Hrk cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hrk
Entrez Gene ID	12123
Full Name	harakiri, BCL2 interacting protein (contains only BH3 domain)
Synonyms	AI838259,Bid3,DP5,harakiri
General protein information	Preferred Names harakiri, BCL2 interacting protein (contains only BH3 domain) Names activator of apoptosis harakiri BH3 interacting (with BCL2 family) domain, apoptosis agonist BH3-interacting domain-containing protein 3 neuronal death protein DP5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 F

mRNA and Protein(s)

mRNA	Protein	Name
NM_007545.2	NP_031571.1	activator of apoptosis harakiri

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Hrk	NP_476471.1
Mus musculus (house mouse)	Hrk	NP_031571.1

Gene Ontology
Bibliography

Related articles in PubMed

GATA3 controls the specification of prosensory domain and neuronal survival in the mouse cochlea.
Luo XJ, Deng M, Xie X, Huang L, Wang H, Jiang L, Liang G, Hu F, Tieu R, Chen R, Gan L
Human molecular genetics22(18)3609-23(2013 Sep)

JUN regulates early transcriptional responses to axonal injury in retinal ganglion cells.
Fernandes KA, Harder JM, Kim J, Libby RT
Experimental eye research112106-17(2013 Jul)

Death protein 5 and p53-upregulated modulator of apoptosis mediate the endoplasmic reticulum stress-mitochondrial dialog triggering lipotoxic rodent and human ?-cell apoptosis.
Cunha DA, Igoillo-Esteve M, Gurzov EN, Germano CM, Naamane N, Marhfour I, Fukaya M, Vanderwinden JM, Gysemans C, Mathieu C, Marselli L, Marchetti P, Harding HP, Ron D, Eizirik DL, Cnop M
Diabetes61(11)2763-75(2012 Nov)

The Bcl-2 family member BIM has multiple glaucoma-relevant functions in DBA/2J mice.
Harder JM, Fernandes KA, Libby RT
Scientific reports2530(2012)

[The effect of antisense oligonucleoties specific to the harakiri mRNA on spontaneous and induced defects of mouse preimplantation embryo development].
Semenova ML, Zakharova EE, Zaletov SIu, Zaeva VV, Kosheleva NV
Genetika44(9)1290-3(2008 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hrk deficiency does not significantly attenuate the widespread apoptosis seen in the Bcl-x (-/-) embryonic nervous system, indicating that other BH3-only molecules, alone or in combination, may regulate BAX activation in immature neurons.
Title: Deficiency of pro-apoptotic Hrk attenuates programmed cell death in the developing murine nervous system but does not affect Bcl-x deficiency-induced neuron apoptosis.

The effect of antisense oligonucleoties specific to the harakiri mRNA on spontaneous and induced defects of mouse preimplantation embryo development
Title: [The effect of antisense oligonucleoties specific to the harakiri mRNA on spontaneous and induced defects of mouse preimplantation embryo development].

in response to PAHs, Ahr-mediated activation of the harakiri, BCL2 interacting protein (contains only BH3 domain), was necessary for execution of cell death.
Title: Maternal exposure to polycyclic aromatic hydrocarbons diminishes murine ovarian reserve via induction of Harakiri.

These results demonstrate that HRK contributes to apoptosis signalling elicited by trophic factor withdrawal in certain neuronal populations but is dispensable for apoptosis of haematopoietic cells.
Title: Hrk/DP5 contributes to the apoptosis of select neuronal populations but is dispensable for haematopoietic cell apoptosis.

DP5 plays an important role in neuronal cell death induced by axotomy and NGF deprivation through the regulation of mitochondrial function and caspase-3 activation
Title: Critical role for DP5/Harakiri, a Bcl-2 homology domain 3-only Bcl-2 family member, in axotomy-induced neuronal cell death.

The following Hrk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hrk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18036	NM_007545.2 Latest version!	Mus musculus harakiri, BCL2 interacting protein (contains only BH3 domain) (Hrk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hrk ( NM_007545.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031571.1 Mus musculus harakiri, BCL2 interacting protein (contains only BH3 domain) (Hrk), mRNA.

Price & Availability↑

CloneID	OMu18036
Clone ID Related Accession (Same CDS sequence)	NM_007545.2
Accession Version	NM_007545.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	activator of apoptosis harakiri
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from D83698.1 and AC110254.11. On Feb 16, 2006 this sequence version replaced NM_007545.1. CCDS Note: This CCDS ID represents the protein described in PMID: 9555074 and 15084651. This transcript is supported by D83698.1. It should be noted this transcript is predicted to undergo nonsense-mediated mRNA decay (NMD). However, the protein is represented because it was detected endogenously PMID: 9555074. It is likely that the majority of transcripts representing this variant will undergo NMD, while some low level of NMD escape may allow for the expression of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D83698.1, SRR1660813.84025.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## NMD candidate :: translation inferred from conservation ##RefSeq-Attributes-END##

ATGTGCCCGT GTCCCCGGCA TCGCGGCCGC GGGCCCCCGG CCGTGTGCGG TTGCGGCGAC 
GCTCGCCCCG GGCTGCGCTG GGCGGCGGCG CAGGTGACCG CGCTGCGGCT GCAGGCGCTG 
GGCGACGAGC TGCACCGACG CGCCATGCGG CGTCGCGCGC GGCCCCGGGA CCCGCTGCCC 
GCGCTGCTGC CCGCGCTCCG CGCCCGCTGG CCCTGGCTGT GCGCGGCCGC GCAGGTGGCG 
GCGCTGGCGG CCTGGCTGCT CGGCAGGCGG AGCGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031571.1
CDS	20..298
Translation	MCPCPRHRGRGPPAVCGCGDARPGLRWAAAQVTALRLQALGDELHRRAMRRRARPRDPLPALLPALRARWPWLCAAAQVAALAAWLLGRRSA

Target ORF information:

RefSeq Version	NM_007545.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus harakiri, BCL2 interacting protein (contains only BH3 domain) (Hrk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007545.2

ORF Insert Sequence:

ATGTGCCCGT GTCCCCGGCA TCGCGGCCGC GGGCCCCCGG CCGTGTGCGG TTGCGGCGAC 
GCTCGCCCCG GGCTGCGCTG GGCGGCGGCG CAGGTGACCG CGCTGCGGCT GCAGGCGCTG 
GGCGACGAGC TGCACCGACG CGCCATGCGG CGTCGCGCGC GGCCCCGGGA CCCGCTGCCC 
GCGCTGCTGC CCGCGCTCCG CGCCCGCTGG CCCTGGCTGT GCGCGGCCGC GCAGGTGGCG 
GCGCTGGCGG CCTGGCTGCT CGGCAGGCGG AGCGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

GATA3 controls the specification of prosensory domain and neuronal survival in the mouse cochlea.
Human molecular genetics22(18)3609-23(2013 Sep)
Luo XJ,Deng M,Xie X,Huang L,Wang H,Jiang L,Liang G,Hu F,Tieu R,Chen R,Gan L

JUN regulates early transcriptional responses to axonal injury in retinal ganglion cells.
Experimental eye research112106-17(2013 Jul)
Fernandes KA,Harder JM,Kim J,Libby RT

Death protein 5 and p53-upregulated modulator of apoptosis mediate the endoplasmic reticulum stress-mitochondrial dialog triggering lipotoxic rodent and human ?-cell apoptosis.
Diabetes61(11)2763-75(2012 Nov)
Cunha DA,Igoillo-Esteve M,Gurzov EN,Germano CM,Naamane N,Marhfour I,Fukaya M,Vanderwinden JM,Gysemans C,Mathieu C,Marselli L,Marchetti P,Harding HP,Ron D,Eizirik DL,Cnop M

The Bcl-2 family member BIM has multiple glaucoma-relevant functions in DBA/2J mice.
Scientific reports2530(2012)
Harder JM,Fernandes KA,Libby RT

[The effect of antisense oligonucleoties specific to the harakiri mRNA on spontaneous and induced defects of mouse preimplantation embryo development].
Genetika44(9)1290-3(2008 Sep)
Semenova ML,Zakharova EE,Zaletov SIu,Zaeva VV,Kosheleva NV

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Hrk cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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