SPON2 cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPON2
Entrez Gene ID	100689
Full Name	spondin 2, extracellular matrix protein
Synonyms	2310045I24Rik,AI504350,M-spondin,Mindin,Mspondin
General protein information	Preferred Names spondin 2, extracellular matrix protein Names spondin-2
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 5 Map Location: 5\|5 B1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006503622.2	XP_006503685.1	spondin-2 isoform X1
NM_133903.3	NP_598664.3	spondin-2 precursor
XM_006503622.1	XP_006503685.1	spondin-2 isoform X1

Pathways from BioSystems

Homologies

	SPON2	XP_001092523.1
Mus musculus (house mouse)	Spon2	NP_598664.3
Danio rerio (zebrafish)	spon2b	NP_571083.4
Drosophila melanogaster (fruit fly)	mspo	NP_725407.1
Homo sapiens (human)	SPON2	NP_036577.1
Bos taurus (cattle)	SPON2	NP_001039622.1
Canis lupus familiaris (dog)	LOC102155429	XP_005618808.1
Xenopus tropicalis (tropical clawed frog)	spon2	NP_001072560.1
Rattus norvegicus (Norway rat)	Spon2	NP_612542.1
Gallus gallus (chicken)	SPON2	XP_420847.3
Pan troglodytes (chimpanzee)	SPON2	XP_001141954.1

Gene Ontology
Bibliography

Related articles in PubMed

Uterine RAC1 via Pak1-ERM signaling directs normal luminal epithelial integrity conducive to on-time embryo implantation in mice.
Tu Z, Wang Q, Cui T, Wang J, Ran H, Bao H, Lu J, Wang B, Lydon JP, DeMayo F, Zhang S, Kong S, Wu X, Wang H
Cell death and differentiation23(1)169-81(2016 Jan)

Mindin deficiency protects the liver against ischemia/reperfusion injury.
Sun P, Zhang P, Wang PX, Zhu LH, Du Y, Tian S, Zhu X, Li H
Journal of hepatology63(5)1198-211(2015 Nov)

Identification of tool use acquisition-associated genes in the primate neocortex.
Matsunaga E, Nambu S, Oka M, Tanaka M, Taoka M, Iriki A
Development, growth & differentiation57(6)484-95(2015 Aug)

Mindin regulates vascular smooth muscle cell phenotype and prevents neointima formation.
Zhu LH, Huang L, Zhang X, Zhang P, Zhang SM, Guan H, Zhang Y, Zhu XY, Tian S, Deng K, Li H
Clinical science (London, England : 1979)129(2)129-45(2015 Jul)

Mindin/Spondin 2 inhibits hepatic steatosis, insulin resistance, and obesity via interaction with peroxisome proliferator-activated receptor ? in mice.
Zhu LH, Wang A, Luo P, Wang X, Jiang DS, Deng W, Zhang X, Wang T, Liu Y, Gao L, Zhang S, Zhang X, Zhang J, Li H
Journal of hepatology60(5)1046-54(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mindin is a novel modulator of hepatic I/R injury through regulating inflammatory responses, as well as hepatocyte apoptosis and proliferation via inactivation of the Akt signaling pathway.
Title: Mindin deficiency protects the liver against ischemia/reperfusion injury.

Mindin protects against vascular hyperplasia by suppression of abnormal VSMC proliferation, migration and phenotypic switching in an AKT-dependent manner.
Title: Mindin regulates vascular smooth muscle cell phenotype and prevents neointima formation.

Spondin 2 regulates hepatic lipid metabolism and alleviates hepatic steatosis, obesity, inflammation, and insulin resistance via its interaction with PPARalpha.
Title: Mindin/Spondin 2 inhibits hepatic steatosis, insulin resistance, and obesity via interaction with peroxisome proliferator-activated receptor ? in mice.

The data of this study demonstrated that mindin is a critical mediator of ischemic brain injury in an experimental stroke model.
Title: Mindin is a critical mediator of ischemic brain injury in an experimental stroke model.

Data show that mindin, as an intrinsic cardioprotective factor, prevents maladaptive remodelling and the transition to heart failure by blocking AKT/GSK3beta signalling.
Title: Disruption of mindin exacerbates cardiac hypertrophy and fibrosis.

The following SPON2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPON2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu10335	NM_133903.3 Latest version!	Mus musculus spondin 2, extracellular matrix protein (Spon2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu10335	XM_006503622.2 Latest version!	Mus musculus spondin 2, extracellular matrix protein (Spon2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
View Old Accession Versions >>
OMu10335	XM_006503622.1	Mus musculus spondin 2, extracellular matrix protein (Spon2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPON2 ( NM_133903.3 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_598664.3 Mus musculus spondin 2, extracellular matrix protein (Spon2), mRNA.

Price & Availability↑

CloneID	OMu10335
Clone ID Related Accession (Same CDS sequence)	XM_006503622.2 , NM_133903.3 , XM_006503622.1
Accession Version	NM_133903.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-07
Organism	Mus musculus(house mouse)
Product	spondin-2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB848417.1, AK028987.1 and AC115070.9. On Jul 31, 2009 this sequence version replaced NM_133903.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK028987.1, BC017616.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAAACG TGAGTCTTGC CCTGGGCAGA GCTCTTTGGG TCTTCCTCCT GGCCATGATA 
GGCTCCACCA CGAGCCAGCC GCTGGGGGGA GAATCGGTTT GTACAGCCCG GCCTCTGGCT 
AGATACAGCA TCACTTTCAT TGGCAAGTGG AGCCAGACAG CATTTCCCAA GCAGTACCCC 
CTGTTCCGGC CCCCTGCACA GTGGTCCTCT CTGCTGGGGG CAGCTCACAG CTCTGACTAC 
AGCATGTGGC GGAAGAATGA GTATGTCAGC AATGGGCTGA GGGACTTTGC TGAGCGTGGT 
GAGGCCTGGG CACTGATGAA GGAGATCGAA GCTGCAGGAG AGAAACTTCA GAGTGTGCAC 
GCAGTGTTCT CAGCTCCTGC CATCCCTAGC GGTACCGGGC AGACTTCCAC AGAGTTAGAG 
GTGCACCCTA GGCACTCGCT GGTGTCCTTC GTGGTACGCA TTGTCCCCAG CCCTGACTGG 
TTTGTGGGCA TCGACAGTCT GGACCTGTGT GAGGGAGGCC GCTGGAAGGA GCAGGTGGTC 
CTGGATCTTT ATCCACACGA TGCAGGGACC GACAGTGGTT TCACCTTCTC CTCCCCTAAC 
TTTGCAACTA TCCCACAAGA CACAGTGACT GAGATAACAG CCTCATCGCC CAGCCACCCA 
GCAAATTCAT TCTACTACCC ACGCCTCAAG TCCTTACCTC CCATCGCCAA AGTGACCTTC 
GTGCGGCTAC AGCAGAGTCC CAGGGCCTTT GCCCCACCTT CCCTGGACCT GGCCAGCCGA 
GGCAACGAAA TCGTTGACAG CCTTTCAGTT CCAGAGACAC CGCTGGACTG TGAGGTTTCC 
CTGTGGTCAT CCTGGGGACT GTGTGGAGGA CCATGTGGAA AGTTGGGAGC CAAGAGCAGA 
ACTCGCTATG TCCGTGTTCA GCCTGCTAAC AATGGGACTC CCTGTCCCGA GCTTGAAGAA 
GAGGCCGAGT GTGCCCCAGA TAACTGCGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598664.3
CDS	295..1287
Translation	MENVSLALGRALWVFLLAMIGSTTSQPLGGESVCTARPLARYSITFIGKWSQTAFPKQYPLFRPPAQWSSLLGAAHSSDYSMWRKNEYVSNGLRDFAERGEAWALMKEIEAAGEKLQSVHAVFSAPAIPSGTGQTSTELEVHPRHSLVSFVVRIVPSPDWFVGIDSLDLCEGGRWKEQVVLDLYPHDAGTDSGFTFSSPNFATIPQDTVTEITASSPSHPANSFYYPRLKSLPPIAKVTFVRLQQSPRAFAPPSLDLASRGNEIVDSLSVPETPLDCEVSLWSSWGLCGGPCGKLGAKSRTRYVRVQPANNGTPCPELEEEAECAPDNCV

Target ORF information:

RefSeq Version	NM_133903.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus spondin 2, extracellular matrix protein (Spon2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133903.3

ORF Insert Sequence:

ATGGAAAACG TGAGTCTTGC CCTGGGCAGA GCTCTTTGGG TCTTCCTCCT GGCCATGATA 
GGCTCCACCA CGAGCCAGCC GCTGGGGGGA GAATCGGTTT GTACAGCCCG GCCTCTGGCT 
AGATACAGCA TCACTTTCAT TGGCAAGTGG AGCCAGACAG CATTTCCCAA GCAGTACCCC 
CTGTTCCGGC CCCCTGCACA GTGGTCCTCT CTGCTGGGGG CAGCTCACAG CTCTGACTAC 
AGCATGTGGC GGAAGAATGA GTATGTCAGC AATGGGCTGA GGGACTTTGC TGAGCGTGGT 
GAGGCCTGGG CACTGATGAA GGAGATCGAA GCTGCAGGAG AGAAACTTCA GAGTGTGCAC 
GCAGTGTTCT CAGCTCCTGC CATCCCTAGC GGTACCGGGC AGACTTCCAC AGAGTTAGAG 
GTGCACCCTA GGCACTCGCT GGTGTCCTTC GTGGTACGCA TTGTCCCCAG CCCTGACTGG 
TTTGTGGGCA TCGACAGTCT GGACCTGTGT GAGGGAGGCC GCTGGAAGGA GCAGGTGGTC 
CTGGATCTTT ATCCACACGA TGCAGGGACC GACAGTGGTT TCACCTTCTC CTCCCCTAAC 
TTTGCAACTA TCCCACAAGA CACAGTGACT GAGATAACAG CCTCATCGCC CAGCCACCCA 
GCAAATTCAT TCTACTACCC ACGCCTCAAG TCCTTACCTC CCATCGCCAA AGTGACCTTC 
GTGCGGCTAC AGCAGAGTCC CAGGGCCTTT GCCCCACCTT CCCTGGACCT GGCCAGCCGA 
GGCAACGAAA TCGTTGACAG CCTTTCAGTT CCAGAGACAC CGCTGGACTG TGAGGTTTCC 
CTGTGGTCAT CCTGGGGACT GTGTGGAGGA CCATGTGGAA AGTTGGGAGC CAAGAGCAGA 
ACTCGCTATG TCCGTGTTCA GCCTGCTAAC AATGGGACTC CCTGTCCCGA GCTTGAAGAA 
GAGGCCGAGT GTGCCCCAGA TAACTGCGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPON2 ( XM_006503622.2 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006503685.1 Mus musculus spondin 2, extracellular matrix protein (Spon2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu10335
Clone ID Related Accession (Same CDS sequence)	XM_006503622.2 , NM_133903.3 , XM_006503622.1
Accession Version	XM_006503622.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	spondin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006503622.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAACG TGAGTCTTGC CCTGGGCAGA GCTCTTTGGG TCTTCCTCCT GGCCATGATA 
GGCTCCACCA CGAGCCAGCC GCTGGGGGGA GAATCGGTTT GTACAGCCCG GCCTCTGGCT 
AGATACAGCA TCACTTTCAT TGGCAAGTGG AGCCAGACAG CATTTCCCAA GCAGTACCCC 
CTGTTCCGGC CCCCTGCACA GTGGTCCTCT CTGCTGGGGG CAGCTCACAG CTCTGACTAC 
AGCATGTGGC GGAAGAATGA GTATGTCAGC AATGGGCTGA GGGACTTTGC TGAGCGTGGT 
GAGGCCTGGG CACTGATGAA GGAGATCGAA GCTGCAGGAG AGAAACTTCA GAGTGTGCAC 
GCAGTGTTCT CAGCTCCTGC CATCCCTAGC GGTACCGGGC AGACTTCCAC AGAGTTAGAG 
GTGCACCCTA GGCACTCGCT GGTGTCCTTC GTGGTACGCA TTGTCCCCAG CCCTGACTGG 
TTTGTGGGCA TCGACAGTCT GGACCTGTGT GAGGGAGGCC GCTGGAAGGA GCAGGTGGTC 
CTGGATCTTT ATCCACACGA TGCAGGGACC GACAGTGGTT TCACCTTCTC CTCCCCTAAC 
TTTGCAACTA TCCCACAAGA CACAGTGACT GAGATAACAG CCTCATCGCC CAGCCACCCA 
GCAAATTCAT TCTACTACCC ACGCCTCAAG TCCTTACCTC CCATCGCCAA AGTGACCTTC 
GTGCGGCTAC AGCAGAGTCC CAGGGCCTTT GCCCCACCTT CCCTGGACCT GGCCAGCCGA 
GGCAACGAAA TCGTTGACAG CCTTTCAGTT CCAGAGACAC CGCTGGACTG TGAGGTTTCC 
CTGTGGTCAT CCTGGGGACT GTGTGGAGGA CCATGTGGAA AGTTGGGAGC CAAGAGCAGA 
ACTCGCTATG TCCGTGTTCA GCCTGCTAAC AATGGGACTC CCTGTCCCGA GCTTGAAGAA 
GAGGCCGAGT GTGCCCCAGA TAACTGCGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503685.1
CDS	399..1391
Translation	MENVSLALGRALWVFLLAMIGSTTSQPLGGESVCTARPLARYSITFIGKWSQTAFPKQYPLFRPPAQWSSLLGAAHSSDYSMWRKNEYVSNGLRDFAERGEAWALMKEIEAAGEKLQSVHAVFSAPAIPSGTGQTSTELEVHPRHSLVSFVVRIVPSPDWFVGIDSLDLCEGGRWKEQVVLDLYPHDAGTDSGFTFSSPNFATIPQDTVTEITASSPSHPANSFYYPRLKSLPPIAKVTFVRLQQSPRAFAPPSLDLASRGNEIVDSLSVPETPLDCEVSLWSSWGLCGGPCGKLGAKSRTRYVRVQPANNGTPCPELEEEAECAPDNCV

Target ORF information:

RefSeq Version	XM_006503622.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus spondin 2, extracellular matrix protein (Spon2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503622.2

ORF Insert Sequence:

ATGGAAAACG TGAGTCTTGC CCTGGGCAGA GCTCTTTGGG TCTTCCTCCT GGCCATGATA 
GGCTCCACCA CGAGCCAGCC GCTGGGGGGA GAATCGGTTT GTACAGCCCG GCCTCTGGCT 
AGATACAGCA TCACTTTCAT TGGCAAGTGG AGCCAGACAG CATTTCCCAA GCAGTACCCC 
CTGTTCCGGC CCCCTGCACA GTGGTCCTCT CTGCTGGGGG CAGCTCACAG CTCTGACTAC 
AGCATGTGGC GGAAGAATGA GTATGTCAGC AATGGGCTGA GGGACTTTGC TGAGCGTGGT 
GAGGCCTGGG CACTGATGAA GGAGATCGAA GCTGCAGGAG AGAAACTTCA GAGTGTGCAC 
GCAGTGTTCT CAGCTCCTGC CATCCCTAGC GGTACCGGGC AGACTTCCAC AGAGTTAGAG 
GTGCACCCTA GGCACTCGCT GGTGTCCTTC GTGGTACGCA TTGTCCCCAG CCCTGACTGG 
TTTGTGGGCA TCGACAGTCT GGACCTGTGT GAGGGAGGCC GCTGGAAGGA GCAGGTGGTC 
CTGGATCTTT ATCCACACGA TGCAGGGACC GACAGTGGTT TCACCTTCTC CTCCCCTAAC 
TTTGCAACTA TCCCACAAGA CACAGTGACT GAGATAACAG CCTCATCGCC CAGCCACCCA 
GCAAATTCAT TCTACTACCC ACGCCTCAAG TCCTTACCTC CCATCGCCAA AGTGACCTTC 
GTGCGGCTAC AGCAGAGTCC CAGGGCCTTT GCCCCACCTT CCCTGGACCT GGCCAGCCGA 
GGCAACGAAA TCGTTGACAG CCTTTCAGTT CCAGAGACAC CGCTGGACTG TGAGGTTTCC 
CTGTGGTCAT CCTGGGGACT GTGTGGAGGA CCATGTGGAA AGTTGGGAGC CAAGAGCAGA 
ACTCGCTATG TCCGTGTTCA GCCTGCTAAC AATGGGACTC CCTGTCCCGA GCTTGAAGAA 
GAGGCCGAGT GTGCCCCAGA TAACTGCGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPON2 ( XM_006503622.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006503685.1 Mus musculus spondin 2, extracellular matrix protein (Spon2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu10335
Clone ID Related Accession (Same CDS sequence)	XM_006503622.2 , NM_133903.3 , XM_006503622.1
Accession Version	XM_006503622.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	spondin-2 isoform X1
Comment

ATGGAAAACG TGAGTCTTGC CCTGGGCAGA GCTCTTTGGG TCTTCCTCCT GGCCATGATA 
GGCTCCACCA CGAGCCAGCC GCTGGGGGGA GAATCGGTTT GTACAGCCCG GCCTCTGGCT 
AGATACAGCA TCACTTTCAT TGGCAAGTGG AGCCAGACAG CATTTCCCAA GCAGTACCCC 
CTGTTCCGGC CCCCTGCACA GTGGTCCTCT CTGCTGGGGG CAGCTCACAG CTCTGACTAC 
AGCATGTGGC GGAAGAATGA GTATGTCAGC AATGGGCTGA GGGACTTTGC TGAGCGTGGT 
GAGGCCTGGG CACTGATGAA GGAGATCGAA GCTGCAGGAG AGAAACTTCA GAGTGTGCAC 
GCAGTGTTCT CAGCTCCTGC CATCCCTAGC GGTACCGGGC AGACTTCCAC AGAGTTAGAG 
GTGCACCCTA GGCACTCGCT GGTGTCCTTC GTGGTACGCA TTGTCCCCAG CCCTGACTGG 
TTTGTGGGCA TCGACAGTCT GGACCTGTGT GAGGGAGGCC GCTGGAAGGA GCAGGTGGTC 
CTGGATCTTT ATCCACACGA TGCAGGGACC GACAGTGGTT TCACCTTCTC CTCCCCTAAC 
TTTGCAACTA TCCCACAAGA CACAGTGACT GAGATAACAG CCTCATCGCC CAGCCACCCA 
GCAAATTCAT TCTACTACCC ACGCCTCAAG TCCTTACCTC CCATCGCCAA AGTGACCTTC 
GTGCGGCTAC AGCAGAGTCC CAGGGCCTTT GCCCCACCTT CCCTGGACCT GGCCAGCCGA 
GGCAACGAAA TCGTTGACAG CCTTTCAGTT CCAGAGACAC CGCTGGACTG TGAGGTTTCC 
CTGTGGTCAT CCTGGGGACT GTGTGGAGGA CCATGTGGAA AGTTGGGAGC CAAGAGCAGA 
ACTCGCTATG TCCGTGTTCA GCCTGCTAAC AATGGGACTC CCTGTCCCGA GCTTGAAGAA 
GAGGCCGAGT GTGCCCCAGA TAACTGCGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503685.1
Translation	MENVSLALGRALWVFLLAMIGSTTSQPLGGESVCTARPLARYSITFIGKWSQTAFPKQYPLFRPPAQWSSLLGAAHSSDYSMWRKNEYVSNGLRDFAERGEAWALMKEIEAAGEKLQSVHAVFSAPAIPSGTGQTSTELEVHPRHSLVSFVVRIVPSPDWFVGIDSLDLCEGGRWKEQVVLDLYPHDAGTDSGFTFSSPNFATIPQDTVTEITASSPSHPANSFYYPRLKSLPPIAKVTFVRLQQSPRAFAPPSLDLASRGNEIVDSLSVPETPLDCEVSLWSSWGLCGGPCGKLGAKSRTRYVRVQPANNGTPCPELEEEAECAPDNCV

Target ORF information:

RefSeq Version	XM_006503622.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus spondin 2, extracellular matrix protein (Spon2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503622.1

ORF Insert Sequence:

ATGGAAAACG TGAGTCTTGC CCTGGGCAGA GCTCTTTGGG TCTTCCTCCT GGCCATGATA 
GGCTCCACCA CGAGCCAGCC GCTGGGGGGA GAATCGGTTT GTACAGCCCG GCCTCTGGCT 
AGATACAGCA TCACTTTCAT TGGCAAGTGG AGCCAGACAG CATTTCCCAA GCAGTACCCC 
CTGTTCCGGC CCCCTGCACA GTGGTCCTCT CTGCTGGGGG CAGCTCACAG CTCTGACTAC 
AGCATGTGGC GGAAGAATGA GTATGTCAGC AATGGGCTGA GGGACTTTGC TGAGCGTGGT 
GAGGCCTGGG CACTGATGAA GGAGATCGAA GCTGCAGGAG AGAAACTTCA GAGTGTGCAC 
GCAGTGTTCT CAGCTCCTGC CATCCCTAGC GGTACCGGGC AGACTTCCAC AGAGTTAGAG 
GTGCACCCTA GGCACTCGCT GGTGTCCTTC GTGGTACGCA TTGTCCCCAG CCCTGACTGG 
TTTGTGGGCA TCGACAGTCT GGACCTGTGT GAGGGAGGCC GCTGGAAGGA GCAGGTGGTC 
CTGGATCTTT ATCCACACGA TGCAGGGACC GACAGTGGTT TCACCTTCTC CTCCCCTAAC 
TTTGCAACTA TCCCACAAGA CACAGTGACT GAGATAACAG CCTCATCGCC CAGCCACCCA 
GCAAATTCAT TCTACTACCC ACGCCTCAAG TCCTTACCTC CCATCGCCAA AGTGACCTTC 
GTGCGGCTAC AGCAGAGTCC CAGGGCCTTT GCCCCACCTT CCCTGGACCT GGCCAGCCGA 
GGCAACGAAA TCGTTGACAG CCTTTCAGTT CCAGAGACAC CGCTGGACTG TGAGGTTTCC 
CTGTGGTCAT CCTGGGGACT GTGTGGAGGA CCATGTGGAA AGTTGGGAGC CAAGAGCAGA 
ACTCGCTATG TCCGTGTTCA GCCTGCTAAC AATGGGACTC CCTGTCCCGA GCTTGAAGAA 
GAGGCCGAGT GTGCCCCAGA TAACTGCGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Uterine RAC1 via Pak1-ERM signaling directs normal luminal epithelial integrity conducive to on-time embryo implantation in mice.
Cell death and differentiation23(1)169-81(2016 Jan)
Tu Z,Wang Q,Cui T,Wang J,Ran H,Bao H,Lu J,Wang B,Lydon JP,DeMayo F,Zhang S,Kong S,Wu X,Wang H

Mindin deficiency protects the liver against ischemia/reperfusion injury.
Journal of hepatology63(5)1198-211(2015 Nov)
Sun P,Zhang P,Wang PX,Zhu LH,Du Y,Tian S,Zhu X,Li H

Identification of tool use acquisition-associated genes in the primate neocortex.
Development, growth & differentiation57(6)484-95(2015 Aug)
Matsunaga E,Nambu S,Oka M,Tanaka M,Taoka M,Iriki A

Mindin regulates vascular smooth muscle cell phenotype and prevents neointima formation.
Clinical science (London, England : 1979)129(2)129-45(2015 Jul)
Zhu LH,Huang L,Zhang X,Zhang P,Zhang SM,Guan H,Zhang Y,Zhu XY,Tian S,Deng K,Li H

Mindin/Spondin 2 inhibits hepatic steatosis, insulin resistance, and obesity via interaction with peroxisome proliferator-activated receptor ? in mice.
Journal of hepatology60(5)1046-54(2014 May)
Zhu LH,Wang A,Luo P,Wang X,Jiang DS,Deng W,Zhang X,Wang T,Liu Y,Gao L,Zhang S,Zhang X,Zhang J,Li H

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SPON2 cDNA ORF clone, Mus musculus(House mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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