PHLDB2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PHLDB2
Entrez Gene ID	90102
Full Name	pleckstrin homology like domain family B member 2
Synonyms	LL5b,LL5beta
General protein information	Preferred Names pleckstrin homology like domain family B member 2 Names pleckstrin homology-like domain family B member 2 LL5 beta protein LL5-beta
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q13.2
Disorder MIM:	610298

mRNA and Protein(s)

mRNA	Protein	Name
NM_001134437.2	NP_001127909.1	pleckstrin homology-like domain family B member 2 isoform b
NM_145753.2	NP_665696.1	pleckstrin homology-like domain family B member 2 isoform c
NM_001134439.1	NP_001127911.1	pleckstrin homology-like domain family B member 2 isoform a
NM_001134438.2	NP_001127910.1	pleckstrin homology-like domain family B member 2 isoform a
NM_001134438.1	NP_001127910.1	pleckstrin homology-like domain family B member 2 isoform a
NM_001134437.1	NP_001127909.1	pleckstrin homology-like domain family B member 2 isoform b
NM_001134439.2	NP_001127911.1	pleckstrin homology-like domain family B member 2 isoform a

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC570807	XP_005172794.1
Mus musculus (house mouse)	Phldb2	NP_001239371.1
Rattus norvegicus (Norway rat)	Phldb2	XP_006248361.1
Canis lupus familiaris (dog)	PHLDB2	XP_535736.4
Gallus gallus (chicken)	PHLDB2	XP_416632.4
Homo sapiens (human)	PHLDB2	NP_001127910.1
Pan troglodytes (chimpanzee)	PHLDB2	XP_001153603.2
	PHLDB2	XP_001097875.2
Bos taurus (cattle)	PHLDB2	NP_001193237.1
Danio rerio (zebrafish)	si:dkey-11p10.8	XP_005162816.1

Gene Ontology
Bibliography

Related articles in PubMed

p53 target miR-29c-3p suppresses colon cancer cell invasion and migration through inhibition of PHLDB2.
Chen G, Zhou T, Li Y, Yu Z, Sun L
Biochemical and biophysical research communications487(1)90-95(2017 05)

Prickle1 promotes focal adhesion disassembly in cooperation with the CLASP-LL5? complex in migrating cells.
Lim BC, Matsumoto S, Yamamoto H, Mizuno H, Kikuta J, Ishii M, Kikuchi A
Journal of cell science129(16)3115-29(2016 08)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S, Zaidel-Bar R
Science signaling7(354)rs7(2014 Dec)

Liprin-?1, ERC1 and LL5 define polarized and dynamic structures that are implicated in cell migration.
Astro V, Chiaretti S, Magistrati E, Fivaz M, de Curtis I
Journal of cell science127(Pt 17)3862-76(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Collectively, our findings present the first to elucidate that miR-29c is a direct p53 target gene, and also identify PHLDB2 as an important miR-29c target gene involved in colon cancer metastasis.
Title: p53 target miR-29c-3p suppresses colon cancer cell invasion and migration through inhibition of PHLDB2.

Prickle1 localized to the membrane through its farnesyl moiety, and the membrane localization was necessary for Prickle1 to regulate migration, to bind to CLASPs and LL5beta, and to promote microtubule targeting of focal adhesions.
Title: Prickle1 promotes focal adhesion disassembly in cooperation with the CLASP-LL5? complex in migrating cells.

Liprin-alpha1, ERC1a and LL5 also define new highly polarized and dynamic cytoplasmic structures uniquely localized near the protruding cell edge
Title: Liprin-?1, ERC1 and LL5 define polarized and dynamic structures that are implicated in cell migration.

These and other LL5beta-interacting proteins are associated with conventional podosomes in macrophages and podosome-like invadopodia in fibroblasts, strengthening the close relationship between synaptic and non-synaptic podosomes.
Title: Amotl2 interacts with LL5?, localizes to podosomes and regulates postsynaptic differentiation in muscle.

minor allele, A, intronic single nucleotide polymorphism 'rs951660'in PHLDB2 might induce a delayed splicing and increase the susceptibility to Vascular dementia
Title: Functions of intronic nucleotide variants in the gene encoding pleckstrin homology like domain beta 2 (PHLDB2) on susceptibility to vascular dementia.

The following PHLDB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHLDB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26090	NM_001134439.2 Latest version!	Homo sapiens pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu26090	NM_001134438.2 Latest version!	Homo sapiens pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu26394	NM_145753.2 Latest version!	Homo sapiens pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu27088	NM_001134437.2 Latest version!	Homo sapiens pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OHu26090	NM_001134438.1	Homo sapiens pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu27088	NM_001134437.1	Homo sapiens pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu26090	NM_001134439.1	Homo sapiens pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PHLDB2 ( NM_001134439.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001127911.1 Homo sapiens pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26090
Clone ID Related Accession (Same CDS sequence)	NM_001134439.1 , NM_001134438.2 , NM_001134438.1 , NM_001134439.2
Accession Version	NM_001134439.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Homo sapiens(human)
Product	pleckstrin homology-like domain family B member 2 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA272187.1, AB209903.1, AC060225.12 and AW264132.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209903.1, SRR1803616.36787.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGAGC ATAGCTACAT ACAAAAGGAG CTAGATTTAC AAAATGGTAG CTTAGAGGAA 
GACTCTGTGG TGCATTCTGT TGAGAACGAT TCCCAAAACA TGATGGAGAG CCTCAGCCCA 
AAGAAATACT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTATTCTGG CTCCTATTTA 
ACCCTCTCAC AACCTGTGCC TGCAAAGAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA 
AGCAGCCCCT CCTTAGCCAA AATCCAGGGA AGCAAGCAGT TCTCTTATGA TGGAACTGAC 
AAAAATATTC CTATGAAACC TCCAACTCCT TTACTCAACA CTACATCCTC CCTCAGTGGA 
TATCCACTTG GAAGAGCAGA CTTTGATCAT TATACTGGCC GGGACAGTGA AAGGGCCTTG 
AGGCTCTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGCATAAATC GCATGACAAT 
GTCTACTCTC TTGGAGGGCT GGAAGGTCGG AAGGCATCTG GCTCGCTCCT GGCCATGTGG 
AATGGAAGTT CCCTGAGTGA TGCTGGCCCG CCTCCTATCA GCAGATCGGG AGCCGCAAGC 
ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATGAGCATTC AGGACAGCCT GGCGCTTCAA 
CCCAAGTTAA CTAGACACAA GGAGCTTGCA TCTGAAAACA TCAATTTGAG AACTAGGAAG 
TACTCCAGCA GCAGCCTGAG TCACATGGGA GCCTACAGCC GATCACTTCC CAGGTTGTAC 
AGAGCCACAG AGAACCAGCT GACACCTCTC AGCTTGCCTC CAAGAAACTC TCTGGGCAAT 
TCCAAACGAA CAAAACTTGG GGAAAAGGAT CTACCTCATA GCGTAATAGA CAATGACAAT 
TACCTTAATT TTTCTTCTTT GAGCTCAGGG GCTTTACCCT ATAAAACCTC TGCTTCTGAA 
GGCAATCCTT ATGTAAGTTC TACCCTCAGT GTCCCTGCCA GTCCACGAGT GGCTCGGAAG 
ATGCTTCTGG CCTCCACCTC CTCCTGTGCC TCTGATGACT TTGATCAGGC TTCATATGTG 
GGGACAAACC CGAGTCATTC ACTTCTTGCT GGAGAGTCAG ACAGAGTTTT TGCGACCAGG 
AGGAACTTCT CTTGTGGATC TGTGGAATTT GATGAGGCAG ATTTGGAAAG CCTCAGACAG 
GCCTCAGGAA CCCCCCAGCC TGCCCTTCGG GAACGGAAAA GCAGTATTAG CTCCATTTCA 
GGACGTGATG ACCTGATGGA TTATCACCGG CGGCAGAGGG AGGAAAGACT CAGGGAGCAG 
GAAATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGTCTCTG TGCTGAATAC 
ACAAAGCCTG ACAGTCGCTT ATCTACTGGG ACCACCGTGG AAGATGTGCA GAAAATCAAC 
AAGGAGCTTG AGAAGCTGCA GCTCTCTGAT GAGGAGTCTG TGTTTGAGGA AGCCCTCATG 
AGCCCTGACA CAAGATACAG GTGCCACCGG AAAGACTCCC TCCCTGATGC AGACTTGGCA 
AGCTGTGGGA GTCTCAGTCA GAGCAGTGCC AGCTTCTTTA CCCCCAGGAG CACCAGGAAT 
GATGAACTAC TCAGTGACCT CACCCGGACT CCTCCACCAC CATCCTCCAC CTTTCCGAAA 
GCTTCCAGCG AGTCCTCTTA TCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA 
GAACAGAGAT CTCAGGAGTT GGCTGCAATG GAAGAAACCC GGATAGTCAT TCTGAACAAC 
CTCGAGGAAC TTAAGCAAAA AATCAAAGAC ATAAATGATC AGATGGATGA GTCTTTCAGA 
GAGTTGGATA TGGAATGTGC TCTTTTGGAT GGAGAACAGA AATCTGAAAC AACTGAACTT 
ATGAAGGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TAGCTGAACT GGAAAAGAAC 
ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG 
AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG 
AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCTGACC TGTTGGATGT TGAAAGCAAA 
CACTTTGAAG ACCTGGAGTT CCAGCAGCTT GAACATGAGA GCCGTCTAGA TGAAGAAAAG 
GAGAACTTGA CTCAACAGCT CCTGCGTGAA GTTGCTGAAT ATCAACGGAA CATCGTTTCT 
AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCCAATC ACATTGTTCA GCAGGCTCAG 
AGAGAGCAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGTT GCAAAGAGAA 
AAGGAGAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT 
CCCGTTAACC CCAATACTTT AAAAGAGGGC TATATCAGTG TAAATGAGAT TAATGAGCCG 
TGTGGCAATT CCACGAATCT ATCCCCTTCC ACTCAGTTTC CTGCTGATGC TGATGCTGTT 
GCCACTGAGC CTGCCACAGC TGTGCTGGCG AGCCAGCCAC AGAGTAAAGA GCACTTTAGA 
AGTCTGGAAG AAAGGAAAAA ACAGCATAAA GAAGGCCTCT ATCTGAGTGA TACTTTGCCT 
CGAAAGAAAA CCACATCTTC CATCTCCCCA CATTTCAGCA GTGCTACTAT GGGGAGAAGC 
ATCACCCCAA AGGCCCATCT GCCCCTAGGA CAGAGTAACA GCTGTGGAAG TGTGCTCCCT 
CCCTCACTGG CAGCCATGGC CAAAGACTCA GAATCTCGGA GGATGCTCAG AGGTTATAAT 
CACCAACAGA TGAGTGAAGG ACACAGGCAG AAATCTGAAT TTTATAACCG CACAGCATCT 
GAATCAAATG TCTACTTGAA TAGTTTCCAT TATCCAGATC ACAGCTACAA GGACCAGGCC 
TTTGATACTC TGAGCCTCGA TAGCTCTGAT AGCATGGAGA CCAGCATCTC TGCTTGCTCA 
CCAGACAACA TCTCTAGTGC CAGCACTTCA AATATTGCTA GAATAGAAGA AATGGAGAGA 
CTTTTGAAGC AGGCTCATGC AGAAAAGACG CGGCTGCTCG AATCCAGGGA ACGGGAAATG 
GAAGCCAAAA AACGAGCCCT GGAAGAAGAA AAACGACGCC GGGAAATCCT GGAAAAACGA 
TTACAGGAAG AAACTAGCCA GAGGCAGAAG TTAATAGAAA AGGAAGTAAA AATAAGGGAG 
AGACAAAGGG CACAGGCTCG TCCTTTGACA CGCTACCTGC CTGTCCGGAA GGAAGACTTT 
GATTTGCGGA GCCATGTAGA GACTGCTGGC CACAATATTG ACACCTGTTA CCATGTATCA 
ATCACAGAGA AGACCTGCCG AGGATTCCTC ATCAAAATGG GTGGGAAAAT TAAAACGTGG 
AAAAAACGTT GGTTTGTTTT TGATCGGAAC AAGCGAACAT TCTCTTATTA TGCAGACAAG 
CATGAAACTA AATTGAAAGG AGTAATATAC TTTCAAGCCA TTGAAGAAGT CTATTATGAT 
CACCTCAAGA ATGCTAATAA GAGTCCTAAT CCGTTACTCA CCTTTAGCGT CAAGACTCAT 
GACAGAATCT ATTATATGGT AGCCCCATCG CCAGAAGCCA TGCGGATCTG GATGGATGTT 
ATAGTTACGG GGGCAGAAGG TTACACTCAC TTCTTGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001127911.1
CDS	173..3934
Translation	MEEHSYIQKELDLQNGSLEEDSVVHSVENDSQNMMESLSPKKYSSSLRFKANGDYSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGSKQFSYDGTDKNIPMKPPTPLLNTTSSLSGYPLGRADFDHYTGRDSERALRLSEKPPYSKYSSRHKSHDNVYSLGGLEGRKASGSLLAMWNGSSLSDAGPPPISRSGAASMPSSPKQARKMSIQDSLALQPKLTRHKELASENINLRTRKYSSSSLSHMGAYSRSLPRLYRATENQLTPLSLPPRNSLGNSKRTKLGEKDLPHSVIDNDNYLNFSSLSSGALPYKTSASEGNPYVSSTLSVPASPRVARKMLLASTSSCASDDFDQASYVGTNPSHSLLAGESDRVFATRRNFSCGSVEFDEADLESLRQASGTPQPALRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRLSTGTTVEDVQKINKELEKLQLSDEESVFEEALMSPDTRYRCHRKDSLPDADLASCGSLSQSSASFFTPRSTRNDELLSDLTRTPPPPSSTFPKASSESSYLSILPKTPEGISEEQRSQELAAMEETRIVILNNLEELKQKIKDINDQMDESFRELDMECALLDGEQKSETTELMKEKEILDHLNRKIAELEKNIVGEKTKEKVKLDAEREKLERLQELYSEQKTQLDNCPESMREQLQQQLKRDADLLDVESKHFEDLEFQQLEHESRLDEEKENLTQQLLREVAEYQRNIVSRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVNPNTLKEGYISVNEINEPCGNSTNLSPSTQFPADADAVATEPATAVLASQPQSKEHFRSLEERKKQHKEGLYLSDTLPRKKTTSSISPHFSSATMGRSITPKAHLPLGQSNSCGSVLPPSLAAMAKDSESRRMLRGYNHQQMSEGHRQKSEFYNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRREILEKRLQEETSQRQKLIEKEVKIRERQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHETKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	NM_001134439.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134439.1

ORF Insert Sequence:

ATGGAAGAGC ATAGCTACAT ACAAAAGGAG CTAGATTTAC AAAATGGTAG CTTAGAGGAA 
GACTCTGTGG TGCATTCTGT TGAGAACGAT TCCCAAAACA TGATGGAGAG CCTCAGCCCA 
AAGAAATACT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTATTCTGG CTCCTATTTA 
ACCCTCTCAC AACCTGTGCC TGCAAAGAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA 
AGCAGCCCCT CCTTAGCCAA AATCCAGGGA AGCAAGCAGT TCTCTTATGA TGGAACTGAC 
AAAAATATTC CTATGAAACC TCCAACTCCT TTACTCAACA CTACATCCTC CCTCAGTGGA 
TATCCACTTG GAAGAGCAGA CTTTGATCAT TATACTGGCC GGGACAGTGA AAGGGCCTTG 
AGGCTCTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGCATAAATC GCATGACAAT 
GTCTACTCTC TTGGAGGGCT GGAAGGTCGG AAGGCATCTG GCTCGCTCCT GGCCATGTGG 
AATGGAAGTT CCCTGAGTGA TGCTGGCCCG CCTCCTATCA GCAGATCGGG AGCCGCAAGC 
ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATGAGCATTC AGGACAGCCT GGCGCTTCAA 
CCCAAGTTAA CTAGACACAA GGAGCTTGCA TCTGAAAACA TCAATTTGAG AACTAGGAAG 
TACTCCAGCA GCAGCCTGAG TCACATGGGA GCCTACAGCC GATCACTTCC CAGGTTGTAC 
AGAGCCACAG AGAACCAGCT GACACCTCTC AGCTTGCCTC CAAGAAACTC TCTGGGCAAT 
TCCAAACGAA CAAAACTTGG GGAAAAGGAT CTACCTCATA GCGTAATAGA CAATGACAAT 
TACCTTAATT TTTCTTCTTT GAGCTCAGGG GCTTTACCCT ATAAAACCTC TGCTTCTGAA 
GGCAATCCTT ATGTAAGTTC TACCCTCAGT GTCCCTGCCA GTCCACGAGT GGCTCGGAAG 
ATGCTTCTGG CCTCCACCTC CTCCTGTGCC TCTGATGACT TTGATCAGGC TTCATATGTG 
GGGACAAACC CGAGTCATTC ACTTCTTGCT GGAGAGTCAG ACAGAGTTTT TGCGACCAGG 
AGGAACTTCT CTTGTGGATC TGTGGAATTT GATGAGGCAG ATTTGGAAAG CCTCAGACAG 
GCCTCAGGAA CCCCCCAGCC TGCCCTTCGG GAACGGAAAA GCAGTATTAG CTCCATTTCA 
GGACGTGATG ACCTGATGGA TTATCACCGG CGGCAGAGGG AGGAAAGACT CAGGGAGCAG 
GAAATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGTCTCTG TGCTGAATAC 
ACAAAGCCTG ACAGTCGCTT ATCTACTGGG ACCACCGTGG AAGATGTGCA GAAAATCAAC 
AAGGAGCTTG AGAAGCTGCA GCTCTCTGAT GAGGAGTCTG TGTTTGAGGA AGCCCTCATG 
AGCCCTGACA CAAGATACAG GTGCCACCGG AAAGACTCCC TCCCTGATGC AGACTTGGCA 
AGCTGTGGGA GTCTCAGTCA GAGCAGTGCC AGCTTCTTTA CCCCCAGGAG CACCAGGAAT 
GATGAACTAC TCAGTGACCT CACCCGGACT CCTCCACCAC CATCCTCCAC CTTTCCGAAA 
GCTTCCAGCG AGTCCTCTTA TCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA 
GAACAGAGAT CTCAGGAGTT GGCTGCAATG GAAGAAACCC GGATAGTCAT TCTGAACAAC 
CTCGAGGAAC TTAAGCAAAA AATCAAAGAC ATAAATGATC AGATGGATGA GTCTTTCAGA 
GAGTTGGATA TGGAATGTGC TCTTTTGGAT GGAGAACAGA AATCTGAAAC AACTGAACTT 
ATGAAGGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TAGCTGAACT GGAAAAGAAC 
ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG 
AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG 
AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCTGACC TGTTGGATGT TGAAAGCAAA 
CACTTTGAAG ACCTGGAGTT CCAGCAGCTT GAACATGAGA GCCGTCTAGA TGAAGAAAAG 
GAGAACTTGA CTCAACAGCT CCTGCGTGAA GTTGCTGAAT ATCAACGGAA CATCGTTTCT 
AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCCAATC ACATTGTTCA GCAGGCTCAG 
AGAGAGCAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGTT GCAAAGAGAA 
AAGGAGAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT 
CCCGTTAACC CCAATACTTT AAAAGAGGGC TATATCAGTG TAAATGAGAT TAATGAGCCG 
TGTGGCAATT CCACGAATCT ATCCCCTTCC ACTCAGTTTC CTGCTGATGC TGATGCTGTT 
GCCACTGAGC CTGCCACAGC TGTGCTGGCG AGCCAGCCAC AGAGTAAAGA GCACTTTAGA 
AGTCTGGAAG AAAGGAAAAA ACAGCATAAA GAAGGCCTCT ATCTGAGTGA TACTTTGCCT 
CGAAAGAAAA CCACATCTTC CATCTCCCCA CATTTCAGCA GTGCTACTAT GGGGAGAAGC 
ATCACCCCAA AGGCCCATCT GCCCCTAGGA CAGAGTAACA GCTGTGGAAG TGTGCTCCCT 
CCCTCACTGG CAGCCATGGC CAAAGACTCA GAATCTCGGA GGATGCTCAG AGGTTATAAT 
CACCAACAGA TGAGTGAAGG ACACAGGCAG AAATCTGAAT TTTATAACCG CACAGCATCT 
GAATCAAATG TCTACTTGAA TAGTTTCCAT TATCCAGATC ACAGCTACAA GGACCAGGCC 
TTTGATACTC TGAGCCTCGA TAGCTCTGAT AGCATGGAGA CCAGCATCTC TGCTTGCTCA 
CCAGACAACA TCTCTAGTGC CAGCACTTCA AATATTGCTA GAATAGAAGA AATGGAGAGA 
CTTTTGAAGC AGGCTCATGC AGAAAAGACG CGGCTGCTCG AATCCAGGGA ACGGGAAATG 
GAAGCCAAAA AACGAGCCCT GGAAGAAGAA AAACGACGCC GGGAAATCCT GGAAAAACGA 
TTACAGGAAG AAACTAGCCA GAGGCAGAAG TTAATAGAAA AGGAAGTAAA AATAAGGGAG 
AGACAAAGGG CACAGGCTCG TCCTTTGACA CGCTACCTGC CTGTCCGGAA GGAAGACTTT 
GATTTGCGGA GCCATGTAGA GACTGCTGGC CACAATATTG ACACCTGTTA CCATGTATCA 
ATCACAGAGA AGACCTGCCG AGGATTCCTC ATCAAAATGG GTGGGAAAAT TAAAACGTGG 
AAAAAACGTT GGTTTGTTTT TGATCGGAAC AAGCGAACAT TCTCTTATTA TGCAGACAAG 
CATGAAACTA AATTGAAAGG AGTAATATAC TTTCAAGCCA TTGAAGAAGT CTATTATGAT 
CACCTCAAGA ATGCTAATAA GAGTCCTAAT CCGTTACTCA CCTTTAGCGT CAAGACTCAT 
GACAGAATCT ATTATATGGT AGCCCCATCG CCAGAAGCCA TGCGGATCTG GATGGATGTT 
ATAGTTACGG GGGCAGAAGG TTACACTCAC TTCTTGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( NM_001134438.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001127910.1 Homo sapiens pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26090
Clone ID Related Accession (Same CDS sequence)	NM_001134439.1 , NM_001134438.2 , NM_001134438.1 , NM_001134439.2
Accession Version	NM_001134438.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	pleckstrin homology-like domain family B member 2 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA438817.1, AK315050.1, AC060225.12 and AW264132.1. On May 17, 2019 this sequence version replaced NM_001134438.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Variants 1 and 2 both encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK315050.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000431670.7/ ENSP00000405405.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAAGAGC ATAGCTACAT ACAAAAGGAG CTAGATTTAC AAAATGGTAG CTTAGAGGAA 
GACTCTGTGG TGCATTCTGT TGAGAACGAT TCCCAAAACA TGATGGAGAG CCTCAGCCCA 
AAGAAATACT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTATTCTGG CTCCTATTTA 
ACCCTCTCAC AACCTGTGCC TGCAAAGAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA 
AGCAGCCCCT CCTTAGCCAA AATCCAGGGA AGCAAGCAGT TCTCTTATGA TGGAACTGAC 
AAAAATATTC CTATGAAACC TCCAACTCCT TTACTCAACA CTACATCCTC CCTCAGTGGA 
TATCCACTTG GAAGAGCAGA CTTTGATCAT TATACTGGCC GGGACAGTGA AAGGGCCTTG 
AGGCTCTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGCATAAATC GCATGACAAT 
GTCTACTCTC TTGGAGGGCT GGAAGGTCGG AAGGCATCTG GCTCGCTCCT GGCCATGTGG 
AATGGAAGTT CCCTGAGTGA TGCTGGCCCG CCTCCTATCA GCAGATCGGG AGCCGCAAGC 
ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATGAGCATTC AGGACAGCCT GGCGCTTCAA 
CCCAAGTTAA CTAGACACAA GGAGCTTGCA TCTGAAAACA TCAATTTGAG AACTAGGAAG 
TACTCCAGCA GCAGCCTGAG TCACATGGGA GCCTACAGCC GATCACTTCC CAGGTTGTAC 
AGAGCCACAG AGAACCAGCT GACACCTCTC AGCTTGCCTC CAAGAAACTC TCTGGGCAAT 
TCCAAACGAA CAAAACTTGG GGAAAAGGAT CTACCTCATA GCGTAATAGA CAATGACAAT 
TACCTTAATT TTTCTTCTTT GAGCTCAGGG GCTTTACCCT ATAAAACCTC TGCTTCTGAA 
GGCAATCCTT ATGTAAGTTC TACCCTCAGT GTCCCTGCCA GTCCACGAGT GGCTCGGAAG 
ATGCTTCTGG CCTCCACCTC CTCCTGTGCC TCTGATGACT TTGATCAGGC TTCATATGTG 
GGGACAAACC CGAGTCATTC ACTTCTTGCT GGAGAGTCAG ACAGAGTTTT TGCGACCAGG 
AGGAACTTCT CTTGTGGATC TGTGGAATTT GATGAGGCAG ATTTGGAAAG CCTCAGACAG 
GCCTCAGGAA CCCCCCAGCC TGCCCTTCGG GAACGGAAAA GCAGTATTAG CTCCATTTCA 
GGACGTGATG ACCTGATGGA TTATCACCGG CGGCAGAGGG AGGAAAGACT CAGGGAGCAG 
GAAATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGTCTCTG TGCTGAATAC 
ACAAAGCCTG ACAGTCGCTT ATCTACTGGG ACCACCGTGG AAGATGTGCA GAAAATCAAC 
AAGGAGCTTG AGAAGCTGCA GCTCTCTGAT GAGGAGTCTG TGTTTGAGGA AGCCCTCATG 
AGCCCTGACA CAAGATACAG GTGCCACCGG AAAGACTCCC TCCCTGATGC AGACTTGGCA 
AGCTGTGGGA GTCTCAGTCA GAGCAGTGCC AGCTTCTTTA CCCCCAGGAG CACCAGGAAT 
GATGAACTAC TCAGTGACCT CACCCGGACT CCTCCACCAC CATCCTCCAC CTTTCCGAAA 
GCTTCCAGCG AGTCCTCTTA TCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA 
GAACAGAGAT CTCAGGAGTT GGCTGCAATG GAAGAAACCC GGATAGTCAT TCTGAACAAC 
CTCGAGGAAC TTAAGCAAAA AATCAAAGAC ATAAATGATC AGATGGATGA GTCTTTCAGA 
GAGTTGGATA TGGAATGTGC TCTTTTGGAT GGAGAACAGA AATCTGAAAC AACTGAACTT 
ATGAAGGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TAGCTGAACT GGAAAAGAAC 
ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG 
AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG 
AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCTGACC TGTTGGATGT TGAAAGCAAA 
CACTTTGAAG ACCTGGAGTT CCAGCAGCTT GAACATGAGA GCCGTCTAGA TGAAGAAAAG 
GAGAACTTGA CTCAACAGCT CCTGCGTGAA GTTGCTGAAT ATCAACGGAA CATCGTTTCT 
AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCCAATC ACATTGTTCA GCAGGCTCAG 
AGAGAGCAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGTT GCAAAGAGAA 
AAGGAGAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT 
CCCGTTAACC CCAATACTTT AAAAGAGGGC TATATCAGTG TAAATGAGAT TAATGAGCCG 
TGTGGCAATT CCACGAATCT ATCCCCTTCC ACTCAGTTTC CTGCTGATGC TGATGCTGTT 
GCCACTGAGC CTGCCACAGC TGTGCTGGCG AGCCAGCCAC AGAGTAAAGA GCACTTTAGA 
AGTCTGGAAG AAAGGAAAAA ACAGCATAAA GAAGGCCTCT ATCTGAGTGA TACTTTGCCT 
CGAAAGAAAA CCACATCTTC CATCTCCCCA CATTTCAGCA GTGCTACTAT GGGGAGAAGC 
ATCACCCCAA AGGCCCATCT GCCCCTAGGA CAGAGTAACA GCTGTGGAAG TGTGCTCCCT 
CCCTCACTGG CAGCCATGGC CAAAGACTCA GAATCTCGGA GGATGCTCAG AGGTTATAAT 
CACCAACAGA TGAGTGAAGG ACACAGGCAG AAATCTGAAT TTTATAACCG CACAGCATCT 
GAATCAAATG TCTACTTGAA TAGTTTCCAT TATCCAGATC ACAGCTACAA GGACCAGGCC 
TTTGATACTC TGAGCCTCGA TAGCTCTGAT AGCATGGAGA CCAGCATCTC TGCTTGCTCA 
CCAGACAACA TCTCTAGTGC CAGCACTTCA AATATTGCTA GAATAGAAGA AATGGAGAGA 
CTTTTGAAGC AGGCTCATGC AGAAAAGACG CGGCTGCTCG AATCCAGGGA ACGGGAAATG 
GAAGCCAAAA AACGAGCCCT GGAAGAAGAA AAACGACGCC GGGAAATCCT GGAAAAACGA 
TTACAGGAAG AAACTAGCCA GAGGCAGAAG TTAATAGAAA AGGAAGTAAA AATAAGGGAG 
AGACAAAGGG CACAGGCTCG TCCTTTGACA CGCTACCTGC CTGTCCGGAA GGAAGACTTT 
GATTTGCGGA GCCATGTAGA GACTGCTGGC CACAATATTG ACACCTGTTA CCATGTATCA 
ATCACAGAGA AGACCTGCCG AGGATTCCTC ATCAAAATGG GTGGGAAAAT TAAAACGTGG 
AAAAAACGTT GGTTTGTTTT TGATCGGAAC AAGCGAACAT TCTCTTATTA TGCAGACAAG 
CATGAAACTA AATTGAAAGG AGTAATATAC TTTCAAGCCA TTGAAGAAGT CTATTATGAT 
CACCTCAAGA ATGCTAATAA GAGTCCTAAT CCGTTACTCA CCTTTAGCGT CAAGACTCAT 
GACAGAATCT ATTATATGGT AGCCCCATCG CCAGAAGCCA TGCGGATCTG GATGGATGTT 
ATAGTTACGG GGGCAGAAGG TTACACTCAC TTCTTGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001127910.1
CDS	327..4088
Translation	MEEHSYIQKELDLQNGSLEEDSVVHSVENDSQNMMESLSPKKYSSSLRFKANGDYSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGSKQFSYDGTDKNIPMKPPTPLLNTTSSLSGYPLGRADFDHYTGRDSERALRLSEKPPYSKYSSRHKSHDNVYSLGGLEGRKASGSLLAMWNGSSLSDAGPPPISRSGAASMPSSPKQARKMSIQDSLALQPKLTRHKELASENINLRTRKYSSSSLSHMGAYSRSLPRLYRATENQLTPLSLPPRNSLGNSKRTKLGEKDLPHSVIDNDNYLNFSSLSSGALPYKTSASEGNPYVSSTLSVPASPRVARKMLLASTSSCASDDFDQASYVGTNPSHSLLAGESDRVFATRRNFSCGSVEFDEADLESLRQASGTPQPALRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRLSTGTTVEDVQKINKELEKLQLSDEESVFEEALMSPDTRYRCHRKDSLPDADLASCGSLSQSSASFFTPRSTRNDELLSDLTRTPPPPSSTFPKASSESSYLSILPKTPEGISEEQRSQELAAMEETRIVILNNLEELKQKIKDINDQMDESFRELDMECALLDGEQKSETTELMKEKEILDHLNRKIAELEKNIVGEKTKEKVKLDAEREKLERLQELYSEQKTQLDNCPESMREQLQQQLKRDADLLDVESKHFEDLEFQQLEHESRLDEEKENLTQQLLREVAEYQRNIVSRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVNPNTLKEGYISVNEINEPCGNSTNLSPSTQFPADADAVATEPATAVLASQPQSKEHFRSLEERKKQHKEGLYLSDTLPRKKTTSSISPHFSSATMGRSITPKAHLPLGQSNSCGSVLPPSLAAMAKDSESRRMLRGYNHQQMSEGHRQKSEFYNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRREILEKRLQEETSQRQKLIEKEVKIRERQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHETKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	NM_001134438.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134438.2

ORF Insert Sequence:

ATGGAAGAGC ATAGCTACAT ACAAAAGGAG CTAGATTTAC AAAATGGTAG CTTAGAGGAA 
GACTCTGTGG TGCATTCTGT TGAGAACGAT TCCCAAAACA TGATGGAGAG CCTCAGCCCA 
AAGAAATACT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTATTCTGG CTCCTATTTA 
ACCCTCTCAC AACCTGTGCC TGCAAAGAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA 
AGCAGCCCCT CCTTAGCCAA AATCCAGGGA AGCAAGCAGT TCTCTTATGA TGGAACTGAC 
AAAAATATTC CTATGAAACC TCCAACTCCT TTACTCAACA CTACATCCTC CCTCAGTGGA 
TATCCACTTG GAAGAGCAGA CTTTGATCAT TATACTGGCC GGGACAGTGA AAGGGCCTTG 
AGGCTCTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGCATAAATC GCATGACAAT 
GTCTACTCTC TTGGAGGGCT GGAAGGTCGG AAGGCATCTG GCTCGCTCCT GGCCATGTGG 
AATGGAAGTT CCCTGAGTGA TGCTGGCCCG CCTCCTATCA GCAGATCGGG AGCCGCAAGC 
ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATGAGCATTC AGGACAGCCT GGCGCTTCAA 
CCCAAGTTAA CTAGACACAA GGAGCTTGCA TCTGAAAACA TCAATTTGAG AACTAGGAAG 
TACTCCAGCA GCAGCCTGAG TCACATGGGA GCCTACAGCC GATCACTTCC CAGGTTGTAC 
AGAGCCACAG AGAACCAGCT GACACCTCTC AGCTTGCCTC CAAGAAACTC TCTGGGCAAT 
TCCAAACGAA CAAAACTTGG GGAAAAGGAT CTACCTCATA GCGTAATAGA CAATGACAAT 
TACCTTAATT TTTCTTCTTT GAGCTCAGGG GCTTTACCCT ATAAAACCTC TGCTTCTGAA 
GGCAATCCTT ATGTAAGTTC TACCCTCAGT GTCCCTGCCA GTCCACGAGT GGCTCGGAAG 
ATGCTTCTGG CCTCCACCTC CTCCTGTGCC TCTGATGACT TTGATCAGGC TTCATATGTG 
GGGACAAACC CGAGTCATTC ACTTCTTGCT GGAGAGTCAG ACAGAGTTTT TGCGACCAGG 
AGGAACTTCT CTTGTGGATC TGTGGAATTT GATGAGGCAG ATTTGGAAAG CCTCAGACAG 
GCCTCAGGAA CCCCCCAGCC TGCCCTTCGG GAACGGAAAA GCAGTATTAG CTCCATTTCA 
GGACGTGATG ACCTGATGGA TTATCACCGG CGGCAGAGGG AGGAAAGACT CAGGGAGCAG 
GAAATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGTCTCTG TGCTGAATAC 
ACAAAGCCTG ACAGTCGCTT ATCTACTGGG ACCACCGTGG AAGATGTGCA GAAAATCAAC 
AAGGAGCTTG AGAAGCTGCA GCTCTCTGAT GAGGAGTCTG TGTTTGAGGA AGCCCTCATG 
AGCCCTGACA CAAGATACAG GTGCCACCGG AAAGACTCCC TCCCTGATGC AGACTTGGCA 
AGCTGTGGGA GTCTCAGTCA GAGCAGTGCC AGCTTCTTTA CCCCCAGGAG CACCAGGAAT 
GATGAACTAC TCAGTGACCT CACCCGGACT CCTCCACCAC CATCCTCCAC CTTTCCGAAA 
GCTTCCAGCG AGTCCTCTTA TCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA 
GAACAGAGAT CTCAGGAGTT GGCTGCAATG GAAGAAACCC GGATAGTCAT TCTGAACAAC 
CTCGAGGAAC TTAAGCAAAA AATCAAAGAC ATAAATGATC AGATGGATGA GTCTTTCAGA 
GAGTTGGATA TGGAATGTGC TCTTTTGGAT GGAGAACAGA AATCTGAAAC AACTGAACTT 
ATGAAGGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TAGCTGAACT GGAAAAGAAC 
ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG 
AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG 
AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCTGACC TGTTGGATGT TGAAAGCAAA 
CACTTTGAAG ACCTGGAGTT CCAGCAGCTT GAACATGAGA GCCGTCTAGA TGAAGAAAAG 
GAGAACTTGA CTCAACAGCT CCTGCGTGAA GTTGCTGAAT ATCAACGGAA CATCGTTTCT 
AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCCAATC ACATTGTTCA GCAGGCTCAG 
AGAGAGCAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGTT GCAAAGAGAA 
AAGGAGAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT 
CCCGTTAACC CCAATACTTT AAAAGAGGGC TATATCAGTG TAAATGAGAT TAATGAGCCG 
TGTGGCAATT CCACGAATCT ATCCCCTTCC ACTCAGTTTC CTGCTGATGC TGATGCTGTT 
GCCACTGAGC CTGCCACAGC TGTGCTGGCG AGCCAGCCAC AGAGTAAAGA GCACTTTAGA 
AGTCTGGAAG AAAGGAAAAA ACAGCATAAA GAAGGCCTCT ATCTGAGTGA TACTTTGCCT 
CGAAAGAAAA CCACATCTTC CATCTCCCCA CATTTCAGCA GTGCTACTAT GGGGAGAAGC 
ATCACCCCAA AGGCCCATCT GCCCCTAGGA CAGAGTAACA GCTGTGGAAG TGTGCTCCCT 
CCCTCACTGG CAGCCATGGC CAAAGACTCA GAATCTCGGA GGATGCTCAG AGGTTATAAT 
CACCAACAGA TGAGTGAAGG ACACAGGCAG AAATCTGAAT TTTATAACCG CACAGCATCT 
GAATCAAATG TCTACTTGAA TAGTTTCCAT TATCCAGATC ACAGCTACAA GGACCAGGCC 
TTTGATACTC TGAGCCTCGA TAGCTCTGAT AGCATGGAGA CCAGCATCTC TGCTTGCTCA 
CCAGACAACA TCTCTAGTGC CAGCACTTCA AATATTGCTA GAATAGAAGA AATGGAGAGA 
CTTTTGAAGC AGGCTCATGC AGAAAAGACG CGGCTGCTCG AATCCAGGGA ACGGGAAATG 
GAAGCCAAAA AACGAGCCCT GGAAGAAGAA AAACGACGCC GGGAAATCCT GGAAAAACGA 
TTACAGGAAG AAACTAGCCA GAGGCAGAAG TTAATAGAAA AGGAAGTAAA AATAAGGGAG 
AGACAAAGGG CACAGGCTCG TCCTTTGACA CGCTACCTGC CTGTCCGGAA GGAAGACTTT 
GATTTGCGGA GCCATGTAGA GACTGCTGGC CACAATATTG ACACCTGTTA CCATGTATCA 
ATCACAGAGA AGACCTGCCG AGGATTCCTC ATCAAAATGG GTGGGAAAAT TAAAACGTGG 
AAAAAACGTT GGTTTGTTTT TGATCGGAAC AAGCGAACAT TCTCTTATTA TGCAGACAAG 
CATGAAACTA AATTGAAAGG AGTAATATAC TTTCAAGCCA TTGAAGAAGT CTATTATGAT 
CACCTCAAGA ATGCTAATAA GAGTCCTAAT CCGTTACTCA CCTTTAGCGT CAAGACTCAT 
GACAGAATCT ATTATATGGT AGCCCCATCG CCAGAAGCCA TGCGGATCTG GATGGATGTT 
ATAGTTACGG GGGCAGAAGG TTACACTCAC TTCTTGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( NM_001134438.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001127910.1 Homo sapiens pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant 1, mRNA.

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CloneID	OHu26090
Clone ID Related Accession (Same CDS sequence)	NM_001134439.1 , NM_001134438.2 , NM_001134438.1 , NM_001134439.2
Accession Version	NM_001134438.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Homo sapiens(human)
Product	pleckstrin homology-like domain family B member 2 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA438817.1, AK315050.1, AC060225.12 and AW264132.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Variants 1 and 2 both encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK315050.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGAGC ATAGCTACAT ACAAAAGGAG CTAGATTTAC AAAATGGTAG CTTAGAGGAA 
GACTCTGTGG TGCATTCTGT TGAGAACGAT TCCCAAAACA TGATGGAGAG CCTCAGCCCA 
AAGAAATACT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTATTCTGG CTCCTATTTA 
ACCCTCTCAC AACCTGTGCC TGCAAAGAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA 
AGCAGCCCCT CCTTAGCCAA AATCCAGGGA AGCAAGCAGT TCTCTTATGA TGGAACTGAC 
AAAAATATTC CTATGAAACC TCCAACTCCT TTACTCAACA CTACATCCTC CCTCAGTGGA 
TATCCACTTG GAAGAGCAGA CTTTGATCAT TATACTGGCC GGGACAGTGA AAGGGCCTTG 
AGGCTCTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGCATAAATC GCATGACAAT 
GTCTACTCTC TTGGAGGGCT GGAAGGTCGG AAGGCATCTG GCTCGCTCCT GGCCATGTGG 
AATGGAAGTT CCCTGAGTGA TGCTGGCCCG CCTCCTATCA GCAGATCGGG AGCCGCAAGC 
ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATGAGCATTC AGGACAGCCT GGCGCTTCAA 
CCCAAGTTAA CTAGACACAA GGAGCTTGCA TCTGAAAACA TCAATTTGAG AACTAGGAAG 
TACTCCAGCA GCAGCCTGAG TCACATGGGA GCCTACAGCC GATCACTTCC CAGGTTGTAC 
AGAGCCACAG AGAACCAGCT GACACCTCTC AGCTTGCCTC CAAGAAACTC TCTGGGCAAT 
TCCAAACGAA CAAAACTTGG GGAAAAGGAT CTACCTCATA GCGTAATAGA CAATGACAAT 
TACCTTAATT TTTCTTCTTT GAGCTCAGGG GCTTTACCCT ATAAAACCTC TGCTTCTGAA 
GGCAATCCTT ATGTAAGTTC TACCCTCAGT GTCCCTGCCA GTCCACGAGT GGCTCGGAAG 
ATGCTTCTGG CCTCCACCTC CTCCTGTGCC TCTGATGACT TTGATCAGGC TTCATATGTG 
GGGACAAACC CGAGTCATTC ACTTCTTGCT GGAGAGTCAG ACAGAGTTTT TGCGACCAGG 
AGGAACTTCT CTTGTGGATC TGTGGAATTT GATGAGGCAG ATTTGGAAAG CCTCAGACAG 
GCCTCAGGAA CCCCCCAGCC TGCCCTTCGG GAACGGAAAA GCAGTATTAG CTCCATTTCA 
GGACGTGATG ACCTGATGGA TTATCACCGG CGGCAGAGGG AGGAAAGACT CAGGGAGCAG 
GAAATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGTCTCTG TGCTGAATAC 
ACAAAGCCTG ACAGTCGCTT ATCTACTGGG ACCACCGTGG AAGATGTGCA GAAAATCAAC 
AAGGAGCTTG AGAAGCTGCA GCTCTCTGAT GAGGAGTCTG TGTTTGAGGA AGCCCTCATG 
AGCCCTGACA CAAGATACAG GTGCCACCGG AAAGACTCCC TCCCTGATGC AGACTTGGCA 
AGCTGTGGGA GTCTCAGTCA GAGCAGTGCC AGCTTCTTTA CCCCCAGGAG CACCAGGAAT 
GATGAACTAC TCAGTGACCT CACCCGGACT CCTCCACCAC CATCCTCCAC CTTTCCGAAA 
GCTTCCAGCG AGTCCTCTTA TCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA 
GAACAGAGAT CTCAGGAGTT GGCTGCAATG GAAGAAACCC GGATAGTCAT TCTGAACAAC 
CTCGAGGAAC TTAAGCAAAA AATCAAAGAC ATAAATGATC AGATGGATGA GTCTTTCAGA 
GAGTTGGATA TGGAATGTGC TCTTTTGGAT GGAGAACAGA AATCTGAAAC AACTGAACTT 
ATGAAGGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TAGCTGAACT GGAAAAGAAC 
ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG 
AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG 
AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCTGACC TGTTGGATGT TGAAAGCAAA 
CACTTTGAAG ACCTGGAGTT CCAGCAGCTT GAACATGAGA GCCGTCTAGA TGAAGAAAAG 
GAGAACTTGA CTCAACAGCT CCTGCGTGAA GTTGCTGAAT ATCAACGGAA CATCGTTTCT 
AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCCAATC ACATTGTTCA GCAGGCTCAG 
AGAGAGCAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGTT GCAAAGAGAA 
AAGGAGAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT 
CCCGTTAACC CCAATACTTT AAAAGAGGGC TATATCAGTG TAAATGAGAT TAATGAGCCG 
TGTGGCAATT CCACGAATCT ATCCCCTTCC ACTCAGTTTC CTGCTGATGC TGATGCTGTT 
GCCACTGAGC CTGCCACAGC TGTGCTGGCG AGCCAGCCAC AGAGTAAAGA GCACTTTAGA 
AGTCTGGAAG AAAGGAAAAA ACAGCATAAA GAAGGCCTCT ATCTGAGTGA TACTTTGCCT 
CGAAAGAAAA CCACATCTTC CATCTCCCCA CATTTCAGCA GTGCTACTAT GGGGAGAAGC 
ATCACCCCAA AGGCCCATCT GCCCCTAGGA CAGAGTAACA GCTGTGGAAG TGTGCTCCCT 
CCCTCACTGG CAGCCATGGC CAAAGACTCA GAATCTCGGA GGATGCTCAG AGGTTATAAT 
CACCAACAGA TGAGTGAAGG ACACAGGCAG AAATCTGAAT TTTATAACCG CACAGCATCT 
GAATCAAATG TCTACTTGAA TAGTTTCCAT TATCCAGATC ACAGCTACAA GGACCAGGCC 
TTTGATACTC TGAGCCTCGA TAGCTCTGAT AGCATGGAGA CCAGCATCTC TGCTTGCTCA 
CCAGACAACA TCTCTAGTGC CAGCACTTCA AATATTGCTA GAATAGAAGA AATGGAGAGA 
CTTTTGAAGC AGGCTCATGC AGAAAAGACG CGGCTGCTCG AATCCAGGGA ACGGGAAATG 
GAAGCCAAAA AACGAGCCCT GGAAGAAGAA AAACGACGCC GGGAAATCCT GGAAAAACGA 
TTACAGGAAG AAACTAGCCA GAGGCAGAAG TTAATAGAAA AGGAAGTAAA AATAAGGGAG 
AGACAAAGGG CACAGGCTCG TCCTTTGACA CGCTACCTGC CTGTCCGGAA GGAAGACTTT 
GATTTGCGGA GCCATGTAGA GACTGCTGGC CACAATATTG ACACCTGTTA CCATGTATCA 
ATCACAGAGA AGACCTGCCG AGGATTCCTC ATCAAAATGG GTGGGAAAAT TAAAACGTGG 
AAAAAACGTT GGTTTGTTTT TGATCGGAAC AAGCGAACAT TCTCTTATTA TGCAGACAAG 
CATGAAACTA AATTGAAAGG AGTAATATAC TTTCAAGCCA TTGAAGAAGT CTATTATGAT 
CACCTCAAGA ATGCTAATAA GAGTCCTAAT CCGTTACTCA CCTTTAGCGT CAAGACTCAT 
GACAGAATCT ATTATATGGT AGCCCCATCG CCAGAAGCCA TGCGGATCTG GATGGATGTT 
ATAGTTACGG GGGCAGAAGG TTACACTCAC TTCTTGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001127910.1
CDS	412..4173
Translation	MEEHSYIQKELDLQNGSLEEDSVVHSVENDSQNMMESLSPKKYSSSLRFKANGDYSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGSKQFSYDGTDKNIPMKPPTPLLNTTSSLSGYPLGRADFDHYTGRDSERALRLSEKPPYSKYSSRHKSHDNVYSLGGLEGRKASGSLLAMWNGSSLSDAGPPPISRSGAASMPSSPKQARKMSIQDSLALQPKLTRHKELASENINLRTRKYSSSSLSHMGAYSRSLPRLYRATENQLTPLSLPPRNSLGNSKRTKLGEKDLPHSVIDNDNYLNFSSLSSGALPYKTSASEGNPYVSSTLSVPASPRVARKMLLASTSSCASDDFDQASYVGTNPSHSLLAGESDRVFATRRNFSCGSVEFDEADLESLRQASGTPQPALRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRLSTGTTVEDVQKINKELEKLQLSDEESVFEEALMSPDTRYRCHRKDSLPDADLASCGSLSQSSASFFTPRSTRNDELLSDLTRTPPPPSSTFPKASSESSYLSILPKTPEGISEEQRSQELAAMEETRIVILNNLEELKQKIKDINDQMDESFRELDMECALLDGEQKSETTELMKEKEILDHLNRKIAELEKNIVGEKTKEKVKLDAEREKLERLQELYSEQKTQLDNCPESMREQLQQQLKRDADLLDVESKHFEDLEFQQLEHESRLDEEKENLTQQLLREVAEYQRNIVSRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVNPNTLKEGYISVNEINEPCGNSTNLSPSTQFPADADAVATEPATAVLASQPQSKEHFRSLEERKKQHKEGLYLSDTLPRKKTTSSISPHFSSATMGRSITPKAHLPLGQSNSCGSVLPPSLAAMAKDSESRRMLRGYNHQQMSEGHRQKSEFYNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRREILEKRLQEETSQRQKLIEKEVKIRERQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHETKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	NM_001134438.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134438.1

ORF Insert Sequence:

ATGGAAGAGC ATAGCTACAT ACAAAAGGAG CTAGATTTAC AAAATGGTAG CTTAGAGGAA 
GACTCTGTGG TGCATTCTGT TGAGAACGAT TCCCAAAACA TGATGGAGAG CCTCAGCCCA 
AAGAAATACT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTATTCTGG CTCCTATTTA 
ACCCTCTCAC AACCTGTGCC TGCAAAGAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA 
AGCAGCCCCT CCTTAGCCAA AATCCAGGGA AGCAAGCAGT TCTCTTATGA TGGAACTGAC 
AAAAATATTC CTATGAAACC TCCAACTCCT TTACTCAACA CTACATCCTC CCTCAGTGGA 
TATCCACTTG GAAGAGCAGA CTTTGATCAT TATACTGGCC GGGACAGTGA AAGGGCCTTG 
AGGCTCTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGCATAAATC GCATGACAAT 
GTCTACTCTC TTGGAGGGCT GGAAGGTCGG AAGGCATCTG GCTCGCTCCT GGCCATGTGG 
AATGGAAGTT CCCTGAGTGA TGCTGGCCCG CCTCCTATCA GCAGATCGGG AGCCGCAAGC 
ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATGAGCATTC AGGACAGCCT GGCGCTTCAA 
CCCAAGTTAA CTAGACACAA GGAGCTTGCA TCTGAAAACA TCAATTTGAG AACTAGGAAG 
TACTCCAGCA GCAGCCTGAG TCACATGGGA GCCTACAGCC GATCACTTCC CAGGTTGTAC 
AGAGCCACAG AGAACCAGCT GACACCTCTC AGCTTGCCTC CAAGAAACTC TCTGGGCAAT 
TCCAAACGAA CAAAACTTGG GGAAAAGGAT CTACCTCATA GCGTAATAGA CAATGACAAT 
TACCTTAATT TTTCTTCTTT GAGCTCAGGG GCTTTACCCT ATAAAACCTC TGCTTCTGAA 
GGCAATCCTT ATGTAAGTTC TACCCTCAGT GTCCCTGCCA GTCCACGAGT GGCTCGGAAG 
ATGCTTCTGG CCTCCACCTC CTCCTGTGCC TCTGATGACT TTGATCAGGC TTCATATGTG 
GGGACAAACC CGAGTCATTC ACTTCTTGCT GGAGAGTCAG ACAGAGTTTT TGCGACCAGG 
AGGAACTTCT CTTGTGGATC TGTGGAATTT GATGAGGCAG ATTTGGAAAG CCTCAGACAG 
GCCTCAGGAA CCCCCCAGCC TGCCCTTCGG GAACGGAAAA GCAGTATTAG CTCCATTTCA 
GGACGTGATG ACCTGATGGA TTATCACCGG CGGCAGAGGG AGGAAAGACT CAGGGAGCAG 
GAAATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGTCTCTG TGCTGAATAC 
ACAAAGCCTG ACAGTCGCTT ATCTACTGGG ACCACCGTGG AAGATGTGCA GAAAATCAAC 
AAGGAGCTTG AGAAGCTGCA GCTCTCTGAT GAGGAGTCTG TGTTTGAGGA AGCCCTCATG 
AGCCCTGACA CAAGATACAG GTGCCACCGG AAAGACTCCC TCCCTGATGC AGACTTGGCA 
AGCTGTGGGA GTCTCAGTCA GAGCAGTGCC AGCTTCTTTA CCCCCAGGAG CACCAGGAAT 
GATGAACTAC TCAGTGACCT CACCCGGACT CCTCCACCAC CATCCTCCAC CTTTCCGAAA 
GCTTCCAGCG AGTCCTCTTA TCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA 
GAACAGAGAT CTCAGGAGTT GGCTGCAATG GAAGAAACCC GGATAGTCAT TCTGAACAAC 
CTCGAGGAAC TTAAGCAAAA AATCAAAGAC ATAAATGATC AGATGGATGA GTCTTTCAGA 
GAGTTGGATA TGGAATGTGC TCTTTTGGAT GGAGAACAGA AATCTGAAAC AACTGAACTT 
ATGAAGGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TAGCTGAACT GGAAAAGAAC 
ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG 
AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG 
AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCTGACC TGTTGGATGT TGAAAGCAAA 
CACTTTGAAG ACCTGGAGTT CCAGCAGCTT GAACATGAGA GCCGTCTAGA TGAAGAAAAG 
GAGAACTTGA CTCAACAGCT CCTGCGTGAA GTTGCTGAAT ATCAACGGAA CATCGTTTCT 
AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCCAATC ACATTGTTCA GCAGGCTCAG 
AGAGAGCAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGTT GCAAAGAGAA 
AAGGAGAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT 
CCCGTTAACC CCAATACTTT AAAAGAGGGC TATATCAGTG TAAATGAGAT TAATGAGCCG 
TGTGGCAATT CCACGAATCT ATCCCCTTCC ACTCAGTTTC CTGCTGATGC TGATGCTGTT 
GCCACTGAGC CTGCCACAGC TGTGCTGGCG AGCCAGCCAC AGAGTAAAGA GCACTTTAGA 
AGTCTGGAAG AAAGGAAAAA ACAGCATAAA GAAGGCCTCT ATCTGAGTGA TACTTTGCCT 
CGAAAGAAAA CCACATCTTC CATCTCCCCA CATTTCAGCA GTGCTACTAT GGGGAGAAGC 
ATCACCCCAA AGGCCCATCT GCCCCTAGGA CAGAGTAACA GCTGTGGAAG TGTGCTCCCT 
CCCTCACTGG CAGCCATGGC CAAAGACTCA GAATCTCGGA GGATGCTCAG AGGTTATAAT 
CACCAACAGA TGAGTGAAGG ACACAGGCAG AAATCTGAAT TTTATAACCG CACAGCATCT 
GAATCAAATG TCTACTTGAA TAGTTTCCAT TATCCAGATC ACAGCTACAA GGACCAGGCC 
TTTGATACTC TGAGCCTCGA TAGCTCTGAT AGCATGGAGA CCAGCATCTC TGCTTGCTCA 
CCAGACAACA TCTCTAGTGC CAGCACTTCA AATATTGCTA GAATAGAAGA AATGGAGAGA 
CTTTTGAAGC AGGCTCATGC AGAAAAGACG CGGCTGCTCG AATCCAGGGA ACGGGAAATG 
GAAGCCAAAA AACGAGCCCT GGAAGAAGAA AAACGACGCC GGGAAATCCT GGAAAAACGA 
TTACAGGAAG AAACTAGCCA GAGGCAGAAG TTAATAGAAA AGGAAGTAAA AATAAGGGAG 
AGACAAAGGG CACAGGCTCG TCCTTTGACA CGCTACCTGC CTGTCCGGAA GGAAGACTTT 
GATTTGCGGA GCCATGTAGA GACTGCTGGC CACAATATTG ACACCTGTTA CCATGTATCA 
ATCACAGAGA AGACCTGCCG AGGATTCCTC ATCAAAATGG GTGGGAAAAT TAAAACGTGG 
AAAAAACGTT GGTTTGTTTT TGATCGGAAC AAGCGAACAT TCTCTTATTA TGCAGACAAG 
CATGAAACTA AATTGAAAGG AGTAATATAC TTTCAAGCCA TTGAAGAAGT CTATTATGAT 
CACCTCAAGA ATGCTAATAA GAGTCCTAAT CCGTTACTCA CCTTTAGCGT CAAGACTCAT 
GACAGAATCT ATTATATGGT AGCCCCATCG CCAGAAGCCA TGCGGATCTG GATGGATGTT 
ATAGTTACGG GGGCAGAAGG TTACACTCAC TTCTTGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( NM_001134439.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001127911.1 Homo sapiens pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant 2, mRNA.

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CloneID	OHu26090
Clone ID Related Accession (Same CDS sequence)	NM_001134439.1 , NM_001134438.2 , NM_001134438.1 , NM_001134439.2
Accession Version	NM_001134439.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	pleckstrin homology-like domain family B member 2 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA272187.1, AB209903.1, AC060225.12 and AW264132.1. On May 31, 2019 this sequence version replaced NM_001134439.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209903.1, SRR1803616.36787.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGAGC ATAGCTACAT ACAAAAGGAG CTAGATTTAC AAAATGGTAG CTTAGAGGAA 
GACTCTGTGG TGCATTCTGT TGAGAACGAT TCCCAAAACA TGATGGAGAG CCTCAGCCCA 
AAGAAATACT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTATTCTGG CTCCTATTTA 
ACCCTCTCAC AACCTGTGCC TGCAAAGAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA 
AGCAGCCCCT CCTTAGCCAA AATCCAGGGA AGCAAGCAGT TCTCTTATGA TGGAACTGAC 
AAAAATATTC CTATGAAACC TCCAACTCCT TTACTCAACA CTACATCCTC CCTCAGTGGA 
TATCCACTTG GAAGAGCAGA CTTTGATCAT TATACTGGCC GGGACAGTGA AAGGGCCTTG 
AGGCTCTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGCATAAATC GCATGACAAT 
GTCTACTCTC TTGGAGGGCT GGAAGGTCGG AAGGCATCTG GCTCGCTCCT GGCCATGTGG 
AATGGAAGTT CCCTGAGTGA TGCTGGCCCG CCTCCTATCA GCAGATCGGG AGCCGCAAGC 
ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATGAGCATTC AGGACAGCCT GGCGCTTCAA 
CCCAAGTTAA CTAGACACAA GGAGCTTGCA TCTGAAAACA TCAATTTGAG AACTAGGAAG 
TACTCCAGCA GCAGCCTGAG TCACATGGGA GCCTACAGCC GATCACTTCC CAGGTTGTAC 
AGAGCCACAG AGAACCAGCT GACACCTCTC AGCTTGCCTC CAAGAAACTC TCTGGGCAAT 
TCCAAACGAA CAAAACTTGG GGAAAAGGAT CTACCTCATA GCGTAATAGA CAATGACAAT 
TACCTTAATT TTTCTTCTTT GAGCTCAGGG GCTTTACCCT ATAAAACCTC TGCTTCTGAA 
GGCAATCCTT ATGTAAGTTC TACCCTCAGT GTCCCTGCCA GTCCACGAGT GGCTCGGAAG 
ATGCTTCTGG CCTCCACCTC CTCCTGTGCC TCTGATGACT TTGATCAGGC TTCATATGTG 
GGGACAAACC CGAGTCATTC ACTTCTTGCT GGAGAGTCAG ACAGAGTTTT TGCGACCAGG 
AGGAACTTCT CTTGTGGATC TGTGGAATTT GATGAGGCAG ATTTGGAAAG CCTCAGACAG 
GCCTCAGGAA CCCCCCAGCC TGCCCTTCGG GAACGGAAAA GCAGTATTAG CTCCATTTCA 
GGACGTGATG ACCTGATGGA TTATCACCGG CGGCAGAGGG AGGAAAGACT CAGGGAGCAG 
GAAATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGTCTCTG TGCTGAATAC 
ACAAAGCCTG ACAGTCGCTT ATCTACTGGG ACCACCGTGG AAGATGTGCA GAAAATCAAC 
AAGGAGCTTG AGAAGCTGCA GCTCTCTGAT GAGGAGTCTG TGTTTGAGGA AGCCCTCATG 
AGCCCTGACA CAAGATACAG GTGCCACCGG AAAGACTCCC TCCCTGATGC AGACTTGGCA 
AGCTGTGGGA GTCTCAGTCA GAGCAGTGCC AGCTTCTTTA CCCCCAGGAG CACCAGGAAT 
GATGAACTAC TCAGTGACCT CACCCGGACT CCTCCACCAC CATCCTCCAC CTTTCCGAAA 
GCTTCCAGCG AGTCCTCTTA TCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA 
GAACAGAGAT CTCAGGAGTT GGCTGCAATG GAAGAAACCC GGATAGTCAT TCTGAACAAC 
CTCGAGGAAC TTAAGCAAAA AATCAAAGAC ATAAATGATC AGATGGATGA GTCTTTCAGA 
GAGTTGGATA TGGAATGTGC TCTTTTGGAT GGAGAACAGA AATCTGAAAC AACTGAACTT 
ATGAAGGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TAGCTGAACT GGAAAAGAAC 
ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG 
AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG 
AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCTGACC TGTTGGATGT TGAAAGCAAA 
CACTTTGAAG ACCTGGAGTT CCAGCAGCTT GAACATGAGA GCCGTCTAGA TGAAGAAAAG 
GAGAACTTGA CTCAACAGCT CCTGCGTGAA GTTGCTGAAT ATCAACGGAA CATCGTTTCT 
AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCCAATC ACATTGTTCA GCAGGCTCAG 
AGAGAGCAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGTT GCAAAGAGAA 
AAGGAGAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT 
CCCGTTAACC CCAATACTTT AAAAGAGGGC TATATCAGTG TAAATGAGAT TAATGAGCCG 
TGTGGCAATT CCACGAATCT ATCCCCTTCC ACTCAGTTTC CTGCTGATGC TGATGCTGTT 
GCCACTGAGC CTGCCACAGC TGTGCTGGCG AGCCAGCCAC AGAGTAAAGA GCACTTTAGA 
AGTCTGGAAG AAAGGAAAAA ACAGCATAAA GAAGGCCTCT ATCTGAGTGA TACTTTGCCT 
CGAAAGAAAA CCACATCTTC CATCTCCCCA CATTTCAGCA GTGCTACTAT GGGGAGAAGC 
ATCACCCCAA AGGCCCATCT GCCCCTAGGA CAGAGTAACA GCTGTGGAAG TGTGCTCCCT 
CCCTCACTGG CAGCCATGGC CAAAGACTCA GAATCTCGGA GGATGCTCAG AGGTTATAAT 
CACCAACAGA TGAGTGAAGG ACACAGGCAG AAATCTGAAT TTTATAACCG CACAGCATCT 
GAATCAAATG TCTACTTGAA TAGTTTCCAT TATCCAGATC ACAGCTACAA GGACCAGGCC 
TTTGATACTC TGAGCCTCGA TAGCTCTGAT AGCATGGAGA CCAGCATCTC TGCTTGCTCA 
CCAGACAACA TCTCTAGTGC CAGCACTTCA AATATTGCTA GAATAGAAGA AATGGAGAGA 
CTTTTGAAGC AGGCTCATGC AGAAAAGACG CGGCTGCTCG AATCCAGGGA ACGGGAAATG 
GAAGCCAAAA AACGAGCCCT GGAAGAAGAA AAACGACGCC GGGAAATCCT GGAAAAACGA 
TTACAGGAAG AAACTAGCCA GAGGCAGAAG TTAATAGAAA AGGAAGTAAA AATAAGGGAG 
AGACAAAGGG CACAGGCTCG TCCTTTGACA CGCTACCTGC CTGTCCGGAA GGAAGACTTT 
GATTTGCGGA GCCATGTAGA GACTGCTGGC CACAATATTG ACACCTGTTA CCATGTATCA 
ATCACAGAGA AGACCTGCCG AGGATTCCTC ATCAAAATGG GTGGGAAAAT TAAAACGTGG 
AAAAAACGTT GGTTTGTTTT TGATCGGAAC AAGCGAACAT TCTCTTATTA TGCAGACAAG 
CATGAAACTA AATTGAAAGG AGTAATATAC TTTCAAGCCA TTGAAGAAGT CTATTATGAT 
CACCTCAAGA ATGCTAATAA GAGTCCTAAT CCGTTACTCA CCTTTAGCGT CAAGACTCAT 
GACAGAATCT ATTATATGGT AGCCCCATCG CCAGAAGCCA TGCGGATCTG GATGGATGTT 
ATAGTTACGG GGGCAGAAGG TTACACTCAC TTCTTGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001127911.1
CDS	67..3828
Translation	MEEHSYIQKELDLQNGSLEEDSVVHSVENDSQNMMESLSPKKYSSSLRFKANGDYSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGSKQFSYDGTDKNIPMKPPTPLLNTTSSLSGYPLGRADFDHYTGRDSERALRLSEKPPYSKYSSRHKSHDNVYSLGGLEGRKASGSLLAMWNGSSLSDAGPPPISRSGAASMPSSPKQARKMSIQDSLALQPKLTRHKELASENINLRTRKYSSSSLSHMGAYSRSLPRLYRATENQLTPLSLPPRNSLGNSKRTKLGEKDLPHSVIDNDNYLNFSSLSSGALPYKTSASEGNPYVSSTLSVPASPRVARKMLLASTSSCASDDFDQASYVGTNPSHSLLAGESDRVFATRRNFSCGSVEFDEADLESLRQASGTPQPALRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRLSTGTTVEDVQKINKELEKLQLSDEESVFEEALMSPDTRYRCHRKDSLPDADLASCGSLSQSSASFFTPRSTRNDELLSDLTRTPPPPSSTFPKASSESSYLSILPKTPEGISEEQRSQELAAMEETRIVILNNLEELKQKIKDINDQMDESFRELDMECALLDGEQKSETTELMKEKEILDHLNRKIAELEKNIVGEKTKEKVKLDAEREKLERLQELYSEQKTQLDNCPESMREQLQQQLKRDADLLDVESKHFEDLEFQQLEHESRLDEEKENLTQQLLREVAEYQRNIVSRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVNPNTLKEGYISVNEINEPCGNSTNLSPSTQFPADADAVATEPATAVLASQPQSKEHFRSLEERKKQHKEGLYLSDTLPRKKTTSSISPHFSSATMGRSITPKAHLPLGQSNSCGSVLPPSLAAMAKDSESRRMLRGYNHQQMSEGHRQKSEFYNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRREILEKRLQEETSQRQKLIEKEVKIRERQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHETKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	NM_001134439.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134439.2

ORF Insert Sequence:

ATGGAAGAGC ATAGCTACAT ACAAAAGGAG CTAGATTTAC AAAATGGTAG CTTAGAGGAA 
GACTCTGTGG TGCATTCTGT TGAGAACGAT TCCCAAAACA TGATGGAGAG CCTCAGCCCA 
AAGAAATACT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTATTCTGG CTCCTATTTA 
ACCCTCTCAC AACCTGTGCC TGCAAAGAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA 
AGCAGCCCCT CCTTAGCCAA AATCCAGGGA AGCAAGCAGT TCTCTTATGA TGGAACTGAC 
AAAAATATTC CTATGAAACC TCCAACTCCT TTACTCAACA CTACATCCTC CCTCAGTGGA 
TATCCACTTG GAAGAGCAGA CTTTGATCAT TATACTGGCC GGGACAGTGA AAGGGCCTTG 
AGGCTCTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGCATAAATC GCATGACAAT 
GTCTACTCTC TTGGAGGGCT GGAAGGTCGG AAGGCATCTG GCTCGCTCCT GGCCATGTGG 
AATGGAAGTT CCCTGAGTGA TGCTGGCCCG CCTCCTATCA GCAGATCGGG AGCCGCAAGC 
ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATGAGCATTC AGGACAGCCT GGCGCTTCAA 
CCCAAGTTAA CTAGACACAA GGAGCTTGCA TCTGAAAACA TCAATTTGAG AACTAGGAAG 
TACTCCAGCA GCAGCCTGAG TCACATGGGA GCCTACAGCC GATCACTTCC CAGGTTGTAC 
AGAGCCACAG AGAACCAGCT GACACCTCTC AGCTTGCCTC CAAGAAACTC TCTGGGCAAT 
TCCAAACGAA CAAAACTTGG GGAAAAGGAT CTACCTCATA GCGTAATAGA CAATGACAAT 
TACCTTAATT TTTCTTCTTT GAGCTCAGGG GCTTTACCCT ATAAAACCTC TGCTTCTGAA 
GGCAATCCTT ATGTAAGTTC TACCCTCAGT GTCCCTGCCA GTCCACGAGT GGCTCGGAAG 
ATGCTTCTGG CCTCCACCTC CTCCTGTGCC TCTGATGACT TTGATCAGGC TTCATATGTG 
GGGACAAACC CGAGTCATTC ACTTCTTGCT GGAGAGTCAG ACAGAGTTTT TGCGACCAGG 
AGGAACTTCT CTTGTGGATC TGTGGAATTT GATGAGGCAG ATTTGGAAAG CCTCAGACAG 
GCCTCAGGAA CCCCCCAGCC TGCCCTTCGG GAACGGAAAA GCAGTATTAG CTCCATTTCA 
GGACGTGATG ACCTGATGGA TTATCACCGG CGGCAGAGGG AGGAAAGACT CAGGGAGCAG 
GAAATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGTCTCTG TGCTGAATAC 
ACAAAGCCTG ACAGTCGCTT ATCTACTGGG ACCACCGTGG AAGATGTGCA GAAAATCAAC 
AAGGAGCTTG AGAAGCTGCA GCTCTCTGAT GAGGAGTCTG TGTTTGAGGA AGCCCTCATG 
AGCCCTGACA CAAGATACAG GTGCCACCGG AAAGACTCCC TCCCTGATGC AGACTTGGCA 
AGCTGTGGGA GTCTCAGTCA GAGCAGTGCC AGCTTCTTTA CCCCCAGGAG CACCAGGAAT 
GATGAACTAC TCAGTGACCT CACCCGGACT CCTCCACCAC CATCCTCCAC CTTTCCGAAA 
GCTTCCAGCG AGTCCTCTTA TCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA 
GAACAGAGAT CTCAGGAGTT GGCTGCAATG GAAGAAACCC GGATAGTCAT TCTGAACAAC 
CTCGAGGAAC TTAAGCAAAA AATCAAAGAC ATAAATGATC AGATGGATGA GTCTTTCAGA 
GAGTTGGATA TGGAATGTGC TCTTTTGGAT GGAGAACAGA AATCTGAAAC AACTGAACTT 
ATGAAGGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TAGCTGAACT GGAAAAGAAC 
ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG 
AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG 
AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCTGACC TGTTGGATGT TGAAAGCAAA 
CACTTTGAAG ACCTGGAGTT CCAGCAGCTT GAACATGAGA GCCGTCTAGA TGAAGAAAAG 
GAGAACTTGA CTCAACAGCT CCTGCGTGAA GTTGCTGAAT ATCAACGGAA CATCGTTTCT 
AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCCAATC ACATTGTTCA GCAGGCTCAG 
AGAGAGCAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGTT GCAAAGAGAA 
AAGGAGAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT 
CCCGTTAACC CCAATACTTT AAAAGAGGGC TATATCAGTG TAAATGAGAT TAATGAGCCG 
TGTGGCAATT CCACGAATCT ATCCCCTTCC ACTCAGTTTC CTGCTGATGC TGATGCTGTT 
GCCACTGAGC CTGCCACAGC TGTGCTGGCG AGCCAGCCAC AGAGTAAAGA GCACTTTAGA 
AGTCTGGAAG AAAGGAAAAA ACAGCATAAA GAAGGCCTCT ATCTGAGTGA TACTTTGCCT 
CGAAAGAAAA CCACATCTTC CATCTCCCCA CATTTCAGCA GTGCTACTAT GGGGAGAAGC 
ATCACCCCAA AGGCCCATCT GCCCCTAGGA CAGAGTAACA GCTGTGGAAG TGTGCTCCCT 
CCCTCACTGG CAGCCATGGC CAAAGACTCA GAATCTCGGA GGATGCTCAG AGGTTATAAT 
CACCAACAGA TGAGTGAAGG ACACAGGCAG AAATCTGAAT TTTATAACCG CACAGCATCT 
GAATCAAATG TCTACTTGAA TAGTTTCCAT TATCCAGATC ACAGCTACAA GGACCAGGCC 
TTTGATACTC TGAGCCTCGA TAGCTCTGAT AGCATGGAGA CCAGCATCTC TGCTTGCTCA 
CCAGACAACA TCTCTAGTGC CAGCACTTCA AATATTGCTA GAATAGAAGA AATGGAGAGA 
CTTTTGAAGC AGGCTCATGC AGAAAAGACG CGGCTGCTCG AATCCAGGGA ACGGGAAATG 
GAAGCCAAAA AACGAGCCCT GGAAGAAGAA AAACGACGCC GGGAAATCCT GGAAAAACGA 
TTACAGGAAG AAACTAGCCA GAGGCAGAAG TTAATAGAAA AGGAAGTAAA AATAAGGGAG 
AGACAAAGGG CACAGGCTCG TCCTTTGACA CGCTACCTGC CTGTCCGGAA GGAAGACTTT 
GATTTGCGGA GCCATGTAGA GACTGCTGGC CACAATATTG ACACCTGTTA CCATGTATCA 
ATCACAGAGA AGACCTGCCG AGGATTCCTC ATCAAAATGG GTGGGAAAAT TAAAACGTGG 
AAAAAACGTT GGTTTGTTTT TGATCGGAAC AAGCGAACAT TCTCTTATTA TGCAGACAAG 
CATGAAACTA AATTGAAAGG AGTAATATAC TTTCAAGCCA TTGAAGAAGT CTATTATGAT 
CACCTCAAGA ATGCTAATAA GAGTCCTAAT CCGTTACTCA CCTTTAGCGT CAAGACTCAT 
GACAGAATCT ATTATATGGT AGCCCCATCG CCAGAAGCCA TGCGGATCTG GATGGATGTT 
ATAGTTACGG GGGCAGAAGG TTACACTCAC TTCTTGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( NM_145753.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_665696.1 Homo sapiens pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant 4, mRNA.

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CloneID	OHu26394
Clone ID Related Accession (Same CDS sequence)	NM_145753.2
Accession Version	NM_145753.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	pleckstrin homology-like domain family B member 2 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA438817.1, BC069194.1, AK315050.1, AC060225.12 and AW264132.1. On Aug 28, 2008 this sequence version replaced NM_145753.1. Transcript Variant: This variant (4) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF506820.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGAGC ATAGCTACAT ACAAAAGGAG CTAGATTTAC AAAATGGTAG CTTAGAGGAA 
GACTCTGTGG TGCATTCTGT TGAGAACGAT TCCCAAAACA TGATGGAGAG CCTCAGCCCA 
AAGAAATACT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTATTCTGG CTCCTATTTA 
ACCCTCTCAC AACCTGTGCC TGCAAAGAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA 
AGCAGCCCCT CCTTAGCCAA AATCCAGGGA AGCAAGCAGT TCTCTTATGA TGGAACTGAC 
AAAAATATTC CTATGAAACC TCCAACTCCT TTACTCAACA CTACATCCTC CCTCAGTGGA 
TATCCACTTG GAAGAGCAGA CTTTGATCAT TATACTGGCC GGGACAGTGA AAGGGCCTTG 
AGGCTCTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGCATAAATC GCATGACAAT 
GTCTACTCTC TTGGAGGGCT GGAAGGTCGG AAGGCATCTG GCTCGCTCCT GGCCATGTGG 
AATGGAAGTT CCCTGAGTGA TGCTGGCCCG CCTCCTATCA GCAGATCGGG AGCCGCAAGC 
ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATGAGCATTC AGGACAGCCT GGCGCTTCAA 
CCCAAGTTAA CTAGACACAA GGAGCTTGCA TCTGAAAACA TCAATTTGAG AACTAGGAAG 
TACTCCAGCA GCAGCCTGAG TCACATGGGA GCCTACAGCC GATCACTTCC CAGGTTGTAC 
AGAGCCACAG AGAACCAGCT GACACCTCTC AGCTTGCCTC CAAGAAACTC TCTGGGCAAT 
TCCAAACGAA CAAAACTTGG GGAAAAGGAT CTACCTCATA GCGTAATAGA CAATGACAAT 
TACCTTAATT TTTCTTCTTT GAGCTCAGGG GCTTTACCCT ATAAAACCTC TGCTTCTGAA 
GGCAATCCTT ATGTAAGTTC TACCCTCAGT GTCCCTGCCA GTCCACGAGT GGCTCGGAAG 
ATGCTTCTGG CCTCCACCTC CTCCTGTGCC TCTGATGACT TTGATCAGGC TTCATATGTG 
GGGACAAACC CGAGTCATTC ACTTCTTGCT GGAGAGTCAG ACAGAGTTTT TGCGACCAGG 
AGGAACTTCT CTTGTGGATC TGTGGAATTT GATGAGGCAG ATTTGGAAAG CCTCAGACAG 
GCCTCAGGAA CCCCCCAGCC TGCCCTTCGG GAACGGAAAA GCAGTATTAG CTCCATTTCA 
GGACGTGATG ACCTGATGGA TTATCACCGG CGGCAGAGGG AGGAAAGACT CAGGGAGCAG 
GAAATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGTCTCTG TGCTGAATAC 
ACAAAGCCTG ACAGTCGCTT ATCTACTGGG ACCACCGTGG AAGATGTGCA GAAAATCAAC 
AAGGAGCTTG AGAAGCTGCA GCTCTCTGAT GAGGAGTCTG TGTTTGAGGA AGCCCTCATG 
AGCCCTGACA CAAGATACAG GTGCCACCGG AAAGACTCCC TCCCTGATGC AGACTTGGCA 
AGCTGTGGGA GTCTCAGTCA GAGCAGTGCC AGCTTCTTTA CCCCCAGGAG CACCAGGAAT 
GATGAACTAC TCAGTGACCT CACCCGGACT CCTCCACCAC CATCCTCCAC CTTTCCGAAA 
GCTTCCAGCG AGTCCTCTTA TCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA 
GAACAGAGAT CTCAGGAGTT GGCTGCAATG GAAGAAACCC GGATAGTCAT TCTGAACAAC 
CTCGAGGAAC TTAAGCAAAA AATCAAAGAC ATAAATGATC AGATGGATGA GTCTTTCAGA 
GAGTTGGATA TGGAATGTGC TCTTTTGGAT GGAGAACAGA AATCTGAAAC AACTGAACTT 
ATGAAGGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TAGCTGAACT GGAAAAGAAC 
ATTGTTGGTG AAAAGACCAA GGATGCTGAC CTGTTGGATG TTGAAAGCAA ACACTTTGAA 
GACCTGGAGT TCCAGCAGCT TGAACATGAG AGCCGTCTAG ATGAAGAAAA GGAGAACTTG 
ACTCAACAGC TCCTGCGTGA AGTTGCTGAA TATCAACGGA ACATCGTTTC TAGAAAGGAA 
AAAATTTCTG CATTGAAAAA GCAAGCCAAT CACATTGTTC AGCAGGCTCA GAGAGAGCAA 
GATCATTTTG TGAAAGAAAA GAATAATTTA ATAATGATGT TGCAAAGAGA AAAGGAGAAT 
CTTTGTAATT TGGAAAAGAA ATACTCCAGC CTCTCTGGGG GGAAAGGGTT TCCCGTTAAC 
CCCAATACTT TAAAAGAGGG CTATATCAGT GTAAATGAGA TTAATGAGCC GTGTGGCAAT 
TCCACGAATC TATCCCCTTC CACTCAGTTT CCTGCTGATG CTGATGCTGT TGCCACTGAG 
CCTGCCACAG CTGTGCTGGC GAGCCAGCCA CAGAGTAAAG AGCACTTTAG AAGTCTGGAA 
GAAAGGAAAA AACAGCATAA AGAAGGCCTC TATCTGAGTG ATACTTTGCC TCGAAAGAAA 
ACCACATCTT CCATCTCCCC ACATTTCAGC AGTGCTACTA TGGGGAGAAG CATCACCCCA 
AAGGCCCATC TGCCCCTAGG ACAGAGTAAC AGCTGTGGAA GTGTGCTCCC TCCCTCACTG 
GCAGCCATGG CCAAAGACTC AGAATCTCGG AGGATGCTCA GAGGTTATAA TCACCAACAG 
ATGAGTGAAG GACACAGGCA GAAATCTGAA TTTTATAACC GCACAGCATC TGAATCAAAT 
GTCTACTTGA ATAGTTTCCA TTATCCAGAT CACAGCTACA AGGACCAGGC CTTTGATACT 
CTGAGCCTCG ATAGCTCTGA TAGCATGGAG ACCAGCATCT CTGCTTGCTC ACCAGACAAC 
ATCTCTAGTG CCAGCACTTC AAATATTGCT AGAATAGAAG AAATGGAGAG ACTTTTGAAG 
CAGGCTCATG CAGAAAAGAC GCGGCTGCTC GAATCCAGGG AACGGGAAAT GGAAGCCAAA 
AAACGAGCCC TGGAAGAAGA AAAACGACGC CGGGAAATCC TGGAAAAACG ATTACAGGAA 
GAAACTAGCC AGAGGCAGAA GTTAATAGAA AAGGAAGTAA AAATAAGGGA GAGACAAAGG 
GCACAGGCTC GTCCTTTGAC ACGCTACCTG CCTGTCCGGA AGGAAGACTT TGATTTGCGG 
AGCCATGTAG AGACTGCTGG CCACAATATT GACACCTGTT ACCATGTATC AATCACAGAG 
AAGACCTGCC GAGGATTCCT CATCAAAATG GGTGGGAAAA TTAAAACGTG GAAAAAACGT 
TGGTTTGTTT TTGATCGGAA CAAGCGAACA TTCTCTTATT ATGCAGACAA GCATGAAACT 
AAATTGAAAG GAGTAATATA CTTTCAAGCC ATTGAAGAAG TCTATTATGA TCACCTCAAG 
AATGCTAATA AGAGTCCTAA TCCGTTACTC ACCTTTAGCG TCAAGACTCA TGACAGAATC 
TATTATATGG TAGCCCCATC GCCAGAAGCC ATGCGGATCT GGATGGATGT TATAGTTACG 
GGGGCAGAAG GTTACACTCA CTTCTTGTTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_665696.1
CDS	412..4044
Translation	MEEHSYIQKELDLQNGSLEEDSVVHSVENDSQNMMESLSPKKYSSSLRFKANGDYSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGSKQFSYDGTDKNIPMKPPTPLLNTTSSLSGYPLGRADFDHYTGRDSERALRLSEKPPYSKYSSRHKSHDNVYSLGGLEGRKASGSLLAMWNGSSLSDAGPPPISRSGAASMPSSPKQARKMSIQDSLALQPKLTRHKELASENINLRTRKYSSSSLSHMGAYSRSLPRLYRATENQLTPLSLPPRNSLGNSKRTKLGEKDLPHSVIDNDNYLNFSSLSSGALPYKTSASEGNPYVSSTLSVPASPRVARKMLLASTSSCASDDFDQASYVGTNPSHSLLAGESDRVFATRRNFSCGSVEFDEADLESLRQASGTPQPALRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRLSTGTTVEDVQKINKELEKLQLSDEESVFEEALMSPDTRYRCHRKDSLPDADLASCGSLSQSSASFFTPRSTRNDELLSDLTRTPPPPSSTFPKASSESSYLSILPKTPEGISEEQRSQELAAMEETRIVILNNLEELKQKIKDINDQMDESFRELDMECALLDGEQKSETTELMKEKEILDHLNRKIAELEKNIVGEKTKDADLLDVESKHFEDLEFQQLEHESRLDEEKENLTQQLLREVAEYQRNIVSRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVNPNTLKEGYISVNEINEPCGNSTNLSPSTQFPADADAVATEPATAVLASQPQSKEHFRSLEERKKQHKEGLYLSDTLPRKKTTSSISPHFSSATMGRSITPKAHLPLGQSNSCGSVLPPSLAAMAKDSESRRMLRGYNHQQMSEGHRQKSEFYNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRREILEKRLQEETSQRQKLIEKEVKIRERQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHETKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	NM_145753.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145753.2

ORF Insert Sequence:

ATGGAAGAGC ATAGCTACAT ACAAAAGGAG CTAGATTTAC AAAATGGTAG CTTAGAGGAA 
GACTCTGTGG TGCATTCTGT TGAGAACGAT TCCCAAAACA TGATGGAGAG CCTCAGCCCA 
AAGAAATACT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTATTCTGG CTCCTATTTA 
ACCCTCTCAC AACCTGTGCC TGCAAAGAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA 
AGCAGCCCCT CCTTAGCCAA AATCCAGGGA AGCAAGCAGT TCTCTTATGA TGGAACTGAC 
AAAAATATTC CTATGAAACC TCCAACTCCT TTACTCAACA CTACATCCTC CCTCAGTGGA 
TATCCACTTG GAAGAGCAGA CTTTGATCAT TATACTGGCC GGGACAGTGA AAGGGCCTTG 
AGGCTCTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGCATAAATC GCATGACAAT 
GTCTACTCTC TTGGAGGGCT GGAAGGTCGG AAGGCATCTG GCTCGCTCCT GGCCATGTGG 
AATGGAAGTT CCCTGAGTGA TGCTGGCCCG CCTCCTATCA GCAGATCGGG AGCCGCAAGC 
ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATGAGCATTC AGGACAGCCT GGCGCTTCAA 
CCCAAGTTAA CTAGACACAA GGAGCTTGCA TCTGAAAACA TCAATTTGAG AACTAGGAAG 
TACTCCAGCA GCAGCCTGAG TCACATGGGA GCCTACAGCC GATCACTTCC CAGGTTGTAC 
AGAGCCACAG AGAACCAGCT GACACCTCTC AGCTTGCCTC CAAGAAACTC TCTGGGCAAT 
TCCAAACGAA CAAAACTTGG GGAAAAGGAT CTACCTCATA GCGTAATAGA CAATGACAAT 
TACCTTAATT TTTCTTCTTT GAGCTCAGGG GCTTTACCCT ATAAAACCTC TGCTTCTGAA 
GGCAATCCTT ATGTAAGTTC TACCCTCAGT GTCCCTGCCA GTCCACGAGT GGCTCGGAAG 
ATGCTTCTGG CCTCCACCTC CTCCTGTGCC TCTGATGACT TTGATCAGGC TTCATATGTG 
GGGACAAACC CGAGTCATTC ACTTCTTGCT GGAGAGTCAG ACAGAGTTTT TGCGACCAGG 
AGGAACTTCT CTTGTGGATC TGTGGAATTT GATGAGGCAG ATTTGGAAAG CCTCAGACAG 
GCCTCAGGAA CCCCCCAGCC TGCCCTTCGG GAACGGAAAA GCAGTATTAG CTCCATTTCA 
GGACGTGATG ACCTGATGGA TTATCACCGG CGGCAGAGGG AGGAAAGACT CAGGGAGCAG 
GAAATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGTCTCTG TGCTGAATAC 
ACAAAGCCTG ACAGTCGCTT ATCTACTGGG ACCACCGTGG AAGATGTGCA GAAAATCAAC 
AAGGAGCTTG AGAAGCTGCA GCTCTCTGAT GAGGAGTCTG TGTTTGAGGA AGCCCTCATG 
AGCCCTGACA CAAGATACAG GTGCCACCGG AAAGACTCCC TCCCTGATGC AGACTTGGCA 
AGCTGTGGGA GTCTCAGTCA GAGCAGTGCC AGCTTCTTTA CCCCCAGGAG CACCAGGAAT 
GATGAACTAC TCAGTGACCT CACCCGGACT CCTCCACCAC CATCCTCCAC CTTTCCGAAA 
GCTTCCAGCG AGTCCTCTTA TCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA 
GAACAGAGAT CTCAGGAGTT GGCTGCAATG GAAGAAACCC GGATAGTCAT TCTGAACAAC 
CTCGAGGAAC TTAAGCAAAA AATCAAAGAC ATAAATGATC AGATGGATGA GTCTTTCAGA 
GAGTTGGATA TGGAATGTGC TCTTTTGGAT GGAGAACAGA AATCTGAAAC AACTGAACTT 
ATGAAGGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TAGCTGAACT GGAAAAGAAC 
ATTGTTGGTG AAAAGACCAA GGATGCTGAC CTGTTGGATG TTGAAAGCAA ACACTTTGAA 
GACCTGGAGT TCCAGCAGCT TGAACATGAG AGCCGTCTAG ATGAAGAAAA GGAGAACTTG 
ACTCAACAGC TCCTGCGTGA AGTTGCTGAA TATCAACGGA ACATCGTTTC TAGAAAGGAA 
AAAATTTCTG CATTGAAAAA GCAAGCCAAT CACATTGTTC AGCAGGCTCA GAGAGAGCAA 
GATCATTTTG TGAAAGAAAA GAATAATTTA ATAATGATGT TGCAAAGAGA AAAGGAGAAT 
CTTTGTAATT TGGAAAAGAA ATACTCCAGC CTCTCTGGGG GGAAAGGGTT TCCCGTTAAC 
CCCAATACTT TAAAAGAGGG CTATATCAGT GTAAATGAGA TTAATGAGCC GTGTGGCAAT 
TCCACGAATC TATCCCCTTC CACTCAGTTT CCTGCTGATG CTGATGCTGT TGCCACTGAG 
CCTGCCACAG CTGTGCTGGC GAGCCAGCCA CAGAGTAAAG AGCACTTTAG AAGTCTGGAA 
GAAAGGAAAA AACAGCATAA AGAAGGCCTC TATCTGAGTG ATACTTTGCC TCGAAAGAAA 
ACCACATCTT CCATCTCCCC ACATTTCAGC AGTGCTACTA TGGGGAGAAG CATCACCCCA 
AAGGCCCATC TGCCCCTAGG ACAGAGTAAC AGCTGTGGAA GTGTGCTCCC TCCCTCACTG 
GCAGCCATGG CCAAAGACTC AGAATCTCGG AGGATGCTCA GAGGTTATAA TCACCAACAG 
ATGAGTGAAG GACACAGGCA GAAATCTGAA TTTTATAACC GCACAGCATC TGAATCAAAT 
GTCTACTTGA ATAGTTTCCA TTATCCAGAT CACAGCTACA AGGACCAGGC CTTTGATACT 
CTGAGCCTCG ATAGCTCTGA TAGCATGGAG ACCAGCATCT CTGCTTGCTC ACCAGACAAC 
ATCTCTAGTG CCAGCACTTC AAATATTGCT AGAATAGAAG AAATGGAGAG ACTTTTGAAG 
CAGGCTCATG CAGAAAAGAC GCGGCTGCTC GAATCCAGGG AACGGGAAAT GGAAGCCAAA 
AAACGAGCCC TGGAAGAAGA AAAACGACGC CGGGAAATCC TGGAAAAACG ATTACAGGAA 
GAAACTAGCC AGAGGCAGAA GTTAATAGAA AAGGAAGTAA AAATAAGGGA GAGACAAAGG 
GCACAGGCTC GTCCTTTGAC ACGCTACCTG CCTGTCCGGA AGGAAGACTT TGATTTGCGG 
AGCCATGTAG AGACTGCTGG CCACAATATT GACACCTGTT ACCATGTATC AATCACAGAG 
AAGACCTGCC GAGGATTCCT CATCAAAATG GGTGGGAAAA TTAAAACGTG GAAAAAACGT 
TGGTTTGTTT TTGATCGGAA CAAGCGAACA TTCTCTTATT ATGCAGACAA GCATGAAACT 
AAATTGAAAG GAGTAATATA CTTTCAAGCC ATTGAAGAAG TCTATTATGA TCACCTCAAG 
AATGCTAATA AGAGTCCTAA TCCGTTACTC ACCTTTAGCG TCAAGACTCA TGACAGAATC 
TATTATATGG TAGCCCCATC GCCAGAAGCC ATGCGGATCT GGATGGATGT TATAGTTACG 
GGGGCAGAAG GTTACACTCA CTTCTTGTTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( NM_001134437.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001127909.1 Homo sapiens pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu27088
Clone ID Related Accession (Same CDS sequence)	NM_001134437.2 , NM_001134437.1
Accession Version	NM_001134437.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-31
Organism	Homo sapiens(human)
Product	pleckstrin homology-like domain family B member 2 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA447763.1, CR749654.1, AF506820.1, CA310697.1, AC060225.12 and AW264132.1. On Jun 1, 2019 this sequence version replaced NM_001134437.1. Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has a longer N-terminus and lacks an alternate internal segment compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR749654.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGG AGGATACCAA GAGAGAGGTG CCCAAGGAAG ATGGAGTTGG TGATGTGCAA 
CATTTCGATT CCAGCAAGAT TATGGAAGAG CATAGCTACA TACAAAAGGA GCTAGATTTA 
CAAAATGGTA GCTTAGAGGA AGACTCTGTG GTGCATTCTG TTGAGAACGA TTCCCAAAAC 
ATGATGGAGA GCCTCAGCCC AAAGAAATAC TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTATTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCAAAGAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGCAGCCCC TCCTTAGCCA AAATCCAGGG AAGCAAGCAG 
TTCTCTTATG ATGGAACTGA CAAAAATATT CCTATGAAAC CTCCAACTCC TTTACTCAAC 
ACTACATCCT CCCTCAGTGG ATATCCACTT GGAAGAGCAG ACTTTGATCA TTATACTGGC 
CGGGACAGTG AAAGGGCCTT GAGGCTCTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGCATAAAT CGCATGACAA TGTCTACTCT CTTGGAGGGC TGGAAGGTCG GAAGGCATCT 
GGCTCGCTCC TGGCCATGTG GAATGGAAGT TCCCTGAGTG ATGCTGGCCC GCCTCCTATC 
AGCAGATCGG GAGCCGCAAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATGAGCATT 
CAGGACAGCC TGGCGCTTCA ACCCAAGTTA ACTAGACACA AGGAGCTTGC ATCTGAAAAC 
ATCAATTTGA GAACTAGGAA GTACTCCAGC AGCAGCCTGA GTCACATGGG AGCCTACAGC 
CGATCACTTC CCAGGTTGTA CAGAGCCACA GAGAACCAGC TGACACCTCT CAGCTTGCCT 
CCAAGAAACT CTCTGGGCAA TTCCAAACGA ACAAAACTTG GGGAAAAGGA TCTACCTCAT 
AGCGTAATAG ACAATGACAA TTACCTTAAT TTTTCTTCTT TGAGCTCAGG GGCTTTACCC 
TATAAAACCT CTGCTTCTGA AGGCAATCCT TATGTAAGTT CTACCCTCAG TGTCCCTGCC 
AGTCCACGAG TGGCTCGGAA GATGCTTCTG GCCTCCACCT CCTCCTGTGC CTCTGATGAC 
TTTGATCAGG CTTCATATGT GGGGACAAAC CCGAGTCATT CACTTCTTGC TGGAGAGTCA 
GACAGAGTTT TTGCGACCAG GAGGAACTTC TCTTGTGGAT CTGTGGAATT TGATGAGGCA 
GATTTGGAAA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGCCCTTCG GGAACGGAAA 
AGCAGTATTA GCTCCATTTC AGGACGTGAT GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAAAGAC TCAGGGAGCA GGAAATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGTCTCT GTGCTGAATA CACAAAGCCT GACAGTCGCT TATCTACTGG GACCACCGTG 
GAAGATGTGC AGAAAATCAA CAAGGAGCTT GAGAAGCTGC AGCTCTCTGA TGAGGAGTCT 
GTGTTTGAGG AAGCCCTCAT GAGCCCTGAC ACAAGATACA GGTGCCACCG GAAAGACTCC 
CTCCCTGATG CAGACTTGGC AAGCTGTGGG AGTCTCAGTC AGAGCAGTGC CAGCTTCTTT 
ACCCCCAGGA GCACCAGGAA TGATGAACTA CTCAGTGACC TCACCCGGAC TCCTCCACCA 
CCATCCTCCA CCTTTCCGAA AGCTTCCAGC GAGTCCTCTT ATCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAGAGA TCTCAGGAGT TGGCTGCAAT GGAAGAAACC 
CGGATAGTCA TTCTGAACAA CCTCGAGGAA CTTAAGCAAA AAATCAAAGA CATAAATGAT 
CAGATGGATG AGTCTTTCAG AGAGTTGGAT ATGGAATGTG CTCTTTTGGA TGGAGAACAG 
AAATCTGAAA CAACTGAACT TATGAAGGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATAGCTGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGATGCTGA CCTGTTGGAT 
GTTGAAAGCA AACACTTTGA AGACCTGGAG TTCCAGCAGC TTGAACATGA GAGCCGTCTA 
GATGAAGAAA AGGAGAACTT GACTCAACAG CTCCTGCGTG AAGTTGCTGA ATATCAACGG 
AACATCGTTT CTAGAAAGGA AAAAATTTCT GCATTGAAAA AGCAAGCCAA TCACATTGTT 
CAGCAGGCTC AGAGAGAGCA AGATCATTTT GTGAAAGAAA AGAATAATTT AATAATGATG 
TTGCAAAGAG AAAAGGAGAA TCTTTGTAAT TTGGAAAAGA AATACTCCAG CCTCTCTGGG 
GGGAAAGGGT TTCCCGTTAA CCCCAATACT TTAAAAGAGG GCTATATCAG TGTAAATGAG 
ATTAATGAGC CGTGTGGCAA TTCCACGAAT CTATCCCCTT CCACTCAGTT TCCTGCTGAT 
GCTGATGCTG TTGCCACTGA GCCTGCCACA GCTGTGCTGG CGAGCCAGCC ACAGAGTAAA 
GAGCACTTTA GAAGTCTGGA AGAAAGGAAA AAACAGCATA AAGAAGGCCT CTATCTGAGT 
GATACTTTGC CTCGAAAGAA AACCACATCT TCCATCTCCC CACATTTCAG CAGTGCTACT 
ATGGGGAGAA GCATCACCCC AAAGGCCCAT CTGCCCCTAG GACAGAGTAA CAGCTGTGGA 
AGTGTGCTCC CTCCCTCACT GGCAGCCATG GCCAAAGACT CAGAATCTCG GAGGATGCTC 
AGAGGTTATA ATCACCAACA GATGAGTGAA GGACACAGGC AGAAATCTGA ATTTTATAAC 
CGCACAGCAT CTGAATCAAA TGTCTACTTG AATAGTTTCC ATTATCCAGA TCACAGCTAC 
AAGGACCAGG CCTTTGATAC TCTGAGCCTC GATAGCTCTG ATAGCATGGA GACCAGCATC 
TCTGCTTGCT CACCAGACAA CATCTCTAGT GCCAGCACTT CAAATATTGC TAGAATAGAA 
GAAATGGAGA GACTTTTGAA GCAGGCTCAT GCAGAAAAGA CGCGGCTGCT CGAATCCAGG 
GAACGGGAAA TGGAAGCCAA AAAACGAGCC CTGGAAGAAG AAAAACGACG CCGGGAAATC 
CTGGAAAAAC GATTACAGGA AGAAACTAGC CAGAGGCAGA AGTTAATAGA AAAGGAAGTA 
AAAATAAGGG AGAGACAAAG GGCACAGGCT CGTCCTTTGA CACGCTACCT GCCTGTCCGG 
AAGGAAGACT TTGATTTGCG GAGCCATGTA GAGACTGCTG GCCACAATAT TGACACCTGT 
TACCATGTAT CAATCACAGA GAAGACCTGC CGAGGATTCC TCATCAAAAT GGGTGGGAAA 
ATTAAAACGT GGAAAAAACG TTGGTTTGTT TTTGATCGGA ACAAGCGAAC ATTCTCTTAT 
TATGCAGACA AGCATGAAAC TAAATTGAAA GGAGTAATAT ACTTTCAAGC CATTGAAGAA 
GTCTATTATG ATCACCTCAA GAATGCTAAT AAGAGTCCTA ATCCGTTACT CACCTTTAGC 
GTCAAGACTC ATGACAGAAT CTATTATATG GTAGCCCCAT CGCCAGAAGC CATGCGGATC 
TGGATGGATG TTATAGTTAC GGGGGCAGAA GGTTACACTC ACTTCTTGTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001127909.1
CDS	257..3970
Translation	MEEEDTKREVPKEDGVGDVQHFDSSKIMEEHSYIQKELDLQNGSLEEDSVVHSVENDSQNMMESLSPKKYSSSLRFKANGDYSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGSKQFSYDGTDKNIPMKPPTPLLNTTSSLSGYPLGRADFDHYTGRDSERALRLSEKPPYSKYSSRHKSHDNVYSLGGLEGRKASGSLLAMWNGSSLSDAGPPPISRSGAASMPSSPKQARKMSIQDSLALQPKLTRHKELASENINLRTRKYSSSSLSHMGAYSRSLPRLYRATENQLTPLSLPPRNSLGNSKRTKLGEKDLPHSVIDNDNYLNFSSLSSGALPYKTSASEGNPYVSSTLSVPASPRVARKMLLASTSSCASDDFDQASYVGTNPSHSLLAGESDRVFATRRNFSCGSVEFDEADLESLRQASGTPQPALRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRLSTGTTVEDVQKINKELEKLQLSDEESVFEEALMSPDTRYRCHRKDSLPDADLASCGSLSQSSASFFTPRSTRNDELLSDLTRTPPPPSSTFPKASSESSYLSILPKTPEGISEEQRSQELAAMEETRIVILNNLEELKQKIKDINDQMDESFRELDMECALLDGEQKSETTELMKEKEILDHLNRKIAELEKNIVGEKTKDADLLDVESKHFEDLEFQQLEHESRLDEEKENLTQQLLREVAEYQRNIVSRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVNPNTLKEGYISVNEINEPCGNSTNLSPSTQFPADADAVATEPATAVLASQPQSKEHFRSLEERKKQHKEGLYLSDTLPRKKTTSSISPHFSSATMGRSITPKAHLPLGQSNSCGSVLPPSLAAMAKDSESRRMLRGYNHQQMSEGHRQKSEFYNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRREILEKRLQEETSQRQKLIEKEVKIRERQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHETKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	NM_001134437.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134437.2

ORF Insert Sequence:

ATGGAGGAGG AGGATACCAA GAGAGAGGTG CCCAAGGAAG ATGGAGTTGG TGATGTGCAA 
CATTTCGATT CCAGCAAGAT TATGGAAGAG CATAGCTACA TACAAAAGGA GCTAGATTTA 
CAAAATGGTA GCTTAGAGGA AGACTCTGTG GTGCATTCTG TTGAGAACGA TTCCCAAAAC 
ATGATGGAGA GCCTCAGCCC AAAGAAATAC TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTATTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCAAAGAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGCAGCCCC TCCTTAGCCA AAATCCAGGG AAGCAAGCAG 
TTCTCTTATG ATGGAACTGA CAAAAATATT CCTATGAAAC CTCCAACTCC TTTACTCAAC 
ACTACATCCT CCCTCAGTGG ATATCCACTT GGAAGAGCAG ACTTTGATCA TTATACTGGC 
CGGGACAGTG AAAGGGCCTT GAGGCTCTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGCATAAAT CGCATGACAA TGTCTACTCT CTTGGAGGGC TGGAAGGTCG GAAGGCATCT 
GGCTCGCTCC TGGCCATGTG GAATGGAAGT TCCCTGAGTG ATGCTGGCCC GCCTCCTATC 
AGCAGATCGG GAGCCGCAAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATGAGCATT 
CAGGACAGCC TGGCGCTTCA ACCCAAGTTA ACTAGACACA AGGAGCTTGC ATCTGAAAAC 
ATCAATTTGA GAACTAGGAA GTACTCCAGC AGCAGCCTGA GTCACATGGG AGCCTACAGC 
CGATCACTTC CCAGGTTGTA CAGAGCCACA GAGAACCAGC TGACACCTCT CAGCTTGCCT 
CCAAGAAACT CTCTGGGCAA TTCCAAACGA ACAAAACTTG GGGAAAAGGA TCTACCTCAT 
AGCGTAATAG ACAATGACAA TTACCTTAAT TTTTCTTCTT TGAGCTCAGG GGCTTTACCC 
TATAAAACCT CTGCTTCTGA AGGCAATCCT TATGTAAGTT CTACCCTCAG TGTCCCTGCC 
AGTCCACGAG TGGCTCGGAA GATGCTTCTG GCCTCCACCT CCTCCTGTGC CTCTGATGAC 
TTTGATCAGG CTTCATATGT GGGGACAAAC CCGAGTCATT CACTTCTTGC TGGAGAGTCA 
GACAGAGTTT TTGCGACCAG GAGGAACTTC TCTTGTGGAT CTGTGGAATT TGATGAGGCA 
GATTTGGAAA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGCCCTTCG GGAACGGAAA 
AGCAGTATTA GCTCCATTTC AGGACGTGAT GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAAAGAC TCAGGGAGCA GGAAATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGTCTCT GTGCTGAATA CACAAAGCCT GACAGTCGCT TATCTACTGG GACCACCGTG 
GAAGATGTGC AGAAAATCAA CAAGGAGCTT GAGAAGCTGC AGCTCTCTGA TGAGGAGTCT 
GTGTTTGAGG AAGCCCTCAT GAGCCCTGAC ACAAGATACA GGTGCCACCG GAAAGACTCC 
CTCCCTGATG CAGACTTGGC AAGCTGTGGG AGTCTCAGTC AGAGCAGTGC CAGCTTCTTT 
ACCCCCAGGA GCACCAGGAA TGATGAACTA CTCAGTGACC TCACCCGGAC TCCTCCACCA 
CCATCCTCCA CCTTTCCGAA AGCTTCCAGC GAGTCCTCTT ATCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAGAGA TCTCAGGAGT TGGCTGCAAT GGAAGAAACC 
CGGATAGTCA TTCTGAACAA CCTCGAGGAA CTTAAGCAAA AAATCAAAGA CATAAATGAT 
CAGATGGATG AGTCTTTCAG AGAGTTGGAT ATGGAATGTG CTCTTTTGGA TGGAGAACAG 
AAATCTGAAA CAACTGAACT TATGAAGGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATAGCTGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGATGCTGA CCTGTTGGAT 
GTTGAAAGCA AACACTTTGA AGACCTGGAG TTCCAGCAGC TTGAACATGA GAGCCGTCTA 
GATGAAGAAA AGGAGAACTT GACTCAACAG CTCCTGCGTG AAGTTGCTGA ATATCAACGG 
AACATCGTTT CTAGAAAGGA AAAAATTTCT GCATTGAAAA AGCAAGCCAA TCACATTGTT 
CAGCAGGCTC AGAGAGAGCA AGATCATTTT GTGAAAGAAA AGAATAATTT AATAATGATG 
TTGCAAAGAG AAAAGGAGAA TCTTTGTAAT TTGGAAAAGA AATACTCCAG CCTCTCTGGG 
GGGAAAGGGT TTCCCGTTAA CCCCAATACT TTAAAAGAGG GCTATATCAG TGTAAATGAG 
ATTAATGAGC CGTGTGGCAA TTCCACGAAT CTATCCCCTT CCACTCAGTT TCCTGCTGAT 
GCTGATGCTG TTGCCACTGA GCCTGCCACA GCTGTGCTGG CGAGCCAGCC ACAGAGTAAA 
GAGCACTTTA GAAGTCTGGA AGAAAGGAAA AAACAGCATA AAGAAGGCCT CTATCTGAGT 
GATACTTTGC CTCGAAAGAA AACCACATCT TCCATCTCCC CACATTTCAG CAGTGCTACT 
ATGGGGAGAA GCATCACCCC AAAGGCCCAT CTGCCCCTAG GACAGAGTAA CAGCTGTGGA 
AGTGTGCTCC CTCCCTCACT GGCAGCCATG GCCAAAGACT CAGAATCTCG GAGGATGCTC 
AGAGGTTATA ATCACCAACA GATGAGTGAA GGACACAGGC AGAAATCTGA ATTTTATAAC 
CGCACAGCAT CTGAATCAAA TGTCTACTTG AATAGTTTCC ATTATCCAGA TCACAGCTAC 
AAGGACCAGG CCTTTGATAC TCTGAGCCTC GATAGCTCTG ATAGCATGGA GACCAGCATC 
TCTGCTTGCT CACCAGACAA CATCTCTAGT GCCAGCACTT CAAATATTGC TAGAATAGAA 
GAAATGGAGA GACTTTTGAA GCAGGCTCAT GCAGAAAAGA CGCGGCTGCT CGAATCCAGG 
GAACGGGAAA TGGAAGCCAA AAAACGAGCC CTGGAAGAAG AAAAACGACG CCGGGAAATC 
CTGGAAAAAC GATTACAGGA AGAAACTAGC CAGAGGCAGA AGTTAATAGA AAAGGAAGTA 
AAAATAAGGG AGAGACAAAG GGCACAGGCT CGTCCTTTGA CACGCTACCT GCCTGTCCGG 
AAGGAAGACT TTGATTTGCG GAGCCATGTA GAGACTGCTG GCCACAATAT TGACACCTGT 
TACCATGTAT CAATCACAGA GAAGACCTGC CGAGGATTCC TCATCAAAAT GGGTGGGAAA 
ATTAAAACGT GGAAAAAACG TTGGTTTGTT TTTGATCGGA ACAAGCGAAC ATTCTCTTAT 
TATGCAGACA AGCATGAAAC TAAATTGAAA GGAGTAATAT ACTTTCAAGC CATTGAAGAA 
GTCTATTATG ATCACCTCAA GAATGCTAAT AAGAGTCCTA ATCCGTTACT CACCTTTAGC 
GTCAAGACTC ATGACAGAAT CTATTATATG GTAGCCCCAT CGCCAGAAGC CATGCGGATC 
TGGATGGATG TTATAGTTAC GGGGGCAGAA GGTTACACTC ACTTCTTGTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( NM_001134437.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001127909.1 Homo sapiens pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant 3, mRNA.

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CloneID	OHu27088
Clone ID Related Accession (Same CDS sequence)	NM_001134437.2 , NM_001134437.1
Accession Version	NM_001134437.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	pleckstrin homology-like domain family B member 2 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA447763.1, CR749654.1, AF506820.1, CA310697.1, AC060225.12 and AW264132.1. Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has a longer N-terminus and lacks an alternate internal segment compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR749654.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGG AGGATACCAA GAGAGAGGTG CCCAAGGAAG ATGGAGTTGG TGATGTGCAA 
CATTTCGATT CCAGCAAGAT TATGGAAGAG CATAGCTACA TACAAAAGGA GCTAGATTTA 
CAAAATGGTA GCTTAGAGGA AGACTCTGTG GTGCATTCTG TTGAGAACGA TTCCCAAAAC 
ATGATGGAGA GCCTCAGCCC AAAGAAATAC TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTATTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCAAAGAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGCAGCCCC TCCTTAGCCA AAATCCAGGG AAGCAAGCAG 
TTCTCTTATG ATGGAACTGA CAAAAATATT CCTATGAAAC CTCCAACTCC TTTACTCAAC 
ACTACATCCT CCCTCAGTGG ATATCCACTT GGAAGAGCAG ACTTTGATCA TTATACTGGC 
CGGGACAGTG AAAGGGCCTT GAGGCTCTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGCATAAAT CGCATGACAA TGTCTACTCT CTTGGAGGGC TGGAAGGTCG GAAGGCATCT 
GGCTCGCTCC TGGCCATGTG GAATGGAAGT TCCCTGAGTG ATGCTGGCCC GCCTCCTATC 
AGCAGATCGG GAGCCGCAAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATGAGCATT 
CAGGACAGCC TGGCGCTTCA ACCCAAGTTA ACTAGACACA AGGAGCTTGC ATCTGAAAAC 
ATCAATTTGA GAACTAGGAA GTACTCCAGC AGCAGCCTGA GTCACATGGG AGCCTACAGC 
CGATCACTTC CCAGGTTGTA CAGAGCCACA GAGAACCAGC TGACACCTCT CAGCTTGCCT 
CCAAGAAACT CTCTGGGCAA TTCCAAACGA ACAAAACTTG GGGAAAAGGA TCTACCTCAT 
AGCGTAATAG ACAATGACAA TTACCTTAAT TTTTCTTCTT TGAGCTCAGG GGCTTTACCC 
TATAAAACCT CTGCTTCTGA AGGCAATCCT TATGTAAGTT CTACCCTCAG TGTCCCTGCC 
AGTCCACGAG TGGCTCGGAA GATGCTTCTG GCCTCCACCT CCTCCTGTGC CTCTGATGAC 
TTTGATCAGG CTTCATATGT GGGGACAAAC CCGAGTCATT CACTTCTTGC TGGAGAGTCA 
GACAGAGTTT TTGCGACCAG GAGGAACTTC TCTTGTGGAT CTGTGGAATT TGATGAGGCA 
GATTTGGAAA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGCCCTTCG GGAACGGAAA 
AGCAGTATTA GCTCCATTTC AGGACGTGAT GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAAAGAC TCAGGGAGCA GGAAATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGTCTCT GTGCTGAATA CACAAAGCCT GACAGTCGCT TATCTACTGG GACCACCGTG 
GAAGATGTGC AGAAAATCAA CAAGGAGCTT GAGAAGCTGC AGCTCTCTGA TGAGGAGTCT 
GTGTTTGAGG AAGCCCTCAT GAGCCCTGAC ACAAGATACA GGTGCCACCG GAAAGACTCC 
CTCCCTGATG CAGACTTGGC AAGCTGTGGG AGTCTCAGTC AGAGCAGTGC CAGCTTCTTT 
ACCCCCAGGA GCACCAGGAA TGATGAACTA CTCAGTGACC TCACCCGGAC TCCTCCACCA 
CCATCCTCCA CCTTTCCGAA AGCTTCCAGC GAGTCCTCTT ATCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAGAGA TCTCAGGAGT TGGCTGCAAT GGAAGAAACC 
CGGATAGTCA TTCTGAACAA CCTCGAGGAA CTTAAGCAAA AAATCAAAGA CATAAATGAT 
CAGATGGATG AGTCTTTCAG AGAGTTGGAT ATGGAATGTG CTCTTTTGGA TGGAGAACAG 
AAATCTGAAA CAACTGAACT TATGAAGGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATAGCTGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGATGCTGA CCTGTTGGAT 
GTTGAAAGCA AACACTTTGA AGACCTGGAG TTCCAGCAGC TTGAACATGA GAGCCGTCTA 
GATGAAGAAA AGGAGAACTT GACTCAACAG CTCCTGCGTG AAGTTGCTGA ATATCAACGG 
AACATCGTTT CTAGAAAGGA AAAAATTTCT GCATTGAAAA AGCAAGCCAA TCACATTGTT 
CAGCAGGCTC AGAGAGAGCA AGATCATTTT GTGAAAGAAA AGAATAATTT AATAATGATG 
TTGCAAAGAG AAAAGGAGAA TCTTTGTAAT TTGGAAAAGA AATACTCCAG CCTCTCTGGG 
GGGAAAGGGT TTCCCGTTAA CCCCAATACT TTAAAAGAGG GCTATATCAG TGTAAATGAG 
ATTAATGAGC CGTGTGGCAA TTCCACGAAT CTATCCCCTT CCACTCAGTT TCCTGCTGAT 
GCTGATGCTG TTGCCACTGA GCCTGCCACA GCTGTGCTGG CGAGCCAGCC ACAGAGTAAA 
GAGCACTTTA GAAGTCTGGA AGAAAGGAAA AAACAGCATA AAGAAGGCCT CTATCTGAGT 
GATACTTTGC CTCGAAAGAA AACCACATCT TCCATCTCCC CACATTTCAG CAGTGCTACT 
ATGGGGAGAA GCATCACCCC AAAGGCCCAT CTGCCCCTAG GACAGAGTAA CAGCTGTGGA 
AGTGTGCTCC CTCCCTCACT GGCAGCCATG GCCAAAGACT CAGAATCTCG GAGGATGCTC 
AGAGGTTATA ATCACCAACA GATGAGTGAA GGACACAGGC AGAAATCTGA ATTTTATAAC 
CGCACAGCAT CTGAATCAAA TGTCTACTTG AATAGTTTCC ATTATCCAGA TCACAGCTAC 
AAGGACCAGG CCTTTGATAC TCTGAGCCTC GATAGCTCTG ATAGCATGGA GACCAGCATC 
TCTGCTTGCT CACCAGACAA CATCTCTAGT GCCAGCACTT CAAATATTGC TAGAATAGAA 
GAAATGGAGA GACTTTTGAA GCAGGCTCAT GCAGAAAAGA CGCGGCTGCT CGAATCCAGG 
GAACGGGAAA TGGAAGCCAA AAAACGAGCC CTGGAAGAAG AAAAACGACG CCGGGAAATC 
CTGGAAAAAC GATTACAGGA AGAAACTAGC CAGAGGCAGA AGTTAATAGA AAAGGAAGTA 
AAAATAAGGG AGAGACAAAG GGCACAGGCT CGTCCTTTGA CACGCTACCT GCCTGTCCGG 
AAGGAAGACT TTGATTTGCG GAGCCATGTA GAGACTGCTG GCCACAATAT TGACACCTGT 
TACCATGTAT CAATCACAGA GAAGACCTGC CGAGGATTCC TCATCAAAAT GGGTGGGAAA 
ATTAAAACGT GGAAAAAACG TTGGTTTGTT TTTGATCGGA ACAAGCGAAC ATTCTCTTAT 
TATGCAGACA AGCATGAAAC TAAATTGAAA GGAGTAATAT ACTTTCAAGC CATTGAAGAA 
GTCTATTATG ATCACCTCAA GAATGCTAAT AAGAGTCCTA ATCCGTTACT CACCTTTAGC 
GTCAAGACTC ATGACAGAAT CTATTATATG GTAGCCCCAT CGCCAGAAGC CATGCGGATC 
TGGATGGATG TTATAGTTAC GGGGGCAGAA GGTTACACTC ACTTCTTGTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001127909.1
CDS	273..3986
Translation	MEEEDTKREVPKEDGVGDVQHFDSSKIMEEHSYIQKELDLQNGSLEEDSVVHSVENDSQNMMESLSPKKYSSSLRFKANGDYSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGSKQFSYDGTDKNIPMKPPTPLLNTTSSLSGYPLGRADFDHYTGRDSERALRLSEKPPYSKYSSRHKSHDNVYSLGGLEGRKASGSLLAMWNGSSLSDAGPPPISRSGAASMPSSPKQARKMSIQDSLALQPKLTRHKELASENINLRTRKYSSSSLSHMGAYSRSLPRLYRATENQLTPLSLPPRNSLGNSKRTKLGEKDLPHSVIDNDNYLNFSSLSSGALPYKTSASEGNPYVSSTLSVPASPRVARKMLLASTSSCASDDFDQASYVGTNPSHSLLAGESDRVFATRRNFSCGSVEFDEADLESLRQASGTPQPALRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRLSTGTTVEDVQKINKELEKLQLSDEESVFEEALMSPDTRYRCHRKDSLPDADLASCGSLSQSSASFFTPRSTRNDELLSDLTRTPPPPSSTFPKASSESSYLSILPKTPEGISEEQRSQELAAMEETRIVILNNLEELKQKIKDINDQMDESFRELDMECALLDGEQKSETTELMKEKEILDHLNRKIAELEKNIVGEKTKDADLLDVESKHFEDLEFQQLEHESRLDEEKENLTQQLLREVAEYQRNIVSRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVNPNTLKEGYISVNEINEPCGNSTNLSPSTQFPADADAVATEPATAVLASQPQSKEHFRSLEERKKQHKEGLYLSDTLPRKKTTSSISPHFSSATMGRSITPKAHLPLGQSNSCGSVLPPSLAAMAKDSESRRMLRGYNHQQMSEGHRQKSEFYNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRREILEKRLQEETSQRQKLIEKEVKIRERQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHETKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	NM_001134437.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134437.1

ORF Insert Sequence:

ATGGAGGAGG AGGATACCAA GAGAGAGGTG CCCAAGGAAG ATGGAGTTGG TGATGTGCAA 
CATTTCGATT CCAGCAAGAT TATGGAAGAG CATAGCTACA TACAAAAGGA GCTAGATTTA 
CAAAATGGTA GCTTAGAGGA AGACTCTGTG GTGCATTCTG TTGAGAACGA TTCCCAAAAC 
ATGATGGAGA GCCTCAGCCC AAAGAAATAC TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTATTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCAAAGAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGCAGCCCC TCCTTAGCCA AAATCCAGGG AAGCAAGCAG 
TTCTCTTATG ATGGAACTGA CAAAAATATT CCTATGAAAC CTCCAACTCC TTTACTCAAC 
ACTACATCCT CCCTCAGTGG ATATCCACTT GGAAGAGCAG ACTTTGATCA TTATACTGGC 
CGGGACAGTG AAAGGGCCTT GAGGCTCTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGCATAAAT CGCATGACAA TGTCTACTCT CTTGGAGGGC TGGAAGGTCG GAAGGCATCT 
GGCTCGCTCC TGGCCATGTG GAATGGAAGT TCCCTGAGTG ATGCTGGCCC GCCTCCTATC 
AGCAGATCGG GAGCCGCAAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATGAGCATT 
CAGGACAGCC TGGCGCTTCA ACCCAAGTTA ACTAGACACA AGGAGCTTGC ATCTGAAAAC 
ATCAATTTGA GAACTAGGAA GTACTCCAGC AGCAGCCTGA GTCACATGGG AGCCTACAGC 
CGATCACTTC CCAGGTTGTA CAGAGCCACA GAGAACCAGC TGACACCTCT CAGCTTGCCT 
CCAAGAAACT CTCTGGGCAA TTCCAAACGA ACAAAACTTG GGGAAAAGGA TCTACCTCAT 
AGCGTAATAG ACAATGACAA TTACCTTAAT TTTTCTTCTT TGAGCTCAGG GGCTTTACCC 
TATAAAACCT CTGCTTCTGA AGGCAATCCT TATGTAAGTT CTACCCTCAG TGTCCCTGCC 
AGTCCACGAG TGGCTCGGAA GATGCTTCTG GCCTCCACCT CCTCCTGTGC CTCTGATGAC 
TTTGATCAGG CTTCATATGT GGGGACAAAC CCGAGTCATT CACTTCTTGC TGGAGAGTCA 
GACAGAGTTT TTGCGACCAG GAGGAACTTC TCTTGTGGAT CTGTGGAATT TGATGAGGCA 
GATTTGGAAA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGCCCTTCG GGAACGGAAA 
AGCAGTATTA GCTCCATTTC AGGACGTGAT GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAAAGAC TCAGGGAGCA GGAAATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGTCTCT GTGCTGAATA CACAAAGCCT GACAGTCGCT TATCTACTGG GACCACCGTG 
GAAGATGTGC AGAAAATCAA CAAGGAGCTT GAGAAGCTGC AGCTCTCTGA TGAGGAGTCT 
GTGTTTGAGG AAGCCCTCAT GAGCCCTGAC ACAAGATACA GGTGCCACCG GAAAGACTCC 
CTCCCTGATG CAGACTTGGC AAGCTGTGGG AGTCTCAGTC AGAGCAGTGC CAGCTTCTTT 
ACCCCCAGGA GCACCAGGAA TGATGAACTA CTCAGTGACC TCACCCGGAC TCCTCCACCA 
CCATCCTCCA CCTTTCCGAA AGCTTCCAGC GAGTCCTCTT ATCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAGAGA TCTCAGGAGT TGGCTGCAAT GGAAGAAACC 
CGGATAGTCA TTCTGAACAA CCTCGAGGAA CTTAAGCAAA AAATCAAAGA CATAAATGAT 
CAGATGGATG AGTCTTTCAG AGAGTTGGAT ATGGAATGTG CTCTTTTGGA TGGAGAACAG 
AAATCTGAAA CAACTGAACT TATGAAGGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATAGCTGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGATGCTGA CCTGTTGGAT 
GTTGAAAGCA AACACTTTGA AGACCTGGAG TTCCAGCAGC TTGAACATGA GAGCCGTCTA 
GATGAAGAAA AGGAGAACTT GACTCAACAG CTCCTGCGTG AAGTTGCTGA ATATCAACGG 
AACATCGTTT CTAGAAAGGA AAAAATTTCT GCATTGAAAA AGCAAGCCAA TCACATTGTT 
CAGCAGGCTC AGAGAGAGCA AGATCATTTT GTGAAAGAAA AGAATAATTT AATAATGATG 
TTGCAAAGAG AAAAGGAGAA TCTTTGTAAT TTGGAAAAGA AATACTCCAG CCTCTCTGGG 
GGGAAAGGGT TTCCCGTTAA CCCCAATACT TTAAAAGAGG GCTATATCAG TGTAAATGAG 
ATTAATGAGC CGTGTGGCAA TTCCACGAAT CTATCCCCTT CCACTCAGTT TCCTGCTGAT 
GCTGATGCTG TTGCCACTGA GCCTGCCACA GCTGTGCTGG CGAGCCAGCC ACAGAGTAAA 
GAGCACTTTA GAAGTCTGGA AGAAAGGAAA AAACAGCATA AAGAAGGCCT CTATCTGAGT 
GATACTTTGC CTCGAAAGAA AACCACATCT TCCATCTCCC CACATTTCAG CAGTGCTACT 
ATGGGGAGAA GCATCACCCC AAAGGCCCAT CTGCCCCTAG GACAGAGTAA CAGCTGTGGA 
AGTGTGCTCC CTCCCTCACT GGCAGCCATG GCCAAAGACT CAGAATCTCG GAGGATGCTC 
AGAGGTTATA ATCACCAACA GATGAGTGAA GGACACAGGC AGAAATCTGA ATTTTATAAC 
CGCACAGCAT CTGAATCAAA TGTCTACTTG AATAGTTTCC ATTATCCAGA TCACAGCTAC 
AAGGACCAGG CCTTTGATAC TCTGAGCCTC GATAGCTCTG ATAGCATGGA GACCAGCATC 
TCTGCTTGCT CACCAGACAA CATCTCTAGT GCCAGCACTT CAAATATTGC TAGAATAGAA 
GAAATGGAGA GACTTTTGAA GCAGGCTCAT GCAGAAAAGA CGCGGCTGCT CGAATCCAGG 
GAACGGGAAA TGGAAGCCAA AAAACGAGCC CTGGAAGAAG AAAAACGACG CCGGGAAATC 
CTGGAAAAAC GATTACAGGA AGAAACTAGC CAGAGGCAGA AGTTAATAGA AAAGGAAGTA 
AAAATAAGGG AGAGACAAAG GGCACAGGCT CGTCCTTTGA CACGCTACCT GCCTGTCCGG 
AAGGAAGACT TTGATTTGCG GAGCCATGTA GAGACTGCTG GCCACAATAT TGACACCTGT 
TACCATGTAT CAATCACAGA GAAGACCTGC CGAGGATTCC TCATCAAAAT GGGTGGGAAA 
ATTAAAACGT GGAAAAAACG TTGGTTTGTT TTTGATCGGA ACAAGCGAAC ATTCTCTTAT 
TATGCAGACA AGCATGAAAC TAAATTGAAA GGAGTAATAT ACTTTCAAGC CATTGAAGAA 
GTCTATTATG ATCACCTCAA GAATGCTAAT AAGAGTCCTA ATCCGTTACT CACCTTTAGC 
GTCAAGACTC ATGACAGAAT CTATTATATG GTAGCCCCAT CGCCAGAAGC CATGCGGATC 
TGGATGGATG TTATAGTTAC GGGGGCAGAA GGTTACACTC ACTTCTTGTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

p53 target miR-29c-3p suppresses colon cancer cell invasion and migration through inhibition of PHLDB2.
Biochemical and biophysical research communications487(1)90-95(2017 05)
Chen G,Zhou T,Li Y,Yu Z,Sun L

Prickle1 promotes focal adhesion disassembly in cooperation with the CLASP-LL5? complex in migrating cells.
Journal of cell science129(16)3115-29(2016 08)
Lim BC,Matsumoto S,Yamamoto H,Mizuno H,Kikuta J,Ishii M,Kikuchi A

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
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PHLDB2 cDNA ORF clone, Homo sapiens(human)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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