GIT2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GIT2
Entrez Gene ID	9815
Full Name	GIT ArfGAP 2
Synonyms	CAT-2,CAT2,PKL
General protein information	Preferred Names GIT ArfGAP 2 Names ARF GTPase-activating protein GIT2 ARF GAP GIT2 G protein-coupled receptor kinase interacting ArfGAP 2 G protein-coupled receptor kinase-interactor 2 GRK-interacting protein 2 Paxillin kinase linker cool-associated, tyrosine phosphorylated protein 2 cool-interacting tyrosine-phosphorylated protein 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q24.11
Summary	This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. [provided by RefSeq, Sep 2008].
Disorder MIM:	608564

mRNA and Protein(s)

mRNA	Protein	Name
NM_001330153.1	NP_001317082.1	ARF GTPase-activating protein GIT2 isoform 7
XM_006719707.4	XP_006719770.1	ARF GTPase-activating protein GIT2 isoform X2
XM_006719709.4	XP_006719772.1	ARF GTPase-activating protein GIT2 isoform X5
XM_006719712.4	XP_006719775.1	ARF GTPase-activating protein GIT2 isoform X8
NM_001330154.1	NP_001317083.1	ARF GTPase-activating protein GIT2 isoform 8
XM_006719708.3	XP_006719771.1	ARF GTPase-activating protein GIT2 isoform X3
XM_017020258.1	XP_016875747.1	ARF GTPase-activating protein GIT2 isoform X4
NM_057169.4	NP_476510.1	ARF GTPase-activating protein GIT2 isoform 1
NM_057169.3	NP_476510.1	ARF GTPase-activating protein GIT2 isoform 1
NM_001135214.3	NP_001128686.1	ARF GTPase-activating protein GIT2 isoform 5
NM_001135213.3	NP_001128685.1	ARF GTPase-activating protein GIT2 isoform 6
XM_017020258.2	XP_016875747.1	ARF GTPase-activating protein GIT2 isoform X4
XM_017020259.2	XP_016875748.1	ARF GTPase-activating protein GIT2 isoform X6
XM_017020261.2	XP_016875750.1	ARF GTPase-activating protein GIT2 isoform X9
XM_006719709.3	XP_006719772.1	ARF GTPase-activating protein GIT2 isoform X5
XM_017020260.1	XP_016875749.1	ARF GTPase-activating protein GIT2 isoform X8
XM_006719712.3	XP_006719775.1	ARF GTPase-activating protein GIT2 isoform X9
NM_057170.3	NP_476511.1	ARF GTPase-activating protein GIT2 isoform 2
NM_001135214.2	NP_001128686.1	ARF GTPase-activating protein GIT2 isoform 5
NM_001330154.2	NP_001317083.1	ARF GTPase-activating protein GIT2 isoform 8
NM_139201.3	NP_631940.2	ARF GTPase-activating protein GIT2 isoform 4
NM_057169.5	NP_476510.1	ARF GTPase-activating protein GIT2 isoform 1
XM_017020260.2	XP_016875749.1	ARF GTPase-activating protein GIT2 isoform X7
XM_017020261.1	XP_016875750.1	ARF GTPase-activating protein GIT2 isoform X10
XM_017020259.1	XP_016875748.1	ARF GTPase-activating protein GIT2 isoform X7
NM_014776.4	NP_055591.2	ARF GTPase-activating protein GIT2 isoform 3
NM_001135213.2	NP_001128685.1	ARF GTPase-activating protein GIT2 isoform 6
NM_057170.5	NP_476511.1	ARF GTPase-activating protein GIT2 isoform 2
XM_005253997.4	XP_005254054.1	ARF GTPase-activating protein GIT2 isoform X1
XM_006719713.4	XP_006719776.1	ARF GTPase-activating protein GIT2 isoform X10
NM_139201.2	NP_631940.2	ARF GTPase-activating protein GIT2 isoform 4
XM_006719713.3	XP_006719776.1	ARF GTPase-activating protein GIT2 isoform X11
XM_006719707.3	XP_006719770.1	ARF GTPase-activating protein GIT2 isoform X2
XM_005253997.3	XP_005254054.1	ARF GTPase-activating protein GIT2 isoform X1
NM_057170.4	NP_476511.1	ARF GTPase-activating protein GIT2 isoform 2
NM_014776.5	NP_055591.2	ARF GTPase-activating protein GIT2 isoform 3
NM_001330153.2	NP_001317082.1	ARF GTPase-activating protein GIT2 isoform 7
XM_006719708.4	XP_006719771.1	ARF GTPase-activating protein GIT2 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	GIT2	NP_001128686.1
Canis lupus familiaris (dog)	GIT2	XP_005636373.1
Rattus norvegicus (Norway rat)	Git2	NP_001005553.1
Danio rerio (zebrafish)	git2a	NP_001119925.1
Bos taurus (cattle)	GIT2	NP_001180030.1
Drosophila melanogaster (fruit fly)	Git	NP_610599.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006888	XP_308871.4
	GIT2	XP_001106038.1
Gallus gallus (chicken)	GIT2	NP_989537.1
Pan troglodytes (chimpanzee)	GIT2	XP_001139823.2
Mus musculus (house mouse)	Git2	NP_001070827.1
Xenopus tropicalis (tropical clawed frog)	git2	NP_001016917.1

Gene Ontology
Bibliography

Related articles in PubMed

The focal adhesion-associated proteins DOCK5 and GIT2 comprise a rheostat in control of epithelial invasion.
Frank SR, K?llmann CP, van Lidth de Jeude JF, Thiagarajah JR, Engelholm LH, Fr?din M, Hansen SH
Oncogene36(13)1816-1828(2017 Mar)

EGF-stimulated activation of Rab35 regulates RUSC2-GIT2 complex formation to stabilize GIT2 during directional lung cancer cell migration.
Duan B, Cui J, Sun S, Zheng J, Zhang Y, Ye B, Chen Y, Deng W, Du J, Zhu Y, Chen Y, Gu L
Cancer letters379(1)70-83(2016 08)

Expanding functions of GIT Arf GTPase-activating proteins, PIX Rho guanine nucleotide exchange factors and GIT-PIX complexes.
Zhou W, Li X, Premont RT
Journal of cell science129(10)1963-74(2016 05)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

Nuclear GIT2 is an ATM substrate and promotes DNA repair.
Lu D, Cai H, Park SS, Siddiqui S, Premont RT, Schmalzigaug R, Paramasivam M, Seidman M, Bodogai I, Biragyn A, Daimon CM, Martin B, Maudsley S
Molecular and cellular biology35(7)1081-96(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our work identifies DOCK5 as a key regulator of epithelial invasion and metastasis, and demonstrates that suppression of DOCK5 by GIT2 represents a previously unappreciated mechanism for coordination of Rho and Rac GTPases.
Title: The focal adhesion-associated proteins DOCK5 and GIT2 comprise a rheostat in control of epithelial invasion.

Short-term EGF stimulation if of lung tumor cells can increase the interaction between RUSC2 and GIT2, prolonged stimulation leads to a decrease of their interaction through activating Rab35.
Title: EGF-stimulated activation of Rab35 regulates RUSC2-GIT2 complex formation to stabilize GIT2 during directional lung cancer cell migration.

we discuss recent findings in key physiological systems that exemplify current understanding of the function of this important regulatory complex. Further, we draw attention to gaps in crucial information that remain to be filled to allow a better understanding of the many roles of the GIT-PIX complex in health and disease
Title: Expanding functions of GIT Arf GTPase-activating proteins, PIX Rho guanine nucleotide exchange factors and GIT-PIX complexes.

GIT2 plays an important role in MRE11/ATM/H2AX-mediated DNA damage responses.
Title: Nuclear GIT2 is an ATM substrate and promotes DNA repair.

ADMA and L-arginine are substrates of human CAT2A, CAT2B, OCT2 and MATE1. Transport kinetics of CAT2A, CAT2B, and OCT2 indicate a low affinity, high capacity transport, which may be relevant for renal and hepatic elimination of ADMA or L-arginine
Title: Transport of asymmetric dimethylarginine (ADMA) by cationic amino acid transporter 2 (CAT2), organic cation transporter 2 (OCT2) and multidrug and toxin extrusion protein 1 (MATE1).

The following GIT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GIT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19439	NM_057169.5 Latest version!	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$384.50-$538.30 $769.00
OHu13926	NM_057170.5 Latest version!	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu13992	NM_139201.3 Latest version!	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu47003	NM_001330154.2 Latest version!	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu47001	XM_006719712.4 Latest version!	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu47002	XM_006719713.4 Latest version!	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu84347	XM_017020261.2 Latest version!	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu13891	NM_001135213.3 Latest version!	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$482.30 $689.00
OHu13920	NM_001135214.3 Latest version!	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$517.30 $739.00
OHu14013	NM_014776.5 Latest version!	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$482.30 $689.00
OHu46998	NM_001330153.2 Latest version!	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu46994	XM_005253997.4 Latest version!	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu46995	XM_006719707.4 Latest version!	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu46996	XM_006719708.4 Latest version!	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu46997	XM_006719709.4 Latest version!	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu84344	XM_017020258.2 Latest version!	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu84345	XM_017020259.2 Latest version!	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu84346	XM_017020260.2 Latest version!	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu19439	NM_057169.3	Homo sapiens G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$384.50-$538.30 $769.00
OHu84344	XM_017020258.1	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu46997	XM_006719709.3	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu47001	XM_006719712.3	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu13920	NM_001135214.2	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$517.30 $739.00
OHu47003	NM_001330154.1	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu19439	NM_057169.4	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$384.50-$538.30 $769.00
OHu84346	XM_017020260.1	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu84347	XM_017020261.1	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu84345	XM_017020259.1	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu13891	NM_001135213.2	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$482.30 $689.00
OHu13926	NM_057170.3	Homo sapiens G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu13992	NM_139201.2	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu47002	XM_006719713.3	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu46995	XM_006719707.3	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu46994	XM_005253997.3	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu13926	NM_057170.4	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu14013	NM_014776.4	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$482.30 $689.00
OHu46998	NM_001330153.1	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu46996	XM_006719708.3	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GIT2 ( NM_057169.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_476510.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19439
Clone ID Related Accession (Same CDS sequence)	NM_057169.4 , NM_057169.3 , NM_057169.5
Accession Version	NM_057169.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-09
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP871813.1, AF124491.1, D63482.2 and AA813937.1. This sequence is a reference standard in the RefSeqGene project. On Aug 18, 2016 this sequence version replaced NM_057169.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.234002.1, SRR1803612.143685.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT 
ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA 
ACCAATGTAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC 
TTAAAGAGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACCGGATCA 
GGTCAGAAAC CATATCTCCC AATGGGAGAA GCGAGCCGCC CCGAAGAGAG CAGGATGAGA 
CTCCAGCCCT TCCCCGCGCA CATCGGGAGG AGTGCACTTG TGACCTCCTC TTCATCTCTG 
CCTTCCTTCC CCTCCACACT TTCCTGGTCG AGGGACGAAA GCGCCCGAAG GGCATCCAGG 
CTGGAGAAGC AGAACAGCAC ACCTGAGAGT GACTACGACA ACACTCCCAA CGACATGGAG 
CCAGATGGCA TGGGGTCAAG CCGAAAGGGA CGGCAAAGAA GTATGGTGTG GCCAGGGGAT 
GGCTTGGTAC CAGACACAGC AGAACCCCAT GTGGCCCCAA GCCCCACTCT CCCTAGCACC 
GAAGATGTCA TCAGGAAGAC TGAACAGATC ACCAAAAACA TACAGGAGCT CTTAAGAGCA 
GCCCAAGAAA ATAAACATGA CAGTTATATT CCCTGCTCAG AGAGGATACA CGTAGCTGTT 
ACAGAAATGG CAGCATTATT CCCCAAAAAA CCCAAGTCTG ATATGGTGAG GACTTCCCTT 
CGTTTACTGA CGTCCAGTGC CTACCGACTG CAGTCAGAGT GCAAGAAGAC CCTCCCAGGG 
GACCCCGGCT CACCCACAGA CGTTCAGCTG GTCACGCAGC AGGTCATCCA GTGTGCGTAC 
GACATCGCCA AGGCTGCCAA GCAGCTGGTT ACCATCACCA CCAAAGAGAA CAACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476510.1
CDS	166..2445
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKVNNNLSDELRIMQKKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHIGRSALVTSSSSLPSFPSTLSWSRDESARRASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Target ORF information:

RefSeq Version	NM_057169.4
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057169.4

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT 
ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA 
ACCAATGTAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC 
TTAAAGAGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACCGGATCA 
GGTCAGAAAC CATATCTCCC AATGGGAGAA GCGAGCCGCC CCGAAGAGAG CAGGATGAGA 
CTCCAGCCCT TCCCCGCGCA CATCGGGAGG AGTGCACTTG TGACCTCCTC TTCATCTCTG 
CCTTCCTTCC CCTCCACACT TTCCTGGTCG AGGGACGAAA GCGCCCGAAG GGCATCCAGG 
CTGGAGAAGC AGAACAGCAC ACCTGAGAGT GACTACGACA ACACTCCCAA CGACATGGAG 
CCAGATGGCA TGGGGTCAAG CCGAAAGGGA CGGCAAAGAA GTATGGTGTG GCCAGGGGAT 
GGCTTGGTAC CAGACACAGC AGAACCCCAT GTGGCCCCAA GCCCCACTCT CCCTAGCACC 
GAAGATGTCA TCAGGAAGAC TGAACAGATC ACCAAAAACA TACAGGAGCT CTTAAGAGCA 
GCCCAAGAAA ATAAACATGA CAGTTATATT CCCTGCTCAG AGAGGATACA CGTAGCTGTT 
ACAGAAATGG CAGCATTATT CCCCAAAAAA CCCAAGTCTG ATATGGTGAG GACTTCCCTT 
CGTTTACTGA CGTCCAGTGC CTACCGACTG CAGTCAGAGT GCAAGAAGAC CCTCCCAGGG 
GACCCCGGCT CACCCACAGA CGTTCAGCTG GTCACGCAGC AGGTCATCCA GTGTGCGTAC 
GACATCGCCA AGGCTGCCAA GCAGCTGGTT ACCATCACCA CCAAAGAGAA CAACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( NM_057169.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_476510.1 Homo sapiens G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 1, mRNA.

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CloneID	OHu19439
Clone ID Related Accession (Same CDS sequence)	NM_057169.4 , NM_057169.3 , NM_057169.5
Accession Version	NM_057169.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	ARF GTPase-activating protein GIT2 isoform 1
Comment

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT 
ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA 
ACCAATGTAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC 
TTAAAGAGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACCGGATCA 
GGTCAGAAAC CATATCTCCC AATGGGAGAA GCGAGCCGCC CCGAAGAGAG CAGGATGAGA 
CTCCAGCCCT TCCCCGCGCA CATCGGGAGG AGTGCACTTG TGACCTCCTC TTCATCTCTG 
CCTTCCTTCC CCTCCACACT TTCCTGGTCG AGGGACGAAA GCGCCCGAAG GGCATCCAGG 
CTGGAGAAGC AGAACAGCAC ACCTGAGAGT GACTACGACA ACACTCCCAA CGACATGGAG 
CCAGATGGCA TGGGGTCAAG CCGAAAGGGA CGGCAAAGAA GTATGGTGTG GCCAGGGGAT 
GGCTTGGTAC CAGACACAGC AGAACCCCAT GTGGCCCCAA GCCCCACTCT CCCTAGCACC 
GAAGATGTCA TCAGGAAGAC TGAACAGATC ACCAAAAACA TACAGGAGCT CTTAAGAGCA 
GCCCAAGAAA ATAAACATGA CAGTTATATT CCCTGCTCAG AGAGGATACA CGTAGCTGTT 
ACAGAAATGG CAGCATTATT CCCCAAAAAA CCCAAGTCTG ATATGGTGAG GACTTCCCTT 
CGTTTACTGA CGTCCAGTGC CTACCGACTG CAGTCAGAGT GCAAGAAGAC CCTCCCAGGG 
GACCCCGGCT CACCCACAGA CGTTCAGCTG GTCACGCAGC AGGTCATCCA GTGTGCGTAC 
GACATCGCCA AGGCTGCCAA GCAGCTGGTT ACCATCACCA CCAAAGAGAA CAACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_476510.1

Translation

MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKVNNNLSDELRIMQKKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHIGRSALVTSSSSLPSFPSTLSWSRDESARRASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Target ORF information:

RefSeq Version	NM_057169.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057169.3

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT 
ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA 
ACCAATGTAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC 
TTAAAGAGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACCGGATCA 
GGTCAGAAAC CATATCTCCC AATGGGAGAA GCGAGCCGCC CCGAAGAGAG CAGGATGAGA 
CTCCAGCCCT TCCCCGCGCA CATCGGGAGG AGTGCACTTG TGACCTCCTC TTCATCTCTG 
CCTTCCTTCC CCTCCACACT TTCCTGGTCG AGGGACGAAA GCGCCCGAAG GGCATCCAGG 
CTGGAGAAGC AGAACAGCAC ACCTGAGAGT GACTACGACA ACACTCCCAA CGACATGGAG 
CCAGATGGCA TGGGGTCAAG CCGAAAGGGA CGGCAAAGAA GTATGGTGTG GCCAGGGGAT 
GGCTTGGTAC CAGACACAGC AGAACCCCAT GTGGCCCCAA GCCCCACTCT CCCTAGCACC 
GAAGATGTCA TCAGGAAGAC TGAACAGATC ACCAAAAACA TACAGGAGCT CTTAAGAGCA 
GCCCAAGAAA ATAAACATGA CAGTTATATT CCCTGCTCAG AGAGGATACA CGTAGCTGTT 
ACAGAAATGG CAGCATTATT CCCCAAAAAA CCCAAGTCTG ATATGGTGAG GACTTCCCTT 
CGTTTACTGA CGTCCAGTGC CTACCGACTG CAGTCAGAGT GCAAGAAGAC CCTCCCAGGG 
GACCCCGGCT CACCCACAGA CGTTCAGCTG GTCACGCAGC AGGTCATCCA GTGTGCGTAC 
GACATCGCCA AGGCTGCCAA GCAGCTGGTT ACCATCACCA CCAAAGAGAA CAACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( NM_057169.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_476510.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 1, mRNA.

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CloneID	OHu19439
Clone ID Related Accession (Same CDS sequence)	NM_057169.4 , NM_057169.3 , NM_057169.5
Accession Version	NM_057169.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP871813.1, AF124491.1, D63482.2 and AA813937.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_057169.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.234002.1, SRR1803617.110006.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000355312.8/ ENSP00000347464.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT 
ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA 
ACCAATGTAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC 
TTAAAGAGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACCGGATCA 
GGTCAGAAAC CATATCTCCC AATGGGAGAA GCGAGCCGCC CCGAAGAGAG CAGGATGAGA 
CTCCAGCCCT TCCCCGCGCA CATCGGGAGG AGTGCACTTG TGACCTCCTC TTCATCTCTG 
CCTTCCTTCC CCTCCACACT TTCCTGGTCG AGGGACGAAA GCGCCCGAAG GGCATCCAGG 
CTGGAGAAGC AGAACAGCAC ACCTGAGAGT GACTACGACA ACACTCCCAA CGACATGGAG 
CCAGATGGCA TGGGGTCAAG CCGAAAGGGA CGGCAAAGAA GTATGGTGTG GCCAGGGGAT 
GGCTTGGTAC CAGACACAGC AGAACCCCAT GTGGCCCCAA GCCCCACTCT CCCTAGCACC 
GAAGATGTCA TCAGGAAGAC TGAACAGATC ACCAAAAACA TACAGGAGCT CTTAAGAGCA 
GCCCAAGAAA ATAAACATGA CAGTTATATT CCCTGCTCAG AGAGGATACA CGTAGCTGTT 
ACAGAAATGG CAGCATTATT CCCCAAAAAA CCCAAGTCTG ATATGGTGAG GACTTCCCTT 
CGTTTACTGA CGTCCAGTGC CTACCGACTG CAGTCAGAGT GCAAGAAGAC CCTCCCAGGG 
GACCCCGGCT CACCCACAGA CGTTCAGCTG GTCACGCAGC AGGTCATCCA GTGTGCGTAC 
GACATCGCCA AGGCTGCCAA GCAGCTGGTT ACCATCACCA CCAAAGAGAA CAACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476510.1
CDS	145..2424
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKVNNNLSDELRIMQKKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHIGRSALVTSSSSLPSFPSTLSWSRDESARRASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Target ORF information:

RefSeq Version	NM_057169.5
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057169.5

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT 
ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA 
ACCAATGTAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC 
TTAAAGAGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACCGGATCA 
GGTCAGAAAC CATATCTCCC AATGGGAGAA GCGAGCCGCC CCGAAGAGAG CAGGATGAGA 
CTCCAGCCCT TCCCCGCGCA CATCGGGAGG AGTGCACTTG TGACCTCCTC TTCATCTCTG 
CCTTCCTTCC CCTCCACACT TTCCTGGTCG AGGGACGAAA GCGCCCGAAG GGCATCCAGG 
CTGGAGAAGC AGAACAGCAC ACCTGAGAGT GACTACGACA ACACTCCCAA CGACATGGAG 
CCAGATGGCA TGGGGTCAAG CCGAAAGGGA CGGCAAAGAA GTATGGTGTG GCCAGGGGAT 
GGCTTGGTAC CAGACACAGC AGAACCCCAT GTGGCCCCAA GCCCCACTCT CCCTAGCACC 
GAAGATGTCA TCAGGAAGAC TGAACAGATC ACCAAAAACA TACAGGAGCT CTTAAGAGCA 
GCCCAAGAAA ATAAACATGA CAGTTATATT CCCTGCTCAG AGAGGATACA CGTAGCTGTT 
ACAGAAATGG CAGCATTATT CCCCAAAAAA CCCAAGTCTG ATATGGTGAG GACTTCCCTT 
CGTTTACTGA CGTCCAGTGC CTACCGACTG CAGTCAGAGT GCAAGAAGAC CCTCCCAGGG 
GACCCCGGCT CACCCACAGA CGTTCAGCTG GTCACGCAGC AGGTCATCCA GTGTGCGTAC 
GACATCGCCA AGGCTGCCAA GCAGCTGGTT ACCATCACCA CCAAAGAGAA CAACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( NM_057170.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_476511.1 Homo sapiens G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu13926
Clone ID Related Accession (Same CDS sequence)	NM_057170.3 , NM_057170.5 , NM_057170.4
Accession Version	NM_057170.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	ARF GTPase-activating protein GIT2 isoform 2
Comment

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGCA TCCAGGCTGG AGAAGCAGAA CAGCACACCT 
GAGAGTGACT ACGACAACAC TCCCAACGAC ATGGAGCCAG ATGGCATGGG GTCAAGCCGA 
AAGGGACGGC AAAGAAGTAT GGTGTGGCCA GGGGATGGCT TGGTACCAGA CACAGCAGAA 
CCCCATGTGG CCCCAAGCCC CACTCTCCCT AGCACCGAAG ATGTCATCAG GAAGACTGAA 
CAGATCACCA AAAACATACA GGAGCTCTTA AGAGCAGCCC AAGAAAATAA ACATGACAGT 
TATATTCCCT GCTCAGAGAG GATACACGTA GCTGTTACAG AAATGGCAGC ATTATTCCCC 
AAAAAACCCA AGTCTGATAT GGTGAGGACT TCCCTTCGTT TACTGACGTC CAGTGCCTAC 
CGACTGCAGT CAGAGTGCAA GAAGACCCTC CCAGGGGACC CCGGCTCACC CACAGACGTT 
CAGCTGGTCA CGCAGCAGGT CATCCAGTGT GCGTACGACA TCGCCAAGGC TGCCAAGCAG 
CTGGTTACCA TCACCACCAA AGAGAACAAC AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_476511.1

Translation

MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Target ORF information:

RefSeq Version	NM_057170.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057170.3

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGCA TCCAGGCTGG AGAAGCAGAA CAGCACACCT 
GAGAGTGACT ACGACAACAC TCCCAACGAC ATGGAGCCAG ATGGCATGGG GTCAAGCCGA 
AAGGGACGGC AAAGAAGTAT GGTGTGGCCA GGGGATGGCT TGGTACCAGA CACAGCAGAA 
CCCCATGTGG CCCCAAGCCC CACTCTCCCT AGCACCGAAG ATGTCATCAG GAAGACTGAA 
CAGATCACCA AAAACATACA GGAGCTCTTA AGAGCAGCCC AAGAAAATAA ACATGACAGT 
TATATTCCCT GCTCAGAGAG GATACACGTA GCTGTTACAG AAATGGCAGC ATTATTCCCC 
AAAAAACCCA AGTCTGATAT GGTGAGGACT TCCCTTCGTT TACTGACGTC CAGTGCCTAC 
CGACTGCAGT CAGAGTGCAA GAAGACCCTC CCAGGGGACC CCGGCTCACC CACAGACGTT 
CAGCTGGTCA CGCAGCAGGT CATCCAGTGT GCGTACGACA TCGCCAAGGC TGCCAAGCAG 
CTGGTTACCA TCACCACCAA AGAGAACAAC AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( NM_057170.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_476511.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 2, mRNA.

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CloneID	OHu13926
Clone ID Related Accession (Same CDS sequence)	NM_057170.3 , NM_057170.5 , NM_057170.4
Accession Version	NM_057170.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP871813.1, AK308304.1, BC014223.2, D63482.2 and AA813937.1. On May 31, 2019 this sequence version replaced NM_057170.4. Transcript Variant: This variant (2) lacks two exons and a portion of a third exon in the coding region, as compared to variant 1. The reading frame is maintained. Isoform 2 is missing internal 128 aa but has the same N- and C-termini as isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGCA TCCAGGCTGG AGAAGCAGAA CAGCACACCT 
GAGAGTGACT ACGACAACAC TCCCAACGAC ATGGAGCCAG ATGGCATGGG GTCAAGCCGA 
AAGGGACGGC AAAGAAGTAT GGTGTGGCCA GGGGATGGCT TGGTACCAGA CACAGCAGAA 
CCCCATGTGG CCCCAAGCCC CACTCTCCCT AGCACCGAAG ATGTCATCAG GAAGACTGAA 
CAGATCACCA AAAACATACA GGAGCTCTTA AGAGCAGCCC AAGAAAATAA ACATGACAGT 
TATATTCCCT GCTCAGAGAG GATACACGTA GCTGTTACAG AAATGGCAGC ATTATTCCCC 
AAAAAACCCA AGTCTGATAT GGTGAGGACT TCCCTTCGTT TACTGACGTC CAGTGCCTAC 
CGACTGCAGT CAGAGTGCAA GAAGACCCTC CCAGGGGACC CCGGCTCACC CACAGACGTT 
CAGCTGGTCA CGCAGCAGGT CATCCAGTGT GCGTACGACA TCGCCAAGGC TGCCAAGCAG 
CTGGTTACCA TCACCACCAA AGAGAACAAC AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476511.1
CDS	145..2040
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Target ORF information:

RefSeq Version	NM_057170.5
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057170.5

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGCA TCCAGGCTGG AGAAGCAGAA CAGCACACCT 
GAGAGTGACT ACGACAACAC TCCCAACGAC ATGGAGCCAG ATGGCATGGG GTCAAGCCGA 
AAGGGACGGC AAAGAAGTAT GGTGTGGCCA GGGGATGGCT TGGTACCAGA CACAGCAGAA 
CCCCATGTGG CCCCAAGCCC CACTCTCCCT AGCACCGAAG ATGTCATCAG GAAGACTGAA 
CAGATCACCA AAAACATACA GGAGCTCTTA AGAGCAGCCC AAGAAAATAA ACATGACAGT 
TATATTCCCT GCTCAGAGAG GATACACGTA GCTGTTACAG AAATGGCAGC ATTATTCCCC 
AAAAAACCCA AGTCTGATAT GGTGAGGACT TCCCTTCGTT TACTGACGTC CAGTGCCTAC 
CGACTGCAGT CAGAGTGCAA GAAGACCCTC CCAGGGGACC CCGGCTCACC CACAGACGTT 
CAGCTGGTCA CGCAGCAGGT CATCCAGTGT GCGTACGACA TCGCCAAGGC TGCCAAGCAG 
CTGGTTACCA TCACCACCAA AGAGAACAAC AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( NM_057170.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_476511.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu13926
Clone ID Related Accession (Same CDS sequence)	NM_057170.3 , NM_057170.5 , NM_057170.4
Accession Version	NM_057170.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP871813.1, AK308304.1, BC014223.2, D63482.2 and AA813937.1. On Aug 18, 2016 this sequence version replaced NM_057170.3. Transcript Variant: This variant (2) lacks two exons and a portion of a third exon in the coding region, as compared to variant 1. The reading frame is maintained. Isoform 2 is missing internal 128 aa but has the same N- and C-termini as isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGCA TCCAGGCTGG AGAAGCAGAA CAGCACACCT 
GAGAGTGACT ACGACAACAC TCCCAACGAC ATGGAGCCAG ATGGCATGGG GTCAAGCCGA 
AAGGGACGGC AAAGAAGTAT GGTGTGGCCA GGGGATGGCT TGGTACCAGA CACAGCAGAA 
CCCCATGTGG CCCCAAGCCC CACTCTCCCT AGCACCGAAG ATGTCATCAG GAAGACTGAA 
CAGATCACCA AAAACATACA GGAGCTCTTA AGAGCAGCCC AAGAAAATAA ACATGACAGT 
TATATTCCCT GCTCAGAGAG GATACACGTA GCTGTTACAG AAATGGCAGC ATTATTCCCC 
AAAAAACCCA AGTCTGATAT GGTGAGGACT TCCCTTCGTT TACTGACGTC CAGTGCCTAC 
CGACTGCAGT CAGAGTGCAA GAAGACCCTC CCAGGGGACC CCGGCTCACC CACAGACGTT 
CAGCTGGTCA CGCAGCAGGT CATCCAGTGT GCGTACGACA TCGCCAAGGC TGCCAAGCAG 
CTGGTTACCA TCACCACCAA AGAGAACAAC AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476511.1
CDS	166..2061
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Target ORF information:

RefSeq Version	NM_057170.4
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057170.4

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGCA TCCAGGCTGG AGAAGCAGAA CAGCACACCT 
GAGAGTGACT ACGACAACAC TCCCAACGAC ATGGAGCCAG ATGGCATGGG GTCAAGCCGA 
AAGGGACGGC AAAGAAGTAT GGTGTGGCCA GGGGATGGCT TGGTACCAGA CACAGCAGAA 
CCCCATGTGG CCCCAAGCCC CACTCTCCCT AGCACCGAAG ATGTCATCAG GAAGACTGAA 
CAGATCACCA AAAACATACA GGAGCTCTTA AGAGCAGCCC AAGAAAATAA ACATGACAGT 
TATATTCCCT GCTCAGAGAG GATACACGTA GCTGTTACAG AAATGGCAGC ATTATTCCCC 
AAAAAACCCA AGTCTGATAT GGTGAGGACT TCCCTTCGTT TACTGACGTC CAGTGCCTAC 
CGACTGCAGT CAGAGTGCAA GAAGACCCTC CCAGGGGACC CCGGCTCACC CACAGACGTT 
CAGCTGGTCA CGCAGCAGGT CATCCAGTGT GCGTACGACA TCGCCAAGGC TGCCAAGCAG 
CTGGTTACCA TCACCACCAA AGAGAACAAC AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( NM_139201.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_631940.2 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu13992
Clone ID Related Accession (Same CDS sequence)	NM_139201.3 , NM_139201.2
Accession Version	NM_139201.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP871813.1, AK222736.1, BM557270.1 and AC084876.19. On May 31, 2019 this sequence version replaced NM_139201.2. Transcript Variant: This variant (4) lacks most 3' exons and has an alternate exon at the 3' end, as compared to variant 1. Isoform 4 is much shorter and has a different C-terminus than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.282861.1, AK222736.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT 
ATGCAGAAAA AGTTGCTTGG AAAAGATGCT AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_631940.2
CDS	145..1560
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKVNNNLSDELRIMQKKLLGKDAN

Target ORF information:

RefSeq Version	NM_139201.3
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139201.3

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT 
ATGCAGAAAA AGTTGCTTGG AAAAGATGCT AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( NM_139201.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_631940.2 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu13992
Clone ID Related Accession (Same CDS sequence)	NM_139201.3 , NM_139201.2
Accession Version	NM_139201.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP871813.1, AK222736.1 and BM557270.1. On Sep 26, 2008 this sequence version replaced NM_139201.1. Transcript Variant: This variant (4) lacks most 3' exons and has an alternate exon at the 3' end, as compared to variant 1. Isoform 4 is much shorter and has a different C-terminus than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK222736.1, SRR1163657.308011.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT 
ATGCAGAAAA AGTTGCTTGG AAAAGATGCT AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_631940.2
CDS	166..1581
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKVNNNLSDELRIMQKKLLGKDAN

Target ORF information:

RefSeq Version	NM_139201.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139201.2

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT 
ATGCAGAAAA AGTTGCTTGG AAAAGATGCT AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( NM_001330154.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317083.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu47003
Clone ID Related Accession (Same CDS sequence)	NM_001330154.1 , NM_001330154.2
Accession Version	NM_001330154.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP871813.1, AK222736.1 and BM557270.1. On Aug 18, 2016 this sequence version replaced XM_006719714.3. Transcript Variant: This variant (8) lacks most 3' exons and has an alternate exon at the 3' end, as compared to variant 1. Isoform 8 is much shorter and has a different C-terminus than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.209549.1, SRR1803613.83957.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGTTGCTTGG AAAAGATGCT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317083.1
CDS	166..1431
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKLLGKDAN

Target ORF information:

RefSeq Version	NM_001330154.1
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330154.1

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGTTGCTTGG AAAAGATGCT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( NM_001330154.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317083.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu47003
Clone ID Related Accession (Same CDS sequence)	NM_001330154.1 , NM_001330154.2
Accession Version	NM_001330154.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP871813.1, AK222736.1, BM557270.1 and AC084876.19. On May 31, 2019 this sequence version replaced NM_001330154.1. Transcript Variant: This variant (8) lacks most 3' exons and has an alternate exon at the 3' end, as compared to variant 1. Isoform 8 is much shorter and has a different C-terminus than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.209549.1, SRR1803613.83957.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGTTGCTTGG AAAAGATGCT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317083.1
CDS	145..1410
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKLLGKDAN

Target ORF information:

RefSeq Version	NM_001330154.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330154.2

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGTTGCTTGG AAAAGATGCT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( XM_006719712.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719775.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu47001
Clone ID Related Accession (Same CDS sequence)	XM_006719712.4 , XM_006719712.3
Accession Version	XM_006719712.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006719712.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGGCATCCAG GCTGGAGAAG 
CAGAACAGCA CACCTGAGAG TGACTACGAC AACACTCCCA ACGACATGGA GCCAGATGGC 
ATGGGGTCAA GCCGAAAGGG ACGGCAAAGA AGTATGGTGT GGCCAGGGGA TGGCTTGGTA 
CCAGACACAG CAGAACCCCA TGTGGCCCCA AGCCCCACTC TCCCTAGCAC CGAAGATGTC 
ATCAGGAAGA CTGAACAGAT CACCAAAAAC ATACAGGAGC TCTTAAGAGC AGCCCAAGAA 
AATAAACATG ACAGTTATAT TCCCTGCTCA GAGAGGATAC ACGTAGCTGT TACAGAAATG 
GCAGCATTAT TCCCCAAAAA ACCCAAGTCT GATATGGTGA GGACTTCCCT TCGTTTACTG 
ACGTCCAGTG CCTACCGACT GCAGTCAGAG TGCAAGAAGA CCCTCCCAGG GGACCCCGGC 
TCACCCACAG ACGTTCAGCT GGTCACGCAG CAGGTCATCC AGTGTGCGTA CGACATCGCC 
AAGGCTGCCA AGCAGCTGGT TACCATCACC ACCAAAGAGA ACAACAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719775.1
CDS	71..1861
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Target ORF information:

RefSeq Version	XM_006719712.4
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006719712.4

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGGCATCCAG GCTGGAGAAG 
CAGAACAGCA CACCTGAGAG TGACTACGAC AACACTCCCA ACGACATGGA GCCAGATGGC 
ATGGGGTCAA GCCGAAAGGG ACGGCAAAGA AGTATGGTGT GGCCAGGGGA TGGCTTGGTA 
CCAGACACAG CAGAACCCCA TGTGGCCCCA AGCCCCACTC TCCCTAGCAC CGAAGATGTC 
ATCAGGAAGA CTGAACAGAT CACCAAAAAC ATACAGGAGC TCTTAAGAGC AGCCCAAGAA 
AATAAACATG ACAGTTATAT TCCCTGCTCA GAGAGGATAC ACGTAGCTGT TACAGAAATG 
GCAGCATTAT TCCCCAAAAA ACCCAAGTCT GATATGGTGA GGACTTCCCT TCGTTTACTG 
ACGTCCAGTG CCTACCGACT GCAGTCAGAG TGCAAGAAGA CCCTCCCAGG GGACCCCGGC 
TCACCCACAG ACGTTCAGCT GGTCACGCAG CAGGTCATCC AGTGTGCGTA CGACATCGCC 
AAGGCTGCCA AGCAGCTGGT TACCATCACC ACCAAAGAGA ACAACAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( XM_006719712.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719775.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu47001
Clone ID Related Accession (Same CDS sequence)	XM_006719712.4 , XM_006719712.3
Accession Version	XM_006719712.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006719712.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGGCATCCAG GCTGGAGAAG 
CAGAACAGCA CACCTGAGAG TGACTACGAC AACACTCCCA ACGACATGGA GCCAGATGGC 
ATGGGGTCAA GCCGAAAGGG ACGGCAAAGA AGTATGGTGT GGCCAGGGGA TGGCTTGGTA 
CCAGACACAG CAGAACCCCA TGTGGCCCCA AGCCCCACTC TCCCTAGCAC CGAAGATGTC 
ATCAGGAAGA CTGAACAGAT CACCAAAAAC ATACAGGAGC TCTTAAGAGC AGCCCAAGAA 
AATAAACATG ACAGTTATAT TCCCTGCTCA GAGAGGATAC ACGTAGCTGT TACAGAAATG 
GCAGCATTAT TCCCCAAAAA ACCCAAGTCT GATATGGTGA GGACTTCCCT TCGTTTACTG 
ACGTCCAGTG CCTACCGACT GCAGTCAGAG TGCAAGAAGA CCCTCCCAGG GGACCCCGGC 
TCACCCACAG ACGTTCAGCT GGTCACGCAG CAGGTCATCC AGTGTGCGTA CGACATCGCC 
AAGGCTGCCA AGCAGCTGGT TACCATCACC ACCAAAGAGA ACAACAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719775.1
CDS	161..1951
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Target ORF information:

RefSeq Version	XM_006719712.3
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006719712.3

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGGCATCCAG GCTGGAGAAG 
CAGAACAGCA CACCTGAGAG TGACTACGAC AACACTCCCA ACGACATGGA GCCAGATGGC 
ATGGGGTCAA GCCGAAAGGG ACGGCAAAGA AGTATGGTGT GGCCAGGGGA TGGCTTGGTA 
CCAGACACAG CAGAACCCCA TGTGGCCCCA AGCCCCACTC TCCCTAGCAC CGAAGATGTC 
ATCAGGAAGA CTGAACAGAT CACCAAAAAC ATACAGGAGC TCTTAAGAGC AGCCCAAGAA 
AATAAACATG ACAGTTATAT TCCCTGCTCA GAGAGGATAC ACGTAGCTGT TACAGAAATG 
GCAGCATTAT TCCCCAAAAA ACCCAAGTCT GATATGGTGA GGACTTCCCT TCGTTTACTG 
ACGTCCAGTG CCTACCGACT GCAGTCAGAG TGCAAGAAGA CCCTCCCAGG GGACCCCGGC 
TCACCCACAG ACGTTCAGCT GGTCACGCAG CAGGTCATCC AGTGTGCGTA CGACATCGCC 
AAGGCTGCCA AGCAGCTGGT TACCATCACC ACCAAAGAGA ACAACAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( XM_006719713.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719776.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu47002
Clone ID Related Accession (Same CDS sequence)	XM_006719713.4 , XM_006719713.3
Accession Version	XM_006719713.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006719713.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGTTG CTTGGAAAAG ATGCTAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719776.1
CDS	71..1441
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKLLGKDAN

Target ORF information:

RefSeq Version	XM_006719713.4
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006719713.4

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGTTG CTTGGAAAAG ATGCTAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( XM_006719713.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719776.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu47002
Clone ID Related Accession (Same CDS sequence)	XM_006719713.4 , XM_006719713.3
Accession Version	XM_006719713.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006719713.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGTTG CTTGGAAAAG ATGCTAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719776.1
CDS	158..1528
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKLLGKDAN

Target ORF information:

RefSeq Version	XM_006719713.3
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006719713.3

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGTTG CTTGGAAAAG ATGCTAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( XM_017020261.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875750.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu84347
Clone ID Related Accession (Same CDS sequence)	XM_017020261.2 , XM_017020261.1
Accession Version	XM_017020261.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017020261.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCCTGGA TTTGTCTGAA 
TTGGCAAAAG CTGCTAAGAA GAAACTTCAA TCTCTAAGTA ATCATTTGTT TGAAGAACTT 
GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACGG ATGCAGTCTG GCTTGCCACG 
CAAAACCACA GCGCCCTGGT AACCGAGACA ACGGTCGTCC CCTTTCTTCC GGTCAATCCT 
GAGTACTCAT CAACACGAAA TCAGGGCAGA CAGAAGTTAG CTCGGTTCAA CGCCCATGAG 
TTTGCCACGC TGGTCATTGA CATTCTCAGT GACGCCAAGA GGAGACAGCA GGGCAGTTCT 
CTCTCGGGTT CAAAAGACAA TGTGGAGCTC ATACTGAAAA CCATCAATAA CCAGCACAGC 
GTTGAGAGTC AAGACAACGA TCAGCCCGAC TATGACAGCG TGGCATCAGA CGAAGACACA 
GATTTGGAAA CCACTGCAAG CAAAACAAAC CGGCAGAAGA GCCTAGATTC AGATTTATCA 
GATGGACCAG TCACTGTACA GGAATTTATG GAGGTCAAAA ACGCTCTAGT GGCTTCTGAG 
GCCAAGATAC AGCAGCTAAT GAAGGTGAAT AACAACTTGA GTGACGAGCT GAGAATTATG 
CAGAAAAAGT TGCTTGGAAA AGATGCTAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875750.1
CDS	71..1483
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKVNNNLSDELRIMQKKLLGKDAN

Target ORF information:

RefSeq Version	XM_017020261.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020261.2

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCCTGGA TTTGTCTGAA 
TTGGCAAAAG CTGCTAAGAA GAAACTTCAA TCTCTAAGTA ATCATTTGTT TGAAGAACTT 
GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACGG ATGCAGTCTG GCTTGCCACG 
CAAAACCACA GCGCCCTGGT AACCGAGACA ACGGTCGTCC CCTTTCTTCC GGTCAATCCT 
GAGTACTCAT CAACACGAAA TCAGGGCAGA CAGAAGTTAG CTCGGTTCAA CGCCCATGAG 
TTTGCCACGC TGGTCATTGA CATTCTCAGT GACGCCAAGA GGAGACAGCA GGGCAGTTCT 
CTCTCGGGTT CAAAAGACAA TGTGGAGCTC ATACTGAAAA CCATCAATAA CCAGCACAGC 
GTTGAGAGTC AAGACAACGA TCAGCCCGAC TATGACAGCG TGGCATCAGA CGAAGACACA 
GATTTGGAAA CCACTGCAAG CAAAACAAAC CGGCAGAAGA GCCTAGATTC AGATTTATCA 
GATGGACCAG TCACTGTACA GGAATTTATG GAGGTCAAAA ACGCTCTAGT GGCTTCTGAG 
GCCAAGATAC AGCAGCTAAT GAAGGTGAAT AACAACTTGA GTGACGAGCT GAGAATTATG 
CAGAAAAAGT TGCTTGGAAA AGATGCTAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( XM_017020261.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875750.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu84347
Clone ID Related Accession (Same CDS sequence)	XM_017020261.2 , XM_017020261.1
Accession Version	XM_017020261.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCCTGGA TTTGTCTGAA 
TTGGCAAAAG CTGCTAAGAA GAAACTTCAA TCTCTAAGTA ATCATTTGTT TGAAGAACTT 
GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACGG ATGCAGTCTG GCTTGCCACG 
CAAAACCACA GCGCCCTGGT AACCGAGACA ACGGTCGTCC CCTTTCTTCC GGTCAATCCT 
GAGTACTCAT CAACACGAAA TCAGGGCAGA CAGAAGTTAG CTCGGTTCAA CGCCCATGAG 
TTTGCCACGC TGGTCATTGA CATTCTCAGT GACGCCAAGA GGAGACAGCA GGGCAGTTCT 
CTCTCGGGTT CAAAAGACAA TGTGGAGCTC ATACTGAAAA CCATCAATAA CCAGCACAGC 
GTTGAGAGTC AAGACAACGA TCAGCCCGAC TATGACAGCG TGGCATCAGA CGAAGACACA 
GATTTGGAAA CCACTGCAAG CAAAACAAAC CGGCAGAAGA GCCTAGATTC AGATTTATCA 
GATGGACCAG TCACTGTACA GGAATTTATG GAGGTCAAAA ACGCTCTAGT GGCTTCTGAG 
GCCAAGATAC AGCAGCTAAT GAAGGTGAAT AACAACTTGA GTGACGAGCT GAGAATTATG 
CAGAAAAAGT TGCTTGGAAA AGATGCTAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875750.1
CDS	156..1568
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKVNNNLSDELRIMQKKLLGKDAN

Target ORF information:

RefSeq Version	XM_017020261.1
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020261.1

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCCTGGA TTTGTCTGAA 
TTGGCAAAAG CTGCTAAGAA GAAACTTCAA TCTCTAAGTA ATCATTTGTT TGAAGAACTT 
GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACGG ATGCAGTCTG GCTTGCCACG 
CAAAACCACA GCGCCCTGGT AACCGAGACA ACGGTCGTCC CCTTTCTTCC GGTCAATCCT 
GAGTACTCAT CAACACGAAA TCAGGGCAGA CAGAAGTTAG CTCGGTTCAA CGCCCATGAG 
TTTGCCACGC TGGTCATTGA CATTCTCAGT GACGCCAAGA GGAGACAGCA GGGCAGTTCT 
CTCTCGGGTT CAAAAGACAA TGTGGAGCTC ATACTGAAAA CCATCAATAA CCAGCACAGC 
GTTGAGAGTC AAGACAACGA TCAGCCCGAC TATGACAGCG TGGCATCAGA CGAAGACACA 
GATTTGGAAA CCACTGCAAG CAAAACAAAC CGGCAGAAGA GCCTAGATTC AGATTTATCA 
GATGGACCAG TCACTGTACA GGAATTTATG GAGGTCAAAA ACGCTCTAGT GGCTTCTGAG 
GCCAAGATAC AGCAGCTAAT GAAGGTGAAT AACAACTTGA GTGACGAGCT GAGAATTATG 
CAGAAAAAGT TGCTTGGAAA AGATGCTAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( NM_001135213.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001128685.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 6, mRNA.

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CloneID	OHu13891
Clone ID Related Accession (Same CDS sequence)	NM_001135213.3 , NM_001135213.2
Accession Version	NM_001135213.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP871813.1, D63482.2 and AA813937.1. On May 31, 2019 this sequence version replaced NM_001135213.2. Transcript Variant: This variant (6) lacks two in-frame exons in the 3' coding region and includes an additional short in-frame exon in the central coding region, compared to isoform 1. The resulting isoform (6) is missing two internal fragments and includes a 2 residue insertion, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D63482.2, SRR1803616.146694.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT 
TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTTCAAT CTCTAAGTAA TCATTTGTTT 
GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACGGA TGCAGTCTGG 
CTTGCCACGC AAAACCACAG CGCCCTGGTA ACCGAGACAA CGGTCGTCCC CTTTCTTCCG 
GTCAATCCTG AGTACTCATC AACACGAAAT CAGGGCAGAC AGAAGTTAGC TCGGTTCAAC 
GCCCATGAGT TTGCCACGCT GGTCATTGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAG 
GGCAGTTCTC TCTCGGGTTC AAAAGACAAT GTGGAGCTCA TACTGAAAAC CATCAATAAC 
CAGCACAGCG TTGAGAGTCA AGACAACGAT CAGCCCGACT ATGACAGCGT GGCATCAGAC 
GAAGACACAG ATTTGGAAAC CACTGCAAGC AAAACAAACC GGCAGAAGCT TCAAACACTC 
CAGAGTGAAA ATTCGAACCT CAGGAAACAG GCCACAACCA ATGTATATCA GGTGCAAACT 
GGTTCTGAGT ACACAGACAC TTCCAACCAC TCTTCCTTAA AGAGACGTCC GTCTGCCCGG 
GGCAGTAGGC CCATGTCCAT GTACGAGACC GGATCAGGTC AGAAACCATA TCTCCCAATG 
GGAGAAGCGA GCCGCCCCGA AGAGAGCAGG ATGAGACTCC AGCCCTTCCC CGCGCACGCA 
TCCAGGCTGG AGAAGCAGAA CAGCACACCT GAGAGTGACT ACGACAACAC TCCCAACGAC 
ATGGAGCCAG ATGGCATGGG GTCAAGCCGA AAGGGACGGC AAAGAAGTAT GGTGTGGCCA 
GGGGATGGCT TGGTACCAGA CACAGCAGAA CCCCATGTGG CCCCAAGCCC CACTCTCCCT 
AGCACCGAAG ATGTCATCAG GAAGACTGAA CAGATCACCA AAAACATACA GGAGCTCTTA 
AGAGCAGCCC AAGAAAATAA ACATGACAGT TATATTCCCT GCTCAGAGAG GATACACGTA 
GCTGTTACAG AAATGGCAGC ATTATTCCCC AAAAAACCCA AGTCTGATAT GGTGAGGACT 
TCCCTTCGTT TACTGACGTC CAGTGCCTAC CGACTGCAGT CAGAGTGCAA GAAGACCCTC 
CCAGGGGACC CCGGCTCACC CACAGACGTT CAGCTGGTCA CGCAGCAGGT CATCCAGTGT 
GCGTACGACA TCGCCAAGGC TGCCAAGCAG CTGGTTACCA TCACCACCAA AGAGAACAAC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128685.1
CDS	145..2190
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADRRLSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Target ORF information:

RefSeq Version	NM_001135213.3
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135213.3

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT 
TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTTCAAT CTCTAAGTAA TCATTTGTTT 
GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACGGA TGCAGTCTGG 
CTTGCCACGC AAAACCACAG CGCCCTGGTA ACCGAGACAA CGGTCGTCCC CTTTCTTCCG 
GTCAATCCTG AGTACTCATC AACACGAAAT CAGGGCAGAC AGAAGTTAGC TCGGTTCAAC 
GCCCATGAGT TTGCCACGCT GGTCATTGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAG 
GGCAGTTCTC TCTCGGGTTC AAAAGACAAT GTGGAGCTCA TACTGAAAAC CATCAATAAC 
CAGCACAGCG TTGAGAGTCA AGACAACGAT CAGCCCGACT ATGACAGCGT GGCATCAGAC 
GAAGACACAG ATTTGGAAAC CACTGCAAGC AAAACAAACC GGCAGAAGCT TCAAACACTC 
CAGAGTGAAA ATTCGAACCT CAGGAAACAG GCCACAACCA ATGTATATCA GGTGCAAACT 
GGTTCTGAGT ACACAGACAC TTCCAACCAC TCTTCCTTAA AGAGACGTCC GTCTGCCCGG 
GGCAGTAGGC CCATGTCCAT GTACGAGACC GGATCAGGTC AGAAACCATA TCTCCCAATG 
GGAGAAGCGA GCCGCCCCGA AGAGAGCAGG ATGAGACTCC AGCCCTTCCC CGCGCACGCA 
TCCAGGCTGG AGAAGCAGAA CAGCACACCT GAGAGTGACT ACGACAACAC TCCCAACGAC 
ATGGAGCCAG ATGGCATGGG GTCAAGCCGA AAGGGACGGC AAAGAAGTAT GGTGTGGCCA 
GGGGATGGCT TGGTACCAGA CACAGCAGAA CCCCATGTGG CCCCAAGCCC CACTCTCCCT 
AGCACCGAAG ATGTCATCAG GAAGACTGAA CAGATCACCA AAAACATACA GGAGCTCTTA 
AGAGCAGCCC AAGAAAATAA ACATGACAGT TATATTCCCT GCTCAGAGAG GATACACGTA 
GCTGTTACAG AAATGGCAGC ATTATTCCCC AAAAAACCCA AGTCTGATAT GGTGAGGACT 
TCCCTTCGTT TACTGACGTC CAGTGCCTAC CGACTGCAGT CAGAGTGCAA GAAGACCCTC 
CCAGGGGACC CCGGCTCACC CACAGACGTT CAGCTGGTCA CGCAGCAGGT CATCCAGTGT 
GCGTACGACA TCGCCAAGGC TGCCAAGCAG CTGGTTACCA TCACCACCAA AGAGAACAAC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( NM_001135213.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001128685.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu13891
Clone ID Related Accession (Same CDS sequence)	NM_001135213.3 , NM_001135213.2
Accession Version	NM_001135213.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP871813.1, D63482.2 and AA813937.1. On Aug 18, 2016 this sequence version replaced NM_001135213.1. Transcript Variant: This variant (6) lacks two in-frame exons in the 3' coding region and includes an additional short in-frame exon in the central coding region, compared to isoform 1. The resulting isoform (6) is missing two internal fragments and includes a 2 residue insertion, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.44149.1, SRR1803612.110304.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT 
TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTTCAAT CTCTAAGTAA TCATTTGTTT 
GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACGGA TGCAGTCTGG 
CTTGCCACGC AAAACCACAG CGCCCTGGTA ACCGAGACAA CGGTCGTCCC CTTTCTTCCG 
GTCAATCCTG AGTACTCATC AACACGAAAT CAGGGCAGAC AGAAGTTAGC TCGGTTCAAC 
GCCCATGAGT TTGCCACGCT GGTCATTGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAG 
GGCAGTTCTC TCTCGGGTTC AAAAGACAAT GTGGAGCTCA TACTGAAAAC CATCAATAAC 
CAGCACAGCG TTGAGAGTCA AGACAACGAT CAGCCCGACT ATGACAGCGT GGCATCAGAC 
GAAGACACAG ATTTGGAAAC CACTGCAAGC AAAACAAACC GGCAGAAGCT TCAAACACTC 
CAGAGTGAAA ATTCGAACCT CAGGAAACAG GCCACAACCA ATGTATATCA GGTGCAAACT 
GGTTCTGAGT ACACAGACAC TTCCAACCAC TCTTCCTTAA AGAGACGTCC GTCTGCCCGG 
GGCAGTAGGC CCATGTCCAT GTACGAGACC GGATCAGGTC AGAAACCATA TCTCCCAATG 
GGAGAAGCGA GCCGCCCCGA AGAGAGCAGG ATGAGACTCC AGCCCTTCCC CGCGCACGCA 
TCCAGGCTGG AGAAGCAGAA CAGCACACCT GAGAGTGACT ACGACAACAC TCCCAACGAC 
ATGGAGCCAG ATGGCATGGG GTCAAGCCGA AAGGGACGGC AAAGAAGTAT GGTGTGGCCA 
GGGGATGGCT TGGTACCAGA CACAGCAGAA CCCCATGTGG CCCCAAGCCC CACTCTCCCT 
AGCACCGAAG ATGTCATCAG GAAGACTGAA CAGATCACCA AAAACATACA GGAGCTCTTA 
AGAGCAGCCC AAGAAAATAA ACATGACAGT TATATTCCCT GCTCAGAGAG GATACACGTA 
GCTGTTACAG AAATGGCAGC ATTATTCCCC AAAAAACCCA AGTCTGATAT GGTGAGGACT 
TCCCTTCGTT TACTGACGTC CAGTGCCTAC CGACTGCAGT CAGAGTGCAA GAAGACCCTC 
CCAGGGGACC CCGGCTCACC CACAGACGTT CAGCTGGTCA CGCAGCAGGT CATCCAGTGT 
GCGTACGACA TCGCCAAGGC TGCCAAGCAG CTGGTTACCA TCACCACCAA AGAGAACAAC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128685.1
CDS	166..2211
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADRRLSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Target ORF information:

RefSeq Version	NM_001135213.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135213.2

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT 
TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTTCAAT CTCTAAGTAA TCATTTGTTT 
GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACGGA TGCAGTCTGG 
CTTGCCACGC AAAACCACAG CGCCCTGGTA ACCGAGACAA CGGTCGTCCC CTTTCTTCCG 
GTCAATCCTG AGTACTCATC AACACGAAAT CAGGGCAGAC AGAAGTTAGC TCGGTTCAAC 
GCCCATGAGT TTGCCACGCT GGTCATTGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAG 
GGCAGTTCTC TCTCGGGTTC AAAAGACAAT GTGGAGCTCA TACTGAAAAC CATCAATAAC 
CAGCACAGCG TTGAGAGTCA AGACAACGAT CAGCCCGACT ATGACAGCGT GGCATCAGAC 
GAAGACACAG ATTTGGAAAC CACTGCAAGC AAAACAAACC GGCAGAAGCT TCAAACACTC 
CAGAGTGAAA ATTCGAACCT CAGGAAACAG GCCACAACCA ATGTATATCA GGTGCAAACT 
GGTTCTGAGT ACACAGACAC TTCCAACCAC TCTTCCTTAA AGAGACGTCC GTCTGCCCGG 
GGCAGTAGGC CCATGTCCAT GTACGAGACC GGATCAGGTC AGAAACCATA TCTCCCAATG 
GGAGAAGCGA GCCGCCCCGA AGAGAGCAGG ATGAGACTCC AGCCCTTCCC CGCGCACGCA 
TCCAGGCTGG AGAAGCAGAA CAGCACACCT GAGAGTGACT ACGACAACAC TCCCAACGAC 
ATGGAGCCAG ATGGCATGGG GTCAAGCCGA AAGGGACGGC AAAGAAGTAT GGTGTGGCCA 
GGGGATGGCT TGGTACCAGA CACAGCAGAA CCCCATGTGG CCCCAAGCCC CACTCTCCCT 
AGCACCGAAG ATGTCATCAG GAAGACTGAA CAGATCACCA AAAACATACA GGAGCTCTTA 
AGAGCAGCCC AAGAAAATAA ACATGACAGT TATATTCCCT GCTCAGAGAG GATACACGTA 
GCTGTTACAG AAATGGCAGC ATTATTCCCC AAAAAACCCA AGTCTGATAT GGTGAGGACT 
TCCCTTCGTT TACTGACGTC CAGTGCCTAC CGACTGCAGT CAGAGTGCAA GAAGACCCTC 
CCAGGGGACC CCGGCTCACC CACAGACGTT CAGCTGGTCA CGCAGCAGGT CATCCAGTGT 
GCGTACGACA TCGCCAAGGC TGCCAAGCAG CTGGTTACCA TCACCACCAA AGAGAACAAC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( NM_001135214.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001128686.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu13920
Clone ID Related Accession (Same CDS sequence)	NM_001135214.3 , NM_001135214.2
Accession Version	NM_001135214.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP871813.1, AF124491.1, CR749340.1, D63482.2 and AA813937.1. On May 31, 2019 this sequence version replaced NM_001135214.2. Transcript Variant: This variant (5) lacks one in-frame exon in the 3' coding region, compared to variant 1. The resulting isoform (5) is missing one fragment but has the same N- and C-termini as isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.93400.1, SRR1660809.251574.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT 
ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA 
ACCAATGTAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC 
TTAAAGAGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACCGGATCA 
GGTCAGAAAC CATATCTCCC AATGGGAGAA GCGAGCCGCC CCGAAGAGAG CAGGATGAGA 
CTCCAGCCCT TCCCCGCGCA CGCATCCAGG CTGGAGAAGC AGAACAGCAC ACCTGAGAGT 
GACTACGACA ACACTCCCAA CGACATGGAG CCAGATGGCA TGGGGTCAAG CCGAAAGGGA 
CGGCAAAGAA GTATGGTGTG GCCAGGGGAT GGCTTGGTAC CAGACACAGC AGAACCCCAT 
GTGGCCCCAA GCCCCACTCT CCCTAGCACC GAAGATGTCA TCAGGAAGAC TGAACAGATC 
ACCAAAAACA TACAGGAGCT CTTAAGAGCA GCCCAAGAAA ATAAACATGA CAGTTATATT 
CCCTGCTCAG AGAGGATACA CGTAGCTGTT ACAGAAATGG CAGCATTATT CCCCAAAAAA 
CCCAAGTCTG ATATGGTGAG GACTTCCCTT CGTTTACTGA CGTCCAGTGC CTACCGACTG 
CAGTCAGAGT GCAAGAAGAC CCTCCCAGGG GACCCCGGCT CACCCACAGA CGTTCAGCTG 
GTCACGCAGC AGGTCATCCA GTGTGCGTAC GACATCGCCA AGGCTGCCAA GCAGCTGGTT 
ACCATCACCA CCAAAGAGAA CAACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128686.1
CDS	145..2334
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKVNNNLSDELRIMQKKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Target ORF information:

RefSeq Version	NM_001135214.3
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135214.3

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT 
ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA 
ACCAATGTAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC 
TTAAAGAGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACCGGATCA 
GGTCAGAAAC CATATCTCCC AATGGGAGAA GCGAGCCGCC CCGAAGAGAG CAGGATGAGA 
CTCCAGCCCT TCCCCGCGCA CGCATCCAGG CTGGAGAAGC AGAACAGCAC ACCTGAGAGT 
GACTACGACA ACACTCCCAA CGACATGGAG CCAGATGGCA TGGGGTCAAG CCGAAAGGGA 
CGGCAAAGAA GTATGGTGTG GCCAGGGGAT GGCTTGGTAC CAGACACAGC AGAACCCCAT 
GTGGCCCCAA GCCCCACTCT CCCTAGCACC GAAGATGTCA TCAGGAAGAC TGAACAGATC 
ACCAAAAACA TACAGGAGCT CTTAAGAGCA GCCCAAGAAA ATAAACATGA CAGTTATATT 
CCCTGCTCAG AGAGGATACA CGTAGCTGTT ACAGAAATGG CAGCATTATT CCCCAAAAAA 
CCCAAGTCTG ATATGGTGAG GACTTCCCTT CGTTTACTGA CGTCCAGTGC CTACCGACTG 
CAGTCAGAGT GCAAGAAGAC CCTCCCAGGG GACCCCGGCT CACCCACAGA CGTTCAGCTG 
GTCACGCAGC AGGTCATCCA GTGTGCGTAC GACATCGCCA AGGCTGCCAA GCAGCTGGTT 
ACCATCACCA CCAAAGAGAA CAACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( NM_001135214.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001128686.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 5, mRNA.

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CloneID	OHu13920
Clone ID Related Accession (Same CDS sequence)	NM_001135214.3 , NM_001135214.2
Accession Version	NM_001135214.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP871813.1, AF124491.1, CR749340.1, D63482.2 and AA813937.1. On Aug 18, 2016 this sequence version replaced NM_001135214.1. Transcript Variant: This variant (5) lacks one in-frame exon in the 3' coding region, compared to variant 1. The resulting isoform (5) is missing one fragment but has the same N- and C-termini as isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.93400.1, SRR1660809.251574.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT 
ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA 
ACCAATGTAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC 
TTAAAGAGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACCGGATCA 
GGTCAGAAAC CATATCTCCC AATGGGAGAA GCGAGCCGCC CCGAAGAGAG CAGGATGAGA 
CTCCAGCCCT TCCCCGCGCA CGCATCCAGG CTGGAGAAGC AGAACAGCAC ACCTGAGAGT 
GACTACGACA ACACTCCCAA CGACATGGAG CCAGATGGCA TGGGGTCAAG CCGAAAGGGA 
CGGCAAAGAA GTATGGTGTG GCCAGGGGAT GGCTTGGTAC CAGACACAGC AGAACCCCAT 
GTGGCCCCAA GCCCCACTCT CCCTAGCACC GAAGATGTCA TCAGGAAGAC TGAACAGATC 
ACCAAAAACA TACAGGAGCT CTTAAGAGCA GCCCAAGAAA ATAAACATGA CAGTTATATT 
CCCTGCTCAG AGAGGATACA CGTAGCTGTT ACAGAAATGG CAGCATTATT CCCCAAAAAA 
CCCAAGTCTG ATATGGTGAG GACTTCCCTT CGTTTACTGA CGTCCAGTGC CTACCGACTG 
CAGTCAGAGT GCAAGAAGAC CCTCCCAGGG GACCCCGGCT CACCCACAGA CGTTCAGCTG 
GTCACGCAGC AGGTCATCCA GTGTGCGTAC GACATCGCCA AGGCTGCCAA GCAGCTGGTT 
ACCATCACCA CCAAAGAGAA CAACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128686.1
CDS	166..2355
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKVNNNLSDELRIMQKKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Target ORF information:

RefSeq Version	NM_001135214.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135214.2

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT 
ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA 
ACCAATGTAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC 
TTAAAGAGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACCGGATCA 
GGTCAGAAAC CATATCTCCC AATGGGAGAA GCGAGCCGCC CCGAAGAGAG CAGGATGAGA 
CTCCAGCCCT TCCCCGCGCA CGCATCCAGG CTGGAGAAGC AGAACAGCAC ACCTGAGAGT 
GACTACGACA ACACTCCCAA CGACATGGAG CCAGATGGCA TGGGGTCAAG CCGAAAGGGA 
CGGCAAAGAA GTATGGTGTG GCCAGGGGAT GGCTTGGTAC CAGACACAGC AGAACCCCAT 
GTGGCCCCAA GCCCCACTCT CCCTAGCACC GAAGATGTCA TCAGGAAGAC TGAACAGATC 
ACCAAAAACA TACAGGAGCT CTTAAGAGCA GCCCAAGAAA ATAAACATGA CAGTTATATT 
CCCTGCTCAG AGAGGATACA CGTAGCTGTT ACAGAAATGG CAGCATTATT CCCCAAAAAA 
CCCAAGTCTG ATATGGTGAG GACTTCCCTT CGTTTACTGA CGTCCAGTGC CTACCGACTG 
CAGTCAGAGT GCAAGAAGAC CCTCCCAGGG GACCCCGGCT CACCCACAGA CGTTCAGCTG 
GTCACGCAGC AGGTCATCCA GTGTGCGTAC GACATCGCCA AGGCTGCCAA GCAGCTGGTT 
ACCATCACCA CCAAAGAGAA CAACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( NM_014776.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055591.2 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 3, mRNA.

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CloneID	OHu14013
Clone ID Related Accession (Same CDS sequence)	NM_014776.4 , NM_014776.5
Accession Version	NM_014776.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP871813.1, AF124491.1, D63482.2 and AA813937.1. On Aug 18, 2016 this sequence version replaced NM_014776.3. Transcript Variant: This variant (3) lacks two exons in the coding region, as compared to variant 1. Isoform 3 is missing two fragments but has the same N- and C-termini as isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGCTTCAAAC ACTCCAGAGT 
GAAAATTCGA ACCTCAGGAA ACAGGCCACA ACCAATGTAT ATCAGGTGCA AACTGGTTCT 
GAGTACACAG ACACTTCCAA CCACTCTTCC TTAAAGAGAC GTCCGTCTGC CCGGGGCAGT 
AGGCCCATGT CCATGTACGA GACCGGATCA GGTCAGAAAC CATATCTCCC AATGGGAGAA 
GCGAGCCGCC CCGAAGAGAG CAGGATGAGA CTCCAGCCCT TCCCCGCGCA CGCATCCAGG 
CTGGAGAAGC AGAACAGCAC ACCTGAGAGT GACTACGACA ACACTCCCAA CGACATGGAG 
CCAGATGGCA TGGGGTCAAG CCGAAAGGGA CGGCAAAGAA GTATGGTGTG GCCAGGGGAT 
GGCTTGGTAC CAGACACAGC AGAACCCCAT GTGGCCCCAA GCCCCACTCT CCCTAGCACC 
GAAGATGTCA TCAGGAAGAC TGAACAGATC ACCAAAAACA TACAGGAGCT CTTAAGAGCA 
GCCCAAGAAA ATAAACATGA CAGTTATATT CCCTGCTCAG AGAGGATACA CGTAGCTGTT 
ACAGAAATGG CAGCATTATT CCCCAAAAAA CCCAAGTCTG ATATGGTGAG GACTTCCCTT 
CGTTTACTGA CGTCCAGTGC CTACCGACTG CAGTCAGAGT GCAAGAAGAC CCTCCCAGGG 
GACCCCGGCT CACCCACAGA CGTTCAGCTG GTCACGCAGC AGGTCATCCA GTGTGCGTAC 
GACATCGCCA AGGCTGCCAA GCAGCTGGTT ACCATCACCA CCAAAGAGAA CAACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055591.2
CDS	166..2205
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Target ORF information:

RefSeq Version	NM_014776.4
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014776.4

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGCTTCAAAC ACTCCAGAGT 
GAAAATTCGA ACCTCAGGAA ACAGGCCACA ACCAATGTAT ATCAGGTGCA AACTGGTTCT 
GAGTACACAG ACACTTCCAA CCACTCTTCC TTAAAGAGAC GTCCGTCTGC CCGGGGCAGT 
AGGCCCATGT CCATGTACGA GACCGGATCA GGTCAGAAAC CATATCTCCC AATGGGAGAA 
GCGAGCCGCC CCGAAGAGAG CAGGATGAGA CTCCAGCCCT TCCCCGCGCA CGCATCCAGG 
CTGGAGAAGC AGAACAGCAC ACCTGAGAGT GACTACGACA ACACTCCCAA CGACATGGAG 
CCAGATGGCA TGGGGTCAAG CCGAAAGGGA CGGCAAAGAA GTATGGTGTG GCCAGGGGAT 
GGCTTGGTAC CAGACACAGC AGAACCCCAT GTGGCCCCAA GCCCCACTCT CCCTAGCACC 
GAAGATGTCA TCAGGAAGAC TGAACAGATC ACCAAAAACA TACAGGAGCT CTTAAGAGCA 
GCCCAAGAAA ATAAACATGA CAGTTATATT CCCTGCTCAG AGAGGATACA CGTAGCTGTT 
ACAGAAATGG CAGCATTATT CCCCAAAAAA CCCAAGTCTG ATATGGTGAG GACTTCCCTT 
CGTTTACTGA CGTCCAGTGC CTACCGACTG CAGTCAGAGT GCAAGAAGAC CCTCCCAGGG 
GACCCCGGCT CACCCACAGA CGTTCAGCTG GTCACGCAGC AGGTCATCCA GTGTGCGTAC 
GACATCGCCA AGGCTGCCAA GCAGCTGGTT ACCATCACCA CCAAAGAGAA CAACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( NM_014776.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_055591.2 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 3, mRNA.

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CloneID	OHu14013
Clone ID Related Accession (Same CDS sequence)	NM_014776.4 , NM_014776.5
Accession Version	NM_014776.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP871813.1, AF124491.1, D63482.2 and AA813937.1. On May 31, 2019 this sequence version replaced NM_014776.4. Transcript Variant: This variant (3) lacks two exons in the coding region, as compared to variant 1. Isoform 3 is missing two fragments but has the same N- and C-termini as isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGCTTCAAAC ACTCCAGAGT 
GAAAATTCGA ACCTCAGGAA ACAGGCCACA ACCAATGTAT ATCAGGTGCA AACTGGTTCT 
GAGTACACAG ACACTTCCAA CCACTCTTCC TTAAAGAGAC GTCCGTCTGC CCGGGGCAGT 
AGGCCCATGT CCATGTACGA GACCGGATCA GGTCAGAAAC CATATCTCCC AATGGGAGAA 
GCGAGCCGCC CCGAAGAGAG CAGGATGAGA CTCCAGCCCT TCCCCGCGCA CGCATCCAGG 
CTGGAGAAGC AGAACAGCAC ACCTGAGAGT GACTACGACA ACACTCCCAA CGACATGGAG 
CCAGATGGCA TGGGGTCAAG CCGAAAGGGA CGGCAAAGAA GTATGGTGTG GCCAGGGGAT 
GGCTTGGTAC CAGACACAGC AGAACCCCAT GTGGCCCCAA GCCCCACTCT CCCTAGCACC 
GAAGATGTCA TCAGGAAGAC TGAACAGATC ACCAAAAACA TACAGGAGCT CTTAAGAGCA 
GCCCAAGAAA ATAAACATGA CAGTTATATT CCCTGCTCAG AGAGGATACA CGTAGCTGTT 
ACAGAAATGG CAGCATTATT CCCCAAAAAA CCCAAGTCTG ATATGGTGAG GACTTCCCTT 
CGTTTACTGA CGTCCAGTGC CTACCGACTG CAGTCAGAGT GCAAGAAGAC CCTCCCAGGG 
GACCCCGGCT CACCCACAGA CGTTCAGCTG GTCACGCAGC AGGTCATCCA GTGTGCGTAC 
GACATCGCCA AGGCTGCCAA GCAGCTGGTT ACCATCACCA CCAAAGAGAA CAACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055591.2
CDS	145..2184
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Target ORF information:

RefSeq Version	NM_014776.5
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014776.5

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGCTTCAAAC ACTCCAGAGT 
GAAAATTCGA ACCTCAGGAA ACAGGCCACA ACCAATGTAT ATCAGGTGCA AACTGGTTCT 
GAGTACACAG ACACTTCCAA CCACTCTTCC TTAAAGAGAC GTCCGTCTGC CCGGGGCAGT 
AGGCCCATGT CCATGTACGA GACCGGATCA GGTCAGAAAC CATATCTCCC AATGGGAGAA 
GCGAGCCGCC CCGAAGAGAG CAGGATGAGA CTCCAGCCCT TCCCCGCGCA CGCATCCAGG 
CTGGAGAAGC AGAACAGCAC ACCTGAGAGT GACTACGACA ACACTCCCAA CGACATGGAG 
CCAGATGGCA TGGGGTCAAG CCGAAAGGGA CGGCAAAGAA GTATGGTGTG GCCAGGGGAT 
GGCTTGGTAC CAGACACAGC AGAACCCCAT GTGGCCCCAA GCCCCACTCT CCCTAGCACC 
GAAGATGTCA TCAGGAAGAC TGAACAGATC ACCAAAAACA TACAGGAGCT CTTAAGAGCA 
GCCCAAGAAA ATAAACATGA CAGTTATATT CCCTGCTCAG AGAGGATACA CGTAGCTGTT 
ACAGAAATGG CAGCATTATT CCCCAAAAAA CCCAAGTCTG ATATGGTGAG GACTTCCCTT 
CGTTTACTGA CGTCCAGTGC CTACCGACTG CAGTCAGAGT GCAAGAAGAC CCTCCCAGGG 
GACCCCGGCT CACCCACAGA CGTTCAGCTG GTCACGCAGC AGGTCATCCA GTGTGCGTAC 
GACATCGCCA AGGCTGCCAA GCAGCTGGTT ACCATCACCA CCAAAGAGAA CAACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( NM_001330153.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317082.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 7, mRNA.

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CloneID	OHu46998
Clone ID Related Accession (Same CDS sequence)	NM_001330153.1 , NM_001330153.2
Accession Version	NM_001330153.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP871813.1, AF124491.1, BE646544.1, CR980157.1, D63482.2 and AA813937.1. On Aug 18, 2016 this sequence version replaced XM_005254000.3. Transcript Variant: This variant (7) uses an alternate in-frame splice site and lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The resulting isoform (7) is shorter but has the same N- and C-termini as isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.154005.1, SRR1803617.88616.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCCTGGA TTTGTCTGAA 
TTGGCAAAAG CTGCTAAGAA GAAACTTCAA TCTCTAAGTA ATCATTTGTT TGAAGAACTT 
GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACGG ATGCAGTCTG GCTTGCCACG 
CAAAACCACA GCGCCCTGGT AACCGAGACA ACGGTCGTCC CCTTTCTTCC GGTCAATCCT 
GAGTACTCAT CAACACGAAA TCAGGGCAGA CAGAAGTTAG CTCGGTTCAA CGCCCATGAG 
TTTGCCACGC TGGTCATTGA CATTCTCAGT GACGCCAAGA GGAGACAGCA GGGCAGTTCT 
CTCTCGGGTT CAAAAGACAA TGTGGAGCTC ATACTGAAAA CCATCAATAA CCAGCACAGC 
GTTGAGAGTC AAGACAACGA TCAGCCCGAC TATGACAGCG TGGCATCAGA CGAAGACACA 
GATTTGGAAA CCACTGCAAG CAAAACAAAC CGGCAGAAGC TTCAAACACT CCAGAGTGAA 
AATTCGAACC TCAGGAAACA GGCCACAACC AATGTATATC AGGTGCAAAC TGGTTCTGAG 
TACACAGACA CTTCCAACCA CTCTTCCTTA AAGAGACGTC CGTCTGCCCG GGGCAGTAGG 
CCCATGTCCA TGTACGAGAC CGGATCAGGT CAGAAACCAT ATCTCCCAAT GGGAGAAGCG 
AGCCGCCCCG AAGAGAGCAG GATGAGACTC CAGCCCTTCC CCGCGCACAT CGGGAGGAGT 
GCACTTGTGA CCTCCTCTTC ATCTCTGCCT TCCTTCCCCT CCACACTTTC CTGGTCGAGG 
GACGAAAGCG CCCGAAGGGC ATCCAGGCTG GAGAAGCAGA ACAGCACACC TGAGAGTGAC 
TACGACAACA CTCCCAACGA CATGGAGCCA GATGGCATGG GGTCAAGCCG AAAGGGACGG 
CAAAGAAGTA TGGTGTGGCC AGGGGATGGC TTGGTACCAG ACACAGCAGA ACCCCATGTG 
GCCCCAAGCC CCACTCTCCC TAGCACCGAA GATGTCATCA GGAAGACTGA ACAGATCACC 
AAAAACATAC AGGAGCTCTT AAGAGCAGCC CAAGAAAATA AACATGACAG TTATATTCCC 
TGCTCAGAGA GGATACACGT AGCTGTTACA GAAATGGCAG CATTATTCCC CAAAAAACCC 
AAGTCTGATA TGGTGAGGAC TTCCCTTCGT TTACTGACGT CCAGTGCCTA CCGACTGCAG 
TCAGAGTGCA AGAAGACCCT CCCAGGGGAC CCCGGCTCAC CCACAGACGT TCAGCTGGTC 
ACGCAGCAGG TCATCCAGTG TGCGTACGAC ATCGCCAAGG CTGCCAAGCA GCTGGTTACC 
ATCACCACCA AAGAGAACAA CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317082.1
CDS	166..2292
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHIGRSALVTSSSSLPSFPSTLSWSRDESARRASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Target ORF information:

RefSeq Version	NM_001330153.1
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330153.1

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCCTGGA TTTGTCTGAA 
TTGGCAAAAG CTGCTAAGAA GAAACTTCAA TCTCTAAGTA ATCATTTGTT TGAAGAACTT 
GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACGG ATGCAGTCTG GCTTGCCACG 
CAAAACCACA GCGCCCTGGT AACCGAGACA ACGGTCGTCC CCTTTCTTCC GGTCAATCCT 
GAGTACTCAT CAACACGAAA TCAGGGCAGA CAGAAGTTAG CTCGGTTCAA CGCCCATGAG 
TTTGCCACGC TGGTCATTGA CATTCTCAGT GACGCCAAGA GGAGACAGCA GGGCAGTTCT 
CTCTCGGGTT CAAAAGACAA TGTGGAGCTC ATACTGAAAA CCATCAATAA CCAGCACAGC 
GTTGAGAGTC AAGACAACGA TCAGCCCGAC TATGACAGCG TGGCATCAGA CGAAGACACA 
GATTTGGAAA CCACTGCAAG CAAAACAAAC CGGCAGAAGC TTCAAACACT CCAGAGTGAA 
AATTCGAACC TCAGGAAACA GGCCACAACC AATGTATATC AGGTGCAAAC TGGTTCTGAG 
TACACAGACA CTTCCAACCA CTCTTCCTTA AAGAGACGTC CGTCTGCCCG GGGCAGTAGG 
CCCATGTCCA TGTACGAGAC CGGATCAGGT CAGAAACCAT ATCTCCCAAT GGGAGAAGCG 
AGCCGCCCCG AAGAGAGCAG GATGAGACTC CAGCCCTTCC CCGCGCACAT CGGGAGGAGT 
GCACTTGTGA CCTCCTCTTC ATCTCTGCCT TCCTTCCCCT CCACACTTTC CTGGTCGAGG 
GACGAAAGCG CCCGAAGGGC ATCCAGGCTG GAGAAGCAGA ACAGCACACC TGAGAGTGAC 
TACGACAACA CTCCCAACGA CATGGAGCCA GATGGCATGG GGTCAAGCCG AAAGGGACGG 
CAAAGAAGTA TGGTGTGGCC AGGGGATGGC TTGGTACCAG ACACAGCAGA ACCCCATGTG 
GCCCCAAGCC CCACTCTCCC TAGCACCGAA GATGTCATCA GGAAGACTGA ACAGATCACC 
AAAAACATAC AGGAGCTCTT AAGAGCAGCC CAAGAAAATA AACATGACAG TTATATTCCC 
TGCTCAGAGA GGATACACGT AGCTGTTACA GAAATGGCAG CATTATTCCC CAAAAAACCC 
AAGTCTGATA TGGTGAGGAC TTCCCTTCGT TTACTGACGT CCAGTGCCTA CCGACTGCAG 
TCAGAGTGCA AGAAGACCCT CCCAGGGGAC CCCGGCTCAC CCACAGACGT TCAGCTGGTC 
ACGCAGCAGG TCATCCAGTG TGCGTACGAC ATCGCCAAGG CTGCCAAGCA GCTGGTTACC 
ATCACCACCA AAGAGAACAA CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( NM_001330153.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317082.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 7, mRNA.

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CloneID	OHu46998
Clone ID Related Accession (Same CDS sequence)	NM_001330153.1 , NM_001330153.2
Accession Version	NM_001330153.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-31
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP871813.1, AF124491.1, BE646544.1, CR980157.1, D63482.2 and AA813937.1. On Jun 1, 2019 this sequence version replaced NM_001330153.1. Transcript Variant: This variant (7) uses an alternate in-frame splice site and lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The resulting isoform (7) is shorter but has the same N- and C-termini as isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.154005.1, SRR1803617.88616.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCCTGGA TTTGTCTGAA 
TTGGCAAAAG CTGCTAAGAA GAAACTTCAA TCTCTAAGTA ATCATTTGTT TGAAGAACTT 
GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACGG ATGCAGTCTG GCTTGCCACG 
CAAAACCACA GCGCCCTGGT AACCGAGACA ACGGTCGTCC CCTTTCTTCC GGTCAATCCT 
GAGTACTCAT CAACACGAAA TCAGGGCAGA CAGAAGTTAG CTCGGTTCAA CGCCCATGAG 
TTTGCCACGC TGGTCATTGA CATTCTCAGT GACGCCAAGA GGAGACAGCA GGGCAGTTCT 
CTCTCGGGTT CAAAAGACAA TGTGGAGCTC ATACTGAAAA CCATCAATAA CCAGCACAGC 
GTTGAGAGTC AAGACAACGA TCAGCCCGAC TATGACAGCG TGGCATCAGA CGAAGACACA 
GATTTGGAAA CCACTGCAAG CAAAACAAAC CGGCAGAAGC TTCAAACACT CCAGAGTGAA 
AATTCGAACC TCAGGAAACA GGCCACAACC AATGTATATC AGGTGCAAAC TGGTTCTGAG 
TACACAGACA CTTCCAACCA CTCTTCCTTA AAGAGACGTC CGTCTGCCCG GGGCAGTAGG 
CCCATGTCCA TGTACGAGAC CGGATCAGGT CAGAAACCAT ATCTCCCAAT GGGAGAAGCG 
AGCCGCCCCG AAGAGAGCAG GATGAGACTC CAGCCCTTCC CCGCGCACAT CGGGAGGAGT 
GCACTTGTGA CCTCCTCTTC ATCTCTGCCT TCCTTCCCCT CCACACTTTC CTGGTCGAGG 
GACGAAAGCG CCCGAAGGGC ATCCAGGCTG GAGAAGCAGA ACAGCACACC TGAGAGTGAC 
TACGACAACA CTCCCAACGA CATGGAGCCA GATGGCATGG GGTCAAGCCG AAAGGGACGG 
CAAAGAAGTA TGGTGTGGCC AGGGGATGGC TTGGTACCAG ACACAGCAGA ACCCCATGTG 
GCCCCAAGCC CCACTCTCCC TAGCACCGAA GATGTCATCA GGAAGACTGA ACAGATCACC 
AAAAACATAC AGGAGCTCTT AAGAGCAGCC CAAGAAAATA AACATGACAG TTATATTCCC 
TGCTCAGAGA GGATACACGT AGCTGTTACA GAAATGGCAG CATTATTCCC CAAAAAACCC 
AAGTCTGATA TGGTGAGGAC TTCCCTTCGT TTACTGACGT CCAGTGCCTA CCGACTGCAG 
TCAGAGTGCA AGAAGACCCT CCCAGGGGAC CCCGGCTCAC CCACAGACGT TCAGCTGGTC 
ACGCAGCAGG TCATCCAGTG TGCGTACGAC ATCGCCAAGG CTGCCAAGCA GCTGGTTACC 
ATCACCACCA AAGAGAACAA CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317082.1
CDS	145..2271
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHIGRSALVTSSSSLPSFPSTLSWSRDESARRASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Target ORF information:

RefSeq Version	NM_001330153.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330153.2

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCCTGGA TTTGTCTGAA 
TTGGCAAAAG CTGCTAAGAA GAAACTTCAA TCTCTAAGTA ATCATTTGTT TGAAGAACTT 
GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACGG ATGCAGTCTG GCTTGCCACG 
CAAAACCACA GCGCCCTGGT AACCGAGACA ACGGTCGTCC CCTTTCTTCC GGTCAATCCT 
GAGTACTCAT CAACACGAAA TCAGGGCAGA CAGAAGTTAG CTCGGTTCAA CGCCCATGAG 
TTTGCCACGC TGGTCATTGA CATTCTCAGT GACGCCAAGA GGAGACAGCA GGGCAGTTCT 
CTCTCGGGTT CAAAAGACAA TGTGGAGCTC ATACTGAAAA CCATCAATAA CCAGCACAGC 
GTTGAGAGTC AAGACAACGA TCAGCCCGAC TATGACAGCG TGGCATCAGA CGAAGACACA 
GATTTGGAAA CCACTGCAAG CAAAACAAAC CGGCAGAAGC TTCAAACACT CCAGAGTGAA 
AATTCGAACC TCAGGAAACA GGCCACAACC AATGTATATC AGGTGCAAAC TGGTTCTGAG 
TACACAGACA CTTCCAACCA CTCTTCCTTA AAGAGACGTC CGTCTGCCCG GGGCAGTAGG 
CCCATGTCCA TGTACGAGAC CGGATCAGGT CAGAAACCAT ATCTCCCAAT GGGAGAAGCG 
AGCCGCCCCG AAGAGAGCAG GATGAGACTC CAGCCCTTCC CCGCGCACAT CGGGAGGAGT 
GCACTTGTGA CCTCCTCTTC ATCTCTGCCT TCCTTCCCCT CCACACTTTC CTGGTCGAGG 
GACGAAAGCG CCCGAAGGGC ATCCAGGCTG GAGAAGCAGA ACAGCACACC TGAGAGTGAC 
TACGACAACA CTCCCAACGA CATGGAGCCA GATGGCATGG GGTCAAGCCG AAAGGGACGG 
CAAAGAAGTA TGGTGTGGCC AGGGGATGGC TTGGTACCAG ACACAGCAGA ACCCCATGTG 
GCCCCAAGCC CCACTCTCCC TAGCACCGAA GATGTCATCA GGAAGACTGA ACAGATCACC 
AAAAACATAC AGGAGCTCTT AAGAGCAGCC CAAGAAAATA AACATGACAG TTATATTCCC 
TGCTCAGAGA GGATACACGT AGCTGTTACA GAAATGGCAG CATTATTCCC CAAAAAACCC 
AAGTCTGATA TGGTGAGGAC TTCCCTTCGT TTACTGACGT CCAGTGCCTA CCGACTGCAG 
TCAGAGTGCA AGAAGACCCT CCCAGGGGAC CCCGGCTCAC CCACAGACGT TCAGCTGGTC 
ACGCAGCAGG TCATCCAGTG TGCGTACGAC ATCGCCAAGG CTGCCAAGCA GCTGGTTACC 
ATCACCACCA AAGAGAACAA CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( XM_005253997.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005254054.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X1, mRNA.

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CloneID	OHu46994
Clone ID Related Accession (Same CDS sequence)	XM_005253997.4 , XM_005253997.3
Accession Version	XM_005253997.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005253997.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCCTGGA TTTGTCTGAA 
TTGGCAAAAG CTGCTAAGAA GAAACTTCAA TCTCTAAGTA ATCATTTGTT TGAAGAACTT 
GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACGG ATGCAGTCTG GCTTGCCACG 
CAAAACCACA GCGCCCTGGT AACCGAGACA ACGGTCGTCC CCTTTCTTCC GGTCAATCCT 
GAGTACTCAT CAACACGAAA TCAGGGCAGA CAGAAGTTAG CTCGGTTCAA CGCCCATGAG 
TTTGCCACGC TGGTCATTGA CATTCTCAGT GACGCCAAGA GGAGACAGCA GGGCAGTTCT 
CTCTCGGGTT CAAAAGACAA TGTGGAGCTC ATACTGAAAA CCATCAATAA CCAGCACAGC 
GTTGAGAGTC AAGACAACGA TCAGCCCGAC TATGACAGCG TGGCATCAGA CGAAGACACA 
GATTTGGAAA CCACTGCAAG CAAAACAAAC CGGCAGAAGA GCCTAGATTC AGATTTATCA 
GATGGACCAG TCACTGTACA GGAATTTATG GAGGTCAAAA ACGCTCTAGT GGCTTCTGAG 
GCCAAGATAC AGCAGCTAAT GAAGGTGAAT AACAACTTGA GTGACGAGCT GAGAATTATG 
CAGAAAAAGC TTCAAACACT CCAGAGTGAA AATTCGAACC TCAGGAAACA GGCCACAACC 
AATGTATATC AGGTGCAAAC TGGTTCTGAG TACACAGACA CTTCCAACCA CTCTTCCTTA 
AAGAGACGTC CGTCTGCCCG GGGCAGTAGG CCCATGTCCA TGTACGAGAC CGGATCAGGT 
CAGAAACCAT ATCTCCCAAT GGGAGAAGCG AGCCGCCCCG AAGAGAGCAG GATGAGACTC 
CAGCCCTTCC CCGCGCACAT CGGGAGGAGT GCACTTGTGA CCTCCTCTTC ATCTCTGCCT 
TCCTTCCCCT CCACACTTTC CTGGTCGAGG GACGAAAGCG CCCGAAGGGC ATCCAGGCTG 
GAGAAGCAGA ACAGCACACC TGAGAGTGAC TACGACAACA CTCCCAACGA CATGGAGCCA 
GATGGCATGG GGTCAAGCCG AAAGGGACGG CAAAGAAGTA TGGTGTGGCC AGGGGATGGC 
TTGGTACCAG ACACAGCAGA ACCCCATGTG GCCCCAAGCC CCACTCTCCC TAGCACCGAA 
GATGTCATCA GGAAGACTGA ACAGATCACC AAAAACATAC AGGAGCTCTT AAGAGCAGCC 
CAAGAAAATA AACATGACAG TTATATTCCC TGCTCAGAGA GGATACACGT AGCTGTTACA 
GAAATGGCAG CATTATTCCC CAAAAAACCC AAGTCTGATA TGGTGAGGAC TTCCCTTCGT 
TTACTGACGT CCAGTGCCTA CCGACTGCAG TCAGAGTGCA AGAAGACCCT CCCAGGGGAC 
CCCGGCTCAC CCACAGACGT TCAGCTGGTC ACGCAGCAGG TCATCCAGTG TGCGTACGAC 
ATCGCCAAGG CTGCCAAGCA GCTGGTTACC ATCACCACCA AAGAGAACAA CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005254054.1
CDS	71..2347
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKVNNNLSDELRIMQKKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHIGRSALVTSSSSLPSFPSTLSWSRDESARRASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Target ORF information:

RefSeq Version	XM_005253997.4
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253997.4

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCCTGGA TTTGTCTGAA 
TTGGCAAAAG CTGCTAAGAA GAAACTTCAA TCTCTAAGTA ATCATTTGTT TGAAGAACTT 
GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACGG ATGCAGTCTG GCTTGCCACG 
CAAAACCACA GCGCCCTGGT AACCGAGACA ACGGTCGTCC CCTTTCTTCC GGTCAATCCT 
GAGTACTCAT CAACACGAAA TCAGGGCAGA CAGAAGTTAG CTCGGTTCAA CGCCCATGAG 
TTTGCCACGC TGGTCATTGA CATTCTCAGT GACGCCAAGA GGAGACAGCA GGGCAGTTCT 
CTCTCGGGTT CAAAAGACAA TGTGGAGCTC ATACTGAAAA CCATCAATAA CCAGCACAGC 
GTTGAGAGTC AAGACAACGA TCAGCCCGAC TATGACAGCG TGGCATCAGA CGAAGACACA 
GATTTGGAAA CCACTGCAAG CAAAACAAAC CGGCAGAAGA GCCTAGATTC AGATTTATCA 
GATGGACCAG TCACTGTACA GGAATTTATG GAGGTCAAAA ACGCTCTAGT GGCTTCTGAG 
GCCAAGATAC AGCAGCTAAT GAAGGTGAAT AACAACTTGA GTGACGAGCT GAGAATTATG 
CAGAAAAAGC TTCAAACACT CCAGAGTGAA AATTCGAACC TCAGGAAACA GGCCACAACC 
AATGTATATC AGGTGCAAAC TGGTTCTGAG TACACAGACA CTTCCAACCA CTCTTCCTTA 
AAGAGACGTC CGTCTGCCCG GGGCAGTAGG CCCATGTCCA TGTACGAGAC CGGATCAGGT 
CAGAAACCAT ATCTCCCAAT GGGAGAAGCG AGCCGCCCCG AAGAGAGCAG GATGAGACTC 
CAGCCCTTCC CCGCGCACAT CGGGAGGAGT GCACTTGTGA CCTCCTCTTC ATCTCTGCCT 
TCCTTCCCCT CCACACTTTC CTGGTCGAGG GACGAAAGCG CCCGAAGGGC ATCCAGGCTG 
GAGAAGCAGA ACAGCACACC TGAGAGTGAC TACGACAACA CTCCCAACGA CATGGAGCCA 
GATGGCATGG GGTCAAGCCG AAAGGGACGG CAAAGAAGTA TGGTGTGGCC AGGGGATGGC 
TTGGTACCAG ACACAGCAGA ACCCCATGTG GCCCCAAGCC CCACTCTCCC TAGCACCGAA 
GATGTCATCA GGAAGACTGA ACAGATCACC AAAAACATAC AGGAGCTCTT AAGAGCAGCC 
CAAGAAAATA AACATGACAG TTATATTCCC TGCTCAGAGA GGATACACGT AGCTGTTACA 
GAAATGGCAG CATTATTCCC CAAAAAACCC AAGTCTGATA TGGTGAGGAC TTCCCTTCGT 
TTACTGACGT CCAGTGCCTA CCGACTGCAG TCAGAGTGCA AGAAGACCCT CCCAGGGGAC 
CCCGGCTCAC CCACAGACGT TCAGCTGGTC ACGCAGCAGG TCATCCAGTG TGCGTACGAC 
ATCGCCAAGG CTGCCAAGCA GCTGGTTACC ATCACCACCA AAGAGAACAA CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( XM_005253997.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005254054.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu46994
Clone ID Related Accession (Same CDS sequence)	XM_005253997.4 , XM_005253997.3
Accession Version	XM_005253997.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005253997.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCCTGGA TTTGTCTGAA 
TTGGCAAAAG CTGCTAAGAA GAAACTTCAA TCTCTAAGTA ATCATTTGTT TGAAGAACTT 
GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACGG ATGCAGTCTG GCTTGCCACG 
CAAAACCACA GCGCCCTGGT AACCGAGACA ACGGTCGTCC CCTTTCTTCC GGTCAATCCT 
GAGTACTCAT CAACACGAAA TCAGGGCAGA CAGAAGTTAG CTCGGTTCAA CGCCCATGAG 
TTTGCCACGC TGGTCATTGA CATTCTCAGT GACGCCAAGA GGAGACAGCA GGGCAGTTCT 
CTCTCGGGTT CAAAAGACAA TGTGGAGCTC ATACTGAAAA CCATCAATAA CCAGCACAGC 
GTTGAGAGTC AAGACAACGA TCAGCCCGAC TATGACAGCG TGGCATCAGA CGAAGACACA 
GATTTGGAAA CCACTGCAAG CAAAACAAAC CGGCAGAAGA GCCTAGATTC AGATTTATCA 
GATGGACCAG TCACTGTACA GGAATTTATG GAGGTCAAAA ACGCTCTAGT GGCTTCTGAG 
GCCAAGATAC AGCAGCTAAT GAAGGTGAAT AACAACTTGA GTGACGAGCT GAGAATTATG 
CAGAAAAAGC TTCAAACACT CCAGAGTGAA AATTCGAACC TCAGGAAACA GGCCACAACC 
AATGTATATC AGGTGCAAAC TGGTTCTGAG TACACAGACA CTTCCAACCA CTCTTCCTTA 
AAGAGACGTC CGTCTGCCCG GGGCAGTAGG CCCATGTCCA TGTACGAGAC CGGATCAGGT 
CAGAAACCAT ATCTCCCAAT GGGAGAAGCG AGCCGCCCCG AAGAGAGCAG GATGAGACTC 
CAGCCCTTCC CCGCGCACAT CGGGAGGAGT GCACTTGTGA CCTCCTCTTC ATCTCTGCCT 
TCCTTCCCCT CCACACTTTC CTGGTCGAGG GACGAAAGCG CCCGAAGGGC ATCCAGGCTG 
GAGAAGCAGA ACAGCACACC TGAGAGTGAC TACGACAACA CTCCCAACGA CATGGAGCCA 
GATGGCATGG GGTCAAGCCG AAAGGGACGG CAAAGAAGTA TGGTGTGGCC AGGGGATGGC 
TTGGTACCAG ACACAGCAGA ACCCCATGTG GCCCCAAGCC CCACTCTCCC TAGCACCGAA 
GATGTCATCA GGAAGACTGA ACAGATCACC AAAAACATAC AGGAGCTCTT AAGAGCAGCC 
CAAGAAAATA AACATGACAG TTATATTCCC TGCTCAGAGA GGATACACGT AGCTGTTACA 
GAAATGGCAG CATTATTCCC CAAAAAACCC AAGTCTGATA TGGTGAGGAC TTCCCTTCGT 
TTACTGACGT CCAGTGCCTA CCGACTGCAG TCAGAGTGCA AGAAGACCCT CCCAGGGGAC 
CCCGGCTCAC CCACAGACGT TCAGCTGGTC ACGCAGCAGG TCATCCAGTG TGCGTACGAC 
ATCGCCAAGG CTGCCAAGCA GCTGGTTACC ATCACCACCA AAGAGAACAA CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005254054.1
CDS	161..2437
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKVNNNLSDELRIMQKKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHIGRSALVTSSSSLPSFPSTLSWSRDESARRASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Target ORF information:

RefSeq Version	XM_005253997.3
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253997.3

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCCTGGA TTTGTCTGAA 
TTGGCAAAAG CTGCTAAGAA GAAACTTCAA TCTCTAAGTA ATCATTTGTT TGAAGAACTT 
GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACGG ATGCAGTCTG GCTTGCCACG 
CAAAACCACA GCGCCCTGGT AACCGAGACA ACGGTCGTCC CCTTTCTTCC GGTCAATCCT 
GAGTACTCAT CAACACGAAA TCAGGGCAGA CAGAAGTTAG CTCGGTTCAA CGCCCATGAG 
TTTGCCACGC TGGTCATTGA CATTCTCAGT GACGCCAAGA GGAGACAGCA GGGCAGTTCT 
CTCTCGGGTT CAAAAGACAA TGTGGAGCTC ATACTGAAAA CCATCAATAA CCAGCACAGC 
GTTGAGAGTC AAGACAACGA TCAGCCCGAC TATGACAGCG TGGCATCAGA CGAAGACACA 
GATTTGGAAA CCACTGCAAG CAAAACAAAC CGGCAGAAGA GCCTAGATTC AGATTTATCA 
GATGGACCAG TCACTGTACA GGAATTTATG GAGGTCAAAA ACGCTCTAGT GGCTTCTGAG 
GCCAAGATAC AGCAGCTAAT GAAGGTGAAT AACAACTTGA GTGACGAGCT GAGAATTATG 
CAGAAAAAGC TTCAAACACT CCAGAGTGAA AATTCGAACC TCAGGAAACA GGCCACAACC 
AATGTATATC AGGTGCAAAC TGGTTCTGAG TACACAGACA CTTCCAACCA CTCTTCCTTA 
AAGAGACGTC CGTCTGCCCG GGGCAGTAGG CCCATGTCCA TGTACGAGAC CGGATCAGGT 
CAGAAACCAT ATCTCCCAAT GGGAGAAGCG AGCCGCCCCG AAGAGAGCAG GATGAGACTC 
CAGCCCTTCC CCGCGCACAT CGGGAGGAGT GCACTTGTGA CCTCCTCTTC ATCTCTGCCT 
TCCTTCCCCT CCACACTTTC CTGGTCGAGG GACGAAAGCG CCCGAAGGGC ATCCAGGCTG 
GAGAAGCAGA ACAGCACACC TGAGAGTGAC TACGACAACA CTCCCAACGA CATGGAGCCA 
GATGGCATGG GGTCAAGCCG AAAGGGACGG CAAAGAAGTA TGGTGTGGCC AGGGGATGGC 
TTGGTACCAG ACACAGCAGA ACCCCATGTG GCCCCAAGCC CCACTCTCCC TAGCACCGAA 
GATGTCATCA GGAAGACTGA ACAGATCACC AAAAACATAC AGGAGCTCTT AAGAGCAGCC 
CAAGAAAATA AACATGACAG TTATATTCCC TGCTCAGAGA GGATACACGT AGCTGTTACA 
GAAATGGCAG CATTATTCCC CAAAAAACCC AAGTCTGATA TGGTGAGGAC TTCCCTTCGT 
TTACTGACGT CCAGTGCCTA CCGACTGCAG TCAGAGTGCA AGAAGACCCT CCCAGGGGAC 
CCCGGCTCAC CCACAGACGT TCAGCTGGTC ACGCAGCAGG TCATCCAGTG TGCGTACGAC 
ATCGCCAAGG CTGCCAAGCA GCTGGTTACC ATCACCACCA AAGAGAACAA CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( XM_006719707.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719770.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X2, mRNA.

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CloneID	OHu46995
Clone ID Related Accession (Same CDS sequence)	XM_006719707.4 , XM_006719707.3
Accession Version	XM_006719707.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006719707.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGCTT CAAACACTCC AGAGTGAAAA TTCGAACCTC 
AGGAAACAGG CCACAACCAA TGTATATCAG GTGCAAACTG GTTCTGAGTA CACAGACACT 
TCCAACCACT CTTCCTTAAA GAGACGTCCG TCTGCCCGGG GCAGTAGGCC CATGTCCATG 
TACGAGACCG GATCAGGTCA GAAACCATAT CTCCCAATGG GAGAAGCGAG CCGCCCCGAA 
GAGAGCAGGA TGAGACTCCA GCCCTTCCCC GCGCACATCG GGAGGAGTGC ACTTGTGACC 
TCCTCTTCAT CTCTGCCTTC CTTCCCCTCC ACACTTTCCT GGTCGAGGGA CGAAAGCGCC 
CGAAGGGCAT CCAGGCTGGA GAAGCAGAAC AGCACACCTG AGAGTGACTA CGACAACACT 
CCCAACGACA TGGAGCCAGA TGGCATGGGG TCAAGCCGAA AGGGACGGCA AAGAAGTATG 
GTGTGGCCAG GGGATGGCTT GGTACCAGAC ACAGCAGAAC CCCATGTGGC CCCAAGCCCC 
ACTCTCCCTA GCACCGAAGA TGTCATCAGG AAGACTGAAC AGATCACCAA AAACATACAG 
GAGCTCTTAA GAGCAGCCCA AGAAAATAAA CATGACAGTT ATATTCCCTG CTCAGAGAGG 
ATACACGTAG CTGTTACAGA AATGGCAGCA TTATTCCCCA AAAAACCCAA GTCTGATATG 
GTGAGGACTT CCCTTCGTTT ACTGACGTCC AGTGCCTACC GACTGCAGTC AGAGTGCAAG 
AAGACCCTCC CAGGGGACCC CGGCTCACCC ACAGACGTTC AGCTGGTCAC GCAGCAGGTC 
ATCCAGTGTG CGTACGACAT CGCCAAGGCT GCCAAGCAGC TGGTTACCAT CACCACCAAA 
GAGAACAACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719770.1
CDS	71..2305
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHIGRSALVTSSSSLPSFPSTLSWSRDESARRASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Target ORF information:

RefSeq Version	XM_006719707.4
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006719707.4

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGCTT CAAACACTCC AGAGTGAAAA TTCGAACCTC 
AGGAAACAGG CCACAACCAA TGTATATCAG GTGCAAACTG GTTCTGAGTA CACAGACACT 
TCCAACCACT CTTCCTTAAA GAGACGTCCG TCTGCCCGGG GCAGTAGGCC CATGTCCATG 
TACGAGACCG GATCAGGTCA GAAACCATAT CTCCCAATGG GAGAAGCGAG CCGCCCCGAA 
GAGAGCAGGA TGAGACTCCA GCCCTTCCCC GCGCACATCG GGAGGAGTGC ACTTGTGACC 
TCCTCTTCAT CTCTGCCTTC CTTCCCCTCC ACACTTTCCT GGTCGAGGGA CGAAAGCGCC 
CGAAGGGCAT CCAGGCTGGA GAAGCAGAAC AGCACACCTG AGAGTGACTA CGACAACACT 
CCCAACGACA TGGAGCCAGA TGGCATGGGG TCAAGCCGAA AGGGACGGCA AAGAAGTATG 
GTGTGGCCAG GGGATGGCTT GGTACCAGAC ACAGCAGAAC CCCATGTGGC CCCAAGCCCC 
ACTCTCCCTA GCACCGAAGA TGTCATCAGG AAGACTGAAC AGATCACCAA AAACATACAG 
GAGCTCTTAA GAGCAGCCCA AGAAAATAAA CATGACAGTT ATATTCCCTG CTCAGAGAGG 
ATACACGTAG CTGTTACAGA AATGGCAGCA TTATTCCCCA AAAAACCCAA GTCTGATATG 
GTGAGGACTT CCCTTCGTTT ACTGACGTCC AGTGCCTACC GACTGCAGTC AGAGTGCAAG 
AAGACCCTCC CAGGGGACCC CGGCTCACCC ACAGACGTTC AGCTGGTCAC GCAGCAGGTC 
ATCCAGTGTG CGTACGACAT CGCCAAGGCT GCCAAGCAGC TGGTTACCAT CACCACCAAA 
GAGAACAACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( XM_006719707.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719770.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu46995
Clone ID Related Accession (Same CDS sequence)	XM_006719707.4 , XM_006719707.3
Accession Version	XM_006719707.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006719707.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGCTT CAAACACTCC AGAGTGAAAA TTCGAACCTC 
AGGAAACAGG CCACAACCAA TGTATATCAG GTGCAAACTG GTTCTGAGTA CACAGACACT 
TCCAACCACT CTTCCTTAAA GAGACGTCCG TCTGCCCGGG GCAGTAGGCC CATGTCCATG 
TACGAGACCG GATCAGGTCA GAAACCATAT CTCCCAATGG GAGAAGCGAG CCGCCCCGAA 
GAGAGCAGGA TGAGACTCCA GCCCTTCCCC GCGCACATCG GGAGGAGTGC ACTTGTGACC 
TCCTCTTCAT CTCTGCCTTC CTTCCCCTCC ACACTTTCCT GGTCGAGGGA CGAAAGCGCC 
CGAAGGGCAT CCAGGCTGGA GAAGCAGAAC AGCACACCTG AGAGTGACTA CGACAACACT 
CCCAACGACA TGGAGCCAGA TGGCATGGGG TCAAGCCGAA AGGGACGGCA AAGAAGTATG 
GTGTGGCCAG GGGATGGCTT GGTACCAGAC ACAGCAGAAC CCCATGTGGC CCCAAGCCCC 
ACTCTCCCTA GCACCGAAGA TGTCATCAGG AAGACTGAAC AGATCACCAA AAACATACAG 
GAGCTCTTAA GAGCAGCCCA AGAAAATAAA CATGACAGTT ATATTCCCTG CTCAGAGAGG 
ATACACGTAG CTGTTACAGA AATGGCAGCA TTATTCCCCA AAAAACCCAA GTCTGATATG 
GTGAGGACTT CCCTTCGTTT ACTGACGTCC AGTGCCTACC GACTGCAGTC AGAGTGCAAG 
AAGACCCTCC CAGGGGACCC CGGCTCACCC ACAGACGTTC AGCTGGTCAC GCAGCAGGTC 
ATCCAGTGTG CGTACGACAT CGCCAAGGCT GCCAAGCAGC TGGTTACCAT CACCACCAAA 
GAGAACAACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719770.1
CDS	161..2395
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHIGRSALVTSSSSLPSFPSTLSWSRDESARRASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Target ORF information:

RefSeq Version	XM_006719707.3
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006719707.3

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGCTT CAAACACTCC AGAGTGAAAA TTCGAACCTC 
AGGAAACAGG CCACAACCAA TGTATATCAG GTGCAAACTG GTTCTGAGTA CACAGACACT 
TCCAACCACT CTTCCTTAAA GAGACGTCCG TCTGCCCGGG GCAGTAGGCC CATGTCCATG 
TACGAGACCG GATCAGGTCA GAAACCATAT CTCCCAATGG GAGAAGCGAG CCGCCCCGAA 
GAGAGCAGGA TGAGACTCCA GCCCTTCCCC GCGCACATCG GGAGGAGTGC ACTTGTGACC 
TCCTCTTCAT CTCTGCCTTC CTTCCCCTCC ACACTTTCCT GGTCGAGGGA CGAAAGCGCC 
CGAAGGGCAT CCAGGCTGGA GAAGCAGAAC AGCACACCTG AGAGTGACTA CGACAACACT 
CCCAACGACA TGGAGCCAGA TGGCATGGGG TCAAGCCGAA AGGGACGGCA AAGAAGTATG 
GTGTGGCCAG GGGATGGCTT GGTACCAGAC ACAGCAGAAC CCCATGTGGC CCCAAGCCCC 
ACTCTCCCTA GCACCGAAGA TGTCATCAGG AAGACTGAAC AGATCACCAA AAACATACAG 
GAGCTCTTAA GAGCAGCCCA AGAAAATAAA CATGACAGTT ATATTCCCTG CTCAGAGAGG 
ATACACGTAG CTGTTACAGA AATGGCAGCA TTATTCCCCA AAAAACCCAA GTCTGATATG 
GTGAGGACTT CCCTTCGTTT ACTGACGTCC AGTGCCTACC GACTGCAGTC AGAGTGCAAG 
AAGACCCTCC CAGGGGACCC CGGCTCACCC ACAGACGTTC AGCTGGTCAC GCAGCAGGTC 
ATCCAGTGTG CGTACGACAT CGCCAAGGCT GCCAAGCAGC TGGTTACCAT CACCACCAAA 
GAGAACAACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( XM_006719708.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719771.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu46996
Clone ID Related Accession (Same CDS sequence)	XM_006719708.3 , XM_006719708.4
Accession Version	XM_006719708.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006719708.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGCTT CAAACACTCC AGAGTGAAAA TTCGAACCTC 
AGGAAACAGG CCACAACCAA TGTATATCAG GTGCAAACTG GTTCTGAGTA CACAGACACT 
TCCAACCACT CTTCCTTAAA GAGACGTCCG TCTGCCCGGG GCAGTAGGCC CATGTCCATG 
TACGAGACCG GATCAGGTCA GAAACCATAT CTCCCAATGG GAGAAGCGAG CCGCCCCGAA 
GAGAGCAGGA TGAGACTCCA GCCCTTCCCC GCGCACGCAT CCAGGCTGGA GAAGCAGAAC 
AGCACACCTG AGAGTGACTA CGACAACACT CCCAACGACA TGGAGCCAGA TGGCATGGGG 
TCAAGCCGAA AGGGACGGCA AAGAAGTATG GTGTGGCCAG GGGATGGCTT GGTACCAGAC 
ACAGCAGAAC CCCATGTGGC CCCAAGCCCC ACTCTCCCTA GCACCGAAGA TGTCATCAGG 
AAGACTGAAC AGATCACCAA AAACATACAG GAGCTCTTAA GAGCAGCCCA AGAAAATAAA 
CATGACAGTT ATATTCCCTG CTCAGAGAGG ATACACGTAG CTGTTACAGA AATGGCAGCA 
TTATTCCCCA AAAAACCCAA GTCTGATATG GTGAGGACTT CCCTTCGTTT ACTGACGTCC 
AGTGCCTACC GACTGCAGTC AGAGTGCAAG AAGACCCTCC CAGGGGACCC CGGCTCACCC 
ACAGACGTTC AGCTGGTCAC GCAGCAGGTC ATCCAGTGTG CGTACGACAT CGCCAAGGCT 
GCCAAGCAGC TGGTTACCAT CACCACCAAA GAGAACAACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719771.1
CDS	161..2305
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Target ORF information:

RefSeq Version	XM_006719708.3
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006719708.3

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGCTT CAAACACTCC AGAGTGAAAA TTCGAACCTC 
AGGAAACAGG CCACAACCAA TGTATATCAG GTGCAAACTG GTTCTGAGTA CACAGACACT 
TCCAACCACT CTTCCTTAAA GAGACGTCCG TCTGCCCGGG GCAGTAGGCC CATGTCCATG 
TACGAGACCG GATCAGGTCA GAAACCATAT CTCCCAATGG GAGAAGCGAG CCGCCCCGAA 
GAGAGCAGGA TGAGACTCCA GCCCTTCCCC GCGCACGCAT CCAGGCTGGA GAAGCAGAAC 
AGCACACCTG AGAGTGACTA CGACAACACT CCCAACGACA TGGAGCCAGA TGGCATGGGG 
TCAAGCCGAA AGGGACGGCA AAGAAGTATG GTGTGGCCAG GGGATGGCTT GGTACCAGAC 
ACAGCAGAAC CCCATGTGGC CCCAAGCCCC ACTCTCCCTA GCACCGAAGA TGTCATCAGG 
AAGACTGAAC AGATCACCAA AAACATACAG GAGCTCTTAA GAGCAGCCCA AGAAAATAAA 
CATGACAGTT ATATTCCCTG CTCAGAGAGG ATACACGTAG CTGTTACAGA AATGGCAGCA 
TTATTCCCCA AAAAACCCAA GTCTGATATG GTGAGGACTT CCCTTCGTTT ACTGACGTCC 
AGTGCCTACC GACTGCAGTC AGAGTGCAAG AAGACCCTCC CAGGGGACCC CGGCTCACCC 
ACAGACGTTC AGCTGGTCAC GCAGCAGGTC ATCCAGTGTG CGTACGACAT CGCCAAGGCT 
GCCAAGCAGC TGGTTACCAT CACCACCAAA GAGAACAACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( XM_006719708.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719771.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu46996
Clone ID Related Accession (Same CDS sequence)	XM_006719708.3 , XM_006719708.4
Accession Version	XM_006719708.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006719708.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGCTT CAAACACTCC AGAGTGAAAA TTCGAACCTC 
AGGAAACAGG CCACAACCAA TGTATATCAG GTGCAAACTG GTTCTGAGTA CACAGACACT 
TCCAACCACT CTTCCTTAAA GAGACGTCCG TCTGCCCGGG GCAGTAGGCC CATGTCCATG 
TACGAGACCG GATCAGGTCA GAAACCATAT CTCCCAATGG GAGAAGCGAG CCGCCCCGAA 
GAGAGCAGGA TGAGACTCCA GCCCTTCCCC GCGCACGCAT CCAGGCTGGA GAAGCAGAAC 
AGCACACCTG AGAGTGACTA CGACAACACT CCCAACGACA TGGAGCCAGA TGGCATGGGG 
TCAAGCCGAA AGGGACGGCA AAGAAGTATG GTGTGGCCAG GGGATGGCTT GGTACCAGAC 
ACAGCAGAAC CCCATGTGGC CCCAAGCCCC ACTCTCCCTA GCACCGAAGA TGTCATCAGG 
AAGACTGAAC AGATCACCAA AAACATACAG GAGCTCTTAA GAGCAGCCCA AGAAAATAAA 
CATGACAGTT ATATTCCCTG CTCAGAGAGG ATACACGTAG CTGTTACAGA AATGGCAGCA 
TTATTCCCCA AAAAACCCAA GTCTGATATG GTGAGGACTT CCCTTCGTTT ACTGACGTCC 
AGTGCCTACC GACTGCAGTC AGAGTGCAAG AAGACCCTCC CAGGGGACCC CGGCTCACCC 
ACAGACGTTC AGCTGGTCAC GCAGCAGGTC ATCCAGTGTG CGTACGACAT CGCCAAGGCT 
GCCAAGCAGC TGGTTACCAT CACCACCAAA GAGAACAACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719771.1
CDS	71..2215
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Target ORF information:

RefSeq Version	XM_006719708.4
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006719708.4

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGCTT CAAACACTCC AGAGTGAAAA TTCGAACCTC 
AGGAAACAGG CCACAACCAA TGTATATCAG GTGCAAACTG GTTCTGAGTA CACAGACACT 
TCCAACCACT CTTCCTTAAA GAGACGTCCG TCTGCCCGGG GCAGTAGGCC CATGTCCATG 
TACGAGACCG GATCAGGTCA GAAACCATAT CTCCCAATGG GAGAAGCGAG CCGCCCCGAA 
GAGAGCAGGA TGAGACTCCA GCCCTTCCCC GCGCACGCAT CCAGGCTGGA GAAGCAGAAC 
AGCACACCTG AGAGTGACTA CGACAACACT CCCAACGACA TGGAGCCAGA TGGCATGGGG 
TCAAGCCGAA AGGGACGGCA AAGAAGTATG GTGTGGCCAG GGGATGGCTT GGTACCAGAC 
ACAGCAGAAC CCCATGTGGC CCCAAGCCCC ACTCTCCCTA GCACCGAAGA TGTCATCAGG 
AAGACTGAAC AGATCACCAA AAACATACAG GAGCTCTTAA GAGCAGCCCA AGAAAATAAA 
CATGACAGTT ATATTCCCTG CTCAGAGAGG ATACACGTAG CTGTTACAGA AATGGCAGCA 
TTATTCCCCA AAAAACCCAA GTCTGATATG GTGAGGACTT CCCTTCGTTT ACTGACGTCC 
AGTGCCTACC GACTGCAGTC AGAGTGCAAG AAGACCCTCC CAGGGGACCC CGGCTCACCC 
ACAGACGTTC AGCTGGTCAC GCAGCAGGTC ATCCAGTGTG CGTACGACAT CGCCAAGGCT 
GCCAAGCAGC TGGTTACCAT CACCACCAAA GAGAACAACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( XM_006719709.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719772.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu46997
Clone ID Related Accession (Same CDS sequence)	XM_006719709.4 , XM_006719709.3
Accession Version	XM_006719709.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006719709.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGCTTCAAAC ACTCCAGAGT 
GAAAATTCGA ACCTCAGGAA ACAGGCCACA ACCAATGTAT ATCAGGTGCA AACTGGTTCT 
GAGTACACAG ACACTTCCAA CCACTCTTCC TTAAAGAGAC GTCCGTCTGC CCGGGGCAGT 
AGGCCCATGT CCATGTACGA GACCGGATCA GGTCAGAAAC CATATCTCCC AATGGGAGAA 
GCGAGCCGCC CCGAAGAGAG CAGGATGAGA CTCCAGCCCT TCCCCGCGCA CATCGGGAGG 
AGTGCACTTG TGACCTCCTC TTCATCTCTG CCTTCCTTCC CCTCCACACT TTCCTGGTCG 
AGGGACGAAA GCGCCCGAAG GGCATCCAGG CTGGAGAAGC AGAACAGCAC ACCTGAGAGT 
GACTACGACA ACACTCCCAA CGACATGGAG CCAGATGGCA TGGGGTCAAG CCGAAAGGGA 
CGGCAAAGAA GTATGGTGTG GCCAGGGGAT GGCTTGGTAC CAGACACAGC AGAACCCCAT 
GTGGCCCCAA GCCCCACTCT CCCTAGCACC GAAGATGTCA TCAGGAAGAC TGAACAGATC 
ACCAAAAACA TACAGGAGCT CTTAAGAGCA GCCCAAGAAA ATAAACATGA CAGTTATATT 
CCCTGCTCAG AGAGGATACA CGTAGCTGTT ACAGAAATGG CAGCATTATT CCCCAAAAAA 
CCCAAGTCTG ATATGGTGAG GACTTCCCTT CGTTTACTGA CGTCCAGTGC CTACCGACTG 
CAGTCAGAGT GCAAGAAGAC CCTCCCAGGG GACCCCGGCT CACCCACAGA CGTTCAGCTG 
GTCACGCAGC AGGTCATCCA GTGTGCGTAC GACATCGCCA AGGCTGCCAA GCAGCTGGTT 
ACCATCACCA CCAAAGAGAA CAACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719772.1
CDS	71..2200
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHIGRSALVTSSSSLPSFPSTLSWSRDESARRASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Target ORF information:

RefSeq Version	XM_006719709.4
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006719709.4

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGCTTCAAAC ACTCCAGAGT 
GAAAATTCGA ACCTCAGGAA ACAGGCCACA ACCAATGTAT ATCAGGTGCA AACTGGTTCT 
GAGTACACAG ACACTTCCAA CCACTCTTCC TTAAAGAGAC GTCCGTCTGC CCGGGGCAGT 
AGGCCCATGT CCATGTACGA GACCGGATCA GGTCAGAAAC CATATCTCCC AATGGGAGAA 
GCGAGCCGCC CCGAAGAGAG CAGGATGAGA CTCCAGCCCT TCCCCGCGCA CATCGGGAGG 
AGTGCACTTG TGACCTCCTC TTCATCTCTG CCTTCCTTCC CCTCCACACT TTCCTGGTCG 
AGGGACGAAA GCGCCCGAAG GGCATCCAGG CTGGAGAAGC AGAACAGCAC ACCTGAGAGT 
GACTACGACA ACACTCCCAA CGACATGGAG CCAGATGGCA TGGGGTCAAG CCGAAAGGGA 
CGGCAAAGAA GTATGGTGTG GCCAGGGGAT GGCTTGGTAC CAGACACAGC AGAACCCCAT 
GTGGCCCCAA GCCCCACTCT CCCTAGCACC GAAGATGTCA TCAGGAAGAC TGAACAGATC 
ACCAAAAACA TACAGGAGCT CTTAAGAGCA GCCCAAGAAA ATAAACATGA CAGTTATATT 
CCCTGCTCAG AGAGGATACA CGTAGCTGTT ACAGAAATGG CAGCATTATT CCCCAAAAAA 
CCCAAGTCTG ATATGGTGAG GACTTCCCTT CGTTTACTGA CGTCCAGTGC CTACCGACTG 
CAGTCAGAGT GCAAGAAGAC CCTCCCAGGG GACCCCGGCT CACCCACAGA CGTTCAGCTG 
GTCACGCAGC AGGTCATCCA GTGTGCGTAC GACATCGCCA AGGCTGCCAA GCAGCTGGTT 
ACCATCACCA CCAAAGAGAA CAACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( XM_006719709.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719772.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X5, mRNA.

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CloneID	OHu46997
Clone ID Related Accession (Same CDS sequence)	XM_006719709.4 , XM_006719709.3
Accession Version	XM_006719709.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006719709.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGCTTCAAAC ACTCCAGAGT 
GAAAATTCGA ACCTCAGGAA ACAGGCCACA ACCAATGTAT ATCAGGTGCA AACTGGTTCT 
GAGTACACAG ACACTTCCAA CCACTCTTCC TTAAAGAGAC GTCCGTCTGC CCGGGGCAGT 
AGGCCCATGT CCATGTACGA GACCGGATCA GGTCAGAAAC CATATCTCCC AATGGGAGAA 
GCGAGCCGCC CCGAAGAGAG CAGGATGAGA CTCCAGCCCT TCCCCGCGCA CATCGGGAGG 
AGTGCACTTG TGACCTCCTC TTCATCTCTG CCTTCCTTCC CCTCCACACT TTCCTGGTCG 
AGGGACGAAA GCGCCCGAAG GGCATCCAGG CTGGAGAAGC AGAACAGCAC ACCTGAGAGT 
GACTACGACA ACACTCCCAA CGACATGGAG CCAGATGGCA TGGGGTCAAG CCGAAAGGGA 
CGGCAAAGAA GTATGGTGTG GCCAGGGGAT GGCTTGGTAC CAGACACAGC AGAACCCCAT 
GTGGCCCCAA GCCCCACTCT CCCTAGCACC GAAGATGTCA TCAGGAAGAC TGAACAGATC 
ACCAAAAACA TACAGGAGCT CTTAAGAGCA GCCCAAGAAA ATAAACATGA CAGTTATATT 
CCCTGCTCAG AGAGGATACA CGTAGCTGTT ACAGAAATGG CAGCATTATT CCCCAAAAAA 
CCCAAGTCTG ATATGGTGAG GACTTCCCTT CGTTTACTGA CGTCCAGTGC CTACCGACTG 
CAGTCAGAGT GCAAGAAGAC CCTCCCAGGG GACCCCGGCT CACCCACAGA CGTTCAGCTG 
GTCACGCAGC AGGTCATCCA GTGTGCGTAC GACATCGCCA AGGCTGCCAA GCAGCTGGTT 
ACCATCACCA CCAAAGAGAA CAACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719772.1
CDS	161..2290
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHIGRSALVTSSSSLPSFPSTLSWSRDESARRASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Target ORF information:

RefSeq Version	XM_006719709.3
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006719709.3

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGCTTCAAAC ACTCCAGAGT 
GAAAATTCGA ACCTCAGGAA ACAGGCCACA ACCAATGTAT ATCAGGTGCA AACTGGTTCT 
GAGTACACAG ACACTTCCAA CCACTCTTCC TTAAAGAGAC GTCCGTCTGC CCGGGGCAGT 
AGGCCCATGT CCATGTACGA GACCGGATCA GGTCAGAAAC CATATCTCCC AATGGGAGAA 
GCGAGCCGCC CCGAAGAGAG CAGGATGAGA CTCCAGCCCT TCCCCGCGCA CATCGGGAGG 
AGTGCACTTG TGACCTCCTC TTCATCTCTG CCTTCCTTCC CCTCCACACT TTCCTGGTCG 
AGGGACGAAA GCGCCCGAAG GGCATCCAGG CTGGAGAAGC AGAACAGCAC ACCTGAGAGT 
GACTACGACA ACACTCCCAA CGACATGGAG CCAGATGGCA TGGGGTCAAG CCGAAAGGGA 
CGGCAAAGAA GTATGGTGTG GCCAGGGGAT GGCTTGGTAC CAGACACAGC AGAACCCCAT 
GTGGCCCCAA GCCCCACTCT CCCTAGCACC GAAGATGTCA TCAGGAAGAC TGAACAGATC 
ACCAAAAACA TACAGGAGCT CTTAAGAGCA GCCCAAGAAA ATAAACATGA CAGTTATATT 
CCCTGCTCAG AGAGGATACA CGTAGCTGTT ACAGAAATGG CAGCATTATT CCCCAAAAAA 
CCCAAGTCTG ATATGGTGAG GACTTCCCTT CGTTTACTGA CGTCCAGTGC CTACCGACTG 
CAGTCAGAGT GCAAGAAGAC CCTCCCAGGG GACCCCGGCT CACCCACAGA CGTTCAGCTG 
GTCACGCAGC AGGTCATCCA GTGTGCGTAC GACATCGCCA AGGCTGCCAA GCAGCTGGTT 
ACCATCACCA CCAAAGAGAA CAACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( XM_017020258.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875747.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu84344
Clone ID Related Accession (Same CDS sequence)	XM_017020258.1 , XM_017020258.2
Accession Version	XM_017020258.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCCTGGA TTTGTCTGAA 
TTGGCAAAAG CTGCTAAGAA GAAACTTCAA TCTCTAAGTA ATCATTTGTT TGAAGAACTT 
GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACGG ATGCAGTCTG GCTTGCCACG 
CAAAACCACA GCGCCCTGGT AACCGAGACA ACGGTCGTCC CCTTTCTTCC GGTCAATCCT 
GAGTACTCAT CAACACGAAA TCAGGGCAGA CAGAAGTTAG CTCGGTTCAA CGCCCATGAG 
TTTGCCACGC TGGTCATTGA CATTCTCAGT GACGCCAAGA GGAGACAGCA GGGCAGTTCT 
CTCTCGGGTT CAAAAGACAA TGTGGAGCTC ATACTGAAAA CCATCAATAA CCAGCACAGC 
GTTGAGAGTC AAGACAACGA TCAGCCCGAC TATGACAGCG TGGCATCAGA CGAAGACACA 
GATTTGGAAA CCACTGCAAG CAAAACAAAC CGGCAGAAGA GCCTAGATTC AGATTTATCA 
GATGGACCAG TCACTGTACA GGAATTTATG GAGGTCAAAA ACGCTCTAGT GGCTTCTGAG 
GCCAAGATAC AGCAGCTAAT GAAGCTTCAA ACACTCCAGA GTGAAAATTC GAACCTCAGG 
AAACAGGCCA CAACCAATGT ATATCAGGTG CAAACTGGTT CTGAGTACAC AGACACTTCC 
AACCACTCTT CCTTAAAGAG ACGTCCGTCT GCCCGGGGCA GTAGGCCCAT GTCCATGTAC 
GAGACCGGAT CAGGTCAGAA ACCATATCTC CCAATGGGAG AAGCGAGCCG CCCCGAAGAG 
AGCAGGATGA GACTCCAGCC CTTCCCCGCG CACGCATCCA GGCTGGAGAA GCAGAACAGC 
ACACCTGAGA GTGACTACGA CAACACTCCC AACGACATGG AGCCAGATGG CATGGGGTCA 
AGCCGAAAGG GACGGCAAAG AAGTATGGTG TGGCCAGGGG ATGGCTTGGT ACCAGACACA 
GCAGAACCCC ATGTGGCCCC AAGCCCCACT CTCCCTAGCA CCGAAGATGT CATCAGGAAG 
ACTGAACAGA TCACCAAAAA CATACAGGAG CTCTTAAGAG CAGCCCAAGA AAATAAACAT 
GACAGTTATA TTCCCTGCTC AGAGAGGATA CACGTAGCTG TTACAGAAAT GGCAGCATTA 
TTCCCCAAAA AACCCAAGTC TGATATGGTG AGGACTTCCC TTCGTTTACT GACGTCCAGT 
GCCTACCGAC TGCAGTCAGA GTGCAAGAAG ACCCTCCCAG GGGACCCCGG CTCACCCACA 
GACGTTCAGC TGGTCACGCA GCAGGTCATC CAGTGTGCGT ACGACATCGC CAAGGCTGCC 
AAGCAGCTGG TTACCATCAC CACCAAAGAG AACAACAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875747.1
CDS	161..2302
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Target ORF information:

RefSeq Version	XM_017020258.1
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020258.1

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCCTGGA TTTGTCTGAA 
TTGGCAAAAG CTGCTAAGAA GAAACTTCAA TCTCTAAGTA ATCATTTGTT TGAAGAACTT 
GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACGG ATGCAGTCTG GCTTGCCACG 
CAAAACCACA GCGCCCTGGT AACCGAGACA ACGGTCGTCC CCTTTCTTCC GGTCAATCCT 
GAGTACTCAT CAACACGAAA TCAGGGCAGA CAGAAGTTAG CTCGGTTCAA CGCCCATGAG 
TTTGCCACGC TGGTCATTGA CATTCTCAGT GACGCCAAGA GGAGACAGCA GGGCAGTTCT 
CTCTCGGGTT CAAAAGACAA TGTGGAGCTC ATACTGAAAA CCATCAATAA CCAGCACAGC 
GTTGAGAGTC AAGACAACGA TCAGCCCGAC TATGACAGCG TGGCATCAGA CGAAGACACA 
GATTTGGAAA CCACTGCAAG CAAAACAAAC CGGCAGAAGA GCCTAGATTC AGATTTATCA 
GATGGACCAG TCACTGTACA GGAATTTATG GAGGTCAAAA ACGCTCTAGT GGCTTCTGAG 
GCCAAGATAC AGCAGCTAAT GAAGCTTCAA ACACTCCAGA GTGAAAATTC GAACCTCAGG 
AAACAGGCCA CAACCAATGT ATATCAGGTG CAAACTGGTT CTGAGTACAC AGACACTTCC 
AACCACTCTT CCTTAAAGAG ACGTCCGTCT GCCCGGGGCA GTAGGCCCAT GTCCATGTAC 
GAGACCGGAT CAGGTCAGAA ACCATATCTC CCAATGGGAG AAGCGAGCCG CCCCGAAGAG 
AGCAGGATGA GACTCCAGCC CTTCCCCGCG CACGCATCCA GGCTGGAGAA GCAGAACAGC 
ACACCTGAGA GTGACTACGA CAACACTCCC AACGACATGG AGCCAGATGG CATGGGGTCA 
AGCCGAAAGG GACGGCAAAG AAGTATGGTG TGGCCAGGGG ATGGCTTGGT ACCAGACACA 
GCAGAACCCC ATGTGGCCCC AAGCCCCACT CTCCCTAGCA CCGAAGATGT CATCAGGAAG 
ACTGAACAGA TCACCAAAAA CATACAGGAG CTCTTAAGAG CAGCCCAAGA AAATAAACAT 
GACAGTTATA TTCCCTGCTC AGAGAGGATA CACGTAGCTG TTACAGAAAT GGCAGCATTA 
TTCCCCAAAA AACCCAAGTC TGATATGGTG AGGACTTCCC TTCGTTTACT GACGTCCAGT 
GCCTACCGAC TGCAGTCAGA GTGCAAGAAG ACCCTCCCAG GGGACCCCGG CTCACCCACA 
GACGTTCAGC TGGTCACGCA GCAGGTCATC CAGTGTGCGT ACGACATCGC CAAGGCTGCC 
AAGCAGCTGG TTACCATCAC CACCAAAGAG AACAACAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( XM_017020258.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875747.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu84344
Clone ID Related Accession (Same CDS sequence)	XM_017020258.1 , XM_017020258.2
Accession Version	XM_017020258.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017020258.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCCTGGA TTTGTCTGAA 
TTGGCAAAAG CTGCTAAGAA GAAACTTCAA TCTCTAAGTA ATCATTTGTT TGAAGAACTT 
GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACGG ATGCAGTCTG GCTTGCCACG 
CAAAACCACA GCGCCCTGGT AACCGAGACA ACGGTCGTCC CCTTTCTTCC GGTCAATCCT 
GAGTACTCAT CAACACGAAA TCAGGGCAGA CAGAAGTTAG CTCGGTTCAA CGCCCATGAG 
TTTGCCACGC TGGTCATTGA CATTCTCAGT GACGCCAAGA GGAGACAGCA GGGCAGTTCT 
CTCTCGGGTT CAAAAGACAA TGTGGAGCTC ATACTGAAAA CCATCAATAA CCAGCACAGC 
GTTGAGAGTC AAGACAACGA TCAGCCCGAC TATGACAGCG TGGCATCAGA CGAAGACACA 
GATTTGGAAA CCACTGCAAG CAAAACAAAC CGGCAGAAGA GCCTAGATTC AGATTTATCA 
GATGGACCAG TCACTGTACA GGAATTTATG GAGGTCAAAA ACGCTCTAGT GGCTTCTGAG 
GCCAAGATAC AGCAGCTAAT GAAGCTTCAA ACACTCCAGA GTGAAAATTC GAACCTCAGG 
AAACAGGCCA CAACCAATGT ATATCAGGTG CAAACTGGTT CTGAGTACAC AGACACTTCC 
AACCACTCTT CCTTAAAGAG ACGTCCGTCT GCCCGGGGCA GTAGGCCCAT GTCCATGTAC 
GAGACCGGAT CAGGTCAGAA ACCATATCTC CCAATGGGAG AAGCGAGCCG CCCCGAAGAG 
AGCAGGATGA GACTCCAGCC CTTCCCCGCG CACGCATCCA GGCTGGAGAA GCAGAACAGC 
ACACCTGAGA GTGACTACGA CAACACTCCC AACGACATGG AGCCAGATGG CATGGGGTCA 
AGCCGAAAGG GACGGCAAAG AAGTATGGTG TGGCCAGGGG ATGGCTTGGT ACCAGACACA 
GCAGAACCCC ATGTGGCCCC AAGCCCCACT CTCCCTAGCA CCGAAGATGT CATCAGGAAG 
ACTGAACAGA TCACCAAAAA CATACAGGAG CTCTTAAGAG CAGCCCAAGA AAATAAACAT 
GACAGTTATA TTCCCTGCTC AGAGAGGATA CACGTAGCTG TTACAGAAAT GGCAGCATTA 
TTCCCCAAAA AACCCAAGTC TGATATGGTG AGGACTTCCC TTCGTTTACT GACGTCCAGT 
GCCTACCGAC TGCAGTCAGA GTGCAAGAAG ACCCTCCCAG GGGACCCCGG CTCACCCACA 
GACGTTCAGC TGGTCACGCA GCAGGTCATC CAGTGTGCGT ACGACATCGC CAAGGCTGCC 
AAGCAGCTGG TTACCATCAC CACCAAAGAG AACAACAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875747.1
CDS	71..2212
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Target ORF information:

RefSeq Version	XM_017020258.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020258.2

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCCTGGA TTTGTCTGAA 
TTGGCAAAAG CTGCTAAGAA GAAACTTCAA TCTCTAAGTA ATCATTTGTT TGAAGAACTT 
GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACGG ATGCAGTCTG GCTTGCCACG 
CAAAACCACA GCGCCCTGGT AACCGAGACA ACGGTCGTCC CCTTTCTTCC GGTCAATCCT 
GAGTACTCAT CAACACGAAA TCAGGGCAGA CAGAAGTTAG CTCGGTTCAA CGCCCATGAG 
TTTGCCACGC TGGTCATTGA CATTCTCAGT GACGCCAAGA GGAGACAGCA GGGCAGTTCT 
CTCTCGGGTT CAAAAGACAA TGTGGAGCTC ATACTGAAAA CCATCAATAA CCAGCACAGC 
GTTGAGAGTC AAGACAACGA TCAGCCCGAC TATGACAGCG TGGCATCAGA CGAAGACACA 
GATTTGGAAA CCACTGCAAG CAAAACAAAC CGGCAGAAGA GCCTAGATTC AGATTTATCA 
GATGGACCAG TCACTGTACA GGAATTTATG GAGGTCAAAA ACGCTCTAGT GGCTTCTGAG 
GCCAAGATAC AGCAGCTAAT GAAGCTTCAA ACACTCCAGA GTGAAAATTC GAACCTCAGG 
AAACAGGCCA CAACCAATGT ATATCAGGTG CAAACTGGTT CTGAGTACAC AGACACTTCC 
AACCACTCTT CCTTAAAGAG ACGTCCGTCT GCCCGGGGCA GTAGGCCCAT GTCCATGTAC 
GAGACCGGAT CAGGTCAGAA ACCATATCTC CCAATGGGAG AAGCGAGCCG CCCCGAAGAG 
AGCAGGATGA GACTCCAGCC CTTCCCCGCG CACGCATCCA GGCTGGAGAA GCAGAACAGC 
ACACCTGAGA GTGACTACGA CAACACTCCC AACGACATGG AGCCAGATGG CATGGGGTCA 
AGCCGAAAGG GACGGCAAAG AAGTATGGTG TGGCCAGGGG ATGGCTTGGT ACCAGACACA 
GCAGAACCCC ATGTGGCCCC AAGCCCCACT CTCCCTAGCA CCGAAGATGT CATCAGGAAG 
ACTGAACAGA TCACCAAAAA CATACAGGAG CTCTTAAGAG CAGCCCAAGA AAATAAACAT 
GACAGTTATA TTCCCTGCTC AGAGAGGATA CACGTAGCTG TTACAGAAAT GGCAGCATTA 
TTCCCCAAAA AACCCAAGTC TGATATGGTG AGGACTTCCC TTCGTTTACT GACGTCCAGT 
GCCTACCGAC TGCAGTCAGA GTGCAAGAAG ACCCTCCCAG GGGACCCCGG CTCACCCACA 
GACGTTCAGC TGGTCACGCA GCAGGTCATC CAGTGTGCGT ACGACATCGC CAAGGCTGCC 
AAGCAGCTGG TTACCATCAC CACCAAAGAG AACAACAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( XM_017020259.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875748.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu84345
Clone ID Related Accession (Same CDS sequence)	XM_017020259.2 , XM_017020259.1
Accession Version	XM_017020259.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017020259.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT 
ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA 
ACCAATGTAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC 
TTAAAGAGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACCGGATCA 
GGTCAGAAAC CATATCTCCC AATGGGAGAA GCGAGCCGCC CCGAAGAGAG CAGGATGAGA 
CTCCAGCCCT TCCCCGCGCA CATCGGGAGG AGTGCACTTG TGACCTCCTC TTCATCTCTG 
CCTTCCTTCC CCTCCACACT TTCCTGGTCG AGGGACGAAA GCGCCCGAAG GGCATCCAGG 
CTGGAGAAGC AGAACAGCAC ACCTGAGAGT GACTACGACA ACACTCCCAA CGACATGGAG 
CCAGATGGCA TGGGGTCAAG CCGAAAGGGA CGGCAAAGAA GTATGGTGTG GCCAGGGGAT 
GGCTTGGTAC CAGACACAGC AGAACCCCAT GTGGCCCCAA GCCCCACTCT CCCTAGCACC 
GAAGATGTCA TCAGGAAGAC TGAACAGATC ACCAAAAACA TACAGGAGCT CTTAAGAGCA 
GCCCAAGAAA ATAAACATGA CAGTTATATT CCCTGCTCAG AGAGGATACA CGTAGCTGTT 
ACAGAAATGG CAGCATTATT CCCCAAAGTA AAAACCCAAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875748.1
CDS	71..2155
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKVNNNLSDELRIMQKKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHIGRSALVTSSSSLPSFPSTLSWSRDESARRASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKVKTQV

Target ORF information:

RefSeq Version	XM_017020259.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020259.2

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT 
ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA 
ACCAATGTAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC 
TTAAAGAGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACCGGATCA 
GGTCAGAAAC CATATCTCCC AATGGGAGAA GCGAGCCGCC CCGAAGAGAG CAGGATGAGA 
CTCCAGCCCT TCCCCGCGCA CATCGGGAGG AGTGCACTTG TGACCTCCTC TTCATCTCTG 
CCTTCCTTCC CCTCCACACT TTCCTGGTCG AGGGACGAAA GCGCCCGAAG GGCATCCAGG 
CTGGAGAAGC AGAACAGCAC ACCTGAGAGT GACTACGACA ACACTCCCAA CGACATGGAG 
CCAGATGGCA TGGGGTCAAG CCGAAAGGGA CGGCAAAGAA GTATGGTGTG GCCAGGGGAT 
GGCTTGGTAC CAGACACAGC AGAACCCCAT GTGGCCCCAA GCCCCACTCT CCCTAGCACC 
GAAGATGTCA TCAGGAAGAC TGAACAGATC ACCAAAAACA TACAGGAGCT CTTAAGAGCA 
GCCCAAGAAA ATAAACATGA CAGTTATATT CCCTGCTCAG AGAGGATACA CGTAGCTGTT 
ACAGAAATGG CAGCATTATT CCCCAAAGTA AAAACCCAAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( XM_017020259.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875748.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu84345
Clone ID Related Accession (Same CDS sequence)	XM_017020259.2 , XM_017020259.1
Accession Version	XM_017020259.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT 
ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA 
ACCAATGTAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC 
TTAAAGAGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACCGGATCA 
GGTCAGAAAC CATATCTCCC AATGGGAGAA GCGAGCCGCC CCGAAGAGAG CAGGATGAGA 
CTCCAGCCCT TCCCCGCGCA CATCGGGAGG AGTGCACTTG TGACCTCCTC TTCATCTCTG 
CCTTCCTTCC CCTCCACACT TTCCTGGTCG AGGGACGAAA GCGCCCGAAG GGCATCCAGG 
CTGGAGAAGC AGAACAGCAC ACCTGAGAGT GACTACGACA ACACTCCCAA CGACATGGAG 
CCAGATGGCA TGGGGTCAAG CCGAAAGGGA CGGCAAAGAA GTATGGTGTG GCCAGGGGAT 
GGCTTGGTAC CAGACACAGC AGAACCCCAT GTGGCCCCAA GCCCCACTCT CCCTAGCACC 
GAAGATGTCA TCAGGAAGAC TGAACAGATC ACCAAAAACA TACAGGAGCT CTTAAGAGCA 
GCCCAAGAAA ATAAACATGA CAGTTATATT CCCTGCTCAG AGAGGATACA CGTAGCTGTT 
ACAGAAATGG CAGCATTATT CCCCAAAGTA AAAACCCAAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875748.1
CDS	158..2242
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKVNNNLSDELRIMQKKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHIGRSALVTSSSSLPSFPSTLSWSRDESARRASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKVKTQV

Target ORF information:

RefSeq Version	XM_017020259.1
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020259.1

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT 
ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA 
ACCAATGTAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC 
TTAAAGAGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACCGGATCA 
GGTCAGAAAC CATATCTCCC AATGGGAGAA GCGAGCCGCC CCGAAGAGAG CAGGATGAGA 
CTCCAGCCCT TCCCCGCGCA CATCGGGAGG AGTGCACTTG TGACCTCCTC TTCATCTCTG 
CCTTCCTTCC CCTCCACACT TTCCTGGTCG AGGGACGAAA GCGCCCGAAG GGCATCCAGG 
CTGGAGAAGC AGAACAGCAC ACCTGAGAGT GACTACGACA ACACTCCCAA CGACATGGAG 
CCAGATGGCA TGGGGTCAAG CCGAAAGGGA CGGCAAAGAA GTATGGTGTG GCCAGGGGAT 
GGCTTGGTAC CAGACACAGC AGAACCCCAT GTGGCCCCAA GCCCCACTCT CCCTAGCACC 
GAAGATGTCA TCAGGAAGAC TGAACAGATC ACCAAAAACA TACAGGAGCT CTTAAGAGCA 
GCCCAAGAAA ATAAACATGA CAGTTATATT CCCTGCTCAG AGAGGATACA CGTAGCTGTT 
ACAGAAATGG CAGCATTATT CCCCAAAGTA AAAACCCAAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( XM_017020260.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875749.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X8, mRNA.

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CloneID	OHu84346
Clone ID Related Accession (Same CDS sequence)	XM_017020260.1 , XM_017020260.2
Accession Version	XM_017020260.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT 
ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA 
ACCAATGTAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC 
TTAAAGAGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACCGGATCA 
GGTCAGAAAC CATATCTCCC AATGGGAGAA GCGAGCCGCC CCGAAGAGAG CAGGATGAGA 
CTCCAGCCCT TCCCCGCGCA CGCATCCAGG CTGGAGAAGC AGAACAGCAC ACCTGAGAGT 
GACTACGACA ACACTCCCAA CGACATGGAG CCAGATGGCA TGGGGTCAAG CCGAAAGGGA 
CGGCAAAGAA GTATGGTGTG GCCAGGGGAT GGCTTGGTAC CAGACACAGC AGAACCCCAT 
GTGGCCCCAA GCCCCACTCT CCCTAGCACC GAAGATGTCA TCAGGAAGAC TGAACAGATC 
ACCAAAAACA TACAGGAGCT CTTAAGAGCA GCCCAAGAAA ATAAACATGA CAGTTATATT 
CCCTGCTCAG AGAGGATACA CGTAGCTGTT ACAGAAATGG CAGCATTATT CCCCAAAGTA 
AAAACCCAAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875749.1
CDS	158..2152
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKVNNNLSDELRIMQKKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKVKTQV

Target ORF information:

RefSeq Version	XM_017020260.1
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020260.1

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT 
ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA 
ACCAATGTAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC 
TTAAAGAGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACCGGATCA 
GGTCAGAAAC CATATCTCCC AATGGGAGAA GCGAGCCGCC CCGAAGAGAG CAGGATGAGA 
CTCCAGCCCT TCCCCGCGCA CGCATCCAGG CTGGAGAAGC AGAACAGCAC ACCTGAGAGT 
GACTACGACA ACACTCCCAA CGACATGGAG CCAGATGGCA TGGGGTCAAG CCGAAAGGGA 
CGGCAAAGAA GTATGGTGTG GCCAGGGGAT GGCTTGGTAC CAGACACAGC AGAACCCCAT 
GTGGCCCCAA GCCCCACTCT CCCTAGCACC GAAGATGTCA TCAGGAAGAC TGAACAGATC 
ACCAAAAACA TACAGGAGCT CTTAAGAGCA GCCCAAGAAA ATAAACATGA CAGTTATATT 
CCCTGCTCAG AGAGGATACA CGTAGCTGTT ACAGAAATGG CAGCATTATT CCCCAAAGTA 
AAAACCCAAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIT2 ( XM_017020260.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875749.1 Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu84346
Clone ID Related Accession (Same CDS sequence)	XM_017020260.1 , XM_017020260.2
Accession Version	XM_017020260.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ARF GTPase-activating protein GIT2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017020260.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT 
ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA 
ACCAATGTAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC 
TTAAAGAGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACCGGATCA 
GGTCAGAAAC CATATCTCCC AATGGGAGAA GCGAGCCGCC CCGAAGAGAG CAGGATGAGA 
CTCCAGCCCT TCCCCGCGCA CGCATCCAGG CTGGAGAAGC AGAACAGCAC ACCTGAGAGT 
GACTACGACA ACACTCCCAA CGACATGGAG CCAGATGGCA TGGGGTCAAG CCGAAAGGGA 
CGGCAAAGAA GTATGGTGTG GCCAGGGGAT GGCTTGGTAC CAGACACAGC AGAACCCCAT 
GTGGCCCCAA GCCCCACTCT CCCTAGCACC GAAGATGTCA TCAGGAAGAC TGAACAGATC 
ACCAAAAACA TACAGGAGCT CTTAAGAGCA GCCCAAGAAA ATAAACATGA CAGTTATATT 
CCCTGCTCAG AGAGGATACA CGTAGCTGTT ACAGAAATGG CAGCATTATT CCCCAAAGTA 
AAAACCCAAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875749.1
CDS	71..2065
Translation	MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKVNNNLSDELRIMQKKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKVKTQV

Target ORF information:

RefSeq Version	XM_017020260.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020260.2

ORF Insert Sequence:

ATGTCGAAAC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
TGGGCATCAG TAAATAGGGG AACGTTTTTA TGTGATGAGT GCTGCAGTGT CCATCGGAGT 
CTAGGGCGCC ATATCTCCCA AGTGAGGCAT CTGAAACACA CACCGTGGCC TCCAACACTG 
CTTCAGATGG TTGAGACCTT GTATAATAAC GGTGCTAACT CTATATGGGA GCATTCTTTG 
CTGGACCCTG CGTCTATTAT GAGTGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT 
CCCAATAAAG CGGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCATCGCTTG 
CCCTGCCGGG ATGACGATAG TGTGACTGCC AAAGATCTTA GCAAGCAACT CCATTCGAGC 
GTGAGAACAG GGAATCTTGA AACCTGTTTG AGACTGTTAT CTTTAGGAGC ACAAGCCAAC 
TTCTTTCATC CTGAAAAAGG AAACACCCCA CTCCATGTTG CCTCCAAAGC AGGGCAGATT 
TTACAGGCTG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGG 
AAAACTCCCG TTGATTATGC AAGGCAAGGA GGGCACCATG AGCTGGCAGA GCGCCTCGTG 
GAAATACAGT ATGAGCTAAC GGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT 
CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT 
GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAATCTCTAA GTAATCATTT GTTTGAAGAA 
CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CGGATGCAGT CTGGCTTGCC 
ACGCAAAACC ACAGCGCCCT GGTAACCGAG ACAACGGTCG TCCCCTTTCT TCCGGTCAAT 
CCTGAGTACT CATCAACACG AAATCAGGGC AGACAGAAGT TAGCTCGGTT CAACGCCCAT 
GAGTTTGCCA CGCTGGTCAT TGACATTCTC AGTGACGCCA AGAGGAGACA GCAGGGCAGT 
TCTCTCTCGG GTTCAAAAGA CAATGTGGAG CTCATACTGA AAACCATCAA TAACCAGCAC 
AGCGTTGAGA GTCAAGACAA CGATCAGCCC GACTATGACA GCGTGGCATC AGACGAAGAC 
ACAGATTTGG AAACCACTGC AAGCAAAACA AACCGGCAGA AGAGCCTAGA TTCAGATTTA 
TCAGATGGAC CAGTCACTGT ACAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT 
GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT 
ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA 
ACCAATGTAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC 
TTAAAGAGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACCGGATCA 
GGTCAGAAAC CATATCTCCC AATGGGAGAA GCGAGCCGCC CCGAAGAGAG CAGGATGAGA 
CTCCAGCCCT TCCCCGCGCA CGCATCCAGG CTGGAGAAGC AGAACAGCAC ACCTGAGAGT 
GACTACGACA ACACTCCCAA CGACATGGAG CCAGATGGCA TGGGGTCAAG CCGAAAGGGA 
CGGCAAAGAA GTATGGTGTG GCCAGGGGAT GGCTTGGTAC CAGACACAGC AGAACCCCAT 
GTGGCCCCAA GCCCCACTCT CCCTAGCACC GAAGATGTCA TCAGGAAGAC TGAACAGATC 
ACCAAAAACA TACAGGAGCT CTTAAGAGCA GCCCAAGAAA ATAAACATGA CAGTTATATT 
CCCTGCTCAG AGAGGATACA CGTAGCTGTT ACAGAAATGG CAGCATTATT CCCCAAAGTA 
AAAACCCAAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The focal adhesion-associated proteins DOCK5 and GIT2 comprise a rheostat in control of epithelial invasion.
Oncogene36(13)1816-1828(2017 Mar)
Frank SR,K?llmann CP,van Lidth de Jeude JF,Thiagarajah JR,Engelholm LH,Fr?din M,Hansen SH

EGF-stimulated activation of Rab35 regulates RUSC2-GIT2 complex formation to stabilize GIT2 during directional lung cancer cell migration.
Cancer letters379(1)70-83(2016 08)
Duan B,Cui J,Sun S,Zheng J,Zhang Y,Ye B,Chen Y,Deng W,Du J,Zhu Y,Chen Y,Gu L

Expanding functions of GIT Arf GTPase-activating proteins, PIX Rho guanine nucleotide exchange factors and GIT-PIX complexes.
Journal of cell science129(10)1963-74(2016 05)
Zhou W,Li X,Premont RT

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M

Nuclear GIT2 is an ATM substrate and promotes DNA repair.
Molecular and cellular biology35(7)1081-96(2015 Apr)
Lu D,Cai H,Park SS,Siddiqui S,Premont RT,Schmalzigaug R,Paramasivam M,Seidman M,Bodogai I,Biragyn A,Daimon CM,Martin B,Maudsley S

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GIT2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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