SELENOF cDNA ORF clone, Macaca mulatta(Rhesus monkey)

Gene
Clones
gRNAs

Summary

Gene Symbol	SELENOF
Entrez Gene ID	712469
Full Name	selenoprotein F
Synonyms	SEP15
Gene Type	protein-coding
Organism	Macaca mulatta(Rhesus monkey)
Summary	The protein encoded by this gene belongs to the SEP15/selenoprotein M family. The exact function of this protein is not known; however, it has been found to associate with UDP-glucose:glycoprotein glucosyltransferase (UGTR), an endoplasmic reticulum(ER)-resident protein, which is involved in the quality control of protein folding. The association with UGTR retains this protein in the ER, where it may play a role in protein folding. It has also been suggested to have a role in cancer etiology. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001346968.1	NP_001333897.1	selenoprotein F isoform 2 precursor
NM_001159416.2	NP_001152888.1	selenoprotein F isoform 1 precursor

Pathways from BioSystems

Homologies

	SEP15	NP_001152888.1
Bos taurus (cattle)	SEP15	NP_001029931.2
Gallus gallus (chicken)	SEP15	NP_001012944.2
Arabidopsis thaliana (thale cress)	AT1G05720	NP_563747.1
Xenopus tropicalis (tropical clawed frog)	sep15	NP_001165182.1
Homo sapiens (human)	SEP15	NP_004252.2
Pan troglodytes (chimpanzee)	SEP15	NP_001139315.1
Rattus norvegicus (Norway rat)	Sep15	NP_579831.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004986	XP_315090.2
Oryza sativa (rice)	Os01g0894500	NP_001045069.1
Canis lupus familiaris (dog)	SEP15	NP_001108232.1
Mus musculus (house mouse)	Sep15	NP_444332.1
Danio rerio (zebrafish)	sep15	NP_840079.1
Drosophila melanogaster (fruit fly)	CG7484	NP_649000.1
Caenorhabditis elegans (roundworm)	Y76B12C.3	NP_500152.1

Gene Ontology
Bibliography

Related articles in PubMed

A new rhesus macaque assembly and annotation for next-generation sequencing analyses.
Zimin AV, Cornish AS, Maudhoo MD, Gibbs RM, Zhang X, Pandey S, Meehan DT, Wipfler K, Bosinger SE, Johnson ZP, Tharp GK, Mar?ais G, Roberts M, Ferguson B, Fox HS, Treangen T, Salzberg SL, Yorke JA, Norgren RB Jr
Biology direct9(1)20(2014 Oct)

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SELENOF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SELENOF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMb03104	NM_001159416.2 Latest version!	Macaca mulatta selenoprotein F (SELENOF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OMb57585	NM_001346968.1 Latest version!	Macaca mulatta selenoprotein F (SELENOF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SELENOF ( NM_001159416.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> NP_001152888.1 Macaca mulatta selenoprotein F (SELENOF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMb03104
Clone ID Related Accession (Same CDS sequence)	NM_001159416.2
Accession Version	NM_001159416.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-10
Organism	Macaca mulatta(Rhesus monkey)
Product	selenoprotein F isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from QNVO02000012.1, JV528708.1 and JV704574.1. On Nov 9, 2016 this sequence version replaced NM_001159416.1. Transcript Variant: This variant (1) represents the predominant transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: JV704574.1, JU332145.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4688315, SAMEA4688316 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTAGCGA TGGCGGCTGG GCTGAGTGGG TGGCTGGTGC CGACGTTTGG GCTACGGTTG 
TTGTTGGCGA CTGTGCTTCA AGCGGTTTCT GCTTTTGGGG CAGAGTTTTC ATCAGAGGCA 
TGCAGAGAGT TAGGCTTTTC TAGCAACTTG CTCTGCAGCT CTTGTGATCT TCTCGGACAG 
TTCAACCTGC TTCAGCTGGA TCCTGATTGC AGAGGATGCT GTCAGGAGGA AGCACAATTT 
GAAACCAAAA AGCTGTATGC AGGAGCTATT CTTGAAGTTT GTGGATGAAA ATTGGGAAGG 
TTCCCTCAAG TCCAAGCTTT CGTTAGGAGT GATAAACCCA AATTGTTCAG AGGACTACAA 
ATCAAGTATG TCCGTGGTTC AGACCCTATA TTAAAGCTTT TGGATGACAA TGGGAACATT 
GCTGAAGAAC TAAGCATTCT CAAATGGAAC ACAGACAGTG TAGAAGAATT CCTGAGTGAA 
AAGTTGGAAC GCATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001152888.1
CDS	261..758
Translation	MVAMAAGLSGWLVPTFGLRLLLATVLQAVSAFGAEFSSEACRELGFSSNLLCSSCDLLGQFNLLQLDPDCRGCCQEEAQFETKKLYAGAILEVCG*KLGRFPQVQAFVRSDKPKLFRGLQIKYVRGSDPILKLLDDNGNIAEELSILKWNTDSVEEFLSEKLERI

Target ORF information:

RefSeq Version	NM_001159416.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta selenoprotein F (SELENOF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159416.2

ORF Insert Sequence:

ATGGTAGCGA TGGCGGCTGG GCTGAGTGGG TGGCTGGTGC CGACGTTTGG GCTACGGTTG 
TTGTTGGCGA CTGTGCTTCA AGCGGTTTCT GCTTTTGGGG CAGAGTTTTC ATCAGAGGCA 
TGCAGAGAGT TAGGCTTTTC TAGCAACTTG CTCTGCAGCT CTTGTGATCT TCTCGGACAG 
TTCAACCTGC TTCAGCTGGA TCCTGATTGC AGAGGATGCT GTCAGGAGGA AGCACAATTT 
GAAACCAAAA AGCTGTATGC AGGAGCTATT CTTGAAGTTT GTGGATGAAA ATTGGGAAGG 
TTCCCTCAAG TCCAAGCTTT CGTTAGGAGT GATAAACCCA AATTGTTCAG AGGACTACAA 
ATCAAGTATG TCCGTGGTTC AGACCCTATA TTAAAGCTTT TGGATGACAA TGGGAACATT 
GCTGAAGAAC TAAGCATTCT CAAATGGAAC ACAGACAGTG TAGAAGAATT CCTGAGTGAA 
AAGTTGGAAC GCATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SELENOF ( NM_001346968.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> NP_001333897.1 Macaca mulatta selenoprotein F (SELENOF), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMb57585
Clone ID Related Accession (Same CDS sequence)	NM_001346968.1
Accession Version	NM_001346968.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-10
Organism	Macaca mulatta(Rhesus monkey)
Product	selenoprotein F isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from QNVO02000012.1, JU337415.1 and JV704574.1. Transcript Variant: This variant (2) lacks an exon in the 3' coding region, which results in a frameshift compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: JU337415.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4688315, SAMEA4688317 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTAGCGA TGGCGGCTGG GCTGAGTGGG TGGCTGGTGC CGACGTTTGG GCTACGGTTG 
TTGTTGGCGA CTGTGCTTCA AGCGGTTTCT GCTTTTGGGG CAGAGTTTTC ATCAGAGGCA 
TGCAGAGAGT TAGGCTTTTC TAGCAACTTG CTCTGCAGCT CTTGTGATCT TCTCGGACAG 
TTCAACCTGC TTCAGCTGGA TCCTGATTGC AGAGGATGCT GTCAGGAGGA AGCACAATTT 
GAAACCAAAA AGCTGTATGC AGGAGCTATT CTTGAAGTTT GTGGATGAAA ATTGGGAAGG 
TTCCCTCAAG TCCAAGTATG TCCGTGGTTC AGACCCTATA TTAAAGCTTT TGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333897.1
CDS	261..617
Translation	MVAMAAGLSGWLVPTFGLRLLLATVLQAVSAFGAEFSSEACRELGFSSNLLCSSCDLLGQFNLLQLDPDCRGCCQEEAQFETKKLYAGAILEVCG*KLGRFPQVQVCPWFRPYIKAFG

Target ORF information:

RefSeq Version	NM_001346968.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta selenoprotein F (SELENOF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346968.1

ORF Insert Sequence:

ATGGTAGCGA TGGCGGCTGG GCTGAGTGGG TGGCTGGTGC CGACGTTTGG GCTACGGTTG 
TTGTTGGCGA CTGTGCTTCA AGCGGTTTCT GCTTTTGGGG CAGAGTTTTC ATCAGAGGCA 
TGCAGAGAGT TAGGCTTTTC TAGCAACTTG CTCTGCAGCT CTTGTGATCT TCTCGGACAG 
TTCAACCTGC TTCAGCTGGA TCCTGATTGC AGAGGATGCT GTCAGGAGGA AGCACAATTT 
GAAACCAAAA AGCTGTATGC AGGAGCTATT CTTGAAGTTT GTGGATGAAA ATTGGGAAGG 
TTCCCTCAAG TCCAAGTATG TCCGTGGTTC AGACCCTATA TTAAAGCTTT TGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A new rhesus macaque assembly and annotation for next-generation sequencing analyses.
Biology direct9(1)20(2014 Oct)
Zimin AV,Cornish AS,Maudhoo MD,Gibbs RM,Zhang X,Pandey S,Meehan DT,Wipfler K,Bosinger SE,Johnson ZP,Tharp GK,Mar?ais G,Roberts M,Ferguson B,Fox HS,Treangen T,Salzberg SL,Yorke JA,Norgren RB Jr

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

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SELENOF cDNA ORF clone, Macaca mulatta(Rhesus monkey)

Gene

Clones

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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