NCU05392 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU05392
Entrez Gene ID	3879634
Full Name	leucine carboxyl methyltransferase 2
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011395268.1	XP_011393570.1	leucine carboxyl methyltransferase 2
XM_011395269.1	XP_011393571.1	leucine carboxyl methyltransferase 2, variant

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LCMT2	XP_417263.3
Saccharomyces cerevisiae (baker's yeast)	PPM2	NP_014500.2
Magnaporthe oryzae (rice blast fungus)	MGG_03140	XP_003716857.1
Rattus norvegicus (Norway rat)	Lcmt2	NP_001011956.1
Homo sapiens (human)	LCMT2	NP_055608.2
Canis lupus familiaris (dog)	LCMT2	XP_003640071.1
Mus musculus (house mouse)	Lcmt2	NP_808514.2
Schizosaccharomyces pombe (fission yeast)	SPBC19C7.08c	NP_596164.1
Xenopus tropicalis (tropical clawed frog)	lcmt2	NP_001119971.1
	LCMT2	XP_001106399.1
Bos taurus (cattle)	LCMT2	XP_002696850.1
Neurospora crassa	NCU05392	XP_963480.2
Danio rerio (zebrafish)	lcmt2	NP_001122029.1
Eremothecium gossypii	AGOS_ADL194W	NP_983902.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU05392 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU05392 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf12467	XM_011395269.1 Latest version!	Neurospora crassa OR74A leucine carboxyl methyltransferase 2, variant (NCU05392), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ONf12466	XM_011395268.1 Latest version!	Neurospora crassa OR74A leucine carboxyl methyltransferase 2 (NCU05392), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU05392 ( XM_011395269.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393571.1 Neurospora crassa OR74A leucine carboxyl methyltransferase 2, variant (NCU05392), partial mRNA.

Price & Availability↑

CloneID	ONf12467
Clone ID Related Accession (Same CDS sequence)	XM_011395269.1
Accession Version	XM_011395269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	leucine carboxyl methyltransferase 2, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026502). COMPLETENESS: incomplete on the 5' end.

ATGGAGACCA CAGAGGAGGT AGTAGCTCCA GCTACGACTC AGCAACCGGC TACCGAAGTC 
GGCTCAGCCA AGCCTACTAC TTCAACATAC AGCAAAAAGC AAAAGAAGCC AAAGGCGACG 
ACGACGACGA CGACGACAGA CGGGACCACG CCAACTCACA ATGAACACGC CAGCGCCAAA 
GACCCGAGAA AAGCTCAGGA TGATCAGGTT ATGGGCACAA ACAACTCGTC CATAGTCTCC 
AAACGCTCCG TCGAAAAACT CTACTACCCC AACGAGCCTC ATTTCTTCCG CTTCTTCGTC 
AAGAAATTCC AGCGACGCGC GCCGCTGATT AATCGCGGTT ATCACTTTCG TTTGCACGTC 
ATCGATGTGC TTGTTCGCAA CTTCTTGCAA GAGCAAAGAA CTGGAGACGC AAAGGGAAAA 
AGAAAAGTGG TGGTTAATTT GGGTTGTGGA AGCGATGTGC TGCCTTGGCA GTGTTTGGCT 
AGGTATCCGG ATGCTTGTCG TTCTGGTGAG AAAGACGGGG CGAAGTTTGT GGATGTCGAT 
TTCCCGGATC TGATTGAGCG GAAGAAGCGG ACGGTTTTGG AGACGCCGGA ACTTTTGGGG 
CCGTTGACGA ACGTGGTGGT ACCAGAGTTT GCTCCTGTGC CTAGTACTCC TGCGGCCACT 
ACTACGGCTG CGGCTACAAC TACCACAACG ACGGAACTCA AAACCACGGC AGCAACCGCT 
TCAAGCACCT CGACCGAAGC CCCTCAAAAG CCCAAGAAAT CCCCCAAACC AAAGGACAAA 
TCCAAAGCCG CCCGCGCCCC GGCCCCCACC ACCGCCCCTA CGGGTATCGT TTTCACCAGC 
GACCAATACG TTCAAATAGG CTGCGACCTG CGTGACCTAG CCACCCTTCA AGACTCCCTC 
ACCAGAGCCG TCGGTGGCGA TCTCTCCTCC TGCACCTTCC TCTTCGTTGC CGAGGTCTCC 
ATCACCTACA TGGAGACACC GGGAGCCGAC GCCGTCATAC AATGGGCTTC CTCGCTAGGC 
GACTCCGAGT TTGTCCTTCT CGAGCAACTG CTACCTTCGG GGCCGACCCA CCCGTTTGCC 
TCCACAATGC TATCGCATTT TCATAAACTC AACACCCCAA TCAAGAGCGT GGACGTGTAC 
CCTACGGTAG CCTCGCAGGT GGAGCGTTTC CGCTCTCGGG GCTGGGGCAG CGGTGATGTG 
CGCGTCTGGA CGCTGTGGGA GGCGTGGGCA GATGCCGAGG ATACTTTCGT CAATGCGGCG 
GAGAGACGCA GGCTGGACGA GGTGGAGCCG TTCGATGAGT GGGAGGAGTT TGCGCTTTTC 
GCGAGCCATT ATTGTGTGGT GAGGGCCAGG ACAGTGGCTA GGGACGGACA GAATAAGAAG 
AAAGAGAGGG GGATCCCGAA CGGAAGGGAG TTGGGGTTGC CGGTTGAGAA GGTCAAGGTG 
AGGTGGGATG ATGTCCCGGG GCAGAGAGGT CAGAGGAGGT TTGCGGCTGG GGCGGTGTTG 
ACTTCTTCTT CTCCGAGTTC GGAAAAGAAA GAGGAGGTGA AACTGCTGAA CGTTATGGGG 
CTGGGGACGA AGAGCCGGTT GCAGAACTGC GATGTTTACG GACGGAAGAA AGTGGGAGAT 
GCCGACGAGG CTGATGGTAC TGACGAAGAA AAGACGGCGG TGCCGTTCAC TTTCCGTGAG 
GGCGGCCCTT CAACGAGGAT GTGTCACTCG CTGACAGATT TCGGCACTGC CCAACTCCTC 
GTGGGAGGAC GGGCATCACC GTCCACGCCC CTCAAAGACT GCTGGCTGTT GGAGAAGACC 
AACGGCAGCG AGAGTGAATG GGCATGGAAA CGCACCAATG ATCTCCCGAT TCCGCTCTAC 
AGACATTCTG TCACGAGATT GGGGAAGACA GACATGGCGC TGCTTGCAGG TGGGAGGGGA 
GTTGCCGATA TCTTCCCTGA CTGGCTTCTA TATGAGCCCA AACTTGGATG GATCAGATGC 
GAGATTGCCG GTGACGTCAA GCCGACTTCG GTGTATGGCG CTACGCTTGC TTGCCTCCGT 
CAAGAGAGTG ACTCGTTCTC TGGTGTGTTT ACTGGCGGCT TGAGTGATGA TGGCCTCATT 
GCTGATCAGC TTCTCGCATG GAACCTGGAC GTATCCAACA GCAGCAGGCC GGTCGTCACT 
TTTGTTCCGC TACAGGTAAA GTCTGGTGAT GATGGCCGCG AAGAGGCTCT CTCACGTCTA 
CTTCTCACGA GGTTTGGCGC TTCTTGCCTG CCCCAAAGTC GGACCGACTT CTTGGTCTTT 
GGTGGTGTTA TCAAGGACCA CCTTTTGGAT ATGGAAGACG AGATCCTGCT ATGCTCACTC 
AAGGGCGACG GGGAACTCAC AATCACGCGA CGACTCGTTC CCGAGGCCGC TAATGCTAAG 
TCACCATCGC ACCCAGGCCC TTTGTTGTTG GTGGGAACTT CTCCAGTTGT CACGCCGGAC 
GACGGCAGCT TGATGATCGT GGGCGGCGGA GCAACATGCT TCTCCATGGG AACCTTCTGG 
AACAAGGGTA TTTCGACTCT AGAACTGGCA CTGCCAGCTG CGGTGGAGAA TGAAGCTTCA 
CCCATCCCCG CATATGGATG GGTGCACGAG AAAACAGTCG ACATTATCCC TGGTGAACCA 
AGGAGTCTTC CTCTCCGTAA TCAGGCACCC AACGGCGCTG AGGGGAACGC CAATGGCAGC 
GATCGTACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393571.1
CDS	1..2712
Translation	METTEEVVAPATTQQPATEVGSAKPTTSTYSKKQKKPKATTTTTTTDGTTPTHNEHASAKDPRKAQDDQVMGTNNSSIVSKRSVEKLYYPNEPHFFRFFVKKFQRRAPLINRGYHFRLHVIDVLVRNFLQEQRTGDAKGKRKVVVNLGCGSDVLPWQCLARYPDACRSGEKDGAKFVDVDFPDLIERKKRTVLETPELLGPLTNVVVPEFAPVPSTPAATTTAAATTTTTTELKTTAATASSTSTEAPQKPKKSPKPKDKSKAARAPAPTTAPTGIVFTSDQYVQIGCDLRDLATLQDSLTRAVGGDLSSCTFLFVAEVSITYMETPGADAVIQWASSLGDSEFVLLEQLLPSGPTHPFASTMLSHFHKLNTPIKSVDVYPTVASQVERFRSRGWGSGDVRVWTLWEAWADAEDTFVNAAERRRLDEVEPFDEWEEFALFASHYCVVRARTVARDGQNKKKERGIPNGRELGLPVEKVKVRWDDVPGQRGQRRFAAGAVLTSSSPSSEKKEEVKLLNVMGLGTKSRLQNCDVYGRKKVGDADEADGTDEEKTAVPFTFREGGPSTRMCHSLTDFGTAQLLVGGRASPSTPLKDCWLLEKTNGSESEWAWKRTNDLPIPLYRHSVTRLGKTDMALLAGGRGVADIFPDWLLYEPKLGWIRCEIAGDVKPTSVYGATLACLRQESDSFSGVFTGGLSDDGLIADQLLAWNLDVSNSSRPVVTFVPLQVKSGDDGREEALSRLLLTRFGASCLPQSRTDFLVFGGVIKDHLLDMEDEILLCSLKGDGELTITRRLVPEAANAKSPSHPGPLLLVGTSPVVTPDDGSLMIVGGGATCFSMGTFWNKGISTLELALPAAVENEASPIPAYGWVHEKTVDIIPGEPRSLPLRNQAPNGAEGNANGSDRT

Target ORF information:

RefSeq Version	XM_011395269.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A leucine carboxyl methyltransferase 2, variant (NCU05392), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395269.1

ORF Insert Sequence:

ATGGAGACCA CAGAGGAGGT AGTAGCTCCA GCTACGACTC AGCAACCGGC TACCGAAGTC 
GGCTCAGCCA AGCCTACTAC TTCAACATAC AGCAAAAAGC AAAAGAAGCC AAAGGCGACG 
ACGACGACGA CGACGACAGA CGGGACCACG CCAACTCACA ATGAACACGC CAGCGCCAAA 
GACCCGAGAA AAGCTCAGGA TGATCAGGTT ATGGGCACAA ACAACTCGTC CATAGTCTCC 
AAACGCTCCG TCGAAAAACT CTACTACCCC AACGAGCCTC ATTTCTTCCG CTTCTTCGTC 
AAGAAATTCC AGCGACGCGC GCCGCTGATT AATCGCGGTT ATCACTTTCG TTTGCACGTC 
ATCGATGTGC TTGTTCGCAA CTTCTTGCAA GAGCAAAGAA CTGGAGACGC AAAGGGAAAA 
AGAAAAGTGG TGGTTAATTT GGGTTGTGGA AGCGATGTGC TGCCTTGGCA GTGTTTGGCT 
AGGTATCCGG ATGCTTGTCG TTCTGGTGAG AAAGACGGGG CGAAGTTTGT GGATGTCGAT 
TTCCCGGATC TGATTGAGCG GAAGAAGCGG ACGGTTTTGG AGACGCCGGA ACTTTTGGGG 
CCGTTGACGA ACGTGGTGGT ACCAGAGTTT GCTCCTGTGC CTAGTACTCC TGCGGCCACT 
ACTACGGCTG CGGCTACAAC TACCACAACG ACGGAACTCA AAACCACGGC AGCAACCGCT 
TCAAGCACCT CGACCGAAGC CCCTCAAAAG CCCAAGAAAT CCCCCAAACC AAAGGACAAA 
TCCAAAGCCG CCCGCGCCCC GGCCCCCACC ACCGCCCCTA CGGGTATCGT TTTCACCAGC 
GACCAATACG TTCAAATAGG CTGCGACCTG CGTGACCTAG CCACCCTTCA AGACTCCCTC 
ACCAGAGCCG TCGGTGGCGA TCTCTCCTCC TGCACCTTCC TCTTCGTTGC CGAGGTCTCC 
ATCACCTACA TGGAGACACC GGGAGCCGAC GCCGTCATAC AATGGGCTTC CTCGCTAGGC 
GACTCCGAGT TTGTCCTTCT CGAGCAACTG CTACCTTCGG GGCCGACCCA CCCGTTTGCC 
TCCACAATGC TATCGCATTT TCATAAACTC AACACCCCAA TCAAGAGCGT GGACGTGTAC 
CCTACGGTAG CCTCGCAGGT GGAGCGTTTC CGCTCTCGGG GCTGGGGCAG CGGTGATGTG 
CGCGTCTGGA CGCTGTGGGA GGCGTGGGCA GATGCCGAGG ATACTTTCGT CAATGCGGCG 
GAGAGACGCA GGCTGGACGA GGTGGAGCCG TTCGATGAGT GGGAGGAGTT TGCGCTTTTC 
GCGAGCCATT ATTGTGTGGT GAGGGCCAGG ACAGTGGCTA GGGACGGACA GAATAAGAAG 
AAAGAGAGGG GGATCCCGAA CGGAAGGGAG TTGGGGTTGC CGGTTGAGAA GGTCAAGGTG 
AGGTGGGATG ATGTCCCGGG GCAGAGAGGT CAGAGGAGGT TTGCGGCTGG GGCGGTGTTG 
ACTTCTTCTT CTCCGAGTTC GGAAAAGAAA GAGGAGGTGA AACTGCTGAA CGTTATGGGG 
CTGGGGACGA AGAGCCGGTT GCAGAACTGC GATGTTTACG GACGGAAGAA AGTGGGAGAT 
GCCGACGAGG CTGATGGTAC TGACGAAGAA AAGACGGCGG TGCCGTTCAC TTTCCGTGAG 
GGCGGCCCTT CAACGAGGAT GTGTCACTCG CTGACAGATT TCGGCACTGC CCAACTCCTC 
GTGGGAGGAC GGGCATCACC GTCCACGCCC CTCAAAGACT GCTGGCTGTT GGAGAAGACC 
AACGGCAGCG AGAGTGAATG GGCATGGAAA CGCACCAATG ATCTCCCGAT TCCGCTCTAC 
AGACATTCTG TCACGAGATT GGGGAAGACA GACATGGCGC TGCTTGCAGG TGGGAGGGGA 
GTTGCCGATA TCTTCCCTGA CTGGCTTCTA TATGAGCCCA AACTTGGATG GATCAGATGC 
GAGATTGCCG GTGACGTCAA GCCGACTTCG GTGTATGGCG CTACGCTTGC TTGCCTCCGT 
CAAGAGAGTG ACTCGTTCTC TGGTGTGTTT ACTGGCGGCT TGAGTGATGA TGGCCTCATT 
GCTGATCAGC TTCTCGCATG GAACCTGGAC GTATCCAACA GCAGCAGGCC GGTCGTCACT 
TTTGTTCCGC TACAGGTAAA GTCTGGTGAT GATGGCCGCG AAGAGGCTCT CTCACGTCTA 
CTTCTCACGA GGTTTGGCGC TTCTTGCCTG CCCCAAAGTC GGACCGACTT CTTGGTCTTT 
GGTGGTGTTA TCAAGGACCA CCTTTTGGAT ATGGAAGACG AGATCCTGCT ATGCTCACTC 
AAGGGCGACG GGGAACTCAC AATCACGCGA CGACTCGTTC CCGAGGCCGC TAATGCTAAG 
TCACCATCGC ACCCAGGCCC TTTGTTGTTG GTGGGAACTT CTCCAGTTGT CACGCCGGAC 
GACGGCAGCT TGATGATCGT GGGCGGCGGA GCAACATGCT TCTCCATGGG AACCTTCTGG 
AACAAGGGTA TTTCGACTCT AGAACTGGCA CTGCCAGCTG CGGTGGAGAA TGAAGCTTCA 
CCCATCCCCG CATATGGATG GGTGCACGAG AAAACAGTCG ACATTATCCC TGGTGAACCA 
AGGAGTCTTC CTCTCCGTAA TCAGGCACCC AACGGCGCTG AGGGGAACGC CAATGGCAGC 
GATCGTACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCU05392 ( XM_011395268.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393570.1 Neurospora crassa OR74A leucine carboxyl methyltransferase 2 (NCU05392), mRNA.

Price & Availability↑

CloneID	ONf12466
Clone ID Related Accession (Same CDS sequence)	XM_011395268.1
Accession Version	XM_011395268.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	leucine carboxyl methyltransferase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026502).

ATGGAGACCA CAGAGGAGGT AGTAGCTCCA GCTACGACTC AGCAACCGGC TACCGAAGTC 
GGCTCAGCCA AGCCTACTAC TTCAACATAC AGCAAAAAGC AAAAGAAGCC AAAGGCGACG 
ACGACGACGA CGACGACAGA CGGGACCACG CCAACTCACA ATGAACACGC CAGCGCCAAA 
GACCCGAGAA AAGCTCAGGA TGATCAGGTT ATGGGCACAA ACAACTCGTC CATAGTCTCC 
AAACGCTCCG TCGAAAAACT CTACTACCCC AACGAGCCTC ATTTCTTCCG CTTCTTCGTC 
AAGAAATTCC AGCGACGCGC GCCGCTGATT AATCGCGGTT ATCACTTTCG TTTGCACGTC 
ATCGATGTGC TTGTTCGCAA CTTCTTGCAA GAGCAAAGAA CTGGAGACGC AAAGGGAAAA 
AGAAAAGTGG TGGTTAATTT GGGTTGTGGA AGCGATGTGC TGCCTTGGCA GTGTTTGGCT 
AGGTATCCGG ATGCTTGTCG TTCTGGTGAG AAAGACGGGG CGAAGTTTGT GGATGTCGAT 
TTCCCGGATC TGATTGAGCG GAAGAAGCGG ACGGTTTTGG AGACGCCGGA ACTTTTGGGG 
CCGTTGACGA ACGTGGTGGT ACCAGAGTTT GCTCCTGTGC CTAGTACTCC TGCGGCCACT 
ACTACGGCTG CGGCTACAAC TACCACAACG ACGGAACTCA AAACCACGGC AGCAACCGCT 
TCAAGCACCT CGACCGAAGC CCCTCAAAAG CCCAAGAAAT CCCCCAAACC AAAGGACAAA 
TCCAAAGCCG CCCGCGCCCC GGCCCCCACC ACCGCCCCTA CGGGTATCGT TTTCACCAGC 
GACCAATACG TTCAAATAGG CTGCGACCTG CGTGACCTAG CCACCCTTCA AGACTCCCTC 
ACCAGAGCCG TCGGTGGCGA TCTCTCCTCC TGCACCTTCC TCTTCGTTGC CGAGGTCTCC 
ATCACCTACA TGGAGACACC GGGAGCCGAC GCCGTCATAC AATGGGCTTC CTCGCTAGGC 
GACTCCGAGT TTGTCCTTCT CGAGCAACTG CTACCTTCGG GGCCGACCCA CCCGTTTGCC 
TCCACAATGC TATCGCATTT TCATAAACTC AACACCCCAA TCAAGAGCGT GGACGTGTAC 
CCTACGGTAG CCTCGCAGGT GGAGCGTTTC CGCTCTCGGG GCTGGGGCAG CGGTGATGTG 
CGCGTCTGGA CGCTGTGGGA GGCGTGGGCA GATGCCGAGG ATACTTTCGT CAATGCGGCG 
GAGAGACGCA GGCTGGACGA GGTGGAGCCG TTCGATGAGT GGGAGGAGTT TGCGCTTTTC 
GCGAGCCATT ATTGTGTGGT GAGGGCCAGG ACAGTGGCTA GGGACGGACA GAATAAGAAG 
AAAGAGAGGG GGATCCCGAA CGGAAGGGAG TTGGGGTTGC CGGTTGAGAA GGTCAAGGTG 
AGGTGGGATG ATGTCCCGGG GCAGAGAGGT CAGAGGAGGT TTGCGGCTGG GGCGGTGTTG 
ACTTCTTCTT CTCCGAGTTC GGAAAAGAAA GAGGAGGTGA AACTGCTGAA CGTTATGGGG 
CTGGGGACGA AGAGCCGGTT GCAGAACTGC GATGTTTACG GACGGAAGAA AGTGGGAGAT 
GCCGACGAGG CTGATGGTAC TGACGAAGAA AAGACGGCGG TGCCGTTCAC TTTCCGTGAG 
GGCGGCCCTT CAACGAGGAT GTGTCACTCG CTGACAGATT TCGGCACTGC CCAACTCCTC 
GTGGGAGGAC GGGCATCACC GTCCACGCCC CTCAAAGACT GCTGGCTGTT GGAGAAGACC 
AACGGCAGCG AGAGTGAATG GGCATGGAAA CGCACCAATG ATCTCCCGAT TCCGCTCTAC 
AGACATTCTG TCACGAGATT GGGGAAGACA GACATGGCGC TGCTTGCAGG TGGGAGGGGA 
GTTGCCGATA TCTTCCCTGA CTGGCTTCTA TATGAGCCCA AACTTGGATG GATCAGATGC 
GAGATTGCCG GTGACGTCAA GCCGACTTCG GTGTATGGCG CTACGCTTGC TTGCCTCCGT 
CAAGAGAGTG ACTCGTTCTC TGGTGTGTTT ACTGGCGGCT TGAGTGATGA TGGCCTCATT 
GCTGATCAGC TTCTCGCATG GAACCTGGAC GTATCCAACA GCAGCAGGCC GGTCGTCACT 
TTTGTTCCGC TACAGGTAAA GTCTGGTGAT GATGGCCGCG AAGAGGCTCT CTCACGTCTA 
CTTCTCACGA GGTTTGGCGC TTCTTGCCTG CCCCAAAGTC GGACCGACTT CTTGGTCTTT 
GGTGGTGTTA TCAAGGACCA CCTTTTGGAT ATGGAAGACG AGATCCTGCT ATGCTCACTC 
AAGGGCGACG GGGAACTCAC AATCACGCGA CGACTCGTTC CCGAGGCCGC TAATGCTAAG 
TCACCATCGC ACCCAGGCCC TTTGTTGTTG GTGGGAACTT CTCCAGTTGT CACGCCGGAC 
GACGGCAGCT TGATGATCGT GGGCGGCGGA GCAACATGCT TCTCCATGGG AACCTTCTGG 
AACAAGGGTA TTTCGACTCT AGAACTGGCA CTGCCAGCTG CGGTGGAGAA TGAAGCTTCA 
CCCATCCCCG CATATGGATG GGTGCACGAG AAAACAGTCG ACATTATCCC TGGTGAACCA 
AGGAGTCTTC CTCTCCGTAA TCAGGCACCC AACGGCGCTG AGGGGAACGC CAATGGCAGC 
GTAAGCGTCC GCATTCAACC CATACCCCGG GTCAGGTTGG AAAATGCTGA AGACTTCGCC 
AGGATCGTAC GTGAGGGTCG TCCGGTGGTT CTGGAGGGCC TAAATCTGGG TGATTGTGTG 
TCACAATGGG GAAACGGTGA CTACGTTGCG AAGAAGGTTG GGACAGACCG CAAGGTCGTG 
ATCCACGAGT CCACGACACC TGCAATGGAT TTCACCACCA AGAACTTCCG CTACGTGACG 
ACCGAGTTTG GCGACTTCAT GAGGCGCGTT GAGAAAGGTG ACCGACTGTA CTTGAGGGCG 
CTATCGACCG ACAAACCGAC AGAAAAGCCA GCCGTTCTAA GTGACGACTT TCCCTCGTTG 
GCTACTGATT TTGTGTTGCC GCCTGAGCTG GCCTTGGTAG GCGACAGACT GTTCAGCTCG 
GTCCTACGAG TATCTGGGCC GGTCAACATG TGGTTGCATT ACGATGTCAT GGCCAATGTG 
TACTGCCAAA TCGGCGGCTC GAAGCGCATG ATTCTTTTCC CGCCGTCCGA TGTTGAGCAT 
CTCGGCTTTG CTCCCGGGGC ATCGAGTTCC AGTATCGATG TCTTTTCTAC TCTTTTCGGG 
GAGTCGAGTG ACTCGCGATA CTTGGCTCAA GTGACGCATC CTCACGAGGC TGTCATGACT 
CCAGGAGACG TCCTCTTCTT GCCCCCACTC TGGCTGCACA CGGCGACTCC TACGTCGGAT 
AGCAGCATTG CGGTGAACGT CTTCTTCCGC GATCTCGAAG GCGGTTGCTA TGCAGCAGGG 
AAAGATGTGT ATGGGAACAG AGATCTGGCG GCCTATGAAA AGGGCCGAAC GGACTTGACT 
CGTATTGCAA ACAGCTTTCA GAAGCTGCCG GCTGAGGCGA GGGAGTTTTA TCTGTTGAGA 
CTTGCAGACG AGCTTCGGAG GAAAGCCAAG GGAGCCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393570.1
CDS	63..3704
Translation	METTEEVVAPATTQQPATEVGSAKPTTSTYSKKQKKPKATTTTTTTDGTTPTHNEHASAKDPRKAQDDQVMGTNNSSIVSKRSVEKLYYPNEPHFFRFFVKKFQRRAPLINRGYHFRLHVIDVLVRNFLQEQRTGDAKGKRKVVVNLGCGSDVLPWQCLARYPDACRSGEKDGAKFVDVDFPDLIERKKRTVLETPELLGPLTNVVVPEFAPVPSTPAATTTAAATTTTTTELKTTAATASSTSTEAPQKPKKSPKPKDKSKAARAPAPTTAPTGIVFTSDQYVQIGCDLRDLATLQDSLTRAVGGDLSSCTFLFVAEVSITYMETPGADAVIQWASSLGDSEFVLLEQLLPSGPTHPFASTMLSHFHKLNTPIKSVDVYPTVASQVERFRSRGWGSGDVRVWTLWEAWADAEDTFVNAAERRRLDEVEPFDEWEEFALFASHYCVVRARTVARDGQNKKKERGIPNGRELGLPVEKVKVRWDDVPGQRGQRRFAAGAVLTSSSPSSEKKEEVKLLNVMGLGTKSRLQNCDVYGRKKVGDADEADGTDEEKTAVPFTFREGGPSTRMCHSLTDFGTAQLLVGGRASPSTPLKDCWLLEKTNGSESEWAWKRTNDLPIPLYRHSVTRLGKTDMALLAGGRGVADIFPDWLLYEPKLGWIRCEIAGDVKPTSVYGATLACLRQESDSFSGVFTGGLSDDGLIADQLLAWNLDVSNSSRPVVTFVPLQVKSGDDGREEALSRLLLTRFGASCLPQSRTDFLVFGGVIKDHLLDMEDEILLCSLKGDGELTITRRLVPEAANAKSPSHPGPLLLVGTSPVVTPDDGSLMIVGGGATCFSMGTFWNKGISTLELALPAAVENEASPIPAYGWVHEKTVDIIPGEPRSLPLRNQAPNGAEGNANGSVSVRIQPIPRVRLENAEDFARIVREGRPVVLEGLNLGDCVSQWGNGDYVAKKVGTDRKVVIHESTTPAMDFTTKNFRYVTTEFGDFMRRVEKGDRLYLRALSTDKPTEKPAVLSDDFPSLATDFVLPPELALVGDRLFSSVLRVSGPVNMWLHYDVMANVYCQIGGSKRMILFPPSDVEHLGFAPGASSSSIDVFSTLFGESSDSRYLAQVTHPHEAVMTPGDVLFLPPLWLHTATPTSDSSIAVNVFFRDLEGGCYAAGKDVYGNRDLAAYEKGRTDLTRIANSFQKLPAEAREFYLLRLADELRRKAKGAQ

Target ORF information:

RefSeq Version	XM_011395268.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A leucine carboxyl methyltransferase 2 (NCU05392), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395268.1

ORF Insert Sequence:

ATGGAGACCA CAGAGGAGGT AGTAGCTCCA GCTACGACTC AGCAACCGGC TACCGAAGTC 
GGCTCAGCCA AGCCTACTAC TTCAACATAC AGCAAAAAGC AAAAGAAGCC AAAGGCGACG 
ACGACGACGA CGACGACAGA CGGGACCACG CCAACTCACA ATGAACACGC CAGCGCCAAA 
GACCCGAGAA AAGCTCAGGA TGATCAGGTT ATGGGCACAA ACAACTCGTC CATAGTCTCC 
AAACGCTCCG TCGAAAAACT CTACTACCCC AACGAGCCTC ATTTCTTCCG CTTCTTCGTC 
AAGAAATTCC AGCGACGCGC GCCGCTGATT AATCGCGGTT ATCACTTTCG TTTGCACGTC 
ATCGATGTGC TTGTTCGCAA CTTCTTGCAA GAGCAAAGAA CTGGAGACGC AAAGGGAAAA 
AGAAAAGTGG TGGTTAATTT GGGTTGTGGA AGCGATGTGC TGCCTTGGCA GTGTTTGGCT 
AGGTATCCGG ATGCTTGTCG TTCTGGTGAG AAAGACGGGG CGAAGTTTGT GGATGTCGAT 
TTCCCGGATC TGATTGAGCG GAAGAAGCGG ACGGTTTTGG AGACGCCGGA ACTTTTGGGG 
CCGTTGACGA ACGTGGTGGT ACCAGAGTTT GCTCCTGTGC CTAGTACTCC TGCGGCCACT 
ACTACGGCTG CGGCTACAAC TACCACAACG ACGGAACTCA AAACCACGGC AGCAACCGCT 
TCAAGCACCT CGACCGAAGC CCCTCAAAAG CCCAAGAAAT CCCCCAAACC AAAGGACAAA 
TCCAAAGCCG CCCGCGCCCC GGCCCCCACC ACCGCCCCTA CGGGTATCGT TTTCACCAGC 
GACCAATACG TTCAAATAGG CTGCGACCTG CGTGACCTAG CCACCCTTCA AGACTCCCTC 
ACCAGAGCCG TCGGTGGCGA TCTCTCCTCC TGCACCTTCC TCTTCGTTGC CGAGGTCTCC 
ATCACCTACA TGGAGACACC GGGAGCCGAC GCCGTCATAC AATGGGCTTC CTCGCTAGGC 
GACTCCGAGT TTGTCCTTCT CGAGCAACTG CTACCTTCGG GGCCGACCCA CCCGTTTGCC 
TCCACAATGC TATCGCATTT TCATAAACTC AACACCCCAA TCAAGAGCGT GGACGTGTAC 
CCTACGGTAG CCTCGCAGGT GGAGCGTTTC CGCTCTCGGG GCTGGGGCAG CGGTGATGTG 
CGCGTCTGGA CGCTGTGGGA GGCGTGGGCA GATGCCGAGG ATACTTTCGT CAATGCGGCG 
GAGAGACGCA GGCTGGACGA GGTGGAGCCG TTCGATGAGT GGGAGGAGTT TGCGCTTTTC 
GCGAGCCATT ATTGTGTGGT GAGGGCCAGG ACAGTGGCTA GGGACGGACA GAATAAGAAG 
AAAGAGAGGG GGATCCCGAA CGGAAGGGAG TTGGGGTTGC CGGTTGAGAA GGTCAAGGTG 
AGGTGGGATG ATGTCCCGGG GCAGAGAGGT CAGAGGAGGT TTGCGGCTGG GGCGGTGTTG 
ACTTCTTCTT CTCCGAGTTC GGAAAAGAAA GAGGAGGTGA AACTGCTGAA CGTTATGGGG 
CTGGGGACGA AGAGCCGGTT GCAGAACTGC GATGTTTACG GACGGAAGAA AGTGGGAGAT 
GCCGACGAGG CTGATGGTAC TGACGAAGAA AAGACGGCGG TGCCGTTCAC TTTCCGTGAG 
GGCGGCCCTT CAACGAGGAT GTGTCACTCG CTGACAGATT TCGGCACTGC CCAACTCCTC 
GTGGGAGGAC GGGCATCACC GTCCACGCCC CTCAAAGACT GCTGGCTGTT GGAGAAGACC 
AACGGCAGCG AGAGTGAATG GGCATGGAAA CGCACCAATG ATCTCCCGAT TCCGCTCTAC 
AGACATTCTG TCACGAGATT GGGGAAGACA GACATGGCGC TGCTTGCAGG TGGGAGGGGA 
GTTGCCGATA TCTTCCCTGA CTGGCTTCTA TATGAGCCCA AACTTGGATG GATCAGATGC 
GAGATTGCCG GTGACGTCAA GCCGACTTCG GTGTATGGCG CTACGCTTGC TTGCCTCCGT 
CAAGAGAGTG ACTCGTTCTC TGGTGTGTTT ACTGGCGGCT TGAGTGATGA TGGCCTCATT 
GCTGATCAGC TTCTCGCATG GAACCTGGAC GTATCCAACA GCAGCAGGCC GGTCGTCACT 
TTTGTTCCGC TACAGGTAAA GTCTGGTGAT GATGGCCGCG AAGAGGCTCT CTCACGTCTA 
CTTCTCACGA GGTTTGGCGC TTCTTGCCTG CCCCAAAGTC GGACCGACTT CTTGGTCTTT 
GGTGGTGTTA TCAAGGACCA CCTTTTGGAT ATGGAAGACG AGATCCTGCT ATGCTCACTC 
AAGGGCGACG GGGAACTCAC AATCACGCGA CGACTCGTTC CCGAGGCCGC TAATGCTAAG 
TCACCATCGC ACCCAGGCCC TTTGTTGTTG GTGGGAACTT CTCCAGTTGT CACGCCGGAC 
GACGGCAGCT TGATGATCGT GGGCGGCGGA GCAACATGCT TCTCCATGGG AACCTTCTGG 
AACAAGGGTA TTTCGACTCT AGAACTGGCA CTGCCAGCTG CGGTGGAGAA TGAAGCTTCA 
CCCATCCCCG CATATGGATG GGTGCACGAG AAAACAGTCG ACATTATCCC TGGTGAACCA 
AGGAGTCTTC CTCTCCGTAA TCAGGCACCC AACGGCGCTG AGGGGAACGC CAATGGCAGC 
GTAAGCGTCC GCATTCAACC CATACCCCGG GTCAGGTTGG AAAATGCTGA AGACTTCGCC 
AGGATCGTAC GTGAGGGTCG TCCGGTGGTT CTGGAGGGCC TAAATCTGGG TGATTGTGTG 
TCACAATGGG GAAACGGTGA CTACGTTGCG AAGAAGGTTG GGACAGACCG CAAGGTCGTG 
ATCCACGAGT CCACGACACC TGCAATGGAT TTCACCACCA AGAACTTCCG CTACGTGACG 
ACCGAGTTTG GCGACTTCAT GAGGCGCGTT GAGAAAGGTG ACCGACTGTA CTTGAGGGCG 
CTATCGACCG ACAAACCGAC AGAAAAGCCA GCCGTTCTAA GTGACGACTT TCCCTCGTTG 
GCTACTGATT TTGTGTTGCC GCCTGAGCTG GCCTTGGTAG GCGACAGACT GTTCAGCTCG 
GTCCTACGAG TATCTGGGCC GGTCAACATG TGGTTGCATT ACGATGTCAT GGCCAATGTG 
TACTGCCAAA TCGGCGGCTC GAAGCGCATG ATTCTTTTCC CGCCGTCCGA TGTTGAGCAT 
CTCGGCTTTG CTCCCGGGGC ATCGAGTTCC AGTATCGATG TCTTTTCTAC TCTTTTCGGG 
GAGTCGAGTG ACTCGCGATA CTTGGCTCAA GTGACGCATC CTCACGAGGC TGTCATGACT 
CCAGGAGACG TCCTCTTCTT GCCCCCACTC TGGCTGCACA CGGCGACTCC TACGTCGGAT 
AGCAGCATTG CGGTGAACGT CTTCTTCCGC GATCTCGAAG GCGGTTGCTA TGCAGCAGGG 
AAAGATGTGT ATGGGAACAG AGATCTGGCG GCCTATGAAA AGGGCCGAAC GGACTTGACT 
CGTATTGCAA ACAGCTTTCA GAAGCTGCCG GCTGAGGCGA GGGAGTTTTA TCTGTTGAGA 
CTTGCAGACG AGCTTCGGAG GAAAGCCAAG GGAGCCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU05392 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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