TOP3B cDNA ORF clone, Homo sapiens(human)

The following TOP3B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP3B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu23269 NM_001349847.2
Latest version!
Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu23269 NM_003935.5
Latest version!
Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu23269 NM_001282112.2
Latest version!
Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu23269 NM_001349845.2
Latest version!
Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu23269 NM_001282113.2
Latest version!
Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu53736 NM_001349848.2
Latest version!
Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu53736 NM_001349850.2
Latest version!
Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu23269 XM_011530482.1
Latest version!
Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu23269 XM_005261811.1
Latest version!
Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu23269 XM_024452297.1
Latest version!
Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu102339 NM_001349851.2
Latest version!
Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu102339 NM_001349852.2
Latest version!
Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu93632 XM_017029038.2
Latest version!
Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OHu93632 XM_017029038.1 Homo sapiens topoisomerase (DNA) III beta (TOP3B), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu53736 NM_001349848.1 Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu23269 NM_001282113.1 Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu102339 NM_001349851.1 Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu23269 NM_001349847.1 Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu53736 NM_001349850.1 Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu102339 NM_001349852.1 Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu23269 NM_003935.4 Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu23269 NM_001349845.1 Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu23269 NM_001282112.1 Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu23269
    Clone ID Related Accession (Same CDS sequence) NM_001282112.1 , NM_001349845.1 , NM_001282113.1 , XM_005261811.1 , NM_001349847.1 , NM_003935.4 , NM_001282113.2 , NM_001349847.2 , XM_024452297.1 , XM_011530482.1 , NM_003935.5 , NM_001349845.2 , NM_001282112.2
    Accession Version NM_001282112.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-03
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-beta-1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA025312.1, AF053082.1, AC245452.3 and BQ270362.1. On Aug 31, 2013 this sequence version replaced XM_005261810.1. Transcript Variant: This variant (2) contains an alternate 5' terminal exon compared to variant 1. Variants 1-5 encode the same protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.142918.1, SRR1660809.158208.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158188, SAMEA2158569 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGGCA TGGGCTGCAA CGAGTGTACG CACCCCTCCT GCCAGCACTC GCTGAGCATG
    CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGGGTGC TGGTGCTGGA CCCCACCTCG
    GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TAGCGCACTG CTTCGAGAAC
    GCCCACCGCG TGCGGGTGTC CGCCGACACC TGCAGTGTCT GTGAGGCCGC CTTGCTTGAT
    GTGGACTTCA ACAAGGCCAA GTCCCCACTC CCGGGCGATG AGACGCAGCA CATGGGCTGC
    GTCTTTTGTG ACCCCGTCTT CCAGGAGCTG GTGGAGCTGA AGCATGCGGC CTCCTGCCAC
    CCCATGCACC GCGGTGGACC AGGGAGAAGG CAGGGTCGAG GGCGGGGCCG GGCCAGGAGG
    CCCCCTGGGA AGCCCAACCC CAGACGGCCC AAGGACAAGA TGTCAGCCCT GGCCGCCTAC
    TTTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269041.1
    CDS213..2801
    Translation

    Target ORF information:

    RefSeq Version NM_001282112.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282112.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGGCA TGGGCTGCAA CGAGTGTACG CACCCCTCCT GCCAGCACTC GCTGAGCATG
    CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGGGTGC TGGTGCTGGA CCCCACCTCG
    GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TAGCGCACTG CTTCGAGAAC
    GCCCACCGCG TGCGGGTGTC CGCCGACACC TGCAGTGTCT GTGAGGCCGC CTTGCTTGAT
    GTGGACTTCA ACAAGGCCAA GTCCCCACTC CCGGGCGATG AGACGCAGCA CATGGGCTGC
    GTCTTTTGTG ACCCCGTCTT CCAGGAGCTG GTGGAGCTGA AGCATGCGGC CTCCTGCCAC
    CCCATGCACC GCGGTGGACC AGGGAGAAGG CAGGGTCGAG GGCGGGGCCG GGCCAGGAGG
    CCCCCTGGGA AGCCCAACCC CAGACGGCCC AAGGACAAGA TGTCAGCCCT GGCCGCCTAC
    TTTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23269
    Clone ID Related Accession (Same CDS sequence) NM_001282112.1 , NM_001349845.1 , NM_001282113.1 , XM_005261811.1 , NM_001349847.1 , NM_003935.4 , NM_001282113.2 , NM_001349847.2 , XM_024452297.1 , XM_011530482.1 , NM_003935.5 , NM_001349845.2 , NM_001282112.2
    Accession Version NM_001349845.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-03
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-beta-1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA025312.1, DA124773.1, AK290997.1, AC245452.3 and BQ270362.1. On Mar 28, 2017 this sequence version replaced XM_006724349.1. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1-5 encode the same protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.164863.1, SRR1803613.14211.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158188 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGGCA TGGGCTGCAA CGAGTGTACG CACCCCTCCT GCCAGCACTC GCTGAGCATG
    CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGGGTGC TGGTGCTGGA CCCCACCTCG
    GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TAGCGCACTG CTTCGAGAAC
    GCCCACCGCG TGCGGGTGTC CGCCGACACC TGCAGTGTCT GTGAGGCCGC CTTGCTTGAT
    GTGGACTTCA ACAAGGCCAA GTCCCCACTC CCGGGCGATG AGACGCAGCA CATGGGCTGC
    GTCTTTTGTG ACCCCGTCTT CCAGGAGCTG GTGGAGCTGA AGCATGCGGC CTCCTGCCAC
    CCCATGCACC GCGGTGGACC AGGGAGAAGG CAGGGTCGAG GGCGGGGCCG GGCCAGGAGG
    CCCCCTGGGA AGCCCAACCC CAGACGGCCC AAGGACAAGA TGTCAGCCCT GGCCGCCTAC
    TTTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336774.1
    CDS249..2837
    Translation

    Target ORF information:

    RefSeq Version NM_001349845.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349845.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGGCA TGGGCTGCAA CGAGTGTACG CACCCCTCCT GCCAGCACTC GCTGAGCATG
    CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGGGTGC TGGTGCTGGA CCCCACCTCG
    GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TAGCGCACTG CTTCGAGAAC
    GCCCACCGCG TGCGGGTGTC CGCCGACACC TGCAGTGTCT GTGAGGCCGC CTTGCTTGAT
    GTGGACTTCA ACAAGGCCAA GTCCCCACTC CCGGGCGATG AGACGCAGCA CATGGGCTGC
    GTCTTTTGTG ACCCCGTCTT CCAGGAGCTG GTGGAGCTGA AGCATGCGGC CTCCTGCCAC
    CCCATGCACC GCGGTGGACC AGGGAGAAGG CAGGGTCGAG GGCGGGGCCG GGCCAGGAGG
    CCCCCTGGGA AGCCCAACCC CAGACGGCCC AAGGACAAGA TGTCAGCCCT GGCCGCCTAC
    TTTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23269
    Clone ID Related Accession (Same CDS sequence) NM_001282112.1 , NM_001349845.1 , NM_001282113.1 , XM_005261811.1 , NM_001349847.1 , NM_003935.4 , NM_001282113.2 , NM_001349847.2 , XM_024452297.1 , XM_011530482.1 , NM_003935.5 , NM_001349845.2 , NM_001282112.2
    Accession Version NM_001282113.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-03
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-beta-1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA025312.1, AK290997.1, AC245452.3 and BQ270362.1. Transcript Variant: This variant (3) contains an alternate 5' terminal exon and uses an alternate splice site in the 5' UTR compared to variant 1. Variants 1-5 encode the same protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK290997.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2158188, SAMEA2158569 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGGCA TGGGCTGCAA CGAGTGTACG CACCCCTCCT GCCAGCACTC GCTGAGCATG
    CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGGGTGC TGGTGCTGGA CCCCACCTCG
    GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TAGCGCACTG CTTCGAGAAC
    GCCCACCGCG TGCGGGTGTC CGCCGACACC TGCAGTGTCT GTGAGGCCGC CTTGCTTGAT
    GTGGACTTCA ACAAGGCCAA GTCCCCACTC CCGGGCGATG AGACGCAGCA CATGGGCTGC
    GTCTTTTGTG ACCCCGTCTT CCAGGAGCTG GTGGAGCTGA AGCATGCGGC CTCCTGCCAC
    CCCATGCACC GCGGTGGACC AGGGAGAAGG CAGGGTCGAG GGCGGGGCCG GGCCAGGAGG
    CCCCCTGGGA AGCCCAACCC CAGACGGCCC AAGGACAAGA TGTCAGCCCT GGCCGCCTAC
    TTTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269042.1
    CDS206..2794
    Translation

    Target ORF information:

    RefSeq Version NM_001282113.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282113.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGGCA TGGGCTGCAA CGAGTGTACG CACCCCTCCT GCCAGCACTC GCTGAGCATG
    CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGGGTGC TGGTGCTGGA CCCCACCTCG
    GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TAGCGCACTG CTTCGAGAAC
    GCCCACCGCG TGCGGGTGTC CGCCGACACC TGCAGTGTCT GTGAGGCCGC CTTGCTTGAT
    GTGGACTTCA ACAAGGCCAA GTCCCCACTC CCGGGCGATG AGACGCAGCA CATGGGCTGC
    GTCTTTTGTG ACCCCGTCTT CCAGGAGCTG GTGGAGCTGA AGCATGCGGC CTCCTGCCAC
    CCCATGCACC GCGGTGGACC AGGGAGAAGG CAGGGTCGAG GGCGGGGCCG GGCCAGGAGG
    CCCCCTGGGA AGCCCAACCC CAGACGGCCC AAGGACAAGA TGTCAGCCCT GGCCGCCTAC
    TTTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23269
    Clone ID Related Accession (Same CDS sequence) NM_001282112.1 , NM_001349845.1 , NM_001282113.1 , XM_005261811.1 , NM_001349847.1 , NM_003935.4 , NM_001282113.2 , NM_001349847.2 , XM_024452297.1 , XM_011530482.1 , NM_003935.5 , NM_001349845.2 , NM_001282112.2
    Accession Version NM_001349847.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-03
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-beta-1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA025312.1, DB134726.1, AK290997.1, AC245452.3 and BQ270362.1. On Mar 28, 2017 this sequence version replaced XM_006724350.1. Transcript Variant: This variant (5) differs in the 5' UTR compared to variant 1. Variants 1-5 encode the same protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.205897.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158188 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGGCA TGGGCTGCAA CGAGTGTACG CACCCCTCCT GCCAGCACTC GCTGAGCATG
    CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGGGTGC TGGTGCTGGA CCCCACCTCG
    GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TAGCGCACTG CTTCGAGAAC
    GCCCACCGCG TGCGGGTGTC CGCCGACACC TGCAGTGTCT GTGAGGCCGC CTTGCTTGAT
    GTGGACTTCA ACAAGGCCAA GTCCCCACTC CCGGGCGATG AGACGCAGCA CATGGGCTGC
    GTCTTTTGTG ACCCCGTCTT CCAGGAGCTG GTGGAGCTGA AGCATGCGGC CTCCTGCCAC
    CCCATGCACC GCGGTGGACC AGGGAGAAGG CAGGGTCGAG GGCGGGGCCG GGCCAGGAGG
    CCCCCTGGGA AGCCCAACCC CAGACGGCCC AAGGACAAGA TGTCAGCCCT GGCCGCCTAC
    TTTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336776.1
    CDS420..3008
    Translation

    Target ORF information:

    RefSeq Version NM_001349847.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349847.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGGCA TGGGCTGCAA CGAGTGTACG CACCCCTCCT GCCAGCACTC GCTGAGCATG
    CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGGGTGC TGGTGCTGGA CCCCACCTCG
    GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TAGCGCACTG CTTCGAGAAC
    GCCCACCGCG TGCGGGTGTC CGCCGACACC TGCAGTGTCT GTGAGGCCGC CTTGCTTGAT
    GTGGACTTCA ACAAGGCCAA GTCCCCACTC CCGGGCGATG AGACGCAGCA CATGGGCTGC
    GTCTTTTGTG ACCCCGTCTT CCAGGAGCTG GTGGAGCTGA AGCATGCGGC CTCCTGCCAC
    CCCATGCACC GCGGTGGACC AGGGAGAAGG CAGGGTCGAG GGCGGGGCCG GGCCAGGAGG
    CCCCCTGGGA AGCCCAACCC CAGACGGCCC AAGGACAAGA TGTCAGCCCT GGCCGCCTAC
    TTTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23269
    Clone ID Related Accession (Same CDS sequence) NM_001282112.1 , NM_001349845.1 , NM_001282113.1 , XM_005261811.1 , NM_001349847.1 , NM_003935.4 , NM_001282113.2 , NM_001349847.2 , XM_024452297.1 , XM_011530482.1 , NM_003935.5 , NM_001349845.2 , NM_001282112.2
    Accession Version NM_003935.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-03
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-beta-1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA025312.1, BC002432.2, CR456596.1 and BQ270362.1. On Aug 18, 2013 this sequence version replaced NM_003935.3. Transcript Variant: This variant (1) represents the longest transcript. Variants 1-5 encode the same protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.174756.1, BC002432.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158188, SAMEA2162946 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGGCA TGGGCTGCAA CGAGTGTACG CACCCCTCCT GCCAGCACTC GCTGAGCATG
    CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGGGTGC TGGTGCTGGA CCCCACCTCG
    GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TAGCGCACTG CTTCGAGAAC
    GCCCACCGCG TGCGGGTGTC CGCCGACACC TGCAGTGTCT GTGAGGCCGC CTTGCTTGAT
    GTGGACTTCA ACAAGGCCAA GTCCCCACTC CCGGGCGATG AGACGCAGCA CATGGGCTGC
    GTCTTTTGTG ACCCCGTCTT CCAGGAGCTG GTGGAGCTGA AGCATGCGGC CTCCTGCCAC
    CCCATGCACC GCGGTGGACC AGGGAGAAGG CAGGGTCGAG GGCGGGGCCG GGCCAGGAGG
    CCCCCTGGGA AGCCCAACCC CAGACGGCCC AAGGACAAGA TGTCAGCCCT GGCCGCCTAC
    TTTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003926.1
    CDS529..3117
    Translation

    Target ORF information:

    RefSeq Version NM_003935.4
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_003935.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGGCA TGGGCTGCAA CGAGTGTACG CACCCCTCCT GCCAGCACTC GCTGAGCATG
    CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGGGTGC TGGTGCTGGA CCCCACCTCG
    GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TAGCGCACTG CTTCGAGAAC
    GCCCACCGCG TGCGGGTGTC CGCCGACACC TGCAGTGTCT GTGAGGCCGC CTTGCTTGAT
    GTGGACTTCA ACAAGGCCAA GTCCCCACTC CCGGGCGATG AGACGCAGCA CATGGGCTGC
    GTCTTTTGTG ACCCCGTCTT CCAGGAGCTG GTGGAGCTGA AGCATGCGGC CTCCTGCCAC
    CCCATGCACC GCGGTGGACC AGGGAGAAGG CAGGGTCGAG GGCGGGGCCG GGCCAGGAGG
    CCCCCTGGGA AGCCCAACCC CAGACGGCCC AAGGACAAGA TGTCAGCCCT GGCCGCCTAC
    TTTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23269
    Clone ID Related Accession (Same CDS sequence) NM_001282112.1 , NM_001349845.1 , NM_001282113.1 , XM_005261811.1 , NM_001349847.1 , NM_003935.4 , NM_001282113.2 , NM_001349847.2 , XM_024452297.1 , XM_011530482.1 , NM_003935.5 , NM_001349845.2 , NM_001282112.2
    Accession Version NM_001282113.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-10
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-beta-1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290997.1, AC245452.3 and BQ270362.1. On Jun 2, 2019 this sequence version replaced NM_001282113.1. Transcript Variant: This variant (3) contains an alternate 5' terminal exon and uses an alternate splice site in the 5' UTR compared to variant 1. Variants 1-5 encode the same protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK290997.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2158188, SAMEA2158569 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGGCA TGGGCTGCAA CGAGTGTACG CACCCCTCCT GCCAGCACTC GCTGAGCATG
    CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGGGTGC TGGTGCTGGA CCCCACCTCG
    GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TAGCGCACTG CTTCGAGAAC
    GCCCACCGCG TGCGGGTGTC CGCCGACACC TGCAGTGTCT GTGAGGCCGC CTTGCTTGAT
    GTGGACTTCA ACAAGGCCAA GTCCCCACTC CCGGGCGATG AGACGCAGCA CATGGGCTGC
    GTCTTTTGTG ACCCCGTCTT CCAGGAGCTG GTGGAGCTGA AGCATGCGGC CTCCTGCCAC
    CCCATGCACC GCGGTGGACC AGGGAGAAGG CAGGGTCGAG GGCGGGGCCG GGCCAGGAGG
    CCCCCTGGGA AGCCCAACCC CAGACGGCCC AAGGACAAGA TGTCAGCCCT GGCCGCCTAC
    TTTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269042.1
    CDS150..2738
    Translation

    Target ORF information:

    RefSeq Version NM_001282113.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282113.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGGCA TGGGCTGCAA CGAGTGTACG CACCCCTCCT GCCAGCACTC GCTGAGCATG
    CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGGGTGC TGGTGCTGGA CCCCACCTCG
    GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TAGCGCACTG CTTCGAGAAC
    GCCCACCGCG TGCGGGTGTC CGCCGACACC TGCAGTGTCT GTGAGGCCGC CTTGCTTGAT
    GTGGACTTCA ACAAGGCCAA GTCCCCACTC CCGGGCGATG AGACGCAGCA CATGGGCTGC
    GTCTTTTGTG ACCCCGTCTT CCAGGAGCTG GTGGAGCTGA AGCATGCGGC CTCCTGCCAC
    CCCATGCACC GCGGTGGACC AGGGAGAAGG CAGGGTCGAG GGCGGGGCCG GGCCAGGAGG
    CCCCCTGGGA AGCCCAACCC CAGACGGCCC AAGGACAAGA TGTCAGCCCT GGCCGCCTAC
    TTTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23269
    Clone ID Related Accession (Same CDS sequence) NM_001282112.1 , NM_001349845.1 , NM_001282113.1 , XM_005261811.1 , NM_001349847.1 , NM_003935.4 , NM_001282113.2 , NM_001349847.2 , XM_024452297.1 , XM_011530482.1 , NM_003935.5 , NM_001349845.2 , NM_001282112.2
    Accession Version NM_001349847.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-10
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-beta-1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA025312.1, DB134726.1, AK290997.1, AC245452.3 and BQ270362.1. On Jun 2, 2019 this sequence version replaced NM_001349847.1. Transcript Variant: This variant (5) differs in the 5' UTR compared to variant 1. Variants 1-5 encode the same protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.205897.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158188 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGGCA TGGGCTGCAA CGAGTGTACG CACCCCTCCT GCCAGCACTC GCTGAGCATG
    CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGGGTGC TGGTGCTGGA CCCCACCTCG
    GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TAGCGCACTG CTTCGAGAAC
    GCCCACCGCG TGCGGGTGTC CGCCGACACC TGCAGTGTCT GTGAGGCCGC CTTGCTTGAT
    GTGGACTTCA ACAAGGCCAA GTCCCCACTC CCGGGCGATG AGACGCAGCA CATGGGCTGC
    GTCTTTTGTG ACCCCGTCTT CCAGGAGCTG GTGGAGCTGA AGCATGCGGC CTCCTGCCAC
    CCCATGCACC GCGGTGGACC AGGGAGAAGG CAGGGTCGAG GGCGGGGCCG GGCCAGGAGG
    CCCCCTGGGA AGCCCAACCC CAGACGGCCC AAGGACAAGA TGTCAGCCCT GGCCGCCTAC
    TTTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336776.1
    CDS364..2952
    Translation

    Target ORF information:

    RefSeq Version NM_001349847.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349847.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGGCA TGGGCTGCAA CGAGTGTACG CACCCCTCCT GCCAGCACTC GCTGAGCATG
    CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGGGTGC TGGTGCTGGA CCCCACCTCG
    GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TAGCGCACTG CTTCGAGAAC
    GCCCACCGCG TGCGGGTGTC CGCCGACACC TGCAGTGTCT GTGAGGCCGC CTTGCTTGAT
    GTGGACTTCA ACAAGGCCAA GTCCCCACTC CCGGGCGATG AGACGCAGCA CATGGGCTGC
    GTCTTTTGTG ACCCCGTCTT CCAGGAGCTG GTGGAGCTGA AGCATGCGGC CTCCTGCCAC
    CCCATGCACC GCGGTGGACC AGGGAGAAGG CAGGGTCGAG GGCGGGGCCG GGCCAGGAGG
    CCCCCTGGGA AGCCCAACCC CAGACGGCCC AAGGACAAGA TGTCAGCCCT GGCCGCCTAC
    TTTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23269
    Clone ID Related Accession (Same CDS sequence) NM_001282112.1 , NM_001349845.1 , NM_001282113.1 , XM_005261811.1 , NM_001349847.1 , NM_003935.4 , NM_001282113.2 , NM_001349847.2 , XM_024452297.1 , XM_011530482.1 , NM_003935.5 , NM_001349845.2 , NM_001282112.2
    Accession Version NM_003935.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-10
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-beta-1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA025312.1, BC002432.2, CR456596.1 and BQ270362.1. On Jun 2, 2019 this sequence version replaced NM_003935.4. Transcript Variant: This variant (1) represents the longest transcript. Variants 1-5 encode the same protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.174756.1, BC002432.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158188, SAMEA2162946 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGGCA TGGGCTGCAA CGAGTGTACG CACCCCTCCT GCCAGCACTC GCTGAGCATG
    CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGGGTGC TGGTGCTGGA CCCCACCTCG
    GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TAGCGCACTG CTTCGAGAAC
    GCCCACCGCG TGCGGGTGTC CGCCGACACC TGCAGTGTCT GTGAGGCCGC CTTGCTTGAT
    GTGGACTTCA ACAAGGCCAA GTCCCCACTC CCGGGCGATG AGACGCAGCA CATGGGCTGC
    GTCTTTTGTG ACCCCGTCTT CCAGGAGCTG GTGGAGCTGA AGCATGCGGC CTCCTGCCAC
    CCCATGCACC GCGGTGGACC AGGGAGAAGG CAGGGTCGAG GGCGGGGCCG GGCCAGGAGG
    CCCCCTGGGA AGCCCAACCC CAGACGGCCC AAGGACAAGA TGTCAGCCCT GGCCGCCTAC
    TTTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003926.1
    CDS473..3061
    Translation

    Target ORF information:

    RefSeq Version NM_003935.5
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_003935.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGGCA TGGGCTGCAA CGAGTGTACG CACCCCTCCT GCCAGCACTC GCTGAGCATG
    CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGGGTGC TGGTGCTGGA CCCCACCTCG
    GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TAGCGCACTG CTTCGAGAAC
    GCCCACCGCG TGCGGGTGTC CGCCGACACC TGCAGTGTCT GTGAGGCCGC CTTGCTTGAT
    GTGGACTTCA ACAAGGCCAA GTCCCCACTC CCGGGCGATG AGACGCAGCA CATGGGCTGC
    GTCTTTTGTG ACCCCGTCTT CCAGGAGCTG GTGGAGCTGA AGCATGCGGC CTCCTGCCAC
    CCCATGCACC GCGGTGGACC AGGGAGAAGG CAGGGTCGAG GGCGGGGCCG GGCCAGGAGG
    CCCCCTGGGA AGCCCAACCC CAGACGGCCC AAGGACAAGA TGTCAGCCCT GGCCGCCTAC
    TTTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23269
    Clone ID Related Accession (Same CDS sequence) NM_001282112.1 , NM_001349845.1 , NM_001282113.1 , XM_005261811.1 , NM_001349847.1 , NM_003935.4 , NM_001282113.2 , NM_001349847.2 , XM_024452297.1 , XM_011530482.1 , NM_003935.5 , NM_001349845.2 , NM_001282112.2
    Accession Version NM_001349845.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-10
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-beta-1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA025312.1, DA124773.1, AK290997.1, AC245452.3 and BQ270362.1. On Jun 2, 2019 this sequence version replaced NM_001349845.1. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1-5 encode the same protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.164863.1, SRR1803613.14211.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158188 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGGCA TGGGCTGCAA CGAGTGTACG CACCCCTCCT GCCAGCACTC GCTGAGCATG
    CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGGGTGC TGGTGCTGGA CCCCACCTCG
    GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TAGCGCACTG CTTCGAGAAC
    GCCCACCGCG TGCGGGTGTC CGCCGACACC TGCAGTGTCT GTGAGGCCGC CTTGCTTGAT
    GTGGACTTCA ACAAGGCCAA GTCCCCACTC CCGGGCGATG AGACGCAGCA CATGGGCTGC
    GTCTTTTGTG ACCCCGTCTT CCAGGAGCTG GTGGAGCTGA AGCATGCGGC CTCCTGCCAC
    CCCATGCACC GCGGTGGACC AGGGAGAAGG CAGGGTCGAG GGCGGGGCCG GGCCAGGAGG
    CCCCCTGGGA AGCCCAACCC CAGACGGCCC AAGGACAAGA TGTCAGCCCT GGCCGCCTAC
    TTTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336774.1
    CDS193..2781
    Translation

    Target ORF information:

    RefSeq Version NM_001349845.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349845.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGGCA TGGGCTGCAA CGAGTGTACG CACCCCTCCT GCCAGCACTC GCTGAGCATG
    CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGGGTGC TGGTGCTGGA CCCCACCTCG
    GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TAGCGCACTG CTTCGAGAAC
    GCCCACCGCG TGCGGGTGTC CGCCGACACC TGCAGTGTCT GTGAGGCCGC CTTGCTTGAT
    GTGGACTTCA ACAAGGCCAA GTCCCCACTC CCGGGCGATG AGACGCAGCA CATGGGCTGC
    GTCTTTTGTG ACCCCGTCTT CCAGGAGCTG GTGGAGCTGA AGCATGCGGC CTCCTGCCAC
    CCCATGCACC GCGGTGGACC AGGGAGAAGG CAGGGTCGAG GGCGGGGCCG GGCCAGGAGG
    CCCCCTGGGA AGCCCAACCC CAGACGGCCC AAGGACAAGA TGTCAGCCCT GGCCGCCTAC
    TTTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23269
    Clone ID Related Accession (Same CDS sequence) NM_001282112.1 , NM_001349845.1 , NM_001282113.1 , XM_005261811.1 , NM_001349847.1 , NM_003935.4 , NM_001282113.2 , NM_001349847.2 , XM_024452297.1 , XM_011530482.1 , NM_003935.5 , NM_001349845.2 , NM_001282112.2
    Accession Version NM_001282112.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-28
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-beta-1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA025312.1, AF053082.1, AC245452.3 and BQ270362.1. On Jun 2, 2019 this sequence version replaced NM_001282112.1. Transcript Variant: This variant (2) contains an alternate 5' terminal exon compared to variant 1. Variants 1-5 encode the same protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.7267.1, SRR1660809.158208.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158188, SAMEA2158569 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGGCA TGGGCTGCAA CGAGTGTACG CACCCCTCCT GCCAGCACTC GCTGAGCATG
    CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGGGTGC TGGTGCTGGA CCCCACCTCG
    GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TAGCGCACTG CTTCGAGAAC
    GCCCACCGCG TGCGGGTGTC CGCCGACACC TGCAGTGTCT GTGAGGCCGC CTTGCTTGAT
    GTGGACTTCA ACAAGGCCAA GTCCCCACTC CCGGGCGATG AGACGCAGCA CATGGGCTGC
    GTCTTTTGTG ACCCCGTCTT CCAGGAGCTG GTGGAGCTGA AGCATGCGGC CTCCTGCCAC
    CCCATGCACC GCGGTGGACC AGGGAGAAGG CAGGGTCGAG GGCGGGGCCG GGCCAGGAGG
    CCCCCTGGGA AGCCCAACCC CAGACGGCCC AAGGACAAGA TGTCAGCCCT GGCCGCCTAC
    TTTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269041.1
    CDS157..2745
    Translation

    Target ORF information:

    RefSeq Version NM_001282112.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282112.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGGCA TGGGCTGCAA CGAGTGTACG CACCCCTCCT GCCAGCACTC GCTGAGCATG
    CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGGGTGC TGGTGCTGGA CCCCACCTCG
    GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TAGCGCACTG CTTCGAGAAC
    GCCCACCGCG TGCGGGTGTC CGCCGACACC TGCAGTGTCT GTGAGGCCGC CTTGCTTGAT
    GTGGACTTCA ACAAGGCCAA GTCCCCACTC CCGGGCGATG AGACGCAGCA CATGGGCTGC
    GTCTTTTGTG ACCCCGTCTT CCAGGAGCTG GTGGAGCTGA AGCATGCGGC CTCCTGCCAC
    CCCATGCACC GCGGTGGACC AGGGAGAAGG CAGGGTCGAG GGCGGGGCCG GGCCAGGAGG
    CCCCCTGGGA AGCCCAACCC CAGACGGCCC AAGGACAAGA TGTCAGCCCT GGCCGCCTAC
    TTTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53736
    Clone ID Related Accession (Same CDS sequence) NM_001349850.2 , NM_001349848.1 , NM_001349848.2 , NM_001349850.1
    Accession Version NM_001349850.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2181bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-10
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-beta-1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA025312.1, DA124773.1, DB146985.1, DB150453.1, AK290997.1, AC245452.3 and BQ270362.1. On Jun 2, 2019 this sequence version replaced NM_001349850.1. Transcript Variant: This variant (7) contains an alternate exon structure in the 5' region and contains an alternate exon in its 5' UTR, resulting in the use of a downstream start codon compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. Variants 6 and 7 encode the same protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.246203.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAACAAGG CCCATGGTGG CGAGAAGACC GTGTTCCGGG CCAGGTTTAG CTCCATCACG 
    GACACAGACA TCTGTAATGC CATGGCCTGC CTAGGCGAGC CTGACCACAA CGAGGCGCTC
    TCAGTGGATG CTCGCCAGGA GCTGGACCTG CGAATCGGCT GTGCATTCAC CAGGTTTCAG
    ACTAAATATT TCCAGGGGAA ATACGGTGAT TTAGACAGCT CTCTCATCTC CTTTGGGCCG
    TGTCAGACTC CAACCCTGGG ATTCTGTGTG GAGAGACATG ATAAAATCCA GTCCTTCAAA
    CCAGAGACCT ACTGGGTGCT GCAGGCCAAG GTTAACACTG ACAAAGACAG ATCTCTCCTT
    TTGGACTGGG ACCGAGTAAG AGTGTTTGAC CGGGAGATCG CACAGATGTT TTTAAACATG
    ACAAAGCTGG AGAAGGAAGC CCAGGTGGAG GCCACAAGCA GGAAAGAAAA GGCCAAGCAG
    AGGCCCCTGG CCCTGAACAC TGTGGAGATG CTGCGTGTGG CCAGCTCTTC TCTGGGCATG
    GGGCCGCAGC ACGCCATGCA GACGGCTGAG CGGCTCTACA CGCAAGGCTA CATCAGCTAC
    CCACGGACAG AGACCACCCA CTACCCTGAG AACTTTGACC TGAAGGGCTC TCTGCGGCAG
    CAGGCCAACC ACCCCTACTG GGCCGACACG GTGAAGCGGT TGTTAGCAGA AGGTATCAAC
    CGCCCGCGGA AAGGCCATGA CGCCGGCGAC CATCCCCCCA TCACCCCCAT GAAGTCTGCC
    ACAGAGGCCG AATTAGGGGG TGACGCGTGG CGGCTCTATG AGTACATCAC CAGACACTTC
    ATCGCCACGG TCAGCCATGA CTGCAAGTAC CTGCAGAGCA CCATCTCCTT CAGAATTGGG
    CCCGAGCTCT TCACCTGCTC CGGGAAGACC GTCCTCTCAC CAGGCTTCAC GGAGGTCATG
    CCCTGGCAGA GCGTGCCCCT GGAGGAGAGC CTGCCCACTT GCCAGCGGGG TGATGCCTTC
    CCTGTGGGCG AGGTGAAGAT GCTGGAGAAG CAGACGAACC CACCCGACTA CCTGACGGAG
    GCCGAGCTCA TCACGCTCAT GGAGAAGCAT GGCATCGGCA CGGATGCCAG CATCCCTGTG
    CATATCAACA ACATCTGCCA GCGCAACTAT GTCACGGTGG AGAGCGGGCG CCGGCTCAAG
    CCCACCAACC TCGGCATCGT CCTGGTGCAC GGCTACTATA AGATTGATGC AGAGCTGGTG
    CTCCCCACCA TCCGCAGTGC AGTGGAGAAG CAGCTGAACC TGATCGCCCA GGGCAAGGCC
    GACTACCGCC AGGTCCTGGG CCACACCCTG GACGTGTTCA AGAGGAAGTT CCACTACTTT
    GTCGACTCCA TTGCTGGCAT GGATGAGTTG ATGGAGGTGT CTTTCTCGCC CCTGGCGGCC
    ACAGGCAAGC CCCTCTCACG CTGTGGGAAG TGCCACCGCT TCATGAAGTA CATCCAGGCC
    AAGCCAAGCC GCCTGCACTG CTCCCACTGC GATGAGACCT ACACGCTCCC CCAGAACGGC
    ACCATCAAGC TCTACAAGGA GCTCCGCTGC CCTCTGGATG ACTTCGAGCT GGTCCTGTGG
    TCATCAGGCT CTCGGGGCAA GAGCTACCCG CTGTGCCCCT ACTGCTACAA CCACCCACCC
    TTCCGAGACA TGAAGAAAGG CATGGGCTGC AACGAGTGTA CGCACCCCTC CTGCCAGCAC
    TCGCTGAGCA TGCTGGGCAT CGGCCAGTGC GTGGAATGTG AGAGCGGGGT GCTGGTGCTG
    GACCCCACCT CGGGCCCCAA GTGGAAGGTG GCCTGCAACA AGTGCAACGT GGTAGCGCAC
    TGCTTCGAGA ACGCCCACCG CGTGCGGGTG TCCGCCGACA CCTGCAGTGT CTGTGAGGCC
    GCCTTGCTTG ATGTGGACTT CAACAAGGCC AAGTCCCCAC TCCCGGGCGA TGAGACGCAG
    CACATGGGCT GCGTCTTTTG TGACCCCGTC TTCCAGGAGC TGGTGGAGCT GAAGCATGCG
    GCCTCCTGCC ACCCCATGCA CCGCGGTGGA CCAGGGAGAA GGCAGGGTCG AGGGCGGGGC
    CGGGCCAGGA GGCCCCCTGG GAAGCCCAAC CCCAGACGGC CCAAGGACAA GATGTCAGCC
    CTGGCCGCCT ACTTTGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336779.1
    CDS683..2863
    Translation

    Target ORF information:

    RefSeq Version NM_001349850.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349850.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAACAAGG CCCATGGTGG CGAGAAGACC GTGTTCCGGG CCAGGTTTAG CTCCATCACG 
    GACACAGACA TCTGTAATGC CATGGCCTGC CTAGGCGAGC CTGACCACAA CGAGGCGCTC
    TCAGTGGATG CTCGCCAGGA GCTGGACCTG CGAATCGGCT GTGCATTCAC CAGGTTTCAG
    ACTAAATATT TCCAGGGGAA ATACGGTGAT TTAGACAGCT CTCTCATCTC CTTTGGGCCG
    TGTCAGACTC CAACCCTGGG ATTCTGTGTG GAGAGACATG ATAAAATCCA GTCCTTCAAA
    CCAGAGACCT ACTGGGTGCT GCAGGCCAAG GTTAACACTG ACAAAGACAG ATCTCTCCTT
    TTGGACTGGG ACCGAGTAAG AGTGTTTGAC CGGGAGATCG CACAGATGTT TTTAAACATG
    ACAAAGCTGG AGAAGGAAGC CCAGGTGGAG GCCACAAGCA GGAAAGAAAA GGCCAAGCAG
    AGGCCCCTGG CCCTGAACAC TGTGGAGATG CTGCGTGTGG CCAGCTCTTC TCTGGGCATG
    GGGCCGCAGC ACGCCATGCA GACGGCTGAG CGGCTCTACA CGCAAGGCTA CATCAGCTAC
    CCACGGACAG AGACCACCCA CTACCCTGAG AACTTTGACC TGAAGGGCTC TCTGCGGCAG
    CAGGCCAACC ACCCCTACTG GGCCGACACG GTGAAGCGGT TGTTAGCAGA AGGTATCAAC
    CGCCCGCGGA AAGGCCATGA CGCCGGCGAC CATCCCCCCA TCACCCCCAT GAAGTCTGCC
    ACAGAGGCCG AATTAGGGGG TGACGCGTGG CGGCTCTATG AGTACATCAC CAGACACTTC
    ATCGCCACGG TCAGCCATGA CTGCAAGTAC CTGCAGAGCA CCATCTCCTT CAGAATTGGG
    CCCGAGCTCT TCACCTGCTC CGGGAAGACC GTCCTCTCAC CAGGCTTCAC GGAGGTCATG
    CCCTGGCAGA GCGTGCCCCT GGAGGAGAGC CTGCCCACTT GCCAGCGGGG TGATGCCTTC
    CCTGTGGGCG AGGTGAAGAT GCTGGAGAAG CAGACGAACC CACCCGACTA CCTGACGGAG
    GCCGAGCTCA TCACGCTCAT GGAGAAGCAT GGCATCGGCA CGGATGCCAG CATCCCTGTG
    CATATCAACA ACATCTGCCA GCGCAACTAT GTCACGGTGG AGAGCGGGCG CCGGCTCAAG
    CCCACCAACC TCGGCATCGT CCTGGTGCAC GGCTACTATA AGATTGATGC AGAGCTGGTG
    CTCCCCACCA TCCGCAGTGC AGTGGAGAAG CAGCTGAACC TGATCGCCCA GGGCAAGGCC
    GACTACCGCC AGGTCCTGGG CCACACCCTG GACGTGTTCA AGAGGAAGTT CCACTACTTT
    GTCGACTCCA TTGCTGGCAT GGATGAGTTG ATGGAGGTGT CTTTCTCGCC CCTGGCGGCC
    ACAGGCAAGC CCCTCTCACG CTGTGGGAAG TGCCACCGCT TCATGAAGTA CATCCAGGCC
    AAGCCAAGCC GCCTGCACTG CTCCCACTGC GATGAGACCT ACACGCTCCC CCAGAACGGC
    ACCATCAAGC TCTACAAGGA GCTCCGCTGC CCTCTGGATG ACTTCGAGCT GGTCCTGTGG
    TCATCAGGCT CTCGGGGCAA GAGCTACCCG CTGTGCCCCT ACTGCTACAA CCACCCACCC
    TTCCGAGACA TGAAGAAAGG CATGGGCTGC AACGAGTGTA CGCACCCCTC CTGCCAGCAC
    TCGCTGAGCA TGCTGGGCAT CGGCCAGTGC GTGGAATGTG AGAGCGGGGT GCTGGTGCTG
    GACCCCACCT CGGGCCCCAA GTGGAAGGTG GCCTGCAACA AGTGCAACGT GGTAGCGCAC
    TGCTTCGAGA ACGCCCACCG CGTGCGGGTG TCCGCCGACA CCTGCAGTGT CTGTGAGGCC
    GCCTTGCTTG ATGTGGACTT CAACAAGGCC AAGTCCCCAC TCCCGGGCGA TGAGACGCAG
    CACATGGGCT GCGTCTTTTG TGACCCCGTC TTCCAGGAGC TGGTGGAGCT GAAGCATGCG
    GCCTCCTGCC ACCCCATGCA CCGCGGTGGA CCAGGGAGAA GGCAGGGTCG AGGGCGGGGC
    CGGGCCAGGA GGCCCCCTGG GAAGCCCAAC CCCAGACGGC CCAAGGACAA GATGTCAGCC
    CTGGCCGCCT ACTTTGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53736
    Clone ID Related Accession (Same CDS sequence) NM_001349850.2 , NM_001349848.1 , NM_001349848.2 , NM_001349850.1
    Accession Version NM_001349848.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2181bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-03
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-beta-1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA025312.1, DB165571.1, DR003213.1, AK290997.1, AC245452.3 and BQ270362.1. On Mar 28, 2017 this sequence version replaced XM_005261813.2. Transcript Variant: This variant (6) uses an alternate splice site in its 5' terminal exon and contains an alternate exon in its 5' UTR, resulting in the use of a downstream start codon compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. Variants 6 and 7 encode the same protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.152412.1, SRR1660809.53960.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158569 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAACAAGG CCCATGGTGG CGAGAAGACC GTGTTCCGGG CCAGGTTTAG CTCCATCACG 
    GACACAGACA TCTGTAATGC CATGGCCTGC CTAGGCGAGC CTGACCACAA CGAGGCGCTC
    TCAGTGGATG CTCGCCAGGA GCTGGACCTG CGAATCGGCT GTGCATTCAC CAGGTTTCAG
    ACTAAATATT TCCAGGGGAA ATACGGTGAT TTAGACAGCT CTCTCATCTC CTTTGGGCCG
    TGTCAGACTC CAACCCTGGG ATTCTGTGTG GAGAGACATG ATAAAATCCA GTCCTTCAAA
    CCAGAGACCT ACTGGGTGCT GCAGGCCAAG GTTAACACTG ACAAAGACAG ATCTCTCCTT
    TTGGACTGGG ACCGAGTAAG AGTGTTTGAC CGGGAGATCG CACAGATGTT TTTAAACATG
    ACAAAGCTGG AGAAGGAAGC CCAGGTGGAG GCCACAAGCA GGAAAGAAAA GGCCAAGCAG
    AGGCCCCTGG CCCTGAACAC TGTGGAGATG CTGCGTGTGG CCAGCTCTTC TCTGGGCATG
    GGGCCGCAGC ACGCCATGCA GACGGCTGAG CGGCTCTACA CGCAAGGCTA CATCAGCTAC
    CCACGGACAG AGACCACCCA CTACCCTGAG AACTTTGACC TGAAGGGCTC TCTGCGGCAG
    CAGGCCAACC ACCCCTACTG GGCCGACACG GTGAAGCGGT TGTTAGCAGA AGGTATCAAC
    CGCCCGCGGA AAGGCCATGA CGCCGGCGAC CATCCCCCCA TCACCCCCAT GAAGTCTGCC
    ACAGAGGCCG AATTAGGGGG TGACGCGTGG CGGCTCTATG AGTACATCAC CAGACACTTC
    ATCGCCACGG TCAGCCATGA CTGCAAGTAC CTGCAGAGCA CCATCTCCTT CAGAATTGGG
    CCCGAGCTCT TCACCTGCTC CGGGAAGACC GTCCTCTCAC CAGGCTTCAC GGAGGTCATG
    CCCTGGCAGA GCGTGCCCCT GGAGGAGAGC CTGCCCACTT GCCAGCGGGG TGATGCCTTC
    CCTGTGGGCG AGGTGAAGAT GCTGGAGAAG CAGACGAACC CACCCGACTA CCTGACGGAG
    GCCGAGCTCA TCACGCTCAT GGAGAAGCAT GGCATCGGCA CGGATGCCAG CATCCCTGTG
    CATATCAACA ACATCTGCCA GCGCAACTAT GTCACGGTGG AGAGCGGGCG CCGGCTCAAG
    CCCACCAACC TCGGCATCGT CCTGGTGCAC GGCTACTATA AGATTGATGC AGAGCTGGTG
    CTCCCCACCA TCCGCAGTGC AGTGGAGAAG CAGCTGAACC TGATCGCCCA GGGCAAGGCC
    GACTACCGCC AGGTCCTGGG CCACACCCTG GACGTGTTCA AGAGGAAGTT CCACTACTTT
    GTCGACTCCA TTGCTGGCAT GGATGAGTTG ATGGAGGTGT CTTTCTCGCC CCTGGCGGCC
    ACAGGCAAGC CCCTCTCACG CTGTGGGAAG TGCCACCGCT TCATGAAGTA CATCCAGGCC
    AAGCCAAGCC GCCTGCACTG CTCCCACTGC GATGAGACCT ACACGCTCCC CCAGAACGGC
    ACCATCAAGC TCTACAAGGA GCTCCGCTGC CCTCTGGATG ACTTCGAGCT GGTCCTGTGG
    TCATCAGGCT CTCGGGGCAA GAGCTACCCG CTGTGCCCCT ACTGCTACAA CCACCCACCC
    TTCCGAGACA TGAAGAAAGG CATGGGCTGC AACGAGTGTA CGCACCCCTC CTGCCAGCAC
    TCGCTGAGCA TGCTGGGCAT CGGCCAGTGC GTGGAATGTG AGAGCGGGGT GCTGGTGCTG
    GACCCCACCT CGGGCCCCAA GTGGAAGGTG GCCTGCAACA AGTGCAACGT GGTAGCGCAC
    TGCTTCGAGA ACGCCCACCG CGTGCGGGTG TCCGCCGACA CCTGCAGTGT CTGTGAGGCC
    GCCTTGCTTG ATGTGGACTT CAACAAGGCC AAGTCCCCAC TCCCGGGCGA TGAGACGCAG
    CACATGGGCT GCGTCTTTTG TGACCCCGTC TTCCAGGAGC TGGTGGAGCT GAAGCATGCG
    GCCTCCTGCC ACCCCATGCA CCGCGGTGGA CCAGGGAGAA GGCAGGGTCG AGGGCGGGGC
    CGGGCCAGGA GGCCCCCTGG GAAGCCCAAC CCCAGACGGC CCAAGGACAA GATGTCAGCC
    CTGGCCGCCT ACTTTGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336777.1
    CDS703..2883
    Translation

    Target ORF information:

    RefSeq Version NM_001349848.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349848.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAACAAGG CCCATGGTGG CGAGAAGACC GTGTTCCGGG CCAGGTTTAG CTCCATCACG 
    GACACAGACA TCTGTAATGC CATGGCCTGC CTAGGCGAGC CTGACCACAA CGAGGCGCTC
    TCAGTGGATG CTCGCCAGGA GCTGGACCTG CGAATCGGCT GTGCATTCAC CAGGTTTCAG
    ACTAAATATT TCCAGGGGAA ATACGGTGAT TTAGACAGCT CTCTCATCTC CTTTGGGCCG
    TGTCAGACTC CAACCCTGGG ATTCTGTGTG GAGAGACATG ATAAAATCCA GTCCTTCAAA
    CCAGAGACCT ACTGGGTGCT GCAGGCCAAG GTTAACACTG ACAAAGACAG ATCTCTCCTT
    TTGGACTGGG ACCGAGTAAG AGTGTTTGAC CGGGAGATCG CACAGATGTT TTTAAACATG
    ACAAAGCTGG AGAAGGAAGC CCAGGTGGAG GCCACAAGCA GGAAAGAAAA GGCCAAGCAG
    AGGCCCCTGG CCCTGAACAC TGTGGAGATG CTGCGTGTGG CCAGCTCTTC TCTGGGCATG
    GGGCCGCAGC ACGCCATGCA GACGGCTGAG CGGCTCTACA CGCAAGGCTA CATCAGCTAC
    CCACGGACAG AGACCACCCA CTACCCTGAG AACTTTGACC TGAAGGGCTC TCTGCGGCAG
    CAGGCCAACC ACCCCTACTG GGCCGACACG GTGAAGCGGT TGTTAGCAGA AGGTATCAAC
    CGCCCGCGGA AAGGCCATGA CGCCGGCGAC CATCCCCCCA TCACCCCCAT GAAGTCTGCC
    ACAGAGGCCG AATTAGGGGG TGACGCGTGG CGGCTCTATG AGTACATCAC CAGACACTTC
    ATCGCCACGG TCAGCCATGA CTGCAAGTAC CTGCAGAGCA CCATCTCCTT CAGAATTGGG
    CCCGAGCTCT TCACCTGCTC CGGGAAGACC GTCCTCTCAC CAGGCTTCAC GGAGGTCATG
    CCCTGGCAGA GCGTGCCCCT GGAGGAGAGC CTGCCCACTT GCCAGCGGGG TGATGCCTTC
    CCTGTGGGCG AGGTGAAGAT GCTGGAGAAG CAGACGAACC CACCCGACTA CCTGACGGAG
    GCCGAGCTCA TCACGCTCAT GGAGAAGCAT GGCATCGGCA CGGATGCCAG CATCCCTGTG
    CATATCAACA ACATCTGCCA GCGCAACTAT GTCACGGTGG AGAGCGGGCG CCGGCTCAAG
    CCCACCAACC TCGGCATCGT CCTGGTGCAC GGCTACTATA AGATTGATGC AGAGCTGGTG
    CTCCCCACCA TCCGCAGTGC AGTGGAGAAG CAGCTGAACC TGATCGCCCA GGGCAAGGCC
    GACTACCGCC AGGTCCTGGG CCACACCCTG GACGTGTTCA AGAGGAAGTT CCACTACTTT
    GTCGACTCCA TTGCTGGCAT GGATGAGTTG ATGGAGGTGT CTTTCTCGCC CCTGGCGGCC
    ACAGGCAAGC CCCTCTCACG CTGTGGGAAG TGCCACCGCT TCATGAAGTA CATCCAGGCC
    AAGCCAAGCC GCCTGCACTG CTCCCACTGC GATGAGACCT ACACGCTCCC CCAGAACGGC
    ACCATCAAGC TCTACAAGGA GCTCCGCTGC CCTCTGGATG ACTTCGAGCT GGTCCTGTGG
    TCATCAGGCT CTCGGGGCAA GAGCTACCCG CTGTGCCCCT ACTGCTACAA CCACCCACCC
    TTCCGAGACA TGAAGAAAGG CATGGGCTGC AACGAGTGTA CGCACCCCTC CTGCCAGCAC
    TCGCTGAGCA TGCTGGGCAT CGGCCAGTGC GTGGAATGTG AGAGCGGGGT GCTGGTGCTG
    GACCCCACCT CGGGCCCCAA GTGGAAGGTG GCCTGCAACA AGTGCAACGT GGTAGCGCAC
    TGCTTCGAGA ACGCCCACCG CGTGCGGGTG TCCGCCGACA CCTGCAGTGT CTGTGAGGCC
    GCCTTGCTTG ATGTGGACTT CAACAAGGCC AAGTCCCCAC TCCCGGGCGA TGAGACGCAG
    CACATGGGCT GCGTCTTTTG TGACCCCGTC TTCCAGGAGC TGGTGGAGCT GAAGCATGCG
    GCCTCCTGCC ACCCCATGCA CCGCGGTGGA CCAGGGAGAA GGCAGGGTCG AGGGCGGGGC
    CGGGCCAGGA GGCCCCCTGG GAAGCCCAAC CCCAGACGGC CCAAGGACAA GATGTCAGCC
    CTGGCCGCCT ACTTTGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53736
    Clone ID Related Accession (Same CDS sequence) NM_001349850.2 , NM_001349848.1 , NM_001349848.2 , NM_001349850.1
    Accession Version NM_001349848.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2181bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-10
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-beta-1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA025312.1, DB165571.1, DR003213.1, AK290997.1, AC245452.3 and BQ270362.1. On Jun 2, 2019 this sequence version replaced NM_001349848.1. Transcript Variant: This variant (6) uses an alternate splice site in its 5' terminal exon and contains an alternate exon in its 5' UTR, resulting in the use of a downstream start codon compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. Variants 6 and 7 encode the same protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.152412.1, SRR1660809.53960.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158569 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAACAAGG CCCATGGTGG CGAGAAGACC GTGTTCCGGG CCAGGTTTAG CTCCATCACG 
    GACACAGACA TCTGTAATGC CATGGCCTGC CTAGGCGAGC CTGACCACAA CGAGGCGCTC
    TCAGTGGATG CTCGCCAGGA GCTGGACCTG CGAATCGGCT GTGCATTCAC CAGGTTTCAG
    ACTAAATATT TCCAGGGGAA ATACGGTGAT TTAGACAGCT CTCTCATCTC CTTTGGGCCG
    TGTCAGACTC CAACCCTGGG ATTCTGTGTG GAGAGACATG ATAAAATCCA GTCCTTCAAA
    CCAGAGACCT ACTGGGTGCT GCAGGCCAAG GTTAACACTG ACAAAGACAG ATCTCTCCTT
    TTGGACTGGG ACCGAGTAAG AGTGTTTGAC CGGGAGATCG CACAGATGTT TTTAAACATG
    ACAAAGCTGG AGAAGGAAGC CCAGGTGGAG GCCACAAGCA GGAAAGAAAA GGCCAAGCAG
    AGGCCCCTGG CCCTGAACAC TGTGGAGATG CTGCGTGTGG CCAGCTCTTC TCTGGGCATG
    GGGCCGCAGC ACGCCATGCA GACGGCTGAG CGGCTCTACA CGCAAGGCTA CATCAGCTAC
    CCACGGACAG AGACCACCCA CTACCCTGAG AACTTTGACC TGAAGGGCTC TCTGCGGCAG
    CAGGCCAACC ACCCCTACTG GGCCGACACG GTGAAGCGGT TGTTAGCAGA AGGTATCAAC
    CGCCCGCGGA AAGGCCATGA CGCCGGCGAC CATCCCCCCA TCACCCCCAT GAAGTCTGCC
    ACAGAGGCCG AATTAGGGGG TGACGCGTGG CGGCTCTATG AGTACATCAC CAGACACTTC
    ATCGCCACGG TCAGCCATGA CTGCAAGTAC CTGCAGAGCA CCATCTCCTT CAGAATTGGG
    CCCGAGCTCT TCACCTGCTC CGGGAAGACC GTCCTCTCAC CAGGCTTCAC GGAGGTCATG
    CCCTGGCAGA GCGTGCCCCT GGAGGAGAGC CTGCCCACTT GCCAGCGGGG TGATGCCTTC
    CCTGTGGGCG AGGTGAAGAT GCTGGAGAAG CAGACGAACC CACCCGACTA CCTGACGGAG
    GCCGAGCTCA TCACGCTCAT GGAGAAGCAT GGCATCGGCA CGGATGCCAG CATCCCTGTG
    CATATCAACA ACATCTGCCA GCGCAACTAT GTCACGGTGG AGAGCGGGCG CCGGCTCAAG
    CCCACCAACC TCGGCATCGT CCTGGTGCAC GGCTACTATA AGATTGATGC AGAGCTGGTG
    CTCCCCACCA TCCGCAGTGC AGTGGAGAAG CAGCTGAACC TGATCGCCCA GGGCAAGGCC
    GACTACCGCC AGGTCCTGGG CCACACCCTG GACGTGTTCA AGAGGAAGTT CCACTACTTT
    GTCGACTCCA TTGCTGGCAT GGATGAGTTG ATGGAGGTGT CTTTCTCGCC CCTGGCGGCC
    ACAGGCAAGC CCCTCTCACG CTGTGGGAAG TGCCACCGCT TCATGAAGTA CATCCAGGCC
    AAGCCAAGCC GCCTGCACTG CTCCCACTGC GATGAGACCT ACACGCTCCC CCAGAACGGC
    ACCATCAAGC TCTACAAGGA GCTCCGCTGC CCTCTGGATG ACTTCGAGCT GGTCCTGTGG
    TCATCAGGCT CTCGGGGCAA GAGCTACCCG CTGTGCCCCT ACTGCTACAA CCACCCACCC
    TTCCGAGACA TGAAGAAAGG CATGGGCTGC AACGAGTGTA CGCACCCCTC CTGCCAGCAC
    TCGCTGAGCA TGCTGGGCAT CGGCCAGTGC GTGGAATGTG AGAGCGGGGT GCTGGTGCTG
    GACCCCACCT CGGGCCCCAA GTGGAAGGTG GCCTGCAACA AGTGCAACGT GGTAGCGCAC
    TGCTTCGAGA ACGCCCACCG CGTGCGGGTG TCCGCCGACA CCTGCAGTGT CTGTGAGGCC
    GCCTTGCTTG ATGTGGACTT CAACAAGGCC AAGTCCCCAC TCCCGGGCGA TGAGACGCAG
    CACATGGGCT GCGTCTTTTG TGACCCCGTC TTCCAGGAGC TGGTGGAGCT GAAGCATGCG
    GCCTCCTGCC ACCCCATGCA CCGCGGTGGA CCAGGGAGAA GGCAGGGTCG AGGGCGGGGC
    CGGGCCAGGA GGCCCCCTGG GAAGCCCAAC CCCAGACGGC CCAAGGACAA GATGTCAGCC
    CTGGCCGCCT ACTTTGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336777.1
    CDS647..2827
    Translation

    Target ORF information:

    RefSeq Version NM_001349848.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349848.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAACAAGG CCCATGGTGG CGAGAAGACC GTGTTCCGGG CCAGGTTTAG CTCCATCACG 
    GACACAGACA TCTGTAATGC CATGGCCTGC CTAGGCGAGC CTGACCACAA CGAGGCGCTC
    TCAGTGGATG CTCGCCAGGA GCTGGACCTG CGAATCGGCT GTGCATTCAC CAGGTTTCAG
    ACTAAATATT TCCAGGGGAA ATACGGTGAT TTAGACAGCT CTCTCATCTC CTTTGGGCCG
    TGTCAGACTC CAACCCTGGG ATTCTGTGTG GAGAGACATG ATAAAATCCA GTCCTTCAAA
    CCAGAGACCT ACTGGGTGCT GCAGGCCAAG GTTAACACTG ACAAAGACAG ATCTCTCCTT
    TTGGACTGGG ACCGAGTAAG AGTGTTTGAC CGGGAGATCG CACAGATGTT TTTAAACATG
    ACAAAGCTGG AGAAGGAAGC CCAGGTGGAG GCCACAAGCA GGAAAGAAAA GGCCAAGCAG
    AGGCCCCTGG CCCTGAACAC TGTGGAGATG CTGCGTGTGG CCAGCTCTTC TCTGGGCATG
    GGGCCGCAGC ACGCCATGCA GACGGCTGAG CGGCTCTACA CGCAAGGCTA CATCAGCTAC
    CCACGGACAG AGACCACCCA CTACCCTGAG AACTTTGACC TGAAGGGCTC TCTGCGGCAG
    CAGGCCAACC ACCCCTACTG GGCCGACACG GTGAAGCGGT TGTTAGCAGA AGGTATCAAC
    CGCCCGCGGA AAGGCCATGA CGCCGGCGAC CATCCCCCCA TCACCCCCAT GAAGTCTGCC
    ACAGAGGCCG AATTAGGGGG TGACGCGTGG CGGCTCTATG AGTACATCAC CAGACACTTC
    ATCGCCACGG TCAGCCATGA CTGCAAGTAC CTGCAGAGCA CCATCTCCTT CAGAATTGGG
    CCCGAGCTCT TCACCTGCTC CGGGAAGACC GTCCTCTCAC CAGGCTTCAC GGAGGTCATG
    CCCTGGCAGA GCGTGCCCCT GGAGGAGAGC CTGCCCACTT GCCAGCGGGG TGATGCCTTC
    CCTGTGGGCG AGGTGAAGAT GCTGGAGAAG CAGACGAACC CACCCGACTA CCTGACGGAG
    GCCGAGCTCA TCACGCTCAT GGAGAAGCAT GGCATCGGCA CGGATGCCAG CATCCCTGTG
    CATATCAACA ACATCTGCCA GCGCAACTAT GTCACGGTGG AGAGCGGGCG CCGGCTCAAG
    CCCACCAACC TCGGCATCGT CCTGGTGCAC GGCTACTATA AGATTGATGC AGAGCTGGTG
    CTCCCCACCA TCCGCAGTGC AGTGGAGAAG CAGCTGAACC TGATCGCCCA GGGCAAGGCC
    GACTACCGCC AGGTCCTGGG CCACACCCTG GACGTGTTCA AGAGGAAGTT CCACTACTTT
    GTCGACTCCA TTGCTGGCAT GGATGAGTTG ATGGAGGTGT CTTTCTCGCC CCTGGCGGCC
    ACAGGCAAGC CCCTCTCACG CTGTGGGAAG TGCCACCGCT TCATGAAGTA CATCCAGGCC
    AAGCCAAGCC GCCTGCACTG CTCCCACTGC GATGAGACCT ACACGCTCCC CCAGAACGGC
    ACCATCAAGC TCTACAAGGA GCTCCGCTGC CCTCTGGATG ACTTCGAGCT GGTCCTGTGG
    TCATCAGGCT CTCGGGGCAA GAGCTACCCG CTGTGCCCCT ACTGCTACAA CCACCCACCC
    TTCCGAGACA TGAAGAAAGG CATGGGCTGC AACGAGTGTA CGCACCCCTC CTGCCAGCAC
    TCGCTGAGCA TGCTGGGCAT CGGCCAGTGC GTGGAATGTG AGAGCGGGGT GCTGGTGCTG
    GACCCCACCT CGGGCCCCAA GTGGAAGGTG GCCTGCAACA AGTGCAACGT GGTAGCGCAC
    TGCTTCGAGA ACGCCCACCG CGTGCGGGTG TCCGCCGACA CCTGCAGTGT CTGTGAGGCC
    GCCTTGCTTG ATGTGGACTT CAACAAGGCC AAGTCCCCAC TCCCGGGCGA TGAGACGCAG
    CACATGGGCT GCGTCTTTTG TGACCCCGTC TTCCAGGAGC TGGTGGAGCT GAAGCATGCG
    GCCTCCTGCC ACCCCATGCA CCGCGGTGGA CCAGGGAGAA GGCAGGGTCG AGGGCGGGGC
    CGGGCCAGGA GGCCCCCTGG GAAGCCCAAC CCCAGACGGC CCAAGGACAA GATGTCAGCC
    CTGGCCGCCT ACTTTGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53736
    Clone ID Related Accession (Same CDS sequence) NM_001349850.2 , NM_001349848.1 , NM_001349848.2 , NM_001349850.1
    Accession Version NM_001349850.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2181bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-03
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-beta-1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA025312.1, DA124773.1, DB146985.1, DB150453.1, AK290997.1, AC245452.3 and BQ270362.1. On Mar 28, 2017 this sequence version replaced XM_017029039.1. Transcript Variant: This variant (7) contains an alternate exon structure in the 5' region and contains an alternate exon in its 5' UTR, resulting in the use of a downstream start codon compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. Variants 6 and 7 encode the same protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.246203.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAACAAGG CCCATGGTGG CGAGAAGACC GTGTTCCGGG CCAGGTTTAG CTCCATCACG 
    GACACAGACA TCTGTAATGC CATGGCCTGC CTAGGCGAGC CTGACCACAA CGAGGCGCTC
    TCAGTGGATG CTCGCCAGGA GCTGGACCTG CGAATCGGCT GTGCATTCAC CAGGTTTCAG
    ACTAAATATT TCCAGGGGAA ATACGGTGAT TTAGACAGCT CTCTCATCTC CTTTGGGCCG
    TGTCAGACTC CAACCCTGGG ATTCTGTGTG GAGAGACATG ATAAAATCCA GTCCTTCAAA
    CCAGAGACCT ACTGGGTGCT GCAGGCCAAG GTTAACACTG ACAAAGACAG ATCTCTCCTT
    TTGGACTGGG ACCGAGTAAG AGTGTTTGAC CGGGAGATCG CACAGATGTT TTTAAACATG
    ACAAAGCTGG AGAAGGAAGC CCAGGTGGAG GCCACAAGCA GGAAAGAAAA GGCCAAGCAG
    AGGCCCCTGG CCCTGAACAC TGTGGAGATG CTGCGTGTGG CCAGCTCTTC TCTGGGCATG
    GGGCCGCAGC ACGCCATGCA GACGGCTGAG CGGCTCTACA CGCAAGGCTA CATCAGCTAC
    CCACGGACAG AGACCACCCA CTACCCTGAG AACTTTGACC TGAAGGGCTC TCTGCGGCAG
    CAGGCCAACC ACCCCTACTG GGCCGACACG GTGAAGCGGT TGTTAGCAGA AGGTATCAAC
    CGCCCGCGGA AAGGCCATGA CGCCGGCGAC CATCCCCCCA TCACCCCCAT GAAGTCTGCC
    ACAGAGGCCG AATTAGGGGG TGACGCGTGG CGGCTCTATG AGTACATCAC CAGACACTTC
    ATCGCCACGG TCAGCCATGA CTGCAAGTAC CTGCAGAGCA CCATCTCCTT CAGAATTGGG
    CCCGAGCTCT TCACCTGCTC CGGGAAGACC GTCCTCTCAC CAGGCTTCAC GGAGGTCATG
    CCCTGGCAGA GCGTGCCCCT GGAGGAGAGC CTGCCCACTT GCCAGCGGGG TGATGCCTTC
    CCTGTGGGCG AGGTGAAGAT GCTGGAGAAG CAGACGAACC CACCCGACTA CCTGACGGAG
    GCCGAGCTCA TCACGCTCAT GGAGAAGCAT GGCATCGGCA CGGATGCCAG CATCCCTGTG
    CATATCAACA ACATCTGCCA GCGCAACTAT GTCACGGTGG AGAGCGGGCG CCGGCTCAAG
    CCCACCAACC TCGGCATCGT CCTGGTGCAC GGCTACTATA AGATTGATGC AGAGCTGGTG
    CTCCCCACCA TCCGCAGTGC AGTGGAGAAG CAGCTGAACC TGATCGCCCA GGGCAAGGCC
    GACTACCGCC AGGTCCTGGG CCACACCCTG GACGTGTTCA AGAGGAAGTT CCACTACTTT
    GTCGACTCCA TTGCTGGCAT GGATGAGTTG ATGGAGGTGT CTTTCTCGCC CCTGGCGGCC
    ACAGGCAAGC CCCTCTCACG CTGTGGGAAG TGCCACCGCT TCATGAAGTA CATCCAGGCC
    AAGCCAAGCC GCCTGCACTG CTCCCACTGC GATGAGACCT ACACGCTCCC CCAGAACGGC
    ACCATCAAGC TCTACAAGGA GCTCCGCTGC CCTCTGGATG ACTTCGAGCT GGTCCTGTGG
    TCATCAGGCT CTCGGGGCAA GAGCTACCCG CTGTGCCCCT ACTGCTACAA CCACCCACCC
    TTCCGAGACA TGAAGAAAGG CATGGGCTGC AACGAGTGTA CGCACCCCTC CTGCCAGCAC
    TCGCTGAGCA TGCTGGGCAT CGGCCAGTGC GTGGAATGTG AGAGCGGGGT GCTGGTGCTG
    GACCCCACCT CGGGCCCCAA GTGGAAGGTG GCCTGCAACA AGTGCAACGT GGTAGCGCAC
    TGCTTCGAGA ACGCCCACCG CGTGCGGGTG TCCGCCGACA CCTGCAGTGT CTGTGAGGCC
    GCCTTGCTTG ATGTGGACTT CAACAAGGCC AAGTCCCCAC TCCCGGGCGA TGAGACGCAG
    CACATGGGCT GCGTCTTTTG TGACCCCGTC TTCCAGGAGC TGGTGGAGCT GAAGCATGCG
    GCCTCCTGCC ACCCCATGCA CCGCGGTGGA CCAGGGAGAA GGCAGGGTCG AGGGCGGGGC
    CGGGCCAGGA GGCCCCCTGG GAAGCCCAAC CCCAGACGGC CCAAGGACAA GATGTCAGCC
    CTGGCCGCCT ACTTTGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336779.1
    CDS739..2919
    Translation

    Target ORF information:

    RefSeq Version NM_001349850.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349850.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAACAAGG CCCATGGTGG CGAGAAGACC GTGTTCCGGG CCAGGTTTAG CTCCATCACG 
    GACACAGACA TCTGTAATGC CATGGCCTGC CTAGGCGAGC CTGACCACAA CGAGGCGCTC
    TCAGTGGATG CTCGCCAGGA GCTGGACCTG CGAATCGGCT GTGCATTCAC CAGGTTTCAG
    ACTAAATATT TCCAGGGGAA ATACGGTGAT TTAGACAGCT CTCTCATCTC CTTTGGGCCG
    TGTCAGACTC CAACCCTGGG ATTCTGTGTG GAGAGACATG ATAAAATCCA GTCCTTCAAA
    CCAGAGACCT ACTGGGTGCT GCAGGCCAAG GTTAACACTG ACAAAGACAG ATCTCTCCTT
    TTGGACTGGG ACCGAGTAAG AGTGTTTGAC CGGGAGATCG CACAGATGTT TTTAAACATG
    ACAAAGCTGG AGAAGGAAGC CCAGGTGGAG GCCACAAGCA GGAAAGAAAA GGCCAAGCAG
    AGGCCCCTGG CCCTGAACAC TGTGGAGATG CTGCGTGTGG CCAGCTCTTC TCTGGGCATG
    GGGCCGCAGC ACGCCATGCA GACGGCTGAG CGGCTCTACA CGCAAGGCTA CATCAGCTAC
    CCACGGACAG AGACCACCCA CTACCCTGAG AACTTTGACC TGAAGGGCTC TCTGCGGCAG
    CAGGCCAACC ACCCCTACTG GGCCGACACG GTGAAGCGGT TGTTAGCAGA AGGTATCAAC
    CGCCCGCGGA AAGGCCATGA CGCCGGCGAC CATCCCCCCA TCACCCCCAT GAAGTCTGCC
    ACAGAGGCCG AATTAGGGGG TGACGCGTGG CGGCTCTATG AGTACATCAC CAGACACTTC
    ATCGCCACGG TCAGCCATGA CTGCAAGTAC CTGCAGAGCA CCATCTCCTT CAGAATTGGG
    CCCGAGCTCT TCACCTGCTC CGGGAAGACC GTCCTCTCAC CAGGCTTCAC GGAGGTCATG
    CCCTGGCAGA GCGTGCCCCT GGAGGAGAGC CTGCCCACTT GCCAGCGGGG TGATGCCTTC
    CCTGTGGGCG AGGTGAAGAT GCTGGAGAAG CAGACGAACC CACCCGACTA CCTGACGGAG
    GCCGAGCTCA TCACGCTCAT GGAGAAGCAT GGCATCGGCA CGGATGCCAG CATCCCTGTG
    CATATCAACA ACATCTGCCA GCGCAACTAT GTCACGGTGG AGAGCGGGCG CCGGCTCAAG
    CCCACCAACC TCGGCATCGT CCTGGTGCAC GGCTACTATA AGATTGATGC AGAGCTGGTG
    CTCCCCACCA TCCGCAGTGC AGTGGAGAAG CAGCTGAACC TGATCGCCCA GGGCAAGGCC
    GACTACCGCC AGGTCCTGGG CCACACCCTG GACGTGTTCA AGAGGAAGTT CCACTACTTT
    GTCGACTCCA TTGCTGGCAT GGATGAGTTG ATGGAGGTGT CTTTCTCGCC CCTGGCGGCC
    ACAGGCAAGC CCCTCTCACG CTGTGGGAAG TGCCACCGCT TCATGAAGTA CATCCAGGCC
    AAGCCAAGCC GCCTGCACTG CTCCCACTGC GATGAGACCT ACACGCTCCC CCAGAACGGC
    ACCATCAAGC TCTACAAGGA GCTCCGCTGC CCTCTGGATG ACTTCGAGCT GGTCCTGTGG
    TCATCAGGCT CTCGGGGCAA GAGCTACCCG CTGTGCCCCT ACTGCTACAA CCACCCACCC
    TTCCGAGACA TGAAGAAAGG CATGGGCTGC AACGAGTGTA CGCACCCCTC CTGCCAGCAC
    TCGCTGAGCA TGCTGGGCAT CGGCCAGTGC GTGGAATGTG AGAGCGGGGT GCTGGTGCTG
    GACCCCACCT CGGGCCCCAA GTGGAAGGTG GCCTGCAACA AGTGCAACGT GGTAGCGCAC
    TGCTTCGAGA ACGCCCACCG CGTGCGGGTG TCCGCCGACA CCTGCAGTGT CTGTGAGGCC
    GCCTTGCTTG ATGTGGACTT CAACAAGGCC AAGTCCCCAC TCCCGGGCGA TGAGACGCAG
    CACATGGGCT GCGTCTTTTG TGACCCCGTC TTCCAGGAGC TGGTGGAGCT GAAGCATGCG
    GCCTCCTGCC ACCCCATGCA CCGCGGTGGA CCAGGGAGAA GGCAGGGTCG AGGGCGGGGC
    CGGGCCAGGA GGCCCCCTGG GAAGCCCAAC CCCAGACGGC CCAAGGACAA GATGTCAGCC
    CTGGCCGCCT ACTTTGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23269
    Clone ID Related Accession (Same CDS sequence) NM_001282112.1 , NM_001349845.1 , NM_001282113.1 , XM_005261811.1 , NM_001349847.1 , NM_003935.4 , NM_001282113.2 , NM_001349847.2 , XM_024452297.1 , XM_011530482.1 , NM_003935.5 , NM_001349845.2 , NM_001282112.2
    Accession Version XM_005261811.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-beta-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGGCA TGGGCTGCAA CGAGTGTACG CACCCCTCCT GCCAGCACTC GCTGAGCATG
    CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGGGTGC TGGTGCTGGA CCCCACCTCG
    GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TAGCGCACTG CTTCGAGAAC
    GCCCACCGCG TGCGGGTGTC CGCCGACACC TGCAGTGTCT GTGAGGCCGC CTTGCTTGAT
    GTGGACTTCA ACAAGGCCAA GTCCCCACTC CCGGGCGATG AGACGCAGCA CATGGGCTGC
    GTCTTTTGTG ACCCCGTCTT CCAGGAGCTG GTGGAGCTGA AGCATGCGGC CTCCTGCCAC
    CCCATGCACC GCGGTGGACC AGGGAGAAGG CAGGGTCGAG GGCGGGGCCG GGCCAGGAGG
    CCCCCTGGGA AGCCCAACCC CAGACGGCCC AAGGACAAGA TGTCAGCCCT GGCCGCCTAC
    TTTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005261868.1
    CDS379..2967
    Translation

    Target ORF information:

    RefSeq Version XM_005261811.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005261811.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGGCA TGGGCTGCAA CGAGTGTACG CACCCCTCCT GCCAGCACTC GCTGAGCATG
    CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGGGTGC TGGTGCTGGA CCCCACCTCG
    GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TAGCGCACTG CTTCGAGAAC
    GCCCACCGCG TGCGGGTGTC CGCCGACACC TGCAGTGTCT GTGAGGCCGC CTTGCTTGAT
    GTGGACTTCA ACAAGGCCAA GTCCCCACTC CCGGGCGATG AGACGCAGCA CATGGGCTGC
    GTCTTTTGTG ACCCCGTCTT CCAGGAGCTG GTGGAGCTGA AGCATGCGGC CTCCTGCCAC
    CCCATGCACC GCGGTGGACC AGGGAGAAGG CAGGGTCGAG GGCGGGGCCG GGCCAGGAGG
    CCCCCTGGGA AGCCCAACCC CAGACGGCCC AAGGACAAGA TGTCAGCCCT GGCCGCCTAC
    TTTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23269
    Clone ID Related Accession (Same CDS sequence) NM_001282112.1 , NM_001349845.1 , NM_001282113.1 , XM_005261811.1 , NM_001349847.1 , NM_003935.4 , NM_001282113.2 , NM_001349847.2 , XM_024452297.1 , XM_011530482.1 , NM_003935.5 , NM_001349845.2 , NM_001282112.2
    Accession Version XM_024452297.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-beta-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGGCA TGGGCTGCAA CGAGTGTACG CACCCCTCCT GCCAGCACTC GCTGAGCATG
    CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGGGTGC TGGTGCTGGA CCCCACCTCG
    GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TAGCGCACTG CTTCGAGAAC
    GCCCACCGCG TGCGGGTGTC CGCCGACACC TGCAGTGTCT GTGAGGCCGC CTTGCTTGAT
    GTGGACTTCA ACAAGGCCAA GTCCCCACTC CCGGGCGATG AGACGCAGCA CATGGGCTGC
    GTCTTTTGTG ACCCCGTCTT CCAGGAGCTG GTGGAGCTGA AGCATGCGGC CTCCTGCCAC
    CCCATGCACC GCGGTGGACC AGGGAGAAGG CAGGGTCGAG GGCGGGGCCG GGCCAGGAGG
    CCCCCTGGGA AGCCCAACCC CAGACGGCCC AAGGACAAGA TGTCAGCCCT GGCCGCCTAC
    TTTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024308065.1
    CDS1452..4040
    Translation

    Target ORF information:

    RefSeq Version XM_024452297.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024452297.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGGCA TGGGCTGCAA CGAGTGTACG CACCCCTCCT GCCAGCACTC GCTGAGCATG
    CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGGGTGC TGGTGCTGGA CCCCACCTCG
    GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TAGCGCACTG CTTCGAGAAC
    GCCCACCGCG TGCGGGTGTC CGCCGACACC TGCAGTGTCT GTGAGGCCGC CTTGCTTGAT
    GTGGACTTCA ACAAGGCCAA GTCCCCACTC CCGGGCGATG AGACGCAGCA CATGGGCTGC
    GTCTTTTGTG ACCCCGTCTT CCAGGAGCTG GTGGAGCTGA AGCATGCGGC CTCCTGCCAC
    CCCATGCACC GCGGTGGACC AGGGAGAAGG CAGGGTCGAG GGCGGGGCCG GGCCAGGAGG
    CCCCCTGGGA AGCCCAACCC CAGACGGCCC AAGGACAAGA TGTCAGCCCT GGCCGCCTAC
    TTTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23269
    Clone ID Related Accession (Same CDS sequence) NM_001282112.1 , NM_001349845.1 , NM_001282113.1 , XM_005261811.1 , NM_001349847.1 , NM_003935.4 , NM_001282113.2 , NM_001349847.2 , XM_024452297.1 , XM_011530482.1 , NM_003935.5 , NM_001349845.2 , NM_001282112.2
    Accession Version XM_011530482.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-beta-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGGCA TGGGCTGCAA CGAGTGTACG CACCCCTCCT GCCAGCACTC GCTGAGCATG
    CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGGGTGC TGGTGCTGGA CCCCACCTCG
    GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TAGCGCACTG CTTCGAGAAC
    GCCCACCGCG TGCGGGTGTC CGCCGACACC TGCAGTGTCT GTGAGGCCGC CTTGCTTGAT
    GTGGACTTCA ACAAGGCCAA GTCCCCACTC CCGGGCGATG AGACGCAGCA CATGGGCTGC
    GTCTTTTGTG ACCCCGTCTT CCAGGAGCTG GTGGAGCTGA AGCATGCGGC CTCCTGCCAC
    CCCATGCACC GCGGTGGACC AGGGAGAAGG CAGGGTCGAG GGCGGGGCCG GGCCAGGAGG
    CCCCCTGGGA AGCCCAACCC CAGACGGCCC AAGGACAAGA TGTCAGCCCT GGCCGCCTAC
    TTTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528784.1
    CDS446..3034
    Translation

    Target ORF information:

    RefSeq Version XM_011530482.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530482.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGGCA TGGGCTGCAA CGAGTGTACG CACCCCTCCT GCCAGCACTC GCTGAGCATG
    CTGGGCATCG GCCAGTGCGT GGAATGTGAG AGCGGGGTGC TGGTGCTGGA CCCCACCTCG
    GGCCCCAAGT GGAAGGTGGC CTGCAACAAG TGCAACGTGG TAGCGCACTG CTTCGAGAAC
    GCCCACCGCG TGCGGGTGTC CGCCGACACC TGCAGTGTCT GTGAGGCCGC CTTGCTTGAT
    GTGGACTTCA ACAAGGCCAA GTCCCCACTC CCGGGCGATG AGACGCAGCA CATGGGCTGC
    GTCTTTTGTG ACCCCGTCTT CCAGGAGCTG GTGGAGCTGA AGCATGCGGC CTCCTGCCAC
    CCCATGCACC GCGGTGGACC AGGGAGAAGG CAGGGTCGAG GGCGGGGCCG GGCCAGGAGG
    CCCCCTGGGA AGCCCAACCC CAGACGGCCC AAGGACAAGA TGTCAGCCCT GGCCGCCTAC
    TTTGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102339
    Clone ID Related Accession (Same CDS sequence) NM_001349852.1 , NM_001349851.2 , NM_001349851.1 , NM_001349852.2
    Accession Version NM_001349852.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-03
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-beta-1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA025312.1, DA124773.1, DB146985.1, DB150453.1, DR003213.1, AI361851.1, CN353232.1, CN302239.1, CR980357.1, CF137541.1, CN353231.1, CT002728.1, AK290997.1 and BQ270362.1. On Mar 28, 2017 this sequence version replaced XM_017029041.1. Transcript Variant: This variant (9) differs in its 5' UTR, resulting in the use of a downstream start codon, and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (3) has a shorter N-terminus and a shorter and distinct C-terminus comparted to isoform 1. Variants 8 and 9 encode the same protein (isoform 3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.180278.1, SRR1660805.20507.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAACAAGG CCCATGGTGG CGAGAAGACC GTGTTCCGGG CCAGGTTTAG CTCCATCACG 
    GACACAGACA TCTGTAATGC CATGGCCTGC CTAGGCGAGC CTGACCACAA CGAGGCGCTC
    TCAGTGGATG CTCGCCAGGA GCTGGACCTG CGAATCGGCT GTGCATTCAC CAGGTTTCAG
    ACTAAATATT TCCAGGGGAA ATACGGTGAT TTAGACAGCT CTCTCATCTC CTTTGGGCCG
    TGTCAGACTC CAACCCTGGG ATTCTGTGTG GAGAGACATG ATAAAATCCA GTCCTTCAAA
    CCAGAGACCT ACTGGGTGCT GCAGGCCAAG GTTAACACTG ACAAAGACAG ATCTCTCCTT
    TTGGACTGGG ACCGAGTAAG AGTGTTTGAC CGGGAGATCG CACAGATGTT TTTAAACATG
    ACAAAGCTGG AGAAGGAAGC CCAGGTGGAG GCCACAAGCA GGAAAGAAAA GGCCAAGCAG
    AGGCCCCTGG CCCTGAACAC TGTGGAGATG CTGCGTGTGG CCAGCTCTTC TCTGGGCATG
    GGGCCGCAGC ACGCCATGCA GACGGCTGAG CGGCTCTACA CGCAAGGCTA CATCAGCTAC
    CCACGGACAG AGACCACCCA CTACCCTGAG AACTTTGACC TGAAGGGCTC TCTGCGGCAG
    CAGGCCAACC ACCCCTACTG GGCCGACACG GTGAAGCGGT TGTTAGCAGA AGGTATCAAC
    CGCCCGCGGA AAGGCCATGA CGCCGGCGAC CATCCCCCCA TCACCCCCAT GAAGTCTGCC
    ACAGAGGCCG AATTAGGGGG TGACGCGTGG CGGCTCTATG AGTACATCAC CAGACACTTC
    ATCGCCACGG TCAGCCATGA CTGCAAGTAC CTGCAGAGCA CCATCTCCTT CAGAATTGGG
    CCCGAGCTCT TCACCTGCTC CGGGAAGACC GTCCTCTCAC CAGGCTTCAC GGAGGTCATG
    CCCTGGCAGA GCGTGCCCCT GGAGGAGAGC CTGCCCACTT GCCAGCGGGG TGATGCCTTC
    CCTGTGGGCG AGGTGAAGAT GCTGGAGAAG CAGACGAACC CACCCGACTA CCTGACGGAG
    GCCGAGCTCA TCACGCTCAT GGAGAAGCAT GGCATCGGCA CGGATGCCAG CATCCCTGTG
    CATATCAACA ACATCTGCCA GCGCAACTAT GTCACGGTGG AGAGCGGGCG CCGGCTCAAG
    CCCACCAACC TCGGCATCGT CCTGGTGCAC GGCTACTATA AGATTGATGC AGAGCTGGTG
    CTCCCCACCA TCCGCAGTGC AGTGGAGAAG CAGCTGAACC TGATCGCCCA GGGCAAGGCC
    GACTACCGCC AGGTCCTGGG CCACACCCTG GACGTGTTCA AGAGGAAGTT CCACTACTTT
    GTCGACTCCA TTGCTGGCAT GGATGAGTTG ATGGAGGTGT CTTTCTCGCC CCTGGCGGCC
    ACAGGCAAGC CCCTCTCACG CTGTGGGAAG TGCCACCGCT TCATGAAGTA CATCCAGGCC
    AAGCCAAGCC GCCTGCACTG CTCCCACTGC GATGAGACCT ACACGCTCCC CCAGAACGGC
    ACCATCAAGC TCTACAAGGA GCTCCGCTGC CCTCTGGATG ACTTCGAGCT GGTCCTGTGG
    TCATCAGGCT CTCGGGGCAA GAGCTACCCG CTGTGCCCCT ACTGCTACAA CCACCCACCC
    TTCCGAGACA TGAAGAAAGG TGAGTGCAGC CACTCCCTCC TGTCCACAGG TAGCTGCAGT
    CTCTTTTCAG TGCCAACCCC TGCCTTGCAC CAGGCTGGAC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336781.1
    CDS739..2523
    Translation

    Target ORF information:

    RefSeq Version NM_001349852.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349852.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAACAAGG CCCATGGTGG CGAGAAGACC GTGTTCCGGG CCAGGTTTAG CTCCATCACG 
    GACACAGACA TCTGTAATGC CATGGCCTGC CTAGGCGAGC CTGACCACAA CGAGGCGCTC
    TCAGTGGATG CTCGCCAGGA GCTGGACCTG CGAATCGGCT GTGCATTCAC CAGGTTTCAG
    ACTAAATATT TCCAGGGGAA ATACGGTGAT TTAGACAGCT CTCTCATCTC CTTTGGGCCG
    TGTCAGACTC CAACCCTGGG ATTCTGTGTG GAGAGACATG ATAAAATCCA GTCCTTCAAA
    CCAGAGACCT ACTGGGTGCT GCAGGCCAAG GTTAACACTG ACAAAGACAG ATCTCTCCTT
    TTGGACTGGG ACCGAGTAAG AGTGTTTGAC CGGGAGATCG CACAGATGTT TTTAAACATG
    ACAAAGCTGG AGAAGGAAGC CCAGGTGGAG GCCACAAGCA GGAAAGAAAA GGCCAAGCAG
    AGGCCCCTGG CCCTGAACAC TGTGGAGATG CTGCGTGTGG CCAGCTCTTC TCTGGGCATG
    GGGCCGCAGC ACGCCATGCA GACGGCTGAG CGGCTCTACA CGCAAGGCTA CATCAGCTAC
    CCACGGACAG AGACCACCCA CTACCCTGAG AACTTTGACC TGAAGGGCTC TCTGCGGCAG
    CAGGCCAACC ACCCCTACTG GGCCGACACG GTGAAGCGGT TGTTAGCAGA AGGTATCAAC
    CGCCCGCGGA AAGGCCATGA CGCCGGCGAC CATCCCCCCA TCACCCCCAT GAAGTCTGCC
    ACAGAGGCCG AATTAGGGGG TGACGCGTGG CGGCTCTATG AGTACATCAC CAGACACTTC
    ATCGCCACGG TCAGCCATGA CTGCAAGTAC CTGCAGAGCA CCATCTCCTT CAGAATTGGG
    CCCGAGCTCT TCACCTGCTC CGGGAAGACC GTCCTCTCAC CAGGCTTCAC GGAGGTCATG
    CCCTGGCAGA GCGTGCCCCT GGAGGAGAGC CTGCCCACTT GCCAGCGGGG TGATGCCTTC
    CCTGTGGGCG AGGTGAAGAT GCTGGAGAAG CAGACGAACC CACCCGACTA CCTGACGGAG
    GCCGAGCTCA TCACGCTCAT GGAGAAGCAT GGCATCGGCA CGGATGCCAG CATCCCTGTG
    CATATCAACA ACATCTGCCA GCGCAACTAT GTCACGGTGG AGAGCGGGCG CCGGCTCAAG
    CCCACCAACC TCGGCATCGT CCTGGTGCAC GGCTACTATA AGATTGATGC AGAGCTGGTG
    CTCCCCACCA TCCGCAGTGC AGTGGAGAAG CAGCTGAACC TGATCGCCCA GGGCAAGGCC
    GACTACCGCC AGGTCCTGGG CCACACCCTG GACGTGTTCA AGAGGAAGTT CCACTACTTT
    GTCGACTCCA TTGCTGGCAT GGATGAGTTG ATGGAGGTGT CTTTCTCGCC CCTGGCGGCC
    ACAGGCAAGC CCCTCTCACG CTGTGGGAAG TGCCACCGCT TCATGAAGTA CATCCAGGCC
    AAGCCAAGCC GCCTGCACTG CTCCCACTGC GATGAGACCT ACACGCTCCC CCAGAACGGC
    ACCATCAAGC TCTACAAGGA GCTCCGCTGC CCTCTGGATG ACTTCGAGCT GGTCCTGTGG
    TCATCAGGCT CTCGGGGCAA GAGCTACCCG CTGTGCCCCT ACTGCTACAA CCACCCACCC
    TTCCGAGACA TGAAGAAAGG TGAGTGCAGC CACTCCCTCC TGTCCACAGG TAGCTGCAGT
    CTCTTTTCAG TGCCAACCCC TGCCTTGCAC CAGGCTGGAC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102339
    Clone ID Related Accession (Same CDS sequence) NM_001349852.1 , NM_001349851.2 , NM_001349851.1 , NM_001349852.2
    Accession Version NM_001349851.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-10
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-beta-1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA025312.1, DR003213.1, AI361851.1, CN353232.1, CN302239.1, CR980357.1, CF137541.1, CN353231.1, CT002728.1, AK290997.1 and BQ270362.1. On Jun 2, 2019 this sequence version replaced NM_001349851.1. Transcript Variant: This variant (8) differs in its 5' UTR, resulting in the use of a downstream start codon, and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (3) has a shorter N-terminus and a shorter and distinct C-terminus comparted to isoform 1. Variants 8 and 9 encode the same protein (isoform 3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.252110.1, SRR1803612.122711.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158569 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAACAAGG CCCATGGTGG CGAGAAGACC GTGTTCCGGG CCAGGTTTAG CTCCATCACG 
    GACACAGACA TCTGTAATGC CATGGCCTGC CTAGGCGAGC CTGACCACAA CGAGGCGCTC
    TCAGTGGATG CTCGCCAGGA GCTGGACCTG CGAATCGGCT GTGCATTCAC CAGGTTTCAG
    ACTAAATATT TCCAGGGGAA ATACGGTGAT TTAGACAGCT CTCTCATCTC CTTTGGGCCG
    TGTCAGACTC CAACCCTGGG ATTCTGTGTG GAGAGACATG ATAAAATCCA GTCCTTCAAA
    CCAGAGACCT ACTGGGTGCT GCAGGCCAAG GTTAACACTG ACAAAGACAG ATCTCTCCTT
    TTGGACTGGG ACCGAGTAAG AGTGTTTGAC CGGGAGATCG CACAGATGTT TTTAAACATG
    ACAAAGCTGG AGAAGGAAGC CCAGGTGGAG GCCACAAGCA GGAAAGAAAA GGCCAAGCAG
    AGGCCCCTGG CCCTGAACAC TGTGGAGATG CTGCGTGTGG CCAGCTCTTC TCTGGGCATG
    GGGCCGCAGC ACGCCATGCA GACGGCTGAG CGGCTCTACA CGCAAGGCTA CATCAGCTAC
    CCACGGACAG AGACCACCCA CTACCCTGAG AACTTTGACC TGAAGGGCTC TCTGCGGCAG
    CAGGCCAACC ACCCCTACTG GGCCGACACG GTGAAGCGGT TGTTAGCAGA AGGTATCAAC
    CGCCCGCGGA AAGGCCATGA CGCCGGCGAC CATCCCCCCA TCACCCCCAT GAAGTCTGCC
    ACAGAGGCCG AATTAGGGGG TGACGCGTGG CGGCTCTATG AGTACATCAC CAGACACTTC
    ATCGCCACGG TCAGCCATGA CTGCAAGTAC CTGCAGAGCA CCATCTCCTT CAGAATTGGG
    CCCGAGCTCT TCACCTGCTC CGGGAAGACC GTCCTCTCAC CAGGCTTCAC GGAGGTCATG
    CCCTGGCAGA GCGTGCCCCT GGAGGAGAGC CTGCCCACTT GCCAGCGGGG TGATGCCTTC
    CCTGTGGGCG AGGTGAAGAT GCTGGAGAAG CAGACGAACC CACCCGACTA CCTGACGGAG
    GCCGAGCTCA TCACGCTCAT GGAGAAGCAT GGCATCGGCA CGGATGCCAG CATCCCTGTG
    CATATCAACA ACATCTGCCA GCGCAACTAT GTCACGGTGG AGAGCGGGCG CCGGCTCAAG
    CCCACCAACC TCGGCATCGT CCTGGTGCAC GGCTACTATA AGATTGATGC AGAGCTGGTG
    CTCCCCACCA TCCGCAGTGC AGTGGAGAAG CAGCTGAACC TGATCGCCCA GGGCAAGGCC
    GACTACCGCC AGGTCCTGGG CCACACCCTG GACGTGTTCA AGAGGAAGTT CCACTACTTT
    GTCGACTCCA TTGCTGGCAT GGATGAGTTG ATGGAGGTGT CTTTCTCGCC CCTGGCGGCC
    ACAGGCAAGC CCCTCTCACG CTGTGGGAAG TGCCACCGCT TCATGAAGTA CATCCAGGCC
    AAGCCAAGCC GCCTGCACTG CTCCCACTGC GATGAGACCT ACACGCTCCC CCAGAACGGC
    ACCATCAAGC TCTACAAGGA GCTCCGCTGC CCTCTGGATG ACTTCGAGCT GGTCCTGTGG
    TCATCAGGCT CTCGGGGCAA GAGCTACCCG CTGTGCCCCT ACTGCTACAA CCACCCACCC
    TTCCGAGACA TGAAGAAAGG TGAGTGCAGC CACTCCCTCC TGTCCACAGG TAGCTGCAGT
    CTCTTTTCAG TGCCAACCCC TGCCTTGCAC CAGGCTGGAC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336780.1
    CDS647..2431
    Translation

    Target ORF information:

    RefSeq Version NM_001349851.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349851.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAACAAGG CCCATGGTGG CGAGAAGACC GTGTTCCGGG CCAGGTTTAG CTCCATCACG 
    GACACAGACA TCTGTAATGC CATGGCCTGC CTAGGCGAGC CTGACCACAA CGAGGCGCTC
    TCAGTGGATG CTCGCCAGGA GCTGGACCTG CGAATCGGCT GTGCATTCAC CAGGTTTCAG
    ACTAAATATT TCCAGGGGAA ATACGGTGAT TTAGACAGCT CTCTCATCTC CTTTGGGCCG
    TGTCAGACTC CAACCCTGGG ATTCTGTGTG GAGAGACATG ATAAAATCCA GTCCTTCAAA
    CCAGAGACCT ACTGGGTGCT GCAGGCCAAG GTTAACACTG ACAAAGACAG ATCTCTCCTT
    TTGGACTGGG ACCGAGTAAG AGTGTTTGAC CGGGAGATCG CACAGATGTT TTTAAACATG
    ACAAAGCTGG AGAAGGAAGC CCAGGTGGAG GCCACAAGCA GGAAAGAAAA GGCCAAGCAG
    AGGCCCCTGG CCCTGAACAC TGTGGAGATG CTGCGTGTGG CCAGCTCTTC TCTGGGCATG
    GGGCCGCAGC ACGCCATGCA GACGGCTGAG CGGCTCTACA CGCAAGGCTA CATCAGCTAC
    CCACGGACAG AGACCACCCA CTACCCTGAG AACTTTGACC TGAAGGGCTC TCTGCGGCAG
    CAGGCCAACC ACCCCTACTG GGCCGACACG GTGAAGCGGT TGTTAGCAGA AGGTATCAAC
    CGCCCGCGGA AAGGCCATGA CGCCGGCGAC CATCCCCCCA TCACCCCCAT GAAGTCTGCC
    ACAGAGGCCG AATTAGGGGG TGACGCGTGG CGGCTCTATG AGTACATCAC CAGACACTTC
    ATCGCCACGG TCAGCCATGA CTGCAAGTAC CTGCAGAGCA CCATCTCCTT CAGAATTGGG
    CCCGAGCTCT TCACCTGCTC CGGGAAGACC GTCCTCTCAC CAGGCTTCAC GGAGGTCATG
    CCCTGGCAGA GCGTGCCCCT GGAGGAGAGC CTGCCCACTT GCCAGCGGGG TGATGCCTTC
    CCTGTGGGCG AGGTGAAGAT GCTGGAGAAG CAGACGAACC CACCCGACTA CCTGACGGAG
    GCCGAGCTCA TCACGCTCAT GGAGAAGCAT GGCATCGGCA CGGATGCCAG CATCCCTGTG
    CATATCAACA ACATCTGCCA GCGCAACTAT GTCACGGTGG AGAGCGGGCG CCGGCTCAAG
    CCCACCAACC TCGGCATCGT CCTGGTGCAC GGCTACTATA AGATTGATGC AGAGCTGGTG
    CTCCCCACCA TCCGCAGTGC AGTGGAGAAG CAGCTGAACC TGATCGCCCA GGGCAAGGCC
    GACTACCGCC AGGTCCTGGG CCACACCCTG GACGTGTTCA AGAGGAAGTT CCACTACTTT
    GTCGACTCCA TTGCTGGCAT GGATGAGTTG ATGGAGGTGT CTTTCTCGCC CCTGGCGGCC
    ACAGGCAAGC CCCTCTCACG CTGTGGGAAG TGCCACCGCT TCATGAAGTA CATCCAGGCC
    AAGCCAAGCC GCCTGCACTG CTCCCACTGC GATGAGACCT ACACGCTCCC CCAGAACGGC
    ACCATCAAGC TCTACAAGGA GCTCCGCTGC CCTCTGGATG ACTTCGAGCT GGTCCTGTGG
    TCATCAGGCT CTCGGGGCAA GAGCTACCCG CTGTGCCCCT ACTGCTACAA CCACCCACCC
    TTCCGAGACA TGAAGAAAGG TGAGTGCAGC CACTCCCTCC TGTCCACAGG TAGCTGCAGT
    CTCTTTTCAG TGCCAACCCC TGCCTTGCAC CAGGCTGGAC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102339
    Clone ID Related Accession (Same CDS sequence) NM_001349852.1 , NM_001349851.2 , NM_001349851.1 , NM_001349852.2
    Accession Version NM_001349851.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-03
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-beta-1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA025312.1, DR003213.1, AI361851.1, CN353232.1, CN302239.1, CR980357.1, CF137541.1, CN353231.1, CT002728.1, AK290997.1 and BQ270362.1. On Mar 28, 2017 this sequence version replaced XM_017029040.1. Transcript Variant: This variant (8) differs in its 5' UTR, resulting in the use of a downstream start codon, and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (3) has a shorter N-terminus and a shorter and distinct C-terminus comparted to isoform 1. Variants 8 and 9 encode the same protein (isoform 3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.252110.1, SRR1803612.122711.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158569 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAACAAGG CCCATGGTGG CGAGAAGACC GTGTTCCGGG CCAGGTTTAG CTCCATCACG 
    GACACAGACA TCTGTAATGC CATGGCCTGC CTAGGCGAGC CTGACCACAA CGAGGCGCTC
    TCAGTGGATG CTCGCCAGGA GCTGGACCTG CGAATCGGCT GTGCATTCAC CAGGTTTCAG
    ACTAAATATT TCCAGGGGAA ATACGGTGAT TTAGACAGCT CTCTCATCTC CTTTGGGCCG
    TGTCAGACTC CAACCCTGGG ATTCTGTGTG GAGAGACATG ATAAAATCCA GTCCTTCAAA
    CCAGAGACCT ACTGGGTGCT GCAGGCCAAG GTTAACACTG ACAAAGACAG ATCTCTCCTT
    TTGGACTGGG ACCGAGTAAG AGTGTTTGAC CGGGAGATCG CACAGATGTT TTTAAACATG
    ACAAAGCTGG AGAAGGAAGC CCAGGTGGAG GCCACAAGCA GGAAAGAAAA GGCCAAGCAG
    AGGCCCCTGG CCCTGAACAC TGTGGAGATG CTGCGTGTGG CCAGCTCTTC TCTGGGCATG
    GGGCCGCAGC ACGCCATGCA GACGGCTGAG CGGCTCTACA CGCAAGGCTA CATCAGCTAC
    CCACGGACAG AGACCACCCA CTACCCTGAG AACTTTGACC TGAAGGGCTC TCTGCGGCAG
    CAGGCCAACC ACCCCTACTG GGCCGACACG GTGAAGCGGT TGTTAGCAGA AGGTATCAAC
    CGCCCGCGGA AAGGCCATGA CGCCGGCGAC CATCCCCCCA TCACCCCCAT GAAGTCTGCC
    ACAGAGGCCG AATTAGGGGG TGACGCGTGG CGGCTCTATG AGTACATCAC CAGACACTTC
    ATCGCCACGG TCAGCCATGA CTGCAAGTAC CTGCAGAGCA CCATCTCCTT CAGAATTGGG
    CCCGAGCTCT TCACCTGCTC CGGGAAGACC GTCCTCTCAC CAGGCTTCAC GGAGGTCATG
    CCCTGGCAGA GCGTGCCCCT GGAGGAGAGC CTGCCCACTT GCCAGCGGGG TGATGCCTTC
    CCTGTGGGCG AGGTGAAGAT GCTGGAGAAG CAGACGAACC CACCCGACTA CCTGACGGAG
    GCCGAGCTCA TCACGCTCAT GGAGAAGCAT GGCATCGGCA CGGATGCCAG CATCCCTGTG
    CATATCAACA ACATCTGCCA GCGCAACTAT GTCACGGTGG AGAGCGGGCG CCGGCTCAAG
    CCCACCAACC TCGGCATCGT CCTGGTGCAC GGCTACTATA AGATTGATGC AGAGCTGGTG
    CTCCCCACCA TCCGCAGTGC AGTGGAGAAG CAGCTGAACC TGATCGCCCA GGGCAAGGCC
    GACTACCGCC AGGTCCTGGG CCACACCCTG GACGTGTTCA AGAGGAAGTT CCACTACTTT
    GTCGACTCCA TTGCTGGCAT GGATGAGTTG ATGGAGGTGT CTTTCTCGCC CCTGGCGGCC
    ACAGGCAAGC CCCTCTCACG CTGTGGGAAG TGCCACCGCT TCATGAAGTA CATCCAGGCC
    AAGCCAAGCC GCCTGCACTG CTCCCACTGC GATGAGACCT ACACGCTCCC CCAGAACGGC
    ACCATCAAGC TCTACAAGGA GCTCCGCTGC CCTCTGGATG ACTTCGAGCT GGTCCTGTGG
    TCATCAGGCT CTCGGGGCAA GAGCTACCCG CTGTGCCCCT ACTGCTACAA CCACCCACCC
    TTCCGAGACA TGAAGAAAGG TGAGTGCAGC CACTCCCTCC TGTCCACAGG TAGCTGCAGT
    CTCTTTTCAG TGCCAACCCC TGCCTTGCAC CAGGCTGGAC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336780.1
    CDS703..2487
    Translation

    Target ORF information:

    RefSeq Version NM_001349851.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349851.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAACAAGG CCCATGGTGG CGAGAAGACC GTGTTCCGGG CCAGGTTTAG CTCCATCACG 
    GACACAGACA TCTGTAATGC CATGGCCTGC CTAGGCGAGC CTGACCACAA CGAGGCGCTC
    TCAGTGGATG CTCGCCAGGA GCTGGACCTG CGAATCGGCT GTGCATTCAC CAGGTTTCAG
    ACTAAATATT TCCAGGGGAA ATACGGTGAT TTAGACAGCT CTCTCATCTC CTTTGGGCCG
    TGTCAGACTC CAACCCTGGG ATTCTGTGTG GAGAGACATG ATAAAATCCA GTCCTTCAAA
    CCAGAGACCT ACTGGGTGCT GCAGGCCAAG GTTAACACTG ACAAAGACAG ATCTCTCCTT
    TTGGACTGGG ACCGAGTAAG AGTGTTTGAC CGGGAGATCG CACAGATGTT TTTAAACATG
    ACAAAGCTGG AGAAGGAAGC CCAGGTGGAG GCCACAAGCA GGAAAGAAAA GGCCAAGCAG
    AGGCCCCTGG CCCTGAACAC TGTGGAGATG CTGCGTGTGG CCAGCTCTTC TCTGGGCATG
    GGGCCGCAGC ACGCCATGCA GACGGCTGAG CGGCTCTACA CGCAAGGCTA CATCAGCTAC
    CCACGGACAG AGACCACCCA CTACCCTGAG AACTTTGACC TGAAGGGCTC TCTGCGGCAG
    CAGGCCAACC ACCCCTACTG GGCCGACACG GTGAAGCGGT TGTTAGCAGA AGGTATCAAC
    CGCCCGCGGA AAGGCCATGA CGCCGGCGAC CATCCCCCCA TCACCCCCAT GAAGTCTGCC
    ACAGAGGCCG AATTAGGGGG TGACGCGTGG CGGCTCTATG AGTACATCAC CAGACACTTC
    ATCGCCACGG TCAGCCATGA CTGCAAGTAC CTGCAGAGCA CCATCTCCTT CAGAATTGGG
    CCCGAGCTCT TCACCTGCTC CGGGAAGACC GTCCTCTCAC CAGGCTTCAC GGAGGTCATG
    CCCTGGCAGA GCGTGCCCCT GGAGGAGAGC CTGCCCACTT GCCAGCGGGG TGATGCCTTC
    CCTGTGGGCG AGGTGAAGAT GCTGGAGAAG CAGACGAACC CACCCGACTA CCTGACGGAG
    GCCGAGCTCA TCACGCTCAT GGAGAAGCAT GGCATCGGCA CGGATGCCAG CATCCCTGTG
    CATATCAACA ACATCTGCCA GCGCAACTAT GTCACGGTGG AGAGCGGGCG CCGGCTCAAG
    CCCACCAACC TCGGCATCGT CCTGGTGCAC GGCTACTATA AGATTGATGC AGAGCTGGTG
    CTCCCCACCA TCCGCAGTGC AGTGGAGAAG CAGCTGAACC TGATCGCCCA GGGCAAGGCC
    GACTACCGCC AGGTCCTGGG CCACACCCTG GACGTGTTCA AGAGGAAGTT CCACTACTTT
    GTCGACTCCA TTGCTGGCAT GGATGAGTTG ATGGAGGTGT CTTTCTCGCC CCTGGCGGCC
    ACAGGCAAGC CCCTCTCACG CTGTGGGAAG TGCCACCGCT TCATGAAGTA CATCCAGGCC
    AAGCCAAGCC GCCTGCACTG CTCCCACTGC GATGAGACCT ACACGCTCCC CCAGAACGGC
    ACCATCAAGC TCTACAAGGA GCTCCGCTGC CCTCTGGATG ACTTCGAGCT GGTCCTGTGG
    TCATCAGGCT CTCGGGGCAA GAGCTACCCG CTGTGCCCCT ACTGCTACAA CCACCCACCC
    TTCCGAGACA TGAAGAAAGG TGAGTGCAGC CACTCCCTCC TGTCCACAGG TAGCTGCAGT
    CTCTTTTCAG TGCCAACCCC TGCCTTGCAC CAGGCTGGAC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102339
    Clone ID Related Accession (Same CDS sequence) NM_001349852.1 , NM_001349851.2 , NM_001349851.1 , NM_001349852.2
    Accession Version NM_001349852.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-11
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-beta-1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA025312.1, DA124773.1, DB146985.1, DB150453.1, DR003213.1, AI361851.1, CN353232.1, CN302239.1, CR980357.1, CF137541.1, CN353231.1, CT002728.1, AK290997.1 and BQ270362.1. On Jun 2, 2019 this sequence version replaced NM_001349852.1. Transcript Variant: This variant (9) differs in its 5' UTR, resulting in the use of a downstream start codon, and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (3) has a shorter N-terminus and a shorter and distinct C-terminus comparted to isoform 1. Variants 8 and 9 encode the same protein (isoform 3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.20507.1, SRR1803614.180278.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAACAAGG CCCATGGTGG CGAGAAGACC GTGTTCCGGG CCAGGTTTAG CTCCATCACG 
    GACACAGACA TCTGTAATGC CATGGCCTGC CTAGGCGAGC CTGACCACAA CGAGGCGCTC
    TCAGTGGATG CTCGCCAGGA GCTGGACCTG CGAATCGGCT GTGCATTCAC CAGGTTTCAG
    ACTAAATATT TCCAGGGGAA ATACGGTGAT TTAGACAGCT CTCTCATCTC CTTTGGGCCG
    TGTCAGACTC CAACCCTGGG ATTCTGTGTG GAGAGACATG ATAAAATCCA GTCCTTCAAA
    CCAGAGACCT ACTGGGTGCT GCAGGCCAAG GTTAACACTG ACAAAGACAG ATCTCTCCTT
    TTGGACTGGG ACCGAGTAAG AGTGTTTGAC CGGGAGATCG CACAGATGTT TTTAAACATG
    ACAAAGCTGG AGAAGGAAGC CCAGGTGGAG GCCACAAGCA GGAAAGAAAA GGCCAAGCAG
    AGGCCCCTGG CCCTGAACAC TGTGGAGATG CTGCGTGTGG CCAGCTCTTC TCTGGGCATG
    GGGCCGCAGC ACGCCATGCA GACGGCTGAG CGGCTCTACA CGCAAGGCTA CATCAGCTAC
    CCACGGACAG AGACCACCCA CTACCCTGAG AACTTTGACC TGAAGGGCTC TCTGCGGCAG
    CAGGCCAACC ACCCCTACTG GGCCGACACG GTGAAGCGGT TGTTAGCAGA AGGTATCAAC
    CGCCCGCGGA AAGGCCATGA CGCCGGCGAC CATCCCCCCA TCACCCCCAT GAAGTCTGCC
    ACAGAGGCCG AATTAGGGGG TGACGCGTGG CGGCTCTATG AGTACATCAC CAGACACTTC
    ATCGCCACGG TCAGCCATGA CTGCAAGTAC CTGCAGAGCA CCATCTCCTT CAGAATTGGG
    CCCGAGCTCT TCACCTGCTC CGGGAAGACC GTCCTCTCAC CAGGCTTCAC GGAGGTCATG
    CCCTGGCAGA GCGTGCCCCT GGAGGAGAGC CTGCCCACTT GCCAGCGGGG TGATGCCTTC
    CCTGTGGGCG AGGTGAAGAT GCTGGAGAAG CAGACGAACC CACCCGACTA CCTGACGGAG
    GCCGAGCTCA TCACGCTCAT GGAGAAGCAT GGCATCGGCA CGGATGCCAG CATCCCTGTG
    CATATCAACA ACATCTGCCA GCGCAACTAT GTCACGGTGG AGAGCGGGCG CCGGCTCAAG
    CCCACCAACC TCGGCATCGT CCTGGTGCAC GGCTACTATA AGATTGATGC AGAGCTGGTG
    CTCCCCACCA TCCGCAGTGC AGTGGAGAAG CAGCTGAACC TGATCGCCCA GGGCAAGGCC
    GACTACCGCC AGGTCCTGGG CCACACCCTG GACGTGTTCA AGAGGAAGTT CCACTACTTT
    GTCGACTCCA TTGCTGGCAT GGATGAGTTG ATGGAGGTGT CTTTCTCGCC CCTGGCGGCC
    ACAGGCAAGC CCCTCTCACG CTGTGGGAAG TGCCACCGCT TCATGAAGTA CATCCAGGCC
    AAGCCAAGCC GCCTGCACTG CTCCCACTGC GATGAGACCT ACACGCTCCC CCAGAACGGC
    ACCATCAAGC TCTACAAGGA GCTCCGCTGC CCTCTGGATG ACTTCGAGCT GGTCCTGTGG
    TCATCAGGCT CTCGGGGCAA GAGCTACCCG CTGTGCCCCT ACTGCTACAA CCACCCACCC
    TTCCGAGACA TGAAGAAAGG TGAGTGCAGC CACTCCCTCC TGTCCACAGG TAGCTGCAGT
    CTCTTTTCAG TGCCAACCCC TGCCTTGCAC CAGGCTGGAC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336781.1
    CDS683..2467
    Translation

    Target ORF information:

    RefSeq Version NM_001349852.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349852.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAACAAGG CCCATGGTGG CGAGAAGACC GTGTTCCGGG CCAGGTTTAG CTCCATCACG 
    GACACAGACA TCTGTAATGC CATGGCCTGC CTAGGCGAGC CTGACCACAA CGAGGCGCTC
    TCAGTGGATG CTCGCCAGGA GCTGGACCTG CGAATCGGCT GTGCATTCAC CAGGTTTCAG
    ACTAAATATT TCCAGGGGAA ATACGGTGAT TTAGACAGCT CTCTCATCTC CTTTGGGCCG
    TGTCAGACTC CAACCCTGGG ATTCTGTGTG GAGAGACATG ATAAAATCCA GTCCTTCAAA
    CCAGAGACCT ACTGGGTGCT GCAGGCCAAG GTTAACACTG ACAAAGACAG ATCTCTCCTT
    TTGGACTGGG ACCGAGTAAG AGTGTTTGAC CGGGAGATCG CACAGATGTT TTTAAACATG
    ACAAAGCTGG AGAAGGAAGC CCAGGTGGAG GCCACAAGCA GGAAAGAAAA GGCCAAGCAG
    AGGCCCCTGG CCCTGAACAC TGTGGAGATG CTGCGTGTGG CCAGCTCTTC TCTGGGCATG
    GGGCCGCAGC ACGCCATGCA GACGGCTGAG CGGCTCTACA CGCAAGGCTA CATCAGCTAC
    CCACGGACAG AGACCACCCA CTACCCTGAG AACTTTGACC TGAAGGGCTC TCTGCGGCAG
    CAGGCCAACC ACCCCTACTG GGCCGACACG GTGAAGCGGT TGTTAGCAGA AGGTATCAAC
    CGCCCGCGGA AAGGCCATGA CGCCGGCGAC CATCCCCCCA TCACCCCCAT GAAGTCTGCC
    ACAGAGGCCG AATTAGGGGG TGACGCGTGG CGGCTCTATG AGTACATCAC CAGACACTTC
    ATCGCCACGG TCAGCCATGA CTGCAAGTAC CTGCAGAGCA CCATCTCCTT CAGAATTGGG
    CCCGAGCTCT TCACCTGCTC CGGGAAGACC GTCCTCTCAC CAGGCTTCAC GGAGGTCATG
    CCCTGGCAGA GCGTGCCCCT GGAGGAGAGC CTGCCCACTT GCCAGCGGGG TGATGCCTTC
    CCTGTGGGCG AGGTGAAGAT GCTGGAGAAG CAGACGAACC CACCCGACTA CCTGACGGAG
    GCCGAGCTCA TCACGCTCAT GGAGAAGCAT GGCATCGGCA CGGATGCCAG CATCCCTGTG
    CATATCAACA ACATCTGCCA GCGCAACTAT GTCACGGTGG AGAGCGGGCG CCGGCTCAAG
    CCCACCAACC TCGGCATCGT CCTGGTGCAC GGCTACTATA AGATTGATGC AGAGCTGGTG
    CTCCCCACCA TCCGCAGTGC AGTGGAGAAG CAGCTGAACC TGATCGCCCA GGGCAAGGCC
    GACTACCGCC AGGTCCTGGG CCACACCCTG GACGTGTTCA AGAGGAAGTT CCACTACTTT
    GTCGACTCCA TTGCTGGCAT GGATGAGTTG ATGGAGGTGT CTTTCTCGCC CCTGGCGGCC
    ACAGGCAAGC CCCTCTCACG CTGTGGGAAG TGCCACCGCT TCATGAAGTA CATCCAGGCC
    AAGCCAAGCC GCCTGCACTG CTCCCACTGC GATGAGACCT ACACGCTCCC CCAGAACGGC
    ACCATCAAGC TCTACAAGGA GCTCCGCTGC CCTCTGGATG ACTTCGAGCT GGTCCTGTGG
    TCATCAGGCT CTCGGGGCAA GAGCTACCCG CTGTGCCCCT ACTGCTACAA CCACCCACCC
    TTCCGAGACA TGAAGAAAGG TGAGTGCAGC CACTCCCTCC TGTCCACAGG TAGCTGCAGT
    CTCTTTTCAG TGCCAACCCC TGCCTTGCAC CAGGCTGGAC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93632
    Clone ID Related Accession (Same CDS sequence) XM_017029038.2 , XM_017029038.1
    Accession Version XM_017029038.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2184bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-beta-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017029038.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGCCA CCCGAGGAGA GACACCTGTG CGGGATCCAC TGTGTGTTGG AAACTCCCTC
    CACACTCCGC CCGTGCCTCA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884527.1
    CDS183..2366
    Translation

    Target ORF information:

    RefSeq Version XM_017029038.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029038.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGCCA CCCGAGGAGA GACACCTGTG CGGGATCCAC TGTGTGTTGG AAACTCCCTC
    CACACTCCGC CCGTGCCTCA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93632
    Clone ID Related Accession (Same CDS sequence) XM_017029038.2 , XM_017029038.1
    Accession Version XM_017029038.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2184bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-beta-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGCCA CCCGAGGAGA GACACCTGTG CGGGATCCAC TGTGTGTTGG AAACTCCCTC
    CACACTCCGC CCGTGCCTCA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884527.1
    CDS192..2375
    Translation

    Target ORF information:

    RefSeq Version XM_017029038.1
    Organism Homo sapiens(human)
    Definition Homo sapiens topoisomerase (DNA) III beta (TOP3B), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029038.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAAGACTG TGCTCATGGT TGCTGAAAAG CCGTCCTTGG CACAGTCAAT TGCCAAAATC 
    CTCTCTAGAG GGAGCCTGTC CTCACACAAA GGGCTGAACG GGGCCTGCTC AGTCCACGAG
    TACACTGGGA CCTTTGCTGG CCAGCCAGTG CGCTTCAAGA TGACGTCTGT CTGTGGTCAC
    GTGATGACCC TGGATTTCCT GGGAAAATAC AACAAATGGG ACAAAGTGGA CCCCGCAGAA
    CTGTTCAGCC AAGCTCCCAC GGAGAAGAAA GAAGCTAACC CCAAGCTGAA CATGGTGAAG
    TTCCTGCAGG TGGAGGGCAG AGGCTGCGAC TACATCGTGC TGTGGCTGGA CTGCGACAAG
    GAGGGGGAGA ACATCTGCTT TGAGGTTCTT GATGCTGTTC TGCCCGTCAT GAACAAGGCC
    CATGGTGGCG AGAAGACCGT GTTCCGGGCC AGGTTTAGCT CCATCACGGA CACAGACATC
    TGTAATGCCA TGGCCTGCCT AGGCGAGCCT GACCACAACG AGGCGCTCTC AGTGGATGCT
    CGCCAGGAGC TGGACCTGCG AATCGGCTGT GCATTCACCA GGTTTCAGAC TAAATATTTC
    CAGGGGAAAT ACGGTGATTT AGACAGCTCT CTCATCTCCT TTGGGCCGTG TCAGACTCCA
    ACCCTGGGAT TCTGTGTGGA GAGACATGAT AAAATCCAGT CCTTCAAACC AGAGACCTAC
    TGGGTGCTGC AGGCCAAGGT TAACACTGAC AAAGACAGAT CTCTCCTTTT GGACTGGGAC
    CGAGTAAGAG TGTTTGACCG GGAGATCGCA CAGATGTTTT TAAACATGAC AAAGCTGGAG
    AAGGAAGCCC AGGTGGAGGC CACAAGCAGG AAAGAAAAGG CCAAGCAGAG GCCCCTGGCC
    CTGAACACTG TGGAGATGCT GCGTGTGGCC AGCTCTTCTC TGGGCATGGG GCCGCAGCAC
    GCCATGCAGA CGGCTGAGCG GCTCTACACG CAAGGCTACA TCAGCTACCC ACGGACAGAG
    ACCACCCACT ACCCTGAGAA CTTTGACCTG AAGGGCTCTC TGCGGCAGCA GGCCAACCAC
    CCCTACTGGG CCGACACGGT GAAGCGGTTG TTAGCAGAAG GTATCAACCG CCCGCGGAAA
    GGCCATGACG CCGGCGACCA TCCCCCCATC ACCCCCATGA AGTCTGCCAC AGAGGCCGAA
    TTAGGGGGTG ACGCGTGGCG GCTCTATGAG TACATCACCA GACACTTCAT CGCCACGGTC
    AGCCATGACT GCAAGTACCT GCAGAGCACC ATCTCCTTCA GAATTGGGCC CGAGCTCTTC
    ACCTGCTCCG GGAAGACCGT CCTCTCACCA GGCTTCACGG AGGTCATGCC CTGGCAGAGC
    GTGCCCCTGG AGGAGAGCCT GCCCACTTGC CAGCGGGGTG ATGCCTTCCC TGTGGGCGAG
    GTGAAGATGC TGGAGAAGCA GACGAACCCA CCCGACTACC TGACGGAGGC CGAGCTCATC
    ACGCTCATGG AGAAGCATGG CATCGGCACG GATGCCAGCA TCCCTGTGCA TATCAACAAC
    ATCTGCCAGC GCAACTATGT CACGGTGGAG AGCGGGCGCC GGCTCAAGCC CACCAACCTC
    GGCATCGTCC TGGTGCACGG CTACTATAAG ATTGATGCAG AGCTGGTGCT CCCCACCATC
    CGCAGTGCAG TGGAGAAGCA GCTGAACCTG ATCGCCCAGG GCAAGGCCGA CTACCGCCAG
    GTCCTGGGCC ACACCCTGGA CGTGTTCAAG AGGAAGTTCC ACTACTTTGT CGACTCCATT
    GCTGGCATGG ATGAGTTGAT GGAGGTGTCT TTCTCGCCCC TGGCGGCCAC AGGCAAGCCC
    CTCTCACGCT GTGGGAAGTG CCACCGCTTC ATGAAGTACA TCCAGGCCAA GCCAAGCCGC
    CTGCACTGCT CCCACTGCGA TGAGACCTAC ACGCTCCCCC AGAACGGCAC CATCAAGCTC
    TACAAGGAGC TCCGCTGCCC TCTGGATGAC TTCGAGCTGG TCCTGTGGTC ATCAGGCTCT
    CGGGGCAAGA GCTACCCGCT GTGCCCCTAC TGCTACAACC ACCCACCCTT CCGAGACATG
    AAGAAAGCCA CCCGAGGAGA GACACCTGTG CGGGATCCAC TGTGTGTTGG AAACTCCCTC
    CACACTCCGC CCGTGCCTCA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.