Git cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Git
Entrez Gene ID	36122
Full Name	G protein-coupled receptor kinase interacting ArfGAP
Synonyms	CG16728,CT37209,Dmel\CG16728,GIT,arfgap2,dGit,dgit,git
General protein information	Preferred Names G protein-coupled receptor kinase interacting ArfGAP Names CG16728 gene product from transcript CG16728-RA CG16728-PA G-protein-coupled receptor kinase-interacting protein Git-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 47B1-47B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_136755.4	NP_610599.3	G protein-coupled receptor kinase interacting ArfGAP

Pathways from BioSystems

Homologies

Homo sapiens (human)	GIT2	NP_001128686.1
Canis lupus familiaris (dog)	GIT2	XP_005636373.1
Rattus norvegicus (Norway rat)	Git2	NP_001005553.1
Danio rerio (zebrafish)	git2a	NP_001119925.1
Bos taurus (cattle)	GIT2	NP_001180030.1
Drosophila melanogaster (fruit fly)	Git	NP_610599.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006888	XP_308871.4
	GIT2	XP_001106038.1
Gallus gallus (chicken)	GIT2	NP_989537.1
Pan troglodytes (chimpanzee)	GIT2	XP_001139823.2
Mus musculus (house mouse)	Git2	NP_001070827.1
Xenopus tropicalis (tropical clawed frog)	git2	NP_001016917.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pix and Git regulate Hippo-pathway-dependent tissue growth.
Title: The GTPase regulatory proteins Pix and Git control tissue growth via the Hippo pathway.

GIT regulates stoned B levels and synaptic vesicle recycling.
Title: A presynaptic role for the cytomatrix protein GIT in synaptic vesicle recycling.

dGIT (CG16728) and dPak are part of a complex that promotes proper muscle morphogenesis and myotube targeting during embryogenesis.
Title: The Drosophila homologue of Arf-GAP GIT1, dGIT, is required for proper muscle morphogenesis and guidance during embryogenesis.

The following Git gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Git cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12899	NM_136755.4 Latest version!	Drosophila melanogaster G protein-coupled receptor kinase interacting ArfGAP (Git), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Git ( NM_136755.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610599.3 Drosophila melanogaster G protein-coupled receptor kinase interacting ArfGAP (Git), mRNA.

Price & Availability↑

CloneID	OFa12899
Clone ID Related Accession (Same CDS sequence)	NM_136755.4
Accession Version	NM_136755.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	G protein-coupled receptor kinase interacting ArfGAP
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136755.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGTTTCG CCAGCAGTAT AATCGAAGCG CACAGGAAGT TATTTATAGC GCCGCCGGAT 
TTGGATCATT CCGATCCAGC CACACCCACG ATTTCGACCA GATCCAAAAT GCCGAGGGGG 
AAATCCCGCC TGCAAACCGA AGTTTGTGGC GATTGCGGAG CTGGTGATCC ATCCTGGGCG 
AGCATCAACC GGGGCATCCT TCTCTGCGCC GACTGCTGTT CGGTGCACCG CAGTCTGGGC 
AGGCACATCT CCATTGTGAA GTCTCTACGC CAGGGTAACT GGGAGCCCTC GGTTCTGAAC 
TTTGTGAACT CTCTAAACGC CCATGGAGCC AATAGTGTTT GGGAGCATCA TCTGCTGGAT 
GGCAGCACCA ACTCCACCGG CGGAAAGCAC GTGCCACGCT GGCGGAAACC CACGCCCAAG 
GATGCACTCC ATCCCACCAA ATCCGATTTT ATTAAGGCCA AGCATGTGAA TCTAACGTTC 
GTGCTAAAAC CCAGTCTCCA GGATGACGAT GATGGCAATG GCTCAGCGGG CTGTCTGGAA 
CAGGAGCTGA GTAGACAGCT CCATGCCAGC GTGCGGACCA GCAATCTAGA GACTTCACTG 
CGCTTCTTGG TCCAGGGTGC AGACCCAAAT TACTACCACG AGGACAAGCT ATCAACGCCA 
CTTCACATGG CCGCCAAGTT TGGCCAGGCA TCACAAATCG AGATGCTGCT CATCTATGGA 
GCCGATGTGA ATGCCTTGGA TGGCAATGGG ATGACTCCTC TGGAACTGGC CAGGGCCAAC 
AATCATAACA CCATCGCCGA AAGACTGCTT GATGCCATGT ACGATGTCAC CGACAGGATC 
ATTACCTTCC TAGGTGGCAA GAAGCCAGAT CATGCGAGTG GCCGCCACAT GATTATTCCG 
GATGCCAATG GCGCGGACAT AAGCGAGCAA CTGAAGATTG CACGTGGAAA GTTGCAACTC 
GTGCCAAATA AGATGTTTGA GGAGCTGGTC ATGGACCTAT ACGATGAGGT GGACAGACGC 
GAGTGCGAAG CCATCTGGTC GACAAGCACC CTGAATGCTG ATCATGCAAC TGTGCCATTT 
TTGCCGGCTA ATCCATTTTT GAGCGCCACG CGCAATCAGG GTCGTCAGAA ATTGGCACGC 
TTCAATCGCG CCGAGTTCAC TGGTCTCTTG ACCGACGTGC TCGTCGATGC TATGCGACGC 
CAGAATATGG CCAACCTGCG TCCAATGGAT GCCCCAGTGG CCGGGCACCA GTCGCTGCAA 
TCGCTGCCGT ATGCCAACAA CTCAATGCTG CTCGGCTCGT TCGAGCAGGG CGGTCATGAT 
CCGAATCTGT CGGACGACGA GCCCATTTAT GATCCGGTGG CCAGCGATGA CGACTATGCC 
CCAGTGCCAC CAATGGCCCA GCAGGCCATT GTGCATACGC CACCGCGCAG CGCGAATTCG 
CACAACGAAA TGGAAACGCT GCGCAAACAG CTGAACGATT ACAAATCGGA GATCAATCAG 
CTAAAGAACG TGGTGCAGAT GCTGTCCAGC GAGAACACGC AGCTCAAGTC CAAGTTCTCG 
AGCGCATCGA ACAACAGCGT GTACGATGAG CCACTTCGCA TTGATCTGAG CTTGAGCAGT 
CCGGATACAG AGCACGAGCC TCTGTCGCTA CCCGAAGGTG GGACTGCCAA TGGGGAAAGC 
GGCTCCTCCA ATGATTCGTC AAATCAATCG ACCATCAAGC GTCCAGCCAG CATGTACGAG 
CGTCGTTTGG TACCCAATGT GGCCAAGGGC AATACGGACA TACGAAATAC GACCAGCATG 
TACCAAATGG CTGGGGATGG CAAACCTTTT GGTGAGGAGG TCAAAGTGCG CTCTGACTTG 
GTCACACGCC GTCTTAAGGA GCTCATAAGA GCTATGCAGC CAGTGCCAGA GGATCAAAAG 
CAGTCGATTG CTCCACATGG AGAGCTTATA CGAAGTGCCG TGACTGATTT AATTGCCCTT 
TATGCCAATT TGCCACCGAA TGCCAGTGAC CCGTCTCGCG AAACCCTTAA ACTGCTAACC 
CGCCAGAACA TTCTCATCCA GCACGAGTGC GAGAATCTGC AGAAAGCCAT CGAGGCGGAT 
GACAAGCAGG CCATACAGAA GAACACCTTG GAGGTGCGCG ACTGCGCCTT CCACATTGCC 
AGTGCCATAA AGACTTTGGT GTTGCAGTTC TATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610599.3
CDS	115..2310
Translation	MCFASSIIEAHRKLFIAPPDLDHSDPATPTISTRSKMPRGKSRLQTEVCGDCGAGDPSWASINRGILLCADCCSVHRSLGRHISIVKSLRQGNWEPSVLNFVNSLNAHGANSVWEHHLLDGSTNSTGGKHVPRWRKPTPKDALHPTKSDFIKAKHVNLTFVLKPSLQDDDDGNGSAGCLEQELSRQLHASVRTSNLETSLRFLVQGADPNYYHEDKLSTPLHMAAKFGQASQIEMLLIYGADVNALDGNGMTPLELARANNHNTIAERLLDAMYDVTDRIITFLGGKKPDHASGRHMIIPDANGADISEQLKIARGKLQLVPNKMFEELVMDLYDEVDRRECEAIWSTSTLNADHATVPFLPANPFLSATRNQGRQKLARFNRAEFTGLLTDVLVDAMRRQNMANLRPMDAPVAGHQSLQSLPYANNSMLLGSFEQGGHDPNLSDDEPIYDPVASDDDYAPVPPMAQQAIVHTPPRSANSHNEMETLRKQLNDYKSEINQLKNVVQMLSSENTQLKSKFSSASNNSVYDEPLRIDLSLSSPDTEHEPLSLPEGGTANGESGSSNDSSNQSTIKRPASMYERRLVPNVAKGNTDIRNTTSMYQMAGDGKPFGEEVKVRSDLVTRRLKELIRAMQPVPEDQKQSIAPHGELIRSAVTDLIALYANLPPNASDPSRETLKLLTRQNILIQHECENLQKAIEADDKQAIQKNTLEVRDCAFHIASAIKTLVLQFY

Target ORF information:

RefSeq Version	NM_136755.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster G protein-coupled receptor kinase interacting ArfGAP (Git), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136755.4

ORF Insert Sequence:

ATGTGTTTCG CCAGCAGTAT AATCGAAGCG CACAGGAAGT TATTTATAGC GCCGCCGGAT 
TTGGATCATT CCGATCCAGC CACACCCACG ATTTCGACCA GATCCAAAAT GCCGAGGGGG 
AAATCCCGCC TGCAAACCGA AGTTTGTGGC GATTGCGGAG CTGGTGATCC ATCCTGGGCG 
AGCATCAACC GGGGCATCCT TCTCTGCGCC GACTGCTGTT CGGTGCACCG CAGTCTGGGC 
AGGCACATCT CCATTGTGAA GTCTCTACGC CAGGGTAACT GGGAGCCCTC GGTTCTGAAC 
TTTGTGAACT CTCTAAACGC CCATGGAGCC AATAGTGTTT GGGAGCATCA TCTGCTGGAT 
GGCAGCACCA ACTCCACCGG CGGAAAGCAC GTGCCACGCT GGCGGAAACC CACGCCCAAG 
GATGCACTCC ATCCCACCAA ATCCGATTTT ATTAAGGCCA AGCATGTGAA TCTAACGTTC 
GTGCTAAAAC CCAGTCTCCA GGATGACGAT GATGGCAATG GCTCAGCGGG CTGTCTGGAA 
CAGGAGCTGA GTAGACAGCT CCATGCCAGC GTGCGGACCA GCAATCTAGA GACTTCACTG 
CGCTTCTTGG TCCAGGGTGC AGACCCAAAT TACTACCACG AGGACAAGCT ATCAACGCCA 
CTTCACATGG CCGCCAAGTT TGGCCAGGCA TCACAAATCG AGATGCTGCT CATCTATGGA 
GCCGATGTGA ATGCCTTGGA TGGCAATGGG ATGACTCCTC TGGAACTGGC CAGGGCCAAC 
AATCATAACA CCATCGCCGA AAGACTGCTT GATGCCATGT ACGATGTCAC CGACAGGATC 
ATTACCTTCC TAGGTGGCAA GAAGCCAGAT CATGCGAGTG GCCGCCACAT GATTATTCCG 
GATGCCAATG GCGCGGACAT AAGCGAGCAA CTGAAGATTG CACGTGGAAA GTTGCAACTC 
GTGCCAAATA AGATGTTTGA GGAGCTGGTC ATGGACCTAT ACGATGAGGT GGACAGACGC 
GAGTGCGAAG CCATCTGGTC GACAAGCACC CTGAATGCTG ATCATGCAAC TGTGCCATTT 
TTGCCGGCTA ATCCATTTTT GAGCGCCACG CGCAATCAGG GTCGTCAGAA ATTGGCACGC 
TTCAATCGCG CCGAGTTCAC TGGTCTCTTG ACCGACGTGC TCGTCGATGC TATGCGACGC 
CAGAATATGG CCAACCTGCG TCCAATGGAT GCCCCAGTGG CCGGGCACCA GTCGCTGCAA 
TCGCTGCCGT ATGCCAACAA CTCAATGCTG CTCGGCTCGT TCGAGCAGGG CGGTCATGAT 
CCGAATCTGT CGGACGACGA GCCCATTTAT GATCCGGTGG CCAGCGATGA CGACTATGCC 
CCAGTGCCAC CAATGGCCCA GCAGGCCATT GTGCATACGC CACCGCGCAG CGCGAATTCG 
CACAACGAAA TGGAAACGCT GCGCAAACAG CTGAACGATT ACAAATCGGA GATCAATCAG 
CTAAAGAACG TGGTGCAGAT GCTGTCCAGC GAGAACACGC AGCTCAAGTC CAAGTTCTCG 
AGCGCATCGA ACAACAGCGT GTACGATGAG CCACTTCGCA TTGATCTGAG CTTGAGCAGT 
CCGGATACAG AGCACGAGCC TCTGTCGCTA CCCGAAGGTG GGACTGCCAA TGGGGAAAGC 
GGCTCCTCCA ATGATTCGTC AAATCAATCG ACCATCAAGC GTCCAGCCAG CATGTACGAG 
CGTCGTTTGG TACCCAATGT GGCCAAGGGC AATACGGACA TACGAAATAC GACCAGCATG 
TACCAAATGG CTGGGGATGG CAAACCTTTT GGTGAGGAGG TCAAAGTGCG CTCTGACTTG 
GTCACACGCC GTCTTAAGGA GCTCATAAGA GCTATGCAGC CAGTGCCAGA GGATCAAAAG 
CAGTCGATTG CTCCACATGG AGAGCTTATA CGAAGTGCCG TGACTGATTT AATTGCCCTT 
TATGCCAATT TGCCACCGAA TGCCAGTGAC CCGTCTCGCG AAACCCTTAA ACTGCTAACC 
CGCCAGAACA TTCTCATCCA GCACGAGTGC GAGAATCTGC AGAAAGCCAT CGAGGCGGAT 
GACAAGCAGG CCATACAGAA GAACACCTTG GAGGTGCGCG ACTGCGCCTT CCACATTGCC 
AGTGCCATAA AGACTTTGGT GTTGCAGTTC TATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Git cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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