WEE1 cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin)

Gene
Clones
gRNAs

Summary

Gene Symbol	WEE1
Entrez Gene ID	108495049
Full Name	WEE1 G2 checkpoint kinase
Gene Type	protein-coding
Organism	Lepidothrix coronata(blue-crowned manakin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017810292.1	XP_017665781.1	wee1-like protein kinase

Pathways from BioSystems
Gene Ontology

The following WEE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WEE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLe48441	XM_017810292.1 Latest version!	Lepidothrix coronata WEE1 G2 checkpoint kinase (WEE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WEE1 ( XM_017810292.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017665781.1 Lepidothrix coronata WEE1 G2 checkpoint kinase (WEE1), mRNA.

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CloneID	OLe48441
Clone ID Related Accession (Same CDS sequence)	XM_017810292.1
Accession Version	XM_017810292.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	wee1-like protein kinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690181.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCC TCAGCCTGCA GCGGGCAGCG CCGCCCCGGC GCGTCTCGGC ACGGCGGGCG 
GCCGCCCCGA TCCGGCAGAA GCTGCTGTTC CTGAGCGGCC ACAGCGACTG CGAGGAGGAG 
GAGGAGGAAG AGGAGGAGGA AGGCGGCAGC AGCAGCGCCA ACAGCACCGG CGAGGACTCG 
GCGTTCCAGG AGGCGGACTC GCCGCTCTCG GCCGCGCGCA CCCCGGCGCG GAGCGAGCCG 
CCCCTGATGG AAGAGGAGGA GGAGGAGATG GAGCCGGCGG CGGCGCCGCT GCCGGACGAG 
GGGGACTCGT GGGAGGAGGA AGGGTTCGGC TCCTCGCCGG TCAAGTCGCC CGGAGCCTAT 
TTCCTGCTCG ACTCGCCCTC GCCGCTGGCC CCGCACAAGG GCCGCCGCTG CTGCGAGCGC 
TCCCCGCCGC ACCCGGCGGC CGGGGGATAC CGGGGGCCGC GAGGCGAGGA GCCCGGCTCG 
CCGCTGCCCG ACTACCCGGG CACGCCGCCG CACAAGACCC TGCGCAAGCT GCGCCTCTTC 
GACACGCCGC ACACGCCCAA GAGCTTGCTT TCTAAAGCAC AAGGACTAGG CTCCAGCTCA 
GTCAAACTTC GAGGGGGCTC ACTGTTTATG AATGTTGGGA AATCTGAGAA GCAAGAAGAA 
GATTTTAGAC AAACACCTCA TGTGAACATC AATCCCTTCA CTCCCGACTC CATGTTCCTT 
CACAACTCTG AAGGAAAATG TCGTAGGAGG AAGAGGATAC ACTGGAATGA CTCTTGTGGA 
GAGGACATGG AACCAAGTGA TGGAGAGCCT GAAGAGGAAA CCATGAGACC TGCTAAGAGG 
ATAACAATAA CAGAAAGTAA TATGAAGTCA CGGTATGCAA CTGAATTTCA TGAACTGGAG 
AAGATTGGAT CTGGTGAATT TGGTTCTGTG TTTAAATGTG TGAAGAGGTT AGATGGCTGC 
ATTTATGCAA TAAAGCGATC CAAGAAACCC TTGGCTGGCT CAGTGGATGA GCAGAATGCT 
TTAAGAGAGG TCTATGCACA TGCAGTTCTG GGACAGCATT CTCATGTAGT GAGGTACTAC 
TCTGCATGGG CAGAAGATGA TCATATGCTT ATACAGAATG AATATTGCAA TGGTGGCAGT 
TTAGCAGATG CCATAAGTGA AAATTACAGG AATATGCGTT ACTTTACTGA ACCAGAACTG 
AAGGACCTGC TACTTCAAGT GGCTCGAGGT TTAAAATACA TACATTCAAT GTCACTGGTA 
CACATGGATA TCAAACCTAG TAATATTTTC ATATCTAGGA CATCAGTCCC GAGTATAACT 
TTAGAAGAAG GTGATGATGA TGACTGGTCA TCTGATAGAG TCATATTTAA AATAGGTGAC 
CTTGGTCATG TAACTCGAGT ATCCAGTCCC CAGGTGGAAG AAGGCGACAG CCGTTTTCTT 
GCGAATGAAG TCCTACAAGA GAACTACACT CATTTGCCTA AAGCAGATAT TTTCGCTCTC 
GCTCTGACTG TCGTCTGTGC TGCTGGGGCT GAACCACTTC CAACTAATGG GGACCAATGG 
CATGAAATCC GACAAGGAAA ACTACCTAGA CTACCACAAG TGCTTTCTCA AGAACTACTA 
GACTTACTAA AAGTTATGAT TAATCCTGAT CCAGAGAAAA GGCCTTCAGC TGTGGCACTA 
GTCAAGCATT CAGTGCTTCT CTCTGCTGCA AAAAAGAGCG CAGAGCAGCT CCGGATAGAA 
CTGAACGCTG AGAAATTCAA AAACTCGCTC TTGCAGAAAG AGCTGAAGAA GGCACAGATG 
GCGAAGGCTG CGGCCGAGGA GCGAGCGCTG TTCACTGACA GAATGACAAC GAGATCTACA 
ACACAAAACA GACCATCTCG ACTCATTGGG AAGAAAATGA ACCGCTCACT TAGTCTTACT 
ATCTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017665781.1
CDS	144..2072
Translation	MSFLSLQRAAPPRRVSARRAAAPIRQKLLFLSGHSDCEEEEEEEEEEGGSSSANSTGEDSAFQEADSPLSAARTPARSEPPLMEEEEEEMEPAAAPLPDEGDSWEEEGFGSSPVKSPGAYFLLDSPSPLAPHKGRRCCERSPPHPAAGGYRGPRGEEPGSPLPDYPGTPPHKTLRKLRLFDTPHTPKSLLSKAQGLGSSSVKLRGGSLFMNVGKSEKQEEDFRQTPHVNINPFTPDSMFLHNSEGKCRRRKRIHWNDSCGEDMEPSDGEPEEETMRPAKRITITESNMKSRYATEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAVLGQHSHVVRYYSAWAEDDHMLIQNEYCNGGSLADAISENYRNMRYFTEPELKDLLLQVARGLKYIHSMSLVHMDIKPSNIFISRTSVPSITLEEGDDDDWSSDRVIFKIGDLGHVTRVSSPQVEEGDSRFLANEVLQENYTHLPKADIFALALTVVCAAGAEPLPTNGDQWHEIRQGKLPRLPQVLSQELLDLLKVMINPDPEKRPSAVALVKHSVLLSAAKKSAEQLRIELNAEKFKNSLLQKELKKAQMAKAAAEERALFTDRMTTRSTTQNRPSRLIGKKMNRSLSLTIY

Target ORF information:

RefSeq Version	XM_017810292.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata WEE1 G2 checkpoint kinase (WEE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017810292.1

ORF Insert Sequence:

ATGAGCTTCC TCAGCCTGCA GCGGGCAGCG CCGCCCCGGC GCGTCTCGGC ACGGCGGGCG 
GCCGCCCCGA TCCGGCAGAA GCTGCTGTTC CTGAGCGGCC ACAGCGACTG CGAGGAGGAG 
GAGGAGGAAG AGGAGGAGGA AGGCGGCAGC AGCAGCGCCA ACAGCACCGG CGAGGACTCG 
GCGTTCCAGG AGGCGGACTC GCCGCTCTCG GCCGCGCGCA CCCCGGCGCG GAGCGAGCCG 
CCCCTGATGG AAGAGGAGGA GGAGGAGATG GAGCCGGCGG CGGCGCCGCT GCCGGACGAG 
GGGGACTCGT GGGAGGAGGA AGGGTTCGGC TCCTCGCCGG TCAAGTCGCC CGGAGCCTAT 
TTCCTGCTCG ACTCGCCCTC GCCGCTGGCC CCGCACAAGG GCCGCCGCTG CTGCGAGCGC 
TCCCCGCCGC ACCCGGCGGC CGGGGGATAC CGGGGGCCGC GAGGCGAGGA GCCCGGCTCG 
CCGCTGCCCG ACTACCCGGG CACGCCGCCG CACAAGACCC TGCGCAAGCT GCGCCTCTTC 
GACACGCCGC ACACGCCCAA GAGCTTGCTT TCTAAAGCAC AAGGACTAGG CTCCAGCTCA 
GTCAAACTTC GAGGGGGCTC ACTGTTTATG AATGTTGGGA AATCTGAGAA GCAAGAAGAA 
GATTTTAGAC AAACACCTCA TGTGAACATC AATCCCTTCA CTCCCGACTC CATGTTCCTT 
CACAACTCTG AAGGAAAATG TCGTAGGAGG AAGAGGATAC ACTGGAATGA CTCTTGTGGA 
GAGGACATGG AACCAAGTGA TGGAGAGCCT GAAGAGGAAA CCATGAGACC TGCTAAGAGG 
ATAACAATAA CAGAAAGTAA TATGAAGTCA CGGTATGCAA CTGAATTTCA TGAACTGGAG 
AAGATTGGAT CTGGTGAATT TGGTTCTGTG TTTAAATGTG TGAAGAGGTT AGATGGCTGC 
ATTTATGCAA TAAAGCGATC CAAGAAACCC TTGGCTGGCT CAGTGGATGA GCAGAATGCT 
TTAAGAGAGG TCTATGCACA TGCAGTTCTG GGACAGCATT CTCATGTAGT GAGGTACTAC 
TCTGCATGGG CAGAAGATGA TCATATGCTT ATACAGAATG AATATTGCAA TGGTGGCAGT 
TTAGCAGATG CCATAAGTGA AAATTACAGG AATATGCGTT ACTTTACTGA ACCAGAACTG 
AAGGACCTGC TACTTCAAGT GGCTCGAGGT TTAAAATACA TACATTCAAT GTCACTGGTA 
CACATGGATA TCAAACCTAG TAATATTTTC ATATCTAGGA CATCAGTCCC GAGTATAACT 
TTAGAAGAAG GTGATGATGA TGACTGGTCA TCTGATAGAG TCATATTTAA AATAGGTGAC 
CTTGGTCATG TAACTCGAGT ATCCAGTCCC CAGGTGGAAG AAGGCGACAG CCGTTTTCTT 
GCGAATGAAG TCCTACAAGA GAACTACACT CATTTGCCTA AAGCAGATAT TTTCGCTCTC 
GCTCTGACTG TCGTCTGTGC TGCTGGGGCT GAACCACTTC CAACTAATGG GGACCAATGG 
CATGAAATCC GACAAGGAAA ACTACCTAGA CTACCACAAG TGCTTTCTCA AGAACTACTA 
GACTTACTAA AAGTTATGAT TAATCCTGAT CCAGAGAAAA GGCCTTCAGC TGTGGCACTA 
GTCAAGCATT CAGTGCTTCT CTCTGCTGCA AAAAAGAGCG CAGAGCAGCT CCGGATAGAA 
CTGAACGCTG AGAAATTCAA AAACTCGCTC TTGCAGAAAG AGCTGAAGAA GGCACAGATG 
GCGAAGGCTG CGGCCGAGGA GCGAGCGCTG TTCACTGACA GAATGACAAC GAGATCTACA 
ACACAAAACA GACCATCTCG ACTCATTGGG AAGAAAATGA ACCGCTCACT TAGTCTTACT 
ATCTACTAA