LCMT2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	LCMT2
Entrez Gene ID	9836
Full Name	leucine carboxyl methyltransferase 2
Synonyms	PPM2,TYW4
General protein information	Preferred Names leucine carboxyl methyltransferase 2 Names tRNA wybutosine-synthesizing protein 4 p21WAF1/CIP1 promoter-interacting protein tRNA yW-synthesizing protein 4 tRNA(Phe) (7-(3-amino-3-(methoxycarbonyl)propyl)wyosine(37)-N)-methoxycarbonyltransferase tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase tRNA-Wybutosine-synthesizing protein 4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q15.3
Summary	The protein encoded by this intronless gene belongs to the highly variable methyltransferase superfamily. This gene is the inferred homolog of the Saccharomyces cerevisiae carboxymethyltransferase gene PPM2 that is essential for the synthesis of the hypermodified guanosine Wybutosine (yW). [provided by RefSeq, Jul 2008].
Disorder MIM:	611246

mRNA and Protein(s)

mRNA	Protein	Name
NM_014793.5	NP_055608.2	tRNA wybutosine-synthesizing protein 4
NM_014793.4	NP_055608.2	tRNA wybutosine-synthesizing protein 4

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LCMT2	XP_417263.3
Saccharomyces cerevisiae (baker's yeast)	PPM2	NP_014500.2
Magnaporthe oryzae (rice blast fungus)	MGG_03140	XP_003716857.1
Rattus norvegicus (Norway rat)	Lcmt2	NP_001011956.1
Homo sapiens (human)	LCMT2	NP_055608.2
Canis lupus familiaris (dog)	LCMT2	XP_003640071.1
Mus musculus (house mouse)	Lcmt2	NP_808514.2
Schizosaccharomyces pombe (fission yeast)	SPBC19C7.08c	NP_596164.1
Xenopus tropicalis (tropical clawed frog)	lcmt2	NP_001119971.1
	LCMT2	XP_001106399.1
Bos taurus (cattle)	LCMT2	XP_002696850.1
Neurospora crassa	NCU05392	XP_963480.2
Danio rerio (zebrafish)	lcmt2	NP_001122029.1
Eremothecium gossypii	AGOS_ADL194W	NP_983902.1

Gene Ontology
Bibliography

Related articles in PubMed

Widespread macromolecular interaction perturbations in human genetic disorders.
Sahni N, Yi S, Taipale M, Fuxman Bass JI, Coulombe-Huntington J, Yang F, Peng J, Weile J, Karras GI, Wang Y, Kov?cs IA, Kamburov A, Krykbaeva I, Lam MH, Tucker G, Khurana V, Sharma A, Liu YY, Yachie N, Zhong Q, Shen Y, Palagi A, San-Miguel A, Fan C, Balcha D, Dricot A, Jordan DM, Walsh JM, Shah AA, Yang X, Stoyanova AK, Leighton A, Calderwood MA, Jacob Y, Cusick ME, Salehi-Ashtiani K, Whitesell LJ, Sunyaev S, Berger B, Barab?si AL, Charloteaux B, Hill DE, Hao T, Roth FP, Xia Y, Walhout AJM, Lindquist S, Vidal M
Cell161(3)647-660(2015 Apr)

KR-POK interacts with p53 and represses its ability to activate transcription of p21WAF1/CDKN1A.
Jeon BN, Kim MK, Choi WI, Koh DI, Hong SY, Kim KS, Kim M, Yun CO, Yoon J, Choi KY, Lee KR, Nephew KP, Hur MW
Cancer research72(5)1137-48(2012 Mar)

Structural basis of tRNA modification with CO2 fixation and methylation by wybutosine synthesizing enzyme TYW4.
Suzuki Y, Noma A, Suzuki T, Ishitani R, Nureki O
Nucleic acids research37(9)2910-25(2009 May)

Independent and complementary methods for large-scale structural analysis of mammalian chromatin.
Dennis JH, Fan HY, Reynolds SM, Yuan G, Meldrim JC, Richter DJ, Peterson DG, Rando OJ, Noble WS, Kingston RE
Genome research17(6)928-39(2007 Jun)

Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA.
Noma A, Kirino Y, Ikeuchi Y, Suzuki T
The EMBO journal25(10)2142-54(2006 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Kr-pok interacted directly with the DNA binding and oligomerization domains of p53. This interaction was essential for Kr-pok to bind the distal promoter region of the CDKN1A gene, p21WAF1, and to inhibit p53-mediated transcriptional activation of CDKN1A.
Title: KR-POK interacts with p53 and represses its ability to activate transcription of p21WAF1/CDKN1A.

This paper reports crystal structures of a yeast homolog, TYW4.
Title: Structural basis of tRNA modification with CO2 fixation and methylation by wybutosine synthesizing enzyme TYW4.

In conclusion, we report that the transcription factor Sp1 involved in the p38MAPK-mediated control of p21WAF1 regulation on VSMC in response to BITC has now been identified.
Title: p38MAPK mediates benzyl isothiocyanate-induced p21WAF1 expression in vascular smooth muscle cells via the regulation of Sp1.

p21WAF1 and Bax may be used as the markers in the assessment of GIST malignant potential.
Title: Clinicopathological and immunohistochemical analysis of gastrointestinal stromal tumor.

Inacativaton of this cell cycle regulatory genes by DNA methylation could be associated with tumorigenesis in NK cell disorders.
Title: Methylation status analysis of cell cycle regulatory genes (p16INK4A, p15INK4B, p21Waf1/Cip1, p27Kip1 and p73) in natural killer cell disorders.

The following LCMT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LCMT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu08846	NM_014793.5 Latest version!	Homo sapiens leucine carboxyl methyltransferase 2 (LCMT2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
View Old Accession Versions >>
OHu08846	NM_014793.4	Homo sapiens leucine carboxyl methyltransferase 2 (LCMT2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LCMT2 ( NM_014793.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_055608.2 Homo sapiens leucine carboxyl methyltransferase 2 (LCMT2), mRNA.

Price & Availability↑

CloneID	OHu08846
Clone ID Related Accession (Same CDS sequence)	NM_014793.5 , NM_014793.4
Accession Version	NM_014793.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Homo sapiens(human)
Product	tRNA wybutosine-synthesizing protein 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC015949.2 and AC009852.9. On May 17, 2019 this sequence version replaced NM_014793.4. ##Evidence-Data-START## Transcript is intronless :: SRR1803613.31183.1, SRR1803616.44518.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000305641.7/ ENSP00000307214.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCCCCC GGAGCCGTGA GCGTCGGGCA GGCGCGGTAC AGAACACCAA CGACAGCAGC 
GCCCTCAGCA AGCGTTCCCT GGCCGCGCGC GGGTACGTGC AGGACCCCTT TGCCGCGTTG 
CTGGTTCCGG GCGCGGCGCG CCGCGCACCG CTCATTCACC GAGGCTACTA CGTCCGCGCA 
CGCGCCGTGA GGCACTGCGT GCGCGCTTTT TTGGAGCAGA TTGGCGCGCC CCAGGCCGCG 
CTTCGCGCGC AGATCTTGTC TCTCGGCGCT GGCTTCGACT CGCTCTATTT TCGCTTAAAA 
ACCGCGGGCC GCCTGGCCCG GGCTGCAGTC TGGGAGGTGG ATTTTCCGGA CGTGGCGCGG 
CGCAAAGCAG AAAGGATTGG AGAGACGCCA GAGCTGTGCG CGTTAACCGG GCCTTTCGAG 
AGGGGGGAGC CCGCGTCCGC GCTGTGCTTT GAGAGCGCAG ACTACTGCAT CCTGGGTCTG 
GACTTGCGGC AGCTCCAGCG AGTGGAGGAG GCCCTGGGCG CCGCGGGGCT CGACGCAGCC 
TCACCCACTC TGCTCCTGGC CGAGGCGGTG CTGACCTACC TCGAGCCGGA GAGTGCCGCG 
GCCCTCATCG CCTGGGCAGC CCAGCGTTTT CCTAATGCCC TTTTCGTGGT CTATGAGCAG 
ATGAGGCCTC AAGACGCCTT TGGCCAGTTC ATGCTGCAAC ATTTTCGGCA GCTAAACTCC 
CCCCTGCATG GCCTGGAGCG TTTTCCTGAC GTGGAGGCGC AGCGGCGCCG CTTCCTTCAA 
GCTGGCTGGA CCGCCTGCGG TGCCGTGGAC ATGAATGAAT TCTATCACTG CTTTCTTCCC 
GCAGAAGAAC GCCGGCGGGT GGAAAATATT GAACCCTTTG ACGAATTTGA GGAGTGGCAT 
CTGAAGTGCG CCCATTATTT CATTCTGGCA GCTTCTAGGG GAGACACCCT CTCCCACACC 
CTAGTGTTTC CATCCTCAGA GGCATTTCCT CGCGTAAATC CTGCTTCGCC TTCAGGGGTA 
TTCCCTGCCA GCGTAGTCAG TAGCGAGGGC CAGGTCCCAA ACCTGAAGAG ATATGGCCAC 
GCCTCTGTCT TCTTGAGCCC AGACGTTATT CTCAGTGCAG GAGGATTTGG AGAGCAGGAG 
GGGCGGCACT GCCGAGTGAG CCAGTTTCAC TTGCTCTCAA GAGATTGTGA CTCTGAATGG 
AAAGGCAGCC AAATAGGCAG TTGTGGGACT GGAGTTCAGT GGGATGGACG CCTTTATCAC 
ACCATGACAA GACTCTCAGA GAGTCGGGTT CTGGTTCTGG GAGGGAGACT GTCCCCAGTA 
AGTCCAGCCT TGGGGGTTCT CCAGCTTCAT TTTTTTAAGA GTGAGGATAA TAACACTGAG 
GACCTGAAAG TGACAATAAC AAAGGCTGGC CGAAAGGATG ATTCCACTTT GTGTTGTTGG 
CGGCATTCAA CAACAGAAGT GTCCTGTCAG AATCAGGAAT ATTTGTTTGT GTATGGGGGT 
CGAAGCGTGG TGGAACCTGT ACTAAGTGAC TGGCATTTCC TCCATGTAGG GACAATGGCT 
TGGGTCAGGA TCCCAGTGGA GGGAGAAGTA CCTGAAGCCC GGCATTCTCA CAGTGCCTGC 
ACTTGGCAAG GGGGAGCCCT TATTGCTGGA GGTCTCGGGG CTTCTGAGGA GCCATTGAAC 
TCTGTGCTCT TTCTGAGACC AATCTCTTGT GGATTCCTCT GGGAGTCAGT AGACATCCAG 
CCTCCCATTA CCCCAAGGTA CTCCCACACA GCTCATGTGC TCAATGGAAA GCTGTTACTG 
GTTGGAGGGA TCTGGATTCA TTCCTCCTCA TTTCCTGGAG TGACTGTGAT CAATTTGACT 
ACAGGATTGA GCTCTGAGTA TCAGATTGAC ACAACATATG TGCCATGGCC ATTAATGTTA 
CACAACCATA CTAGTATCCT TCTTCCTGAA GAGCAACAGC TCCTGCTCCT TGGAGGTGGT 
GGGAACTGCT TTTCCTTTGG TACCTACTTC AACCCCCATA CAGTCACATT AGACCTTTCT 
TCCTTAAGTG CTGGGCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055608.2
CDS	85..2145
Translation	MGPRSRERRAGAVQNTNDSSALSKRSLAARGYVQDPFAALLVPGAARRAPLIHRGYYVRARAVRHCVRAFLEQIGAPQAALRAQILSLGAGFDSLYFRLKTAGRLARAAVWEVDFPDVARRKAERIGETPELCALTGPFERGEPASALCFESADYCILGLDLRQLQRVEEALGAAGLDAASPTLLLAEAVLTYLEPESAAALIAWAAQRFPNALFVVYEQMRPQDAFGQFMLQHFRQLNSPLHGLERFPDVEAQRRRFLQAGWTACGAVDMNEFYHCFLPAEERRRVENIEPFDEFEEWHLKCAHYFILAASRGDTLSHTLVFPSSEAFPRVNPASPSGVFPASVVSSEGQVPNLKRYGHASVFLSPDVILSAGGFGEQEGRHCRVSQFHLLSRDCDSEWKGSQIGSCGTGVQWDGRLYHTMTRLSESRVLVLGGRLSPVSPALGVLQLHFFKSEDNNTEDLKVTITKAGRKDDSTLCCWRHSTTEVSCQNQEYLFVYGGRSVVEPVLSDWHFLHVGTMAWVRIPVEGEVPEARHSHSACTWQGGALIAGGLGASEEPLNSVLFLRPISCGFLWESVDIQPPITPRYSHTAHVLNGKLLLVGGIWIHSSSFPGVTVINLTTGLSSEYQIDTTYVPWPLMLHNHTSILLPEEQQLLLLGGGGNCFSFGTYFNPHTVTLDLSSLSAGQ

Target ORF information:

RefSeq Version	NM_014793.5
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine carboxyl methyltransferase 2 (LCMT2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014793.5

ORF Insert Sequence:

ATGGGCCCCC GGAGCCGTGA GCGTCGGGCA GGCGCGGTAC AGAACACCAA CGACAGCAGC 
GCCCTCAGCA AGCGTTCCCT GGCCGCGCGC GGGTACGTGC AGGACCCCTT TGCCGCGTTG 
CTGGTTCCGG GCGCGGCGCG CCGCGCACCG CTCATTCACC GAGGCTACTA CGTCCGCGCA 
CGCGCCGTGA GGCACTGCGT GCGCGCTTTT TTGGAGCAGA TTGGCGCGCC CCAGGCCGCG 
CTTCGCGCGC AGATCTTGTC TCTCGGCGCT GGCTTCGACT CGCTCTATTT TCGCTTAAAA 
ACCGCGGGCC GCCTGGCCCG GGCTGCAGTC TGGGAGGTGG ATTTTCCGGA CGTGGCGCGG 
CGCAAAGCAG AAAGGATTGG AGAGACGCCA GAGCTGTGCG CGTTAACCGG GCCTTTCGAG 
AGGGGGGAGC CCGCGTCCGC GCTGTGCTTT GAGAGCGCAG ACTACTGCAT CCTGGGTCTG 
GACTTGCGGC AGCTCCAGCG AGTGGAGGAG GCCCTGGGCG CCGCGGGGCT CGACGCAGCC 
TCACCCACTC TGCTCCTGGC CGAGGCGGTG CTGACCTACC TCGAGCCGGA GAGTGCCGCG 
GCCCTCATCG CCTGGGCAGC CCAGCGTTTT CCTAATGCCC TTTTCGTGGT CTATGAGCAG 
ATGAGGCCTC AAGACGCCTT TGGCCAGTTC ATGCTGCAAC ATTTTCGGCA GCTAAACTCC 
CCCCTGCATG GCCTGGAGCG TTTTCCTGAC GTGGAGGCGC AGCGGCGCCG CTTCCTTCAA 
GCTGGCTGGA CCGCCTGCGG TGCCGTGGAC ATGAATGAAT TCTATCACTG CTTTCTTCCC 
GCAGAAGAAC GCCGGCGGGT GGAAAATATT GAACCCTTTG ACGAATTTGA GGAGTGGCAT 
CTGAAGTGCG CCCATTATTT CATTCTGGCA GCTTCTAGGG GAGACACCCT CTCCCACACC 
CTAGTGTTTC CATCCTCAGA GGCATTTCCT CGCGTAAATC CTGCTTCGCC TTCAGGGGTA 
TTCCCTGCCA GCGTAGTCAG TAGCGAGGGC CAGGTCCCAA ACCTGAAGAG ATATGGCCAC 
GCCTCTGTCT TCTTGAGCCC AGACGTTATT CTCAGTGCAG GAGGATTTGG AGAGCAGGAG 
GGGCGGCACT GCCGAGTGAG CCAGTTTCAC TTGCTCTCAA GAGATTGTGA CTCTGAATGG 
AAAGGCAGCC AAATAGGCAG TTGTGGGACT GGAGTTCAGT GGGATGGACG CCTTTATCAC 
ACCATGACAA GACTCTCAGA GAGTCGGGTT CTGGTTCTGG GAGGGAGACT GTCCCCAGTA 
AGTCCAGCCT TGGGGGTTCT CCAGCTTCAT TTTTTTAAGA GTGAGGATAA TAACACTGAG 
GACCTGAAAG TGACAATAAC AAAGGCTGGC CGAAAGGATG ATTCCACTTT GTGTTGTTGG 
CGGCATTCAA CAACAGAAGT GTCCTGTCAG AATCAGGAAT ATTTGTTTGT GTATGGGGGT 
CGAAGCGTGG TGGAACCTGT ACTAAGTGAC TGGCATTTCC TCCATGTAGG GACAATGGCT 
TGGGTCAGGA TCCCAGTGGA GGGAGAAGTA CCTGAAGCCC GGCATTCTCA CAGTGCCTGC 
ACTTGGCAAG GGGGAGCCCT TATTGCTGGA GGTCTCGGGG CTTCTGAGGA GCCATTGAAC 
TCTGTGCTCT TTCTGAGACC AATCTCTTGT GGATTCCTCT GGGAGTCAGT AGACATCCAG 
CCTCCCATTA CCCCAAGGTA CTCCCACACA GCTCATGTGC TCAATGGAAA GCTGTTACTG 
GTTGGAGGGA TCTGGATTCA TTCCTCCTCA TTTCCTGGAG TGACTGTGAT CAATTTGACT 
ACAGGATTGA GCTCTGAGTA TCAGATTGAC ACAACATATG TGCCATGGCC ATTAATGTTA 
CACAACCATA CTAGTATCCT TCTTCCTGAA GAGCAACAGC TCCTGCTCCT TGGAGGTGGT 
GGGAACTGCT TTTCCTTTGG TACCTACTTC AACCCCCATA CAGTCACATT AGACCTTTCT 
TCCTTAAGTG CTGGGCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LCMT2 ( NM_014793.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055608.2 Homo sapiens leucine carboxyl methyltransferase 2 (LCMT2), mRNA.

Price & Availability↑

CloneID	OHu08846
Clone ID Related Accession (Same CDS sequence)	NM_014793.5 , NM_014793.4
Accession Version	NM_014793.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	tRNA wybutosine-synthesizing protein 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB260484.1 and BC015949.2. On Dec 8, 2010 this sequence version replaced NM_014793.3. ##Evidence-Data-START## Transcript is intronless :: BC015949.2 [ECO:0000345] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCCCCC GGAGCCGTGA GCGTCGGGCA GGCGCGGTAC AGAACACCAA CGACAGCAGC 
GCCCTCAGCA AGCGTTCCCT GGCCGCGCGC GGGTACGTGC AGGACCCCTT TGCCGCGTTG 
CTGGTTCCGG GCGCGGCGCG CCGCGCACCG CTCATTCACC GAGGCTACTA CGTCCGCGCA 
CGCGCCGTGA GGCACTGCGT GCGCGCTTTT TTGGAGCAGA TTGGCGCGCC CCAGGCCGCG 
CTTCGCGCGC AGATCTTGTC TCTCGGCGCT GGCTTCGACT CGCTCTATTT TCGCTTAAAA 
ACCGCGGGCC GCCTGGCCCG GGCTGCAGTC TGGGAGGTGG ATTTTCCGGA CGTGGCGCGG 
CGCAAAGCAG AAAGGATTGG AGAGACGCCA GAGCTGTGCG CGTTAACCGG GCCTTTCGAG 
AGGGGGGAGC CCGCGTCCGC GCTGTGCTTT GAGAGCGCAG ACTACTGCAT CCTGGGTCTG 
GACTTGCGGC AGCTCCAGCG AGTGGAGGAG GCCCTGGGCG CCGCGGGGCT CGACGCAGCC 
TCACCCACTC TGCTCCTGGC CGAGGCGGTG CTGACCTACC TCGAGCCGGA GAGTGCCGCG 
GCCCTCATCG CCTGGGCAGC CCAGCGTTTT CCTAATGCCC TTTTCGTGGT CTATGAGCAG 
ATGAGGCCTC AAGACGCCTT TGGCCAGTTC ATGCTGCAAC ATTTTCGGCA GCTAAACTCC 
CCCCTGCATG GCCTGGAGCG TTTTCCTGAC GTGGAGGCGC AGCGGCGCCG CTTCCTTCAA 
GCTGGCTGGA CCGCCTGCGG TGCCGTGGAC ATGAATGAAT TCTATCACTG CTTTCTTCCC 
GCAGAAGAAC GCCGGCGGGT GGAAAATATT GAACCCTTTG ACGAATTTGA GGAGTGGCAT 
CTGAAGTGCG CCCATTATTT CATTCTGGCA GCTTCTAGGG GAGACACCCT CTCCCACACC 
CTAGTGTTTC CATCCTCAGA GGCATTTCCT CGCGTAAATC CTGCTTCGCC TTCAGGGGTA 
TTCCCTGCCA GCGTAGTCAG TAGCGAGGGC CAGGTCCCAA ACCTGAAGAG ATATGGCCAC 
GCCTCTGTCT TCTTGAGCCC AGACGTTATT CTCAGTGCAG GAGGATTTGG AGAGCAGGAG 
GGGCGGCACT GCCGAGTGAG CCAGTTTCAC TTGCTCTCAA GAGATTGTGA CTCTGAATGG 
AAAGGCAGCC AAATAGGCAG TTGTGGGACT GGAGTTCAGT GGGATGGACG CCTTTATCAC 
ACCATGACAA GACTCTCAGA GAGTCGGGTT CTGGTTCTGG GAGGGAGACT GTCCCCAGTA 
AGTCCAGCCT TGGGGGTTCT CCAGCTTCAT TTTTTTAAGA GTGAGGATAA TAACACTGAG 
GACCTGAAAG TGACAATAAC AAAGGCTGGC CGAAAGGATG ATTCCACTTT GTGTTGTTGG 
CGGCATTCAA CAACAGAAGT GTCCTGTCAG AATCAGGAAT ATTTGTTTGT GTATGGGGGT 
CGAAGCGTGG TGGAACCTGT ACTAAGTGAC TGGCATTTCC TCCATGTAGG GACAATGGCT 
TGGGTCAGGA TCCCAGTGGA GGGAGAAGTA CCTGAAGCCC GGCATTCTCA CAGTGCCTGC 
ACTTGGCAAG GGGGAGCCCT TATTGCTGGA GGTCTCGGGG CTTCTGAGGA GCCATTGAAC 
TCTGTGCTCT TTCTGAGACC AATCTCTTGT GGATTCCTCT GGGAGTCAGT AGACATCCAG 
CCTCCCATTA CCCCAAGGTA CTCCCACACA GCTCATGTGC TCAATGGAAA GCTGTTACTG 
GTTGGAGGGA TCTGGATTCA TTCCTCCTCA TTTCCTGGAG TGACTGTGAT CAATTTGACT 
ACAGGATTGA GCTCTGAGTA TCAGATTGAC ACAACATATG TGCCATGGCC ATTAATGTTA 
CACAACCATA CTAGTATCCT TCTTCCTGAA GAGCAACAGC TCCTGCTCCT TGGAGGTGGT 
GGGAACTGCT TTTCCTTTGG TACCTACTTC AACCCCCATA CAGTCACATT AGACCTTTCT 
TCCTTAAGTG CTGGGCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055608.2
CDS	134..2194
Translation	MGPRSRERRAGAVQNTNDSSALSKRSLAARGYVQDPFAALLVPGAARRAPLIHRGYYVRARAVRHCVRAFLEQIGAPQAALRAQILSLGAGFDSLYFRLKTAGRLARAAVWEVDFPDVARRKAERIGETPELCALTGPFERGEPASALCFESADYCILGLDLRQLQRVEEALGAAGLDAASPTLLLAEAVLTYLEPESAAALIAWAAQRFPNALFVVYEQMRPQDAFGQFMLQHFRQLNSPLHGLERFPDVEAQRRRFLQAGWTACGAVDMNEFYHCFLPAEERRRVENIEPFDEFEEWHLKCAHYFILAASRGDTLSHTLVFPSSEAFPRVNPASPSGVFPASVVSSEGQVPNLKRYGHASVFLSPDVILSAGGFGEQEGRHCRVSQFHLLSRDCDSEWKGSQIGSCGTGVQWDGRLYHTMTRLSESRVLVLGGRLSPVSPALGVLQLHFFKSEDNNTEDLKVTITKAGRKDDSTLCCWRHSTTEVSCQNQEYLFVYGGRSVVEPVLSDWHFLHVGTMAWVRIPVEGEVPEARHSHSACTWQGGALIAGGLGASEEPLNSVLFLRPISCGFLWESVDIQPPITPRYSHTAHVLNGKLLLVGGIWIHSSSFPGVTVINLTTGLSSEYQIDTTYVPWPLMLHNHTSILLPEEQQLLLLGGGGNCFSFGTYFNPHTVTLDLSSLSAGQ

Target ORF information:

RefSeq Version	NM_014793.4
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine carboxyl methyltransferase 2 (LCMT2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014793.4

ORF Insert Sequence:

ATGGGCCCCC GGAGCCGTGA GCGTCGGGCA GGCGCGGTAC AGAACACCAA CGACAGCAGC 
GCCCTCAGCA AGCGTTCCCT GGCCGCGCGC GGGTACGTGC AGGACCCCTT TGCCGCGTTG 
CTGGTTCCGG GCGCGGCGCG CCGCGCACCG CTCATTCACC GAGGCTACTA CGTCCGCGCA 
CGCGCCGTGA GGCACTGCGT GCGCGCTTTT TTGGAGCAGA TTGGCGCGCC CCAGGCCGCG 
CTTCGCGCGC AGATCTTGTC TCTCGGCGCT GGCTTCGACT CGCTCTATTT TCGCTTAAAA 
ACCGCGGGCC GCCTGGCCCG GGCTGCAGTC TGGGAGGTGG ATTTTCCGGA CGTGGCGCGG 
CGCAAAGCAG AAAGGATTGG AGAGACGCCA GAGCTGTGCG CGTTAACCGG GCCTTTCGAG 
AGGGGGGAGC CCGCGTCCGC GCTGTGCTTT GAGAGCGCAG ACTACTGCAT CCTGGGTCTG 
GACTTGCGGC AGCTCCAGCG AGTGGAGGAG GCCCTGGGCG CCGCGGGGCT CGACGCAGCC 
TCACCCACTC TGCTCCTGGC CGAGGCGGTG CTGACCTACC TCGAGCCGGA GAGTGCCGCG 
GCCCTCATCG CCTGGGCAGC CCAGCGTTTT CCTAATGCCC TTTTCGTGGT CTATGAGCAG 
ATGAGGCCTC AAGACGCCTT TGGCCAGTTC ATGCTGCAAC ATTTTCGGCA GCTAAACTCC 
CCCCTGCATG GCCTGGAGCG TTTTCCTGAC GTGGAGGCGC AGCGGCGCCG CTTCCTTCAA 
GCTGGCTGGA CCGCCTGCGG TGCCGTGGAC ATGAATGAAT TCTATCACTG CTTTCTTCCC 
GCAGAAGAAC GCCGGCGGGT GGAAAATATT GAACCCTTTG ACGAATTTGA GGAGTGGCAT 
CTGAAGTGCG CCCATTATTT CATTCTGGCA GCTTCTAGGG GAGACACCCT CTCCCACACC 
CTAGTGTTTC CATCCTCAGA GGCATTTCCT CGCGTAAATC CTGCTTCGCC TTCAGGGGTA 
TTCCCTGCCA GCGTAGTCAG TAGCGAGGGC CAGGTCCCAA ACCTGAAGAG ATATGGCCAC 
GCCTCTGTCT TCTTGAGCCC AGACGTTATT CTCAGTGCAG GAGGATTTGG AGAGCAGGAG 
GGGCGGCACT GCCGAGTGAG CCAGTTTCAC TTGCTCTCAA GAGATTGTGA CTCTGAATGG 
AAAGGCAGCC AAATAGGCAG TTGTGGGACT GGAGTTCAGT GGGATGGACG CCTTTATCAC 
ACCATGACAA GACTCTCAGA GAGTCGGGTT CTGGTTCTGG GAGGGAGACT GTCCCCAGTA 
AGTCCAGCCT TGGGGGTTCT CCAGCTTCAT TTTTTTAAGA GTGAGGATAA TAACACTGAG 
GACCTGAAAG TGACAATAAC AAAGGCTGGC CGAAAGGATG ATTCCACTTT GTGTTGTTGG 
CGGCATTCAA CAACAGAAGT GTCCTGTCAG AATCAGGAAT ATTTGTTTGT GTATGGGGGT 
CGAAGCGTGG TGGAACCTGT ACTAAGTGAC TGGCATTTCC TCCATGTAGG GACAATGGCT 
TGGGTCAGGA TCCCAGTGGA GGGAGAAGTA CCTGAAGCCC GGCATTCTCA CAGTGCCTGC 
ACTTGGCAAG GGGGAGCCCT TATTGCTGGA GGTCTCGGGG CTTCTGAGGA GCCATTGAAC 
TCTGTGCTCT TTCTGAGACC AATCTCTTGT GGATTCCTCT GGGAGTCAGT AGACATCCAG 
CCTCCCATTA CCCCAAGGTA CTCCCACACA GCTCATGTGC TCAATGGAAA GCTGTTACTG 
GTTGGAGGGA TCTGGATTCA TTCCTCCTCA TTTCCTGGAG TGACTGTGAT CAATTTGACT 
ACAGGATTGA GCTCTGAGTA TCAGATTGAC ACAACATATG TGCCATGGCC ATTAATGTTA 
CACAACCATA CTAGTATCCT TCTTCCTGAA GAGCAACAGC TCCTGCTCCT TGGAGGTGGT 
GGGAACTGCT TTTCCTTTGG TACCTACTTC AACCCCCATA CAGTCACATT AGACCTTTCT 
TCCTTAAGTG CTGGGCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Widespread macromolecular interaction perturbations in human genetic disorders.
Cell161(3)647-660(2015 Apr)
Sahni N,Yi S,Taipale M,Fuxman Bass JI,Coulombe-Huntington J,Yang F,Peng J,Weile J,Karras GI,Wang Y,Kov?cs IA,Kamburov A,Krykbaeva I,Lam MH,Tucker G,Khurana V,Sharma A,Liu YY,Yachie N,Zhong Q,Shen Y,Palagi A,San-Miguel A,Fan C,Balcha D,Dricot A,Jordan DM,Walsh JM,Shah AA,Yang X,Stoyanova AK,Leighton A,Calderwood MA,Jacob Y,Cusick ME,Salehi-Ashtiani K,Whitesell LJ,Sunyaev S,Berger B,Barab?si AL,Charloteaux B,Hill DE,Hao T,Roth FP,Xia Y,Walhout AJM,Lindquist S,Vidal M

KR-POK interacts with p53 and represses its ability to activate transcription of p21WAF1/CDKN1A.
Cancer research72(5)1137-48(2012 Mar)
Jeon BN,Kim MK,Choi WI,Koh DI,Hong SY,Kim KS,Kim M,Yun CO,Yoon J,Choi KY,Lee KR,Nephew KP,Hur MW

Structural basis of tRNA modification with CO2 fixation and methylation by wybutosine synthesizing enzyme TYW4.
Nucleic acids research37(9)2910-25(2009 May)
Suzuki Y,Noma A,Suzuki T,Ishitani R,Nureki O

Independent and complementary methods for large-scale structural analysis of mammalian chromatin.
Genome research17(6)928-39(2007 Jun)
Dennis JH,Fan HY,Reynolds SM,Yuan G,Meldrim JC,Richter DJ,Peterson DG,Rando OJ,Noble WS,Kingston RE

Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA.
The EMBO journal25(10)2142-54(2006 May)
Noma A,Kirino Y,Ikeuchi Y,Suzuki T

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LCMT2 cDNA ORF clone, Homo sapiens(human)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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