SELENOF cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	SELENOF
Entrez Gene ID	424521
Full Name	selenoprotein F
Synonyms	SEP15
General protein information	Preferred Names selenoprotein F Names selenoprotein F 15 kDa selenoprotein
Gene Type	protein-coding
Organism	Gallus gallus(chicken)
Summary	The protein encoded by this gene belongs to the SEP15/selenoprotein M family. The exact function of this protein is not known; however, it has been found to associate with UDP-glucose:glycoprotein glucosyltransferase (UGTR), an endoplasmic reticulum(ER)-resident protein, which is involved in the quality control of protein folding. The association with UGTR retains this protein in the ER, where it may play a role in protein folding. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. [provided by RefSeq, Nov 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001012926.3	NP_001012944.2	selenoprotein F precursor

Pathways from BioSystems

Homologies

	SEP15	NP_001152888.1
Bos taurus (cattle)	SEP15	NP_001029931.2
Gallus gallus (chicken)	SEP15	NP_001012944.2
Arabidopsis thaliana (thale cress)	AT1G05720	NP_563747.1
Xenopus tropicalis (tropical clawed frog)	sep15	NP_001165182.1
Homo sapiens (human)	SEP15	NP_004252.2
Pan troglodytes (chimpanzee)	SEP15	NP_001139315.1
Rattus norvegicus (Norway rat)	Sep15	NP_579831.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004986	XP_315090.2
Oryza sativa (rice)	Os01g0894500	NP_001045069.1
Canis lupus familiaris (dog)	SEP15	NP_001108232.1
Mus musculus (house mouse)	Sep15	NP_444332.1
Danio rerio (zebrafish)	sep15	NP_840079.1
Drosophila melanogaster (fruit fly)	CG7484	NP_649000.1
Caenorhabditis elegans (roundworm)	Y76B12C.3	NP_500152.1

Gene Ontology
Bibliography

Related articles in PubMed

Dietary Selenium Status Regulates the Transcriptions of Selenoproteome and Activities of Selenoenzymes in Chicken Kidney at Low or Super-nutritional Levels.
Xu JX, Zhang C, Cao CY, Zhu SY, Li H, Sun YC, Li JL
Biological trace element research170(2)438-48(2016 Apr)

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

Non-coding RNAs revealed during identification of genes involved in chicken immune responses.
Ahanda ML, Ruby T, Wittzell H, Bed'Hom B, Chauss? AM, Morin V, Oudin A, Chevalier C, Young JR, Zoorob R
Immunogenetics61(1)55-70(2009 Jan)

Analysis of chicken embryonic gonad expressed sequenced tags.
Shin JH, Kim H, Lim D, Jeon M, Han BK, Park TS, Kim JK, Lillehoj HS, Cho BW, Han JY
Animal genetics37(1)85-6(2006 Feb)

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Caldwell RB, Kierzek AM, Arakawa H, Bezzubov Y, Zaim J, Fiedler P, Kutter S, Blagodatski A, Kostovska D, Koter M, Plachy J, Carninci P, Hayashizaki Y, Buerstedde JM
Genome biology6(1)R6(2005)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

At super-nutritional Se, most selenoproteins were upregulated in chicken kidney, but Sepp2 and Sep15 was only upregulated in Se excess (5.0 mg/kg) bird.
Title: Dietary Selenium Status Regulates the Transcriptions of Selenoproteome and Activities of Selenoenzymes in Chicken Kidney at Low or Super-nutritional Levels.

The following SELENOF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SELENOF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa26438	NM_001012926.3 Latest version!	Gallus gallus selenoprotein F (SELENOF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SELENOF ( NM_001012926.3 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001012944.2 Gallus gallus selenoprotein F (SELENOF), mRNA.

Price & Availability↑

CloneID	OGa26438
Clone ID Related Accession (Same CDS sequence)	NM_001012926.3
Accession Version	NM_001012926.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-20
Organism	Gallus gallus(chicken)
Product	selenoprotein F precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX935466.1, BU113839.1, CK608144.1, CF250911.1, DR430574.1 and AJ726721.1. On Nov 13, 2016 this sequence version replaced NM_001012926.2. ##Evidence-Data-START## Transcript exon combination :: AJ719491.1, HAEK01012774.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CCGCCGAGTT GGCCGCCCTG GTGCGCTGCT GGCTCTGCCT GCTGCTGGGG 
CTGCCCGCGA TAAATGTATA TGGAGCACAG CTGTCATCAG AAGCTTGCAG GGAACTTGGC 
TTCTCCAGTA ACTTGCTCTG CAGCTCCTGC AACCTTCTTG GACAGTTCAG TCTGAATCAG 
TTGGATCCAT TCTGTAGGCA GTGCTGCCAA GAAGAAGCTC AGCTGGAAAC CAGAAAGCTT 
TATGCAGGAG CTGTCCTAGA AGTATGTGGA TGAAAATTGG GAAGGTTCCC TCAAGTCCAG 
GCTTTTGTCA GGAGTGACAA GCCTAAGCTC TTCAGGGGAC TGCAAATCAA GTACGTGCGT 
GGTTCTGACC CTGTACTGAA GCTGCTGGAC GACAGTGGGA ACATTGCAGA AGAACTGAGC 
ATCCTCAAGT GGAATACAGA CAGCGTGGAA GAGTTTCTAA GTGAAAAATT AGAGCGTCTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012944.2
CDS	11..493
Translation	MAAAAELAALVRCWLCLLLGLPAINVYGAQLSSEACRELGFSSNLLCSSCNLLGQFSLNQLDPFCRQCCQEEAQLETRKLYAGAVLEVCG*KLGRFPQVQAFVRSDKPKLFRGLQIKYVRGSDPVLKLLDDSGNIAEELSILKWNTDSVEEFLSEKLERL

Target ORF information:

RefSeq Version	NM_001012926.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus selenoprotein F (SELENOF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012926.3

ORF Insert Sequence:

ATGGCGGCGG CCGCCGAGTT GGCCGCCCTG GTGCGCTGCT GGCTCTGCCT GCTGCTGGGG 
CTGCCCGCGA TAAATGTATA TGGAGCACAG CTGTCATCAG AAGCTTGCAG GGAACTTGGC 
TTCTCCAGTA ACTTGCTCTG CAGCTCCTGC AACCTTCTTG GACAGTTCAG TCTGAATCAG 
TTGGATCCAT TCTGTAGGCA GTGCTGCCAA GAAGAAGCTC AGCTGGAAAC CAGAAAGCTT 
TATGCAGGAG CTGTCCTAGA AGTATGTGGA TGAAAATTGG GAAGGTTCCC TCAAGTCCAG 
GCTTTTGTCA GGAGTGACAA GCCTAAGCTC TTCAGGGGAC TGCAAATCAA GTACGTGCGT 
GGTTCTGACC CTGTACTGAA GCTGCTGGAC GACAGTGGGA ACATTGCAGA AGAACTGAGC 
ATCCTCAAGT GGAATACAGA CAGCGTGGAA GAGTTTCTAA GTGAAAAATT AGAGCGTCTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dietary Selenium Status Regulates the Transcriptions of Selenoproteome and Activities of Selenoenzymes in Chicken Kidney at Low or Super-nutritional Levels.
Biological trace element research170(2)438-48(2016 Apr)
Xu JX,Zhang C,Cao CY,Zhu SY,Li H,Sun YC,Li JL

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

Non-coding RNAs revealed during identification of genes involved in chicken immune responses.
Immunogenetics61(1)55-70(2009 Jan)
Ahanda ML,Ruby T,Wittzell H,Bed'Hom B,Chauss? AM,Morin V,Oudin A,Chevalier C,Young JR,Zoorob R

Analysis of chicken embryonic gonad expressed sequenced tags.
Animal genetics37(1)85-6(2006 Feb)
Shin JH,Kim H,Lim D,Jeon M,Han BK,Park TS,Kim JK,Lillehoj HS,Cho BW,Han JY

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Genome biology6(1)R6(2005)
Caldwell RB,Kierzek AM,Arakawa H,Bezzubov Y,Zaim J,Fiedler P,Kutter S,Blagodatski A,Kostovska D,Koter M,Plachy J,Carninci P,Hayashizaki Y,Buerstedde JM

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SELENOF cDNA ORF clone, Gallus gallus(chicken)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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