Arhgef28 cDNA ORF clone, Mus musculus(house mouse)

The following Arhgef28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Arhgef28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu48947 XM_006517519.4
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu48949 XM_006517521.4
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu78410 XM_017315362.2
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu86213 XM_030247128.1
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu48950 XM_006517522.3
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu48946 XM_006517518.3
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu78409 XM_017315361.2
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu09358 NM_012026.2
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
View Old Accession Versions >>
OMu48949 XM_006517521.3 Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu48947 XM_006517519.3 Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu48946 XM_006517518.2 Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu78409 XM_017315361.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu78410 XM_017315362.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu48947
    Clone ID Related Accession (Same CDS sequence) XM_006517519.4 , XM_006517519.3
    Accession Version XM_006517519.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4164bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 28 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006517519.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGATTCTG ACTCGGACTC ACCTTTCAAC TACTCCTGGC CTTCCTTCCC CAAGATGAAG 
    ATTCGGCGCA GGGCATCCAA GAAAGATCGC TCCTTTGATG GCCTCAAAAA ATCCAAGCAT
    GTCCCAGCGT CCCTTGCTGC AGGCCAGCTC TCTGATGTTC TGAACGGGGG TGATGAAGTC
    TACGCTAACT GTATGGTGAT TGACCAGGTT GGCGATTTGG ATATCAACTA TATAAACCTA
    GAGGGACTCT CCACCCATAC GAGTCCCGAG TCTGGGAGGT CCATGCTGGG GCCTCAGGCT
    TGCATGCACA CCCTTCCTCC CGACACCAGT CCCTGCGGGC GCCCACTCAT TGAGAACAGT
    GAGGGGACCC TGGATGCTGC AGCTTCGCAG TCTTTCGTGA CACCATCCAG TTCTCGCACT
    TCCAACTTGA ACCTTTCTTT TGGTCTCCAT GGATTTGAAA AGGAGCAGAG TCATCTCAAG
    AAAAGGAGTT CTAGCCTGGA TGCCCTGGTT GCCGACAGTG AGGGGGAAGG AGGTTCTGAG
    CCCCCCATCT GCTATGCTGT GGGCTCTCAG AGTTCCCCAA GAACTGGACT CCCCAGCGGG
    GATGAACTGG ACTCTTTTGA GACCAACACT GAACCAGACT GTAATATCTC CAGGACCGAA
    TCACTCTCTC TGTCCAGTAC TCTGCATTCA AAGGAGTCCT TGCTTTCTGG GATCCGGTCT
    CGCTCCTACT CCTGCTCATC ACCCAAAATC TCTTCAGGAA AATCCCGGCT AGTGCGCGAC
    TTCACAGTGT GCAGTACCAG CGAAGAACAG AGATCATATA GTTTCCAGGA GCCACCAGGA
    GAAAAAAGGA TTCAGGAAGA AGAATGGGAT GAATACGTCA TTCCTGCCAA ATCCGAGTCT
    GAAAAGTACA AAGTGAGCCG AACTTTCAGC TTCCTCATGA ACAGGATGAC CAGCCCACGG
    AACAAATCTA AGATGAAAAA CAAGGACACT AAAGAAAAAG AAAAAATGAA TCGACATCAG
    TTTGTCCCGG GAACGTTCTC CGGGGTTCTG CAGTGCTCCG GTTGTGATAA AACACTCCTG
    GGGAAAGAGT CACTCCAGTG TGCCAACTGT AAGGCAAACA CACACAAGGG CTGCAAAGAT
    GCTGTGCCTC CGTGTACCAA GAAATTCCAA GAGAAATACA ACAAGAACAA ACCACAGAGC
    ATCCTTGGAA GCTCTTCAGT CAGAGACGTC CCAGCACCTG GGCTCTCCTT GCACCCTTCT
    TCCTCTATGC CCATCGGACT GCCAGCTGGA AGGAAGGAAT TTGCGGCTCA GGTGCATCCA
    CTGTCCAGAA GTGTCCCCGG CACCACTTTG GAAAGCTTCA GGAGGGCAGT GACATCGTTA
    GAGTCTGAGG GTGACAGCTG GAGAAGCAGG TCTCATTCCG ATGAGCTGTT CCAGTCTATG
    GGCTCTTCTC CTTCCACGGA GTCATTCATG ATGGAAGATG TTGTGGATTC TTCTCTGTGG
    ATCGACCTCA GTAGTGATGC TCAGGAGTTT GAAGCAGAGT CTTGGAGTCT CGTGGTGGAC
    CCTTCCTTTT GCAGTAGGCA GGAGAAGGAT GTCATCAAAA GACAAGATGT CATTTTTGAG
    CTGATGCAGA CAGAGGTCCA CCACATCCAG ACTCTGCTCA TCATGTCCGA GGTCTTCAGG
    AAAGGCATGA AGGAGGAACT GCAGCTGGAC CACAGCACGG TGGACAAGAT CTTCCCCTGC
    TTAGATGAGC TGCTGGAGAC TCACCGGCAC TTCTTCTTCA GCATGAAGGA GCGCAGGCAG
    GAGTCCTGCG CTGGCAGTGA CAGGAACTTT GTGATCAACC AAATTGGAGA CATTCTGGTA
    CAACAGTTTT CAGAGGAAAA TGCAAGCAAA ATGAAGAGGA TCTATGGAGA ATTCTGCAGC
    CATCACAAAG AAGCCATGAG TCTCTTTAAA GAGCTGCAGC AGAACAAGAA GTTTCAGAAT
    TTTATTAAGA TCCGAAATAG CAACCTGTTG GCTCGTCGCC GCGGAATCCC GGAGTGCATC
    CTGCTAGTTA CCCAGCGCAT CACAAAGTAC CCAGTCTTGG TGGAAAGGAT CCTGCAGTAC
    ACCAAGGAAA GGACTGAAGA GCACCGGGAC CTGTGCAAAG CTCTCGGCTT AATTAAAGAC
    ATGATCGCAG CCGTGGACCT GAAAGTCAGC GAATACGAGA AGAACCAAAA ATGGCTCGAG
    ATCCTAAATA AAATCGAGAA CAAAACATAC ACAAAGCTCA AAAATGGCCA CGTGTTTAGA
    AAGCAGGCGC TGCTGAGCCA GGAACGGGCT CTCCTGCATG ATGGGCTTGT CTACTGGAAA
    ACCGCTACGG GTCGCTTCAA AGACATCCTG GCTCTGCTAC TAACTGACGT GCTGCTCTTC
    TTACAAGAAA AAGACCAGAA ATACATCTTT GCAGCTGTTG ACCAGAAGCC ATCAGTGATT
    TCCCTCCAGA AGCTGATTGC TAGAGAAGTT GCCAACGAGG AGAGAGGCAT GTTTCTGATC
    AGTGCTTCGT CAGCCGGCCC CGAGATGTAC GAAATCCACA CCAATTCCAA GGAGGAGCGG
    AATAACTGGA TGAGGCGGAT CCAGCAGGCA GTAGAAAGTT GTCCAGAGGA AGAAGGAGGA
    AGGACGAGCG AGTCGGATGA AGAGAGGCGG AAAGCTGAAG CTAGAGTGGC CAAGATTCAG
    CAATGTCAAG AAATACTCAG TAACCAAGAC CAACAGATTT GCACGTACTT GGAAGAAAAG
    CTGCATATCT ACGCTGAACT TGGGGAACTG AGTGGCTTTG AGGATGTCCA CCTAGAACCC
    CATCTGCTCA TCAAACCTGA CCCTGGAGAG CCTCCCCAGG CAGCGTCCCT GCTTGCAGCA
    GCCCTGAGAG AAGCCGAGAG TCTGCAAGTG GCTGTGAAGG CTTCAAAGAT GGGCGATGTA
    AGCCAGTCAT CCGAAGAAAG CCCTGGAGGG ACTGTCCTGA TGGACACACC CAGTACACAG
    GATGTACCAG CGTCACCAAC TGCTTCATTA GTCACAGAAG GGACAGAAGG AAGAGGCTGT
    TGGGATGTGG ATCCCGGGCT CCAGGGTGTG GTAACCGACT TGGCAGTCTC TGATGCAGGG
    GAGAAGGTGG AATATAGAAG TTTCTCCGGT TCTTCACAGT CAGAGATTAT ACAAGCAATA
    CAGAATTTAA CGCGTCTCCT CTACAGCCTT CAGGCTGCCT TGACCATCCA GGACAGTCAC
    ATTGAGATCC ACAAACTGGT TCTACAGCAG CGGGAGAGCT TGGCCCCTAG CCACTCCTTC
    CGAGGTGGCC CCTTGCAAGA CCAGGAGAAA TCCCGCTACC TGGAAAAGCA GCGTGAAGAG
    CTGGCCAACA TCCACAAGCT TCAACACCAG TTCCAGCAGG AGCAGCGGCG CTGGCACCGC
    ACGTGCGACC AGCAACAGCG CGAGCAGGAA GCCCAAGAGA GCTGGCTGCA GGCGCGGGAA
    CGTGAGTGCC AGTCCCAGGA GGAATTGTTG CTGCGCCACC GTAGCGAGCT GGACCACCAA
    CTCCAGGAGT ACCAGCAGAG CCTTGAGCGG CTACGGGAGG GCCAGCGGAT GGTGGAGAGG
    GAGAGACAGA AGATGCGTGT CCAGCAGGGT CTGCTGGGCC ACTGCAAGCA TAGTCGCCAG
    CGGAGCCTCC CAGCCGTCTT CTCTCCAGGG AGCAAGGAGG TAACAGAACT TAACCGAGCT
    GAGAGTTTGT GTCATGAAAA TTCATTTTTC ATCAATGAAG CTTTTGGACA CATGTCACTT
    AACACGTCCA ATAAACCAAA CCCATCAGGG GTCCCCTGGG ATGCTCACCC CCTGGAGGGG
    TCTCATTTTG ATTTGGCAAG GACTAGTGAG AGTCCAACAG AACTGAAGAT AGACATTTCT
    CAGCCTCCTG ATGTCAACTC TGAACTCTGG ACAACGGGGC CGGGCCATCA GAGACCTGCT
    CTCCAAGAAA ACAGCAAGGA GTCTTATAAA AATGTTGCAG ATTTGGACTC CTTCCAGAGT
    GAATCGTCAT CTCCCCAGGA CTCCAATCAG CGTGGCCCCC AGCCACAGAC ACTCACAACA
    GAAGCAAAAC TAAGTCTTCC GATGGCGGCT GGACACGGTG GCGATGCTGG AGACGGGGCT
    GAAGAAAACA TTCTTTACCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006517582.1
    CDS1823..5986
    Translation

    Target ORF information:

    RefSeq Version XM_006517519.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006517519.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGATTCTG ACTCGGACTC ACCTTTCAAC TACTCCTGGC CTTCCTTCCC CAAGATGAAG 
    ATTCGGCGCA GGGCATCCAA GAAAGATCGC TCCTTTGATG GCCTCAAAAA ATCCAAGCAT
    GTCCCAGCGT CCCTTGCTGC AGGCCAGCTC TCTGATGTTC TGAACGGGGG TGATGAAGTC
    TACGCTAACT GTATGGTGAT TGACCAGGTT GGCGATTTGG ATATCAACTA TATAAACCTA
    GAGGGACTCT CCACCCATAC GAGTCCCGAG TCTGGGAGGT CCATGCTGGG GCCTCAGGCT
    TGCATGCACA CCCTTCCTCC CGACACCAGT CCCTGCGGGC GCCCACTCAT TGAGAACAGT
    GAGGGGACCC TGGATGCTGC AGCTTCGCAG TCTTTCGTGA CACCATCCAG TTCTCGCACT
    TCCAACTTGA ACCTTTCTTT TGGTCTCCAT GGATTTGAAA AGGAGCAGAG TCATCTCAAG
    AAAAGGAGTT CTAGCCTGGA TGCCCTGGTT GCCGACAGTG AGGGGGAAGG AGGTTCTGAG
    CCCCCCATCT GCTATGCTGT GGGCTCTCAG AGTTCCCCAA GAACTGGACT CCCCAGCGGG
    GATGAACTGG ACTCTTTTGA GACCAACACT GAACCAGACT GTAATATCTC CAGGACCGAA
    TCACTCTCTC TGTCCAGTAC TCTGCATTCA AAGGAGTCCT TGCTTTCTGG GATCCGGTCT
    CGCTCCTACT CCTGCTCATC ACCCAAAATC TCTTCAGGAA AATCCCGGCT AGTGCGCGAC
    TTCACAGTGT GCAGTACCAG CGAAGAACAG AGATCATATA GTTTCCAGGA GCCACCAGGA
    GAAAAAAGGA TTCAGGAAGA AGAATGGGAT GAATACGTCA TTCCTGCCAA ATCCGAGTCT
    GAAAAGTACA AAGTGAGCCG AACTTTCAGC TTCCTCATGA ACAGGATGAC CAGCCCACGG
    AACAAATCTA AGATGAAAAA CAAGGACACT AAAGAAAAAG AAAAAATGAA TCGACATCAG
    TTTGTCCCGG GAACGTTCTC CGGGGTTCTG CAGTGCTCCG GTTGTGATAA AACACTCCTG
    GGGAAAGAGT CACTCCAGTG TGCCAACTGT AAGGCAAACA CACACAAGGG CTGCAAAGAT
    GCTGTGCCTC CGTGTACCAA GAAATTCCAA GAGAAATACA ACAAGAACAA ACCACAGAGC
    ATCCTTGGAA GCTCTTCAGT CAGAGACGTC CCAGCACCTG GGCTCTCCTT GCACCCTTCT
    TCCTCTATGC CCATCGGACT GCCAGCTGGA AGGAAGGAAT TTGCGGCTCA GGTGCATCCA
    CTGTCCAGAA GTGTCCCCGG CACCACTTTG GAAAGCTTCA GGAGGGCAGT GACATCGTTA
    GAGTCTGAGG GTGACAGCTG GAGAAGCAGG TCTCATTCCG ATGAGCTGTT CCAGTCTATG
    GGCTCTTCTC CTTCCACGGA GTCATTCATG ATGGAAGATG TTGTGGATTC TTCTCTGTGG
    ATCGACCTCA GTAGTGATGC TCAGGAGTTT GAAGCAGAGT CTTGGAGTCT CGTGGTGGAC
    CCTTCCTTTT GCAGTAGGCA GGAGAAGGAT GTCATCAAAA GACAAGATGT CATTTTTGAG
    CTGATGCAGA CAGAGGTCCA CCACATCCAG ACTCTGCTCA TCATGTCCGA GGTCTTCAGG
    AAAGGCATGA AGGAGGAACT GCAGCTGGAC CACAGCACGG TGGACAAGAT CTTCCCCTGC
    TTAGATGAGC TGCTGGAGAC TCACCGGCAC TTCTTCTTCA GCATGAAGGA GCGCAGGCAG
    GAGTCCTGCG CTGGCAGTGA CAGGAACTTT GTGATCAACC AAATTGGAGA CATTCTGGTA
    CAACAGTTTT CAGAGGAAAA TGCAAGCAAA ATGAAGAGGA TCTATGGAGA ATTCTGCAGC
    CATCACAAAG AAGCCATGAG TCTCTTTAAA GAGCTGCAGC AGAACAAGAA GTTTCAGAAT
    TTTATTAAGA TCCGAAATAG CAACCTGTTG GCTCGTCGCC GCGGAATCCC GGAGTGCATC
    CTGCTAGTTA CCCAGCGCAT CACAAAGTAC CCAGTCTTGG TGGAAAGGAT CCTGCAGTAC
    ACCAAGGAAA GGACTGAAGA GCACCGGGAC CTGTGCAAAG CTCTCGGCTT AATTAAAGAC
    ATGATCGCAG CCGTGGACCT GAAAGTCAGC GAATACGAGA AGAACCAAAA ATGGCTCGAG
    ATCCTAAATA AAATCGAGAA CAAAACATAC ACAAAGCTCA AAAATGGCCA CGTGTTTAGA
    AAGCAGGCGC TGCTGAGCCA GGAACGGGCT CTCCTGCATG ATGGGCTTGT CTACTGGAAA
    ACCGCTACGG GTCGCTTCAA AGACATCCTG GCTCTGCTAC TAACTGACGT GCTGCTCTTC
    TTACAAGAAA AAGACCAGAA ATACATCTTT GCAGCTGTTG ACCAGAAGCC ATCAGTGATT
    TCCCTCCAGA AGCTGATTGC TAGAGAAGTT GCCAACGAGG AGAGAGGCAT GTTTCTGATC
    AGTGCTTCGT CAGCCGGCCC CGAGATGTAC GAAATCCACA CCAATTCCAA GGAGGAGCGG
    AATAACTGGA TGAGGCGGAT CCAGCAGGCA GTAGAAAGTT GTCCAGAGGA AGAAGGAGGA
    AGGACGAGCG AGTCGGATGA AGAGAGGCGG AAAGCTGAAG CTAGAGTGGC CAAGATTCAG
    CAATGTCAAG AAATACTCAG TAACCAAGAC CAACAGATTT GCACGTACTT GGAAGAAAAG
    CTGCATATCT ACGCTGAACT TGGGGAACTG AGTGGCTTTG AGGATGTCCA CCTAGAACCC
    CATCTGCTCA TCAAACCTGA CCCTGGAGAG CCTCCCCAGG CAGCGTCCCT GCTTGCAGCA
    GCCCTGAGAG AAGCCGAGAG TCTGCAAGTG GCTGTGAAGG CTTCAAAGAT GGGCGATGTA
    AGCCAGTCAT CCGAAGAAAG CCCTGGAGGG ACTGTCCTGA TGGACACACC CAGTACACAG
    GATGTACCAG CGTCACCAAC TGCTTCATTA GTCACAGAAG GGACAGAAGG AAGAGGCTGT
    TGGGATGTGG ATCCCGGGCT CCAGGGTGTG GTAACCGACT TGGCAGTCTC TGATGCAGGG
    GAGAAGGTGG AATATAGAAG TTTCTCCGGT TCTTCACAGT CAGAGATTAT ACAAGCAATA
    CAGAATTTAA CGCGTCTCCT CTACAGCCTT CAGGCTGCCT TGACCATCCA GGACAGTCAC
    ATTGAGATCC ACAAACTGGT TCTACAGCAG CGGGAGAGCT TGGCCCCTAG CCACTCCTTC
    CGAGGTGGCC CCTTGCAAGA CCAGGAGAAA TCCCGCTACC TGGAAAAGCA GCGTGAAGAG
    CTGGCCAACA TCCACAAGCT TCAACACCAG TTCCAGCAGG AGCAGCGGCG CTGGCACCGC
    ACGTGCGACC AGCAACAGCG CGAGCAGGAA GCCCAAGAGA GCTGGCTGCA GGCGCGGGAA
    CGTGAGTGCC AGTCCCAGGA GGAATTGTTG CTGCGCCACC GTAGCGAGCT GGACCACCAA
    CTCCAGGAGT ACCAGCAGAG CCTTGAGCGG CTACGGGAGG GCCAGCGGAT GGTGGAGAGG
    GAGAGACAGA AGATGCGTGT CCAGCAGGGT CTGCTGGGCC ACTGCAAGCA TAGTCGCCAG
    CGGAGCCTCC CAGCCGTCTT CTCTCCAGGG AGCAAGGAGG TAACAGAACT TAACCGAGCT
    GAGAGTTTGT GTCATGAAAA TTCATTTTTC ATCAATGAAG CTTTTGGACA CATGTCACTT
    AACACGTCCA ATAAACCAAA CCCATCAGGG GTCCCCTGGG ATGCTCACCC CCTGGAGGGG
    TCTCATTTTG ATTTGGCAAG GACTAGTGAG AGTCCAACAG AACTGAAGAT AGACATTTCT
    CAGCCTCCTG ATGTCAACTC TGAACTCTGG ACAACGGGGC CGGGCCATCA GAGACCTGCT
    CTCCAAGAAA ACAGCAAGGA GTCTTATAAA AATGTTGCAG ATTTGGACTC CTTCCAGAGT
    GAATCGTCAT CTCCCCAGGA CTCCAATCAG CGTGGCCCCC AGCCACAGAC ACTCACAACA
    GAAGCAAAAC TAAGTCTTCC GATGGCGGCT GGACACGGTG GCGATGCTGG AGACGGGGCT
    GAAGAAAACA TTCTTTACCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu48949
    Clone ID Related Accession (Same CDS sequence) XM_006517521.3 , XM_006517521.4
    Accession Version XM_006517521.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 28 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006517521.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGGCGGC GACGAGCCCG GCTGGCCAGA GATGCAGGAG CCATGGAGTT GAGCTGCAGT 
    GAAGTGCCTC TTTATGGACA GAAGACGGTC TATGCAAAGT TTGGCAAAAA CGTGTATCTA
    CCCGAAGATG CGGAGTTTTA CTTTGTTTAT GGCGGGTCGC ATCAGAGGCA CGTGGTGATT
    GCTGACCGTG TCCAGGACAA TGTCCTCCAA TCTAGCATTC CAGGCCACTG GCTTCAGGAG
    ACGGTGACGG TATCTGTGTG CCTCTGCTCG GAAGGGTACT CCCCAGTGAC CATGGGCTCT
    GGCTCAGTGA CCTACGTGGA CAACATGGCC TGCAGGCTGG CTCGTCTGCT GGTCACTCAG
    GCTGACCGAC TCACGGCCTG CAGTCACCAG ACGCTGCTGA CCCCGTTTGC CCTGACAGTG
    GAAGCGCTGC CTGCCTTGGA TGAGGAGCTC GTGCTGGCTC TGACCCAACT GGAGCTGCCT
    TTGGGTTGGA CAGTCCTGGG AAATTCCTCA CTTGAAGTAT CTCTACACAG AGAGTCTCTC
    CTGCATCTGG CTGTGAGATG GGCCCTCCCT AAGCTATTCC ACTTCCTCTT GTGTCTCCCT
    GGAGGAGTCA AGGCCTTGAA GCTACCTAAT GAAGAGGCCA CCACACCATT AGACTTAGCT
    TTGCAAGGTG GACACTCCAC ACTGGTAGAA GACATCACAA ATTTTCAGGG TAGCCATTCC
    CCAGGCTTCT CCCGACTGCG TCTTAACGAA GAGGCCACTC TGCAGTTTGT TCATTCATCG
    GAAACATTGA CCCTGACCGT TAACCACACA GCTGAGCATC TCTTGGAAGC AGACATCAAA
    CTCTTCCGGA AATATTTCTG GGACAGAGCC TTTCTTGTCA AGGCTCTTGA GCAAGAAGCC
    AAGACTGAGA AAGCAACCAT GCCTTCTGGA GCTGCAGAAA CTGAAGAAGA GGTTAGGAAT
    TTGGAGTCTG GCAGATCACC ATCGGAGGAG GAGGAGGATG CAAAGAGCAT CAAAAGCCAG
    GTGGATGGGC CGAGTGAACA CGAAGACCAG GACCGCCTAC CTCTGGATCG CTCCTTTGAT
    GGCCTCAAAA AATCCAAGCA TGTCCCAGCG TCCCTTGCTG CAGGCCAGCT CTCTGATGTT
    CTGAACGGGG GTGATGAAGT CTACGCTAAC TGTATGGTGA TTGACCAGGT TGGCGATTTG
    GATATCAACT ATATAAACCT AGAGGGACTC TCCACCCATA CGAGTCCCGA GTCTGGGAGG
    TCCATGCTGG GGCCTCAGGC TTGCATGCAC ACCCTTCCTC CCGACACCAG TCCCTGCGGG
    CGCCCACTCA TTGAGAACAG TGAGGGGACC CTGGATGCTG CAGCTTCGCA GTCTTTCGTG
    ACACCATCCA GTTCTCGCAC TTCCAACTTG AACCTTTCTT TTGGTCTCCA TGGATTTGAA
    AAGGAGCAGA GTCATCTCAA GAAAAGGAGT TCTAGCCTGG ATGCCCTGGT TGCCGACAGT
    GAGGGGGAAG GAGGTTCTGA GCCCCCCATC TGCTATGCTG TGGGCTCTCA GAGTTCCCCA
    AGAACTGGAC TCCCCAGCGG GGATGAACTG GACTCTTTTG AGACCAACAC TGAACCAGAC
    TGTAATATCT CCAGGACCGA ATCACTCTCT CTGTCCAGTA CTCTGCATTC AAAGGAGTCC
    TTGCTTTCTG GGATCCGGTC TCGCTCCTAC TCCTGCTCAT CACCCAAAAT CTCTTCAGGA
    AAATCCCGGC TAGTGCGCGA CTTCACAGTG TGCAGTACCA GCGAAGAACA GAGATCATAT
    AGTTTCCAGG AGCCACCAGG AGAAAAAAGG ATTCAGGAAG AAGAATGGGA TGAATACGTC
    ATTCCTGCCA AATCCGAGTC TGAAAAGTAC AAAGTGAGCC GAACTTTCAG CTTCCTCATG
    AACAGGATGA CCAGCCCACG GAACAAATCT AAGATGAAAA ACAAGGACAC TAAAGAAAAA
    GAAAAAATGA ATCGACATCA GTTTGTCCCG GGAACGTTCT CCGGGGTTCT GCAGTGCTCC
    GGTTGTGATA AAACACTCCT GGGGAAAGAG TCACTCCAGT GTGCCAACTG TAAGGCAAAC
    ACACACAAGG GCTGCAAAGA TGCTGTGCCT CCGTGTACCA AGAAATTCCA AGAGAAATAC
    AACAAGAACA AACCACAGAG CATCCTTGGA AGCTCTTCAG TCAGAGACGT CCCAGCACCT
    GGGCTCTCCT TGCACCCTTC TTCCTCTATG CCCATCGGAC TGCCAGCTGG AAGGAAGGAA
    TTTGCGGCTC AGGTGCATCC ACTGTCCAGA AGTGTCCCCG GCACCACTTT GGAAAGCTTC
    AGGAGGGCAG TGACATCGTT AGAGTCTGAG GGTGACAGCT GGAGAAGCAG GTCTCATTCC
    GATGAGCTGT TCCAGTCTAT GGGCTCTTCT CCTTCCACGG AGTCATTCAT GATGGAAGAT
    GTTGTGGATT CTTCTCTGTG GATCGACCTC AGTAGTGATG CTCAGGAGTT TGAAGCAGAG
    TCTTGGAGTC TCGTGGTGGA CCCTTCCTTT TGCAGTAGGC AGGAGAAGGA TGTCATCAAA
    AGACAAGATG TCATTTTTGA GCTGATGCAG ACAGAGGTCC ACCACATCCA GACTCTGCTC
    ATCATGTCCG AGGTCTTCAG GAAAGGCATG AAGGAGGAAC TGCAGCTGGA CCACAGCACG
    GTGGACAAGA TCTTCCCCTG CTTAGATGAG CTGCTGGAGA CTCACCGGCA CTTCTTCTTC
    AGCATGAAGG AGCGCAGGCA GGAGTCCTGC GCTGGCAGTG ACAGGAACTT TGTGATCAAC
    CAAATTGGAG ACATTCTGGT ACAACAGTTT TCAGAGGAAA ATGCAAGCAA AATGAAGAGG
    ATCTATGGAG AATTCTGCAG CCATCACAAA GAAGCCATGA GTCTCTTTAA AGAGCTGCAG
    CAGAACAAGA AGTTTCAGAA TTTTATTAAG ATCCGAAATA GCAACCTGTT GGCTCGTCGC
    CGCGGAATCC CGGAGTGCAT CCTGCTAGTT ACCCAGCGCA TCACAAAGTA CCCAGTCTTG
    GTGGAAAGGA TCCTGCAGTA CACCAAGGAA AGGACTGAAG AGCACCGGGA CCTGTGCAAA
    GCTCTCGGCT TAATTAAAGA CATGATCGCA GCCGTGGACC TGAAAGTCAG CGAATACGAG
    AAGAACCAAA AATGGCTCGA GATCCTAAAT AAAATCGAGA ACAAAACATA CACAAAGCTC
    AAAAATGGCC ACGTGTTTAG AAAGCAGGCG CTGCTGAGCC AGGAACGGGC TCTCCTGCAT
    GATGGGCTTG TCTACTGGAA AACCGCTACG GGTCGCTTCA AAGACATCCT GGCTCTGCTA
    CTAACTGACG TGCTGCTCTT CTTACAAGAA AAAGACCAGA AATACATCTT TGCAGCTGTT
    GACCAGAAGC CATCAGTGAT TTCCCTCCAG AAGCTGATTG CTAGAGAAGT TGCCAACGAG
    GAGAGAGGCA TGTTTCTGAT CAGTGCTTCG TCAGCCGGCC CCGAGATGTA CGAAATCCAC
    ACCAATTCCA AGGAGGAGCG GAATAACTGG ATGAGGCGGA TCCAGCAGGC AGTAGAAAGT
    TGTCCAGAGG AAGAAGGAGG AAGGACGAGC GAGTCGGATG AAGAGAGGCG GAAAGCTGAA
    GCTAGAGTGG CCAAGATTCA GCAATGTCAA GAAATACTCA GTAACCAAGA CCAACAGATT
    TGCACGTACT TGGAAGAAAA GCTGCATATC TACGCTGAAC TTGGGGAACT GAGTGGCTTT
    GAGGATGTCC ACCTAGAACC CCATCTGCTC ATCAAACCTG ACCCTGGAGA GCCTCCCCAG
    GCAGCGTCCC TGCTTGCAGC AGCCCTGAGA GAAGCCGAGA GTCTGCAAGT GGCTGTGAAG
    GCTTCAAAGA TGGGCGATGT AAGCCAGTCA TCCGAAGAAA GCCCTGGAGG GACTGTCCTG
    ATGGACACAC CCAGTACACA GGATGTACCA GCGTCACCAA CTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006517584.1
    CDS322..4329
    Translation

    Target ORF information:

    RefSeq Version XM_006517521.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006517521.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGGCGGC GACGAGCCCG GCTGGCCAGA GATGCAGGAG CCATGGAGTT GAGCTGCAGT 
    GAAGTGCCTC TTTATGGACA GAAGACGGTC TATGCAAAGT TTGGCAAAAA CGTGTATCTA
    CCCGAAGATG CGGAGTTTTA CTTTGTTTAT GGCGGGTCGC ATCAGAGGCA CGTGGTGATT
    GCTGACCGTG TCCAGGACAA TGTCCTCCAA TCTAGCATTC CAGGCCACTG GCTTCAGGAG
    ACGGTGACGG TATCTGTGTG CCTCTGCTCG GAAGGGTACT CCCCAGTGAC CATGGGCTCT
    GGCTCAGTGA CCTACGTGGA CAACATGGCC TGCAGGCTGG CTCGTCTGCT GGTCACTCAG
    GCTGACCGAC TCACGGCCTG CAGTCACCAG ACGCTGCTGA CCCCGTTTGC CCTGACAGTG
    GAAGCGCTGC CTGCCTTGGA TGAGGAGCTC GTGCTGGCTC TGACCCAACT GGAGCTGCCT
    TTGGGTTGGA CAGTCCTGGG AAATTCCTCA CTTGAAGTAT CTCTACACAG AGAGTCTCTC
    CTGCATCTGG CTGTGAGATG GGCCCTCCCT AAGCTATTCC ACTTCCTCTT GTGTCTCCCT
    GGAGGAGTCA AGGCCTTGAA GCTACCTAAT GAAGAGGCCA CCACACCATT AGACTTAGCT
    TTGCAAGGTG GACACTCCAC ACTGGTAGAA GACATCACAA ATTTTCAGGG TAGCCATTCC
    CCAGGCTTCT CCCGACTGCG TCTTAACGAA GAGGCCACTC TGCAGTTTGT TCATTCATCG
    GAAACATTGA CCCTGACCGT TAACCACACA GCTGAGCATC TCTTGGAAGC AGACATCAAA
    CTCTTCCGGA AATATTTCTG GGACAGAGCC TTTCTTGTCA AGGCTCTTGA GCAAGAAGCC
    AAGACTGAGA AAGCAACCAT GCCTTCTGGA GCTGCAGAAA CTGAAGAAGA GGTTAGGAAT
    TTGGAGTCTG GCAGATCACC ATCGGAGGAG GAGGAGGATG CAAAGAGCAT CAAAAGCCAG
    GTGGATGGGC CGAGTGAACA CGAAGACCAG GACCGCCTAC CTCTGGATCG CTCCTTTGAT
    GGCCTCAAAA AATCCAAGCA TGTCCCAGCG TCCCTTGCTG CAGGCCAGCT CTCTGATGTT
    CTGAACGGGG GTGATGAAGT CTACGCTAAC TGTATGGTGA TTGACCAGGT TGGCGATTTG
    GATATCAACT ATATAAACCT AGAGGGACTC TCCACCCATA CGAGTCCCGA GTCTGGGAGG
    TCCATGCTGG GGCCTCAGGC TTGCATGCAC ACCCTTCCTC CCGACACCAG TCCCTGCGGG
    CGCCCACTCA TTGAGAACAG TGAGGGGACC CTGGATGCTG CAGCTTCGCA GTCTTTCGTG
    ACACCATCCA GTTCTCGCAC TTCCAACTTG AACCTTTCTT TTGGTCTCCA TGGATTTGAA
    AAGGAGCAGA GTCATCTCAA GAAAAGGAGT TCTAGCCTGG ATGCCCTGGT TGCCGACAGT
    GAGGGGGAAG GAGGTTCTGA GCCCCCCATC TGCTATGCTG TGGGCTCTCA GAGTTCCCCA
    AGAACTGGAC TCCCCAGCGG GGATGAACTG GACTCTTTTG AGACCAACAC TGAACCAGAC
    TGTAATATCT CCAGGACCGA ATCACTCTCT CTGTCCAGTA CTCTGCATTC AAAGGAGTCC
    TTGCTTTCTG GGATCCGGTC TCGCTCCTAC TCCTGCTCAT CACCCAAAAT CTCTTCAGGA
    AAATCCCGGC TAGTGCGCGA CTTCACAGTG TGCAGTACCA GCGAAGAACA GAGATCATAT
    AGTTTCCAGG AGCCACCAGG AGAAAAAAGG ATTCAGGAAG AAGAATGGGA TGAATACGTC
    ATTCCTGCCA AATCCGAGTC TGAAAAGTAC AAAGTGAGCC GAACTTTCAG CTTCCTCATG
    AACAGGATGA CCAGCCCACG GAACAAATCT AAGATGAAAA ACAAGGACAC TAAAGAAAAA
    GAAAAAATGA ATCGACATCA GTTTGTCCCG GGAACGTTCT CCGGGGTTCT GCAGTGCTCC
    GGTTGTGATA AAACACTCCT GGGGAAAGAG TCACTCCAGT GTGCCAACTG TAAGGCAAAC
    ACACACAAGG GCTGCAAAGA TGCTGTGCCT CCGTGTACCA AGAAATTCCA AGAGAAATAC
    AACAAGAACA AACCACAGAG CATCCTTGGA AGCTCTTCAG TCAGAGACGT CCCAGCACCT
    GGGCTCTCCT TGCACCCTTC TTCCTCTATG CCCATCGGAC TGCCAGCTGG AAGGAAGGAA
    TTTGCGGCTC AGGTGCATCC ACTGTCCAGA AGTGTCCCCG GCACCACTTT GGAAAGCTTC
    AGGAGGGCAG TGACATCGTT AGAGTCTGAG GGTGACAGCT GGAGAAGCAG GTCTCATTCC
    GATGAGCTGT TCCAGTCTAT GGGCTCTTCT CCTTCCACGG AGTCATTCAT GATGGAAGAT
    GTTGTGGATT CTTCTCTGTG GATCGACCTC AGTAGTGATG CTCAGGAGTT TGAAGCAGAG
    TCTTGGAGTC TCGTGGTGGA CCCTTCCTTT TGCAGTAGGC AGGAGAAGGA TGTCATCAAA
    AGACAAGATG TCATTTTTGA GCTGATGCAG ACAGAGGTCC ACCACATCCA GACTCTGCTC
    ATCATGTCCG AGGTCTTCAG GAAAGGCATG AAGGAGGAAC TGCAGCTGGA CCACAGCACG
    GTGGACAAGA TCTTCCCCTG CTTAGATGAG CTGCTGGAGA CTCACCGGCA CTTCTTCTTC
    AGCATGAAGG AGCGCAGGCA GGAGTCCTGC GCTGGCAGTG ACAGGAACTT TGTGATCAAC
    CAAATTGGAG ACATTCTGGT ACAACAGTTT TCAGAGGAAA ATGCAAGCAA AATGAAGAGG
    ATCTATGGAG AATTCTGCAG CCATCACAAA GAAGCCATGA GTCTCTTTAA AGAGCTGCAG
    CAGAACAAGA AGTTTCAGAA TTTTATTAAG ATCCGAAATA GCAACCTGTT GGCTCGTCGC
    CGCGGAATCC CGGAGTGCAT CCTGCTAGTT ACCCAGCGCA TCACAAAGTA CCCAGTCTTG
    GTGGAAAGGA TCCTGCAGTA CACCAAGGAA AGGACTGAAG AGCACCGGGA CCTGTGCAAA
    GCTCTCGGCT TAATTAAAGA CATGATCGCA GCCGTGGACC TGAAAGTCAG CGAATACGAG
    AAGAACCAAA AATGGCTCGA GATCCTAAAT AAAATCGAGA ACAAAACATA CACAAAGCTC
    AAAAATGGCC ACGTGTTTAG AAAGCAGGCG CTGCTGAGCC AGGAACGGGC TCTCCTGCAT
    GATGGGCTTG TCTACTGGAA AACCGCTACG GGTCGCTTCA AAGACATCCT GGCTCTGCTA
    CTAACTGACG TGCTGCTCTT CTTACAAGAA AAAGACCAGA AATACATCTT TGCAGCTGTT
    GACCAGAAGC CATCAGTGAT TTCCCTCCAG AAGCTGATTG CTAGAGAAGT TGCCAACGAG
    GAGAGAGGCA TGTTTCTGAT CAGTGCTTCG TCAGCCGGCC CCGAGATGTA CGAAATCCAC
    ACCAATTCCA AGGAGGAGCG GAATAACTGG ATGAGGCGGA TCCAGCAGGC AGTAGAAAGT
    TGTCCAGAGG AAGAAGGAGG AAGGACGAGC GAGTCGGATG AAGAGAGGCG GAAAGCTGAA
    GCTAGAGTGG CCAAGATTCA GCAATGTCAA GAAATACTCA GTAACCAAGA CCAACAGATT
    TGCACGTACT TGGAAGAAAA GCTGCATATC TACGCTGAAC TTGGGGAACT GAGTGGCTTT
    GAGGATGTCC ACCTAGAACC CCATCTGCTC ATCAAACCTG ACCCTGGAGA GCCTCCCCAG
    GCAGCGTCCC TGCTTGCAGC AGCCCTGAGA GAAGCCGAGA GTCTGCAAGT GGCTGTGAAG
    GCTTCAAAGA TGGGCGATGT AAGCCAGTCA TCCGAAGAAA GCCCTGGAGG GACTGTCCTG
    ATGGACACAC CCAGTACACA GGATGTACCA GCGTCACCAA CTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu78410
    Clone ID Related Accession (Same CDS sequence) XM_017315362.1 , XM_017315362.2
    Accession Version XM_017315362.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4155bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 28 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTTTTTGA CTTCTGGAGC CTGTCGGCAC TGTACAGCCG TGATGTCCCT GAGGTACCCT 
    GTGTTCCTGC ATGAAGATCG CTCCTTTGAT GGCCTCAAAA AATCCAAGCA TGTCCCAGCG
    TCCCTTGCTG CAGGCCAGCT CTCTGATGTT CTGAACGGGG GTGATGAAGT CTACGCTAAC
    TGTATGGTGA TTGACCAGGT TGGCGATTTG GATATCAACT ATATAAACCT AGAGGGACTC
    TCCACCCATA CGAGTCCCGA GTCTGGGAGG TCCATGCTGG GGCCTCAGGC TTGCATGCAC
    ACCCTTCCTC CCGACACCAG TCCCTGCGGG CGCCCACTCA TTGAGAACAG TGAGGGGACC
    CTGGATGCTG CAGCTTCGCA GTCTTTCGTG ACACCATCCA GTTCTCGCAC TTCCAACTTG
    AACCTTTCTT TTGGTCTCCA TGGATTTGAA AAGGAGCAGA GTCATCTCAA GAAAAGGAGT
    TCTAGCCTGG ATGCCCTGGT TGCCGACAGT GAGGGGGAAG GAGGTTCTGA GCCCCCCATC
    TGCTATGCTG TGGGCTCTCA GAGTTCCCCA AGAACTGGAC TCCCCAGCGG GGATGAACTG
    GACTCTTTTG AGACCAACAC TGAACCAGAC TGTAATATCT CCAGGACCGA ATCACTCTCT
    CTGTCCAGTA CTCTGCATTC AAAGGAGTCC TTGCTTTCTG GGATCCGGTC TCGCTCCTAC
    TCCTGCTCAT CACCCAAAAT CTCTTCAGGA AAATCCCGGC TAGTGCGCGA CTTCACAGTG
    TGCAGTACCA GCGAAGAACA GAGATCATAT AGTTTCCAGG AGCCACCAGG AGAAAAAAGG
    ATTCAGGAAG AAGAATGGGA TGAATACGTC ATTCCTGCCA AATCCGAGTC TGAAAAGTAC
    AAAGTGAGCC GAACTTTCAG CTTCCTCATG AACAGGATGA CCAGCCCACG GAACAAATCT
    AAGATGAAAA ACAAGGACAC TAAAGAAAAA GAAAAAATGA ATCGACATCA GTTTGTCCCG
    GGAACGTTCT CCGGGGTTCT GCAGTGCTCC GGTTGTGATA AAACACTCCT GGGGAAAGAG
    TCACTCCAGT GTGCCAACTG TAAGGCAAAC ACACACAAGG GCTGCAAAGA TGCTGTGCCT
    CCGTGTACCA AGAAATTCCA AGAGAAATAC AACAAGAACA AACCACAGAG CATCCTTGGA
    AGCTCTTCAG TCAGAGACGT CCCAGCACCT GGGCTCTCCT TGCACCCTTC TTCCTCTATG
    CCCATCGGAC TGCCAGCTGG AAGGAAGGAA TTTGCGGCTC AGGTGCATCC ACTGTCCAGA
    AGTGTCCCCG GCACCACTTT GGAAAGCTTC AGGAGGGCAG TGACATCGTT AGAGTCTGAG
    GGTGACAGCT GGAGAAGCAG GTCTCATTCC GATGAGCTGT TCCAGTCTAT GGGCTCTTCT
    CCTTCCACGG AGTCATTCAT GATGGAAGAT GTTGTGGATT CTTCTCTGTG GATCGACCTC
    AGTAGTGATG CTCAGGAGTT TGAAGCAGAG TCTTGGAGTC TCGTGGTGGA CCCTTCCTTT
    TGCAGTAGGC AGGAGAAGGA TGTCATCAAA AGACAAGATG TCATTTTTGA GCTGATGCAG
    ACAGAGGTCC ACCACATCCA GACTCTGCTC ATCATGTCCG AGGTCTTCAG GAAAGGCATG
    AAGGAGGAAC TGCAGCTGGA CCACAGCACG GTGGACAAGA TCTTCCCCTG CTTAGATGAG
    CTGCTGGAGA CTCACCGGCA CTTCTTCTTC AGCATGAAGG AGCGCAGGCA GGAGTCCTGC
    GCTGGCAGTG ACAGGAACTT TGTGATCAAC CAAATTGGAG ACATTCTGGT ACAACAGTTT
    TCAGAGGAAA ATGCAAGCAA AATGAAGAGG ATCTATGGAG AATTCTGCAG CCATCACAAA
    GAAGCCATGA GTCTCTTTAA AGAGCTGCAG CAGAACAAGA AGTTTCAGAA TTTTATTAAG
    ATCCGAAATA GCAACCTGTT GGCTCGTCGC CGCGGAATCC CGGAGTGCAT CCTGCTAGTT
    ACCCAGCGCA TCACAAAGTA CCCAGTCTTG GTGGAAAGGA TCCTGCAGTA CACCAAGGAA
    AGGACTGAAG AGCACCGGGA CCTGTGCAAA GCTCTCGGCT TAATTAAAGA CATGATCGCA
    GCCGTGGACC TGAAAGTCAG CGAATACGAG AAGAACCAAA AATGGCTCGA GATCCTAAAT
    AAAATCGAGA ACAAAACATA CACAAAGCTC AAAAATGGCC ACGTGTTTAG AAAGCAGGCG
    CTGCTGAGCC AGGAACGGGC TCTCCTGCAT GATGGGCTTG TCTACTGGAA AACCGCTACG
    GGTCGCTTCA AAGACATCCT GGCTCTGCTA CTAACTGACG TGCTGCTCTT CTTACAAGAA
    AAAGACCAGA AATACATCTT TGCAGCTGTT GACCAGAAGC CATCAGTGAT TTCCCTCCAG
    AAGCTGATTG CTAGAGAAGT TGCCAACGAG GAGAGAGGCA TGTTTCTGAT CAGTGCTTCG
    TCAGCCGGCC CCGAGATGTA CGAAATCCAC ACCAATTCCA AGGAGGAGCG GAATAACTGG
    ATGAGGCGGA TCCAGCAGGC AGTAGAAAGT TGTCCAGAGG AAGAAGGAGG AAGGACGAGC
    GAGTCGGATG AAGAGAGGCG GAAAGCTGAA GCTAGAGTGG CCAAGATTCA GCAATGTCAA
    GAAATACTCA GTAACCAAGA CCAACAGATT TGCACGTACT TGGAAGAAAA GCTGCATATC
    TACGCTGAAC TTGGGGAACT GAGTGGCTTT GAGGATGTCC ACCTAGAACC CCATCTGCTC
    ATCAAACCTG ACCCTGGAGA GCCTCCCCAG GCAGCGTCCC TGCTTGCAGC AGCCCTGAGA
    GAAGCCGAGA GTCTGCAAGT GGCTGTGAAG GCTTCAAAGA TGGGCGATGT AAGCCAGTCA
    TCCGAAGAAA GCCCTGGAGG GACTGTCCTG ATGGACACAC CCAGTACACA GGATGTACCA
    GCGTCACCAA CTGCTTCATT AGTCACAGAA GGGACAGAAG GAAGAGGCTG TTGGGATGTG
    GATCCCGGGC TCCAGGGTGT GGTAACCGAC TTGGCAGTCT CTGATGCAGG GGAGAAGGTG
    GAATATAGAA GTTTCTCCGG TTCTTCACAG TCAGAGATTA TACAAGCAAT ACAGAATTTA
    ACGCGTCTCC TCTACAGCCT TCAGGCTGCC TTGACCATCC AGGACAGTCA CATTGAGATC
    CACAAACTGG TTCTACAGCA GCGGGAGAGC TTGGCCCCTA GCCACTCCTT CCGAGGTGGC
    CCCTTGCAAG ACCAGGAGAA ATCCCGCTAC CTGGAAAAGC AGCGTGAAGA GCTGGCCAAC
    ATCCACAAGC TTCAACACCA GTTCCAGCAG GAGCAGCGGC GCTGGCACCG CACGTGCGAC
    CAGCAACAGC GCGAGCAGGA AGCCCAAGAG AGCTGGCTGC AGGCGCGGGA ACGTGAGTGC
    CAGTCCCAGG AGGAATTGTT GCTGCGCCAC CGTAGCGAGC TGGACCACCA ACTCCAGGAG
    TACCAGCAGA GCCTTGAGCG GCTACGGGAG GGCCAGCGGA TGGTGGAGAG GGAGAGACAG
    AAGATGCGTG TCCAGCAGGG TCTGCTGGGC CACTGCAAGC ATAGTCGCCA GCGGAGCCTC
    CCAGCCGTCT TCTCTCCAGG GAGCAAGGAG GTAACAGAAC TTAACCGAGC TGAGAGTTTG
    TGTCATGAAA ATTCATTTTT CATCAATGAA GCTTTTGGAC ACATGTCACT TAACACGTCC
    AATAAACCAA ACCCATCAGG GGTCCCCTGG GATGCTCACC CCCTGGAGGG GTCTCATTTT
    GATTTGGCAA GGACTAGTGA GAGTCCAACA GAACTGAAGA TAGACATTTC TCAGCCTCCT
    GATGTCAACT CTGAACTCTG GACAACGGGG CCGGGCCATC AGAGACCTGC TCTCCAAGAA
    AACAGCAAGG AGTCTTATAA AAATGTTGCA GATTTGGACT CCTTCCAGAG TGAATCGTCA
    TCTCCCCAGG ACTCCAATCA GCGTGGCCCC CAGCCACAGA CACTCACAAC AGAAGCAAAA
    CTAAGTCTTC CGATGGCGGC TGGACACGGT GGCGATGCTG GAGACGGGGC TGAAGAAAAC
    ATTCTTTACC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170851.1
    CDS91..4245
    Translation

    Target ORF information:

    RefSeq Version XM_017315362.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017315362.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTTTTTGA CTTCTGGAGC CTGTCGGCAC TGTACAGCCG TGATGTCCCT GAGGTACCCT 
    GTGTTCCTGC ATGAAGATCG CTCCTTTGAT GGCCTCAAAA AATCCAAGCA TGTCCCAGCG
    TCCCTTGCTG CAGGCCAGCT CTCTGATGTT CTGAACGGGG GTGATGAAGT CTACGCTAAC
    TGTATGGTGA TTGACCAGGT TGGCGATTTG GATATCAACT ATATAAACCT AGAGGGACTC
    TCCACCCATA CGAGTCCCGA GTCTGGGAGG TCCATGCTGG GGCCTCAGGC TTGCATGCAC
    ACCCTTCCTC CCGACACCAG TCCCTGCGGG CGCCCACTCA TTGAGAACAG TGAGGGGACC
    CTGGATGCTG CAGCTTCGCA GTCTTTCGTG ACACCATCCA GTTCTCGCAC TTCCAACTTG
    AACCTTTCTT TTGGTCTCCA TGGATTTGAA AAGGAGCAGA GTCATCTCAA GAAAAGGAGT
    TCTAGCCTGG ATGCCCTGGT TGCCGACAGT GAGGGGGAAG GAGGTTCTGA GCCCCCCATC
    TGCTATGCTG TGGGCTCTCA GAGTTCCCCA AGAACTGGAC TCCCCAGCGG GGATGAACTG
    GACTCTTTTG AGACCAACAC TGAACCAGAC TGTAATATCT CCAGGACCGA ATCACTCTCT
    CTGTCCAGTA CTCTGCATTC AAAGGAGTCC TTGCTTTCTG GGATCCGGTC TCGCTCCTAC
    TCCTGCTCAT CACCCAAAAT CTCTTCAGGA AAATCCCGGC TAGTGCGCGA CTTCACAGTG
    TGCAGTACCA GCGAAGAACA GAGATCATAT AGTTTCCAGG AGCCACCAGG AGAAAAAAGG
    ATTCAGGAAG AAGAATGGGA TGAATACGTC ATTCCTGCCA AATCCGAGTC TGAAAAGTAC
    AAAGTGAGCC GAACTTTCAG CTTCCTCATG AACAGGATGA CCAGCCCACG GAACAAATCT
    AAGATGAAAA ACAAGGACAC TAAAGAAAAA GAAAAAATGA ATCGACATCA GTTTGTCCCG
    GGAACGTTCT CCGGGGTTCT GCAGTGCTCC GGTTGTGATA AAACACTCCT GGGGAAAGAG
    TCACTCCAGT GTGCCAACTG TAAGGCAAAC ACACACAAGG GCTGCAAAGA TGCTGTGCCT
    CCGTGTACCA AGAAATTCCA AGAGAAATAC AACAAGAACA AACCACAGAG CATCCTTGGA
    AGCTCTTCAG TCAGAGACGT CCCAGCACCT GGGCTCTCCT TGCACCCTTC TTCCTCTATG
    CCCATCGGAC TGCCAGCTGG AAGGAAGGAA TTTGCGGCTC AGGTGCATCC ACTGTCCAGA
    AGTGTCCCCG GCACCACTTT GGAAAGCTTC AGGAGGGCAG TGACATCGTT AGAGTCTGAG
    GGTGACAGCT GGAGAAGCAG GTCTCATTCC GATGAGCTGT TCCAGTCTAT GGGCTCTTCT
    CCTTCCACGG AGTCATTCAT GATGGAAGAT GTTGTGGATT CTTCTCTGTG GATCGACCTC
    AGTAGTGATG CTCAGGAGTT TGAAGCAGAG TCTTGGAGTC TCGTGGTGGA CCCTTCCTTT
    TGCAGTAGGC AGGAGAAGGA TGTCATCAAA AGACAAGATG TCATTTTTGA GCTGATGCAG
    ACAGAGGTCC ACCACATCCA GACTCTGCTC ATCATGTCCG AGGTCTTCAG GAAAGGCATG
    AAGGAGGAAC TGCAGCTGGA CCACAGCACG GTGGACAAGA TCTTCCCCTG CTTAGATGAG
    CTGCTGGAGA CTCACCGGCA CTTCTTCTTC AGCATGAAGG AGCGCAGGCA GGAGTCCTGC
    GCTGGCAGTG ACAGGAACTT TGTGATCAAC CAAATTGGAG ACATTCTGGT ACAACAGTTT
    TCAGAGGAAA ATGCAAGCAA AATGAAGAGG ATCTATGGAG AATTCTGCAG CCATCACAAA
    GAAGCCATGA GTCTCTTTAA AGAGCTGCAG CAGAACAAGA AGTTTCAGAA TTTTATTAAG
    ATCCGAAATA GCAACCTGTT GGCTCGTCGC CGCGGAATCC CGGAGTGCAT CCTGCTAGTT
    ACCCAGCGCA TCACAAAGTA CCCAGTCTTG GTGGAAAGGA TCCTGCAGTA CACCAAGGAA
    AGGACTGAAG AGCACCGGGA CCTGTGCAAA GCTCTCGGCT TAATTAAAGA CATGATCGCA
    GCCGTGGACC TGAAAGTCAG CGAATACGAG AAGAACCAAA AATGGCTCGA GATCCTAAAT
    AAAATCGAGA ACAAAACATA CACAAAGCTC AAAAATGGCC ACGTGTTTAG AAAGCAGGCG
    CTGCTGAGCC AGGAACGGGC TCTCCTGCAT GATGGGCTTG TCTACTGGAA AACCGCTACG
    GGTCGCTTCA AAGACATCCT GGCTCTGCTA CTAACTGACG TGCTGCTCTT CTTACAAGAA
    AAAGACCAGA AATACATCTT TGCAGCTGTT GACCAGAAGC CATCAGTGAT TTCCCTCCAG
    AAGCTGATTG CTAGAGAAGT TGCCAACGAG GAGAGAGGCA TGTTTCTGAT CAGTGCTTCG
    TCAGCCGGCC CCGAGATGTA CGAAATCCAC ACCAATTCCA AGGAGGAGCG GAATAACTGG
    ATGAGGCGGA TCCAGCAGGC AGTAGAAAGT TGTCCAGAGG AAGAAGGAGG AAGGACGAGC
    GAGTCGGATG AAGAGAGGCG GAAAGCTGAA GCTAGAGTGG CCAAGATTCA GCAATGTCAA
    GAAATACTCA GTAACCAAGA CCAACAGATT TGCACGTACT TGGAAGAAAA GCTGCATATC
    TACGCTGAAC TTGGGGAACT GAGTGGCTTT GAGGATGTCC ACCTAGAACC CCATCTGCTC
    ATCAAACCTG ACCCTGGAGA GCCTCCCCAG GCAGCGTCCC TGCTTGCAGC AGCCCTGAGA
    GAAGCCGAGA GTCTGCAAGT GGCTGTGAAG GCTTCAAAGA TGGGCGATGT AAGCCAGTCA
    TCCGAAGAAA GCCCTGGAGG GACTGTCCTG ATGGACACAC CCAGTACACA GGATGTACCA
    GCGTCACCAA CTGCTTCATT AGTCACAGAA GGGACAGAAG GAAGAGGCTG TTGGGATGTG
    GATCCCGGGC TCCAGGGTGT GGTAACCGAC TTGGCAGTCT CTGATGCAGG GGAGAAGGTG
    GAATATAGAA GTTTCTCCGG TTCTTCACAG TCAGAGATTA TACAAGCAAT ACAGAATTTA
    ACGCGTCTCC TCTACAGCCT TCAGGCTGCC TTGACCATCC AGGACAGTCA CATTGAGATC
    CACAAACTGG TTCTACAGCA GCGGGAGAGC TTGGCCCCTA GCCACTCCTT CCGAGGTGGC
    CCCTTGCAAG ACCAGGAGAA ATCCCGCTAC CTGGAAAAGC AGCGTGAAGA GCTGGCCAAC
    ATCCACAAGC TTCAACACCA GTTCCAGCAG GAGCAGCGGC GCTGGCACCG CACGTGCGAC
    CAGCAACAGC GCGAGCAGGA AGCCCAAGAG AGCTGGCTGC AGGCGCGGGA ACGTGAGTGC
    CAGTCCCAGG AGGAATTGTT GCTGCGCCAC CGTAGCGAGC TGGACCACCA ACTCCAGGAG
    TACCAGCAGA GCCTTGAGCG GCTACGGGAG GGCCAGCGGA TGGTGGAGAG GGAGAGACAG
    AAGATGCGTG TCCAGCAGGG TCTGCTGGGC CACTGCAAGC ATAGTCGCCA GCGGAGCCTC
    CCAGCCGTCT TCTCTCCAGG GAGCAAGGAG GTAACAGAAC TTAACCGAGC TGAGAGTTTG
    TGTCATGAAA ATTCATTTTT CATCAATGAA GCTTTTGGAC ACATGTCACT TAACACGTCC
    AATAAACCAA ACCCATCAGG GGTCCCCTGG GATGCTCACC CCCTGGAGGG GTCTCATTTT
    GATTTGGCAA GGACTAGTGA GAGTCCAACA GAACTGAAGA TAGACATTTC TCAGCCTCCT
    GATGTCAACT CTGAACTCTG GACAACGGGG CCGGGCCATC AGAGACCTGC TCTCCAAGAA
    AACAGCAAGG AGTCTTATAA AAATGTTGCA GATTTGGACT CCTTCCAGAG TGAATCGTCA
    TCTCCCCAGG ACTCCAATCA GCGTGGCCCC CAGCCACAGA CACTCACAAC AGAAGCAAAA
    CTAAGTCTTC CGATGGCGGC TGGACACGGT GGCGATGCTG GAGACGGGGC TGAAGAAAAC
    ATTCTTTACC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu78410
    Clone ID Related Accession (Same CDS sequence) XM_017315362.1 , XM_017315362.2
    Accession Version XM_017315362.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4155bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 28 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315362.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTTTTTGA CTTCTGGAGC CTGTCGGCAC TGTACAGCCG TGATGTCCCT GAGGTACCCT 
    GTGTTCCTGC ATGAAGATCG CTCCTTTGAT GGCCTCAAAA AATCCAAGCA TGTCCCAGCG
    TCCCTTGCTG CAGGCCAGCT CTCTGATGTT CTGAACGGGG GTGATGAAGT CTACGCTAAC
    TGTATGGTGA TTGACCAGGT TGGCGATTTG GATATCAACT ATATAAACCT AGAGGGACTC
    TCCACCCATA CGAGTCCCGA GTCTGGGAGG TCCATGCTGG GGCCTCAGGC TTGCATGCAC
    ACCCTTCCTC CCGACACCAG TCCCTGCGGG CGCCCACTCA TTGAGAACAG TGAGGGGACC
    CTGGATGCTG CAGCTTCGCA GTCTTTCGTG ACACCATCCA GTTCTCGCAC TTCCAACTTG
    AACCTTTCTT TTGGTCTCCA TGGATTTGAA AAGGAGCAGA GTCATCTCAA GAAAAGGAGT
    TCTAGCCTGG ATGCCCTGGT TGCCGACAGT GAGGGGGAAG GAGGTTCTGA GCCCCCCATC
    TGCTATGCTG TGGGCTCTCA GAGTTCCCCA AGAACTGGAC TCCCCAGCGG GGATGAACTG
    GACTCTTTTG AGACCAACAC TGAACCAGAC TGTAATATCT CCAGGACCGA ATCACTCTCT
    CTGTCCAGTA CTCTGCATTC AAAGGAGTCC TTGCTTTCTG GGATCCGGTC TCGCTCCTAC
    TCCTGCTCAT CACCCAAAAT CTCTTCAGGA AAATCCCGGC TAGTGCGCGA CTTCACAGTG
    TGCAGTACCA GCGAAGAACA GAGATCATAT AGTTTCCAGG AGCCACCAGG AGAAAAAAGG
    ATTCAGGAAG AAGAATGGGA TGAATACGTC ATTCCTGCCA AATCCGAGTC TGAAAAGTAC
    AAAGTGAGCC GAACTTTCAG CTTCCTCATG AACAGGATGA CCAGCCCACG GAACAAATCT
    AAGATGAAAA ACAAGGACAC TAAAGAAAAA GAAAAAATGA ATCGACATCA GTTTGTCCCG
    GGAACGTTCT CCGGGGTTCT GCAGTGCTCC GGTTGTGATA AAACACTCCT GGGGAAAGAG
    TCACTCCAGT GTGCCAACTG TAAGGCAAAC ACACACAAGG GCTGCAAAGA TGCTGTGCCT
    CCGTGTACCA AGAAATTCCA AGAGAAATAC AACAAGAACA AACCACAGAG CATCCTTGGA
    AGCTCTTCAG TCAGAGACGT CCCAGCACCT GGGCTCTCCT TGCACCCTTC TTCCTCTATG
    CCCATCGGAC TGCCAGCTGG AAGGAAGGAA TTTGCGGCTC AGGTGCATCC ACTGTCCAGA
    AGTGTCCCCG GCACCACTTT GGAAAGCTTC AGGAGGGCAG TGACATCGTT AGAGTCTGAG
    GGTGACAGCT GGAGAAGCAG GTCTCATTCC GATGAGCTGT TCCAGTCTAT GGGCTCTTCT
    CCTTCCACGG AGTCATTCAT GATGGAAGAT GTTGTGGATT CTTCTCTGTG GATCGACCTC
    AGTAGTGATG CTCAGGAGTT TGAAGCAGAG TCTTGGAGTC TCGTGGTGGA CCCTTCCTTT
    TGCAGTAGGC AGGAGAAGGA TGTCATCAAA AGACAAGATG TCATTTTTGA GCTGATGCAG
    ACAGAGGTCC ACCACATCCA GACTCTGCTC ATCATGTCCG AGGTCTTCAG GAAAGGCATG
    AAGGAGGAAC TGCAGCTGGA CCACAGCACG GTGGACAAGA TCTTCCCCTG CTTAGATGAG
    CTGCTGGAGA CTCACCGGCA CTTCTTCTTC AGCATGAAGG AGCGCAGGCA GGAGTCCTGC
    GCTGGCAGTG ACAGGAACTT TGTGATCAAC CAAATTGGAG ACATTCTGGT ACAACAGTTT
    TCAGAGGAAA ATGCAAGCAA AATGAAGAGG ATCTATGGAG AATTCTGCAG CCATCACAAA
    GAAGCCATGA GTCTCTTTAA AGAGCTGCAG CAGAACAAGA AGTTTCAGAA TTTTATTAAG
    ATCCGAAATA GCAACCTGTT GGCTCGTCGC CGCGGAATCC CGGAGTGCAT CCTGCTAGTT
    ACCCAGCGCA TCACAAAGTA CCCAGTCTTG GTGGAAAGGA TCCTGCAGTA CACCAAGGAA
    AGGACTGAAG AGCACCGGGA CCTGTGCAAA GCTCTCGGCT TAATTAAAGA CATGATCGCA
    GCCGTGGACC TGAAAGTCAG CGAATACGAG AAGAACCAAA AATGGCTCGA GATCCTAAAT
    AAAATCGAGA ACAAAACATA CACAAAGCTC AAAAATGGCC ACGTGTTTAG AAAGCAGGCG
    CTGCTGAGCC AGGAACGGGC TCTCCTGCAT GATGGGCTTG TCTACTGGAA AACCGCTACG
    GGTCGCTTCA AAGACATCCT GGCTCTGCTA CTAACTGACG TGCTGCTCTT CTTACAAGAA
    AAAGACCAGA AATACATCTT TGCAGCTGTT GACCAGAAGC CATCAGTGAT TTCCCTCCAG
    AAGCTGATTG CTAGAGAAGT TGCCAACGAG GAGAGAGGCA TGTTTCTGAT CAGTGCTTCG
    TCAGCCGGCC CCGAGATGTA CGAAATCCAC ACCAATTCCA AGGAGGAGCG GAATAACTGG
    ATGAGGCGGA TCCAGCAGGC AGTAGAAAGT TGTCCAGAGG AAGAAGGAGG AAGGACGAGC
    GAGTCGGATG AAGAGAGGCG GAAAGCTGAA GCTAGAGTGG CCAAGATTCA GCAATGTCAA
    GAAATACTCA GTAACCAAGA CCAACAGATT TGCACGTACT TGGAAGAAAA GCTGCATATC
    TACGCTGAAC TTGGGGAACT GAGTGGCTTT GAGGATGTCC ACCTAGAACC CCATCTGCTC
    ATCAAACCTG ACCCTGGAGA GCCTCCCCAG GCAGCGTCCC TGCTTGCAGC AGCCCTGAGA
    GAAGCCGAGA GTCTGCAAGT GGCTGTGAAG GCTTCAAAGA TGGGCGATGT AAGCCAGTCA
    TCCGAAGAAA GCCCTGGAGG GACTGTCCTG ATGGACACAC CCAGTACACA GGATGTACCA
    GCGTCACCAA CTGCTTCATT AGTCACAGAA GGGACAGAAG GAAGAGGCTG TTGGGATGTG
    GATCCCGGGC TCCAGGGTGT GGTAACCGAC TTGGCAGTCT CTGATGCAGG GGAGAAGGTG
    GAATATAGAA GTTTCTCCGG TTCTTCACAG TCAGAGATTA TACAAGCAAT ACAGAATTTA
    ACGCGTCTCC TCTACAGCCT TCAGGCTGCC TTGACCATCC AGGACAGTCA CATTGAGATC
    CACAAACTGG TTCTACAGCA GCGGGAGAGC TTGGCCCCTA GCCACTCCTT CCGAGGTGGC
    CCCTTGCAAG ACCAGGAGAA ATCCCGCTAC CTGGAAAAGC AGCGTGAAGA GCTGGCCAAC
    ATCCACAAGC TTCAACACCA GTTCCAGCAG GAGCAGCGGC GCTGGCACCG CACGTGCGAC
    CAGCAACAGC GCGAGCAGGA AGCCCAAGAG AGCTGGCTGC AGGCGCGGGA ACGTGAGTGC
    CAGTCCCAGG AGGAATTGTT GCTGCGCCAC CGTAGCGAGC TGGACCACCA ACTCCAGGAG
    TACCAGCAGA GCCTTGAGCG GCTACGGGAG GGCCAGCGGA TGGTGGAGAG GGAGAGACAG
    AAGATGCGTG TCCAGCAGGG TCTGCTGGGC CACTGCAAGC ATAGTCGCCA GCGGAGCCTC
    CCAGCCGTCT TCTCTCCAGG GAGCAAGGAG GTAACAGAAC TTAACCGAGC TGAGAGTTTG
    TGTCATGAAA ATTCATTTTT CATCAATGAA GCTTTTGGAC ACATGTCACT TAACACGTCC
    AATAAACCAA ACCCATCAGG GGTCCCCTGG GATGCTCACC CCCTGGAGGG GTCTCATTTT
    GATTTGGCAA GGACTAGTGA GAGTCCAACA GAACTGAAGA TAGACATTTC TCAGCCTCCT
    GATGTCAACT CTGAACTCTG GACAACGGGG CCGGGCCATC AGAGACCTGC TCTCCAAGAA
    AACAGCAAGG AGTCTTATAA AAATGTTGCA GATTTGGACT CCTTCCAGAG TGAATCGTCA
    TCTCCCCAGG ACTCCAATCA GCGTGGCCCC CAGCCACAGA CACTCACAAC AGAAGCAAAA
    CTAAGTCTTC CGATGGCGGC TGGACACGGT GGCGATGCTG GAGACGGGGC TGAAGAAAAC
    ATTCTTTACC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170851.1
    CDS219..4373
    Translation

    Target ORF information:

    RefSeq Version XM_017315362.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017315362.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTTTTTGA CTTCTGGAGC CTGTCGGCAC TGTACAGCCG TGATGTCCCT GAGGTACCCT 
    GTGTTCCTGC ATGAAGATCG CTCCTTTGAT GGCCTCAAAA AATCCAAGCA TGTCCCAGCG
    TCCCTTGCTG CAGGCCAGCT CTCTGATGTT CTGAACGGGG GTGATGAAGT CTACGCTAAC
    TGTATGGTGA TTGACCAGGT TGGCGATTTG GATATCAACT ATATAAACCT AGAGGGACTC
    TCCACCCATA CGAGTCCCGA GTCTGGGAGG TCCATGCTGG GGCCTCAGGC TTGCATGCAC
    ACCCTTCCTC CCGACACCAG TCCCTGCGGG CGCCCACTCA TTGAGAACAG TGAGGGGACC
    CTGGATGCTG CAGCTTCGCA GTCTTTCGTG ACACCATCCA GTTCTCGCAC TTCCAACTTG
    AACCTTTCTT TTGGTCTCCA TGGATTTGAA AAGGAGCAGA GTCATCTCAA GAAAAGGAGT
    TCTAGCCTGG ATGCCCTGGT TGCCGACAGT GAGGGGGAAG GAGGTTCTGA GCCCCCCATC
    TGCTATGCTG TGGGCTCTCA GAGTTCCCCA AGAACTGGAC TCCCCAGCGG GGATGAACTG
    GACTCTTTTG AGACCAACAC TGAACCAGAC TGTAATATCT CCAGGACCGA ATCACTCTCT
    CTGTCCAGTA CTCTGCATTC AAAGGAGTCC TTGCTTTCTG GGATCCGGTC TCGCTCCTAC
    TCCTGCTCAT CACCCAAAAT CTCTTCAGGA AAATCCCGGC TAGTGCGCGA CTTCACAGTG
    TGCAGTACCA GCGAAGAACA GAGATCATAT AGTTTCCAGG AGCCACCAGG AGAAAAAAGG
    ATTCAGGAAG AAGAATGGGA TGAATACGTC ATTCCTGCCA AATCCGAGTC TGAAAAGTAC
    AAAGTGAGCC GAACTTTCAG CTTCCTCATG AACAGGATGA CCAGCCCACG GAACAAATCT
    AAGATGAAAA ACAAGGACAC TAAAGAAAAA GAAAAAATGA ATCGACATCA GTTTGTCCCG
    GGAACGTTCT CCGGGGTTCT GCAGTGCTCC GGTTGTGATA AAACACTCCT GGGGAAAGAG
    TCACTCCAGT GTGCCAACTG TAAGGCAAAC ACACACAAGG GCTGCAAAGA TGCTGTGCCT
    CCGTGTACCA AGAAATTCCA AGAGAAATAC AACAAGAACA AACCACAGAG CATCCTTGGA
    AGCTCTTCAG TCAGAGACGT CCCAGCACCT GGGCTCTCCT TGCACCCTTC TTCCTCTATG
    CCCATCGGAC TGCCAGCTGG AAGGAAGGAA TTTGCGGCTC AGGTGCATCC ACTGTCCAGA
    AGTGTCCCCG GCACCACTTT GGAAAGCTTC AGGAGGGCAG TGACATCGTT AGAGTCTGAG
    GGTGACAGCT GGAGAAGCAG GTCTCATTCC GATGAGCTGT TCCAGTCTAT GGGCTCTTCT
    CCTTCCACGG AGTCATTCAT GATGGAAGAT GTTGTGGATT CTTCTCTGTG GATCGACCTC
    AGTAGTGATG CTCAGGAGTT TGAAGCAGAG TCTTGGAGTC TCGTGGTGGA CCCTTCCTTT
    TGCAGTAGGC AGGAGAAGGA TGTCATCAAA AGACAAGATG TCATTTTTGA GCTGATGCAG
    ACAGAGGTCC ACCACATCCA GACTCTGCTC ATCATGTCCG AGGTCTTCAG GAAAGGCATG
    AAGGAGGAAC TGCAGCTGGA CCACAGCACG GTGGACAAGA TCTTCCCCTG CTTAGATGAG
    CTGCTGGAGA CTCACCGGCA CTTCTTCTTC AGCATGAAGG AGCGCAGGCA GGAGTCCTGC
    GCTGGCAGTG ACAGGAACTT TGTGATCAAC CAAATTGGAG ACATTCTGGT ACAACAGTTT
    TCAGAGGAAA ATGCAAGCAA AATGAAGAGG ATCTATGGAG AATTCTGCAG CCATCACAAA
    GAAGCCATGA GTCTCTTTAA AGAGCTGCAG CAGAACAAGA AGTTTCAGAA TTTTATTAAG
    ATCCGAAATA GCAACCTGTT GGCTCGTCGC CGCGGAATCC CGGAGTGCAT CCTGCTAGTT
    ACCCAGCGCA TCACAAAGTA CCCAGTCTTG GTGGAAAGGA TCCTGCAGTA CACCAAGGAA
    AGGACTGAAG AGCACCGGGA CCTGTGCAAA GCTCTCGGCT TAATTAAAGA CATGATCGCA
    GCCGTGGACC TGAAAGTCAG CGAATACGAG AAGAACCAAA AATGGCTCGA GATCCTAAAT
    AAAATCGAGA ACAAAACATA CACAAAGCTC AAAAATGGCC ACGTGTTTAG AAAGCAGGCG
    CTGCTGAGCC AGGAACGGGC TCTCCTGCAT GATGGGCTTG TCTACTGGAA AACCGCTACG
    GGTCGCTTCA AAGACATCCT GGCTCTGCTA CTAACTGACG TGCTGCTCTT CTTACAAGAA
    AAAGACCAGA AATACATCTT TGCAGCTGTT GACCAGAAGC CATCAGTGAT TTCCCTCCAG
    AAGCTGATTG CTAGAGAAGT TGCCAACGAG GAGAGAGGCA TGTTTCTGAT CAGTGCTTCG
    TCAGCCGGCC CCGAGATGTA CGAAATCCAC ACCAATTCCA AGGAGGAGCG GAATAACTGG
    ATGAGGCGGA TCCAGCAGGC AGTAGAAAGT TGTCCAGAGG AAGAAGGAGG AAGGACGAGC
    GAGTCGGATG AAGAGAGGCG GAAAGCTGAA GCTAGAGTGG CCAAGATTCA GCAATGTCAA
    GAAATACTCA GTAACCAAGA CCAACAGATT TGCACGTACT TGGAAGAAAA GCTGCATATC
    TACGCTGAAC TTGGGGAACT GAGTGGCTTT GAGGATGTCC ACCTAGAACC CCATCTGCTC
    ATCAAACCTG ACCCTGGAGA GCCTCCCCAG GCAGCGTCCC TGCTTGCAGC AGCCCTGAGA
    GAAGCCGAGA GTCTGCAAGT GGCTGTGAAG GCTTCAAAGA TGGGCGATGT AAGCCAGTCA
    TCCGAAGAAA GCCCTGGAGG GACTGTCCTG ATGGACACAC CCAGTACACA GGATGTACCA
    GCGTCACCAA CTGCTTCATT AGTCACAGAA GGGACAGAAG GAAGAGGCTG TTGGGATGTG
    GATCCCGGGC TCCAGGGTGT GGTAACCGAC TTGGCAGTCT CTGATGCAGG GGAGAAGGTG
    GAATATAGAA GTTTCTCCGG TTCTTCACAG TCAGAGATTA TACAAGCAAT ACAGAATTTA
    ACGCGTCTCC TCTACAGCCT TCAGGCTGCC TTGACCATCC AGGACAGTCA CATTGAGATC
    CACAAACTGG TTCTACAGCA GCGGGAGAGC TTGGCCCCTA GCCACTCCTT CCGAGGTGGC
    CCCTTGCAAG ACCAGGAGAA ATCCCGCTAC CTGGAAAAGC AGCGTGAAGA GCTGGCCAAC
    ATCCACAAGC TTCAACACCA GTTCCAGCAG GAGCAGCGGC GCTGGCACCG CACGTGCGAC
    CAGCAACAGC GCGAGCAGGA AGCCCAAGAG AGCTGGCTGC AGGCGCGGGA ACGTGAGTGC
    CAGTCCCAGG AGGAATTGTT GCTGCGCCAC CGTAGCGAGC TGGACCACCA ACTCCAGGAG
    TACCAGCAGA GCCTTGAGCG GCTACGGGAG GGCCAGCGGA TGGTGGAGAG GGAGAGACAG
    AAGATGCGTG TCCAGCAGGG TCTGCTGGGC CACTGCAAGC ATAGTCGCCA GCGGAGCCTC
    CCAGCCGTCT TCTCTCCAGG GAGCAAGGAG GTAACAGAAC TTAACCGAGC TGAGAGTTTG
    TGTCATGAAA ATTCATTTTT CATCAATGAA GCTTTTGGAC ACATGTCACT TAACACGTCC
    AATAAACCAA ACCCATCAGG GGTCCCCTGG GATGCTCACC CCCTGGAGGG GTCTCATTTT
    GATTTGGCAA GGACTAGTGA GAGTCCAACA GAACTGAAGA TAGACATTTC TCAGCCTCCT
    GATGTCAACT CTGAACTCTG GACAACGGGG CCGGGCCATC AGAGACCTGC TCTCCAAGAA
    AACAGCAAGG AGTCTTATAA AAATGTTGCA GATTTGGACT CCTTCCAGAG TGAATCGTCA
    TCTCCCCAGG ACTCCAATCA GCGTGGCCCC CAGCCACAGA CACTCACAAC AGAAGCAAAA
    CTAAGTCTTC CGATGGCGGC TGGACACGGT GGCGATGCTG GAGACGGGGC TGAAGAAAAC
    ATTCTTTACC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu86213
    Clone ID Related Accession (Same CDS sequence) XM_030247128.1
    Accession Version XM_030247128.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4227bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 28 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCCTTCTG GAGCTGCAGA AACTGAAGAA GAGGTTAGGA ATTTGGAGTC TGGCAGATCA 
    CCATCGGAGG AGGAGGAGGA TGCAAAGAGC ATCAAAAGCC AGGTGGATGG GCCGAGTGAA
    CACGAAGACC AGGACCGCCT ACCTCTGGAT CGCTCCTTTG ATGGCCTCAA AAAATCCAAG
    CATGTCCCAG CGTCCCTTGC TGCAGGCCAG CTCTCTGATG TTCTGAACGG GGGTGATGAA
    GTCTACGCTA ACTGTATGGT GATTGACCAG GTTGGCGATT TGGATATCAA CTATATAAAC
    CTAGAGGGAC TCTCCACCCA TACGAGTCCC GAGTCTGGGA GGTCCATGCT GGGGCCTCAG
    GCTTGCATGC ACACCCTTCC TCCCGACACC AGTCCCTGCG GGCGCCCACT CATTGAGAAC
    AGTGAGGGGA CCCTGGATGC TGCAGCTTCG CAGTCTTTCG TGACACCATC CAGTTCTCGC
    ACTTCCAACT TGAACCTTTC TTTTGGTCTC CATGGATTTG AAAAGGAGCA GAGTCATCTC
    AAGAAAAGGA GTTCTAGCCT GGATGCCCTG GTTGCCGACA GTGAGGGGGA AGGAGGTTCT
    GAGCCCCCCA TCTGCTATGC TGTGGGCTCT CAGAGTTCCC CAAGAACTGG ACTCCCCAGC
    GGGGATGAAC TGGACTCTTT TGAGACCAAC ACTGAACCAG ACTGTAATAT CTCCAGGACC
    GAATCACTCT CTCTGTCCAG TACTCTGCAT TCAAAGGAGT CCTTGCTTTC TGGGATCCGG
    TCTCGCTCCT ACTCCTGCTC ATCACCCAAA ATCTCTTCAG GAAAATCCCG GCTAGTGCGC
    GACTTCACAG TGTGCAGTAC CAGCGAAGAA CAGAGATCAT ATAGTTTCCA GGAGCCACCA
    GGAGAAAAAA GGATTCAGGA AGAAGAATGG GATGAATACG TCATTCCTGC CAAATCCGAG
    TCTGAAAAGT ACAAAGTGAG CCGAACTTTC AGCTTCCTCA TGAACAGGAT GACCAGCCCA
    CGGAACAAAT CTAAGATGAA AAACAAGGAC ACTAAAGAAA AAGAAAAAAT GAATCGACAT
    CAGTTTGTCC CGGGAACGTT CTCCGGGGTT CTGCAGTGCT CCGGTTGTGA TAAAACACTC
    CTGGGGAAAG AGTCACTCCA GTGTGCCAAC TGTAAGGCAA ACACACACAA GGGCTGCAAA
    GATGCTGTGC CTCCGTGTAC CAAGAAATTC CAAGAGAAAT ACAACAAGAA CAAACCACAG
    AGCATCCTTG GAAGCTCTTC AGTCAGAGAC GTCCCAGCAC CTGGGCTCTC CTTGCACCCT
    TCTTCCTCTA TGCCCATCGG ACTGCCAGCT GGAAGGAAGG AATTTGCGGC TCAGGTGCAT
    CCACTGTCCA GAAGTGTCCC CGGCACCACT TTGGAAAGCT TCAGGAGGGC AGTGACATCG
    TTAGAGTCTG AGGGTGACAG CTGGAGAAGC AGGTCTCATT CCGATGAGCT GTTCCAGTCT
    ATGGGCTCTT CTCCTTCCAC GGAGTCATTC ATGATGGAAG ATGTTGTGGA TTCTTCTCTG
    TGGATCGACC TCAGTAGTGA TGCTCAGGAG TTTGAAGCAG AGTCTTGGAG TCTCGTGGTG
    GACCCTTCCT TTTGCAGTAG GCAGGAGAAG GATGTCATCA AAAGACAAGA TGTCATTTTT
    GAGCTGATGC AGACAGAGGT CCACCACATC CAGACTCTGC TCATCATGTC CGAGGTCTTC
    AGGAAAGGCA TGAAGGAGGA ACTGCAGCTG GACCACAGCA CGGTGGACAA GATCTTCCCC
    TGCTTAGATG AGCTGCTGGA GACTCACCGG CACTTCTTCT TCAGCATGAA GGAGCGCAGG
    CAGGAGTCCT GCGCTGGCAG TGACAGGAAC TTTGTGATCA ACCAAATTGG AGACATTCTG
    GTACAACAGT TTTCAGAGGA AAATGCAAGC AAAATGAAGA GGATCTATGG AGAATTCTGC
    AGCCATCACA AAGAAGCCAT GAGTCTCTTT AAAGAGCTGC AGCAGAACAA GAAGTTTCAG
    AATTTTATTA AGATCCGAAA TAGCAACCTG TTGGCTCGTC GCCGCGGAAT CCCGGAGTGC
    ATCCTGCTAG TTACCCAGCG CATCACAAAG TACCCAGTCT TGGTGGAAAG GATCCTGCAG
    TACACCAAGG AAAGGACTGA AGAGCACCGG GACCTGTGCA AAGCTCTCGG CTTAATTAAA
    GACATGATCG CAGCCGTGGA CCTGAAAGTC AGCGAATACG AGAAGAACCA AAAATGGCTC
    GAGATCCTAA ATAAAATCGA GAACAAAACA TACACAAAGC TCAAAAATGG CCACGTGTTT
    AGAAAGCAGG CGCTGCTGAG CCAGGAACGG GCTCTCCTGC ATGATGGGCT TGTCTACTGG
    AAAACCGCTA CGGGTCGCTT CAAAGACATC CTGGCTCTGC TACTAACTGA CGTGCTGCTC
    TTCTTACAAG AAAAAGACCA GAAATACATC TTTGCAGCTG TTGACCAGAA GCCATCAGTG
    ATTTCCCTCC AGAAGCTGAT TGCTAGAGAA GTTGCCAACG AGGAGAGAGG CATGTTTCTG
    ATCAGTGCTT CGTCAGCCGG CCCCGAGATG TACGAAATCC ACACCAATTC CAAGGAGGAG
    CGGAATAACT GGATGAGGCG GATCCAGCAG GCAGTAGAAA GTTGTCCAGA GGAAGAAGGA
    GGAAGGACGA GCGAGTCGGA TGAAGAGAGG CGGAAAGCTG AAGCTAGAGT GGCCAAGATT
    CAGCAATGTC AAGAAATACT CAGTAACCAA GACCAACAGA TTTGCACGTA CTTGGAAGAA
    AAGCTGCATA TCTACGCTGA ACTTGGGGAA CTGAGTGGCT TTGAGGATGT CCACCTAGAA
    CCCCATCTGC TCATCAAACC TGACCCTGGA GAGCCTCCCC AGGCAGCGTC CCTGCTTGCA
    GCAGCCCTGA GAGAAGCCGA GAGTCTGCAA GTGGCTGTGA AGGCTTCAAA GATGGGCGAT
    GTAAGCCAGT CATCCGAAGA AAGCCCTGGA GGGACTGTCC TGATGGACAC ACCCAGTACA
    CAGGATGTAC CAGCGTCACC AACTGCTTCA TTAGTCACAG AAGGGACAGA AGGAAGAGGC
    TGTTGGGATG TGGATCCCGG GCTCCAGGGT GTGGTAACCG ACTTGGCAGT CTCTGATGCA
    GGGGAGAAGG TGGAATATAG AAGTTTCTCC GGTTCTTCAC AGTCAGAGAT TATACAAGCA
    ATACAGAATT TAACGCGTCT CCTCTACAGC CTTCAGGCTG CCTTGACCAT CCAGGACAGT
    CACATTGAGA TCCACAAACT GGTTCTACAG CAGCGGGAGA GCTTGGCCCC TAGCCACTCC
    TTCCGAGGTG GCCCCTTGCA AGACCAGGAG AAATCCCGCT ACCTGGAAAA GCAGCGTGAA
    GAGCTGGCCA ACATCCACAA GCTTCAACAC CAGTTCCAGC AGGAGCAGCG GCGCTGGCAC
    CGCACGTGCG ACCAGCAACA GCGCGAGCAG GAAGCCCAAG AGAGCTGGCT GCAGGCGCGG
    GAACGTGAGT GCCAGTCCCA GGAGGAATTG TTGCTGCGCC ACCGTAGCGA GCTGGACCAC
    CAACTCCAGG AGTACCAGCA GAGCCTTGAG CGGCTACGGG AGGGCCAGCG GATGGTGGAG
    AGGGAGAGAC AGAAGATGCG TGTCCAGCAG GGTCTGCTGG GCCACTGCAA GCATAGTCGC
    CAGCGGAGCC TCCCAGCCGT CTTCTCTCCA GGGAGCAAGG AGGTAACAGA ACTTAACCGA
    GCTGAGAGTT TGTGTCATGA AAATTCATTT TTCATCAATG AAGCTTTTGG ACACATGTCA
    CTTAACACGT CCAATAAACC AAACCCATCA GGGGTCCCCT GGGATGCTCA CCCCCTGGAG
    GGGTCTCATT TTGATTTGGC AAGGACTAGT GAGAGTCCAA CAGAACTGAA GATAGACATT
    TCTCAGCCTC CTGATGTCAA CTCTGAACTC TGGACAACGG GGCCGGGCCA TCAGAGACCT
    GCTCTCCAAG AAAACAGCAA GGAGTCTTAT AAAAATGTTG CAGATTTGGA CTCCTTCCAG
    AGTGAATCGT CATCTCCCCA GGACTCCAAT CAGCGTGGCC CCCAGCCACA GACACTCACA
    ACAGAAGCAA AACTAAGTCT TCCGATGGCG GCTGGACACG GTGGCGATGC TGGAGACGGG
    GCTGAAGAAA ACATTCTTTA CCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030102988.1
    CDS352..4578
    Translation

    Target ORF information:

    RefSeq Version XM_030247128.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030247128.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCCTTCTG GAGCTGCAGA AACTGAAGAA GAGGTTAGGA ATTTGGAGTC TGGCAGATCA 
    CCATCGGAGG AGGAGGAGGA TGCAAAGAGC ATCAAAAGCC AGGTGGATGG GCCGAGTGAA
    CACGAAGACC AGGACCGCCT ACCTCTGGAT CGCTCCTTTG ATGGCCTCAA AAAATCCAAG
    CATGTCCCAG CGTCCCTTGC TGCAGGCCAG CTCTCTGATG TTCTGAACGG GGGTGATGAA
    GTCTACGCTA ACTGTATGGT GATTGACCAG GTTGGCGATT TGGATATCAA CTATATAAAC
    CTAGAGGGAC TCTCCACCCA TACGAGTCCC GAGTCTGGGA GGTCCATGCT GGGGCCTCAG
    GCTTGCATGC ACACCCTTCC TCCCGACACC AGTCCCTGCG GGCGCCCACT CATTGAGAAC
    AGTGAGGGGA CCCTGGATGC TGCAGCTTCG CAGTCTTTCG TGACACCATC CAGTTCTCGC
    ACTTCCAACT TGAACCTTTC TTTTGGTCTC CATGGATTTG AAAAGGAGCA GAGTCATCTC
    AAGAAAAGGA GTTCTAGCCT GGATGCCCTG GTTGCCGACA GTGAGGGGGA AGGAGGTTCT
    GAGCCCCCCA TCTGCTATGC TGTGGGCTCT CAGAGTTCCC CAAGAACTGG ACTCCCCAGC
    GGGGATGAAC TGGACTCTTT TGAGACCAAC ACTGAACCAG ACTGTAATAT CTCCAGGACC
    GAATCACTCT CTCTGTCCAG TACTCTGCAT TCAAAGGAGT CCTTGCTTTC TGGGATCCGG
    TCTCGCTCCT ACTCCTGCTC ATCACCCAAA ATCTCTTCAG GAAAATCCCG GCTAGTGCGC
    GACTTCACAG TGTGCAGTAC CAGCGAAGAA CAGAGATCAT ATAGTTTCCA GGAGCCACCA
    GGAGAAAAAA GGATTCAGGA AGAAGAATGG GATGAATACG TCATTCCTGC CAAATCCGAG
    TCTGAAAAGT ACAAAGTGAG CCGAACTTTC AGCTTCCTCA TGAACAGGAT GACCAGCCCA
    CGGAACAAAT CTAAGATGAA AAACAAGGAC ACTAAAGAAA AAGAAAAAAT GAATCGACAT
    CAGTTTGTCC CGGGAACGTT CTCCGGGGTT CTGCAGTGCT CCGGTTGTGA TAAAACACTC
    CTGGGGAAAG AGTCACTCCA GTGTGCCAAC TGTAAGGCAA ACACACACAA GGGCTGCAAA
    GATGCTGTGC CTCCGTGTAC CAAGAAATTC CAAGAGAAAT ACAACAAGAA CAAACCACAG
    AGCATCCTTG GAAGCTCTTC AGTCAGAGAC GTCCCAGCAC CTGGGCTCTC CTTGCACCCT
    TCTTCCTCTA TGCCCATCGG ACTGCCAGCT GGAAGGAAGG AATTTGCGGC TCAGGTGCAT
    CCACTGTCCA GAAGTGTCCC CGGCACCACT TTGGAAAGCT TCAGGAGGGC AGTGACATCG
    TTAGAGTCTG AGGGTGACAG CTGGAGAAGC AGGTCTCATT CCGATGAGCT GTTCCAGTCT
    ATGGGCTCTT CTCCTTCCAC GGAGTCATTC ATGATGGAAG ATGTTGTGGA TTCTTCTCTG
    TGGATCGACC TCAGTAGTGA TGCTCAGGAG TTTGAAGCAG AGTCTTGGAG TCTCGTGGTG
    GACCCTTCCT TTTGCAGTAG GCAGGAGAAG GATGTCATCA AAAGACAAGA TGTCATTTTT
    GAGCTGATGC AGACAGAGGT CCACCACATC CAGACTCTGC TCATCATGTC CGAGGTCTTC
    AGGAAAGGCA TGAAGGAGGA ACTGCAGCTG GACCACAGCA CGGTGGACAA GATCTTCCCC
    TGCTTAGATG AGCTGCTGGA GACTCACCGG CACTTCTTCT TCAGCATGAA GGAGCGCAGG
    CAGGAGTCCT GCGCTGGCAG TGACAGGAAC TTTGTGATCA ACCAAATTGG AGACATTCTG
    GTACAACAGT TTTCAGAGGA AAATGCAAGC AAAATGAAGA GGATCTATGG AGAATTCTGC
    AGCCATCACA AAGAAGCCAT GAGTCTCTTT AAAGAGCTGC AGCAGAACAA GAAGTTTCAG
    AATTTTATTA AGATCCGAAA TAGCAACCTG TTGGCTCGTC GCCGCGGAAT CCCGGAGTGC
    ATCCTGCTAG TTACCCAGCG CATCACAAAG TACCCAGTCT TGGTGGAAAG GATCCTGCAG
    TACACCAAGG AAAGGACTGA AGAGCACCGG GACCTGTGCA AAGCTCTCGG CTTAATTAAA
    GACATGATCG CAGCCGTGGA CCTGAAAGTC AGCGAATACG AGAAGAACCA AAAATGGCTC
    GAGATCCTAA ATAAAATCGA GAACAAAACA TACACAAAGC TCAAAAATGG CCACGTGTTT
    AGAAAGCAGG CGCTGCTGAG CCAGGAACGG GCTCTCCTGC ATGATGGGCT TGTCTACTGG
    AAAACCGCTA CGGGTCGCTT CAAAGACATC CTGGCTCTGC TACTAACTGA CGTGCTGCTC
    TTCTTACAAG AAAAAGACCA GAAATACATC TTTGCAGCTG TTGACCAGAA GCCATCAGTG
    ATTTCCCTCC AGAAGCTGAT TGCTAGAGAA GTTGCCAACG AGGAGAGAGG CATGTTTCTG
    ATCAGTGCTT CGTCAGCCGG CCCCGAGATG TACGAAATCC ACACCAATTC CAAGGAGGAG
    CGGAATAACT GGATGAGGCG GATCCAGCAG GCAGTAGAAA GTTGTCCAGA GGAAGAAGGA
    GGAAGGACGA GCGAGTCGGA TGAAGAGAGG CGGAAAGCTG AAGCTAGAGT GGCCAAGATT
    CAGCAATGTC AAGAAATACT CAGTAACCAA GACCAACAGA TTTGCACGTA CTTGGAAGAA
    AAGCTGCATA TCTACGCTGA ACTTGGGGAA CTGAGTGGCT TTGAGGATGT CCACCTAGAA
    CCCCATCTGC TCATCAAACC TGACCCTGGA GAGCCTCCCC AGGCAGCGTC CCTGCTTGCA
    GCAGCCCTGA GAGAAGCCGA GAGTCTGCAA GTGGCTGTGA AGGCTTCAAA GATGGGCGAT
    GTAAGCCAGT CATCCGAAGA AAGCCCTGGA GGGACTGTCC TGATGGACAC ACCCAGTACA
    CAGGATGTAC CAGCGTCACC AACTGCTTCA TTAGTCACAG AAGGGACAGA AGGAAGAGGC
    TGTTGGGATG TGGATCCCGG GCTCCAGGGT GTGGTAACCG ACTTGGCAGT CTCTGATGCA
    GGGGAGAAGG TGGAATATAG AAGTTTCTCC GGTTCTTCAC AGTCAGAGAT TATACAAGCA
    ATACAGAATT TAACGCGTCT CCTCTACAGC CTTCAGGCTG CCTTGACCAT CCAGGACAGT
    CACATTGAGA TCCACAAACT GGTTCTACAG CAGCGGGAGA GCTTGGCCCC TAGCCACTCC
    TTCCGAGGTG GCCCCTTGCA AGACCAGGAG AAATCCCGCT ACCTGGAAAA GCAGCGTGAA
    GAGCTGGCCA ACATCCACAA GCTTCAACAC CAGTTCCAGC AGGAGCAGCG GCGCTGGCAC
    CGCACGTGCG ACCAGCAACA GCGCGAGCAG GAAGCCCAAG AGAGCTGGCT GCAGGCGCGG
    GAACGTGAGT GCCAGTCCCA GGAGGAATTG TTGCTGCGCC ACCGTAGCGA GCTGGACCAC
    CAACTCCAGG AGTACCAGCA GAGCCTTGAG CGGCTACGGG AGGGCCAGCG GATGGTGGAG
    AGGGAGAGAC AGAAGATGCG TGTCCAGCAG GGTCTGCTGG GCCACTGCAA GCATAGTCGC
    CAGCGGAGCC TCCCAGCCGT CTTCTCTCCA GGGAGCAAGG AGGTAACAGA ACTTAACCGA
    GCTGAGAGTT TGTGTCATGA AAATTCATTT TTCATCAATG AAGCTTTTGG ACACATGTCA
    CTTAACACGT CCAATAAACC AAACCCATCA GGGGTCCCCT GGGATGCTCA CCCCCTGGAG
    GGGTCTCATT TTGATTTGGC AAGGACTAGT GAGAGTCCAA CAGAACTGAA GATAGACATT
    TCTCAGCCTC CTGATGTCAA CTCTGAACTC TGGACAACGG GGCCGGGCCA TCAGAGACCT
    GCTCTCCAAG AAAACAGCAA GGAGTCTTAT AAAAATGTTG CAGATTTGGA CTCCTTCCAG
    AGTGAATCGT CATCTCCCCA GGACTCCAAT CAGCGTGGCC CCCAGCCACA GACACTCACA
    ACAGAAGCAA AACTAAGTCT TCCGATGGCG GCTGGACACG GTGGCGATGC TGGAGACGGG
    GCTGAAGAAA ACATTCTTTA CCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu48950
    Clone ID Related Accession (Same CDS sequence) XM_006517522.3
    Accession Version XM_006517522.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 28 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517522.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTGATTG ACCAGGTTGG CGATTTGGAT ATCAACTATA TAAACCTAGA GGGACTCTCC 
    ACCCATACGA GTCCCGAGTC TGGGAGGTCC ATGCTGGGGC CTCAGGCTTG CATGCACACC
    CTTCCTCCCG ACACCAGTCC CTGCGGGCGC CCACTCATTG AGAACAGTGA GGGGACCCTG
    GATGCTGCAG CTTCGCAGTC TTTCGTGACA CCATCCAGTT CTCGCACTTC CAACTTGAAC
    CTTTCTTTTG GTCTCCATGG ATTTGAAAAG GAGCAGAGTC ATCTCAAGAA AAGGAGTTCT
    AGCCTGGATG CCCTGGTTGC CGACAGTGAG GGGGAAGGAG GTTCTGAGCC CCCCATCTGC
    TATGCTGTGG GCTCTCAGAG TTCCCCAAGA ACTGGACTCC CCAGCGGGGA TGAACTGGAC
    TCTTTTGAGA CCAACACTGA ACCAGACTGT AATATCTCCA GGACCGAATC ACTCTCTCTG
    TCCAGTACTC TGCATTCAAA GGAGTCCTTG CTTTCTGGGA TCCGGTCTCG CTCCTACTCC
    TGCTCATCAC CCAAAATCTC TTCAGGAAAA TCCCGGCTAG TGCGCGACTT CACAGTGTGC
    AGTACCAGCG AAGAACAGAG ATCATATAGT TTCCAGGAGC CACCAGGAGA AAAAAGGATT
    CAGGAAGAAG AATGGGATGA ATACGTCATT CCTGCCAAAT CCGAGTCTGA AAAGTACAAA
    GTGAGCCGAA CTTTCAGCTT CCTCATGAAC AGGATGACCA GCCCACGGAA CAAATCTAAG
    ATGAAAAACA AGGACACTAA AGAAAAAGAA AAAATGAATC GACATCAGTT TGTCCCGGGA
    ACGTTCTCCG GGGTTCTGCA GTGCTCCGGT TGTGATAAAA CACTCCTGGG GAAAGAGTCA
    CTCCAGTGTG CCAACTGTAA GGCAAACACA CACAAGGGCT GCAAAGATGC TGTGCCTCCG
    TGTACCAAGA AATTCCAAGA GAAATACAAC AAGAACAAAC CACAGAGCAT CCTTGGAAGC
    TCTTCAGTCA GAGACGTCCC AGCACCTGGG CTCTCCTTGC ACCCTTCTTC CTCTATGCCC
    ATCGGACTGC CAGCTGGAAG GAAGGAATTT GCGGCTCAGG TGCATCCACT GTCCAGAAGT
    GTCCCCGGCA CCACTTTGGA AAGCTTCAGG AGGGCAGTGA CATCGTTAGA GTCTGAGGGT
    GACAGCTGGA GAAGCAGGTC TCATTCCGAT GAGCTGTTCC AGTCTATGGG CTCTTCTCCT
    TCCACGGAGT CATTCATGAT GGAAGATGTT GTGGATTCTT CTCTGTGGAT CGACCTCAGT
    AGTGATGCTC AGGAGTTTGA AGCAGAGTCT TGGAGTCTCG TGGTGGACCC TTCCTTTTGC
    AGTAGGCAGG AGAAGGATGT CATCAAAAGA CAAGATGTCA TTTTTGAGCT GATGCAGACA
    GAGGTCCACC ACATCCAGAC TCTGCTCATC ATGTCCGAGG TCTTCAGGAA AGGCATGAAG
    GAGGAACTGC AGCTGGACCA CAGCACGGTG GACAAGATCT TCCCCTGCTT AGATGAGCTG
    CTGGAGACTC ACCGGCACTT CTTCTTCAGC ATGAAGGAGC GCAGGCAGGA GTCCTGCGCT
    GGCAGTGACA GGAACTTTGT GATCAACCAA ATTGGAGACA TTCTGGTACA ACAGTTTTCA
    GAGGAAAATG CAAGCAAAAT GAAGAGGATC TATGGAGAAT TCTGCAGCCA TCACAAAGAA
    GCCATGAGTC TCTTTAAAGA GCTGCAGCAG AACAAGAAGT TTCAGAATTT TATTAAGATC
    CGAAATAGCA ACCTGTTGGC TCGTCGCCGC GGAATCCCGG AGTGCATCCT GCTAGTTACC
    CAGCGCATCA CAAAGTACCC AGTCTTGGTG GAAAGGATCC TGCAGTACAC CAAGGAAAGG
    ACTGAAGAGC ACCGGGACCT GTGCAAAGCT CTCGGCTTAA TTAAAGACAT GATCGCAGCC
    GTGGACCTGA AAGTCAGCGA ATACGAGAAG AACCAAAAAT GGCTCGAGAT CCTAAATAAA
    ATCGAGAACA AAACATACAC AAAGCTCAAA AATGGCCACG TGTTTAGAAA GCAGGCGCTG
    CTGAGCCAGG AACGGGCTCT CCTGCATGAT GGGCTTGTCT ACTGGAAAAC CGCTACGGGT
    CGCTTCAAAG ACATCCTGGC TCTGCTACTA ACTGACGTGC TGCTCTTCTT ACAAGAAAAA
    GACCAGAAAT ACATCTTTGC AGCTGTTGAC CAGAAGCCAT CAGTGATTTC CCTCCAGAAG
    CTGATTGCTA GAGAAGTTGC CAACGAGGAG AGAGGCATGT TTCTGATCAG TGCTTCGTCA
    GCCGGCCCCG AGATGTACGA AATCCACACC AATTCCAAGG AGGAGCGGAA TAACTGGATG
    AGGCGGATCC AGCAGGCAGT AGAAAGTTGT CCAGAGGAAG AAGGAGGAAG GACGAGCGAG
    TCGGATGAAG AGAGGCGGAA AGCTGAAGCT AGAGTGGCCA AGATTCAGCA ATGTCAAGAA
    ATACTCAGTA ACCAAGACCA ACAGATTTGC ACGTACTTGG AAGAAAAGCT GCATATCTAC
    GCTGAACTTG GGGAACTGAG TGGCTTTGAG GATGTCCACC TAGAACCCCA TCTGCTCATC
    AAACCTGACC CTGGAGAGCC TCCCCAGGCA GCGTCCCTGC TTGCAGCAGC CCTGAGAGAA
    GCCGAGAGTC TGCAAGTGGC TGTGAAGGCT TCAAAGATGG GCGATGTAAG CCAGTCATCC
    GAAGAAAGCC CTGGAGGGAC TGTCCTGATG GACACACCCA GTACACAGGA TGTACCAGCG
    TCACCAACTG CTTCATTAGT CACAGAAGGG ACAGAAGGAA GAGGCTGTTG GGATGTGGAT
    CCCGGGCTCC AGGGTGTGGT AACCGACTTG GCAGTCTCTG ATGCAGGGGA GAAGGTGGAA
    TATAGAAGTT TCTCCGGTTC TTCACAGTCA GAGATTATAC AAGCAATACA GAATTTAACG
    CGTCTCCTCT ACAGCCTTCA GGCTGCCTTG ACCATCCAGG ACAGTCACAT TGAGATCCAC
    AAACTGGTTC TACAGCAGCG GGAGAGCTTG GCCCCTAGCC ACTCCTTCCG AGGTGGCCCC
    TTGCAAGACC AGGAGAAATC CCGCTACCTG GAAAAGCAGC GTGAAGAGCT GGCCAACATC
    CACAAGCTTC AACACCAGTT CCAGCAGGAG CAGCGGCGCT GGCACCGCAC GTGCGACCAG
    CAACAGCGCG AGCAGGAAGC CCAAGAGAGC TGGCTGCAGG CGCGGGAACG TGAGTGCCAG
    TCCCAGGAGG AATTGTTGCT GCGCCACCGT AGCGAGCTGG ACCACCAACT CCAGGAGTAC
    CAGCAGAGCC TTGAGCGGCT ACGGGAGGGC CAGCGGATGG TGGAGAGGGA GAGACAGAAG
    ATGCGTGTCC AGCAGGGTCT GCTGGGCCAC TGCAAGCATA GTCGCCAGCG GAGCCTCCCA
    GCCGTCTTCT CTCCAGGGAG CAAGGAGGTA ACAGAACTTA ACCGAGCTGA GAGTTTGTGT
    CATGAAAATT CATTTTTCAT CAATGAAGCT TTTGGACACA TGTCACTTAA CACGTCCAAT
    AAACCAAACC CATCAGGGGT CCCCTGGGAT GCTCACCCCC TGGAGGGGTC TCATTTTGAT
    TTGGCAAGGA CTAGTGAGAG TCCAACAGAA CTGAAGATAG ACATTTCTCA GCCTCCTGAT
    GTCAACTCTG AACTCTGGAC AACGGGGCCG GGCCATCAGA GACCTGCTCT CCAAGAAAAC
    AGCAAGGAGT CTTATAAAAA TGTTGCAGAT TTGGACTCCT TCCAGAGTGA ATCGTCATCT
    CCCCAGGACT CCAATCAGCG TGGCCCCCAG CCACAGACAC TCACAACAGA AGCAAAACTA
    AGTCTTCCGA TGGCGGCTGG ACACGGTGGC GATGCTGGAG ACGGGGCTGA AGAAAACATT
    CTTTACCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006517585.1
    CDS322..4293
    Translation

    Target ORF information:

    RefSeq Version XM_006517522.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006517522.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTGATTG ACCAGGTTGG CGATTTGGAT ATCAACTATA TAAACCTAGA GGGACTCTCC 
    ACCCATACGA GTCCCGAGTC TGGGAGGTCC ATGCTGGGGC CTCAGGCTTG CATGCACACC
    CTTCCTCCCG ACACCAGTCC CTGCGGGCGC CCACTCATTG AGAACAGTGA GGGGACCCTG
    GATGCTGCAG CTTCGCAGTC TTTCGTGACA CCATCCAGTT CTCGCACTTC CAACTTGAAC
    CTTTCTTTTG GTCTCCATGG ATTTGAAAAG GAGCAGAGTC ATCTCAAGAA AAGGAGTTCT
    AGCCTGGATG CCCTGGTTGC CGACAGTGAG GGGGAAGGAG GTTCTGAGCC CCCCATCTGC
    TATGCTGTGG GCTCTCAGAG TTCCCCAAGA ACTGGACTCC CCAGCGGGGA TGAACTGGAC
    TCTTTTGAGA CCAACACTGA ACCAGACTGT AATATCTCCA GGACCGAATC ACTCTCTCTG
    TCCAGTACTC TGCATTCAAA GGAGTCCTTG CTTTCTGGGA TCCGGTCTCG CTCCTACTCC
    TGCTCATCAC CCAAAATCTC TTCAGGAAAA TCCCGGCTAG TGCGCGACTT CACAGTGTGC
    AGTACCAGCG AAGAACAGAG ATCATATAGT TTCCAGGAGC CACCAGGAGA AAAAAGGATT
    CAGGAAGAAG AATGGGATGA ATACGTCATT CCTGCCAAAT CCGAGTCTGA AAAGTACAAA
    GTGAGCCGAA CTTTCAGCTT CCTCATGAAC AGGATGACCA GCCCACGGAA CAAATCTAAG
    ATGAAAAACA AGGACACTAA AGAAAAAGAA AAAATGAATC GACATCAGTT TGTCCCGGGA
    ACGTTCTCCG GGGTTCTGCA GTGCTCCGGT TGTGATAAAA CACTCCTGGG GAAAGAGTCA
    CTCCAGTGTG CCAACTGTAA GGCAAACACA CACAAGGGCT GCAAAGATGC TGTGCCTCCG
    TGTACCAAGA AATTCCAAGA GAAATACAAC AAGAACAAAC CACAGAGCAT CCTTGGAAGC
    TCTTCAGTCA GAGACGTCCC AGCACCTGGG CTCTCCTTGC ACCCTTCTTC CTCTATGCCC
    ATCGGACTGC CAGCTGGAAG GAAGGAATTT GCGGCTCAGG TGCATCCACT GTCCAGAAGT
    GTCCCCGGCA CCACTTTGGA AAGCTTCAGG AGGGCAGTGA CATCGTTAGA GTCTGAGGGT
    GACAGCTGGA GAAGCAGGTC TCATTCCGAT GAGCTGTTCC AGTCTATGGG CTCTTCTCCT
    TCCACGGAGT CATTCATGAT GGAAGATGTT GTGGATTCTT CTCTGTGGAT CGACCTCAGT
    AGTGATGCTC AGGAGTTTGA AGCAGAGTCT TGGAGTCTCG TGGTGGACCC TTCCTTTTGC
    AGTAGGCAGG AGAAGGATGT CATCAAAAGA CAAGATGTCA TTTTTGAGCT GATGCAGACA
    GAGGTCCACC ACATCCAGAC TCTGCTCATC ATGTCCGAGG TCTTCAGGAA AGGCATGAAG
    GAGGAACTGC AGCTGGACCA CAGCACGGTG GACAAGATCT TCCCCTGCTT AGATGAGCTG
    CTGGAGACTC ACCGGCACTT CTTCTTCAGC ATGAAGGAGC GCAGGCAGGA GTCCTGCGCT
    GGCAGTGACA GGAACTTTGT GATCAACCAA ATTGGAGACA TTCTGGTACA ACAGTTTTCA
    GAGGAAAATG CAAGCAAAAT GAAGAGGATC TATGGAGAAT TCTGCAGCCA TCACAAAGAA
    GCCATGAGTC TCTTTAAAGA GCTGCAGCAG AACAAGAAGT TTCAGAATTT TATTAAGATC
    CGAAATAGCA ACCTGTTGGC TCGTCGCCGC GGAATCCCGG AGTGCATCCT GCTAGTTACC
    CAGCGCATCA CAAAGTACCC AGTCTTGGTG GAAAGGATCC TGCAGTACAC CAAGGAAAGG
    ACTGAAGAGC ACCGGGACCT GTGCAAAGCT CTCGGCTTAA TTAAAGACAT GATCGCAGCC
    GTGGACCTGA AAGTCAGCGA ATACGAGAAG AACCAAAAAT GGCTCGAGAT CCTAAATAAA
    ATCGAGAACA AAACATACAC AAAGCTCAAA AATGGCCACG TGTTTAGAAA GCAGGCGCTG
    CTGAGCCAGG AACGGGCTCT CCTGCATGAT GGGCTTGTCT ACTGGAAAAC CGCTACGGGT
    CGCTTCAAAG ACATCCTGGC TCTGCTACTA ACTGACGTGC TGCTCTTCTT ACAAGAAAAA
    GACCAGAAAT ACATCTTTGC AGCTGTTGAC CAGAAGCCAT CAGTGATTTC CCTCCAGAAG
    CTGATTGCTA GAGAAGTTGC CAACGAGGAG AGAGGCATGT TTCTGATCAG TGCTTCGTCA
    GCCGGCCCCG AGATGTACGA AATCCACACC AATTCCAAGG AGGAGCGGAA TAACTGGATG
    AGGCGGATCC AGCAGGCAGT AGAAAGTTGT CCAGAGGAAG AAGGAGGAAG GACGAGCGAG
    TCGGATGAAG AGAGGCGGAA AGCTGAAGCT AGAGTGGCCA AGATTCAGCA ATGTCAAGAA
    ATACTCAGTA ACCAAGACCA ACAGATTTGC ACGTACTTGG AAGAAAAGCT GCATATCTAC
    GCTGAACTTG GGGAACTGAG TGGCTTTGAG GATGTCCACC TAGAACCCCA TCTGCTCATC
    AAACCTGACC CTGGAGAGCC TCCCCAGGCA GCGTCCCTGC TTGCAGCAGC CCTGAGAGAA
    GCCGAGAGTC TGCAAGTGGC TGTGAAGGCT TCAAAGATGG GCGATGTAAG CCAGTCATCC
    GAAGAAAGCC CTGGAGGGAC TGTCCTGATG GACACACCCA GTACACAGGA TGTACCAGCG
    TCACCAACTG CTTCATTAGT CACAGAAGGG ACAGAAGGAA GAGGCTGTTG GGATGTGGAT
    CCCGGGCTCC AGGGTGTGGT AACCGACTTG GCAGTCTCTG ATGCAGGGGA GAAGGTGGAA
    TATAGAAGTT TCTCCGGTTC TTCACAGTCA GAGATTATAC AAGCAATACA GAATTTAACG
    CGTCTCCTCT ACAGCCTTCA GGCTGCCTTG ACCATCCAGG ACAGTCACAT TGAGATCCAC
    AAACTGGTTC TACAGCAGCG GGAGAGCTTG GCCCCTAGCC ACTCCTTCCG AGGTGGCCCC
    TTGCAAGACC AGGAGAAATC CCGCTACCTG GAAAAGCAGC GTGAAGAGCT GGCCAACATC
    CACAAGCTTC AACACCAGTT CCAGCAGGAG CAGCGGCGCT GGCACCGCAC GTGCGACCAG
    CAACAGCGCG AGCAGGAAGC CCAAGAGAGC TGGCTGCAGG CGCGGGAACG TGAGTGCCAG
    TCCCAGGAGG AATTGTTGCT GCGCCACCGT AGCGAGCTGG ACCACCAACT CCAGGAGTAC
    CAGCAGAGCC TTGAGCGGCT ACGGGAGGGC CAGCGGATGG TGGAGAGGGA GAGACAGAAG
    ATGCGTGTCC AGCAGGGTCT GCTGGGCCAC TGCAAGCATA GTCGCCAGCG GAGCCTCCCA
    GCCGTCTTCT CTCCAGGGAG CAAGGAGGTA ACAGAACTTA ACCGAGCTGA GAGTTTGTGT
    CATGAAAATT CATTTTTCAT CAATGAAGCT TTTGGACACA TGTCACTTAA CACGTCCAAT
    AAACCAAACC CATCAGGGGT CCCCTGGGAT GCTCACCCCC TGGAGGGGTC TCATTTTGAT
    TTGGCAAGGA CTAGTGAGAG TCCAACAGAA CTGAAGATAG ACATTTCTCA GCCTCCTGAT
    GTCAACTCTG AACTCTGGAC AACGGGGCCG GGCCATCAGA GACCTGCTCT CCAAGAAAAC
    AGCAAGGAGT CTTATAAAAA TGTTGCAGAT TTGGACTCCT TCCAGAGTGA ATCGTCATCT
    CCCCAGGACT CCAATCAGCG TGGCCCCCAG CCACAGACAC TCACAACAGA AGCAAAACTA
    AGTCTTCCGA TGGCGGCTGG ACACGGTGGC GATGCTGGAG ACGGGGCTGA AGAAAACATT
    CTTTACCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu48946
    Clone ID Related Accession (Same CDS sequence) XM_006517518.2 , XM_006517518.3
    Accession Version XM_006517518.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5145bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 28 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006517518.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGGCGGC GACGAGCCCG GCTGGCCAGA GATGCAGGAG CCATGGAGTT GAGCTGCAGT 
    GAAGTGCCTC TTTATGGACA GAAGACGGTC TATGCAAAGT TTGGCAAAAA CGTGTATCTA
    CCCGAAGATG CGGAGTTTTA CTTTGTTTAT GGCGGGTCGC ATCAGAGGCA CGTGGTGATT
    GCTGACCGTG TCCAGGACAA TGTCCTCCAA TCTAGCATTC CAGGCCACTG GCTTCAGGAG
    ACGGTGACGG TATCTGTGTG CCTCTGCTCG GAAGGGTACT CCCCAGTGAC CATGGGCTCT
    GGCTCAGTGA CCTACGTGGA CAACATGGCC TGCAGGCTGG CTCGTCTGCT GGTCACTCAG
    GCTGACCGAC TCACGGCCTG CAGTCACCAG ACGCTGCTGA CCCCGTTTGC CCTGACAGTG
    GAAGCGCTGC CTGCCTTGGA TGAGGAGCTC GTGCTGGCTC TGACCCAACT GGAGCTGCCT
    TTGGGTTGGA CAGTCCTGGG AAATTCCTCA CTTGAAGTAT CTCTACACAG AGAGTCTCTC
    CTGCATCTGG CTGTGAGATG GGCCCTCCCT AAGCTATTCC ACTTCCTCTT GTGTCTCCCT
    GGAGGAGTCA AGGCCTTGAA GCTACCTAAT GAAGAGGCCA CCACACCATT AGACTTAGCT
    TTGCAAGGTG GACACTCCAC ACTGGTAGAA GACATCACAA ATTTTCAGGG TAGCCATTCC
    CCAGGCTTCT CCCGACTGCG TCTTAACGAA GAGGCCACTC TGCAGTTTGT TCATTCATCG
    GAAACATTGA CCCTGACCGT TAACCACACA GCTGAGCATC TCTTGGAAGC AGACATCAAA
    CTCTTCCGGA AATATTTCTG GGACAGAGCC TTTCTTGTCA AGGCTCTTGA GCAAGAAGCC
    AAGACTGAGA AAGCAACCAT GCCTTCTGGA GCTGCAGAAA CTGAAGAAGA GGTTAGGAAT
    TTGGAGTCTG GCAGATCACC ATCGGAGGAG GAGGAGGATG CAAAGAGCAT CAAAAGCCAG
    GTGGATGGGC CGAGTGAACA CGAAGACCAG GACCGCCTAC CTCTGGATCG CTCCTTTGAT
    GGCCTCAAAA AATCCAAGCA TGTCCCAGCG TCCCTTGCTG CAGGCCAGCT CTCTGATGTT
    CTGAACGGGG GTGATGAAGT CTACGCTAAC TGTATGGTGA TTGACCAGGT TGGCGATTTG
    GATATCAACT ATATAAACCT AGAGGGACTC TCCACCCATA CGAGTCCCGA GTCTGGGAGG
    TCCATGCTGG GGCCTCAGGC TTGCATGCAC ACCCTTCCTC CCGACACCAG TCCCTGCGGG
    CGCCCACTCA TTGAGAACAG TGAGGGGACC CTGGATGCTG CAGCTTCGCA GTCTTTCGTG
    ACACCATCCA GTTCTCGCAC TTCCAACTTG AACCTTTCTT TTGGTCTCCA TGGATTTGAA
    AAGGAGCAGA GTCATCTCAA GAAAAGGAGT TCTAGCCTGG ATGCCCTGGT TGCCGACAGT
    GAGGGGGAAG GAGGTTCTGA GCCCCCCATC TGCTATGCTG TGGGCTCTCA GAGTTCCCCA
    AGAACTGGAC TCCCCAGCGG GGATGAACTG GACTCTTTTG AGACCAACAC TGAACCAGAC
    TGTAATATCT CCAGGACCGA ATCACTCTCT CTGTCCAGTA CTCTGCATTC AAAGGAGTCC
    TTGCTTTCTG GGATCCGGTC TCGCTCCTAC TCCTGCTCAT CACCCAAAAT CTCTTCAGGA
    AAATCCCGGC TAGTGCGCGA CTTCACAGTG TGCAGTACCA GCGAAGAACA GAGATCATAT
    AGTTTCCAGG AGCCACCAGG AGAAAAAAGG ATTCAGGAAG AAGAATGGGA TGAATACGTC
    ATTCCTGCCA AATCCGAGTC TGAAAAGTAC AAAGTGAGCC GAACTTTCAG CTTCCTCATG
    AACAGGATGA CCAGCCCACG GAACAAATCT AAGATGAAAA ACAAGGACAC TAAAGAAAAA
    GAAAAAATGA ATCGACATCA GTTTGTCCCG GGAACGTTCT CCGGGGTTCT GCAGTGCTCC
    GGTTGTGATA AAACACTCCT GGGGAAAGAG TCACTCCAGT GTGCCAACTG TAAGGCAAAC
    ACACACAAGG GCTGCAAAGA TGCTGTGCCT CCGTGTACCA AGAAATTCCA AGAGAAATAC
    AACAAGAACA AACCACAGAG CATCCTTGGA AGCTCTTCAG TCAGAGACGT CCCAGCACCT
    GGGCTCTCCT TGCACCCTTC TTCCTCTATG CCCATCGGAC TGCCAGCTGG AAGGAAGGAA
    TTTGCGGCTC AGGTGCATCC ACTGTCCAGA AGTGTCCCCG GCACCACTTT GGAAAGCTTC
    AGGAGGGCAG TGACATCGTT AGAGTCTGAG GGTGACAGCT GGAGAAGCAG GTCTCATTCC
    GATGAGCTGT TCCAGTCTAT GGGCTCTTCT CCTTCCACGG AGTCATTCAT GATGGAAGAT
    GTTGTGGATT CTTCTCTGTG GATCGACCTC AGTAGTGATG CTCAGGAGTT TGAAGCAGAG
    TCTTGGAGTC TCGTGGTGGA CCCTTCCTTT TGCAGTAGGC AGGAGAAGGA TGTCATCAAA
    AGACAAGATG TCATTTTTGA GCTGATGCAG ACAGAGGTCC ACCACATCCA GACTCTGCTC
    ATCATGTCCG AGGTCTTCAG GAAAGGCATG AAGGAGGAAC TGCAGCTGGA CCACAGCACG
    GTGGACAAGA TCTTCCCCTG CTTAGATGAG CTGCTGGAGA CTCACCGGCA CTTCTTCTTC
    AGCATGAAGG AGCGCAGGCA GGAGTCCTGC GCTGGCAGTG ACAGGAACTT TGTGATCAAC
    CAAATTGGAG ACATTCTGGT ACAACAGTTT TCAGAGGAAA ATGCAAGCAA AATGAAGAGG
    ATCTATGGAG AATTCTGCAG CCATCACAAA GAAGCCATGA GTCTCTTTAA AGAGCTGCAG
    CAGAACAAGA AGTTTCAGAA TTTTATTAAG ATCCGAAATA GCAACCTGTT GGCTCGTCGC
    CGCGGAATCC CGGAGTGCAT CCTGCTAGTT ACCCAGCGCA TCACAAAGTA CCCAGTCTTG
    GTGGAAAGGA TCCTGCAGTA CACCAAGGAA AGGACTGAAG AGCACCGGGA CCTGTGCAAA
    GCTCTCGGCT TAATTAAAGA CATGATCGCA GCCGTGGACC TGAAAGTCAG CGAATACGAG
    AAGAACCAAA AATGGCTCGA GATCCTAAAT AAAATCGAGA ACAAAACATA CACAAAGCTC
    AAAAATGGCC ACGTGTTTAG AAAGCAGGCG CTGCTGAGCC AGGAACGGGC TCTCCTGCAT
    GATGGGCTTG TCTACTGGAA AACCGCTACG GGTCGCTTCA AAGACATCCT GGCTCTGCTA
    CTAACTGACG TGCTGCTCTT CTTACAAGAA AAAGACCAGA AATACATCTT TGCAGCTGTT
    GACCAGAAGC CATCAGTGAT TTCCCTCCAG AAGCTGATTG CTAGAGAAGT TGCCAACGAG
    GAGAGAGGCA TGTTTCTGAT CAGTGCTTCG TCAGCCGGCC CCGAGATGTA CGAAATCCAC
    ACCAATTCCA AGGAGGAGCG GAATAACTGG ATGAGGCGGA TCCAGCAGGC AGTAGAAAGT
    TGTCCAGAGG AAGAAGGAGG AAGGACGAGC GAGTCGGATG AAGAGAGGCG GAAAGCTGAA
    GCTAGAGTGG CCAAGATTCA GCAATGTCAA GAAATACTCA GTAACCAAGA CCAACAGATT
    TGCACGTACT TGGAAGAAAA GCTGCATATC TACGCTGAAC TTGGGGAACT GAGTGGCTTT
    GAGGATGTCC ACCTAGAACC CCATCTGCTC ATCAAACCTG ACCCTGGAGA GCCTCCCCAG
    GCAGCGTCCC TGCTTGCAGC AGCCCTGAGA GAAGCCGAGA GTCTGCAAGT GGCTGTGAAG
    GCTTCAAAGA TGGGCGATGT AAGCCAGTCA TCCGAAGAAA GCCCTGGAGG GACTGTCCTG
    ATGGACACAC CCAGTACACA GGATGTACCA GCGTCACCAA CTGCTTCATT AGTCACAGAA
    GGGACAGAAG GAAGAGGCTG TTGGGATGTG GATCCCGGGC TCCAGGGTGT GGTAACCGAC
    TTGGCAGTCT CTGATGCAGG GGAGAAGGTG GAATATAGAA GTTTCTCCGG TTCTTCACAG
    TCAGAGATTA TACAAGCAAT ACAGAATTTA ACGCGTCTCC TCTACAGCCT TCAGGCTGCC
    TTGACCATCC AGGACAGTCA CATTGAGATC CACAAACTGG TTCTACAGCA GCGGGAGAGC
    TTGGCCCCTA GCCACTCCTT CCGAGGTGGC CCCTTGCAAG ACCAGGAGAA ATCCCGCTAC
    CTGGAAAAGC AGCGTGAAGA GCTGGCCAAC ATCCACAAGC TTCAACACCA GTTCCAGCAG
    GAGCAGCGGC GCTGGCACCG CACGTGCGAC CAGCAACAGC GCGAGCAGGA AGCCCAAGAG
    AGCTGGCTGC AGGCGCGGGA ACGTGAGTGC CAGTCCCAGG AGGAATTGTT GCTGCGCCAC
    CGTAGCGAGC TGGACCACCA ACTCCAGGAG TACCAGCAGA GCCTTGAGCG GCTACGGGAG
    GGCCAGCGGA TGGTGGAGAG GGAGAGACAG AAGATGCGTG TCCAGCAGGG TCTGCTGGGC
    CACTGCAAGC ATAGTCGCCA GCGGAGCCTC CCAGCCGTCT TCTCTCCAGG GAGCAAGGAG
    GTAACAGAAC TTAACCGAGC TGAGAGTTTG TGTCATGAAA ATTCATTTTT CATCAATGAA
    GCTTTTGGAC ACATGTCACT TAACACGTCC AATAAACCAA ACCCATCAGG GGTCCCCTGG
    GATGCTCACC CCCTGGAGGG GTCTCATTTT GATTTGGCAA GGACTAGTGA GAGTCCAACA
    GAACTGAAGA TAGACATTTC TCAGCCTCCT GATGTCAACT CTGAACTCTG GACAACGGGG
    CCGGGCCATC AGAGACCTGC TCTCCAAGAA AACAGCAAGG AGTCTTATAA AAATGTTGCA
    GATTTGGACT CCTTCCAGAG TGAATCGTCA TCTCCCCAGG ACTCCAATCA GCGTGGCCCC
    CAGCCACAGA CACTCACAAC AGAAGCAAAA CTAAGTCTTC CGATGGCGGC TGGACACGGT
    GGCGATGCTG GAGACGGGGC TGAAGAAAAC ATTCTTTACC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006517581.1
    CDS322..5466
    Translation

    Target ORF information:

    RefSeq Version XM_006517518.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006517518.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGGCGGC GACGAGCCCG GCTGGCCAGA GATGCAGGAG CCATGGAGTT GAGCTGCAGT 
    GAAGTGCCTC TTTATGGACA GAAGACGGTC TATGCAAAGT TTGGCAAAAA CGTGTATCTA
    CCCGAAGATG CGGAGTTTTA CTTTGTTTAT GGCGGGTCGC ATCAGAGGCA CGTGGTGATT
    GCTGACCGTG TCCAGGACAA TGTCCTCCAA TCTAGCATTC CAGGCCACTG GCTTCAGGAG
    ACGGTGACGG TATCTGTGTG CCTCTGCTCG GAAGGGTACT CCCCAGTGAC CATGGGCTCT
    GGCTCAGTGA CCTACGTGGA CAACATGGCC TGCAGGCTGG CTCGTCTGCT GGTCACTCAG
    GCTGACCGAC TCACGGCCTG CAGTCACCAG ACGCTGCTGA CCCCGTTTGC CCTGACAGTG
    GAAGCGCTGC CTGCCTTGGA TGAGGAGCTC GTGCTGGCTC TGACCCAACT GGAGCTGCCT
    TTGGGTTGGA CAGTCCTGGG AAATTCCTCA CTTGAAGTAT CTCTACACAG AGAGTCTCTC
    CTGCATCTGG CTGTGAGATG GGCCCTCCCT AAGCTATTCC ACTTCCTCTT GTGTCTCCCT
    GGAGGAGTCA AGGCCTTGAA GCTACCTAAT GAAGAGGCCA CCACACCATT AGACTTAGCT
    TTGCAAGGTG GACACTCCAC ACTGGTAGAA GACATCACAA ATTTTCAGGG TAGCCATTCC
    CCAGGCTTCT CCCGACTGCG TCTTAACGAA GAGGCCACTC TGCAGTTTGT TCATTCATCG
    GAAACATTGA CCCTGACCGT TAACCACACA GCTGAGCATC TCTTGGAAGC AGACATCAAA
    CTCTTCCGGA AATATTTCTG GGACAGAGCC TTTCTTGTCA AGGCTCTTGA GCAAGAAGCC
    AAGACTGAGA AAGCAACCAT GCCTTCTGGA GCTGCAGAAA CTGAAGAAGA GGTTAGGAAT
    TTGGAGTCTG GCAGATCACC ATCGGAGGAG GAGGAGGATG CAAAGAGCAT CAAAAGCCAG
    GTGGATGGGC CGAGTGAACA CGAAGACCAG GACCGCCTAC CTCTGGATCG CTCCTTTGAT
    GGCCTCAAAA AATCCAAGCA TGTCCCAGCG TCCCTTGCTG CAGGCCAGCT CTCTGATGTT
    CTGAACGGGG GTGATGAAGT CTACGCTAAC TGTATGGTGA TTGACCAGGT TGGCGATTTG
    GATATCAACT ATATAAACCT AGAGGGACTC TCCACCCATA CGAGTCCCGA GTCTGGGAGG
    TCCATGCTGG GGCCTCAGGC TTGCATGCAC ACCCTTCCTC CCGACACCAG TCCCTGCGGG
    CGCCCACTCA TTGAGAACAG TGAGGGGACC CTGGATGCTG CAGCTTCGCA GTCTTTCGTG
    ACACCATCCA GTTCTCGCAC TTCCAACTTG AACCTTTCTT TTGGTCTCCA TGGATTTGAA
    AAGGAGCAGA GTCATCTCAA GAAAAGGAGT TCTAGCCTGG ATGCCCTGGT TGCCGACAGT
    GAGGGGGAAG GAGGTTCTGA GCCCCCCATC TGCTATGCTG TGGGCTCTCA GAGTTCCCCA
    AGAACTGGAC TCCCCAGCGG GGATGAACTG GACTCTTTTG AGACCAACAC TGAACCAGAC
    TGTAATATCT CCAGGACCGA ATCACTCTCT CTGTCCAGTA CTCTGCATTC AAAGGAGTCC
    TTGCTTTCTG GGATCCGGTC TCGCTCCTAC TCCTGCTCAT CACCCAAAAT CTCTTCAGGA
    AAATCCCGGC TAGTGCGCGA CTTCACAGTG TGCAGTACCA GCGAAGAACA GAGATCATAT
    AGTTTCCAGG AGCCACCAGG AGAAAAAAGG ATTCAGGAAG AAGAATGGGA TGAATACGTC
    ATTCCTGCCA AATCCGAGTC TGAAAAGTAC AAAGTGAGCC GAACTTTCAG CTTCCTCATG
    AACAGGATGA CCAGCCCACG GAACAAATCT AAGATGAAAA ACAAGGACAC TAAAGAAAAA
    GAAAAAATGA ATCGACATCA GTTTGTCCCG GGAACGTTCT CCGGGGTTCT GCAGTGCTCC
    GGTTGTGATA AAACACTCCT GGGGAAAGAG TCACTCCAGT GTGCCAACTG TAAGGCAAAC
    ACACACAAGG GCTGCAAAGA TGCTGTGCCT CCGTGTACCA AGAAATTCCA AGAGAAATAC
    AACAAGAACA AACCACAGAG CATCCTTGGA AGCTCTTCAG TCAGAGACGT CCCAGCACCT
    GGGCTCTCCT TGCACCCTTC TTCCTCTATG CCCATCGGAC TGCCAGCTGG AAGGAAGGAA
    TTTGCGGCTC AGGTGCATCC ACTGTCCAGA AGTGTCCCCG GCACCACTTT GGAAAGCTTC
    AGGAGGGCAG TGACATCGTT AGAGTCTGAG GGTGACAGCT GGAGAAGCAG GTCTCATTCC
    GATGAGCTGT TCCAGTCTAT GGGCTCTTCT CCTTCCACGG AGTCATTCAT GATGGAAGAT
    GTTGTGGATT CTTCTCTGTG GATCGACCTC AGTAGTGATG CTCAGGAGTT TGAAGCAGAG
    TCTTGGAGTC TCGTGGTGGA CCCTTCCTTT TGCAGTAGGC AGGAGAAGGA TGTCATCAAA
    AGACAAGATG TCATTTTTGA GCTGATGCAG ACAGAGGTCC ACCACATCCA GACTCTGCTC
    ATCATGTCCG AGGTCTTCAG GAAAGGCATG AAGGAGGAAC TGCAGCTGGA CCACAGCACG
    GTGGACAAGA TCTTCCCCTG CTTAGATGAG CTGCTGGAGA CTCACCGGCA CTTCTTCTTC
    AGCATGAAGG AGCGCAGGCA GGAGTCCTGC GCTGGCAGTG ACAGGAACTT TGTGATCAAC
    CAAATTGGAG ACATTCTGGT ACAACAGTTT TCAGAGGAAA ATGCAAGCAA AATGAAGAGG
    ATCTATGGAG AATTCTGCAG CCATCACAAA GAAGCCATGA GTCTCTTTAA AGAGCTGCAG
    CAGAACAAGA AGTTTCAGAA TTTTATTAAG ATCCGAAATA GCAACCTGTT GGCTCGTCGC
    CGCGGAATCC CGGAGTGCAT CCTGCTAGTT ACCCAGCGCA TCACAAAGTA CCCAGTCTTG
    GTGGAAAGGA TCCTGCAGTA CACCAAGGAA AGGACTGAAG AGCACCGGGA CCTGTGCAAA
    GCTCTCGGCT TAATTAAAGA CATGATCGCA GCCGTGGACC TGAAAGTCAG CGAATACGAG
    AAGAACCAAA AATGGCTCGA GATCCTAAAT AAAATCGAGA ACAAAACATA CACAAAGCTC
    AAAAATGGCC ACGTGTTTAG AAAGCAGGCG CTGCTGAGCC AGGAACGGGC TCTCCTGCAT
    GATGGGCTTG TCTACTGGAA AACCGCTACG GGTCGCTTCA AAGACATCCT GGCTCTGCTA
    CTAACTGACG TGCTGCTCTT CTTACAAGAA AAAGACCAGA AATACATCTT TGCAGCTGTT
    GACCAGAAGC CATCAGTGAT TTCCCTCCAG AAGCTGATTG CTAGAGAAGT TGCCAACGAG
    GAGAGAGGCA TGTTTCTGAT CAGTGCTTCG TCAGCCGGCC CCGAGATGTA CGAAATCCAC
    ACCAATTCCA AGGAGGAGCG GAATAACTGG ATGAGGCGGA TCCAGCAGGC AGTAGAAAGT
    TGTCCAGAGG AAGAAGGAGG AAGGACGAGC GAGTCGGATG AAGAGAGGCG GAAAGCTGAA
    GCTAGAGTGG CCAAGATTCA GCAATGTCAA GAAATACTCA GTAACCAAGA CCAACAGATT
    TGCACGTACT TGGAAGAAAA GCTGCATATC TACGCTGAAC TTGGGGAACT GAGTGGCTTT
    GAGGATGTCC ACCTAGAACC CCATCTGCTC ATCAAACCTG ACCCTGGAGA GCCTCCCCAG
    GCAGCGTCCC TGCTTGCAGC AGCCCTGAGA GAAGCCGAGA GTCTGCAAGT GGCTGTGAAG
    GCTTCAAAGA TGGGCGATGT AAGCCAGTCA TCCGAAGAAA GCCCTGGAGG GACTGTCCTG
    ATGGACACAC CCAGTACACA GGATGTACCA GCGTCACCAA CTGCTTCATT AGTCACAGAA
    GGGACAGAAG GAAGAGGCTG TTGGGATGTG GATCCCGGGC TCCAGGGTGT GGTAACCGAC
    TTGGCAGTCT CTGATGCAGG GGAGAAGGTG GAATATAGAA GTTTCTCCGG TTCTTCACAG
    TCAGAGATTA TACAAGCAAT ACAGAATTTA ACGCGTCTCC TCTACAGCCT TCAGGCTGCC
    TTGACCATCC AGGACAGTCA CATTGAGATC CACAAACTGG TTCTACAGCA GCGGGAGAGC
    TTGGCCCCTA GCCACTCCTT CCGAGGTGGC CCCTTGCAAG ACCAGGAGAA ATCCCGCTAC
    CTGGAAAAGC AGCGTGAAGA GCTGGCCAAC ATCCACAAGC TTCAACACCA GTTCCAGCAG
    GAGCAGCGGC GCTGGCACCG CACGTGCGAC CAGCAACAGC GCGAGCAGGA AGCCCAAGAG
    AGCTGGCTGC AGGCGCGGGA ACGTGAGTGC CAGTCCCAGG AGGAATTGTT GCTGCGCCAC
    CGTAGCGAGC TGGACCACCA ACTCCAGGAG TACCAGCAGA GCCTTGAGCG GCTACGGGAG
    GGCCAGCGGA TGGTGGAGAG GGAGAGACAG AAGATGCGTG TCCAGCAGGG TCTGCTGGGC
    CACTGCAAGC ATAGTCGCCA GCGGAGCCTC CCAGCCGTCT TCTCTCCAGG GAGCAAGGAG
    GTAACAGAAC TTAACCGAGC TGAGAGTTTG TGTCATGAAA ATTCATTTTT CATCAATGAA
    GCTTTTGGAC ACATGTCACT TAACACGTCC AATAAACCAA ACCCATCAGG GGTCCCCTGG
    GATGCTCACC CCCTGGAGGG GTCTCATTTT GATTTGGCAA GGACTAGTGA GAGTCCAACA
    GAACTGAAGA TAGACATTTC TCAGCCTCCT GATGTCAACT CTGAACTCTG GACAACGGGG
    CCGGGCCATC AGAGACCTGC TCTCCAAGAA AACAGCAAGG AGTCTTATAA AAATGTTGCA
    GATTTGGACT CCTTCCAGAG TGAATCGTCA TCTCCCCAGG ACTCCAATCA GCGTGGCCCC
    CAGCCACAGA CACTCACAAC AGAAGCAAAA CTAAGTCTTC CGATGGCGGC TGGACACGGT
    GGCGATGCTG GAGACGGGGC TGAAGAAAAC ATTCTTTACC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu78409
    Clone ID Related Accession (Same CDS sequence) XM_017315361.1 , XM_017315361.2
    Accession Version XM_017315361.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4854bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 28 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGCTCTG GCTCAGTGAC CTACGTGGAC AACATGGCCT GCAGGCTGGC TCGTCTGCTG 
    GTCACTCAGG CTGACCGACT CACGGCCTGC AGTCACCAGA CGCTGCTGAC CCCGTTTGCC
    CTGACAGTGG AAGCGCTGCC TGCCTTGGAT GAGGAGCTCG TGCTGGCTCT GACCCAACTG
    GAGCTGCCTT TGGGTTGGAC AGTCCTGGGA AATTCCTCAC TTGAAGTATC TCTACACAGA
    GAGTCTCTCC TGCATCTGGC TGTGAGATGG GCCCTCCCTA AGCTATTCCA CTTCCTCTTG
    TGTCTCCCTG GAGGAGTCAA GGCCTTGAAG CTACCTAATG AAGAGGCCAC CACACCATTA
    GACTTAGCTT TGCAAGGTGG ACACTCCACA CTGGTAGAAG ACATCACAAA TTTTCAGGGT
    AGCCATTCCC CAGGCTTCTC CCGACTGCGT CTTAACGAAG AGGCCACTCT GCAGTTTGTT
    CATTCATCGG AAACATTGAC CCTGACCGTT AACCACACAG CTGAGCATCT CTTGGAAGCA
    GACATCAAAC TCTTCCGGAA ATATTTCTGG GACAGAGCCT TTCTTGTCAA GGCTCTTGAG
    CAAGAAGCCA AGACTGAGAA AGCAACCATG CCTTCTGGAG CTGCAGAAAC TGAAGAAGAG
    GTTAGGAATT TGGAGTCTGG CAGATCACCA TCGGAGGAGG AGGAGGATGC AAAGAGCATC
    AAAAGCCAGG TGGATGGGCC GAGTGAACAC GAAGACCAGG ACCGCCTACC TCTGGATCGC
    TCCTTTGATG GCCTCAAAAA ATCCAAGCAT GTCCCAGCGT CCCTTGCTGC AGGCCAGCTC
    TCTGATGTTC TGAACGGGGG TGATGAAGTC TACGCTAACT GTATGGTGAT TGACCAGGTT
    GGCGATTTGG ATATCAACTA TATAAACCTA GAGGGACTCT CCACCCATAC GAGTCCCGAG
    TCTGGGAGGT CCATGCTGGG GCCTCAGGCT TGCATGCACA CCCTTCCTCC CGACACCAGT
    CCCTGCGGGC GCCCACTCAT TGAGAACAGT GAGGGGACCC TGGATGCTGC AGCTTCGCAG
    TCTTTCGTGA CACCATCCAG TTCTCGCACT TCCAACTTGA ACCTTTCTTT TGGTCTCCAT
    GGATTTGAAA AGGAGCAGAG TCATCTCAAG AAAAGGAGTT CTAGCCTGGA TGCCCTGGTT
    GCCGACAGTG AGGGGGAAGG AGGTTCTGAG CCCCCCATCT GCTATGCTGT GGGCTCTCAG
    AGTTCCCCAA GAACTGGACT CCCCAGCGGG GATGAACTGG ACTCTTTTGA GACCAACACT
    GAACCAGACT GTAATATCTC CAGGACCGAA TCACTCTCTC TGTCCAGTAC TCTGCATTCA
    AAGGAGTCCT TGCTTTCTGG GATCCGGTCT CGCTCCTACT CCTGCTCATC ACCCAAAATC
    TCTTCAGGAA AATCCCGGCT AGTGCGCGAC TTCACAGTGT GCAGTACCAG CGAAGAACAG
    AGATCATATA GTTTCCAGGA GCCACCAGGA GAAAAAAGGA TTCAGGAAGA AGAATGGGAT
    GAATACGTCA TTCCTGCCAA ATCCGAGTCT GAAAAGTACA AAGTGAGCCG AACTTTCAGC
    TTCCTCATGA ACAGGATGAC CAGCCCACGG AACAAATCTA AGATGAAAAA CAAGGACACT
    AAAGAAAAAG AAAAAATGAA TCGACATCAG TTTGTCCCGG GAACGTTCTC CGGGGTTCTG
    CAGTGCTCCG GTTGTGATAA AACACTCCTG GGGAAAGAGT CACTCCAGTG TGCCAACTGT
    AAGGCAAACA CACACAAGGG CTGCAAAGAT GCTGTGCCTC CGTGTACCAA GAAATTCCAA
    GAGAAATACA ACAAGAACAA ACCACAGAGC ATCCTTGGAA GCTCTTCAGT CAGAGACGTC
    CCAGCACCTG GGCTCTCCTT GCACCCTTCT TCCTCTATGC CCATCGGACT GCCAGCTGGA
    AGGAAGGAAT TTGCGGCTCA GGTGCATCCA CTGTCCAGAA GTGTCCCCGG CACCACTTTG
    GAAAGCTTCA GGAGGGCAGT GACATCGTTA GAGTCTGAGG GTGACAGCTG GAGAAGCAGG
    TCTCATTCCG ATGAGCTGTT CCAGTCTATG GGCTCTTCTC CTTCCACGGA GTCATTCATG
    ATGGAAGATG TTGTGGATTC TTCTCTGTGG ATCGACCTCA GTAGTGATGC TCAGGAGTTT
    GAAGCAGAGT CTTGGAGTCT CGTGGTGGAC CCTTCCTTTT GCAGTAGGCA GGAGAAGGAT
    GTCATCAAAA GACAAGATGT CATTTTTGAG CTGATGCAGA CAGAGGTCCA CCACATCCAG
    ACTCTGCTCA TCATGTCCGA GGTCTTCAGG AAAGGCATGA AGGAGGAACT GCAGCTGGAC
    CACAGCACGG TGGACAAGAT CTTCCCCTGC TTAGATGAGC TGCTGGAGAC TCACCGGCAC
    TTCTTCTTCA GCATGAAGGA GCGCAGGCAG GAGTCCTGCG CTGGCAGTGA CAGGAACTTT
    GTGATCAACC AAATTGGAGA CATTCTGGTA CAACAGTTTT CAGAGGAAAA TGCAAGCAAA
    ATGAAGAGGA TCTATGGAGA ATTCTGCAGC CATCACAAAG AAGCCATGAG TCTCTTTAAA
    GAGCTGCAGC AGAACAAGAA GTTTCAGAAT TTTATTAAGA TCCGAAATAG CAACCTGTTG
    GCTCGTCGCC GCGGAATCCC GGAGTGCATC CTGCTAGTTA CCCAGCGCAT CACAAAGTAC
    CCAGTCTTGG TGGAAAGGAT CCTGCAGTAC ACCAAGGAAA GGACTGAAGA GCACCGGGAC
    CTGTGCAAAG CTCTCGGCTT AATTAAAGAC ATGATCGCAG CCGTGGACCT GAAAGTCAGC
    GAATACGAGA AGAACCAAAA ATGGCTCGAG ATCCTAAATA AAATCGAGAA CAAAACATAC
    ACAAAGCTCA AAAATGGCCA CGTGTTTAGA AAGCAGGCGC TGCTGAGCCA GGAACGGGCT
    CTCCTGCATG ATGGGCTTGT CTACTGGAAA ACCGCTACGG GTCGCTTCAA AGACATCCTG
    GCTCTGCTAC TAACTGACGT GCTGCTCTTC TTACAAGAAA AAGACCAGAA ATACATCTTT
    GCAGCTGTTG ACCAGAAGCC ATCAGTGATT TCCCTCCAGA AGCTGATTGC TAGAGAAGTT
    GCCAACGAGG AGAGAGGCAT GTTTCTGATC AGTGCTTCGT CAGCCGGCCC CGAGATGTAC
    GAAATCCACA CCAATTCCAA GGAGGAGCGG AATAACTGGA TGAGGCGGAT CCAGCAGGCA
    GTAGAAAGTT GTCCAGAGGA AGAAGGAGGA AGGACGAGCG AGTCGGATGA AGAGAGGCGG
    AAAGCTGAAG CTAGAGTGGC CAAGATTCAG CAATGTCAAG AAATACTCAG TAACCAAGAC
    CAACAGATTT GCACGTACTT GGAAGAAAAG CTGCATATCT ACGCTGAACT TGGGGAACTG
    AGTGGCTTTG AGGATGTCCA CCTAGAACCC CATCTGCTCA TCAAACCTGA CCCTGGAGAG
    CCTCCCCAGG CAGCGTCCCT GCTTGCAGCA GCCCTGAGAG AAGCCGAGAG TCTGCAAGTG
    GCTGTGAAGG CTTCAAAGAT GGGCGATGTA AGCCAGTCAT CCGAAGAAAG CCCTGGAGGG
    ACTGTCCTGA TGGACACACC CAGTACACAG GATGTACCAG CGTCACCAAC TGCTTCATTA
    GTCACAGAAG GGACAGAAGG AAGAGGCTGT TGGGATGTGG ATCCCGGGCT CCAGGGTGTG
    GTAACCGACT TGGCAGTCTC TGATGCAGGG GAGAAGGTGG AATATAGAAG TTTCTCCGGT
    TCTTCACAGT CAGAGATTAT ACAAGCAATA CAGAATTTAA CGCGTCTCCT CTACAGCCTT
    CAGGCTGCCT TGACCATCCA GGACAGTCAC ATTGAGATCC ACAAACTGGT TCTACAGCAG
    CGGGAGAGCT TGGCCCCTAG CCACTCCTTC CGAGGTGGCC CCTTGCAAGA CCAGGAGAAA
    TCCCGCTACC TGGAAAAGCA GCGTGAAGAG CTGGCCAACA TCCACAAGCT TCAACACCAG
    TTCCAGCAGG AGCAGCGGCG CTGGCACCGC ACGTGCGACC AGCAACAGCG CGAGCAGGAA
    GCCCAAGAGA GCTGGCTGCA GGCGCGGGAA CGTGAGTGCC AGTCCCAGGA GGAATTGTTG
    CTGCGCCACC GTAGCGAGCT GGACCACCAA CTCCAGGAGT ACCAGCAGAG CCTTGAGCGG
    CTACGGGAGG GCCAGCGGAT GGTGGAGAGG GAGAGACAGA AGATGCGTGT CCAGCAGGGT
    CTGCTGGGCC ACTGCAAGCA TAGTCGCCAG CGGAGCCTCC CAGCCGTCTT CTCTCCAGGG
    AGCAAGGAGG TAACAGAACT TAACCGAGCT GAGAGTTTGT GTCATGAAAA TTCATTTTTC
    ATCAATGAAG CTTTTGGACA CATGTCACTT AACACGTCCA ATAAACCAAA CCCATCAGGG
    GTCCCCTGGG ATGCTCACCC CCTGGAGGGG TCTCATTTTG ATTTGGCAAG GACTAGTGAG
    AGTCCAACAG AACTGAAGAT AGACATTTCT CAGCCTCCTG ATGTCAACTC TGAACTCTGG
    ACAACGGGGC CGGGCCATCA GAGACCTGCT CTCCAAGAAA ACAGCAAGGA GTCTTATAAA
    AATGTTGCAG ATTTGGACTC CTTCCAGAGT GAATCGTCAT CTCCCCAGGA CTCCAATCAG
    CGTGGCCCCC AGCCACAGAC ACTCACAACA GAAGCAAAAC TAAGTCTTCC GATGGCGGCT
    GGACACGGTG GCGATGCTGG AGACGGGGCT GAAGAAAACA TTCTTTACCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170850.1
    CDS236..5089
    Translation

    Target ORF information:

    RefSeq Version XM_017315361.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017315361.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGCTCTG GCTCAGTGAC CTACGTGGAC AACATGGCCT GCAGGCTGGC TCGTCTGCTG 
    GTCACTCAGG CTGACCGACT CACGGCCTGC AGTCACCAGA CGCTGCTGAC CCCGTTTGCC
    CTGACAGTGG AAGCGCTGCC TGCCTTGGAT GAGGAGCTCG TGCTGGCTCT GACCCAACTG
    GAGCTGCCTT TGGGTTGGAC AGTCCTGGGA AATTCCTCAC TTGAAGTATC TCTACACAGA
    GAGTCTCTCC TGCATCTGGC TGTGAGATGG GCCCTCCCTA AGCTATTCCA CTTCCTCTTG
    TGTCTCCCTG GAGGAGTCAA GGCCTTGAAG CTACCTAATG AAGAGGCCAC CACACCATTA
    GACTTAGCTT TGCAAGGTGG ACACTCCACA CTGGTAGAAG ACATCACAAA TTTTCAGGGT
    AGCCATTCCC CAGGCTTCTC CCGACTGCGT CTTAACGAAG AGGCCACTCT GCAGTTTGTT
    CATTCATCGG AAACATTGAC CCTGACCGTT AACCACACAG CTGAGCATCT CTTGGAAGCA
    GACATCAAAC TCTTCCGGAA ATATTTCTGG GACAGAGCCT TTCTTGTCAA GGCTCTTGAG
    CAAGAAGCCA AGACTGAGAA AGCAACCATG CCTTCTGGAG CTGCAGAAAC TGAAGAAGAG
    GTTAGGAATT TGGAGTCTGG CAGATCACCA TCGGAGGAGG AGGAGGATGC AAAGAGCATC
    AAAAGCCAGG TGGATGGGCC GAGTGAACAC GAAGACCAGG ACCGCCTACC TCTGGATCGC
    TCCTTTGATG GCCTCAAAAA ATCCAAGCAT GTCCCAGCGT CCCTTGCTGC AGGCCAGCTC
    TCTGATGTTC TGAACGGGGG TGATGAAGTC TACGCTAACT GTATGGTGAT TGACCAGGTT
    GGCGATTTGG ATATCAACTA TATAAACCTA GAGGGACTCT CCACCCATAC GAGTCCCGAG
    TCTGGGAGGT CCATGCTGGG GCCTCAGGCT TGCATGCACA CCCTTCCTCC CGACACCAGT
    CCCTGCGGGC GCCCACTCAT TGAGAACAGT GAGGGGACCC TGGATGCTGC AGCTTCGCAG
    TCTTTCGTGA CACCATCCAG TTCTCGCACT TCCAACTTGA ACCTTTCTTT TGGTCTCCAT
    GGATTTGAAA AGGAGCAGAG TCATCTCAAG AAAAGGAGTT CTAGCCTGGA TGCCCTGGTT
    GCCGACAGTG AGGGGGAAGG AGGTTCTGAG CCCCCCATCT GCTATGCTGT GGGCTCTCAG
    AGTTCCCCAA GAACTGGACT CCCCAGCGGG GATGAACTGG ACTCTTTTGA GACCAACACT
    GAACCAGACT GTAATATCTC CAGGACCGAA TCACTCTCTC TGTCCAGTAC TCTGCATTCA
    AAGGAGTCCT TGCTTTCTGG GATCCGGTCT CGCTCCTACT CCTGCTCATC ACCCAAAATC
    TCTTCAGGAA AATCCCGGCT AGTGCGCGAC TTCACAGTGT GCAGTACCAG CGAAGAACAG
    AGATCATATA GTTTCCAGGA GCCACCAGGA GAAAAAAGGA TTCAGGAAGA AGAATGGGAT
    GAATACGTCA TTCCTGCCAA ATCCGAGTCT GAAAAGTACA AAGTGAGCCG AACTTTCAGC
    TTCCTCATGA ACAGGATGAC CAGCCCACGG AACAAATCTA AGATGAAAAA CAAGGACACT
    AAAGAAAAAG AAAAAATGAA TCGACATCAG TTTGTCCCGG GAACGTTCTC CGGGGTTCTG
    CAGTGCTCCG GTTGTGATAA AACACTCCTG GGGAAAGAGT CACTCCAGTG TGCCAACTGT
    AAGGCAAACA CACACAAGGG CTGCAAAGAT GCTGTGCCTC CGTGTACCAA GAAATTCCAA
    GAGAAATACA ACAAGAACAA ACCACAGAGC ATCCTTGGAA GCTCTTCAGT CAGAGACGTC
    CCAGCACCTG GGCTCTCCTT GCACCCTTCT TCCTCTATGC CCATCGGACT GCCAGCTGGA
    AGGAAGGAAT TTGCGGCTCA GGTGCATCCA CTGTCCAGAA GTGTCCCCGG CACCACTTTG
    GAAAGCTTCA GGAGGGCAGT GACATCGTTA GAGTCTGAGG GTGACAGCTG GAGAAGCAGG
    TCTCATTCCG ATGAGCTGTT CCAGTCTATG GGCTCTTCTC CTTCCACGGA GTCATTCATG
    ATGGAAGATG TTGTGGATTC TTCTCTGTGG ATCGACCTCA GTAGTGATGC TCAGGAGTTT
    GAAGCAGAGT CTTGGAGTCT CGTGGTGGAC CCTTCCTTTT GCAGTAGGCA GGAGAAGGAT
    GTCATCAAAA GACAAGATGT CATTTTTGAG CTGATGCAGA CAGAGGTCCA CCACATCCAG
    ACTCTGCTCA TCATGTCCGA GGTCTTCAGG AAAGGCATGA AGGAGGAACT GCAGCTGGAC
    CACAGCACGG TGGACAAGAT CTTCCCCTGC TTAGATGAGC TGCTGGAGAC TCACCGGCAC
    TTCTTCTTCA GCATGAAGGA GCGCAGGCAG GAGTCCTGCG CTGGCAGTGA CAGGAACTTT
    GTGATCAACC AAATTGGAGA CATTCTGGTA CAACAGTTTT CAGAGGAAAA TGCAAGCAAA
    ATGAAGAGGA TCTATGGAGA ATTCTGCAGC CATCACAAAG AAGCCATGAG TCTCTTTAAA
    GAGCTGCAGC AGAACAAGAA GTTTCAGAAT TTTATTAAGA TCCGAAATAG CAACCTGTTG
    GCTCGTCGCC GCGGAATCCC GGAGTGCATC CTGCTAGTTA CCCAGCGCAT CACAAAGTAC
    CCAGTCTTGG TGGAAAGGAT CCTGCAGTAC ACCAAGGAAA GGACTGAAGA GCACCGGGAC
    CTGTGCAAAG CTCTCGGCTT AATTAAAGAC ATGATCGCAG CCGTGGACCT GAAAGTCAGC
    GAATACGAGA AGAACCAAAA ATGGCTCGAG ATCCTAAATA AAATCGAGAA CAAAACATAC
    ACAAAGCTCA AAAATGGCCA CGTGTTTAGA AAGCAGGCGC TGCTGAGCCA GGAACGGGCT
    CTCCTGCATG ATGGGCTTGT CTACTGGAAA ACCGCTACGG GTCGCTTCAA AGACATCCTG
    GCTCTGCTAC TAACTGACGT GCTGCTCTTC TTACAAGAAA AAGACCAGAA ATACATCTTT
    GCAGCTGTTG ACCAGAAGCC ATCAGTGATT TCCCTCCAGA AGCTGATTGC TAGAGAAGTT
    GCCAACGAGG AGAGAGGCAT GTTTCTGATC AGTGCTTCGT CAGCCGGCCC CGAGATGTAC
    GAAATCCACA CCAATTCCAA GGAGGAGCGG AATAACTGGA TGAGGCGGAT CCAGCAGGCA
    GTAGAAAGTT GTCCAGAGGA AGAAGGAGGA AGGACGAGCG AGTCGGATGA AGAGAGGCGG
    AAAGCTGAAG CTAGAGTGGC CAAGATTCAG CAATGTCAAG AAATACTCAG TAACCAAGAC
    CAACAGATTT GCACGTACTT GGAAGAAAAG CTGCATATCT ACGCTGAACT TGGGGAACTG
    AGTGGCTTTG AGGATGTCCA CCTAGAACCC CATCTGCTCA TCAAACCTGA CCCTGGAGAG
    CCTCCCCAGG CAGCGTCCCT GCTTGCAGCA GCCCTGAGAG AAGCCGAGAG TCTGCAAGTG
    GCTGTGAAGG CTTCAAAGAT GGGCGATGTA AGCCAGTCAT CCGAAGAAAG CCCTGGAGGG
    ACTGTCCTGA TGGACACACC CAGTACACAG GATGTACCAG CGTCACCAAC TGCTTCATTA
    GTCACAGAAG GGACAGAAGG AAGAGGCTGT TGGGATGTGG ATCCCGGGCT CCAGGGTGTG
    GTAACCGACT TGGCAGTCTC TGATGCAGGG GAGAAGGTGG AATATAGAAG TTTCTCCGGT
    TCTTCACAGT CAGAGATTAT ACAAGCAATA CAGAATTTAA CGCGTCTCCT CTACAGCCTT
    CAGGCTGCCT TGACCATCCA GGACAGTCAC ATTGAGATCC ACAAACTGGT TCTACAGCAG
    CGGGAGAGCT TGGCCCCTAG CCACTCCTTC CGAGGTGGCC CCTTGCAAGA CCAGGAGAAA
    TCCCGCTACC TGGAAAAGCA GCGTGAAGAG CTGGCCAACA TCCACAAGCT TCAACACCAG
    TTCCAGCAGG AGCAGCGGCG CTGGCACCGC ACGTGCGACC AGCAACAGCG CGAGCAGGAA
    GCCCAAGAGA GCTGGCTGCA GGCGCGGGAA CGTGAGTGCC AGTCCCAGGA GGAATTGTTG
    CTGCGCCACC GTAGCGAGCT GGACCACCAA CTCCAGGAGT ACCAGCAGAG CCTTGAGCGG
    CTACGGGAGG GCCAGCGGAT GGTGGAGAGG GAGAGACAGA AGATGCGTGT CCAGCAGGGT
    CTGCTGGGCC ACTGCAAGCA TAGTCGCCAG CGGAGCCTCC CAGCCGTCTT CTCTCCAGGG
    AGCAAGGAGG TAACAGAACT TAACCGAGCT GAGAGTTTGT GTCATGAAAA TTCATTTTTC
    ATCAATGAAG CTTTTGGACA CATGTCACTT AACACGTCCA ATAAACCAAA CCCATCAGGG
    GTCCCCTGGG ATGCTCACCC CCTGGAGGGG TCTCATTTTG ATTTGGCAAG GACTAGTGAG
    AGTCCAACAG AACTGAAGAT AGACATTTCT CAGCCTCCTG ATGTCAACTC TGAACTCTGG
    ACAACGGGGC CGGGCCATCA GAGACCTGCT CTCCAAGAAA ACAGCAAGGA GTCTTATAAA
    AATGTTGCAG ATTTGGACTC CTTCCAGAGT GAATCGTCAT CTCCCCAGGA CTCCAATCAG
    CGTGGCCCCC AGCCACAGAC ACTCACAACA GAAGCAAAAC TAAGTCTTCC GATGGCGGCT
    GGACACGGTG GCGATGCTGG AGACGGGGCT GAAGAAAACA TTCTTTACCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu78409
    Clone ID Related Accession (Same CDS sequence) XM_017315361.1 , XM_017315361.2
    Accession Version XM_017315361.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4854bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 28 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315361.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGCTCTG GCTCAGTGAC CTACGTGGAC AACATGGCCT GCAGGCTGGC TCGTCTGCTG 
    GTCACTCAGG CTGACCGACT CACGGCCTGC AGTCACCAGA CGCTGCTGAC CCCGTTTGCC
    CTGACAGTGG AAGCGCTGCC TGCCTTGGAT GAGGAGCTCG TGCTGGCTCT GACCCAACTG
    GAGCTGCCTT TGGGTTGGAC AGTCCTGGGA AATTCCTCAC TTGAAGTATC TCTACACAGA
    GAGTCTCTCC TGCATCTGGC TGTGAGATGG GCCCTCCCTA AGCTATTCCA CTTCCTCTTG
    TGTCTCCCTG GAGGAGTCAA GGCCTTGAAG CTACCTAATG AAGAGGCCAC CACACCATTA
    GACTTAGCTT TGCAAGGTGG ACACTCCACA CTGGTAGAAG ACATCACAAA TTTTCAGGGT
    AGCCATTCCC CAGGCTTCTC CCGACTGCGT CTTAACGAAG AGGCCACTCT GCAGTTTGTT
    CATTCATCGG AAACATTGAC CCTGACCGTT AACCACACAG CTGAGCATCT CTTGGAAGCA
    GACATCAAAC TCTTCCGGAA ATATTTCTGG GACAGAGCCT TTCTTGTCAA GGCTCTTGAG
    CAAGAAGCCA AGACTGAGAA AGCAACCATG CCTTCTGGAG CTGCAGAAAC TGAAGAAGAG
    GTTAGGAATT TGGAGTCTGG CAGATCACCA TCGGAGGAGG AGGAGGATGC AAAGAGCATC
    AAAAGCCAGG TGGATGGGCC GAGTGAACAC GAAGACCAGG ACCGCCTACC TCTGGATCGC
    TCCTTTGATG GCCTCAAAAA ATCCAAGCAT GTCCCAGCGT CCCTTGCTGC AGGCCAGCTC
    TCTGATGTTC TGAACGGGGG TGATGAAGTC TACGCTAACT GTATGGTGAT TGACCAGGTT
    GGCGATTTGG ATATCAACTA TATAAACCTA GAGGGACTCT CCACCCATAC GAGTCCCGAG
    TCTGGGAGGT CCATGCTGGG GCCTCAGGCT TGCATGCACA CCCTTCCTCC CGACACCAGT
    CCCTGCGGGC GCCCACTCAT TGAGAACAGT GAGGGGACCC TGGATGCTGC AGCTTCGCAG
    TCTTTCGTGA CACCATCCAG TTCTCGCACT TCCAACTTGA ACCTTTCTTT TGGTCTCCAT
    GGATTTGAAA AGGAGCAGAG TCATCTCAAG AAAAGGAGTT CTAGCCTGGA TGCCCTGGTT
    GCCGACAGTG AGGGGGAAGG AGGTTCTGAG CCCCCCATCT GCTATGCTGT GGGCTCTCAG
    AGTTCCCCAA GAACTGGACT CCCCAGCGGG GATGAACTGG ACTCTTTTGA GACCAACACT
    GAACCAGACT GTAATATCTC CAGGACCGAA TCACTCTCTC TGTCCAGTAC TCTGCATTCA
    AAGGAGTCCT TGCTTTCTGG GATCCGGTCT CGCTCCTACT CCTGCTCATC ACCCAAAATC
    TCTTCAGGAA AATCCCGGCT AGTGCGCGAC TTCACAGTGT GCAGTACCAG CGAAGAACAG
    AGATCATATA GTTTCCAGGA GCCACCAGGA GAAAAAAGGA TTCAGGAAGA AGAATGGGAT
    GAATACGTCA TTCCTGCCAA ATCCGAGTCT GAAAAGTACA AAGTGAGCCG AACTTTCAGC
    TTCCTCATGA ACAGGATGAC CAGCCCACGG AACAAATCTA AGATGAAAAA CAAGGACACT
    AAAGAAAAAG AAAAAATGAA TCGACATCAG TTTGTCCCGG GAACGTTCTC CGGGGTTCTG
    CAGTGCTCCG GTTGTGATAA AACACTCCTG GGGAAAGAGT CACTCCAGTG TGCCAACTGT
    AAGGCAAACA CACACAAGGG CTGCAAAGAT GCTGTGCCTC CGTGTACCAA GAAATTCCAA
    GAGAAATACA ACAAGAACAA ACCACAGAGC ATCCTTGGAA GCTCTTCAGT CAGAGACGTC
    CCAGCACCTG GGCTCTCCTT GCACCCTTCT TCCTCTATGC CCATCGGACT GCCAGCTGGA
    AGGAAGGAAT TTGCGGCTCA GGTGCATCCA CTGTCCAGAA GTGTCCCCGG CACCACTTTG
    GAAAGCTTCA GGAGGGCAGT GACATCGTTA GAGTCTGAGG GTGACAGCTG GAGAAGCAGG
    TCTCATTCCG ATGAGCTGTT CCAGTCTATG GGCTCTTCTC CTTCCACGGA GTCATTCATG
    ATGGAAGATG TTGTGGATTC TTCTCTGTGG ATCGACCTCA GTAGTGATGC TCAGGAGTTT
    GAAGCAGAGT CTTGGAGTCT CGTGGTGGAC CCTTCCTTTT GCAGTAGGCA GGAGAAGGAT
    GTCATCAAAA GACAAGATGT CATTTTTGAG CTGATGCAGA CAGAGGTCCA CCACATCCAG
    ACTCTGCTCA TCATGTCCGA GGTCTTCAGG AAAGGCATGA AGGAGGAACT GCAGCTGGAC
    CACAGCACGG TGGACAAGAT CTTCCCCTGC TTAGATGAGC TGCTGGAGAC TCACCGGCAC
    TTCTTCTTCA GCATGAAGGA GCGCAGGCAG GAGTCCTGCG CTGGCAGTGA CAGGAACTTT
    GTGATCAACC AAATTGGAGA CATTCTGGTA CAACAGTTTT CAGAGGAAAA TGCAAGCAAA
    ATGAAGAGGA TCTATGGAGA ATTCTGCAGC CATCACAAAG AAGCCATGAG TCTCTTTAAA
    GAGCTGCAGC AGAACAAGAA GTTTCAGAAT TTTATTAAGA TCCGAAATAG CAACCTGTTG
    GCTCGTCGCC GCGGAATCCC GGAGTGCATC CTGCTAGTTA CCCAGCGCAT CACAAAGTAC
    CCAGTCTTGG TGGAAAGGAT CCTGCAGTAC ACCAAGGAAA GGACTGAAGA GCACCGGGAC
    CTGTGCAAAG CTCTCGGCTT AATTAAAGAC ATGATCGCAG CCGTGGACCT GAAAGTCAGC
    GAATACGAGA AGAACCAAAA ATGGCTCGAG ATCCTAAATA AAATCGAGAA CAAAACATAC
    ACAAAGCTCA AAAATGGCCA CGTGTTTAGA AAGCAGGCGC TGCTGAGCCA GGAACGGGCT
    CTCCTGCATG ATGGGCTTGT CTACTGGAAA ACCGCTACGG GTCGCTTCAA AGACATCCTG
    GCTCTGCTAC TAACTGACGT GCTGCTCTTC TTACAAGAAA AAGACCAGAA ATACATCTTT
    GCAGCTGTTG ACCAGAAGCC ATCAGTGATT TCCCTCCAGA AGCTGATTGC TAGAGAAGTT
    GCCAACGAGG AGAGAGGCAT GTTTCTGATC AGTGCTTCGT CAGCCGGCCC CGAGATGTAC
    GAAATCCACA CCAATTCCAA GGAGGAGCGG AATAACTGGA TGAGGCGGAT CCAGCAGGCA
    GTAGAAAGTT GTCCAGAGGA AGAAGGAGGA AGGACGAGCG AGTCGGATGA AGAGAGGCGG
    AAAGCTGAAG CTAGAGTGGC CAAGATTCAG CAATGTCAAG AAATACTCAG TAACCAAGAC
    CAACAGATTT GCACGTACTT GGAAGAAAAG CTGCATATCT ACGCTGAACT TGGGGAACTG
    AGTGGCTTTG AGGATGTCCA CCTAGAACCC CATCTGCTCA TCAAACCTGA CCCTGGAGAG
    CCTCCCCAGG CAGCGTCCCT GCTTGCAGCA GCCCTGAGAG AAGCCGAGAG TCTGCAAGTG
    GCTGTGAAGG CTTCAAAGAT GGGCGATGTA AGCCAGTCAT CCGAAGAAAG CCCTGGAGGG
    ACTGTCCTGA TGGACACACC CAGTACACAG GATGTACCAG CGTCACCAAC TGCTTCATTA
    GTCACAGAAG GGACAGAAGG AAGAGGCTGT TGGGATGTGG ATCCCGGGCT CCAGGGTGTG
    GTAACCGACT TGGCAGTCTC TGATGCAGGG GAGAAGGTGG AATATAGAAG TTTCTCCGGT
    TCTTCACAGT CAGAGATTAT ACAAGCAATA CAGAATTTAA CGCGTCTCCT CTACAGCCTT
    CAGGCTGCCT TGACCATCCA GGACAGTCAC ATTGAGATCC ACAAACTGGT TCTACAGCAG
    CGGGAGAGCT TGGCCCCTAG CCACTCCTTC CGAGGTGGCC CCTTGCAAGA CCAGGAGAAA
    TCCCGCTACC TGGAAAAGCA GCGTGAAGAG CTGGCCAACA TCCACAAGCT TCAACACCAG
    TTCCAGCAGG AGCAGCGGCG CTGGCACCGC ACGTGCGACC AGCAACAGCG CGAGCAGGAA
    GCCCAAGAGA GCTGGCTGCA GGCGCGGGAA CGTGAGTGCC AGTCCCAGGA GGAATTGTTG
    CTGCGCCACC GTAGCGAGCT GGACCACCAA CTCCAGGAGT ACCAGCAGAG CCTTGAGCGG
    CTACGGGAGG GCCAGCGGAT GGTGGAGAGG GAGAGACAGA AGATGCGTGT CCAGCAGGGT
    CTGCTGGGCC ACTGCAAGCA TAGTCGCCAG CGGAGCCTCC CAGCCGTCTT CTCTCCAGGG
    AGCAAGGAGG TAACAGAACT TAACCGAGCT GAGAGTTTGT GTCATGAAAA TTCATTTTTC
    ATCAATGAAG CTTTTGGACA CATGTCACTT AACACGTCCA ATAAACCAAA CCCATCAGGG
    GTCCCCTGGG ATGCTCACCC CCTGGAGGGG TCTCATTTTG ATTTGGCAAG GACTAGTGAG
    AGTCCAACAG AACTGAAGAT AGACATTTCT CAGCCTCCTG ATGTCAACTC TGAACTCTGG
    ACAACGGGGC CGGGCCATCA GAGACCTGCT CTCCAAGAAA ACAGCAAGGA GTCTTATAAA
    AATGTTGCAG ATTTGGACTC CTTCCAGAGT GAATCGTCAT CTCCCCAGGA CTCCAATCAG
    CGTGGCCCCC AGCCACAGAC ACTCACAACA GAAGCAAAAC TAAGTCTTCC GATGGCGGCT
    GGACACGGTG GCGATGCTGG AGACGGGGCT GAAGAAAACA TTCTTTACCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170850.1
    CDS235..5088
    Translation

    Target ORF information:

    RefSeq Version XM_017315361.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017315361.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGCTCTG GCTCAGTGAC CTACGTGGAC AACATGGCCT GCAGGCTGGC TCGTCTGCTG 
    GTCACTCAGG CTGACCGACT CACGGCCTGC AGTCACCAGA CGCTGCTGAC CCCGTTTGCC
    CTGACAGTGG AAGCGCTGCC TGCCTTGGAT GAGGAGCTCG TGCTGGCTCT GACCCAACTG
    GAGCTGCCTT TGGGTTGGAC AGTCCTGGGA AATTCCTCAC TTGAAGTATC TCTACACAGA
    GAGTCTCTCC TGCATCTGGC TGTGAGATGG GCCCTCCCTA AGCTATTCCA CTTCCTCTTG
    TGTCTCCCTG GAGGAGTCAA GGCCTTGAAG CTACCTAATG AAGAGGCCAC CACACCATTA
    GACTTAGCTT TGCAAGGTGG ACACTCCACA CTGGTAGAAG ACATCACAAA TTTTCAGGGT
    AGCCATTCCC CAGGCTTCTC CCGACTGCGT CTTAACGAAG AGGCCACTCT GCAGTTTGTT
    CATTCATCGG AAACATTGAC CCTGACCGTT AACCACACAG CTGAGCATCT CTTGGAAGCA
    GACATCAAAC TCTTCCGGAA ATATTTCTGG GACAGAGCCT TTCTTGTCAA GGCTCTTGAG
    CAAGAAGCCA AGACTGAGAA AGCAACCATG CCTTCTGGAG CTGCAGAAAC TGAAGAAGAG
    GTTAGGAATT TGGAGTCTGG CAGATCACCA TCGGAGGAGG AGGAGGATGC AAAGAGCATC
    AAAAGCCAGG TGGATGGGCC GAGTGAACAC GAAGACCAGG ACCGCCTACC TCTGGATCGC
    TCCTTTGATG GCCTCAAAAA ATCCAAGCAT GTCCCAGCGT CCCTTGCTGC AGGCCAGCTC
    TCTGATGTTC TGAACGGGGG TGATGAAGTC TACGCTAACT GTATGGTGAT TGACCAGGTT
    GGCGATTTGG ATATCAACTA TATAAACCTA GAGGGACTCT CCACCCATAC GAGTCCCGAG
    TCTGGGAGGT CCATGCTGGG GCCTCAGGCT TGCATGCACA CCCTTCCTCC CGACACCAGT
    CCCTGCGGGC GCCCACTCAT TGAGAACAGT GAGGGGACCC TGGATGCTGC AGCTTCGCAG
    TCTTTCGTGA CACCATCCAG TTCTCGCACT TCCAACTTGA ACCTTTCTTT TGGTCTCCAT
    GGATTTGAAA AGGAGCAGAG TCATCTCAAG AAAAGGAGTT CTAGCCTGGA TGCCCTGGTT
    GCCGACAGTG AGGGGGAAGG AGGTTCTGAG CCCCCCATCT GCTATGCTGT GGGCTCTCAG
    AGTTCCCCAA GAACTGGACT CCCCAGCGGG GATGAACTGG ACTCTTTTGA GACCAACACT
    GAACCAGACT GTAATATCTC CAGGACCGAA TCACTCTCTC TGTCCAGTAC TCTGCATTCA
    AAGGAGTCCT TGCTTTCTGG GATCCGGTCT CGCTCCTACT CCTGCTCATC ACCCAAAATC
    TCTTCAGGAA AATCCCGGCT AGTGCGCGAC TTCACAGTGT GCAGTACCAG CGAAGAACAG
    AGATCATATA GTTTCCAGGA GCCACCAGGA GAAAAAAGGA TTCAGGAAGA AGAATGGGAT
    GAATACGTCA TTCCTGCCAA ATCCGAGTCT GAAAAGTACA AAGTGAGCCG AACTTTCAGC
    TTCCTCATGA ACAGGATGAC CAGCCCACGG AACAAATCTA AGATGAAAAA CAAGGACACT
    AAAGAAAAAG AAAAAATGAA TCGACATCAG TTTGTCCCGG GAACGTTCTC CGGGGTTCTG
    CAGTGCTCCG GTTGTGATAA AACACTCCTG GGGAAAGAGT CACTCCAGTG TGCCAACTGT
    AAGGCAAACA CACACAAGGG CTGCAAAGAT GCTGTGCCTC CGTGTACCAA GAAATTCCAA
    GAGAAATACA ACAAGAACAA ACCACAGAGC ATCCTTGGAA GCTCTTCAGT CAGAGACGTC
    CCAGCACCTG GGCTCTCCTT GCACCCTTCT TCCTCTATGC CCATCGGACT GCCAGCTGGA
    AGGAAGGAAT TTGCGGCTCA GGTGCATCCA CTGTCCAGAA GTGTCCCCGG CACCACTTTG
    GAAAGCTTCA GGAGGGCAGT GACATCGTTA GAGTCTGAGG GTGACAGCTG GAGAAGCAGG
    TCTCATTCCG ATGAGCTGTT CCAGTCTATG GGCTCTTCTC CTTCCACGGA GTCATTCATG
    ATGGAAGATG TTGTGGATTC TTCTCTGTGG ATCGACCTCA GTAGTGATGC TCAGGAGTTT
    GAAGCAGAGT CTTGGAGTCT CGTGGTGGAC CCTTCCTTTT GCAGTAGGCA GGAGAAGGAT
    GTCATCAAAA GACAAGATGT CATTTTTGAG CTGATGCAGA CAGAGGTCCA CCACATCCAG
    ACTCTGCTCA TCATGTCCGA GGTCTTCAGG AAAGGCATGA AGGAGGAACT GCAGCTGGAC
    CACAGCACGG TGGACAAGAT CTTCCCCTGC TTAGATGAGC TGCTGGAGAC TCACCGGCAC
    TTCTTCTTCA GCATGAAGGA GCGCAGGCAG GAGTCCTGCG CTGGCAGTGA CAGGAACTTT
    GTGATCAACC AAATTGGAGA CATTCTGGTA CAACAGTTTT CAGAGGAAAA TGCAAGCAAA
    ATGAAGAGGA TCTATGGAGA ATTCTGCAGC CATCACAAAG AAGCCATGAG TCTCTTTAAA
    GAGCTGCAGC AGAACAAGAA GTTTCAGAAT TTTATTAAGA TCCGAAATAG CAACCTGTTG
    GCTCGTCGCC GCGGAATCCC GGAGTGCATC CTGCTAGTTA CCCAGCGCAT CACAAAGTAC
    CCAGTCTTGG TGGAAAGGAT CCTGCAGTAC ACCAAGGAAA GGACTGAAGA GCACCGGGAC
    CTGTGCAAAG CTCTCGGCTT AATTAAAGAC ATGATCGCAG CCGTGGACCT GAAAGTCAGC
    GAATACGAGA AGAACCAAAA ATGGCTCGAG ATCCTAAATA AAATCGAGAA CAAAACATAC
    ACAAAGCTCA AAAATGGCCA CGTGTTTAGA AAGCAGGCGC TGCTGAGCCA GGAACGGGCT
    CTCCTGCATG ATGGGCTTGT CTACTGGAAA ACCGCTACGG GTCGCTTCAA AGACATCCTG
    GCTCTGCTAC TAACTGACGT GCTGCTCTTC TTACAAGAAA AAGACCAGAA ATACATCTTT
    GCAGCTGTTG ACCAGAAGCC ATCAGTGATT TCCCTCCAGA AGCTGATTGC TAGAGAAGTT
    GCCAACGAGG AGAGAGGCAT GTTTCTGATC AGTGCTTCGT CAGCCGGCCC CGAGATGTAC
    GAAATCCACA CCAATTCCAA GGAGGAGCGG AATAACTGGA TGAGGCGGAT CCAGCAGGCA
    GTAGAAAGTT GTCCAGAGGA AGAAGGAGGA AGGACGAGCG AGTCGGATGA AGAGAGGCGG
    AAAGCTGAAG CTAGAGTGGC CAAGATTCAG CAATGTCAAG AAATACTCAG TAACCAAGAC
    CAACAGATTT GCACGTACTT GGAAGAAAAG CTGCATATCT ACGCTGAACT TGGGGAACTG
    AGTGGCTTTG AGGATGTCCA CCTAGAACCC CATCTGCTCA TCAAACCTGA CCCTGGAGAG
    CCTCCCCAGG CAGCGTCCCT GCTTGCAGCA GCCCTGAGAG AAGCCGAGAG TCTGCAAGTG
    GCTGTGAAGG CTTCAAAGAT GGGCGATGTA AGCCAGTCAT CCGAAGAAAG CCCTGGAGGG
    ACTGTCCTGA TGGACACACC CAGTACACAG GATGTACCAG CGTCACCAAC TGCTTCATTA
    GTCACAGAAG GGACAGAAGG AAGAGGCTGT TGGGATGTGG ATCCCGGGCT CCAGGGTGTG
    GTAACCGACT TGGCAGTCTC TGATGCAGGG GAGAAGGTGG AATATAGAAG TTTCTCCGGT
    TCTTCACAGT CAGAGATTAT ACAAGCAATA CAGAATTTAA CGCGTCTCCT CTACAGCCTT
    CAGGCTGCCT TGACCATCCA GGACAGTCAC ATTGAGATCC ACAAACTGGT TCTACAGCAG
    CGGGAGAGCT TGGCCCCTAG CCACTCCTTC CGAGGTGGCC CCTTGCAAGA CCAGGAGAAA
    TCCCGCTACC TGGAAAAGCA GCGTGAAGAG CTGGCCAACA TCCACAAGCT TCAACACCAG
    TTCCAGCAGG AGCAGCGGCG CTGGCACCGC ACGTGCGACC AGCAACAGCG CGAGCAGGAA
    GCCCAAGAGA GCTGGCTGCA GGCGCGGGAA CGTGAGTGCC AGTCCCAGGA GGAATTGTTG
    CTGCGCCACC GTAGCGAGCT GGACCACCAA CTCCAGGAGT ACCAGCAGAG CCTTGAGCGG
    CTACGGGAGG GCCAGCGGAT GGTGGAGAGG GAGAGACAGA AGATGCGTGT CCAGCAGGGT
    CTGCTGGGCC ACTGCAAGCA TAGTCGCCAG CGGAGCCTCC CAGCCGTCTT CTCTCCAGGG
    AGCAAGGAGG TAACAGAACT TAACCGAGCT GAGAGTTTGT GTCATGAAAA TTCATTTTTC
    ATCAATGAAG CTTTTGGACA CATGTCACTT AACACGTCCA ATAAACCAAA CCCATCAGGG
    GTCCCCTGGG ATGCTCACCC CCTGGAGGGG TCTCATTTTG ATTTGGCAAG GACTAGTGAG
    AGTCCAACAG AACTGAAGAT AGACATTTCT CAGCCTCCTG ATGTCAACTC TGAACTCTGG
    ACAACGGGGC CGGGCCATCA GAGACCTGCT CTCCAAGAAA ACAGCAAGGA GTCTTATAAA
    AATGTTGCAG ATTTGGACTC CTTCCAGAGT GAATCGTCAT CTCCCCAGGA CTCCAATCAG
    CGTGGCCCCC AGCCACAGAC ACTCACAACA GAAGCAAAAC TAAGTCTTCC GATGGCGGCT
    GGACACGGTG GCGATGCTGG AGACGGGGCT GAAGAAAACA TTCTTTACCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu09358
    Clone ID Related Accession (Same CDS sequence) NM_012026.2
    Accession Version NM_012026.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5103bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 28
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY778560.1, BC098331.1, BC075734.1 and AC124397.5. On Jan 20, 2007 this sequence version replaced NM_012026.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U73199.1, BC098331.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGTTGA GCTGCAGTGA AGTGCCTCTT TATGGACAGA AGACGGTCTA TGCAAAGTTT 
    GGCAAAAACG TGTATCTACC CGAAGATGCG GAGTTTTACT TTGTTTATGG CGGGTCGCAT
    CAGAGGCACG TGGTGATTGC TGACCGTGTC CAGGACAATG TCCTCCAATC TAGCATTCCA
    GGCCACTGGC TTCAGGAGAC GGTGACGGTA TCTGTGTGCC TCTGCTCGGA AGGGTACTCC
    CCAGTGACCA TGGGCTCTGG CTCAGTGACC TACGTGGACA ACATGGCCTG CAGGCTGGCT
    CGTCTGCTGG TCACTCAGGC TGACCGACTC ACGGCCTGCA GTCACCAGAC GCTGCTGACC
    CCGTTTGCCC TGACAGTGGA AGCGCTGCCT GCCTTGGATG AGGAGCTCGT GCTGGCTCTG
    ACCCAACTGG AGCTGCCTTT GGGTTGGACA GTCCTGGGAA ATTCCTCACT TGAAGTATCT
    CTACACAGAG AGTCTCTCCT GCATCTGGCT GTGAGATGGG CCCTCCCTAA GCTATTCCAC
    TTCCTCTTGT GTCTCCCTGG AGGAGTCAAG GCCTTGAAGC TACCTAATGA AGAGGCCACC
    ACACCATTAG ACTTAGCTTT GCAAGGTGGA CACTCCACAC TGGTAGAAGA CATCACAAAT
    TTTCAGGGTA GCCATTCCCC AGGCTTCTCC CGACTGCGTC TTAACGAAGA GGCCACTCTG
    CAGTTTGTTC ATTCATCGGA AACATTGACC CTGACCGTTA ACCACACAGC TGAGCATCTC
    TTGGAAGCAG ACATCAAACT CTTCCGGAAA TATTTCTGGG ACAGAGCCTT TCTTGTCAAG
    GCTCTTGAGC AAGAAGCCAA GACTGAGAAA GCAACCATGC CTTCTGGAGC TGCAGAAACT
    GAAGAAGAGG TTAGGAATTT GGAGTCTGGC AGATCACCAT CGGAGGAGGA GGAGGATGCA
    AAGAGCATCA AAAGCCAGGT GGATGGGCCG AGTGAACACG AAGACCAGGA CCGCCTACCT
    CTGGATCGCT CCTTTGATGG CCTCAAAAAA TCCAAGCATG TCCCAGCGTC CCTTGCTGCA
    GGCCAGCTCT CTGATGTTCT GAACGGGGGT GATGAAGTCT ACGCTAACTG TATGGTGATT
    GACCAGGTTG GCGATTTGGA TATCAACTAT ATAAACCTAG AGGGACTCTC CACCCATACG
    AGTCCCGAGT CTGGGAGGTC CATGCTGGGG CCTCAGGCTT GCATGCACAC CCTTCCTCCC
    GACACCAGTC CCTGCGGGCG CCCACTCATT GAGAACAGTG AGGGGACCCT GGATGCTGCA
    GCTTCGCAGT CTTTCGTGAC ACCATCCAGT TCTCGCACTT CCAACTTGAA CCTTTCTTTT
    GGTCTCCATG GATTTGAAAA GGAGCAGAGT CATCTCAAGA AAAGGAGTTC TAGCCTGGAT
    GCCCTGGTTG CCGACAGTGA GGGGGAAGGA GGTTCTGAGC CCCCCATCTG CTATGCTGTG
    GGCTCTCAGA GTTCCCCAAG AACTGGACTC CCCAGCGGGG ATGAACTGGA CTCTTTTGAG
    ACCAACACTG AACCAGACTG TAATATCTCC AGGACCGAAT CACTCTCTCT GTCCAGTACT
    CTGCATTCAA AGGAGTCCTT GCTTTCTGGG ATCCGGTCTC GCTCCTACTC CTGCTCATCA
    CCCAAAATCT CTTCAGGAAA ATCCCGGCTA GTGCGCGACT TCACAGTGTG CAGTACCAGC
    GAAGAACAGA GATCATATAG TTTCCAGGAG CCACCAGGAG AAAAAAGGAT TCAGGAAGAA
    GAATGGGATG AATACGTCAT TCCTGCCAAA TCCGAGTCTG AAAAGTACAA AGTGAGCCGA
    ACTTTCAGCT TCCTCATGAA CAGGATGACC AGCCCACGGA ACAAATCTAA GATGAAAAAC
    AAGGACACTA AAGAAAAAGA AAAAATGAAT CGACATCAGT TTGTCCCGGG AACGTTCTCC
    GGGGTTCTGC AGTGCTCCGG TTGTGATAAA ACACTCCTGG GGAAAGAGTC ACTCCAGTGT
    GCCAACTGTA AGGCAAACAC ACACAAGGGC TGCAAAGATG CTGTGCCTCC GTGTACCAAG
    AAATTCCAAG AGAAATACAA CAAGAACAAA CCACAGAGCA TCCTTGGAAG CTCTTCAGTC
    AGAGACGTCC CAGCACCTGG GCTCTCCTTG CACCCTTCTT CCTCTATGCC CATCGGACTG
    CCAGCTGGAA GGAAGGAATT TGCGGCTCAG GTGCATCCAC TGTCCAGAAG TGTCCCCGGC
    ACCACTTTGG AAAGCTTCAG GAGGGCAGTG ACATCGTTAG AGTCTGAGGG TGACAGCTGG
    AGAAGCAGGT CTCATTCCGA TGAGCTGTTC CAGTCTATGG GCTCTTCTCC TTCCACGGAG
    TCATTCATGA TGGAAGATGT TGTGGATTCT TCTCTGTGGA TCGACCTCAG TAGTGATGCT
    CAGGAGTTTG AAGCAGAGTC TTGGAGTCTC GTGGTGGACC CTTCCTTTTG CAGTAGGCAG
    GAGAAGGATG TCATCAAAAG ACAAGATGTC ATTTTTGAGC TGATGCAGAC AGAGGTCCAC
    CACATCCAGA CTCTGCTCAT CATGTCCGAG GTCTTCAGGA AAGGCATGAA GGAGGAACTG
    CAGCTGGACC ACAGCACGGT GGACAAGATC TTCCCCTGCT TAGATGAGCT GCTGGAGACT
    CACCGGCACT TCTTCTTCAG CATGAAGGAG CGCAGGCAGG AGTCCTGCGC TGGCAGTGAC
    AGGAACTTTG TGATCAACCA AATTGGAGAC ATTCTGGTAC AACAGTTTTC AGAGGAAAAT
    GCAAGCAAAA TGAAGAGGAT CTATGGAGAA TTCTGCAGCC ATCACAAAGA AGCCATGAGT
    CTCTTTAAAG AGCTGCAGCA GAACAAGAAG TTTCAGAATT TTATTAAGAT CCGAAATAGC
    AACCTGTTGG CTCGTCGCCG CGGAATCCCG GAGTGCATCC TGCTAGTTAC CCAGCGCATC
    ACAAAGTACC CAGTCTTGGT GGAAAGGATC CTGCAGTACA CCAAGGAAAG GACTGAAGAG
    CACCGGGACC TGTGCAAAGC TCTCGGCTTA ATTAAAGACA TGATCGCAGC CGTGGACCTG
    AAAGTCAGCG AATACGAGAA GAACCAAAAA TGGCTCGAGA TCCTAAATAA AATCGAGAAC
    AAAACATACA CAAAGCTCAA AAATGGCCAC GTGTTTAGAA AGCAGGCGCT GCTGAGCCAG
    GAACGGGCTC TCCTGCATGA TGGGCTTGTC TACTGGAAAA CCGCTACGGG TCGCTTCAAA
    GACATCCTGG CTCTGCTACT AACTGACGTG CTGCTCTTCT TACAAGAAAA AGACCAGAAA
    TACATCTTTG CAGCTGTTGA CCAGAAGCCA TCAGTGATTT CCCTCCAGAA GCTGATTGCT
    AGAGAAGTTG CCAACGAGGA GAGAGGCATG TTTCTGATCA GTGCTTCGTC AGCCGGCCCC
    GAGATGTACG AAATCCACAC CAATTCCAAG GAGGAGCGGA ATAACTGGAT GAGGCGGATC
    CAGCAGGCAG TAGAAAGTTG TCCAGAGGAA GAAGGAGGAA GGACGAGCGA GTCGGATGAA
    GAGAGGCGGA AAGCTGAAGC TAGAGTGGCC AAGATTCAGC AATGTCAAGA AATACTCAGT
    AACCAAGACC AACAGATTTG CACGTACTTG GAAGAAAAGC TGCATATCTA CGCTGAACTT
    GGGGAACTGA GTGGCTTTGA GGATGTCCAC CTAGAACCCC ATCTGCTCAT CAAACCTGAC
    CCTGGAGAGC CTCCCCAGGC AGCGTCCCTG CTTGCAGCAG CCCTGAGAGA AGCCGAGAGT
    CTGCAAGTGG CTGTGAAGGC TTCAAAGATG GGCGATGTAA GCCAGTCATC CGAAGAAAGC
    CCTGGAGGGA CTGTCCTGAT GGACACACCC AGTACACAGG ATGTACCAGC GTCACCAACT
    GCTTCATTAG TCACAGAAGG GACAGAAGGA AGAGGCTGTT GGGATGTGGA TCCCGGGCTC
    CAGGGTGTGG TAACCGACTT GGCAGTCTCT GATGCAGGGG AGAAGGTGGA ATATAGAAGT
    TTCTCCGGTT CTTCACAGTC AGAGATTATA CAAGCAATAC AGAATTTAAC GCGTCTCCTC
    TACAGCCTTC AGGCTGCCTT GACCATCCAG GACAGTCACA TTGAGATCCA CAAACTGGTT
    CTACAGCAGC GGGAGAGCTT GGCCCCTAGC CACTCCTTCC GAGGTGGCCC CTTGCAAGAC
    CAGGAGAAAT CCCGCTACCT GGAAAAGCAG CGTGAAGAGC TGGCCAACAT CCACAAGCTT
    CAACACCAGT TCCAGCAGGA GCAGCGGCGC TGGCACCGCA CGTGCGACCA GCAACAGCGC
    GAGCAGGAAG CCCAAGAGAG CTGGCTGCAG GCGCGGGAAC GTGAGTGCCA GTCCCAGGAG
    GAATTGTTGC TGCGCCACCG TAGCGAGCTG GACCACCAAC TCCAGGAGTA CCAGCAGAGC
    CTTGAGCGGC TACGGGAGGG CCAGCGGATG GTGGAGAGGG AGAGACAGAA GATGCGTGTC
    CAGCAGGGTC TGCTGGGCCA CTGCAAGCAT AGTCGCCAGC GGAGCCTCCC AGCCGTCTTC
    TCTCCAGGGA GCAAGGAGGT AACAGAACTT AACCGAGCTG AGAGTTTGTG TCATGAAAAT
    TCATTTTTCA TCAATGAAGC TTTTGGACAC ATGTCACTTA ACACGTCCAA TAAACCAAAC
    CCATCAGGGG TCCCCTGGGA TGCTCACCCC CTGGAGGGGT CTCATTTTGA TTTGGCAAGG
    ACTAGTGAGA GTCCAACAGA ACTGAAGATA GACATTTCTC AGCCTCCTGA TGTCAACTCT
    GAACTCTGGA CAACGGGGCC GGGCCATCAG AGACCTGCTC TCCAAGAAAA CAGCAAGGAG
    TCTTATAAAA ATGTTGCAGA TTTGGACTCC TTCCAGAGTG AATCGTCATC TCCCCAGGAC
    TCCAATCAGC GTGGCCCCCA GCCACAGACA CTCACAACAG AAGCAAAACT AAGTCTTCCG
    ATGGCGGCTG GACACGGTGG CGATGCTGGA GACGGGGCTG AAGAAAACAT TCTTTACCTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_036156.2
    CDS105..5207
    Translation

    Target ORF information:

    RefSeq Version NM_012026.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_012026.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGTTGA GCTGCAGTGA AGTGCCTCTT TATGGACAGA AGACGGTCTA TGCAAAGTTT 
    GGCAAAAACG TGTATCTACC CGAAGATGCG GAGTTTTACT TTGTTTATGG CGGGTCGCAT
    CAGAGGCACG TGGTGATTGC TGACCGTGTC CAGGACAATG TCCTCCAATC TAGCATTCCA
    GGCCACTGGC TTCAGGAGAC GGTGACGGTA TCTGTGTGCC TCTGCTCGGA AGGGTACTCC
    CCAGTGACCA TGGGCTCTGG CTCAGTGACC TACGTGGACA ACATGGCCTG CAGGCTGGCT
    CGTCTGCTGG TCACTCAGGC TGACCGACTC ACGGCCTGCA GTCACCAGAC GCTGCTGACC
    CCGTTTGCCC TGACAGTGGA AGCGCTGCCT GCCTTGGATG AGGAGCTCGT GCTGGCTCTG
    ACCCAACTGG AGCTGCCTTT GGGTTGGACA GTCCTGGGAA ATTCCTCACT TGAAGTATCT
    CTACACAGAG AGTCTCTCCT GCATCTGGCT GTGAGATGGG CCCTCCCTAA GCTATTCCAC
    TTCCTCTTGT GTCTCCCTGG AGGAGTCAAG GCCTTGAAGC TACCTAATGA AGAGGCCACC
    ACACCATTAG ACTTAGCTTT GCAAGGTGGA CACTCCACAC TGGTAGAAGA CATCACAAAT
    TTTCAGGGTA GCCATTCCCC AGGCTTCTCC CGACTGCGTC TTAACGAAGA GGCCACTCTG
    CAGTTTGTTC ATTCATCGGA AACATTGACC CTGACCGTTA ACCACACAGC TGAGCATCTC
    TTGGAAGCAG ACATCAAACT CTTCCGGAAA TATTTCTGGG ACAGAGCCTT TCTTGTCAAG
    GCTCTTGAGC AAGAAGCCAA GACTGAGAAA GCAACCATGC CTTCTGGAGC TGCAGAAACT
    GAAGAAGAGG TTAGGAATTT GGAGTCTGGC AGATCACCAT CGGAGGAGGA GGAGGATGCA
    AAGAGCATCA AAAGCCAGGT GGATGGGCCG AGTGAACACG AAGACCAGGA CCGCCTACCT
    CTGGATCGCT CCTTTGATGG CCTCAAAAAA TCCAAGCATG TCCCAGCGTC CCTTGCTGCA
    GGCCAGCTCT CTGATGTTCT GAACGGGGGT GATGAAGTCT ACGCTAACTG TATGGTGATT
    GACCAGGTTG GCGATTTGGA TATCAACTAT ATAAACCTAG AGGGACTCTC CACCCATACG
    AGTCCCGAGT CTGGGAGGTC CATGCTGGGG CCTCAGGCTT GCATGCACAC CCTTCCTCCC
    GACACCAGTC CCTGCGGGCG CCCACTCATT GAGAACAGTG AGGGGACCCT GGATGCTGCA
    GCTTCGCAGT CTTTCGTGAC ACCATCCAGT TCTCGCACTT CCAACTTGAA CCTTTCTTTT
    GGTCTCCATG GATTTGAAAA GGAGCAGAGT CATCTCAAGA AAAGGAGTTC TAGCCTGGAT
    GCCCTGGTTG CCGACAGTGA GGGGGAAGGA GGTTCTGAGC CCCCCATCTG CTATGCTGTG
    GGCTCTCAGA GTTCCCCAAG AACTGGACTC CCCAGCGGGG ATGAACTGGA CTCTTTTGAG
    ACCAACACTG AACCAGACTG TAATATCTCC AGGACCGAAT CACTCTCTCT GTCCAGTACT
    CTGCATTCAA AGGAGTCCTT GCTTTCTGGG ATCCGGTCTC GCTCCTACTC CTGCTCATCA
    CCCAAAATCT CTTCAGGAAA ATCCCGGCTA GTGCGCGACT TCACAGTGTG CAGTACCAGC
    GAAGAACAGA GATCATATAG TTTCCAGGAG CCACCAGGAG AAAAAAGGAT TCAGGAAGAA
    GAATGGGATG AATACGTCAT TCCTGCCAAA TCCGAGTCTG AAAAGTACAA AGTGAGCCGA
    ACTTTCAGCT TCCTCATGAA CAGGATGACC AGCCCACGGA ACAAATCTAA GATGAAAAAC
    AAGGACACTA AAGAAAAAGA AAAAATGAAT CGACATCAGT TTGTCCCGGG AACGTTCTCC
    GGGGTTCTGC AGTGCTCCGG TTGTGATAAA ACACTCCTGG GGAAAGAGTC ACTCCAGTGT
    GCCAACTGTA AGGCAAACAC ACACAAGGGC TGCAAAGATG CTGTGCCTCC GTGTACCAAG
    AAATTCCAAG AGAAATACAA CAAGAACAAA CCACAGAGCA TCCTTGGAAG CTCTTCAGTC
    AGAGACGTCC CAGCACCTGG GCTCTCCTTG CACCCTTCTT CCTCTATGCC CATCGGACTG
    CCAGCTGGAA GGAAGGAATT TGCGGCTCAG GTGCATCCAC TGTCCAGAAG TGTCCCCGGC
    ACCACTTTGG AAAGCTTCAG GAGGGCAGTG ACATCGTTAG AGTCTGAGGG TGACAGCTGG
    AGAAGCAGGT CTCATTCCGA TGAGCTGTTC CAGTCTATGG GCTCTTCTCC TTCCACGGAG
    TCATTCATGA TGGAAGATGT TGTGGATTCT TCTCTGTGGA TCGACCTCAG TAGTGATGCT
    CAGGAGTTTG AAGCAGAGTC TTGGAGTCTC GTGGTGGACC CTTCCTTTTG CAGTAGGCAG
    GAGAAGGATG TCATCAAAAG ACAAGATGTC ATTTTTGAGC TGATGCAGAC AGAGGTCCAC
    CACATCCAGA CTCTGCTCAT CATGTCCGAG GTCTTCAGGA AAGGCATGAA GGAGGAACTG
    CAGCTGGACC ACAGCACGGT GGACAAGATC TTCCCCTGCT TAGATGAGCT GCTGGAGACT
    CACCGGCACT TCTTCTTCAG CATGAAGGAG CGCAGGCAGG AGTCCTGCGC TGGCAGTGAC
    AGGAACTTTG TGATCAACCA AATTGGAGAC ATTCTGGTAC AACAGTTTTC AGAGGAAAAT
    GCAAGCAAAA TGAAGAGGAT CTATGGAGAA TTCTGCAGCC ATCACAAAGA AGCCATGAGT
    CTCTTTAAAG AGCTGCAGCA GAACAAGAAG TTTCAGAATT TTATTAAGAT CCGAAATAGC
    AACCTGTTGG CTCGTCGCCG CGGAATCCCG GAGTGCATCC TGCTAGTTAC CCAGCGCATC
    ACAAAGTACC CAGTCTTGGT GGAAAGGATC CTGCAGTACA CCAAGGAAAG GACTGAAGAG
    CACCGGGACC TGTGCAAAGC TCTCGGCTTA ATTAAAGACA TGATCGCAGC CGTGGACCTG
    AAAGTCAGCG AATACGAGAA GAACCAAAAA TGGCTCGAGA TCCTAAATAA AATCGAGAAC
    AAAACATACA CAAAGCTCAA AAATGGCCAC GTGTTTAGAA AGCAGGCGCT GCTGAGCCAG
    GAACGGGCTC TCCTGCATGA TGGGCTTGTC TACTGGAAAA CCGCTACGGG TCGCTTCAAA
    GACATCCTGG CTCTGCTACT AACTGACGTG CTGCTCTTCT TACAAGAAAA AGACCAGAAA
    TACATCTTTG CAGCTGTTGA CCAGAAGCCA TCAGTGATTT CCCTCCAGAA GCTGATTGCT
    AGAGAAGTTG CCAACGAGGA GAGAGGCATG TTTCTGATCA GTGCTTCGTC AGCCGGCCCC
    GAGATGTACG AAATCCACAC CAATTCCAAG GAGGAGCGGA ATAACTGGAT GAGGCGGATC
    CAGCAGGCAG TAGAAAGTTG TCCAGAGGAA GAAGGAGGAA GGACGAGCGA GTCGGATGAA
    GAGAGGCGGA AAGCTGAAGC TAGAGTGGCC AAGATTCAGC AATGTCAAGA AATACTCAGT
    AACCAAGACC AACAGATTTG CACGTACTTG GAAGAAAAGC TGCATATCTA CGCTGAACTT
    GGGGAACTGA GTGGCTTTGA GGATGTCCAC CTAGAACCCC ATCTGCTCAT CAAACCTGAC
    CCTGGAGAGC CTCCCCAGGC AGCGTCCCTG CTTGCAGCAG CCCTGAGAGA AGCCGAGAGT
    CTGCAAGTGG CTGTGAAGGC TTCAAAGATG GGCGATGTAA GCCAGTCATC CGAAGAAAGC
    CCTGGAGGGA CTGTCCTGAT GGACACACCC AGTACACAGG ATGTACCAGC GTCACCAACT
    GCTTCATTAG TCACAGAAGG GACAGAAGGA AGAGGCTGTT GGGATGTGGA TCCCGGGCTC
    CAGGGTGTGG TAACCGACTT GGCAGTCTCT GATGCAGGGG AGAAGGTGGA ATATAGAAGT
    TTCTCCGGTT CTTCACAGTC AGAGATTATA CAAGCAATAC AGAATTTAAC GCGTCTCCTC
    TACAGCCTTC AGGCTGCCTT GACCATCCAG GACAGTCACA TTGAGATCCA CAAACTGGTT
    CTACAGCAGC GGGAGAGCTT GGCCCCTAGC CACTCCTTCC GAGGTGGCCC CTTGCAAGAC
    CAGGAGAAAT CCCGCTACCT GGAAAAGCAG CGTGAAGAGC TGGCCAACAT CCACAAGCTT
    CAACACCAGT TCCAGCAGGA GCAGCGGCGC TGGCACCGCA CGTGCGACCA GCAACAGCGC
    GAGCAGGAAG CCCAAGAGAG CTGGCTGCAG GCGCGGGAAC GTGAGTGCCA GTCCCAGGAG
    GAATTGTTGC TGCGCCACCG TAGCGAGCTG GACCACCAAC TCCAGGAGTA CCAGCAGAGC
    CTTGAGCGGC TACGGGAGGG CCAGCGGATG GTGGAGAGGG AGAGACAGAA GATGCGTGTC
    CAGCAGGGTC TGCTGGGCCA CTGCAAGCAT AGTCGCCAGC GGAGCCTCCC AGCCGTCTTC
    TCTCCAGGGA GCAAGGAGGT AACAGAACTT AACCGAGCTG AGAGTTTGTG TCATGAAAAT
    TCATTTTTCA TCAATGAAGC TTTTGGACAC ATGTCACTTA ACACGTCCAA TAAACCAAAC
    CCATCAGGGG TCCCCTGGGA TGCTCACCCC CTGGAGGGGT CTCATTTTGA TTTGGCAAGG
    ACTAGTGAGA GTCCAACAGA ACTGAAGATA GACATTTCTC AGCCTCCTGA TGTCAACTCT
    GAACTCTGGA CAACGGGGCC GGGCCATCAG AGACCTGCTC TCCAAGAAAA CAGCAAGGAG
    TCTTATAAAA ATGTTGCAGA TTTGGACTCC TTCCAGAGTG AATCGTCATC TCCCCAGGAC
    TCCAATCAGC GTGGCCCCCA GCCACAGACA CTCACAACAG AAGCAAAACT AAGTCTTCCG
    ATGGCGGCTG GACACGGTGG CGATGCTGGA GACGGGGCTG AAGAAAACAT TCTTTACCTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu48946
    Clone ID Related Accession (Same CDS sequence) XM_006517518.2 , XM_006517518.3
    Accession Version XM_006517518.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5145bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 28 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006517518.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGGCGGC GACGAGCCCG GCTGGCCAGA GATGCAGGAG CCATGGAGTT GAGCTGCAGT 
    GAAGTGCCTC TTTATGGACA GAAGACGGTC TATGCAAAGT TTGGCAAAAA CGTGTATCTA
    CCCGAAGATG CGGAGTTTTA CTTTGTTTAT GGCGGGTCGC ATCAGAGGCA CGTGGTGATT
    GCTGACCGTG TCCAGGACAA TGTCCTCCAA TCTAGCATTC CAGGCCACTG GCTTCAGGAG
    ACGGTGACGG TATCTGTGTG CCTCTGCTCG GAAGGGTACT CCCCAGTGAC CATGGGCTCT
    GGCTCAGTGA CCTACGTGGA CAACATGGCC TGCAGGCTGG CTCGTCTGCT GGTCACTCAG
    GCTGACCGAC TCACGGCCTG CAGTCACCAG ACGCTGCTGA CCCCGTTTGC CCTGACAGTG
    GAAGCGCTGC CTGCCTTGGA TGAGGAGCTC GTGCTGGCTC TGACCCAACT GGAGCTGCCT
    TTGGGTTGGA CAGTCCTGGG AAATTCCTCA CTTGAAGTAT CTCTACACAG AGAGTCTCTC
    CTGCATCTGG CTGTGAGATG GGCCCTCCCT AAGCTATTCC ACTTCCTCTT GTGTCTCCCT
    GGAGGAGTCA AGGCCTTGAA GCTACCTAAT GAAGAGGCCA CCACACCATT AGACTTAGCT
    TTGCAAGGTG GACACTCCAC ACTGGTAGAA GACATCACAA ATTTTCAGGG TAGCCATTCC
    CCAGGCTTCT CCCGACTGCG TCTTAACGAA GAGGCCACTC TGCAGTTTGT TCATTCATCG
    GAAACATTGA CCCTGACCGT TAACCACACA GCTGAGCATC TCTTGGAAGC AGACATCAAA
    CTCTTCCGGA AATATTTCTG GGACAGAGCC TTTCTTGTCA AGGCTCTTGA GCAAGAAGCC
    AAGACTGAGA AAGCAACCAT GCCTTCTGGA GCTGCAGAAA CTGAAGAAGA GGTTAGGAAT
    TTGGAGTCTG GCAGATCACC ATCGGAGGAG GAGGAGGATG CAAAGAGCAT CAAAAGCCAG
    GTGGATGGGC CGAGTGAACA CGAAGACCAG GACCGCCTAC CTCTGGATCG CTCCTTTGAT
    GGCCTCAAAA AATCCAAGCA TGTCCCAGCG TCCCTTGCTG CAGGCCAGCT CTCTGATGTT
    CTGAACGGGG GTGATGAAGT CTACGCTAAC TGTATGGTGA TTGACCAGGT TGGCGATTTG
    GATATCAACT ATATAAACCT AGAGGGACTC TCCACCCATA CGAGTCCCGA GTCTGGGAGG
    TCCATGCTGG GGCCTCAGGC TTGCATGCAC ACCCTTCCTC CCGACACCAG TCCCTGCGGG
    CGCCCACTCA TTGAGAACAG TGAGGGGACC CTGGATGCTG CAGCTTCGCA GTCTTTCGTG
    ACACCATCCA GTTCTCGCAC TTCCAACTTG AACCTTTCTT TTGGTCTCCA TGGATTTGAA
    AAGGAGCAGA GTCATCTCAA GAAAAGGAGT TCTAGCCTGG ATGCCCTGGT TGCCGACAGT
    GAGGGGGAAG GAGGTTCTGA GCCCCCCATC TGCTATGCTG TGGGCTCTCA GAGTTCCCCA
    AGAACTGGAC TCCCCAGCGG GGATGAACTG GACTCTTTTG AGACCAACAC TGAACCAGAC
    TGTAATATCT CCAGGACCGA ATCACTCTCT CTGTCCAGTA CTCTGCATTC AAAGGAGTCC
    TTGCTTTCTG GGATCCGGTC TCGCTCCTAC TCCTGCTCAT CACCCAAAAT CTCTTCAGGA
    AAATCCCGGC TAGTGCGCGA CTTCACAGTG TGCAGTACCA GCGAAGAACA GAGATCATAT
    AGTTTCCAGG AGCCACCAGG AGAAAAAAGG ATTCAGGAAG AAGAATGGGA TGAATACGTC
    ATTCCTGCCA AATCCGAGTC TGAAAAGTAC AAAGTGAGCC GAACTTTCAG CTTCCTCATG
    AACAGGATGA CCAGCCCACG GAACAAATCT AAGATGAAAA ACAAGGACAC TAAAGAAAAA
    GAAAAAATGA ATCGACATCA GTTTGTCCCG GGAACGTTCT CCGGGGTTCT GCAGTGCTCC
    GGTTGTGATA AAACACTCCT GGGGAAAGAG TCACTCCAGT GTGCCAACTG TAAGGCAAAC
    ACACACAAGG GCTGCAAAGA TGCTGTGCCT CCGTGTACCA AGAAATTCCA AGAGAAATAC
    AACAAGAACA AACCACAGAG CATCCTTGGA AGCTCTTCAG TCAGAGACGT CCCAGCACCT
    GGGCTCTCCT TGCACCCTTC TTCCTCTATG CCCATCGGAC TGCCAGCTGG AAGGAAGGAA
    TTTGCGGCTC AGGTGCATCC ACTGTCCAGA AGTGTCCCCG GCACCACTTT GGAAAGCTTC
    AGGAGGGCAG TGACATCGTT AGAGTCTGAG GGTGACAGCT GGAGAAGCAG GTCTCATTCC
    GATGAGCTGT TCCAGTCTAT GGGCTCTTCT CCTTCCACGG AGTCATTCAT GATGGAAGAT
    GTTGTGGATT CTTCTCTGTG GATCGACCTC AGTAGTGATG CTCAGGAGTT TGAAGCAGAG
    TCTTGGAGTC TCGTGGTGGA CCCTTCCTTT TGCAGTAGGC AGGAGAAGGA TGTCATCAAA
    AGACAAGATG TCATTTTTGA GCTGATGCAG ACAGAGGTCC ACCACATCCA GACTCTGCTC
    ATCATGTCCG AGGTCTTCAG GAAAGGCATG AAGGAGGAAC TGCAGCTGGA CCACAGCACG
    GTGGACAAGA TCTTCCCCTG CTTAGATGAG CTGCTGGAGA CTCACCGGCA CTTCTTCTTC
    AGCATGAAGG AGCGCAGGCA GGAGTCCTGC GCTGGCAGTG ACAGGAACTT TGTGATCAAC
    CAAATTGGAG ACATTCTGGT ACAACAGTTT TCAGAGGAAA ATGCAAGCAA AATGAAGAGG
    ATCTATGGAG AATTCTGCAG CCATCACAAA GAAGCCATGA GTCTCTTTAA AGAGCTGCAG
    CAGAACAAGA AGTTTCAGAA TTTTATTAAG ATCCGAAATA GCAACCTGTT GGCTCGTCGC
    CGCGGAATCC CGGAGTGCAT CCTGCTAGTT ACCCAGCGCA TCACAAAGTA CCCAGTCTTG
    GTGGAAAGGA TCCTGCAGTA CACCAAGGAA AGGACTGAAG AGCACCGGGA CCTGTGCAAA
    GCTCTCGGCT TAATTAAAGA CATGATCGCA GCCGTGGACC TGAAAGTCAG CGAATACGAG
    AAGAACCAAA AATGGCTCGA GATCCTAAAT AAAATCGAGA ACAAAACATA CACAAAGCTC
    AAAAATGGCC ACGTGTTTAG AAAGCAGGCG CTGCTGAGCC AGGAACGGGC TCTCCTGCAT
    GATGGGCTTG TCTACTGGAA AACCGCTACG GGTCGCTTCA AAGACATCCT GGCTCTGCTA
    CTAACTGACG TGCTGCTCTT CTTACAAGAA AAAGACCAGA AATACATCTT TGCAGCTGTT
    GACCAGAAGC CATCAGTGAT TTCCCTCCAG AAGCTGATTG CTAGAGAAGT TGCCAACGAG
    GAGAGAGGCA TGTTTCTGAT CAGTGCTTCG TCAGCCGGCC CCGAGATGTA CGAAATCCAC
    ACCAATTCCA AGGAGGAGCG GAATAACTGG ATGAGGCGGA TCCAGCAGGC AGTAGAAAGT
    TGTCCAGAGG AAGAAGGAGG AAGGACGAGC GAGTCGGATG AAGAGAGGCG GAAAGCTGAA
    GCTAGAGTGG CCAAGATTCA GCAATGTCAA GAAATACTCA GTAACCAAGA CCAACAGATT
    TGCACGTACT TGGAAGAAAA GCTGCATATC TACGCTGAAC TTGGGGAACT GAGTGGCTTT
    GAGGATGTCC ACCTAGAACC CCATCTGCTC ATCAAACCTG ACCCTGGAGA GCCTCCCCAG
    GCAGCGTCCC TGCTTGCAGC AGCCCTGAGA GAAGCCGAGA GTCTGCAAGT GGCTGTGAAG
    GCTTCAAAGA TGGGCGATGT AAGCCAGTCA TCCGAAGAAA GCCCTGGAGG GACTGTCCTG
    ATGGACACAC CCAGTACACA GGATGTACCA GCGTCACCAA CTGCTTCATT AGTCACAGAA
    GGGACAGAAG GAAGAGGCTG TTGGGATGTG GATCCCGGGC TCCAGGGTGT GGTAACCGAC
    TTGGCAGTCT CTGATGCAGG GGAGAAGGTG GAATATAGAA GTTTCTCCGG TTCTTCACAG
    TCAGAGATTA TACAAGCAAT ACAGAATTTA ACGCGTCTCC TCTACAGCCT TCAGGCTGCC
    TTGACCATCC AGGACAGTCA CATTGAGATC CACAAACTGG TTCTACAGCA GCGGGAGAGC
    TTGGCCCCTA GCCACTCCTT CCGAGGTGGC CCCTTGCAAG ACCAGGAGAA ATCCCGCTAC
    CTGGAAAAGC AGCGTGAAGA GCTGGCCAAC ATCCACAAGC TTCAACACCA GTTCCAGCAG
    GAGCAGCGGC GCTGGCACCG CACGTGCGAC CAGCAACAGC GCGAGCAGGA AGCCCAAGAG
    AGCTGGCTGC AGGCGCGGGA ACGTGAGTGC CAGTCCCAGG AGGAATTGTT GCTGCGCCAC
    CGTAGCGAGC TGGACCACCA ACTCCAGGAG TACCAGCAGA GCCTTGAGCG GCTACGGGAG
    GGCCAGCGGA TGGTGGAGAG GGAGAGACAG AAGATGCGTG TCCAGCAGGG TCTGCTGGGC
    CACTGCAAGC ATAGTCGCCA GCGGAGCCTC CCAGCCGTCT TCTCTCCAGG GAGCAAGGAG
    GTAACAGAAC TTAACCGAGC TGAGAGTTTG TGTCATGAAA ATTCATTTTT CATCAATGAA
    GCTTTTGGAC ACATGTCACT TAACACGTCC AATAAACCAA ACCCATCAGG GGTCCCCTGG
    GATGCTCACC CCCTGGAGGG GTCTCATTTT GATTTGGCAA GGACTAGTGA GAGTCCAACA
    GAACTGAAGA TAGACATTTC TCAGCCTCCT GATGTCAACT CTGAACTCTG GACAACGGGG
    CCGGGCCATC AGAGACCTGC TCTCCAAGAA AACAGCAAGG AGTCTTATAA AAATGTTGCA
    GATTTGGACT CCTTCCAGAG TGAATCGTCA TCTCCCCAGG ACTCCAATCA GCGTGGCCCC
    CAGCCACAGA CACTCACAAC AGAAGCAAAA CTAAGTCTTC CGATGGCGGC TGGACACGGT
    GGCGATGCTG GAGACGGGGC TGAAGAAAAC ATTCTTTACC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006517581.1
    CDS261..5405
    Translation

    Target ORF information:

    RefSeq Version XM_006517518.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006517518.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGGCGGC GACGAGCCCG GCTGGCCAGA GATGCAGGAG CCATGGAGTT GAGCTGCAGT 
    GAAGTGCCTC TTTATGGACA GAAGACGGTC TATGCAAAGT TTGGCAAAAA CGTGTATCTA
    CCCGAAGATG CGGAGTTTTA CTTTGTTTAT GGCGGGTCGC ATCAGAGGCA CGTGGTGATT
    GCTGACCGTG TCCAGGACAA TGTCCTCCAA TCTAGCATTC CAGGCCACTG GCTTCAGGAG
    ACGGTGACGG TATCTGTGTG CCTCTGCTCG GAAGGGTACT CCCCAGTGAC CATGGGCTCT
    GGCTCAGTGA CCTACGTGGA CAACATGGCC TGCAGGCTGG CTCGTCTGCT GGTCACTCAG
    GCTGACCGAC TCACGGCCTG CAGTCACCAG ACGCTGCTGA CCCCGTTTGC CCTGACAGTG
    GAAGCGCTGC CTGCCTTGGA TGAGGAGCTC GTGCTGGCTC TGACCCAACT GGAGCTGCCT
    TTGGGTTGGA CAGTCCTGGG AAATTCCTCA CTTGAAGTAT CTCTACACAG AGAGTCTCTC
    CTGCATCTGG CTGTGAGATG GGCCCTCCCT AAGCTATTCC ACTTCCTCTT GTGTCTCCCT
    GGAGGAGTCA AGGCCTTGAA GCTACCTAAT GAAGAGGCCA CCACACCATT AGACTTAGCT
    TTGCAAGGTG GACACTCCAC ACTGGTAGAA GACATCACAA ATTTTCAGGG TAGCCATTCC
    CCAGGCTTCT CCCGACTGCG TCTTAACGAA GAGGCCACTC TGCAGTTTGT TCATTCATCG
    GAAACATTGA CCCTGACCGT TAACCACACA GCTGAGCATC TCTTGGAAGC AGACATCAAA
    CTCTTCCGGA AATATTTCTG GGACAGAGCC TTTCTTGTCA AGGCTCTTGA GCAAGAAGCC
    AAGACTGAGA AAGCAACCAT GCCTTCTGGA GCTGCAGAAA CTGAAGAAGA GGTTAGGAAT
    TTGGAGTCTG GCAGATCACC ATCGGAGGAG GAGGAGGATG CAAAGAGCAT CAAAAGCCAG
    GTGGATGGGC CGAGTGAACA CGAAGACCAG GACCGCCTAC CTCTGGATCG CTCCTTTGAT
    GGCCTCAAAA AATCCAAGCA TGTCCCAGCG TCCCTTGCTG CAGGCCAGCT CTCTGATGTT
    CTGAACGGGG GTGATGAAGT CTACGCTAAC TGTATGGTGA TTGACCAGGT TGGCGATTTG
    GATATCAACT ATATAAACCT AGAGGGACTC TCCACCCATA CGAGTCCCGA GTCTGGGAGG
    TCCATGCTGG GGCCTCAGGC TTGCATGCAC ACCCTTCCTC CCGACACCAG TCCCTGCGGG
    CGCCCACTCA TTGAGAACAG TGAGGGGACC CTGGATGCTG CAGCTTCGCA GTCTTTCGTG
    ACACCATCCA GTTCTCGCAC TTCCAACTTG AACCTTTCTT TTGGTCTCCA TGGATTTGAA
    AAGGAGCAGA GTCATCTCAA GAAAAGGAGT TCTAGCCTGG ATGCCCTGGT TGCCGACAGT
    GAGGGGGAAG GAGGTTCTGA GCCCCCCATC TGCTATGCTG TGGGCTCTCA GAGTTCCCCA
    AGAACTGGAC TCCCCAGCGG GGATGAACTG GACTCTTTTG AGACCAACAC TGAACCAGAC
    TGTAATATCT CCAGGACCGA ATCACTCTCT CTGTCCAGTA CTCTGCATTC AAAGGAGTCC
    TTGCTTTCTG GGATCCGGTC TCGCTCCTAC TCCTGCTCAT CACCCAAAAT CTCTTCAGGA
    AAATCCCGGC TAGTGCGCGA CTTCACAGTG TGCAGTACCA GCGAAGAACA GAGATCATAT
    AGTTTCCAGG AGCCACCAGG AGAAAAAAGG ATTCAGGAAG AAGAATGGGA TGAATACGTC
    ATTCCTGCCA AATCCGAGTC TGAAAAGTAC AAAGTGAGCC GAACTTTCAG CTTCCTCATG
    AACAGGATGA CCAGCCCACG GAACAAATCT AAGATGAAAA ACAAGGACAC TAAAGAAAAA
    GAAAAAATGA ATCGACATCA GTTTGTCCCG GGAACGTTCT CCGGGGTTCT GCAGTGCTCC
    GGTTGTGATA AAACACTCCT GGGGAAAGAG TCACTCCAGT GTGCCAACTG TAAGGCAAAC
    ACACACAAGG GCTGCAAAGA TGCTGTGCCT CCGTGTACCA AGAAATTCCA AGAGAAATAC
    AACAAGAACA AACCACAGAG CATCCTTGGA AGCTCTTCAG TCAGAGACGT CCCAGCACCT
    GGGCTCTCCT TGCACCCTTC TTCCTCTATG CCCATCGGAC TGCCAGCTGG AAGGAAGGAA
    TTTGCGGCTC AGGTGCATCC ACTGTCCAGA AGTGTCCCCG GCACCACTTT GGAAAGCTTC
    AGGAGGGCAG TGACATCGTT AGAGTCTGAG GGTGACAGCT GGAGAAGCAG GTCTCATTCC
    GATGAGCTGT TCCAGTCTAT GGGCTCTTCT CCTTCCACGG AGTCATTCAT GATGGAAGAT
    GTTGTGGATT CTTCTCTGTG GATCGACCTC AGTAGTGATG CTCAGGAGTT TGAAGCAGAG
    TCTTGGAGTC TCGTGGTGGA CCCTTCCTTT TGCAGTAGGC AGGAGAAGGA TGTCATCAAA
    AGACAAGATG TCATTTTTGA GCTGATGCAG ACAGAGGTCC ACCACATCCA GACTCTGCTC
    ATCATGTCCG AGGTCTTCAG GAAAGGCATG AAGGAGGAAC TGCAGCTGGA CCACAGCACG
    GTGGACAAGA TCTTCCCCTG CTTAGATGAG CTGCTGGAGA CTCACCGGCA CTTCTTCTTC
    AGCATGAAGG AGCGCAGGCA GGAGTCCTGC GCTGGCAGTG ACAGGAACTT TGTGATCAAC
    CAAATTGGAG ACATTCTGGT ACAACAGTTT TCAGAGGAAA ATGCAAGCAA AATGAAGAGG
    ATCTATGGAG AATTCTGCAG CCATCACAAA GAAGCCATGA GTCTCTTTAA AGAGCTGCAG
    CAGAACAAGA AGTTTCAGAA TTTTATTAAG ATCCGAAATA GCAACCTGTT GGCTCGTCGC
    CGCGGAATCC CGGAGTGCAT CCTGCTAGTT ACCCAGCGCA TCACAAAGTA CCCAGTCTTG
    GTGGAAAGGA TCCTGCAGTA CACCAAGGAA AGGACTGAAG AGCACCGGGA CCTGTGCAAA
    GCTCTCGGCT TAATTAAAGA CATGATCGCA GCCGTGGACC TGAAAGTCAG CGAATACGAG
    AAGAACCAAA AATGGCTCGA GATCCTAAAT AAAATCGAGA ACAAAACATA CACAAAGCTC
    AAAAATGGCC ACGTGTTTAG AAAGCAGGCG CTGCTGAGCC AGGAACGGGC TCTCCTGCAT
    GATGGGCTTG TCTACTGGAA AACCGCTACG GGTCGCTTCA AAGACATCCT GGCTCTGCTA
    CTAACTGACG TGCTGCTCTT CTTACAAGAA AAAGACCAGA AATACATCTT TGCAGCTGTT
    GACCAGAAGC CATCAGTGAT TTCCCTCCAG AAGCTGATTG CTAGAGAAGT TGCCAACGAG
    GAGAGAGGCA TGTTTCTGAT CAGTGCTTCG TCAGCCGGCC CCGAGATGTA CGAAATCCAC
    ACCAATTCCA AGGAGGAGCG GAATAACTGG ATGAGGCGGA TCCAGCAGGC AGTAGAAAGT
    TGTCCAGAGG AAGAAGGAGG AAGGACGAGC GAGTCGGATG AAGAGAGGCG GAAAGCTGAA
    GCTAGAGTGG CCAAGATTCA GCAATGTCAA GAAATACTCA GTAACCAAGA CCAACAGATT
    TGCACGTACT TGGAAGAAAA GCTGCATATC TACGCTGAAC TTGGGGAACT GAGTGGCTTT
    GAGGATGTCC ACCTAGAACC CCATCTGCTC ATCAAACCTG ACCCTGGAGA GCCTCCCCAG
    GCAGCGTCCC TGCTTGCAGC AGCCCTGAGA GAAGCCGAGA GTCTGCAAGT GGCTGTGAAG
    GCTTCAAAGA TGGGCGATGT AAGCCAGTCA TCCGAAGAAA GCCCTGGAGG GACTGTCCTG
    ATGGACACAC CCAGTACACA GGATGTACCA GCGTCACCAA CTGCTTCATT AGTCACAGAA
    GGGACAGAAG GAAGAGGCTG TTGGGATGTG GATCCCGGGC TCCAGGGTGT GGTAACCGAC
    TTGGCAGTCT CTGATGCAGG GGAGAAGGTG GAATATAGAA GTTTCTCCGG TTCTTCACAG
    TCAGAGATTA TACAAGCAAT ACAGAATTTA ACGCGTCTCC TCTACAGCCT TCAGGCTGCC
    TTGACCATCC AGGACAGTCA CATTGAGATC CACAAACTGG TTCTACAGCA GCGGGAGAGC
    TTGGCCCCTA GCCACTCCTT CCGAGGTGGC CCCTTGCAAG ACCAGGAGAA ATCCCGCTAC
    CTGGAAAAGC AGCGTGAAGA GCTGGCCAAC ATCCACAAGC TTCAACACCA GTTCCAGCAG
    GAGCAGCGGC GCTGGCACCG CACGTGCGAC CAGCAACAGC GCGAGCAGGA AGCCCAAGAG
    AGCTGGCTGC AGGCGCGGGA ACGTGAGTGC CAGTCCCAGG AGGAATTGTT GCTGCGCCAC
    CGTAGCGAGC TGGACCACCA ACTCCAGGAG TACCAGCAGA GCCTTGAGCG GCTACGGGAG
    GGCCAGCGGA TGGTGGAGAG GGAGAGACAG AAGATGCGTG TCCAGCAGGG TCTGCTGGGC
    CACTGCAAGC ATAGTCGCCA GCGGAGCCTC CCAGCCGTCT TCTCTCCAGG GAGCAAGGAG
    GTAACAGAAC TTAACCGAGC TGAGAGTTTG TGTCATGAAA ATTCATTTTT CATCAATGAA
    GCTTTTGGAC ACATGTCACT TAACACGTCC AATAAACCAA ACCCATCAGG GGTCCCCTGG
    GATGCTCACC CCCTGGAGGG GTCTCATTTT GATTTGGCAA GGACTAGTGA GAGTCCAACA
    GAACTGAAGA TAGACATTTC TCAGCCTCCT GATGTCAACT CTGAACTCTG GACAACGGGG
    CCGGGCCATC AGAGACCTGC TCTCCAAGAA AACAGCAAGG AGTCTTATAA AAATGTTGCA
    GATTTGGACT CCTTCCAGAG TGAATCGTCA TCTCCCCAGG ACTCCAATCA GCGTGGCCCC
    CAGCCACAGA CACTCACAAC AGAAGCAAAA CTAAGTCTTC CGATGGCGGC TGGACACGGT
    GGCGATGCTG GAGACGGGGC TGAAGAAAAC ATTCTTTACC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu48947
    Clone ID Related Accession (Same CDS sequence) XM_006517519.4 , XM_006517519.3
    Accession Version XM_006517519.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4164bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 28 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517519.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGATTCTG ACTCGGACTC ACCTTTCAAC TACTCCTGGC CTTCCTTCCC CAAGATGAAG 
    ATTCGGCGCA GGGCATCCAA GAAAGATCGC TCCTTTGATG GCCTCAAAAA ATCCAAGCAT
    GTCCCAGCGT CCCTTGCTGC AGGCCAGCTC TCTGATGTTC TGAACGGGGG TGATGAAGTC
    TACGCTAACT GTATGGTGAT TGACCAGGTT GGCGATTTGG ATATCAACTA TATAAACCTA
    GAGGGACTCT CCACCCATAC GAGTCCCGAG TCTGGGAGGT CCATGCTGGG GCCTCAGGCT
    TGCATGCACA CCCTTCCTCC CGACACCAGT CCCTGCGGGC GCCCACTCAT TGAGAACAGT
    GAGGGGACCC TGGATGCTGC AGCTTCGCAG TCTTTCGTGA CACCATCCAG TTCTCGCACT
    TCCAACTTGA ACCTTTCTTT TGGTCTCCAT GGATTTGAAA AGGAGCAGAG TCATCTCAAG
    AAAAGGAGTT CTAGCCTGGA TGCCCTGGTT GCCGACAGTG AGGGGGAAGG AGGTTCTGAG
    CCCCCCATCT GCTATGCTGT GGGCTCTCAG AGTTCCCCAA GAACTGGACT CCCCAGCGGG
    GATGAACTGG ACTCTTTTGA GACCAACACT GAACCAGACT GTAATATCTC CAGGACCGAA
    TCACTCTCTC TGTCCAGTAC TCTGCATTCA AAGGAGTCCT TGCTTTCTGG GATCCGGTCT
    CGCTCCTACT CCTGCTCATC ACCCAAAATC TCTTCAGGAA AATCCCGGCT AGTGCGCGAC
    TTCACAGTGT GCAGTACCAG CGAAGAACAG AGATCATATA GTTTCCAGGA GCCACCAGGA
    GAAAAAAGGA TTCAGGAAGA AGAATGGGAT GAATACGTCA TTCCTGCCAA ATCCGAGTCT
    GAAAAGTACA AAGTGAGCCG AACTTTCAGC TTCCTCATGA ACAGGATGAC CAGCCCACGG
    AACAAATCTA AGATGAAAAA CAAGGACACT AAAGAAAAAG AAAAAATGAA TCGACATCAG
    TTTGTCCCGG GAACGTTCTC CGGGGTTCTG CAGTGCTCCG GTTGTGATAA AACACTCCTG
    GGGAAAGAGT CACTCCAGTG TGCCAACTGT AAGGCAAACA CACACAAGGG CTGCAAAGAT
    GCTGTGCCTC CGTGTACCAA GAAATTCCAA GAGAAATACA ACAAGAACAA ACCACAGAGC
    ATCCTTGGAA GCTCTTCAGT CAGAGACGTC CCAGCACCTG GGCTCTCCTT GCACCCTTCT
    TCCTCTATGC CCATCGGACT GCCAGCTGGA AGGAAGGAAT TTGCGGCTCA GGTGCATCCA
    CTGTCCAGAA GTGTCCCCGG CACCACTTTG GAAAGCTTCA GGAGGGCAGT GACATCGTTA
    GAGTCTGAGG GTGACAGCTG GAGAAGCAGG TCTCATTCCG ATGAGCTGTT CCAGTCTATG
    GGCTCTTCTC CTTCCACGGA GTCATTCATG ATGGAAGATG TTGTGGATTC TTCTCTGTGG
    ATCGACCTCA GTAGTGATGC TCAGGAGTTT GAAGCAGAGT CTTGGAGTCT CGTGGTGGAC
    CCTTCCTTTT GCAGTAGGCA GGAGAAGGAT GTCATCAAAA GACAAGATGT CATTTTTGAG
    CTGATGCAGA CAGAGGTCCA CCACATCCAG ACTCTGCTCA TCATGTCCGA GGTCTTCAGG
    AAAGGCATGA AGGAGGAACT GCAGCTGGAC CACAGCACGG TGGACAAGAT CTTCCCCTGC
    TTAGATGAGC TGCTGGAGAC TCACCGGCAC TTCTTCTTCA GCATGAAGGA GCGCAGGCAG
    GAGTCCTGCG CTGGCAGTGA CAGGAACTTT GTGATCAACC AAATTGGAGA CATTCTGGTA
    CAACAGTTTT CAGAGGAAAA TGCAAGCAAA ATGAAGAGGA TCTATGGAGA ATTCTGCAGC
    CATCACAAAG AAGCCATGAG TCTCTTTAAA GAGCTGCAGC AGAACAAGAA GTTTCAGAAT
    TTTATTAAGA TCCGAAATAG CAACCTGTTG GCTCGTCGCC GCGGAATCCC GGAGTGCATC
    CTGCTAGTTA CCCAGCGCAT CACAAAGTAC CCAGTCTTGG TGGAAAGGAT CCTGCAGTAC
    ACCAAGGAAA GGACTGAAGA GCACCGGGAC CTGTGCAAAG CTCTCGGCTT AATTAAAGAC
    ATGATCGCAG CCGTGGACCT GAAAGTCAGC GAATACGAGA AGAACCAAAA ATGGCTCGAG
    ATCCTAAATA AAATCGAGAA CAAAACATAC ACAAAGCTCA AAAATGGCCA CGTGTTTAGA
    AAGCAGGCGC TGCTGAGCCA GGAACGGGCT CTCCTGCATG ATGGGCTTGT CTACTGGAAA
    ACCGCTACGG GTCGCTTCAA AGACATCCTG GCTCTGCTAC TAACTGACGT GCTGCTCTTC
    TTACAAGAAA AAGACCAGAA ATACATCTTT GCAGCTGTTG ACCAGAAGCC ATCAGTGATT
    TCCCTCCAGA AGCTGATTGC TAGAGAAGTT GCCAACGAGG AGAGAGGCAT GTTTCTGATC
    AGTGCTTCGT CAGCCGGCCC CGAGATGTAC GAAATCCACA CCAATTCCAA GGAGGAGCGG
    AATAACTGGA TGAGGCGGAT CCAGCAGGCA GTAGAAAGTT GTCCAGAGGA AGAAGGAGGA
    AGGACGAGCG AGTCGGATGA AGAGAGGCGG AAAGCTGAAG CTAGAGTGGC CAAGATTCAG
    CAATGTCAAG AAATACTCAG TAACCAAGAC CAACAGATTT GCACGTACTT GGAAGAAAAG
    CTGCATATCT ACGCTGAACT TGGGGAACTG AGTGGCTTTG AGGATGTCCA CCTAGAACCC
    CATCTGCTCA TCAAACCTGA CCCTGGAGAG CCTCCCCAGG CAGCGTCCCT GCTTGCAGCA
    GCCCTGAGAG AAGCCGAGAG TCTGCAAGTG GCTGTGAAGG CTTCAAAGAT GGGCGATGTA
    AGCCAGTCAT CCGAAGAAAG CCCTGGAGGG ACTGTCCTGA TGGACACACC CAGTACACAG
    GATGTACCAG CGTCACCAAC TGCTTCATTA GTCACAGAAG GGACAGAAGG AAGAGGCTGT
    TGGGATGTGG ATCCCGGGCT CCAGGGTGTG GTAACCGACT TGGCAGTCTC TGATGCAGGG
    GAGAAGGTGG AATATAGAAG TTTCTCCGGT TCTTCACAGT CAGAGATTAT ACAAGCAATA
    CAGAATTTAA CGCGTCTCCT CTACAGCCTT CAGGCTGCCT TGACCATCCA GGACAGTCAC
    ATTGAGATCC ACAAACTGGT TCTACAGCAG CGGGAGAGCT TGGCCCCTAG CCACTCCTTC
    CGAGGTGGCC CCTTGCAAGA CCAGGAGAAA TCCCGCTACC TGGAAAAGCA GCGTGAAGAG
    CTGGCCAACA TCCACAAGCT TCAACACCAG TTCCAGCAGG AGCAGCGGCG CTGGCACCGC
    ACGTGCGACC AGCAACAGCG CGAGCAGGAA GCCCAAGAGA GCTGGCTGCA GGCGCGGGAA
    CGTGAGTGCC AGTCCCAGGA GGAATTGTTG CTGCGCCACC GTAGCGAGCT GGACCACCAA
    CTCCAGGAGT ACCAGCAGAG CCTTGAGCGG CTACGGGAGG GCCAGCGGAT GGTGGAGAGG
    GAGAGACAGA AGATGCGTGT CCAGCAGGGT CTGCTGGGCC ACTGCAAGCA TAGTCGCCAG
    CGGAGCCTCC CAGCCGTCTT CTCTCCAGGG AGCAAGGAGG TAACAGAACT TAACCGAGCT
    GAGAGTTTGT GTCATGAAAA TTCATTTTTC ATCAATGAAG CTTTTGGACA CATGTCACTT
    AACACGTCCA ATAAACCAAA CCCATCAGGG GTCCCCTGGG ATGCTCACCC CCTGGAGGGG
    TCTCATTTTG ATTTGGCAAG GACTAGTGAG AGTCCAACAG AACTGAAGAT AGACATTTCT
    CAGCCTCCTG ATGTCAACTC TGAACTCTGG ACAACGGGGC CGGGCCATCA GAGACCTGCT
    CTCCAAGAAA ACAGCAAGGA GTCTTATAAA AATGTTGCAG ATTTGGACTC CTTCCAGAGT
    GAATCGTCAT CTCCCCAGGA CTCCAATCAG CGTGGCCCCC AGCCACAGAC ACTCACAACA
    GAAGCAAAAC TAAGTCTTCC GATGGCGGCT GGACACGGTG GCGATGCTGG AGACGGGGCT
    GAAGAAAACA TTCTTTACCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006517582.1
    CDS1485..5648
    Translation

    Target ORF information:

    RefSeq Version XM_006517519.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006517519.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGATTCTG ACTCGGACTC ACCTTTCAAC TACTCCTGGC CTTCCTTCCC CAAGATGAAG 
    ATTCGGCGCA GGGCATCCAA GAAAGATCGC TCCTTTGATG GCCTCAAAAA ATCCAAGCAT
    GTCCCAGCGT CCCTTGCTGC AGGCCAGCTC TCTGATGTTC TGAACGGGGG TGATGAAGTC
    TACGCTAACT GTATGGTGAT TGACCAGGTT GGCGATTTGG ATATCAACTA TATAAACCTA
    GAGGGACTCT CCACCCATAC GAGTCCCGAG TCTGGGAGGT CCATGCTGGG GCCTCAGGCT
    TGCATGCACA CCCTTCCTCC CGACACCAGT CCCTGCGGGC GCCCACTCAT TGAGAACAGT
    GAGGGGACCC TGGATGCTGC AGCTTCGCAG TCTTTCGTGA CACCATCCAG TTCTCGCACT
    TCCAACTTGA ACCTTTCTTT TGGTCTCCAT GGATTTGAAA AGGAGCAGAG TCATCTCAAG
    AAAAGGAGTT CTAGCCTGGA TGCCCTGGTT GCCGACAGTG AGGGGGAAGG AGGTTCTGAG
    CCCCCCATCT GCTATGCTGT GGGCTCTCAG AGTTCCCCAA GAACTGGACT CCCCAGCGGG
    GATGAACTGG ACTCTTTTGA GACCAACACT GAACCAGACT GTAATATCTC CAGGACCGAA
    TCACTCTCTC TGTCCAGTAC TCTGCATTCA AAGGAGTCCT TGCTTTCTGG GATCCGGTCT
    CGCTCCTACT CCTGCTCATC ACCCAAAATC TCTTCAGGAA AATCCCGGCT AGTGCGCGAC
    TTCACAGTGT GCAGTACCAG CGAAGAACAG AGATCATATA GTTTCCAGGA GCCACCAGGA
    GAAAAAAGGA TTCAGGAAGA AGAATGGGAT GAATACGTCA TTCCTGCCAA ATCCGAGTCT
    GAAAAGTACA AAGTGAGCCG AACTTTCAGC TTCCTCATGA ACAGGATGAC CAGCCCACGG
    AACAAATCTA AGATGAAAAA CAAGGACACT AAAGAAAAAG AAAAAATGAA TCGACATCAG
    TTTGTCCCGG GAACGTTCTC CGGGGTTCTG CAGTGCTCCG GTTGTGATAA AACACTCCTG
    GGGAAAGAGT CACTCCAGTG TGCCAACTGT AAGGCAAACA CACACAAGGG CTGCAAAGAT
    GCTGTGCCTC CGTGTACCAA GAAATTCCAA GAGAAATACA ACAAGAACAA ACCACAGAGC
    ATCCTTGGAA GCTCTTCAGT CAGAGACGTC CCAGCACCTG GGCTCTCCTT GCACCCTTCT
    TCCTCTATGC CCATCGGACT GCCAGCTGGA AGGAAGGAAT TTGCGGCTCA GGTGCATCCA
    CTGTCCAGAA GTGTCCCCGG CACCACTTTG GAAAGCTTCA GGAGGGCAGT GACATCGTTA
    GAGTCTGAGG GTGACAGCTG GAGAAGCAGG TCTCATTCCG ATGAGCTGTT CCAGTCTATG
    GGCTCTTCTC CTTCCACGGA GTCATTCATG ATGGAAGATG TTGTGGATTC TTCTCTGTGG
    ATCGACCTCA GTAGTGATGC TCAGGAGTTT GAAGCAGAGT CTTGGAGTCT CGTGGTGGAC
    CCTTCCTTTT GCAGTAGGCA GGAGAAGGAT GTCATCAAAA GACAAGATGT CATTTTTGAG
    CTGATGCAGA CAGAGGTCCA CCACATCCAG ACTCTGCTCA TCATGTCCGA GGTCTTCAGG
    AAAGGCATGA AGGAGGAACT GCAGCTGGAC CACAGCACGG TGGACAAGAT CTTCCCCTGC
    TTAGATGAGC TGCTGGAGAC TCACCGGCAC TTCTTCTTCA GCATGAAGGA GCGCAGGCAG
    GAGTCCTGCG CTGGCAGTGA CAGGAACTTT GTGATCAACC AAATTGGAGA CATTCTGGTA
    CAACAGTTTT CAGAGGAAAA TGCAAGCAAA ATGAAGAGGA TCTATGGAGA ATTCTGCAGC
    CATCACAAAG AAGCCATGAG TCTCTTTAAA GAGCTGCAGC AGAACAAGAA GTTTCAGAAT
    TTTATTAAGA TCCGAAATAG CAACCTGTTG GCTCGTCGCC GCGGAATCCC GGAGTGCATC
    CTGCTAGTTA CCCAGCGCAT CACAAAGTAC CCAGTCTTGG TGGAAAGGAT CCTGCAGTAC
    ACCAAGGAAA GGACTGAAGA GCACCGGGAC CTGTGCAAAG CTCTCGGCTT AATTAAAGAC
    ATGATCGCAG CCGTGGACCT GAAAGTCAGC GAATACGAGA AGAACCAAAA ATGGCTCGAG
    ATCCTAAATA AAATCGAGAA CAAAACATAC ACAAAGCTCA AAAATGGCCA CGTGTTTAGA
    AAGCAGGCGC TGCTGAGCCA GGAACGGGCT CTCCTGCATG ATGGGCTTGT CTACTGGAAA
    ACCGCTACGG GTCGCTTCAA AGACATCCTG GCTCTGCTAC TAACTGACGT GCTGCTCTTC
    TTACAAGAAA AAGACCAGAA ATACATCTTT GCAGCTGTTG ACCAGAAGCC ATCAGTGATT
    TCCCTCCAGA AGCTGATTGC TAGAGAAGTT GCCAACGAGG AGAGAGGCAT GTTTCTGATC
    AGTGCTTCGT CAGCCGGCCC CGAGATGTAC GAAATCCACA CCAATTCCAA GGAGGAGCGG
    AATAACTGGA TGAGGCGGAT CCAGCAGGCA GTAGAAAGTT GTCCAGAGGA AGAAGGAGGA
    AGGACGAGCG AGTCGGATGA AGAGAGGCGG AAAGCTGAAG CTAGAGTGGC CAAGATTCAG
    CAATGTCAAG AAATACTCAG TAACCAAGAC CAACAGATTT GCACGTACTT GGAAGAAAAG
    CTGCATATCT ACGCTGAACT TGGGGAACTG AGTGGCTTTG AGGATGTCCA CCTAGAACCC
    CATCTGCTCA TCAAACCTGA CCCTGGAGAG CCTCCCCAGG CAGCGTCCCT GCTTGCAGCA
    GCCCTGAGAG AAGCCGAGAG TCTGCAAGTG GCTGTGAAGG CTTCAAAGAT GGGCGATGTA
    AGCCAGTCAT CCGAAGAAAG CCCTGGAGGG ACTGTCCTGA TGGACACACC CAGTACACAG
    GATGTACCAG CGTCACCAAC TGCTTCATTA GTCACAGAAG GGACAGAAGG AAGAGGCTGT
    TGGGATGTGG ATCCCGGGCT CCAGGGTGTG GTAACCGACT TGGCAGTCTC TGATGCAGGG
    GAGAAGGTGG AATATAGAAG TTTCTCCGGT TCTTCACAGT CAGAGATTAT ACAAGCAATA
    CAGAATTTAA CGCGTCTCCT CTACAGCCTT CAGGCTGCCT TGACCATCCA GGACAGTCAC
    ATTGAGATCC ACAAACTGGT TCTACAGCAG CGGGAGAGCT TGGCCCCTAG CCACTCCTTC
    CGAGGTGGCC CCTTGCAAGA CCAGGAGAAA TCCCGCTACC TGGAAAAGCA GCGTGAAGAG
    CTGGCCAACA TCCACAAGCT TCAACACCAG TTCCAGCAGG AGCAGCGGCG CTGGCACCGC
    ACGTGCGACC AGCAACAGCG CGAGCAGGAA GCCCAAGAGA GCTGGCTGCA GGCGCGGGAA
    CGTGAGTGCC AGTCCCAGGA GGAATTGTTG CTGCGCCACC GTAGCGAGCT GGACCACCAA
    CTCCAGGAGT ACCAGCAGAG CCTTGAGCGG CTACGGGAGG GCCAGCGGAT GGTGGAGAGG
    GAGAGACAGA AGATGCGTGT CCAGCAGGGT CTGCTGGGCC ACTGCAAGCA TAGTCGCCAG
    CGGAGCCTCC CAGCCGTCTT CTCTCCAGGG AGCAAGGAGG TAACAGAACT TAACCGAGCT
    GAGAGTTTGT GTCATGAAAA TTCATTTTTC ATCAATGAAG CTTTTGGACA CATGTCACTT
    AACACGTCCA ATAAACCAAA CCCATCAGGG GTCCCCTGGG ATGCTCACCC CCTGGAGGGG
    TCTCATTTTG ATTTGGCAAG GACTAGTGAG AGTCCAACAG AACTGAAGAT AGACATTTCT
    CAGCCTCCTG ATGTCAACTC TGAACTCTGG ACAACGGGGC CGGGCCATCA GAGACCTGCT
    CTCCAAGAAA ACAGCAAGGA GTCTTATAAA AATGTTGCAG ATTTGGACTC CTTCCAGAGT
    GAATCGTCAT CTCCCCAGGA CTCCAATCAG CGTGGCCCCC AGCCACAGAC ACTCACAACA
    GAAGCAAAAC TAAGTCTTCC GATGGCGGCT GGACACGGTG GCGATGCTGG AGACGGGGCT
    GAAGAAAACA TTCTTTACCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu48949
    Clone ID Related Accession (Same CDS sequence) XM_006517521.3 , XM_006517521.4
    Accession Version XM_006517521.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 28 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517521.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGGCGGC GACGAGCCCG GCTGGCCAGA GATGCAGGAG CCATGGAGTT GAGCTGCAGT 
    GAAGTGCCTC TTTATGGACA GAAGACGGTC TATGCAAAGT TTGGCAAAAA CGTGTATCTA
    CCCGAAGATG CGGAGTTTTA CTTTGTTTAT GGCGGGTCGC ATCAGAGGCA CGTGGTGATT
    GCTGACCGTG TCCAGGACAA TGTCCTCCAA TCTAGCATTC CAGGCCACTG GCTTCAGGAG
    ACGGTGACGG TATCTGTGTG CCTCTGCTCG GAAGGGTACT CCCCAGTGAC CATGGGCTCT
    GGCTCAGTGA CCTACGTGGA CAACATGGCC TGCAGGCTGG CTCGTCTGCT GGTCACTCAG
    GCTGACCGAC TCACGGCCTG CAGTCACCAG ACGCTGCTGA CCCCGTTTGC CCTGACAGTG
    GAAGCGCTGC CTGCCTTGGA TGAGGAGCTC GTGCTGGCTC TGACCCAACT GGAGCTGCCT
    TTGGGTTGGA CAGTCCTGGG AAATTCCTCA CTTGAAGTAT CTCTACACAG AGAGTCTCTC
    CTGCATCTGG CTGTGAGATG GGCCCTCCCT AAGCTATTCC ACTTCCTCTT GTGTCTCCCT
    GGAGGAGTCA AGGCCTTGAA GCTACCTAAT GAAGAGGCCA CCACACCATT AGACTTAGCT
    TTGCAAGGTG GACACTCCAC ACTGGTAGAA GACATCACAA ATTTTCAGGG TAGCCATTCC
    CCAGGCTTCT CCCGACTGCG TCTTAACGAA GAGGCCACTC TGCAGTTTGT TCATTCATCG
    GAAACATTGA CCCTGACCGT TAACCACACA GCTGAGCATC TCTTGGAAGC AGACATCAAA
    CTCTTCCGGA AATATTTCTG GGACAGAGCC TTTCTTGTCA AGGCTCTTGA GCAAGAAGCC
    AAGACTGAGA AAGCAACCAT GCCTTCTGGA GCTGCAGAAA CTGAAGAAGA GGTTAGGAAT
    TTGGAGTCTG GCAGATCACC ATCGGAGGAG GAGGAGGATG CAAAGAGCAT CAAAAGCCAG
    GTGGATGGGC CGAGTGAACA CGAAGACCAG GACCGCCTAC CTCTGGATCG CTCCTTTGAT
    GGCCTCAAAA AATCCAAGCA TGTCCCAGCG TCCCTTGCTG CAGGCCAGCT CTCTGATGTT
    CTGAACGGGG GTGATGAAGT CTACGCTAAC TGTATGGTGA TTGACCAGGT TGGCGATTTG
    GATATCAACT ATATAAACCT AGAGGGACTC TCCACCCATA CGAGTCCCGA GTCTGGGAGG
    TCCATGCTGG GGCCTCAGGC TTGCATGCAC ACCCTTCCTC CCGACACCAG TCCCTGCGGG
    CGCCCACTCA TTGAGAACAG TGAGGGGACC CTGGATGCTG CAGCTTCGCA GTCTTTCGTG
    ACACCATCCA GTTCTCGCAC TTCCAACTTG AACCTTTCTT TTGGTCTCCA TGGATTTGAA
    AAGGAGCAGA GTCATCTCAA GAAAAGGAGT TCTAGCCTGG ATGCCCTGGT TGCCGACAGT
    GAGGGGGAAG GAGGTTCTGA GCCCCCCATC TGCTATGCTG TGGGCTCTCA GAGTTCCCCA
    AGAACTGGAC TCCCCAGCGG GGATGAACTG GACTCTTTTG AGACCAACAC TGAACCAGAC
    TGTAATATCT CCAGGACCGA ATCACTCTCT CTGTCCAGTA CTCTGCATTC AAAGGAGTCC
    TTGCTTTCTG GGATCCGGTC TCGCTCCTAC TCCTGCTCAT CACCCAAAAT CTCTTCAGGA
    AAATCCCGGC TAGTGCGCGA CTTCACAGTG TGCAGTACCA GCGAAGAACA GAGATCATAT
    AGTTTCCAGG AGCCACCAGG AGAAAAAAGG ATTCAGGAAG AAGAATGGGA TGAATACGTC
    ATTCCTGCCA AATCCGAGTC TGAAAAGTAC AAAGTGAGCC GAACTTTCAG CTTCCTCATG
    AACAGGATGA CCAGCCCACG GAACAAATCT AAGATGAAAA ACAAGGACAC TAAAGAAAAA
    GAAAAAATGA ATCGACATCA GTTTGTCCCG GGAACGTTCT CCGGGGTTCT GCAGTGCTCC
    GGTTGTGATA AAACACTCCT GGGGAAAGAG TCACTCCAGT GTGCCAACTG TAAGGCAAAC
    ACACACAAGG GCTGCAAAGA TGCTGTGCCT CCGTGTACCA AGAAATTCCA AGAGAAATAC
    AACAAGAACA AACCACAGAG CATCCTTGGA AGCTCTTCAG TCAGAGACGT CCCAGCACCT
    GGGCTCTCCT TGCACCCTTC TTCCTCTATG CCCATCGGAC TGCCAGCTGG AAGGAAGGAA
    TTTGCGGCTC AGGTGCATCC ACTGTCCAGA AGTGTCCCCG GCACCACTTT GGAAAGCTTC
    AGGAGGGCAG TGACATCGTT AGAGTCTGAG GGTGACAGCT GGAGAAGCAG GTCTCATTCC
    GATGAGCTGT TCCAGTCTAT GGGCTCTTCT CCTTCCACGG AGTCATTCAT GATGGAAGAT
    GTTGTGGATT CTTCTCTGTG GATCGACCTC AGTAGTGATG CTCAGGAGTT TGAAGCAGAG
    TCTTGGAGTC TCGTGGTGGA CCCTTCCTTT TGCAGTAGGC AGGAGAAGGA TGTCATCAAA
    AGACAAGATG TCATTTTTGA GCTGATGCAG ACAGAGGTCC ACCACATCCA GACTCTGCTC
    ATCATGTCCG AGGTCTTCAG GAAAGGCATG AAGGAGGAAC TGCAGCTGGA CCACAGCACG
    GTGGACAAGA TCTTCCCCTG CTTAGATGAG CTGCTGGAGA CTCACCGGCA CTTCTTCTTC
    AGCATGAAGG AGCGCAGGCA GGAGTCCTGC GCTGGCAGTG ACAGGAACTT TGTGATCAAC
    CAAATTGGAG ACATTCTGGT ACAACAGTTT TCAGAGGAAA ATGCAAGCAA AATGAAGAGG
    ATCTATGGAG AATTCTGCAG CCATCACAAA GAAGCCATGA GTCTCTTTAA AGAGCTGCAG
    CAGAACAAGA AGTTTCAGAA TTTTATTAAG ATCCGAAATA GCAACCTGTT GGCTCGTCGC
    CGCGGAATCC CGGAGTGCAT CCTGCTAGTT ACCCAGCGCA TCACAAAGTA CCCAGTCTTG
    GTGGAAAGGA TCCTGCAGTA CACCAAGGAA AGGACTGAAG AGCACCGGGA CCTGTGCAAA
    GCTCTCGGCT TAATTAAAGA CATGATCGCA GCCGTGGACC TGAAAGTCAG CGAATACGAG
    AAGAACCAAA AATGGCTCGA GATCCTAAAT AAAATCGAGA ACAAAACATA CACAAAGCTC
    AAAAATGGCC ACGTGTTTAG AAAGCAGGCG CTGCTGAGCC AGGAACGGGC TCTCCTGCAT
    GATGGGCTTG TCTACTGGAA AACCGCTACG GGTCGCTTCA AAGACATCCT GGCTCTGCTA
    CTAACTGACG TGCTGCTCTT CTTACAAGAA AAAGACCAGA AATACATCTT TGCAGCTGTT
    GACCAGAAGC CATCAGTGAT TTCCCTCCAG AAGCTGATTG CTAGAGAAGT TGCCAACGAG
    GAGAGAGGCA TGTTTCTGAT CAGTGCTTCG TCAGCCGGCC CCGAGATGTA CGAAATCCAC
    ACCAATTCCA AGGAGGAGCG GAATAACTGG ATGAGGCGGA TCCAGCAGGC AGTAGAAAGT
    TGTCCAGAGG AAGAAGGAGG AAGGACGAGC GAGTCGGATG AAGAGAGGCG GAAAGCTGAA
    GCTAGAGTGG CCAAGATTCA GCAATGTCAA GAAATACTCA GTAACCAAGA CCAACAGATT
    TGCACGTACT TGGAAGAAAA GCTGCATATC TACGCTGAAC TTGGGGAACT GAGTGGCTTT
    GAGGATGTCC ACCTAGAACC CCATCTGCTC ATCAAACCTG ACCCTGGAGA GCCTCCCCAG
    GCAGCGTCCC TGCTTGCAGC AGCCCTGAGA GAAGCCGAGA GTCTGCAAGT GGCTGTGAAG
    GCTTCAAAGA TGGGCGATGT AAGCCAGTCA TCCGAAGAAA GCCCTGGAGG GACTGTCCTG
    ATGGACACAC CCAGTACACA GGATGTACCA GCGTCACCAA CTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006517584.1
    CDS271..4278
    Translation

    Target ORF information:

    RefSeq Version XM_006517521.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006517521.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGGCGGC GACGAGCCCG GCTGGCCAGA GATGCAGGAG CCATGGAGTT GAGCTGCAGT 
    GAAGTGCCTC TTTATGGACA GAAGACGGTC TATGCAAAGT TTGGCAAAAA CGTGTATCTA
    CCCGAAGATG CGGAGTTTTA CTTTGTTTAT GGCGGGTCGC ATCAGAGGCA CGTGGTGATT
    GCTGACCGTG TCCAGGACAA TGTCCTCCAA TCTAGCATTC CAGGCCACTG GCTTCAGGAG
    ACGGTGACGG TATCTGTGTG CCTCTGCTCG GAAGGGTACT CCCCAGTGAC CATGGGCTCT
    GGCTCAGTGA CCTACGTGGA CAACATGGCC TGCAGGCTGG CTCGTCTGCT GGTCACTCAG
    GCTGACCGAC TCACGGCCTG CAGTCACCAG ACGCTGCTGA CCCCGTTTGC CCTGACAGTG
    GAAGCGCTGC CTGCCTTGGA TGAGGAGCTC GTGCTGGCTC TGACCCAACT GGAGCTGCCT
    TTGGGTTGGA CAGTCCTGGG AAATTCCTCA CTTGAAGTAT CTCTACACAG AGAGTCTCTC
    CTGCATCTGG CTGTGAGATG GGCCCTCCCT AAGCTATTCC ACTTCCTCTT GTGTCTCCCT
    GGAGGAGTCA AGGCCTTGAA GCTACCTAAT GAAGAGGCCA CCACACCATT AGACTTAGCT
    TTGCAAGGTG GACACTCCAC ACTGGTAGAA GACATCACAA ATTTTCAGGG TAGCCATTCC
    CCAGGCTTCT CCCGACTGCG TCTTAACGAA GAGGCCACTC TGCAGTTTGT TCATTCATCG
    GAAACATTGA CCCTGACCGT TAACCACACA GCTGAGCATC TCTTGGAAGC AGACATCAAA
    CTCTTCCGGA AATATTTCTG GGACAGAGCC TTTCTTGTCA AGGCTCTTGA GCAAGAAGCC
    AAGACTGAGA AAGCAACCAT GCCTTCTGGA GCTGCAGAAA CTGAAGAAGA GGTTAGGAAT
    TTGGAGTCTG GCAGATCACC ATCGGAGGAG GAGGAGGATG CAAAGAGCAT CAAAAGCCAG
    GTGGATGGGC CGAGTGAACA CGAAGACCAG GACCGCCTAC CTCTGGATCG CTCCTTTGAT
    GGCCTCAAAA AATCCAAGCA TGTCCCAGCG TCCCTTGCTG CAGGCCAGCT CTCTGATGTT
    CTGAACGGGG GTGATGAAGT CTACGCTAAC TGTATGGTGA TTGACCAGGT TGGCGATTTG
    GATATCAACT ATATAAACCT AGAGGGACTC TCCACCCATA CGAGTCCCGA GTCTGGGAGG
    TCCATGCTGG GGCCTCAGGC TTGCATGCAC ACCCTTCCTC CCGACACCAG TCCCTGCGGG
    CGCCCACTCA TTGAGAACAG TGAGGGGACC CTGGATGCTG CAGCTTCGCA GTCTTTCGTG
    ACACCATCCA GTTCTCGCAC TTCCAACTTG AACCTTTCTT TTGGTCTCCA TGGATTTGAA
    AAGGAGCAGA GTCATCTCAA GAAAAGGAGT TCTAGCCTGG ATGCCCTGGT TGCCGACAGT
    GAGGGGGAAG GAGGTTCTGA GCCCCCCATC TGCTATGCTG TGGGCTCTCA GAGTTCCCCA
    AGAACTGGAC TCCCCAGCGG GGATGAACTG GACTCTTTTG AGACCAACAC TGAACCAGAC
    TGTAATATCT CCAGGACCGA ATCACTCTCT CTGTCCAGTA CTCTGCATTC AAAGGAGTCC
    TTGCTTTCTG GGATCCGGTC TCGCTCCTAC TCCTGCTCAT CACCCAAAAT CTCTTCAGGA
    AAATCCCGGC TAGTGCGCGA CTTCACAGTG TGCAGTACCA GCGAAGAACA GAGATCATAT
    AGTTTCCAGG AGCCACCAGG AGAAAAAAGG ATTCAGGAAG AAGAATGGGA TGAATACGTC
    ATTCCTGCCA AATCCGAGTC TGAAAAGTAC AAAGTGAGCC GAACTTTCAG CTTCCTCATG
    AACAGGATGA CCAGCCCACG GAACAAATCT AAGATGAAAA ACAAGGACAC TAAAGAAAAA
    GAAAAAATGA ATCGACATCA GTTTGTCCCG GGAACGTTCT CCGGGGTTCT GCAGTGCTCC
    GGTTGTGATA AAACACTCCT GGGGAAAGAG TCACTCCAGT GTGCCAACTG TAAGGCAAAC
    ACACACAAGG GCTGCAAAGA TGCTGTGCCT CCGTGTACCA AGAAATTCCA AGAGAAATAC
    AACAAGAACA AACCACAGAG CATCCTTGGA AGCTCTTCAG TCAGAGACGT CCCAGCACCT
    GGGCTCTCCT TGCACCCTTC TTCCTCTATG CCCATCGGAC TGCCAGCTGG AAGGAAGGAA
    TTTGCGGCTC AGGTGCATCC ACTGTCCAGA AGTGTCCCCG GCACCACTTT GGAAAGCTTC
    AGGAGGGCAG TGACATCGTT AGAGTCTGAG GGTGACAGCT GGAGAAGCAG GTCTCATTCC
    GATGAGCTGT TCCAGTCTAT GGGCTCTTCT CCTTCCACGG AGTCATTCAT GATGGAAGAT
    GTTGTGGATT CTTCTCTGTG GATCGACCTC AGTAGTGATG CTCAGGAGTT TGAAGCAGAG
    TCTTGGAGTC TCGTGGTGGA CCCTTCCTTT TGCAGTAGGC AGGAGAAGGA TGTCATCAAA
    AGACAAGATG TCATTTTTGA GCTGATGCAG ACAGAGGTCC ACCACATCCA GACTCTGCTC
    ATCATGTCCG AGGTCTTCAG GAAAGGCATG AAGGAGGAAC TGCAGCTGGA CCACAGCACG
    GTGGACAAGA TCTTCCCCTG CTTAGATGAG CTGCTGGAGA CTCACCGGCA CTTCTTCTTC
    AGCATGAAGG AGCGCAGGCA GGAGTCCTGC GCTGGCAGTG ACAGGAACTT TGTGATCAAC
    CAAATTGGAG ACATTCTGGT ACAACAGTTT TCAGAGGAAA ATGCAAGCAA AATGAAGAGG
    ATCTATGGAG AATTCTGCAG CCATCACAAA GAAGCCATGA GTCTCTTTAA AGAGCTGCAG
    CAGAACAAGA AGTTTCAGAA TTTTATTAAG ATCCGAAATA GCAACCTGTT GGCTCGTCGC
    CGCGGAATCC CGGAGTGCAT CCTGCTAGTT ACCCAGCGCA TCACAAAGTA CCCAGTCTTG
    GTGGAAAGGA TCCTGCAGTA CACCAAGGAA AGGACTGAAG AGCACCGGGA CCTGTGCAAA
    GCTCTCGGCT TAATTAAAGA CATGATCGCA GCCGTGGACC TGAAAGTCAG CGAATACGAG
    AAGAACCAAA AATGGCTCGA GATCCTAAAT AAAATCGAGA ACAAAACATA CACAAAGCTC
    AAAAATGGCC ACGTGTTTAG AAAGCAGGCG CTGCTGAGCC AGGAACGGGC TCTCCTGCAT
    GATGGGCTTG TCTACTGGAA AACCGCTACG GGTCGCTTCA AAGACATCCT GGCTCTGCTA
    CTAACTGACG TGCTGCTCTT CTTACAAGAA AAAGACCAGA AATACATCTT TGCAGCTGTT
    GACCAGAAGC CATCAGTGAT TTCCCTCCAG AAGCTGATTG CTAGAGAAGT TGCCAACGAG
    GAGAGAGGCA TGTTTCTGAT CAGTGCTTCG TCAGCCGGCC CCGAGATGTA CGAAATCCAC
    ACCAATTCCA AGGAGGAGCG GAATAACTGG ATGAGGCGGA TCCAGCAGGC AGTAGAAAGT
    TGTCCAGAGG AAGAAGGAGG AAGGACGAGC GAGTCGGATG AAGAGAGGCG GAAAGCTGAA
    GCTAGAGTGG CCAAGATTCA GCAATGTCAA GAAATACTCA GTAACCAAGA CCAACAGATT
    TGCACGTACT TGGAAGAAAA GCTGCATATC TACGCTGAAC TTGGGGAACT GAGTGGCTTT
    GAGGATGTCC ACCTAGAACC CCATCTGCTC ATCAAACCTG ACCCTGGAGA GCCTCCCCAG
    GCAGCGTCCC TGCTTGCAGC AGCCCTGAGA GAAGCCGAGA GTCTGCAAGT GGCTGTGAAG
    GCTTCAAAGA TGGGCGATGT AAGCCAGTCA TCCGAAGAAA GCCCTGGAGG GACTGTCCTG
    ATGGACACAC CCAGTACACA GGATGTACCA GCGTCACCAA CTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.