TSC2 gene cDNA ORF clone, Macaca mulatta(Rhesus monkey)

mRNA	Protein	Name
XM_015125349.1	XP_014980835.1	tuberin isoform X1
XM_015125358.1	XP_014980844.1	tuberin isoform X12
XM_015125359.1	XP_014980845.1	tuberin isoform X13
XM_028841486.1	XP_028697319.1	tuberin isoform X3
XM_028841492.1	XP_028697325.1	tuberin isoform X12
XM_015125350.1	XP_014980836.1	tuberin isoform X2
XM_015125351.1	XP_014980837.1	tuberin isoform X4
XM_015125353.1	XP_014980839.1	tuberin isoform X7
XM_015125354.1	XP_014980840.1	tuberin isoform X8
XM_015125357.1	XP_014980843.1	tuberin isoform X11
XM_028841489.1	XP_028697322.1	tuberin isoform X5
XM_001083256.4	XP_001083256.3	tuberin isoform X7
XM_001083508.4	XP_001083508.3	tuberin isoform X9
XM_015125355.1	XP_014980841.1	tuberin isoform X9
XM_028841490.1	XP_028697323.1	tuberin isoform X10
XM_028841491.1	XP_028697324.1	tuberin isoform X11
XM_015125356.2	XP_014980842.2	tuberin isoform X13
XM_015125357.2	XP_014980843.2	tuberin isoform X14
XM_001083256.3	XP_001083256.2	tuberin isoform X3
XM_015125352.1	XP_014980838.1	tuberin isoform X5
XM_015125356.1	XP_014980842.1	tuberin isoform X10
XM_028841485.1	XP_028697318.1	tuberin isoform X2
XM_028841487.1	XP_028697320.1	tuberin isoform X4
XM_015125349.2	XP_014980835.2	tuberin isoform X6
XM_015125352.2	XP_014980838.2	tuberin isoform X8
XM_001083508.3	XP_001083508.2	tuberin isoform X6
XM_028841484.1	XP_028697317.1	tuberin isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMb81166	XM_028841486.1 Latest version!	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMb81167	XM_028841487.1 Latest version!	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMb81168	XM_028841489.1 Latest version!	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMb81169	XM_015125349.2 Latest version!	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMb81170	XM_001083256.4 Latest version!	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMb81171	XM_015125352.2 Latest version!	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMb81172	XM_001083508.4 Latest version!	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMb81173	XM_028841490.1 Latest version!	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMb81174	XM_028841491.1 Latest version!	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMb81175	XM_028841492.1 Latest version!	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMb81176	XM_015125356.2 Latest version!	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMb81177	XM_015125357.2 Latest version!	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMb81164	XM_028841484.1 Latest version!	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMb81165	XM_028841485.1 Latest version!	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMb54728	XM_015125349.1 Latest version!	Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OMb54729	XM_015125350.1 Latest version!	Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OMb54730	XM_001083256.3 Latest version!	Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OMb54731	XM_015125351.1 Latest version!	Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OMb54732	XM_015125352.1 Latest version!	Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OMb54733	XM_001083508.3 Latest version!	Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OMb54734	XM_015125353.1 Latest version!	Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OMb54735	XM_015125354.1 Latest version!	Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OMb54736	XM_015125355.1 Latest version!	Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OMb54737	XM_015125356.1 Latest version!	Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OMb54738	XM_015125357.1 Latest version!	Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OMb54739	XM_015125358.1 Latest version!	Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OMb54740	XM_015125359.1 Latest version!	Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

TSC2 ( XM_028841486.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028697319.1 Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMb81166
Clone ID Related Accession (Same CDS sequence)	XM_028841486.1
Accession Version	XM_028841486.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAGGCTCCGT GGCGGCCCTC CGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
CCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAGCAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACCGCCGAA 
ATCCTGAGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTATATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCGTCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCGGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGTC TGAGGATAGC CAGACCCCCC AAACAAGGCT TGAATAACTC TCCACCCGTG 
AAAGAATTCA AGGAGAGCTC TGCAGCCGAG GCCTTCCGGT GCCGCAGCAT CAGTGTGTCT 
GAACATGTGG TCCGCAGCAG GATACAGACG TCCCTCACCA GTGCCAGCCT GGGGTCTGCA 
GACGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA GCCTCCATCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCCCGATAC GTCTTCTCCA ACTTCACAGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTGGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGTGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGCAT GAATGTGAGA 
CAGACGAAGG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGTTC TATGTCAGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAT 
GTGCCGGCCT CCCAGTTCCC GGGCAGTGCC GCTTCTCCAG GACCACAGAC TGCACCAGCC 
GCGAAGCCTG AGAAGGCCTC GGCCGGCGCC CGGCTTCCTG TGCAGGAGAA GACGAACCTG 
GCCGCCTACG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CAGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCGCTG TCGGTGCCAG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGACTCC GCCGTGGTCA TGGAGGAGGG AAGTCCTGGC 
GAGGTTCCTG TGCCAGTGGA GCCCCCAGGA TTGGAGGACT TTGAGGCGGC GCTAGGCGTG 
GACAGGCGCA CAGACGCCTA CAGCAGGTCG TCCTCAACCT CCAGCCAGGA GGAGAAGTCA 
TTCCACGCAG AGGAGCTGGT TGCCGGGGGA ATCCCCATTG AACGAGCCGT CTCCTCGGAG 
GGTGGCCGGC CCTCTGTGGA CCTCTCCTTC CAGCCCTCAC AGCCCCTGAG CAAGTCTAGC 
TCCTCTCCTG AGCTGCAGAC CCTGCAGGAC ATCCTCGGGG ACCCTGGGGA CAAGGCTGAC 
GTGGGCCGGC TGAGCCCTGA GGTTAAGGCC CGTTCACAGT CAGGGATCCT GGACGGGGAA 
AGTGCTGCCT GGCCAGCCTC GGGCGAAGAC AGTCGGGGCC AGCCCGAGGG TCCCTTGCCT 
TCCAGCTCCC CCCGCTCACC CAGTGGCCTC CGGCCCCGCG GTTACACCAT CTCCGACTCG 
GCCCCATCAC GCAGGGGCAG GAGGGTAGAA AGGGATGCCT TTAAGAGCAG AGCCACAGCT 
TCCAATGCAG AGAAAGTGCC AGGCATCAAC CCCAGGTGGG CCTCTTGCTT CCGGGCGGGG 
CTCCTGATAC CATCCTGCAG GAACCTGGTG CCTCAGTTGC CCCAGGCCGA GCAGGCTGGG 
GAGGGTCCTC GCCTGTGCCC TAGGGCTGGC TGGAGCCCCT GGCAAGGCGG TGGAGTGGGA 
GATGGCCAGG TTCTGTGTTC CTCCCTGGGC TGTGGCTGCC CTGGCCAGGC CTTCACCTGG 
GTGCCCACTG TCTCCTCCCT GTGCAGTTTC GTGTTCCTGC AGCTCTACCA TTCGCCTTTC 
TTTGGCGACG AGTCAAACAA GCCAATCCTG CTGCCCAATG AGTCACAGTC CTTTGAGCGG 
TCGGTGCAGC TCCTCGACCA GATCCCATCA TACGACACCC ACAAGATCGC CGTCCTGTAT 
GTGGGAGAAG GCCAGAGCAA CAGTGAGCTC GCCATCCTGT CCAATGAGCA TGGCTCCTAC 
AGGTACACGG AGTTCCTGAC GGGCCTGGGC CGGCTCATCG AGCTCAAGGA CTGCCAGCCA 
GACAAGGTGT ACCTGGGCGG CCTGGACGTG TGTGGTGAGG ACGGCCAGTT CACCTACTGC 
TGGCACGACG ACATCATGCA AGCCGTCTTC CACATCGCCA CCCTGATGCC CACCAAGGAC 
GTGGACAAGC ATCGTTGCGA CAAGAAGCGC CACCTGGGCA ACGATTTCGT GTCCATTGTC 
TACAATGACT CTGGCGAGGA CTTCAAGCTC GGCACCATCA AGGGCCAGTT CAACTTTGTC 
CACGTGATCA TCACCCCGCT GGATTATGAG TGCAACCTGG TGTCCCTGCA GTGCAGGAAA 
GACATGGAGG GCCTTGTGGA CACCAGCGTG GCCAAGATCG TGTCTGACCG CAACCTGCCT 
TTTGTGGCCC GTCAGATGGC CCTGCATGCA AACATGGCCT CACAGGTGCA CCACAGCCGC 
TCCAACCCCA CCGACATCTA CCCCTCCAAG TGGATTGCCC GGCTCCGCCA CATCAAGAGA 
CTCCGCCAGC GGATCTGCGA GGAAGCCGCC TACTCCAACC CCAGCCTGCC TCTGGTGCAC 
CCCCCATCCC ATACCAAAGG GCCTGCACAG ACTCCAGCCG AGCCCACGCC TGGCTACGAG 
ATGGGCCAGC GGAAGCGCCT CATCTCCTCT GTGGAGGACT TCACCGAGTT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028697319.1
CDS	204..6200
Translation	MLRSGRGATCCSFSPPGRRGLLPTPPRSSPGSQAPVPRQHPGAQGVHAGLLRTTHPGGPRGPRHVTQGSPAFPLEGCLELQLPAGSVAALRAAPLPEVRVPLPAASGARGVRLSPRRGGPARGGAAREGCSGASWPTMAKPASKDSGLKEKFRILFGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLSNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIIQGQGERLGVLRALFFKVIKDYPSNEDLHERLDVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTDSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASNAEKVPGINPRWASCFRAGLLIPSCRNLVPQLPQAEQAGEGPRLCPRAGWSPWQGGGVGDGQVLCSSLGCGCPGQAFTWVPTVSSLCSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKGPAQTPAEPTPGYEMGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_028841486.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028841486.1

ORF Insert Sequence:

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAGGCTCCGT GGCGGCCCTC CGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
CCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAGCAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACCGCCGAA 
ATCCTGAGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTATATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCGTCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCGGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGTC TGAGGATAGC CAGACCCCCC AAACAAGGCT TGAATAACTC TCCACCCGTG 
AAAGAATTCA AGGAGAGCTC TGCAGCCGAG GCCTTCCGGT GCCGCAGCAT CAGTGTGTCT 
GAACATGTGG TCCGCAGCAG GATACAGACG TCCCTCACCA GTGCCAGCCT GGGGTCTGCA 
GACGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA GCCTCCATCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCCCGATAC GTCTTCTCCA ACTTCACAGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTGGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGTGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGCAT GAATGTGAGA 
CAGACGAAGG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGTTC TATGTCAGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAT 
GTGCCGGCCT CCCAGTTCCC GGGCAGTGCC GCTTCTCCAG GACCACAGAC TGCACCAGCC 
GCGAAGCCTG AGAAGGCCTC GGCCGGCGCC CGGCTTCCTG TGCAGGAGAA GACGAACCTG 
GCCGCCTACG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CAGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCGCTG TCGGTGCCAG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGACTCC GCCGTGGTCA TGGAGGAGGG AAGTCCTGGC 
GAGGTTCCTG TGCCAGTGGA GCCCCCAGGA TTGGAGGACT TTGAGGCGGC GCTAGGCGTG 
GACAGGCGCA CAGACGCCTA CAGCAGGTCG TCCTCAACCT CCAGCCAGGA GGAGAAGTCA 
TTCCACGCAG AGGAGCTGGT TGCCGGGGGA ATCCCCATTG AACGAGCCGT CTCCTCGGAG 
GGTGGCCGGC CCTCTGTGGA CCTCTCCTTC CAGCCCTCAC AGCCCCTGAG CAAGTCTAGC 
TCCTCTCCTG AGCTGCAGAC CCTGCAGGAC ATCCTCGGGG ACCCTGGGGA CAAGGCTGAC 
GTGGGCCGGC TGAGCCCTGA GGTTAAGGCC CGTTCACAGT CAGGGATCCT GGACGGGGAA 
AGTGCTGCCT GGCCAGCCTC GGGCGAAGAC AGTCGGGGCC AGCCCGAGGG TCCCTTGCCT 
TCCAGCTCCC CCCGCTCACC CAGTGGCCTC CGGCCCCGCG GTTACACCAT CTCCGACTCG 
GCCCCATCAC GCAGGGGCAG GAGGGTAGAA AGGGATGCCT TTAAGAGCAG AGCCACAGCT 
TCCAATGCAG AGAAAGTGCC AGGCATCAAC CCCAGGTGGG CCTCTTGCTT CCGGGCGGGG 
CTCCTGATAC CATCCTGCAG GAACCTGGTG CCTCAGTTGC CCCAGGCCGA GCAGGCTGGG 
GAGGGTCCTC GCCTGTGCCC TAGGGCTGGC TGGAGCCCCT GGCAAGGCGG TGGAGTGGGA 
GATGGCCAGG TTCTGTGTTC CTCCCTGGGC TGTGGCTGCC CTGGCCAGGC CTTCACCTGG 
GTGCCCACTG TCTCCTCCCT GTGCAGTTTC GTGTTCCTGC AGCTCTACCA TTCGCCTTTC 
TTTGGCGACG AGTCAAACAA GCCAATCCTG CTGCCCAATG AGTCACAGTC CTTTGAGCGG 
TCGGTGCAGC TCCTCGACCA GATCCCATCA TACGACACCC ACAAGATCGC CGTCCTGTAT 
GTGGGAGAAG GCCAGAGCAA CAGTGAGCTC GCCATCCTGT CCAATGAGCA TGGCTCCTAC 
AGGTACACGG AGTTCCTGAC GGGCCTGGGC CGGCTCATCG AGCTCAAGGA CTGCCAGCCA 
GACAAGGTGT ACCTGGGCGG CCTGGACGTG TGTGGTGAGG ACGGCCAGTT CACCTACTGC 
TGGCACGACG ACATCATGCA AGCCGTCTTC CACATCGCCA CCCTGATGCC CACCAAGGAC 
GTGGACAAGC ATCGTTGCGA CAAGAAGCGC CACCTGGGCA ACGATTTCGT GTCCATTGTC 
TACAATGACT CTGGCGAGGA CTTCAAGCTC GGCACCATCA AGGGCCAGTT CAACTTTGTC 
CACGTGATCA TCACCCCGCT GGATTATGAG TGCAACCTGG TGTCCCTGCA GTGCAGGAAA 
GACATGGAGG GCCTTGTGGA CACCAGCGTG GCCAAGATCG TGTCTGACCG CAACCTGCCT 
TTTGTGGCCC GTCAGATGGC CCTGCATGCA AACATGGCCT CACAGGTGCA CCACAGCCGC 
TCCAACCCCA CCGACATCTA CCCCTCCAAG TGGATTGCCC GGCTCCGCCA CATCAAGAGA 
CTCCGCCAGC GGATCTGCGA GGAAGCCGCC TACTCCAACC CCAGCCTGCC TCTGGTGCAC 
CCCCCATCCC ATACCAAAGG GCCTGCACAG ACTCCAGCCG AGCCCACGCC TGGCTACGAG 
ATGGGCCAGC GGAAGCGCCT CATCTCCTCT GTGGAGGACT TCACCGAGTT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_028841487.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028697320.1 Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X5, mRNA.

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CloneID	OMb81167
Clone ID Related Accession (Same CDS sequence)	XM_028841487.1
Accession Version	XM_028841487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAGGCTCCGT GGCGGCCCTC CGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
CCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAGCAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACCGCCGAA 
ATCCTGAGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTATATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCGTCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCGGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGCA GGATACAGAC GTCCCTCACC AGTGCCAGCC TGGGGTCTGC AGACGAGAAC 
TCCGTGGCCC AGGCTGACGA TAGCCTGAAA AGCCTCCATC TGGAGCTCAC GGAAACCTGT 
CTGGACATGA TGGCCCGATA CGTCTTCTCC AACTTCACAG CTGTCCCGAA GAGGTCTCCT 
GTGGGCGAGT TCCTCCTGGC GGGTGGCAGG ACCAAAACCT GGCTGGTTGG GAACAAGCTT 
GTCACTGTGA CGACGAGTGT GGGAACCGGG ACCCGGTCGT TACTAGGCCT GGACTCGGGG 
GAGCTGCAGT CCGGCCCGGA GTCGAGCTCC AGCCCCGGCA TGAATGTGAG ACAGACGAAG 
GAGGCGCCAG CCAAGCTGGA GTCCCAGGCT GGGCAGCAGG TGTCCCGTGG GGCCCGGGAC 
CGGGTCCGTT CTATGTCAGG GGGCCATGGT CTTCGAGTTG GCGCCCTGGA TGTGCCGGCC 
TCCCAGTTCC CGGGCAGTGC CGCTTCTCCA GGACCACAGA CTGCACCAGC CGCGAAGCCT 
GAGAAGGCCT CGGCCGGCGC CCGGCTTCCT GTGCAGGAGA AGACGAACCT GGCCGCCTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCGGAG ATCCTGGTCC GGAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCTTTCT CCTCGGACAT CAACAACATG 
CCCCTGCAGG AGCTGTCCAA CGCCCTCATG GCAGCTGAGC GCTTCAAGGA GCACCGGGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCA GCAGCCAGCA CGGCCAAACC CCCTCCTCTG 
CCTCGCTCCA ACACAGTGGC CTCTTTCTCC TCCCTGTACC AGTCCAGCTG CCAAGGACAG 
CTGCACAGGA GCGTTTCCTG GGCAGACTCC GCCGTGGTCA TGGAGGAGGG AAGTCCTGGC 
GAGGTTCCTG TGCCAGTGGA GCCCCCAGGA TTGGAGGACT TTGAGGCGGC GCTAGGCGTG 
GACAGGCGCA CAGACGCCTA CAGCAGGTCG TCCTCAACCT CCAGCCAGGA GGAGAAGTCA 
TTCCACGCAG AGGAGCTGGT TGCCGGGGGA ATCCCCATTG AACGAGCCGT CTCCTCGGAG 
GGTGGCCGGC CCTCTGTGGA CCTCTCCTTC CAGCCCTCAC AGCCCCTGAG CAAGTCTAGC 
TCCTCTCCTG AGCTGCAGAC CCTGCAGGAC ATCCTCGGGG ACCCTGGGGA CAAGGCTGAC 
GTGGGCCGGC TGAGCCCTGA GGTTAAGGCC CGTTCACAGT CAGGGATCCT GGACGGGGAA 
AGTGCTGCCT GGCCAGCCTC GGGCGAAGAC AGTCGGGGCC AGCCCGAGGG TCCCTTGCCT 
TCCAGCTCCC CCCGCTCACC CAGTGGCCTC CGGCCCCGCG GTTACACCAT CTCCGACTCG 
GCCCCATCAC GCAGGGGCAG GAGGGTAGAA AGGGATGCCT TTAAGAGCAG AGCCACAGCT 
TCCAATGCAG AGAAAGTGCC AGGCATCAAC CCCAGGTGGG CCTCTTGCTT CCGGGCGGGG 
CTCCTGATAC CATCCTGCAG GAACCTGGTG CCTCAGTTGC CCCAGGCCGA GCAGGCTGGG 
GAGGGTCCTC GCCTGTGCCC TAGGGCTGGC TGGAGCCCCT GGCAAGGCGG TGGAGTGGGA 
GATGGCCAGG TTCTGTGTTC CTCCCTGGGC TGTGGCTGCC CTGGCCAGGC CTTCACCTGG 
GTGCCCACTG TCTCCTCCCT GTGCAGTTTC GTGTTCCTGC AGCTCTACCA TTCGCCTTTC 
TTTGGCGACG AGTCAAACAA GCCAATCCTG CTGCCCAATG AGTCACAGTC CTTTGAGCGG 
TCGGTGCAGC TCCTCGACCA GATCCCATCA TACGACACCC ACAAGATCGC CGTCCTGTAT 
GTGGGAGAAG GCCAGAGCAA CAGTGAGCTC GCCATCCTGT CCAATGAGCA TGGCTCCTAC 
AGGTACACGG AGTTCCTGAC GGGCCTGGGC CGGCTCATCG AGCTCAAGGA CTGCCAGCCA 
GACAAGGTGT ACCTGGGCGG CCTGGACGTG TGTGGTGAGG ACGGCCAGTT CACCTACTGC 
TGGCACGACG ACATCATGCA AGCCGTCTTC CACATCGCCA CCCTGATGCC CACCAAGGAC 
GTGGACAAGC ATCGTTGCGA CAAGAAGCGC CACCTGGGCA ACGATTTCGT GTCCATTGTC 
TACAATGACT CTGGCGAGGA CTTCAAGCTC GGCACCATCA AGGGCCAGTT CAACTTTGTC 
CACGTGATCA TCACCCCGCT GGATTATGAG TGCAACCTGG TGTCCCTGCA GTGCAGGAAA 
GACATGGAGG GCCTTGTGGA CACCAGCGTG GCCAAGATCG TGTCTGACCG CAACCTGCCT 
TTTGTGGCCC GTCAGATGGC CCTGCATGCA AACATGGCCT CACAGGTGCA CCACAGCCGC 
TCCAACCCCA CCGACATCTA CCCCTCCAAG TGGATTGCCC GGCTCCGCCA CATCAAGAGA 
CTCCGCCAGC GGATCTGCGA GGAAGCCGCC TACTCCAACC CCAGCCTGCC TCTGGTGCAC 
CCCCCATCCC ATACCAAAGG GCCTGCACAG ACTCCAGCCG AGCCCACGCC TGGCTACGAG 
ATGGGCCAGC GGAAGCGCCT CATCTCCTCT GTGGAGGACT TCACCGAGTT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028697320.1
CDS	204..6140
Translation	MLRSGRGATCCSFSPPGRRGLLPTPPRSSPGSQAPVPRQHPGAQGVHAGLLRTTHPGGPRGPRHVTQGSPAFPLEGCLELQLPAGSVAALRAAPLPEVRVPLPAASGARGVRLSPRRGGPARGGAAREGCSGASWPTMAKPASKDSGLKEKFRILFGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLSNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIIQGQGERLGVLRALFFKVIKDYPSNEDLHERLDVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASNAEKVPGINPRWASCFRAGLLIPSCRNLVPQLPQAEQAGEGPRLCPRAGWSPWQGGGVGDGQVLCSSLGCGCPGQAFTWVPTVSSLCSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKGPAQTPAEPTPGYEMGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_028841487.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028841487.1

ORF Insert Sequence:

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAGGCTCCGT GGCGGCCCTC CGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
CCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAGCAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACCGCCGAA 
ATCCTGAGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTATATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCGTCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCGGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGCA GGATACAGAC GTCCCTCACC AGTGCCAGCC TGGGGTCTGC AGACGAGAAC 
TCCGTGGCCC AGGCTGACGA TAGCCTGAAA AGCCTCCATC TGGAGCTCAC GGAAACCTGT 
CTGGACATGA TGGCCCGATA CGTCTTCTCC AACTTCACAG CTGTCCCGAA GAGGTCTCCT 
GTGGGCGAGT TCCTCCTGGC GGGTGGCAGG ACCAAAACCT GGCTGGTTGG GAACAAGCTT 
GTCACTGTGA CGACGAGTGT GGGAACCGGG ACCCGGTCGT TACTAGGCCT GGACTCGGGG 
GAGCTGCAGT CCGGCCCGGA GTCGAGCTCC AGCCCCGGCA TGAATGTGAG ACAGACGAAG 
GAGGCGCCAG CCAAGCTGGA GTCCCAGGCT GGGCAGCAGG TGTCCCGTGG GGCCCGGGAC 
CGGGTCCGTT CTATGTCAGG GGGCCATGGT CTTCGAGTTG GCGCCCTGGA TGTGCCGGCC 
TCCCAGTTCC CGGGCAGTGC CGCTTCTCCA GGACCACAGA CTGCACCAGC CGCGAAGCCT 
GAGAAGGCCT CGGCCGGCGC CCGGCTTCCT GTGCAGGAGA AGACGAACCT GGCCGCCTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCGGAG ATCCTGGTCC GGAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCTTTCT CCTCGGACAT CAACAACATG 
CCCCTGCAGG AGCTGTCCAA CGCCCTCATG GCAGCTGAGC GCTTCAAGGA GCACCGGGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCA GCAGCCAGCA CGGCCAAACC CCCTCCTCTG 
CCTCGCTCCA ACACAGTGGC CTCTTTCTCC TCCCTGTACC AGTCCAGCTG CCAAGGACAG 
CTGCACAGGA GCGTTTCCTG GGCAGACTCC GCCGTGGTCA TGGAGGAGGG AAGTCCTGGC 
GAGGTTCCTG TGCCAGTGGA GCCCCCAGGA TTGGAGGACT TTGAGGCGGC GCTAGGCGTG 
GACAGGCGCA CAGACGCCTA CAGCAGGTCG TCCTCAACCT CCAGCCAGGA GGAGAAGTCA 
TTCCACGCAG AGGAGCTGGT TGCCGGGGGA ATCCCCATTG AACGAGCCGT CTCCTCGGAG 
GGTGGCCGGC CCTCTGTGGA CCTCTCCTTC CAGCCCTCAC AGCCCCTGAG CAAGTCTAGC 
TCCTCTCCTG AGCTGCAGAC CCTGCAGGAC ATCCTCGGGG ACCCTGGGGA CAAGGCTGAC 
GTGGGCCGGC TGAGCCCTGA GGTTAAGGCC CGTTCACAGT CAGGGATCCT GGACGGGGAA 
AGTGCTGCCT GGCCAGCCTC GGGCGAAGAC AGTCGGGGCC AGCCCGAGGG TCCCTTGCCT 
TCCAGCTCCC CCCGCTCACC CAGTGGCCTC CGGCCCCGCG GTTACACCAT CTCCGACTCG 
GCCCCATCAC GCAGGGGCAG GAGGGTAGAA AGGGATGCCT TTAAGAGCAG AGCCACAGCT 
TCCAATGCAG AGAAAGTGCC AGGCATCAAC CCCAGGTGGG CCTCTTGCTT CCGGGCGGGG 
CTCCTGATAC CATCCTGCAG GAACCTGGTG CCTCAGTTGC CCCAGGCCGA GCAGGCTGGG 
GAGGGTCCTC GCCTGTGCCC TAGGGCTGGC TGGAGCCCCT GGCAAGGCGG TGGAGTGGGA 
GATGGCCAGG TTCTGTGTTC CTCCCTGGGC TGTGGCTGCC CTGGCCAGGC CTTCACCTGG 
GTGCCCACTG TCTCCTCCCT GTGCAGTTTC GTGTTCCTGC AGCTCTACCA TTCGCCTTTC 
TTTGGCGACG AGTCAAACAA GCCAATCCTG CTGCCCAATG AGTCACAGTC CTTTGAGCGG 
TCGGTGCAGC TCCTCGACCA GATCCCATCA TACGACACCC ACAAGATCGC CGTCCTGTAT 
GTGGGAGAAG GCCAGAGCAA CAGTGAGCTC GCCATCCTGT CCAATGAGCA TGGCTCCTAC 
AGGTACACGG AGTTCCTGAC GGGCCTGGGC CGGCTCATCG AGCTCAAGGA CTGCCAGCCA 
GACAAGGTGT ACCTGGGCGG CCTGGACGTG TGTGGTGAGG ACGGCCAGTT CACCTACTGC 
TGGCACGACG ACATCATGCA AGCCGTCTTC CACATCGCCA CCCTGATGCC CACCAAGGAC 
GTGGACAAGC ATCGTTGCGA CAAGAAGCGC CACCTGGGCA ACGATTTCGT GTCCATTGTC 
TACAATGACT CTGGCGAGGA CTTCAAGCTC GGCACCATCA AGGGCCAGTT CAACTTTGTC 
CACGTGATCA TCACCCCGCT GGATTATGAG TGCAACCTGG TGTCCCTGCA GTGCAGGAAA 
GACATGGAGG GCCTTGTGGA CACCAGCGTG GCCAAGATCG TGTCTGACCG CAACCTGCCT 
TTTGTGGCCC GTCAGATGGC CCTGCATGCA AACATGGCCT CACAGGTGCA CCACAGCCGC 
TCCAACCCCA CCGACATCTA CCCCTCCAAG TGGATTGCCC GGCTCCGCCA CATCAAGAGA 
CTCCGCCAGC GGATCTGCGA GGAAGCCGCC TACTCCAACC CCAGCCTGCC TCTGGTGCAC 
CCCCCATCCC ATACCAAAGG GCCTGCACAG ACTCCAGCCG AGCCCACGCC TGGCTACGAG 
ATGGGCCAGC GGAAGCGCCT CATCTCCTCT GTGGAGGACT TCACCGAGTT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_028841489.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028697322.1 Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X6, mRNA.

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CloneID	OMb81168
Clone ID Related Accession (Same CDS sequence)	XM_028841489.1
Accession Version	XM_028841489.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAGGCTCCGT GGCGGCCCTC CGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
CCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAGCAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACCGCCGAA 
ATCCTGAGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTATATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCGTCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCGGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGGA TACAGACGTC CCTCACCAGT GCCAGCCTGG GGTCTGCAGA CGAGAACTCC 
GTGGCCCAGG CTGACGATAG CCTGAAAAGC CTCCATCTGG AGCTCACGGA AACCTGTCTG 
GACATGATGG CCCGATACGT CTTCTCCAAC TTCACAGCTG TCCCGAAGAG GTCTCCTGTG 
GGCGAGTTCC TCCTGGCGGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACTGTGACGA CGAGTGTGGG AACCGGGACC CGGTCGTTAC TAGGCCTGGA CTCGGGGGAG 
CTGCAGTCCG GCCCGGAGTC GAGCTCCAGC CCCGGCATGA ATGTGAGACA GACGAAGGAG 
GCGCCAGCCA AGCTGGAGTC CCAGGCTGGG CAGCAGGTGT CCCGTGGGGC CCGGGACCGG 
GTCCGTTCTA TGTCAGGGGG CCATGGTCTT CGAGTTGGCG CCCTGGATGT GCCGGCCTCC 
CAGTTCCCGG GCAGTGCCGC TTCTCCAGGA CCACAGACTG CACCAGCCGC GAAGCCTGAG 
AAGGCCTCGG CCGGCGCCCG GCTTCCTGTG CAGGAGAAGA CGAACCTGGC CGCCTACGTG 
CCCCTGCTGA CCCAGGGCTG GGCGGAGATC CTGGTCCGGA GGCCCACAGG GAACACCAGC 
TGGCTGATGA GCCTGGAGAA CCCGCTCAGC CCTTTCTCCT CGGACATCAA CAACATGCCC 
CTGCAGGAGC TGTCCAACGC CCTCATGGCA GCTGAGCGCT TCAAGGAGCA CCGGGACACA 
GCCCTGTACA AGTCGCTGTC GGTGCCAGCA GCCAGCACGG CCAAACCCCC TCCTCTGCCT 
CGCTCCAACA CAGTGGCCTC TTTCTCCTCC CTGTACCAGT CCAGCTGCCA AGGACAGCTG 
CACAGGAGCG TTTCCTGGGC AGACTCCGCC GTGGTCATGG AGGAGGGAAG TCCTGGCGAG 
GTTCCTGTGC CAGTGGAGCC CCCAGGATTG GAGGACTTTG AGGCGGCGCT AGGCGTGGAC 
AGGCGCACAG ACGCCTACAG CAGGTCGTCC TCAACCTCCA GCCAGGAGGA GAAGTCATTC 
CACGCAGAGG AGCTGGTTGC CGGGGGAATC CCCATTGAAC GAGCCGTCTC CTCGGAGGGT 
GGCCGGCCCT CTGTGGACCT CTCCTTCCAG CCCTCACAGC CCCTGAGCAA GTCTAGCTCC 
TCTCCTGAGC TGCAGACCCT GCAGGACATC CTCGGGGACC CTGGGGACAA GGCTGACGTG 
GGCCGGCTGA GCCCTGAGGT TAAGGCCCGT TCACAGTCAG GGATCCTGGA CGGGGAAAGT 
GCTGCCTGGC CAGCCTCGGG CGAAGACAGT CGGGGCCAGC CCGAGGGTCC CTTGCCTTCC 
AGCTCCCCCC GCTCACCCAG TGGCCTCCGG CCCCGCGGTT ACACCATCTC CGACTCGGCC 
CCATCACGCA GGGGCAGGAG GGTAGAAAGG GATGCCTTTA AGAGCAGAGC CACAGCTTCC 
AATGCAGAGA AAGTGCCAGG CATCAACCCC AGGTGGGCCT CTTGCTTCCG GGCGGGGCTC 
CTGATACCAT CCTGCAGGAA CCTGGTGCCT CAGTTGCCCC AGGCCGAGCA GGCTGGGGAG 
GGTCCTCGCC TGTGCCCTAG GGCTGGCTGG AGCCCCTGGC AAGGCGGTGG AGTGGGAGAT 
GGCCAGGTTC TGTGTTCCTC CCTGGGCTGT GGCTGCCCTG GCCAGGCCTT CACCTGGGTG 
CCCACTGTCT CCTCCCTGTG CAGTTTCGTG TTCCTGCAGC TCTACCATTC GCCTTTCTTT 
GGCGACGAGT CAAACAAGCC AATCCTGCTG CCCAATGAGT CACAGTCCTT TGAGCGGTCG 
GTGCAGCTCC TCGACCAGAT CCCATCATAC GACACCCACA AGATCGCCGT CCTGTATGTG 
GGAGAAGGCC AGAGCAACAG TGAGCTCGCC ATCCTGTCCA ATGAGCATGG CTCCTACAGG 
TACACGGAGT TCCTGACGGG CCTGGGCCGG CTCATCGAGC TCAAGGACTG CCAGCCAGAC 
AAGGTGTACC TGGGCGGCCT GGACGTGTGT GGTGAGGACG GCCAGTTCAC CTACTGCTGG 
CACGACGACA TCATGCAAGC CGTCTTCCAC ATCGCCACCC TGATGCCCAC CAAGGACGTG 
GACAAGCATC GTTGCGACAA GAAGCGCCAC CTGGGCAACG ATTTCGTGTC CATTGTCTAC 
AATGACTCTG GCGAGGACTT CAAGCTCGGC ACCATCAAGG GCCAGTTCAA CTTTGTCCAC 
GTGATCATCA CCCCGCTGGA TTATGAGTGC AACCTGGTGT CCCTGCAGTG CAGGAAAGAC 
ATGGAGGGCC TTGTGGACAC CAGCGTGGCC AAGATCGTGT CTGACCGCAA CCTGCCTTTT 
GTGGCCCGTC AGATGGCCCT GCATGCAAAC ATGGCCTCAC AGGTGCACCA CAGCCGCTCC 
AACCCCACCG ACATCTACCC CTCCAAGTGG ATTGCCCGGC TCCGCCACAT CAAGAGACTC 
CGCCAGCGGA TCTGCGAGGA AGCCGCCTAC TCCAACCCCA GCCTGCCTCT GGTGCACCCC 
CCATCCCATA CCAAAGGGCC TGCACAGACT CCAGCCGAGC CCACGCCTGG CTACGAGATG 
GGCCAGCGGA AGCGCCTCAT CTCCTCTGTG GAGGACTTCA CCGAGTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028697322.1
CDS	204..6137
Translation	MLRSGRGATCCSFSPPGRRGLLPTPPRSSPGSQAPVPRQHPGAQGVHAGLLRTTHPGGPRGPRHVTQGSPAFPLEGCLELQLPAGSVAALRAAPLPEVRVPLPAASGARGVRLSPRRGGPARGGAAREGCSGASWPTMAKPASKDSGLKEKFRILFGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLSNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIIQGQGERLGVLRALFFKVIKDYPSNEDLHERLDVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASNAEKVPGINPRWASCFRAGLLIPSCRNLVPQLPQAEQAGEGPRLCPRAGWSPWQGGGVGDGQVLCSSLGCGCPGQAFTWVPTVSSLCSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKGPAQTPAEPTPGYEMGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_028841489.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028841489.1

ORF Insert Sequence:

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAGGCTCCGT GGCGGCCCTC CGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
CCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAGCAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACCGCCGAA 
ATCCTGAGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTATATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCGTCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCGGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGGA TACAGACGTC CCTCACCAGT GCCAGCCTGG GGTCTGCAGA CGAGAACTCC 
GTGGCCCAGG CTGACGATAG CCTGAAAAGC CTCCATCTGG AGCTCACGGA AACCTGTCTG 
GACATGATGG CCCGATACGT CTTCTCCAAC TTCACAGCTG TCCCGAAGAG GTCTCCTGTG 
GGCGAGTTCC TCCTGGCGGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACTGTGACGA CGAGTGTGGG AACCGGGACC CGGTCGTTAC TAGGCCTGGA CTCGGGGGAG 
CTGCAGTCCG GCCCGGAGTC GAGCTCCAGC CCCGGCATGA ATGTGAGACA GACGAAGGAG 
GCGCCAGCCA AGCTGGAGTC CCAGGCTGGG CAGCAGGTGT CCCGTGGGGC CCGGGACCGG 
GTCCGTTCTA TGTCAGGGGG CCATGGTCTT CGAGTTGGCG CCCTGGATGT GCCGGCCTCC 
CAGTTCCCGG GCAGTGCCGC TTCTCCAGGA CCACAGACTG CACCAGCCGC GAAGCCTGAG 
AAGGCCTCGG CCGGCGCCCG GCTTCCTGTG CAGGAGAAGA CGAACCTGGC CGCCTACGTG 
CCCCTGCTGA CCCAGGGCTG GGCGGAGATC CTGGTCCGGA GGCCCACAGG GAACACCAGC 
TGGCTGATGA GCCTGGAGAA CCCGCTCAGC CCTTTCTCCT CGGACATCAA CAACATGCCC 
CTGCAGGAGC TGTCCAACGC CCTCATGGCA GCTGAGCGCT TCAAGGAGCA CCGGGACACA 
GCCCTGTACA AGTCGCTGTC GGTGCCAGCA GCCAGCACGG CCAAACCCCC TCCTCTGCCT 
CGCTCCAACA CAGTGGCCTC TTTCTCCTCC CTGTACCAGT CCAGCTGCCA AGGACAGCTG 
CACAGGAGCG TTTCCTGGGC AGACTCCGCC GTGGTCATGG AGGAGGGAAG TCCTGGCGAG 
GTTCCTGTGC CAGTGGAGCC CCCAGGATTG GAGGACTTTG AGGCGGCGCT AGGCGTGGAC 
AGGCGCACAG ACGCCTACAG CAGGTCGTCC TCAACCTCCA GCCAGGAGGA GAAGTCATTC 
CACGCAGAGG AGCTGGTTGC CGGGGGAATC CCCATTGAAC GAGCCGTCTC CTCGGAGGGT 
GGCCGGCCCT CTGTGGACCT CTCCTTCCAG CCCTCACAGC CCCTGAGCAA GTCTAGCTCC 
TCTCCTGAGC TGCAGACCCT GCAGGACATC CTCGGGGACC CTGGGGACAA GGCTGACGTG 
GGCCGGCTGA GCCCTGAGGT TAAGGCCCGT TCACAGTCAG GGATCCTGGA CGGGGAAAGT 
GCTGCCTGGC CAGCCTCGGG CGAAGACAGT CGGGGCCAGC CCGAGGGTCC CTTGCCTTCC 
AGCTCCCCCC GCTCACCCAG TGGCCTCCGG CCCCGCGGTT ACACCATCTC CGACTCGGCC 
CCATCACGCA GGGGCAGGAG GGTAGAAAGG GATGCCTTTA AGAGCAGAGC CACAGCTTCC 
AATGCAGAGA AAGTGCCAGG CATCAACCCC AGGTGGGCCT CTTGCTTCCG GGCGGGGCTC 
CTGATACCAT CCTGCAGGAA CCTGGTGCCT CAGTTGCCCC AGGCCGAGCA GGCTGGGGAG 
GGTCCTCGCC TGTGCCCTAG GGCTGGCTGG AGCCCCTGGC AAGGCGGTGG AGTGGGAGAT 
GGCCAGGTTC TGTGTTCCTC CCTGGGCTGT GGCTGCCCTG GCCAGGCCTT CACCTGGGTG 
CCCACTGTCT CCTCCCTGTG CAGTTTCGTG TTCCTGCAGC TCTACCATTC GCCTTTCTTT 
GGCGACGAGT CAAACAAGCC AATCCTGCTG CCCAATGAGT CACAGTCCTT TGAGCGGTCG 
GTGCAGCTCC TCGACCAGAT CCCATCATAC GACACCCACA AGATCGCCGT CCTGTATGTG 
GGAGAAGGCC AGAGCAACAG TGAGCTCGCC ATCCTGTCCA ATGAGCATGG CTCCTACAGG 
TACACGGAGT TCCTGACGGG CCTGGGCCGG CTCATCGAGC TCAAGGACTG CCAGCCAGAC 
AAGGTGTACC TGGGCGGCCT GGACGTGTGT GGTGAGGACG GCCAGTTCAC CTACTGCTGG 
CACGACGACA TCATGCAAGC CGTCTTCCAC ATCGCCACCC TGATGCCCAC CAAGGACGTG 
GACAAGCATC GTTGCGACAA GAAGCGCCAC CTGGGCAACG ATTTCGTGTC CATTGTCTAC 
AATGACTCTG GCGAGGACTT CAAGCTCGGC ACCATCAAGG GCCAGTTCAA CTTTGTCCAC 
GTGATCATCA CCCCGCTGGA TTATGAGTGC AACCTGGTGT CCCTGCAGTG CAGGAAAGAC 
ATGGAGGGCC TTGTGGACAC CAGCGTGGCC AAGATCGTGT CTGACCGCAA CCTGCCTTTT 
GTGGCCCGTC AGATGGCCCT GCATGCAAAC ATGGCCTCAC AGGTGCACCA CAGCCGCTCC 
AACCCCACCG ACATCTACCC CTCCAAGTGG ATTGCCCGGC TCCGCCACAT CAAGAGACTC 
CGCCAGCGGA TCTGCGAGGA AGCCGCCTAC TCCAACCCCA GCCTGCCTCT GGTGCACCCC 
CCATCCCATA CCAAAGGGCC TGCACAGACT CCAGCCGAGC CCACGCCTGG CTACGAGATG 
GGCCAGCGGA AGCGCCTCAT CTCCTCTGTG GAGGACTTCA CCGAGTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015125349.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014980835.2 Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X8, mRNA.

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CloneID	OMb81169
Clone ID Related Accession (Same CDS sequence)	XM_015125349.2
Accession Version	XM_015125349.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_015125349.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAGGCTCCGT GGCGGCCCTC CGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
CCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAGCAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACCGCCGAA 
ATCCTGAGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTATATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCGTCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCGGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGTC TGAGGATAGC CAGACCCCCC AAACAAGGCT TGAATAACTC TCCACCCGTG 
AAAGAATTCA AGGAGAGCTC TGCAGCCGAG GCCTTCCGGT GCCGCAGCAT CAGTGTGTCT 
GAACATGTGG TCCGCAGCAG GATACAGACG TCCCTCACCA GTGCCAGCCT GGGGTCTGCA 
GACGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA GCCTCCATCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCCCGATAC GTCTTCTCCA ACTTCACAGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTGGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGTGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGCAT GAATGTGAGA 
CAGACGAAGG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGTTC TATGTCAGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAT 
GTGCCGGCCT CCCAGTTCCC GGGCAGTGCC GCTTCTCCAG GACCACAGAC TGCACCAGCC 
GCGAAGCCTG AGAAGGCCTC GGCCGGCGCC CGGCTTCCTG TGCAGGAGAA GACGAACCTG 
GCCGCCTACG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CAGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCGCTG TCGGTGCCAG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCCG CCGTGGTCAT GGAGGAGGGA 
AGTCCTGGCG AGGTTCCTGT GCCAGTGGAG CCCCCAGGAT TGGAGGACTT TGAGGCGGCG 
CTAGGCGTGG ACAGGCGCAC AGACGCCTAC AGCAGGTCGT CCTCAACCTC CAGCCAGGAG 
GAGAAGTCAT TCCACGCAGA GGAGCTGGTT GCCGGGGGAA TCCCCATTGA ACGAGCCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCACA GCCCCTGAGC 
AAGTCTAGCT CCTCTCCTGA GCTGCAGACC CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCTGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GTTCACAGTC AGGGATCCTG 
GACGGGGAAA GTGCTGCCTG GCCAGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCACCC AGTGGCCTCC GGCCCCGCGG TTACACCATC 
TCCGACTCGG CCCCATCACG CAGGGGCAGG AGGGTAGAAA GGGATGCCTT TAAGAGCAGA 
GCCACAGCTT CCAATGCAGA GAAAGTGCCA GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CGCCTTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT GGGAGAAGGC CAGAGCAACA GTGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTCAAGGACT GCCAGCCAGA CAAGGTGTAC CTGGGCGGCC TGGACGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGACGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAT CGTTGCGACA AGAAGCGCCA CCTGGGCAAC 
GATTTCGTGT CCATTGTCTA CAATGACTCT GGCGAGGACT TCAAGCTCGG CACCATCAAG 
GGCCAGTTCA ACTTTGTCCA CGTGATCATC ACCCCGCTGG ATTATGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGG TGGGGCCAGG GTGGGGCCGG TGTCCCTGCA GTGCAGGAAA 
GACATGGAGG GCCTTGTGGA CACCAGCGTG GCCAAGATCG TGTCTGACCG CAACCTGCCT 
TTTGTGGCCC GTCAGATGGC CCTGCATGCA AACATGGCCT CACAGGTGCA CCACAGCCGC 
TCCAACCCCA CCGACATCTA CCCCTCCAAG TGGATTGCCC GGCTCCGCCA CATCAAGAGA 
CTCCGCCAGC GGATCTGCGA GGAAGCCGCC TACTCCAACC CCAGCCTGCC TCTGGTGCAC 
CCCCCATCCC ATACCAAAGG GCCTGCACAG ACTCCAGCCG AGCCCACGCC TGGCTACGAG 
ATGGGCCAGC GGAAGCGCCT CATCTCCTCT GTGGAGGACT TCACCGAGTT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014980835.2
CDS	204..6080
Translation	MLRSGRGATCCSFSPPGRRGLLPTPPRSSPGSQAPVPRQHPGAQGVHAGLLRTTHPGGPRGPRHVTQGSPAFPLEGCLELQLPAGSVAALRAAPLPEVRVPLPAASGARGVRLSPRRGGPARGGAAREGCSGASWPTMAKPASKDSGLKEKFRILFGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLSNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIIQGQGERLGVLRALFFKVIKDYPSNEDLHERLDVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKGGARVGPVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKGPAQTPAEPTPGYEMGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_015125349.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015125349.2

ORF Insert Sequence:

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAGGCTCCGT GGCGGCCCTC CGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
CCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAGCAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACCGCCGAA 
ATCCTGAGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTATATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCGTCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCGGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGTC TGAGGATAGC CAGACCCCCC AAACAAGGCT TGAATAACTC TCCACCCGTG 
AAAGAATTCA AGGAGAGCTC TGCAGCCGAG GCCTTCCGGT GCCGCAGCAT CAGTGTGTCT 
GAACATGTGG TCCGCAGCAG GATACAGACG TCCCTCACCA GTGCCAGCCT GGGGTCTGCA 
GACGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA GCCTCCATCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCCCGATAC GTCTTCTCCA ACTTCACAGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTGGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGTGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGCAT GAATGTGAGA 
CAGACGAAGG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGTTC TATGTCAGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAT 
GTGCCGGCCT CCCAGTTCCC GGGCAGTGCC GCTTCTCCAG GACCACAGAC TGCACCAGCC 
GCGAAGCCTG AGAAGGCCTC GGCCGGCGCC CGGCTTCCTG TGCAGGAGAA GACGAACCTG 
GCCGCCTACG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CAGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCGCTG TCGGTGCCAG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCCG CCGTGGTCAT GGAGGAGGGA 
AGTCCTGGCG AGGTTCCTGT GCCAGTGGAG CCCCCAGGAT TGGAGGACTT TGAGGCGGCG 
CTAGGCGTGG ACAGGCGCAC AGACGCCTAC AGCAGGTCGT CCTCAACCTC CAGCCAGGAG 
GAGAAGTCAT TCCACGCAGA GGAGCTGGTT GCCGGGGGAA TCCCCATTGA ACGAGCCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCACA GCCCCTGAGC 
AAGTCTAGCT CCTCTCCTGA GCTGCAGACC CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCTGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GTTCACAGTC AGGGATCCTG 
GACGGGGAAA GTGCTGCCTG GCCAGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCACCC AGTGGCCTCC GGCCCCGCGG TTACACCATC 
TCCGACTCGG CCCCATCACG CAGGGGCAGG AGGGTAGAAA GGGATGCCTT TAAGAGCAGA 
GCCACAGCTT CCAATGCAGA GAAAGTGCCA GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CGCCTTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT GGGAGAAGGC CAGAGCAACA GTGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTCAAGGACT GCCAGCCAGA CAAGGTGTAC CTGGGCGGCC TGGACGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGACGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAT CGTTGCGACA AGAAGCGCCA CCTGGGCAAC 
GATTTCGTGT CCATTGTCTA CAATGACTCT GGCGAGGACT TCAAGCTCGG CACCATCAAG 
GGCCAGTTCA ACTTTGTCCA CGTGATCATC ACCCCGCTGG ATTATGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGG TGGGGCCAGG GTGGGGCCGG TGTCCCTGCA GTGCAGGAAA 
GACATGGAGG GCCTTGTGGA CACCAGCGTG GCCAAGATCG TGTCTGACCG CAACCTGCCT 
TTTGTGGCCC GTCAGATGGC CCTGCATGCA AACATGGCCT CACAGGTGCA CCACAGCCGC 
TCCAACCCCA CCGACATCTA CCCCTCCAAG TGGATTGCCC GGCTCCGCCA CATCAAGAGA 
CTCCGCCAGC GGATCTGCGA GGAAGCCGCC TACTCCAACC CCAGCCTGCC TCTGGTGCAC 
CCCCCATCCC ATACCAAAGG GCCTGCACAG ACTCCAGCCG AGCCCACGCC TGGCTACGAG 
ATGGGCCAGC GGAAGCGCCT CATCTCCTCT GTGGAGGACT TCACCGAGTT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_001083256.4 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_001083256.3 Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X9, mRNA.

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CloneID	OMb81170
Clone ID Related Accession (Same CDS sequence)	XM_001083256.4
Accession Version	XM_001083256.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_001083256.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAGGCTCCGT GGCGGCCCTC CGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
CCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAGCAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACCGCCGAA 
ATCCTGAGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTATATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCGTCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCGGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGTC TGAGGATAGC CAGACCCCCC AAACAAGGCT TGAATAACTC TCCACCCGTG 
AAAGAATTCA AGGAGAGCTC TGCAGCCGAG GCCTTCCGGT GCCGCAGCAT CAGTGTGTCT 
GAACATGTGG TCCGCAGCAG GATACAGACG TCCCTCACCA GTGCCAGCCT GGGGTCTGCA 
GACGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA GCCTCCATCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCCCGATAC GTCTTCTCCA ACTTCACAGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTGGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGTGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGCAT GAATGTGAGA 
CAGACGAAGG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGTTC TATGTCAGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAT 
GTGCCGGCCT CCCAGTTCCC GGGCAGTGCC GCTTCTCCAG GACCACAGAC TGCACCAGCC 
GCGAAGCCTG AGAAGGCCTC GGCCGGCGCC CGGCTTCCTG TGCAGGAGAA GACGAACCTG 
GCCGCCTACG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CAGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCGCTG TCGGTGCCAG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCCG CCGTGGTCAT GGAGGAGGGA 
AGTCCTGGCG AGGTTCCTGT GCCAGTGGAG CCCCCAGGAT TGGAGGACTT TGAGGCGGCG 
CTAGGCGTGG ACAGGCGCAC AGACGCCTAC AGCAGGTCGT CCTCAACCTC CAGCCAGGAG 
GAGAAGTCAT TCCACGCAGA GGAGCTGGTT GCCGGGGGAA TCCCCATTGA ACGAGCCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCACA GCCCCTGAGC 
AAGTCTAGCT CCTCTCCTGA GCTGCAGACC CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCTGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GTTCACAGTC AGGGATCCTG 
GACGGGGAAA GTGCTGCCTG GCCAGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCACCC AGTGGCCTCC GGCCCCGCGG TTACACCATC 
TCCGACTCGG CCCCATCACG CAGGGGCAGG AGGGTAGAAA GGGATGCCTT TAAGAGCAGA 
GCCACAGCTT CCAATGCAGA GAAAGTGCCA GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CGCCTTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT GGGAGAAGGC CAGAGCAACA GTGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTCAAGGACT GCCAGCCAGA CAAGGTGTAC CTGGGCGGCC TGGACGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGACGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAT CGTTGCGACA AGAAGCGCCA CCTGGGCAAC 
GATTTCGTGT CCATTGTCTA CAATGACTCT GGCGAGGACT TCAAGCTCGG CACCATCAAG 
GGCCAGTTCA ACTTTGTCCA CGTGATCATC ACCCCGCTGG ATTATGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACCTGCCTTT TGTGGCCCGT CAGATGGCCC TGCATGCAAA CATGGCCTCA 
CAGGTGCACC ACAGCCGCTC CAACCCCACC GACATCTACC CCTCCAAGTG GATTGCCCGG 
CTCCGCCACA TCAAGAGACT CCGCCAGCGG ATCTGCGAGG AAGCCGCCTA CTCCAACCCC 
AGCCTGCCTC TGGTGCACCC CCCATCCCAT ACCAAAGGGC CTGCACAGAC TCCAGCCGAG 
CCCACGCCTG GCTACGAGAT GGGCCAGCGG AAGCGCCTCA TCTCCTCTGT GGAGGACTTC 
ACCGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001083256.3
CDS	204..6038
Translation	MLRSGRGATCCSFSPPGRRGLLPTPPRSSPGSQAPVPRQHPGAQGVHAGLLRTTHPGGPRGPRHVTQGSPAFPLEGCLELQLPAGSVAALRAAPLPEVRVPLPAASGARGVRLSPRRGGPARGGAAREGCSGASWPTMAKPASKDSGLKEKFRILFGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLSNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIIQGQGERLGVLRALFFKVIKDYPSNEDLHERLDVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKGPAQTPAEPTPGYEMGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_001083256.4
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001083256.4

ORF Insert Sequence:

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAGGCTCCGT GGCGGCCCTC CGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
CCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAGCAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACCGCCGAA 
ATCCTGAGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTATATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCGTCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCGGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGTC TGAGGATAGC CAGACCCCCC AAACAAGGCT TGAATAACTC TCCACCCGTG 
AAAGAATTCA AGGAGAGCTC TGCAGCCGAG GCCTTCCGGT GCCGCAGCAT CAGTGTGTCT 
GAACATGTGG TCCGCAGCAG GATACAGACG TCCCTCACCA GTGCCAGCCT GGGGTCTGCA 
GACGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA GCCTCCATCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCCCGATAC GTCTTCTCCA ACTTCACAGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTGGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGTGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGCAT GAATGTGAGA 
CAGACGAAGG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGTTC TATGTCAGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAT 
GTGCCGGCCT CCCAGTTCCC GGGCAGTGCC GCTTCTCCAG GACCACAGAC TGCACCAGCC 
GCGAAGCCTG AGAAGGCCTC GGCCGGCGCC CGGCTTCCTG TGCAGGAGAA GACGAACCTG 
GCCGCCTACG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CAGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCGCTG TCGGTGCCAG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCCG CCGTGGTCAT GGAGGAGGGA 
AGTCCTGGCG AGGTTCCTGT GCCAGTGGAG CCCCCAGGAT TGGAGGACTT TGAGGCGGCG 
CTAGGCGTGG ACAGGCGCAC AGACGCCTAC AGCAGGTCGT CCTCAACCTC CAGCCAGGAG 
GAGAAGTCAT TCCACGCAGA GGAGCTGGTT GCCGGGGGAA TCCCCATTGA ACGAGCCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCACA GCCCCTGAGC 
AAGTCTAGCT CCTCTCCTGA GCTGCAGACC CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCTGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GTTCACAGTC AGGGATCCTG 
GACGGGGAAA GTGCTGCCTG GCCAGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCACCC AGTGGCCTCC GGCCCCGCGG TTACACCATC 
TCCGACTCGG CCCCATCACG CAGGGGCAGG AGGGTAGAAA GGGATGCCTT TAAGAGCAGA 
GCCACAGCTT CCAATGCAGA GAAAGTGCCA GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CGCCTTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT GGGAGAAGGC CAGAGCAACA GTGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTCAAGGACT GCCAGCCAGA CAAGGTGTAC CTGGGCGGCC TGGACGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGACGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAT CGTTGCGACA AGAAGCGCCA CCTGGGCAAC 
GATTTCGTGT CCATTGTCTA CAATGACTCT GGCGAGGACT TCAAGCTCGG CACCATCAAG 
GGCCAGTTCA ACTTTGTCCA CGTGATCATC ACCCCGCTGG ATTATGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACCTGCCTTT TGTGGCCCGT CAGATGGCCC TGCATGCAAA CATGGCCTCA 
CAGGTGCACC ACAGCCGCTC CAACCCCACC GACATCTACC CCTCCAAGTG GATTGCCCGG 
CTCCGCCACA TCAAGAGACT CCGCCAGCGG ATCTGCGAGG AAGCCGCCTA CTCCAACCCC 
AGCCTGCCTC TGGTGCACCC CCCATCCCAT ACCAAAGGGC CTGCACAGAC TCCAGCCGAG 
CCCACGCCTG GCTACGAGAT GGGCCAGCGG AAGCGCCTCA TCTCCTCTGT GGAGGACTTC 
ACCGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015125352.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014980838.2 Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X10, mRNA.

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CloneID	OMb81171
Clone ID Related Accession (Same CDS sequence)	XM_015125352.2
Accession Version	XM_015125352.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_015125352.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAGGCTCCGT GGCGGCCCTC CGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
CCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAGCAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACCGCCGAA 
ATCCTGAGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTATATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCGTCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCGGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGTC TGAGGATAGC CAGACCCCCC AAACAAGGCT TGAATAACTC TCCACCCGTG 
AAAGAATTCA AGGAGAGCTC TGCAGCCGAG GCCTTCCGGT GCCGCAGCAT CAGTGTGTCT 
GAACATGTGG TCCGCAGCAG GATACAGACG TCCCTCACCA GTGCCAGCCT GGGGTCTGCA 
GACGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA GCCTCCATCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCCCGATAC GTCTTCTCCA ACTTCACAGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTGGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGTGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGCAT GAATGTGAGA 
CAGACGAAGG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGTTC TATGTCAGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAT 
GTGCCGGCCT CCCAGTTCCC GGGCAGTGCC GCTTCTCCAG GACCACAGAC TGCACCAGCC 
GCGAAGCCTG AGAAGGCCTC GGCCGGCGCC CGGCTTCCTG TGCAGGAGAA GACGAACCTG 
GCCGCCTACG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CAGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCGCTG TCGGTGCCAG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCCG CCGTGGTCAT GGAGGAGGGA 
AGTCCTGGCG AGGTTCCTGT GCCAGTGGAG CCCCCAGGAT TGGAGGACTT TGAGGCGGCG 
CTAGGCGTGG ACAGGCGCAC AGACGCCTAC AGCAGGTCGT CCTCAACCTC CAGCCAGGAG 
GAGAAGTCAT TCCACGCAGA GGAGCTGGTT GCCGGGGGAA TCCCCATTGA ACGAGCCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCACA GCCCCTGAGC 
AAGTCTAGCT CCTCTCCTGA GCTGCAGACC CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCTGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GTTCACAGTC AGGGATCCTG 
GACGGGGAAA GTGCTGCCTG GCCAGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCACCC AGTGGCCTCC GGCCCCGCGG TTACACCATC 
TCCGACTCGG CCCCATCACG CAGGGGCAGG AGGGTAGAAA GGGATGCCTT TAAGAGCAGA 
GCCACAGCTT CCAATGCAGA GAAAGTGCCA GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CGCCTTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT GGGAGAAGGC CAGAGCAACA GTGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTCAAGGACT GCCAGCCAGA CAAGGTGTAC CTGGGCGGCC TGGACGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGACGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAT CGTTGCGACA AGAAGCGCCA CCTGGGCAAC 
GATTTCGTGT CCATTGTCTA CAATGACTCT GGCGAGGACT TCAAGCTCGG CACCATCAAG 
ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAGATCGT GTCTGACCGC AACCTGCCTT 
TTGTGGCCCG TCAGATGGCC CTGCATGCAA ACATGGCCTC ACAGGTGCAC CACAGCCGCT 
CCAACCCCAC CGACATCTAC CCCTCCAAGT GGATTGCCCG GCTCCGCCAC ATCAAGAGAC 
TCCGCCAGCG GATCTGCGAG GAAGCCGCCT ACTCCAACCC CAGCCTGCCT CTGGTGCACC 
CCCCATCCCA TACCAAAGGG CCTGCACAGA CTCCAGCCGA GCCCACGCCT GGCTACGAGA 
TGGGCCAGCG GAAGCGCCTC ATCTCCTCTG TGGAGGACTT CACCGAGTTC GTGTGAGGCT 
GGGGCCCTCC CTGCTGCGCC CGCCCTGGAC GCTATTTTGT GTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014980838.2
CDS	204..6011
Translation	MLRSGRGATCCSFSPPGRRGLLPTPPRSSPGSQAPVPRQHPGAQGVHAGLLRTTHPGGPRGPRHVTQGSPAFPLEGCLELQLPAGSVAALRAAPLPEVRVPLPAASGARGVRLSPRRGGPARGGAAREGCSGASWPTMAKPASKDSGLKEKFRILFGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLSNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIIQGQGERLGVLRALFFKVIKDYPSNEDLHERLDVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKTWRALWTPAWPRSCLTATCLLWPVRWPCMQTWPHRCTTAAPTPPTSTPPSGLPGSATSRDSASGSARKPPTPTPACLWCTPHPIPKGLHRLQPSPRLATRWASGSASSPLWRTSPSSCEAGALPAAPALDAILCQ

Target ORF information:

RefSeq Version	XM_015125352.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015125352.2

ORF Insert Sequence:

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAGGCTCCGT GGCGGCCCTC CGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
CCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAGCAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACCGCCGAA 
ATCCTGAGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTATATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCGTCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCGGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGTC TGAGGATAGC CAGACCCCCC AAACAAGGCT TGAATAACTC TCCACCCGTG 
AAAGAATTCA AGGAGAGCTC TGCAGCCGAG GCCTTCCGGT GCCGCAGCAT CAGTGTGTCT 
GAACATGTGG TCCGCAGCAG GATACAGACG TCCCTCACCA GTGCCAGCCT GGGGTCTGCA 
GACGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA GCCTCCATCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCCCGATAC GTCTTCTCCA ACTTCACAGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTGGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGTGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGCAT GAATGTGAGA 
CAGACGAAGG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGTTC TATGTCAGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAT 
GTGCCGGCCT CCCAGTTCCC GGGCAGTGCC GCTTCTCCAG GACCACAGAC TGCACCAGCC 
GCGAAGCCTG AGAAGGCCTC GGCCGGCGCC CGGCTTCCTG TGCAGGAGAA GACGAACCTG 
GCCGCCTACG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CAGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCGCTG TCGGTGCCAG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCCG CCGTGGTCAT GGAGGAGGGA 
AGTCCTGGCG AGGTTCCTGT GCCAGTGGAG CCCCCAGGAT TGGAGGACTT TGAGGCGGCG 
CTAGGCGTGG ACAGGCGCAC AGACGCCTAC AGCAGGTCGT CCTCAACCTC CAGCCAGGAG 
GAGAAGTCAT TCCACGCAGA GGAGCTGGTT GCCGGGGGAA TCCCCATTGA ACGAGCCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCACA GCCCCTGAGC 
AAGTCTAGCT CCTCTCCTGA GCTGCAGACC CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCTGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GTTCACAGTC AGGGATCCTG 
GACGGGGAAA GTGCTGCCTG GCCAGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCACCC AGTGGCCTCC GGCCCCGCGG TTACACCATC 
TCCGACTCGG CCCCATCACG CAGGGGCAGG AGGGTAGAAA GGGATGCCTT TAAGAGCAGA 
GCCACAGCTT CCAATGCAGA GAAAGTGCCA GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CGCCTTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT GGGAGAAGGC CAGAGCAACA GTGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTCAAGGACT GCCAGCCAGA CAAGGTGTAC CTGGGCGGCC TGGACGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGACGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAT CGTTGCGACA AGAAGCGCCA CCTGGGCAAC 
GATTTCGTGT CCATTGTCTA CAATGACTCT GGCGAGGACT TCAAGCTCGG CACCATCAAG 
ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAGATCGT GTCTGACCGC AACCTGCCTT 
TTGTGGCCCG TCAGATGGCC CTGCATGCAA ACATGGCCTC ACAGGTGCAC CACAGCCGCT 
CCAACCCCAC CGACATCTAC CCCTCCAAGT GGATTGCCCG GCTCCGCCAC ATCAAGAGAC 
TCCGCCAGCG GATCTGCGAG GAAGCCGCCT ACTCCAACCC CAGCCTGCCT CTGGTGCACC 
CCCCATCCCA TACCAAAGGG CCTGCACAGA CTCCAGCCGA GCCCACGCCT GGCTACGAGA 
TGGGCCAGCG GAAGCGCCTC ATCTCCTCTG TGGAGGACTT CACCGAGTTC GTGTGAGGCT 
GGGGCCCTCC CTGCTGCGCC CGCCCTGGAC GCTATTTTGT GTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_001083508.4 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_001083508.3 Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X11, mRNA.

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CloneID	OMb81172
Clone ID Related Accession (Same CDS sequence)	XM_001083508.4
Accession Version	XM_001083508.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_001083508.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAGGCTCCGT GGCGGCCCTC CGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
CCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAGCAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACCGCCGAA 
ATCCTGAGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTATATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCGTCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCGGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGTC TGAGGATAGC CAGACCCCCC AAACAAGGCT TGAATAACTC TCCACCCGTG 
AAAGAATTCA AGGAGAGCTC TGCAGCCGAG GCCTTCCGGT GCCGCAGCAT CAGTGTGTCT 
GAACATGTGG TCCGCAGCAG GATACAGACG TCCCTCACCA GTGCCAGCCT GGGGTCTGCA 
GACGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA GCCTCCATCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCCCGATAC GTCTTCTCCA ACTTCACAGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTGGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGTGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGCAT GAATGTGAGA 
CAGACGAAGG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGTTC TATGTCAGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAT 
GTGCCGGCCT CCCAGTTCCC GGGCAGTGCC GCTTCTCCAG GACCACAGAC TGCACCAGCC 
GCGAAGCCTG AGAAGGCCTC GGCCGGCGCC CGGCTTCCTG TGCAGGAGAA GACGAACCTG 
GCCGCCTACG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CAGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCGCTG TCGGTGCCAG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGACTCC GCCGTGGTCA TGGAGGAGGG AAGTCCTGGC 
GAGGTTCCTG TGCCAGTGGA GCCCCCAGGA TTGGAGGACT TTGAGGCGGC GCTAGGCGTG 
GACAGGCGCA CAGACGCCTA CAGCAGGTCG TCCTCAACCT CCAGCCAGGA GGAGAAGTCA 
TTCCACGCAG AGGAGCTGGT TGCCGGGGGA ATCCCCATTG AACGAGCCGT CTCCTCGGAG 
GGTGGCCGGC CCTCTGTGGA CCTCTCCTTC CAGCCCTCAC AGCCCCTGAG CAAGTCTAGC 
TCCTCTCCTG AGCTGCAGAC CCTGCAGGAC ATCCTCGGGG ACCCTGGGGA CAAGGCTGAC 
GTGGGCCGGC TGAGCCCTGA GGTTAAGGCC CGTTCACAGT CAGGGATCCT GGACGGGGAA 
AGTGCTGCCT GGCCAGCCTC GGGCGAAGAC AGTCGGGGCC AGCCCGAGGG TCCCTTGCCT 
TCCAGCTCCC CCCGCTCACC CAGTGGCCTC CGGCCCCGCG GTTACACCAT CTCCGACTCG 
GCCCCATCAC GCAGGGGCAG GAGGGTAGAA AGGGATGCCT TTAAGAGCAG AGCCACAGCT 
TCCAATGCAG AGAAAGTGCC AGGCATCAAC CCCAGTTTCG TGTTCCTGCA GCTCTACCAT 
TCGCCTTTCT TTGGCGACGA GTCAAACAAG CCAATCCTGC TGCCCAATGA GTCACAGTCC 
TTTGAGCGGT CGGTGCAGCT CCTCGACCAG ATCCCATCAT ACGACACCCA CAAGATCGCC 
GTCCTGTATG TGGGAGAAGG CCAGAGCAAC AGTGAGCTCG CCATCCTGTC CAATGAGCAT 
GGCTCCTACA GGTACACGGA GTTCCTGACG GGCCTGGGCC GGCTCATCGA GCTCAAGGAC 
TGCCAGCCAG ACAAGGTGTA CCTGGGCGGC CTGGACGTGT GTGGTGAGGA CGGCCAGTTC 
ACCTACTGCT GGCACGACGA CATCATGCAA GCCGTCTTCC ACATCGCCAC CCTGATGCCC 
ACCAAGGACG TGGACAAGCA TCGTTGCGAC AAGAAGCGCC ACCTGGGCAA CGATTTCGTG 
TCCATTGTCT ACAATGACTC TGGCGAGGAC TTCAAGCTCG GCACCATCAA GGGCCAGTTC 
AACTTTGTCC ACGTGATCAT CACCCCGCTG GATTATGAGT GCAACCTGGT GTCCCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAGATCGT GTCTGACCGC 
AACCTGCCTT TTGTGGCCCG TCAGATGGCC CTGCATGCAA ACATGGCCTC ACAGGTGCAC 
CACAGCCGCT CCAACCCCAC CGACATCTAC CCCTCCAAGT GGATTGCCCG GCTCCGCCAC 
ATCAAGAGAC TCCGCCAGCG GATCTGCGAG GAAGCCGCCT ACTCCAACCC CAGCCTGCCT 
CTGGTGCACC CCCCATCCCA TACCAAAGGG CCTGCACAGA CTCCAGCCGA GCCCACGCCT 
GGCTACGAGA TGGGCCAGCG GAAGCGCCTC ATCTCCTCTG TGGAGGACTT CACCGAGTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001083508.3
CDS	204..5969
Translation	MLRSGRGATCCSFSPPGRRGLLPTPPRSSPGSQAPVPRQHPGAQGVHAGLLRTTHPGGPRGPRHVTQGSPAFPLEGCLELQLPAGSVAALRAAPLPEVRVPLPAASGARGVRLSPRRGGPARGGAAREGCSGASWPTMAKPASKDSGLKEKFRILFGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLSNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIIQGQGERLGVLRALFFKVIKDYPSNEDLHERLDVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTDSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKGPAQTPAEPTPGYEMGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_001083508.4
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001083508.4

ORF Insert Sequence:

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAGGCTCCGT GGCGGCCCTC CGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
CCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAGCAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACCGCCGAA 
ATCCTGAGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTATATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCGTCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCGGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGTC TGAGGATAGC CAGACCCCCC AAACAAGGCT TGAATAACTC TCCACCCGTG 
AAAGAATTCA AGGAGAGCTC TGCAGCCGAG GCCTTCCGGT GCCGCAGCAT CAGTGTGTCT 
GAACATGTGG TCCGCAGCAG GATACAGACG TCCCTCACCA GTGCCAGCCT GGGGTCTGCA 
GACGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA GCCTCCATCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCCCGATAC GTCTTCTCCA ACTTCACAGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTGGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGTGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGCAT GAATGTGAGA 
CAGACGAAGG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGTTC TATGTCAGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAT 
GTGCCGGCCT CCCAGTTCCC GGGCAGTGCC GCTTCTCCAG GACCACAGAC TGCACCAGCC 
GCGAAGCCTG AGAAGGCCTC GGCCGGCGCC CGGCTTCCTG TGCAGGAGAA GACGAACCTG 
GCCGCCTACG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CAGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCGCTG TCGGTGCCAG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGACTCC GCCGTGGTCA TGGAGGAGGG AAGTCCTGGC 
GAGGTTCCTG TGCCAGTGGA GCCCCCAGGA TTGGAGGACT TTGAGGCGGC GCTAGGCGTG 
GACAGGCGCA CAGACGCCTA CAGCAGGTCG TCCTCAACCT CCAGCCAGGA GGAGAAGTCA 
TTCCACGCAG AGGAGCTGGT TGCCGGGGGA ATCCCCATTG AACGAGCCGT CTCCTCGGAG 
GGTGGCCGGC CCTCTGTGGA CCTCTCCTTC CAGCCCTCAC AGCCCCTGAG CAAGTCTAGC 
TCCTCTCCTG AGCTGCAGAC CCTGCAGGAC ATCCTCGGGG ACCCTGGGGA CAAGGCTGAC 
GTGGGCCGGC TGAGCCCTGA GGTTAAGGCC CGTTCACAGT CAGGGATCCT GGACGGGGAA 
AGTGCTGCCT GGCCAGCCTC GGGCGAAGAC AGTCGGGGCC AGCCCGAGGG TCCCTTGCCT 
TCCAGCTCCC CCCGCTCACC CAGTGGCCTC CGGCCCCGCG GTTACACCAT CTCCGACTCG 
GCCCCATCAC GCAGGGGCAG GAGGGTAGAA AGGGATGCCT TTAAGAGCAG AGCCACAGCT 
TCCAATGCAG AGAAAGTGCC AGGCATCAAC CCCAGTTTCG TGTTCCTGCA GCTCTACCAT 
TCGCCTTTCT TTGGCGACGA GTCAAACAAG CCAATCCTGC TGCCCAATGA GTCACAGTCC 
TTTGAGCGGT CGGTGCAGCT CCTCGACCAG ATCCCATCAT ACGACACCCA CAAGATCGCC 
GTCCTGTATG TGGGAGAAGG CCAGAGCAAC AGTGAGCTCG CCATCCTGTC CAATGAGCAT 
GGCTCCTACA GGTACACGGA GTTCCTGACG GGCCTGGGCC GGCTCATCGA GCTCAAGGAC 
TGCCAGCCAG ACAAGGTGTA CCTGGGCGGC CTGGACGTGT GTGGTGAGGA CGGCCAGTTC 
ACCTACTGCT GGCACGACGA CATCATGCAA GCCGTCTTCC ACATCGCCAC CCTGATGCCC 
ACCAAGGACG TGGACAAGCA TCGTTGCGAC AAGAAGCGCC ACCTGGGCAA CGATTTCGTG 
TCCATTGTCT ACAATGACTC TGGCGAGGAC TTCAAGCTCG GCACCATCAA GGGCCAGTTC 
AACTTTGTCC ACGTGATCAT CACCCCGCTG GATTATGAGT GCAACCTGGT GTCCCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAGATCGT GTCTGACCGC 
AACCTGCCTT TTGTGGCCCG TCAGATGGCC CTGCATGCAA ACATGGCCTC ACAGGTGCAC 
CACAGCCGCT CCAACCCCAC CGACATCTAC CCCTCCAAGT GGATTGCCCG GCTCCGCCAC 
ATCAAGAGAC TCCGCCAGCG GATCTGCGAG GAAGCCGCCT ACTCCAACCC CAGCCTGCCT 
CTGGTGCACC CCCCATCCCA TACCAAAGGG CCTGCACAGA CTCCAGCCGA GCCCACGCCT 
GGCTACGAGA TGGGCCAGCG GAAGCGCCTC ATCTCCTCTG TGGAGGACTT CACCGAGTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_028841490.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028697323.1 Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X12, mRNA.

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CloneID	OMb81173
Clone ID Related Accession (Same CDS sequence)	XM_028841490.1
Accession Version	XM_028841490.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAGGCTCCGT GGCGGCCCTC CGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
CCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAGCAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACCGCCGAA 
ATCCTGAGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTATATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCGTCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCGGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGCA GGATACAGAC GTCCCTCACC AGTGCCAGCC TGGGGTCTGC AGACGAGAAC 
TCCGTGGCCC AGGCTGACGA TAGCCTGAAA AGCCTCCATC TGGAGCTCAC GGAAACCTGT 
CTGGACATGA TGGCCCGATA CGTCTTCTCC AACTTCACAG CTGTCCCGAA GAGGTCTCCT 
GTGGGCGAGT TCCTCCTGGC GGGTGGCAGG ACCAAAACCT GGCTGGTTGG GAACAAGCTT 
GTCACTGTGA CGACGAGTGT GGGAACCGGG ACCCGGTCGT TACTAGGCCT GGACTCGGGG 
GAGCTGCAGT CCGGCCCGGA GTCGAGCTCC AGCCCCGGCA TGAATGTGAG ACAGACGAAG 
GAGGCGCCAG CCAAGCTGGA GTCCCAGGCT GGGCAGCAGG TGTCCCGTGG GGCCCGGGAC 
CGGGTCCGTT CTATGTCAGG GGGCCATGGT CTTCGAGTTG GCGCCCTGGA TGTGCCGGCC 
TCCCAGTTCC CGGGCAGTGC CGCTTCTCCA GGACCACAGA CTGCACCAGC CGCGAAGCCT 
GAGAAGGCCT CGGCCGGCGC CCGGCTTCCT GTGCAGGAGA AGACGAACCT GGCCGCCTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCGGAG ATCCTGGTCC GGAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCTTTCT CCTCGGACAT CAACAACATG 
CCCCTGCAGG AGCTGTCCAA CGCCCTCATG GCAGCTGAGC GCTTCAAGGA GCACCGGGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCA GCAGCCAGCA CGGCCAAACC CCCTCCTCTG 
CCTCGCTCCA ACACAGTGGC CTCTTTCTCC TCCCTGTACC AGTCCAGCTG CCAAGGACAG 
CTGCACAGGA GCGTTTCCTG GGCAGACTCC GCCGTGGTCA TGGAGGAGGG AAGTCCTGGC 
GAGGTTCCTG TGCCAGTGGA GCCCCCAGGA TTGGAGGACT TTGAGGCGGC GCTAGGCGTG 
GACAGGCGCA CAGACGCCTA CAGCAGGTCG TCCTCAACCT CCAGCCAGGA GGAGAAGTCA 
TTCCACGCAG AGGAGCTGGT TGCCGGGGGA ATCCCCATTG AACGAGCCGT CTCCTCGGAG 
GGTGGCCGGC CCTCTGTGGA CCTCTCCTTC CAGCCCTCAC AGCCCCTGAG CAAGTCTAGC 
TCCTCTCCTG AGCTGCAGAC CCTGCAGGAC ATCCTCGGGG ACCCTGGGGA CAAGGCTGAC 
GTGGGCCGGC TGAGCCCTGA GGTTAAGGCC CGTTCACAGT CAGGGATCCT GGACGGGGAA 
AGTGCTGCCT GGCCAGCCTC GGGCGAAGAC AGTCGGGGCC AGCCCGAGGG TCCCTTGCCT 
TCCAGCTCCC CCCGCTCACC CAGTGGCCTC CGGCCCCGCG GTTACACCAT CTCCGACTCG 
GCCCCATCAC GCAGGGGCAG GAGGGTAGAA AGGGATGCCT TTAAGAGCAG AGCCACAGCT 
TCCAATGCAG AGAAAGTGCC AGGCATCAAC CCCAGTTTCG TGTTCCTGCA GCTCTACCAT 
TCGCCTTTCT TTGGCGACGA GTCAAACAAG CCAATCCTGC TGCCCAATGA GTCACAGTCC 
TTTGAGCGGT CGGTGCAGCT CCTCGACCAG ATCCCATCAT ACGACACCCA CAAGATCGCC 
GTCCTGTATG TGGGAGAAGG CCAGAGCAAC AGTGAGCTCG CCATCCTGTC CAATGAGCAT 
GGCTCCTACA GGTACACGGA GTTCCTGACG GGCCTGGGCC GGCTCATCGA GCTCAAGGAC 
TGCCAGCCAG ACAAGGTGTA CCTGGGCGGC CTGGACGTGT GTGGTGAGGA CGGCCAGTTC 
ACCTACTGCT GGCACGACGA CATCATGCAA GCCGTCTTCC ACATCGCCAC CCTGATGCCC 
ACCAAGGACG TGGACAAGCA TCGTTGCGAC AAGAAGCGCC ACCTGGGCAA CGATTTCGTG 
TCCATTGTCT ACAATGACTC TGGCGAGGAC TTCAAGCTCG GCACCATCAA GGGCCAGTTC 
AACTTTGTCC ACGTGATCAT CACCCCGCTG GATTATGAGT GCAACCTGGT GTCCCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAGATCGT GTCTGACCGC 
AACCTGCCTT TTGTGGCCCG TCAGATGGCC CTGCATGCAA ACATGGCCTC ACAGGTGCAC 
CACAGCCGCT CCAACCCCAC CGACATCTAC CCCTCCAAGT GGATTGCCCG GCTCCGCCAC 
ATCAAGAGAC TCCGCCAGCG GATCTGCGAG GAAGCCGCCT ACTCCAACCC CAGCCTGCCT 
CTGGTGCACC CCCCATCCCA TACCAAAGGG CCTGCACAGA CTCCAGCCGA GCCCACGCCT 
GGCTACGAGA TGGGCCAGCG GAAGCGCCTC ATCTCCTCTG TGGAGGACTT CACCGAGTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028697323.1
CDS	204..5909
Translation	MLRSGRGATCCSFSPPGRRGLLPTPPRSSPGSQAPVPRQHPGAQGVHAGLLRTTHPGGPRGPRHVTQGSPAFPLEGCLELQLPAGSVAALRAAPLPEVRVPLPAASGARGVRLSPRRGGPARGGAAREGCSGASWPTMAKPASKDSGLKEKFRILFGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLSNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIIQGQGERLGVLRALFFKVIKDYPSNEDLHERLDVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKGPAQTPAEPTPGYEMGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_028841490.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028841490.1

ORF Insert Sequence:

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAGGCTCCGT GGCGGCCCTC CGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
CCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAGCAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACCGCCGAA 
ATCCTGAGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTATATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCGTCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCGGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGCA GGATACAGAC GTCCCTCACC AGTGCCAGCC TGGGGTCTGC AGACGAGAAC 
TCCGTGGCCC AGGCTGACGA TAGCCTGAAA AGCCTCCATC TGGAGCTCAC GGAAACCTGT 
CTGGACATGA TGGCCCGATA CGTCTTCTCC AACTTCACAG CTGTCCCGAA GAGGTCTCCT 
GTGGGCGAGT TCCTCCTGGC GGGTGGCAGG ACCAAAACCT GGCTGGTTGG GAACAAGCTT 
GTCACTGTGA CGACGAGTGT GGGAACCGGG ACCCGGTCGT TACTAGGCCT GGACTCGGGG 
GAGCTGCAGT CCGGCCCGGA GTCGAGCTCC AGCCCCGGCA TGAATGTGAG ACAGACGAAG 
GAGGCGCCAG CCAAGCTGGA GTCCCAGGCT GGGCAGCAGG TGTCCCGTGG GGCCCGGGAC 
CGGGTCCGTT CTATGTCAGG GGGCCATGGT CTTCGAGTTG GCGCCCTGGA TGTGCCGGCC 
TCCCAGTTCC CGGGCAGTGC CGCTTCTCCA GGACCACAGA CTGCACCAGC CGCGAAGCCT 
GAGAAGGCCT CGGCCGGCGC CCGGCTTCCT GTGCAGGAGA AGACGAACCT GGCCGCCTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCGGAG ATCCTGGTCC GGAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCTTTCT CCTCGGACAT CAACAACATG 
CCCCTGCAGG AGCTGTCCAA CGCCCTCATG GCAGCTGAGC GCTTCAAGGA GCACCGGGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCA GCAGCCAGCA CGGCCAAACC CCCTCCTCTG 
CCTCGCTCCA ACACAGTGGC CTCTTTCTCC TCCCTGTACC AGTCCAGCTG CCAAGGACAG 
CTGCACAGGA GCGTTTCCTG GGCAGACTCC GCCGTGGTCA TGGAGGAGGG AAGTCCTGGC 
GAGGTTCCTG TGCCAGTGGA GCCCCCAGGA TTGGAGGACT TTGAGGCGGC GCTAGGCGTG 
GACAGGCGCA CAGACGCCTA CAGCAGGTCG TCCTCAACCT CCAGCCAGGA GGAGAAGTCA 
TTCCACGCAG AGGAGCTGGT TGCCGGGGGA ATCCCCATTG AACGAGCCGT CTCCTCGGAG 
GGTGGCCGGC CCTCTGTGGA CCTCTCCTTC CAGCCCTCAC AGCCCCTGAG CAAGTCTAGC 
TCCTCTCCTG AGCTGCAGAC CCTGCAGGAC ATCCTCGGGG ACCCTGGGGA CAAGGCTGAC 
GTGGGCCGGC TGAGCCCTGA GGTTAAGGCC CGTTCACAGT CAGGGATCCT GGACGGGGAA 
AGTGCTGCCT GGCCAGCCTC GGGCGAAGAC AGTCGGGGCC AGCCCGAGGG TCCCTTGCCT 
TCCAGCTCCC CCCGCTCACC CAGTGGCCTC CGGCCCCGCG GTTACACCAT CTCCGACTCG 
GCCCCATCAC GCAGGGGCAG GAGGGTAGAA AGGGATGCCT TTAAGAGCAG AGCCACAGCT 
TCCAATGCAG AGAAAGTGCC AGGCATCAAC CCCAGTTTCG TGTTCCTGCA GCTCTACCAT 
TCGCCTTTCT TTGGCGACGA GTCAAACAAG CCAATCCTGC TGCCCAATGA GTCACAGTCC 
TTTGAGCGGT CGGTGCAGCT CCTCGACCAG ATCCCATCAT ACGACACCCA CAAGATCGCC 
GTCCTGTATG TGGGAGAAGG CCAGAGCAAC AGTGAGCTCG CCATCCTGTC CAATGAGCAT 
GGCTCCTACA GGTACACGGA GTTCCTGACG GGCCTGGGCC GGCTCATCGA GCTCAAGGAC 
TGCCAGCCAG ACAAGGTGTA CCTGGGCGGC CTGGACGTGT GTGGTGAGGA CGGCCAGTTC 
ACCTACTGCT GGCACGACGA CATCATGCAA GCCGTCTTCC ACATCGCCAC CCTGATGCCC 
ACCAAGGACG TGGACAAGCA TCGTTGCGAC AAGAAGCGCC ACCTGGGCAA CGATTTCGTG 
TCCATTGTCT ACAATGACTC TGGCGAGGAC TTCAAGCTCG GCACCATCAA GGGCCAGTTC 
AACTTTGTCC ACGTGATCAT CACCCCGCTG GATTATGAGT GCAACCTGGT GTCCCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAGATCGT GTCTGACCGC 
AACCTGCCTT TTGTGGCCCG TCAGATGGCC CTGCATGCAA ACATGGCCTC ACAGGTGCAC 
CACAGCCGCT CCAACCCCAC CGACATCTAC CCCTCCAAGT GGATTGCCCG GCTCCGCCAC 
ATCAAGAGAC TCCGCCAGCG GATCTGCGAG GAAGCCGCCT ACTCCAACCC CAGCCTGCCT 
CTGGTGCACC CCCCATCCCA TACCAAAGGG CCTGCACAGA CTCCAGCCGA GCCCACGCCT 
GGCTACGAGA TGGGCCAGCG GAAGCGCCTC ATCTCCTCTG TGGAGGACTT CACCGAGTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_028841491.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028697324.1 Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X13, mRNA.

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CloneID	OMb81174
Clone ID Related Accession (Same CDS sequence)	XM_028841491.1
Accession Version	XM_028841491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAGGCTCCGT GGCGGCCCTC CGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
CCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAGCAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACCGCCGAA 
ATCCTGAGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTATATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCGTCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCGGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGGA TACAGACGTC CCTCACCAGT GCCAGCCTGG GGTCTGCAGA CGAGAACTCC 
GTGGCCCAGG CTGACGATAG CCTGAAAAGC CTCCATCTGG AGCTCACGGA AACCTGTCTG 
GACATGATGG CCCGATACGT CTTCTCCAAC TTCACAGCTG TCCCGAAGAG GTCTCCTGTG 
GGCGAGTTCC TCCTGGCGGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACTGTGACGA CGAGTGTGGG AACCGGGACC CGGTCGTTAC TAGGCCTGGA CTCGGGGGAG 
CTGCAGTCCG GCCCGGAGTC GAGCTCCAGC CCCGGCATGA ATGTGAGACA GACGAAGGAG 
GCGCCAGCCA AGCTGGAGTC CCAGGCTGGG CAGCAGGTGT CCCGTGGGGC CCGGGACCGG 
GTCCGTTCTA TGTCAGGGGG CCATGGTCTT CGAGTTGGCG CCCTGGATGT GCCGGCCTCC 
CAGTTCCCGG GCAGTGCCGC TTCTCCAGGA CCACAGACTG CACCAGCCGC GAAGCCTGAG 
AAGGCCTCGG CCGGCGCCCG GCTTCCTGTG CAGGAGAAGA CGAACCTGGC CGCCTACGTG 
CCCCTGCTGA CCCAGGGCTG GGCGGAGATC CTGGTCCGGA GGCCCACAGG GAACACCAGC 
TGGCTGATGA GCCTGGAGAA CCCGCTCAGC CCTTTCTCCT CGGACATCAA CAACATGCCC 
CTGCAGGAGC TGTCCAACGC CCTCATGGCA GCTGAGCGCT TCAAGGAGCA CCGGGACACA 
GCCCTGTACA AGTCGCTGTC GGTGCCAGCA GCCAGCACGG CCAAACCCCC TCCTCTGCCT 
CGCTCCAACA CAGTGGCCTC TTTCTCCTCC CTGTACCAGT CCAGCTGCCA AGGACAGCTG 
CACAGGAGCG TTTCCTGGGC AGACTCCGCC GTGGTCATGG AGGAGGGAAG TCCTGGCGAG 
GTTCCTGTGC CAGTGGAGCC CCCAGGATTG GAGGACTTTG AGGCGGCGCT AGGCGTGGAC 
AGGCGCACAG ACGCCTACAG CAGGTCGTCC TCAACCTCCA GCCAGGAGGA GAAGTCATTC 
CACGCAGAGG AGCTGGTTGC CGGGGGAATC CCCATTGAAC GAGCCGTCTC CTCGGAGGGT 
GGCCGGCCCT CTGTGGACCT CTCCTTCCAG CCCTCACAGC CCCTGAGCAA GTCTAGCTCC 
TCTCCTGAGC TGCAGACCCT GCAGGACATC CTCGGGGACC CTGGGGACAA GGCTGACGTG 
GGCCGGCTGA GCCCTGAGGT TAAGGCCCGT TCACAGTCAG GGATCCTGGA CGGGGAAAGT 
GCTGCCTGGC CAGCCTCGGG CGAAGACAGT CGGGGCCAGC CCGAGGGTCC CTTGCCTTCC 
AGCTCCCCCC GCTCACCCAG TGGCCTCCGG CCCCGCGGTT ACACCATCTC CGACTCGGCC 
CCATCACGCA GGGGCAGGAG GGTAGAAAGG GATGCCTTTA AGAGCAGAGC CACAGCTTCC 
AATGCAGAGA AAGTGCCAGG CATCAACCCC AGTTTCGTGT TCCTGCAGCT CTACCATTCG 
CCTTTCTTTG GCGACGAGTC AAACAAGCCA ATCCTGCTGC CCAATGAGTC ACAGTCCTTT 
GAGCGGTCGG TGCAGCTCCT CGACCAGATC CCATCATACG ACACCCACAA GATCGCCGTC 
CTGTATGTGG GAGAAGGCCA GAGCAACAGT GAGCTCGCCA TCCTGTCCAA TGAGCATGGC 
TCCTACAGGT ACACGGAGTT CCTGACGGGC CTGGGCCGGC TCATCGAGCT CAAGGACTGC 
CAGCCAGACA AGGTGTACCT GGGCGGCCTG GACGTGTGTG GTGAGGACGG CCAGTTCACC 
TACTGCTGGC ACGACGACAT CATGCAAGCC GTCTTCCACA TCGCCACCCT GATGCCCACC 
AAGGACGTGG ACAAGCATCG TTGCGACAAG AAGCGCCACC TGGGCAACGA TTTCGTGTCC 
ATTGTCTACA ATGACTCTGG CGAGGACTTC AAGCTCGGCA CCATCAAGGG CCAGTTCAAC 
TTTGTCCACG TGATCATCAC CCCGCTGGAT TATGAGTGCA ACCTGGTGTC CCTGCAGTGC 
AGGAAAGACA TGGAGGGCCT TGTGGACACC AGCGTGGCCA AGATCGTGTC TGACCGCAAC 
CTGCCTTTTG TGGCCCGTCA GATGGCCCTG CATGCAAACA TGGCCTCACA GGTGCACCAC 
AGCCGCTCCA ACCCCACCGA CATCTACCCC TCCAAGTGGA TTGCCCGGCT CCGCCACATC 
AAGAGACTCC GCCAGCGGAT CTGCGAGGAA GCCGCCTACT CCAACCCCAG CCTGCCTCTG 
GTGCACCCCC CATCCCATAC CAAAGGGCCT GCACAGACTC CAGCCGAGCC CACGCCTGGC 
TACGAGATGG GCCAGCGGAA GCGCCTCATC TCCTCTGTGG AGGACTTCAC CGAGTTCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028697324.1
CDS	204..5906
Translation	MLRSGRGATCCSFSPPGRRGLLPTPPRSSPGSQAPVPRQHPGAQGVHAGLLRTTHPGGPRGPRHVTQGSPAFPLEGCLELQLPAGSVAALRAAPLPEVRVPLPAASGARGVRLSPRRGGPARGGAAREGCSGASWPTMAKPASKDSGLKEKFRILFGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLSNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIIQGQGERLGVLRALFFKVIKDYPSNEDLHERLDVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKGPAQTPAEPTPGYEMGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_028841491.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028841491.1

ORF Insert Sequence:

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAGGCTCCGT GGCGGCCCTC CGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
CCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAGCAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACCGCCGAA 
ATCCTGAGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTATATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCGTCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCGGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGGA TACAGACGTC CCTCACCAGT GCCAGCCTGG GGTCTGCAGA CGAGAACTCC 
GTGGCCCAGG CTGACGATAG CCTGAAAAGC CTCCATCTGG AGCTCACGGA AACCTGTCTG 
GACATGATGG CCCGATACGT CTTCTCCAAC TTCACAGCTG TCCCGAAGAG GTCTCCTGTG 
GGCGAGTTCC TCCTGGCGGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACTGTGACGA CGAGTGTGGG AACCGGGACC CGGTCGTTAC TAGGCCTGGA CTCGGGGGAG 
CTGCAGTCCG GCCCGGAGTC GAGCTCCAGC CCCGGCATGA ATGTGAGACA GACGAAGGAG 
GCGCCAGCCA AGCTGGAGTC CCAGGCTGGG CAGCAGGTGT CCCGTGGGGC CCGGGACCGG 
GTCCGTTCTA TGTCAGGGGG CCATGGTCTT CGAGTTGGCG CCCTGGATGT GCCGGCCTCC 
CAGTTCCCGG GCAGTGCCGC TTCTCCAGGA CCACAGACTG CACCAGCCGC GAAGCCTGAG 
AAGGCCTCGG CCGGCGCCCG GCTTCCTGTG CAGGAGAAGA CGAACCTGGC CGCCTACGTG 
CCCCTGCTGA CCCAGGGCTG GGCGGAGATC CTGGTCCGGA GGCCCACAGG GAACACCAGC 
TGGCTGATGA GCCTGGAGAA CCCGCTCAGC CCTTTCTCCT CGGACATCAA CAACATGCCC 
CTGCAGGAGC TGTCCAACGC CCTCATGGCA GCTGAGCGCT TCAAGGAGCA CCGGGACACA 
GCCCTGTACA AGTCGCTGTC GGTGCCAGCA GCCAGCACGG CCAAACCCCC TCCTCTGCCT 
CGCTCCAACA CAGTGGCCTC TTTCTCCTCC CTGTACCAGT CCAGCTGCCA AGGACAGCTG 
CACAGGAGCG TTTCCTGGGC AGACTCCGCC GTGGTCATGG AGGAGGGAAG TCCTGGCGAG 
GTTCCTGTGC CAGTGGAGCC CCCAGGATTG GAGGACTTTG AGGCGGCGCT AGGCGTGGAC 
AGGCGCACAG ACGCCTACAG CAGGTCGTCC TCAACCTCCA GCCAGGAGGA GAAGTCATTC 
CACGCAGAGG AGCTGGTTGC CGGGGGAATC CCCATTGAAC GAGCCGTCTC CTCGGAGGGT 
GGCCGGCCCT CTGTGGACCT CTCCTTCCAG CCCTCACAGC CCCTGAGCAA GTCTAGCTCC 
TCTCCTGAGC TGCAGACCCT GCAGGACATC CTCGGGGACC CTGGGGACAA GGCTGACGTG 
GGCCGGCTGA GCCCTGAGGT TAAGGCCCGT TCACAGTCAG GGATCCTGGA CGGGGAAAGT 
GCTGCCTGGC CAGCCTCGGG CGAAGACAGT CGGGGCCAGC CCGAGGGTCC CTTGCCTTCC 
AGCTCCCCCC GCTCACCCAG TGGCCTCCGG CCCCGCGGTT ACACCATCTC CGACTCGGCC 
CCATCACGCA GGGGCAGGAG GGTAGAAAGG GATGCCTTTA AGAGCAGAGC CACAGCTTCC 
AATGCAGAGA AAGTGCCAGG CATCAACCCC AGTTTCGTGT TCCTGCAGCT CTACCATTCG 
CCTTTCTTTG GCGACGAGTC AAACAAGCCA ATCCTGCTGC CCAATGAGTC ACAGTCCTTT 
GAGCGGTCGG TGCAGCTCCT CGACCAGATC CCATCATACG ACACCCACAA GATCGCCGTC 
CTGTATGTGG GAGAAGGCCA GAGCAACAGT GAGCTCGCCA TCCTGTCCAA TGAGCATGGC 
TCCTACAGGT ACACGGAGTT CCTGACGGGC CTGGGCCGGC TCATCGAGCT CAAGGACTGC 
CAGCCAGACA AGGTGTACCT GGGCGGCCTG GACGTGTGTG GTGAGGACGG CCAGTTCACC 
TACTGCTGGC ACGACGACAT CATGCAAGCC GTCTTCCACA TCGCCACCCT GATGCCCACC 
AAGGACGTGG ACAAGCATCG TTGCGACAAG AAGCGCCACC TGGGCAACGA TTTCGTGTCC 
ATTGTCTACA ATGACTCTGG CGAGGACTTC AAGCTCGGCA CCATCAAGGG CCAGTTCAAC 
TTTGTCCACG TGATCATCAC CCCGCTGGAT TATGAGTGCA ACCTGGTGTC CCTGCAGTGC 
AGGAAAGACA TGGAGGGCCT TGTGGACACC AGCGTGGCCA AGATCGTGTC TGACCGCAAC 
CTGCCTTTTG TGGCCCGTCA GATGGCCCTG CATGCAAACA TGGCCTCACA GGTGCACCAC 
AGCCGCTCCA ACCCCACCGA CATCTACCCC TCCAAGTGGA TTGCCCGGCT CCGCCACATC 
AAGAGACTCC GCCAGCGGAT CTGCGAGGAA GCCGCCTACT CCAACCCCAG CCTGCCTCTG 
GTGCACCCCC CATCCCATAC CAAAGGGCCT GCACAGACTC CAGCCGAGCC CACGCCTGGC 
TACGAGATGG GCCAGCGGAA GCGCCTCATC TCCTCTGTGG AGGACTTCAC CGAGTTCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_028841492.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028697325.1 Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X14, mRNA.

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CloneID	OMb81175
Clone ID Related Accession (Same CDS sequence)	XM_028841492.1
Accession Version	XM_028841492.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAGGAT TCTGTTTGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGCA AACAGACGGA ATTTATCATC 
ACCGCCGAAA TCCTGAGAGA GCTGAGTGTG GAATGCGGCC TCAGCAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTTGCGGA TCTGTTGCAG CCAGAACGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCATTCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGG 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTCCA CGAGAGGCTG 
GACGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCGGACTTTG TCCTGCAGTG GATGGATGTC GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA CAGCTGTTAC CTCGATGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGCC TGCTCTGCGT CCGGACCGCA TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTATATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCGGTGCTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGAC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GCTGACCACG GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCAGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTACAGA GCGCAGTCCA TTCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAC 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCTCAGCTCT CCCACATCCC CGAGGATAAA 
GACCATCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGTCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGTTGGAAGA AAGGGATGTG GCCGCGTACT CGGCGTCCTT GGAGGATGTG 
AAGACAGCTG TCCTGGGGCT TCTGGTCATC CTTCAGACTA AGCTGTACAC CCTGCCTGCG 
AGCCATGCCA CGCGCGTTTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC CAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTCGGC CTGCCCAACA AGGATGGGGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCACCT GGCCCGGCGC CTGCAGGCCC GGCTGTGCGG 
CTGGGCTCCC TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTACAAA 
GTACTCATCT TTACCTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CGGAAGGCTT CTCCAGAACT 
GACCTGCACC TGGCTGTGGT TCCAGTGCTG ACGGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTAGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATGATC 
AAGGCGCTGC CCGTCCTGGT GGTGAAGCTC ACACACATCT CGGCCACAGC CAGCATGGCC 
GTCCCGCTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCAGCTCTA CAGGAACTTC 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCACTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT TATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGGC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATACAGACGT CCCTCACCAG TGCCAGCCTG 
GGGTCTGCAG ACGAGAACTC CGTGGCCCAG GCTGACGATA GCCTGAAAAG CCTCCATCTG 
GAGCTCACGG AAACCTGTCT GGACATGATG GCCCGATACG TCTTCTCCAA CTTCACAGCT 
GTCCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTGGCGG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACGAGTGTGG GAACCGGGAC CCGGTCGTTA 
CTAGGCCTGG ACTCGGGGGA GCTGCAGTCC GGCCCGGAGT CGAGCTCCAG CCCCGGCATG 
AATGTGAGAC AGACGAAGGA GGCGCCAGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGACCG GGTCCGTTCT ATGTCAGGGG GCCATGGTCT TCGAGTTGGC 
GCCCTGGATG TGCCGGCCTC CCAGTTCCCG GGCAGTGCCG CTTCTCCAGG ACCACAGACT 
GCACCAGCCG CGAAGCCTGA GAAGGCCTCG GCCGGCGCCC GGCTTCCTGT GCAGGAGAAG 
ACGAACCTGG CCGCCTACGT GCCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCCAACG CCCTCATGGC AGCTGAGCGC 
TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCGCTGT CGGTGCCAGC AGCCAGCACG 
GCCAAACCCC CTCCTCTGCC TCGCTCCAAC ACAGTGGCCT CTTTCTCCTC CCTGTACCAG 
TCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCCGC CGTGGTCATG 
GAGGAGGGAA GTCCTGGCGA GGTTCCTGTG CCAGTGGAGC CCCCAGGATT GGAGGACTTT 
GAGGCGGCGC TAGGCGTGGA CAGGCGCACA GACGCCTACA GCAGGTCGTC CTCAACCTCC 
AGCCAGGAGG AGAAGTCATT CCACGCAGAG GAGCTGGTTG CCGGGGGAAT CCCCATTGAA 
CGAGCCGTCT CCTCGGAGGG TGGCCGGCCC TCTGTGGACC TCTCCTTCCA GCCCTCACAG 
CCCCTGAGCA AGTCTAGCTC CTCTCCTGAG CTGCAGACCC TGCAGGACAT CCTCGGGGAC 
CCTGGGGACA AGGCTGACGT GGGCCGGCTG AGCCCTGAGG TTAAGGCCCG TTCACAGTCA 
GGGATCCTGG ACGGGGAAAG TGCTGCCTGG CCAGCCTCGG GCGAAGACAG TCGGGGCCAG 
CCCGAGGGTC CCTTGCCTTC CAGCTCCCCC CGCTCACCCA GTGGCCTCCG GCCCCGCGGT 
TACACCATCT CCGACTCGGC CCCATCACGC AGGGGCAGGA GGGTAGAAAG GGATGCCTTT 
AAGAGCAGAG CCACAGCTTC CAATGCAGAG AAAGTGCCAG GCATCAACCC CAGGTGGGCC 
TCTTGCTTCC GGGCGGGGCT CCTGATACCA TCCTGCAGGA ACCTGGTGCC TCAGTTGCCC 
CAGGCCGAGC AGGCTGGGGA GGGTCCTCGC CTGTGCCCTA GGGCTGGCTG GAGCCCCTGG 
CAAGGCGGTG GAGTGGGAGA TGGCCAGGTT CTGTGTTCCT CCCTGGGCTG TGGCTGCCCT 
GGCCAGGCCT TCACCTGGGT GCCCACTGTC TCCTCCCTGT GCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CGCCTTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT GGGAGAAGGC CAGAGCAACA GTGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTCAAGGACT GCCAGCCAGA CAAGGTGTAC CTGGGCGGCC TGGACGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGACGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAT CGTTGCGACA AGAAGCGCCA CCTGGGCAAC 
GATTTCGTGT CCATTGTCTA CAATGACTCT GGCGAGGACT TCAAGCTCGG CACCATCAAG 
GGCCAGTTCA ACTTTGTCCA CGTGATCATC ACCCCGCTGG ATTATGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACCTGCCTTT TGTGGCCCGT CAGATGGCCC TGCATGCAAA CATGGCCTCA 
CAGGTGCACC ACAGCCGCTC CAACCCCACC GACATCTACC CCTCCAAGTG GATTGCCCGG 
CTCCGCCACA TCAAGAGACT CCGCCAGCGG ATCTGCGAGG AAGCCGCCTA CTCCAACCCC 
AGCCTGCCTC TGGTGCACCC CCCATCCCAT ACCAAAGGGC CTGCACAGAC TCCAGCCGAG 
CCCACGCCTG GCTACGAGAT GGGCCAGCGG AAGCGCCTCA TCTCCTCTGT GGAGGACTTC 
ACCGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028697325.1
CDS	429..6083
Translation	MAKPASKDSGLKEKFRILFGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLSNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIIQGQGERLGVLRALFFKVIKDYPSNEDLHERLDVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASNAEKVPGINPRWASCFRAGLLIPSCRNLVPQLPQAEQAGEGPRLCPRAGWSPWQGGGVGDGQVLCSSLGCGCPGQAFTWVPTVSSLCSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKGPAQTPAEPTPGYEMGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_028841492.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028841492.1

ORF Insert Sequence:

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAGGAT TCTGTTTGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGCA AACAGACGGA ATTTATCATC 
ACCGCCGAAA TCCTGAGAGA GCTGAGTGTG GAATGCGGCC TCAGCAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTTGCGGA TCTGTTGCAG CCAGAACGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCATTCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGG 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTCCA CGAGAGGCTG 
GACGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCGGACTTTG TCCTGCAGTG GATGGATGTC GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA CAGCTGTTAC CTCGATGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGCC TGCTCTGCGT CCGGACCGCA TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTATATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCGGTGCTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGAC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GCTGACCACG GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCAGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTACAGA GCGCAGTCCA TTCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAC 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCTCAGCTCT CCCACATCCC CGAGGATAAA 
GACCATCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGTCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGTTGGAAGA AAGGGATGTG GCCGCGTACT CGGCGTCCTT GGAGGATGTG 
AAGACAGCTG TCCTGGGGCT TCTGGTCATC CTTCAGACTA AGCTGTACAC CCTGCCTGCG 
AGCCATGCCA CGCGCGTTTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC CAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTCGGC CTGCCCAACA AGGATGGGGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCACCT GGCCCGGCGC CTGCAGGCCC GGCTGTGCGG 
CTGGGCTCCC TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTACAAA 
GTACTCATCT TTACCTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CGGAAGGCTT CTCCAGAACT 
GACCTGCACC TGGCTGTGGT TCCAGTGCTG ACGGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTAGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATGATC 
AAGGCGCTGC CCGTCCTGGT GGTGAAGCTC ACACACATCT CGGCCACAGC CAGCATGGCC 
GTCCCGCTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCAGCTCTA CAGGAACTTC 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCACTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT TATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGGC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATACAGACGT CCCTCACCAG TGCCAGCCTG 
GGGTCTGCAG ACGAGAACTC CGTGGCCCAG GCTGACGATA GCCTGAAAAG CCTCCATCTG 
GAGCTCACGG AAACCTGTCT GGACATGATG GCCCGATACG TCTTCTCCAA CTTCACAGCT 
GTCCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTGGCGG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACGAGTGTGG GAACCGGGAC CCGGTCGTTA 
CTAGGCCTGG ACTCGGGGGA GCTGCAGTCC GGCCCGGAGT CGAGCTCCAG CCCCGGCATG 
AATGTGAGAC AGACGAAGGA GGCGCCAGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGACCG GGTCCGTTCT ATGTCAGGGG GCCATGGTCT TCGAGTTGGC 
GCCCTGGATG TGCCGGCCTC CCAGTTCCCG GGCAGTGCCG CTTCTCCAGG ACCACAGACT 
GCACCAGCCG CGAAGCCTGA GAAGGCCTCG GCCGGCGCCC GGCTTCCTGT GCAGGAGAAG 
ACGAACCTGG CCGCCTACGT GCCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCCAACG CCCTCATGGC AGCTGAGCGC 
TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCGCTGT CGGTGCCAGC AGCCAGCACG 
GCCAAACCCC CTCCTCTGCC TCGCTCCAAC ACAGTGGCCT CTTTCTCCTC CCTGTACCAG 
TCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCCGC CGTGGTCATG 
GAGGAGGGAA GTCCTGGCGA GGTTCCTGTG CCAGTGGAGC CCCCAGGATT GGAGGACTTT 
GAGGCGGCGC TAGGCGTGGA CAGGCGCACA GACGCCTACA GCAGGTCGTC CTCAACCTCC 
AGCCAGGAGG AGAAGTCATT CCACGCAGAG GAGCTGGTTG CCGGGGGAAT CCCCATTGAA 
CGAGCCGTCT CCTCGGAGGG TGGCCGGCCC TCTGTGGACC TCTCCTTCCA GCCCTCACAG 
CCCCTGAGCA AGTCTAGCTC CTCTCCTGAG CTGCAGACCC TGCAGGACAT CCTCGGGGAC 
CCTGGGGACA AGGCTGACGT GGGCCGGCTG AGCCCTGAGG TTAAGGCCCG TTCACAGTCA 
GGGATCCTGG ACGGGGAAAG TGCTGCCTGG CCAGCCTCGG GCGAAGACAG TCGGGGCCAG 
CCCGAGGGTC CCTTGCCTTC CAGCTCCCCC CGCTCACCCA GTGGCCTCCG GCCCCGCGGT 
TACACCATCT CCGACTCGGC CCCATCACGC AGGGGCAGGA GGGTAGAAAG GGATGCCTTT 
AAGAGCAGAG CCACAGCTTC CAATGCAGAG AAAGTGCCAG GCATCAACCC CAGGTGGGCC 
TCTTGCTTCC GGGCGGGGCT CCTGATACCA TCCTGCAGGA ACCTGGTGCC TCAGTTGCCC 
CAGGCCGAGC AGGCTGGGGA GGGTCCTCGC CTGTGCCCTA GGGCTGGCTG GAGCCCCTGG 
CAAGGCGGTG GAGTGGGAGA TGGCCAGGTT CTGTGTTCCT CCCTGGGCTG TGGCTGCCCT 
GGCCAGGCCT TCACCTGGGT GCCCACTGTC TCCTCCCTGT GCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CGCCTTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT GGGAGAAGGC CAGAGCAACA GTGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTCAAGGACT GCCAGCCAGA CAAGGTGTAC CTGGGCGGCC TGGACGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGACGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAT CGTTGCGACA AGAAGCGCCA CCTGGGCAAC 
GATTTCGTGT CCATTGTCTA CAATGACTCT GGCGAGGACT TCAAGCTCGG CACCATCAAG 
GGCCAGTTCA ACTTTGTCCA CGTGATCATC ACCCCGCTGG ATTATGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACCTGCCTTT TGTGGCCCGT CAGATGGCCC TGCATGCAAA CATGGCCTCA 
CAGGTGCACC ACAGCCGCTC CAACCCCACC GACATCTACC CCTCCAAGTG GATTGCCCGG 
CTCCGCCACA TCAAGAGACT CCGCCAGCGG ATCTGCGAGG AAGCCGCCTA CTCCAACCCC 
AGCCTGCCTC TGGTGCACCC CCCATCCCAT ACCAAAGGGC CTGCACAGAC TCCAGCCGAG 
CCCACGCCTG GCTACGAGAT GGGCCAGCGG AAGCGCCTCA TCTCCTCTGT GGAGGACTTC 
ACCGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015125356.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014980842.2 Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OMb81176
Clone ID Related Accession (Same CDS sequence)	XM_015125356.2
Accession Version	XM_015125356.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_015125356.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAGGCTCCGT GGCGGCCCTC CGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
CCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAGCAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACCGCCGAA 
ATCCTGAGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTATATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCGTCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCGGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGCA GGATACAGAC GTCCCTCACC AGTGCCAGCC TGGGGTCTGC AGACGAGAAC 
TCCGTGGCCC AGGCTGACGA TAGCCTGAAA AGCCTCCATC TGGAGCTCAC GGAAACCTGT 
CTGGACATGA TGGCCCGATA CGTCTTCTCC AACTTCACAG CTGTCCCGAA GAGGTCTCCT 
GTGGGCGAGT TCCTCCTGGC GGGTGGCAGG ACCAAAACCT GGCTGGTTGG GAACAAGCTT 
GTCACTGTGA CGACGAGTGT GGGAACCGGG ACCCGGTCGT TACTAGGCCT GGACTCGGGG 
GAGCTGCAGT CCGGCCCGGA GTCGAGCTCC AGCCCCGGCA TGAATGTGAG ACAGACGAAG 
GAGGCGCCAG CCAAGCTGGA GTCCCAGGCT GGGCAGCAGG TGTCCCGTGG GGCCCGGGAC 
CGGGTCCGTT CTATGTCAGG GGGCCATGGT CTTCGAGTTG GCGCCCTGGA TGTGCCGGCC 
TCCCAGTTCC CGGGCAGTGC CGCTTCTCCA GGACCACAGA CTGCACCAGC CGCGAAGCCT 
GAGAAGGCCT CGGCCGGCGC CCGGCTTCCT GTGCAGGAGA AGACGAACCT GGCCGCCTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCGGAG ATCCTGGTCC GGAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCTTTCT CCTCGGACAT CAACAACATG 
CCCCTGCAGG AGCTGTCCAA CGCCCTCATG GCAGCTGAGC GCTTCAAGGA GCACCGGGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCA GCAGCCAGCA CGGCCAAACC CCCTCCTCTG 
CCTCGCTCCA ACACAGACTC CGCCGTGGTC ATGGAGGAGG GAAGTCCTGG CGAGGTTCCT 
GTGCCAGTGG AGCCCCCAGG ATTGGAGGAC TTTGAGGCGG CGCTAGGCGT GGACAGGCGC 
ACAGACGCCT ACAGCAGGTC GTCCTCAACC TCCAGCCAGG AGGAGAAGTC ATTCCACGCA 
GAGGAGCTGG TTGCCGGGGG AATCCCCATT GAACGAGCCG TCTCCTCGGA GGGTGGCCGG 
CCCTCTGTGG ACCTCTCCTT CCAGCCCTCA CAGCCCCTGA GCAAGTCTAG CTCCTCTCCT 
GAGCTGCAGA CCCTGCAGGA CATCCTCGGG GACCCTGGGG ACAAGGCTGA CGTGGGCCGG 
CTGAGCCCTG AGGTTAAGGC CCGTTCACAG TCAGGGATCC TGGACGGGGA AAGTGCTGCC 
TGGCCAGCCT CGGGCGAAGA CAGTCGGGGC CAGCCCGAGG GTCCCTTGCC TTCCAGCTCC 
CCCCGCTCAC CCAGTGGCCT CCGGCCCCGC GGTTACACCA TCTCCGACTC GGCCCCATCA 
CGCAGGGGCA GGAGGGTAGA AAGGGATGCC TTTAAGAGCA GAGCCACAGC TTCCAATGCA 
GAGAAAGTGC CAGGCATCAA CCCCAGTTTC GTGTTCCTGC AGCTCTACCA TTCGCCTTTC 
TTTGGCGACG AGTCAAACAA GCCAATCCTG CTGCCCAATG AGTCACAGTC CTTTGAGCGG 
TCGGTGCAGC TCCTCGACCA GATCCCATCA TACGACACCC ACAAGATCGC CGTCCTGTAT 
GTGGGAGAAG GCCAGAGCAA CAGTGAGCTC GCCATCCTGT CCAATGAGCA TGGCTCCTAC 
AGGTACACGG AGTTCCTGAC GGGCCTGGGC CGGCTCATCG AGCTCAAGGA CTGCCAGCCA 
GACAAGGTGT ACCTGGGCGG CCTGGACGTG TGTGGTGAGG ACGGCCAGTT CACCTACTGC 
TGGCACGACG ACATCATGCA AGCCGTCTTC CACATCGCCA CCCTGATGCC CACCAAGGAC 
GTGGACAAGC ATCGTTGCGA CAAGAAGCGC CACCTGGGCA ACGATTTCGT GTCCATTGTC 
TACAATGACT CTGGCGAGGA CTTCAAGCTC GGCACCATCA AGGGCCAGTT CAACTTTGTC 
CACGTGATCA TCACCCCGCT GGATTATGAG TGCAACCTGG TGTCCCTGCA GTGCAGGAAA 
GACATGGAGG GCCTTGTGGA CACCAGCGTG GCCAAGATCG TGTCTGACCG CAACCTGCCT 
TTTGTGGCCC GTCAGATGGC CCTGCATGCA AACATGGCCT CACAGGTGCA CCACAGCCGC 
TCCAACCCCA CCGACATCTA CCCCTCCAAG TGGATTGCCC GGCTCCGCCA CATCAAGAGA 
CTCCGCCAGC GGATCTGCGA GGAAGCCGCC TACTCCAACC CCAGCCTGCC TCTGGTGCAC 
CCCCCATCCC ATACCAAAGG GCCTGCACAG ACTCCAGCCG AGCCCACGCC TGGCTACGAG 
ATGGGCCAGC GGAAGCGCCT CATCTCCTCT GTGGAGGACT TCACCGAGTT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014980842.2
CDS	204..5840
Translation	MLRSGRGATCCSFSPPGRRGLLPTPPRSSPGSQAPVPRQHPGAQGVHAGLLRTTHPGGPRGPRHVTQGSPAFPLEGCLELQLPAGSVAALRAAPLPEVRVPLPAASGARGVRLSPRRGGPARGGAAREGCSGASWPTMAKPASKDSGLKEKFRILFGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLSNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIIQGQGERLGVLRALFFKVIKDYPSNEDLHERLDVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTDSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKGPAQTPAEPTPGYEMGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_015125356.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015125356.2

ORF Insert Sequence:

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAGGCTCCGT GGCGGCCCTC CGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
CCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAGCAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACCGCCGAA 
ATCCTGAGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTATATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCGTCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCGGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGCA GGATACAGAC GTCCCTCACC AGTGCCAGCC TGGGGTCTGC AGACGAGAAC 
TCCGTGGCCC AGGCTGACGA TAGCCTGAAA AGCCTCCATC TGGAGCTCAC GGAAACCTGT 
CTGGACATGA TGGCCCGATA CGTCTTCTCC AACTTCACAG CTGTCCCGAA GAGGTCTCCT 
GTGGGCGAGT TCCTCCTGGC GGGTGGCAGG ACCAAAACCT GGCTGGTTGG GAACAAGCTT 
GTCACTGTGA CGACGAGTGT GGGAACCGGG ACCCGGTCGT TACTAGGCCT GGACTCGGGG 
GAGCTGCAGT CCGGCCCGGA GTCGAGCTCC AGCCCCGGCA TGAATGTGAG ACAGACGAAG 
GAGGCGCCAG CCAAGCTGGA GTCCCAGGCT GGGCAGCAGG TGTCCCGTGG GGCCCGGGAC 
CGGGTCCGTT CTATGTCAGG GGGCCATGGT CTTCGAGTTG GCGCCCTGGA TGTGCCGGCC 
TCCCAGTTCC CGGGCAGTGC CGCTTCTCCA GGACCACAGA CTGCACCAGC CGCGAAGCCT 
GAGAAGGCCT CGGCCGGCGC CCGGCTTCCT GTGCAGGAGA AGACGAACCT GGCCGCCTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCGGAG ATCCTGGTCC GGAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCTTTCT CCTCGGACAT CAACAACATG 
CCCCTGCAGG AGCTGTCCAA CGCCCTCATG GCAGCTGAGC GCTTCAAGGA GCACCGGGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCA GCAGCCAGCA CGGCCAAACC CCCTCCTCTG 
CCTCGCTCCA ACACAGACTC CGCCGTGGTC ATGGAGGAGG GAAGTCCTGG CGAGGTTCCT 
GTGCCAGTGG AGCCCCCAGG ATTGGAGGAC TTTGAGGCGG CGCTAGGCGT GGACAGGCGC 
ACAGACGCCT ACAGCAGGTC GTCCTCAACC TCCAGCCAGG AGGAGAAGTC ATTCCACGCA 
GAGGAGCTGG TTGCCGGGGG AATCCCCATT GAACGAGCCG TCTCCTCGGA GGGTGGCCGG 
CCCTCTGTGG ACCTCTCCTT CCAGCCCTCA CAGCCCCTGA GCAAGTCTAG CTCCTCTCCT 
GAGCTGCAGA CCCTGCAGGA CATCCTCGGG GACCCTGGGG ACAAGGCTGA CGTGGGCCGG 
CTGAGCCCTG AGGTTAAGGC CCGTTCACAG TCAGGGATCC TGGACGGGGA AAGTGCTGCC 
TGGCCAGCCT CGGGCGAAGA CAGTCGGGGC CAGCCCGAGG GTCCCTTGCC TTCCAGCTCC 
CCCCGCTCAC CCAGTGGCCT CCGGCCCCGC GGTTACACCA TCTCCGACTC GGCCCCATCA 
CGCAGGGGCA GGAGGGTAGA AAGGGATGCC TTTAAGAGCA GAGCCACAGC TTCCAATGCA 
GAGAAAGTGC CAGGCATCAA CCCCAGTTTC GTGTTCCTGC AGCTCTACCA TTCGCCTTTC 
TTTGGCGACG AGTCAAACAA GCCAATCCTG CTGCCCAATG AGTCACAGTC CTTTGAGCGG 
TCGGTGCAGC TCCTCGACCA GATCCCATCA TACGACACCC ACAAGATCGC CGTCCTGTAT 
GTGGGAGAAG GCCAGAGCAA CAGTGAGCTC GCCATCCTGT CCAATGAGCA TGGCTCCTAC 
AGGTACACGG AGTTCCTGAC GGGCCTGGGC CGGCTCATCG AGCTCAAGGA CTGCCAGCCA 
GACAAGGTGT ACCTGGGCGG CCTGGACGTG TGTGGTGAGG ACGGCCAGTT CACCTACTGC 
TGGCACGACG ACATCATGCA AGCCGTCTTC CACATCGCCA CCCTGATGCC CACCAAGGAC 
GTGGACAAGC ATCGTTGCGA CAAGAAGCGC CACCTGGGCA ACGATTTCGT GTCCATTGTC 
TACAATGACT CTGGCGAGGA CTTCAAGCTC GGCACCATCA AGGGCCAGTT CAACTTTGTC 
CACGTGATCA TCACCCCGCT GGATTATGAG TGCAACCTGG TGTCCCTGCA GTGCAGGAAA 
GACATGGAGG GCCTTGTGGA CACCAGCGTG GCCAAGATCG TGTCTGACCG CAACCTGCCT 
TTTGTGGCCC GTCAGATGGC CCTGCATGCA AACATGGCCT CACAGGTGCA CCACAGCCGC 
TCCAACCCCA CCGACATCTA CCCCTCCAAG TGGATTGCCC GGCTCCGCCA CATCAAGAGA 
CTCCGCCAGC GGATCTGCGA GGAAGCCGCC TACTCCAACC CCAGCCTGCC TCTGGTGCAC 
CCCCCATCCC ATACCAAAGG GCCTGCACAG ACTCCAGCCG AGCCCACGCC TGGCTACGAG 
ATGGGCCAGC GGAAGCGCCT CATCTCCTCT GTGGAGGACT TCACCGAGTT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015125357.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014980843.2 Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X16, mRNA.

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CloneID	OMb81177
Clone ID Related Accession (Same CDS sequence)	XM_015125357.2
Accession Version	XM_015125357.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_015125357.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAGGCTCCGT GGCGGCCCTC CGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
CCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAGCAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACCGCCGAA 
ATCCTGAGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTATATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCGTCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCGGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGGA TACAGACGTC CCTCACCAGT GCCAGCCTGG GGTCTGCAGA CGAGAACTCC 
GTGGCCCAGG CTGACGATAG CCTGAAAAGC CTCCATCTGG AGCTCACGGA AACCTGTCTG 
GACATGATGG CCCGATACGT CTTCTCCAAC TTCACAGCTG TCCCGAAGAG GTCTCCTGTG 
GGCGAGTTCC TCCTGGCGGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACTGTGACGA CGAGTGTGGG AACCGGGACC CGGTCGTTAC TAGGCCTGGA CTCGGGGGAG 
CTGCAGTCCG GCCCGGAGTC GAGCTCCAGC CCCGGCATGA ATGTGAGACA GACGAAGGAG 
GCGCCAGCCA AGCTGGAGTC CCAGGCTGGG CAGCAGGTGT CCCGTGGGGC CCGGGACCGG 
GTCCGTTCTA TGTCAGGGGG CCATGGTCTT CGAGTTGGCG CCCTGGATGT GCCGGCCTCC 
CAGTTCCCGG GCAGTGCCGC TTCTCCAGGA CCACAGACTG CACCAGCCGC GAAGCCTGAG 
AAGGCCTCGG CCGGCGCCCG GCTTCCTGTG CAGGAGAAGA CGAACCTGGC CGCCTACGTG 
CCCCTGCTGA CCCAGGGCTG GGCGGAGATC CTGGTCCGGA GGCCCACAGG GAACACCAGC 
TGGCTGATGA GCCTGGAGAA CCCGCTCAGC CCTTTCTCCT CGGACATCAA CAACATGCCC 
CTGCAGGAGC TGTCCAACGC CCTCATGGCA GCTGAGCGCT TCAAGGAGCA CCGGGACACA 
GCCCTGTACA AGTCGCTGTC GGTGCCAGCA GCCAGCACGG CCAAACCCCC TCCTCTGCCT 
CGCTCCAACA CAGACTCCGC CGTGGTCATG GAGGAGGGAA GTCCTGGCGA GGTTCCTGTG 
CCAGTGGAGC CCCCAGGATT GGAGGACTTT GAGGCGGCGC TAGGCGTGGA CAGGCGCACA 
GACGCCTACA GCAGGTCGTC CTCAACCTCC AGCCAGGAGG AGAAGTCATT CCACGCAGAG 
GAGCTGGTTG CCGGGGGAAT CCCCATTGAA CGAGCCGTCT CCTCGGAGGG TGGCCGGCCC 
TCTGTGGACC TCTCCTTCCA GCCCTCACAG CCCCTGAGCA AGTCTAGCTC CTCTCCTGAG 
CTGCAGACCC TGCAGGACAT CCTCGGGGAC CCTGGGGACA AGGCTGACGT GGGCCGGCTG 
AGCCCTGAGG TTAAGGCCCG TTCACAGTCA GGGATCCTGG ACGGGGAAAG TGCTGCCTGG 
CCAGCCTCGG GCGAAGACAG TCGGGGCCAG CCCGAGGGTC CCTTGCCTTC CAGCTCCCCC 
CGCTCACCCA GTGGCCTCCG GCCCCGCGGT TACACCATCT CCGACTCGGC CCCATCACGC 
AGGGGCAGGA GGGTAGAAAG GGATGCCTTT AAGAGCAGAG CCACAGCTTC CAATGCAGAG 
AAAGTGCCAG GCATCAACCC CAGTTTCGTG TTCCTGCAGC TCTACCATTC GCCTTTCTTT 
GGCGACGAGT CAAACAAGCC AATCCTGCTG CCCAATGAGT CACAGTCCTT TGAGCGGTCG 
GTGCAGCTCC TCGACCAGAT CCCATCATAC GACACCCACA AGATCGCCGT CCTGTATGTG 
GGAGAAGGCC AGAGCAACAG TGAGCTCGCC ATCCTGTCCA ATGAGCATGG CTCCTACAGG 
TACACGGAGT TCCTGACGGG CCTGGGCCGG CTCATCGAGC TCAAGGACTG CCAGCCAGAC 
AAGGTGTACC TGGGCGGCCT GGACGTGTGT GGTGAGGACG GCCAGTTCAC CTACTGCTGG 
CACGACGACA TCATGCAAGC CGTCTTCCAC ATCGCCACCC TGATGCCCAC CAAGGACGTG 
GACAAGCATC GTTGCGACAA GAAGCGCCAC CTGGGCAACG ATTTCGTGTC CATTGTCTAC 
AATGACTCTG GCGAGGACTT CAAGCTCGGC ACCATCAAGG GCCAGTTCAA CTTTGTCCAC 
GTGATCATCA CCCCGCTGGA TTATGAGTGC AACCTGGTGT CCCTGCAGTG CAGGAAAGAC 
ATGGAGGGCC TTGTGGACAC CAGCGTGGCC AAGATCGTGT CTGACCGCAA CCTGCCTTTT 
GTGGCCCGTC AGATGGCCCT GCATGCAAAC ATGGCCTCAC AGGTGCACCA CAGCCGCTCC 
AACCCCACCG ACATCTACCC CTCCAAGTGG ATTGCCCGGC TCCGCCACAT CAAGAGACTC 
CGCCAGCGGA TCTGCGAGGA AGCCGCCTAC TCCAACCCCA GCCTGCCTCT GGTGCACCCC 
CCATCCCATA CCAAAGGGCC TGCACAGACT CCAGCCGAGC CCACGCCTGG CTACGAGATG 
GGCCAGCGGA AGCGCCTCAT CTCCTCTGTG GAGGACTTCA CCGAGTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014980843.2
CDS	204..5837
Translation	MLRSGRGATCCSFSPPGRRGLLPTPPRSSPGSQAPVPRQHPGAQGVHAGLLRTTHPGGPRGPRHVTQGSPAFPLEGCLELQLPAGSVAALRAAPLPEVRVPLPAASGARGVRLSPRRGGPARGGAAREGCSGASWPTMAKPASKDSGLKEKFRILFGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLSNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIIQGQGERLGVLRALFFKVIKDYPSNEDLHERLDVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTDSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKGPAQTPAEPTPGYEMGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_015125357.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015125357.2

ORF Insert Sequence:

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAGGCTCCGT GGCGGCCCTC CGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
CCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAGCAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACCGCCGAA 
ATCCTGAGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTATATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCGTCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCGGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGGA TACAGACGTC CCTCACCAGT GCCAGCCTGG GGTCTGCAGA CGAGAACTCC 
GTGGCCCAGG CTGACGATAG CCTGAAAAGC CTCCATCTGG AGCTCACGGA AACCTGTCTG 
GACATGATGG CCCGATACGT CTTCTCCAAC TTCACAGCTG TCCCGAAGAG GTCTCCTGTG 
GGCGAGTTCC TCCTGGCGGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACTGTGACGA CGAGTGTGGG AACCGGGACC CGGTCGTTAC TAGGCCTGGA CTCGGGGGAG 
CTGCAGTCCG GCCCGGAGTC GAGCTCCAGC CCCGGCATGA ATGTGAGACA GACGAAGGAG 
GCGCCAGCCA AGCTGGAGTC CCAGGCTGGG CAGCAGGTGT CCCGTGGGGC CCGGGACCGG 
GTCCGTTCTA TGTCAGGGGG CCATGGTCTT CGAGTTGGCG CCCTGGATGT GCCGGCCTCC 
CAGTTCCCGG GCAGTGCCGC TTCTCCAGGA CCACAGACTG CACCAGCCGC GAAGCCTGAG 
AAGGCCTCGG CCGGCGCCCG GCTTCCTGTG CAGGAGAAGA CGAACCTGGC CGCCTACGTG 
CCCCTGCTGA CCCAGGGCTG GGCGGAGATC CTGGTCCGGA GGCCCACAGG GAACACCAGC 
TGGCTGATGA GCCTGGAGAA CCCGCTCAGC CCTTTCTCCT CGGACATCAA CAACATGCCC 
CTGCAGGAGC TGTCCAACGC CCTCATGGCA GCTGAGCGCT TCAAGGAGCA CCGGGACACA 
GCCCTGTACA AGTCGCTGTC GGTGCCAGCA GCCAGCACGG CCAAACCCCC TCCTCTGCCT 
CGCTCCAACA CAGACTCCGC CGTGGTCATG GAGGAGGGAA GTCCTGGCGA GGTTCCTGTG 
CCAGTGGAGC CCCCAGGATT GGAGGACTTT GAGGCGGCGC TAGGCGTGGA CAGGCGCACA 
GACGCCTACA GCAGGTCGTC CTCAACCTCC AGCCAGGAGG AGAAGTCATT CCACGCAGAG 
GAGCTGGTTG CCGGGGGAAT CCCCATTGAA CGAGCCGTCT CCTCGGAGGG TGGCCGGCCC 
TCTGTGGACC TCTCCTTCCA GCCCTCACAG CCCCTGAGCA AGTCTAGCTC CTCTCCTGAG 
CTGCAGACCC TGCAGGACAT CCTCGGGGAC CCTGGGGACA AGGCTGACGT GGGCCGGCTG 
AGCCCTGAGG TTAAGGCCCG TTCACAGTCA GGGATCCTGG ACGGGGAAAG TGCTGCCTGG 
CCAGCCTCGG GCGAAGACAG TCGGGGCCAG CCCGAGGGTC CCTTGCCTTC CAGCTCCCCC 
CGCTCACCCA GTGGCCTCCG GCCCCGCGGT TACACCATCT CCGACTCGGC CCCATCACGC 
AGGGGCAGGA GGGTAGAAAG GGATGCCTTT AAGAGCAGAG CCACAGCTTC CAATGCAGAG 
AAAGTGCCAG GCATCAACCC CAGTTTCGTG TTCCTGCAGC TCTACCATTC GCCTTTCTTT 
GGCGACGAGT CAAACAAGCC AATCCTGCTG CCCAATGAGT CACAGTCCTT TGAGCGGTCG 
GTGCAGCTCC TCGACCAGAT CCCATCATAC GACACCCACA AGATCGCCGT CCTGTATGTG 
GGAGAAGGCC AGAGCAACAG TGAGCTCGCC ATCCTGTCCA ATGAGCATGG CTCCTACAGG 
TACACGGAGT TCCTGACGGG CCTGGGCCGG CTCATCGAGC TCAAGGACTG CCAGCCAGAC 
AAGGTGTACC TGGGCGGCCT GGACGTGTGT GGTGAGGACG GCCAGTTCAC CTACTGCTGG 
CACGACGACA TCATGCAAGC CGTCTTCCAC ATCGCCACCC TGATGCCCAC CAAGGACGTG 
GACAAGCATC GTTGCGACAA GAAGCGCCAC CTGGGCAACG ATTTCGTGTC CATTGTCTAC 
AATGACTCTG GCGAGGACTT CAAGCTCGGC ACCATCAAGG GCCAGTTCAA CTTTGTCCAC 
GTGATCATCA CCCCGCTGGA TTATGAGTGC AACCTGGTGT CCCTGCAGTG CAGGAAAGAC 
ATGGAGGGCC TTGTGGACAC CAGCGTGGCC AAGATCGTGT CTGACCGCAA CCTGCCTTTT 
GTGGCCCGTC AGATGGCCCT GCATGCAAAC ATGGCCTCAC AGGTGCACCA CAGCCGCTCC 
AACCCCACCG ACATCTACCC CTCCAAGTGG ATTGCCCGGC TCCGCCACAT CAAGAGACTC 
CGCCAGCGGA TCTGCGAGGA AGCCGCCTAC TCCAACCCCA GCCTGCCTCT GGTGCACCCC 
CCATCCCATA CCAAAGGGCC TGCACAGACT CCAGCCGAGC CCACGCCTGG CTACGAGATG 
GGCCAGCGGA AGCGCCTCAT CTCCTCTGTG GAGGACTTCA CCGAGTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_028841484.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028697317.1 Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X1, mRNA.

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CloneID	OMb81164
Clone ID Related Accession (Same CDS sequence)	XM_028841484.1
Accession Version	XM_028841484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAGGCTCCGT GGCGGCCCTC CGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
CCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAGCAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACCGCCGAA 
ATCCTGAGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTATATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCGTCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCGGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGTC TGAGGATAGC CAGACCCCCC AAACAAGGCT TGAATAACTC TCCACCCGTG 
AAAGAATTCA AGGAGAGCTC TGCAGCCGAG GCCTTCCGGT GCCGCAGCAT CAGTGTGTCT 
GAACATGTGG TCCGCAGCAG GATACAGACG TCCCTCACCA GTGCCAGCCT GGGGTCTGCA 
GACGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA GCCTCCATCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCCCGATAC GTCTTCTCCA ACTTCACAGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTGGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGTGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGCAT GAATGTGAGA 
CAGACGAAGG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGTTC TATGTCAGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAT 
GTGCCGGCCT CCCAGTTCCC GGGCAGTGCC GCTTCTCCAG GACCACAGAC TGCACCAGCC 
GCGAAGCCTG AGAAGGCCTC GGCCGGCGCC CGGCTTCCTG TGCAGGAGAA GACGAACCTG 
GCCGCCTACG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CAGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCGCTG TCGGTGCCAG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCCG CCGTGGTCAT GGAGGAGGGA 
AGTCCTGGCG AGGTTCCTGT GCCAGTGGAG CCCCCAGGAT TGGAGGACTT TGAGGCGGCG 
CTAGGCGTGG ACAGGCGCAC AGACGCCTAC AGCAGGTCGT CCTCAACCTC CAGCCAGGAG 
GAGAAGTCAT TCCACGCAGA GGAGCTGGTT GCCGGGGGAA TCCCCATTGA ACGAGCCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCACA GCCCCTGAGC 
AAGTCTAGCT CCTCTCCTGA GCTGCAGACC CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCTGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GTTCACAGTC AGGGATCCTG 
GACGGGGAAA GTGCTGCCTG GCCAGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCACCC AGTGGCCTCC GGCCCCGCGG TTACACCATC 
TCCGACTCGG CCCCATCACG CAGGGGCAGG AGGGTAGAAA GGGATGCCTT TAAGAGCAGA 
GCCACAGCTT CCAATGCAGA GAAAGTGCCA GGCATCAACC CCAGGTGGGC CTCTTGCTTC 
CGGGCGGGGC TCCTGATACC ATCCTGCAGG AACCTGGTGC CTCAGTTGCC CCAGGCCGAG 
CAGGCTGGGG AGGGTCCTCG CCTGTGCCCT AGGGCTGGCT GGAGCCCCTG GCAAGGCGGT 
GGAGTGGGAG ATGGCCAGGT TCTGTGTTCC TCCCTGGGCT GTGGCTGCCC TGGCCAGGCC 
TTCACCTGGG TGCCCACTGT CTCCTCCCTG TGCAGTTTCG TGTTCCTGCA GCTCTACCAT 
TCGCCTTTCT TTGGCGACGA GTCAAACAAG CCAATCCTGC TGCCCAATGA GTCACAGTCC 
TTTGAGCGGT CGGTGCAGCT CCTCGACCAG ATCCCATCAT ACGACACCCA CAAGATCGCC 
GTCCTGTATG TGGGAGAAGG CCAGAGCAAC AGTGAGCTCG CCATCCTGTC CAATGAGCAT 
GGCTCCTACA GGTACACGGA GTTCCTGACG GGCCTGGGCC GGCTCATCGA GCTCAAGGAC 
TGCCAGCCAG ACAAGGTGTA CCTGGGCGGC CTGGACGTGT GTGGTGAGGA CGGCCAGTTC 
ACCTACTGCT GGCACGACGA CATCATGCAA GCCGTCTTCC ACATCGCCAC CCTGATGCCC 
ACCAAGGACG TGGACAAGCA TCGTTGCGAC AAGAAGCGCC ACCTGGGCAA CGATTTCGTG 
TCCATTGTCT ACAATGACTC TGGCGAGGAC TTCAAGCTCG GCACCATCAA GGGCCAGTTC 
AACTTTGTCC ACGTGATCAT CACCCCGCTG GATTATGAGT GCAACCTGGT GTCCCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAGATCGT GTCTGACCGC 
AACCTGCCTT TTGTGGCCCG TCAGATGGCC CTGCATGCAA ACATGGCCTC ACAGGTGCAC 
CACAGCCGCT CCAACCCCAC CGACATCTAC CCCTCCAAGT GGATTGCCCG GCTCCGCCAC 
ATCAAGAGAC TCCGCCAGCG GATCTGCGAG GAAGCCGCCT ACTCCAACCC CAGCCTGCCT 
CTGGTGCACC CCCCATCCCA TACCAAAGGG CCTGCACAGA CTCCAGCCGA GCCCACGCCT 
GGCTACGAGA TGGGCCAGCG GAAGCGCCTC ATCTCCTCTG TGGAGGACTT CACCGAGTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028697317.1
CDS	204..6269
Translation	MLRSGRGATCCSFSPPGRRGLLPTPPRSSPGSQAPVPRQHPGAQGVHAGLLRTTHPGGPRGPRHVTQGSPAFPLEGCLELQLPAGSVAALRAAPLPEVRVPLPAASGARGVRLSPRRGGPARGGAAREGCSGASWPTMAKPASKDSGLKEKFRILFGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLSNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIIQGQGERLGVLRALFFKVIKDYPSNEDLHERLDVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASNAEKVPGINPRWASCFRAGLLIPSCRNLVPQLPQAEQAGEGPRLCPRAGWSPWQGGGVGDGQVLCSSLGCGCPGQAFTWVPTVSSLCSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKGPAQTPAEPTPGYEMGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_028841484.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028841484.1

ORF Insert Sequence:

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAGGCTCCGT GGCGGCCCTC CGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
CCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAGCAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACCGCCGAA 
ATCCTGAGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTATATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCGTCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCGGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGTC TGAGGATAGC CAGACCCCCC AAACAAGGCT TGAATAACTC TCCACCCGTG 
AAAGAATTCA AGGAGAGCTC TGCAGCCGAG GCCTTCCGGT GCCGCAGCAT CAGTGTGTCT 
GAACATGTGG TCCGCAGCAG GATACAGACG TCCCTCACCA GTGCCAGCCT GGGGTCTGCA 
GACGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA GCCTCCATCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCCCGATAC GTCTTCTCCA ACTTCACAGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTGGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGTGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGCAT GAATGTGAGA 
CAGACGAAGG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGTTC TATGTCAGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAT 
GTGCCGGCCT CCCAGTTCCC GGGCAGTGCC GCTTCTCCAG GACCACAGAC TGCACCAGCC 
GCGAAGCCTG AGAAGGCCTC GGCCGGCGCC CGGCTTCCTG TGCAGGAGAA GACGAACCTG 
GCCGCCTACG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CAGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCGCTG TCGGTGCCAG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCCG CCGTGGTCAT GGAGGAGGGA 
AGTCCTGGCG AGGTTCCTGT GCCAGTGGAG CCCCCAGGAT TGGAGGACTT TGAGGCGGCG 
CTAGGCGTGG ACAGGCGCAC AGACGCCTAC AGCAGGTCGT CCTCAACCTC CAGCCAGGAG 
GAGAAGTCAT TCCACGCAGA GGAGCTGGTT GCCGGGGGAA TCCCCATTGA ACGAGCCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCACA GCCCCTGAGC 
AAGTCTAGCT CCTCTCCTGA GCTGCAGACC CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCTGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GTTCACAGTC AGGGATCCTG 
GACGGGGAAA GTGCTGCCTG GCCAGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCACCC AGTGGCCTCC GGCCCCGCGG TTACACCATC 
TCCGACTCGG CCCCATCACG CAGGGGCAGG AGGGTAGAAA GGGATGCCTT TAAGAGCAGA 
GCCACAGCTT CCAATGCAGA GAAAGTGCCA GGCATCAACC CCAGGTGGGC CTCTTGCTTC 
CGGGCGGGGC TCCTGATACC ATCCTGCAGG AACCTGGTGC CTCAGTTGCC CCAGGCCGAG 
CAGGCTGGGG AGGGTCCTCG CCTGTGCCCT AGGGCTGGCT GGAGCCCCTG GCAAGGCGGT 
GGAGTGGGAG ATGGCCAGGT TCTGTGTTCC TCCCTGGGCT GTGGCTGCCC TGGCCAGGCC 
TTCACCTGGG TGCCCACTGT CTCCTCCCTG TGCAGTTTCG TGTTCCTGCA GCTCTACCAT 
TCGCCTTTCT TTGGCGACGA GTCAAACAAG CCAATCCTGC TGCCCAATGA GTCACAGTCC 
TTTGAGCGGT CGGTGCAGCT CCTCGACCAG ATCCCATCAT ACGACACCCA CAAGATCGCC 
GTCCTGTATG TGGGAGAAGG CCAGAGCAAC AGTGAGCTCG CCATCCTGTC CAATGAGCAT 
GGCTCCTACA GGTACACGGA GTTCCTGACG GGCCTGGGCC GGCTCATCGA GCTCAAGGAC 
TGCCAGCCAG ACAAGGTGTA CCTGGGCGGC CTGGACGTGT GTGGTGAGGA CGGCCAGTTC 
ACCTACTGCT GGCACGACGA CATCATGCAA GCCGTCTTCC ACATCGCCAC CCTGATGCCC 
ACCAAGGACG TGGACAAGCA TCGTTGCGAC AAGAAGCGCC ACCTGGGCAA CGATTTCGTG 
TCCATTGTCT ACAATGACTC TGGCGAGGAC TTCAAGCTCG GCACCATCAA GGGCCAGTTC 
AACTTTGTCC ACGTGATCAT CACCCCGCTG GATTATGAGT GCAACCTGGT GTCCCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAGATCGT GTCTGACCGC 
AACCTGCCTT TTGTGGCCCG TCAGATGGCC CTGCATGCAA ACATGGCCTC ACAGGTGCAC 
CACAGCCGCT CCAACCCCAC CGACATCTAC CCCTCCAAGT GGATTGCCCG GCTCCGCCAC 
ATCAAGAGAC TCCGCCAGCG GATCTGCGAG GAAGCCGCCT ACTCCAACCC CAGCCTGCCT 
CTGGTGCACC CCCCATCCCA TACCAAAGGG CCTGCACAGA CTCCAGCCGA GCCCACGCCT 
GGCTACGAGA TGGGCCAGCG GAAGCGCCTC ATCTCCTCTG TGGAGGACTT CACCGAGTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_028841485.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028697318.1 Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X2, mRNA.

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CloneID	OMb81165
Clone ID Related Accession (Same CDS sequence)	XM_028841485.1
Accession Version	XM_028841485.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAGGCTCCGT GGCGGCCCTC CGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
CCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAGCAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACCGCCGAA 
ATCCTGAGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTATATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCGTCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCGGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGTC TGAGGATAGC CAGACCCCCC AAACAAGGCT TGAATAACTC TCCACCCGTG 
AAAGAATTCA AGGAGAGCTC TGCAGCCGAG GCCTTCCGGT GCCGCAGCAT CAGTGTGTCT 
GAACATGTGG TCCGCAGGAT ACAGACGTCC CTCACCAGTG CCAGCCTGGG GTCTGCAGAC 
GAGAACTCCG TGGCCCAGGC TGACGATAGC CTGAAAAGCC TCCATCTGGA GCTCACGGAA 
ACCTGTCTGG ACATGATGGC CCGATACGTC TTCTCCAACT TCACAGCTGT CCCGAAGAGG 
TCTCCTGTGG GCGAGTTCCT CCTGGCGGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CTGTGACGAC GAGTGTGGGA ACCGGGACCC GGTCGTTACT AGGCCTGGAC 
TCGGGGGAGC TGCAGTCCGG CCCGGAGTCG AGCTCCAGCC CCGGCATGAA TGTGAGACAG 
ACGAAGGAGG CGCCAGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGACCGGG TCCGTTCTAT GTCAGGGGGC CATGGTCTTC GAGTTGGCGC CCTGGATGTG 
CCGGCCTCCC AGTTCCCGGG CAGTGCCGCT TCTCCAGGAC CACAGACTGC ACCAGCCGCG 
AAGCCTGAGA AGGCCTCGGC CGGCGCCCGG CTTCCTGTGC AGGAGAAGAC GAACCTGGCC 
GCCTACGTGC CCCTGCTGAC CCAGGGCTGG GCGGAGATCC TGGTCCGGAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CTTTCTCCTC GGACATCAAC 
AACATGCCCC TGCAGGAGCT GTCCAACGCC CTCATGGCAG CTGAGCGCTT CAAGGAGCAC 
CGGGACACAG CCCTGTACAA GTCGCTGTCG GTGCCAGCAG CCAGCACGGC CAAACCCCCT 
CCTCTGCCTC GCTCCAACAC AGTGGCCTCT TTCTCCTCCC TGTACCAGTC CAGCTGCCAA 
GGACAGCTGC ACAGGAGCGT TTCCTGGGCA GACTCCGCCG TGGTCATGGA GGAGGGAAGT 
CCTGGCGAGG TTCCTGTGCC AGTGGAGCCC CCAGGATTGG AGGACTTTGA GGCGGCGCTA 
GGCGTGGACA GGCGCACAGA CGCCTACAGC AGGTCGTCCT CAACCTCCAG CCAGGAGGAG 
AAGTCATTCC ACGCAGAGGA GCTGGTTGCC GGGGGAATCC CCATTGAACG AGCCGTCTCC 
TCGGAGGGTG GCCGGCCCTC TGTGGACCTC TCCTTCCAGC CCTCACAGCC CCTGAGCAAG 
TCTAGCTCCT CTCCTGAGCT GCAGACCCTG CAGGACATCC TCGGGGACCC TGGGGACAAG 
GCTGACGTGG GCCGGCTGAG CCCTGAGGTT AAGGCCCGTT CACAGTCAGG GATCCTGGAC 
GGGGAAAGTG CTGCCTGGCC AGCCTCGGGC GAAGACAGTC GGGGCCAGCC CGAGGGTCCC 
TTGCCTTCCA GCTCCCCCCG CTCACCCAGT GGCCTCCGGC CCCGCGGTTA CACCATCTCC 
GACTCGGCCC CATCACGCAG GGGCAGGAGG GTAGAAAGGG ATGCCTTTAA GAGCAGAGCC 
ACAGCTTCCA ATGCAGAGAA AGTGCCAGGC ATCAACCCCA GGTGGGCCTC TTGCTTCCGG 
GCGGGGCTCC TGATACCATC CTGCAGGAAC CTGGTGCCTC AGTTGCCCCA GGCCGAGCAG 
GCTGGGGAGG GTCCTCGCCT GTGCCCTAGG GCTGGCTGGA GCCCCTGGCA AGGCGGTGGA 
GTGGGAGATG GCCAGGTTCT GTGTTCCTCC CTGGGCTGTG GCTGCCCTGG CCAGGCCTTC 
ACCTGGGTGC CCACTGTCTC CTCCCTGTGC AGTTTCGTGT TCCTGCAGCT CTACCATTCG 
CCTTTCTTTG GCGACGAGTC AAACAAGCCA ATCCTGCTGC CCAATGAGTC ACAGTCCTTT 
GAGCGGTCGG TGCAGCTCCT CGACCAGATC CCATCATACG ACACCCACAA GATCGCCGTC 
CTGTATGTGG GAGAAGGCCA GAGCAACAGT GAGCTCGCCA TCCTGTCCAA TGAGCATGGC 
TCCTACAGGT ACACGGAGTT CCTGACGGGC CTGGGCCGGC TCATCGAGCT CAAGGACTGC 
CAGCCAGACA AGGTGTACCT GGGCGGCCTG GACGTGTGTG GTGAGGACGG CCAGTTCACC 
TACTGCTGGC ACGACGACAT CATGCAAGCC GTCTTCCACA TCGCCACCCT GATGCCCACC 
AAGGACGTGG ACAAGCATCG TTGCGACAAG AAGCGCCACC TGGGCAACGA TTTCGTGTCC 
ATTGTCTACA ATGACTCTGG CGAGGACTTC AAGCTCGGCA CCATCAAGGG CCAGTTCAAC 
TTTGTCCACG TGATCATCAC CCCGCTGGAT TATGAGTGCA ACCTGGTGTC CCTGCAGTGC 
AGGAAAGACA TGGAGGGCCT TGTGGACACC AGCGTGGCCA AGATCGTGTC TGACCGCAAC 
CTGCCTTTTG TGGCCCGTCA GATGGCCCTG CATGCAAACA TGGCCTCACA GGTGCACCAC 
AGCCGCTCCA ACCCCACCGA CATCTACCCC TCCAAGTGGA TTGCCCGGCT CCGCCACATC 
AAGAGACTCC GCCAGCGGAT CTGCGAGGAA GCCGCCTACT CCAACCCCAG CCTGCCTCTG 
GTGCACCCCC CATCCCATAC CAAAGGGCCT GCACAGACTC CAGCCGAGCC CACGCCTGGC 
TACGAGATGG GCCAGCGGAA GCGCCTCATC TCCTCTGTGG AGGACTTCAC CGAGTTCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028697318.1
CDS	204..6266
Translation	MLRSGRGATCCSFSPPGRRGLLPTPPRSSPGSQAPVPRQHPGAQGVHAGLLRTTHPGGPRGPRHVTQGSPAFPLEGCLELQLPAGSVAALRAAPLPEVRVPLPAASGARGVRLSPRRGGPARGGAAREGCSGASWPTMAKPASKDSGLKEKFRILFGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLSNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIIQGQGERLGVLRALFFKVIKDYPSNEDLHERLDVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASNAEKVPGINPRWASCFRAGLLIPSCRNLVPQLPQAEQAGEGPRLCPRAGWSPWQGGGVGDGQVLCSSLGCGCPGQAFTWVPTVSSLCSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKGPAQTPAEPTPGYEMGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_028841485.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta TSC complex subunit 2 (TSC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028841485.1

ORF Insert Sequence:

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAGGCTCCGT GGCGGCCCTC CGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
CCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAGCAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACCGCCGAA 
ATCCTGAGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTATATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCGTCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCGGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGTC TGAGGATAGC CAGACCCCCC AAACAAGGCT TGAATAACTC TCCACCCGTG 
AAAGAATTCA AGGAGAGCTC TGCAGCCGAG GCCTTCCGGT GCCGCAGCAT CAGTGTGTCT 
GAACATGTGG TCCGCAGGAT ACAGACGTCC CTCACCAGTG CCAGCCTGGG GTCTGCAGAC 
GAGAACTCCG TGGCCCAGGC TGACGATAGC CTGAAAAGCC TCCATCTGGA GCTCACGGAA 
ACCTGTCTGG ACATGATGGC CCGATACGTC TTCTCCAACT TCACAGCTGT CCCGAAGAGG 
TCTCCTGTGG GCGAGTTCCT CCTGGCGGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CTGTGACGAC GAGTGTGGGA ACCGGGACCC GGTCGTTACT AGGCCTGGAC 
TCGGGGGAGC TGCAGTCCGG CCCGGAGTCG AGCTCCAGCC CCGGCATGAA TGTGAGACAG 
ACGAAGGAGG CGCCAGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGACCGGG TCCGTTCTAT GTCAGGGGGC CATGGTCTTC GAGTTGGCGC CCTGGATGTG 
CCGGCCTCCC AGTTCCCGGG CAGTGCCGCT TCTCCAGGAC CACAGACTGC ACCAGCCGCG 
AAGCCTGAGA AGGCCTCGGC CGGCGCCCGG CTTCCTGTGC AGGAGAAGAC GAACCTGGCC 
GCCTACGTGC CCCTGCTGAC CCAGGGCTGG GCGGAGATCC TGGTCCGGAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CTTTCTCCTC GGACATCAAC 
AACATGCCCC TGCAGGAGCT GTCCAACGCC CTCATGGCAG CTGAGCGCTT CAAGGAGCAC 
CGGGACACAG CCCTGTACAA GTCGCTGTCG GTGCCAGCAG CCAGCACGGC CAAACCCCCT 
CCTCTGCCTC GCTCCAACAC AGTGGCCTCT TTCTCCTCCC TGTACCAGTC CAGCTGCCAA 
GGACAGCTGC ACAGGAGCGT TTCCTGGGCA GACTCCGCCG TGGTCATGGA GGAGGGAAGT 
CCTGGCGAGG TTCCTGTGCC AGTGGAGCCC CCAGGATTGG AGGACTTTGA GGCGGCGCTA 
GGCGTGGACA GGCGCACAGA CGCCTACAGC AGGTCGTCCT CAACCTCCAG CCAGGAGGAG 
AAGTCATTCC ACGCAGAGGA GCTGGTTGCC GGGGGAATCC CCATTGAACG AGCCGTCTCC 
TCGGAGGGTG GCCGGCCCTC TGTGGACCTC TCCTTCCAGC CCTCACAGCC CCTGAGCAAG 
TCTAGCTCCT CTCCTGAGCT GCAGACCCTG CAGGACATCC TCGGGGACCC TGGGGACAAG 
GCTGACGTGG GCCGGCTGAG CCCTGAGGTT AAGGCCCGTT CACAGTCAGG GATCCTGGAC 
GGGGAAAGTG CTGCCTGGCC AGCCTCGGGC GAAGACAGTC GGGGCCAGCC CGAGGGTCCC 
TTGCCTTCCA GCTCCCCCCG CTCACCCAGT GGCCTCCGGC CCCGCGGTTA CACCATCTCC 
GACTCGGCCC CATCACGCAG GGGCAGGAGG GTAGAAAGGG ATGCCTTTAA GAGCAGAGCC 
ACAGCTTCCA ATGCAGAGAA AGTGCCAGGC ATCAACCCCA GGTGGGCCTC TTGCTTCCGG 
GCGGGGCTCC TGATACCATC CTGCAGGAAC CTGGTGCCTC AGTTGCCCCA GGCCGAGCAG 
GCTGGGGAGG GTCCTCGCCT GTGCCCTAGG GCTGGCTGGA GCCCCTGGCA AGGCGGTGGA 
GTGGGAGATG GCCAGGTTCT GTGTTCCTCC CTGGGCTGTG GCTGCCCTGG CCAGGCCTTC 
ACCTGGGTGC CCACTGTCTC CTCCCTGTGC AGTTTCGTGT TCCTGCAGCT CTACCATTCG 
CCTTTCTTTG GCGACGAGTC AAACAAGCCA ATCCTGCTGC CCAATGAGTC ACAGTCCTTT 
GAGCGGTCGG TGCAGCTCCT CGACCAGATC CCATCATACG ACACCCACAA GATCGCCGTC 
CTGTATGTGG GAGAAGGCCA GAGCAACAGT GAGCTCGCCA TCCTGTCCAA TGAGCATGGC 
TCCTACAGGT ACACGGAGTT CCTGACGGGC CTGGGCCGGC TCATCGAGCT CAAGGACTGC 
CAGCCAGACA AGGTGTACCT GGGCGGCCTG GACGTGTGTG GTGAGGACGG CCAGTTCACC 
TACTGCTGGC ACGACGACAT CATGCAAGCC GTCTTCCACA TCGCCACCCT GATGCCCACC 
AAGGACGTGG ACAAGCATCG TTGCGACAAG AAGCGCCACC TGGGCAACGA TTTCGTGTCC 
ATTGTCTACA ATGACTCTGG CGAGGACTTC AAGCTCGGCA CCATCAAGGG CCAGTTCAAC 
TTTGTCCACG TGATCATCAC CCCGCTGGAT TATGAGTGCA ACCTGGTGTC CCTGCAGTGC 
AGGAAAGACA TGGAGGGCCT TGTGGACACC AGCGTGGCCA AGATCGTGTC TGACCGCAAC 
CTGCCTTTTG TGGCCCGTCA GATGGCCCTG CATGCAAACA TGGCCTCACA GGTGCACCAC 
AGCCGCTCCA ACCCCACCGA CATCTACCCC TCCAAGTGGA TTGCCCGGCT CCGCCACATC 
AAGAGACTCC GCCAGCGGAT CTGCGAGGAA GCCGCCTACT CCAACCCCAG CCTGCCTCTG 
GTGCACCCCC CATCCCATAC CAAAGGGCCT GCACAGACTC CAGCCGAGCC CACGCCTGGC 
TACGAGATGG GCCAGCGGAA GCGCCTCATC TCCTCTGTGG AGGACTTCAC CGAGTTCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015125349.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014980835.1 Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.

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CloneID	OMb54728
Clone ID Related Accession (Same CDS sequence)	XM_015125349.1
Accession Version	XM_015125349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805823.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAAGCTCCGT GGCGGCCCTC TGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
GCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAACAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACGGCCGAA 
ATCCTGCGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTACATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCATCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCAGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGTC TGAGGATAGC CAGACCCCCC AAACAAGGCT TGAATAACTC TCCACCCGTG 
AAAGAATTCA AGGAGAGCTC TGCAGCCGAG GCCTTCCGGT GCCGCAGCAT CAGTGTGTCT 
GAACATGTGG TCCGCAGCAG GATACAGACA TCCCTCACCA GTGCCAGCCT GGGGTCTGCA 
GACGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA GCCTCCATCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCCCGATAC GTCTTCTCCA ACTTCACAGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTGGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGTGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGCAT GAATGTGAGA 
CAGACGAAGG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGTTC TATGTCAGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAT 
GTGCCGGCCT CCCAGTTCCC GGGCAGTGCC GCTTCTCCAG GACCACAGAC TGCACCAGCC 
GCGAAGCCTG AGAAGGCCTC GGCCGGCGCC CGGCTTCCTG TGCAGGAGAA GACGAACCTG 
GCCGCCTACG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CAGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCGCTG TCGGTGCCAG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCCG CCGTGGTCAT GGAGGAGGGA 
AGTCCTGGCG AGGTTCCTGT GCCAGTGGAG CCCCCAGGAT TGGAGGACTT TGAGGCGGCG 
CTAGGCGTGG ACAGGCGCAC AGACGCCTAC AGCAGGTCGT CCTCAACCTC CAGCCAGGAG 
GAGAAGTCAT TCCACGCAGA GGAGCTGGTT GCCGGGGGAA TCCCCATTGA ACGAGCCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCACA GCCCCTGAGC 
AAGTCTAGCT CCTCTCCTGA GCTGCAGACC CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCTGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GTTCACAGTC AGGGATCCTG 
GACGGGGAAA GTGCTGCCTG GCCAGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCACCC AGTGGCCTCC GGCCCCGCGG TTACACCATC 
TCCGACTCGG CCCCATCACG CAGGGGCAGG AGGGTAGAAA GGGATGCCTT TAAGAGCAGA 
GCCACAGCTT CCAGTGCAGA GAAAGTGCCA GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CGCCTTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT GGGAGAAGGC CAGAGCAACA GTGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTCAAGGACT GCCAGCCAGA CAAGGTGTAC CTGGGCGGCC TGGACGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGACGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAT CGTTGCGACA AGAAGCGCCA CCTGGGCAAC 
GATTTCGTGT CCATTGTCTA CAATGACTCT GGCGAGGACT TCAAGCTCGG CACCATCAAG 
GGCCAGTTCA ACTTTGTCCA CGTGATCATC ACCCCGCTGG ATTATGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGG TGGGGCCAGG GTGGGGCCGG TGTCCCTGCA GTGCAGGAAA 
GACATGGAGG GCCTTGTGGA CACCAGCGTG GCCAAGATCG TGTCTGACCG CAACCTGCCT 
TTTGTGGCCC GTCAGATGGC CCTGCATGCA AACATGGCCT CACAGGTGCA CCACAGCCGC 
TCCAACCCCA CCGACATCTA CCCCTCCAAG TGGATTGCCC GGCTCCGCCA CATCAAGAGA 
CTCCGCCAGC GGATCTGCGA GGAAGCCGCC TACTCCAACC CCAGCCTGCC TCTGGTGCAC 
CCCCCATCCC ATACCAAAGG GCCTGCACAG ACTCCAGCCG AGCCCACGCC TGGCTACGAG 
ATGGGCCAGC GGAAGCGCCT CATCTCCTCT GTGGAGGACT TCACCGAGTT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014980835.1
CDS	239..6115
Translation	MLRSGRGATCCSFSPPGRRGLLPTPPRSSPGSQAPVPRQHPGAQGVHAGLLRTTHPGGPRGPRHVTQGSPAFPLEGCLELQLPASSVAALCAAPLPEVRVALPAASGARGVRLSPRRGGPARGGAAREGCSGASWPTMAKPTSKDSGLKEKFRILFGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLSNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIIQGQGERLGVLRALFFKVIKDYPSNEDLHERLDVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCICDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKGGARVGPVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKGPAQTPAEPTPGYEMGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_015125349.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015125349.1

ORF Insert Sequence:

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAAGCTCCGT GGCGGCCCTC TGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
GCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAACAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACGGCCGAA 
ATCCTGCGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTACATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCATCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCAGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGTC TGAGGATAGC CAGACCCCCC AAACAAGGCT TGAATAACTC TCCACCCGTG 
AAAGAATTCA AGGAGAGCTC TGCAGCCGAG GCCTTCCGGT GCCGCAGCAT CAGTGTGTCT 
GAACATGTGG TCCGCAGCAG GATACAGACA TCCCTCACCA GTGCCAGCCT GGGGTCTGCA 
GACGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA GCCTCCATCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCCCGATAC GTCTTCTCCA ACTTCACAGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTGGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGTGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGCAT GAATGTGAGA 
CAGACGAAGG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGTTC TATGTCAGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAT 
GTGCCGGCCT CCCAGTTCCC GGGCAGTGCC GCTTCTCCAG GACCACAGAC TGCACCAGCC 
GCGAAGCCTG AGAAGGCCTC GGCCGGCGCC CGGCTTCCTG TGCAGGAGAA GACGAACCTG 
GCCGCCTACG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CAGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCGCTG TCGGTGCCAG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCCG CCGTGGTCAT GGAGGAGGGA 
AGTCCTGGCG AGGTTCCTGT GCCAGTGGAG CCCCCAGGAT TGGAGGACTT TGAGGCGGCG 
CTAGGCGTGG ACAGGCGCAC AGACGCCTAC AGCAGGTCGT CCTCAACCTC CAGCCAGGAG 
GAGAAGTCAT TCCACGCAGA GGAGCTGGTT GCCGGGGGAA TCCCCATTGA ACGAGCCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCACA GCCCCTGAGC 
AAGTCTAGCT CCTCTCCTGA GCTGCAGACC CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCTGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GTTCACAGTC AGGGATCCTG 
GACGGGGAAA GTGCTGCCTG GCCAGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCACCC AGTGGCCTCC GGCCCCGCGG TTACACCATC 
TCCGACTCGG CCCCATCACG CAGGGGCAGG AGGGTAGAAA GGGATGCCTT TAAGAGCAGA 
GCCACAGCTT CCAGTGCAGA GAAAGTGCCA GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CGCCTTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT GGGAGAAGGC CAGAGCAACA GTGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTCAAGGACT GCCAGCCAGA CAAGGTGTAC CTGGGCGGCC TGGACGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGACGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAT CGTTGCGACA AGAAGCGCCA CCTGGGCAAC 
GATTTCGTGT CCATTGTCTA CAATGACTCT GGCGAGGACT TCAAGCTCGG CACCATCAAG 
GGCCAGTTCA ACTTTGTCCA CGTGATCATC ACCCCGCTGG ATTATGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGG TGGGGCCAGG GTGGGGCCGG TGTCCCTGCA GTGCAGGAAA 
GACATGGAGG GCCTTGTGGA CACCAGCGTG GCCAAGATCG TGTCTGACCG CAACCTGCCT 
TTTGTGGCCC GTCAGATGGC CCTGCATGCA AACATGGCCT CACAGGTGCA CCACAGCCGC 
TCCAACCCCA CCGACATCTA CCCCTCCAAG TGGATTGCCC GGCTCCGCCA CATCAAGAGA 
CTCCGCCAGC GGATCTGCGA GGAAGCCGCC TACTCCAACC CCAGCCTGCC TCTGGTGCAC 
CCCCCATCCC ATACCAAAGG GCCTGCACAG ACTCCAGCCG AGCCCACGCC TGGCTACGAG 
ATGGGCCAGC GGAAGCGCCT CATCTCCTCT GTGGAGGACT TCACCGAGTT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015125350.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014980836.1 Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.

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CloneID	OMb54729
Clone ID Related Accession (Same CDS sequence)	XM_015125350.1
Accession Version	XM_015125350.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805823.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAAGCTCCGT GGCGGCCCTC TGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
GCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAACAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACGGCCGAA 
ATCCTGCGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTACATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCATCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCAGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGTC TGAGGATAGC CAGACCCCCC AAACAAGGCT TGAATAACTC TCCACCCGTG 
AAAGAATTCA AGGAGAGCTC TGCAGCCGAG GCCTTCCGGT GCCGCAGCAT CAGTGTGTCT 
GAACATGTGG TCCGCAGGAT ACAGACATCC CTCACCAGTG CCAGCCTGGG GTCTGCAGAC 
GAGAACTCCG TGGCCCAGGC TGACGATAGC CTGAAAAGCC TCCATCTGGA GCTCACGGAA 
ACCTGTCTGG ACATGATGGC CCGATACGTC TTCTCCAACT TCACAGCTGT CCCGAAGAGG 
TCTCCTGTGG GCGAGTTCCT CCTGGCGGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CTGTGACGAC GAGTGTGGGA ACCGGGACCC GGTCGTTACT AGGCCTGGAC 
TCGGGGGAGC TGCAGTCCGG CCCGGAGTCG AGCTCCAGCC CCGGCATGAA TGTGAGACAG 
ACGAAGGAGG CGCCAGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGACCGGG TCCGTTCTAT GTCAGGGGGC CATGGTCTTC GAGTTGGCGC CCTGGATGTG 
CCGGCCTCCC AGTTCCCGGG CAGTGCCGCT TCTCCAGGAC CACAGACTGC ACCAGCCGCG 
AAGCCTGAGA AGGCCTCGGC CGGCGCCCGG CTTCCTGTGC AGGAGAAGAC GAACCTGGCC 
GCCTACGTGC CCCTGCTGAC CCAGGGCTGG GCGGAGATCC TGGTCCGGAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CTTTCTCCTC GGACATCAAC 
AACATGCCCC TGCAGGAGCT GTCCAACGCC CTCATGGCAG CTGAGCGCTT CAAGGAGCAC 
CGGGACACAG CCCTGTACAA GTCGCTGTCG GTGCCAGCAG CCAGCACGGC CAAACCCCCT 
CCTCTGCCTC GCTCCAACAC AGTGGCCTCT TTCTCCTCCC TGTACCAGTC CAGCTGCCAA 
GGACAGCTGC ACAGGAGCGT TTCCTGGGCA GACTCCGCCG TGGTCATGGA GGAGGGAAGT 
CCTGGCGAGG TTCCTGTGCC AGTGGAGCCC CCAGGATTGG AGGACTTTGA GGCGGCGCTA 
GGCGTGGACA GGCGCACAGA CGCCTACAGC AGGTCGTCCT CAACCTCCAG CCAGGAGGAG 
AAGTCATTCC ACGCAGAGGA GCTGGTTGCC GGGGGAATCC CCATTGAACG AGCCGTCTCC 
TCGGAGGGTG GCCGGCCCTC TGTGGACCTC TCCTTCCAGC CCTCACAGCC CCTGAGCAAG 
TCTAGCTCCT CTCCTGAGCT GCAGACCCTG CAGGACATCC TCGGGGACCC TGGGGACAAG 
GCTGACGTGG GCCGGCTGAG CCCTGAGGTT AAGGCCCGTT CACAGTCAGG GATCCTGGAC 
GGGGAAAGTG CTGCCTGGCC AGCCTCGGGC GAAGACAGTC GGGGCCAGCC CGAGGGTCCC 
TTGCCTTCCA GCTCCCCCCG CTCACCCAGT GGCCTCCGGC CCCGCGGTTA CACCATCTCC 
GACTCGGCCC CATCACGCAG GGGCAGGAGG GTAGAAAGGG ATGCCTTTAA GAGCAGAGCC 
ACAGCTTCCA GTGCAGAGAA AGTGCCAGGC ATCAACCCCA GTTTCGTGTT CCTGCAGCTC 
TACCATTCGC CTTTCTTTGG CGACGAGTCA AACAAGCCAA TCCTGCTGCC CAATGAGTCA 
CAGTCCTTTG AGCGGTCGGT GCAGCTCCTC GACCAGATCC CATCATACGA CACCCACAAG 
ATCGCCGTCC TGTATGTGGG AGAAGGCCAG AGCAACAGTG AGCTCGCCAT CCTGTCCAAT 
GAGCATGGCT CCTACAGGTA CACGGAGTTC CTGACGGGCC TGGGCCGGCT CATCGAGCTC 
AAGGACTGCC AGCCAGACAA GGTGTACCTG GGCGGCCTGG ACGTGTGTGG TGAGGACGGC 
CAGTTCACCT ACTGCTGGCA CGACGACATC ATGCAAGCCG TCTTCCACAT CGCCACCCTG 
ATGCCCACCA AGGACGTGGA CAAGCATCGT TGCGACAAGA AGCGCCACCT GGGCAACGAT 
TTCGTGTCCA TTGTCTACAA TGACTCTGGC GAGGACTTCA AGCTCGGCAC CATCAAGGGC 
CAGTTCAACT TTGTCCACGT GATCATCACC CCGCTGGATT ATGAGTGCAA CCTGGTGTCC 
CTGCAGTGCA GGAAAGGTGG GGCCAGGGTG GGGCCGGTGT CCCTGCAGTG CAGGAAAGAC 
ATGGAGGGCC TTGTGGACAC CAGCGTGGCC AAGATCGTGT CTGACCGCAA CCTGCCTTTT 
GTGGCCCGTC AGATGGCCCT GCATGCAAAC ATGGCCTCAC AGGTGCACCA CAGCCGCTCC 
AACCCCACCG ACATCTACCC CTCCAAGTGG ATTGCCCGGC TCCGCCACAT CAAGAGACTC 
CGCCAGCGGA TCTGCGAGGA AGCCGCCTAC TCCAACCCCA GCCTGCCTCT GGTGCACCCC 
CCATCCCATA CCAAAGGGCC TGCACAGACT CCAGCCGAGC CCACGCCTGG CTACGAGATG 
GGCCAGCGGA AGCGCCTCAT CTCCTCTGTG GAGGACTTCA CCGAGTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014980836.1
CDS	240..6113
Translation	MLRSGRGATCCSFSPPGRRGLLPTPPRSSPGSQAPVPRQHPGAQGVHAGLLRTTHPGGPRGPRHVTQGSPAFPLEGCLELQLPASSVAALCAAPLPEVRVALPAASGARGVRLSPRRGGPARGGAAREGCSGASWPTMAKPTSKDSGLKEKFRILFGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLSNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIIQGQGERLGVLRALFFKVIKDYPSNEDLHERLDVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCICDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKGGARVGPVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKGPAQTPAEPTPGYEMGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_015125350.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015125350.1

ORF Insert Sequence:

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAAGCTCCGT GGCGGCCCTC TGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
GCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAACAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACGGCCGAA 
ATCCTGCGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTACATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCATCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCAGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGTC TGAGGATAGC CAGACCCCCC AAACAAGGCT TGAATAACTC TCCACCCGTG 
AAAGAATTCA AGGAGAGCTC TGCAGCCGAG GCCTTCCGGT GCCGCAGCAT CAGTGTGTCT 
GAACATGTGG TCCGCAGGAT ACAGACATCC CTCACCAGTG CCAGCCTGGG GTCTGCAGAC 
GAGAACTCCG TGGCCCAGGC TGACGATAGC CTGAAAAGCC TCCATCTGGA GCTCACGGAA 
ACCTGTCTGG ACATGATGGC CCGATACGTC TTCTCCAACT TCACAGCTGT CCCGAAGAGG 
TCTCCTGTGG GCGAGTTCCT CCTGGCGGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CTGTGACGAC GAGTGTGGGA ACCGGGACCC GGTCGTTACT AGGCCTGGAC 
TCGGGGGAGC TGCAGTCCGG CCCGGAGTCG AGCTCCAGCC CCGGCATGAA TGTGAGACAG 
ACGAAGGAGG CGCCAGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGACCGGG TCCGTTCTAT GTCAGGGGGC CATGGTCTTC GAGTTGGCGC CCTGGATGTG 
CCGGCCTCCC AGTTCCCGGG CAGTGCCGCT TCTCCAGGAC CACAGACTGC ACCAGCCGCG 
AAGCCTGAGA AGGCCTCGGC CGGCGCCCGG CTTCCTGTGC AGGAGAAGAC GAACCTGGCC 
GCCTACGTGC CCCTGCTGAC CCAGGGCTGG GCGGAGATCC TGGTCCGGAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CTTTCTCCTC GGACATCAAC 
AACATGCCCC TGCAGGAGCT GTCCAACGCC CTCATGGCAG CTGAGCGCTT CAAGGAGCAC 
CGGGACACAG CCCTGTACAA GTCGCTGTCG GTGCCAGCAG CCAGCACGGC CAAACCCCCT 
CCTCTGCCTC GCTCCAACAC AGTGGCCTCT TTCTCCTCCC TGTACCAGTC CAGCTGCCAA 
GGACAGCTGC ACAGGAGCGT TTCCTGGGCA GACTCCGCCG TGGTCATGGA GGAGGGAAGT 
CCTGGCGAGG TTCCTGTGCC AGTGGAGCCC CCAGGATTGG AGGACTTTGA GGCGGCGCTA 
GGCGTGGACA GGCGCACAGA CGCCTACAGC AGGTCGTCCT CAACCTCCAG CCAGGAGGAG 
AAGTCATTCC ACGCAGAGGA GCTGGTTGCC GGGGGAATCC CCATTGAACG AGCCGTCTCC 
TCGGAGGGTG GCCGGCCCTC TGTGGACCTC TCCTTCCAGC CCTCACAGCC CCTGAGCAAG 
TCTAGCTCCT CTCCTGAGCT GCAGACCCTG CAGGACATCC TCGGGGACCC TGGGGACAAG 
GCTGACGTGG GCCGGCTGAG CCCTGAGGTT AAGGCCCGTT CACAGTCAGG GATCCTGGAC 
GGGGAAAGTG CTGCCTGGCC AGCCTCGGGC GAAGACAGTC GGGGCCAGCC CGAGGGTCCC 
TTGCCTTCCA GCTCCCCCCG CTCACCCAGT GGCCTCCGGC CCCGCGGTTA CACCATCTCC 
GACTCGGCCC CATCACGCAG GGGCAGGAGG GTAGAAAGGG ATGCCTTTAA GAGCAGAGCC 
ACAGCTTCCA GTGCAGAGAA AGTGCCAGGC ATCAACCCCA GTTTCGTGTT CCTGCAGCTC 
TACCATTCGC CTTTCTTTGG CGACGAGTCA AACAAGCCAA TCCTGCTGCC CAATGAGTCA 
CAGTCCTTTG AGCGGTCGGT GCAGCTCCTC GACCAGATCC CATCATACGA CACCCACAAG 
ATCGCCGTCC TGTATGTGGG AGAAGGCCAG AGCAACAGTG AGCTCGCCAT CCTGTCCAAT 
GAGCATGGCT CCTACAGGTA CACGGAGTTC CTGACGGGCC TGGGCCGGCT CATCGAGCTC 
AAGGACTGCC AGCCAGACAA GGTGTACCTG GGCGGCCTGG ACGTGTGTGG TGAGGACGGC 
CAGTTCACCT ACTGCTGGCA CGACGACATC ATGCAAGCCG TCTTCCACAT CGCCACCCTG 
ATGCCCACCA AGGACGTGGA CAAGCATCGT TGCGACAAGA AGCGCCACCT GGGCAACGAT 
TTCGTGTCCA TTGTCTACAA TGACTCTGGC GAGGACTTCA AGCTCGGCAC CATCAAGGGC 
CAGTTCAACT TTGTCCACGT GATCATCACC CCGCTGGATT ATGAGTGCAA CCTGGTGTCC 
CTGCAGTGCA GGAAAGGTGG GGCCAGGGTG GGGCCGGTGT CCCTGCAGTG CAGGAAAGAC 
ATGGAGGGCC TTGTGGACAC CAGCGTGGCC AAGATCGTGT CTGACCGCAA CCTGCCTTTT 
GTGGCCCGTC AGATGGCCCT GCATGCAAAC ATGGCCTCAC AGGTGCACCA CAGCCGCTCC 
AACCCCACCG ACATCTACCC CTCCAAGTGG ATTGCCCGGC TCCGCCACAT CAAGAGACTC 
CGCCAGCGGA TCTGCGAGGA AGCCGCCTAC TCCAACCCCA GCCTGCCTCT GGTGCACCCC 
CCATCCCATA CCAAAGGGCC TGCACAGACT CCAGCCGAGC CCACGCCTGG CTACGAGATG 
GGCCAGCGGA AGCGCCTCAT CTCCTCTGTG GAGGACTTCA CCGAGTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_001083256.3 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_001083256.2 Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA.

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CloneID	OMb54730
Clone ID Related Accession (Same CDS sequence)	XM_001083256.3
Accession Version	XM_001083256.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805823.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 21, 2015 this sequence version replaced XM_001083256.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAAGCTCCGT GGCGGCCCTC TGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
GCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAACAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACGGCCGAA 
ATCCTGCGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTACATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCATCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCAGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGTC TGAGGATAGC CAGACCCCCC AAACAAGGCT TGAATAACTC TCCACCCGTG 
AAAGAATTCA AGGAGAGCTC TGCAGCCGAG GCCTTCCGGT GCCGCAGCAT CAGTGTGTCT 
GAACATGTGG TCCGCAGCAG GATACAGACA TCCCTCACCA GTGCCAGCCT GGGGTCTGCA 
GACGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA GCCTCCATCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCCCGATAC GTCTTCTCCA ACTTCACAGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTGGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGTGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGCAT GAATGTGAGA 
CAGACGAAGG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGTTC TATGTCAGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAT 
GTGCCGGCCT CCCAGTTCCC GGGCAGTGCC GCTTCTCCAG GACCACAGAC TGCACCAGCC 
GCGAAGCCTG AGAAGGCCTC GGCCGGCGCC CGGCTTCCTG TGCAGGAGAA GACGAACCTG 
GCCGCCTACG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CAGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCGCTG TCGGTGCCAG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCCG CCGTGGTCAT GGAGGAGGGA 
AGTCCTGGCG AGGTTCCTGT GCCAGTGGAG CCCCCAGGAT TGGAGGACTT TGAGGCGGCG 
CTAGGCGTGG ACAGGCGCAC AGACGCCTAC AGCAGGTCGT CCTCAACCTC CAGCCAGGAG 
GAGAAGTCAT TCCACGCAGA GGAGCTGGTT GCCGGGGGAA TCCCCATTGA ACGAGCCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCACA GCCCCTGAGC 
AAGTCTAGCT CCTCTCCTGA GCTGCAGACC CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCTGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GTTCACAGTC AGGGATCCTG 
GACGGGGAAA GTGCTGCCTG GCCAGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCACCC AGTGGCCTCC GGCCCCGCGG TTACACCATC 
TCCGACTCGG CCCCATCACG CAGGGGCAGG AGGGTAGAAA GGGATGCCTT TAAGAGCAGA 
GCCACAGCTT CCAGTGCAGA GAAAGTGCCA GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CGCCTTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT GGGAGAAGGC CAGAGCAACA GTGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTCAAGGACT GCCAGCCAGA CAAGGTGTAC CTGGGCGGCC TGGACGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGACGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAT CGTTGCGACA AGAAGCGCCA CCTGGGCAAC 
GATTTCGTGT CCATTGTCTA CAATGACTCT GGCGAGGACT TCAAGCTCGG CACCATCAAG 
GGCCAGTTCA ACTTTGTCCA CGTGATCATC ACCCCGCTGG ATTATGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACCTGCCTTT TGTGGCCCGT CAGATGGCCC TGCATGCAAA CATGGCCTCA 
CAGGTGCACC ACAGCCGCTC CAACCCCACC GACATCTACC CCTCCAAGTG GATTGCCCGG 
CTCCGCCACA TCAAGAGACT CCGCCAGCGG ATCTGCGAGG AAGCCGCCTA CTCCAACCCC 
AGCCTGCCTC TGGTGCACCC CCCATCCCAT ACCAAAGGGC CTGCACAGAC TCCAGCCGAG 
CCCACGCCTG GCTACGAGAT GGGCCAGCGG AAGCGCCTCA TCTCCTCTGT GGAGGACTTC 
ACCGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001083256.2
CDS	238..6072
Translation	MLRSGRGATCCSFSPPGRRGLLPTPPRSSPGSQAPVPRQHPGAQGVHAGLLRTTHPGGPRGPRHVTQGSPAFPLEGCLELQLPASSVAALCAAPLPEVRVALPAASGARGVRLSPRRGGPARGGAAREGCSGASWPTMAKPTSKDSGLKEKFRILFGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLSNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIIQGQGERLGVLRALFFKVIKDYPSNEDLHERLDVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCICDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKGPAQTPAEPTPGYEMGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_001083256.3
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001083256.3

ORF Insert Sequence:

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAAGCTCCGT GGCGGCCCTC TGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
GCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAACAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACGGCCGAA 
ATCCTGCGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTACATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCATCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCAGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGTC TGAGGATAGC CAGACCCCCC AAACAAGGCT TGAATAACTC TCCACCCGTG 
AAAGAATTCA AGGAGAGCTC TGCAGCCGAG GCCTTCCGGT GCCGCAGCAT CAGTGTGTCT 
GAACATGTGG TCCGCAGCAG GATACAGACA TCCCTCACCA GTGCCAGCCT GGGGTCTGCA 
GACGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA GCCTCCATCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCCCGATAC GTCTTCTCCA ACTTCACAGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTGGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGTGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGCAT GAATGTGAGA 
CAGACGAAGG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGTTC TATGTCAGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAT 
GTGCCGGCCT CCCAGTTCCC GGGCAGTGCC GCTTCTCCAG GACCACAGAC TGCACCAGCC 
GCGAAGCCTG AGAAGGCCTC GGCCGGCGCC CGGCTTCCTG TGCAGGAGAA GACGAACCTG 
GCCGCCTACG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CAGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCGCTG TCGGTGCCAG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCCG CCGTGGTCAT GGAGGAGGGA 
AGTCCTGGCG AGGTTCCTGT GCCAGTGGAG CCCCCAGGAT TGGAGGACTT TGAGGCGGCG 
CTAGGCGTGG ACAGGCGCAC AGACGCCTAC AGCAGGTCGT CCTCAACCTC CAGCCAGGAG 
GAGAAGTCAT TCCACGCAGA GGAGCTGGTT GCCGGGGGAA TCCCCATTGA ACGAGCCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCACA GCCCCTGAGC 
AAGTCTAGCT CCTCTCCTGA GCTGCAGACC CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCTGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GTTCACAGTC AGGGATCCTG 
GACGGGGAAA GTGCTGCCTG GCCAGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCACCC AGTGGCCTCC GGCCCCGCGG TTACACCATC 
TCCGACTCGG CCCCATCACG CAGGGGCAGG AGGGTAGAAA GGGATGCCTT TAAGAGCAGA 
GCCACAGCTT CCAGTGCAGA GAAAGTGCCA GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CGCCTTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT GGGAGAAGGC CAGAGCAACA GTGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTCAAGGACT GCCAGCCAGA CAAGGTGTAC CTGGGCGGCC TGGACGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGACGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAT CGTTGCGACA AGAAGCGCCA CCTGGGCAAC 
GATTTCGTGT CCATTGTCTA CAATGACTCT GGCGAGGACT TCAAGCTCGG CACCATCAAG 
GGCCAGTTCA ACTTTGTCCA CGTGATCATC ACCCCGCTGG ATTATGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACCTGCCTTT TGTGGCCCGT CAGATGGCCC TGCATGCAAA CATGGCCTCA 
CAGGTGCACC ACAGCCGCTC CAACCCCACC GACATCTACC CCTCCAAGTG GATTGCCCGG 
CTCCGCCACA TCAAGAGACT CCGCCAGCGG ATCTGCGAGG AAGCCGCCTA CTCCAACCCC 
AGCCTGCCTC TGGTGCACCC CCCATCCCAT ACCAAAGGGC CTGCACAGAC TCCAGCCGAG 
CCCACGCCTG GCTACGAGAT GGGCCAGCGG AAGCGCCTCA TCTCCTCTGT GGAGGACTTC 
ACCGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015125351.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014980837.1 Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA.

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CloneID	OMb54731
Clone ID Related Accession (Same CDS sequence)	XM_015125351.1
Accession Version	XM_015125351.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805823.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAAGCTCCGT GGCGGCCCTC TGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
GCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAACAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACGGCCGAA 
ATCCTGCGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTACATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCATCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCAGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGTC TGAGGATAGC CAGACCCCCC AAACAAGGCT TGAATAACTC TCCACCCGTG 
AAAGAATTCA AGGAGAGCTC TGCAGCCGAG GCCTTCCGGT GCCGCAGCAT CAGTGTGTCT 
GAACATGTGG TCCGCAGCAG GATACAGACA TCCCTCACCA GTGCCAGCCT GGGGTCTGCA 
GACGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA GCCTCCATCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCCCGATAC GTCTTCTCCA ACTTCACAGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTGGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGTGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGCAT GAATGTGAGA 
CAGACGAAGG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGTTC TATGTCAGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAT 
GTGCCGGCCT CCCAGTTCCC GGGCAGTGCC GCTTCTCCAG GACCACAGAC TGCACCAGCC 
GCGAAGCCTG AGAAGGCCTC GGCCGGCGCC CGGCTTCCTG TGCAGGAGAA GACGAACCTG 
GCCGCCTACG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CAGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCGCTG TCGGTGCCAG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGACTCC GCCGTGGTCA TGGAGGAGGG AAGTCCTGGC 
GAGGTTCCTG TGCCAGTGGA GCCCCCAGGA TTGGAGGACT TTGAGGCGGC GCTAGGCGTG 
GACAGGCGCA CAGACGCCTA CAGCAGGTCG TCCTCAACCT CCAGCCAGGA GGAGAAGTCA 
TTCCACGCAG AGGAGCTGGT TGCCGGGGGA ATCCCCATTG AACGAGCCGT CTCCTCGGAG 
GGTGGCCGGC CCTCTGTGGA CCTCTCCTTC CAGCCCTCAC AGCCCCTGAG CAAGTCTAGC 
TCCTCTCCTG AGCTGCAGAC CCTGCAGGAC ATCCTCGGGG ACCCTGGGGA CAAGGCTGAC 
GTGGGCCGGC TGAGCCCTGA GGTTAAGGCC CGTTCACAGT CAGGGATCCT GGACGGGGAA 
AGTGCTGCCT GGCCAGCCTC GGGCGAAGAC AGTCGGGGCC AGCCCGAGGG TCCCTTGCCT 
TCCAGCTCCC CCCGCTCACC CAGTGGCCTC CGGCCCCGCG GTTACACCAT CTCCGACTCG 
GCCCCATCAC GCAGGGGCAG GAGGGTAGAA AGGGATGCCT TTAAGAGCAG AGCCACAGCT 
TCCAGTGCAG AGAAAGTGCC AGGCATCAAC CCCAGTTTCG TGTTCCTGCA GCTCTACCAT 
TCGCCTTTCT TTGGCGACGA GTCAAACAAG CCAATCCTGC TGCCCAATGA GTCACAGTCC 
TTTGAGCGGT CGGTGCAGCT CCTCGACCAG ATCCCATCAT ACGACACCCA CAAGATCGCC 
GTCCTGTATG TGGGAGAAGG CCAGAGCAAC AGTGAGCTCG CCATCCTGTC CAATGAGCAT 
GGCTCCTACA GGTACACGGA GTTCCTGACG GGCCTGGGCC GGCTCATCGA GCTCAAGGAC 
TGCCAGCCAG ACAAGGTGTA CCTGGGCGGC CTGGACGTGT GTGGTGAGGA CGGCCAGTTC 
ACCTACTGCT GGCACGACGA CATCATGCAA GCCGTCTTCC ACATCGCCAC CCTGATGCCC 
ACCAAGGACG TGGACAAGCA TCGTTGCGAC AAGAAGCGCC ACCTGGGCAA CGATTTCGTG 
TCCATTGTCT ACAATGACTC TGGCGAGGAC TTCAAGCTCG GCACCATCAA GGGCCAGTTC 
AACTTTGTCC ACGTGATCAT CACCCCGCTG GATTATGAGT GCAACCTGGT GTCCCTGCAG 
TGCAGGAAAG GTGGGGCCAG GGTGGGGCCG GTGTCCCTGC AGTGCAGGAA AGACATGGAG 
GGCCTTGTGG ACACCAGCGT GGCCAAGATC GTGTCTGACC GCAACCTGCC TTTTGTGGCC 
CGTCAGATGG CCCTGCATGC AAACATGGCC TCACAGGTGC ACCACAGCCG CTCCAACCCC 
ACCGACATCT ACCCCTCCAA GTGGATTGCC CGGCTCCGCC ACATCAAGAG ACTCCGCCAG 
CGGATCTGCG AGGAAGCCGC CTACTCCAAC CCCAGCCTGC CTCTGGTGCA CCCCCCATCC 
CATACCAAAG GGCCTGCACA GACTCCAGCC GAGCCCACGC CTGGCTACGA GATGGGCCAG 
CGGAAGCGCC TCATCTCCTC TGTGGAGGAC TTCACCGAGT TCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014980837.1
CDS	239..6046
Translation	MLRSGRGATCCSFSPPGRRGLLPTPPRSSPGSQAPVPRQHPGAQGVHAGLLRTTHPGGPRGPRHVTQGSPAFPLEGCLELQLPASSVAALCAAPLPEVRVALPAASGARGVRLSPRRGGPARGGAAREGCSGASWPTMAKPTSKDSGLKEKFRILFGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLSNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIIQGQGERLGVLRALFFKVIKDYPSNEDLHERLDVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCICDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTDSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKGGARVGPVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKGPAQTPAEPTPGYEMGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_015125351.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015125351.1

ORF Insert Sequence:

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAAGCTCCGT GGCGGCCCTC TGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
GCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAACAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACGGCCGAA 
ATCCTGCGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTACATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCATCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCAGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGTC TGAGGATAGC CAGACCCCCC AAACAAGGCT TGAATAACTC TCCACCCGTG 
AAAGAATTCA AGGAGAGCTC TGCAGCCGAG GCCTTCCGGT GCCGCAGCAT CAGTGTGTCT 
GAACATGTGG TCCGCAGCAG GATACAGACA TCCCTCACCA GTGCCAGCCT GGGGTCTGCA 
GACGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA GCCTCCATCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCCCGATAC GTCTTCTCCA ACTTCACAGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTGGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGTGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGCAT GAATGTGAGA 
CAGACGAAGG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGTTC TATGTCAGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAT 
GTGCCGGCCT CCCAGTTCCC GGGCAGTGCC GCTTCTCCAG GACCACAGAC TGCACCAGCC 
GCGAAGCCTG AGAAGGCCTC GGCCGGCGCC CGGCTTCCTG TGCAGGAGAA GACGAACCTG 
GCCGCCTACG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CAGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCGCTG TCGGTGCCAG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGACTCC GCCGTGGTCA TGGAGGAGGG AAGTCCTGGC 
GAGGTTCCTG TGCCAGTGGA GCCCCCAGGA TTGGAGGACT TTGAGGCGGC GCTAGGCGTG 
GACAGGCGCA CAGACGCCTA CAGCAGGTCG TCCTCAACCT CCAGCCAGGA GGAGAAGTCA 
TTCCACGCAG AGGAGCTGGT TGCCGGGGGA ATCCCCATTG AACGAGCCGT CTCCTCGGAG 
GGTGGCCGGC CCTCTGTGGA CCTCTCCTTC CAGCCCTCAC AGCCCCTGAG CAAGTCTAGC 
TCCTCTCCTG AGCTGCAGAC CCTGCAGGAC ATCCTCGGGG ACCCTGGGGA CAAGGCTGAC 
GTGGGCCGGC TGAGCCCTGA GGTTAAGGCC CGTTCACAGT CAGGGATCCT GGACGGGGAA 
AGTGCTGCCT GGCCAGCCTC GGGCGAAGAC AGTCGGGGCC AGCCCGAGGG TCCCTTGCCT 
TCCAGCTCCC CCCGCTCACC CAGTGGCCTC CGGCCCCGCG GTTACACCAT CTCCGACTCG 
GCCCCATCAC GCAGGGGCAG GAGGGTAGAA AGGGATGCCT TTAAGAGCAG AGCCACAGCT 
TCCAGTGCAG AGAAAGTGCC AGGCATCAAC CCCAGTTTCG TGTTCCTGCA GCTCTACCAT 
TCGCCTTTCT TTGGCGACGA GTCAAACAAG CCAATCCTGC TGCCCAATGA GTCACAGTCC 
TTTGAGCGGT CGGTGCAGCT CCTCGACCAG ATCCCATCAT ACGACACCCA CAAGATCGCC 
GTCCTGTATG TGGGAGAAGG CCAGAGCAAC AGTGAGCTCG CCATCCTGTC CAATGAGCAT 
GGCTCCTACA GGTACACGGA GTTCCTGACG GGCCTGGGCC GGCTCATCGA GCTCAAGGAC 
TGCCAGCCAG ACAAGGTGTA CCTGGGCGGC CTGGACGTGT GTGGTGAGGA CGGCCAGTTC 
ACCTACTGCT GGCACGACGA CATCATGCAA GCCGTCTTCC ACATCGCCAC CCTGATGCCC 
ACCAAGGACG TGGACAAGCA TCGTTGCGAC AAGAAGCGCC ACCTGGGCAA CGATTTCGTG 
TCCATTGTCT ACAATGACTC TGGCGAGGAC TTCAAGCTCG GCACCATCAA GGGCCAGTTC 
AACTTTGTCC ACGTGATCAT CACCCCGCTG GATTATGAGT GCAACCTGGT GTCCCTGCAG 
TGCAGGAAAG GTGGGGCCAG GGTGGGGCCG GTGTCCCTGC AGTGCAGGAA AGACATGGAG 
GGCCTTGTGG ACACCAGCGT GGCCAAGATC GTGTCTGACC GCAACCTGCC TTTTGTGGCC 
CGTCAGATGG CCCTGCATGC AAACATGGCC TCACAGGTGC ACCACAGCCG CTCCAACCCC 
ACCGACATCT ACCCCTCCAA GTGGATTGCC CGGCTCCGCC ACATCAAGAG ACTCCGCCAG 
CGGATCTGCG AGGAAGCCGC CTACTCCAAC CCCAGCCTGC CTCTGGTGCA CCCCCCATCC 
CATACCAAAG GGCCTGCACA GACTCCAGCC GAGCCCACGC CTGGCTACGA GATGGGCCAG 
CGGAAGCGCC TCATCTCCTC TGTGGAGGAC TTCACCGAGT TCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015125352.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014980838.1 Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA.

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CloneID	OMb54732
Clone ID Related Accession (Same CDS sequence)	XM_015125352.1
Accession Version	XM_015125352.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805823.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAAGCTCCGT GGCGGCCCTC TGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
GCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAACAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACGGCCGAA 
ATCCTGCGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTACATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCATCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCAGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGTC TGAGGATAGC CAGACCCCCC AAACAAGGCT TGAATAACTC TCCACCCGTG 
AAAGAATTCA AGGAGAGCTC TGCAGCCGAG GCCTTCCGGT GCCGCAGCAT CAGTGTGTCT 
GAACATGTGG TCCGCAGCAG GATACAGACA TCCCTCACCA GTGCCAGCCT GGGGTCTGCA 
GACGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA GCCTCCATCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCCCGATAC GTCTTCTCCA ACTTCACAGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTGGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGTGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGCAT GAATGTGAGA 
CAGACGAAGG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGTTC TATGTCAGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAT 
GTGCCGGCCT CCCAGTTCCC GGGCAGTGCC GCTTCTCCAG GACCACAGAC TGCACCAGCC 
GCGAAGCCTG AGAAGGCCTC GGCCGGCGCC CGGCTTCCTG TGCAGGAGAA GACGAACCTG 
GCCGCCTACG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CAGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCGCTG TCGGTGCCAG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCCG CCGTGGTCAT GGAGGAGGGA 
AGTCCTGGCG AGGTTCCTGT GCCAGTGGAG CCCCCAGGAT TGGAGGACTT TGAGGCGGCG 
CTAGGCGTGG ACAGGCGCAC AGACGCCTAC AGCAGGTCGT CCTCAACCTC CAGCCAGGAG 
GAGAAGTCAT TCCACGCAGA GGAGCTGGTT GCCGGGGGAA TCCCCATTGA ACGAGCCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCACA GCCCCTGAGC 
AAGTCTAGCT CCTCTCCTGA GCTGCAGACC CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCTGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GTTCACAGTC AGGGATCCTG 
GACGGGGAAA GTGCTGCCTG GCCAGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCACCC AGTGGCCTCC GGCCCCGCGG TTACACCATC 
TCCGACTCGG CCCCATCACG CAGGGGCAGG AGGGTAGAAA GGGATGCCTT TAAGAGCAGA 
GCCACAGCTT CCAGTGCAGA GAAAGTGCCA GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CGCCTTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT GGGAGAAGGC CAGAGCAACA GTGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTCAAGGACT GCCAGCCAGA CAAGGTGTAC CTGGGCGGCC TGGACGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGACGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAT CGTTGCGACA AGAAGCGCCA CCTGGGCAAC 
GATTTCGTGT CCATTGTCTA CAATGACTCT GGCGAGGACT TCAAGCTCGG CACCATCAAG 
ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAGATCGT GTCTGACCGC AACCTGCCTT 
TTGTGGCCCG TCAGATGGCC CTGCATGCAA ACATGGCCTC ACAGGTGCAC CACAGCCGCT 
CCAACCCCAC CGACATCTAC CCCTCCAAGT GGATTGCCCG GCTCCGCCAC ATCAAGAGAC 
TCCGCCAGCG GATCTGCGAG GAAGCCGCCT ACTCCAACCC CAGCCTGCCT CTGGTGCACC 
CCCCATCCCA TACCAAAGGG CCTGCACAGA CTCCAGCCGA GCCCACGCCT GGCTACGAGA 
TGGGCCAGCG GAAGCGCCTC ATCTCCTCTG TGGAGGACTT CACCGAGTTC GTGTGAGGCT 
GGGGCCCTCC CTGCTGCGCC CGCCCTGGAC GCTATTTTGT GTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014980838.1
CDS	241..6048
Translation	MLRSGRGATCCSFSPPGRRGLLPTPPRSSPGSQAPVPRQHPGAQGVHAGLLRTTHPGGPRGPRHVTQGSPAFPLEGCLELQLPASSVAALCAAPLPEVRVALPAASGARGVRLSPRRGGPARGGAAREGCSGASWPTMAKPTSKDSGLKEKFRILFGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLSNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIIQGQGERLGVLRALFFKVIKDYPSNEDLHERLDVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCICDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKTWRALWTPAWPRSCLTATCLLWPVRWPCMQTWPHRCTTAAPTPPTSTPPSGLPGSATSRDSASGSARKPPTPTPACLWCTPHPIPKGLHRLQPSPRLATRWASGSASSPLWRTSPSSCEAGALPAAPALDAILCQ

Target ORF information:

RefSeq Version	XM_015125352.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015125352.1

ORF Insert Sequence:

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAAGCTCCGT GGCGGCCCTC TGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
GCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAACAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACGGCCGAA 
ATCCTGCGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTACATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCATCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCAGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGTC TGAGGATAGC CAGACCCCCC AAACAAGGCT TGAATAACTC TCCACCCGTG 
AAAGAATTCA AGGAGAGCTC TGCAGCCGAG GCCTTCCGGT GCCGCAGCAT CAGTGTGTCT 
GAACATGTGG TCCGCAGCAG GATACAGACA TCCCTCACCA GTGCCAGCCT GGGGTCTGCA 
GACGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA GCCTCCATCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCCCGATAC GTCTTCTCCA ACTTCACAGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTGGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGTGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGCAT GAATGTGAGA 
CAGACGAAGG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGTTC TATGTCAGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAT 
GTGCCGGCCT CCCAGTTCCC GGGCAGTGCC GCTTCTCCAG GACCACAGAC TGCACCAGCC 
GCGAAGCCTG AGAAGGCCTC GGCCGGCGCC CGGCTTCCTG TGCAGGAGAA GACGAACCTG 
GCCGCCTACG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CAGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCGCTG TCGGTGCCAG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCCG CCGTGGTCAT GGAGGAGGGA 
AGTCCTGGCG AGGTTCCTGT GCCAGTGGAG CCCCCAGGAT TGGAGGACTT TGAGGCGGCG 
CTAGGCGTGG ACAGGCGCAC AGACGCCTAC AGCAGGTCGT CCTCAACCTC CAGCCAGGAG 
GAGAAGTCAT TCCACGCAGA GGAGCTGGTT GCCGGGGGAA TCCCCATTGA ACGAGCCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCACA GCCCCTGAGC 
AAGTCTAGCT CCTCTCCTGA GCTGCAGACC CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCTGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GTTCACAGTC AGGGATCCTG 
GACGGGGAAA GTGCTGCCTG GCCAGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCACCC AGTGGCCTCC GGCCCCGCGG TTACACCATC 
TCCGACTCGG CCCCATCACG CAGGGGCAGG AGGGTAGAAA GGGATGCCTT TAAGAGCAGA 
GCCACAGCTT CCAGTGCAGA GAAAGTGCCA GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CGCCTTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT GGGAGAAGGC CAGAGCAACA GTGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTCAAGGACT GCCAGCCAGA CAAGGTGTAC CTGGGCGGCC TGGACGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGACGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAT CGTTGCGACA AGAAGCGCCA CCTGGGCAAC 
GATTTCGTGT CCATTGTCTA CAATGACTCT GGCGAGGACT TCAAGCTCGG CACCATCAAG 
ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAGATCGT GTCTGACCGC AACCTGCCTT 
TTGTGGCCCG TCAGATGGCC CTGCATGCAA ACATGGCCTC ACAGGTGCAC CACAGCCGCT 
CCAACCCCAC CGACATCTAC CCCTCCAAGT GGATTGCCCG GCTCCGCCAC ATCAAGAGAC 
TCCGCCAGCG GATCTGCGAG GAAGCCGCCT ACTCCAACCC CAGCCTGCCT CTGGTGCACC 
CCCCATCCCA TACCAAAGGG CCTGCACAGA CTCCAGCCGA GCCCACGCCT GGCTACGAGA 
TGGGCCAGCG GAAGCGCCTC ATCTCCTCTG TGGAGGACTT CACCGAGTTC GTGTGAGGCT 
GGGGCCCTCC CTGCTGCGCC CGCCCTGGAC GCTATTTTGT GTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_001083508.3 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_001083508.2 Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA.

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CloneID	OMb54733
Clone ID Related Accession (Same CDS sequence)	XM_001083508.3
Accession Version	XM_001083508.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805823.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 21, 2015 this sequence version replaced XM_001083508.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAAGCTCCGT GGCGGCCCTC TGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
GCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAACAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACGGCCGAA 
ATCCTGCGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTACATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCATCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCAGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGTC TGAGGATAGC CAGACCCCCC AAACAAGGCT TGAATAACTC TCCACCCGTG 
AAAGAATTCA AGGAGAGCTC TGCAGCCGAG GCCTTCCGGT GCCGCAGCAT CAGTGTGTCT 
GAACATGTGG TCCGCAGCAG GATACAGACA TCCCTCACCA GTGCCAGCCT GGGGTCTGCA 
GACGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA GCCTCCATCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCCCGATAC GTCTTCTCCA ACTTCACAGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTGGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGTGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGCAT GAATGTGAGA 
CAGACGAAGG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGTTC TATGTCAGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAT 
GTGCCGGCCT CCCAGTTCCC GGGCAGTGCC GCTTCTCCAG GACCACAGAC TGCACCAGCC 
GCGAAGCCTG AGAAGGCCTC GGCCGGCGCC CGGCTTCCTG TGCAGGAGAA GACGAACCTG 
GCCGCCTACG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CAGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCGCTG TCGGTGCCAG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGACTCC GCCGTGGTCA TGGAGGAGGG AAGTCCTGGC 
GAGGTTCCTG TGCCAGTGGA GCCCCCAGGA TTGGAGGACT TTGAGGCGGC GCTAGGCGTG 
GACAGGCGCA CAGACGCCTA CAGCAGGTCG TCCTCAACCT CCAGCCAGGA GGAGAAGTCA 
TTCCACGCAG AGGAGCTGGT TGCCGGGGGA ATCCCCATTG AACGAGCCGT CTCCTCGGAG 
GGTGGCCGGC CCTCTGTGGA CCTCTCCTTC CAGCCCTCAC AGCCCCTGAG CAAGTCTAGC 
TCCTCTCCTG AGCTGCAGAC CCTGCAGGAC ATCCTCGGGG ACCCTGGGGA CAAGGCTGAC 
GTGGGCCGGC TGAGCCCTGA GGTTAAGGCC CGTTCACAGT CAGGGATCCT GGACGGGGAA 
AGTGCTGCCT GGCCAGCCTC GGGCGAAGAC AGTCGGGGCC AGCCCGAGGG TCCCTTGCCT 
TCCAGCTCCC CCCGCTCACC CAGTGGCCTC CGGCCCCGCG GTTACACCAT CTCCGACTCG 
GCCCCATCAC GCAGGGGCAG GAGGGTAGAA AGGGATGCCT TTAAGAGCAG AGCCACAGCT 
TCCAGTGCAG AGAAAGTGCC AGGCATCAAC CCCAGTTTCG TGTTCCTGCA GCTCTACCAT 
TCGCCTTTCT TTGGCGACGA GTCAAACAAG CCAATCCTGC TGCCCAATGA GTCACAGTCC 
TTTGAGCGGT CGGTGCAGCT CCTCGACCAG ATCCCATCAT ACGACACCCA CAAGATCGCC 
GTCCTGTATG TGGGAGAAGG CCAGAGCAAC AGTGAGCTCG CCATCCTGTC CAATGAGCAT 
GGCTCCTACA GGTACACGGA GTTCCTGACG GGCCTGGGCC GGCTCATCGA GCTCAAGGAC 
TGCCAGCCAG ACAAGGTGTA CCTGGGCGGC CTGGACGTGT GTGGTGAGGA CGGCCAGTTC 
ACCTACTGCT GGCACGACGA CATCATGCAA GCCGTCTTCC ACATCGCCAC CCTGATGCCC 
ACCAAGGACG TGGACAAGCA TCGTTGCGAC AAGAAGCGCC ACCTGGGCAA CGATTTCGTG 
TCCATTGTCT ACAATGACTC TGGCGAGGAC TTCAAGCTCG GCACCATCAA GGGCCAGTTC 
AACTTTGTCC ACGTGATCAT CACCCCGCTG GATTATGAGT GCAACCTGGT GTCCCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAGATCGT GTCTGACCGC 
AACCTGCCTT TTGTGGCCCG TCAGATGGCC CTGCATGCAA ACATGGCCTC ACAGGTGCAC 
CACAGCCGCT CCAACCCCAC CGACATCTAC CCCTCCAAGT GGATTGCCCG GCTCCGCCAC 
ATCAAGAGAC TCCGCCAGCG GATCTGCGAG GAAGCCGCCT ACTCCAACCC CAGCCTGCCT 
CTGGTGCACC CCCCATCCCA TACCAAAGGG CCTGCACAGA CTCCAGCCGA GCCCACGCCT 
GGCTACGAGA TGGGCCAGCG GAAGCGCCTC ATCTCCTCTG TGGAGGACTT CACCGAGTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001083508.2
CDS	237..6002
Translation	MLRSGRGATCCSFSPPGRRGLLPTPPRSSPGSQAPVPRQHPGAQGVHAGLLRTTHPGGPRGPRHVTQGSPAFPLEGCLELQLPASSVAALCAAPLPEVRVALPAASGARGVRLSPRRGGPARGGAAREGCSGASWPTMAKPTSKDSGLKEKFRILFGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLSNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIIQGQGERLGVLRALFFKVIKDYPSNEDLHERLDVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCICDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTDSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKGPAQTPAEPTPGYEMGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_001083508.3
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001083508.3

ORF Insert Sequence:

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAAGCTCCGT GGCGGCCCTC TGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
GCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAACAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACGGCCGAA 
ATCCTGCGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTACATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCATCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCAGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGTC TGAGGATAGC CAGACCCCCC AAACAAGGCT TGAATAACTC TCCACCCGTG 
AAAGAATTCA AGGAGAGCTC TGCAGCCGAG GCCTTCCGGT GCCGCAGCAT CAGTGTGTCT 
GAACATGTGG TCCGCAGCAG GATACAGACA TCCCTCACCA GTGCCAGCCT GGGGTCTGCA 
GACGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA GCCTCCATCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCCCGATAC GTCTTCTCCA ACTTCACAGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTGGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGTGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGCAT GAATGTGAGA 
CAGACGAAGG AGGCGCCAGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGTTC TATGTCAGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAT 
GTGCCGGCCT CCCAGTTCCC GGGCAGTGCC GCTTCTCCAG GACCACAGAC TGCACCAGCC 
GCGAAGCCTG AGAAGGCCTC GGCCGGCGCC CGGCTTCCTG TGCAGGAGAA GACGAACCTG 
GCCGCCTACG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CAGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCGCTG TCGGTGCCAG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGACTCC GCCGTGGTCA TGGAGGAGGG AAGTCCTGGC 
GAGGTTCCTG TGCCAGTGGA GCCCCCAGGA TTGGAGGACT TTGAGGCGGC GCTAGGCGTG 
GACAGGCGCA CAGACGCCTA CAGCAGGTCG TCCTCAACCT CCAGCCAGGA GGAGAAGTCA 
TTCCACGCAG AGGAGCTGGT TGCCGGGGGA ATCCCCATTG AACGAGCCGT CTCCTCGGAG 
GGTGGCCGGC CCTCTGTGGA CCTCTCCTTC CAGCCCTCAC AGCCCCTGAG CAAGTCTAGC 
TCCTCTCCTG AGCTGCAGAC CCTGCAGGAC ATCCTCGGGG ACCCTGGGGA CAAGGCTGAC 
GTGGGCCGGC TGAGCCCTGA GGTTAAGGCC CGTTCACAGT CAGGGATCCT GGACGGGGAA 
AGTGCTGCCT GGCCAGCCTC GGGCGAAGAC AGTCGGGGCC AGCCCGAGGG TCCCTTGCCT 
TCCAGCTCCC CCCGCTCACC CAGTGGCCTC CGGCCCCGCG GTTACACCAT CTCCGACTCG 
GCCCCATCAC GCAGGGGCAG GAGGGTAGAA AGGGATGCCT TTAAGAGCAG AGCCACAGCT 
TCCAGTGCAG AGAAAGTGCC AGGCATCAAC CCCAGTTTCG TGTTCCTGCA GCTCTACCAT 
TCGCCTTTCT TTGGCGACGA GTCAAACAAG CCAATCCTGC TGCCCAATGA GTCACAGTCC 
TTTGAGCGGT CGGTGCAGCT CCTCGACCAG ATCCCATCAT ACGACACCCA CAAGATCGCC 
GTCCTGTATG TGGGAGAAGG CCAGAGCAAC AGTGAGCTCG CCATCCTGTC CAATGAGCAT 
GGCTCCTACA GGTACACGGA GTTCCTGACG GGCCTGGGCC GGCTCATCGA GCTCAAGGAC 
TGCCAGCCAG ACAAGGTGTA CCTGGGCGGC CTGGACGTGT GTGGTGAGGA CGGCCAGTTC 
ACCTACTGCT GGCACGACGA CATCATGCAA GCCGTCTTCC ACATCGCCAC CCTGATGCCC 
ACCAAGGACG TGGACAAGCA TCGTTGCGAC AAGAAGCGCC ACCTGGGCAA CGATTTCGTG 
TCCATTGTCT ACAATGACTC TGGCGAGGAC TTCAAGCTCG GCACCATCAA GGGCCAGTTC 
AACTTTGTCC ACGTGATCAT CACCCCGCTG GATTATGAGT GCAACCTGGT GTCCCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAGATCGT GTCTGACCGC 
AACCTGCCTT TTGTGGCCCG TCAGATGGCC CTGCATGCAA ACATGGCCTC ACAGGTGCAC 
CACAGCCGCT CCAACCCCAC CGACATCTAC CCCTCCAAGT GGATTGCCCG GCTCCGCCAC 
ATCAAGAGAC TCCGCCAGCG GATCTGCGAG GAAGCCGCCT ACTCCAACCC CAGCCTGCCT 
CTGGTGCACC CCCCATCCCA TACCAAAGGG CCTGCACAGA CTCCAGCCGA GCCCACGCCT 
GGCTACGAGA TGGGCCAGCG GAAGCGCCTC ATCTCCTCTG TGGAGGACTT CACCGAGTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015125353.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014980839.1 Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA.

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CloneID	OMb54734
Clone ID Related Accession (Same CDS sequence)	XM_015125353.1
Accession Version	XM_015125353.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805823.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAAGCTCCGT GGCGGCCCTC TGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
GCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAACAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACGGCCGAA 
ATCCTGCGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTACATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCATCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCAGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGCA GGATACAGAC ATCCCTCACC AGTGCCAGCC TGGGGTCTGC AGACGAGAAC 
TCCGTGGCCC AGGCTGACGA TAGCCTGAAA AGCCTCCATC TGGAGCTCAC GGAAACCTGT 
CTGGACATGA TGGCCCGATA CGTCTTCTCC AACTTCACAG CTGTCCCGAA GAGGTCTCCT 
GTGGGCGAGT TCCTCCTGGC GGGTGGCAGG ACCAAAACCT GGCTGGTTGG GAACAAGCTT 
GTCACTGTGA CGACGAGTGT GGGAACCGGG ACCCGGTCGT TACTAGGCCT GGACTCGGGG 
GAGCTGCAGT CCGGCCCGGA GTCGAGCTCC AGCCCCGGCA TGAATGTGAG ACAGACGAAG 
GAGGCGCCAG CCAAGCTGGA GTCCCAGGCT GGGCAGCAGG TGTCCCGTGG GGCCCGGGAC 
CGGGTCCGTT CTATGTCAGG GGGCCATGGT CTTCGAGTTG GCGCCCTGGA TGTGCCGGCC 
TCCCAGTTCC CGGGCAGTGC CGCTTCTCCA GGACCACAGA CTGCACCAGC CGCGAAGCCT 
GAGAAGGCCT CGGCCGGCGC CCGGCTTCCT GTGCAGGAGA AGACGAACCT GGCCGCCTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCGGAG ATCCTGGTCC GGAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCTTTCT CCTCGGACAT CAACAACATG 
CCCCTGCAGG AGCTGTCCAA CGCCCTCATG GCAGCTGAGC GCTTCAAGGA GCACCGGGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCA GCAGCCAGCA CGGCCAAACC CCCTCCTCTG 
CCTCGCTCCA ACACAGTGGC CTCTTTCTCC TCCCTGTACC AGTCCAGCTG CCAAGGACAG 
CTGCACAGGA GCGTTTCCTG GGCAGACTCC GCCGTGGTCA TGGAGGAGGG AAGTCCTGGC 
GAGGTTCCTG TGCCAGTGGA GCCCCCAGGA TTGGAGGACT TTGAGGCGGC GCTAGGCGTG 
GACAGGCGCA CAGACGCCTA CAGCAGGTCG TCCTCAACCT CCAGCCAGGA GGAGAAGTCA 
TTCCACGCAG AGGAGCTGGT TGCCGGGGGA ATCCCCATTG AACGAGCCGT CTCCTCGGAG 
GGTGGCCGGC CCTCTGTGGA CCTCTCCTTC CAGCCCTCAC AGCCCCTGAG CAAGTCTAGC 
TCCTCTCCTG AGCTGCAGAC CCTGCAGGAC ATCCTCGGGG ACCCTGGGGA CAAGGCTGAC 
GTGGGCCGGC TGAGCCCTGA GGTTAAGGCC CGTTCACAGT CAGGGATCCT GGACGGGGAA 
AGTGCTGCCT GGCCAGCCTC GGGCGAAGAC AGTCGGGGCC AGCCCGAGGG TCCCTTGCCT 
TCCAGCTCCC CCCGCTCACC CAGTGGCCTC CGGCCCCGCG GTTACACCAT CTCCGACTCG 
GCCCCATCAC GCAGGGGCAG GAGGGTAGAA AGGGATGCCT TTAAGAGCAG AGCCACAGCT 
TCCAGTGCAG AGAAAGTGCC AGGCATCAAC CCCAGTTTCG TGTTCCTGCA GCTCTACCAT 
TCGCCTTTCT TTGGCGACGA GTCAAACAAG CCAATCCTGC TGCCCAATGA GTCACAGTCC 
TTTGAGCGGT CGGTGCAGCT CCTCGACCAG ATCCCATCAT ACGACACCCA CAAGATCGCC 
GTCCTGTATG TGGGAGAAGG CCAGAGCAAC AGTGAGCTCG CCATCCTGTC CAATGAGCAT 
GGCTCCTACA GGTACACGGA GTTCCTGACG GGCCTGGGCC GGCTCATCGA GCTCAAGGAC 
TGCCAGCCAG ACAAGGTGTA CCTGGGCGGC CTGGACGTGT GTGGTGAGGA CGGCCAGTTC 
ACCTACTGCT GGCACGACGA CATCATGCAA GCCGTCTTCC ACATCGCCAC CCTGATGCCC 
ACCAAGGACG TGGACAAGCA TCGTTGCGAC AAGAAGCGCC ACCTGGGCAA CGATTTCGTG 
TCCATTGTCT ACAATGACTC TGGCGAGGAC TTCAAGCTCG GCACCATCAA GGGCCAGTTC 
AACTTTGTCC ACGTGATCAT CACCCCGCTG GATTATGAGT GCAACCTGGT GTCCCTGCAG 
TGCAGGAAAG GTGGGGCCAG GGTGGGGCCG GTGTCCCTGC AGTGCAGGAA AGACATGGAG 
GGCCTTGTGG ACACCAGCGT GGCCAAGATC GTGTCTGACC GCAACCTGCC TTTTGTGGCC 
CGTCAGATGG CCCTGCATGC AAACATGGCC TCACAGGTGC ACCACAGCCG CTCCAACCCC 
ACCGACATCT ACCCCTCCAA GTGGATTGCC CGGCTCCGCC ACATCAAGAG ACTCCGCCAG 
CGGATCTGCG AGGAAGCCGC CTACTCCAAC CCCAGCCTGC CTCTGGTGCA CCCCCCATCC 
CATACCAAAG GGCCTGCACA GACTCCAGCC GAGCCCACGC CTGGCTACGA GATGGGCCAG 
CGGAAGCGCC TCATCTCCTC TGTGGAGGAC TTCACCGAGT TCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014980839.1
CDS	238..5985
Translation	MLRSGRGATCCSFSPPGRRGLLPTPPRSSPGSQAPVPRQHPGAQGVHAGLLRTTHPGGPRGPRHVTQGSPAFPLEGCLELQLPASSVAALCAAPLPEVRVALPAASGARGVRLSPRRGGPARGGAAREGCSGASWPTMAKPTSKDSGLKEKFRILFGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLSNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIIQGQGERLGVLRALFFKVIKDYPSNEDLHERLDVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCICDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKGGARVGPVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKGPAQTPAEPTPGYEMGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_015125353.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015125353.1

ORF Insert Sequence:

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAAGCTCCGT GGCGGCCCTC TGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
GCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAACAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACGGCCGAA 
ATCCTGCGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTACATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCATCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCAGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGCA GGATACAGAC ATCCCTCACC AGTGCCAGCC TGGGGTCTGC AGACGAGAAC 
TCCGTGGCCC AGGCTGACGA TAGCCTGAAA AGCCTCCATC TGGAGCTCAC GGAAACCTGT 
CTGGACATGA TGGCCCGATA CGTCTTCTCC AACTTCACAG CTGTCCCGAA GAGGTCTCCT 
GTGGGCGAGT TCCTCCTGGC GGGTGGCAGG ACCAAAACCT GGCTGGTTGG GAACAAGCTT 
GTCACTGTGA CGACGAGTGT GGGAACCGGG ACCCGGTCGT TACTAGGCCT GGACTCGGGG 
GAGCTGCAGT CCGGCCCGGA GTCGAGCTCC AGCCCCGGCA TGAATGTGAG ACAGACGAAG 
GAGGCGCCAG CCAAGCTGGA GTCCCAGGCT GGGCAGCAGG TGTCCCGTGG GGCCCGGGAC 
CGGGTCCGTT CTATGTCAGG GGGCCATGGT CTTCGAGTTG GCGCCCTGGA TGTGCCGGCC 
TCCCAGTTCC CGGGCAGTGC CGCTTCTCCA GGACCACAGA CTGCACCAGC CGCGAAGCCT 
GAGAAGGCCT CGGCCGGCGC CCGGCTTCCT GTGCAGGAGA AGACGAACCT GGCCGCCTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCGGAG ATCCTGGTCC GGAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCTTTCT CCTCGGACAT CAACAACATG 
CCCCTGCAGG AGCTGTCCAA CGCCCTCATG GCAGCTGAGC GCTTCAAGGA GCACCGGGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCA GCAGCCAGCA CGGCCAAACC CCCTCCTCTG 
CCTCGCTCCA ACACAGTGGC CTCTTTCTCC TCCCTGTACC AGTCCAGCTG CCAAGGACAG 
CTGCACAGGA GCGTTTCCTG GGCAGACTCC GCCGTGGTCA TGGAGGAGGG AAGTCCTGGC 
GAGGTTCCTG TGCCAGTGGA GCCCCCAGGA TTGGAGGACT TTGAGGCGGC GCTAGGCGTG 
GACAGGCGCA CAGACGCCTA CAGCAGGTCG TCCTCAACCT CCAGCCAGGA GGAGAAGTCA 
TTCCACGCAG AGGAGCTGGT TGCCGGGGGA ATCCCCATTG AACGAGCCGT CTCCTCGGAG 
GGTGGCCGGC CCTCTGTGGA CCTCTCCTTC CAGCCCTCAC AGCCCCTGAG CAAGTCTAGC 
TCCTCTCCTG AGCTGCAGAC CCTGCAGGAC ATCCTCGGGG ACCCTGGGGA CAAGGCTGAC 
GTGGGCCGGC TGAGCCCTGA GGTTAAGGCC CGTTCACAGT CAGGGATCCT GGACGGGGAA 
AGTGCTGCCT GGCCAGCCTC GGGCGAAGAC AGTCGGGGCC AGCCCGAGGG TCCCTTGCCT 
TCCAGCTCCC CCCGCTCACC CAGTGGCCTC CGGCCCCGCG GTTACACCAT CTCCGACTCG 
GCCCCATCAC GCAGGGGCAG GAGGGTAGAA AGGGATGCCT TTAAGAGCAG AGCCACAGCT 
TCCAGTGCAG AGAAAGTGCC AGGCATCAAC CCCAGTTTCG TGTTCCTGCA GCTCTACCAT 
TCGCCTTTCT TTGGCGACGA GTCAAACAAG CCAATCCTGC TGCCCAATGA GTCACAGTCC 
TTTGAGCGGT CGGTGCAGCT CCTCGACCAG ATCCCATCAT ACGACACCCA CAAGATCGCC 
GTCCTGTATG TGGGAGAAGG CCAGAGCAAC AGTGAGCTCG CCATCCTGTC CAATGAGCAT 
GGCTCCTACA GGTACACGGA GTTCCTGACG GGCCTGGGCC GGCTCATCGA GCTCAAGGAC 
TGCCAGCCAG ACAAGGTGTA CCTGGGCGGC CTGGACGTGT GTGGTGAGGA CGGCCAGTTC 
ACCTACTGCT GGCACGACGA CATCATGCAA GCCGTCTTCC ACATCGCCAC CCTGATGCCC 
ACCAAGGACG TGGACAAGCA TCGTTGCGAC AAGAAGCGCC ACCTGGGCAA CGATTTCGTG 
TCCATTGTCT ACAATGACTC TGGCGAGGAC TTCAAGCTCG GCACCATCAA GGGCCAGTTC 
AACTTTGTCC ACGTGATCAT CACCCCGCTG GATTATGAGT GCAACCTGGT GTCCCTGCAG 
TGCAGGAAAG GTGGGGCCAG GGTGGGGCCG GTGTCCCTGC AGTGCAGGAA AGACATGGAG 
GGCCTTGTGG ACACCAGCGT GGCCAAGATC GTGTCTGACC GCAACCTGCC TTTTGTGGCC 
CGTCAGATGG CCCTGCATGC AAACATGGCC TCACAGGTGC ACCACAGCCG CTCCAACCCC 
ACCGACATCT ACCCCTCCAA GTGGATTGCC CGGCTCCGCC ACATCAAGAG ACTCCGCCAG 
CGGATCTGCG AGGAAGCCGC CTACTCCAAC CCCAGCCTGC CTCTGGTGCA CCCCCCATCC 
CATACCAAAG GGCCTGCACA GACTCCAGCC GAGCCCACGC CTGGCTACGA GATGGGCCAG 
CGGAAGCGCC TCATCTCCTC TGTGGAGGAC TTCACCGAGT TCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015125354.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014980840.1 Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X8, mRNA.

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CloneID	OMb54735
Clone ID Related Accession (Same CDS sequence)	XM_015125354.1
Accession Version	XM_015125354.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805823.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAAGCTCCGT GGCGGCCCTC TGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
GCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAACAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACGGCCGAA 
ATCCTGCGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTACATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCATCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCAGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGGA TACAGACATC CCTCACCAGT GCCAGCCTGG GGTCTGCAGA CGAGAACTCC 
GTGGCCCAGG CTGACGATAG CCTGAAAAGC CTCCATCTGG AGCTCACGGA AACCTGTCTG 
GACATGATGG CCCGATACGT CTTCTCCAAC TTCACAGCTG TCCCGAAGAG GTCTCCTGTG 
GGCGAGTTCC TCCTGGCGGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACTGTGACGA CGAGTGTGGG AACCGGGACC CGGTCGTTAC TAGGCCTGGA CTCGGGGGAG 
CTGCAGTCCG GCCCGGAGTC GAGCTCCAGC CCCGGCATGA ATGTGAGACA GACGAAGGAG 
GCGCCAGCCA AGCTGGAGTC CCAGGCTGGG CAGCAGGTGT CCCGTGGGGC CCGGGACCGG 
GTCCGTTCTA TGTCAGGGGG CCATGGTCTT CGAGTTGGCG CCCTGGATGT GCCGGCCTCC 
CAGTTCCCGG GCAGTGCCGC TTCTCCAGGA CCACAGACTG CACCAGCCGC GAAGCCTGAG 
AAGGCCTCGG CCGGCGCCCG GCTTCCTGTG CAGGAGAAGA CGAACCTGGC CGCCTACGTG 
CCCCTGCTGA CCCAGGGCTG GGCGGAGATC CTGGTCCGGA GGCCCACAGG GAACACCAGC 
TGGCTGATGA GCCTGGAGAA CCCGCTCAGC CCTTTCTCCT CGGACATCAA CAACATGCCC 
CTGCAGGAGC TGTCCAACGC CCTCATGGCA GCTGAGCGCT TCAAGGAGCA CCGGGACACA 
GCCCTGTACA AGTCGCTGTC GGTGCCAGCA GCCAGCACGG CCAAACCCCC TCCTCTGCCT 
CGCTCCAACA CAGTGGCCTC TTTCTCCTCC CTGTACCAGT CCAGCTGCCA AGGACAGCTG 
CACAGGAGCG TTTCCTGGGC AGACTCCGCC GTGGTCATGG AGGAGGGAAG TCCTGGCGAG 
GTTCCTGTGC CAGTGGAGCC CCCAGGATTG GAGGACTTTG AGGCGGCGCT AGGCGTGGAC 
AGGCGCACAG ACGCCTACAG CAGGTCGTCC TCAACCTCCA GCCAGGAGGA GAAGTCATTC 
CACGCAGAGG AGCTGGTTGC CGGGGGAATC CCCATTGAAC GAGCCGTCTC CTCGGAGGGT 
GGCCGGCCCT CTGTGGACCT CTCCTTCCAG CCCTCACAGC CCCTGAGCAA GTCTAGCTCC 
TCTCCTGAGC TGCAGACCCT GCAGGACATC CTCGGGGACC CTGGGGACAA GGCTGACGTG 
GGCCGGCTGA GCCCTGAGGT TAAGGCCCGT TCACAGTCAG GGATCCTGGA CGGGGAAAGT 
GCTGCCTGGC CAGCCTCGGG CGAAGACAGT CGGGGCCAGC CCGAGGGTCC CTTGCCTTCC 
AGCTCCCCCC GCTCACCCAG TGGCCTCCGG CCCCGCGGTT ACACCATCTC CGACTCGGCC 
CCATCACGCA GGGGCAGGAG GGTAGAAAGG GATGCCTTTA AGAGCAGAGC CACAGCTTCC 
AGTGCAGAGA AAGTGCCAGG CATCAACCCC AGTTTCGTGT TCCTGCAGCT CTACCATTCG 
CCTTTCTTTG GCGACGAGTC AAACAAGCCA ATCCTGCTGC CCAATGAGTC ACAGTCCTTT 
GAGCGGTCGG TGCAGCTCCT CGACCAGATC CCATCATACG ACACCCACAA GATCGCCGTC 
CTGTATGTGG GAGAAGGCCA GAGCAACAGT GAGCTCGCCA TCCTGTCCAA TGAGCATGGC 
TCCTACAGGT ACACGGAGTT CCTGACGGGC CTGGGCCGGC TCATCGAGCT CAAGGACTGC 
CAGCCAGACA AGGTGTACCT GGGCGGCCTG GACGTGTGTG GTGAGGACGG CCAGTTCACC 
TACTGCTGGC ACGACGACAT CATGCAAGCC GTCTTCCACA TCGCCACCCT GATGCCCACC 
AAGGACGTGG ACAAGCATCG TTGCGACAAG AAGCGCCACC TGGGCAACGA TTTCGTGTCC 
ATTGTCTACA ATGACTCTGG CGAGGACTTC AAGCTCGGCA CCATCAAGGG CCAGTTCAAC 
TTTGTCCACG TGATCATCAC CCCGCTGGAT TATGAGTGCA ACCTGGTGTC CCTGCAGTGC 
AGGAAAGGTG GGGCCAGGGT GGGGCCGGTG TCCCTGCAGT GCAGGAAAGA CATGGAGGGC 
CTTGTGGACA CCAGCGTGGC CAAGATCGTG TCTGACCGCA ACCTGCCTTT TGTGGCCCGT 
CAGATGGCCC TGCATGCAAA CATGGCCTCA CAGGTGCACC ACAGCCGCTC CAACCCCACC 
GACATCTACC CCTCCAAGTG GATTGCCCGG CTCCGCCACA TCAAGAGACT CCGCCAGCGG 
ATCTGCGAGG AAGCCGCCTA CTCCAACCCC AGCCTGCCTC TGGTGCACCC CCCATCCCAT 
ACCAAAGGGC CTGCACAGAC TCCAGCCGAG CCCACGCCTG GCTACGAGAT GGGCCAGCGG 
AAGCGCCTCA TCTCCTCTGT GGAGGACTTC ACCGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014980840.1
CDS	238..5982
Translation	MLRSGRGATCCSFSPPGRRGLLPTPPRSSPGSQAPVPRQHPGAQGVHAGLLRTTHPGGPRGPRHVTQGSPAFPLEGCLELQLPASSVAALCAAPLPEVRVALPAASGARGVRLSPRRGGPARGGAAREGCSGASWPTMAKPTSKDSGLKEKFRILFGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLSNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIIQGQGERLGVLRALFFKVIKDYPSNEDLHERLDVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCICDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKGGARVGPVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKGPAQTPAEPTPGYEMGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_015125354.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015125354.1

ORF Insert Sequence:

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAAGCTCCGT GGCGGCCCTC TGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
GCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAACAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACGGCCGAA 
ATCCTGCGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTACATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCATCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCAGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGGA TACAGACATC CCTCACCAGT GCCAGCCTGG GGTCTGCAGA CGAGAACTCC 
GTGGCCCAGG CTGACGATAG CCTGAAAAGC CTCCATCTGG AGCTCACGGA AACCTGTCTG 
GACATGATGG CCCGATACGT CTTCTCCAAC TTCACAGCTG TCCCGAAGAG GTCTCCTGTG 
GGCGAGTTCC TCCTGGCGGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACTGTGACGA CGAGTGTGGG AACCGGGACC CGGTCGTTAC TAGGCCTGGA CTCGGGGGAG 
CTGCAGTCCG GCCCGGAGTC GAGCTCCAGC CCCGGCATGA ATGTGAGACA GACGAAGGAG 
GCGCCAGCCA AGCTGGAGTC CCAGGCTGGG CAGCAGGTGT CCCGTGGGGC CCGGGACCGG 
GTCCGTTCTA TGTCAGGGGG CCATGGTCTT CGAGTTGGCG CCCTGGATGT GCCGGCCTCC 
CAGTTCCCGG GCAGTGCCGC TTCTCCAGGA CCACAGACTG CACCAGCCGC GAAGCCTGAG 
AAGGCCTCGG CCGGCGCCCG GCTTCCTGTG CAGGAGAAGA CGAACCTGGC CGCCTACGTG 
CCCCTGCTGA CCCAGGGCTG GGCGGAGATC CTGGTCCGGA GGCCCACAGG GAACACCAGC 
TGGCTGATGA GCCTGGAGAA CCCGCTCAGC CCTTTCTCCT CGGACATCAA CAACATGCCC 
CTGCAGGAGC TGTCCAACGC CCTCATGGCA GCTGAGCGCT TCAAGGAGCA CCGGGACACA 
GCCCTGTACA AGTCGCTGTC GGTGCCAGCA GCCAGCACGG CCAAACCCCC TCCTCTGCCT 
CGCTCCAACA CAGTGGCCTC TTTCTCCTCC CTGTACCAGT CCAGCTGCCA AGGACAGCTG 
CACAGGAGCG TTTCCTGGGC AGACTCCGCC GTGGTCATGG AGGAGGGAAG TCCTGGCGAG 
GTTCCTGTGC CAGTGGAGCC CCCAGGATTG GAGGACTTTG AGGCGGCGCT AGGCGTGGAC 
AGGCGCACAG ACGCCTACAG CAGGTCGTCC TCAACCTCCA GCCAGGAGGA GAAGTCATTC 
CACGCAGAGG AGCTGGTTGC CGGGGGAATC CCCATTGAAC GAGCCGTCTC CTCGGAGGGT 
GGCCGGCCCT CTGTGGACCT CTCCTTCCAG CCCTCACAGC CCCTGAGCAA GTCTAGCTCC 
TCTCCTGAGC TGCAGACCCT GCAGGACATC CTCGGGGACC CTGGGGACAA GGCTGACGTG 
GGCCGGCTGA GCCCTGAGGT TAAGGCCCGT TCACAGTCAG GGATCCTGGA CGGGGAAAGT 
GCTGCCTGGC CAGCCTCGGG CGAAGACAGT CGGGGCCAGC CCGAGGGTCC CTTGCCTTCC 
AGCTCCCCCC GCTCACCCAG TGGCCTCCGG CCCCGCGGTT ACACCATCTC CGACTCGGCC 
CCATCACGCA GGGGCAGGAG GGTAGAAAGG GATGCCTTTA AGAGCAGAGC CACAGCTTCC 
AGTGCAGAGA AAGTGCCAGG CATCAACCCC AGTTTCGTGT TCCTGCAGCT CTACCATTCG 
CCTTTCTTTG GCGACGAGTC AAACAAGCCA ATCCTGCTGC CCAATGAGTC ACAGTCCTTT 
GAGCGGTCGG TGCAGCTCCT CGACCAGATC CCATCATACG ACACCCACAA GATCGCCGTC 
CTGTATGTGG GAGAAGGCCA GAGCAACAGT GAGCTCGCCA TCCTGTCCAA TGAGCATGGC 
TCCTACAGGT ACACGGAGTT CCTGACGGGC CTGGGCCGGC TCATCGAGCT CAAGGACTGC 
CAGCCAGACA AGGTGTACCT GGGCGGCCTG GACGTGTGTG GTGAGGACGG CCAGTTCACC 
TACTGCTGGC ACGACGACAT CATGCAAGCC GTCTTCCACA TCGCCACCCT GATGCCCACC 
AAGGACGTGG ACAAGCATCG TTGCGACAAG AAGCGCCACC TGGGCAACGA TTTCGTGTCC 
ATTGTCTACA ATGACTCTGG CGAGGACTTC AAGCTCGGCA CCATCAAGGG CCAGTTCAAC 
TTTGTCCACG TGATCATCAC CCCGCTGGAT TATGAGTGCA ACCTGGTGTC CCTGCAGTGC 
AGGAAAGGTG GGGCCAGGGT GGGGCCGGTG TCCCTGCAGT GCAGGAAAGA CATGGAGGGC 
CTTGTGGACA CCAGCGTGGC CAAGATCGTG TCTGACCGCA ACCTGCCTTT TGTGGCCCGT 
CAGATGGCCC TGCATGCAAA CATGGCCTCA CAGGTGCACC ACAGCCGCTC CAACCCCACC 
GACATCTACC CCTCCAAGTG GATTGCCCGG CTCCGCCACA TCAAGAGACT CCGCCAGCGG 
ATCTGCGAGG AAGCCGCCTA CTCCAACCCC AGCCTGCCTC TGGTGCACCC CCCATCCCAT 
ACCAAAGGGC CTGCACAGAC TCCAGCCGAG CCCACGCCTG GCTACGAGAT GGGCCAGCGG 
AAGCGCCTCA TCTCCTCTGT GGAGGACTTC ACCGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015125355.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014980841.1 Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X9, mRNA.

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CloneID	OMb54736
Clone ID Related Accession (Same CDS sequence)	XM_015125355.1
Accession Version	XM_015125355.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805823.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAAGCTCCGT GGCGGCCCTC TGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
GCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAACAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACGGCCGAA 
ATCCTGCGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTACATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCATCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCAGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGCA GGATACAGAC ATCCCTCACC AGTGCCAGCC TGGGGTCTGC AGACGAGAAC 
TCCGTGGCCC AGGCTGACGA TAGCCTGAAA AGCCTCCATC TGGAGCTCAC GGAAACCTGT 
CTGGACATGA TGGCCCGATA CGTCTTCTCC AACTTCACAG CTGTCCCGAA GAGGTCTCCT 
GTGGGCGAGT TCCTCCTGGC GGGTGGCAGG ACCAAAACCT GGCTGGTTGG GAACAAGCTT 
GTCACTGTGA CGACGAGTGT GGGAACCGGG ACCCGGTCGT TACTAGGCCT GGACTCGGGG 
GAGCTGCAGT CCGGCCCGGA GTCGAGCTCC AGCCCCGGCA TGAATGTGAG ACAGACGAAG 
GAGGCGCCAG CCAAGCTGGA GTCCCAGGCT GGGCAGCAGG TGTCCCGTGG GGCCCGGGAC 
CGGGTCCGTT CTATGTCAGG GGGCCATGGT CTTCGAGTTG GCGCCCTGGA TGTGCCGGCC 
TCCCAGTTCC CGGGCAGTGC CGCTTCTCCA GGACCACAGA CTGCACCAGC CGCGAAGCCT 
GAGAAGGCCT CGGCCGGCGC CCGGCTTCCT GTGCAGGAGA AGACGAACCT GGCCGCCTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCGGAG ATCCTGGTCC GGAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCTTTCT CCTCGGACAT CAACAACATG 
CCCCTGCAGG AGCTGTCCAA CGCCCTCATG GCAGCTGAGC GCTTCAAGGA GCACCGGGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCA GCAGCCAGCA CGGCCAAACC CCCTCCTCTG 
CCTCGCTCCA ACACAGACTC CGCCGTGGTC ATGGAGGAGG GAAGTCCTGG CGAGGTTCCT 
GTGCCAGTGG AGCCCCCAGG ATTGGAGGAC TTTGAGGCGG CGCTAGGCGT GGACAGGCGC 
ACAGACGCCT ACAGCAGGTC GTCCTCAACC TCCAGCCAGG AGGAGAAGTC ATTCCACGCA 
GAGGAGCTGG TTGCCGGGGG AATCCCCATT GAACGAGCCG TCTCCTCGGA GGGTGGCCGG 
CCCTCTGTGG ACCTCTCCTT CCAGCCCTCA CAGCCCCTGA GCAAGTCTAG CTCCTCTCCT 
GAGCTGCAGA CCCTGCAGGA CATCCTCGGG GACCCTGGGG ACAAGGCTGA CGTGGGCCGG 
CTGAGCCCTG AGGTTAAGGC CCGTTCACAG TCAGGGATCC TGGACGGGGA AAGTGCTGCC 
TGGCCAGCCT CGGGCGAAGA CAGTCGGGGC CAGCCCGAGG GTCCCTTGCC TTCCAGCTCC 
CCCCGCTCAC CCAGTGGCCT CCGGCCCCGC GGTTACACCA TCTCCGACTC GGCCCCATCA 
CGCAGGGGCA GGAGGGTAGA AAGGGATGCC TTTAAGAGCA GAGCCACAGC TTCCAGTGCA 
GAGAAAGTGC CAGGCATCAA CCCCAGTTTC GTGTTCCTGC AGCTCTACCA TTCGCCTTTC 
TTTGGCGACG AGTCAAACAA GCCAATCCTG CTGCCCAATG AGTCACAGTC CTTTGAGCGG 
TCGGTGCAGC TCCTCGACCA GATCCCATCA TACGACACCC ACAAGATCGC CGTCCTGTAT 
GTGGGAGAAG GCCAGAGCAA CAGTGAGCTC GCCATCCTGT CCAATGAGCA TGGCTCCTAC 
AGGTACACGG AGTTCCTGAC GGGCCTGGGC CGGCTCATCG AGCTCAAGGA CTGCCAGCCA 
GACAAGGTGT ACCTGGGCGG CCTGGACGTG TGTGGTGAGG ACGGCCAGTT CACCTACTGC 
TGGCACGACG ACATCATGCA AGCCGTCTTC CACATCGCCA CCCTGATGCC CACCAAGGAC 
GTGGACAAGC ATCGTTGCGA CAAGAAGCGC CACCTGGGCA ACGATTTCGT GTCCATTGTC 
TACAATGACT CTGGCGAGGA CTTCAAGCTC GGCACCATCA AGGGCCAGTT CAACTTTGTC 
CACGTGATCA TCACCCCGCT GGATTATGAG TGCAACCTGG TGTCCCTGCA GTGCAGGAAA 
GGTGGGGCCA GGGTGGGGCC GGTGTCCCTG CAGTGCAGGA AAGACATGGA GGGCCTTGTG 
GACACCAGCG TGGCCAAGAT CGTGTCTGAC CGCAACCTGC CTTTTGTGGC CCGTCAGATG 
GCCCTGCATG CAAACATGGC CTCACAGGTG CACCACAGCC GCTCCAACCC CACCGACATC 
TACCCCTCCA AGTGGATTGC CCGGCTCCGC CACATCAAGA GACTCCGCCA GCGGATCTGC 
GAGGAAGCCG CCTACTCCAA CCCCAGCCTG CCTCTGGTGC ACCCCCCATC CCATACCAAA 
GGGCCTGCAC AGACTCCAGC CGAGCCCACG CCTGGCTACG AGATGGGCCA GCGGAAGCGC 
CTCATCTCCT CTGTGGAGGA CTTCACCGAG TTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014980841.1
CDS	238..5916
Translation	MLRSGRGATCCSFSPPGRRGLLPTPPRSSPGSQAPVPRQHPGAQGVHAGLLRTTHPGGPRGPRHVTQGSPAFPLEGCLELQLPASSVAALCAAPLPEVRVALPAASGARGVRLSPRRGGPARGGAAREGCSGASWPTMAKPTSKDSGLKEKFRILFGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLSNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIIQGQGERLGVLRALFFKVIKDYPSNEDLHERLDVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCICDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTDSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKGGARVGPVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKGPAQTPAEPTPGYEMGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_015125355.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015125355.1

ORF Insert Sequence:

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAAGCTCCGT GGCGGCCCTC TGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
GCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAACAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACGGCCGAA 
ATCCTGCGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTACATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCATCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCAGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGCA GGATACAGAC ATCCCTCACC AGTGCCAGCC TGGGGTCTGC AGACGAGAAC 
TCCGTGGCCC AGGCTGACGA TAGCCTGAAA AGCCTCCATC TGGAGCTCAC GGAAACCTGT 
CTGGACATGA TGGCCCGATA CGTCTTCTCC AACTTCACAG CTGTCCCGAA GAGGTCTCCT 
GTGGGCGAGT TCCTCCTGGC GGGTGGCAGG ACCAAAACCT GGCTGGTTGG GAACAAGCTT 
GTCACTGTGA CGACGAGTGT GGGAACCGGG ACCCGGTCGT TACTAGGCCT GGACTCGGGG 
GAGCTGCAGT CCGGCCCGGA GTCGAGCTCC AGCCCCGGCA TGAATGTGAG ACAGACGAAG 
GAGGCGCCAG CCAAGCTGGA GTCCCAGGCT GGGCAGCAGG TGTCCCGTGG GGCCCGGGAC 
CGGGTCCGTT CTATGTCAGG GGGCCATGGT CTTCGAGTTG GCGCCCTGGA TGTGCCGGCC 
TCCCAGTTCC CGGGCAGTGC CGCTTCTCCA GGACCACAGA CTGCACCAGC CGCGAAGCCT 
GAGAAGGCCT CGGCCGGCGC CCGGCTTCCT GTGCAGGAGA AGACGAACCT GGCCGCCTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCGGAG ATCCTGGTCC GGAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCTTTCT CCTCGGACAT CAACAACATG 
CCCCTGCAGG AGCTGTCCAA CGCCCTCATG GCAGCTGAGC GCTTCAAGGA GCACCGGGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCA GCAGCCAGCA CGGCCAAACC CCCTCCTCTG 
CCTCGCTCCA ACACAGACTC CGCCGTGGTC ATGGAGGAGG GAAGTCCTGG CGAGGTTCCT 
GTGCCAGTGG AGCCCCCAGG ATTGGAGGAC TTTGAGGCGG CGCTAGGCGT GGACAGGCGC 
ACAGACGCCT ACAGCAGGTC GTCCTCAACC TCCAGCCAGG AGGAGAAGTC ATTCCACGCA 
GAGGAGCTGG TTGCCGGGGG AATCCCCATT GAACGAGCCG TCTCCTCGGA GGGTGGCCGG 
CCCTCTGTGG ACCTCTCCTT CCAGCCCTCA CAGCCCCTGA GCAAGTCTAG CTCCTCTCCT 
GAGCTGCAGA CCCTGCAGGA CATCCTCGGG GACCCTGGGG ACAAGGCTGA CGTGGGCCGG 
CTGAGCCCTG AGGTTAAGGC CCGTTCACAG TCAGGGATCC TGGACGGGGA AAGTGCTGCC 
TGGCCAGCCT CGGGCGAAGA CAGTCGGGGC CAGCCCGAGG GTCCCTTGCC TTCCAGCTCC 
CCCCGCTCAC CCAGTGGCCT CCGGCCCCGC GGTTACACCA TCTCCGACTC GGCCCCATCA 
CGCAGGGGCA GGAGGGTAGA AAGGGATGCC TTTAAGAGCA GAGCCACAGC TTCCAGTGCA 
GAGAAAGTGC CAGGCATCAA CCCCAGTTTC GTGTTCCTGC AGCTCTACCA TTCGCCTTTC 
TTTGGCGACG AGTCAAACAA GCCAATCCTG CTGCCCAATG AGTCACAGTC CTTTGAGCGG 
TCGGTGCAGC TCCTCGACCA GATCCCATCA TACGACACCC ACAAGATCGC CGTCCTGTAT 
GTGGGAGAAG GCCAGAGCAA CAGTGAGCTC GCCATCCTGT CCAATGAGCA TGGCTCCTAC 
AGGTACACGG AGTTCCTGAC GGGCCTGGGC CGGCTCATCG AGCTCAAGGA CTGCCAGCCA 
GACAAGGTGT ACCTGGGCGG CCTGGACGTG TGTGGTGAGG ACGGCCAGTT CACCTACTGC 
TGGCACGACG ACATCATGCA AGCCGTCTTC CACATCGCCA CCCTGATGCC CACCAAGGAC 
GTGGACAAGC ATCGTTGCGA CAAGAAGCGC CACCTGGGCA ACGATTTCGT GTCCATTGTC 
TACAATGACT CTGGCGAGGA CTTCAAGCTC GGCACCATCA AGGGCCAGTT CAACTTTGTC 
CACGTGATCA TCACCCCGCT GGATTATGAG TGCAACCTGG TGTCCCTGCA GTGCAGGAAA 
GGTGGGGCCA GGGTGGGGCC GGTGTCCCTG CAGTGCAGGA AAGACATGGA GGGCCTTGTG 
GACACCAGCG TGGCCAAGAT CGTGTCTGAC CGCAACCTGC CTTTTGTGGC CCGTCAGATG 
GCCCTGCATG CAAACATGGC CTCACAGGTG CACCACAGCC GCTCCAACCC CACCGACATC 
TACCCCTCCA AGTGGATTGC CCGGCTCCGC CACATCAAGA GACTCCGCCA GCGGATCTGC 
GAGGAAGCCG CCTACTCCAA CCCCAGCCTG CCTCTGGTGC ACCCCCCATC CCATACCAAA 
GGGCCTGCAC AGACTCCAGC CGAGCCCACG CCTGGCTACG AGATGGGCCA GCGGAAGCGC 
CTCATCTCCT CTGTGGAGGA CTTCACCGAG TTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015125356.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014980842.1 Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X10, mRNA.

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CloneID	OMb54737
Clone ID Related Accession (Same CDS sequence)	XM_015125356.1
Accession Version	XM_015125356.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805823.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAAGCTCCGT GGCGGCCCTC TGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
GCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAACAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACGGCCGAA 
ATCCTGCGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTACATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCATCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCAGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGCA GGATACAGAC ATCCCTCACC AGTGCCAGCC TGGGGTCTGC AGACGAGAAC 
TCCGTGGCCC AGGCTGACGA TAGCCTGAAA AGCCTCCATC TGGAGCTCAC GGAAACCTGT 
CTGGACATGA TGGCCCGATA CGTCTTCTCC AACTTCACAG CTGTCCCGAA GAGGTCTCCT 
GTGGGCGAGT TCCTCCTGGC GGGTGGCAGG ACCAAAACCT GGCTGGTTGG GAACAAGCTT 
GTCACTGTGA CGACGAGTGT GGGAACCGGG ACCCGGTCGT TACTAGGCCT GGACTCGGGG 
GAGCTGCAGT CCGGCCCGGA GTCGAGCTCC AGCCCCGGCA TGAATGTGAG ACAGACGAAG 
GAGGCGCCAG CCAAGCTGGA GTCCCAGGCT GGGCAGCAGG TGTCCCGTGG GGCCCGGGAC 
CGGGTCCGTT CTATGTCAGG GGGCCATGGT CTTCGAGTTG GCGCCCTGGA TGTGCCGGCC 
TCCCAGTTCC CGGGCAGTGC CGCTTCTCCA GGACCACAGA CTGCACCAGC CGCGAAGCCT 
GAGAAGGCCT CGGCCGGCGC CCGGCTTCCT GTGCAGGAGA AGACGAACCT GGCCGCCTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCGGAG ATCCTGGTCC GGAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCTTTCT CCTCGGACAT CAACAACATG 
CCCCTGCAGG AGCTGTCCAA CGCCCTCATG GCAGCTGAGC GCTTCAAGGA GCACCGGGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCA GCAGCCAGCA CGGCCAAACC CCCTCCTCTG 
CCTCGCTCCA ACACAGACTC CGCCGTGGTC ATGGAGGAGG GAAGTCCTGG CGAGGTTCCT 
GTGCCAGTGG AGCCCCCAGG ATTGGAGGAC TTTGAGGCGG CGCTAGGCGT GGACAGGCGC 
ACAGACGCCT ACAGCAGGTC GTCCTCAACC TCCAGCCAGG AGGAGAAGTC ATTCCACGCA 
GAGGAGCTGG TTGCCGGGGG AATCCCCATT GAACGAGCCG TCTCCTCGGA GGGTGGCCGG 
CCCTCTGTGG ACCTCTCCTT CCAGCCCTCA CAGCCCCTGA GCAAGTCTAG CTCCTCTCCT 
GAGCTGCAGA CCCTGCAGGA CATCCTCGGG GACCCTGGGG ACAAGGCTGA CGTGGGCCGG 
CTGAGCCCTG AGGTTAAGGC CCGTTCACAG TCAGGGATCC TGGACGGGGA AAGTGCTGCC 
TGGCCAGCCT CGGGCGAAGA CAGTCGGGGC CAGCCCGAGG GTCCCTTGCC TTCCAGCTCC 
CCCCGCTCAC CCAGTGGCCT CCGGCCCCGC GGTTACACCA TCTCCGACTC GGCCCCATCA 
CGCAGGGGCA GGAGGGTAGA AAGGGATGCC TTTAAGAGCA GAGCCACAGC TTCCAGTGCA 
GAGAAAGTGC CAGGCATCAA CCCCAGTTTC GTGTTCCTGC AGCTCTACCA TTCGCCTTTC 
TTTGGCGACG AGTCAAACAA GCCAATCCTG CTGCCCAATG AGTCACAGTC CTTTGAGCGG 
TCGGTGCAGC TCCTCGACCA GATCCCATCA TACGACACCC ACAAGATCGC CGTCCTGTAT 
GTGGGAGAAG GCCAGAGCAA CAGTGAGCTC GCCATCCTGT CCAATGAGCA TGGCTCCTAC 
AGGTACACGG AGTTCCTGAC GGGCCTGGGC CGGCTCATCG AGCTCAAGGA CTGCCAGCCA 
GACAAGGTGT ACCTGGGCGG CCTGGACGTG TGTGGTGAGG ACGGCCAGTT CACCTACTGC 
TGGCACGACG ACATCATGCA AGCCGTCTTC CACATCGCCA CCCTGATGCC CACCAAGGAC 
GTGGACAAGC ATCGTTGCGA CAAGAAGCGC CACCTGGGCA ACGATTTCGT GTCCATTGTC 
TACAATGACT CTGGCGAGGA CTTCAAGCTC GGCACCATCA AGGGCCAGTT CAACTTTGTC 
CACGTGATCA TCACCCCGCT GGATTATGAG TGCAACCTGG TGTCCCTGCA GTGCAGGAAA 
GACATGGAGG GCCTTGTGGA CACCAGCGTG GCCAAGATCG TGTCTGACCG CAACCTGCCT 
TTTGTGGCCC GTCAGATGGC CCTGCATGCA AACATGGCCT CACAGGTGCA CCACAGCCGC 
TCCAACCCCA CCGACATCTA CCCCTCCAAG TGGATTGCCC GGCTCCGCCA CATCAAGAGA 
CTCCGCCAGC GGATCTGCGA GGAAGCCGCC TACTCCAACC CCAGCCTGCC TCTGGTGCAC 
CCCCCATCCC ATACCAAAGG GCCTGCACAG ACTCCAGCCG AGCCCACGCC TGGCTACGAG 
ATGGGCCAGC GGAAGCGCCT CATCTCCTCT GTGGAGGACT TCACCGAGTT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014980842.1
CDS	236..5872
Translation	MLRSGRGATCCSFSPPGRRGLLPTPPRSSPGSQAPVPRQHPGAQGVHAGLLRTTHPGGPRGPRHVTQGSPAFPLEGCLELQLPASSVAALCAAPLPEVRVALPAASGARGVRLSPRRGGPARGGAAREGCSGASWPTMAKPTSKDSGLKEKFRILFGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLSNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIIQGQGERLGVLRALFFKVIKDYPSNEDLHERLDVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCICDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTDSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKGPAQTPAEPTPGYEMGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_015125356.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015125356.1

ORF Insert Sequence:

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAAGCTCCGT GGCGGCCCTC TGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
GCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAACAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACGGCCGAA 
ATCCTGCGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTACATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCATCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCAGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGCA GGATACAGAC ATCCCTCACC AGTGCCAGCC TGGGGTCTGC AGACGAGAAC 
TCCGTGGCCC AGGCTGACGA TAGCCTGAAA AGCCTCCATC TGGAGCTCAC GGAAACCTGT 
CTGGACATGA TGGCCCGATA CGTCTTCTCC AACTTCACAG CTGTCCCGAA GAGGTCTCCT 
GTGGGCGAGT TCCTCCTGGC GGGTGGCAGG ACCAAAACCT GGCTGGTTGG GAACAAGCTT 
GTCACTGTGA CGACGAGTGT GGGAACCGGG ACCCGGTCGT TACTAGGCCT GGACTCGGGG 
GAGCTGCAGT CCGGCCCGGA GTCGAGCTCC AGCCCCGGCA TGAATGTGAG ACAGACGAAG 
GAGGCGCCAG CCAAGCTGGA GTCCCAGGCT GGGCAGCAGG TGTCCCGTGG GGCCCGGGAC 
CGGGTCCGTT CTATGTCAGG GGGCCATGGT CTTCGAGTTG GCGCCCTGGA TGTGCCGGCC 
TCCCAGTTCC CGGGCAGTGC CGCTTCTCCA GGACCACAGA CTGCACCAGC CGCGAAGCCT 
GAGAAGGCCT CGGCCGGCGC CCGGCTTCCT GTGCAGGAGA AGACGAACCT GGCCGCCTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCGGAG ATCCTGGTCC GGAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCTTTCT CCTCGGACAT CAACAACATG 
CCCCTGCAGG AGCTGTCCAA CGCCCTCATG GCAGCTGAGC GCTTCAAGGA GCACCGGGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCA GCAGCCAGCA CGGCCAAACC CCCTCCTCTG 
CCTCGCTCCA ACACAGACTC CGCCGTGGTC ATGGAGGAGG GAAGTCCTGG CGAGGTTCCT 
GTGCCAGTGG AGCCCCCAGG ATTGGAGGAC TTTGAGGCGG CGCTAGGCGT GGACAGGCGC 
ACAGACGCCT ACAGCAGGTC GTCCTCAACC TCCAGCCAGG AGGAGAAGTC ATTCCACGCA 
GAGGAGCTGG TTGCCGGGGG AATCCCCATT GAACGAGCCG TCTCCTCGGA GGGTGGCCGG 
CCCTCTGTGG ACCTCTCCTT CCAGCCCTCA CAGCCCCTGA GCAAGTCTAG CTCCTCTCCT 
GAGCTGCAGA CCCTGCAGGA CATCCTCGGG GACCCTGGGG ACAAGGCTGA CGTGGGCCGG 
CTGAGCCCTG AGGTTAAGGC CCGTTCACAG TCAGGGATCC TGGACGGGGA AAGTGCTGCC 
TGGCCAGCCT CGGGCGAAGA CAGTCGGGGC CAGCCCGAGG GTCCCTTGCC TTCCAGCTCC 
CCCCGCTCAC CCAGTGGCCT CCGGCCCCGC GGTTACACCA TCTCCGACTC GGCCCCATCA 
CGCAGGGGCA GGAGGGTAGA AAGGGATGCC TTTAAGAGCA GAGCCACAGC TTCCAGTGCA 
GAGAAAGTGC CAGGCATCAA CCCCAGTTTC GTGTTCCTGC AGCTCTACCA TTCGCCTTTC 
TTTGGCGACG AGTCAAACAA GCCAATCCTG CTGCCCAATG AGTCACAGTC CTTTGAGCGG 
TCGGTGCAGC TCCTCGACCA GATCCCATCA TACGACACCC ACAAGATCGC CGTCCTGTAT 
GTGGGAGAAG GCCAGAGCAA CAGTGAGCTC GCCATCCTGT CCAATGAGCA TGGCTCCTAC 
AGGTACACGG AGTTCCTGAC GGGCCTGGGC CGGCTCATCG AGCTCAAGGA CTGCCAGCCA 
GACAAGGTGT ACCTGGGCGG CCTGGACGTG TGTGGTGAGG ACGGCCAGTT CACCTACTGC 
TGGCACGACG ACATCATGCA AGCCGTCTTC CACATCGCCA CCCTGATGCC CACCAAGGAC 
GTGGACAAGC ATCGTTGCGA CAAGAAGCGC CACCTGGGCA ACGATTTCGT GTCCATTGTC 
TACAATGACT CTGGCGAGGA CTTCAAGCTC GGCACCATCA AGGGCCAGTT CAACTTTGTC 
CACGTGATCA TCACCCCGCT GGATTATGAG TGCAACCTGG TGTCCCTGCA GTGCAGGAAA 
GACATGGAGG GCCTTGTGGA CACCAGCGTG GCCAAGATCG TGTCTGACCG CAACCTGCCT 
TTTGTGGCCC GTCAGATGGC CCTGCATGCA AACATGGCCT CACAGGTGCA CCACAGCCGC 
TCCAACCCCA CCGACATCTA CCCCTCCAAG TGGATTGCCC GGCTCCGCCA CATCAAGAGA 
CTCCGCCAGC GGATCTGCGA GGAAGCCGCC TACTCCAACC CCAGCCTGCC TCTGGTGCAC 
CCCCCATCCC ATACCAAAGG GCCTGCACAG ACTCCAGCCG AGCCCACGCC TGGCTACGAG 
ATGGGCCAGC GGAAGCGCCT CATCTCCTCT GTGGAGGACT TCACCGAGTT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015125357.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014980843.1 Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X11, mRNA.

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CloneID	OMb54738
Clone ID Related Accession (Same CDS sequence)	XM_015125357.1
Accession Version	XM_015125357.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805823.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAAGCTCCGT GGCGGCCCTC TGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
GCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAACAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACGGCCGAA 
ATCCTGCGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTACATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCATCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCAGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGGA TACAGACATC CCTCACCAGT GCCAGCCTGG GGTCTGCAGA CGAGAACTCC 
GTGGCCCAGG CTGACGATAG CCTGAAAAGC CTCCATCTGG AGCTCACGGA AACCTGTCTG 
GACATGATGG CCCGATACGT CTTCTCCAAC TTCACAGCTG TCCCGAAGAG GTCTCCTGTG 
GGCGAGTTCC TCCTGGCGGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACTGTGACGA CGAGTGTGGG AACCGGGACC CGGTCGTTAC TAGGCCTGGA CTCGGGGGAG 
CTGCAGTCCG GCCCGGAGTC GAGCTCCAGC CCCGGCATGA ATGTGAGACA GACGAAGGAG 
GCGCCAGCCA AGCTGGAGTC CCAGGCTGGG CAGCAGGTGT CCCGTGGGGC CCGGGACCGG 
GTCCGTTCTA TGTCAGGGGG CCATGGTCTT CGAGTTGGCG CCCTGGATGT GCCGGCCTCC 
CAGTTCCCGG GCAGTGCCGC TTCTCCAGGA CCACAGACTG CACCAGCCGC GAAGCCTGAG 
AAGGCCTCGG CCGGCGCCCG GCTTCCTGTG CAGGAGAAGA CGAACCTGGC CGCCTACGTG 
CCCCTGCTGA CCCAGGGCTG GGCGGAGATC CTGGTCCGGA GGCCCACAGG GAACACCAGC 
TGGCTGATGA GCCTGGAGAA CCCGCTCAGC CCTTTCTCCT CGGACATCAA CAACATGCCC 
CTGCAGGAGC TGTCCAACGC CCTCATGGCA GCTGAGCGCT TCAAGGAGCA CCGGGACACA 
GCCCTGTACA AGTCGCTGTC GGTGCCAGCA GCCAGCACGG CCAAACCCCC TCCTCTGCCT 
CGCTCCAACA CAGACTCCGC CGTGGTCATG GAGGAGGGAA GTCCTGGCGA GGTTCCTGTG 
CCAGTGGAGC CCCCAGGATT GGAGGACTTT GAGGCGGCGC TAGGCGTGGA CAGGCGCACA 
GACGCCTACA GCAGGTCGTC CTCAACCTCC AGCCAGGAGG AGAAGTCATT CCACGCAGAG 
GAGCTGGTTG CCGGGGGAAT CCCCATTGAA CGAGCCGTCT CCTCGGAGGG TGGCCGGCCC 
TCTGTGGACC TCTCCTTCCA GCCCTCACAG CCCCTGAGCA AGTCTAGCTC CTCTCCTGAG 
CTGCAGACCC TGCAGGACAT CCTCGGGGAC CCTGGGGACA AGGCTGACGT GGGCCGGCTG 
AGCCCTGAGG TTAAGGCCCG TTCACAGTCA GGGATCCTGG ACGGGGAAAG TGCTGCCTGG 
CCAGCCTCGG GCGAAGACAG TCGGGGCCAG CCCGAGGGTC CCTTGCCTTC CAGCTCCCCC 
CGCTCACCCA GTGGCCTCCG GCCCCGCGGT TACACCATCT CCGACTCGGC CCCATCACGC 
AGGGGCAGGA GGGTAGAAAG GGATGCCTTT AAGAGCAGAG CCACAGCTTC CAGTGCAGAG 
AAAGTGCCAG GCATCAACCC CAGTTTCGTG TTCCTGCAGC TCTACCATTC GCCTTTCTTT 
GGCGACGAGT CAAACAAGCC AATCCTGCTG CCCAATGAGT CACAGTCCTT TGAGCGGTCG 
GTGCAGCTCC TCGACCAGAT CCCATCATAC GACACCCACA AGATCGCCGT CCTGTATGTG 
GGAGAAGGCC AGAGCAACAG TGAGCTCGCC ATCCTGTCCA ATGAGCATGG CTCCTACAGG 
TACACGGAGT TCCTGACGGG CCTGGGCCGG CTCATCGAGC TCAAGGACTG CCAGCCAGAC 
AAGGTGTACC TGGGCGGCCT GGACGTGTGT GGTGAGGACG GCCAGTTCAC CTACTGCTGG 
CACGACGACA TCATGCAAGC CGTCTTCCAC ATCGCCACCC TGATGCCCAC CAAGGACGTG 
GACAAGCATC GTTGCGACAA GAAGCGCCAC CTGGGCAACG ATTTCGTGTC CATTGTCTAC 
AATGACTCTG GCGAGGACTT CAAGCTCGGC ACCATCAAGG GCCAGTTCAA CTTTGTCCAC 
GTGATCATCA CCCCGCTGGA TTATGAGTGC AACCTGGTGT CCCTGCAGTG CAGGAAAGAC 
ATGGAGGGCC TTGTGGACAC CAGCGTGGCC AAGATCGTGT CTGACCGCAA CCTGCCTTTT 
GTGGCCCGTC AGATGGCCCT GCATGCAAAC ATGGCCTCAC AGGTGCACCA CAGCCGCTCC 
AACCCCACCG ACATCTACCC CTCCAAGTGG ATTGCCCGGC TCCGCCACAT CAAGAGACTC 
CGCCAGCGGA TCTGCGAGGA AGCCGCCTAC TCCAACCCCA GCCTGCCTCT GGTGCACCCC 
CCATCCCATA CCAAAGGGCC TGCACAGACT CCAGCCGAGC CCACGCCTGG CTACGAGATG 
GGCCAGCGGA AGCGCCTCAT CTCCTCTGTG GAGGACTTCA CCGAGTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014980843.1
CDS	236..5869
Translation	MLRSGRGATCCSFSPPGRRGLLPTPPRSSPGSQAPVPRQHPGAQGVHAGLLRTTHPGGPRGPRHVTQGSPAFPLEGCLELQLPASSVAALCAAPLPEVRVALPAASGARGVRLSPRRGGPARGGAAREGCSGASWPTMAKPTSKDSGLKEKFRILFGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLSNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIIQGQGERLGVLRALFFKVIKDYPSNEDLHERLDVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCICDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTDSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKGPAQTPAEPTPGYEMGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_015125357.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015125357.1

ORF Insert Sequence:

ATGCTCCGGT CAGGGCGCGG CGCTACCTGC TGCAGCTTCT CCCCTCCGGG GAGGCGCGGG 
CTCCTCCCCA CACCCCCGCG GTCCAGCCCT GGGTCCCAGG CTCCGGTTCC GCGTCAGCAT 
CCTGGCGCTC AAGGAGTTCA TGCCGGACTC CTGCGGACTA CACATCCCGG CGGCCCACGC 
GGCCCCCGTC ACGTGACGCA AGGATCGCCG GCCTTTCCGC TAGAGGGCTG CCTAGAACTA 
CAACTCCCAG CAAGCTCCGT GGCGGCCCTC TGCGCGGCAC CGCTGCCGGA AGTGCGGGTC 
GCGCTTCCGG CAGCGTCCGG GGCCAGGGGG GTTCGCCTTT CTCCGCGTCG GGGCGGCCCG 
GCGCGCGGTG GCGCGGCGAG GGAGGGGTGT TCTGGTGCGT CCTGGCCCAC CATGGCCAAA 
CCAACAAGCA AAGATTCAGG CTTGAAGGAG AAGTTTAGGA TTCTGTTTGG ACTGGGAACA 
CCGAGGCCAA ATCCCAGGTC TGCAGAGGGC AAACAGACGG AATTTATCAT CACGGCCGAA 
ATCCTGCGAG AGCTGAGTGT GGAATGCGGC CTCAGCAATC GCATCCGGAT GATAGGGCAG 
ATTTGTGAAG TCGCAAAAAC CAAGAAATTT GAAGAGCACG CAGTGGAAGC ACTCTGGAAG 
GCGGTTGCGG ATCTGTTGCA GCCAGAACGG CCGCTGGAGG CCCGGCACGC GGTGCTGGCT 
CTGCTGAAGG CCATCATTCA GGGGCAGGGC GAGCGTTTGG GGGTCCTCAG GGCCCTCTTC 
TTTAAGGTCA TCAAGGATTA CCCTTCCAAC GAAGACCTCC ACGAGAGGCT GGACGTTTTC 
AAGGCCCTCA CAGACAATGG GAGACACATC ACCTACTTGG AGGAAGAGCT GGCGGACTTT 
GTCCTGCAGT GGATGGATGT CGGCTTGTCC TCGGAATTCC TTCTGGTGCT GGTGAACTTG 
GTCAAATTCA ACAGCTGTTA CCTCGATGAG TACATCGCAA GGATGGTTCA GATGATCTGC 
CTGCTCTGCG TCCGGACCGC ATCCTCTGTG GACATAGAGG TCTCCCTGCA GGTGCTGGAC 
GCCGTGGTCT GCTACAACTG CCTGCCGGCT GAGAGCCTCC CGCTGTTCAT CGTCACCCTG 
TGCCGCACCA TCAATGTCAA GGAGCTCTGC GAGCCTTGCT GGAAGCTGAT GCGGAACCTC 
CTTGGCACCC ACCTGGGCCA CAGTGCCATC TACAACATGT GCCACCTCAT GGAGGACAGA 
GCCTACATGG AGGACGCGCC CCTGCTGAGA GGAGCCGTGT TTTTTGTGGG CATGGCTCTC 
TGGGGAGCCC ACCGGCTCTA TTCTCTCAGG AACTCGCCGA CATCGGTGCT GCCATCATTT 
TACCAGGCCA TGGCATGTCC GAACGAGGTG GTGTCCTATG AGATCGTCCT GTCCATCACC 
AGGCTCATCA AGAAGTATAG GAAGGAGCTC CAGGTGGTGA CGTGGGACAT TCTGCTGAAC 
ATCATCGAAC GGCTCCTTCA GCAGCTCCAG ACCTTGGACA GCCCGGAGCT CAGGACCATC 
GTCCATGACC TGCTGACCAC GGTGGAGGAG CTGTGTGACC AGAATGAGTT CCACGGGTCT 
CAGGAGAGAT ACTTTGAACT GGTGGAGAGA TGTGCAGACC AGAGGCCTGA GTCCTCCCTC 
CTGAACCTGA TCTCCTACAG AGCGCAGTCC ATTCACCCGG CCAAGGACGG CTGGATTCAG 
AACCTGCAGG CGCTGATGGA GAGATTCTTC AGGAGCGAGT CCCGAGGCGC CGTGCGCATC 
AAGGTGCTGG ATGTGCTGTC CTTTGTGCTG CTCATCAACA GGCAGTTCTA CGAGGAGGAG 
CTGATTAACT CAGTGGTCAT CTCTCAGCTC TCCCACATCC CCGAGGATAA AGACCATCAG 
GTCCGAAAGC TGGCCACCCA GTTGCTGGTG GACCTGGCAG AGGGCTGCCA CACACACCAC 
TTCAACAGTC TGCTGGACAT CATCGAGAAG GTGATGGCCC GCTCCCTCTC CCCACCCCCG 
GAGTTGGAAG AAAGGGATGT GGCCGCGTAC TCGGCGTCCT TGGAGGATGT GAAGACAGCT 
GTCCTGGGGC TTCTGGTCAT CCTTCAGACT AAGCTGTACA CCCTGCCTGC GAGCCATGCC 
ACGCGCGTTT ATGAGATGCT GGTCAGCCAC ATTCAGCTCC ACTACAAGCA CAGCTACACC 
CTGCCAATCG CCAGCAGCAT CCGGCTGCAG GCCTTTGACT TCCTGTTGCT GCTGCGGGCC 
GACTCACTGC ACCGCCTCGG CCTGCCCAAC AAGGATGGGG TCGTGCGGTT CAGCCCCTAC 
TGCATCTGCG ACTACATGGA GCCGGAGAGA GGCTCTGAGA AGAAGGCCAG CGGCCCCCTT 
TCTCCTCCCA CAGGGCCACC TGGCCCGGCG CCTGCAGGCC CGGCTGTGCG GCTGGGCTCC 
CTGCCCTACT CCCTGCTCTT CCGCGTCCTG CTGCAGTGCT TGAAGCAGGA GTCTGACTGG 
AAGGTGCTGA AGCTGGTTCT GGGCAGGCTG CCTGAGTCCC TGCGCTACAA AGTACTCATC 
TTTACCTCCC CTTGCAGTGT GGACCAGCTG TGCTCTGCTC TCTGCTCCAT GCTTTCAGGC 
CCAAAGACAC TGGAGCGGCT CCGAGGCGCC CCAGAAGGCT TCTCCAGAAC TGACCTGCAC 
CTGGCTGTGG TTCCAGTGCT GACGGCATTA ATCTCTTACC ATAACTACCT GGACAAAACC 
AAACAGCGCG AGATGGTCTA CTGCCTGGAG CAGGGCCTCA TCCACCGCTG TGCCAGCCAG 
TGCGTCGTAG CCTTGTCCAT CTGCAGCGTG GAGATGCCTG ACATCATGAT CAAGGCGCTG 
CCCGTCCTGG TGGTGAAGCT CACACACATC TCGGCCACAG CCAGCATGGC CGTCCCGCTG 
CTGGAGTTCC TGTCCACTCT GGCCAGGCTG CCGCAGCTCT ACAGGAACTT CGCCGCGGAG 
CAGTATGCCA GTGTGTTCGC CATCTCACTG CCGTACACCA ACCCCTCCAA GTTTAATCAG 
TACATCGTGT GTCTGGCCCA TCACGTCATA GCCATGTGGT TTATCAGGTG CCGCCTGCCC 
TTCCGGAAGG ATTTTGTCCC TTTCATCACT AAGGGCCTGC GGTCCAATGT CCTCTTGTCT 
TTTGATGACA CCCCCGAGAA GGACAGCTTC AGGGCCCGGA GTACTAGTCT CAACGAGAGG 
CCCAAGAGGA TACAGACATC CCTCACCAGT GCCAGCCTGG GGTCTGCAGA CGAGAACTCC 
GTGGCCCAGG CTGACGATAG CCTGAAAAGC CTCCATCTGG AGCTCACGGA AACCTGTCTG 
GACATGATGG CCCGATACGT CTTCTCCAAC TTCACAGCTG TCCCGAAGAG GTCTCCTGTG 
GGCGAGTTCC TCCTGGCGGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACTGTGACGA CGAGTGTGGG AACCGGGACC CGGTCGTTAC TAGGCCTGGA CTCGGGGGAG 
CTGCAGTCCG GCCCGGAGTC GAGCTCCAGC CCCGGCATGA ATGTGAGACA GACGAAGGAG 
GCGCCAGCCA AGCTGGAGTC CCAGGCTGGG CAGCAGGTGT CCCGTGGGGC CCGGGACCGG 
GTCCGTTCTA TGTCAGGGGG CCATGGTCTT CGAGTTGGCG CCCTGGATGT GCCGGCCTCC 
CAGTTCCCGG GCAGTGCCGC TTCTCCAGGA CCACAGACTG CACCAGCCGC GAAGCCTGAG 
AAGGCCTCGG CCGGCGCCCG GCTTCCTGTG CAGGAGAAGA CGAACCTGGC CGCCTACGTG 
CCCCTGCTGA CCCAGGGCTG GGCGGAGATC CTGGTCCGGA GGCCCACAGG GAACACCAGC 
TGGCTGATGA GCCTGGAGAA CCCGCTCAGC CCTTTCTCCT CGGACATCAA CAACATGCCC 
CTGCAGGAGC TGTCCAACGC CCTCATGGCA GCTGAGCGCT TCAAGGAGCA CCGGGACACA 
GCCCTGTACA AGTCGCTGTC GGTGCCAGCA GCCAGCACGG CCAAACCCCC TCCTCTGCCT 
CGCTCCAACA CAGACTCCGC CGTGGTCATG GAGGAGGGAA GTCCTGGCGA GGTTCCTGTG 
CCAGTGGAGC CCCCAGGATT GGAGGACTTT GAGGCGGCGC TAGGCGTGGA CAGGCGCACA 
GACGCCTACA GCAGGTCGTC CTCAACCTCC AGCCAGGAGG AGAAGTCATT CCACGCAGAG 
GAGCTGGTTG CCGGGGGAAT CCCCATTGAA CGAGCCGTCT CCTCGGAGGG TGGCCGGCCC 
TCTGTGGACC TCTCCTTCCA GCCCTCACAG CCCCTGAGCA AGTCTAGCTC CTCTCCTGAG 
CTGCAGACCC TGCAGGACAT CCTCGGGGAC CCTGGGGACA AGGCTGACGT GGGCCGGCTG 
AGCCCTGAGG TTAAGGCCCG TTCACAGTCA GGGATCCTGG ACGGGGAAAG TGCTGCCTGG 
CCAGCCTCGG GCGAAGACAG TCGGGGCCAG CCCGAGGGTC CCTTGCCTTC CAGCTCCCCC 
CGCTCACCCA GTGGCCTCCG GCCCCGCGGT TACACCATCT CCGACTCGGC CCCATCACGC 
AGGGGCAGGA GGGTAGAAAG GGATGCCTTT AAGAGCAGAG CCACAGCTTC CAGTGCAGAG 
AAAGTGCCAG GCATCAACCC CAGTTTCGTG TTCCTGCAGC TCTACCATTC GCCTTTCTTT 
GGCGACGAGT CAAACAAGCC AATCCTGCTG CCCAATGAGT CACAGTCCTT TGAGCGGTCG 
GTGCAGCTCC TCGACCAGAT CCCATCATAC GACACCCACA AGATCGCCGT CCTGTATGTG 
GGAGAAGGCC AGAGCAACAG TGAGCTCGCC ATCCTGTCCA ATGAGCATGG CTCCTACAGG 
TACACGGAGT TCCTGACGGG CCTGGGCCGG CTCATCGAGC TCAAGGACTG CCAGCCAGAC 
AAGGTGTACC TGGGCGGCCT GGACGTGTGT GGTGAGGACG GCCAGTTCAC CTACTGCTGG 
CACGACGACA TCATGCAAGC CGTCTTCCAC ATCGCCACCC TGATGCCCAC CAAGGACGTG 
GACAAGCATC GTTGCGACAA GAAGCGCCAC CTGGGCAACG ATTTCGTGTC CATTGTCTAC 
AATGACTCTG GCGAGGACTT CAAGCTCGGC ACCATCAAGG GCCAGTTCAA CTTTGTCCAC 
GTGATCATCA CCCCGCTGGA TTATGAGTGC AACCTGGTGT CCCTGCAGTG CAGGAAAGAC 
ATGGAGGGCC TTGTGGACAC CAGCGTGGCC AAGATCGTGT CTGACCGCAA CCTGCCTTTT 
GTGGCCCGTC AGATGGCCCT GCATGCAAAC ATGGCCTCAC AGGTGCACCA CAGCCGCTCC 
AACCCCACCG ACATCTACCC CTCCAAGTGG ATTGCCCGGC TCCGCCACAT CAAGAGACTC 
CGCCAGCGGA TCTGCGAGGA AGCCGCCTAC TCCAACCCCA GCCTGCCTCT GGTGCACCCC 
CCATCCCATA CCAAAGGGCC TGCACAGACT CCAGCCGAGC CCACGCCTGG CTACGAGATG 
GGCCAGCGGA AGCGCCTCAT CTCCTCTGTG GAGGACTTCA CCGAGTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015125358.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014980844.1 Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X12, mRNA.

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CloneID	OMb54739
Clone ID Related Accession (Same CDS sequence)	XM_015125358.1
Accession Version	XM_015125358.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805823.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAGGAT TCTGTTTGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGCA AACAGACGGA ATTTATCATC 
ACGGCCGAAA TCCTGCGAGA GCTGAGTGTG GAATGCGGCC TCAGCAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTTGCGGA TCTGTTGCAG CCAGAACGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCATTCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGG 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTCCA CGAGAGGCTG 
GACGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCGGACTTTG TCCTGCAGTG GATGGATGTC GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA CAGCTGTTAC CTCGATGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGCC TGCTCTGCGT CCGGACCGCA TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCGGTGCTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGAC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GCTGACCACG GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCAGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTACAGA GCGCAGTCCA TTCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAC 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCTCAGCTCT CCCACATCCC CGAGGATAAA 
GACCATCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGTCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGTTGGAAGA AAGGGATGTG GCCGCGTACT CGGCGTCCTT GGAGGATGTG 
AAGACAGCTG TCCTGGGGCT TCTGGTCATC CTTCAGACTA AGCTGTACAC CCTGCCTGCG 
AGCCATGCCA CGCGCGTTTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC CAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTCGGC CTGCCCAACA AGGATGGGGT CGTGCGGTTC 
AGCCCCTACT GCATCTGCGA CTACATGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCACCT GGCCCGGCGC CTGCAGGCCC GGCTGTGCGG 
CTGGGCTCCC TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTACAAA 
GTACTCATCT TTACCTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACCTGCACC TGGCTGTGGT TCCAGTGCTG ACGGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTAGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATGATC 
AAGGCGCTGC CCGTCCTGGT GGTGAAGCTC ACACACATCT CGGCCACAGC CAGCATGGCC 
GTCCCGCTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCAGCTCTA CAGGAACTTC 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCACTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT TATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGGC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATACAGACAT CCCTCACCAG TGCCAGCCTG 
GGGTCTGCAG ACGAGAACTC CGTGGCCCAG GCTGACGATA GCCTGAAAAG CCTCCATCTG 
GAGCTCACGG AAACCTGTCT GGACATGATG GCCCGATACG TCTTCTCCAA CTTCACAGCT 
GTCCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTGGCGG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACGAGTGTGG GAACCGGGAC CCGGTCGTTA 
CTAGGCCTGG ACTCGGGGGA GCTGCAGTCC GGCCCGGAGT CGAGCTCCAG CCCCGGCATG 
AATGTGAGAC AGACGAAGGA GGCGCCAGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGACCG GGTCCGTTCT ATGTCAGGGG GCCATGGTCT TCGAGTTGGC 
GCCCTGGATG TGCCGGCCTC CCAGTTCCCG GGCAGTGCCG CTTCTCCAGG ACCACAGACT 
GCACCAGCCG CGAAGCCTGA GAAGGCCTCG GCCGGCGCCC GGCTTCCTGT GCAGGAGAAG 
ACGAACCTGG CCGCCTACGT GCCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCCAACG CCCTCATGGC AGCTGAGCGC 
TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCGCTGT CGGTGCCAGC AGCCAGCACG 
GCCAAACCCC CTCCTCTGCC TCGCTCCAAC ACAGTGGCCT CTTTCTCCTC CCTGTACCAG 
TCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCCGC CGTGGTCATG 
GAGGAGGGAA GTCCTGGCGA GGTTCCTGTG CCAGTGGAGC CCCCAGGATT GGAGGACTTT 
GAGGCGGCGC TAGGCGTGGA CAGGCGCACA GACGCCTACA GCAGGTCGTC CTCAACCTCC 
AGCCAGGAGG AGAAGTCATT CCACGCAGAG GAGCTGGTTG CCGGGGGAAT CCCCATTGAA 
CGAGCCGTCT CCTCGGAGGG TGGCCGGCCC TCTGTGGACC TCTCCTTCCA GCCCTCACAG 
CCCCTGAGCA AGTCTAGCTC CTCTCCTGAG CTGCAGACCC TGCAGGACAT CCTCGGGGAC 
CCTGGGGACA AGGCTGACGT GGGCCGGCTG AGCCCTGAGG TTAAGGCCCG TTCACAGTCA 
GGGATCCTGG ACGGGGAAAG TGCTGCCTGG CCAGCCTCGG GCGAAGACAG TCGGGGCCAG 
CCCGAGGGTC CCTTGCCTTC CAGCTCCCCC CGCTCACCCA GTGGCCTCCG GCCCCGCGGT 
TACACCATCT CCGACTCGGC CCCATCACGC AGGGGCAGGA GGGTAGAAAG GGATGCCTTT 
AAGAGCAGAG CCACAGCTTC CAGTGCAGAG AAAGTGCCAG GCATCAACCC CAGTTTCGTG 
TTCCTGCAGC TCTACCATTC GCCTTTCTTT GGCGACGAGT CAAACAAGCC AATCCTGCTG 
CCCAATGAGT CACAGTCCTT TGAGCGGTCG GTGCAGCTCC TCGACCAGAT CCCATCATAC 
GACACCCACA AGATCGCCGT CCTGTATGTG GGAGAAGGCC AGAGCAACAG TGAGCTCGCC 
ATCCTGTCCA ATGAGCATGG CTCCTACAGG TACACGGAGT TCCTGACGGG CCTGGGCCGG 
CTCATCGAGC TCAAGGACTG CCAGCCAGAC AAGGTGTACC TGGGCGGCCT GGACGTGTGT 
GGTGAGGACG GCCAGTTCAC CTACTGCTGG CACGACGACA TCATGCAAGC CGTCTTCCAC 
ATCGCCACCC TGATGCCCAC CAAGGACGTG GACAAGCATC GTTGCGACAA GAAGCGCCAC 
CTGGGCAACG ATTTCGTGTC CATTGTCTAC AATGACTCTG GCGAGGACTT CAAGCTCGGC 
ACCATCAAGG GCCAGTTCAA CTTTGTCCAC GTGATCATCA CCCCGCTGGA TTATGAGTGC 
AACCTGGTGT CCCTGCAGTG CAGGAAAGGT GGGGCCAGGG TGGGGCCGGT GTCCCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAGATCGT GTCTGACCGC 
AACCTGCCTT TTGTGGCCCG TCAGATGGCC CTGCATGCAA ACATGGCCTC ACAGGTGCAC 
CACAGCCGCT CCAACCCCAC CGACATCTAC CCCTCCAAGT GGATTGCCCG GCTCCGCCAC 
ATCAAGAGAC TCCGCCAGCG GATCTGCGAG GAAGCCGCCT ACTCCAACCC CAGCCTGCCT 
CTGGTGCACC CCCCATCCCA TACCAAAGGG CCTGCACAGA CTCCAGCCGA GCCCACGCCT 
GGCTACGAGA TGGGCCAGCG GAAGCGCCTC ATCTCCTCTG TGGAGGACTT CACCGAGTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014980844.1
CDS	367..5832
Translation	MAKPTSKDSGLKEKFRILFGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLSNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIIQGQGERLGVLRALFFKVIKDYPSNEDLHERLDVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCICDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKGGARVGPVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKGPAQTPAEPTPGYEMGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_015125358.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015125358.1

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAGGAT TCTGTTTGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGCA AACAGACGGA ATTTATCATC 
ACGGCCGAAA TCCTGCGAGA GCTGAGTGTG GAATGCGGCC TCAGCAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTTGCGGA TCTGTTGCAG CCAGAACGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCATTCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGG 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTCCA CGAGAGGCTG 
GACGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCGGACTTTG TCCTGCAGTG GATGGATGTC GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA CAGCTGTTAC CTCGATGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGCC TGCTCTGCGT CCGGACCGCA TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCGGTGCTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGAC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GCTGACCACG GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCAGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTACAGA GCGCAGTCCA TTCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAC 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCTCAGCTCT CCCACATCCC CGAGGATAAA 
GACCATCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGTCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGTTGGAAGA AAGGGATGTG GCCGCGTACT CGGCGTCCTT GGAGGATGTG 
AAGACAGCTG TCCTGGGGCT TCTGGTCATC CTTCAGACTA AGCTGTACAC CCTGCCTGCG 
AGCCATGCCA CGCGCGTTTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC CAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTCGGC CTGCCCAACA AGGATGGGGT CGTGCGGTTC 
AGCCCCTACT GCATCTGCGA CTACATGGAG CCGGAGAGAG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCACCT GGCCCGGCGC CTGCAGGCCC GGCTGTGCGG 
CTGGGCTCCC TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTACAAA 
GTACTCATCT TTACCTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACCTGCACC TGGCTGTGGT TCCAGTGCTG ACGGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTAGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATGATC 
AAGGCGCTGC CCGTCCTGGT GGTGAAGCTC ACACACATCT CGGCCACAGC CAGCATGGCC 
GTCCCGCTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCAGCTCTA CAGGAACTTC 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCACTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT TATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGGC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATACAGACAT CCCTCACCAG TGCCAGCCTG 
GGGTCTGCAG ACGAGAACTC CGTGGCCCAG GCTGACGATA GCCTGAAAAG CCTCCATCTG 
GAGCTCACGG AAACCTGTCT GGACATGATG GCCCGATACG TCTTCTCCAA CTTCACAGCT 
GTCCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTGGCGG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACGAGTGTGG GAACCGGGAC CCGGTCGTTA 
CTAGGCCTGG ACTCGGGGGA GCTGCAGTCC GGCCCGGAGT CGAGCTCCAG CCCCGGCATG 
AATGTGAGAC AGACGAAGGA GGCGCCAGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGACCG GGTCCGTTCT ATGTCAGGGG GCCATGGTCT TCGAGTTGGC 
GCCCTGGATG TGCCGGCCTC CCAGTTCCCG GGCAGTGCCG CTTCTCCAGG ACCACAGACT 
GCACCAGCCG CGAAGCCTGA GAAGGCCTCG GCCGGCGCCC GGCTTCCTGT GCAGGAGAAG 
ACGAACCTGG CCGCCTACGT GCCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCCAACG CCCTCATGGC AGCTGAGCGC 
TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCGCTGT CGGTGCCAGC AGCCAGCACG 
GCCAAACCCC CTCCTCTGCC TCGCTCCAAC ACAGTGGCCT CTTTCTCCTC CCTGTACCAG 
TCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCCGC CGTGGTCATG 
GAGGAGGGAA GTCCTGGCGA GGTTCCTGTG CCAGTGGAGC CCCCAGGATT GGAGGACTTT 
GAGGCGGCGC TAGGCGTGGA CAGGCGCACA GACGCCTACA GCAGGTCGTC CTCAACCTCC 
AGCCAGGAGG AGAAGTCATT CCACGCAGAG GAGCTGGTTG CCGGGGGAAT CCCCATTGAA 
CGAGCCGTCT CCTCGGAGGG TGGCCGGCCC TCTGTGGACC TCTCCTTCCA GCCCTCACAG 
CCCCTGAGCA AGTCTAGCTC CTCTCCTGAG CTGCAGACCC TGCAGGACAT CCTCGGGGAC 
CCTGGGGACA AGGCTGACGT GGGCCGGCTG AGCCCTGAGG TTAAGGCCCG TTCACAGTCA 
GGGATCCTGG ACGGGGAAAG TGCTGCCTGG CCAGCCTCGG GCGAAGACAG TCGGGGCCAG 
CCCGAGGGTC CCTTGCCTTC CAGCTCCCCC CGCTCACCCA GTGGCCTCCG GCCCCGCGGT 
TACACCATCT CCGACTCGGC CCCATCACGC AGGGGCAGGA GGGTAGAAAG GGATGCCTTT 
AAGAGCAGAG CCACAGCTTC CAGTGCAGAG AAAGTGCCAG GCATCAACCC CAGTTTCGTG 
TTCCTGCAGC TCTACCATTC GCCTTTCTTT GGCGACGAGT CAAACAAGCC AATCCTGCTG 
CCCAATGAGT CACAGTCCTT TGAGCGGTCG GTGCAGCTCC TCGACCAGAT CCCATCATAC 
GACACCCACA AGATCGCCGT CCTGTATGTG GGAGAAGGCC AGAGCAACAG TGAGCTCGCC 
ATCCTGTCCA ATGAGCATGG CTCCTACAGG TACACGGAGT TCCTGACGGG CCTGGGCCGG 
CTCATCGAGC TCAAGGACTG CCAGCCAGAC AAGGTGTACC TGGGCGGCCT GGACGTGTGT 
GGTGAGGACG GCCAGTTCAC CTACTGCTGG CACGACGACA TCATGCAAGC CGTCTTCCAC 
ATCGCCACCC TGATGCCCAC CAAGGACGTG GACAAGCATC GTTGCGACAA GAAGCGCCAC 
CTGGGCAACG ATTTCGTGTC CATTGTCTAC AATGACTCTG GCGAGGACTT CAAGCTCGGC 
ACCATCAAGG GCCAGTTCAA CTTTGTCCAC GTGATCATCA CCCCGCTGGA TTATGAGTGC 
AACCTGGTGT CCCTGCAGTG CAGGAAAGGT GGGGCCAGGG TGGGGCCGGT GTCCCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAGATCGT GTCTGACCGC 
AACCTGCCTT TTGTGGCCCG TCAGATGGCC CTGCATGCAA ACATGGCCTC ACAGGTGCAC 
CACAGCCGCT CCAACCCCAC CGACATCTAC CCCTCCAAGT GGATTGCCCG GCTCCGCCAC 
ATCAAGAGAC TCCGCCAGCG GATCTGCGAG GAAGCCGCCT ACTCCAACCC CAGCCTGCCT 
CTGGTGCACC CCCCATCCCA TACCAAAGGG CCTGCACAGA CTCCAGCCGA GCCCACGCCT 
GGCTACGAGA TGGGCCAGCG GAAGCGCCTC ATCTCCTCTG TGGAGGACTT CACCGAGTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015125359.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014980845.1 Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X13, mRNA.

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CloneID	OMb54740
Clone ID Related Accession (Same CDS sequence)	XM_015125359.1
Accession Version	XM_015125359.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	tuberin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805823.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTCG GCTTGTCCTC GGAATTCCTT CTGGTGCTGG TGAACTTGGT CAAATTCAAC 
AGCTGTTACC TCGATGAGTA CATCGCAAGG ATGGTTCAGA TGATCTGCCT GCTCTGCGTC 
CGGACCGCAT CCTCTGTGGA CATAGAGGTC TCCCTGCAGG TGCTGGACGC CGTGGTCTGC 
TACAACTGCC TGCCGGCTGA GAGCCTCCCG CTGTTCATCG TCACCCTGTG CCGCACCATC 
AATGTCAAGG AGCTCTGCGA GCCTTGCTGG AAGCTGATGC GGAACCTCCT TGGCACCCAC 
CTGGGCCACA GTGCCATCTA CAACATGTGC CACCTCATGG AGGACAGAGC CTACATGGAG 
GACGCGCCCC TGCTGAGAGG AGCCGTGTTT TTTGTGGGCA TGGCTCTCTG GGGAGCCCAC 
CGGCTCTATT CTCTCAGGAA CTCGCCGACA TCGGTGCTGC CATCATTTTA CCAGGCCATG 
GCATGTCCGA ACGAGGTGGT GTCCTATGAG ATCGTCCTGT CCATCACCAG GCTCATCAAG 
AAGTATAGGA AGGAGCTCCA GGTGGTGACG TGGGACATTC TGCTGAACAT CATCGAACGG 
CTCCTTCAGC AGCTCCAGAC CTTGGACAGC CCGGAGCTCA GGACCATCGT CCATGACCTG 
CTGACCACGG TGGAGGAGCT GTGTGACCAG AATGAGTTCC ACGGGTCTCA GGAGAGATAC 
TTTGAACTGG TGGAGAGATG TGCAGACCAG AGGCCTGAGT CCTCCCTCCT GAACCTGATC 
TCCTACAGAG CGCAGTCCAT TCACCCGGCC AAGGACGGCT GGATTCAGAA CCTGCAGGCG 
CTGATGGAGA GATTCTTCAG GAGCGAGTCC CGAGGCGCCG TGCGCATCAA GGTGCTGGAT 
GTGCTGTCCT TTGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT GATTAACTCA 
GTGGTCATCT CTCAGCTCTC CCACATCCCC GAGGATAAAG ACCATCAGGT CCGAAAGCTG 
GCCACCCAGT TGCTGGTGGA CCTGGCAGAG GGCTGCCACA CACACCACTT CAACAGTCTG 
CTGGACATCA TCGAGAAGGT GATGGCCCGC TCCCTCTCCC CACCCCCGGA GTTGGAAGAA 
AGGGATGTGG CCGCGTACTC GGCGTCCTTG GAGGATGTGA AGACAGCTGT CCTGGGGCTT 
CTGGTCATCC TTCAGACTAA GCTGTACACC CTGCCTGCGA GCCATGCCAC GCGCGTTTAT 
GAGATGCTGG TCAGCCACAT TCAGCTCCAC TACAAGCACA GCTACACCCT GCCAATCGCC 
AGCAGCATCC GGCTGCAGGC CTTTGACTTC CTGTTGCTGC TGCGGGCCGA CTCACTGCAC 
CGCCTCGGCC TGCCCAACAA GGATGGGGTC GTGCGGTTCA GCCCCTACTG CATCTGCGAC 
TACATGGAGC CGGAGAGAGG CTCTGAGAAG AAGGCCAGCG GCCCCCTTTC TCCTCCCACA 
GGGCCACCTG GCCCGGCGCC TGCAGGCCCG GCTGTGCGGC TGGGCTCCCT GCCCTACTCC 
CTGCTCTTCC GCGTCCTGCT GCAGTGCTTG AAGCAGGAGT CTGACTGGAA GGTGCTGAAG 
CTGGTTCTGG GCAGGCTGCC TGAGTCCCTG CGCTACAAAG TACTCATCTT TACCTCCCCT 
TGCAGTGTGG ACCAGCTGTG CTCTGCTCTC TGCTCCATGC TTTCAGGCCC AAAGACACTG 
GAGCGGCTCC GAGGCGCCCC AGAAGGCTTC TCCAGAACTG ACCTGCACCT GGCTGTGGTT 
CCAGTGCTGA CGGCATTAAT CTCTTACCAT AACTACCTGG ACAAAACCAA ACAGCGCGAG 
ATGGTCTACT GCCTGGAGCA GGGCCTCATC CACCGCTGTG CCAGCCAGTG CGTCGTAGCC 
TTGTCCATCT GCAGCGTGGA GATGCCTGAC ATCATGATCA AGGCGCTGCC CGTCCTGGTG 
GTGAAGCTCA CACACATCTC GGCCACAGCC AGCATGGCCG TCCCGCTGCT GGAGTTCCTG 
TCCACTCTGG CCAGGCTGCC GCAGCTCTAC AGGAACTTCG CCGCGGAGCA GTATGCCAGT 
GTGTTCGCCA TCTCACTGCC GTACACCAAC CCCTCCAAGT TTAATCAGTA CATCGTGTGT 
CTGGCCCATC ACGTCATAGC CATGTGGTTT ATCAGGTGCC GCCTGCCCTT CCGGAAGGAT 
TTTGTCCCTT TCATCACTAA GGGCCTGCGG TCCAATGTCC TCTTGTCTTT TGATGACACC 
CCCGAGAAGG ACAGCTTCAG GGCCCGGAGT ACTAGTCTCA ACGAGAGGCC CAAGAGTCTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAATTCAAG 
GAGAGCTCTG CAGCCGAGGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGCAGGA TACAGACATC CCTCACCAGT GCCAGCCTGG GGTCTGCAGA CGAGAACTCC 
GTGGCCCAGG CTGACGATAG CCTGAAAAGC CTCCATCTGG AGCTCACGGA AACCTGTCTG 
GACATGATGG CCCGATACGT CTTCTCCAAC TTCACAGCTG TCCCGAAGAG GTCTCCTGTG 
GGCGAGTTCC TCCTGGCGGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACTGTGACGA CGAGTGTGGG AACCGGGACC CGGTCGTTAC TAGGCCTGGA CTCGGGGGAG 
CTGCAGTCCG GCCCGGAGTC GAGCTCCAGC CCCGGCATGA ATGTGAGACA GACGAAGGAG 
GCGCCAGCCA AGCTGGAGTC CCAGGCTGGG CAGCAGGTGT CCCGTGGGGC CCGGGACCGG 
GTCCGTTCTA TGTCAGGGGG CCATGGTCTT CGAGTTGGCG CCCTGGATGT GCCGGCCTCC 
CAGTTCCCGG GCAGTGCCGC TTCTCCAGGA CCACAGACTG CACCAGCCGC GAAGCCTGAG 
AAGGCCTCGG CCGGCGCCCG GCTTCCTGTG CAGGAGAAGA CGAACCTGGC CGCCTACGTG 
CCCCTGCTGA CCCAGGGCTG GGCGGAGATC CTGGTCCGGA GGCCCACAGG GAACACCAGC 
TGGCTGATGA GCCTGGAGAA CCCGCTCAGC CCTTTCTCCT CGGACATCAA CAACATGCCC 
CTGCAGGAGC TGTCCAACGC CCTCATGGCA GCTGAGCGCT TCAAGGAGCA CCGGGACACA 
GCCCTGTACA AGTCGCTGTC GGTGCCAGCA GCCAGCACGG CCAAACCCCC TCCTCTGCCT 
CGCTCCAACA CAGTGGCCTC TTTCTCCTCC CTGTACCAGT CCAGCTGCCA AGGACAGCTG 
CACAGGAGCG TTTCCTGGGC AGACTCCGCC GTGGTCATGG AGGAGGGAAG TCCTGGCGAG 
GTTCCTGTGC CAGTGGAGCC CCCAGGATTG GAGGACTTTG AGGCGGCGCT AGGCGTGGAC 
AGGCGCACAG ACGCCTACAG CAGGTCGTCC TCAACCTCCA GCCAGGAGGA GAAGTCATTC 
CACGCAGAGG AGCTGGTTGC CGGGGGAATC CCCATTGAAC GAGCCGTCTC CTCGGAGGGT 
GGCCGGCCCT CTGTGGACCT CTCCTTCCAG CCCTCACAGC CCCTGAGCAA GTCTAGCTCC 
TCTCCTGAGC TGCAGACCCT GCAGGACATC CTCGGGGACC CTGGGGACAA GGCTGACGTG 
GGCCGGCTGA GCCCTGAGGT TAAGGCCCGT TCACAGTCAG GGATCCTGGA CGGGGAAAGT 
GCTGCCTGGC CAGCCTCGGG CGAAGACAGT CGGGGCCAGC CCGAGGGTCC CTTGCCTTCC 
AGCTCCCCCC GCTCACCCAG TGGCCTCCGG CCCCGCGGTT ACACCATCTC CGACTCGGCC 
CCATCACGCA GGGGCAGGAG GGTAGAAAGG GATGCCTTTA AGAGCAGAGC CACAGCTTCC 
AGTGCAGAGA AAGTGCCAGG CATCAACCCC AGTTTCGTGT TCCTGCAGCT CTACCATTCG 
CCTTTCTTTG GCGACGAGTC AAACAAGCCA ATCCTGCTGC CCAATGAGTC ACAGTCCTTT 
GAGCGGTCGG TGCAGCTCCT CGACCAGATC CCATCATACG ACACCCACAA GATCGCCGTC 
CTGTATGTGG GAGAAGGCCA GAGCAACAGT GAGCTCGCCA TCCTGTCCAA TGAGCATGGC 
TCCTACAGGT ACACGGAGTT CCTGACGGGC CTGGGCCGGC TCATCGAGCT CAAGGACTGC 
CAGCCAGACA AGGTGTACCT GGGCGGCCTG GACGTGTGTG GTGAGGACGG CCAGTTCACC 
TACTGCTGGC ACGACGACAT CATGCAAGCC GTCTTCCACA TCGCCACCCT GATGCCCACC 
AAGGACGTGG ACAAGCATCG TTGCGACAAG AAGCGCCACC TGGGCAACGA TTTCGTGTCC 
ATTGTCTACA ATGACTCTGG CGAGGACTTC AAGCTCGGCA CCATCAAGGG CCAGTTCAAC 
TTTGTCCACG TGATCATCAC CCCGCTGGAT TATGAGTGCA ACCTGGTGTC CCTGCAGTGC 
AGGAAAGGTG GGGCCAGGGT GGGGCCGGTG TCCCTGCAGT GCAGGAAAGA CATGGAGGGC 
CTTGTGGACA CCAGCGTGGC CAAGATCGTG TCTGACCGCA ACCTGCCTTT TGTGGCCCGT 
CAGATGGCCC TGCATGCAAA CATGGCCTCA CAGGTGCACC ACAGCCGCTC CAACCCCACC 
GACATCTACC CCTCCAAGTG GATTGCCCGG CTCCGCCACA TCAAGAGACT CCGCCAGCGG 
ATCTGCGAGG AAGCCGCCTA CTCCAACCCC AGCCTGCCTC TGGTGCACCC CCCATCCCAT 
ACCAAAGGGC CTGCACAGAC TCCAGCCGAG CCCACGCCTG GCTACGAGAT GGGCCAGCGG 
AAGCGCCTCA TCTCCTCTGT GGAGGACTTC ACCGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014980845.1
CDS	574..5538
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCICDYMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIMIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPQLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKSLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGMNVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFPGSAASPGPQTAPAAKPEKASAGARLPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVPVEPPGLEDFEAALGVDRRTDAYSRSSSTSSQEEKSFHAEELVAGGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGILDGESAAWPASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGRRVERDAFKSRATASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKGGARVGPVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKGPAQTPAEPTPGYEMGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_015125359.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta tuberous sclerosis 2 (TSC2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015125359.1

ORF Insert Sequence:

ATGGATGTCG GCTTGTCCTC GGAATTCCTT CTGGTGCTGG TGAACTTGGT CAAATTCAAC 
AGCTGTTACC TCGATGAGTA CATCGCAAGG ATGGTTCAGA TGATCTGCCT GCTCTGCGTC 
CGGACCGCAT CCTCTGTGGA CATAGAGGTC TCCCTGCAGG TGCTGGACGC CGTGGTCTGC 
TACAACTGCC TGCCGGCTGA GAGCCTCCCG CTGTTCATCG TCACCCTGTG CCGCACCATC 
AATGTCAAGG AGCTCTGCGA GCCTTGCTGG AAGCTGATGC GGAACCTCCT TGGCACCCAC 
CTGGGCCACA GTGCCATCTA CAACATGTGC CACCTCATGG AGGACAGAGC CTACATGGAG 
GACGCGCCCC TGCTGAGAGG AGCCGTGTTT TTTGTGGGCA TGGCTCTCTG GGGAGCCCAC 
CGGCTCTATT CTCTCAGGAA CTCGCCGACA TCGGTGCTGC CATCATTTTA CCAGGCCATG 
GCATGTCCGA ACGAGGTGGT GTCCTATGAG ATCGTCCTGT CCATCACCAG GCTCATCAAG 
AAGTATAGGA AGGAGCTCCA GGTGGTGACG TGGGACATTC TGCTGAACAT CATCGAACGG 
CTCCTTCAGC AGCTCCAGAC CTTGGACAGC CCGGAGCTCA GGACCATCGT CCATGACCTG 
CTGACCACGG TGGAGGAGCT GTGTGACCAG AATGAGTTCC ACGGGTCTCA GGAGAGATAC 
TTTGAACTGG TGGAGAGATG TGCAGACCAG AGGCCTGAGT CCTCCCTCCT GAACCTGATC 
TCCTACAGAG CGCAGTCCAT TCACCCGGCC AAGGACGGCT GGATTCAGAA CCTGCAGGCG 
CTGATGGAGA GATTCTTCAG GAGCGAGTCC CGAGGCGCCG TGCGCATCAA GGTGCTGGAT 
GTGCTGTCCT TTGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT GATTAACTCA 
GTGGTCATCT CTCAGCTCTC CCACATCCCC GAGGATAAAG ACCATCAGGT CCGAAAGCTG 
GCCACCCAGT TGCTGGTGGA CCTGGCAGAG GGCTGCCACA CACACCACTT CAACAGTCTG 
CTGGACATCA TCGAGAAGGT GATGGCCCGC TCCCTCTCCC CACCCCCGGA GTTGGAAGAA 
AGGGATGTGG CCGCGTACTC GGCGTCCTTG GAGGATGTGA AGACAGCTGT CCTGGGGCTT 
CTGGTCATCC TTCAGACTAA GCTGTACACC CTGCCTGCGA GCCATGCCAC GCGCGTTTAT 
GAGATGCTGG TCAGCCACAT TCAGCTCCAC TACAAGCACA GCTACACCCT GCCAATCGCC 
AGCAGCATCC GGCTGCAGGC CTTTGACTTC CTGTTGCTGC TGCGGGCCGA CTCACTGCAC 
CGCCTCGGCC TGCCCAACAA GGATGGGGTC GTGCGGTTCA GCCCCTACTG CATCTGCGAC 
TACATGGAGC CGGAGAGAGG CTCTGAGAAG AAGGCCAGCG GCCCCCTTTC TCCTCCCACA 
GGGCCACCTG GCCCGGCGCC TGCAGGCCCG GCTGTGCGGC TGGGCTCCCT GCCCTACTCC 
CTGCTCTTCC GCGTCCTGCT GCAGTGCTTG AAGCAGGAGT CTGACTGGAA GGTGCTGAAG 
CTGGTTCTGG GCAGGCTGCC TGAGTCCCTG CGCTACAAAG TACTCATCTT TACCTCCCCT 
TGCAGTGTGG ACCAGCTGTG CTCTGCTCTC TGCTCCATGC TTTCAGGCCC AAAGACACTG 
GAGCGGCTCC GAGGCGCCCC AGAAGGCTTC TCCAGAACTG ACCTGCACCT GGCTGTGGTT 
CCAGTGCTGA CGGCATTAAT CTCTTACCAT AACTACCTGG ACAAAACCAA ACAGCGCGAG 
ATGGTCTACT GCCTGGAGCA GGGCCTCATC CACCGCTGTG CCAGCCAGTG CGTCGTAGCC 
TTGTCCATCT GCAGCGTGGA GATGCCTGAC ATCATGATCA AGGCGCTGCC CGTCCTGGTG 
GTGAAGCTCA CACACATCTC GGCCACAGCC AGCATGGCCG TCCCGCTGCT GGAGTTCCTG 
TCCACTCTGG CCAGGCTGCC GCAGCTCTAC AGGAACTTCG CCGCGGAGCA GTATGCCAGT 
GTGTTCGCCA TCTCACTGCC GTACACCAAC CCCTCCAAGT TTAATCAGTA CATCGTGTGT 
CTGGCCCATC ACGTCATAGC CATGTGGTTT ATCAGGTGCC GCCTGCCCTT CCGGAAGGAT 
TTTGTCCCTT TCATCACTAA GGGCCTGCGG TCCAATGTCC TCTTGTCTTT TGATGACACC 
CCCGAGAAGG ACAGCTTCAG GGCCCGGAGT ACTAGTCTCA ACGAGAGGCC CAAGAGTCTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAATTCAAG 
GAGAGCTCTG CAGCCGAGGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGCAGGA TACAGACATC CCTCACCAGT GCCAGCCTGG GGTCTGCAGA CGAGAACTCC 
GTGGCCCAGG CTGACGATAG CCTGAAAAGC CTCCATCTGG AGCTCACGGA AACCTGTCTG 
GACATGATGG CCCGATACGT CTTCTCCAAC TTCACAGCTG TCCCGAAGAG GTCTCCTGTG 
GGCGAGTTCC TCCTGGCGGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACTGTGACGA CGAGTGTGGG AACCGGGACC CGGTCGTTAC TAGGCCTGGA CTCGGGGGAG 
CTGCAGTCCG GCCCGGAGTC GAGCTCCAGC CCCGGCATGA ATGTGAGACA GACGAAGGAG 
GCGCCAGCCA AGCTGGAGTC CCAGGCTGGG CAGCAGGTGT CCCGTGGGGC CCGGGACCGG 
GTCCGTTCTA TGTCAGGGGG CCATGGTCTT CGAGTTGGCG CCCTGGATGT GCCGGCCTCC 
CAGTTCCCGG GCAGTGCCGC TTCTCCAGGA CCACAGACTG CACCAGCCGC GAAGCCTGAG 
AAGGCCTCGG CCGGCGCCCG GCTTCCTGTG CAGGAGAAGA CGAACCTGGC CGCCTACGTG 
CCCCTGCTGA CCCAGGGCTG GGCGGAGATC CTGGTCCGGA GGCCCACAGG GAACACCAGC 
TGGCTGATGA GCCTGGAGAA CCCGCTCAGC CCTTTCTCCT CGGACATCAA CAACATGCCC 
CTGCAGGAGC TGTCCAACGC CCTCATGGCA GCTGAGCGCT TCAAGGAGCA CCGGGACACA 
GCCCTGTACA AGTCGCTGTC GGTGCCAGCA GCCAGCACGG CCAAACCCCC TCCTCTGCCT 
CGCTCCAACA CAGTGGCCTC TTTCTCCTCC CTGTACCAGT CCAGCTGCCA AGGACAGCTG 
CACAGGAGCG TTTCCTGGGC AGACTCCGCC GTGGTCATGG AGGAGGGAAG TCCTGGCGAG 
GTTCCTGTGC CAGTGGAGCC CCCAGGATTG GAGGACTTTG AGGCGGCGCT AGGCGTGGAC 
AGGCGCACAG ACGCCTACAG CAGGTCGTCC TCAACCTCCA GCCAGGAGGA GAAGTCATTC 
CACGCAGAGG AGCTGGTTGC CGGGGGAATC CCCATTGAAC GAGCCGTCTC CTCGGAGGGT 
GGCCGGCCCT CTGTGGACCT CTCCTTCCAG CCCTCACAGC CCCTGAGCAA GTCTAGCTCC 
TCTCCTGAGC TGCAGACCCT GCAGGACATC CTCGGGGACC CTGGGGACAA GGCTGACGTG 
GGCCGGCTGA GCCCTGAGGT TAAGGCCCGT TCACAGTCAG GGATCCTGGA CGGGGAAAGT 
GCTGCCTGGC CAGCCTCGGG CGAAGACAGT CGGGGCCAGC CCGAGGGTCC CTTGCCTTCC 
AGCTCCCCCC GCTCACCCAG TGGCCTCCGG CCCCGCGGTT ACACCATCTC CGACTCGGCC 
CCATCACGCA GGGGCAGGAG GGTAGAAAGG GATGCCTTTA AGAGCAGAGC CACAGCTTCC 
AGTGCAGAGA AAGTGCCAGG CATCAACCCC AGTTTCGTGT TCCTGCAGCT CTACCATTCG 
CCTTTCTTTG GCGACGAGTC AAACAAGCCA ATCCTGCTGC CCAATGAGTC ACAGTCCTTT 
GAGCGGTCGG TGCAGCTCCT CGACCAGATC CCATCATACG ACACCCACAA GATCGCCGTC 
CTGTATGTGG GAGAAGGCCA GAGCAACAGT GAGCTCGCCA TCCTGTCCAA TGAGCATGGC 
TCCTACAGGT ACACGGAGTT CCTGACGGGC CTGGGCCGGC TCATCGAGCT CAAGGACTGC 
CAGCCAGACA AGGTGTACCT GGGCGGCCTG GACGTGTGTG GTGAGGACGG CCAGTTCACC 
TACTGCTGGC ACGACGACAT CATGCAAGCC GTCTTCCACA TCGCCACCCT GATGCCCACC 
AAGGACGTGG ACAAGCATCG TTGCGACAAG AAGCGCCACC TGGGCAACGA TTTCGTGTCC 
ATTGTCTACA ATGACTCTGG CGAGGACTTC AAGCTCGGCA CCATCAAGGG CCAGTTCAAC 
TTTGTCCACG TGATCATCAC CCCGCTGGAT TATGAGTGCA ACCTGGTGTC CCTGCAGTGC 
AGGAAAGGTG GGGCCAGGGT GGGGCCGGTG TCCCTGCAGT GCAGGAAAGA CATGGAGGGC 
CTTGTGGACA CCAGCGTGGC CAAGATCGTG TCTGACCGCA ACCTGCCTTT TGTGGCCCGT 
CAGATGGCCC TGCATGCAAA CATGGCCTCA CAGGTGCACC ACAGCCGCTC CAACCCCACC 
GACATCTACC CCTCCAAGTG GATTGCCCGG CTCCGCCACA TCAAGAGACT CCGCCAGCGG 
ATCTGCGAGG AAGCCGCCTA CTCCAACCCC AGCCTGCCTC TGGTGCACCC CCCATCCCAT 
ACCAAAGGGC CTGCACAGAC TCCAGCCGAG CCCACGCCTG GCTACGAGAT GGGCCAGCGG 
AAGCGCCTCA TCTCCTCTGT GGAGGACTTC ACCGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TSC2
Entrez Gene ID	694965
Full Name	TSC complex subunit 2
Gene Type	protein-coding
Organism	Macaca mulatta(Rhesus monkey)

Pan troglodytes (chimpanzee)	TSC2	XP_523477.3
Mus musculus (house mouse)	Tsc2	NP_001034452.1
Rattus norvegicus (Norway rat)	Tsc2	NP_036812.2
Gallus gallus (chicken)	TSC2	XP_003642168.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011123	XP_309523.4
Canis lupus familiaris (dog)	TSC2	XP_852047.2
Bos taurus (cattle)	TSC2	XP_003587856.2
Drosophila melanogaster (fruit fly)	gig	NP_524177.1
Homo sapiens (human)	TSC2	NP_000539.2
	TSC2	XP_001083256.1
Danio rerio (zebrafish)	tsc2	XP_695912.6
Xenopus tropicalis (tropical clawed frog)	tsc2	NP_001123795.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TSC2 cDNA ORF clone, Macaca mulatta(Rhesus monkey)

Gene

Clones

gRNAs

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