Lcmt2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lcmt2
Entrez Gene ID	329504
Full Name	leucine carboxyl methyltransferase 2
Synonyms	D330024M17,Tyw4
General protein information	Preferred Names leucine carboxyl methyltransferase 2 Names tRNA wybutosine-synthesizing protein 4 p21WAF1/CIP1 promoter-interacting protein tRNA wybutosine-synthesizing protein 4 homolog tRNA yW-synthesizing protein 4 homolog tRNA(Phe) (7-(3-amino-3-(methoxycarbonyl)propyl)wyosine(37)-N)-methoxycarbonyltransferase tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 E5

mRNA and Protein(s)

mRNA	Protein	Name
NM_177846.3	NP_808514.2	tRNA wybutosine-synthesizing protein 4

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LCMT2	XP_417263.3
Saccharomyces cerevisiae (baker's yeast)	PPM2	NP_014500.2
Magnaporthe oryzae (rice blast fungus)	MGG_03140	XP_003716857.1
Rattus norvegicus (Norway rat)	Lcmt2	NP_001011956.1
Homo sapiens (human)	LCMT2	NP_055608.2
Canis lupus familiaris (dog)	LCMT2	XP_003640071.1
Mus musculus (house mouse)	Lcmt2	NP_808514.2
Schizosaccharomyces pombe (fission yeast)	SPBC19C7.08c	NP_596164.1
Xenopus tropicalis (tropical clawed frog)	lcmt2	NP_001119971.1
	LCMT2	XP_001106399.1
Bos taurus (cattle)	LCMT2	XP_002696850.1
Neurospora crassa	NCU05392	XP_963480.2
Danio rerio (zebrafish)	lcmt2	NP_001122029.1
Eremothecium gossypii	AGOS_ADL194W	NP_983902.1

Gene Ontology
Bibliography

Related articles in PubMed

Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, F-Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Seino S, Nishimura M, Kaisho T, Hoshino K, Kitamura H, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes11(3)205-18(2004 Jun)

Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
Piao Y, Ko NT, Lim MK, Ko MS
Genome research11(9)1553-8(2001 Sep)

Purification of porcine brain protein phosphatase 2A leucine carboxyl methyltransferase and cloning of the human homologue.
De Baere I, Derua R, Janssens V, Van Hoof C, Waelkens E, Merlevede W, Goris J
Biochemistry38(50)16539-47(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Lcmt2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lcmt2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu21583	NM_177846.3 Latest version!	Mus musculus leucine carboxyl methyltransferase 2 (Lcmt2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lcmt2 ( NM_177846.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_808514.2 Mus musculus leucine carboxyl methyltransferase 2 (Lcmt2), mRNA.

Price & Availability↑

CloneID	OMu21583
Clone ID Related Accession (Same CDS sequence)	NM_177846.3
Accession Version	NM_177846.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Mus musculus(house mouse)
Product	tRNA wybutosine-synthesizing protein 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL845479.8. On or before Sep 7, 2008 this sequence version replaced XM_001003239.1, XM_141327.6, NM_177846.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

ATGGGCCCGC GAGGCCGCCA GCGCCGGGCG GGAACGGTCC AGAGCACCAA CGACAGCAGC 
TCCCTCAGCA AACGATCGCT TGCCGCACAC GGATACGTGC GCGACCCCTT TGCGGCGCTG 
CTGGTCCCGG GACCCGTGCG GCGCACGCCG CTTATCCACC GCGGCTATTA CGTGCGCGCG 
CGCGCCGTGC GTCACTGCGT GCGCGCCTTC CTCGAACTCA CTAGCGCGCT CCCGTCCCGG 
ACCAGAGCCC AGATCCTGTC CTTGGGCTCA GGCTCCGACT CGCTTTATTT TCGTCTGAAA 
GCGGCGGGCC TCCTGGCCCG AGCTGCCGTC TGGGAGGTGG ACTTCCCGGA CGTGTCTCGG 
CTCAAGGCGG AGAGGATCGA GGAGACCCCG GAGCTGCGTG CGCAGACCGG TCCTTTCAAG 
ATCGGGGACT CGGCGTCATC TCTGTGCTTC GAGAGCGCGG ACTACCGCAT CCTGGGCGCG 
GACCTGCGGG AGCTCCAGCG ATTAGGAGAG GCCCTGGATG GCGCCGGCCT GGACGCCACC 
TCTCCCACGC TGCTCCTGGC CGAGGCGGTG CTGACCTACT TGGAGCCCTC CAGTGCCACA 
GCCCTCATTG CCTGGGCCGC CCAGCGTTTC CCCGACGCCC TTTTCGTGAT CTATGAGCAG 
ATGCAGCCGG GGGATGCCTT TGGGCAGATC ATGCTGCAGC ACTTTCAGCG GTTGCATTCA 
CCCTTGCATG GCCTGGAGCT GTTTCCCGTC GTGAAGGCCC AGCGCCAGCG CTTCCTTCAA 
GCTGGCTGGA CTGCCTGCAG CGCCCTGGAC CTGAACGAGT TCTATCGCCG CCTTCTCTCG 
GCAGAAGAGC GCCAGCGGGT TGAGACGCTT GAACCCTTTG ATGAATATGA GGAGTGGCAT 
CTGAAGTGTT CACATTATTT CATCCTGGCA GCATCTAGGG GAGACATTCT GTCAGAAACT 
CCAGTGTTTG AGCCCTCAGA GGCTTCTTTT CAGATAGATC CTGCCTCGCC TTCAGGGTTT 
CTTTCTGCCA GAGTAGTCAC TAGTGACCAC CAGCACTCAA GCCTGAAGCG ATATGGCCAC 
GCCTCTGCCC TCTTGAGCCC TGGCGTTATT TTCAGTGCAG GAGGCTTTGG AGAACAAGAG 
GGACGACACT GCCGAGTGAG CAGGTTTCAC GTGCTCTCCA GATCCTGTGA CTCGGAATGG 
GAAGGCTGCC AAATAAGTAC CTTGGGGACT GAAGGCCAGT GGGATGGACG CCTTTATCAC 
ACCATGACAA GGCTTTCTGA TACTCGGGTT CTGGTTCTTG GAGGAAGACT CTCCCCAGTA 
AGTCCAGCCT CTGGGGCTCT CCAACTTGAT TTATACAAAA GTAAGGATAA TTGCTCTGAG 
GGCCAAAATG TAACAGTAAC GAAGGCTGCC TTGGAAGAAG GTTCAGTGTT GTCATGTTGG 
CGGCATTCAA CAACGGAAGT ATACTATCAG AATCAAAGAT ACTTATTTGT GTATGGTGGC 
CGAAGTGTGG CTGAACCTGT ACTAAGTGAC TGTCGTTTTC TACATGTAGA GACAATGGCT 
TGGGTCAGAA TCCCAGTACA AGGCGCATCA CCCGAAGGTC GGCATTCCCA TAGCGCCTGC 
AGTTGGCAAG GGGGAGCACT TATCGCTGGA GGTCTTGGGG CTTCTGAGGA ACTGTTGAGC 
TCTGTACTCT TTCTCAAACC AGTGTCCTCT GGATTCCTCT GGGAATCAAT AGATATCCAG 
CCCTCCATTA CCCCAAGGTA CTCTCACACG GCTCATGTGT TTAATGGAAA GCTGCTTCTG 
GTTGGAGGGG TCTGGATTCA TTCCTCCTCA GTTCCTGGAG TGACTGTTAT CTGTTTGACT 
ACAGGGTTGA GCTCTGAATA TCAGATTGAC ACAGCGTCTG TGCCATGGCC ATTAATGCTG 
CACAACCACA GCAGCGCCCT CCTTCCTGAA GAGCAGCAGC TCCTGCTTAT TGGAGGTGGA 
GGGAACTGCT TTTCCTTTGG TACTTATTTC AACCCCCACA CAGTGGCATT AGACCTTTCT 
TCCTTACGTT CAGGGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808514.2
CDS	99..2159
Translation	MGPRGRQRRAGTVQSTNDSSSLSKRSLAAHGYVRDPFAALLVPGPVRRTPLIHRGYYVRARAVRHCVRAFLELTSALPSRTRAQILSLGSGSDSLYFRLKAAGLLARAAVWEVDFPDVSRLKAERIEETPELRAQTGPFKIGDSASSLCFESADYRILGADLRELQRLGEALDGAGLDATSPTLLLAEAVLTYLEPSSATALIAWAAQRFPDALFVIYEQMQPGDAFGQIMLQHFQRLHSPLHGLELFPVVKAQRQRFLQAGWTACSALDLNEFYRRLLSAEERQRVETLEPFDEYEEWHLKCSHYFILAASRGDILSETPVFEPSEASFQIDPASPSGFLSARVVTSDHQHSSLKRYGHASALLSPGVIFSAGGFGEQEGRHCRVSRFHVLSRSCDSEWEGCQISTLGTEGQWDGRLYHTMTRLSDTRVLVLGGRLSPVSPASGALQLDLYKSKDNCSEGQNVTVTKAALEEGSVLSCWRHSTTEVYYQNQRYLFVYGGRSVAEPVLSDCRFLHVETMAWVRIPVQGASPEGRHSHSACSWQGGALIAGGLGASEELLSSVLFLKPVSSGFLWESIDIQPSITPRYSHTAHVFNGKLLLVGGVWIHSSSVPGVTVICLTTGLSSEYQIDTASVPWPLMLHNHSSALLPEEQQLLLIGGGGNCFSFGTYFNPHTVALDLSSLRSGQ

Target ORF information:

RefSeq Version	NM_177846.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine carboxyl methyltransferase 2 (Lcmt2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177846.3

ORF Insert Sequence:

ATGGGCCCGC GAGGCCGCCA GCGCCGGGCG GGAACGGTCC AGAGCACCAA CGACAGCAGC 
TCCCTCAGCA AACGATCGCT TGCCGCACAC GGATACGTGC GCGACCCCTT TGCGGCGCTG 
CTGGTCCCGG GACCCGTGCG GCGCACGCCG CTTATCCACC GCGGCTATTA CGTGCGCGCG 
CGCGCCGTGC GTCACTGCGT GCGCGCCTTC CTCGAACTCA CTAGCGCGCT CCCGTCCCGG 
ACCAGAGCCC AGATCCTGTC CTTGGGCTCA GGCTCCGACT CGCTTTATTT TCGTCTGAAA 
GCGGCGGGCC TCCTGGCCCG AGCTGCCGTC TGGGAGGTGG ACTTCCCGGA CGTGTCTCGG 
CTCAAGGCGG AGAGGATCGA GGAGACCCCG GAGCTGCGTG CGCAGACCGG TCCTTTCAAG 
ATCGGGGACT CGGCGTCATC TCTGTGCTTC GAGAGCGCGG ACTACCGCAT CCTGGGCGCG 
GACCTGCGGG AGCTCCAGCG ATTAGGAGAG GCCCTGGATG GCGCCGGCCT GGACGCCACC 
TCTCCCACGC TGCTCCTGGC CGAGGCGGTG CTGACCTACT TGGAGCCCTC CAGTGCCACA 
GCCCTCATTG CCTGGGCCGC CCAGCGTTTC CCCGACGCCC TTTTCGTGAT CTATGAGCAG 
ATGCAGCCGG GGGATGCCTT TGGGCAGATC ATGCTGCAGC ACTTTCAGCG GTTGCATTCA 
CCCTTGCATG GCCTGGAGCT GTTTCCCGTC GTGAAGGCCC AGCGCCAGCG CTTCCTTCAA 
GCTGGCTGGA CTGCCTGCAG CGCCCTGGAC CTGAACGAGT TCTATCGCCG CCTTCTCTCG 
GCAGAAGAGC GCCAGCGGGT TGAGACGCTT GAACCCTTTG ATGAATATGA GGAGTGGCAT 
CTGAAGTGTT CACATTATTT CATCCTGGCA GCATCTAGGG GAGACATTCT GTCAGAAACT 
CCAGTGTTTG AGCCCTCAGA GGCTTCTTTT CAGATAGATC CTGCCTCGCC TTCAGGGTTT 
CTTTCTGCCA GAGTAGTCAC TAGTGACCAC CAGCACTCAA GCCTGAAGCG ATATGGCCAC 
GCCTCTGCCC TCTTGAGCCC TGGCGTTATT TTCAGTGCAG GAGGCTTTGG AGAACAAGAG 
GGACGACACT GCCGAGTGAG CAGGTTTCAC GTGCTCTCCA GATCCTGTGA CTCGGAATGG 
GAAGGCTGCC AAATAAGTAC CTTGGGGACT GAAGGCCAGT GGGATGGACG CCTTTATCAC 
ACCATGACAA GGCTTTCTGA TACTCGGGTT CTGGTTCTTG GAGGAAGACT CTCCCCAGTA 
AGTCCAGCCT CTGGGGCTCT CCAACTTGAT TTATACAAAA GTAAGGATAA TTGCTCTGAG 
GGCCAAAATG TAACAGTAAC GAAGGCTGCC TTGGAAGAAG GTTCAGTGTT GTCATGTTGG 
CGGCATTCAA CAACGGAAGT ATACTATCAG AATCAAAGAT ACTTATTTGT GTATGGTGGC 
CGAAGTGTGG CTGAACCTGT ACTAAGTGAC TGTCGTTTTC TACATGTAGA GACAATGGCT 
TGGGTCAGAA TCCCAGTACA AGGCGCATCA CCCGAAGGTC GGCATTCCCA TAGCGCCTGC 
AGTTGGCAAG GGGGAGCACT TATCGCTGGA GGTCTTGGGG CTTCTGAGGA ACTGTTGAGC 
TCTGTACTCT TTCTCAAACC AGTGTCCTCT GGATTCCTCT GGGAATCAAT AGATATCCAG 
CCCTCCATTA CCCCAAGGTA CTCTCACACG GCTCATGTGT TTAATGGAAA GCTGCTTCTG 
GTTGGAGGGG TCTGGATTCA TTCCTCCTCA GTTCCTGGAG TGACTGTTAT CTGTTTGACT 
ACAGGGTTGA GCTCTGAATA TCAGATTGAC ACAGCGTCTG TGCCATGGCC ATTAATGCTG 
CACAACCACA GCAGCGCCCT CCTTCCTGAA GAGCAGCAGC TCCTGCTTAT TGGAGGTGGA 
GGGAACTGCT TTTCCTTTGG TACTTATTTC AACCCCCACA CAGTGGCATT AGACCTTTCT 
TCCTTACGTT CAGGGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes11(3)205-18(2004 Jun)
Okazaki N,F-Kikuno R,Ohara R,Inamoto S,Koseki H,Hiraoka S,Saga Y,Seino S,Nishimura M,Kaisho T,Hoshino K,Kitamura H,Nagase T,Ohara O,Koga H

Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
Genome research11(9)1553-8(2001 Sep)
Piao Y,Ko NT,Lim MK,Ko MS

Purification of porcine brain protein phosphatase 2A leucine carboxyl methyltransferase and cloning of the human homologue.
Biochemistry38(50)16539-47(1999 Dec)
De Baere I,Derua R,Janssens V,Van Hoof C,Waelkens E,Merlevede W,Goris J

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Lcmt2 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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