SPON2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPON2
Entrez Gene ID	10417
Full Name	spondin 2
Synonyms	DIL-1,DIL1,M-SPONDIN,MINDIN
General protein information	Preferred Names spondin 2 Names spondin-2 differentially expressed in cancerous and non-cancerous lung cells 1 spondin 2, extracellular matrix protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4p16.3
Disorder MIM:	605918

mRNA and Protein(s)

mRNA	Protein	Name
NM_012445.4	NP_036577.2	spondin-2 precursor
NM_012445.3	NP_036577.1	spondin-2 precursor
NM_001199021.1	NP_001185950.1	spondin-2 precursor
NM_001128325.2	NP_001121797.1	spondin-2 precursor

Pathways from BioSystems

Homologies

	SPON2	XP_001092523.1
Mus musculus (house mouse)	Spon2	NP_598664.3
Danio rerio (zebrafish)	spon2b	NP_571083.4
Drosophila melanogaster (fruit fly)	mspo	NP_725407.1
Homo sapiens (human)	SPON2	NP_036577.1
Bos taurus (cattle)	SPON2	NP_001039622.1
Canis lupus familiaris (dog)	LOC102155429	XP_005618808.1
Xenopus tropicalis (tropical clawed frog)	spon2	NP_001072560.1
Rattus norvegicus (Norway rat)	Spon2	NP_612542.1
Gallus gallus (chicken)	SPON2	XP_420847.3
Pan troglodytes (chimpanzee)	SPON2	XP_001141954.1

Gene Ontology
Bibliography

Related articles in PubMed

The extracellular matrix protein mindin attenuates colon cancer progression by blocking angiogenesis via Egr-1-mediated regulation.
Wang LF, Liu YS, Yang B, Li P, Cheng XS, Xiao CX, Liu JJ, Li S, Ren JL, Guleng B
Oncogene(2017 Oct)

Elevated spondin-2 expression correlates with progression and prognosis in gastric cancer.
Jin C, Lin JR, Ma L, Song Y, Shi YX, Jiang P, Dong Y, Li XS
Oncotarget8(6)10416-10424(2017 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SPON2 is a transcriptional target of the metastasis gene MACC1. SPON2 induces cell motility in vitro and CRC metastasis in mice. In patients, SPON2 serves as prognostic indicator for CRC metastasis and survival, and might represent a promising target for therapeutic approaches.
Title: SPON2, a newly identified target gene of MACC1, drives colorectal cancer metastasis in mice and is prognostic for colorectal cancer patient survival.

Mindin protects against vascular hyperplasia by suppression of abnormal VSMC proliferation, migration and phenotypic switching in an AKT-dependent manner.
Title: Mindin regulates vascular smooth muscle cell phenotype and prevents neointima formation.

Data suggest that spondin-2 could be an independent diagnostic and prognostic biomarker of colon cancer.
Title: Upregulation of spondin-2 predicts poor survival of colorectal carcinoma patients.

Using tissue microarray by immunohistostaining, we found SPON2 to be over-expressed in prostate cancer (PCa). SPON2 staining was more intense in Gleason score sum 7-8 and in PCa patients with metastasis.
Title: Spondin-2 (SPON2), a more prostate-cancer-specific diagnostic biomarker.

mindin serves as a novel mediator that protects against cardiac hypertrophy and the transition to heart failure by blocking AKT/GSK3beta and TGF-beta1-Smad signalling.
Title: Cardiac-specific mindin overexpression attenuates cardiac hypertrophy via blocking AKT/GSK3? and TGF-?1-Smad signalling.

The following SPON2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPON2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu07709	NM_012445.3 Latest version!	Homo sapiens spondin 2 (SPON2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu07709	NM_001199021.1 Latest version!	Homo sapiens spondin 2 (SPON2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu07709	NM_001128325.2 Latest version!	Homo sapiens spondin 2 (SPON2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu107292	NM_012445.4 Latest version!	Homo sapiens spondin 2 (SPON2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPON2 ( NM_012445.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_036577.1 Homo sapiens spondin 2 (SPON2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu07709
Clone ID Related Accession (Same CDS sequence)	NM_012445.3 , NM_001199021.1 , NM_001128325.2
Accession Version	NM_012445.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-10
Organism	Homo sapiens(human)
Product	spondin-2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA375259.1, AB027466.1, BC002707.2 and AA864573.1. On Nov 18, 2010 this sequence version replaced NM_012445.2. Transcript Variant: This variant (1) encodes the same protein as variants 2 and 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY358948.1, BC036341.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAAACC CCAGCCCGGC CGCCGCCCTG GGCAAGGCCC TCTGCGCTCT CCTCCTGGCC 
ACTCTCGGCG CCGCCGGCCA GCCTCTTGGG GGAGAGTCCA TCTGTTCCGC CAGAGCCCCG 
GCCAAATACA GCATCACCTT CACGGGCAAG TGGAGCCAGA CGGCCTTCCC CAAGCAGTAC 
CCCCTGTTCC GCCCCCCTGC GCAGTGGTCT TCGCTGCTGG GGGCCGCGCA TAGCTCCGAC 
TACAGCATGT GGAGGAAGAA CCAGTACGTC AGTAACGGGC TGCGCGACTT TGCGGAGCGC 
GGCGAGGCCT GGGCGCTGAT GAAGGAGATC GAGGCGGCGG GGGAGGCGCT GCAGAGCGTG 
CACGCGGTGT TTTCGGCGCC CGCCGTCCCC AGCGGCACCG GGCAGACGTC GGCGGAGCTG 
GAGGTGCAGC GCAGGCACTC GCTGGTCTCG TTTGTGGTGC GCATCGTGCC CAGCCCCGAC 
TGGTTCGTGG GCGTGGACAG CCTGGACCTG TGCGACGGGG ACCGTTGGCG GGAACAGGCG 
GCGCTGGACC TGTACCCCTA CGACGCCGGG ACGGACAGCG GCTTCACCTT CTCCTCCCCC 
AACTTCGCCA CCATCCCGCA GGACACGGTG ACCGAGATAA CGTCCTCCTC TCCCAGCCAC 
CCGGCCAACT CCTTCTACTA CCCGCGGCTG AAGGCCCTGC CTCCCATCGC CAGGGTGACA 
CTGGTGCGGC TGCGACAGAG CCCCAGGGCC TTCATCCCTC CCGCCCCAGT CCTGCCCAGC 
AGGGACAATG AGATTGTAGA CAGCGCCTCA GTTCCAGAAA CGCCGCTGGA CTGCGAGGTC 
TCCCTGTGGT CGTCCTGGGG ACTGTGCGGA GGCCACTGTG GGAGGCTCGG GACCAAGAGC 
AGGACTCGCT ACGTCCGGGT CCAGCCCGCC AACAACGGGA GCCCCTGCCC CGAGCTCGAA 
GAAGAGGCTG AGTGCGTCCC TGATAACTGC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036577.1
CDS	330..1325
Translation	MENPSPAAALGKALCALLLATLGAAGQPLGGESICSARAPAKYSITFTGKWSQTAFPKQYPLFRPPAQWSSLLGAAHSSDYSMWRKNQYVSNGLRDFAERGEAWALMKEIEAAGEALQSVHAVFSAPAVPSGTGQTSAELEVQRRHSLVSFVVRIVPSPDWFVGVDSLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEITSSSPSHPANSFYYPRLKALPPIARVTLVRLRQSPRAFIPPAPVLPSRDNEIVDSASVPETPLDCEVSLWSSWGLCGGHCGRLGTKSRTRYVRVQPANNGSPCPELEEEAECVPDNCV

Target ORF information:

RefSeq Version	NM_012445.3
Organism	Homo sapiens(human)
Definition	Homo sapiens spondin 2 (SPON2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012445.3

ORF Insert Sequence:

ATGGAAAACC CCAGCCCGGC CGCCGCCCTG GGCAAGGCCC TCTGCGCTCT CCTCCTGGCC 
ACTCTCGGCG CCGCCGGCCA GCCTCTTGGG GGAGAGTCCA TCTGTTCCGC CAGAGCCCCG 
GCCAAATACA GCATCACCTT CACGGGCAAG TGGAGCCAGA CGGCCTTCCC CAAGCAGTAC 
CCCCTGTTCC GCCCCCCTGC GCAGTGGTCT TCGCTGCTGG GGGCCGCGCA TAGCTCCGAC 
TACAGCATGT GGAGGAAGAA CCAGTACGTC AGTAACGGGC TGCGCGACTT TGCGGAGCGC 
GGCGAGGCCT GGGCGCTGAT GAAGGAGATC GAGGCGGCGG GGGAGGCGCT GCAGAGCGTG 
CACGCGGTGT TTTCGGCGCC CGCCGTCCCC AGCGGCACCG GGCAGACGTC GGCGGAGCTG 
GAGGTGCAGC GCAGGCACTC GCTGGTCTCG TTTGTGGTGC GCATCGTGCC CAGCCCCGAC 
TGGTTCGTGG GCGTGGACAG CCTGGACCTG TGCGACGGGG ACCGTTGGCG GGAACAGGCG 
GCGCTGGACC TGTACCCCTA CGACGCCGGG ACGGACAGCG GCTTCACCTT CTCCTCCCCC 
AACTTCGCCA CCATCCCGCA GGACACGGTG ACCGAGATAA CGTCCTCCTC TCCCAGCCAC 
CCGGCCAACT CCTTCTACTA CCCGCGGCTG AAGGCCCTGC CTCCCATCGC CAGGGTGACA 
CTGGTGCGGC TGCGACAGAG CCCCAGGGCC TTCATCCCTC CCGCCCCAGT CCTGCCCAGC 
AGGGACAATG AGATTGTAGA CAGCGCCTCA GTTCCAGAAA CGCCGCTGGA CTGCGAGGTC 
TCCCTGTGGT CGTCCTGGGG ACTGTGCGGA GGCCACTGTG GGAGGCTCGG GACCAAGAGC 
AGGACTCGCT ACGTCCGGGT CCAGCCCGCC AACAACGGGA GCCCCTGCCC CGAGCTCGAA 
GAAGAGGCTG AGTGCGTCCC TGATAACTGC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPON2 ( NM_001199021.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001185950.1 Homo sapiens spondin 2 (SPON2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu07709
Clone ID Related Accession (Same CDS sequence)	NM_012445.3 , NM_001199021.1 , NM_001128325.2
Accession Version	NM_001199021.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-17
Organism	Homo sapiens(human)
Product	spondin-2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092535.4, DA273423.1, AB027466.1, BC002707.2 and AA864573.1. On Nov 18, 2010 this sequence version replaced NR_033182.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. It also represents a read-through transcript because it shares its first exon with that of the upstream, non-coding gene (GeneID:100130872). Variants 1, 2 and 3 all encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AY358948.1, ERR279873.1867.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## readthrough transcript :: includes exons from GeneID 100130872 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAAAACC CCAGCCCGGC CGCCGCCCTG GGCAAGGCCC TCTGCGCTCT CCTCCTGGCC 
ACTCTCGGCG CCGCCGGCCA GCCTCTTGGG GGAGAGTCCA TCTGTTCCGC CAGAGCCCCG 
GCCAAATACA GCATCACCTT CACGGGCAAG TGGAGCCAGA CGGCCTTCCC CAAGCAGTAC 
CCCCTGTTCC GCCCCCCTGC GCAGTGGTCT TCGCTGCTGG GGGCCGCGCA TAGCTCCGAC 
TACAGCATGT GGAGGAAGAA CCAGTACGTC AGTAACGGGC TGCGCGACTT TGCGGAGCGC 
GGCGAGGCCT GGGCGCTGAT GAAGGAGATC GAGGCGGCGG GGGAGGCGCT GCAGAGCGTG 
CACGCGGTGT TTTCGGCGCC CGCCGTCCCC AGCGGCACCG GGCAGACGTC GGCGGAGCTG 
GAGGTGCAGC GCAGGCACTC GCTGGTCTCG TTTGTGGTGC GCATCGTGCC CAGCCCCGAC 
TGGTTCGTGG GCGTGGACAG CCTGGACCTG TGCGACGGGG ACCGTTGGCG GGAACAGGCG 
GCGCTGGACC TGTACCCCTA CGACGCCGGG ACGGACAGCG GCTTCACCTT CTCCTCCCCC 
AACTTCGCCA CCATCCCGCA GGACACGGTG ACCGAGATAA CGTCCTCCTC TCCCAGCCAC 
CCGGCCAACT CCTTCTACTA CCCGCGGCTG AAGGCCCTGC CTCCCATCGC CAGGGTGACA 
CTGGTGCGGC TGCGACAGAG CCCCAGGGCC TTCATCCCTC CCGCCCCAGT CCTGCCCAGC 
AGGGACAATG AGATTGTAGA CAGCGCCTCA GTTCCAGAAA CGCCGCTGGA CTGCGAGGTC 
TCCCTGTGGT CGTCCTGGGG ACTGTGCGGA GGCCACTGTG GGAGGCTCGG GACCAAGAGC 
AGGACTCGCT ACGTCCGGGT CCAGCCCGCC AACAACGGGA GCCCCTGCCC CGAGCTCGAA 
GAAGAGGCTG AGTGCGTCCC TGATAACTGC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185950.1
CDS	626..1621
Translation	MENPSPAAALGKALCALLLATLGAAGQPLGGESICSARAPAKYSITFTGKWSQTAFPKQYPLFRPPAQWSSLLGAAHSSDYSMWRKNQYVSNGLRDFAERGEAWALMKEIEAAGEALQSVHAVFSAPAVPSGTGQTSAELEVQRRHSLVSFVVRIVPSPDWFVGVDSLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEITSSSPSHPANSFYYPRLKALPPIARVTLVRLRQSPRAFIPPAPVLPSRDNEIVDSASVPETPLDCEVSLWSSWGLCGGHCGRLGTKSRTRYVRVQPANNGSPCPELEEEAECVPDNCV

Target ORF information:

RefSeq Version	NM_001199021.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spondin 2 (SPON2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199021.1

ORF Insert Sequence:

ATGGAAAACC CCAGCCCGGC CGCCGCCCTG GGCAAGGCCC TCTGCGCTCT CCTCCTGGCC 
ACTCTCGGCG CCGCCGGCCA GCCTCTTGGG GGAGAGTCCA TCTGTTCCGC CAGAGCCCCG 
GCCAAATACA GCATCACCTT CACGGGCAAG TGGAGCCAGA CGGCCTTCCC CAAGCAGTAC 
CCCCTGTTCC GCCCCCCTGC GCAGTGGTCT TCGCTGCTGG GGGCCGCGCA TAGCTCCGAC 
TACAGCATGT GGAGGAAGAA CCAGTACGTC AGTAACGGGC TGCGCGACTT TGCGGAGCGC 
GGCGAGGCCT GGGCGCTGAT GAAGGAGATC GAGGCGGCGG GGGAGGCGCT GCAGAGCGTG 
CACGCGGTGT TTTCGGCGCC CGCCGTCCCC AGCGGCACCG GGCAGACGTC GGCGGAGCTG 
GAGGTGCAGC GCAGGCACTC GCTGGTCTCG TTTGTGGTGC GCATCGTGCC CAGCCCCGAC 
TGGTTCGTGG GCGTGGACAG CCTGGACCTG TGCGACGGGG ACCGTTGGCG GGAACAGGCG 
GCGCTGGACC TGTACCCCTA CGACGCCGGG ACGGACAGCG GCTTCACCTT CTCCTCCCCC 
AACTTCGCCA CCATCCCGCA GGACACGGTG ACCGAGATAA CGTCCTCCTC TCCCAGCCAC 
CCGGCCAACT CCTTCTACTA CCCGCGGCTG AAGGCCCTGC CTCCCATCGC CAGGGTGACA 
CTGGTGCGGC TGCGACAGAG CCCCAGGGCC TTCATCCCTC CCGCCCCAGT CCTGCCCAGC 
AGGGACAATG AGATTGTAGA CAGCGCCTCA GTTCCAGAAA CGCCGCTGGA CTGCGAGGTC 
TCCCTGTGGT CGTCCTGGGG ACTGTGCGGA GGCCACTGTG GGAGGCTCGG GACCAAGAGC 
AGGACTCGCT ACGTCCGGGT CCAGCCCGCC AACAACGGGA GCCCCTGCCC CGAGCTCGAA 
GAAGAGGCTG AGTGCGTCCC TGATAACTGC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPON2 ( NM_001128325.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001121797.1 Homo sapiens spondin 2 (SPON2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu07709
Clone ID Related Accession (Same CDS sequence)	NM_012445.3 , NM_001199021.1 , NM_001128325.2
Accession Version	NM_001128325.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-17
Organism	Homo sapiens(human)
Product	spondin-2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA092888.1, AB027466.1, BC002707.2 and AA864573.1. On Nov 18, 2010 this sequence version replaced NM_001128325.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 all encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.3242.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAAACC CCAGCCCGGC CGCCGCCCTG GGCAAGGCCC TCTGCGCTCT CCTCCTGGCC 
ACTCTCGGCG CCGCCGGCCA GCCTCTTGGG GGAGAGTCCA TCTGTTCCGC CAGAGCCCCG 
GCCAAATACA GCATCACCTT CACGGGCAAG TGGAGCCAGA CGGCCTTCCC CAAGCAGTAC 
CCCCTGTTCC GCCCCCCTGC GCAGTGGTCT TCGCTGCTGG GGGCCGCGCA TAGCTCCGAC 
TACAGCATGT GGAGGAAGAA CCAGTACGTC AGTAACGGGC TGCGCGACTT TGCGGAGCGC 
GGCGAGGCCT GGGCGCTGAT GAAGGAGATC GAGGCGGCGG GGGAGGCGCT GCAGAGCGTG 
CACGCGGTGT TTTCGGCGCC CGCCGTCCCC AGCGGCACCG GGCAGACGTC GGCGGAGCTG 
GAGGTGCAGC GCAGGCACTC GCTGGTCTCG TTTGTGGTGC GCATCGTGCC CAGCCCCGAC 
TGGTTCGTGG GCGTGGACAG CCTGGACCTG TGCGACGGGG ACCGTTGGCG GGAACAGGCG 
GCGCTGGACC TGTACCCCTA CGACGCCGGG ACGGACAGCG GCTTCACCTT CTCCTCCCCC 
AACTTCGCCA CCATCCCGCA GGACACGGTG ACCGAGATAA CGTCCTCCTC TCCCAGCCAC 
CCGGCCAACT CCTTCTACTA CCCGCGGCTG AAGGCCCTGC CTCCCATCGC CAGGGTGACA 
CTGGTGCGGC TGCGACAGAG CCCCAGGGCC TTCATCCCTC CCGCCCCAGT CCTGCCCAGC 
AGGGACAATG AGATTGTAGA CAGCGCCTCA GTTCCAGAAA CGCCGCTGGA CTGCGAGGTC 
TCCCTGTGGT CGTCCTGGGG ACTGTGCGGA GGCCACTGTG GGAGGCTCGG GACCAAGAGC 
AGGACTCGCT ACGTCCGGGT CCAGCCCGCC AACAACGGGA GCCCCTGCCC CGAGCTCGAA 
GAAGAGGCTG AGTGCGTCCC TGATAACTGC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121797.1
CDS	272..1267
Translation	MENPSPAAALGKALCALLLATLGAAGQPLGGESICSARAPAKYSITFTGKWSQTAFPKQYPLFRPPAQWSSLLGAAHSSDYSMWRKNQYVSNGLRDFAERGEAWALMKEIEAAGEALQSVHAVFSAPAVPSGTGQTSAELEVQRRHSLVSFVVRIVPSPDWFVGVDSLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEITSSSPSHPANSFYYPRLKALPPIARVTLVRLRQSPRAFIPPAPVLPSRDNEIVDSASVPETPLDCEVSLWSSWGLCGGHCGRLGTKSRTRYVRVQPANNGSPCPELEEEAECVPDNCV

Target ORF information:

RefSeq Version	NM_001128325.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spondin 2 (SPON2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128325.2

ORF Insert Sequence:

ATGGAAAACC CCAGCCCGGC CGCCGCCCTG GGCAAGGCCC TCTGCGCTCT CCTCCTGGCC 
ACTCTCGGCG CCGCCGGCCA GCCTCTTGGG GGAGAGTCCA TCTGTTCCGC CAGAGCCCCG 
GCCAAATACA GCATCACCTT CACGGGCAAG TGGAGCCAGA CGGCCTTCCC CAAGCAGTAC 
CCCCTGTTCC GCCCCCCTGC GCAGTGGTCT TCGCTGCTGG GGGCCGCGCA TAGCTCCGAC 
TACAGCATGT GGAGGAAGAA CCAGTACGTC AGTAACGGGC TGCGCGACTT TGCGGAGCGC 
GGCGAGGCCT GGGCGCTGAT GAAGGAGATC GAGGCGGCGG GGGAGGCGCT GCAGAGCGTG 
CACGCGGTGT TTTCGGCGCC CGCCGTCCCC AGCGGCACCG GGCAGACGTC GGCGGAGCTG 
GAGGTGCAGC GCAGGCACTC GCTGGTCTCG TTTGTGGTGC GCATCGTGCC CAGCCCCGAC 
TGGTTCGTGG GCGTGGACAG CCTGGACCTG TGCGACGGGG ACCGTTGGCG GGAACAGGCG 
GCGCTGGACC TGTACCCCTA CGACGCCGGG ACGGACAGCG GCTTCACCTT CTCCTCCCCC 
AACTTCGCCA CCATCCCGCA GGACACGGTG ACCGAGATAA CGTCCTCCTC TCCCAGCCAC 
CCGGCCAACT CCTTCTACTA CCCGCGGCTG AAGGCCCTGC CTCCCATCGC CAGGGTGACA 
CTGGTGCGGC TGCGACAGAG CCCCAGGGCC TTCATCCCTC CCGCCCCAGT CCTGCCCAGC 
AGGGACAATG AGATTGTAGA CAGCGCCTCA GTTCCAGAAA CGCCGCTGGA CTGCGAGGTC 
TCCCTGTGGT CGTCCTGGGG ACTGTGCGGA GGCCACTGTG GGAGGCTCGG GACCAAGAGC 
AGGACTCGCT ACGTCCGGGT CCAGCCCGCC AACAACGGGA GCCCCTGCCC CGAGCTCGAA 
GAAGAGGCTG AGTGCGTCCC TGATAACTGC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPON2 ( NM_012445.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_036577.2 Homo sapiens spondin 2 (SPON2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu107292
Clone ID Related Accession (Same CDS sequence)	NM_012445.4
Accession Version	NM_012445.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-17
Organism	Homo sapiens(human)
Product	spondin-2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092535.4. On Nov 23, 2018 this sequence version replaced NM_012445.3. Transcript Variant: This variant (1) encodes the same protein as variants 2 and 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY358948.1, SRR3476690.188311.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000290902.10/ ENSP00000290902.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAAAACC CCAGCCCGGC CGCCGCCCTG GGCAAGGCCC TCTGCGCTCT CCTCCTGGCC 
ACTCTCGGCG CCGCCGGCCA GCCTCTTGGG GGAGAGTCCA TCTGTTCCGC CAGAGCCCTG 
GCCAAATACA GCATCACCTT CACGGGCAAG TGGAGCCAGA CGGCCTTCCC CAAGCAGTAC 
CCCCTGTTCC GCCCCCCTGC GCAGTGGTCT TCGCTGCTGG GGGCCGCGCA TAGCTCCGAC 
TACAGCATGT GGAGGAAGAA CCAGTACGTC AGTAACGGGC TGCGCGACTT TGCGGAGCGC 
GGCGAGGCCT GGGCGCTGAT GAAGGAGATC GAGGCGGCGG GGGAGGCGCT GCAGAGCGTG 
CACGCGGTGT TTTCGGCGCC CGCCGTCCCC AGCGGCACCG GGCAGACGTC GGCGGAGCTG 
GAGGTGCAGC GCAGGCACTC GCTGGTCTCG TTTGTGGTGC GCATCGTGCC CAGCCCCGAC 
TGGTTCGTGG GCGTGGACAG CCTGGACCTG TGCGACGGGG ACCGTTGGCG GGAACAGGCG 
GCGCTGGACC TGTACCCCTA CGACGCCGGG ACGGACAGCG GCTTCACCTT CTCCTCCCCC 
AACTTCGCCA CCATCCCGCA GGACACGGTG ACCGAGATAA CGTCCTCCTC TCCCAGCCAC 
CCGGCCAACT CCTTCTACTA CCCGCGGCTG AAGGCCCTGC CTCCCATCGC CAGGGTGACA 
CTGGTGCGGC TGCGACAGAG CCCCAGGGCC TTCATCCCTC CCGCCCCAGT CCTGCCCAGC 
AGGGACAATG AGATTGTAGA CAGCGCCTCA GTTCCAGAAA CGCCGCTGGA CTGCGAGGTC 
TCCCTGTGGT CGTCCTGGGG ACTGTGCGGA GGCCACTGTG GGAGGCTCGG GACCAAGAGC 
AGGACTCGCT ACGTCCGGGT CCAGCCCGCC AACAACGGGA GCCCCTGCCC CGAGCTCGAA 
GAAGAGGCTG AGTGCGTCCC TGATAACTGC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036577.2
CDS	44..1039
Translation	MENPSPAAALGKALCALLLATLGAAGQPLGGESICSARALAKYSITFTGKWSQTAFPKQYPLFRPPAQWSSLLGAAHSSDYSMWRKNQYVSNGLRDFAERGEAWALMKEIEAAGEALQSVHAVFSAPAVPSGTGQTSAELEVQRRHSLVSFVVRIVPSPDWFVGVDSLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEITSSSPSHPANSFYYPRLKALPPIARVTLVRLRQSPRAFIPPAPVLPSRDNEIVDSASVPETPLDCEVSLWSSWGLCGGHCGRLGTKSRTRYVRVQPANNGSPCPELEEEAECVPDNCV

Target ORF information:

RefSeq Version	NM_012445.4
Organism	Homo sapiens(human)
Definition	Homo sapiens spondin 2 (SPON2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012445.4

ORF Insert Sequence:

ATGGAAAACC CCAGCCCGGC CGCCGCCCTG GGCAAGGCCC TCTGCGCTCT CCTCCTGGCC 
ACTCTCGGCG CCGCCGGCCA GCCTCTTGGG GGAGAGTCCA TCTGTTCCGC CAGAGCCCTG 
GCCAAATACA GCATCACCTT CACGGGCAAG TGGAGCCAGA CGGCCTTCCC CAAGCAGTAC 
CCCCTGTTCC GCCCCCCTGC GCAGTGGTCT TCGCTGCTGG GGGCCGCGCA TAGCTCCGAC 
TACAGCATGT GGAGGAAGAA CCAGTACGTC AGTAACGGGC TGCGCGACTT TGCGGAGCGC 
GGCGAGGCCT GGGCGCTGAT GAAGGAGATC GAGGCGGCGG GGGAGGCGCT GCAGAGCGTG 
CACGCGGTGT TTTCGGCGCC CGCCGTCCCC AGCGGCACCG GGCAGACGTC GGCGGAGCTG 
GAGGTGCAGC GCAGGCACTC GCTGGTCTCG TTTGTGGTGC GCATCGTGCC CAGCCCCGAC 
TGGTTCGTGG GCGTGGACAG CCTGGACCTG TGCGACGGGG ACCGTTGGCG GGAACAGGCG 
GCGCTGGACC TGTACCCCTA CGACGCCGGG ACGGACAGCG GCTTCACCTT CTCCTCCCCC 
AACTTCGCCA CCATCCCGCA GGACACGGTG ACCGAGATAA CGTCCTCCTC TCCCAGCCAC 
CCGGCCAACT CCTTCTACTA CCCGCGGCTG AAGGCCCTGC CTCCCATCGC CAGGGTGACA 
CTGGTGCGGC TGCGACAGAG CCCCAGGGCC TTCATCCCTC CCGCCCCAGT CCTGCCCAGC 
AGGGACAATG AGATTGTAGA CAGCGCCTCA GTTCCAGAAA CGCCGCTGGA CTGCGAGGTC 
TCCCTGTGGT CGTCCTGGGG ACTGTGCGGA GGCCACTGTG GGAGGCTCGG GACCAAGAGC 
AGGACTCGCT ACGTCCGGGT CCAGCCCGCC AACAACGGGA GCCCCTGCCC CGAGCTCGAA 
GAAGAGGCTG AGTGCGTCCC TGATAACTGC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The extracellular matrix protein mindin attenuates colon cancer progression by blocking angiogenesis via Egr-1-mediated regulation.
Oncogene(2017 Oct)
Wang LF,Liu YS,Yang B,Li P,Cheng XS,Xiao CX,Liu JJ,Li S,Ren JL,Guleng B

Elevated spondin-2 expression correlates with progression and prognosis in gastric cancer.
Oncotarget8(6)10416-10424(2017 Feb)
Jin C,Lin JR,Ma L,Song Y,Shi YX,Jiang P,Dong Y,Li XS

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

SPON2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報