGCGCAGAG CCTACAGAGC 
GCCAAGGCAG CCTACGAGCA CTTCCACCGC GGCCATGACC ACGTGCTGCA GTTCCTAGTC 
AGCATCCCCA GTTACGAGCC CCAGGAGACA GACAGCCTCA GCCAGATGGA GACCAAGCTG 
AAGAACCAGA AGAACCTGCT AGATGAGATA GCAAGTAGGG AGCAGGAAGT ACAGAAGATC 
TGTGCCAATT CCCAGCAGTA CCAGCAAGCT GTAAAGGACT ATGAGTTAGA AGCAGAAAAA 
CTAAGGTCTC TTCTCGACTT GGAGAATGGA AGGAGAAGCC ACGTGAGCAA GAGAGCCAGG 
CTCCAATCTC CTGCCACCAA AGTGAAGGAA GAGGAAGCAG CACTTGCCGC CAAGTTCACT 
GAAGTTTATG CCATCAACAG ACAGAGGCTG CAGAATCTGG AGTTTGCTCT GAATCTCCTC 
AGACAGCAGC CGGAAGTAGA AGTGACCCAT GAGACCCTGC AAAGGAATAG GCCGGACTCT 
GGAGTGGAGG AGGCGTGGAA GATCAGGAAG GAACTGGATG AGGAGACTGA GCGGAGGCGG 
CAGCTGGAGA ACGAGGTCAA GAGCACCCAG GAAGAAATCT GGACCTTGAG GAATCAGGGG 
CCTCAGGAAT CGGTGGTGAG GAAGGAGGTG CTCAAGAAGG TGCCGGATCC CGTGCTGGAG 
GAGAGCTTCC AGCAGCTGCA GCGGACGCTG GCAGAGGAGC AGCACAAGAA CCAGCTGCTG 
CAGGAGGAGC TGGAGGCACT GCAGCTGCAG CTGCGTGCCC TGGAGCAGGA GACCAGAGAC 
GGGGGGCAGG AGTACGTGGT CAAGGAGGTC CTGCGCATCG AGCCTGACAG GGCCCAGGCG 
GATGAGGTCT TGCAGCTGCG GGAGGAGCTG GAGGCACTGA GGCGGCAGAA GGGCGCCCGG 
GAGGCAGAGG TGCTCCTCCT GCAGCAGCGT GTGGCCGCCC TGGCTGAAGA GAAGAGCCGG 
GCGCAGGAGA AGGTCACAGA GAAAGAGGTG GTGAAACTGC AGAATGACCC CCAGCTGGAG 
GCAGAGTACC AGCAGCTGCA GGAGGACCAC CAGCGCCAGG ACCAGCTCAG GGAGAAGCAG 
GAGGAGGAGC TGAGCTTCCT CCAGGACAAG CTCAAGAGGC TAGAGAAGGA GCGGGCCATG 
GCCGAGGGCA AGATCACCGT CAAGGAGGTG CTCAAGGTGG AGAAGGACGC GGCCACCGAG 
AGGGAGGTCA GCGATCTCAC CCGCCAATAT GAGGACGAGG CTGCCAAGGC TCGCGCTAGC 
CAGAGGGAGA AGACGGAGCT GCTCCGAAAG ATATGGGCCT TGGAGGAGGA GAACGCCAAA 
GTGGTGGTGC AGGAGAAGGT GCGGGAGATC GTGCGGCCAG ACCCCAAGGC GGAAAGTGAA 
GTGGCGAACC TCCGCCTGGA GCTTGTGGAG CAGGAGCGAA AGTACCGGGG TGCCGAGGAG 
CAGCTCCGGA GCTACCAGAG TGAGCTGGAG GCCCTCAGGA GGCGAGGCCC CCAGGTGGAA 
GTCAAAGAGG TGACTAAGGA AGTCATTAAG TACAAGACTG ACCCTGAGAT GGAGAAGGAG 
CTTCAGCGGC TCAGGGAGGA GATCGTGGAC AAGACCAGAC TGATCGAAAG GTGTGATTTA 
GAGATCTACC AGCTGAAAAA GGAAATCCAG GCCCTGAAAG ACACCAAACC CCAGGTCCAG 
ACCAAAGAGG TGGTCCAGGA GATCCTCCAA TTCCAAGAAG ACCCTCAAAC CAAGGAGGAG 
GTGGCGTCTC TGAGGGCAAA GCTCTCAGAG GAGCAGAAGA AACAAGTGGA TCTGGAGAGG 
GAAAGAGCTT CCCAGGAAGA GCAGATCGCC CGGAAAGAGG AGGAGCTCTC GCGGGTGAAG 
GAAAGGGTGG TGCAGCAGGA GGTGGTCAGG TATGAGGAGG AGCCAGGCCT GCGGGCCGAG 
GCGAGCGCCT TTGCCGAGAG CATCGATGTG GAGCTGCGGC AGATTGACAA GCTGCGGGCA 
GAGCTGCGGC GGCTGCAGCG CCGGCGCACC GAGCTTGAGC GGCAGCTGGA GGAGCTAGAG 
CGCGAGCGGC AGGCCCGCAG GGAGGCCGAG CGCGAGGTAC AGCGGTTGCA GCAGCGGCTG 
GCAGCGCTGG AGCAGGAAGA AGCTGAGGCC CGTGAGAAGG TAACCCATAC GCAGAAGGTG 
GTGCTGCAGC AGGACCCGCA GCAGGCGCGA GAGCATGCCC TGCTCCGACT CCAGCTGGAA 
GAAGAGCAGC ACCGGCGGCA GCTCCTGGAG GGGGAGCTCG AGACCCTCCG GAGGAAACTG 
GCTGCACTGG AGAAGGCGGA GGTCAAGGAG AAGGTGGTGC TCTCCGAGAG TGTCCAGGTG 
GAGAAGGGCG ACACCGAGCA AGAGATCCAG AGGCTCAAGA GCAGCCTGGA GGAGGAGAGC 
CGCAGCAAGC GCGAGCTGGA CGTCGAGGTG AGCCGGCTGG AAGCCAGGCT TTCGGAGCTG 
GAATTCCATA ACTCCAAGTC ATCCAAGGAA CTAGACTTTC TGAGGGAAGA GAACCACAAA 
TTACAGCTGG AGAGGCAAAA CCTGCAGCTG GAGACCCGAA GGCTCCAATC GGAAATCAAC 
ATGGCAGCGA CGGAAACACG AGACCTGCGG AACATGACCG TGGCGGACTC TGGGACCAAC 
CATGACTCCA GACTGTGGTC CCTGGAGAGG GAACTGGATG ACCTCAAGAG GCTCTCCAAG 
GACAAAGACC TCGAGATCGA CGAGCTGCAG AAGCGCCTGG GCTCCGTGGC CGTCAAGCGG 
GAGCAGCGGG AGAACCACCT GCGGCGCTCC ATCGTAGTCA TCCACCCTGA CACAGGCCGC 
GAGCTGTCCC CGGAGGAAGC CCACCGTGCC GGGCTCATTG ACTGGAACAT GTTCGTGAAA 
CTCAGAAGCC AGGAGTGCGA CTGGGAGGAG ATCTCAGTGA AGGGTCCCAA TGGGGAGTCC 
TCAGTGATAC ACGACAGGAA GTCTGGCAAG AAGTTCTCCA TCGAAGAGGC CCTGCAGAGT 
GGCAGGCTGA CCCCTGCTCA GTATGACCGC TATGTCAACA AGGATATGTC CATCCAGGAG 
CTGGCGGTCT TGGTATCTGG GCAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006720965.1
CDS	16..5325
Translation	MGGTGVQEPGTEPGVRDSRERGTEATLKLGSAASISNKELSELIEQLQKNADQVEKNIVDTEAKMQSDLARLQEGRQPEHRDVTLQKVLDSEKLLYVLEADAAIAKHMKHPQGDMIAEDIRQLKERVTNLRGKHKQIYRLAVKEVDPQVNWAALVEEKLDKLNNQSFGTDLPLVDHQVEEHNIFHNEVKAIGPHLAKDGDKEQNSELRAKYQKLLAASQARQQHLSSLQDYMQRCTNELYWLDQQAKGRMQYDWSDRNLDYPSRRRQYENFINRNLEAKEERINKLHSEGDQLLAAEHPGRNSIEAHMEAVHADWKEYLNLLICEESHLKYMEDYHQFHEDVKDAQELLRKVDSDLNQKYGPDFKDRYQIELLLRELDDQEKVLDKYEDVVQGLQKRGQQVVPLKYRRETPLKPIPVEALCDFEGEQGLISRGYSYTLQKNNGESWELMDSAGNKLIAPAVCFVIPPTDPEALALADSLGSQYRSVRQKAAGSKRTLQQRYEVLKTENPGDASDLQGRQLLAGLDKVASDLDRQEKAITGILRPPLEQGRAVQDSAERAKDLKNITNELLRIEPEKTRSTAEGEAFIQALPGSGTTPLLRTRVEDTNRKYEHLLQLLDLAQEKVDVANRLEKSLQQSWELLATHENHLNQDDTVPESSRVLDSKGQELAAMACELQAQKSLLGEVEQNLQAAKQCSSTLASRFQEHCPDLERQEAEVHKLGQRFNNLRQQVERRAQSLQSAKAAYEHFHRGHDHVLQFLVSIPSYEPQETDSLSQMETKLKNQKNLLDEIASREQEVQKICANSQQYQQAVKDYELEAEKLRSLLDLENGRRSHVSKRARLQSPATKVKEEEAALAAKFTEVYAINRQRLQNLEFALNLLRQQPEVEVTHETLQRNRPDSGVEEAWKIRKELDEETERRRQLENEVKSTQEEIWTLRNQGPQESVVRKEVLKKVPDPVLEESFQQLQRTLAEEQHKNQLLQEELEALQLQLRALEQETRDGGQEYVVKEVLRIEPDRAQADEVLQLREELEALRRQKGAREAEVLLLQQRVAALAEEKSRAQEKVTEKEVVKLQNDPQLEAEYQQLQEDHQRQDQLREKQEEELSFLQDKLKRLEKERAMAEGKITVKEVLKVEKDAATEREVSDLTRQYEDEAAKARASQREKTELLRKIWALEEENAKVVVQEKVREIVRPDPKAESEVANLRLELVEQERKYRGAEEQLRSYQSELEALRRRGPQVEVKEVTKEVIKYKTDPEMEKELQRLREEIVDKTRLIERCDLEIYQLKKEIQALKDTKPQVQTKEVVQEILQFQEDPQTKEEVASLRAKLSEEQKKQVDLERERASQEEQIARKEEELSRVKERVVQQEVVRYEEEPGLRAEASAFAESIDVELRQIDKLRAELRRLQRRRTELERQLEELERERQARREAEREVQRLQQRLAALEQEEAEAREKVTHTQKVVLQQDPQQAREHALLRLQLEEEQHRRQLLEGELETLRRKLAALEKAEVKEKVVLSESVQVEKGDTEQEIQRLKSSLEEESRSKRELDVEVSRLEARLSELEFHNSKSSKELDFLREENHKLQLERQNLQLETRRLQSEINMAATETRDLRNMTVADSGTNHDSRLWSLERELDDLKRLSKDKDLEIDELQKRLGSVAVKREQRENHLRRSIVVIHPDTGRELSPEEAHRAGLIDWNMFVKLRSQECDWEEISVKGPNGESSVIHDRKSGKKFSIEEALQSGRLTPAQYDRYVNKDMSIQELAVLVSGQK

Target ORF information:

RefSeq Version	XM_006720902.3
Organism	Homo sapiens(human)
Definition	Homo sapiens periplakin (PPL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006720902.3

ORF Insert Sequence:

ATGGGGGGTA CGGGGGTCCA GGAACCGGGA ACTGAACCCG GAGTGCGGGA CTCTCGGGAA 
CGCGGGACCG AGGCCACCCT GAAGCTGGGA AGCGCCGCCA GCATCTCTAA CAAGGAGCTC 
TCGGAGCTGA TCGAGCAGCT GCAGAAGAAT GCCGACCAGG TGGAGAAGAA CATCGTGGAC 
ACAGAGGCCA AGATGCAGAG TGACCTGGCT CGGCTGCAGG AGGGTCGGCA GCCTGAGCAC 
CGGGACGTGA CCCTGCAGAA GGTGTTGGAC TCTGAGAAGC TGCTCTATGT GCTAGAGGCG 
GATGCGGCCA TTGCCAAGCA CATGAAGCAC CCACAGGGGG ACATGATCGC CGAGGATATC 
CGCCAGCTGA AGGAGCGTGT GACCAACCTG CGCGGGAAAC ACAAGCAGAT CTACAGGCTG 
GCGGTGAAGG AAGTGGATCC ACAGGTCAAC TGGGCGGCAC TGGTGGAGGA GAAGCTGGAC 
AAGCTGAACA ACCAGAGCTT TGGGACTGAC CTGCCGCTGG TGGACCACCA AGTGGAGGAG 
CATAACATCT TCCACAATGA GGTCAAGGCC ATCGGGCCCC ACCTGGCCAA GGACGGGGAC 
AAGGAGCAGA ACAGCGAACT CCGGGCCAAG TACCAGAAAC TGCTGGCAGC ATCACAGGCC 
CGGCAGCAGC ACCTGAGTTC GCTGCAGGAC TACATGCAGC GCTGCACCAA TGAGCTGTAC 
TGGCTGGACC AGCAGGCCAA GGGCCGCATG CAGTACGACT GGAGTGACCG CAACCTCGAC 
TACCCCAGCC GCCGGCGCCA GTATGAGAAT TTCATCAACC GGAACCTGGA GGCCAAAGAG 
GAGAGAATCA ACAAACTGCA CAGCGAGGGC GACCAGCTGC TGGCGGCCGA GCACCCCGGG 
AGGAACTCCA TTGAGGCGCA CATGGAGGCT GTGCACGCAG ACTGGAAGGA GTACCTGAAC 
CTGCTCATCT GCGAGGAGAG CCACCTCAAG TACATGGAGG ACTACCACCA GTTTCACGAA 
GACGTGAAGG ACGCTCAGGA GCTGCTGCGC AAGGTGGACT CGGACCTGAA CCAGAAGTAT 
GGCCCTGACT TCAAGGACCG GTACCAGATT GAGCTGCTGC TGCGGGAGCT GGATGACCAG 
GAGAAGGTGC TGGACAAGTA TGAGGACGTG GTGCAGGGGC TGCAGAAGCG AGGCCAGCAG 
GTGGTGCCCC TCAAGTACCG CCGGGAGACT CCGCTCAAGC CCATCCCCGT GGAGGCACTC 
TGTGACTTTG AGGGGGAGCA GGGCCTGATC TCGCGGGGCT ACAGCTACAC CCTGCAGAAG 
AACAACGGGG AGAGCTGGGA GCTCATGGAC AGCGCTGGGA ACAAGCTGAT TGCTCCGGCC 
GTGTGTTTTG TGATCCCCCC CACAGACCCT GAGGCCCTGG CTCTGGCTGA CAGCCTGGGC 
AGCCAGTACC GGAGCGTGCG GCAGAAGGCA GCTGGGAGCA AACGCACGCT GCAGCAGCGG 
TATGAGGTGC TGAAGACCGA GAATCCCGGA GATGCCTCTG ACCTACAGGG GCGGCAGCTG 
CTGGCTGGCT TGGACAAGGT GGCCAGCGAC CTGGACCGGC AGGAGAAGGC CATCACAGGG 
ATCCTGCGGC CACCACTGGA GCAAGGCCGG GCTGTGCAGG ACAGTGCCGA GCGGGCCAAG 
GACCTCAAGA ACATCACCAA CGAGCTACTG CGGATTGAAC CTGAGAAGAC GCGGAGCACG 
GCTGAGGGCG AAGCCTTCAT CCAGGCCCTC CCAGGCAGTG GCACCACACC CCTGCTGAGG 
ACCCGGGTGG AGGACACCAA CCGGAAATAC GAGCACCTCC TGCAGCTGCT GGACTTGGCC 
CAGGAGAAGG TTGATGTGGC CAACCGCCTG GAGAAGAGCC TGCAGCAGAG CTGGGAGTTG 
CTGGCCACAC ACGAGAACCA TCTGAATCAG GATGACACAG TGCCTGAGAG CAGCCGTGTC 
CTGGACAGCA AGGGGCAGGA GCTGGCGGCC ATGGCCTGTG AGTTACAGGC CCAGAAGTCC 
CTCCTGGGTG AGGTGGAGCA GAACTTGCAG GCGGCCAAGC AGTGCTCGAG CACACTGGCC 
AGCCGCTTCC AGGAGCACTG TCCGGACCTG GAGCGCCAGG AGGCCGAGGT GCACAAGCTG 
GGCCAGCGTT TCAACAACCT GCGCCAGCAG GTGGAACGCA GGGCGCAGAG CCTACAGAGC 
GCCAAGGCAG CCTACGAGCA CTTCCACCGC GGCCATGACC ACGTGCTGCA GTTCCTAGTC 
AGCATCCCCA GTTACGAGCC CCAGGAGACA GACAGCCTCA GCCAGATGGA GACCAAGCTG 
AAGAACCAGA AGAACCTGCT AGATGAGATA GCAAGTAGGG AGCAGGAAGT ACAGAAGATC 
TGTGCCAATT CCCAGCAGTA CCAGCAAGCT GTAAAGGACT ATGAGTTAGA AGCAGAAAAA 
CTAAGGTCTC TTCTCGACTT GGAGAATGGA AGGAGAAGCC ACGTGAGCAA GAGAGCCAGG 
CTCCAATCTC CTGCCACCAA AGTGAAGGAA GAGGAAGCAG CACTTGCCGC CAAGTTCACT 
GAAGTTTATG CCATCAACAG ACAGAGGCTG CAGAATCTGG AGTTTGCTCT GAATCTCCTC 
AGACAGCAGC CGGAAGTAGA AGTGACCCAT GAGACCCTGC AAAGGAATAG GCCGGACTCT 
GGAGTGGAGG AGGCGTGGAA GATCAGGAAG GAACTGGATG AGGAGACTGA GCGGAGGCGG 
CAGCTGGAGA ACGAGGTCAA GAGCACCCAG GAAGAAATCT GGACCTTGAG GAATCAGGGG 
CCTCAGGAAT CGGTGGTGAG GAAGGAGGTG CTCAAGAAGG TGCCGGATCC CGTGCTGGAG 
GAGAGCTTCC AGCAGCTGCA GCGGACGCTG GCAGAGGAGC AGCACAAGAA CCAGCTGCTG 
CAGGAGGAGC TGGAGGCACT GCAGCTGCAG CTGCGTGCCC TGGAGCAGGA GACCAGAGAC 
GGGGGGCAGG AGTACGTGGT CAAGGAGGTC CTGCGCATCG AGCCTGACAG GGCCCAGGCG 
GATGAGGTCT TGCAGCTGCG GGAGGAGCTG GAGGCACTGA GGCGGCAGAA GGGCGCCCGG 
GAGGCAGAGG TGCTCCTCCT GCAGCAGCGT GTGGCCGCCC TGGCTGAAGA GAAGAGCCGG 
GCGCAGGAGA AGGTCACAGA GAAAGAGGTG GTGAAACTGC AGAATGACCC CCAGCTGGAG 
GCAGAGTACC AGCAGCTGCA GGAGGACCAC CAGCGCCAGG ACCAGCTCAG GGAGAAGCAG 
GAGGAGGAGC TGAGCTTCCT CCAGGACAAG CTCAAGAGGC TAGAGAAGGA GCGGGCCATG 
GCCGAGGGCA AGATCACCGT CAAGGAGGTG CTCAAGGTGG AGAAGGACGC GGCCACCGAG 
AGGGAGGTCA GCGATCTCAC CCGCCAATAT GAGGACGAGG CTGCCAAGGC TCGCGCTAGC 
CAGAGGGAGA AGACGGAGCT GCTCCGAAAG ATATGGGCCT TGGAGGAGGA GAACGCCAAA 
GTGGTGGTGC AGGAGAAGGT GCGGGAGATC GTGCGGCCAG ACCCCAAGGC GGAAAGTGAA 
GTGGCGAACC TCCGCCTGGA GCTTGTGGAG CAGGAGCGAA AGTACCGGGG TGCCGAGGAG 
CAGCTCCGGA GCTACCAGAG TGAGCTGGAG GCCCTCAGGA GGCGAGGCCC CCAGGTGGAA 
GTCAAAGAGG TGACTAAGGA AGTCATTAAG TACAAGACTG ACCCTGAGAT GGAGAAGGAG 
CTTCAGCGGC TCAGGGAGGA GATCGTGGAC AAGACCAGAC TGATCGAAAG GTGTGATTTA 
GAGATCTACC AGCTGAAAAA GGAAATCCAG GCCCTGAAAG ACACCAAACC CCAGGTCCAG 
ACCAAAGAGG TGGTCCAGGA GATCCTCCAA TTCCAAGAAG ACCCTCAAAC CAAGGAGGAG 
GTGGCGTCTC TGAGGGCAAA GCTCTCAGAG GAGCAGAAGA AACAAGTGGA TCTGGAGAGG 
GAAAGAGCTT CCCAGGAAGA GCAGATCGCC CGGAAAGAGG AGGAGCTCTC GCGGGTGAAG 
GAAAGGGTGG TGCAGCAGGA GGTGGTCAGG TATGAGGAGG AGCCAGGCCT GCGGGCCGAG 
GCGAGCGCCT TTGCCGAGAG CATCGATGTG GAGCTGCGGC AGATTGACAA GCTGCGGGCA 
GAGCTGCGGC GGCTGCAGCG CCGGCGCACC GAGCTTGAGC GGCAGCTGGA GGAGCTAGAG 
CGCGAGCGGC AGGCCCGCAG GGAGGCCGAG CGCGAGGTAC AGCGGTTGCA GCAGCGGCTG 
GCAGCGCTGG AGCAGGAAGA AGCTGAGGCC CGTGAGAAGG TAACCCATAC GCAGAAGGTG 
GTGCTGCAGC AGGACCCGCA GCAGGCGCGA GAGCATGCCC TGCTCCGACT CCAGCTGGAA 
GAAGAGCAGC ACCGGCGGCA GCTCCTGGAG GGGGAGCTCG AGACCCTCCG GAGGAAACTG 
GCTGCACTGG AGAAGGCGGA GGTCAAGGAG AAGGTGGTGC TCTCCGAGAG TGTCCAGGTG 
GAGAAGGGCG ACACCGAGCA AGAGATCCAG AGGCTCAAGA GCAGCCTGGA GGAGGAGAGC 
CGCAGCAAGC GCGAGCTGGA CGTCGAGGTG AGCCGGCTGG AAGCCAGGCT TTCGGAGCTG 
GAATTCCATA ACTCCAAGTC ATCCAAGGAA CTAGACTTTC TGAGGGAAGA GAACCACAAA 
TTACAGCTGG AGAGGCAAAA CCTGCAGCTG GAGACCCGAA GGCTCCAATC GGAAATCAAC 
ATGGCAGCGA CGGAAACACG AGACCTGCGG AACATGACCG TGGCGGACTC TGGGACCAAC 
CATGACTCCA GACTGTGGTC CCTGGAGAGG GAACTGGATG ACCTCAAGAG GCTCTCCAAG 
GACAAAGACC TCGAGATCGA CGAGCTGCAG AAGCGCCTGG GCTCCGTGGC CGTCAAGCGG 
GAGCAGCGGG AGAACCACCT GCGGCGCTCC ATCGTAGTCA TCCACCCTGA CACAGGCCGC 
GAGCTGTCCC CGGAGGAAGC CCACCGTGCC GGGCTCATTG ACTGGAACAT GTTCGTGAAA 
CTCAGAAGCC AGGAGTGCGA CTGGGAGGAG ATCTCAGTGA AGGGTCCCAA TGGGGAGTCC 
TCAGTGATAC ACGACAGGAA GTCTGGCAAG AAGTTCTCCA TCGAAGAGGC CCTGCAGAGT 
GGCAGGCTGA CCCCTGCTCA GTATGACCGC TATGTCAACA AGGATATGTC CATCCAGGAG 
CTGGCGGTCT TGGTATCTGG GCAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPL ( XM_017023374.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878863.1 Homo sapiens periplakin (PPL), transcript variant X1, mRNA.

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CloneID	OHu86120
Clone ID Related Accession (Same CDS sequence)	XM_017023374.1 , XM_017023374.2
Accession Version	XM_017023374.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	periplakin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGGTA CGGGGGTCCA GGAACCGGGA ACTGAACCCG GAGTGCGGGA CTCTCGGGAA 
CGCGGGACCG AGGCCACCCT GAAGCTGGGA AGCGCCGCCA GCATCTCTAA CAAGGAGCTC 
TCGGAGCTGA TCGAGCAGCT GCAGAAGAAT GCCGACCAGG TGGAGAAGAA CATCGTGGAC 
ACAGAGGCCA AGATGCAGAG TGACCTGGCT CGGCTGCAGG AGGGTCGGCA GCCTGAGCAC 
CGGGACGTGA CCCTGCAGAA GGTGTTGGAC TCTGAGAAGC TGCTCTATGT GCTAGAGGCG 
GATGCGGCCA TTGCCAAGCA CATGAAGCAC CCACAGGGGG ACATGATCGC CGAGGATATC 
CGCCAGCTGA AGGAGCGTGT GACCAACCTG CGCGGGAAAC ACAAGCAGAT CTACAGGCTG 
GCGGTGAAGG AAGTGGATCC ACAGGTCAAC TGGGCGGCAC TGGTGGAGGA GAAGCTGGAC 
AAGCTGAACA ACCAGAGCTT TGGGACTGAC CTGCCGCTGG TGGACCACCA AGTGGAGGAG 
CATAACATCT TCCACAATGA GGTCAAGGCC ATCGGGCCCC ACCTGGCCAA GGACGGGGAC 
AAGGAGCAGA ACAGCGAACT CCGGGCCAAG TACCAGAAAC TGCTGGCAGC ATCACAGGCC 
CGGCAGCAGC ACCTGAGTTC GCTGCAGGAC TACATGCAGC GCTGCACCAA TGAGCTGTAC 
TGGCTGGACC AGCAGGCCAA GGGCCGCATG CAGTACGACT GGAGTGACCG CAACCTCGAC 
TACCCCAGCC GCCGGCGCCA GTATGAGAAT TTCATCAACC GGAACCTGGA GGCCAAAGAG 
GAGAGAATCA ACAAACTGCA CAGCGAGGGC GACCAGCTGC TGGCGGCCGA GCACCCCGGG 
AGGAACTCCA TTGAGGCGCA CATGGAGGCT GTGCACGCAG ACTGGAAGGA GTACCTGAAC 
CTGCTCATCT GCGAGGAGAG CCACCTCAAG TACATGGAGG ACTACCACCA GTTTCACGAA 
GACGTGAAGG ACGCTCAGGA GCTGCTGCGC AAGGTGGACT CGGACCTGAA CCAGAAGTAT 
GGCCCTGACT TCAAGGACCG GTACCAGATT GAGCTGCTGC TGCGGGAGCT GGATGACCAG 
GAGAAGGTGC TGGACAAGTA TGAGGACGTG GTGCAGGGGC TGCAGAAGCG AGGCCAGCAG 
GTGGTGCCCC TCAAGTACCG CCGGGAGACT CCGCTCAAGC CCATCCCCGT GGAGGCACTC 
TGTGACTTTG AGGGGGAGCA GGCCCTCAGG CCCTGCCGGG ATCCTGACTG TCCAGCCTGC 
CCCCGCCAGG GCCTGATCTC GCGGGGCTAC AGCTACACCC TGCAGAAGAA CAACGGGGAG 
AGCTGGGAGC TCATGGACAG CGCTGGGAAC AAGCTGATTG CTCCGGCCGT GTGTTTTGTG 
ATCCCCCCCA CAGACCCTGA GGCCCTGGCT CTGGCTGACA GCCTGGGCAG CCAGTACCGG 
AGCGTGCGGC AGAAGGCAGC TGGGAGCAAA CGCACGCTGC AGCAGCGGTA TGAGGTGCTG 
AAGACCGAGA ATCCCGGAGA TGCCTCTGAC CTACAGGGGC GGCAGCTGCT GGCTGGCTTG 
GACAAGGTGG CCAGCGACCT GGACCGGCAG GAGAAGGCCA TCACAGGGAT CCTGCGGCCA 
CCACTGGAGC AAGGCCGGGC TGTGCAGGAC AGTGCCGAGC GGGCCAAGGA CCTCAAGAAC 
ATCACCAACG AGCTACTGCG GATTGAACCT GAGAAGACGC GGAGCACGGC TGAGGGCGAA 
GCCTTCATCC AGGCCCTCCC AGGCAGTGGC ACCACACCCC TGCTGAGGAC CCGGGTGGAG 
GACACCAACC GGAAATACGA GCACCTCCTG CAGCTGCTGG ACTTGGCCCA GGAGAAGGTT 
GATGTGGCCA ACCGCCTGGA GAAGAGCCTG CAGCAGAGCT GGGAGTTGCT GGCCACACAC 
GAGAACCATC TGAATCAGGA TGACACAGTG CCTGAGAGCA GCCGTGTCCT GGACAGCAAG 
GGGCAGGAGC TGGCGGCCAT GGCCTGTGAG TTACAGGCCC AGAAGTCCCT CCTGGGTGAG 
GTGGAGCAGA ACTTGCAGGC GGCCAAGCAG TGCTCGAGCA CACTGGCCAG CCGCTTCCAG 
GAGCACTGTC CGGACCTGGA GCGCCAGGAG GCCGAGGTGC ACAAGCTGGG CCAGCGTTTC 
AACAACCTGC GCCAGCAGGT GGAACGCAGG GCGCAGAGCC TACAGAGCGC CAAGGCAGCC 
TACGAGCACT TCCACCGCGG CCATGACCAC GTGCTGCAGT TCCTAGTCAG CATCCCCAGT 
TACGAGCCCC AGGAGACAGA CAGCCTCAGC CAGATGGAGA CCAAGCTGAA GAACCAGAAG 
AACCTGCTAG ATGAGATAGC AAGTAGGGAG CAGGAAGTAC AGAAGATCTG TGCCAATTCC 
CAGCAGTACC AGCAAGCTGT AAAGGACTAT GAGTTAGAAG CAGAAAAACT AAGGTCTCTT 
CTCGACTTGG AGAATGGAAG GAGAAGCCAC GTGAGCAAGA GAGCCAGGCT CCAATCTCCT 
GCCACCAAAG TGAAGGAAGA GGAAGCAGCA CTTGCCGCCA AGTTCACTGA AGTTTATGCC 
ATCAACAGAC AGAGGCTGCA GAATCTGGAG TTTGCTCTGA ATCTCCTCAG ACAGCAGCCG 
GAAGTAGAAG TGACCCATGA GACCCTGCAA AGGAATAGGC CGGACTCTGG AGTGGAGGAG 
GCGTGGAAGA TCAGGAAGGA ACTGGATGAG GAGACTGAGC GGAGGCGGCA GCTGGAGAAC 
GAGGTCAAGA GCACCCAGGA AGAAATCTGG ACCTTGAGGA ATCAGGGGCC TCAGGAATCG 
GTGGTGAGGA AGGAGGTGCT CAAGAAGGTG CCGGATCCCG TGCTGGAGGA GAGCTTCCAG 
CAGCTGCAGC GGACGCTGGC AGAGGAGCAG CACAAGAACC AGCTGCTGCA GGAGGAGCTG 
GAGGCACTGC AGCTGCAGCT GCGTGCCCTG GAGCAGGAGA CCAGAGACGG GGGGCAGGAG 
TACGTGGTCA AGGAGGTCCT GCGCATCGAG CCTGACAGGG CCCAGGCGGA TGAGGTCTTG 
CAGCTGCGGG AGGAGCTGGA GGCACTGAGG CGGCAGAAGG GCGCCCGGGA GGCAGAGGTG 
CTCCTCCTGC AGCAGCGTGT GGCCGCCCTG GCTGAAGAGA AGAGCCGGGC GCAGGAGAAG 
GTCACAGAGA AAGAGGTGGT GAAACTGCAG AATGACCCCC AGCTGGAGGC AGAGTACCAG 
CAGCTGCAGG AGGACCACCA GCGCCAGGAC CAGCTCAGGG AGAAGCAGGA GGAGGAGCTG 
AGCTTCCTCC AGGACAAGCT CAAGAGGCTA GAGAAGGAGC GGGCCATGGC CGAGGGCAAG 
ATCACCGTCA AGGAGGTGCT CAAGGTGGAG AAGGACGCGG CCACCGAGAG GGAGGTCAGC 
GATCTCACCC GCCAATATGA GGACGAGGCT GCCAAGGCTC GCGCTAGCCA GAGGGAGAAG 
ACGGAGCTGC TCCGAAAGAT ATGGGCCTTG GAGGAGGAGA ACGCCAAAGT GGTGGTGCAG 
GAGAAGGTGC GGGAGATCGT GCGGCCAGAC CCCAAGGCGG AAAGTGAAGT GGCGAACCTC 
CGCCTGGAGC TTGTGGAGCA GGAGCGAAAG TACCGGGGTG CCGAGGAGCA GCTCCGGAGC 
TACCAGAGTG AGCTGGAGGC CCTCAGGAGG CGAGGCCCCC AGGTGGAAGT CAAAGAGGTG 
ACTAAGGAAG TCATTAAGTA CAAGACTGAC CCTGAGATGG AGAAGGAGCT TCAGCGGCTC 
AGGGAGGAGA TCGTGGACAA GACCAGACTG ATCGAAAGGT GTGATTTAGA GATCTACCAG 
CTGAAAAAGG AAATCCAGGC CCTGAAAGAC ACCAAACCCC AGGTCCAGAC CAAAGAGGTG 
GTCCAGGAGA TCCTCCAATT CCAAGAAGAC CCTCAAACCA AGGAGGAGGT GGCGTCTCTG 
AGGGCAAAGC TCTCAGAGGA GCAGAAGAAA CAAGTGGATC TGGAGAGGGA AAGAGCTTCC 
CAGGAAGAGC AGATCGCCCG GAAAGAGGAG GAGCTCTCGC GGGTGAAGGA AAGGGTGGTG 
CAGCAGGAGG TGGTCAGGTA TGAGGAGGAG CCAGGCCTGC GGGCCGAGGC GAGCGCCTTT 
GCCGAGAGCA TCGATGTGGA GCTGCGGCAG ATTGACAAGC TGCGGGCAGA GCTGCGGCGG 
CTGCAGCGCC GGCGCACCGA GCTTGAGCGG CAGCTGGAGG AGCTAGAGCG CGAGCGGCAG 
GCCCGCAGGG AGGCCGAGCG CGAGGTACAG CGGTTGCAGC AGCGGCTGGC AGCGCTGGAG 
CAGGAAGAAG CTGAGGCCCG TGAGAAGGTA ACCCATACGC AGAAGGTGGT GCTGCAGCAG 
GACCCGCAGC AGGCGCGAGA GCATGCCCTG CTCCGACTCC AGCTGGAAGA AGAGCAGCAC 
CGGCGGCAGC TCCTGGAGGG GGAGCTCGAG ACCCTCCGGA GGAAACTGGC TGCACTGGAG 
AAGGCGGAGG TCAAGGAGAA GGTGGTGCTC TCCGAGAGTG TCCAGGTGGA GAAGGGCGAC 
ACCGAGCAAG AGATCCAGAG GCTCAAGAGC AGCCTGGAGG AGGAGAGCCG CAGCAAGCGC 
GAGCTGGACG TCGAGGTGAG CCGGCTGGAA GCCAGGCTTT CGGAGCTGGA ATTCCATAAC 
TCCAAGTCAT CCAAGGAACT AGACTTTCTG AGGGAAGAGA ACCACAAATT ACAGCTGGAG 
AGGCAAAACC TGCAGCTGGA GACCCGAAGG CTCCAATCGG AAATCAACAT GGCAGCGACG 
GAAACACGAG ACCTGCGGAA CATGACCGTG GCGGACTCTG GGACCAACCA TGACTCCAGA 
CTGTGGTCCC TGGAGAGGGA ACTGGATGAC CTCAAGAGGC TCTCCAAGGA CAAAGACCTC 
GAGATCGACG AGCTGCAGAA GCGCCTGGGC TCCGTGGCCG TCAAGCGGGA GCAGCGGGAG 
AACCACCTGC GGCGCTCCAT CGTAGTCATC CACCCTGACA CAGGCCGCGA GCTGTCCCCG 
GAGGAAGCCC ACCGTGCCGG GCTCATTGAC TGGAACATGT TCGTGAAACT CAGAAGCCAG 
GAGTGCGACT GGGAGGAGAT CTCAGTGAAG GGTCCCAATG GGGAGTCCTC AGTGATACAC 
GACAGGAAGT CTGGCAAGAA GTTCTCCATC GAAGAGGCCC TGCAGAGTGG CAGGCTGACC 
CCTGCTCAGT ATGACCGCTA TGTCAACAAG GATATGTCCA TCCAGGAGCT GGCGGTCTTG 
GTATCTGGGC AGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878863.1
CDS	16..5373
Translation	MGGTGVQEPGTEPGVRDSRERGTEATLKLGSAASISNKELSELIEQLQKNADQVEKNIVDTEAKMQSDLARLQEGRQPEHRDVTLQKVLDSEKLLYVLEADAAIAKHMKHPQGDMIAEDIRQLKERVTNLRGKHKQIYRLAVKEVDPQVNWAALVEEKLDKLNNQSFGTDLPLVDHQVEEHNIFHNEVKAIGPHLAKDGDKEQNSELRAKYQKLLAASQARQQHLSSLQDYMQRCTNELYWLDQQAKGRMQYDWSDRNLDYPSRRRQYENFINRNLEAKEERINKLHSEGDQLLAAEHPGRNSIEAHMEAVHADWKEYLNLLICEESHLKYMEDYHQFHEDVKDAQELLRKVDSDLNQKYGPDFKDRYQIELLLRELDDQEKVLDKYEDVVQGLQKRGQQVVPLKYRRETPLKPIPVEALCDFEGEQALRPCRDPDCPACPRQGLISRGYSYTLQKNNGESWELMDSAGNKLIAPAVCFVIPPTDPEALALADSLGSQYRSVRQKAAGSKRTLQQRYEVLKTENPGDASDLQGRQLLAGLDKVASDLDRQEKAITGILRPPLEQGRAVQDSAERAKDLKNITNELLRIEPEKTRSTAEGEAFIQALPGSGTTPLLRTRVEDTNRKYEHLLQLLDLAQEKVDVANRLEKSLQQSWELLATHENHLNQDDTVPESSRVLDSKGQELAAMACELQAQKSLLGEVEQNLQAAKQCSSTLASRFQEHCPDLERQEAEVHKLGQRFNNLRQQVERRAQSLQSAKAAYEHFHRGHDHVLQFLVSIPSYEPQETDSLSQMETKLKNQKNLLDEIASREQEVQKICANSQQYQQAVKDYELEAEKLRSLLDLENGRRSHVSKRARLQSPATKVKEEEAALAAKFTEVYAINRQRLQNLEFALNLLRQQPEVEVTHETLQRNRPDSGVEEAWKIRKELDEETERRRQLENEVKSTQEEIWTLRNQGPQESVVRKEVLKKVPDPVLEESFQQLQRTLAEEQHKNQLLQEELEALQLQLRALEQETRDGGQEYVVKEVLRIEPDRAQADEVLQLREELEALRRQKGAREAEVLLLQQRVAALAEEKSRAQEKVTEKEVVKLQNDPQLEAEYQQLQEDHQRQDQLREKQEEELSFLQDKLKRLEKERAMAEGKITVKEVLKVEKDAATEREVSDLTRQYEDEAAKARASQREKTELLRKIWALEEENAKVVVQEKVREIVRPDPKAESEVANLRLELVEQERKYRGAEEQLRSYQSELEALRRRGPQVEVKEVTKEVIKYKTDPEMEKELQRLREEIVDKTRLIERCDLEIYQLKKEIQALKDTKPQVQTKEVVQEILQFQEDPQTKEEVASLRAKLSEEQKKQVDLERERASQEEQIARKEEELSRVKERVVQQEVVRYEEEPGLRAEASAFAESIDVELRQIDKLRAELRRLQRRRTELERQLEELERERQARREAEREVQRLQQRLAALEQEEAEAREKVTHTQKVVLQQDPQQAREHALLRLQLEEEQHRRQLLEGELETLRRKLAALEKAEVKEKVVLSESVQVEKGDTEQEIQRLKSSLEEESRSKRELDVEVSRLEARLSELEFHNSKSSKELDFLREENHKLQLERQNLQLETRRLQSEINMAATETRDLRNMTVADSGTNHDSRLWSLERELDDLKRLSKDKDLEIDELQKRLGSVAVKREQRENHLRRSIVVIHPDTGRELSPEEAHRAGLIDWNMFVKLRSQECDWEEISVKGPNGESSVIHDRKSGKKFSIEEALQSGRLTPAQYDRYVNKDMSIQELAVLVSGQK

Target ORF information:

RefSeq Version	XM_017023374.1
Organism	Homo sapiens(human)
Definition	Homo sapiens periplakin (PPL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023374.1

ORF Insert Sequence:

ATGGGGGGTA CGGGGGTCCA GGAACCGGGA ACTGAACCCG GAGTGCGGGA CTCTCGGGAA 
CGCGGGACCG AGGCCACCCT GAAGCTGGGA AGCGCCGCCA GCATCTCTAA CAAGGAGCTC 
TCGGAGCTGA TCGAGCAGCT GCAGAAGAAT GCCGACCAGG TGGAGAAGAA CATCGTGGAC 
ACAGAGGCCA AGATGCAGAG TGACCTGGCT CGGCTGCAGG AGGGTCGGCA GCCTGAGCAC 
CGGGACGTGA CCCTGCAGAA GGTGTTGGAC TCTGAGAAGC TGCTCTATGT GCTAGAGGCG 
GATGCGGCCA TTGCCAAGCA CATGAAGCAC CCACAGGGGG ACATGATCGC CGAGGATATC 
CGCCAGCTGA AGGAGCGTGT GACCAACCTG CGCGGGAAAC ACAAGCAGAT CTACAGGCTG 
GCGGTGAAGG AAGTGGATCC ACAGGTCAAC TGGGCGGCAC TGGTGGAGGA GAAGCTGGAC 
AAGCTGAACA ACCAGAGCTT TGGGACTGAC CTGCCGCTGG TGGACCACCA AGTGGAGGAG 
CATAACATCT TCCACAATGA GGTCAAGGCC ATCGGGCCCC ACCTGGCCAA GGACGGGGAC 
AAGGAGCAGA ACAGCGAACT CCGGGCCAAG TACCAGAAAC TGCTGGCAGC ATCACAGGCC 
CGGCAGCAGC ACCTGAGTTC GCTGCAGGAC TACATGCAGC GCTGCACCAA TGAGCTGTAC 
TGGCTGGACC AGCAGGCCAA GGGCCGCATG CAGTACGACT GGAGTGACCG CAACCTCGAC 
TACCCCAGCC GCCGGCGCCA GTATGAGAAT TTCATCAACC GGAACCTGGA GGCCAAAGAG 
GAGAGAATCA ACAAACTGCA CAGCGAGGGC GACCAGCTGC TGGCGGCCGA GCACCCCGGG 
AGGAACTCCA TTGAGGCGCA CATGGAGGCT GTGCACGCAG ACTGGAAGGA GTACCTGAAC 
CTGCTCATCT GCGAGGAGAG CCACCTCAAG TACATGGAGG ACTACCACCA GTTTCACGAA 
GACGTGAAGG ACGCTCAGGA GCTGCTGCGC AAGGTGGACT CGGACCTGAA CCAGAAGTAT 
GGCCCTGACT TCAAGGACCG GTACCAGATT GAGCTGCTGC TGCGGGAGCT GGATGACCAG 
GAGAAGGTGC TGGACAAGTA TGAGGACGTG GTGCAGGGGC TGCAGAAGCG AGGCCAGCAG 
GTGGTGCCCC TCAAGTACCG CCGGGAGACT CCGCTCAAGC CCATCCCCGT GGAGGCACTC 
TGTGACTTTG AGGGGGAGCA GGCCCTCAGG CCCTGCCGGG ATCCTGACTG TCCAGCCTGC 
CCCCGCCAGG GCCTGATCTC GCGGGGCTAC AGCTACACCC TGCAGAAGAA CAACGGGGAG 
AGCTGGGAGC TCATGGACAG CGCTGGGAAC AAGCTGATTG CTCCGGCCGT GTGTTTTGTG 
ATCCCCCCCA CAGACCCTGA GGCCCTGGCT CTGGCTGACA GCCTGGGCAG CCAGTACCGG 
AGCGTGCGGC AGAAGGCAGC TGGGAGCAAA CGCACGCTGC AGCAGCGGTA TGAGGTGCTG 
AAGACCGAGA ATCCCGGAGA TGCCTCTGAC CTACAGGGGC GGCAGCTGCT GGCTGGCTTG 
GACAAGGTGG CCAGCGACCT GGACCGGCAG GAGAAGGCCA TCACAGGGAT CCTGCGGCCA 
CCACTGGAGC AAGGCCGGGC TGTGCAGGAC AGTGCCGAGC GGGCCAAGGA CCTCAAGAAC 
ATCACCAACG AGCTACTGCG GATTGAACCT GAGAAGACGC GGAGCACGGC TGAGGGCGAA 
GCCTTCATCC AGGCCCTCCC AGGCAGTGGC ACCACACCCC TGCTGAGGAC CCGGGTGGAG 
GACACCAACC GGAAATACGA GCACCTCCTG CAGCTGCTGG ACTTGGCCCA GGAGAAGGTT 
GATGTGGCCA ACCGCCTGGA GAAGAGCCTG CAGCAGAGCT GGGAGTTGCT GGCCACACAC 
GAGAACCATC TGAATCAGGA TGACACAGTG CCTGAGAGCA GCCGTGTCCT GGACAGCAAG 
GGGCAGGAGC TGGCGGCCAT GGCCTGTGAG TTACAGGCCC AGAAGTCCCT CCTGGGTGAG 
GTGGAGCAGA ACTTGCAGGC GGCCAAGCAG TGCTCGAGCA CACTGGCCAG CCGCTTCCAG 
GAGCACTGTC CGGACCTGGA GCGCCAGGAG GCCGAGGTGC ACAAGCTGGG CCAGCGTTTC 
AACAACCTGC GCCAGCAGGT GGAACGCAGG GCGCAGAGCC TACAGAGCGC CAAGGCAGCC 
TACGAGCACT TCCACCGCGG CCATGACCAC GTGCTGCAGT TCCTAGTCAG CATCCCCAGT 
TACGAGCCCC AGGAGACAGA CAGCCTCAGC CAGATGGAGA CCAAGCTGAA GAACCAGAAG 
AACCTGCTAG ATGAGATAGC AAGTAGGGAG CAGGAAGTAC AGAAGATCTG TGCCAATTCC 
CAGCAGTACC AGCAAGCTGT AAAGGACTAT GAGTTAGAAG CAGAAAAACT AAGGTCTCTT 
CTCGACTTGG AGAATGGAAG GAGAAGCCAC GTGAGCAAGA GAGCCAGGCT CCAATCTCCT 
GCCACCAAAG TGAAGGAAGA GGAAGCAGCA CTTGCCGCCA AGTTCACTGA AGTTTATGCC 
ATCAACAGAC AGAGGCTGCA GAATCTGGAG TTTGCTCTGA ATCTCCTCAG ACAGCAGCCG 
GAAGTAGAAG TGACCCATGA GACCCTGCAA AGGAATAGGC CGGACTCTGG AGTGGAGGAG 
GCGTGGAAGA TCAGGAAGGA ACTGGATGAG GAGACTGAGC GGAGGCGGCA GCTGGAGAAC 
GAGGTCAAGA GCACCCAGGA AGAAATCTGG ACCTTGAGGA ATCAGGGGCC TCAGGAATCG 
GTGGTGAGGA AGGAGGTGCT CAAGAAGGTG CCGGATCCCG TGCTGGAGGA GAGCTTCCAG 
CAGCTGCAGC GGACGCTGGC AGAGGAGCAG CACAAGAACC AGCTGCTGCA GGAGGAGCTG 
GAGGCACTGC AGCTGCAGCT GCGTGCCCTG GAGCAGGAGA CCAGAGACGG GGGGCAGGAG 
TACGTGGTCA AGGAGGTCCT GCGCATCGAG CCTGACAGGG CCCAGGCGGA TGAGGTCTTG 
CAGCTGCGGG AGGAGCTGGA GGCACTGAGG CGGCAGAAGG GCGCCCGGGA GGCAGAGGTG 
CTCCTCCTGC AGCAGCGTGT GGCCGCCCTG GCTGAAGAGA AGAGCCGGGC GCAGGAGAAG 
GTCACAGAGA AAGAGGTGGT GAAACTGCAG AATGACCCCC AGCTGGAGGC AGAGTACCAG 
CAGCTGCAGG AGGACCACCA GCGCCAGGAC CAGCTCAGGG AGAAGCAGGA GGAGGAGCTG 
AGCTTCCTCC AGGACAAGCT CAAGAGGCTA GAGAAGGAGC GGGCCATGGC CGAGGGCAAG 
ATCACCGTCA AGGAGGTGCT CAAGGTGGAG AAGGACGCGG CCACCGAGAG GGAGGTCAGC 
GATCTCACCC GCCAATATGA GGACGAGGCT GCCAAGGCTC GCGCTAGCCA GAGGGAGAAG 
ACGGAGCTGC TCCGAAAGAT ATGGGCCTTG GAGGAGGAGA ACGCCAAAGT GGTGGTGCAG 
GAGAAGGTGC GGGAGATCGT GCGGCCAGAC CCCAAGGCGG AAAGTGAAGT GGCGAACCTC 
CGCCTGGAGC TTGTGGAGCA GGAGCGAAAG TACCGGGGTG CCGAGGAGCA GCTCCGGAGC 
TACCAGAGTG AGCTGGAGGC CCTCAGGAGG CGAGGCCCCC AGGTGGAAGT CAAAGAGGTG 
ACTAAGGAAG TCATTAAGTA CAAGACTGAC CCTGAGATGG AGAAGGAGCT TCAGCGGCTC 
AGGGAGGAGA TCGTGGACAA GACCAGACTG ATCGAAAGGT GTGATTTAGA GATCTACCAG 
CTGAAAAAGG AAATCCAGGC CCTGAAAGAC ACCAAACCCC AGGTCCAGAC CAAAGAGGTG 
GTCCAGGAGA TCCTCCAATT CCAAGAAGAC CCTCAAACCA AGGAGGAGGT GGCGTCTCTG 
AGGGCAAAGC TCTCAGAGGA GCAGAAGAAA CAAGTGGATC TGGAGAGGGA AAGAGCTTCC 
CAGGAAGAGC AGATCGCCCG GAAAGAGGAG GAGCTCTCGC GGGTGAAGGA AAGGGTGGTG 
CAGCAGGAGG TGGTCAGGTA TGAGGAGGAG CCAGGCCTGC GGGCCGAGGC GAGCGCCTTT 
GCCGAGAGCA TCGATGTGGA GCTGCGGCAG ATTGACAAGC TGCGGGCAGA GCTGCGGCGG 
CTGCAGCGCC GGCGCACCGA GCTTGAGCGG CAGCTGGAGG AGCTAGAGCG CGAGCGGCAG 
GCCCGCAGGG AGGCCGAGCG CGAGGTACAG CGGTTGCAGC AGCGGCTGGC AGCGCTGGAG 
CAGGAAGAAG CTGAGGCCCG TGAGAAGGTA ACCCATACGC AGAAGGTGGT GCTGCAGCAG 
GACCCGCAGC AGGCGCGAGA GCATGCCCTG CTCCGACTCC AGCTGGAAGA AGAGCAGCAC 
CGGCGGCAGC TCCTGGAGGG GGAGCTCGAG ACCCTCCGGA GGAAACTGGC TGCACTGGAG 
AAGGCGGAGG TCAAGGAGAA GGTGGTGCTC TCCGAGAGTG TCCAGGTGGA GAAGGGCGAC 
ACCGAGCAAG AGATCCAGAG GCTCAAGAGC AGCCTGGAGG AGGAGAGCCG CAGCAAGCGC 
GAGCTGGACG TCGAGGTGAG CCGGCTGGAA GCCAGGCTTT CGGAGCTGGA ATTCCATAAC 
TCCAAGTCAT CCAAGGAACT AGACTTTCTG AGGGAAGAGA ACCACAAATT ACAGCTGGAG 
AGGCAAAACC TGCAGCTGGA GACCCGAAGG CTCCAATCGG AAATCAACAT GGCAGCGACG 
GAAACACGAG ACCTGCGGAA CATGACCGTG GCGGACTCTG GGACCAACCA TGACTCCAGA 
CTGTGGTCCC TGGAGAGGGA ACTGGATGAC CTCAAGAGGC TCTCCAAGGA CAAAGACCTC 
GAGATCGACG AGCTGCAGAA GCGCCTGGGC TCCGTGGCCG TCAAGCGGGA GCAGCGGGAG 
AACCACCTGC GGCGCTCCAT CGTAGTCATC CACCCTGACA CAGGCCGCGA GCTGTCCCCG 
GAGGAAGCCC ACCGTGCCGG GCTCATTGAC TGGAACATGT TCGTGAAACT CAGAAGCCAG 
GAGTGCGACT GGGAGGAGAT CTCAGTGAAG GGTCCCAATG GGGAGTCCTC AGTGATACAC 
GACAGGAAGT CTGGCAAGAA GTTCTCCATC GAAGAGGCCC TGCAGAGTGG CAGGCTGACC 
CCTGCTCAGT ATGACCGCTA TGTCAACAAG GATATGTCCA TCCAGGAGCT GGCGGTCTTG 
GTATCTGGGC AGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPL ( XM_017023374.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878863.1 Homo sapiens periplakin (PPL), transcript variant X1, mRNA.

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CloneID	OHu86120
Clone ID Related Accession (Same CDS sequence)	XM_017023374.1 , XM_017023374.2
Accession Version	XM_017023374.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	periplakin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017023374.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGGTA CGGGGGTCCA GGAACCGGGA ACTGAACCCG GAGTGCGGGA CTCTCGGGAA 
CGCGGGACCG AGGCCACCCT GAAGCTGGGA AGCGCCGCCA GCATCTCTAA CAAGGAGCTC 
TCGGAGCTGA TCGAGCAGCT GCAGAAGAAT GCCGACCAGG TGGAGAAGAA CATCGTGGAC 
ACAGAGGCCA AGATGCAGAG TGACCTGGCT CGGCTGCAGG AGGGTCGGCA GCCTGAGCAC 
CGGGACGTGA CCCTGCAGAA GGTGTTGGAC TCTGAGAAGC TGCTCTATGT GCTAGAGGCG 
GATGCGGCCA TTGCCAAGCA CATGAAGCAC CCACAGGGGG ACATGATCGC CGAGGATATC 
CGCCAGCTGA AGGAGCGTGT GACCAACCTG CGCGGGAAAC ACAAGCAGAT CTACAGGCTG 
GCGGTGAAGG AAGTGGATCC ACAGGTCAAC TGGGCGGCAC TGGTGGAGGA GAAGCTGGAC 
AAGCTGAACA ACCAGAGCTT TGGGACTGAC CTGCCGCTGG TGGACCACCA AGTGGAGGAG 
CATAACATCT TCCACAATGA GGTCAAGGCC ATCGGGCCCC ACCTGGCCAA GGACGGGGAC 
AAGGAGCAGA ACAGCGAACT CCGGGCCAAG TACCAGAAAC TGCTGGCAGC ATCACAGGCC 
CGGCAGCAGC ACCTGAGTTC GCTGCAGGAC TACATGCAGC GCTGCACCAA TGAGCTGTAC 
TGGCTGGACC AGCAGGCCAA GGGCCGCATG CAGTACGACT GGAGTGACCG CAACCTCGAC 
TACCCCAGCC GCCGGCGCCA GTATGAGAAT TTCATCAACC GGAACCTGGA GGCCAAAGAG 
GAGAGAATCA ACAAACTGCA CAGCGAGGGC GACCAGCTGC TGGCGGCCGA GCACCCCGGG 
AGGAACTCCA TTGAGGCGCA CATGGAGGCT GTGCACGCAG ACTGGAAGGA GTACCTGAAC 
CTGCTCATCT GCGAGGAGAG CCACCTCAAG TACATGGAGG ACTACCACCA GTTTCACGAA 
GACGTGAAGG ACGCTCAGGA GCTGCTGCGC AAGGTGGACT CGGACCTGAA CCAGAAGTAT 
GGCCCTGACT TCAAGGACCG GTACCAGATT GAGCTGCTGC TGCGGGAGCT GGATGACCAG 
GAGAAGGTGC TGGACAAGTA TGAGGACGTG GTGCAGGGGC TGCAGAAGCG AGGCCAGCAG 
GTGGTGCCCC TCAAGTACCG CCGGGAGACT CCGCTCAAGC CCATCCCCGT GGAGGCACTC 
TGTGACTTTG AGGGGGAGCA GGCCCTCAGG CCCTGCCGGG ATCCTGACTG TCCAGCCTGC 
CCCCGCCAGG GCCTGATCTC GCGGGGCTAC AGCTACACCC TGCAGAAGAA CAACGGGGAG 
AGCTGGGAGC TCATGGACAG CGCTGGGAAC AAGCTGATTG CTCCGGCCGT GTGTTTTGTG 
ATCCCCCCCA CAGACCCTGA GGCCCTGGCT CTGGCTGACA GCCTGGGCAG CCAGTACCGG 
AGCGTGCGGC AGAAGGCAGC TGGGAGCAAA CGCACGCTGC AGCAGCGGTA TGAGGTGCTG 
AAGACCGAGA ATCCCGGAGA TGCCTCTGAC CTACAGGGGC GGCAGCTGCT GGCTGGCTTG 
GACAAGGTGG CCAGCGACCT GGACCGGCAG GAGAAGGCCA TCACAGGGAT CCTGCGGCCA 
CCACTGGAGC AAGGCCGGGC TGTGCAGGAC AGTGCCGAGC GGGCCAAGGA CCTCAAGAAC 
ATCACCAACG AGCTACTGCG GATTGAACCT GAGAAGACGC GGAGCACGGC TGAGGGCGAA 
GCCTTCATCC AGGCCCTCCC AGGCAGTGGC ACCACACCCC TGCTGAGGAC CCGGGTGGAG 
GACACCAACC GGAAATACGA GCACCTCCTG CAGCTGCTGG ACTTGGCCCA GGAGAAGGTT 
GATGTGGCCA ACCGCCTGGA GAAGAGCCTG CAGCAGAGCT GGGAGTTGCT GGCCACACAC 
GAGAACCATC TGAATCAGGA TGACACAGTG CCTGAGAGCA GCCGTGTCCT GGACAGCAAG 
GGGCAGGAGC TGGCGGCCAT GGCCTGTGAG TTACAGGCCC AGAAGTCCCT CCTGGGTGAG 
GTGGAGCAGA ACTTGCAGGC GGCCAAGCAG TGCTCGAGCA CACTGGCCAG CCGCTTCCAG 
GAGCACTGTC CGGACCTGGA GCGCCAGGAG GCCGAGGTGC ACAAGCTGGG CCAGCGTTTC 
AACAACCTGC GCCAGCAGGT GGAACGCAGG GCGCAGAGCC TACAGAGCGC CAAGGCAGCC 
TACGAGCACT TCCACCGCGG CCATGACCAC GTGCTGCAGT TCCTAGTCAG CATCCCCAGT 
TACGAGCCCC AGGAGACAGA CAGCCTCAGC CAGATGGAGA CCAAGCTGAA GAACCAGAAG 
AACCTGCTAG ATGAGATAGC AAGTAGGGAG CAGGAAGTAC AGAAGATCTG TGCCAATTCC 
CAGCAGTACC AGCAAGCTGT AAAGGACTAT GAGTTAGAAG CAGAAAAACT AAGGTCTCTT 
CTCGACTTGG AGAATGGAAG GAGAAGCCAC GTGAGCAAGA GAGCCAGGCT CCAATCTCCT 
GCCACCAAAG TGAAGGAAGA GGAAGCAGCA CTTGCCGCCA AGTTCACTGA AGTTTATGCC 
ATCAACAGAC AGAGGCTGCA GAATCTGGAG TTTGCTCTGA ATCTCCTCAG ACAGCAGCCG 
GAAGTAGAAG TGACCCATGA GACCCTGCAA AGGAATAGGC CGGACTCTGG AGTGGAGGAG 
GCGTGGAAGA TCAGGAAGGA ACTGGATGAG GAGACTGAGC GGAGGCGGCA GCTGGAGAAC 
GAGGTCAAGA GCACCCAGGA AGAAATCTGG ACCTTGAGGA ATCAGGGGCC TCAGGAATCG 
GTGGTGAGGA AGGAGGTGCT CAAGAAGGTG CCGGATCCCG TGCTGGAGGA GAGCTTCCAG 
CAGCTGCAGC GGACGCTGGC AGAGGAGCAG CACAAGAACC AGCTGCTGCA GGAGGAGCTG 
GAGGCACTGC AGCTGCAGCT GCGTGCCCTG GAGCAGGAGA CCAGAGACGG GGGGCAGGAG 
TACGTGGTCA AGGAGGTCCT GCGCATCGAG CCTGACAGGG CCCAGGCGGA TGAGGTCTTG 
CAGCTGCGGG AGGAGCTGGA GGCACTGAGG CGGCAGAAGG GCGCCCGGGA GGCAGAGGTG 
CTCCTCCTGC AGCAGCGTGT GGCCGCCCTG GCTGAAGAGA AGAGCCGGGC GCAGGAGAAG 
GTCACAGAGA AAGAGGTGGT GAAACTGCAG AATGACCCCC AGCTGGAGGC AGAGTACCAG 
CAGCTGCAGG AGGACCACCA GCGCCAGGAC CAGCTCAGGG AGAAGCAGGA GGAGGAGCTG 
AGCTTCCTCC AGGACAAGCT CAAGAGGCTA GAGAAGGAGC GGGCCATGGC CGAGGGCAAG 
ATCACCGTCA AGGAGGTGCT CAAGGTGGAG AAGGACGCGG CCACCGAGAG GGAGGTCAGC 
GATCTCACCC GCCAATATGA GGACGAGGCT GCCAAGGCTC GCGCTAGCCA GAGGGAGAAG 
ACGGAGCTGC TCCGAAAGAT ATGGGCCTTG GAGGAGGAGA ACGCCAAAGT GGTGGTGCAG 
GAGAAGGTGC GGGAGATCGT GCGGCCAGAC CCCAAGGCGG AAAGTGAAGT GGCGAACCTC 
CGCCTGGAGC TTGTGGAGCA GGAGCGAAAG TACCGGGGTG CCGAGGAGCA GCTCCGGAGC 
TACCAGAGTG AGCTGGAGGC CCTCAGGAGG CGAGGCCCCC AGGTGGAAGT CAAAGAGGTG 
ACTAAGGAAG TCATTAAGTA CAAGACTGAC CCTGAGATGG AGAAGGAGCT TCAGCGGCTC 
AGGGAGGAGA TCGTGGACAA GACCAGACTG ATCGAAAGGT GTGATTTAGA GATCTACCAG 
CTGAAAAAGG AAATCCAGGC CCTGAAAGAC ACCAAACCCC AGGTCCAGAC CAAAGAGGTG 
GTCCAGGAGA TCCTCCAATT CCAAGAAGAC CCTCAAACCA AGGAGGAGGT GGCGTCTCTG 
AGGGCAAAGC TCTCAGAGGA GCAGAAGAAA CAAGTGGATC TGGAGAGGGA AAGAGCTTCC 
CAGGAAGAGC AGATCGCCCG GAAAGAGGAG GAGCTCTCGC GGGTGAAGGA AAGGGTGGTG 
CAGCAGGAGG TGGTCAGGTA TGAGGAGGAG CCAGGCCTGC GGGCCGAGGC GAGCGCCTTT 
GCCGAGAGCA TCGATGTGGA GCTGCGGCAG ATTGACAAGC TGCGGGCAGA GCTGCGGCGG 
CTGCAGCGCC GGCGCACCGA GCTTGAGCGG CAGCTGGAGG AGCTAGAGCG CGAGCGGCAG 
GCCCGCAGGG AGGCCGAGCG CGAGGTACAG CGGTTGCAGC AGCGGCTGGC AGCGCTGGAG 
CAGGAAGAAG CTGAGGCCCG TGAGAAGGTA ACCCATACGC AGAAGGTGGT GCTGCAGCAG 
GACCCGCAGC AGGCGCGAGA GCATGCCCTG CTCCGACTCC AGCTGGAAGA AGAGCAGCAC 
CGGCGGCAGC TCCTGGAGGG GGAGCTCGAG ACCCTCCGGA GGAAACTGGC TGCACTGGAG 
AAGGCGGAGG TCAAGGAGAA GGTGGTGCTC TCCGAGAGTG TCCAGGTGGA GAAGGGCGAC 
ACCGAGCAAG AGATCCAGAG GCTCAAGAGC AGCCTGGAGG AGGAGAGCCG CAGCAAGCGC 
GAGCTGGACG TCGAGGTGAG CCGGCTGGAA GCCAGGCTTT CGGAGCTGGA ATTCCATAAC 
TCCAAGTCAT CCAAGGAACT AGACTTTCTG AGGGAAGAGA ACCACAAATT ACAGCTGGAG 
AGGCAAAACC TGCAGCTGGA GACCCGAAGG CTCCAATCGG AAATCAACAT GGCAGCGACG 
GAAACACGAG ACCTGCGGAA CATGACCGTG GCGGACTCTG GGACCAACCA TGACTCCAGA 
CTGTGGTCCC TGGAGAGGGA ACTGGATGAC CTCAAGAGGC TCTCCAAGGA CAAAGACCTC 
GAGATCGACG AGCTGCAGAA GCGCCTGGGC TCCGTGGCCG TCAAGCGGGA GCAGCGGGAG 
AACCACCTGC GGCGCTCCAT CGTAGTCATC CACCCTGACA CAGGCCGCGA GCTGTCCCCG 
GAGGAAGCCC ACCGTGCCGG GCTCATTGAC TGGAACATGT TCGTGAAACT CAGAAGCCAG 
GAGTGCGACT GGGAGGAGAT CTCAGTGAAG GGTCCCAATG GGGAGTCCTC AGTGATACAC 
GACAGGAAGT CTGGCAAGAA GTTCTCCATC GAAGAGGCCC TGCAGAGTGG CAGGCTGACC 
CCTGCTCAGT ATGACCGCTA TGTCAACAAG GATATGTCCA TCCAGGAGCT GGCGGTCTTG 
GTATCTGGGC AGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878863.1
CDS	16..5373
Translation	MGGTGVQEPGTEPGVRDSRERGTEATLKLGSAASISNKELSELIEQLQKNADQVEKNIVDTEAKMQSDLARLQEGRQPEHRDVTLQKVLDSEKLLYVLEADAAIAKHMKHPQGDMIAEDIRQLKERVTNLRGKHKQIYRLAVKEVDPQVNWAALVEEKLDKLNNQSFGTDLPLVDHQVEEHNIFHNEVKAIGPHLAKDGDKEQNSELRAKYQKLLAASQARQQHLSSLQDYMQRCTNELYWLDQQAKGRMQYDWSDRNLDYPSRRRQYENFINRNLEAKEERINKLHSEGDQLLAAEHPGRNSIEAHMEAVHADWKEYLNLLICEESHLKYMEDYHQFHEDVKDAQELLRKVDSDLNQKYGPDFKDRYQIELLLRELDDQEKVLDKYEDVVQGLQKRGQQVVPLKYRRETPLKPIPVEALCDFEGEQALRPCRDPDCPACPRQGLISRGYSYTLQKNNGESWELMDSAGNKLIAPAVCFVIPPTDPEALALADSLGSQYRSVRQKAAGSKRTLQQRYEVLKTENPGDASDLQGRQLLAGLDKVASDLDRQEKAITGILRPPLEQGRAVQDSAERAKDLKNITNELLRIEPEKTRSTAEGEAFIQALPGSGTTPLLRTRVEDTNRKYEHLLQLLDLAQEKVDVANRLEKSLQQSWELLATHENHLNQDDTVPESSRVLDSKGQELAAMACELQAQKSLLGEVEQNLQAAKQCSSTLASRFQEHCPDLERQEAEVHKLGQRFNNLRQQVERRAQSLQSAKAAYEHFHRGHDHVLQFLVSIPSYEPQETDSLSQMETKLKNQKNLLDEIASREQEVQKICANSQQYQQAVKDYELEAEKLRSLLDLENGRRSHVSKRARLQSPATKVKEEEAALAAKFTEVYAINRQRLQNLEFALNLLRQQPEVEVTHETLQRNRPDSGVEEAWKIRKELDEETERRRQLENEVKSTQEEIWTLRNQGPQESVVRKEVLKKVPDPVLEESFQQLQRTLAEEQHKNQLLQEELEALQLQLRALEQETRDGGQEYVVKEVLRIEPDRAQADEVLQLREELEALRRQKGAREAEVLLLQQRVAALAEEKSRAQEKVTEKEVVKLQNDPQLEAEYQQLQEDHQRQDQLREKQEEELSFLQDKLKRLEKERAMAEGKITVKEVLKVEKDAATEREVSDLTRQYEDEAAKARASQREKTELLRKIWALEEENAKVVVQEKVREIVRPDPKAESEVANLRLELVEQERKYRGAEEQLRSYQSELEALRRRGPQVEVKEVTKEVIKYKTDPEMEKELQRLREEIVDKTRLIERCDLEIYQLKKEIQALKDTKPQVQTKEVVQEILQFQEDPQTKEEVASLRAKLSEEQKKQVDLERERASQEEQIARKEEELSRVKERVVQQEVVRYEEEPGLRAEASAFAESIDVELRQIDKLRAELRRLQRRRTELERQLEELERERQARREAEREVQRLQQRLAALEQEEAEAREKVTHTQKVVLQQDPQQAREHALLRLQLEEEQHRRQLLEGELETLRRKLAALEKAEVKEKVVLSESVQVEKGDTEQEIQRLKSSLEEESRSKRELDVEVSRLEARLSELEFHNSKSSKELDFLREENHKLQLERQNLQLETRRLQSEINMAATETRDLRNMTVADSGTNHDSRLWSLERELDDLKRLSKDKDLEIDELQKRLGSVAVKREQRENHLRRSIVVIHPDTGRELSPEEAHRAGLIDWNMFVKLRSQECDWEEISVKGPNGESSVIHDRKSGKKFSIEEALQSGRLTPAQYDRYVNKDMSIQELAVLVSGQK

Target ORF information:

RefSeq Version	XM_017023374.2
Organism	Homo sapiens(human)
Definition	Homo sapiens periplakin (PPL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023374.2

ORF Insert Sequence:

ATGGGGGGTA CGGGGGTCCA GGAACCGGGA ACTGAACCCG GAGTGCGGGA CTCTCGGGAA 
CGCGGGACCG AGGCCACCCT GAAGCTGGGA AGCGCCGCCA GCATCTCTAA CAAGGAGCTC 
TCGGAGCTGA TCGAGCAGCT GCAGAAGAAT GCCGACCAGG TGGAGAAGAA CATCGTGGAC 
ACAGAGGCCA AGATGCAGAG TGACCTGGCT CGGCTGCAGG AGGGTCGGCA GCCTGAGCAC 
CGGGACGTGA CCCTGCAGAA GGTGTTGGAC TCTGAGAAGC TGCTCTATGT GCTAGAGGCG 
GATGCGGCCA TTGCCAAGCA CATGAAGCAC CCACAGGGGG ACATGATCGC CGAGGATATC 
CGCCAGCTGA AGGAGCGTGT GACCAACCTG CGCGGGAAAC ACAAGCAGAT CTACAGGCTG 
GCGGTGAAGG AAGTGGATCC ACAGGTCAAC TGGGCGGCAC TGGTGGAGGA GAAGCTGGAC 
AAGCTGAACA ACCAGAGCTT TGGGACTGAC CTGCCGCTGG TGGACCACCA AGTGGAGGAG 
CATAACATCT TCCACAATGA GGTCAAGGCC ATCGGGCCCC ACCTGGCCAA GGACGGGGAC 
AAGGAGCAGA ACAGCGAACT CCGGGCCAAG TACCAGAAAC TGCTGGCAGC ATCACAGGCC 
CGGCAGCAGC ACCTGAGTTC GCTGCAGGAC TACATGCAGC GCTGCACCAA TGAGCTGTAC 
TGGCTGGACC AGCAGGCCAA GGGCCGCATG CAGTACGACT GGAGTGACCG CAACCTCGAC 
TACCCCAGCC GCCGGCGCCA GTATGAGAAT TTCATCAACC GGAACCTGGA GGCCAAAGAG 
GAGAGAATCA ACAAACTGCA CAGCGAGGGC GACCAGCTGC TGGCGGCCGA GCACCCCGGG 
AGGAACTCCA TTGAGGCGCA CATGGAGGCT GTGCACGCAG ACTGGAAGGA GTACCTGAAC 
CTGCTCATCT GCGAGGAGAG CCACCTCAAG TACATGGAGG ACTACCACCA GTTTCACGAA 
GACGTGAAGG ACGCTCAGGA GCTGCTGCGC AAGGTGGACT CGGACCTGAA CCAGAAGTAT 
GGCCCTGACT TCAAGGACCG GTACCAGATT GAGCTGCTGC TGCGGGAGCT GGATGACCAG 
GAGAAGGTGC TGGACAAGTA TGAGGACGTG GTGCAGGGGC TGCAGAAGCG AGGCCAGCAG 
GTGGTGCCCC TCAAGTACCG CCGGGAGACT CCGCTCAAGC CCATCCCCGT GGAGGCACTC 
TGTGACTTTG AGGGGGAGCA GGCCCTCAGG CCCTGCCGGG ATCCTGACTG TCCAGCCTGC 
CCCCGCCAGG GCCTGATCTC GCGGGGCTAC AGCTACACCC TGCAGAAGAA CAACGGGGAG 
AGCTGGGAGC TCATGGACAG CGCTGGGAAC AAGCTGATTG CTCCGGCCGT GTGTTTTGTG 
ATCCCCCCCA CAGACCCTGA GGCCCTGGCT CTGGCTGACA GCCTGGGCAG CCAGTACCGG 
AGCGTGCGGC AGAAGGCAGC TGGGAGCAAA CGCACGCTGC AGCAGCGGTA TGAGGTGCTG 
AAGACCGAGA ATCCCGGAGA TGCCTCTGAC CTACAGGGGC GGCAGCTGCT GGCTGGCTTG 
GACAAGGTGG CCAGCGACCT GGACCGGCAG GAGAAGGCCA TCACAGGGAT CCTGCGGCCA 
CCACTGGAGC AAGGCCGGGC TGTGCAGGAC AGTGCCGAGC GGGCCAAGGA CCTCAAGAAC 
ATCACCAACG AGCTACTGCG GATTGAACCT GAGAAGACGC GGAGCACGGC TGAGGGCGAA 
GCCTTCATCC AGGCCCTCCC AGGCAGTGGC ACCACACCCC TGCTGAGGAC CCGGGTGGAG 
GACACCAACC GGAAATACGA GCACCTCCTG CAGCTGCTGG ACTTGGCCCA GGAGAAGGTT 
GATGTGGCCA ACCGCCTGGA GAAGAGCCTG CAGCAGAGCT GGGAGTTGCT GGCCACACAC 
GAGAACCATC TGAATCAGGA TGACACAGTG CCTGAGAGCA GCCGTGTCCT GGACAGCAAG 
GGGCAGGAGC TGGCGGCCAT GGCCTGTGAG TTACAGGCCC AGAAGTCCCT CCTGGGTGAG 
GTGGAGCAGA ACTTGCAGGC GGCCAAGCAG TGCTCGAGCA CACTGGCCAG CCGCTTCCAG 
GAGCACTGTC CGGACCTGGA GCGCCAGGAG GCCGAGGTGC ACAAGCTGGG CCAGCGTTTC 
AACAACCTGC GCCAGCAGGT GGAACGCAGG GCGCAGAGCC TACAGAGCGC CAAGGCAGCC 
TACGAGCACT TCCACCGCGG CCATGACCAC GTGCTGCAGT TCCTAGTCAG CATCCCCAGT 
TACGAGCCCC AGGAGACAGA CAGCCTCAGC CAGATGGAGA CCAAGCTGAA GAACCAGAAG 
AACCTGCTAG ATGAGATAGC AAGTAGGGAG CAGGAAGTAC AGAAGATCTG TGCCAATTCC 
CAGCAGTACC AGCAAGCTGT AAAGGACTAT GAGTTAGAAG CAGAAAAACT AAGGTCTCTT 
CTCGACTTGG AGAATGGAAG GAGAAGCCAC GTGAGCAAGA GAGCCAGGCT CCAATCTCCT 
GCCACCAAAG TGAAGGAAGA GGAAGCAGCA CTTGCCGCCA AGTTCACTGA AGTTTATGCC 
ATCAACAGAC AGAGGCTGCA GAATCTGGAG TTTGCTCTGA ATCTCCTCAG ACAGCAGCCG 
GAAGTAGAAG TGACCCATGA GACCCTGCAA AGGAATAGGC CGGACTCTGG AGTGGAGGAG 
GCGTGGAAGA TCAGGAAGGA ACTGGATGAG GAGACTGAGC GGAGGCGGCA GCTGGAGAAC 
GAGGTCAAGA GCACCCAGGA AGAAATCTGG ACCTTGAGGA ATCAGGGGCC TCAGGAATCG 
GTGGTGAGGA AGGAGGTGCT CAAGAAGGTG CCGGATCCCG TGCTGGAGGA GAGCTTCCAG 
CAGCTGCAGC GGACGCTGGC AGAGGAGCAG CACAAGAACC AGCTGCTGCA GGAGGAGCTG 
GAGGCACTGC AGCTGCAGCT GCGTGCCCTG GAGCAGGAGA CCAGAGACGG GGGGCAGGAG 
TACGTGGTCA AGGAGGTCCT GCGCATCGAG CCTGACAGGG CCCAGGCGGA TGAGGTCTTG 
CAGCTGCGGG AGGAGCTGGA GGCACTGAGG CGGCAGAAGG GCGCCCGGGA GGCAGAGGTG 
CTCCTCCTGC AGCAGCGTGT GGCCGCCCTG GCTGAAGAGA AGAGCCGGGC GCAGGAGAAG 
GTCACAGAGA AAGAGGTGGT GAAACTGCAG AATGACCCCC AGCTGGAGGC AGAGTACCAG 
CAGCTGCAGG AGGACCACCA GCGCCAGGAC CAGCTCAGGG AGAAGCAGGA GGAGGAGCTG 
AGCTTCCTCC AGGACAAGCT CAAGAGGCTA GAGAAGGAGC GGGCCATGGC CGAGGGCAAG 
ATCACCGTCA AGGAGGTGCT CAAGGTGGAG AAGGACGCGG CCACCGAGAG GGAGGTCAGC 
GATCTCACCC GCCAATATGA GGACGAGGCT GCCAAGGCTC GCGCTAGCCA GAGGGAGAAG 
ACGGAGCTGC TCCGAAAGAT ATGGGCCTTG GAGGAGGAGA ACGCCAAAGT GGTGGTGCAG 
GAGAAGGTGC GGGAGATCGT GCGGCCAGAC CCCAAGGCGG AAAGTGAAGT GGCGAACCTC 
CGCCTGGAGC TTGTGGAGCA GGAGCGAAAG TACCGGGGTG CCGAGGAGCA GCTCCGGAGC 
TACCAGAGTG AGCTGGAGGC CCTCAGGAGG CGAGGCCCCC AGGTGGAAGT CAAAGAGGTG 
ACTAAGGAAG TCATTAAGTA CAAGACTGAC CCTGAGATGG AGAAGGAGCT TCAGCGGCTC 
AGGGAGGAGA TCGTGGACAA GACCAGACTG ATCGAAAGGT GTGATTTAGA GATCTACCAG 
CTGAAAAAGG AAATCCAGGC CCTGAAAGAC ACCAAACCCC AGGTCCAGAC CAAAGAGGTG 
GTCCAGGAGA TCCTCCAATT CCAAGAAGAC CCTCAAACCA AGGAGGAGGT GGCGTCTCTG 
AGGGCAAAGC TCTCAGAGGA GCAGAAGAAA CAAGTGGATC TGGAGAGGGA AAGAGCTTCC 
CAGGAAGAGC AGATCGCCCG GAAAGAGGAG GAGCTCTCGC GGGTGAAGGA AAGGGTGGTG 
CAGCAGGAGG TGGTCAGGTA TGAGGAGGAG CCAGGCCTGC GGGCCGAGGC GAGCGCCTTT 
GCCGAGAGCA TCGATGTGGA GCTGCGGCAG ATTGACAAGC TGCGGGCAGA GCTGCGGCGG 
CTGCAGCGCC GGCGCACCGA GCTTGAGCGG CAGCTGGAGG AGCTAGAGCG CGAGCGGCAG 
GCCCGCAGGG AGGCCGAGCG CGAGGTACAG CGGTTGCAGC AGCGGCTGGC AGCGCTGGAG 
CAGGAAGAAG CTGAGGCCCG TGAGAAGGTA ACCCATACGC AGAAGGTGGT GCTGCAGCAG 
GACCCGCAGC AGGCGCGAGA GCATGCCCTG CTCCGACTCC AGCTGGAAGA AGAGCAGCAC 
CGGCGGCAGC TCCTGGAGGG GGAGCTCGAG ACCCTCCGGA GGAAACTGGC TGCACTGGAG 
AAGGCGGAGG TCAAGGAGAA GGTGGTGCTC TCCGAGAGTG TCCAGGTGGA GAAGGGCGAC 
ACCGAGCAAG AGATCCAGAG GCTCAAGAGC AGCCTGGAGG AGGAGAGCCG CAGCAAGCGC 
GAGCTGGACG TCGAGGTGAG CCGGCTGGAA GCCAGGCTTT CGGAGCTGGA ATTCCATAAC 
TCCAAGTCAT CCAAGGAACT AGACTTTCTG AGGGAAGAGA ACCACAAATT ACAGCTGGAG 
AGGCAAAACC TGCAGCTGGA GACCCGAAGG CTCCAATCGG AAATCAACAT GGCAGCGACG 
GAAACACGAG ACCTGCGGAA CATGACCGTG GCGGACTCTG GGACCAACCA TGACTCCAGA 
CTGTGGTCCC TGGAGAGGGA ACTGGATGAC CTCAAGAGGC TCTCCAAGGA CAAAGACCTC 
GAGATCGACG AGCTGCAGAA GCGCCTGGGC TCCGTGGCCG TCAAGCGGGA GCAGCGGGAG 
AACCACCTGC GGCGCTCCAT CGTAGTCATC CACCCTGACA CAGGCCGCGA GCTGTCCCCG 
GAGGAAGCCC ACCGTGCCGG GCTCATTGAC TGGAACATGT TCGTGAAACT CAGAAGCCAG 
GAGTGCGACT GGGAGGAGAT CTCAGTGAAG GGTCCCAATG GGGAGTCCTC AGTGATACAC 
GACAGGAAGT CTGGCAAGAA GTTCTCCATC GAAGAGGCCC TGCAGAGTGG CAGGCTGACC 
CCTGCTCAGT ATGACCGCTA TGTCAACAAG GATATGTCCA TCCAGGAGCT GGCGGTCTTG 
GTATCTGGGC AGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPL ( XM_017023375.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878864.1 Homo sapiens periplakin (PPL), transcript variant X2, mRNA.

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CloneID	OHu86121
Clone ID Related Accession (Same CDS sequence)	XM_017023375.1 , XM_017023375.2
Accession Version	XM_017023375.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	periplakin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCCACTGT GCAGACCCGG 
AGCATCTCTA ACAAGGAGCT CTCGGAGCTG ATCGAGCAGC TGCAGAAGAA TGCCGACCAG 
GTGGAGAAGA ACATCGTGGA CACAGAGGCC AAGATGCAGA GTGACCTGGC TCGGCTGCAG 
GAGGGTCGGC AGCCTGAGCA CCGGGACGTG ACCCTGCAGA AGGTGTTGGA CTCTGAGAAG 
CTGCTCTATG TGCTAGAGGC GGATGCGGCC ATTGCCAAGC ACATGAAGCA CCCACAGGGG 
GACATGATCG CCGAGGATAT CCGCCAGCTG AAGGAGCGTG TGACCAACCT GCGCGGGAAA 
CACAAGCAGA TCTACAGGCT GGCGGTGAAG GAAGTGGATC CACAGGTCAA CTGGGCGGCA 
CTGGTGGAGG AGAAGCTGGA CAAGCTGAAC AACCAGAGCT TTGGGACTGA CCTGCCGCTG 
GTGGACCACC AAGTGGAGGA GCATAACATC TTCCACAATG AGGTCAAGGC CATCGGGCCC 
CACCTGGCCA AGGACGGGGA CAAGGAGCAG AACAGCGAAC TCCGGGCCAA GTACCAGAAA 
CTGCTGGCAG CATCACAGGC CCGGCAGCAG CACCTGAGTT CGCTGCAGGA CTACATGCAG 
CGCTGCACCA ATGAGCTGTA CTGGCTGGAC CAGCAGGCCA AGGGCCGCAT GCAGTACGAC 
TGGAGTGACC GCAACCTCGA CTACCCCAGC CGCCGGCGCC AGTATGAGAA TTTCATCAAC 
CGGAACCTGG AGGCCAAAGA GGAGAGAATC AACAAACTGC ACAGCGAGGG CGACCAGCTG 
CTGGCGGCCG AGCACCCCGG GAGGAACTCC ATTGAGGCGC ACATGGAGGC TGTGCACGCA 
GACTGGAAGG AGTACCTGAA CCTGCTCATC TGCGAGGAGA GCCACCTCAA GTACATGGAG 
GACTACCACC AGTTTCACGA AGACGTGAAG GACGCTCAGG AGCTGCTGCG CAAGGTGGAC 
TCGGACCTGA ACCAGAAGTA TGGCCCTGAC TTCAAGGACC GGTACCAGAT TGAGCTGCTG 
CTGCGGGAGC TGGATGACCA GGAGAAGGTG CTGGACAAGT ATGAGGACGT GGTGCAGGGG 
CTGCAGAAGC GAGGCCAGCA GGTGGTGCCC CTCAAGTACC GCCGGGAGAC TCCGCTCAAG 
CCCATCCCCG TGGAGGCACT CTGTGACTTT GAGGGGGAGC AGGCCCTCAG GCCCTGCCGG 
GATCCTGACT GTCCAGCCTG CCCCCGCCAG GGCCTGATCT CGCGGGGCTA CAGCTACACC 
CTGCAGAAGA ACAACGGGGA GAGCTGGGAG CTCATGGACA GCGCTGGGAA CAAGCTGATT 
GCTCCGGCCG TGTGTTTTGT GATCCCCCCC ACAGACCCTG AGGCCCTGGC TCTGGCTGAC 
AGCCTGGGCA GCCAGTACCG GAGCGTGCGG CAGAAGGCAG CTGGGAGCAA ACGCACGCTG 
CAGCAGCGGT ATGAGGTGCT GAAGACCGAG AATCCCGGAG ATGCCTCTGA CCTACAGGGG 
CGGCAGCTGC TGGCTGGCTT GGACAAGGTG GCCAGCGACC TGGACCGGCA GGAGAAGGCC 
ATCACAGGGA TCCTGCGGCC ACCACTGGAG CAAGGCCGGG CTGTGCAGGA CAGTGCCGAG 
CGGGCCAAGG ACCTCAAGAA CATCACCAAC GAGCTACTGC GGATTGAACC TGAGAAGACG 
CGGAGCACGG CTGAGGGCGA AGCCTTCATC CAGGCCCTCC CAGGCAGTGG CACCACACCC 
CTGCTGAGGA CCCGGGTGGA GGACACCAAC CGGAAATACG AGCACCTCCT GCAGCTGCTG 
GACTTGGCCC AGGAGAAGGT TGATGTGGCC AACCGCCTGG AGAAGAGCCT GCAGCAGAGC 
TGGGAGTTGC TGGCCACACA CGAGAACCAT CTGAATCAGG ATGACACAGT GCCTGAGAGC 
AGCCGTGTCC TGGACAGCAA GGGGCAGGAG CTGGCGGCCA TGGCCTGTGA GTTACAGGCC 
CAGAAGTCCC TCCTGGGTGA GGTGGAGCAG AACTTGCAGG CGGCCAAGCA GTGCTCGAGC 
ACACTGGCCA GCCGCTTCCA GGAGCACTGT CCGGACCTGG AGCGCCAGGA GGCCGAGGTG 
CACAAGCTGG GCCAGCGTTT CAACAACCTG CGCCAGCAGG TGGAACGCAG GGCGCAGAGC 
CTACAGAGCG CCAAGGCAGC CTACGAGCAC TTCCACCGCG GCCATGACCA CGTGCTGCAG 
TTCCTAGTCA GCATCCCCAG TTACGAGCCC CAGGAGACAG ACAGCCTCAG CCAGATGGAG 
ACCAAGCTGA AGAACCAGAA GAACCTGCTA GATGAGATAG CAAGTAGGGA GCAGGAAGTA 
CAGAAGATCT GTGCCAATTC CCAGCAGTAC CAGCAAGCTG TAAAGGACTA TGAGTTAGAA 
GCAGAAAAAC TAAGGTCTCT TCTCGACTTG GAGAATGGAA GGAGAAGCCA CGTGAGCAAG 
AGAGCCAGGC TCCAATCTCC TGCCACCAAA GTGAAGGAAG AGGAAGCAGC ACTTGCCGCC 
AAGTTCACTG AAGTTTATGC CATCAACAGA CAGAGGCTGC AGAATCTGGA GTTTGCTCTG 
AATCTCCTCA GACAGCAGCC GGAAGTAGAA GTGACCCATG AGACCCTGCA AAGGAATAGG 
CCGGACTCTG GAGTGGAGGA GGCGTGGAAG ATCAGGAAGG AACTGGATGA GGAGACTGAG 
CGGAGGCGGC AGCTGGAGAA CGAGGTCAAG AGCACCCAGG AAGAAATCTG GACCTTGAGG 
AATCAGGGGC CTCAGGAATC GGTGGTGAGG AAGGAGGTGC TCAAGAAGGT GCCGGATCCC 
GTGCTGGAGG AGAGCTTCCA GCAGCTGCAG CGGACGCTGG CAGAGGAGCA GCACAAGAAC 
CAGCTGCTGC AGGAGGAGCT GGAGGCACTG CAGCTGCAGC TGCGTGCCCT GGAGCAGGAG 
ACCAGAGACG GGGGGCAGGA GTACGTGGTC AAGGAGGTCC TGCGCATCGA GCCTGACAGG 
GCCCAGGCGG ATGAGGTCTT GCAGCTGCGG GAGGAGCTGG AGGCACTGAG GCGGCAGAAG 
GGCGCCCGGG AGGCAGAGGT GCTCCTCCTG CAGCAGCGTG TGGCCGCCCT GGCTGAAGAG 
AAGAGCCGGG CGCAGGAGAA GGTCACAGAG AAAGAGGTGG TGAAACTGCA GAATGACCCC 
CAGCTGGAGG CAGAGTACCA GCAGCTGCAG GAGGACCACC AGCGCCAGGA CCAGCTCAGG 
GAGAAGCAGG AGGAGGAGCT GAGCTTCCTC CAGGACAAGC TCAAGAGGCT AGAGAAGGAG 
CGGGCCATGG CCGAGGGCAA GATCACCGTC AAGGAGGTGC TCAAGGTGGA GAAGGACGCG 
GCCACCGAGA GGGAGGTCAG CGATCTCACC CGCCAATATG AGGACGAGGC TGCCAAGGCT 
CGCGCTAGCC AGAGGGAGAA GACGGAGCTG CTCCGAAAGA TATGGGCCTT GGAGGAGGAG 
AACGCCAAAG TGGTGGTGCA GGAGAAGGTG CGGGAGATCG TGCGGCCAGA CCCCAAGGCG 
GAAAGTGAAG TGGCGAACCT CCGCCTGGAG CTTGTGGAGC AGGAGCGAAA GTACCGGGGT 
GCCGAGGAGC AGCTCCGGAG CTACCAGAGT GAGCTGGAGG CCCTCAGGAG GCGAGGCCCC 
CAGGTGGAAG TCAAAGAGGT GACTAAGGAA GTCATTAAGT ACAAGACTGA CCCTGAGATG 
GAGAAGGAGC TTCAGCGGCT CAGGGAGGAG ATCGTGGACA AGACCAGACT GATCGAAAGG 
TGTGATTTAG AGATCTACCA GCTGAAAAAG GAAATCCAGG CCCTGAAAGA CACCAAACCC 
CAGGTCCAGA CCAAAGAGGT GGTCCAGGAG ATCCTCCAAT TCCAAGAAGA CCCTCAAACC 
AAGGAGGAGG TGGCGTCTCT GAGGGCAAAG CTCTCAGAGG AGCAGAAGAA ACAAGTGGAT 
CTGGAGAGGG AAAGAGCTTC CCAGGAAGAG CAGATCGCCC GGAAAGAGGA GGAGCTCTCG 
CGGGTGAAGG AAAGGGTGGT GCAGCAGGAG GTGGTCAGGT ATGAGGAGGA GCCAGGCCTG 
CGGGCCGAGG CGAGCGCCTT TGCCGAGAGC ATCGATGTGG AGCTGCGGCA GATTGACAAG 
CTGCGGGCAG AGCTGCGGCG GCTGCAGCGC CGGCGCACCG AGCTTGAGCG GCAGCTGGAG 
GAGCTAGAGC GCGAGCGGCA GGCCCGCAGG GAGGCCGAGC GCGAGGTACA GCGGTTGCAG 
CAGCGGCTGG CAGCGCTGGA GCAGGAAGAA GCTGAGGCCC GTGAGAAGGT AACCCATACG 
CAGAAGGTGG TGCTGCAGCA GGACCCGCAG CAGGCGCGAG AGCATGCCCT GCTCCGACTC 
CAGCTGGAAG AAGAGCAGCA CCGGCGGCAG CTCCTGGAGG GGGAGCTCGA GACCCTCCGG 
AGGAAACTGG CTGCACTGGA GAAGGCGGAG GTCAAGGAGA AGGTGGTGCT CTCCGAGAGT 
GTCCAGGTGG AGAAGGGCGA CACCGAGCAA GAGATCCAGA GGCTCAAGAG CAGCCTGGAG 
GAGGAGAGCC GCAGCAAGCG CGAGCTGGAC GTCGAGGTGA GCCGGCTGGA AGCCAGGCTT 
TCGGAGCTGG AATTCCATAA CTCCAAGTCA TCCAAGGAAC TAGACTTTCT GAGGGAAGAG 
AACCACAAAT TACAGCTGGA GAGGCAAAAC CTGCAGCTGG AGACCCGAAG GCTCCAATCG 
GAAATCAACA TGGCAGCGAC GGAAACACGA GACCTGCGGA ACATGACCGT GGCGGACTCT 
GGGACCAACC ATGACTCCAG ACTGTGGTCC CTGGAGAGGG AACTGGATGA CCTCAAGAGG 
CTCTCCAAGG ACAAAGACCT CGAGATCGAC GAGCTGCAGA AGCGCCTGGG CTCCGTGGCC 
GTCAAGCGGG AGCAGCGGGA GAACCACCTG CGGCGCTCCA TCGTAGTCAT CCACCCTGAC 
ACAGGCCGCG AGCTGTCCCC GGAGGAAGCC CACCGTGCCG GGCTCATTGA CTGGAACATG 
TTCGTGAAAC TCAGAAGCCA GGAGTGCGAC TGGGAGGAGA TCTCAGTGAA GGGTCCCAAT 
GGGGAGTCCT CAGTGATACA CGACAGGAAG TCTGGCAAGA AGTTCTCCAT CGAAGAGGCC 
CTGCAGAGTG GCAGGCTGAC CCCTGCTCAG TATGACCGCT ATGTCAACAA GGATATGTCC 
ATCCAGGAGC TGGCGGTCTT GGTATCTGGG CAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878864.1
CDS	67..5385
Translation	MNSLFRKRNKGKYSPTVQTRSISNKELSELIEQLQKNADQVEKNIVDTEAKMQSDLARLQEGRQPEHRDVTLQKVLDSEKLLYVLEADAAIAKHMKHPQGDMIAEDIRQLKERVTNLRGKHKQIYRLAVKEVDPQVNWAALVEEKLDKLNNQSFGTDLPLVDHQVEEHNIFHNEVKAIGPHLAKDGDKEQNSELRAKYQKLLAASQARQQHLSSLQDYMQRCTNELYWLDQQAKGRMQYDWSDRNLDYPSRRRQYENFINRNLEAKEERINKLHSEGDQLLAAEHPGRNSIEAHMEAVHADWKEYLNLLICEESHLKYMEDYHQFHEDVKDAQELLRKVDSDLNQKYGPDFKDRYQIELLLRELDDQEKVLDKYEDVVQGLQKRGQQVVPLKYRRETPLKPIPVEALCDFEGEQALRPCRDPDCPACPRQGLISRGYSYTLQKNNGESWELMDSAGNKLIAPAVCFVIPPTDPEALALADSLGSQYRSVRQKAAGSKRTLQQRYEVLKTENPGDASDLQGRQLLAGLDKVASDLDRQEKAITGILRPPLEQGRAVQDSAERAKDLKNITNELLRIEPEKTRSTAEGEAFIQALPGSGTTPLLRTRVEDTNRKYEHLLQLLDLAQEKVDVANRLEKSLQQSWELLATHENHLNQDDTVPESSRVLDSKGQELAAMACELQAQKSLLGEVEQNLQAAKQCSSTLASRFQEHCPDLERQEAEVHKLGQRFNNLRQQVERRAQSLQSAKAAYEHFHRGHDHVLQFLVSIPSYEPQETDSLSQMETKLKNQKNLLDEIASREQEVQKICANSQQYQQAVKDYELEAEKLRSLLDLENGRRSHVSKRARLQSPATKVKEEEAALAAKFTEVYAINRQRLQNLEFALNLLRQQPEVEVTHETLQRNRPDSGVEEAWKIRKELDEETERRRQLENEVKSTQEEIWTLRNQGPQESVVRKEVLKKVPDPVLEESFQQLQRTLAEEQHKNQLLQEELEALQLQLRALEQETRDGGQEYVVKEVLRIEPDRAQADEVLQLREELEALRRQKGAREAEVLLLQQRVAALAEEKSRAQEKVTEKEVVKLQNDPQLEAEYQQLQEDHQRQDQLREKQEEELSFLQDKLKRLEKERAMAEGKITVKEVLKVEKDAATEREVSDLTRQYEDEAAKARASQREKTELLRKIWALEEENAKVVVQEKVREIVRPDPKAESEVANLRLELVEQERKYRGAEEQLRSYQSELEALRRRGPQVEVKEVTKEVIKYKTDPEMEKELQRLREEIVDKTRLIERCDLEIYQLKKEIQALKDTKPQVQTKEVVQEILQFQEDPQTKEEVASLRAKLSEEQKKQVDLERERASQEEQIARKEEELSRVKERVVQQEVVRYEEEPGLRAEASAFAESIDVELRQIDKLRAELRRLQRRRTELERQLEELERERQARREAEREVQRLQQRLAALEQEEAEAREKVTHTQKVVLQQDPQQAREHALLRLQLEEEQHRRQLLEGELETLRRKLAALEKAEVKEKVVLSESVQVEKGDTEQEIQRLKSSLEEESRSKRELDVEVSRLEARLSELEFHNSKSSKELDFLREENHKLQLERQNLQLETRRLQSEINMAATETRDLRNMTVADSGTNHDSRLWSLERELDDLKRLSKDKDLEIDELQKRLGSVAVKREQRENHLRRSIVVIHPDTGRELSPEEAHRAGLIDWNMFVKLRSQECDWEEISVKGPNGESSVIHDRKSGKKFSIEEALQSGRLTPAQYDRYVNKDMSIQELAVLVSGQK

Target ORF information:

RefSeq Version	XM_017023375.1
Organism	Homo sapiens(human)
Definition	Homo sapiens periplakin (PPL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023375.1

ORF Insert Sequence:

ATGAACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCCACTGT GCAGACCCGG 
AGCATCTCTA ACAAGGAGCT CTCGGAGCTG ATCGAGCAGC TGCAGAAGAA TGCCGACCAG 
GTGGAGAAGA ACATCGTGGA CACAGAGGCC AAGATGCAGA GTGACCTGGC TCGGCTGCAG 
GAGGGTCGGC AGCCTGAGCA CCGGGACGTG ACCCTGCAGA AGGTGTTGGA CTCTGAGAAG 
CTGCTCTATG TGCTAGAGGC GGATGCGGCC ATTGCCAAGC ACATGAAGCA CCCACAGGGG 
GACATGATCG CCGAGGATAT CCGCCAGCTG AAGGAGCGTG TGACCAACCT GCGCGGGAAA 
CACAAGCAGA TCTACAGGCT GGCGGTGAAG GAAGTGGATC CACAGGTCAA CTGGGCGGCA 
CTGGTGGAGG AGAAGCTGGA CAAGCTGAAC AACCAGAGCT TTGGGACTGA CCTGCCGCTG 
GTGGACCACC AAGTGGAGGA GCATAACATC TTCCACAATG AGGTCAAGGC CATCGGGCCC 
CACCTGGCCA AGGACGGGGA CAAGGAGCAG AACAGCGAAC TCCGGGCCAA GTACCAGAAA 
CTGCTGGCAG CATCACAGGC CCGGCAGCAG CACCTGAGTT CGCTGCAGGA CTACATGCAG 
CGCTGCACCA ATGAGCTGTA CTGGCTGGAC CAGCAGGCCA AGGGCCGCAT GCAGTACGAC 
TGGAGTGACC GCAACCTCGA CTACCCCAGC CGCCGGCGCC AGTATGAGAA TTTCATCAAC 
CGGAACCTGG AGGCCAAAGA GGAGAGAATC AACAAACTGC ACAGCGAGGG CGACCAGCTG 
CTGGCGGCCG AGCACCCCGG GAGGAACTCC ATTGAGGCGC ACATGGAGGC TGTGCACGCA 
GACTGGAAGG AGTACCTGAA CCTGCTCATC TGCGAGGAGA GCCACCTCAA GTACATGGAG 
GACTACCACC AGTTTCACGA AGACGTGAAG GACGCTCAGG AGCTGCTGCG CAAGGTGGAC 
TCGGACCTGA ACCAGAAGTA TGGCCCTGAC TTCAAGGACC GGTACCAGAT TGAGCTGCTG 
CTGCGGGAGC TGGATGACCA GGAGAAGGTG CTGGACAAGT ATGAGGACGT GGTGCAGGGG 
CTGCAGAAGC GAGGCCAGCA GGTGGTGCCC CTCAAGTACC GCCGGGAGAC TCCGCTCAAG 
CCCATCCCCG TGGAGGCACT CTGTGACTTT GAGGGGGAGC AGGCCCTCAG GCCCTGCCGG 
GATCCTGACT GTCCAGCCTG CCCCCGCCAG GGCCTGATCT CGCGGGGCTA CAGCTACACC 
CTGCAGAAGA ACAACGGGGA GAGCTGGGAG CTCATGGACA GCGCTGGGAA CAAGCTGATT 
GCTCCGGCCG TGTGTTTTGT GATCCCCCCC ACAGACCCTG AGGCCCTGGC TCTGGCTGAC 
AGCCTGGGCA GCCAGTACCG GAGCGTGCGG CAGAAGGCAG CTGGGAGCAA ACGCACGCTG 
CAGCAGCGGT ATGAGGTGCT GAAGACCGAG AATCCCGGAG ATGCCTCTGA CCTACAGGGG 
CGGCAGCTGC TGGCTGGCTT GGACAAGGTG GCCAGCGACC TGGACCGGCA GGAGAAGGCC 
ATCACAGGGA TCCTGCGGCC ACCACTGGAG CAAGGCCGGG CTGTGCAGGA CAGTGCCGAG 
CGGGCCAAGG ACCTCAAGAA CATCACCAAC GAGCTACTGC GGATTGAACC TGAGAAGACG 
CGGAGCACGG CTGAGGGCGA AGCCTTCATC CAGGCCCTCC CAGGCAGTGG CACCACACCC 
CTGCTGAGGA CCCGGGTGGA GGACACCAAC CGGAAATACG AGCACCTCCT GCAGCTGCTG 
GACTTGGCCC AGGAGAAGGT TGATGTGGCC AACCGCCTGG AGAAGAGCCT GCAGCAGAGC 
TGGGAGTTGC TGGCCACACA CGAGAACCAT CTGAATCAGG ATGACACAGT GCCTGAGAGC 
AGCCGTGTCC TGGACAGCAA GGGGCAGGAG CTGGCGGCCA TGGCCTGTGA GTTACAGGCC 
CAGAAGTCCC TCCTGGGTGA GGTGGAGCAG AACTTGCAGG CGGCCAAGCA GTGCTCGAGC 
ACACTGGCCA GCCGCTTCCA GGAGCACTGT CCGGACCTGG AGCGCCAGGA GGCCGAGGTG 
CACAAGCTGG GCCAGCGTTT CAACAACCTG CGCCAGCAGG TGGAACGCAG GGCGCAGAGC 
CTACAGAGCG CCAAGGCAGC CTACGAGCAC TTCCACCGCG GCCATGACCA CGTGCTGCAG 
TTCCTAGTCA GCATCCCCAG TTACGAGCCC CAGGAGACAG ACAGCCTCAG CCAGATGGAG 
ACCAAGCTGA AGAACCAGAA GAACCTGCTA GATGAGATAG CAAGTAGGGA GCAGGAAGTA 
CAGAAGATCT GTGCCAATTC CCAGCAGTAC CAGCAAGCTG TAAAGGACTA TGAGTTAGAA 
GCAGAAAAAC TAAGGTCTCT TCTCGACTTG GAGAATGGAA GGAGAAGCCA CGTGAGCAAG 
AGAGCCAGGC TCCAATCTCC TGCCACCAAA GTGAAGGAAG AGGAAGCAGC ACTTGCCGCC 
AAGTTCACTG AAGTTTATGC CATCAACAGA CAGAGGCTGC AGAATCTGGA GTTTGCTCTG 
AATCTCCTCA GACAGCAGCC GGAAGTAGAA GTGACCCATG AGACCCTGCA AAGGAATAGG 
CCGGACTCTG GAGTGGAGGA GGCGTGGAAG ATCAGGAAGG AACTGGATGA GGAGACTGAG 
CGGAGGCGGC AGCTGGAGAA CGAGGTCAAG AGCACCCAGG AAGAAATCTG GACCTTGAGG 
AATCAGGGGC CTCAGGAATC GGTGGTGAGG AAGGAGGTGC TCAAGAAGGT GCCGGATCCC 
GTGCTGGAGG AGAGCTTCCA GCAGCTGCAG CGGACGCTGG CAGAGGAGCA GCACAAGAAC 
CAGCTGCTGC AGGAGGAGCT GGAGGCACTG CAGCTGCAGC TGCGTGCCCT GGAGCAGGAG 
ACCAGAGACG GGGGGCAGGA GTACGTGGTC AAGGAGGTCC TGCGCATCGA GCCTGACAGG 
GCCCAGGCGG ATGAGGTCTT GCAGCTGCGG GAGGAGCTGG AGGCACTGAG GCGGCAGAAG 
GGCGCCCGGG AGGCAGAGGT GCTCCTCCTG CAGCAGCGTG TGGCCGCCCT GGCTGAAGAG 
AAGAGCCGGG CGCAGGAGAA GGTCACAGAG AAAGAGGTGG TGAAACTGCA GAATGACCCC 
CAGCTGGAGG CAGAGTACCA GCAGCTGCAG GAGGACCACC AGCGCCAGGA CCAGCTCAGG 
GAGAAGCAGG AGGAGGAGCT GAGCTTCCTC CAGGACAAGC TCAAGAGGCT AGAGAAGGAG 
CGGGCCATGG CCGAGGGCAA GATCACCGTC AAGGAGGTGC TCAAGGTGGA GAAGGACGCG 
GCCACCGAGA GGGAGGTCAG CGATCTCACC CGCCAATATG AGGACGAGGC TGCCAAGGCT 
CGCGCTAGCC AGAGGGAGAA GACGGAGCTG CTCCGAAAGA TATGGGCCTT GGAGGAGGAG 
AACGCCAAAG TGGTGGTGCA GGAGAAGGTG CGGGAGATCG TGCGGCCAGA CCCCAAGGCG 
GAAAGTGAAG TGGCGAACCT CCGCCTGGAG CTTGTGGAGC AGGAGCGAAA GTACCGGGGT 
GCCGAGGAGC AGCTCCGGAG CTACCAGAGT GAGCTGGAGG CCCTCAGGAG GCGAGGCCCC 
CAGGTGGAAG TCAAAGAGGT GACTAAGGAA GTCATTAAGT ACAAGACTGA CCCTGAGATG 
GAGAAGGAGC TTCAGCGGCT CAGGGAGGAG ATCGTGGACA AGACCAGACT GATCGAAAGG 
TGTGATTTAG AGATCTACCA GCTGAAAAAG GAAATCCAGG CCCTGAAAGA CACCAAACCC 
CAGGTCCAGA CCAAAGAGGT GGTCCAGGAG ATCCTCCAAT TCCAAGAAGA CCCTCAAACC 
AAGGAGGAGG TGGCGTCTCT GAGGGCAAAG CTCTCAGAGG AGCAGAAGAA ACAAGTGGAT 
CTGGAGAGGG AAAGAGCTTC CCAGGAAGAG CAGATCGCCC GGAAAGAGGA GGAGCTCTCG 
CGGGTGAAGG AAAGGGTGGT GCAGCAGGAG GTGGTCAGGT ATGAGGAGGA GCCAGGCCTG 
CGGGCCGAGG CGAGCGCCTT TGCCGAGAGC ATCGATGTGG AGCTGCGGCA GATTGACAAG 
CTGCGGGCAG AGCTGCGGCG GCTGCAGCGC CGGCGCACCG AGCTTGAGCG GCAGCTGGAG 
GAGCTAGAGC GCGAGCGGCA GGCCCGCAGG GAGGCCGAGC GCGAGGTACA GCGGTTGCAG 
CAGCGGCTGG CAGCGCTGGA GCAGGAAGAA GCTGAGGCCC GTGAGAAGGT AACCCATACG 
CAGAAGGTGG TGCTGCAGCA GGACCCGCAG CAGGCGCGAG AGCATGCCCT GCTCCGACTC 
CAGCTGGAAG AAGAGCAGCA CCGGCGGCAG CTCCTGGAGG GGGAGCTCGA GACCCTCCGG 
AGGAAACTGG CTGCACTGGA GAAGGCGGAG GTCAAGGAGA AGGTGGTGCT CTCCGAGAGT 
GTCCAGGTGG AGAAGGGCGA CACCGAGCAA GAGATCCAGA GGCTCAAGAG CAGCCTGGAG 
GAGGAGAGCC GCAGCAAGCG CGAGCTGGAC GTCGAGGTGA GCCGGCTGGA AGCCAGGCTT 
TCGGAGCTGG AATTCCATAA CTCCAAGTCA TCCAAGGAAC TAGACTTTCT GAGGGAAGAG 
AACCACAAAT TACAGCTGGA GAGGCAAAAC CTGCAGCTGG AGACCCGAAG GCTCCAATCG 
GAAATCAACA TGGCAGCGAC GGAAACACGA GACCTGCGGA ACATGACCGT GGCGGACTCT 
GGGACCAACC ATGACTCCAG ACTGTGGTCC CTGGAGAGGG AACTGGATGA CCTCAAGAGG 
CTCTCCAAGG ACAAAGACCT CGAGATCGAC GAGCTGCAGA AGCGCCTGGG CTCCGTGGCC 
GTCAAGCGGG AGCAGCGGGA GAACCACCTG CGGCGCTCCA TCGTAGTCAT CCACCCTGAC 
ACAGGCCGCG AGCTGTCCCC GGAGGAAGCC CACCGTGCCG GGCTCATTGA CTGGAACATG 
TTCGTGAAAC TCAGAAGCCA GGAGTGCGAC TGGGAGGAGA TCTCAGTGAA GGGTCCCAAT 
GGGGAGTCCT CAGTGATACA CGACAGGAAG TCTGGCAAGA AGTTCTCCAT CGAAGAGGCC 
CTGCAGAGTG GCAGGCTGAC CCCTGCTCAG TATGACCGCT ATGTCAACAA GGATATGTCC 
ATCCAGGAGC TGGCGGTCTT GGTATCTGGG CAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPL ( XM_017023375.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878864.1 Homo sapiens periplakin (PPL), transcript variant X2, mRNA.

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CloneID	OHu86121
Clone ID Related Accession (Same CDS sequence)	XM_017023375.1 , XM_017023375.2
Accession Version	XM_017023375.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	periplakin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017023375.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCCACTGT GCAGACCCGG 
AGCATCTCTA ACAAGGAGCT CTCGGAGCTG ATCGAGCAGC TGCAGAAGAA TGCCGACCAG 
GTGGAGAAGA ACATCGTGGA CACAGAGGCC AAGATGCAGA GTGACCTGGC TCGGCTGCAG 
GAGGGTCGGC AGCCTGAGCA CCGGGACGTG ACCCTGCAGA AGGTGTTGGA CTCTGAGAAG 
CTGCTCTATG TGCTAGAGGC GGATGCGGCC ATTGCCAAGC ACATGAAGCA CCCACAGGGG 
GACATGATCG CCGAGGATAT CCGCCAGCTG AAGGAGCGTG TGACCAACCT GCGCGGGAAA 
CACAAGCAGA TCTACAGGCT GGCGGTGAAG GAAGTGGATC CACAGGTCAA CTGGGCGGCA 
CTGGTGGAGG AGAAGCTGGA CAAGCTGAAC AACCAGAGCT TTGGGACTGA CCTGCCGCTG 
GTGGACCACC AAGTGGAGGA GCATAACATC TTCCACAATG AGGTCAAGGC CATCGGGCCC 
CACCTGGCCA AGGACGGGGA CAAGGAGCAG AACAGCGAAC TCCGGGCCAA GTACCAGAAA 
CTGCTGGCAG CATCACAGGC CCGGCAGCAG CACCTGAGTT CGCTGCAGGA CTACATGCAG 
CGCTGCACCA ATGAGCTGTA CTGGCTGGAC CAGCAGGCCA AGGGCCGCAT GCAGTACGAC 
TGGAGTGACC GCAACCTCGA CTACCCCAGC CGCCGGCGCC AGTATGAGAA TTTCATCAAC 
CGGAACCTGG AGGCCAAAGA GGAGAGAATC AACAAACTGC ACAGCGAGGG CGACCAGCTG 
CTGGCGGCCG AGCACCCCGG GAGGAACTCC ATTGAGGCGC ACATGGAGGC TGTGCACGCA 
GACTGGAAGG AGTACCTGAA CCTGCTCATC TGCGAGGAGA GCCACCTCAA GTACATGGAG 
GACTACCACC AGTTTCACGA AGACGTGAAG GACGCTCAGG AGCTGCTGCG CAAGGTGGAC 
TCGGACCTGA ACCAGAAGTA TGGCCCTGAC TTCAAGGACC GGTACCAGAT TGAGCTGCTG 
CTGCGGGAGC TGGATGACCA GGAGAAGGTG CTGGACAAGT ATGAGGACGT GGTGCAGGGG 
CTGCAGAAGC GAGGCCAGCA GGTGGTGCCC CTCAAGTACC GCCGGGAGAC TCCGCTCAAG 
CCCATCCCCG TGGAGGCACT CTGTGACTTT GAGGGGGAGC AGGCCCTCAG GCCCTGCCGG 
GATCCTGACT GTCCAGCCTG CCCCCGCCAG GGCCTGATCT CGCGGGGCTA CAGCTACACC 
CTGCAGAAGA ACAACGGGGA GAGCTGGGAG CTCATGGACA GCGCTGGGAA CAAGCTGATT 
GCTCCGGCCG TGTGTTTTGT GATCCCCCCC ACAGACCCTG AGGCCCTGGC TCTGGCTGAC 
AGCCTGGGCA GCCAGTACCG GAGCGTGCGG CAGAAGGCAG CTGGGAGCAA ACGCACGCTG 
CAGCAGCGGT ATGAGGTGCT GAAGACCGAG AATCCCGGAG ATGCCTCTGA CCTACAGGGG 
CGGCAGCTGC TGGCTGGCTT GGACAAGGTG GCCAGCGACC TGGACCGGCA GGAGAAGGCC 
ATCACAGGGA TCCTGCGGCC ACCACTGGAG CAAGGCCGGG CTGTGCAGGA CAGTGCCGAG 
CGGGCCAAGG ACCTCAAGAA CATCACCAAC GAGCTACTGC GGATTGAACC TGAGAAGACG 
CGGAGCACGG CTGAGGGCGA AGCCTTCATC CAGGCCCTCC CAGGCAGTGG CACCACACCC 
CTGCTGAGGA CCCGGGTGGA GGACACCAAC CGGAAATACG AGCACCTCCT GCAGCTGCTG 
GACTTGGCCC AGGAGAAGGT TGATGTGGCC AACCGCCTGG AGAAGAGCCT GCAGCAGAGC 
TGGGAGTTGC TGGCCACACA CGAGAACCAT CTGAATCAGG ATGACACAGT GCCTGAGAGC 
AGCCGTGTCC TGGACAGCAA GGGGCAGGAG CTGGCGGCCA TGGCCTGTGA GTTACAGGCC 
CAGAAGTCCC TCCTGGGTGA GGTGGAGCAG AACTTGCAGG CGGCCAAGCA GTGCTCGAGC 
ACACTGGCCA GCCGCTTCCA GGAGCACTGT CCGGACCTGG AGCGCCAGGA GGCCGAGGTG 
CACAAGCTGG GCCAGCGTTT CAACAACCTG CGCCAGCAGG TGGAACGCAG GGCGCAGAGC 
CTACAGAGCG CCAAGGCAGC CTACGAGCAC TTCCACCGCG GCCATGACCA CGTGCTGCAG 
TTCCTAGTCA GCATCCCCAG TTACGAGCCC CAGGAGACAG ACAGCCTCAG CCAGATGGAG 
ACCAAGCTGA AGAACCAGAA GAACCTGCTA GATGAGATAG CAAGTAGGGA GCAGGAAGTA 
CAGAAGATCT GTGCCAATTC CCAGCAGTAC CAGCAAGCTG TAAAGGACTA TGAGTTAGAA 
GCAGAAAAAC TAAGGTCTCT TCTCGACTTG GAGAATGGAA GGAGAAGCCA CGTGAGCAAG 
AGAGCCAGGC TCCAATCTCC TGCCACCAAA GTGAAGGAAG AGGAAGCAGC ACTTGCCGCC 
AAGTTCACTG AAGTTTATGC CATCAACAGA CAGAGGCTGC AGAATCTGGA GTTTGCTCTG 
AATCTCCTCA GACAGCAGCC GGAAGTAGAA GTGACCCATG AGACCCTGCA AAGGAATAGG 
CCGGACTCTG GAGTGGAGGA GGCGTGGAAG ATCAGGAAGG AACTGGATGA GGAGACTGAG 
CGGAGGCGGC AGCTGGAGAA CGAGGTCAAG AGCACCCAGG AAGAAATCTG GACCTTGAGG 
AATCAGGGGC CTCAGGAATC GGTGGTGAGG AAGGAGGTGC TCAAGAAGGT GCCGGATCCC 
GTGCTGGAGG AGAGCTTCCA GCAGCTGCAG CGGACGCTGG CAGAGGAGCA GCACAAGAAC 
CAGCTGCTGC AGGAGGAGCT GGAGGCACTG CAGCTGCAGC TGCGTGCCCT GGAGCAGGAG 
ACCAGAGACG GGGGGCAGGA GTACGTGGTC AAGGAGGTCC TGCGCATCGA GCCTGACAGG 
GCCCAGGCGG ATGAGGTCTT GCAGCTGCGG GAGGAGCTGG AGGCACTGAG GCGGCAGAAG 
GGCGCCCGGG AGGCAGAGGT GCTCCTCCTG CAGCAGCGTG TGGCCGCCCT GGCTGAAGAG 
AAGAGCCGGG CGCAGGAGAA GGTCACAGAG AAAGAGGTGG TGAAACTGCA GAATGACCCC 
CAGCTGGAGG CAGAGTACCA GCAGCTGCAG GAGGACCACC AGCGCCAGGA CCAGCTCAGG 
GAGAAGCAGG AGGAGGAGCT GAGCTTCCTC CAGGACAAGC TCAAGAGGCT AGAGAAGGAG 
CGGGCCATGG CCGAGGGCAA GATCACCGTC AAGGAGGTGC TCAAGGTGGA GAAGGACGCG 
GCCACCGAGA GGGAGGTCAG CGATCTCACC CGCCAATATG AGGACGAGGC TGCCAAGGCT 
CGCGCTAGCC AGAGGGAGAA GACGGAGCTG CTCCGAAAGA TATGGGCCTT GGAGGAGGAG 
AACGCCAAAG TGGTGGTGCA GGAGAAGGTG CGGGAGATCG TGCGGCCAGA CCCCAAGGCG 
GAAAGTGAAG TGGCGAACCT CCGCCTGGAG CTTGTGGAGC AGGAGCGAAA GTACCGGGGT 
GCCGAGGAGC AGCTCCGGAG CTACCAGAGT GAGCTGGAGG CCCTCAGGAG GCGAGGCCCC 
CAGGTGGAAG TCAAAGAGGT GACTAAGGAA GTCATTAAGT ACAAGACTGA CCCTGAGATG 
GAGAAGGAGC TTCAGCGGCT CAGGGAGGAG ATCGTGGACA AGACCAGACT GATCGAAAGG 
TGTGATTTAG AGATCTACCA GCTGAAAAAG GAAATCCAGG CCCTGAAAGA CACCAAACCC 
CAGGTCCAGA CCAAAGAGGT GGTCCAGGAG ATCCTCCAAT TCCAAGAAGA CCCTCAAACC 
AAGGAGGAGG TGGCGTCTCT GAGGGCAAAG CTCTCAGAGG AGCAGAAGAA ACAAGTGGAT 
CTGGAGAGGG AAAGAGCTTC CCAGGAAGAG CAGATCGCCC GGAAAGAGGA GGAGCTCTCG 
CGGGTGAAGG AAAGGGTGGT GCAGCAGGAG GTGGTCAGGT ATGAGGAGGA GCCAGGCCTG 
CGGGCCGAGG CGAGCGCCTT TGCCGAGAGC ATCGATGTGG AGCTGCGGCA GATTGACAAG 
CTGCGGGCAG AGCTGCGGCG GCTGCAGCGC CGGCGCACCG AGCTTGAGCG GCAGCTGGAG 
GAGCTAGAGC GCGAGCGGCA GGCCCGCAGG GAGGCCGAGC GCGAGGTACA GCGGTTGCAG 
CAGCGGCTGG CAGCGCTGGA GCAGGAAGAA GCTGAGGCCC GTGAGAAGGT AACCCATACG 
CAGAAGGTGG TGCTGCAGCA GGACCCGCAG CAGGCGCGAG AGCATGCCCT GCTCCGACTC 
CAGCTGGAAG AAGAGCAGCA CCGGCGGCAG CTCCTGGAGG GGGAGCTCGA GACCCTCCGG 
AGGAAACTGG CTGCACTGGA GAAGGCGGAG GTCAAGGAGA AGGTGGTGCT CTCCGAGAGT 
GTCCAGGTGG AGAAGGGCGA CACCGAGCAA GAGATCCAGA GGCTCAAGAG CAGCCTGGAG 
GAGGAGAGCC GCAGCAAGCG CGAGCTGGAC GTCGAGGTGA GCCGGCTGGA AGCCAGGCTT 
TCGGAGCTGG AATTCCATAA CTCCAAGTCA TCCAAGGAAC TAGACTTTCT GAGGGAAGAG 
AACCACAAAT TACAGCTGGA GAGGCAAAAC CTGCAGCTGG AGACCCGAAG GCTCCAATCG 
GAAATCAACA TGGCAGCGAC GGAAACACGA GACCTGCGGA ACATGACCGT GGCGGACTCT 
GGGACCAACC ATGACTCCAG ACTGTGGTCC CTGGAGAGGG AACTGGATGA CCTCAAGAGG 
CTCTCCAAGG ACAAAGACCT CGAGATCGAC GAGCTGCAGA AGCGCCTGGG CTCCGTGGCC 
GTCAAGCGGG AGCAGCGGGA GAACCACCTG CGGCGCTCCA TCGTAGTCAT CCACCCTGAC 
ACAGGCCGCG AGCTGTCCCC GGAGGAAGCC CACCGTGCCG GGCTCATTGA CTGGAACATG 
TTCGTGAAAC TCAGAAGCCA GGAGTGCGAC TGGGAGGAGA TCTCAGTGAA GGGTCCCAAT 
GGGGAGTCCT CAGTGATACA CGACAGGAAG TCTGGCAAGA AGTTCTCCAT CGAAGAGGCC 
CTGCAGAGTG GCAGGCTGAC CCCTGCTCAG TATGACCGCT ATGTCAACAA GGATATGTCC 
ATCCAGGAGC TGGCGGTCTT GGTATCTGGG CAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878864.1
CDS	67..5385
Translation	MNSLFRKRNKGKYSPTVQTRSISNKELSELIEQLQKNADQVEKNIVDTEAKMQSDLARLQEGRQPEHRDVTLQKVLDSEKLLYVLEADAAIAKHMKHPQGDMIAEDIRQLKERVTNLRGKHKQIYRLAVKEVDPQVNWAALVEEKLDKLNNQSFGTDLPLVDHQVEEHNIFHNEVKAIGPHLAKDGDKEQNSELRAKYQKLLAASQARQQHLSSLQDYMQRCTNELYWLDQQAKGRMQYDWSDRNLDYPSRRRQYENFINRNLEAKEERINKLHSEGDQLLAAEHPGRNSIEAHMEAVHADWKEYLNLLICEESHLKYMEDYHQFHEDVKDAQELLRKVDSDLNQKYGPDFKDRYQIELLLRELDDQEKVLDKYEDVVQGLQKRGQQVVPLKYRRETPLKPIPVEALCDFEGEQALRPCRDPDCPACPRQGLISRGYSYTLQKNNGESWELMDSAGNKLIAPAVCFVIPPTDPEALALADSLGSQYRSVRQKAAGSKRTLQQRYEVLKTENPGDASDLQGRQLLAGLDKVASDLDRQEKAITGILRPPLEQGRAVQDSAERAKDLKNITNELLRIEPEKTRSTAEGEAFIQALPGSGTTPLLRTRVEDTNRKYEHLLQLLDLAQEKVDVANRLEKSLQQSWELLATHENHLNQDDTVPESSRVLDSKGQELAAMACELQAQKSLLGEVEQNLQAAKQCSSTLASRFQEHCPDLERQEAEVHKLGQRFNNLRQQVERRAQSLQSAKAAYEHFHRGHDHVLQFLVSIPSYEPQETDSLSQMETKLKNQKNLLDEIASREQEVQKICANSQQYQQAVKDYELEAEKLRSLLDLENGRRSHVSKRARLQSPATKVKEEEAALAAKFTEVYAINRQRLQNLEFALNLLRQQPEVEVTHETLQRNRPDSGVEEAWKIRKELDEETERRRQLENEVKSTQEEIWTLRNQGPQESVVRKEVLKKVPDPVLEESFQQLQRTLAEEQHKNQLLQEELEALQLQLRALEQETRDGGQEYVVKEVLRIEPDRAQADEVLQLREELEALRRQKGAREAEVLLLQQRVAALAEEKSRAQEKVTEKEVVKLQNDPQLEAEYQQLQEDHQRQDQLREKQEEELSFLQDKLKRLEKERAMAEGKITVKEVLKVEKDAATEREVSDLTRQYEDEAAKARASQREKTELLRKIWALEEENAKVVVQEKVREIVRPDPKAESEVANLRLELVEQERKYRGAEEQLRSYQSELEALRRRGPQVEVKEVTKEVIKYKTDPEMEKELQRLREEIVDKTRLIERCDLEIYQLKKEIQALKDTKPQVQTKEVVQEILQFQEDPQTKEEVASLRAKLSEEQKKQVDLERERASQEEQIARKEEELSRVKERVVQQEVVRYEEEPGLRAEASAFAESIDVELRQIDKLRAELRRLQRRRTELERQLEELERERQARREAEREVQRLQQRLAALEQEEAEAREKVTHTQKVVLQQDPQQAREHALLRLQLEEEQHRRQLLEGELETLRRKLAALEKAEVKEKVVLSESVQVEKGDTEQEIQRLKSSLEEESRSKRELDVEVSRLEARLSELEFHNSKSSKELDFLREENHKLQLERQNLQLETRRLQSEINMAATETRDLRNMTVADSGTNHDSRLWSLERELDDLKRLSKDKDLEIDELQKRLGSVAVKREQRENHLRRSIVVIHPDTGRELSPEEAHRAGLIDWNMFVKLRSQECDWEEISVKGPNGESSVIHDRKSGKKFSIEEALQSGRLTPAQYDRYVNKDMSIQELAVLVSGQK

Target ORF information:

RefSeq Version	XM_017023375.2
Organism	Homo sapiens(human)
Definition	Homo sapiens periplakin (PPL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023375.2

ORF Insert Sequence:

ATGAACTCGC TCTTCAGGAA GAGAAACAAA GGCAAATACA GCCCCACTGT GCAGACCCGG 
AGCATCTCTA ACAAGGAGCT CTCGGAGCTG ATCGAGCAGC TGCAGAAGAA TGCCGACCAG 
GTGGAGAAGA ACATCGTGGA CACAGAGGCC AAGATGCAGA GTGACCTGGC TCGGCTGCAG 
GAGGGTCGGC AGCCTGAGCA CCGGGACGTG ACCCTGCAGA AGGTGTTGGA CTCTGAGAAG 
CTGCTCTATG TGCTAGAGGC GGATGCGGCC ATTGCCAAGC ACATGAAGCA CCCACAGGGG 
GACATGATCG CCGAGGATAT CCGCCAGCTG AAGGAGCGTG TGACCAACCT GCGCGGGAAA 
CACAAGCAGA TCTACAGGCT GGCGGTGAAG GAAGTGGATC CACAGGTCAA CTGGGCGGCA 
CTGGTGGAGG AGAAGCTGGA CAAGCTGAAC AACCAGAGCT TTGGGACTGA CCTGCCGCTG 
GTGGACCACC AAGTGGAGGA GCATAACATC TTCCACAATG AGGTCAAGGC CATCGGGCCC 
CACCTGGCCA AGGACGGGGA CAAGGAGCAG AACAGCGAAC TCCGGGCCAA GTACCAGAAA 
CTGCTGGCAG CATCACAGGC CCGGCAGCAG CACCTGAGTT CGCTGCAGGA CTACATGCAG 
CGCTGCACCA ATGAGCTGTA CTGGCTGGAC CAGCAGGCCA AGGGCCGCAT GCAGTACGAC 
TGGAGTGACC GCAACCTCGA CTACCCCAGC CGCCGGCGCC AGTATGAGAA TTTCATCAAC 
CGGAACCTGG AGGCCAAAGA GGAGAGAATC AACAAACTGC ACAGCGAGGG CGACCAGCTG 
CTGGCGGCCG AGCACCCCGG GAGGAACTCC ATTGAGGCGC ACATGGAGGC TGTGCACGCA 
GACTGGAAGG AGTACCTGAA CCTGCTCATC TGCGAGGAGA GCCACCTCAA GTACATGGAG 
GACTACCACC AGTTTCACGA AGACGTGAAG GACGCTCAGG AGCTGCTGCG CAAGGTGGAC 
TCGGACCTGA ACCAGAAGTA TGGCCCTGAC TTCAAGGACC GGTACCAGAT TGAGCTGCTG 
CTGCGGGAGC TGGATGACCA GGAGAAGGTG CTGGACAAGT ATGAGGACGT GGTGCAGGGG 
CTGCAGAAGC GAGGCCAGCA GGTGGTGCCC CTCAAGTACC GCCGGGAGAC TCCGCTCAAG 
CCCATCCCCG TGGAGGCACT CTGTGACTTT GAGGGGGAGC AGGCCCTCAG GCCCTGCCGG 
GATCCTGACT GTCCAGCCTG CCCCCGCCAG GGCCTGATCT CGCGGGGCTA CAGCTACACC 
CTGCAGAAGA ACAACGGGGA GAGCTGGGAG CTCATGGACA GCGCTGGGAA CAAGCTGATT 
GCTCCGGCCG TGTGTTTTGT GATCCCCCCC ACAGACCCTG AGGCCCTGGC TCTGGCTGAC 
AGCCTGGGCA GCCAGTACCG GAGCGTGCGG CAGAAGGCAG CTGGGAGCAA ACGCACGCTG 
CAGCAGCGGT ATGAGGTGCT GAAGACCGAG AATCCCGGAG ATGCCTCTGA CCTACAGGGG 
CGGCAGCTGC TGGCTGGCTT GGACAAGGTG GCCAGCGACC TGGACCGGCA GGAGAAGGCC 
ATCACAGGGA TCCTGCGGCC ACCACTGGAG CAAGGCCGGG CTGTGCAGGA CAGTGCCGAG 
CGGGCCAAGG ACCTCAAGAA CATCACCAAC GAGCTACTGC GGATTGAACC TGAGAAGACG 
CGGAGCACGG CTGAGGGCGA AGCCTTCATC CAGGCCCTCC CAGGCAGTGG CACCACACCC 
CTGCTGAGGA CCCGGGTGGA GGACACCAAC CGGAAATACG AGCACCTCCT GCAGCTGCTG 
GACTTGGCCC AGGAGAAGGT TGATGTGGCC AACCGCCTGG AGAAGAGCCT GCAGCAGAGC 
TGGGAGTTGC TGGCCACACA CGAGAACCAT CTGAATCAGG ATGACACAGT GCCTGAGAGC 
AGCCGTGTCC TGGACAGCAA GGGGCAGGAG CTGGCGGCCA TGGCCTGTGA GTTACAGGCC 
CAGAAGTCCC TCCTGGGTGA GGTGGAGCAG AACTTGCAGG CGGCCAAGCA GTGCTCGAGC 
ACACTGGCCA GCCGCTTCCA GGAGCACTGT CCGGACCTGG AGCGCCAGGA GGCCGAGGTG 
CACAAGCTGG GCCAGCGTTT CAACAACCTG CGCCAGCAGG TGGAACGCAG GGCGCAGAGC 
CTACAGAGCG CCAAGGCAGC CTACGAGCAC TTCCACCGCG GCCATGACCA CGTGCTGCAG 
TTCCTAGTCA GCATCCCCAG TTACGAGCCC CAGGAGACAG ACAGCCTCAG CCAGATGGAG 
ACCAAGCTGA AGAACCAGAA GAACCTGCTA GATGAGATAG CAAGTAGGGA GCAGGAAGTA 
CAGAAGATCT GTGCCAATTC CCAGCAGTAC CAGCAAGCTG TAAAGGACTA TGAGTTAGAA 
GCAGAAAAAC TAAGGTCTCT TCTCGACTTG GAGAATGGAA GGAGAAGCCA CGTGAGCAAG 
AGAGCCAGGC TCCAATCTCC TGCCACCAAA GTGAAGGAAG AGGAAGCAGC ACTTGCCGCC 
AAGTTCACTG AAGTTTATGC CATCAACAGA CAGAGGCTGC AGAATCTGGA GTTTGCTCTG 
AATCTCCTCA GACAGCAGCC GGAAGTAGAA GTGACCCATG AGACCCTGCA AAGGAATAGG 
CCGGACTCTG GAGTGGAGGA GGCGTGGAAG ATCAGGAAGG AACTGGATGA GGAGACTGAG 
CGGAGGCGGC AGCTGGAGAA CGAGGTCAAG AGCACCCAGG AAGAAATCTG GACCTTGAGG 
AATCAGGGGC CTCAGGAATC GGTGGTGAGG AAGGAGGTGC TCAAGAAGGT GCCGGATCCC 
GTGCTGGAGG AGAGCTTCCA GCAGCTGCAG CGGACGCTGG CAGAGGAGCA GCACAAGAAC 
CAGCTGCTGC AGGAGGAGCT GGAGGCACTG CAGCTGCAGC TGCGTGCCCT GGAGCAGGAG 
ACCAGAGACG GGGGGCAGGA GTACGTGGTC AAGGAGGTCC TGCGCATCGA GCCTGACAGG 
GCCCAGGCGG ATGAGGTCTT GCAGCTGCGG GAGGAGCTGG AGGCACTGAG GCGGCAGAAG 
GGCGCCCGGG AGGCAGAGGT GCTCCTCCTG CAGCAGCGTG TGGCCGCCCT GGCTGAAGAG 
AAGAGCCGGG CGCAGGAGAA GGTCACAGAG AAAGAGGTGG TGAAACTGCA GAATGACCCC 
CAGCTGGAGG CAGAGTACCA GCAGCTGCAG GAGGACCACC AGCGCCAGGA CCAGCTCAGG 
GAGAAGCAGG AGGAGGAGCT GAGCTTCCTC CAGGACAAGC TCAAGAGGCT AGAGAAGGAG 
CGGGCCATGG CCGAGGGCAA GATCACCGTC AAGGAGGTGC TCAAGGTGGA GAAGGACGCG 
GCCACCGAGA GGGAGGTCAG CGATCTCACC CGCCAATATG AGGACGAGGC TGCCAAGGCT 
CGCGCTAGCC AGAGGGAGAA GACGGAGCTG CTCCGAAAGA TATGGGCCTT GGAGGAGGAG 
AACGCCAAAG TGGTGGTGCA GGAGAAGGTG CGGGAGATCG TGCGGCCAGA CCCCAAGGCG 
GAAAGTGAAG TGGCGAACCT CCGCCTGGAG CTTGTGGAGC AGGAGCGAAA GTACCGGGGT 
GCCGAGGAGC AGCTCCGGAG CTACCAGAGT GAGCTGGAGG CCCTCAGGAG GCGAGGCCCC 
CAGGTGGAAG TCAAAGAGGT GACTAAGGAA GTCATTAAGT ACAAGACTGA CCCTGAGATG 
GAGAAGGAGC TTCAGCGGCT CAGGGAGGAG ATCGTGGACA AGACCAGACT GATCGAAAGG 
TGTGATTTAG AGATCTACCA GCTGAAAAAG GAAATCCAGG CCCTGAAAGA CACCAAACCC 
CAGGTCCAGA CCAAAGAGGT GGTCCAGGAG ATCCTCCAAT TCCAAGAAGA CCCTCAAACC 
AAGGAGGAGG TGGCGTCTCT GAGGGCAAAG CTCTCAGAGG AGCAGAAGAA ACAAGTGGAT 
CTGGAGAGGG AAAGAGCTTC CCAGGAAGAG CAGATCGCCC GGAAAGAGGA GGAGCTCTCG 
CGGGTGAAGG AAAGGGTGGT GCAGCAGGAG GTGGTCAGGT ATGAGGAGGA GCCAGGCCTG 
CGGGCCGAGG CGAGCGCCTT TGCCGAGAGC ATCGATGTGG AGCTGCGGCA GATTGACAAG 
CTGCGGGCAG AGCTGCGGCG GCTGCAGCGC CGGCGCACCG AGCTTGAGCG GCAGCTGGAG 
GAGCTAGAGC GCGAGCGGCA GGCCCGCAGG GAGGCCGAGC GCGAGGTACA GCGGTTGCAG 
CAGCGGCTGG CAGCGCTGGA GCAGGAAGAA GCTGAGGCCC GTGAGAAGGT AACCCATACG 
CAGAAGGTGG TGCTGCAGCA GGACCCGCAG CAGGCGCGAG AGCATGCCCT GCTCCGACTC 
CAGCTGGAAG AAGAGCAGCA CCGGCGGCAG CTCCTGGAGG GGGAGCTCGA GACCCTCCGG 
AGGAAACTGG CTGCACTGGA GAAGGCGGAG GTCAAGGAGA AGGTGGTGCT CTCCGAGAGT 
GTCCAGGTGG AGAAGGGCGA CACCGAGCAA GAGATCCAGA GGCTCAAGAG CAGCCTGGAG 
GAGGAGAGCC GCAGCAAGCG CGAGCTGGAC GTCGAGGTGA GCCGGCTGGA AGCCAGGCTT 
TCGGAGCTGG AATTCCATAA CTCCAAGTCA TCCAAGGAAC TAGACTTTCT GAGGGAAGAG 
AACCACAAAT TACAGCTGGA GAGGCAAAAC CTGCAGCTGG AGACCCGAAG GCTCCAATCG 
GAAATCAACA TGGCAGCGAC GGAAACACGA GACCTGCGGA ACATGACCGT GGCGGACTCT 
GGGACCAACC ATGACTCCAG ACTGTGGTCC CTGGAGAGGG AACTGGATGA CCTCAAGAGG 
CTCTCCAAGG ACAAAGACCT CGAGATCGAC GAGCTGCAGA AGCGCCTGGG CTCCGTGGCC 
GTCAAGCGGG AGCAGCGGGA GAACCACCTG CGGCGCTCCA TCGTAGTCAT CCACCCTGAC 
ACAGGCCGCG AGCTGTCCCC GGAGGAAGCC CACCGTGCCG GGCTCATTGA CTGGAACATG 
TTCGTGAAAC TCAGAAGCCA GGAGTGCGAC TGGGAGGAGA TCTCAGTGAA GGGTCCCAAT 
GGGGAGTCCT CAGTGATACA CGACAGGAAG TCTGGCAAGA AGTTCTCCAT CGAAGAGGCC 
CTGCAGAGTG GCAGGCTGAC CCCTGCTCAG TATGACCGCT ATGTCAACAA GGATATGTCC 
ATCCAGGAGC TGGCGGTCTT GGTATCTGGG CAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of periplakin expression is associated with the tumorigenesis of colorectal carcinoma.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie87366-374(2017 Mar)
Li X,Zhang G,Wang Y,Elgehama A,Sun Y,Li L,Gu Y,Guo W,Xu Q

Perip7lakin is a target for autoimmunity in asthma.
Respiratory research17(1)126(2016 Oct)
Taill? C,Grootenboer-Mignot S,Estellat C,Roy C,Ly Ka So S,Pretolani M,Aubier M,Crestani B,Chollet-Martin S

Activation of human ?? T cells by cytosolic interactions of BTN3A1 with soluble phosphoantigens and the cytoskeletal adaptor periplakin.
Journal of immunology (Baltimore, Md. : 1950)194(5)2390-8(2015 Mar)
Rhodes DA,Chen HC,Price AJ,Keeble AH,Davey MS,James LC,Eberl M,Trowsdale J

Serum periplakin as a potential biomarker for urothelial carcinoma of the urinary bladder.
Asian Pacific journal of cancer prevention : APJCP15(22)9927-31(2014)
Matsumoto K,Ikeda M,Matsumoto T,Nagashio R,Nishimori T,Tomonaga T,Nomura F,Sato Y,Kitasato H,Iwamura M

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PPL cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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