PHLDB2 cDNA ORF clone, Bos taurus(cattle)

The following PHLDB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHLDB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa37104 NM_001206308.1
Latest version!
Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa15595 XM_005201281.4
Latest version!
Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa15599 XM_015471046.2
Latest version!
Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa15601 XM_015471052.2
Latest version!
Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa218690 XM_024977109.1
Latest version!
Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa218691 XM_024977397.1
Latest version!
Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa47504 XM_024977359.1
Latest version!
Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa87685 XM_015471035.2
Latest version!
Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa87685 XM_015471036.1
Latest version!
Bos taurus pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa87686 XM_015471038.2
Latest version!
Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa87686 XM_015471041.1
Latest version!
Bos taurus pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa87686 XM_015471039.2
Latest version!
Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa87687 XM_015471042.2
Latest version!
Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa87688 XM_015471043.2
Latest version!
Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa87689 XM_015471050.2
Latest version!
Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa87690 XM_015471053.2
Latest version!
Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
View Old Accession Versions >>
OBa87686 XM_015471038.1 Bos taurus pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa15595 XM_005201281.3 Bos taurus pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa87689 XM_015471050.1 Bos taurus pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa87690 XM_015471053.1 Bos taurus pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa87686 XM_015471039.1 Bos taurus pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa87688 XM_015471043.1 Bos taurus pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa87685 XM_015471035.1 Bos taurus pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa87687 XM_015471042.1 Bos taurus pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa15599 XM_015471046.1 Bos taurus pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa15601 XM_015471052.1 Bos taurus pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa37104
    Clone ID Related Accession (Same CDS sequence) NM_001206308.1
    Accession Version NM_001206308.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3765bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000001.1. On Apr 20, 2011 this sequence version replaced XM_874620.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR5571260.43210.1, SRR5571260.42605.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAGAGC AGAGCCACAT GCAAAAGCAG CTGGATTTAC AGAATGGTAG CTTAGAGGCA 
    GGCTTTGCGG AGAACTCCCT CGAAAACGAT TCCCAAAACA TGATGGAGAG CCTGAGCCCA
    AAGAAATATT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTGTTCTGG CTCCTATTTA
    ACCCTCTCAC AACCTGTGCC TGCTAAAAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA
    AGTAGCCCTT CTTTGGCCAA AATTCAGGGA ACGAAGCAGT TCTCTTGTGA TGGAAGTGAG
    AAAAGTATTT CCATGAAACC GCCCACCCCT TTACGCAGCA CCTCACCCTC CCTTAGTGGA
    TATGCACTTG GGAGAGCAGA CTTTGATCAT TTTCTTGGCC GGGACGCCGA AAGGTCCTTG
    AGGCTTTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGAATAAATC CCATGACAAT
    GTCTACTTTC TCGGAGGGCT GGAAGGCCGA AAAGCATCTG GCTCGCTCCT GACCATGTGG
    AATGGAAGTT CCCTGAGTGA CACTGGCTCC TCACCCATCA ACAGGTCAGG GGCGGCCAGC
    ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATAAGCATCC AGGACAACCT GACACTTCAG
    CCCAAGTTGA GCAGACACCG GGACCCGGCA TCGGAAAACA TCAGTTTAAG AACCAGGAAG
    TACTCTAGCG GCAGCCTGAG TCACATGGGC GCCTACAGCC GCTCTCTCCC CAGGTTGCAT
    AGAGCCACCG AGAACCAGTT GGCACCCCTC AGTCTGCCTC CAAGAAGCTC TCTGGGCAAT
    TCCAAACGAA CGAAACTGGG GGAAAAGGAT CTACCTCATT GCATTATAGA CAATGACAAT
    TACCTGAATT TTTCTTCTTT GAGCTCAGGA GCTTTGCCCT ATAAAGCCTC TGCCTCCGAG
    GGCAGTGCGT ACGTGAGTTC CACCCTCAGC GTGCCTGCCA GTCCTCGAGT GGCTCGCAAG
    ATGCTTCTGG CCTCCACCTC CTCCTGCACC TCTGACGACC TTGACAGGGC TTCCTACTCG
    GGTACCAGCC CTGGTCATTC GTTTCCTCCT GGGGAGCTGG ACCGAGCATT TGCAGCCAGA
    AGGAACTTCT CGTGTGGATC TGTCGAGTTT GATGATGCAG ACTTGGACAG CCTCAGACAG
    GCCTCAGGAA CCCCCCAGCC TGTCCTGCGG GAACGGAAAA GCAGCATTAG CTCCATTTCC
    GGACGAGACG ACCTGATGGA TTATCATCGG CGGCAGAGGG AGGAGAGACT CAGGGAGCAG
    GAGATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGCCTCTG TGCTGAGTAC
    ACAAAGCCTG ACAGTCGTGT GTCCCCCGGC ACCACCGTGG CAGATGTGCA GAAAATCAAT
    AAAGAGCTGG AGAAACTGCA GCTCTCGGAT GAGGAGTCTG TGTTTGAAGA AGCCCTGGTG
    AGCCCGGACA CCAGGTACAG GTGCCACCAG AAAAGCGCTC TCCACGACGC GGACCTGGCA
    GGCTTTGGGA GCCTCAGTCA GAGCAGTGCC AGCTTCCTCC CGCCACGGGG CGCCAGGAAT
    GATGAACTTC TCGGGGACCT CGCCAGGACT CCCCCGCCGC CCTCCTCTGC CTTTCTGAAA
    GCTTCCGGCG AGTCCGCTTA CCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA
    GAACAAAGGA TTCAGGAGTT GACTGCGATG GAAGAGACCC GGATAGCAAT TCTGAACAAC
    CTTGAGGAAC TTGAACAAAA AATCAAAGAT ATAAATGACC AGATGGAGGA ATCTTCCAGA
    GAGCTGGATA TGGAATGTGC TCTCTTGGAT GGAGAACAAA AATCAGAAAC AACTGAACTT
    ATGAAAGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TCACAGAACT GGAAAAGAAC
    ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG
    AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG
    AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCCGACC TACTGGATGT TGAGAGCAAA
    CACTTTGAAG ACCTGGAATT CCAGCAGCTT GAGCGTGAAA GCCGTCTAGA TGAGGAAAAG
    GAGAACCTGA CCCAGCAACT CCTGCGTGAA GTGGCTGAGT ATCAACGGAA CATTGTTACT
    AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCTAATC ACATTGTCCA GCAGGCTCAA
    AGAGAACAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGCT GCAGAGAGAA
    AAGGAAAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT
    CCTGTCACCC CCAATACTCT GAAAGAGGGC TATATCATTG TAAATGAAAT TAATGAGTCA
    TGTGGCAGTT CCACGAATCT CCCCCCTTCC ACTCAGTTCC CTGCTGATGC TGATGCTACT
    GCCGCTGGGC CTACCACAGC TGTGCTGATG AGCCAGCCAC AAAGTAAAGA GCATTTTAGA
    AGTCTGGAAG AAAGGAAAAA ACAGCACCAG GAAGGCCTCT ATCTGAGTGA CACTTTGCCT
    CGAAAGAAAA CCACACCTTC CATATCCCCA CATTTCAGCA GTGCTACTAT GGGGAGAAGC
    ACCACCCCAA AGGGCCACCT GCCCCTGGGA CAGAGCAACA GCTGTGGCAG CGTGCTCCCC
    CACTCGCTAG CAACCATGAC CAAAGACTCA GAATCTCGGA GGATGCTCAG AGGATATAAT
    CACCAACAGA TGAGTGAAGG ACAAAGGCAG AAGTCTTCTG AATTTTACAA CCGCACAGCA
    TCTGAATCGA ATGTCTACTT GAACAGTTTC CACTACCCAG ATCACAGCTA CAAGGACCAG
    GCCTTTGATA CTCTGAGCCT AGACAGCTCT GACAGCATGG AGACCAGCAT CTCTGCCTGC
    TCACCTGACA ATATCTCTAG TGCCAGCACT TCAAATATTG CCCGAATAGA GGAAATGGAG
    AGACTGTTGA AGCAGGCTCA TGCAGAGAAG ACAAGGCTGC TTGAATCCAG GGAACGAGAA
    ATGGAAGCCA AGAAACGCGC TCTGGAAGAA GAAAAACGAC GTCGAGAACT CCTGGAAAAA
    CGGTTGCAAG AAGAAACTAG CCAGAGGCAG AAGTTAATTG AAAAGGAAGT AAAAATAAGG
    GAGAAACAAA GGGCACAGGC TCGACCTCTG ACCCGCTATC TGCCTGTGCG GAAGGAAGAC
    TTTGATTTGC GGAGCCACGT AGAGACTGCT GGCCACAATA TCGATACGTG TTATCATGTG
    TCAATCACAG AGAAGACCTG CAGAGGATTC CTCATCAAAA TGGGTGGAAA AATTAAAACC
    TGGAAAAAAC GTTGGTTTGT TTTTGATCGG AACAAGCGCA CCTTCTCTTA TTATGCAGAC
    AAGCATGAAG CTAAATTAAA AGGAGTAATA TACTTTCAAG CCATTGAAGA AGTATATTAT
    GATCACCTCA AGAACGCTAA TAAGAGTCCT AATCCTTTAC TTACCTTTAG CGTCAAGACA
    CATGACAGAA TCTACTACAT GGTGGCCCCG TCGCCAGAAG CCATGCGGAT CTGGATGGAC
    GTTATCGTGA CTGGGGCAGA AGGCTACACT CACTTCTTGT TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193237.1
    CDS1..3765
    Translation

    Target ORF information:

    RefSeq Version NM_001206308.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206308.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAGAGC AGAGCCACAT GCAAAAGCAG CTGGATTTAC AGAATGGTAG CTTAGAGGCA 
    GGCTTTGCGG AGAACTCCCT CGAAAACGAT TCCCAAAACA TGATGGAGAG CCTGAGCCCA
    AAGAAATATT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTGTTCTGG CTCCTATTTA
    ACCCTCTCAC AACCTGTGCC TGCTAAAAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA
    AGTAGCCCTT CTTTGGCCAA AATTCAGGGA ACGAAGCAGT TCTCTTGTGA TGGAAGTGAG
    AAAAGTATTT CCATGAAACC GCCCACCCCT TTACGCAGCA CCTCACCCTC CCTTAGTGGA
    TATGCACTTG GGAGAGCAGA CTTTGATCAT TTTCTTGGCC GGGACGCCGA AAGGTCCTTG
    AGGCTTTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGAATAAATC CCATGACAAT
    GTCTACTTTC TCGGAGGGCT GGAAGGCCGA AAAGCATCTG GCTCGCTCCT GACCATGTGG
    AATGGAAGTT CCCTGAGTGA CACTGGCTCC TCACCCATCA ACAGGTCAGG GGCGGCCAGC
    ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATAAGCATCC AGGACAACCT GACACTTCAG
    CCCAAGTTGA GCAGACACCG GGACCCGGCA TCGGAAAACA TCAGTTTAAG AACCAGGAAG
    TACTCTAGCG GCAGCCTGAG TCACATGGGC GCCTACAGCC GCTCTCTCCC CAGGTTGCAT
    AGAGCCACCG AGAACCAGTT GGCACCCCTC AGTCTGCCTC CAAGAAGCTC TCTGGGCAAT
    TCCAAACGAA CGAAACTGGG GGAAAAGGAT CTACCTCATT GCATTATAGA CAATGACAAT
    TACCTGAATT TTTCTTCTTT GAGCTCAGGA GCTTTGCCCT ATAAAGCCTC TGCCTCCGAG
    GGCAGTGCGT ACGTGAGTTC CACCCTCAGC GTGCCTGCCA GTCCTCGAGT GGCTCGCAAG
    ATGCTTCTGG CCTCCACCTC CTCCTGCACC TCTGACGACC TTGACAGGGC TTCCTACTCG
    GGTACCAGCC CTGGTCATTC GTTTCCTCCT GGGGAGCTGG ACCGAGCATT TGCAGCCAGA
    AGGAACTTCT CGTGTGGATC TGTCGAGTTT GATGATGCAG ACTTGGACAG CCTCAGACAG
    GCCTCAGGAA CCCCCCAGCC TGTCCTGCGG GAACGGAAAA GCAGCATTAG CTCCATTTCC
    GGACGAGACG ACCTGATGGA TTATCATCGG CGGCAGAGGG AGGAGAGACT CAGGGAGCAG
    GAGATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGCCTCTG TGCTGAGTAC
    ACAAAGCCTG ACAGTCGTGT GTCCCCCGGC ACCACCGTGG CAGATGTGCA GAAAATCAAT
    AAAGAGCTGG AGAAACTGCA GCTCTCGGAT GAGGAGTCTG TGTTTGAAGA AGCCCTGGTG
    AGCCCGGACA CCAGGTACAG GTGCCACCAG AAAAGCGCTC TCCACGACGC GGACCTGGCA
    GGCTTTGGGA GCCTCAGTCA GAGCAGTGCC AGCTTCCTCC CGCCACGGGG CGCCAGGAAT
    GATGAACTTC TCGGGGACCT CGCCAGGACT CCCCCGCCGC CCTCCTCTGC CTTTCTGAAA
    GCTTCCGGCG AGTCCGCTTA CCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA
    GAACAAAGGA TTCAGGAGTT GACTGCGATG GAAGAGACCC GGATAGCAAT TCTGAACAAC
    CTTGAGGAAC TTGAACAAAA AATCAAAGAT ATAAATGACC AGATGGAGGA ATCTTCCAGA
    GAGCTGGATA TGGAATGTGC TCTCTTGGAT GGAGAACAAA AATCAGAAAC AACTGAACTT
    ATGAAAGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TCACAGAACT GGAAAAGAAC
    ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG
    AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG
    AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCCGACC TACTGGATGT TGAGAGCAAA
    CACTTTGAAG ACCTGGAATT CCAGCAGCTT GAGCGTGAAA GCCGTCTAGA TGAGGAAAAG
    GAGAACCTGA CCCAGCAACT CCTGCGTGAA GTGGCTGAGT ATCAACGGAA CATTGTTACT
    AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCTAATC ACATTGTCCA GCAGGCTCAA
    AGAGAACAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGCT GCAGAGAGAA
    AAGGAAAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT
    CCTGTCACCC CCAATACTCT GAAAGAGGGC TATATCATTG TAAATGAAAT TAATGAGTCA
    TGTGGCAGTT CCACGAATCT CCCCCCTTCC ACTCAGTTCC CTGCTGATGC TGATGCTACT
    GCCGCTGGGC CTACCACAGC TGTGCTGATG AGCCAGCCAC AAAGTAAAGA GCATTTTAGA
    AGTCTGGAAG AAAGGAAAAA ACAGCACCAG GAAGGCCTCT ATCTGAGTGA CACTTTGCCT
    CGAAAGAAAA CCACACCTTC CATATCCCCA CATTTCAGCA GTGCTACTAT GGGGAGAAGC
    ACCACCCCAA AGGGCCACCT GCCCCTGGGA CAGAGCAACA GCTGTGGCAG CGTGCTCCCC
    CACTCGCTAG CAACCATGAC CAAAGACTCA GAATCTCGGA GGATGCTCAG AGGATATAAT
    CACCAACAGA TGAGTGAAGG ACAAAGGCAG AAGTCTTCTG AATTTTACAA CCGCACAGCA
    TCTGAATCGA ATGTCTACTT GAACAGTTTC CACTACCCAG ATCACAGCTA CAAGGACCAG
    GCCTTTGATA CTCTGAGCCT AGACAGCTCT GACAGCATGG AGACCAGCAT CTCTGCCTGC
    TCACCTGACA ATATCTCTAG TGCCAGCACT TCAAATATTG CCCGAATAGA GGAAATGGAG
    AGACTGTTGA AGCAGGCTCA TGCAGAGAAG ACAAGGCTGC TTGAATCCAG GGAACGAGAA
    ATGGAAGCCA AGAAACGCGC TCTGGAAGAA GAAAAACGAC GTCGAGAACT CCTGGAAAAA
    CGGTTGCAAG AAGAAACTAG CCAGAGGCAG AAGTTAATTG AAAAGGAAGT AAAAATAAGG
    GAGAAACAAA GGGCACAGGC TCGACCTCTG ACCCGCTATC TGCCTGTGCG GAAGGAAGAC
    TTTGATTTGC GGAGCCACGT AGAGACTGCT GGCCACAATA TCGATACGTG TTATCATGTG
    TCAATCACAG AGAAGACCTG CAGAGGATTC CTCATCAAAA TGGGTGGAAA AATTAAAACC
    TGGAAAAAAC GTTGGTTTGT TTTTGATCGG AACAAGCGCA CCTTCTCTTA TTATGCAGAC
    AAGCATGAAG CTAAATTAAA AGGAGTAATA TACTTTCAAG CCATTGAAGA AGTATATTAT
    GATCACCTCA AGAACGCTAA TAAGAGTCCT AATCCTTTAC TTACCTTTAG CGTCAAGACA
    CATGACAGAA TCTACTACAT GGTGGCCCCG TCGCCAGAAG CCATGCGGAT CTGGATGGAC
    GTTATCGTGA CTGGGGCAGA AGGCTACACT CACTTCTTGT TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15595
    Clone ID Related Accession (Same CDS sequence) XM_005201281.4 , XM_005201281.3
    Accession Version XM_005201281.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4032bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201281.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT
    GTAAATGAAA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTC
    CCTGCTGATG CTGATGCTAC TGCCGCTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA
    CAAAGTAAAG AGCAAAGATC GTCTGTCTGT CCTGGATTTA TGTTTCCTTC CACTCTTTCT
    TCTCATCCTA TCACTCAACC TCCATCAGCC CCTTGGCCTG AAGCCGTGGC TACCTTTGTC
    GATTCTTTTC CTTTAAATGA CACACCTCCT CCTCTACCAG CTAAGAAACA CAGGAGGCAA
    CAGCAGCAGG ACCAGCAGCA TTTTAGAAGT CTGGAAGAAA GGAAAAAACA GCACCAGGAA
    GGCCTCTATC TGAGTGACAC TTTGCCTCGA AAGAAAACCA CACCTTCCAT ATCCCCACAT
    TTCAGCAGTG CTACTATGGG GAGAAGCACC ACCCCAAAGG GCCACCTGCC CCTGGGACAG
    AGCAACAGCT GTGGCAGCGT GCTCCCCCAC TCGCTAGCAA CCATGACCAA AGACTCAGAA
    TCTCGGAGGA TGCTCAGAGG ATATAATCAC CAACAGATGA GTGAAGGACA AAGGCAGAAG
    TCTTCTGAAT TTTACAACCG CACAGCATCT GAATCGAATG TCTACTTGAA CAGTTTCCAC
    TACCCAGATC ACAGCTACAA GGACCAGGCC TTTGATACTC TGAGCCTAGA CAGCTCTGAC
    AGCATGGAGA CCAGCATCTC TGCCTGCTCA CCTGACAATA TCTCTAGTGC CAGCACTTCA
    AATATTGCCC GAATAGAGGA AATGGAGAGA CTGTTGAAGC AGGCTCATGC AGAGAAGACA
    AGGCTGCTTG AATCCAGGGA ACGAGAAATG GAAGCCAAGA AACGCGCTCT GGAAGAAGAA
    AAACGACGTC GAGAACTCCT GGAAAAACGG TTGCAAGAAG AAACTAGCCA GAGGCAGAAG
    TTAATTGAAA AGGAAGTAAA AATAAGGGAG AAACAAAGGG CACAGGCTCG ACCTCTGACC
    CGCTATCTGC CTGTGCGGAA GGAAGACTTT GATTTGCGGA GCCACGTAGA GACTGCTGGC
    CACAATATCG ATACGTGTTA TCATGTGTCA ATCACAGAGA AGACCTGCAG AGGATTCCTC
    ATCAAAATGG GTGGAAAAAT TAAAACCTGG AAAAAACGTT GGTTTGTTTT TGATCGGAAC
    AAGCGCACCT TCTCTTATTA TGCAGACAAG CATGAAGCTA AATTAAAAGG AGTAATATAC
    TTTCAAGCCA TTGAAGAAGT ATATTATGAT CACCTCAAGA ACGCTAATAA GAGTCCTAAT
    CCTTTACTTA CCTTTAGCGT CAAGACACAT GACAGAATCT ACTACATGGT GGCCCCGTCG
    CCAGAAGCCA TGCGGATCTG GATGGACGTT ATCGTGACTG GGGCAGAAGG CTACACTCAC
    TTCTTGTTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201338.1
    CDS283..4314
    Translation

    Target ORF information:

    RefSeq Version XM_005201281.4
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201281.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT
    GTAAATGAAA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTC
    CCTGCTGATG CTGATGCTAC TGCCGCTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA
    CAAAGTAAAG AGCAAAGATC GTCTGTCTGT CCTGGATTTA TGTTTCCTTC CACTCTTTCT
    TCTCATCCTA TCACTCAACC TCCATCAGCC CCTTGGCCTG AAGCCGTGGC TACCTTTGTC
    GATTCTTTTC CTTTAAATGA CACACCTCCT CCTCTACCAG CTAAGAAACA CAGGAGGCAA
    CAGCAGCAGG ACCAGCAGCA TTTTAGAAGT CTGGAAGAAA GGAAAAAACA GCACCAGGAA
    GGCCTCTATC TGAGTGACAC TTTGCCTCGA AAGAAAACCA CACCTTCCAT ATCCCCACAT
    TTCAGCAGTG CTACTATGGG GAGAAGCACC ACCCCAAAGG GCCACCTGCC CCTGGGACAG
    AGCAACAGCT GTGGCAGCGT GCTCCCCCAC TCGCTAGCAA CCATGACCAA AGACTCAGAA
    TCTCGGAGGA TGCTCAGAGG ATATAATCAC CAACAGATGA GTGAAGGACA AAGGCAGAAG
    TCTTCTGAAT TTTACAACCG CACAGCATCT GAATCGAATG TCTACTTGAA CAGTTTCCAC
    TACCCAGATC ACAGCTACAA GGACCAGGCC TTTGATACTC TGAGCCTAGA CAGCTCTGAC
    AGCATGGAGA CCAGCATCTC TGCCTGCTCA CCTGACAATA TCTCTAGTGC CAGCACTTCA
    AATATTGCCC GAATAGAGGA AATGGAGAGA CTGTTGAAGC AGGCTCATGC AGAGAAGACA
    AGGCTGCTTG AATCCAGGGA ACGAGAAATG GAAGCCAAGA AACGCGCTCT GGAAGAAGAA
    AAACGACGTC GAGAACTCCT GGAAAAACGG TTGCAAGAAG AAACTAGCCA GAGGCAGAAG
    TTAATTGAAA AGGAAGTAAA AATAAGGGAG AAACAAAGGG CACAGGCTCG ACCTCTGACC
    CGCTATCTGC CTGTGCGGAA GGAAGACTTT GATTTGCGGA GCCACGTAGA GACTGCTGGC
    CACAATATCG ATACGTGTTA TCATGTGTCA ATCACAGAGA AGACCTGCAG AGGATTCCTC
    ATCAAAATGG GTGGAAAAAT TAAAACCTGG AAAAAACGTT GGTTTGTTTT TGATCGGAAC
    AAGCGCACCT TCTCTTATTA TGCAGACAAG CATGAAGCTA AATTAAAAGG AGTAATATAC
    TTTCAAGCCA TTGAAGAAGT ATATTATGAT CACCTCAAGA ACGCTAATAA GAGTCCTAAT
    CCTTTACTTA CCTTTAGCGT CAAGACACAT GACAGAATCT ACTACATGGT GGCCCCGTCG
    CCAGAAGCCA TGCGGATCTG GATGGACGTT ATCGTGACTG GGGCAGAAGG CTACACTCAC
    TTCTTGTTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15595
    Clone ID Related Accession (Same CDS sequence) XM_005201281.4 , XM_005201281.3
    Accession Version XM_005201281.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4032bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103810.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201281.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT
    GTAAATGAAA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTC
    CCTGCTGATG CTGATGCTAC TGCCGCTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA
    CAAAGTAAAG AGCAAAGATC GTCTGTCTGT CCTGGATTTA TGTTTCCTTC CACTCTTTCT
    TCTCATCCTA TCACTCAACC TCCATCAGCC CCTTGGCCTG AAGCCGTGGC TACCTTTGTC
    GATTCTTTTC CTTTAAATGA CACACCTCCT CCTCTACCAG CTAAGAAACA CAGGAGGCAA
    CAGCAGCAGG ACCAGCAGCA TTTTAGAAGT CTGGAAGAAA GGAAAAAACA GCACCAGGAA
    GGCCTCTATC TGAGTGACAC TTTGCCTCGA AAGAAAACCA CACCTTCCAT ATCCCCACAT
    TTCAGCAGTG CTACTATGGG GAGAAGCACC ACCCCAAAGG GCCACCTGCC CCTGGGACAG
    AGCAACAGCT GTGGCAGCGT GCTCCCCCAC TCGCTAGCAA CCATGACCAA AGACTCAGAA
    TCTCGGAGGA TGCTCAGAGG ATATAATCAC CAACAGATGA GTGAAGGACA AAGGCAGAAG
    TCTTCTGAAT TTTACAACCG CACAGCATCT GAATCGAATG TCTACTTGAA CAGTTTCCAC
    TACCCAGATC ACAGCTACAA GGACCAGGCC TTTGATACTC TGAGCCTAGA CAGCTCTGAC
    AGCATGGAGA CCAGCATCTC TGCCTGCTCA CCTGACAATA TCTCTAGTGC CAGCACTTCA
    AATATTGCCC GAATAGAGGA AATGGAGAGA CTGTTGAAGC AGGCTCATGC AGAGAAGACA
    AGGCTGCTTG AATCCAGGGA ACGAGAAATG GAAGCCAAGA AACGCGCTCT GGAAGAAGAA
    AAACGACGTC GAGAACTCCT GGAAAAACGG TTGCAAGAAG AAACTAGCCA GAGGCAGAAG
    TTAATTGAAA AGGAAGTAAA AATAAGGGAG AAACAAAGGG CACAGGCTCG ACCTCTGACC
    CGCTATCTGC CTGTGCGGAA GGAAGACTTT GATTTGCGGA GCCACGTAGA GACTGCTGGC
    CACAATATCG ATACGTGTTA TCATGTGTCA ATCACAGAGA AGACCTGCAG AGGATTCCTC
    ATCAAAATGG GTGGAAAAAT TAAAACCTGG AAAAAACGTT GGTTTGTTTT TGATCGGAAC
    AAGCGCACCT TCTCTTATTA TGCAGACAAG CATGAAGCTA AATTAAAAGG AGTAATATAC
    TTTCAAGCCA TTGAAGAAGT ATATTATGAT CACCTCAAGA ACGCTAATAA GAGTCCTAAT
    CCTTTACTTA CCTTTAGCGT CAAGACACAT GACAGAATCT ACTACATGGT GGCCCCGTCG
    CCAGAAGCCA TGCGGATCTG GATGGACGTT ATCGTGACTG GGGCAGAAGG CTACACTCAC
    TTCTTGTTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201338.1
    CDS328..4359
    Translation

    Target ORF information:

    RefSeq Version XM_005201281.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201281.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT
    GTAAATGAAA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTC
    CCTGCTGATG CTGATGCTAC TGCCGCTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA
    CAAAGTAAAG AGCAAAGATC GTCTGTCTGT CCTGGATTTA TGTTTCCTTC CACTCTTTCT
    TCTCATCCTA TCACTCAACC TCCATCAGCC CCTTGGCCTG AAGCCGTGGC TACCTTTGTC
    GATTCTTTTC CTTTAAATGA CACACCTCCT CCTCTACCAG CTAAGAAACA CAGGAGGCAA
    CAGCAGCAGG ACCAGCAGCA TTTTAGAAGT CTGGAAGAAA GGAAAAAACA GCACCAGGAA
    GGCCTCTATC TGAGTGACAC TTTGCCTCGA AAGAAAACCA CACCTTCCAT ATCCCCACAT
    TTCAGCAGTG CTACTATGGG GAGAAGCACC ACCCCAAAGG GCCACCTGCC CCTGGGACAG
    AGCAACAGCT GTGGCAGCGT GCTCCCCCAC TCGCTAGCAA CCATGACCAA AGACTCAGAA
    TCTCGGAGGA TGCTCAGAGG ATATAATCAC CAACAGATGA GTGAAGGACA AAGGCAGAAG
    TCTTCTGAAT TTTACAACCG CACAGCATCT GAATCGAATG TCTACTTGAA CAGTTTCCAC
    TACCCAGATC ACAGCTACAA GGACCAGGCC TTTGATACTC TGAGCCTAGA CAGCTCTGAC
    AGCATGGAGA CCAGCATCTC TGCCTGCTCA CCTGACAATA TCTCTAGTGC CAGCACTTCA
    AATATTGCCC GAATAGAGGA AATGGAGAGA CTGTTGAAGC AGGCTCATGC AGAGAAGACA
    AGGCTGCTTG AATCCAGGGA ACGAGAAATG GAAGCCAAGA AACGCGCTCT GGAAGAAGAA
    AAACGACGTC GAGAACTCCT GGAAAAACGG TTGCAAGAAG AAACTAGCCA GAGGCAGAAG
    TTAATTGAAA AGGAAGTAAA AATAAGGGAG AAACAAAGGG CACAGGCTCG ACCTCTGACC
    CGCTATCTGC CTGTGCGGAA GGAAGACTTT GATTTGCGGA GCCACGTAGA GACTGCTGGC
    CACAATATCG ATACGTGTTA TCATGTGTCA ATCACAGAGA AGACCTGCAG AGGATTCCTC
    ATCAAAATGG GTGGAAAAAT TAAAACCTGG AAAAAACGTT GGTTTGTTTT TGATCGGAAC
    AAGCGCACCT TCTCTTATTA TGCAGACAAG CATGAAGCTA AATTAAAAGG AGTAATATAC
    TTTCAAGCCA TTGAAGAAGT ATATTATGAT CACCTCAAGA ACGCTAATAA GAGTCCTAAT
    CCTTTACTTA CCTTTAGCGT CAAGACACAT GACAGAATCT ACTACATGGT GGCCCCGTCG
    CCAGAAGCCA TGCGGATCTG GATGGACGTT ATCGTGACTG GGGCAGAAGG CTACACTCAC
    TTCTTGTTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15599
    Clone ID Related Accession (Same CDS sequence) XM_015471046.1 , XM_015471046.2
    Accession Version XM_015471046.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3846bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103810.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT
    GTAAATGAAA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTC
    CCTGCTGATG CTGATGCTAC TGCCGCTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA
    CAAAGTAAAG AGCATTTTAG AAGTCTGGAA GAAAGGAAAA AACAGCACCA GGAAGGCCTC
    TATCTGAGTG ACACTTTGCC TCGAAAGAAA ACCACACCTT CCATATCCCC ACATTTCAGC
    AGTGCTACTA TGGGGAGAAG CACCACCCCA AAGGGCCACC TGCCCCTGGG ACAGAGCAAC
    AGCTGTGGCA GCGTGCTCCC CCACTCGCTA GCAACCATGA CCAAAGACTC AGAATCTCGG
    AGGATGCTCA GAGGATATAA TCACCAACAG ATGAGTGAAG GACAAAGGCA GAAGTCTTCT
    GAATTTTACA ACCGCACAGC ATCTGAATCG AATGTCTACT TGAACAGTTT CCACTACCCA
    GATCACAGCT ACAAGGACCA GGCCTTTGAT ACTCTGAGCC TAGACAGCTC TGACAGCATG
    GAGACCAGCA TCTCTGCCTG CTCACCTGAC AATATCTCTA GTGCCAGCAC TTCAAATATT
    GCCCGAATAG AGGAAATGGA GAGACTGTTG AAGCAGGCTC ATGCAGAGAA GACAAGGCTG
    CTTGAATCCA GGGAACGAGA AATGGAAGCC AAGAAACGCG CTCTGGAAGA AGAAAAACGA
    CGTCGAGAAC TCCTGGAAAA ACGGTTGCAA GAAGAAACTA GCCAGAGGCA GAAGTTAATT
    GAAAAGGAAG TAAAAATAAG GGAGAAACAA AGGGCACAGG CTCGACCTCT GACCCGCTAT
    CTGCCTGTGC GGAAGGAAGA CTTTGATTTG CGGAGCCACG TAGAGACTGC TGGCCACAAT
    ATCGATACGT GTTATCATGT GTCAATCACA GAGAAGACCT GCAGAGGATT CCTCATCAAA
    ATGGGTGGAA AAATTAAAAC CTGGAAAAAA CGTTGGTTTG TTTTTGATCG GAACAAGCGC
    ACCTTCTCTT ATTATGCAGA CAAGCATGAA GCTAAATTAA AAGGAGTAAT ATACTTTCAA
    GCCATTGAAG AAGTATATTA TGATCACCTC AAGAACGCTA ATAAGAGTCC TAATCCTTTA
    CTTACCTTTA GCGTCAAGAC ACATGACAGA ATCTACTACA TGGTGGCCCC GTCGCCAGAA
    GCCATGCGGA TCTGGATGGA CGTTATCGTG ACTGGGGCAG AAGGCTACAC TCACTTCTTG
    TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326532.1
    CDS325..4170
    Translation

    Target ORF information:

    RefSeq Version XM_015471046.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471046.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT
    GTAAATGAAA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTC
    CCTGCTGATG CTGATGCTAC TGCCGCTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA
    CAAAGTAAAG AGCATTTTAG AAGTCTGGAA GAAAGGAAAA AACAGCACCA GGAAGGCCTC
    TATCTGAGTG ACACTTTGCC TCGAAAGAAA ACCACACCTT CCATATCCCC ACATTTCAGC
    AGTGCTACTA TGGGGAGAAG CACCACCCCA AAGGGCCACC TGCCCCTGGG ACAGAGCAAC
    AGCTGTGGCA GCGTGCTCCC CCACTCGCTA GCAACCATGA CCAAAGACTC AGAATCTCGG
    AGGATGCTCA GAGGATATAA TCACCAACAG ATGAGTGAAG GACAAAGGCA GAAGTCTTCT
    GAATTTTACA ACCGCACAGC ATCTGAATCG AATGTCTACT TGAACAGTTT CCACTACCCA
    GATCACAGCT ACAAGGACCA GGCCTTTGAT ACTCTGAGCC TAGACAGCTC TGACAGCATG
    GAGACCAGCA TCTCTGCCTG CTCACCTGAC AATATCTCTA GTGCCAGCAC TTCAAATATT
    GCCCGAATAG AGGAAATGGA GAGACTGTTG AAGCAGGCTC ATGCAGAGAA GACAAGGCTG
    CTTGAATCCA GGGAACGAGA AATGGAAGCC AAGAAACGCG CTCTGGAAGA AGAAAAACGA
    CGTCGAGAAC TCCTGGAAAA ACGGTTGCAA GAAGAAACTA GCCAGAGGCA GAAGTTAATT
    GAAAAGGAAG TAAAAATAAG GGAGAAACAA AGGGCACAGG CTCGACCTCT GACCCGCTAT
    CTGCCTGTGC GGAAGGAAGA CTTTGATTTG CGGAGCCACG TAGAGACTGC TGGCCACAAT
    ATCGATACGT GTTATCATGT GTCAATCACA GAGAAGACCT GCAGAGGATT CCTCATCAAA
    ATGGGTGGAA AAATTAAAAC CTGGAAAAAA CGTTGGTTTG TTTTTGATCG GAACAAGCGC
    ACCTTCTCTT ATTATGCAGA CAAGCATGAA GCTAAATTAA AAGGAGTAAT ATACTTTCAA
    GCCATTGAAG AAGTATATTA TGATCACCTC AAGAACGCTA ATAAGAGTCC TAATCCTTTA
    CTTACCTTTA GCGTCAAGAC ACATGACAGA ATCTACTACA TGGTGGCCCC GTCGCCAGAA
    GCCATGCGGA TCTGGATGGA CGTTATCGTG ACTGGGGCAG AAGGCTACAC TCACTTCTTG
    TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15599
    Clone ID Related Accession (Same CDS sequence) XM_015471046.1 , XM_015471046.2
    Accession Version XM_015471046.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3846bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471046.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT
    GTAAATGAAA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTC
    CCTGCTGATG CTGATGCTAC TGCCGCTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA
    CAAAGTAAAG AGCATTTTAG AAGTCTGGAA GAAAGGAAAA AACAGCACCA GGAAGGCCTC
    TATCTGAGTG ACACTTTGCC TCGAAAGAAA ACCACACCTT CCATATCCCC ACATTTCAGC
    AGTGCTACTA TGGGGAGAAG CACCACCCCA AAGGGCCACC TGCCCCTGGG ACAGAGCAAC
    AGCTGTGGCA GCGTGCTCCC CCACTCGCTA GCAACCATGA CCAAAGACTC AGAATCTCGG
    AGGATGCTCA GAGGATATAA TCACCAACAG ATGAGTGAAG GACAAAGGCA GAAGTCTTCT
    GAATTTTACA ACCGCACAGC ATCTGAATCG AATGTCTACT TGAACAGTTT CCACTACCCA
    GATCACAGCT ACAAGGACCA GGCCTTTGAT ACTCTGAGCC TAGACAGCTC TGACAGCATG
    GAGACCAGCA TCTCTGCCTG CTCACCTGAC AATATCTCTA GTGCCAGCAC TTCAAATATT
    GCCCGAATAG AGGAAATGGA GAGACTGTTG AAGCAGGCTC ATGCAGAGAA GACAAGGCTG
    CTTGAATCCA GGGAACGAGA AATGGAAGCC AAGAAACGCG CTCTGGAAGA AGAAAAACGA
    CGTCGAGAAC TCCTGGAAAA ACGGTTGCAA GAAGAAACTA GCCAGAGGCA GAAGTTAATT
    GAAAAGGAAG TAAAAATAAG GGAGAAACAA AGGGCACAGG CTCGACCTCT GACCCGCTAT
    CTGCCTGTGC GGAAGGAAGA CTTTGATTTG CGGAGCCACG TAGAGACTGC TGGCCACAAT
    ATCGATACGT GTTATCATGT GTCAATCACA GAGAAGACCT GCAGAGGATT CCTCATCAAA
    ATGGGTGGAA AAATTAAAAC CTGGAAAAAA CGTTGGTTTG TTTTTGATCG GAACAAGCGC
    ACCTTCTCTT ATTATGCAGA CAAGCATGAA GCTAAATTAA AAGGAGTAAT ATACTTTCAA
    GCCATTGAAG AAGTATATTA TGATCACCTC AAGAACGCTA ATAAGAGTCC TAATCCTTTA
    CTTACCTTTA GCGTCAAGAC ACATGACAGA ATCTACTACA TGGTGGCCCC GTCGCCAGAA
    GCCATGCGGA TCTGGATGGA CGTTATCGTG ACTGGGGCAG AAGGCTACAC TCACTTCTTG
    TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326532.1
    CDS283..4128
    Translation

    Target ORF information:

    RefSeq Version XM_015471046.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471046.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT
    GTAAATGAAA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTC
    CCTGCTGATG CTGATGCTAC TGCCGCTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA
    CAAAGTAAAG AGCATTTTAG AAGTCTGGAA GAAAGGAAAA AACAGCACCA GGAAGGCCTC
    TATCTGAGTG ACACTTTGCC TCGAAAGAAA ACCACACCTT CCATATCCCC ACATTTCAGC
    AGTGCTACTA TGGGGAGAAG CACCACCCCA AAGGGCCACC TGCCCCTGGG ACAGAGCAAC
    AGCTGTGGCA GCGTGCTCCC CCACTCGCTA GCAACCATGA CCAAAGACTC AGAATCTCGG
    AGGATGCTCA GAGGATATAA TCACCAACAG ATGAGTGAAG GACAAAGGCA GAAGTCTTCT
    GAATTTTACA ACCGCACAGC ATCTGAATCG AATGTCTACT TGAACAGTTT CCACTACCCA
    GATCACAGCT ACAAGGACCA GGCCTTTGAT ACTCTGAGCC TAGACAGCTC TGACAGCATG
    GAGACCAGCA TCTCTGCCTG CTCACCTGAC AATATCTCTA GTGCCAGCAC TTCAAATATT
    GCCCGAATAG AGGAAATGGA GAGACTGTTG AAGCAGGCTC ATGCAGAGAA GACAAGGCTG
    CTTGAATCCA GGGAACGAGA AATGGAAGCC AAGAAACGCG CTCTGGAAGA AGAAAAACGA
    CGTCGAGAAC TCCTGGAAAA ACGGTTGCAA GAAGAAACTA GCCAGAGGCA GAAGTTAATT
    GAAAAGGAAG TAAAAATAAG GGAGAAACAA AGGGCACAGG CTCGACCTCT GACCCGCTAT
    CTGCCTGTGC GGAAGGAAGA CTTTGATTTG CGGAGCCACG TAGAGACTGC TGGCCACAAT
    ATCGATACGT GTTATCATGT GTCAATCACA GAGAAGACCT GCAGAGGATT CCTCATCAAA
    ATGGGTGGAA AAATTAAAAC CTGGAAAAAA CGTTGGTTTG TTTTTGATCG GAACAAGCGC
    ACCTTCTCTT ATTATGCAGA CAAGCATGAA GCTAAATTAA AAGGAGTAAT ATACTTTCAA
    GCCATTGAAG AAGTATATTA TGATCACCTC AAGAACGCTA ATAAGAGTCC TAATCCTTTA
    CTTACCTTTA GCGTCAAGAC ACATGACAGA ATCTACTACA TGGTGGCCCC GTCGCCAGAA
    GCCATGCGGA TCTGGATGGA CGTTATCGTG ACTGGGGCAG AAGGCTACAC TCACTTCTTG
    TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15601
    Clone ID Related Accession (Same CDS sequence) XM_015471052.1 , XM_015471052.2
    Accession Version XM_015471052.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103810.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGATGCCGA CCTACTGGAT
    GTTGAGAGCA AACACTTTGA AGACCTGGAA TTCCAGCAGC TTGAGCGTGA AAGCCGTCTA
    GATGAGGAAA AGGAGAACCT GACCCAGCAA CTCCTGCGTG AAGTGGCTGA GTATCAACGG
    AACATTGTTA CTAGAAAGGA AAAAATTTCT GCATTGAAAA AGCAAGCTAA TCACATTGTC
    CAGCAGGCTC AAAGAGAACA AGATCATTTT GTGAAAGAAA AGAATAATTT AATAATGATG
    CTGCAGAGAG AAAAGGAAAA TCTTTGTAAT TTGGAAAAGA AATACTCCAG CCTCTCTGGG
    GGGAAAGGGT TTCCTGTCAC CCCCAATACT CTGAAAGAGG GCTATATCAT TGTAAATGAA
    ATTAATGAGT CATGTGGCAG TTCCACGAAT CTCCCCCCTT CCACTCAGTT CCCTGCTGAT
    GCTGATGCTA CTGCCGCTGG GCCTACCACA GCTGTGCTGA TGAGCCAGCC ACAAAGTAAA
    GAGCATTTTA GAAGTCTGGA AGAAAGGAAA AAACAGCACC AGGAAGGCCT CTATCTGAGT
    GACACTTTGC CTCGAAAGAA AACCACACCT TCCATATCCC CACATTTCAG CAGTGCTACT
    ATGGGGAGAA GCACCACCCC AAAGGGCCAC CTGCCCCTGG GACAGAGCAA CAGCTGTGGC
    AGCGTGCTCC CCCACTCGCT AGCAACCATG ACCAAAGACT CAGAATCTCG GAGGATGCTC
    AGAGGATATA ATCACCAACA GATGAGTGAA GGACAAAGGC AGAAGTCTTC TGAATTTTAC
    AACCGCACAG CATCTGAATC GAATGTCTAC TTGAACAGTT TCCACTACCC AGATCACAGC
    TACAAGGACC AGGCCTTTGA TACTCTGAGC CTAGACAGCT CTGACAGCAT GGAGACCAGC
    ATCTCTGCCT GCTCACCTGA CAATATCTCT AGTGCCAGCA CTTCAAATAT TGCCCGAATA
    GAGGAAATGG AGAGACTGTT GAAGCAGGCT CATGCAGAGA AGACAAGGCT GCTTGAATCC
    AGGGAACGAG AAATGGAAGC CAAGAAACGC GCTCTGGAAG AAGAAAAACG ACGTCGAGAA
    CTCCTGGAAA AACGGTTGCA AGAAGAAACT AGCCAGAGGC AGAAGTTAAT TGAAAAGGAA
    GTAAAAATAA GGGAGAAACA AAGGGCACAG GCTCGACCTC TGACCCGCTA TCTGCCTGTG
    CGGAAGGAAG ACTTTGATTT GCGGAGCCAC GTAGAGACTG CTGGCCACAA TATCGATACG
    TGTTATCATG TGTCAATCAC AGAGAAGACC TGCAGAGGAT TCCTCATCAA AATGGGTGGA
    AAAATTAAAA CCTGGAAAAA ACGTTGGTTT GTTTTTGATC GGAACAAGCG CACCTTCTCT
    TATTATGCAG ACAAGCATGA AGCTAAATTA AAAGGAGTAA TATACTTTCA AGCCATTGAA
    GAAGTATATT ATGATCACCT CAAGAACGCT AATAAGAGTC CTAATCCTTT ACTTACCTTT
    AGCGTCAAGA CACATGACAG AATCTACTAC ATGGTGGCCC CGTCGCCAGA AGCCATGCGG
    ATCTGGATGG ACGTTATCGT GACTGGGGCA GAAGGCTACA CTCACTTCTT GTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326538.1
    CDS325..4041
    Translation

    Target ORF information:

    RefSeq Version XM_015471052.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471052.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGATGCCGA CCTACTGGAT
    GTTGAGAGCA AACACTTTGA AGACCTGGAA TTCCAGCAGC TTGAGCGTGA AAGCCGTCTA
    GATGAGGAAA AGGAGAACCT GACCCAGCAA CTCCTGCGTG AAGTGGCTGA GTATCAACGG
    AACATTGTTA CTAGAAAGGA AAAAATTTCT GCATTGAAAA AGCAAGCTAA TCACATTGTC
    CAGCAGGCTC AAAGAGAACA AGATCATTTT GTGAAAGAAA AGAATAATTT AATAATGATG
    CTGCAGAGAG AAAAGGAAAA TCTTTGTAAT TTGGAAAAGA AATACTCCAG CCTCTCTGGG
    GGGAAAGGGT TTCCTGTCAC CCCCAATACT CTGAAAGAGG GCTATATCAT TGTAAATGAA
    ATTAATGAGT CATGTGGCAG TTCCACGAAT CTCCCCCCTT CCACTCAGTT CCCTGCTGAT
    GCTGATGCTA CTGCCGCTGG GCCTACCACA GCTGTGCTGA TGAGCCAGCC ACAAAGTAAA
    GAGCATTTTA GAAGTCTGGA AGAAAGGAAA AAACAGCACC AGGAAGGCCT CTATCTGAGT
    GACACTTTGC CTCGAAAGAA AACCACACCT TCCATATCCC CACATTTCAG CAGTGCTACT
    ATGGGGAGAA GCACCACCCC AAAGGGCCAC CTGCCCCTGG GACAGAGCAA CAGCTGTGGC
    AGCGTGCTCC CCCACTCGCT AGCAACCATG ACCAAAGACT CAGAATCTCG GAGGATGCTC
    AGAGGATATA ATCACCAACA GATGAGTGAA GGACAAAGGC AGAAGTCTTC TGAATTTTAC
    AACCGCACAG CATCTGAATC GAATGTCTAC TTGAACAGTT TCCACTACCC AGATCACAGC
    TACAAGGACC AGGCCTTTGA TACTCTGAGC CTAGACAGCT CTGACAGCAT GGAGACCAGC
    ATCTCTGCCT GCTCACCTGA CAATATCTCT AGTGCCAGCA CTTCAAATAT TGCCCGAATA
    GAGGAAATGG AGAGACTGTT GAAGCAGGCT CATGCAGAGA AGACAAGGCT GCTTGAATCC
    AGGGAACGAG AAATGGAAGC CAAGAAACGC GCTCTGGAAG AAGAAAAACG ACGTCGAGAA
    CTCCTGGAAA AACGGTTGCA AGAAGAAACT AGCCAGAGGC AGAAGTTAAT TGAAAAGGAA
    GTAAAAATAA GGGAGAAACA AAGGGCACAG GCTCGACCTC TGACCCGCTA TCTGCCTGTG
    CGGAAGGAAG ACTTTGATTT GCGGAGCCAC GTAGAGACTG CTGGCCACAA TATCGATACG
    TGTTATCATG TGTCAATCAC AGAGAAGACC TGCAGAGGAT TCCTCATCAA AATGGGTGGA
    AAAATTAAAA CCTGGAAAAA ACGTTGGTTT GTTTTTGATC GGAACAAGCG CACCTTCTCT
    TATTATGCAG ACAAGCATGA AGCTAAATTA AAAGGAGTAA TATACTTTCA AGCCATTGAA
    GAAGTATATT ATGATCACCT CAAGAACGCT AATAAGAGTC CTAATCCTTT ACTTACCTTT
    AGCGTCAAGA CACATGACAG AATCTACTAC ATGGTGGCCC CGTCGCCAGA AGCCATGCGG
    ATCTGGATGG ACGTTATCGT GACTGGGGCA GAAGGCTACA CTCACTTCTT GTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15601
    Clone ID Related Accession (Same CDS sequence) XM_015471052.1 , XM_015471052.2
    Accession Version XM_015471052.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471052.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGATGCCGA CCTACTGGAT
    GTTGAGAGCA AACACTTTGA AGACCTGGAA TTCCAGCAGC TTGAGCGTGA AAGCCGTCTA
    GATGAGGAAA AGGAGAACCT GACCCAGCAA CTCCTGCGTG AAGTGGCTGA GTATCAACGG
    AACATTGTTA CTAGAAAGGA AAAAATTTCT GCATTGAAAA AGCAAGCTAA TCACATTGTC
    CAGCAGGCTC AAAGAGAACA AGATCATTTT GTGAAAGAAA AGAATAATTT AATAATGATG
    CTGCAGAGAG AAAAGGAAAA TCTTTGTAAT TTGGAAAAGA AATACTCCAG CCTCTCTGGG
    GGGAAAGGGT TTCCTGTCAC CCCCAATACT CTGAAAGAGG GCTATATCAT TGTAAATGAA
    ATTAATGAGT CATGTGGCAG TTCCACGAAT CTCCCCCCTT CCACTCAGTT CCCTGCTGAT
    GCTGATGCTA CTGCCGCTGG GCCTACCACA GCTGTGCTGA TGAGCCAGCC ACAAAGTAAA
    GAGCATTTTA GAAGTCTGGA AGAAAGGAAA AAACAGCACC AGGAAGGCCT CTATCTGAGT
    GACACTTTGC CTCGAAAGAA AACCACACCT TCCATATCCC CACATTTCAG CAGTGCTACT
    ATGGGGAGAA GCACCACCCC AAAGGGCCAC CTGCCCCTGG GACAGAGCAA CAGCTGTGGC
    AGCGTGCTCC CCCACTCGCT AGCAACCATG ACCAAAGACT CAGAATCTCG GAGGATGCTC
    AGAGGATATA ATCACCAACA GATGAGTGAA GGACAAAGGC AGAAGTCTTC TGAATTTTAC
    AACCGCACAG CATCTGAATC GAATGTCTAC TTGAACAGTT TCCACTACCC AGATCACAGC
    TACAAGGACC AGGCCTTTGA TACTCTGAGC CTAGACAGCT CTGACAGCAT GGAGACCAGC
    ATCTCTGCCT GCTCACCTGA CAATATCTCT AGTGCCAGCA CTTCAAATAT TGCCCGAATA
    GAGGAAATGG AGAGACTGTT GAAGCAGGCT CATGCAGAGA AGACAAGGCT GCTTGAATCC
    AGGGAACGAG AAATGGAAGC CAAGAAACGC GCTCTGGAAG AAGAAAAACG ACGTCGAGAA
    CTCCTGGAAA AACGGTTGCA AGAAGAAACT AGCCAGAGGC AGAAGTTAAT TGAAAAGGAA
    GTAAAAATAA GGGAGAAACA AAGGGCACAG GCTCGACCTC TGACCCGCTA TCTGCCTGTG
    CGGAAGGAAG ACTTTGATTT GCGGAGCCAC GTAGAGACTG CTGGCCACAA TATCGATACG
    TGTTATCATG TGTCAATCAC AGAGAAGACC TGCAGAGGAT TCCTCATCAA AATGGGTGGA
    AAAATTAAAA CCTGGAAAAA ACGTTGGTTT GTTTTTGATC GGAACAAGCG CACCTTCTCT
    TATTATGCAG ACAAGCATGA AGCTAAATTA AAAGGAGTAA TATACTTTCA AGCCATTGAA
    GAAGTATATT ATGATCACCT CAAGAACGCT AATAAGAGTC CTAATCCTTT ACTTACCTTT
    AGCGTCAAGA CACATGACAG AATCTACTAC ATGGTGGCCC CGTCGCCAGA AGCCATGCGG
    ATCTGGATGG ACGTTATCGT GACTGGGGCA GAAGGCTACA CTCACTTCTT GTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326538.1
    CDS283..3999
    Translation

    Target ORF information:

    RefSeq Version XM_015471052.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471052.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGATGCCGA CCTACTGGAT
    GTTGAGAGCA AACACTTTGA AGACCTGGAA TTCCAGCAGC TTGAGCGTGA AAGCCGTCTA
    GATGAGGAAA AGGAGAACCT GACCCAGCAA CTCCTGCGTG AAGTGGCTGA GTATCAACGG
    AACATTGTTA CTAGAAAGGA AAAAATTTCT GCATTGAAAA AGCAAGCTAA TCACATTGTC
    CAGCAGGCTC AAAGAGAACA AGATCATTTT GTGAAAGAAA AGAATAATTT AATAATGATG
    CTGCAGAGAG AAAAGGAAAA TCTTTGTAAT TTGGAAAAGA AATACTCCAG CCTCTCTGGG
    GGGAAAGGGT TTCCTGTCAC CCCCAATACT CTGAAAGAGG GCTATATCAT TGTAAATGAA
    ATTAATGAGT CATGTGGCAG TTCCACGAAT CTCCCCCCTT CCACTCAGTT CCCTGCTGAT
    GCTGATGCTA CTGCCGCTGG GCCTACCACA GCTGTGCTGA TGAGCCAGCC ACAAAGTAAA
    GAGCATTTTA GAAGTCTGGA AGAAAGGAAA AAACAGCACC AGGAAGGCCT CTATCTGAGT
    GACACTTTGC CTCGAAAGAA AACCACACCT TCCATATCCC CACATTTCAG CAGTGCTACT
    ATGGGGAGAA GCACCACCCC AAAGGGCCAC CTGCCCCTGG GACAGAGCAA CAGCTGTGGC
    AGCGTGCTCC CCCACTCGCT AGCAACCATG ACCAAAGACT CAGAATCTCG GAGGATGCTC
    AGAGGATATA ATCACCAACA GATGAGTGAA GGACAAAGGC AGAAGTCTTC TGAATTTTAC
    AACCGCACAG CATCTGAATC GAATGTCTAC TTGAACAGTT TCCACTACCC AGATCACAGC
    TACAAGGACC AGGCCTTTGA TACTCTGAGC CTAGACAGCT CTGACAGCAT GGAGACCAGC
    ATCTCTGCCT GCTCACCTGA CAATATCTCT AGTGCCAGCA CTTCAAATAT TGCCCGAATA
    GAGGAAATGG AGAGACTGTT GAAGCAGGCT CATGCAGAGA AGACAAGGCT GCTTGAATCC
    AGGGAACGAG AAATGGAAGC CAAGAAACGC GCTCTGGAAG AAGAAAAACG ACGTCGAGAA
    CTCCTGGAAA AACGGTTGCA AGAAGAAACT AGCCAGAGGC AGAAGTTAAT TGAAAAGGAA
    GTAAAAATAA GGGAGAAACA AAGGGCACAG GCTCGACCTC TGACCCGCTA TCTGCCTGTG
    CGGAAGGAAG ACTTTGATTT GCGGAGCCAC GTAGAGACTG CTGGCCACAA TATCGATACG
    TGTTATCATG TGTCAATCAC AGAGAAGACC TGCAGAGGAT TCCTCATCAA AATGGGTGGA
    AAAATTAAAA CCTGGAAAAA ACGTTGGTTT GTTTTTGATC GGAACAAGCG CACCTTCTCT
    TATTATGCAG ACAAGCATGA AGCTAAATTA AAAGGAGTAA TATACTTTCA AGCCATTGAA
    GAAGTATATT ATGATCACCT CAAGAACGCT AATAAGAGTC CTAATCCTTT ACTTACCTTT
    AGCGTCAAGA CACATGACAG AATCTACTAC ATGGTGGCCC CGTCGCCAGA AGCCATGCGG
    ATCTGGATGG ACGTTATCGT GACTGGGGCA GAAGGCTACA CTCACTTCTT GTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218690
    Clone ID Related Accession (Same CDS sequence) XM_024977109.1
    Accession Version XM_024977109.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGG CTATATCATT GTAAATGAAA TTAATGAGTC ATGTGGCAGT
    TCCACGAATC TCCCCCCTTC CACTCAGTTC CCTGCTGATG CTGATGCTAC TGCCGCTGGG
    CCTACCACAG CTGTGCTGAT GAGCCAGCCA CAAAGTAAAG AGCAAAGATC GTCTGTCTGT
    CCTGGATTTA TGTTTCCTTC CACTCTTTCT TCTCATCCTA TCACTCAACC TCCATCAGCC
    CCTTGGCCTG AAGCCGTGGC TACCTTTGTC GATTCTTTTC CTTTAAATGA CACACCTCCT
    CCTCTACCAG CTAAGAAACA CAGGAGGCAA CAGCAGCAGG ACCAGCAGCA TTTTAGAAGT
    CTGGAAGAAA GGAAAAAACA GCACCAGGAA GGCCTCTATC TGAGTGACAC TTTGCCTCGA
    AAGAAAACCA CACCTTCCAT ATCCCCACAT TTCAGCAGTG CTACTATGGG GAGAAGCACC
    ACCCCAAAGG GCCACCTGCC CCTGGGACAG AGCAACAGCT GTGGCAGCGT GCTCCCCCAC
    TCGCTAGCAA CCATGACCAA AGACTCAGAA TCTCGGAGGA TGCTCAGAGG AAAGTCCAAG
    AAAGGAAGAA TGAATTTCTC AGCAGGAGCA GTTAGTAATT CCAAAAGGTC AAGTGTCAAA
    GGATATAATC ACCAACAGAT GAGTGAAGGA CAAAGGCAGA AGTCTTCTGA ATTTTACAAC
    CGCACAGCAT CTGAATCGAA TGTCTACTTG AACAGTTTCC ACTACCCAGA TCACAGCTAC
    AAGGACCAGG CCTTTGATAC TCTGAGCCTA GACAGCTCTG ACAGCATGGA GACCAGCATC
    TCTGCCTGCT CACCTGACAA TATCTCTAGT GCCAGCACTT CAAATATTGC CCGAATAGAG
    GAAATGGAGA GACTGTTGAA GCAGGCTCAT GCAGAGAAGA CAAGGCTGCT TGAATCCAGG
    GAACGAGAAA TGGAAGCCAA GAAACGCGCT CTGGAAGAAG AAAAACGACG TCGAGAACTC
    CTGGAAAAAC GGTTGCAAGA AGAAACTAGC CAGAGGCAGA AGTTAATTGA AAAGGAAGTA
    AAAATAAGGG AGAAACAAAG GGCACAGGCT CGACCTCTGA CCCGCTATCT GCCTGTGCGG
    AAGGAAGACT TTGATTTGCG GAGCCACGTA GAGACTGCTG GCCACAATAT CGATACGTGT
    TATCATGTGT CAATCACAGA GAAGACCTGC AGAGGATTCC TCATCAAAAT GGGTGGAAAA
    ATTAAAACCT GGAAAAAACG TTGGTTTGTT TTTGATCGGA ACAAGCGCAC CTTCTCTTAT
    TATGCAGACA AGCATGAAGC TAAATTAAAA GGAGTAATAT ACTTTCAAGC CATTGAAGAA
    GTATATTATG ATCACCTCAA GAACGCTAAT AAGAGTCCTA ATCCTTTACT TACCTTTAGC
    GTCAAGACAC ATGACAGAAT CTACTACATG GTGGCCCCGT CGCCAGAAGC CATGCGGATC
    TGGATGGACG TTATCGTGAC TGGGGCAGAA GGCTACACTC ACTTCTTGTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832877.1
    CDS283..4296
    Translation

    Target ORF information:

    RefSeq Version XM_024977109.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977109.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGG CTATATCATT GTAAATGAAA TTAATGAGTC ATGTGGCAGT
    TCCACGAATC TCCCCCCTTC CACTCAGTTC CCTGCTGATG CTGATGCTAC TGCCGCTGGG
    CCTACCACAG CTGTGCTGAT GAGCCAGCCA CAAAGTAAAG AGCAAAGATC GTCTGTCTGT
    CCTGGATTTA TGTTTCCTTC CACTCTTTCT TCTCATCCTA TCACTCAACC TCCATCAGCC
    CCTTGGCCTG AAGCCGTGGC TACCTTTGTC GATTCTTTTC CTTTAAATGA CACACCTCCT
    CCTCTACCAG CTAAGAAACA CAGGAGGCAA CAGCAGCAGG ACCAGCAGCA TTTTAGAAGT
    CTGGAAGAAA GGAAAAAACA GCACCAGGAA GGCCTCTATC TGAGTGACAC TTTGCCTCGA
    AAGAAAACCA CACCTTCCAT ATCCCCACAT TTCAGCAGTG CTACTATGGG GAGAAGCACC
    ACCCCAAAGG GCCACCTGCC CCTGGGACAG AGCAACAGCT GTGGCAGCGT GCTCCCCCAC
    TCGCTAGCAA CCATGACCAA AGACTCAGAA TCTCGGAGGA TGCTCAGAGG AAAGTCCAAG
    AAAGGAAGAA TGAATTTCTC AGCAGGAGCA GTTAGTAATT CCAAAAGGTC AAGTGTCAAA
    GGATATAATC ACCAACAGAT GAGTGAAGGA CAAAGGCAGA AGTCTTCTGA ATTTTACAAC
    CGCACAGCAT CTGAATCGAA TGTCTACTTG AACAGTTTCC ACTACCCAGA TCACAGCTAC
    AAGGACCAGG CCTTTGATAC TCTGAGCCTA GACAGCTCTG ACAGCATGGA GACCAGCATC
    TCTGCCTGCT CACCTGACAA TATCTCTAGT GCCAGCACTT CAAATATTGC CCGAATAGAG
    GAAATGGAGA GACTGTTGAA GCAGGCTCAT GCAGAGAAGA CAAGGCTGCT TGAATCCAGG
    GAACGAGAAA TGGAAGCCAA GAAACGCGCT CTGGAAGAAG AAAAACGACG TCGAGAACTC
    CTGGAAAAAC GGTTGCAAGA AGAAACTAGC CAGAGGCAGA AGTTAATTGA AAAGGAAGTA
    AAAATAAGGG AGAAACAAAG GGCACAGGCT CGACCTCTGA CCCGCTATCT GCCTGTGCGG
    AAGGAAGACT TTGATTTGCG GAGCCACGTA GAGACTGCTG GCCACAATAT CGATACGTGT
    TATCATGTGT CAATCACAGA GAAGACCTGC AGAGGATTCC TCATCAAAAT GGGTGGAAAA
    ATTAAAACCT GGAAAAAACG TTGGTTTGTT TTTGATCGGA ACAAGCGCAC CTTCTCTTAT
    TATGCAGACA AGCATGAAGC TAAATTAAAA GGAGTAATAT ACTTTCAAGC CATTGAAGAA
    GTATATTATG ATCACCTCAA GAACGCTAAT AAGAGTCCTA ATCCTTTACT TACCTTTAGC
    GTCAAGACAC ATGACAGAAT CTACTACATG GTGGCCCCGT CGCCAGAAGC CATGCGGATC
    TGGATGGACG TTATCGTGAC TGGGGCAGAA GGCTACACTC ACTTCTTGTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218691
    Clone ID Related Accession (Same CDS sequence) XM_024977397.1
    Accession Version XM_024977397.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 2 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGG CCACCTGCCC CTGGGACAGA GCAACAGCTG TGGCAGCGTG
    CTCCCCCACT CGCTAGCAAC CATGACCAAA GACTCAGAAT CTCGGAGGAT GCTCAGAGGA
    TATAATCACC AACAGATGAG TGAAGGACAA AGGCAGAAGT CTTCTGAATT TTACAACCGC
    ACAGCATCTG AATCGAATGT CTACTTGAAC AGTTTCCACT ACCCAGATCA CAGCTACAAG
    GACCAGGCCT TTGATACTCT GAGCCTAGAC AGCTCTGACA GCATGGAGAC CAGCATCTCT
    GCCTGCTCAC CTGACAATAT CTCTAGTGCC AGCACTTCAA ATATTGCCCG AATAGAGGAA
    ATGGAGAGAC TGTTGAAGCA GGCTCATGCA GAGAAGACAA GGCTGCTTGA ATCCAGGGAA
    CGAGAAATGG AAGCCAAGAA ACGCGCTCTG GAAGAAGAAA AACGACGTCG AGAACTCCTG
    GAAAAACGGT TGCAAGAAGA AACTAGCCAG AGGCAGAAGT TAATTGAAAA GGAAGTAAAA
    ATAAGGGAGA AACAAAGGGC ACAGGCTCGA CCTCTGACCC GCTATCTGCC TGTGCGGAAG
    GAAGACTTTG ATTTGCGGAG CCACGTAGAG ACTGCTGGCC ACAATATCGA TACGTGTTAT
    CATGTGTCAA TCACAGAGAA GACCTGCAGA GGATTCCTCA TCAAAATGGG TGGAAAAATT
    AAAACCTGGA AAAAACGTTG GTTTGTTTTT GATCGGAACA AGCGCACCTT CTCTTATTAT
    GCAGACAAGC ATGAAGCTAA ATTAAAAGGA GTAATATACT TTCAAGCCAT TGAAGAAGTA
    TATTATGATC ACCTCAAGAA CGCTAATAAG AGTCCTAATC CTTTACTTAC CTTTAGCGTC
    AAGACACATG ACAGAATCTA CTACATGGTG GCCCCGTCGC CAGAAGCCAT GCGGATCTGG
    ATGGACGTTA TCGTGACTGG GGCAGAAGGC TACACTCACT TCTTGTTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833165.1
    CDS283..3753
    Translation

    Target ORF information:

    RefSeq Version XM_024977397.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977397.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGG CCACCTGCCC CTGGGACAGA GCAACAGCTG TGGCAGCGTG
    CTCCCCCACT CGCTAGCAAC CATGACCAAA GACTCAGAAT CTCGGAGGAT GCTCAGAGGA
    TATAATCACC AACAGATGAG TGAAGGACAA AGGCAGAAGT CTTCTGAATT TTACAACCGC
    ACAGCATCTG AATCGAATGT CTACTTGAAC AGTTTCCACT ACCCAGATCA CAGCTACAAG
    GACCAGGCCT TTGATACTCT GAGCCTAGAC AGCTCTGACA GCATGGAGAC CAGCATCTCT
    GCCTGCTCAC CTGACAATAT CTCTAGTGCC AGCACTTCAA ATATTGCCCG AATAGAGGAA
    ATGGAGAGAC TGTTGAAGCA GGCTCATGCA GAGAAGACAA GGCTGCTTGA ATCCAGGGAA
    CGAGAAATGG AAGCCAAGAA ACGCGCTCTG GAAGAAGAAA AACGACGTCG AGAACTCCTG
    GAAAAACGGT TGCAAGAAGA AACTAGCCAG AGGCAGAAGT TAATTGAAAA GGAAGTAAAA
    ATAAGGGAGA AACAAAGGGC ACAGGCTCGA CCTCTGACCC GCTATCTGCC TGTGCGGAAG
    GAAGACTTTG ATTTGCGGAG CCACGTAGAG ACTGCTGGCC ACAATATCGA TACGTGTTAT
    CATGTGTCAA TCACAGAGAA GACCTGCAGA GGATTCCTCA TCAAAATGGG TGGAAAAATT
    AAAACCTGGA AAAAACGTTG GTTTGTTTTT GATCGGAACA AGCGCACCTT CTCTTATTAT
    GCAGACAAGC ATGAAGCTAA ATTAAAAGGA GTAATATACT TTCAAGCCAT TGAAGAAGTA
    TATTATGATC ACCTCAAGAA CGCTAATAAG AGTCCTAATC CTTTACTTAC CTTTAGCGTC
    AAGACACATG ACAGAATCTA CTACATGGTG GCCCCGTCGC CAGAAGCCAT GCGGATCTGG
    ATGGACGTTA TCGTGACTGG GGCAGAAGGC TACACTCACT TCTTGTTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47504
    Clone ID Related Accession (Same CDS sequence) XM_024977359.1
    Accession Version XM_024977359.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CCACCTGCCC
    CTGGGACAGA GCAACAGCTG TGGCAGCGTG CTCCCCCACT CGCTAGCAAC CATGACCAAA
    GACTCAGAAT CTCGGAGGAT GCTCAGAGGA TATAATCACC AACAGATGAG TGAAGGACAA
    AGGCAGAAGT CTTCTGAATT TTACAACCGC ACAGCATCTG AATCGAATGT CTACTTGAAC
    AGTTTCCACT ACCCAGATCA CAGCTACAAG GACCAGGCCT TTGATACTCT GAGCCTAGAC
    AGCTCTGACA GCATGGAGAC CAGCATCTCT GCCTGCTCAC CTGACAATAT CTCTAGTGCC
    AGCACTTCAA ATATTGCCCG AATAGAGGAA ATGGAGAGAC TGTTGAAGCA GGCTCATGCA
    GAGAAGACAA GGCTGCTTGA ATCCAGGGAA CGAGAAATGG AAGCCAAGAA ACGCGCTCTG
    GAAGAAGAAA AACGACGTCG AGAACTCCTG GAAAAACGGT TGCAAGAAGA AACTAGCCAG
    AGGCAGAAGT TAATTGAAAA GGAAGTAAAA ATAAGGGAGA AACAAAGGGC ACAGGCTCGA
    CCTCTGACCC GCTATCTGCC TGTGCGGAAG GAAGACTTTG ATTTGCGGAG CCACGTAGAG
    ACTGCTGGCC ACAATATCGA TACGTGTTAT CATGTGTCAA TCACAGAGAA GACCTGCAGA
    GGATTCCTCA TCAAAATGGG TGGAAAAATT AAAACCTGGA AAAAACGTTG GTTTGTTTTT
    GATCGGAACA AGCGCACCTT CTCTTATTAT GCAGACAAGC ATGAAGCTAA ATTAAAAGGA
    GTAATATACT TTCAAGCCAT TGAAGAAGTA TATTATGATC ACCTCAAGAA CGCTAATAAG
    AGTCCTAATC CTTTACTTAC CTTTAGCGTC AAGACACATG ACAGAATCTA CTACATGGTG
    GCCCCGTCGC CAGAAGCCAT GCGGATCTGG ATGGACGTTA TCGTGACTGG GGCAGAAGGC
    TACACTCACT TCTTGTTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833127.1
    CDS283..3843
    Translation

    Target ORF information:

    RefSeq Version XM_024977359.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977359.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CCACCTGCCC
    CTGGGACAGA GCAACAGCTG TGGCAGCGTG CTCCCCCACT CGCTAGCAAC CATGACCAAA
    GACTCAGAAT CTCGGAGGAT GCTCAGAGGA TATAATCACC AACAGATGAG TGAAGGACAA
    AGGCAGAAGT CTTCTGAATT TTACAACCGC ACAGCATCTG AATCGAATGT CTACTTGAAC
    AGTTTCCACT ACCCAGATCA CAGCTACAAG GACCAGGCCT TTGATACTCT GAGCCTAGAC
    AGCTCTGACA GCATGGAGAC CAGCATCTCT GCCTGCTCAC CTGACAATAT CTCTAGTGCC
    AGCACTTCAA ATATTGCCCG AATAGAGGAA ATGGAGAGAC TGTTGAAGCA GGCTCATGCA
    GAGAAGACAA GGCTGCTTGA ATCCAGGGAA CGAGAAATGG AAGCCAAGAA ACGCGCTCTG
    GAAGAAGAAA AACGACGTCG AGAACTCCTG GAAAAACGGT TGCAAGAAGA AACTAGCCAG
    AGGCAGAAGT TAATTGAAAA GGAAGTAAAA ATAAGGGAGA AACAAAGGGC ACAGGCTCGA
    CCTCTGACCC GCTATCTGCC TGTGCGGAAG GAAGACTTTG ATTTGCGGAG CCACGTAGAG
    ACTGCTGGCC ACAATATCGA TACGTGTTAT CATGTGTCAA TCACAGAGAA GACCTGCAGA
    GGATTCCTCA TCAAAATGGG TGGAAAAATT AAAACCTGGA AAAAACGTTG GTTTGTTTTT
    GATCGGAACA AGCGCACCTT CTCTTATTAT GCAGACAAGC ATGAAGCTAA ATTAAAAGGA
    GTAATATACT TTCAAGCCAT TGAAGAAGTA TATTATGATC ACCTCAAGAA CGCTAATAAG
    AGTCCTAATC CTTTACTTAC CTTTAGCGTC AAGACACATG ACAGAATCTA CTACATGGTG
    GCCCCGTCGC CAGAAGCCAT GCGGATCTGG ATGGACGTTA TCGTGACTGG GGCAGAAGGC
    TACACTCACT TCTTGTTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87685
    Clone ID Related Accession (Same CDS sequence) XM_015471035.1 , XM_015471036.1 , XM_015471035.2
    Accession Version XM_015471035.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4104bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103810.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT
    GTAAATGAAA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTC
    CCTGCTGATG CTGATGCTAC TGCCGCTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA
    CAAAGTAAAG AGCAAAGATC GTCTGTCTGT CCTGGATTTA TGTTTCCTTC CACTCTTTCT
    TCTCATCCTA TCACTCAACC TCCATCAGCC CCTTGGCCTG AAGCCGTGGC TACCTTTGTC
    GATTCTTTTC CTTTAAATGA CACACCTCCT CCTCTACCAG CTAAGAAACA CAGGAGGCAA
    CAGCAGCAGG ACCAGCAGCA TTTTAGAAGT CTGGAAGAAA GGAAAAAACA GCACCAGGAA
    GGCCTCTATC TGAGTGACAC TTTGCCTCGA AAGAAAACCA CACCTTCCAT ATCCCCACAT
    TTCAGCAGTG CTACTATGGG GAGAAGCACC ACCCCAAAGG GCCACCTGCC CCTGGGACAG
    AGCAACAGCT GTGGCAGCGT GCTCCCCCAC TCGCTAGCAA CCATGACCAA AGACTCAGAA
    TCTCGGAGGA TGCTCAGAGG AAAGTCCAAG AAAGGAAGAA TGAATTTCTC AGCAGGAGCA
    GTTAGTAATT CCAAAAGGTC AAGTGTCAAA GGATATAATC ACCAACAGAT GAGTGAAGGA
    CAAAGGCAGA AGTCTTCTGA ATTTTACAAC CGCACAGCAT CTGAATCGAA TGTCTACTTG
    AACAGTTTCC ACTACCCAGA TCACAGCTAC AAGGACCAGG CCTTTGATAC TCTGAGCCTA
    GACAGCTCTG ACAGCATGGA GACCAGCATC TCTGCCTGCT CACCTGACAA TATCTCTAGT
    GCCAGCACTT CAAATATTGC CCGAATAGAG GAAATGGAGA GACTGTTGAA GCAGGCTCAT
    GCAGAGAAGA CAAGGCTGCT TGAATCCAGG GAACGAGAAA TGGAAGCCAA GAAACGCGCT
    CTGGAAGAAG AAAAACGACG TCGAGAACTC CTGGAAAAAC GGTTGCAAGA AGAAACTAGC
    CAGAGGCAGA AGTTAATTGA AAAGGAAGTA AAAATAAGGG AGAAACAAAG GGCACAGGCT
    CGACCTCTGA CCCGCTATCT GCCTGTGCGG AAGGAAGACT TTGATTTGCG GAGCCACGTA
    GAGACTGCTG GCCACAATAT CGATACGTGT TATCATGTGT CAATCACAGA GAAGACCTGC
    AGAGGATTCC TCATCAAAAT GGGTGGAAAA ATTAAAACCT GGAAAAAACG TTGGTTTGTT
    TTTGATCGGA ACAAGCGCAC CTTCTCTTAT TATGCAGACA AGCATGAAGC TAAATTAAAA
    GGAGTAATAT ACTTTCAAGC CATTGAAGAA GTATATTATG ATCACCTCAA GAACGCTAAT
    AAGAGTCCTA ATCCTTTACT TACCTTTAGC GTCAAGACAC ATGACAGAAT CTACTACATG
    GTGGCCCCGT CGCCAGAAGC CATGCGGATC TGGATGGACG TTATCGTGAC TGGGGCAGAA
    GGCTACACTC ACTTCTTGTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326521.1
    CDS331..4434
    Translation

    Target ORF information:

    RefSeq Version XM_015471035.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471035.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT
    GTAAATGAAA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTC
    CCTGCTGATG CTGATGCTAC TGCCGCTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA
    CAAAGTAAAG AGCAAAGATC GTCTGTCTGT CCTGGATTTA TGTTTCCTTC CACTCTTTCT
    TCTCATCCTA TCACTCAACC TCCATCAGCC CCTTGGCCTG AAGCCGTGGC TACCTTTGTC
    GATTCTTTTC CTTTAAATGA CACACCTCCT CCTCTACCAG CTAAGAAACA CAGGAGGCAA
    CAGCAGCAGG ACCAGCAGCA TTTTAGAAGT CTGGAAGAAA GGAAAAAACA GCACCAGGAA
    GGCCTCTATC TGAGTGACAC TTTGCCTCGA AAGAAAACCA CACCTTCCAT ATCCCCACAT
    TTCAGCAGTG CTACTATGGG GAGAAGCACC ACCCCAAAGG GCCACCTGCC CCTGGGACAG
    AGCAACAGCT GTGGCAGCGT GCTCCCCCAC TCGCTAGCAA CCATGACCAA AGACTCAGAA
    TCTCGGAGGA TGCTCAGAGG AAAGTCCAAG AAAGGAAGAA TGAATTTCTC AGCAGGAGCA
    GTTAGTAATT CCAAAAGGTC AAGTGTCAAA GGATATAATC ACCAACAGAT GAGTGAAGGA
    CAAAGGCAGA AGTCTTCTGA ATTTTACAAC CGCACAGCAT CTGAATCGAA TGTCTACTTG
    AACAGTTTCC ACTACCCAGA TCACAGCTAC AAGGACCAGG CCTTTGATAC TCTGAGCCTA
    GACAGCTCTG ACAGCATGGA GACCAGCATC TCTGCCTGCT CACCTGACAA TATCTCTAGT
    GCCAGCACTT CAAATATTGC CCGAATAGAG GAAATGGAGA GACTGTTGAA GCAGGCTCAT
    GCAGAGAAGA CAAGGCTGCT TGAATCCAGG GAACGAGAAA TGGAAGCCAA GAAACGCGCT
    CTGGAAGAAG AAAAACGACG TCGAGAACTC CTGGAAAAAC GGTTGCAAGA AGAAACTAGC
    CAGAGGCAGA AGTTAATTGA AAAGGAAGTA AAAATAAGGG AGAAACAAAG GGCACAGGCT
    CGACCTCTGA CCCGCTATCT GCCTGTGCGG AAGGAAGACT TTGATTTGCG GAGCCACGTA
    GAGACTGCTG GCCACAATAT CGATACGTGT TATCATGTGT CAATCACAGA GAAGACCTGC
    AGAGGATTCC TCATCAAAAT GGGTGGAAAA ATTAAAACCT GGAAAAAACG TTGGTTTGTT
    TTTGATCGGA ACAAGCGCAC CTTCTCTTAT TATGCAGACA AGCATGAAGC TAAATTAAAA
    GGAGTAATAT ACTTTCAAGC CATTGAAGAA GTATATTATG ATCACCTCAA GAACGCTAAT
    AAGAGTCCTA ATCCTTTACT TACCTTTAGC GTCAAGACAC ATGACAGAAT CTACTACATG
    GTGGCCCCGT CGCCAGAAGC CATGCGGATC TGGATGGACG TTATCGTGAC TGGGGCAGAA
    GGCTACACTC ACTTCTTGTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87685
    Clone ID Related Accession (Same CDS sequence) XM_015471035.1 , XM_015471036.1 , XM_015471035.2
    Accession Version XM_015471036.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4104bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103810.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT
    GTAAATGAAA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTC
    CCTGCTGATG CTGATGCTAC TGCCGCTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA
    CAAAGTAAAG AGCAAAGATC GTCTGTCTGT CCTGGATTTA TGTTTCCTTC CACTCTTTCT
    TCTCATCCTA TCACTCAACC TCCATCAGCC CCTTGGCCTG AAGCCGTGGC TACCTTTGTC
    GATTCTTTTC CTTTAAATGA CACACCTCCT CCTCTACCAG CTAAGAAACA CAGGAGGCAA
    CAGCAGCAGG ACCAGCAGCA TTTTAGAAGT CTGGAAGAAA GGAAAAAACA GCACCAGGAA
    GGCCTCTATC TGAGTGACAC TTTGCCTCGA AAGAAAACCA CACCTTCCAT ATCCCCACAT
    TTCAGCAGTG CTACTATGGG GAGAAGCACC ACCCCAAAGG GCCACCTGCC CCTGGGACAG
    AGCAACAGCT GTGGCAGCGT GCTCCCCCAC TCGCTAGCAA CCATGACCAA AGACTCAGAA
    TCTCGGAGGA TGCTCAGAGG AAAGTCCAAG AAAGGAAGAA TGAATTTCTC AGCAGGAGCA
    GTTAGTAATT CCAAAAGGTC AAGTGTCAAA GGATATAATC ACCAACAGAT GAGTGAAGGA
    CAAAGGCAGA AGTCTTCTGA ATTTTACAAC CGCACAGCAT CTGAATCGAA TGTCTACTTG
    AACAGTTTCC ACTACCCAGA TCACAGCTAC AAGGACCAGG CCTTTGATAC TCTGAGCCTA
    GACAGCTCTG ACAGCATGGA GACCAGCATC TCTGCCTGCT CACCTGACAA TATCTCTAGT
    GCCAGCACTT CAAATATTGC CCGAATAGAG GAAATGGAGA GACTGTTGAA GCAGGCTCAT
    GCAGAGAAGA CAAGGCTGCT TGAATCCAGG GAACGAGAAA TGGAAGCCAA GAAACGCGCT
    CTGGAAGAAG AAAAACGACG TCGAGAACTC CTGGAAAAAC GGTTGCAAGA AGAAACTAGC
    CAGAGGCAGA AGTTAATTGA AAAGGAAGTA AAAATAAGGG AGAAACAAAG GGCACAGGCT
    CGACCTCTGA CCCGCTATCT GCCTGTGCGG AAGGAAGACT TTGATTTGCG GAGCCACGTA
    GAGACTGCTG GCCACAATAT CGATACGTGT TATCATGTGT CAATCACAGA GAAGACCTGC
    AGAGGATTCC TCATCAAAAT GGGTGGAAAA ATTAAAACCT GGAAAAAACG TTGGTTTGTT
    TTTGATCGGA ACAAGCGCAC CTTCTCTTAT TATGCAGACA AGCATGAAGC TAAATTAAAA
    GGAGTAATAT ACTTTCAAGC CATTGAAGAA GTATATTATG ATCACCTCAA GAACGCTAAT
    AAGAGTCCTA ATCCTTTACT TACCTTTAGC GTCAAGACAC ATGACAGAAT CTACTACATG
    GTGGCCCCGT CGCCAGAAGC CATGCGGATC TGGATGGACG TTATCGTGAC TGGGGCAGAA
    GGCTACACTC ACTTCTTGTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326522.1
    CDS530..4633
    Translation

    Target ORF information:

    RefSeq Version XM_015471036.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471036.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT
    GTAAATGAAA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTC
    CCTGCTGATG CTGATGCTAC TGCCGCTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA
    CAAAGTAAAG AGCAAAGATC GTCTGTCTGT CCTGGATTTA TGTTTCCTTC CACTCTTTCT
    TCTCATCCTA TCACTCAACC TCCATCAGCC CCTTGGCCTG AAGCCGTGGC TACCTTTGTC
    GATTCTTTTC CTTTAAATGA CACACCTCCT CCTCTACCAG CTAAGAAACA CAGGAGGCAA
    CAGCAGCAGG ACCAGCAGCA TTTTAGAAGT CTGGAAGAAA GGAAAAAACA GCACCAGGAA
    GGCCTCTATC TGAGTGACAC TTTGCCTCGA AAGAAAACCA CACCTTCCAT ATCCCCACAT
    TTCAGCAGTG CTACTATGGG GAGAAGCACC ACCCCAAAGG GCCACCTGCC CCTGGGACAG
    AGCAACAGCT GTGGCAGCGT GCTCCCCCAC TCGCTAGCAA CCATGACCAA AGACTCAGAA
    TCTCGGAGGA TGCTCAGAGG AAAGTCCAAG AAAGGAAGAA TGAATTTCTC AGCAGGAGCA
    GTTAGTAATT CCAAAAGGTC AAGTGTCAAA GGATATAATC ACCAACAGAT GAGTGAAGGA
    CAAAGGCAGA AGTCTTCTGA ATTTTACAAC CGCACAGCAT CTGAATCGAA TGTCTACTTG
    AACAGTTTCC ACTACCCAGA TCACAGCTAC AAGGACCAGG CCTTTGATAC TCTGAGCCTA
    GACAGCTCTG ACAGCATGGA GACCAGCATC TCTGCCTGCT CACCTGACAA TATCTCTAGT
    GCCAGCACTT CAAATATTGC CCGAATAGAG GAAATGGAGA GACTGTTGAA GCAGGCTCAT
    GCAGAGAAGA CAAGGCTGCT TGAATCCAGG GAACGAGAAA TGGAAGCCAA GAAACGCGCT
    CTGGAAGAAG AAAAACGACG TCGAGAACTC CTGGAAAAAC GGTTGCAAGA AGAAACTAGC
    CAGAGGCAGA AGTTAATTGA AAAGGAAGTA AAAATAAGGG AGAAACAAAG GGCACAGGCT
    CGACCTCTGA CCCGCTATCT GCCTGTGCGG AAGGAAGACT TTGATTTGCG GAGCCACGTA
    GAGACTGCTG GCCACAATAT CGATACGTGT TATCATGTGT CAATCACAGA GAAGACCTGC
    AGAGGATTCC TCATCAAAAT GGGTGGAAAA ATTAAAACCT GGAAAAAACG TTGGTTTGTT
    TTTGATCGGA ACAAGCGCAC CTTCTCTTAT TATGCAGACA AGCATGAAGC TAAATTAAAA
    GGAGTAATAT ACTTTCAAGC CATTGAAGAA GTATATTATG ATCACCTCAA GAACGCTAAT
    AAGAGTCCTA ATCCTTTACT TACCTTTAGC GTCAAGACAC ATGACAGAAT CTACTACATG
    GTGGCCCCGT CGCCAGAAGC CATGCGGATC TGGATGGACG TTATCGTGAC TGGGGCAGAA
    GGCTACACTC ACTTCTTGTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87685
    Clone ID Related Accession (Same CDS sequence) XM_015471035.1 , XM_015471036.1 , XM_015471035.2
    Accession Version XM_015471035.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4104bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471035.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT
    GTAAATGAAA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTC
    CCTGCTGATG CTGATGCTAC TGCCGCTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA
    CAAAGTAAAG AGCAAAGATC GTCTGTCTGT CCTGGATTTA TGTTTCCTTC CACTCTTTCT
    TCTCATCCTA TCACTCAACC TCCATCAGCC CCTTGGCCTG AAGCCGTGGC TACCTTTGTC
    GATTCTTTTC CTTTAAATGA CACACCTCCT CCTCTACCAG CTAAGAAACA CAGGAGGCAA
    CAGCAGCAGG ACCAGCAGCA TTTTAGAAGT CTGGAAGAAA GGAAAAAACA GCACCAGGAA
    GGCCTCTATC TGAGTGACAC TTTGCCTCGA AAGAAAACCA CACCTTCCAT ATCCCCACAT
    TTCAGCAGTG CTACTATGGG GAGAAGCACC ACCCCAAAGG GCCACCTGCC CCTGGGACAG
    AGCAACAGCT GTGGCAGCGT GCTCCCCCAC TCGCTAGCAA CCATGACCAA AGACTCAGAA
    TCTCGGAGGA TGCTCAGAGG AAAGTCCAAG AAAGGAAGAA TGAATTTCTC AGCAGGAGCA
    GTTAGTAATT CCAAAAGGTC AAGTGTCAAA GGATATAATC ACCAACAGAT GAGTGAAGGA
    CAAAGGCAGA AGTCTTCTGA ATTTTACAAC CGCACAGCAT CTGAATCGAA TGTCTACTTG
    AACAGTTTCC ACTACCCAGA TCACAGCTAC AAGGACCAGG CCTTTGATAC TCTGAGCCTA
    GACAGCTCTG ACAGCATGGA GACCAGCATC TCTGCCTGCT CACCTGACAA TATCTCTAGT
    GCCAGCACTT CAAATATTGC CCGAATAGAG GAAATGGAGA GACTGTTGAA GCAGGCTCAT
    GCAGAGAAGA CAAGGCTGCT TGAATCCAGG GAACGAGAAA TGGAAGCCAA GAAACGCGCT
    CTGGAAGAAG AAAAACGACG TCGAGAACTC CTGGAAAAAC GGTTGCAAGA AGAAACTAGC
    CAGAGGCAGA AGTTAATTGA AAAGGAAGTA AAAATAAGGG AGAAACAAAG GGCACAGGCT
    CGACCTCTGA CCCGCTATCT GCCTGTGCGG AAGGAAGACT TTGATTTGCG GAGCCACGTA
    GAGACTGCTG GCCACAATAT CGATACGTGT TATCATGTGT CAATCACAGA GAAGACCTGC
    AGAGGATTCC TCATCAAAAT GGGTGGAAAA ATTAAAACCT GGAAAAAACG TTGGTTTGTT
    TTTGATCGGA ACAAGCGCAC CTTCTCTTAT TATGCAGACA AGCATGAAGC TAAATTAAAA
    GGAGTAATAT ACTTTCAAGC CATTGAAGAA GTATATTATG ATCACCTCAA GAACGCTAAT
    AAGAGTCCTA ATCCTTTACT TACCTTTAGC GTCAAGACAC ATGACAGAAT CTACTACATG
    GTGGCCCCGT CGCCAGAAGC CATGCGGATC TGGATGGACG TTATCGTGAC TGGGGCAGAA
    GGCTACACTC ACTTCTTGTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326521.1
    CDS283..4386
    Translation

    Target ORF information:

    RefSeq Version XM_015471035.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471035.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT
    GTAAATGAAA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTC
    CCTGCTGATG CTGATGCTAC TGCCGCTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA
    CAAAGTAAAG AGCAAAGATC GTCTGTCTGT CCTGGATTTA TGTTTCCTTC CACTCTTTCT
    TCTCATCCTA TCACTCAACC TCCATCAGCC CCTTGGCCTG AAGCCGTGGC TACCTTTGTC
    GATTCTTTTC CTTTAAATGA CACACCTCCT CCTCTACCAG CTAAGAAACA CAGGAGGCAA
    CAGCAGCAGG ACCAGCAGCA TTTTAGAAGT CTGGAAGAAA GGAAAAAACA GCACCAGGAA
    GGCCTCTATC TGAGTGACAC TTTGCCTCGA AAGAAAACCA CACCTTCCAT ATCCCCACAT
    TTCAGCAGTG CTACTATGGG GAGAAGCACC ACCCCAAAGG GCCACCTGCC CCTGGGACAG
    AGCAACAGCT GTGGCAGCGT GCTCCCCCAC TCGCTAGCAA CCATGACCAA AGACTCAGAA
    TCTCGGAGGA TGCTCAGAGG AAAGTCCAAG AAAGGAAGAA TGAATTTCTC AGCAGGAGCA
    GTTAGTAATT CCAAAAGGTC AAGTGTCAAA GGATATAATC ACCAACAGAT GAGTGAAGGA
    CAAAGGCAGA AGTCTTCTGA ATTTTACAAC CGCACAGCAT CTGAATCGAA TGTCTACTTG
    AACAGTTTCC ACTACCCAGA TCACAGCTAC AAGGACCAGG CCTTTGATAC TCTGAGCCTA
    GACAGCTCTG ACAGCATGGA GACCAGCATC TCTGCCTGCT CACCTGACAA TATCTCTAGT
    GCCAGCACTT CAAATATTGC CCGAATAGAG GAAATGGAGA GACTGTTGAA GCAGGCTCAT
    GCAGAGAAGA CAAGGCTGCT TGAATCCAGG GAACGAGAAA TGGAAGCCAA GAAACGCGCT
    CTGGAAGAAG AAAAACGACG TCGAGAACTC CTGGAAAAAC GGTTGCAAGA AGAAACTAGC
    CAGAGGCAGA AGTTAATTGA AAAGGAAGTA AAAATAAGGG AGAAACAAAG GGCACAGGCT
    CGACCTCTGA CCCGCTATCT GCCTGTGCGG AAGGAAGACT TTGATTTGCG GAGCCACGTA
    GAGACTGCTG GCCACAATAT CGATACGTGT TATCATGTGT CAATCACAGA GAAGACCTGC
    AGAGGATTCC TCATCAAAAT GGGTGGAAAA ATTAAAACCT GGAAAAAACG TTGGTTTGTT
    TTTGATCGGA ACAAGCGCAC CTTCTCTTAT TATGCAGACA AGCATGAAGC TAAATTAAAA
    GGAGTAATAT ACTTTCAAGC CATTGAAGAA GTATATTATG ATCACCTCAA GAACGCTAAT
    AAGAGTCCTA ATCCTTTACT TACCTTTAGC GTCAAGACAC ATGACAGAAT CTACTACATG
    GTGGCCCCGT CGCCAGAAGC CATGCGGATC TGGATGGACG TTATCGTGAC TGGGGCAGAA
    GGCTACACTC ACTTCTTGTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87686
    Clone ID Related Accession (Same CDS sequence) XM_015471038.1 , XM_015471038.2 , XM_015471039.2 , XM_015471041.1 , XM_015471039.1
    Accession Version XM_015471038.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4023bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103810.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCAGAGC AGAGCCACAT GCAAAAGCAG CTGGATTTAC AGAATGGTAG CTTAGAGGCA 
    GGCTTTGCGG AGAACTCCCT CGAAAACGAT TCCCAAAACA TGATGGAGAG CCTGAGCCCA
    AAGAAATATT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTGTTCTGG CTCCTATTTA
    ACCCTCTCAC AACCTGTGCC TGCTAAAAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA
    AGTAGCCCTT CTTTGGCCAA AATTCAGGGA ACGAAGCAGT TCTCTTGTGA TGGAAGTGAG
    AAAAGTATTT CCATGAAACC GCCCACCCCT TTACGCAGCA CCTCACCCTC CCTTAGTGGA
    TATGCACTTG GGAGAGCAGA CTTTGATCAT TTTCTTGGCC GGGACGCCGA AAGGTCCTTG
    AGGCTTTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGAATAAATC CCATGACAAT
    GTCTACTTTC TCGGAGGGCT GGAAGGCCGA AAAGCATCTG GCTCGCTCCT GACCATGTGG
    AATGGAAGTT CCCTGAGTGA CACTGGCTCC TCACCCATCA ACAGGTCAGG GGCGGCCAGC
    ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATAAGCATCC AGGACAACCT GACACTTCAG
    CCCAAGTTGA GCAGACACCG GGACCCGGCA TCGGAAAACA TCAGTTTAAG AACCAGGAAG
    TACTCTAGCG GCAGCCTGAG TCACATGGGC GCCTACAGCC GCTCTCTCCC CAGGTTGCAT
    AGAGCCACCG AGAACCAGTT GGCACCCCTC AGTCTGCCTC CAAGAAGCTC TCTGGGCAAT
    TCCAAACGAA CGAAACTGGG GGAAAAGGAT CTACCTCATT GCATTATAGA CAATGACAAT
    TACCTGAATT TTTCTTCTTT GAGCTCAGGA GCTTTGCCCT ATAAAGCCTC TGCCTCCGAG
    GGCAGTGCGT ACGTGAGTTC CACCCTCAGC GTGCCTGCCA GTCCTCGAGT GGCTCGCAAG
    ATGCTTCTGG CCTCCACCTC CTCCTGCACC TCTGACGACC TTGACAGGGC TTCCTACTCG
    GGTACCAGCC CTGGTCATTC GTTTCCTCCT GGGGAGCTGG ACCGAGCATT TGCAGCCAGA
    AGGAACTTCT CGTGTGGATC TGTCGAGTTT GATGATGCAG ACTTGGACAG CCTCAGACAG
    GCCTCAGGAA CCCCCCAGCC TGTCCTGCGG GAACGGAAAA GCAGCATTAG CTCCATTTCC
    GGACGAGACG ACCTGATGGA TTATCATCGG CGGCAGAGGG AGGAGAGACT CAGGGAGCAG
    GAGATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGCCTCTG TGCTGAGTAC
    ACAAAGCCTG ACAGTCGTGT GTCCCCCGGC ACCACCGTGG CAGATGTGCA GAAAATCAAT
    AAAGAGCTGG AGAAACTGCA GCTCTCGGAT GAGGAGTCTG TGTTTGAAGA AGCCCTGGTG
    AGCCCGGACA CCAGGTACAG GTGCCACCAG AAAAGCGCTC TCCACGACGC GGACCTGGCA
    GGCTTTGGGA GCCTCAGTCA GAGCAGTGCC AGCTTCCTCC CGCCACGGGG CGCCAGGAAT
    GATGAACTTC TCGGGGACCT CGCCAGGACT CCCCCGCCGC CCTCCTCTGC CTTTCTGAAA
    GCTTCCGGCG AGTCCGCTTA CCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA
    GAACAAAGGA TTCAGGAGTT GACTGCGATG GAAGAGACCC GGATAGCAAT TCTGAACAAC
    CTTGAGGAAC TTGAACAAAA AATCAAAGAT ATAAATGACC AGATGGAGGA ATCTTCCAGA
    GAGCTGGATA TGGAATGTGC TCTCTTGGAT GGAGAACAAA AATCAGAAAC AACTGAACTT
    ATGAAAGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TCACAGAACT GGAAAAGAAC
    ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG
    AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG
    AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCCGACC TACTGGATGT TGAGAGCAAA
    CACTTTGAAG ACCTGGAATT CCAGCAGCTT GAGCGTGAAA GCCGTCTAGA TGAGGAAAAG
    GAGAACCTGA CCCAGCAACT CCTGCGTGAA GTGGCTGAGT ATCAACGGAA CATTGTTACT
    AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCTAATC ACATTGTCCA GCAGGCTCAA
    AGAGAACAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGCT GCAGAGAGAA
    AAGGAAAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT
    CCTGTCACCC CCAATACTCT GAAAGAGGGC TATATCATTG TAAATGAAAT TAATGAGTCA
    TGTGGCAGTT CCACGAATCT CCCCCCTTCC ACTCAGTTCC CTGCTGATGC TGATGCTACT
    GCCGCTGGGC CTACCACAGC TGTGCTGATG AGCCAGCCAC AAAGTAAAGA GCAAAGATCG
    TCTGTCTGTC CTGGATTTAT GTTTCCTTCC ACTCTTTCTT CTCATCCTAT CACTCAACCT
    CCATCAGCCC CTTGGCCTGA AGCCGTGGCT ACCTTTGTCG ATTCTTTTCC TTTAAATGAC
    ACACCTCCTC CTCTACCAGC TAAGAAACAC AGGAGGCAAC AGCAGCAGGA CCAGCAGCAT
    TTTAGAAGTC TGGAAGAAAG GAAAAAACAG CACCAGGAAG GCCTCTATCT GAGTGACACT
    TTGCCTCGAA AGAAAACCAC ACCTTCCATA TCCCCACATT TCAGCAGTGC TACTATGGGG
    AGAAGCACCA CCCCAAAGGG CCACCTGCCC CTGGGACAGA GCAACAGCTG TGGCAGCGTG
    CTCCCCCACT CGCTAGCAAC CATGACCAAA GACTCAGAAT CTCGGAGGAT GCTCAGAGGA
    AAGTCCAAGA AAGGAAGAAT GAATTTCTCA GCAGGAGCAG TTAGTAATTC CAAAAGGTCA
    AGTGTCAAAG GATATAATCA CCAACAGATG AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA
    TTTTACAACC GCACAGCATC TGAATCGAAT GTCTACTTGA ACAGTTTCCA CTACCCAGAT
    CACAGCTACA AGGACCAGGC CTTTGATACT CTGAGCCTAG ACAGCTCTGA CAGCATGGAG
    ACCAGCATCT CTGCCTGCTC ACCTGACAAT ATCTCTAGTG CCAGCACTTC AAATATTGCC
    CGAATAGAGG AAATGGAGAG ACTGTTGAAG CAGGCTCATG CAGAGAAGAC AAGGCTGCTT
    GAATCCAGGG AACGAGAAAT GGAAGCCAAG AAACGCGCTC TGGAAGAAGA AAAACGACGT
    CGAGAACTCC TGGAAAAACG GTTGCAAGAA GAAACTAGCC AGAGGCAGAA GTTAATTGAA
    AAGGAAGTAA AAATAAGGGA GAAACAAAGG GCACAGGCTC GACCTCTGAC CCGCTATCTG
    CCTGTGCGGA AGGAAGACTT TGATTTGCGG AGCCACGTAG AGACTGCTGG CCACAATATC
    GATACGTGTT ATCATGTGTC AATCACAGAG AAGACCTGCA GAGGATTCCT CATCAAAATG
    GGTGGAAAAA TTAAAACCTG GAAAAAACGT TGGTTTGTTT TTGATCGGAA CAAGCGCACC
    TTCTCTTATT ATGCAGACAA GCATGAAGCT AAATTAAAAG GAGTAATATA CTTTCAAGCC
    ATTGAAGAAG TATATTATGA TCACCTCAAG AACGCTAATA AGAGTCCTAA TCCTTTACTT
    ACCTTTAGCG TCAAGACACA TGACAGAATC TACTACATGG TGGCCCCGTC GCCAGAAGCC
    ATGCGGATCT GGATGGACGT TATCGTGACT GGGGCAGAAG GCTACACTCA CTTCTTGTTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326524.1
    CDS74..4096
    Translation

    Target ORF information:

    RefSeq Version XM_015471038.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471038.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCAGAGC AGAGCCACAT GCAAAAGCAG CTGGATTTAC AGAATGGTAG CTTAGAGGCA 
    GGCTTTGCGG AGAACTCCCT CGAAAACGAT TCCCAAAACA TGATGGAGAG CCTGAGCCCA
    AAGAAATATT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTGTTCTGG CTCCTATTTA
    ACCCTCTCAC AACCTGTGCC TGCTAAAAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA
    AGTAGCCCTT CTTTGGCCAA AATTCAGGGA ACGAAGCAGT TCTCTTGTGA TGGAAGTGAG
    AAAAGTATTT CCATGAAACC GCCCACCCCT TTACGCAGCA CCTCACCCTC CCTTAGTGGA
    TATGCACTTG GGAGAGCAGA CTTTGATCAT TTTCTTGGCC GGGACGCCGA AAGGTCCTTG
    AGGCTTTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGAATAAATC CCATGACAAT
    GTCTACTTTC TCGGAGGGCT GGAAGGCCGA AAAGCATCTG GCTCGCTCCT GACCATGTGG
    AATGGAAGTT CCCTGAGTGA CACTGGCTCC TCACCCATCA ACAGGTCAGG GGCGGCCAGC
    ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATAAGCATCC AGGACAACCT GACACTTCAG
    CCCAAGTTGA GCAGACACCG GGACCCGGCA TCGGAAAACA TCAGTTTAAG AACCAGGAAG
    TACTCTAGCG GCAGCCTGAG TCACATGGGC GCCTACAGCC GCTCTCTCCC CAGGTTGCAT
    AGAGCCACCG AGAACCAGTT GGCACCCCTC AGTCTGCCTC CAAGAAGCTC TCTGGGCAAT
    TCCAAACGAA CGAAACTGGG GGAAAAGGAT CTACCTCATT GCATTATAGA CAATGACAAT
    TACCTGAATT TTTCTTCTTT GAGCTCAGGA GCTTTGCCCT ATAAAGCCTC TGCCTCCGAG
    GGCAGTGCGT ACGTGAGTTC CACCCTCAGC GTGCCTGCCA GTCCTCGAGT GGCTCGCAAG
    ATGCTTCTGG CCTCCACCTC CTCCTGCACC TCTGACGACC TTGACAGGGC TTCCTACTCG
    GGTACCAGCC CTGGTCATTC GTTTCCTCCT GGGGAGCTGG ACCGAGCATT TGCAGCCAGA
    AGGAACTTCT CGTGTGGATC TGTCGAGTTT GATGATGCAG ACTTGGACAG CCTCAGACAG
    GCCTCAGGAA CCCCCCAGCC TGTCCTGCGG GAACGGAAAA GCAGCATTAG CTCCATTTCC
    GGACGAGACG ACCTGATGGA TTATCATCGG CGGCAGAGGG AGGAGAGACT CAGGGAGCAG
    GAGATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGCCTCTG TGCTGAGTAC
    ACAAAGCCTG ACAGTCGTGT GTCCCCCGGC ACCACCGTGG CAGATGTGCA GAAAATCAAT
    AAAGAGCTGG AGAAACTGCA GCTCTCGGAT GAGGAGTCTG TGTTTGAAGA AGCCCTGGTG
    AGCCCGGACA CCAGGTACAG GTGCCACCAG AAAAGCGCTC TCCACGACGC GGACCTGGCA
    GGCTTTGGGA GCCTCAGTCA GAGCAGTGCC AGCTTCCTCC CGCCACGGGG CGCCAGGAAT
    GATGAACTTC TCGGGGACCT CGCCAGGACT CCCCCGCCGC CCTCCTCTGC CTTTCTGAAA
    GCTTCCGGCG AGTCCGCTTA CCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA
    GAACAAAGGA TTCAGGAGTT GACTGCGATG GAAGAGACCC GGATAGCAAT TCTGAACAAC
    CTTGAGGAAC TTGAACAAAA AATCAAAGAT ATAAATGACC AGATGGAGGA ATCTTCCAGA
    GAGCTGGATA TGGAATGTGC TCTCTTGGAT GGAGAACAAA AATCAGAAAC AACTGAACTT
    ATGAAAGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TCACAGAACT GGAAAAGAAC
    ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG
    AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG
    AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCCGACC TACTGGATGT TGAGAGCAAA
    CACTTTGAAG ACCTGGAATT CCAGCAGCTT GAGCGTGAAA GCCGTCTAGA TGAGGAAAAG
    GAGAACCTGA CCCAGCAACT CCTGCGTGAA GTGGCTGAGT ATCAACGGAA CATTGTTACT
    AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCTAATC ACATTGTCCA GCAGGCTCAA
    AGAGAACAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGCT GCAGAGAGAA
    AAGGAAAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT
    CCTGTCACCC CCAATACTCT GAAAGAGGGC TATATCATTG TAAATGAAAT TAATGAGTCA
    TGTGGCAGTT CCACGAATCT CCCCCCTTCC ACTCAGTTCC CTGCTGATGC TGATGCTACT
    GCCGCTGGGC CTACCACAGC TGTGCTGATG AGCCAGCCAC AAAGTAAAGA GCAAAGATCG
    TCTGTCTGTC CTGGATTTAT GTTTCCTTCC ACTCTTTCTT CTCATCCTAT CACTCAACCT
    CCATCAGCCC CTTGGCCTGA AGCCGTGGCT ACCTTTGTCG ATTCTTTTCC TTTAAATGAC
    ACACCTCCTC CTCTACCAGC TAAGAAACAC AGGAGGCAAC AGCAGCAGGA CCAGCAGCAT
    TTTAGAAGTC TGGAAGAAAG GAAAAAACAG CACCAGGAAG GCCTCTATCT GAGTGACACT
    TTGCCTCGAA AGAAAACCAC ACCTTCCATA TCCCCACATT TCAGCAGTGC TACTATGGGG
    AGAAGCACCA CCCCAAAGGG CCACCTGCCC CTGGGACAGA GCAACAGCTG TGGCAGCGTG
    CTCCCCCACT CGCTAGCAAC CATGACCAAA GACTCAGAAT CTCGGAGGAT GCTCAGAGGA
    AAGTCCAAGA AAGGAAGAAT GAATTTCTCA GCAGGAGCAG TTAGTAATTC CAAAAGGTCA
    AGTGTCAAAG GATATAATCA CCAACAGATG AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA
    TTTTACAACC GCACAGCATC TGAATCGAAT GTCTACTTGA ACAGTTTCCA CTACCCAGAT
    CACAGCTACA AGGACCAGGC CTTTGATACT CTGAGCCTAG ACAGCTCTGA CAGCATGGAG
    ACCAGCATCT CTGCCTGCTC ACCTGACAAT ATCTCTAGTG CCAGCACTTC AAATATTGCC
    CGAATAGAGG AAATGGAGAG ACTGTTGAAG CAGGCTCATG CAGAGAAGAC AAGGCTGCTT
    GAATCCAGGG AACGAGAAAT GGAAGCCAAG AAACGCGCTC TGGAAGAAGA AAAACGACGT
    CGAGAACTCC TGGAAAAACG GTTGCAAGAA GAAACTAGCC AGAGGCAGAA GTTAATTGAA
    AAGGAAGTAA AAATAAGGGA GAAACAAAGG GCACAGGCTC GACCTCTGAC CCGCTATCTG
    CCTGTGCGGA AGGAAGACTT TGATTTGCGG AGCCACGTAG AGACTGCTGG CCACAATATC
    GATACGTGTT ATCATGTGTC AATCACAGAG AAGACCTGCA GAGGATTCCT CATCAAAATG
    GGTGGAAAAA TTAAAACCTG GAAAAAACGT TGGTTTGTTT TTGATCGGAA CAAGCGCACC
    TTCTCTTATT ATGCAGACAA GCATGAAGCT AAATTAAAAG GAGTAATATA CTTTCAAGCC
    ATTGAAGAAG TATATTATGA TCACCTCAAG AACGCTAATA AGAGTCCTAA TCCTTTACTT
    ACCTTTAGCG TCAAGACACA TGACAGAATC TACTACATGG TGGCCCCGTC GCCAGAAGCC
    ATGCGGATCT GGATGGACGT TATCGTGACT GGGGCAGAAG GCTACACTCA CTTCTTGTTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87686
    Clone ID Related Accession (Same CDS sequence) XM_015471038.1 , XM_015471038.2 , XM_015471039.2 , XM_015471041.1 , XM_015471039.1
    Accession Version XM_015471038.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4023bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471038.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCAGAGC AGAGCCACAT GCAAAAGCAG CTGGATTTAC AGAATGGTAG CTTAGAGGCA 
    GGCTTTGCGG AGAACTCCCT CGAAAACGAT TCCCAAAACA TGATGGAGAG CCTGAGCCCA
    AAGAAATATT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTGTTCTGG CTCCTATTTA
    ACCCTCTCAC AACCTGTGCC TGCTAAAAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA
    AGTAGCCCTT CTTTGGCCAA AATTCAGGGA ACGAAGCAGT TCTCTTGTGA TGGAAGTGAG
    AAAAGTATTT CCATGAAACC GCCCACCCCT TTACGCAGCA CCTCACCCTC CCTTAGTGGA
    TATGCACTTG GGAGAGCAGA CTTTGATCAT TTTCTTGGCC GGGACGCCGA AAGGTCCTTG
    AGGCTTTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGAATAAATC CCATGACAAT
    GTCTACTTTC TCGGAGGGCT GGAAGGCCGA AAAGCATCTG GCTCGCTCCT GACCATGTGG
    AATGGAAGTT CCCTGAGTGA CACTGGCTCC TCACCCATCA ACAGGTCAGG GGCGGCCAGC
    ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATAAGCATCC AGGACAACCT GACACTTCAG
    CCCAAGTTGA GCAGACACCG GGACCCGGCA TCGGAAAACA TCAGTTTAAG AACCAGGAAG
    TACTCTAGCG GCAGCCTGAG TCACATGGGC GCCTACAGCC GCTCTCTCCC CAGGTTGCAT
    AGAGCCACCG AGAACCAGTT GGCACCCCTC AGTCTGCCTC CAAGAAGCTC TCTGGGCAAT
    TCCAAACGAA CGAAACTGGG GGAAAAGGAT CTACCTCATT GCATTATAGA CAATGACAAT
    TACCTGAATT TTTCTTCTTT GAGCTCAGGA GCTTTGCCCT ATAAAGCCTC TGCCTCCGAG
    GGCAGTGCGT ACGTGAGTTC CACCCTCAGC GTGCCTGCCA GTCCTCGAGT GGCTCGCAAG
    ATGCTTCTGG CCTCCACCTC CTCCTGCACC TCTGACGACC TTGACAGGGC TTCCTACTCG
    GGTACCAGCC CTGGTCATTC GTTTCCTCCT GGGGAGCTGG ACCGAGCATT TGCAGCCAGA
    AGGAACTTCT CGTGTGGATC TGTCGAGTTT GATGATGCAG ACTTGGACAG CCTCAGACAG
    GCCTCAGGAA CCCCCCAGCC TGTCCTGCGG GAACGGAAAA GCAGCATTAG CTCCATTTCC
    GGACGAGACG ACCTGATGGA TTATCATCGG CGGCAGAGGG AGGAGAGACT CAGGGAGCAG
    GAGATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGCCTCTG TGCTGAGTAC
    ACAAAGCCTG ACAGTCGTGT GTCCCCCGGC ACCACCGTGG CAGATGTGCA GAAAATCAAT
    AAAGAGCTGG AGAAACTGCA GCTCTCGGAT GAGGAGTCTG TGTTTGAAGA AGCCCTGGTG
    AGCCCGGACA CCAGGTACAG GTGCCACCAG AAAAGCGCTC TCCACGACGC GGACCTGGCA
    GGCTTTGGGA GCCTCAGTCA GAGCAGTGCC AGCTTCCTCC CGCCACGGGG CGCCAGGAAT
    GATGAACTTC TCGGGGACCT CGCCAGGACT CCCCCGCCGC CCTCCTCTGC CTTTCTGAAA
    GCTTCCGGCG AGTCCGCTTA CCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA
    GAACAAAGGA TTCAGGAGTT GACTGCGATG GAAGAGACCC GGATAGCAAT TCTGAACAAC
    CTTGAGGAAC TTGAACAAAA AATCAAAGAT ATAAATGACC AGATGGAGGA ATCTTCCAGA
    GAGCTGGATA TGGAATGTGC TCTCTTGGAT GGAGAACAAA AATCAGAAAC AACTGAACTT
    ATGAAAGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TCACAGAACT GGAAAAGAAC
    ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG
    AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG
    AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCCGACC TACTGGATGT TGAGAGCAAA
    CACTTTGAAG ACCTGGAATT CCAGCAGCTT GAGCGTGAAA GCCGTCTAGA TGAGGAAAAG
    GAGAACCTGA CCCAGCAACT CCTGCGTGAA GTGGCTGAGT ATCAACGGAA CATTGTTACT
    AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCTAATC ACATTGTCCA GCAGGCTCAA
    AGAGAACAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGCT GCAGAGAGAA
    AAGGAAAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT
    CCTGTCACCC CCAATACTCT GAAAGAGGGC TATATCATTG TAAATGAAAT TAATGAGTCA
    TGTGGCAGTT CCACGAATCT CCCCCCTTCC ACTCAGTTCC CTGCTGATGC TGATGCTACT
    GCCGCTGGGC CTACCACAGC TGTGCTGATG AGCCAGCCAC AAAGTAAAGA GCAAAGATCG
    TCTGTCTGTC CTGGATTTAT GTTTCCTTCC ACTCTTTCTT CTCATCCTAT CACTCAACCT
    CCATCAGCCC CTTGGCCTGA AGCCGTGGCT ACCTTTGTCG ATTCTTTTCC TTTAAATGAC
    ACACCTCCTC CTCTACCAGC TAAGAAACAC AGGAGGCAAC AGCAGCAGGA CCAGCAGCAT
    TTTAGAAGTC TGGAAGAAAG GAAAAAACAG CACCAGGAAG GCCTCTATCT GAGTGACACT
    TTGCCTCGAA AGAAAACCAC ACCTTCCATA TCCCCACATT TCAGCAGTGC TACTATGGGG
    AGAAGCACCA CCCCAAAGGG CCACCTGCCC CTGGGACAGA GCAACAGCTG TGGCAGCGTG
    CTCCCCCACT CGCTAGCAAC CATGACCAAA GACTCAGAAT CTCGGAGGAT GCTCAGAGGA
    AAGTCCAAGA AAGGAAGAAT GAATTTCTCA GCAGGAGCAG TTAGTAATTC CAAAAGGTCA
    AGTGTCAAAG GATATAATCA CCAACAGATG AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA
    TTTTACAACC GCACAGCATC TGAATCGAAT GTCTACTTGA ACAGTTTCCA CTACCCAGAT
    CACAGCTACA AGGACCAGGC CTTTGATACT CTGAGCCTAG ACAGCTCTGA CAGCATGGAG
    ACCAGCATCT CTGCCTGCTC ACCTGACAAT ATCTCTAGTG CCAGCACTTC AAATATTGCC
    CGAATAGAGG AAATGGAGAG ACTGTTGAAG CAGGCTCATG CAGAGAAGAC AAGGCTGCTT
    GAATCCAGGG AACGAGAAAT GGAAGCCAAG AAACGCGCTC TGGAAGAAGA AAAACGACGT
    CGAGAACTCC TGGAAAAACG GTTGCAAGAA GAAACTAGCC AGAGGCAGAA GTTAATTGAA
    AAGGAAGTAA AAATAAGGGA GAAACAAAGG GCACAGGCTC GACCTCTGAC CCGCTATCTG
    CCTGTGCGGA AGGAAGACTT TGATTTGCGG AGCCACGTAG AGACTGCTGG CCACAATATC
    GATACGTGTT ATCATGTGTC AATCACAGAG AAGACCTGCA GAGGATTCCT CATCAAAATG
    GGTGGAAAAA TTAAAACCTG GAAAAAACGT TGGTTTGTTT TTGATCGGAA CAAGCGCACC
    TTCTCTTATT ATGCAGACAA GCATGAAGCT AAATTAAAAG GAGTAATATA CTTTCAAGCC
    ATTGAAGAAG TATATTATGA TCACCTCAAG AACGCTAATA AGAGTCCTAA TCCTTTACTT
    ACCTTTAGCG TCAAGACACA TGACAGAATC TACTACATGG TGGCCCCGTC GCCAGAAGCC
    ATGCGGATCT GGATGGACGT TATCGTGACT GGGGCAGAAG GCTACACTCA CTTCTTGTTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326524.1
    CDS71..4093
    Translation

    Target ORF information:

    RefSeq Version XM_015471038.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471038.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCAGAGC AGAGCCACAT GCAAAAGCAG CTGGATTTAC AGAATGGTAG CTTAGAGGCA 
    GGCTTTGCGG AGAACTCCCT CGAAAACGAT TCCCAAAACA TGATGGAGAG CCTGAGCCCA
    AAGAAATATT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTGTTCTGG CTCCTATTTA
    ACCCTCTCAC AACCTGTGCC TGCTAAAAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA
    AGTAGCCCTT CTTTGGCCAA AATTCAGGGA ACGAAGCAGT TCTCTTGTGA TGGAAGTGAG
    AAAAGTATTT CCATGAAACC GCCCACCCCT TTACGCAGCA CCTCACCCTC CCTTAGTGGA
    TATGCACTTG GGAGAGCAGA CTTTGATCAT TTTCTTGGCC GGGACGCCGA AAGGTCCTTG
    AGGCTTTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGAATAAATC CCATGACAAT
    GTCTACTTTC TCGGAGGGCT GGAAGGCCGA AAAGCATCTG GCTCGCTCCT GACCATGTGG
    AATGGAAGTT CCCTGAGTGA CACTGGCTCC TCACCCATCA ACAGGTCAGG GGCGGCCAGC
    ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATAAGCATCC AGGACAACCT GACACTTCAG
    CCCAAGTTGA GCAGACACCG GGACCCGGCA TCGGAAAACA TCAGTTTAAG AACCAGGAAG
    TACTCTAGCG GCAGCCTGAG TCACATGGGC GCCTACAGCC GCTCTCTCCC CAGGTTGCAT
    AGAGCCACCG AGAACCAGTT GGCACCCCTC AGTCTGCCTC CAAGAAGCTC TCTGGGCAAT
    TCCAAACGAA CGAAACTGGG GGAAAAGGAT CTACCTCATT GCATTATAGA CAATGACAAT
    TACCTGAATT TTTCTTCTTT GAGCTCAGGA GCTTTGCCCT ATAAAGCCTC TGCCTCCGAG
    GGCAGTGCGT ACGTGAGTTC CACCCTCAGC GTGCCTGCCA GTCCTCGAGT GGCTCGCAAG
    ATGCTTCTGG CCTCCACCTC CTCCTGCACC TCTGACGACC TTGACAGGGC TTCCTACTCG
    GGTACCAGCC CTGGTCATTC GTTTCCTCCT GGGGAGCTGG ACCGAGCATT TGCAGCCAGA
    AGGAACTTCT CGTGTGGATC TGTCGAGTTT GATGATGCAG ACTTGGACAG CCTCAGACAG
    GCCTCAGGAA CCCCCCAGCC TGTCCTGCGG GAACGGAAAA GCAGCATTAG CTCCATTTCC
    GGACGAGACG ACCTGATGGA TTATCATCGG CGGCAGAGGG AGGAGAGACT CAGGGAGCAG
    GAGATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGCCTCTG TGCTGAGTAC
    ACAAAGCCTG ACAGTCGTGT GTCCCCCGGC ACCACCGTGG CAGATGTGCA GAAAATCAAT
    AAAGAGCTGG AGAAACTGCA GCTCTCGGAT GAGGAGTCTG TGTTTGAAGA AGCCCTGGTG
    AGCCCGGACA CCAGGTACAG GTGCCACCAG AAAAGCGCTC TCCACGACGC GGACCTGGCA
    GGCTTTGGGA GCCTCAGTCA GAGCAGTGCC AGCTTCCTCC CGCCACGGGG CGCCAGGAAT
    GATGAACTTC TCGGGGACCT CGCCAGGACT CCCCCGCCGC CCTCCTCTGC CTTTCTGAAA
    GCTTCCGGCG AGTCCGCTTA CCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA
    GAACAAAGGA TTCAGGAGTT GACTGCGATG GAAGAGACCC GGATAGCAAT TCTGAACAAC
    CTTGAGGAAC TTGAACAAAA AATCAAAGAT ATAAATGACC AGATGGAGGA ATCTTCCAGA
    GAGCTGGATA TGGAATGTGC TCTCTTGGAT GGAGAACAAA AATCAGAAAC AACTGAACTT
    ATGAAAGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TCACAGAACT GGAAAAGAAC
    ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG
    AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG
    AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCCGACC TACTGGATGT TGAGAGCAAA
    CACTTTGAAG ACCTGGAATT CCAGCAGCTT GAGCGTGAAA GCCGTCTAGA TGAGGAAAAG
    GAGAACCTGA CCCAGCAACT CCTGCGTGAA GTGGCTGAGT ATCAACGGAA CATTGTTACT
    AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCTAATC ACATTGTCCA GCAGGCTCAA
    AGAGAACAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGCT GCAGAGAGAA
    AAGGAAAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT
    CCTGTCACCC CCAATACTCT GAAAGAGGGC TATATCATTG TAAATGAAAT TAATGAGTCA
    TGTGGCAGTT CCACGAATCT CCCCCCTTCC ACTCAGTTCC CTGCTGATGC TGATGCTACT
    GCCGCTGGGC CTACCACAGC TGTGCTGATG AGCCAGCCAC AAAGTAAAGA GCAAAGATCG
    TCTGTCTGTC CTGGATTTAT GTTTCCTTCC ACTCTTTCTT CTCATCCTAT CACTCAACCT
    CCATCAGCCC CTTGGCCTGA AGCCGTGGCT ACCTTTGTCG ATTCTTTTCC TTTAAATGAC
    ACACCTCCTC CTCTACCAGC TAAGAAACAC AGGAGGCAAC AGCAGCAGGA CCAGCAGCAT
    TTTAGAAGTC TGGAAGAAAG GAAAAAACAG CACCAGGAAG GCCTCTATCT GAGTGACACT
    TTGCCTCGAA AGAAAACCAC ACCTTCCATA TCCCCACATT TCAGCAGTGC TACTATGGGG
    AGAAGCACCA CCCCAAAGGG CCACCTGCCC CTGGGACAGA GCAACAGCTG TGGCAGCGTG
    CTCCCCCACT CGCTAGCAAC CATGACCAAA GACTCAGAAT CTCGGAGGAT GCTCAGAGGA
    AAGTCCAAGA AAGGAAGAAT GAATTTCTCA GCAGGAGCAG TTAGTAATTC CAAAAGGTCA
    AGTGTCAAAG GATATAATCA CCAACAGATG AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA
    TTTTACAACC GCACAGCATC TGAATCGAAT GTCTACTTGA ACAGTTTCCA CTACCCAGAT
    CACAGCTACA AGGACCAGGC CTTTGATACT CTGAGCCTAG ACAGCTCTGA CAGCATGGAG
    ACCAGCATCT CTGCCTGCTC ACCTGACAAT ATCTCTAGTG CCAGCACTTC AAATATTGCC
    CGAATAGAGG AAATGGAGAG ACTGTTGAAG CAGGCTCATG CAGAGAAGAC AAGGCTGCTT
    GAATCCAGGG AACGAGAAAT GGAAGCCAAG AAACGCGCTC TGGAAGAAGA AAAACGACGT
    CGAGAACTCC TGGAAAAACG GTTGCAAGAA GAAACTAGCC AGAGGCAGAA GTTAATTGAA
    AAGGAAGTAA AAATAAGGGA GAAACAAAGG GCACAGGCTC GACCTCTGAC CCGCTATCTG
    CCTGTGCGGA AGGAAGACTT TGATTTGCGG AGCCACGTAG AGACTGCTGG CCACAATATC
    GATACGTGTT ATCATGTGTC AATCACAGAG AAGACCTGCA GAGGATTCCT CATCAAAATG
    GGTGGAAAAA TTAAAACCTG GAAAAAACGT TGGTTTGTTT TTGATCGGAA CAAGCGCACC
    TTCTCTTATT ATGCAGACAA GCATGAAGCT AAATTAAAAG GAGTAATATA CTTTCAAGCC
    ATTGAAGAAG TATATTATGA TCACCTCAAG AACGCTAATA AGAGTCCTAA TCCTTTACTT
    ACCTTTAGCG TCAAGACACA TGACAGAATC TACTACATGG TGGCCCCGTC GCCAGAAGCC
    ATGCGGATCT GGATGGACGT TATCGTGACT GGGGCAGAAG GCTACACTCA CTTCTTGTTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87686
    Clone ID Related Accession (Same CDS sequence) XM_015471038.1 , XM_015471038.2 , XM_015471039.2 , XM_015471041.1 , XM_015471039.1
    Accession Version XM_015471039.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4023bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471039.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCAGAGC AGAGCCACAT GCAAAAGCAG CTGGATTTAC AGAATGGTAG CTTAGAGGCA 
    GGCTTTGCGG AGAACTCCCT CGAAAACGAT TCCCAAAACA TGATGGAGAG CCTGAGCCCA
    AAGAAATATT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTGTTCTGG CTCCTATTTA
    ACCCTCTCAC AACCTGTGCC TGCTAAAAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA
    AGTAGCCCTT CTTTGGCCAA AATTCAGGGA ACGAAGCAGT TCTCTTGTGA TGGAAGTGAG
    AAAAGTATTT CCATGAAACC GCCCACCCCT TTACGCAGCA CCTCACCCTC CCTTAGTGGA
    TATGCACTTG GGAGAGCAGA CTTTGATCAT TTTCTTGGCC GGGACGCCGA AAGGTCCTTG
    AGGCTTTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGAATAAATC CCATGACAAT
    GTCTACTTTC TCGGAGGGCT GGAAGGCCGA AAAGCATCTG GCTCGCTCCT GACCATGTGG
    AATGGAAGTT CCCTGAGTGA CACTGGCTCC TCACCCATCA ACAGGTCAGG GGCGGCCAGC
    ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATAAGCATCC AGGACAACCT GACACTTCAG
    CCCAAGTTGA GCAGACACCG GGACCCGGCA TCGGAAAACA TCAGTTTAAG AACCAGGAAG
    TACTCTAGCG GCAGCCTGAG TCACATGGGC GCCTACAGCC GCTCTCTCCC CAGGTTGCAT
    AGAGCCACCG AGAACCAGTT GGCACCCCTC AGTCTGCCTC CAAGAAGCTC TCTGGGCAAT
    TCCAAACGAA CGAAACTGGG GGAAAAGGAT CTACCTCATT GCATTATAGA CAATGACAAT
    TACCTGAATT TTTCTTCTTT GAGCTCAGGA GCTTTGCCCT ATAAAGCCTC TGCCTCCGAG
    GGCAGTGCGT ACGTGAGTTC CACCCTCAGC GTGCCTGCCA GTCCTCGAGT GGCTCGCAAG
    ATGCTTCTGG CCTCCACCTC CTCCTGCACC TCTGACGACC TTGACAGGGC TTCCTACTCG
    GGTACCAGCC CTGGTCATTC GTTTCCTCCT GGGGAGCTGG ACCGAGCATT TGCAGCCAGA
    AGGAACTTCT CGTGTGGATC TGTCGAGTTT GATGATGCAG ACTTGGACAG CCTCAGACAG
    GCCTCAGGAA CCCCCCAGCC TGTCCTGCGG GAACGGAAAA GCAGCATTAG CTCCATTTCC
    GGACGAGACG ACCTGATGGA TTATCATCGG CGGCAGAGGG AGGAGAGACT CAGGGAGCAG
    GAGATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGCCTCTG TGCTGAGTAC
    ACAAAGCCTG ACAGTCGTGT GTCCCCCGGC ACCACCGTGG CAGATGTGCA GAAAATCAAT
    AAAGAGCTGG AGAAACTGCA GCTCTCGGAT GAGGAGTCTG TGTTTGAAGA AGCCCTGGTG
    AGCCCGGACA CCAGGTACAG GTGCCACCAG AAAAGCGCTC TCCACGACGC GGACCTGGCA
    GGCTTTGGGA GCCTCAGTCA GAGCAGTGCC AGCTTCCTCC CGCCACGGGG CGCCAGGAAT
    GATGAACTTC TCGGGGACCT CGCCAGGACT CCCCCGCCGC CCTCCTCTGC CTTTCTGAAA
    GCTTCCGGCG AGTCCGCTTA CCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA
    GAACAAAGGA TTCAGGAGTT GACTGCGATG GAAGAGACCC GGATAGCAAT TCTGAACAAC
    CTTGAGGAAC TTGAACAAAA AATCAAAGAT ATAAATGACC AGATGGAGGA ATCTTCCAGA
    GAGCTGGATA TGGAATGTGC TCTCTTGGAT GGAGAACAAA AATCAGAAAC AACTGAACTT
    ATGAAAGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TCACAGAACT GGAAAAGAAC
    ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG
    AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG
    AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCCGACC TACTGGATGT TGAGAGCAAA
    CACTTTGAAG ACCTGGAATT CCAGCAGCTT GAGCGTGAAA GCCGTCTAGA TGAGGAAAAG
    GAGAACCTGA CCCAGCAACT CCTGCGTGAA GTGGCTGAGT ATCAACGGAA CATTGTTACT
    AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCTAATC ACATTGTCCA GCAGGCTCAA
    AGAGAACAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGCT GCAGAGAGAA
    AAGGAAAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT
    CCTGTCACCC CCAATACTCT GAAAGAGGGC TATATCATTG TAAATGAAAT TAATGAGTCA
    TGTGGCAGTT CCACGAATCT CCCCCCTTCC ACTCAGTTCC CTGCTGATGC TGATGCTACT
    GCCGCTGGGC CTACCACAGC TGTGCTGATG AGCCAGCCAC AAAGTAAAGA GCAAAGATCG
    TCTGTCTGTC CTGGATTTAT GTTTCCTTCC ACTCTTTCTT CTCATCCTAT CACTCAACCT
    CCATCAGCCC CTTGGCCTGA AGCCGTGGCT ACCTTTGTCG ATTCTTTTCC TTTAAATGAC
    ACACCTCCTC CTCTACCAGC TAAGAAACAC AGGAGGCAAC AGCAGCAGGA CCAGCAGCAT
    TTTAGAAGTC TGGAAGAAAG GAAAAAACAG CACCAGGAAG GCCTCTATCT GAGTGACACT
    TTGCCTCGAA AGAAAACCAC ACCTTCCATA TCCCCACATT TCAGCAGTGC TACTATGGGG
    AGAAGCACCA CCCCAAAGGG CCACCTGCCC CTGGGACAGA GCAACAGCTG TGGCAGCGTG
    CTCCCCCACT CGCTAGCAAC CATGACCAAA GACTCAGAAT CTCGGAGGAT GCTCAGAGGA
    AAGTCCAAGA AAGGAAGAAT GAATTTCTCA GCAGGAGCAG TTAGTAATTC CAAAAGGTCA
    AGTGTCAAAG GATATAATCA CCAACAGATG AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA
    TTTTACAACC GCACAGCATC TGAATCGAAT GTCTACTTGA ACAGTTTCCA CTACCCAGAT
    CACAGCTACA AGGACCAGGC CTTTGATACT CTGAGCCTAG ACAGCTCTGA CAGCATGGAG
    ACCAGCATCT CTGCCTGCTC ACCTGACAAT ATCTCTAGTG CCAGCACTTC AAATATTGCC
    CGAATAGAGG AAATGGAGAG ACTGTTGAAG CAGGCTCATG CAGAGAAGAC AAGGCTGCTT
    GAATCCAGGG AACGAGAAAT GGAAGCCAAG AAACGCGCTC TGGAAGAAGA AAAACGACGT
    CGAGAACTCC TGGAAAAACG GTTGCAAGAA GAAACTAGCC AGAGGCAGAA GTTAATTGAA
    AAGGAAGTAA AAATAAGGGA GAAACAAAGG GCACAGGCTC GACCTCTGAC CCGCTATCTG
    CCTGTGCGGA AGGAAGACTT TGATTTGCGG AGCCACGTAG AGACTGCTGG CCACAATATC
    GATACGTGTT ATCATGTGTC AATCACAGAG AAGACCTGCA GAGGATTCCT CATCAAAATG
    GGTGGAAAAA TTAAAACCTG GAAAAAACGT TGGTTTGTTT TTGATCGGAA CAAGCGCACC
    TTCTCTTATT ATGCAGACAA GCATGAAGCT AAATTAAAAG GAGTAATATA CTTTCAAGCC
    ATTGAAGAAG TATATTATGA TCACCTCAAG AACGCTAATA AGAGTCCTAA TCCTTTACTT
    ACCTTTAGCG TCAAGACACA TGACAGAATC TACTACATGG TGGCCCCGTC GCCAGAAGCC
    ATGCGGATCT GGATGGACGT TATCGTGACT GGGGCAGAAG GCTACACTCA CTTCTTGTTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326525.1
    CDS759..4781
    Translation

    Target ORF information:

    RefSeq Version XM_015471039.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471039.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCAGAGC AGAGCCACAT GCAAAAGCAG CTGGATTTAC AGAATGGTAG CTTAGAGGCA 
    GGCTTTGCGG AGAACTCCCT CGAAAACGAT TCCCAAAACA TGATGGAGAG CCTGAGCCCA
    AAGAAATATT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTGTTCTGG CTCCTATTTA
    ACCCTCTCAC AACCTGTGCC TGCTAAAAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA
    AGTAGCCCTT CTTTGGCCAA AATTCAGGGA ACGAAGCAGT TCTCTTGTGA TGGAAGTGAG
    AAAAGTATTT CCATGAAACC GCCCACCCCT TTACGCAGCA CCTCACCCTC CCTTAGTGGA
    TATGCACTTG GGAGAGCAGA CTTTGATCAT TTTCTTGGCC GGGACGCCGA AAGGTCCTTG
    AGGCTTTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGAATAAATC CCATGACAAT
    GTCTACTTTC TCGGAGGGCT GGAAGGCCGA AAAGCATCTG GCTCGCTCCT GACCATGTGG
    AATGGAAGTT CCCTGAGTGA CACTGGCTCC TCACCCATCA ACAGGTCAGG GGCGGCCAGC
    ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATAAGCATCC AGGACAACCT GACACTTCAG
    CCCAAGTTGA GCAGACACCG GGACCCGGCA TCGGAAAACA TCAGTTTAAG AACCAGGAAG
    TACTCTAGCG GCAGCCTGAG TCACATGGGC GCCTACAGCC GCTCTCTCCC CAGGTTGCAT
    AGAGCCACCG AGAACCAGTT GGCACCCCTC AGTCTGCCTC CAAGAAGCTC TCTGGGCAAT
    TCCAAACGAA CGAAACTGGG GGAAAAGGAT CTACCTCATT GCATTATAGA CAATGACAAT
    TACCTGAATT TTTCTTCTTT GAGCTCAGGA GCTTTGCCCT ATAAAGCCTC TGCCTCCGAG
    GGCAGTGCGT ACGTGAGTTC CACCCTCAGC GTGCCTGCCA GTCCTCGAGT GGCTCGCAAG
    ATGCTTCTGG CCTCCACCTC CTCCTGCACC TCTGACGACC TTGACAGGGC TTCCTACTCG
    GGTACCAGCC CTGGTCATTC GTTTCCTCCT GGGGAGCTGG ACCGAGCATT TGCAGCCAGA
    AGGAACTTCT CGTGTGGATC TGTCGAGTTT GATGATGCAG ACTTGGACAG CCTCAGACAG
    GCCTCAGGAA CCCCCCAGCC TGTCCTGCGG GAACGGAAAA GCAGCATTAG CTCCATTTCC
    GGACGAGACG ACCTGATGGA TTATCATCGG CGGCAGAGGG AGGAGAGACT CAGGGAGCAG
    GAGATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGCCTCTG TGCTGAGTAC
    ACAAAGCCTG ACAGTCGTGT GTCCCCCGGC ACCACCGTGG CAGATGTGCA GAAAATCAAT
    AAAGAGCTGG AGAAACTGCA GCTCTCGGAT GAGGAGTCTG TGTTTGAAGA AGCCCTGGTG
    AGCCCGGACA CCAGGTACAG GTGCCACCAG AAAAGCGCTC TCCACGACGC GGACCTGGCA
    GGCTTTGGGA GCCTCAGTCA GAGCAGTGCC AGCTTCCTCC CGCCACGGGG CGCCAGGAAT
    GATGAACTTC TCGGGGACCT CGCCAGGACT CCCCCGCCGC CCTCCTCTGC CTTTCTGAAA
    GCTTCCGGCG AGTCCGCTTA CCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA
    GAACAAAGGA TTCAGGAGTT GACTGCGATG GAAGAGACCC GGATAGCAAT TCTGAACAAC
    CTTGAGGAAC TTGAACAAAA AATCAAAGAT ATAAATGACC AGATGGAGGA ATCTTCCAGA
    GAGCTGGATA TGGAATGTGC TCTCTTGGAT GGAGAACAAA AATCAGAAAC AACTGAACTT
    ATGAAAGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TCACAGAACT GGAAAAGAAC
    ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG
    AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG
    AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCCGACC TACTGGATGT TGAGAGCAAA
    CACTTTGAAG ACCTGGAATT CCAGCAGCTT GAGCGTGAAA GCCGTCTAGA TGAGGAAAAG
    GAGAACCTGA CCCAGCAACT CCTGCGTGAA GTGGCTGAGT ATCAACGGAA CATTGTTACT
    AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCTAATC ACATTGTCCA GCAGGCTCAA
    AGAGAACAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGCT GCAGAGAGAA
    AAGGAAAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT
    CCTGTCACCC CCAATACTCT GAAAGAGGGC TATATCATTG TAAATGAAAT TAATGAGTCA
    TGTGGCAGTT CCACGAATCT CCCCCCTTCC ACTCAGTTCC CTGCTGATGC TGATGCTACT
    GCCGCTGGGC CTACCACAGC TGTGCTGATG AGCCAGCCAC AAAGTAAAGA GCAAAGATCG
    TCTGTCTGTC CTGGATTTAT GTTTCCTTCC ACTCTTTCTT CTCATCCTAT CACTCAACCT
    CCATCAGCCC CTTGGCCTGA AGCCGTGGCT ACCTTTGTCG ATTCTTTTCC TTTAAATGAC
    ACACCTCCTC CTCTACCAGC TAAGAAACAC AGGAGGCAAC AGCAGCAGGA CCAGCAGCAT
    TTTAGAAGTC TGGAAGAAAG GAAAAAACAG CACCAGGAAG GCCTCTATCT GAGTGACACT
    TTGCCTCGAA AGAAAACCAC ACCTTCCATA TCCCCACATT TCAGCAGTGC TACTATGGGG
    AGAAGCACCA CCCCAAAGGG CCACCTGCCC CTGGGACAGA GCAACAGCTG TGGCAGCGTG
    CTCCCCCACT CGCTAGCAAC CATGACCAAA GACTCAGAAT CTCGGAGGAT GCTCAGAGGA
    AAGTCCAAGA AAGGAAGAAT GAATTTCTCA GCAGGAGCAG TTAGTAATTC CAAAAGGTCA
    AGTGTCAAAG GATATAATCA CCAACAGATG AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA
    TTTTACAACC GCACAGCATC TGAATCGAAT GTCTACTTGA ACAGTTTCCA CTACCCAGAT
    CACAGCTACA AGGACCAGGC CTTTGATACT CTGAGCCTAG ACAGCTCTGA CAGCATGGAG
    ACCAGCATCT CTGCCTGCTC ACCTGACAAT ATCTCTAGTG CCAGCACTTC AAATATTGCC
    CGAATAGAGG AAATGGAGAG ACTGTTGAAG CAGGCTCATG CAGAGAAGAC AAGGCTGCTT
    GAATCCAGGG AACGAGAAAT GGAAGCCAAG AAACGCGCTC TGGAAGAAGA AAAACGACGT
    CGAGAACTCC TGGAAAAACG GTTGCAAGAA GAAACTAGCC AGAGGCAGAA GTTAATTGAA
    AAGGAAGTAA AAATAAGGGA GAAACAAAGG GCACAGGCTC GACCTCTGAC CCGCTATCTG
    CCTGTGCGGA AGGAAGACTT TGATTTGCGG AGCCACGTAG AGACTGCTGG CCACAATATC
    GATACGTGTT ATCATGTGTC AATCACAGAG AAGACCTGCA GAGGATTCCT CATCAAAATG
    GGTGGAAAAA TTAAAACCTG GAAAAAACGT TGGTTTGTTT TTGATCGGAA CAAGCGCACC
    TTCTCTTATT ATGCAGACAA GCATGAAGCT AAATTAAAAG GAGTAATATA CTTTCAAGCC
    ATTGAAGAAG TATATTATGA TCACCTCAAG AACGCTAATA AGAGTCCTAA TCCTTTACTT
    ACCTTTAGCG TCAAGACACA TGACAGAATC TACTACATGG TGGCCCCGTC GCCAGAAGCC
    ATGCGGATCT GGATGGACGT TATCGTGACT GGGGCAGAAG GCTACACTCA CTTCTTGTTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87686
    Clone ID Related Accession (Same CDS sequence) XM_015471038.1 , XM_015471038.2 , XM_015471039.2 , XM_015471041.1 , XM_015471039.1
    Accession Version XM_015471041.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4023bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103810.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCAGAGC AGAGCCACAT GCAAAAGCAG CTGGATTTAC AGAATGGTAG CTTAGAGGCA 
    GGCTTTGCGG AGAACTCCCT CGAAAACGAT TCCCAAAACA TGATGGAGAG CCTGAGCCCA
    AAGAAATATT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTGTTCTGG CTCCTATTTA
    ACCCTCTCAC AACCTGTGCC TGCTAAAAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA
    AGTAGCCCTT CTTTGGCCAA AATTCAGGGA ACGAAGCAGT TCTCTTGTGA TGGAAGTGAG
    AAAAGTATTT CCATGAAACC GCCCACCCCT TTACGCAGCA CCTCACCCTC CCTTAGTGGA
    TATGCACTTG GGAGAGCAGA CTTTGATCAT TTTCTTGGCC GGGACGCCGA AAGGTCCTTG
    AGGCTTTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGAATAAATC CCATGACAAT
    GTCTACTTTC TCGGAGGGCT GGAAGGCCGA AAAGCATCTG GCTCGCTCCT GACCATGTGG
    AATGGAAGTT CCCTGAGTGA CACTGGCTCC TCACCCATCA ACAGGTCAGG GGCGGCCAGC
    ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATAAGCATCC AGGACAACCT GACACTTCAG
    CCCAAGTTGA GCAGACACCG GGACCCGGCA TCGGAAAACA TCAGTTTAAG AACCAGGAAG
    TACTCTAGCG GCAGCCTGAG TCACATGGGC GCCTACAGCC GCTCTCTCCC CAGGTTGCAT
    AGAGCCACCG AGAACCAGTT GGCACCCCTC AGTCTGCCTC CAAGAAGCTC TCTGGGCAAT
    TCCAAACGAA CGAAACTGGG GGAAAAGGAT CTACCTCATT GCATTATAGA CAATGACAAT
    TACCTGAATT TTTCTTCTTT GAGCTCAGGA GCTTTGCCCT ATAAAGCCTC TGCCTCCGAG
    GGCAGTGCGT ACGTGAGTTC CACCCTCAGC GTGCCTGCCA GTCCTCGAGT GGCTCGCAAG
    ATGCTTCTGG CCTCCACCTC CTCCTGCACC TCTGACGACC TTGACAGGGC TTCCTACTCG
    GGTACCAGCC CTGGTCATTC GTTTCCTCCT GGGGAGCTGG ACCGAGCATT TGCAGCCAGA
    AGGAACTTCT CGTGTGGATC TGTCGAGTTT GATGATGCAG ACTTGGACAG CCTCAGACAG
    GCCTCAGGAA CCCCCCAGCC TGTCCTGCGG GAACGGAAAA GCAGCATTAG CTCCATTTCC
    GGACGAGACG ACCTGATGGA TTATCATCGG CGGCAGAGGG AGGAGAGACT CAGGGAGCAG
    GAGATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGCCTCTG TGCTGAGTAC
    ACAAAGCCTG ACAGTCGTGT GTCCCCCGGC ACCACCGTGG CAGATGTGCA GAAAATCAAT
    AAAGAGCTGG AGAAACTGCA GCTCTCGGAT GAGGAGTCTG TGTTTGAAGA AGCCCTGGTG
    AGCCCGGACA CCAGGTACAG GTGCCACCAG AAAAGCGCTC TCCACGACGC GGACCTGGCA
    GGCTTTGGGA GCCTCAGTCA GAGCAGTGCC AGCTTCCTCC CGCCACGGGG CGCCAGGAAT
    GATGAACTTC TCGGGGACCT CGCCAGGACT CCCCCGCCGC CCTCCTCTGC CTTTCTGAAA
    GCTTCCGGCG AGTCCGCTTA CCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA
    GAACAAAGGA TTCAGGAGTT GACTGCGATG GAAGAGACCC GGATAGCAAT TCTGAACAAC
    CTTGAGGAAC TTGAACAAAA AATCAAAGAT ATAAATGACC AGATGGAGGA ATCTTCCAGA
    GAGCTGGATA TGGAATGTGC TCTCTTGGAT GGAGAACAAA AATCAGAAAC AACTGAACTT
    ATGAAAGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TCACAGAACT GGAAAAGAAC
    ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG
    AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG
    AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCCGACC TACTGGATGT TGAGAGCAAA
    CACTTTGAAG ACCTGGAATT CCAGCAGCTT GAGCGTGAAA GCCGTCTAGA TGAGGAAAAG
    GAGAACCTGA CCCAGCAACT CCTGCGTGAA GTGGCTGAGT ATCAACGGAA CATTGTTACT
    AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCTAATC ACATTGTCCA GCAGGCTCAA
    AGAGAACAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGCT GCAGAGAGAA
    AAGGAAAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT
    CCTGTCACCC CCAATACTCT GAAAGAGGGC TATATCATTG TAAATGAAAT TAATGAGTCA
    TGTGGCAGTT CCACGAATCT CCCCCCTTCC ACTCAGTTCC CTGCTGATGC TGATGCTACT
    GCCGCTGGGC CTACCACAGC TGTGCTGATG AGCCAGCCAC AAAGTAAAGA GCAAAGATCG
    TCTGTCTGTC CTGGATTTAT GTTTCCTTCC ACTCTTTCTT CTCATCCTAT CACTCAACCT
    CCATCAGCCC CTTGGCCTGA AGCCGTGGCT ACCTTTGTCG ATTCTTTTCC TTTAAATGAC
    ACACCTCCTC CTCTACCAGC TAAGAAACAC AGGAGGCAAC AGCAGCAGGA CCAGCAGCAT
    TTTAGAAGTC TGGAAGAAAG GAAAAAACAG CACCAGGAAG GCCTCTATCT GAGTGACACT
    TTGCCTCGAA AGAAAACCAC ACCTTCCATA TCCCCACATT TCAGCAGTGC TACTATGGGG
    AGAAGCACCA CCCCAAAGGG CCACCTGCCC CTGGGACAGA GCAACAGCTG TGGCAGCGTG
    CTCCCCCACT CGCTAGCAAC CATGACCAAA GACTCAGAAT CTCGGAGGAT GCTCAGAGGA
    AAGTCCAAGA AAGGAAGAAT GAATTTCTCA GCAGGAGCAG TTAGTAATTC CAAAAGGTCA
    AGTGTCAAAG GATATAATCA CCAACAGATG AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA
    TTTTACAACC GCACAGCATC TGAATCGAAT GTCTACTTGA ACAGTTTCCA CTACCCAGAT
    CACAGCTACA AGGACCAGGC CTTTGATACT CTGAGCCTAG ACAGCTCTGA CAGCATGGAG
    ACCAGCATCT CTGCCTGCTC ACCTGACAAT ATCTCTAGTG CCAGCACTTC AAATATTGCC
    CGAATAGAGG AAATGGAGAG ACTGTTGAAG CAGGCTCATG CAGAGAAGAC AAGGCTGCTT
    GAATCCAGGG AACGAGAAAT GGAAGCCAAG AAACGCGCTC TGGAAGAAGA AAAACGACGT
    CGAGAACTCC TGGAAAAACG GTTGCAAGAA GAAACTAGCC AGAGGCAGAA GTTAATTGAA
    AAGGAAGTAA AAATAAGGGA GAAACAAAGG GCACAGGCTC GACCTCTGAC CCGCTATCTG
    CCTGTGCGGA AGGAAGACTT TGATTTGCGG AGCCACGTAG AGACTGCTGG CCACAATATC
    GATACGTGTT ATCATGTGTC AATCACAGAG AAGACCTGCA GAGGATTCCT CATCAAAATG
    GGTGGAAAAA TTAAAACCTG GAAAAAACGT TGGTTTGTTT TTGATCGGAA CAAGCGCACC
    TTCTCTTATT ATGCAGACAA GCATGAAGCT AAATTAAAAG GAGTAATATA CTTTCAAGCC
    ATTGAAGAAG TATATTATGA TCACCTCAAG AACGCTAATA AGAGTCCTAA TCCTTTACTT
    ACCTTTAGCG TCAAGACACA TGACAGAATC TACTACATGG TGGCCCCGTC GCCAGAAGCC
    ATGCGGATCT GGATGGACGT TATCGTGACT GGGGCAGAAG GCTACACTCA CTTCTTGTTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326527.1
    CDS95..4117
    Translation

    Target ORF information:

    RefSeq Version XM_015471041.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471041.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCAGAGC AGAGCCACAT GCAAAAGCAG CTGGATTTAC AGAATGGTAG CTTAGAGGCA 
    GGCTTTGCGG AGAACTCCCT CGAAAACGAT TCCCAAAACA TGATGGAGAG CCTGAGCCCA
    AAGAAATATT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTGTTCTGG CTCCTATTTA
    ACCCTCTCAC AACCTGTGCC TGCTAAAAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA
    AGTAGCCCTT CTTTGGCCAA AATTCAGGGA ACGAAGCAGT TCTCTTGTGA TGGAAGTGAG
    AAAAGTATTT CCATGAAACC GCCCACCCCT TTACGCAGCA CCTCACCCTC CCTTAGTGGA
    TATGCACTTG GGAGAGCAGA CTTTGATCAT TTTCTTGGCC GGGACGCCGA AAGGTCCTTG
    AGGCTTTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGAATAAATC CCATGACAAT
    GTCTACTTTC TCGGAGGGCT GGAAGGCCGA AAAGCATCTG GCTCGCTCCT GACCATGTGG
    AATGGAAGTT CCCTGAGTGA CACTGGCTCC TCACCCATCA ACAGGTCAGG GGCGGCCAGC
    ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATAAGCATCC AGGACAACCT GACACTTCAG
    CCCAAGTTGA GCAGACACCG GGACCCGGCA TCGGAAAACA TCAGTTTAAG AACCAGGAAG
    TACTCTAGCG GCAGCCTGAG TCACATGGGC GCCTACAGCC GCTCTCTCCC CAGGTTGCAT
    AGAGCCACCG AGAACCAGTT GGCACCCCTC AGTCTGCCTC CAAGAAGCTC TCTGGGCAAT
    TCCAAACGAA CGAAACTGGG GGAAAAGGAT CTACCTCATT GCATTATAGA CAATGACAAT
    TACCTGAATT TTTCTTCTTT GAGCTCAGGA GCTTTGCCCT ATAAAGCCTC TGCCTCCGAG
    GGCAGTGCGT ACGTGAGTTC CACCCTCAGC GTGCCTGCCA GTCCTCGAGT GGCTCGCAAG
    ATGCTTCTGG CCTCCACCTC CTCCTGCACC TCTGACGACC TTGACAGGGC TTCCTACTCG
    GGTACCAGCC CTGGTCATTC GTTTCCTCCT GGGGAGCTGG ACCGAGCATT TGCAGCCAGA
    AGGAACTTCT CGTGTGGATC TGTCGAGTTT GATGATGCAG ACTTGGACAG CCTCAGACAG
    GCCTCAGGAA CCCCCCAGCC TGTCCTGCGG GAACGGAAAA GCAGCATTAG CTCCATTTCC
    GGACGAGACG ACCTGATGGA TTATCATCGG CGGCAGAGGG AGGAGAGACT CAGGGAGCAG
    GAGATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGCCTCTG TGCTGAGTAC
    ACAAAGCCTG ACAGTCGTGT GTCCCCCGGC ACCACCGTGG CAGATGTGCA GAAAATCAAT
    AAAGAGCTGG AGAAACTGCA GCTCTCGGAT GAGGAGTCTG TGTTTGAAGA AGCCCTGGTG
    AGCCCGGACA CCAGGTACAG GTGCCACCAG AAAAGCGCTC TCCACGACGC GGACCTGGCA
    GGCTTTGGGA GCCTCAGTCA GAGCAGTGCC AGCTTCCTCC CGCCACGGGG CGCCAGGAAT
    GATGAACTTC TCGGGGACCT CGCCAGGACT CCCCCGCCGC CCTCCTCTGC CTTTCTGAAA
    GCTTCCGGCG AGTCCGCTTA CCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA
    GAACAAAGGA TTCAGGAGTT GACTGCGATG GAAGAGACCC GGATAGCAAT TCTGAACAAC
    CTTGAGGAAC TTGAACAAAA AATCAAAGAT ATAAATGACC AGATGGAGGA ATCTTCCAGA
    GAGCTGGATA TGGAATGTGC TCTCTTGGAT GGAGAACAAA AATCAGAAAC AACTGAACTT
    ATGAAAGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TCACAGAACT GGAAAAGAAC
    ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG
    AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG
    AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCCGACC TACTGGATGT TGAGAGCAAA
    CACTTTGAAG ACCTGGAATT CCAGCAGCTT GAGCGTGAAA GCCGTCTAGA TGAGGAAAAG
    GAGAACCTGA CCCAGCAACT CCTGCGTGAA GTGGCTGAGT ATCAACGGAA CATTGTTACT
    AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCTAATC ACATTGTCCA GCAGGCTCAA
    AGAGAACAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGCT GCAGAGAGAA
    AAGGAAAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT
    CCTGTCACCC CCAATACTCT GAAAGAGGGC TATATCATTG TAAATGAAAT TAATGAGTCA
    TGTGGCAGTT CCACGAATCT CCCCCCTTCC ACTCAGTTCC CTGCTGATGC TGATGCTACT
    GCCGCTGGGC CTACCACAGC TGTGCTGATG AGCCAGCCAC AAAGTAAAGA GCAAAGATCG
    TCTGTCTGTC CTGGATTTAT GTTTCCTTCC ACTCTTTCTT CTCATCCTAT CACTCAACCT
    CCATCAGCCC CTTGGCCTGA AGCCGTGGCT ACCTTTGTCG ATTCTTTTCC TTTAAATGAC
    ACACCTCCTC CTCTACCAGC TAAGAAACAC AGGAGGCAAC AGCAGCAGGA CCAGCAGCAT
    TTTAGAAGTC TGGAAGAAAG GAAAAAACAG CACCAGGAAG GCCTCTATCT GAGTGACACT
    TTGCCTCGAA AGAAAACCAC ACCTTCCATA TCCCCACATT TCAGCAGTGC TACTATGGGG
    AGAAGCACCA CCCCAAAGGG CCACCTGCCC CTGGGACAGA GCAACAGCTG TGGCAGCGTG
    CTCCCCCACT CGCTAGCAAC CATGACCAAA GACTCAGAAT CTCGGAGGAT GCTCAGAGGA
    AAGTCCAAGA AAGGAAGAAT GAATTTCTCA GCAGGAGCAG TTAGTAATTC CAAAAGGTCA
    AGTGTCAAAG GATATAATCA CCAACAGATG AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA
    TTTTACAACC GCACAGCATC TGAATCGAAT GTCTACTTGA ACAGTTTCCA CTACCCAGAT
    CACAGCTACA AGGACCAGGC CTTTGATACT CTGAGCCTAG ACAGCTCTGA CAGCATGGAG
    ACCAGCATCT CTGCCTGCTC ACCTGACAAT ATCTCTAGTG CCAGCACTTC AAATATTGCC
    CGAATAGAGG AAATGGAGAG ACTGTTGAAG CAGGCTCATG CAGAGAAGAC AAGGCTGCTT
    GAATCCAGGG AACGAGAAAT GGAAGCCAAG AAACGCGCTC TGGAAGAAGA AAAACGACGT
    CGAGAACTCC TGGAAAAACG GTTGCAAGAA GAAACTAGCC AGAGGCAGAA GTTAATTGAA
    AAGGAAGTAA AAATAAGGGA GAAACAAAGG GCACAGGCTC GACCTCTGAC CCGCTATCTG
    CCTGTGCGGA AGGAAGACTT TGATTTGCGG AGCCACGTAG AGACTGCTGG CCACAATATC
    GATACGTGTT ATCATGTGTC AATCACAGAG AAGACCTGCA GAGGATTCCT CATCAAAATG
    GGTGGAAAAA TTAAAACCTG GAAAAAACGT TGGTTTGTTT TTGATCGGAA CAAGCGCACC
    TTCTCTTATT ATGCAGACAA GCATGAAGCT AAATTAAAAG GAGTAATATA CTTTCAAGCC
    ATTGAAGAAG TATATTATGA TCACCTCAAG AACGCTAATA AGAGTCCTAA TCCTTTACTT
    ACCTTTAGCG TCAAGACACA TGACAGAATC TACTACATGG TGGCCCCGTC GCCAGAAGCC
    ATGCGGATCT GGATGGACGT TATCGTGACT GGGGCAGAAG GCTACACTCA CTTCTTGTTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87686
    Clone ID Related Accession (Same CDS sequence) XM_015471038.1 , XM_015471038.2 , XM_015471039.2 , XM_015471041.1 , XM_015471039.1
    Accession Version XM_015471039.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4023bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103810.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCAGAGC AGAGCCACAT GCAAAAGCAG CTGGATTTAC AGAATGGTAG CTTAGAGGCA 
    GGCTTTGCGG AGAACTCCCT CGAAAACGAT TCCCAAAACA TGATGGAGAG CCTGAGCCCA
    AAGAAATATT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTGTTCTGG CTCCTATTTA
    ACCCTCTCAC AACCTGTGCC TGCTAAAAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA
    AGTAGCCCTT CTTTGGCCAA AATTCAGGGA ACGAAGCAGT TCTCTTGTGA TGGAAGTGAG
    AAAAGTATTT CCATGAAACC GCCCACCCCT TTACGCAGCA CCTCACCCTC CCTTAGTGGA
    TATGCACTTG GGAGAGCAGA CTTTGATCAT TTTCTTGGCC GGGACGCCGA AAGGTCCTTG
    AGGCTTTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGAATAAATC CCATGACAAT
    GTCTACTTTC TCGGAGGGCT GGAAGGCCGA AAAGCATCTG GCTCGCTCCT GACCATGTGG
    AATGGAAGTT CCCTGAGTGA CACTGGCTCC TCACCCATCA ACAGGTCAGG GGCGGCCAGC
    ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATAAGCATCC AGGACAACCT GACACTTCAG
    CCCAAGTTGA GCAGACACCG GGACCCGGCA TCGGAAAACA TCAGTTTAAG AACCAGGAAG
    TACTCTAGCG GCAGCCTGAG TCACATGGGC GCCTACAGCC GCTCTCTCCC CAGGTTGCAT
    AGAGCCACCG AGAACCAGTT GGCACCCCTC AGTCTGCCTC CAAGAAGCTC TCTGGGCAAT
    TCCAAACGAA CGAAACTGGG GGAAAAGGAT CTACCTCATT GCATTATAGA CAATGACAAT
    TACCTGAATT TTTCTTCTTT GAGCTCAGGA GCTTTGCCCT ATAAAGCCTC TGCCTCCGAG
    GGCAGTGCGT ACGTGAGTTC CACCCTCAGC GTGCCTGCCA GTCCTCGAGT GGCTCGCAAG
    ATGCTTCTGG CCTCCACCTC CTCCTGCACC TCTGACGACC TTGACAGGGC TTCCTACTCG
    GGTACCAGCC CTGGTCATTC GTTTCCTCCT GGGGAGCTGG ACCGAGCATT TGCAGCCAGA
    AGGAACTTCT CGTGTGGATC TGTCGAGTTT GATGATGCAG ACTTGGACAG CCTCAGACAG
    GCCTCAGGAA CCCCCCAGCC TGTCCTGCGG GAACGGAAAA GCAGCATTAG CTCCATTTCC
    GGACGAGACG ACCTGATGGA TTATCATCGG CGGCAGAGGG AGGAGAGACT CAGGGAGCAG
    GAGATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGCCTCTG TGCTGAGTAC
    ACAAAGCCTG ACAGTCGTGT GTCCCCCGGC ACCACCGTGG CAGATGTGCA GAAAATCAAT
    AAAGAGCTGG AGAAACTGCA GCTCTCGGAT GAGGAGTCTG TGTTTGAAGA AGCCCTGGTG
    AGCCCGGACA CCAGGTACAG GTGCCACCAG AAAAGCGCTC TCCACGACGC GGACCTGGCA
    GGCTTTGGGA GCCTCAGTCA GAGCAGTGCC AGCTTCCTCC CGCCACGGGG CGCCAGGAAT
    GATGAACTTC TCGGGGACCT CGCCAGGACT CCCCCGCCGC CCTCCTCTGC CTTTCTGAAA
    GCTTCCGGCG AGTCCGCTTA CCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA
    GAACAAAGGA TTCAGGAGTT GACTGCGATG GAAGAGACCC GGATAGCAAT TCTGAACAAC
    CTTGAGGAAC TTGAACAAAA AATCAAAGAT ATAAATGACC AGATGGAGGA ATCTTCCAGA
    GAGCTGGATA TGGAATGTGC TCTCTTGGAT GGAGAACAAA AATCAGAAAC AACTGAACTT
    ATGAAAGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TCACAGAACT GGAAAAGAAC
    ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG
    AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG
    AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCCGACC TACTGGATGT TGAGAGCAAA
    CACTTTGAAG ACCTGGAATT CCAGCAGCTT GAGCGTGAAA GCCGTCTAGA TGAGGAAAAG
    GAGAACCTGA CCCAGCAACT CCTGCGTGAA GTGGCTGAGT ATCAACGGAA CATTGTTACT
    AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCTAATC ACATTGTCCA GCAGGCTCAA
    AGAGAACAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGCT GCAGAGAGAA
    AAGGAAAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT
    CCTGTCACCC CCAATACTCT GAAAGAGGGC TATATCATTG TAAATGAAAT TAATGAGTCA
    TGTGGCAGTT CCACGAATCT CCCCCCTTCC ACTCAGTTCC CTGCTGATGC TGATGCTACT
    GCCGCTGGGC CTACCACAGC TGTGCTGATG AGCCAGCCAC AAAGTAAAGA GCAAAGATCG
    TCTGTCTGTC CTGGATTTAT GTTTCCTTCC ACTCTTTCTT CTCATCCTAT CACTCAACCT
    CCATCAGCCC CTTGGCCTGA AGCCGTGGCT ACCTTTGTCG ATTCTTTTCC TTTAAATGAC
    ACACCTCCTC CTCTACCAGC TAAGAAACAC AGGAGGCAAC AGCAGCAGGA CCAGCAGCAT
    TTTAGAAGTC TGGAAGAAAG GAAAAAACAG CACCAGGAAG GCCTCTATCT GAGTGACACT
    TTGCCTCGAA AGAAAACCAC ACCTTCCATA TCCCCACATT TCAGCAGTGC TACTATGGGG
    AGAAGCACCA CCCCAAAGGG CCACCTGCCC CTGGGACAGA GCAACAGCTG TGGCAGCGTG
    CTCCCCCACT CGCTAGCAAC CATGACCAAA GACTCAGAAT CTCGGAGGAT GCTCAGAGGA
    AAGTCCAAGA AAGGAAGAAT GAATTTCTCA GCAGGAGCAG TTAGTAATTC CAAAAGGTCA
    AGTGTCAAAG GATATAATCA CCAACAGATG AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA
    TTTTACAACC GCACAGCATC TGAATCGAAT GTCTACTTGA ACAGTTTCCA CTACCCAGAT
    CACAGCTACA AGGACCAGGC CTTTGATACT CTGAGCCTAG ACAGCTCTGA CAGCATGGAG
    ACCAGCATCT CTGCCTGCTC ACCTGACAAT ATCTCTAGTG CCAGCACTTC AAATATTGCC
    CGAATAGAGG AAATGGAGAG ACTGTTGAAG CAGGCTCATG CAGAGAAGAC AAGGCTGCTT
    GAATCCAGGG AACGAGAAAT GGAAGCCAAG AAACGCGCTC TGGAAGAAGA AAAACGACGT
    CGAGAACTCC TGGAAAAACG GTTGCAAGAA GAAACTAGCC AGAGGCAGAA GTTAATTGAA
    AAGGAAGTAA AAATAAGGGA GAAACAAAGG GCACAGGCTC GACCTCTGAC CCGCTATCTG
    CCTGTGCGGA AGGAAGACTT TGATTTGCGG AGCCACGTAG AGACTGCTGG CCACAATATC
    GATACGTGTT ATCATGTGTC AATCACAGAG AAGACCTGCA GAGGATTCCT CATCAAAATG
    GGTGGAAAAA TTAAAACCTG GAAAAAACGT TGGTTTGTTT TTGATCGGAA CAAGCGCACC
    TTCTCTTATT ATGCAGACAA GCATGAAGCT AAATTAAAAG GAGTAATATA CTTTCAAGCC
    ATTGAAGAAG TATATTATGA TCACCTCAAG AACGCTAATA AGAGTCCTAA TCCTTTACTT
    ACCTTTAGCG TCAAGACACA TGACAGAATC TACTACATGG TGGCCCCGTC GCCAGAAGCC
    ATGCGGATCT GGATGGACGT TATCGTGACT GGGGCAGAAG GCTACACTCA CTTCTTGTTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326525.1
    CDS368..4390
    Translation

    Target ORF information:

    RefSeq Version XM_015471039.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471039.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCAGAGC AGAGCCACAT GCAAAAGCAG CTGGATTTAC AGAATGGTAG CTTAGAGGCA 
    GGCTTTGCGG AGAACTCCCT CGAAAACGAT TCCCAAAACA TGATGGAGAG CCTGAGCCCA
    AAGAAATATT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTGTTCTGG CTCCTATTTA
    ACCCTCTCAC AACCTGTGCC TGCTAAAAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA
    AGTAGCCCTT CTTTGGCCAA AATTCAGGGA ACGAAGCAGT TCTCTTGTGA TGGAAGTGAG
    AAAAGTATTT CCATGAAACC GCCCACCCCT TTACGCAGCA CCTCACCCTC CCTTAGTGGA
    TATGCACTTG GGAGAGCAGA CTTTGATCAT TTTCTTGGCC GGGACGCCGA AAGGTCCTTG
    AGGCTTTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGAATAAATC CCATGACAAT
    GTCTACTTTC TCGGAGGGCT GGAAGGCCGA AAAGCATCTG GCTCGCTCCT GACCATGTGG
    AATGGAAGTT CCCTGAGTGA CACTGGCTCC TCACCCATCA ACAGGTCAGG GGCGGCCAGC
    ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATAAGCATCC AGGACAACCT GACACTTCAG
    CCCAAGTTGA GCAGACACCG GGACCCGGCA TCGGAAAACA TCAGTTTAAG AACCAGGAAG
    TACTCTAGCG GCAGCCTGAG TCACATGGGC GCCTACAGCC GCTCTCTCCC CAGGTTGCAT
    AGAGCCACCG AGAACCAGTT GGCACCCCTC AGTCTGCCTC CAAGAAGCTC TCTGGGCAAT
    TCCAAACGAA CGAAACTGGG GGAAAAGGAT CTACCTCATT GCATTATAGA CAATGACAAT
    TACCTGAATT TTTCTTCTTT GAGCTCAGGA GCTTTGCCCT ATAAAGCCTC TGCCTCCGAG
    GGCAGTGCGT ACGTGAGTTC CACCCTCAGC GTGCCTGCCA GTCCTCGAGT GGCTCGCAAG
    ATGCTTCTGG CCTCCACCTC CTCCTGCACC TCTGACGACC TTGACAGGGC TTCCTACTCG
    GGTACCAGCC CTGGTCATTC GTTTCCTCCT GGGGAGCTGG ACCGAGCATT TGCAGCCAGA
    AGGAACTTCT CGTGTGGATC TGTCGAGTTT GATGATGCAG ACTTGGACAG CCTCAGACAG
    GCCTCAGGAA CCCCCCAGCC TGTCCTGCGG GAACGGAAAA GCAGCATTAG CTCCATTTCC
    GGACGAGACG ACCTGATGGA TTATCATCGG CGGCAGAGGG AGGAGAGACT CAGGGAGCAG
    GAGATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGCCTCTG TGCTGAGTAC
    ACAAAGCCTG ACAGTCGTGT GTCCCCCGGC ACCACCGTGG CAGATGTGCA GAAAATCAAT
    AAAGAGCTGG AGAAACTGCA GCTCTCGGAT GAGGAGTCTG TGTTTGAAGA AGCCCTGGTG
    AGCCCGGACA CCAGGTACAG GTGCCACCAG AAAAGCGCTC TCCACGACGC GGACCTGGCA
    GGCTTTGGGA GCCTCAGTCA GAGCAGTGCC AGCTTCCTCC CGCCACGGGG CGCCAGGAAT
    GATGAACTTC TCGGGGACCT CGCCAGGACT CCCCCGCCGC CCTCCTCTGC CTTTCTGAAA
    GCTTCCGGCG AGTCCGCTTA CCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA
    GAACAAAGGA TTCAGGAGTT GACTGCGATG GAAGAGACCC GGATAGCAAT TCTGAACAAC
    CTTGAGGAAC TTGAACAAAA AATCAAAGAT ATAAATGACC AGATGGAGGA ATCTTCCAGA
    GAGCTGGATA TGGAATGTGC TCTCTTGGAT GGAGAACAAA AATCAGAAAC AACTGAACTT
    ATGAAAGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TCACAGAACT GGAAAAGAAC
    ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG
    AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG
    AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCCGACC TACTGGATGT TGAGAGCAAA
    CACTTTGAAG ACCTGGAATT CCAGCAGCTT GAGCGTGAAA GCCGTCTAGA TGAGGAAAAG
    GAGAACCTGA CCCAGCAACT CCTGCGTGAA GTGGCTGAGT ATCAACGGAA CATTGTTACT
    AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCTAATC ACATTGTCCA GCAGGCTCAA
    AGAGAACAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGCT GCAGAGAGAA
    AAGGAAAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT
    CCTGTCACCC CCAATACTCT GAAAGAGGGC TATATCATTG TAAATGAAAT TAATGAGTCA
    TGTGGCAGTT CCACGAATCT CCCCCCTTCC ACTCAGTTCC CTGCTGATGC TGATGCTACT
    GCCGCTGGGC CTACCACAGC TGTGCTGATG AGCCAGCCAC AAAGTAAAGA GCAAAGATCG
    TCTGTCTGTC CTGGATTTAT GTTTCCTTCC ACTCTTTCTT CTCATCCTAT CACTCAACCT
    CCATCAGCCC CTTGGCCTGA AGCCGTGGCT ACCTTTGTCG ATTCTTTTCC TTTAAATGAC
    ACACCTCCTC CTCTACCAGC TAAGAAACAC AGGAGGCAAC AGCAGCAGGA CCAGCAGCAT
    TTTAGAAGTC TGGAAGAAAG GAAAAAACAG CACCAGGAAG GCCTCTATCT GAGTGACACT
    TTGCCTCGAA AGAAAACCAC ACCTTCCATA TCCCCACATT TCAGCAGTGC TACTATGGGG
    AGAAGCACCA CCCCAAAGGG CCACCTGCCC CTGGGACAGA GCAACAGCTG TGGCAGCGTG
    CTCCCCCACT CGCTAGCAAC CATGACCAAA GACTCAGAAT CTCGGAGGAT GCTCAGAGGA
    AAGTCCAAGA AAGGAAGAAT GAATTTCTCA GCAGGAGCAG TTAGTAATTC CAAAAGGTCA
    AGTGTCAAAG GATATAATCA CCAACAGATG AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA
    TTTTACAACC GCACAGCATC TGAATCGAAT GTCTACTTGA ACAGTTTCCA CTACCCAGAT
    CACAGCTACA AGGACCAGGC CTTTGATACT CTGAGCCTAG ACAGCTCTGA CAGCATGGAG
    ACCAGCATCT CTGCCTGCTC ACCTGACAAT ATCTCTAGTG CCAGCACTTC AAATATTGCC
    CGAATAGAGG AAATGGAGAG ACTGTTGAAG CAGGCTCATG CAGAGAAGAC AAGGCTGCTT
    GAATCCAGGG AACGAGAAAT GGAAGCCAAG AAACGCGCTC TGGAAGAAGA AAAACGACGT
    CGAGAACTCC TGGAAAAACG GTTGCAAGAA GAAACTAGCC AGAGGCAGAA GTTAATTGAA
    AAGGAAGTAA AAATAAGGGA GAAACAAAGG GCACAGGCTC GACCTCTGAC CCGCTATCTG
    CCTGTGCGGA AGGAAGACTT TGATTTGCGG AGCCACGTAG AGACTGCTGG CCACAATATC
    GATACGTGTT ATCATGTGTC AATCACAGAG AAGACCTGCA GAGGATTCCT CATCAAAATG
    GGTGGAAAAA TTAAAACCTG GAAAAAACGT TGGTTTGTTT TTGATCGGAA CAAGCGCACC
    TTCTCTTATT ATGCAGACAA GCATGAAGCT AAATTAAAAG GAGTAATATA CTTTCAAGCC
    ATTGAAGAAG TATATTATGA TCACCTCAAG AACGCTAATA AGAGTCCTAA TCCTTTACTT
    ACCTTTAGCG TCAAGACACA TGACAGAATC TACTACATGG TGGCCCCGTC GCCAGAAGCC
    ATGCGGATCT GGATGGACGT TATCGTGACT GGGGCAGAAG GCTACACTCA CTTCTTGTTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87687
    Clone ID Related Accession (Same CDS sequence) XM_015471042.1 , XM_015471042.2
    Accession Version XM_015471042.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103810.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGATGCCGA CCTACTGGAT
    GTTGAGAGCA AACACTTTGA AGACCTGGAA TTCCAGCAGC TTGAGCGTGA AAGCCGTCTA
    GATGAGGAAA AGGAGAACCT GACCCAGCAA CTCCTGCGTG AAGTGGCTGA GTATCAACGG
    AACATTGTTA CTAGAAAGGA AAAAATTTCT GCATTGAAAA AGCAAGCTAA TCACATTGTC
    CAGCAGGCTC AAAGAGAACA AGATCATTTT GTGAAAGAAA AGAATAATTT AATAATGATG
    CTGCAGAGAG AAAAGGAAAA TCTTTGTAAT TTGGAAAAGA AATACTCCAG CCTCTCTGGG
    GGGAAAGGGT TTCCTGTCAC CCCCAATACT CTGAAAGAGG GCTATATCAT TGTAAATGAA
    ATTAATGAGT CATGTGGCAG TTCCACGAAT CTCCCCCCTT CCACTCAGTT CCCTGCTGAT
    GCTGATGCTA CTGCCGCTGG GCCTACCACA GCTGTGCTGA TGAGCCAGCC ACAAAGTAAA
    GAGCAAAGAT CGTCTGTCTG TCCTGGATTT ATGTTTCCTT CCACTCTTTC TTCTCATCCT
    ATCACTCAAC CTCCATCAGC CCCTTGGCCT GAAGCCGTGG CTACCTTTGT CGATTCTTTT
    CCTTTAAATG ACACACCTCC TCCTCTACCA GCTAAGAAAC ACAGGAGGCA ACAGCAGCAG
    GACCAGCAGC ATTTTAGAAG TCTGGAAGAA AGGAAAAAAC AGCACCAGGA AGGCCTCTAT
    CTGAGTGACA CTTTGCCTCG AAAGAAAACC ACACCTTCCA TATCCCCACA TTTCAGCAGT
    GCTACTATGG GGAGAAGCAC CACCCCAAAG GGCCACCTGC CCCTGGGACA GAGCAACAGC
    TGTGGCAGCG TGCTCCCCCA CTCGCTAGCA ACCATGACCA AAGACTCAGA ATCTCGGAGG
    ATGCTCAGAG GAAAGTCCAA GAAAGGAAGA ATGAATTTCT CAGCAGGAGC AGTTAGTAAT
    TCCAAAAGGT CAAGTGTCAA AGGATATAAT CACCAACAGA TGAGTGAAGG ACAAAGGCAG
    AAGTCTTCTG AATTTTACAA CCGCACAGCA TCTGAATCGA ATGTCTACTT GAACAGTTTC
    CACTACCCAG ATCACAGCTA CAAGGACCAG GCCTTTGATA CTCTGAGCCT AGACAGCTCT
    GACAGCATGG AGACCAGCAT CTCTGCCTGC TCACCTGACA ATATCTCTAG TGCCAGCACT
    TCAAATATTG CCCGAATAGA GGAAATGGAG AGACTGTTGA AGCAGGCTCA TGCAGAGAAG
    ACAAGGCTGC TTGAATCCAG GGAACGAGAA ATGGAAGCCA AGAAACGCGC TCTGGAAGAA
    GAAAAACGAC GTCGAGAACT CCTGGAAAAA CGGTTGCAAG AAGAAACTAG CCAGAGGCAG
    AAGTTAATTG AAAAGGAAGT AAAAATAAGG GAGAAACAAA GGGCACAGGC TCGACCTCTG
    ACCCGCTATC TGCCTGTGCG GAAGGAAGAC TTTGATTTGC GGAGCCACGT AGAGACTGCT
    GGCCACAATA TCGATACGTG TTATCATGTG TCAATCACAG AGAAGACCTG CAGAGGATTC
    CTCATCAAAA TGGGTGGAAA AATTAAAACC TGGAAAAAAC GTTGGTTTGT TTTTGATCGG
    AACAAGCGCA CCTTCTCTTA TTATGCAGAC AAGCATGAAG CTAAATTAAA AGGAGTAATA
    TACTTTCAAG CCATTGAAGA AGTATATTAT GATCACCTCA AGAACGCTAA TAAGAGTCCT
    AATCCTTTAC TTACCTTTAG CGTCAAGACA CATGACAGAA TCTACTACAT GGTGGCCCCG
    TCGCCAGAAG CCATGCGGAT CTGGATGGAC GTTATCGTGA CTGGGGCAGA AGGCTACACT
    CACTTCTTGT TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326528.1
    CDS332..4306
    Translation

    Target ORF information:

    RefSeq Version XM_015471042.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471042.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGATGCCGA CCTACTGGAT
    GTTGAGAGCA AACACTTTGA AGACCTGGAA TTCCAGCAGC TTGAGCGTGA AAGCCGTCTA
    GATGAGGAAA AGGAGAACCT GACCCAGCAA CTCCTGCGTG AAGTGGCTGA GTATCAACGG
    AACATTGTTA CTAGAAAGGA AAAAATTTCT GCATTGAAAA AGCAAGCTAA TCACATTGTC
    CAGCAGGCTC AAAGAGAACA AGATCATTTT GTGAAAGAAA AGAATAATTT AATAATGATG
    CTGCAGAGAG AAAAGGAAAA TCTTTGTAAT TTGGAAAAGA AATACTCCAG CCTCTCTGGG
    GGGAAAGGGT TTCCTGTCAC CCCCAATACT CTGAAAGAGG GCTATATCAT TGTAAATGAA
    ATTAATGAGT CATGTGGCAG TTCCACGAAT CTCCCCCCTT CCACTCAGTT CCCTGCTGAT
    GCTGATGCTA CTGCCGCTGG GCCTACCACA GCTGTGCTGA TGAGCCAGCC ACAAAGTAAA
    GAGCAAAGAT CGTCTGTCTG TCCTGGATTT ATGTTTCCTT CCACTCTTTC TTCTCATCCT
    ATCACTCAAC CTCCATCAGC CCCTTGGCCT GAAGCCGTGG CTACCTTTGT CGATTCTTTT
    CCTTTAAATG ACACACCTCC TCCTCTACCA GCTAAGAAAC ACAGGAGGCA ACAGCAGCAG
    GACCAGCAGC ATTTTAGAAG TCTGGAAGAA AGGAAAAAAC AGCACCAGGA AGGCCTCTAT
    CTGAGTGACA CTTTGCCTCG AAAGAAAACC ACACCTTCCA TATCCCCACA TTTCAGCAGT
    GCTACTATGG GGAGAAGCAC CACCCCAAAG GGCCACCTGC CCCTGGGACA GAGCAACAGC
    TGTGGCAGCG TGCTCCCCCA CTCGCTAGCA ACCATGACCA AAGACTCAGA ATCTCGGAGG
    ATGCTCAGAG GAAAGTCCAA GAAAGGAAGA ATGAATTTCT CAGCAGGAGC AGTTAGTAAT
    TCCAAAAGGT CAAGTGTCAA AGGATATAAT CACCAACAGA TGAGTGAAGG ACAAAGGCAG
    AAGTCTTCTG AATTTTACAA CCGCACAGCA TCTGAATCGA ATGTCTACTT GAACAGTTTC
    CACTACCCAG ATCACAGCTA CAAGGACCAG GCCTTTGATA CTCTGAGCCT AGACAGCTCT
    GACAGCATGG AGACCAGCAT CTCTGCCTGC TCACCTGACA ATATCTCTAG TGCCAGCACT
    TCAAATATTG CCCGAATAGA GGAAATGGAG AGACTGTTGA AGCAGGCTCA TGCAGAGAAG
    ACAAGGCTGC TTGAATCCAG GGAACGAGAA ATGGAAGCCA AGAAACGCGC TCTGGAAGAA
    GAAAAACGAC GTCGAGAACT CCTGGAAAAA CGGTTGCAAG AAGAAACTAG CCAGAGGCAG
    AAGTTAATTG AAAAGGAAGT AAAAATAAGG GAGAAACAAA GGGCACAGGC TCGACCTCTG
    ACCCGCTATC TGCCTGTGCG GAAGGAAGAC TTTGATTTGC GGAGCCACGT AGAGACTGCT
    GGCCACAATA TCGATACGTG TTATCATGTG TCAATCACAG AGAAGACCTG CAGAGGATTC
    CTCATCAAAA TGGGTGGAAA AATTAAAACC TGGAAAAAAC GTTGGTTTGT TTTTGATCGG
    AACAAGCGCA CCTTCTCTTA TTATGCAGAC AAGCATGAAG CTAAATTAAA AGGAGTAATA
    TACTTTCAAG CCATTGAAGA AGTATATTAT GATCACCTCA AGAACGCTAA TAAGAGTCCT
    AATCCTTTAC TTACCTTTAG CGTCAAGACA CATGACAGAA TCTACTACAT GGTGGCCCCG
    TCGCCAGAAG CCATGCGGAT CTGGATGGAC GTTATCGTGA CTGGGGCAGA AGGCTACACT
    CACTTCTTGT TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87687
    Clone ID Related Accession (Same CDS sequence) XM_015471042.1 , XM_015471042.2
    Accession Version XM_015471042.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471042.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGATGCCGA CCTACTGGAT
    GTTGAGAGCA AACACTTTGA AGACCTGGAA TTCCAGCAGC TTGAGCGTGA AAGCCGTCTA
    GATGAGGAAA AGGAGAACCT GACCCAGCAA CTCCTGCGTG AAGTGGCTGA GTATCAACGG
    AACATTGTTA CTAGAAAGGA AAAAATTTCT GCATTGAAAA AGCAAGCTAA TCACATTGTC
    CAGCAGGCTC AAAGAGAACA AGATCATTTT GTGAAAGAAA AGAATAATTT AATAATGATG
    CTGCAGAGAG AAAAGGAAAA TCTTTGTAAT TTGGAAAAGA AATACTCCAG CCTCTCTGGG
    GGGAAAGGGT TTCCTGTCAC CCCCAATACT CTGAAAGAGG GCTATATCAT TGTAAATGAA
    ATTAATGAGT CATGTGGCAG TTCCACGAAT CTCCCCCCTT CCACTCAGTT CCCTGCTGAT
    GCTGATGCTA CTGCCGCTGG GCCTACCACA GCTGTGCTGA TGAGCCAGCC ACAAAGTAAA
    GAGCAAAGAT CGTCTGTCTG TCCTGGATTT ATGTTTCCTT CCACTCTTTC TTCTCATCCT
    ATCACTCAAC CTCCATCAGC CCCTTGGCCT GAAGCCGTGG CTACCTTTGT CGATTCTTTT
    CCTTTAAATG ACACACCTCC TCCTCTACCA GCTAAGAAAC ACAGGAGGCA ACAGCAGCAG
    GACCAGCAGC ATTTTAGAAG TCTGGAAGAA AGGAAAAAAC AGCACCAGGA AGGCCTCTAT
    CTGAGTGACA CTTTGCCTCG AAAGAAAACC ACACCTTCCA TATCCCCACA TTTCAGCAGT
    GCTACTATGG GGAGAAGCAC CACCCCAAAG GGCCACCTGC CCCTGGGACA GAGCAACAGC
    TGTGGCAGCG TGCTCCCCCA CTCGCTAGCA ACCATGACCA AAGACTCAGA ATCTCGGAGG
    ATGCTCAGAG GAAAGTCCAA GAAAGGAAGA ATGAATTTCT CAGCAGGAGC AGTTAGTAAT
    TCCAAAAGGT CAAGTGTCAA AGGATATAAT CACCAACAGA TGAGTGAAGG ACAAAGGCAG
    AAGTCTTCTG AATTTTACAA CCGCACAGCA TCTGAATCGA ATGTCTACTT GAACAGTTTC
    CACTACCCAG ATCACAGCTA CAAGGACCAG GCCTTTGATA CTCTGAGCCT AGACAGCTCT
    GACAGCATGG AGACCAGCAT CTCTGCCTGC TCACCTGACA ATATCTCTAG TGCCAGCACT
    TCAAATATTG CCCGAATAGA GGAAATGGAG AGACTGTTGA AGCAGGCTCA TGCAGAGAAG
    ACAAGGCTGC TTGAATCCAG GGAACGAGAA ATGGAAGCCA AGAAACGCGC TCTGGAAGAA
    GAAAAACGAC GTCGAGAACT CCTGGAAAAA CGGTTGCAAG AAGAAACTAG CCAGAGGCAG
    AAGTTAATTG AAAAGGAAGT AAAAATAAGG GAGAAACAAA GGGCACAGGC TCGACCTCTG
    ACCCGCTATC TGCCTGTGCG GAAGGAAGAC TTTGATTTGC GGAGCCACGT AGAGACTGCT
    GGCCACAATA TCGATACGTG TTATCATGTG TCAATCACAG AGAAGACCTG CAGAGGATTC
    CTCATCAAAA TGGGTGGAAA AATTAAAACC TGGAAAAAAC GTTGGTTTGT TTTTGATCGG
    AACAAGCGCA CCTTCTCTTA TTATGCAGAC AAGCATGAAG CTAAATTAAA AGGAGTAATA
    TACTTTCAAG CCATTGAAGA AGTATATTAT GATCACCTCA AGAACGCTAA TAAGAGTCCT
    AATCCTTTAC TTACCTTTAG CGTCAAGACA CATGACAGAA TCTACTACAT GGTGGCCCCG
    TCGCCAGAAG CCATGCGGAT CTGGATGGAC GTTATCGTGA CTGGGGCAGA AGGCTACACT
    CACTTCTTGT TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326528.1
    CDS280..4254
    Translation

    Target ORF information:

    RefSeq Version XM_015471042.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471042.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGATGCCGA CCTACTGGAT
    GTTGAGAGCA AACACTTTGA AGACCTGGAA TTCCAGCAGC TTGAGCGTGA AAGCCGTCTA
    GATGAGGAAA AGGAGAACCT GACCCAGCAA CTCCTGCGTG AAGTGGCTGA GTATCAACGG
    AACATTGTTA CTAGAAAGGA AAAAATTTCT GCATTGAAAA AGCAAGCTAA TCACATTGTC
    CAGCAGGCTC AAAGAGAACA AGATCATTTT GTGAAAGAAA AGAATAATTT AATAATGATG
    CTGCAGAGAG AAAAGGAAAA TCTTTGTAAT TTGGAAAAGA AATACTCCAG CCTCTCTGGG
    GGGAAAGGGT TTCCTGTCAC CCCCAATACT CTGAAAGAGG GCTATATCAT TGTAAATGAA
    ATTAATGAGT CATGTGGCAG TTCCACGAAT CTCCCCCCTT CCACTCAGTT CCCTGCTGAT
    GCTGATGCTA CTGCCGCTGG GCCTACCACA GCTGTGCTGA TGAGCCAGCC ACAAAGTAAA
    GAGCAAAGAT CGTCTGTCTG TCCTGGATTT ATGTTTCCTT CCACTCTTTC TTCTCATCCT
    ATCACTCAAC CTCCATCAGC CCCTTGGCCT GAAGCCGTGG CTACCTTTGT CGATTCTTTT
    CCTTTAAATG ACACACCTCC TCCTCTACCA GCTAAGAAAC ACAGGAGGCA ACAGCAGCAG
    GACCAGCAGC ATTTTAGAAG TCTGGAAGAA AGGAAAAAAC AGCACCAGGA AGGCCTCTAT
    CTGAGTGACA CTTTGCCTCG AAAGAAAACC ACACCTTCCA TATCCCCACA TTTCAGCAGT
    GCTACTATGG GGAGAAGCAC CACCCCAAAG GGCCACCTGC CCCTGGGACA GAGCAACAGC
    TGTGGCAGCG TGCTCCCCCA CTCGCTAGCA ACCATGACCA AAGACTCAGA ATCTCGGAGG
    ATGCTCAGAG GAAAGTCCAA GAAAGGAAGA ATGAATTTCT CAGCAGGAGC AGTTAGTAAT
    TCCAAAAGGT CAAGTGTCAA AGGATATAAT CACCAACAGA TGAGTGAAGG ACAAAGGCAG
    AAGTCTTCTG AATTTTACAA CCGCACAGCA TCTGAATCGA ATGTCTACTT GAACAGTTTC
    CACTACCCAG ATCACAGCTA CAAGGACCAG GCCTTTGATA CTCTGAGCCT AGACAGCTCT
    GACAGCATGG AGACCAGCAT CTCTGCCTGC TCACCTGACA ATATCTCTAG TGCCAGCACT
    TCAAATATTG CCCGAATAGA GGAAATGGAG AGACTGTTGA AGCAGGCTCA TGCAGAGAAG
    ACAAGGCTGC TTGAATCCAG GGAACGAGAA ATGGAAGCCA AGAAACGCGC TCTGGAAGAA
    GAAAAACGAC GTCGAGAACT CCTGGAAAAA CGGTTGCAAG AAGAAACTAG CCAGAGGCAG
    AAGTTAATTG AAAAGGAAGT AAAAATAAGG GAGAAACAAA GGGCACAGGC TCGACCTCTG
    ACCCGCTATC TGCCTGTGCG GAAGGAAGAC TTTGATTTGC GGAGCCACGT AGAGACTGCT
    GGCCACAATA TCGATACGTG TTATCATGTG TCAATCACAG AGAAGACCTG CAGAGGATTC
    CTCATCAAAA TGGGTGGAAA AATTAAAACC TGGAAAAAAC GTTGGTTTGT TTTTGATCGG
    AACAAGCGCA CCTTCTCTTA TTATGCAGAC AAGCATGAAG CTAAATTAAA AGGAGTAATA
    TACTTTCAAG CCATTGAAGA AGTATATTAT GATCACCTCA AGAACGCTAA TAAGAGTCCT
    AATCCTTTAC TTACCTTTAG CGTCAAGACA CATGACAGAA TCTACTACAT GGTGGCCCCG
    TCGCCAGAAG CCATGCGGAT CTGGATGGAC GTTATCGTGA CTGGGGCAGA AGGCTACACT
    CACTTCTTGT TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87688
    Clone ID Related Accession (Same CDS sequence) XM_015471043.2 , XM_015471043.1
    Accession Version XM_015471043.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471043.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT
    GTAAATGAAA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTC
    CCTGCTGATG CTGATGCTAC TGCCGCTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA
    CAAAGTAAAG AGCATTTTAG AAGTCTGGAA GAAAGGAAAA AACAGCACCA GGAAGGCCTC
    TATCTGAGTG ACACTTTGCC TCGAAAGAAA ACCACACCTT CCATATCCCC ACATTTCAGC
    AGTGCTACTA TGGGGAGAAG CACCACCCCA AAGGGCCACC TGCCCCTGGG ACAGAGCAAC
    AGCTGTGGCA GCGTGCTCCC CCACTCGCTA GCAACCATGA CCAAAGACTC AGAATCTCGG
    AGGATGCTCA GAGGAAAGTC CAAGAAAGGA AGAATGAATT TCTCAGCAGG AGCAGTTAGT
    AATTCCAAAA GGTCAAGTGT CAAAGGATAT AATCACCAAC AGATGAGTGA AGGACAAAGG
    CAGAAGTCTT CTGAATTTTA CAACCGCACA GCATCTGAAT CGAATGTCTA CTTGAACAGT
    TTCCACTACC CAGATCACAG CTACAAGGAC CAGGCCTTTG ATACTCTGAG CCTAGACAGC
    TCTGACAGCA TGGAGACCAG CATCTCTGCC TGCTCACCTG ACAATATCTC TAGTGCCAGC
    ACTTCAAATA TTGCCCGAAT AGAGGAAATG GAGAGACTGT TGAAGCAGGC TCATGCAGAG
    AAGACAAGGC TGCTTGAATC CAGGGAACGA GAAATGGAAG CCAAGAAACG CGCTCTGGAA
    GAAGAAAAAC GACGTCGAGA ACTCCTGGAA AAACGGTTGC AAGAAGAAAC TAGCCAGAGG
    CAGAAGTTAA TTGAAAAGGA AGTAAAAATA AGGGAGAAAC AAAGGGCACA GGCTCGACCT
    CTGACCCGCT ATCTGCCTGT GCGGAAGGAA GACTTTGATT TGCGGAGCCA CGTAGAGACT
    GCTGGCCACA ATATCGATAC GTGTTATCAT GTGTCAATCA CAGAGAAGAC CTGCAGAGGA
    TTCCTCATCA AAATGGGTGG AAAAATTAAA ACCTGGAAAA AACGTTGGTT TGTTTTTGAT
    CGGAACAAGC GCACCTTCTC TTATTATGCA GACAAGCATG AAGCTAAATT AAAAGGAGTA
    ATATACTTTC AAGCCATTGA AGAAGTATAT TATGATCACC TCAAGAACGC TAATAAGAGT
    CCTAATCCTT TACTTACCTT TAGCGTCAAG ACACATGACA GAATCTACTA CATGGTGGCC
    CCGTCGCCAG AAGCCATGCG GATCTGGATG GACGTTATCG TGACTGGGGC AGAAGGCTAC
    ACTCACTTCT TGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326529.1
    CDS283..4200
    Translation

    Target ORF information:

    RefSeq Version XM_015471043.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471043.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT
    GTAAATGAAA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTC
    CCTGCTGATG CTGATGCTAC TGCCGCTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA
    CAAAGTAAAG AGCATTTTAG AAGTCTGGAA GAAAGGAAAA AACAGCACCA GGAAGGCCTC
    TATCTGAGTG ACACTTTGCC TCGAAAGAAA ACCACACCTT CCATATCCCC ACATTTCAGC
    AGTGCTACTA TGGGGAGAAG CACCACCCCA AAGGGCCACC TGCCCCTGGG ACAGAGCAAC
    AGCTGTGGCA GCGTGCTCCC CCACTCGCTA GCAACCATGA CCAAAGACTC AGAATCTCGG
    AGGATGCTCA GAGGAAAGTC CAAGAAAGGA AGAATGAATT TCTCAGCAGG AGCAGTTAGT
    AATTCCAAAA GGTCAAGTGT CAAAGGATAT AATCACCAAC AGATGAGTGA AGGACAAAGG
    CAGAAGTCTT CTGAATTTTA CAACCGCACA GCATCTGAAT CGAATGTCTA CTTGAACAGT
    TTCCACTACC CAGATCACAG CTACAAGGAC CAGGCCTTTG ATACTCTGAG CCTAGACAGC
    TCTGACAGCA TGGAGACCAG CATCTCTGCC TGCTCACCTG ACAATATCTC TAGTGCCAGC
    ACTTCAAATA TTGCCCGAAT AGAGGAAATG GAGAGACTGT TGAAGCAGGC TCATGCAGAG
    AAGACAAGGC TGCTTGAATC CAGGGAACGA GAAATGGAAG CCAAGAAACG CGCTCTGGAA
    GAAGAAAAAC GACGTCGAGA ACTCCTGGAA AAACGGTTGC AAGAAGAAAC TAGCCAGAGG
    CAGAAGTTAA TTGAAAAGGA AGTAAAAATA AGGGAGAAAC AAAGGGCACA GGCTCGACCT
    CTGACCCGCT ATCTGCCTGT GCGGAAGGAA GACTTTGATT TGCGGAGCCA CGTAGAGACT
    GCTGGCCACA ATATCGATAC GTGTTATCAT GTGTCAATCA CAGAGAAGAC CTGCAGAGGA
    TTCCTCATCA AAATGGGTGG AAAAATTAAA ACCTGGAAAA AACGTTGGTT TGTTTTTGAT
    CGGAACAAGC GCACCTTCTC TTATTATGCA GACAAGCATG AAGCTAAATT AAAAGGAGTA
    ATATACTTTC AAGCCATTGA AGAAGTATAT TATGATCACC TCAAGAACGC TAATAAGAGT
    CCTAATCCTT TACTTACCTT TAGCGTCAAG ACACATGACA GAATCTACTA CATGGTGGCC
    CCGTCGCCAG AAGCCATGCG GATCTGGATG GACGTTATCG TGACTGGGGC AGAAGGCTAC
    ACTCACTTCT TGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87688
    Clone ID Related Accession (Same CDS sequence) XM_015471043.2 , XM_015471043.1
    Accession Version XM_015471043.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103810.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT
    GTAAATGAAA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTC
    CCTGCTGATG CTGATGCTAC TGCCGCTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA
    CAAAGTAAAG AGCATTTTAG AAGTCTGGAA GAAAGGAAAA AACAGCACCA GGAAGGCCTC
    TATCTGAGTG ACACTTTGCC TCGAAAGAAA ACCACACCTT CCATATCCCC ACATTTCAGC
    AGTGCTACTA TGGGGAGAAG CACCACCCCA AAGGGCCACC TGCCCCTGGG ACAGAGCAAC
    AGCTGTGGCA GCGTGCTCCC CCACTCGCTA GCAACCATGA CCAAAGACTC AGAATCTCGG
    AGGATGCTCA GAGGAAAGTC CAAGAAAGGA AGAATGAATT TCTCAGCAGG AGCAGTTAGT
    AATTCCAAAA GGTCAAGTGT CAAAGGATAT AATCACCAAC AGATGAGTGA AGGACAAAGG
    CAGAAGTCTT CTGAATTTTA CAACCGCACA GCATCTGAAT CGAATGTCTA CTTGAACAGT
    TTCCACTACC CAGATCACAG CTACAAGGAC CAGGCCTTTG ATACTCTGAG CCTAGACAGC
    TCTGACAGCA TGGAGACCAG CATCTCTGCC TGCTCACCTG ACAATATCTC TAGTGCCAGC
    ACTTCAAATA TTGCCCGAAT AGAGGAAATG GAGAGACTGT TGAAGCAGGC TCATGCAGAG
    AAGACAAGGC TGCTTGAATC CAGGGAACGA GAAATGGAAG CCAAGAAACG CGCTCTGGAA
    GAAGAAAAAC GACGTCGAGA ACTCCTGGAA AAACGGTTGC AAGAAGAAAC TAGCCAGAGG
    CAGAAGTTAA TTGAAAAGGA AGTAAAAATA AGGGAGAAAC AAAGGGCACA GGCTCGACCT
    CTGACCCGCT ATCTGCCTGT GCGGAAGGAA GACTTTGATT TGCGGAGCCA CGTAGAGACT
    GCTGGCCACA ATATCGATAC GTGTTATCAT GTGTCAATCA CAGAGAAGAC CTGCAGAGGA
    TTCCTCATCA AAATGGGTGG AAAAATTAAA ACCTGGAAAA AACGTTGGTT TGTTTTTGAT
    CGGAACAAGC GCACCTTCTC TTATTATGCA GACAAGCATG AAGCTAAATT AAAAGGAGTA
    ATATACTTTC AAGCCATTGA AGAAGTATAT TATGATCACC TCAAGAACGC TAATAAGAGT
    CCTAATCCTT TACTTACCTT TAGCGTCAAG ACACATGACA GAATCTACTA CATGGTGGCC
    CCGTCGCCAG AAGCCATGCG GATCTGGATG GACGTTATCG TGACTGGGGC AGAAGGCTAC
    ACTCACTTCT TGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326529.1
    CDS328..4245
    Translation

    Target ORF information:

    RefSeq Version XM_015471043.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471043.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT
    GTAAATGAAA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTC
    CCTGCTGATG CTGATGCTAC TGCCGCTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA
    CAAAGTAAAG AGCATTTTAG AAGTCTGGAA GAAAGGAAAA AACAGCACCA GGAAGGCCTC
    TATCTGAGTG ACACTTTGCC TCGAAAGAAA ACCACACCTT CCATATCCCC ACATTTCAGC
    AGTGCTACTA TGGGGAGAAG CACCACCCCA AAGGGCCACC TGCCCCTGGG ACAGAGCAAC
    AGCTGTGGCA GCGTGCTCCC CCACTCGCTA GCAACCATGA CCAAAGACTC AGAATCTCGG
    AGGATGCTCA GAGGAAAGTC CAAGAAAGGA AGAATGAATT TCTCAGCAGG AGCAGTTAGT
    AATTCCAAAA GGTCAAGTGT CAAAGGATAT AATCACCAAC AGATGAGTGA AGGACAAAGG
    CAGAAGTCTT CTGAATTTTA CAACCGCACA GCATCTGAAT CGAATGTCTA CTTGAACAGT
    TTCCACTACC CAGATCACAG CTACAAGGAC CAGGCCTTTG ATACTCTGAG CCTAGACAGC
    TCTGACAGCA TGGAGACCAG CATCTCTGCC TGCTCACCTG ACAATATCTC TAGTGCCAGC
    ACTTCAAATA TTGCCCGAAT AGAGGAAATG GAGAGACTGT TGAAGCAGGC TCATGCAGAG
    AAGACAAGGC TGCTTGAATC CAGGGAACGA GAAATGGAAG CCAAGAAACG CGCTCTGGAA
    GAAGAAAAAC GACGTCGAGA ACTCCTGGAA AAACGGTTGC AAGAAGAAAC TAGCCAGAGG
    CAGAAGTTAA TTGAAAAGGA AGTAAAAATA AGGGAGAAAC AAAGGGCACA GGCTCGACCT
    CTGACCCGCT ATCTGCCTGT GCGGAAGGAA GACTTTGATT TGCGGAGCCA CGTAGAGACT
    GCTGGCCACA ATATCGATAC GTGTTATCAT GTGTCAATCA CAGAGAAGAC CTGCAGAGGA
    TTCCTCATCA AAATGGGTGG AAAAATTAAA ACCTGGAAAA AACGTTGGTT TGTTTTTGAT
    CGGAACAAGC GCACCTTCTC TTATTATGCA GACAAGCATG AAGCTAAATT AAAAGGAGTA
    ATATACTTTC AAGCCATTGA AGAAGTATAT TATGATCACC TCAAGAACGC TAATAAGAGT
    CCTAATCCTT TACTTACCTT TAGCGTCAAG ACACATGACA GAATCTACTA CATGGTGGCC
    CCGTCGCCAG AAGCCATGCG GATCTGGATG GACGTTATCG TGACTGGGGC AGAAGGCTAC
    ACTCACTTCT TGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87689
    Clone ID Related Accession (Same CDS sequence) XM_015471050.1 , XM_015471050.2
    Accession Version XM_015471050.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3774bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103810.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGCA TTTTAGAAGT
    CTGGAAGAAA GGAAAAAACA GCACCAGGAA GGCCTCTATC TGAGTGACAC TTTGCCTCGA
    AAGAAAACCA CACCTTCCAT ATCCCCACAT TTCAGCAGTG CTACTATGGG GAGAAGCACC
    ACCCCAAAGG GCCACCTGCC CCTGGGACAG AGCAACAGCT GTGGCAGCGT GCTCCCCCAC
    TCGCTAGCAA CCATGACCAA AGACTCAGAA TCTCGGAGGA TGCTCAGAGG AAAGTCCAAG
    AAAGGAAGAA TGAATTTCTC AGCAGGAGCA GTTAGTAATT CCAAAAGGTC AAGTGTCAAA
    GGATATAATC ACCAACAGAT GAGTGAAGGA CAAAGGCAGA AGTCTTCTGA ATTTTACAAC
    CGCACAGCAT CTGAATCGAA TGTCTACTTG AACAGTTTCC ACTACCCAGA TCACAGCTAC
    AAGGACCAGG CCTTTGATAC TCTGAGCCTA GACAGCTCTG ACAGCATGGA GACCAGCATC
    TCTGCCTGCT CACCTGACAA TATCTCTAGT GCCAGCACTT CAAATATTGC CCGAATAGAG
    GAAATGGAGA GACTGTTGAA GCAGGCTCAT GCAGAGAAGA CAAGGCTGCT TGAATCCAGG
    GAACGAGAAA TGGAAGCCAA GAAACGCGCT CTGGAAGAAG AAAAACGACG TCGAGAACTC
    CTGGAAAAAC GGTTGCAAGA AGAAACTAGC CAGAGGCAGA AGTTAATTGA AAAGGAAGTA
    AAAATAAGGG AGAAACAAAG GGCACAGGCT CGACCTCTGA CCCGCTATCT GCCTGTGCGG
    AAGGAAGACT TTGATTTGCG GAGCCACGTA GAGACTGCTG GCCACAATAT CGATACGTGT
    TATCATGTGT CAATCACAGA GAAGACCTGC AGAGGATTCC TCATCAAAAT GGGTGGAAAA
    ATTAAAACCT GGAAAAAACG TTGGTTTGTT TTTGATCGGA ACAAGCGCAC CTTCTCTTAT
    TATGCAGACA AGCATGAAGC TAAATTAAAA GGAGTAATAT ACTTTCAAGC CATTGAAGAA
    GTATATTATG ATCACCTCAA GAACGCTAAT AAGAGTCCTA ATCCTTTACT TACCTTTAGC
    GTCAAGACAC ATGACAGAAT CTACTACATG GTGGCCCCGT CGCCAGAAGC CATGCGGATC
    TGGATGGACG TTATCGTGAC TGGGGCAGAA GGCTACACTC ACTTCTTGTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326536.1
    CDS328..4101
    Translation

    Target ORF information:

    RefSeq Version XM_015471050.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471050.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGCA TTTTAGAAGT
    CTGGAAGAAA GGAAAAAACA GCACCAGGAA GGCCTCTATC TGAGTGACAC TTTGCCTCGA
    AAGAAAACCA CACCTTCCAT ATCCCCACAT TTCAGCAGTG CTACTATGGG GAGAAGCACC
    ACCCCAAAGG GCCACCTGCC CCTGGGACAG AGCAACAGCT GTGGCAGCGT GCTCCCCCAC
    TCGCTAGCAA CCATGACCAA AGACTCAGAA TCTCGGAGGA TGCTCAGAGG AAAGTCCAAG
    AAAGGAAGAA TGAATTTCTC AGCAGGAGCA GTTAGTAATT CCAAAAGGTC AAGTGTCAAA
    GGATATAATC ACCAACAGAT GAGTGAAGGA CAAAGGCAGA AGTCTTCTGA ATTTTACAAC
    CGCACAGCAT CTGAATCGAA TGTCTACTTG AACAGTTTCC ACTACCCAGA TCACAGCTAC
    AAGGACCAGG CCTTTGATAC TCTGAGCCTA GACAGCTCTG ACAGCATGGA GACCAGCATC
    TCTGCCTGCT CACCTGACAA TATCTCTAGT GCCAGCACTT CAAATATTGC CCGAATAGAG
    GAAATGGAGA GACTGTTGAA GCAGGCTCAT GCAGAGAAGA CAAGGCTGCT TGAATCCAGG
    GAACGAGAAA TGGAAGCCAA GAAACGCGCT CTGGAAGAAG AAAAACGACG TCGAGAACTC
    CTGGAAAAAC GGTTGCAAGA AGAAACTAGC CAGAGGCAGA AGTTAATTGA AAAGGAAGTA
    AAAATAAGGG AGAAACAAAG GGCACAGGCT CGACCTCTGA CCCGCTATCT GCCTGTGCGG
    AAGGAAGACT TTGATTTGCG GAGCCACGTA GAGACTGCTG GCCACAATAT CGATACGTGT
    TATCATGTGT CAATCACAGA GAAGACCTGC AGAGGATTCC TCATCAAAAT GGGTGGAAAA
    ATTAAAACCT GGAAAAAACG TTGGTTTGTT TTTGATCGGA ACAAGCGCAC CTTCTCTTAT
    TATGCAGACA AGCATGAAGC TAAATTAAAA GGAGTAATAT ACTTTCAAGC CATTGAAGAA
    GTATATTATG ATCACCTCAA GAACGCTAAT AAGAGTCCTA ATCCTTTACT TACCTTTAGC
    GTCAAGACAC ATGACAGAAT CTACTACATG GTGGCCCCGT CGCCAGAAGC CATGCGGATC
    TGGATGGACG TTATCGTGAC TGGGGCAGAA GGCTACACTC ACTTCTTGTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87689
    Clone ID Related Accession (Same CDS sequence) XM_015471050.1 , XM_015471050.2
    Accession Version XM_015471050.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3774bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471050.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGCA TTTTAGAAGT
    CTGGAAGAAA GGAAAAAACA GCACCAGGAA GGCCTCTATC TGAGTGACAC TTTGCCTCGA
    AAGAAAACCA CACCTTCCAT ATCCCCACAT TTCAGCAGTG CTACTATGGG GAGAAGCACC
    ACCCCAAAGG GCCACCTGCC CCTGGGACAG AGCAACAGCT GTGGCAGCGT GCTCCCCCAC
    TCGCTAGCAA CCATGACCAA AGACTCAGAA TCTCGGAGGA TGCTCAGAGG AAAGTCCAAG
    AAAGGAAGAA TGAATTTCTC AGCAGGAGCA GTTAGTAATT CCAAAAGGTC AAGTGTCAAA
    GGATATAATC ACCAACAGAT GAGTGAAGGA CAAAGGCAGA AGTCTTCTGA ATTTTACAAC
    CGCACAGCAT CTGAATCGAA TGTCTACTTG AACAGTTTCC ACTACCCAGA TCACAGCTAC
    AAGGACCAGG CCTTTGATAC TCTGAGCCTA GACAGCTCTG ACAGCATGGA GACCAGCATC
    TCTGCCTGCT CACCTGACAA TATCTCTAGT GCCAGCACTT CAAATATTGC CCGAATAGAG
    GAAATGGAGA GACTGTTGAA GCAGGCTCAT GCAGAGAAGA CAAGGCTGCT TGAATCCAGG
    GAACGAGAAA TGGAAGCCAA GAAACGCGCT CTGGAAGAAG AAAAACGACG TCGAGAACTC
    CTGGAAAAAC GGTTGCAAGA AGAAACTAGC CAGAGGCAGA AGTTAATTGA AAAGGAAGTA
    AAAATAAGGG AGAAACAAAG GGCACAGGCT CGACCTCTGA CCCGCTATCT GCCTGTGCGG
    AAGGAAGACT TTGATTTGCG GAGCCACGTA GAGACTGCTG GCCACAATAT CGATACGTGT
    TATCATGTGT CAATCACAGA GAAGACCTGC AGAGGATTCC TCATCAAAAT GGGTGGAAAA
    ATTAAAACCT GGAAAAAACG TTGGTTTGTT TTTGATCGGA ACAAGCGCAC CTTCTCTTAT
    TATGCAGACA AGCATGAAGC TAAATTAAAA GGAGTAATAT ACTTTCAAGC CATTGAAGAA
    GTATATTATG ATCACCTCAA GAACGCTAAT AAGAGTCCTA ATCCTTTACT TACCTTTAGC
    GTCAAGACAC ATGACAGAAT CTACTACATG GTGGCCCCGT CGCCAGAAGC CATGCGGATC
    TGGATGGACG TTATCGTGAC TGGGGCAGAA GGCTACACTC ACTTCTTGTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326536.1
    CDS283..4056
    Translation

    Target ORF information:

    RefSeq Version XM_015471050.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471050.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGCA TTTTAGAAGT
    CTGGAAGAAA GGAAAAAACA GCACCAGGAA GGCCTCTATC TGAGTGACAC TTTGCCTCGA
    AAGAAAACCA CACCTTCCAT ATCCCCACAT TTCAGCAGTG CTACTATGGG GAGAAGCACC
    ACCCCAAAGG GCCACCTGCC CCTGGGACAG AGCAACAGCT GTGGCAGCGT GCTCCCCCAC
    TCGCTAGCAA CCATGACCAA AGACTCAGAA TCTCGGAGGA TGCTCAGAGG AAAGTCCAAG
    AAAGGAAGAA TGAATTTCTC AGCAGGAGCA GTTAGTAATT CCAAAAGGTC AAGTGTCAAA
    GGATATAATC ACCAACAGAT GAGTGAAGGA CAAAGGCAGA AGTCTTCTGA ATTTTACAAC
    CGCACAGCAT CTGAATCGAA TGTCTACTTG AACAGTTTCC ACTACCCAGA TCACAGCTAC
    AAGGACCAGG CCTTTGATAC TCTGAGCCTA GACAGCTCTG ACAGCATGGA GACCAGCATC
    TCTGCCTGCT CACCTGACAA TATCTCTAGT GCCAGCACTT CAAATATTGC CCGAATAGAG
    GAAATGGAGA GACTGTTGAA GCAGGCTCAT GCAGAGAAGA CAAGGCTGCT TGAATCCAGG
    GAACGAGAAA TGGAAGCCAA GAAACGCGCT CTGGAAGAAG AAAAACGACG TCGAGAACTC
    CTGGAAAAAC GGTTGCAAGA AGAAACTAGC CAGAGGCAGA AGTTAATTGA AAAGGAAGTA
    AAAATAAGGG AGAAACAAAG GGCACAGGCT CGACCTCTGA CCCGCTATCT GCCTGTGCGG
    AAGGAAGACT TTGATTTGCG GAGCCACGTA GAGACTGCTG GCCACAATAT CGATACGTGT
    TATCATGTGT CAATCACAGA GAAGACCTGC AGAGGATTCC TCATCAAAAT GGGTGGAAAA
    ATTAAAACCT GGAAAAAACG TTGGTTTGTT TTTGATCGGA ACAAGCGCAC CTTCTCTTAT
    TATGCAGACA AGCATGAAGC TAAATTAAAA GGAGTAATAT ACTTTCAAGC CATTGAAGAA
    GTATATTATG ATCACCTCAA GAACGCTAAT AAGAGTCCTA ATCCTTTACT TACCTTTAGC
    GTCAAGACAC ATGACAGAAT CTACTACATG GTGGCCCCGT CGCCAGAAGC CATGCGGATC
    TGGATGGACG TTATCGTGAC TGGGGCAGAA GGCTACACTC ACTTCTTGTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87690
    Clone ID Related Accession (Same CDS sequence) XM_015471053.1 , XM_015471053.2
    Accession Version XM_015471053.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103810.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CCACCTGCCC
    CTGGGACAGA GCAACAGCTG TGGCAGCGTG CTCCCCCACT CGCTAGCAAC CATGACCAAA
    GACTCAGAAT CTCGGAGGAT GCTCAGAGGA AAGTCCAAGA AAGGAAGAAT GAATTTCTCA
    GCAGGAGCAG TTAGTAATTC CAAAAGGTCA AGTGTCAAAG GATATAATCA CCAACAGATG
    AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA TTTTACAACC GCACAGCATC TGAATCGAAT
    GTCTACTTGA ACAGTTTCCA CTACCCAGAT CACAGCTACA AGGACCAGGC CTTTGATACT
    CTGAGCCTAG ACAGCTCTGA CAGCATGGAG ACCAGCATCT CTGCCTGCTC ACCTGACAAT
    ATCTCTAGTG CCAGCACTTC AAATATTGCC CGAATAGAGG AAATGGAGAG ACTGTTGAAG
    CAGGCTCATG CAGAGAAGAC AAGGCTGCTT GAATCCAGGG AACGAGAAAT GGAAGCCAAG
    AAACGCGCTC TGGAAGAAGA AAAACGACGT CGAGAACTCC TGGAAAAACG GTTGCAAGAA
    GAAACTAGCC AGAGGCAGAA GTTAATTGAA AAGGAAGTAA AAATAAGGGA GAAACAAAGG
    GCACAGGCTC GACCTCTGAC CCGCTATCTG CCTGTGCGGA AGGAAGACTT TGATTTGCGG
    AGCCACGTAG AGACTGCTGG CCACAATATC GATACGTGTT ATCATGTGTC AATCACAGAG
    AAGACCTGCA GAGGATTCCT CATCAAAATG GGTGGAAAAA TTAAAACCTG GAAAAAACGT
    TGGTTTGTTT TTGATCGGAA CAAGCGCACC TTCTCTTATT ATGCAGACAA GCATGAAGCT
    AAATTAAAAG GAGTAATATA CTTTCAAGCC ATTGAAGAAG TATATTATGA TCACCTCAAG
    AACGCTAATA AGAGTCCTAA TCCTTTACTT ACCTTTAGCG TCAAGACACA TGACAGAATC
    TACTACATGG TGGCCCCGTC GCCAGAAGCC ATGCGGATCT GGATGGACGT TATCGTGACT
    GGGGCAGAAG GCTACACTCA CTTCTTGTTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326539.1
    CDS328..3960
    Translation

    Target ORF information:

    RefSeq Version XM_015471053.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471053.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CCACCTGCCC
    CTGGGACAGA GCAACAGCTG TGGCAGCGTG CTCCCCCACT CGCTAGCAAC CATGACCAAA
    GACTCAGAAT CTCGGAGGAT GCTCAGAGGA AAGTCCAAGA AAGGAAGAAT GAATTTCTCA
    GCAGGAGCAG TTAGTAATTC CAAAAGGTCA AGTGTCAAAG GATATAATCA CCAACAGATG
    AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA TTTTACAACC GCACAGCATC TGAATCGAAT
    GTCTACTTGA ACAGTTTCCA CTACCCAGAT CACAGCTACA AGGACCAGGC CTTTGATACT
    CTGAGCCTAG ACAGCTCTGA CAGCATGGAG ACCAGCATCT CTGCCTGCTC ACCTGACAAT
    ATCTCTAGTG CCAGCACTTC AAATATTGCC CGAATAGAGG AAATGGAGAG ACTGTTGAAG
    CAGGCTCATG CAGAGAAGAC AAGGCTGCTT GAATCCAGGG AACGAGAAAT GGAAGCCAAG
    AAACGCGCTC TGGAAGAAGA AAAACGACGT CGAGAACTCC TGGAAAAACG GTTGCAAGAA
    GAAACTAGCC AGAGGCAGAA GTTAATTGAA AAGGAAGTAA AAATAAGGGA GAAACAAAGG
    GCACAGGCTC GACCTCTGAC CCGCTATCTG CCTGTGCGGA AGGAAGACTT TGATTTGCGG
    AGCCACGTAG AGACTGCTGG CCACAATATC GATACGTGTT ATCATGTGTC AATCACAGAG
    AAGACCTGCA GAGGATTCCT CATCAAAATG GGTGGAAAAA TTAAAACCTG GAAAAAACGT
    TGGTTTGTTT TTGATCGGAA CAAGCGCACC TTCTCTTATT ATGCAGACAA GCATGAAGCT
    AAATTAAAAG GAGTAATATA CTTTCAAGCC ATTGAAGAAG TATATTATGA TCACCTCAAG
    AACGCTAATA AGAGTCCTAA TCCTTTACTT ACCTTTAGCG TCAAGACACA TGACAGAATC
    TACTACATGG TGGCCCCGTC GCCAGAAGCC ATGCGGATCT GGATGGACGT TATCGTGACT
    GGGGCAGAAG GCTACACTCA CTTCTTGTTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87690
    Clone ID Related Accession (Same CDS sequence) XM_015471053.1 , XM_015471053.2
    Accession Version XM_015471053.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471053.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CCACCTGCCC
    CTGGGACAGA GCAACAGCTG TGGCAGCGTG CTCCCCCACT CGCTAGCAAC CATGACCAAA
    GACTCAGAAT CTCGGAGGAT GCTCAGAGGA AAGTCCAAGA AAGGAAGAAT GAATTTCTCA
    GCAGGAGCAG TTAGTAATTC CAAAAGGTCA AGTGTCAAAG GATATAATCA CCAACAGATG
    AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA TTTTACAACC GCACAGCATC TGAATCGAAT
    GTCTACTTGA ACAGTTTCCA CTACCCAGAT CACAGCTACA AGGACCAGGC CTTTGATACT
    CTGAGCCTAG ACAGCTCTGA CAGCATGGAG ACCAGCATCT CTGCCTGCTC ACCTGACAAT
    ATCTCTAGTG CCAGCACTTC AAATATTGCC CGAATAGAGG AAATGGAGAG ACTGTTGAAG
    CAGGCTCATG CAGAGAAGAC AAGGCTGCTT GAATCCAGGG AACGAGAAAT GGAAGCCAAG
    AAACGCGCTC TGGAAGAAGA AAAACGACGT CGAGAACTCC TGGAAAAACG GTTGCAAGAA
    GAAACTAGCC AGAGGCAGAA GTTAATTGAA AAGGAAGTAA AAATAAGGGA GAAACAAAGG
    GCACAGGCTC GACCTCTGAC CCGCTATCTG CCTGTGCGGA AGGAAGACTT TGATTTGCGG
    AGCCACGTAG AGACTGCTGG CCACAATATC GATACGTGTT ATCATGTGTC AATCACAGAG
    AAGACCTGCA GAGGATTCCT CATCAAAATG GGTGGAAAAA TTAAAACCTG GAAAAAACGT
    TGGTTTGTTT TTGATCGGAA CAAGCGCACC TTCTCTTATT ATGCAGACAA GCATGAAGCT
    AAATTAAAAG GAGTAATATA CTTTCAAGCC ATTGAAGAAG TATATTATGA TCACCTCAAG
    AACGCTAATA AGAGTCCTAA TCCTTTACTT ACCTTTAGCG TCAAGACACA TGACAGAATC
    TACTACATGG TGGCCCCGTC GCCAGAAGCC ATGCGGATCT GGATGGACGT TATCGTGACT
    GGGGCAGAAG GCTACACTCA CTTCTTGTTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326539.1
    CDS283..3915
    Translation

    Target ORF information:

    RefSeq Version XM_015471053.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471053.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAGGAGG ATAATACCCA GAGACAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
    CCTGTGGATT CCAACAAGGA TATGGCAGAG CAGAGCCACA TGCAAAAGCA GCTGGATTTA
    CAGAATGGTA GCTTAGAGGC AGGCTTTGCG GAGAACTCCC TCGAAAACGA TTCCCAAAAC
    ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA
    GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT
    CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACGAAGCAG
    TTCTCTTGTG ATGGAAGTGA GAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC
    ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC
    CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA
    AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT
    GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCACCCATC
    AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC
    CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCGGAAAAC
    ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC
    CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT
    CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT
    TGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC
    TATAAAGCCT CTGCCTCCGA GGGCAGTGCG TACGTGAGTT CCACCCTCAG CGTGCCTGCC
    AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC
    CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG
    GACCGAGCAT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA
    GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA
    AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCATCG GCGGCAGAGG
    GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC
    CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGTG TGTCCCCCGG CACCACCGTG
    GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCGGA TGAGGAGTCT
    GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTACA GGTGCCACCA GAAAAGCGCT
    CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC
    CCGCCACGGG GCGCCAGGAA TGATGAACTT CTCGGGGACC TCGCCAGGAC TCCCCCGCCG
    CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCCGCTT ACCTAAGTAT CCTACCAAAG
    ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC
    CGGATAGCAA TTCTGAACAA CCTTGAGGAA CTTGAACAAA AAATCAAAGA TATAAATGAC
    CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA
    AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA
    ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT
    GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG
    GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC
    CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA
    AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAAC TCCTGCGTGA AGTGGCTGAG
    TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT
    CACATTGTCC AGCAGGCTCA AAGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA
    ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC
    CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CCACCTGCCC
    CTGGGACAGA GCAACAGCTG TGGCAGCGTG CTCCCCCACT CGCTAGCAAC CATGACCAAA
    GACTCAGAAT CTCGGAGGAT GCTCAGAGGA AAGTCCAAGA AAGGAAGAAT GAATTTCTCA
    GCAGGAGCAG TTAGTAATTC CAAAAGGTCA AGTGTCAAAG GATATAATCA CCAACAGATG
    AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA TTTTACAACC GCACAGCATC TGAATCGAAT
    GTCTACTTGA ACAGTTTCCA CTACCCAGAT CACAGCTACA AGGACCAGGC CTTTGATACT
    CTGAGCCTAG ACAGCTCTGA CAGCATGGAG ACCAGCATCT CTGCCTGCTC ACCTGACAAT
    ATCTCTAGTG CCAGCACTTC AAATATTGCC CGAATAGAGG AAATGGAGAG ACTGTTGAAG
    CAGGCTCATG CAGAGAAGAC AAGGCTGCTT GAATCCAGGG AACGAGAAAT GGAAGCCAAG
    AAACGCGCTC TGGAAGAAGA AAAACGACGT CGAGAACTCC TGGAAAAACG GTTGCAAGAA
    GAAACTAGCC AGAGGCAGAA GTTAATTGAA AAGGAAGTAA AAATAAGGGA GAAACAAAGG
    GCACAGGCTC GACCTCTGAC CCGCTATCTG CCTGTGCGGA AGGAAGACTT TGATTTGCGG
    AGCCACGTAG AGACTGCTGG CCACAATATC GATACGTGTT ATCATGTGTC AATCACAGAG
    AAGACCTGCA GAGGATTCCT CATCAAAATG GGTGGAAAAA TTAAAACCTG GAAAAAACGT
    TGGTTTGTTT TTGATCGGAA CAAGCGCACC TTCTCTTATT ATGCAGACAA GCATGAAGCT
    AAATTAAAAG GAGTAATATA CTTTCAAGCC ATTGAAGAAG TATATTATGA TCACCTCAAG
    AACGCTAATA AGAGTCCTAA TCCTTTACTT ACCTTTAGCG TCAAGACACA TGACAGAATC
    TACTACATGG TGGCCCCGTC GCCAGAAGCC ATGCGGATCT GGATGGACGT TATCGTGACT
    GGGGCAGAAG GCTACACTCA CTTCTTGTTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.